WorldWideScience

Sample records for profile analysis procedure

  1. NASA trend analysis procedures

    Science.gov (United States)

    1993-01-01

    This publication is primarily intended for use by NASA personnel engaged in managing or implementing trend analysis programs. 'Trend analysis' refers to the observation of current activity in the context of the past in order to infer the expected level of future activity. NASA trend analysis was divided into 5 categories: problem, performance, supportability, programmatic, and reliability. Problem trend analysis uncovers multiple occurrences of historical hardware or software problems or failures in order to focus future corrective action. Performance trend analysis observes changing levels of real-time or historical flight vehicle performance parameters such as temperatures, pressures, and flow rates as compared to specification or 'safe' limits. Supportability trend analysis assesses the adequacy of the spaceflight logistics system; example indicators are repair-turn-around time and parts stockage levels. Programmatic trend analysis uses quantitative indicators to evaluate the 'health' of NASA programs of all types. Finally, reliability trend analysis attempts to evaluate the growth of system reliability based on a decreasing rate of occurrence of hardware problems over time. Procedures for conducting all five types of trend analysis are provided in this publication, prepared through the joint efforts of the NASA Trend Analysis Working Group.

  2. Pathway Analysis using Gene-expression Profiles of HPV-positive and HPV-negative Oropharyngeal Cancer Patients in a Hispanic Population: Methodological Procedures.

    Science.gov (United States)

    Suárez, Erick; González, Lorena; Pérez-Mitchell, Carlos; Ortiz, Ana P; Ramírez-Sola, Maricarmen; Acosta, Jaime; Bernabe-Dones, Raúl D; González-Aquino, Carlos; Montes-Rodríguez, Ingrid; Cadilla, Carmen L

    2016-03-01

    The incidence of oral cavity and pharyngeal cancer in Puerto Rican men is higher than it is in the men of any other ethnic/racial group in the United States of America (US). The information regarding the effect of the human papilloma virus (HPV) in the gene-expression profile among patients with this cancer is limited in Hispanic community. We aim to describe the methodology for future studies to identify the molecular networks for determining overrepresented signaling and metabolic canonical pathways, based on the differential gene-expression profiles of HPV+ and HPV- samples from patients with oropharyngeal squamous cell carcinoma in Puerto Rico. We analyzed the RNA expression of 5 tissue samples from subjects diagnosed with oropharyngeal squamous cell carcinoma, 2 HPV+ and 3 HPV-, using Affymetrix GeneChips. The relative difference between the average gene expressions of the HPV+ and HPV- samples was assessed, based on the fold change (log2-scale). Our analysis revealed 10 up regulated molecules (Mup1, LRP1, P14KA, ALYREF, and BHMT) and 5 down regulated ones (PSME4, KEAP1, ELK3, FAM186B, and PRELID1), at a cutoff of 1.5-fold change. Ingenuity Pathway Analysis showed the following biological functions to be affected in the HPV+ samples: cancer, hematological disease, and RNA post-transcriptional modification. QRT-PCR analysis confirmed only the differential regulation of ALYREF, KEAP1, and FAM186B genes. The relevant methodological procedures described are sufficient to detect the most significant biological functions and pathways according to the HPV status in patients with oropharyngeal cancer in Puerto Rico.

  3. Evaluation of vertical profiles to design continuous descent approach procedure

    Science.gov (United States)

    Pradeep, Priyank

    The current research focuses on predictability, variability and operational feasibility aspect of Continuous Descent Approach (CDA), which is among the key concepts of the Next Generation Air Transportation System (NextGen). The idle-thrust CDA is a fuel economical, noise and emission abatement procedure, but requires increased separation to accommodate for variability and uncertainties in vertical and speed profiles of arriving aircraft. Although a considerable amount of researches have been devoted to the estimation of potential benefits of the CDA, only few have attempted to explain the predictability, variability and operational feasibility aspect of CDA. The analytical equations derived using flight dynamics and Base of Aircraft and Data (BADA) Total Energy Model (TEM) in this research gives insight into dependency of vertical profile of CDA on various factors like wind speed and gradient, weight, aircraft type and configuration, thrust settings, atmospheric factors (deviation from ISA (DISA), pressure and density of the air) and descent speed profile. Application of the derived equations to idle-thrust CDA gives an insight into sensitivity of its vertical profile to multiple factors. This suggests fixed geometric flight path angle (FPA) CDA has higher degree of predictability and lesser variability at the cost of non-idle and low thrust engine settings. However, with optimized design this impact can be overall minimized. The CDA simulations were performed using Future ATM Concept Evaluation Tool (FACET) based on radar-track and aircraft type data (BADA) of the real air-traffic to some of the busiest airports in the USA (ATL, SFO and New York Metroplex (JFK, EWR and LGA)). The statistical analysis of the vertical profiles of CDA shows 1) mean geometric FPAs derived from various simulated vertical profiles are consistently shallower than 3° glideslope angle and 2) high level of variability in vertical profiles of idle-thrust CDA even in absence of

  4. Clinico-epidemiological profile and diagnostic procedures of pediatric tuberculosis in a tertiary care hospital of western Nepal-a case-series analysis

    Directory of Open Access Journals (Sweden)

    Shah Ravi K

    2010-08-01

    Full Text Available Abstract Background Changing epidemiology and diagnostic difficulties of paediatric tuberculosis (TB are being increasingly reported. Our aim was to describe clinico-epidemiological profile and diagnostic procedures used for paediatric TB. Methods A retrospective case-series analysis was carried out in a tertiary care teaching hospital of western Nepal. All pediatric TB (age 0-14 years patients registered in DOTS clinic during the time period from March, 2003 to July, 2008 were included. Medical case files were reviewed for information on demography, clinical findings, investigations and final diagnosis. Analysis was done on SPSS package. Results were expressed as rates and proportions. Chi square test was used to test for statistical significance. Results About 17.2% (162/941 of TB patients were children. Common symptoms were cough, fever and lymph node swelling. The types of TB were pulmonary TB (46.3%, 75/162, followed by extra-pulmonary TB (41.4%, 67/162. Twelve patients (7.4% had disseminated TB. Distribution of types of TB according to gender was similar. PTB was common in younger age than EPTB which was statistically significant. EPTB was mainly localized to lymph node (38, 50.7%, and abdomen (9, 12%. Five main investigations namely Mantoux test, BCG test, chest radiograph, erythrocyte sedimentation rate (ESR and fine needle aspiration cytology (FNAC or biopsy were carried out to diagnose TB. Conclusions Paediatric TB in both pulmonary and extrapulmonary forms is a common occurrence in our setting. Age incidence according to type of TB was significant. Diagnosis was based on a combination of epidemiological and clinical suspicion supported by results of various investigations.

  5. Steroid profiling in doping analysis

    NARCIS (Netherlands)

    Kerkhof, Daniël Henri van de

    2001-01-01

    Profiling androgens in urine samples is used in doping analysis for the detection of abused steroids of endogenous origin. These profiling techniques were originally developed for the analysis of testosterone, mostly by means of the ratio of testosterone to epitestosterone (T/E ratio). A study was

  6. Uniform procedure of 1H NMR analysis of rat urine and toxicometabonomics Part II : Comparison of NMR profiles classification of hepatotoxicity

    NARCIS (Netherlands)

    Schoonen, W.G.E.J.; Kloks, C.P.A.M.; Ploemen, J.-P.H.T.M.; Smit, M.J.; Zandberg, P.; Horbach, G.J.; Mellema, J.-R.; Zuylen, C.T. van; Tas, A.C.; Nesselrooij, J.H.J. van; Vogels, J.T.W.E.

    2007-01-01

    A procedure of nuclear magnetic resonance (NMR) urinalysis using pattern recognition is proposed for early detection of toxicity of investigational compounds in rats. The method is applied to detect toxicity upon administration of 13 toxic reference compounds and one nontoxic control compound

  7. HUMAN RELIABILITY ANALYSIS FOR COMPUTERIZED PROCEDURES

    Energy Technology Data Exchange (ETDEWEB)

    Ronald L. Boring; David I. Gertman; Katya Le Blanc

    2011-09-01

    This paper provides a characterization of human reliability analysis (HRA) issues for computerized procedures in nuclear power plant control rooms. It is beyond the scope of this paper to propose a new HRA approach or to recommend specific methods or refinements to those methods. Rather, this paper provides a review of HRA as applied to traditional paper-based procedures, followed by a discussion of what specific factors should additionally be considered in HRAs for computerized procedures. Performance shaping factors and failure modes unique to computerized procedures are highlighted. Since there is no definitive guide to HRA for paper-based procedures, this paper also serves to clarify the existing guidance on paper-based procedures before delving into the unique aspects of computerized procedures.

  8. Individual Profiling Using Text Analysis

    Science.gov (United States)

    2016-04-15

    likelihood that it belongs to the input text , although early experiments showed that this added no benefit. Parts–of– speech In early experiments all...tweets were POS tagged as part of the pre– processing step using a Twitter specific part–of– speech tagger [8]. Various studies have identified POS tags as...AFRL-AFOSR-UK-TR-2016-0011 Individual Profiling using Text Analysis 140333 Mark Stevenson UNIVERSITY OF SHEFFIELD, DEPARTMENT OF PSYCHOLOGY Final

  9. Development of an automated desktop procedure for defining macro-reaches for river longitudinal profiles

    CSIR Research Space (South Africa)

    Dollar, LH

    2006-07-01

    Full Text Available This paper presents an automated desktop procedure for delineating river longitudinal profiles into macro-reaches for use in Ecological Reserve assessments and to aid freshwater ecosystem conservation planning. The procedure was developed for use...

  10. L2 motivation profiling and the role of context: A study in exploratory statistical procedures

    Directory of Open Access Journals (Sweden)

    Lousia Vahtrick

    2014-07-01

    Full Text Available Two studies were carried out in order to create distinct motivational profiles of students, examine the way in which context had an effect on motivational styles, and attempt to collaborate these findings with the L2 Motivational Self-System. The research questions for this study were concerned with ascertaining whether or not it is possible to identify distinct learner types in terms of their motivational profiles; and to what extent different aspects of motivation contribute towards distinguishing different motivational profiles. The participants for the first study were 42 Dutch university students of English; and the participants for the second study were 26 adult learners of English at a language school in Sydney, Australia. In order to construct the motivational profiles, several exploratory statistical procedures were carried out, including principal components analysis and cluster analysis. The results found that the strength of the factors extracted in the principal components stage differed depending on the context of each study. In addition, the motivational profiles that were formed during the cluster analyses revealed distinct subgroups of learners which displayed varying levels of homogeneous characteristics.

