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Sample records for prog rev grp

  1. 16oct AM poster prog rev.pmd

    Indian Academy of Sciences (India)

    Administrator

    1530 T V Ramakrishnan. T V Ramakrishnan. T V Ramakrishnan, BHU, Varanasi. Walter Kohn and the rise of condensed matter physics. 1600 Swapan K Ghosh. Swapan K Ghosh. Swapan K Ghosh, BARC, Mumbai. Density functional theory for materials modeling at different length scales: A legacy of Walter Kohn.

  2. Compressive Strength of Longitudinally Stiffened GRP Panels

    DEFF Research Database (Denmark)

    Böhme, J.; Noury, P.; Riber, Hans Jørgen

    1996-01-01

    A structural analysis of a cross stiffened orthotropic GRP panel subjected to uniaxial compressive loads is carried out. Analytical solutions to the buckling of such structures are proposed and validated by a finite element analysis. Both analytical and finite element approaches confirm an identi...

  3. LIMIT STRESS SPLINE MODELS FOR GRP COMPOSITES

    African Journals Online (AJOL)

    ES OBE

    INTRODUCTION. The strength of any material used in any design is very important in order to evaluate the performance index of a particular project. Plastics are polymers that are viscoelastic in nature, show time dependence response to applied stress (Creep), [1]. GRP mechanical properties are therefore affected by creep.

  4. Grp78 promotes the invasion of hepatocellular carcinoma

    Directory of Open Access Journals (Sweden)

    Li Hongdan

    2010-01-01

    Full Text Available Abstract Background Glucose regulated protein 78 (Grp78 is involved in the invasion and metastasis in many human cancers including gastric cancer, breast cancer, prostate cancer. But the role of Grp78 in the invasion of human hepatocellular carcinoma has not been reported. In this article, we examined if Grp78 was associated with the invasion of hepatocellular carcinoma and explored the possible underlying mechanism. Methods The Grp78 and FAK expression levels in 44 patients with hepatocellular carcinoma were examined using immunohistochemistry. Grp78 overexpressing SMMC7721 cells were established by pcDNA3.1 (+-Grp78 transfection and screened by G418. Grp78 and FAK levels in Grp78 overexpressing cells were down-regulated by siRNA transfection. The invasion status of tumor cells was evaluated by transwell assay in vitro, and chick embryo metastasis model in vivo. Cell spreading was determined by cell spreading assay, and quantitatively measured by Orisis software HUG. Grp78, pY397 FAK, pY576/577 FAK and FAK levels were detected by western blot. RhoA activity was detected by GST pulldown assay. The distribution of actin cytoskeleton was observed by fluorescent staining. Results Grp78 expression levels in 44 patients with hepatocellular carcinoma were negatively correlated with tumor grading, and positively correlated with portal invasion and intra-hepatic invasion. Overexpression of Grp78 in SMMC7721 cells promoted the invasion of cancer cells in vitro and in vivo, and this increase in tumor cell invasion was blocked by Grp78 siRNA knockdown. Our results also revealed that overexpression of Grp78 in SMMC7721 cells accelerated the process of cell spreading and promoted lamellipodia formation. Further analysis showed that overexpression of Grp78 in SMMC7721 cells increased pY397 and pY576/577 levels of FAK. Grp78 siRNA knockdown decreased FAK activation and activity. Our results also revealed that Grp78 overexpression in SMMC7721 cells decreased

  5. Grp78 promotes the invasion of hepatocellular carcinoma

    International Nuclear Information System (INIS)

    Su, Rongjian; Li, Zhen; Li, Hongdan; Song, Huijuan; Bao, Cuifen; Wei, Jia; Cheng, Liufang

    2010-01-01

    Glucose regulated protein 78 (Grp78) is involved in the invasion and metastasis in many human cancers including gastric cancer, breast cancer, prostate cancer. But the role of Grp78 in the invasion of human hepatocellular carcinoma has not been reported. In this article, we examined if Grp78 was associated with the invasion of hepatocellular carcinoma and explored the possible underlying mechanism. The Grp78 and FAK expression levels in 44 patients with hepatocellular carcinoma were examined using immunohistochemistry. Grp78 overexpressing SMMC7721 cells were established by pcDNA3.1 (+)-Grp78 transfection and screened by G418. Grp78 and FAK levels in Grp78 overexpressing cells were down-regulated by siRNA transfection. The invasion status of tumor cells was evaluated by transwell assay in vitro, and chick embryo metastasis model in vivo. Cell spreading was determined by cell spreading assay, and quantitatively measured by Orisis software HUG. Grp78, pY397 FAK, pY576/577 FAK and FAK levels were detected by western blot. RhoA activity was detected by GST pulldown assay. The distribution of actin cytoskeleton was observed by fluorescent staining. Grp78 expression levels in 44 patients with hepatocellular carcinoma were negatively correlated with tumor grading, and positively correlated with portal invasion and intra-hepatic invasion. Overexpression of Grp78 in SMMC7721 cells promoted the invasion of cancer cells in vitro and in vivo, and this increase in tumor cell invasion was blocked by Grp78 siRNA knockdown. Our results also revealed that overexpression of Grp78 in SMMC7721 cells accelerated the process of cell spreading and promoted lamellipodia formation. Further analysis showed that overexpression of Grp78 in SMMC7721 cells increased pY397 and pY576/577 levels of FAK. Grp78 siRNA knockdown decreased FAK activation and activity. Our results also revealed that Grp78 overexpression in SMMC7721 cells decreased RhoA-GTP level, and Grp78 siRNA knockdown rescued Rho

  6. Administrative Circulars Rev.

    CERN Multimedia

    2003-01-01

    Administrative Circular N° 19 (Rev. 3) - April 2003 Subsistence indemnity - Other expenses necessarily incurred in the course of duty travelAdministrative Circular N° 25 (Rev. 2) - April 2003 Shift work - Special provisions for the Fire and Rescue Service - These circulars have been revised. Human Resources Division Tel. 74128Copies of these circulars are available in the Divisional Secretariats. In addition, administrative and operational circulars, as well as the lists of those in force, are available for consultation on the Web at: http://humanresources.web.cern.ch/humanresources/internal/admin_services/admincirc/listadmincirc.asp

  7. Exploring the Functional Complementation between Grp94 and Hsp90.

    Directory of Open Access Journals (Sweden)

    Kevin A Maharaj

    Full Text Available Grp94 and Hsp90 are the ER and cytoplasmic paralog members, respectively, of the hsp90 family of molecular chaperones. The structural and biochemical differences between Hsp90 and Grp94 that allow each paralog to efficiently chaperone its particular set of clients are poorly understood. The two paralogs exhibit a high degree of sequence similarity, yet also display significant differences in their quaternary conformations and ATPase activity. In order to identify the structural elements that distinguish Grp94 from Hsp90, we characterized the similarities and differences between the two proteins by testing the ability of Hsp90/Grp94 chimeras to functionally substitute for the wild-type chaperones in vivo. We show that the N-terminal domain or the combination of the second lobe of the Middle domain plus the C-terminal domain of Grp94 can functionally substitute for their yeast Hsp90 counterparts but that the equivalent Hsp90 domains cannot functionally replace their counterparts in Grp94. These results also identify the interface between the Middle and C-terminal domains as an important structural unit within the Hsp90 family.

  8. Efectiveness of GrpMI with fibromyalgia patients

    DEFF Research Database (Denmark)

    Torres Serna, Esperanza

    This study attempts to demonstrate the effectiveness of Group Music and Imagery (GrpMI) with women suffering from fibromyalgia (FM). It uses a randomized controlled trial, with a pretest-posttest control group design, and a three month follow-up. The results show statistically or tendentially...... that it is advisable to use music therapy and especially Group Imagery and Music for FM treatment. The results obtained open the way for further research studies focussing on the usefulness of GrpMI in other populations that, like FM sufferers, experience chronic pain....

  9. Secondary Creep Response of Hand Lay-Up GRP Composites ...

    African Journals Online (AJOL)

    Glass Reinforced Plastics (GRP) composite load bearing components are now in common use, quite often at temperatures above the ambient, where creep behaviour may be significant, as in pressurized industrial containers. This is especially true of those composites produced by the Hand Lay-Up Contact Moulding ...

  10. A circadian clock-regulated toggle switch explains AtGRP7 and AtGRP8 oscillations in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Christoph Schmal

    Full Text Available The circadian clock controls many physiological processes in higher plants and causes a large fraction of the genome to be expressed with a 24h rhythm. The transcripts encoding the RNA-binding proteins AtGRP7 (Arabidopsis thaliana Glycine Rich Protein 7 and AtGRP8 oscillate with evening peaks. The circadian clock components CCA1 and LHY negatively affect AtGRP7 expression at the level of transcription. AtGRP7 and AtGRP8, in turn, negatively auto-regulate and reciprocally cross-regulate post-transcriptionally: high protein levels promote the generation of an alternative splice form that is rapidly degraded. This clock-regulated feedback loop has been proposed to act as a molecular slave oscillator in clock output. While mathematical models describing the circadian core oscillator in Arabidopsis thaliana were introduced recently, we propose here the first model of a circadian slave oscillator. We define the slave oscillator in terms of ordinary differential equations and identify the model's parameters by an optimization procedure based on experimental results. The model successfully reproduces the pertinent experimental findings such as waveforms, phases, and half-lives of the time-dependent concentrations. Furthermore, we obtain insights into possible mechanisms underlying the observed experimental dynamics: the negative auto-regulation and reciprocal cross-regulation via alternative splicing could be responsible for the sharply peaking waveforms of the AtGRP7 and AtGRP8 mRNA. Moreover, our results suggest that the AtGRP8 transcript oscillations are subordinated to those of AtGRP7 due to a higher impact of AtGRP7 protein on alternative splicing of its own and of the AtGRP8 pre-mRNA compared to the impact of AtGRP8 protein. Importantly, a bifurcation analysis provides theoretical evidence that the slave oscillator could be a toggle switch, arising from the reciprocal cross-regulation at the post-transcriptional level. In view of this

  11. Expression and clinical significance of Glucose Regulated Proteins GRP78 (BiP) and GRP94 (GP96) in human adenocarcinomas of the esophagus

    International Nuclear Information System (INIS)

    Langer, Rupert; Feith, Marcus; Siewert, Joerg Rüdiger; Wester, Hans-Juergen; Hoefler, Heinz

    2008-01-01

    Glucose regulated proteins (GRPs) are main regulators of cellular homeostasis due to their role as molecular chaperones. Moreover, the functions of GRPs suggest that they also may play important roles in cancer biology. In this study we investigated the glucose regulated proteins GRP78 (BiP) and GRP94 (GP96) in a series of human esophageal adenocarcinomas to determine their implications in cancer progression and prognosis. Formalin-fixed, paraffin-embedded tissues of primary resected esophageal (Barrett) adenocarcinomas (n = 137) and corresponding normal tissue were investigated. mRNA-gene expression levels of GRP78 and GRP94 were determined by quantitative real-time RT-PCR after mRNA extraction. Protein expression analysis was performed with immunohistochemical staining of the cases, assembled on a tissue micorarray. The results were correlated with pathologic features (pT, pN, G) and overall survival. GRP78 and GRP94 mRNA were expressed in all tumors. The relative gene expression of GRP78 was significantly higher in early cancers (pT1m and pT1sm) as compared to more advanced stages (pT2 and pT3) and normal tissue (p = 0.031). Highly differentiated tumors showed also higher GRP78 mRNA levels compared to moderate and low differentiated tumors (p = 0.035). In addition, patients with higher GRP78 levels tended to show a survival benefit (p = 0.07). GRP94 mRNA-levels showed no association to pathological features or clinical outcome. GRP78 and GRP94 protein expression was detectable by immunohistochemistry in all tumors. There was a significant correlation between a strong GRP78 protein expression and early tumor stages (pT1m and pT1sm, p = 0.038). For GRP94 low to moderate protein expression was significantly associated with earlier tumor stage (p = 0.001) and less lymph node involvement (p = 0.036). Interestingly, the patients with combined strong GRP78 and GRP94 protein expression exclusively showed either early (pT1m or pT1sm) or advanced (pT3) tumor stages and no

  12. Expression and clinical significance of Glucose Regulated Proteins GRP78 (BiP and GRP94 (GP96 in human adenocarcinomas of the esophagus

    Directory of Open Access Journals (Sweden)

    Wester Hans-Juergen

    2008-03-01

    Full Text Available Abstract Background Glucose regulated proteins (GRPs are main regulators of cellular homeostasis due to their role as molecular chaperones. Moreover, the functions of GRPs suggest that they also may play important roles in cancer biology. In this study we investigated the glucose regulated proteins GRP78 (BiP and GRP94 (GP96 in a series of human esophageal adenocarcinomas to determine their implications in cancer progression and prognosis. Methods Formalin-fixed, paraffin-embedded tissues of primary resected esophageal (Barrett adenocarcinomas (n = 137 and corresponding normal tissue were investigated. mRNA-gene expression levels of GRP78 and GRP94 were determined by quantitative real-time RT-PCR after mRNA extraction. Protein expression analysis was performed with immunohistochemical staining of the cases, assembled on a tissue micorarray. The results were correlated with pathologic features (pT, pN, G and overall survival. Results GRP78 and GRP94 mRNA were expressed in all tumors. The relative gene expression of GRP78 was significantly higher in early cancers (pT1m and pT1sm as compared to more advanced stages (pT2 and pT3 and normal tissue (p = 0.031. Highly differentiated tumors showed also higher GRP78 mRNA levels compared to moderate and low differentiated tumors (p = 0.035. In addition, patients with higher GRP78 levels tended to show a survival benefit (p = 0.07. GRP94 mRNA-levels showed no association to pathological features or clinical outcome. GRP78 and GRP94 protein expression was detectable by immunohistochemistry in all tumors. There was a significant correlation between a strong GRP78 protein expression and early tumor stages (pT1m and pT1sm, p = 0.038. For GRP94 low to moderate protein expression was significantly associated with earlier tumor stage (p = 0.001 and less lymph node involvement (p = 0.036. Interestingly, the patients with combined strong GRP78 and GRP94 protein expression exclusively showed either early (pT1m or p

  13. RasGRP3 regulates the migration of glioma cells via interaction with Arp3

    Science.gov (United States)

    Lee, Hae Kyung; Finniss, Susan; Cazacu, Simona; Xiang, Cunli; Poisson, Laila M.; Blumberg, Peter M.; Brodie, Chaya

    2015-01-01

    Glioblastoma (GBM), the most aggressive primary brain tumors, are highly infiltrative. Although GBM express high Ras activity and Ras proteins have been implicated in gliomagenesis, Ras-activating mutations are not frequent in these tumors. RasGRP3, an important signaling protein responsive to diacylglycerol (DAG), increases Ras activation. Here, we examined the expression and functions of RasGRP3 in GBM and glioma cells. RasGRP3 expression was upregulated in GBM specimens and glioma stem cells compared with normal brains and neural stem cells, respectively. RasGRP3 activated Ras and Rap1 in glioma cells and increased cell migration and invasion partially via Ras activation. Using pull-down assay and mass spectroscopy we identified the actin-related protein, Arp3, as a novel interacting protein of RasGRP3. The interaction of RasGRP3 and Arp3 was validated by immunofluorescence staining and co-immunoprecipitation, and PMA, which activates RasGRP3 and induces its translocation to the peri-nuclear region, increased the association of Arp3 and RasGRP3. Arp3 was upregulated in GBM, regulated cell spreading and migration and its silencing partially decreased these effects of RasGRP3 in glioma cells. In summary, RasGRP3 acts as an important integrating signaling protein of the DAG and Ras signaling pathways and actin polymerization and represents an important therapeutic target in GBM. PMID:25682201

  14. Cell surface GRP78 facilitates hepatoma cells proliferation and migration by activating IGF-IR.

    Science.gov (United States)

    Yin, Yancun; Chen, Chen; Chen, Jinliang; Zhan, Renhui; Zhang, Qiang; Xu, Xiaoyan; Li, Defang; Li, Minjing

    2017-07-01

    The 78kDa glucose regulated protein (GRP78) is a multifunctional chaperone that is involved in a variety of cellular processes. Insulin like growth factor I receptor (IGF-IR) often aberrant expresses in many types of tumor cells. The IGF-IR signaling plays key roles in carcinogenesis and maintenance of the malignant phenotype. The crosstalk between GRP78 and IGF-IR molecules has not well been illuminated. Here, we demonstrated a reciprocal regulation of GRP78 expression and IGF-IR pathway activation. IGF-I induced GRP78 expression in hepatoma cells. IGF-IR knockdown or IGF-IR inhibitor repressed GRP78 expression. Both phosphatidylinositol 3-kianase (PI3K) and mitogen-activated protein kinase (MAPK) pathways involved in IGF-I induction of GRP78 expression. Interestingly, treatment of hepatoma cells with IGF-I re-distributes GRP78 from endoplasmic reticulum (ER) to cell surface and promotes its physical interaction with IGF-IR. Also, GRP78 promotes IGF-IR phosphorylation and activation. Blocked of GRP78 by small interfering RNA or inhibition of GRP78 function by (-)-epigallocatechin gallate (EGCG) blocks IGF-I induced IGF-IR phosphorylation and its downstream signaling. Further, blocked cell surface GRP78 with antibody inhibits IGF-I stimulated cellular proliferation and migration. These data reveal an essential role for the molecular chaperone GRP78 in IGF-IR signaling and implicate the use of GRP78 inhibitors in blocking IGF-IR signaling in hepatoma cells. Copyright © 2017 Elsevier Inc. All rights reserved.

  15. RasGRP1, but not RasGRP3, is required for efficient thymic β-selection and ERK activation downstream of CXCR4.

    Directory of Open Access Journals (Sweden)

    Dominic P Golec

    Full Text Available T cell development is a highly dynamic process that is driven by interactions between developing thymocytes and the thymic microenvironment. Upon entering the thymus, the earliest thymic progenitors, called CD4(-CD8(- 'double negative' (DN thymocytes, pass through a checkpoint termed "β-selection" before maturing into CD4(+CD8(+ 'double positive' (DP thymocytes. β-selection is an important developmental checkpoint during thymopoiesis where developing DN thymocytes that successfully express the pre-T cell receptor (TCR undergo extensive proliferation and differentiation towards the DP stage. Signals transduced through the pre-TCR, chemokine receptor CXCR4 and Notch are thought to drive β-selection. Additionally, it has long been known that ERK is activated during β-selection; however the pathways regulating ERK activation remain unknown. Here, we performed a detailed analysis of the β-selection events in mice lacking RasGRP1, RasGRP3 and RasGRP1 and 3. We report that RasGRP1 KO and RasGRP1/3 DKO deficient thymi show a partial developmental block at the early DN3 stage of development. Furthermore, DN3 thymocytes from RasGRP1 and RasGRP1/3 double knock-out thymi show significantly reduced proliferation, despite expression of the TCRβ chain. As a result of impaired β-selection, the pool of TCRβ(+ DN4 is significantly diminished, resulting in inefficient DN to DP development. Also, we report that RasGRP1 is required for ERK activation downstream of CXCR4 signaling, which we hypothesize represents a potential mechanism of RasGRP1 regulation of β-selection. Our results demonstrate that RasGRP1 is an important regulator of proliferation and differentiation at the β-selection checkpoint and functions downstream of CXCR4 to activate the Ras/MAPK pathway.

  16. High Level Soluble Expression and ATPase Characterization of Human Heat Shock Protein GRP78.

    Science.gov (United States)

    Wu, Shuang; Zhang, Hongpeng; Luo, Miao; Chen, Ke; Yang, Wei; Bai, Lei; Huang, Ailong; Wang, Deqiang

    2017-02-01

    Human GRP78 has been shown to promote cancer progression and is regarded as a novel target for anticancer drugs. However, generation of recombinant full-length GRP78 remains challenging. This report demonstrates that E. coli autoinduction is an excellent method for the preparation of active recombinant GRP78 protein. The final yield was approximately 50 mg/liter of autoinduction culture. Gel-filtration experiments confirmed that the chaperone is a monomer. The purified human GRP78 catalyzed the conversion of ATP to ADP without requiring metal ions as cofactors. Three mutants, T38A, T229A, and S300A, exhibited much lower activity than wild-type GRP78, indicating that the active sites of the ATPase are located at the negatively charged cavity. Three mutants in the negatively charged cavity region dramatically reduced GRP78 activity, further confirming the region as the site of ATPase activity.

  17. Overexpression of molecular chaperons GRP78 and GRP94 in CD44(hi)/CD24(lo) breast cancer stem cells.

    Science.gov (United States)

    Nami, Babak; Ghasemi-Dizgah, Armin; Vaseghi, Akbar

    2016-01-01

    Breast cancer stem cell with CD44(hi)/CD24(lo) phonotype is described having stem cell properties and represented as the main driving factor in breast cancer initiation, growth, metastasis and low response to anti-cancer agents. Glucoseregulated proteins (GRPs) are heat shock protein family chaperons that are charged with regulation of protein machinery and modulation of endoplasmic reticulum homeostasis whose important roles in stem cell development and invasion of various cancers have been demonstrated. Here, we investigated the expression levels of GRP78 and GRP94 in CD44(hi)/CD24(lo) phenotype breast cancer stem cells (BCSCs). MCF7, T-47D and MDA-MB-231 breast cancer cell lines were used. CD44(hi)/CD24(lo) phenotype cell population were analyzed and sorted by fluorescence-activated cell sorting (FACS). Transcriptional and translational expression of GRP78 and GRP94 were investigated by western blotting and quantitative real time PCR. RESULTS showed different proportion of CD44(hi)/CD24(lo) phenotype cell population in their original bulk cells. The ranking of the cell lines in terms of CD44(hi)/CD24(lo) phenotype cell population was as MCF7hi)/CD24(lo) phenotype cells exhibited higher mRNA and protein expression level of GRP78 and GRP94 compared to their original bulk cells. Our results show a relationship between overexpression of GRP78 and GRP94 and exhibiting CD44hi/CD24lo phenotype in breast cancer cells. We conclude that upregulation of GRPs may be an important factor in the emergence of CD44hi/CD24lo phenotype BCSCs features.

  18. Gla-Rich Protein Is a Potential New Vitamin K Target in Cancer: Evidences for a Direct GRP-Mineral Interaction

    Directory of Open Access Journals (Sweden)

    Carla S. B. Viegas

    2014-01-01

    Full Text Available Gla-rich protein (GRP was described in sturgeon as a new vitamin-K-dependent protein (VKDP with a high density of Gla residues and associated with ectopic calcifications in humans. Although VKDPs function has been related with γ-carboxylation, the Gla status of GRP in humans is still unknown. Here, we investigated the expression of recently identified GRP spliced transcripts, the γ-carboxylation status, and its association with ectopic calcifications, in skin basal cell and breast carcinomas. GRP-F1 was identified as the predominant splice variant expressed in healthy and cancer tissues. Patterns of γ-carboxylated GRP (cGRP/undercarboxylated GRP (ucGRP accumulation in healthy and cancer tissues were determined by immunohistochemistry, using newly developed conformation-specific antibodies. Both GRP protein forms were found colocalized in healthy tissues, while ucGRP was the predominant form associated with tumor cells. Both cGRP and ucGRP found at sites of microcalcifications were shown to have in vitro calcium mineral-binding capacity. The decreased levels of cGRP and predominance of ucGRP in tumor cells suggest that GRP may represent a new target for the anticancer potential of vitamin K. Also, the direct interaction of cGRP and ucGRP with BCP crystals provides a possible mechanism explaining GRP association with pathological mineralization.

  19. Receptors for GRP/bombesin-like peptides in the rat forebrain

    International Nuclear Information System (INIS)

    Wolf, S.S.; Moody, T.W.

    1985-01-01

    Binding sites in the rat forebrain were characterized using ( 125 I-Tyr4)bombesin as a receptor probe. Pharmacology experiments indicate that gastrin releasing peptide (GRP) and the GRP fragments GRP as well as Ac-GRP inhibited radiolabeled (Tyr4)bombesin binding with high affinity. Biochemistry experiments indicated that heat, N-ethyl maleimide or trypsin greatly reduced radiolabeled (Tyr4)bombesin binding. Also, autoradiographic studies indicated that highest grain densities were present in the stria terminalis, periventricular and suprachiasmatic nucleus of the hypothalamus, dorsomedial and rhomboid thalamus, dentate gyrus, hippocampus and medial amygdaloid nucleus. The data suggest that CNS protein receptors, which are discretely distributed in the rat forebrain, may mediate the action of endogenous GRP/bombesin-like peptides

  20. Suppress flashover of GRP fire with water mist inside ISO 9705 Room

    Directory of Open Access Journals (Sweden)

    Qiang Xu

    2011-01-01

    Full Text Available Water mist suppression tests for glass-reinforced polyester (GRP panels were conducted in ISO 9705 room. GRP panels covered part of the room and a wood crib fire was used as fire source to ignite GRP fire. A four-nozzle water mist suppression equipment was used inside test room on the time of flashover. Heat release rate of the combustion inside the room, room temperature, surface temperature of GRP panels, total heat flux to wall, ceiling and floor in specific positions were measured. Gas concentration of O2, CO, and CO2 was also measured in the corner of the room at two different levels. A thermal image video was used to record the suppression procedure inside room. Test results show that the water mist system is efficient in suppressing the flashover of GRP fire and cooling the room within short time.

  1. Second Generation Grp94-Selective Inhibitors Provide Opportunities for the Inhibition of Metastatic Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Crowley, Vincent M. [Department of Medicinal Chemistry, The University of Kansas, 1251 Wescoe Hall Dr. Malott 4070 Lawrence KS 66045 USA; Huard, Dustin J. E. [School of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta GA 30332 USA; Lieberman, Raquel L. [School of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta GA 30332 USA; Blagg, Brian S. J. [Warren Family Research Center for Drug Discovery and Development, and Department of Chemistry & Biochemistry, University of Notre Dame, 305 McCourtney Hall Notre Dame IN 46556 USA

    2017-09-27

    Glucose regulated protein 94 (Grp94) is the endoplasmic reticulum (ER) resident isoform of the 90 kDa heat shock protein (Hsp90) family and its inhibition represents a promising therapeutic target for the treatment of many diseases. Modification of the first generation cis-amide bioisostere imidazole to alter the angle between the resorcinol ring and the benzyl side chain via cis-amide replacements produced compounds with improved Grp94 affinity and selectivity. Structure–activity relationship studies led to the discovery of compound 30, which exhibits 540 nm affinity and 73-fold selectivity towards Grp94. Grp94 is responsible for the maturation and trafficking of proteins associated with cell signaling and motility, including select integrins. The Grp94-selective inhibitor 30 was shown to exhibit potent anti-migratory effects against multiple aggressive and metastatic cancers.

  2. Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP in the unanesthetized trout

    Directory of Open Access Journals (Sweden)

    Jean-Claude Le Mével

    2013-07-01

    Gastrin-releasing peptide (GRP, a neuropeptide initially isolated from porcine stomach, shares sequence similarity with bombesin. GRP and its receptors are present in the brains and peripheral tissues of several species of teleost fish, but little is known about the ventilatory and cardiovascular effects of this peptide in these vertebrates. The goal of this study was to compare the central and peripheral actions of picomolar doses of trout GRP on ventilatory and cardiovascular variables in the unanesthetized rainbow trout. Compared to vehicle, intracerebroventricular (ICV injection of GRP (1–50 pmol significantly elevated the ventilation rate (ƒV and the ventilation amplitude (VAMP, and consequently the total ventilation (VTOT. The maximum hyperventilatory effect of GRP (VTOT: +225%, observed at a dose of 50 pmol, was mostly due to its stimulatory action on VAMP (+170% rather than ƒV (+20%. In addition, ICV GRP (50 pmol produced a significant increase in mean dorsal aortic blood pressure (PDA (+35% and in heart rate (ƒH (+25%. Intra-arterial injections of GRP (5–100 pmol were without sustained effect on the ventilatory variables but produced sporadic and transient increases in ventilatory movement at doses of 50 and 100 pmol. At these doses, GRP elevated PDA by +20% but only the 50 pmol dose significantly increased HR (+15%. In conclusion, our study suggests that endogenous GRP within the brain of the trout may act as a potent neurotransmitter and/or neuromodulator in the regulation of cardio-ventilatory functions. In the periphery, endogenous GRP may act as locally-acting and/or circulating neurohormone with an involvement in vasoregulatory mechanisms.

  3. Isthmin targets cell-surface GRP78 and triggers apoptosis via induction of mitochondrial dysfunction

    Science.gov (United States)

    Chen, M; Zhang, Y; Yu, V C; Chong, Y-S; Yoshioka, T; Ge, R

    2014-01-01

    Isthmin (ISM) is a secreted 60-kDa protein that potently induces endothelial cell (EC) apoptosis. It suppresses tumor growth and angiogenesis in mice when stably overexpressed in cancer cells. Although αvβ5 integrin serves as a low-affinity receptor for ISM, the mechanism by which ISM mediates antiangiogenesis and apoptosis in ECs remain to be fully resolved. In this work, we report the identification of cell-surface glucose-regulated protein 78 kDa (GRP78) as a high-affinity receptor for ISM (Kd=8.6 nM). We demonstrated that ISM-GRP78 interaction triggers apoptosis not only in activated ECs but also in cancer cells expressing high level of cell-surface GRP78. Normal cells and benign tumor cells tend to express low level of cell-surface GRP78 and are resistant to ISM-induced apoptosis. Upon binding to GRP78, ISM is internalized into ECs through clathrin-dependent endocytosis that is essential for its proapoptotic activity. Once inside the cell, ISM co-targets with GRP78 to mitochondria where it interacts with ADP/ATP carriers on the inner membrane and blocks ATP transport from mitochondria to cytosol, thereby causing apoptosis. Hence, ISM is a novel proapoptotic ligand that targets cell-surface GRP78 to trigger apoptosis by inducing mitochondrial dysfunction. The restricted and high-level expression of cell-surface GRP78 on cancer cells and cancer ECs make them uniquely susceptible to ISM-targeted apoptosis. Indeed, systemic delivery of recombinant ISM potently suppressed subcutaneous 4T1 breast carcinoma and B16 melanoma growth in mice by eliciting apoptosis selectively in the cancer cells and cancer ECs. Together, this work reveals a novel ISM-GRP78 apoptosis pathway and demonstrates the potential of ISM as a cancer-specific and dual-targeting anticancer agent. PMID:24464222

  4. Isthmin targets cell-surface GRP78 and triggers apoptosis via induction of mitochondrial dysfunction.

    Science.gov (United States)

    Chen, M; Zhang, Y; Yu, V C; Chong, Y-S; Yoshioka, T; Ge, R

    2014-05-01

    Isthmin (ISM) is a secreted 60-kDa protein that potently induces endothelial cell (EC) apoptosis. It suppresses tumor growth and angiogenesis in mice when stably overexpressed in cancer cells. Although αvβ5 integrin serves as a low-affinity receptor for ISM, the mechanism by which ISM mediates antiangiogenesis and apoptosis in ECs remain to be fully resolved. In this work, we report the identification of cell-surface glucose-regulated protein 78 kDa (GRP78) as a high-affinity receptor for ISM (Kd=8.6 nM). We demonstrated that ISM-GRP78 interaction triggers apoptosis not only in activated ECs but also in cancer cells expressing high level of cell-surface GRP78. Normal cells and benign tumor cells tend to express low level of cell-surface GRP78 and are resistant to ISM-induced apoptosis. Upon binding to GRP78, ISM is internalized into ECs through clathrin-dependent endocytosis that is essential for its proapoptotic activity. Once inside the cell, ISM co-targets with GRP78 to mitochondria where it interacts with ADP/ATP carriers on the inner membrane and blocks ATP transport from mitochondria to cytosol, thereby causing apoptosis. Hence, ISM is a novel proapoptotic ligand that targets cell-surface GRP78 to trigger apoptosis by inducing mitochondrial dysfunction. The restricted and high-level expression of cell-surface GRP78 on cancer cells and cancer ECs make them uniquely susceptible to ISM-targeted apoptosis. Indeed, systemic delivery of recombinant ISM potently suppressed subcutaneous 4T1 breast carcinoma and B16 melanoma growth in mice by eliciting apoptosis selectively in the cancer cells and cancer ECs. Together, this work reveals a novel ISM-GRP78 apoptosis pathway and demonstrates the potential of ISM as a cancer-specific and dual-targeting anticancer agent.

  5. RasGRP1 confers the phorbol ester-sensitive phenotype to EL4 lymphoma cells.

    Science.gov (United States)

    Han, Shujie; Knoepp, Stewart M; Hallman, Mark A; Meier, Kathryn E

    2007-01-01

    The murine EL4 lymphoma cell line exists in variants that are either sensitive or resistant to the tumor promoter phorbol 12-myristate 13-acetate (PMA). In sensitive EL4 cells, PMA causes robust Erk mitogen-activated protein kinase activation that results in growth arrest. In resistant cells, PMA induces minimal Erk activation, without growth arrest. PMA stimulates IL-2 production in sensitive, but not resistant, cells. The role of RasGRP1, a PMA-activated guanine nucleotide exchange factor for Ras, in EL4 phenotype was examined. Endogenous RasGRP1 protein is expressed at much higher levels in sensitive than in resistant cells. PMA-induced Ras activation is observed in sensitive cells but not in resistant cells lacking Ras-GRP1. PMA induces down-regulation of RasGRP1 protein in sensitive cells but increases RasGRP1 in resistant cells. Transfection of RasGRP1 into resistant cells enhances PMA-induced Erk activation. In the reverse experiment, introduction of small interfering RNA (siRNA) for RasGRP1 suppresses PMA-induced Ras and Erk activations in sensitive cells. Sensitive cells incubated with siRNA for RasGRP1 exhibit the PMA-resistant phenotype, in that they are able to proliferate in the presence of PMA and do not secrete IL-2 when stimulated with PMA. These studies indicate that the PMA-sensitive phenotype, as previously defined for the EL4 cell line, is conferred by endogenous expression of RasGRP1 protein.

  6. Seleção de progênies de eucalipto pelo índice Z por MQM e Blup

    Directory of Open Access Journals (Sweden)

    Cristiane Aparecida Fioravante Reis

    2011-05-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência do índice Z (índice somatório das variáveis padronizadas na seleção de progênies de irmãos completos entre Eucalyptus grandis e Eucalyptus urophylla via método dos quadrados mínimos (MQM e da melhor predição linear não viesada (Blup. Os experimentos foram realizados em dois tipos de solo e de ambiente, em blocos ao acaso com cinco repetições, oito plantas por parcela e espaçamento de 3x2 m. Aos três anos de idade, as plantas foram avaliadas quanto ao incremento médio anual de madeira, à densidade básica, ao rendimento de celulose e ao álcali efetivo. Para a comparação da eficiência da seleção das melhores progênies, pelos procedimentos MQM e Blup, foi obtido o índice Z para cada indivíduo. Posteriormente, foram obtidas as médias preditas das progênies para o índice Z, ranqueadas em sentido favorável ao melhoramento. O índice clássico também foi utilizado. A coincidência entre as melhores progênies selecionadas pelos índices clássico e Z foi boa. No entanto, o índice Z permite visualização gráfica, o que possibilita verificar em quais caracteres uma determinada progênie apresenta alguma deficiência. Não há diferença entre as melhores progênies selecionadas pelo índice Z via MQM e Blup.

  7. Secretion of the endoplasmic reticulum stress protein, GRP78, into the BALF is increased in cigarette smokers.

    Science.gov (United States)

    Aksoy, Mark O; Kim, Victor; Cornwell, William D; Rogers, Thomas J; Kosmider, Beata; Bahmed, Karim; Barrero, Carlos; Merali, Salim; Shetty, Neena; Kelsen, Steven G

    2017-05-02

    Identification of biomarkers of cigarette smoke -induced lung damage and early COPD is an area of intense interest. Glucose regulated protein of 78 kD (i.e., GRP78), a multi-functional protein which mediates cell responses to oxidant stress, is increased in the lungs of cigarette smokers and in the serum of subjects with COPD. We have suggested that secretion of GRP78 by lung cells may explain the increase in serum GRP78 in COPD. To assess GRP78 secretion by the lung, we assayed GRP78 in bronchoalveolar lavage fluid (BALF) in chronic smokers and non-smokers. We also directly assessed the acute effect of cigarette smoke material on GRP78 secretion in isolated human airway epithelial cells (HAEC). GRP78 was measured in BALF of smokers (S; n = 13) and non-smokers (NS; n = 11) by Western blotting. GRP78 secretion by HAEC was assessed by comparing its concentration in cell culture medium and cell lysates. Cells were treated for 24 h with either the volatile phase of cigarette smoke (cigarette smoke extract (CSE) or the particulate phase (cigarette smoke condensate (CSC)). GRP78 was present in the BALF of both NS and S but levels were significantly greater in S (p = 0.04). GRP78 was secreted constitutively in HAEC. CSE 15% X 24 h increased GRP78 in cell-conditioned medium without affecting its intracellular concentration. In contrast, CSC X 24 h increased intracellular GRP78 expression but did not affect GRP78 secretion. Brefeldin A, an inhibitor of classical Golgi secretion pathways, did not inhibit GRP78 secretion indicating that non-classical pathways were involved. The present study indicates that GRP78 is increased in BALF in cigarette smokers; that HAEC secrete GRP78; and that GRP78 secretion by HAEC is augmented by cigarette smoke particulates. Enhanced secretion of GRP78 by lung cells makes it a potential biomarker of cigarette smoke-induced lung injury.

  8. Semi-automatic handling of meteorological ground measurements using WeatherProg: prospects and practical implications

    Science.gov (United States)

    Langella, Giuliano; Basile, Angelo; Bonfante, Antonello; De Mascellis, Roberto; Manna, Piero; Terribile, Fabio

    2016-04-01

    WeatherProg is a computer program for the semi-automatic handling of data measured at ground stations within a climatic network. The program performs a set of tasks ranging from gathering raw point-based sensors measurements to the production of digital climatic maps. Originally the program was developed as the baseline asynchronous engine for the weather records management within the SOILCONSWEB Project (LIFE08 ENV/IT/000408), in which daily and hourly data where used to run water balance in the soil-plant-atmosphere continuum or pest simulation models. WeatherProg can be configured to automatically perform the following main operations: 1) data retrieval; 2) data decoding and ingestion into a database (e.g. SQL based); 3) data checking to recognize missing and anomalous values (using a set of differently combined checks including logical, climatological, spatial, temporal and persistence checks); 4) infilling of data flagged as missing or anomalous (deterministic or statistical methods); 5) spatial interpolation based on alternative/comparative methods such as inverse distance weighting, iterative regression kriging, and a weighted least squares regression (based on physiography), using an approach similar to PRISM. 6) data ingestion into a geodatabase (e.g. PostgreSQL+PostGIS or rasdaman). There is an increasing demand for digital climatic maps both for research and development (there is a gap between the major of scientific modelling approaches that requires digital climate maps and the gauged measurements) and for practical applications (e.g. the need to improve the management of weather records which in turn raises the support provided to farmers). The demand is particularly burdensome considering the requirement to handle climatic data at the daily (e.g. in the soil hydrological modelling) or even at the hourly time step (e.g. risk modelling in phytopathology). The key advantage of WeatherProg is the ability to perform all the required operations and

  9. BAG3 sensitizes cancer cells exposed to DNA damaging agents via direct interaction with GRP78.

    Science.gov (United States)

    Kong, De-Hui; Zhang, Qiang; Meng, Xin; Zong, Zhi-Hong; Li, Chao; Liu, Bao-Qin; Guan, Yifu; Wang, Hua-Qin

    2013-12-01

    Bcl-2 associated athanogene 3 (BAG3) has a modular structure that contains a BAG domain, a WW domain, a proline-rich (PxxP) domain to mediate potential interactions with chaperons and other proteins that participate in more than one signal transduction. In search for novel interacting partners, the current study identified that 78kDa glucose-regulated protein (GRP78) was a novel partner interacting with BAG3. Interaction between GRP78 and BAG3 was confirmed by coimmunoprecipitation and glutathione S-transferase (GST) pulldown. We also identified that the ATPase domain of GRP78 and BAG domain of BAG3 mediated their interaction. Counterintuitive for a prosurvival protein, BAG3 was found to promote the cytotoxicity of breast cancer MCF7, thyroid cancer FRO and glioma U87 cells subjected to genotoxic stress. In addition, the current study demonstrated that BAG3 interfered with the formation of the antiapoptotic GRP78-procaspase-7 complex, which resulted in an increased genotoxic stress-induced cytotoxicity in cancer cells. Furthermore, overexpression of GRP78 significantly blocked the enhancing effects of BAG3 on activation of caspase-7 and induction of apoptosis by genotoxic stress. Overall, these results suggested that through direct interaction BAG3 could prevent the antiapoptotic effect of GRP78 upon genotoxic stress. © 2013.

  10. Divergência genética entre progênies de pupunheira quanto a caracteres de palmito

    Directory of Open Access Journals (Sweden)

    Jacson Rondinelli da Silva Negreiros

    2013-05-01

    Full Text Available O objetivo deste trabalho foi estimar a divergência genética de progênies de pupunheira, com base em variáveis agronômicas do palmito. O delineamento experimental em blocos ao acaso foi utilizado em látice triplo 10x10, com 100 tratamentos (progênies e três repetições. Foram feitas as seguintes avaliações: massa da base do palmito, produtividade do palmito de primeira e de segunda qualidade, número de plantas por parcela e diâmetro do palmito. A divergência genética foi estimada pela distância generalizada de Mahalanobis, como medida de dissimilaridade, e pelo método de otimização de Tocher e variáveis canônicas, na formação de grupos. As progênies formaram 26 grupos distintos. A variância acumulada pelas três primeiras variáveis canônicas foi de 75,12%. Planta por parcela e massa do palmito de segunda, indiretamente relacionada à massa do palmito de primeira, foram os caracteres mais importantes na discriminação das progênies, na primeira variável canônica (35,77%. A massa da base do palmito apresentou relação indireta com a produtividade do palmito de segunda e plantas por parcela, na segunda variável canônica (22.93%, e com a massa do palmito de primeira na terceira variável canônica (16,42%. Dezessete progênies de quatro grupos divergentes (G3, G5, G11 e G12 têm potencial para seleção quanto à produtividade de palmito no programa de melhoramento da pupunheira.

  11. Randomized Trial of a Group Music and Imagery Method (GrpMI) for Women with Fibromyalgia.

    Science.gov (United States)

    Torres, Esperanza; Pedersen, Inge N; Pérez-Fernández, José I

    2018-06-07

    Fibromyalgia (FM) affects about 2-4% of the world population. Patients, mostly women, experience chronic widespread pain, fatigue, stiffness, sleep disturbances, and psychological disorders, especially depression and anxiety. The aim of this study was to examine preliminary efficacy of a Group Music and Imagery (GrpMI) intervention, which included relaxation, music listening, and spontaneous imagery, to improve subjective psychological well-being, functional capacity and health, pain perception, anxiety, and depression in women with FM. Fifty-six women aged 35 to 65 years (M = 51.3) diagnosed with FM were randomly assigned to either GrpMI treatment (n = 33) or control (n = 26) condition. Experimental group participants received 12 weekly GrpMI sessions, and control group participants who did not receive any additional service completed measures at the same time points as the experimental group. Intra-group analyses showed that GrpMI participants had a significant increase in psychological well-being and significant decrease in the impact of FM on functional capacity and health, pain perception, anxiety, and depression post-treatment, with sustained benefit at three-month follow-up for all variables except psychological well-being. Control group participants showed decreases in trait anxiety and depression at post-treatment, with no significant benefit at three-month follow-up. Inter-group analyses showed that compared with control participants, GRpMI participants had significantly higher scores for psychological well-being and lower-state anxiety post-treatment; however, no differences were observed between groups at three-month follow-up. Findings offer preliminary evidence for the benefit of GrpMI to improve well-being and reduce anxiety in women with FM. Findings also suggest that GrpMI may help diminish pain intensity, state depression, and the impact of FM on functional capacity and health, but further studies are needed to establish efficacy.

  12. Adenosine-derived inhibitors of 78 kDa glucose regulated protein (Grp78) ATPase: insights into isoform selectivity.

    Science.gov (United States)

    Macias, Alba T; Williamson, Douglas S; Allen, Nicola; Borgognoni, Jenifer; Clay, Alexandra; Daniels, Zoe; Dokurno, Pawel; Drysdale, Martin J; Francis, Geraint L; Graham, Christopher J; Howes, Rob; Matassova, Natalia; Murray, James B; Parsons, Rachel; Shaw, Terry; Surgenor, Allan E; Terry, Lindsey; Wang, Yikang; Wood, Mike; Massey, Andrew J

    2011-06-23

    78 kDa glucose-regulated protein (Grp78) is a heat shock protein (HSP) involved in protein folding that plays a role in cancer cell proliferation. Binding of adenosine-derived inhibitors to Grp78 was characterized by surface plasmon resonance and isothermal titration calorimetry. The most potent compounds were 13 (VER-155008) with K(D) = 80 nM and 14 with K(D) = 60 nM. X-ray crystal structures of Grp78 bound to ATP, ADPnP, and adenosine derivative 10 revealed differences in the binding site between Grp78 and homologous proteins.

  13. The Natural History of the Progression of Atrophy Secondary to Stargardt Disease (ProgStar) Studies: Design and Baseline Characteristics: ProgStar Report No. 1.

    Science.gov (United States)

    Strauss, Rupert W; Ho, Alex; Muñoz, Beatriz; Cideciyan, Artur V; Sahel, José-Alain; Sunness, Janet S; Birch, David G; Bernstein, Paul S; Michaelides, Michel; Traboulsi, Elias I; Zrenner, Eberhart; Sadda, SriniVas; Ervin, Ann-Margret; West, Sheila; Scholl, Hendrik P N

    2016-04-01

    To describe the design and baseline characteristics of patients enrolled into 2 natural history studies of Stargardt disease (STGD1). Multicenter retrospective and prospective cohort studies. Three hundred sixty-five unique patients aged 6 years and older at baseline harboring disease-causing variants in the ABCA4 gene and with specified ocular lesions were enrolled from 9 centers in the United States and Europe. In the retrospective study, patients contributed medical record data from at least 2 and up to 4 visits for at least 1 examination modality: fundus autofluorescence (FAF), spectral-domain (SD) optical coherence tomography (SD OCT), and/or microperimetry (MP). The total observational period was at least 2 years and up to 5 years between single visits. Demographic and visual acuity (VA) data also were obtained. In the prospective study, eligible patients were examined at baseline using a standard protocol, with 6-month follow-up visits planned for a 2-year period for serial Early Treatment Diabetic Retinopathy Study (ETDRS) best-corrected VA, SD OCT, FAF, and MP. Design and rationale of a multicenter study to determine the progression of STGD1 in 2 large retrospective and prospective international cohorts. Detailed baseline characteristics of both cohorts are presented, including demographics, and structural and functional retinal metrics. Into the retrospective study, 251 patients (458 eyes) were enrolled; mean follow-up ± standard deviation was 3.9±1.6 years. At baseline, 36% had no or mild VA loss, and 47% of the study eyes had areas of definitely decreased autofluorescence (DDAF) with an average lesion area of 2.5±2.9 mm(2) (range, 0.02-16.03 mm(2)). Two hundred fifty-nine patients (489 eyes) were enrolled in the prospective study. At baseline, 20% had no or mild VA loss, and 64% had areas of DDAF with an average lesion area of 4.0±4.4 mm(2) (range, 0.03-24.24 mm(2)). The mean retinal sensitivity with MP was 10.8±5.0 dB. The ProgStar cohorts have

  14. Variação sazonal da fotossíntese e clorofila em progênies de Grevillea robusta Cunn

    OpenAIRE

    Inoue, Mario Takao; UNICENTRO; Martins, Emerson Gonçalves; EMBRAPA

    2009-01-01

    Avaliou-se a fotossíntese e o teor de clorofila em seis progênies de Grevillea robusta Cunn. durante um ano, visando detectar possíveis variações sazonais. As progênies demonstraram reações distintas entre si e entre as estações do ano, sugerindo que as grandezas fisiológicas podem servir como indicadores do comportamento genético aos quatro anos de idade das progênies testadas. A fotossíntese elevou-se no sentido do inverno para primavera e verão, para decair no sentido do outono-inverno. A ...

  15. Inhibition of GRP78 abrogates radioresistance in oropharyngeal carcinoma cells after EGFR inhibition by cetuximab.

    Directory of Open Access Journals (Sweden)

    Chaonan Sun

    Full Text Available The EGFR-specific mAb cetuximab is one of the most effective treatments for oropharyngeal carcinoma, while patient responses to EGFR inhibitors given alone are modest. Combination treatment with radiation can improve the efficacy of treatment through increasing radiosensitivity, while resistance to radiation after administration of cetuximab limits its efficiency. Radiation and drugs can damage the endoplasmic reticulum (ER homeostatic state and result in ER stress (ERS, subsequently causing resistance to radiation and drugs. Whether the ERS pathway is involved in radioresistance after administration of cetuximab has not been reported. Herein, we show that cetuximab could increase the radiosensitivity of FaDu cells but not Detroit562 cells. In addition, cetuximab inhibited the radiation-induced activation of the ERS signalling pathway IRE1α/ATF6-GRP78 in FaDu cells, while this effect was absent in Detroit562 cells. Silencing GRP78 increased the radiosensitivity of oropharyngeal carcinoma cells and inhibited radiation-induced DNA double-strand-break (DSB repair and autophagy. More interestingly, silencing GRP78 abrogated resistance to cetuximab and radiation in Detroit562 cells and had a synergistic effect with cetuximab in increasing the radiosensitivity of FaDu cells. Immunohistochemistry showed that overexpression of both GRP78 and EGFR was associated with a poor prognosis in oropharyngeal carcinoma patients (P<0.05. Overall, the results of this study show that radioresistance after EGFR inhibition by cetuximab is mediated by the ERS signalling pathway IRE1α/ATF6-GRP78. This suppression was consequently unable to inhibit radiation-induced DSB repair and autophagy in oropharyngeal carcinoma cells, which conferred resistance to radiotherapy and cetuximab. These results suggest that the cooperative effects of radiotherapy and cetuximab could be further improved by inhibiting GRP78 in non-responsive oropharyngeal carcinoma patients.

  16. Cell surface localization of the 78 kD glucose regulated protein (GRP 78) induced by thapsigargin.

    Science.gov (United States)

    Delpino, A; Piselli, P; Vismara, D; Vendetti, S; Colizzi, V

    1998-01-01

    In the present study it was found that the synthesis of the 78 kD glucose-regulated protein (GRP 78 or BIP) is vigorously induced in human rabdomiosarcoma cells (TE 671/RD) following both short-term (1 h) and prolonged (18 h) exposure to 100 nM thapsigargin (Tg). Flow cytometric analysis with a specific anti-GRP 78 polyclonal antibody showed that Tg-treated cells express the GRP 78 on the plasma membrane. Cell surface localization of the Tg-induced GRP 78 was confirmed by biotinylation of membrane-exposed proteins and subsequent isolation of the biotin-labelled proteins by streptavidin/agarose affinity chromatography. It was found that a fraction of the Tg-induced GRP 78 is present among the biotin-labelled, surface-exposed, proteins. Conversely, the GRP 78 immunoprecipitated from unfractionated lysates of Tg-treated and biotin-reacted cells was found to be biotinylated. This is the first report demonstrating surface expression of GRP 78 in cells exposed to a specific GRP 78-inducing stimulus.

  17. Optimized Chemical Probes for REV-ERBα

    OpenAIRE

    Trump, Ryan P.; Bresciani, Stefano; Cooper, Anthony W. J.; Tellam, James P.; Wojno, Justyna; Blaikley, John; Orband-Miller, Lisa A.; Kashatus, Jennifer A.; Dawson, Helen C.; Loudon, Andrew; Ray, David; Grant, Daniel; Farrow, Stuart N.; Willson, Timothy M.; Tomkinson, Nicholas C. O.

    2013-01-01

    REV-ERBα has emerged as an important target for regulation of circadian rhythm and its associated physiology. Herein, we report on the optimization of a series of REV-ERBα agonists based on GSK4112 (1) for potency, selectivity, and bioavailability. Potent REV-ERBα agonists 4, 10, 16, and 23 are detailed for their ability to suppress BMAL and IL-6 expression from human cells while also demonstrating excellent selectivity over LXRα. Amine 4 demonstrated in vivo bioavailability after either IV o...

  18. Eficiência de métodos de melhoramento para teor de fibra e produtividade de grãos em progênies de feijoeiro comum

    Directory of Open Access Journals (Sweden)

    Adélia Cristina Fernandes Silva

    2013-01-01

    Full Text Available O objetivo deste estudo foi comparar métodos de melhoramento quanto à variabilidade genética e à eficiência na obtenção de progênies superiores para teor de fibra bruta e produtividade de grãos em feijoeiro comum. As progênies foram obtidas do cruzamento, em 2003, entre as linhagens carioca CNFC 7812 e CNFC 7829. A população segregante foi conduzida por bulk, bulk dentro de F2 e SSD até geração F7. Em 2008 foram amostradas aleatoriamente 64 progênies de cada método para avaliação em Goiás e Paraná. Foram obtidas as estimativas dos componentes de variância, parâmetros genéticos e fenotípicos, média e índices de geração de progênies superiores. As progênies obtidas pelos métodos de bulk e SSD apresentaram diferenças significativas para produtividade e teor de fibra bruta e maior variância genética. O bulk dentro de F2 apresentou os maiores índices de geração de progênies superiores e maiores médias para os dois caracteres, sendo recomendado para os programas de melhoramento de feijoeiro comum.

  19. GRP94 Regulates Circulating Cholesterol Levels through Blockade of PCSK9-Induced LDLR Degradation

    Directory of Open Access Journals (Sweden)

    Steve Poirier

    2015-12-01

    Full Text Available Clearance of circulating low-density lipoprotein cholesterol (LDLc by hepatic LDL receptors (LDLR is central for vascular health. Secreted by hepatocytes, PCSK9 induces the degradation of LDLR, resulting in higher plasma LDLc levels. Still, it remains unknown why LDLR and PCSK9 co-exist within the secretory pathway of hepatocytes without leading to complete degradation of LDLR. Herein, we identified the ER-resident GRP94, and more precisely its client-binding C-terminal domain, as a PCSK9-LDLR inhibitory binding protein. Depletion of GRP94 did not affect calcium homeostasis, induce ER stress, nor did it alter PCSK9 processing or its secretion but greatly increased its capacity to induce LDLR degradation. Accordingly, we found that hepatocyte-specific Grp94-deficient mice have higher plasma LDLc levels correlated with ∼80% reduction in hepatic LDLR protein levels. Thus, we provide evidence that, in physiological conditions, binding of PCSK9 to GRP94 protects LDLR from degradation likely by preventing early binding of PCSK9 to LDLR within the ER.

  20. Which way is the wind blowing for GRP? Materials and processes for manufacturing rotor blades

    Energy Technology Data Exchange (ETDEWEB)

    Bittmann, E. [Werkstoff and Struktur, Herreth (Germany)

    2002-11-01

    The material technology for the production of large-area rotor blades made of glass reinforced plastics (GRP) constitutes an important milestone in development. Customised resin systems, flexible, automated processing methods and high component quality document the progress made in the wind power industry. (orig.)

  1. Targeting HSP70 and GRP78 in canine osteosarcoma cells in combination with doxorubicin chemotherapy.

    Science.gov (United States)

    Asling, Jonathan; Morrison, Jodi; Mutsaers, Anthony J

    2016-11-01

    Heat shock proteins (HSPs) are molecular chaperones subdivided into several families based on their molecular weight. Due to their cytoprotective roles, these proteins may help protect cancer cells against chemotherapy-induced cell death. Investigation into the biologic activity of HSPs in a variety of cancers including primary bone tumors, such as osteosarcoma (OSA), is of great interest. Both human and canine OSA tumor samples have aberrant production of HSP70. This study assessed the response of canine OSA cells to inhibition of HSP70 and GRP78 by the ATP-mimetic VER-155008 and whether this treatment strategy could sensitize cells to doxorubicin chemotherapy. Single-agent VER-155008 treatment decreased cellular viability and clonogenic survival and increased apoptosis in canine OSA cell lines. However, combination schedules with doxorubicin after pretreatment with VER-155008 did not improve inhibition of cellular viability, apoptosis, or clonogenic survival. Treatment with VER-155008 prior to chemotherapy resulted in an upregulation of target proteins HSP70 and GRP78 in addition to the co-chaperone proteins Herp, C/EBP homologous transcription protein (CHOP), and BAG-1. The increased GRP78 was more cytoplasmic in location compared to untreated cells. Single-agent treatment also revealed a dose-dependent reduction in activated and total Akt. Based on these results, targeting GRP78 and HSP70 may have biologic activity in canine osteosarcoma. Further studies are required to determine if and how this strategy may impact the response of osteosarcoma cells to chemotherapy.

  2. The endothelial cell receptor GRP78 is required for mucormycosis pathogenesis in diabetic mice

    Science.gov (United States)

    Liu, Mingfu; Spellberg, Brad; Phan, Quynh T.; Fu, Yue; Fu, Yong; Lee, Amy S.; Edwards, John E.; Filler, Scott G.; Ibrahim, Ashraf S.

    2010-01-01

    Mucormycosis is a fungal infection of the sinuses, brain, or lungs that causes a mortality rate of at least 50% despite first-line therapy. Because angioinvasion is a hallmark of mucormycosis infections, we sought to define the endothelial cell receptor(s) for fungi of the order Mucorales (the fungi that cause mucormycosis). Furthermore, since patients with elevated available serum iron, including those with diabetic ketoacidosis (DKA), are uniquely susceptible to mucormycosis, we sought to define the role of iron and glucose in regulating the expression of such a receptor. Here, we have identified glucose-regulated protein 78 (GRP78) as what we believe to be a novel host receptor that mediates invasion and damage of human endothelial cells by Rhizopus oryzae, the most common etiologic species of Mucorales, but not Candida albicans or Aspergillus fumigatus. Elevated concentrations of glucose and iron, consistent with those seen during DKA, enhanced GRP78 expression and the resulting R. oryzae invasion and damage of endothelial cells in a receptor-dependent manner. Mice with DKA, which have enhanced susceptibility to mucormycosis, exhibited increased expression of GRP78 in sinus, lungs, and brain compared with normal mice. Finally, GRP78-specific immune serum protected mice with DKA from mucormycosis. These results suggest a unique susceptibility of patients with DKA to mucormycosis and provide a foundation for the development of new therapeutic interventions for these deadly infections. PMID:20484814

  3. Targeting GRP75 improves HSP90 inhibitor efficacy by enhancing p53-mediated apoptosis in hepatocellular carcinoma.

    Directory of Open Access Journals (Sweden)

    Weiwei Guo

    Full Text Available Heat shock protein 90 (HSP90 inhibitors are potential drugs for cancer therapy. The inhibition of HSP90 on cancer cell growth largely through degrading client proteins, like Akt and p53, therefore, triggering cancer cell apoptosis. Here, we show that the HSP90 inhibitor 17-AAG can induce the expression of GRP75, a member of heat shock protein 70 (HSP70 family, which, in turn, attenuates the anti-growth effect of HSP90 inhibition on cancer cells. Additionally, 17-AAG enhanced binding of GRP75 and p53, resulting in the retention of p53 in the cytoplasm. Blocking GRP75 with its inhibitor MKT-077 potentiated the anti-tumor effects of 17-AAG by disrupting the formation of GRP75-p53 complexes, thereby facilitating translocation of p53 into the nuclei and leading to the induction of apoptosis-related genes. Finally, dual inhibition of HSP90 and GRP75 was found to significantly inhibit tumor growth in a liver cancer xenograft model. In conclusion, the GRP75 inhibitor MKT-077 enhances 17-AAG-induced apoptosis in HCCs and increases p53-mediated inhibition of tumor growth in vivo. Dual targeting of GRP75 and HSP90 may be a useful strategy for the treatment of HCCs.

  4. Hypoxic Preconditioning Promotes the Bioactivities of Mesenchymal Stem Cells via the HIF-1α-GRP78-Akt Axis.

    Science.gov (United States)

    Lee, Jun Hee; Yoon, Yeo Min; Lee, Sang Hun

    2017-06-21

    Mesenchymal stem cells (MSC) are ideal materials for stem cell-based therapy. As MSCs reside in hypoxic microenvironments (low oxygen tension of 1% to 7%), several studies have focused on the beneficial effects of hypoxic preconditioning on MSC survival; however, the mechanisms underlying such effects remain unclear. This study aimed to uncover the potential mechanism involving 78-kDa glucose-regulated protein (GRP78) to explain the enhanced MSC bioactivity and survival in hindlimb ischemia. Under hypoxia (2% O₂), the expression of GRP78 was significantly increased via hypoxia-inducible factor (HIF)-1α. Hypoxia-induced GRP78 promoted the proliferation and migration potential of MSCs through the HIF-1α-GRP78-Akt signal axis. In a murine hind-limb ischemia model, hypoxic preconditioning enhanced the survival and proliferation of transplanted MSCs through suppression of the cell death signal pathway and augmentation of angiogenic cytokine secretion. These effects were regulated by GRP78. Our findings indicate that hypoxic preconditioning promotes survival, proliferation, and angiogenic cytokine secretion of MSCs via the HIF-1α-GRP78-Akt signal pathway, suggesting that hypoxia-preconditioned MSCs might provide a therapeutic strategy for MSC-based therapies and that GRP78 represents a potential target for the development of functional MSCs.

  5. AAV delivery of GRP78/BiP promotes adaptation of human RPE cell to ER stress.

    Science.gov (United States)

    Ghaderi, Shima; Ahmadian, Shahin; Soheili, Zahra-Soheila; Ahmadieh, Hamid; Samiei, Shahram; Kheitan, Samira; Pirmardan, Ehsan R

    2018-02-01

    Adeno associated virus (AAV)-mediated gene delivery of GRP78 (78 kDa glucose-regulated protein) attenuates the condition of endoplasmic reticulum (ER) stress and prevents apoptotic loss of photoreceptors in Retinitis pigmentosa (RP) rats. In the current study we overexpressed Grp78 with the help of AAV-2 in primary human retinal pigmented epithelium (hRPE) cell cultures and examined its effect on cell response to ER stress. The purpose of this work was studying potential stimulating effect of GRP78 on adaptation/pro-survival of hRPE cells under ER stress, as an in vitro model for RPE degeneration. To investigate the effect of Grp78 overexpression on unfolded protein response (UPR) markers under ER stress, hRPE primary cultures were transduced by recombinant virus rAAV/Grp78, and treated with ER stressor drug, tunicamycin. Expression changes of four UPR markers including GRP78, PERK, ATF6α, and GADD153/CHOP, were assessed by real-time PCR and western blotting. We found that GRP78 has a great contribution in modulation of UPR markers to favor adaptive response in ER-stressed hRPE cells. In fact, GRP78 overexpression affected adaptation and apoptotic phases of early UPR, through enhancement of two master regulators/ER stress sensors (PERK and ATF6α) and down-regulation of a key pro-apoptotic cascade activator (GADD153/CHOP). Together these findings demonstrate the promoting effect of GRP78 on adaptation/pro-survival of hRPE cells under ER stress. This protein with anti-apoptotic actions in the early UPR and important role in cell fate regulation, can be recruited as a useful candidate for future investigations of RPE degenerative diseases. © 2017 Wiley Periodicals, Inc.

  6. Seleção de progênies do cruzamento entre cultivares de cafeeiros Icatu e Catuaí

    Directory of Open Access Journals (Sweden)

    Cristiano de Andrade Gomes

    2015-02-01

    Full Text Available Os programas de melhoramento devem ser dinâmicos e contínuos, de modo a disponibilizarem sempre novos cultivares de cafeeiros, com fatores de resistência complexos, que se constituam em eficientes barreiras para as novas raças fisiológicas do fungo H. vastatrix. Por essa razão, objetivou-se, com este trabalho, avaliar o comportamento agronômico, a resistência à ferrugem e a ocorrência de cercosporiose em progênies, provenientes do cruzamento dos grupos de cultivares Catuaí e Icatu, em dois ambientes de Minas Gerais. Os experimentos foram implantados nas Fazendas Experimentais da EPAMIG, em São Sebastião do Paraíso e Três Pontas - MG. O delineamento experimental adotado foi o látice quadrado 6 x 6, com 3 repetições. Nos dois ambientes, foram realizadas avaliações de produtividade, vigor vegetativo, percentagem de grãos com peneira 17 acima e reação à ferrugem e à cercosporiose, nos anos agrícolas 2009/2010 e 2010/2011. O desempenho das progênies avaliadas, para produtividade, em Três Pontas, foi superior quando comparado ao de São Sebastião do Paraíso. A progênie H 6-47-10 Cova 3 destacou-se entre as mais promissoras, sugerindo boas possibilidades de progresso genético na seleção.

  7. Diversidade genética entre progênies e matrizes de rambutan

    Directory of Open Access Journals (Sweden)

    Renata Aparecida de Andrade

    2012-06-01

    Full Text Available O rambutan é uma frutífera exótica que apresenta alto potencial de mercado, e suas mudas podem ser obtidas por sementes ou vegetativamente. A produção de mudas via sementes é rotineiramente feita no Estado de São Paulo, tendo-se alta variabilidade no pomar, além de demorar mais tempo para entrar em produção. Embora caracteres morfológicos sejam amplamente usados na diferenciação de variedades, as técnicas moleculares permitem a comparação e a identificação genética dos materiais. Diante disso, o presente trabalho foi realizado, comparando progênies e plantas-matrizes de rambutan, por fAFLP. As análises foram realizadas no Laboratório de Bioquímica de Microrganismos e Plantas, do Departamento de Tecnologia - Faculdade de Ciências Agrárias e Veterinárias - UNESP - Câmpus de Jaboticabal-SP, utilizando 06 plantas de rambutan, denominadas: A; B; C; D; E e F. Foram coletadas folhas de 15 plântulas oriundas de cada planta-matriz e realizou-se a extração de DNA, sendo as amostras quantificadas em biofotômetro, e os marcadores fAFLP, obtidos de acordo com o protocolo AFLP Plant Mapping Protocol (Applied Biosystems, utilizando as combinações de pares de primers: ACG/CAC; ACT/CAT; ACA/CTT e ACC/CTT. Pode ser concluído que o uso de marcadores moleculares é eficiente na distinção de materiais e na obtenção de distância genética; não é recomendada a obtenção de mudas via sementes quando a finalidade é a de instalação de pomar comercial.

  8. Variação da densidade básica da madeira de progênies de Eucalyptus urophylla em dois locais.

    Directory of Open Access Journals (Sweden)

    Maria Aparecida Mourão Brasil

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Estudou-se neste trabalho a variação da densidade básica da madeira entre procedências e progênies de eucalipto plantados em duas localidades. As progênies de Eucalyptus urophylla originárias da ilha de Timor na Indonésia foram plantadas em Belo Oriente (MG e Linhares (ES no delineamento de blocos compactos em famílias (compact family blocks com 3 repetições no espaçamento de 3,0 x 2,0m. Após 4 anos as  árvores  foram amostradas ao nível do DAP com a sonda Pressler e sua densidade básica determinada pelo método do máximo teor de umidade. Os resultados mostraram que as progênies tiveram  comportamento similar nos dois locais. A maior variação entre progênies ocorreu naquelas provenientes da região Oebaha II. Os valores médios de densidade básica* das progênies não foram diferentes das procedências brasileiras utilizadas como testemunhas no experimento.

  9. GRP-R expression in breast cancer as target for nuclear imaging and therapy, correlation with ER

    International Nuclear Information System (INIS)

    Dalm, S.U.; Melis, M.; Sieuwerts, A.M.; Martens, J.W.M.; Jong, M. de

    2015-01-01

    Full text of publication follows. Introduction: Breast cancer (BC) is a complex and heterogeneous disease: several molecular characteristics reflect subtypes, partly overlapping with therapeutic targets. Examples include the expression of the oestrogen receptor (ER), expressed in approximately 75 % of all breast cancer cases. Currently mammography, MRI, 99m Tc-Sestamibi scintigraphy, and 18 F-FDG PET are commonly used for diagnostic imaging to accurately localize BC. Since it has been reported that the gastrin releasing peptide receptor (GRP-R) is expressed in BC, targeting this receptor with radiolabeled GRP analogues might offer opportunities for SPECT/CT or PET/CT imaging as well as radionuclide therapy in BC. In this study GRP-R expression was determined in human BC specimens and BC cell lines and correlated with ER status. Methods: GRP-R mRNA levels of 90 human breast cancer specimens, with known ER status (48 ER-positive and 42 ER-negative) were determined using qRT-PCR in a Taqman Gene expression assay. Furthermore a panel of 21 BC cell lines characterized for ER expression (13 ER-positive, 8 ER-negative) was analysed for GRP-R expression at the protein level. Internalisation studies were performed with 10-9 M 111 In-AMBA (an receptor-agonist GRP analogue) for 1 hour and 15 minutes at 37 C. degrees. Thirteen of these BC cell lines were also analyzed for GRP-R expression at mRNA level using qRT-PCR. Results: Clinical BC specimens with high GRP-R mRNA level were all ER-positive, resulting in a significant positive correlation (p=0.03). Fifty-two percent of the analyzed BC cell lines showed the ability to internalize 111 In-AMBA, although high variation between cell lines was observed. GRP-R mRNA levels of the BC cell lines significantly correlated with the internalisation rate (p=0.0003), indicating that the amount of internalized 111 In-AMBA is partly determined by the level of receptor expression. However, no correlation was found between ER status and GRP

  10. A new polymorphism in the GRP78 is not associated with HBV invasion

    Science.gov (United States)

    Zhu, Xiao; Wang, Yi; Tao, Tao; Li, Dong-Pei; Lan, Fei-Fei; Zhu, Wei; Xie, Dan; Kung, Hsiang-Fu

    2009-01-01

    AIM: To examine the association between -86 bp (T > A) in the glucose-regulated protein 78 gene (GRP78) and hepatitis B virus (HBV) invasion. METHODS: DNA was genotyped for the single-nucleotide polymorphism by polymerase chain reaction followed by sequencing in a sample of 382 unrelated HBV carriers and a total of 350 sex- and age-matched healthy controls. Serological markers for HBV infection were determined by enzyme-linked immunosorbent assay kits or clinical chemistry testing. RESULTS: The distributions of allelotype and genotype in cases were not significantly different from those in controls. In addition, our findings suggested that neither alanine aminotransferase/hepatitis B e antigen nor HBV-DNA were associated with the allele/genotype variation in HBV infected individuals. CONCLUSION: -86 bp T > A polymorphism in GRP78 gene is not related to the clinical risk and acute exacerbation of HBV invasion. PMID:19842229

  11. GRP78 Protein Expression as Prognostic Values in Neoadjuvant Chemoradiotherapy and Laparoscopic Surgery for Locally Advanced Rectal Cancer.

    Science.gov (United States)

    Lee, Hee Yeon; Jung, Ji-Han; Cho, Hyun-Min; Kim, Sung Hwan; Lee, Kang-Moon; Kim, Hyung-Jin; Lee, Jong Hoon; Shim, Byoung Yong

    2015-10-01

    We investigated the relationships between biomarkers related to endoplasmic reticulum stress proteins (glucose-regulated protein of molecular mass 78 [GRP78] and Cripto-1 [teratocarcinoma-derived growth factor 1 protein]), pathologic response, and prognosis in locally advanced rectal cancer. All clinical stage II and III rectal cancer patients received 50.4 Gy over 5.5 weeks, plus 5-fluorouracil (400 mg/m(2)/day) and leucovorin (20 mg/m(2)/day) bolus on days 1 to 5 and 29 to 33, and surgery was performed at 7 to 10 weeks after completion of all therapies. Expression of GRP78 and Cripto-1 proteins was determined by immunohistochemistry and was assessed in 101 patients with rectal cancer treated with neoadjuvant chemoradiotherapy (CRT). High expression of GRP78 and Cripto-1 proteins was observed in 86 patients (85.1%) and 49 patients (48.5%), respectively. Low expression of GRP78 protein was associated with a significantly high rate of down staging (80.0% vs. 52.3%, respectively; p=0.046) and a significantly low rate of recurrence (0% vs. 33.7%, respectively; p=0.008) compared with high expression of GRP78 protein. Mean recurrence-free survival according to GRP78 expression could not be estimated because the low expression group did not develop recurrence events but showed a significant correlation with time to recurrence, based on the log rank method (p=0.007). GRP78 also showed correlation with overall survival, based on the log rank method (p=0.045). GRP78 expression is a predictive and prognostic factor for down staging, recurrence, and survival in rectal cancer patients treated with 5-fluorouracil and leucovorin neoadjuvant CRT.

  12. Machined GRP laminates for components in heavy electrical engineering and their use at very low temperatures

    International Nuclear Information System (INIS)

    Fuchs, H.

    1982-01-01

    Safe and economical components can be produced from machined GRP laminates. Matrix system, fibre reinforcement and elastic properties are described. Onset of damage and long-term properties are given with detailed charting of tests. Application of the laminate studies at stresses of up to half their short-term strength can be made, provided creep strain and its dependence on time and temperature are considered

  13. Role of prostate apoptosis response 4 in translocation of GRP78 from the endoplasmic reticulum to the cell surface of trophoblastic cells.

    Directory of Open Access Journals (Sweden)

    Marie Cohen

    Full Text Available Glucose-regulated protein 78 (GRP78 is an endoplasmic reticulum (ER molecular chaperone that belongs to the heat shock protein 70 family. GRP78 is also present on the cell surface membrane of trophoblastic cells, where it is associated with invasive or fusion properties of these cells. Impaired mechanism of GRP78 relocation from ER to the cell surface was observed in preeclamptic cytotrophoblastic cells (CTB and could take part in the pathogenesis of preeclampsia. In this study, we have investigated whether prostate apoptosis response 4 (Par-4, a protein identified as a partner of GRP78 relocation to the cell surface in prostate cancer cells, is present in trophoblastic cells and is involved in the translocation of GRP78 to the cell surface of CTB. Par-4 is indeed present in trophoblastic cells and its expression correlates with expression of membrane GRP78. Moreover, overexpression of Par-4 led to an increase of cell surface expression of GRP78 and decreased Par-4 gene expression reduced cell surface localization of GRP78 confirming a role of Par-4 in relocation of GRP78 from ER to the cell surface. Accordingly, invasive property was modified in these cells. In conclusion, we show that Par-4 is expressed in trophoblastic cells and is involved in transport of GRP78 to the cell surface and thus regulates invasive property of extravillous CTB.

  14. Role of the Escherichia coli grpE heat shock protein in the initiation of bacteriophage lambda DNA replication.

    Science.gov (United States)

    Osipiuk, J; Zylicz, M

    1991-01-01

    Initiation of replication of lambda DNA requires assembly of the proper nucleoprotein complex consisting of the lambda origin of replication-lambda O-lambda P-dnaB proteins. The dnaJ, dnaK and grpE heat shock proteins destabilize the lambda P-dnaB interaction in this complex permitting dnaB helicase to unwind lambda DNA near ori lambda sequence. First step of this disassembling reaction is the binding of dnaK protein to lambda P protein. In this report we examined the influence of dnaJ and grpE proteins on stability of the lambda P-dnaK complex. Our results show that grpE alone dissociates this complex, but both grpE and dnaJ together do not. These results suggest that, in the presence of grpE protein, dnaK protein has a higher affinity for lambda P protein complexed with dnaJ protein than in the situation where grpE protein is not used.

  15. Reversible thermal transition in GrpE, the nucleotide exchange factor of the DnaK heat-shock system.

    Science.gov (United States)

    Grimshaw, J P; Jelesarov, I; Schönfeld, H J; Christen, P

    2001-03-02

    DnaK, a Hsp70 acting in concert with its co-chaperones DnaJ and GrpE, is essential for Escherichia coli to survive environmental stress, including exposure to elevated temperatures. Here we explored the influence of temperature on the structure of the individual components and the functional properties of the chaperone system. GrpE undergoes extensive but fully reversible conformational changes in the physiologically relevant temperature range (transition midpoint at approximately 48 degrees C), as observed with both circular dichroism measurements and differential scanning calorimetry, whereas no thermal transitions occur in DnaK and DnaJ between 15 degrees C and 48 degrees C. The conformational changes in GrpE appear to be important in controlling the interconversion of T-state DnaK (ATP-liganded, low affinity for polypeptide substrates) and R-state DnaK (ADP-liganded, high affinity for polypeptide substrates). The rate of the T --> R conversion of DnaK due to DnaJ-triggered ATP hydrolysis follows an Arrhenius temperature dependence. In contrast, the rate of the R --> T conversion due to GrpE-catalyzed ADP/ATP exchange increases progressively less with increasing temperature and even decreases at temperatures above approximately 40 degrees C, indicating a temperature-dependent reversible inactivation of GrpE. At heat-shock temperatures, the reversible structural changes of GrpE thus shift DnaK toward its high-affinity R state.

  16. The rs391957 variant cis-regulating oncogene GRP78 expression contributes to the risk of hepatocellular carcinoma.

    Science.gov (United States)

    Zhu, Xiao; Zhang, Jinfang; Fan, Wenguo; Wang, Fang; Yao, Hong; Wang, Zifeng; Hou, Shengping; Tian, Yinghong; Fu, Weiming; Xie, Dan; Zhu, Wei; Long, Jun; Wu, Leijie; Zheng, Xuebao; Kung, Hsiangfu; Zhou, Keyuan; Lin, Marie C M; Luo, Hui; Li, Dongpei

    2013-06-01

    Glucose-regulated protein 78 (GRP78) is one of the most important responders to disease-related stress. We assessed the association of the promoter polymorphisms of GRP78 with risk of hepatocellular carcinoma (HCC) and GRP78 expression in a Chinese population. We examined 1007 patients undergoing diagnostic HCC and 810 unrelated healthy controls. Mechanisms by which the GRP78 promoter polymorphism modulates HCC risk and GRP78 levels were analyzed. The promoter haplotype and diplotype carrying rs391957 (-415bp) allele G and genotype GG was strongly associated with HCC risk. Luciferase reporter assays indicated that the promoter carrying rs391957 allele G (haplotype GCCd) showed increased activity in HepG2 cells and Hela cells. rs391957 was also shown to increase the affinity of the transcriptional activator Ets-2, the resistance to apoptosis, as well as cell instability in stressful microenvironment. Furthermore, compared with allele A, rs391957 allele G was associated with higher levels of GRP78 mRNA and protein in HCC tissues. These findings provided new insights into the pathogenesis of HCC and an unexpected effect of the interaction between rs391957 and Ets-2 on hepatocarcinogenesis, and especially supported the hypothesis that stress-related and evolutionarily conserved genetic variant(s) influencing transcriptional regulation could predict susceptibilities.

