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Sample records for progeny recurrent selection

  1. Prediction of genetic gains from selection in Arabica coffee progenies.

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    Antonio Carlos Baião de Oliveira

    2011-01-01

    Full Text Available Gains from selection for yield were estimated in Arabica coffee progenies carrying rust-resistance genes. Theexperiment in augmented block design was installed in Três Pontas, state of Minas Gerais. Three blocks were established with sixplants per plot, spaced 3.50 x 0.90 m, in 96 regular (F2 progenies and two control treatments. The plant response to rust wasevaluated on a grade scale in 2008. Yield (bags per hectare was estimated in the growing seasons 2005 to 2008. Significantdifferences between treatments for yield were observed in all harvests, except 2005. The presence of genetic variability amongprogenies allowed significant gain from selection for yield. Under the experimental conditions of this study, selection for yield can beperformed in the first high-yield year, without major losses compared to genetic gain from selection for yield when based on the meanof four harvests.

  2. Selection of carioca common bean progenies resistant to white mold

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    Renato Sérgio Batista Carvalho

    2013-01-01

    Full Text Available A backcross breeding program between commercial common bean cultivars (VC3 and M20 and sources of resistance(Ex-Rico 23 and G122 was conducted with a view toward selecting carioca (beige with brown stripes progenies resistant to whitemold. Forty-eight progenies (27 F2:6 BC1 and 21 F1:5 BC2 were evaluated for yield, growth habit, grain type and pathogen responseby two methodologies for assessment (“straw test” and “oxalic acid”. The methods were effective in discriminating the progenies,showing differing results, for they may assess different resistance mechanisms. Thus, they should be used together. Simultaneousselection for yield, growth habit, grain type and white mold resistance proved to be viable. The most appropriate strategy was to uselower intensities of selection and prioritize traits such as resistance and grain type, which are essential for commercial acceptance.Two progenies proved their superiority for the breeding program for they combine the traits of resistance and favorable grain type.

  3. Phytochemical profile of morphologically selected yerba-mate progenies

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    Alice Teresa Valduga

    2016-02-01

    Full Text Available ABSTRACT Yerba-mate (Ilex paraguariensis St. Hil is a native South American species. Plant progenies are populations that differ in terms of their productivity, morphology and phytochemical profile. This study aimed to determine the concentration of primary and secondary metabolites, such as antioxidants, in leaves, of yerba-mate progenies selected based on morphological characteristics. We evaluated the centesimal composition of secondary metabolites in the leaves of five yerba-mate plants. Methylxanthines and phenolic compounds were determined by UPLC-PDA, and antioxidant activity by measuring DPPH scavenging. Significant differences were found in centesimal composition and the contents of caffeine, theobromine, rutin and chlorogenic acid, as well as antioxidant activities, in selected progenies. The IC50 values were correlated with the chlorogenic acid levels (r2 = 0.5242 and soluble content (r2 = 0.7686. The morphological characteristics observed in yerba-mate leaves can be used as a tool for plant selection, to obtain matrices with different phytochemical profiles as a genetic material source.

  4. Selection of arabica coffee progenies tolerant to heat stress

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    Alexsandro Lara Teixeira

    2015-07-01

    Full Text Available Due to high temperatures, practically all coffee farms in the state of Rondonia are of the C. canephora species. Thus, importing arabica coffee from other states becomes necessary for composition of blends, as well as for the specialty or gourmet coffee market. The purpose of this study was to select arabica coffee genotypes that exhibit satisfactory agronomic performance under high temperature conditions. The experiment was conducted in OuroPreto do Oeste, RO, Brazil, with mean annual temperature of 25.8°C and mean annual rainfall of 2300mm year-1. The experiment was composed of 114 arabica coffee genotypes, with 103 progenies and eleven control cultivars, provided by EPAMIG. A randomized block experimental design was used with three replications, spacing of 3.0x1.0 meters and five plants per plot. All the crop seasons showed significant difference for the green coffee yield trait. In joint analysis, significant differences were detected among progenies and control cultivars. In the average of the four harvests, green coffee yield was 32.38 bags ha-1. The cultivars 'CatuaíVermelho IAC 15', 'Obatã IAC 1669-20' and 'Catucaí Amarelo 2SLCAK' stood out, achieving yields greater than 40 bags ha-1. The gain obtained from selection was 14.33 bags ha-1, which is equivalent to an increase of 44.04% in production of green coffee. The progeny H514-7-10-6-2-3-9 stood out with an average yield of 51.20 bags ha-1. In regard to maturation cycle, 56% of the progenies were classified as early maturity and 44% as medium maturity. Late maturity genotypes were not observed

  5. Proposal of a super trait for the optimum selection of popcorn progenies based on path analysis.

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    do Amaral Júnior, A T; Dos Santos, A; Gerhardt, I F S; Kurosawa, R N F; Moreira, N F; Pereira, M G; de A Gravina, G; de L Silva, F H

    2016-12-19

    A challenge faced by popcorn breeding programs is the existence of a negative correlation between the two main traits, popping expansion and yield, which hinders simultaneous gains. The objective of this study was to investigate the use of a new variable or super trait, which favors the reliable selection of superior progenies. The super trait 'expanded popcorn volume per hectare' was introduced in the evaluation of 200 full-sib families of the eighth recurrent intrapopulation selection cycle, which were arranged in randomized blocks with three replicates in two environments. Although the inability to obtain simultaneous gains through selection via popping expansion or yield was confirmed, the super trait was positively associated with both yield and popping expansion, allowing simultaneous gains via indirect selection using 'expanded popcorn volume per hectare' as the main trait. This approach is recommended because this super trait can be used in breeding programs to optimize selective gains for the crop.

  6. Mixed models for selection of Jatropha progenies with high adaptability and yield stability in Brazilian regions.

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    Teodoro, P E; Bhering, L L; Costa, R D; Rocha, R B; Laviola, B G

    2016-08-19

    The aim of this study was to estimate genetic parameters via mixed models and simultaneously to select Jatropha progenies grown in three regions of Brazil that meet high adaptability and stability. From a previous phenotypic selection, three progeny tests were installed in 2008 in the municipalities of Planaltina-DF (Midwest), Nova Porteirinha-MG (Southeast), and Pelotas-RS (South). We evaluated 18 families of half-sib in a randomized block design with three replications. Genetic parameters were estimated using restricted maximum likelihood/best linear unbiased prediction. Selection was based on the harmonic mean of the relative performance of genetic values method in three strategies considering: 1) performance in each environment (with interaction effect); 2) performance in each environment (with interaction effect); and 3) simultaneous selection for grain yield, stability and adaptability. Accuracy obtained (91%) reveals excellent experimental quality and consequently safety and credibility in the selection of superior progenies for grain yield. The gain with the selection of the best five progenies was more than 20%, regardless of the selection strategy. Thus, based on the three selection strategies used in this study, the progenies 4, 11, and 3 (selected in all environments and the mean environment and by adaptability and phenotypic stability methods) are the most suitable for growing in the three regions evaluated.

  7. Improvement in genetic characteristics and oil yield of selected soybean progenies from octuple crosses

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    Hamawaki Osvaldo Toshiyuki

    2000-01-01

    Full Text Available The objective of the present study was to evaluate 44 soybean octuple crosses in the F4:3[8] and F5:3[8] generations in order to select progenies superior for seed oil yield (OY and other important agronomic characteristics. Octuple crosses were hybridized in a chain mating system. In one group, crosses were carried out for three generations with the adapted x exotic parents until octuple crosses with 75% adapted genes and 25% exotic genes were obtained. In a second group, hybridization of adapted x adapted parents originated crosses with 100% adapted genes. During the growing season 1994/95, the progenies F4:3[8] were evaluated by using the augmented block design. The progenies F5:3[8] were evaluated during the growing season 1995/96 in three experiments using augmented block design without repetition. The octuple crosses gave origin to superior progenies for all the characters studied. In the C22 cross, OY values were 707 kg/ha. The estimates of heritability in relation to the crosses average resulted in the following mean, minimum and maximum values, respectively: number of days to maturity (52.35%, 3.71%, 84.23%; agronomic value (26.69%, 1.62%, 61.28% and grain yield (29.28%, 1.52%, 61.06%. The observed genetic gains for grain yield in the early, intermediate and late F5:3[8] progenies were superior to the expected genetic gains and the observed genetic gains for OY were more expressive in the early and late F5:[8] progenies. The genetic variability remaining in the selected progenies of some crosses suggests that further genetic gains for grain yield and OY might be possible with advanced selection cycles.

  8. Genetic potential of common bean progenies selected for crude fiber content obtained through different breeding methods.

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    Júnior, V A P; Melo, P G S; Pereira, H S; Bassinello, P Z; Melo, L C

    2015-05-29

    Gastrointestinal health is of great importance due to the increasing consumption of functional foods, especially those concern-ing diets rich in fiber content. The common bean has been valorized as a nutritious food due to its appreciable fiber content and the fact that it is consumed in many countries. The current study aimed to evaluate and compare the genetic potential of common bean progenies of the carioca group, developed through different breeding methods, for crude fiber content. The progenies originated through hybridization of two advanced strains, CNFC 7812 and CNFC 7829, up to the F7 generation using three breeding methods: bulk-population, bulk within F2 families, and single seed descent. Fifteen F8 progenies were evaluated in each method, as well as two check cultivars and both parents, us-ing a 7 x 7 simple lattice design, with experimental plots comprised of two 4-m long rows. Field trials were conducted in eleven environments encompassing four Brazilian states and three different sowing times during 2009 and 2010. Estimates of genetic parameters indicate differences among the breeding methods, which seem to be related to the different processes for sampling the advanced progenies inherent to each method, given that the trait in question is not subject to natural selection. Variability amongst progenies occurred within the three breeding methods and there was also a significant effect of environment on the progeny for all methods. Progenies developed by bulk-population attained the highest estimates of genetic parameters, had less interaction with the environment, and greater variability.

  9. Indices estimated using REML/BLUP and introduction of a super-trait for the selection of progenies in popcorn.

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    Vittorazzi, C; Amaral Junior, A T; Guimarães, A G; Viana, A P; Silva, F H L; Pena, G F; Daher, R F; Gerhardt, I F S; Oliveira, G H F; Pereira, M G

    2017-09-27

    Selection indices commonly utilize economic weights, which become arbitrary genetic gains. In popcorn, this is even more evident due to the negative correlation between the main characteristics of economic importance - grain yield and popping expansion. As an option in the use of classical biometrics as a selection index, the optimal procedure restricted maximum likelihood/best linear unbiased predictor (REML/BLUP) allows the simultaneous estimation of genetic parameters and the prediction of genotypic values. Based on the mixed model methodology, the objective of this study was to investigate the comparative efficiency of eight selection indices estimated by REML/BLUP for the effective selection of superior popcorn families in the eighth intrapopulation recurrent selection cycle. We also investigated the efficiency of the inclusion of the variable "expanded popcorn volume per hectare" in the most advantageous selection of superior progenies. In total, 200 full-sib families were evaluated in two different areas in the North and Northwest regions of the State of Rio de Janeiro, Brazil. The REML/BLUP procedure resulted in higher estimated gains than those obtained with classical biometric selection index methodologies and should be incorporated into the selection of progenies. The following indices resulted in higher gains in the characteristics of greatest economic importance: the classical selection index/values attributed by trial, via REML/BLUP, and the greatest genotypic values/expanded popcorn volume per hectare, via REML. The expanded popcorn volume per hectare characteristic enabled satisfactory gains in grain yield and popping expansion; this characteristic should be considered super-trait in popcorn breeding programs.

  10. Selection of coffee progenies for resistance to nematode Meloidogyne paranaensis in infested area

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    Sonia Maria Lima Salgado

    2014-07-01

    Full Text Available The purpose of this study was to select Coffea arabica progenies for resistance to M. paranaensis in an infested coffee growing area using Henderson’s mixed model methodology. Forty-one genotypes were selected at the Coffee Active Germplasm Bank of Minas Gerais, and evaluated in regard to stem diameter, number of plagiotropic branches, reaction to the nematode, and yield per plant. There was genetic variability among the genotypes studied for all the traits evaluated, and among the populations studied for yield and reaction to the nematode, indicating possibilities for obtaining genetic gains through selection in this population. There was high rate of genotypic association between all the traits studied. Coffee plants of Timor Hybrid UFV408-01 population, and F3 progenies derived from crossing Catuaí Vermelho and Amphillo MR 2161 were the most promising in the area infested by M. paranaensis.

  11. Response to recurrent selection under small effective population size

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    Souza Jr. Cláudio Lopes de

    2000-01-01

    Full Text Available A formula was derived for the prediction of the response to recurrent selection when the effective population size (Ne is small. Usually, responses to selection have been estimated by Rs = icsigma²A/sigmaPh, where i, c, sigma²A, and sigmaPh stand for standardized selection differential, parental control, additive variance, and phenotypic standard deviation, respectively. This expression, however, was derived under the assumption of infinite population size. By introducing the effects of finite population size, the expression derived was Rs = [ic(sigma²A + deltaFD1/sigmaPh] - DFID, where deltaF, ID and D1 are the changes in the inbreeding coefficient, the inbreeding depression, and the covariance of additive and homozygous dominance effects, respectively. Thus, the predicted responses to selection based on these expressions will be smaller than those based on the standard procedures for traits with a high level of dominance such as yield. Responses to five cycles of half-sib selection were predicted for maize by both expressions, considering that 100 progenies were evaluated and 10 S1 progenies were recombined, which corresponds to Ne = 10 for each cycle. The accumulated response to selection estimated with the new expression was about 47 and 28% smaller than that based on the standard expression for yield and plant height, respectively. Thus, the expression usually used overestimates the responses to selection, which is in agreement with reported results, because it does not take into account the effective population size that is generally small in recurrent selection programs

  12. Increasing the resistance of common bean to white mold through recurrent selection

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    Monik Evelin Leite

    2016-02-01

    Full Text Available ABSTRACT White mold, caused by Sclerotinea sclerotiorum (Lib. de Bary is one of the most important diseases of the common bean (Phaseolus vulgaris L. worldwide. Physiological resistance and traits related to disease avoidance such as architecture contribute to field resistance. The aim of this study was to verify the efficiency of recurrent selection in physiological resistance to white mold, “Carioca” grain type and upright habit in common bean. Thirteen common bean lines with partial resistance to white mold were intercrossed by means of a circulant diallel table, and seven recurrent selection cycles were obtained. Of these cycles, progenies of the S0:1, S0:2 and S0:3 generations of cycles III, IV, V and VI were evaluated. The best (8 to 10 progenies of the seven cycles were also evaluated, in two experiments, one in the greenhouse and one in the field. Lattice and/or randomized block experimental designs were used. The traits evaluated were: resistance to white mold by the straw test method, growth habit and grain type. The most resistant progenies were selected based on the average score of resistance to white mold. Subsequently, they were evaluated with regard to grain type and growth habit. Recurrent selection allowed for genetic progress of about 11 % per year for white mold resistance and about 15 % per year for the plant architecture. There was no gain among cycles for grain type. Progeny selection and recurrent selection were efficient for obtaining progenies with a high level of resistance to white mold with “Carioca” grain type and upright habit.

  13. Selection of popcorn inbred lines based on performance and genealogy of S5 progenies and plants

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    José Marcelo Soriano Viana

    2009-01-01

    Full Text Available The development of hybrids is the most important objective in a corn breeding program. The objectives of thisstudy were to select and assess inbred lines, and to discuss the efficiency of four cycles of among and within inbred familyselection. Two experiments were evaluated, one with 144 S5 progenies of the popcorn population Beija-Flor and another withseven inbred lines. Based on expected gains in expansion volume, the best selective procedure was mass selection. The 14selected inbred lines should be divergent, because only two pairs were derived from the same S3 or S4 family. The analysis ofselection efficiency demonstrated that it is important to select superior plants in families with poorer performance to minimizethe loss of superior genotypes. The quality of the evaluated inbred lines was comparable to commercial populations and theyield satisfactory. The information about genealogy did not increase the inbred line selection efficiency.

  14. ARTICLE - Inbreeding depression in castor bean (Ricinus communis L. progenies

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    Milton Krieger

    2012-12-01

    Full Text Available The purpose of this study was to investigate inbreeding depression (DE in castor bean. From a population derived from the Guarani cultivar, 60 mother plants were sampled. Three types of progenies were obtained from each one: from self-pollination (AU, from crosses (CR and from open pollination (PL. Grain yield of the progenies was evaluated in two locations. There was a strong interaction of progenies x locations, which led to obtaining estimates within each location. Broad variation was observed in inbreeding depression, with mean values of 6.7% and 13.4%, comparing AU progenies with PL progenies. It was observed that the population has high potential for selecting promising inbred lines. The frequency of mother plants generating progenies with simultaneous high general combination capacity and low inbreeding depression was low. Recurrent selection will increase the occurrence of parent plants associating these two properties, which is necessary for obtaining superior synthetic varieties.

  15. Biometrical analysis and selection of tetraploid progenies of Panicum maximum using mixed model methods

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    Resende Rosangela Maria Simeão

    2004-01-01

    Full Text Available The objectives of this work were to estimate the genetic and phenotypic parameters and to predict the genetic and genotypic values of the selection candidates obtained from intraspecific crosses in Panicum maximum as well as the performance of the hybrid progeny of the existing and projected crosses. Seventy-nine intraspecific hybrids obtained from artificial crosses among five apomictic and three sexual autotetraploid individuals were evaluated in a clonal test with two replications and ten plants per plot. Green matter yield, total and leaf dry matter yields and leaf percentage were evaluated in five cuts per year during three years. Genetic parameters were estimated and breeding and genotypic values were predicted using the restricted maximum likelihood/best linear unbiased prediction procedure (REML/BLUP. The dominant genetic variance was estimated by adjusting the effect of full-sib families. Low magnitude individual narrow sense heritabilities (0.02-0.05, individual broad sense heritabilities (0.14-0.20 and repeatability measured on an individual basis (0.15-0.21 were obtained. Dominance effects for all evaluated characteristics indicated that breeding strategies that explore heterosis must be adopted. Less than 5% increase in the parameter repeatability was obtained for a three-year evaluation period and may be the criterion to determine the maximum number of years of evaluation to be adopted, without compromising gain per cycle of selection. The identification of hybrid candidates for future cultivars and of those that can be incorporated into the breeding program was based on the genotypic and breeding values, respectively. The prediction of the performance of the hybrid progeny, based on the breeding values of the progenitors, permitted the identification of the best crosses and indicated the best parents to use in crosses.

  16. Spatial dependence in experiments of progeny selection for bean ( Phaseolus vulgaris L. yield

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    Michele Jorge da Silva

    Full Text Available ABSTRACT In field experiments, it is often assumed that errors are statistically independent, but not always this condition is met, compromising the results. An inappropriate choice of the analytical model can compromise the efficiency of breeding programs in preventing unpromising genotypes from being selected and maintained in the next selection cycles resulting in waste of time and resources. The objective of this study was to evaluate the spatial dependence of errors in experiments evaluating grain yield of bean progenies using analyses in lattice and randomized blocks. And also evaluate the efficiency of geostatistical models to describe the structure of spatial variability of errors. The data used in this study derived from experiments arranged in the lattice design and analyzed as lattice or as randomized blocks. The Durbin-Watson test was used to verify the existence of spatial autocorrelation. The theoretical semivariogram was fitted using geostatistical models (exponential, spherical and Gaussian to describe the spatial variability of errors. The likelihood ratio test was applied to assess the significance of the geostatistical model parameters. Of the eight experiments evaluated, five had moderate spatial dependence for the randomized blocks analysis and one for both analyses, in lattice and randomized blocks. The area of the experiments was not a determinant factor of the spatial dependence. The spherical, exponential and Gaussian geostatistical models with nugget effect were suitable to represent the spatial structure in the randomized block analysis. The analysis in lattice was efficient to ensure the independence of errors.

  17. COMPARISON BETWEEN AND WITHIN FAMILY SELECTIONS WITH THE COMBINED SELECTION IN PROGENY TESTS OF Eucalyptus grandis

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    Ildeu Soares Martins

    2005-03-01

    Full Text Available This study used six experiments involving half-sub families of Eucalyptus grandis, which were carried out byCelulose Nipo Brasileira S. A. (CENIBRA at six locations. Data on circumference at breast height and plant height weremeasured. Selection methods among and within families were compared with combined selection. The combined selection wassuperior to selection among and within families due to higher estimates of genetic gains, and also for selecting a larger numberof families for recombination.

  18. Selection and genetic gains for juvenile traits in progenies of Hevea in São Paulo State, Brazil

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    Paulo de Souza Gonçalves

    2004-01-01

    Full Text Available Five yield traits were investigated in three-year-old progenies from open-pollinated rubber trees [Hevea brasiliensis (Willd. ex Adr. de Juss Muell.-Arg.]. Twenty progenies were evaluated in a randomized, complete block design replicated three times using 10 plants per linear plot at the North Central Experimental Station in Pindorama, São Paulo State, Brazil. The characters evaluated included the average yield of rubber, growth vigor, bark thickness, total number of latex vessel rings and latex vessel size. Highly significant (p were 37%, 35%, 69%, 10% and 16%, respectively. Significant positive genotypic and phenotypic correlations were found between the yield of rubber and growth vigor (r g = 0.73, r p = 0.70, bark thickness (r g = 0.70**, r p = 0.75** and the total number of latex vessel rings (r g = 0.64, r p = 0.80. There was no relationship between yield and latex vessel size, growth vigor or total number of latex vessel rings. Based on these data, selecting the best two out of 20 progenies would result in a genetic gain of 12.3% and 6.8% for yield of rubber and growth vigor, respectively. The two best individual ortets within each progeny would result in a genetic gain of 27.7% and 9.1%, with a total gain of 40% and 16% for these two traits, respectively.

  19. Recurrent selection in inbred popcorn families

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    Daros Máskio

    2004-01-01

    Full Text Available Although much appreciated in Brazil, commercial popcorn is currently cropped on a fairly small scale. A number of problems need to be solved to increase production, notably the obtaintion of seeds with good agronomic traits and good culinary characteristics. With the objective of developing superior genotypes in popcorn, a second cycle of intrapopulation recurrent selection based on inbred S1 families was carried out. From the first cycle of selection over the UNB-2U population, 222 S1 families were obtained, which were then divided into six sets and evaluated in a randomized complete block design with two replications within the sets. Experiments were carried out in two Brazilian localities. The analysis of variance revealed environmental effects for all evaluated traits, except popping and stand, showing that, for most traits, these environments affected genotype behavior in different ways. In addition, the set as source of variation was significant for most of the evaluated traits, indicating that dividing the families into sets was an efficient strategy. Genotype-by-environment interaction was detected for most traits, except popping expansion and stand. Differences among genotypes were also detected (1% F-test, making viable the proposition of using the genetic variability in the popcorn population as a basis for future recurrent selection cycles. Superior families were selected using the Smith and Hazel classic index, with predicted genetic gains of 17.8% for popping expansion and 26.95% for yield.

  20. Agronomic performance of 27 cocoa progenies and plant selection based on productivity, self-compatibility and disease resistance

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    Milton Macoto Yamada

    2013-08-01

    Full Text Available The objective of the present work was to evaluate 27 progenies of cocoa crosses considering the agronomic traits and select F1 plants within superior crosses. The experiment was installed in March 2005, in the Experimental Station Joaquim Bahiana (ESJOB, in Itajuipe, Bahia. The area of the experiment is of approximately 3 ha, with a total of 3240 plants. Thirteen evaluations of vegetative brooms, five of cushion brooms and 15 of number of pods per plant were accomplished. Thirty pollinations were made for each selected plant to test for self-compatibility. The production, based on the number of pods per plant, and resistance to witches´ broom indicated CEPEC 94 x CCN 10, RB 39 x CCN 51 and CCN 10 x VB 1151 as superior progenies. All selections tested were self-compatible. The analyses of progenies and individual tree data, associated to visual field observations, allowed the selection of 17 plants which were included in a network of regional tests to determine the phenotypic stability.

  1. Effect of Recurrent Selection on Drought Tolerance and Related Morpho-Physiological Traits in Bread Wheat.

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    Ramya P

    Full Text Available Drought is one of the major abiotic stresses affecting wheat yield. A recurrent selection program was conducted to improve the drought tolerance and yield of bread wheat using drought tolerant advanced breeding lines from a drought tolerant x susceptible cross (HI 1500 x HUW 510. The parental lines were evaluated for yield, biomass and harvest index (HI in addition to the drought adaptive traits like Canopy Temperature (CT, chlorophyll content and Normalized Difference Vegetative Index (NDVI. After three rounds of recurrent selection, the half sib progenies exhibited a marked reduction in CT, chlorophyll content and biomass; whereas improvement was noted for yield, HI and NDVI. Drought tolerance of the half-sib population appeared enhanced as indicated by drought indices and grain yield. Compared to base population, half-sibs showed better HI, grain filling and a significant (17.1% increase in grain yield under water stress conditions. Cooler canopies and increased early vigour might have contributed to drought tolerance. A favourable combination of gibberellin sensitive and insensitive Rhtalleles was observed in the recombinant progenies. However, increased yield under water stress had a negative trade off in reduction of biomass. The study also identified potential lines with high yield and drought tolerance for subsequent varietal development for water limited areas.

  2. Estimates for Genetic Variance Components in Reciprocal Recurrent Selection in Populations Derived from Maize Single-Cross Hybrids

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    Matheus Costa dos Reis

    2014-01-01

    Full Text Available This study was carried out to obtain the estimates of genetic variance and covariance components related to intra- and interpopulation in the original populations (C0 and in the third cycle (C3 of reciprocal recurrent selection (RRS which allows breeders to define the best breeding strategy. For that purpose, the half-sib progenies of intrapopulation (P11 and P22 and interpopulation (P12 and P21 from populations 1 and 2 derived from single-cross hybrids in the 0 and 3 cycles of the reciprocal recurrent selection program were used. The intra- and interpopulation progenies were evaluated in a 10×10 triple lattice design in two separate locations. The data for unhusked ear weight (ear weight without husk and plant height were collected. All genetic variance and covariance components were estimated from the expected mean squares. The breakdown of additive variance into intrapopulation and interpopulation additive deviations (στ2 and the covariance between these and their intrapopulation additive effects (CovAτ found predominance of the dominance effect for unhusked ear weight. Plant height for these components shows that the intrapopulation additive effect explains most of the variation. Estimates for intrapopulation and interpopulation additive genetic variances confirm that populations derived from single-cross hybrids have potential for recurrent selection programs.

  3. Selection arena in Aspergillus nidulans : early progeny choice in a filamentous fungus = Selectie arena in Aspergillus nidulans : vroege selectie van nakomelingen in een filamenteuse schimmel

    NARCIS (Netherlands)

    Bruggeman, J.

    2003-01-01

    Keywords:selection arena, progeny choice, Aspergillus nidulans , fungus, spores, ascospore, conidiospore, asexual, sexual, fruiting body, cleistothecium, zygote, dikaryon, self-sterility, self-fertility, mutation accumulation, fitness,

  4. Response of locally adapted pearl millet populations to s1 progeny ...

    African Journals Online (AJOL)

    Response of locally adapted pearl millet populations to s1 progeny recurrent selection for ... African Crop Science Journal ... This led to grain yield of 1,047 from 611 kg ha-1 in Lam population and 943 from 693 kg ha-1 in Omoda population.

  5. Genetic progress in the UNB-2U population of popcorn under recurrent selection in Rio de Janeiro, Brazil.

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    Ribeiro, R M; do Amaral Júnior, A T; Gonçalves, L S A; Candido, L S; Silva, T R C; Pena, G F

    2012-05-15

    As part of the Universidade Estadual do Norte Fluminense recurrent selection program of popcorn, we evaluated full-sib families of the sixth cycle of recurrent selection and estimated genetic progress for grain yield and expansion capacity. We assessed 200 full-sib families for 10 agronomic traits, in a randomized block design, with two replications within sets in two environments: Campos dos Goytacazes and Itaocara, in the State of Rio de Janeiro, Brazil. There were significant differences for families/"sets" for all traits, indicating genetic variability that could be exploited in future cycles. In the selection of superior progenies, the Mulamba and Mock index gave the best gains for popping expansion (PE) and grain yield (GY), with values of 10.97 and 15.30%, respectively, using random economic weights. By comparing the evolution of the means obtained for PE and GY in the cycles C(0), C(1), C(2), C(3), C(4), C(5), and predicted for C(6), a steady increase was observed for both PE and GY, with the addition of 1.71 mL/g (R(2) = 0.93) and 192.87 kg/ha (R(2) = 0.88), respectively, in each cycle. Given the good performance of this popcorn population in successive cycles of intrapopulation recurrent selection, we expect that a productive variety with high expansion capacity will soon be available for producers in the north and northwest regions of Rio de Janeiro State, Brazil.

  6. Genetic gains in the UENF-14 popcorn population with recurrent selection.

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    Freitas, I L J; do Amaral Júnior, A T; Freitas, S P; Cabral, P D S; Ribeiro, R M; Gonçalves, L S A

    2014-01-21

    The popcorn breeding program of Universidade Estadual do Norte Fluminense Darcy Ribeiro aims to provide farmers a cultivar with desirable agronomic traits, particularly with respect to grain yield (GY) and popping expansion (PE). We evaluated full-sib families from the seventh cycle of recurrent selection and estimated the genetic progress with respect to GY and PE. Eight traits were evaluated in 200 full-sib families that were randomized into blocks with two replicates per set in two contrasting environments, Campos dos Goytacazes and Itaocara, located in north and northwest Rio de Janeiro State, respectively. There were significant differences between sets in families with respect to all traits evaluated, which indicates genetic variability that may be explored in future cycles. Using random economic weights in the selection of superior progenies, the Mulamba and Mock index showed gains for PE and GY of 5.11 and 7.78%, respectively. Significant PE and GY increases were found when comparing the evolution of mean values of these two parameters that were assessed at cycles C₀-C₆ and predicted for C₇. Thus, an advanced-cycle popcorn cultivar with genotypic superiority for the main traits of economic interest can be made available to farmers in Rio de Janeiro State.

  7. Use of recurrent selection of early flowering in late maize synthetic population. Results of second cycle of breeding

    Directory of Open Access Journals (Sweden)

    N. Petrovska

    2017-03-01

    Full Text Available Abstract. During the period 2012 – 2014, a second cycle of recurrent selection of early flowering in a synthetic maize population “Exotic-07” was conducted 2 and finished on the experimental field of Maize Research institute – Knezha. The experiments are carried out by a block method, on a test plot of 10 m , with three replications, and the respective for the region agricultural equipment. Twenty-three progeny from the first and second cycle, the source and improved exotic maize populations, as well as their testcrosses with the lines ХМ 4416 and PAU 1617 are tested. A phenotypic cycle assumes a leading position in terms of dates of plant silking as the forms of the earliest flowering are used as pollen in the population. The selected early flowering forms are sown for inbreeding and forming an improved maize population. As a direct result of the work of improvement, progeny with a period of days until silking averagely shorter with 5 days and grain moisture lowered by 1.1% are obtained. The aim of this study is to point out inbred lines with a shorter vegetative period and use them as parental forms for obtaining high-yielding mid-late maize hybrids.

  8. Improvement of a popcorn population using selection indexes from a fourth cycle of recurrent selection program carried out in two different environments.

    Science.gov (United States)

    Amaral Júnior, A T; Freitas Júnior, S P; Rangel, R M; Pena, G F; Ribeiro, R M; Morais, R C; Schuelter, A R

    2010-03-02

    We estimated genetic gains for popcorn varieties using selection indexes in a fourth cycle of intrapopulation recurrent selection developed in the campus of the Universidade Estadual do Norte Fluminense. Two hundred full-sib families were obtained from the popcorn population UNB-2U of the third recurrent selection cycle. The progenies were evaluated in a randomized block design with two replications at sites in two different environments: the Colégio Estadual Agrícola Antônio Sarlo, in Campos dos Goytacazes, and the Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (PESAGRO-RIO), in Itaocara, both in the State of Rio de Janeiro. There were significant differences between families within sets in all traits, indicating genetic variability that could be exploited in future cycles. Thirty full-sib families were selected to continue the program. The selection indexes used to predict the gains were those of Mulamba and Mock, Smith and Hazel. The best results were obtained with the Mulamba and Mock index, which allowed the prediction of negative gains for the traits number of diseased ears and ears attacked by pests, number of broken plants and lodging, as well as ears with poor husk cover. It also provided higher gains for popping expansion and grain yield than with the other indexes, giving values of 10.55 and 8.50%, respectively, based on tentatively assigned random weights.

  9. Shoulder Dysfunction after Selective Neck Dissection in Recurrent Nasopharyngeal Carcinoma.

    Science.gov (United States)

    Chan, Jimmy Yu Wai; Wong, Stanley Thian Sze; Chan, Richie Chiu Lung; Wei, William Ignace

    2015-09-01

    To investigate the incidence of occult nodal metastasis and severity of shoulder dysfunction after selective neck dissection (SND) for recurrent nasopharyngeal carcinoma (NPC) with N0 status. Prospective, single-group, pre/post test design. Academic medical center. Between 1998 and 2012, 46 patients who had recurrent NPC and N0 status were recruited. They subsequently received salvage nasopharyngectomy and SND, removing ipsilateral level I to III and V lymphatics. The incidence of occult nodal metastasis was noted. All patients underwent standardized physiotherapy after surgery. Postoperative shoulder function was measured using the Disability of Arm, Shoulder, and Hand (DASH) questionnaire. The incidence of microscopic nodal metastasis was 15.2%. For first year posttreatment, the mean DASH score was 44.2. With time, there was no improvement in shoulder function despite targeted physiotherapy (P = .09), and the second postoperative year mean DASH score was 46.3. The degree of daily activity affected was rated as moderate to very limited, and 30% of the patients had at least moderate shoulder pain at rest. Shoulder dysfunction after SND for recurrent NPC is significant and persistent. Given the low incidence of microscopic nodal metastasis in such circumstances, routine SND is not recommended. © American Academy of Otolaryngology—Head and Neck Surgery Foundation 2015.

  10. Isoenzymatic variability in tropical maize populations under reciprocal recurrent selection

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    Pinto Luciana Rossini

    2003-01-01

    Full Text Available Maize (Zea mays L. is one of the crops in which the genetic variability has been extensively studied at isoenzymatic loci. The genetic variability of the maize populations BR-105 and BR-106, and the synthetics IG-3 and IG-4, obtained after one cycle of a high-intensity reciprocal recurrent selection (RRS, was investigated at seven isoenzymatic loci. A total of twenty alleles were identified, and most of the private alleles were found in the BR-106 population. One cycle of reciprocal recurrent selection (RRS caused reductions of 12% in the number of alleles in both populations. Changes in allele frequencies were also observed between populations and synthetics, mainly for the Est 2 locus. Populations presented similar values for the number of alleles per locus, percentage of polymorphic loci, and observed and expected heterozygosities. A decrease of the genetic variation values was observed for the synthetics as a consequence of genetic drift effects and reduction of the effective population sizes. The distribution of the genetic diversity within and between populations revealed that most of the diversity was maintained within them, i.e. BR-105 x BR-106 (G ST = 3.5% and IG-3 x IG-4 (G ST = 4.0%. The genetic distances between populations and synthetics increased approximately 21%. An increase in the genetic divergence between the populations occurred without limiting new selection procedures.

  11. Recurrent selection of popcorn composites UEMCO1 and UEM-CO2 based on selection indices

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    Rafael Augusto Vieira

    2017-06-01

    Full Text Available Selection indices were applied to data sets of 169 half-sib families of the popcorn composites UEM-Co1 and UEM-Co2 in four cycles of recurrent selection. From 2005 to 2008, the experiments were arranged in a 13 by 13 lattice square design, with two replications per cycle and composite. Genetic gains for popping expansion (PE and grain yield (GY were estimated based on several selection indices and truncation selection. The magnitude and balance of gains estimated for each trait by the indices were compared by an auxiliary statistical value (Ci. This value Ci consists of an arbitrary value, resulting from differences between the gains estimated for n traits by truncation selection and by index i. The indices of Subandi and Mulamba and Mock were the most promising to estimate high and balanced genetic gains for PE and GY in recurrent selection of half-sib popcorn families.

  12. Selection index and molecular markers in reciprocal recurrent selection in maize

    Directory of Open Access Journals (Sweden)

    Flávio Dessaune Tardin

    2007-01-01

    Full Text Available Reciprocal recurrent selection among full-sib families is one of the outstanding methodologies of maizeimprovement, since gains are possible in the per se and/or in cross populations. Here the selection index proposed by Smithand Hazel was used in the cited methodology; besides, an additional phase involving RAPD markers was introduced topreserve the genetic variability of the selected genotypes and identify contaminants before their recombination. Multivariatetechniques of grouping and discriminant analyses were used for this purpose, to assure the continuance of the improvementprogram and amplify the genetic distance between the populations Cimmyt and Piranão, which were used for an intervarietalhybrid. The molecular technique proved useful to identify contaminants and helpful in the choice of the genotypes to berecombined to maximize heterosis among populations. The technique can be included in recurrent selection programs, mainlythose that target the development of hybrids.

  13. Genetic variance and covariance components related to intra- and interpopulation recurrent selection in maize (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Arias Carlos Alberto Arrabal

    1998-01-01

    Full Text Available New genetic variance and covariance components related to intra- and interpopulational recurrent selection methods have been theoretically developed by Souza Jr. (Rev. Bras. Genet. 16: 91-105, 1993 to explain the failure of these methods to concomitantly develop hybrid and per se populations. Intra- and interpopulation half-sib progenies of 100 genotypes were sampled from maize (Zea mays L. populations BR-106 and BR-105 to estimate variance and covariance components and to compare the expected responses to reciprocal (RRS, intrapopulational (HSS, and modified (MRS recurrent selection in interpopulation hybrid, populations per se, and to determine heterosis. Four sets of 100 progenies, two intra- and two interpopulational, were evaluated in partially balanced 10 x 10 lattices arranged in split-blocks with two replications in two years (1991/92 and 1992/93 and two locations in Piracicaba, SP. Data for ear weight, plant and ear height, and ear by plant height ratio were recorded. Populations and interpopulation crosses were high yielding and showed high breeding potential for production of hybrids from inbred lines. Mid parent and the highest parent heterosis were relatively high, but lower than values reported for these populations under other environmental conditions. Additive variance estimates of populations per se and interpopulation crosses confirmed the high potential of these materials. The magnitude of the variance estimates for the deviations from intra- and interpopulation additive effects ( for BR-106 and for BR-105 and covariance between additive effects with these deviations ( for BR-106 and for BR-105 indicated that these new components can significantly influence the effectiveness of breeding methods. Genetic component estimates for BR-105 had relatively small errors, with negative for all traits. Estimates of and had relatively larger errors for BR-106. The MRS method was more effective than the RRS and HSS methods in producing

  14. New strategy for evaluating grain cooking quality of progenies in dry bean breeding programs

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    Bruna Line Carvalho

    2017-04-01

    Full Text Available The methodology available for evaluating the cooking quality of dry beans is impractical for assessing a large number of progenies. The aims of this study were to propose a new strategy for evaluating cooking quality of grains and to estimate genetic and phenotypic parameters using a selection index. A total of 256 progenies of the 13thcycle of a recurrent selection program were evaluated at three locations for yield, grain type, and cooked grains. Samples of grains from each progeny were placing in a cooker and the percentage of cooked grains was assessed. The new strategy for evaluating cooking quality was efficient because it allowed a nine-fold increase in the number of progenies evaluated per unit time in comparison to available methods. The absence of association between grain yield and percentage of cooked grains or grain type indicated that it is possible to select high yielding lines with excellent grain aspect and good cooking properties using a selection index.

  15. Economic evaluation of progeny-testing and genomic selection schemes for small-sized nucleus dairy cattle breeding programs in developing countries.

    Science.gov (United States)

    Kariuki, C M; Brascamp, E W; Komen, H; Kahi, A K; van Arendonk, J A M

    2017-03-01

    In developing countries minimal and erratic performance and pedigree recording impede implementation of large-sized breeding programs. Small-sized nucleus programs offer an alternative but rely on their economic performance for their viability. We investigated the economic performance of 2 alternative small-sized dairy nucleus programs [i.e., progeny testing (PT) and genomic selection (GS)] over a 20-yr investment period. The nucleus was made up of 453 male and 360 female animals distributed in 8 non-overlapping age classes. Each year 10 active sires and 100 elite dams were selected. Populations of commercial recorded cows (CRC) of sizes 12,592 and 25,184 were used to produce test daughters in PT or to create a reference population in GS, respectively. Economic performance was defined as gross margins, calculated as discounted revenues minus discounted costs following a single generation of selection. Revenues were calculated as cumulative discounted expressions (CDE, kg) × 0.32 (€/kg of milk) × 100,000 (size commercial population). Genetic superiorities, deterministically simulated using pseudo-BLUP index and CDE, were determined using gene flow. Costs were for one generation of selection. Results show that GS schemes had higher cumulated genetic gain in the commercial cow population and higher gross margins compared with PT schemes. Gross margins were between 3.2- and 5.2-fold higher for GS, depending on size of the CRC population. The increase in gross margin was mostly due to a decreased generation interval and lower running costs in GS schemes. In PT schemes many bulls are culled before selection. We therefore also compared 2 schemes in which semen was stored instead of keeping live bulls. As expected, semen storage resulted in an increase in gross margins in PT schemes, but gross margins remained lower than those of GS schemes. We conclude that implementation of small-sized GS breeding schemes can be economically viable for developing countries. The

  16. Recurrent connections form a phase-locking neuronal tuner for frequency-dependent selective communication.

    Science.gov (United States)

    Shin, Dongkwan; Cho, Kwang-Hyun

    2013-01-01

    The brain requires task-dependent interregional coherence of information flow in the anatomically connected neural network. However, it is still unclear how a neuronal group can flexibly select its communication target. In this study, we revealed a hidden routing mechanism on the basis of recurrent connections. Our simulation results based on the spike response model show that recurrent connections between excitatory and inhibitory neurons modulate the resonant frequency of a local neuronal group, and that this modulation enables a neuronal group to receive selective information by filtering a preferred frequency component. We also found that the recurrent connection facilitates the successful routing of any necessary information flow between neuronal groups through frequency-dependent resonance of synchronized oscillations. Taken together, these results suggest that recurrent connections act as a phase-locking neuronal tuner which determines the resonant frequency of a local group and thereby controls the preferential routing of incoming signals.

  17. Selective attention and fear of cancer recurrence in breast cancer survivors.

    Science.gov (United States)

    Custers, J A E; Becker, E S; Gielissen, M F M; Van Laarhoven, H W M; Rinck, M; Prins, J B

    2015-02-01

    Anxious people show an attentional bias towards threatening information. It was investigated whether an attentional bias exists for cancer-related stimuli in breast cancer survivors and if different levels of fear of cancer recurrence would lead to different patterns of selective attention. Breast cancer survivors with high (n = 35) and low (n = 32) fear of cancer recurrence were compared to 40 healthy female hospital employees. Specificity of attentional biases was investigated using a modified Emotional Stroop Task. Self-report measures were used to assess depression and anxiety, feelings of fatigue, and experienced traumas. Compared to control participants, breast cancer survivors with both high and low levels of fear of cancer recurrence showed increased interference for cancer-related words, but not for other word types. The findings suggest a specific attentional bias for cancer-related words in breast cancer survivors that is independent of level of fear of cancer recurrence.

  18. Genetic gains in popcorn by full-sib recurrent selection

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    Antonio Teixeira do Amaral Júnior

    2009-01-01

    Full Text Available The purpose of this study was to investigate the genetic progress in the fourth selection cycle of full-sibpopcorn families. Two hundred families were evaluated for six agronomic traits, including grain yield (GY and poppingexpansion (PE, in a random block design with two replications, in sets in two different environments: Campos dos Goytacazesand Itaocara, in the State of Rio de Janeiro. There were significant differences among families within sets in all traits,indicating the presence of genetic variability to be exploited in the future cycles. The family by environment in the sets for GYand PE was significant but simple, indicating the possibility of conducting a single breeding program for both locations. Therelease of the improved variety for producers of the Northern and Northwestern regions of the State of Rio de Janeiro isexpected after the fifth cycle.

  19. Selective Attention and Fear of Cancer Recurrence in Breast Cancer Survivors

    NARCIS (Netherlands)

    Custers, J. A. E.; Becker, E. S.; Gielissen, M. F. M.; van Laarhoven, H. W. M.; Rinck, M.; Prins, J. B.

    2015-01-01

    Anxious people show an attentional bias towards threatening information. It was investigated whether an attentional bias exists for cancer-related stimuli in breast cancer survivors and if different levels of fear of cancer recurrence would lead to different patterns of selective attention. Breast

  20. Genetic Variation Among Open-Pollinated Progeny of Eastern Cottonwood

    Science.gov (United States)

    R. E. Farmer

    1970-01-01

    Improvement programs in eastern cottonwood (Populus deltoides Bartr.) are most frequently designed to produce genetically superior clones for direct commercial use. This paper describes a progeny test to assess genetic variability on which selection might be based.

  1. Eficiência na seleção de progênies de cafeeiro avaliadas em Minas Gerais Selection efficiency of coffee progenies evaluated in the State of Minas Gerais, Brazil

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    Marcelo Frota Pinto

    2012-01-01

    Full Text Available O presente trabalho teve como objetivo verificar a eficiência de seleção das progênies de cafeeiros derivadas do cruzamento entre Catuaí com Icatu e com Híbrido de Timor. Foram avaliadas 18 progênies desenvolvidas pelo Programa de Melhoramento Genético da Epamig/UFLA/UFV. Os ensaios foram instalados em dezembro de 2005, nas cidades de Lavras, Campos Altos e Patrocínio, em Minas Gerais. As características analisadas foram as seguintes: produtividade, grãos retidos em peneira "17 acima" e frutos cereja em porcentagem. Para verificar a eficiência de seleção, foi aplicado o índice de coincidência segundo métodos de HAMBLIN and ZIMMERMANN (1986 e a magnitude da interação progênies x ambientes e sua decomposição em parte simples e complexa utilizando método de CRUZ e CASTOLDI (1991. A predominância da parte complexa em relação à simples na interação das progênies x ambientes confirma a baixa ou não coincidência das progênies nos diferentes ambientes, dificultando uma recomendação generalizada para as diferentes regiões cafeeiras do Estado.The present work had the aim of verifying the selection efficiency of coffee plants originated from the crossing between Catuaí and Icatu with Timor Hybrid. Eighteen progenies developed by the Program of Genetic Improvement of Epamig/UFLA/UFV were evaluated. Coffee plants were planted in December 2005 in the following counties: Lavras, Campos Altos and Patrocínio, in the State of Minas Gerais. The characteristics analyzed were: productivity, grain size and proportion of cherry fruit. In order to verify the efficiency of the selection, the index of coincidence was applied according to the methodology of HAMBLIN and ZIMMERMANN (1986, as well as the magnitude of the interaction progenies x environments and its decomposition in simple and complex parts using the methodology of CRUZ and CASTOLDI (1991. The predominance of the complex part in relation to the simple one in the

  2. Bayesian analysis of quantitative traits in popcorn (Zea mays L. through four cycles of recurrent selection

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    Antonio Teixeira do Amaral Junior

    2016-10-01

    Full Text Available A Bayesian analysis of genetic parameters for quantitative traits was carried out in full-sib families of popcorn through four cycles (C5, C6, C7, and C8 of recurrent selection. The parameters of Bayesian model were estimated using the independence chain algorithm. Grain yield and plant height were found to be moderately heritable ranging from 0.25 to 0.48 and 0.45 to 0.58, respectively. The highest heritability estimates were found for popping expansion volume at C5 ($ \\hat{h}_{C_5}^{2} $ = 0.71, and C7 ($ \\hat{h}_{C_7}^{2} $ = 0.63. The response of recurrent selection was 30.5 and 32.3% for grain yield and popping expansion volume, respectively. In C8, predicted genetic advances (from the Bayesian prediction of genotypic values were 4.8, 4.3, and 2.9% for plant height, grain yield, and popping expansion volume, respectively. The significant genetic variability among popcorn families in successive cycles of recurrent selection has sustained continued genetic progress in breeding for the traits of interest.

  3. Prediction of biochemical recurrence after radical prostatectomy. New tool for selecting candidates for adjuvant radiation therapy.

    Science.gov (United States)

    Herranz-Amo, F; Molina-Escudero, R; Ogaya-Pinies, G; Ramírez-Martín, D; Verdú-Tartajo, F; Hernández-Fernández, C

    2016-03-01

    To design a risk summation to select patients for adjuvant radiation therapy after prostatectomy. A retrospective study was conducted on 629 patients with localised prostate cancer (pN0-pNx) who were treated with prostatectomy and with a prostate-specific antigen (PSA) value 0.4ng/mL. A multivariate Cox regression analysis was performed. A score (0-2) was assigned according to the hazard ratio of the significant variables. The score summation defined the risk summation. A total of 19.7% of the patients were pT3, 24.2% had a Gleason score ≥ 8, and 26.3% had positive surgical margins. The median follow-up was 82 months. Some 26.6% of the patients experienced biochemical recurrence. The identified prognostic variables independent of biochemical recurrence were a Gleason score =7 (4+3) (HR, 2.01; P=.008), a Gleason score ≥ 8 (HR, 3.07; P 50% survival free of biochemical recurrence at 5 and 8 years. In contrast, the patients with a risk summation ≥ 3 had <44% survival free of biochemical recurrence. The patients with a risk summation ≤ 2 did not benefit from adjuvant radiation therapy, while the patients with a risk summation ≥ 3 might benefit from adjuvant radiation therapy. Copyright © 2015 AEU. Publicado por Elsevier España, S.L.U. All rights reserved.

  4. Genetic drift and selection effects of modified recurrent full-sib selection programs in two F2 populations of European flint maize.

    Science.gov (United States)

    Flachenecker, C; Frisch, M; Falke, K C; Melchinger, A E

    2006-10-01

    Selection response of a modified recurrent full-sib (FS) selection scheme conducted in two European flint F(2) maize (Zea mays L.) populations was re-evaluated. Our objectives were to (1) determine the selection response for per se and testcross performance in both populations and (2) separate genetic effects due to selection from those due to random genetic drift. Modified recurrent FS selection was conducted at three locations using an effective population size N(e) = 32 and a selection rate of 25% for a selection index, based on grain yield and grain moisture. Recombination was performed according to a pseudo-factorial mating scheme. Selection response was assessed using a population diallel including the source population and advanced selection cycles, as well as testcrosses with unrelated inbred line testers and the parental F(1) generation. Selection response per cycle was significant for grain yield and grain moisture in both populations. Effects of random genetic drift caused only a small reduction in the selection response. No significant selection response was observed for testcrosses, suggesting that for heterotic traits, such as grain yield, a high frequency of favorable alleles in the elite tester masked the effects of genes segregating in the populations. We conclude that our modified recurrent FS selection is an alternative to other commonly applied intrapopulation recurrent selection schemes, and some of its features may also be useful for increasing the efficiency of interpopulation recurrent selection programs.

  5. Impact of FDG PET in optimizing patient selection for cytoreductive surgery in recurrent ovarian cancer

    Energy Technology Data Exchange (ETDEWEB)

    Ebina, Yasuhiko; Yamada, Hideto [Kobe University Graduate School of Medicine, Department of Obstetrics and Gynecology, Kobe (Japan); Watari, Hidemichi; Kaneuchi, Masanori; Takeda, Mahito; Hosaka, Masayoshi; Kudo, Masataka; Sakuragi, Noriaki [Hokkaido University Graduate School of Medicine, Department of Gynecology, Sapporo (Japan)

    2014-03-15

    To investigate the impact of PET and PET/CT scanning on decision-making in management planning and to identify the optimal setting for selecting candidates for surgery in suspicious recurrent ovarian cancer. A retrospective chart review was performed in patients with possible recurrent ovarian cancer after primary optimal cytoreduction and taxane/carboplatin chemotherapy who had undergone FDG PET or FDG PET/CT scans from July 2002 to August 2008 to help make treatment decisions. The analysis included 44 patients who had undergone a total of 89 PET scans. The positive PET scans were classified as follows. (1) localized (one or two localized sites of FDG uptake), (2) multiple (three or more sites of FDG uptake), (3) diffuse (extensive low-grade activity outlining serosal and peritoneal surfaces). Of the 89 PET scans, 52 (58.4 %) led to a change in management plan. The total number of patients in whom cytoreductive surgery was selected as the treatment of choice increased from 12 to 35. Miliary disseminated disease, which was not detected by PET scan, was found in 22.2 % of those receiving surgery. Miliary disseminated disease was detected in 6 of the 12 patients with recurrent disease whose treatment-free interval (TFI) was <12 months, whereas none of those with a TFI of ≥12 months had such disease (P = 0.0031). PET or PET/CT is useful for selecting candidates for cytoreductive surgery among patients with recurrent ovarian cancer. To avoid surgical attempts in those with miliary dissemination, patients with a TFI of ≥12 months are the best candidates for cytoreductive surgery. (orig.)

  6. Recurrent twin-twin transfusion syndrome after selective fetoscopic laser photocoagulation: a systematic review of the literature.

    LENUS (Irish Health Repository)

    Walsh, C A

    2012-11-01

    Selective fetoscopic laser photocoagulation (SFLP) is now the treatment of choice for twin-twin transfusion syndrome (TTTS). The incidence of recurrent TTTS following SFLP has been inconsistently reported across different studies. We performed a systematic review of TTTS recurrence following SFLP.

  7. Seleção de progênies de maracujazeiro-amarelo (Passiflora edulis f. flavicarpa quanto à qualidade de frutos Selection of progenies of yellow passion fruit (Passiflora edulis f. flavicarpa to fruit quality

    Directory of Open Access Journals (Sweden)

    Walnice Maria Oliveira do Nascimento

    2003-04-01

    desirable characteristics for concentrate juice industry and "in natura" fruit. The study was carried out in 20 progenies from free pollination originating of a population formed by the mixture of seeds from plants selected in the second cycle. Ten fruits were collected by plant to be analyzed based on in the following characteristics: mean weight of fruits; fruit length; thickness shell; percentage of pulp; number of seeds; total soluble solids, titratable acidity (TA; pH and relationship TSS/TA. The data were analyzed through simple statistics, involving averages, standard deviation and variation coefficient. The progenies CPATU fine-sheel (CCF showed variation for most of the characteristics. The progenies CCF-001, CCF-074, CCF-212, CCF-395 and CCF-430 are suitable for the "in natura" fruit market, while the progenies CCF-192, CCF-281, CCF-391 and CCF-505 are suitable for concentrate juice industry.

  8. Optimal Feature Space Selection in Detecting Epileptic Seizure based on Recurrent Quantification Analysis and Genetic Algorithm

    Directory of Open Access Journals (Sweden)

    Saleh LAshkari

    2016-06-01

    Full Text Available Selecting optimal features based on nature of the phenomenon and high discriminant ability is very important in the data classification problems. Since it doesn't require any assumption about stationary condition and size of the signal and the noise in Recurrent Quantification Analysis (RQA, it may be useful for epileptic seizure Detection. In this study, RQA was used to discriminate ictal EEG from the normal EEG where optimal features selected by combination of algorithm genetic and Bayesian Classifier. Recurrence plots of hundred samples in each two categories were obtained with five distance norms in this study: Euclidean, Maximum, Minimum, Normalized and Fixed Norm. In order to choose optimal threshold for each norm, ten threshold of ε was generated and then the best feature space was selected by genetic algorithm in combination with a bayesian classifier. The results shown that proposed method is capable of discriminating the ictal EEG from the normal EEG where for Minimum norm and 0.1˂ε˂1, accuracy was 100%. In addition, the sensitivity of proposed framework to the ε and the distance norm parameters was low. The optimal feature presented in this study is Trans which it was selected in most feature spaces with high accuracy.

  9. Family versus individual plant selection for stem borer ( Eldana ...

    African Journals Online (AJOL)

    Parents selected from breeding programmes where E. saccharina is endemic consistently produced less damaged progenies than those from low E. saccharina infestation, indicating the occurrence of natural and recurrent selection. The results suggested presence of additive and non-additive genetic effects as well as ...

  10. Seleção de progênies de meio-irmãos de cenoura baseada em características de sementes Selection of carrot progenies based on seed characteristics

    Directory of Open Access Journals (Sweden)

    Jairo V. Vieira

    2005-03-01

    Full Text Available A menor germinação e emergência de plântulas em campo de cenoura obtida nas sementes de cultivares nacionais em relação às importadas, aumenta o custo de produção além de impossibilitar a otimização do estande final através do emprego de semeadeiras de precisão. Setenta e quatro progênies de meio-irmãos oriundas de uma população de cenoura tipo Brasília foram avaliadas quanto a germinação, vigor e peso de sementes com o objetivo de verificar a variabilidade e as relações genéticas entre os referidos caracteres. Evidenciou-se a existência de diferenças significativas entre as progênies de meio-irmãos em relação a todos os caracteres avaliados. Os altos valores de herdabilidade e variabilidade genética entre progênies para os caracteres avaliados sugerem que métodos de melhoramento simples combinados com métodos de seleção tendo por base a teoria de índice podem ser aplicados visando a melhoria da qualidade fisiológica das sementes de cenoura.Low seed germination and stand establishment of carrot seeds from Brazilian cultivars generally increase the production cost and impairs the use of precision seeders. Germination, vigor and seed weight of 74 progenies from a population of carrot cv. Brasilia were evaluated to verify the variability and the genetic relations among these three characteristics. Significant differences were observed among the progenies in relation to all characteristics. High values of heritability and genetic variability among progenies suggests that simple breeding methods combined with index selection methods may be applied during breeding programs in order to increase the physiological seed quality of carrot.

  11. A Radon Progeny Deposition Model

    Science.gov (United States)

    Guiseppe, V. E.; Elliott, S. R.; Hime, A.; Rielage, K.; Westerdale, S.

    2011-04-01

    The next generation low-background detectors operating underground aim for unprecedented low levels of radioactive backgrounds. Although the radioactive decays of airborne radon (particularly 222Rn) and its subsequent progeny present in an experiment are potential backgrounds, also problematic is the deposition of radon progeny on detector materials. Exposure to radon at any stage of assembly of an experiment can result in surface contamination by progeny supported by the long half life (22 y) of 210Pb on sensitive locations of a detector. An understanding of the potential surface contamination from deposition will enable requirements of radon-reduced air and clean room environments for the assembly of low background experiments. It is known that there are a number of environmental factors that govern the deposition of progeny onto surfaces. However, existing models have not explored the impact of some environmental factors important for low background experiments. A test stand has been constructed to deposit radon progeny on various surfaces under a controlled environment in order to develop a deposition model. Results from this test stand and the resulting deposition model are presented.

  12. Phenotypic recurrent selection to improve protein quality in non-opaque maize populations

    Directory of Open Access Journals (Sweden)

    Garcia Antonio Augusto Franco

    2002-01-01

    Full Text Available Maize (Zea mays L. protein is considered to be of low quality due to low levels of the essential lysine and tryptophan amino acids. An alternative to solve this problem is to use the opaque-2 gene, which improves the level of these amino acids, but has negative pleiotropic effects on agronomic characters. A phenotypic recurrent selection scheme was carried out in two non-opaque maize populations to verify the possibility of improving their protein quality without using this gene. Four cycles were completed and a 20% selection intensity for tryptophan content in the kernels was used in two populations, IG-1 and IG-2. The original and the four-cycle populations were evaluated in three locations for agronomic traits. For protein and tryptophan content, a separated trial was carried out because plants of the plots were hand-pollinated. No increase in tryptophan content was observed in the IG-2 population, whereas IG-1 presented a small increase (0.70% per cycle. The ratio tryptophan/protein increased 1.26% per cycle in IG-1 and the protein content did not increase in both populations. The ESALQ-VD2-opaque check was superior in relation to both populations for protein quality, as expected, even after completion of four selection cycles. The kernel yield (2.5% per cycle prolificacy, plant and ear heights, decreased with selection cycles, as a correlated response to selection. Phenotypic recurrent selection in non-opaque maize was not able to increase, at reasonable rates, the protein quality of maize kernels.

  13. Early growth performance of full-sib Acacia auriculiformis x Acacia mangium F1 hybrid progenies at three different sites

    Science.gov (United States)

    Shah Aimin, Atirah Abdullah; Abdullah, Mohd Zaki; Muhammad, Norwati; Ratnam, Wickneswari

    2014-09-01

    Field trials of 14 full sib Acacia auriculiformis x Acacia mangium F1 hybrid progenies were evaluated for growth performance at three sites (Bintulu, Mentakab and Segamat). Results indicated that there were significant differences (p> 0.05) for diameter breast height (Dbh) and total height (Ht) among the progenies and different sites. Superior progenies have been identified for future tree selection and improvement.

  14. Evaluation of selected recurrence measures in discriminating pre-ictal and inter-ictal periods from epileptic EEG data

    Energy Technology Data Exchange (ETDEWEB)

    Ngamga, Eulalie Joelle [Potsdam Institute for Climate Impact Research, Telegraphenberg A 31, 14473 Potsdam (Germany); Bialonski, Stephan [Max-Planck-Institute for the Physics of Complex Systems, Nöthnitzer Straße 38, 01187 Dresden (Germany); Marwan, Norbert, E-mail: marwan@pik-potsdam.de [Potsdam Institute for Climate Impact Research, Telegraphenberg A 31, 14473 Potsdam (Germany); Kurths, Jürgen [Potsdam Institute for Climate Impact Research, Telegraphenberg A 31, 14473 Potsdam (Germany); Department of Physics, Humboldt University Berlin, 12489 Berlin (Germany); Institute for Complex Systems and Mathematical Biology, University of Aberdeen, Aberdeen AB24 3UE (United Kingdom); Geier, Christian [Department of Epileptology, University of Bonn, Sigmund-Freud-Straße 25, 53105 Bonn (Germany); Helmholtz Institute for Radiation and Nuclear Physics, University of Bonn, Nussallee 14–16, 53115 Bonn (Germany); Lehnertz, Klaus [Department of Epileptology, University of Bonn, Sigmund-Freud-Straße 25, 53105 Bonn (Germany); Helmholtz Institute for Radiation and Nuclear Physics, University of Bonn, Nussallee 14–16, 53115 Bonn (Germany); Interdisciplinary Center for Complex Systems, University of Bonn, Brühler Straße 7, 53175 Bonn (Germany)

    2016-04-01

    We investigate the suitability of selected measures of complexity based on recurrence quantification analysis and recurrence networks for an identification of pre-seizure states in multi-day, multi-channel, invasive electroencephalographic recordings from five epilepsy patients. We employ several statistical techniques to avoid spurious findings due to various influencing factors and due to multiple comparisons and observe precursory structures in three patients. Our findings indicate a high congruence among measures in identifying seizure precursors and emphasize the current notion of seizure generation in large-scale epileptic networks. A final judgment of the suitability for field studies, however, requires evaluation on a larger database. - Highlights: • Recurrence-based analysis of brain dynamics in human epilepsy. • Comparison of recurrence quantification and recurrence network measures. • Statistically significant precursory structures in three out of five patients. • High congruence among measures in characterizing brain dynamics.

  15. Seleção recorrente intrapopulacional no maracujazeiro amarelo: alternativa de capitalização de ganhos genéticos Intrapopulation recurrent selection in yellow passion fruit: alternative to accumulate genetic gains

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    Marcelo Geraldo de Morais Silva

    2009-02-01

    Full Text Available Objetivou-se, neste trabalho, estimar parâmetros genéticos e correlações associadas a características agronômicas do maracujazeiro amarelo (Passiflora edulis Sims, predizer o progresso genético esperado com a seleção direta e baseado em índice de seleção, bem como sugerir uma estratégia de capitalização de ganhos genéticos nos ciclos de seleção recorrente. Foram avaliadas 26 progênies de meio-irmãos provenientes da recombinação de genótipos selecionados do primeiro ciclo de seleção via Delineamento I, as quais representam a população melhorada, UENF/MA1. O delineamento estatístico utilizado foi blocos casualizados com duas repetições e parcelas constituídas de cinco plantas. Foram avaliadas cinco características relacionadas ao fruto, no período de fevereiro a julho de 2007, utilizando dados de média de parcelas. As estimativas dos parâmetros genéticos e das correlações, bem como a seleção baseada no índice de seleção possibilitaram a identificação de progênies com desempenhos agronômicos superiores para vários caracteres, simultaneamente. Além disso, a presente estratégia pode constituir alternativa de capitalização de ganhos genéticos na seleção recorrente, aumentando a eficiência do processo seletivo e potencializando a sua utilização em programas de melhoramento de fruteiras, uma vez que permite ganho genético em duas (geração e teste de progênies das três etapas de seleção recorrente.The aim of this research was to estimate the genetic parameters and correlations associated to the agronomic traits of yellow passion fruit (Passiflora edulis Sims, to predict the expected genetic progress with the direct selection and based on selection index, as well as to suggest a strategy to accumulate genetic gains in the recurrent selection cycles. Twenty six half-sib progenies originated from the recombination of selected genotypes from the first cycle of selection via design I were

  16. The change of genetic and phenotypic variability of yield components after recurrent selection of maize

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    Deletić Nebojša

    2009-01-01

    Full Text Available This paper deals with 31 SSD lines from ZP-Syn-1 C0 and 37 from ZP-Syn-1 C3 maize populations. After line selection and seed multiplication in the first year of the study, the trials were set during two years in Kruševac and Zemun Polje, in RCB design with three replications. Additive and phenotypic variances of yield components were calculated, as well as the estimation of genetic variability narrowing by multivariate cluster analysis. The differences in additive and phenotypic variances between the cycles were significant for ear length only and highly significant for grain row number per ear and for percent of root and stalk lodged plants. It means, a significant narrowing of additive and phenotypic variance occurred only for those three traits, and the other traits did not change their variability by selection in a significant manner. However, according to cluster analysis, distances among genotypes and groups in the zero selection cycle were approximately double than in the third one, but group definition was better in the third selection cycle. It can suggest indirectly to a total variability narrowing after three cycles of recurrent selection.

  17. Phenotypic recurrent selection on herb growth yield of citronella grass (Cymbopogon nardus grown in Egypt

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    MOHAMED M. IBRAHIM

    2013-11-01

    Full Text Available Ibrahim MM,Khalid KA. 2013. Phenotypic recurrent selection on herb growth yield of citronella grass (Cymbopogon nardus grown in Egypt. Nusantara Bioscience 5: 68-72. This investigation was conducted in four generations: base population (G0, G1, G2 and G3 (clone selection generation to evaluate the genetic variability of citronella clones. Thirteen clones were selected from base population to study the herb growth yield characters and oil production as well as genetic parameters, correlation and regression. Results were recorded for herb growth characters (i.e. plant high (PH, no. of tillers (NOT, dry yield (DY, viability percentage (VP and oil production. Significant variation was observed among citronella clones in base population for most studied traits. Wide range of mean values was observed among the characters for generations and cuts in most of traits. High heritability values (0.95, 0.93, 0.89 and 0.72 were estimated in NOT, LG, HY and VP., respectively. Clone code no. 39/3, 17/4 and 8/1 gave highest values of dry weight, oil yield and viability percentage. Selected clones showed significant positive regression and correlation between dry weight and each of number of tillers and linear growth. On contrary, viability percentage had significant negative correlation and regression with other characters. These results raveled high yielding selected citronella clones will be utilized in medicinal plant breeding program.

  18. Genetic parameters and estimated genetic gains in young rubber tree progenies

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    Cecília Khusala Verardi

    2013-04-01

    Full Text Available The objective of this work was to assess the genetic parameters and to estimate genetic gains in young rubber tree progenies. The experiments were carried out during three years, in a randomized block design, with six replicates and ten plants per plot, in three representative Hevea crop regions of the state of São Paulo, Brazil. Twenty-two progenies were evaluated, from three to five years old, for rubber yield and annual girth growth. Genetic gain was estimated with the multi-effect index (MEI. Selection by progenies means provided greater estimated genetic gain than selection based on individuals, since heritability values of progeny means were greater than the ones of individual heritability, for both evaluated variables, in all the assessment years. The selection of the three best progenies for rubber yield provided a selection gain of 1.28 g per plant. The genetic gains estimated with MEI using data from early assessments (from 3 to 5-year-old were generally high for annual girth growth and rubber yield. The high genetic gains for annual girth growth in the first year of assessment indicate that progenies can be selected at the beginning of the breeding program. Population effective size was consistent with the three progenies selected, showing that they were not related and that the population genetic variability is ensured. Early selection with the genetic gains estimated by MEI can be made on rubber tree progenies.

  19. Low-dose aspirin, non-steroidal anti-inflammatory drugs, selective COX-2 inhibitors and breast cancer recurrence

    DEFF Research Database (Denmark)

    Cronin-Fenton, Deirdre P; Heide-Jørgensen, Uffe; Ahern, Thomas P

    2016-01-01

    BACKGROUND: Aspirin, nonsteroidal anti-inflammatory drugs (NSAIDs), and selective COX-2 inhibitors may improve outcomes in breast cancer patients. We investigated the association of aspirin, NSAIDs, and use of selective COX-2 inhibitors with breast cancer recurrence. METHODS: We identified incide...

  20. Selective Silencing of Hippocampal Parvalbumin Interneurons Induces Development of Recurrent Spontaneous Limbic Seizures in Mice.

    Science.gov (United States)

    Drexel, Meinrad; Romanov, Roman A; Wood, James; Weger, Stefan; Heilbronn, Regine; Wulff, Peer; Tasan, Ramon O; Harkany, Tibor; Sperk, Günther

    2017-08-23

    Temporal lobe epilepsy (TLE) is the most frequent form of focal epilepsies and is generally associated with malfunctioning of the hippocampal formation. Recently, a preferential loss of parvalbumin (PV) neurons has been observed in the subiculum of TLE patients and in animal models of TLE. To demonstrate a possible causative role of defunct PV neurons in the generation of TLE, we permanently inhibited GABA release selectively from PV neurons of the ventral subiculum by injecting a viral vector expressing tetanus toxin light chain in male mice. Subsequently, mice were subjected to telemetric EEG recording and video monitoring. Eighty-eight percent of the mice presented clusters of spike-wave discharges (C-SWDs; 40.0 ± 9.07/month), and 64% showed spontaneous recurrent seizures (SRSs; 5.3 ± 0.83/month). Mice injected with a control vector presented with neither C-SWDs nor SRSs. No neurodegeneration was observed due to vector injection or SRS. Interestingly, mice that presented with only C-SWDs but no SRSs, developed SRSs upon injection of a subconvulsive dose of pentylenetetrazole after 6 weeks. The initial frequency of SRSs declined by ∼30% after 5 weeks. In contrast to permanent silencing of PV neurons, transient inhibition of GABA release from PV neurons through the designer receptor hM4Di selectively expressed in PV-containing neurons transiently reduced the seizure threshold of the mice but induced neither acute nor recurrent seizures. Our data demonstrate a critical role for perisomatic inhibition mediated by PV-containing interneurons, suggesting that their sustained silencing could be causally involved in the development of TLE.SIGNIFICANCE STATEMENT Development of temporal lobe epilepsy (TLE) generally takes years after an initial insult during which maladaptation of hippocampal circuitries takes place. In human TLE and in animal models of TLE, parvalbumin neurons are selectively lost in the subiculum, the major output area of the hippocampus. The present

  1. Fast growth increases the selective advantage of a mutation arising recurrently during evolution under metal limitation.

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    Hsin-Hung Chou

    2009-09-01

    Full Text Available Understanding the evolution of biological systems requires untangling the molecular mechanisms that connect genetic and environmental variations to their physiological consequences. Metal limitation across many environments, ranging from pathogens in the human body to phytoplankton in the oceans, imposes strong selection for improved metal acquisition systems. In this study, we uncovered the genetic and physiological basis of adaptation to metal limitation using experimental populations of Methylobacterium extorquens AM1 evolved in metal-deficient growth media. We identified a transposition mutation arising recurrently in 30 of 32 independent populations that utilized methanol as a carbon source, but not in any of the 8 that utilized only succinate. These parallel insertion events increased expression of a novel transporter system that enhanced cobalt uptake. Such ability ensured the production of vitamin B(12, a cobalt-containing cofactor, to sustain two vitamin B(12-dependent enzymatic reactions essential to methanol, but not succinate, metabolism. Interestingly, this mutation provided higher selective advantages under genetic backgrounds or incubation temperatures that permit faster growth, indicating growth-rate-dependent epistatic and genotype-by-environment interactions. Our results link beneficial mutations emerging in a metal-limiting environment to their physiological basis in carbon metabolism, suggest that certain molecular features may promote the emergence of parallel mutations, and indicate that the selective advantages of some mutations depend generically upon changes in growth rate that can stem from either genetic or environmental influences.

  2. Estimate of genetic gain in popcorn after cycles of phenotypic recurrent selection.

    Science.gov (United States)

    Ematné, H J; Nunes, J A R; Dias, K O G; Prado, P E R; Souza, J C

    2016-05-20

    Popcorn is widely consumed in Brazil, yet there are few breeding programs for this crop. Recurrent selection (RS) is a viable breeding alternative for popcorn; however, the gains achieved must be frequently checked. The aim of this study was to assess the effect of selection for grain type (round and pointed) after four cycles of phenotypic RS on the main agronomic traits of popcorn, to estimate the genetic gain achieved for the trait of expansion volume (EV), and to obtain estimates of phenotypic correlations for the main traits of the crop in the UFLA E and UFLA R populations. The zero, one, two, and three cycles of the UFLA E and UFLA R populations, the fourth cycle, and the controls IAC-112 and IAC-125 were used. The experiments were conducted at the experimental farm of Universidade Federal de Lavras (UFLA; Environment 1) and at the experimental area of the Genetics and Plant Breeding Sector of the Department of Biology at UFLA (Environment 2) in the 2010/11 crop season. Nine agronomic traits were evaluated, including EV and grain yield (GY). The UFLA R and UFLA E populations showed similar behavior for all evaluated traits. The type of grain did not affect the genetic gain for EV, which was 5 and 3.7% in each cycle carried out in the UFLA E and UFLA R population, respectively. Phenotypic selection carried out during recombination for EV is an effective method for increasing expression of the trait. EV and GY did not show a linear association.

  3. Persistency of Prediction Accuracy and Genetic Gain in Synthetic Populations Under Recurrent Genomic Selection

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    Dominik Müller

    2017-03-01

    Full Text Available Recurrent selection (RS has been used in plant breeding to successively improve synthetic and other multiparental populations. Synthetics are generated from a limited number of parents ( Np , but little is known about how Np affects genomic selection (GS in RS, especially the persistency of prediction accuracy (rg , g ^ and genetic gain. Synthetics were simulated by intermating Np= 2–32 parent lines from an ancestral population with short- or long-range linkage disequilibrium (LDA and subjected to multiple cycles of GS. We determined rg , g ^ and genetic gain across 30 cycles for different training set (TS sizes, marker densities, and generations of recombination before model training. Contributions to rg , g ^ and genetic gain from pedigree relationships, as well as from cosegregation and LDA between QTL and markers, were analyzed via four scenarios differing in (i the relatedness between TS and selection candidates and (ii whether selection was based on markers or pedigree records. Persistency of rg , g ^ was high for small Np , where predominantly cosegregation contributed to rg , g ^ , but also for large Np , where LDA replaced cosegregation as the dominant information source. Together with increasing genetic variance, this compensation resulted in relatively constant long- and short-term genetic gain for increasing Np > 4, given long-range LDA in the ancestral population. Although our scenarios suggest that information from pedigree relationships contributed to rg , g ^ for only very few generations in GS, we expect a longer contribution than in pedigree BLUP, because capturing Mendelian sampling by markers reduces selective pressure on pedigree relationships. Larger TS size (NTS and higher marker density improved persistency of rg , g ^ and hence genetic gain, but additional recombinations could not increase genetic gain.