  11. Computer imaging software for profile photograph analysis.

    Science.gov (United States)

    Tollefson, Travis T; Sykes, Jonathan M

    2007-01-01

    To describe a novel calibration technique for photographs of different sizes and to test a new method of chin evaluation in relation to established analysis measurements. A photograph analysis and medical record review of 14 patients who underwent combined rhinoplasty and chin correction at an academic center. Patients undergoing concurrent orthognathic surgery, rhytidectomy, or submental liposuction were excluded. Preoperative and postoperative digital photographs were analyzed using computer imaging software with a new method, the soft tissue porion to pogonion distance, and with established measurements, including the cervicomental angle, the mentocervical angle, and the facial convexity angle. The porion to pogonion distance consistently increased after the chin correction procedure (more in the osseous group). All photograph angle measurements changed toward the established normal range postoperatively. Surgery for facial disharmony requires artistic judgment and objective evaluation. Although 3-dimensional video analysis of the face seems promising, its clinical use is limited by cost. For surgeons who use computer imaging software, analysis of profile photographs is the most valuable tool. Even when preoperative and postoperative photographs are of different sizes, relative distance comparisons are possible with a new calibration technique using the constant facial landmarks, the porion and the pupil. The porion-pogonion distance is a simple reproducible measurement that can be used along with established soft tissue measurements as a guide for profile facial analysis.

  12. Computationally efficient analysis procedure for frames with ...

    African Journals Online (AJOL)

    Computationally-efficient analytical procedure that provides high-quality analysis results for two-dimensional skeletal structure with segmented (stepped) and linearly-tapered non-prismatic flexural members has been developed based on the stiffness method of structural analysis. A computer program coded in FORTRAN ...

  13. Extension Procedures for Confirmatory Factor Analysis

    Science.gov (United States)

    Nagy, Gabriel; Brunner, Martin; Lüdtke, Oliver; Greiff, Samuel

    2017-01-01

    We present factor extension procedures for confirmatory factor analysis that provide estimates of the relations of common and unique factors with external variables that do not undergo factor analysis. We present identification strategies that build upon restrictions of the pattern of correlations between unique factors and external variables. The…

  14. Deriving directions through procedural task analysis.

    Science.gov (United States)

    Yuen, H K; D'Amico, M

    1998-01-01

    Task analysis is one of the essential components of activity analysis. Procedural task analysis involves breaking down an activity into a sequence of steps. Directions are the sequence of steps resulting from the task analysis (i.e., the product of the task analysis). Directions become a guide for caregivers or trainers use in teaching clients a specific skill. However, occupational therapy students often have difficulty in writing directions that are clear enough for caregivers or trainers to carry out. Books on activity analysis only provide examples of directions without giving guidelines on how to perform the writing process. The purposes of this paper are to describe the process of procedural task analysis and to provide a guideline for writing steps of directions.

  15. Generic calibration procedures for nacelle-based profiling lidars

    DEFF Research Database (Denmark)

    Borraccino, Antoine; Courtney, Michael; Wagner, Rozenn

    In power performance testing, it has been demonstrated that the effects of wind speed and direction variations over the rotor disk can no longer be neglected for large wind turbines [1]. A new generation of commercial nacelle-based lidars is now available, offering wind profiling capabilities...... to calibrate profiling nacelle lidars....

  16. Building America House Performance Analysis Procedures

    Energy Technology Data Exchange (ETDEWEB)

    Hendron, R.; Farrar-Nagy, S.; Anderson, R.; Judkoff, R.

    2001-10-29

    As the Building America Program has grown to include a large and diverse cross section of the home building industry, accurate and consistent analysis techniques have become more important to help all program partners as they perform design tradeoffs and calculate energy savings for prototype houses built as part of the program. This document illustrates some of the analysis concepts proven effective and reliable for analyzing the transient energy usage of advanced energy systems as well as entire houses. The analysis procedure described here provides a starting point for calculating energy savings of a prototype house relative to two base cases: builder standard practice and regional standard practice. Also provides building simulation analysis to calculate annual energy savings based on side-by-side short-term field testing of a prototype house.

  17. Tear Cytokine Profile as a Noninvasive Biomarker of Inflammation for Ocular Surface Diseases: Standard Operating Procedures

    Science.gov (United States)

    Wei, Yi; Gadaria-Rathod, Neha; Epstein, Seth; Asbell, Penny

    2013-01-01

    Purpose. To provide standard operating procedures (SOPs) for measuring tear inflammatory cytokine concentrations and to validate the resulting profile as a minimally invasive objective metric and biomarker of ocular surface inflammation for use in multicenter clinical trials on dry eye disease (DED). Methods. Standard operating procedures were established and then validated with cytokine standards, quality controls, and masked tear samples collected from local and distant clinical sites. The concentrations of the inflammatory cytokines in tears were quantified using a high-sensitivity human cytokine multiplex kit. Results. A panel of inflammatory cytokines was initially investigated, from which four key inflammatory cytokines (IL-1β, IL-6, INF-γ, and TNF-α) were chosen. Results with cytokine standards statistically satisfied the manufacturer's quality control criteria. Results with pooled tear samples were highly reproducible and reliable with tear volumes ranging from 4 to 10 μL. Incorporation of the SOPs into clinical trials was subsequently validated. Tear samples were collected at a distant clinical site, stored, and shipped to our Biomarker Laboratory, where a masked analysis of the four tear cytokines was successfully performed. Tear samples were also collected from a feasibility study on DED. Inflammatory cytokine concentrations were decreased in tears of subjects who received anti-inflammatory treatment. Conclusions. Standard operating procedures for human tear cytokine assessment suitable for multicenter clinical trials were established. Tear cytokine profiling using these SOPs may provide objective metrics useful for diagnosing, classifying, and analyzing treatment efficacy in inflammatory conditions of the ocular surface, which may further elucidate the mechanisms involved in the pathogenesis of ocular surface disease. PMID:24204044

  18. Relationship between the COI test and other sensory profiles by statistical procedures

    Directory of Open Access Journals (Sweden)

    Calvente, J. J.

    1994-04-01

    Full Text Available Relationships between 139 sensory attributes evaluated on 32 samples of virgin olive oil have been analysed by a statistical sensory wheel that guarantees the objectiveness and prediction of its conclusions concerning the best clusters of attributes: green, bitter-pungent, ripe fruit, fruity, sweet fruit, undesirable attributes and two miscellanies. The procedure allows the sensory notes evaluated for potential consumers of this edible oil from the point of view of its habitual consumers to be understood with special reference to The European Communities Regulation n-2568/91. Five different panels: Spanish, Greek, Italian, Dutch and British, have been used to evaluate the samples. Analysis of the relationships between stimuli perceived by aroma, flavour, smell, mouthfeel and taste together with Linear Sensory Profiles based on Fuzzy Logic are provided. A 3-dimensional plot indicates the usefulness of the proposed procedure in the authentication of different varieties of virgin olive oil. An analysis of the volatile compounds responsible for most of the attributes gives weight to the conclusions. Directions which promise to improve the E.G. Regulation on the sensory quality of olive oil are also given.

  19. Fractal Analysis of Rock Joint Profiles

    Science.gov (United States)

    Audy, Ondřej; Ficker, Tomáš

    2017-10-01

    Surface reliefs of rock joints are analyzed in geotechnics when shear strength of rocky slopes is estimated. The rock joint profiles actually are self-affine fractal curves and computations of their fractal dimensions require special methods. Many papers devoted to the fractal properties of these profiles were published in the past but only a few of those papers employed a convenient computational method that would have guaranteed a sound value of that dimension. As a consequence, anomalously low dimensions were presented. This contribution deals with two computational modifications that lead to sound fractal dimensions of the self-affine rock joint profiles. These are the modified box-counting method and the modified yard-stick method sometimes called the compass method. Both these methods are frequently applied to self-similar fractal curves but the self-affine profile curves due to their self-affine nature require modified computational procedures implemented in computer programs.

  20. Changes in the Profile of Endovascular Procedures Performed in Freestanding Dialysis Access Centers over 15 Years.

    Science.gov (United States)

    Beathard, Gerald A; Urbanes, Aris; Litchfield, Terry

    2017-05-08

    Marked changes occurred in the vascular access profile of patients receiving hemodialysis in the United States over the 15-year period of 2001-2015. This study was undertaken to evaluate how these changes have affected dialysis access maintenance and salvage procedures performed in freestanding dialysis access centers and to examine the effectiveness, efficiency, and safety of these procedures in this setting. Data were collected from freestanding, dedicated dialysis access centers operating under a common system of management. Data were available on 689,676 dialysis access procedures. Data relating to case mix, procedure outcome, procedural time, and intraprocedural and immediate postprocedural complications were analyzed. The arteriovenous procedure profile changed from one characterized by approximately equal numbers of angioplasties and thrombectomies performed on arteriovenous grafts (AVGs) to one characterized primarily by angioplasties performed on arteriovenous fistulas. The percentage of angioplasties performed throughout the study was significantly greater than thrombectomies, with a mean of 67.9% versus 32.1% (Ppatients receiving dialysis. The incidence roughly paralleled the increasing prevalence of this type of access. A decreasing percentage of AVG utilization resulted in a progressive, roughly parallel, but disproportionately higher, decrease in the percentage of AVG procedures (Pprofile treated in freestanding, dedicated dialysis access centers changed significantly over 15 years, reflecting the changes that have occurred in the vascular access profile of the dialysis population. Copyright © 2017 by the American Society of Nephrology.

  1. Procedure for the development of a corporate profile in the CIGET of Sancti Spiritus.

    Directory of Open Access Journals (Sweden)

    Carelys Elina Sacerio Castro

    2012-04-01

    Taking into account the necessity mentioned by the CIGET and the demand of this service in the province is proposed a procedure by means of which can be characterized the entity using a corporate profile, need by the high direction as an instrument for decision making. This procedure was made having as a guide The Quality Management System that exists in the CIGET. This service will constitute one more of the services that give the CIGET.

  2. Entertainment Marketing. Occupational Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    This Occupational Competency Analysis Profile (OCAP) for entertainment marketing is an employer-verified competency list that evolved from a modified DACUM (Developing a Curriculum) job analysis process involving business, industry, labor, and community agency representatives throughout Ohio. The competency list consists of six units: (1) human…

  3. General Marketing. Occupational Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    This General Marketing Occupational Competency Analysis Profile (OCAP) is one of a series of competency lists, verified by expert workers, that have evolved from a modified DACUM (Developing a Curriculum) job analysis process involving business, industry, labor, and community agency representatives from throughout Ohio. This OCAP identifies the…

  4. Analysis Procedures for Two Station Television Meteors

    Science.gov (United States)

    Hawkes, R. L.; Mason, K. I.; Fleming, D. E. B.; Stultz, C. T.

    1993-01-01

    This paper describes techniques for trajectory, light curve and orbital analysis of image intensified television meteors recorded at two stations. It will be argued that simple partial screen reference star fitting is preferable to higher order whole screen fits, and that only modest improvements in accuracy result from additional reference stars. The coordinate transformations and triangulation procedures are expressed exclusively in vector-matrix format. A simulation program has been developed for estimation of probable errors in radiants, heights and speeds. Heights accurate to about 0.2 km can be obtained even with moderate (26 km) baselines. Because of the difficulty in defining a common fiducial point for the meteor head in different frames, velocities are usually in error by at least several percent. One technique for obtaining photometric measures from the digitized sequences will be described - accuracies of the order of +-0.20M can be obtained in a relative sense, with absolute accuracies no worse than double that value. The techniques are illustrated with actual two station data.