  17. [Functional analysis of Grp and Iris, the gag and env domesticated errantivirus genes, in the Drosophila melanogaster genome].

    Science.gov (United States)

    Makhnovskii, P A; Kuzmin, I V; Nefedova, L N; Kima, A I

    2016-01-01

    Drosophila melanogaster is the only invertebrate that contains endogenous retroviruses, which are called errantiviruses. Two domesticated genes, Grp and Iris, which originate from errantivirus gag and env, respectively, have been found in the D. melanogaster genome. The functions performed by the genes in Drosophila are still unclear. To identify the functions of domesticated gag and env in the D. melanogaster genome, expression of Iris and Grp was studied in strains differing by the presence or absence of the functional gypsy errantivirus. In addition, the expression levels were measured after injection of gram-positive and gram-negative bacteria, which activate different immune response pathways, and exposure to various abiotic stress factors. The presence of functional D. melanogaster retrovirus gypsy was found to increase the Grp expression level in somatic tissues of the carcass, while exerting no effect on the Iris expression level. Activation of the immune response in D. melanogaster by bacteria Bacillus cereus increased the Grp expression level and did not affect Iris expression. As for the effects of abiotic stress factors (oxidative stress, starvation, and heat and cold stress), the Grp expression level increased in response to starvation in D. melanogaster females, and the Iris expression level was downregulated in heat shock and oxidative stress. Based on the findings, Grp was assumed to play a direct role in the immune response in D. melanogaster; Iris is not involved in immune responses, but and apparently performs a cell function that is inhibited in stress.

  18. Potencial genético de progênies de feijão-caupi segregantes quanto ao tipo da inflorescência

    Directory of Open Access Journals (Sweden)

    Fábio Ribeiro Barros

    2011-02-01

    Full Text Available O objetivo deste trabalho foi avaliar o potencial genético produtivo de progênies de feijão-caupi (Vigna unguiculata segregantes quanto ao tipo da inflorescência. Foram avaliadas 68 progênies F4 obtidas dos retrocruzamentos: (TV x 5058-09C x Cacheado-roxo x TV x 5058-09C e (AU94-MOB-816 x Cacheado-roxo x AU94-MOB-816, com os genitores. Dois experimentos foram conduzidos no delineamento de blocos ao acaso, tendo-se avaliado 17 progênies, com quatro repetições, em parcelas subdivididas quanto à inflorescência: simples e composta. A análise estatística foi realizada por modelos mistos via procedimento REML/BLUP. As estimativas das variâncias genéticas foram significativas para todos os caracteres estudados. Os caracteres comprimento do pedúnculo, número médio de vagens por pedúnculo e floração inicial apresentaram alta variabilidade e expressão do componente genético para a inflorescência composta. As progênies de inflorescência simples apresentam potencial genético produtivo similar às progênies de inflorescência composta. As progênies resultantes do retrocruzamento (AU94-MOB-816 x Cacheado-roxo x AU94-MOB-816 são promissoras como estratégia para aumentar os níveis atuais de produtividade do feijão-caupi.

  19. Divergência genética entre progênies de polinização aberta de Pinus caribaea var. hondurensis a partir de caracteres quantitativos

    Directory of Open Access Journals (Sweden)

    Wanderley dos Santos

    2016-06-01

    Full Text Available Este trabalho foi realizado com o objetivo de estimar a divergência genética entre progênies de Pinus caribaea var. hondurensis, por meio de caracteres quantitativos. O experimento foi instalado em delineamento látice 10 x 10, triplo, com 100 tratamentos (96 progênies oriundas de polinização aberta de um pomar clonal da espécie e quatro testemunhas. Foram avaliados os caracteres: diâmetro a 1,30 m do solo, altura total de planta, volume cilíndrico, produção de resina total e resina por área de painel. Utilizou-se a distância generalizada de Mahalanobis (D2 e o método de otimização de Tocher. A maior distância genética observada entre as progênies foi de 100% (D2 = 65,51 e a menor foi de 0,09% (D2 = 0,15. O caractere volume foi o que mais contribuiu para a divergência genética entre os grupos avaliados. O agrupamento a partir do método de otimização de Tocher possibilitou a separação das progênies em quatro grupos, com concentração de 96,9% das progênies em um único grupo. Para que estas progênies possam ser incluídas em programas de melhoramento genético para produção de resina e madeira, cruzamentos controlados deverão ser priorizados entre indivíduos mais produtivos, que apresentaram maior divergência genética.

  20. Quicklook overview of model changes in Melcor 2.2: Rev 6342 to Rev 9496

    Energy Technology Data Exchange (ETDEWEB)

    Humphries, Larry L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-05-01

    MELCOR 2.2 is a significant official release of the MELCOR code with many new models and model improvements. This report provides the code user with a quick review and characterization of new models added, changes to existing models, the effect of code changes during this code development cycle (rev 6342 to rev 9496), a preview of validation results with this code version. More detailed information is found in the code Subversion logs as well as the User Guide and Reference Manuals.

  1. Rev-erbα and Rev-erbβ coordinately protect the circadian clock and normal metabolic function

    DEFF Research Database (Denmark)

    Bugge, Anne Skovsø; Feng, Dan; Everett, Logan J

    2012-01-01

    of binding sites across the genome, enriched near metabolic genes. Depletion of both Rev-erbs in liver synergistically derepresses several metabolic genes as well as genes that control the positive limb of the molecular clock. Moreover, deficiency of both Rev-erbs causes marked hepatic steatosis, in contrast......-autonomous clock as well as hepatic lipid metabolism. Mouse embryonic fibroblasts were rendered arrhythmic by depletion of both Rev-erbs. In mouse livers, Rev-erbβ mRNA and protein levels oscillate with a diurnal pattern similar to that of Rev-erbα, and both Rev-erbs are recruited to a remarkably similar set...

  2. Revised Safety Instruction 41 (IS41 REV.)

    CERN Multimedia

    SC Secretariat

    2005-01-01

    Please note that the Revised Safety Instruction No. 41 (IS41 REV.), entitled 'The use of plastic and other non-metallic materials at CERN with respect to fire safety and radiation resistance' is available on the web at the following url: https://edms.cern.ch/document/335806/LAST_RELEASED Paper copies can also be obtained from the SC Unit Secretariat, e-mail: sc.secretariat@cern.ch SC Secretariat

  3. Isthmin is a novel vascular permeability inducer that functions through cell-surface GRP78-mediated Src activation.

    Science.gov (United States)

    Venugopal, Shruthi; Chen, Mo; Liao, Wupeng; Er, Shi Yin; Wong, Wai-Shiu Fred; Ge, Ruowen

    2015-07-01

    Isthmin (ISM) is a recently identified 60 kDa secreted angiogenesis inhibitor. Two cell-surface receptors for ISM have been defined, the high-affinity glucose-regulated protein 78 kDa (GRP78) and the low-affinity αvβ5 integrin. As αvβ5 integrin plays an important role in pulmonary vascular permeability (VP) and ISM is highly expressed in mouse lung, we sought to clarify the role of ISM in VP. Recombinant ISM (rISM) dose-dependently enhances endothelial monolayer permeability in vitro and local dermal VP when administered intradermally in mice. Systemic rISM administration through intravenous injection leads to profound lung vascular hyperpermeability but not in other organs. Mechanistic investigations using molecular, biochemical approaches and specific chemical inhibitors revealed that ISM-GRP78 interaction triggers a direct interaction between GRP78 and Src, leading to Src activation and subsequent phosphorylation of adherens junction proteins and loss of junctional proteins from inter-endothelial junctions, resulting in enhanced VP. Dynamic studies of Src activation, VP and apoptosis revealed that ISM induces VP directly via Src activation while apoptosis contributes indirectly only after prolonged treatment. Furthermore, ISM is significantly up-regulated in lipopolysaccharide (LPS)-treated mouse lung. Blocking cell-surface GRP78 by systemic infusion of anti-GRP78 antibody significantly attenuates pulmonary vascular hyperpermeability in LPS-induced acute lung injury (ALI) in mice. ISM is a novel VP inducer that functions through cell-surface GRP78-mediated Src activation as well as induction of apoptosis. It induces a direct GRP78-Src interaction, leading to cytoplasmic Src activation. ISM contributes to pulmonary vascular hyperpermeability of LPS-induced ALI in mice. Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2015. For permissions please email: journals.permissions@oup.com.

  4. The current status of radiopharmacy laboratories in Turkey, conveniences to good radiopharmacy practice (GRP) and quality management systems (ISO)

    International Nuclear Information System (INIS)

    Atak, I.E.

    2004-01-01

    This study ha been conducted in the Radiopharmacy Laboratories of Nuclear Medicine departments of various hospitals and private nuclear medicine laboratories. A total of 35 laboratories from 7 regions of Turkey have been selected by layered sampling method from 131 Radiopharmacy Laboratories located in 30 different cities. During the study, a GRP investigation list with 67 questions and direct communication technique have been used. The aim was determine the current status of the Radiopharmacy Laboratories in general and the administration of radiopharmaceuticals on patients, and good practices in radiopharmacy and conformance with quality assurance systems. In this respect, questions have been asked to determine a) General status, b) Information level of lab workers regarding to the GRP and ISO concepts (i-Status of lab managers, ii- Responsibilities and knowledge of lab workers and iii- regarding to GRP and ISO-9000), c) Conditions of infrastructure, and lab services and its quality, d) Status of organizations. Results showed that only two of the 35 managers of laboratories were radiopharmacists, the rest were Nuclear Medicine specialists. There were less knowledge on GRP than ISO, the labs holding ISO certificate were in minority even though ISO is known concept, radiopharmacist were more knowledgeable in GRP while nuclear medicine specialists were in ISO, the labs with better GRP knowledge have better infrastructure, the GRP knowledge were better in the university and armed forces hospitals while ISO knowledge and certificates were more in private labs and hospitals, the armed forces hospitals better paraphernalia, practically almost all radiopharmaceutical kits were imported goods and there were important problems in quality control

  5. Introducing RevPASH: The Free Webtool Application

    Directory of Open Access Journals (Sweden)

    Peter Szende

    2014-10-01

    Full Text Available RevPASH (Revenue Per Available Seat Hour is an important measure that helps restaurant operators understand how efficiently each seat in a restaurant generates revenue. The RevPASH app is an easy-to-use web-tool that provides an operator with a quick way to input a few relevant numbers and calculate RevPASH.The application has the ability to compare RevPASH over different times, days, weeks, and months.

  6. Seleção de progênies femininas de mamoeiro para resistência a mancha-de-phoma via modelos mistos

    Directory of Open Access Journals (Sweden)

    Marcelo Vivas

    2014-12-01

    Full Text Available Genótipos crioulos são importantes fontes de genes de adaptação, rusticidade e resistência de plantas a doenças bióticas e a estresse ambiental. Avaliou-se neste trabalho o potencial genético de contribuição de genótipos crioulos de mamoeiro na resistência a mancha-de-phoma. O experimento, implantado em blocos casualizados com seis repetições, foi composto de 24 progênies dioicas de meios-irmãos de mamoeiro que segregaram para o sexo, sendo avaliadas apenas as plantas com flores femininas. Foram estimados os componentes de variância e o valor genético de progênies para incidência e severidade de mancha-de-phoma via modelos mistos. As estimativas de herdabilidade e acurácia seletiva permitiram predizer situação muito favorável à seleção de progênies resistentes, com destaque para as progênies STA-02, STA-04, STA-05, STA-06, STA-07, STA-11, STA-12, STA-16, STA-21 e STA-22, com valores negativos de efeitos genéticos aditivos tanto para severidade quanto para incidência da doença nas folhas.

  7. The Endoplasmic Reticulum Chaperone GRP78/BiP Modulates Prion Propagation in vitro and in vivo.

    Science.gov (United States)

    Park, Kyung-Won; Eun Kim, Gyoung; Morales, Rodrigo; Moda, Fabio; Moreno-Gonzalez, Ines; Concha-Marambio, Luis; Lee, Amy S; Hetz, Claudio; Soto, Claudio

    2017-03-23

    Prion diseases are fatal neurodegenerative disorders affecting several mammalian species, characterized by the accumulation of the misfolded form of the prion protein, which is followed by the induction of endoplasmic reticulum (ER) stress and the activation of the unfolded protein response (UPR). GRP78, also called BiP, is a master regulator of the UPR, reducing ER stress levels and apoptosis due to an enhancement of the cellular folding capacity. Here, we studied the role of GRP78 in prion diseases using several in vivo and in vitro approaches. Our results show that a reduction in the expression of this molecular chaperone accelerates prion pathogenesis in vivo. In addition, we observed that prion replication in cell culture was inversely related to the levels of expression of GRP78 and that both proteins interact in the cellular context. Finally, incubation of PrP Sc with recombinant GRP78 led to the dose-dependent reduction of protease-resistant PrP Sc in vitro. Our results uncover a novel role of GRP78 in reducing prion pathogenesis, suggesting that modulating its levels/activity may offer a novel opportunity for designing therapeutic approaches for these diseases. These findings may also have implications for other diseases involving the accumulation of misfolded proteins.

  8. The interplay between GRP78 expression and Akt activation in human colon cancer cells under celecoxib treatment.

    Science.gov (United States)

    Tian, Shaobo; Chang, Weilong; Du, Hansong; Bai, Jie; Sun, Zhenhai; Zhang, Qing; Wang, Hui; Zhu, Guangsheng; Tao, Kaixiong; Long, Yueping

    2015-10-01

    It has been reported previously that celecoxib shows antitumor effects in many types of cancers. Here, we detected its effects on DLD-1 and SW480 (two human colon cancer cell lines) and investigated the dynamic relationship between the 78-kDa glucose-regulatory protein (GRP78) and the phosphoinositide 3-kinase (PI3K)/Akt pathway. Gene expression was detected by real-time PCR and western blot analysis; the cytotoxicity was determined by the MTT assay and flow cytometry. First, the results showed that celecoxib induced cytotoxicity in a dose-dependent and time-dependent manner. Furthermore, we found the celecoxib-triggered unfolded protein response and the bidirectional regulation of Akt activation in both cell lines. Inhibiting the Akt activation by the PI3K inhibitor LY294002 markedly enhanced GRP78 expression. Besides, silencing the GRP78 expression regulated Akt activation in a time-dependent manner and increased the induction of the C/EBP homologous protein (CHOP) as well as considerably promoted celecoxib-induced apoptosis. In conclusion, these findings provide evidence that under the celecoxib treatment, GRP78 plays a protective role by modulating Akt activation and abrogating CHOP expression. However, Akt activation can provide a feedback loop to inhibit GRP78 expression. These studies can lead to novel therapeutic strategies for human colon cancer.

  9. Modelling of Safety Factors in the Design of GRP Composite Products

    DEFF Research Database (Denmark)

    Babu, B.J.C.; Prabhakaran, R.T. Durai; Lystrup, Aage

    2010-01-01

    as independent, while in real applications these factors may interact/influence each other. Following the concept developed by the authors, a simple graph theoretic model has been used to determine overall factor of safety. This is described with the help of an example and it has been demonstrated......An attempt has been made in this paper to arrive at the safety factor design of glass fibre reinforced polymer (GRP) composite products using graph theoretic model. In the conventional design and recommendations of the standards, these design factors affecting properties have been considered...

  10. The role of c-Src in the invasion and metastasis of hepatocellular carcinoma cells induced by association of cell surface GRP78 with activated α2M

    International Nuclear Information System (INIS)

    Zhao, Song; Li, Hongdan; Wang, Qingjun; Su, Chang; Wang, Guan; Song, Huijuan; Zhao, Liang; Luan, Zhidong; Su, Rongjian

    2015-01-01

    Emerging data have suggested that cell surface GRP78 is a multifunctional receptor and has been linked to proliferative and antiapoptotic signaling cascades. Activated α 2− macroglobin (α 2 M*) is a natural circulating ligand of cell surface GRP78. Association of cell surface GRP78 with α 2 M* is involved in the regulation of cell proliferation, survival and apoptosis in human cancers. The invasion and metastasis of HCC cells were examined using transwell and wound healing assay; Cell surface expression of GRP78 was detected by in cell western assay. Translocation of GRP78 from cytosol to cell surface was observed by transfection of GRP78-EGFP plus TRIRC-WGA staining. The levels of Src, phosphor-Src, FAK, phospho-FAK, EGFR, phospho-EGFR, phospho-Cortactin, phospho-Paxillin were determined by western blot. Cell surface expression of GRP78 in HCC tissue samples was observed by immunofluorescence. The distribution of Paxillin and Cortactin in HCC cells was also observed by immunofluorescence. The interaction between GRP78 and Src were detected by far-western blot, co-immunoprecipitation and GST pulldown. GRP78 mRNA was detected by RT-PCR. In the current study, we showed that association of cell surface GRP78 with α 2 M* stimulated the invasion and metastasis of HCC. Cell surface GRP78 could interact directly with c-Src, promoted the phosphorylation of c-Src at Y416. Inhibition of the tyrosine kinase activity of c-Src with PP2 reverted the stimulatory effect caused by association of cell surface GRP78 with α 2 M*. Moreover, association of cell surface GRP78 with α 2 M* facilitates the interaction between EGFR and c-Src and consequently phosphorylated EGFR at Y1101 and Y845, promoting the invasion and metastasis of HCCs. However, inhibition of the tyrosine kinase of c-Src do not affect the interaction between EGFR and Src. c-Src plays a critical role in the invasion and metastasis of HCC induced by association of cell surface GRP78 with α 2 M*. Cell surface GRP

  11. Rev 12 as a theology of history

    OpenAIRE

    Joanna Nowińska

    2009-01-01

    The Biblical writers notice history as the space of God’s rule. He is the director, who comes into contact with human being through signs – events and words, and also He is the history’s perpetuum mobile. Rev 12 specifically reflect nowadays and the previous in the context of the whole world’s vision and mix the reference to facts (lack of the temple, ark, faithful people, horrible experiences, the death danger), places (the temple, a desert), persons (the Child-Ruler, Michael) with the Old T...

  12. Rev 12 as a theology of history

    Directory of Open Access Journals (Sweden)

    Joanna Nowińska

    2009-12-01

    Full Text Available The Biblical writers notice history as the space of God’s rule. He is the director, who comes into contact with human being through signs – events and words, and also He is the history’s perpetuum mobile. Rev 12 specifically reflect nowadays and the previous in the context of the whole world’s vision and mix the reference to facts (lack of the temple, ark, faithful people, horrible experiences, the death danger, places (the temple, a desert, persons (the Child-Ruler, Michael with the Old Testament figurative exposing, a typical one for such a book (the Woman with Child, the heaven, the dragon, enriched with a lot of symbols (a crown, a horn, the moon under feet. God’s interference into World history is presented through lightning, voices, thunder, an earthquake and great hail, that stress His power and supremacy. The biblical writer refers to events, which make place whole the time in the natural- and supernatural space, like: the war between God and evil, persecutions, hiding, God’s care of men.  The specific literary structure of Rev 12, contrary to the other parts of that book, seem to help to put an accent for the fundamental truths for transcendental theology of history of which the most important is the eternal rule of God and only accidental, finished in the time perspective of Satan’s position.

  13. Suppression of atherosclerosis by synthetic REV-ERB agonist

    Energy Technology Data Exchange (ETDEWEB)

    Sitaula, Sadichha [Department of Molecular Therapeutics, The Scripps Research Institute, Jupiter, FL 33458 (United States); Billon, Cyrielle [Department of Pharmacological & Physiological Science, Saint Louis University School of Medicine, St. Louis, MO 63104 (United States); Kamenecka, Theodore M.; Solt, Laura A. [Department of Molecular Therapeutics, The Scripps Research Institute, Jupiter, FL 33458 (United States); Burris, Thomas P., E-mail: burristp@slu.edu [Department of Pharmacological & Physiological Science, Saint Louis University School of Medicine, St. Louis, MO 63104 (United States)

    2015-05-08

    The nuclear receptors for heme, REV-ERBα and REV-ERBβ, play important roles in the regulation of metabolism and inflammation. Recently it was demonstrated that reduced REV-ERBα expression in hematopoetic cells in LDL receptor null mice led to increased atherosclerosis. We sought to determine if synthetic REV-ERB agonists that we have developed might have the ability to suppress atherosclerosis in this model. A previously characterized synthetic REV-ERB agonist, SR9009, was used to determine if activation of REV-ERB activity would affect atherosclerosis in LDL receptor deficient mice. Atherosclerotic plaque size was significantly reduced (p < 0.05) in mice administered SR9009 (100 mg/kg) for seven weeks compared to control mice (n = 10 per group). SR9009 treatment of bone marrow-derived mouse macrophages (BMDM) reduced the polarization of BMDMs to proinflammatory M1 macrophage while increasing the polarization of BMDMs to anti-inflammatory M2 macrophages. Our results suggest that pharmacological targeting of REV-ERBs may be a viable therapeutic option for treatment of atherosclerosis. - Highlights: • Synthetic REV-ERB agonist treatment reduced atherosclerosis in a mouse model. • Pharmacological activation of REV-ERB decreased M1 macrophage polarization. • Pharmacological activation of REV-ERB increased M2 macrophage polarization.

  14. Discovery of a novel target for the dysglycemic chromogranin A fragment pancreastatin: interaction with the chaperone GRP78 to influence metabolism.

    Directory of Open Access Journals (Sweden)

    Nilima Biswas

    Full Text Available RATIONALE: The chromogranin A-derived peptide pancreastatin (PST is a dysglycemic, counter-regulatory peptide for insulin action, especially in liver. Although previous evidence for a PST binding protein has been reported, such a receptor has not been identified or sequenced. METHODS AND RESULTS: We used ligand affinity to purify the PST target, with biotinylated human PST (hCHGA273-301-amide as "bait" and mouse liver homogenate as "prey", and identified GRP78 (a.k.a. "78 kDa Glucose Regulated Protein", HSPA5, BIP as a major interacting partner of PST. GRP78 belongs to the family of heat shock proteins (chaperones, involved in several cellular processes including protein folding and glucose metabolism. We analyzed expression of GRP78 in the absence of PST in a mouse knockout model lacking its precursor CHGA: hepatic transcriptome data revealed global over-expression of not only GRP78 but also other heat shock transcripts (of the "adaptive UPR" in CHGA(-/- mice compared to wild-type (+/+. By contrast, we found a global decline in expression of hepatic pro-apoptotic transcripts in CHGA(-/- mice. GRP78's ATPase enzymatic activity was dose-dependently inhibited by PST (IC50∼5.2 µM. PST also inhibited the up-regulation of GRP78 expression during UPR activation (by tunicamycin in hepatocytes. PST inhibited insulin-stimulated glucose uptake in adipocytes, and increased hepatic expression of G6Pase (the final step in gluconeogenesis/glycogenolysis. In hepatocytes not only PST but also other GRP78-ATPase inhibitors (VER-155008 or ADP increased G6Pase expression. GRP78 over-expression inhibited G6Pase expression in hepatocytes, with partial restoration by GRP78-ATPase inhibitors PST, VER-155008, or ADP. CONCLUSIONS: Our results indicate that an unexpected major hepatic target of PST is the adaptive UPR chaperone GRP78. PST not only binds to GRP78 (in pH-dependent fashion, but also inhibits GRP78's ATPase enzymatic activity, and impairs its biosynthetic

  15. Expression, purification, crystallization and preliminary X-ray characterization of the GRP carbohydrate-recognition domain from Homo sapiens

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, Dongwen; Sun, Jianping; Zhao, Wei; Zhang, Xiao; Shi, Yunyu; Teng, Maikun, E-mail: mkteng@ustc.edu.cn; Niu, Liwen, E-mail: mkteng@ustc.edu.cn [Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027 (China); Key Laboratory of Structural Biology, Chinese Academy of Sciences, 96 Jinzhai Road, Hefei, Anhui 230027 (China); Dong, Yuhui; Liu, Peng [Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yuquan Road, Beijing 100039 (China); Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027 (China)

    2006-05-01

    The CRD domain of GRP from H. sapiens has been expressed, purified and crystallized and X-ray diffraction data have been collected to a resolution of 2.0 Å. Galectins are a family of animal lectins which share similar carbohydrate-recognition domains (CRDs) and an affinity for β-galactosides. A novel human galectin-related protein named GRP (galectin-related protein; previously known as HSPC159) comprises only one conserved CRD with 38 additional N-terminal residues. The C-terminal fragment of human GRP (GRP-C; residues 38–172) containing the CRD has been expressed and purified. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 2% PEG 400 and 2M ammonium sulfate in 100 mM Tris–HCl buffer pH 7.5. Diffraction data were collected to a resolution limit of 2.0 Å at beamline 3W1A of Beijing Synchrotron Radiation Facility at 100 K. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 123.07, b = 96.67, c = 61.56 Å, β = 118.72°. The estimated Matthews coefficient was 2.6 Å{sup 3} Da{sup −1}, corresponding to 51.8% solvent content.

  16. Expression, purification, crystallization and preliminary X-ray characterization of the GRP carbohydrate-recognition domain from Homo sapiens

    International Nuclear Information System (INIS)

    Zhou, Dongwen; Sun, Jianping; Zhao, Wei; Zhang, Xiao; Shi, Yunyu; Teng, Maikun; Niu, Liwen; Dong, Yuhui; Liu, Peng

    2006-01-01

    The CRD domain of GRP from H. sapiens has been expressed, purified and crystallized and X-ray diffraction data have been collected to a resolution of 2.0 Å. Galectins are a family of animal lectins which share similar carbohydrate-recognition domains (CRDs) and an affinity for β-galactosides. A novel human galectin-related protein named GRP (galectin-related protein; previously known as HSPC159) comprises only one conserved CRD with 38 additional N-terminal residues. The C-terminal fragment of human GRP (GRP-C; residues 38–172) containing the CRD has been expressed and purified. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 2% PEG 400 and 2M ammonium sulfate in 100 mM Tris–HCl buffer pH 7.5. Diffraction data were collected to a resolution limit of 2.0 Å at beamline 3W1A of Beijing Synchrotron Radiation Facility at 100 K. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 123.07, b = 96.67, c = 61.56 Å, β = 118.72°. The estimated Matthews coefficient was 2.6 Å 3 Da −1 , corresponding to 51.8% solvent content

  17. GRP94: An HSP90-like protein specialized for protein folding and quality control in the endoplasmic reticulum

    DEFF Research Database (Denmark)

    Marzec, Michal; Eletto, Davide; Argon, Yair

    2012-01-01

    Glucose-regulated protein 94 is the HSP90-like protein in the lumen of the endoplasmic reticulum and therefore it chaperones secreted and membrane proteins. It has essential functions in development and physiology of multicellular organisms, at least in part because of this unique clientele. GRP94...

  18. Induction of endoplasmic reticulum stress by deletion of Grp78 depletes Apc mutant intestinal epithelial stem cells.

    Science.gov (United States)

    van Lidth de Jeude, J F; Meijer, B J; Wielenga, M C B; Spaan, C N; Baan, B; Rosekrans, S L; Meisner, S; Shen, Y H; Lee, A S; Paton, J C; Paton, A W; Muncan, V; van den Brink, G R; Heijmans, J

    2017-06-15

    Intestinal epithelial stem cells are highly sensitive to differentiation induced by endoplasmic reticulum (ER) stress. Colorectal cancer develops from mutated intestinal epithelial stem cells. The most frequent initiating mutation occurs in Apc, which results in hyperactivated Wnt signalling. This causes hyperproliferation and reduced sensitivity to chemotherapy, but whether these mutated stem cells are sensitive to ER stress induced differentiation remains unknown. Here we examined this by generating mice in which both Apc and ER stress repressor chaperone Grp78 can be conditionally deleted from the intestinal epithelium. For molecular studies, we used intestinal organoids derived from these mice. Homozygous loss of Apc alone resulted in crypt elongation, activation of the Wnt signature and accumulation of intestinal epithelial stem cells, as expected. This phenotype was however completely rescued on activation of ER stress by additional deletion of Grp78. In these Apc-Grp78 double mutant animals, stem cells were rapidly lost and repopulation occurred by non-mutant cells that had escaped recombination, suggesting that Apc-Grp78 double mutant stem cells had lost self-renewal capacity. Although in Apc-Grp78 double mutant mice the Wnt signature was lost, these intestines exhibited ubiquitous epithelial presence of nuclear β-catenin. This suggests that ER stress interferes with Wnt signalling downstream of nuclear β-catenin. In conclusion, our findings indicate that ER stress signalling results in loss of Apc mutated intestinal epithelial stem cells by interference with the Wnt signature. In contrast to many known inhibitors of Wnt signalling, ER stress acts downstream of β-catenin. Therefore, ER stress poses a promising target in colorectal cancers, which develop as a result of Wnt activating mutations.

  19. Residues Phe103 and Phe149 are critical for the co-chaperone activity of Bacillus licheniformis GrpE.

    Science.gov (United States)

    Lin, Min-Guan; Chi, Meng-Chun; Chen, Bo-En; Wang, Tzu-Fan; Lo, Huei-Fen; Lin, Long-Liu

    2015-01-01

    A tryptophan-free Bacillus licheniformis nucleotide exchange factor (BlGrpE) and its Trp mutants (F70W, F103W, F149W, F70/103W, F70/149W, F103/149W and F70/103/149W) were over-expressed and purified to near homogeneity. Simultaneous addition of B. licheniformis DnaJ, NR-peptide and individual variants synergistically stimulated the ATPase activity of a recombinant DnaK (BlDnaK) from the same bacterium by 3.1-14.7-fold, which are significantly lower than the synergistic stimulation (18.9-fold) of BlGrpE. Protein-protein interaction analysis revealed that Trp mutants relevant to amino acid positions 103 and 149 lost the ability to bind BlDnaK. Circular dichroism measurements indicate that F70W displayed a comparable level of secondary structure to that of BlGrpE, and the wild-type protein and the Trp mutants as well all experienced a reversible behavior of thermal denaturation. Guanidine hydrochloride (GdnHCl)-induced unfolding transition for BlGrpE was calculated to be 1.25 M corresponding to ΔG(N-U) of 4.29 kcal/mol, whereas the unfolding transitions of mutant proteins were in the range of 0.77-1.31 M equivalent to ΔG(N-U) of 2.41-4.14 kcal/mol. Taken together, the introduction of tryptophan residue, especially at positions 103 and 149, into the primary structure of BlGrpE has been proven to be detrimental to structural integrity and proper function of the protein. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. DiagTest3Grp: An R Package for Analyzing Diagnostic Tests with Three Ordinal Groups

    Directory of Open Access Journals (Sweden)

    Jingqin Luo

    2012-10-01

    Full Text Available Medical researchers endeavor to identify potentially useful biomarkers to develop marker-based screening assays for disease diagnosis and prevention. Useful summary measures which properly evaluate the discriminative ability of diagnostic markers are critical for this purpose. Literature and existing software, for example, R packages nicely cover summary measures for diagnostic markers used for the binary case (e.g., healthy vs. diseased. An intermediate population at an early disease stage usually exists between the healthy and the fully diseased population in many disease processes. Supporting utilities for three-group diagnostic tests are highly desired and important for identifying patients at the early disease stage for timely treatments. However, application packages which provide summary measures for three ordinal groups are currently lacking. This paper focuses on two summary measures of diagnostic accuracy—volume under the receiver operating characteristic surface and the extended Youden index, with three diagnostic groups. We provide the R package DiagTest3Grp to estimate, under both parametric and nonparametric assumptions, the two summary measures and the associated variances, as well as the optimal cut-points for disease diagnosis. An omnibus test for multiple markers and a Wald test for two markers, on independent or paired samples, are incorporated to compare diagnostic accuracy across biomarkers. Sample size calculation under the normality assumption can be performed in the R package to design future diagnostic studies. A real world application evaluating the diagnostic accuracy of neuropsychological markers for Alzheimer’s disease is used to guide readers through step-by-step implementation of DiagTest3Grp to demonstrate its utility.

  1. A Histidine pH sensor regulates activation of the Ras-specific guanine nucleotide exchange factor RasGRP1.

    Science.gov (United States)

    Vercoulen, Yvonne; Kondo, Yasushi; Iwig, Jeffrey S; Janssen, Axel B; White, Katharine A; Amini, Mojtaba; Barber, Diane L; Kuriyan, John; Roose, Jeroen P

    2017-09-27

    RasGRPs are guanine nucleotide exchange factors that are specific for Ras or Rap, and are important regulators of cellular signaling. Aberrant expression or mutation of RasGRPs results in disease. An analysis of RasGRP1 SNP variants led to the conclusion that the charge of His 212 in RasGRP1 alters signaling activity and plasma membrane recruitment, indicating that His 212 is a pH sensor that alters the balance between the inactive and active forms of RasGRP1. To understand the structural basis for this effect we compared the structure of autoinhibited RasGRP1, determined previously, to those of active RasGRP4:H-Ras and RasGRP2:Rap1b complexes. The transition from the autoinhibited to the active form of RasGRP1 involves the rearrangement of an inter-domain linker that displaces inhibitory inter-domain interactions. His 212 is located at the fulcrum of these conformational changes, and structural features in its vicinity are consistent with its function as a pH-dependent switch.

  2. The essential Escherichia coli msgB gene, a multicopy suppressor of a temperature-sensitive allele of the heat shock gene grpE, is identical to dapE.

    OpenAIRE

    Wu, B; Georgopoulos, C; Ang, D

    1992-01-01

    The grpE gene product is one of three Escherichia coli heat shock proteins (DnaK, DnaJ, and GrpE) that are essential for both bacteriophage lambda DNA replication and bacterial growth at all temperatures. In an effort to determine the role of GrpE and to identify other factors that it may interact with, we isolated multicopy suppressors of the grpE280 point mutation, as judged by their ability to reverse the temperature-sensitive phenotype of grpE280. Here we report the characterization of on...

  3. High expression of GRP78/BiP as a novel predictor of favorable outcomes in patients with advanced thymic carcinoma.

    Science.gov (United States)

    Miura, Yosuke; Kaira, Kyoichi; Sakurai, Reiko; Imai, Hisao; Tomizawa, Yoshio; Sunaga, Noriaki; Minato, Koichi; Hisada, Takeshi; Oyama, Tetsunari; Yamada, Masanobu

    2017-10-01

    Glucose-regulated protein (GRP) 78/immunoglobulin heavy chain binding protein (BiP) is a member of the endoplasmic reticulum chaperone family, and its role in various types of human malignancies has recently been investigated. However, the clinicopathological characteristics of GRP78/BiP in advanced thymic carcinoma (ATC) remain unknown. We aimed to examine the relationship between GRP78/BiP expression and the clinical outcomes of ATC patients. Thirty-four patients with ATC receiving combination chemotherapy at three institutions between April 1998 and April 2014 were enrolled in this study. We retrospectively collected patient characteristics such as therapeutic efficacy, pathological findings, and survival data from their medical records. We performed immunohistochemical analysis to evaluate the expression of GRP78/BiP in tumor specimens obtained from surgical resection or biopsy. This study included 21 men (68%) and 13 women (32%) with a median age of 62 years (range 36-75 years). GRP78/BiP overexpression was observed in 65% of the patients (22 of 34 patients). There was no correlation between GRP78/BiP expression and any patient characteristic. Patients with a high level of GRP78/BiP expression had significantly longer overall survival (OS) compared to those with a low level (46.2 vs. 16.8 months, p = 0.04). Multivariate analysis demonstrated that a high level of GRP78/BiP expression was an independent prognostic factor for prolonged OS. Our findings indicate that the overexpression of GRP78/BiP is a novel predictor of favorable outcomes in patients with ATC who receive combination chemotherapy.

  4. Administrative Circular No. 14 (Rev. 2)

    CERN Document Server

    HR Department

    2007-01-01

    The HR Department wishes to draw the attention of members of the personnel to a number of amendments to Administrative Circular No. 14 (Rev. 2) entitled "Protection of members of the personnel against the financial consequences of illness, accident and disability" which came into force on 1st July 2006 (cf. Weekly Bulletin of 14 and 21 August 2006). Occupational Accident Declaration Form (HS50) https://cern.ch/service-procedures/AdminMan/Forms/HS50E.doc •\tIt must be completed within 10 working days of the date on which the accident occurred (§ 29.2.1), unless the person concerned is materially unable to meet this deadline. • The completed formula must be accompanied by a medical certificate giving details of any bodily injuries resulting from the accident (Annex 1, § 5). The medical certificate must be obtained from the doctor who has been consulted for that purpose. Benefits resulting from illnesses and accidents Medical treatment will cease to be reimbursed under ...

  5. Physical exercise alleviates ER stress in obese humans through reduction in the expression and release of GRP78 chaperone.

    Science.gov (United States)

    Khadir, Abdelkrim; Kavalakatt, Sina; Abubaker, Jehad; Cherian, Preethi; Madhu, Dhanya; Al-Khairi, Irina; Abu-Farha, Mohamed; Warsame, Samia; Elkum, Naser; Dehbi, Mohammed; Tiss, Ali

    2016-09-01

    Perturbation of the endoplasmic reticulum (ER) homeostasis has emerged as one of the prominent features of obesity and diabetes. This occurs when the adaptive unfolded protein response (UPR) fails to restore ER function in key metabolic tissues. We previously reported increased inflammation and impaired heat shock response (HSR) in obese human subjects that were restored by physical exercise. Here, we investigated the status of ER stress chaperone; glucose-regulated protein 78 (GRP78) and its downstream UPR pathways in human obese, and their modulation by a supervised 3-month physical exercise. Subcutaneous adipose tissue (SAT) and blood samples were collected from non-diabetic adult human lean (n=40) and obese (n=40, at baseline and after 3months of physical exercise). Transcriptomic profiling was used as a primary screen to identify differentially expressed genes and it was carried out on SAT samples using the UPR RT(2) Profiler PCR Array. Conventional RT-PCR, immunohistochemistry, immunofluorescence, Western blot and ELISA were used to validate the transcriptomic data. Correlation analyses with the physical, clinical and biochemical outcomes were performed using Pearson's rank correlation coefficient. Levels of GRP78 and its three downstream UPR arms; activating transcription factor-6 (ATF6), inositol-requiring enzyme-1α (IRE1α) and protein kinase RNA-like endoplasmic reticulum kinase (PERK) were increased in obese subjects. More interestingly, higher levels of circulating GRP78 protein were found in obese compared to lean subjects which correlated negatively with maximum oxygen uptake (VO2 Max) but positively with high-sensitivity C-reactive protein (hsCRP) and obesity indicators such as BMI, percentage body fat (PBF) and waist circumference. GRP78 increased secretion in obese was further confirmed in vitro using 3T3-L1 preadipocyte cells under ER stress. Finally, we showed that physical exercise significantly attenuated the expression and release of GRP78

  6. Biallelic inactivation of REV7 is associated with Fanconi anemia.

    Science.gov (United States)

    Bluteau, Dominique; Masliah-Planchon, Julien; Clairmont, Connor; Rousseau, Alix; Ceccaldi, Raphael; Dubois d'Enghien, Catherine; Bluteau, Olivier; Cuccuini, Wendy; Gachet, Stéphanie; Peffault de Latour, Régis; Leblanc, Thierry; Socié, Gérard; Baruchel, André; Stoppa-Lyonnet, Dominique; D'Andrea, Alan D; Soulier, Jean

    2016-09-01

    Fanconi anemia (FA) is a recessive genetic disease characterized by congenital abnormalities, chromosome instability, progressive bone marrow failure (BMF), and a strong predisposition to cancer. Twenty FA genes have been identified, and the FANC proteins they encode cooperate in a common pathway that regulates DNA crosslink repair and replication fork stability. We identified a child with severe BMF who harbored biallelic inactivating mutations of the translesion DNA synthesis (TLS) gene REV7 (also known as MAD2L2), which encodes the mutant REV7 protein REV7-V85E. Patient-derived cells demonstrated an extended FA phenotype, which included increased chromosome breaks and G2/M accumulation upon exposure to DNA crosslinking agents, γH2AX and 53BP1 foci accumulation, and enhanced p53/p21 activation relative to cells derived from healthy patients. Expression of WT REV7 restored normal cellular and functional phenotypes in the patient's cells, and CRISPR/Cas9 inactivation of REV7 in a non-FA human cell line produced an FA phenotype. Finally, silencing Rev7 in primary hematopoietic cells impaired progenitor function, suggesting that the DNA repair defect underlies the development of BMF in FA. Taken together, our genetic and functional analyses identified REV7 as a previously undescribed FA gene, which we term FANCV.

  7. Analysis of translesion DNA synthesis activity of the human REV1-REV7 complex, which is a key player in radiation-induced mutagenesis

    International Nuclear Information System (INIS)

    Masuda, Y.; Masuda, K.; Kamiya, K.

    2003-01-01

    Full text: Ionizing radiation frequently causes oxidative DNA damage in cells. It has been suggested that functions of the REV1 and REV7 genes are induction of mutations and prevention of cell death caused by ionizing radiation. With yeast Saccharomyces cerevisiae, results from a variety of investigations have demonstrated that the REV genes play a major role in induction of mutations through replication processes which directly copy the damaged DNA template during DNA replication. However, in higher eucaryotes, functions of homologues are poorly understood and appear somewhat different from the yeast case. It has been suggested that human REV1 interacts with human REV7, this being specific to higher eucaryotes. Here we show that purified human REV1 and REV7 proteins form a heterodimer in solution, which is stable through intensive purification steps. Results from biochemical analysis of the transferase reactions of the REV1-REV7 complex demonstrated, in contrast to the case of yeast Rev3 whose polymerase activity is stimulated by assembly with yeast Rev7, that human REV7 did not influence the stability, substrate specificity or kinetic parameters of the transferase reactions of REV1 protein. A possible molecular role of the REV7 subunit may be to help assembly of the REV1 protein to a large complex containing REV3 and/or other DNA polymerases in higher eucaryotes

  8. Analysis of the influence of subcellular localization of the HIV Rev protein on Rev-dependent gene expression by multi-fluorescence live-cell imaging

    International Nuclear Information System (INIS)

    Wolff, Horst; Hadian, Kamyar; Ziegler, Manja; Weierich, Claudia; Kramer-Hammerle, Susanne; Kleinschmidt, Andrea; Erfle, Volker; Brack-Werner, Ruth

    2006-01-01

    The human immunodeficiency virus Rev protein is a post-transcriptional activator of HIV gene expression. Rev is a nucleocytoplasmic shuttle protein that displays characteristic nuclear/nucleolar subcellular localization in various cell lines. Cytoplasmic localization of Rev occurs under various conditions disrupting Rev function. The goal of this study was to investigate the relationship between localization of Rev and its functional activity in living cells. A triple-fluorescent imaging assay, called AQ-FIND, was established for automatic quantitative evaluation of nucleocytoplasmic distribution of fluorescently tagged proteins. This assay was used to screen 500 rev genes generated by error-prone PCR for Rev mutants with different localization phenotypes. Activities of the Rev mutants were determined with a second quantitative, dual-fluorescent reporter assay. In HeLa cells, the majority of nuclear Rev mutants had activities similar to wild-type Rev. The activities of Rev mutants with abnormal cytoplasmic localization ranged from moderately impaired to nonfunctional. There was no linear correlation between subcellular distribution and levels of Rev activity. In astrocytes, nuclear Rev mutants showed similar impaired activities as the cytoplasmic wild-type Rev. Our data suggest that steady-state subcellular localization is not a primary regulator of Rev activity but may change as a secondary consequence of altered Rev function. The methodologies described here have potential for studying the significance of subcellular localization for functions of other regulatory factors

  9. Serum GRP78 as a Tumor Marker and Its Prognostic Significance in Non-Small Cell Lung Cancers: A Retrospective Study

    Directory of Open Access Journals (Sweden)

    Xiao Ma

    2015-01-01

    Full Text Available Introduction. Glucose-regulated protein 78 (78 kDa, GRP78, which is also known as immunoglobulin heavy chain binding protein (BIP, is a major chaperone in the endoplasmic reticulum (ER. The expression and clinical significance of GRP78 in the serum of non-small cell lung cancer patients have not yet been clearly described. The aims of the present study were to investigate the expression of GRP78 in the serum of non-small cell lung cancer patients, the relationships with clinicopathological parameters, and the potential implications for survival. Patients and Methods. A total of 163 peripheral blood samples from non-small cell lung cancer patients were prospectively collected at the Department of Thoracic Surgery, Fudan University Shanghai Cancer, China. Clinical characteristics data, including age, gender, stage, overall survival (OS time, and relapse-free survival (RFS time, were also collected. Serum GRP78 levels were measured using a commercially available ELISA kit. The associations between GRP78 levels and clinicopathological characteristics and survival were examined using Student’s t-test, Kaplan-Meier, or Cox regression analyses. Results. The mean ± standard error (SE value of GRP78 was 326.5 ± 49.77 pg/mL. This level was significantly lower compared with the level in late-stage non-small cell lung cancer patients (1227 ± 223.6, p=0.0001. There were no significant correlations with the clinicopathological parameters. No significant difference was found between high GRP78 expression and low GRP78 expression with regard to RFS (p=0.1585. However, the OS of patients with higher GRP78 expression was significantly poorer (p=0.0334. Conclusions. GRP78 was expressed in non-small cell lung cancer patients and was highly enriched in late-stage lung cancer. GRP78 may have an important role in the carcinogenesis of non-small cell lung cancer and may be a prognostic marker for non-small cell lung cancer.

  10. Wave power plant at Horns Rev. Screening[Denmark]; Boelgekraftanlaeg ved Horns Rev. Screening

    Energy Technology Data Exchange (ETDEWEB)

    Soerensen, Hans C.; Nielsen, Kim; Steenstrup, P.R.; Friis-Madsen, E.; Wigant, L.

    2005-12-15

    The objective for the analysis has been to establish data for the sea at Horns Rev wind farm in the North Sea in order to assess the opportunity for using the site as test site for demonstration of wave energy devices exemplified by three different devices under development in Denmark. For comparison alternative sites like Hanstholm, Samsoe and Nissum Bredning are also assessed as well as the test centre EMEC at the Orkney Islands and the proposed test site Wave Hub at the north coast of Cornwall. The analysis shows that it is possible without major technical problems to connect 2-4 MW power generated by 3 different wave energy devices (AquaBuOY, Wave Star Energy and Wave Dragon) to the wind farm at Horns Rev (www.hornsrev.dk). The expenses for connection and regulation within the wind farm is about 200,000 DKK (30,00 EURO). On top of this comes the cost for individual sub sea cable connection to the wave devices, pull in of the sub sea cable through the existing J-tube in turbine T04 and the necessary regulation/control system in the individual wave devices to avoid damaging the power system in case of too high production. The analysis of the co-production of wind and wave power is dealt with in a separate report which shows that over a time period of half to one hour the time variation for wind generated electricity is 3 times as large as for wave energy generated power based on the actual measurement at Horns Rev. Further on the analysis shows that the wave generated power is more predictable than wind energy generated power as the power from the waves first is present about 2 hours after the wind is acting and last for 3 to 6 hours after the wind dies out; 6 to 12 hours with wind from west. The time is off course strongly depending of the direction of the wind i.e. the fetch. As this special report has a more general scope than the analysis as such it is reported in English (Annex Report II). The analysis shows that it is up to the individual device developer

  11. Repositioning of Verrucosidin, a Purported Inhibitor of Chaperone Protein GRP78, as an Inhibitor of Mitochondrial Electron Transport Chain Complex I

    Science.gov (United States)

    Gonzalez, Reyna; Pao, Peng-Wen; Hofman, Florence M.; Chen, Thomas C.; Louie, Stan G.; Pirrung, Michael C.; Schönthal, Axel H.

    2013-01-01

    Verrucosidin (VCD) belongs to a group of fungal metabolites that were identified in screening programs to detect molecules that preferentially kill cancer cells under glucose-deprived conditions. Its mode of action was proposed to involve inhibition of increased GRP78 (glucose regulated protein 78) expression during hypoglycemia. Because GRP78 plays an important role in tumorigenesis, inhibitors such as VCD might harbor cancer therapeutic potential. We therefore sought to characterize VCD’s anticancer activity in vitro. Triple-negative breast cancer cell lines MDA-MB-231 and MDA-MB-468 were treated with VCD under different conditions known to trigger increased expression of GRP78, and a variety of cellular processes were analyzed. We show that VCD was highly cytotoxic only under hypoglycemic conditions, but not in the presence of normal glucose levels, and VCD blocked GRP78 expression only when glycolysis was impaired (due to hypoglycemia or the presence of the glycolysis inhibitor 2-deoxyglucose), but not when GRP78 was induced by other means (hypoxia, thapsigargin, tunicamycin). However, VCD’s strictly hypoglycemia-specific toxicity was not due to the inhibition of GRP78. Rather, VCD blocked mitochondrial energy production via inhibition of complex I of the electron transport chain. As a result, cellular ATP levels were quickly depleted under hypoglycemic conditions, and common cellular functions, including general protein synthesis, deteriorated and resulted in cell death. Altogether, our study identifies mitochondria as the primary target of VCD. The possibility that other purported GRP78 inhibitors (arctigenin, biguanides, deoxyverrucosidin, efrapeptin, JBIR, piericidin, prunustatin, pyrvinium, rottlerin, valinomycin, versipelostatin) might act in a similar GRP78-independent fashion will be discussed. PMID:23755268

  12. Repositioning of Verrucosidin, a purported inhibitor of chaperone protein GRP78, as an inhibitor of mitochondrial electron transport chain complex I.

    Directory of Open Access Journals (Sweden)

    Simmy Thomas

    Full Text Available Verrucosidin (VCD belongs to a group of fungal metabolites that were identified in screening programs to detect molecules that preferentially kill cancer cells under glucose-deprived conditions. Its mode of action was proposed to involve inhibition of increased GRP78 (glucose regulated protein 78 expression during hypoglycemia. Because GRP78 plays an important role in tumorigenesis, inhibitors such as VCD might harbor cancer therapeutic potential. We therefore sought to characterize VCD's anticancer activity in vitro. Triple-negative breast cancer cell lines MDA-MB-231 and MDA-MB-468 were treated with VCD under different conditions known to trigger increased expression of GRP78, and a variety of cellular processes were analyzed. We show that VCD was highly cytotoxic only under hypoglycemic conditions, but not in the presence of normal glucose levels, and VCD blocked GRP78 expression only when glycolysis was impaired (due to hypoglycemia or the presence of the glycolysis inhibitor 2-deoxyglucose, but not when GRP78 was induced by other means (hypoxia, thapsigargin, tunicamycin. However, VCD's strictly hypoglycemia-specific toxicity was not due to the inhibition of GRP78. Rather, VCD blocked mitochondrial energy production via inhibition of complex I of the electron transport chain. As a result, cellular ATP levels were quickly depleted under hypoglycemic conditions, and common cellular functions, including general protein synthesis, deteriorated and resulted in cell death. Altogether, our study identifies mitochondria as the primary target of VCD. The possibility that other purported GRP78 inhibitors (arctigenin, biguanides, deoxyverrucosidin, efrapeptin, JBIR, piericidin, prunustatin, pyrvinium, rottlerin, valinomycin, versipelostatin might act in a similar GRP78-independent fashion will be discussed.

  13. AR-12 suppresses dengue virus replication by down-regulation of PI3K/AKT and GRP78.

    Science.gov (United States)

    Chen, Hsin-Hsin; Chen, Chien-Chin; Lin, Yee-Shin; Chang, Po-Chun; Lu, Zi-Yi; Lin, Chiou-Feng; Chen, Chia-Ling; Chang, Chih-Peng

    2017-06-01

    Dengue virus (DENV) infection has become a public health issue of worldwide concern and is a serious health problem in Taiwan, yet there are no approved effective antiviral drugs to treat DENV. The replication of DENV requires both viral and cellular factors. Targeting host factors may provide a potential antiviral strategy. It has been known that up-regulation of PI3K/AKT signaling and GRP78 by DENV infection supports its replication. AR-12, a celecoxib derivative with no inhibiting activity on cyclooxygenase, shows potent inhibitory activities on both PI3K/AKT signaling and GRP78 expression levels, and recently has been found to block the replication of several hemorrhagic fever viruses. However the efficacy of AR-12 in treating DENV infection is still unclear. Here, we provide evidence to show that AR-12 is able to suppress DENV replication before or after virus infection in cell culture and mice. The antiviral activities of AR-12 are positive against infection of the four different DENV serotypes. AR-12 significantly down-regulates the PI3K/AKT activity and GRP78 expression in DENV infected cells whereas AKT and GRP78 rescue are able to attenuate anti-DENV effect of AR-12. Using a DENV-infected suckling mice model, we further demonstrate that treatment of AR-12 before or after DENV infection reduces virus replication and mice mortality. In conclusion, we uncover the potential efficacy of AR-12 as a novel drug for treating dengue. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Converging Wages, Diverging GRP: Directed Technical Change and Endogenous Growth. Empirical Analysis of Growth Patterns across Kazakh regions

    OpenAIRE

    Alisher Aldashev

    2011-01-01

    The paper analyzes unequal regional development in Kazakhstan. Applying the nonlinear least squares method in presence of spatial correlation we estimate the convergence rate of wages across Kazakh regions for the period 2003-2009. The estimated convergence rate is about 3% which is somewhat higher than estimates obtained for the USA and Europe. At the same time there is slight divergence in the GRP per capita. It is argued that convergence in wages which coincides with divergence in the per ...

  15. Hypoxic Preconditioning Promotes the Bioactivities of Mesenchymal Stem Cells via the HIF-1?-GRP78-Akt Axis

    OpenAIRE

    Lee, Jun Hee; Yoon, Yeo Min; Lee, Sang Hun

    2017-01-01

    Mesenchymal stem cells (MSC) are ideal materials for stem cell-based therapy. As MSCs reside in hypoxic microenvironments (low oxygen tension of 1% to 7%), several studies have focused on the beneficial effects of hypoxic preconditioning on MSC survival; however, the mechanisms underlying such effects remain unclear. This study aimed to uncover the potential mechanism involving 78-kDa glucose-regulated protein (GRP78) to explain the enhanced MSC bioactivity and survival in hindlimb ischemia. ...

  16. ;Prog' rarti'me"

    African Journals Online (AJOL)

    Endorsed by every country of the world, the Global AIDS ... work is not intended to control the activities of NGOs-simply to ... The balance between cooperation and maintaining indepen- ... future illness and death: life choices about sexuality, marriage or ... the United States of America and many European countries. In.

  17. Binding to membrane proteins within the endoplasmic reticulum cannot explain the retention of the glucose-regulated protein GRP78 in Xenopus oocytes.

    Science.gov (United States)

    Ceriotti, A; Colman, A

    1988-03-01

    We have studied the compartmentation and movement of the rat 78-kd glucose-regulated protein (GRP78) and other secretory and membrane proteins in Xenopus oocytes. Full length GRP78, normally found in the lumen of rat endoplasmic reticulum (ER), is localized to a membraneous compartment in oocytes and is not secreted. A truncated GRP78 lacking the C-terminal (KDEL) ER retention signal is secreted, although at a slow rate. When the synthesis of radioactive GRP78 is confined to a polar (animal or vegetal) region of the oocyte and the subsequent movement across the oocyte monitored, we find that both full-length and truncated GRP78 move at similar rates and only slightly slower than a secretory protein, chick ovalbumin. In contrast, a plasma membrane protein (influenza haemagglutinin) and two ER membrane proteins (rotavirus VP10 and a mutant haemagglutinin) remained confined to their site of synthesis. We conclude that the retention of GRP78 in the ER is not due to its tight binding to a membrane-bound receptor.

  18. Avian reovirus S1133-induced apoptosis is associated with Bip/GRP79-mediated Bim translocation to the endoplasmic reticulum.

    Science.gov (United States)

    Lin, Ping-Yuan; Liu, Hung-Jen; Chang, Ching-Dong; Chen, Yo-Chia; Chang, Chi-I; Shih, Wen-Ling

    2015-04-01

    In this study the mechanism of avian reovirus (ARV) S1133-induced pathogenesis was investigated, with a focus on the contribution of ER stress to apoptosis. Our results showed that upregulation of the ER stress response protein, as well as caspase-3 activation, occurred in ARV S1133-infected cultured cells and in SPF White Leghorn chicks organs. Upon infection, Bim was translocated specifically to the ER, but not mitochondria, in the middle to late infectious stages. In addition, ARV S1133 induced JNK phosphorylation and promoted JNK-Bim complex formation, which correlated with the Bim translocation and apoptosis induction that was observed at the same time point. Knockdown of BiP/GRP78 by siRNA and inhibition of BiP/GRP78 using EGCG both abolished the formation of the JNK-Bim complex, caspase-3 activation, and subsequent apoptosis induction by ARV S1133 efficiently. These results suggest that BiP/GRP78 played critical roles and works upstream of JNK-Bim in response to the ARV S1133-mediated apoptosis process.

  19. Inhibition of casein kinase 2 modulates XBP1-GRP78 arm of unfolded protein responses in cultured glial cells.

    Directory of Open Access Journals (Sweden)

    Toru Hosoi

    Full Text Available Stress signals cause abnormal proteins to accumulate in the endoplasmic reticulum (ER. Such stress is known as ER stress, which has been suggested to be involved in neurodegenerative diseases, diabetes, obesity and cancer. ER stress activates the unfolded protein response (UPR to reduce levels of abnormal proteins by inducing the production of chaperon proteins such as GRP78, and to attenuate translation through the phosphorylation of eIF2α. However, excessive stress leads to apoptosis by generating transcription factors such as CHOP. Casein kinase 2 (CK2 is a serine/threonine kinase involved in regulating neoplasia, cell survival and viral infections. In the present study, we investigated a possible linkage between CK2 and ER stress using mouse primary cultured glial cells. 4,5,6,7-tetrabromobenzotriazole (TBB, a CK2-specific inhibitor, attenuated ER stress-induced XBP-1 splicing and subsequent induction of GRP78 expression, but was ineffective against ER stress-induced eIF2α phosphorylation and CHOP expression. Similar results were obtained when endogenous CK2 expression was knocked-down by siRNA. Immunohistochemical analysis suggested that CK2 was present at the ER. These results indicate CK2 to be linked with UPR and to resist ER stress by activating the XBP-1-GRP78 arm of UPR.

  20. Eficiência da seleção de progênies de café F4 pela metodologia de modelos mistos (REML/BLUP

    Directory of Open Access Journals (Sweden)

    Thamiris Bandoni Pereira

    2013-01-01

    Full Text Available Objetivou-se verificar a eficiência da seleção de cafeeiros em geração F4 para características agronômicas e resistência a doenças mediante o emprego da metodologia de modelos mistos de Henderson. O experimento foi instalado na Fazenda Experimental da EPAMIG, em Machado, MG, sendo utilizadas progênies F4 derivadas do cruzamento entre cultivares do grupo Icatu com Catuaí Amarelo IAC 62 e IAC 17 e com as progênies IAC 5002 e IAC 5010. O delineamento experimental utilizado foi o látice com quatro repetições e as avaliações foram realizadas ao longo de dois anos (2010 e 2011. Foram avaliadas as seguintes características: produção (litros planta-1, reação à ferrugem, vigor vegetativo e reação à cercosporiose. Foram utilizados o procedimento REML/BLUP para estimar os parâmetros genéticos, o índice de seleção de Mulamba e Mock a fim de verificar os ganhos para múltiplos caracteres, a acurácia e eficiência, calculadas a partir do diferente número de medições e, ainda, o agrupamento multivariado de progênies pelo método de Tocher. Verificou-se, a partir da análise dos parâmetros genéticos, um maior sucesso na seleção entre famílias para todas as características avaliadas. A adoção de quatro colheitas poderá elevar a acurácia a 80% na seleção de indivíduos para as características produção, reação à ferrugem e vigor vegetativo. A seleção das 12 melhores progênies para caracteres múltiplos pelo índice de Mulamba e Mock proporcionaria um ganho médio de 70,4%.

  1. Biokinetics and dosimetry in patients of {sup 99m}Tc-HYNIC-Lys{sup 3}-Bombesin: images of GRP receptors; Biocinetica y dosimetria en humanos de {sup 99m}Tc-HYNIC-Lys{sup 3}-Bombesina: imagenes de receptores GRP

    Energy Technology Data Exchange (ETDEWEB)

    Santos C, C L [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    2007-07-01

    The bombesin (BN) receptor subtype 2 (GRP-r) is expressed in several normal human tissues and is over-expressed in various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently [{sup 99m}Tc]EDDA/HYNIC-Lys{sup 3}-bombesin ({sup 99m}Tc-HYNIC-BN) was reported as a radiopharmaceutical with high stability in human serum, specific cell GRP-r binding and rapid cell internalization. The aim of this study was to evaluate the feasibility of using {sup 99m}Tc-HYNIC-BN to image GRP-r and to assess the radiopharmaceutical biokinetics and dosimetry in 4 breast cancer patients and in 7 healthy women. Methods: Whole-body images were acquired at 20, 90, 180 min and 24 h after {sup 99m}Tc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source organs on each time frame. The same set of ROIs was used for all 11 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-HYNIC-BN time-activity curves in each organ in order to calculate the total number of disintegrations (N) that occurred in the source regions, according with MIRD methodology. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Results: Images showed a rapid radiopharmaceutical blood clearance with renal excretion as predominant route. {sup 99m}Tc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in cancer mammary glands and well differentiated from the ubiquitous GRP-r expression in normal breast, lungs and airways. There was no statistically significant difference (p > 0.05) in the radiation absorbed doses between cancer patients and healthy women. The average equivalent doses (n=11) for a study using 740 MBq were 24.8 +- 8.8 mSv (kidneys), 7.3 +- 1.8 mSv (lungs), 6.5 +- 4.0 mSv (breast) 2.0 +- 0.3 mSv (pancreas), 1.6 +- 0.3 mSv (liver), 1.2 +- 0.2 mSv (ovaries) and 1.0 +- 0.2 mSv (red

  2. Administrative Circular N° 26 (Rev.  5) - November 2004

    CERN Multimedia

    Human Resources Department

    2004-01-01

    Procedure governing the career evolution of staff members The introduction of an electronic individual appraisal report form via EDH for the MAPS exercise entails some modifications to Administrative Circular N° 26 (Rev. 4). The revised version (Rev. 5) is available in departmental secretariats as well as on the Web at the following address: http://cern.ch/hr-web/internal/admin_services/admincirc/listadmincirc.asp Human Resources Department Tel. 74128

  3. Altered Sleep Homeostasis in Rev-erbα Knockout Mice.

    Science.gov (United States)

    Mang, Géraldine M; La Spada, Francesco; Emmenegger, Yann; Chappuis, Sylvie; Ripperger, Jürgen A; Albrecht, Urs; Franken, Paul

    2016-03-01

    The nuclear receptor REV-ERBα is a potent, constitutive transcriptional repressor critical for the regulation of key circadian and metabolic genes. Recently, REV-ERBα's involvement in learning, neurogenesis, mood, and dopamine turnover was demonstrated suggesting a specific role in central nervous system functioning. We have previously shown that the brain expression of several core clock genes, including Rev-erbα, is modulated by sleep loss. We here test the consequences of a loss of REV-ERBα on the homeostatic regulation of sleep. EEG/EMG signals were recorded in Rev-erbα knockout (KO) mice and their wild type (WT) littermates during baseline, sleep deprivation, and recovery. Cortical gene expression measurements after sleep deprivation were contrasted to baseline. Although baseline sleep/wake duration was remarkably similar, KO mice showed an advance of the sleep/wake distribution relative to the light-dark cycle. After sleep onset in baseline and after sleep deprivation, both EEG delta power (1-4 Hz) and sleep consolidation were reduced in KO mice indicating a slower increase of homeostatic sleep need during wakefulness. This slower increase might relate to the smaller increase in theta and gamma power observed in the waking EEG prior to sleep onset under both conditions. Indeed, the increased theta activity during wakefulness predicted delta power in subsequent NREM sleep. Lack of Rev-erbα increased Bmal1, Npas2, Clock, and Fabp7 expression, confirming the direct regulation of these genes by REV-ERBα also in the brain. Our results add further proof to the notion that clock genes are involved in sleep homeostasis. Because accumulating evidence directly links REV-ERBα to dopamine signaling the altered homeostatic regulation of sleep reported here are discussed in that context. © 2016 Associated Professional Sleep Societies, LLC.

  4. Biokinetics and dosimetry of 99m Tc-EDDA/HYNIC-[Lys3]-bombesin in humans: imaging of GRP receptors

    International Nuclear Information System (INIS)

    Santos C, C.L.; Ferro F, G.; Murphy, C.A de; Cardena, E.; Pichardo R, P.

    2007-01-01

    Full text: Bombesin (BN) receptor subtype 2 (GRP-r) is over-expressed on various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently we reported the 99- mTc-EDDA/HYNIC-[Lys 3 ]-Bombesin ( 99m Tc-HYNIC-BN) complex as a new radiopharmaceutical with high stability in human serum, specific cell GRP-receptor binding and rapid internalization. The aim of this study was to evaluate the 99m Tc-HYNIC-BN biokinetics and dosimetry in 5-healthy and 3-breast cancer women. Whole-body images were acquired at 20, 90, 180 min and 24 h after 99m Tc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source' organs on each time frame. The same set of ROIs was used for all 8 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate 99m Tc-HYNIC-BN time activity curves in each organ, to calculate the total number of disintegrations (N) that occurred in the source regions. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Images showed a rapid radiopharmaceutical blood clearance with predominantly renal excretion and minimal hepatobiliary elimination. 99m Tc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in breast cancer lesions and well differentiated from GRP-r expression in lungs and airways with normal GRP-r density (ratio 3:1). The equivalent doses for a study using 370 MBq were 7.38±1.68, 0.59±0.08, 2.07±0.60, 0.58±0.1, 0.75±0.09 and 0.43±0.07 mSv for kidneys, liver, lungs, ovaries, pancreas and red marrow respectively. The effective dose was 1.64±0.25 mSv which is comparable with the doses known for most of the 99m Tc radiopharmaceutical studies in nuclear medicine. (Author)

  5. Biokinetics and dosimetry in patients of 99mTc-HYNIC-Lys3-Bombesin: images of GRP receptors

    International Nuclear Information System (INIS)

    Santos C, C. L.

    2007-01-01

    The bombesin (BN) receptor subtype 2 (GRP-r) is expressed in several normal human tissues and is over-expressed in various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently [ 99m Tc]EDDA/HYNIC-Lys 3 -bombesin ( 99m Tc-HYNIC-BN) was reported as a radiopharmaceutical with high stability in human serum, specific cell GRP-r binding and rapid cell internalization. The aim of this study was to evaluate the feasibility of using 99m Tc-HYNIC-BN to image GRP-r and to assess the radiopharmaceutical biokinetics and dosimetry in 4 breast cancer patients and in 7 healthy women. Methods: Whole-body images were acquired at 20, 90, 180 min and 24 h after 99m Tc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source organs on each time frame. The same set of ROIs was used for all 11 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate 99m Tc-HYNIC-BN time-activity curves in each organ in order to calculate the total number of disintegrations (N) that occurred in the source regions, according with MIRD methodology. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Results: Images showed a rapid radiopharmaceutical blood clearance with renal excretion as predominant route. 99m Tc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in cancer mammary glands and well differentiated from the ubiquitous GRP-r expression in normal breast, lungs and airways. There was no statistically significant difference (p > 0.05) in the radiation absorbed doses between cancer patients and healthy women. The average equivalent doses (n=11) for a study using 740 MBq were 24.8 +- 8.8 mSv (kidneys), 7.3 +- 1.8 mSv (lungs), 6.5 +- 4.0 mSv (breast) 2.0 +- 0.3 mSv (pancreas), 1.6 +- 0.3 mSv (liver), 1.2 +- 0.2 mSv (ovaries) and 1.0 +- 0.2 mSv (red marrow). The mean effective dose

  6. POSSIBILITIES OF THE USE OF GRP PIPING IN THE CONSTRUCTION AND RECONSTRUCTION OF ENGINEERING NETWORKS

    Directory of Open Access Journals (Sweden)

    ikitina Irina Nikolaevna

    2015-12-01

    Full Text Available Today in modern construction new technologies and materials are used for the manufacture of pipelines for water supply and sanitation. They are supposed to operate for at least 50 years. Unlike plastic pipes, fiberglass ones may be made of larger sizes — up to 3700 mm in diameter. They are produced using the technology of optical fiber winding, which is carried out according to modern international standards of quality. The basic raw materials — fiberglass and resin — are produced in Russia, but their production is limited, so they are purchased abroad, which increases the cost of manufacture of this type of piping. However, due to the necessity of laying pipelines of large diameter, which cannot be made with plastic pipes, the manufacture of GRP pipes will increase. The experience of laying and constructing this type of pipelines, for example, in the areas of hot water supply allows concluding that they are able to withstand the temperatures of up to 150 °C, while their weight is four times less than the weight of steel pipes (they are easily installed with the help of small lifting equipment and by a team of six people. It should be noted that the use of fiberglass pipes helps to reduce the costs of system operation, because this type of piping is not subject to corrosion and encrustation of the inner surface, since it has a low level of roughness, which, for example, is 0.013 for a steel pipe, and 0.01 for fiberglass pipe. Thus, it is not necessary to put protective corrosion-resistant coatings and to provide an expensive protection against electrochemical corrosion. Piping made of fiberglass pipes can be designed as underground, above-ground with stacking or raised on poles. It is possible to combine these options.

  7. The 78 kDa glucose-regulated protein (GRP78/BIP) is expressed on the cell membrane, is released into cell culture medium and is also present in human peripheral circulation.

    Science.gov (United States)

    Delpino, Andrea; Castelli, Mauro

    2002-01-01

    In human rabdomiosarcoma cells (TE671/RD) chronic exposure to 500 nM thapsigargin (a powerful inhibitor of the endoplasmic reticulum Ca2+-ATPases) resulted in the induction of the stress protein GRP78/BIP. Making use of the surface biotinylation method, followed by the isolation of the GRP78 using ATP-agarose affinity chromatography, it was found that a fraction of the thapsigargin-induced GRP78 is expressed on the cell surface. The presence of GRP78 on the membrane of thapsigargin-treated cells was confirmed by fractionation of cell lysates into a soluble and a membrane fraction, followed by Western blot analysis with an anti-GRP78 antibody. It was also found that conspicuous amounts of GRP78 are present in the culture medium collected from thapsigargin-treated cultures. This extracellular GRP78 originates mostly by an active release from intact cells and does not result solely from the leakage of proteins from dead cells. Moreover, small amounts of circulating, free GRP78 and naturally-occurring anti-GRP78 autoantibodies were detected in the peripheral circulation of healthy human individuals.

  8. Peripheral T-Cell Reactivity to Heat Shock Protein 70 and Its Cofactor GrpE from Tropheryma whipplei Is Reduced in Patients with Classical Whipple's Disease.

    Science.gov (United States)

    Trotta, Lucia; Weigt, Kathleen; Schinnerling, Katina; Geelhaar-Karsch, Anika; Oelkers, Gerrit; Biagi, Federico; Corazza, Gino Roberto; Allers, Kristina; Schneider, Thomas; Erben, Ulrike; Moos, Verena

    2017-08-01

    Classical Whipple's disease (CWD) is characterized by the lack of specific Th1 response toward Tropheryma whipplei in genetically predisposed individuals. The cofactor GrpE of heat shock protein 70 (Hsp70) from T. whipplei was previously identified as a B-cell antigen. We tested the capacity of Hsp70 and GrpE to elicit specific proinflammatory T-cell responses. Peripheral mononuclear cells from CWD patients and healthy donors were stimulated with T. whipplei lysate or recombinant GrpE or Hsp70 before levels of CD40L, CD69, perforin, granzyme B, CD107a, and gamma interferon (IFN-γ) were determined in T cells by flow cytometry. Upon stimulation with total bacterial lysate or recombinant GrpE or Hsp70 of T. whipplei , the proportions of activated effector CD4 + T cells, determined as CD40L + IFN-γ + , were significantly lower in patients with CWD than in healthy controls; CD8 + T cells of untreated CWD patients revealed an enhanced activation toward unspecific stimulation and T. whipplei -specific degranulation, although CD69 + IFN-γ + CD8 + T cells were reduced upon stimulation with T. whipplei lysate and recombinant T. whipplei -derived proteins. Hsp70 and its cofactor GrpE are immunogenic in healthy individuals, eliciting effective responses against T. whipplei to control bacterial spreading. The lack of specific T-cell responses against these T. whipplei -derived proteins may contribute to the pathogenesis of CWD. Copyright © 2017 American Society for Microbiology.

  9. Binding and thermodynamics of REV peptide-ctDNA interaction.

    Science.gov (United States)

    Upadhyay, Santosh Kumar

    2017-03-01

    The thermodynamics of DNA-ligand binding is important as it provides useful information to understand the details of binding processes. HIV-1 REV response element (RRE) located in the env coding region of the viral genome is reported to be well conserved across different HIV-1 isolates. In this study, the binding characteristics of Calf thymus DNA (ctDNA) and REV peptide from HIV-1 were investigated using spectroscopic (UV-visible, fluorescence, and circular dichroism (CD)) and isothermal titration calorimetric (ITC) techniques. Thermal stability and ligand binding properties of the ctDNA revealed that native ctDNA had a T m of 75.5 °C, whereas the ctDNA-REV peptide complex exhibited an incremental shift in the T m by 8 °C, indicating thermal stability of the complex. CD data indicated increased ellipticity due to large conformational changes in ctDNA molecule upon binding with REV peptide and two binding stoichiometric modes are apparent. The ctDNA experienced condensation due to large conformational changes in the presence of REV peptide and positive B→Ψ transition was observed at higher molar charge ratios. Fluorescence studies performed at several ligand concentrations revealed a gradual decrease in the fluorescence intensity of EtBr-bound ctDNA in response to increasing ligand concentrations. The fluorescence data further confirmed two stoichiometric modes of binding for ctDNA-REV peptide complex as previously observed with CD studies. The binding enthalpies were determined using ITC in the temperature range of 293 K-308 K. The ITC binding isotherm was exothermic at all temperatures examined, with low ΔH values indicating that the ctDNA-REV peptide interaction is driven largely by entropy. The heat capacity change (ΔC p ) was insignificant, an unusual finding in the area of DNA-peptide interaction studies. The variation in the values obtained for ΔH, ΔS, and ΔG with temperature further suggests that ctDNA-REV peptide interaction is entropically

  10. Field survey and laboratory tests on composite materials case of GRP (Glass Fiber Reinforced Polyester tubes for water suply

    Directory of Open Access Journals (Sweden)

    Radu Hariga

    2013-09-01

    Full Text Available In the Moldova land, were made two lines of water adduction, having 6000 m length and 40 m slope, or 1/150 slope. The water supply component tubes were disposed under the plant: The tubes are made of glass – reinforced thermosetting plastics (GRP. After about 180 days of operation, one of the lines showed severe deterioration of the quality pipe components. This paper deals with some laboratory tests in order to detect the failure cases of the pipelines components.

  11. Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel insights into the regulation of Rev nuclear import.

    LENUS (Irish Health Repository)

    Gu, Lili

    2011-01-01

    The HIV-1 regulatory protein Rev, which is essential for viral replication, mediates the nuclear export of unspliced viral transcripts. Rev nuclear function requires active nucleocytoplasmic shuttling, and Rev nuclear import is mediated by the recognition of its Nuclear Localisation Signal (NLS) by multiple import factors, which include transportin and importin β. However, it remains unclear which nuclear import pathway(s) predominate in vivo, and the cellular environment that modulates Rev nucleocytoplasmic shuttling remains to be characterised.