  4. Allohexaploid pearl millet x elephantgrass population potential for a recurrent selection program Potencial da população alohexaplóide de milheto x capim-elefante para um programa de seleção recorrente

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    Matheus Costa dos Reis

    2008-02-01

    Full Text Available The objective of this work was to evaluate the potential of allohexaploid pearl millet x elephantgrass (HGL population for a recurrent selection program through open-pollinated progenies. Seventy-eight progenies, one representative sample of the population, and two commercial cultivars, Pioneiro and Paraíso, were evaluated in a 9x9 triple lattice design, in two sites. Plant height and dry matter yield were evaluated in three and four cuts, respectively. For plant height, the 17 best progenies were similar to both commercial controls, while for dry matter yield they were higher than 'Paraíso' and lower than 'Pioneiro'. The correlation between progenies and cuts indicated that the fourth cut represents the mean of all cuts, and the possibility of using early selection. Heritability estimates considering cuts and sites were 56.9% for plant height and 58.8% for dry matter yield, and the expected response to selection was 23.4% for dry matter yield and 18.1% for plant height. These results demonstrate the promising HGL population potential for a recurrent selection program.O objetivo deste trabalho foi avaliar o potencial para programa de seleção recorrente, em uma população alohexaplóide de milheto x capim-elefante (HGL, por meio de progênies de polinização aberta. Setenta e oito progênies, uma testemunha representativa da população e duas cultivares comerciais, a Pioneiro e a Paraíso, foram avaliadas no delineamento látice triplo 9x9, em dois locais. Os caracteres altura de plantas e produtividade de matéria seca foram avaliados por três e quatro cortes, respectivamente. Quanto à altura de plantas, a média das 17 melhores progênies foi similar às das testemunhas comerciais e, quanto à produtividade de matéria seca, foi maior do que a da 'Paraíso' e menor do que a da 'Pioneiro'. A correlação entre progênies e colheitas mostrou que a quarta colheita representa a média de todas as colheitas e a possibilidade de sele

  5. Comparison of two methods of reciprocal recurrent selection in maize (Zea mays L.).

    Science.gov (United States)

    Ordas, B; Butron, A; Alvarez, A; Revilla, P; Malvar, R A

    2012-05-01

    Reciprocal recurrent selection (RRS) was proposed for simultaneously improving two populations and their cross. A modification of the classical full-sib RRS (FS-RRS) was proposed in which the performance of full-sibs and S(2) families is combined in a selection index (FS-S(2)-RRS). The Mediterranean corn borer (MCB) is the main corn borer species in the Mediterranean and adjacent areas and produces important yield losses. We started two RRS programs (FS-RRS and FS-S(2)-RRS) from the same maize population in which the selection criterion was grain yield under artificial infestation with MCB eggs. Original populations, two cycles of selection derived from them by both RRS methods, and population crosses were evaluated under MCB attack and under insecticide treatment in three different environments. The objective was to compare the efficiency of the FS-RRS and the FS-S(2)-RRS methods for improving grain yield. We found that the FS-S(2)-RRS method was successful for improving the yield of the population cross under optimum conditions (the regression coefficient over cycles was b = 0.87** Mg ha(-1) cycle(-1)) without losing yield under high pressure of MCB attack (b = 0.07). On the contrary, FS-RRS failed to improve the yield of the population cross under optimum conditions (b = 0.65) and tended to decrease the yield under high levels of MCB attack (b = -0.26). We conclude that for developing high yielding and stable varieties, FS-S(2)-RRS is more efficient than the classical FS-RRS method.

  6. Effect of recurrent selection on the variability of the UENF-14 popcorn population

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    Rodrigo Moreira Ribeiro

    2016-07-01

    Full Text Available This study aimed to evaluate the effect of recurrent selection on the genetic variability of UENF-14 population after six selections. Two hundred and ten half-sib families were evaluated in two environments in the state of Rio de Janeiro, using incomplete randomized blocks design with treatments arranged in replication within “Sets”. There was significant effect for Families within the “Set” (F/S, proving that there is enough genetic variability to be exploited in the popcorn breeding program of UENF. The significance for the source of variation Environment (E shows that the environments were distinct enough to promote differences between the evaluated characteristics. It was found that for both characteristics of greatest interest, GY and PE, the magnitude of the additive variance remains with close values in advanced cycles of UENF-14 population, indicating that variability remains, with no evidence of decreases in advanced cycles. This is concluded by the longevity of UENF breeding program.

  7. Recurrent selection of cocoa populations in Cote d'Ivoire: comparative genetic diversity between the first and second cycles

    Science.gov (United States)

    In Cote d'Ivore, the cocoa breeding programme has been based on the creation of hybrids between different genetic groups. From 1990 onward, a reciprocal recurrent selection programme has been set up with the purpose of improving simultaneously the characteristics of the two main genetic groups: Uppe...

  8. Delay selection by spike-timing-dependent plasticity in recurrent networks of spiking neurons receiving oscillatory inputs.

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    Robert R Kerr

    Full Text Available Learning rules, such as spike-timing-dependent plasticity (STDP, change the structure of networks of neurons based on the firing activity. A network level understanding of these mechanisms can help infer how the brain learns patterns and processes information. Previous studies have shown that STDP selectively potentiates feed-forward connections that have specific axonal delays, and that this underlies behavioral functions such as sound localization in the auditory brainstem of the barn owl. In this study, we investigate how STDP leads to the selective potentiation of recurrent connections with different axonal and dendritic delays during oscillatory activity. We develop analytical models of learning with additive STDP in recurrent networks driven by oscillatory inputs, and support the results using simulations with leaky integrate-and-fire neurons. Our results show selective potentiation of connections with specific axonal delays, which depended on the input frequency. In addition, we demonstrate how this can lead to a network becoming selective in the amplitude of its oscillatory response to this frequency. We extend this model of axonal delay selection within a single recurrent network in two ways. First, we show the selective potentiation of connections with a range of both axonal and dendritic delays. Second, we show axonal delay selection between multiple groups receiving out-of-phase, oscillatory inputs. We discuss the application of these models to the formation and activation of neuronal ensembles or cell assemblies in the cortex, and also to missing fundamental pitch perception in the auditory brainstem.

  9. Recurrent Selection and Participatory Plant Breeding for Improvement of Two Organic Open-Pollinated Sweet Corn (Zea mays L. Populations

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    Adrienne C. Shelton

    2015-04-01

    Full Text Available Organic growers face unique challenges when raising sweet corn, and benefit from varieties that maintain high eating quality, germinate consistently, deter insect pests, and resist diseases. Genotype by environment rank changes can occur in the performance of cultivars grown on conventional and organic farms, yet few varieties have been bred specifically for organic systems. The objective of this experiment was to evaluate the changes made to open-pollinated sweet corn populations using recurrent selection and a participatory plant breeding (PPB methodology. From 2008 to 2011, four cycles of two open-pollinated (OP sweet corn populations were selected on a certified organic farm in Minnesota using a modified ear-to-row recurrent selection scheme. Selections were made in collaboration with an organic farmer, with selection criteria based on traits identified by the farmer. In 2012 and 2013, the population cycles were evaluated in a randomized complete block design in two certified organic locations in Wisconsin, with multiple replications in each environment. Significant linear trends were found among cycles of selection for quantitative and qualitative traits, suggesting the changes were due to recurrent selection and PPB methodology for these populations. However, further improvement is necessary to satisfy the requirements for a useful cultivar for organic growers.

  10. Selective genomic copy number imbalances and probability of recurrence in early-stage breast cancer.

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    Patricia A Thompson

    Full Text Available A number of studies of copy number imbalances (CNIs in breast tumors support associations between individual CNIs and patient outcomes. However, no pattern or signature of CNIs has emerged for clinical use. We determined copy number (CN gains and losses using high-density molecular inversion probe (MIP arrays for 971 stage I/II breast tumors and applied a boosting strategy to fit hazards models for CN and recurrence, treating chromosomal segments in a dose-specific fashion (-1 [loss], 0 [no change] and +1 [gain]. The concordance index (C-Index was used to compare prognostic accuracy between a training (n = 728 and test (n = 243 set and across models. Twelve novel prognostic CNIs were identified: losses at 1p12, 12q13.13, 13q12.3, 22q11, and Xp21, and gains at 2p11.1, 3q13.12, 10p11.21, 10q23.1, 11p15, 14q13.2-q13.3, and 17q21.33. In addition, seven CNIs previously implicated as prognostic markers were selected: losses at 8p22 and 16p11.2 and gains at 10p13, 11q13.5, 12p13, 20q13, and Xq28. For all breast cancers combined, the final full model including 19 CNIs, clinical covariates, and tumor marker-approximated subtypes (estrogen receptor [ER], progesterone receptor, ERBB2 amplification, and Ki67 significantly outperformed a model containing only clinical covariates and tumor subtypes (C-Index(full model, train[test]  =  0.72[0.71] ± 0.02 vs. C-Index(clinical + subtype model, train[test]  =  0.62[0.62] ± 0.02; p<10(-6. In addition, the full model containing 19 CNIs significantly improved prognostication separately for ER-, HER2+, luminal B, and triple negative tumors over clinical variables alone. In summary, we show that a set of 19 CNIs discriminates risk of recurrence among early-stage breast tumors, independent of ER status. Further, our data suggest the presence of specific CNIs that promote and, in some cases, limit tumor spread.

  11. Seleção para a resistência à ferrugem em progênies das cultivares de café IPR 99 e IPR 107 Selection for resistance to rust in progenies of coffee cultivars IPR 99 and IPR 107

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    Gustavo Hiroshi Sera

    2010-01-01

    Full Text Available As cultivares de café IPR 99 ("Sarchimor" e IPR 107 ('IAPAR 59' x 'Mundo Novo IAC 376-4' eram no passado resistentes à ferrugem (Hemileia vastatrix. Os objetivos deste estudo foram: a Identificar progênies dessas duas cultivares com resistência à ferrugem; b Identificar progênies com resistência incompleta; c Verificar a eficiência dos cruzamentos testes na seleção dessas cultivares. A avaliação da resistência em campo foi realizada em cafeeiros adultos expostos à população local de raças presentes no IAPAR. Foram avaliados 23 cruzamentos testes com progênies F3 de 'IPR 107' e 5 com progênies F4 de 'IPR 99'. Além disso, foram avaliadas 11 progênies F4 de 'IPR 107' e 5 progênies F5 de 'IPR 99', provenientes de autofecundação. Várias progênies das cultivares IPR 99 e 107 apresentaram alta freqüência de plantas com resistência completa e são portadoras de mais genes de resistência não quebrados pelas raças de ferrugem. Progênies das cultivares IPR 99 e IPR 107 com a resistência quebrada apresentaram resistência incompleta à população local de raças. Cruzamentos testes foram eficientes na seleção de progênies de café com mais genes de resistência não quebrados.All plants of the coffee cultivars IPR 99 ("Sarchimor" and IPR 107 ('IAPAR 59' x 'Mundo Novo IAC 376-4' were resistant to rust (Hemileia vastatrix in the past. Currently, susceptible plants were observed in the two cultivars due to the breakdown of resistance by new rust races. The aims of this study were: a to identify coffee (Coffea arabica L. progenies of the two cultivars with resistance to rust; b to identify progenies with incomplete resistance; c to investigate the efficiency of test-crosses for selection from these cultivars. Evaluation for field resistance was carried out in adult plants subjected to natural infection by the local leaf rust population at IAPAR. Twenty-three test-crosses with F3 progenies of 'IPR 107' and five with F4

  12. IDENTIFIKASI GEN AROMA PADA PROGENI-PROGENI BACKCROSS ANTARA VARIETAS CIHERANG DENGAN PANDAN WANGI

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    Djarot Sasongko Hami Seno

    2011-08-01

    Full Text Available Marker-assisted PCR has been considered as the most potential method for fragrant selection. RM223 is the only suitable marker to identify mutated badh2 gene of Pandan Wangi. This research applies RM223-assisted PCR in the introgression of fragrant gene (mutated badh2 of Pandan Wangi variety, to engineer non-transgenic fragrant variety with good agronomic traits as those of Ciherang. Gene introduction was carried out through site-directed crossing; Pandan Wangi was crossed and backcrossed to Ciherang until heterozygot BC5F1, followed by selfing to obtain homozygot BC5F2. RM223-assisted selection was conducted in each cross and backcross generation. RM223 was able to identify native, mutated and heterozygot badh2 of Ciherang, Pandan Wangi, and their cross/backcross progenies, respectively. Therefore, the introgression of mutated badh2 within progenies were observed, as well as the statues of badh2 gene (native/mutated and alleles (homozygot/heterozygot. Further backcross and selfing to obtain BC5F2 is in progress

  13. Relation of Zn, Fe, Cu and Mn between progenies of mate-tree and soil

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    Josiane Cava Guimarães

    2014-06-01

    Full Text Available The mate tea tree (Ilex Paraguariensis St. Hil. has considerable growth in acid a low fertility soils. The knowledge of soil and plant relation will contribute to genetic improvement programs, as highly capable progenies in nutrient acquisition may be selected. The objective of this study was to evaluate the interference of provenance and, or progenies, in relations established among the extractable contents of Zn, Fe, Cu and Mn, via Mehlich-1 (1:10; and EDTA (1%, with the corresponding contents in the mate tea tree leaves. In the experiment two provenances, Ivaí-PR and Barão de Cotegipe-RS, with five progenies each considered as treatments. The samples were distributed in four randomized blocks, totalizing 120 plant leaf samples, related to 120 soil samples of a Red Distrophic Latosol. The results were analyzed and fitted in regression equations. In Ivaí provenance Zn and Mn from the soil correlated with their contents in the leaves for progeny 04, for both extracts. For provenances 08 and 10, soil Mn via EDTA correlated with leaf Mn contents, while via Mehlich-1 only for progeny 10. In the provenance of Barão de Cotegipe, the correlations between soil and leaves for Zn, Fe and Cu occurred for the EDTA extract in the progenies 61, 65 and 69 respectively. For Mn and Cu, via Melich-1 the correlations occurred for progenies 53 and 69 respectively, and still for Cu, via EDTA, for progeny 53.

  14. Variabilidade de progênies F2 de amendoim geradas por meio de seleção de genitores ISSR-divergentes Variability of F2 peanut progenies generated through the selection of ISSR-divergent genitors

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    Roseane Cavalcanti dos Santos

    2013-09-01

    % monomorphic and low contribution to studies of access discrimination using ISSR markers. Three distinct clusters were formed in the dendrogram, among which two genotypes with greater divergence, represented by cultivar BR 1 (short cycle and upright and line LViPE-06 (late cycle and runner, were selected for hybridization to generate genetic variability. The resulting F2 progenies showed broad genetic variability, with large contribution to selection procedures for the to assist selection procedures in peanut. Based on the results obtained, it was possible to confirm the contribution of molecular tools to assist selection procedures in peanut.

  15. Três ciclos de seleção entre e dentro de progênies de meios-irmãos na população de milho CMS-52 Three cycles selection among and within half sib progenies in the maize population CMS-52

    Directory of Open Access Journals (Sweden)

    HÉLIO WILSON LEMOS DE CARVALHO

    2000-08-01

    Full Text Available Três ciclos de seleção entre e dentro de progênies de meios-irmãos foram praticados na população de milho (Zea mays L. de alta qualidade protéica CMS-52, nos tabuleiros costeiros dos estados de Sergipe e Bahia, no período de 1995 a 1997, visando à obtenção de uma população melhor adaptada às condições edafoclimáticas da região. As progênies foram avaliadas em látice simples 14 x 14, com recombinação das progênies superiores, dentro do mesmo ano agrícola, de modo a se obter um ciclo por ano. Os valores dos parâmetros genéticos decresceram do ciclo original para o ciclo I, mantendo-se no ciclo II com magnitudes semelhantes ao ciclo I. As altas magnitudes desses parâmetros genéticos, as altas médias de produtividades das progênies, e o ganho médio esperado com a seleção entre e dentro de progênies, por ciclo de seleção (12,3%, mostram o grande potencial da população em responder à seleção, o que permitirá a obtenção de uma população mais produtiva e melhor adaptada às condições edafoclimáticas da região. A magnitude da interação progênies x locais evidenciou a importância de se avaliarem as progênies em mais de um local, para melhorar a eficiência do processo seletivo e obter estimativas mais consistentes dos componentes da variância.Three cycles of selection among and within halfsib progenies were carried out in the high quality protein maize (Zea mays L. population CMS-52, at the tabuleiros costeiros of Sergipe and Bahia, Brazil, during the period of 1995 to 1997. The work aimed at obtaining a better adapted population for the regional environment. The progenies were evaluated on a 14 x 14 látice design. Recombination of the superior progenies was processed within the same year in order to get one cycle per year. The values of the genetic parameters decreased from the original cycle to cycle I, keeping at cycle II similar magnitudes of cycle I. The high magnitudes of the genetic

  16. Selective attention and fear of cancer recurrence in breast cancer survivors

    NARCIS (Netherlands)

    Custers, J.A.E.; Becker, E.S.; Gielissen, M.F.M.; Laarhoven, H.W.M. van; Rinck, M.; Prins, J.B.

    2015-01-01

    BACKGROUND: Anxious people show an attentional bias towards threatening information. PURPOSE: It was investigated whether an attentional bias exists for cancer-related stimuli in breast cancer survivors and if different levels of fear of cancer recurrence would lead to different patterns of

  17. RELATIONSHIP BETWEEN YIELD AND FRUIT QUALITY OF PASSION FRUIT C03 PROGENIES UNDER DIFFERENT NUTRITIONAL LEVELS

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    CARLOS LACY SANTOS

    Full Text Available ABSTRACT The present study was conducted to evaluate different fertilization-management strategies in ten progenies of passion fruit from the third recurrent selection cycle and their effects on yield and fruit-quality traits. For this purpose, we adopted the strategy of correlations analysis, using the phenotypic and path correlations in different environmental conditions characterized by three levels of fertilization. The trial was set up as a randomized-block design in a split-plot arrangement with progenies representing the plots and three levels of potassium-nitrogen fertilization as the sub-plots, with three replicates. Path analysis showed that number of fruits was the variable of highest correlation with fruit diameter at fertilization I. Fruit weight and pulp weight were correlated with each other and with other traits like fruit length and fruit diameter at the three fertilization levels, except for number of fruits, which was correlated with nitrogen and potassium only at fertilization II. Path analysis also revealed that fruit diameter (3.125 showed the highest direct effect on yield at fertilization I. However, fruit weight and number of fruits showed, at fertilization II, the highest direct effects of 2.964 and 1.134 on yield, respectively, and number of fruits had a high phenotypic correlation and direct effect on yield at the three fertilization levels: 0.528 at fertilization I; 2.206 at fertilization II; and 0.928 at fertilization III. The results demonstrate the greater direct effect obtained with fertilization II, suggesting that the level adopted at fertilization II can provide satisfactory gains in yield and is thus recommended for the population in question.

  18. Prediction of direct and indirect genetic gains and genotypic correlations in rubber tree progenies

    Directory of Open Access Journals (Sweden)

    Cecília Khusala Verardi

    2011-09-01

    Full Text Available The objective of this work was to estimate the genetic parameters, genotypic and phenotypic correlations, and direct and indirect genetic gains among and within rubber tree (Hevea brasiliensis progenies. The experiment was set up at the Municipality of Jaú, SP, Brazil. A randomized complete block design was used, with 22 treatments (progenies, 6 replicates, and 10 plants per plot at a spacing of 3x3 m. Three‑year‑old progenies were assessed for girth, rubber yield, and bark thickness by direct and indirect gains and genotypic correlations. The number of latex vessel rings showed the best correlations, correlating positively and significantly with girth and bark thickness. Selection gains among progenies were greater than within progeny for all the variables analyzed. Total gains obtained were high, especially for girth increase and rubber yield, which were 93.38 and 105.95%, respectively. Young progeny selection can maximize the expected genetic gains, reducing the rubber tree selection cycle.

  19. Genetic gain prediction on UNB-2U popcorn population under recurrent selection by using different selection indexes / Predição de ganhos genéticos na população de milho pipoca UNB-2U sob seleção recorrente utilizando-se diferentes índices de seleção

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    Alexandre Pio Viana

    2009-12-01

    Full Text Available Aiming to estimate the gain prediction by using selection indexes on cycle C4 of UNB-2U, two hundred progenies of full-sib families were obtained on the third cycle under recurrent selection. The progenies were evaluated in a randomized complete block design with two replications within sets, in two distinct environments: Colégio Estadual Agrícola Antônio Sarlo, located in Campos dos Goytacazes, and Experimental Station of PESAGRO-Rio, located in Itaocara, Rio de Janeiro State. The utilized selection indexes were the Smith (1936 and Hazel (1943, Pesek and Baker (1969, Williams (1962, and Mulamba and Mock (1978. The selection index of Mulamba and Mock (1978 allowed the prediction of negative gains for number of disease ears, number of ears attacked by insects, number of broken plants, poorly hulled ears; and it provided superior gains for popcorn expansion and grain yield, with values of 10.55% and 8.50%, respectively, in the use of arbitrary weights attributed by tentative.Com o intento de estimar a predição de ganhos por índices de seleção no ciclo C4 de UNB-2U, duzentas famílias de irmãos-completos foram obtidas a partir da população de terceiro ciclo de seleção recorrente. As progênies foram avaliadas no delineamento de blocos casualizados com duas repetições dentro de ‘‘sets’’ em dois ambientes distintos: Colégio Estadual Agrícola Antônio Sarlo, em Campos dos Goytacazes, e Estação Experimental da PESAGRO-RIO, em Itaocara, Rio de Janeiro. Os índices de seleção empregados para predizer os ganhos foram os de Mulamba e Mock (1978, Pesek e Baker (1969, Smith (1936 e Hazel (1943 e Williams (1962. O índice de seleção Mulamba e Mock (1978 além de permitir a predição de ganhos negativos para número de espigas doentes e atacadas por pragas, número de plantas quebradas e acamadas, e espigas mal empalhadas; para capacidade de expansão e rendimento dos grãos, proporcionou ganhos superiores aos demais

  20. Predição de ganhos genéticos por índices de seleção na população de milho pipoca UNB-2U sob seleção recorrente Genetic gain prediction by selection index in a UNB-2U popcorn population under recurrent selection

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    Fabrício Santana Santos

    2007-01-01

    Full Text Available Com o intento de selecionar progênies superiores para a efetiva concentração de alelos favoráveis em uma população de terceiro ciclo de seleção recorrente intrapopulacional em famílias de meios-irmãos de milho-pipoca, avaliaram-se 192 progênies em delineamento em blocos casualizados com duas repetições em dois ambientes distintos: Colégio Estadual Agrícola Antônio Sarlo, em Campos dos Goytacazes, e Estação Experimental da PESAGRO-RIO, em Itaocara, Rio de Janeiro, em 2004/05. Definiu-se a intensidade de seleção de 15,6%, correspondendo ao número de trinta famílias de meios-irmãos para continuidade do programa. Foram empregados os índices de seleção de Smith e Hazel, Pesek e Baker, Williams, e Mulamba e Mock. O índice de Mulamba e Mock proporcionou as magnitudes mais elevadas de ganhos preditos para a maioria das características, incluindo-se capacidade de expansão e produção, com valores respectivos de 7,16% e 10,00%, na utilização de pesos arbitrários atribuídos por tentativas.Aiming to select superior progenies for concentration of favorable alleles in a popcorn population on the third cycle of intrapopulation half-sib recurrent selection, one hundred and ninety-two progenies were evaluated in a completed randomized design with two replications, in two distinct environments: Colégio Estadual Agrícola Antônio Sarlo, located in Campos dos Goytacazes, and Experimental Station of PESAGRO-RIO, located in Itaocara, Rio de Janeiro State, in 2004/05. The selection intensity was 15.6% that keep in touch thirty half-sib families for the continuity of the breeding program. The utilized selection indexes were the SMITH and HAZEL, PESEK and BAKER, WILLIAMS, and MULAMBA and MOCK. The MULAMBA and MOCK index propitiated more elevated magnitudes of the predicted gains for most of the traits, including popping expansion and grain yield, with values of 7.16% and 10.00%, respectively, utilizing arbitrary weight attributed by

  1. Seleção recorrente recíproca na obtenção de híbridos interpopulacionais de milho-pipoca Reciprocal recurrent selection to obtain interpopulation hybrids of popcorn

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    Vinícius Ribeiro Faria

    2008-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência da seleção recorrente recíproca em produzir híbridos de milho-pipoca (Zea mays de progênies endógamas superiores, com famílias de irmãos-completos. O programa de seleção recorrente recíproca envolveu as populações de milho-pipoca 'Viçosa' e 'Beija-Flor'. Os testes dos híbridos S0xS0 e S1xS1 foram conduzidos em delineamento látice, nos anos agrícolas de 2002/2003 e 2004/2005. Os dois ensaios incluíram testemunhas comerciais comuns, que permitiram a comparação do desempenho dos híbridos. Analisaram-se a produtividade e a capacidade de expansão. Foram preditos ganhos direto e indireto com a seleção em capacidadede expansão, avaliada em pipoqueira de ar quente e na pipocadora "Metric Weight Volume Tester". Os ganhos observados foram calculados para avaliar a eficiência da seleção recorrente recíproca. As análises de covariância mostraram variabilidade genotípica nas populações. Em relação às testemunhas comerciais, os híbridos S0xS0 foram inferiores em qualidade e equivalentes em produtividade, e os híbridos S1xS1 foram equivalentes nas duas características. O ganho observado na capacidade de expansão foi substancial, cerca de 4 mL g-1, superior ao ganho predito (2,8 mL g-1. A redução observada na produtividade não foi relevante. Verificou-se redução na variabilidade genotípica quanto à capacidade de expansão. O método de seleção recorrente recíproca foi eficiente em produzir híbridos S1xS1 superiores aos S0xS0.The objective of this work was to evaluate the efficiency of reciprocal recurrent selection in producing hybrids of popcorn (Zea mays from superior endogamous progenies, with full-sibling families. The reciprocal recurrent selection program involved the 'Viçosa' and 'Beija-Flor' popcorn populations. Tests of the hybrids S0xS0 and S1xS1 were carried out in lattice design, in the agricultural years 2002/2003 and 2004/2005. The two assays

  2. High Correlated Paternity Leads to Negative Effects on Progeny Performance in Two Mediterranean Shrub Species.

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    Sofia Nora

    Full Text Available Anthropogenic habitat deterioration can promote changes in plant mating systems that subsequently may affect progeny performance, thereby conditioning plant recruitment for the next generation. However, very few studies yet tested mating system parameters other than outcrossing rates; and the direct effects of the genetic diversity of the pollen received by maternal plants (i.e. correlated paternity has often been overlooked. In this study, we investigated the relation between correlated paternity and progeny performance in two common Mediterranean shrubs, Myrtus communis and Pistacia lentiscus. To do so, we collected open-pollinated progeny from selected maternal plants, calculated mating system parameters using microsatellite genotyping and conducted sowing experiments under greenhouse and field conditions. Our results showed that some progeny fitness components were negatively affected by the high correlated paternity of maternal plants. In Myrtus communis, high correlated paternity had a negative effect on the proportion and timing of seedling emergence in the natural field conditions and in the greenhouse sowing experiment, respectively. In Pistacia lentiscus, seedling emergence time under field conditions was also negatively influenced by high correlated paternity and a progeny survival analysis in the field experiment showed greater mortality of seedlings from maternal plants with high correlated paternity. Overall, we found effects of correlated paternity on the progeny performance of Myrtus communis, a self-compatible species. Further, we also detected effects of correlated paternity on the progeny emergence time and survival in Pistacia lentiscus, an obligate outcrossed species. This study represents one of the few existing empirical examples which highlight the influence that correlated paternity may exert on progeny performance in multiple stages during early seedling growth.

  3. High Correlated Paternity Leads to Negative Effects on Progeny Performance in Two Mediterranean Shrub Species.

    Science.gov (United States)

    Nora, Sofia; Aparicio, Abelardo; Albaladejo, Rafael G

    2016-01-01

    Anthropogenic habitat deterioration can promote changes in plant mating systems that subsequently may affect progeny performance, thereby conditioning plant recruitment for the next generation. However, very few studies yet tested mating system parameters other than outcrossing rates; and the direct effects of the genetic diversity of the pollen received by maternal plants (i.e. correlated paternity) has often been overlooked. In this study, we investigated the relation between correlated paternity and progeny performance in two common Mediterranean shrubs, Myrtus communis and Pistacia lentiscus. To do so, we collected open-pollinated progeny from selected maternal plants, calculated mating system parameters using microsatellite genotyping and conducted sowing experiments under greenhouse and field conditions. Our results showed that some progeny fitness components were negatively affected by the high correlated paternity of maternal plants. In Myrtus communis, high correlated paternity had a negative effect on the proportion and timing of seedling emergence in the natural field conditions and in the greenhouse sowing experiment, respectively. In Pistacia lentiscus, seedling emergence time under field conditions was also negatively influenced by high correlated paternity and a progeny survival analysis in the field experiment showed greater mortality of seedlings from maternal plants with high correlated paternity. Overall, we found effects of correlated paternity on the progeny performance of Myrtus communis, a self-compatible species. Further, we also detected effects of correlated paternity on the progeny emergence time and survival in Pistacia lentiscus, an obligate outcrossed species. This study represents one of the few existing empirical examples which highlight the influence that correlated paternity may exert on progeny performance in multiple stages during early seedling growth.

  4. Possibilidade de seleção recorrente para aumento do teor de óleo em soja com a utilização da macho-esterilidade genética e da espectroscopia de ressonância nuclear magnética The possibility of recurrent selection to increase the oil content in soybeans using genetic male sterility and NMR spectroscopy

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    Manoel Albino Coelho de Miranda

    1989-01-01

    Full Text Available Com a finalidade de estudar a possibilidade de realizar seleção recorrente, analisaram-se sementes de soja de plantas macho-estéreis e de sua progênies quanto ao teor de óleo no espectroscópio de ressonância nuclear magnética (NMR, no Instituto de Física da Universidade de Campinas de 1984 a 1988. Tais sementes provinham do composto IAC-1, com macho-esterilidade genética e ampla variabilidade quanto à percentagem de óleo (13,5-22,5%. Determinaram-se os coeficientes de repetibilidade, tomando-se o teor de óleo de cada semente da planta macho-estéril como uma medida fenotípica, obtendo-se o valor médio de r = 0,65. Concluiu-se que com a análise de quatro a cinco sementes, é possível representar a planta-mãe, passo importante para viabilizar a seleção recorrente nessa população, visto o pequeno número de sementes produzidas por planta macho-estéril. Utilizando-se a covariância entre a média das sementes da planta macho-estéril e a média de suas progênies, obteve-se h² = 0,73. Essa herdabilidade é alta, e como o caráter teor de óleo tem como principal componente de sua variância genética a variância aditiva, pode-se recomendar a seleção fenotípica. Calculou-se também o coeficiente de herdabilidade no nível da média de progênies, obtendo-se valores desde 0,66, para a média de duas plantas por progênie, até 0,85, para a de sete plantas por progênie. Esses resultados demonstram a possibilidade de seleção recorrente no composto IAC-1, tanto no campo, ao nível de planta macho-estéril, como em casa de vegetação, em seleção baseada na média da progênie.The seed oil content of male sterile plants and their progenies was analised by a NMR spectroscope to study the possibility of recurrent selection to improve the oil content. The seeds derived from a composite named "IAC-1" with genetic male sterility and large variability with respect to oil content. The repeatability coefficient (r for oil content

  5. Análise biométrica de ganhos por seleção em população de milho pipoca de quinto ciclo de seleção recorrente Biometric analysis of selection gains in popcorn population of the fifth cycle of recurrent selection

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    Ramon Macedo Rangel

    2011-06-01

    Full Text Available Com o intento de selecionar progênies superiores para a efetiva concentração de alelos favoráveis em uma população de quinto ciclo de seleção recorrente intrapopulacional em famílias de irmãos-completos de milho pipoca, avaliaram-se 200 famílias quanto a seis características agronômicas, incluindo rendimento de grãos e capacidade de expansão, em delineamento aleatorizado em blocos com duas repetições dentro de 'sets' em dois ambientes distintos: Colégio Estadual Agrícola Antônio Sarlo, em Campos dos Goytacazes, e Estação Experimental da PESAGRO-RIO, em Itaocara, Rio de Janeiro, em 2007/2008. Houve diferenças significativas para famílias/"sets" para todas as características, indicando a presença de variabilidade genética a ser explorada nos futuros ciclos. Na seleção das progênies superiores o índice de Mulamba e Mock resultou em ganhos proporcionais mais adequados para capacidade de expansão e rendimento de grãos, com valores respectivos de 6,01 e 8,53%, na utilização de pesos econômicos arbitrários.Aiming to select superior progenies for concentration of favorable alleles in a popcorn population on the fifth cycle of intrapopulation full-sib recurrent selection, two hundred progenies were evaluated for six agronomic traits, including grain yield and popping expansion, in randomized complete block design with two replications within sets, in two distinct environments: Colégio Estadual Agrícola Antônio Sarlo, in Campos dos Goytacazes, and Experimental Station of PESAGRO-RIO, located in Itaocara, Rio de Janeiro State, in 2007/2008. There were significant differences among families within sets in all traits, indicating the presence of genetic variability to be exploited in the future cycles. The utilized selection indexes were the Mulamba and Mock; and Smith and Hazel. The Mulamba and Mock index propitiated more elevated magnitudes of the predicted gains for most of the traits, including popping expansion and

  6. (colouration) in progeny of Poecilia reticulate (guppy)

    African Journals Online (AJOL)

    There is high demand for ornamental fishes with improve colouration. Colouration is sex specific in Poecilia reticulate (Guppy) and the colour variation within the wild and exotic breeds is at variance. The effect of parental sex and breed on colouration of progeny in Poecilia reticulata (Guppy) was studied. Two breeds of P.

  7. Number of recombinations and genetic properties of a maize population undergoing recurrent selection Número de recombinações e as propriedades genéticas de uma população de milho sob seleção recorrente

    Directory of Open Access Journals (Sweden)

    Francisco Pinheiro Lima Neto

    2009-02-01

    Full Text Available In maize recurrent selection programs, selected genotypes were recombined once to generate genetic variability for the next selection cycle. Selection generates negative gametic phase disequilibrium which reduces genetic variances, and this disequilibrium is not significantly reduced with only one generation of recombination. The objective of this research was to assess the effects of one additional generation of recombination on phenotypic and genotypic parameters in a maize population undergoing recurrent selection. Selected progenies of the EPB-4 population were subjected to one and two generations of recombination, and from each generation half- and full-sib progenies were developed and evaluated at three environments for grain yield, plant and ear heights, prolificacy, and ear placement. There were no significant changes between each progeny type with one and two generations of recombination for the means, ranges, phenotypic distribution of the traits, genetic variances, heritability coefficients, and genetic correlations for the traits assessed. The results suggest that an additional generation of recombination will not increase the effectiveness of maize recurrent selection programs.Nos programas de seleção recorrente, os genótipos selecionados são recombinados uma vez para gerar variabilidade genética para o próximo ciclo de seleção. A seleção gera desequilíbrio negativo na fase gamética, reduzindo as variâncias genéticas, e este desequilíbrio não é significantemente reduzido com apenas uma geração de recombinação. O objetivo desta pesquisa foi avaliar os efeitos de uma geração adicional de recombinação sobre parâmetros fenotípicos e genéticos em uma população de milho submetida à seleção recorrente. Progênies selecionadas da população EPB-4 foram recombinadas por uma e duas gerações, e de cada geração foram obtidas progênies de meios-irmãos e de irmãos germanos, as quais foram avaliadas em tr

  8. Selective Embolization of Systemic Collaterals for the Treatment of Recurrent Hemoptysis Secondary to the Unilateral Absence of a Pulmonary Artery in a Child

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, Yin, E-mail: zhouyin502@163.com [West China Hospital, Sichuan University, Department of Pediatric Surgery (China); Tsauo, Jiaywei, E-mail: 80732059@qq.com [West China Hospital, Sichuan University, Institute of Interventional Radiology (China); Li, Yuan, E-mail: liyuanletters@163.com [West China Hospital, Sichuan University, Department of Pediatric Surgery (China); Li, Xiao, E-mail: simonlixiao@gmail.com [West China Hospital, Sichuan University, Institute of Interventional Radiology (China)

    2015-10-15

    The unilateral absence of the pulmonary artery (UAPA) is a rare anomaly. Hemoptysis due to systemic collaterals is one of the most common complications of UAPA. To the best of the authors’ knowledge, the utility of selective embolization for the treatment of this condition in children has not been reported previously. This report describes a 6-year-old girl with isolated UAPA (IUAPA) admitted for a 10-month history of recurrent hemoptysis that had worsened during the previous 2 months. Selective embolization of the bronchial systemic collaterals was performed. The patient remained asymptomatic with no recurrence of hemoptysis 8 months after the procedure.

  9. Eficiência da seleção recorrente para redução da estatura de plantas em mamoneira (Ricinus communis L. Recurrent selection efficiency for stature reduction of castor bean (Ricinus communis L. plants

    Directory of Open Access Journals (Sweden)

    Inocencio Junior de Oliveira

    2008-08-01

    Full Text Available Realizou-se, o presente trabalho, com o objetivo de avaliar a eficiência da seleção recorrente para a redução da estatura de plantas de mamona da cultivar Guarani (Ricinus communis L., tornando-a com porte adequado para facilitar a colheita manual e/ou mecânica. Foram realizados quatro ciclos de seleção recorrente com a utilização de progênies autofecundadas na cultivar Guarani para redução da estatura das plantas, nas condições edafoclimáticas dos municípios de São Manuel - SP, Botucatu - SP e Penápolis - SP. As avaliações de estatura das plantas e de produtividade de grãos (kg.ha-1, dos quatro ciclos de seleção e do ciclo original foram realizadas nos municípios de São Manuel - SP, Botucatu - SP e Penápolis - SP na safra 2005/2006, sob um delineamento de blocos casualizados com cinco repetições e parcela útil de 30 m². A análise de variância para as características avaliadas foi feita separadamente para cada local e conjuntamente para os três locais e, posteriormente, realizada a comparação das médias pelo teste de Tukey, a 5%. Foram estimados, para as três localidades, por análise de regressão, os ganhos genéticos dos quatros ciclos de seleção para estatura de plantas. A partir dos resultados obtidos pôde-se concluir que a seleção recorrente foi eficiente para a redução da estatura de plantas e que a cultivar de mamona Guarani apresenta variabilidade genética para essa característica e que a produtividade não foi influenciada pela redução da estatura de plantas.The aim of this work was to evaluate the recurrent selection efficiency for reduction of stature of the castor bean plants of the Guarani cultivar (Ricinus communis L., turning it with appropriate strucuture to facilitate the manual and/or mechanic harvest. Four cycles of recurrent selection were accomplished through the utilization of self-pollinated progenies in the Guarani cultivar for reduction of plants stature, in

  10. Recurrent glomerular disease after kidney transplantation: an update of selected areas and the impact of protocol biopsy.

    Science.gov (United States)

    Morozumi, Kunio; Takeda, Asami; Otsuka, Yasuhiro; Horike, Keiji; Gotoh, Norihiko; Watarai, Yoshihiko

    2014-06-01

    Recurrence of native kidney disease following kidney transplantation affects between 10% and 20% of patients, and accounts for up to 8% of graft failures. In a considerable number of recipients with transplant glomerulopathy, it is impossible to distinguish between recurrent and de novo types. An accurate estimate of the incidence of recurrence is difficult due to limitations in the diagnosis of recurrent glomerulonephritis. De novo glomerular lesions may be misclassified if histological confirmation of the patient's native kidney disease is lacking. Asymptomatic histological recurrence in renal allografts may be missed if protocol biopsies are not available. Studies based on protocol biopsy are pivotal to accurately estimate the incidence of recurrence. Many factors are known to influence recurrence of kidney disease after transplantation, including the type and severity of the original disease, age at onset, interval from onset to end-stage renal disease, and clinical course of the previous transplantation. Early recognition of recurrence is possible in several glomerular diseases. Factors such as the existence of circulating permeability factors, circulating urokinase receptor and anti-phospholipase A2 receptor antibody, as well as disorders of complement regulatory proteins like factor I mutation and factor H mutation factors are expected to be useful predictors of recurrence. Peculiar clinical course of atypical haemolytic uremic syndrome after kidney transplantation is an informative sign of recurrent glomerular disease. These factors play pivotal roles in the development of recurrence of certain types of glomerulopathies. Understanding the pathogenesis of recurrent glomerulonephritis is critical to optimize prevention as well as treat individual cases of recurrent glomerulonephritis. Subclinical recurrence of IgA nephropathy after kidney transplantation is well recognized. Only protocol biopsies of clinically silent recipient can provide the accurate

  11. Selective positive-negative feedback produces the winner-take-all competition in recurrent neural networks.

    Science.gov (United States)

    Li, Shuai; Liu, Bo; Li, Yangming

    2013-02-01

    The winner-take-all (WTA) competition is widely observed in both inanimate and biological media and society. Many mathematical models are proposed to describe the phenomena discovered in different fields. These models are capable of demonstrating the WTA competition. However, they are often very complicated due to the compromise with experimental realities in the particular fields; it is often difficult to explain the underlying mechanism of such a competition from the perspective of feedback based on those sophisticate models. In this paper, we make steps in that direction and present a simple model, which produces the WTA competition by taking advantage of selective positive-negative feedback through the interaction of neurons via p-norm. Compared to existing models, this model has an explicit explanation of the competition mechanism. The ultimate convergence behavior of this model is proven analytically. The convergence rate is discussed and simulations are conducted in both static and dynamic competition scenarios. Both theoretical and numerical results validate the effectiveness of the dynamic equation in describing the nonlinear phenomena of WTA competition.

  12. Development of next-generation mapping populations: Multi-parent Advanced Generation Inter-Cross (MAGIC) and Marker-Assisted Recurrent Selection (MARS) populations in peanut

    Science.gov (United States)

    Generation Inter-Cross (MAGIC) and Marker-Assisted Recurrent Selection (MARS) have been proposed and used in many crops to dissect complex traits or QTL. MAGIC allows for dissecting genomic structure, and for improving breeding populations by integrating multiple alleles from different parents. MAR...

  13. Avaliação e seleção de progênies resultantes do cruzamento de cultivares de café Catuaí com Mundo Novo Evaluation and selection of progenies from cross of Catuaí with Mundo Novo coffee cultivars

    Directory of Open Access Journals (Sweden)

    Gladyston Rodrigues Carvalho

    2006-10-01

    Full Text Available Com o objetivo de selecionar progênies de cafeeiros resultantes do cruzamento de 'Catuaí' com 'Mundo Novo' mais produtivas e adaptadas aos diferentes ambientes, instalou-se o presente trabalho. Foram utilizadas 12 progênies, na 4ª geração por autofecundação do 2º retrocruzamento de Catuaí com Mundo, desenvolvidas pelo programa de Melhoramento Genético do Cafeeiro em Minas Gerais, coordenado pela EPAMIG e como testemunha as cultivares Catuaí Vermelho IAC-99, Rubi MG-1192 e Acaiá Cerrado MG-1474. Os experimentos foram instalados em São Sebastião do Paraíso e Três Pontas em Minas Gerais, utilizando o delineamento em blocos casualizados, com quatro repetições e seis plantas por parcela. Avaliou-se a produção de grãos em sacas de 60 kg de café beneficiado/ha. Realizou-se a análise de variância, com parcelas subdivididas onde cada colheita (ano foi considerada como uma subparcela. Os dados de produção por local foram analisados conjuntamente, considerando-se todas as colheitas. As médias dos tratamentos foram comparadas pelo teste de Scott-Knott, a 5% de probabilidade. Os resultados obtidos permitiram verificar que as melhores progênies foram H 1190-11-70-2, 1190-11-119-1, 1190-11-70-1 e 1190-11-8-2, sendo as progênies H 1190-11-70-1 e 1190-11-8-2, com maior adaptabilidade sobre os dois ambientes.The objective of this paper was to select coffee progenies from the crosses of 'Catuaí' with 'Mundo Novo', the most productives and adapted to different environment. In this experiment twelve progenies were selected, in the fourth generation for the second self-backcrossing of the Catuaí and Mundo Novo, developed by EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS, Coffee Breeding Program. The coffee cultivars Catuaí Vermelho IAC-99, Rubi MG 1192 and Acaiá Cerrado MG 1474 were also as control. The experiments were conducted in São Sebastião do Paraíso and Três Pontas in Minas Gerais using the randomized block design

  14. GENETIC VARIABILITY IN YOUNG PROGENIES OF AÇAIZEIRO

    Directory of Open Access Journals (Sweden)

    João Tomé de Farias Neto

    2005-12-01

    Full Text Available This work aimed at studying the genetic variability in young progenies of açaizeiro population. The experiment wasinstalled in the physical base of Tomé-Açu of the Embrapa Eastern Amazônia, involving the study of 25 progenies of half sibdelineated in látice 5 x 5. The experiment consisted of two repetitions and five plants per plot. Plant height (AP and diameter (DFC,number of live leaves (NFV and tillers (NP were obtained twelve months after planting. The analyses of variance showed that,except plant height and number of leaves characteristics, it had significant differences 5 % of probability for diameter plant andnumber of tillers. Values estimated in the superior extremity of the interval of variation for the characteristic appraised point outpromising individuals to be selected for producing fruits, regarding diameter of the plant, due to the fact that these characters arecorrelated positively. The biggest estimates of genetic parameters had been gotten in relation to the characteristic number of tillers,followed by plant diameter.

  15. Sudetic larch in Germany - Results of provenance and progeny research

    Energy Technology Data Exchange (ETDEWEB)

    Weisgerber, H. [Forest Centre for Management Planning, Research and Ecology, Hann Muenden (Germany)

    1995-12-31

    There are only a few older sources of Sudetic larch in Germany. They distinguish themselves by outstanding growth and low susceptibility to canker. This impression was confirmed by results of provenance research. The Sudetic larch tested in comparison with numerous other provenances proved to be fast-growing, site-tolerant, to a large extent insusceptible to canker, with straight but also slightly to moderately curved stems. The Sudetic provenances behave remarkably uniformly as regards these characteristics. In addition to provenance research investigations have been going on for a long time in Germany into individual differences within the Sudetic larch populations. A report is given on the results of progeny tests from free and controlled pollination, using the example of a seed orchard consisting of 54 clones. We point also to possibilities for improving stem quality by selection steps. The results of provenance and progeny research on Sudetic larch are in the meantime being put to use to a large extent in practical forestry. The forest administrations of various federal lands recommend the use of reproductive material of Sudetic origin and from seed orchards. 20 refs, 3 figs

  16. Avaliação e seleção de progênies F3 de cafeeiros de porte baixo com o gene SH3 de resistência a Hemileia vastatrix Berk. et Br. Evaluation and selection of Coffea arabica F3 progenies with low height and the leaf-rust SH3 resistence gene

    Directory of Open Access Journals (Sweden)

    Albano Silva da Conceição

    2005-01-01

    porte baixo portando o gene SH3 de resistência ao agente da ferrugem.The present work evaluated 36 arabic coffee (Coffea arabica L. F3 progenies, originated from crosses among cultivars Catuaí Vermelho IAC 46 and Catuaí Vermelho IAC 81 and access IAC 1110 (BA-10. This last cultivar came from India and exhibits SH2 and SH3 rust resistance genes. The experiment was installed in 1988 at the Experimental Center of the Agronomic Institute (IAC/APTA, in Campinas, using random blocks design with six repetitions and two plants per plot. Field evaluations included yield (average of seven annual harvests, vegetative vigor, resistance to leaf rust, plant size, color of young leaves and complete fruit maturation period. Based on these evaluations, plants exhibiting high yield, good vegetative vigor, low height, and resistance to the leaf rust agent Hemileia vastatrix were selected. Fruit yield of selected plants was calculated and seeds were characterized according to type (flat, peaberry and elephant, outturn and grain size. A total of 11 optimal F3 progenies were identified as rust resistant. By further classifications, 39 plants out from these progenies were selected, along with 15 plants from other 25 evaluated progenies. Laboratory analyses lead to a final selection of 18 coffee trees, all exhibiting leaf rust resistance, high yield and low height. Also, F4 progenies of selected plants had been evaluated regarding height and leaf rust resistance, at seedling stage, in greenhouse conditions. Eighteen plants were selected for further analysis and move forward from F3 to F4 generation in the coffee breeding program developed by IAC.

  17. Providing supplemental milk to piglets preweaning improves the growth but not survival of gilt progeny compared with sow progeny.

    Science.gov (United States)

    Miller, Y J; Collins, A M; Smits, R J; Thomson, P C; Holyoake, P K

    2012-12-01

    Gilt progeny have lighter weaning weights and greater postweaning medication and mortality rates compared with the progeny of older parity sows. Because weaning weight has been positively correlated with postweaning survival, this study aimed to determine whether the provision of supplemental milk preweaning could improve weaning weight and subsequent weights as well as postweaning survival of gilt progeny. The study was replicated in summer and winter as the effects of supplemental milk were expected to vary with season. The progeny of 80 gilts (parity 0) and 80 sows (parity 2 to 5) were allocated to both treatments: with or without supplemental milk in these 2 seasons with 5 sheds/season. Litter size was standardized (10 to 11 piglets) and each piglet was weighed at birth, d 21, weaning (4 wk), and 10 wk of age. Medications and mortalities were recorded both preweaning and postweaning. Pigs were housed within treatment groups postweaning, and ADFI and G:F were measured. Gilt progeny were 200 g lighter at birth in both replicates (P gilt and sow progeny by 800 g in summer (P gilts or sows (P > 0.05). Supplemental milk disappearance (the daily difference between the volume of milk provided and the residue left in the drinker) was greater in summer than winter (by 130 mL/piglet d(-1); P gilt progeny reached or exceeded that of nonsupplemented sow progeny. Gilt progeny had greater postweaning mortality (2.6%) and medication rates (6.2%) than sow progeny (1 and 2.2%, respectively; both P Gilt progeny also had less postweaning ADFI than sow progeny in winter (528 and 636 g, respectively; P 0.05). The hypothesis that supplemental milk provision did increase gilt progeny weaning weight was supported (especially in summer) but the supplementation had no effect on postweaning weights and survival. Efforts to improve gilt progeny postweaning growth and survival need to be aimed at improving health and immunity, not just weaning weight.

  18. Effect of recurrent selection on the genetic variability of the UNB-2U popcorn population using RAPD markers = Efeito da seleção recorrente na população UNB-2U de milho pipoca por marcadores RAPD

    Directory of Open Access Journals (Sweden)

    Felipe Oliveira Vilela

    2008-01-01

    Full Text Available The aim of this research was to study the effects of recurrent selection on the genetic variability of UNB-2U popcorn population after three cycles of recurrent selection (mass selection, full-sib selection and S1 families based on RAPD markers in 30 progenies from each selection cycle. There was no significant variation between the C0 and C2 cycles based on RAPD, showing that the use of different recurrent selection strategies in the cycles did not decrease genetic variability, due to the size of the population selected in the different cycles. The significant difference observed between mean values of C1 and C2 cycles was attributed to the smaller population size in C1 generation. Individuals were distributed into three large clusters and 20% of the individuals were placed in a groupdifferent from their original cycle. This can be explained by alleles’ transference from one generation to another and by the relationship between cycles.Com o objetivo de averiguar o impacto da seleção recorrente navariabilidade genética de progênies da população de milho pipoca UNB-2U, após 3 ciclos de seleção recorrente por diferentes métodos (massal, irmãos completos e famílias S1, 30 progênies de cada ciclo foram avaliadas por marcadores RAPD. Constatou-se que não houve variação molecular significativa entre os ciclos C0 e C2, revelando que o uso de diferentes estratégias de seleção recorrente não promoveu estreitamento genético, em razão do tamanho populacional selecionado nos ciclos. A diferença significativa na média entre osciclos C1 e C2 é atribuída ao menor tamanho populacional da geração C1. A distribuição dendrogrâmica dos indivíduos revelou a formação de 3 grandes grupos, sendo que 20% dos indivíduos foram alocados em grupo distinto do ciclo a que pertenciam, em razão da transferência de alelos nas subseqüentes gerações, bem como da própria semelhança entre os ciclos.

  19. Eficiência da seleção de progênies S1 e S2 de milho-pipoca, visando à produção de linhagens Efficiency in selecting S1 and S2 popcorn progenies for development of inbred lines

    Directory of Open Access Journals (Sweden)

    Aloisio Alcantara Vilarinho

    2003-01-01

    /99. Genetic and phenotypic parameters were estimated and the predicted gains were calculated according to several selection criteria. A significant genotypic variability was observed for yield and the volume expansion. Although a negative genotypic correlation has been observed among the characters, the selection indices were efficient in identification of the progenies that allowed for predicted simultaneous gains in grain yield and volume expansion. The families S2 and S3 from families S1 and S2, respectively, were evaluated in the growing season of 1999/2000. Based on the results from this evaluation, the obtained gains were calculated for each selection criteria, and the selection efficiency was evaluated. A satisfactory concordance occurred between the predicted and actual gains for the S1 progenies. The four criteria providing the highest predicted gains are the same ones that provided the four highest gains, although not in the same order. Also, those two criteria providing the lowest estimates of the gains are the ones that provided the two lowest gains, when considering popping expansion. In relation to S2 progenies, a concordance occurred concerning the direction of the changes predicted in five criteria, when the grain yield was considered, as well as in six criteria when considering CE.

  20. Multiple Recurrences in Aggressive Forms of Dupuytren?s Disease?Can Patients Benefit from Repeated Selective Fasciectomy?

    OpenAIRE

    K?nneker, S?ren; Broelsch, G. Felix; Krezdorn, Nicco; Dastagir, Khaled; Kuhbier, J?rn W.; Paprottka, Felix J.; Peter M Vogt

    2017-01-01

    Background: In Dupuytren?s disease (DD), limited fasciectomy is the mainstay of surgical therapy in patients at risk of contractures and disease recurrences. New minimally invasive treatments such as injection of collagenase clostridium histolyticum have evolved as a common tool for the preliminary treatment of Dupuytren?s contractures. However, recurrences and their therapy remain controversial. In this study, we evaluate the benefit of repeated limited fasciectomy in patients with aggressiv...

  1. Kerathocyst odontogenic tumor: Importance of selection the best treatment modality and a periodical follow-up to prevent from recurrence: A case report and literature review

    Directory of Open Access Journals (Sweden)

    Nasim Jafaripozve

    2013-01-01

    Full Text Available The keratocystic odontogenic tumor (KCOT is a relatively common oral and maxillofacial lesion with specific characteristics such us rapid growth, extension into the surrounding tissues and high rates of recurrence. Various treatment modalities have been reported. Due to the very thin and friable lining characteristic of the tumor, enucleation can be difficult undertaken and for this reason it is associated with the highest recurrence rates. A 22-year-old male referred to our clinic due to a slight expansion in the right mandible from 2 years ago. He has a history of occurrence of KCOT in this region that was treated surgically by enucleation and curettage 5 years ago. Cone beam computed tomography showed a multilocular radiolucent lesion that extended from the angle of the mandible to the symphysis. Incisional biopsy showed a KCOT recurrence that surgically treated with resection of the right mandible by continuity preservation. Selection of the best treatment modality and also a periodical lifelong follow-up is very important to reduce the rate of recurrence and morbidity of the patient.

  2. FOLIAR NUTRIENT CONTENTS AND FRUIT YIELD IN CUSTARD APPLE PROGENIES

    Directory of Open Access Journals (Sweden)

    Paulo Sérgio Lima e Silva

    2009-01-01

    Full Text Available Foliar nutrient contents are evaluated in several fruit trees with many objectives. Leaf analysis constitutes a way of evaluating the nutritional requirements of crops. Due to the positive impact that fertilizers have on crop yields, researchers frequently try to evaluate the correlations between yield and foliar nutrient contents. This work's objective was to present fruit yields from the 4th to the 6th cropping seasons, evaluate foliar nutrient contents (on the 5th cropping season, and estimate the correlations between these two groups of traits for 20 half-sibling custard apple tree progenies. The progenies were evaluated in a random block design with five replicates and four plants per plot. One hundred leaves were collected from the middle third of the canopy (in height of each of four plants in each plot. The leaves were collected haphazardly, i.e., in a random manner, but without using a drawing mechanism. In the analysis of variance, the nutrient concentrations in the leaves from plants of each plot were represented by the average of four plants in the plot. Fruit yield in the various progenies did not depend on cropping season; progeny A4 was the most productive. No Spearman correlation was found between leaf nutrient concentrations and fruit yield. Increased nutrient concentrations in the leaves were progeny-dependent, i.e., with regard to Na (progenies FE5 and JG1, Ca (progeny A4, Mg (progeny SM7, N (progeny A3, P (progeny M, and K contents (progeny JG3. Spearman's correlation was negative between Na-Mg, Na-Ca, and Mg-P contents, and positive between Mg-Ca and N-K contents.

  3. Estimation of human dose to radon progeny

    Energy Technology Data Exchange (ETDEWEB)

    Shimo, Michikuni [Gifu Coll. of Medical Technology, Sekiichi, Gifu (Japan)

    1996-12-01

    The aim of the paper is the estimation of the effective dose due to radon progeny for Japanese population. The estimation was performed by a modified UNSCEAR equation. The equation was needed the radon concentration annual occupancy time and the tidal volume on Japanese people and the dose conversion coefficient are needed. Furthermore, not only these figures but also unattached fraction and aerosol distribution data obtained in Japan and the factor related to the Japanese living style were used in the calculation. We used following figures as representative value in Japan; radon concentration: 13(6 - 25) Bq/m{sup 3} indoors and 6.7(3.5 - 13) Bq/m{sup 3} outdoors; the equilibrium factor: 0.45(0.35 - 0.57) indoors and 0.70(0.50 - 0.90) outdoors; the occupancy factor: 0.87 indoors, 0.09 outdoors and 0.04 in vehicle for male and 0.91 indoors, 0.06 outdoors and 0.03 in vehicle for female; the tidal volume: 7,000 (4,000 - 8,000) m{sup 3} for male, 6,200 (3,500 - 7,500) m{sup 3} for female. The effective doses due to radon progeny were estimated to be 0.45 mSv/y for male and 0.40 mSv/y for female, and the variance was -80 - +130%. These values were 1/2 - 1/3 as small as values shown by UNSCEAR 1993 Report and estimated by ICRP Publication 65. (author)

  4. Recurrent varicocele

    Directory of Open Access Journals (Sweden)

    Katherine Rotker

    2016-01-01

    Full Text Available Varicocele recurrence is one of the most common complications associated with varicocele repair. A systematic review was performed to evaluate varicocele recurrence rates, anatomic causes of recurrence, and methods of management of recurrent varicoceles. The PubMed database was evaluated using keywords "recurrent" and "varicocele" as well as MESH criteria "recurrent" and "varicocele." Articles were not included that were not in English, represented single case reports, focused solely on subclinical varicocele, or focused solely on a pediatric population (age <18. Rates of recurrence vary with the technique of varicocele repair from 0% to 35%. Anatomy of recurrence can be defined by venography. Management of varicocele recurrence can be surgical or via embolization.

  5. Initial Steps to inform selection of continuation cognitive therapy or fluoxetine for higher risk responders to cognitive therapy for recurrent major depressive disorder.

    Science.gov (United States)

    Vittengl, Jeffrey R; Anna Clark, Lee; Thase, Michael E; Jarrett, Robin B

    2017-07-01

    Responders to acute-phase cognitive therapy (A-CT) for major depressive disorder (MDD) often relapse or recur, but continuation-phase cognitive therapy (C-CT) or fluoxetine reduces risks for some patients. We tested composite moderators of C-CT versus fluoxetine's preventive effects to inform continuation treatment selection. Responders to A-CT for MDD judged to be at higher risk for relapse due to unstable or partial remission (N=172) were randomized to 8 months of C-CT or fluoxetine with clinical management and assessed, free from protocol treatment, for 24 additional months. Pre-continuation-treatment characteristics that in survival analyses moderated treatments' effects on relapse over 8 months of continuation-phase treatment (residual symptoms and negative temperament) and on relapse/recurrence over the full observation period's 32 months (residual symptoms and age) were combined to estimate the potential advantage of C-CT versus fluoxetine for individual patients. Assigning patients to optimal continuation treatment (i.e., to C-CT or fluoxetine, depending on patients' pre-continuation-treatment characteristics) resulted in absolute reduction of relapse or recurrence risk by 16-21% compared to the other non-optimal treatment. Although these novel results require replication before clinical application, selecting optimal continuation treatment (i.e., personalizing treatment) for higher risk A-CT responders may decrease risks of MDD relapse and recurrence substantively. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

  6. Applications of a silicon photodiode detector for radon progeny measurements

    CERN Document Server

    Voytchev, M; Chambaudet, A; Georgiev, G; Iovtchev, M

    1999-01-01

    An application of our developed silicon photodiode detector for radon progeny measurements is presented in this paper. It was determined the deposition velocity for free (3.6+-0.7)x10 sup - sup 3 m s sup - sup 1 and attached (1.0+-0.5)x10 sup - sup 5 m s sup - sup 1 fraction of short living radon progeny.

  7. Modelling radon progeny concentration variations in thermal spas.

    Science.gov (United States)

    Nikolopoulos, Dimitrios; Vogiannis, Efstratios

    2007-02-01

    Radon and its short-lived progenies (218Po, 214Pb, 214Bi and 214Po) are well known radioactive indoor pollutants identified as the major radiation burden component of the thermal spa users. Monitoring of short-lived progeny concentration is of great importance for short-term dose estimations both for bathers and working personnel. A prediction model of the short-lived progeny concentration variations was developed and applied on published data of the thermal spas of Lesvos Island. The physical procedures involved were modeled in a set of differential equations describing radon progeny concentration variations on the basis of radon measurements. Published daughter data were fitted on model predictions adjusting non-measured parameters, e.g. attachment and deposition rate constants for attached and unattached progenies. Attachment rate constants were estimated between 50 and 200 h-1 while the deposition rate constants between 0.25 and 5 h-1 for attached progenies and 0.5 and 170 h-1 for the unattached ones. In addition, unattached 218Po, 214Pb and 214Bi progenies were found to be shifted forward in respect to radon approximately 0.001 h, 0.05 h and 0.40 h respectively, while attached 218Po, 214Pb and 214Bi progenies 0.05 h, 0.45 h and 0.65 h respectively.

  8. Parental partners effects on progenies characteristics on hybridization within the Larix genus

    Energy Technology Data Exchange (ETDEWEB)

    Sindelar, J.; Frydl, J. [Forestry and Game Management Research Inst., Jiloviste (Czech Republic)

    1995-12-31

    Larch progenies of maternal clone No. 52-4-11 (Larix decidua Mill.) combined with the series of paternal clones of the same species and further of the Larix leptolepis Gord. and L. gmelini (Rupr.) Ledeb. species were evaluated on three research plots with larch (Larix sp.). Growth in height and thickness as well as stem forming were monitored at the age of 11 and 22 years. There was found out a significant share of variance falling on the action of mother, hence the existence of extra-nuclear heredity can be anticipated, particularly in interspecific hybrids. Comparing with the control in interspecific hybrids mostly luxuriant growth is displayed. On one of the studied plots there was found out the assumed growth depression in the quantitative characters of progenies originated from self-pollination. Some results of the research can be used as an auxiliary criterion in the selection of progenies and trees within the progenies for the establishment of seed orchards to produce hybrid seed of F2 generation. 24 refs, 6 figs, 6 tabs

  9. Genetic selection Pinus greggii progeny to the establishment of seed orchards Seleção genética de progênies de Pinus greggii para formação de pomares de sementes

    Directory of Open Access Journals (Sweden)

    Ananda Virgínia de Aguiar

    2010-10-01

    Full Text Available The main deterrent to the adoption of new species for operational plantations is the availability of  genetically improved seeds in sufficient amount to supply the  market. Therefore, this study was carried out with the objective to estimate genetic parameters for individual selection as a basis to transform the field trial into a seedling seed orchard. The trial contained forty seven half-sib families from five Mexican provenances, including northern and central Mexico sources. These were planted at Ponta Grossa, Paraná State, Brazil, in a
    randomized complete block design with nine  replications of five-tree linear plots in a 3 m x 3 m spacing. Height, dbh (diameter at breast height and stem form were assessed in thirteen-year old tree. Variance components were estimated by using SELEGEN-REML/BLUP software. Other procedures included prediction of breeding values and deviance analyses. The significant genetic variation among provenances as well as among and within families in all traits confirmed their high value. The southern Mexico sources showed the highest productivity. The expected progress in  improvement through just one cycle of selection was estimated in 21.6 % in wood volume.

    doi: 10.4336/2010.pfb.30.62.107
    Um dos principais impedimentos para a adoção de novas espécies para as plantações comerciais é a

    escassez de sementes geneticamente melhoradas, em quantidade suficiente para abastecer o mercado. Portanto,

    o objetivo deste estudo foi estimar parâmetros genéticos que possibilitem a realização de seleção individual em

    um teste combinado de procedências e progênies de P. greggii como base para transformar a área num pomar

    de sementes por mudas. No presente trabalho, foram avaliadas cinco procedências e quarenta e sete progênies

    de P. greggii da região norte e central do México. O experimento foi instalado

  10. The behavior of maize hybrids generated from contrasting progenies regarding the use of nitrogen

    Directory of Open Access Journals (Sweden)

    Fernando Lisboa Guedes

    2014-11-01

    Full Text Available The purpose of this study was to evaluate the performance of maize hybrids synthesized from contrasting genotypes with regard to the use of nitrogen that were selected for their performance in topcrosses. Sixty-seven S0:1 progenies derived from the germplasm bank of Ufla were evaluated in topcross combinations with two testers  at two nitrogen levels. The six progenies with the greatest tolerance and responsiveness to nitrogen (RT and the five with the least tolerance and responsiveness (RnTn were selected and, were, afterwards, crossed in a complete diallel, for a total of 55 hybrid combinations. The following genetic parameters were estimated: genetic variance among the hybrids (σ^2G, broad sense heritability in the mean of the hybrids, and selective accuracy (r^2gg. It was observed that the genetic parameters were greater in the environments with available nitrogen and that the early selection by performance in topcrosses of progenies tolerant to low N levels may not be made with high intensity. The hybrids tolerant to low N levels were obtained by crossing contrasting parents.

  11. [Selective biopsy of the sentinel lymph node in breast cancer: without axillary recurrences after a mean follow-up of 4.5 years].

    Science.gov (United States)

    Bañuelos Andrío, Luis; Rodríguez Caravaca, Gil; Argüelles Pintos, Miguel; Mitjavilla Casanova, Mercedes

    2014-01-01

    To analyze the rate of axillary recurrences (AR) in patients with early breast cancer who had not undergone an axillary node dissection (ALND) because of a negative sentinel lymph node biopsy (SLNB). The study includes 173 patients operated on for breast cancer and selective node biopsy. In 32 patients the SLNB was positive and undergone subsequent ALND. We followed up 141 patients with negative SLNB without LDN, with a median follow up of 55 months (range 74-36). The detection rate of SLN was of 99.42%. After a median follow-up of 4.5 years, there were no axillary recurrences. Two patients developed local recurrence, other two patients developed distant metastases and four patients developed a metachronous tumor. Four patients died, none of them because of breast cancer. The results obtained support the SLNB as an accurate technique in the axillary stratification of patients with breast cancer, offering in the cases of negative SLNB a safe axillary control after 4.5 year follow-up. Copyright © 2013 Elsevier España, S.L.U. and SEMNIM. All rights reserved.

  12. Recurrent selection with reduced 2,4-D amine doses results in the rapid evolution of 2,4-D herbicide resistance in wild radish (Raphanus raphanistrum L.).

    Science.gov (United States)

    Ashworth, Michael B; Walsh, Michael J; Flower, Ken C; Powles, Stephen B

    2016-11-01

    When used at effective doses, weed resistance to auxinic herbicides has been slow to evolve when compared with other modes of action. Here we report the evolutionary response of a herbicide-susceptible population of wild radish (Raphanus raphanistrum L.) and confirm that sublethal doses of 2,4-dichlorophenoxyacetic acid (2,4-D) amine can lead to the rapid evolution of 2,4-D resistance and cross-resistance to acetolactate synthase (ALS)-inhibiting herbicides. Following four generations of 2,4-D selection, the progeny of a herbicide-susceptible wild radish population evolved 2,4-D resistance, increasing the LD 50 from 16 to 138 g ha -1 . Along with 2,4-D resistance, cross-resistance to the ALS-inhibiting herbicides metosulam (4.0-fold) and chlorsulfuron (4.5-fold) was evident. Pretreatment of the 2,4-D-selected population with the cytochrome P450 inhibitor malathion restored chlorsulfuron to full efficacy, indicating that cross-resistance to chlorsulfuron was likely due to P450-catalysed enhanced rates of herbicide metabolism. This study is the first to confirm the rapid evolution of auxinic herbicide resistance through the use of low doses of 2,4-D and serves as a reminder that 2,4-D must always be used at highly effective doses. With the introduction of transgenic auxinic-herbicide-resistant crops in the Americas, there will be a marked increase in auxinic herbicide use and therefore the risk of resistance evolution. Auxinic herbicides should be used only at effective doses and with diversity if resistance is to remain a minimal issue. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  13. RELATIVE PERFORMANCE OF SELFED AND OUTCROSSED PROGENY IN IMPATIENS CAPENSIS.

    Science.gov (United States)

    Mitchell-Olds, Thomas; Waller, Donald M

    1985-05-01

    This study compares survival and growth of progeny derived from chasmogamous (CH) and cleistogamous (CL) flowers in Impatiens capensis, a forest annual. When progeny were grown in the field, CH seeds had significantly higher survival rates over winter (64% versus 56%), and the survival advantage of outcrossed progeny was not attributable to seed weight differences. No differences in seedling growth were detected. Greenhouse comparisons revealed no difference in seed survival but a 30% growth advantage to CH seedlings. We found no changes in developmental homeostasis of three leaf shape characters between inbred and outbred progeny, nor was there any difference in variability within CH and CL families. The outcrossing advantage observed in these experiments could not have been caused by avoidance of sib competition. Theory predicts that self-pollinated progenies may be more variable than outcrossed progenies if rare, recessive alleles are important contributors to genetic variances. Electrophoretic markers indicate that progeny derived from CH flowers are predominantly outcrossed (at least 54-97%). © 1985 The Society for the Study of Evolution.

  14. Comparison of expected and actual progeny growth differences in crossbred calves

    OpenAIRE

    Mahrt, Garry Sonn

    1987-01-01

    The growth performance of Angus-Polled Hereford F1 calves was compared to the expected progeny differences (EPD) estimated by the American Polled Hereford Association to evaluate the usefulness of EPD estimates for improving commercial beef production. Polled Hereford sires were selected from the high accuracy sires listed in the American Polled Hereford Association's sire summary. Four types of sires were used: 1) Sires with high yearling weight and high maternal EPD's; ...

  15. Dosimetry of radium-223 and progeny

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, D.R. [Pacific Northwest National Lab., Richland, WA (United States); Sgouros, G. [Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

    1999-01-01

    Radium-223 is a short-lived (11.4 d) alpha emitter with potential applications in radioimmunotherapy of cancer. Radium-223 can be complexed and linked to protein delivery molecules for specific tumor-cell targeting. It decays through a cascade of short-lived alpha- and beta-emitting daughters with emission of about 28 MeV of energy through complete decay. The first three alpha particles are essentially instantaneous. Photons associated with Ra-223 and progeny provide the means for tumor and normal-organ imaging and dosimetry. Two beta particles provide additional therapeutic value. Radium-223 may be produced economically and in sufficient amounts for widescale application. Many aspects of the chemistry of carrier-free isotope preparation, complexation, and linkage to the antibody have been developed and are being tested. The radiation dosimetry of a Ra-223-labeled antibody shows favorable tumor to normal tissue dose ratios for therapy. The 11.4-d half-life of Ra-223 allows sufficient time for immunoconjugate preparation, administration, and tumor localization by carrier antibodies before significant radiological decay takes place. If 0.01 percent of a 37 MBq (1 mCi) injection deposits in a one gram tumor mass, and if the activity is retained with a typical effective half-time (75 h), the absorbed dose will be 163 mGy MBq{sup {minus}1} (600 rad mCi{sup {minus}1}) administered. 49 refs., 5 figs., 2 tabs.

  16. Genotypic value in hybrid progenies of Panicum maximum Jacq.

    Directory of Open Access Journals (Sweden)

    Thiago Gomes dos Santos Braz

    Full Text Available ABSTRACT: Genetic breeding of forage plants has increasingly contributed to the release of more productive plants. In this regard, evaluating the genotypic value is essential when aiming to rank genotypes based on the mean free of environmental factors. Therefore, this study aimed to predict the genotypic value of agronomic and nutritive value characters of three progenies of Panicum maximum. Hybrids were evaluated in a clonal test in an incomplete-randomized design with three treatments (progenies 1, 2, and 3 and two replications (clones. Six harvests were performed at 25cm from the ground level throughout one year. Progeny 2 provided better results for total and leaf dry mass yield, regrowth, and height, and lower incidence of leaf spot. Progenies 1 and 3 had a better response for qualitative characters such as higher crude protein and digestibility and lower lignin and fiber content. Hybrid progenies of P. maximum have forage characters of interest for breeding, and when using ‘Mombaça’ grass as parents, the progeny stands out for leaf production and resistance to leaf spot and for ‘Tanzania’ grass as parent has resulted in better forage quality.

  17. Identification of cocoa trees combining high yield potential and resistance to diseases in segregating progenies In Ecuador

    Science.gov (United States)

    Diseases and low yielding planting material are the main factors limiting production of “fine” or “flavour” cocoa in Ecuador. This makes it necessary to develop modern varieties capable of overcoming these limitations. During the 1960s and 1970s INIAP tested several progenies from selected crosses...

  18. RECURRENT ABORTIONS

    African Journals Online (AJOL)

    recurrent abortion and intrauterine foetal deaths are not uncommon due to paucity of information on LA and its obstetrics manifestations. Our aim therefore was to determine the prevalence of LA in women with recurrent abortions in our community, with view of proffering therapeutic interventions. SUBJECTS AND METHODS.

  19. Selective arterial embolization for control of haematuria secondary to advanced or recurrent transitional cell carcinoma of the bladder.

    LENUS (Irish Health Repository)

    Halpenny, D

    2014-05-02

    Haematuria is a common symptom in patients with advanced transitional cell carcinoma of the bladder. We report our experience of selective pelvic embolization using gelfoam as an embolic agent to treat intractable haematuria in these patients.