  5. SEMINAL FLUID ANALYSIS AND BIOPHYSICAL PROFILE: FINDINGS

    African Journals Online (AJOL)

    SEMINAL FLUID ANALYSIS AND BIOPHYSICAL PROFILE: FINDINGS. AND RELEVANCE IN INFERTILE MALES IN ILORIN, NIGERIA. lOghagbon, E. K., 2Jimoh, A. A. 6., 1Adebisi, S. A.. Department of 1Chemical Pathology / Immunology and 2Obstetrics] Gynaecology. Faculty of Health Sciences, University of Ilorin, Ilorin, ...

  6. Machine Trades. Ohio's Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    This Ohio Competency Analysis Profile (OCAP), derived from a modified Developing a Curriculum (DACUM) process, is a current comprehensive and verified employer competency program list for machine trades. Each unit (with or without subunits) contains competencies and competency builders that identify the occupational, academic, and employability…

  7. Precision Machining Technologies. Occupational Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    This Occupational Competency Analysis Profile (OCAP), which is one of a series of OCAPs developed to identify the skills that Ohio employers deem necessary to entering a given occupation/occupational area, lists the occupational, academic, and employability skills required of individuals entering the occupation of precision machinist. The…

  8. Marketing Management. Occupational Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    This Occupational Competency Analysis Profile (OCAP) contains a competency list verified by expert workers and developed through a modified DACUM (Developing a Curriculum) involving business, industry, labor, and community agency representatives from Ohio. This OCAP identifies the occupational, academic, and employability skills (competencies)…

  9. Network-based analysis of proteomic profiles

    KAUST Repository

    Wong, Limsoon

    2016-01-26

    Mass spectrometry (MS)-based proteomics is a widely used and powerful tool for profiling systems-wide protein expression changes. It can be applied for various purposes, e.g. biomarker discovery in diseases and study of drug responses. Although RNA-based high-throughput methods have been useful in providing glimpses into the underlying molecular processes, the evidences they provide are indirect. Furthermore, RNA and corresponding protein levels have been known to have poor correlation. On the other hand, MS-based proteomics tend to have consistency issues (poor reproducibility and inter-sample agreement) and coverage issues (inability to detect the entire proteome) that need to be urgently addressed. In this talk, I will discuss how these issues can be addressed by proteomic profile analysis techniques that use biological networks (especially protein complexes) as the biological context. In particular, I will describe several techniques that we have been developing for network-based analysis of proteomics profile. And I will present evidence that these techniques are useful in identifying proteomics-profile analysis results that are more consistent, more reproducible, and more biologically coherent, and that these techniques allow expansion of the detected proteome to uncover and/or discover novel proteins.

  10. Solution Tree Problem Solving Procedure for Engineering Analysis ...

    African Journals Online (AJOL)

    This paper presents a 10-step procedure, called the solution-tree problemsolving procedure, for solving engineering analysis problems. The core of the procedure is the development of a tree-like solution algorithm for the problem or class of problems, based on the divide-and-conquer and top-down design concepts.

  11. Analysis of Proximate and Protein Profile of Kefir from Fermented Goat and Cow Milk

    OpenAIRE

    Erwin Hidayat; Irna Kinayungan W; Muhammad Irhas; Fathurrahman Sidiq

    2015-01-01

    This research aims to analyze the characteristics of proximate and protein profile in kefir from fermented goat milk and cow milk with different concentration of kefir grains. The research design was true experimental with Completely Randomized Design (CRD) of 3 repetitions. The research procedures consisted of kefir production, proximate analysis and protein profile characterization. Proximate assay result was analyzed by using LSD, whereas the protein profile was analyzed by descriptive qua...

  12. A procedure for analysis of guyline tension.

    Science.gov (United States)

    Ward W. Carson; Jens E. Jorgensen; Stephen E. Reutebuch; William J. Bramwell

    1982-01-01

    Most cable logging operations use a spar held in place near the landing by a system of guylines and anchors. Safety and economic considerations require that overloads be avoided and that the spar remain stable. This paper presents a procedure and a computer program to estimate the guyline and anchor loads on a particular system configuration by a specific set of...

  13. Summative Mass Analysis of Algal Biomass - Integration of Analytical Procedures: Laboratory Analytical Procedure (LAP)

    Energy Technology Data Exchange (ETDEWEB)

    Laurens, Lieve M. L.

    2016-01-13

    This procedure guides the integration of laboratory analytical procedures to measure algal biomass constituents in an unambiguous manner and ultimately achieve mass balance closure for algal biomass samples. Many of these methods build on years of research in algal biomass analysis.

  14. Solid-phase extraction procedures in systematic toxicological analysis

    NARCIS (Netherlands)

    Franke, J.P.; de Zeeuw, R.A

    1998-01-01

    In systematic toxicological analysis (STA) the substance(s) present is (are) not known at the start of the analysis. in such an undirected search the extraction procedure cannot be directed to a given substance but must be a general procedure where a compromise must be reached in that the substances

  15. A UML Profile for State Analysis

    Science.gov (United States)

    Murray, Alex; Rasmussen, Robert

    2010-01-01

    State Analysis is a systems engineering methodology for the specification and design of control systems, developed at the Jet Propulsion Laboratory. The methodology emphasizes an analysis of the system under control in terms of States and their properties and behaviors and their effects on each other, a clear separation of the control system from the controlled system, cognizance in the control system of the controlled system's State, goal-based control built on constraining the controlled system's States, and disciplined techniques for State discovery and characterization. State Analysis (SA) introduces two key diagram types: State Effects and Goal Network diagrams. The team at JPL developed a tool for performing State Analysis. The tool includes a drawing capability, backed by a database that supports the diagram types and the organization of the elements of the SA models. But the tool does not support the usual activities of software engineering and design - a disadvantage, since systems to which State Analysis can be applied tend to be very software-intensive. This motivated the work described in this paper: the development of a preliminary Unified Modeling Language (UML) profile for State Analysis. Having this profile would enable systems engineers to specify a system using the methods and graphical language of State Analysis, which is easily linked with a larger system model in SysML (Systems Modeling Language), while also giving software engineers engaged in implementing the specified control system immediate access to and use of the SA model, in the same language, UML, used for other software design. That is, a State Analysis profile would serve as a shared modeling bridge between system and software models for the behavior aspects of the system. This paper begins with an overview of State Analysis and its underpinnings, followed by an overview of the mapping of SA constructs to the UML metamodel. It then delves into the details of these mappings and the

  16. Error analysis for mesospheric temperature profiling by absorptive occultation sensors

    Directory of Open Access Journals (Sweden)

    M. J. Rieder

    Full Text Available An error analysis for mesospheric profiles retrieved from absorptive occultation data has been performed, starting with realistic error assumptions as would apply to intensity data collected by available high-precision UV photodiode sensors. Propagation of statistical errors was investigated through the complete retrieval chain from measured intensity profiles to atmospheric density, pressure, and temperature profiles. We assumed unbiased errors as the occultation method is essentially self-calibrating and straight-line propagation of occulted signals as we focus on heights of 50–100 km, where refractive bending of the sensed radiation is negligible. Throughout the analysis the errors were characterized at each retrieval step by their mean profile, their covariance matrix and their probability density function (pdf. This furnishes, compared to a variance-only estimation, a much improved insight into the error propagation mechanism. We applied the procedure to a baseline analysis of the performance of a recently proposed solar UV occultation sensor (SMAS – Sun Monitor and Atmospheric Sounder and provide, using a reasonable exponential atmospheric model as background, results on error standard deviations and error correlation functions of density, pressure, and temperature profiles. Two different sensor photodiode assumptions are discussed, respectively, diamond diodes (DD with 0.03% and silicon diodes (SD with 0.1% (unattenuated intensity measurement noise at 10 Hz sampling rate. A factor-of-2 margin was applied to these noise values in order to roughly account for unmodeled cross section uncertainties. Within the entire height domain (50–100 km we find temperature to be retrieved to better than 0.3 K (DD / 1 K (SD accuracy, respectively, at 2 km height resolution. The results indicate that absorptive occultations acquired by a SMAS-type sensor could provide mesospheric profiles of fundamental variables such as temperature with

  17. Building America Performance Analysis Procedures: Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    None

    2004-06-01

    To measure progress toward multi-year research goals, cost and performance trade-offs are evaluated through a series of controlled field and laboratory experiments supported by energy analysis techniques using test data to calibrate simulation models.

  18. Umbilical Hernia Repair: Analysis After 934 Procedures.

    Science.gov (United States)

    Porrero, José L; Cano-Valderrama, Oscar; Marcos, Alberto; Bonachia, Oscar; Ramos, Beatriz; Alcaide, Benito; Villar, Sol; Sánchez-Cabezudo, Carlos; Quirós, Esther; Alonso, María T; Castillo, María J

    2015-09-01

    There is a lack of consensus about the surgical management of umbilical hernias. The aim of this study is to analyze the medium-term results of 934 umbilical hernia repairs. In this study, 934 patients with an umbilical hernia underwent surgery between 2004 and 2010, 599 (64.1%) of which were evaluated at least one year after the surgery. Complications, recurrence, and the reoperation rate were analyzed. Complications were observed in 5.7 per cent of the patients. With a mean follow-up time of 35.5 months, recurrence and reoperation rates were 3.8 per cent and 4.7 per cent, respectively. A higher percentage of female patients (60.9 % vs 29 %, P = 0.001) and a longer follow-up time (47.4 vs 35 months, P = 0.037) were observed in patients who developed a recurrence. No significant differences were observed between complications and the reoperation rate in patients who underwent Ventralex(®) preperitoneal mesh reinforcement and suture repair; however, a trend toward a higher recurrence rate was observed in patients with suture repair (6.5 % vs 3.2 %, P = 0.082). Suture repair had lower recurrence and reoperation rates in patients with umbilical hernias less than 1 cm. Suture repair is an appropriate procedure for small umbilical hernias; however, for larger umbilical hernias, mesh reinforcement should be considered.

  19. System analysis procedures for conducting PSA of nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Yoon Hwan; Jeong, Won Dae; Kim, Tae Un; Kim, Kil You; Han, Sang Hoon; Chang, Seung Chul; Sung, Tae Yong; Yang, Jun Eon; Kang, Dae Il; Park, Jin Hee; Hwang, Mi Jeong; Jin, Young Ho

    1997-03-01

    This document, the Probabilistic Safety Assessment(PSA) procedures guide for system analysis, is intended to provide the guidelines to analyze the target of system consistently and technically in the performance of PSA for nuclear power plants(NPPs). The guide has been prepared in accordance with the procedures and techniques for fault tree analysis(FTA) used in system analysis. Normally the main objective of system analysis is to assess the reliability of system modeled by Event Tree Analysis(ETA). A variety of analytical techniques can be used for the system analysis, however, FTA method is used in this procedures guide. FTA is the method used for representing the failure logic of plant systems deductively using AND, OR or NOT gates. The fault tree should reflect all possible failure modes that may contribute to the system unavailability. This should include contributions due to the mechanical failures of the components, Common Cause Failures (CCFs), human errors and outages for testing and maintenance. After the construction of fault tree is completed, system unavailability is calculated with the CUT module of KIRAP, and the qualitative and quantitative analysis is performed through the process as above stated. As above mentioned, the procedures for system analysis is based on PSA procedures and methods which has been applied to the safety assessments of constructing NPPs in the country. Accordingly, the method of FTA stated in this procedures guide will be applicable to PSA for the NPPs to be constructed in the future. (author). 6 tabs., 11 figs., 7 refs.