  12. Administrative Circular No. 26 (Rev. 9) – Recognition of Merit

    CERN Multimedia

    2012-01-01

    Administrative Circular No. 26 (Rev. 9) entitled "Recognition of Merit”, approved by the Director-General following discussion in the Standing Concertation Committee meeting on 27 September 2011 is available on the intranet site of the Human Resources Department: https://cern.ch/hr-docs/admincirc/admincirc.asp The circular was above all revised in order to integrate the new CERN Competency Model into the annual procedure of performance appraisal. It cancels and replaces Administrative Circular No. 26 (Rev. 8) entitled "Recognition of merit” of September 2008. Department Head Office HR Department

  13. Administrative Circular No. 26 (Rev. 7) – May 2007

    CERN Multimedia

    HR Department

    2007-01-01

    Recognition of Merit of Staff Members Administrative Circular No. 26 (Rev. 7) is now available on the intranet site of the Human Resources Department. This circular cancels and replaces Administrative Circular No. 26 (Rev. 6) - Procedures governing the career development of staff members. Copies will shortly be available in Departmental secretariats. If you require any additional information on the new staff-member merit assessment and recognition system, you may consult the FAQ, which has been available on the Human Resources Department intranet site since February 2007. Human Resources Department Tel. 78003

  14. Divergência genética entre progênies de café robusta Genetic divergence among robusta coffe progenies

    Directory of Open Access Journals (Sweden)

    Milana Gonçalves Ivoglo

    2008-12-01

    Full Text Available Estudou-se a divergência genética de 21 progênies de meios-irmãos - 19 do grupo Congolês e duas do grupo Guineano - de introduções do germoplasma de café robusta (Coffea canephora do IAC. O estudo baseou-se em análises multivariadas de 14 características morfo-agronômicas, com o propósito de selecionar as progênies mais divergentes, visando à definição de população-base para posterior seleção e produção de híbridos. Avaliou-se também a importância das características discriminantes para análises de divergência, visando ao descarte das variáveis, segundo suas contribuições relativas. O experimento foi plantado e desenvolvido em campo experimental localizado no Pólo Regional do Nordeste Paulista, Mococa (SP, em blocos casualizados, com 21 tratamentos e 24 repetições. O agrupamento dos genótipos foi realizado com base nos métodos de Tocher e UPGMA. A matriz de dissimilaridade genética foi obtida por meio da distância generalizada de Mahalanobis, que serviu de base para a formação dos grupos. Os métodos empregados foram eficientes em detectar ampla variabilidade genética entre as progênies avaliadas. Vários grupos dissimilares foram identificados. As progênies IAC 2262, IAC 2290, IAC 2286, IAC 2292 e IAC 2291 são indicadas para compor programas de intercruzamentos, por terem sido consideradas as mais promissoras na obtenção de populações segregantes ou híbridos heteróticos. As características que menos contribuíram para a divergência genética foram, hierarquicamente: diâmetro da copa antes da poda, altura da planta antes da poda e área foliar.It was studied genetic divergence of 21 half-sib progenies, being 19 of the Congolês group and two of the Guineano group, introductions of germoplasma robust (Coffea canephora, based in 14 morpho-agronomic traits and multivariate procedures. It's aims to select the lineages most divergent for definition of population-base for posterior reciprocal

  15. Selective in vitro targeting of GRP and NMB receptors in human tumours with the new bombesin tracer 177Lu-AMBA

    International Nuclear Information System (INIS)

    Waser, Beatrice; Eltschinger, Veronique; Reubi, Jean C.; Linder, Karen; Nunn, Adrian

    2007-01-01

    To investigate the in vitro binding properties of a novel radiolabelled bombesin analogue, 177 Lu-AMBA, in human neoplastic and non-neoplastic tissues selected for their expression of the bombesin receptor subtypes GRP-R, NMB-R and BRS-3. In vitro receptor autoradiography was performed in cancers expressing the various bombesin receptor subtypes. The novel radioligand 177 Lu-AMBA was used and compared with established bombesin radioligands such as 125 I-Tyr 4 -bombesin and 125 I-[D-Tyr 6 ,β-Ala 11 ,Phe 13 ,Nle 14 ]-bombesin(6-14). In vitro incidence of detection of each of the three bombesin receptor subtypes was evaluated in each tumour. 177 Lu-AMBA identified all GRP-R-expressing tumours, such as prostatic, mammary and renal cell carcinomas as well as gastrointestinal stromal tumours. 177 Lu-AMBA also identified all NMB-expressing tumours, but did not detect BRS-3-expressing tumours or BRS-3-expressing pancreatic islets. GRP-R-expressing peritumoural vessels were heavily labelled with 177 Lu-AMBA. In contrast to the strongly GRP-R-positive mouse pancreas, the human pancreas was not labelled with 177 Lu-AMBA unless chronic pancreatitis was diagnosed. In general, the sensitivity was slightly better with 177 Lu-AMBA than with the conventional bombesin radioligands. The present in vitro study suggests that 177 Lu-AMBA may be a very useful in vivo targeting agent for GRP-R-expressing tumours, NMB-R-expressing tumours and GRP-R-expressing neoangiogenic vessels. (orig.)

  16. Selective in vitro targeting of GRP and NMB receptors in human tumours with the new bombesin tracer {sup 177}Lu-AMBA

    Energy Technology Data Exchange (ETDEWEB)

    Waser, Beatrice; Eltschinger, Veronique; Reubi, Jean C. [University of Berne, Division of Cell Biology and Experimental Cancer Research, Institute of Pathology, P.O. Box 62, Bern (Switzerland); Linder, Karen; Nunn, Adrian [Bracco Research USA Inc, Princeton, NJ (United States)

    2007-01-15

    To investigate the in vitro binding properties of a novel radiolabelled bombesin analogue, {sup 177}Lu-AMBA, in human neoplastic and non-neoplastic tissues selected for their expression of the bombesin receptor subtypes GRP-R, NMB-R and BRS-3. In vitro receptor autoradiography was performed in cancers expressing the various bombesin receptor subtypes. The novel radioligand {sup 177}Lu-AMBA was used and compared with established bombesin radioligands such as {sup 125}I-Tyr{sup 4}-bombesin and {sup 125}I-[D-Tyr{sup 6},{beta}-Ala{sup 11},Phe{sup 13},Nle{sup 14}]-bombesin(6-14). In vitro incidence of detection of each of the three bombesin receptor subtypes was evaluated in each tumour. {sup 177}Lu-AMBA identified all GRP-R-expressing tumours, such as prostatic, mammary and renal cell carcinomas as well as gastrointestinal stromal tumours. {sup 177}Lu-AMBA also identified all NMB-expressing tumours, but did not detect BRS-3-expressing tumours or BRS-3-expressing pancreatic islets. GRP-R-expressing peritumoural vessels were heavily labelled with {sup 177}Lu-AMBA. In contrast to the strongly GRP-R-positive mouse pancreas, the human pancreas was not labelled with {sup 177}Lu-AMBA unless chronic pancreatitis was diagnosed. In general, the sensitivity was slightly better with {sup 177}Lu-AMBA than with the conventional bombesin radioligands. The present in vitro study suggests that {sup 177}Lu-AMBA may be a very useful in vivo targeting agent for GRP-R-expressing tumours, NMB-R-expressing tumours and GRP-R-expressing neoangiogenic vessels. (orig.)

  17. Clinical value of Pro-GRP and T lymphocyte subpopulation for the assessment of immune functions of lung cancer patients after DC-CIK biological therapy.

    Science.gov (United States)

    He, Lijie; Wang, Jing; Chang, Dandan; Lv, Dandan; Li, Haina; Zhang, Heping

    2018-02-01

    The present study investigated the aptness of assessing the levels of progastrin-releasing peptide (Pro-GRP) in addition to the T lymphocyte subpopulation in lung cancer patients prior to and after therapy for determining immune function. A total of 45 patients with lung cancer were recruited and stratified in to a non-small cell lung cancer (NSCLC) and an SCLC group. Prior to and after treatment by combined biological therapy comprising chemotherapy or chemoradiotherapy followed by three cycles of retransformation of autologous dendritic cells-cytokine-induced killer cells (DC-CIK), the peripheral blood was assessed for populations of CD3 + , CD4 + , CD8 + and regulatory T cells (Treg) by flow cytometry, and for the levels of pro-GRP, carcinoembryonic antigen, neuron-specific enolase and Cyfra 21-1. The results revealed that in NSCLC patients, CD8 + T lymphocytes and Treg populations were decreased, and that CD3 + and CD4 + T lymphocytes as well as the CD4 + /CD8 + ratio were increased after therapy; in SCLC patients, CD3 + , CD4 + and CD8 + T lymphocytes were increased, while Treg cells were decreased after treatment compared with those at baseline. In each group, Pro-GRP was decreased compared with that prior to treatment, and in the SCLC group only, an obvious negative correlation was identified between Pro-GRP and the T lymphocyte subpopulation. Furthermore, a significant correlation between Pro-GRP and Tregs was identified in each group. In conclusion, the present study revealed that the immune function of the patients was improved after biological therapy. The results suggested a significant correlation between Pro-GRP and the T lymphocyte subpopulation in SCLC patients. Detection of Pro-GRP may assist the early clinical diagnosis of SCLC and may also be used to assess the immune regulatory function of patients along with the T lymphocyte subpopulation. Biological therapy with retransformed autologous DC-CIK was indicated to enhance the specific elimination

  18. RNAi silenced Dd-grp94 (Dictyostelium discoideum glucose-regulated protein 94 kDa) cell lines in Dictyostelium exhibit marked reduction in growth rate and delay in development.

    Science.gov (United States)

    Baviskar, Sandhya N; Shields, Malcolm S

    2010-01-01

    Glucose-regulated 94 kDa protein (Grp94) is a resident of the endoplasmic reticulum (ER) of multicellular eukaryotes. It is a constitutively expressed protein that is overexpressed in certain abnormal conditions of the cell such as depletion of glucose and calcium, and low oxygen and pH. The protein is also implicated in diseased conditions like cancer and Alzheimer's disease. In this study, the consequences of downregulation of Grp94 were investigated at both unicellular and multicellular stages of Dictyostelium discoideum. Previous studies have shown the expression of Dd-Grp94 (Dictyostelium discoideum glucose-regulated 94 kDa protein) in wild-type cells varies during development, and overexpression of Dd-Grp94 leads to abnormal cell shape and inhibition of development (i.e., formation of fruiting bodies). Grp94 is a known calcium binding protein and an efficient calcium buffer. Therefore, in the present study we hypothesized that downregulation of Dd-Grp94 protein would affect Dictyostelium cell structure, growth, and development. We found that Dd-grp94 RNAi recombinants exhibited reduced growth rate, cell size, and a subtle change in cell motility compared to the parental cells. The recombinants also exhibited a delay in development and small fruiting bodies. These results establish that Dd-grp94 plays a crucial role in determining normal cell structure, growth and differentiation.

  19. Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of Rev nuclear import.

    LENUS (Irish Health Repository)

    Gu, Lili

    2011-03-14

    Abstract Background The HIV-1 regulatory protein Rev, which is essential for viral replication, mediates the nuclear export of unspliced viral transcripts. Rev nuclear function requires active nucleocytoplasmic shuttling, and Rev nuclear import is mediated by the recognition of its Nuclear Localisation Signal (NLS) by multiple import factors, which include transportin and importin β. However, it remains unclear which nuclear import pathway(s) predominate in vivo, and the cellular environment that modulates Rev nucleocytoplasmic shuttling remains to be characterised. Results In our study, we have identified the cellular protein HIC (Human I-mfa domain-Containing protein) as a novel interactor of HIV-1 Rev. We demonstrate that HIC selectively interferes with Rev NLS interaction with importin β and impedes its nuclear import and function, but does not affect Rev nuclear import mediated by transportin. Hence, the molecular determinants mediating Rev-NLS recognition by importin β and transportin appear to be distinct. Furthermore, we have employed HIC and M9 M, a peptide specifically designed to inhibit the transportin-mediated nuclear import pathway, to characterise Rev nuclear import pathways within different cellular environments. Remarkably, we could show that in 293T, HeLa, COS7, Jurkat, U937, THP-1 and CEM cells, Rev nuclear import is cell type specific and alternatively mediated by transportin or importin β, in a mutually exclusive fashion. Conclusions Rev cytoplasmic sequestration by HIC may represent a novel mechanism for the control of Rev function. These studies highlight that the multivalent nature of the Rev NLS for different import receptors enables Rev to adapt its nuclear trafficking strategy.

  20. Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of Rev nuclear import

    Directory of Open Access Journals (Sweden)

    Sheehy Noreen

    2011-03-01

    Full Text Available Abstract Background The HIV-1 regulatory protein Rev, which is essential for viral replication, mediates the nuclear export of unspliced viral transcripts. Rev nuclear function requires active nucleocytoplasmic shuttling, and Rev nuclear import is mediated by the recognition of its Nuclear Localisation Signal (NLS by multiple import factors, which include transportin and importin β. However, it remains unclear which nuclear import pathway(s predominate in vivo, and the cellular environment that modulates Rev nucleocytoplasmic shuttling remains to be characterised. Results In our study, we have identified the cellular protein HIC (Human I-mfa domain-Containing protein as a novel interactor of HIV-1 Rev. We demonstrate that HIC selectively interferes with Rev NLS interaction with importin β and impedes its nuclear import and function, but does not affect Rev nuclear import mediated by transportin. Hence, the molecular determinants mediating Rev-NLS recognition by importin β and transportin appear to be distinct. Furthermore, we have employed HIC and M9 M, a peptide specifically designed to inhibit the transportin-mediated nuclear import pathway, to characterise Rev nuclear import pathways within different cellular environments. Remarkably, we could show that in 293T, HeLa, COS7, Jurkat, U937, THP-1 and CEM cells, Rev nuclear import is cell type specific and alternatively mediated by transportin or importin β, in a mutually exclusive fashion. Conclusions Rev cytoplasmic sequestration by HIC may represent a novel mechanism for the control of Rev function. These studies highlight that the multivalent nature of the Rev NLS for different import receptors enables Rev to adapt its nuclear trafficking strategy.

  1. A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of Rev

    International Nuclear Information System (INIS)

    Fang Jianhua; Kubota, Satoshi; Yang Bin; Zhou Naiming; Zhang Hui; Godbout, Roseline; Pomerantz, Roger J.

    2004-01-01

    HIV-1 Rev escorts unspliced viral mRNAs out of the nucleus of infected cells, which allows formation of infectious HIV-1 virions. We have identified a putative DEAD box (Asp-Glu-Ala-Asp) RNA helicase, DDX1, as a cellular co-factor of Rev, through yeast and mammalian two-hybrid systems using the N-terminal motif of Rev as 'bait'. DDX1 is not a functional homolog of HIV-1 Rev, but down-regulation of DDX1 resulted in an alternative splicing pattern of Rev-responsive element (RRE)-containing mRNA, and attenuation of Gag p24 antigen production from HLfb rev(-) cells rescued by exogenous Rev. Co-transfection of a DDX1 expression vector with HIV-1 significantly increased viral production. DDX1 binding to Rev, as well as to the RRE, strongly suggest that DDX1 affects Rev function through the Rev-RRE axis. Moreover, down-regulation of DDX1 altered the steady state subcellular distribution of Rev, from nuclear/nucleolar to cytoplasmic dominance. These findings indicate that DDX1 is a critical cellular co-factor for Rev function, which maintains the proper subcellular distribution of this lentiviral regulatory protein. Therefore, alterations in DDX1-Rev interactions could induce HIV-1 persistence and targeting DDX1 may lead to rationally designed and novel anti-HIV-1 strategies and therapeutics

  2. Operational circular No. 1 (Rev. 1) – Operational circulars

    CERN Multimedia

    HR Department

    2011-01-01

    Operational Circular No. 1 (Rev. 1) is applicable to members of the personnel and other persons concerned. Operational Circular No. 1 (Rev. 1) entitled "Operational circulars", approved following discussion at the Standing Concertation Committee meeting on 4 May 2011, is available on the intranet site of the Human Resources Department: https://hr-docs.web.cern.ch/hr-docs/opcirc/opcirc.asp It cancels and replaces Operational Circular No. 1 entitled "Operational Circulars” of December 1996. This new version clarifies, in particular, that operational circulars do not necessarily arise from the Staff Rules and Regulations, and the functional titles have been updated to bring them into line with the current CERN organigram. Department Head Office  

  3. Administrative Circular No. 26 (Rev.10) - Recognition of merit

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 26 (Rev. 10) entitled “Recognition of Merit”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 5 December 2013 and entering into force on 1 January 2014, is available on the intranet site of the Human Resources Department (see here).   This circular is applicable to staff members. It cancels and replaces Administrative Circular No. 26 (Rev. 9) entitled “Recognition of Merit” of December 2011. The circular was revised in order to take into account the work performed in the framework of an elective mandate during the exercise of merit recognition of staff members. In addition, the circular was revised to provide that, in the case of staff members on special leave for professional reasons for a period equal to or longer than half a year, it will no longer be possible to grant an exceptional advancement. Department Head Office HR Department

  4. Administrative Circular No. 23 (Rev. 4) - Special working hours

    CERN Document Server

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 23 (Rev. 4) entitled "Special working hours", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 22 March 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208539.   This revised circular cancels and replaces Administrative Circular No. 23 (Rev. 3) also entitled "Special working hours" of January 2013. This document contains modifications to reflect the new career structure and ensuring the provision consistent with practice that compensation or remuneration of special working hours performed remotely is possible only in case of emergency.   This circular will enter into force on 1st September 2016.

  5. Meteorological explanation of wake clouds at Horns Rev wind farm

    Energy Technology Data Exchange (ETDEWEB)

    Emeis, S. [Karlsruhe Institute of Technology (Germany). Inst. for Meteorology and Climate Research

    2010-08-15

    The occurrence of wake clouds at Horns Rev wind farm is explained as mixing fog. Mixing fog forms when two nearly saturated air masses with different temperature are mixed. Due to the non-linearity of the dependence of the saturation water vapour pressure on temperature, the mixed air mass is over-saturated and condensation sets in. On the day in February 2008, when the wake clouds were observed at Horns Rev, cold and very humid air was advected from the nearby land over the warmer North Sea and led to the formation of a shallow layer with sea smoke or fog close above the sea surface. The turbines mixed a much deeper layer and thus provoked the formation of cloud trails in the wakes of the turbines. (orig.)

  6. Continuous high expression of XBP1 and GRP78 is important for the survival of bone marrow cells in CCl4-treated cirrhotic liver

    International Nuclear Information System (INIS)

    Marumoto, Yoshio; Terai, Shuji; Urata, Yohei; Matsumoto, Toshihiko; Mizunaga, Yuko; Yamamoto, Naoki; Jin, Haiyan; Fujisawa, Koichi; Murata, Tomoaki; Shinoda, Koh; Nishina, Hiroshi; Sakaida, Isao

    2008-01-01

    We have previously shown that infusion of bone marrow cells (BMC) improves CCl 4 -induced cirrhosis. However, it is unclear why the injected BMC are resistant to CCl 4 damage and subsequently improve the local microenvironment in damaged liver. To analyze the cellular phenomena involved in this process, we studied the damaged liver using electron microscopy. We found that CCl 4 caused rough endoplasmic reticula to swell in hepatocytes. To analyze the gene expression patterns associated with this process, we conducted PCR-selected suppressive subtractive hybridization. We found that expression levels of HSP84, HSP40, and XBP1 differed markedly between control liver and liver infused with BMC. Immunohistochemical staining revealed that expression levels of HSP84 and HSP40 were markedly higher in the early phase of differentiation immediately after BMC infusion, but decreased over time. XBP1 expression remained high during the late phase, and GRP78 expression increased with XBP1 activation. We also found that GFP-positive BMC expressed XBP1 and GRP78. XBP1 and GRP78 are associated with ER stress. Thus, continuous high XBP1 and GRP78 expression might be essential for the survival and proliferation of BMC in a CCl 4 -induced persistent liver damage environment

  7. REV-ERB and ROR: therapeutic targets for treating myopathies

    Science.gov (United States)

    Welch, Ryan D.; Flaveny, Colin A.

    2017-08-01

    Muscle is primarily known for its mechanical roles in locomotion, maintenance of posture, and regulation of cardiac and respiratory function. There are numerous medical conditions that adversely affect muscle, myopathies that disrupt muscle development, regeneration and protein turnover to detrimental effect. Skeletal muscle is also a vital secretory organ that regulates thermogenesis, inflammatory signaling and directs context specific global metabolic changes in energy substrate preference on a daily basis. Myopathies differ in the causative factors that drive them but share common features including severe reduction in quality of life and significantly increased mortality all due irrefutably to the loss of muscle mass. Thus far clinically viable approaches for preserving muscle proteins and stimulating new muscle growth without unwanted side effects or limited efficacy has been elusive. Over the last few decades, evidence has emerged through in vitro and in vivo studies that suggest the nuclear receptors REV-ERB and ROR might modulate pathways involved in myogenesis and mitochondrial biogenesis. Hinting that REV-ERB and ROR might be targeted to treat myopathies. However there is still a need for substantial investigation into the roles of these nuclear receptors in in vivo rodent models of degenerative muscle diseases and acute injury. Although exciting, REV-ERB and ROR have somewhat confounding roles in muscle physiology and therefore more studies utilizing in vivo models of skeletal muscle myopathies are needed. In this review we highlight the molecular forces driving some of the major degenerative muscular diseases and showcase two promising molecular targets that may have the potential to treat myopathies: ROR and REV-ERB.

  8. ENDF/B-5 Fission Products Library. Rev. 2

    International Nuclear Information System (INIS)

    Schwerer, O.; Pronyaev, V.G.; Lemmel, H.D.

    1984-07-01

    This document summarizes contents and documentation of the 1984 version of the Fission Products Nuclear Data File of the ENDF/B-5 Library (Rev. 2) maintained by the National Nuclear Data Center (NNDC) at the Brookhaven National Laboratory, USA. This file contains numerical neutron reaction data and decay data for 877 fission product nuclides. The entire file or selective retrievals from it can be obtained on magnetic tape from the IAEA Nuclear Data Section. (author)

  9. Efficacy of the Group Music and Imagery method (GrpMI) for women suffering from fibromyalgia: A randomized controlled trial

    DEFF Research Database (Denmark)

    Pedersen, Inge Nygaard

    2018-01-01

    Abstract Background: Fibromyalgia (FM) affects about 2-4% of the world population. Patients, mostly women, experience chronic widespread pain, fatigue, stiffness, sleep disturbances, and psychological disorders, especially depression and anxiety. Objective: The aim of this study was to assess...... the efficacy of Group Music and Imagery (GrpMI), including relaxation, music listening and spontaneous imagery, for subjective psychological wellbeing, functional capacity and health, pain perception, anxiety and depression in women with FM. Methods: Fifty-six women aged 35 to 65 (M = 51.3) diagnosed with FM...... groups found a significant increase in psychological wellbeing and a reduction in the rest of the variables, whereas the control groups only showed decreases in trait anxiety and trait depression. No significant differences were observed in the control groups at the follow-up, while the experimental...

  10. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export.

    Science.gov (United States)

    Behrens, Ryan T; Aligeti, Mounavya; Pocock, Ginger M; Higgins, Christina A; Sherer, Nathan M

    2017-02-01

    HIV-1's Rev protein forms a homo-oligomeric adaptor complex linking viral RNAs to the cellular CRM1/Ran-GTP nuclear export machinery through the activity of Rev's prototypical leucine-rich nuclear export signal (NES). In this study, we used a functional fluorescently tagged Rev fusion protein as a platform to study the effects of modulating Rev NES identity, number, position, or strength on Rev subcellular trafficking, viral RNA nuclear export, and infectious virion production. We found that Rev activity was remarkably tolerant of diverse NES sequences, including supraphysiological NES (SNES) peptides that otherwise arrest CRM1 transport complexes at nuclear pores. Rev's ability to tolerate a SNES was both position and multimerization dependent, an observation consistent with a model wherein Rev self-association acts to transiently mask the NES peptide(s), thereby biasing Rev's trafficking into the nucleus. Combined imaging and functional assays also indicated that NES masking underpins Rev's well-known tendency to accumulate at the nucleolus, as well as Rev's capacity to activate optimal levels of late viral gene expression. We propose that Rev multimerization and NES masking regulates Rev's trafficking to and retention within the nucleus even prior to RNA binding. HIV-1 infects more than 34 million people worldwide causing >1 million deaths per year. Infectious virion production is activated by the essential viral Rev protein that mediates nuclear export of intron-bearing late-stage viral mRNAs. Rev's shuttling into and out of the nucleus is regulated by the antagonistic activities of both a peptide-encoded N-terminal nuclear localization signal and C-terminal nuclear export signal (NES). How Rev and related viral proteins balance strong import and export activities in order to achieve optimal levels of viral gene expression is incompletely understood. We provide evidence that multimerization provides a mechanism by which Rev transiently masks its NES peptide

  11. Identification of anti-HBV activities in Paeonia suffruticosa Andr. using GRP78 as a drug target on Herbochip®.

    Science.gov (United States)

    Lam, Iao-Fai; Huang, Min; Chang, Margaret Dah-Tysr; Yao, Pei-Wun; Chou, Yu-Ting; Ng, Sim-Kun; Tsai, Ying-Lin; Lin, Yu-Chang; Zhang, Yun-Feng; Yang, Xiao-Yuan; Lai, Yiu-Kay

    2017-01-01

    Herbochip ® technology is a high throughput drug screening platform in a reverse screening manner, in which potential chemical leads in herbal extracts are immobilized and drug target proteins can be used as probes for screening process [BMC Complementary and Alternative Medicine (2015) 15:146]. While herbal medicines represent an ideal reservoir for drug screenings, here a molecular chaperone GRP78 is demonstrated to serve as a potential target for antiviral drug discovery. We cloned and expressed a truncated but fully functional form of human GRP78 (hGRP78 1-508 ) and used it as a probe for anti-HBV drug screening on herbochips. In vitro cytotoxicity and in vitro anti-HBV activity of the herbal extracts were evaluated by MTT and ELISA assays, respectively. Finally, anti-HBV activity was confirmed by in vivo assay using DHBV DNA levels in DHBV-infected ducklings as a model. Primary screenings using GRP78 on 40 herbochips revealed 11 positives. Four of the positives, namely Dioscorea bulbifera , Lasiosphaera fenzlii , Paeonia suffruticosa and Polygonum cuspidatum were subjected to subsequent assays. None of the above extracts was cytotoxic to AML12 cells, but P. cuspidatum extract (PCE) was found to be cytotoxic to HepG2 2.2.15 cells. Both PCE and P. suffruticosa extract (PSE) suppressed secretion of HBsAg and HBeAg in HepG2 2.2.15 cells. The anti-HBV activity of PSE was further confirmed in vivo. We have demonstrated that GRP78 is a valid probe for anti-HBV drug screening on herbochips. We have also shown that PSE, while being non-cytotoxic, possesses in vitro and in vivo anti-HBV activities. Taken together, our data suggest that PSE may be a potential anti-HBV agent for therapeutic use.

  12. Decreased cell survival and DNA repair capacity after UVC irradiation in association with down-regulation of GRP78/BiP in human RSa cells

    International Nuclear Information System (INIS)

    Zhai Ling; Kita, Kazuko; Wano, Chieko; Wu Yuping; Sugaya, Shigeru; Suzuki, Nobuo

    2005-01-01

    In contrast to extensive studies on the roles of molecular chaperones, such as heat shock proteins, there are only a few reports about the roles of GRP78/BiP, an endoplasmic reticulum (ER) stress-induced molecular chaperone, in mammalian cell responses to DNA-damaging stresses. To investigate whether GRP78/BiP is involved in resistance to a DNA-damaging agent, UVC (principally 254 nm in wavelength), we established human cells with down-regulation of GRP78/BiP by transfection of human RSa cells with antisense cDNA for GRP78/BiP. We found that the transfected cells showed higher sensitivity to UVC-induced cell death than control cells transfected with the vector alone. In the antisense-cDNA transfected cells, the removal capacities of the two major types of UVC-damaged DNA (thymine dimers and (6-4) photoproducts) in vivo and DNA synthesis activity of whole cell extracts to repair UVC-irradiated plasmids in vitro were remarkably decreased compared with those in the control cells. Furthermore, the antisense-cDNA transfected cells also showed slightly higher sensitivity to cisplatin-induced cell death than the control cells. Cisplatin-induced DNA damage is primarily repaired by nucleotide excision repair, like UVC-induced DNA damage. The present results suggest that GRP78/BiP plays a protective role against UVC-induced cell death possibly via nucleotide excision repair, at least in the human RSa cells tested

  13. Administrative Circulars No. 12 A (Rev. 2) - "Education fees” and No. 12 B (Rev. 2) - “Education fees and language courses”

    CERN Multimedia

    2013-01-01

    Administrative Circulars No. 12 A (Rev. 2) entitled “Education fees” and No. 12 B (Rev. 2) entitled “Education fees and language courses”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 27 June 2013 and entering into force on 1 August 2013, are available on the intranet site of the Human Resources Department (see here).   Administrative Circular No. 12 A (Rev. 2) is applicable to Staff Members (except former “Local Staff Members”) recruited before 1st January 2007. Administrative Circular No. 12 B (Rev. 2) is applicable to Staff Members recruited on or after 1st January 2007, to Fellows, to Scientific Associates, to Guest Professors and to former “Local Staff” recruited before 1st January 2007. They cancel and replace Administrative Circulars No. 12 A (Rev. 1/Corr.) entitled "Education fees” and No. 12 B (Rev. 1/Corr.) entitled “Edu...

  14. Mutational definition of functional domains within the Rev homolog encoded by human endogenous retrovirus K.

    Science.gov (United States)

    Bogerd, H P; Wiegand, H L; Yang, J; Cullen, B R

    2000-10-01

    Nuclear export of the incompletely spliced mRNAs encoded by several complex retroviruses, including human immunodeficiency virus type 1 (HIV-1), is dependent on a virally encoded adapter protein, termed Rev in HIV-1, that directly binds both to a cis-acting viral RNA target site and to the cellular Crm1 export factor. Human endogenous retrovirus K, a family of ancient endogenous retroviruses that is not related to the exogenous retrovirus HIV-1, was recently shown to also encode a Crm1-dependent nuclear RNA export factor, termed K-Rev. Although HIV-1 Rev and K-Rev display little sequence identity, they share the ability not only to bind to Crm1 and to RNA but also to form homomultimers and shuttle between nucleus and cytoplasm. We have used mutational analysis to identify sequences in the 105-amino-acid K-Rev protein required for each of these distinct biological activities. While mutations in K-Rev that inactivate any one of these properties also blocked K-Rev-dependent nuclear RNA export, several K-Rev mutants were comparable to wild type when assayed for any of these individual activities yet nevertheless defective for RNA export. Although several nonfunctional K-Rev mutants acted as dominant negative inhibitors of K-Rev-, but not HIV-1 Rev-, dependent RNA export, these were not defined by their inability to bind to Crm1, as is seen with HIV-1 Rev. In total, this analysis suggests a functional architecture for K-Rev that is similar to, but distinct from, that described for HIV-1 Rev and raises the possibility that viral RNA export mediated by the approximately 25 million-year-old K-Rev protein may require an additional cellular cofactor that is not required for HIV-1 Rev function.

  15. UV-induced reversion of his4 frameshift mutations in rad6, rev1, and rev3 mutants of yeast.

    Science.gov (United States)

    Lawrence, C W; O'Brien, T; Bond, J

    1984-01-01

    The UV-induced reversion of two his4 frameshift alleles was much reduced in rad6 mutants of Saccharomyces cerevisiae, an observation that is consistent with the hypothesis that RAD6 function is required for the induction of all types of genetic alteration in misrepair mutagenesis. The reversion of these his4 alleles, together with two others of the same type, was also reduced in rev1 and rev3 mutant strains; in these, however, the extent of the reduction varied considerably with test allele used, in a manner analogous to the results in these strains for base repair substitution test alleles. The general features of UV-induced frameshift and substitution mutagenesis therefore appear quite similar, indicating that they may depend on related processes. If this conclusion is correct, greater attention must be given to integrating models which account for the production of nucleotide additions and deletions into those concerning misrepair mutagenesis.

  16. Sampling and estimation techniques for the implementation of new classification systems: the change-over from NACE Rev. 1.1 to NACE Rev. 2 in business surveys

    Directory of Open Access Journals (Sweden)

    Jan van den Brakel

    2010-09-01

    Full Text Available This paper describes some of the methodological problems encountered with the change-over from the NACE Rev. 1.1 to the NACE Rev. 2 in business statistics. Different sampling and estimation strategies are proposed to produce reliable figures for the domains under both classifications simultaneously. Furthermore several methods are described that can be used to reconstruct time series for the domains under the NACE Rev. 2.

  17. Repository Surface Design Engineering Files Report Rev 00 ICN 1

    International Nuclear Information System (INIS)

    2001-01-01

    The objective of the Repository Surface Design Engineering Files Report Supplement [herein known as the Engineering Files (EF)] is to provide the surface design data needed by the Environmental Impact Statement (EIS) contractor to prepare the EIS and evaluate options and alternatives. This document is based on the Repository Surface Design Engineering Files Report, Revision 03 (CRWMS M and O 1999f) (EF Rev 03). Where facility and system designs have been changed for the Site Recommendation (SR) effort they are described in this report. EIS information provided in this report includes the following: (1) Description of program phases; there are no changes that impact this report. (2) A description of the major design requirements and assumptions that drive the surface facilities reference design is provided herein (Section 2.2), including the surface design resulting from recommendations regarding Enhanced Design Alternative (EDA) II, as discussed in the License Application Design Section Report (CRWMS M and O 1999d), and changes to the waste stream. See Section 2, Table 2-2, for the SR waste stream. (3) The major design requirements and assumptions that drive the surface facilities reference design are by reference to EF Rev 03; there are no changes that impact this report. (4) Description of the reference design concept and existing site conditions is by reference to EF Rev 03 (including Table 4-1, which is not included in this supplement); there are no changes that impact this report. (5) Description of alternative design cases is by reference to EF Rev 03; there are no changes that impact this report. (6) Description of optional inventory modules is by reference to EF Rev 03; there are no changes that impact this report. (7) Tabular summary level engineering values (i.e., staffing, wastes, emissions, resources, and land use) for the reference design and the alternative design cases that address construction, emplacement operations, caretaker operations, and

  18. The Craterostigma plantagineum glycine-rich protein CpGRP1 interacts with a cell wall-associated protein kinase 1 (CpWAK1) and accumulates in leaf cell walls during dehydration.

    Science.gov (United States)

    Giarola, Valentino; Krey, Stephanie; von den Driesch, Barbara; Bartels, Dorothea

    2016-04-01

    Craterostigma plantagineum tolerates extreme desiccation. Leaves of this plant shrink and extensively fold during dehydration and expand again during rehydration, preserving their structural integrity. Genes were analysed that may participate in the reversible folding mechanism. Analysis of transcripts abundantly expressed in desiccated leaves identified a gene putatively coding for an apoplastic glycine-rich protein (CpGRP1). We studied the expression, regulation and subcellular localization of CpGRP1 and its ability to interact with a cell wall-associated protein kinase (CpWAK1) to understand the role of CpGRP1 in the cell wall during dehydration. The CpGRP1 protein accumulates in the apoplast of desiccated leaves. Analysis of the promoter revealed that the gene expression is mainly regulated at the transcriptional level, is independent of abscisic acid (ABA) and involves a drought-responsive cis-element (DRE). CpGRP1 interacts with CpWAK1 which is down-regulated in response to dehydration. Our data suggest a role of the CpGRP1-CpWAK1 complex in dehydration-induced morphological changes in the cell wall during dehydration in C. plantagineum. Cell wall pectins and dehydration-induced pectin modifications are predicted to be involved in the activity of the CpGRP1-CpWAK1 complex. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

  19. Alternative Fuels Data Center: New Hampshire Fleet Revs up With Natural Gas

    Science.gov (United States)

    New Hampshire Fleet Revs up With Natural Gas to someone by E-mail Share Alternative Fuels Data Center: New Hampshire Fleet Revs up With Natural Gas on Facebook Tweet about Alternative Fuels Data Center: New Hampshire Fleet Revs up With Natural Gas on Twitter Bookmark Alternative Fuels Data Center

  20. RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs

    International Nuclear Information System (INIS)

    Huang, Feng; Zhang, Junsong; Zhang, Yijun; Geng, Guannan; Liang, Juanran; Li, Yingniang; Chen, Jingliang; Liu, Chao; Zhang, Hui

    2015-01-01

    Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various steps. However, the endogenous MOV10 was required in certain step(s) of HIV-1 replication. In this report, we found that MOV10 potently enhances the nuclear export of viral mRNAs and subsequently increases the expression of Gag protein and other late products through affecting the Rev/RRE axis. The co-immunoprecipitation analysis indicated that MOV10 interacts with Rev in an RNA-independent manner. The DEAG-box of MOV10 was required for the enhancement of Rev/RRE-dependent nuclear export and the DEAG-box mutant showed a dominant-negative activity. Our data propose that HIV-1 utilizes the anti-viral factor MOV10 to function as a co-factor of Rev and demonstrate the complicated effects of MOV10 on HIV-1 life cycle. - Highlights: • MOV10 can function as a co-factor of HIV-1 Rev. • MOV10 facilitates Rev/RRE-dependent transport of viral mRNAs. • MOV10 interacts with Rev in an RNA-independent manner. • The DEAG-box of MOV10 is required for the enhancement of Rev/RRE-dependent export.

  1. RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Feng; Zhang, Junsong; Zhang, Yijun; Geng, Guannan; Liang, Juanran; Li, Yingniang; Chen, Jingliang [Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Liu, Chao, E-mail: liuchao9@mail.sysu.edu.cn [Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Zhang, Hui [Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China)

    2015-12-15

    Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various steps. However, the endogenous MOV10 was required in certain step(s) of HIV-1 replication. In this report, we found that MOV10 potently enhances the nuclear export of viral mRNAs and subsequently increases the expression of Gag protein and other late products through affecting the Rev/RRE axis. The co-immunoprecipitation analysis indicated that MOV10 interacts with Rev in an RNA-independent manner. The DEAG-box of MOV10 was required for the enhancement of Rev/RRE-dependent nuclear export and the DEAG-box mutant showed a dominant-negative activity. Our data propose that HIV-1 utilizes the anti-viral factor MOV10 to function as a co-factor of Rev and demonstrate the complicated effects of MOV10 on HIV-1 life cycle. - Highlights: • MOV10 can function as a co-factor of HIV-1 Rev. • MOV10 facilitates Rev/RRE-dependent transport of viral mRNAs. • MOV10 interacts with Rev in an RNA-independent manner. • The DEAG-box of MOV10 is required for the enhancement of Rev/RRE-dependent export.

  2. Avaliação e seleção de progênies de cupuaçuzeiro (Theobroma grandiflorum, em Belém, Pará Evaluation and selection of Theobroma grandiflorum parents, in Belem, Para

    Directory of Open Access Journals (Sweden)

    Rafael Moysés Alves

    2010-03-01

    Full Text Available Este trabalho teve por objetivo a seleção entre e dentro de progênies de irmãos completos de cupuaçuzeiro. Foram avaliadas 21 progênies de irmãos completos, em dois experimentos, com cinco plantas por parcela. Os experimentos foram avaliados ao nível de indivíduos, em cada safra, para os caracteres: produção de frutos, polpa e semente, bem como resistência à vassoura-de-bruxa. Para efeito de seleção, também foi considerado o grau de parentesco das matrizes. As análises foram conduzidas via metodologia de modelos lineares mistos, como delineamento em blocos incompletos, desbalanceados com tratamentos comuns. Foram estimados os parâmetros genéticos e os valores genotípicos ajustados de progênies, bem como os valores genéticos aditivos e genotípicos individuais. Os resultados demonstraram que as progênies 12; 13; 18; 20; 21 e 24 tiveram os melhores desempenhos para as variáveis de produção. Entre elas, as progênies 12; 13 e 18 foram as que mais se destacaram. Foram selecionadas três matrizes da progênie 18, duas matrizes da progênie 12 e uma matriz de cada uma das progênies 13; 20 e 21. É possível concluir que essas matrizes, juntamente com materiais selecionados em outras áreas experimentais, apresentam potencial para compor um pomar de sementes clonais, estabelecido em lote isolado de outros plantios, onde será produzida uma população melhorada de primeiro ciclo que se constituirá na nova cultivar de cupuaçuzeiro.This paper had as objective the selection among and within full sib families of Theobroma grandiflorum. A total of 21 full sibs progenies were appraised, in two experiments, with five plants for plot. The experiments were appraised at the individuals' level, in each crop, for the traits: production of fruits, pulp and seed and resistance to witch's broom disease. The degree of genetic relationship of the candidates was also considered. The analyses were performed through the methodology of mixed

  3. Antioxidant and ACE-inhibitory activities of hemp (Cannabis sativa L.) protein hydrolysates produced by the proteases AFP, HT, Pro-G, actinidin and zingibain.

    Science.gov (United States)

    Teh, Sue-Siang; Bekhit, Alaa El-Din A; Carne, Alan; Birch, John

    2016-07-15

    Hemp protein isolates (HPIs) were hydrolysed by proteases (AFP, HT, ProG, actinidin and zingibain). The enzymatic hydrolysis of HPIs was evaluated through the degree of hydrolysis and SDS-PAGE profiles. The bioactive properties of the resultant hydrolysates (HPHs) were accessed through ORAC, DPPḢ scavenging and ACE-inhibitory activities. The physical properties of the resultant HPHs were evaluated for their particle sizes, zeta potential and surface hydrophobicity. HT had the highest rate of caseinolytic activity at the lowest concentration (0.1 mg mL(-1)) compared to other proteases that required concentration of 100 mg mL(-1) to achieve their maximum rate of caseinolytic activity. This led to the highest degree of hydrolysis of HPIs by HT in the SDS-PAGE profiles. Among all proteases and substrates, HT resulted in the highest bioactivities (ORAC, DPPḢ scavenging and ACE-inhibitory activities) generated from alkali extracted HPI in the shortest time (2 h) compared to the other protease preparations. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Wind Farm Wake: The Horns Rev Photo Case

    DEFF Research Database (Denmark)

    Hasager, Charlotte Bay; Rasmussen, Leif; Peña, Alfredo

    2013-01-01

    The aim of the paper is to examine the nowadays well-known wind farm wake photographs taken on 12 February 2008 at the offshore Horns Rev 1 wind farm. The meteorological conditions are described from observations from several satellite sensors quantifying clouds, surface wind vectors and sea surf...... in the wake regions with relatively high axial velocities and high turbulent kinetic energy. The wind speed is near cut-in and most turbines produce very little power. The rotational pattern of spiraling bands produces the large-scale structure of the wake fog....

  5. Mapping the binding interface between an HIV-1 inhibiting intrabody and the viral protein Rev.

    Directory of Open Access Journals (Sweden)

    Thomas Vercruysse

    Full Text Available HIV-1 Rev is the key protein in the nucleocytoplasmic export and expression of the late viral mRNAs. An important aspect for its function is its ability to multimerize on these mRNAs. We have recently identified a llama single-domain antibody (Nb190 as the first inhibitor targeting the Rev multimerization function in cells. This nanobody is a potent intracellular antibody that efficiently inhibits HIV-1 viral production. In order to gain insight into the Nb190-Rev interaction interface, we performed mutational and docking studies to map the interface between the nanobody paratope and the Rev epitope. Alanine mutants of the hyper-variable domains of Nb190 and the Rev multimerization domains were evaluated in different assays measuring Nb190-Rev interaction or viral production. Seven residues within Nb190 and five Rev residues are demonstrated to be crucial for epitope recognition. These experimental data were used to perform docking experiments and map the Nb190-Rev structural interface. This Nb190-Rev interaction model can guide further studies of the Nb190 effect on HIV-1 Rev function and could serve as starting point for the rational development of smaller entities binding to the Nb190 epitope, aimed at interfering with protein-protein interactions of the Rev N-terminal domain.

  6. Down-regulation of GRP78 is associated with the sensitivity of chemotherapy to VP-16 in small cell lung cancer NCI-H446 cells

    International Nuclear Information System (INIS)

    Wang, Yingyan; Wang, Wei; Wang, Siyan; Wang, Jiarui; Shao, Shujuan; Wang, Qi

    2008-01-01

    Chemotherapy resistance remains a major obstacle for the treatment of small cell lung cancer (SCLC). Glucose-regulated protein 78 (GRP78), an endoplasmic reticulum chaperone, plays a critical role in chemotherapy resistance in some cancers. However, whether the suppression of the chaperone can enhance the sensitivity of chemotherapy in SCLC is still unclear. The SCLC NCI-H446 cells were divided into three groups: BAPTA-AM→A23187-treated group, A23187-treated group and control-group. Immunofluorescence, western blot and RT-PCR were used to assess the expression of GRP78 at both protein and mRNA levels. Cell apoptosis and the cell cycle distributions of the cells were analyzed by flow cytometry in order to evaluate the therapeutic sensitivity to VP-16. The expression of GRP78 at both protein and mRNA levels in the BAPTA-AM→A23187-treated cells dramatically decreased as compared to that in both A23187-treated and control groups. After treatment by VP-16, the percentage of apoptotic cells in BAPTA-AM→A23187-treated cells were: 33.4 ± 1.01%, 48.2 ± 1.77%, 53.0 ± 1.43%, 56.5 ± 2.13%, respectively, corresponding to the concentrations of BAPTA-AM 10, 15, 25, 40 μM, which was statistically significant high in comparison with the A23187-treated group and untreated-group (7.18 ± 1.03% and 27.8 ± 1.45%, respectively, p < 0.05). The results from analysis of cell cycle distribution showed that there was a significantly decreased in G 1 phase and a dramatically increased in S phase for the BAPTA-AM→A23187-treated cells as compared with the untreated cells. BAPTA-AM is a strong inhibitor of GRP78 in the NCI-H446 cell line, the down-regulation of GRP78 can significantly increase the sensitivity to VP-16. The suppression of GRP78 may offer a new surrogated therapeutic approach to the clinical management of lung cancer

  7. Publication Of Administrative Circulars: No. 4 (Rev. 4) – Unemployment Insurance Scheme No. 30 (Rev. 2) – Financial Benefits on Taking Up Appointment and on Termination of Contract

    CERN Multimedia

    HR Department

    2008-01-01

    Administrative Circular No. 4 (Rev. 4) – Unemployment insurance scheme Administrative Circular No. 4 (Rev. 4) – "Unemployment insurance scheme", approved following discussion in the Standing Concertation Committee meetings of 28 August 2007 and 27 February 2008, is now available on the intranet site of the Human Resources Department. It cancels and replaces Administrative Circular No. 4 (Rev. 3) – "Unemployment insurance" of October 1993. Copies will shortly be available in Departmental secretariats. Human Resources Department Tel. 78003 Administrative Circular No. 30 (Rev. 2) – Financial benefits on taking up appointment and termination of contract Administrative Circular No. 30 (Rev. 2) – "Financial benefits on taking up appointment and termination of contract", approved following discussion in the Standing Concertation Committee meetings of 28 August 2007 and 27 February 2008, is now available on the intranet site of the Human Resources De...

  8. Publication Of Administrative Circulars: No. 4 (Rev. 4) – Unemployment Insurance Scheme No. 30 (Rev. 2) – Financial Benefits on Taking Up Appointment and on Termination of Contract

    CERN Document Server

    HR Department

    2008-01-01

    Administrative Circular No. 4 (Rev. 4) – Unemployment insurance scheme Administrative Circular No. 4 (Rev. 4) – "Unemployment insurance scheme", approved following discussion at the Standing Concertation Committee meetings of 28 August 2007 and 27 February 2008, is now available on the intranet site of the Human Resources Department. It cancels and replaces Administrative Circular No. 4 (Rev. 3) – "Unemployment insurance" of October 1993. Copies will shortly be available in Departmental secretariats. Human Resources Department Tel. 78003 Administrative Circular No. 30 (Rev. 2) – Financial benefits on taking up appointment and termination of contract Administrative Circular No. 30 (Rev. 2) – "Financial benefits on taking up appointment and termination of contract", approved following discussion at the Standing Concertation Committee meetings of 28 August 2007 and 27 February 2008, is now available on the intranet site of the Human Resources De...

  9. 12MW Horns Rev experiment[Wind farm

    Energy Technology Data Exchange (ETDEWEB)

    Hasager, C.B.; Pena, A; Mikkelsen, T.; Courtney, M.; Antoniou, I.; Gryning, S.-E.; Hansen, P. [Risoe National Lab., DTU, Wind Energy Dept. (Denmark); Soerensen, P.B. [DONG Energy (Denmark)

    2007-10-15

    The 12MW project with the full title '12 MW wind turbines: the scientific basis for their operation at 70 to 270 m height offshore' has the goal to experimentally investigate the wind and turbulence characteristics between 70 and 270 m above sea level and thereby establish the scientific basis relevant for the next generation of huge 12 MW wind turbines operating offshore. The report describes the experimental campaign at the Horns Rev offshore wind farm at which observations from Doppler Laser LIDAR and SODAR were collected from 3 May to 24 October 2006. The challenges for mounting and operating the instruments on the transformer platform at Horns Rev were overcome by a close collaboration between DONG energy and Risoe National Laboratory DTU. The site is presented. In particular, three tall offshore meteorological masts, up to 70 m tall, provided a useful source of meteorological data for comparison to the remotely sensed wind and turbulence observations. The comparison showed high correlation. The LIDAR and SODAR wind and turbulence observations were collected far beyond the height of the masts (up to 160 m above sea level) and the extended profiles were compared to the logarithmic wind profile. Further studies on this part of the work are on-going. Technical detail on LIDAR and SODAR are provided as well as theoretical work on turbulence and atmospheric boundary layer flow. Selected results from the experimental campaign are reported. (au)

  10. Wind Farm Wake: The 2016 Horns Rev Photo Case

    Directory of Open Access Journals (Sweden)

    Charlotte Bay Hasager

    2017-03-01

    Full Text Available Offshore wind farm wakes were observed and photographed in foggy conditions at Horns Rev 2 on 25 January 2016 at 12:45 UTC. These new images show highly contrasting conditions regarding the wind speed, turbulence intensity, atmospheric stability, weather conditions and wind farm wake development as compared to the Horns Rev 1 photographs from 12 February 2008. The paper examines the atmospheric conditions from satellite images, radiosondes, lidar and wind turbine data and compares the observations to results from atmospheric meso-scale modelling and large eddy simulation. Key findings are that a humid and warm air mass was advected from the southwest over cold sea and the dew-point temperature was such that cold-water advection fog formed in a shallow layer. The flow was stably stratified and the freestream wind speed was 13 m/s at hub height, which means that most turbines produced at or near rated power. The wind direction was southwesterly and long, narrow wakes persisted several rotor diameters downwind of the wind turbines. Eventually mixing of warm air from aloft dispersed the fog in the far wake region of the wind farm.

  11. rev piyasaları ve vergilendirilmesi

    OpenAIRE

    İşler, İsmail; Utku, Mehmet

    2015-01-01

    rev piyasaları kavramı, vadeli işlem sözleşmeleri (forward, futures), opsiyon sözleşmeleri, swap sözleşmeleri ve aracı kuruluş varantları işlemlerinin tamamını içermektedir. Bu tür işlemler vadeli işlemlerdir. Vadeli işlemlerin ortak özelliği, ilerideki bir tarihte teslimatı yapılmak üzere herhangi bir malın veya finansal aracın, bugünden alım satımının yapılmasıdır. Bu çalışmada türev araçların tanımları ve vergilendirilmeleri örneklerle açıklanacaktır. The term derivatives market invol...

  12. Initiation of lambda DNA replication. The Escherichia coli small heat shock proteins, DnaJ and GrpE, increase DnaK's affinity for the lambda P protein.

    Science.gov (United States)

    Osipiuk, J; Georgopoulos, C; Zylicz, M

    1993-03-05

    It is known that the initiation of bacteriophage lambda replication requires the orderly assembly of the lambda O.lambda P.DnaB helicase protein preprimosomal complex at the ori lambda DNA site. The DnaK, DnaJ, and GrpE heat shock proteins act together to destabilize the lambda P.DnaB complex, thus freeing DnaB and allowing it to unwind lambda DNA near the ori lambda site. The first step of this disassembly reaction is the binding of DnaK to the lambda P protein. In this report, we examined the influence of the DnaJ and GrpE proteins on the stability of the lambda P.DnaK complex. We present evidence for the existence of the following protein-protein complexes: lambda P.DnaK, lambda P.DnaJ, DnaJ.DnaK, DnaK.GrpE, and lambda P.DnaK.GrpE. Our results suggest that the presence of GrpE alone destabilizes the lambda P.DnaK complex, whereas the presence of DnaJ alone stabilizes the lambda P.DnaK complex. Using immunoprecipitation, we show that in the presence of GrpE, DnaK exhibits a higher affinity for the lambda P.DnaJ complex than it does alone. Using cross-linking with glutaraldehyde, we show that oligomeric forms of DnaK exhibit a higher affinity for lambda P than monomeric DnaK. However, in the presence of GrpE, monomeric DnaK can efficiently bind lambda P protein. These findings help explain our previous results, namely that in the GrpE-dependent lambda DNA replication system, the DnaK protein requirement can be reduced up to 10-fold.

  13. Macular Sensitivity Measured With Microperimetry in Stargardt Disease in the Progression of Atrophy Secondary to Stargardt Disease (ProgStar) Study: Report No. 7.

    Science.gov (United States)

    Schönbach, Etienne M; Wolfson, Yulia; Strauss, Rupert W; Ibrahim, Mohamed A; Kong, Xiangrong; Muñoz, Beatriz; Birch, David G; Cideciyan, Artur V; Hahn, Gesa-Astrid; Nittala, Muneeswar; Sunness, Janet S; Sadda, SriniVas R; West, Sheila K; Scholl, Hendrik P N

    2017-07-01

    New outcome measures for treatment trials for Stargardt disease type 1 (STGD1) and other macular diseases are needed. Microperimetry allows mapping of light sensitivity of the macula and provides topographic information on visual function beyond visual acuity. To measure and analyze retinal light sensitivity of the macula in STGD1 using fundus-controlled perimetry (microperimetry). This was a multicenter prospective cohort study. A total of 199 patients and 326 eyes with molecularly confirmed (ABCA4) STGD1 underwent testing with the Nidek MP-1 microperimeter as part of the multicenter, prospective Natural History of the Progression of Atrophy Secondary to Stargardt Disease (ProgStar) study. Sensitivity of 68 retinal loci was tested, and the mean sensitivity (MS) was determined; each point was categorized as "normal," "relative," or "deep" scotoma. Mean sensitivity and the number of points with normal sensitivity, relative, or deep scotomas. Mean (SD) patient age was 34.2 (14.7) years, mean (SD) best-corrected visual acuity of all eyes was 47.8 (16.9) Early Treatment Diabetic Retinopathy Study letter score (approximately 20/100 Snellen equivalent), and mean MS of all eyes of all 68 points was 11.0 (5.0) dB. The median number of normal points per eye was 49 (mean [SD], 41.3 [20.8]; range, 0-68); abnormal sensitivity and deep scotomas were more prevalent in the central macula. Mean sensitivity was lower in the fovea (mean [SD], 2.7 [4.4] dB) than in the inner (mean [SD], 6.8 [5.8] dB) and outer ring (mean [SD], 12.7 [5.3] dB). Overall MS per eye was 0.086 dB lower per year of additional age (95% CI, -0.13 to -0.041; P clinical trials.

  14. Applications of the INFCIRC225/rev5 to the national physical protection regime

    International Nuclear Information System (INIS)

    Kim, Jae Gwang

    2011-01-01

    The IAEA's first effort for playing a key role in physical protection of nuclear material and facilities resulted in the publication of 'Recommendations for the physical protection of nuclear material' in 1972. These recommendations were revised by a group of experts in co-operation with the IAEA Secretariat and the revised version was published in 1975 in INFCIRC/225. The document was subsequently revised in 1977(rev.1), 1989(rev.2), in 1993(rev.3) and 1998(rev4). The Physical Protection regime in ROK has been established on the basis of INFCIRC225 rev4 since 2004 IAEA recently published The INFCIRC 225 rev5 through the 2 years expert consultations. The publication recommended 12 nuclear security fundamentals and requirements of physical protection of nuclear material and nuclear facility to the member states. This paper suggests the several applications to the national physical protection system from the 12 fundamentals and the requirements

  15. Publication of administrative circular No. 26 (Rev. 8) – Recognition of merit

    CERN Multimedia

    HR Department

    2008-01-01

    Administrative Circular No. 26 (Rev. 8) – Recognition of merit Administrative Circular No. 26 (Rev. 8) – "Recognition of merit", approved by the Standing Concertation Committee at its meeting on 3 September 2008, is now available on the intranet site of the Human Resources Department. It cancels and replaces Administrative Circular No. 26 (Rev. 7) – "Recognition of merit of staff members" of May 2007. Paper copies will shortly be available in Departmental secretariats. Human Resources Department Tel. 78003

  16. Publication of administrative circular No. 26 (Rev. 8) – Recognition of merit

    CERN Multimedia

    HR Department

    2008-01-01

    Administrative Circular No. 26 (Rev. 8) – Recognition of merit Administrative Circular No. 26 (Rev. 8) – "Recognition of merit", approved by the Standing Concertation Committee meeting on 3 September 2008, is now available on the intranet site of the Human Resources Department. It cancels and replaces Administrative Circular No. 26 (Rev. 7) – "Recognition of merit of staff members" of May 2007. Paper copies will shortly be available in Departmental secretariats. Human Resources Department Tel. 78003

  17. Biokinetics and dosimetry of {sup 99m} Tc-EDDA/HYNIC-[Lys{sup 3}]-bombesin in humans: imaging of GRP receptors

    Energy Technology Data Exchange (ETDEWEB)

    Santos C, C.L.; Ferro F, G. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico); Murphy, C.A de [INCMNSZ, 14000 Mexico D.F. (Mexico); Cardena, E.; Pichardo R, P. [Departamento de Medicina Nuclear, Oncologia Centro Medico Siglo XXI, Mexico D.F. (Mexico)

    2007-07-01

    Full text: Bombesin (BN) receptor subtype 2 (GRP-r) is over-expressed on various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently we reported the {sup 99-}mTc-EDDA/HYNIC-[Lys{sup 3}]-Bombesin ({sup 99m}Tc-HYNIC-BN) complex as a new radiopharmaceutical with high stability in human serum, specific cell GRP-receptor binding and rapid internalization. The aim of this study was to evaluate the {sup 99m}Tc-HYNIC-BN biokinetics and dosimetry in 5-healthy and 3-breast cancer women. Whole-body images were acquired at 20, 90, 180 min and 24 h after {sup 99m}Tc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source' organs on each time frame. The same set of ROIs was used for all 8 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-HYNIC-BN time activity curves in each organ, to calculate the total number of disintegrations (N) that occurred in the source regions. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Images showed a rapid radiopharmaceutical blood clearance with predominantly renal excretion and minimal hepatobiliary elimination. {sup 99m}Tc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in breast cancer lesions and well differentiated from GRP-r expression in lungs and airways with normal GRP-r density (ratio 3:1). The equivalent doses for a study using 370 MBq were 7.38{+-}1.68, 0.59{+-}0.08, 2.07{+-}0.60, 0.58{+-}0.1, 0.75{+-}0.09 and 0.43{+-}0.07 mSv for kidneys, liver, lungs, ovaries, pancreas and red marrow respectively. The effective dose was 1.64{+-}0.25 mSv which is comparable with the doses known for most of the {sup 99m}Tc radiopharmaceutical studies in nuclear medicine. (Author)

  18. REV7, a new gene concerned with UV mutagenesis in yeast

    International Nuclear Information System (INIS)

    Lawrence, C.W.; Das, G.; Christensen, R.B.

    1985-01-01

    Three allelic mutations of a new yeast gene, which we have named REV7, have been isolated by testing 313 methyl methane sulfonate sensitive mutants for UV-induced reversion of a lys2 allele. Rev7 mutants are markedly deficient with respect to UV-induced reversion of lys2, are slightly sensitive to UV and appear to be in the RAD6 epistasis group for UV survival. Rev7-1, which is probably an amber mutation, does not appear to affect sporulation in homozygous diploids. The REV7 gene is located about 12 cM distal to HIS5 on chromosome IX. (orig.)

  19. REV7, a new gene concerned with UV mutagenesis in yeast

    Energy Technology Data Exchange (ETDEWEB)

    Lawrence, C.W.; Das, G.; Christensen, R.B.

    1985-06-26

    Three allelic mutations of a new yeast gene, which we have named REV7, have been isolated by testing 313 methyl methane sulfonate sensitive mutants for UV-induced reversion of a lys2 allele. REV7 mutants are markedly deficient with respect to UV-induced reversion of lys2, are slightly sensitive to UV and appear to be in the RAD6 epistasis group for UV survival. Rev7-1, which is probably an amber mutation, does not appear to affect sporulation in homozygous diploids. The REV7 gene is located about 12 cM distal to HIS5 on chromosome IX.

  20. Epistatic participation of REV1 and REV3 in the formation of UV-induced frameshift mutations in cell cycle-arrested yeast cells

    Energy Technology Data Exchange (ETDEWEB)

    Heidenreich, Erich [Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria)]. E-mail: erich.heidenreich@meduniwien.ac.at; Eisler, Herfried [Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria); Steinboeck, Ferdinand [Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria)

    2006-01-29

    Mutations arising in times of cell cycle arrest may provide a selective advantage for unicellular organisms adapting to environmental changes. For multicellular organisms, however, they may pose a serious threat, in that such mutations in somatic cells contribute to carcinogenesis and ageing. The budding yeast Saccharomyces cerevisiae presents a convenient model system for studying the incidence and the mechanisms of stationary-phase mutation in a eukaryotic organism. Having studied the emergence of frameshift mutants after several days of starvation-induced cell cycle arrest, we previously reported that all (potentially error-prone) translesion synthesis (TLS) enzymes identified in S. cerevisiae did not contribute to the basal level of spontaneous stationary-phase mutations. However, we observed that an increased frequency of stationary-phase frameshift mutations, brought about by a defective nucleotide excision repair (NER) pathway or by UV irradiation, was dependent on Rev3p, the catalytic subunit of the TLS polymerase zeta (Pol {zeta}). Employing the same two conditions, we now examined the effect of deletions of the genes coding for polymerase eta (Pol {eta}) (RAD30) and Rev1p (REV1). In a NER-deficient strain background, the increased incidence of stationary-phase mutations was only moderately influenced by a lack of Pol {eta} but completely reduced to wild type level by a knockout of the REV1 gene. UV-induced stationary-phase mutations were abundant in wild type and rad30{delta} strains, but substantially reduced in a rev1{delta} as well as a rev3{delta} strain. The similarity of the rev1{delta} and the rev3{delta} phenotype and an epistatic relationship evident from experiments with a double-deficient strain suggests a participation of Rev1p and Rev3p in the same mutagenic pathway. Based on these results, we propose that the response of cell cycle-arrested cells to an excess of exo- or endogenously induced DNA damage includes a novel replication

  1. Seleção de progênies de meio-irmãos de milho para eficiência no uso de nitrogênio Selection of half-sib of maize for nitrogen use efficiency

    Directory of Open Access Journals (Sweden)

    Rafael Heinz

    2012-12-01

    Full Text Available O trabalho teve por objetivo estimar os parâmetros e os ganhos genéticos na seleção de progênies de meio-irmãos para eficiência no uso de nitrogênio, por meio de seleção simultânea de caracteres. Foram instalados dois experimentos, diferenciados pela adubação nitrogenada, sendo um com adubação adequada (alto N e outro em condição de estresse (baixo N. O delineamento experimental utilizado foi em látice 10x10, com duas repetições. Nos ensaios foram utilizadas 84 progênies de meio-irmãos e 16 testemunhas. A partir dos quadrados médios das análises de variâncias de cada ambiente foram realizadas as estimativas dos parâmetros genéticos. Após as análises, foram selecionadas em cada ambiente 17 progênies de meio-irmãos, por meio do índice clássico de seleção, e verificou-se a repetibilidade das progênies entre os ambientes. Observou-se variabilidade entre as progênies, determinando o potencial da população para a seleção considerando-se a eficiência no uso de nitrogênio. As estimativas de parâmetros e ganhos genéticos foram maiores em alto N. O uso do índice de seleção promoveu estimativas de ganho por seleção de 24,43 e 21,05% para alto e baixo N, respectivamente, para a produtividade de grãos. As progênies selecionadas apresentaram médias de produtividade de grãos de 13,45 e 19,47% maiores que as testemunhas, em alto e baixo N, respectivamente.The study aimed to estimate the parameters and the genetic gain in selection of half-sib for nitrogen use efficiency, through simultaneous selection of characters. Two experiments were established, differentiated by nitrogen fertilization, one with proper fertilization (high N and another in stress conditions (low N. The experiment was using a 10x10 lattice with two replications. In the tests we used 84 different half-sib and 16 witnesses. From the mean squares of analysis of variance in each environment were the estimates of genetic parameters. After the

  2. DynaProg for Scala

    OpenAIRE

    Coppey, Thierry

    2013-01-01

    Dynamic programming is an algorithmic technique to solve problems that follow the Bellman’s principle: optimal solutions depends on optimal sub-problem solutions. The core idea behind dynamic programming is to memoize intermediate results into matrices to avoid multiple computations. Solving a dynamic programming problem consists of two phases: filling one or more matrices with intermediate solutions for sub-problems and recomposing how the final result was constructed (backtracking). In text...

  3. Ploidia e fertilidade de pólen em progênies de citros Ploidy and pollen fertility in citrus hybrids

    Directory of Open Access Journals (Sweden)

    Eduardo Cesar Brugnara

    2008-01-01

    Full Text Available Este trabalho foi desenvolvido para estimar a fertilidade do pólen e determinar o nível de ploidia em progênies dos cruzamentos da tangerineira 'Montenegrina' (C. deliciosa Ten. com a tangerineira 'King' (C. nobilis Lour. e com a laranjeira 'Caipira' (C. sinensis (L. Osb.. As plantas, de pés-francos e com idade entre 11 e 12 anos, são mantidas na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, em Eldorado do Sul. Avaliaram-se o nível de ploidia, através da contagem dos cromossomos em células em meiose, e a fertilidade do pólen, por coloração do pólen com carmim propiônico, em 2005 e 2006. Todos os híbridos avaliados são diplóides e a fertilidade de pólen variou de zero a 98%. C27 - híbrido de 'Montenegrina' x 'Caipira' - revelou-se praticamente estéril, e a fertilidade de C21, do mesmo cruzamento, é de 10%. A menor fertilidade observada no cruzamento da 'Montenegrina' x 'King' foi de 42%.This work was performed to estimate pollen fertility and determinate the ploidy level of 11 to 12 years old progenies of crosses of 'Montenegrina' mandarin (Citrus deliciosa Ten. with 'King' mandarin (C. nobilis Lour. and with 'Caipira' sweet orange (C. sinensis (L. Osb. maintained at Estação Experimental Agronômica of UFRGS, in Eldorado do Sul, Brazil. The ploidy level was determined by chromosome countings in meiosis cells and pollen fertility evaluated by staining pollen grains with propionic carmine. All evaluated hybrids are diploid and pollen fertility varied from zero to 98%. C27, a hybrid 'Montenegrina' x 'Caipira', was found is sterile, and C21, from the same cross, showed 10 % fertility. The lowest observed fertility in the progeny 'Montenegrina' x 'King' was 42 %.

  4. SSC-K code users manual (rev.1)

    International Nuclear Information System (INIS)

    Kwon, Y. M.; Lee, Y. B.; Chang, W. P.; Hahn, D.

    2002-01-01

    The Supper System Code of KAERI (SSC-K) is a best-estimate system code for analyzing a variety of off-normal or accidents in the heat transport system of a pool type LMR design. It is being developed at Korea Atomic Energy Research Institution (KAERI) on the basis of SSC-L, originally developed at BNL to analyze loop-type LMR transients. SSC-K can handle both designs of loop and pool type LMRs. SSC-K contains detailed mechanistic models of transient thermal, hydraulic, neutronic, and mechanical phenomena to describe the response of the reactor core, coolant, fuel elements, and structures to accident conditions. This report provides a revised User's Manual (rev.1) of the SSC-K computer code, focusing on phenomenological model descriptions for new thermal, hydraulic, neutronic, and mechanical modules. A comprehensive description of the models for pool-type reactor is given in Chapters 2 and 3; the steady-state plant characterization, prior to the initiation of transient is described in Chapter 2 and their transient counterparts are discussed in Chapter 3. Discussions on the intermediate heat exchanger (IHX) and the electromagnetic (EM) pump are described in Chapter 4 and 5, respectively. A model of passive safety decay heat removal system (PSDRS) is discussed in Chapter 6, and models for various reactivity feedback effects are discussed in Chapter 7. In Chapter 8, constitutive laws and correlations required to execute the SSC-K are described. New models developed for SSC-K rev.1 are two dimensional hot pool model in Chapter 9, and long term cooling model in Chapter 10. Finally, a brief description of MINET code adopted to simulate BOP is presented in Chapter 11. Based on test runs for typical LMFBR accident analyses, it was found that the present version of SSC-K would be used for the safety analysis of KALIMER. However, the further validation of SSC-K is required for real applications. It is noted that the user's manual of SSC-K will be revised later with the

  5. Joining of hybrid AA6063-6SiCp-3Grp composite and AISI 1030 steel by friction welding

    Directory of Open Access Journals (Sweden)

    N. Rajesh Jesudoss Hynes

    2017-10-01

    Full Text Available Joining of metals and aluminium hybrid metal matrix composites has significant applications in aviation, ship building and automotive industries. In the present work, investigation is carried out on Friction Welding of AISI 1030 steel and hybrid AA6063-6SiCp-3Grpcomposite, that are difficult to weld by fusion welding technique. Silicon carbide and graphite particle reinforced AA6063 matrix hybrid composite was developed successfully using stir casting method and the joining feasibility of AISI1030 steel with AA6063-6SiCp-3Grp hybrid composite was tried out by friction stud welding technique. During friction stage of welding process, the particulates (SiC & Graphite used for reinforcement, tend to increase the viscosity and lead to improper mixing of matrix and reinforcement. This eventually results in lower strength in dissimilar joints. To overcome this difficulty AA1100 interlayer is used while joining hybrid composite to AISI 1030 steel. Experimentation was carried out using Taguchi based design of experiments (DOE technique. Multiple regression methods were applied to understand the relationship between process parameters of the friction stud welding process. Micro structural examination reveals three separate zones namely fully plasticized zone, partially deformed zone and unaffected base material zone. Ultra fine dynamically recrystallized grains of about 341 nm were observed at the fully plasticized zone. EDX analysis confirms the presence of intermetallic compound Fe2Al5 at the joint interface. According to the experimental analysis using DOE, rotational speed and interlayer sheet thickness contribute about 39% and 36% respectively in determining the impact strength of the welded joints. It is found that joining with 0.5 mm interlayer sheet provides efficient joints. Developed regression model could be used to predict the axial shortening distance and impact strength of the welded joint with reasonable accuracy.

  6. Molecular interaction between K-Ras and H-REV107 in the Ras signaling pathway.

    Science.gov (United States)

    Han, Chang Woo; Jeong, Mi Suk; Jang, Se Bok

    2017-09-16

    Ras proteins are small GTPases that serve as master moderators of a large number of signaling pathways involved in various cellular processes. Activating mutations in Ras are found in about one-third of cancers. H-REV107, a K-Ras binding protein, plays an important role in determining K-Ras function. H-REV107 is a member of the HREV107 family of class II tumor suppressor genes and a growth inhibitory Ras target gene that suppresses cellular growth, differentiation, and apoptosis. Expression of H-REV107 was strongly reduced in about 50% of human carcinoma cell lines. However, the specific molecular mechanism by which H-REV107 inhibits Ras is still unknown. In the present study, we suggest that H-REV107 forms a strong complex with activating oncogenic mutation Q61H K-Ras from various biochemical binding assays and modeled structures. In addition, the interaction sites between K-Ras and H-REV107 were predicted based on homology modeling. Here, we found that some structure-based mutants of the K-Ras disrupted the complex formation with H-REV107. Finally, a novel molecular mechanism describing K-Ras and H-REV107 binding is suggested and insights into new K-Ras effector target drugs are provided. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. RevManHAL: towards automatic text generation in systematic reviews.

    Science.gov (United States)

    Torres Torres, Mercedes; Adams, Clive E

    2017-02-09

    Systematic reviews are a key part of healthcare evaluation. They involve important painstaking but repetitive work. A major producer of systematic reviews, the Cochrane Collaboration, employs Review Manager (RevMan) programme-a software which assists reviewers and produces XML-structured files. This paper describes an add-on programme (RevManHAL) which helps auto-generate the abstract, results and discussion sections of RevMan-generated reviews in multiple languages. The paper also describes future developments for RevManHAL. RevManHAL was created in Java using NetBeans by a programmer working full time for 2 months. The resulting open-source programme uses editable phrase banks to envelop text/numbers from within the prepared RevMan file in formatted readable text of a chosen language. In this way, considerable parts of the review's 'abstract', 'results' and 'discussion' sections are created and a phrase added to 'acknowledgements'. RevManHAL's output needs to be checked by reviewers, but already, from our experience within the Cochrane Schizophrenia Group (200 maintained reviews, 900 reviewers), RevManHAL has saved much time which is better employed thinking about the meaning of the data rather than restating them. Many more functions will become possible as review writing becomes increasingly automated.

  8. Wind Farm Wake: The Horns Rev Photo Case

    Directory of Open Access Journals (Sweden)

    Pierre-Elouan Réthoré

    2013-02-01

    Full Text Available The aim of the paper is to examine the nowadays well-known wind farm wake photographs taken on 12 February 2008 at the offshore Horns Rev 1 wind farm. The meteorological conditions are described from observations from several satellite sensors quantifying clouds, surface wind vectors and sea surface temperature as well as ground-based information at and near the wind farm, including Supervisory Control and Data Acquisition (SCADA data. The SCADA data reveal that the case of fog formation occurred 12 February 2008 on the 10:10 UTC. The fog formation is due to very special atmospheric conditions where a layer of cold humid air above a warmer sea surface re-condensates to fog in the wake of the turbines. The process is fed by warm humid air up-drafted from below in the counter-rotating swirl generated by the clock-wise rotating rotors. The condensation appears to take place primarily in the wake regions with relatively high axial velocities and high turbulent kinetic energy. The wind speed is near cut-in and most turbines produce very little power. The rotational pattern of spiraling bands produces the large-scale structure of the wake fog.

  9. HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3

    International Nuclear Information System (INIS)

    Kula, Anna; Gharu, Lavina; Marcello, Alessandro

    2013-01-01

    Human immunodeficiency virus gene expression and replication are regulated at several levels. Incompletely spliced viral RNAs and full-length genomic RNA contain the RRE element and are bound by the viral trans-acting protein Rev to be transported out of the nucleus. Previously we found that the nuclear matrix protein MATR3 was a cofactor of Rev-mediated RNA export. Here we show that the pleiotropic protein PSF binds viral RNA and is associated with MATR3. PSF is involved in the maintenance of a pool of RNA available for Rev activity. However, while Rev and PSF bind the viral pre-mRNA at the site of viral transcription, MATR3 interacts at a subsequent step. We propose that PSF and MATR3 define a novel pathway for RRE-containing HIV-1 RNAs that is hijacked by the viral Rev protein.

  10. Caffeine Abolishes the Ultraviolet-Induced REV3 Translesion Replication Pathway in Mouse Cells

    Directory of Open Access Journals (Sweden)

    Kouichi Yamada

    2011-11-01

    Full Text Available When a replicative DNA polymerase stalls upon encountering a photoproduct on the template strand, it is relieved by other low-processivity polymerase(s, which insert nucleotide(s opposite the lesion. Using an alkaline sucrose density gradient sedimentation technique, we previously classified this process termed UV-induced translesion replication (UV-TLS into two types. In human cancer cells or xeroderma pigmentosum variant (XP-V cells, UV-TLS was inhibited by caffeine or proteasome inhibitors. However, in normal human cells, the process was insensitive to these reagents. Reportedly, in yeast or mammalian cells, REV3 protein (a catalytic subunit of DNA polymerase ζ is predominantly involved in the former type of TLS. Here, we studied UV-TLS in fibroblasts derived from the Rev3-knockout mouse embryo (Rev3KO-MEF. In the wild-type MEF, UV-TLS was slow (similar to that of human cancer cells or XP-V cells, and was abolished by caffeine or MG-262. In 2 cell lines of Rev3KO-MEF (Rev3−/− p53−/−, UV-TLS was not observed. In p53KO-MEF, which is a strict control for Rev3KO-MEF, the UV-TLS response was similar to that of the wild-type. Introduction of the Rev3 expression plasmid into Rev3KO-MEF restored the UV-TLS response in selected stable transformants. In some transformants, viability to UV was the same as that in the wild-type, and the death rate was increased by caffeine. Our findings indicate that REV3 is predominantly involved in UV-TLS in mouse cells, and that the REV3 translesion pathway is suppressed by caffeine or proteasome inhibitors.

  11. Mining the Human Complexome Database Identifies RBM14 as an XPO1-Associated Protein Involved in HIV-1 Rev Function

    OpenAIRE

    Budhiraja, Sona; Liu, Hongbing; Couturier, Jacob; Malovannaya, Anna; Qin, Jun; Lewis, Dorothy E.; Rice, Andrew P.

    2015-01-01

    By recruiting the host protein XPO1 (CRM1), the HIV-1 Rev protein mediates the nuclear export of incompletely spliced viral transcripts. We mined data from the recently described human nuclear complexome to identify a host protein, RBM14, which associates with XPO1 and Rev and is involved in Rev function. Using a Rev-dependent p24 reporter plasmid, we found that RBM14 depletion decreased Rev activity and Rev-mediated enhancement of the cytoplasmic levels of unspliced viral transcripts. RBM14 ...

  12. Membrane docking geometry of GRP1 PH domain bound to a target lipid bilayer: an EPR site-directed spin-labeling and relaxation study.

    Directory of Open Access Journals (Sweden)

    Huai-Chun Chen

    Full Text Available The second messenger lipid PIP(3 (phosphatidylinositol-3,4,5-trisphosphate is generated by the lipid kinase PI3K (phosphoinositide-3-kinase in the inner leaflet of the plasma membrane, where it regulates a broad array of cell processes by recruiting multiple signaling proteins containing PIP(3-specific pleckstrin homology (PH domains to the membrane surface. Despite the broad importance of PIP(3-specific PH domains, the membrane docking geometry of a PH domain bound to its target PIP(3 lipid on a bilayer surface has not yet been experimentally determined. The present study employs EPR site-directed spin labeling and relaxation methods to elucidate the membrane docking geometry of GRP1 PH domain bound to bilayer-embedded PIP(3. The model target bilayer contains the neutral background lipid PC and both essential targeting lipids: (i PIP(3 target lipid that provides specificity and affinity, and (ii PS facilitator lipid that enhances the PIP(3 on-rate via an electrostatic search mechanism. The EPR approach measures membrane depth parameters for 18 function-retaining spin labels coupled to the PH domain, and for calibration spin labels coupled to phospholipids. The resulting depth parameters, together with the known high resolution structure of the co-complex between GRP1 PH domain and the PIP(3 headgroup, provide sufficient constraints to define an optimized, self-consistent membrane docking geometry. In this optimized geometry the PH domain engulfs the PIP(3 headgroup with minimal bilayer penetration, yielding the shallowest membrane position yet described for a lipid binding domain. This binding interaction displaces the PIP(3 headgroup from its lowest energy position and orientation in the bilayer, but the headgroup remains within its energetically accessible depth and angular ranges. Finally, the optimized docking geometry explains previous biophysical findings including mutations observed to disrupt membrane binding, and the rapid lateral

  13. GRP1 PH Domain, Like AKT1 PH Domain, Possesses a Sentry Glutamate Residue Essential for Specific Targeting to Plasma Membrane PI(3,4,5)P3

    Science.gov (United States)

    Pilling, Carissa; Landgraf, Kyle E.; Falke, Joseph J.