  20. Recurrent Meningitis.

    Science.gov (United States)

    Rosenberg, Jon; Galen, Benjamin T

    2017-07-01

    Recurrent meningitis is a rare clinical scenario that can be self-limiting or life threatening depending on the underlying etiology. This review describes the causes, risk factors, treatment, and prognosis for recurrent meningitis. As a general overview of a broad topic, the aim of this review is to provide clinicians with a comprehensive differential diagnosis to aide in the evaluation and management of a patient with recurrent meningitis. New developments related to understanding the pathophysiology of recurrent meningitis are as scarce as studies evaluating the treatment and prevention of this rare disorder. A trial evaluating oral valacyclovir suppression after HSV-2 meningitis did not demonstrate a benefit in preventing recurrences. The data on prophylactic antibiotics after basilar skull fractures do not support their use. Intrathecal trastuzumab has shown promise in treating leptomeningeal carcinomatosis from HER-2 positive breast cancer. Monoclonal antibodies used to treat cancer and autoimmune diseases are new potential causes of drug-induced aseptic meningitis. Despite their potential for causing recurrent meningitis, the clinical entities reviewed herein are not frequently discussed together given that they are a heterogeneous collection of unrelated, rare diseases. Epidemiologic data on recurrent meningitis are lacking. The syndrome of recurrent benign lymphocytic meningitis described by Mollaret in 1944 was later found to be closely related to HSV-2 reactivation, but HSV-2 is by no means the only etiology of recurrent aseptic meningitis. While the mainstay of treatment for recurrent meningitis is supportive care, it is paramount to ensure that reversible and treatable causes have been addressed for further prevention.

  1. Genetic Gains in Yield and Yield Related Traits under Drought Stress and Favorable Environments in a Maize Population Improved Using Marker Assisted Recurrent Selection

    Directory of Open Access Journals (Sweden)

    Folusho Bankole

    2017-05-01

    Full Text Available The objective of marker assisted recurrent selection (MARS is to increase the frequency of favorable marker alleles in a population before inbred line extraction. This approach was used to improve drought tolerance and grain yield (GY in a biparental cross of two elite drought tolerant lines. The testcrosses of randomly selected 50 S1 lines from each of the three selection cycles (C0, C1, C2 of the MARS population, parental testcrosses and the cross between the two parents (F1 were evaluated under drought stress (DS and well watered (WW well as under rainfed conditions to determine genetic gains in GY and other agronomic traits. Also, the S1 lines derived from each selection types were genotyped with single nucleotide polymorphism (SNP markers. Testcrosses derived from C2 produced significantly higher grain field under DS than those derived from C0 with a relative genetic gain of 7% per cycle. Also, the testcrosses of S1 lines from C2 showed an average genetic gain of 1% per cycle under WW condition and 3% per cycle under rainfed condition. Molecular analysis revealed that the frequency of favorable marker alleles increased from 0.510 at C0 to 0.515 at C2, while the effective number of alleles (Ne per locus decreased from C0 (1.93 to C2 (1.87. Our results underscore the effectiveness of MARS for improvement of GY under DS condition.

  2. Genetic variation of phytochemical compounds in progenies of Ilex paraguariensis St. Hil.

    Directory of Open Access Journals (Sweden)

    Euclides Lara Cardozo Junior

    2009-01-01

    Full Text Available Mate (Ilex paraguariensis St. Hil contains phytochemical compounds capable of preventing a number of healthproblems. Knowledge on the genetic contribution to the variability in these compounds can help to obtain mate progenies withhigher levels thereof in breeding programs. The composition of triterpene saponins, methylxanthines, chlorogenic acid andthe antioxidant activity of eight mate progenies were evaluated. Significant differences among progenies were verified incontents of triterpene saponins (0.003-0.080%, caffeine (0.226-1.377%, theobromine (0.176-0.831%, and chlorogenicacid (1.344-2.031% and in antioxidant activity (31.251-51.406%. The contents of theobromine were found to be negativelycorrelated with saponins and caffeine, and caffeine with chlorogenic acid, while theobromine was positively correlated withchlorogenic acid. The heritability values for saponins (75.09%, caffeine (75.19%, theobromine (66.87%, chlorogenic acid(52.86% and antioxidant activity (67.75% indicate the possibility of genetic gain in selection for these traits.

  3. DNA alterations detected in the progeny of paternally irradiated Japanese medaka fish (Oryzias latipes).

    Science.gov (United States)

    Kubota, Y; Shimada, A; Shima, A

    1995-01-01

    A nonmammalian test system for germ-cell mutagenesis has been developed by using the Japanese medaka fish. We describe a system for detecting DNA alterations in F1 progeny descended from the gamma-irradiated male medaka that uses an arbitrarily primed polymerase chain reaction and fingerprinting. A combination of these two methods has some advantages for screening changes in genomic DNA of individual progeny because this detection system can (i) screen for mutational events before embryos with dominant lethal mutations are eliminated during development and (ii) detect DNA changes in the progeny of irradiated males without functional selection and bias, such as resistance to chemicals. DNA alterations are detected as changes in patterns (i.e., band loss and/or band gain) of DNA fingerprints of progeny descended from males whose spermatozoa or spermatids were gamma-irradiated (4.75 or 9.50 Gy). We determined the frequency of gamma-irradiation-induced band loss in arbitrarily primed polymerase chain reaction fingerprints of DNA from severely malformed embryos with dominant lethal mutations and hatched viable embryos. The frequency of band loss in both dominant lethal embryos and hatched viable embryos increased with increasing gamma-ray dose, although more so in the former. We detected a new band in the fingerprints as a heritable DNA alteration but not a viability- or phenotype-affecting DNA alteration in two viable mutants recovered after gamma-irradiation experiments. A cloned amplified fragment of the new band contained a repeated sequence of p(ATGT)n. Images Fig. 2 Fig. 3 Fig. 4 PMID:7816843

  4. Recurrent selection with reduced herbicide rates results in the rapid evolution of herbicide resistance in Lolium rigidum.

    Science.gov (United States)

    Neve, Paul; Powles, Stephen

    2005-04-01

    There has been much debate regarding the potential for reduced rates of herbicide application to accelerate evolution of herbicide resistance. We report a series of experiments that demonstrate the potential for reduced rates of the acetyl-co enzyme A carboxylase (ACCase)-inhibiting herbicide diclofop-methyl to rapidly select for resistance in a susceptible biotype of Lolium rigidum. Thirty-six percent of individuals from the original VLR1 population survived application of 37.5 g diclofop-methyl ha(-1) (10% of the recommended field application rate). These individuals were grown to maturity and bulk-crossed to produce the VLR1 low dose-selected line VLR1 (0.1). Subsequent comparisons of the dose-response characteristics of the original and low dose-selected VLR1 lines demonstrated increased tolerance of diclofop-methyl in the selected line. Two further rounds of selection produced VLR1 lines that were resistant to field-applied rates of diclofop-methyl. The LD50 (diclofop-methyl dose required to cause 50% mortality) of the most resistant line was 56-fold greater than that of the original unselected VLR1 population, indicating very large increases in mean population survival after three cycles of selection. In vitro ACCase inhibition by diclofop acid confirmed that resistance was not due to an insensitive herbicide target-site. Cross-resistance studies showed increases in resistance to four herbicides: fluazifop-P-butyl, haloxyfop-R-methyl, clethodim and imazethapyr. The potential genetic basis of the observed response and implications of reduced herbicide application rates for management of herbicide resistance are discussed.

  5. Influence of 2-(18F) fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography on recurrent ovarian cancer diagnosis and on selection of patients for secondary cytoreductive surgery

    DEFF Research Database (Denmark)

    Risum, Signe; Høgdall, Claus; Markova, Elena

    2009-01-01

    ) and to evaluate the influence of PET/CT on referral of patients with solitary recurrence to secondary cytoreductive surgery. From April 2005 to November 2007, 60 patients were consecutively included to PET/CT 68 times. The inclusion criteria were remission of 3 months or longer and recurrent OC suspected from...... physical examination, US, or increasing cancer antigen 125 (CA125) level (>50 U/mL or >15% above baseline level). Recurrent OC was diagnosed 58 times in 52 patients. The sensitivities of US, CT, and PET/CT for diagnosing recurrence were 66% (P = 0.003), 81% (P = 0.0001), and 97% (P ..., prospective clinical trials are needed to specify the characteristics of patients most likely to undergo complete secondary surgery and to further clarify the role of PET/CT in selecting patients for secondary surgery....

  6. Current international intercomparison measurement on radon and its progeny

    Energy Technology Data Exchange (ETDEWEB)

    Yamasaki, Keizo [Kyoto Univ., Kumatori, Osaka (Japan). Research Reactor Inst.

    1996-12-01

    The international intercomparison measurement on radon and its progeny was held between the EML of USDOE and several Japanese organisations, using the radon test chamber installed in EML. Japanese results of radon concentration by the active method using the ionization chamber or scintillation cell and the passive method using the solid track detector were about 5% small compared to that of EML. On the results of radon progeny, there were not any large systematic differences between EML and Japanese participants in spite of wide range of deviation except for the results at the condition of low aerosol density. (author)

  7. [Development of progeny after antenatal exposure to lithium oxybutyrate].

    Science.gov (United States)

    Smol'nikova, N M; Allakhverdiev, V D; Liubimov, B I

    1985-01-01

    Experiments on rats were made to study the effect of lithium hydroxybutyrate and to compare it with lithium carbonate effect on the development of the progeny after antenatal exposure to the drugs. It was established that the lithium salts under study did not produce any conclusive effect on the postnatal development of young rats. The activating component was discovered to be predominant in the action of lithium hydroxybutyrate, whereas the action of lithium carbonate was marked by the inhibitory component. As regards the effect on the development of the progeny, lithium hydroxybutyrate was less hazardous than lithium carbonate.

  8. History of northern corn leaf blight disease in the seventh cycle of recurrent selection of an UENF-14 popcorn population

    Directory of Open Access Journals (Sweden)

    Rodrigo Moreira Ribeiro

    2016-09-01

    Full Text Available To investigate the genetic resistance to northern corn leaf blight (Exserohilum turcicum disease in the UENF-14 popcorn population and the inclusion of this trait in the analysis, two hundred ten popcorn half-sib families were evaluated using an incomplete block design with treatments arranged in replicates "Sets". Regarding the Families within "Set" (F/S source of variation, enough genetic variability was found for northern corn leaf blight disease resistance to be explored in the next cycles of the UENF popcorn breeding program. The open-pollinated UENF-14 variety has a source of resistance to this disease in its genetic basis, given that it originated from crossing with an American cultivar with yellow grains that is resistant to disease. These results strengthen the possibility of selecting resistant families across the cycles, so it is concluded that the selection was effective at maintaining the balance in the occurrence of the disease in the study population and that the source of resistance is not lost with advancing cycles. The addition of the foliar disease variable in the analysis is of the utmost importance for the improvement of popcorn as it makes it possible to aggregate genes for resistance to this disease along with agronomic traits of interest.

  9. Exposure-response relationship of AMG 386 in combination with weekly paclitaxel in recurrent ovarian cancer and its implication for dose selection.

    Science.gov (United States)

    Lu, Jian-Feng; Rasmussen, Erik; Karlan, Beth Y; Vergote, Ignace B; Navale, Lynn; Kuchimanchi, Mita; Melara, Rebeca; Stepan, Daniel E; Weinreich, David M; Sun, Yu-Nien

    2012-05-01

    To characterize exposure-response relationships of AMG 386 in a phase 2 study in advanced ovarian cancer for the facilitation of dose selection in future studies. A population pharmacokinetic model of AMG 386 (N = 141) was developed and applied in an exposure-response analysis using data from patients (N = 160) with recurrent ovarian cancer who received paclitaxel plus AMG 386 (3 or 10 mg/kg once weekly) or placebo. Reduction in the risk of progression or death with increasing exposure (steady-state area under the concentration-versus-time curve [AUC(ss)]) was assessed using Cox regression analyses. Confounding factors were tested in multivariate analysis. Alternative AMG 386 doses were explored with Monte Carlo simulations using population pharmacokinetic and parametric survival models. There was a trend toward increased PFS with increased AUC(ss) (hazard ratio [HR] for each one-unit increment in AUC(ss), 0.97; P = 0.097), suggesting that the maximum effect on prolonging PFS was not achieved at the highest dose tested (10 mg/kg). Among patients with AUC(ss) ≥ 9.6 mg h/mL, PFS was 8.1 months versus 5.7 months for AUC(ss) AMG 386 15 mg/kg once weekly would result in an AUC(ss) ≥ 9.6 mg h/mL in > 90% of patients with median PFS of 8.2 months versus 5.0 months for placebo (HR [15 mg/kg vs. placebo], 0.56). Increased exposure to AMG 386 was associated with improved clinical outcomes in recurrent ovarian cancer, supporting the evaluation of a higher dose in future studies.

  10. Selection against recombinant hybrids maintains reproductive isolation in hybridizing Populus species despite F1 fertility and recurrent gene flow.

    Science.gov (United States)

    Christe, Camille; Stölting, Kai N; Bresadola, Luisa; Fussi, Barbara; Heinze, Berthold; Wegmann, Daniel; Lexer, Christian

    2016-06-01

    Natural hybrid zones have proven to be precious tools for understanding the origin and maintenance of reproductive isolation (RI) and therefore species. Most available genomic studies of hybrid zones using whole- or partial-genome resequencing approaches have focused on comparisons of the parental source populations involved in genome admixture, rather than exploring fine-scale patterns of chromosomal ancestry across the full admixture gradient present between hybridizing species. We have studied three well-known European 'replicate' hybrid zones of Populus alba and P. tremula, two widespread, ecologically divergent forest trees, using up to 432 505 single-nucleotide polymorphisms (SNPs) from restriction site-associated DNA (RAD) sequencing. Estimates of fine-scale chromosomal ancestry, genomic divergence and differentiation across all 19 poplar chromosomes revealed strikingly contrasting results, including an unexpected preponderance of F1 hybrids in the centre of genomic clines on the one hand, and genomically localized, spatially variable shared variants consistent with ancient introgression between the parental species on the other. Genetic ancestry had a significant effect on survivorship of hybrid seedlings in a common garden trial, pointing to selection against early-generation recombinants. Our results indicate a role for selection against recombinant genotypes in maintaining RI in the face of apparent F1 fertility, consistent with the intragenomic 'coadaptation' model of barriers to introgression upon secondary contact. Whole-genome resequencing of hybridizing populations will clarify the roles of specific genetic pathways in RI between these model forest trees and may reveal which loci are affected most strongly by its cyclic breakdown. © 2016 John Wiley & Sons Ltd.

  11. Estimativa de parâmetros genéticos e ganhos na seleção para caracteres de crescimento em teste de progênies de Pinus caribaea var. hondurensis e var. bahamensis, em Assis-SP. Estimates of genetic parameters and gains from selection for growth traits in Pinus caribaea var. hondurensis and var. bahamensis progeny tests, in Assis-SP.

    Directory of Open Access Journals (Sweden)

    Alexandre Magno SEBBENN

    2010-12-01

    Full Text Available Os objetivos deste estudo foram estimar parâmetros genéticos e ganhos na seleção em diâmetro à altura do peito – DAP e altura em um teste de 14 progênies de polinização aberta de P. caribaea var. hondurensis e seis de P. caribaea var. bahamensis, aos 13 anos de idade, estabelecido em Assis, SP. Para fins de comparação, uma testemunha P. caribaea var. hondurensis foi incluída no ensaio. O delineamento experimental utilizado foi o látice retangular 4 x 5, com parcelas de dez plantas no espaçamento de 3 m x 3 m, totalizando 20 tratamentos com três repetições. Não foram detectadas diferenças entre as variedades. Variações significativas entre progênies de ambas asvariedades foram detectadas em altura. As médias das progênies de ambas as variedades foram maiores que a média da testemunha, indicando que o material testado tem potencial para ser utilizado em reflorestamentos na região de Assis. A variação genética e os coeficientes de herdabilidades foram maiores em DAP do que em altura, indicando que maiores ganhos podem ser obtidos mediante seleção em DAP. Os ganhos genéticos preditos em plantios com 13 anos de idade, em locais com características ambientais semelhantes às de Assis e realizados com sementes coletadas após a seleção no teste de progênies de Pinus caribaea var. bahamensis foi estimado para o DAP em 6,36%.The aims of this study were to estimate genetic parameters and gains from selection for diameter at breast height – DBH and height in 14 open-pollinated progenies of P. caribaea var. hondurensis and six of P. caribaea var. bahamensis, at 13 years of age, established in Assis, State of São Paulo. A control treatment with the same var. bahamensis was included in the trial. The trial was analyzed as a 4 x 5 rectangular lattice, with 21 treatments, three replications and 10 plants per plot. No significant differences were found between varieties. A significant variation among progenies within

  12. Segregation and expression of transgenes in the progenies of Bt ...

    African Journals Online (AJOL)

    DR. NJ TONUKARI

    2012-04-17

    Apr 17, 2012 ... rent progenies of Bt transgenic rice crosses to conventional rice varieties was analyzed by Western dot blotting. The samples derived from GUS positive plants of. Bt transgenic rice crossed to conventional rice varieties were found to produce a higher level of toxin protein, but with greater range of variation.

  13. The study of triploid progenies crossed between different ploidy ...

    African Journals Online (AJOL)

    The study of triploid progenies crossed between different ploidy grapes. L Sun, G Zhang, A Yan, H Xu. Abstract. The cross between different ploidy grape was one of the effective ways to obtain new seedless cultivars, in this study, through testing the changes of the ovule weight and observing its anatomical structure, the ...

  14. Adversity before Conception Will Affect Adult Progeny in Rats

    Science.gov (United States)

    Shachar-Dadon, Alice; Schulkin, Jay; Leshem, Micah

    2009-01-01

    The authors investigated whether adversity in a female, before she conceives, will influence the affective and social behavior of her progeny. Virgin female rats were either undisturbed (controls) or exposed to varied, unpredictable, stressors for 7 days (preconceptual stress [PCS]) and then either mated immediately after the end of the stress…

  15. Segregation and expression of transgenes in the progenies of Bt ...

    African Journals Online (AJOL)

    PCR, Southern blotting and Western dot blotting analysis confirmed that cry1Ab gene was transferred to the genome of conventional rice varieties and it was highly expressed in the different progenies of Bt rice crossed to conventional rice varieties. Among these lines, the highest Bt toxin protein content reached 2.88% of ...

  16. Thoron and thoron progeny measurements in German clay houses.

    Science.gov (United States)

    Gierl, S; Meisenberg, O; Feistenauer, P; Tschiersch, J

    2014-07-01

    In recent years, elevated thoron concentrations were found in houses built of unfired clay. In this study experiments were carried out in 17 traditional and modern clay houses in Germany to obtain an overview of indoor thoron in such houses. Long-term measurements over an 8-week period were performed using a newly developed Unattended Battery-Operated Progeny Measurement Device (UBPM) for measuring thoron progeny. This instrument uses a high-voltage electric field to precipitate radon and thoron progeny on nuclear track detectors. Additional active and passive measurements of radon, thoron and their progeny were performed. The equilibrium equivalent thoron concentration was found to be between 2 and 10 Bq m(-3). Gas concentrations were found to be between 20 and 160 Bq m(-3) for radon and between 10 and 90 Bq m(-3) for thoron 20 cm from the wall. The thoron exposure contributes significantly to the inhalation dose of the dwellers (0.6-4 mSv a(-1)). © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  17. Recurrent pericarditis.

    Science.gov (United States)

    Imazio, M; Battaglia, A; Gaido, L; Gaita, F

    2017-05-01

    Recurrent pericarditis is the most troublesome complication of pericarditis occurring in 15 to 30% of cases. The pathogenesis is often presumed to be immune-mediated although a specific rheumatologic diagnosis is commonly difficult to find. The clinical diagnosis is based on recurrent pericarditis chest pain and additional objective evidence of disease activity (e.g. pericardial rub, ECG changes, pericardial effusion, elevation of markers of inflammation, and/or imaging evidence of pericardial inflammation by CT or cardiac MR). The mainstay of medical therapy for recurrent pericarditis is aspirin or a non-steroidal anti-inflammatory drug (NSAID) plus colchicine. Second-line therapy is considered after failure of such treatments and it is generally based on low to moderate doses of corticosteroids (e.g. prednisone 0.2 to 0.5 mg/kg/day or equivalent) plus colchicine. More difficult cases are treated with combination of aspirin or NSAID, colchicine and corticosteroids. Refractory cases are managed by alternative medical options, including azathioprine, or intravenous human immunoglobulins or biological agents (e.g. anakinra). When all medical therapies fail, the last option may be surgical by pericardiectomy to be recommended in well-experienced centres. Despite a significant impairment of the quality of life, the most common forms of recurrent pericarditis (usually named as "idiopathic recurrent pericarditis" since without a well-defined etiological diagnosis) have good long-term outcomes with a negligible risk of developing constriction and rarely cardiac tamponade during follow-up. The present article reviews current knowledge on the definition, diagnosis, aetiology, therapy and prognosis of recurrent pericarditis with a focus on the more recent available literature. Copyright © 2016 Société Nationale Française de Médecine Interne (SNFMI). Published by Elsevier SAS. All rights reserved.

  18. Surveillance for cancer recurrence in long-term young breast cancer survivors randomly selected from a statewide cancer registry.

    Science.gov (United States)

    Jones, Tarsha; Duquette, Debra; Underhill, Meghan; Ming, Chang; Mendelsohn-Victor, Kari E; Anderson, Beth; Milliron, Kara J; Copeland, Glenn; Janz, Nancy K; Northouse, Laurel L; Duffy, Sonia M; Merajver, Sofia D; Katapodi, Maria C

    2018-01-20

    This study examined clinical breast exam (CBE) and mammography surveillance in long-term young breast cancer survivors (YBCS) and identified barriers and facilitators to cancer surveillance practices. Data collected with a self-administered survey from a statewide, randomly selected sample of YBCS diagnosed with invasive breast cancer or ductal carcinoma in situ younger than 45 years old, stratified by race (Black vs. White/Other). Multivariate logistic regression models identified predictors of annual CBEs and mammograms. Among 859 YBCS (n = 340 Black; n = 519 White/Other; mean age = 51.0 ± 5.9; diagnosed 11.0 ± 4.0 years ago), the majority (> 85%) reported an annual CBE and a mammogram. Black YBCS in the study were more likely to report lower rates of annual mammography and more barriers accessing care compared to White/Other YBCS. Having a routine source of care, confidence to use healthcare services, perceived expectations from family members and healthcare providers to engage in cancer surveillance, and motivation to comply with these expectations were significant predictors of having annual CBEs and annual mammograms. Cost-related lack of access to care was a significant barrier to annual mammograms. Routine source of post-treatment care facilitated breast cancer surveillance above national average rates. Persistent disparities regarding access to mammography surveillance were identified for Black YBCS, primarily due to lack of access to routine source of care and high out-of-pocket costs. Public health action targeting cancer surveillance in YBCS should ensure routine source of post-treatment care and address cost-related barriers. Clinical Trials Registration Number: NCT01612338.

  19. Experimental alternatives for evaluation of progenies and clones in eucalyptus breeding programs

    Directory of Open Access Journals (Sweden)

    Souza Elaine Aparecida de

    2003-01-01

    Full Text Available The feasibility of using augmented block designs and spatial analysis methods for early stage selection in eucalyptus breeding programs was tested. A total of 113 half-sib progenies of Eucalyptus urophylla and eight clones were evaluated in an 11 x 11 triple lattice experiment at two locations: Posto da Mata (Bahia, Brazil and São Mateus (Minas Gerais, Brazil. Four checks were randomly allocated within each block. Plots consisted of 15 m long rows containing 6 plants spaced 3 m apart. The girth at breast height (cm/plant was evaluated at 19 and 26 months of age. Variance analyses were performed according to the following methods: lattice design, randomized complete block design, augmented block design, Papadakis method, moving means method, and check plots. Comparisons among different methods were based on the magnitude of experimental errors and precision of the estimates of genetic and phenotypic parameters. General results indicated that augmented block design is useful to evaluate progenies and clones in early selection in eucalyptus breeding programs using moderate and low selection intensities. However, this design is not suitable for estimating genetic and phenotypic parameters due to its low precision. Check plots, nearest neighbour, Papadakis (1937, and moving means methods were efficient in removing the heterogeneity within blocks. These efficiencies were compared to that in lattice analysis for estimation of genetic and phenotypic parameters.

  20. Growth variation and heritability in a second-generation Eucalyptus urophylla progeny test at Lad Krating Plantation, Chachoengsao province, Thailand

    Directory of Open Access Journals (Sweden)

    Lucky Nhlanhla Dlamini

    2017-06-01

    Full Text Available In Thailand, Eucalyptus urophylla was introduced with the main purpose of supplying raw material for pulp and chip wood production. The demand for genetically improved seed is increasing to support high productivity plantation establishment. One of the tree improvement activities established to meet the high demand for improved seed was a second generation progeny test at Lad Krating Plantation, Thailand to provide the best material for the successful plantation program. The aim of the current study was to compare growth variation of the first and second generation of Eucalyptus urophylla progeny that could provide information on suitable families for improved quality seed. The progeny test comprised the best 45 half-sib families selected from 80 half-sib families of the first-generation progeny test. The design of the progeny test was a randomized and complete block design (16 trees/plot × 45 plots/block × 9 blocks, with 4 rows of 4 trees at a spacing of 2 m × 1 m. Growth was assessed at age 3 yr. The average height and diameter at breast height over bark (DBH, was 13.72 m, and 8.75 cm, respectively. There were highly significant (p < 0.01 differences among provenances and families in both height and DBH. The individual heritability values for height and DBH were 0.48 and 0.60, respectively. The family heritability values for height and DBH were 0.98 and 0.99, respectively. These 45 half-sib families proved to be genetically superior ensuring higher productivity and contributing to the success of the Forest Industry Organization plantation at Lad Krating.

  1. Associação entre características agronômicas e capacidade de expansão em população de milho pipoca sob seleção recorrente Association between agronomical traits and popping expansion in a popcorn population under recurrent selection

    Directory of Open Access Journals (Sweden)

    Ramon Macedo Rangel

    2011-04-01

    Full Text Available Objetivou-se, neste trabalho, investigar a possibilidade de seleção mais eficiente por meio do uso de análise de trilha e de correlações parciais no programa de seleção recorrente da população UENF de milho pipoca. Duzentas famílias de irmãos completos foram obtidas e avaliadas quanto a oito características em dois ambientes no estado do Rio de Janeiro: Campos dos Goytacazes e Itaocara. A correlação genotípica entre capacidade de expansão e rendimento de grãos foi negativa e não significativa ao nível de 5% de probabilidade pelo teste t. A análise de trilha demonstrou ser a massa de 100 grãos, a característica mais associada à capacidade de expansão neste estudo. Há possibilidade de obtenção de resposta correlacionada em capacidade de expansão e rendimento de grãos, desde que se selecionem, entre os genótipos de maior rendimento, aqueles com menores tamanhos de grãos.The objective of this work was to investigate the possibility of a more efficient selection through path analysis and partial correlation in the breeding program of the UENF popcorn population by recurrent selection. Two hundred full-sib progenies were obtained and evaluated by eight traits in two environments in Rio de Janeiro State: Campos dos Goytacazes and Itaocara. The genotypic correlation between popping expansion and grain yield was negative and non significant at the 5% probability level by t test. Path analysis showed that mass weight of 100 grains is the most associated trait at popping expansion in this study. It is possible to obtain correlated response for popping expansion by grain yield, as long as genotypes with smaller grain size are selected from the genotypes with higher grain yield.

  2. Recent advances in Progeny testing program for Nili Ravi buffalo in Pakistan

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    A. Ghaffar

    2010-02-01

    Full Text Available Progeny testing program was started in Nili-Ravi buffalo at Livestock Production and Research Institute (LPRI, Bahadurnagar, Okara in 1979-80. Initially buffalo bulls used at Livestock Experiment Station (LES Qadirabad and Rakh Gulaman during 1964-78 were evaluated. Five out of ten at Rakh Gulaman and 10 out of 22 at Qadirabad bulls were positive. As none of these bulls were available, therefore, sons and grandsons of these bulls were selected for production of future candidate bulls. All the buffaloes at LPRI were evaluat`ed on Most Probable Producing Ability. The elite herd thus formed was used for production for future candidate bulls. Since then a continuous program is in operation at Govt. Livestock Farms and expanded to 27 field sub-centers involving private farmers in four districts Okara, Sahiwal, Faisalabad and Pakpattan. Since the initiation of this program in 1980’s, about 174 bulls have been put under progeny testing program and 137 bulls were evaluated by Daughter-Dam Comparison, out of which sixty eight buffalo bulls contributed positively in milk production of daughters. It is worth mentioning here that milk production of registered buffalo was recorded on monthly intervals in the field along with other necessary reproduction data required to compute the different traits of economic importance.Progeny testing program was started in Nili-Ravi buffalo at Livestock Production and Research Institute (LPRI, Bahadurnagar, Okara in 1979-80. Initially buffalo bulls used at Livestock Experiment Station (LES Qadirabad and Rakh Gulaman during 1964-78 were evaluated. Five out of ten at Rakh Gulaman and 10 out of 22 at Qadirabad bulls were positive. As none of these bulls were available, therefore, sons and grandsons of these bulls were selected for production of future candidate bulls. All the buffaloes at LPRI were evaluat`ed on Most Probable Producing Ability. The elite herd thus formed was used for production for future candidate bulls

  3. Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa

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    Rekiya O. Abdulmalik

    2017-05-01

    Full Text Available Marker-assisted recurrent selection (MARS is a breeding method used to accumulate favorable alleles that for example confer tolerance to drought in inbred lines from several genomic regions within a single population. A bi-parental cross formed from two parents that combine resistance to Striga hermonthica with drought tolerance, which was improved through MARS, was used to assess changes in the frequency of favorable alleles and its impact on inbred line improvement. A total of 200 testcrosses of randomly selected S1 lines derived from the original (C0 and advanced selection cycles of this bi-parental population, were evaluated under drought stress (DS and well-watered (WW conditions at Ikenne and under artificial Striga infestation at Abuja and Mokwa in Nigeria in 2014 and 2015. Also, 60 randomly selected S1 lines each derived from the four cycles (C0, C1, C2, C3 were genotyped with 233 SNP markers using KASP assay. The results showed that the frequency of favorable alleles increased with MARS in the bi-parental population with none of the markers showing fixation. The gain in grain yield was not significant under DS condition due to the combined effect of DS and armyworm infestation in 2015. Because the parents used for developing the bi-parental cross combined tolerance to drought with resistance to Striga, improvement in grain yield under DS did not result in undesirable changes in resistance to the parasite in the bi-parental maize population improved through MARS. MARS increased the mean number of combinations of favorable alleles in S1 lines from 114 in C0 to 124 in C3. The level of heterozygosity decreased by 15%, while homozygosity increased by 13% due to the loss of some genotypes in the population. This study demonstrated the effectiveness of MARS in increasing the frequency of favorable alleles for tolerance to drought without disrupting the level of resistance to Striga in a bi-parental population targeted as a source of improved

  4. Homoeologous GSL-ELONG gene replacement for manipulation of aliphatic glucosinolates in Brassica rapa L. by marker assisted selection

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    Arvind H. Hirani

    2013-03-01

    Full Text Available Aliphatic glucosinolates are the predominant sulphur-rich plant secondary metabolites in economically important Brassica crops. Glucosinolates and their hydrolysis products are involved in plant-microbe, plant-insect, plant-animal and plant-human interactions. It is, therefore, important to manipulate glucosinolate profiles and contents in Brassica species. In this study, aliphatic glucosinolates were genetically manipulated through homoeologous recombination in backcross lines followed by marker assisted selection in B. rapa. A resynthesized B. napus line, from a cross between B. rapa and B. oleracea, was backcrossed with Chinese cabbage doubled haploid line, RI16. Marker assisted selection for non-functional gene was performed in each backcross generations. Advanced backcross progenies (BC3F2 were developed to identify homoeologous gene replacement and/or introgression. Reduction in 5C aliphatic glucosinolates (gluconapoleiferin, glucoalyssin and glucobrassicanapin was observed in BC3F2 progenies of the recurrent parent that carried the GSL-ELONG gene. The GSL-ELONG positive backcross progenies were also screened by the A-genome and BraGSL-ELONG gene specific marker, which linked with 5C aliphatic glucosinolates. The A-genome specific marker was absent in the plants of advanced backcross progenies which showed reduction in 5C aliphatic glucosinolates. The results suggest that the functional allele had been replaced by the non-functional GSL-ELONG allele from B. oleracea. Some advanced backcross progenies (BC3F2 positive for the GSL-ELONG allele and the A-genome specific SCAR marker BraMAM1-1 did not show reduction in 5C aliphatic glucosinolates, suggesting that GSL-ELONG allele is recessive. Replacement of the functional locus in the A genome by non-functional counterpart in the C genome reduced the content of 5C aliphatic glucosinolates in B. rapa seeds with 20 micromoles per gram.

  5. Segregation and Heritability of Male Sterility in Populations Derived from Progeny of Satsuma Mandarin.

    Science.gov (United States)

    Goto, Shingo; Yoshioka, Terutaka; Ohta, Satoshi; Kita, Masayuki; Hamada, Hiroko; Shimizu, Tokurou

    2016-01-01

    Male sterility derived from Satsuma mandarin (Citrus unshiu) has been used in Japanese citrus breeding programs to obtain seedless cultivars, which is a desirable trait for consumers. Male sterility has often been evaluated by anther development or pollen fertility; however, the inheritance and heritability of male sterility derived from Satsuma is poorly understood. In this study, we investigated the mode of inheritance and broad-sense heritability of male sterility derived from Satsuma. Initially, we evaluated the total number of pollen grains per anther and apparent pollen fertility, as indicated by lactophenol blue staining, in 15 citrus cultivars and selections to understand the male sterility of Satsuma. The results indicated that male sterility was primarily caused by decreased number of pollen grains per anther in progeny of Satsuma. We also evaluated these traits in three F1 populations (hyuganatsu × 'Okitsu No. 56', 'Okitsu No. 46' × 'Okitsu No. 56' and 'Okitsu No. 46' × 'Kara'), of which the parents are derived from Satsuma. Individuals in these populations showed strong segregation for number of pollen grains per anther. The apparent fertility of pollen also showed segregation but was almost constant at 70%-90%. The estimated broad-sense heritability for the number of pollen grains per anther was as high as 0.898 in the 'Okitsu No. 46' × 'Okitsu No. 56' and 'Okitsu No. 46' × 'Kara' populations. These results indicated that the number of pollen grains per anther primarily determined male sterility among progeny of Satsuma, and this trait was inherited by the progeny. Development of DNA markers closely linked to male sterility using the F1 populations of 'Okitsu No. 46' × 'Okitsu No. 56' and 'Okitsu No. 46' × 'Kara' is expected to contribute to the breeding of novel seedless citrus cultivars.

  6. Segregation and Heritability of Male Sterility in Populations Derived from Progeny of Satsuma Mandarin.

    Directory of Open Access Journals (Sweden)

    Shingo Goto

    Full Text Available Male sterility derived from Satsuma mandarin (Citrus unshiu has been used in Japanese citrus breeding programs to obtain seedless cultivars, which is a desirable trait for consumers. Male sterility has often been evaluated by anther development or pollen fertility; however, the inheritance and heritability of male sterility derived from Satsuma is poorly understood. In this study, we investigated the mode of inheritance and broad-sense heritability of male sterility derived from Satsuma. Initially, we evaluated the total number of pollen grains per anther and apparent pollen fertility, as indicated by lactophenol blue staining, in 15 citrus cultivars and selections to understand the male sterility of Satsuma. The results indicated that male sterility was primarily caused by decreased number of pollen grains per anther in progeny of Satsuma. We also evaluated these traits in three F1 populations (hyuganatsu × 'Okitsu No. 56', 'Okitsu No. 46' × 'Okitsu No. 56' and 'Okitsu No. 46' × 'Kara', of which the parents are derived from Satsuma. Individuals in these populations showed strong segregation for number of pollen grains per anther. The apparent fertility of pollen also showed segregation but was almost constant at 70%-90%. The estimated broad-sense heritability for the number of pollen grains per anther was as high as 0.898 in the 'Okitsu No. 46' × 'Okitsu No. 56' and 'Okitsu No. 46' × 'Kara' populations. These results indicated that the number of pollen grains per anther primarily determined male sterility among progeny of Satsuma, and this trait was inherited by the progeny. Development of DNA markers closely linked to male sterility using the F1 populations of 'Okitsu No. 46' × 'Okitsu No. 56' and 'Okitsu No. 46' × 'Kara' is expected to contribute to the breeding of novel seedless citrus cultivars.

  7. Recurrent Stroke

    DEFF Research Database (Denmark)

    Andersen, Søren Due; Gorst-Rasmussen, Anders; Lip, Gregory Y H

    2015-01-01

    Background and Purpose—The CHA2DS2VASc score and the Essen Stroke Risk Score are respectively used for risk stratification in patients with atrial fibrillation and in patients with cerebrovascular incidents. We aimed to test the ability of the 2 scores to predict stroke recurrence, death......, and cardiovascular events (stroke, transient ischemic attack, myocardial infarction, or arterial thromboembolism) in a nationwide Danish cohort study, among patients with incident ischemic stroke and no atrial fibrillation. Methods—We conducted a registry-based study in patients with incident ischemic stroke...... and no atrial fibrillation. Patients were stratified according to the CHA2DS2VASc score and the Essen Stroke Risk Score and were followed up until stroke recurrence or death. We estimated stratified incidence rates and hazard ratios and calculated the cumulative risks. Results—42 182 patients with incident...

  8. Behavior of radon progeny in low frequency electromagnetic fields

    CERN Document Server

    Oda, K; Yamamoto, T

    1999-01-01

    Whether the electro-magnetic (EM) fields are carcinogenic or not still remains to be discussed from scientific point of view. Recently a possibility was pointed out that increased deposition of radon progeny in the EM-fields should enhance exposure dose to internal body. We investigated the behavior of charged sup 2 sup 2 sup 2 Rn progeny and aerosols containing them by measuring the pattern and the magnitude of the deposition rate of decay products on both CR-39 track detectors and imaging plates under various conditions. We concluded that the attachment to wire cables should be increased mainly by electric component of low frequency EM-fields and possibly by electric field induced by strong changing magnetic ones.

  9. Genetic progress of phenotypic recurrent selection in popcorn Progresso genético da seleção recorrente fenotípica em milho-pipoca

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    Hugo Junqueira Ematné

    2012-02-01

    Full Text Available Popcorn is widely consumed in the south of Minas Gerais; however, up to now no specific breeding program for the region has been carried out. Recurrent selection (RS is a viable alternative for breeding of this crop; nevertheless, it is necessary to frequently evaluate the progress achieved. Consequently, the purpose of this work was to assess the genetic progress obtained after two cycles of RS for the traits of popping expansion (PE and grain yield (GY. Cycles one and two of the UFLA - E and UFLA - R populations, the original population (UFLA and two controls were assessed. The experiment was conducted at the UFLA experimental farm in a randomized block design with eleven replications; ten agronomic traits, including PE and GY, were assessed. Gains from selection for the traits studied were observed, as well as that the type of kernel had an influence on the response for genetic progress for the PE trait and that the populations assessed exhibited potential for future selection cycles.O milho-pipoca é amplamente consumido no Sul de Minas Gerais, entretanto, até o momento, nenhum programa de melhoramento especifico para a região foi conduzido. A seleção recorrente (RS é uma alternativa viável para o melhoramento da cultura, todavia faz-se necessário verificar frequentemente o progresso obtido. Por conseguinte, objetivou-se avaliar o progresso genético obtido após dois ciclos de RS para os caracteres capacidade de expansão (PE e rendimento de grãos (GY. Foram avaliados os ciclos um e dois das populações UFLA - E e UFLA - R, a população original (UFLA e duas testemunhas. O experimento foi conduzido na fazenda experimental da UFLA, no delineamento em blocos ao acaso com onze repetições, dez características agronômicas incluindo PE e GY foram avaliadas. Constataram-se ganhos com a seleção para os caracteres estudados, que o tipo de grão (pérola e espinho influenciou na resposta do progresso genético para o caráter PE e que

  10. Progeny Clustering: A Method to Identify Biological Phenotypes

    Science.gov (United States)

    Hu, Chenyue W.; Kornblau, Steven M.; Slater, John H.; Qutub, Amina A.

    2015-01-01

    Estimating the optimal number of clusters is a major challenge in applying cluster analysis to any type of dataset, especially to biomedical datasets, which are high-dimensional and complex. Here, we introduce an improved method, Progeny Clustering, which is stability-based and exceptionally efficient in computing, to find the ideal number of clusters. The algorithm employs a novel Progeny Sampling method to reconstruct cluster identity, a co-occurrence probability matrix to assess the clustering stability, and a set of reference datasets to overcome inherent biases in the algorithm and data space. Our method was shown successful and robust when applied to two synthetic datasets (datasets of two-dimensions and ten-dimensions containing eight dimensions of pure noise), two standard biological datasets (the Iris dataset and Rat CNS dataset) and two biological datasets (a cell phenotype dataset and an acute myeloid leukemia (AML) reverse phase protein array (RPPA) dataset). Progeny Clustering outperformed some popular clustering evaluation methods in the ten-dimensional synthetic dataset as well as in the cell phenotype dataset, and it was the only method that successfully discovered clinically meaningful patient groupings in the AML RPPA dataset. PMID:26267476

  11. Anther culture properties of oat x wild red oat progenies and a search for RAPD markers associated with anther culture ability

    Directory of Open Access Journals (Sweden)

    E. KIVIHARJU

    2008-12-01

    Full Text Available A study was carried out to improve anther culture ability of the non-responsive cultivated oat, Avena sativa L. cv. Puhti by introgressing favourable alleles from the responsive wild red oat, Avena sterilis L. acc. CAV 2648. Anther culture ability of these parental lines and F2 progenies of their cross and two backcrosses was tested. Genotype effects were significant on all anther culture traits measured. The number of anther culture derived embryo-like structures was highest in acc. CAV 2648, and the number of green regenerants from the Puhti × CAV 2648 progeny. Anther culture response was greatly reduced in backcross progeny and was least in cv. Puhti. Random amplified polymorphic DNA (RAPD was used to test for marker associations with oat anther culture traits in a population of 38 F2 progenies. Two RAPD markers were putatively associated with improved production of green regenerants (one derived from acc. CAV 2648 and the other from cv. Puhti. One marker putatively associated with decreased albino plant regeneration (derived from acc. CAV 2648. These markers might be useful for selecting alleles for better anther culture ability among progeny of planned crosses. In addition, three markers, derived from acc. CAV 2648, were putatively associated with decreased anther culture response rates.;

  12. Genetic trends in the expected progeny difference of the asymptotic weight of Nelore females

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    Analía del Valle Garnero

    2006-01-01

    Full Text Available There are few studies on weight covering the full life cycle of Zebu cattle, and there is no entire growth description or mean growth pattern for animals belonging to this breed. In order to provide such data, 1,158 Nelore females born between 1985 and 1995 were weighed 14,563 times from birth to full growth maturity, in ten herds spread over seven Brazilian states. The Von Bertalanffy, Brody, logistic and Gompertz non-linear models were used to obtain the asymptotic weights (A and the maturation rates (K. The (covariance and breeding value components for A and K were obtained by using the multiple trait derivative free restricted maximum likelihood method under the animal model. Genetic trends were calculated in function of the mean expected progeny differences (EPD for the trait (A or K divided by the number of animals according to their year of birth. The genetic trends of the expected progeny difference with reference to the date of birth of the cows were, on average, -6.5g y-1 for A and 2.0g y-1 for K, close to zero as confirmed by the low (0.0023 to 0.003 coefficient of regression values. The curve parameters are recommended as a selection criterion to reach precocity and avoid adult weight increase in the female herd.

  13. Performance and genetic variation of big-leaf mahogany (Swietenia macrophylla King) in provenance and progeny trials in the Yucatan Peninsula of Mexico.

    Science.gov (United States)

    Kevyn E. Wightman; Sheila E. Ward; Jeremy P. Haggar; Bartolo Rodriguez Santiago; Jonathan P. Cornelius

    2008-01-01

    Stocks of the valuable big-leaf mahogany (Swietenia macrophylla King) are declining, and trials for growth and pest resistance are needed to select material for plantations. Seeds were collected from 67 open-pollinated trees from five provenances in the Yucatan Peninsula of Mexico and planted in three provenance/progeny trials in the state of Quintana Roo, Mexico, in...

  14. Sentinel Lymph Node Dissection to Select Clinically Node-negative Prostate Cancer Patients for Pelvic Radiation Therapy: Effect on Biochemical Recurrence and Systemic Progression

    Energy Technology Data Exchange (ETDEWEB)

    Grivas, Nikolaos, E-mail: n.grivas@nki.nl [Department of Urology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam (Netherlands); Wit, Esther [Department of Urology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam (Netherlands); Pos, Floris [Department of Radiation Oncology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam (Netherlands); Jong, Jeroen de [Department of Pathology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam (Netherlands); Vegt, Erik [Department of Nuclear Medicine, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam (Netherlands); Bex, Axel; Hendricksen, Kees; Horenblas, Simon [Department of Urology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam (Netherlands); KleinJan, Gijs [Department of Urology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam (Netherlands); Interventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical Center, Leiden (Netherlands); Rhijn, Bas van; Poel, Henk van der [Department of Urology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam (Netherlands)

    2017-02-01

    Purpose: To assess the efficacy of robotic-assisted laparoscopic sentinel lymph node (SLN) dissection (SLND) to select those patients with prostate cancer (PCa) who would benefit from additional pelvic external beam radiation therapy and long-term androgen deprivation therapy (ADT). Methods and Materials: Radioisotope-guided SLND was performed in 224 clinically node-negative patients scheduled to undergo external beam radiation therapy. Patients with histologically positive SLNs (pN1) were also offered radiation therapy to the pelvic lymph nodes, combined with 3 years of ADT. Biochemical recurrence (BCR), overall survival, and metastasis-free (including pelvic and nonregional lymph nodes) survival (MFS) rates were retrospectively calculated. The Briganti and Kattan nomogram predictions were compared with the observed pN status and BCR. Results: The median prostate-specific antigen (PSA) value was 15.4 ng/mL (interquartile range [IQR] 8-29). A total number of 834 SLNs (median 3 per patient; IQR 2-5) were removed. Nodal metastases were diagnosed in 42% of the patients, with 150 SLNs affected (median 1; IQR 1-2). The 5-year BCR-free and MFS rates for pN0 patients were 67.9% and 87.8%, respectively. The corresponding values for pN1 patients were 43% and 66.6%. The PSA level and number of removed SLNs were independent predictors of BCR and MFS, and pN status was an additional independent predictor of BCR. The 5-year overall survival rate was 97.6% and correlated only with pN status. The predictive accuracy of the Briganti nomogram was 0.665. Patients in the higher quartiles of Kattan nomogram prediction of BCR had better than expected outcomes. The complication rate from SLND was 8.9%. Conclusions: For radioisotope-guided SLND, the high staging accuracy is accompanied by low morbidity. The better than expected outcomes observed in the lower quartiles of BCR prediction suggest a role for SLN biopsy as a potential selection tool for the addition of pelvic radiation

  15. Divergência genética entre progênies de café robusta Genetic divergence among robusta coffe progenies

    Directory of Open Access Journals (Sweden)

    Milana Gonçalves Ivoglo

    2008-12-01

    recurrent selection and production of hybrids. The importance of the traits for divergence was, also, evaluated by the sequential discards the variables, according its relative contributions. The trial was carry out in an experimental field located in the Pólo Regional do Nordeste Paulista, Mococa-SP, using randomized blocks with 21 treatments and 24 replicates. The datas were analyzed by Tocher and UPGMA methods. The matrix of genetic divergence was gotten on the generalized Mahalanobis distance that it served of base for the formation of the groups. The employed methods had been efficient in detecting ample genetic variability between the evaluated progenies. Some groups of similarity had been identified. The IAC 2262, IAC 2290, IAC 2286, IAC 2292 and IAC 2291 progenies are indicated to compose programs of intercrosses, being considered the most promising in the attainment of segregantes populations or hybrid heterotics. The characteristics that had less contributed for the genetic divergence had been: canopy diameter before pruning, plant height before pruning and leaf area.

  16. Does circumcision influence recurrences in herpes genitalis?

    Directory of Open Access Journals (Sweden)

    Jerath V

    2009-01-01

    Full Text Available Background and Aims: The effect of therapeutic circumcision in men for preventing recurrences of herpes genitalis remains largely unelucidated despite its well documented albeit conflicting role in reducing the risk of acquiring sexually transmitted infections, including genital herpes. Methods: Twenty volunteer adult males with a history of recurrent herpes genitalis were included in the study after informed consent and circumcision was carried out. Twenty more adult males having recurrent herpes genitalis and registered in the clinic during the same period were selected as controls. All patients and controls were followed-up for recurrences of herpes genitalis. Results and Conclusions: Six patients and six controls did not follow-up. Seven patients reported no recurrences during 3-18 years, seven patients had two to six recurrences during 11-27 years of postcircumcision follow-up, 0.0080 (average recurrences per person per year as compared with 0.20 (average recurrences per person per year recorded before the circumcision. Two patients had first recurrence 11 years after the circumcision. In comparison, 14 controls had 0.17 (average recurrences per person per year, comparable with the number of recurrences in uncircumcised patients, and frequently at shorter intervals. Despite being a small study, the circumcision appears to reduce the number of recurrences on an average and evidently prolongs the disease-free period in between two recurrences.

  17. Variação genética, herdabilidades e ganhos na seleção para caracteres de crescimento em teste de progênies de Pinus caribaea var. bahamensis aos 20 anos de idade em Assis−SP. Genetic variation, herdabilities and gains in selection for growth traits in progeny test of Pinus caribaea var. bahamensis at 20 years of age in Assis−SP.

    Directory of Open Access Journals (Sweden)

    José Carlos Molina MAX

    2008-12-01

    Full Text Available O objetivo deste estudo foi estimarparâmetros genéticos para caracteres decrescimento em um teste de progênies de Pinuscaribaea var. bahamensis, aos 20 anos de idade.O teste de progênies foi instalado com sementes depolinização aberta provenientes de 89 árvoresmatrizes de Pinus caribaea var. bahamensis doCentro de Conservação Genética e Melhoramentode Pinheiros Tropicais - CCGMPT. Três progêniestestemunhas da mesma espécie e oito progênies deP. caribaea var. hondurensis, selecionadas emAssis para produção de resina, também foramincluídas no ensaio. O delineamento experimentalutilizado foi o látice triplo, com parcelas de dezplantas. O ensaio foi mensurado aos 20 anos deidade para diâmetro à altura do peito - DAP, alturatotal e volume real. Foram detectadas diferençassignificativas entre progênies para todos oscaracteres. A média dos caracteres para as 89progênies foi menor que a média calculada paraas testemunhas. As correlações genéticas foramaltas entre todos os caracteres (mínimog(DAPxaltura r = 0,71, P < 0,01. Foram detectados altoscoeficientes de variação genética e herdabilidadespara todos os caracteres estudados, o que mostra apossibilidade de se obter altos ganhos com a seleçãomassal e entre e dentro de progênies. Os ganhosesperados para plantios com 20 anos de idade,realizados em locais com as mesmas característicasambientais de Assis e com sementes coletadasapós a seleção no teste de progênies, foramestimados em 10,6% para DAP, 4,1% para altura,e 23,5% para volume.The aim of this study was to estimategenetic parameters for growth traits in a progenytest of Pinus caribaea var. bahamensis, at 20 yearsold. The trial was implanted using open-pollinatedseeds from 89 seed-trees of the Centro deConservação Genética e Melhoramento dePinheiros Tropicais - CCGMPT, three progenies ofthe same species selected in Assis and eightprogenies of P. caribaea var. hondurensis, alsoselected for gum yield in Assis

  18. Event dependent sampling of recurrent events

    DEFF Research Database (Denmark)

    Kvist, Tine Kajsa; Andersen, Per Kragh; Angst, Jules

    2010-01-01

    The effect of event-dependent sampling of processes consisting of recurrent events is investigated when analyzing whether the risk of recurrence increases with event count. We study the situation where processes are selected for study if an event occurs in a certain selection interval. Motivation...

  19. Measurement of indoor radon, thoron and their progeny concentrations in the dwellings of district Hamirpur, Himachal Pradesh

    Energy Technology Data Exchange (ETDEWEB)

    Bajwa, B.J.S.; Singh, P.; Singh, S. [Guru Nanak Dev University (India); Sahoo, B.K. [Bhabha Atomic Research Center - BARC (India)

    2014-07-01

    In the present investigation indoor radon, thoron and their progeny concentrations have been measured in the wide range of dwellings from 12 different villages situated in the uranium mineralized zones of Hamirpur district, Himachal Pradesh, India by using LR-115 type-?? based Pin-hole Radon-Thoron discriminating Twin-Cup dosimeters, direct radon and thoron progeny sensors (DRPS/DTPS). As inhalation doses are predominantly due to daughter products of radon and thoron and not due to gases, it is important to measure the decay products directly for health risk assessments. In the study region different types of houses were selected randomly according to methodologies described by Radiological Physics and Advisory Division (RPAD), BARC, Mumbai. The indoor radon concentrations in these dwellings have been found vary from 22 to 573 Bq/m{sup 3} with average value of 113.48 Bq/m{sup 3} and for thoron vary from 10 to 739 Bq/m{sup 3} with average value of 116.17 Bq/m{sup 3}. The progeny concentrations of radon and thoron are found within the limits of 8 to 141 Bq/m{sup 3} and 0.53 to 15.26 Bq/m{sup 3} respectively, with average values 36.97 Bq/m{sup 3} and 3.19 Bq/m{sup 3} respectively. The radon, thoron and their progeny concentration variations and the corresponding inhalation dose received by the inhabitants in this region will also be discussed in the light of the recommendations given by the International Commission on Radiological Protection (ICRP). Document available in abstract form only. (authors)

  20. Room model with three modal distributions of attached 220Rn progeny and dose conversion factor.

    Science.gov (United States)

    Nikezić, D; Stevanović, N

    2007-01-01

    Jacobi parametric room model was extended to the three modal distribution of aerosols, and applied to (220)Rn progeny. The computer program was developed to calculate ratios of progeny activity concentrations in different modes to (220)Rn concentration. The ratios are relatively small and they are given as functions on ventilation rate. Dose conversion factor (DCF) for (220)Rn progeny was calculated as 4.5 mSv WLM(-1), which is smaller by over three times than that for (222)Rn progeny.

  1. Introgression of QTLs Controlling Spikelet Fertility Maintains Membrane Integrity and Grain Yield in Improved White Ponni Derived Progenies Exposed to Heat Stress

    Directory of Open Access Journals (Sweden)

    P. Vivitha

    2017-01-01

    Full Text Available To increase the thermotolerance of improved White Ponni (IWP, two quantitative trait loci (QTLs, qHTSF1.1 and qHTSF4.1, controlling spikelet fertility under high-temperature stress, were introgressed from Nagina 22 into IWP through marker-assisted breeding. The progenies were subjected to foreground selection of target QTLs using simple sequence repent markers RM431 and RM5757 linked to qHTSF1.1 and qHTSF4.1, respectively. At each generation, foreground selection with single target QTL or both QTLs was done together. The QTL-positive plants were forwarded to next generation by selfing. The F2:3 progenies were subjected to phenotypic analyses under high-temperature stress at the flowering stage. Chlorophyll stability index, malondialdehyde content, grain yield, and yield-related components of the F2:3 progenies were measured. The progenies IWP-295, IWP-277 and IWP-246 harboring both qHTSF1.1 and qHTSF4.1 showed higher fertility percentages under high-temperature stress at the flowering stage. These QTLs were responsible for maintaining membrane integrity and yield under elevated temperature conditions.

  2. Metodología para evaluar progenies F5 a partir de selecciones individuales F4 de fríjol voluble en el sistema de relevo con maíz F5 lines from individuals selections of voluble bean intercropped with maize

    Directory of Open Access Journals (Sweden)

    Roman V. Alberto

    1988-12-01

    Full Text Available En el Centro Regional de Investiga, ción, ICA "La Selva", situado en el municipio de Rionegro, Antioquia, Colombia, se
    sembraron ocho ensayos entre 1986 y 1987, con el fin de determinar una nueva metodología para evaluar progenies F5, de fríjol voluble (Phaseolus vulgaris L. en el sistema
    de relevo con maíz (Zea mays L.. Se encontró que el sistema de parcelas pequeñas (0,84 m2 sirve para evaluar y tamizar grandes cantidades de material en lo que respecta al rendimiento, el peso de 100 semillas, días a madurez fisiológica y días a floración, util izando cuatro replicaciones. Se obtiene así un ahorro del 77 ,76% del área sembrada, en comparación con la siembra en parcelas de seis sitios y tres replicaciones, disminuyendo de esta manera los costos de la investigación.A series of eigth experiments were carried out at "la Selva" ICA's Experiment Station, located at Rionegro, Antioquía,
    Colombia at 2.100 m.s.n.m. between 1986 and 1987. The objetive was to determine a new methodology for the evaluation of F5 bean (Phaseolus vulgaris l. lines in relay intercropped planted with maize (Zeamsys l. It was found, for advanced lines, that one hill-plots (0.84 m-2 were not useful for detecting significant differences, but for F5 materials it was found that 4 replicates allowed to detect differences. The aboye result for F5 lines will permit to save 77 .8% of the area normally used in such works, i.e. by employing 6 hill plots and 3 replicates.

  3. All eggs are not equal: the maternal environment affects progeny reproduction and developmental fate in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Simon C Harvey

    Full Text Available BACKGROUND: Maternal effects on progeny traits are common and these can profoundly alter progeny life history. Maternal effects can be adaptive, representing attempts to appropriately match offspring phenotype to the expected environment and are often mediated via trade-offs between progeny number and quality. Here we have investigated the effect of maternal food availability on progeny life history in the free-living nematode Caenorhabditis elegans. METHODOLOGY/PRINCIPAL FINDINGS: The maternal environment affects both reproductive traits and progeny development. Comparisons of the progeny of worms from high and low maternal food environments indicates that low maternal food availability reduces progeny reproduction in good environments, increases progeny reproduction in poor environments and decreases the likelihood that progeny will develop as dauer larvae. These analyses also indicate that the effects on progeny are not a simple consequence of changes in maternal body size, but are associated with an increase in the size of eggs produced by worms at low maternal food availabilities. CONCLUSIONS/SIGNIFICANCE: These results indicate that the maternal environment affects both progeny reproduction and development in C. elegans and therefore that all progeny are not equal. The observed effects are consistent with changes to egg provisioning, which are beneficial in harsh environments, and of changes to progeny development, which are beneficial in harsh environments and detrimental in benign environments. These changes in progeny life history suggest that mothers in poor quality environments may be producing larger eggs that are better suited to poor conditions.

  4. Segregation of a major gene influencing ovulation in progeny of Lacaune meat sheep

    Directory of Open Access Journals (Sweden)

    Amigues Yves

    2002-07-01

    Full Text Available Abstract Inheritance of the ovulation rate (OR in the Lacaune meat breed was studied through records from a small nucleus of 36 hyper-prolific ewes screened on farms on the basis of their natural litter size, and from progeny data of three selected Lacaune sires. These sires were chosen at the AI centre according to their breeding values estimated for the mean and the variability of their daughters' litter size. Non-carrier Lacaune dairy ewes were inseminated to produce 121 F1 daughters and 27 F1 sons. Twelve sons (four from each sire were used in turn to inseminate non-carrier Lacaune dairy ewes providing 260 BC progeny ewes. F1 and BC progeny were brought from private farms and gathered after weaning on an experimental farm where ovulation rates were recorded in the first and second breeding seasons. With an average of 6.5 records each, the mean OR of hyper-prolific ewes was very high (5.34, and 38.4% of records showed a rate of 6 or more. F1 data showed high repeatability of OR (r = 0.54 within ewe, with significant variability among ewes. High OR (≥ 4 were observed in each family. A segregation analysis provided a significant likelihood ratio and classified the three founders as heterozygous. BC ewes also displayed high repeatability of OR (r = 0.47 and the mean OR varied considerably between families (from 1.24 to 1.78. Seven of the 12 BC families presented high-ovulating ewes (at least one record ≥ 4 and segregation analysis yielded a highly significant likelihood ratio as compared to an empirical test distribution. The high variability of the mean ovulation rate shown by a small group of daughters of BC ewes inseminated by putative carrier F1 rams, and the very high ovulation rate observed for some of these ewe lambs, confirmed the segregation of a major gene with two co-dominant alleles borne by an autosome. The difference between homozygous non-carriers and heterozygous ewes was about one ovulation on the observed scale and 2

  5. Mass selection in maize composites by means of selection indices

    NARCIS (Netherlands)

    Wolff, de F.

    1972-01-01

    General considerations

    A selection index may be applied to selection based either upon the performance of single plants or upon the performance of progenies. In the latter case however, index selection should only result in a modest gain in selection efficiency, when

  6. Genetic divergence in cocoa progenies for backcrossing program to witches' broom disease resistance

    Directory of Open Access Journals (Sweden)

    Milton Macoto Yamada

    2010-02-01

    Full Text Available Backcrossing has been little used in cacao breeding, particularly due to the long time required to transfer genes and recover the genetic background of the recurrent parent. The objective of this study was to select individuals, resulting from the backcross CEPEC-42 x SIC-19, genetically related to the recurrent parent SIC-19 by using RAPD molecular markers, among those with resistance to witches' broom. Of the 31 plants that clustered with SIC-19, 18 from the replanted material remained free of the disease in the field, with good vegetative aspect and, therefore can be used for backcross to reach the desired objective.

  7. Radon progeny deposition in track-detection diffusion chambers

    CERN Document Server

    Pressyanov, D; Simeonov, G

    1999-01-01

    The sensitivity dependence for cylindrical diffusion chambers that are used for radon track-detection measurements on the deposition fraction of radon progeny atoms has been theoretically studied (sensitivity is the ratio: area track density/integrated sup 2 sup 2 sup 2 Rn activity concentration). Experimentally, the sensitivity values of both the metal- and plastic-made chambers were determined. Results indicate that the experimental sensitivity for metal chambers is in accordance with the theoretical model while a deviation of 15% is observed for plastic chambers. The uncertainty in the sensitivity values that is related to possible variations of the diffusion coefficient for sup 2 sup 1 sup 8 Po atoms was estimated to be less than 10%. (author)

  8. First detection of radon progeny recoil tracks by MIMAC

    Science.gov (United States)

    Riffard, Q.; Santos, D.; Guillaudin, O.; Bosson, G.; Bourrion, O.; Bouvier, J.; Descombes, T.; Fourel, C.; Muraz, J.-F.; Lebreton, L.; Maire, D.; Colas, P.; Ferrer-Ribas, E.; Giomataris, I.; Busto, J.; Fouchez, D.; Brunner, J.; Tao, C.

    2017-06-01

    The MIMAC experiment is a μ-TPC project for directional dark matter search. Directional detection strategy is based on the measurement of the WIMP flux anisotropy due to the solar system motion with respect to the dark matter halo. The main purpose of MIMAC project is the measurement of nuclear recoil energy and 3D direction from the WIMP elastic scattering on target nuclei. Since June 2012 a bi-chamber prototype is operating at the Modane underground laboratory. In this paper, we report the first ionization energy and 3D track observations of NRs produced by the radon progeny. This measurement shows the capability of the MIMAC detector and opens the possibility to explore the low energy recoil directionality signature.

  9. RADON PROGENY AS AN EXPERIMENTAL TOOL FOR DOSIMETRY OF NANOAEROSOLS

    Energy Technology Data Exchange (ETDEWEB)

    Ruzer, Lev; Ruzer, Lev S.; Apte, Michael G.

    2008-02-25

    The study of aerosol exposure and dosimetry measurements and related quantitation of health effects are important to the understanding of the consequences of air pollution, and are discussed widely in the scientific literature. During the last 10 years the need to correlate aerosol exposure and biological effects has become especially important due to rapid development of a new, revolutionary industry ?-- nanotechnology. Nanoproduct commerce is predicted to top $1 trillion by 2015. Quantitative assessment of aerosol particle behavior in air and in lung deposition, and dosimetry in different parts of the lung, particularly for nanoaerosols, remains poor despite several decades of study. Direct measurements on humans are still needed in order to validate the hollow cast, animal studies, and lung deposition modeling. We discuss here the use of nanoscale radon decay products as an experimental tool in the study of local deposition and lung dosimetry for nanoaerosols. The issue of the safe use of radon progeny in such measurements is discussed based on a comparison of measured exposure in 3 settings: general population, miners, and in a human experiment conducted at the Paul Scherer Institute (PSI) in Switzerland. One of the properties of radon progeny is that they consist partly of 1 nm radioactive particles called unattached activity; having extremely small size and high diffusion coefficients, these particles can be potentially useful as radioactive tracers in the study of nanometer-sized aerosols. We present a theoretical and experimental study of the correlation between the unattached activity and aerosol particle surface area, together with a description of its calibration and method for measurement of the unattached fraction.

  10. Genetic similarity and relationships of DNA fingerprints with performance and with heterosis in Japanese quail lines from two origins and under reciprocal recurrent or within-line selection for early egg production

    OpenAIRE

    Minvielle, Francis; Coville, Jean-Luc; Krupa, Andrew; Monvoisin, Jean-Louis; Maeda, Yoshizane; Okamoto, Shin

    2000-01-01

    International audience; DNA fingerprints of Japanese quail male and female pure line breeders were obtained with probes 33.6, 33.15, and R18.1 and they yielded a total of 59 scoreable bands. Bandsharing ($0 < {\\rm BS} < 1$) was calculated within and between six quail lines of two origins, and under reciprocal recurrent (AA and BB), within-line (DD and EE) or no (PP and FF) selection. Twenty one pair types were compared. BS was 0.30 higher within line than between lines. BS with the control li...

  11. An Alpha spectrometer for measuring radon daughter individual activity concentration; Spettrometro Alfa per la misura delle concentrazioni individuali in attivita' della progenie del radon

    Energy Technology Data Exchange (ETDEWEB)

    Berico, M.; Formignani, M. [ENEA, Div. Protezione dell' Uomo e degli Ecosistemi, Centro Ricerche E. Clementel, Bologna (Italy); Mariotti, F. [Bologna Univ., Bologna (Italy). Dipt. di Fisica

    2001-07-01

    In the frame of the program of the Institute for Radiation Protection of ENEA, related to the evaluation of dose from radon and thoron progeny, an alpha spectrometer for the continuous air monitoring (CAM type) of radon and thoron has been realized. The constructive characteristics of the device are here presented together with energy and efficiency calibration. The device allows, by means of a screen type diffusion battery and a filter, to determinate the single radioactivity of each radionuclide of the progeny selecting them in relation to their diffusive behaviour (dichotomous particle size selection). The three-count filter method has been employed to measure the concentrations of {sup 218}Po, {sup 214}Pb and {sup 214}Bi in air. Radon and thoron effective doses using a dosimetric, instead of an epidemiologic approach, will be then evaluated. [Italian] Presso l'Istituto per la Radioprotezione, nell'ambito del programma di valutazione di dose da radon e' stato progettato e realizzato uno spettrometro alfa per il monitoraggio continuo in aria (CAM) della progenie del radon e del toron. Le caratteristiche costruttive dello strumento permettono, tramite l'utilizzo di batterie a diffusione a reti, di determinare l'attivita' individuale della progenie per diverse dimensioni granulometriche in particolare per la frazione attaccata e non al particolato amosferico con un taglio granulometrico di qualche nanometro. E' stato inoltre applicato un metodo spettrometrico a tre conteggi per il calcolo delle concentrazioni individuali della progenie del radon, {sup 218}Po, {sup 214}Pb and {sup 214}Bi, effettuando un conteggio alfa di {sup 218}Po e due conteggi alfa di {sup 214}Po. Tale informazione consentira' una valutazione della dose di radon utilizzando il modello dosimetrico in alternativa a quello epidemiologico.

  12. Cellulose content of tuber cell walls of progeny from a cross ...

    African Journals Online (AJOL)

    Two transgenic potato lines, csr2-1 and csr4-8, containing two different antisense genes, csr2 and csr4, respectively, were crossed to investigate the possibility of achieving reduction in cellulose content in the tuber cell walls of the progeny. The progeny containing both transgenes (double csr2/csr4 transformant) exhibited ...

  13. The multidimensional cube recurrence

    NARCIS (Netherlands)

    Henriques, A.G.|info:eu-repo/dai/nl/314131132; Speyer, D.E.

    2010-01-01

    We introduce a recurrence which we term the multidimensional cube recurrence, generalizing the octahedron recurrence studied by Propp, Fomin and Zelevinsky, Speyer, and Fock and Goncharov and the three-dimensional cube recurrence studied by Fomin and Zelevinsky, and Carroll and Speyer. The states of

  14. Genomic selection prediction accuracy in a perennial crop: case study of oil palm (Elaeis guineensis Jacq.).

    Science.gov (United States)

    Cros, David; Denis, Marie; Sánchez, Leopoldo; Cochard, Benoit; Flori, Albert; Durand-Gasselin, Tristan; Nouy, Bruno; Omoré, Alphonse; Pomiès, Virginie; Riou, Virginie; Suryana, Edyana; Bouvet, Jean-Marc

    2015-03-01

    Genomic selection empirically appeared valuable for reciprocal recurrent selection in oil palm as it could account for family effects and Mendelian sampling terms, despite small populations and low marker density. Genomic selection (GS) can increase the genetic gain in plants. In perennial crops, this is expected mainly through shortened breeding cycles and increased selection intensity, which requires sufficient GS accuracy in selection candidates, despite often small training populations. Our objective was to obtain the first empirical estimate of GS accuracy in oil palm (Elaeis guineensis), the major world oil crop. We used two parental populations involved in conventional reciprocal recurrent selection (Deli and Group B) with 131 individuals each, genotyped with 265 SSR. We estimated within-population GS accuracies when predicting breeding values of non-progeny-tested individuals for eight yield traits. We used three methods to sample training sets and five statistical methods to estimate genomic breeding values. The results showed that GS could account for family effects and Mendelian sampling terms in Group B but only for family effects in Deli. Presumably, this difference between populations originated from their contrasting breeding history. The GS accuracy ranged from -0.41 to 0.94 and was positively correlated with the relationship between training and test sets. Training sets optimized with the so-called CDmean criterion gave the highest accuracies, ranging from 0.49 (pulp to fruit ratio in Group B) to 0.94 (fruit weight in Group B). The statistical methods did not affect the accuracy. Finally, Group B could be preselected for progeny tests by applying GS to key yield traits, therefore increasing the selection intensity. Our results should be valuable for breeding programs with small populations, long breeding cycles, or reduced effective size.

  15. Correlações entre caracteres agronômicos em dois ciclos de seleção recorrente em milho-pipoca Correlations among agronomic traits in two recurrent selection cycles in popcorn

    Directory of Open Access Journals (Sweden)

    Máskio Daros

    2004-10-01

    Full Text Available Com o propósito de monitorar o comportamento de blocos gênicos em diferentes gerações de seleção recorrente em milho pipoca, quantificaram-se as correlações fenotípica, genotípica e de ambiente entre caracteres agronômicos, em dois ciclos de seleção recorrente. O primeiro ciclo foi formado por famílias de irmãos completos e o segundo ciclo por famílias S1. Embora não significativa, em ambos os ciclos, houve correlação genotípica negativa entre capacidade de expansão e rendimento de grãos. Para o segundo ciclo, as maiores magnitudes de correlação genotípica positiva ocorreram entre rendimento de grãos, número de espigas sadias e estande, indicando a possibilidade de sucesso na seleção de uma ou outra, com base no rendimento de grãos. Embora não significativa e em baixa magnitude, houve acréscimo da correlação genotípica entre rendimento de grãos e capacidade de expansão do primeiro para o segundo ciclo, denotando aumento na concentração de alelos favoráveis na população.This work was carried out to estimate environment, genotypic and phenotypic correlations among traits in two intrapopulational recurrent selection cycles in popcorn. The first cycle was composed by full sib families and the second cycle by S1 families. Seventy-five families of full sib and two hundred and twenty two families S1 were evaluated in randomized complete block design with two replicates in two environments. Although not statistically significant, genotypic correlation between grain yield and popping expansion was negative in both selection cycles. For the second cycle of recurrent selection, the highest positive estimates of genotypic correlations were found between: grain yield and number of ears and grain yield and stand, indicating the perspective of the success for selection based on grain yield. Although in less magnitude there was an increase in genotypic correlation between the grain yield and popping expansion from the

  16. Recurrent Novae — A Review

    Directory of Open Access Journals (Sweden)

    K. Mukai

    2015-02-01

    Full Text Available In recent years, recurrent nova eruptions are often observed very intensely in wide range of wavelengths from radio to optical to X-rays. Here I present selected highlights from recent multi-wavelength observations. The enigma of T Pyx is at the heart of this paper. While our current understanding of CV and symbiotic star evolution can explain why certain subset of recurrent novae have high accretion rate, that of T Pyx must be greatly elevated compared to the evolutionary mean. At the same time, we have extensive data to be able to estimate how the nova envelope was ejected in T Pyx, and it turns to be a rather complex tale. One suspects that envelope ejection in recurrent and classical novae in general is more complicated than the textbook descriptions. At the end of the review, I will speculate that these two may be connected.

  17. Maternal Diet and Insulin-Like Signaling Control Intergenerational Plasticity of Progeny Size and Starvation Resistance.

    Directory of Open Access Journals (Sweden)

    Jonathan D Hibshman

    2016-10-01

    Full Text Available Maternal effects of environmental conditions produce intergenerational phenotypic plasticity. Adaptive value of these effects depends on appropriate anticipation of environmental conditions in the next generation, and mismatch between conditions may contribute to disease. However, regulation of intergenerational plasticity is poorly understood. Dietary restriction (DR delays aging but maternal effects have not been investigated. We demonstrate maternal effects of DR in the roundworm C. elegans. Worms cultured in DR produce fewer but larger progeny. Nutrient availability is assessed in late larvae and young adults, rather than affecting a set point in young larvae, and maternal age independently affects progeny size. Reduced signaling through the insulin-like receptor daf-2/InsR in the maternal soma causes constitutively large progeny, and its effector daf-16/FoxO is required for this effect. nhr-49/Hnf4, pha-4/FoxA, and skn-1/Nrf also regulate progeny-size plasticity. Genetic analysis suggests that insulin-like signaling controls progeny size in part through regulation of nhr-49/Hnf4, and that pha-4/FoxA and skn-1/Nrf function in parallel to insulin-like signaling and nhr-49/Hnf4. Furthermore, progeny of DR worms are buffered from adverse consequences of early-larval starvation, growing faster and producing more offspring than progeny of worms fed ad libitum. These results suggest a fitness advantage when mothers and their progeny experience nutrient stress, compared to an environmental mismatch where only progeny are stressed. This work reveals maternal provisioning as an organismal response to DR, demonstrates potentially adaptive intergenerational phenotypic plasticity, and identifies conserved pathways mediating these effects.