  20. Analysis and Evaluation of Current Library Procedures

    Science.gov (United States)

    Heinritz, Fred J.

    1973-01-01

    There is no shortage of appropriate techniques for the analysis of library operations. Many of those described here require no particular mathematical background, and are essentially extensions of common sense. Strengths and weaknesses of various techniques were called to attention, and wider use by librarians of certain ones was encouraged. (37…

  1. Profiling School Shooters: Automatic Text-Based Analysis

    Directory of Open Access Journals (Sweden)

    Yair eNeuman

    2015-06-01

    Full Text Available School shooters present a challenge to both forensic psychiatry and law enforcement agencies. The relatively small number of school shooters, their various charateristics, and the lack of in-depth analysis of all of the shooters prior to the shooting add complexity to our understanding of this problem. In this short paper, we introduce a new methodology for automatically profiling school shooters. The methodology involves automatic analysis of texts and the production of several measures relevant for the identification of the shooters. Comparing texts written by six school shooters to 6056 texts written by a comparison group of male subjects, we found that the shooters' texts scored significantly higher on the Narcissistic Personality dimension as well as on the Humilated and Revengeful dimensions. Using a ranking/priorization procedure, similar to the one used for the automatic identification of sexual predators, we provide support for the validity and relevance of the proposed methodology.

  2. Building America Performance Analysis Procedures: Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Hendron, R.; Anderson, R.; Judkoff, R.; Christensen, C.; Eastment, M.; Norton, P.; Reeves, P.; Hancock, E.

    2004-06-01

    To measure progress toward multi-year Building America research goals, cost and performance trade-offs are evaluated through a series of controlled field and laboratory experiments supported by energy analysis techniques that use test data to''calibrate'' energy simulation models. This report summarizes the guidelines for reporting such analytical results using the Building America Research Benchmark (Version 3.1) in studies that also include consideration of current Regional and Builder Standard Practice. Version 3.1 of the Benchmark is generally consistent with the 1999 Home Energy Rating System (HERS) Reference Home, with additions that allow evaluation of all home energy uses.

  3. Analysis of implanted silicon dopant profiles

    Energy Technology Data Exchange (ETDEWEB)

    Prosa, T.J., E-mail: ty.prosa@ametek.com [Cameca Instruments Inc., Madison, WI 53711 (United States); Olson, D.; Geiser, B.; Larson, D.J. [Cameca Instruments Inc., Madison, WI 53711 (United States); Henry, K.; Steel, E. [National Institute of Standards and Technology, Gaithersburg, MD 20899 (United States)

    2013-09-15

    Atom probe tomography implant dose measurements are reported for National Institute of Standards and Technology Standard Reference Material 2134 (arsenic implant). Efforts were taken to manufacture specimens with limited variation in size and shape to minimize variation in physical reconstruction parameters. A tip profile reconstruction was utilized where measurements of tip profile, post-analysis specimen radius and sphere-to-cone radius ratio were required as inputs into the reconstruction process. A variation of 4% is observed in the dose measurement under these conditions. Various considerations necessary to narrow the observed variation in measured dose, toward the limit imposed by counting statistics, are discussed. - Highlights: ► Multiple APT measurements were made on a NIST standard reference implant material. ► Accuracy and precision of APT was assessed on implant dose measurements. ► Dose standard deviations of ∼2% (∼2× counting statistics limit) were achieved. ► SEM exposure was found to affect the ability to successfully analyze these specimens.

  4. Accident Sequence Evaluation Program: Human reliability analysis procedure

    Energy Technology Data Exchange (ETDEWEB)

    Swain, A.D.

    1987-02-01

    This document presents a shortened version of the procedure, models, and data for human reliability analysis (HRA) which are presented in the Handbook of Human Reliability Analysis With emphasis on Nuclear Power Plant Applications (NUREG/CR-1278, August 1983). This shortened version was prepared and tried out as part of the Accident Sequence Evaluation Program (ASEP) funded by the US Nuclear Regulatory Commission and managed by Sandia National Laboratories. The intent of this new HRA procedure, called the ''ASEP HRA Procedure,'' is to enable systems analysts, with minimal support from experts in human reliability analysis, to make estimates of human error probabilities and other human performance characteristics which are sufficiently accurate for many probabilistic risk assessments. The ASEP HRA Procedure consists of a Pre-Accident Screening HRA, a Pre-Accident Nominal HRA, a Post-Accident Screening HRA, and a Post-Accident Nominal HRA. The procedure in this document includes changes made after tryout and evaluation of the procedure in four nuclear power plants by four different systems analysts and related personnel, including human reliability specialists. The changes consist of some additional explanatory material (including examples), and more detailed definitions of some of the terms. 42 refs.

  5. Profile Analysis of the Universal Nonverbal Intelligence Test Standardization Sample.

    Science.gov (United States)

    Wilhoit, Brian E.; McCallum, R. Steve

    2002-01-01

    A normative typology was developed and applied using multivariate profile analysis of subtest scores of the Universal Nonverbal Intelligence Test (UNIT) standardization sample. The results yielded a seven-profile cluster solution for the Extended Battery, and a six-profile cluster solution for the Standard Battery. Additionally, the results lend…

  6. Extended device profiles and testing procedures for the approval process of integrated medical devices using the IEEE 11073 communication standard.

    Science.gov (United States)

    Janß, Armin; Thorn, Johannes; Schmitz, Malte; Mildner, Alexander; Dell'Anna-Pudlik, Jasmin; Leucker, Martin; Radermacher, Klaus

    2018-02-23

    Nowadays, only closed and proprietary integrated operating room systems (IORS) from big manufacturers are available on the market. Hence, the interconnection of components from third-party vendors is only possible with increased time and costs. In the context of the German Federal Ministry of Education and Research (BMBF)-funded project OR.NET (2012-2016), the open integration of medical devices from different manufacturers was addressed. An integrated operating theater based on the open communication standard IEEE 11073 shall give clinical operators the opportunity to choose medical devices independently of the manufacturer. This approach would be advantageous especially for hospital operators and small- and medium-sized enterprises (SME) of medical devices. Actual standards and concepts regarding technical feasibility and the approval process do not cope with the requirements for a modular integration of medical devices in the operating room (OR), based on an open communication standard. Therefore, innovative approval strategies and corresponding certification and test procedures, which cover actual legal and normative standards, have to be developed in order to support the future risk management and the usability engineering process of open integrated medical devices in the OR. The use of standardized device and service profiles and a three-step testing procedure, including conformity, interoperability and integration tests are described in this paper and shall support the manufacturers to integrate their medical devices without disclosing the medical devices' risk analysis and related confidential expertise or proprietary information.

  7. Cost analysis of robotic versus laparoscopic general surgery procedures.

    Science.gov (United States)

    Higgins, Rana M; Frelich, Matthew J; Bosler, Matthew E; Gould, Jon C

    2017-01-01

    Robotic surgical systems have been used at a rapidly increasing rate in general surgery. Many of these procedures have been performed laparoscopically for years. In a surgical encounter, a significant portion of the total costs is associated with consumable supplies. Our hospital system has invested in a software program that can track the costs of consumable surgical supplies. We sought to determine the differences in cost of consumables with elective laparoscopic and robotic procedures for our health care organization. De-identified procedural cost and equipment utilization data were collected from the Surgical Profitability Compass Procedure Cost Manager System (The Advisory Board Company, Washington, DC) for our health care system for laparoscopic and robotic cholecystectomy, fundoplication, and inguinal hernia between the years 2013 and 2015. Outcomes were length of stay, case duration, and supply cost. Statistical analysis was performed using a t-test for continuous variables, and statistical significance was defined as p surgery procedures for our health care system when cases commonly performed laparoscopically are instead performed robotically. Our analysis is limited by the fact that we only included costs associated with consumable surgical supplies. The initial acquisition cost (over $1 million for robotic surgical system), depreciation, and service contract for the robotic and laparoscopic systems were not included in this analysis.

  8. Activated sludge morphology characterization through an image analysis procedure

    Directory of Open Access Journals (Sweden)

    Y. G. Perez

    2006-09-01

    Full Text Available This work deals with the development of a digital image analysis procedure to characterize microbial flocs obtained in three different WWTP: a bench-scale Sequencing Batch Reactor (SBR dealing with phenol and nitrogen biological removal, a municipal treatment unit (Ilha do Governador, Rio de Janeiro, Brazil and an industrial wastewater treatment plant (Ciba - Estrada do Colégio, Rio de Janeiro, Brazil. The developed procedure permits to obtain its morphological parameters like equivalent diameter, compactness, roundness and porosity properties as well as the fractal dimension. This procedure was validated and lead to identify the major relationships between the analysed morphological parameters. A minimum of 300 flocs should be included in the image analysis and a significant influence of the sample dilution step on the mean size of the flocs was verified. The porosity parameter positively correlated with the fractal dimension of microbial aggregates indicating the that highly porous flocs are very irregular.

  9. Transvenous extraction profile of Riata leads: procedural outcomes and technical complexity of mechanical removal.

    Science.gov (United States)

    Bongiorni, Maria Grazia; Di Cori, Andrea; Segreti, Luca; Zucchelli, Giulio; Viani, Stefano; Paperini, Luca; De Lucia, Raffaele; Levorato, Dianora; Boem, Adriano; Soldati, Ezio

    2015-03-01

    Riata (RT) and Sprint Fidelis (SF) leads were recalled by the United States Food and Drug Administration because of an increased rate of failure mainly due to conductor fracture or insulation abrasion. According to lead design and type of failure, extraction complexity may be different, potentially affecting procedural outcomes and indications. The purpose of this study was to assess the extraction profile of RT leads with and without cable externalization in comparison to SF leads. From January 1997 to April 2014, all consecutive RT and SF leads extracted transvenously were analyzed. Among 661 consecutive patients with 705 ventricular implantable cardioverter-defibrillator (ICD) leads extracted, 194 patients with 134 RT leads (RT group) and 61 SF leads (SF group) were identified. Removal indications often were infective (64%), and extracted leads had a prevalence of dual-coil design (89%). Baseline patients and lead characteristics were comparable between groups. Success rate was high in both groups (97.8% RT vs 100% SF) without major complications. Mechanical dilation was comparable between groups, but RT leads often required larger sheaths (11.7 ± 1.4 vs 11.3 ± 1.4), a more frequent crossover to the internal transjugular approach (14% vs 3%), and a longer procedural time (23 ± 33 minutes vs 12 ± 16 minutes). Implantation time (odds ratio 4.84, 95% confidence interval 1.05-22.2, P = .042) and RT leads (odds ratio 1.04, 95% confidence interval 1.02-1.06, P leads is feasible and effective. However, extraction of RT leads is more complex than that of SF leads. Lack of coil backfilling and cable externalization in RT group may account for these differences. Copyright © 2015 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.

  10. Effect of Purification Procedures on DIF Analysis in IRTPRO

    Science.gov (United States)

    Fikis, David R. J.; Oshima, T. C.