    2011-01-01

    During the appearance of the signaling lipid PI(3,4,5)P3, an important subset of pleckstrin homology (PH) domains target signaling proteins to the plasma membrane. To ensure proper pathway regulation, such PI(3,4,5)P3-specific PH domains must exclude the more prevalant, constitutive plasma membrane lipid PI(4,5)P2 and bind the rare PI(3,4,5)P3 target lipid with sufficiently high affinity. Our previous study of the E17K mutant of protein kinase B (AKT1) PH domain, together with evidence from Carpten et al (1), revealed that the native AKT1 E17 residue serves as a sentry glutamate that excludes PI(4,5)P2, thereby playing an essential role in specific PI(3,4,5)P3 targeting (2). The sentry glutamate hypothesis proposes that an analogous sentry glutamate residue is a widespread feature of PI(3,4,5)P3-specific PH domains, and that charge reversal mutation at the sentry glutamate position will yield both increased PI(4,5)P2 affinity and constitutive plasma membrane targeting. To test this hypothesis the present study investigates the E345 residue, a putative sentry glutamate, of General Receptor for Phosphoinositides 1 (GRP1) PH domain. The results show that incorporation of the E345K charge reversal mutation into GRP1 PH domain enhances PI(4,5)P2 affinity 8-fold and yields constitutive plasma membrane targeting in cells, reminiscent of the effects of the E17K mutation in AKT1 PH domain. Hydrolysis of plasma membrane PI(4,5)P2 releases E345K GRP1 PH domain into the cytoplasm and the efficiency of this release increases when target Arf6 binding is disrupted. Overall, the findings provide strong support for the sentry glutamate hypothesis and suggest that the GRP1 E345K mutation will be linked to changes in cell physiology and human pathologies, as demonstrated for AKT1 E17K (1, 3). Analysis of available PH domain structures suggests that a lone glutamate residue (or, in some cases an aspartate) is a common, perhaps ubiquitous, feature of PI(3,4,5)P3-specific binding

  14. Pre-clinical evaluation of eight DOTA coupled gastrin-releasing peptide receptor (GRP-R) ligands for in vivo targeting of receptor-expressing tumors.

    Science.gov (United States)

    Accardo, Antonella; Galli, Filippo; Mansi, Rosalba; Del Pozzo, Luigi; Aurilio, Michela; Morisco, Anna; Ringhieri, Paola; Signore, Alberto; Morelli, Giancarlo; Aloj, Luigi

    2016-12-01

    Overexpression of the gastrin-releasing peptide receptor (GRP-R) has been documented in several human neoplasms such as breast, prostate, and ovarian cancer. There is growing interest in developing radiolabeled peptide-based ligands toward these receptors for the purpose of in vivo imaging and radionuclide therapy of GRP-R-overexpressing tumors. A number of different peptide sequences, isotopes, and labeling methods have been proposed for this purpose. The aim of this work is to perform a direct side-by-side comparison of different GRP-R binding peptides utilizing a single labeling strategy to identify the most suitable peptide sequence. Solid-phase synthesis of eight derivatives (BN1-8) designed based on literature analysis was carried out. Peptides were coupled to the DOTA chelator through a PEG4 spacer at the N-terminus. Derivatives were characterized for serum stability, binding affinity on PC-3 human prostate cancer cells, biodistribution in tumor-bearing mice, and gamma camera imaging at 1, 6, and 24 h after injection. Serum stability was quite variable among the different compounds with half-lives ranging from 16 to 400 min at 37 °C. All compounds tested showed K d values in the nanomolar range with the exception of BN3 that showed no binding. Biodistribution and imaging studies carried out for compounds BN1, BN4, BN7, and BN8 showed targeting of the GRP-R-positive tumors and the pancreas. The BN8 compound (DOTA-PEG-DPhe-Gln-Trp-Ala-Val-NMeGly-His-Sta-Leu-NH2) showed high affinity, the longest serum stability, and the highest target-to-background ratios in biodistribution and imaging experiments among the compounds tested. Our results indicate that the NMeGly for Gly substitution and the Sta-Leu substitution at the C-terminus confer high serum stability while maintaining high receptor affinity, resulting in biodistribution properties that outperform those of the other peptides.

  15. Densidade radicular de progênies de pupunheira em função de adubação NPK Root density of pejibaye progenies as a function of NPK fertilization

    Directory of Open Access Journals (Sweden)

    Marilene L. A. Bovi

    1999-11-01

    Full Text Available O uso eficiente de fertilizantes requer o conhecimento de seus efeitos tanto na biomassa aérea quanto na radicular, permitindo que se identifique uma adubação adequada do ponto de vista de uma melhor partição entre ambas. A massa de raízes secas por volume de solo vem sendo utilizada, principalmente em espécies perenes, como subsídio para estimativas de biomassa radicular. Com esse objetivo, foram estudados os efeitos da adubação NPK na densidade radicular de três progênies de pupunheira (Bactris gasipaes Kunth, cultivadas em um Aluvial álico (corrigido por meio de calagem em Ubatuba, SP (clima "Cfa", durante o ano de 1993. O delineamento foi um fatorial fracionado, composto por quatro doses crescentes de nitrogênio (0 a 400 kg/ha/ano de N, fósforo (0 a 200 kg/ha/ano de P2O5 e potássio (0 a 200 kg/ha/ano de K2O, em experimento integrado (progênies e adubação. As amostras foram coletadas por ocasião da primeira colheita de palmito (outubro de 1993, quando as plantas (cultivadas no espaçamento de 2 x 1 m tinham cerca de dois anos de campo. Utilizou-se o método do trado, coletando-se, por parcela experimental, duas amostras na linha e duas na entrelinha. Houve diferenças entre genótipos (GE, com as progênies 2 e 3 apresentando, em média, maior densidade radicular que a progênie 1 (5,15 e 6,20 contra 2,61 g/dm³, respectivamente. A interação GE x PO (posição da amostra foi significativa, sendo que para as progênies 1 e 2 a maior densidade radicular foi obtida na linha (3,30 e 6,60 g/dm³ na linha, contra 1,92 e 4,70 g/dm³ na entrelinha, respectivamente, enquanto para a 3 a ordem foi inversa (5,57 g/dm³ na linha e 6,84 g/dm³ na entrelinha. Doses crescentes de potássio (K apresentaram efeitos lineares positivos e significativos (R² =0,93 a 0,97 na densidade radicular das três progênies, com acréscimos entre dose mínima e máxima variando de 18 a 28%. Não houve efeito significativo isolado de P, enquanto doses

  16. Rev-erb beta regulates the Srebp-1c promoter and mRNA expression in skeletal muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Ramakrishnan, Sathiya N.; Lau, Patrick; Crowther, Lisa M. [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia); Cleasby, Mark E. [Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St. Vincent' s Hospital, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 (Australia); Millard, Susan; Leong, Gary M. [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia); Cooney, Gregory J. [Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St. Vincent' s Hospital, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 (Australia); Muscat, George E.O., E-mail: g.muscat@imb.uq.edu.au [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia)

    2009-10-30

    The nuclear hormone receptor, Rev-erb beta operates as a transcriptional silencer. We previously demonstrated that exogenous expression of Rev-erb{beta}{Delta}E in skeletal muscle cells increased Srebp-1c mRNA expression. We validated these in vitro observations by injection of an expression vector driving Rev-erb{beta}{Delta}E expression into mouse tibialis muscle that resulted in increased Srebp-1c mRNA expression. Paradoxically, Rev-erb{beta} siRNA expression in skeletal muscle cells repressed Srebp-1c expression, and indicated that Rev-erb{beta} expression was necessary for Srebp-1c expression. ChIP analysis demonstrated that Rev-erb{beta} was recruited to the Srebp-1c promoter. Moreover, Rev-erb{beta} trans-activated the Srebp-1c promoter, in contrast, Rev-erb{beta} efficiently repressed the Rev-erb{alpha} promoter, a previously characterized target gene. Finally, treatment with the Rev-erb agonist (hemin) (i) increased the trans-activation of the Srebp-1c promoter by Rev-erb{beta}; and (ii) increased Rev-erb{beta} and Srebp-1c mRNA expression. These data suggest that Rev-erb{beta} has the potential to activate gene expression, and is a positive regulator of Srebp-1c, a regulator of lipogenesis.

  17. Parâmetros genéticos em progênies de polinização livre de acerola Genetic parameters in open pollination progenies of the acerola

    Directory of Open Access Journals (Sweden)

    João Rodrigues de Paiva

    1999-04-01

    Full Text Available A acerola (Malpighia emarginata D.C. é uma cultura que vem despertando grande interesse por parte de consumidores, produtores, industriais e exportadores, dado o seu alto teor de vitamina C. No Brasil, não existem variedades recomendadas para o plantio comercial; por esta razão, os dados de produção são muito variáveis. O presente trabalho visa estimar e interpretar geneticamente a porção de variabilidade existente quanto a alguns caracteres, em uma população de plântulas de aceroleira obtidas de sementes, originalmente selecionadas em plantio comercial no Estado do Ceará. O experimento foi instalado no local definitivo em abril/96, na Estação Experimental da Embrapa-Centro Nacional de Pesquisa de Agroindústria Tropical (CNPAT, no município de Pacajus, CE, sob arranjo experimental de blocos casualizados, com 62 tratamentos, três repetições e quatro plantas por parcelas, no espaçamento de 4 m entre linhas e 3 m entre plantas. Os tratamentos são constituídos por progênies de polinização livre. No primeiro ano de idade das plantas, a maioria das progênies já tinha frutificado, sendo que a proporção de florescimento era menor do que a frutificação no período de avaliação. Pelas estimativas dos coeficientes de herdabilidade dos caracteres, pode-se afirmar que a seleção de progênies resultará em ganhos superiores em relação à seleção de plantas. Os índices b1 e b2, que quantificam a relação da variação genética em face da variação ambiental entre e dentro de progênies, respectivamente, revelam uma condição favorável à seleção dentro de progênies.Acerola (Malpighia emarginata D.C., a typical tropical crop, has shown excellent adaptation in many Brazilian regions. Its high vitamin C content has attracted the interest of consumers, farmers, industries and exporters. Selected materials with desirable characteristics such as high productivity and high fruit quality must be planted to establish new

  18. TEN YEARS OF THE NORTHEASTERN NURSING NETWORK JOURNAL - REV RENE: A DREAM COME TRUE

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    Inacia Sátiro Xavier de Franca

    2011-01-01

    Full Text Available Objetivou-se analisar a memória histórica da Rev Rene ao longo de uma década de sua existência destacando a sua organização, a produção científica e as conquistas alcançadas no concernente a sua indexação nas bases de dados. Estudo fenomenológico cujos dados foram coletados por meio de um formulário, submetidos a associações livres e interpretados em consonância com “A poética do espaço”. Os núcleos foram: Universidade Federal do Ceará (UFC — A casa da Rev Rene; A Rev Rene — ninho — um espaço aberto aos pesquisadores da Saúde e áreas afins; Poética das gavetas do cofre e dos armários — o capital científico da Rev Rene à disposição dos leitores; A Rev Rene — concha — uma homologia poética; A Rev Rene é redonda — Metas e ações para a projeção no cenário nacional e internacional. A Rev Rene transpôs a dimensão do sonho e consolidou-se como um lócus de ciência.

  19. Rev-Erb co-regulates muscle regeneration via tethered interaction with the NF-Y cistrome

    Directory of Open Access Journals (Sweden)

    Ryan D. Welch

    2017-07-01

    Conclusions: Disrupting Rev-Erb activity in injured muscle accelerates regenerative muscle repair/differentiation through transcriptional de-repression of myogenic programs. Rev-Erb, therefore, may be a potent therapeutic target for a myriad of muscular disorders.

  20. Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation

    Directory of Open Access Journals (Sweden)

    Jun Takezawa

    2010-01-01

    Full Text Available When a replicative DNA polymerase stalls upon encountering a lesion on the template strand, it is relieved by other low-processivity polymerase(s, which insert nucleotide(s opposite the lesion, extend by a few nucleotides, and dissociate from the 3′-OH. The replicative polymerase then resumes DNA synthesis. This process, termed translesion replication (TLS or replicative bypass, may involve at least five different polymerases in mammals, although the participating polymerases and their roles have not been entirely characterized. Using siRNAs originally designed and an alkaline sucrose density gradient sedimentation technique, we verified the involvement of several polymerases in ultraviolet (UV light-induced TLS in HeLa cells. First, siRNAs to Rev3 or Rev7 largely abolished UV-TLS, suggesting that these 2 gene products, which comprise Polζ, play a main role in mutagenic TLS. Second, Rev1-targeted siRNA also abrogated UV-TLS, indicating that Rev1 is also indispensable to mutagenic TLS. Third, Polη-targeted siRNA also prevented TLS to a greater extent than our expectations. Forth, although siRNA to Polι had no detectable effect, that to Polκ delayed UV-TLS. To our knowledge, this is the first study reporting apparent evidence for the participation of Polκ in UV-TLS.

  1. The essential Escherichia coli msgB gene, a multicopy suppressor of a temperature-sensitive allele of the heat shock gene grpE, is identical to dapE.

    Science.gov (United States)

    Wu, B; Georgopoulos, C; Ang, D

    1992-08-01

    The grpE gene product is one of three Escherichia coli heat shock proteins (DnaK, DnaJ, and GrpE) that are essential for both bacteriophage lambda DNA replication and bacterial growth at all temperatures. In an effort to determine the role of GrpE and to identify other factors that it may interact with, we isolated multicopy suppressors of the grpE280 point mutation, as judged by their ability to reverse the temperature-sensitive phenotype of grpE280. Here we report the characterization of one of them, designated msgB. The msgB gene maps at approximately 53 min on the E. coli chromosome. The minimal gene possesses an open reading frame that encodes a protein with a predicted size of 41,269 M(r). This open reading frame was confirmed the correct one by direct amino-terminal sequence analysis of the overproduced msgB gene product. Genetic experiments demonstrated that msgB is essential for E. coli growth in the temperature range of 22 to 37 degrees C. Through a sequence homology search, MsgB was shown to be identical to N-succinyl-L-diaminopimelic acid desuccinylase (the dapE gene product), which participates in the diaminopimelic acid-lysine pathway involved in cell wall biosynthesis. Consistent with this finding, the msgB null allele mutant is viable only when the growth medium is supplemented with diaminopimelic acid. These results suggest that GrpE may have a previously unsuspected function(s) in cell wall biosynthesis in E. coli.

  2. Floristic composition and growth of weeds under custard apple (Annona squamosa progenies Composição florística e crescimento de plantas daninhas sob a copa de progênies de fruta-do-conde (Annona squamosa

    Directory of Open Access Journals (Sweden)

    P.S.L. Silva

    2004-12-01

    ção ambiental. Dentre esses métodos, a adoção de cultivares com maior habilidade competitiva com as PD tem despertado atenção internacional. O objetivo do trabalho foi avaliar a composição florística e o crescimento de PD sob a copa de progênies de pinheira irrigadas. Vinte progênies de meias-irmãs, com idade aproximada de três anos, foram avaliadas no delineamento de blocos ao acaso, com cinco repetições e quatro plantas por parcela. Ao redor do caule de cada planta estabeleceu-se um círculo com área de 0,5 m². Nessa área, foram avaliadas a composição e as massas das matérias fresca e seca da parte aérea das PD. Avaliaram-se também os diâmetros do colo e da copa e a área da folha das progênies. Ocorreram 58 espécies de PD. As famílias com maior número de espécies, em ordem decrescente, foram Leguminosae, Convolvulaceae, Euphorbiaceae, Malvaceae e Sterculiaceae. O número de espécies de PD/parcela variou de 6 a 18, porém não houve diferença entre a porcentagem média de diferentes PD sob a copa das progênies. As menores massas de matérias fresca e seca de PD ocorreram nas progênies JG1 e SM8, respectivamente. Não houve diferenças entre progênies quanto a diâmetro do colo e área foliar, mas sob a copa da progênie FE4, que apresentou o maior diâmetro da copa, observou-se um dos menores rendimentos de matéria seca de PD.

  3. Teede REV-2 otsustas börsile minna / Gea Velthut-Sokka

    Index Scriptorium Estoniae

    Velthut-Sokka, Gea, 1977-

    2004-01-01

    Eelmise aasta edukaima ehitusfirma Teede REV-2 ja tema suurinvestori Alta Kapital esindajad põhjendavad börsile mineku otsust. Tabelid ja diagrammid: Veerandi aktsiate omanikuvahetus börsilemineku peaprooviks

  4. REV ÜRÜNLERİN MUHASEBELEŞTİRİLMESİ

    OpenAIRE

    YURT, Eda

    2009-01-01

    Eda, Yurt, Türev Ürünlerin Muhasebelestirilmesi, Tezsiz Yüksek Lisans Dönem Projesi,Danısman: Doç. Dr. Kadir Gürdal, 58 s.BİTİRME PROJESİ ÖZETİProje finansal araçlar olarak türev ürünlerin muhasebelestirilmesine iliskinstandartların incelenmesini amaçlamaktadır. Öncelikle finansal risk tanımlanmıstır.Daha sonra türev piyasaların tarihi anlatılmıs ve islem gördükleri piyasalara göresınıflandırılmıstır. Bu temel açıklamalar sonrasında türev ürünler için UluslararasıMuhasebe Standartları incelen...

  5. The RNA helicase DDX1 is involved in restricted HIV-1 Rev function in human astrocytes

    International Nuclear Information System (INIS)

    Fang Jianhua; Acheampong, Edward; Dave, Rajnish; Wang Fengxiang; Mukhtar, Muhammad; Pomerantz, Roger J.

    2005-01-01

    Productive infection by human immunodeficiency virus type I (HIV-1) in the central nervous system (CNS) involves mainly macrophages and microglial cells. A frequency of less than 10% of human astrocytes is estimated to be infectable with HIV-1. Nonetheless, this relatively low percentage of infected astrocytes, but associated with a large total number of astrocytic cells in the CNS, makes human astrocytes a critical part in the analyses of potential HIV-1 reservoirs in vivo. Investigations in astrocytic cell lines and primary human fetal astrocytes revealed that limited HIV-1 replication in these cells resulted from low-level viral entry, transcription, viral protein processing, and virion maturation. Of note, a low ratio of unspliced versus spliced HIV-1-specific RNA was also investigated, as Rev appeared to act aberrantly in astrocytes, via loss of nuclear and/or nucleolar localization and diminished Rev-mediated function. Host cellular machinery enabling Rev function has become critical for elucidation of diminished Rev activity, especially for those factors leading to RNA metabolism. We have recently identified a DEAD-box protein, DDX1, as a Rev cellular co-factor and now have explored its potential importance in astrocytes. Cells were infected with HIV-1 pseudotyped with envelope glycoproteins of amphotropic murine leukemia viruses (MLV). Semi-quantitative reverse transcriptase-polymerase chain reactions (RT-PCR) for unspliced, singly-spliced, and multiply-spliced RNA clearly showed a lower ratio of unspliced/singly-spliced over multiply-spliced HIV-1-specific RNA in human astrocytes as compared to Rev-permissive, non-glial control cells. As well, the cellular localization of Rev in astrocytes was cytoplasmically dominant as compared to that of Rev-permissive, non-glial controls. This endogenous level of DDX1 expression in astrocytes was demonstrated directly to lead to a shift of Rev sub-cellular distribution dominance from nuclear and/or nucleolar to

  6. Seleção de progênies de meio-irmãos de cenoura baseada em características de sementes Selection of carrot progenies based on seed characteristics

    Directory of Open Access Journals (Sweden)

    Jairo V. Vieira

    2005-03-01

    Full Text Available A menor germinação e emergência de plântulas em campo de cenoura obtida nas sementes de cultivares nacionais em relação às importadas, aumenta o custo de produção além de impossibilitar a otimização do estande final através do emprego de semeadeiras de precisão. Setenta e quatro progênies de meio-irmãos oriundas de uma população de cenoura tipo Brasília foram avaliadas quanto a germinação, vigor e peso de sementes com o objetivo de verificar a variabilidade e as relações genéticas entre os referidos caracteres. Evidenciou-se a existência de diferenças significativas entre as progênies de meio-irmãos em relação a todos os caracteres avaliados. Os altos valores de herdabilidade e variabilidade genética entre progênies para os caracteres avaliados sugerem que métodos de melhoramento simples combinados com métodos de seleção tendo por base a teoria de índice podem ser aplicados visando a melhoria da qualidade fisiológica das sementes de cenoura.Low seed germination and stand establishment of carrot seeds from Brazilian cultivars generally increase the production cost and impairs the use of precision seeders. Germination, vigor and seed weight of 74 progenies from a population of carrot cv. Brasilia were evaluated to verify the variability and the genetic relations among these three characteristics. Significant differences were observed among the progenies in relation to all characteristics. High values of heritability and genetic variability among progenies suggests that simple breeding methods combined with index selection methods may be applied during breeding programs in order to increase the physiological seed quality of carrot.

  7. Pharmacological Targeting the REV-ERBs in Sleep/Wake Regulation

    Science.gov (United States)

    Amador, Ariadna; Huitron-Resendiz, Salvador; Roberts, Amanda J.; Kamenecka, Theodore M.; Solt, Laura A.; Burris, Thomas P.

    2016-01-01

    The circadian clock maintains appropriate timing for a wide range of behaviors and physiological processes. Circadian behaviors such as sleep and wakefulness are intrinsically dependent on the precise oscillation of the endogenous molecular machinery that regulates the circadian clock. The identical core clock machinery regulates myriad endocrine and metabolic functions providing a link between sleep and metabolic health. The REV-ERBs (REV-ERBα and REV-ERBβ) are nuclear receptors that are key regulators of the molecular clock and have been successfully targeted using small molecule ligands. Recent studies in mice suggest that REV-ERB-specific synthetic agonists modulate metabolic activity as well as alter sleep architecture, inducing wakefulness during the light period. Therefore, these small molecules represent unique tools to extensively study REV-ERB regulation of sleep and wakefulness. In these studies, our aim was to further investigate the therapeutic potential of targeting the REV-ERBs for regulation of sleep by characterizing efficacy, and optimal dosing time of the REV-ERB agonist SR9009 using electroencephalographic (EEG) recordings. Applying different experimental paradigms in mice, our studies establish that SR9009 does not lose efficacy when administered more than once a day, nor does tolerance develop when administered once a day over a three-day dosing regimen. Moreover, through use of a time response paradigm, we determined that although there is an optimal time for administration of SR9009 in terms of maximal efficacy, there is a 12-hour window in which SR9009 elicited a response. Our studies indicate that the REV-ERBs are potential therapeutic targets for treating sleep problems as those encountered as a consequence of shift work or jet lag. PMID:27603791

  8. Offshore wind farms in the local environment - an examination at Horns Rev Offshore Wind Farm. Background report; Havvindmoeller i lokalomraadet - en undersoegelse ved Horns Rev Havmoellepark. Baggrundsrapport

    Energy Technology Data Exchange (ETDEWEB)

    Kuehn, Susanne

    2005-07-01

    Horns Rev Offshore Wind Farm was built in 2002. A presentation is made of a sociological, qualitative survey on the local community's reception of the offshore wind farm. The survey aims at identifying attitudes towards the farm before and after the construction, with a view to identifying possible changes in attitudes, and explain the reasons for these (ml)

  9. Nullbasic, a potent anti-HIV tat mutant, induces CRM1-dependent disruption of HIV rev trafficking.

    Directory of Open Access Journals (Sweden)

    Min-Hsuan Lin

    Full Text Available Nullbasic, a mutant of the HIV-1 Tat protein, has anti-HIV-1 activity through mechanisms that include inhibition of Rev function and redistribution of the HIV-1 Rev protein from the nucleolus to the nucleoplasm and cytoplasm. Here we investigate the mechanism of this effect for the first time, establishing that redistribution of Rev by Nullbasic is not due to direct interaction between the two proteins. Rather, Nullbasic affects subcellular localization of cellular proteins that regulate Rev trafficking. In particular, Nullbasic induced redistribution of exportin 1 (CRM1, nucleophosmin (B23 and nucleolin (C23 from the nucleolus to the nucleus when Rev was coexpressed, but never in its absence. Inhibition of the Rev:CRM1 interaction by leptomycin B or a non-interacting RevM10 mutant completely blocked redistribution of Rev by Nullbasic. Finally, Nullbasic did not inhibit importin β- or transportin 1-mediated nuclear import, suggesting that cytoplasmic accumulation of Rev was due to increased export by CRM1. Overall, our data support the conclusion that CRM1-dependent subcellular redistribution of Rev from the nucleolus by Nullbasic is not through general perturbation of either nuclear import or export. Rather, Nullbasic appears to interact with and disrupt specific components of a Rev trafficking complex required for its nucleocytoplasmic shuttling and, in particular, its nucleolar accumulation.

  10. Quantitative genetic analysis of methylxanthines and phenolic compounds in mate progenies Análise genética quantitativa de metilxantinas e compostos fenólicos em progênies de erva-mate

    Directory of Open Access Journals (Sweden)

    Euclides Lara Cardozo Junior

    2010-02-01

    Full Text Available The objective of this work was to determine the contents of methylxanthines, caffeine and theobromine, and phenolic compounds, chlorogenic and caffeic acids, in 51 mate progenies (half-sib families and estimate the heritability of genetic parameters. Mate progenies were from five Brazilian municipalities: Pinhão, Ivaí, Barão de Cotegipe, Quedas do Iguaçu, and Cascavel. The progenies were grown in the Ivaí locality. The contents of the compounds were obtained by high performance liquid chromatography (HPLC. The estimation of genetic parameters by the restricted maximum likelihood (REML and the prediction of genotypic values via best linear unbiased prediction (BLUP were obtained by the Selegen - REML/BLUP software. Caffeine (0.248-1.663% and theobromine (0.106-0.807% contents were significantly different (p0.5. The two different progeny groups determined for chlorogenic (1.365-2.281% and caffeic (0.027-0.037% acid contents were not significantly different (pO objetivo deste trabalho foi determinar o teor de metilxantinas, cafeína e teobromina, e de compostos fenólicos, ácido clorogênico e ácido cafeico, em 51 progênies de erva-mate e estimar componentes de variância e herdabilidade. As progênies de erva-mate eram oriundas de cinco municipios brasileiros: Pinhão, Ivaí, Barão do Cotegipe, Quedas do Iguaçu e Cascavel. Essas progênies foram cultivadas na localidade de Ivaí. O conteúdo dos compostos foi obtido por cromatografia líquida de alta eficiência (CLAE. Na estimativa dos componentes da variância e dos parâmetros genotípicos, utilizou-se a metodologia de modelos mistos para a obtenção da melhor predição linear não viciada (BLUP dos efeitos genotípicos e o processo da máxima verossimilhança restrita (REML, processados pelo programa Selegen - REML/BLUP. Os conteúdos de cafeína (0,248-1,663 % e teobromina (0,106-0.807% foram significativamente (p0,5. Foram determinados dois diferentes grupos de progênie para

  11. Melhoramento do cafeeiro: XLII. Produtividade de progênies derivadas de hibridação dos cultivares Laurina e Mundo Novo Coffee breeding: XLII. Yield of progenies from crosses of Laurina and Mundo Novo cultivars of Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    Alcides Carvalho

    1988-01-01

    Full Text Available O cultivar Laurina de Coffea arabica L. caracteriza-se pelo pequeno porte, folhas de dimensões reduzidas, frutos afilados na base, sementes pequenas e afiladas, pequeno rendimento e reduzida produção. Apresenta, no entanto, bebida de boa qualidade e baixo teor de cafeína nas sementes. Suas principais características são controladas pela ação de um par de alelos recessivos lrlr, de acentuado efeito pleiotrópico. Devido ao atual interesse do comércio por produto de baixo teor de cafeína, iniciaram-se pesquisas tendo em vista principalmente aumentar a produtividade do 'Laurina'. Para esse fim, realizaram-se numerosas hibridações de cafeeiros do 'Laurina' com os do 'Mundo Novo' (Coffea arabica e, posteriormente, retrocruzamentos com o 'Mundo Novo'. Estudaram-se as progênies F2 e retrocruzamentos com o 'Mundo Novo' (RC em Campinas, em um experimento, anotando-se as produções por oito anos consecutivos. Separaram-se algumas progênies F2 em dois grupos, antes do plantio: normais (LrLr,Lrlr e laurina (Irlr. Como testemunhas, usaram-se progênies do 'Mundo Novo' e 'Catuaí Amarelo' de C. arabica. O conjunto de plantas F2 do grupo laurina e os retrocruzamentos tiveram produção média maior do que as plantas F2 normais, porém menor do que as testemunhas. Alguns retrocruzamentos e progênies F2 apresentaram plantas com razoável produtividade, indicando que, através de retrocruzamentos com o 'Mundo Novo', podem-se obter novos tipos comerciais com as características morfológicas do 'Laurina'. Fizeram-se considerações sobre a melhor capacidade de combinação do 'Laurina' com algumas seleções do 'Mundo Novo'.The Laurina cultivars of Coffea arabica L. has a reduced plant size, small leaves, small and pointed seeds and low yield capacity. However the seeds have a good cup quality and the desirable characteristic of low caffeine content The Laurina phenotype is supposed to be controlled by a pair of recessive alleles lrlr, with

  12. Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export function

    Directory of Open Access Journals (Sweden)

    St-Laurent Georges

    2006-11-01

    Full Text Available Abstract Background The HIV Rev protein is known to facilitate export of incompletely spliced and unspliced viral transcripts to the cytoplasm, a necessary step in virus life cycle. The Rev-mediated nucleo-cytoplasmic transport of nascent viral transcripts, dependents on interaction of Rev with the RRE RNA structural element present in the target RNAs. The C-terminal variant of dsRNA-binding nuclear protein 90 (NF90ctv has been shown to markedly attenuate viral replication in stably transduced HIV-1 target cell line. Here we examined a mechanism of interference of viral life cycle involving Rev-NF90ctv interaction. Results Since Rev:RRE complex formations depend on protein:RNA and protein:protein interactions, we investigated whether the expression of NF90ctv might interfere with Rev-mediated export of RRE-containing transcripts. When HeLa cells expressed both NF90ctv and Rev protein, we observed that NF90ctv inhibited the Rev-mediated RNA transport. In particular, three regions of NF90ctv protein are involved in blocking Rev function. Moreover, interaction of NF90ctv with the RRE RNA resulted in the expression of a reporter protein coding sequences linked to the RRE structure. Moreover, Rev influenced the subcellular localization of NF90ctv, and this process is leptomycin B sensitive. Conclusion The dsRNA binding protein, NF90ctv competes with HIV Rev function at two levels, by competitive protein:protein interaction involving Rev binding to specific domains of NF90ctv, as well as by its binding to the RRE-RNA structure. Our results are consistent with a model of Rev-mediated HIV-1 RNA export that envisions Rev-multimerization, a process interrupted by NF90ctv.

  13. Parâmetros genéticos em progênies de polinização aberta de Cordia trichotoma (Vell. ex Steud. Genetic parameters in open-pollinated families of Cordia trichotoma (Vell. ex Steud.

    Directory of Open Access Journals (Sweden)

    Miguel Luiz Menezes FREITAS

    2006-12-01

    Full Text Available O objetivo deste trabalho foi estimarparâmetros genéticos para caracteres de crescimento eforma, em um teste de 22 progênies de polinizaçãoaberta de Cordia trichotoma, procedentes deBauru–SP, aos 19 anos de idade, implantado emLuiz Antonio–SP. A análise de variância detectoudiferenças significativas entre progênies para ocaráter DAP, forma e volume. As diferenças entrea maior e a menor média de crescimento em DAP,altura, forma, volume real e sobrevivência entreprogênies foram, respectivamente, de 87,3%;42,4%; 77,6%; 156,6% e 61,2%. O coeficiente devariação genética para DAP e volume foi alto(> 10%. Os valores dos coeficientes de herdabilidade,em nível de plantas individuais ( 2i h e dentro deprogênies ( 2d h , foram baixos ( 10%.The values of individual narrow sense ( 2i h andwithin families ( 2d h heritability coefficients werelow (< 0.2 for height and stem form traits, butthese values were high for DBH and volume traits(varying from 0.29 to 0.65, indicating possibilitiesto obtain genetic gains on selection at traits andbetween families. The estimates of meanheritability ( 2mh were low for plant height (0.12,but high for the other traits, varying from 0.46(form to 0.77 (DBH and indicating the possibilityto obtain considerable gains on selection of thebest families. The expected genetic gains withselection within families to DBH, height, stemform and volume were 17.43%, 1.02%, 2.66%, and18.72%, respectively. The expected were predictedfor Cordia trichotoma plantations 19 years old,growing under similar environmental conditions tothe Luiz Antonio, SP.

  14. REV-ERBalpha participates in circadian SREBP signaling and bile acid homeostasis.

    Directory of Open Access Journals (Sweden)

    Gwendal Le Martelot

    2009-09-01

    Full Text Available In mammals, many aspects of behavior and physiology, and in particular cellular metabolism, are coordinated by the circadian timing system. Molecular clocks are thought to rely on negative feedback loops in clock gene expression that engender oscillations in the accumulation of transcriptional regulatory proteins, such as the orphan receptor REV-ERBalpha. Circadian transcription factors then drive daily rhythms in the expression of clock-controlled output genes, for example genes encoding enzymes and regulators of cellular metabolism. To gain insight into clock output functions of REV-ERBalpha, we carried out genome-wide transcriptome profiling experiments with liver RNA from wild-type mice, Rev-erbalpha knock-out mice, or REV-ERBalpha overexpressing mice. On the basis of these genetic loss- and gain-of-function experiments, we concluded that REV-ERBalpha participates in the circadian modulation of sterol regulatory element-binding protein (SREBP activity, and thereby in the daily expression of SREBP target genes involved in cholesterol and lipid metabolism. This control is exerted via the cyclic transcription of Insig2, encoding a trans-membrane protein that sequesters SREBP proteins to the endoplasmic reticulum membranes and thereby interferes with the proteolytic activation of SREBPs in Golgi membranes. REV-ERBalpha also participates in the cyclic expression of cholesterol-7alpha-hydroxylase (CYP7A1, the rate-limiting enzyme in converting cholesterol to bile acids. Our findings suggest that this control acts via the stimulation of LXR nuclear receptors by cyclically produced oxysterols. In conclusion, our study suggests that rhythmic cholesterol and bile acid metabolism is not just driven by alternating feeding-fasting cycles, but also by REV-ERBalpha, a component of the circadian clockwork circuitry.

  15. Incorporação da dependência espacial na análise de um experimento de avaliação de progênies de milho quanto à resistência à ferrugem comum Spatial dependency analysis of a maize progeny to evaluation the common rust resistance

    Directory of Open Access Journals (Sweden)

    Heyder Diniz Silva

    2004-10-01

    Full Text Available Fenômenos naturais, principalmente aqueles observados na agricultura, tais como produtividade de grãos e incidência de doenças, apresentam-se espacialmente autocorrelacionados. Conduziu-se este trabalho com o objetivo de avaliar a existência de dependência espacial entre as notas, relativas à resistência de progênies de milho à ferrugem comum e seus efeitos sobre a seleção de progênies. O delineamento experimental utilizado foi em blocos ao acaso com três repetições e 100 tratamentos (98 progênies F3 e duas testemuhas. Na análise dos dados, considerou-se o modelo com erros independentes e com erros espacialmente autocorrelacionados. Verificou-se que o modelo com erros espacialmente correlacionados ajustou-se melhor aos dados. O efeito da dependência espacial foi altamente significativo, com alcance de 1,36 m. O número de progênies selecionadas ao se considerar a dependência espacial caiu de 23 para 11, e essas apresentaram um padrão de dispersão no campo bem mais condizente com o esperado.The aim of this work was to evaluate the spatial dependency among the resistance scores of 98 maize progenies to the common rust (Puccinia sorghi and its effects over the selection process. A randomized block design with 100 treatments (98 F2 progênies and two checks was used. Models with independents and spacialy autocorrelated error were taken in account for the data analysis. The experiment showed a high spacial dependence. 23 progenies had been selected with the unadjusted means, and only 11 progenies showed the status of resistant, when the spacial dependence was take into account.

  16. The insulin/IGF signaling regulators cytohesin/GRP-1 and PIP5K/PPK-1 modulate susceptibility to excitotoxicity in C. elegans.

    Directory of Open Access Journals (Sweden)

    Nazila Tehrani

    Full Text Available During ischemic stroke, malfunction of excitatory amino acid transporters and reduced synaptic clearance causes accumulation of Glutamate (Glu and excessive stimulation of postsynaptic neurons, which can lead to their degeneration by excitotoxicity. The balance between cell death-promoting (neurotoxic and survival-promoting (neuroprotective signaling cascades determines the fate of neurons exposed to the excitotoxic insult. The evolutionary conserved Insulin/IGF Signaling (IIS cascade can participate in this balance, as it controls cell stress resistance in nematodes and mammals. Blocking the IIS cascade allows the transcription factor FoxO3/DAF-16 to accumulate in the nucleus and activate a transcriptional program that protects cells from a range of insults. We study the effect of IIS cascade on neurodegeneration in a C. elegans model of excitotoxicity, where a mutation in a central Glu transporter (glt-3 in a sensitizing background causes Glu-Receptor -dependent neuronal necrosis. We expand our studies on the role of the IIS cascade in determining susceptibility to excitotoxic necrosis by either blocking IIS at the level of PI3K/AGE-1 or stimulating it by removing the inhibitory effect of ZFP-1 on the expression of PDK-1. We further show that the components of the Cytohesin/GRP-1, Arf, and PIP5K/PPK-1 complex, known to regulate PIP2 production and the IIS cascade, modulate nematode excitotoxicity: mutations that are expected to reduce the complex's ability to produce PIP2 and inhibit the IIS cascade protect from excitotoxicity, while overstimulation of PIP2 production enhances neurodegeneration. Our observations therefore affirm the importance of the IIS cascade in determining the susceptibility to necrotic neurodegeneration in nematode excitotoxicity, and demonstrate the ability of Cytohesin/GRP-1, Arf, and PIP5K/PPK-1 complex to modulate neuroprotection.

  17. Contributions of individual domains to function of the HIV-1 Rev response element.

    Science.gov (United States)

    O'Carroll, Ina P; Thappeta, Yashna; Fan, Lixin; Ramirez-Valdez, Edric A; Smith, Sean; Wang, Yun-Xing; Rein, Alan

    2017-08-16

    The HIV-1 Rev response element (RRE) is a 351-base element in unspliced and partially spliced viral RNA; binding of the RRE by the viral Rev protein induces nuclear export of RRE-containing RNAs, as required for virus replication. It contains one long, imperfect double helix (domain I), one branched domain (domain II) containing a high-affinity Rev-binding site, and two or three additional domains. We previously reported that the RRE assumes an "A" shape in solution and suggested that the location of the Rev binding sites in domains I and II, opposite each other on the two legs of the A, is optimal for Rev binding and explains Rev's specificity for RRE-containing RNAs. Using SAXS and a quantitative functional assay, we have now analyzed a panel of RRE mutants. All the results support the essential role of the A shape for RRE function. Moreover, they suggest that the distal portion of domain I and the three crowning domains all contribute to the maintenance of the A shape. Domains I and II are necessary and sufficient for substantial RRE function, provided they are joined by a flexible linker that allows the two domains to face each other. IMPORTANCE Retroviral replication requires that some of the viral RNAs transcribed in the cell nucleus be exported to the cytoplasm without being spliced. To achieve this, HIV-1 encodes a protein, Rev, which binds to a complex, highly structured element within viral RNA, the Rev Response Element (RRE), and escorts RRE-containing RNAs from the nucleus. We previously reported that the RRE is "A"-shaped and suggested that this architecture, with the 2 legs opposite one another, can explain the specificity of Rev for the RRE. We have analyzed the functional contributions of individual RRE domains, and now report that several domains contribute, with some redundancy, to maintenance of the overall RRE shape. The data strongly support the hypothesis that the opposed placement of the 2 legs is essential for RRE function. Copyright © 2017

  18. B23/nucleophosmin interacts with bovine immunodeficiency virus Rev protein and facilitates viral replication.

    Science.gov (United States)

    Passos-Castilho, Ana Maria; Marchand, Claude; Archambault, Denis

    2018-02-01

    The bovine immunodeficiency virus (BIV) Rev shuttling protein contains nuclear/nucleolar localization signals and nuclear import/export mechanisms that are novel among lentivirus Rev proteins. Several viral proteins localize to the nucleolus, which may play a role in processes that are essential to the outcome of viral replication. Although BIV Rev localizes to the nucleoli of transfected/infected cells and colocalizes with one of its major proteins, nucleophosmin (NPM1, also known as B23), the role of the nucleolus and B23 in BIV replication remains to be determined. Here, we demonstrate for the first time that BIV Rev interacts with nucleolar phosphoprotein B23 in cells. Using small interfering RNA (siRNA) technology, we show that depletion of B23 expression inhibits virus production by BIV-infected cells, indicating that B23 plays an important role in BIV replication. The interaction between Rev and B23 may represent a potential new target for the development of antiviral drugs against lentiviruses. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. SEM-REV: A sea test site for Marine Energy Converter

    International Nuclear Information System (INIS)

    Berhault, Christian; Le Crom, Izan; Le Bihan, Gerard

    2015-01-01

    Thanks to a main funding of Region des Pays de la Loire, the sea test site SEM-REV has been developed by Ecole Centrale de Nantes since 2007 to test both Wave Energy Converters and Floating Wind Turbine in real sea conditions. The sea test site is equipped with a set of oceanographic sensors and with an electric cable of 8 MW, connected to the French grid. The project is located close to Le Croisic, Western coast of France. SEM-REV is one of the main test facilities operated by Ecole Centrale de Nantes to support MRE technologies development. After presenting the initial motivations of the SEM-REV development, the paper describes, in parts 1 and 2, the complete administrative and technical processes that were followed to reach SEM-REV commissioning in 2014. The third part is focused on the exploitation process, including technical and contractual specifications imposed to the MRE developers for installation, tests and decommissioning phases. Some words are given also on the R and D projects using the SEM-REV in-situ monitoring system: prediction of environmental conditions, bio-fooling, acoustic impact. Even if operational phase is not started, expected extensions of the tests site are listed. (authors)

  20. Ultraviolet-induced reversion of cyc1 alleles in radiation-sensitive strains of yeast. III. rev 3 mutant strains

    International Nuclear Information System (INIS)

    Lawrence, C.W.; Crhistensen, R.B.

    1979-01-01

    The role of rev3 gene function in uv-induced mutagenesis in the yeast Saccharomyces cerevisiae has been examined by determining the reversion of 12 well-defined cyc1 mutations in diploid strains homozygous for the rev3-1 or rev3-3 allale. The 12 cyc1 alleles include one ochre, one amber, four initiation, two proline missense, and four frameshift mutations. We find that the rev3 mutations reduce the frequency of UV-induced reversion of all of the cyc1 alleles, though different classes of alleles respond to a different extent. These results imply that the rev3 gene function is required for the production of a wide variety of mutational events, though probably not all, and show that each of the three rev loci have different mutational phenotypes. Such diverse phenotypes are not predicted by the unitary model for bacterial mutagenes, suggesting that this is at best an incomplete description of eukaryotic mutagenesis

  1. Innocuity and immune response to Brucella melitensis Rev.1 vaccine in camels (Camelus dromedarius

    Directory of Open Access Journals (Sweden)

    A. Benkirane

    2014-10-01

    Full Text Available A field trial was conducted in a camel brucellosis-free herd to evaluate antibody response to the Brucella melitensis Rev.1 vaccine in camels and assess shedding of the vaccine strain in milk. Twenty eight camels were divided into four groups according to their age and vaccination route. Groups A (n=3 and B (n=3 consisted of non-pregnant lactating female camels, vaccinated through subcutaneous and conjunctival routes, respectively. Groups C (n=10 consisted of 8-11 months old calves vaccinated through conjunctival route. The rest of the herd (n=12 composed of female and young camels were not vaccinated and were considered as the control group. Each animal from groups A, B and C was given the recommended dose of 2 x 109 colony forming units of Rev.1 vaccine irrespective of age or route of vaccination. Blood samples were collected from all the animals at the time of vaccination and at weekly, bi-weekly and monthly interval until 32 weeks post vaccination and from controls at weeks 8 and 24. The serological tests used were modified Rose Bengal Test, sero-agglutination test, and an indirect Enzyme Linked Immunosorbent Assay. Milk samples were collected from all vaccinated female camels and tested for the presence of Rev.1 vaccine strain. Most vaccinated animals started to show an antibody response at week 2 and remained positive until week 16. By week 20 post-vaccination all animals in the three groups were tested negative for Brucella antibodies. Bacteriological analysis of milk samples did not allow any isolation of Brucella melitensis. All samples were found Brucella negative in PCR analysis. The results of this study indicate that the Rev.1 vaccine induces seroconversion in camels. Rev.1 vaccine strain is not excreted in the milk of camels. These findings are promising as to the safe use of the Rev.1 vaccine in camels.

  2. Seleção para a resistência à ferrugem em progênies das cultivares de café IPR 99 e IPR 107 Selection for resistance to rust in progenies of coffee cultivars IPR 99 and IPR 107

    Directory of Open Access Journals (Sweden)

    Gustavo Hiroshi Sera

    2010-01-01

    Full Text Available As cultivares de café IPR 99 ("Sarchimor" e IPR 107 ('IAPAR 59' x 'Mundo Novo IAC 376-4' eram no passado resistentes à ferrugem (Hemileia vastatrix. Os objetivos deste estudo foram: a Identificar progênies dessas duas cultivares com resistência à ferrugem; b Identificar progênies com resistência incompleta; c Verificar a eficiência dos cruzamentos testes na seleção dessas cultivares. A avaliação da resistência em campo foi realizada em cafeeiros adultos expostos à população local de raças presentes no IAPAR. Foram avaliados 23 cruzamentos testes com progênies F3 de 'IPR 107' e 5 com progênies F4 de 'IPR 99'. Além disso, foram avaliadas 11 progênies F4 de 'IPR 107' e 5 progênies F5 de 'IPR 99', provenientes de autofecundação. Várias progênies das cultivares IPR 99 e 107 apresentaram alta freqüência de plantas com resistência completa e são portadoras de mais genes de resistência não quebrados pelas raças de ferrugem. Progênies das cultivares IPR 99 e IPR 107 com a resistência quebrada apresentaram resistência incompleta à população local de raças. Cruzamentos testes foram eficientes na seleção de progênies de café com mais genes de resistência não quebrados.All plants of the coffee cultivars IPR 99 ("Sarchimor" and IPR 107 ('IAPAR 59' x 'Mundo Novo IAC 376-4' were resistant to rust (Hemileia vastatrix in the past. Currently, susceptible plants were observed in the two cultivars due to the breakdown of resistance by new rust races. The aims of this study were: a to identify coffee (Coffea arabica L. progenies of the two cultivars with resistance to rust; b to identify progenies with incomplete resistance; c to investigate the efficiency of test-crosses for selection from these cultivars. Evaluation for field resistance was carried out in adult plants subjected to natural infection by the local leaf rust population at IAPAR. Twenty-three test-crosses with F3 progenies of 'IPR 107' and five with F4

  3. Unprecedented NES non-antagonistic inhibitor for nuclear export of Rev from Sida cordifolia.

    Science.gov (United States)

    Tamura, Satoru; Kaneko, Masafumi; Shiomi, Atsushi; Yang, Guang-Ming; Yamaura, Toshiaki; Murakami, Nobutoshi

    2010-03-15

    Bioassay-guided separation from the MeOH extract of the South American medicinal plant Sida cordifolia resulted in isolation of (10E,12Z)-9-hydroxyoctadeca-10,12-dienoic acid (1) as an unprecedented NES non-antagonistic inhibitor for nuclear export of Rev. This mechanism of action was established by competitive experiment by the biotinylated probe derived from leptomycin B, the known NES antagonistic inhibitor. Additionally, structure-activity relationship analysis by use of the synthesized analogs clarified cooperation of several functionalities in the Rev-export inhibitory activity of 1. Copyright 2010 Elsevier Ltd. All rights reserved.

  4. Administrative Circular No. 22B (Rev. 2) - Compensation for hours of long-term shift work

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 22B (Rev. 2) entitled "Compensation for hours of long-term shift work",  approved by the Director-General following discussion in the Standing Concertation Committee meeting on 22 March 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208538.   This revised circular cancels and replaces Administrative Circular No. 22B (Rev. 1) also entitled "Compensation for hours of long-term shift work" of March 2011. This document contains minor changes to reflect the new career structure. This circular will enter into force on 1st September 2016.

  5. Administrative Circular No. 13 (Rev. 4) - Guarantees for representatives of the personnel

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 13 (Rev. 4) entitled "Guarantees for representatives of the personnel", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 22 March 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208527.   This revised circular cancels and replaces Administrative Circular No. 13 (Rev. 3) also entitled "Guarantees for representatives of the personnel" of January 2014. This document contains a single change to reflect the terminology under the new career structure: the term "career path" is replaced by "grade". This circular will enter into force on 1st September 2016.

  6. Empirical investigation of wind farm blockage effects in Horn Rev 1 offshore wind farm

    DEFF Research Database (Denmark)

    Mitraszewski, Karol; Hansen, Kurt Schaldemose; Nygaard, Nicolai

    We present an empirical study of wind farm blockage effects based on Horns Rev 1 SCADA data. The mean inflow non-uniformities in wind speed are analyzed by calculating the mean power outputs of turbines located along the outer edges of the farm for different wind directions, wind speeds and stabi......We present an empirical study of wind farm blockage effects based on Horns Rev 1 SCADA data. The mean inflow non-uniformities in wind speed are analyzed by calculating the mean power outputs of turbines located along the outer edges of the farm for different wind directions, wind speeds...

  7. Investigations of migratory birds during operation of Horns Rev offshore wind farm. Annual status report 2004

    Energy Technology Data Exchange (ETDEWEB)

    Kjaer Christensen, T.; Hounisen, J.P. [NERI, Dept. of Wildlife Ecology and Biodiversity, Roskilde (Denmark)

    2005-07-01

    The aim of the project is to assess the collision risk between birds and wind turbines at the Horns Rev wind farm. The study focused on describing bird movements in relation to the wind farm and to identify the species-specific behavioural responses towards the wind turbines shown by migrating and staging bird species. The study was based on data from spring 2004. The Horns Rev area lies in a region known to be of importance for substantial water bird migration as well as holding internationally important numbers of several wintering and staging water bird species. (au)

  8. Investigations of migratory birds during operation of Horns Rev offshore wind farm. Annual status report 2004

    International Nuclear Information System (INIS)

    Kjaer Christensen, T.; Hounisen, J.P.

    2005-01-01

    The aim of the project is to assess the collision risk between birds and wind turbines at the Horns Rev wind farm. The study focused on describing bird movements in relation to the wind farm and to identify the species-specific behavioural responses towards the wind turbines shown by migrating and staging bird species. The study was based on data from spring 2004. The Horns Rev area lies in a region known to be of importance for substantial water bird migration as well as holding internationally important numbers of several wintering and staging water bird species. (au)

  9. RevTrans: multiple alignment of coding DNA from aligned amino acid sequences

    DEFF Research Database (Denmark)

    Wernersson, Rasmus; Pedersen, Anders Gorm

    2003-01-01

    The simple fact that proteins are built from 20 amino acids while DNA only contains four different bases, means that the 'signal-to-noise ratio' in protein sequence alignments is much better than in alignments of DNA. Besides this information-theoretical advantage, protein alignments also benefit...... proteins. It is therefore preferable to align coding DNA at the amino acid level and it is for this purpose we have constructed the program RevTrans. RevTrans constructs a multiple DNA alignment by: (i) translating the DNA; (ii) aligning the resulting peptide sequences; and (iii) building a multiple DNA...

  10. Administrative Circular No. 11 (Rev. 4) - Categories of members of the personnel

    CERN Multimedia

    HR Department

    2016-01-01

    Administrative Circular No. 11 (Rev. 4) entitled "Categories of members of the personnel", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 29 April 2016, will be available on 1 August 2016 via this following link.    This revised circular cancels and replaces the Administrative Circular No. 11 (Rev. 3) also entitled "Categories of members of the personnel" of September 2014. The main changes concern the status of apprentices and their transfer from the category of employed members of personnel to associated members of personnel. This circular will enter into force on 1 August 2016. Department Head Office

  11. Rev1 Recruits Ung to Switch Regions and Enhances dU Glycosylation for Immunoglobulin Class Switch DNA Recombination

    Directory of Open Access Journals (Sweden)

    Hong Zan

    2012-11-01

    Full Text Available By diversifying the biological effector functions of antibodies, class switch DNA recombination (CSR plays a critical role in the maturation of the immune response. It is initiated by activation-induced cytidine deaminase (AID-mediated deoxycytosine deamination, yielding deoxyuridine (dU, and dU glycosylation by uracil DNA glycosylase (Ung in antibody switch (S region DNA. Here we showed that the translesion DNA synthesis polymerase Rev1 directly interacted with Ung and targeted in an AID-dependent and Ung-independent fashion the S regions undergoing CSR. Rev1−/− Ung+/+ B cells reduced Ung recruitment to S regions, DNA-dU glycosylation, and CSR. Together with an S region spectrum of mutations similar to that of Rev1+/+ Ung−/− B cells, this suggests that Rev1 operates in the same pathway as Ung, as emphasized by further decreased CSR in Rev1−/− Msh2−/− B cells. Rescue of CSR in Rev1−/− B cells by a catalytically inactive Rev1 mutant shows that the important role of Rev1 in CSR is mediated by Rev1’s scaffolding function, not its enzymatic function.

  12. Caracterização fenotípica e genotípica de progênies de pupunheira para palmito Phenotypic and genotypic characterization in pejibayes progenies for palm heart

    Directory of Open Access Journals (Sweden)

    Gilberto Ken-Iti Yokomizo

    2003-01-01

    Full Text Available A pupunheira (Bactris gasipaes H.B.K. é considerada a espécie com maior potencial de produção de palmitos, em substituição aos tradicionais açaí (Euterpe oleracea Mart. e juçara (Euterpe edulis Mart.. Os objetivos deste trabalho foram estimar alguns parâmetros genéticos associados à avaliação de 31 progênies de meios-irmãos de pupunheiras e classificar as melhores progênies pelo teste de médias de Scott-Knott. O delineamento experimental usado foi o de blocos ao acaso com três repetições, com parcelas experimentais de cinco plantas. As avaliações dos caracteres de altura da planta no momento do corte (APC, diâmetro da planta na altura do colo (DPC e peso do palmito líquido (PPL foram realizadas aos 15, 26 e 37 meses após o plantio. A população avaliada apresentou variação genética disponível para seleção e não sofreu efeito de interação com os cortes; o caráter PPL deverá ser testado com maior número de repetições, sendo o caráter mais sensível a efeitos de cortes; a razão entre o coeficiente de variação genética e o coeficiente de variação do erro indica que existem dificuldades nos processos de seleção nos caracteres avaliados; a correlação genotípica entre os caracteres DPC e PPL apresenta-se promissora para facilitar a seleção indireta do caráter PPL pelo caráter DPC. O agrupamento de médias de Scott-Knott classificou as progênies em dois grupos.The pejibaye (Bactris gasipaes H.B.K. is the species with the best potential in supplying the production of palm heart, in substitution to açaí (Euterpe oleracea Mart. and juçara (Euterpe edulis Mart. species, traditionally produced. The objectives of this work were to estimate some genetic parameters associated to the evaluation of 31 pejibayes half-sib progenies and to classify the best progenies, using the Scott-Knott test for means classification. The experimental design was a randomized complete block with three replications, and

  13. Comportamento de progênies oriundas de raças primitivas de algodão herbáceo frente ao ataque do bicudo Behavior of lines from cotton primitive race stocks to attack of the boll weevil

    Directory of Open Access Journals (Sweden)

    Francisco José Correia Farias

    1999-12-01

    Full Text Available Com o objetivo de obter linhagens resistentes ao bicudo-do-algodoeiro (Anthonomus grandis Boheman, a Embrapa-Centro Nacional de Pesquisa de Algodão vem testando progênies oriundas de raças primitivas de algodoeiro herbáceo (Gossypium hirsutum L. originárias do México e da América Central, que apresentam níveis aceitáveis de resistência ao bicudo. Em 1991 e 1992, as progênies em BC1F5 e BC1F6 oriundas das linhagens Texas 277, Texas 326 e Texas 1180, Texas 297, Texas 339, Texas 766 e Texas 1134, foram avaliadas com relação à resistência ao bicudo. O delineamento experimental utilizado foi o de blocos ao acaso, com seis repetições. A unidade experimental foi constituída por duas fileiras de 5 m, sob um espaçamento de 0,75 m x 0,20 m. As parcelas foram infestadas com adultos do bicudo recém-emergidos, a uma taxa de 10.000 adultos/ha. Aos seis dias após a liberação dos adultos, as parcelas foram pulverizadas com Cipermethrim, sendo realizadas em intervalos semanais. Foram procedidas cinco avaliações através da coleta de 33 botões florais ao acaso, por parcela. Os maiores níveis de resistência ao bicudo foram obtidos pelas progênies Texas 326-95-1, Texas 277-87-5, Texas 1180-99-2, Texas 297 e Texas 339, com redução de ataque de 44,0, 41,2, 32,0, 40,4 e 36,4%, respectivamente, em relação à testemunha CNPA 6H.The goal of the boll weevil (Anthonomus grandis Boheman resistance program of Embrapa-Centro Nacional de Pesquisa de Algodão is to obtain cultivars that give 80% suppression when compared to commercial cultivars. The primitive cottons (Gossypium hirsutum L. from Mexico and Central America have been known to show measurable levels of resistance to the boll weevil. In 1991 and 1992, the lines in BC1F5 and BC1F6 from Texas 277, Texas 326, Texas 1180, Texas 297, Texas 339, Texas 766 and Texas 1134 were evaluated to boll weevil resistance. The experiment was carried out at Campina Grande, PB, Brazil. The trial

  14. Avaliação da atividade "in vivo" da fosfatase ácida e do crescimento de progênies de pupunheira cultivadas em duas doses de nitrogênio e fósforo

    Directory of Open Access Journals (Sweden)

    M. L. A. Bovi

    1998-09-01

    Full Text Available O presente trabalho, realizado em janeiro de 1995, visou avaliar a atividade da enzima fosfatase ácida em folhas de quatro progênies de pupunheira (Bactris gasipaes Kunth, cultivadas em duas doses de nitrogênio e duas de fósforo. Para tanto, utilizou-se a porção média da segunda folha mais jovem de perfilhos de palmeiras com quatro anos de idade. As plantas analisadas representam parcelas submetidas a duas doses de nitrogênio (N1 = 0, N2 = 400 kg ha-1 ano-1 de N e duas doses de fósforo (P1 = 0, P2 = 200 kg ha-1 ano-1 de P2O5. A dose de potássio foi 200 kg ha-1 ano-1 de K2O. Análises dos elementos no solo e nas folhas foram efetuadas e correlações foram estimadas entre as características avaliadas e o crescimento e a produção de palmito dos quatro tratamentos. Foram observados valores médios de atividade da fosfatase ácida de 8,35; 4,58; 10,84, e 11,05 µmol h-1 g-1, para os tratamentos N2P2, N2P1, N1P2 e N1P1, respectivamente. Houve diferenças significativas de atividade entre doses de nitrogênio (10,95 e 6,47 µmol h-1 g-1, para N1 e N2, respectivamente e entre progênies (variando de 6,18 (G3 a 10,10 (G1 µmol h-1 g-1, indicando que esses dois fatores devem ser levados em conta em estudos dessa natureza. A atividade da fosfatase ácida apresentou correlação negativa com as características que avaliam o crescimento (biomassa aérea e radicular e a produção de palmito (peso e diâmetro das plantas estudadas, não se correlacionando com os teores de fósforo no solo ou no tecido foliar.

  15. Microminerais complexados a aminoácidos no desempenho reprodutivo de matrizes pesadas e resposta da progênie Complexed trace minerals to amino acids on broiler breeders reproductive performance and response from progeny

    Directory of Open Access Journals (Sweden)

    Ana Carolina Tozzo Guimarães

    2013-06-01

    Full Text Available Este estudo teve como objetivo avaliar o uso de microminerais complexados a aminoácidos na alimentação de matrizes de frango de corte sobre o desempenho, variáveis de incubação e desempenho da progênie. O estudo foi dividido em três experimentos: exp.1. desempenho das matrizes, exp.2. incubação e exp.3. desempenho da progênie. No exp. 1, foram utilizadas 260 aves da linhagem CobbAvian 48 (240 matrizes e 20 machos, avaliando-se produção e massa de ovos, conversão alimentar por massa e por dúzia de ovos, peso e gravidade específica. No exp. 2, determinou-se a taxa de eclosão, eclodibilidade e fertilidade. No exp. 3 avaliou-se o desempenho dos pintinhos nascidos das incubações. No exp. 1, não houve interação entre tratamento e período (P>0,05 para produção de ovos, massa de ovos, conversão alimentar por massa e por dúzia de ovos, peso e gravidade específica dos ovos. Contudo, houve efeito (PThis study aimed to evaluate the use of complexed trace minerals to amino acid in broiler breeder feeding evaluating performance, incubation variables and performance of progeny. The study was divided in three trials: exp. 1. broiler breeders performance, exp. 2. incubation and exp. 3. chickens performance. In the exp. 1 two hundred sixty broilers breeders of lineage Cobb Avian 48 (240 females and 20 males were used. The variables evaluated were egg production and egg mass, feed conversion for egg mass and per dozen eggs, egg weight and specific gravity. In the exp. 2 it was determined rate of hatching, hatchability and fertility. In the exp. 3, it was evaluated the performance of chicks born from incubations. In the exp. 1, there was no interaction between treatment and period (P>0.05 for egg production, egg mass, feed conversion for egg mass and per dozen eggs, egg weight and specific gravity of eggs. However, there was effect (P<0.05 of the period for all variables mentioned above. In the exp. 2, hatching rate, fertility and

  16. Metabolism and apoptotic properties of elevated ceramide in HT29(rev) cells

    NARCIS (Netherlands)

    Veldman, R J; Klappe, K; Hoekstra, D; Kok, J W

    1998-01-01

    Ceramide (Cer) has been implicated in the regulation of apoptosis. In this study, we elevated cellular Cer levels in human colon-carcinoma (HT29(rev)) cells by incubating the cells in the presence of bacterial sphingomyelinase (bSMase) or, alternatively, in the presence of C2-Cer, a short-chain

  17. Material Trends ( From M.Imada, A. Fujimori, and Y. Tokura, Rev ...

    Indian Academy of Sciences (India)

    First page Back Continue Last page Overview Graphics. Material Trends ( From M.Imada, A. Fujimori, and Y. Tokura, Rev. Mod. Phys. 70, 1039 (1998) ). Differences due to changes in cation radius causing bending of Mn-O-Mn bonds. Extreme sensitivity to small changes.

  18. 48 CFR 53.301-18 - SF 18 (Rev. 6/95), Request for Quotations.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false SF 18 (Rev. 6/95), Request for Quotations. 53.301-18 Section 53.301-18 Federal Acquisition Regulations System FEDERAL ACQUISITION... for Quotations. EC01MY91.002 [60 FR 34763, July 3, 1995] ...

  19. Determining the REV for Fracture Rock Mass Based on Seepage Theory

    Directory of Open Access Journals (Sweden)

    Lili Zhang

    2017-01-01

    Full Text Available Seepage problems of the fractured rock mass have always been a heated topic within hydrogeology and engineering geology. The equivalent porous medium model method is the main method in the study of the seepage of the fractured rock mass and its engineering application. The key to the method is to determine a representative elementary volume (REV. The FractureToKarst software, that is, discrete element software, is a main analysis tool in this paper and developed by a number of authors. According to the standard of rock classification established by ISRM, this paper aims to discuss the existence and the size of REV of fractured rock masses with medium tractility and provide a general method to determine the existence of REV. It can be gleaned from the study that the existence condition of fractured rock mass with medium tractility features average fracture spacing smaller than 0.6 m. If average fracture spacing is larger than 0.6 m, there is no existence of REV. The rationality of the model is verified by a case study. The present research provides a method for the simulation of seepage field in fissured rocks.

  20. Identification of Small Molecule Translesion Synthesis Inhibitors That Target the Rev1-CT/RIR Protein-Protein Interaction.

    Science.gov (United States)

    Sail, Vibhavari; Rizzo, Alessandro A; Chatterjee, Nimrat; Dash, Radha C; Ozen, Zuleyha; Walker, Graham C; Korzhnev, Dmitry M; Hadden, M Kyle

    2017-07-21

    Translesion synthesis (TLS) is an important mechanism through which proliferating cells tolerate DNA damage during replication. The mutagenic Rev1/Polζ-dependent branch of TLS helps cancer cells survive first-line genotoxic chemotherapy and introduces mutations that can contribute to the acquired resistance so often observed with standard anticancer regimens. As such, inhibition of Rev1/Polζ-dependent TLS has recently emerged as a strategy to enhance the efficacy of first-line chemotherapy and reduce the acquisition of chemoresistance by decreasing tumor mutation rate. The TLS DNA polymerase Rev1 serves as an integral scaffolding protein that mediates the assembly of the active multiprotein TLS complexes. Protein-protein interactions (PPIs) between the C-terminal domain of Rev1 (Rev1-CT) and the Rev1-interacting region (RIR) of other TLS DNA polymerases play an essential role in regulating TLS activity. To probe whether disrupting the Rev1-CT/RIR PPI is a valid approach for developing a new class of targeted anticancer agents, we designed a fluorescence polarization-based assay that was utilized in a pilot screen for small molecule inhibitors of this PPI. Two small molecule scaffolds that disrupt this interaction were identified, and secondary validation assays confirmed that compound 5 binds to Rev1-CT at the RIR interface. Finally, survival and mutagenesis assays in mouse embryonic fibroblasts and human fibrosarcoma HT1080 cells treated with cisplatin and ultraviolet light indicate that these compounds inhibit mutagenic Rev1/Polζ-dependent TLS in cells, validating the Rev1-CT/RIR PPI for future anticancer drug discovery and identifying the first small molecule inhibitors of TLS that target Rev1-CT.

  1. Molecular typing of Brucella melitensis endemic strains and differentiation from the vaccine strain Rev-1.

    Science.gov (United States)

    Noutsios, Georgios T; Papi, Rigini M; Ekateriniadou, Loukia V; Minas, Anastasios; Kyriakidis, Dimitrios A

    2012-03-01

    In the present study forty-four Greek endemic strains of Br. melitensis and three reference strains were genotyped by Multi locus Variable Number Tandem Repeat (ML-VNTR) analysis based on an eight-base pair tandem repeat sequence that was revealed in eight loci of Br. melitensis genome. The forty-four strains were discriminated from the vaccine strain Rev-1 by Restriction Fragment Length Polymorphism (RFLP) and Denaturant Gradient Gel Electrophoresis (DGGE). The ML-VNTR analysis revealed that endemic, reference and vaccine strains are genetically closely related, while most of the loci tested (1, 2, 4, 5 and 7) are highly polymorphic with Hunter-Gaston Genetic Diversity Index (HGDI) values in the range of 0.939 to 0.775. Analysis of ML-VNTRs loci stability through in vitro passages proved that loci 1 and 5 are non stable. Therefore, vaccine strain can be discriminated from endemic strains by allele's clusters of loci 2, 4, 6 and 7. RFLP and DGGE were also employed to analyse omp2 gene and reveled different patterns among Rev-1 and endemic strains. In RFLP, Rev-1 revealed three fragments (282, 238 and 44 bp), while endemic strains two fragments (238 and 44 bp). As for DGGE, the electrophoretic mobility of Rev-1 is different from the endemic strains due to heterologous binding of DNA chains of omp2a and omp2b gene. Overall, our data show clearly that it is feasible to genotype endemic strains of Br. melitensis and differentiate them from vaccine strain Rev-1 with ML-VNTR, RFLP and DGGE techniques. These tools can be used for conventional investigations in brucellosis outbreaks.

  2. Hard bottom substrate monitoring Horns Rev offshore wind farm. Annual status report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S.B.; Pedersen, John

    2004-05-15

    Elsam and Eltra built the offshore demonstration wind farm at Horns Rev in the North Sea. Elsam is the owner and is responsible for the operation of the wind farm. Eltra is responsible for the connection of the wind farm to the national onshore grid. In the summer months of 2002, Elsam constructed the world's largest offshore wind farm off the Danish west coast. The wind farm is sited 14-20 km into the North Sea, west of Blaevands Huk. The first wind turbine was erected in May 2002 and the last wind turbine tower of a total of 80 was in place by August 2002. The construction work was completed with the last connecting cables sluiced down in September 2002. All the wind turbines were in production by December 2002. The expected impact of the wind farm will primarily be an alternation of habitats due to the introduction of hard bottom substrates as wind turbine towers and scour protections. A continuous development in the epifouling communities will be expected together with an introduction of new or alien species in the area. The indigenous benthic community in the area of Horns Rev can be characterised by infauna species belonging to the Goniadella-Spisula community. This community is typical of sandbanks in the North Sea area, although communities in such areas are very variable and site-specific. Character species used as indicators for environmental changes in the Horns Rev area are the bristle worms Goniadella bobretzkii, Ophelia borealis, Psione remota and Orbinia sertulata and the mussels Goodallia triangularis and Spisula solida. In connection with the implementation of the monitoring programme concerning the ecological impact of the introduction of hard substrate related to the Horns Rev Wind Farm, surveys on hard bottom substrate was conducted in March 2003 and in September 2003. This report describes the first year results of surveys on hard substrate after the completion of the offshore wind farm at Horns Rev. (au)

  3. Offshore wind farms in the local community - a survey at Horns Rev wind farm. Background report; Havvindmoeller i lokalomraedet - en undersoegelse ved Horns Rev havmoellepark. Baggrundsrapport

    Energy Technology Data Exchange (ETDEWEB)

    Kuehn, S.

    2005-07-15

    This report is part of a socio-economic project about offshore wind farms' influence on the local community. The project is part of the monitoring programme in connection with the establishment of the offshore wind farms at Horns Rev and Nysted. The socio-economic project consists of a sociological and an environment-economic project. This report is part of the sociological project which has the purpose of identifying attitudes towards the wind farm before and after the erection with a view to identify possible changes in opinions and reasons for these changes. (BA)

  4. Non-preference of the red mite Oligonychus yothersi McGregor (Acari: Tetranychidae for oviposition on leaves of Paraguay tea progenies (Ilex paraguariensis/ Não-preferência do ácaro-vermelho, Oligonychus yothersi McGregor (Acari: Tetranychidae, para oviposição em folhas de progênies de erva-mate (Ilex paraguariensis

    Directory of Open Access Journals (Sweden)

    José Djair Vendramim

    2007-10-01

    Full Text Available The oviposition preference of the red mite Oligonychus yothersi McGregor was evaluated under laboratory in 52 Paraguay tea progenies and one control collected in a local farm. The experiments were conducted on Paraguay tea leaf discs infested with one adult female mite/disc, incubated in a climate chamber (25±1ºC, 14h photophase for five days. Evaluations were performed daily, by counting the number of eggs laid in the period, the number of female escapes in the first 48 hours, as well as the number of eggs laid before the escaping. It was verified that discrimination by the mite occurred on two groups of progenies, in relation to the number of eggs: one group was different from the control (53.8% of the progenies and the other was identical to it (46.2% of the progenies. In the first group, 60.7% of progenies were less oviposited while a higher number of eggs/female than the control was verified in 39.3% of them. In 57.7% of progenies there was escape of some mites (10 to 40%, and in 10 of these progenies no eggs were laid. The data provide evidences that in some of the progenies evaluated there is resistance by non-preference to oviposition of the Paraguay tea red mite.A preferência para oviposição do ácaro-vermelho, Oligonychus yothersi McGregor, foi avaliada em condições de laboratório em 52 progênies de erva-mate e uma testemunha coletada em uma propriedade da região. Os experimentos foram realizados em discos foliares de erva-mate (2 cm de diâmetro, infestados com uma fêmea adulta do ácaro, e incubados a 25±1ºC e 14h de fotofase, durante cinco dias. As avaliações foram realizadas diariamente, contando-se o número de ovos depositados no período, número de fêmeas que fugiram nas primeiras 48 horas e número de ovos depositados antes da fuga. Verificou-se que houve discriminação do ácaro em dois grupos de progênies, em relação ao número de ovos, um que diferiu em relação à testemunha (53,8% de progênies e

  5. Caracterização morfológica, determinação do nível de ploidia e viabilidade do pólen de uma progênie de tangerineira 'Clementina Fina' e 'Montenegrina'

    Directory of Open Access Journals (Sweden)

    Roberto Luis Weiler

    2011-01-01

    Full Text Available As plantas cítricas possuem ampla variação morfológica e citogenética. Neste contexto, análises dessas plantas podem auxiliar na identificação de materiais mais promissores para cruzamentos. Uma população de tangerineiras híbridas oriundas do cruzamento das tangerineiras 'Clementina Fina' (Citrus clementina Hort. ex Tan. e 'Montenegrina' (Citrus deliciosa Ten. foi avaliada segundo características morfológicas, época de maturação, número cromossômico e viabilidade de pólen. Foi possível distinguir as 94 plantas da progênie e os genitores através dos dados morfológicos. Verificou-se uma alteração no período de maturação dos frutos nas plantas híbridas. Todas as plantas avaliadas são diplóides com um número cromossômico de 2n=18, bem como, alto grau de viabilidade de pólen, variando entre 79,0% e 98,1%.

  6. Development and application of graphite-SiO2/Al2O3/Nb2O5-methylene blue (GRP-SiAlNb-MB composite for electrochemical determination of dopamine

    Directory of Open Access Journals (Sweden)

    Juliana de Fátima Giarola

    2017-03-01

    Full Text Available In the present paper an amperometric sensor based on graphite-SiO2/Al2O3/Nb2O5-methylene blue (GRP-SiAlNb-MB composite has been successfully prepared for dopamine (DA determination in real samples. The electrochemical behavior of DA at the GRP-SiAlNb-MB has been evaluated by employing cyclic voltammetry. The best ratio (m/m of GRP-SiAlNb-MB composite was found to be 1:0.54. Under optimized conditions (pH 7.5 in 0.15 mol L−1 phosphate buffer the amperometry method responds linearly to DA from 5.0 up to 500.0 μmol L−1 (r = 0.995 with limits of detection and quantification of 1.49 and 4.97 μmol L−1, respectively. The developed method was successfully applied for DA determination in real samples of pharmaceutical formulations and can be used for routine quality control analysis of pharmaceutical formulations containing DA. The use of inorganic matrix SiAlNb was found to be very useful to adsorb MB in the composite material with further improvement of the anodic peak current of DA.

  7. MicroRNA-340 inhibits the proliferation and promotes the apoptosis of colon cancer cells by modulating REV3L

    Science.gov (United States)

    Arivazhagan, Roshini; Lee, Jaesuk; Bayarsaikhan, Delger; Kwak, Peter; Son, Myeongjoo; Byun, Kyunghee; Salekdeh, Ghasem Hosseini; Lee, Bonghee

    2018-01-01

    DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) has recently emerged as an important oncogene. Although the expressions of REV3L are similar in normal and cancer cells, several mutations in REV3L have been shown to play important roles in cancer. These mutations cause proteins misfolding and mislocalization, which in turn alters their interactions and biological functions. miRNAs play important regulatory roles during the progression and metastasis of several human cancers. This study was undertaken to determine how changes in the location and interactions of REV3L regulate colon cancer progression. REV3L protein mislocalization confirmed from the immunostaining results and the known interactions of REV3L was found to be broken as seen from the PLA assay results. The mislocalized REV3L might interact with new proteins partners in the cytoplasm which in turn may play role in regulating colon cancer progression. hsa-miR-340 (miR-340), a microRNA down-regulated in colon cancer, was used to bind to and downregulate REV3L, and found to control the proliferation and induce the apoptosis of colon cancer cells (HCT-116 and DLD-1) via the MAPK pathway. Furthermore, this down-regulation of REV3L also diminished colon cancer cell migration, and down-regulated MMP-2 and MMP-9. Combined treatment of colon cancer cells with miR-340 and 5-FU enhanced the inhibitory effects of 5-FU. In addition, in vivo experiments conducted on nude mice revealed tumor sizes were smaller in a HCT-116-miR-340 injected group than in a HCT-116-pCMV injected group. Our findings suggest mutations in REV3L causes protein mislocalization to the cytoplasm, breaking its interaction and is believed to form new protein interactions in cytoplasm contributing to colon cancer progression. Accordingly, microRNA-340 appears to be a good candidate for colon cancer therapy. PMID:29435169

  8. Identification of Brucella melitensis Rev.1 vaccine-strain genetic markers: Towards understanding the molecular mechanism behind virulence attenuation.

    Science.gov (United States)

    Issa, Mohammad Nouh; Ashhab, Yaqoub

    2016-09-22

    Brucella melitensis Rev.1 is an avirulent strain that is widely used as a live vaccine to control brucellosis in small ruminants. Although an assembled draft version of Rev.1 genome has been available since 2009, this genome has not been investigated to characterize this important vaccine. In the present work, we used the draft genome of Rev.1 to perform a thorough genomic comparison and sequence analysis to identify and characterize the panel of its unique genetic markers. The draft genome of Rev.1 was compared with genome sequences of 36 different Brucella melitensis strains from the Brucella project of the Broad Institute of MIT and Harvard. The comparative analyses revealed 32 genetic alterations (30 SNPs, 1 single-bp insertion and 1 single-bp deletion) that are exclusively present in the Rev.1 genome. In silico analyses showed that 9 out of the 17 non-synonymous mutations are deleterious. Three ABC transporters are among the disrupted genes that can be linked to virulence attenuation. Out of the 32 mutations, 11 Rev.1 specific markers were selected to test their potential to discriminate Rev.1 using a bi-directional allele-specific PCR assay. Six markers were able to distinguish between Rev.1 and a set of control strains. We succeeded in identifying a panel of 32 genome-specific markers of the B. melitensis Rev.1 vaccine strain. Extensive in silico analysis showed that a considerable number of these mutations could severely affect the function of the associated genes. In addition, some of the discovered markers were able to discriminate Rev.1 strain from a group of control strains using practical PCR tests that can be applied in resource-limited settings. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Anti-pancreatic cancer activity of ONC212 involves the unfolded protein response (UPR) and is reduced by IGF1-R and GRP78/BIP.