  18. Inference of genetic diversity in popcorn S3 progenies.

    Science.gov (United States)

    Pena, G F; do Amaral, A T; Ribeiro, R M; Ramos, H C C; Boechat, M S B; Santos, J S; Mafra, G S; Kamphorst, S H; de Lima, V J; Vivas, M; de Souza Filho, G A

    2016-05-09

    Molecular markers are a useful tool for identification of complementary heterotic groups in breeding programs aimed at the production of superior hybrids, particularly for crops such as popcorn in which heterotic groups are not well-defined. The objective of the present study was to analyze the genetic diversity of 47 genotypes of tropical popcorn to identify possible heterotic groups for the development of superior hybrids. Four genotypes of high genetic value were studied: hybrid IAC 125, strain P2, and varieties UENF 14 and BRS Angela. In addition, 43 endogamous S3 progenies obtained from variety UENF 14 were used. Twenty-five polymorphic SSR-EST markers were analyzed. A genetic distance matrix was obtained and the following molecular diversity parameters were estimated: number of alleles, number of effective alleles, polymorphism information content (PIC), observed and expected heterozygosities, Shannon diversity index, and coefficient of inbreeding. We found a moderate PIC and high diversity index, indicating that the studied population presents both good discriminatory ability and high informativeness for the utilized markers. The dendrogram built based on the dissimilarity matrix indicated six distinct groups. Our findings demonstrate the genetic diversity among the evaluated genotypes and provide evidence for heterotic groups in popcorn. Furthermore, the functional genetic diversity indicates that there are informative genetic markers for popcorn.

  19. Seleção para aumento de produtividade e qualidade de frutos em abobrinha 'Piramoita' comparando dois métodos de melhoramento Selection for fruit yield and quality in 'Piramoita' summer squash comparing two breeding methods

    Directory of Open Access Journals (Sweden)

    Antonio Ismael Inácio Cardoso

    2007-01-01

    Full Text Available Este trabalho teve por objetivo comparar dois métodos de melhoramento quanto à seleção visando ao aumento de produtividade e qualidade de frutos na cv. Piramoita. Foram realizados três ciclos de seleção recorrente, com seleção de progênies autofecundadas (S1 e recombinação das melhores progênies para obtenção das populações melhoradas no primeiro (SR-1, segundo (SR-2 e terceiro (SR-3 ciclos. No método do "Single Seed Descent" (SSD as plantas foram autofecundadas por cinco gerações até a obtenção e avaliação de progênies S5. As nove melhores progênies foram selecionadas obtendo-se progênies endogâmicas selecionadas (SSD-1 a SSD-9 e essas progênies também foram intercruzadas para se obter a população P-SSD. Todas as populações obtidas (SR-1, SR-2, SR-3, P-SSD, SSD-1 a SSD-9, juntamente com a população original (cv. Piramoita, totalizando 14 tratamentos, foram avaliadas em um experimento em blocos ao acaso, com seis repetições e cinco plantas por parcela. As características avaliadas foram: produção de frutos (número e massa total e comercial, massa média de fruto comercial e taxa de frutos comerciais. Foram obtidos aumentos crescentes de produção de frutos comerciais com os ciclos de seleção recorrente, sendo a população SR-3 superior à 'Piramoita' para número de frutos por planta. Foi obtida uma progênie por SSD melhor que a população inicial e tão produtiva quanto à população SR-3. Deste modo, o método SSD pode vir a ser ótima alternativa no melhoramento genético de abobrinha, sem a necessidade de avaliação e seleção de progênies a cada safra, apenas quando essas já estiverem praticamente homozigotas.The objective of this work was to compare two breeding methods to improve fruit yield and quality in 'Piramoita' summer squash. Three cycles of recurrent selection were made, with evaluation and selection of S1 progenies and recombination of plants from selected progenies to obtain

  20. Assessment of Risk for Recurrent Diverticulitis

    Science.gov (United States)

    Sallinen, Ville; Mali, Juha; Leppäniemi, Ari; Mentula, Panu

    2015-01-01

    Abstract Recurrence of acute diverticulitis is common, and—especially complicated recurrence—causes significant morbidity. To prevent recurrence, selected patients have been offered prophylactic sigmoid resection. However, as there is no tool to predict whose diverticulitis will recur and, in particular, who will have complicated recurrence, the indications for sigmoid resections have been variable. The objective of this study was to identify risk factors predicting recurrence of acute diverticulitis. This is a retrospective cohort study of patients presenting with computed tomography–confirmed acute diverticulitis and treated nonresectionally during 2006 to 2010. Risk factors for recurrence were identified using uni- and multivariate Cox regression. A total of 512 patients were included. History of diverticulitis was an independent risk factor predicting uncomplicated recurrence of diverticulitis (1–2 earlier diverticulitis HR 1.6, 3 or more—HR 3.2). History of diverticulitis (HR 3.3), abscess (HR 6.2), and corticosteroid medication (HR 16.1) were independent risk factors for complicated recurrence. Based on regression coefficients, risk scoring was created: 1 point for history of diverticulitis, 2 points for abscess, and 3 points for corticosteroid medication. The risk score was unable to predict uncomplicated recurrence (AUC 0.48), but was able to predict complicated recurrence (AUC 0.80). Patients were further divided into low-risk (0–2 points) and high-risk (>2 points) groups. Low-risk and high-risk groups had 3% and 43% 5-year complicated recurrence rates, respectively. Risk for complicated recurrence of acute diverticulitis can be assessed using risk scoring. The risk for uncomplicated recurrence increases along with increasing number of previous diverticulitis. PMID:25715253

  1. Production of wide hybrids and backcross progenies between Diplotaxis erucoides and crop brassicas.

    Science.gov (United States)

    Vyas, P; Prakash, S; Shivanna, K R

    1995-03-01

    Intergeneric hybrids were produced between D. erucoides (♀), a wild species, and four cultivated species of Brassica, B. campestris, B. juncea, B. napus and B. oleracea, through embryo rescue. The hybrid nature of these plants was confirmed through morphological and cytological studies. Backcross pollinations with the pollen of the respective cultivars yielded BC progenies in the hybrids D. erucoides x B. juncea and D. erucoides x B. napus but not in D. erucoides x B. campestris and D. erucoides x B. oleracea. The hybrid D. erucoides x B. campestris was also used as a bridge species and crossed with B. juncea to raise the hybrid and backcross progenies. F2 progenies were more amenable than f1 hybrids for raising backcross progenies. Although D. erucoides is considered to be a close relative of B. campestris and B. oleracea, incompatibility barriers of this species with different cultivars do not reflect this relationship.

  2. [Genetic polymorphism of clones and their seed progeny in the scotch pine clone plantation].

    Science.gov (United States)

    Korshikov, I I; Demkovich, A E

    2010-01-01

    Genetic variation at 12 allozyme loci (10 of them being polymorphic ones) has been studied in the archive-clone plantation of 23 Pinus sylvestris plus-trees and their seed progeny in the south-east of Ukraine. More than a half of clones had 4-8 heterozygous loci, whereas their seed progeny was marked by a lower variation than maternal trees. Seed progeny was obtained at a high outcrossing rate (t(m) = 95%). The clone progeny was characterized by a high percentage of abnormal allele segregation in megagametophytes. There was also a high frequency of significant deviation in distribution of seed embryo genotypes from the theoretically expected one according to the Hardy-Weinberg law.

  3. Molecular marker analysis of F1 progenies and their parents for ...

    African Journals Online (AJOL)

    Molecular marker analysis of F1 progenies and their parents for carotenoids inheritance in African cassava (Manihot esculenta Crantz). DN Njoku, VE Gracen, SK Offei, IK Asante, EY Danquah, CN Egesi, E Okogbenin ...

  4. Assessment of radon equilibrium factor from distribution parameters of simultaneous radon and radon progeny measurements.

    Science.gov (United States)

    Chen, Jing; Marro, Leonora

    2011-11-01

    In Canada, a radon and radon progeny survey was carried out in the 1970s in 19 cities. To the authors' knowledge, this is the only large survey of simultaneous radon and radon progeny measurements up to the present time. However, the survey was carried out for the purpose of establishing geographic variation of radon and radon progeny; therefore, radon equilibrium factors, F, were not assessed at that time. From the summary results of this large simultaneous radon and radon progeny survey, the characteristics of radon equilibrium factor were assessed. The average F factor assessed from this survey in 12,576 houses is 0.54. The current assessment may indicate that the typical F value of 0.4 recommended by the United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR), and the International Commission on Radiological Protection (ICRP) could lead to a downward bias in the estimation of radon doses to the lung.

  5. Campylobacter epidemiology from breeders to their progeny in Eastern Spain.

    Science.gov (United States)

    Ingresa-Capaccioni, S; Jiménez-Trigos, E; Marco-Jiménez, F; Catalá, P; Vega, S; Marin, C

    2016-03-01

    While horizontal transmission is a route clearly linked to the spread of Campylobacter at the farm level, few studies support the transmission of Campylobacter spp. from breeder flocks to their offspring. Thus, the present study was carried out to investigate the possibility of vertical transmission. Breeders were monitored from the time of housing day-old chicks, then throughout the laying period (0 to 60 wk) and throughout their progeny (broiler fattening, 1 to 42 d) until slaughter. All samples were analyzed according with official method ISO 10272:2006. Results revealed that on breeder farms, Campylobacter isolation started from wk 16 and reached its peak at wk 26, with 57.0% and 93.2% of positive birds, respectively. After this point, the rate of positive birds decreased slightly to 86.0% at 60 wk. However, in broiler production all day-old chicks were found negative for Campylobacter spp, and the bacteria was first isolated at d 14 of age (5.0%), with a significant increase in detection during the fattening period with 62% of Campylobacter positive animals at the end of the production cycle. Moreover, non-positive sample was determined from environmental sources. These results could be explained because Campylobacter may be in a low concentration or in a non-culturable form, as there were several studies that successfully detected Campylobacter DNA, but failed to culture. This form can survive in the environment and infect successive flocks; consequently, further studies are needed to develop more modern, practical, cost-effective and suitable techniques for routine diagnosis. © 2016 Poultry Science Association Inc.

  6. The hESC line Envy expresses high levels of GFP in all differentiated progeny.

    Science.gov (United States)

    Costa, Magdaline; Dottori, Mirella; Ng, Elizabeth; Hawes, Susan M; Sourris, Koula; Jamshidi, Pegah; Pera, Martin F; Elefanty, Andrew G; Stanley, Edouard G

    2005-04-01

    Human embryonic stem cells (hESCs) have been advanced as a potential source of cells for use in cell replacement therapies. The ability to identify hESCs and their differentiated progeny readily in transplantation experiments will facilitate the analysis of hESC potential and function in vivo. We have generated a hESC line designated 'Envy', in which robust levels of green fluorescent protein (GFP) are expressed in stem cells and all differentiated progeny.

  7. Control of respirable particles and radon progeny with portable air cleaners

    Energy Technology Data Exchange (ETDEWEB)

    Offermann, F.J.; Sextro, R.G.; Fisk, W.J.; Nazaroff, W.W.; Nero, A.V.; Revzan, K.L.; Yater, J.

    1984-02-01

    Eleven portable air cleaning devices have been evaluated for control of indoor concentrations of respirable particles and radon progeny. Following injection of cigarette smoke and radon in a room-size chamber, decay rates for particles and radon progeny concentrations were measured with and without air cleaner operation. Particle concentrations were obtained for total number concentration and for number concentration by particle size. In tests with no air cleaner the natural decay rate for cigarette smoke was observed to be 0.2 hr/sup -1/. Air cleaning rates for particles were found to be negligible for several small panel-filters, a residential ion-generator, and a pair of mixing fans. The electrostatic precipitators and extended surface filters tested had significant particle removal rates, and a HEPA-type filter was the most efficient air cleaner. The evaluation of radon progeny control produced similar results; the air cleaners which were effective in removing particles were also effective in removing radon progeny. At low particle concentrations plateout of the unattached radon progeny is an important removal mechanism. Based on data from these tests, the plateout rate for unattached progeny was found to be 15 hr/sup -1/. The unattached fraction and the overall removal rate due to deposition of attached and unattached nuclides have been estimated for each radon decay product as a function of particle concentration. While air cleaning can be effective in reducing total radon progeny, concentrations of unattached radon progeny can increase with increasing air cleaning. 39 references, 26 figures, 9 tables.

  8. Effects of exposing Drosophila melanogaster parents to ethanol on expression of vestigial in their progeny.

    Science.gov (United States)

    Giesel, J T; Niemann, M M

    1985-03-01

    When parental Drosophila melanogaster were chronically exposed at 28 degrees C or 24 degrees C to ethanol during their larval and pupal stages of development, their progeny, produced when parents were 5-16-day-old adults, showed modified expression of vestigial alleles in heterozygous and homozygous combinations. Parental alcohol effects were dependent on parental rearing temperature. We conclude that parental environment (alcohol, temperature) causes heritable but transitory changes in progeny phenotype that are elicited by exposure of germ cells to alcohol.

  9. Response to low-dose herbicide selection in self-pollinated Avena fatua.

    Science.gov (United States)

    Busi, Roberto; Girotto, Marcelo; Powles, Stephen B

    2016-03-01

    When applied at the correct plant stage and dose, herbicides are highly toxic to plants. At reduced, low herbicide doses (below the recommended dose) plants can survive and display continuous and quantitative variation in dose-survival responses. Recurrent (directional) selection studies can reveal whether such a phenotypic variation in plant survival response to low herbicide dose is heritable and leads to herbicide resistance. In a common experimental garden study, we have subjected a susceptible population of self-pollinated hexaploid Avena fatua to low-dose recurrent selection with the ACCase-inhibiting herbicide diclofop-methyl for three consecutive generations. Significant differences in response to low-dose diclofop-methyl selection were observed between the selected progenies and parent plants, with a twofold diclofop-methyl resistance and cross-resistance to ALS-inhibiting herbicides. Thus, the capacity of self-pollinated A. fatua to respond to low-dose herbicide selection is marginal, and it is much lower than in cross-pollinated L. rigidum. Lolium rigidum in the same experiment evolved 40-fold diclofop-methyl resistance by progressive enrichment of quantitative resistance-endowing traits. Cross-pollination rate, genetic variation and ploidy levels are identified as possible drivers affecting the contrasting capacity of Avena versus Lolium plants to respond to herbicide selection and the subsequent likelihood of resistance evolution at low herbicide dose usage. © 2015 Society of Chemical Industry.

  10. [Evaluation of Reproductive Health of Chickens and Their Progeny at a Chronic Effect of 131I].

    Science.gov (United States)

    Boudarkov, V A

    2015-01-01

    A reproductive health of hens exposed to 131I in a 30-day period with daily quantities ranging from 0.11 to 4.6 MBq/kg and 6 progenies of their offspring was evaluated. We determined that 131I did not change significantly the reproductive potential of hens if administered at a dose of 0.11 MBq, while it raised at 1.1 MBq, progressively decreased after a short-time increase at 2.1 MBq and was inhabited up to its irreversible extinction at 4.6 MBq. Irrespective of the isotope quantity administered, a decline occurred in the birth rate of the progeny where hens dominated in the sex composition. The reproductive potential (i.e., laying capacity) of the offspring of three chicken progenies that had been administered 131I at 0.11 MBq/kg, progenies 3 and 5 that had been administered 1.1 MBq/kg and progeny 1 affected with 2.1 MBq/kg increased, while for chicken progenies 1, 2, 4 and 6 that had been given 131I at 1.1. MBq/kg the reproductive capacity was within the normal range or decreased.

  11. Meiotic behavior in apomictic Brachiaria ruziziensis × B. brizantha (Poaceae progenies

    Directory of Open Access Journals (Sweden)

    Veridiana Aparecida Fuzinatto

    2012-12-01

    Full Text Available Hybrids combining desirable traits from divergent parents are the main objective of some Brachiaria (Syn. Urochloa P. Beauv. breeding programs. There is great interest in the development of apomictic hybrid cultivars that combine desirable genes such as resistance to spittlebugs, high nutritive value, and tolerance to acid soils. Microsporogenesis of six apomictic progenies resulting from a tetraploid (2n = 4x = 36 cross between B. ruziziensis × B. brizantha was evaluated under light microscopy. Genetic recombination, ensured by multivalent chromosome association and crossing-over at prophase occurred in low frequency among progenies, and in one, recombination was almost nonexistent. The percentage of meiocytes with meiotic abnormalities among progenies ranged from 16.6 % to 85.6 %. Besides an observed irregular chromosome segregation typical of polyploid hybrids in these five progenies, putative meiotic mutations characterized as desynapsis and divergent spindle organization occurred in three progenies. These anomalies caused frequent fractionation of the genome into several microspores of different sizes. In Brachiaria, new cultivars must be apomictic to fix the genotype. However, Brachiaria is a pseudogamous apomict, and viable gametes are necessary to produce viable seeds. Considering meiotic behavior, only two progenies are promising for advancement in the breeding program.

  12. Cytogenetic changes in the liver of progeny of irradiated male rats

    Energy Technology Data Exchange (ETDEWEB)

    Kropacova, K.; Slovinska, L.; Misurova, E. [P.J. Safarik Univ., Kosice (Slovakia)

    2002-06-01

    The transgenerational transmission of radiation damage of rat genom was studied on the basis of cytogenetic changes in somatic cells (hepatocytes). It was found, that the irradiation of rat males with dose of 3 Gy of gamma radiation caused latent cytogenetic damage to the liver, which was expressed during the course of an induced proliferation of hepatocytes (by partial hepatectomy) by lower proliferative activity and a high frequency of chromosomal aberrations. In the progeny of irradiated males (in the F{sub 1} generation), the radiation damage to DNA was manifested by similar changes, i.e. by lower proliferation activity and increase in ''spontaneous'' chromosomal aberration occurrence in liver regeneration after partial hepatectomy. Irradiating the progeny of irradiated males (the total radiation load of the progeny being 3 Gy+3 Gy) caused slighter changes in compared with irradiating the progeny of non-irradiated control males (the total radiation load of the progeny being 0 Gy+3 Gy), which suggests some kind of adaptive response, which was also found in other experimental systems and parameters. An analogous course of RNA and DNA quantitative changes in the liver of the F{sub 0} and F{sub 1} generations of rats confirms the partial transmission of radiation damage of genom to the progeny. (author)

  13. Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding

    Directory of Open Access Journals (Sweden)

    Lorena V. Armando

    2017-09-01

    Full Text Available Panicum coloratum var. makarikariense, a perennial grass native to Africa, is adapted to a wide range of soil and climatic conditions with potential to be used as forage in tropical and semi-arid regions around the world. Our objective was to understand how the pollination mode affects viable seed production and further survival of the progeny. We evaluated self- and open-pollinated progenies from different accessions by measuring the seed production of the parents and their germination performance, germination rate and seedling survival. Parents and progeny were also fingerprinted with Simple Sequence Repeats (SSR. Progeny produced through open-pollination resulted in significantly more filled seeds and superior seedling survival than self-pollination. These results indicate that accessions studied here rely heavily on cross-pollination, whereas the contribution of self-pollinated offspring to the population is likely to be low. SSR profiles showed that, on average, 85% of the progeny (arising from cross-pollination possessed paternal specific markers and 100% of them were genetically different from the maternal genotype. All plants examined had 4x = 36 chromosomes. Overall, our findings indicate that var. makarikariense is able to generate highly polymorphic progeny through segregation and recombination. This study provides reference information for the formulation of appropriate strategies for pasture germplasm management, conservation and development of breeding programs. 

  14. Virgin olive oil phenolic profile and variability in progenies from olive crosses.

    Science.gov (United States)

    El Riachy, Milad; Priego-Capote, Feliciano; León, Lorenzo; Luque de Castro, María Dolores; Rallo, Luis

    2012-09-01

    The progressive transformation of olive growing and the increasing demands for high-quality monovarietal virgin olive oil (VOO) have triggered interest in olive breeding programs, in which the evaluation of the new genotypes is the basis for obtaining new olive cultivars. In this work, the phenolic composition of VOOs from two progenies from crosses between 'Arbequina', 'Arbosana' and 'Sikitita' has been evaluated along two years. A higher degree of variation was observed in segregating population as compared to genitors. The results also showed that the variability within crosses constitutes the major contribution to total variance for all considered parameters (>92% of total sum of squares). All compounds under study were present in oils obtained in both years; however, clear differences in their concentrations were observed between years. Olive breeding can indeed provide genotypes that produce oils with improved phenolic profiles as compared to traditional cultivars. In addition, the data showed that selection as a function of tyrosol content could be achieved in only one crop year. Finally, p-coumaric acid was the unique component able to discriminate between both crop years under study. Copyright © 2012 Society of Chemical Industry.

  15. A study of indoor radon, thoron and their progeny measurement in Tosham region Haryana, India

    Directory of Open Access Journals (Sweden)

    Prabhjot Singh

    2015-04-01

    Full Text Available In the present study indoor radon, thoron and their decay products concentrations have been measured using the newly developed LR-115 type-ІІ based Radon-Thoron discriminating twin-cup dosimeters with single entry face, direct radon and thoron progeny sensors (DRPS/DTPS respectively. The annual annihilation dose has been assessed from measured radionuclide concentration to find out major contributor of lung cancer in the study area. The measurements have been carried out in NINETY dwellings of THIRTEEN different villages situated in and around the Tosham region. This region is known to be composed of acidic volcanic and associated granites. Dwellings were selected mainly targeting different type building material used in construction of houses like concrete–brick, mud-brick, and mud-thatches along with an idea of different ventilation conditions which affects the equilibrium factor (EF. The EF in this region has been varying from 0.20 to 0.72 and 0.03–0.13 for indoor radon and thoron respectively. The average inhalation dose observed in dwellings of different villages varies from 1.33 ± 0.31–3.36 ± 0.72 mSv/y that lies within the safe limits recommended by ICRP (2011.

  16. Differential diagnosis of recurrent bronchitis in children

    Directory of Open Access Journals (Sweden)

    I. I. Zakirov

    2016-01-01

    Full Text Available The interest of the doctors — pediatricians to recurrent bronchitis is caused by its high specific weight in the structure of bronchopulmonary pathology in children, the complexity and the need to search the differential selection of an individual regimen and prevention. Recurrent bronchitis with bronchoobstructive syndromes patient is regarded as a predictor of asthma, however, erroneously consider these nosological forms as the stages of a single pathophysiological chain as congenital and acquired diseases of respiratory system, pathology of gastro-intestinal tract, cardio-vascular system can masquerade as recurrent bronchitis. The aim of this work — to analyze the current medical literature on the problem of differential search causes leading recurrent respiratory syndrome in children. Materials and methods. The review publications domestic and foreign authors studied data from clinical and epidemiological studies. Results and its discussion. Anatomical physiological characteristics of the respiratory tract, complicated premorbid background, the re-infection of the child respiratory infection contribute to the recurrent course of bronchitis. The most common recurrent bronchitis is transient, recurrent bronchitis episodes completely stoped with the age of the child. Recurrent respiratory infections can fix bronchial hyperreactivity and with the presence of aggravated allergic history lead to the development of asthma. Severe bronchitis, resistance to standard scheme of therapy, the frequent recurrence of respiratory syndrome dictate the need to expand the diagnostic research to clarify the leading cause of recurrent bronchitis. Conclusion. Recurrent bronchitis is considered as a separate nosological form. At the same time, by repeated episodes of respiratory infection may be masked by malformations, hereditary diseases of respiratory system, bronchus foreign body microaspiration syndrome, pathology of the cardio-vascular system. 

  17. Inhalation dose due to radon, thoron, and progenies in dwellings of a hill station.

    Science.gov (United States)

    Sivakumar, R

    2017-02-01

    The general public spends a major portion of their time in an indoor environment and hence receives a considerable amount of radiation. Knowledge about indoor radiation is important in order to arrive at the actual effective dose received by residents. The indoor radon, thoron, and progeny concentrations observed in the present study were found to vary with seasons of a given year. The highest and lowest indoor average radon, thoron, and progeny levels were observed during winter and summer seasons, respectively. The concentrations of indoor radon, thoron, and progenies were found to vary with the type of houses. The highest (222)Rn, (220)Rn, and progeny concentrations were observed in mud houses and the lowest values were recorded in wooden houses. The indoor (222)Rn concentration correlated well with concentration of its grandparent (238)U in underlying soil with a correlation coefficient of 0.87. The correlation between indoor (220)Rn and (232)Th in the underlying soil was found to be 0.64. The estimated effective doses received by the general public in the present study due to indoor radon and thoron were 1.49 ± 0.49 and 1.30 ± 0.53 mSv/year, respectively. The annual effective doses due to radon and thoron progenies were estimated as 0.76 ± 0.27 and 0.47 ± 0.23 mSv/year, respectively. The contributions from (222)Rn, (220)Rn, and corresponding progenies to the annual effective doses received were 37, 32, 19, and 12%, respectively. The general public living in the study area receives an inhalation dose of 4.02 mSv/year due to indoor radon, thoron, and progenies, which were found to be less than the action limit of ICRP 2009.

  18. Chromosomal Behavior during Meiosis in the Progeny of Triticum timopheevii × Hexaploid Wild Oat

    Science.gov (United States)

    An, Hongzhou; Hu, Mei; Li, Pengfei; Geng, Guangdong; Zhang, Qingqin; Zhang, Suqin

    2015-01-01

    The meiotic behavior of pollen mother cells (PMCs) of the F2 and F3 progeny from Triticum timopheevii × hexaploid wild oat was investigated by cytological analysis and sequential C-banding-genomic in situ hybridization (GISH) in the present study. A cytological analysis showed that the chromosome numbers of the F2 and F3 progeny ranged from 28 to 41. A large number of univalents, lagging chromosomes, chromosome bridges and micronuclei were found at the metaphase I, anaphase I, anaphase II and tetrad stages in the F2 and F3 progeny. The averages of univalents were 3.50 and 2.73 per cell, and those of lagging chromosomes were 3.37 and 1.87 in the F2 and F3 progeny, respectively. The PMC meiotic indices of the F2 and F3 progeny were 12.22 and 20.34, respectively, indicating considerable genetic instability. A sequential C-banding-GISH analysis revealed that some chromosomes and fragments from the hexaploid wild oat were detected at metaphase I and anaphase I in the progeny, showing that the progeny were of true intergeneric hybrid origin. The alien chromosomes 6A, 7A, 3C and 2D were lost during transmission from F2 to F3. In addition, partial T. timopheevii chromosomes appeared in the form of univalents or lagging chromosomes, which might result from large genome differences between the parents, and the wild oat chromosome introgression interfered with the wheat homologues’ normally pairing. PMID:25950431

  19. Chromosomal Behavior during Meiosis in the Progeny of Triticum timopheevii × Hexaploid Wild Oat.

    Directory of Open Access Journals (Sweden)

    Hongzhou An

    Full Text Available The meiotic behavior of pollen mother cells (PMCs of the F2 and F3 progeny from Triticum timopheevii × hexaploid wild oat was investigated by cytological analysis and sequential C-banding-genomic in situ hybridization (GISH in the present study. A cytological analysis showed that the chromosome numbers of the F2 and F3 progeny ranged from 28 to 41. A large number of univalents, lagging chromosomes, chromosome bridges and micronuclei were found at the metaphase I, anaphase I, anaphase II and tetrad stages in the F2 and F3 progeny. The averages of univalents were 3.50 and 2.73 per cell, and those of lagging chromosomes were 3.37 and 1.87 in the F2 and F3 progeny, respectively. The PMC meiotic indices of the F2 and F3 progeny were 12.22 and 20.34, respectively, indicating considerable genetic instability. A sequential C-banding-GISH analysis revealed that some chromosomes and fragments from the hexaploid wild oat were detected at metaphase I and anaphase I in the progeny, showing that the progeny were of true intergeneric hybrid origin. The alien chromosomes 6A, 7A, 3C and 2D were lost during transmission from F2 to F3. In addition, partial T. timopheevii chromosomes appeared in the form of univalents or lagging chromosomes, which might result from large genome differences between the parents, and the wild oat chromosome introgression interfered with the wheat homologues' normally pairing.

  20. Acute recurrent polyhydramnios

    DEFF Research Database (Denmark)

    Rode, Line; Bundgaard, Anne; Skibsted, Lillian

    2007-01-01

    Acute recurrent polyhydramnios is a rare occurrence characterized by a poor fetal outcome. This is a case report describing a 34-year-old woman presenting with acute recurrent polyhydramnios. Treatment with non-steroidal anti-inflammatory drugs (NSAID) and therapeutic amniocenteses was initiated...... an enzyme-linked immunosorbent assay. As in normal pregnancies, amniotic prolactin levels decreased by 80% from highest to lowest value in this case of resolving acute recurrent polyhydramnios....

  1. Recurrent fever in children

    OpenAIRE

    Sofia Torreggiani; Giovanni Filocamo; Susanna Esposito

    2016-01-01

    Children presenting with recurrent fever may represent a diagnostic challenge. After excluding the most common etiologies, which include the consecutive occurrence of independent uncomplicated infections, a wide range of possible causes are considered. This article summarizes infectious and noninfectious causes of recurrent fever in pediatric patients. We highlight that, when investigating recurrent fever, it is important to consider age at onset, family history, duration of febrile episodes,...

  2. Measurement of the deposited activity of the short-lived radon progeny in the human respiratory tract

    Energy Technology Data Exchange (ETDEWEB)

    Vezzu, G.; Butterweck-Dempewolf, G.; Schuler, C. [Paul Scherrer Inst., Villigen (Switzerland). Div. for Radiation Protection and Waste Management

    1998-12-31

    Volunteers were exposed in the radon chamber at Paul Scherrer Institut to an atmosphere enriched with highly unattached radon progeny. The deposited radon progeny activity in the respiratory tract of the volunteers was determined using a low level in-vivo counter. The detector arrangement and its calibration for the measurement of deposited radon progeny activity is described and the results for a mouth and a nose breathing volunteer are presented. For the nose breathing volunteer 55% of the deposited radon progeny activity was located in the head and the remaining 45% in the chest whereas for the mouth breathing volunteer 25% was located in the head and the remaining 75% in the chest. A mean clearance half-life for the deposited radon progeny from the respiratory tract of (2{+-}1) h was obtained from the analyses of the temporal behaviour of the deposited radon progeny activity in the head. (orig.)

  3. Glucosinolates in Self-crossed Progenies of Monosomic Cabbage Alien Addition Lines in Chinese Cabbage

    Directory of Open Access Journals (Sweden)

    Gu Aixia

    2015-09-01

    Full Text Available Brassica species have been reported to possess cancer preventive activity due to glucosinolates (GLS and their derived properties. Many studies on GLS have focused on Brassica oleracea and Brassica rapa. However, information on GLS in progeny between Chinese cabbage (B. rapa ssp. pekinensis and cabbage (B. oleracea var. capitata remains limited. In this study, eight GLS were detected in the self-crossed progenies of monosomic cabbage alien addition lines in Chinese cabbage (Chinese cabbage – cabbage MAALs and parental Chinese cabbage, and nine GLS were detected in the parental cabbage. The variation of GLS content ranges was greater in the progeny than in the parental Chinese cabbage. The nine GLS identified were subjected to PCA to evaluate the differences among progeny and parents. Eight progeny samples had a comprehensive principal component score closer to or greater than that of cabbage, and four of them exhibited glucoraphanin (GRA and total GLS contents greater than that of Chinese cabbage with the relative content of total indolic GLS was greater than 50%. These results offered new opportunity to improve GLS-containing of Chinese cabbage using genes from cabbage.

  4. Observation of patients with vesicoureteral reflux off antibiotic prophylaxis: physician bias on patient selection and risk factors for recurrent febrile urinary tract infection.

    Science.gov (United States)

    Drzewiecki, Beth A; Thomas, John C; Pope, John C; Adams, Mark C; Brock, John W; Tanaka, Stacy T

    2012-10-01

    Observation off continuous antibiotic prophylaxis is an option for vesicoureteral reflux. We evaluated the characteristics of patients observed off continuous antibiotic prophylaxis and risk factors for febrile urinary tract infection. We identified children 1 to 18 years old with primary vesicoureteral reflux between January 1, 2010 and December 31, 2010. We excluded patients with prior surgical correction from analysis. We recorded age, gender, race/ethnicity, primary language, insurance carrier, age at vesicoureteral reflux diagnosis, initial presentation and vesicoureteral reflux severity. We quantified bladder and bowel dysfunction with a validated questionnaire if toilet trained. We compared patients off vs on continuous antibiotic prophylaxis with the chi-square test for categorical variables and the Mann-Whitney U test for continuous variables. We used a univariate Cox proportional hazards model to assess predictors of febrile urinary tract infection during observation off continuous antibiotic prophylaxis. Of 529 eligible patients 224 were observed off continuous antibiotic prophylaxis. Patients off continuous antibiotic prophylaxis tended to be older (p <0.001), to be older at diagnosis (p <0.001), to have an initial presentation other than febrile urinary tract infection (p = 0.05), to have nondilating vesicoureteral reflux on most recent cystogram (p <0.001) and to have lower bladder/bowel dysfunction scores if toilet trained (p <0.001). Of the patients off continuous antibiotic prophylaxis a febrile urinary tract infection developed in 19 (8.5%). Risk factors associated with febrile urinary tract infection included initial presentation of multiple febrile urinary tract infections (p = 0.03), older age at diagnosis (p = 0.03) and older age starting observation off continuous antibiotic prophylaxis (p = 0.0003). Criteria to select patients with vesicoureteral reflux for observation off continuous antibiotic prophylaxis remain poorly defined in the

  5. Role of salvage stereotactic body radiation therapy in post-surgical loco-regional recurrence in a selected population of non-small cell lung cancer patients.

    Science.gov (United States)

    Agolli, Linda; Valeriani, Maurizio; Carnevale, Alessia; Falco, Teresa; Bracci, Stefano; De Sanctis, Vitaliana; Minniti, Giuseppe; Enrici, Riccardo Maurizi; Osti, Mattia Falchetto

    2015-03-01

    This is a retrospective analysis of a selected series of high-risk non-small cell lung cancer (NSCLC) patients with post-surgical loco-regional relapse treated with salvage stereotactic body radiotherapy (SBRT). Outcome and toxicity profiles were assessed. Twenty-eight patients (unfit for surgery or systemic therapy) with 30 lesions underwent salvage SBRT as an alternative therapy because of advanced age, co-morbid conditions or no response obtained from other treatments. Complete and partial responses were 16% and 70%, respectively. Local progression was observed in 3 patients. Regional relapse occurred in 5 patients. Distant progression occurred in 10 patients. The 2-year overall survival (OS) and disease-free survival (DFS) were 57.5% and 36.6%, respectively. Radiation acute pneumonitis occurred as follows: three patients developed grade 1, two patients experienced grade 2 and one patient experienced grade 3 toxicity. Stereotactic body radiotherapy could have an alternative role in isolated loco-regional relapse in patients unfit or resistant to other therapies. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  6. The selective treatment of clinical mastitis based on on-farm culture results: II. Effects on lactation performance, including clinical mastitis recurrence, somatic cell count, milk production, and cow survival.

    Science.gov (United States)

    Lago, A; Godden, S M; Bey, R; Ruegg, P L; Leslie, K

    2011-09-01

    The objective of this multi-state, multi-herd clinical trial was to report on the efficacy of using an on-farm culture system to guide strategic treatment decisions in cows with clinical mastitis. The study was conducted in 8 commercial dairy farms ranging in size from 144 to 1,795 cows from Minnesota, Wisconsin, and Ontario, Canada. A total of 422 cows affected with mild or moderate clinical mastitis in 449 quarters were randomly assigned to either (1) a positive-control treatment program or (2) an on-farm culture-based treatment program. Quarter cases assigned to the positive-control group received immediate on-label intramammary treatment with cephapirin sodium. Quarters assigned to the culture-based treatment program were not treated until the results of on-farm culture were determined after 18 to 24h of incubation. Quarters in the culture-based treatment program that had gram-positive growth or a mixed infection were treated according to label instruction using intramammary cephapirin sodium. Quarters assigned to the culture-based treatment program that had gram-negative or no-growth did not receive intramammary therapy. It was already reported in a companion paper that the selective treatment of clinical mastitis based on on-farm culture results decreases antibiotic use by half and tends to decrease milk withholding time without affecting short-term clinical and bacteriological outcomes. The present article reports on long-term outcomes of the aforementioned study. No statistically significant differences existed between cases assigned to the positive-control program and cases assigned to the culture-based treatment program in risk and days for recurrence of clinical mastitis in the same quarter (35% and 78 d vs. 43% and 82 d), linear somatic cell count (4.2 vs. 4.4), daily milk production (30.0 vs. 30.7 kg), and risk and days for culling or death events (28% and 160 d vs. 32% and 137 d) for the rest of the lactation after enrollment of the clinical mastitis

  7. Behavior of {sup 222}Rn and its progeny in high-rise buildings

    Energy Technology Data Exchange (ETDEWEB)

    Leung, J.K.C.; Tso, M.Y.W.; Ho, C.W. [Univ. of Hong Kong (Hong Kong). Radioisotope Unit

    1998-09-01

    Unlike detached or semi-detached houses, the main source of indoor radon in high-rise buildings is the building material. Radon released from the building material will be removed by ventilation, either forced or natural, so that its concentration, its progeny`s concentration, and the equilibrium between the two will be different for different types of buildings and environmental factors. A number of surveys were carried out in buildings throughout the territory to look at the seasonal variation of indoor {sup 222}Rn levels; the dependence of indoor {sup 222}Rn concentration on the living environment; the indoor gamma dose rate and its relation to indoor {sup 222}Rn concentration; and the dependence of {sup 222}Rn progeny concentration and equilibrium factor on the environment in high-rise buildings.

  8. Transcriptomic Analysis Using Olive Varieties and Breeding Progenies Identifies Candidate Genes Involved in Plant Architecture.

    Science.gov (United States)

    González-Plaza, Juan J; Ortiz-Martín, Inmaculada; Muñoz-Mérida, Antonio; García-López, Carmen; Sánchez-Sevilla, José F; Luque, Francisco; Trelles, Oswaldo; Bejarano, Eduardo R; De La Rosa, Raúl; Valpuesta, Victoriano; Beuzón, Carmen R

    2016-01-01

    Plant architecture is a critical trait in fruit crops that can significantly influence yield, pruning, planting density and harvesting. Little is known about how plant architecture is genetically determined in olive, were most of the existing varieties are traditional with an architecture poorly suited for modern growing and harvesting systems. In the present study, we have carried out microarray analysis of meristematic tissue to compare expression profiles of olive varieties displaying differences in architecture, as well as seedlings from their cross pooled on the basis of their sharing architecture-related phenotypes. The microarray used, previously developed by our group has already been applied to identify candidates genes involved in regulating juvenile to adult transition in the shoot apex of seedlings. Varieties with distinct architecture phenotypes and individuals from segregating progenies displaying opposite architecture features were used to link phenotype to expression. Here, we identify 2252 differentially expressed genes (DEGs) associated to differences in plant architecture. Microarray results were validated by quantitative RT-PCR carried out on genes with functional annotation likely related to plant architecture. Twelve of these genes were further analyzed in individual seedlings of the corresponding pool. We also examined Arabidopsis mutants in putative orthologs of these targeted candidate genes, finding altered architecture for most of them. This supports a functional conservation between species and potential biological relevance of the candidate genes identified. This study is the first to identify genes associated to plant architecture in olive, and the results obtained could be of great help in future programs aimed at selecting phenotypes adapted to modern cultivation practices in this species.

  9. Post-weaning feed efficiency decreased in progeny of higher milk yielding beef cows.

    Science.gov (United States)

    Mulliniks, J T; Edwards, S R; Hobbs, J D; McFarlane, Z D; Cope, E R

    2018-02-01

    Current trends in the beef industry focus on selecting production traits with the purpose of maximizing calf weaning weight; however, such traits may ultimately decrease overall post-weaning productivity. Therefore, the objective of this study was to evaluate the effects of actual milk yield in mature beef cows on their offspring's dry matter intake (DMI), BW, average daily gain, feed conversion ratio (FCR) and residual feed intake (RFI) during a ~75-day backgrounding feeding trial. A period of 24-h milk production was measured with a modified weigh-suckle-weigh technique using a milking machine. After milking, cows were retrospectively classified as one of three milk yield groups: Lower (6.57±1.21 kg), Moderate (9.02±0.60 kg) or Higher (11.97±1.46 kg). Calves from Moderate and Higher milk yielding dams had greater (Pfeeding phase; however, day 75 BW were not different (P=0.36) between Lower and Moderate calves. Body weight gain was greater (P=0.05) for Lower and Moderate calves from the day 0 BW to day 35 BW compared with Higher calves. Overall DMI was lower (P=0.03) in offspring from Lower and Moderate cows compared with their Higher milking counterparts. With the decreased DMI, FCR was lower (P=0.03) from day 0 to day 35 in calves from Lower and Moderate milk yielding dams. In addition, overall FCR was lower (P=0.02) in calves from Lower and Moderate milk yielding dams compared with calves from Higher milk yielding dams. However, calving of Lower milk yielding dams had an increased (P=0.04) efficiency from a negative RFI value compared with calves from Moderate and Higher milking dams. Results from this study suggest that increased milk production in beef cows decreases feed efficiency during a 75-day post-weaning, backgrounding period of progeny.

  10. Effect of strenuous maternal exercise before and during pregnancy on rat progeny renal function

    Directory of Open Access Journals (Sweden)

    Oliveira A.O.

    2004-01-01

    Full Text Available The effects of strenuous exercise before and during pregnancy on the renal function and morphological alterations of the progeny were determined in a study on female Wistar rats. This research was done based on a previous study carried out in our laboratory, which showed morphological alterations in rats submitted to this kind of exercise. As the form is related to the function, the physiological relevance of submitting a pregnant female to a high-intensity exercise training regimen could be explained by the fact that morphological alterations can influence kidney function. The animals were assigned to one of two groups: control animals that did not exercise during pregnancy and trained animals that swam for 120 min 5 days a week for 8 weeks before pregnancy and daily for 60 min over a period of 8 weeks starting on the second day of pregnancy. Seven rats of each group were analyzed for morphological alterations and for renal function. The progeny of the rats used for morphological evaluation were born by cesarean section and the progeny of the animals used to evaluate renal function were born normally. The progeny were two months old when renal function was evaluated. Fertility and morbidity were the same for both groups. Strenuous maternal exercise had no significant influence on glomerular filtration rate (GFR but renal plasma flow was lower in the progeny of the trained group (mean ± SD, 16.65 ± 3.77 ml min-1 kg-1 compared to the progeny of the control group (33.42 ± 2.56 ml min-1 kg-1. Antidiuretic and antinatriuretic effects on the progeny of the trained group were observed, since urine flow as percentage of GFR and the fraction of urinary sodium excretion were lower in this group (1.38 ± 0.10 and 0.60 ± 0.04%, respectively compared to the progeny of the control group (2.36 ± 0.11 and 1.55 ± 0.20%, respectively. Moreover, in this exercise program, fetuses from trained animals were small-sized (2.45 ± 0.19 vs 4.66 ± 2.45 g for

  11. A family business: stem cell progeny join the niche to regulate homeostasis.

    Science.gov (United States)

    Hsu, Ya-Chieh; Fuchs, Elaine

    2012-01-23

    Stem cell niches, the discrete microenvironments in which the stem cells reside, play a dominant part in regulating stem cell activity and behaviours. Recent studies suggest that committed stem cell progeny become indispensable components of the niche in a wide range of stem cell systems. These unexpected niche inhabitants provide versatile feedback signals to their stem cell parents. Together with other heterologous cell types that constitute the niche, they contribute to the dynamics of the microenvironment. As progeny are often located in close proximity to stem cell niches, similar feedback regulations may be the underlying principles shared by different stem cell systems.

  12. Recurrent Infections May Signal Immunodeficiencies

    Science.gov (United States)

    ... Recurrent Infections May Signal Immunodeficiencies Share | Recurrent Infections May Signal Immunodeficiencies This article has been reviewed by ... common bacterial infections? If so, these recurrent infections may be a sign of an immunodeficiency disorder. The ...

  13. Progestagen therapy for recurrent miscarriage

    National Research Council Canada - National Science Library

    Szekeres-Bartho, Julia; Balasch, Juan

    .... METHODS We searched PubMed and the Cochrane database covering the period of 1968-2007. The search terms progestogens and recurrent miscarriage, NK cells and recurrent miscarriage as well as cytokines and recurrent miscarriage were used...

  14. Management of recurrent intussusception: nonoperative or operative reduction?

    Science.gov (United States)

    Niramis, Rangsan; Watanatittan, Sukawat; Kruatrachue, Anchalee; Anuntkosol, Maitree; Buranakitjaroen, Veera; Rattanasuwan, Tongkao; Wongtapradit, Lawan; Tongsin, Achariya

    2010-11-01

    The aim of this study was to determine how to manage children with recurrence of intussusception. Medical records of patients treated for intussusception from 1976 to 2008 at the Queen Sirikit National Institute of Child Health were reviewed. Information on patients who developed recurrent intussusception was extracted to study patterns of recurrent attack and suitable management procedures. The statistical differences were analyzed by the χ² and the Student t test, with a P value lead point: colonic polyps in 4 cases and Meckel diverticulum in 3 cases. There were no deaths among the 75 patients with recurrent intussusception. Recurrent intussusception should be initially treated by nonoperative reduction. Laparotomy is needed in cases with failure of BE or AE reduction, in cases with suspicion of a pathologic lead point, and in selected cases with several episodes of recurrence. The treatment of recurrent intussusception, in general, should be similar to that of primary intussusception. Copyright © 2010 Elsevier Inc. All rights reserved.

  15. RECURRENT CROUP IN CHILDREN

    Directory of Open Access Journals (Sweden)

    S. L. Piskunova

    2014-01-01

    Full Text Available The article presents the results of examination of 1849 children, entering children's infectioushospitalofVladivostokwith the clinical picture of croup of viral etiology. The clinical features of primary and recurrent croup are described. Frequency of recurrent croup inVladivostokis 8%. Children with a recurrent croup had the burdened premorbid background, and also persistent herpetic infections (cytomegalic infection in 42,9% cases, cytomegalic infection in combination with the herpes simplex virus -1. Frequency of croups substantially rose in the period of epidemic of influenza.

  16. Growth and Crown Vigor of 25 Year-Old Shortleaf Pine Progenies on a Littleleaf Disease Site

    Science.gov (United States)

    Stanley J. Zarnoch; John L. Ruehle; Roger P. Belanger; Donald H. Marx; W. Craig Bryan

    1994-01-01

    On a littleleaf disease site in South Carolina, most of the control-pollinated progeny of shrotleaf pines that appeared to be reesistant to the disease outperformed a check seedlot through age 25. Rankings of progeny based on volume changed little between ages 17 and 25.

  17. Selection arena in Aspergillus nidulans

    NARCIS (Netherlands)

    Bruggeman, J.; Debets, A.J.M.; Hoekstra, R.F.

    2004-01-01

    The selection arena hypothesis states that overproduction of zygotes-a widespread phenomenon in animals and plants-can be explained as a mechanism of progeny choice. As a similar mechanism, the ascomycetous fungus Aspergillus nidulans may overproduce dikaryotic fruit initials, hereafter called

  18. Recurrent Intracerebral Hemorrhage

    DEFF Research Database (Denmark)

    Schmidt, Linnea Boegeskov; Goertz, Sanne; Wohlfahrt, Jan

    2016-01-01

    treatment and renal insufficiency were associated with increased recurrence risks (RR 1.64, 95% CI 1.39-1.93 and RR 1.72, 95% CI 1.34-2.17, respectively), whereas anti-hypertensive treatment was associated with a reduced risk (RR 0.82, 95% CI 0.74-0.91). We observed non-significant associations between...... reuptake inhibitors (SSRI's), and nonsteroidal anti-inflammatory drugs (NSAID's) with recurrent ICH. METHODS: The cohort consisted of all individuals diagnosed with a primary ICH in Denmark 1996-2011. Information on comorbidities, surgical treatment for the primary ICH, and the use of ATT, SSRI's and NSAID...... the use of any of the investigated medicines with antithrombotic effect (ATT, SSRI's, NSAID's) and recurrent ICH. CONCLUSIONS: The substantial short-and long-term recurrence risks warrant aggressive management of hypertension following a primary ICH, particularly in patients treated surgically...

  19. Immunomodulation in Recurrent Miscarriage

    National Research Council Canada - National Science Library

    Kumar, Ashok

    2014-01-01

    ... % of women with recurrent miscarriage (RM). Several studies demonstrated that successful pregnancy is dependant on shifting of maternal immune response from (proinflammatory) Th1 toward (anti-inflammatory) Th2 phenotypes...

  20. QTL analysis of the genetic architecture determining resistance to fire blight in an apple progeny

    NARCIS (Netherlands)

    Calenge, F.; Drouet, D.; Weg, van de W.E.; Brisset, M.N.; Paulin, J.P.; Durel, C.E.

    2004-01-01

    Fire blight, caused by the bacterial pathogen Erwinia amylovora, is one of the most destructive diseases of apple (Malus x domestica). In order to analyse the genetic determinism of resistance to fire blight in apple, a quantitative trait analysis (QTL) approach was used. A F1 progeny of 164

  1. Past as Prologue: Educational Psychology's Legacy and Progeny

    Science.gov (United States)

    Alexander, Patricia A.

    2018-01-01

    On the occasion of the 125th anniversary of the American Psychological Association, the legacies and progenies of the discipline of educational psychology are explored. To capture those legacies, transformational and influential contributions by educational psychologists to schools and society are described as key themes. Those themes entail: the…

  2. Oleoresin characteristics of progeny of loblolly pines that escaped attack by southern pine beetle

    Science.gov (United States)

    B.L. Strom; R.A. Goyer; L.L. Ingram; G.D.L. Boyd; L.H. Lott

    2002-01-01

    Oleoresin characteristics of first-generation (F1) progeny of loblolly pines (Pinus taeda L.) that escaped mortality from the southern pine beetle, Dendroctonus frontalis Zimmermann (Coleoptera: Scolytidae), despite heavy mortality of neighbors, were evaluated and compared to trees from a general (i.e., trees...

  3. Immunological surveillance in a pseudorabies quarantined herd using gilts and their progeny as sentinels.

    Science.gov (United States)

    Williams, P P; Sacks, J M; Yang, P C; Pirtle, E C; Erickson, G A; Beran, G W

    1989-01-01

    Specific pathogen free gilts and their progeny were evaluated to use as sentinels in a pseudorabies virus (PRV) infected herd by immunologically monitoring for PRV seroconversions. Time intervals targeted were pre- and post-PRV vaccinations, herd exposure, and farrowing to finishing. Post-PRV vaccinations, gilts showed low PRV lymphocyte stimulation and humoral responses. Following herd exposure, control gilts PRV seroconverted and PRV vaccinated gilts increased (2 to 4 times) in virus neutralization (VN) titers. Sixty-seven percent (4/6) of the progeny from a control gilt were PRV seropositive at finishing. Progeny from PRV vaccinated gilts were depleted of passive immunity by week 7, and were seronegative until week 9. At finishing 47% (14/30) of them were PRV seropositive indicating exposure to PRV. The VN test was not sensitive enough to detect weak positive serums, noted as positives by latex agglutination (LA) test, ELISA, and Western blots. The gilts and progeny detected PRV, respectively, in the herd housing quarters and in the farrow to finish facilities. A strategy for future sentinel experimental surveillances using primarily the LA test is proposed.

  4. Inherent Variation Among Slash Pine Progenies at the Ida Cason Callaway Foundation

    Science.gov (United States)

    John C. Barber

    1964-01-01

    Reported here in detail is information obtained from two open-pollinated progeny tests of slash pine at the Ida Cason Callaway Foundation, Pine Mountain, Georgia. Because of the small amount of similar data available to tree improvement workers, it was decided to include as much information as possible, even though some of it is too limited for statistical analyses...

  5. Right of Progeny and Cairo Declaration of Human Rights in Islam

    Directory of Open Access Journals (Sweden)

    Haleema Ashfaq

    2017-01-01

    Full Text Available Abstract The first main objective of Maqasid I Shari`ah is the completion of human’s necessity; in which protection of progeny (nasl is the foremostpurpose. The preservation of lineage is greatly emphasized by the Islamic Shariah and the Cairo declaration of human rights in Islam also supported the protection of lineage in Islam. All the articles of CDHRI covered the five basic human rights mentioned in Maqasid I Shariah. The research is focused on delineating the concept of protection of lineage as one of the main objective of Shariah and it is supported by evidences from Cairo Declaration of Human Rights in Islam along with Quranic verses and traditions of the Holy Prophet (S.A.W. The aspects covered in the paper range from discussion of right of progeny as well as equal rights of progeny for male and female; rights of children with the hierarchy of their rights from the stage of fetus, having proper nursing, caring, education and a healthy beginning of life. The comparative analysis based on arguments of Shariah and the CDHRI proves that it is the basic objective of Shariah to protect all fundamental rights and right of progeny has the foremost significance in it.

  6. Prediction of lung cells oncogenic transformation for induced radon progeny alpha particles using sugarscape cellular automata.

    Science.gov (United States)

    Baradaran, Samaneh; Maleknasr, Niaz; Setayeshi, Saeed; Akbari, Mohammad Esmaeil

    2014-01-01

    Alpha particle irradiation from radon progeny is one of the major natural sources of effective dose in the public population. Oncogenic transformation is a biological effectiveness of radon progeny alpha particle hits. The biological effects which has caused by exposure to radon, were the main result of a complex series of physical, chemical, biological and physiological interactions. The cellular and molecular mechanisms for radon-induced carcinogenesis have not been clear yet. Various biological models, including cultured cells and animals, have been found useful for studying the carcinogenesis effects of radon progeny alpha particles. In this paper, sugars cape cellular automata have been presented for computational study of complex biological effect of radon progeny alpha particles in lung bronchial airways. The model has included mechanism of DNA damage, which has been induced alpha particles hits, and then formation of transformation in the lung cells. Biomarkers were an objective measure or evaluation of normal or abnormal biological processes. In the model, the metabolism rate of infected cell has been induced alpha particles traversals, as a biomarker, has been followed to reach oncogenic transformation. The model results have successfully validated in comparison with "in vitro oncogenic transformation data" for C3H 10T1/2 cells. This model has provided an opportunity to study the cellular and molecular changes, at the various stages in radiation carcinogenesis, involving human cells. It has become well known that simulation could be used to investigate complex biomedical systems, in situations where traditional methodologies were difficult or too costly to employ.

  7. Assessing the Potential Oleoresin Yields of Slash Pine Progenies at Juvenile Ages

    Science.gov (United States)

    A.E. Squillace; Charles R. Gansel

    1968-01-01

    The potential oleoresin yields of slash pine progenies can be assessed at juvenile ages, 7 to 8 years earlier than with previous methods. Seeds are sown in peat pots, outplanted shortly after germination at a spacing of 14 by 3 feet, and given intensive cultural treatment. At 26 years from seed, when the trees average about 9 feet tall, their potential yields are...

  8. Dietary manipulation of the sow milk does not influence the lipid absorption capacity of the progeny

    DEFF Research Database (Denmark)

    Lauridsen, Charlotte; Hedemann, Mette Skou; Pierzynowski, Stefan

    2007-01-01

    A control diet without supplemental fat and four diets containing 8% of coconut oil, rapeseed oil, fish oil or sunflower oil were fed to lactating sows in order to investigate the lipid absorption capacity of their progeny in terms of pancreatic enzyme activity, hormonal regulation, and bile salt...

  9. Replication of mycoplasmavirus MVL51 III identification of a progeny viral DNA-protein intermediate

    Energy Technology Data Exchange (ETDEWEB)

    Das, J.; Maniloff, J.

    1976-01-01

    Intracellular replication of the non-lytic single stranded circular DNA mycoplasmavirus MVL51 has been shown to involve three DNA intermediates: RFI, RFII and SSI. Growth in Eagle's basal medium, rather than richer tryptose medium, has allowed the identification of an intermediate between nascent progeny chromosomes (SSI) and mature virus. This intermediate is a protein associated form of SSI.

  10. Multifocal recurrent periostitis

    Energy Technology Data Exchange (ETDEWEB)

    Kozlowski, K.; Anderson, R.; Tink, A.

    1981-11-01

    Two case reports of recurrent multifocal periostitis in two girls aged 15 and 16 are added to the eight cases already reported in the literature. The disease is characterised clinically by recurrent mesomelic swelling of the extremities and radiologically by periosteal thickening and sclerosis of underlying bone. Hyperglobulinaemia is the most constant biochemical finding. The bone biopsy shows no typical features. The possibility of a viral etiology is discussed.

  11. Growth, reproductive performance, carcass characteristics and meat quality in F1 and F2 progenies of somatic cell-cloned pigs.

    Science.gov (United States)

    Adachi, Noritaka; Yamaguchi, Daisuke; Watanabe, Akiyuki; Miura, Narumi; Sunaga, Seiji; Oishi, Hitoshi; Hashimoto, Michiko; Oishi, Takatsugu; Iwamoto, Masaki; Hanada, Hirofumi; Kubo, Masanori; Onishi, Akira

    2014-04-24

    The objective of this study was to examine the health and meat production of cloned sows and their progenies in order to demonstrate the application of somatic cell cloning to the pig industry. This study compared the growth, reproductive performance, carcass characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies. We measured their body weight, growth rate and feed conversion and performed a pathological analysis of their anatomy to detect abnormalities. Three of the five cloned pigs were used for a growth test. Cloned pigs grew normally and had characteristics similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a Landrace boar and used for a progeny test. F1 progenies had characteristics similar to those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White boar and used for the progeny test. F2 progenies grew normally. There were no biological differences in growth, carcass characteristics and amino acid composition among cloned sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies showed normal reproductive performance. No specific abnormalities were observed by pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs had a normal karyotype. These results demonstrate that cloned female pigs and their progenies have similar growth, reproductive performance and carcass quality characteristics and that somatic cell cloning could be a useful technique for conserving superior pig breeds in conventional meat production.

  12. A phase II evaluation of selumetinib (AZD6244, ARRY-142886), a selective MEK-1/2 inhibitor in the treatment of recurrent or persistent endometrial cancer: An NRG Oncology/Gynecologic Oncology Group study

    Science.gov (United States)

    Coleman, Robert L.; Sill, Michael W.; Thaker, Premal H.; Bender, David P.; Street, Daron; McGuire, William; Johnston, Carolyn M.; Rotmensch, Jacob

    2015-01-01

    Background Activation of the mitogen activated protein kinase pathway plays a pivotal role in cell proliferation and is frequently activated in endometrial cancer. We sought to evaluate the efficacy/safety of selumetinib, a selective MEK-1/2 inhibitor in women with recurrent endometrial cancer. Methods This was a phase II, single-arm, open-label study evaluating response and 6-month event-free survival (EFS) as primary endpoints. Eligible patients had measurable disease, 1–2 prior cytotoxic regimens, and performance status 0–2. Selumetinib 75 mg PO BID was administered daily until progression or intolerance. One cycle was 28 days. Results Fifty-four patients were enrolled; 2 were excluded due to improper pre-study treatment (1) and never treated (1), leaving 52 evaluable for efficacy/safety. Median age was 62; histology included endometrioid (58%), serous (17%) and mixed (23%). Seventeen patients (33%) had 2 prior cytotoxic regimens. The median number of cycles administered was 2 (1–34). Three (6%) patients had objective response (1 CR, 2 PR); 13 had SD as best response. The proportion of patients with 6-month EFS was 12%. Median EFS, progression-free and overall survival was 2.1, 2.3, and 8.5 months, respectively. Drug-attributed grade 3/4 adverse events were observed (≥ 5%) were fatigue (15%), anemia (10%), pain (10%), extremity edema (8%), and dyspnea (6%). There was 1 grade 4 infection (renal), 1 grade 4 anemia, and 1 death due to hemorrhage (rectum). Conclusions Selumetinib was tolerable in this population but did not meet pre-trial specifications for clinical efficacy. PMID:25887099

  13. Hybrid Weakness Found in the Backcross Progeny of an Inter-Specific Cross of Oryza Sativa / O. Glumaepatula

    Directory of Open Access Journals (Sweden)

    Sobrizal

    2009-01-01

    Full Text Available Hybrid breakdown expressed as sterility or weakness or both in F2 or later generations is considered to be a reproductive barrier between two distantly related taxa. Hybrid breakdown that showed poor growth habit with fertile seeds was found in the backcross progenies (BC4F2 which were derived from a cross between a cultivated rice Oryza sativa L. cv. Taichung 65 and a wild rice Oryza glumaepatula Steud (Acc. IRGC 105668 where Taichung 65 was used as a recurrent parent. Through segregation analysis of BC4F2 population, a novel gene for weakness was identified, and this gene was designated as hwf1 (hybrid weakness f-1. RFLP (Restriction Fragment Length Polymorphism analysis was employed to determine the chromosomal location of hwf1 gene. The result revealed that hwf1 was located between G3006 and C933 RFLP markers, with map distances of 0.5 cM and 1.9 cM, respectively, and co-segregated with C708, C820, and R288 on the short arm of chromosome 4. Since both O. glumaepatula (Acc. IRGC 105668 and Taichung 65 that was used as parents showed normal growth habit, the weakness in this study was assumed to be controlled by a set of complementary recessive genes. One of the complementary genes is hwf1 from O. glumaepatula, and another gene should be from Taichung 65, because the genetic background of the BC4F2 populations that were used for analysis is Taichung 65. The gene from Taichung 65 has not been identified yet, therefore, it is necessary to conduct the experiment for finding out the complement of hwf1 gene in near future.

  14. Recurrent Fever in Children

    Directory of Open Access Journals (Sweden)

    Sofia Torreggiani

    2016-03-01

    Full Text Available Children presenting with recurrent fever may represent a diagnostic challenge. After excluding the most common etiologies, which include the consecutive occurrence of independent uncomplicated infections, a wide range of possible causes are considered. This article summarizes infectious and noninfectious causes of recurrent fever in pediatric patients. We highlight that, when investigating recurrent fever, it is important to consider age at onset, family history, duration of febrile episodes, length of interval between episodes, associated symptoms and response to treatment. Additionally, information regarding travel history and exposure to animals is helpful, especially with regard to infections. With the exclusion of repeated independent uncomplicated infections, many infective causes of recurrent fever are relatively rare in Western countries; therefore, clinicians should be attuned to suggestive case history data. It is important to rule out the possibility of an infectious process or a malignancy, in particular, if steroid therapy is being considered. After excluding an infectious or neoplastic etiology, immune-mediated and autoinflammatory diseases should be taken into consideration. Together with case history data, a careful physical exam during and between febrile episodes may give useful clues and guide laboratory investigations. However, despite a thorough evaluation, a recurrent fever may remain unexplained. A watchful follow-up is thus mandatory because new signs and symptoms may appear over time.

  15. THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE

    Directory of Open Access Journals (Sweden)

    Satoto Satoto

    2016-10-01

    Full Text Available Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2 of fivetransgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. Data  resulted from PCR of F2 population were analysed using a Chi Square test. The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of genetic sources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer or  separately in rice breeding programs whenever

  16. Efeito da conexidade de dados sobre a acurácia dos testes de progênie e performance Data connectedness effect on the accuracy of progeny and performance tests

    Directory of Open Access Journals (Sweden)

    Antonio Policarpo Souza Carneiro

    2001-04-01

    Full Text Available Dados simulados foram utilizados para verificar o efeito da conexidade sobre a acurácia dos testes de progênie e performance. O genoma simulado foi constituído de uma característica quantitativa governada por 500 locos. Simulou-se o efeito de rebanho (9 rebanhos significativo a 5% de probabilidade pelo teste F. Foram simulados arranjos de dados com 0, 15, 30, 60, 90 e 100% de conexidade para herdabilidades 0,10; 0,30; e 0,60 e para diferentes tamanhos de progênie (9, 54 e 90 progênies/reprodutor. A cada geração foram selecionados nove machos e o número de fêmeas selecionadas variou de acordo com o número de progênies considerado em cada arranjo de dados. A seleção foi efetuada em dez gerações, sendo este processo repetido por 30 vezes. O uso de dados com baixa conexidade reduziu a acurácia dos testes considerados, e a maior importância da conexidade ocorreu para baixa herdabilidade e reduzido tamanho da progênie. Apesar de ter sido observado efeito da conexidade, a herdabilidade e o tamanho da progênie tiveram maior influência na avaliação genética.Simulated data were used to verify the effect of the connectedness on the accuracy of progeny and performance tests. The simulated genome was formed from a quantitative trait governed by 500 loci. Significance herd effect (9 herds by 5% probability F test was simulated. Data sets with 0, 15, 30, 60, 90 and 100% of connectedness for heritabilities 0.10, 0.30 and 0.60 and for different progeny sizes (9, 54 and 90 progenies/sire were simulated. In each generation nine males were selected and the number of selected females shifted according to the number of progenies considering in each data arrange. The selection was accomplished in ten generations, where the process was repeated 30 times. Data with smaller connectedness reduced the accuracy of both tests and the connectedness was more important for small heritability and small progeny size. The heritability and the progeny size

  17. Electrical capacitance as a predictor of root dry weight in shrub willow (Salix; Salicaceae) parents and progeny.

    Science.gov (United States)

    Carlson, Craig H; Smart, Lawrence B

    2016-08-01

    Root biomass is an important trait often disregarded in woody perennial selection due to the challenge and expense of accurately and efficiently measuring large populations. In this study, we aim to develop a simple method that can predict root dry weight within a diverse shrub willow (Salix) breeding population representing species hybrids and their parents using root electrical capacitance (REC). The REC method was tested on plants started from cuttings and grown in pots with potting mix in the greenhouse for 11 wk to assess the relationship of REC with 24 biomass traits and its usefulness in allometric models for root and stem dry biomass. Strong linear and positive correlations were found between REC and root dry biomass (r = 0.88). The total proportion of variance of root and stem dry biomass explained by predictors in multiple regression was 85% and 69%, respectively. The relative importance of predictor variables in allometric models was dominated by the contribution of REC. This work provides an efficient and nondestructive technique to indirectly quantify root biomass of genetically diverse shrub willow progeny, which has great promise for selection of genotypes with varying root biomass and for the accurate estimation of belowground carbon sequestration.

  18. Genomic approaches to selection in outcrossing perennials: focus on essential oil crops.

    Science.gov (United States)

    Kainer, David; Lanfear, Robert; Foley, William J; Külheim, Carsten

    2015-12-01

    The yield of essential oil in commercially harvested perennial species (e.g. 'Oil Mallee' eucalypts, Tea Trees and Hop) is dependent on complex quantitative traits such as foliar oil concentration, biomass and adaptability. These often show large natural variation and some are highly heritable, which has enabled significant gains in oil yield via traditional phenotypic recurrent selection. Analysis of transcript abundance and allelic diversity has revealed that essential oil yield is likely to be controlled by large numbers of quantitative trait loci that range from a few of medium/large effect to many of small effect. Molecular breeding techniques that exploit this information could increase gains per unit time and address complications of traditional breeding such as genetic correlations between key traits and the lower heritability of biomass. Genomic selection (GS) is a technique that uses the information from markers genotyped across the whole genome in order to predict the phenotype of progeny well before they reach maturity, allowing selection at an earlier age. In this review, we investigate the feasibility of genomic selection (GS) for the improvement of essential oil yield. We explore the challenges facing breeders selecting for oil yield, and how GS might deal with them. We then assess the factors that affect the accuracy of genomic estimated breeding values, such as linkage disequilibrium (LD), heritability, relatedness and the genetic architecture of desirable traits. We conclude that GS has the potential to significantly improve the efficiency of selection for essential oil yield.

  19. Ethanol Consumption by Wistar Rat Dams Affects Selenium Bioavailability and Antioxidant Balance in Their Progeny

    Directory of Open Access Journals (Sweden)

    Olimpia Carreras

    2009-07-01

    Full Text Available Ethanol consumption affects maternal nutrition, the mothers’ antioxidant balance and the future health of their progeny. Selenium (Se is a trace element cofactor of the enzyme glutathione peroxidase (GPx. We will study the effect of ethanol on Se bioavailability in dams and in their progeny. We have used three experimental groups of dams: control, chronic ethanol and pair-fed; and three groups of pups. Se levels were measured by graphite-furnace atomic absorption spectrometry. Serum and hepatic GPx activity was determined by spectrometry. We have concluded that ethanol decreased Se retention in dams, affecting their tissue Se deposits and those of their offspring, while also compromising their progeny’s weight and oxidation balance. These effects of ethanol are caused by a reduction in Se intake and a direct alcohol-generated oxidation action.

  20. Behavior of 222Rn and its progeny in high-rise buildings.

    Science.gov (United States)

    Leung, J K; Tso, M Y; Ho, C W

    1998-09-01

    Unlike detached or semi-detached houses, the main source of indoor radon in high-rise buildings is the building material. Radon released from the building material will be removed by ventilation, either forced or natural, so that its concentration, its progeny's concentration, and the equilibrium between the two will be different for different types of buildings and environmental factors. A number of surveys were carried out in buildings throughout the territory to look at the seasonal variation of indoor 222Rn levels; the dependence of indoor 222Rn concentration on the living environment; the indoor gamma dose rate and its relation to indoor 222Rn concentration; and the dependence of 222Rn progeny concentration and equilibrium factor on the environment in high-rise buildings.

  1. Early flowering and seed production in a yellow birch progeny test

    Science.gov (United States)

    Knud E. Clausen

    1976-01-01

    Trees in a yellow birch progeny test began to bear seed when 7 years old and the proportion of fruiting trees increased in the following 2 years. Male catkins were produced at age 8 and the number of trees with males increased greatly the following years. Although there is much variation between and within families in earliness of flowering and in number of flowers and...

  2. Laparoscopic Surgery for Recurrent Crohn's Disease

    Directory of Open Access Journals (Sweden)

    Antonino Spinelli

    2012-01-01

    Full Text Available In spite of the recent improvements in drug therapy, surgery still represents the most frequent treatment for Crohn's disease (CD complications. Laparoscopy has been widely applied over the last twenty years in colorectal surgery and was associated with lower postoperative pain, shorter hospitalization, faster return to daily activities, and better cosmetic results. Laparoscopy experienced a slower diffusion in inflammatory bowel disease surgery than in oncologic colorectal surgery, but proved to be safe and effective, and is currently considered the gold standard for the treatment of primary uncomplicated ileocolic CD. Indications for laparoscopy in CD have recently been widened to embrace more complicated or recurrent CD. This paper reviews the available data on the subset of recurrent CD patients. The reported results indicate that laparoscopy may be safely applied even in selected recurrent CD cases in hands of IBD surgeons with broad laparoscopic experience.

  3. On Solving Linear Recurrences

    Science.gov (United States)

    Dobbs, David E.

    2013-01-01

    A direct method is given for solving first-order linear recurrences with constant coefficients. The limiting value of that solution is studied as "n to infinity." This classroom note could serve as enrichment material for the typical introductory course on discrete mathematics that follows a calculus course.

  4. Recurrences of strange attractors

    Indian Academy of Sciences (India)

    order to detect the transitions from or to SNAs too. The outline of this paper is as follows: in §2, we present the recurrence approach to detect the different transitions. This approach is applied in §3 to detect transi- tions to SNAs in the quasiperiodically forced logistic map. Section 4 examines the transition from SNAs to chaos.

  5. Recurrent diabetic ketoacidosis

    DEFF Research Database (Denmark)

    Skinner, T. Chas

    2002-01-01

    Longitudinal studies indicate that 20% of paediatric patients account for 80% of all admissions for diabetic ketoacidosis (DKA). The frequency of DKA peaks during adolescence and, although individuals generally go into remission, they may continue to have bouts of recurrent DKA in adulthood. The ...

  6. Training Recurrent Networks

    DEFF Research Database (Denmark)

    Pedersen, Morten With

    1997-01-01

    Training recurrent networks is generally believed to be a difficult task. Excessive training times and lack of convergence to an acceptable solution are frequently reported. In this paper we seek to explain the reason for this from a numerical point of view and show how to avoid problems when...

  7. Recurrent Spatial Transformer Networks

    DEFF Research Database (Denmark)

    Sønderby, Søren Kaae; Sønderby, Casper Kaae; Maaløe, Lars

    2015-01-01

    We integrate the recently proposed spatial transformer network (SPN) [Jaderberg et. al 2015] into a recurrent neural network (RNN) to form an RNN-SPN model. We use the RNN-SPN to classify digits in cluttered MNIST sequences. The proposed model achieves a single digit error of 1.5% compared to 2...

  8. Chronic recurrent multifocal osteomyelitis

    NARCIS (Netherlands)

    Wedman, Jan; van Weissenbruch, Ranny

    We report what is, to our best knowledge, the first case of chronic recurrent multifocal osteomyelitis (CRMO) in which the frontal and sphenoid bones were involved. Characterized by a prolonged and fluctuating course of osteomyelitis at different sites, CRMO is self-limited, although sequelae can

  9. Lung Cancer Indicators Recurrence

    Science.gov (United States)

    This study describes prognostic factors for lung cancer spread and recurrence, as well as subsequent risk of death from the disease. The investigators observed that regardless of cancer stage, grade, or type of lung cancer, patients in the study were more

  10. Analysis of radon and thoron progeny measurements based on air filtration.

    Science.gov (United States)

    Stajic, J M; Nikezic, D

    2015-02-01

    Measuring of radon and thoron progeny concentrations in air, based on air filtration, was analysed in order to assess the reliability of the method. Changes of radon and thoron progeny activities on the filter during and after air sampling were investigated. Simulation experiments were performed involving realistic measuring parameters. The sensitivity of results (radon and thoron concentrations in air) to the variations of alpha counting in three and five intervals was studied. The concentration of (218)Po showed up to be the most sensitive to these changes, as was expected because of its short half-life. The well-known method for measuring of progeny concentrations based on air filtration is rather unreliable and obtaining unrealistic or incorrect results appears to be quite possible. A simple method for quick estimation of radon potential alpha energy concentration (PAEC), based on measurements of alpha activity in a saturation regime, was proposed. Thoron PAEC can be determined from the saturation activity on the filter, through beta or alpha measurements. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  11. Generation of viable progeny from dead brooders of endangered catfish Clarias magur (Hamilton, 1822

    Directory of Open Access Journals (Sweden)

    Sullip Kumar Majhi

    2017-10-01

    Full Text Available The obligatory air-breathing catfish Clarias magur is a prime candidate for aquaculture owing to its unique taste, high growth rate, and hardy nature. However, recently the IUCN has listed the species under the endangered category because the population has critically declined in the wild. The sexually mature C. magur brooders are often collected from their natural habitats for seed production in captivity. In many cases, the brooder dies due to handling injuries or confinement stress. In this study, we demonstrated that viable progeny could be generated from freshly dead sexually mature C. magur. Three hours after death, the gonads were excised, macroscopically examined and gamete viability was evaluated. Artificial fertilization was performed by mixing the sperm suspension with the eggs. Water was added after 1 min of mixing to activate the fertilization process. We observed 85%-93% fertilization success from gametes derived from dead donors as opposed to 90%-95% from those derived from live control donors. The embryos showed normal development and resulted in the generation of 88%-92% viable progeny, which was similar to the progeny derived from control donors (92%-93%. The results obtained in this study will have profound implications in enhancing the seed production of endangered C. magur and could potentially be applied to other key commercially or endangered fish species. Keywords: Biological sciences, Developmental biology, Zoology

  12. Polyphenol variability in the fruits and juices of a cider apple progeny.

    Science.gov (United States)

    Verdu, Cindy F; Childebrand, Nicolas; Marnet, Nathalie; Lebail, Gildas; Dupuis, Fabrice; Laurens, François; Guilet, David; Guyot, Sylvain

    2014-05-01

    Polyphenols have a favourable antioxidant potential on human health, suggesting that their high content in apple is responsible for the beneficial effects of apple consumption. They are also linked to the quality of apple juices and ciders since they are predominantly responsible for astringency, bitterness and colour. Major phenolic compounds were quantified by liquid chromatography in fruits and juices from a cider apple progeny harvested for 3 years. The total content of procyanidins and their average degree of polymerisation (DPn) were also determined in fruits by phloroglucinolysis. Variability and extraction yield of these compounds were determined. The variability observed in the progeny was representative of the variability observed in many cider apple varieties. Hydroxycinnamic acids were the most extractable group, with an average extraction yield of 67%, whereas flavonols and anthocyanins were the least. This study is the first to introduce variability and extraction yields of the main phenolic compounds in both fruits and juices of a cider apple progeny. This dataset will be used for an upcoming QTL mapping study, an original approach that has never been undertaken for cider apple. © 2013 Society of Chemical Industry.