    2017-01-01

    Purification of the test has been a well-accepted procedure in enhancing the performance of tests for differential item functioning (DIF). As defined by Lord, purification requires reestimation of ability parameters after removing DIF items before conducting the final DIF analysis. IRTPRO 3 is a recently updated program for analyses in item…

  11. Sampling procedure for the foliar analysis of deciduous trees

    NARCIS (Netherlands)

    Luyssaert, Sebastiaan; Raitio, Hannu; Vervaeke, Pieter; Mertens, Jan; Lust, Noël

    2002-01-01

    Sampling can be the source of the greatest errors in the overall results of foliar analysis. This paper reviews the variability in heavy metal concentrations in tree crowns, which is a feature that should be known and understood when designing a suitable leaf sampling procedure. The leaf sampling

  12. Finite Element Analysis of PVC window profile &aluminium window profile with and without thermal break

    OpenAIRE

    ENG. Mohammad Buhemdi

    2016-01-01

    Examine a thermal analysis .Numerous analogies exist between thermal and structuralanalysis for PVC window profile &aluminium window profile with and without thermalbreak ,Finite Element Analysis, commonly called FEA, is a method of numerical analysis. FEA isused for solving problems in many engineering disciplines such as machine design,acoustics, electromagnetism, soil mechanics, fluid dynamics, and many others. Inmathematical terms, FEA is a numerical technique used for solving...

  13. Environmental Quality Information Analysis Center (EQIAC) operating procedures handbook

    Energy Technology Data Exchange (ETDEWEB)

    Walsh, T.E. (Florida Univ., Gainesville, FL (United States)); Das, S. (Oak Ridge National Lab., TN (United States))

    1992-08-01

    The Operating Procedures Handbook of the Environmental Quality Information Analysis Center (EQIAC) is intended to be kept current as EQIAC develops and evolves. Its purpose is to provide a comprehensive guide to the mission, infrastructure, functions, and operational procedures of EQIAC. The handbook is a training tool for new personnel and a reference manual for existing personnel. The handbook will be distributed throughout EQIAC and maintained in binders containing current dated editions of the individual sections. The handbook will be revised at least annually to reflect the current structure and operational procedures of EQIAC. The EQIAC provides information on environmental issues such as compliance, restoration, and environmental monitoring do the Air Force and DOD contractors.

  14. Quantitative standardised analysis of advanced laparoscopic surgical procedures.

    Science.gov (United States)

    Claus, G P; Sjoerdsma, W; Jansen, A; Grimbergen, C A

    1995-08-01

    To support the improvement of advanced laparoscopic surgical procedures, we designed a quantitative analysis method to monitor surgical activities. The emphasis lies on the time spent on these activities and on the instruments controlled by the hands of the surgeon. Our method uses combined video images originating from the laparoscope, an overview CCD camera placed in the operating theatre and, when available, a video colonoscope. After the operation is finished, the images are evaluated by means of a standardised analysis routine based on a spreadsheet program and a set of standard terms (thesaurus), to minimise subjectivity of the analysis. After calculations, the data are presented in tables and graphs, resulting in objective information for research on the operation. Seven advanced laparoscopic procedures, in this case colon resections, have been analysed, and it was demonstrated that the analysis method is capable of describing different laparoscopic procedures using the limited thesaurus. Possible areas of application of the method are the evaluation of time-consuming parts of the operation, of surgical tasks and measurement of the surgeon's learning curve. Other applications are the prediction and measurement of the impact of new instruments and techniques.

  15. Image analysis: basic procedures for description of plant structures.

    Science.gov (United States)

    Albrechtová, Jana; Kubínová, Zuzana; Soukup, Aleš; Janáček, Jiří

    2014-01-01

    This chapter gives examples of basic procedures of quantification of plant structures with the use of image analysis, which are commonly employed to describe differences among experimental treatments or phenotypes of plant material. Tasks are demonstrated with the use of ImageJ, a widely used public domain Java image processing program. Principles of sampling design based on systematic uniform random sampling for quantitative studies of anatomical parameters are given to obtain their unbiased estimations and simplified "rules of thumb" are presented. The basic procedures mentioned in the text are (1) sampling, (2) calibration, (3) manual length measurement, (4) leaf surface area measurement, (5) estimation of particle density demonstrated on an example of stomatal density, and (6) analysis of epidermal cell shape.

  16. Review and Application of Ship Collision and Grounding Analysis Procedures

    DEFF Research Database (Denmark)

    Pedersen, Preben Terndrup

    2010-01-01

    It is the purpose of the paper to present a review of prediction and analysis tools for collision and grounding analyses and to outline a probabilistic procedure for which these tools can be used by the maritime industry to develop performance based rules to reduce the risk associated with human,......, environmental and economic costs of collision and grounding events. The main goal of collision and grounding research should be to identify the most economic risk control options associated with prevention and mitigation of collision and grounding events.......It is the purpose of the paper to present a review of prediction and analysis tools for collision and grounding analyses and to outline a probabilistic procedure for which these tools can be used by the maritime industry to develop performance based rules to reduce the risk associated with human...

  17. General Marketing. Ohio's Competency Analysis Profile. Revised.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    Developed through a modified DACUM (Developing a Curriculum) process involving business, industry, labor, and community agency representatives in Ohio, this document is a comprehensive and verified employer competency profile for general marketing occupations. The list contains units (with and without subunits), competencies, and competency…

  18. Nurse Aide. Ohio's Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    Developed through a modified DACUM (Developing a Curriculum) process involving business, industry, labor, and community agency representatives in Ohio, this document is a comprehensive and verified employer competency profile for nurses' aides. The list contains units (with and without subunits), competencies, and competency builders that identify…

  19. Hair decontamination procedure prior to multi-class pesticide analysis.

    Science.gov (United States)

    Duca, Radu-Corneliu; Hardy, Emilie; Salquèbre, Guillaume; Appenzeller, Brice M R

    2014-06-01

    Although increasing interest is being observed in hair analysis for the biomonitoring of human exposure to pesticides, some limitations still have to be addressed for optimum use of this matrix in that specific context. One main possible issue concerns the need to differentiate chemicals biologically incorporated into hair from those externally deposited on hair surface from contaminated air or dust. The present study focuses on the development of a washing procedure for the decontamination of hair before analysis of pesticides from different chemical classes. For this purpose, three different procedures of artificial contamination (with silica, cellulose, and aqueous solution) were used to simulate pesticides deposition on hair surface. Several washing solvents (four organic: acetone, dichloromethane, methanol, acetonitrile; and four aqueous: water, phosphate buffer, shampoo, sodium dodecylsulfate) were evaluated for their capacity to remove artificially deposited pesticides from hair surface. The most effective washing solvents were sodium dodecylsulfate and methanol for aqueous and organic solvents, respectively. Moreover, after a first washing with sodium dodecylsulfate or methanol, the majority of externally deposited pesticides was removed and a steady-state was reached since significantly lower amounts were removed by additional second and third washings. Finally, the effectiveness of a decontamination procedure comprising washing with sodium dodecylsulfate and methanol was successively demonstrated. In parallel, it was determined that the final procedure did not affect the chemicals biologically incorporated, as hair strands naturally containing pesticides were used. Such a procedure appears to remove in one-shot the fraction of chemicals located on hair surface and does not require repeated washing steps. Copyright © 2014 John Wiley & Sons, Ltd.

  20. Transcriptome profiling and comparative analysis of Panax ginseng adventitious roots

    Directory of Open Access Journals (Sweden)

    Murukarthick Jayakodi

    2014-10-01

    Conclusion: This study will provide a comprehensive insight into the transcriptome of ginseng adventitious roots, and a way for successful transcriptome analysis and profiling of resource plants with less genomic information. The transcriptome profiling data generated in this study are available in our newly created adventitious root transcriptome database (http://im-crop.snu.ac.kr/transdb/index.php for public use.

  1. Goals Analysis Procedure Guidelines for Applying the Goals Analysis Process

    Science.gov (United States)

    Motley, Albert E., III

    2000-01-01

    One of the key elements to successful project management is the establishment of the "right set of requirements", requirements that reflect the true customer needs and are consistent with the strategic goals and objectives of the participating organizations. A viable set of requirements implies that each individual requirement is a necessary element in satisfying the stated goals and that the entire set of requirements, taken as a whole, is sufficient to satisfy the stated goals. Unfortunately, it is the author's experience that during project formulation phases' many of the Systems Engineering customers do not conduct a rigorous analysis of the goals and objectives that drive the system requirements. As a result, the Systems Engineer is often provided with requirements that are vague, incomplete, and internally inconsistent. To complicate matters, most systems development methodologies assume that the customer provides unambiguous, comprehensive and concise requirements. This paper describes the specific steps of a Goals Analysis process applied by Systems Engineers at the NASA Langley Research Center during the formulation of requirements for research projects. The objective of Goals Analysis is to identify and explore all of the influencing factors that ultimately drive the system's requirements.

  2. Analysis of lipid profile in lipid storage myopathy.

    Science.gov (United States)

    Aguennouz, M'hammed; Beccaria, Marco; Purcaro, Giorgia; Oteri, Marianna; Micalizzi, Giuseppe; Musumesci, Olimpia; Ciranni, Annmaria; Di Giorgio, Rosa Maria; Toscano, Antonio; Dugo, Paola; Mondello, Luigi

    2016-09-01

    Lipid dysmetabolism disease is a condition in which lipids are stored abnormally in organs and tissues throughout the body, causing muscle weakness (myopathy). Usually, the diagnosis of this disease and its characterization goes through dosage of Acyl CoA in plasma accompanied with evidence of droplets of intra-fibrils lipids in the patient muscle biopsy. However, to understand the pathophysiological mechanisms of lipid storage diseases, it is useful to identify the nature of lipids deposited in muscle fiber. In this work fatty acids and triglycerides profile of lipid accumulated in the muscle of people suffering from myopathies syndromes was characterized. In particular, the analyses were carried out on the muscle biopsy of people afflicted by lipid storage myopathy, such as multiple acyl-coenzyme A dehydrogenase deficiency, and neutral lipid storage disease with myopathy, and by the intramitochondrial lipid storage dysfunctions, such as deficiencies of carnitine palmitoyltransferase II enzyme. A single step extraction and derivatization procedure was applied to analyze fatty acids from muscle tissues by gas chromatography with a flame ionization detector and with an electronic impact mass spectrometer. Triglycerides, extracted by using n-hexane, were analyzed by high performance liquid chromatography coupled to mass spectrometer equipped with an atmospheric pressure chemical ionization interface. The most representative fatty acids in all samples were: C16:0 in the 13-24% range, C18:1n9 in the 20-52% range, and C18:2n6 in the 10-25% range. These fatty acids were part of the most representative triglycerides in all samples. The data obtained was statistically elaborated performing a principal component analysis. A satisfactory discrimination was obtained among the different diseases. Using component 1 vs component 3 a 43.3% of total variance was explained. Such results suggest the important role that lipid profile characterization can have in supporting a correct

  3. Qualitative Analysis of Commercial Social Network Profiles

    Science.gov (United States)

    Melendez, Lester; Wolfson, Ouri; Adjouadi, Malek; Rishe, Naphtali

    Social-networking sites have become an integral part of many users' daily internet routine. Commercial enterprises have been quick to recognize this and are subsequently creating profiles for many of their products and services. Commercial enterprises use social network profiles to target and interact with potential customers as well as to provide a gateway for users of the product or service to interact with each other. Many commercial enterprises use the statistics from their product or service's social network profile to tout the popularity and success of the product or service being showcased. They will use statistics such as number of friends, number of daily visits, number of interactions, and other similar measurements to quantify their claims. These statistics are often not a clear indication of the true popularity and success of the product. In this chapter the term product is used to refer to any tangible or intangible product, service, celebrity, personality, film, book, or other entity produced by a commercial enterprise.