    Science.gov (United States)

    Lev, Avital; Lulla, Amriti R; Wagner, Jessica; Ralff, Marie D; Kiehl, Joshua B; Zhou, Yan; Benes, Cyril H; Prabhu, Varun V; Oster, Wolfgang; Astsaturov, Igor; Dicker, David T; El-Deiry, Wafik S

    2017-10-10

    Pancreatic cancer is chemo-resistant and metastasizes early with an overall five-year survival of ∼8.2%. First-in-class imipridone ONC201 is a small molecule in clinical trials with anti-cancer activity. ONC212, a fluorinated-ONC201 analogue, shows preclinical efficacy in melanoma and hepatocellular-cancer models. We investigated efficacy of ONC201 and ONC212 against pancreatic cancer cell lines ( N =16 including 9 PDX-cell lines). We demonstrate ONC212 efficacy in 4 in-vivo models including ONC201-resistant tumors. ONC212 is active in pancreatic cancer as single agent or in combination with 5-fluorouracil, irinotecan, oxaliplatin or RTK inhibitor crizotinib. Based on upregulation of pro-survival IGF1-R in some tumors, we found an active combination of ONC212 with inhibitor AG1024, including in vivo . We show a rationale for targeting pancreatic cancer using ONC212 combined with targeting the unfolded-protein response and ER chaperones such as GRP78/BIP. Our results lay the foundation to test imipridones, anti-cancer agents, in pancreatic cancer, that is refractory to most drugs.

  10. Administrative Cicular No. 31 (Rev. 2) - International indemnity and non-resident allowance

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 31 (Rev. 2) entitled "International indemnity and non-resident allowance", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 23 June 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208547.   This revised circular cancels and replaces Administrative Circular No. 31 (Rev. 1) also entitled "International indemnity and non-resident allowance" of October 2007. The main changes reflect the decision taken in the framework of the five-yearly review to extend eligibility for international indemnity to all staff members, as well to introduce a distinction between current staff members and those recruited as from 1st September 2016. For the latter, the international indemnity will be calculated as a percentage of the minimum salary of the grade into which they are recruited; the amount granted to the former will not change, and is now expressed ...

  11. Administrative Circular No. 11 (Rev. 3) - Categories of members of the personnel

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 11 (Rev. 3) entitled “Categories of members of the personnel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 3 July 2014 and entering into force on 1 September 2014, is available on the intranet site of the Human Resources Department.   This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 11 (Rev. 2) entitled “Categories of members of the personnel” of January 2013. The circular was revised in order to include a minor adjustment of the determination of required period of break in the payment of subsistence allowance to certain categories of associated members of the personnel (taking account of possible technical means of control). Furthermore, the possibility of traineeships of long duration was restricted to cases in which the traineeship is awarded pursuant to an agreement between CERN and a...

  12. Administrative Circular No. 13 (Rev. 3) - Guarantees for representatives of the personnel

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 13 (Rev. 3) entitled “Guarantees for representatives of the personnel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 5 December 2013 and entering into force on 1 January 2014, is available on the intranet site of the Human Resources Department (see here).   This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 13 (Rev. 2) entitled “Guarantees for members of the personnel holding representative office” of November 1992. The circular was revised in order to adapt the time given to the representatives of the personnel to perform their elective mandate and to ensure more transparency in their activities, by indicating, inter alia, the percentage of time worked in the framework of their mandate, as well as the training, activities and ensuing results. Department Head Office HR Department

  13. Administrative circular n°23 (Rev. 3) – Special working hours

    CERN Multimedia

    2013-01-01

    Administrative Circular No. 23 (Rev. 3) entitled “Special working hours”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 11 October 2012 and entering into force on 1 January 2013, is available on the intranet site of the Human Resources Department.   This circular is applicable to staff members and fellows. It cancels and replaces Administrative Circular No. 23 (Rev. 2) entitled “Special working hours” of December 2008. Paragraph 6 a) of Annex II of this circular was revised following the modification of Article III 1.04 of the Staff Regulations approved by Council on 14 December 2012. The modification serves to adapt the minimum rest time to the fact that, in case of rapidly alternating shifts, a maximum of seven consecutive shifts may be performed. Department Head Office HR Department

  14. Administrative circular n°11 (REV. 2) – Categories of members of the personnel

    CERN Multimedia

    2012-01-01

    Administrative Circular No. 11 (Rev. 2) entitled “Categories of members of the personnel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 11 May 2012 and entering into force on 1 January 2013, is available on the intranet site of the Human Resources Department. This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 11 (Rev. 1) entitled “Categories of members of the personnel” of January 1997 as regards all contracts of members of the personnel issued on or after 1 January 2013. The circular was revised in order to take into account developments since the last revision of the categories of personnel in 1997 as well as the needs of the Organization and collaborating institutes. In particular, it introduces a new system for distinguishing categories of associated members of the personnel, namely with regard to the purpo...

  15. Administrative circular No. 20 (Rev. 2) – Use of private vehicules for journeys on official duty

    CERN Multimedia

    HR Department

    2011-01-01

    Administrative Circular No. 20 (Rev. 2) entitled "Use of private vehicles for journeys on official duty", adopted following discussion in the Standing Concertation Committee meeting of 21 September 2010 and entering into force on 1 January 2011, is available on the intranet site of the Human Resources Department: http://cern.ch/hr-docs/admincirc/admincirc.asp It cancels and replaces Administrative Circular No. 20 (Rev. 1) entitled "Use of private vehicles on official duty" of April 1993. This new version introduces, in particular, the payment of a kilometer allowance in case of emergency during standby duty or when on call and a simplified calculation of the allowance for journeys between sites. This circular also clarifies the type of permitted private vehicles. Department Head Office  

  16. Rev and Rex proteins of human complex retroviruses function with the MMTV Rem-responsive element

    Directory of Open Access Journals (Sweden)

    Dudley Jaquelin P

    2009-02-01

    Full Text Available Abstract Background Mouse mammary tumor virus (MMTV encodes the Rem protein, an HIV Rev-like protein that enhances nuclear export of unspliced viral RNA in rodent cells. We have shown that Rem is expressed from a doubly spliced RNA, typical of complex retroviruses. Several recent reports indicate that MMTV can infect human cells, suggesting that MMTV might interact with human retroviruses, such as human immunodeficiency virus (HIV, human T-cell leukemia virus (HTLV, and human endogenous retrovirus type K (HERV-K. In this report, we test whether the export/regulatory proteins of human complex retroviruses will increase expression from vectors containing the Rem-responsive element (RmRE. Results MMTV Rem, HIV Rev, and HTLV Rex proteins, but not HERV-K Rec, enhanced expression from an MMTV-based reporter plasmid in human T cells, and this activity was dependent on the RmRE. No RmRE-dependent reporter gene expression was detectable using Rev, Rex, or Rec in HC11 mouse mammary cells. Cell fractionation and RNA quantitation experiments suggested that the regulatory proteins did not affect RNA stability or nuclear export in the MMTV reporter system. Rem had no demonstrable activity on export elements from HIV, HTLV, or HERV-K. Similar to the Rem-specific activity in rodent cells, the RmRE-dependent functions of Rem, Rev, or Rex in human cells were inhibited by a dominant-negative truncated nucleoporin that acts in the Crm1 pathway of RNA and protein export. Conclusion These data argue that many retroviral regulatory proteins recognize similar complex RNA structures, which may depend on the presence of cell-type specific proteins. Retroviral protein activity on the RmRE appears to affect a post-export function of the reporter RNA. Our results provide additional evidence that MMTV is a complex retrovirus with the potential for viral interactions in human cells.

  17. Effects on birds of an offshore wind park at Horns Rev: Environmental impact assessment

    Energy Technology Data Exchange (ETDEWEB)

    Noer, H.; Kjaer Christensen, T.; Clausager, I.; Krag Petersen, I. [DMU, Dept. of Coastal Zone Ecology (Denmark)

    2000-07-01

    This report presents the technical background to the ornithological environmental impact assessment for the construction of an offshore windpark at Horns Rev, 14 km west-south-west of Blaevandshuk, Denmark. Construction of the park is planned to commence in 2001. The park will consist of c. 80 wind turbines, each of at least 1.8 MW, and cover an area of 27.5 km{sup 2} (including the 200 m exclusion zone around the park). (au)

  18. Determination of Geometrical REVs Based on Volumetric Fracture Intensity and Statistical Tests

    Directory of Open Access Journals (Sweden)

    Ying Liu

    2018-05-01

    Full Text Available This paper presents a method to estimate a representative element volume (REV of a fractured rock mass based on the volumetric fracture intensity P32 and statistical tests. A 150 m × 80 m × 50 m 3D fracture network model was generated based on field data collected at the Maji dam site by using the rectangular window sampling method. The volumetric fracture intensity P32 of each cube was calculated by varying the cube location in the generated 3D fracture network model and varying the cube side length from 1 to 20 m, and the distribution of the P32 values was described. The size effect and spatial effect of the fractured rock mass were studied; the P32 values from the same cube sizes and different locations were significantly different, and the fluctuation in P32 values clearly decreases as the cube side length increases. In this paper, a new method that comprehensively considers the anisotropy of rock masses, simplicity of calculation and differences between different methods was proposed to estimate the geometrical REV size. The geometrical REV size of the fractured rock mass was determined based on the volumetric fracture intensity P32 and two statistical test methods, namely, the likelihood ratio test and the Wald–Wolfowitz runs test. The results of the two statistical tests were substantially different; critical cube sizes of 13 m and 12 m were estimated by the Wald–Wolfowitz runs test and the likelihood ratio test, respectively. Because the different test methods emphasize different considerations and impact factors, considering a result that these two tests accept, the larger cube size, 13 m, was selected as the geometrical REV size of the fractured rock mass at the Maji dam site in China.

  19. Hard bottom substrate monitoring Horns Rev offshore wind farm. Annual status report. 2004

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S.B.; Pedersen, John

    2005-05-15

    Elsam and Eltra have built the offshore demonstration wind farm at Horns Rev in the North Sea. Elsam is the owner and is responsible for the operation of the wind farm. Eltra is responsible for the connection of the wind farm to the national onshore grid. In the summer months of 2002, Elsam constructed the world's largest offshore wind farm at the Danish west coast. The wind farm is located 14-20 km into the North Sea, west of Blaevands Huk. The first wind turbine foundation was in place in March 2002 and the last mono-pile was in place in August 2002 for a total of 80. The construction work was completed with the last connecting cables sluiced down in September 2002. All the wind turbines were in production in December 2002. The expected impact from the wind farm will primarily be an alternation of habitats due to the introduction of hard bottom substrates as wind mono-piles and scour protections. A continuous development in the epifouling communities will be expected together with an introduction of new or alien species in the area. The indigenous benthic community in the area of Horn Rev can be characterised by infauna species belonging to the Goniadella-Spisula community. This community is typical of sandbanks in the North Sea area, although communities in such areas are very variable and site specific. Character species used as indicators for environmental changes in the Horns Rev area are the bristle worms Goniadella bobretzkii, Ophelia borealis, Psione remota and Orbinia sertulata and the mussels Goodallia triangularis and Spisula solida. In connection with the implementation of the monitoring programme concerning the ecological impact of the introduction of hard substrate related to the Horns Rev Wind Farm, surveys on hard bottom substrates were initialised in March 2003 with monitoring conducted in September 2003 and March and September 2004. This report describes the results from surveys on hard substrates in 2004. (au)

  20. Hard bottom substrate monitoring Horns Rev offshore wind farm. Annual status report. 2004

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S B; Pedersen, John

    2005-05-15

    Elsam and Eltra have built the offshore demonstration wind farm at Horns Rev in the North Sea. Elsam is the owner and is responsible for the operation of the wind farm. Eltra is responsible for the connection of the wind farm to the national onshore grid. In the summer months of 2002, Elsam constructed the world's largest offshore wind farm at the Danish west coast. The wind farm is located 14-20 km into the North Sea, west of Blaevands Huk. The first wind turbine foundation was in place in March 2002 and the last mono-pile was in place in August 2002 for a total of 80. The construction work was completed with the last connecting cables sluiced down in September 2002. All the wind turbines were in production in December 2002. The expected impact from the wind farm will primarily be an alternation of habitats due to the introduction of hard bottom substrates as wind mono-piles and scour protections. A continuous development in the epifouling communities will be expected together with an introduction of new or alien species in the area. The indigenous benthic community in the area of Horn Rev can be characterised by infauna species belonging to the Goniadella-Spisula community. This community is typical of sandbanks in the North Sea area, although communities in such areas are very variable and site specific. Character species used as indicators for environmental changes in the Horns Rev area are the bristle worms Goniadella bobretzkii, Ophelia borealis, Psione remota and Orbinia sertulata and the mussels Goodallia triangularis and Spisula solida. In connection with the implementation of the monitoring programme concerning the ecological impact of the introduction of hard substrate related to the Horns Rev Wind Farm, surveys on hard bottom substrates were initialised in March 2003 with monitoring conducted in September 2003 and March and September 2004. This report describes the results from surveys on hard substrates in 2004. (au)

  1. Losers, Food, and Sex. Clerical Masculinity in the BBC Sitcom Rev.

    Directory of Open Access Journals (Sweden)

    Alexander Ornella

    2016-11-01

    Full Text Available Clerical masculinities, much like their lay/secular counterparts, often appear unchang¬ing because they are the products of naturalization processes. Clerical masculinities, however, are far from stable, for they live and breathe the dynamics of both their socio-religious context and their secular “others”. The BBC sitcom Rev. (BBC2, UK 2010–2014 is a refreshing take on the everyday life and problems of a vicar in the Church of England trying to avoid stereotypes that often come with clerical roles. Rev. (2010–2014 can be interpreted as an attempt to explore the negotiation processes of masculinity within an institution that is involved in the “production” of religion and gender roles. It shows that being a man in an institutional setting is as much a perfor¬mance as it is a more or less successful negotiation of other people’s expectations and one’s own worldview. In particular, the main male clerical characters in Rev. (2010– 2014 inhabit a position of power but all have their flaws. They can best be understood as losers whose clash with masculine systems renders them more human.

  2. Guidance and considerations for implementation of INFCIRC/225/Rev.3, the physical protection of nuclear material

    International Nuclear Information System (INIS)

    1997-09-01

    The Physical Protection of Nuclear Material, INFCIRC/225/Rev.3, provides recommendations for the physical protection of nuclear material against theft in use, storage and transport, whether national or international and whether peaceful or military, and contains provisions relating to the sabotage of nuclear material or facilities. The recommendations contained in INFCIRC/225/Rev.3 detail the elements that should be included in a State's system of physical protection. It also recognizes the adverse health and safety consequences arising from the theft of nuclear material and the sabotage of nuclear material or facilities. Most industrial and developing countries use these recommendations to some extent in the establishment and operation of their physical protection systems. Although INFCIRC/225/Rev.3 provides recommendations for protecting materials and facilities from theft or sabotage, it does not provide in-depth details for these recommendations. In June 1996, the IAEA convened a consultants meeting to consider this matter. This report is the result of continuing discussions and drafts over a period of nine months. The intent of this guidance is to provide a broader basis for relevant State organizations to prescribe appropriate requirements for the use of nuclear materials which are compatible with accepted international practice

  3. Melhoramento do cafeeiro: XXXVIII. Observações sobre progênies do cultivar Mundo-Novo de Coffea arabica na estação experimental de Mococa Coffee breeding: XXXVIII-observation on progenies of the Mundo-Novo cultivars of Coffea arabica in the Mococa experimental station

    Directory of Open Access Journals (Sweden)

    Túlio R. Rocha

    1980-01-01

    Full Text Available Os dados analisados no experimento localizado em Mococa sobre a produtividade de 112 progênies dos cultivares Mundo-Novo S1 e S2, Bourbon-Amarelo, BourbonVermelho e Caturra-Vermelho de Coffea arabica no período de 1955 a 1971, indicaram que as de Mundo-Novo S1, de prefixos MP 474, MP 502, MP 469, MP 492 e MP 475, revelaram-se como as mais produtivas, assemelhando-se a algumas progênies 'Mundo--Novo' S2. Dentre estas, destacou-se a de prefixo MP 388-6, que atingiu o nível mais elevado de produção do experimento. As progênies de 'Mundo-Novo', em conjunto, produziram 44% a mais do que as de Bourbon-Amarelo e, estas, 60% a mais do que as de Bourbon-Vermelho e Caturra-Vermelho. A altura e o diâmetro da copa atingiram valores médios mais elevados para as progênies de 'Mundo-Novo'. Verificaram-se correlações positivas e altamente significativas entre altura média da planta e diâmetro médio da copa com a produção das progênies. As progênies mais produtivas revelaram rendimento (relação entre peso de café maduro e beneficiado de aproximadamente 6,0 e porcentagem de sementes normais, do tipo chato, acima de 80. Quanto ao tamanho das sementes do tipo chato, duas progênies 'Mundo-Novo' S1, MP 474 e MP 452, apresentaram peneira média maior, permi-tindo seleção de plantas com essa característica e com elevada produção.Coffee progenies of the Mundo-Novo cultivars of Coffea arabica were studied in an experiment located at the Mococa Experimental Station of the Instituto Agronômico in comparison with Bourbon-Amarelo, Bourbon-Vermelho and Caturra-Vermelho cultivars of the same species. During a period of 17 consecutive cropping years (1955-1971, Mundo-Novo yielded approximately 44% more than Bourbon-Amarelo and this cultivars yielded 60% more than Bourbon-Vermelho and Caturra-Vermelho. Among the 89 S1 'Mundo-Novo' progenies, MP 474, MP 502, MP 469, MP 492 and MP 475 yielded as much as the two best 'Mundo-Novo' S2 progenies. Greater

  4. Variação genética em progênies de Myracrodruon urundeuva F.F. & M.F. Allemão em três sistemas de cultivo Genetic variation in progenies of Myracrodruon urundeuva F.F. & M.F. Allemao in three cultivation systems

    Directory of Open Access Journals (Sweden)

    Miguel Luiz Menezes Freitas

    2006-06-01

    Full Text Available Myracrodruon urundeuva (aroeira é uma das espécies arbóreas que apresentam madeira de maior durabilidade em contato com o solo. Em razão dessa característica, populações naturais de M. urundeuva, distribuídas por quase todo o Brasil, vêm sendo dizimadas. Desse modo, o presente trabalho teve por objetivo avaliar a expressão da variação genética em uma população natural de M. urundeuva procedente da Estação Ecológica do Instituto Florestal, localizada em Paulo de Faria, SP. Coletaram-se sementes de 30 árvores de polinização livre, em setembro de 1996. Com esse material, foram instalados três testes de progênies em diferentes sistemas de plantio em Selvíria, MS, obedecendo a um delineamento em blocos casualizados, com 30 tratamentos (progênies e três repetições. Foi assumido o modelo de cruzamento misto para essa espécie. Verificou-se que houve diferença significativa nos efeitos de progênies e ambiente (testes de progênies, e a interação progênies x ambientes não foi significativa. Desse modo, as progênies apresentaram comportamento semelhante nos diferentes ambientes. A melhor "performance" das progênies foi no plantio envolvendo M. urundeuva, Guazuma ulmifolia (Lam e Anandenanthera falcata (Benth. Speg., as quais também apresentaram considerável variação genética, indicando alto valor para programas de conservação e melhoramento genético.Myracrodruon urundeuva is one of the tree species the wood that presents the longest durability when in contact with soil. Because of this characteristic, populations of M. urundeuva have been decimated throughout Brazil. Therefore, this work aimed at analyzing the expression of genetic variation in a natural population of M. urundeuva from the Ecological Station of the Forest Institute, located in Paulo de Faria-SP. Seeds were collected from 30 free-pollination trees in September, 1996. By using this material, three progeny trials were installed in different

  5. Progênies de Segunda Geração de Grevilea: Produção de Madeira na Região de Londrina, PR Second Generation of Grevillea: Wood Production in Londrina-PR Region

    Directory of Open Access Journals (Sweden)

    Emerson Gonçalves Martins

    2011-03-01

    Full Text Available

    O uso da grevílea (Grevillea robusta Cunn não se restringe somente ao sombreamento de cafezais, ornamentação e quebra-ventos, é também usada pela apicultura e na produção de madeira para usos diversos. O presente trabalho objetivou estimar a produção de madeira e estabelecer fontes de sementes melhoradas para plantios comerciais na região de Londrina, PR. Para tal, um experimento com 37 progênies de segunda geração foi instalado no Distrito de Maravilha, Município de Londrina, PR, com coordenadas geográficas 23°23’30"S e 51°11’05"W. O delineamento experimental utilizado foi de blocos ao acaso com parcelas lineares de cinco plantas distribuídas aleatoriamente com oito repetições, num espaçamento de 3 m x 3 m. A partir das medições das alturas e dos diâmetros na altura do peito (DAP, a variável estimada (volume cilíndrico foi analisada para comparar as médias entre as progênies. Concluiu-se que em Londrina, a família mais produtiva em volume e com maior sobrevivência é da procedência australiana Duck Creek (NSW. 
    Grevillea robusta Cunn is not only utilized for coffee plantation shading, ornamentation and wind breaks. It is also used in apiculture and different wood utilities. The purpose of this study was to identify genetically superior individuals for wood volume production in order to select material for future seed orchards in the Londrina region of Northern Paraná State, Brazil. The genetic material tested was 37 second generation half-sib progenies from a provenance trial located on Presidente Castelo Branco county, also in the State of Paraná. The experiment was planted in EMBRAPA’s Maravilha farm with the following geographic coordinates: 23°23’30"S and 51°11’05"W. Progenies were evaluated in a experimental design in the form of eight replications and five plants per plot. Height, diameter and volume were statistically analysed in order

  6. Genetic selection Pinus greggii progeny to the establishment of seed orchards Seleção genética de progênies de Pinus greggii para formação de pomares de sementes

    Directory of Open Access Journals (Sweden)

    Ananda Virgínia de Aguiar

    2010-10-01

    Full Text Available The main deterrent to the adoption of new species for operational plantations is the availability of  genetically improved seeds in sufficient amount to supply the  market. Therefore, this study was carried out with the objective to estimate genetic parameters for individual selection as a basis to transform the field trial into a seedling seed orchard. The trial contained forty seven half-sib families from five Mexican provenances, including northern and central Mexico sources. These were planted at Ponta Grossa, Paraná State, Brazil, in a
    randomized complete block design with nine  replications of five-tree linear plots in a 3 m x 3 m spacing. Height, dbh (diameter at breast height and stem form were assessed in thirteen-year old tree. Variance components were estimated by using SELEGEN-REML/BLUP software. Other procedures included prediction of breeding values and deviance analyses. The significant genetic variation among provenances as well as among and within families in all traits confirmed their high value. The southern Mexico sources showed the highest productivity. The expected progress in  improvement through just one cycle of selection was estimated in 21.6 % in wood volume.

    doi: 10.4336/2010.pfb.30.62.107
    Um dos principais impedimentos para a adoção de novas espécies para as plantações comerciais é a

    escassez de sementes geneticamente melhoradas, em quantidade suficiente para abastecer o mercado. Portanto,

    o objetivo deste estudo foi estimar parâmetros genéticos que possibilitem a realização de seleção individual em

    um teste combinado de procedências e progênies de P. greggii como base para transformar a área num pomar

    de sementes por mudas. No presente trabalho, foram avaliadas cinco procedências e quarenta e sete progênies

    de P. greggii da região norte e central do México. O experimento foi instalado

  7. Status report of seabird surveys at Horns Rev, 2000-2001

    International Nuclear Information System (INIS)

    Kjaer Christensen, T.; Clausager, I.; Krag Petersen, I.

    2002-01-01

    The present report presents the results of three bird surveys conducted in the Horns Rev area during the second half of 2001. Due to poor weather conditions in December 2001, the last survey was, however, performed on 7 January 2002. The surveys are part of the base-line investigations of birds performed in relation to the proposed construction of an offshore wind farm at Horns Rev in the Danish part of the North Sea ca 14 km southwest of Blaevandshuk. The results of the surveys during August 2001 - January 2002 are presented together with the results obtained during the period August 2000 - April 2001, and are also compared to results obtained during the period August 1999 - April 2000. Based on the distribution of the most abundant bird species recorded during 16 aerial surveys performed during August 1999 - January 2002, there were no indications that the wind farm area was of any particular importance to the birds' exploitation of the Horns Rev area. Fish-eating species like divers, gannet, terns, auks and gulls generally showed scattered and variable distributions, mainly occurring in the areas north and south of Horns Rev, and with low numbers on the reef proper and within the planned wind farm area. The distribution of benthic foraging species, eider and common Scoter, showed that they mainly exploited the coastal parts of the area off Blaevandshuk and Skallingen, although common scoter was found in relatively high numbers on the southeast slopes of the Horns Rev and within the wind farm area in the April 2001 survey. Common scoters occurred in very high numbers in January 2002. This was probably related to increased immigration of birds from the inner Danish waters during a cold period in late December 2001. Preference analyses of bird exploitation of the Horns Rev area showed that if the birds completely avoid the wind farm area after erection of the wind turbines, this will affect less than 1% of the various species, except divers where 1.58% will be

  8. LEDGF/p75 Overexpression Attenuates Oxidative Stress-Induced Necrosis and Upregulates the Oxidoreductase ERP57/PDIA3/GRP58 in Prostate Cancer.

    Directory of Open Access Journals (Sweden)

    Anamika Basu

    Full Text Available Prostate cancer (PCa mortality is driven by highly aggressive tumors characterized by metastasis and resistance to therapy, and this aggressiveness is mediated by numerous factors, including activation of stress survival pathways in the pro-inflammatory tumor microenvironment. LEDGF/p75, also known as the DFS70 autoantigen, is a stress transcription co-activator implicated in cancer, HIV-AIDS, and autoimmunity. This protein is targeted by autoantibodies in certain subsets of patients with PCa and inflammatory conditions, as well as in some apparently healthy individuals. LEDGF/p75 is overexpressed in PCa and other cancers, and promotes resistance to chemotherapy-induced cell death via the transactivation of survival proteins. We report in this study that overexpression of LEDGF/p75 in PCa cells attenuates oxidative stress-induced necrosis but not staurosporine-induced apoptosis. This finding was consistent with the observation that while LEDGF/p75 was robustly cleaved in apoptotic cells into a p65 fragment that lacks stress survival activity, it remained relatively intact in necrotic cells. Overexpression of LEDGF/p75 in PCa cells led to the upregulation of transcript and protein levels of the thiol-oxidoreductase ERp57 (also known as GRP58 and PDIA3, whereas its depletion led to ERp57 transcript downregulation. Chromatin immunoprecipitation and transcription reporter assays showed LEDGF/p75 binding to and transactivating the ERp57 promoter, respectively. Immunohistochemical analysis revealed significantly elevated co-expression of these two proteins in clinical prostate tumor tissues. Our results suggest that LEDGF/p75 is not an inhibitor of apoptosis but rather an antagonist of oxidative stress-induced necrosis, and that its overexpression in PCa leads to ERp57 upregulation. These findings are of significance in clarifying the role of the LEDGF/p75 stress survival pathway in PCa.

  9. DC Brushless Motor Control Design and Preliminary Testing for Independent 4-Wheel Drive Rev-11 Robotic Platform

    Directory of Open Access Journals (Sweden)

    Roni Permana Saputra

    2012-03-01

    Full Text Available This paper discusses the design of control system for brushless DC motor using microcontroller ATMega 16 that will be applied to an independent 4-wheel drive Mobile Robot LIPI version 2 (REV-11. The control system consists of two parts which are brushless DC motor control module and supervisory control module that coordinates the desired command to the motor control module. To control the REV-11 platform, supervisory control transmit the reference data of speed and direction of motor to control the speed and direction of each actuator on the platform REV-11. From the test results it is concluded that the designed control system work properly to coordinate and control the speed and direction of motion of the actuator motor REV-11 platform. 

  10. Horns Rev offshore wind farm. Environmental impact assessment of sea bottom and marine biology

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S.B.

    2000-03-15

    An Environmental Impact Assessment (EIA) of a planned 150 MW offshore wind farm at Horns Rev has been carried out for the marine biology and sea bottom in the area, and includes vegetation and benthic fauna. The study forms part of a total EIA of the planned offshore wind farm. This EIA study has been drawn up in accordance with the guidelines laid down by the Ministry of Environment and Energy in the publication, 'Guidelines for preparation of EIAstudies for offshore wind farms. Horns Rev is situated off Blaevands Huk, which is Denmark's most westerly point. It is a shallow reef with water depths between 2 and 9 metres and is primarily composed of sand, gravel and pebbles. The area designated for the wind farm lies directly south of Horns Rev and is dominated by sand with a median particle size of 0.3 mm. Along the edges, towards areas of greater depth, the particle size increases. There are areas of fine sand in the deepest area, and in isolated pockets within the proposed wind farm site. The sediment is characterised by a very low (<1%) organic matter content. On the basis of the expected impact from the establishment of the wind farm, it is not deemed necessary to carry out special programmes during the construction phase for monitoring of the environmental-biological conditions. A monitoring and control programme is recommended during the production phase in order to follow the copper concentration in bivalves, or alternatively to initiate recovery or elimination of the copper-laden waste. A control programme is recommended during the production phase in order to follow the establishment and succession of the fouling community on the wind turbine foundations and scour-protecting revetments. (BA)

  11. Horns Rev offshore wind farm. Environmental impact assessment of sea bottom and marine biology

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S B

    2000-03-15

    An Environmental Impact Assessment (EIA) of a planned 150 MW offshore wind farm at Horns Rev has been carried out for the marine biology and sea bottom in the area, and includes vegetation and benthic fauna. The study forms part of a total EIA of the planned offshore wind farm. This EIA study has been drawn up in accordance with the guidelines laid down by the Ministry of Environment and Energy in the publication, 'Guidelines for preparation of EIAstudies for offshore wind farms. Horns Rev is situated off Blaevands Huk, which is Denmark's most westerly point. It is a shallow reef with water depths between 2 and 9 metres and is primarily composed of sand, gravel and pebbles. The area designated for the wind farm lies directly south of Horns Rev and is dominated by sand with a median particle size of 0.3 mm. Along the edges, towards areas of greater depth, the particle size increases. There are areas of fine sand in the deepest area, and in isolated pockets within the proposed wind farm site. The sediment is characterised by a very low (<1%) organic matter content. On the basis of the expected impact from the establishment of the wind farm, it is not deemed necessary to carry out special programmes during the construction phase for monitoring of the environmental-biological conditions. A monitoring and control programme is recommended during the production phase in order to follow the copper concentration in bivalves, or alternatively to initiate recovery or elimination of the copper-laden waste. A control programme is recommended during the production phase in order to follow the establishment and succession of the fouling community on the wind turbine foundations and scour-protecting revetments. (BA)

  12. Bird numbers and distributions in the Horns Rev offshore wind farm area. Annual status report 2004

    Energy Technology Data Exchange (ETDEWEB)

    Krag Petersen, I.

    2005-07-01

    This report presents data from four aerial surveys of birds in the Horns Rev wind farm area in 2004. Three surveys from the winter and spring of 2004 are thoroughly reported here. The fourth survey of 9 September 2004 is reported in general terms, but not included in presentations of distribution and effect analyses of the wind farm. Data from this survey will be thoroughly dealt with in a future report. Including the four surveys of 2004, a total of 29 surveys have been performed in that area since August 1999. (au)

  13. Pilot power based rate control in CDMA 2000 1x EV-DO Rev-A

    Institute of Scientific and Technical Information of China (English)

    LIU Xiao-feng; GU Jian; YANG Hong-wen

    2008-01-01

    This article proposes a new algorithm to improvethe rate control efficiency of enhanced reverse link mediumaccess control (RLMAC) in the code division multiple access(CDMA) lx EV-DO release A(Rev. A) system. The newalgorithm brings reverse access terminal (AT) pilot power tothe RLMAC rate control procedure and makes it easier for alow pilot power user to increase its data rate when the systemis slightly loaded and harder to decrease its date rate when thesystem is heavily loaded. Numerical results of system levelsimulations show that the new algorithm can bring highersystem throughput, lower AT transmission power, and lowersystem load.

  14. Simulaton of the Avedøreværket Unit 1 Cogeneration Plant with DNA

    DEFF Research Database (Denmark)

    Elmegaard, Brian; Houbak, Niels

    2003-01-01

    The simulator contest proposed for the ECOS 2003 conference has been solved using the DNA energy system simulator. The contest concerns the steam process of the Avedøreværket Unit 1 (AVV1) power plant. The plant is a 250 MWCHP plant with a maximum district heat production of 330 MJ/s. The plant has...... a net electric efficiency of 42% and a maximum energy utilization of 92%. In this paper it is demonstrated, that the DNA model of AVV1 can calculate the whole flow sheet balance at any load point, i.e., any possible combination of power production and district heat production. The paper also contains...

  15. A novel, mouse mammary tumor virus encoded protein with Rev-like properties

    International Nuclear Information System (INIS)

    Indik, Stanislav; Guenzburg, Walter H.; Salmons, Brian; Rouault, Francoise

    2005-01-01

    We have identified a novel, multiple spliced, subgenomic mRNA species in MMTV producing cells of different origin containing an open reading frame encoding a 39-kDa Rev-like protein, Rem (regulator of expression of MMTV). An EGFP-Rem fusion protein is shown to be predominantly in the nucleolus. Further leptomycin B inhibits the nuclear export of nonspliced MMTV transcripts, implicating Rem in nuclear export by the Crm1 pathway in MMTV. Rem is thus reminiscent of the Rec protein from the related endogenous human retrovirus, HERV-K

  16. Permeability computation on a REV with an immersed finite element method

    International Nuclear Information System (INIS)

    Laure, P.; Puaux, G.; Silva, L.; Vincent, M.

    2011-01-01

    An efficient method to compute permeability of fibrous media is presented. An immersed domain approach is used to represent the porous material at its microscopic scale and the flow motion is computed with a stabilized mixed finite element method. Therefore the Stokes equation is solved on the whole domain (including solid part) using a penalty method. The accuracy is controlled by refining the mesh around the solid-fluid interface defined by a level set function. Using homogenisation techniques, the permeability of a representative elementary volume (REV) is computed. The computed permeabilities of regular fibre packings are compared to classical analytical relations found in the bibliography.

  17. Fundamental study on REV based on crack tensor at the Mizunami Underground Research Laboratory

    International Nuclear Information System (INIS)

    Tanno, Takeo; Sato, Toshinori; Sanada, Hiroyuki; Hikima, Ryoichi; Kumasaka, Hiroo; Tada, Hiroyuki

    2013-01-01

    The crack tensor model which is a kind of equivalent continuum model has been studied in rock mechanical investigation in the MIU. The fractured rock mass is modeled as the elastic continuum model with this crack tensor. In this study, this crack tensor based on the geological observation in the MIU project was calculated, and Representative Elementary Volume (REV) in the ventilation shaft and -300 m access/research gallery was studied based on the relative error of this crack tensor. As a result, the convergence of the relative error was faster in the -300 m access/research gallery than in the ventilation shaft. (author)

  18. The GRP1 PH domain, like the AKT1 PH domain, possesses a sentry glutamate residue essential for specific targeting to plasma membrane PI(3,4,5)P(3).

    Science.gov (United States)

    Pilling, Carissa; Landgraf, Kyle E; Falke, Joseph J

    2011-11-15

    During the appearance of the signaling lipid PI(3,4,5)P(3), an important subset of pleckstrin homology (PH) domains target signaling proteins to the plasma membrane. To ensure proper pathway regulation, such PI(3,4,5)P(3)-specific PH domains must exclude the more prevalant, constitutive plasma membrane lipid PI(4,5)P(2) and bind the rare PI(3,4,5)P(3) target lipid with sufficiently high affinity. Our previous study of the E17K mutant of the protein kinase B (AKT1) PH domain, together with evidence from Carpten et al. [Carpten, J. D., et al. (2007) Nature 448, 439-444], revealed that the native AKT1 E17 residue serves as a sentry glutamate that excludes PI(4,5)P(2), thereby playing an essential role in specific PI(3,4,5)P(3) targeting [Landgraf, K. E., et al. (2008) Biochemistry 47, 12260-12269]. The sentry glutamate hypothesis proposes that an analogous sentry glutamate residue is a widespread feature of PI(3,4,5)P(3)-specific PH domains, and that charge reversal mutation at the sentry glutamate position will yield both increased PI(4,5)P(2) affinity and constitutive plasma membrane targeting. To test this hypothesis, we investigated the E345 residue, a putative sentry glutamate, of the general receptor for phosphoinositides 1 (GRP1) PH domain. The results show that incorporation of the E345K charge reversal mutation into the GRP1 PH domain enhances PI(4,5)P(2) affinity 8-fold and yields constitutive plasma membrane targeting in cells, reminiscent of the effects of the E17K mutation in the AKT1 PH domain. Hydrolysis of plasma membrane PI(4,5)P(2) releases the E345K GRP1 PH domain into the cytoplasm, and the efficiency of this release increases when Arf6 binding is disrupted. Overall, the findings provide strong support for the sentry glutamate hypothesis and suggest that the GRP1 E345K mutation will be linked to changes in cell physiology and human pathologies, as demonstrated for AKT1 E17K [Carpten, J. D., et al. (2007) Nature 448, 439-444; Lindhurst, M. J., et al

  19. Estimativas de parâmetros genéticos e ganho de seleção para produção de frutos em progênies de polinização aberta de pupunheira no estado do Pará, Brasil

    Directory of Open Access Journals (Sweden)

    João Tomé de Farias Neto

    2013-06-01

    Full Text Available O estudo objetivou estimar parâmetros e valores genéticos para os caracteres número de cachos (NC, peso médio do cacho (PMC e peso total do cacho (PTC aos seis anos, pelo método de modelos mistos (REML/BLUP. O teste de progênies foi estabelecido sob o delineamento de blocos ao acaso com 50 tratamentos (progênies, 10 blocos e uma planta por parcela, no espaçamento de 5 x 5 m, além de uma bordadura externa ao experimento. As estimativas de herdabilidade individual e médias no sentido restrito para PTC (0,213 e 0,360 e NC (0,286 e 0,435, respectivamente, foram de boas magnitudes. O coeficiente de variação genética individual exibiu valores elevados para PTC (19,86 e NC (17,59. A raiz quadrada da herdabilidade ao nível de médias das progênies resulta em acurácia seletiva na ordem de 0,60 para PTC e 0,66 para NC, atestando boa precisão e confiança nos parâmetros genéticos estimados, promovendo segurança na seleção. O potencial da população para fins de melhoramento pode ser observado por meio dos ganhos genéticos para PTC, que oscilaram de 8,3 a 19,9 kg, elevando a nova média populacional para o caráter após um ciclo de seleção, para 40,4 kg, equivalente a 25,8%.

  20. High amplitude phase resetting in rev-erbalpha/per1 double mutant mice.

    Directory of Open Access Journals (Sweden)

    Corinne Jud

    Full Text Available Over time, organisms developed various strategies to adapt to their environment. Circadian clocks are thought to have evolved to adjust to the predictable rhythms of the light-dark cycle caused by the rotation of the Earth around its own axis. The rhythms these clocks generate persist even in the absence of environmental cues with a period of about 24 hours. To tick in time, they continuously synchronize themselves to the prevailing photoperiod by appropriate phase shifts. In this study, we disrupted two molecular components of the mammalian circadian oscillator, Rev-Erbalpha and Period1 (Per1. We found that mice lacking these genes displayed robust circadian rhythms with significantly shorter periods under constant darkness conditions. Strikingly, they showed high amplitude resetting in response to a brief light pulse at the end of their subjective night phase, which is rare in mammals. Surprisingly, Cry1, a clock component not inducible by light in mammals, became slightly inducible in these mice. Taken together, Rev-Erbalpha and Per1 may be part of a mechanism preventing drastic phase shifts in mammals.

  1. Validation of CATHENA MOD-3.5/Rev0 for single-phase water hammer

    International Nuclear Information System (INIS)

    Beuthe, T.G.

    2000-01-01

    This paper describes work performed to validate the system thermalhydraulics code CATHENA MOD-3.5c/Rev0 for single-phase water hammer. Simulations were performed and are compared quantitatively against numerical tests and experimental results from the Seven Sisters Water Hammer Facility to demonstrate CATHENA can predict the creation and propagation of pressure waves when valves are opened and closed. Simulations were also performed to show CATHENA can model the behaviour of reflected and transmitted pressure waves at area changes, dead ends, tanks, boundary conditions, and orifices in simple and more complex piping systems. The CATHENA results are shown to calculate pressure and wave propagation speeds to within 0.2% and 0.5% respectively for numerical tests and within 3.3% and 5% for experimental results respectively. These results are used to help validate CATHENA for use in single-phase water hammer analysis. They also provide assurance that the fundamental parameters needed to successfully model more complex forms of water hammer are accounted for in the MOD-3.5c/Rev0 version of CATHENA, and represent the first step in the process to validate the code for use in modelling two-phase water hammer and condensation-induced water hammer. (author)

  2. Technical reports retrieval system(rev. 1) in the field of nuclear energy

    International Nuclear Information System (INIS)

    Choi, S.D.; Lee, Y.K.; Yim, S.H.

    1983-01-01

    TRRS(rev. 1), on-line Technical Reports Retrieval System, a set of computer programs, was designed and developed to provide fast and efficient access to computer-based information files. This system was foucused upon its application to the retrival of technical reports collected in KAERI, and developed not only to meet the requirements of researchers sitting at terminal but to accomodate its sufficiently general logic to other computer systems. The retrival program language is FORTRAN IV Plus. The users can search the whole files using next eleven TRRS(rev. 1) commands, HELP, SEARCH, LOOK, COMBINE, EXPAND, SELECT, REVIEW, RESTART, TYPE, PRINT, and END. The special features of this system are as follows. First, the SEARCH command can process full and truncation (truncation mark is %), and can combine such truncated terms using Boolean operators, and (*), and and-not (.). Second, COMBINE command can combine set numbers with year(s), language(s) and a substring of titles. Third, after EXPAND command, either full or truncated term, SELECT command brings same result of SEARCH command. Finally, real time response is very short, real time response is very short, usually within a second or less. (Author)

  3. Benchmark test of JENDL-3T and -3T/Rev.1

    International Nuclear Information System (INIS)

    Takano, Hideki; Kaneko, Kunio.

    1989-10-01

    The fast reactor 70-group constant set JFS-3-J3T has been generated by using the JENDL-3T nuclear data. One-dimensional 21-benchmark cores and the ZPPR-9 core were analysed with the JFS-3-J3T set. The results obtained are summarized as follows: (1) The values of keff are underestimated by 0.6% for Pu-fueled cores and overestimated by 2% for U-fueled cores. (2) The central reaction rate ratio 239 σ f φ/ 235 σ f φ is in a good agreement with the experimental value, though 238 σ c φ/ 239 σ f φ and 238 σ f φ/ 235 σ f φ are overestimated. (3) Doppler and Na-void reactivities are in a good agreement with the measured data. (4) The prediction accuracy of radial reaction rate distributions are improved in the comparison of the results obtained with the JENDL-2 data. Furthermore, the benchmark test of JENDL-3T/Rev. 1 which was revised from JENDL-3T for several important nuclides has been again performed. It was shown that JENDL-3T/Rev. 1 would predict nuclear characteristics more satisfactorily than JENDL-3T. (author)

  4. Seleção de progênies de erva-mate (Ilex paraguariensis St. Hill. para a produtividade, estabilidade e adaptabilidade temporal de massa foliar Selection of Erva-mate Progenies for Leaf Weight Productivity, Temporal Stability and Adaptability

    Directory of Open Access Journals (Sweden)

    José Alfredo Sturion

    2011-03-01

    Full Text Available O presente trabalho teve por objetivos selecionar progênies de erva-mate (Ilex paraguariensis St. Hil.  em Ivaí - PR, simultaneamente para estabilidade, adaptabilidade e produtividade de massa foliar,  colhidas em três safras, com base na média harmônica a performance relativa dos valores genéticos (MHPRVG, adicionalmente, comparar os resultados  com aqueles obtidos pelos métodos de Annicchiarico e  de Lin & Binns. O material genético consistiu de 25  progênies de meios-irmãos plantadas no delineamento  de blocos ao acaso, com dez repetições  e parcelas lineares de seis plantas no espaçamento de  3m x 2m. O peso de folhas foi avaliado, por árvore,  aos dois, quatro e seis anos após o plantio. Houve  uma alteração no ordenamento das progênies para a produção de massa foliar ao longo das safras. Os  métodos de Annicchiarico, Lin & Binns e MHPRVG  selecionaram, praticamente, as mesmas progênies para os três atributos considerados. Contudo, o método  HPRVG apresenta a vantagem de fornecer  resultados na própria escala e medição do caráter, os quais podem ser interpretados diretamente como  valores genéticos.
    This paper aimed to select progenies of “erva-mate” (Ilex paraguariensis St. HiI. in Ivai - PR based on leaf weight productivity, stability and adaptability across three harvests. This can be achieved by the method MHPRVG (harmonic average of relative performance of genetic values which is comparable with the methods by Annichiaricco and Lin & Binns. The genetic material was constituted by 25 half sib progenies evaluated in a complete block design with ten replicates and six plants per plot, in a 3 x 2 meters spacing. Leaf weight per tree  was evaluated at ages two, four and six years  after planting. There were some changes in progenies  ranking across harvests. The methods MHPRVG, Annichiaricco and Lin & Binns led to selection of the  same progenies, considering the three

  5. The structural basis of gas-responsive transcription by the human nuclear hormone receptor REV-ERBbeta.

    Directory of Open Access Journals (Sweden)

    Keith I Pardee

    2009-02-01

    Full Text Available Heme is a ligand for the human nuclear receptors (NR REV-ERBalpha and REV-ERBbeta, which are transcriptional repressors that play important roles in circadian rhythm, lipid and glucose metabolism, and diseases such as diabetes, atherosclerosis, inflammation, and cancer. Here we show that transcription repression mediated by heme-bound REV-ERBs is reversed by the addition of nitric oxide (NO, and that the heme and NO effects are mediated by the C-terminal ligand-binding domain (LBD. A 1.9 A crystal structure of the REV-ERBbeta LBD, in complex with the oxidized Fe(III form of heme, shows that heme binds in a prototypical NR ligand-binding pocket, where the heme iron is coordinately bound by histidine 568 and cysteine 384. Under reducing conditions, spectroscopic studies of the heme-REV-ERBbeta complex reveal that the Fe(II form of the LBD transitions between penta-coordinated and hexa-coordinated structural states, neither of which possess the Cys384 bond observed in the oxidized state. In addition, the Fe(II LBD is also able to bind either NO or CO, revealing a total of at least six structural states of the protein. The binding of known co-repressors is shown to be highly dependent upon these various liganded states. REV-ERBs are thus highly dynamic receptors that are responsive not only to heme, but also to redox and gas. Taken together, these findings suggest new mechanisms for the systemic coordination of molecular clocks and metabolism. They also raise the possibility for gas-based therapies for the many disorders associated with REV-ERB biological functions.

  6. The Structural Basis of Gas-Responsive Transcription by the Human Nuclear Hormone Receptor REV-ERBβ

    Science.gov (United States)

    Pardee, Keith I; Xu, Xiaohui; Reinking, Jeff; Schuetz, Anja; Dong, Aiping; Liu, Suya; Zhang, Rongguang; Tiefenbach, Jens; Lajoie, Gilles; Plotnikov, Alexander N; Botchkarev, Alexey; Krause, Henry M; Edwards, Aled

    2009-01-01

    Heme is a ligand for the human nuclear receptors (NR) REV-ERBα and REV-ERBβ, which are transcriptional repressors that play important roles in circadian rhythm, lipid and glucose metabolism, and diseases such as diabetes, atherosclerosis, inflammation, and cancer. Here we show that transcription repression mediated by heme-bound REV-ERBs is reversed by the addition of nitric oxide (NO), and that the heme and NO effects are mediated by the C-terminal ligand-binding domain (LBD). A 1.9 Å crystal structure of the REV-ERBβ LBD, in complex with the oxidized Fe(III) form of heme, shows that heme binds in a prototypical NR ligand-binding pocket, where the heme iron is coordinately bound by histidine 568 and cysteine 384. Under reducing conditions, spectroscopic studies of the heme-REV-ERBβ complex reveal that the Fe(II) form of the LBD transitions between penta-coordinated and hexa-coordinated structural states, neither of which possess the Cys384 bond observed in the oxidized state. In addition, the Fe(II) LBD is also able to bind either NO or CO, revealing a total of at least six structural states of the protein. The binding of known co-repressors is shown to be highly dependent upon these various liganded states. REV-ERBs are thus highly dynamic receptors that are responsive not only to heme, but also to redox and gas. Taken together, these findings suggest new mechanisms for the systemic coordination of molecular clocks and metabolism. They also raise the possibility for gas-based therapies for the many disorders associated with REV-ERB biological functions. PMID:19243223

  7. Variação fenotípica para caracteres silviculturais em populações de Aspidosperma spp. sem estrutura de progênies. Genetic variation for silvicultural traits in populations of Aspidosperma spp. without progeny arrays.

    Directory of Open Access Journals (Sweden)

    Camila Regina Silva Baleroni RECCO

    2016-06-01

    Full Text Available O objetivo deste estudo foi estimar os parâmetros genéticos para os caracteres silviculturais em Aspidosperma polyneuron Müll.Arg. e Aspidosperma cylindrocarpon Müll.Arg., para fins de conservação ex situ e uso em programas de recuperação ambiental. A coleta de sementes foi feita em árvores de polinização aberta no município de Andradina-SP e o teste experimental foi instalado na Fazenda de Ensino e Pesquisa (FEPE/UNESP, em 1997, com as espécies consorciadas com Inga marginata Willd. (ingá feijão em linhas alternadas. O delineamento experimental utilizado foi o de blocos casualizados completos com dois tratamentos (Aspidosperma spp., 32 repetições com parcelas lineares e 4 plantas por parcela. Aos 15 anos de idade foram mensurados diâmetro à altura do peito – DAP, altura de total – ALT e sobrevivência – SOB e os parâmetros genéticos foram estimados pelo programa Selegen. A espécie cylindrocarpon apresentou maior desenvolvimento nos caracteres de crescimento (DAP e ALT quando comparada com A. polyneuron. A taxa de sobrevivência foi de 71% demostrando boa adaptabilidade para ambas as espécies ao local de implantação do experimento. A acurácia foi alta, sugerindo maior confiabilidade das análises estatísticas. A herdabilidade foi moderada, indicando considerável controle genético nas espécies quando se observa que o coeficiente de determinação dentro de parcela foi menor que 10% para ALT e DAP, mostrando que as parcelas lineares são homogêneas, ou seja, o delineamento experimental adotado foi eficiente. Portanto, a ausência do uso de estrutura de progênies nas populações de Aspidosperma spp. estudadas não impossibilitou que fossem obtidas informações relevantes sobre as populações naturais de Aspidosperma, porém vale ressaltar que é de suma importância que novas coletas de sementes sejam realizadas para um estudo com base em teste de progênies. This study aimed to estimate genetic parameters

  8. Avaliação de produtividade de progênies de cafeeiro em dois sistemas de plantio Yield evaluation of coffee plant (Coffea arabica L. progenies in two tillage systems

    Directory of Open Access Journals (Sweden)

    Gladyston Rodrigues Carvalho

    2006-10-01

    Full Text Available Objetivou-se com o presente trabalho analisar a influência do sistema de plantio (adensado e convencional e a produtividade de cafeeiros (Coffea arabica L., resultantes do cruzamento de Catuaí Vermelho IAC 99 com Mundo Novo IAC 379-19. Foram utilizadas 29 progênies, na 4ª geração por autofecundação do 2º retrocruzamento de Catuaí Vermelho com Mundo Novo desenvolvidas pelo programa de Melhoramento Genético do Cafeeiro em Minas Gerais, coordenado pela EPAMIG. Utilizou-se como testemunha 13 cultivares, Topázio MG-1189 e 1189 SL, Catuaí Vermelho IAC-15, IAC-99 e IAC-144, Catuaí Amarelo MG-17, Rubi MG-1192 e 1192SL, Acaiá Cerrado MG-1474 e 1474 SL, Mundo Novo IAC379-19, IAC-376-4 e 376-4 SL. O experimento foi instalado na Fazenda Experimental da EPAMIG, em São Sebastião do Paraíso, MG em janeiro de 1996 no espaçamento de 3,50 x 1,00 m (sistema convencional e 1,80 x 0,60 m (sistema adensado. O delineamento experimental foi em blocos casualizados, sendo os tratamentos compostos por 42 progênies/cultivares com três repetições e seis plantas por parcela com a análise de variância, em parcelas subdivididas, onde cada biênio (agrupamento de colheita foi considerado como uma subparcela. Avaliou-se a produção de grãos em sacas de 60 kg de café beneficiado/ha. Verificou-se nos resultados que a produtividade inicial no sistema adensado foi maior que no sistema convencional, porém com base no desenvolvimento das plantas essa diferença tendeu a diminuir. Para ambos os sistemas de plantio, pode-se utilizar qualquer uma das cultivares uma vez que as mesmas comportaram-se de forma semelhante. As cultivares utilizadas como testemunha que apresentaram as maiores produtividades foram Topázio MG-1189 e 1189 SL, Rubi MG-1192 e 1192SL, Catuaí Vermelho IAC 15 e IAC 144 e Mundo Novo IAC-376-4 e 376-4SL.The present work was carried out in order to analyze the planting system (high and low plant stand influence on coffee trees (Coffea

  9. A high-resolution map of the Grp1 locus on chromosome V of potato harbouring broad-spectrum resistance to the cyst nematode species Globodera pallida and Globodera rostochiensis.

    Science.gov (United States)

    Finkers-Tomczak, Anna; Danan, Sarah; van Dijk, Thijs; Beyene, Amelework; Bouwman, Liesbeth; Overmars, Hein; van Eck, Herman; Goverse, Aska; Bakker, Jaap; Bakker, Erin

    2009-06-01

    The Grp1 locus confers broad-spectrum resistance to the potato cyst nematode species Globodera pallida and Globodera rostochiensis and is located in the GP21-GP179 interval on the short arm of chromosome V of potato. A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes. The flanking markers GP21 and GP179 have been used to screen the 1,536 genotypes for recombination events. Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432. Detailed analysis of the 44 recombinant genotypes showed that G. pallida and G. rostochiensis resistance could not be separated and map to the same location between marker SPUD838 and TG432. It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

  10. REV-ERB-ALPHA circadian gene variant associates with obesity in two independent populations: Mediterranean and North American.

    Science.gov (United States)

    Garaulet, Marta; Smith, Caren E; Gomez-Abellán, Purificación; Ordovás-Montañés, María; Lee, Yu-Chi; Parnell, Laurence D; Arnett, Donna K; Ordovás, José M

    2014-04-01

    Despite the solid connection between REV-ERB and obesity, the information about whether genetic variations at this locus may be associated with obesity traits is scarce. Therefore our objective was to study the association between REV-ERB-ALPHA1 rs2314339 and obesity in two independent populations. Participants were 2214 subjects from Spanish Mediterranean (n = 1404) and North American (n = 810) populations. Anthropometric, biochemical, dietary, and genotype analyses were performed. We found novel associations between the REV-ERB-ALPHA1 rs2314339 genotype and obesity in two independent populations: in Spanish Mediterranean and North American groups, the frequency of the minor-allele-carriers (AA+ AG) was significantly lower in the "abdominally obese" group than in those of the "nonabdominally obese" group (p obesity than noncarriers, and the effect was of similar magnitude for both populations (OR ≈ 1.50). There were consistent associations between REV-ERB-ALPHA1 genotype and obesity-related traits (p obesity was also detected in the Mediterranean population. This new discovery highlights the importance of REV-ERB-ALPHA1 in obesity and provides evidence for the connection between our biological clock and obesity-related traits. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Administrative Circular No. 2 (Rev. 7) - Recruitment, appointment and possible developments regarding the contractual position of staff members

    CERN Multimedia

    2015-01-01

    Administrative Circular No. 2 (Rev. 7), entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members", approved by the Director-General following discussion at the Standing Concertation Committee meeting held on 17 February 2015 is available via the following link: AC No. 2 (Rev.7).   This revised circular cancels and replaces Administrative Circular No. 2 (Rev. 6), entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members" and dated January 2015. The circular was revised in order to implement the amendment to Article R II 1.17 of the Staff Regulations, which introduces the possibility of extending limited-duration (LD) contracts up to a maximum total duration of eight years from the previous duration of five years. The award of indefinite contracts will continue to be subject to the outcome of a competitive process. Department Head Of...

  12. Administrative Circular No. 30 (Rev. 3) - “Financial benefits on taking up appointment and on termination of contract”

    CERN Multimedia

    2013-01-01

    Administrative Circular No. 30 (Rev. 3) entitled “Financial benefits on taking up appointment and on termination of contract”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 27 June 2013 and entering into force on 1 August 2013, is available on the intranet site of the Human Resources Department (see here).   Administrative Circular No. 30 (Rev. 3) is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 30 (Rev. 2/Corr.) entitled "Financial benefits on taking up appointment and on termination of contract” of September 2009. This circular was revised in order to implement the modifications introduced into the Staff Rules and Regulations in January 2013 relating to the introduction of the status of Associate Member States and new categories of associated members of the personnel. In particular, the notion of “Member State” in Annexe II (&a...

  13. Administrative Circulars Nos. 12 A and B (Rev. 1) – Education Fees

    CERN Multimedia

    HR Department

    2007-01-01

    Administrative Circulars Nos. 12 A and B (Rev. 1) – "Education Fees" are now available on the intranet site of the Human Resources Department: http://cern.ch/hr-docs/admincirc/admincirc.asp These Circulars cancel and replace Administrative Circular No.12 – "Education Grant" of April 1981. Copies will shortly be available in Departmental secretariats. Administrative Circular No. 12 A is applicable to staff members (except former "local staff"), fellows and paid associates, recruited before 1st January 2007. It may be noted that, at the initiative of the Human Resources Department, a number of important simplifications have been introduced. These cover in particular lump sum payments to compensate for accommodation, meals and journey expenses. Administrative Circular No. 12 B is applicable to staff members, fellows, scientific associates recruited as of 1st January 2007, as well as to former "local staff" recruited prior to this date. If you require any additional informa...

  14. Administrative Circulars Nos. 12 A and B (Rev. 1) – Education Fees

    CERN Multimedia

    HR Department

    2007-01-01

    Administrative Circulars Nos. 12 A and B (Rev. 1) – "Education Fees" are now available on the intranet site of the Human Resources Department: http://https://hr-docs.web.cern.ch/hr-docs/admincirc/admincirc_fr.asp These circulars cancel and replace Administrative Circular No.12 – "Education Grant" of April 1981. Copies will shortly be available in Departmental secretariats. Administrative Circular No. 12 A is applicable to staff members (except former "local staff"), fellows and paid associates, recruited before 1st January 2007. It may be noted that, at the initiative of the Human Resources Department, a number of important simplifications have been introduced. These cover, in particular, lump sum payments to compensate for accommodation, meals and journey expenses. Administrative Circular No. 12 B is applicable to staff members, fellows, scientific associates recruited as of 1st January 2007, as well as to former "local staff" recruited prior to this date. If you requi...

  15. Safeguards Summary Event List (SSEL), January 1, 1990--December 31, 1996, Vol. 2, Rev. 5

    International Nuclear Information System (INIS)

    1997-07-01

    The Safeguards Summary Event List (SSEL), Vol. 2, Rev. 5, provides brief summaries of several hundred safeguards-related events involving nuclear material or facilities regulated by the US Nuclear Regulatory Commission (NRC) which occurred and were reported from January 1, 1990, through December 31, 1996. Because of public interest, the Miscellaneous category includes a few events which involve either source material, byproduct material, or natural uranium which are exempt from safeguards requirements. Events are described under the categories of Bomb-related, Intrusion, Missing and/or Allegedly Stolen, Transportation-related, Tampering/Vandalism, Arson, Firearms, Radiological Sabotage, Nonradiological Sabotage, and Miscellaneous. The information contained in the event descriptions is derived primarily from official NRC reporting channels

  16. Numerical Modelling of Large-Diameter Steel Piles at Horns Rev

    DEFF Research Database (Denmark)

    Augustesen, Anders Hust; Brødbæk, K. T.; Møller, M.

    2009-01-01

    Today large-diameter monopiles are the most common foundation type used for large offshore wind farms. This paper aims to investigate the behaviour of monopiles under monotonic loading taking the interaction between the pile and the subsoil into account. Focus is paid to a monopile used as founda......Today large-diameter monopiles are the most common foundation type used for large offshore wind farms. This paper aims to investigate the behaviour of monopiles under monotonic loading taking the interaction between the pile and the subsoil into account. Focus is paid to a monopile used...... as foundation for a wind turbine at Horns Rev located in the Danish sector of the North Sea. The outer diameter of the pile is 4 m and the subsoil at the location consists primarily of sand. The behaviour of the pile is investigated under realistic loading conditions by means of a traditional Winkler...

  17. Safeguards Summary Event List (SSEL), January 1, 1990--December 31, 1996, Vol. 2, Rev. 5

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    The Safeguards Summary Event List (SSEL), Vol. 2, Rev. 5, provides brief summaries of several hundred safeguards-related events involving nuclear material or facilities regulated by the US Nuclear Regulatory Commission (NRC) which occurred and were reported from January 1, 1990, through December 31, 1996. Because of public interest, the Miscellaneous category includes a few events which involve either source material, byproduct material, or natural uranium which are exempt from safeguards requirements. Events are described under the categories of Bomb-related, Intrusion, Missing and/or Allegedly Stolen, Transportation-related, Tampering/Vandalism, Arson, Firearms, Radiological Sabotage, Nonradiological Sabotage, and Miscellaneous. The information contained in the event descriptions is derived primarily from official NRC reporting channels.

  18. Numerical modelling of powder caking at REV scale by using DEM

    Science.gov (United States)

    Guessasma, Mohamed; Silva Tavares, Homayra; Afrassiabian, Zahra; Saleh, Khashayar

    2017-06-01

    This work deals with numerical simulation of powder caking process caused by capillary condensation phenomenon. Caking consists in unwanted agglomeration of powder particles. This process is often irreversible and not easy to predict. To reproduce mechanism involved by caking phenomenon we have used the Discrete Elements Method (DEM). In the present work, we mainly focus on the role of capillary condensation and subsequent liquid bridge formation within a granular medium exposed to fluctuations of ambient relative humidity. Such bridges cause an attractive force between particles, leading to the formation of a cake with intrinsic physicochemical and mechanical properties. By considering a Representative Elementary Volume (REV), the DEM is then performed by means of a MULTICOR-3D software tacking into account the properties of the cake (degree of saturation) in order to establish relationships between the microscopic parameters and the macroscopic behaviour (tensile strength).

  19. Numerical modelling of powder caking at REV scale by using DEM

    Directory of Open Access Journals (Sweden)

    Guessasma Mohamed

    2017-01-01

    Full Text Available This work deals with numerical simulation of powder caking process caused by capillary condensation phenomenon. Caking consists in unwanted agglomeration of powder particles. This process is often irreversible and not easy to predict. To reproduce mechanism involved by caking phenomenon we have used the Discrete Elements Method (DEM. In the present work, we mainly focus on the role of capillary condensation and subsequent liquid bridge formation within a granular medium exposed to fluctuations of ambient relative humidity. Such bridges cause an attractive force between particles, leading to the formation of a cake with intrinsic physicochemical and mechanical properties. By considering a Representative Elementary Volume (REV, the DEM is then performed by means of a MULTICOR-3D software tacking into account the properties of the cake (degree of saturation in order to establish relationships between the microscopic parameters and the macroscopic behaviour (tensile strength.

  20. OPERATIONAL CIRCULAR No. 4 (REV. 1) – USE OF VEHICLES BELONGING TO OR RENTED BY CERN

    CERN Document Server

    2012-01-01

    Operational Circular No. 4 (Rev. 1) entitled “Use of vehicles belonging to or rented by CERN”, approved by the Director-general following discussion in the Standing Concertation Committee meeting of 15 February 2012, is available on the intranet site of the Human Resources Department: https://hr-docs.web.cern.ch/hr-docs/opcirc/opcirc.asp It cancels and replaces Operational Circular No. 4 entitled “Conditions for use by members of the CERN personnel of vehicles belonging to or rented by CERN” of April 2003. This new version enables, in particular, to include CERN contractors and their personnel, to harmonize the structure of the circular with other circulars and to simplify the procedures by permitting electronics forms. Department Head Office HR Department

  1. Introduction Of Wavestar Wave Energy Converters At The Danish Offshore Wind Power Plant Horns Rev 2

    DEFF Research Database (Denmark)

    Marquis, L.; Kramer, Morten; Kringelum, J.

    is to be connected to a wind turbine at the DONG Energy owned wind power plant Horns Rev 2 placed off the western coast of Denmark. The plant delivers its energy production to a transformer station owned by Energinet.dk. Energinet.dk has the obligation to ensure that power is transmitted to the Danish consumers...... with this combination. This can increase the value of the produced power from future wind/wave plants. Further potential synergies of combining wind and wave energy in the same area include increased energy production from the available area and sharing of infrastructure costs as well as O&M facilities. In a future....... If Executed the project will be the first one in the world where wind and wave power are combined at full scale. The goal of the project is to evaluate the opportunities of combining wind and wave energy production on a commercial scale and to demonstrate the reduction of energy fluctuations...

  2. Hard bottom substate monitoring Horns Rev offshore wind farm 2004. Survey report no. 1

    Energy Technology Data Exchange (ETDEWEB)

    Frederiksen, Rune

    2004-05-15

    In connection with the implementation of the monitoring programme concerning the ecological implication of the effect of the introduction of hard substrate related to the Horns Rev Wind Farm, the third survey was carried out in the period 2431 March 2004. The survey covered collection of fauna and flora samples from the scour protection and at the wind turbine towers at six turbine sites. Video recordings were planned at different sites to provide documentation, but due to poor visibility the video recordings were postponed. On request from Elsam Engineering A/S video inspections on technical installations were made at turbine '84 and 95. This report covers a short description of the methodology, sampling activities and site description. (au)

  3. Avaliação e seleção de progênies F3 de cafeeiros de porte baixo com o gene SH3 de resistência a Hemileia vastatrix Berk. et Br. Evaluation and selection of Coffea arabica F3 progenies with low height and the leaf-rust SH3 resistence gene

    Directory of Open Access Journals (Sweden)

    Albano Silva da Conceição

    2005-01-01

    Full Text Available Com o objetivo de avaliar e selecionar progênies F3 de cafeeiros de porte baixo com o gene SH3 de resistência à ferrugem, foram estudadas 36 progênies de cafeeiros tipo arábica (Coffea arabica L. , em geração F3, resultantes dos cruzamentos dirigidos entre as cultivares Catuaí Vermelho IAC 46 e Catuaí Vermelho IAC 81 com o acesso IAC 1110 (BA-10. Esse último, originário da Índia, é fonte dos genes SH2SH3 que conferem resistência a Hemileia vastatrix. O experimento, estabelecido em 1988 no Centro Experimental do Instituto Agronômico, em Campinas (SP, no delineamento experimental em blocos ao acaso com seis repetições, duas plantas por parcela e no espaçamento 3,0 x 1,8 m, utilizou como testemunha a cultivar Catuaí Vermelho IAC 81, totalizando 37 tratamentos. Avaliaram-se no campo, a produção de café (média de sete colheitas, vigor vegetativo, resistência à ferrugem, porte da planta, coloração das folhas novas e maturação dos frutos. Os frutos das plantas mais produtivas foram analisados em laboratório quanto ao rendimento, tipos de sementes, peneira média e massa de 1000 grãos. A análise da variância dos dados de produção das progênies evidenciou que houve diferenças significativas entre as progênies, ao nível de 1% de probabilidade, pelo teste F. Foram selecionadas 11 progênies com média superior à testemunha e dentro dessas, 39 cafeeiros. Das 25 progênies restantes foram selecionados mais 15 cafeeiros produtivos e resistentes ao agente da ferrugem. Desses 54 cafeeiros, foram selecionados os 18 que apresentaram peneira média acima de 15,5 e maior freqüência de grãos normais do tipo chato. As progênies dessas plantas selecionadas foram avaliadas na geração F4, em fase de mudas, quando se verificou que dez delas estavam em homozigoze para porte baixo. Com as 18 plantas, o Programa de Melhoramento do Café, no IAC, terá continuidade como progênies F4, visando à obtenção de nova cultivar de

  4. Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactors

    International Nuclear Information System (INIS)

    Kiss, A.; Li, L.; Gettemeier, T.; Venkatesh, L.K.

    2003-01-01

    Human immunodeficiency virus type 1 (HIV-1) Rev-mediated nuclear export of viral RNAs involves the interaction of its leucine-rich nuclear export sequence (NES) with nuclear cofactors. In yeast two-hybrid screens of a human lymph node derived cDNA expression library, we identified the human nucleoporin Nup98 as a highly specific and potent interactor of the Rev NES. Using an extensive panel of nuclear export positive and negative mutants of the functionally homologous NESs of the HIV-1 Rev, human T cell leukemia virus type 1 (HTLV-1) Rex, and equine infectious anemia virus (EIAV) Rev proteins, physiologically significant interaction of hNup98 with the various NESs was demonstrated. Missense mutations in the yeast nuclear export factor Crm1p that abrogated Rev NES interaction with the XXFG repeat-containing nucleoporin, Rab/hRIP, had minimal effects on the interaction of GLFG repeat-containing hNup98. Functional analysis of Nup98 domains required for nuclear localization demonstrated that the entire ORF was required for efficient incorporation into the nuclear envelope. A putative nuclear localization signal was identified downstream of the GLFG repeat region. Whereas overexpression of both full-length Nup98 and the amino-terminal GLFG repeat region, but not the unique carboxy-terminal region, induced significant suppression of HIV unspliced RNA export, lower levels of exogenous Nup98 expression resulted in a relatively modest increase in unspliced RNA export. These results suggest a physiological role for hNup98 in modulating Rev-dependent RNA export during HIV infection

  5. Avaliação de progênies de macaúba na fase juvenil e estimativas de parâmetros genéticos e diversidade genética Evaluation of macaw palm progenies in juvenile phase and estimates of genetic parameters and genetic diversity

    Directory of Open Access Journals (Sweden)

    Candida Elisa Manfio

    2012-03-01

    Full Text Available

    A macaúba (Acrocomia aculeata é uma palmeira oleaginosa e o extrativismo de seus frutos é de grande importância socioeconômica para os pequenos produtores rurais. O objetivo deste trabalho foi avaliar a variabilidade genética e obter informações sobre o controle genético de características morfológicas relacionadas ao crescimento e vigor da macaúba. Foram coletadas 51 progênies em Minas Gerais e São Paulo, as quais são integrantes do banco ativo de germoplasma da Universidade Federal de Viçosa. Doze meses após o plantio, essas progênies foram avaliadas quanto ao crescimento da planta (cm e número de folhas emitidas. Estas características apresentaram grande variabilidade entre as progênies. As estimativas dos coeficientes de herdabilidade foram de 87% e 48%, para crescimento de planta e número de folhas, respectivamente, revelando controle genético e boas perspectivas para o melhoramento. O método de Tocher mostrou-se eficiente em alocar as progênies do Estado de São Paulo em um mesmo grupo. O método de seleção pelo Índice de rank médio permitiu selecionar indivíduos e progênies mais equilibrados em termos dos dois caracteres.

     

    doi: 10.4336/2012.pfb.32.69.63

    The macaw palm (Acrocomia aculeate is an extraction oil palm and its fruit is of great socioeconomic importance for small farmers. The aim of this study was to evaluate the genetic variability and to obtain information about the genetic control of morphological traits related to growth and vigor of macaw palm. Fifty-one open pollinated progenies were collected in Minas Gerais and São Paulo States, which are active members of the germplasm bank of Viçosa Federal University. These 51 progenies, twelve months after planting, were evaluated for plant growth and number of leaves emitted. The characteristics evaluated presented great variability among the progenies. Heritability coefficient of 87% and 48% were

  6. Predição de parâmetros e valores genéticos para caracteres de crescimento e produção de látex em progênies de seringueira Genetic parameters and values prediction for growth and latex production traits in rubber tree progenies

    Directory of Open Access Journals (Sweden)

    Reginaldo Brito da Costa

    2010-01-01

    Full Text Available O trabalho objetivou estimar parâmetros e valores genéticos para os caracteres altura, diâmetro, produção de látex e produtividade de progênies de seringueira. As progênies meio-irmãos foram estabelecidas sob delineamento de blocos ao acaso, com 28 tratamentos (progênies, cinco repetições e dez plantas por parcela. Aos três anos de idade, as progênies foram avaliadas quanto aos caracteres: a altura total (cm; b diâmetro (mm; c Produção de borracha seca (g e produtividade (g cm-2. As estimativas dos coeficientes de herdabilidades individuais (0,33; 0,24 e 0,51 para os caracteres altura, diâmetro e produtividade, respectivamente, foram consideradas expressivas. Para o caráter produção de borracha seca, a estimativa do coeficiente de herdabilidade individual, no sentido restrito (9%, embora de baixa magnitude, revela excelente possibilidade de seleção, pois conduziram a estimativa da herdabilidade, em nível de médias de família, a um valor equivalente a 68%. A acurácia entre os valores genéticos preditos e os verdadeiros foram de 0,80 para altura, 0,76 para diâmetro, 0,62 para produção de borracha seca e 0,86 para produtividade. As estimativas de herdabilidades individuais associadas às de médias de progênies podem maximizar os ganhos genéticos com a seleção na população. A inclusão do caráter produtividade foi promissora e deve ser utilizada na seqüência das avaliações, subsidiando o programa de melhoramento genético da espécie no Estado de Mato Grosso do Sul.This study aimed to estimate genetic values and parameters for plant height, diameter, rubber yield and productivity (g cm-2 of rubber tree progenies. Half-sib progenies were established in a complete randomized block, with 28 treatments, five replicates and 10 plants per plot. At three years of life, the progenies were evaluated regarding the following characters: diameter, rubber production and productivity (g cm². The estimative coefficients

  7. A stably expressed llama single-domain intrabody targeting Rev displays broad-spectrum anti-HIV activity.

    Science.gov (United States)

    Boons, Eline; Li, Guangdi; Vanstreels, Els; Vercruysse, Thomas; Pannecouque, Christophe; Vandamme, Anne-Mieke; Daelemans, Dirk

    2014-12-01

    The HIV Rev protein mediates the transport of partially and unspliced HIV mRNA from the nucleus to the cytoplasm. Rev multimerizes on a secondary stem-loop structure present in the viral intron-containing mRNA species and recruits the cellular karyopherin CRM1 to export viral mRNAs from the nucleus to the cytoplasm. Previously we have identified a single-domain intrabody (Nb(190)), derived from a llama heavy-chain antibody, which efficiently inhibits Rev multimerization and suppresses the production of infectious virus. We recently mapped the epitope of this nanobody and demonstrated that Rev residues K20 and Y23 are crucial for interaction while residues V16, H53 and L60 are important to a lesser extent. Here, we generated cell lines stably expressing Nb(190) and assessed the capacity of these cell lines to suppress the replication of different HIV-1 subtypes. These cells stably expressing the single-domain antibody are protected from virus-induced cytopathogenic effect even in the context of high multiplicity of infection. In addition, the replication of different subtypes of group M and one strain of group O is significantly suppressed in these cell lines. Next, we analysed the natural variations of Rev amino acids in sequence samples from HIV-1 infected patients worldwide and assessed the effect of Nb(190) on the most prevalent polymorphisms occurring at the key epitope positions (K20 and Y23) in Rev. We found that Nb(190) was able to suppress the function of these Rev variants except for the K20N mutant, which was present in only 0.7% of HIV-1 sequence populations (n = 4632). Cells stably expressing the single-domain intrabody Nb(190) are protected against virus-induced cytopathogenic effect and display a selective survival advantage upon infection. In addition, Nb(190) suppresses the replication of a wide range of different HIV-1 subtypes. Large-scale sequence analysis reveals that the Nb(190) epitope positions in Rev are well conserved across major HIV-1

  8. Administrative circular No. 2 (Rev. 5) – Recruitment, appointment and possible developments regarding the contractual position of staff members

    CERN Document Server

    HR Department

    2011-01-01

    Administrative Circular No. 2 (Rev. 5) entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 1 September 2011, is available on the intranet site of the Human Resources Department: https://cern.ch/hr-docs/admincirc/admincirc.asp It cancels and replaces Administrative Circular No. 2 (Rev. 4) entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members" of September 2009. Department Head Office

  9. Persistence of attenuated HIV-1 rev alleles in an epidemiologically linked cohort of long-term survivors infected with nef-deleted virus

    Directory of Open Access Journals (Sweden)

    Wesselingh Steven L

    2007-07-01

    Full Text Available Abstract Background The Sydney blood bank cohort (SBBC of long-term survivors consists of multiple individuals infected with nef-deleted, attenuated strains of human immunodeficiency virus type 1 (HIV-1. Although the cohort members have experienced differing clinical courses and now comprise slow progressors (SP as well as long-term nonprogressors (LTNP, longitudinal analysis of nef/long-terminal repeat (LTR sequences demonstrated convergent nef/LTR sequence evolution in SBBC SP and LTNP. Thus, the in vivo pathogenicity of attenuated HIV-1 strains harboured by SBBC members is dictated by factors other than nef/LTR. Therefore, to determine whether defects in other viral genes contribute to attenuation of these HIV-1 strains, we characterized dominant HIV-1 rev alleles that persisted in 4 SBBC subjects; C18, C64, C98 and D36. Results The ability of Rev derived from D36 and C64 to bind the Rev responsive element (RRE in RNA binding assays was reduced by approximately 90% compared to Rev derived from HIV-1NL4-3, C18 or C98. D36 Rev also had a 50–60% reduction in ability to express Rev-dependent reporter constructs in mammalian cells. In contrast, C64 Rev had only marginally decreased Rev function despite attenuated RRE binding. In D36 and C64, attenuated RRE binding was associated with rare amino acid changes at 3 highly conserved residues; Gln to Pro at position 74 immediately N-terminal to the Rev activation domain, and Val to Leu and Ser to Pro at positions 104 and 106 at the Rev C-terminus, respectively. In D36, reduced Rev function was mapped to an unusual 13 amino acid extension at the Rev C-terminus. Conclusion These findings provide new genetic and mechanistic insights important for Rev function, and suggest that Rev function, not Rev/RRE binding may be rate limiting for HIV-1 replication. In addition, attenuated rev alleles may contribute to viral attenuation and long-term survival of HIV-1 infection in a subset of SBBC members.