  13. Study on the influence of CR-39 detector size on radon progeny detection in indoor environments

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, L. A.; Hadler, J. C.; Lixandrão F, A. L.; Guedes, S.; Takizawa, R. H. [Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, UNICAMP, 13083-970 Campinas, SP (Brazil)

    2014-11-11

    It is well known that radon daughters up to {sup 214}Po are the real contaminants to be considered in case of indoor radon contamination. Assemblies consisting of 6 circular bare sheets of CR-39, a nuclear track detector, with radius varying from 0.15 to 1.2 cm were exposed far from any material surface for periods of approximately 6 months in 13 different indoor rooms (7 workplaces and 6 dwellings), where ventilation was moderate or poor. It was observed that track density was as greater as smaller was the detector radius. Track density data were fitted using an equation deduced based on the assumption that the behavior of radon and its progeny in the air was described by Fick's Law, i.e., when the main mechanism of transport of radon progeny in the air is diffusion. As many people spend great part of their time in closed or poorly ventilated environments, the confirmation they present equilibrium between radon and its progeny is an interesting start for dosimetric calculations concerning this contamination.

  14. Reduction of radon progeny concentration in ordinary room due to a mixing fan

    Energy Technology Data Exchange (ETDEWEB)

    Abu-Jarad, F.; Sextro, R.G.

    1988-01-01

    Three experiments were carried out at the Indoor Air Quality Research House (IAQRH) at Lawrence Berkeley Laboratory, University of California, to study the effect on the radon progeny of operating a mixing fan. At the beginning of every experiment, about 15 kBq.m/sup -3/ of radon was injected into a 36 m/sup 3/ ordinary room. Measurements were conducted with three different aerosol particle concentrations of about 10/sup 2/, 10/sup 4/ and 10/sup 5/ per cm/sup 3/. Real-time measurements of radon and progeny concentrations, particles and environmental parameters, were made throughout the experiments. Measurements of plated out activity on the surfaces of internal walls of the room and fan blades were measured directly by filter papers and CR-39 nuclear track detectors placed on the surfaces. The ratio of plated out activity to the total progeny activity in the room varied from 17% to 84%, in inverse relation to the particle concentrations. More than 99% of the plateout activity was found on walls and less than 1% on the fan blades. (author).

  15. Reduction of radon progeny concentration in ordinary room due to a mixing fan

    Energy Technology Data Exchange (ETDEWEB)

    Abu-Jarad, F. (University of Petroleum and Minerals, Dhahran (Saudi Arabia). Dept. of Mechanical Engineering); Sextro, R.G. (Lawrence Berkeley Lab., CA (USA))

    1988-01-01

    Three experiments were carried out at the Indoor Air Quality Research House (IAQRH) at Lawrence Berkeley Laboratory, University of California, to study the effect of operating a mixing fan on the radon progeny. In every experiment, about 15 KBq.m{sup -3} of radon was injected into a 36 m3 ordinary room. Measurements were conducted with three different aerosol particle concentrations of about 10{sup 2}, 10{sup 4} and 10{sup 5} per cm3. Real-time measurements of radon and progeny concentrations, particles, and environmental parameters were made throughout the experiments. Measurements of plate-out activity on the surfaces of internal walls of the room were measured directly by filter papers and CR-39 nuclear track detectors, while on fan blades CR-39 detectors were used. The ratio of plated-out activity to the total progeny activity in the room varied from 17% to 84%. More than 99% of the plated-out activity was found on walls and less than 1% on fan blades. (author).

  16. Inheritance of nitrogen use efficiency in inbred progenies of tropical maize based on multivariate diallel analysis.

    Science.gov (United States)

    Guedes, Fernando Lisboa; Diniz, Rafael Parreira; Balestre, Marcio; Ribeiro, Camila Bastos; Camargos, Renato Barbosa; Souza, João Cândido

    2014-01-01

    The objective of our study was to characterize and determine the patterns of genetic control in relation to tolerance and efficiency of nitrogen use by means of a complete diallel cross involving contrasting inbred progenies of tropical maize based on a univariate approach within the perspective of a multivariate mixed model. Eleven progenies, previously classified regarding the tolerance and responsiveness to nitrogen, were crossed in a complete diallel cross. Fifty-five hybrids were obtained. The hybrids and the progenies were evaluated at two different nitrogen levels, in two locations. The grain yield was measured as well as its yield components. The heritability values between the higher and lower nitrogen input environment did not differ among themselves. It was observed that the general combining ability values were similar for both approaches univariate and multivariate, when it was analyzed within each location and nitrogen level. The estimate of variance of the specific combining ability was higher than general combining ability estimate and the ratio between them was 0.54. The univariate and multivariate approaches are equivalent in experiments with good precision and high heritability. The nonadditive genetic effects exhibit greater quantities than the additive genetic effects for the genetic control of nitrogen use efficiency.

  17. Inheritance of Nitrogen Use Efficiency in Inbred Progenies of Tropical Maize Based on Multivariate Diallel Analysis

    Directory of Open Access Journals (Sweden)

    Fernando Lisboa Guedes

    2014-01-01

    Full Text Available The objective of our study was to characterize and determine the patterns of genetic control in relation to tolerance and efficiency of nitrogen use by means of a complete diallel cross involving contrasting inbred progenies of tropical maize based on a univariate approach within the perspective of a multivariate mixed model. Eleven progenies, previously classified regarding the tolerance and responsiveness to nitrogen, were crossed in a complete diallel cross. Fifty-five hybrids were obtained. The hybrids and the progenies were evaluated at two different nitrogen levels, in two locations. The grain yield was measured as well as its yield components. The heritability values between the higher and lower nitrogen input environment did not differ among themselves. It was observed that the general combining ability values were similar for both approaches univariate and multivariate, when it was analyzed within each location and nitrogen level. The estimate of variance of the specific combining ability was higher than general combining ability estimate and the ratio between them was 0.54. The univariate and multivariate approaches are equivalent in experiments with good precision and high heritability. The nonadditive genetic effects exhibit greater quantities than the additive genetic effects for the genetic control of nitrogen use efficiency.

  18. Postoperative recurrence in hepatic hydatid disease.

    Science.gov (United States)

    Kapan, Metin; Kapan, Selin; Goksoy, Ertugrul; Perek, Sadik; Kol, Ece

    2006-05-01

    Recurrence of hepatic hydatid disease is still a serious problem in endemic areas like our country. In this study, we present the causes and management of recurrences after surgical therapy of the hepatic hydatid cysts. Hepatic hydatid cyst patients treated surgically and followed afterward at Istanbul University, Cerrahpasa Medical Faculty, Department of General Surgery between January 1998 and January 2003 were evaluated retrospectively. During this period, 172 primary patients with hepatic hydatid disease were attended to at our clinic. Morbidity and mortality rates for this series were 5.8% and 0.58%, respectively. Recurrence rate was 4.65% during the follow-up period of 60.5 months (range, 25-84 months). Primary causes of recurrence were thought to be unnoticed cysts with exophytic development due to inadequate incision and exposition and spreading of the disease during conservative operative interventions. It is concluded that selection of the proper incision allowing complete exposition, and performance of pericystectomy in solitary, peripherally located cysts prevent recurrence.

  19. Particle size distribution of the radon progeny and ambient aerosols in the Underground Tourist Route "Liczyrzepa" Mine in Kowary Adit

    Science.gov (United States)

    Wołoszczuk, Katarzyna; Skubacz, Krystian

    2018-01-01

    Central Laboratory for Radiological Protection, in cooperation with Central Mining Institute performed measurements of radon concentration in air, potential alpha energy concentration (PAEC), particle size distribution of the radon progeny and ambient aerosols in the Underground Tourist-Educational Route "Liczyrzepa" Mine in Kowary Adit. A research study was developed to investigate the appropriate dose conversion factors for short-lived radon progeny. The particle size distribution of radon progeny was determined using Radon Progeny Particle Size Spectrometer (RPPSS). The device allows to receive the distribution of PAEC in the particle size range from 0.6 nm to 2494 nm, based on their activity measured on 8 stages composed of impaction plates or diffusion screens. The measurements of the ambient airborne particle size distribution were performed in the range from a few nanometres to about 20 micrometres using Aerodynamic Particle Sizer (APS) spectrometer and the Scanning Mobility Particle Sizer Spectrometer (SMPS).

  20. Epstein-Barr Virus BKRF4 Gene Product Is Required for Efficient Progeny Production.

    Science.gov (United States)

    Masud, H M Abdullah Al; Watanabe, Takahiro; Yoshida, Masahiro; Sato, Yoshitaka; Goshima, Fumi; Kimura, Hiroshi; Murata, Takayuki

    2017-09-13

    Epstein-Barr virus (EBV), a member of human gammaherpesvirus, infects mainly B cells. EBV has two alternative life cycles, latent and lytic, and is reactivated occasionally from the latent stage to the lytic cycle. To combat EBV-associated disorders, understanding the molecular mechanisms of the EBV lytic replication cycle is also important. Here, we focused on an EBV lytic gene, BKRF4. Using our anti-BKRF4 antibody, we revealed that the BKRF4 gene product is expressed during the lytic cycle with late kinetics. To characterize the role of BKRF4, we constructed BKRF4-knockout mutants using the bacterial artificial chromosome (BAC) and CRISPR/Cas9 systems. While disruption of the BKRF4 gene had almost no effect on viral protein expression and DNA synthesis, it significantly decreased progeny virion levels in HEK293 and Akata cells. Furthermore, we show that BKRF4 is involved not only in production of progeny virions but also in increasing the infectivity of the virus particles. Immunoprecipitation assays revealed that BKRF4 interacted with a virion protein, BGLF2. We showed that the C-terminal region of BKRF4 was critical for this interaction and for efficient progeny production. Immunofluorescence analysis revealed that BKRF4 partially colocalized with BGLF2 in the nucleus and perinuclear region. Finally, we showed that BKRF4 is a phosphorylated, possible tegument protein and that the EBV protein kinase BGLF4 may be important for this phosphorylation. Taken together, our data suggest that BKRF4 is involved in the production of infectious virions.IMPORTANCE While the latent genes of EBV have been studied extensively, the lytic genes are less well characterized. This study focused on one such lytic gene, BKRF4, which is conserved only among gammaherpesviruses (ORF45 of Kaposi's sarcoma-associated herpesvirus or murine herpesvirus-68). After preparing the BKRF4 knockout virus using B95-8 EBV-BAC, we demonstrated that the BKRF4 gene was involved in infectious progeny

  1. Coping with Fear of Recurrence

    Science.gov (United States)

    ... What Comes Next After Finishing Treatment Coping With Fear of Recurrence Having a Baby After Cancer: Pregnancy ... treatment and preparing for the future. Coping With Fear of Recurrence Learn ways to manage the fear ...

  2. Recurrent Aphthous Stomatitis: A Review

    OpenAIRE

    Edgar, Natalie Rose; Saleh, Dahlia; Miller, Richard A.

    2017-01-01

    Aphthous stomatitis is a painful and often recurrent inflammatory process of the oral mucosa that can appear secondary to various well-defined disease processes. Idiopathic recurrent aphthous stomatitis is referred to as recurrent aphthous stomatitis. The differential diagnosis for recurrent aphthous ulcerations is extensive and ranges from idiopathic benign causes to inherited fever syndromes, to connective tissue disease, or even inflammatory bowel diseases. A thorough history and review of...

  3. Eco-logo-genetical characteristic of seed progeny of the Taraxacum officinale S.L. from the zone of east-ural radioactive trace

    Energy Technology Data Exchange (ETDEWEB)

    Pozolotina, V.; Ulyanova, E. [Russian Academy of Sciences, Institute of Plant and Animal Ecology, Ural Division, Ekaterinburg (Russian Federation)

    2004-07-01

    Levels of soil contamination with {sup 90}Sr and {sup 137}Cs radionuclides within the zone of Eastern-Ural radioactive trace, at the plots differently distant from the place of Kyshtym accident of 1957, exceed values of the global level 4-40 times. It was shown that the same coeno-population from EURT in the different years revealed the whole variety of previously described low-level irradiation effects. They are: stimulating one (vitality indexes in seed progeny exceed significantly those registered in the background samples); the depressing one, marked for high mortality level; and an indifferent effect showing neither significant differences from the background plants. Variation scope exceeding the normal one in regard to plants growth and development rates, as well as high frequencies of chromosome and morphological aberrations indicate to the plant genome non-stability caused by chronic low-level irradiation loads. Seed progeny also demonstrated the similar ambiguous response to additional irradiation, which is confirmed by allozyme analysis results. Standard electrophoretic methods are applicable to the dandelion studies. Some enzyme systems (Got, Dia) seem to be more suitable for seedlings, whereas the others provided satisfactory results being used for the seed material. In plant coeno-populations situated in radionuclides-polluted zone, genomic recombination processes show higher intensity. High enzymatic variability provides the material for natural selection and increase the adaptive potential of coeno-populations. (author)

  4. Development of a new thoron progeny detector based on SSNTD and the collection by an electric field.

    Science.gov (United States)

    Bi, L; Tschiersch, J; Meisenberg, O; Wielunski, M; Li, J L; Shang, B

    2011-05-01

    The importance of (220)Rn (thoron) progeny for human exposure has been widely recognised in the past decades. Since no stable equilibrium factor was found between indoor thoron and its progeny, and the concentration of thoron progeny varies with time, it is necessary to develop detectors for long-term measurement that directly sample and detect thoron progeny. However, power supply of this kind of detectors has always been a problem. In this study, a set of device that is suitable for long-term measurement is introduced. A high-voltage electric field was formed for the collection of charged aerosols attached by (222)Rn (radon) and thoron progenies on solid-state nuclear track detector. Impact from radon progeny could be eliminated with a shield of Al foil of appropriate thickness. Tests were made both in an experimental house and in a thoron chamber in Helmholtz Zentrum München to determine the parameters and to verify the universality under different conditions.

  5. The effect of yield components on grain yield in different progeny types of an F3 maize population

    Directory of Open Access Journals (Sweden)

    Stojković Slaviša

    2009-01-01

    Full Text Available This paper deals with the investigation of S1 and HS progenies obtained from an F3 maize population. Those two progeny groups were created in 2004, and field trials were carried out in the period 2005-2006 at three locations near Aleksinac, Leskovac, and Kruševac, in RCB design. After genotypic correlation coefficients were calculated, they were used to calculate path coefficients and multiple determination coefficients. Analysis of path coefficients showed significant direct effects of the all studied traits in both S1 and HS progeny types. In S1 progenies indirect effects were significant for the all paths, except for percent of stalk and root lodged plants through 1000 grain mass, 1000 grain mass through percent of stalk and root lodged plants, and for 1000 grain mass through number of grain rows. The strongest direct effect on grain yield in HS progenies was shown by percent of stalk and root lodged plants (0.68**, while number of grains per row gave the strongest negative direct effect on grain yield (-0.97**. Multiple determination coefficients were significant for the most of independent variables' combinations in both progeny types. Numerous combinations composed of just few traits showed joint effects almost equal to the one showed by all seven independent variables.

  6. Variability of silver fir (Abies alba Mill. progeny from the Tisovik Reserve expressed in needle traits and chloroplast microsatellite DNA

    Directory of Open Access Journals (Sweden)

    Pawlaczyk Ewa M.

    2017-01-01

    Full Text Available Progeny from nineteen family lines of silver fir (Abies alba Mill. from the Tisovik Reserve growing in an experimental plot were analyzed based on 4 chloroplast microsatellite DNA loci and 12 morphological and anatomical needle traits. The Tisovik Reserve is located in Białowieża Primeval Forest, 120 km north of the natural range limit of this species, and embraces a small and isolated natural population of silver fir. The aim of this study was to determine genetic variation within and between progeny lines. Analysis of phenotypic variation showed that the traits which differed most among individuals were the needle width and the distance from resin canals to vascular bundle. Those traits, which differed most between the progeny lines, were the number of endodermic cells around the vascular bund and the weight of hypodermic cells. In Tisovik progeny, we detected 107 different haplotypes. In progeny lines, we detected more haplotypes than in maternal trees, and most haplotypes did not exist in maternal trees. This may be the result of pollen influx from other silver fir stands. Progeny from Tisovik showed a higher level of variability in comparison with maternal trees.

  7. Causes of recurrent pneumonia in children in a general hospital.

    Science.gov (United States)

    Hoving, M F Paulien; Brand, Paul L P

    2013-03-01

    Because the few previous studies on underlying causes of recurrent pneumonia in children have come from tertiary care referral centres where selection bias may be important, the aim of this study was to examine underlying causes of recurrent pneumonia in children in a general hospital. We performed a retrospective chart review in a general hospital of 62 children with recurrent pneumonia over a 7.5 years period. In 19 patients (30.6%), no cause was identified, commonly because favourable natural history obviated the need for a full and invasive diagnostic work-up. Other underlying causes included recurrent aspiration in 16 patients (25.7%), lung disease (airway stenosis, bronchiectasis, middle lobe syndrome or tracheooesophageal fistula) in 10 patients (16.1%) and immune deficiency in 10 patients (16.1%). In contrast to previous studies, asthma was never diagnosed as an underlying cause, but diagnostic confusion between asthma (or recurrent upper respiratory tract infections) and recurrent pneumonia was common. The cause of recurrent pneumonia in children remains elusive in almost a third of patients, partly because the favourable natural history consistent with immune system maturation eliminates the need for further diagnostic procedures. Asthma is more likely a differential diagnostic consideration than an underlying cause of recurrent pneumonia in children. A standardised diagnostic guideline is needed to improve knowledge on causes of recurrent pneumonia in children. © 2013 The Authors. Journal of Paediatrics and Child Health © 2013 Paediatrics and Child Health Division (Royal Australasian College of Physicians).

  8. Immunomodulators to treat recurrent miscarriage

    NARCIS (Netherlands)

    Prins, Jelmer R.; Kieffer, Tom E.C.; Scherjon, Sicco A.

    2014-01-01

    Recurrent miscarriage is a reproductive disorder affecting many couples. Although several factors are associated with recurrent miscarriage, in more than 50% of the cases the cause is unknown. Maladaptation of the maternal immune system is associated with recurrent miscarriage and could explain part

  9. Zinc distribution and speciation in Arabidopsis halleri x Arabidops is lyrata progenies presenting various zinc accumulation capacities

    Energy Technology Data Exchange (ETDEWEB)

    Sarret, Geraldine; Willems, Glenda; Isaure, Marie-Pierre; Marcus, Matthew A.; Fakra, Sirine C.; Frerot, Helene; Pairis, Sebastien; Geoffroy, Nicolas; Manceau, Alain; Saumitou-Laprade, Pierre

    2010-04-08

    - The purpose of this study was to investigate the relationship between the chemical form and localization of zinc (Zn) in plant leaves and their Zn accumulationcapacity. - An interspecific cross between Arabidopsis halleri sp. halleri and Arabidopsis lyrata sp. petrea segregating for Zn accumulation was used. Zinc (Zn) speciation and Zn distribution in the leaves of the parent plants and of selected F1 and F2 progenies were investigated by spectroscopic and microscopic techniques and chemical analyses. - A correlation was observed between the proportion of Zn being in octahedral coordination complexed to organic acids and free in solution (Zn?OAs + Znaq) and Zn content in the leaves. This pool varied between 40percent and 80percent of total leaf Zn depending on the plant studied. Elemental mapping of the leaves revealed different Zn partitioning between the veins and the leaf tissue. The vein : tissue fluorescence ratio was negatively correlated with Zn accumulation. - The higher proportion of Zn?OAs + Znaq and the depletion of the veins in the stronger accumulators are attributed to a higher xylem unloading and vacuolar sequestration in the leaf cells. Elemental distributions in the trichomes were also investigated, and results support the role of carboxyl and⁄ or hydroxyl groups as major Zn ligands in these cells.

  10. Application of LW7 marker for identification of progenies with male sterility gene in sweet sorghum population

    Directory of Open Access Journals (Sweden)

    Darika Bunphan

    2013-04-01

    Full Text Available The objectives of this study were to verify the use of LW7 marker in identifying maintainer lines (B-lines and restorer lines (R-lines in grain sorghum and sweet sorghum, and to identify B-lines in the F2, BC1F2 and BC2F2 generations. Twenty five accessions of sorghum were evaluated, and LW7 marker correctly identified accessions which presented male sterility gene (rf4 in Suphan Buri1 and 03B cultivars; moreover, these genotypes did not show 779 bp band. The cross between Suphan Buri1 and a male-sterile line (A-line 03A resulted in a sterilized male, confirming the usefulness of the marker in breeding programs. B-lines in the F2, BC1F2 and BC2F2 generations were identified by LW7 marker. The segregation ratio of 3:1 for male fertility and male sterility in the progenies of the three generations supported the one-gene model of Mendelian segregation. The use of marker assisted selection was successful for line development of sweet sorghum with male sterility

  11. Gross karyotypic and phenotypic alterations among different progenies of the Candida glabrata CBS138/ATCC2001 reference strain.

    Directory of Open Access Journals (Sweden)

    Oliver Bader

    Full Text Available Genomic plasticity is a mechanism for adaptation to environmental cues such as host responses and antifungal drug pressure in many fungi including the human pathogenic yeast Candida glabrata. In this study we evaluated the phenotypic and genotypic stability of the world-wide used C. glabrata reference strain CBS138/ATCC2001 under laboratory conditions. A set of ten lineages of this wild type strain and genetically modified progenies were obtained from different scientific laboratories, and analyzed for genotypic and phenotypic alterations. Even though the derivates were indistinguishable by multi locus sequence typing, different phenotypic groups that correlated with specific karyotypic changes were observed. In addition, modifications in the adherence capacity to plastic surface emerged that were shown to correlate with quantitative changes in adhesin gene expression rather than subtelomeric gene loss or differences in the number of macrosatellite repeats within adhesin genes. These results confirm the genomic plasticity of C. glabrata and show that chromosomal aberrations and functional adaptations may occur not only during infection and under antimicrobial therapy, but also under laboratory conditions without extreme selective pressures. These alterations can significantly affect phenotypic properties such as cell surface attributes including adhesion and the cell wall carbohydrate composition and therefore, if unnoticed, may adulterate the outcome of genetic studies.

  12. Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene

    Directory of Open Access Journals (Sweden)

    Brad Gilbertson

    2016-08-01

    Full Text Available The influenza A virus genome comprises eight negative-sense viral RNAs (vRNAs that form individual ribonucleoprotein (RNP complexes. In order to incorporate a complete set of each of these vRNAs, the virus uses a selective packaging mechanism that facilitates co-packaging of specific gene segments but whose molecular basis is still not fully understood. Recently, we used a competitive transfection model where plasmids encoding the A/Puerto Rico/8/34 (PR8 and A/Udorn/307/72 (Udorn PB1 gene segments were competed to show that the Udorn PB1 gene segment is preferentially co-packaged into progeny virions with the Udorn NA gene segment. Here we created chimeric PB1 genes combining both Udorn and PR8 PB1 sequences to further define the location within the Udorn PB1 gene that drives co-segregation of these genes and show that nucleotides 1776–2070 of the PB1 gene are crucial for preferential selection. In vitro assays examining specific interactions between Udorn NA vRNA and purified vRNAs transcribed from chimeric PB1 genes also supported the importance of this region in the PB1-NA interaction. Hence, this work identifies an association between viral genes that are co-selected during packaging. It also reveals a region potentially important in the RNP-RNP interactions within the supramolecular complex that is predicted to form prior to budding to allow one of each segment to be packaged in the viral progeny. Our study lays the foundation to understand the co-selection of specific genes, which may be critical to the emergence of new viruses with pandemic potential.

  13. Recurrent giant juvenile fibroadenoma

    Directory of Open Access Journals (Sweden)

    Kathryn S. King

    2017-11-01

    Full Text Available Breast masses in children, though rare, present a difficult clinical challenge as they can represent a wide variety of entities from benign fibroadenomas to phyllodes tumors. Rapidly growing or recurrent masses can be particularly concerning to patients, families and physicians alike. Clinical examination and conventional imaging modalities are not efficacious in distinguishing between different tumor types and surgical excision is often recommended for both final diagnosis and for treatment of large or rapidly growing masses. While surgical excision can result in significant long-term deformity of the breast there are some surgical techniques that can be used to limit deformity and/or aid in future reconstruction. Here we present a case of recurrent giant juvenile fibroadenoma with a review of the clinical presentation, diagnostic tools and treatment options.

  14. Recurrent Takotsubo Cardiomyopathy

    Directory of Open Access Journals (Sweden)

    Erkan Yıldırım

    2016-09-01

    Full Text Available Stress-induced cardiomyopathy or takotsubo syndrome is a transient cardiac dysfunction which is precipitated by intense emotional or physical stress. It mimics acute coronary syndrome clinically (chest pain and dyspnea and has specific echocardiographic findings in the absence of significant coronary stenosis. The underlying pathophysiologic mechanism is mostly due to stress-induced catecholamine release. This syndrome is more commonly seen in females especially in the postmenopausal period. The prognosis of stress-induced cardiomyopathy is usually favorable with a prevalence of in-hospital mortality of less than 2%. Reports of a single episode of stress-induced cardiomyopathy are common in the literature, but recurrence of stress-induced cardiomyopathy is rare. Herein, we present an uncommon case of recurrent stress-induced cardiomyopathy in order to provide an explanation of the pathophysiology of this unique syndrome.

  15. Recurrent amiodarone pulmonary toxicity.

    Science.gov (United States)

    Chendrasekhar, A; Barke, R A; Druck, P

    1996-01-01

    Amiodarone, a widely used antiarrhythmic drug, is associated with pulmonary toxicity, with an estimated mortality of 1% to 33%. Standard treatment for amiodarone pulmonary toxicity (APT) has been discontinuance of the drug and steroid therapy. We report a case of APT that recurred after withdrawal of steroids and failed to respond to reinstatement of steroid therapy. Recurrent APT is a rare clinical entity that has been reported only twice in recent literature.

  16. Recurrent aphthous stomatitis.

    Science.gov (United States)

    Akintoye, Sunday O; Greenberg, Martin S

    2005-01-01

    The cause of recurrent aphthous stomatitis (RAS) remains unknown despite considerable research. This article reviews the evidence for current theories regarding this disorder, including possible suspected relationships with microbial and immunologic factors, and presents medical diseases that mimic RAS lesions in certain patients. Topical management of the common form of minor RAS is described along with systemic therapy currently available to patients with severe forms of this disease.

  17. Modularity promotes epidemic recurrence

    CERN Document Server

    Jesan, T; Sinha, Sitabhra

    2016-01-01

    The long-term evolution of epidemic processes depends crucially on the structure of contact networks. As empirical evidence indicates that human populations exhibit strong community organization, we investigate here how such mesoscopic configurations affect the likelihood of epidemic recurrence. Through numerical simulations on real social networks and theoretical arguments using spectral methods, we demonstrate that highly contagious diseases that would have otherwise died out rapidly can persist indefinitely for an optimal range of modularity in contact networks.

  18. Recurrent cutaneous leishmaniasis

    OpenAIRE

    Gomes,Ciro Martins; Damasco,Fabiana dos Santos; Morais,Orlando Oliveira de; Paula,Carmen Dea Ribeiro de; Sampaio,Raimunda Nonata Ribeiro

    2013-01-01

    We present a case of an 18-year-old male patient who, after two years of inappropriate treatment for cutaneous leishmaniasis, began to show nodules arising at the edges of the former healing scar. He was immune competent and denied any trauma. The diagnosis of recurrent cutaneous leishmaniasis was made following positive culture of aspirate samples. The patient was treated with N-methylglucamine associated with pentoxifylline for 30 days. Similar cases require special attention mainly because...

  19. Multiplex Recurrence Networks

    Science.gov (United States)

    Eroglu, Deniz; Marwan, Norbert

    2017-04-01

    The complex nature of a variety of phenomena in physical, biological, or earth sciences is driven by a large number of degrees of freedom which are strongly interconnected. Although the evolution of such systems is described by multivariate time series (MTS), so far research mostly focuses on analyzing these components one by one. Recurrence based analyses are powerful methods to understand the underlying dynamics of a dynamical system and have been used for many successful applications including examples from earth science, economics, or chemical reactions. The backbone of these techniques is creating the phase space of the system. However, increasing the dimension of a system requires increasing the length of the time series in order get significant and reliable results. This requirement is one of the challenges in many disciplines, in particular in palaeoclimate, thus, it is not easy to create a phase space from measured MTS due to the limited number of available obervations (samples). To overcome this problem, we suggest to create recurrence networks from each component of the system and combine them into a multiplex network structure, the multiplex recurrence network (MRN). We test the MRN by using prototypical mathematical models and demonstrate its use by studying high-dimensional palaeoclimate dynamics derived from pollen data from the Bear Lake (Utah, US). By using the MRN, we can distinguish typical climate transition events, e.g., such between Marine Isotope Stages.

  20. Stress Hormones Epinephrine and Corticosterone Selectively Modulate Herpes Simplex Virus 1 (HSV-1) and HSV-2 Productive Infections in Adult Sympathetic, but Not Sensory, Neurons.

    Science.gov (United States)

    Ives, Angela M; Bertke, Andrea S

    2017-07-01

    Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) infect and establish latency in peripheral neurons, from which they can reactivate to cause recurrent disease throughout the life of the host. Stress is associated with the exacerbation of clinical symptoms and the induction of recurrences in humans and animal models. The viruses preferentially replicate and establish latency in different subtypes of sensory neurons, as well as in neurons of the autonomic nervous system that are highly responsive to stress hormones. To determine if stress-related hormones modulate productive HSV-1 and HSV-2 infections within sensory and autonomic neurons, we analyzed viral DNA and the production of viral progeny after treatment of primary adult murine neuronal cultures with the stress hormones epinephrine and corticosterone. Both sensory trigeminal ganglion (TG) and sympathetic superior cervical ganglion (SCG) neurons expressed adrenergic receptors (activated by epinephrine) and the glucocorticoid receptor (activated by corticosterone). Productive HSV infection colocalized with these receptors in SCG but not in TG neurons. In productively infected neuronal cultures, epinephrine treatment significantly increased the levels of HSV-1 DNA replication and production of viral progeny in SCG neurons, but no significant differences were found in TG neurons. In contrast, corticosterone significantly decreased the levels of HSV-2 DNA replication and production of viral progeny in SCG neurons but not in TG neurons. Thus, the stress-related hormones epinephrine and corticosterone selectively modulate acute HSV-1 and HSV-2 infections in autonomic, but not sensory, neurons. IMPORTANCE Stress exacerbates acute disease symptoms resulting from HSV-1 and HSV-2 infections and is associated with the appearance of recurrent skin lesions in millions of people. Although stress hormones are thought to impact HSV-1 and HSV-2 through immune system suppression, sensory and autonomic neurons that become

  1. Effect of indoor-generated airborne particles on radon progeny dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Trassierra, C. Vargas [Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, Cassino, FR (Italy); Stabile, L., E-mail: l.stabile@unicas.it [Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, Cassino, FR (Italy); Cardellini, F.; Morawska, L. [National Institute of Ionizing Radiation Metrology (INMRI-ENEA), Rome (Italy); Buonanno, G. [Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, Cassino, FR (Italy); International Laboratory for Air Quality and Health, Queensland University of Technology, Brisbane (Australia)

    2016-08-15

    Highlights: • Investigation of the interaction between particles and radon progeny dynamics. • Measurements of particles emitted by different indoor sources. • Tests performed in a controlled radon chamber. • Particle size strongly influences the radon progeny dynamics. • Particle surface area concentration is the key parameter of the radon-particle interaction. - Abstract: In order to investigate the interaction between radon progeny and particles, an experimental campaign was carried out in a radon chamber at the Italian National Institute of Ionizing Radiation Metrology, quantifying the amount of attached and unattached radon daughters present in air, as well as the equilibrium factor in the presence of particles generated through indoor sources. A fixed radon concentration was maintained, while particles were generated using incense sticks, mosquito coils and gas combustion. Aerosols were characterized in terms of particle concentrations and size distributions. Simultaneously, radon concentration and attached/unattached potential alpha energy concentration in the air were continuously monitored by two different devices, based on alpha spectroscopy techniques. The presence of particles was found to affect the attached fraction of radon decay products, in such a way that the particles acted as a sink for radionuclides. In terms of sources which emit large particles (e.g. incense, mosquito coils), which greatly increase particle surface area concentrations, the Equilibrium Factor was found to double with respect to the background level before particle generation sessions. On the contrary, the radon decay product dynamics were not influenced by gas combustion processes, mainly due to the small surface area of the particles emitted.

  2. Ongoing or re-emerging subjective insomnia symptoms after full/partial remission or recovery of major depressive disorder mainly with the selective serotonin reuptake inhibitors and risk of relapse or recurrence: a 52-week follow-up study.

    Science.gov (United States)

    Gulec, Mustafa; Selvi, Yavuz; Boysan, Murat; Aydin, Adem; Besiroglu, Lutfullah; Agargun, Mehmet Yucel

    2011-11-01

    Many patients who remit fully/partially or recover from an episode of major depression continue to suffer from sleep problems, mainly insomnia. Our study assesses the frequency and types of ongoing or re-emerging subjective insomnia symptoms and their relationship to subsequent depressive relapse or recurrence in a group of full/partial remitters or recoverers. Sixty patients, 18 to 65 years of age, with current MDD defined using DSM-IV-TR criteria were participated in the study. They began a 24-week course of open-label acute plus continuation phase treatment mainly with SSRIs. Maintenance treatment was not planned for not affecting natural relapse or recurrence rates. All participants were evaluated by CGI-S, BDI, HAMD-17, PSQI, and ISI at weeks 0, 4, 12, 24, and 52. Majority of the participants (83.33%) had subjective sleep disturbance, insomnia prior to relapse or recurrence. Repeated measures ANOVA was used to evaluate changes in scores of depression and sleep scales over time between recurred (N = 12) and non-recurred groups (N = 48). Differences between means were calculated with post hoc Bonferroni comparison test. The changes in scores of entire clinical scales over time between recurred and non-recurred groups were statistically significant. The limitations of this study include the use of a relatively small, mostly young female sample and the lack of an objective sleep measure to corroborate self-report scales. Early recognition and treatment of disturbances of the sleep-wake cycle may be important for treatment and prevention of recurrence of depression. Copyright © 2011 Elsevier B.V. All rights reserved.

  3. Dual-color Herpesvirus Capsids Discriminate Inoculum from Progeny and Reveal Axonal Transport Dynamics.

    Science.gov (United States)

    Scherer, Julian; Yaffe, Zachary A; Vershinin, Michael; Enquist, Lynn W

    2016-08-31

    Alpha herpesviruses, such as herpes simplex virus and pseudorabies virus (PRV), are neuroinvasive dsDNA viruses that establish life-long latency in peripheral nervous system (PNS) neurons of their native hosts. Following reactivation, the infection can spread back to the initial mucosal site of infection or, in rare cases, to the central nervous system with usually serious outcomes. During entry and egress, viral capsids depend on microtubule-based molecular motors for efficient and fast transport. In axons of PNS neurons, cytoplasmic dynein provides force for retrograde movements towards the soma, and kinesins move cargo in the opposite, anterograde direction. The dynamic properties of virus particles in cells can be imaged by fluorescent protein fusions to the small capsid protein VP26, which are incorporated into capsids. However, single-color fluorescent protein tags fail to distinguish virus inoculum from progeny. Therefore, we established a dual-color system by growing a recombinant PRV expressing a red fluorescent VP26 fusion (PRV180) on a stable cell line expressing a green VP26 fusion (PK15-mNG-VP26). The resulting dual-color virus preparation (PRV180G) contains capsids tagged with both red and green fluorescent proteins, and 97% of particles contain detectable levels of mNG-VP26. After replication in neuronal cells, all PRV180G progeny exclusively contain mRFP-VP26 tagged capsids. We used PRV180G for an analysis of axonal capsid transport dynamics in PNS neurons. Fast dual-color total internal reflection fluorescence (TIRF) microscopy, single particle tracking and motility analyses reveal robust, bidirectional capsid motility mediated by cytoplasmic dynein and kinesin during entry, whereas egressing progeny particles are exclusively transported by kinesins. Alpha herpesviruses are neuroinvasive viruses that infect the peripheral nervous system (PNS) of infected hosts as an integral part of their life cycle. Establishment of a quiescent or latent infection

  4. Development of a Progeny Marker for Steelhead; A Thesis submitted to Oregon State University.

    Energy Technology Data Exchange (ETDEWEB)

    Shippentower, Gene E. [Oregon State Univ., Corvallis, OR (United States)

    2009-04-15

    This study was undertaken to determine if strontium chloride could be used to create a trans-generational otolith mark in steelhead (Oncorhynchus mykiss). I completed two strontium injection trials and a survey of juvenile steelhead from various steelhead hatcheries. The two trials measured Sr:Ca ratios in otoliths in response to injections and the survey measured the natural variation in Sr:Ca ratios in otoliths of juvenile hatchery steelhead in response to the natural variation. In 2003, adult female Wallowa River, Oregon O. mykiss, were captured at the hatchery and evenly divided between a control group and two treatment groups. These females received an intraperitoneal injection of 1cc/500 g of body weight of a physiologically isotonic solution (0.9% saline) containing concentrations of 0 (control), 1000, or 5000 parts per million (ppm) of strontium chloride hexahydrate (SrCl2* 6H2O). Females were housed in a single outdoor tank until spawned artificially, and a distinct external tag identified each female within each treatment group. In 2004, female steelhead were captured throughout the duration of the adult returns to the Umatilla River basin and injected with 0, 1000, 5000, or 20,000-ppm strontium. In both trials, progeny of fish treated with strontium had significantly higher Sr:Ca ratios in the primordial region of their otoliths as measured using an electron wavelength dispersive microprobe. There was no difference in fertilization rates of eggs and survival rates of fry among treatment groups. Progeny from treated mothers were on average larger than progeny of untreated mothers. The Sr:Ca ratios in otoliths collected from various populations of steelhead were greater than the control values measured in both injections studies. This study suggests that the marking technique works and the utility for such a technique could be used for empirical observations in determining the relative fitness of progeny of adult hatchery origin fish

  5. Selection of Feed Intake or Feed Efficiency

    DEFF Research Database (Denmark)

    Veerkamp, Roel F; Pryce, Jennie E; Spurlock, Diane

    2013-01-01

    The widespread use of genomic information in dairy cattle breeding programs has opend up the possibility to select for novel traits, especially for traits that are traditionally difficult to record in a progeny testing scheme. Feed intake and efficiency is such a difficult to measure trait. In Fe...

  6. Multiscale recurrence quantification analysis of order recurrence plots

    Science.gov (United States)

    Xu, Mengjia; Shang, Pengjian; Lin, Aijing

    2017-03-01

    In this paper, we propose a new method of multiscale recurrence quantification analysis (MSRQA) to analyze the structure of order recurrence plots. The MSRQA is based on order patterns over a range of time scales. Compared with conventional recurrence quantification analysis (RQA), the MSRQA can show richer and more recognizable information on the local characteristics of diverse systems which successfully describes their recurrence properties. Both synthetic series and stock market indexes exhibit their properties of recurrence at large time scales that quite differ from those at a single time scale. Some systems present more accurate recurrence patterns under large time scales. It demonstrates that the new approach is effective for distinguishing three similar stock market systems and showing some inherent differences.

  7. Compression for preventing recurrence of venous ulcers.

    Science.gov (United States)

    Nelson, E Andrea; Bell-Syer, Sally E M

    2014-09-09

    moderate-compression hosiery (equivalent to UK class 2). The first study (n=300) found no significant reduction in recurrence at five years follow up with high-compression hosiery compared with moderate-compression (RR 0.82, 95% CI 0.61 to 1.12). The second study (n = 338) assessed ulcer recurrence at three years follow up and found that high-compression hosiery reduced recurrence compared with moderate-compression (RR 0.57, 95% CI 0.39 to 0.81). Statistically significant heterogeneity precluded meta-analysis of the results from these studies. Patient-reported compliance rates were reported in both trials;,there was significantly higher compliance with medium-compression than with high-compression hosiery in one and no significant difference in the second.A fourth trial (166 patients) found no statistically significant difference in recurrence between two types of medium (UK class 2) compression hosiery (Medi versus Scholl: RR 0.74, 95% CI 0.45 to 1.2).No trials of compression bandages for preventing ulcer recurrence were identified. There is evidence from one trial that compression hosiery reduces rates of reulceration of venous ulcers compared with no compression. Results from one trial suggest that recurrence is lower in high-compression hosiery than in medium-compression hosiery at three years whilst another trial found no difference at 5 years. Rates of patient intolerance of compression hosiery were high. There is insufficient evidence to aid selection of different types, brands, or lengths of compression hosiery.

  8. [Recurrent left atrial myxoma].

    Science.gov (United States)

    Moreno Martínez, Francisco L; Lagomasino Hidalgo, Alvaro; Mirabal Rodríguez, Roger; López Bermúdez, Félix H; López Bernal, Omaida J

    2003-01-01

    Primary cardiac tumors are rare. Mixomas are the most common among them; 75% are located in the left atrium, 20% in the right atrium, and the rest in the ventricles. The seldom appear in atrio-ventricular valves. Recidivant mixoma are also rare, appearing in 1-5% of all patients that have undergone surgical treatment of a mixoma. In this paper we present our experience with a female patient, who 8 years after having been operated of a left atrial mixoma, began with symptoms of mild heart failure. Transthoracic echocardiography revealed recurrence of the tumor, and was therefore subjected to a second open-heart surgery from which she recovered without complications.

  9. Recurrent Pregnancy Loss

    Directory of Open Access Journals (Sweden)

    Véronique Piroux

    1997-01-01

    Full Text Available Antiphospholipid antibodies (APA are associated with thrombosis, thrombocytopenia and fetal loss but they occur in a variety of diseases. Despite many efforts, a correlation between the specificity of particular subgroups of APA and particular clinical situations remains to be established. The antigens at the origin of APA remain to be identified. We discuss here the possible links between cell apoptosis or necrosis, leading to plasma membrane alterations, and the occurrence of APA in response to sustained stimulation. The pathogenic potential of APA is also considered with respect to recurrent pregnancy loss.

  10. Recurrent Miller Fisher syndrome.

    Science.gov (United States)

    Madhavan, S; Geetha; Bhargavan, P V

    2004-07-01

    Miller Fisher syndrome (MFS) is a variant of Guillan Barre syndrome characterized by the triad of ophthalmoplegia, ataxia and areflexia. Recurrences are exceptional with Miller Fisher syndrome. We are reporting a case with two episodes of MFS within two years. Initially he presented with partial ophthalmoplegia, ataxia. Second episode was characterized by full-blown presentation characterized by ataxia, areflexia and ophthalmoplegia. CSF analysis was typical during both episodes. Nerve conduction velocity study was fairly within normal limits. MRI of brain was within normal limits. He responded to symptomatic measures initially, then to steroids in the second episode. We are reporting the case due to its rarity.

  11. Impact of Co-Culturing with Fractionated Carbon-Ion-Irradiated Cancer Cells on Bystander Normal Cells and Their Progeny.

    Science.gov (United States)

    Autsavapromporn, Narongchai; Liu, Cuihua; Konishi, Teruaki

    2017-09-01

    The purpose of this study was to compare the biological effects of fractionated doses versus a single dose of high-LET carbon ions in bystander normal cells, and determine the effect on their progeny using the layered tissue co-culture system. Briefly, confluent human glioblastoma (T98G) cells received a single dose of 6 Gy or three daily doses of 2 Gy carbon ions, which were then seeded on top of an insert with bystander normal skin fibroblasts (NB1RGB) growing underneath. Cells were co-cultured for 6 h or allowed to grow for 20 population doublings, then harvested and assayed for different end points. A single dose of carbon ions resulted in less damage in bystander normal NB1RGB cells than the fractionated doses. In contrast, the progeny of bystander NB1RGB cells co-cultured with T98G cells exposed to fractionated doses showed less damage than progeny from bystander cells co-cultured with single dose glioblastoma cells. Furthermore, inhibition of gap junction communication demonstrated its involvement in the stressful effects in bystander cells and their progeny. These results indicate that dose fractionation reduced the late effect of carbon-ion exposure in the progeny of bystander cells compared to the effect in the initial bystander cells.

  12. Recurrent renal giant leiomyosarcoma.

    Science.gov (United States)

    Öziş, Salih Erpulat; Gülpınar, Kamil; Şahlı, Zafer; Konak, Baha Burak; Keskin, Mete; Özdemir, Süleyman; Ataoğlu, Ömür

    2016-01-01

    Primary renal leiomyosarcomas are rare, aggressive tumors. They constitute 1-2% of adult malignant renal tumors. Although leiomyosarcomas are the most common histological type (50-60%) of renal sarcomas, information on renal leiomyosarcoma is limited. Local or systemic recurrences are common. The radiological appearance of renal leiomyosarcomas is not specific, therefore renal leiomyosarcoma cannot be distinguished from renal cell carcinoma by imaging methods in all patients. A 74-year-old female patient presented to our clinic complaining of a palpable mass on the right side of her abdomen in November 2012. The abdominal magnetic resonance imaging revealed a mass, 25 × 24 × 23 cm in size. Her past medical history revealed that she has undergone right radical nephrectomy in 2007, due to a 11 × 12 × 13 cm renal mass that was then reported as renal cell carcinoma on abdominal magnetic resonance imaging, but the pathological diagnosis was low-grade renal leiomyosarcoma. The most recent follow-up of the patient was in 2011, with no signs of local recurrence or distant metastases within this four-year period. The patient underwent laparotomy on November 2012, and a 35 cm retroperitoneal mass was excised. The pathological examination of the mass was reported as high-grade leiomyosarcoma. The formation of this giant retroperitoneal mass in 1 year can be explained by the transformation of the lesion's pathology from low-grade to a high-grade tumor.

  13. Recurrent oral angioleiomyoma

    Directory of Open Access Journals (Sweden)

    V G Mahima

    2011-01-01

    Full Text Available Angioleiomyomas are vascular variant of leiomyomas which are benign tumors of smooth muscle. They are exceedingly rare in the oral cavity. Malignant transformation of these tumors has also been reported occasionally which warrants knowledge of this soft tissue tumor. A 57 year old male patient reported with a 15 day history of an asymptomatic growth that had started insidiously in his lower left back tooth region. Clinical examination revealed a solitary, oval, sessile growth in the mandibular left retro molar region. Excisional biopsy was suggestive of Angioleiomyoma. A recurrence of the same was noted two months later which was also histopathologically reported as Angioleiomyoma. The same was confirmed using special stains. This case reports an unusual presentation of Angioleiomyoma with regards to both recurrence as well as rapid growth. It is important to be well aware of this uncommon entity as these tumors often can mimic or transform into malignancy. Precise clinicopathological examinations are therefore invaluable in establishing an accurate diagnosis and delivering suitable treatment.

  14. Predicting expected progeny difference for marbling score in Angus cattle using artificial neural networks and Bayesian regression models.

    Science.gov (United States)

    Okut, Hayrettin; Wu, Xiao-Liao; Rosa, Guilherme J M; Bauck, Stewart; Woodward, Brent W; Schnabel, Robert D; Taylor, Jeremy F; Gianola, Daniel

    2013-09-11

    Artificial neural networks (ANN) mimic the function of the human brain and are capable of performing massively parallel computations for data processing and knowledge representation. ANN can capture nonlinear relationships between predictors and responses and can adaptively learn complex functional forms, in particular, for situations where conventional regression models are ineffective. In a previous study, ANN with Bayesian regularization outperformed a benchmark linear model when predicting milk yield in dairy cattle or grain yield of wheat. Although breeding values rely on the assumption of additive inheritance, the predictive capabilities of ANN are of interest from the perspective of their potential to increase the accuracy of prediction of molecular breeding values used for genomic selection. This motivated the present study, in which the aim was to investigate the accuracy of ANN when predicting the expected progeny difference (EPD) of marbling score in Angus cattle. Various ANN architectures were explored, which involved two training algorithms, two types of activation functions, and from 1 to 4 neurons in hidden layers. For comparison, BayesCπ models were used to select a subset of optimal markers (referred to as feature selection), under the assumption of additive inheritance, and then the marker effects were estimated using BayesCπ with π set equal to zero. This procedure is referred to as BayesCpC and was implemented on a high-throughput computing cluster. The ANN with Bayesian regularization method performed equally well for prediction of EPD as BayesCpC, based on prediction accuracy and sum of squared errors. With the 3K-SNP panel, for example, prediction accuracy was 0.776 using BayesCpC, and ranged from 0.776 to 0.807 using BRANN. With the selected 700-SNP panel, prediction accuracy was 0.863 for BayesCpC and ranged from 0.842 to 0.858 for BRANN. However, prediction accuracy for the ANN with scaled conjugate gradient back-propagation was lower

  15. Stability analysis of oil yield in oil palm (Elaeis guineensis) progenies in different environments.

    Science.gov (United States)

    Rafii, M Y; Jalani, B S; Rajanaidu, N; Kushairi, A; Puteh, A; Latif, M A

    2012-10-04

    We evaluated 38 dura x pisifera (DP) oil palm progenies in four locations in Malaysia for genotype by environment interaction and genotypic stability studies. The DP progenies derived from crosses between pisifera palms of AVROS, Serdang S27B, Serdang 29/36, and Lever Cameroon were chosen to be the males' parent and Deli dura palms designated as females' parent. All the locations differed in terms of soil physical and chemical properties, and the soil types ranged from coastal clay to inland soils. The genotype by environment interaction and stability of the individual genotypes were analyzed for oil yield trait using several stability techniques. A genotype by environment interaction was detected for oil yield and it had a larger variance component than genotypic variance (σ(2)(gl)/σ(2)(g) = 139.7%). Genotype by environment interaction of oil yield was largely explained by a non-linear relationship between genotypic and environmental values. Overall assessment of individual genotypic stability showed that seven genotypes were highly stable and had consistent performance over the environments for the oil yield trait [total individual genotype stability scored more than 10 and mean oil yielded above the average of the environment (genotype means are more than 34.37 kg·palm(-1)·year(-1))]. These genotypes will be useful for oil palm breeding and tissue culture programs for developing high oil yielding planting materials with stable performance.

  16. Inheritance of fruit colour in normal and irradiated progenies of brinjal

    Energy Technology Data Exchange (ETDEWEB)

    Gopimony, R.; George, M.K.; Gopinathan Nair, V. (College of Agriculture, Vellayani, Kerala (India))

    1980-12-01

    The inheritance of fruit colour in brinjal (Solanum melongena) was studied by analysing the progeny belonging to the F/sub 1/M/sub 2/, F/sub 1/M/sub 1/ and F/sub 2/M/sub 2/ generations, resulting from a cross between the varieties insanum and purple giant, followed by gamma irradiation. The F/sub 2/ phenotypic frequencies fitted very well with the dihybrid ratio indicating that the fruit colour is governed by two independently inherited genes. Three colour mutants, namely, purple, mottled green and white were induced in the F/sub 1/M/sub 1/ generation by the irradiation. The appearance of these mutants is explained due to independent mutations at either or both of the two goenetic loci. The colour pattern in the F/sub 2/M/sub 2/ progenies derived from the F/sub 1/M/sub 1/ mutants substantiates the two gene mechanism for the inheritance of fruit colour. The genotypes for the different colour types in the F/sub 1/, F/sub 2/ and F/sub 1/M/sub 1/ mutants have been indicated and discussed.

  17. Sex determination in beetles: Production of all male progeny by Parental RNAi knockdown of transformer

    Science.gov (United States)

    Shukla, Jayendra Nath; Palli, Subba Reddy

    2012-01-01

    Sex in insects is determined by a cascade of regulators ultimately controlling sex-specific splicing of a transcription factor, Doublesex (Dsx). We recently identified homolog of dsx in the red flour beetle, Tribolium castaneum (Tcdsx). Here, we report on the identification and characterization of a regulator of Tcdsx splicing in T. castaneum. Two male-specific and one female-specific isoforms of T. castaneum transformer (Tctra) were identified. RNA interference-aided knockdown of Tctra in pupa or adults caused a change in sex from females to males by diverting the splicing of Tcdsx pre-mRNA to male-specific isoform. All the pupa and adults developed from Tctra dsRNA injected final instar larvae showed male-specific sexually dimorphic structures. Tctra parental RNAi caused an elimination of females from the progeny resulting in production of all male progeny. Transformer parental RNAi could be used to produce all male population for use in pest control though sterile male release methods. PMID:22924109

  18. Saturated very long chain fatty acids are required for the production of infectious human cytomegalovirus progeny.

    Directory of Open Access Journals (Sweden)

    Emre Koyuncu

    Full Text Available Human cytomegalovirus hijacks host cell metabolism, increasing the flux of carbon from glucose to malonyl-CoA, the committed precursor to fatty acid synthesis and elongation. Inhibition of acetyl-CoA carboxylase blocks the production of progeny virus. To probe further the role of fatty acid metabolism during infection, we performed an siRNA screen to identify host cell metabolic enzymes needed for the production of infectious cytomegalovirus progeny. The screen predicted that multiple long chain acyl-CoA synthetases and fatty acid elongases are needed during infection, and the levels of RNAs encoding several of these enzymes were upregulated by the virus. Roles for acyl-CoA synthetases and elongases during infection were confirmed by using small molecule antagonists. Consistent with a role for these enzymes, mass spectrometry-based fatty acid analysis with ¹³C-labeling revealed that malonyl-CoA is consumed by elongases to produce very long chain fatty acids, generating an approximately 8-fold increase in C26-C34 fatty acid tails in infected cells. The virion envelope was yet further enriched in C26-C34 saturated fatty acids, and elongase inhibitors caused the production of virions with lower levels of these fatty acids and markedly reduced infectivity. These results reveal a dependence of cytomegalovirus on very long chain fatty acid metabolism.

  19. Endoscopic cubital tunnel recurrence rates.

    Science.gov (United States)

    Cobb, Tyson K; Sterbank, Patrick T; Lemke, Jon H

    2010-06-01

    Cubital tunnel syndrome is the second most common nerve entrapment in the upper extremity. There are no current publications concerning the recurrence rates after endoscopic cubital tunnel release. The purpose of this study is to evaluate the recurrence rate of endoscopic cubital tunnel release compared to published reports of recurrence following open cubital tunnel procedures. We reviewed 134 consecutive cases of endoscopic cubital tunnel release in 117 patients. There were 104 cases in 94 patients with greater than 3 months follow-up. The mean follow-up time was 736 days. They were grouped using Dellon's classification. Two literature control groups were used from published reports of recurrence rate following open cubital tunnel release. A recurrence was identified if the patient was symptom-free following surgery but had symptoms reappear 3 months or more after surgery as defined in the literature. Of the 104 cases, 92.31% had more than a 4-month follow-up. One case (0.96%) met the criteria for recurrence at 4 months postprocedure. Data were then compared to the literature control groups used from published reports of recurrence rates following open cubital tunnel release. Pooled, the combined controls had 22 of 180 cases (12.22%) with recurrences. The percentage of procedure recurrence varied significantly with p value equal to 0.0004. It is recognized that there is a lack of common classification and comparative analysis of these studies, but they do classify preoperative grading and recurrence similarly. We are 95% confident that our true recurrence rate is between 0.02% and 5.24% and that endoscopic cubital tunnel release has a recurrence rate, which is not higher than open cubital tunnel release literature controls.

  20. Radiation dose due to radon and thoron progeny inhalation in high-level natural radiation areas of Kerala, India.

    Science.gov (United States)

    Omori, Yasutaka; Tokonami, Shinji; Sahoo, Sarata Kumar; Ishikawa, Tetsuo; Sorimachi, Atsuyuki; Hosoda, Masahiro; Kudo, Hiromi; Pornnumpa, Chanis; Nair, Raghu Ram K; Jayalekshmi, Padmavaty Amma; Sebastian, Paul; Akiba, Suminori

    2017-03-20

    In order to evaluate internal exposure to radon and thoron, concentrations for radon, thoron, and thoron progeny were measured for 259 dwellings located in high background radiation areas (HBRAs, outdoor external dose: 3-5 mGy y(-1)) and low background radiation areas (control areas, outdoor external dose: 1 mGy y(-1)) in Karunagappally Taluk, Kerala, India. The measurements were conducted using passive-type radon-thoron detectors and thoron progeny detectors over two six-month measurement periods from June 2010 to June 2011. The results showed no major differences in radon and thoron progeny concentrations between the HBRAs and the control areas. The geometric mean of the annual effective dose due to radon and thoron was calculated as 0.10 and 0.44 mSv, respectively. The doses were small, but not negligible compared with the external dose in the two areas.

  1. Avaliaçao da produçao de resina em progenies de Pinus elliottii em idade juvenil

    OpenAIRE

    Oliveira, Jason Gonçalves de

    2013-01-01

    Os objetivos deste trabalho foram: estudar o potencial de produção de resina em progenies de Pinus elliottii na idade juvenil, correlacionar a produção de resina das progenies com parâmetros dendrométricos, estabelecer o número de avaliações necessárias para a melhor estimativa do potencial produtivo das progenies, comparar as variações da produção de resina com as condições climáticas, estimar os parâmetros genéticos relativos a produção de resina e, determinar a melhor metodologia e materia...

  2. Competition and its consequences for selection in barley breeding

    NARCIS (Netherlands)

    Spitters, C.J.T.

    1979-01-01

    The influence of competition is discussed and quantified for unselected bulk propagation, single-plant selection and yield testing of progenies in row plots. A mathematical model is introduced that defines the influence of intergenotypic competition and density of stand on the response to selection.

  3. Costs of selection schemes in small cattle populations

    Directory of Open Access Journals (Sweden)

    G. Gandini

    2010-04-01

    Full Text Available The objective of this study was to compare selection costs in cattle populations of 1,000 to 15,000 females, with the constraint of 1% of inbreeding per generation. Three selection schemes were analysed: no progeny test, with selection of sires on pedigree index (NPT; progeny test of sires of sires (PT3; classical progeny test of sires of sires and sires of dams (PT4. With the progeny test schemes, bulls were either culled after semen collection for progeny testing, or kept alive. Costs per year increased linearly with population size and showed divergent trends among strategies. PT4 was the most expensive scheme, ranging from 125,700 Euro (1,000 females to 318,900 Euro (15,000 females with the alive bull strategy, and from 20,200 Euro to 206,300 Euro with the cull bull strategy. NPT was generally cheaper than PT3 when bulls were culled. Costs per female and per semen dose were also analysed.

  4. Recurrent pregnancy loss

    DEFF Research Database (Denmark)

    Egerup, Pia; Kolte, A M; Larsen, E C

    2016-01-01

    STUDY QUESTION: Is there a different prognostic impact for consecutive and non-consecutive early pregnancy losses in women with secondary recurrent pregnancy loss (RPL)? SUMMARY ANSWER: Only consecutive early pregnancy losses after the last birth have a statistically significant negative prognostic...... impact in women with secondary RPL. WHAT IS KNOWN ALREADY: The risk of a new pregnancy loss increases with the number of previous pregnancy losses in patients with RPL. Second trimester losses seem to exhibit a stronger negative impact than early losses. It is unknown whether the sequence of pregnancy...... losses plays a role for the prognosis in patients with a prior birth. STUDY DESIGN, SIZE, DURATION: This retrospective cohort study of pregnancy outcome in patients with unexplained secondary RPL included in three previously published, Danish double-blinded placebo-controlled trials of intravenous...

  5. Analysis of Brain Recurrence

    Science.gov (United States)

    Frilot, Clifton; Kim, Paul Y.; Carrubba, Simona; McCarty, David E.; Chesson, Andrew L.; Marino, Andrew A.

    Analysis of Brain Recurrence (ABR) is a method for extracting physiologically significant information from the electroencephalogram (EEG), a non-stationary electrical output of the brain, the ultimate complex dynamical system. ABR permits quantification of temporal patterns in the EEG produced by the non-autonomous differential laws that govern brain metabolism. In the context of appropriate experimental and statistical designs, ABR is ideally suited to the task of interpreting the EEG. Present applications of ABR include discovery of a human magnetic sense, increased mechanistic understanding of neuronal membrane processes, diagnosis of degenerative neurological disease, detection of changes in brain metabolism caused by weak environmental electromagnetic fields, objective characterization of the quality of human sleep, and evaluation of sleep disorders. ABR has important beneficial implications for the development of clinical and experimental neuroscience.

  6. Effects of aposymbiotic and symbiotic aphids on parasitoid progeny development and adult oviposition behavior within aphid instars.

    Science.gov (United States)

    Cheng, Rui-Xia; Meng, Ling; Li, Bao-Ping

    2010-04-01

    This study aims at exploring the potential relationship between aphidiine parasitoid development and the primary endosymbiont in aphids by focusing on specific aphid instars and the relative effects on parasitoid oviposition behavior and progeny development. Lysiphlebus ambiguus (Aphidiidae, Hymenoptera) is a solitary parasitoid of several species of aphids, including Aphis fabae. In this study, A. fabae was treated with antibiotic rifampicin to obtain aposymbiotic hosts and exposed to parasitism. L. ambiguus launched significantly more attacks on symbiotic L(2) (the second instar), aposymbiotic L(3) (the third instar) and L(4) (the forth instar) hosts than on the corresponding hosts at the same age. L. ambiguus also parasitized more L(1) aphids compared with adults irrespective of whether the aphid was asymbiotic or not. Pupa mortality rate of parasitoid progeny was significantly lower from aposymbiotic hosts than from the corresponding symbiotics at all stages. Female-biased parasitoid progeny was produced from aposymbiotic aphids without respect to host ages, but female progeny increased linearly with host ages at parasitism from symbiotic aphids. Body size of parasitoid progeny increased linearly with host instars at parasitism in symbiotic aphids but did not significantly change across host instars in aposymbiotic aphids. The offspring parasitoids turned out to be generally large in body size from attacking aposymbiotic aphids compared with the symbiotics. Development time of egg-to-adult of parasitoid progeny decreased with host instars in both symbiotic and aposymbiotic aphids but was generally much longer in aposymbiotic aphids than in symbiotic aphids. Our study suggests that age or body size of host aphids may not be the only cue exercised by L. ambiguus to evaluate host quality and that offspring parasitoids may be able to compensate for the nutrition stress associated with disruption of primary endosymbiotc bacteria in aposymbiotic aphids.

  7. REPRODUCTIVE SYSTEM AND POLLEN FLOW IN PROGENIES OF Qualea grandiflora Mart., A TYPICAL SPECIES OF THE BRAZILIAN CERRADO

    Directory of Open Access Journals (Sweden)

    Lia Maris Orth Ritter Antiqueira

    2015-04-01

    Full Text Available This study analyzed the reproductive system and the pollen dispersion pattern of Qualea grandiflora progenies. This is a typical species from the Brazilian Cerrado about which there are not too many studies from the genetics point of view. The study was conducted in an area of 2.2 hectares located in the Conservation Unit managed by the Forest Institute of the state of São Paulo, Brazil (Assis State Forest. Total genomic DNA of 300 seeds from 25 plants (12 seeds from each plant was extracted and amplified using specific primers to obtain microsatellite markers. Results showed that selfing is frequent among adults and progenies, and the species reproduces by outcrossing between related and unrelated individuals (0.913. The single-locus outcrossing rate was 0.632, which indicates that mating between unrelated individuals is more frequent than between related plants. The selfing rate was low (0.087, that is, the species is allogamous and self-fertilization is reduced. About 35% of the plants in the progenies were full-sibs, and about 57%, half-sibs. Besides, about 8% of the progenies were selfing siblings. The genetic differentiation coefficient within progenies was 0.139, whereas the fixation rate was about 27%. The estimate of the effective size revealed that the genetic representativeness of descent was lower than expected in random mating progenies: The analyzed samples corresponded to only 13.2 individuals of an ideal panmictic population. In environmental recovery programs, seeds, preferably from different fruits, should be collected from 95 trees to preserve the genetic diversity of the species.

  8. Marker-Assisted Selection to Pyramid Nematode Resistance and the High Oleic Trait in Peanut

    Directory of Open Access Journals (Sweden)

    Y. Chu

    2011-07-01

    Full Text Available The dynamic challenges of peanut ( L. farming demand a quick response from breeders to develop new cultivars, a process that can be aided by the application of molecular markers. With the goal to pyramid nematode resistance and the trait for high oleic:linoleic acid (high O:L ratio in seeds, nematode-resistant cultivar Tifguard was used as the recurrent female parent and high O:L cultivars Georgia-02C and Florida-07 were used as donor parents for the high O:L trait. ‘Tifguard High O/L’ was generated through three rounds of accelerated backcrossing using BCF progenies selected with molecular markers for these two traits as the pollen donors. Selfed BCF plants yielded marker-homozygous individuals identified as Tifguard High O/L, compressing the hybridization and selection phases of the cultivar development process to less than 3 yr. The accuracy of marker-assisted selection (MAS was confirmed by phenotyping a subset of F populations from both parental combinations. Once additional molecular markers linked with traits of interest are designed to be compatible with high-throughput screening platforms, MAS will be more widely integrated into peanut breeding programs.

  9. Selecting an appropriate genetic evaluation model for selection in a developing dairy sector

    NARCIS (Netherlands)

    McGill, D.M.; Mulder, H.A.; Thomson, P.C.; Lievaart, J.J.

    2014-01-01

    This study aimed to identify genetic evaluation models (GEM) to accurately select cattle for milk production when only limited data are available. It is based on a data set from the Pakistani Sahiwal progeny testing programme which includes records from five government herds, each consisting of 100

  10. Floral-dip transformation of flax (Linum usitatissimum) to generate transgenic progenies with a high transformation rate.

    Science.gov (United States)

    Bastaki, Nasmah K; Cullis, Christopher A

    2014-12-19

    Agrobacterium-mediated plant transformation via floral-dip is a widely used technique in the field of plant transformation and has been reported to be successful for many plant species. However, flax (Linum usitatissimum) transformation by floral-dip has not been reported. The goal of this protocol is to establish that Agrobacterium and the floral-dip method can be used to generate transgenic flax. We show that this technique is simple, inexpensive, efficient, and more importantly, gives a higher transformation rate than the current available methods of flax transformation. In summary, inflorescences of flax were dipped in a solution of Agrobacterium carrying a binary vector plasmid (T-DNA fragment plus the Linum Insertion Sequence, LIS-1) for 1 - 2 min. The plants were laid flat on their side for 24 hr. Then, plants were maintained under normal growth conditions until the next treatment. The process of dipping was repeated 2 - 3 times, with approximately 10 - 14 day intervals between dipping. The T1 seeds were collected and germinated on soil. After approximately two weeks, treated progenies were tested by direct PCR; 2 - 3 leaves were used per plant plus the appropriate T-DNA primers. Positive transformants were selected and grown to maturity. The transformation rate was unexpectedly high, with 50 - 60% of the seeds from treated plants being positive transformants. This is a higher transformation rate than those reported for Arabidopsis thaliana and other plant species, using floral-dip transformation. It is also the highest, which has been reported so far, for flax transformation using other methods for transformation.

  11. Exposure of volunteers to a radon progeny enriched atmosphere; Exposition von freiwilligen Probanden in einer an Radonzerfallsprodukten angereicherten Atmosphaere

    Energy Technology Data Exchange (ETDEWEB)

    Butterweck-Dempewolf, G.; Schuler, C.; Vezzu, G. [Paul Scherrer Inst. (PSI), Villigen (Switzerland)

    1998-12-31

    For an improvement of dose estimation of the exposure by inhalation of radon progeny in the domestic environment human volunteers were exposed to precisely determined radon progeny atomospheres in the walk-in radon chamber at the Paul Scherrer Institut (PSI). One of the major targets of the experiments consists of the determination of the transport rate of the short-lived radon progeny from the respiratory tract into the blood circuit. The question is, if this transport may be fast enough compared to the physical half-life of the radon progeny to have a significant influence on dose considerations. The experimental set-up consisting of measurement devices for the size distribution of the unattached radon progeny during the exposure and for the activity of the radon progeny in the blood circuit is described. First volunteers have been exposed to atomspheres with a large fraction of unattached radon progeny. From these measurements, a larger transfer rate of the unattached radon progeny from respiratory tract to the blood circuit than reported in the literature can be derived, but without reaching values significantly influencing dose estimations. (orig.) [Deutsch] Mit dem Ziel, die Inhalationsdosis der Radonzerfallsprodukte im haeuslichen Umfeld besser abschaetzen zu koennen, wurden freiwillige Probanden in der begehbaren Radonkammer des Paul Scherrer Instituts (PSI) einer sehr genau bekannten Radonzerfallsproduktsatmosphaere ausgesetzt. Eines der Hauptziele dieser Experimente betrifft den Transport der kurzlebigen Radonzerfallsprodukte vom Atmungstrakt in den Blutkreislauf. Beantwortet werden soll die Frage, ob dieser Transport verglichen mit der physikalischen Halbwertzeit dieser Nuklide schnell genug sein koennte, um einen dosisrelevanten Einfluss zu haben. Der experimentelle Aufbau, bestehend aus Apparaturen fuer die Messung der Groessenverteilung des unangelagerten Anteils der Radonzerfallsprodukte waehrend der Expositionen und der Aktivitaet im Blutkreislauf

  12. Maternal-engineered nanomaterial exposure disrupts progeny cardiac function and bioenergetics.

    Science.gov (United States)

    Hathaway, Quincy A; Nichols, Cody E; Shepherd, Danielle L; Stapleton, Phoebe A; McLaughlin, Sarah L; Stricker, Janelle C; Rellick, Stephanie L; Pinti, Mark V; Abukabda, Alaeddin B; McBride, Carroll R; Yi, Jinghai; Stine, Seth M; Nurkiewicz, Timothy R; Hollander, John M

    2017-03-01

    Nanomaterial production is expanding as new industrial and consumer applications are introduced. Nevertheless, the impacts of exposure to these compounds are not fully realized. The present study was designed to determine whether gestational nano-sized titanium dioxide exposure impacts cardiac and metabolic function of developing progeny. Pregnant Sprague-Dawley rats were exposed to nano-aerosols (~10 mg/m 3 , 130- to 150-nm count median aerodynamic diameter) for 7-8 nonconsecutive days, beginning at gestational day 5-6 Physiological and bioenergetic effects on heart function and cardiomyocytes across three time points, fetal (gestational day 20 ), neonatal (4-10 days), and young adult (6-12 wk), were evaluated. Functional analysis utilizing echocardiography, speckle-tracking based strain, and cardiomyocyte contractility, coupled with mitochondrial energetics, revealed effects of nano-exposure. Maternal exposed progeny demonstrated a decrease in E- and A-wave velocities, with a 15% higher E-to-A ratio than controls. Myocytes isolated from exposed animals exhibited ~30% decrease in total contractility, departure velocity, and area of contraction. Bioenergetic analysis revealed a significant increase in proton leak across all ages, accompanied by decreases in metabolic function, including basal respiration, maximal respiration, and spare capacity. Finally, electron transport chain complex I and IV activities were negatively impacted in the exposed group, which may be linked to a metabolic shift. Molecular data suggest that an increase in fatty acid metabolism, uncoupling, and cellular stress proteins may be associated with functional deficits of the heart. In conclusion, gestational nano-exposure significantly impairs the functional capabilities of the heart through cardiomyocyte impairment, which is associated with mitochondrial dysfunction. NEW & NOTEWORTHY Cardiac function is evaluated, for the first time, in progeny following maternal nanomaterial inhalation. The

  13. The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity.

    Directory of Open Access Journals (Sweden)

    Mine Mumcuoglu

    Full Text Available BACKGROUND: Breast cancer is a remarkably heterogeneous disease. Luminal, basal-like, "normal-like", and ERBB2+ subgroups were identified and were shown to have different prognoses. The mechanisms underlying this heterogeneity are poorly understood. In our study, we explored the role of cellular differentiation and senescence as a potential cause of heterogeneity. METHODOLOGY/PRINCIPAL FINDINGS: A panel of breast cancer cell lines, isogenic clones, and breast tumors were used. Based on their ability to generate senescent progeny under low-density clonogenic conditions, we classified breast cancer cell lines as senescent cell progenitor (SCP and immortal cell progenitor (ICP subtypes. All SCP cell lines expressed estrogen receptor (ER. Loss of ER expression combined with the accumulation of p21(Cip1 correlated with senescence in these cell lines. p21(Cip1 knockdown, estrogen-mediated ER activation or ectopic ER overexpression protected cells against senescence. In contrast, tamoxifen triggered a robust senescence response. As ER expression has been linked to luminal differentiation, we compared the differentiation status of SCP and ICP cell lines using stem/progenitor, luminal, and myoepithelial markers. The SCP cells produced CD24+ or ER+ luminal-like and ASMA+ myoepithelial-like progeny, in addition to CD44+ stem/progenitor-like cells. In contrast, ICP cell lines acted as differentiation-defective stem/progenitor cells. Some ICP cell lines generated only CD44+/CD24-/ER-/ASMA- progenitor/stem-like cells, and others also produced CD24+/ER- luminal-like, but not ASMA+ myoepithelial-like cells. Furthermore, gene expression profiles clustered SCP cell lines with luminal A and "normal-like" tumors, and ICP cell lines with luminal B and basal-like tumors. The ICP cells displayed higher tumorigenicity in immunodeficient mice. CONCLUSIONS/SIGNIFICANCE: Luminal A and "normal-like" breast cancer cell lines were able to generate luminal-like and

  14. Norway spruce (Picea abies Karst. variability in progeny tests in Bosnia and Herzegovina

    Directory of Open Access Journals (Sweden)

    Cvjetković Branislav

    2017-01-01

    Full Text Available Norway spruce is one of the most important economic species is Bosnia and Herzegovina. It is the species at the edge of its natural range; nevertheless it attains significant growth and yield results. The species is often used for afforestation purposes. In the previous period, 4 progeny tests of Norway spruce were established in Bosnia and Herzegovina. The offspring from 6 natural populations: Han Pijesak 1, Han Pijesak 2, Foča, Potoci, Olovo and Kneževo were build-in the progeny tests. In 2016, the samples of Norway spruce from 2 progeny tests: Srebrenica and Drinić were collected. In total, 360 samples were collected. DNA isolation was done according to Dumoline et al. (1990. For assessment of genetic differences among populations, co-dominant nSSR microsatellite system had been used. The number of effective alleles ranged from 7.78 in the population Potoci up to 15 in the population Kneževo, the average number of alleles was 13. The observed heterozygosity ranged from 0.61 for the population Han Pijesak 2 up to 0.68 for population Kneževo. The average observed heterozygosity was 0.65. Fixation index was in the range from -0.073 in the population Potoci, to 0.030 for the population Han Pijesak 2. The average value of Wright fixation index is -0.007. The average fixation index indicates the existence of a very small number of homozygotes. Concerning the variability among populations it has been concluded that the total level of genetic differentiation among populations was very low (FST= 0.026. The result of Nei’s genetic distance shows that the populations Olovo and Potoci are separated from other populations. The results obtained by genetic markers, in addition with other, morphological and physiological markers will be the basis for the further investigation of Norway spruce adaptability and possibility for the transfer of genetic material in light of climate changes.