  4. Outpatient and Inpatient Unicompartmental Knee Arthroplasty Procedures Have Similar Short-Term Complication Profiles.

    Science.gov (United States)

    Bovonratwet, Patawut; Ondeck, Nathaniel T; Tyagi, Vineet; Nelson, Stephen J; Rubin, Lee E; Grauer, Jonathan N

    2017-10-01

    Advances in surgical techniques and anesthesia have made performing unicompartmental knee arthroplasty (UKA) in the outpatient setting a possibility. The touted benefits of outpatient surgery include higher patient satisfaction and reduced costs. However, detailed information on the perioperative outcomes of outpatient compared with inpatient UKA in a large, national patient population in the United States has never been reported. The present study compares perioperative complications between outpatient and inpatient UKAs in the National Surgical Quality Improvement Program database. Patients who underwent UKA were identified in the 2005-2015 National Surgical Quality Improvement Program database. Outpatient procedures were defined as those with length of hospital stay = 0 days, whereas inpatient procedures were defined as those with length of hospital stay = 1-4 days. Patients' characteristics and comorbidities were compared between the two groups. Propensity score matched comparisons were performed for 30-day perioperative complications and readmissions between the two cohorts. This study included 568 outpatient and 5312 inpatient UKA cases. After propensity matching to control potential confounding factors, statistical analysis revealed no significant difference in any perioperative complications or any postdischarge complications between the outpatient and inpatient cohorts. Notably, the rate of 30-day readmissions between the two cohorts was not statistically different. Based on the perioperative outcome measures assessed in this study, outpatient UKA can be appropriately considered in carefully selected patients based on the lack of differences in rates of 30-day perioperative complications and readmissions between the outpatient and matched inpatient groups. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. Analysis of Elementary School students’ algebraic perceptions and procedures

    Directory of Open Access Journals (Sweden)

    Sandra Mara Marasini

    2012-12-01

    Full Text Available This study aims to verify how students in elementary school see themselves in relation to mathematics and, at the same time, analyze the procedures used to solve algebraic tasks. These students in the 8th year of elementary school, and first and third years of high school, from two State schools in Passo Fundo/RS, answered a questionnaire about their own perceptions of the mathematics lessons, the subject mathematics and algebraic content. The analysis was based mainly on authors from the athematical education and the historic-cultural psychology areas. It was verifi ed that even among students who claimed to be happy with the idea of having mathematicsclasses several presented learning diffi culties regarding algebraic contents, revealed by the procedures employed. It was concluded that it is necessary to design proposals with didactic sequences, mathematically and pedagogically based, which can effi cientlyoptimize the appropriation of meaning from the concepts approached and their application in different situations.

  6. Chunking: a procedure to improve naturalistic data analysis.

    Science.gov (United States)

    Dozza, Marco; Bärgman, Jonas; Lee, John D

    2013-09-01

    Every year, traffic accidents are responsible for more than 1,000,000 fatalities worldwide. Understanding the causes of traffic accidents and increasing safety on the road are priority issues for both legislators and the automotive industry. Recently, in Europe, the US and Japan, significant public funding has been allocated for performing large-scale naturalistic driving studies to better understand accident causation and the impact of safety systems on traffic safety. The data provided by these naturalistic driving studies has never been available before in this quantity and comprehensiveness and it promises to support a wide variety of data analyses. The volume and variety of the data also pose substantial challenges that demand new data reduction and analysis techniques. This paper presents a general procedure for the analysis of naturalistic driving data called chunking that can support many of these analyses by increasing their robustness and sensitivity. Chunking divides data into equivalent, elementary chunks of data to facilitate a robust and consistent calculation of parameters. This procedure was applied, as an example, to naturalistic driving data from the SeMiFOT study in Sweden and compared with alternative procedures from past studies in order to show its advantages and rationale in a specific example. Our results show how to apply the chunking procedure and how chunking can help avoid bias from data segments with heterogeneous durations (typically obtained from SQL queries). Finally, this paper shows how chunking can increase the robustness of parameter calculation, statistical sensitivity, and create a solid basis for further data analyses. Copyright © 2012 Elsevier Ltd. All rights reserved.

  7. An Energy Based Adaptive Pushover Analysis for Nonlinear Static Procedures

    Directory of Open Access Journals (Sweden)

    Mohsenali Shayanfar

    2016-12-01

    Full Text Available Nonlinear static procedure (NSP is a common technique to predict seismic demands on various building structures by subjecting a monotonically increasing horizontal loading (pushover to the structure. Therefore, the pushover analysis is an important part of each NSP. Accordingly, the current paper aims at investigating the efficiencyof various algorithms of lateral load patterns applied to the structure in NSPs. In recent years, fundamental advances have been made in the NSPs to enhance the response of NSPs toward nonlinear time history analysis (NTHA. Among the NSPs, the philosophy of “adaptive procedures” has been focused by many researchers. In the case of utilizing adaptive procedures, the use of incremental force vector considering the effects of higher modes of vibration and stiffness deteriorationsis possible and seems that it can lead to a good prediction of seismic response of structures. In this study, a new adaptive procedure called energy-based adaptive pushover analysis (EAPA is implemented based on the work done by modal forces in each level of the structure during the analysis and is examined for steel moment resisting frames (SMRFs. EAPA is inspired by force-based adaptive pushover (FAP and story shear-based adaptive pushover (SSAP. FAP has applied modal forces directly into load patterns; SSAP, on the other hand, has implemented the energy method in system`s capacity curve for measuring the equivalent movement. EAPA has enforced the concept of energy directly in load pattern; so that by using the modal forces-movements an energy-based adaptive algorithm is obtained. Hence, the effects of higher modes, deterioration in stiffness and strength, and characteristics of a specific site are incorporated and reflected in applied forces on the structure. Results obtained from the method proposed a desirable accordance with the extracted results from NTHA over the height of the structure.

  8. User's operating procedures. Volume 2: Scout project financial analysis program

    Science.gov (United States)

    Harris, C. G.; Haris, D. K.

    1985-01-01

    A review is presented of the user's operating procedures for the Scout Project Automatic Data system, called SPADS. SPADS is the result of the past seven years of software development on a Prime mini-computer located at the Scout Project Office, NASA Langley Research Center, Hampton, Virginia. SPADS was developed as a single entry, multiple cross-reference data management and information retrieval system for the automation of Project office tasks, including engineering, financial, managerial, and clerical support. This volume, two (2) of three (3), provides the instructions to operate the Scout Project Financial Analysis program in data retrieval and file maintenance via the user friendly menu drivers.

  9. Bile acid profiling in human biological samples: comparison of extraction procedures and application to normal and cholestatic patients.

    Science.gov (United States)

    Humbert, Lydie; Maubert, Marie Anne; Wolf, Claude; Duboc, Henri; Mahé, Myriam; Farabos, Dominique; Seksik, Philippe; Mallet, Jean Maurice; Trugnan, Germain; Masliah, Joëlle; Rainteau, Dominique

    2012-06-15

    The role of bile acids in cell metabolism, membrane biology and cell signaling is increasingly recognized, thus making necessary a robust and versatile technique to extract, separate and quantify a large concentration range of these numerous molecular species. HPLC-MS/MS analysis provides the highest sensitivity to detect and identify bile acids. However, due to their large chemical diversity, extraction methods are critical and quite difficult to optimize, as shown by a survey of the literature. This paper compares the performances of four bile acid extraction protocols applied to either liquid (serum, urine, bile) or solid (stool) samples. Acetonitrile was found to be the best solvent for deproteinizing liquid samples and NaOH the best one for stool extraction. These optimized extraction procedures allowed us to quantitate as much as 27 distinct bile acids including sulfated species in a unique 30 min HPLC run, including both hydrophilic and hydrophobic species with a high efficiency. Tandem MS provided a non ambiguous identification of each metabolite with a good sensitivity (LOQ below 20 nmol/l except for THDCA and TLCA). After validation, these methods, successfully applied to a group of 39 control patients, detected 14 different species in serum in the range of 30-800 nmol/l, 11 species in urine in the range of 20-200 nmol/l and 25 species in stool in the range of 0.4-2000 nmol/g. The clinical interest of this method has been then validated on cholestatic patients. The proposed protocols seem suitable for profiling bile acids in routine analysis. Copyright © 2012 Elsevier B.V. All rights reserved.

  10. Analysis of Near Simultaneous Jimsphere and AMPS High Resolution Wind Profiles

    Science.gov (United States)

    Adelfang, S. I.

    2003-01-01

    larger. Jimsphere radar tracking noise increases as a function of balloon displacement downrange. The Jimsphere data processing compensates for tracking signal/noise degradation by increasing the smoothing interval. The Jimsphere wind profile effective resolution is a function of downrange distance and altitude, whereas the effective resolution of the HRAMPS should be independent of those variables. The procedure used for editing Jimsphere spikes in Shuttle DOL profiles was not implemented for the Jimsphere profile measurements during the AMPS field tests. For this analysis a code was developed that essentially mimics DOL Jimsphere spike editing. Jimsphere profiles have somewhat more noise in the wavelength range less than 200m defined as the noise floor. No differences between Jimsphere and HRAMPS wind profile pairs have been found that would support denial of HRAMPS certification for application in Shuttle DOL applications. The reliability of the HRAMPS system, which is an important certification issue, is not addressed in this study.

  11. Cell-based land use screening procedure for regional siting analysis. [Utilizing spatial analysis procedures and computer graphics

    Energy Technology Data Exchange (ETDEWEB)

    Jalbert, J.S.; Dobson, J.E.

    1976-10-03

    An energy facility site-screening methodology which permits the land resource planner to identify candidate siting areas was developed. Through the use of spatial analysis procedures and computer graphics, a selection of candidate areas is obtained. Specific sites then may be selected from among candidate areas for environmental impact analysis. The computerized methodology utilizes a cell-based geographic information system for specifying the suitability of candidate areas for an energy facility. The criteria to be considered may be specified by the user and weighted in terms of importance. Three primary computer programs have been developed. These programs produce thematic maps, proximity calculations, and suitability calculations. Programs are written so as to be transferrable to regional planning or regulatory agencies to assist in rational and comprehensive power plant site identification and analysis.