  10. Analysis of translesion DNA synthesis activity of the human REV1 protein, which is a key player in radiation-induced mutagenesis

    International Nuclear Information System (INIS)

    Masuda, Yuji; Kamiya, Kenji

    2003-01-01

    Ionizing radiation frequently causes oxidative DNA damage in cells. It has been suggested that functions of the REV1 gene are induction of mutations and prevention of cell death caused by ionizing radiation through the damage bypass DNA replication. The gene product possesses a deoxycytidyl transferase activity, which is required for translesion DNA synthesis of a variety of damaged bases and an abasic site. To elucidate molecular mechanisms of the mutagenesis and translesion DNA synthesis, it is important to characterize the enzymatic properties of the REV1 protein. Here, we describe a novel method for purifying the recombinant human REV1 protein and the anzymatic properties of the protein. We established an efficient system for induction of the recombinant human REV1 protein in Escherichia coli cells. The REV1 protein was purified to homogeneity using nickel-chelating sepharose, heparin sepharose and superdex 200 chromatography. When purified by this method, REV1 protein is free of endo-, exonuclease and DNA polymerase activities. The purified REV1 protein is suitable for enzymological studies, and we used this to biochemical characterization. The REV1 protein inserts dCMP opposite templates G, A, T, C and an abasic site and inserts dGMP and dTMP opposite template G. Kinetic analysis provided evidence for high efficiency for dCMP insertion opposite template G and an abasic site, suggesting that the REV1 protein play a role in translesion DNA synthesis of an abasic site. (author)

  11. OPERATIONAL CIRCULAR NO2 (REV. 1) - APRIL 1998 'CONDITIONS OF ACCESS TO THE FENCED PARTS OF THE CERN SITE'

    CERN Multimedia

    Human Resources Division

    2001-01-01

    Subsidiary document 'Implementation Measures' (Rev. 2) - April 2001 The subsidiary document has been amended. Copies are available from Divisional Secretariats and at CERN card issue points. Note : Administrative and operational circulars, as well as the lists of those in force, are available for consultation on WWW : ADMINISTRATIVE CIRCULARS OPERATIONAL CIRCULARS

  12. 78 FR 27971 - Determination That REV-EYES (Dapiprazole Hydrochloride Ophthalmic Solution), 0.5%, Was Not...

    Science.gov (United States)

    2013-05-13

    ...] Determination That REV-EYES (Dapiprazole Hydrochloride Ophthalmic Solution), 0.5%, Was Not Withdrawn From Sale... ophthalmic solution), 0.5%, was not withdrawn from sale for reasons of safety or effectiveness. This... ophthalmic solution, 0.5%, if all other legal and regulatory requirements are met. FOR FURTHER INFORMATION...

  13. Finanšu pārskata revīzijas process lielai sabiedrībai

    OpenAIRE

    Špoģe, Sarmīte

    2018-01-01

    Finanšu pārskata revīzijas process ir svarīgs temats, jo pirmreizēji revidētiem uzņēmumiem tiek atklātas būtiskas neatbilstības grāmatvedības uzskaitē, tās atbilstībai LR likumiem un normatīvajiem aktiem, kā arī finanšu pārskata sagatavošanā. Darba mērķis: apkopot un analizēt finanšu pārskata revīzijas procesu lielā sabiedrībā, noteikt revīzijas riskus, izstrādāt priekšlikumus revīzijas procesa uzlabošanai. Izvirzīto mērķu sasniegšanai autore pētīja un apkopoja informāciju par finanšu pārska...

  14. Upper Bound Radiation Dose Assessment for Military Personnel at McMurdo Station, Antarctica, between 1962 and 1979 (2REV)

    Science.gov (United States)

    2017-09-30

    N IC A L R EP O R T Defense Threat Reduction Agency 8725 John J. Kingman Road , MS-6201 Fort Belvoir, VA 22060-6201 STANDARD FORM 298 (Rev. 8/98...8725 John J. Kingman Road , Mail Stop 6201 Fort...Early Operation ..................................................................................... 5 2.2 Operations and Maintenance

  15. Rev1 contributes to proper mitochondrial function via the PARP-NAD(+)-SIRT1-PGC1 alpha axis

    DEFF Research Database (Denmark)

    Fakouri, Nima Borhan; Durhuus, Jon Ambaek; Regnell, Christine Elisabeth

    2017-01-01

    (ADP) ribose polymerase 1 (PARP1) activity, low endogenous NAD+, low expression of SIRT1 and PGC1α and low adenosine monophosphate (AMP)-activated kinase (AMPK) activity. We conclude that replication stress via Rev1-deficiency contributes to metabolic stress caused by compromized mitochondrial function via...... the PARP-NAD+-SIRT1-PGC1α axis....

  16. Reliability of Concentric, Eccentric and Isometric Knee Extension and Flexion when using the REV9000 Isokinetic Dynamometer

    DEFF Research Database (Denmark)

    de Carvalho Froufe Andrade, Alberto César Pereira; Caserotti, Paolo; de Carvalho, Carlos Manuel Pereira

    2013-01-01

    The aim of this study was to assess the reliability of isokinetic and ISO knee extensor and flexor muscle strength when using the REV9000 (Technogym) isokinetic dynamometer. Moreover, the reliability of several strength imbalance indices and bilateral ratios were also examined. Twenty-four physic...

  17. A Comprehensive Experiment for Molecular Biology: Determination of Single Nucleotide Polymorphism in Human REV3 Gene Using PCR-RFLP

    Science.gov (United States)

    Zhang, Xu; Shao, Meng; Gao, Lu; Zhao, Yuanyuan; Sun, Zixuan; Zhou, Liping; Yan, Yongmin; Shao, Qixiang; Xu, Wenrong; Qian, Hui

    2017-01-01

    Laboratory exercise is helpful for medical students to understand the basic principles of molecular biology and to learn about the practical applications of molecular biology. We have designed a lab course on molecular biology about the determination of single nucleotide polymorphism (SNP) in human REV3 gene, the product of which is a subunit of…

  18. The calculational VVER burnup Credit Benchmark No.3 results with the ENDF/B-VI rev.5 (1999)

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Gual, Maritza [Centro de Tecnologia Nuclear, La Habana (Cuba). E-mail: mrgual@ctn.isctn.edu.cu

    2000-07-01

    The purpose of this papers to present the results of CB3 phase of the VVER calculational benchmark with the recent evaluated nuclear data library ENDF/B-VI Rev.5 (1999). This results are compared with the obtained from the other participants in the calculations (Czech Republic, Finland, Hungary, Slovaquia, Spain and the United Kingdom). The phase (CB3) of the VVER calculation benchmark is similar to the Phase II-A of the OECD/NEA/INSC BUC Working Group benchmark for PWR. The cases without burnup profile (BP) were performed with the WIMS/D-4 code. The rest of the cases have been carried with DOTIII discrete ordinates code. The neutron library used was the ENDF/B-VI rev. 5 (1999). The WIMS/D-4 (69 groups) is used to collapse cross sections from the ENDF/B-VI Rev. 5 (1999) to 36 groups working library for 2-D calculations. This work also comprises the results of CB1 (obtained with ENDF/B-VI rev. 5 (1999), too) and CB3 for cases with Burnup of 30 MWd/TU and cooling time of 1 and 5 years and for case with Burnup of 40 MWd/TU and cooling time of 1 year. (author)

  19. Epitopes of human immunodeficiency virus regulatory proteins tat, nef, and rev are expressed in normal human tissue

    NARCIS (Netherlands)

    Parmentier, H. K.; van Wichen, D. F.; Meyling, F. H.; Goudsmit, J.; Schuurman, H. J.

    1992-01-01

    The expression of regulatory proteins tat, rev, and nef of human immunodeficiency virus type-1 (HIV-1) and tat of HIV-2 was studied in frozen sections of lymph nodes from HIV-1-infected individuals, and various tissues from uninfected persons. In HIV-1-positive lymph nodes, monoclonal antibodies to

  20. Control of HIV replication in astrocytes by a family of highly conserved host proteins with a common Rev-interacting domain (Risp).

    Science.gov (United States)

    Vincendeau, Michelle; Kramer, Susanne; Hadian, Kamyar; Rothenaigner, Ina; Bell, Jeanne; Hauck, Stefanie M; Bickel, Christian; Nagel, Daniel; Kremmer, Elisabeth; Werner, Thomas; Leib-Mösch, Christine; Brack-Werner, Ruth

    2010-10-23

    In human astrocytes, restriction of HIV replication involves inhibition of HIV Rev activity. We previously identified a Rev-interacting human protein fragment (16.4.1) that can reduce Rev activity. The 16.4.1 sequence is contained in a group of highly similar host cell proteins, which we call the Risp family. Here we investigate whether the Risp family is connected to HIV replication in astrocytes. Cell/tissue lysates were analyzed for Risp expression by western blot with various anti-Risp antibodies. The interaction of astrocytic Risp members with Rev was investigated by affinity chromatography. Astrocytes were transfected with expression plasmids containing cDNAs encoding full-length Risp or the isolated 16.4.1 region for Risp overexpression or with siRNAs designed for Risp knock-down. Rev activity was investigated with a Rev-reporter assay. RNA levels were quantified by real-time RT-PCR, HIV Gag levels by p24ELISA. Expression of the Risp family was demonstrated in human brain tissues and astrocytes. Astrocytes were shown to produce Risp family members that interact with Rev. Production of HIV Gag proteins and Rev-dependent RNAs in persistently infected astrocytes increased upon Risp knock-down and decreased upon Risp overexpression. Risp knock-down increased Rev activity and raised proportions of Rev proteins in the nucleus of astrocytes. Our results link the Risp family to restriction of HIV production and inhibition of Rev activity in astrocytes. We conclude that the Risp family represents a novel family of host factors that can control HIV replication and may be important for the containment of HIV infection in brain reservoirs.

  1. Study on the REV Size of Fractured Rock in the Non-Darcy Flow Based on the Dual-Porosity Model

    Directory of Open Access Journals (Sweden)

    Yuan Wang

    2018-01-01

    Full Text Available For the problem of whether the representative elementary volume (REV obtained in the Darcy flow is also applicable to the case of the non-Darcy flow, the study on the REV size within the non-Darcy flow is proposed tentatively. The concept of the REV in the non-Darcy flow is based on the definition of the REV. According to the determination of the REV in the Darcy flow, the intrinsic permeability k and non-Darcy coefficient β are used simultaneously for the determination of the REV in the non-Darcy flow. The pore pressure cohesive element (PPCE is developed with the subroutine in ABAQUS. Then the simulation method of the Darcy and non-Darcy flow in the fractured rock mass is built using the PPCE. The proposed plan is examined through the comparison with existing research results. It is validated that this technic is efficient and accurate in simulating the Darcy and non-Darcy flow in the fractured rock mass. Combined with fracture networks generated by Monte Carlo Simulation technique, the PPCE is applied to the study on the REV size. Both conditions of the Darcy and non-Darcy flow are simulated for comparison. The simulation results of this model show that the REV of the non-Darcy flow is inconsistent with the REV of the Darcy flow, and the REV of the non-Darcy flow is more significant than the REV of the Darcy flow. The intrinsic permeability k tensors obtained in the Darcy flow and the non-Darcy flow are basically the same.

  2. The effects of cynodon dactylon on the immune response of NMRI-MICE after challenge with REV1

    Directory of Open Access Journals (Sweden)

    Behrooz Ilkhanizadeh

    2016-10-01

    Full Text Available Cynodon dactylon is used in Iranian traditional medicine as a healing agent for reducing the complications of diabetes mellitus. We proposed that Cynodon dactylon may perform its effects through moderating humoral and cellular immune responses. We aimed to determine the possible effects of hydroalcholic extract of Cynodon dactylonon humoral and cellular immune responses following the Rev1 challenge in the mouse model. 20 NMRI male mice were randomly grouped in two equal groups and immunized with Rev1[0.1 ml Rev1+0.9 PBS[. Mice in the treatment group orally received 400 mg/kg hydroalcoholic extract of Cynodon dactylon every day from the beginning of the study for 2 weeks. Blood samples were obtained from the animals 5 days after the last injection. Moreover, 48 hr before bleeding time, Rev1[0.1 ml Rev1+0.9 PBS[was injected into the left hind foot pad of mice. The levels of anti-Rev1 antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. Moreover, susceptibility of macrophages respiratory burst and proliferation of immune cells were measured in order withNitroblue tetrazolium[NBT] and Microculture Tetrazolium Assay [MTT]. The concentrations of IL-1, TNFα, Il-6, and IL-10 in the serum were determined using commercially available ELISA kits. We found a significant increase in anti-Rev1 antibody levels and simultaneously a significant decrease in the level of cellular immunity[DTH] in the treatment group compared to the control group. Lymphocyte proliferation index in splenocytes was significantly increased in the treatment group. However, the level of respiratory burst in phagocytic population of splenocytes dramatically decreased in the treatment group compared to the control. A significant decrease in IL-6, TNF-α , IL-1 and increaseIl-10 serum levels were also seen in the treatment group. Cynodon dactylon extract could have an anti-inflammatory effect through

  3. Infauna monitoring Horns Rev offshore wind farm. Annual status report 2003

    International Nuclear Information System (INIS)

    Bech, M.; Leonhars, S.B.; Pedersen, John

    2004-05-01

    ELSAM and ELTRA have established an offshore wind farm with an output of 160 MW in the waters of Horns Rev 1420 km off Blaevands Huk, which is the most westerly point of Denmark. The first phase of construction of the wind farm started in spring 2002. Before the construction activities took place, a baseline description of the benthos was conducted as a part of an environmental monitoring programme for the establishment of the Horns Rev Offshore Wind Farm. The baseline surveys for the present monitoring programme were conducted in the wind farm area on three occasions: spring 1999, spring 2001 and September 2001. In designated reference areas, surveys were conducted in spring 1999 and September 2001. The reference areas in 1999 and September 2001 were placed at two different geographical locations because the survey in September 2001 was planned to be a part of a fish monitoring programme. A comparison between the baseline study in spring 2001 and the baseline study in autumn 2001 clearly revealed that the biomass of most species increased considerably from spring to September. Despite the increase in biomass, the overall distribution of the species and their relative abundance did not change. In order to use the baseline data to investigate a possible impact after the construction of the wind farm, it was essential to arrange the monitoring programme either in spring or in September 2003, because the baseline studies were conducted in these periods. The monitoring programme was conducted in September 2003 after the wind farm had become operational, parallel with the survey on hard bottom substrates. The impacts of the wind farm on the benthic fauna (infauna) in the area were mainly expected to be due to the alteration of the local currents. As the changes in the currents are only minor, impacts on the water chemistry and on the benthic fauna resulting from hydrodynamic causes were expected to be limited or non-existent. The main objective of the present monitoring

  4. Infauna monitoring Horns Rev offshore wind farm. Annual state report 2004

    Energy Technology Data Exchange (ETDEWEB)

    Bech, M.; Frederiksen, R.; Pedersen, John; Leonhard, S.B.

    2005-04-15

    A total of 40 species were identified from the surveys in the Horns Rev area in September 2004 while 42 species were identified in 2003 and 47 species in September 2001. The decline in the number of species occurred both inside the wind farm and reference areas, which indicates that the decline could be a combination of changes in sediment characteristics and natural variation rather than an effect from the establishment of the wind farm. More species were not associated with the hard substrate at the turbine sites in 2004 compared to 2003, while in 2001, more species were associated with fine-grained sand. The median sediment grain size increased from 2001 to 2003 to 2004, which suggests that the velocity of the current increased, but modelling calculations on current speed predicted a 2% reduction in the wind farm area and up to a 15% reduction very close to the scour protection. These results agreed with the grain sizes found at the stations 5, 25 and 100 metres from the scour protection. At most stations, the medium grain size was 5 metres lower from the scour protection compared with the station 100 metres from the scour protection, which indicates that the velocity of the current was lower close to the scour protection. No significant impact on the infauna in the wind farm area was detectable concerning distance-related effects. Though general reductions in the population size of some of the character species in the surveyed areas might be related to changes in the sediment structure, the infauna community at Horns Rev showed no obvious sign of stress response as a consequence of possible impact from construction and operating activities. New species were observed in 2003 and 2004 and some of these might be a result of sediment characteristics, less predation or natural variation. The recording of other species might be a result of the introduction of hard bottom habitants in the wind farm area. The density of the most abundant bivalves and bristle worms was

  5. Horns Rev offshore wind power farm. Environmental impact assessment on water quality

    International Nuclear Information System (INIS)

    Andersen, Per

    2000-05-01

    As part of an overall Environmental Impact Assessment (EIA) undertaken in connection with a planned 150 MW offshore wind farm at Horns Rev, an assessment was made of the effects the wind farm would have on the water quality in the area. This EIA study was drawn up in accordance with the guidelines laid down by the Danish Ministry of Environment and Energy in the publication 'Guidelines for the preparation of EIA studies for offshore wind farms'. Horns Rev is situated off Blaevands Huk, which is Denmark's most westerly point. It is a shallow reef with water depths between 2 and 9 metres and is primarily composed of sand, gravel and pebbles. Only local and minor changes are anticipated in connection with the currents, sediments and wave conditions during the production phase. These will occur in the immediate vicinity of the individual foundations. For these reasons, no changes are expected in the water quality. This also includes also the pelagic primary production and the occurrence of plankton in the area. Increased local copper contamination of phytoplankton and zooplankton may be expected during the production phase, as a result of the total annual discharge of 206 kg copper from the slip-rings in the wind turbines. The contamination will potentially result in a local reduction of the pelagic primary production and changes in the species composition of the plankton. The wind turbines will be sandblasted and painted once during their lifetime, as part of the routine maintenance. The sandblasting and painting will lead to a temporary spill of paint, paint waste and sand. The impacts on water quality and plankton production are unknown. It is recommended that factors such as the toxicity of the paint be investigated, and that spills and the impact of waste be reduced as much as possible. The water quality and the plankton in the wind farm area and along the cable line's passage to shore through the international protected area, will only be affected in a minor way

  6. Infauna monitoring Horns Rev offshore wind farm. Annual status report 2004

    International Nuclear Information System (INIS)

    Bech, M.; Frederiksen, R.; Pedersen, John; Leonhard, S.B.

    2005-04-01

    A total of 40 species were identified from the surveys in the Horns Rev area in September 2004 while 42 species were identified in 2003 and 47 species in September 2001. The decline in the number of species occurred both inside the wind farm and reference areas, which indicates that the decline could be a combination of changes in sediment characteristics and natural variation rather than an effect from the establishment of the wind farm. More species were not associated with the hard substrate at the turbine sites in 2004 compared to 2003, while in 2001, more species were associated with fine-grained sand. The median sediment grain size increased from 2001 to 2003 to 2004, which suggests that the velocity of the current increased, but modelling calculations on current speed predicted a 2% reduction in the wind farm area and up to a 15% reduction very close to the scour protection. These results agreed with the grain sizes found at the stations 5, 25 and 100 metres from the scour protection. At most stations, the medium grain size was 5 metres lower from the scour protection compared with the station 100 metres from the scour protection, which indicates that the velocity of the current was lower close to the scour protection. No significant impact on the infauna in the wind farm area was detectable concerning distance-related effects. Though general reductions in the population size of some of the character species in the surveyed areas might be related to changes in the sediment structure, the infauna community at Horns Rev showed no obvious sign of stress response as a consequence of possible impact from construction and operating activities. New species were observed in 2003 and 2004 and some of these might be a result of sediment characteristics, less predation or natural variation. The recording of other species might be a result of the introduction of hard bottom habitants in the wind farm area. The density of the most abundant bivalves and bristle worms was

  7. Infauna monitoring Horns Rev offshore wind farm. Annual status report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Bech, M.; Leonhars, S.B.; Pedersen, John

    2004-05-15

    ELSAM and ELTRA have established an offshore wind farm with an output of 160 MW in the waters of Horns Rev 1420 km off Blaevands Huk, which is the most westerly point of Denmark. The first phase of construction of the wind farm started in spring 2002. Before the construction activities took place, a baseline description of the benthos was conducted as a part of an environmental monitoring programme for the establishment of the Horns Rev Offshore Wind Farm. The baseline surveys for the present monitoring programme were conducted in the wind farm area on three occasions: spring 1999, spring 2001 and September 2001. In designated reference areas, surveys were conducted in spring 1999 and September 2001. The reference areas in 1999 and September 2001 were placed at two different geographical locations because the survey in September 2001 was planned to be a part of a fish monitoring programme. A comparison between the baseline study in spring 2001 and the baseline study in autumn 2001 clearly revealed that the biomass of most species increased considerably from spring to September. Despite the increase in biomass, the overall distribution of the species and their relative abundance did not change. In order to use the baseline data to investigate a possible impact after the construction of the wind farm, it was essential to arrange the monitoring programme either in spring or in September 2003, because the baseline studies were conducted in these periods. The monitoring programme was conducted in September 2003 after the wind farm had become operational, parallel with the survey on hard bottom substrates. The impacts of the wind farm on the benthic fauna (infauna) in the area were mainly expected to be due to the alteration of the local currents. As the changes in the currents are only minor, impacts on the water chemistry and on the benthic fauna resulting from hydrodynamic causes were expected to be limited or non-existent. The main objective of the present monitoring

  8. Bird numbers and distribution in the Horns Rev offshore wind farm area. Annual status report 2003

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    This report presents data from six aerial surveys of birds in the Horns Rev wind farm area in 2003. Including 16 surveys conducted before construction of the wind farm started and three during the construction phase, a total of 25 surveys have been performed in the area since August 1999. Up until August 2002 the study area was surveyed from 26 north-south oriented, parallel transect lines. After that time four short transects were added eastwards from the previously easternmost transect. From August 2002 slight adjustments to the transect lines in the wind farm area had to be made in order to avoid collision, as survey altitude was 76 m and wind turbines are 110 m to highest wing tip. The six surveys in 2003 were performed on 13 February, 16 March, 23 April, 5 September, 4 and 30 December. The operational phase of the wind farm commenced in 2002. Hence the six surveys from 2003 are all considered post-construction data sets. A preliminary evaluation of the potential impact of the wind turbines on bird distributions has been carried out by comparison of these data to those from the 16 pre-construction surveys. (au)

  9. Domain- and nucleotide-specific Rev response element regulation of feline immunodeficiency virus production

    Science.gov (United States)

    Na, Hong; Huisman, Willem; Ellestad, Kristofor K.; Phillips, Tom R.; Power, Christopher

    2010-01-01

    Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analyses were performed in both an infectious FIV molecular clone and a FIV CAT-RRE reporter system. These studies disclosed that the stems within SLA (SA1, 2, 3, 4, and 5) of the RRE were critical but SA6 was not essential for FIV replication and CAT expression. These studies also revealed that the secondary structure rather than an antisense protein (ASP) mediates virus expression and replication in vitro. In addition, a single synonymous mutation within the FIV-RRE, SA3/45, reduced viral reverse transcriptase activity and p24 expression after transfection but in addition also showed a marked reduction in viral expression and production following infection. PMID:20570310

  10. Impacts de la déficience maternelle modérée en fer sur le développement des fonctions auditives et le métabolisme des acides gras et eicosanoïdes de la progéniture

    OpenAIRE

    Jougleux, Jean-Luc

    2015-01-01

    Tableau d'honneur de la Faculté des études supérieures et postdorales, 2014-2015 Cette étude a pour objectif de déterminer les impacts de la l’anémie ferriprive (AF) modérée durant la gestation et la lactation, sur les fonctions neurophysiologiques des voies auditives, ainsi que sur les fonctions métaboliques de la progéniture du cochon d’Inde (Cavia porcellus). Elle s’articule selon 2 volets d’études distincts (protocoles 1 et 2), basés tant sur la composition en fer des diètes, que sur l...

  11. Evolução da vassoura-de-bruxa e avaliação da resistência em progênies de cupuaçuzeiro Evolution of the witch's broom disease and evaluation of resistance in cupuassu progenies

    Directory of Open Access Journals (Sweden)

    Rafael Moysés Alves

    2009-12-01

    Full Text Available Com o objetivo de conhecer a evolução da doença vassoura-de-bruxa, a taxa de segregação e estimar parâmetros genéticos, foi conduzido um experimento com 21 progênies de irmãos completos de cupuaçuzeiro, instalado em Belém, Pará. As progênies foram obtidas pelo cruzamento controlado de clones resistentes com clones resistentes, clones resistentes com clones suscetíveis e, clones suscetíveis com clones suscetíveis. Foram avaliadas também três progênies de meios-irmãos como testemunhas. Os experimentos foram avaliados ao nível de indivíduos, e as análises foram conduzidas via metodologia de modelos lineares mistos (procedimento REMl/BlUP, como delineamento em blocos incompletos desbalanceados, com tratamentos comuns entre dois experimentos. As variáveis avaliadas foram: percentagem de plantas resistentes ao ataque da doença no ramo, inflorescência, fruto imaturo e maduro, no período de 2002 a 2007. Os resultados mostraram que a emissão de vassoura vegetativa é especialmente importante entre julho e setembro. A evolução da doença evidencia que as podas fitossanitárias devem ser realizadas no final da safra, nos meses de maio/junho, e repassadas em setembro/outubro. A população estudada oferece excelente possibilidade de seleção e ganho genético, respaldada pelos elevados índices de variabilidade genética e herdabilidade dos caracteres de resistência. As progênies segregaram tanto para os sintomas de vassoura nos ramos, nas inflorescências, como nos frutos imaturos e maduros. Para o controle integrado desta doença, os resultados mostram a importância da associação de materiais genéticos resistentes ou moderadamente resistentes à vassoura-de-bruxa com as podas fitossanitárias.This paper aimed to study the evolution and individual segregation of the witch's broom disease and estimate the genetic parameters in trials with 21 full sib families of Cupuaçú established in Belém, Pará. The progenies were

  12. Variação, divergência e correlações genéticas entre caracteres silviculturais e densidade básica da madeira em progênies de Myracrodruon urundeuva (Engler Fr. Allem. Genetic variation, divergence and correlations among silvicultural traits and wood density in progenies of Myracrodruon urundeuva (Engler Fr. Allem.

    Directory of Open Access Journals (Sweden)

    Eduardo Luiz LONGUI

    2011-06-01

    Full Text Available Myracrodruon urundeuva foi muito explorada no passado devido às qualidades de sua madeira e tornou-se escassa em todas as áreas naturais de ocorrência, sendo atualmente considerada como ameaçada de extinção (categoria vulnerável. Isso torna a espécie importante para estudos relacionados à estrutura genética de populações para fins de conservação e melhoramento genético. Assim, a partir de um teste de progênies, instalado em 1987, em Selvíria–MS, foram estimados parâmetros genéticos para a densidade básica da madeira em três posições radiais (medula, intermediária e casca, a distância genética de Mahalanobis e correlações genéticas e fenotípicas entre caracteres de crescimento (altura total, altura do fuste e diâmetro à altura do peito – DAP e densidade básica da madeira. A população apresentou alta densidade básica da madeira (0,75 g.cm-3 e alta herdabilidade (0,64 para este carácter na posição intermediária e na medula, indicando forte controle genético e a possibilidade de melhoramento por seleção. Os resultados também mostraram que para essa população de M. urundeuva deve-se utilizar as progênies 1 e 14 para obtenção de híbridos mais heteróticos por apresentarem maior distância de Mahalanobis; as progênies 14 e 20 por outro lado possuem distâncias muito próxima constituindo pares de progênies semelhantes, o que é interessante para se buscar melhoramento por retrocruzamento. Tais informações são importantes para escolha de métodos adequados de conservação e melhoramento genético.In the past, Myracrodruon urundeuva was intensively exploited due the quality of its wood and became scarce in all natural areas of occurrence, being actually considered in extinction (vulnerable category. This makes the species important for studies of population genetic structure for conservation and breeding purposes. Thus, from a progeny test established in 1987 in Selvíria, Mato Grosso do Sul

  13. Produção de Madeira de Progênies de Segunda Geração de Grevílea na Região de Avaré, SP Second Generation Half-sib Progenies Wood Production of Grevillea in the Region of Avaré - SP

    Directory of Open Access Journals (Sweden)

    Emerson Gonçalves Martins

    2011-03-01

    Full Text Available

    A grevílea (Grevillea robusta A. Cunn é uma espécie utilizada para o sombreamento de cafezais e de outras culturas agrícolas, áreas de pastagens e apicultura, paisagismo e como cortina quebra- ventos. Sua madeira também é utilizada para desdobro, em serrarias. Um teste com 28 progênies de meios-irmãos de segunda geração foi instalado no Município de Avaré, São Paulo. O objetivo principal foi avaliar e selecionar o material genético mais produtivo em volume de madeira. Dados de altura e diâmetro à altura do peito (DAP foram coletados na área experimental, na idade de três anos. Baseando-se nos dados estimados de volume cilíndrico com casca, as seguintes conclusões foram obtidas: em Avaré, SP, a progênie de segunda geração mais produtiva e com maior taxa de sobrevivência pertencente à procedência australiana de Boyd River (NSW. A progênie de segunda geração mais produtiva, em termos de volume cilíndrico de madeira com casca, foi 138 % superior à media geral do teste. Considerando o valor médio de volume de madeira para apenas as doze progênies de segunda geração mais produtivas, foi possível observar um aumento de 32 % em relação à média geral do experimento. 
    The Grevillea robusta A. Cunn is utilized for coffee plantation shading, crops growing and pasture shading, apiculture, ornamentation and wind breaks. It is also used to produce sawn wood. One test of 28 second generation half-sib progenies was planted in Forestry Institute of São Paulo, with 20°03’S latitude and 48°54’W logitude geographic coordenates and 630m of altitude. The main objectives of this paper were to select genetic material capable to produce more volume of wood. The total heigth and diameter at breast height (DBH were measured on the three yars old second generation half-sib progenies test. The volume was calculated and the results led to the following conclusion: The best

  14. Resposta imunitária à vacinação conjuntival com a estirpe Rev.1 de Brucella melitensis em ovinos e caprinos Serological response of sheep and goats to conjunctival Brucella melitensis Rev.1 vaccine

    Directory of Open Access Journals (Sweden)

    P. Poeta

    2003-04-01

    Full Text Available The live B. melitensis Rev.1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants, especially when used at the standard dose by the conjunctival route. In the present study a 1´ 10(9 CFU dose for both sheep and goats conjunctivally vaccinated was tested to evaluate the duration of serological responses. Conjunctival vaccination with Rev. 1 performed in adult animals induced a rapid rise in serological titres as measured by Rose Bengal Plate Test (RBPT, Complement Fixation Test (CF and Modified Rose Bengal Plate Test (MRBPT. Titres then decreased and became negative in most animals by four months after vaccination (except MRBPT. The goats responded better to the vaccination than the sheep as one month after vaccination 100% of the goats revealed positive results to RB and RBM and 93.4% to FC test. The RBM was the one that detected more positive animals along the study.

  15. Administrative circulars No. 22A (rev. 1) – Award of additional periods of membership in the Pensions Fund for long-term shift work and No. 22B (rev. 1) – Compensation for long-term shift work hours

    CERN Multimedia

    HR Department

    2011-01-01

    Administrative Circulars No. 22A (Rev. 1) entitled "Award of additional periods of membership in the Pension Fund for long-term shift work" and No. 22B (Rev.1) entitled “Compensation for long-term shift work hours”, adopted following discussion in the Standing Concertation Committee meeting of 21 September 2010 and entering into force on 1 March 2011, are available on the intranet site of the Human Resources Department: http://cern.ch/hr-docs/admincirc/admincirc.asp They cancel and replace Administrative Circulars No. 22A and 22B entitled "Award of additional periods of membership in the Pension Fund to shift workers (Early Departure)” and “Duration and special compensation for shift work” of January 2000. This new version clarifies, in particular, the compensation of effective long-term shift work hours. Department Head Office  

  16. HRensembleHR. High resolution ensemble for Horns Rev. Final project report; Offshore wind power

    Energy Technology Data Exchange (ETDEWEB)

    2010-03-15

    The wind farm output of an offshore-farm such as Horns Rev changes between nearly constant output to highly variable power output. A balance responsible will therefore benefit from knowing the variability of a wind farm in advance. Some understanding of the observed variability and the corresponding forecast error on offshore wind farms had been gathered in the past few years, while a large fraction (about 60%) of the error still lacked understanding and required further intense research. This was the outset at the beginning of the HREnsemble project. Results from the wave study, variability study, ocean coupling, findings from the sensitivity experiments, the iEnKF short-term forecast and finally the demonstration phase have given significant synergy. The most basic research result in the project is that the two empirical mode decomposition approaches, Hilbert-Huang and later the Ensemble Empirical Mode Decomposition (EEMD). Both approaches confirmed that significant variability exists in offshore conditions. It was found that the variability of the short time-scales (24 minutes) are either not explicitly visible in the grid-scale of the NWP models or in the best case significantly smoothed out in all of the tested model configurations. If we interpret 2/3 of the variability due to vertical waves not present in the mean flow and 1/3 of the variability due to meso-scale weather, then the model results and EEMD are consistent. We can however not verify this theory. At present we do not know if EEMD counts neither the events correct nor whether the ensemble forecast suppresses variability. The existence of variability above the time-scale related to friction between ocean waves and air can in fact explain some of the inconsistent results published in the literature and set a question mark behind the correctness of the calculation of friction in wave, ocean and weather modelling. (LN)

  17. NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1.

    Science.gov (United States)

    Güttler, Thomas; Madl, Tobias; Neumann, Piotr; Deichsel, Danilo; Corsini, Lorenzo; Monecke, Thomas; Ficner, Ralf; Sattler, Michael; Görlich, Dirk

    2010-11-01

    Classic nuclear export signals (NESs) confer CRM1-dependent nuclear export. Here we present crystal structures of the RanGTP-CRM1 complex alone and bound to the prototypic PKI or HIV-1 Rev NESs. These NESs differ markedly in the spacing of their key hydrophobic (Φ) residues, yet CRM1 recognizes them with the same rigid set of five Φ pockets. The different Φ spacings are compensated for by different conformations of the bound NESs: in the case of PKI, an α-helical conformation, and in the case of Rev, an extended conformation with a critical proline docking into a Φ pocket. NMR analyses of CRM1-bound and CRM1-free PKI NES suggest that CRM1 selects NES conformers that pre-exist in solution. Our data lead to a new structure-based NES consensus, and explain why NESs differ in their affinities for CRM1 and why supraphysiological NESs bind the exportin so tightly.

  18. ADMINISTRATIVE CIRCULAR No. 25 (REV. 3) - Special provisions for the fire and rescue service governing working and rest time

    CERN Multimedia

    2012-01-01

    Administrative Circular No. 25 (Rev. 3) entitled “Special provisions for the Fire and Rescue Service governing working and rest time”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 28 September 2012 and entering into force in October 2012, is available on the intranet site of the Human Resources Department: http://cern.ch/hr-docs/admincirc/admincirc.asp This Circular is applicable to staff members of the Fire and Rescue Service It cancels and replaces Administrative Circular No. 25 (Rev. 2) entitled “Shift work – Special provisions for the Fire and Rescue Service” of April 2003. This new version takes into account the new organisation of the Fire and Rescue Service, members of which will henceforth not exclusively perform their functions in the context of shift work, but also during reference working hours and during stand-by duty. Additionally, applicable limits regarding working and rest times an...

  19. Operational Circular No.2 (Rev. 2) - Conditions of access to the fenced parts of the CERN site

    CERN Multimedia

    2014-01-01

    Operational Circular No. 2 (Rev. 2) entitled “Conditions of access to the fenced parts of the CERN site” and its “implementation measures”, approved by the Director-General following discussion at the Standing Concertation Committee meeting on 20 May 2014 and entering into force on 1 September 2014, are available on the intranet site of the Human Resources Department (see here).   This circular is applicable to members of the personnel and other persons concerned. It cancels and replaces Operational Circular No. 2 (Rev. 1) entitled “Conditions of access to the fenced parts of the CERN site”, of April 1998. In particular, the revised circular provides for the possibility of mandating a person responsible for the proper implementation of the circular, specifies the rules relating to vehicles allowed on the site and the respective responsibilities of their owners, and relaxes certain administrative formalities in case of loss, theft or di...

  20. Operational Circular No. 2 (Rev. 3) - Conditions of access to the fenced parts of the CERN site

    CERN Multimedia

    HR Department

    2015-01-01

    Operational Circular No. 2 (Rev. 3) entitled "Conditions of access to the fenced parts of the CERN site", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 24 September 2015, is now available via this link.   This revised circular cancels and replaces Operational Circular No. 2 (Rev. 2) also entitled "Conditions of access to the fenced parts of the CERN site", of September 2014. The circular was revised predominantly in order to specify that access to the CERN site is granted to CERN Pension Fund beneficiaries only provided that they are actually in receipt of payments from the Fund; and to allow the Director-General to permit special types of vehicles on site, such as trailers. It also includes a certain number of text improvements and an updated version of the implementation measures, in particular with regard to vehicle identification, road traffic and parking.  

  1. Administrative circular n°3 (Rev. 2) – Home leave, travel to home station and assimilated leave and travel

    CERN Multimedia

    2013-01-01

    Administrative Circular No. 3 (Rev. 2) entitled “Home leave, travel to the home station and assimilated leave and travel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 11 October 2012 and entering into force on 1 January 2013, is available on the intranet site of the Human Resources Department.   This circular is applicable to employed members of the personnel. It cancels and replaces Administrative Circular No. 3 (Rev. 1) entitled “Travel to the home station and home leave” of June 2002. The circular was revised in order to take into account the new status of Associate Member State and the fact that henceforth, home stations may be situated on territory outside of Europe. It is proposed to introduce a new system of determination of the benefits (Travel expenses, travel time and distance indemnity) granted in the context of home leave and supplementary journeys to the home station.  For t...

  2. HRensembleHR. High resolution ensemble for Horns Rev. Final project report. Executive summary; Offshore wind power

    Energy Technology Data Exchange (ETDEWEB)

    2010-03-15

    The development of offshore wind power results in more energy production per area unit and new requirements to the generation forecasts. Measurements from Horns Rev and ensemble forecasts were used to upgrade forecasting tools for the relevant periods and time scales. The most significant development is a new algorithm for short-term forecasts that combines any relevant online measurements by means of ensemble forecasts. (ln)

  3. Comment on "Total Negative Refraction in Real Crystals for Ballistic Electrons and Light" (Phys. Rev. Lett. 91, 157404 (2003))

    OpenAIRE

    Yau, H. -F.; Liu, J. -P.; Ke, B.; Kuo, C. -H.; Ye, Z.

    2003-01-01

    Recently, Zhang et al. (Phys. Rev. Lett. 91, 157404 (2003)) have demonstrated that an amphoteric refraction, i. e. both positive and negative refraction, may prevail at the interface of two uniaxial anisotropic crystals when their optical axes are in different directions. The authors subsequently made a correspondence between such a refraction with the negative refraction expected for Left Handed Materials (LHMs). Here we comment that the amphoteric refraction can be observed even with one un...

  4. Evaluación externa en la LOMCE. Reválidas, exclusión y competitividad

    Directory of Open Access Journals (Sweden)

    Ángel I. Pérez Gómez

    2014-01-01

    Full Text Available La evaluación de las competencias o cualidades humanas que cada aprendiz ha de desarrollar de manera singular para afrontar la complejidad de la era digital contemporánea es incompatible con las reválidas, test y pruebas externas estandarizadas, iguales para todos. Las cualidades más importantes del aprendizaje en la era digital —autodirección, iniciativa, creatividad, pensamiento crítico, solución de problemas y autoevaluación— son difícilmente evaluables mediante instrumentos baratos y masivos «de papel y lápiz», como los test estandarizados o las reválidas externas; requieren, por el contrario, herramientas y procesos más complejos, plurales y flexibles como la observación continua de los equipos docentes sobre el quehacer y los procesos y productos de los aprendices (Soto Gómez, 2014. La LOMCE,1 fiel exponente de la pedagogía conservadora del movimiento GERM,2 recupera las reválidas, pues apuesta por la estandarización de aprendizajes superficiales en lugar de potenciar el desarrollo singular de cada aprendiz hasta el máximo de sus posibilidades.

  5. An analysis of international situation concerning nuclear security. Focused on the revision to INFCIRC/225/Rev.5

    International Nuclear Information System (INIS)

    Inamura, Tomoaki; Tanabe, Tomoyuki

    2011-01-01

    Since the September 11 attacks in 2001, counterterrorism measures have become not just domestic issues but critical issues that need international cooperation. Various nuclear security measures are in place as part of international counterterrorism measures. This report looks at the trend of international nuclear security measures to get implications for Japan, focusing on INFCIRC/225/Rev.5, an international guideline for physical protection of nuclear materials and nuclear facilities drawn up by IAEA. The observations of this report are as follows: 1) While legally binding nuclear security measures such as multilateral treaties and United Nations Security Council Resolutions impose minimum requirements on individual countries, the approaches led by IAEA or individual countries or private associations aim at more detailed consideration or information sharing to further improve nuclear security. 2) INFCIRC/225/Rev.5 comprises new concepts such as risk-based physical protection and nuclear security culture, as well as extended range of threats such as insiders or stand-off attacks and broader scope of measures to response. Japan should consider incorporation of INFCIRC/225/Rev.5 to its national laws and regulations putting in mind that it may have heavy influence and that Japan pledged to role leadership about nuclear security to international society. (author)

  6. Safety and efficacy of reduced doses of Brucella melitensis strain Rev. 1 vaccine in pregnant Iranian fat-tailed ewes

    Directory of Open Access Journals (Sweden)

    Mohammad Ebrahimi

    2012-12-01

    Full Text Available Brucellosis is one of the most important zoonotic diseases and is a significant cause of abortion in animals. Brucella melitensis strain Rev. 1 is recommended as the most effective vaccine for small ruminants but the application of full doses in adult animals is restricted. This study was conducted to determine a proper reduced dose of vaccine which confers protection but which is not abortifacient in Iranian fat-tailed sheep. A total of 51 non-vaccinated pregnant ewes were divided into three main groups and several subgroups. Ewes in different groups were vaccinated at different stages of pregnancy and various subgroups were subcutaneously immunised with different quantities of the micro-organism (7.5 × 106, 106, 5 × 105. Ewes again became pregnant a year later and were challenged with the wild-type strain to evaluate the protection conferred. Results revealed that the proportion of vaccination-induced abortions was significantly higher in ewes immunised with 7.5 × 106 Rev. 1 organisms than in those which received 106 or 5 × 105 bacteria. While 80% of non-vaccinated ewes aborted after challenge, none of the vaccinated ewes aborted post-challenge. This study indicated that a reduced dose of Rev. 1 vaccine containing 106 or 5 × 105 live cells could be safely used to induce protection in Iranian fat-tailed sheep at various stages of pregnancy.

  7. hREV3 is essential for error-prone translesion synthesis past UV or benzo[a]pyrene diol epoxide-induced DNA lesions in human fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Li Ziqiang; Zhang Hong; McManus, Terrence P.; McCormick, J. Justin; Lawrence, Christopher W.; Maher, Veronica M

    2002-12-29

    In S. cerevisiae, the REV3 gene, encoding the catalytic subunit of polymerase zeta, is involved in translesion synthesis and required for the production of mutations induced by ultraviolet radiation (UV) photoproducts and other DNA fork-blocking lesions, and for the majority of spontaneous mutations. To determine whether hREV3, the human homolog of yeast REV3, is similarly involved in error-prone translesion synthesis past UV photoproducts and other lesions that block DNA replication, an hREV3 antisense construct under the control of the TetP promoter was transfected into an infinite life span human fibroblast cell strain that expresses a high level of tTAk, the activator of that promoter. Three transfectant strains expressing high levels of hREV3 antisense RNA were identified and compared with their parental cell strain for sensitivity to the cytotoxic and mutagenic effects of UV. The three hREV3 antisense-expressing cell strains were not more sensitive than the parental strain to the cytotoxic effect of UV, but the frequency of mutants induced by UV in their HPRT gene was significantly reduced, i.e. to 14% that of the parent. Two of these hREV3 antisense-expressing cell strains were compared with the parental strain for sensitivity to ({+-})-7{beta},8{alpha}-dihydroxy-9{alpha},10{alpha}-epoxy-7,8,9,10-tetrahydro= benzo[a]pyrene (BPDE). They were not more sensitive than the parent strain to the cytotoxic effect of BPDE, but the frequency of mutants induced was significantly reduced, i.e. in one strain, to 17% that of the parent, and in the other, to 24%. DNA sequencing showed that the kinds of mutations induced by BPDE in the parental and the derivative strains did not differ and were similar to those found previously with finite life span human fibroblasts. The data strongly support the hypothesis that hRev3 plays a critical role in the induction of mutations by UV or BPDE. Because the level of hRev3 protein in human fibroblasts is below the level of antibody

  8. hREV3 is essential for error-prone translesion synthesis past UV or benzo[a]pyrene diol epoxide-induced DNA lesions in human fibroblasts

    International Nuclear Information System (INIS)

    Li Ziqiang; Zhang Hong; McManus, Terrence P.; McCormick, J. Justin; Lawrence, Christopher W.; Maher, Veronica M.

    2002-01-01

    In S. cerevisiae, the REV3 gene, encoding the catalytic subunit of polymerase zeta, is involved in translesion synthesis and required for the production of mutations induced by ultraviolet radiation (UV) photoproducts and other DNA fork-blocking lesions, and for the majority of spontaneous mutations. To determine whether hREV3, the human homolog of yeast REV3, is similarly involved in error-prone translesion synthesis past UV photoproducts and other lesions that block DNA replication, an hREV3 antisense construct under the control of the TetP promoter was transfected into an infinite life span human fibroblast cell strain that expresses a high level of tTAk, the activator of that promoter. Three transfectant strains expressing high levels of hREV3 antisense RNA were identified and compared with their parental cell strain for sensitivity to the cytotoxic and mutagenic effects of UV. The three hREV3 antisense-expressing cell strains were not more sensitive than the parental strain to the cytotoxic effect of UV, but the frequency of mutants induced by UV in their HPRT gene was significantly reduced, i.e. to 14% that of the parent. Two of these hREV3 antisense-expressing cell strains were compared with the parental strain for sensitivity to (±)-7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE). They were not more sensitive than the parent strain to the cytotoxic effect of BPDE, but the frequency of mutants induced was significantly reduced, i.e. in one strain, to 17% that of the parent, and in the other, to 24%. DNA sequencing showed that the kinds of mutations induced by BPDE in the parental and the derivative strains did not differ and were similar to those found previously with finite life span human fibroblasts. The data strongly support the hypothesis that hRev3 plays a critical role in the induction of mutations by UV or BPDE. Because the level of hRev3 protein in human fibroblasts is below the level of antibody detection, it was not

  9. Stimulation of nuclear receptor REV-ERBs regulates tumor necrosis factor-induced expression of proinflammatory molecules in C6 astroglial cells

    Energy Technology Data Exchange (ETDEWEB)

    Morioka, Norimitsu, E-mail: mnori@hiroshima-u.ac.jp; Tomori, Mizuki; Zhang, Fang Fang; Saeki, Munenori; Hisaoka-Nakashima, Kazue; Nakata, Yoshihiro

    2016-01-08

    Under physiological conditions, astrocytes maintain homeostasis in the CNS. Following inflammation and injury to the CNS, however, activated astrocytes produce neurotoxic molecules such as cytokines and chemokines, amplifying the initial molecular-cellular events evoked by inflammation and injury. Nuclear receptors REV-ERBα and REV-ERBβ (REV-ERBs) are crucial in the regulation of inflammation- and metabolism-related gene transcription. The current study sought to elucidate a role of REV-ERBs in rat C6 astroglial cells on the expression of inflammatory molecules following stimulation with the neuroinflammatory cytokine tumor necrosis factor (TNF). Stimulation of C6 cells with TNF (10 ng/ml) significantly increased the mRNA expression of CCL2, interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and matrix metalloprotease (MMP)-9, but not fibroblast growth factor-2 (FGF-2), cyclooxygenase-2 (COX-2) and MMP-2. Treatment with either REV-ERB agonists GSK4112 or SR9009 significantly blocked TNF-induced upregulation of CCL2 mRNA and MMP-9 mRNA, but not IL-6 mRNA and iNOS mRNA expression. Furthermore, treatment with RGFP966, a selective histone deacetylase 3 (HDAC3) inhibitor, potently reversed the inhibitory effects of GSK4112 on TNF-induced expression of MMP-9 mRNA, but not CCL2 mRNA. Expression of Rev-erbs mRNA in C6 astroglial cells, primary cultured rat cortical and spinal astrocytes was confirmed by reverse transcription polymerase chain reaction. Together, the findings demonstrate an anti-inflammatory effect, downregulating of MMP-9 and CCL2 transcription, of astroglial REV-ERBs activation through HDAC3-dependent and HDAC3-independent mechanisms. - Highlights: • Rev-erbα mRNA and Rev-erbβ mRNA are expressed in C6 astroglial cells. • TNF increases the expression of CCL2, IL-6, MMP-9 and iNOS mRNA. • REV-ERB activation inhibits CCL2 mRNA and MMP-9 mRNA expression. • HDAC3 activity is involved in the inhibitory effect of REV-ERB on MMP-9 induction.

  10. Stimulation of nuclear receptor REV-ERBs regulates tumor necrosis factor-induced expression of proinflammatory molecules in C6 astroglial cells

    International Nuclear Information System (INIS)

    Morioka, Norimitsu; Tomori, Mizuki; Zhang, Fang Fang; Saeki, Munenori; Hisaoka-Nakashima, Kazue; Nakata, Yoshihiro

    2016-01-01

    Under physiological conditions, astrocytes maintain homeostasis in the CNS. Following inflammation and injury to the CNS, however, activated astrocytes produce neurotoxic molecules such as cytokines and chemokines, amplifying the initial molecular-cellular events evoked by inflammation and injury. Nuclear receptors REV-ERBα and REV-ERBβ (REV-ERBs) are crucial in the regulation of inflammation- and metabolism-related gene transcription. The current study sought to elucidate a role of REV-ERBs in rat C6 astroglial cells on the expression of inflammatory molecules following stimulation with the neuroinflammatory cytokine tumor necrosis factor (TNF). Stimulation of C6 cells with TNF (10 ng/ml) significantly increased the mRNA expression of CCL2, interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and matrix metalloprotease (MMP)-9, but not fibroblast growth factor-2 (FGF-2), cyclooxygenase-2 (COX-2) and MMP-2. Treatment with either REV-ERB agonists GSK4112 or SR9009 significantly blocked TNF-induced upregulation of CCL2 mRNA and MMP-9 mRNA, but not IL-6 mRNA and iNOS mRNA expression. Furthermore, treatment with RGFP966, a selective histone deacetylase 3 (HDAC3) inhibitor, potently reversed the inhibitory effects of GSK4112 on TNF-induced expression of MMP-9 mRNA, but not CCL2 mRNA. Expression of Rev-erbs mRNA in C6 astroglial cells, primary cultured rat cortical and spinal astrocytes was confirmed by reverse transcription polymerase chain reaction. Together, the findings demonstrate an anti-inflammatory effect, downregulating of MMP-9 and CCL2 transcription, of astroglial REV-ERBs activation through HDAC3-dependent and HDAC3-independent mechanisms. - Highlights: • Rev-erbα mRNA and Rev-erbβ mRNA are expressed in C6 astroglial cells. • TNF increases the expression of CCL2, IL-6, MMP-9 and iNOS mRNA. • REV-ERB activation inhibits CCL2 mRNA and MMP-9 mRNA expression. • HDAC3 activity is involved in the inhibitory effect of REV-ERB on MMP-9 induction.

  11. Estimative of genetic parameters in progeny test of Pinus caribaea Morelet var. hondurensis Barret & Golfari by quantitative traits and microsatellite markers Estimativas de parâmetros genéticos em teste de progênies de Pinus caribaea var. hondurensis por caracteres quantitativos e marcadores microssatélites

    Directory of Open Access Journals (Sweden)

    Evandro Vagner Tambarussi

    2010-01-01

    Full Text Available The aims of this work were to estimate the coefficient of relatedness within families and the genetic parameters for growth related traits in a progeny test from an open-pollinated variety of Pinus caribaea Morelet hondurensis Barret & Golfari, established in Mato Grosso do Sul State, Brazil. The experimental design was the triple 10 x 10 lattice, with 96 families, three replicates, and ten plants per plot. Fourteen years after planting, the trial was measured for the following traits: total height, diameter at breast height (DBH, and true volume. The estimation of coefficients of relatedness within family from microsatellite loci indicated that families are true half-sibs (^r xy = 0.253. Thus, the additive genetic variance (σ2A can be estimated assuming that the genetic variance among progenies (σ2p accounts for ¼ of additive genetic variance (^σ2A = 4^σ2p. The estimative of heritability coefficients at individual level (h i² was relatively high (0.28 for DBH and 0.44 for height. The heritability coefficient considering the average families (h m² was also high, ranging among the traits from 0.50 to 0.58. These results suggest that the population can be improved by both massal and among families selection. Additionally, the estimated genetic gains with sequential selection among and within families were high (ranging from 8.92% for height to 37.56% for volume, demonstrating that this method of selection can generate high genetic improvement.Os objetivos deste trabalho foram estimar o coeficiente de parentesco dentro de famílias e os parâmetros genéticos e fenotípicos para os caracteres de crescimento (altura, diâmetro a altura do peito e volume em um teste de progênies de polinização aberta de Pinus caribaea Morelet var. hondurensis Barret & Golfari, implantado no Estado de Mato Grosso do Sul, Brasil. O teste foi implantado em látice 10 x 10 triplo, com 96 famílias, três repetições e dez plantas por parcela. Quatorze anos ap

  12. Tolerância ao florescimento precoce e características comerciais de progênies F4 de alface do cruzamento Regina 71 x Salinas 88 = Tolerance to precocious flowering and commerical traits of F4 progenies of hybrid Regina 71 x Salinas 88 lettuce

    Directory of Open Access Journals (Sweden)

    José Luiz Sandes de Carvalho Filho

    2009-01-01

    Full Text Available A alface é uma espécie cujas plantas possuem grande variabilidade no que diz respeito à forma, cor e textura das folhas, caracterizando diferentes tipos comerciais. O grupo folhas lisas ainda se mantém como um dos mais consumidos. A caracterização do tipo de folha é essencial no desenvolvimento de um programa de melhoramento,principalmente, quando a população segregante é oriunda de parentais contrastantes para o caráter. Além disso, a maior parte das cultivares utilizada é sensível ao calor, emitindo a haste floral precocemente. Assim, o objetivo deste trabalho foi avaliar características comercias de progênies F4 de alface e a sua tolerância ao calor, visando obter cultivares com folha lisa tolerantes ao florescimento precoce, resistentes aos nematóides e ao LMV. Avaliaram-se 22 progênies F4 originadas do cruzamento envolvendo as cultivares ‘Regina 71’ e ‘Salinas 88’, previamente selecionadas para tolerância ao calor, resistência aos nematóides das galhas e ao LMV (lettuce mosaic virus. O delineamento foi em blocos casualizados, com quatro repetições. Foram avaliados os tipos de limbo e borda foliares, formação de cabeça e número de dias para a primeira antese. Dentre as 22 progênies avaliadas, três mostraram-semais promissoras à obtenção de linhagens de alface de folhas lisas resistentes ao calor, nematóides das galhas e ao LMV.The lettuce is a plant that features great variability in form, color and texture. The butterleaf variety had remained one of themost consumed lettuce types. The evaluation of leaf type for commercial characters is essential for the development of any improvement program, especially when the parents are divergent types. Furthermore, most cultivars are susceptible to heat. Thus, the objective of the present work was to evaluate F4 progenies of butterleaf lettuce, aiming to select new genotypes for nematode resistance, tolerance to heat and LMV. Twenty-two F3:4 progenieswere

  13. Guidance and considerations for the implementation of INFCIRC/225/Rev.4, the physical protection of nuclear material and nuclear facilities

    International Nuclear Information System (INIS)

    2000-05-01

    This publication is intended to provide guidance and considerations for a State's competent authority to better understand and prescribe appropriate requirements, consistent with INFCIRC/225/Rev.4 for the protection of nuclear material and nuclear facilities which are compatible with accepted international practice. This report, together with a more detailed report, Handbook on the Physical Protection of Nuclear Material and Facilities, which addresses to the licencee or designer of physical protection systems who has specific implementation and compliance responsibilities, should be used in conjunction to each other to provide better and comprehensive guidance on physical protection

  14. Evolution of ASTEC V1.2 rev.1 code for WWER-1000 reactors/SBO sequence

    International Nuclear Information System (INIS)

    Georgieva, J.; Stefanova, A.; Groudev, P.; Tusheva, P.; Kalchev, B.; Passalacqua, R.

    2006-01-01

    In this paper a comparison between calculations of severe accidents occurred from WWER-1000 with ASTEC code specified for an event of full unloading with relief valves stuck opened with no hydroaccumulators intervention is presented. The purpose of the analyses provided is to present the relationship between the improvements of the actual version (ASTEC Vl.2 rev. 1) and ASTEC V1.1 p2 like: code modifications, incoming data improvements. Such discrepancies are to be examined. Case by case suggestions for ASTEC improvements are to be provided

  15. Elsam. Offshore Wind Farm. Horns Rev. Annual status report for the environmental monitoring programme 1. January 2001 - 31. December 2001

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-10-15

    As a result of the Danish Government's Energy Plan 21 a target of 5,500 MW wind power is to be erected in Denmark by 2030. 4,000 MW of these are to be placed offshore in special pointed areas with minimal impacts on the environment. In 1998 the Danish Ministry of Environment and Energy ordered two power companies, Elsam and Energi E2, to establish each a demonstration wind farm at one of the five pointed areas. The intention was to follow the environmental impacts from the wind farm and to evaluate the possibility of setting up about 1,500 MW in each area with as little impact on the environment as possible. In 1999 the two power companies were given approval to begin pre-studies of each of the two wind farms and the work on the site construction as well as the environmental impact assessment related hereto was initiated. The authorities made a number of requirements for the EIA surveys according to the EU-directive for preparation of EIA reports. In the summer of 2000 the EIA report with project description was submitted to the authorities and the project was approved in the spring of 2001. During the summer and autumn of 2001 orders were placed for the components for the wind farm, i.e. foundations, towers, wind turbines, cables etc. After having finalised the EIA, monitoring programmes of the wind farms were initiated on basis of the results of the surveys carried out during the EIA. This means that continuous surveys have been implemented for most of the environmental parameters from 1999 and till today. This annual status report for 2001 is to present the results from the annual environmental monitoring programme (the baseline studies) at Horns Rev, which form part of the monitoring programme set up for the Horns Rev project. To get a complete picture of the Danish monitoring programme for the national demonstration wind farm project it is necessary to see the report for Horns Rev and for Roedsand as a whole. The report comprises a description of the wind

  16. Elsam. Offshore Wind Farm. Horns Rev. Annual status report for the environmental monitoring programme 1. January 2001 - 31. December 2001

    International Nuclear Information System (INIS)

    2002-10-01

    As a result of the Danish Government's Energy Plan 21 a target of 5,500 MW wind power is to be erected in Denmark by 2030. 4,000 MW of these are to be placed offshore in special pointed areas with minimal impacts on the environment. In 1998 the Danish Ministry of Environment and Energy ordered two power companies, Elsam and Energi E2, to establish each a demonstration wind farm at one of the five pointed areas. The intention was to follow the environmental impacts from the wind farm and to evaluate the possibility of setting up about 1,500 MW in each area with as little impact on the environment as possible. In 1999 the two power companies were given approval to begin pre-studies of each of the two wind farms and the work on the site construction as well as the environmental impact assessment related hereto was initiated. The authorities made a number of requirements for the EIA surveys according to the EU-directive for preparation of EIA reports. In the summer of 2000 the EIA report with project description was submitted to the authorities and the project was approved in the spring of 2001. During the summer and autumn of 2001 orders were placed for the components for the wind farm, i.e. foundations, towers, wind turbines, cables etc. After having finalised the EIA, monitoring programmes of the wind farms were initiated on basis of the results of the surveys carried out during the EIA. This means that continuous surveys have been implemented for most of the environmental parameters from 1999 and till today. This annual status report for 2001 is to present the results from the annual environmental monitoring programme (the baseline studies) at Horns Rev, which form part of the monitoring programme set up for the Horns Rev project. To get a complete picture of the Danish monitoring programme for the national demonstration wind farm project it is necessary to see the report for Horns Rev and for Roedsand as a whole. The report comprises a description of the wind farm

  17. Guidance and considerations for the implementation of INFCIRC/225/Rev.4, the physical protection of nuclear material and nuclear facilities

    International Nuclear Information System (INIS)

    2002-05-01

    This publication is intended to provide guidance and considerations for a State's competent authority to better understand and prescribe appropriate requirements, consistent with INFCIRC/225/Rev.4 for the protection of nuclear material and nuclear facilities which are compatible with accepted international practice. This report, together with a more detailed report, Handbook on the Physical Protection of Nuclear Material and Facilities, which addresses to the licensee or designer of physical protection systems who has specific implementation and compliance responsibilities, should be used in conjunction to each other to provide better and comprehensive guidance on physical protection

  18. Binding mode prediction and MD/MMPBSA-based free energy ranking for agonists of REV-ERBα/NCoR.

    Science.gov (United States)

    Westermaier, Yvonne; Ruiz-Carmona, Sergio; Theret, Isabelle; Perron-Sierra, Françoise; Poissonnet, Guillaume; Dacquet, Catherine; Boutin, Jean A; Ducrot, Pierre; Barril, Xavier

    2017-08-01

    The knowledge of the free energy of binding of small molecules to a macromolecular target is crucial in drug design as is the ability to predict the functional consequences of binding. We highlight how a molecular dynamics (MD)-based approach can be used to predict the free energy of small molecules, and to provide priorities for the synthesis and the validation via in vitro tests. Here, we study the dynamics and energetics of the nuclear receptor REV-ERBα with its co-repressor NCoR and 35 novel agonists. Our in silico approach combines molecular docking, molecular dynamics (MD), solvent-accessible surface area (SASA) and molecular mechanics poisson boltzmann surface area (MMPBSA) calculations. While docking yielded initial hints on the binding modes, their stability was assessed by MD. The SASA calculations revealed that the presence of the ligand led to a higher exposure of hydrophobic REV-ERB residues for NCoR recruitment. MMPBSA was very successful in ranking ligands by potency in a retrospective and prospective manner. Particularly, the prospective MMPBSA ranking-based validations for four compounds, three predicted to be active and one weakly active, were confirmed experimentally.

  19. REMINDER: Compliance with Operational Circular No. 2 (Rev. 1) on “Conditions of access to the fenced CERN site”

    CERN Multimedia

    2012-01-01

    The purpose of Operational Circular No. 2 (Rev. 1) is to contribute to the protection of people and property by defining the conditions of access to the Organization's fenced sites. The behaviours that cannot be tolerated under any circumstances are: use of CERN access cards by people, other than the cardholders themselves, in order to gain access to facilities without having attended the required safety course; speeding, particularly on Route Gregory and Route Weisskopf; driving in and out of the site on the wrong side of the road; parking on spaces set aside for the disabled; nuisance parking, especially in the proximity of the restaurants; dumping of wrecked vehicles. As the aforementioned instances of non-compliance can lead to dangerous situations, the Organization reserves the right to apply the penalties provided for under paragraph 26 of Operational Circular No. 2 (Rev. 1), namely to refuse access to the site to people and/or their vehicles deemed to be in infringement of the circu...

  20. A numerical-statistical approach to determining the representative elementary volume (REV of cement paste for measuring diffusivity

    Directory of Open Access Journals (Sweden)

    Zhang, M. Z.

    2010-12-01

    Full Text Available Concrete diffusivity is a function of its microstructure on many scales, ranging from nanometres to millimetres. Multi-scale techniques are therefore needed to model this parameter. Representative elementary volume (REV, in conjunction with the homogenization principle, is one of the most common multi-scale approaches. This study aimed to establish a procedure for establishing the REV required to determine cement paste diffusivity based on a three-step, numerical-statistical approach. First, several series of 3D cement paste microstructures were generated with HYMOSTRUC3D, a cement hydration and microstructure model, for different volumes of cement paste and w/c ratios ranging from 0.30 to 0.60. Second, the finite element method was used to simulate the diffusion of tritiated water through these microstructures. Effective cement paste diffusivity values for different REVs were obtained by applying Fick’s law. Finally, statistical analysis was used to find the fluctuation in effective diffusivity with cement paste volume, from which the REV was then determined. The conclusion drawn was that the REV for measuring diffusivity in cement paste is 100x100x100 μm3.

    La difusividad del hormigón depende de su microestructura a numerosas escalas, desde nanómetros hasta milímetros, por lo que se precisa de técnicas multiescala para representar este parámetro. Junto con el principio de homogeneización, uno de los métodos multiescala más habituales es el volumen elemental representativo (VER. El objeto de este estudio era establecer un procedimiento que permitiera determinar el VER necesario para calcular la difusividad de la pasta de cemento, basándose en un método numéricoestadístico que consta de tres etapas. Primero, se crearon varias series de microestructuras de pasta de cemento en 3D con HYMOSTRUC3D, un programa que permite crear un modelo de la hidratación y microestructura del cemento. Luego se empleó el método de

  1. The HIV-1 Rev/RRE system is required for HIV-1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles

    Directory of Open Access Journals (Sweden)

    Ma Hong

    2011-06-01

    Full Text Available Abstract Background The process of HIV-1 genomic RNA (gRNA encapsidation is governed by a number of viral encoded components, most notably the Gag protein and gRNA cis elements in the canonical packaging signal (ψ. Also implicated in encapsidation are cis determinants in the R, U5, and PBS (primer binding site from the 5' untranslated region (UTR. Although conventionally associated with nuclear export of HIV-1 RNA, there is a burgeoning role for the Rev/RRE in the encapsidation process. Pleiotropic effects exhibited by these cis and trans viral components may confound the ability to examine their independent, and combined, impact on encapsidation of RNA into HIV-1 viral particles in their innate viral context. We systematically reconstructed the HIV-1 packaging system in the context of a heterologous murine leukemia virus (MLV vector RNA to elucidate a mechanism in which the Rev/RRE system is central to achieving efficient and specific encapsidation into HIV-1 viral particles. Results We show for the first time that the Rev/RRE system can augment RNA encapsidation independent of all cis elements from the 5' UTR (R, U5, PBS, and ψ. Incorporation of all the 5' UTR cis elements did not enhance RNA encapsidation in the absence of the Rev/RRE system. In fact, we demonstrate that the Rev/RRE system is required for specific and efficient encapsidation commonly associated with the canonical packaging signal. The mechanism of Rev/RRE-mediated encapsidation is not a general phenomenon, since the combination of the Rev/RRE system and 5' UTR cis elements did not enhance encapsidation into MLV-derived viral particles. Lastly, we show that heterologous MLV RNAs conform to transduction properties commonly associated with HIV-1 viral particles, including in vivo transduction of non-dividing cells (i.e. mouse neurons; however, the cDNA forms are episomes predominantly in the 1-LTR circle form. Conclusions Premised on encapsidation of a heterologous RNA into

  2. Hydroacoustic registration of fish abundance of offshore wind farms. Horns Rev offshore wind farm. Annual report 2004.

    Energy Technology Data Exchange (ETDEWEB)

    Hvidt, C.B.; Bruenner, L.; Reier Knudsen, F.

    2005-05-15

    Elsam Engineering AS has approved the implementation of a project concerning the registration of fish communities in Horns Rev Offshore Wind Farm with use of hydroacoustic methods. In a joint effort, Bio/consult as, Carl Bro as and SIMRAD AS have monitored the fish communities at Horns Rev Offshore Wind Farm using a new hydroacoustic methodology. The new hydroacoustic technique combines the use of scientific sonar acoustics with GPS to determine the density, diversity and location of fish. The objectives of this project were to test the possibility of using hydroacoustic techniques as alternative methods to traditional techniques to assess the fish assemblage inhabiting offshore wind farms and to investigate the possible effect from the wind farm and hard bottom substrates (turbine foundations) on fish abundance. The field study was carried out October 9-10, 2004 and consisted of four horizontal hydroacoustic survey transects each covering impact and reference areas. Transects were surveyed in order to achieve identical impact and reference transect pairs concerning environment, topography and time correspondence. The hydroacoustic equipment consisted of a SIMRAD EK60/EY60 echo sounder with a split-beam transducer (Simrad ES 120-4x10) mounted on a pan and tilt unit, a transceiver, a laptop extended with a GPS-receiver and additional large external hard discs. The raw data files from EK60 were converted to echogram files suitable for the post processing application, Sonar5-Pro. The Sonar5-Pro software makes it possible to filter out echo detections from the surface and the bottom, as well as perform cross filter detection. The validity of the results using the hydroacoustic method is high due to the cross filtering and single target tracking technique. From the hydroacoustic results, no or very little effect from the wind farm or from hard bottom substrates was found on the fish densities at Horns Rev Wind Farm at the time of the survey. The execution of the field

  3. LLNL Radiation Protection Program (RPP) Rev 9.2, Implementation of 10 CFR 835, 'Occupational Radiation Protection'

    Energy Technology Data Exchange (ETDEWEB)

    Shingleton, K. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2011-06-15

    The Department of Energy (DOE) originally issued Part 10 CFR 835, Occupational Radiation Protection, on January 1, 1994. This regulation, hereafter referred to as “the Rule”, required DOE contractors to develop and maintain a DOE-approved Radiation Protection Program (RPP); DOE approved the initial Lawrence Livermore National Laboratory (LLNL) RPP (Rev 2) on 6/29/95. DOE issued a revision to the Rule on December 4, 1998 and approved LLNL’s revised RPP (Rev 7.1) on 11/18/99. DOE issued a second Rule revision on June 8, 2007 (effective July 9, 2007) and on June 13, 2008 approved LLNL’s RPP (Rev 9.0) which contained plans and measures for coming into compliance with the 2007 Rule changes. DOE issued a correction to the Rule on April 21, 2009.

  4. Estimation of turbulence intensity using rotor effective wind speed in Lillgrund and Horns Rev-I offshore wind farms

    DEFF Research Database (Denmark)

    Gögmen, Tuhfe; Giebel, Gregor

    2016-01-01

    varies over the extent of the wind farm. This paper describes a method to estimate the TI at individual turbine locations by using the rotor effective wind speed calculated via high frequency turbine data. The method is applied to Lillgrund and Horns Rev-I offshore wind farms and the results are compared...... with TI derived from the meteorological mast, nacelle mounted anemometer on the turbines and estimation based on the standard deviation of power. The results show that the proposed TI estimation method is in the best agreement with the meteorological mast. Therefore, the rotor effective wind speed...... is shown to be applicable for the TI assessment in real-time wind farm calculations under different operational conditions. Furthermore, the TI in the wake is seen to follow the same trend with the estimated wake deficit which enables to quantify the turbulence in terms of the wake loss locally inside...

  5. Elsam. Offshore Wind Turbines. Horns Rev. Annual status report for the environmental monitoring programme 1. January 2004 - 31. December 2004

    International Nuclear Information System (INIS)

    2005-07-01

    In 2001, the former Ministry of the Environment and Energy granted Elsam A/S and Eltra A.m.b.A. approval to establish a wind farm capable of producing 160 MW of electric power, at Horns Rev, west of Blaevandshuk at the west coast of Jutland. According to the conditions of the approval, Elsam and Eltra were required to monitor the environmental effects of the wind farm by performing monitoring before, during and after construction of the wind farm. The environmental monitoring programme is financed through Public Service Obligation (PSO). Analogue to the Horns Rev wind farm another offshore demonstrational wind farm was established in the Baltic sea south of Lolland by Energi E2 the Nysted 165 MW offshore wind farm. To coordinate the environmental monitoring at the two demonstration wind farms at Nysted and Horns Rev within the framework of the approved budget, the Environmental Group of Danish Offshore Wind Farm Demonstration Projects was set up. The group consists of representatives from the Danish Forest and Nature Agency, the Danish Energy Authority, Elsam and Energi E2. The Environmental Group initiates, monitors and evaluates the environmental programmes continuously with respect to possible improvements and intensifications to ensure that the design of the individual programmes is optimal. After evaluation, the monitoring programmes are proposed by the Environmental Group and carried out after final approval by the Danish Energy Authority. The Environmental Group considers if there is reason to adjust the issues and priorities of the programmes based on previous experience or other inputs eg from the international expert panel, IAPEME (International Advisory Panel of Experts on Marine Ecology). IAPEME evaluates the environmental monitoring programmes and makes valuable suggestions and recommendations to the Environmental Group. The programmes have concentrated on the monitoring of possible impacts before, during and after construction to investigate and

  6. Amchitka Mud Pit Sites 2006 Post-Closure Monitoring and Inspection Report, Amchitka Island, Alaska, Rev. No.: 0

    Energy Technology Data Exchange (ETDEWEB)

    Matthews, Patrick

    2006-09-01

    In 2001, the U.S. Department of Energy (DOE), National Nuclear Security Administration (NNSA/NSO) remediated six areas associated with Amchitka mud pit release sites located on Amchitka Island, Alaska. This included the construction of seven closure caps. To ensure the integrity and effectiveness of remedial action, the mud pit sites are to be inspected every five years as part of DOE's long-term monitoring and surveillance program. In August of 2006, the closure caps were inspected in accordance with the ''Post-Closure Monitoring and Inspection Plan for Amchitka Island Mud Pit Release Sites'' (Rev. 0, November 2005). This post-closure monitoring report provides the 2006 cap inspection results.

  7. Elsam. Offshore Wind Turbines. Horns Rev. Annual status report for the environmental monitoring programme 1. January 2004 - 31. December 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-15

    In 2001, the former Ministry of the Environment and Energy granted Elsam A/S and Eltra A.m.b.A. approval to establish a wind farm capable of producing 160 MW of electric power, at Horns Rev, west of Blaevandshuk at the west coast of Jutland. According to the conditions of the approval, Elsam and Eltra were required to monitor the environmental effects of the wind farm by performing monitoring before, during and after construction of the wind farm. The environmental monitoring programme is financed through Public Service Obligation (PSO). Analogue to the Horns Rev wind farm another offshore demonstrational wind farm was established in the Baltic sea south of Lolland by Energi E2 the Nysted 165 MW offshore wind farm. To coordinate the environmental monitoring at the two demonstration wind farms at Nysted and Horns Rev within the framework of the approved budget, the Environmental Group of Danish Offshore Wind Farm Demonstration Projects was set up. The group consists of representatives from the Danish Forest and Nature Agency, the Danish Energy Authority, Elsam and Energi E2. The Environmental Group initiates, monitors and evaluates the environmental programmes continuously with respect to possible improvements and intensifications to ensure that the design of the individual programmes is optimal. After evaluation, the monitoring programmes are proposed by the Environmental Group and carried out after final approval by the Danish Energy Authority. The Environmental Group considers if there is reason to adjust the issues and priorities of the programmes based on previous experience or other inputs eg from the international expert panel, IAPEME (International Advisory Panel of Experts on Marine Ecology). IAPEME evaluates the environmental monitoring programmes and makes valuable suggestions and recommendations to the Environmental Group. The programmes have concentrated on the monitoring of possible impacts before, during and after construction to investigate and

  8. Visual and radar observations of birds in relation to collision risk at the Horns Rev offshore wind farm. Annual status report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Kjaer Christensen, T.; Hounisen, J.P.; Clausager, I.; Krag Petersen, I.

    2004-07-01

    The aim of the project is to assess the collision risk between birds and wind turbines at the Horns Rev wind farm. In 2003 the studies focused on describing bird movements in relation to the wind farm and to identify the species-specific behavioural responses towards the wind turbines shown by migrating and staging species. The Horns Rev area lies in a region known to be important for substantial water bird migration as well as holding internationally important numbers of several wintering and staging water bird species. (au)

  9. Avaliação genética de indivíduos e progênies de cupuaçuzeiro no estado do Pará e estimativas de parâmetros genéticos Genetic evaluation of individuals and progenies of Theobroma grandiflorum in the state of Pará and estimates of genetic parameters

    Directory of Open Access Journals (Sweden)

    Rafael Moysés Alves

    2008-09-01

    Full Text Available O presente trabalho relata a avaliação genotípica de progênies de cupuaçuzeiro, no Estado do Pará, para os caracteres número de frutos (NF em quatro safras, intensidade de ocorrência de vassoura- de-bruxa na inflorescência (VBI e nos frutos (VBF, e peso de vassoura-de-bruxa (PVB. Apresenta também estimativas de parâmetros genéticos que permitem inferir sobre o controle genético e nível de variabilidade genética presente no material avaliado. Todos os caracteres apresentaram considerável variabilidade genética, com coeficientes de variação genética variando de 27% a 88% no âmbito de progênie e de 38% a 123% no âmbito individual. Isto revela excelentes possibilidades para a seleção nessa população experimental híbrida. As estimativas de herdabilidade individual no sentido restrito, em uma safra, variaram de 25% a 54%, e as repetibilidades individuais para NF equivaleram a 35%. Com as quatro safras realizadas, a herdabilidade em nível individual aumentou para 48%, propiciando acurácia seletiva de 70%, para a seleção de indivíduos. O ganho em eficiência, quando se usa mais de cinco safras, é praticamente desprezível. Para NF, ganhos acima de 60% podem ser obtidos com a seleção dos cinco melhores indivíduos. Poderão ser selecionados indivíduos com produção anual de 17 frutos, valor muito superior à média geral de 10 frutos, encontrada nos plantios comerciais. Verificam-se ganhos genéticos bastante superiores quando se faz a propagação clonal dos melhores indivíduos em relação ao que se verifica quando se realiza a propagação sexuada. Para o melhor indivíduo, o ganho genético aumenta de 75.5% para 88.3%, ou seja, de 17 para quase 19 frutos por planta. Isto revela um grande potencial para a clonagem comercial de cupuaçuzeiro. Para os caracteres VBI e VBF, verificaram-se altas herdabilidades individuais no sentido restrito com valores variando entre 30% e 54%. Isto revela o excelente potencial da

  10. 78 FR 11907 - Notice of Forthcoming Workshop To Discuss Revisions to NUREG/BR-0204, Rev. 2 “Instructions for...