  15. Maternal aging affects life performance of progeny in a Holstein dairy cow model.

    Science.gov (United States)

    Astiz, S; Gonzalez-Bulnes, A; Sebastian, F; Fargas, O; Cano, I; Cuesta, P

    2014-10-01

    The development and life performance of 404 high-producing Holstein dairy cows was studied from birth onwards and during two lactations. The management, environment and parental genetics of the cows were known in detail. Cluster analysis identified four performance 'types': high-yielding (HY) cows and persistently high-yielding (PHY) cows, which accounted for 33% of the animals; medium-yielding (MY) cows, 41%; and low-yielding (LY) cows, 26%. Prenatal determinants of the life performance of the progeny were analyzed. Developmental and environmental factors were excluded as determinants of performance (including birth weight, level of passive immunity transfer, growth rate, age at first parturition and reproductive efficiency). Life performance did show minor seasonal effects, with more HY cows but less PHY being born during the cold season (90.1% in HY; 58.3% in PHY v. 81.5%). Instead, the single most important factor influencing life performance of daughters was maternal age. HY cows were born from the youngest mothers (1.89±1.14 parturitions, 3.12±1.42-year old), whereas LY cows were born from the oldest (2.72±1.80 parturitions, 3.97±2.01-year old; P<0.001). Life performance of the dams did not differ among clusters. In addition, metabolic parameters (fat and protein yield) were found to correlate significantly with yields between the first and second lactations (milk yield: r=0.357; fat yield: r=0.211; protein yield: r=0.277; P<0.0001), suggesting the influence of the individual. These results suggest that under optimal health, nutritional and environmental conditions, maternal aging is an important determinant of the life performance of progeny and argue for the need to identify conditions that contribute to health and disease in progeny according to the Developmental Origin of Health and Disease or DOHaD concept. Our findings may help the development of novel management guidelines for dairy farms.

  16. Recurrent aphthous stomatitis. An update.

    Science.gov (United States)

    Ship, J A

    1996-02-01

    Recurrent aphthous ulceration or recurrent aphthous stomatitis is the most common oral mucosal disease known to human beings. Despite much clinical and research attention, the causes remain poorly understood, the ulcers are not preventable, and treatment is symptomatic. The most common presentation is minor recurrent aphthous stomatitis: recurrent, round, clearly defined, small, painful ulcers that heal in 10 to 14 days without scarring. Major recurrent aphthous stomatitis lesions are larger (greater than 5 mm), can last for 6 weeks or longer, and frequently scar. The third variety of recurrent aphthous stomatitis is herpetiform ulcers, which present as multiple small clusters of pinpoint lesions that can coalesce to form large irregular ulcers and last 7 to 10 days. Diagnosis of all varieties is usually made after clinical examination. Many local and systemic factors have been associated with these conditions, and there is evidence that there may be a genetic and immunopathogenic basis for recurrent aphthous ulceration. Management of this condition depends on the clinical presentation and symptoms and includes analgesic, antimicrobial, and immunomodulatory drugs. As dental clinicians and researchers become better trained in oral medicine and stomatology, it is anticipated that the pathophysiology, prevention, and treatment of recurrent aphthous ulceration will improve in the future.

  17. Recurrent Aphthous Stomatitis: A Review.

    Science.gov (United States)

    Edgar, Natalie Rose; Saleh, Dahlia; Miller, Richard A

    2017-03-01

    Aphthous stomatitis is a painful and often recurrent inflammatory process of the oral mucosa that can appear secondary to various well-defined disease processes. Idiopathic recurrent aphthous stomatitis is referred to as recurrent aphthous stomatitis. The differential diagnosis for recurrent aphthous ulcerations is extensive and ranges from idiopathic benign causes to inherited fever syndromes, to connective tissue disease, or even inflammatory bowel diseases. A thorough history and review of systems can assist the clinician in determining whether it is related to a systemic inflammatory process or truly idiopathic. Management of aphthous stomatitis is challenging. For recurrent aphthous stomatitis or recalcitrant aphthous stomatitis from underlying disease, first-line treatment consists of topical medications with use of systemic medications as necessary. Herein, the authors discuss the differential diagnosis and treatment ladder of aphthous stomatitis as described in the literature.

  18. [Recurrent tuberculosis in patients with coinfection by HIV].

    Science.gov (United States)

    García Ordóñez, M A; Martínez González, J; Orihuela Cañadas, F; Jiménez Oñate, F; Colmenero Castillo, J D

    2003-06-01

    Analysis and identification of factors associated with the tuberculosis (TB) recurrence in HIV-infected patients. The HIV-infected patients diagnosed with TB between 1995-2000 and with correct completion of the treatment were selected. There were compared those patients who presented a tuberculous disease recurrence with those which did not presented it. Multivariate analysis was carried out by logistic regression in order to identify factors associated with a new episode of TB. In the total sample of 223 diagnosed HIV-infected patients with TB, 159 (71.3%) patients completed adequately the treatment and were considered cured. The rest were excluded because of: death before completing the treatment (5.8%), lost in the follow-up (11.7%), therapeutic abandonment (9.4%), and therapeutic failure (1.8%). In the 159 patient final sample, 14 (8.8%) patients presented later recurrence. The patients with recurrent TB presented a greater degree of immunosuppression, more previous complications indicative of AIDS, and greater frequency of extrapulmonary TB in the initial episode. After the multivariate analysis, a count of lymphocytes CD4 lower than 100/mm3 in the first episode of TB was associated with recurrent tuberculous disease (odds ratio [OR]: 4,6; 95% confidence index [CI]: 1,3-18,2). Mortality was high (35.7%) in the patients with recurrent episodes. The recurrence of TB in patients coinfected by HIV occurs in individuals with profound immunossuppression and is associated to high mortality.

  19. Imaging of prostate cancer local recurrences: why and how?

    Energy Technology Data Exchange (ETDEWEB)

    Rouviere, Olivier; Lyonnet, Denis [Universite de Lyon, Lyon (France); Universite Lyon 1, Faculte de Medecine Lyon Nord (France); Service d' Imagerie Urinaire et Vasculaire, Hospices Civils de Lyon, Hopital Edouard Herriot, Lyon (France); INSERM U 556, Lyon (France); Vitry, Thierry [Service d' Imagerie Urinaire et Vasculaire, Hospices Civils de Lyon, Hopital Edouard Herriot, Lyon (France)

    2010-05-15

    Because prostate cancer local recurrences can be efficiently treated by salvage therapies, it becomes critical to detect them early. The first alert is the rise of the prostate specific antigen (PSA) level after the post-treatment nadir, which can correspond to a distant recurrence, a local recurrence or both. This so-called biochemical failure (BF) is defined as PSA level >0.2 ng/ml after radical prostatectomy (RP) and PSA level > nadir+2 ng/ml after radiotherapy. There is no consensual definition of BF after cryotherapy, high-intensity focused ultrasound (HIFU) ablation or brachytherapy. Local recurrences after RP are treated by radiotherapy, those after radiotherapy by RP, cryotherapy, brachytherapy or HIFU ablation. Recurrences after cryotherapy or HIFU ablation can be treated by a second session or radiotherapy. Recurrences after brachytherapy are difficult to treat. In patients with BF, MRI can detect local recurrences, whatever the initial treatment was. Dynamic contrast-enhanced MRI seems particularly accurate. The role of spectroscopy remains controversial. Ultrasound-based techniques are less accurate, but this may change with the advent of ultrasonic contrast media. These recent advances in imaging may improve the outcome of salvage therapies (by improving patient selection and treatment targeting) and should open the way to focal salvage treatments in the near future. (orig.)

  20. Imaging of prostate cancer local recurrences: why and how?

    Science.gov (United States)

    Rouvière, Olivier; Vitry, Thierry; Lyonnet, Denis

    2010-05-01

    Because prostate cancer local recurrences can be efficiently treated by salvage therapies, it becomes critical to detect them early. The first alert is the rise of the prostate specific antigen (PSA) level after the post-treatment nadir, which can correspond to a distant recurrence, a local recurrence or both. This so-called biochemical failure (BF) is defined as PSA level > 0.2 ng/ml after radical prostatectomy (RP) and PSA level > nadir + 2 ng/ml after radiotherapy. There is no consensual definition of BF after cryotherapy, high-intensity focused ultrasound (HIFU) ablation or brachytherapy. Local recurrences after RP are treated by radiotherapy, those after radiotherapy by RP, cryotherapy, brachytherapy or HIFU ablation. Recurrences after cryotherapy or HIFU ablation can be treated by a second session or radiotherapy. Recurrences after brachytherapy are difficult to treat. In patients with BF, MRI can detect local recurrences, whatever the initial treatment was. Dynamic contrast-enhanced MRI seems particularly accurate. The role of spectroscopy remains controversial. Ultrasound-based techniques are less accurate, but this may change with the advent of ultrasonic contrast media. These recent advances in imaging may improve the outcome of salvage therapies (by improving patient selection and treatment targeting) and should open the way to focal salvage treatments in the near future.

  1. Effects of oral, subchronic cadmium administration on fertility, prenatal and postnatal progeny development in rats.

    Science.gov (United States)

    Barański, B; Stetkiewicz, I; Sitarek, K; Szymczak, W

    1983-12-01

    Cadmium chloride was administered by gavage to female rats 5 days a week for 5 weeks, then during mating and gestation periods at doses of 0.04, 0.4, and 4 mg Cd/kg/day. Treatment with cadmium neither affected the survival and fertility of females, nor produced overt fetotoxic effects. Fetal cadmium concentration was not related to the level of exposure. Litter size, body weight gain and viability of offspring during 2 months after parturition were similar in all groups. The exploratory locomotor activity of 2-month-old males and females born to rats given 0.4 and 4 mg Cd/kg/day was significantly reduced. The progeny of cadmium-treated females showed decreased performance in the rotarod test. In general, the degree of behavioral impairment was dose-related.

  2. Interleukin-33: a mediator of inflammation targeting hematopoietic stem and progenitor cells and their progenies

    Directory of Open Access Journals (Sweden)

    Hongnga eLe

    2013-05-01

    Full Text Available Inflammation is defined as a physiological response initiated by a variety of conditions that cause insult to the body, such as infection and tissue injury. Inflammation is triggered by specialized receptors in the innate immune system, which recognized by microbial components known as pathogen-associated molecular patterns (PAMPs or endogenous signals produced by damaged cells (damage-associated molecular patterns, DAMPs. IL-33 is a cytokine that is released predominantly at the epithelial barrier when it is exposed to pathogens, allergens, or injury-inducing stimuli. IL-33 target cells are various, ranging from hematopoietic stem and progenitor cells (HSPCs and essentially all types of their progeny to many nonhematopoietic cells. The pleiotrophic actions of IL-33 suggest that IL-33 is involved in every phase of the inflammatory process. In this review, we discuss recent advances in the understanding of how IL-33 orchestrates inflammatory responses by regulating HSPCs and innate immune cells.

  3. Evaluation of Antioxidant Compounds and Total Sugar Content in a Nectarine [Prunus persica (L. Batsch] Progeny

    Directory of Open Access Journals (Sweden)

    Yolanda Gogorcena

    2011-10-01

    Full Text Available Epidemiological studies suggest that consumption of fruit rich in phenolic compounds is associated with health-protective effects due to their antioxidant properties. For these reasons quality evaluation has become an important issue in fruit industry and in breeding programs. Phytochemical traits such as total phenolics, flavonoids, anthocyanins, L-ascorbic acid, sugar content and relative antioxidant capacity (RAC were analyzed over four years in flesh fruit of an F1 population “Venus” × “Big Top” nectarines. Other traits such as harvesting date, yield, fruit weight, firmness, soluble solids concentration (SSC, pH, titratable acidity (TA and ripening index (RI were also determined in the progeny. Results showed high variability among genotypes for all analyzed traits. Total phenolics and flavonoids showed significant positive correlations with RAC implying that both are important antioxidant bioactive compounds in peaches. We found genotypes with enhanced antioxidant capacity and a better performance than progenitors, and in consequence the best marketability.

  4. Evaluation of antioxidant compounds and total sugar content in a nectarine [Prunus persica (L.) Batsch] progeny.

    Science.gov (United States)

    Abidi, Walid; Jiménez, Sergio; Moreno, María Ángeles; Gogorcena, Yolanda

    2011-01-01

    Epidemiological studies suggest that consumption of fruit rich in phenolic compounds is associated with health-protective effects due to their antioxidant properties. For these reasons quality evaluation has become an important issue in fruit industry and in breeding programs. Phytochemical traits such as total phenolics, flavonoids, anthocyanins, L-ascorbic acid, sugar content and relative antioxidant capacity (RAC) were analyzed over four years in flesh fruit of an F1 population "Venus" × "Big Top" nectarines. Other traits such as harvesting date, yield, fruit weight, firmness, soluble solids concentration (SSC), pH, titratable acidity (TA) and ripening index (RI) were also determined in the progeny. Results showed high variability among genotypes for all analyzed traits. Total phenolics and flavonoids showed significant positive correlations with RAC implying that both are important antioxidant bioactive compounds in peaches. We found genotypes with enhanced antioxidant capacity and a better performance than progenitors, and in consequence the best marketability.

  5. Serological profiles of commercial broiler breeders and their progeny. 2. Newcastle disease virus.

    Science.gov (United States)

    King, D J

    1986-01-01

    Newcastle disease virus (NDV) hemagglutination-inhibition (HI) titers were determined for serum samples from eight commercial broiler breeder flocks and their progeny. The chickens sampled had been vaccinated and reared by different producers in different regions of the United States. Breeder flocks had the highest number of NDV-positive HI titers (greater than or equal to 1:10). Eighty percent or more of the samples from six of eight breeder flocks were positive; the geometric mean titers (GMTs) for those six breeder flocks ranged from 19 to 92. Only 3 of 8 broiler flocks had an increased frequency of positive titers and higher GMTs after vaccination. The frequency of positive titers was greater than 80% in only 2 of 8 of the oldest broiler flocks. The number of NDV-negative titers (less than 1:10) increased with age in most broiler flocks, even though all had been vaccinated once or more with live NDV vaccines.

  6. Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate

    Science.gov (United States)

    Laresgoiti, Usua; Rao, Chandrika; Brady, Jane L.; Richardson, Rachel V.; Batchen, Emma J.; Chapman, Karen E.

    2016-01-01

    Insufficient alveolar gas exchange capacity is a major contributor to lung disease. During lung development, a population of distal epithelial progenitors first produce bronchiolar-fated and subsequently alveolar-fated progeny. The mechanisms controlling this bronchiolar-to-alveolar developmental transition remain largely unknown. We developed a novel grafting assay to test if lung epithelial progenitors are intrinsically programmed or if alveolar cell identity is determined by environmental factors. These experiments revealed that embryonic lung epithelial identity is extrinsically determined. We show that both glucocorticoid and STAT3 signalling can control the timing of alveolar initiation, but that neither pathway is absolutely required for alveolar fate specification; rather, glucocorticoid receptor and STAT3 work in parallel to promote alveolar differentiation. Thus, developmental acquisition of lung alveolar fate is a robust process controlled by at least two independent extrinsic signalling inputs. Further elucidation of these pathways might provide therapeutic opportunities for restoring alveolar capacity. PMID:27578791

  7. A Truncated Nef Peptide from SIVcpz Inhibits the Production of HIV-1 Infectious Progeny

    Directory of Open Access Journals (Sweden)

    Marcela Sabino Cunha

    2016-07-01

    Full Text Available Nef proteins from all primate Lentiviruses, including the simian immunodeficiency virus of chimpanzees (SIVcpz, increase viral progeny infectivity. However, the function of Nef involved with the increase in viral infectivity is still not completely understood. Nonetheless, until now, studies investigating the functions of Nef from SIVcpz have been conducted in the context of the HIV-1 proviruses. In an attempt to investigate the role played by Nef during the replication cycle of an SIVcpz, a Nef-defective derivative was obtained from the SIVcpzWTGab2 clone by introducing a frame shift mutation at a unique restriction site within the nef sequence. This nef-deleted clone expresses an N-terminal 74-amino acid truncated peptide of Nef and was named SIVcpz-tNef. We found that the SIVcpz-tNef does not behave as a classic nef-deleted HIV-1 or simian immunodeficiency virus of macaques SIVmac. Markedly, SIVcpz-tNef progeny from both Hek-293T and Molt producer cells were completely non-infectious. Moreover, the loss in infectivity of SIVcpz-tNef correlated with the inhibition of Gag and GagPol processing. A marked accumulation of Gag and very low levels of reverse transcriptase were detected in viral lysates. Furthermore, these observations were reproduced once the tNef peptide was expressed in trans both in SIVcpzΔNef and HIV-1WT expressing cells, demonstrating that the truncated peptide is a dominant negative for viral processing and infectivity for both SIVcpz and HIV-1. We demonstrated that the truncated Nef peptide binds to GagPol outside the protease region and by doing so probably blocks processing of both GagPol and Gag precursors at a very early stage. This study demonstrates for the first time that naturally-occurring Nef peptides can potently block lentiviral processing and infectivity.

  8. Variations in oil palm (Elaeis guineensis Jacq.) progeny response to high aluminium concentrations in solution culture.

    Science.gov (United States)

    Cristancho, R J A; Hanafi, M M; Omar, S R Syed; Rafii, M Y

    2011-03-01

    Aluminium (Al) phytotoxicity is an important soil constraint that limits crop yield. The objectives of this study were to investigate how growth, physiology, nutrient content and organic acid concentration is affected by Al, and to assess the degree of Al tolerance in different oil palm progeny (OPP). Four OPPs ['A' (Angola dura × Angola dura), 'B' (Nigerian dura × Nigerian dura), 'C' (Deli dura × AVROS pisifera) and 'D' (Deli dura × Dumpy AVROS pisifera)] were grown in different Al concentrations (0, 100 and 200 μm) in aerated Hoagland solution, pH 4.4, for 80 days. We observed a severe reduction (57.5%) in shoot dry weight, and root tips were reduced by 46.5% in 200 μm Al. In 'B' and 'C', the majority of macro- and micronutrients in plants were reduced significantly by 200 μm Al, with Mg being lowered by more than 50% in roots and shoots. The 200 μm Al treatment resulted in a 56.50% reduction in total leaf area, a 20% reduction in net photosynthesis and a 17% reduction in SPAD chlorophyll value in the third leaf. Root tips (0-5 mm) showed a significant increase in oxalic acid content with increasing Al concentration (∼ 5.86-fold); progeny 'A' had the highest concentration of oxalic acid. There was a significant interaction between Al concentration × OPP on total leaf number, root volume, lateral root length, Mg and K in root and shoot tissues, and Ca and N in shoots. The OPPs could be ranked in their tolerance to Al as: 'A' > 'D' > 'B' > 'C'. © 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.

  9. Responses of human embryonic stem cells and their differentiated progeny to ionizing radiation

    Energy Technology Data Exchange (ETDEWEB)

    Zou, Ying; Zhang, Ningzhe; Ellerby, Lisa M.; Davalos, Albert R.; Zeng, Xianmin; Campisi, Judith [Buck Institute for Research on Aging, Novato, CA 94945 (United States); Desprez, Pierre-Yves, E-mail: pydesprez@cpmcri.org [Buck Institute for Research on Aging, Novato, CA 94945 (United States); California Pacific Medical Center, Research Institute, San Francisco, CA 94107 (United States)

    2012-09-14

    Highlights: Black-Right-Pointing-Pointer hESCs and their progeny, NSCs and neurons, were exposed to ionizing radiation. Black-Right-Pointing-Pointer Upon irradiation, most hESCs died within 5-7 h. Black-Right-Pointing-Pointer Surviving NSCs underwent senescence and displayed features of astrocytes. Black-Right-Pointing-Pointer Surviving NSCs did not display the secretory phenotype expressed by pure astrocytes. Black-Right-Pointing-Pointer This study is to better understand the stress-responses of hESCs and their progeny. -- Abstract: Human embryonic stem cells (hESCs) hold promise for the treatment of many human pathologies. For example, hESCs and the neuronal stem cells (NSCs) and neurons derived from them have significant potential as transplantation therapies for a variety of neurodegenerative diseases. Two concerns about the use of hESCs and their differentiated derivatives are their ability to function and their ability to resist neoplastic transformation in response to stresses that inevitably arise during their preparation for transplantation. To begin to understand how these cells handle genotoxic stress, we examined the responses of hESCs and derived NSCs and neurons to ionizing radiation (IR). Undifferentiated hESCs were extremely sensitive to IR, with nearly all the cells undergoing cell death within 5-7 h. NSCs and neurons were substantially more resistant to IR, with neurons showing the most resistant. Of interest, NSCs that survived IR underwent cellular senescence and acquired astrocytic characteristics. Unlike IR-treated astrocytes, however, the NSC-derived astrocytic cells that survived IR did not display the typical pro-inflammatory, pro-carcinogenic senescence-associated secretory phenotype. These findings suggest distinct genotoxic stress-responses of hESCs and derived NSC and neuronal populations, and suggest that damaged NSCs, while failing to function, may not cause local inflammation.

  10. Identification of drought tolerant progenies in tea by gene expression analysis.

    Science.gov (United States)

    Gupta, Sushmita; Bharalee, Raju; Bhorali, Priyadarshini; Bandyopadhyay, Tirthankar; Gohain, Bornali; Agarwal, Niraj; Ahmed, Parveen; Saikia, Hemanta; Borchetia, Sangeeta; Kalita, M C; Handique, A K; Das, Sudripta

    2012-08-01

    Understanding the genes that govern tea plant (Camellia sinensis) architecture and response to drought stress is urgently needed to enhance breeding in tea with improved water use efficiency. Field drought is a slow mechanism and the plants go through an adaptive process in contrast to the drastic changes of rapid dehydration in case of controlled experiments. We identified a set of drought responsive genes under controlled condition using SSH, and validated the identified genes and their pattern of expression under field drought condition. The study was at three stages of water deficit stress viz., before wilting, wilting and recovery, which revealed a set of genes with higher expression at before wilting stage including dehydrin, abscissic acid ripening protein, glutathione peroxidase, cinnamoyl CoA reductase, calmodulin binding protein. The higher expression of these genes was related with increase tolerance character of DT/TS-463 before wilting, these five tolerant progenies could withstand drought stress and thus are candidates for breeding. We observed that physiological parameter like water use efficiency formed a close group with genes such as calmodulin related, DRM3, hexose transporter, hydrogen peroxide induced protein, ACC oxidase, lipase, ethylene responsive transcription factor and diaminopimelate decarboxylase, during wilting point. Our data provides valuable information for the gene components and the dynamics of gene expression in second and third leaf against drought stress in tea, which could be regarded as candidate targets potentially associated with drought tolerance. We propose that the identified five tolerant progenies on the basis of their drought tolerance can thus be utilised for future breeding programmes.

  11. A European-wide 222radon and 222radon progeny comparison study

    Science.gov (United States)

    Schmithüsen, Dominik; Chambers, Scott; Fischer, Bernd; Gilge, Stefan; Hatakka, Juha; Kazan, Victor; Neubert, Rolf; Paatero, Jussi; Ramonet, Michel; Schlosser, Clemens; Schmid, Sabine; Vermeulen, Alex; Levin, Ingeborg

    2017-04-01

    Although atmospheric 222radon (222Rn) activity concentration measurements are currently performed worldwide, they are being made by many different laboratories and with fundamentally different measurement principles, so compatibility issues can limit their utility for regional-to-global applications. Consequently, we conducted a European-wide 222Rn / 222Rn progeny comparison study in order to evaluate the different measurement systems in use, determine potential systematic biases between them, and estimate correction factors that could be applied to harmonize data for their use as a tracer in atmospheric applications. Two compact portable Heidelberg radon monitors (HRM) were moved around to run for at least 1 month at each of the nine European measurement stations included in this comparison. Linear regressions between parallel data sets were calculated, yielding correction factors relative to the HRM ranging from 0.68 to 1.45. A calibration bias between ANSTO (Australian Nuclear Science and Technology Organisation) two-filter radon monitors and the HRM of ANSTO / HRM = 1.11 ± 0.05 was found. Moreover, for the continental stations using one-filter systems that derive atmospheric 222Rn activity concentrations from measured atmospheric progeny activity concentrations, preliminary 214Po / 222Rn disequilibrium values were also estimated. Mean station-specific disequilibrium values between 0.8 at mountain sites (e.g. Schauinsland) and 0.9 at non-mountain sites for sampling heights around 20 to 30 m above ground level were determined. The respective corrections for calibration biases and disequilibrium derived in this study need to be applied to obtain a compatible European atmospheric 222Rn data set for use in quantitative applications, such as regional model intercomparison and validation or trace gas flux estimates with the radon tracer method.

  12. Epigenetic, histopathological and transcriptomic effects following exposure to depleted uranium in adult zebrafish and their progeny

    Energy Technology Data Exchange (ETDEWEB)

    Gombeau, Kewin, E-mail: kewin.gombeau@gmail.com [Institut de Radioprotection et de SÛreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, Saint-Paul-lez-Durance 13115 (France); Bourdineaud, Jean-Paul, E-mail: jean-paul.bourdineaud@u-bordeaux.fr [Université de Bordeaux, CNRS, UMR 5805, EPOC, 33400 Talence (France); Ravanat, Jean-Luc, E-mail: jean-luc.ravanat@cea.fr [Univ. Grenoble Alpes, INAC-SyMMES, 38000 Grenoble (France); CEA, INAC-SCIB Laboratoire des Lésions des Acides Nucléiques, 38000 Grenoble (France); Armant, Olivier, E-mail: olivier.armant@irsn.fr [Institut de Radioprotection et de SÛreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, Saint-Paul-lez-Durance 13115 (France); Camilleri, Virginie, E-mail: virginie.camilleri@irsn.fr [Institut de Radioprotection et de SÛreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, Saint-Paul-lez-Durance 13115 (France); Cavalie, Isabelle, E-mail: isabelle.cavalie@irsn.fr [Institut de Radioprotection et de SÛreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, Saint-Paul-lez-Durance 13115 (France); Floriani, Magali, E-mail: magali.floriani@irsn.fr [Institut de Radioprotection et de SÛreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, Saint-Paul-lez-Durance 13115 (France); and others

    2017-03-15

    Highlights: • The parental DU-exposure induced a significant transfer of uranium into eggs. • Du-exposed progeny exhibited a significant 2-fold increased DNA methylation level. • The transcriptomic response was deeply modified in the DU-exposed organisms. • DU-exposed adult and offspring presented significant histopathological injuries. - Abstract: This study investigated the effects of adult zebrafish exposure to a nominal concentration of 20 μg L{sup −1} of depleted uranium (DU) for six days upon DNA methylation, gene expression and the appearance of histopathological damage in their progeny. In the embryos at the 2–8 cell stage, the parental exposure induced significant DU accumulation, with levels seven times higher than those measured in the control embryos, but in larvae 96 h post-fertilisation (hpf), uranium concentration had already returned to a level identical to that of the control larvae. A significant two-fold increase in the global level of DNA methylation was observed in embryos as early as the prim5 (24 hpf) stage and was still maintained at the 96 hpf stage despite the fact that DU had already been depurated at the later stage. RNA sequencing analysis indicated an impact of parental exposure upon the total RNAs transmitted from the mother to eggs, and the up-regulated genes were those associated with post-traductional protein modification and trafficking and cellular signalling pathways, whereas the down-regulated genes concerned the translational process, cell cycle regulation and several cell signalling pathways. Alterations of photoreceptor cells and the axon-axon junctions between photoreceptors were observed in the eyes of adult fish exposed for 10 days to DU. Actin and myosin filament disorganisation was observed in the skeletal muscles of 96 hpf larvae, at a stage when the maternally transmitted DU had already been excreted. These data reveal the extreme sensitivity of zebrafish embryos to DU transmitted through the oocyte by

  13. iTRAQ-based proteomic analysis of polyploid giant cancer cells and budding progeny cells reveals several distinct pathways for ovarian cancer development.

    Directory of Open Access Journals (Sweden)

    Shiwu Zhang

    Full Text Available Polyploid giant cancer cells (PGCCs are a morphologically distinct subgroup of human tumor cells with increased nuclear size or multiple nuclei, but they are generally considered unimportant because they are presumed to be nondividing and thus nonviable. We have recently shown that these large cancer cells are not only viable but also can divide asymmetrically and yield progeny cancer cells with cancer stem-like properties via budding division. To further understand the molecular events involved in the regulation of PGCCs and the generation of their progeny cancer cells, we comparatively analyzed the proteomic profiles of PGCCs, PGCCs with budding daughter cells, and regular control cancer cells from the HEY and SKOv3 human ovarian cancer cell lines with and without CoCl2. We used a high-throughput iTRAQ-based proteomic methodology coupled with liquid chromatography-electrospray ionization tandem mass spectroscopy to determine the differentiated regulated proteins. We performed Western blotting and immunohistochemical analyses to validate the differences in the expression patterns of a variety of proteins between PGCCs or budding PGCCs and regular cancer cells identified by iTRAQ approach and also a selected group of proteins from the literature. The differentially regulated proteins included proteins involved in response to hypoxia, stem cell generation, chromatin remodeling, cell-cycle regulation, and invasion and metastasis. In particular, we found that HIF-1alpha and its known target STC1 are upregulated in PGCCs. In addition, we found that a panel of stem cell-regulating factors and epithelial-to-mesenchymal transition regulatory transcription factors were upregulated in budding PGCCs, whereas expression of the histone 1 family of nucleosomal linker proteins was consistently lower in PGCCs than in control cells. Thus, proteomic expression patterns provide valuable insight into the underlying mechanisms of PGCC formation and the relationship

  14. White oak growth after 23 Years in a three-site provenance/progeny trial on a latitudinal gradient in Indiana

    Science.gov (United States)

    Yen-Ning Huang; Hao Zhang; Scott Rogers; Mark Coggeshall; Keith Woeste

    2015-01-01

    To increase the availability of improved, adapted white oak (Quercus alba L.) for midwestern United States landowners, we analyzed data from three 23-year-old provenance/progeny tests of 70 open-pollinated progenies from 17 provenances. Our goal was to estimate the heritability of height growth and range of adaptation and ultimately to determine...

  15. Recurrent mild cerebral ischemia: enhanced brain injury following acute compared to subacute recurrence in the rat

    OpenAIRE

    Ursula I. Tuor; Zhao, Zonghang; Barber, Philip A.; Qiao, Min

    2016-01-01

    Background In the current study, a transient cerebral ischemia producing selective cell death was designated a mild ischemic insult. A comparable insult in humans is a transient ischemic attack (TIA) that is associated with functional recovery but can have imaging evidence of minor ischemic damage including cerebral atrophy. A TIA also predicts a high risk for early recurrence of a stroke or TIA and thus multiple ischemic insults are not uncommon. Not well understood is what the effect of dif...

  16. Recurrent aphthous stomatitis.

    Science.gov (United States)

    Cui, Ricky Z; Bruce, Alison J; Rogers, Roy S

    2016-01-01

    Recurrent aphthous stomatitis (RAS) is the most common acute oral ulcerative condition in North America. RAS is divided into a mild, common form, simple aphthosis, and a severe, less common form, complex aphthosis. Aphthosis is a reactive condition. The lesions of RAS can represent the mucosal manifestation of a variety of conditions. These include conditions with oral and genital aphthae such as ulcus vulvae acutum, reactive nonsexually related acute genital ulcers, and Behçet disease. The mouth is the beginning of the gastrointestinal (GI) tract, and the lesions of RAS can be a manifestation of GI diseases such as gluten-sensitive enteropathy, ulcerative colitis, and Crohn disease. Complex aphthosis may also have correctable causes. The clinician should seek these in a careful evaluation. Successful management of both simple and complex aphthosis depends on accurate diagnosis, proper classification, recognition of provocative factors, and the identification of associated diseases. The outlook for patients with both simple and complex aphthosis is positive. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. No significant level of inheritable interchromosomal aberrations in the progeny of bystander primary human fibroblasts after alpha particle irradiation

    Science.gov (United States)

    Hu, Burong; Zhu, Jiayun; Zhou, Hongning; Hei, Tom K.

    2013-02-01

    A major concern for bystander effects is the probability that normal healthy cells adjacent to the irradiated cells become genomically unstable and undergo further carcinogenesis after therapeutic irradiation or space mission where astronauts are exposed to low dose of heavy ions. Genomic instability is a hallmark of cancer cells. In the present study, two irradiation protocols were performed in order to ensure pure populations of bystander cells and the genomic instability in their progeny were investigated. After irradiation, chromosomal aberrations of cells were analyzed at designated time points using G2 phase premature chromosome condensation (G2-PCC) coupled with Giemsa staining and with multiplex fluorescent in situ hybridization (mFISH). Our Giemsa staining assay demonstrated that elevated yields of chromatid breaks were induced in the progeny of pure bystander primary fibroblasts up to 20 days after irradiation. mFISH assay showed no significant level of inheritable interchromosomal aberrations were induced in the progeny of the bystander cell groups, while the fractions of gross aberrations (chromatid breaks or chromosomal breaks) significantly increased in some bystander cell groups. These results suggest that genomic instability occurred in the progeny of the irradiation associated bystander normal fibroblasts exclude the inheritable interchromosomal aberration.

  18. Family-site interaction in Pinus radiata: implications for progeny testing strategy and regionalised breeding in New Zealand.

    Science.gov (United States)

    G.R. Johnson; R.D. Brudon

    1990-01-01

    A progeny test of 170 open-pollinated families from second-generation plus trees of Pinus radiata was established on four sites in New Zealand in 1981. Two test sites were on volcanic purnice soils in the Central North Island region and two were on phosphate-retentive clay soils in the Northland region.Assessments of volume growth, stem straightness, mal-...

  19. Evaluation of human embryonic stem cells and their differentiated fibroblastic progenies as cellular models for in vitro genotoxicity screening.

    Science.gov (United States)

    Vinoth, Kumar Jayaseelan; Manikandan, Jayapal; Sethu, Swaminathan; Balakrishnan, Lakshmidevi; Heng, Alexis; Lu, Kai; Hande, Manoor Prakash; Cao, Tong

    2014-08-20

    This study evaluated human embryonic stem cells (hESC) and their differentiated fibroblastic progenies as cellular models for genotoxicity screening. The DNA damage response of hESCs and their differentiated fibroblastic progenies were compared to a fibroblastic cell line (HEPM, CRL1486) and primary cultures of peripheral blood lymphocytes (PBL), upon exposure to Mitomycin C, gamma irradiation and H2O2. It was demonstrated that hESC-derived fibroblastic progenies (H1F) displayed significantly higher chromosomal aberrations, micronuclei formation and double strand break (DSB) formation, as compared to undifferentiated hESC upon exposure to genotoxic stress. Nevertheless, H1F cell types displayed comparable sensitivities to genotoxic challenge as HEPM and PBL, both of which are representative of somatic cell types commonly used for genotoxicity screening. Subsequently, transcriptomic and pathways analysis identified differential expression of critical genes involved in cell death and DNA damage response upon exposure to gamma irradiation. The results thus demonstrate that hESC-derived fibroblastic progenies are as sensitive as commonly-used somatic cell types for genotoxicity screening. Moreover, hESCs have additional advantages, such as their genetic normality compared to immortalized cell lines, as well as their amenability to scale-up for producing large, standardized quantities of cells for genotoxicity screening on an industrial scale, something which can never be achieved with primary cell cultures. Copyright © 2014. Published by Elsevier B.V.

  20. Simultaneous Visualization of Parental and Progeny Viruses by a Capsid-Specific HaloTag Labeling Strategy.

    Science.gov (United States)

    Liu, An-An; Zhang, Zhenfeng; Sun, En-Ze; Zheng, Zhenhua; Zhang, Zhi-Ling; Hu, Qinxue; Wang, Hanzhong; Pang, Dai-Wen

    2016-01-26

    Real-time, long-term, single-particle tracking (SPT) provides us an opportunity to explore the fate of individual viruses toward understanding the mechanisms underlying virus infection, which in turn could lead to the development of therapeutics against viral diseases. However, the research focusing on the virus assembly and egress by SPT remains a challenge because established labeling strategies could neither specifically label progeny viruses nor make them distinguishable from the parental viruses. Herein, we have established a temporally controllable capsid-specific HaloTag labeling strategy based on reverse genetic technology. VP26, the smallest pseudorabies virus (PrV) capsid protein, was fused with HaloTag protein and labeled with the HaloTag ligand during virus replication. The labeled replication-competent recombinant PrV harvested from medium can be applied directly in SPT experiments without further modification. Thus, virus infectivity, which is critical for the visualization and analysis of viral motion, is retained to the largest extent. Moreover, progeny viruses can be distinguished from parental viruses using diverse HaloTag ligands. Consequently, the entire course of virus infection and replication can be visualized continuously, including virus attachment and capsid entry, transportation of capsids to the nucleus along microtubules, docking of capsids on the nucleus, endonuclear assembly of progeny capsids, and the egress of progeny viruses. In combination with SPT, the established strategy represents a versatile means to reveal the mechanisms and dynamic global picture of the life cycle of a virus.

  1. Combining ability of S3 progenies for key agronomic traits in popcorn: comparison of testers in top-crosses.

    Science.gov (United States)

    de Lima, V J; do Amaral Junior, A T; Kamphorst, S H; Pena, G F; Leite, J T; Schmitt, K F M; Vittorazzi, C; de Almeida Filho, J E; Mora, F

    2016-12-02

    The successful development of hybrid cultivars depends on the reliability of estimated combining ability of the parent lines. The objectives of this study were to assess the combining ability of partially inbred S3 families of popcorn derived from the open-pollinated variety UENF 14, via top-crosses with four testers, and to compare the testers for their ability to discriminate the S3 progenies. The experiment was conducted in the 2015/2016 crop season, in an incomplete-block (Lattice) design with three replications. The following agronomic traits were evaluated: average plant height, grain yield (GY), popping expansion (PE), and expanded popcorn volume per hectare. The top-cross hybrid, originating from the BRS-Angela vs S3 progeny 10 combination, was indicated as promising, showing high values for specific combining ability for GY and PE. For the S3 progenies that showed high and positive GCA values for GY and PE, the continuity of the breeding program is recommended, with the advance of self-pollination generations. Fasoulas' differentiation index discriminated the BRS-Angela tester as the most suitable for identifying the superior progenies.

  2. Effect of maternal restraint stress during gestation on temporal lipopolysaccharide-induced neuroendocrine and immune responses of progeny

    Science.gov (United States)

    The impact of gestational dam restraint stress on progeny immune and neuroendocrine temporal hormone responses to lipopoly-saccharide (LPS) challenge was assessed. Maternal stress (5-min snout snare restraint stress during days 84 to 112 of gestation) increased (P < 0.05) the magnitude of tumor necr...

  3. Assessment of glucose homeostasis in crossbred steer progeny sired by Brahman bulls that experienced prenatal transportation stress

    Science.gov (United States)

    The objective of this experiment was to assess glucose homeostasis of crossbred male progeny whose Brahman sires experienced prenatal transportation stress (PS) in utero. Sixteen steers (PNS group) sired by 3 PS bulls gestating dams were transported for 2 h at 60, 80, 100, 120, and 140 ± 5 d of gest...

  4. Variação genética em procedências e progênies mexicanas de Pinus maximinoi. Genetic variation in Mexican provenances and progenies of Pinus maximinoi.

    Directory of Open Access Journals (Sweden)

    Lígia de Castro ETTORI

    2004-06-01

    selection in this level. P. maximinoi from any of the provenances represented in this study can be recommended for commercial plantation in Angatuba region. Its performance was comparable to that of P. oocarpa used as control species. The estimates of genetic variation indicated that most of them are distributed among trees within progenies and the highest heritability was observed at progeny level. The results suggest that the greatest genetic gains can be reached through progenies selection followed by selection within progenies. The greatest response to selection was estimated in DBH with potential gain in the order of 8.4% at eleven years old.

  5. Management Options for Biochemically Recurrent Prostate Cancer.

    Science.gov (United States)

    Fakhrejahani, Farhad; Madan, Ravi A; Dahut, William L

    2017-05-01

    Prostate cancer is the most common solid tumor malignancy in men worldwide. Treatment with surgery and radiation can be curative in organ-confined disease. Unfortunately, about one third of men develop biochemically recurrent disease based only on rising prostate-specific antigen (PSA) in the absence of visible disease on conventional imaging. For these patients with biochemical recurrent prostate cancer, there is no uniform guideline for subsequent management. Based on available data, it seems prudent that biochemical recurrent prostate cancer should initially be evaluated for salvage radiation or prostatectomy, with curative intent. In selected cases, high-intensity focused ultrasound and cryotherapy may be considered in patients that meet very narrow criteria as defined by non-randomized trials. If salvage options are not practical or unsuccessful, androgen deprivation therapy (ADT) is a standard option for disease control. While some patients prefer ADT to manage the disease immediately, others defer treatment because of the associated toxicity. In the absence of definitive randomized data, patients may be followed using PSA doubling time as a trigger to initiate ADT. Based on retrospective data, a PSA doubling time of less than 3-6 months has been associated with near-term development of metastasis and thus could be used signal to initiate ADT. Once treatment is begun, patients and their providers can choose between an intermittent and continuous ADT strategy. The intermittent approach may limit side effects but in patients with metastatic disease studies could not exclude a 20% greater risk of death. In men with biochemical recurrence, large studies have shown that intermittent therapy is non-inferior to continuous therapy, thus making this a reasonable option. Since biochemically recurrent prostate cancer is defined by technological limitations of radiographic detection, as new imaging (i.e., PSMA) strategies are developed, it may alter how the disease is

  6. STDP in recurrent neuronal networks

    Directory of Open Access Journals (Sweden)

    Matthieu Gilson

    2010-09-01

    Full Text Available Recent results about spike-timing-dependent plasticity (STDP in recurrently connected neurons are reviewed, with a focus on the relationship between the weight dynamics and the emergence of network structure. In particular, the evolution of synaptic weights in the two cases of incoming connections for a single neuron and recurrent connections are compared and contrasted. A theoretical framework is used that is based upon Poisson neurons with a temporally inhomogeneous firing rate and the asymptotic distribution of weights generated by the learning dynamics. Different network configurations examined in recent studies are discussed and an overview of the current understanding of STDP in recurrently connected neuronal networks is presented.

  7. [Recurrent aphthous stomatitis in Rheumatology].

    Science.gov (United States)

    Riera Matute, Gabriel; Riera Alonso, Elena

    2011-01-01

    Recurrent aphthous stomatitis consists on recurring oral ulcers of unknown etiology. Oral ulcers may be different in number and size depending on the clinical presentation, which also determines the time needed for healing. Moreover, there are factors associated to outbreaks but not implicated in its etiopathogenesis. When oral aphthosis has a known etiology, it is not considered as recurrent aphthous stomatitis. The severity and the clinical presentation helps in the differential diagnosis. Treatment is symptomatic in recurrent aphthous stomatitis while, if there is an underlying systemic disease, the treatment of such disease is need in addition to topical treatment. Copyright © 2011 Elsevier España, S.L. All rights reserved.

  8. Recurrent networks for wave forecasting

    Digital Repository Service at National Institute of Oceanography (India)

    Mandal, S.; Prabaharan, N.

    , merchant vessel routing, nearshore construction, etc. more efficiently and safely. This paper presents an application of the Artificial Neural Network, namely Backpropagation Recurrent Neural Network (BRNN) with rprop update algorithm for wave forecasting...

  9. Opioids and breast cancer recurrence

    DEFF Research Database (Denmark)

    Cronin-Fenton, Deirdre P; Heide-Jørgensen, Uffe; Ahern, Thomas P

    2015-01-01

    BACKGROUND: Opioids may alter immune function, thereby potentially affecting cancer recurrence. The authors investigated the association between postdiagnosis opioid use and breast cancer recurrence. METHODS: Patients with incident, early stage breast cancer who were diagnosed during 1996 through...... 2008 in Denmark were identified from the Danish Breast Cancer Cooperative Group Registry. Opioid prescriptions were ascertained from the Danish National Prescription Registry. Follow-up began on the date of primary surgery for breast cancer and continued until breast cancer recurrence, death......, emigration, 10 years, or July 31, 2013, whichever occurred first. Cox regression models were used to compute hazard ratios and 95% confidence intervals associating breast cancer recurrence with opioid prescription use overall and by opioid type and strength, immunosuppressive effect, chronic use (≥6 months...

  10. Recurrent Shoulder Instability in a Young, Active, Military Population and Its Professional Implications.

    Science.gov (United States)

    Flint, James H; Pickett, Adam; Owens, Brett D; Svoboda, Steven J; Peck, Karen Y; Cameron, Kenneth L; Biery, John; Giuliani, Jeffrey; Rue, John-Paul

    Shoulder instability is a topic of significant interest within the sports medicine literature, particularly regarding recurrence rates and the ideal treatment indications and techniques. Little has been published specifically addressing the occupational implications of symptomatic recurrent shoulder instability. Previous arthroscopic repair will continue to be a significant predisposing factor for recurrent instability in a young, active population, and that recurrent instability may have a negative effect on college graduation and postgraduate occupational selection. Case series. Level 4. We conducted a retrospective review of approved medical waivers for surgical treatment of anterior shoulder dislocation or instability prior to matriculation at the US Military Academy or the US Naval Academy for the graduating classes of 2010 to 2013. Statistical analysis was performed to determine the incidence and risk factors for recurrence and to determine the impact on graduation rate and occupation selection. Fifty-nine patients were evaluated; 34% developed recurrent anterior instability. Patients with previous arthroscopic repair had a significantly higher incidence of recurrence (38%, P = 0.044). Recurrent shoulder instability did not significantly affect graduation rates or self-selected occupation ( P ≥ 0.05). There is a significant rate of recurrent shoulder instability after primary surgical repair, particularly among young, active individuals. In addition, arthroscopic repair resulted in a significantly higher recurrence rate compared with open repair in our population. Surgical repair for shoulder instability should not necessarily preclude young individuals from pursuing (or being considered for) occupations that may place them at greater risk of recurrence. The risk of recurrent instability is greater than the rate typically described, which may suggest that some subpopulations are at greater risk than others. A unique data point regarding instability is the

  11. Relationship of seed traits on initial progeny growth performance and divergence studies in Madhuca latifolia Macb. for further use in tree improvement

    Directory of Open Access Journals (Sweden)

    BN Divakara

    2014-12-01

    Full Text Available Evaluation of twenty-three genotypes of M. latifolia was carried out based on relationship of seed traits with initial progeny growth performance and divergence studies as a scope for further breeding programme. Variability studies revealed that, more than twelve accessions recorded above average for 100-seed weight (247.5±49.2, oil content (43.8±3.7 and volume index (346.0±97.7. The maximum values observed in studied CPTs were as follows: seed length (39.1 mm in CPT-15 genotype, seed breadth (19.2 mm in CPT–8 and CPT–9, aspect ratio (2.2 in CPT-6 and CPT-15, 2D surface area (501.4 and 491.6 mm2 in CPT-9 and CPT-3 respectively. CPT–16 recorded maximum for 100 seed weight (282.4 g and oil content (51.2%. However, maximum volume index was recorded by CPT–3 (578.3 cm3 followed by CPT–16 (496.0 cm3. The phenotypic and genotypic coefficients of variations are close to each other for all traits, except volume index that exhibited striking difference between PCV (40.0% and GCV (19.9% indicating that for most traits genetic control was quite high. Trait oil content and 100 seed weight expressed high heritability (93.5%, 93.0% accompanied with moderate genetic advance (17.2%, 15.6%, indicating that, heritability is due to additive gene effects and selection may be effective. At genotypic level 100 seed weight registered positive significant correlation with height (0.73 and seed breadth, oil content with volume index (0.55, 0.71. Hence seeds with large breadth, high seed weight and oil content may be selected for producing better progenies. Since traits viz. 100 seed weight and oil content are under strong genetic control, improvement in these characters can bring improvement in volume index. On the basis of the divergence, the 23 genotypes studied were grouped into 5 clusters, indicating wide diversity. The clustering pattern shows that geographical diversity is not necessarily related to genetic diversity. The genotypes in cluster IV and V

  12. Winter grazing system and supplementation of beef cows during late gestation influence heifer progeny.

    Science.gov (United States)

    Funston, R N; Martin, J L; Adams, D C; Larson, D M

    2010-12-01

    A 2 × 2 factorial arrangement of treatments was used to evaluate effects of dam winter grazing system and supplementation in the last third of gestation on subsequent BW gain, feed efficiency, and reproduction in heifer progeny. Crossbred cows (yr 1, n = 109; yr 2, n = 114; yr 3, n = 116) grazed range (WR) or corn residue (CR) during winter and within grazing treatment received 0.40 kg/d of 31% CP (DM basis) cubes (PS) or no supplement (NS). Heifer calves (yr 1, n = 56; yr 2, n = 56; yr 3, n = 54) grazed dormant pasture for 114 d postweaning and were individually fed for 87 d before a 45-d natural service breeding. Dam PS reduced (P = 0.04) heifer birth date and CR tended to increase (P = 0.07) heifer birth BW. Both PS and CR increased (P ≤ 0.05) heifer weaning BW; however, adjusted 205-d weaning BW was only lighter (P = 0.03) if the dam grazed WR with NS. Heifers from PS dams tended to be younger (P = 0.09) at puberty than NS, and there was a trend (P = 0.11) for more heifers to be pubertal by breeding if the dam grazed WR with PS compared with other treatment groups. Heifers from WR-NS dams tended to weigh less (P ≤ 0.09) at breeding and at pregnancy diagnosis than WR PS. There was a trend (P = 0.13) for pregnancy rate to be greater for heifers born to PS dams. Individually fed heifer DMI was not affected (P = 0.25) by treatment; however, heifers from dams that grazed CR with PS gained the least BW (P = 0.04) during individual feeding and had the smallest (P = 0.03) G:F. In contrast, there were no differences (P > 0.15) in feed efficiency when expressed as residual feed intake. The first calf birth and weaning BW of the heifer was unaffected (P > 0.15) by dam treatment. Heifers from dams that grazed WR with NS tended to have lighter (P = 0.09) BW before the second breeding season but similar (P = 0.97) pregnancy rates. Cows grazing CR with NS produced the most valuable heifer calf at weaning; however, heifers from cows that grazed WR with NS cost the least

  13. Recurrence of superficial vein thrombosis in patients with varicose veins.

    Science.gov (United States)

    Karathanos, Christos; Spanos, Konstantinos; Saleptsis, Vassileios; Tsezou, Aspasia; Kyriakou, Despina; Giannoukas, Athanasios D

    2016-08-01

    To investigate which factors other than history of superficial vein thrombosis (SVT) are associated with recurrent spontaneous SVT episodes in patients with varicose veins (VVs). Patients with a history of spontaneous SVT and VVs were followed up for a mean period of 55 months. Demographics, comorbidities, and thrombophilia screening test were analyzed. Patients were grouped according to the clinical-etiology-anatomy-pathophysiology classification. A multiple logistic regression analysis with the forward likelihood ratio method was undertaken. Thirteen patients out of 97 had a recurrence SVT episode during the follow-up period. All those patients were identified to have a thrombophilia defect. Protein C and S, antithrombin, and plasminogen deficiencies were more frequently present in patients without recurrence. Gene mutations were present in 38% in the nonrecurrence group and 77% in the recurrence group. After logistic regression analysis, patients with dislipidemia and mutation in prothrombin G20210A (FII) had an increased risk for recurrence by 5.4-fold and 4.6-fold, respectively. No deep vein thrombosis or pulmonary embolism occurred. Dislipidemia and gene mutations of F II are associated with SVT recurrence in patients with VVs. A selection of patients may benefit from anticoagulation in the short term and from VVs intervention in the long term. © The Author(s) 2015.

  14. Avaliação genética de indivíduos e progênies de cupuaçuzeiro no estado do Pará e estimativas de parâmetros genéticos Genetic evaluation of individuals and progenies of Theobroma grandiflorum in the state of Pará and estimates of genetic parameters

    Directory of Open Access Journals (Sweden)

    Rafael Moysés Alves

    2008-09-01

    genetic gain increases from 75.5% to 88.3%, that is to say, from 17 to almost 19 fruits per plant. This reveals a great potential for the commercial cloning of Theobroma grandiflorum. For the characters VBI and VBF high individual narrow sense heritabilities were verified with values ranging from 30% to 54%. This reveals the excellent potential of the recurrent selection to improve the resistance level. It seems enough to consider in the selection just the number of brooms, without being necessary to consider the weight. The correlation between resistance in the fruit and in the inflorescence was high (0.84 indicating some genetic control common to the two characters. Superior progenies were identified for production of fruits and resistance to the broom simultaneously.

  15. Recurrence of anxiety disorders and its predictors

    NARCIS (Netherlands)

    Scholten, Willemijn D.; Batelaan, Neeltje M.; van Balkom, Anton J. L. M.; Penninx, Brenda; Smit, Johannes H.; van Oppen, Patricia

    Background: The chronic course of anxiety disorders and its high burden of disease are partly due to the recurrence of anxiety disorders after remission. However, knowledge about recurrence rates and predictors of recurrence is scarce. This article reports on recurrence rates of anxiety disorders

  16. Recurrent pregnancy loss: current perspectives

    Directory of Open Access Journals (Sweden)

    El Hachem H

    2017-05-01

    Full Text Available Hady El Hachem,1,2 Vincent Crepaux,3 Pascale May-Panloup,4 Philippe Descamps,3 Guillaume Legendre,3 Pierre-Emmanuel Bouet3 1Department of Reproductive Medicine, Ovo Clinic, Montréal, QC, Canada; 2Department of Obstetrics and Gynecology, University of Montreal, Montréal, QC, Canada; 3Department of Obstetrics and Gynecology, Angers University Hopsital, Angers, France; 4Department of Reproductive Biology, Angers University Hospital, Angers, France Abstract: Recurrent pregnancy loss is an important reproductive health issue, affecting 2%–5% of couples. Common established causes include uterine anomalies, antiphospholipid syndrome, hormonal and metabolic disorders, and cytogenetic abnormalities. Other etiologies have been proposed but are still considered controversial, such as chronic endometritis, inherited thrombophilias, luteal phase deficiency, and high sperm DNA fragmentation levels. Over the years, evidence-based treatments such as surgical correction of uterine anomalies or aspirin and heparin for antiphospholipid syndrome have improved the outcomes for couples with recurrent pregnancy loss. However, almost half of the cases remain unexplained and are empirically treated using progesterone supplementation, anticoagulation, and/or immunomodulatory treatments. Regardless of the cause, the long-term prognosis of couples with recurrent pregnancy loss is good, and most eventually achieve a healthy live birth. However, multiple pregnancy losses can have a significant psychological toll on affected couples, and many efforts are being made to improve treatments and decrease the time needed to achieve a successful pregnancy. This article reviews the established and controversial etiologies, and the recommended therapeutic strategies, with a special focus on unexplained recurrent pregnancy losses and the empiric treatments used nowadays. It also discusses the current role of preimplantation genetic testing in the management of recurrent pregnancy

  17. Effect of Polish and foreign purebred Arabian stallions on conformation traits of their progeny participating in shows in the last decade

    Directory of Open Access Journals (Sweden)

    Rafal Czarnecki

    2014-12-01

    Full Text Available Observations confirm correctness of the statement that the best recommendation for a stallion is his progeny. The aim of this study was indicating purebred Arabian stallions passing on to their progeny best conformation traits, assessed in Polish shows, in the past decade, taking into consideration their origin. The analysis included national and foreign stallions used in Polish breeding of purebred Arabian horses, which are fathers of at least 5 heads of progeny. The authors own research proved that progeny of foreign stallions statistically differed significantly from the progeny sired by Polish stallions within all conformation traits tested in shows. In the studied period, the highest final score (91.37 points and note for the trait type (19.11 points was characteristic of the progeny of QR Marc. The best head was passed on by stallions: QR Marc (19.06 points and WH Justice (18.78 points, born in the United States and owned by the European breeders. In the Top Ten of producers passing on a refined head to their progeny, there was only one Polish stallion bred in the Horse Stud Michalw, Ekstern (18.29 points. Progeny with the most correct body structure was sired by foreign stallions, Eden C (18.13 points and QR Marc (18.13 points, and by the Polish stallion, Zlocien (17.95 points. The highest score for the trait legs was obtained by the progeny of the Qatar stallion Gazal Al Shaqab (16.30 points. However, the title of the father of the best movers got an Israeli stallion, Laheeb (18.91 points.

  18. BRCA1 deficiency in skin epidermis leads to selective loss of hair follicle stem cells and their progeny

    Science.gov (United States)

    Sotiropoulou, Panagiota A.; Karambelas, Andrea E.; Debaugnies, Maud; Candi, Aurelie; Bouwman, Peter; Moers, Virginie; Revenco, Tatiana; Rocha, Ana Sofia; Sekiguchi, Kiyotoshi; Jonkers, Jos; Blanpain, Cedric

    2013-01-01

    The accurate maintenance of genomic integrity is essential for tissue homeostasis. Deregulation of this process leads to cancer and aging. BRCA1 is a critical mediator of this process. Here, we performed conditional deletion of Brca1 during epidermal development and found that BRCA1 is specifically required for hair follicle (HF) formation and for development of adult HF stem cells (SCs). Mice deficient for Brca1 in the epidermis are hairless and display a reduced number of HFs that degenerate progressively. Surprisingly, the interfollicular epidermis and the sebaceous glands remain unaffected by Brca1 deletion. Interestingly, HF matrix transient amplifying progenitors present increased DNA damage, p53 stabilization, and caspase-dependent apoptosis compared with the interfollicular and sebaceous progenitors, leading to hyperproliferation, apoptosis, and subsequent depletion of the prospective adult HF SCs. Concomitant deletion of p53 and Brca1 rescues the defect of HF morphogenesis and loss of HF SCs. During adult homeostasis, BRCA1 is dispensable for quiescent bulge SCs, but upon their activation during HF regeneration, Brca1 deletion causes apoptosis and depletion of Brca1-deficient bulge SCs. Our data reveal a major difference in the requirement of BRCA1 between different types of epidermal SCs and progenitors and during the different activation stages of adult HF SCs. PMID:23271346

  19. Comparison of efficiency of distance measurement methodologies in mango (Mangifera indica) progenies based on physicochemical descriptors.

    Science.gov (United States)

    Alves, E O S; Cerqueira-Silva, C B M; Souza, A M; Santos, C A F; Lima Neto, F P; Corrêa, R X

    2012-03-14

    We investigated seven distance measures in a set of observations of physicochemical variables of mango (Mangifera indica) submitted to multivariate analyses (distance, projection and grouping). To estimate the distance measurements, five mango progeny (total of 25 genotypes) were analyzed, using six fruit physicochemical descriptors (fruit weight, equatorial diameter, longitudinal diameter, total soluble solids in °Brix, total titratable acidity, and pH). The distance measurements were compared by the Spearman correlation test, projection in two-dimensional space and grouping efficiency. The Spearman correlation coefficients between the seven distance measurements were, except for the Mahalanobis' generalized distance (0.41 ≤ rs ≤ 0.63), high and significant (rs ≥ 0.91; P < 0.001). Regardless of the origin of the distance matrix, the unweighted pair group method with arithmetic mean grouping method proved to be the most adequate. The various distance measurements and grouping methods gave different values for distortion (-116.5 ≤ D ≤ 74.5), cophenetic correlation (0.26 ≤ rc ≤ 0.76) and stress (-1.9 ≤ S ≤ 58.9). Choice of distance measurement and analysis methods influence the.

  20. [Hypoploid groups and their cytogenetic analysis in the progenies between Brassica napus and Orychophragmus violaceus].

    Science.gov (United States)

    Wu, Jian-Guo; Shi, Chun-Hai; Lin, Xing-Wu; Li, Zai-Yun; Fu, Ting-Dong

    2004-11-01

    Several groups of hypoploids were characterized in pentaploid (p) progenies of B. napus and O. violaceus. Cytological characterization of the P3 population revealed allosubstitution of one pair of B. napus chromosomes with O. violaceus chromosomes. This population had normal fertility and had three kinds of somatic cells with 36-38 chromosomes. Its somatic cells and pollen mother cells (PMC) mainly had 38 chromosomes. From a P4 population, three monosomic plants with 37 chromosomes were identified with differing morphology and fertility. One plant which grew vigorously was composed of various types of somatic cell and PMC, in which the cells with 37 chromosomes were observed mainly. The unpaired chromosome was small and with no negative effects on plant development. In the offsprings of the aneuploids (P3) with 41-44 chromosomes, there are four types of plants with 29-32 chromosomes. Except the plant with 29 chromosomes, others which appeared abnormal meiosis behavior and poor pollen fertility are mixoploid. The origin of these hypoploids and their prospects of application have been discussed.

  1. Regional deposition of thoron progeny in models of the human tracheobronchial tree

    Energy Technology Data Exchange (ETDEWEB)

    Smith, S.M.; Cheng, Yung-Sung; Yeh, Hsu-Chi

    1995-12-01

    Models of the human tracheobronchial tree have been used to determine total and regional aerosol deposition of inhaled particles. Particle sizes measured in these studies have all been > 40 nm in diameter. The deposition of aerosols < 40 nm in diameter has not been measured. Particles in the ultrafine aerosol size range include some combustion aerosols and indoor radon progeny. Also, the influence of reduced lung size and airflow rates on particle deposition in young children has not been determined. With their smaller lung size and smaller minute volumes, children may be at increased risk from ultrafine pollutants. In order to accurately determine dose of inhaled aerosols, the effects of particle size, minute volume, and age at exposure must be quantified. The purpose of this study was to determine the deposition efficiency of ultrafine aerosols smaller than 40 nm in diameter in models of the human tracheobronchia tree. This study demonstrates that the deposition efficiency of aerosols in the model of the child`s tracheobronchial tree may be slightly higher than in the adult models.

  2. [Transgenerational impact of chemotherapy: Would the father exposure impact the health of future progeny?

    Science.gov (United States)

    Tremblay, A; Beaud, H; Delbès, G

    2017-11-01

    The number of cancer survivors is increasing and their quality of life is becoming a major public health issue. Cancer treatments reduce men's reproductive health by targeting spermatogenesis. Ultimately, DNA, chromatin and the epigenome of spermatozoa can be altered in cancer survivors. Knowing whether the history of cancer and the treatments received can have consequences on the health of their offspring is therefore a fundamental question for these patients. This review gathers the experimental and epidemiological evidences of the effects observed on the direct descendants and on several generations, and draws up the state of knowledge on the mechanisms potentially involved. Experimental data describe inter- and transgenerational effects of paternal exposure depending on the type of treatment, dose and time of exposure. In the human population, the analysis of the effects specifically due to chemotherapy is still limited because they are often combined with irradiation treatments. However, it appears that chemotherapy agents affect the birth rate but do not have a significant impact on the health of the children born. Nevertheless, the demonstration of modifications of the sperm epigenome in cancer survivors, even after a period of remission, as well as changes in the sperm of the progeny in animal models, suggests a possible transgenerational transmission that remains to be studied in the human population. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  3. BABA-primed defense responses to Phytophthora infestans in the next vegetative progeny of potato

    Directory of Open Access Journals (Sweden)

    Jolanta eFloryszak-Wieczorek

    2015-10-01

    Full Text Available The transcript of the PR1 gene accumulation as an informative marker of systemic acquired resistance (SAR was analyzed in β-aminobutyric acid (BABA primed potato in the short-lasting (3 days and long-lasting (28 days time periods after induction and in the vegetative descendants of primed plants derived from tubers and from in vitro seedlings. BABA pretreatment resulted either in minimal or no PR1 gene expression, but sequential treatment with BABA followed by virulent P. infestans provided data on the imprint of post-stress information and its duration until fertilization, in the form of an enhanced PR1 transcript accumulation and a transient increase of basal resistance to the late blight disease. The primed state for defense of the susceptible potato cultivar was transmitted to its vegetative progeny as a potentiated PR1 mRNA accumulation following challenge inoculation. However,variation was observed between vegetative accessions of the BABA-primed potato genotype in responsiveness to disease. In contrast to plants derived from tubers, potato propagated through in vitro seedlings largely lost inducible resistance traits, although itretained primed PR1 gene expression.

  4. Maternal protein restriction early in rat pregnancy alters brain development in the progeny.

    Science.gov (United States)

    Gressens, P; Muaku, S M; Besse, L; Nsegbe, E; Gallego, J; Delpech, B; Gaultier, C; Evrard, P; Ketelslegers, J M; Maiter, D

    1997-10-20

    We assessed the effects of a dietary protein restriction (5% vs. 20% casein in diet) initiated at conception and imposed during the first 2 weeks of rat gestation on postnatal brain development. At the end of the malnutrition period, protein-restricted animals exhibited significantly smaller fetal body weight and brain cortical thickness than controls. At birth and thereafter, body weight was normalized in the progeny. Similarly, brain weight and cytoarchitecture were normal in postnatal animals. In contrast, we observed, during the first 2 postnatal weeks, several abnormalities of brain development which affected all the studied areas for most of the studied parameters: (i) delayed astrocytogenesis as shown by a reduced GFAP staining; (ii) delayed production of hyaluronan in the extracellular matrix studied with binding of biotinylated hyaluronectin; (iii) abnormal neuronal differentiation as shown by reduced expression of MAP-5 and increased expression of MAP-1; (iv) abnormal synaptogenesis as shown by the increased expression of synaptophysin in the basal ganglia; (v) decreased programmed cell death. In adult prenatally protein-restricted animals, all the above parameters were normalized excepted MAP-1 labeling which remained high. In addition, we observed slight alterations of the ventilatory response to hypoxia in adult animals. The present study demonstrates that early protein malnutrition during embryonic development induces multiple, transient alterations of brain development. However, the almost complete normalization in adults of brain architecture and differentiation as well as our physiological data strongly suggest a remarkable plasticity of the developing brain following an early aggression.

  5. Detection of Progeny Immune Responses after Intravenous Administration of DNA Vaccine to Pregnant Mice

    Directory of Open Access Journals (Sweden)

    Xin Ke-Qin

    2002-01-01

    Full Text Available A number of factors influence the development of tolerance, including the nature, concentration and mode of antigen presentation to the immune system, as well as the age of the host. The studies were conducted to determine whether immunizing pregnant mice with liposome-encapsulated DNA vaccines had an effect on the immune status of their offspring. Two different plasmids (encoding antigens from HIV-1 and influenza virus were administered intravenously to pregnant mice. At 9.5 days post conception with cationic liposomes, injected plasmid was present in the tissues of the fetus, consistent with trans-placental transfer. When the offspring of vaccinated dams were immunized with DNA vaccine, they mounted stronger antigen-specific immune responses than controls and were protected against challenge by homologous influenza virus after vaccination. Moreover, such immune responses were strong in the offspring of mothers injected with DNA plasmid 9.5 days after coitus. These results suggest that DNA vaccinated mothers confer the antigen-specific immunity to their progeny. Here we describe the methods in detail as they relate to our previously published work.

  6. A study of maternal lymphoid organs and the progeny following treatment with immunomodulating agents during pregnancy.

    Science.gov (United States)

    Scott, J R; Feldbush, T L; Covault, J M

    1977-01-01

    Whether differences in foetoplacental weight and post-implantation mortality in rodents are secondary to heterosis and inbreeding depression or antigenic differences between mother and foetus has been a continuing controversy. To determine whether non-specific depression or stimulation of the maternal immune system affects the success of the foetoplacental allograft, groups of virgin Fischer (Ag-B1) females of similar age and weight mated with DA (Ag-B4) males were treated with daily intraperitoneal injections of: (a) saline, (b) methylprednisolone (MP), 1-0 mg/kg, (c) cyclophosphamide (CY), 3.0 mg/kg, or (d) azathioprine (AZ), 3.0 mg/kg; or they were injected intraperitoneally on the fifth day of gestation with: (a) B. pertussis, 1.0 ml, (b) C. parvum, 0.2 ml, or (c) BCG, 0.1 ml. None of the immunostimulating agents were detrimental to the progeny, but the immunosupprissive drugs caused an increased percentage of foetal deaths and foetoplacental growth retardation. The reduced foetal and placental size induced by CY or AZ could be partially blocked by simultaneous maternal treatment with BCG. Analysis of mean maternal weight gain, spleen weight assays, changes in the lymph nodes draining the uterus and comparison of data from non-pregnant animals and syngeneic pregnancies treated with these agents suggest that immunosuppressive drugs reduce foetal survival rates and produce foetoplacental growth retardation via a combination of immunological and cytotoxic mechanisms. PMID:342160

  7. Norethindrone-induced masculinization and progeny testing in guppy, Poecilia reticulata (Peters 1859).

    Science.gov (United States)

    Basavaraja, N; Chandrashekhara, B H; Ahamad, Rather Mansoor

    2014-03-01

    Norethindrone(NE) was evaluated for its efficacy on alteration of sex ratio of P. reticulata. Either the young fry or the brooders and the resultant fry were fed a commercial diet incorporated with NE at 0, 25, 50, 75 and 100 mg kg(-1) diet (ppm) for 30-40 d in rectangular glass aquaria; this was followed by 40-60 d rearing on NE-free diet in out-door concrete tanks. In general, the androgen treatment altered sex ratio, leading to the production of a dose dependent increase in the percentage of males. The oral administration of the steroid at 75 ppm for 40 d or 100 ppmfor 30 or 40 d to first feeding fry, yielded 100% males. On the other hand, NE administration to brooders before parturition and the resultant fry also produced an all-male population of guppy. The sex ratio of the untreated control was almost 1:1. The survival of fish in all the trials was high, ranging between 67 and 100%. Mating masculinized males ("XX" male) with normal female resulted in an all-female progeny, while crossing normal male (XY) from treatment groups with normal female sired normal sex ratio (1:1), elucidating XX-XY sex determination system in the guppy.

  8. Fate of neuroblast progeny during postembryonic development of mushroom bodies in the house cricket, Acheta domesticus.

    Science.gov (United States)

    Cayre, M; Malaterre, J; Charpin, P; Strambi, C; Strambi, A

    2000-03-01

    Mushroom bodies represent the main sensory integrative center of the insect brain and probably play a major role in the adaptation of behavioral responses to the environment. Taking into account the continuous neurogenesis of cricket mushroom bodies, we investigated ontogenesis of this brain structure. Using BrdU labeling, we examined the fate of neuroblast progeny during the postembryonic development. Preimaginal Kenyon cells survived throughout larval and imaginal moults and persisted during adulthood. Our results indicate that the location of labelled Kenyon cells in the cortex of the adult cricket mainly depends upon the period when they were produced during development. The present data demonstrate that cricket mushroom bodies grow from the inside out and that, at any developmental stage, the center of the cortex contains the youngest Kenyon cells. This study also allowed us to observe the occurrence of quiescent neuroblasts. Kenyon cell death during postembryonic and adult life seems to be reduced. Although preimaginal Kenyon cells largely contribute to adult mushroom body structure, a permanent remodeling of the mushroom body occurs throughout the whole insect life due to the persistence of neurogenesis in the house cricket. Further studies are needed to understand the functional significance of these findings.

  9. [Epithelial ovarian carcinomas: what to do after recurrences?].

    Science.gov (United States)

    Bobin, Jean-Yves

    2004-10-31

    Follow up for epithelial ovarian carcinomas is not standardized. A close follow-up every three months during three years, and then twice a year during five years seems to be acceptable. Recurrences after primary treatment of ovarian cancer have very bad prognosis. The major treatment must be chemotherapies. Salvage cytoreduction surgery is effective only in very selected cases.

  10. Immunomodulatory therapy in refractory/recurrent ovarian cancer

    Directory of Open Access Journals (Sweden)

    Chao-Yu Chen

    2015-04-01

    Conclusion: IMT alone or add-on to palliative/salvage chemotherapy for refractory/recurrent EOC achieves a substantial disease stabilizing rate without severe toxicity, which might be a potential option in selected patients. The ALC 1 month after IMT could be an early indicator to disease stabilization.

  11. Influence of antioxidant compounds, total sugars and genetic background on the chilling injury susceptibility of a non-melting peach (Prunus persica (L.) Batsch) progeny.

    Science.gov (United States)

    Abidi, Walid; Cantín, Celia M; Jiménez, Sergio; Giménez, Rosa; Moreno, María Ángeles; Gogorcena, Yolanda

    2015-01-01

    To identify genotypes with good organoleptic properties, antioxidant-rich content and low susceptibility to chilling injury (CI), fruits from 130 peach cultivars were studied over three consecutive years. Pomological traits, l-ascorbic acid, flavonoids, total phenolics, relative antioxidant capacity (RAC) and sugars were determined. Major symptoms of CI developed at 5 °C, such as leatheriness, flesh browning, bleeding and loss of flavor, were evaluated. The population exhibited wide phenotypic variation in agronomic and biochemical traits. Six genotypes with high total phenolics, RAC, flavonoids and total sugars were selected. The progeny also showed variability for all evaluated CI symptoms, and 16 genotypes showed considerably lower susceptibility to CI. After 2 weeks of cold storage, leatheriness and bleeding were the main CI symptoms observed, whereas flesh browning was predominant after 4 weeks. It was possible to find varieties with high phenolic concentration and relatively low or intermediate CI susceptibility (22, 33, 68, 80, 81, 96 and 120). However, the correlations observed between CI and phenolic contents highlight their potential influence on susceptibility to internal browning. This relationship should be considered in the current breeding programs to select cultivars with high bioactive compound contents, health-enhancing properties and good postharvest performance. © 2014 Society of Chemical Industry.