  12. Shrunken head (tsantsa): a complete forensic analysis procedure.

    Science.gov (United States)

    Charlier, P; Huynh-Charlier, I; Brun, L; Hervé, C; de la Grandmaison, G Lorin

    2012-10-10

    Based on the analysis of shrunken heads referred to our forensic laboratory for anthropological expertise, and data from both anthropological and medical literature, we propose a complete forensic procedure for the analysis of such pieces. A list of 14 original morphological criteria has been developed, based on the global aspect, color, physical deformation, anatomical details, and eventual associated material (wood, vegetal fibers, sand, charcoals, etc.). Such criteria have been tested on a control sample of 20 tsantsa (i.e. shrunken heads from the Jivaro or Shuar tribes of South America). Further complementary analyses are described such as CT-scan and microscopic examination. Such expertise is more and more asked to forensic anthropologists and practitioners in a context of global repatriation of human artifacts to native communities. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  13. Analysis of River Profiles in northwestern Bhutan

    Science.gov (United States)

    de Palézieux, Larissa; Leith, Kerry; Loew, Simon

    2017-04-01

    With large alluvial plains, narrow gorges, prominent knick points, and chains of terraces or cut-off ridges, the deeply-incised valleys in Bhutan reflect an environment of diverse erosional activity. Topography ranges from 97 m to 7570 m, with characteristic postglacial landscapes typically located above ca 4200 m. The lower latitudes below ca 3000 m show high relief and terraced or linear hillslopes indicative of a fluvial origin. Although full channel analyses in the region suggest significant local tectonic contributions to longitudinal river profiles (Adams et al., 2016), we develop a method to isolate rivers in an apparently homogeneous tectonic block in the mid- to upper- elevations. Profiles of rivers in this region show a consistent pattern with a marked topographic step covering 2000 m of elevation change within 10 km. Field observations of knick points, terraces and cut-off ridges associated with the step suggest a regionally consistent signal resulting from changes in relative uplift or erosion rate. Chi plots correlate well for all channels when the base level is chosen to isolate rivers below the main alluvial plain, suggesting similar fluvial erosion histories in upstream regions. Employing third order topographic derivatives (Minár et al., 2013), we identify low angle slope sections/plateaus corresponding to terraces and/or extrapolated ridges that project onto former valley floor levels. Employing similar methods as those used to correlate fluvial knickpoints, these will be used to test for regionally consistent changes in fluvial and hillslope activity that may be tied to major tectonic or climatic changes. REFERENCES Adams, B., Whipple, K. X., Hodges, K. V. & Heimsath, A. M. 2016: In situ development of high-elevation, low-relief landscapes via duplex deformation in the Eastern Himalayan hinterland, Bhutan, in Journal of Geophysical Research: Earth Surface, 925-938. Minár, J., Jenčo, M., Evans, I. S., Minár, J., Kadlec, M., Krcho, J., Pacina

  14. Cephalometric profile of Bangladeshis: Tweed's analysis

    Directory of Open Access Journals (Sweden)

    Hasan Md Rizvi

    2017-01-01

    Full Text Available Introduction: Tweed's diagnostic triangle is simple yet provides a definite guideline in treatment planning. The aim of the present study was to establish the Tweed's norms for Bangladeshi people. Methods: The study was conducted for 89 Bangladeshi young adults (45 males and 44 females, aged 19–27 years, having balanced and harmonious facial profiles. Lateral cephalograms taken of these subjects were used for a series of morphometric analyses. Results: All three angular parameters Frankfort-mandibular plane angle (FMA, Frankfort-mandibular incisal angle (FMIA, incise mandibular plane angle (IMPAwere measured and found to be 24.52°, 54.60°, and 100.88°, respectively. The mean FMA has been found to be 24.52° (with a range of 14°–36° which is quite close to Tweed's norm and found to be statistically insignificant. However, IMPA and FMIA values of Bangladeshis found to be statistically significantly different from that of the Caucasians. The linear regression equation of IMPA on FMA was fitted, and the estimated value of IMPA was computed for a given FMA. Conclusion: The results support the idea that treatment objectives of IMPA should be considered according to the facial pattern, i.e., FMA. Ethnic variations of norms cannot be overlooked while outlining goals and planning the treatment.

  15. Food Management, Production, and Service. Occupational Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    This Food Management, Production, and Service Occupational Competency Analysis Profile (OCAP) is one of a series of competency lists, verified by expert workers, that have evolved from a modified DACUM (Developing a Curriculum) job analysis process involving business, industry, labor, and community agency representatives from throughout Ohio. This…

  16. Consumer profile analysis for different types of meat in Spain.

    Science.gov (United States)

    Escriba-Perez, Carmen; Baviera-Puig, Amparo; Buitrago-Vera, Juan; Montero-Vicente, Luis

    2017-07-01

    It is important to analyse the consumer profile of each type of meat to better adapt the marketing mix to each one. To this end, we examined the average consumption frequency of different types of meat based on two methodologies: consumer segmentation using the food-related lifestyle (FRL) framework, giving rise to 4 segments, and analysis of socio-demographic profiles. The variables used were: sex, age, educational level, social class, number of people in the household, presence of children younger than 18 in the home, geographical area and habitual residence. Beef was the only meat type significant in both analyses. Turkey meat only appeared as significant in the FRL analysis. The other meats (chicken, pork, rabbit and lamb) were only significant in the sociodemographic variables analysis. From the outcomes we may conclude that there is no single consumer profile, which rather depends on the type of meat. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Synfuel program analysis. Volume I. Procedures-capabilities

    Energy Technology Data Exchange (ETDEWEB)

    Muddiman, J. B.; Whelan, J. W.

    1980-07-01

    This is the first of the two volumes describing the analytic procedures and resulting capabilities developed by Resource Applications (RA) for examining the economic viability, public costs, and national benefits of alternative synfuel projects and integrated programs. This volume is intended for Department of Energy (DOE) and Synthetic Fuel Corporation (SFC) program management personnel and includes a general description of the costing, venture, and portfolio models with enough detail for the reader to be able to specifiy cases and interpret outputs. It also contains an explicit description (with examples) of the types of results which can be obtained when applied to: the analysis of individual projects; the analysis of input uncertainty, i.e., risk; and the analysis of portfolios of such projects, including varying technology mixes and buildup schedules. In all cases, the objective is to obtain, on the one hand, comparative measures of private investment requirements and expected returns (under differing public policies) as they affect the private decision to proceed, and, on the other, public costs and national benefits as they affect public decisions to participate (in what form, in what areas, and to what extent).

  18. Some Simple Procedures for Handling Missing Data in Multivariate Analysis

    Science.gov (United States)

    Frane, James W.

    1976-01-01

    Several procedures are outlined for replacing missing values in multivariate analyses by regression values obtained in various ways, and for adjusting coefficients (such as factor score coefficients) when data are missing. None of the procedures are complex or expensive. (Author)

  19. Modeling of precipitation and Cr depletion profiles of Inconel 600 during heat treatments and LSM procedure

    Energy Technology Data Exchange (ETDEWEB)

    Bao Gang [Department of Mechanical System Engineering, Hiroshima University, 1-4-1 Higashi-Hiroshima, Hiroshima (Japan); Shinozaki, Kenji [Department of Mechanical System Engineering, Hiroshima University, 1-4-1 Higashi-Hiroshima, Hiroshima (Japan)]. E-mail: kshino@hiroshima-u.ac.jp; Inkyo, Muneyuki [Department of Mechanical System Engineering, Hiroshima University, 1-4-1 Higashi-Hiroshima, Hiroshima (Japan); Miyoshi, Tomohisa [Department of Mechanical System Engineering, Hiroshima University, 1-4-1 Higashi-Hiroshima, Hiroshima (Japan); Yamamoto, Motomichi [Department of Mechanical System Engineering, Hiroshima University, 1-4-1 Higashi-Hiroshima, Hiroshima (Japan); Mahara, Yoichi [Babcock-Hitachi K.K., 3-36 Takara-machi, Kure, Hiroshima (Japan); Watanabe, Hiroshi [Babcock-Hitachi K.K., 3-36 Takara-machi, Kure, Hiroshima (Japan)

    2006-08-10

    A model based on the thermodynamic and kinetic was conducted to simulate the Cr depletion profiles near the grain boundary in Inconel 600 during the heat treatments and laser surface melting (LSM) process using Thermo-Calc and Dictra code. Based on the good agreement of Cr concentration distribution during heat treatments measured by experiments, the microsegregation of Cr induced by cellular microstructure formed during the LSM process was also modeled. The Cr depletion profile was evaluated using the Cr depletion area below the critical Cr concentration for intergranular cracking/intergranular stress corrosion cracking (IGC/IGSCC) susceptibility (8 mass%). Comparing with the result of Streicher test, the Cr depletion area calculated showed good coherence with the IGC/IGSCC susceptibility. The sample after SR + LTS treatment with the largest Cr depletion area showed the worst IGC/IGSCC resistance, while, the sample after LSM process with the smaller Cr depletion area showed the excellent IGC/IGSCC resistance.

  20. Extracting body image symptom dimensions among eating disorder patients: the Profile Analysis via Multidimensional Scaling (PAMS) approach.

    Science.gov (United States)

    Olatunji, Bunmi O; Kim, Se-Kang; Wall, David

    2015-09-01

    The present study employs Profile Analysis via Multidimensional Scaling (PAMS), a procedure for extracting dimensions, in order to identify core eating disorder symptoms in a clinical sample. A large sample of patients with eating disorders (N=5193) presenting for treatment completed the Eating Disorders Inventory-2 (EDI-2; Garner, 1991), and PAMS was then employed to estimate individual profile weights that reflect the degree to which an individual's observed symptom profile approximates the pattern of the dimensions. The findings revealed three symptom dimensions: Body Thinness, Body Perfectionism, and Body Awareness. Subsequent analysis using individual level data illustrate that the PAMS profiles properly operate as prototypical profiles that encapsulate all individuals' response patterns. The implications of these dimensional findings for the assessment and diagnosis of eating disorders are discussed. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. A cluster analysis of Basic Personality Inventory (BPI) adolescent profiles.

    Science.gov (United States)

    Bonynge, E R

    1994-03-01

    Basic Personality Inventory profiles of 95 male and 118 female adolescent admissions to a crisis intervention unit were subjected to a cluster analytic procedure. For both males and females, four subgroups were identified: Mental Health Maladjustment, Interpersonal Maladjustment, High-risk Rebellion, and Adjustment. Subgroups differed significantly on alternative markers of psychopathology (SCL-90-R and Diagnoses). Subgroups identified were consistent with groupings identified previously. The subgroups also corresponded with broad-band syndromes that are conventional within the literature on adolescent psychopathology. Subgroup characteristics and implications for adolescent assessment are discussed.

  2. A comprehensive analysis of surgical procedures in rural surgery practices.

    Science.gov (United States)

    Harris, Joel D; Hosford, Clint C; Sticca, Robert P

    2010-12-01

    Data regarding the practice patterns of surgeons are derived from indirect information and may not reflect practice patterns in rural surgery. The aim of this study was to analyze all procedures performed by rural surgeons in North Dakota and South Dakota in 2006. All surgeons in the Dakotas were identified by state American College of Surgeons databases. Rural urban commuting area codes were used to identify rural surgeons. Current Procedural Terminology codes from clinic, outpatient, and inpatient procedures performed during 2006 were obtained. Data were obtained from 81% of rural surgeons. A total of 46,052 Current Procedural Terminology procedure codes were analyzed. Rural surgeons averaged 1,071 procedures/year, composed of 25.6% general surgery, 39.8% endoscopy, 17.9% minor surgery, and 12.3% surgical specialty procedures. Surgeons in small and large rural communities differed in total procedures per year (1,346 vs 988). Significant differences existed in the types of procedures performed by surgeons in large and small rural communities (P surgical procedures comprising over 55% of their practices. Understanding rural surgeons' caseload will help guide the training of rural surgeons. Copyright © 2010 Elsevier Inc. All rights reserved.