    Science.gov (United States)

    2013-02-20

    ... radioactive waste disposal facilities if over-estimated. Additionally, the NRC staff received comments from... Performance Assessment Methodology for Low-Level Radioactive Waste Disposal Facilities'' (ADAMS Accession No..., Rev. 2 ``Instructions for Completing NRC's Uniform Low-Level Radioactive Waste Manifest.'' Information...

  11. Post-construction evaluation of bird abundances and distributions in the Horns Rev 2 offshore wind farm area, 2011 and 2012

    DEFF Research Database (Denmark)

    Petersen, Ib Krag; Nielsen, Rasmus Due; Mackenzie, Monique L.

    2014-01-01

    Optællinger af fugle fra fly efter linjetransekt-metoden blev anvendt til at beskrive fuglenes antal og udbredelse i undersøgelsesområdet. Ti optællinger før Horns Rev 2 mølleparkens opførelse blev sammenlignet med ti optællinger foretaget efter parkens etablering. Rumlig modellering blev anvendt...

  12. Evaluation of vaccination strategies against infection with feline immunodeficiency virus (FIV) based on recombinant viral vectors expressing FIV Rev and OrfA

    NARCIS (Netherlands)

    Huisman, W.; Schrauwen, E.J.A.; Tijhaar, E.; Süzer, Y.; Pas, S.D.; Amerongen, van G.; Sutter, G.; Rimmelzwaan, G.F.; Osterhaus, A.D.M.E.

    2008-01-01

    In recent years it has become clear that cell-mediated immunity is playing a role in the control of lentivirus infections. In particular, cytotoxic T lymphocyte responses have been associated with improved outcome of infection, especially those directed against the regulatory proteins like Rev and

  13. Situation analysis of physical independence of the equipment and safety circuits of Almaraz NPP regarding R.G. 1.75 rev.3 (2005)

    International Nuclear Information System (INIS)

    Seijas Portela, S.

    2010-01-01

    Situation analysis of physical independence of the electrical equipment and circuits CN safety Almaraz about R.G. 1.75 rev. 3. (2005) The aim of this paper is to present the work done in the analysis of the physical separation of redundant safety electrical equipment (emergency diesel generators, medium voltage, electrical cabinets, etc.) and physical separation of circuits and electrical conduits.

  14. 75 FR 75486 - Maritime Security Directive 104-6 (Rev. 4); Guidelines for U.S. Vessels Operating in High Risk...

    Science.gov (United States)

    2010-12-03

    ... Directive 104-6 (Rev. 4); Guidelines for U.S. Vessels Operating in High Risk Waters AGENCY: Coast Guard, DHS... Maritime Transportation Security Act (MTSA) on international voyages through or in designated high risk... MARSEC Directives are available at your local Captain of the Port (COTP) office. Phone numbers and...

  15. 76 FR 2402 - Maritime Security Directive 104-6 (Rev 5); Guidelines for U.S. Vessels Operating in High Risk Waters

    Science.gov (United States)

    2011-01-13

    ... Directive 104-6 (Rev 5); Guidelines for U.S. Vessels Operating in High Risk Waters AGENCY: Coast Guard, DHS... designated high risk waters, and provides additional counter-piracy guidance and mandatory measures for these... MARSEC Directives are available at your local Captain of the Port (COTP) office. Phone numbers and...

  16. Investigations of migratory birds during operation of Horns Rev offshore wind farm: Preliminary note of analysis of data from spring 2004

    International Nuclear Information System (INIS)

    Kjaer Christensen, T.; Hounisen, J.P.

    2004-01-01

    In February 1998, the Ministry of the Environment gave Elsam A/S and Eltra A.m.b.a. approval to erect a wind farm, capable of producing 160 MW of electric power, at Horns Rev, west of Blaevandshuk off the west coast of Jutland. Construction activities at Horns Rev started in September 2001 and were finished in summer 2002. The entire project has been organised as a demonstration project to assess the technical, economic and environmental constraints on the future development of electric power production in Danish offshore environments. Within the framework of the environmental programme, bird investigations have been carried out in relation to the risk of collision between birds and wind turbines since 2002. To provide the latest update on the results from the bird investigations, this note presents results compiled during spring 2004 and deals with a preliminary analysis of effects on birds present at Horns Rev during commercial operation of the Horns Rev wind farm. Due to the remoteness of the area it has not been possible to obtain base-line investigation of bird occurrence and behaviour at the wind farm site. (au)

  17. Specificity of RSG-1.2 peptide binding to RRE-IIB RNA element of HIV-1 over Rev peptide is mainly enthalpic in origin.

    Science.gov (United States)

    Kumar, Santosh; Bose, Debojit; Suryawanshi, Hemant; Sabharwal, Harshana; Mapa, Koyeli; Maiti, Souvik

    2011-01-01

    Rev is an essential HIV-1 regulatory protein which binds to the Rev responsive element (RRE) present within the env gene of HIV-1 RNA genome. This binding facilitates the transport of the RNA to the cytoplasm, which in turn triggers the switch between viral latency and active viral replication. Essential components of this complex have been localized to a minimal arginine rich Rev peptide and stem IIB region of RRE. A synthetic peptide known as RSG-1.2 binds with high binding affinity and specificity to the RRE-IIB than the Rev peptide, however the thermodynamic basis of this specificity has not yet been addressed. The present study aims to probe the thermodynamic origin of this specificity of RSG-1.2 over Rev Peptide for RRE-IIB. The temperature dependent melting studies show that RSG-1.2 binding stabilizes the RRE structure significantly (ΔT(m) = 4.3°C), in contrast to Rev binding. Interestingly the thermodynamic signatures of the binding have also been found to be different for both the peptides. At pH 7.5, RSG-1.2 binds RRE-IIB with a K(a) = 16.2±0.6×10(7) M(-1) where enthalpic change ΔH = -13.9±0.1 kcal/mol is the main driving force with limited unfavorable contribution from entropic change TΔS = -2.8±0.1 kcal/mol. A large part of ΔH may be due to specific stacking between U72 and Arg15. In contrast binding of Rev (K(a) = 3.1±0.4×10(7) M(-1)) is driven mainly by entropy (ΔH = 0 kcal/mol and TΔS = 10.2±0.2 kcal/mol) which arises from major conformational changes in the RNA upon binding.

  18. Sociological investigation of the reception of Horns Rev and Nysted offshore wind farms in the local communities[Denmark

    Energy Technology Data Exchange (ETDEWEB)

    Kuehn, S.

    2005-03-15

    This annual report presents the work related to the sociological part of the socio-economic project on the local communities' reception of the Horns Rev Offshore Wind Farm, west of Blaavands Huk in Jutland, and the Nysted Offshore Wind Farm, south of Lolland. The project is part of the monitoring programme in connection with the construction of the offshore wind farms. Unlike projects, which examine the impact of the wind farms on nature, the socio-economic project did not start until 2003. The socio-economic project examines the effects on the local communities. Among other things the environmental-economic part of the project examines the preferences of the population in relation to location of the wind farm and the willingness to pay for increasing the distance between the wind farms and the coast. The sociological survey is qualitative. The survey is divided into three phases, as the aim is to investigate the public community's attitude towards the erection of an offshore wind farm before and after the construction of the wind farm. This aim is based on the assumption that attitudes may change once the wind farm is erected and the population has experienced the visibility of the wind farm, etc. The division into phases is as follows: 1) Phase 1 treats the conditions as they were before the erection of Nysted Offshore Wind Farm. 2) Phase 2 is an investigation of the development in attitudes towards Horns Rev Offshore Wind Farm a year after erection. 3) Phase 3 comprises a follow-up investigation of the local area at Nysted Offshore Wind Farm. This annual report treats phases 1 and 2. Phase 1 was carried out in April-August 2003, and the interviews at Nysted were thus made during the erection of the first offshore wind turbines. Phase 2 was carried out in September-November 2003. The follow-up investigation of the conditions in the local area around Nysted offshore wind farm is to be carried out in August-December 2004. The results of the investigations

  19. Expression of the clock gene Rev-erbα in the brain controls the circadian organisation of food intake and locomotor activity, but not daily variations of energy metabolism

    NARCIS (Netherlands)

    Sen, Satish; Dumont, Stéphanie; Sage-Ciocca, Dominique; Reibel, Sophie; de Goede, Paul; Kalsbeek, Andries; Challet, Etienne

    2018-01-01

    The nuclear receptor REV-ERBα is part of the molecular clock mechanism and is considered to be involved in a variety of biological processes within metabolically active peripheral tissues as well. To investigate whether Rev-erbα (also known as Nr1d1) in the brain plays a role in the daily variations

  20. Xpf and not the Fanconi anaemia proteins or Rev3 accounts for the extreme resistance to cisplatin in Dictyostelium discoideum.

    Directory of Open Access Journals (Sweden)

    Xiao-Yin Zhang

    2009-09-01

    Full Text Available Organisms like Dictyostelium discoideum, often referred to as DNA damage "extremophiles", can survive exposure to extremely high doses of radiation and DNA crosslinking agents. These agents form highly toxic DNA crosslinks that cause extensive DNA damage. However, little is known about how Dictyostelium and the other "extremophiles" can tolerate and repair such large numbers of DNA crosslinks. Here we describe a comprehensive genetic analysis of crosslink repair in Dictyostelium discoideum. We analyse three gene groups that are crucial for a replication-coupled repair process that removes DNA crosslinks in higher eukarya: The Fanconi anaemia pathway (FA, translesion synthesis (TLS, and nucleotide excision repair. Gene disruption studies unexpectedly reveal that the FA genes and the TLS enzyme Rev3 play minor roles in tolerance to crosslinks in Dictyostelium. However, disruption of the Xpf nuclease subcomponent results in striking hypersensitivity to crosslinks. Genetic interaction studies reveal that although Xpf functions with FA and TLS gene products, most Xpf mediated repair is independent of these two gene groups. These results suggest that Dictyostelium utilises a distinct Xpf nuclease-mediated repair process to remove crosslinked DNA. Other DNA damage-resistant organisms and chemoresistant cancer cells might adopt a similar strategy to develop resistance to DNA crosslinking agents.

  1. Comment on “Diffusion of n-type dopants in germanium” [Appl. Phys. Rev. 1, 011301 (2014)

    International Nuclear Information System (INIS)

    Cowern, N. E. B.; Simdyankin, S.; Goss, J. P.; Napolitani, E.; De Salvador, D.; Bruno, E.; Mirabella, S.; Ahn, C.; Bennett, N. S.

    2015-01-01

    The authors of the above paper call into question recent evidence on the properties of self-interstitials, I, in Ge [Cowern et al., Phys. Rev. Lett. 110, 155501 (2013)]. We show that this judgment stems from invalid model assumptions during analysis of data on B marker-layer diffusion during proton irradiation, and that a corrected analysis fully supports the reported evidence. As previously stated, I-mediated self-diffusion in Ge exhibits two distinct regimes of temperature, T: high-T, dominated by amorphous-like mono-interstitial clusters—i-morphs—with self-diffusion entropy ≈30 k, and low-T, where transport is dominated by simple self-interstitials. In a transitional range centered on 475 °C both mechanisms contribute. The experimental I migration energy of 1.84 ± 0.26 eV reported by the Münster group based on measurements of self-diffusion during irradiation at 550 °C < T < 680 °C further establishes our proposed i-morph mechanism

  2. Horns Rev offshore wind farm. Introducing hard bottom substrate sea bottom and marine biology. Data report 2001

    International Nuclear Information System (INIS)

    Leonhard, S.B.; Pedersen, John

    2002-08-01

    The Ministry of Environment and Energy requested ELSAM and ELTRA to establish an offshore wind farm with an output of 150 MW in the waters of Horns Rev, approximately 15 km off Blaevandshuk, which is the most westerly point of Denmark. The first phase of construction of the wind farm have started in spring 2002. Before the construction activities take place, a baseline description of the benthos has been conducted as a part of an environmental monitoring programme for the introduction of hard bottom substrates in the North Sea. The establishment of a monitoring programme is required according to some environmental guidelines for offshore wind farms prepared by the Danish Energy Agency. The monitoring programme established for the benthic infauna was performed in spring 2001. In addition to a proposed fish investigation programme concerning the stomach contents of fish a comparative programme on benthos was established as part of the monitoring programme. The benthos sampling in connection with the fish programme was conducted in autumn 2001. This benthic survey includes sampling in a proposed reference area for the fish surveys north east of the wind farm. This report presents the data of the baseline environmental survey of the seabed in the wind farm site and in the proposed reference site and a brief description of the weather conditions at the time of sampling. (au)

  3. Numerical modelling of the flow in the resin infusion process on the REV scale: A feasibility study

    Energy Technology Data Exchange (ETDEWEB)

    Jabbari, M.; Spangenberg, J.; Hattel, J. H. [Process Modelling Group, Department of Mechanical Engineering, Technical University of Denmark, Nils Koppels Allé, 2800 Kgs. Lyngby (Denmark); Jambhekar, V. A.; Helmig, R. [Department of Hydromechanics and Modelling of Hydrosystems, Institute for Modelling Hydraulic and Environmental Systems, Universität Stuttgart, Stuttgart (Germany); Gersborg, A. R. [SCION DTU, Diplomvej 373N, DK-2800 Lyngby (Denmark)

    2016-06-08

    The resin infusion process (RIP) has developed as a low cost method for manufacturing large fibre reinforced plastic parts. However, the process still presents some challenges to industry with regards to reliability and repeatability, resulting in expensive and inefficient trial and error development. In this paper, we show the implementation of 2D numerical models for the RIP using the open source simulator DuMu{sup X}. The idea of this study is to present a model which accounts for the interfacial forces coming from the capillary pressure on the so-called representative elementary volume (REV) scale. The model is described in detail and three different test cases — a constant and a tensorial permeability as well as a preform/Balsa domain — are investigated. The results show that the developed model is very applicable for the RIP for manufacturing of composite parts. The idea behind this study is to test the developed model for later use in a real application, in which the preform medium has numerous layers with different material properties.

  4. A comprehensive experiment for molecular biology: Determination of single nucleotide polymorphism in human REV3 gene using PCR-RFLP.

    Science.gov (United States)

    Zhang, Xu; Shao, Meng; Gao, Lu; Zhao, Yuanyuan; Sun, Zixuan; Zhou, Liping; Yan, Yongmin; Shao, Qixiang; Xu, Wenrong; Qian, Hui

    2017-07-08

    Laboratory exercise is helpful for medical students to understand the basic principles of molecular biology and to learn about the practical applications of molecular biology. We have designed a lab course on molecular biology about the determination of single nucleotide polymorphism (SNP) in human REV3 gene, the product of which is a subunit of DNA polymerase ζ and SNPs in this gene are associated with altered susceptibility to cancer. This newly designed experiment is composed of three parts, including genomic DNA extraction, gene amplification by PCR, and genotyping by RFLP. By combining these activities, the students are not only able to learn a series of biotechniques in molecular biology, but also acquire the ability to link the learned knowledge with practical applications. This comprehensive experiment will help the medical students improve the conceptual understanding of SNP and the technical understanding of SNP detection. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(4):299-304, 2017. © 2017 The International Union of Biochemistry and Molecular Biology.

  5. Base-line investigations of birds in relation to an offshore wind farm at Horns Rev: results and conclusions 2000/2001

    Energy Technology Data Exchange (ETDEWEB)

    Kjaer Christensen, T.; Clausager, I.; Krag Petersen, I. [NERI, Dept. of Coastal Zone Ecology, Roskilde (Denmark)

    2001-07-01

    This report presents the combined results of two years of base-line investigations of birds performed during August 1999 - April 2001 in relation to the proposed construction of an offshore wind farm at Horns Rev in the Danish part of the North Sea ca 14 km southwest of Blaevandshuk. Based on the distribution of the most abundant bird species recorded during 13 aerial surveys, there were no indications that the wind farm area was of any particular importance to the birds' exploitation of the Horns Rev area. Fish-eating species like divers, gannet, terns, auks and gulls generally showed scattered and variable distributions, occurring in the areas north and south of Horns Rev, and with low numbers occurring on the reef proper and within the planned wind farm area. The distribution of benthic foraging species, eider and common scoter, showed that they mainly exploited the coastal parts of the area off Blaevandshuk and Skallingen, although the common scoter was found in relatively high numbers on the southeast slopes of Horns Rev and within the wind farm area in the April 2001 survey. Preference analyses of bird exploitation of the Horns Rev area showed that if the birds completely avoid the wind farm area after erection of the wind turbines, this will affects less than 1% of the different species, except divers of which 1.65% will be affected. If the birds avoid the wind farm area and an adjacent 4 km zone (worst case scenario), it is estimated to affect 11% of the common scoter, 10% of the gannet, 7-9% of the divers, alcids and velvet scoter and 0-6% of the remaining species. The seasonal occurrence of the recorded species was fully comparable to the seasonal occurrence of these species recorded at Blaevandshuk since 1963. Year-to-year variation in abundance between the seasons August 1999 - April 2000 and August 2000 - April 2001 was mainly found in species that migrate through the Horns Rev area (terns, gannet, kittiwake), and with less pronounced variation in

  6. Base-line investigations of birds in relation to an offshore wind farm at Horns Rev: results and conclusions 2000/2001

    International Nuclear Information System (INIS)

    Kjaer Christensen, T.; Clausager, I.; Krag Petersen, I.

    2001-01-01

    This report presents the combined results of two years of base-line investigations of birds performed during August 1999 - April 2001 in relation to the proposed construction of an offshore wind farm at Horns Rev in the Danish part of the North Sea ca 14 km southwest of Blaevandshuk. Based on the distribution of the most abundant bird species recorded during 13 aerial surveys, there were no indications that the wind farm area was of any particular importance to the birds' exploitation of the Horns Rev area. Fish-eating species like divers, gannet, terns, auks and gulls generally showed scattered and variable distributions, occurring in the areas north and south of Horns Rev, and with low numbers occurring on the reef proper and within the planned wind farm area. The distribution of benthic foraging species, eider and common scoter, showed that they mainly exploited the coastal parts of the area off Blaevandshuk and Skallingen, although the common scoter was found in relatively high numbers on the southeast slopes of Horns Rev and within the wind farm area in the April 2001 survey. Preference analyses of bird exploitation of the Horns Rev area showed that if the birds completely avoid the wind farm area after erection of the wind turbines, this will affects less than 1% of the different species, except divers of which 1.65% will be affected. If the birds avoid the wind farm area and an adjacent 4 km zone (worst case scenario), it is estimated to affect 11% of the common scoter, 10% of the gannet, 7-9% of the divers, alcids and velvet scoter and 0-6% of the remaining species. The seasonal occurrence of the recorded species was fully comparable to the seasonal occurrence of these species recorded at Blaevandshuk since 1963. Year-to-year variation in abundance between the seasons August 1999 - April 2000 and August 2000 - April 2001 was mainly found in species that migrate through the Horns Rev area (terns, gannet, kittiwake), and with less pronounced variation in

  7. RevSex duplication-induced and sex-related differences in the SOX9 regulatory region chromatin landscape in human fibroblasts.

    Science.gov (United States)

    Lybæk, Helle; de Bruijn, Diederik; den Engelsman-van Dijk, Anke H A; Vanichkina, Darya; Nepal, Chirag; Brendehaug, Atle; Houge, Gunnar

    2014-03-01

    It was recently shown that duplications of the RevSex element, located 0.5 Mb upstream of SOX9, cause XX-disorder of sex development (DSD), and that deletions cause XY-DSD. To explore how a 148 kb RevSex duplication could have turned on gonadal SOX9 expression in the absence of SRY in an XX-male, we examined the chromatin landscape in primary skin fibroblast cultures from the index, his RevSex duplication-carrier father and six controls. The ENCODE project supports the notion that chromatin state maps show overlap between different cell types, i.e., that our study of fibroblasts could be of biological relevance. We examined the SOX9 regulatory region by high-resolution ChIP-on-chip experiments (a kind of "chromatin-CGH") and DNA methylation investigations. The RevSex duplication was associated with chromatin changes predicting better accessibility of the SRY-responsive TESCO enhancer region 14-15 kb upstream of SOX9. Four kb downstream of the TESCO evolutionary conserved region, a peak of the enhancer/promoter-associated H3K4me3 mark was found together with a major dip of the repressive H3K9me3 chromatin mark. Similar differences were also found when three control males were compared with three control females. A marked male/female difference was a more open chromatin signature in males starting ~400 kb upstream of SOX9 and increasing toward the SOX9 promoter. In the RevSex duplication-carrier father, two positions of DNA hypomethylation were also found, one corresponding to the H3K4me3 peak mentioned above. Our results suggest that the RevSex duplication could operate by inducing long-range epigenetic changes. Furthermore, the differences in chromatin state maps between males and females suggest that the Y chromosome or X chromosome dosage may affect chromatin conformation, i.e., that sex-dependent gene regulation may take place by chromatin modification.

  8. Empowerment Zones and Enterprise Districts - MDC_NeighborhoodStabilizationProg

    Data.gov (United States)

    NSGIC Local Govt | GIS Inventory — Boundaries of Miami-Dade County's Neighborhood Stabilization Program (NSP) areas. NSP is a HUD program to provide emergency assistance to state and local governments...

  9. s |-|~o RT"? REv

    African Journals Online (AJOL)

    subserving any useful function)”. Tumour arising from any portion of the eyeball either on thesurface or within the eyeball constitutes an ocular neoplasm. Ocular tumours may be benign with a slow propensity to proliferation. Metastasis is rare. However, the function may be compromised due too compression of.

  10. Nuclear Power Plants (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Lyerly, Ray L.; Mitchell III, Walter [Southern Nuclear Engineering, Inc.

    1973-01-01

    Projected energy requirements for the future suggest that we must employ atomic energy to generate electric power or face depletion of our fossil-fuel resources—coal, oil, and gas. In short, both conservation and economic considerations will require us to use nuclear energy to generate the electricity that supports our civilization. Until we reach the time when nuclear power plants are as common as fossil-fueled or hydroelectric plants, many people will wonder how the nuclear plants work, how much they cost, where they are located, and what kinds of reactors they use. The purpose of this booklet is to answer these questions. In doing so, it will consider only central station plants, which are those that provide electric power for established utility systems.

  11. Electricity and Man (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Asimov, Isaac

    1974-01-01

    Our whole industrial civilization depends upon cheap energy streaking out in all directions and under fingertip controls—and that would be very difficult without electric current. It would take enormous ingenuity to find substitutes. If all electricity were shut off, then all electronic devices would be dead. Electric lights would darken. Aluminum would become a rare metal. All factories would have to develop power sources on the spot and would have to switch to primitive, mechanical controls. Every house and every farm would have to give up items we have come to take for granted—not only electric lights, but electric irons, electric toasters, electric water pumps, electric control of furnaces, and so on.

  12. Premixed Supersonic Combustion (Rev)

    Science.gov (United States)

    2015-02-20

    calculated using the 1-D separated flow model for the isolator described in Heiser and Pratt (Ref. 19). Several curves are shown. The green curve corresponds...Report 88-3059, 1988. [18] Krauss, R.H. and McDaniel, J.C., “A Clean Air Continuous Flow Propulsion Facility,” AIAA Report 92-3912, 1992. [19] Heiser

  13. Radiotherapy. 2. rev. ed.

    International Nuclear Information System (INIS)

    Wannenmacher, Michael; Debus, Juergen; Wenz, Frederik

    2013-01-01

    The purpose of this medical specialty book, besides presenting the state of the art in clinical radiotherapy and radiooncology, is to explain the basic principles of medical physics and radiobiology. Following a number of chapters on general topics and theory it provides detailed coverage of the individual organ systems, briefly addressing future aspects in the process. The authors relate their view that radiooncology as a medical specialty will continue to be under pressure to change and that it will take continuous innovation to secure its status within the interdisciplinary context around the treatment of cancer patients. The authors of this, the textbook's second edition, have dedicated much space to modern methods and techniques in order to do justice to these developments.

  14. Whole Body Counters (rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Woodburn, John H. [Walter Johnson High School, Rockville, MD; Lengemann, Frederick W. [Cornell University

    1967-01-01

    Whole body counters are radiation detecting and measuring instruments that provide information about the human body. This booklet describes different whole body counters, scientific principles that are applied to their design, and ways they are used.

  15. Horns Rev offshore wind farm. Introducing hard bottom substrate sea bottom and marine biology. Status report 2001

    International Nuclear Information System (INIS)

    Leonhard, S.B.; Pedersen, John

    2002-08-01

    A baseline description of the benthos was carried out in spring and autumn 2001 prior to the construction of an offshore wind farm at Horns Rev, situated approximately 15 km off Blaevands Huk, which is Denmark's most westerly point. The surveys have been conducted as part of an environmental monitoring programme for the introduction of hard bottom substrates in the North Sea. The establishment of a monitoring programme is required according to some environmental guidelines set up by the Danish Energy Agency for offshore wind farms. Because no environmental criteria existed for benthic communities in connection with the construction activities, no power analysis was made prior to the design of the monitoring programme. The monitoring programme established for the benthic infauna is thus somewhat limited and only major changes in the community structure are expected to be detectable. The baseline description for the benthic infauna can also be used for comparison of the stomach contents of fish in a comparative programme. A newly defined reference area may be introduced for the fish programme why sampling in this area was carried out in the autumn 2001. Samples were recovered at a total of 18 stations at 6 wind turbine locations in the wind farm area in June 2001 and at a total of 9 stations at 3 wind turbine locations in September 2001. In September additional sampling was carried out at 5 stations in a designated reference area. At the wind turbine locations sampling was carried out at 3 stations located 5, 25 and 100 m from the edge of the planned scour protection. Samples were analysed for sediment characteristics and for benthic infauna. Only the benthos relating to the macrofauna was investigated during the surveys. (au)

  16. Revival and emended description of 'Mycobacterium paraffinicum' Davis, Chase and Raymond 1956 as Mycobacterium paraffinicum sp. nov., nom. rev.

    Science.gov (United States)

    Toney, Nadege; Adekambi, Toidi; Toney, Sean; Yakrus, Mitchell; Butler, W Ray

    2010-10-01

    The omission of the name 'Mycobacterium paraffinicum' from the Approved Lists of Bacterial Names was due to phenotypic confusion surrounding a close relationship with Mycobacterium scrofulaceum. Correspondingly, 'M. paraffinicum' strains grew slowly in > 7 days, stained acid-alcohol-fast and produced yellow-pigmented, smooth, waxy colonies in the dark at an optimal temperature of 35°C. However, 'M. paraffinicum' strains demonstrated no activity for urease, nicotinamidase or pyrazinamidase and lacked growth at 42°C, unlike M. scrofulaceum. The mycolic acid pattern, as determined by HPLC, clustered 'M. paraffinicum' with M. scrofulaceum, Mycobacterium avium and Mycobacterium parascrofulaceum. Strains were fully susceptible to linezolid, rifabutin, clarithromycin and amikacin. Examination of the historical reference strain of 'M. paraffinicum', ATCC 12670, and five additional isolates using comparative studies with 16S rRNA, hsp65 and rpoB gene and concatenated sequences showed that they formed a tight taxonomic group that was distinct from similar non-tuberculous mycobacteria. Multilocus enzyme electrophoresis (MEE) analysis confirmed a close association of the five additional isolates with the reference strain of 'M. paraffinicum' with a genetic distance of 0.12 and showed that all six strains were distinct from other closely related species. These genetic results provided unambiguous evidence of the uniqueness of this slowly growing, scotochromogenic species and supported the revival of the name as Mycobacterium paraffinicum (ex Davis, Chase and Raymond 1956) sp. nov., nom. rev. We propose the previously deposited reference strain ATCC 12670(T) =DSM 44181(T) =NCIMB 10420(T), located in collections worldwide, as the type strain.

  17. Horns Rev offshore wind farm. Introducing hard bottom substrate sea bottom and marine biology. Status report 2001

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S.B.; Pedersen, John

    2002-08-15

    A baseline description of the benthos was carried out in spring and autumn 2001 prior to the construction of an offshore wind farm at Horns Rev, situated approximately 15 km off Blaevands Huk, which is Denmark's most westerly point. The surveys have been conducted as part of an environmental monitoring programme for the introduction of hard bottom substrates in the North Sea. The establishment of a monitoring programme is required according to some environmental guidelines set up by the Danish Energy Agency for offshore wind farms. Because no environmental criteria existed for benthic communities in connection with the construction activities, no power analysis was made prior to the design of the monitoring programme. The monitoring programme established for the benthic infauna is thus somewhat limited and only major changes in the community structure are expected to be detectable. The baseline description for the benthic infauna can also be used for comparison of the stomach contents of fish in a comparative programme. A newly defined reference area may be introduced for the fish programme why sampling in this area was carried out in the autumn 2001. Samples were recovered at a total of 18 stations at 6 wind turbine locations in the wind farm area in June 2001 and at a total of 9 stations at 3 wind turbine locations in September 2001. In September additional sampling was carried out at 5 stations in a designated reference area. At the wind turbine locations sampling was carried out at 3 stations located 5, 25 and 100 m from the edge of the planned scour protection. Samples were analysed for sediment characteristics and for benthic infauna. Only the benthos relating to the macrofauna was investigated during the surveys. (au)

  18. Horns Rev offshore wind farm. Introducing hard bottom substrate sea bottom and marine biology. Status report 2001

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S B; Pedersen, John

    2002-08-15

    A baseline description of the benthos was carried out in spring and autumn 2001 prior to the construction of an offshore wind farm at Horns Rev, situated approximately 15 km off Blaevands Huk, which is Denmark's most westerly point. The surveys have been conducted as part of an environmental monitoring programme for the introduction of hard bottom substrates in the North Sea. The establishment of a monitoring programme is required according to some environmental guidelines set up by the Danish Energy Agency for offshore wind farms. Because no environmental criteria existed for benthic communities in connection with the construction activities, no power analysis was made prior to the design of the monitoring programme. The monitoring programme established for the benthic infauna is thus somewhat limited and only major changes in the community structure are expected to be detectable. The baseline description for the benthic infauna can also be used for comparison of the stomach contents of fish in a comparative programme. A newly defined reference area may be introduced for the fish programme why sampling in this area was carried out in the autumn 2001. Samples were recovered at a total of 18 stations at 6 wind turbine locations in the wind farm area in June 2001 and at a total of 9 stations at 3 wind turbine locations in September 2001. In September additional sampling was carried out at 5 stations in a designated reference area. At the wind turbine locations sampling was carried out at 3 stations located 5, 25 and 100 m from the edge of the planned scour protection. Samples were analysed for sediment characteristics and for benthic infauna. Only the benthos relating to the macrofauna was investigated during the surveys. (au)

  19. Final Report - DuraMelter 100 Tests to Support LAW Glass Formulation Correlation Development, VSL-06R6480-1, Rev. 0

    Energy Technology Data Exchange (ETDEWEB)

    Kruger, Albert A.; Muller, I. S.; Gong, W.; Pegg, I. L.; Matlack, K. S.

    2013-12-03

    This report describes the results of work and testing specified by Test Specifications 24590-LAW-TSP-RT-04-004, Rev. 0, Test Plans VSL-05T5480-1, Rev. 0 and Text Exceptions 24590-LAW-TEF-RT-05-00002. The work and any associated testing followed established quality assurance requirements and was conducted as authorized. The descriptions provided in this test report are an accurate account of both the conduct of the work and the data collected. Results required by the Test Plan are reported. Also reported are any unusual or anomalous occurences that are different from the starting hypotheses. The test results and this report have been reviewed and verified.

  20. Administrative circular No.14 (Rev. 3) – Protection of members of the personnel against the financial consequences of illness, accident and incapacity for work

    CERN Multimedia

    2013-01-01

    Administrative Circular No. 14 (Rev. 3) entitled “Protection of members the personnel against the financial consequences of illness, accident and incapacity for work”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 19 April 2012 and entering into force on 1 January 2013, is available on the intranet site of the Human Resources Department.   This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 14 (Rev. 2) entitled “Protection of members of the personnel against the financial consequences of illness, accident and disability” from July 2006. The circular was revised in order to improve the procedure before the Joint Advisory Rehabilitation and Disability Board (JARDB) and the management of long-term sick leave through a multidisciplinary approach launched upstream. The aim of this approach is to allow staff/fellows c...

  1. Administrative Circular No. 2 (Rev. 6) – Recruitment, appointment and possible developments regarding the contractual position of staff members (1 January 2015)

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 2 (Rev. 6) entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 27 November 2014 is available on the Human Resources Department website. It cancels and replaces Administrative Circular No. 2 (Rev. 5) entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members" of September 2011. This circular was revised in order to improve the effectiveness of the career transition measures, in particular by expanding the scope of the programme to include also career transition within the Organization and by placing emphasis on career orientation and job search. Administrative Circular No. 2 will be further revised next year with the adoption of the new contract policy, subject to approval of the relevant amendments by all competent bodies. ...

  2. IMMUNE RESPONSES OF GOATS (SHAMI BREED TO VACCINATION WITH A FULL, REDUCED AND CONJUNCTIVAL DOSE OF BRUCEVAC (BRUCELLA MELITENSIS REV.1 VACCINE

    Directory of Open Access Journals (Sweden)

    F. ALDOMY, M. ALKHAWALDEH1 AND I. B. YOUNIS

    2009-10-01

    Full Text Available Three groups of Shami goats were randomly vaccinated with Brucevac (Rev. 1 vaccine. Group 1 was vaccinated subcutaneously with a full dose (1.54 x 109 organisms. Group 2 was vaccinated conjunctively with one eye drop (5.2 x 108 organisms, while Group 3 was injected subcutaneously with a reduced dose (7.1 x 105 organisms of vaccine. Blood samples were collected before vaccination, two, four, eight, 15 and 24 weeks post vaccination. All samples were tested through CFT, ELISA, SAT and Rose Bengal plate test. All serological tests used detected a higher percentage of vaccinated female kids with a full dose than they did in other groups vaccinated with a reduced dose or with a conjunctival dose of Rev.1 vaccine. The overall results suggested that 100% of animals vaccinated with a conjunctival dose became positive to CFT at two, four, eight and 15 weeks post vaccination, and then the percentage of seropositive animals declined and became 20% at 24 weeks post inoculation. The conjunctival route of vaccination significantly reduced the intensity and duration of the post vaccination serological response, which makes the use of this vaccine compatible with brucellosis programmes, even when these are based on a test-and–slaughter policy. The overall results showed that Shami goats responded to Rev.1 vaccine in the expected way. The majority of animals were seropositive to the CFT by two weeks after vaccination with higher numbers of seropositive animals in the kids group vaccinated with a full dose of Rev.1 vaccine.

  3. Review report 2004. The Danish offshore wind farm demonstration project: Horns Rev and Nysted offshore wind farms. Environmental impact assessment and monitoring

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-10-15

    The present review report presents the two wind farms Horns Rev and Nysted with regard to environmental characteristics and the results of the environmental studies carried out in connection with the Environmental Impact Assessment (EIA) and the baseline and monitoring programmes at the two sites. The descriptions of the programmes are based on extracts from the individual annual reports. Detailed information on method, programmes and conclusions can be found in these reports (see Appendix 1). This review report is divided into two parts. The first part contains a description of the potential environmental impacts of offshore wind farms and the potentially affected part of the environment. The second part includes a description of Horns Rev and Nysted Offshore Wind Farm and the environmental studies carried out here until the end of 2003. Appendix I is a list of the literature published on the studies carried out at Horns Rev and Nysted Offshore Wind Farm. Appendix II is a list of some of the literature relevant to the demonstration projects but not directly related to the projects. The offshore wind farm situated in the Roedsand area is officially denoted Nysted Offshore Wind Farm, and in the present report the denotation 'Nysted' will be used as well when referring to Nysted Offshore Wind Farm. The denotation Roedsand will be used when referring to the actual area Roedsand. (au)

  4. Finanšu pārskata revīzija un tās gaita uzņēmumā SIA “X”

    OpenAIRE

    Pocjus, Evita

    2016-01-01

    Diplomdarba tēma: „Finanšu pārskata revīzija un tās gaita uzņēmumā SIA „X”” Finanšu pārskata revīzija ir būtiska, jo arī Latvijas Zvērināto revidentu asociācijas apkopotie statistikas dati norāda, ka Latvijā ~90% no pirmreizēji revidētiem uzņēmumiem tiek atklātas būtiskas neatbilstības grāmatvedības uzskaitē, tās atbilstībai LR likumiem un normatīviem aktiem, kā arī finanšu pārskata sagatavošanā. Finanšu pārskata revīzija ir nepieciešama katram uzņēmumam, kurš grib iegūt klientu un sadarbība...

  5. Review report 2003. The Danish offshore wind farm demonstration project: Horns Rev and Nysted offshore wind farms. Environmental impact assessment and monitoring

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-09-15

    The present review report presents the two wind farms Homs Rev and Nysted, with regard to environmental characteristics and the results of the environmental studies carried out in connection with the Environmental Impact Assessment (EIA) and the baseline and monitoring programmes at the two sites. The descriptions of the programmes are based on extracts from the individual annual reports. Detailed information on methods, programmes and conclusions can be found in these reports. This review report is divided into two parts. The first part contains a description of the potential environmentally impacts of offshore wind farms and the potentially affected part of the environment. The second part includes a description of Horns Rev and Nysted Offshore Wind Farm and the environmental studies carried out here until the end of 2003. Appendix I is a list of the literature published on the studies carried out at Hors Rev and Nysted Offshore Wind Farm. Appendix II is a list of some of the literature relevant to the demonstration projects but not directly related to the projects. The offshore wind farm situated at the bdsand area is officially denoted Nysted Offshore Wind Farm, and in the present report the denotation 'Nysted' will be used as well when referring to Nysted Offshore Wind Farm. The denotation Roedsand will be used when referring to the actual area Roedsand. (au)

  6. Review report 2004. The Danish offshore wind farm demonstration project: Horns Rev and Nysted offshore wind farms. Environmental impact assessment and monitoring

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-10-15

    The present review report presents the two wind farms Horns Rev and Nysted with regard to environmental characteristics and the results of the environmental studies carried out in connection with the Environmental Impact Assessment (EIA) and the baseline and monitoring programmes at the two sites. The descriptions of the programmes are based on extracts from the individual annual reports. Detailed information on method, programmes and conclusions can be found in these reports (see Appendix 1). This review report is divided into two parts. The first part contains a description of the potential environmental impacts of offshore wind farms and the potentially affected part of the environment. The second part includes a description of Horns Rev and Nysted Offshore Wind Farm and the environmental studies carried out here until the end of 2003. Appendix I is a list of the literature published on the studies carried out at Horns Rev and Nysted Offshore Wind Farm. Appendix II is a list of some of the literature relevant to the demonstration projects but not directly related to the projects. The offshore wind farm situated in the Roedsand area is officially denoted Nysted Offshore Wind Farm, and in the present report the denotation 'Nysted' will be used as well when referring to Nysted Offshore Wind Farm. The denotation Roedsand will be used when referring to the actual area Roedsand. (au)

  7. Estimativas de parâmetros genéticos e ganhos de seleção em progênies de polinização aberta de açaizeiro Estimates of genetic parameters and selection gains in progenyes of open pollination of açai tree (Euterpe oleracea

    Directory of Open Access Journals (Sweden)

    João Tomé de Farias Neto

    2008-12-01

    Full Text Available O presente trabalho teve como objetivos estudar a variabilidade genética, estimar parâmetros genéticos e fenotípicos, e realizar a predição de valores genéticos dos indivíduos de açaizeiro irrigado no Estado do Pará, utilizando a metodologia BLUP/REML a partir da avaliação de progênies com base nos caracteres altura do primeiro cacho (APC, peso total do cacho (PTC, peso total de frutos (PTF, número de cachos (NC, peso médio do cacho (PMC, comprimento médio da ráquis (CMR, número médio de ráquilas (NMR, número de perfilhos (NP e peso médio de cem frutos (P100. Cinqüenta progênies foram avaliadas em dois látices 5 x 5 com duas repetições e parcelas lineares de cinco plantas cada, no espaçamento de 5 m x 5 m. O programa computacional Selegen- Reml/Blup foi utilizado para as análises genéticas e a identificação dos melhores indivíduos para compor a população de produção de sementes para um programa a curto prazo e a de melhoramento para um programa a longo prazo. As correlações genotípicas de maiores magnitudes foram aquelas envolvendo o peso total de frutos e peso total do cacho, peso total do cacho e número de cacho e peso total de fruto e número de cacho, indicando que a seleção para peso total de frutos pode ser realizada por meio da seleção daquela de mais fácil seleção. As estimativas dos parâmetros genéticos obtidos revelam excelente potencial seletivo da população e variabilidade genética suficiente para o melhoramento genético da população a curto e longo prazos. Ganho genético considerável de 45,33% em relação à média do experimento pode ser obtido com a seleção dos 20 melhores indivíduos para o caráter produção total de frutos.The present work aimed to study the genetic variability, estimate the genetic and phenotypic parameters and to conduct the prediction of genetic values of individuals of irrigated açai tree in the state of Pará. The objective was the selection

  8. Genetic evaluation and deviance analysis in an unbalanced provenance and progeny test of maté Avaliação genética e análise de deviance em um teste desbalanceado de procedência e progênie de Ilex paraguariensis

    Directory of Open Access Journals (Sweden)

    José Alfredo Sturion

    2010-10-01

    -pagination:widow-orphan; font-size:10.0pt; mso-bidi-font-size:12.0pt; font-family:Arial; mso-fareast-font-family:"Times New Roman"; font-weight:bold;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} -->

    Em um teste combinado de procedência e progênie de erva-mate (Ilex paraguariensis A. St. Hil., os efeitos aleatórios do modelo foram testados pelo teste da razão de verossimilhança (LTR. Os dados referem-se
    ao peso de biomassa foliar obtido aos seis anos de idade, por ocasião da terceira poda, em um teste com
    sete procedências e 140 progênies de meios-irmãos instalado no delineamento de blocos ao acaso, com dez repetições de parcelas lineares com seis plantas no espaçamento de 3 m x 2 m. A análise de deviance (Anadev foi realizada e as seguintes conclusões foram obtidas: os componentes de variância associados a todos os efeitos aleatórios do modelo e respectivos coeficientes de determinação (herdabilidades dos efeitos genéticos aditivos, dos efeitos de parcela e dos efeitos de procedência foram significativamente diferentes de zero, indicando que existe variabilidade genética significativa a ser explorada pelo melhoramento, tanto entre como dentro de populações naturais.

    doi: 10.4336/2010.pfb.30.62.157

  9. Situation analysis of physical independence of the equipment and safety circuits of Almaraz NPP regarding R.G. 1.75 rev.3 (2005); Analisis de la situacion de la independencia fisica de los equipos y circuitos electricos de seguridad de C. N. Almaraz respecto a la R. G. 1.75 rev 3 (2005)

    Energy Technology Data Exchange (ETDEWEB)

    Seijas Portela, S.

    2010-07-01

    Situation analysis of physical independence of the electrical equipment and circuits CN safety Almaraz about R.G. 1.75 rev. 3. (2005) The aim of this paper is to present the work done in the analysis of the physical separation of redundant safety electrical equipment (emergency diesel generators, medium voltage, electrical cabinets, etc.) and physical separation of circuits and electrical conduits.

  10. Elsam. Offshore Wind Farm. Horns Rev. Annual status report for the environmental monitoring programme 1. January 2002 - 31. December 2002

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-04-15

    This annual report, which is a baseline and/or an evaluation of the effects of construction phase, is based on extracts from our consultants' annual reports. These annual reports are individual reports, in which surveys have been made during the year 2002 and reported in the beginning of 2003. Detailed information on methods, programmes and conclusions can be found in these reports. In general it should be noted that apparently the construction phase has not had any unintended effects on the parameters surveyed. Therefore we can also conclude that the protective measures established by Elsam, especially scaring devices/ramp-ups for marine mammals, have had the intended effect. Especially interesting parameters that should be mentioned are: Birds: The seagull species 'Herring Gulls' was found to be attracted by the wind farm during the construction period. The number of Divers has decreased, however statistically this decrease is very small as very few Divers have been observed under the baseline. Porpoises: Porpoises generally stayed away from the construction area during pile driving. This effect was intended and scaring devices were deployed in order to ensure that the animals' hearing was not permanently damaged. Seals: The purpose of the programme has not been to show statistically significant effects during the construction phase. The purpose was to get an idea of their behaviour during the construction phase within the wind farm as well as on the reef. It has been concluded that Horns Rev is not used as a foraging area as much as expected. It is used as transit area for bigger foraging sites in the North Sea. Sand eels and Spisula: A baseline survey of sand eels within the wind farm area has been made. It is shown that sand eels are found in the wind farm area. Spisula have been found in the wind farm area but not in the amount expected. Hard substrate habitat: Baseline surveys on fish have been made during the spring of 2002. The

  11. Elsam. Offshore Wind Farm. Horns Rev. Annual status report for the environmental monitoring programme 1. January 2002 - 31. December 2002

    International Nuclear Information System (INIS)

    2003-04-01

    This annual report, which is a baseline and/or an evaluation of the effects of construction phase, is based on extracts from our consultants' annual reports. These annual reports are individual reports, in which surveys have been made during the year 2002 and reported in the beginning of 2003. Detailed information on methods, programmes and conclusions can be found in these reports. In general it should be noted that apparently the construction phase has not had any unintended effects on the parameters surveyed. Therefore we can also conclude that the protective measures established by Elsam, especially scaring devices/ramp-ups for marine mammals, have had the intended effect. Especially interesting parameters that should be mentioned are: Birds: The seagull species 'Herring Gulls' was found to be attracted by the wind farm during the construction period. The number of Divers has decreased, however statistically this decrease is very small as very few Divers have been observed under the baseline. Porpoises: Porpoises generally stayed away from the construction area during pile driving. This effect was intended and scaring devices were deployed in order to ensure that the animals' hearing was not permanently damaged. Seals: The purpose of the programme has not been to show statistically significant effects during the construction phase. The purpose was to get an idea of their behaviour during the construction phase within the wind farm as well as on the reef. It has been concluded that Horns Rev is not used as a foraging area as much as expected. It is used as transit area for bigger foraging sites in the North Sea. Sand eels and Spisula: A baseline survey of sand eels within the wind farm area has been made. It is shown that sand eels are found in the wind farm area. Spisula have been found in the wind farm area but not in the amount expected. Hard substrate habitat: Baseline surveys on fish have been made during the spring of 2002. The programme regarding the

  12. Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae. IV. Influence of DNA replication and excision repair on REV2 dependent UV-mutagenesis and repair

    Energy Technology Data Exchange (ETDEWEB)

    Siede, W.; Eckardt, F.

    1986-01-01

    A double mutant being thermoconditionally defective in mutation induction as well as in repair of pre-lethal UV-induced DNA damage (rev2ts) and deficient in excision repair (rad3-2) was studied in temperature-shift experiments. The influence of inhibitors of DNA replication (hydroxyurea, aphidicolin) was determined. Additionally, an analysis of the dose-response pattern of mutation induction (mutation kinetics) at several ochre alleles was carried out. It was concluded that the UV-inducible REV2 dependent mutagenic repair process is not induced in excision-deficient cells. In excision-deficient cells, REV2 dependent mutation fixation is slow and mostly post-replicative though not dependent on DNA replication. The REV2 mediated mutagenic process could be separated from the repair function.

  13. Mr Huidong LI Deputy Chairman and Secretary-General, Rev. Committee of Chinese Nationalist Party Vice President, Sun Yat-Sen Culture Interchange Association of China Member, Committee for Internal and Judicial Affairs of the National People's Congress of China

    CERN Multimedia

    Bennett, Sophia Elizabeth

    2017-01-01

    Mr Huidong LI Deputy Chairman and Secretary-General, Rev. Committee of Chinese Nationalist Party Vice President, Sun Yat-Sen Culture Interchange Association of China Member, Committee for Internal and Judicial Affairs of the National People's Congress of China

  14. Grate Pallet 8232 (GP-8232) Vehicle Pallet, Evaluation Tests MIL-STD-1660, "Design Criteria for Ammunition Unit Loads" and TP-94-01 (REV 1), "Transportability Testing Procedures"

    National Research Council Canada - National Science Library

    Barickman, Philip

    2003-01-01

    .... The testing was conducted for informational purposes only. The GP-8232 Vehicle Pallet was evaluated by the testing procedures set forth in MIL-STD-1660 and TP-94-01 (Rev. 1) testing procedures...

  15. The role of hRev7, the accessory subunit of hPolζ, in translesion synthesis past DNA damage induced by benzo[a]pyrene diol epoxide (BPDE

    Directory of Open Access Journals (Sweden)

    Maher Veronica M

    2010-12-01

    Full Text Available Abstract Background DNA polymerase zeta (Polζ is a specialized DNA polymerase that, unlike classical replicative polymerases, is capable of replicating past DNA lesions, i.e. of performing translesion synthesis (TLS. The catalytic subunit of hPolζ, hRev3, has been shown to play a critical role in DNA damage-induced mutagenesis in human cells, but less is known about the role of hRev7, the accessory subunit of hPolζ, in such mutagenesis. To address this question, we recently generated human fibroblasts with very significantly reduced levels of hRev7 protein and demonstrated that hRev7 is required to protect cells from ultraviolet(254 nm (UV radiation-induced cytotoxicity and mutagenesis (McNally et al., DNA Repair 7 (2008 597-604. The goal of the present study was to determine whether hRev7 is similarly involved in the tolerance of DNA damage induced by benzo[a]pyrene diol epoxide (BPDE, the reactive form of the widespread environmental carcinogen benzo[a]pyrene. Methods To determine whether hRev7 also plays a role in protecting human cells from the cytotoxicity and mutagenesis induced by benzo[a]pyrene diol epoxide (BPDE, cell strains with reduced hRev7 were compared to their parental strain and a vector control strain for the effect of BPDE on cell survival, induction of mutations, and the ability to progress through the cell cycle. Results The results show that cell strains with reduced hRev7 are more sensitive to the cytotoxic effect of BPDE than the control strains, and progress through S-phase at a slower rate than the control cells following BPDE treatment, indicating that hRev7, and likely hPolζ, is required for efficient bypass of BPDE-induced DNA lesions. However, neither the frequency nor kinds of mutations induced by BPDE in cells with reduced hRev7 differ significantly from those induced in the control strains, suggesting that hPolζ is not essential for inserting nucleotides opposite BPDE-induced DNA damage. Conclusions Taken

  16. Genetic parameter and correlation estimates of processing traits in half-sib progenies of tropical-adapted carrot germplasm Parâmetros genéticos e correlações entre características para processamento em progênies de meios-irmãos de germoplasma de cenoura tropical

    Directory of Open Access Journals (Sweden)

    Jairo V Vieira

    2012-03-01

    Full Text Available The estimate of the genetic parameters associated with processing (fresh-cut traits, including root length (RL, is crucial for carrot breeding programs in tropical areas. The cultivar Alvorada is an important germplasm due to its resistance to nematodes, leaf blight, heat-tolerance, and high carotenoid content. Seventy-four 'Alvorada' half-sib progenies were evaluated during the summer of 2005 in the Federal District, Brazil, in a randomized complete block design with three replications. Thirteen competitive plants in each block were randomly selected and evaluated and/or classified for RL and for number of leaves (NL, leaf length (LL, root tip type (RT, root mass (RW, crown shape (CS, root diameter (RD, and xylem diameter (XD. The Pearson's correlation coefficients and the heritability values were estimated for all traits. The path analysis was also used considering the RL trait as dependent variable. The heritability for RL ranged from 12 to 44%. For the other traits, the values ranged from 3% (RD to 79% (LL. Phenotypic and genotypic correlations among all traits were low to intermediate. Path analysis indicated positive direct relationship between RL and RW, whereas RD and XD displayed negative direct effect on RL. Longer roots had narrow diameter and narrow XD. Recurrent selection based upon either half-sib or S1 families would be more effective than mass phenotypic recurrent selection in increasing RL and to develop populations expressing multiple desirable processing traits in tropical-adapted carrot germplasm.A estimativa de parâmetros genéticos associados com caracteres de processamento industrial, incluindo comprimento de raiz (RL, é crucial para programas de melhoramento de cenoura para áreas tropicais. A cultivar Alvorada é um importante germoplasma devido à sua resistência a nematóides e queima-das-folhas, tolerância ao calor e alto conteúdo de carotenóides. Setenta e quatro progênies meio-irmãs derivadas de 'Alvorada

  17. Reação de híbridos, linhagens e progênies de pimentão à requeima causada por Phytophthora capsici e ao mosaico amarelo causado por Pepper yellow mosaic vírus (PepYMV Reaction of hybrids, lines and progenies of sweet pepper the blight caused by Phytophthora capsici and to Pepper yellow mosaic virus (PepYMV

    Directory of Open Access Journals (Sweden)

    Ildon Rodrigues do Nascimento

    2007-02-01

    Full Text Available A requeima do pimentão (Capsicum annuum L. causada por Phytophthora capsici e o mosaico amarelo causado por PepYMV são prioridades nos programas de melhoramento de pimentão em andamento no Brasil. Foram avaliados três híbridos comerciais (Magali R, Atenas F1 e Fortuna Super F1, cinco linhagens, 20 híbridos experimentais e duas progênies F2:4 do programa de melhoramento da Universidade Federal de Lavras/HortiAgro Sementes quanto à reação a P. capsici e a PepYMV. Os experimentos foram montados independentemente em blocos casualizados, com quatro e duas repetições, respectivamente. Cada parcela foi constituída de oito plantas instaladas em bandejas de 128 células, que foram mantidas em estufa com cobertura plástica e laterais teladas. As avaliações foram feitas do 4º ao 14º e do 15º ao 40º dias após a inoculação (DAI para P. capsici e PepYMV, respectivamente. A inoculação com P. capsici foi feita pormeio da distribuição de 5 mL de suspensão com 10(4 zoósporos/mL no solo ao redor do colo de cada planta. A inoculação mecânica de PepYMV foi feita a partir de macerados de folhas de Nicotiana tabacum cv. TNN, previamente infectadas. Os híbridos Magali R e Fortuna Super, foram suscetíveis a P. capsici, enquanto que o acesso Criollo de Morellos 334, a linhagem PIM-013 e as progênies PIX-03 pl#03-2 e PIX-030 pl#06-3 (ambas originadas do cruzamento com Criollo de Morellos, foram resistentes. Reação de resistência a P. capsici foi também observada para os híbridos experimentais que tiveram PIM-013 como uma das linhagens parentais. Criollo de Morellos 334, as progênies PIX-03 pl#03-2 e PIX-030 pl#06-3, o híbrido comercial Magali R e outros 6 híbridos experimentais que tinham como um dos genitores a linhagem MYR-29 forma ressitentes ao PepYMV. As progênies PIX-03 pl#03-2 e PIX-030 pl#06-3 são fontes promissoras de resistência a ambos os patógenos para serem desenvolvidas e exploradas em programas de melhoramento

  18. Codon optimization of the HIV-1 vpu and vif genes stabilizes their mRNA and allows for highly efficient Rev-independent expression

    International Nuclear Information System (INIS)

    Nguyen, Kim-Lien; Llano, Manuel; Akari, Hirofumi; Miyagi, Eri; Poeschla, Eric M.; Strebel, Klaus; Bour, Stephan

    2004-01-01

    Two HIV-1 accessory proteins, Vpu and Vif, are notoriously difficult to express autonomously in the absence of the viral Tat and Rev proteins. We examined whether the codon bias observed in the vpu and vif genes relative to highly expressed human genes contributes to the Rev dependence and low expression level outside the context of the viral genome. The entire vpu gene as well as the 5' half of the vif gene were codon optimized and the resulting open reading frames (ORFs) (vphu and hvif, respectively) were cloned in autonomous expression vectors under the transcriptional control of the CMV promoter. Codon optimization efficiently removed the expression block observed in the native genes and allowed high levels of Rev- and Tat-independent expression of Vpu and Vif. Most of the higher protein levels detected are accounted for by enhanced steady-state levels of the mRNA encoding the optimized species. Nuclear run-on experiments show for the first time that codon optimization has no effect on the rate of transcriptional initiation or elongation of the vphu mRNA. Instead, optimization of the vpu gene was found to stabilize the vphu mRNA in the nucleus and enhance its export to the cytoplasm. This was achieved by allowing the optimized mRNA to use a new CRM1-independent nuclear export pathway. This work provides a better understanding of the molecular mechanisms underlying the process of codon optimization and introduces novel tools to study the biological functions of the Vpu and Vif proteins independently of other viral proteins

  19. Characterization of an internal ribosomal entry segment within the 5' leader of avian reticuloendotheliosis virus type A RNA and development of novel MLV-REV-based retroviral vectors.

    Science.gov (United States)

    López-Lastra, M; Gabus, C; Darlix, J L

    1997-11-01

    The murine leukemia virus (MLV)-related type C viruses constitute a major class of retroviruses that includes numerous endogenous and exogenous mammalian viruses and the related avian spleen necrosis virus (SNV). The MLV-related viruses possess a long and multifunctional 5' untranslated leader involved in key steps of the viral life cycle--splicing, translation, RNA dimerization, encapsidation, and reverse transcription. Recent studies have shown that the 5' leader of Friend murine leukemia virus and Moloney murine leukemia virus can direct cap independent translation of gag precursor proteins (Berlioz et al., 1995; Vagner et al., 1995b). These data, together with structural homology studies (Koning et al., 1992), prompted us to undertake a search for new internal ribosome entry segment (IRES) of retroviral origin. Here we describe an IRES element within the 5' leader of avian reticuloendotheliosis virus type A (REV-A) genomic RNA. Data show that the REV-A 5' IRES element maps downstream of the packaging/dimerization (E/DLS) sequence (Watanabe and Temin, 1982; Darlix et al., 1992) and the minimal IRES sequence appears to be within a 129 nt fragment (nucleotides 452-580) of the 5' leader, immediately upstream of the gag AUG codon. The REV-A IRES has been successfully utilized in the construction of novel high titer MLV-based retroviral vectors, containing one or more IRES elements of retroviral origin. These retroviral constructs, which represent a starting point for the design of novel vectors suitable for gene therapy, are also of interest as a model system of internal translation initiation and its possible regulation during development, cancer, or virus infection.

  20. Base-line investigations of birds in relation to an offshore wind farm at Horns Rev, and results from the year of construction

    International Nuclear Information System (INIS)

    Kjaer Christensen, T.; Clausager, I.; Krag Petersen, I.

    2003-01-01

    The present report presents the base-line investigations of birds conducted during August 1999-April 2001 in relation to construction of an offshore wind farm at Horns Rev, 14 km west-south-west of Blaevandshuk, in the Danish North Sea. The report also presents data collected during the period September 2001-April 2002, when construction of the wind farm was in progress. The wind farm will consist of 80 wind turbines, each of 2 MW, and cover an area of c. 20 km 2 . The eastern part of the North Sea constitutes major staging and wintering grounds for huge numbers of water- and seabirds. The area is also known to be an important site for migrating birds, which especially in autumn pass in large numbers. As Denmark has obligations to protect and maintain the bird populations it was laid down in the approval for erection of the wind farm that the impact on birds should be investigated. According to the 1% criteria defined in the Ramsar Convention, the eastern part of the North Sea including the wind farm area is of international importance to divers, Common Tern, and Sandwich Tern. A number of other species, e.g. Common Eider, Common Scoter, Guillemot and Razorbill, are present in the area in significant numbers as well, though these numbers do not make up 1% of the populations. Detailed distributions of birds in the area around and at Horns Rev were virtually unknown until initiation of this project. Previous bird counts in this area have been carried out almost exclusively from the coast and detailed knowledge exists concerning the numbers of roosting at and migrating birds from the westernmost point of Jutland, Blaevandshuk. To describe the numbers and distributions of birds staging and wintering in the Horns Rev area, bird investigations were initiated in 1999 by using standardised transect counts from aircraft. Up to April 2002, 18 aerial counts have been carried out over an area of c. 1,700 km 2 centred on the Horns Rev project area. (au)

  1. Base-line investigations of birds in relation to an offshore wind farm at Horns Rev, and results from the year of construction

    Energy Technology Data Exchange (ETDEWEB)

    Kjaer Christensen, T.; Clausager, I.; Krag Petersen, I.

    2003-04-15

    The present report presents the base-line investigations of birds conducted during August 1999-April 2001 in relation to construction of an offshore wind farm at Horns Rev, 14 km west-south-west of Blaevandshuk, in the Danish North Sea. The report also presents data collected during the period September 2001-April 2002, when construction of the wind farm was in progress. The wind farm will consist of 80 wind turbines, each of 2 MW, and cover an area of c. 20 km{sup 2}. The eastern part of the North Sea constitutes major staging and wintering grounds for huge numbers of water- and seabirds. The area is also known to be an important site for migrating birds, which especially in autumn pass in large numbers. As Denmark has obligations to protect and maintain the bird populations it was laid down in the approval for erection of the wind farm that the impact on birds should be investigated. According to the 1% criteria defined in the Ramsar Convention, the eastern part of the North Sea including the wind farm area is of international importance to divers, Common Tern, and Sandwich Tern. A number of other species, e.g. Common Eider, Common Scoter, Guillemot and Razorbill, are present in the area in significant numbers as well, though these numbers do not make up 1% of the populations. Detailed distributions of birds in the area around and at Horns Rev were virtually unknown until initiation of this project. Previous bird counts in this area have been carried out almost exclusively from the coast and detailed knowledge exists concerning the numbers of roosting at and migrating birds from the westernmost point of Jutland, Blaevandshuk. To describe the numbers and distributions of birds staging and wintering in the Horns Rev area, bird investigations were initiated in 1999 by using standardised transect counts from aircraft. Up to April 2002, 18 aerial counts have been carried out over an area of c. 1,700 km{sup 2} centred on the Horns Rev project area. (au)

  2. Evaluación de dos bombas hidráulicas de engranajes de desplazamientos 32 y 50 cm3/rev

    OpenAIRE

    Pedro Paneque Rondón; Eugenio Romantchik Kriuskova; Luciano Pérez Sobrevilla; Remigio Durán Hernández; Ricardo Castillo Calderín

    2013-01-01

    Como parte de las investigaciones que se llevan a cabo en la Universidad Agraria de La Habana (UNAH), en el Centro de Mecanización Agro- pecuaria (CEMA), con la cooperación de la Universidad Autónoma Chapingo (UACh), en el Departamento de Ingeniería Mecánica Agrícola (DIMA), dirigidas al desarrollo de la Oleohidráulica, se sometieron a evaluación dos bombas de engranajes de la marca Caproni de fabricación búlgara, con desplazamientos o cilindradas de 32 y 50 cm3/rev, refiriéndose la prueba a ...

  3. Anticoking Coatings for High Temperature Petrochemical Reactors Revêtements pour réacteurs pétrochimiques à cokage réduit

    Directory of Open Access Journals (Sweden)

    Ropital F.

    2006-12-01

    Full Text Available Coke deposition is a major problem for several refinery and petrochemical processes, among which steam cracking for ethylene production. Decreasing coking rates will reduce decoking frequency and will also decrease heat transfer degradation. For these processes, a means of reducing coking phenomena is the application of anticoking coatings on the reactor walls. For this purpose a methodology was developed under a CEC Brite-Euram project with steam cracking as the main application. A first selection of the coatings and their techniques of deposition was performed according to their composition (non catalytic compounds, their resistance to carburization and oxidation and also according to the nature of the substrate. The feasibility of coating deposition was studied for small diameter furnace tubes and the techniques were perfected in particular with respect to the physicochemical characterization of the coatings and their resistance during thermal cycling and coking-decoking cycles. For the thermal cycling and the coking evaluation, industrial conditions were reproduced as closely as possible. For coking tests, a microreactor with complete mixing of the gas phase was developed for temperatures up to 950°C. The coking behavior of the coated samples was compared with conventional refractory alloys: reduction of the coking rate by a factor of 3 was measured with the most promising coating. This work was completed by the kinetic modelling of coking on uncoated materials. La formation de coke est un problème crucial pour de nombreux procédés pétrochimiques. Ralentir le cokage permet de réduire la fréquence des décokages et de limiter la dégradation du transfert thermique. Un des moyens envisageables pour réduire le cokage est le dépôt d'un revêtement à la paroi de ces réacteurs. La mise au point de tels revêtements a fait l'objet d'un projet de recherche européen Brite-Euram, pour lequel le vapocraquage a été la principale

  4. Model-based investigation of the circadian clock and cell cycle coupling in mouse embryonic fibroblasts: Prediction of RevErb-α up-regulation during mitosis.

    Science.gov (United States)

    Traynard, Pauline; Feillet, Céline; Soliman, Sylvain; Delaunay, Franck; Fages, François

    2016-11-01

    Experimental observations have put in evidence autonomous self-sustained circadian oscillators in most mammalian cells, and proved the existence of molecular links between the circadian clock and the cell cycle. Some mathematical models have also been built to assess conditions of control of the cell cycle by the circadian clock. However, recent studies in individual NIH3T3 fibroblasts have shown an unexpected acceleration of the circadian clock together with the cell cycle when the culture medium is enriched with growth factors, and the absence of such acceleration in confluent cells. In order to explain these observations, we study a possible entrainment of the circadian clock by the cell cycle through a regulation of clock genes around the mitosis phase. We develop a computational model and a formal specification of the observed behavior to investigate the conditions of entrainment in period and phase. We show that either the selective activation of RevErb-α or the selective inhibition of Bmal1 transcription during the mitosis phase, allow us to fit the experimental data on both period and phase, while a uniform inhibition of transcription during mitosis seems incompatible with the phase data. We conclude on the arguments favoring the RevErb-α up-regulation hypothesis and on some further predictions of the model. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  5. Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERBα/β activation in aryl hydrocarbon receptor-elicited hepatotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Fader, Kelly A.; Nault, Rance [Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI 48824 (United States); Institute for Integrative Toxicology, Michigan State University, East Lansing, MI 48824 (United States); Kirby, Mathew P.; Markous, Gena [Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI 48824 (United States); Matthews, Jason [Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo 0316 (Norway); Zacharewski, Timothy R., E-mail: tzachare@msu.edu [Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI 48824 (United States); Institute for Integrative Toxicology, Michigan State University, East Lansing, MI 48824 (United States)

    2017-04-15

    Persistent aryl hydrocarbon receptor (AhR) agonists elicit dose-dependent hepatic lipid accumulation, oxidative stress, inflammation, and fibrosis in mice. Iron (Fe) promotes AhR-mediated oxidative stress by catalyzing reactive oxygen species (ROS) production. To further characterize the role of Fe in AhR-mediated hepatotoxicity, male C57BL/6 mice were orally gavaged with sesame oil vehicle or 0.01–30 μg/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) every 4 days for 28 days. Duodenal epithelial and hepatic RNA-Seq data were integrated with hepatic AhR ChIP-Seq, capillary electrophoresis protein measurements, and clinical chemistry analyses. TCDD dose-dependently repressed hepatic expression of hepcidin (Hamp and Hamp2), the master regulator of systemic Fe homeostasis, resulting in a 2.6-fold increase in serum Fe with accumulating Fe spilling into urine. Total hepatic Fe levels were negligibly increased while transferrin saturation remained unchanged. Furthermore, TCDD elicited dose-dependent gene expression changes in heme biosynthesis including the induction of aminolevulinic acid synthase 1 (Alas1) and repression of uroporphyrinogen decarboxylase (Urod), leading to a 50% increase in hepatic hemin and a 13.2-fold increase in total urinary porphyrins. Consistent with this heme accumulation, differential gene expression suggests that heme activated BACH1 and REV-ERBα/β, causing induction of heme oxygenase 1 (Hmox1) and repression of fatty acid biosynthesis, respectively. Collectively, these results suggest that Hamp repression, Fe accumulation, and increased heme levels converge to promote oxidative stress and the progression of TCDD-elicited hepatotoxicity. - Highlights: • TCDD represses hepatic hepcidin expression, leading to systemic iron overloading. • Dysregulation of heme biosynthesis is consistent with heme and porphyrin accumulation. • Heme-activated REV-ERBα/β repress circadian-regulated hepatic lipid metabolism. • Disruption of iron

  6. Dosimetric characterization of model Cs-1 Rev2 cesium-131 brachytherapy source in water phantoms and human tissues with MCNP5 Monte Carlo simulation

    International Nuclear Information System (INIS)

    Wang Jianhua; Zhang Hualin

    2008-01-01

    A recently developed alternative brachytherapy seed, Cs-1 Rev2 cesium-131, has begun to be used in clinical practice. The dosimetric characteristics of this source in various media, particularly in human tissues, have not been fully evaluated. The aim of this study was to calculate the dosimetric parameters for the Cs-1 Rev2 cesium-131 seed following the recommendations of the AAPM TG-43U1 report [Rivard et al., Med. Phys. 31, 633-674 (2004)] for new sources in brachytherapy applications. Dose rate constants, radial dose functions, and anisotropy functions of the source in water, Virtual Water, and relevant human soft tissues were calculated using MCNP5 Monte Carlo simulations following the TG-43U1 formalism. The results yielded dose rate constants of 1.048, 1.024, 1.041, and 1.044 cGy h -1 U -1 in water, Virtual Water, muscle, and prostate tissue, respectively. The conversion factor for this new source between water and Virtual Water was 1.02, between muscle and water was 1.006, and between prostate and water was 1.004. The authors' calculation of anisotropy functions in a Virtual Water phantom agreed closely with Murphy's measurements [Murphy et al., Med. Phys. 31, 1529-1538 (2004)]. Our calculations of the radial dose function in water and Virtual Water have good agreement with those in previous experimental and Monte Carlo studies. The TG-43U1 parameters for clinical applications in water, muscle, and prostate tissue are presented in this work

  7. Guidance and considerations for the implementation of INFCIRC/225/Rev.4, the physical protection of nuclear material and nuclear facilities; Orientaciones y sugerencias para la aplicacion del documento INFCIRC/225/Rev.4, proteccion fisica de los materiales y las instalaciones nucleares

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-05-01

    This publication is intended to provide guidance and considerations for a State's competent authority to better understand and prescribe appropriate requirements, consistent with INFCIRC/225/Rev.4 for the protection of nuclear material and nuclear facilities which are compatible with accepted international practice. This report, together with a more detailed report, Handbook on the Physical Protection of Nuclear Material and Facilities, which addresses to the licensee or designer of physical protection systems who has specific implementation and compliance responsibilities, should be used in conjunction to each other to provide better and comprehensive guidance on physical protection.

  8. Mersin ilinde görev yapan hekimlerin meslek örgütüyle ilişkileri ve beklentileri

    Directory of Open Access Journals (Sweden)

    Ahmet Öner Kurt

    2011-12-01

    Full Text Available Amaç: Bu çalışmada; Mersin İlinde görev yapan hekimlerin meslek örgütü olan tabip odası ile ilişkilerinin boyutu ve odadan beklentilerinin değerlendirilmesi amaçlanmıştır. Yöntem: Kesitsel tipteki bu çalışmada Mersin İlinde görev yapan toplam 1875 hekim evren olarak kabul edildi, örnek olarak 550 hekimin çalışmaya alınması hedeflendi. Çalışmaya alınacak hekimler; görev yeri, kurum, unvan ve cinsiyet oranına göre ağırlıklandırılarak tabakalı sistematik örnekleme yöntemi ile belirlendi. Veriler Kasım-Aralık 2009 tarihlerinde anket formu kullanılarak toplandı. Verilerin özetlenmesinde tanımlayıcı istatistikler ve değişkenlerin karşılaştırılmasında Ki-kare testi kullanıldı. Bulgular: Hekimlerin %69.1’i erkek, %82.5’i evli, %80.9’u kamu sağlık kurumlarında çalışıyor, %37.6’sı pratisyen hekim olarak görev yapıyordu. Hekimlerin ortalama görev süresi 17.4±9.6 (1–52 yıldı. Hekimlerin %77.1’i tabip odasına üye, %31.5’i son bir yılda tabip odası çalışmalarına katılmış ve %26.7’si bugünden sonraki süreçte tabip odası çalışmalarında görev almak istiyordu. Erkek, evli, özel sektörde çalışan, uzman olan, görev süresi 30 yıldan fazla olan ve çalışma koşullarının iyileştirilmesini bekleyen hekimlerin diğer gruplara göre tabip odasına daha fazla üye oldukları saptandı. Son bir yılda araştırma görevlileri tabip odası çalışmalarına daha az katılmıştı. Hekimlerin tabip odasından ilk sıradaki beklentisi; çalışma koşullarının iyileştirilmesiydi. Sonuç: Mersin’de dört hekimden birisinin tabip odasına üye olmaması, üçte ikisinin oda çalışmalarına katılmaması ve bugünden sonra da katılmayı düşünmemesi, hekimlerin örgütlü yaşamın gereklerini yeterince yerine getirmediğini ve meslek örgütlerine çok fazla sahip çıkmadığını düşündürmektedir. Tabip odaları üyelerinin odaya yakla

  9. EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2014. Scientific Opinion on Flavouring Group Evaluation 304, Revision 1 (FGE.304Rev1): Four carboxamides from Chemical Groups 30

    DEFF Research Database (Denmark)

    Beltoft, Vibe Meister; Binderup, Mona-Lise; Frandsen, Henrik Lauritz

    The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids of the European Food Safety Authority was requested to evaluate four flavouring substances in the Flavouring Group Evaluation 304, Revision 1 (FGE.304Rev1) using the Procedure in Commission Regulation (EC) No 1565...... criteria and identity for the materials of commerce have been provided for all four candidate substances....

  10. Evaluation of plasma sphingosine 1-phosphate, hepcidin and cardiovascular damage biomarkers (cardiac troponin I and homocysteine) in rats infected with brucellosis and vaccinated (Rev-1, RB-51).