  12. Genetic profiling to predict recurrence of early cervical cancer.

    Science.gov (United States)

    Lee, Yoo-Young; Kim, Tae-Joong; Kim, Ji-Young; Choi, Chel Hun; Do, In-Gu; Song, Sang Yong; Sohn, Insuk; Jung, Sin-Ho; Bae, Duk-Soo; Lee, Jeong-Won; Kim, Byoung-Gie

    2013-12-01

    Recurrence is the major cause of death in early cervical cancer. We compared the prediction powers for disease recurrence between the gene set prognostic model and the clinical prognostic model. A gene set model to predict disease free survival was developed using the cDNA-mediated annealing, selection, extension, and ligation (DASL) assay data set from a cohort of early cervical cancer patients who had been treated with radical surgery with or without adjuvant therapy. A clinical prediction model was also developed using the same cohort, and the ability of predicting recurrence from each model was compared. Adequate DASL assay profiles were obtained from 300 patients, and we selected 12 genes for the gene set model. When patients were categorized as having a low or high risk by the prognostic score, the Kaplan-Meier curve showed significantly different recurrence rates between the two groups. The clinical model was developed using FIGO stage and post-surgical pathological findings. In multivariate Cox regression analysis of prognostic models, the gene set prognostic model showed a higher hazard ratio than that of the clinical prognostic model. The genetic quantitative approach may be better in predicting recurrence in early cervical cancer patients. © 2013 Elsevier Inc. All rights reserved.

  13. Phase I trial of palbociclib, a selective cyclin dependent kinase 4/6 inhibitor, in combination with cetuximab in patients with recurrent/metastatic head and neck squamous cell carcinoma.

    Science.gov (United States)

    Michel, Loren; Ley, Jessica; Wildes, Tanya M; Schaffer, András; Robinson, Anthony; Chun, Se-Eun; Lee, Wooin; Lewis, James; Trinkaus, Kathryn; Adkins, Douglas

    2016-07-01

    To test the safety of the CDK4/6 inhibitor palbociclib with cetuximab in patients with recurrent/metastatic head and neck squamous cell carcinoma (HNSCC). A phase I trial using 3+3 design was performed to determine the dose limiting toxicity (DLT) and maximum tolerated dose (MTD) of palbociclib with standard dose weekly cetuximab. Palbociclib was administered orally days 1-21 every 28days: dose level 1 (100mg/d) and 2 (125mg/d; approved monotherapy dose). Pharmacokinetic assessments were performed on cycle 2, day 15. Cyclin D1, p16(INK4a), and Rb protein expression were measured on pre-treatment tumor. Tumor response was assessed using RECIST1.1. Nine patients (five p16(INK4a) negative; four positive) were enrolled across dose levels 1 (n=3) and 2 (n=6) and none experienced a DLT. A MTD of palbociclib was not reached. Myelosuppression was the most common adverse event. Six of nine patients had cetuximab-resistant and 4/9 had platin-resistant disease. Disease control (DC) occurred in 89%, including partial response (PR) in two (22%) and stable disease in six (67%) patients. PRs occurred in p16(INK4a) negative HNSCC. Five patients (56%) had measurable decreases in tumor target lesions. In cetuximab-resistant HNSCC, best tumor response was PR in 1 and DC in 5 and median TTP was 112days (range: 28-168). In platin-resistant HNSCC, best tumor response: PR in 1, DC in 3 and median TTP was 112days (range: 28-112). The Cmax and AUC0-24h appeared comparable in patients receiving 125 vs 100mg dose of palbociclib. This trial, the first to evaluate a CDK4/6 inhibitor in HNSCC, determined that palbociclib 125mg/day on days 1-21 every 28days with cetuximab was safe. Tumor responses were observed, even in cetuximab- or platin-resistant disease. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. RECURRENT NOVAE IN M31

    Energy Technology Data Exchange (ETDEWEB)

    Shafter, A. W. [Department of Astronomy, San Diego State University, San Diego, CA 92182 (United States); Henze, M. [European Space Astronomy Centre, P.O. Box 78, E-28692 Villanueva de la Cañada, Madrid (Spain); Rector, T. A. [Department of Physics and Astronomy, University of Alaska Anchorage, 3211 Providence Dr., Anchorage, AK 99508 (United States); Schweizer, F. [Carnegie Observatories, 813 Santa Barbara St., Pasadena, CA 91101 (United States); Hornoch, K. [Astronomical Institute, Academy of Sciences, CZ-251 65 Ondřejov (Czech Republic); Orio, M. [Astronomical Observatory of Padova (INAF), I-35122 Padova (Italy); Pietsch, W. [Max Planck Institute for Extraterrestrial Physics, P.O. Box 1312, Giessenbachstr., D-85741, Garching (Germany); Darnley, M. J.; Williams, S. C.; Bode, M. F. [Astrophysics Research Institute, Liverpool John Moores University, Liverpool L3 5RF (United Kingdom); Bryan, J., E-mail: aws@nova.sdsu.edu [McDonald Observatory, Austin, TX 78712 (United States)

    2015-02-01

    The reported positions of 964 suspected nova eruptions in M31 recorded through the end of calendar year 2013 have been compared in order to identify recurrent nova (RN) candidates. To pass the initial screen and qualify as a RN candidate, two or more eruptions were required to be coincident within 0.′1, although this criterion was relaxed to 0.′15 for novae discovered on early photographic patrols. A total of 118 eruptions from 51 potential RN systems satisfied the screening criterion. To determine what fraction of these novae are indeed recurrent, the original plates and published images of the relevant eruptions have been carefully compared. This procedure has resulted in the elimination of 27 of the 51 progenitor candidates (61 eruptions) from further consideration as RNe, with another 8 systems (17 eruptions) deemed unlikely to be recurrent. Of the remaining 16 systems, 12 candidates (32 eruptions) were judged to be RNe, with an additional 4 systems (8 eruptions) being possibly recurrent. It is estimated that ∼4% of the nova eruptions seen in M31 over the past century are associated with RNe. A Monte Carlo analysis shows that the discovery efficiency for RNe may be as low as 10% that for novae in general, suggesting that as many as one in three nova eruptions observed in M31 arise from progenitor systems having recurrence times ≲100 yr. For plausible system parameters, it appears unlikely that RNe can provide a significant channel for the production of Type Ia supernovae.

  15. Efficacy of Enrofloxacin in the Treatment of Recurrent Pyoderma in Dogs

    Directory of Open Access Journals (Sweden)

    B. Sudhakara Reddy

    2014-07-01

    Full Text Available Dogs with a history of more than three episodes of skin infections in a period of one year were selected for a study on recurrent pyoderma. Oral enrofloxacin along with appropriate simultaneous medication for the underlying associated conditions were chosen as therapy for recurrent pyoderma in dogs. Response to therapy was excellent in all the cases. Improvement was noticed by 12 to 20 days and 20 to 26 days in recurrent superficial and deep pyoderma respectively. Relapse occurred in one dog by 45 days due to re-introduction of allergic food. Enrofloxacin proved to be an effective, safe and convenient antibiotic for the treatment of recurrent pyoderma in dogs.

  16. Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration

    Science.gov (United States)

    El Khatib, Moustafa M.; Ohmine, Seiga; Jacobus, Egon J.; Tonne, Jason M.; Morsy, Salma G.; Holditch, Sara J.; Schreiber, Claire A.; Uetsuka, Koji; Fusaki, Noemi; Wigle, Dennis A.; Terzic, Andre; Kudva, Yogish C.

    2016-01-01

    Human induced pluripotent stem cells (iPSCs) and derived progeny provide invaluable regenerative platforms, yet their clinical translation has been compromised by their biosafety concern. Here, we assessed the safety of transplanting patient-derived iPSC-generated pancreatic endoderm/progenitor cells. Transplantation of progenitors from iPSCs reprogrammed by lentiviral vectors (LV-iPSCs) led to the formation of invasive teratocarcinoma-like tumors in more than 90% of immunodeficient mice. Moreover, removal of primary tumors from LV-iPSC progeny-transplanted hosts generated secondary and metastatic tumors. Combined transgene-free (TGF) reprogramming and elimination of residual pluripotent cells by enzymatic dissociation ensured tumor-free transplantation, ultimately enabling regeneration of type 1 diabetes-specific human islet structures in vivo. The incidence of tumor formation in TGF-iPSCs was titratable, depending on the oncogenic load, with reintegration of the cMYC expressing vector abolishing tumor-free transplantation. Thus, transgene-free cMYC-independent reprogramming and elimination of residual pluripotent cells are mandatory steps in achieving transplantation of iPSC progeny for customized and safe islet regeneration in vivo. Significance Pluripotent stem cell therapy for diabetes relies on the safety as well as the quality of derived insulin-producing cells. Data from this study highlight prominent tumorigenic risks of induced pluripotent stem cell (iPSC) products, especially when reprogrammed with integrating vectors. Two major underlying mechanisms in iPSC tumorigenicity are residual pluripotent cells and cMYC overload by vector integration. This study also demonstrated that combined transgene-free reprogramming and enzymatic dissociation allows teratoma-free transplantation of iPSC progeny in the mouse model in testing the tumorigenicity of iPSC products. Further safety assessment and improvement in iPSC specification into a mature β cell phenotype

  17. Study of the atmospheric chemistry of radon progeny in laboratory and real indoor atmospheres. Final project report

    Energy Technology Data Exchange (ETDEWEB)

    Hopke, P.K.

    1996-09-01

    This report completes Clarkson University`s study of the chemical and physical behavior of the {sup 218}Po atom immediately following its formation by the alpha decay of radon. Because small changes in size for activity in the sub-10 nm size range result in large changes in the delivered dose per unit exposure, this behavior must be understood if the exposure to radon progeny and it dose to the cells in the respiratory tract are to be fully assessed. In order to pursue this general goal, two areas of radon progeny behavior are being pursued; laboratory studies under controlled conditions to better understand the fundamental physical and chemical processes that affect the progeny`s atmospheric behavior and studies in actual indoor environments to develop a better assessment of the exposure of the occupants of that space to the size and concentration of the indoor radioactive aerosol. Thus, two sets of specific goals have been established for this project. The specific tasks of the controlled laboratory studies are (1) Determine the formation rates of {circ}OH radicals formed by the radiolysis of air following radon decay; (2) Examine the formation of particles by the radiolytic oxidation of substances like SO{sub 2}, ethylene, and H{sub 2}S to lower vapor pressure compounds and determine the role of gas phase additives such as H{sub 2}O and NH{sub 3} in determining the particle size; (3) Measure the rate of ion-induced nucleation using a thermal diffusion cloud chamber, and (4) Measure the neutralization rate of {sup 218}PoO{sub x}{sup +} in O{sub 2} at low radon concentrations.

  18. Proviral Progeny of Heterodimeric Virions Reveal a High Crossover Rate for Human Immunodeficiency Virus Type 2▿

    OpenAIRE

    Mukherjee, Sayandip; Lee, Hui-Ling Rose; Ron, Yacov; Dougherty, Joseph P.

    2006-01-01

    Human immunodeficiency virus type 1 (HIV-1), the causative agent of AIDS in humans, exhibits a very high rate of recombination. Bearing in mind the significant epidemiological and clinical contrast between HIV-2 and HIV-1 as well as the critical role that recombination plays in viral evolution, we examined the nature of HIV-2 recombination. Towards this end, a strategy was devised to measure the rate of crossover of HIV-2 by evaluating recombinant progeny produced exclusively by heterodimeric...

  19. Maternal Exposure to Cadmium and Manganese Impairs Reproduction and Progeny Fitness in the Sea Urchin Paracentrotus lividus

    Science.gov (United States)

    Migliaccio, Oriana; Castellano, Immacolata; Cirino, Paola; Romano, Giovanna; Palumbo, Anna

    2015-01-01

    Metal contamination represents one of the major sources of pollution in marine environments. In this study we investigated the short-term effects of ecologically relevant cadmium and manganese concentrations (10-6 and 3.6 x 10-5 M, respectively) on females of the sea urchin Paracentrotus lividus and their progeny, reared in the absence or presence of the metal. Cadmium is a well-known heavy metal, whereas manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. The effects of these agents were examined on both P. lividus adults and their offspring following reproductive state, morphology of embryos, nitric oxide (NO) production and differential gene expression. Here, we demonstrated that both metals differentially impaired the fertilization processes of the treated female sea urchins, causing modifications in the reproductive state and also affecting NO production in the ovaries. A detailed analysis of the progeny showed a high percentage of abnormal embryos, associated to an increase in the endogenous NO levels and variations in the transcriptional expression of several genes involved in stress response, skeletogenesis, detoxification, multi drug efflux processes and NO production. Moreover, we found significant differences in the progeny from females exposed to metals and reared in metal-containing sea water compared to embryos reared in non-contaminated sea water. Overall, these results greatly expanded previous studies on the toxic effects of metals on P. lividus and provided new insights into the molecular events induced in the progeny of sea urchins exposed to metals. PMID:26125595

  20. Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration.

    Science.gov (United States)

    El Khatib, Moustafa M; Ohmine, Seiga; Jacobus, Egon J; Tonne, Jason M; Morsy, Salma G; Holditch, Sara J; Schreiber, Claire A; Uetsuka, Koji; Fusaki, Noemi; Wigle, Dennis A; Terzic, Andre; Kudva, Yogish C; Ikeda, Yasuhiro

    2016-05-01

    Human induced pluripotent stem cells (iPSCs) and derived progeny provide invaluable regenerative platforms, yet their clinical translation has been compromised by their biosafety concern. Here, we assessed the safety of transplanting patient-derived iPSC-generated pancreatic endoderm/progenitor cells. Transplantation of progenitors from iPSCs reprogrammed by lentiviral vectors (LV-iPSCs) led to the formation of invasive teratocarcinoma-like tumors in more than 90% of immunodeficient mice. Moreover, removal of primary tumors from LV-iPSC progeny-transplanted hosts generated secondary and metastatic tumors. Combined transgene-free (TGF) reprogramming and elimination of residual pluripotent cells by enzymatic dissociation ensured tumor-free transplantation, ultimately enabling regeneration of type 1 diabetes-specific human islet structures in vivo. The incidence of tumor formation in TGF-iPSCs was titratable, depending on the oncogenic load, with reintegration of the cMYC expressing vector abolishing tumor-free transplantation. Thus, transgene-free cMYC-independent reprogramming and elimination of residual pluripotent cells are mandatory steps in achieving transplantation of iPSC progeny for customized and safe islet regeneration in vivo. Pluripotent stem cell therapy for diabetes relies on the safety as well as the quality of derived insulin-producing cells. Data from this study highlight prominent tumorigenic risks of induced pluripotent stem cell (iPSC) products, especially when reprogrammed with integrating vectors. Two major underlying mechanisms in iPSC tumorigenicity are residual pluripotent cells and cMYC overload by vector integration. This study also demonstrated that combined transgene-free reprogramming and enzymatic dissociation allows teratoma-free transplantation of iPSC progeny in the mouse model in testing the tumorigenicity of iPSC products. Further safety assessment and improvement in iPSC specification into a mature β cell phenotype would lead to

  1. Maternal Exposure to Cadmium and Manganese Impairs Reproduction and Progeny Fitness in the Sea Urchin Paracentrotus lividus.

    Directory of Open Access Journals (Sweden)

    Oriana Migliaccio

    Full Text Available Metal contamination represents one of the major sources of pollution in marine environments. In this study we investigated the short-term effects of ecologically relevant cadmium and manganese concentrations (10(-6 and 3.6 x 10(-5 M, respectively on females of the sea urchin Paracentrotus lividus and their progeny, reared in the absence or presence of the metal. Cadmium is a well-known heavy metal, whereas manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. The effects of these agents were examined on both P. lividus adults and their offspring following reproductive state, morphology of embryos, nitric oxide (NO production and differential gene expression. Here, we demonstrated that both metals differentially impaired the fertilization processes of the treated female sea urchins, causing modifications in the reproductive state and also affecting NO production in the ovaries. A detailed analysis of the progeny showed a high percentage of abnormal embryos, associated to an increase in the endogenous NO levels and variations in the transcriptional expression of several genes involved in stress response, skeletogenesis, detoxification, multi drug efflux processes and NO production. Moreover, we found significant differences in the progeny from females exposed to metals and reared in metal-containing sea water compared to embryos reared in non-contaminated sea water. Overall, these results greatly expanded previous studies on the toxic effects of metals on P. lividus and provided new insights into the molecular events induced in the progeny of sea urchins exposed to metals.

  2. Maternal Exposure to Cadmium and Manganese Impairs Reproduction and Progeny Fitness in the Sea Urchin Paracentrotus lividus.

    Science.gov (United States)

    Migliaccio, Oriana; Castellano, Immacolata; Cirino, Paola; Romano, Giovanna; Palumbo, Anna

    2015-01-01

    Metal contamination represents one of the major sources of pollution in marine environments. In this study we investigated the short-term effects of ecologically relevant cadmium and manganese concentrations (10(-6) and 3.6 x 10(-5) M, respectively) on females of the sea urchin Paracentrotus lividus and their progeny, reared in the absence or presence of the metal. Cadmium is a well-known heavy metal, whereas manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. The effects of these agents were examined on both P. lividus adults and their offspring following reproductive state, morphology of embryos, nitric oxide (NO) production and differential gene expression. Here, we demonstrated that both metals differentially impaired the fertilization processes of the treated female sea urchins, causing modifications in the reproductive state and also affecting NO production in the ovaries. A detailed analysis of the progeny showed a high percentage of abnormal embryos, associated to an increase in the endogenous NO levels and variations in the transcriptional expression of several genes involved in stress response, skeletogenesis, detoxification, multi drug efflux processes and NO production. Moreover, we found significant differences in the progeny from females exposed to metals and reared in metal-containing sea water compared to embryos reared in non-contaminated sea water. Overall, these results greatly expanded previous studies on the toxic effects of metals on P. lividus and provided new insights into the molecular events induced in the progeny of sea urchins exposed to metals.

  3. Breast Recurrent Hydatid Cyst Disease.

    Science.gov (United States)

    Temiz, Ayetullah; Albayrak, Yavuz; Akalp, Sevilay Özmen; Yalçın, Ahmet; Albayrak, Ayşe

    2017-01-01

    Introduction: Hydatid cyst is a zoonotic disease seen in endemic areas. It is an important health problem in our country and especially in our Eastern and South-eastern Anatolia Region. Involvement of isolated breast tissue is very rare in hydatid cystdisease. As far as we know, isolated recurrent breast cyst hydatidosis has not been found in the literature. We aimed to present the case of a patient who was diagnosed with recurrent isolated cyst hydatid in the same breast, operated on because of hydatid cyst in the right breast 9 years ago. A hydatid cyst should be considered, particularly in endemic regions, in the differential diagnosis of cystic masses of the breast. If the patient has previously undergone surgery for breast cyst hydatid disease, recurrent cyst hydatid disease should be considered at the differential diagnosis. Celsius.

  4. Retreatment of recurrent nasopharyngeal carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    McNeese, M.D.; Fletcher, G.H.

    1981-01-01

    Thirty patients with recurrent primary nasopharyngeal carcinoma were reirradiated between 1949 and 1976. Twenty eventually demonstrated recurrence at or near the primary site, involving the nasopharynx in 4 and the central nervous system in 16. Long-term palliation was often achieved, and there were no severe complications except for one patient who died of necrosis of the base of the skull. The most frequent problems were hearing loss and trismus; necrosis of the nasopharynx was seen in only 2 patients. With therapy in the range of 18-25 MeV, significant palliation and an occational cure can be achieved without excessive risk. Recurrent disease involving the skull may be controlled for several years using current techniques.

  5. Hypoxia Treatment of Callosobruchus maculatus Females and Its Effects on Reproductive Output and Development of Progeny Following Exposure.

    Science.gov (United States)

    Yan, Yan; Williams, Scott B; Baributsa, Dieudonne; Murdock, Larry L

    2016-06-17

    Modified atmospheres present a residue-free alternative to fumigants for controlling postharvest pests of grain during storage. How sub-lethal applications of this method affects the reproductive fitness of target pests, however, is still not fully understood. We examined how low levels of ambient oxygen influence the reproduction of the female cowpea bruchid (Callosobruchus maculatus), a pest of cowpea. We used three low-oxygen atmospheres-2%, 5% and 10% (v/v) oxygen-and observed their effects on: (1) the number of eggs laid by bruchids compared to insects held in normoxic (~20% oxygen) conditions; (2) the total number of eggs laid; and (3) the number of progeny that reached maturity. Low oxygen did not significantly affect the number of eggs laid during 48 or 72 h of exposure, but 2% and 5% oxygen did negatively affected total egg production. Increasing the exposure time from 48 to 72 h further depressed lifetime reproductive output. Maternal and egg exposure to hypoxia reduced the number of progeny that reached adulthood. Lower adult emergence was observed from eggs laid under low oxygen and longer exposure times. These data demonstrate that hermetic conditions depress the egg-laying behavior of cowpea bruchids and the successful development of their progeny.

  6. Hypoxia Treatment of Callosobruchus maculatus Females and Its Effects on Reproductive Output and Development of Progeny Following Exposure

    Directory of Open Access Journals (Sweden)

    Yan Yan

    2016-06-01

    Full Text Available Modified atmospheres present a residue-free alternative to fumigants for controlling postharvest pests of grain during storage. How sub-lethal applications of this method affects the reproductive fitness of target pests, however, is still not fully understood. We examined how low levels of ambient oxygen influence the reproduction of the female cowpea bruchid (Callosobruchus maculatus, a pest of cowpea. We used three low-oxygen atmospheres—2%, 5% and 10% (v/v oxygen—and observed their effects on: (1 the number of eggs laid by bruchids compared to insects held in normoxic (~20% oxygen conditions; (2 the total number of eggs laid; and (3 the number of progeny that reached maturity. Low oxygen did not significantly affect the number of eggs laid during 48 or 72 h of exposure, but 2% and 5% oxygen did negatively affected total egg production. Increasing the exposure time from 48 to 72 h further depressed lifetime reproductive output. Maternal and egg exposure to hypoxia reduced the number of progeny that reached adulthood. Lower adult emergence was observed from eggs laid under low oxygen and longer exposure times. These data demonstrate that hermetic conditions depress the egg-laying behavior of cowpea bruchids and the successful development of their progeny.

  7. Increased frequency of spontaneous neoplastic transformation in progeny of bystander cells from cultures exposed to densely ionizing radiation.

    Directory of Open Access Journals (Sweden)

    Manuela Buonanno

    Full Text Available An increased risk of carcinogenesis caused by exposure to space radiation during prolonged space travel is a limiting factor for human space exploration. Typically, astronauts are exposed to low fluences of ionizing particles that target only a few cells in a tissue at any one time. The propagation of stressful effects from irradiated to neighboring bystander cells and their transmission to progeny cells would be of importance in estimates of the health risks of exposure to space radiation. With relevance to the risk of carcinogenesis, we investigated, in model C3H 10T½ mouse embryo fibroblasts (MEFs, modulation of the spontaneous frequency of neoplastic transformation in the progeny of bystander MEFs that had been in co-culture 10 population doublings earlier with MEFs exposed to moderate doses of densely ionizing iron ions (1 GeV/nucleon or sparsely ionizing protons (1 GeV. An increase (P<0.05 in neoplastic transformation frequency, likely mediated by intercellular communication through gap junctions, was observed in the progeny of bystander cells that had been in co-culture with cells irradiated with iron ions, but not with protons.

  8. Morphometric and functional abnormalities of kidneys in the progeny of mice fed chocolate during pregnancy and lactation.

    Directory of Open Access Journals (Sweden)

    Ewa Skopińska-Rózewska

    2006-09-01

    Full Text Available Even most commonly consumed beverages like tea, coffee, chocolate and cocoa contain methylxanthines, biogenic amines and polyphenols, among them catechins, that exhibit significant biological activity and might profoundly affect the organism homeostasis. We have previously shown that 400 mg of bitter chocolate or 6 mg of theobromine added to the daily diet of pregnant and afterwards lactating mice affected embryonic angiogenesis and caused bone mineralization disturbances as well as limb shortening in 4-weeks old offspring. The aim of the present study was the morphometric and functional evaluation of kidneys in the 4-weeks old progeny mice fed according to the protocol mentioned above. Progeny from the mice fed chocolate presented considerable morphometric abnormalities in the kidney structure, with the lower number of glomeruli per mm2 and their increased diameter. Moreover, higher serum creatinine concentration was observed in that group of offspring. No morphometric or functional irregularities were found in the progeny of mice fed theobromine. Abnormalities demonstrated in the offspring of mice fed chocolate are not related to its theobromine content. Consequently, identification of active compound(s responsible for the observed effects is of vital importance.

  9. Electrophoretic karyotypes of Tilletia caries, T. controversa, and their F1 progeny: further evidence for conspecific status.

    Science.gov (United States)

    Russell, B W; Mills, D

    1993-01-01

    Electrophoretic karyotypes were obtained from intact sporidia and mycelia of Tilletia controversa and T. caries, and hybrid progeny were obtained by crossing these pathogens. The chromosomes typically ranged from approximately 850 to 4,490 kilobases (kb) for all strains, and they were variable in number with 19 or 20 for strains of T. controversa, 14-20 for T. caries, and from 19 to 22 for the hybrid progeny. The estimated genome size varied from 28 to 42 megabases (Mb) for these strains. Radiolabeled probes made of single copy DNA fragments and a heterologous actin gene identified four linkage groups among all strains that exhibited maximum chromosome length polymorphisms of 14% or less. The chromosomes carrying the rDNA genes, representing a fifth linkage group, exhibited length polymorphisms of approximately 40%. The actin gene and a rDNA probe hybridized with one or more bands in these strains, suggesting that some of the variability in chromosome number may result from aneuploidy. The karyotypes of the hybrid progeny revealed chromosome numbers and genome sizes essentially identical to each parental strain, clearly indicating that the reduction division stage of meiosis had occurred. These data and other corroborative genetic data provide substantial evidence that T. controversa and T. caries are not different species, but variants of a single species.

  10. Anaphylaxis: risk factors for recurrence.

    Science.gov (United States)

    Mullins, R J

    2003-08-01

    There are few studies on the incidence or recurrence of anaphylaxis. To examine the incidence of anaphylaxis and risk factors for recurrence. A prospective study of 432 patients referred to a community-based specialist practice in the Australian Capital Territory with anaphylaxis, followed by a survey to obtain information on recurrence. Of 432 patients (48% male, 73% atopic, mean 27.4 years, SD 19.5, median 26) with anaphylaxis, 260 patients were seen after their first episode; 172 experienced 584 previous reactions. fifty-four percent of index episodes were treated in hospital. Aetiology was identified in 91.6% patients: food (61%), stinging insects (20.4%) or medication (8.3%). The minimum occurrence and incidence of new cases of anaphylaxis was estimated at 12.6 and 9.9 episodes/100,000 patient-years, respectively. Follow-up data were obtained from 304 patients (674 patient-years). One hundred and thirty experienced further symptoms (45 serious), 35 required hospitalization and 19 administered adrenaline. Accidental ingestion of peanut/tree nut caused the largest number of relapses, but the highest risk of recurrence was associated with sensitivity to wheat and/or exercise. Rates of overall and serious recurrence were 57 and 10 episodes/100 patient-years, respectively. Of those prescribed adrenaline, 3/4 carried it, 2/3 were in date, and only 1/2 patients faced with serious symptoms administered adrenaline. Five patients each developed new triggers for anaphylaxis, or re-presented with significant psychiatric symptoms. In any 1 year, 1/12 patients who have suffered anaphylaxis will experience recurrence, and 1/50 will require hospital treatment or use adrenaline. Compliance with carrying and using adrenaline is poor. Occasional patients develop new triggers or suffer psychiatric morbidity.

  11. Genome-assisted prediction of a quantitative trait measured in parents and progeny: application to food conversion rate in chickens

    Directory of Open Access Journals (Sweden)

    Rosa Guilherme JM

    2009-01-01

    Full Text Available Abstract Accuracy of prediction of yet-to-be observed phenotypes for food conversion rate (FCR in broilers was studied in a genome-assisted selection context. Data consisted of FCR measured on the progeny of 394 sires with SNP information. A Bayesian regression model (Bayes A and a semi-parametric approach (Reproducing kernel Hilbert Spaces regression, RKHS using all available SNPs (p = 3481 were compared with a standard linear model in which future performance was predicted using pedigree indexes in the absence of genomic data. The RKHS regression was also tested on several sets of pre-selected SNPs (p = 400 using alternative measures of the information gain provided by the SNPs. All analyses were performed using 333 genotyped sires as training set, and predictions were made on 61 birds as testing set, which were sons of sires in the training set. Accuracy of prediction was measured as the Spearman correlation (r¯S MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGaciGaaiaabeqaaeqabiWaaaGcbaGafmOCaiNbaebadaWgaaWcbaGaem4uamfabeaaaaa@2EB5@ between observed and predicted phenotype, with its confidence interval assessed through a bootstrap approach. A large improvement of genome-assisted prediction (up to an almost 4-fold increase in accuracy was found relative to pedigree index. Bayes A and RKHS regression were equally accurate (r¯S MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGaciGaaiaabeqaaeqabiWaaaGcbaGafmOCaiNbaebadaWgaaWcbaGaem4uamfabeaaaaa@2EB5@ = 0.27 when all 3481 SNPs were included in the model. However, RKHS with 400 pre-selected informative SNPs was more accurate than Bayes A with all SNPs.

  12. Galactosemia presenting as recurrent sepsis.

    Science.gov (United States)

    Rathi, Narendra; Rathi, Akanksha

    2011-12-01

    Galactosemia is a treatable metabolic disorder caused by the deficiency of enzyme galactose-1-phosphate uridyl transferase (GALT) and inherited as an autosomal recessive trait. A case of neonate manifesting with recurrent Escherichia coli sepsis is presented here which turned out to be a classic galactosemia. No other common presenting features were observed in this infant except cataract on slit lamp examination. To the best of our knowledge, there is no case of galactosemia reported in literature which presented with recurrent neonatal sepsis without hepatomegaly, hyperbilirubinemia, bleeding disorder, vomiting, diarrhea, failure to thrive, hypoglycemia, coagulopathy, hemolysis or renal tubular acidosis.

  13. The influence of alpha-zearalenol on magnesium concentration in cellular subfractions of certain tissues of female rats and their progeny.

    Science.gov (United States)

    Pasternak, Kazimierz; Kiełczykowska, Małgorzata; Sztanke, Małgorzata; Borzecki, Andrzej

    2003-06-01

    The purpose of the research work was to study the influence of alpha-zearalenol on magnesium concentration in cellular subfractions of certain tissues of female rats and their progeny. Pregnant female rats received alpha-zearalenol at the doses of: 0.05, 0.5 or 1 mg x kg(-1) b.w. After delivery, brain, liver and kidneys of female rats and their progeny were removed and magnesium concentration was measured in cellular subfractions of these tissues. Alpha-zearalenol reduced Mg-level in all the cellular subfractions of female rat tissues. In progeny tissues, alpha-zearalenol decreased magnesium concentration in nuclear subfraction only. In mitochondrial and cytosol subfractions it increased Mg-concentration. The changes in magnesium concentration were depended on alpha-zearalenol doses and tissue nature. The magnesium concentration decrease was higher in female rats than in progeny tissues.

  14. Beneficial effect of feeding a ketogenic diet to mothers on brain development in their progeny with a murine model of pyruvate dehydrogenase complex deficiency

    Directory of Open Access Journals (Sweden)

    Lioudmila Pliss

    2016-06-01

    Conclusion: The findings provide for the first time experimental support for beneficial effects of a ketogenic diet during the prenatal and early postnatal periods on the brain development of PDC-deficient mammalian progeny.

  15. Particle size distribution of the radon progeny and ambient aerosols in the Underground Tourist Route “Liczyrzepa” Mine in Kowary Adit

    Directory of Open Access Journals (Sweden)

    Wołoszczuk Katarzyna

    2018-01-01

    Full Text Available Central Laboratory for Radiological Protection, in cooperation with Central Mining Institute performed measurements of radon concentration in air, potential alpha energy concentration (PAEC, particle size distribution of the radon progeny and ambient aerosols in the Underground Tourist-Educational Route “Liczyrzepa” Mine in Kowary Adit. A research study was developed to investigate the appropriate dose conversion factors for short-lived radon progeny. The particle size distribution of radon progeny was determined using Radon Progeny Particle Size Spectrometer (RPPSS. The device allows to receive the distribution of PAEC in the particle size range from 0.6 nm to 2494 nm, based on their activity measured on 8 stages composed of impaction plates or diffusion screens. The measurements of the ambient airborne particle size distribution were performed in the range from a few nanometres to about 20 micrometres using Aerodynamic Particle Sizer (APS spectrometer and the Scanning Mobility Particle Sizer Spectrometer (SMPS.

  16. A method for determining an indicator of effective dose calculation due to inhalation of Radon and its progeny from in vivo measurements

    CERN Document Server

    Estrada, J

    1994-01-01

    Direct measurement of the absolved dose to lung tissue from inhalation of radon and its progeny is not possible and must be calculated using dosimetric models, taking into consideration the several parameters upon which the dose calculation depends. To asses the dose due to inhalation of radon and its progeny, it is necessary to estimate the cumulative exposure. Historically, this has been done using WLM values estimated with measurements of radon concentration in air. The radon concentration in air varies significantly, however, in space with time, and the exposed individual is also constantly moving around. This makes it almost impossible to obtain a precise estimate of an individual's inhalation exposure. This work describes a pilot study to calculate lung dose from the deposition of radon progeny, via estimates of cumulative exposure derived from in vivo measurements of sup 2 sup 1 sup 0 Pb, in subjects exposed to above-average radon and its progeny concentrations in their home environments. The measureme...

  17. Red deer cloned from antler stem cells and their differentiated progeny.

    Science.gov (United States)

    Berg, Debra K; Li, Chunyi; Asher, Geoff; Wells, David N; Oback, Björn

    2007-09-01

    The significance of donor cell differentiation status for successful cloning by somatic cell nuclear transfer (SCNT) is unclear. Here, we cloned a new species, red deer (Cervus elaphus), from multipotent antler stem cells and their differentiated progeny. Cultured donor cell lines from male antlerogenic periosteum (AP) were left undifferentiated or chemically induced to initiate osteogenesis or adipogenesis. Based on their morphology and marker gene expression profile, donor cells were classified as undifferentiated AP cells, presumptive osteoblasts, or adipocytes. Adipocytes upregulated adipogenic markers procollagen type I alpha 2 (COL1A2), peroxisome proliferator-activated receptor gamma 2 (PPARG), and gylceraldehyde-3-phosphate dehydrogenase (GAPDH), and downregulated antlerogenic transcripts POU-domain class 5 transcription factor (POU5F1) and parathyroid hormone (PTH)-like hormone (PTHLH). Despite differences prior to NT, transcript abundance of donor-specific markers COL1A2, PPARG, GAPDH, and POU5F1 did not differ significantly in cloned blastocysts (P = 0.10, 0.50, 0.61, and 0.16, respectively). However, donor cell and blastocyst expression levels were completely different for most genes analyzed, indicating their successful reprogramming. The type of donor cell used for NT (AP, bone, and fat cells), had no effect on in vitro development to blastocysts (93 [38%] of 248 vs. 32 [44%] of 73 vs. 59 [32%] of 183, respectively). Likewise, development to weaning was not significantly different between the three cell types (2 [4%] of 46 vs. 2 [29%] of 7 vs. 4 [13%] of 31, for AP vs. bone vs. fat, respectively). Microsatellite DNA analysis confirmed that the eight cloned red deer calves were genetically identical to the cells used for NT.

  18. Study of the atmospheric chemistry of radon progeny in laboratory and real indoor atmospheres

    Energy Technology Data Exchange (ETDEWEB)

    Hopke, P.K.

    1992-07-01

    This report describes studies on the chemical and physical behavior of the [sup 218]Po atom immediately following its formation by the alpha decay of radon. Because small changes in size for activity in the sub-10 nm size range result in large changes in the delivered dose per unit exposure, this behavior must be understood if the exposure to radon progeny and its dose to the cells in the respiratory tract are to be fully assessed. The specific tasks of the controlled laboratory studies are to determine the formation rates of [center dot]OH radicals formed by the radiolysis of air following radon decay, to examine the formation of particles by the radiolytic oxidation of substances like SO[sub 2] ethylene, and H[sub 2]S to lower vapor pressure compounds and determine the role of gas phase additives such as H[sub 2]O and NH[sub 3] in determining the particle size, to measure the rate of ion-induced nucleation using a thermal diffusion cloud chamber, and to measure the neutralization rate of [sup 218]Po[sub x][sup +] in O[sub 2] at low radon concentrations. Tasks of the exposure studies in occupied indoor spaces are to initiate measurements of the activity size distributions in actual homes with occupants present so that the variability of the indoor activity size distributions can be assessed with respect to indoor aerosol sources and general lifestyle variations of the occupants, to initiate a prospective study of the utility of measurement of deposited [sup 210]Pb embedded in glass surfaces as a measure of the long-term, integrated exposure of the population to radon, and to develop the methodology to determine the hygroscopicity of the indoor aerosol so that the changes in deposition efficiency of the radioactive indoor aerosol with hygroscopic growth in the respiratory tract can be assessed.

  19. Perioperative morbidity and outcome of secondary cytoreduction for recurrent epithelial ovarian cancer

    NARCIS (Netherlands)

    Woelber, L.; Jung, S.; Eulenburg, C.; Mueller, V.; Schwarz, J.; Jaenicke, F.; Mahner, S.

    Background: Despite radical surgical and chemotherapeutic treatment of ovarian cancer, the majority of patients develop recurrent disease. Secondary cytoreductive surgery can result in favourable outcome in selected patients, but information regarding feasibility, safety and perioperative outcome of

  20. Prediction of Bladder Cancer Recurrences Using Artificial Neural Networks

    Science.gov (United States)

    Zulueta Guerrero, Ekaitz; Garay, Naiara Telleria; Lopez-Guede, Jose Manuel; Vilches, Borja Ayerdi; Iragorri, Eider Egilegor; Castaños, David Lecumberri; de La Hoz Rastrollo, Ana Belén; Peña, Carlos Pertusa

    Even if considerable advances have been made in the field of early diagnosis, there is no simple, cheap and non-invasive method that can be applied to the clinical monitorisation of bladder cancer patients. Moreover, bladder cancer recurrences or the reappearance of the tumour after its surgical resection cannot be predicted in the current clinical setting. In this study, Artificial Neural Networks (ANN) were used to assess how different combinations of classical clinical parameters (stage-grade and age) and two urinary markers (growth factor and pro-inflammatory mediator) could predict post surgical recurrences in bladder cancer patients. Different ANN methods, input parameter combinations and recurrence related output variables were used and the resulting positive and negative prediction rates compared. MultiLayer Perceptron (MLP) was selected as the most predictive model and urinary markers showed the highest sensitivity, predicting correctly 50% of the patients that would recur in a 2 year follow-up period.

  1. Recurrence quantification analysis of chimera states

    Energy Technology Data Exchange (ETDEWEB)

    Santos, M.S. [Pós-Graduação em Ciências/Física, Universidade Estadual de Ponta Grossa, 84030-900, Ponta Grossa, PR (Brazil); Szezech, J.D., E-mail: jdanilo@gmail.com [Departamento de Matemática e Estatística, Universidade Estadual de Ponta Grossa, 84030-900, Ponta Grossa, PR (Brazil); Batista, A.M., E-mail: antoniomarcosbatista@gmail.com [Departamento de Matemática e Estatística, Universidade Estadual de Ponta Grossa, 84030-900, Ponta Grossa, PR (Brazil); Caldas, I.L. [Instituto de Física, Universidade de São Paulo, 05315-970, São Paulo, SP (Brazil); Viana, R.L.; Lopes, S.R. [Departamento de Física, Universidade Federal do Paraná, 81531-990, Curitiba, PR (Brazil)

    2015-10-02

    Chimera states, characterised by coexistence of coherence and incoherence in coupled dynamical systems, have been found in various physical systems, such as mechanical oscillator networks and Josephson-junction arrays. We used recurrence plots to provide graphical representations of recurrent patterns and identify chimera states. Moreover, we show that recurrence plots can be used as a diagnostic of chimera states and also to identify the chimera collapse. - Highlights: • Chimera states have been found in various physical systems. • Recurrence plots is a graphical method useful to locate recurring patterns. • We used recurrence plots to identify the chimera states. • We show also the recurrence plots can identify the chimera collapse.

  2. Statistical modelling for recurrent events: an application to sports injuries

    Science.gov (United States)

    Ullah, Shahid; Gabbett, Tim J; Finch, Caroline F

    2014-01-01

    Background Injuries are often recurrent, with subsequent injuries influenced by previous occurrences and hence correlation between events needs to be taken into account when analysing such data. Objective This paper compares five different survival models (Cox proportional hazards (CoxPH) model and the following generalisations to recurrent event data: Andersen-Gill (A-G), frailty, Wei-Lin-Weissfeld total time (WLW-TT) marginal, Prentice-Williams-Peterson gap time (PWP-GT) conditional models) for the analysis of recurrent injury data. Methods Empirical evaluation and comparison of different models were performed using model selection criteria and goodness-of-fit statistics. Simulation studies assessed the size and power of each model fit. Results The modelling approach is demonstrated through direct application to Australian National Rugby League recurrent injury data collected over the 2008 playing season. Of the 35 players analysed, 14 (40%) players had more than 1 injury and 47 contact injuries were sustained over 29 matches. The CoxPH model provided the poorest fit to the recurrent sports injury data. The fit was improved with the A-G and frailty models, compared to WLW-TT and PWP-GT models. Conclusions Despite little difference in model fit between the A-G and frailty models, in the interest of fewer statistical assumptions it is recommended that, where relevant, future studies involving modelling of recurrent sports injury data use the frailty model in preference to the CoxPH model or its other generalisations. The paper provides a rationale for future statistical modelling approaches for recurrent sports injury. PMID:22872683

  3. Statistical modelling for recurrent events: an application to sports injuries.

    Science.gov (United States)

    Ullah, Shahid; Gabbett, Tim J; Finch, Caroline F

    2014-09-01

    Injuries are often recurrent, with subsequent injuries influenced by previous occurrences and hence correlation between events needs to be taken into account when analysing such data. This paper compares five different survival models (Cox proportional hazards (CoxPH) model and the following generalisations to recurrent event data: Andersen-Gill (A-G), frailty, Wei-Lin-Weissfeld total time (WLW-TT) marginal, Prentice-Williams-Peterson gap time (PWP-GT) conditional models) for the analysis of recurrent injury data. Empirical evaluation and comparison of different models were performed using model selection criteria and goodness-of-fit statistics. Simulation studies assessed the size and power of each model fit. The modelling approach is demonstrated through direct application to Australian National Rugby League recurrent injury data collected over the 2008 playing season. Of the 35 players analysed, 14 (40%) players had more than 1 injury and 47 contact injuries were sustained over 29 matches. The CoxPH model provided the poorest fit to the recurrent sports injury data. The fit was improved with the A-G and frailty models, compared to WLW-TT and PWP-GT models. Despite little difference in model fit between the A-G and frailty models, in the interest of fewer statistical assumptions it is recommended that, where relevant, future studies involving modelling of recurrent sports injury data use the frailty model in preference to the CoxPH model or its other generalisations. The paper provides a rationale for future statistical modelling approaches for recurrent sports injury. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  4. Interpretation of Recurrent Neural Networks

    DEFF Research Database (Denmark)

    Pedersen, Morten With; Larsen, Jan

    1997-01-01

    This paper addresses techniques for interpretation and characterization of trained recurrent nets for time series problems. In particular, we focus on assessment of effective memory and suggest an operational definition of memory. Further we discuss the evaluation of learning curves. Various nume...

  5. MRI of recurrent rectosigmoid carcinoma.

    Science.gov (United States)

    Markus, J; Morrissey, B; deGara, C; Tarulli, G

    1997-01-01

    A prospective study was performed to determine the most reliable MRI criteria to distinguish recurrent rectosigmoid carcinoma from benign postoperative fibrosis. Twenty-two consecutive patients who were suspected to have recurrent rectosigmoid carcinoma were examined by T2 and precontrast and contrast enhanced T1 weighted images. The prospective interpretations, the presence of high signal on T2 weighted images, the shape of the margins of a mass and the degree of contrast enhancement were correlated with histology and follow up to determine their respective accuracies, sensitivities and specificities. The best criteria for recurrent tumor was the combination of high signal on T2 weighted images, round margins and > 40% contrast enhancement, which had an accuracy of 92%, sensitivity of 100% and specificity of 85%. In patients who were more than one year postoperative the specificity was 100%. The most reliable MRI criteria for distinguishing recurrent rectosigmoid carcinoma from benign postoperative fibrosis are the combination of the signal intensity on T2 weighed images, the shape of the margins of a mass and the presence of greater than 40% contrast enhancement.

  6. Recurrent processing during object recognition

    Directory of Open Access Journals (Sweden)

    Randall C. O'Reilly

    2013-04-01

    Full Text Available How does the brain learn to recognize objects visually, and perform this difficult feat robustly in the face of many sources of ambiguity and variability? We present a computational model based on the biology of the relevant visual pathways that learns to reliably recognize 100 different object categories in the face of of naturally-occurring variability in location, rotation, size, and lighting. The model exhibits robustness to highly ambiguous, partially occluded inputs. Both the unified, biologically plausible learning mechanism and the robustness to occlusion derive from the role that recurrent connectivity and recurrent processing mechanisms play in the model. Furthermore, this interaction of recurrent connectivity and learning predicts that high-level visual representations should be shaped by error signals from nearby, associated brain areas over the course of visual learning. Consistent with this prediction, we show how semantic knowledge about object categories changes the nature of their learned visual representations, as well as how this representational shift supports the mapping between perceptual and conceptual knowledge. Altogether, these findings support the potential importance of ongoing recurrent processing throughout the brain's visual system and suggest ways in which object recognition can be understood in terms of interactions within and between processes over time.

  7. Recurrent pericarditis: current challenges and future prospects

    Directory of Open Access Journals (Sweden)

    Dauphin C

    2016-07-01

    Full Text Available Claire Dauphin,1 Etienne Merlin,2 Aurélie Chalard,1 Romain Trésorier,1 Jean-René Lusson,1 Martin Soubrier3 1Department of Cardiology and Cardiovascular Diseases, 2Department of Pediatrics, 3Department of Rheumatology, Clermont-Ferrand Hospital, Clermont-Ferrand, France Abstract: Recurrent pericarditis, usually idiopathic, is difficult to treat. Previous etiological studies can eliminate potentially serious causes that require specific treatment. The pathophysiology of “idiopathic” pericarditis involves autoimmunity and autoinflammation in various degrees and is usually caused by a microbacteriological or postinjury trigger. It requires multidisciplinary care to provide individualized treatment to each patient: treatment escalation by increments, then weaning should be guided by clinical examination but also CRP and potential magnetic resonance imaging. The role of immunomodulators, especially anakinra, should be revisited. Their efficacy is remarkable, and they could be considered for second-line treatment, preferably with corticosteroids (at least among certain selected patients, especially after the failure of prolonged and high-dose standard therapy with aspirin/nonsteroidal anti-inflammatory drugs and colchicine. Keywords: recurrent pericarditis, review, pathophysiology, individualized treatment, multidisciplinary care, treatment options 

  8. An analysis of phenotypic selection in natural stands of northern red oak (Quercus rubra L.)

    Science.gov (United States)

    Jeffery W. Stringer; David B. Wagner; Scott E. Schlarbaum; Daniel B. Houston

    1995-01-01

    Comparison of growth and stem quality parameters of 19-year-old progeny from superior and comparison trees indicates that rigorous phenotypic selection of trees in natural stands may not be an efficient method of parent tree selection for Quercus rubra L. Total tree height, dbh, number of branches in the butt log, fork height, and number of mainstem...

  9. Stereotactic Radiosurgery in the Treatment of Recurrent CNS Lymphoma.

    Science.gov (United States)

    Kumar, Ravi; Laack, Nadia; Pollock, Bruce E; Link, Michael; O'Neill, Brian P; Parney, Ian F

    2015-08-01

    Central nervous system lymphoma (CNSL) is typically treated with chemotherapy and external beam radiation therapy (EBRT). Optimal management of recurrent CNSL is poorly defined. We aim to determine the effectiveness of stereotactic radiosurgery in the treatment of recurrent CNSL. We performed a retrospective analysis of patients having stereotactic radiosurgery (SRS) for recurrent intracranial CNSL between 1999 and 2011. Fourteen patients (20 tumors) underwent SRS. The median patient age was 71 years (range, 18-82 years). Seven patients (50%) had primary CNSL. All had received prior chemotherapy, and nine patients (64%) had undergone prior whole brain radiotherapy (WBRT) with a median dose of 40 Gy in 25 fractions. The median preoperative Karnofsky Performance Score was 80. The median tumor volume was 6.7 cm(3) (range, 0.5-37.7); the median margin dose was 15.5 Gy (range, 10-18). Eleven patients (79%) had tumor regression (n = 11) shown on MRI after SRS. The median overall survival after SRS was 9.5 months. No patient had a major radiation-related complication. Stereotactic radiosurgery for patients with recurrent CNSL is well tolerated and has a high rate of radiographic response. A marginal dose of 15.5 Gy or greater, a Karnofsky score of 80 or greater, and the absence of pre-SRS WBRT were associated with significantly longer overall survival. Stereotactic radiosurgery may be beneficial as an initial salvage therapy in the treatment of recurrent CNSL for properly selected patients. Copyright © 2015 Elsevier Inc. All rights reserved.

  10. How adaptation shapes spike rate oscillations in recurrent neuronal networks

    Directory of Open Access Journals (Sweden)

    Moritz eAugustin

    2013-02-01

    Full Text Available Neural mass signals from in-vivo recordings often show oscillations with frequencies ranging from <1 Hz to 100 Hz. Fast rhythmic activity in the beta and gamma range can be generated by network based mechanisms such as recurrent synaptic excitation-inhibition loops. Slower oscillations might instead depend on neuronal adaptation currents whose timescales range from tens of milliseconds to seconds. Here we investigate how the dynamics of such adaptation currents contribute to spike rate oscillations and resonance properties in recurrent networks of excitatory and inhibitory neurons. Based on a network of sparsely coupled spiking model neurons with two types of adaptation current and conductance based synapses with heterogeneous strengths and delays we use a mean-field approach to analyze oscillatory network activity. For constant external input, we find that spike-triggered adaptation currents provide a mechanism to generate slow oscillations over a wide range of adaptation timescales as long as recurrent synaptic excitation is sufficiently strong. Faster rhythms occur when recurrent inhibition is slower than excitation and oscillation frequency increases with the strength of inhibition. Adaptation facilitates such network based oscillations for fast synaptic inhibition and leads to decreased frequencies. For oscillatory external input, adaptation currents amplify a narrow band of frequencies and cause phase advances for low frequencies in addition to phase delays at higher frequencies. Our results therefore identify the different key roles of neuronal adaptation dynamics for rhythmogenesis and selective signal propagation in recurrent networks.

  11. TS expression predicts postoperative recurrence in adenocarcinoma of the lung.

    Science.gov (United States)

    Shimokawa, Hidehiko; Uramoto, Hidetaka; Onitsuka, Takamitsu; Iwata, Teruo; Nakagawa, Makoto; Ono, Kenji; Hanagiri, Takeshi

    2011-06-01

    Not all patients with lung cancer require postoperative adjuvant chemotherapy after a complete resection. However, no useful markers for either selecting appropriate candidates or for predicting clinical recurrence exist. Tumor specimens were collected from 183 consecutive patients who underwent a complete resection for lung adenocarcinoma from 2003 to 2007 in our department. We analyzed the thymidylate synthase (TS) and dihydrofolate reductase (DHFR) expressions in the primary lung adenocarcinoma by immunohistochemisty. The strong expression of TS and DHFR was identified in 39 (21.3%) and 120 (65.6%) patients, respectively. The strong TS expression was identified in 11 (39.3%) of 28 patients and 28 (18.1%) of 155 patients in patients with and without recurrence, respectively (p=0.012). The strong DHFR expression was also identified in 23 (82.1%) and 97 (62.6%) of the patients with and without recurrence, respectively (p=0.045). Logistic regression models indicated the strong TS expression to be an independent factor for tumor recurrence. The strong TS and DHFR expression was associated with a poorer disease-free survival (DFS) according to the survival analysis. A multivariate analysis demonstrated the strong TS expression to be independently associated with an increased risk for poor DFS. The strong TS expression may be a useful marker for predicting postoperative recurrence in patients with lung adenocarcinoma following surgery. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

  12. Recurrent uncomplicated urinary tract infections in women: a review.

    Science.gov (United States)

    Nosseir, Sandy B; Lind, Lawrence R; Winkler, Harvey A

    2012-03-01

    Recurrent urinary tract infections most often present with symptoms of irritative voiding. In most cases, they are caused by reinfection with a previously isolated organism. Patients with one or more symptoms of uncomplicated recurrent urinary tract infection should undergo thorough examination and screening for underlying comorbidities that increase susceptibility. When frequent reinfections, empiric treatment relapse, persistent infections, or risk factors for complicated infections are encountered, patients may benefit from urodynamics, cystoscopy, renal ultrasound, intravenous urogram, or voiding cystourethrogram to evaluate for anatomic, functional, or metabolic abnormalities affecting the urinary tract (e.g., stones, stricture, obstruction, vesicoureteral reflux, lesions, detrusor underactivity). These patients may benefit from culture-guided empiric treatment and further evaluation by urology, nephrology, or infectious disease specialists. In patients with a history of uncomplicated urinary tract infections, empiric treatment guided by local antimicrobial resistance may efficiently treat a suspected recurrence. After successful treatment of the acute infection, postcoital prophylaxis, continuous prophylaxis, or self-start empiric treatment may be selected based on frequency of recurrent infections, temporal relation to intercourse, and patient characteristics. Ancillary measures such as probiotics, cranberry products, or local estrogen replacement may also be considered. This article will review the current definition, epidemiology, pathogenesis, diagnosis, work-up, treatment, treatment side effects, and prevention of recurrent urinary tract infections in women. A suggested algorithm for evaluation and treatment based on current literature is provided.

  13. Whole-body diffusion-weighted magnetic resonance imaging (WB-DW-MRI) vs choline-positron emission tomography-computed tomography (choline-PET/CT) for selecting treatments in recurrent prostate cancer.

    Science.gov (United States)

    Conde-Moreno, A J; Herrando-Parreño, G; Muelas-Soria, R; Ferrer-Rebolleda, J; Broseta-Torres, R; Cozar-Santiago, M P; García-Piñón, F; Ferrer-Albiach, C

    2017-05-01

    To determine the effectiveness of whole-body diffusion-weighted magnetic resonance imaging (WB-DW-MRI) in detecting metastases by comparing the results with those from choline-positron emission tomography-computed tomography (choline-PET/CT) in patients with biochemical relapse after primary treatment, and no metastases in bone scintigraphy, CT and/or pelvic MRI, or metastatic/oligometastatic prostate cancer (PCa). Patients with this disease profile who could benefit from treatment with stereotactic body radiation therapy (SBRT) were selected and their responses to these techniques were rated. This was a prospective, controlled, unicentric study, involving 46 consecutive patients from our centre who presented biochemical relapse after adjuvant, salvage or radical treatment with external beam radiotherapy, or brachytherapy. After initial tests (bone scintigraphy, CT, pelvic MRI), 35 patients with oligometastases or without them were selected. 11 patients with multiple metastases were excluded from the study. WB-DW-MRI and choline-PET/CT was then performed on each patient within 1 week. The results were interpreted by specialists in nuclear medicine and MRI. If they were candidates for treatment with ablative SBRT (SABR), they were then evaluated every three months with both tests. Choline-PET/CT detected lesions in 16 patients that were not observable using WB-DW-MRI. The results were consistent in seven patients and in three cases, a lesion was observed using WB-DW-MRI that was not detected with choline-PET/CT. The Kappa value obtained was 0.133 (p = 0.089); the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of WB-DW-MRI were estimated at 44.93, 64.29, 86.11, and 19.15%, respectively. For choline-PET/CT patients, the sensitivity, specificity, PPV, and NPV were 97.10, 58.33, 93.06, and 77.78%, respectively. Choline-PET/CT has a high global sensitivity while WB-DW-MRI has a high specificity, and so they are

  14. Giant recurrent retroperitoneal liposarcoma presenting as a recurrent inguinal hernia

    Directory of Open Access Journals (Sweden)

    Ajay H. Bhandarwar

    2011-11-01

    Full Text Available Retroperitoneal liposarcoma presenting as an inguinal hernia is a rare entity. We present the first case of Giant recurrent liposarcoma presenting as a recurrent inguinal hernia in a 40-year-old male. Physical examination showed an irreducible lump in the right inguinal region and a scar in the right lumbar and right inguinal region. Computed tomography (CT scan of abdomen revealed it to be a retro peritoneal mass extending into the right inguinal region along and involving the cord structures. Wide local excision of the tumour with right orchidectomy and inguinal hernioplasty was performed. Histo-pathology confirmed it to be a liposarcoma. Patient received postoperative radio therapy. Follow up of two years has shown him to be disease free. Retroperitoneal liposarcoma can grow along cord structures into the inguinal canal and mimic an irreducible indirect inguinal hernia.

  15. Fifth International Symposium on Recurrence Plot

    CERN Document Server

    Riley, Michael; Giuliani, Alessandro; Webber, Charles; Jr, Jr; Translational Recurrences : From Mathematical Theory to Real-World Applications

    2014-01-01

    This book features 13 papers presented at the Fifth International Symposium on Recurrence Plots, held August 2013 in Chicago, IL. It examines recent applications and developments in recurrence plots and recurrence quantifi cation analysis (RQA) with special emphasis on biological and cognitive systems and the analysis of coupled systems using cross-recurrence methods. Readers will discover new applications and insights into a range of systems provided by recurrence plot analysis and new theoretical and mathematical developments in recurrence plots. Recurrence plot based analysis is a powerful tool that operates on real-world complex systems that are nonlinear, non-stationary, noisy, of any statistical distribution, free of any particular model type, and not particularly long. Quantitative analyses promote the detection of system state changes, synchronized dynamical regimes, or classifi cation of system states. Th e book will be of interest to an interdisciplinary audience of recurrence plot users and researc...

  16. Temperature, age, and recurrence of febrile seizure

    NARCIS (Netherlands)

    M. van Stuijvenberg (Margriet); E.W. Steyerberg (Ewout); G. Derksen-Lubsen (Gerarda); H.A. Moll (Henriëtte)

    1998-01-01

    textabstractOBJECTIVE: Prediction of a recurrent febrile seizure during subsequent episodes of fever. DESIGN: Study of the data of the temperatures, seizure recurrences, and baseline patient characteristics that were collected at a randomized placebo controlled trial of ibuprofen

  17. Genetic parameter and correlation estimates of processing traits in half-sib progenies of tropical-adapted carrot germplasm Parâmetros genéticos e correlações entre características para processamento em progênies de meios-irmãos de germoplasma de cenoura tropical

    Directory of Open Access Journals (Sweden)

    Jairo V Vieira

    2012-03-01

    Full Text Available The estimate of the genetic parameters associated with processing (fresh-cut traits, including root length (RL, is crucial for carrot breeding programs in tropical areas. The cultivar Alvorada is an important germplasm due to its resistance to nematodes, leaf blight, heat-tolerance, and high carotenoid content. Seventy-four 'Alvorada' half-sib progenies were evaluated during the summer of 2005 in the Federal District, Brazil, in a randomized complete block design with three replications. Thirteen competitive plants in each block were randomly selected and evaluated and/or classified for RL and for number of leaves (NL, leaf length (LL, root tip type (RT, root mass (RW, crown shape (CS, root diameter (RD, and xylem diameter (XD. The Pearson's correlation coefficients and the heritability values were estimated for all traits. The path analysis was also used considering the RL trait as dependent variable. The heritability for RL ranged from 12 to 44%. For the other traits, the values ranged from 3% (RD to 79% (LL. Phenotypic and genotypic correlations among all traits were low to intermediate. Path analysis indicated positive direct relationship between RL and RW, whereas RD and XD displayed negative direct effect on RL. Longer roots had narrow diameter and narrow XD. Recurrent selection based upon either half-sib or S1 families would be more effective than mass phenotypic recurrent selection in increasing RL and to develop populations expressing multiple desirable processing traits in tropical-adapted carrot germplasm.A estimativa de parâmetros genéticos associados com caracteres de processamento industrial, incluindo comprimento de raiz (RL, é crucial para programas de melhoramento de cenoura para áreas tropicais. A cultivar Alvorada é um importante germoplasma devido à sua resistência a nematóides e queima-das-folhas, tolerância ao calor e alto conteúdo de carotenóides. Setenta e quatro progênies meio-irmãs derivadas de 'Alvorada

  18. Is balance impaired by recurrent sprained ankle?

    Science.gov (United States)

    Isakov, E; Mizrahi, J

    1997-01-01

    OBJECTIVE: To evaluate uninjured and recurrent sprained ankles during single leg standing, both with and without visual input, and the contribution of related proprioceptive feedback in this event. METHODS: A force measuring system was used for monitoring reaction forces in the anteroposterior and mediolateral directions during single leg standing. Differences between selected variables obtained in the uninjured and sprained ankles were analysed using two way analysis of variance. RESULTS: Foot-ground reaction forces in both anteroposterior and mediolateral directions were the same in normal and sprained ankles of each subject while standing with either open or closed eyes. However, standing with closed eyes, irrespective of the ankle status, always produced significantly higher reaction forces than those obtained with open eyes (P leg standing is similar in the chronically sprained and the uninjured ankle joint. Images p66-a PMID:9132216

  19. Relationship between recurrent dreams and ego identity

    OpenAIRE

    森田, 修平; 松下, 姫歌

    2011-01-01

    The purpose of this study was to examine the relationship between recurrent dreams and ego identity. It is said that recurrent dreams can tell dreamers about their problems. In adolescence, people may face problems such as Erikson's developmental task for achieving ego identity. If having a recurrent dream implies working toward achieving a developmental task, it would imply that young people who have recurrent dreams NOW are working toward achieving a PREVIOUS developmental task. Two hundred...

  20. On the interaction between radon progeny and particles generated by electronic and traditional cigarettes

    Science.gov (United States)

    Vargas Trassierra, C.; Cardellini, F.; Buonanno, G.; De Felice, P.

    2015-04-01

    During their entire lives, people are exposed to the pollutants present in indoor air. Recently, Electronic Nicotine Delivery Systems, mainly known as electronic cigarettes, have been widely commercialized: they deliver particles into the lungs of the users but a "second-hand smoke" has yet to be associated to this indoor source. On the other hand, the naturally-occurring radioactive gas, i.e. radon, represents a significant risk for lung cancer, and the cumulative action of these two agents could be worse than the agents separately would. In order to deepen the interaction between radon progeny and second-hand aerosol from different types of cigarettes, a designed experimental study was carried out by generating aerosol from e-cigarette vaping as well as from second-hand traditional smoke inside a walk-in radon chamber at the National Institute of Ionizing Radiation Metrology (INMRI) of Italy. In this chamber, the radon present in air comes naturally from the floor and ambient conditions are controlled. To characterize the sidestream smoke emitted by cigarettes, condensation particle counters and scanning mobility particle sizer were used. Radon concentration in the air was measured through an Alphaguard ionization chamber, whereas the measurement of radon decay product in the air was performed with the Tracelab BWLM Plus-2S Radon daughter Monitor. It was found an increase of the Potential Alpha-Energy Concentration (PAEC) due to the radon decay products attached to aerosol for higher particle number concentrations. This varied from 7.47 ± 0.34 MeV L-1 to 12.6 ± 0.26 MeV L-1 (69%) for the e-cigarette. In the case of traditional cigarette and at the same radon concentration, the increase was from 14.1 ± 0.43 MeV L-1 to 18.6 ± 0.19 MeV L-1 (31%). The equilibrium factor increases, varying from 23.4% ± 1.11% to 29.5% ± 0.26% and from 30.9% ± 1.0% to 38.1 ± 0.88 for the e-cigarette and traditional cigarette, respectively. These growths still continue for long

  1. Genetic analysis of growth traits in the progeny of rabbit does from a diallel cross

    Directory of Open Access Journals (Sweden)

    C. Mínguez

    2015-12-01

    Full Text Available An experiment was carried out to estimate the genetic group effects and the crossbreeding genetic parameters of growth traits [body weight (BW, average daily gain (ADG, feed intake (FI and feed conversion ratio (FCR] in rabbits during fattening between 28 and 63 d. The rabbits were the progeny of does coming from a full diallel cross between 4 maternal lines (A, V, H and LP mated to bucks of the paternal line R. On thousand fifty five  rabbits were controlled and the traits were recorded weekly, with the cage as the experimental unit for FI and FCR (283 cages. The genetic group (V line was present in all farms in order to connect records among them and to be used as reference group. Crossbreeding parameters were estimated according to the Dickerson model. Regarding dam effects between pure lines for BW at weaning, A line was the heaviest and showed significant differences with LP and V lines (61 and 30 g, respectively. Upon completion of the fattening period, the differences in favour of A line for BW at weaning were compensated. Throughout the fattening period, no significant differences were observed between the lines as dam lines. At the end of the fattening period, no significant differences were observed between the crossbred groups. Regarding the reciprocal effects, the most relevant results were the significances for FCR in favour of H as sire line. For all traits, the confidence intervals at 95% of all contrast and effects were large. The estimates of maternal heterosis were, in general, negative, which could be a consequence of the positive heterosis for litter size. The AH cross showed significant maternal heterosis for BW at 43 d (–53 g, ADG between 28 and 42 d (–3.5 g/d, FI between 28 and 63 d (–7 g/d and FCR between 42 and 63 d (–0.15. The combination of direct and maternal effects of the V line was the worst for all growth traits, showing significant differences with the LP line for most of them, for instance 0.13 worse

  2. Increased SLIT immunoreactivity as a biomarker for recurrence in endometrial carcinoma.

    Science.gov (United States)

    Ma, Shulan; Liu, Xishi; Geng, Jian-Guo; Guo, Sun-Wei

    2010-01-01

    We sought to investigate the potential predictive value of SLIT/ROBO1 immunoreactivity in recurrent and nonrecurrent endometrial cancer (EC), and the relationship between SLIT/Roundabout (ROBO1) immunoreactivity and microvessel density (MVD) in EC. From a total of 815 consecutive patients histologically diagnosed with EC who had undergone surgery we retrieved 45 patients who had confirmed recurrence and randomly selected 110 patients without recurrence. Their paraffin-embedded tissue blocks were also retrieved and subjected to immunohistochemistry for pan-SLIT and ROBO1. MVD counts were evaluated by CD34 immunohistochemistry. Univariate and multivariate analyses were performed to evaluate the effect of SLIT/ROBO1 on recurrence risk with adjustment for other known risk factors. Immunoreactivity to pan-SLIT and ROBO1 was higher in recurrence patients than that in nonrecurrence patients. Both SLIT and ROBO1 immunoreactivities were positively correlated with MVD. Cox regression analysis identified SLIT, along with age and International Federation of Gynecology and Obstetrics stage, as risk factors for recurrence. The resultant discrimination model yielded estimated and cross-validated sensitivity and specificity of 79% and 85%, respectively. Increased immunoreactivity to SLIT is an important factor for recurrence of EC, likely through attracting endothelial cells and promoting neovascularization. Thus, the SLIT immunoreactivity is likely a promising biomarker for recurrence and the SLIT/ROBO1 system may be a potential target for reducing the recurrence risk in EC. 2010 Mosby, Inc.

  3. Genetic Evolution of Glioblastoma Stem-Like Cells From Primary to Recurrent Tumor.

    Science.gov (United States)

    Orzan, Francesca; De Bacco, Francesca; Crisafulli, Giovanni; Pellegatta, Serena; Mussolin, Benedetta; Siravegna, Giulia; D'Ambrosio, Antonio; Comoglio, Paolo M; Finocchiaro, Gaetano; Boccaccio, Carla

    2017-11-01

    Glioblastoma (GBM) is a lethal tumor that displays remarkable genetic heterogeneity. It is also known that GBM contains a cell hierarchy driven by GBM stem-like cells (GSCs), responsible for tumor generation, therapeutic resistance, and relapse. An important and still open issue is whether phylogenetically related GSCs can be found in matched primary and recurrent GBMs, and reflect tumor genetic evolution under therapeutic pressure. To address this, we analyzed the mutational profile of GSCs isolated from either human primary GBMs (primary GSCs) or their matched tumors recurring after surgery and chemoradiotherapy (recurrent GSCs). We found that recurrent GSCs can accumulate temozolomide-related mutations over primary GSCs, following both linear and branched patterns. In the latter case, primary and recurrent GSCs share a common set of lesions, but also harbor distinctive mutations indicating that primary and recurrent GSCs derive from a putative common ancestor GSC by divergent genetic evolution. Interestingly, TP53 mutations distinctive of recurrent GSCs were detectable at low frequency in the corresponding primary tumors and likely marked pre-existent subclones that evolved under therapeutic pressure and expanded in the relapsing tumor. Consistently, recurrent GSCs displayed in vitro greater therapeutic resistance than primary GSCs. Overall, these data indicate that (a) phylogenetically related GSCs are found in matched primary and recurrent GBMs and (b) recurrent GSCs likely pre-exist in the untreated primary tumor and are both mutagenized and positively selected by chemoradiotherapy. Stem Cells 2017;35:2218-2228. © 2017 AlphaMed Press.

  4. VESPRO: An Individual Patient Data Prospective Meta-Analysis of Selective Internal Radiation Therapy Versus Sorafenib for Advanced, Locally Advanced, or Recurrent Hepatocellular Carcinoma of the SARAH and SIRveNIB Trials.

    Science.gov (United States)

    Gebski, Val; Gibbs, Emma; Gandhi, Mihir; Chatellier, Gilles; Dinut, Aurelia; Pereira, Helena; Chow, Pierce Kh; Vilgrain, Valérie

    2017-02-15

    Untreated advanced hepatocellular carcinoma (HCC) has an overall poor prognosis. Currently there are 2 ongoing prospective randomized controlled trials that are evaluating the efficacy and safety of sorafenib and selective internal radiation therapy (SIRT) with yttrium-90 resin microspheres in patients with advanced HCC. The SorAfenib versus Radioembolisation in Advanced Hepatocellular carcinoma (SARAH; 459 patients) trial is being performed in Europe and the SIRt VErsus SorafeNIB (SIRveNIB; 360 patients) trial in the Asia Pacific region. Prospectively combining the results, these trials will not only allow for increased precision to estimate efficacy (in terms of survival), but will also provide increased statistical power for subgroup analyses. To ensure the prospectivity and transparency of the meta-analysis. The sirVEnib and SARAH merge PROject (VESPRO) is an individual, patient-data prospective meta-analysis of the SIRveNIB and SARAH randomized trials. The VESPRO protocol includes prespecified hypotheses, inclusion criteria, and outcome measures. The primary outcome measure is overall survival and secondary outcomes include tumor response rate, progression-free survival, progression in the liver as first event, and disease control in the liver. Pooling of toxicity results will allow for robust safety profiles to be established for both therapies, and provides increased statistical power to investigate treatment effects in key subgroups. Analyses will be performed in the intent-to-treat population stratified by trial. Both studies are expected to demonstrate a survival benefit for SIRT together with a better toxicity profile compared with sorafenib. It is also anticipated that liver progression as the first event would be longer in the intervention compared with the control. As the results of the 2 trials are not yet known, the methodological strength is enhanced, as biases inherent in conventional meta-analyses are avoided. This has the effect of providing

  5. Early diffuse recurrence of hepatocellular carcinoma after percutaneous radiofrequency ablation: analysis of risk factors

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hee Young; Rhim, Hyunchul; Lee, Min Woo; Kim, Young-sun; Choi, Dongil; Park, Min Jung; Kim, Young Kon; Kim, Seong Hyun; Lim, Hyo Keun [Samsung Medical Center, Sungkyunwan University, School of Medicine, Department of Radiology and Center for Imaging Science, Seoul (Korea, Republic of)

    2013-01-15

    To evaluate the risk factors affecting early diffuse recurrence within 1 year of percutaneous ultrasound-guided radiofrequency ablation (RFA) for hepatocellular carcinoma (HCC). Out of 146 patients who received transcatheter arterial chemoembolisation (TACE) for treatment of recurrent HCC after percutaneous ultrasound-guided RFA, we selected 23 patients with early diffuse recurrence. Early diffuse recurrence was defined as three or more new recurrent HCCs within 1 year of initial RFA. As a control group, we selected 23 patients, matched exactly for age and sex, in which there was no local tumour progression or new recurrence after RFA. To analyse the risk factors, we examined patient factors and tumour factors. Recurrent tumours occurred from 30 to 365 days after RFA (median time, 203 days). Univariate analysis indicated that larger tumour size and poorly defined margin were significant risk factors (P < 0.05). Multivariate analysis indicated that poorly defined margin was a significant risk factor (P < 0.05). Larger tumour size and poorly defined margin may be risk factors for early diffuse recurrence of HCC within 1 year of RFA. Tumours with such risk factors should be treated with a combination of TACE to minimise the potential for therapeutic failure. (orig.)

  6. Recurrent cervical cancer : detection and prognosis

    NARCIS (Netherlands)

    Duyn, A; Van Eijkeren, M; Kenter, G; Zwinderman, K; Ansink, A

    Background. Only a small proportion of cervical cancer recurrences is detected during routine follow-up. We investigated which percentage of recurrences is detected during follow-up, which diagnostic tools are helpful to detect recurrent disease and which factors are of prognostic significance once

  7. Stitch Abscess Masquerading as Recurrent Thyroid Cancer ...

    African Journals Online (AJOL)

    Recurrent thyroid cancer after remnant ablation is a known entity in follow up of differentiated thyroid cancer. It is however unusual for a stitch abscess to present as a recurrent thyroid cancer. We highlight the diagnostic dilemma of a stitch abscess masquerading as a recurrent thyroid cancer in a young female adult in the ...

  8. Recurrent impetigo herpetiformis: case report.

    Science.gov (United States)

    Wamalwa, Emmanuel Wekesa

    2017-01-01

    Impetigo herpetiformis (pustular psoriasis of pregnancy) is a rare dermatosis of pregnancy that typically starts in the 2nd half of pregnancy and resolves postpartum. It may recur in subsequent pregnancies. I present a case of 23 year old female gravida 4 para 3 with recurrent impetigo herpetiformis at 26 weeks gestation. She presented with a one month history of pustular lesions which responded to treatment with prednisone. She delivered at term with a favourable outcome. The disease resolved one month postpartum. This was the second recurrence of the disease. She had her first episode of impetigo herpetiformis during the second pregnancy. The disease recurred in the 3rd pregnancy and resulted in a still birth.

  9. Recurrent Dislocation of the Patella

    Science.gov (United States)

    Benítez, Gustavo

    2015-01-01

    Purpose: To evaluate results of medial patellofemoral ligament (MPFL) reconstruction associated with lateral release and advancement of vastus medialis in recurrent dislocation of the patella. Methods: We retrospectively evaluated 11 patients with a mean follow-up of 19 months. Mean age was 23, mainly women. We did MPFL reconstruction with semitendinosus or gracilis tendon depending on BMI, associated with advancement of vastus medialis and lateral release. Results: Mean Kujala score improved from 46,54 pts. preoperative to 88,36 postoperative. Our main complication was 1 patient with rigid knee, who required movilization under anesthesia and arthroscopic arthrolisis to improve her outcome. Conclusion: The combination of this techniques are a good alternative to treat patients with recurrent patella disclocation, with good short and mid-term results. Biomechanic intra and postop complications of MPFL reconstruction are related to patellar fixation, anatomic positioning of femoral tunnel and knee position of the graft fixation.

  10. Clopidogrel-Induced Recurrent Polyarthritis

    Directory of Open Access Journals (Sweden)

    Sahil Agrawal MD

    2013-08-01

    Full Text Available Clopidogrel is an oral thienopyridine and together with aspirin is a component of dual antiplatelet therapy for the prevention of stent thrombosis after intracoronary stent placement. The common adverse effects from its use are an increased risk of bleeding, neutropenia, and rash. Arthralgia and backache are also known to occur with its use. There have been case reports linking arthritis with the use of clopidogrel. We describe the case of a 64-year-old man who reported symptoms of fever and joint pains following initiation of therapy with clopidogrel. Acute-phase reactants were elevated. Laboratory and radiologic testing were unremarkable. Incidentally, he reported experiencing a similar arthritis after he received a loading dose of clopidogrel prior to a diagnostic coronary angiography in the past. The symptoms improved dramatically on discontinuation of clopidogrel. There was no recurrence of symptoms with prasugrel. This describes possibly the second incidence of recurrent arthritis with clopidogrel therapy.

  11. Herpetic Recurrent Oral Erythema Multiforme

    OpenAIRE

    Shantala Arunkumar; Savitha S.Shettar; Mamata G.P; Rajeshwari G. Annigeri; Shakuntala G. K

    2016-01-01

    Erythema multiforme is considered as an immunologic disease possibly occurring because of predisposition to certain microorganisms, radiotherapy, systemic diseases, malignancy, and food or drug allergy. Here we report a case of herpes-induced recurrent erythema multiforme primarily manifesting in oral mucosa during first two episodes and third episode was characterized by oral lesions followed by skin lesions and the severity was increased with the subsequent episod...

  12. Herpetic Recurrent Oral Erythema Multiforme

    Directory of Open Access Journals (Sweden)

    Shantala Arunkumar

    2016-04-01

    Full Text Available Erythema multiforme is considered as an immunologic disease possibly occurring because of predisposition to certain microorganisms, radiotherapy, systemic diseases, malignancy, and food or drug allergy. Here we report a case of herpes-induced recurrent erythema multiforme primarily manifesting in oral mucosa during first two episodes and third episode was characterized by oral lesions followed by skin lesions and the severity was increased with the subsequent episodes, during each incident we have successfully managed the case.

  13. Recurrent Dislocation of the Patella

    OpenAIRE

    Ben?tez, Gustavo

    2015-01-01

    Purpose: To evaluate results of medial patellofemoral ligament (MPFL) reconstruction associated with lateral release and advancement of vastus medialis in recurrent dislocation of the patella. Methods: We retrospectively evaluated 11 patients with a mean follow-up of 19 months. Mean age was 23, mainly women. We did MPFL reconstruction with semitendinosus or gracilis tendon depending on BMI, associated with advancement of vastus medialis and lateral release. Results: Mean Kujala score improved...

  14. Recurrent angioedema - a case report

    OpenAIRE

    Martins, Sandrina; Salgado, Miguel; Raposo, Filipa; Pinto, Diana; Martinho, Isabel; Araújo, Ana Rita

    2016-01-01

    Introduction: Hereditary angioedema (HA) is a rare cause of recurrent angioedema caused by a default in the gene that encodes the C1 esterase inhibitor (C1-INH). The oedema involves predominantly the face, limbs and genital and gastrointestinal tract. The involvement of the larynx, although less frequent, is the most severe clinical expression of HA and is potentially fatal. Case report: Clinical report of an eight-year-old female with multiple episodes of angioedema. The...

  15. Recurrent Respiratory Infections in Children

    Directory of Open Access Journals (Sweden)

    F. Yurochko

    2012-02-01

    Full Text Available The paper covers a problem of recurrent respiratory infections (RRI in children. Their description, risk factors, diagnostic algorithm have been dwelt. A special attention is paid to the treatment. An optimal antibiotic in RRI of bacterial genesis is a high-dose amoxicillin/clavulanate (registered as Augmentin™ ES in Ukraine, the efficacy of which is 94.6–96.3 % according to different data.

  16. Measurment of radon, thoron and their progeny in indoor environment of Mohali, Punjab, Northern India, using pinhole dosimeters

    Directory of Open Access Journals (Sweden)

    Mehta Vimal

    2016-01-01

    Full Text Available The health hazards of radon and its decay products above certain levels are well known. However, for any preventive measures to be taken, we have to be aware of radon levels of that particular area. Measurement of radon and its decay products in indoor environments is an important aspect of assessing indoor air quality and health conditions associated with it. Keeping this in mind, measurements of radon, thoron and their progeny concentrations were carried out in Mohali, Northern India, using pinhole-based twin cup dosimeters. Radon exhalation rates of soil samples in the dwellings/areas were measured via an active technique of a continuous radon monitor. The indoor radon concentration in Mohali varied from 15.03 ± 0.61 Bq/m3 to 39.21 ± 1.46 Bq/m3 with an average of 26.95 Bq/m3 ,while thoron concentration in the same dwellings varied from 9.62 ± 0.54 Bq/m3 to 52.84 ± 2.77 Bq/m3 with an average of 31.09 Bq/m3. Radon progeny levels in dwellings under study varied from 1.63 to 4.24 mWL, with an average of 2.94 mWL, while thoron progeny levels varied from 0.26 to 1.43 mWL , with an average of 0.84 mWL. The annual dose received by the inhabitants of dwellings under study varied from 0.78 to 2.36 mSv, with an average of 1.61 mSv. The in situ gamma dose rate varied from 0.12 to 0.32 mSv/h.

  17. Recurrence Risk of Hyperemesis Gravidarum

    Science.gov (United States)

    2011-01-01

    The purpose of this study is to describe the recurrence risk for hyperemesis gravidarum (HG). Women who registered on a website sponsored by the Hyperemesis Education and Research Foundation as having had one HG-complicated pregnancy were contacted to follow-up on a subsequent pregnancy. One hundred women responded. Fifty-seven had become pregnant again, 2 were trying to conceive, 37 were not willing to get pregnant again due to HG, and 4 did not have a 2nd pregnancy for other reasons. Among the 57 women who responded that they had become pregnant again, 81% reported having severe nausea and vomiting in their second pregnancy. Among the women reporting recurrent HG, 98% reported losing weight and taking prescribed medication for HG, 83% reported treatment with IV fluids, 20% reported treatment with TPN or NG tube feeding, and 48% reported hospitalization for HG. This study demonstrates both a high recurrence rate and a large percentage of women who change reproductive plans due to their experience with hyperemesis gravidarum. PMID:21429077

  18. His-tag based in situ labelling of progeny viruses for real-time single virus tracking in living cells.

    Science.gov (United States)

    Zheng, Lin Ling; Li, Chun Mei; Zhen, Shu Jun; Li, Yuan Fang; Huang, Cheng Zhi

    2016-11-10

    Tracking virus infection events in live cells is useful for understanding the mechanism of virus infection, and fluorescent labelling is a critical step. Herein a noninvasive strategy for labelling viruses with His-tags was developed by in situ modifying the cell surface proteins with polypeptides containing His-tags during progeny virus assembly. The His-tagged viruses were further conjugated with Ni2+-nitrilotriacetate complex modified quantum dots, and retained their infectivity for real-time single virus tracking in living cells.

  19. Effect of Organic Selenium from Se-enriched Alga (Chlorella spp. on Selenium Transfer from Sows to Their Progeny

    Directory of Open Access Journals (Sweden)

    Martin Svoboda

    2009-01-01

    Full Text Available The study was conducted to determine the efficacy of organic Se from Se-enriched alga Chlorella spp. in placental transfer to piglets. In group A (n = 8 the sows were fed during the gestation a diet supplemented with inorganic Se (sodium selenite, 0.3 mg/kg. In group B (n = 8 the diet of the sows was supplemented with organic Se from Se-enriched alga (0.3 mg/kg. The Se concentrations in the whole blood (P P Chlorella spp. in sows resulted in greater transfer of Se to their progeny.

  20. Genomic instability induced in distant progeny of bystander cells depends on the connexins expressed in the irradiated cells.

    Science.gov (United States)

    de Toledo, Sonia M; Buonanno, Manuela; Harris, Andrew L; Azzam, Edouard I

    2017-10-01

    To examine the time window during which intercellular signaling though gap junctions mediates non-targeted (bystander) effects induced by moderate doses of ionizing radiation; and to investigate the impact of gap junction communication on genomic instability in distant progeny of bystander cells. A layered cell culture system was developed to investigate the propagation of harmful effects from irradiated normal or tumor cells that express specific connexins to contiguous bystander normal human fibroblasts. Irradiated cells were exposed to moderate mean absorbed doses from 3.7 MeV α particle, 1000 MeV/u iron ions, 600 MeV/u silicon ions, or 137Cs γ rays. Following 5 h of co-culture, pure populations of bystander cells, unexposed to secondary radiation, were isolated and DNA damage and oxidative stress was assessed in them and in their distant progeny (20-25 population doublings). Increased frequency of micronucleus formation and enhanced oxidative changes were observed in bystander cells co-cultured with confluent cells exposed to either sparsely ionizing (137Cs γ rays) or densely ionizing (α particles, energetic iron or silicon ions) radiations. The irradiated cells propagated signals leading to biological changes in bystander cells within 1 h of irradiation, and the effect required cellular coupling by gap junctions. Notably, the distant progeny of isolated bystander cells also exhibited increased levels of spontaneous micronuclei. This effect was dependent on the type of junctional channels that coupled the irradiated donor cells with the bystander cells. Previous work showed that gap junctions composed of connexin26 (Cx26) or connexin43 (Cx43) mediate toxic bystander effects within 5 h of co-culture, whereas gap junctions composed of connexin32 (Cx32) mediate protective effects. In contrast, the long-term progeny of bystander cells expressing Cx26 or Cx43 did not display elevated DNA damage, whereas those coupled by Cx32 had enhanced DNA damage

  1. Levels of radon gas concentration and progeny in homes of Potosi City, Bolivia to 4000 m; Niveles de concentracion de gas radon y progenie en viviendas de la Ciudad de Potosi, Bolivia a 4000 msnm

    Energy Technology Data Exchange (ETDEWEB)

    Mamani M, R. [Universidad Autonoma Tomas Frias, Carrera de Fisica, Av. del maestro s/n, Edif. Central Potosi, Villa Imperial de Potosi (Bolivia, Plurinational State of); Claros J, J. [Universidad Autonoma Tomas Frias, Facultad de Minas Potosi, Centro de Investigacion, Av. Serrudo y Arce s/n, Villa Imperial de Potosi (Bolivia, Plurinational State of); Vasquez A, R., E-mail: raulm2k13@hotmail.com [Instituto Boliviano de Biologia de Altura, Calle Hoyos 953, La Paz (Bolivia, Plurinational State of)

    2015-10-15

    Full text: In this work the presence of radon gas was determined, which is a radioactive contaminant that comes from underground, able to penetrate the houses. The danger is that when mixed air and when inhaled can cause serious damage to the lungs, for the short life time that has radon and progeny for decay, damaging the pulmonary alveoli and reducing breathing capacity of the habitants, then causing polycythemia in some cases. The study was carried out in homes in the city of Potosi, Bolivia located at 4000 m. The quantification of radon gas and progeny was performed with the equipment Alpha-Zaeller-2 (Az-2), quantification was realized in 6 zones of the city of Potosi, chosen randomly. In each zone were carried out measurements in 40 homes (2 rooms more permanent), both day and night, for a period of 3 days in two different seasons and with concentrations of average humidity of 20, 50 and 80%. The values obtained for each period vary depending on the season and 30 to 50% of the allowable values given by the EPA and Who for housing. (Author)

  2. Survey on Factors Affecting the Recurrence of Multiple sclerosis Attacks

    Directory of Open Access Journals (Sweden)

    Hasanzadeh F

    2015-02-01

    Full Text Available Background and Objective: Multiple sclerosis (MS is a chronic and progressive disease of the central nervous system. The identification of effective factors and patient education on the prevention of exposure to these factors can be effective in reducing relapses. Therefore, the present study aimed to investigate the factors that affect recurrence in patients with multiple sclerosis. Materials and Method: This cross-sectional study was conducted on 402 MS patients referred to the MS Association of Mashhad, Iran, in 2013. The study participants were selected using purposive sampling method. Data collection tools included a demographic questionnaire and a 40-item researcher-made questionnaire concerning factors affecting the recurrence of MS. The 40-item questionnaire consisted of 4 domains personal factors, medical condition, pharmaceutical conditions, and environmental factors. Data analysis was performed using SPSS software version 21 and Mann Whitney, Pearson correlation, Kruskal Wallis, and chi-square statistical tests. Results: Among the patients, 21/4% were male and 78/6% female. Most patients were 20-29 years old. The highest prevalence of recurrence of MS was observed in the summer (70.8% and in August (50.3%. There was no significant relationship between patient’s gender and their views about the relationship between infections, fatigue, and heat and recurrence of MS. However, a higher rate of women believed stress (P < 0.029 and sexual development (P < 0.001 to be effective on the incidence of MS recurrence, in comparison to men. Conclusion: Most patients with MS are women, and they believe stress to be more effective on the incidence of MS attack recurrence. Therefore, nurses, as the primary care educators, can improve the patients’ capability, disease management, and quality of life through training of stress management techniques.

  3. Stress Reduction in Improving Quality of Life in Patients With Recurrent Gynecologic or Breast Cancer

    Science.gov (United States)

    2015-10-08

    Anxiety Disorder; Depression; Fatigue; Leydig Cell Tumor; Ovarian Sarcoma; Ovarian Stromal Cancer; Pain; Peritoneal Carcinomatosis; Pseudomyxoma Peritonei; Recurrent Breast Cancer; Recurrent Cervical Cancer; Recurrent Endometrial Carcinoma; Recurrent Fallopian Tube Cancer; Recurrent Gestational Trophoblastic Tumor; Recurrent Ovarian Epithelial Cancer; Recurrent Ovarian Germ Cell Tumor; Recurrent Primary Peritoneal Cavity Cancer; Recurrent Uterine Sarcoma; Recurrent Vaginal Cancer; Recurrent Vulvar Cancer

  4. Combined family selection for tolerance to corn reddening

    Directory of Open Access Journals (Sweden)

    Bekavac Goran

    2010-01-01

    Full Text Available Variability and interrelationships of traits in maize population NS 1-275 CRS after one cycle of combined HS-S1 family selection were studied. Independent field experiments with HS and S1 progenies were set up according to an incomplete block design in 2008 and 2009. The basic genetics parameters of zero (C0 and the first cycle of selection (C1 were assessed. The primary selection criterion for the formation of the C1 was grain yield (GY and grain moisture (GM and secondary stay green (SG and stalk lodging (SL. Significant differences in mean values between selection cycles (P£0.05 were determined for SG at S1 progenies and GY and SG at HS progenies. No significant changes in genetic variances were recorded after one cycle of selection. Positive correlations between GY and SG and negative between SG and SL exclude the occurrence of negative correlative response. In the upcoming period, some other agronomic traits have to be analyzed to get complete picture of their complex interrelationships, as well as the expected changes through cycles of selection. .

  5. Contribution of problem-solving skills to fear of recurrence in breast cancer survivors.

    Science.gov (United States)

    Akechi, Tatuo; Momino, Kanae; Yamashita, Toshinari; Fujita, Takashi; Hayashi, Hironori; Tsunoda, Nobuyuki; Iwata, Hiroji

    2014-05-01

    Although fear of recurrence is a major concern among breast cancer survivors after surgery, no standard strategies exist that alleviate their distress. This study examined the association of patients' problem-solving skills and fear of recurrence and psychological distress among breast cancer survivors. Randomly selected, ambulatory, female patients with breast cancer participated in this study. They were asked to complete the Concerns about Recurrence Scale (CARS) and the Hospital Anxiety and Depression Scale. Multiple regression analyses were used to examine their associations. Data were obtained from 317 patients. Patients' problem-solving skills were significantly associated with all subscales of fear of recurrence and overall worries measured by the CARS. In addition, patients' problem-solving skills were significantly associated with both their anxiety and depression. Our findings warrant clinical trials to investigate effectiveness of psychosocial intervention program, including enhancing patients' problem-solving skills and reducing fear of recurrence among breast cancer survivors.

  6. A prognostic index for locoregional recurrence after neoadjuvant chemotherapy

    OpenAIRE

    Herrero-Vicent, C; Guerrero-Zotano, A; Gavil?-Gregori, J; Hern?ndez-Blanquisett, A; Sandiego-Contreras, S; Samper-Hiraldo, JM; Guillem-Porta, V; Ruiz-Sim?n, A

    2016-01-01

    Background The appropriate selection criteria for breast-conserving surgery (BCS) or mastectomy after neoadjuvant chemotherapy (NAC) are poorly defined. The aim of this study is to analyse the incidence and prognostic factors for locoregional recurrence (LRR) in patients with breast cancer (BC) treated with NAC to develop a prognostic score to help with clinical decision-making. Materials and methods Using our retrospective maintained BC database, we identified 730 patients treated with NAC (...

  7. A correlation of long term effects and radiation quality in the progeny of bystander cells after microbeam radiations: The experimental study of radiotherapy for cancer risk mitigation

    Science.gov (United States)

    Autsavapromporn, N.; Konishi, T.; Liu, C.; Plante, I.; Funayama, T.; Usami, N.; Azzam, EI; Suzuki, M.

    2017-06-01

    The goal of this study is to investigate the role of radiation quality and gap junction intercellular communication (GJIC) in the propagation of delayed stressful effects in the progeny of bystander human skin fibroblasts cultures (NB1RGB). Briefly, confluent NB1RGB cells in the presence and absence of gap junction inhibitor (AGA) were exposed to ionizing radiation (IR) with a different linear energy transfer (LET) either 5.35 keV X rays (LET ∼6 keV/μm) or 18.3 MeV/u carbon (LET ∼103 keV/μm) microbeam radiations. Following 20 populations post-irradiation, the progeny of bystander NB1RGB cells were harvested and assayed for several of biological endpoints. Our results showed that expression of stressful effects in the progeny of bystander cells is dependent on LET. The progeny of bystander cells exposed to low-LET X rays showed the persistence of oxidative stress and it was correlated with the increased mutant fraction. Such effect were not observed after high-LET carbon ions. Interestingly, inhibition of GJIC mitigated the toxic effects in the progeny of bystander cells. Together, the results contribute to the understanding of the fundamental radiation biology relating to the high-LET carbon ions to mitigate cancer risk after radiotherapy. Furthermore, GJIC be considered as a critical mediator in the bystander mutagenic effect.

  8. Avaliação de progênies de algodoeiro para resistência à broca-da-raiz Progeny evaluation for resistance to the root borer in cotton

    Directory of Open Access Journals (Sweden)

    Imre Lajos Gridi-Papp

    1996-01-01

    Full Text Available Com base nos resultados de seleção, obtidos pela equipe de melhoramento do algodoeiro do Instituto Agronômico (IAC, numa gleba naturalmente infestada pela broca-da-raiz, Eutinobothrus brasiliensis (Hambleton, da Estação Experimental de Tietê, em 1966-81, três índices de avaliação foram propostos para a resistência dessa planta à praga. O coeficiente de correlação simples entre as duas repetições dos testes anuais de progênies foi adotado como parâmetro para estudar a eficiência dos índices na seleção de progênies resistentes. Pelo estudo comparativo, escolheu-se, como o mais adequado, o índice obtido pela contagem de furos (galerias observados na parte lenhosa, em corte oblíquo dos caules das plantas, feito na região do colo, no meio da parte hipertrofiada. Apresentam-se os coeficientes de correlação de 1972-81 e a discussão sobre a eficiência do índice escolhido que proporcionou, durante o período, um aumento gradual do coeficiente de correlação, significando um provável acúmulo de genes favoráveis à resistência nas progênies selecionadas. A prática de picar e enterrar a parte infestada dos caules, após a colheita dos dados, contribuiu para manter alta infestação da área pela broca. Aplicado, posteriormente, à avaliação de linhagens do programa de melhoramento do algodoeiro, o índice permitiu melhorar 50% o nível geral de resistência das linhagens em estudo.To evaluate cotton resistance to the Brazilian root borer, Eutinobothrus brasiliensis (Hambleton, three indexes were proposed, based on selection data obtained in progeny tests, on a naturally infested plot, situated at the Tietê Experiment Station, in the years of 1966 to 1981, by the cotton breeders of the Instituto Agronômico, State of São Paulo, Brazil. The simple correlation coefficient between the two repetitions of the yearly tests was used as a parameter to study the efficiency of the indexes in selecting resistant progenies

  9. Relationships among performance, residual feed intake, and product quality of progeny from Red Angus sires divergent for maintenance energy EPD.

    Science.gov (United States)

    Welch, C M; Ahola, J K; Hall, J B; Murdoch, G K; Crews, D H; Davis, L C; Doumit, M E; Price, W J; Keenan, L D; Hill, R A

    2012-12-01

    Energy expenditure is a physiological process that may be closely associated with residual feed intake (RFI). The maintenance energy (ME(M)) EPD was developed by the Red Angus Association of America (RAAA) and is used as an indicator of energy expenditure. The objectives of this study were to evaluate and quantify the following relationships using progeny of Red Angus (RA) sires divergent for ME(M) EPD: 1) postweaning RFI and finishing phase feed efficiency (FE), 2) postweaning RFI and end-product quality, and 3) postweaning RFI and sire ME(M) EPD. A total of 12 RA sires divergent for ME(M) EPD were chosen using the RAAA-generated ME(M) EPD values and were partitioned into 2 groups: high ME(M) EPD (≥4 Mcal/mo) and low ME(M) EPD ( 0.05) with any carcass traits or end-product quality measurements. Sire ME(M) EPD was phenotypically correlated (P 0.05) between progeny postweaning RFI and sire ME(M) EPD. Therefore, results suggest 1) RFI measured during the postweaning growth phase is indicative of FE status in the finishing phase, 2) neither RFI nor sire ME(M) EPD negatively affected carcass or end-product quality, and 3) RFI and sire ME(M) EPD are not phenotypically associated.

  10. Search for major genes with progeny test data to accelerate the development of genetically superior loblolly pine

    Energy Technology Data Exchange (ETDEWEB)

    NCSU

    2003-12-30

    This research project is to develop a novel approach that fully utilized the current breeding materials and genetic test information available from the NCSU-Industry Cooperative Tree Improvement Program to identify major genes that are segregating for growth and disease resistance in loblolly pine. If major genes can be identified in the existing breeding population, they can be utilized directly in the conventional loblolly pine breeding program. With the putative genotypes of parents identified, tree breeders can make effective decisions on management of breeding populations and operational deployment of genetically superior trees. Forest productivity will be significantly enhanced if genetically superior genotypes with major genes for economically important traits could be deployed in an operational plantation program. The overall objective of the project is to develop genetic model and analytical methods for major gene detection with progeny test data and accelerate the development of genetically superior loblolly pine. Specifically, there are three main tasks: (1) Develop genetic models for major gene detection and implement statistical methods and develop computer software for screening progeny test data; (2) Confirm major gene segregation with molecular markers; and (3) Develop strategies for using major genes for tree breeding.

  11. Measurement of short-lived radon progenies by simultaneous alpha gamma-spectrometry at the German radon reference chamber

    CERN Document Server

    Paul, A; Honig, A; Sulima, T; Buchholz, A; Keyser, U

    1999-01-01

    In the German radon reference chamber, the short-lived radon progenies are separated by a sample tube according to the attached or unattached fraction, while their activity concentration is afterwards measured by simultaneous alpha- and gamma-spectrometry. The results are expressed by the equilibrium factor F and the unattached fraction f sub p (International Commission on Radiological Protection, ICRP Publication 50, Ann. ICRP 17 (1987) 1). Both F and f sub p , can be therefore studied with respect to the full set of environmental parameters, e.g. temperature, humidity, air pressure and aerosol concentration (Honig et al., Nucl. Instr. and Meth. A 416 (1998) 525). Up to now, well-defined and stable equilibrium factors in the interval from 0.1 to 1.0 have been established. In correlation with this, the unattached fraction can be varied from 0.01 to 0.9. The sample and measuring technique for the short-lived radon progenies described in this work is the basis for fundamental studies with regard to the equilibr...

  12. Sperm DNA fragmentation, recurrent implantation failure and recurrent miscarriage

    Directory of Open Access Journals (Sweden)

    Carol Coughlan

    2015-01-01

    Full Text Available Evidence is increasing that the integrity of sperm DNA may also be related to implantation failure and recurrent miscarriage (RM. To investigate this, the sperm DNA fragmentation in partners of 35 women with recurrent implantation failure (RIF following in vitro fertilization, 16 women diagnosed with RM and seven recent fathers (control were examined. Sperm were examined pre- and post-density centrifugation by the sperm chromatin dispersion (SCD test and the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL assay. There were no significant differences in the age of either partner or sperm concentration, motility or morphology between three groups. Moreover, there were no obvious differences in sperm DNA fragmentation measured by either test. However, whilst on average sperm DNA fragmentation in all groups was statistically lower in prepared sperm when measured by the SCD test, this was not seen with the results from the TUNEL assay. These results do not support the hypothesis that sperm DNA fragmentation is an important cause of RIF or RM, or that sperm DNA integrity testing has value in such patients. It also highlights significant differences between test methodologies and sperm preparation methods in interpreting the data from sperm DNA fragmentation tests.

  13. Delayed-type hypersensitivity response to KLH in F2 and backcrosses of two immune selected chicken lines: effect of immunisation and selection

    NARCIS (Netherlands)

    Minozzi, G.; Parmentier, H.K.; Bed'hom, B.; Minvielle, F.; Gourichon, D.; Pinard-van der Laan, M.H.

    2008-01-01

    Cell-mediated immune response to keyhole limpet hemocyanin (KLH) was studied in 13 different progeny groups belonging to a second generation cross between two lines of White Leghorn previously selected for 10 generations for high antibody response to Newcastle disease vaccine (ND3) and high

  14. Recurrence of Hyperprolactinemia after Withdrawal of Long-Term Cabergoline Therapy

    Science.gov (United States)

    Kharlip, J.; Salvatori, R.; Yenokyan, G.; Wand, G. S.

    2009-01-01

    Context: Recurrence of hyperprolactinemia after cabergoline withdrawal ranges widely from 36 to 80%. The Pituitary Society recommends withdrawal of cabergoline in selected patients. Objective: Our aim was to evaluate recurrence of hyperprolactinemia in patients meeting The Pituitary Society guidelines. Design: Patients were followed from the date of discontinuation to either relapse of hyperprolactinemia or the day of last prolactin test. Setting: We conducted the study at an academic medical center. Patients: Forty-six patients meeting Pituitary Society criteria (normoprolactinemic and with tumor volume reduction after 2 or more years of treatment) participated in the study. Interventions: After withdrawal, if prolactin returned above reference range, another measurement was obtained within 1 month, symptoms were assessed by questionnaire, and magnetic resonance imaging was performed. Main Outcome Measures: We measured risk of and time to recurrence estimates as well as clinical predictors of recurrence. Results: Mean age of patients was 50 ± 13 yr, and 70% were women. Thirty-one patients had microprolactinomas, 11 had macroprolactinomas, and four had nontumoral hyperprolactinemia. The overall recurrence was 54%, and the estimated risk of recurrence by 18 months was 63%. The median time to recurrence was 3 months (range, 1–18 months), with 91% of recurrences occurring within 1 yr after discontinuation. Size of tumor remnant prior to withdrawal predicted recurrence [18% increase in risk for each millimeter (95% confidence interval, 3–35; P = 0.017)]. None of the tumors enlarged in the patients experiencing recurrence, and 28% had symptoms of hypogonadism. Conclusions: Cabergoline withdrawal is practical and safe in a subset of patients as defined by The Pituitary Society guidelines; however, the average risk of long-term recurrence in our study was over 60%. Close follow-up remains important, especially within the first year. PMID:19336508

  15. Evaluation of layer type chickens under reciprocal recurrent selection

    African Journals Online (AJOL)

    Matings within pure male and female lines, and between pure male and female lines were carried out to produce purebred male line (AA x AA), female line (BB x BB), cross (AA x BB) and reciprocal cross (BB x AA). A total number of 364 pullets arising from 207 hens and 23 cocks in generation 1 and 440 pullets from 70 ...

  16. A strategy for male sterility facilitated recurrent selection in peach

    Science.gov (United States)

    Commercial peach is a highly homozygous self-compatible species with limited diversity. The incorporation of exotic germplasm and breeding of new varieties is hindered by the labor required to generate large segregating populations. Hand pollinations are characterized by a high failure rate with onl...

  17. Recurrent priapism from therapeutic quetiapine.

    Science.gov (United States)

    Saghafi, Omeed; Kao, Amanda; Druck, Jeffrey

    2014-02-01

    Priapism is rarely related to use of non-erectile related medications. The objective was to educate about the multiple possible causes of priapism and to provide treatment recommendations for the different types of priapism. We present the case of a 43-year-old African American male with a history of schizoaffective disorder who presented to our emergency department multiple times over a three year period with priapism, each episode related to the ingestion of quetiapine. Following penile aspiration and intercavernosal injection of phenylephrine, this patient had resolution of his priapism. This case demonstrates an unusual case of recurrent priapism.

  18. Recurrent hypoglycemia in a toddler.

    Science.gov (United States)

    Cohen, Marissa; Zwiebel, Sean; Jeanmonod, Rebecca

    2015-12-01

    Idiopathic ketotic hypoglycemia is the most common cause of hypoglycemia in toddlers. This diagnosis should be considered in any hypoglycemic toddler with no prior history of abnormal growth who is developmentally normal when toxic ingestions and sepsis are inconsistent with the clinical picture. Diagnosis is important in preventing serious long-term sequelae and is made in the setting of hypoglycemia, ketonuria, and ketonemia. Therefore, checking urine and blood ketones is an essential part of the evaluation in any hypoglycemic toddler. We report the case of a 3-year-old girl with recurrent hypoglycemia secondary to idiopathic ketotic hypoglycemia.

  19. The production of Regge recurrences

    CERN Document Server

    Michael, C

    1973-01-01

    For resonance states lying on a given Regge trajectory, the two-body production mechanism as a function of the excitation of the recurrence state is discussed. A dual resonance model suggests general features for the Regge-Regge-particle coupling involved in such production. An application is made to the high energy production in pi N to pi pi N of rho , f/sub 0/ and g mesons with emphasis on the relative production cross sections, the relative t-dependences, the ratio of natural to unnatural parity exchange and the helicity dependence. (18 refs).

  20. Melhoramento do cafeeiro: XXXVIII. Observações sobre progênies do cultivar Mundo-Novo de Coffea arabica na estação experimental de Mococa Coffee breeding: XXXVIII-observation on progenies of the Mundo-Novo cultivars of Coffea arabica in the Mococa experimental station

    Directory of Open Access Journals (Sweden)

    Túlio R. Rocha

    1980-01-01

    genetic variability was noticed in the yield of the S1 progenies. The S2 MP 388-6 progeny revealed to be the highest yielding one in the experiment. The 'Mundo-Novo' progenies have a larger average tree height and diameter than the other cultivars. A positive correlation was found between tree height or tree diameter and the average yield of ripe fruits. All the highest yielding 'Mundo-Novo' progenies presented a normal rate of the weight of the ripe fruits to green coffee weight, about 6.0. The percentage of the flat seed type was about 80% for the highest yielding plants. The S1 'Mundo-Novo' progenies MP 474 and MP 452 segregated for plants with larger beans, permitting selection of high yielding coffee trees with this favorable characteristic.

  1. The Impact of Liver Graft Injury on Cancer Recurrence Posttransplantation.

    Science.gov (United States)

    Li, Chang-Xian; Man, Kwan; Lo, Chung-Mau

    2017-11-01

    Liver transplantation is the most effective treatment for selected patients with hepatocellular carcinoma. However, cancer recurrence, posttransplantation, remains to be the critical issue that affects the long-term outcome of hepatocellular carcinoma recipients. In addition to tumor biology itself, increasing evidence demonstrates that acute-phase liver graft injury is a result of hepatic ischemia reperfusion injury (which is an inevitable consequence during liver transplantation) and may promote cancer recurrence at late phase posttransplantation. The liver grafts from living donors, donors after cardiac death, and steatotic donors have been considered as promising sources of organs for liver transplantation and are associated with high incidence of liver graft injury. The acute-phase liver graft injury will trigger a series of inflammatory cascades, which may not only activate the cell signaling pathways regulating the tumor cell invasion and migration but also mobilize the circulating progenitor and immune cells to facilitate tumor recurrence and metastasis. The injured liver graft may also provide the favorable microenvironment for tumor cell growth, migration, and invasion through the disturbance of microcirculatory barrier function, induction of hypoxia and angiogenesis. This review aims to summarize the latest findings about the role and mechanisms of liver graft injury resulted from hepatic ischemia reperfusion injury on tumor recurrence posttransplantation, both in clinical and animal cohorts.

  2. Sigma Virus (DMelSV Incidence in Lines of Drosophila melanogaster Selected for Survival following Infection with Bacillus cereus

    Directory of Open Access Journals (Sweden)

    Meghan L. Bentz

    2017-01-01

    Full Text Available The immune response of Drosophila melanogaster is complex and involves both specific and general responses to parasites. In this study we tested for cross-immunity for bacteria and viruses by scoring the incidence of infection with the vertically transmitted Sigma virus (DMelSV in the progeny of a cross between females transmitting DMelSV at high frequencies and males from lines subjected to three selection regimes related to resistance to Bacillus cereus. There was no significant difference in transmission of DMelSV among selection regimes, though results suggest that the B. cereus selected lines had lower rates of infection by DMelSV. We found a significant difference in viral infection with respect to the sex of the progeny, with males consistently less likely to be infected than females. Given a finite energy budget, flies that have experienced immune system challenge may show alterations in other life history traits. Later eclosing progeny were also less likely to be infected than earlier eclosing progeny, indicating a relationship with development time. Finally, there was a significant interaction between the timing of collection and the sex of the progeny, such that later eclosing males were the most resistant group. Increased development time is sometimes associated with increased energy acquisition; from this perspective, increased development time may be associated with acquiring sufficient resources for effective resistance.

  3. Analysis of Recurrence Management in Patients Who Underwent Nonsurgical Treatment for Acute Appendicitis

    Science.gov (United States)

    Liang, Tsung-Jung; Liu, Shiuh-Inn; Tsai, Chung-Yu; Kang, Chi-Hsiang; Huang, Wei-Chun; Chang, Hong-Tai; Chen, I-Shu

    2016-01-01

    Abstract The recurrence rate for acute appendicitis treated nonoperatively varies between studies. Few studies have adequately evaluated the management of these patients when appendicitis recurs. We aimed to explore the recurrence rate and management of patients with acute appendicitis that were first treated nonoperatively. We identified patients in the Taiwan National Health Insurance Research Database who were hospitalized due to acute appendicitis for the first time between 2000 and 2010 and received nonsurgical treatment. The recurrence and its management were recorded. Data were analyzed to access the risk factors for recurrence and factors that influenced the management of recurrent appendicitis. Among the 239,821 patients hospitalized with acute appendicitis for the first time, 12,235 (5.1%) patients were managed nonoperatively. Of these, 864 (7.1%) had a recurrence during a median follow-up of 6.5 years. Appendectomy was performed by an open and laparoscopic approach in 483 (55.9%) and 258 (29.9%) patients, respectively. The remaining 123 (14.2%) patients were again treated nonsurgically. Recurrence was independently associated with young age, male sex, percutaneous abscess drainage, and medical center admission by multivariable analysis. In addition, age appendicitis, percutaneous abscess drainage, nor length of first time hospital stay had an influence on the selection of surgical approach. In conclusion, a laparoscopic appendectomy can be performed in recurrent appendicitis cases, and its application may not be related to previous appendicitis severity. PMID:27015200

  4. Psychometric properties of the Japanese version of the Concerns About Recurrence Scale (CARS-J).

    Science.gov (United States)

    Momino, Kanae; Akechi, Tatuo; Yamashita, Toshinari; Fujita, Takashi; Hayahi, Hironori; Tsunoda, Nobuyuki; Miyashita, Mitsunori; Iwata, Hiroji

    2014-05-01

    Although the fear of recurrence is a major concern among breast cancer survivors after their surgery, there are no instruments to evaluate their distress in Japan. This study examines the psychometric properties of the Japanese version of the concerns about recurrence scale, which was originally developed in the USA. The forward and backward translation method was used to develop Concerns About Recurrence Scale. Randomly selected ambulatory female patients with breast cancer participated in this study. They were asked to complete Japanese version of the concerns about recurrence scale and Hospital Anxiety and Depression Scale. The validity and reliability of Japanese version of the concerns about recurrence scale were evaluated statistically. Data were obtained from 375 patients. A novel four-factor solution was found (Health and Death Worries, Womanhood Worries, Self-valued Worries and Role Worries) that accounted for 59.2% of the total variance. Correlation coefficients between the Japanese version of the concerns about recurrence scale subscale scores and the anxiety score measured by Hospital anxiety and depression scale ranged from 0.39 to 0.60. Cronbach's alpha coefficients, which are measures of the internal consistency of the subscales, ranged from 0.86 to 0.94. The results suggest that Japanese version of the concerns about recurrence scale is a reliable and valid clinical research tool to evaluate the fear of recurrence among patients with breast cancer in Japan, although there may be cross-cultural differences regarding factor structures between Western and Japanese breast cancer patients.

  5. Fear of new or recurrent melanoma after treatment for localised melanoma.

    Science.gov (United States)

    Bell, Katy J L; Mehta, Yachna; Turner, Robin M; Morton, Rachael L; Dieng, Mbathio; Saw, Robyn; Guitera, Pascale; McCaffery, Kirsten; Low, Donald; Low, Cynthia; Jenkins, Marisa; Irwig, Les; Webster, Angela C

    2017-11-01

    To estimate the amount of fear of new or recurrent melanoma among people treated for localised melanoma in an Australian specialist centre. We randomly selected 400 potential participants from all those treated for localised melanoma at the Melanoma Institute Australia during 2014 (n = 902). They were asked to complete an adapted version of the Fear of Cancer Recurrence Inventory (FCRI). We calculated summary statistics for demographics, clinical variables and total FCRI and subscale scores. Two hundred fifteen people (54%) completed the FCRI questionnaire. The overall mean severity subscale score was 15.0 (95% CI 14.0-16.1). A high proportion of participants had scores above a proposed threshold to screen for clinical fear of cancer recurrence (77% and 63% of participants with and without new or recurrent melanoma had severity subscale scores ≥13). Most participants also had scores above a threshold found to have high specificity for clinical fear of cancer recurrence (65% and 48% of participants with and without new or recurrent melanoma had severity subscale scores ≥16). The severity subscale appeared to discriminate well between groups with differing levels of risk of new or recurrent melanoma. There is a substantial amount of fear of new or recurrent melanoma among this population, despite most having a very good prognosis. Copyright © 2017 John Wiley & Sons, Ltd.

  6. Recurrent infections and joint pain.

    Science.gov (United States)

    Bueso, Monica B; Caballero, Rosario; Castro, Henry J; Barakat, Amin; Bellanti, Joseph A

    2006-01-01

    A seven-year-old white male presented with recurrent bouts of paranasal sinusitis, streptococcal pharyngotonsillitis, lower respiratory tract infections, continuous low-grade fever, and conjunctivitis, which required frequent use of antibiotics over a period of two years. A careful review of systems also revealed a six-month history of arthralgia affecting his knees, elbows, and hands, which limited his daily activities. Prominent in the history were recurrent bouts of a generalized salmon-red, nonpruritic rash, which was most pronounced on the face and trunk and which was exacerbated by fever. His past medical history revealed severe bouts of gastroesophageal reflux disease, chronic intermittent bloody mucous diarrhea, and atopic dermatitis. A detailed review of the patient's family pedigree over five generations revealed a strong genetic predisposition for autoimmune diseases of several types. His physical examination revealed a thin, pale, chronically ill-appearing male, bilateral conjunctivitis, and pale nasal mucosae with no lymphadenopathy, organomegaly, arthritis, or rash. All laboratory results were unremarkable except for a positive rheumatoid factor and a suboptimal antibody response to immunization with pneumococcal vaccine. A diagnosis of juvenile rheumatoid arthritis of the systemic onset type was established, and, based upon his humoral immune deficiency, treatment with intravenous immunoglobulin was initiated with remarkable improvement in his symptomatology.

  7. Recurrence of Acute Duodenal Ulcer

    Directory of Open Access Journals (Sweden)

    RJ Bailey

    1994-01-01

    Full Text Available Nizatidine, 300 mg once nightly, was compared with cimetidine, 800 mg once nightly, for treatment of 212 adult out-patients with acute duodenal ulcers in an eight-week randomized, double-blind, multicentre trial. Patients were endoscoped at weeks 2, 4 and 8, regardless of ulcer healing status. No significant differences in ulcer healing rates between treatment groups were seen at weeks 2 and 4, but at week 8, nizatidine had a significantly higher ulcer healing rate (P=0.036 than cimetidine (86% versus 74%, respectively. Patients with healed ulcers at either week 2 or week 4 had a final endoscopy performed at week 8. The rate of ulcer recurrence was significantly greater (P=0.021 in the cimetidine group at week 8 compared with the nizatidine group: 21% versus 7.3%, respectively. Increasing tolerance to H2 receptor antagonist therapy with prolonged use may explain the higher recurrence rate of cimetidine. Both drugs provided equally rapid and effective symptomatic relief from epigastric pain after two weeks of therapy. Both were equally safe and free from treatment-related adverse effects.

  8. [Treatment of recurrent Dupuytren's disease].

    Science.gov (United States)

    Pillukat, T; Walle, L; Stüber, R; Windolf, J; van Schoonhoven, J

    2017-04-01

    The tendency of recurrence or progression is a frequent problem in Dupuytren's disease. The management of recurrence is adapted to the individual situation and the patient's needs. In mild cases a non-operative approach is recommended. Revision surgery is reserved for disabling situations with acceptable circulation and sensation in absence of dystrophy. It is complicated by a combined formation of scar tissue and new cords. This increases the risk of soft tissue loss and injuries to the neurovascular bundles, which impair sensation and circulation and may result in loss of the finger. The strategy consists of preoperative planning of the soft tissue reconstruction, meticulous preparation of the neurovascular bundles, arthrolyses and skin closure by Z‑plasty or transposition flaps. The corrective arthrodesis of the proximal interphalangeal joint may be an alternative to improve function without the risks of revision surgery. In cases of severe impaired circulation, sensation or dystrophy of the finger, amputation or ray resection may be indicated.

  9. Soft tissue recurrence of giant cell tumor of the bone: Prevalence and radiographic features

    Directory of Open Access Journals (Sweden)

    Leilei Xu

    2017-11-01

    Full Text Available Aim: Recurrence of giant cell tumor of bone (GCTB in the soft tissue is rarely seen in the clinical practice. This study aims to determine the prevalence of soft tissue recurrence of GCTB, and to characterize its radiographic features. Methods: A total of 291 patients treated by intralesional curettage for histologically diagnosed GCTB were reviewed. 6 patients were identified to have the recurrence of GCTB in the soft tissue, all of whom had undergone marginal resection of the lesion. Based on the x-ray, CT and MRI imaging, the radiographic features of soft tissue recurrence were classified into 3 types. Type I was defined as soft tissue recurrence with peripheral ossification, type II was defined as soft tissue recurrence with central ossification, and type III was defined as pure soft tissue recurrence without ossification. Demographic data including period of recurrence and follow-up duration after the second surgery were recorded for these 6 patients. Musculoskeletal Tumor Society (MSTS scoring system was used to evaluate functional outcomes. Results: The overall recurrence rate was 2.1% (6/291. The mean interval between initial surgery and recurrence was 11.3 ± 4.1 months (range, 5–17. The recurrence lesions were located in the thigh of 2 patients, in the forearm of 2 patients and in the leg of the other 2 patients. According to the classification system mentioned above, 2 patients were classified with type I, 1 as type II and 3 as type III. After the marginal excision surgery, all patients were consistently followed up for a mean period of 13.4 ± 5.3 months (range, 6–19, with no recurrence observed at the final visit. All the patients were satisfied with the surgical outcome. According to the MSTS scale, the mean postoperative functional score was 28.0 ± 1.2 (range, 26–29. Conclusions: The classification of soft tissue recurrence of GCTB may be helpful for the surgeon to select the appropriate imaging procedure to

  10. Investigation of the exposure to radon and progeny in the thermal spas of Loutraki (Attica-Greece): results from measurements and modelling.

    Science.gov (United States)

    Nikolopoulos, Dimitrios; Vogiannis, Efstratios; Petraki, Ermioni; Zisos, Athanasios; Louizi, Anna

    2010-01-01

    Radon and progeny ((218)Po, (214)Pb, (214)Bi and (214)Po) in thermal spas are well known radioactive pollutants identified for additional radiation burden of patients due to the activity concentration peaks which appear during bath treatment or due to drinking of waters of high radon content. This burden affects additionally the working personnel of the spas. The present paper has focused on the thermal spas of Loutraki (Attica-Greece). The aim was the investigation of the health impact for patients and working personnel due to radon and progeny. Attention has been paid to radon and progeny transient concentration peaks (for bath treatment) and to radon of thermal waters (both for bath treatment and drinking therapy). Designed experiments have been carried out, which included radon and progeny activity concentration measurements in thermal waters and ambient air. Additionally, published models for description of radon and progeny transient concentration peaks were employed. The models were based on physicochemical processes involved and employed non linear first order derivative mass balance differential equations which were solved numerically with the aid of specially developed computer codes. The collected measurements were analysed incorporating these models. Results were checked via non linear statistical tests. Predictions and measurements were found in close agreement. Non linear parameters were estimated. The models were employed for dosimetric estimations of patients and working personnel. The effective doses of patients receiving bath treatment were found low but not negligible. The corresponding doses to patients receiving potable treatment were found high but below the proposed international limits. It was found that the working personnel are exposed to considerable effective doses, however well below the acceptable limits for workers. It was concluded that treatment and working in the Loutraki spas leads to intense variations of radon and progeny and

  11. Recurrent glomerular disease after kidney transplantation.

    Science.gov (United States)

    Blosser, Christopher D; Bloom, Roy D

    2017-11-01

    With improving short-term kidney transplant outcomes, recurrent glomerular disease is being increasingly recognized as an important cause of chronic allograft failure. Further understanding of the risks and pathogenesis of recurrent glomerular disease enable informed transplant decisions, along with the development of preventive and treatment strategies. Multiple observational studies have highlighted differences in rates and outcomes for various recurrent glomerular diseases, although these rates have not markedly improved over the last decade. Emerging evidence supports use of rituximab to treat recurrent primary membranous nephropathy and possibly focal segmental glomerulosclerosis (FSGS), whereas eculizumab is effective in glomerular diseases associated with complement dysregulation [C3 glomerulopathy (C3G) and atypical hemolytic uremic syndrome (aHUS)]. Despite the potential for recurrence in the allograft, transplant remains the optimal therapy for patients with advanced chronic kidney disease (CKD) secondary to primary glomerular disease. Biomarkers and therapeutic options necessitate accurate pretransplant diagnoses with opportunities for improved surveillance and treatment of recurrent glomerular disease posttransplant.

  12. Productivity, water-use efficiency and tolerance to moderate water deficit correlate in 33 poplar genotypes from a Populus deltoides x Populus trichocarpa F1 progeny.

    Science.gov (United States)

    Monclus, R; Villar, M; Barbaroux, C; Bastien, C; Fichot, R; Delmotte, F M; Delay, D; Petit, J-M; Bréchet, C; Dreyer, E; Brignolas, F

    2009-11-01

    Genotypic variability for productivity, water-use efficiency and leaf traits in 33 genotypes selected from an F1 progeny of Populus deltoides Bartr. ex Marsh x Populus trichocarpa L. was explored under optimal and moderate water-deficit conditions. Saplings of the 33 genotypes were grown in a two-plot open field at INRA Orléans (France) and coppiced every year. A moderate water deficit was induced during two successive years on one plot by withholding irrigation, while the second one remained irrigated (control). Stem biomass and leaf structure (e.g., specific leaf area and leaf area) were measured in 2004 and 2005 and functional leaf traits (e.g., carbon isotope discrimination, Delta) were measured only in 2004. Tolerance to water deficit was estimated at genotype level as the ability to limit losses in biomass production in water deficit versus control trees. Stem biomass, leaf structure and Delta displayed a significant genotypic variability whatever the irrigation regime. For all traits, genotype ranks remained stable across years for similar irrigation conditions. Carbon isotope discrimination scaled negatively with productivity and leaf nitrogen content in controls. The most productive genotypes were the least tolerant to moderate water deficit. No relationship was evidenced between Delta and the level of tolerance to water deficit. The relationships between traits evidenced in this collection of P. deltoides x P. trichocarpa F1 genotypes contrast with the ones that were previously detected in a collection of P. deltoides x Populus nigra L. cultivars tested in the same field trial.

  13. Recurrent generalized tetanus: a case report.

    Science.gov (United States)

    Alhaji, M A; Mustapha, M G; Ashir, G M; Akuhwa, R T; Bello, M A; Farouk, A G

    2011-04-01

    We describe recurrent generalized tetanus in a four-year-old unimmunized boy following recurrent suppurative otitis media (SOM) within an 11-month period. There are not many published reports on recurrent tetanus. We highlight the importance of both primary immunizations and the need for active immunization before discharge as the infection does not confer a lifelong immunity. The usefulness of booster doses of tetanus toxoid and missed opportunities for immunization are emphasized.

  14. Recurrent, giant subcutaneous leiomyosarcoma of the thigh

    Directory of Open Access Journals (Sweden)

    Gao Chuanping, MD

    2015-10-01

    Full Text Available We present a case of recurrent, massive subcutaneous leiomyosarcoma involving the left thigh in a 29-year-old male from Madagascar. The patient had earlier undergone local resection of subcutaneous leiomyosarcoma a half year before. After surgical intervention, local recurrence developed at this site and was rapidly growing. The patient was surgically treated with a 2-cm-wide margin local excision in our hospital. The patient has remained recurrence free at 1-year follow-up.

  15. Recurrence of anxiety disorders and its predictors.

    Science.gov (United States)

    Scholten, Willemijn D; Batelaan, Neeltje M; van Balkom, Anton Jlm; Wjh Penninx, Brenda; Smit, Johannes H; van Oppen, Patricia

    2013-05-01

    The chronic course of anxiety disorders and its high burden of disease are partly due to the recurrence of anxiety disorders after remission. However, knowledge about recurrence rates and predictors of recurrence is scarce. This article reports on recurrence rates of anxiety disorders and investigates predictors of recurrence from a broad range of socio-demographic characteristics, illness-related and psychosocial putative predictors. Baseline and 2-year follow-up data were derived from the Netherlands Study of Depression and Anxiety (NESDA). Participants who had at least one lifetime anxiety disorder (panic disorder with or without agoraphobia, agoraphobia alone, social phobia or generalized anxiety disorder), but were remitted at baseline (N=429) were included. Recurrence of anxiety disorders during the 2-year follow-up period was assessed using the Composite International Diagnostic Interview, version 2.1. Recurrence rates among pure and multiple anxiety disorders did not differ significantly and the overall recurrence rate of anxiety disorders was 23.5%. In those recurring, the incidence of a new anxiety disorder was common (32.7%). Disability and anxiety sensitivity remained predictive of recurrence of anxiety disorders in multivariable regression analysis. The included participants had more severe symptoms at baseline than the non-response group and lifetime anxiety diagnoses were assessed, retrospectively. Recurrence of anxiety disorders is common and clinicians should be aware of the diagnostic instability within anxiety disorders. Disability and anxiety sensitivity are independent predictors of recurrence of anxiety disorders. Altering these predictors in regular cognitive behavioural therapy could contribute to the reduction of recurrence. Copyright © 2012 Elsevier B.V. All rights reserved.

  16. Expected gain in selection of Pinus elliottii var. elliottii for timber production at an early age

    Directory of Open Access Journals (Sweden)

    Juliana Prado Moreira

    2014-07-01

    Full Text Available P. elliotii var. elliottii is the second most planted fast growing tree species in Brazilian subtropics for wood and resin production. The objective of this work was to predict genetic values of fast growing individual trees. A trial was established in a randomized complete blocks design involving 76 treatments, 75 open pollinated progenies from a clonal seed orchard and a commercial progeny as control, in a 3 m x 3 m spacing. Total heights of all individuals were measured at 1, 2, and 3 years old and also stem diameter at 1.3 m height- dbh at 3 years old. Deviance analysis was performed by using Selegen-REML/BLUP software and genetic parameters were estimated. Significant variation was detected among progenies. Estimates of narrow-sense individual heritabilities were 0.25 and 0.42, respectively, for height and dbh. Some progenies grew faster than the control (commercial progeny and the observed genetic variation was sufficiently high to encourage selection to obtain genetic gains in wood production.

  17. The effect of a non-starch polysaccharide-hydrolysing enzyme (Rovabio® Excel) on feed intake and body condition of sows during lactation and on progeny growth performance.

    Science.gov (United States)

    Walsh, M C; Geraert, P A; Maillard, R; Kluess, J; Lawlor, P G

    2012-10-01

    A total of 200 (Large White × Landrace) sows were used in a 39-day study to evaluate the effects of feeding a non-starch polysaccharide (NSP)-hydrolysing enzyme multicomplex (Rovabio(®) Excel) in conjunction with a high- or reduced nutrient-density diet during lactation on sow body condition, feed intake and progeny performance. Eight sows were selected each week for 25 weeks, blocked by parity and BW into groups of four, and within the block randomly assigned to one of the four treatments (n = 50/treatment). Treatments were: (1) LND: low energy (13.14 MJ of DE/kg), low CP (15%) diet; (2) LND + RE: LND with 50 mg/kg NSP-hydrolysing enzyme; (3) HND: high energy (14.5 MJ of DE/kg), high CP (16.5%) diet; and (4) HND + RE: HND with 50 mg/kg NSP-hydrolysing enzyme. Sows were fed treatment diets from day 109 of gestation until the day of subsequent service. Between weaning and re-service, Rovabio(®) Excel addition to LND diets resulted in an increase in energy intake; however, a reduction was observed when supplemented to the HND diet (P Excel increased feed and energy intake during week 3 (days 15 to 21) of lactation (P Excel had greater back-fat depth at weaning and service (P < 0.05); however, the magnitude of change in back-fat depth during lactation and from farrowing to service was not different between treatments. Feeding the HND diet increased energy intake before farrowing, throughout lactation and during the weaning to service interval (P < 0.01); however, overall, average daily feed intake tended to be reduced (P < 0.10). At service, sows fed the HND diet were heavier than sows fed the LND diet (P < 0.05); however, the magnitude of change in BW between treatments was not different. Feeding the HND diet to sows resulted in a tendency for heavier piglets at birth (P = 0.10) that tended to grow at a faster rate and be heavier at weaning than piglets from sows fed the LND diet (P = 0.06). These results indicate that NSP-degrading enzymes offer minimal benefit

  18. Prepartum dietary energy source fed to beef cows: II. Effects on progeny postnatal growth, glucose tolerance, and carcass composition.

    Science.gov (United States)

    Radunz, A E; Fluharty, F L; Relling, A E; Felix, T L; Shoup, L M; Zerby, H N; Loerch, S C

    2012-12-01

    Mature Angus-cross beef cows (n = 228) were used to evaluate effects of prepartum dietary energy source on postnatal growth and carcass composition of progeny in a 2-yr study. Starting at approximately 160 d of gestation, cows were fed diets consisting of 1 of 3 primary energy sources: grass hay (HY), corn (CN), or dried corn distillers grains with solubles (DG). The CN and DG diets were limit-fed to achieve similar energy intakes as cows fed HY. Following parturition, cows were fed a common diet and managed as a single group. Calves were weaned at an average of 185 ± 6 d of age and backgrounded for 28 d. A subset of progeny (n = 134) was individually fed a common finishing diet until slaughter, when each calf reached 1.2 ± 0.05 cm of backfat. A glucose tolerance test (GTT) was conducted in year 2 on 4 calves/treatment after 41 and 111 d on the finishing diet (DOF). Calf birth weights were greater (P = 0.002) in calves from cows fed CN and DG than calves from cows fed HY, and weaning BW (P = 0.08) was less for calves from cows fed HY vs. CN. Receiving BW, final BW, and HCW did not differ (P ≥ 0.16) among treatments. No difference (P ≥ 0.28) in ADG, morbidity, and mortality from birth to slaughter was observed among treatments. In response to a GTT, increased DOF resulted in greater (P ≤ 0.005) fasting insulin, faster glucose disappearance rate, and greater insulin:glucose area under the curve ratio. Glucose disappearance rate was greater (P = 0.01) in calves from cows fed CN than in calves from cows fed HY or DG. A greater initial insulin response (P = 0.005) was observed in calves from cows fed CN or DG than in calves from cows fed HY. Carcass traits used to measure yield grade did not differ (P ≥ 0.19) among treatments. Calves from dams fed CN had the lowest marbling score (P = 0.03) and intramuscular fat content (P = 0.07). These results indicate that prepartum maternal dietary energy source can alter fetal adipose tissue development and insulin

  19. Zebrafish exposure to environmentally relevant concentration of depleted uranium impairs progeny development at the molecular and histological levels.

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    Olivier Armant

    Full Text Available Uranium is an actinide naturally found in the environment. Anthropogenic activities lead to the release of increasing amounts of uranium and depleted uranium (DU in the environment, posing potential risks to aquatic organisms due to radiological and chemical toxicity of this radionucleide. Although environmental contaminations with high levels of uranium have already been observed, chronic exposures of non-human species to levels close to the environmental quality standards remain scarcely characterized. The present study focused on the identification of the molecular pathways impacted by a chronic exposure of zebrafish to 20 μg/L of DU during 10 days. The transcriptomic effects were evaluated by the use of the mRNAseq analysis in three organs of adult zebrafish, the brain the testis and the ovaries, and two developmental stages of the adult fish progeny, two-cells embryo and four-days larvae. The results highlight generic effects on the cell adhesion process, but also specific transcriptomic responses depending on the organ or the developmental stage investigated. The analysis of the transgenerational effects of DU-exposure on the four-day zebrafish larvae demonstrate an induction of genes involved in oxidative response (cat, mpx, sod1 and sod2, a decrease of expression of the two hatching enzymes (he1a and he1b, the deregulation of the expression of gene coding for the ATPase complex and the induction of cellular stress. Electron microscopy analysis of skeletal muscles on the four-days larvae highlights significant histological impacts on the ultrastructure of both the mitochondria and the myofibres. In addition, the comparison with the transcriptomic data obtained for the acetylcholine esterase mutant reveals the induction of protein-chaperons in the skeletal muscles of the progeny of fish chronically exposed to DU, pointing towards long lasting effects of this chemical in the muscles. The results presented in this study support the

  20. Recurrence and Relapse in Bipolar Mood Disorder

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    S Gh Mousavi

    2004-06-01

    Full Text Available Background: Despite the effectiveness of pharmacotherapy in acute phase of bipolar mood disorder, patients often experience relapses or recurrent episodes. Hospitalization of patients need a great deal of financial and humanistic resources which can be saved through understanding more about the rate of relapse and factors affecting this rate. Methods: In a descriptive analytical study, 380 patients with bipolar disorder who were hospitalized in psychiatric emergency ward of Noor hospital, Isfahan, Iran, were followed. Each patient was considered for; the frequency of relapse and recurrence, kind of pharmachotherapy, presence of psychotherapeutic treatments, frequency of visits by psychiatrist and the rank of present episode. Results: The overall prevalence of recurrence was 42.2%. Recurrence was lower in patients using lithium carbonate or sodium valproate or combined therapy (about 40%, compared to those using carbamazepine (80%. Recurrence was higher in patients treated with only pharmacotherapy (44.5% compared to those treated with both pharmacotherapy and psychotherapy (22.2%. Patients who were visited monthy by psychiatrist had lower rate of recurrence compared to those who had irregular visits. Conclusion: The higher rate of recurrence observed in carbamazepine therapy may be due to its adverse reactions and consequently poor compliance to this drug. Lower rates of recurrence with psychotherapy and regular visits may be related to the preventive effects of these procedures and especially to the effective management of stress. Keywords: Bipolar Mood Disorder, Recurrence, Relapse.

  1. Unique double recurrence of cerebral arteriovenous malformation.

    Science.gov (United States)

    Nagm, Alhusain; Horiuchi, Tetsuyoshi; Ichinose, Shunsuke; Hongo, Kazuhiro

    2015-09-01

    Surgically treated patients with arteriovenous malformations (AVMs) are considered cured when the postoperative angiogram proves complete resection. However, despite no residual nidus or early draining vein on postoperative angiogram, rare instances of AVM recurrence have been reported in adults. In this paper, the authors present a case of a 24-year-old woman with asymptomatic double recurrence of her cerebral AVM after angiographically proven complete resection. To the authors' knowledge, this patient represents the first case with double de novo asymptomatic recurrence of Spetzler-Martin grade I AVM. Also, she represents the first case with unique AVM criteria in each recurrence.

  2. Palbociclib Isethionate in Treating Younger Patients With Recurrent, Progressive, or Refractory Central Nervous System Tumors

    Science.gov (United States)

    2017-09-27

    Childhood Choroid Plexus Tumor; Childhood Ependymoblastoma; Childhood Grade III Meningioma; Childhood High-grade Cerebellar Astrocytoma; Childhood High-grade Cerebral Astrocytoma; Childhood Medulloepithelioma; Recurrent Childhood Anaplastic Astrocytoma; Recurrent Childhood Anaplastic Oligoastrocytoma; Recurrent Childhood Anaplastic Oligodendroglioma; Recurrent Childhood Brain Stem Glioma; Recurrent Childhood Cerebellar Astrocytoma; Recurrent Childhood Cerebral Astrocytoma; Recurrent Childhood Giant Cell Glioblastoma; Recurrent Childhood Glioblastoma; Recurrent Childhood Gliomatosis Cerebri; Recurrent Childhood Gliosarcoma; Recurrent Childhood Medulloblastoma; Recurrent Childhood Pineoblastoma; Recurrent Childhood Supratentorial Primitive Neuroectodermal Tumor

  3. Genetic parameters and selection strategies for soybean genotypes resistant to the stink bug-complex.

    Science.gov (United States)

    de Godoi, Cláudio Roberto Cardoso; Pinheiro, José Baldin

    2009-04-01

    Soybean genotypes resistant to stink bugs are derived from complex breeding processes obtained through indirect selection. The aim of the present work was to estimate genetic parameters for guiding selection strategies towards resistant genotypes, based on those traits associated with responses to pod-attacking stink bugs, such as the grain filling period (GFP), leaf retention (LR), percentage index of pod damage (PIPD) and percentage of spotted seeds (PSS). We assessed the parental lines IAC-100 (resistant) and FT-Estrela (susceptible), the progenies F(2) and F (4) , 30 progenies F (2:3) , 30 progenies BC (1) F (2:3) and 30 progenies BC (2) F (2:3) , besides the cultivars BRS Celeste and MGBR-46 (Conquista). Three field experiments, using randomized complete block design with three replications, were installed in Goiânia-GO, in the 2002/03 season. Each experiment consisted of 36 treatments (6 common and 30 regular). Heritability estimates were: 74.6 and 36.1 (GFP); 51.9 and 19.9 (LR); 49.6 and 49.6 (PIPD) and 55.8 and 20.3 (PSS), in both the broad and narrow senses, respectively. Based on these results, we concluded that the best strategy for obtaining stink bug-resistant genotypes consists of selecting the PIPD trait in early generations (F (3) or F (4) ), followed by selection for the GFP, LR and PSS traits in generations with higher endogamy levels.

  4. Selecting honey bees for worker brood that reduces the reproduction of Varroa destructor

    Science.gov (United States)

    We investigated an effect of Apis mellifera worker brood on the reproduction of Varroa destructor as a resistance trait by conducting seven generations of bidirectional selection. Initial tests showed two-fold differences in mite fecundity (progeny per foundress mites) between colonies of different...

  5. Genetic parameters and selection strategies for soybean genotypes resistant to the stink bug-complex

    Science.gov (United States)

    2009-01-01

    Soybean genotypes resistant to stink bugs are derived from complex breeding processes obtained through indirect selection. The aim of the present work was to estimate genetic parameters for guiding selection strategies towards resistant genotypes, based on those traits associated with responses to pod-attacking stink bugs, such as the grain filling period (GFP), leaf retention (LR), percentage index of pod damage (PIPD) and percentage of spotted seeds (PSS). We assessed the parental lines IAC-100 (resistant) and FT-Estrela (susceptible), the progenies F2 and F 4 , 30 progenies F 2:3 , 30 progenies BC 1 F 2:3 and 30 progenies BC 2 F 2:3 , besides the cultivars BRS Celeste and MGBR-46 (Conquista). Three field experiments, using randomized complete block design with three replications, were installed in Goiânia-GO, in the 2002/03 season. Each experiment consisted of 36 treatments (6 common and 30 regular). Heritability estimates were: 74.6 and 36.1 (GFP); 51.9 and 19.9 (LR); 49.6 and 49.6 (PIPD) and 55.8 and 20.3 (PSS), in both the broad and narrow senses, respectively. Based on these results, we concluded that the best strategy for obtaining stink bug-resistant genotypes consists of selecting the PIPD trait in early generations (F 3 or F 4 ), followed by selection for the GFP, LR and PSS traits in generations with higher endogamy levels. PMID:21637688

  6. Genetic parameters and selection strategies for soybean genotypes resistant to the stink bug-complex

    Directory of Open Access Journals (Sweden)

    Cláudio Roberto Cardoso de Godoi

    2009-01-01

    Full Text Available Soybean genotypes resistant to stink bugs are derived from complex breeding processes obtained through indirect selection. The aim of the present work was to estimate genetic parameters for guiding selection strategies towards resistant genotypes, based on those traits associated with responses to pod-attacking stink bugs, such as the grain filling period (GFP, leaf retention (LR, percentage index of pod damage (PIPD and percentage of spotted seeds (PSS. We assessed the parental lines IAC-100 (resistant and FT-Estrela (susceptible, the progenies F2 and F4, 30 progenies F2:3, 30 progenies BC1F2:3 and 30 progenies BC2F2:3, besides the cultivars BRS Celeste and MGBR-46 (Conquista. Three field experiments, using randomized complete block design with three replications, were installed in Goiânia-GO, in the 2002/03 season. Each experiment consisted of 36 treatments (6 common and 30 regular. Heritability estimates were: 74.6 and 36.1 (GFP; 51.9 and 19.9 (LR; 49.6 and 49.6 (PIPD and 55.8 and 20.3 (PSS, in both the broad and narrow senses, respectively. Based on these results, we concluded that the best strategy for obtaining stink bug-resistant genotypes consists of selecting the PIPD trait in early generations (F3 or F4, followed by selection for the GFP, LR and PSS traits in generations with higher endogamy levels.

  7. Molecular variability from two selection of BRT10 population in an ...

    African Journals Online (AJOL)

    Genetic variability of two groups of palms composed with four progenies selected in BRT10 improved populations resulting from successive self-fertilizations of two parents LM2T and LM10T was studied using four polymorphic microsatellites DNA markers of Elaeis guineensis Jacq. The molecular variability of those ...

  8. Diagnostic instability of recurrence and the impact on recurrence rates in depressive and anxiety disorders.

    Science.gov (United States)

    Scholten, Willemijn D; Batelaan, Neeltje M; Penninx, Brenda W J H; van Balkom, Anton J L M; Smit, Johannes H; Schoevers, Robert A; van Oppen, Patricia

    2016-05-01

    Despite increasing evidence for the diagnostic instability between and within depressive and anxiety disorders, most studies report solely on the recurrence rates of the specific index disorders. Neglecting this evidence has an inherent risk of underestimating recurrence rates of depressive and anxiety disorders. This study investigates the impact of diagnostic instability of recurrence rates in depression and anxiety. Data were derived from the Netherlands Study of Depression and Anxiety (NESDA). The sample of 656 participants had a panic disorder with or without agoraphobia, agoraphobia, social phobia, generalized anxiety disorder, major depressive disorder or dysthymia, and a subsequent remission. Recurrence rates of index disorders (diagnostically stable recurrence) and newly arisen anxiety or depressive disorders (diagnostically unstable recurrence), were calculated over a 4-year follow-up period. In anxiety disorders (n=281), the recurrence rate is more than doubled, from 23.8% with a stable recurrence, to 54.8%, when diagnostically unstable recurrences are included. In depressive disorders (N=173) the recurrence rate increases from 37.6% to 49.7%, and in comorbid anxiety and depressive disorders (N=202) the diagnostically unstable recurrences increase from 54.0% to 66.3%. Attrition during follow up may have biased the results; remission was defined as absence of symptoms for 1 month; very short-term remission and recurrence patterns were not assessed. Diagnostically unstable recurrences have a significant impact on recurrence rates, with the greatest instability for anxiety disorders. When only diagnostically stable recurrences are assessed, recurrence rates are highly underrated and provide biased estimates of the true course of these disorders. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. Recurrent Priapism from Therapeutic Quetiapine

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    Omeed Saghafi

    2014-02-01

    Full Text Available Priapism is rarely related to use of non-erectile related medications. The objective was to educate about the multiple possible causes of priapism and to provide treatment recommendations for the different types of priapism. We present the case of a 43 year-old African American male with a history of schizoaffective disorder who presented to our emergency department multiple times over a three year period with priapism, each episode related to the ingestion of quetiapine. Following penile aspiration and intercavernosal injection of phenylephrine, this patient had resolution of his priapism. This case demonstrates an unusual case of recurrent priapism. [West J Emerg Med. 2014;15(1:114–116.

  10. Recurrent Primary Spinal Hydatid Cyst

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    Okan Turk

    2015-03-01

    Full Text Available Primary hydatid disease of spine is rare and spinal hydatitosis constitute only 1% of all hydatitosis. We report a case of recurrent primary intraspinal extradural hydatid cyst of the thoracic region causing progressive paraparesis. The patient was operated 16 years ago for primary spinal hydatid disease involvement and was instrumented dorsally for stabilization. The magnetic resonance imaging (MRI of thoracic spine showed a cystic lesion at T11-12 level and compressed spinal cord posterolaterally. Intraspinal cyst was excised through T11-12 laminectomy which made formerly. The early postoperative period showed a progressive improvement of his neurological deficit and he was discharged with antihelmintic treatment consisting of albendazole and amoxicillin-sulbactam combination. [Cukurova Med J 2015; 40(Suppl 1: 84-89

  11. Recurrent pregnancy loss and obesity.

    Science.gov (United States)

    Sugiura-Ogasawara, Mayumi

    2015-05-01

    Recurrent pregnancy loss (RPL) was defined as two or more miscarriages. Antiphospholipid syndrome, uterine anomalies, and parental chromosomal abnormalities, particularly translocation and abnormal embryonic karyotype, are identifiable causes of RPL. Obesity may increase the risk of sporadic miscarriage in pregnancies conceived spontaneously. Obesity with body mass index (BMI)>30 kg/m2 is an independent risk factor for further miscarriage with odds ratio 1.7-3.5 in patients with early RPL. Obesity is associated with euploid miscarriage. Unexplained RPL with euploid embryo might be a common disease caused by both polymorphisms of multiple susceptibility genes and lifestyle factors such as women's age, obesity, and smoking. Patients with a history of RPL were found to have a higher risk of cardiovascular disease, celiac disease, gastric ulcer, gastritis, and atopic dermatitis. No study has examined the effect of weight loss on the prevention of further miscarriage in patients with RPL. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Placental apoptosis in recurrent miscarriage

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    Tarek A. Atia

    2017-09-01

    Full Text Available Apoptosis is an interactive and dynamic biological process involved in all phases of embryogenesis. We aimed to study the effect of placental apoptosis on recurrent miscarriage (RM. Placental tissue samples were collected from 40 women with RM (study group and 30 women with sporadic spontaneous abortion (control group. Samples were prepared and stained immunohistochemically with markers for both the apoptotic protein (p53 and anti-apoptotic Bcl-2 antibodies. Our results showed that expression of the apoptotic (p53 protein was significantly increased in the placental tissues of the RM group (p = 0.003. By contrast, the expression of anti-apoptotic (Bcl-2 antibodies was significantly increased in the placental tissues of the control group (p = 0.025. We concluded that placental apoptosis plays a crucial role in pregnancy continuation. However, increased p53 expression in placental tissue in early pregnancy could negatively affect pregnancy continuation.

  13. A supramolecular look at microenvironmental regulation of limbal epithelial stem cells and the differentiation of their progeny

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    Marcela Aldrovani

    Full Text Available ABSTRACT Various approaches have been taken to improve our knowledge of the microenvironmental regulation of limbal epithelial stem cells. Researchers have extensively investigated the roles of growth factors, survival factors, cytokines, enzymes, and permeable molecules secreted by the limbal cells. However, recent evidence suggests that stem cell fate (i.e., self-renewal or differentiation can also be influenced by biophysical and mechanical cues related to the supramolecular organization and the liquid crystalline (mesophase nature of the stromal extracellular matrix. These cues can be sensed by stem cells and transduced into intracellular biochemical and functional responses, a process known as mechanotransduction. The objective of this review is to offer perspectives on the supramolecular microenvironmental regulation of limbal epithelial stem cells and the differentiation of their progeny.

  14. A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation

    Directory of Open Access Journals (Sweden)

    Brian G. Ballios

    2015-06-01

    Full Text Available The utility of stem cells and their progeny in adult transplantation models has been limited by poor survival and integration. We designed an injectable and bioresorbable hydrogel blend of hyaluronan and methylcellulose (HAMC and tested it with two cell types in two animal models, thereby gaining an understanding of its general applicability for enhanced cell distribution, survival, integration, and functional repair relative to conventional cell delivery in saline. HAMC improves cell survival and integration of retinal stem cell (RSC-derived rods in the retina. The pro-survival mechanism of HAMC is ascribed to the interaction of the CD44 receptor with HA. Transient disruption of the retinal outer limiting membrane, combined with HAMC delivery, results in significantly improved rod survival and visual function. HAMC also improves the distribution, viability, and functional repair of neural stem and progenitor cells (NSCs. The HAMC delivery system improves cell transplantation efficacy in two CNS models, suggesting broad applicability.

  15. Intratumoural evolutionary landscape of high-risk prostate cancer: The PROGENY study of genomic and immune parameters

    DEFF Research Database (Denmark)

    Linch, M.; Goh, G.; Hiley, C.

    2017-01-01

    Background: Intratumoural heterogeneity (ITH) is well recognised in prostate cancer (PC), but its role in high-risk disease is uncertain. A prospective, single-arm, translational study using targeted multiregion prostate biopsies was carried out to study genomic and T-cell ITH in clinically high......-risk PC aiming to identify drivers and potential therapeutic strategies. Patients and methods: Forty-nine men with elevated prostate-specific antigen and multiparametric-magnetic resonance imaging detected PC underwent image-guided multiregion transperineal biopsy. Seventy-nine tumour regions from 25....../β-catenin mutations demonstrated a low CD8+/FOXP3+ ratio, a potential surrogate marker of immune evasion. Conclusions: The PROGENY (PROstate cancer GENomic heterogeneitY) study provides a diagnostic platform suitable for studying tumour ITH. Genetic aberrations in clinically high-risk PC