    Science.gov (United States)

    Azimzadeh, Kaveh; Nasrollahi Nargesabad, Reza; Vousooghi, Nasim

    2017-08-01

    Brucellosis is known as one of important zoonosis. Studying the histological and biochemical effects of the disease could help to increase our knowledge about it. The aim of the present study was to evaluate changes of plasma parameters after intraperitoneal injection of two species of Brucella (Brucella melitensis and Brucella abortus) and two vaccines (Rev-1, RB-51) in the rat. Forty male rats were divided into five groups (n = 8 in each group). Two groups received suspensions of Brucella abortus and Brucella melitensis and two other groups were injected intraperitoneally with two mentioned vaccines and the last group received only distilled water. The results showed a significant increase in sphingosine 1-phosphate, Malondialdehyde, hepcidin, homocysteine, cardiac troponin I and copper levels and a considerable decrease in the levels of iron and zinc (P ≤ 0.01) in infected groups compared to the control animals. In vaccinated groups, hepcidin was increased but other parameters were not changed in comparison to the control group. It can be concluded that increase of homocysteine and cardiac troponin I in brucellosis could be a warning for cardiac adverse effects. Besides, increase of sphingosine 1-phosphate probably indicates its stimulant and modulatory effects in anti- Brucellosis biochemical pathways of the host. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. A Study on Improvement of Test Method of Nuclear Power Plant ESF ACS by applying Regulatory Guide 1.52 (Rev.3)

    International Nuclear Information System (INIS)

    Lee, Sook Kyung; Kim, Kwang Sin; Sohn, Soon Hwan; Song, Kyu Min; Lee, Kae Woo; Cho, Byoung Ho; Park, Jeong Seo; Yoo, Byeang Jea; Hong, Soon Joon; Kang, Sun Haeng

    2010-01-01

    U. S. NRC Regulation Guide 1.52 regulating ESF ACS in nuclear power plants has been revised to revision 3. To apply reduction of operability test time, allowance of alternative challenge agents for in-place leak test of HEPA filters, and upgrade of Methyl Iodide penetration acceptance criterion in activated carbon performance test suggested in Reg. Guide 1.52(Rev.3) on Yonggwang units 5 and 6 ESF ACSes, technical feasibility study was carried out with on-site experiments as well as experiments with a lab-scale model. It was confirmed that the moisture in the system returned to the level before the test in 1 or 4 days even though the moisture was removed during the operability test lasting more than 10 hours. Therefore, it is appropriate to perform monthly operability test in 15 minutes just long enough to check the operability of equipment. To change challenge material for in-place HEPA filter leak test, size of aerosol, production rate, and leak detection capability were compared for DOP and PAO. It was concluded that PAO can be substituted for DOP in nuclear power plants. The upgrade of Methyl Iodide penetration acceptance criterion from 0.175 % to 0.5 % in active carbon filter bed deeper than 4 inches was to conform to the change of activated carbon performance test method to ASTM D3803(1989). It was confirmed that Methyl Iodide penetration acceptance criterion of 0.5 % under 30 .deg. C

  12. Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the α-thyroid hormone receptor and Rev-erbα

    Directory of Open Access Journals (Sweden)

    Brown M Scott

    2010-12-01

    Full Text Available Abstract Background Alternative processing of α-thyroid hormone receptor (TRα, NR1A1 mRNAs gives rise to two functionally antagonistic nuclear receptors: TRα1, the α-type receptor, and TRα2, a non-hormone binding variant that is found only in mammals. TRα2 shares an unusual antisense coding overlap with mRNA for Rev-erbα (NR1D1, another nuclear receptor protein. In this study we examine the structure and expression of these genes in the gray short-tailed opossum, Monodelphis domestica, in comparison with that of eutherian mammals and three other marsupial species, Didelphis virginiana, Potorous tridactylus and Macropus eugenii, in order to understand the evolution and regulatory role of this antisense overlap. Results The sequence, expression and genomic organization of mRNAs encoding TRα1 and Rev-erbα are very similar in the opossum and eutherian mammals. However, the sequence corresponding to the TRα2 coding region appears truncated by almost 100 amino acids. While expression of TRα1 and Rev-erbα was readily detected in all tissues of M. domestica ages 0 days to 18 weeks, TRα2 mRNA was not detected in any tissue or stage examined. These results contrast with the widespread and abundant expression of TRα2 in rodents and other eutherian mammals. To examine requirements for alternative splicing of TRα mRNAs, a series of chimeric minigenes was constructed. Results show that the opossum TRα2-specific 5' splice site sequence is fully competent for splicing but the sequence homologous to the TRα2 3' splice site is not, even though the marsupial sequences are remarkably similar to core splice site elements in rat. Conclusions Our results strongly suggest that the variant nuclear receptor isoform, TRα2, is not expressed in marsupials and that the antisense overlap between TRα and Rev-erbα thus is unique to eutherian mammals. Further investigation of the TRα and Rev-erbα genes in marsupial and eutherian species promises to yield

  13. Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the α-thyroid hormone receptor and Rev-erbα

    Science.gov (United States)

    2010-01-01

    Background Alternative processing of α-thyroid hormone receptor (TRα, NR1A1) mRNAs gives rise to two functionally antagonistic nuclear receptors: TRα1, the α-type receptor, and TRα2, a non-hormone binding variant that is found only in mammals. TRα2 shares an unusual antisense coding overlap with mRNA for Rev-erbα (NR1D1), another nuclear receptor protein. In this study we examine the structure and expression of these genes in the gray short-tailed opossum, Monodelphis domestica, in comparison with that of eutherian mammals and three other marsupial species, Didelphis virginiana, Potorous tridactylus and Macropus eugenii, in order to understand the evolution and regulatory role of this antisense overlap. Results The sequence, expression and genomic organization of mRNAs encoding TRα1 and Rev-erbα are very similar in the opossum and eutherian mammals. However, the sequence corresponding to the TRα2 coding region appears truncated by almost 100 amino acids. While expression of TRα1 and Rev-erbα was readily detected in all tissues of M. domestica ages 0 days to 18 weeks, TRα2 mRNA was not detected in any tissue or stage examined. These results contrast with the widespread and abundant expression of TRα2 in rodents and other eutherian mammals. To examine requirements for alternative splicing of TRα mRNAs, a series of chimeric minigenes was constructed. Results show that the opossum TRα2-specific 5' splice site sequence is fully competent for splicing but the sequence homologous to the TRα2 3' splice site is not, even though the marsupial sequences are remarkably similar to core splice site elements in rat. Conclusions Our results strongly suggest that the variant nuclear receptor isoform, TRα2, is not expressed in marsupials and that the antisense overlap between TRα and Rev-erbα thus is unique to eutherian mammals. Further investigation of the TRα and Rev-erbα genes in marsupial and eutherian species promises to yield additional insight into the

  14. Quantum theory. 2. rev. ed.

    International Nuclear Information System (INIS)

    Mitter, H.

    1979-01-01

    This book is an introduction into quantum mechanics. After a general introduction the algebraic calculus of quantum mechanics in the framework of the Dirac brackets is described, whereby the probabilistic interpretation is introduced. Then some simple examples are described in this framework. After a description of the wave representation the general formalism of quantum mechanics is described. Then the Schroedinger equation is introduced. The angular momentum in quantum mechanics is then explicitely discussed. After a treatment of simple one- and two-body problems the parity and the probability current are discussed. Then the approximation methods are described. Finally some applications in atomic physics, simples many-body problems, and the scattering theory are dealed with. In the appendix the delta-function, orthogonal functions, the higher symmetry of the hydrogen problem, and the Galilei transformation in quantum mechanics are described. Every chapter conteins exercise problems. (HSI) [de

  15. Nuclear Propulsion for Space (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Corliss, William R; Schwenk, Francis C

    1971-01-01

    The operation of nuclear rockets and a description of the development of nuclear rockets in the U.S. is given. Early developments and Project Rover, Project Pluto, and the NERVA (Nuclear Engine for Rocket Vehicle Application) Program are detailed. The Nuclear Rocket Development Station facilities in Nevada are described. The possibilities and advantages of using nuclear rockets for missions beginning from an earth orbit and moving outward toward higher earth orbits, the moon, and the planets are discussed.

  16. Food Preservation by Irradiation (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Urrows, Grace M.

    1968-01-01

    Up to 30% of food harvests are lost in some parts of the world because of animal pests and microorganisms. Nuclear techniques can help reduce and extend the shelf life of these foods. Around 55 countries now have food irradiation programs. The use of radiation is the most recent step in man's attempts to preserve some of his harvest for the lean part of the year.

  17. Mineralogy. 2. rev., enlarged ed.

    International Nuclear Information System (INIS)

    Matthes, S.

    1987-01-01

    The textbook is intended for students with a basic knowledge of descriptive mineralogy and crystallography who wish to enhance their knowledge in the fields of determinative mineralogy, petrology, and the geology and genesis of mineral deposits. The material presented concentrates on the essential aspects of the subject fields and is completed by up-to-date information concerning the technical and economic significance of the minerals, rocks and ores as raw materials. With 165 figs., 2 tabs [de

  18. Project Execution Plan, Rev. 3

    International Nuclear Information System (INIS)

    IT Corporation, Las Vegas

    2002-01-01

    This plan addresses project activities encompassed by the U.S. Department of Energy's (DOE's), National Nuclear Security Administration Nevada Operations Office, Environmental Restoration Division and conforms to the requirements contained in the Life-Cycle Asset Management, DOE Order 430.1A; The Joint Program Office Policy on Project Management in Support of DOE Order 430.1; Program and Project Management for the Acquisition of Capital Assets, DOE Order 413.3; the Project Execution and Engineering Management Planning Guide, GPG-FM-010; and other applicable Good Practice Guides; and the FY 2001 Integrated Planning, Accountability, and Budgeting System Policy Guidance. The plan also reflects the milestone philosophies of the Federal Facility Agreement and Consent Order, as agreed to by the State of Nevada, the DOE, and the U.S. Department of Defense; and traditional project management philosophies such as the development of life-cycle costs, schedules, and work scope; identification o f roles and responsibilities; and baseline management and controls

  19. Subconsciously opposed. 2. rev. ed.

    International Nuclear Information System (INIS)

    Wuenschmann, A.

    1982-01-01

    The book deals with the emotional aspects of the controversy on peaceful use of nucleon energy. It would be self-delusion to expect a near end of the nuclear discussion. Advocates and opponents confront each other almost beyond reconcilation, handly prepared to hear the opposite side's arguments. What is the reason. As C.F. von Weizsaecker remarked to the author: 'To understand a phenomenon like the present nuclear energy debate is hardly possible without recurring to psychology'. In this paperback an entirely new approach to the nuclear energy debate is opened. The book has found wide attention. As the 'Neue Zuercher Zeitung' wrote: 'The author's psychology analysis of the nuclear energy controversy and especially its interpretation based on Jung's depth psychology combine to form a terse survey of many phenomena not so far systematically studied'. (orig.) [de

  20. The nuclear suicide. Rev. ed.

    International Nuclear Information System (INIS)

    Gruhl, H.

    1988-01-01

    The author, one of the most consistent opponents of nuclear power, strikes a balance after the Chernobyl catastrophe. He relentlessly analyses the 'German nuclear situation', developments to be expected from risks and catastrophic events. He exposes statements offered by the owners of power plants and by politicians as attempts to play risks down, and hopes to achieve a turnabout yet. The conjunction of technical and physical arguments with a fundamental criticism of the ideology of economic growth is what distinguishes this book from other publications critical of nuclear energy. But the author does not content himself with criticism, he goes on to point out realistic measures for opting out of nuclear energy. (orig./HSCH) With 10 figs. and maps [de

  1. Reactor handbook. 2. rev. ed.

    International Nuclear Information System (INIS)

    Lederer, B.J.; Wildberg, D.W.

    1992-01-01

    On the basis of the guidelines on expert knowledge, the book discusses the subjects of atomic physics, heat transfer, nuclear power plants, reactor materials, radiation protection, reactor safety, reactor instrumentation, and reactor operation, with special regard to nuclear power plants with LWR-type reactors. The book is intended for shift personnel, especially gang bosses, reactor operators, and control station operators: for this reason a practical and rather popular style has been chosen. However, the book will also be a manual for other operating personnel, personnel of producer companies, expert organisations, authorities, and students. It can be used as a textbook for staff training, a manual for the practice, and as accompanying book for teaching at nuclear engineering schools. (orig.) With 173 figs [de

  2. Animals in Atomic Research (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Ricciuti, Edward R. [Bronx Zoo

    1969-01-01

    This booklet explains what use animals are to science and why they are important to the development of nuclear energy for peaceful uses. It contains examples of the roles animals of many kinds play in the development of nuclear science for the well-being of mankind.

  3. 15 years of Rev Rene

    Directory of Open Access Journals (Sweden)

    Lorita Marlena Freitag Pagliuca

    2015-04-01

    Full Text Available Writing an editorial is always a challenge. When drafting a scientific paper one can rely on the literature review, method, results, and discussion with texts supporting or deconstructing what one wants to prove. Utopia is the spice of life, better when there are more than one utopian; particularly a group of dreamers who allowed themselves to dream, not worrying about immediate results; keen to build and preserve a 15-year history. Enter adolescence and be in the world. Create complicity to dream and achieve utopia.

  4. Environmental law. 3. rev. ed.

    International Nuclear Information System (INIS)

    Anon.

    1985-01-01

    This pocketbook contains major federal regulations on environmental protection. They serve to protect and cultivate mankind's natural foundations of life, to preserve the environment. The environmental law is devided as follows: Constitutional law on the environment, common administrative law on the environment, special administrative law on the environment including conservation of nature and preservation of rural amenities, protection of waters, waste management, protection against nuisances, nuclear energy and radiation protection, energy conservation, protection against dangerous substances, private law relating to the environment, criminal law relating to the environment. (orig.) [de

  5. Nuclear Energy for Desalting (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Urrows, Grace M.

    1967-01-01

    This booklet discusses the huge demand for fresh clean water and various ways to create it. Since 3/4 of the world is covered in salt water, many processes have been developed to desalt the salt water. Nuclear-powered desalting units are discussed extensively.

  6. Eddy Current Testing at Level 2: Manual for the Syllabi Contained in IAEA-TECDOC-628.Rev. 2 'Training Guidelines for Non Destructive Testing Techniques'

    International Nuclear Information System (INIS)

    2011-01-01

    The International Atomic Energy Agency has been active in the promotion of non-destructive testing (NDT) technology in the world for many decades. The prime reason for this interest has been the need for stringent standards for quality control for safe operation of nuclear as well as other industrial installations. It has successfully executed a number of programmes including technical co-operation (TC) projects (national and regional) and the coordinated research projects (CRP) of which NDT was an important part. Through these programmes a large number of persons in the Member States have been trained, leading to establishment of national certifying bodies (NCB) responsible for training and certification of NDT personnel. Consequently, a state of self-sufficiency in this area of technology has been achieved in many of them. All along there has been a realization of the need to have well established training guidelines and related books in order, firstly, to guide the IAEA experts who were involved in this training programme and, secondly, to achieve some level of international uniformity and harmonization of training materials and consequent competence of NDT personnel. The syllabi for training courses have been published in the form of IAEA-TECDOC publications. The first was IAEA-TECDOC-407 (1987), which contained syllabi for the basic five methods, i.e. liquid penetrant testing, magnetic particle testing, eddy current testing, radiographic testing and ultrasonic testing. To accommodate advancements in NDT technology, later versions of this publication were issued in 1991, 2002 and 2008, the current version being IAEA-TECDOC-628/Rev.2 (2008), which includes additional and more advanced NDT methods. This IAEA-TECDOC, as well as most of the international standards on the subject of training and certification of NDT personnel including ISO 9712 (2005), define three levels of competence. Among these, level 1 is the lowest and level 3 the highest. The intermediate

  7. Development of a Computer Code, PZRTR rev 1, for the Thermal Hydraulic Analysis of a Multi-Cavity Cold Gas Pressurizer for an Integral Reactor, SMART-P

    Energy Technology Data Exchange (ETDEWEB)

    Seo, Jae Kwang; Kang, H. O.; Yoon, J.; Kim, K. K

    2006-12-15

    The concept of a Multi-cavity Cold Gas PressuriZeR(MCGPZR) is applied to the SMART: The pressurizer system includes in-vessel cavities and out-of-vessel gas cylinders holding the gas supply/vent system. The gas cylinders are connected to the one of the in-vessel cavities via piping with valves. A pressurizer is maintained at a cold temperature of less than about 120 .deg. C which is realized with coolers installed in and with wet thermal insulators installed on one of the cavities located inside the hot reactor vessel, to minimize the contribution of a steam partial pressure and is filled with nitrogen gas as a pressure-absorbing medium. The working medium and working temperature of the MCGPZR is totally different from that of a hot steam pressurizer of the commercial PWR. In addition, the MCGPZR is intended to be designed to meet a pressure transient during normal power operation (by its gas volume capacity) without using an active control system and during plant heatup/cooldown operation by using an active gas control (filling/venting) system. Therefore in order to evaluate the feasibility of the concept of the MCGPZR and its intended design goal, the thermal hydraulic behaviors and controllability of the MCGPZR during transients especially a heatup/cooldown operation must be analyzed. In this study, a thermal hydraulic transient analysis computer code, PZRTR rev 1, for the Reactor Coolant System(RCS) of an integral reactor composed of the MCGPZR, modular Once-Through Steam Generators(OTSGs), a core and a reactor coolant loop is developed. The pressurizer module (MCGPZR module) of the PZRTR rev 1 code is based on a two-fluid, nonhomogeneous, nonequilibrium model for the two-phase system behavior and the OTSG module is based on a homogeneous equilibrium model of the two-phase flow process. The core module is simply based on the axial power distributions and the reactor coolant loop is based on the temperature distributions. The code is currently dedicated for the

  8. Visual Acuity Change over 12 Months in the Prospective Progression of Atrophy Secondary to Stargardt Disease (ProgStar) Study: ProgStar Report Number 6.

    Science.gov (United States)

    Kong, Xiangrong; Strauss, Rupert W; Cideciyan, Artur V; Michaelides, Michel; Sahel, José-Alain; Munoz, Beatriz; Ahmed, Mohamed; Ervin, Ann M; West, Sheila K; Cheetham, Janet K; Scholl, Hendrik P N

    2017-11-01

    To estimate the yearly rate of change of best-corrected visual acuity (BCVA) and the risk of loss 1 line or more over 1 year and to identify risk factors for BCVA loss in patients with Stargardt disease (STGD1). Multicenter, prospective cohort study. Two hundred fifty-nine patients (489 eyes) with molecularly confirmed STGD1 enrolled at 9 centers in the United States and Europe. Participants were followed up every 6 months, and data at the baseline and 6- and 12-month visits were analyzed. Best-corrected visual acuity was measured using the Early Treatment Diabetic Retinopathy Study (ETDRS) protocol. Standardized reporting forms were used to collect participants' characteristics and clinical observations. Linear mixed effects models were used to estimate the rate of BCVA loss. Linear models with generalized estimating equations were used to identify risk factors for BCVA loss of 1 line or more over 1 year. Change in BCVA over 1 year. Cross-sectional analysis at baseline showed that earlier symptom onset and longer duration since onset was associated with worse BCVA. Longitudinal analysis showed no overall significant change of BCVA within 12 months, but the rate of BCVA change was significantly different by baseline BCVA (P trials with 1 year's duration. Copyright © 2017 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

  9. Limit Stress Spline Models for GRP Composites | Ihueze | Nigerian ...

    African Journals Online (AJOL)

    Spline functions were established on the assumption of three intervals and fitting of quadratic and cubic splines to critical stress-strain responses data. Quadratic ... of data points. Spline model is therefore recommended as it evaluates the function at subintervals, eliminating the error associated with wide range interpolation.

  10. Detection of osmotic damages in GRP boat hulls

    Science.gov (United States)

    Krstulović-Opara, L.; Domazet, Ž.; Garafulić, E.

    2013-09-01

    Infrared thermography as a tool of non-destructive testing is method enabling visualization and estimation of structural anomalies and differences in structure's topography. In presented paper problem of osmotic damage in submerged glass reinforced polymer structures is addressed. The osmotic damage can be detected by a simple humidity gauging, but for proper evaluation and estimation testing methods are restricted and hardly applicable. In this paper it is demonstrated that infrared thermography, based on estimation of heat wave propagation, can be used. Three methods are addressed; Pulsed thermography, Fast Fourier Transform and Continuous Morlet Wavelet. An additional image processing based on gradient approach is applied on all addressed methods. It is shown that the Continuous Morlet Wavelet is the most appropriate method for detection of osmotic damage.

  11. ποταμοφόρητος (Rev 12 : 15 – from Etymology to Theologoumenon

    Directory of Open Access Journals (Sweden)

    Wiesław Alicki

    2013-06-01

    Full Text Available The paper presents a study on translations of a Greek word ποταμοφόρητος occurring in Rev 12 : 15. The Greek hapax legomenon ποταμοφόρητος is used in Rev 12 : 15. The part of the sentence containing ποταμοφόρητος is translated as: to sweep her away with the current. G. Schneider suggests a correction of the meaning of the word, concentrating on the conjectural purpose of being taken by water (drowning. In order to analyse this issue we begin with the study of etymology of the ποταμοφόρητος. It comes from the noun ποταμός and the adjective φορητός, which is a derivative of φορέω. Composita (compounds formed with φορητός did not refer to any idea of a mortal threat. Till the turn of 19th century ποταμοφόρητος, apart from the Bible, was only certified in the Hesychius’ Lexicon (5th or 6th century. The Egyptian papyri, uncovered and analysed on the turn of the 19th and the 20th century, in which the ποταμοφόρητος is related to the overflows of the Nile, let us doubt Schneider’s proposal, however, still do not solve the problem. The next stage of the research consisted of the analysis of the classical and antique translations of Rev (VL, Vg, Syriac and Coptic translations, as well as Latin and Greek patristic commentaries, which support one of the aforementioned interpretations. The commentators understood the river carrying the Woman as the image of Church persecution and a desire to place the Church under the command of evil forces. One of the versions of the translation of Vetus Latina (type I suggests that the archetype of the water torrent in Rev was the Nile. In the light of classical explanations as to the reasons of the river’s floodings, as well as the parallel in Ezek 29 : 3. 6 we can assume that it was the Nile that served as the archetype of the spewing torrent for the author of the Revelation.

  12. Translesion Synthesis of the N2-2′-Deoxyguanosine Adduct of the Dietary Mutagen IQ in Human Cells: Error-Free Replication by DNA Polymerase κ and Mutagenic Bypass by DNA Polymerases η, ζ, and Rev1

    Science.gov (United States)

    2016-01-01

    Translesion synthesis (TLS) of the N2-2′-deoxyguanosine (dG-N2-IQ) adduct of the carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) was investigated in human embryonic kidney 293T cells by replicating plasmid constructs in which the adduct was individually placed at each guanine (G1, G2, or G3) of the NarI sequence (5′-CG1G2CG3CC-3′). TLS efficiency was 38%, 29%, and 25% for the dG-N2-IQ located at G1, G2, and G3, respectively, which suggests that dG-N2-IQ is bypassed more efficiently by one or more DNA polymerases at G1 than at either G2 or G3. TLS efficiency was decreased 8–35% in cells with knockdown of pol η, pol κ, pol ι, pol ζ, or Rev1. Up to 75% reduction in TLS occurred when pol η, pol ζ, and Rev1 were simultaneously knocked down, suggesting that these three polymerases play important roles in dG-N2-IQ bypass. Mutation frequencies (MFs) of dG-N2-IQ at G1, G2, and G3 were 23%, 17%, and 11%, respectively, exhibiting a completely reverse trend of the previously reported MF of the C8-dG adduct of IQ (dG-C8-IQ), which is most mutagenic at G3 ((2015) Nucleic Acids Res.43, 8340−835126220181). The major type of mutation induced by dG-N2-IQ was targeted G → T, as was reported for dG-C8-IQ. In each site, knockdown of pol κ resulted in an increase in MF, whereas MF was reduced when pol η, pol ι, pol ζ, or Rev1 was knocked down. The reduction in MF was most pronounced when pol η, pol ζ, and Rev1 were simultaneously knocked down and especially when the adduct was located at G3, where MF was reduced by 90%. We conclude that pol κ predominantly performs error-free TLS of the dG-N2-IQ adduct, whereas pols η, pol ζ, and Rev1 cooperatively carry out the error-prone TLS. However, in vitro experiments using yeast pol ζ and κ showed that the former was inefficient in full-length primer extension on dG-N2-IQ templates, whereas the latter was efficient in both error-free and error-prone extensions. We believe that the observed differences between

  13. pjm1 prog.html | myrmeet | meetings | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    2018-03-13

    Lists of selected candidates. Interaction Meeting on Women In Science. Posted on 12 March 2018. Meeting will be on March 13, 2018 in Multimedia Room, Physics Department, IISc. 29th Mid-year meeting. Posted on 19 January 2018. The 29th Mid-year meeting of the Academy will be held from 29–30 June 2018 in Infosys, ...

  14. Brane-world cosmology and inflation

    Indian Academy of Sciences (India)

    A Lukas and D Skinner, J. High Energy Phys. 0109, 020 (2001). M C Bento, O Bertolami and A A Sen, Phys. Rev. D67, 063511 (2003). [8] J Garriga and M Sasaki, Phys. Rev. D62, 043523 (2000). [9] K Koyama and J Soda, Phys. Lett. B483, 432 (2000). [10] S Kanno, M Sasaki and J Soda, Prog. Theor. Phys. 109, 357 (2003).

  15. The uvsI gene of Aspergillus nidulans required for UV-mutagenesis encodes a homolog to REV3, a subunit of the DNA polymerase zeta of yeast involved in translesion DNA synthesis.

    Science.gov (United States)

    Han, K Y; Chae, S K; Han, D M

    1998-07-01

    Defects in the uvsI gene of Aspergillus nidulans resulted in high UV sensitivity and reductions of spontaneous and UV-induced reversion of certain alleles, uvsl;uvsA double mutants exhibited high methyl methane sulfonate (MMS)-sensitivity in contrast to the slight sensitivity of the component single mutants. Using such a double mutant as recipient, a clone complementing uvsI501 has been isolated from a chromosome III specific library. The deduced amino acid sequence from the 1.1-kb sequenced region, a part of the 5.2-kb DNA fragment showing uvsI-complementing activity, had a 62% identity with REV3 of yeast. Disruptants of the cloned gene demonstrated the same level of sensitivity to UV light as uvsI and failed to complement uvsI501 in heterozygous diploids.

  16. Pixar y la recepción de sus valores morales en el público infantil : aproximación a la investigación de la audiencia a partir del caso "Del revés"

    OpenAIRE

    Gutiérrez Cardona, Sònia

    2016-01-01

    Aquest treball té l'objectiu d'analitzar la validesa educativa del plantejament de la filmografia de Pixar. El projecte també pretén esbrinar si aquest rigor educatiu s'adapta als espectadors infantils de Pixar. L'objecte d'estudi de la investigació és la recepció de valors en les pel·lícules de la productora cinematogràfica a partir del cas "Del revés". L'anàlisi de valors s'ha realitzat a una mostra de públic infantil de 45 subjectes, aplicant un Test de presència de valors, ideat i creat p...

  17. Reclassification of Flexibacter tractuosus (Lewin 1969) Leadbetter 1974 and 'Microscilla sericea' Lewin 1969 in the genus Marivirga gen. nov. as Marivirga tractuosa comb. nov. and Marivirga sericea nom. rev., comb. nov.

    Science.gov (United States)

    Nedashkovskaya, Olga I; Vancanneyt, Marc; Kim, Seung Bum; Bae, Kyung Sook

    2010-08-01

    The taxonomic position of the misclassified strains [Flexibacter] tractuosus KCTC 2958T and '[Microscilla] sericea' LMG 13021 was studied using a polyphasic approach. The two strains shared 99.1% 16S rRNA gene sequence similarity and 28% DNA-DNA relatedness. On the basis of the phylogenetic evidence supported by genotypic and phenotypic data [Flexibacter] tractuosus KCTC 2958T and '[Microscilla] sericea' LMG 13021 are classified as two distinct species in a novel genus, Marivirga, in the family 'Flammeovirgaceae', as Marivirga tractuosa comb. nov. and Marivirga sericea nom. rev., comb. nov., with strains KCTC 2958T (=ATCC 23168T =CIP 106410T =DSM 4126T =NBRC 15989T =NCIMB 1408T =VKM B-1430T) and LMG 13021T (=ATCC 23182T =NBRC 15983T =NCIMB 1403T), respectively, as the type strains. The type species is Marivirga tractuosa.

  18. PERCEPTIONS OF ORGANIZATIONAL JUSTICE WHICH TEACHERS EMPLOYED IN PRIVATE TEACHING CENTER ÖZEL DERSHANELERDE GÖREV YAPAN ÖĞRETMENLERİN ÖRGÜTSEL ADALET ALGILARI

    Directory of Open Access Journals (Sweden)

    Mustafa YAVUZ

    2012-06-01

    Full Text Available The purpose of this study is to determine the variables that predict perceptions of organizational justice which teachers employed in private teaching center (private institutions preparing students for university entrance examinations have and identify the relationship between perceptions of organizational justice which employees have and their demographic characteristics. The study is of general survey model. The population of the study consists of teachers employed in private teaching center in the Central Anatolia Region in 2008-2009 academic year. The study sample, on the other hand, includes 404 randomly selected teachers who are employed in private teaching center in the provinces of Ankara, Konya, Aksaray, Kayseri and Nevşehir. The research findingsindicate that variables of social security and job security are predictors of employees’ perceptions of organizational justice. Bu araştırmanın amacı, özel dershanelerde görev yapan öğretmenlerin örgütsel adalet algılamalarını yordayan değişkenlerin tespit edilmesi ve çalışanların örgütsel adalet algılamaları ile demografik özellikleri arasındaki ilişkiyi belirlemektir. Araştırma genel tarama modelindedir. Araştırmanın evrenini İç Anadolu bölgesindeki dershanelerde çalışan öğretmenler oluşturmaktadır. Araştırmanın örneklemi ise; tesadüfi örnekleme yöntemiyle seçilen Ankara, Konya, Aksaray, Kayseri ve Nevşehir illerindeki dershanelerde görev yapan 404 öğretmenden oluşmaktadır. Araştırmanın bulguları, sosyal güvenlik ve iş güvencesi değişkenlerinin çalışanların örgütsel adalet algılamalarının yordayıcısı olduğunu göstermektedir.

  19. EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (CEF); Scientific Opinion on Flavouring Group Evaluation 11, Revision 2 (FGE.11Rev2): Aliphatic dialcohols, diketones, and hydroxyketones from chemical groups 8 and 10

    DEFF Research Database (Denmark)

    Larsen, John Christian; Nørby, Karin Kristiane; Beltoft, Vibe Meister

    The Scientific Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (the Panel) was asked to provide scientific advice to the Commission on the implications for human health of chemically defined flavouring substances used in or on foodstuffs in the Member States. In particular......, the Panel was requested to evaluate 12 flavouring substances in the Flavouring Group Evaluation 11, Revision 2 (FGE.11Rev2), using the Procedure as referred to in the Commission Regulation (EC) No 1565/2000. These 12 flavouring substances belong to chemical group 10, Annex I of the Commission Regulation (EC...... is a tertiary alcohol) [FL-no: 07.097, 07.165 and 07.184] all belonging to chemical groups 8 and 10. One of the 12 candidate substances possesses four chiral centres [FL-no: 06.134] two possesses two chiral centres [FL-no: 02.133 and 07.168] and four substances possesses one chiral centre [FL-no: 07.097, 07...

  20. The Rev1 interacting region (RIR) motif in the scaffold protein XRCC1 mediates a low-affinity interaction with polynucleotide kinase/phosphatase (PNKP) during DNA single-strand break repair.

    Science.gov (United States)

    Breslin, Claire; Mani, Rajam S; Fanta, Mesfin; Hoch, Nicolas; Weinfeld, Michael; Caldecott, Keith W

    2017-09-29

    The scaffold protein X-ray repair cross-complementing 1 (XRCC1) interacts with multiple enzymes involved in DNA base excision repair and single-strand break repair (SSBR) and is important for genetic integrity and normal neurological function. One of the most important interactions of XRCC1 is that with polynucleotide kinase/phosphatase (PNKP), a dual-function DNA kinase/phosphatase that processes damaged DNA termini and that, if mutated, results in ataxia with oculomotor apraxia 4 (AOA4) and microcephaly with early-onset seizures and developmental delay (MCSZ). XRCC1 and PNKP interact via a high-affinity phosphorylation-dependent interaction site in XRCC1 and a forkhead-associated domain in PNKP. Here, we identified using biochemical and biophysical approaches a second PNKP interaction site in XRCC1 that binds PNKP with lower affinity and independently of XRCC1 phosphorylation. However, this interaction nevertheless stimulated PNKP activity and promoted SSBR and cell survival. The low-affinity interaction site required the highly conserved Rev1-interacting region (RIR) motif in XRCC1 and included three critical and evolutionarily invariant phenylalanine residues. We propose a bipartite interaction model in which the previously identified high-affinity interaction acts as a molecular tether, holding XRCC1 and PNKP together and thereby promoting the low-affinity interaction identified here, which then stimulates PNKP directly. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  1. Energy from agricultural animal wastes. Rev. ed.

    International Nuclear Information System (INIS)

    1994-12-01

    This technology status report discusses the production of animal wastes in the UK, and the environmental problems caused by direct use of the waste as fertilisers or disposal by landfilling. The effects of the moisture content of the waste on the choice of technology is examined, and the use of anaerobic digestion for wetter wastes to produce biogas and combustion of dryer wastes to produce heat, power and phosphate- and potash-rich ash are discussed. Market opportunities, and the economics and environmental impacts of both technologies are investigated

  2. ENDF/B-5 Actinides (Rev. 86)

    International Nuclear Information System (INIS)

    Lemmel, H.D.

    1986-05-01

    This document summarizes the contents of the Actinides part of the ENDF/B-5 nuclear data library released by the US National Nuclear Data Center. This library or selective retrievals of it, are available costfree from the IAEA Nuclear Data Section upon request. The present version of the library is the Revision of 1986. (author). Refs, figs and tabs

  3. Facts on nuclear power. 4. rev. ed.

    International Nuclear Information System (INIS)

    1987-06-01

    The information booklet summarizes physical principles and other fundamental background information relating to nuclear power. The material is subdivided into various brief chapters with tables and illustrations discussing aspects such as: Basic terms and data, energy demand and supply, economics, nuclear power plants in operation, radiation protection, reactor safety, and nuclear waste management. A brief outline of the history of nuclear power in the FRG is given, and a list of addresses and literature for further reading. (UA) [de

  4. Instructor Revs Up Ailing Motorcycle Program

    Science.gov (United States)

    Stevenson, Roy

    2011-01-01

    Bob Monroig smiles every day on his way to work at Lake Washington Technical College (LWTC), high atop a hill overlooking the affluent Seattle suburb of Kirkland, Washington. He's created his own job within a job, where he's the Harley-Davidson University program coordinator and tenured faculty in the Motorcycle, Marine, & Power Equipment…

  5. The First Reactor, 40th Anniversary (rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Allardice, Corbin; Trapnell, Edward R; Fermi, Enrico; Fermi, Laura; Williams, Robert C

    1982-12-01

    This booklet, an updated version of the original booklet describing the first nuclear reactor, was written in honor of the 40th anniversary of the first reactor or "pile". It is based on firsthand accounts told to Corbin Allardice and Edward R. Trapnell, and includes recollections of Enrico and Laura Fermi.

  6. Transesophageal echocardiography. 3. rev. and enl. ed.

    International Nuclear Information System (INIS)

    Lambertz, Heinz; Lethen, Harald

    2013-01-01

    The book on transesophageal echocardiography covers the following issues: Development of transesophageal echocardiography, technical advances; indications and contraindication for transesophageal echocardiography; systematic of the medical examination process; cardiac valves and valve prostheses; mitral and aortic valvuloplasty, TAVI and interventional treatment of mitral regurgitation; infectious endocarditis; one-way and effluence disturbances of the left and right ventricle; diseases of the thoracic aorta; undefined right ventricle enlargement; lung embolism, acute infarct complications; TEE during anesthesia and perioperative intensive medicine, cardiac sources of embolism; cardiac tumors, mediastinal lymph nodes; pericardiac diseases; congenital heart diseases in childhood and adulthood; catheter interventions and heart valve reconstruction; surgically corrected congenital cardiac defects; intracavitary versus transesophageal echocardiography; three-dimensional TEE; coronary diagnostics; ischemia and vitality diagnostics.

  7. Imaging pelvic floor disorders. 2. rev. ed.

    International Nuclear Information System (INIS)

    Stoker, Jaap; Taylor, Stuart A.; DeLancey, John O.L.

    2008-01-01

    This volume builds on the success of the first edition of imaging pelvic floor disorders and is aimed at those practitioners with an interest in the imaging, diagnosis and treatment of pelvic floor dysfunction. Concise textual information from acknowledged experts is complemented by high-quality diagrams and images to provide a thorough update of this rapidly evolving field. Introductory chapters fully elucidate the anatomical basis underlying disorders of the pelvic floor. State of the art imaging techniques and their application in pelvic floor dysfunction are then discussed in detail. Additions since the first edition include consideration of the effect of aging and new chapters on perineal ultrasound, functional MRI and MRI of the levator muscles. The closing sections of the book describe the modern clinical management of pelvic floor dysfunction, including prolapse, urinary and faecal incontinence and constipation, with specific emphasis on the integration of diagnostic and treatment algorithms. (orig.)

  8. Detectors for particle radiation. 2. rev. ed.

    International Nuclear Information System (INIS)

    Kleinknecht, K.

    1987-01-01

    This book is a description of the set-up and mode of action of detectors for charged particles and gamma radiation for students of physics, as well as for experimental physicists and engineers in research and industry: Ionization chamber, proportional counter, semiconductor counter; proportional chamber, drift chamber, bubble chamber, spark chamber, photomultiplier, laser ionization, silicion strip detector; Cherenkov counter, transition radiation detector; electron-photon-cascade counter, hadron calorimeter; magnetic spectrometer; applications in nuclear medicine, geophysics, space travel, atom physics, nuclear physics, and high-energy physics. With 149 figs., 20 tabs [de

  9. Occupational safety manual. 4. rev. ed.

    International Nuclear Information System (INIS)

    Skiba, R.

    1979-01-01

    With the rapid technical development and increasing life expectancy, protection against occupational hazards has become a central economic and social problem. Consultants and others responsible for occupational safety should not only be interested in their work but also have sufficient basic knowledge. The complex subject is presented in a simplified and easily comprehensible manner, with particular regard to the practical side. The book is at the same time a textbook for basic training and a reference manual for the working practice. (orig./HP) [de

  10. Environmental economics. 4. rev. and enl. ed.

    International Nuclear Information System (INIS)

    Wicke, L.

    1993-01-01

    This textbook is intended to reveal and explain the most important issues in environmental economics, a) on the basis of a systematic breakdown of the whole field of environmental economics, and b) with the aid of numerous examples taken from practical environmental policy. The material presented in this book has been grouped according to various subjects as, e.g., instruments of environmental policy, effects of pollution control measures on the economy, etc. The conclusions drawn by the author as a result of his study can be summarized in the following general statement: Democratic states with a free economic system corresponding to the social market economy of the FRG for instance are well endowed with the prerequisites for successfully tackling environmental problems, both in terms of financial and regulatory policy- and experience has already shown that the approach adopted is a practicable and promising one. However, there could be much more success along this line of the political instruments were given greater impetas and impact, and if the regulatory instruments were designed so as to gain more effectiveness. (orig.) [de

  11. Thermodynamics and statistical physics. 2. rev. ed.

    International Nuclear Information System (INIS)

    Schnakenberg, J.

    2002-01-01

    This textbook covers tthe following topics: Thermodynamic systems and equilibrium, irreversible thermodynamics, thermodynamic potentials, stability, thermodynamic processes, ideal systems, real gases and phase transformations, magnetic systems and Landau model, low temperature thermodynamics, canonical ensembles, statistical theory, quantum statistics, fermions and bosons, kinetic theory, Bose-Einstein condensation, photon gas

  12. Radiology of the pancreas. 2. rev. ed.

    International Nuclear Information System (INIS)

    Baert, A.L.; Van Hoe, L.; Delorme, G.

    1999-01-01

    Radiology of the Pancreas discusses the diagnostic role of the various imaging modalities currently available for the assessment of pancreatic anatomy and disease. In comparison with the first edition, new technical developments (helical CT, ultrafast magnetic resonance imaging, color Doppler ultrasound, laparoscopic ultrasound), have been included, and several chapters have been significantly expanded. With the aid of numerous illustrations, the normal radiological anatomy, anatomical variants, the typical and atypical radiological features of both common and uncommon diseases, and potential pitfalls are considered in depth. All of the chapters have been written by recognized experts in the field, and the book should be of value to all radiologists and other specialists who treat patients with pancreatic disease or who have an interest in the subject. (orig.)

  13. Nuclear Security: Target Analysis-rev

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Surinder Paul [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Gibbs, Philip W. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bultz, Garl A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2014-03-01

    The objectives of this presentation are to understand target identification, including roll-up and protracted theft; evaluate target identification in the SNRI; recognize the target characteristics and consequence levels; and understand graded safeguards.

  14. Project Execution Plan,Rev. 3; FINAL

    International Nuclear Information System (INIS)

    IT Corporation, Las Vegas

    2002-01-01

    This plan addresses project activities encompassed by the U.S. Department of Energy's (DOE's), National Nuclear Security Administration Nevada Operations Office, Environmental Restoration Division and conforms to the requirements contained in the Life-Cycle Asset Management, DOE Order 430.1A; The Joint Program Office Policy on Project Management in Support of DOE Order 430.1; Program and Project Management for the Acquisition of Capital Assets, DOE Order 413.3; the Project Execution and Engineering Management Planning Guide, GPG-FM-010; and other applicable Good Practice Guides; and the FY 2001 Integrated Planning, Accountability, and Budgeting System Policy Guidance. The plan also reflects the milestone philosophies of the Federal Facility Agreement and Consent Order, as agreed to by the State of Nevada, the DOE, and the U.S. Department of Defense; and traditional project management philosophies such as the development of life-cycle costs, schedules, and work scope; identification o f roles and responsibilities; and baseline management and controls

  15. Boundless energy. 2. rev. ed.; Grenzenlose Energie

    Energy Technology Data Exchange (ETDEWEB)

    Haege, Walter

    2007-07-01

    For thousands of years, all cultures of man have described and researched life energy, whether they called it Qi, Chi, Prana, Od or Orgon. Since Rupert Sheldrake published his theory of morphogenetic fields, the subject has been accessible to the population in general and is contributing to the decline of the current paradigm of science. Knowledge that seamed indisputable since the time of Newton and Descartes is waning, and science must restructure itself on quite a different basis. Einstein, too, is becoming history. The book spans the discoveries of Lord Kelvin to Nikola Tesla to Prof. Dr.-Ing. K. Meyl whose potential eddy theory leads to a new understanding of the world. The key to this is the neutrino, and the fundamentals of neutrino physics are quite simple: At every moment, more than 60 thousand millions of neutrinos flow through us, controlling everything that exists. In his book, author Walter Haege shows how we can all utilise this indispensable neutrino power. It is a book for everyday practice, an indispensable reference manual for all those who intend a radical change in their view of the world and their way of living. (orig.) [German] Seit Jahrtausenden wird in allen Kulturen jene Energie beschrieben und erforscht, die uns steuert und unsere Zeit am Leben erhaelt, jene Energie mit den vielen Namen, von denen uns die bekanntesten, Qi, Chi, Prana, Od oder Orgon vielleicht gelaeufig sind. Seit Rupert Sheldrake und seiner wieder aufgegriffenen Theorie vom morphogenetischen Feld ist dieses Thema grossen Bevoelkerungskreisen neu zugaenglich geworden und traegt dazu bei, dass das jetzige Paradigma der Wissenschaften seinem Ende zugeht. Unser gesichertes Wissen, seit Newton und Descartes scheinbar unumstoesslich, geht dahin und die Wissenschaft muss sich auf gaenzlich anderer Grundlage neu formieren. Auch Einstein wird Geschichte. Die Wirbelphysik Lord Kelvins fuehrt uns ueber die sensationellen Entdeckungen Nikola Teslas zu dem Physiker Prof. Dr.-Ing. K. Meyl, der uns mit seiner Theorie der Potenzialwirbel zu einem neuen Weltverstaendnis fuehrt. Der Schluessel dazu heisst Neutrinos, wobei die Grundlage der Neutrinophysik gaenzlich einfach zu verstehen ist: Mehr als 60 Milliarden Neutrinos pro Sekunde und Quadratzentimeter - aus dem Weltraum kommend - durchfliessen uns und steuern alles was ist. Walter Haege zeigt in diesem Buch praktisch auf, wie jeder von uns diese so notwendige Neutrino-Energie selbst nutzen kann. Es Ist deshalb ein Praxisbuch, dessen Inhalt unverzichtbar fuer jenen Menschen ist, der seine Sicht der Welt und damit sein gesamtes Leben radikal auf neue Fuesse stellen will. (orig.)

  16. Performance appraisal and advancement exercise 2006 (Rev)

    CERN Multimedia

    Human Resources Department

    2005-01-01

    The 2006 performance appraisal and advancement exercise will start in the usual way with annual interviews between staff and their supervisors. This year, these interviews should be held in the period from 1 December 2005 to 15 March 2006*. In this connection, a number of improvements to the procedures relating to the interviews and performance appraisals are currently under study. Administrative Circular No. 26 (Procedures Governing the Career Development of Staff Members) and the electronic MAPS form in EDH are being reviewed and will be available from December onwards. In the meantime supervisors can start the interview procedure. HR Department will shortly provide further information on this subject. The 2005 MAPS report can be retrieved for consultation at any time via EDH. * Instead of 31 March, as indicated in the electronic Bulletin No. 47 of 21 November 2005 Human Resources Department Tel. 73566

  17. Traumatological radiodiagnostics. 2. rev. and enlarged ed.

    International Nuclear Information System (INIS)

    Birzle, H.; Bergleiter, R.; Kuner, E.H.

    1985-01-01

    Traumatology accounts for a major part of diagnostic radiography. Correct application and assessment of imaging methods when examining freshly injured patients is of paramount importance and requires a good deal of experience. To this end, the book displays more than 1000 individual pictures. A brief text introduces to the peculiarities of the body region concerned. Typical injury mechanisms and available diagnostic methods are demonstrated including their advantages and disadvantages. This is followed by a comprehensive collection of pictures which also contains CT's and sonographs. With 530 figs [de

  18. Pipeline engineering. 8. rev. and enl. ed.

    International Nuclear Information System (INIS)

    Wagner, W.

    2000-01-01

    Apart from calculating the strength of pipeline components planning and design are the most important tasks on the areas of apparatus manufacturing, fluid engineering, process engineering and thermal engineering. It is therefore necessary that the flow diagrams of a plant are clearly understandable and in accordance with the technical rules even in the early stages of planning. This book concentrates on steel pipeline which are not laid underground but of the type used mostly in industrial applications. The pictures and equations provided can be used for the design of pipelines, tables and diagrams are given to facilitate estimation of elasticity, pipeline pressure losses and insulating thicknesses. An overview of the equations is given at the end of the book. Many examples facilitate learning. (orig.) [de

  19. CVD calibration light systems specifications. Rev. 0

    International Nuclear Information System (INIS)

    Mcllwain, A. K.

    1992-04-01

    Two prototype Cerenkov Viewing Device Calibration Light systems for the Mark IV CVD have been fabricated. They consist of a maintenance unit that will be used by the IAEA maintenance staff and a field unit that will be used by IAEA inspectors. More detailed information on the design of the calibration units can be obtained from the document SSP-39 and additional information on the Mark IV CVD can be obtained from the operating manual published as Canadian Safeguards Support Program document CSSP 6. The specifications refer to the prototype units which will be demonstrated to the IAEA in 1992 May. Based upon the feedback from the IAEA, the instruments will be changed in the final production models to provide devices that more closely satisfy the needs of the end users

  20. ASC FY17 Implementation Plan, Rev. 1

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton, P. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2017-06-14

    The Stockpile Stewardship Program (SSP) is an integrated technical program for maintaining the safety, surety, and reliability of the U.S. nuclear stockpile. The SSP uses nuclear test data, computational modeling and simulation, and experimental facilities to advance understanding of nuclear weapons. It includes stockpile surveillance, experimental research, development and engineering programs, and an appropriately scaled production capability to support stockpile requirements. This integrated national program requires the continued use of experimental facilities and programs, and the computational capabilities to support these programs. The Advanced Simulation and Computing Program (ASC) is a cornerstone of the SSP, providing simulation capabilities and computational resources that support annual stockpile assessment and certification, study advanced nuclear weapons design and manufacturing processes, analyze accident scenarios and weapons aging, and provide the tools to enable stockpile Life Extension Programs (LEPs) and the resolution of Significant Finding Investigations (SFIs). This requires a balance of resources, including technical staff, hardware, simulation software, and computer science solutions.

  1. Harmful effects on plants. 2. rev. ed.

    International Nuclear Information System (INIS)

    Hock, B.; Elstner, E.F.

    1988-01-01

    The book deals with accidentally or purposefully induced harmful effects on plants, i.e. eukariyotes and fungi, with emphasis being placed on chemicals as the main pollutants. After discussing the quantification of damage as a prerequisite for detecting, defining, and possibly preventing, damage to plants by toxic substances in the environment, the second part of the book deals with the harmful effects that are purposefully induced, for example in agriculture or horticulture, by employment of pesticides (herbicides or fungicides). In this context, all aspects of uptake, metabolism, and detoxication are discussed, especially referring to the molecular conditions of relevance to a selective elimination of metabolic or developmental processes in the plants, which is explained by the effects of pesticides and antibiotics. Injuries caused by autogenous substances in the plants, upon exceeding a certain amount or dose are also explained, together with effects of physical factors (as e.g. ionizing radiation), or biogenic factors (as e.g. parasites). (ECB) [de

  2. Horns Rev II, 2-D Model Tests

    DEFF Research Database (Denmark)

    Andersen, Thomas Lykke; Frigaard, Peter

    This report present the results of 2D physical model tests carried out in the shallow wave flume at Dept. of Civil Engineering, Aalborg University (AAU), on behalf of Energy E2 A/S part of DONG Energy A/S, Denmark. The objective of the tests was: to investigate the combined influence of the pile...... diameter to water depth ratio and the wave hight to water depth ratio on wave run-up of piles. The measurements should be used to design access platforms on piles....

  3. Diagnostic nuclear medicine. 2. rev. ed.

    International Nuclear Information System (INIS)

    Schiepers, C.

    2006-01-01

    The field of nuclear medicine is undergoing rapid expansion, and is evolving into diagnostic molecular imaging. During recent years, dual-modality imaging with PET/CT has gained acceptance and this is currently the fastest-growing technique for oncological imaging applications. The glucose analogue FDG has held its place in diagnostic oncology, assessment of myocardial viability and diagnosis of neuro-degenerative disorders. Peptides have become even more important as imaging agents. The accuracy of hepatobiliary scintigraphy has been enhanced by cholecystokinin. The use of ACE inhibitors in the evaluation of renovascular hypertension has become the standard in renography. New instrumentation has led to faster scanners, and computer development to better image processing software. Automatic processing is more common, and standardization of protocols can be accomplished easily. The field of gene imaging has progressed, although routine clinical applications are not yet available. The present text, supplemented with many detailed and informative illustrations, represents an adjunct to the standard knowledge of diagnostic nuclear medicine and provides both the student and the professional with an overview of developments during the past decade. (orig.)

  4. Physics, radiology and chemistry. 6. rev. ed.

    International Nuclear Information System (INIS)

    Linde, O.K.; Knigge, H.J.

    1981-01-01

    The scientific basic disciplines of physics and chemistry are the beginning of all medical teaching. They are suitable to clarify medical and biochemical problems in their causality by means of their own thinking methodics as well as by the information provided. This book attempts to point out the relationships of physics, radiology and chemistry to neighbouring disciplines, especially to practical medicine. Greater importance must naturally be given here to the examples of individual fundamental facts than to the conveying of pure theory from books. The statements and questions on self control ordered according to chapter represent a minimum learning for the students which can be extended as required. (orig./ORU) [de

  5. Radiology. Basic course 2. 4. rev. ed.

    International Nuclear Information System (INIS)

    Heilmann, A.; Steinhorst, M.

    1990-06-01

    The first part presents the subject catalogue in general radiology and a comprehensive refresher course. The main facts most frequently asked in examinations are dealt with in detail. The second part lists all important original questions from the field of general radiology, arranged by the chapters of the subject catalogue. For quick checking, correct answers are shown at the bottom of each page. The commentary presents a summary of examination problems, related subjects and problems, and hints referring to differential diagnostic aspects. The commentary part is followed by a reproduction of the original examination problems presented from March 1988 through August 1989, each collection of problems with a commentary. Students may test their state of knowledge by going through these collections of problems. There also is a collection of original images belonging to the questions. (orig./HP) With 40 figs [de

  6. Physics, radiology, and chemistry. 7. rev. ed.

    International Nuclear Information System (INIS)

    Linde, O.K.; Knigge, H.J.

    1986-01-01

    This book is an introduction to physics and chemistry especially for medical personnel. After a general introduction, measurement methods, mechanics including mechanics of solid bodies, fluids and gases, heat, optics, acoustics, electricity, radiations including their biological effects, general chemistry, inorganic and organic chemistry are treated. Every chapter contains exercises mostly in connection with medical and biological effects. Furthermore, connections with biology and medicine are considered. The chapters on physiological chemistry, computer and information theory, chemistry and ecology, and metabolism have been rewritten. (orig./HP) [de

  7. Industrial inorganic chemistry. 2. rev. ed.

    International Nuclear Information System (INIS)

    Buechner, W.; Schliebs, R.; Winter, G.; Buechel, K.H.

    1986-01-01

    Inorganic chemistry is a branch of considerable economic and technical importance. Apart from supplying the market with metals, fertilizers, building materials, pigments and glass it is one of the major suppliers of process materials to the organic chemical industry. Many modern products of other industrial sectors (video tapes, optical fibers or silicon chips) could not have been developed and manufactured without the achievements of industrial inorganic chemistry. The publication is the first of its kind to give a compact description of the inorganic chemistry sector. A clearly arranged survey facilitates access to production processes, economic aspects, ecological implications, energy consumption and raw material consumption as well as to many other data and facts. Due to its clear arrangement and the combination of technical and economic facts the book is a valuable source of information. (orig./EF) [de

  8. Fundamentals of environmental engineering. 2. rev. ed.

    International Nuclear Information System (INIS)

    Bank, M.

    1994-01-01

    'Fundamentals of Environmental Engineering' contains the technical and legal bases for the force environmental areas water supply and waste water disposal, clean air, waste avoidance and waste disposal, as well as noise protection in compact form. Particular scope was allowed for the description of the linkages between the individual environmental areas - for instance, waste combustion and clean air, waste deposition at landfills and treatment of leachate, residual products from successful water and air pollution control measures. For all those who have to familiarize themselves with the complex subject of 'environmental engineering' while in training or during continuing education this book offers a broad approach to the essential general, technical and legal bases. (orig.) [de

  9. Teaching atlas of mammography. 2. rev. ed.

    International Nuclear Information System (INIS)

    Tabar, L.; Dean, P.B.

    1985-01-01

    The purpose of this Atlas is to teach radiologists how to analyze mammograms and arrive at the correct diagnosis through proper evaluation of the findings. The illustrated cases cover practically the entire spectrum of breast abnormalities. They are based upon referred patient material as well as 80000 mammographic screening examinations. There are two basic steps in the interpretation of mammograms: perception and analysis. Since the greatest benefit of mammography lies in the detection of breast carcinoma in its earliest possible stages, every mammogram must be systematically surveyed for the subtle hints of malignancy. Perception is taught in this Atlas by describing a method for systematic viewing. The reader is then provided with a series of mammograms with obscure lesions to encourage practice with this method. With the help of a coordinate system, the lesions can be precisely located. Practice in perception continues throughout the Atlas. After detecting an abnormality on the mammogram, the diagnosis can be reached through a careful analysis of the X-ray signs. Additional projections, coned-down compression and magnetification views provide further help in this analytic workup. Rather than starting with the diagnosis and demonstrating typical findings, the approach of this Atlas is to teach the reader how to analyze the image and reach the correct diagnosis through proper evaluation of the X-ray signs. Prerequisites for the perception and evaluation of the X-ray signs are optimum technique, knowledge of anatomy and understanding of the pathological processes leading to the mammographic appearances. (orig.)

  10. 2014 SRNL LDRD Annual Report, Rev. 0

    Energy Technology Data Exchange (ETDEWEB)

    Mcwhorter, S. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2015-03-15

    Laboratory Directed Research and Development is a congressionally authorized program that provides the ‘innovation inspiration’ from which many of the Laboratory’s multi-discipline advancements are made in both science and engineering technology. The program is the backbone for insuring that scientific, technical and engineering capabilities can meet current and future needs. It is an important tool in reducing the probability of technological surprise by allowing laboratory technical staff room to innovate and keep abreast of scientific breakthroughs. Drawing from the synergism among the EM and NNSA missions, and work from other federal agencies ensures that LDRD is the key element in maintaining the vitality of SRNL’s technical programs. The LDRD program aims to position the Laboratory for new business in clean energy, national security, nuclear materials management and environmental stewardship by leveraging the unique capabilities of the Laboratory to yield foundational scientific research in core business areas, while aligning with SRS strategic initiatives and maintaining a vision for ultimate DOE applications.

  11. The ecological atlas. 3. rev. ed.

    International Nuclear Information System (INIS)

    Seager, J.

    1993-01-01

    ''The ecological atlas'' translates expert knowledge in a way that makes it accessable to the general public. In 37 double sided maps in four colours it gives information about the health of our planet and the quality of human life. Under 8 different angles: (the earth's habitat, food and drinking water, housing, energy, industry, armament, consumer needs and 'green politics'). ''The ecological atlas'' describes the effects of worldwide ecological effects: climatic disasters, the greenhouse effect, the hole in the ozone layer, destruction of the tropical rainforests, the effects of extensive farming and increasing urbanization. Pages of comprehensive commentaries complement the maps and aid understanding of their problem areas. (orig./DG) [de

  12. Radiology. 3. rev. and enl. ed.

    International Nuclear Information System (INIS)

    Reiser, Maximilian; Kuhn, Fritz-Peter; Debus, Juergen

    2011-01-01

    The text book on radiology covers the following issues: Part A: General radiology: Fundamental physics: radiation biology; radiation protection fundamentals: radiologic methods; radiotherapy; nuclear medicine. Part B: Special radiology: Thorax; heart; urogenital tract and retroperitoneum; vascular system and interventional radiology; esophagus, stomach, small and large intestines; liver, biliary system, pancreas and spleen; mammary glands; central nervous system; spinal cord and spinal canal; basis of the skull, facial bones and eye socket; neck; pediatric imaging diagnostics.

  13. Biological fundamentals. 2. completely rev. ed

    Energy Technology Data Exchange (ETDEWEB)

    Sahm, H. (ed.) (Forschungszentrum Juelich GmbH (Germany). Inst. fuer Biotechnologie)

    1993-01-01

    This fully revised and expanded Second Edition takes into account all recent developments in biotechnology. It is extraordinarily broad in scope, up-to-date, carefully structured and wellbalanced. 'Biotechnology' considers both basic concepts and widely-differing industrial applications: It is a successful synthesis of theory and practice. Any scientist aiming for success in industrial microbiology, biochemistry, molecular biology and chemical engineering must turn to 'Biotechnology'. Everybody involved in biotechnology will appreciate having this volume at their fingertips. It offers a unique collection of current information on the basic biology (ecology, taxonomy, biochemistry, physiology, and genetics) of industrially important organisms. This book contains the background material indispensable for the development of biotechnological processes. Topics included are: - Cell Structure, - Metabolism, - Growth of Microorganisms, - Metabolic Design, - Immobilized Organisms, - Methylotrophs, - Pseudomonas, - Yeasts, - Filamentous Fungi, - Bacteriophages, - Cell Cultures. (orig.)

  14. Pediatric uroradiology. 2nd rev. ed.

    International Nuclear Information System (INIS)

    Fotter, Richard

    2008-01-01

    Since the first edition of Pediatric Uroradiology, very significant advances have been made in the imaging and treatment of common and important pediatric urologic disorders such as vesicoureteric reflux, urinary tract infection, and upper urinary tract dilatation. This revised and extended version takes full account of the sometimes dramatic changes. Where appropriate, new contents have been included, e.g., on genetics, while other information that continues to be pertinent has been retained. This book describes in detail all aspects of pediatric uroradiology. While it is written primarily from the point of view of the radiologist, it includes essential information for the pediatrician, pediatric surgeon, and urologist. It is specifically designed to aid the clinician in decisions on imaging management. The newest techniques and the changing relevance of imaging and interventional procedures are presented, and the diverse problems associated with the changing anatomy, physiology, and pathophysiology from the newborn period to adulthood are explained. The whole spectrum of imaging features of agenesis, anomalies, dysplasia, parenchymal diseases, neoplastic diseases, stone disease, renal vascular hypertension, renal failure, renal transplantation, and genitourinary trauma is covered. Individual chapters are devoted to vesicoureteric reflux, urinary tract infection, upper urinary tract dilatation, voiding dysfunction, and neurogenic bladder. A new chapter on the clinical management of common nephrourologic disorders, with the subtitle ''guidelines and beyond,'' explains how imaging is embedded in the whole process of clinical management today. Short conclusions are included at the end of chapters and sections to provide the reader with the key information on the specific topic under consideration. (orig.)

  15. Pediatric uroradiology. 2nd rev. ed.

    Energy Technology Data Exchange (ETDEWEB)

    Fotter, Richard (ed.) [Univ. Hospital Medical Univ. Graz (Austria). Div. of Pediatric Radiology

    2008-07-01

    Since the first edition of Pediatric Uroradiology, very significant advances have been made in the imaging and treatment of common and important pediatric urologic disorders such as vesicoureteric reflux, urinary tract infection, and upper urinary tract dilatation. This revised and extended version takes full account of the sometimes dramatic changes. Where appropriate, new contents have been included, e.g., on genetics, while other information that continues to be pertinent has been retained. This book describes in detail all aspects of pediatric uroradiology. While it is written primarily from the point of view of the radiologist, it includes essential information for the pediatrician, pediatric surgeon, and urologist. It is specifically designed to aid the clinician in decisions on imaging management. The newest techniques and the changing relevance of imaging and interventional procedures are presented, and the diverse problems associated with the changing anatomy, physiology, and pathophysiology from the newborn period to adulthood are explained. The whole spectrum of imaging features of agenesis, anomalies, dysplasia, parenchymal diseases, neoplastic diseases, stone disease, renal vascular hypertension, renal failure, renal transplantation, and genitourinary trauma is covered. Individual chapters are devoted to vesicoureteric reflux, urinary tract infection, upper urinary tract dilatation, voiding dysfunction, and neurogenic bladder. A new chapter on the clinical management of common nephrourologic disorders, with the subtitle 'guidelines and beyond,' explains how imaging is embedded in the whole process of clinical management today. Short conclusions are included at the end of chapters and sections to provide the reader with the key information on the specific topic under consideration. (orig.)

  16. Transport of radioactive materials. 2. rev. ed.

    International Nuclear Information System (INIS)

    Vogt, H.W.; Falkhof, W.; Heibach, K.; Ungermann, N.; Hungenberg, H.

    1991-01-01

    With the last changes in the Ordinance Concerning the Transport of Hazardous Goods two regulations which are important for the carrying trade were introduced: 1. The conveyer must train the driver. He must only employ reliable drivers. 2. The driver must participate in a training course (as of July 1, 1991). These obligations, which already existed in the past in regard to the transport of nuclear fuel, have been extended to include the transport of other radioactive materials. In part I the book deals with basic training courses for parcelled goods, and part II goes into the special knowledge which is required of drivers of radioactive materials. The parts consist of the following sections: 1. General regulations, 2, Responsibility when transporting hazardous goods, 3. General danger features, 4. Information on dangers and their designation, 5. The vehicle's equipment and carrying out the transport, 6. Measures for avoiding accidents. At the end of each section the participant in the course finds a series of questions which pertain to the subject matter just treated so that he can test his own learning performance. So as to make things easier for the trainee, the corect answers are listed in the appendix. As a supplementary section on radioactive materials, part II offers additional detailed explanations by experts in the field on the features of radioactive materials and the dangers they pose. In the margin - next to the instructory text - the key words are given so that the right place in the text of the instruction manual can be readily found. These key words are compiled in the appendix to form an index. (orig./HP) [de

  17. Techniques of lipidology. 2. rev. ed.

    International Nuclear Information System (INIS)

    Kates, M.

    1986-01-01

    In this volume basic knowledge and the most reliable and useful methods and experimental procedures are collected for the study of the chemistry and metabolism of lipids. The material presented covers information necessary for the qualitative and quantitative analysis of cellular or tissue lipids, and for identification and structural determination of lipid classes or individual components. (Auth.)

  18. Environmental law. 6. rev. and enlarged ed.

    International Nuclear Information System (INIS)

    1991-01-01

    This pocketbook contains major federal regulation on environmental protection. They serve to protect and cultivate mankind's natural foundations of life, to preserve the environment. The environments law is devided as follows: Constitutional law on the environment. Common administative law on the environment, special administrative law on the environment including conservation of nature and preservation of rural amenities, protection of waters waste management, protection against nuisances, nuclear energy are radiation protection, energy conservation, protection against dangerous substances, private law relating to the environment, criminal law relating to the environment. The transitional provisons required for estaslishing the unified Germany are given in an annex. (orig.) [de

  19. Imaging pelvic floor disorders. 2. rev. ed.

    Energy Technology Data Exchange (ETDEWEB)

    Stoker, Jaap [Amsterdam Univ. (Netherlands). Dept. of Radiology; Taylor, Stuart A. [University College Hospital, London (United Kingdom). Dept. of Specialist X-Ray; DeLancey, John O.L. (eds.) [Michigan Univ., Ann Arbor, MI (United States). L4000 Women' s Hospital

    2008-07-01

    This volume builds on the success of the first edition of imaging pelvic floor disorders and is aimed at those practitioners with an interest in the imaging, diagnosis and treatment of pelvic floor dysfunction. Concise textual information from acknowledged experts is complemented by high-quality diagrams and images to provide a thorough update of this rapidly evolving field. Introductory chapters fully elucidate the anatomical basis underlying disorders of the pelvic floor. State of the art imaging techniques and their application in pelvic floor dysfunction are then discussed in detail. Additions since the first edition include consideration of the effect of aging and new chapters on perineal ultrasound, functional MRI and MRI of the levator muscles. The closing sections of the book describe the modern clinical management of pelvic floor dysfunction, including prolapse, urinary and faecal incontinence and constipation, with specific emphasis on the integration of diagnostic and treatment algorithms. (orig.)

  20. Technical fluid dynamics. 7. rev. ed.

    International Nuclear Information System (INIS)

    Becker, E.; Piltz, E.

    1993-01-01

    An introductory textbook for students of engineering containing the following subjects: Definition and properties of fluids, hydrostatics, Bernoulli's equation, theorem of momentum for steadystate flows, wing lattice and single wing, plane parallel flow of a viscous fluid, pipe flow, boundary layers, gas flows. (orig.) [de

  1. Radiology. 3. rev. and enl. ed.; Radiologie

    Energy Technology Data Exchange (ETDEWEB)

    Reiser, Maximilian [Klinikum der Universitaet Muenchen (Germany). Inst. fuer Klinische Radiologie; Kuhn, Fritz-Peter [Klinikum Kassel (Germany). Inst. fuer Diagnostische und Interventionelle Radiologie; Debus, Juergen [Radiologische Universitaetsklinik, Heidelberg (Germany). Abt. Radioonkologie und Strahlentherapie

    2011-07-01

    The text book on radiology covers the following issues: Part A: General radiology: Fundamental physics: radiation biology; radiation protection fundamentals: radiologic methods; radiotherapy; nuclear medicine. Part B: Special radiology: Thorax; heart; urogenital tract and retroperitoneum; vascular system and interventional radiology; esophagus, stomach, small and large intestines; liver, biliary system, pancreas and spleen; mammary glands; central nervous system; spinal cord and spinal canal; basis of the skull, facial bones and eye socket; neck; pediatric imaging diagnostics.

  2. Operating Manual of Helium Refrigerator (Rev. 2)

    Energy Technology Data Exchange (ETDEWEB)

    Song, K.M.; Son, S.H.; Kim, K.S.; Lee, S.K.; Kim, M.S. [Korea Electric Power Research Institute, Taejon (Korea)

    2002-07-01

    A helium refrigerator was installed as a supplier of 20K cold helium to the cryogenic distillation system of WTRF pilot plant. The operating procedures of the helium refrigerator, helium compressor and auxiliary apparatus are described for the safety and efficient operation in this manual. The function of the helium refrigerator is to remove the impurities from the compressed helium of about 250psig, to cool down the helium from ambient temperature to 20K through the heat exchanger and expansion engine and to transfer the cold helium to the cryogenic distillation system. For the smoothly operation of helium refrigerator, the preparation, the start-up, the cool-down and the shut-down of the helium refrigerator are described in this operating manual. (author). 3 refs., 14 tabs.

  3. GGVS. 7. rev. and enlarged ed.

    International Nuclear Information System (INIS)

    Ridder, K.

    1991-01-01

    The brochure contains the following texts: (1) Ordinance on road transport of hazardous materials (GGVS), including the European agreement on international road transport of hazardous materials (ADR), as of 1991: Skeleton ordinance, annexes A and B, reasons given for the first version, and for the first amendment in 1988, execution guidelines - RS 002 (guidelines for executing the ordinance on road transport of hazardous materials, with catalogue of penalties), guidelines for drawing up written instructions for the event of accidents - RS 006, guiding principles for the training of vehicle conductors; (2) ordinance regarding exceptions from the ordinance on road transport of hazardous materials; (3) ordinance regarding exceptions from the ordinance on rail transport of hazardous materials; (4) selected guidelines: Technical guidelines TR IBC K 001, TR IBL 002 - TRS 003, - TRS 004, - TRS 005, - TRS 006; (5) listing of materials and objects governed by the ordinance on hazardous materials transport; (6) catalogue of penalties relative to road transport of hazardous materials. (orig.) [de

  4. Radioactivity: Risks - safety. 2. rev. ed.

    International Nuclear Information System (INIS)

    Grupe, H.

    1991-01-01

    This revised and updated version of the report first published in 1987 by Karlsruhe Nuclear Center has been prepared by scientists of the Center who are experts in nuclear science and technology. The idea was to fulfill the so-to-speak ''creditor's'' obligation in the public debate about nuclear energy and its hazards, and to provide the general public not so familiar with the subject matter with information and insight for better understanding, hoping that the general public on their part will fulfill their ''debtor's'' obligation by trying to get properly informed. Chapters 1 and 6 of the report have been revised and brought up to date, while chapter 3 has been fully re-written, taking as a main source of reference the 1989 publication of Phase B of the German Risk Study on nuclear power plant. Chapters 2 and 5 are new in this report, dealing with less broadly known applications of radioactive substances in science, medicine and technology, and with the radiological situation in Germany after the Chernobyl reactor accident. (orig./DG) [de

  5. Reference Model 2: "Rev 0" Rotor Design

    Energy Technology Data Exchange (ETDEWEB)

    Barone, Matthew F. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Berg, Jonathan Charles [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Griffith, Daniel [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2011-12-01

    The preliminary design for a three-bladed cross-flow rotor for a reference marine hydrokinetic turbine is presented. A rotor performance design code is described, along with modifications to the code to allow prediction of blade support strut drag as well as interference between two counter-rotating rotors. The rotor is designed to operate in a reference site corresponding to a riverine environment. Basic rotor performance and rigid-body loads calculations are performed to size the rotor elements and select the operating speed range. The preliminary design is verified with a simple finite element model that provides estimates of bending stresses during operation. A concept for joining the blades and support struts is developed and analyzed with a separate finite element analysis. Rotor mass, production costs, and annual energy capture are estimated in order to allow calculations of system cost-of-energy. Evaluation Only. Created with Aspose.Pdf.Kit. Copyright 2002-2011 Aspose Pty Ltd Evaluation Only. Created with Aspose.Pdf.Kit. Copyright 2002-2011 Aspose Pty Ltd

  6. Lungs, pleura, thoracal wall. 7. rev. ed.

    International Nuclear Information System (INIS)

    Stender, H.S.

    1988-01-01

    The book describes the anatomy of the lungs, as well as X-ray, computerized tomography, nuclear magnetic resonance, and nuclear-medical imaging techniques. Following a discussion of the general symptomatology of pulmonary diseases verifiable by X-ray, the individual diseases including inhalation damage from inorganic dusts and gases are dealt with. Traumatic thoracal conditions, the image of the thorax after operations, alterations of the thoracal wall, as well as pleural diseases are also discussed. (MG) With 1776 figs., 52 tabs [de

  7. Radiation monitoring: an introduction. Rev. ed.

    International Nuclear Information System (INIS)

    Hayward, P.; Arnott, D.

    1987-01-01

    This Greenpeace pamphlet has been produced in reponse to requests from members of the public wishing to set up monitoring groups. It lists some U.K. manufacturers of radiation monitoring equipment and the contributors have summarized information available from manufacturer's own catalogues describing the equipment, what it will and will not do and costing various programmes. Three types of monitoring are discussed: monitoring the level of background gamma radiation, monitoring radioactive contamination, (early warning systems), and monitoring food and environmental samples for very low levels of radiation. (UK)

  8. Progression of visual field in patients with primary open-angle glaucoma - ProgF study 1.

    Science.gov (United States)

    Aptel, Florent; Aryal-Charles, Nishal; Giraud, Jean-Marie; El Chehab, Hussam; Delbarre, Maxime; Chiquet, Christophe; Romanet, Jean-Paul; Renard, Jean-Paul

    2015-12-01

    To evaluate the visual field rate of progression of patients with treated ocular hypertension (OHT) and primary open-angle glaucoma (POAG) in clinical practice, using the mean deviation (MD) and the visual field index (VFI). Non-interventional cohort study. From a large multicentre database representative of the French population, 441 eyes of 228 patients with treated OHT or POAG followed up at least 6 years with Humphrey 24.2 Sita-Standard visual field examination at least twice a year were identified. From initial data, eyes were classified in five groups: 121 with OHT, 188 with early glaucoma (MD greater than -6 dB), 45 with moderate glaucoma (MD -6 to -12 dB), 41 with advanced glaucoma (MD -12 to -18 dB) and 46 with severe glaucoma (MD less than -18 dB). Rate of progression during the follow-up period was calculated using the trend analysis of the Guided Progression Analysis software. The mean duration of follow-up was 8.4 ± 2.7 years and the mean number of visual field, 18.4 ± 3.5. In eyes with OHT, rate of progression was -0.09 dB/year (-0.17%VFI/year). In eyes with POAG, rate of progression was -0.32 dB/year (-0.83%VFI/year) in eyes with early glaucoma, -0.52 dB/year (-1.81%VFI/year) in moderate glaucoma, -0.54 dB/year (-2.35%VFI/year) in advanced glaucoma and -0.45 dB/year (-1.97%VFI/year) in severe glaucoma. In eyes with POAG, a significant progression (p open-angle glaucoma is a progressive disease in the majority of patients despite cautioned treatment and follow-up. The rate of progression varies greatly among subjects. © 2015 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

  9. Fundamental modes of new dispersive SH-waves in ...

    Indian Academy of Sciences (India)

    [3] M Fiebig, V V Pavlov and R V Pisarev, J. Opt. Soc. Am. B 22(1), 96 (2005). [4] T Kimura, Ann. Rev. Condens. Matter Phys. 3(1), 93 (2012). [5] Ch-S Park and Sh Priya, Advances in condensed matter physics (Hindawi Publishing. Corporation, 2012) Vol. 2012, 12 pages. [6] R C Pullar, Prog. Mater. Sci. 57(7), 1191 (2012).

  10. FINAL REPORT MELTER TESTS WITH AZ-101 HLW SIMULANT USING A DURAMELTER 100 VITRIFICATION SYSTEM VSL-01R10N0-1 REV 1 2/25/02

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; KOT WK; PEGG IL

    2011-12-29

    heat transfer in rate attainment and the much greater role of wall effects in heat transfer when the melt pool is not agitated. The DM100 melter used for the present tests has a surface area of 0.108 m{sup 2}, which is approximately 5 times larger than that of the DM10 (0.021 m{sup 2}) and approximately 11 times smaller than that of the DM1000 (1.2 m{sup 2}) (the DM1000 has since been replaced by a pilot-scale prototypical HLW melter, designated the DM1200, which has the same surface area as the DM1000). Testing on smaller melters is the most economical method for obtaining data over a wide range of operating conditions (particularly at extremes) and for guiding the more expensive tests that are performed at pilot-scale. Thus, one objective of these tests was to determine whether the DM100 melters are sufficiently large to reproduce the un-bubbled melt rates observed at the DM1000 scale, or to determine the extent of any off-set. DM100-scale tests can then be used to screen feed chemistry variations that may serve to increase the un-bubbled production rates prior to confirmation at pilot scale. Finally, extensive characterization data obtained on simulated HLW melter feeds formed from various glass forming additives indicated that there may be advantages in terms of feed rheology and stability to the replacement of some of the hydroxides by carbonates. A further objective of the present tests was therefore to identify any deleterious processing effects of such a change before adopting the carbonate feed as the baseline. Data from the WVDP melter using acidified (nitrated) feeds, and without bubbling, showed productions rates that are higher than those observed with the alkaline RPP feeds at the VSL. Therefore, the effect of feed acidification on production rate also was investigated. This work was performed under Test Specification, 'TSP-W375-00-00019, Rev 0, 'HLW-DM10 and DM100 Melter Tests' dated November 13, 2000 and the corresponding Test Plan. It

  11. Geographic Response Plan (GRP) Sensitive Site Points (Editable), Guam, 2016, US EPA Region 9

    Data.gov (United States)

    U.S. Environmental Protection Agency — This is an editable point feature data set with points over Apra Harbor in Guam. These points represent sensitive sites such as access points for public use and...

  12. Lower Colorado River GRP Drinking Water Wells, Arizona, 2012, Arizona Department of Environmental Quality

    Data.gov (United States)

    U.S. Environmental Protection Agency — The term public water system refers to any water system that has 15 or more service connections (hook-ups) or serves 25 or more people. Water systems that serve less...

  13. Method to predict fatigue lifetimes of GRP wind turbine blades and comparison with experiments

    Energy Technology Data Exchange (ETDEWEB)

    Echtermeyer, A.T. [Det Norske Veritas Research AS, Hoevik (Norway); Kensche, C. [Deutsche Forschungsanstalt fuer Luft- und Raumfahrt e.V. (DLR), Stuttgart (Germany, F.R); Bach, P. [Netherlands Energy Research Foundation (ECN), Petten (Netherlands); Poppen, M. [Aeronautical Research Inst. of Sweden, Bromma (Sweden); Lilholt, H.; Andersen, S.I.; Broendsted, P. [Risoe National Lab., Roskilde (Denmark)

    1996-12-01

    This paper describes a method to predict fatigue lifetimes of fiber reinforced plastics in wind turbine blades. It is based on extensive testing within the EU-Joule program. The method takes the measured fatigue properties of a material into account so that credit can be given to materials with improved fatigue properties. The large number of test results should also give confidence in the fatigue calculation method for fiber reinforced plastics. The method uses the Palmgren-Miner sum to predict lifetimes and is verified by tests using well defined load sequences. Even though this approach is generally well known in fatigue analysis, many details in the interpretation and extrapolation of the measurements need to be clearly defined, since they can influence the results considerably. The following subjects will be described: Method to measure SN curves and to obtain tolerance bounds, development of a constant lifetime diagram, evaluation of the load sequence, use of Palmgren-Miner sum, requirements for load sequence testing. The fatigue lifetime calculation method has been compared against measured data for simple loading sequences and the more complex WISPERX loading sequence for blade roots. The comparison is based on predicted mean lifetimes, using the same materials to obtain the basic SN curves and to measure laminates under complicated loading sequences. 24 refs, 7 figs, 5 tabs

  14. Lower Colorado River GRP Dams and Water Retention Structures, Arizona, 2012, Arizona Department of Environmental Quality

    Data.gov (United States)

    U.S. Environmental Protection Agency — Location of dams and water retention structures as compiled from multiple sources by the Arizona Department of Environmental Quality (ADEQ). The data are "sensitive"...

  15. Effects of debonds and face sheet damage in GRP sandwich panels in naval ships

    DEFF Research Database (Denmark)

    Hayman, B.; Berggreen, Christian; Quispitupa, Amilcar

    2009-01-01

    Sandwich construction with face sheets of fibre-reinforced plastics (FRP) and a core of polymer foam or balsa wood offer a lightweight construction that is well suited to naval and other vessels for high-speed operation or where payload considerations