  3. Variations in Academic Performance Trajectories during High School Transition: Exploring Change Profiles Via Multidimensional Scaling Growth Profile Analysis

    Science.gov (United States)

    Ding, Cody S.

    2008-01-01

    This study examines the baseline change profiles of academic performance (math and English) trajectories during the high school transition for the students who went from middle school to high school. Using multidimensional scaling growth profile analysis, we identified a no-change group plus 4 other groups with different change profile types: 1…

  4. Comparative analysis of gene expression profiles of OPN signalling ...

    Indian Academy of Sciences (India)

    The results showed that 23, 33, 59 and 74 genes were significantly changed in the above four kinds of liver diseases, respectively. H-clustering analysis showed that the expression profiles of OPN signalling-related genes were notably different in four kinds of liver diseases. Subsequently, a total of above-mentioned 147 ...

  5. Exploring Different Types of Academic Delayers: A Latent Profile Analysis

    Science.gov (United States)

    Grunschel, Carola; Patrzek, Justine; Fries, Stefan

    2013-01-01

    In this study, we explored whether there are different types of academic delayers (i.e., types of students who delay academic tasks). Latent profile analysis based on 554 university students' reasons for academic delay revealed four distinct types: inconspicuous, successful pressure-seeking, worried/anxious, and discontent with studies. The types…

  6. The Antiderivative of the Stokes V Polarization Profile. I. A Simple Procedure for Magnetic Field Characterization

    Science.gov (United States)

    Gayley, Kenneth G.

    2017-12-01

    Derived here is a more conceptually intuitive means of interpreting magnetic-field diagnostics from circularly polarized lines in a wide array of astrophysical applications. The method applies to individual “Stokes V” profile snapshots and complements standard Zeeman Doppler imaging techniques by providing the explicit form of the averaging kernel for the magnetic field that the polarization diagnostic is sensitive to. This new perspective centers on the antiderivative, or cumulative integral with respect to frequency, of the Stokes V profile. The new approach would not yield different answers for magnetic field determinations, but rather presents a more directly conceptual means of understanding the connection between what is observed and what types of fields produce it. In particular, it elucidates how lateral and line-of-sight field gradients affect the Zeeman profile. This approach is especially useful when the Zeeman shift varies in a way that correlates with the Doppler shift, as then spectral resolution serves as a proxy for spatial imaging in each polarization snapshot. Hence, the perspective is particularly useful for rapidly rotating stars, hypersonic winds, galactic rotation, and large-amplitude turbulence, when the longitudinal field varies across the source or with depth. The approach also generates an improved unsigned mean-field diagnostic that suffers less polarity cancellation than the commonly used center-of-gravity diagnostic. Reduced cancellation produces a better estimate of the field magnitude in toroidal, spotty, or dipolar fields, and a complementary comparison with the current unsigned diagnostic can help characterize the degree of field polarity reversal concealed within integrated diagnostics.

  7. AFLOWSYM: A robust procedure to perform the complete symmetry analysis of crystals

    Science.gov (United States)

    Hicks, David; Oses, Corey; Curtarolo, Stefano

    Determination of the symmetry profile of structures is a persistent challenge in materials science as evident from implementation-specific results. Herein, we present a robust procedure for evaluating the complete suite of symmetry operations, including that of the lattice point group, factor group, crystallographic point group, and space group. The protocol resolves a system-specific mapping tolerance, which accounts for variability of error in reported atomic positions. A tolerance is validated through an analysis of the operations it defines, which must all be consistent with fundamental crystallographic principles. The approach has been successfully tested against the Inorganic Crystal Structure Database (ICSD) entries cataloged in the aflow.org online repository. The AFLOWSYM package is implemented within the automatic, high-throughput computational framework AFLOW and is available for public use at aflow.org.

  8. Objective Diagnosis of Cervical Cancer by Tissue Protein Profile Analysis

    Science.gov (United States)

    Patil, Ajeetkumar; Bhat, Sujatha; Rai, Lavanya; Kartha, V. B.; Chidangil, Santhosh

    2011-07-01

    Protein profiles of homogenized normal cervical tissue samples from hysterectomy subjects and cancerous cervical tissues from biopsy samples collected from patients with different stages of cervical cancer were recorded using High Performance Liquid Chromatography coupled with Laser Induced Fluorescence (HPLC-LIF). The Protein profiles were subjected to Principle Component Analysis to derive statistically significant parameters. Diagnosis of sample types were carried out by matching three parameters—scores of factors, squared residuals, and Mahalanobis Distance. ROC and Youden's Index curves for calibration standards were used for objective estimation of the optimum threshold for decision making and performance.

  9. Defect-Related Physical-Profile-Based X-Ray and Neutron Line Profile Analysis

    Science.gov (United States)

    Ungár, Tamás; Balogh, Levente; Ribárik, Gábor

    2010-05-01

    Diffraction line broadening is caused by different defects present in crystalline materials: (1) small coherent domains, (2) dislocations, (3) other types of microstrains, (4) twin boundaries, (5) stacking faults, (6) chemical inhomogeneities, and (7) grain-to-grain second-order internal stresses. Line profile analysis provides qualitative and quantitative information about defect types and densities, respectively. Line profiles can broaden, be asymmetric, and be shifted, and these features can be anisotropic in terms of hkl indices. A few thumb rules help qualitative selection of lattice defect types. If the breadths do not increase globally with hkl, the defects are of size type, i.e., either the domain size is small or twinning or faulting, or both, is present. Whenever the breadths increase globally, the defects produce microstrains. Physically based profile functions can be determined for the different defect types and hkl anisotropy. The qualitative input about defect types based on different experimental observations allows adequate quantitative evaluation of the densities of different defect types by using physically modeled profile functions.

  10. Polar Lipid Profile of Nannochloropsis oculata Determined Using a Variety of Lipid Extraction Procedures.

    Science.gov (United States)

    Servaes, K; Maesen, M; Prandi, B; Sforza, S; Elst, K

    2015-04-22

    Lipid compositions obtained from microalgae species are affected by both the cultivation conditions and the extraction method used. In this study, the extraction of lipids from Nannochloropsis oculata using traditional and modern extraction technologies with several solvents has been compared. Because important polyunsaturated fatty acids are bound to polar lipids, these polar lipids were the main focus of this study. The dominant compounds in the glycolipid fractions were monogalactosyldiglycerides and digalactosyldiglycerides bearing fatty acid chains containing at least one site of unsaturation. Phosphatidylcholine and trimethylhomoserines were detected in the phospholipid fractions. The fatty acid profile comprised large fractions of C16:0, C16:1, C20:5, and C18:3. Extraction of specific compounds was determined by extraction efficiency as well as differences in the selectivity of the method used. The composition derived from a glycolipid fraction was observed to be affected by the method used to a greater extent than the phospholipid fraction.

  11. Analysis of Proximate and Protein Profile of Kefir from Fermented Goat and Cow Milk

    Directory of Open Access Journals (Sweden)

    Erwin Hidayat

    2015-09-01

    Full Text Available This research aims to analyze the characteristics of proximate and protein profile in kefir from fermented goat milk and cow milk with different concentration of kefir grains. The research design was true experimental with Completely Randomized Design (CRD of 3 repetitions. The research procedures consisted of kefir production, proximate analysis and protein profile characterization. Proximate assay result was analyzed by using LSD, whereas the protein profile was analyzed by descriptive qualitative method. Based on the analysis of kefir proximate levels, the kefir grain (5% showed the highest proximate level of both kefirs from goat milk and cow milk. The analysis of protein profile of cow milk kefir showed 75 kDa of protein ribbon, while the goat milk kefir showed 48 kDa, 60 kDa and 75 kDa. Therefore it can be concluded that the proximate level of goat and cow milk kefir with different concentration of kefir grains showed significant differences in the nutrition content as well as its protein profiles.Tujuan dari penelitian ini adalah menganalisis karakteristik proksimat dan profil protein pada kefir hasil fermentasi susu kambing dan susu sapi dengan konsentrasi biji kefir yang berbeda-beda. Penelitian ini adalah eksperimen murni, dengan Rancangan Acak Lengkap (RAL 3 kali ulangan. Prosedur penelitian meliputi pembuatan kefir, analisis proksimat dan profil protein. Data hasil proksimat dianalisi uji BNT, sedangkan profil protein dianalisis deskriptif kualitatif. Berdasarkan analisis kadar proksimat kefir, kefir grains 5% menunjukan kadar proksimat paling tinggi baik pada kefir susu kambing dan susu sapi. Sedangkan analisis profil protein kefir susu sapi menunjukan pita protein 75 kDa, pada kefir susu kambing yaitu 48 kDa, 60 kDa dan 75 kDa. Simpulan dari penelitian ini bahwa kadar proksimat kefir susu kambing dan susu sapi dengan konsentrasi kefir grains yang berbeda menunjukan perbedaan kandungan yang berbeda secara signifikan dengan

  12. Soil DNA extraction procedure influences protist 18S rRNA gene community profiling outcome

    DEFF Research Database (Denmark)

    Santos, Susana S.; Nunes, Ines Marques; Nielsen, Tue K.

    2017-01-01

    Advances in sequencing technologies allow deeper studies of the soil protist diversity and function. However, little attention has been given to the impact of the chosen soil DNA extraction procedure to the overall results. We examined the effect of three acknowledged DNA recovery methods, two...... manual methods (ISOm-11063, GnS-GII) and one commercial kit (MoBio), on soil protist community structures obtained from different sites with different land uses. Results from 18S rRNA gene amplicon sequencing suggest that DNA extraction method significantly affect the replicate homogeneity, the total...... number of operational taxonomic units (OTUs) recovered and the overall taxonomic structure and diversity of soil protist communities. However, DNA extraction effects did not overwhelm the natural variation among samples, as the community data still strongly grouped by geographical location...

  13. Strength analysis of welded corners of PVC window profiles

    Science.gov (United States)

    Postawa, P.; Stachowiak, T.; Gnatowski, A.

    2017-08-01

    The article presents the results of researches which main purpose was to define the influence of welding parameters on strength of welded corners of PVC window profile. PVC profiles of a branded name GENEO® produced by Rehau Company were used for this research. The profiles were made by using a co-extrusion method. The surface of the profile was made of PVC mixture with no additives. Its main task was to get a smooth surface resistant to a smudge. The use of an unfilled polyester provides an aesthetic look and improves the resistance of extrudate to water getting into inner layers. The profile's inner layers have been filled up with glass fibre in order to improve its stiffness and mechanical properties. Window frames with cut corners used for this research, were produced on technological line of EUROCOLOR Company based in Pyskowice (Poland). The main goal of this analysis was to evaluate the influence of the main welding parameter (temperature upsetting) on hardness of welds we received in whole process. A universal testing machine was used for the research.

  14. Preparation of magnetic anomaly profile and contour maps from DOE-NURE aerial survey data. Volume I: processing procedures. [National Uranium Resource Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Tinnel, E.P.; Hinze, W.J.

    1981-09-01

    Total intensity magnetic anomaly data acquired as a supplement to radiometric data in the DOE National Uranium Resource Evaluation (NURE) Program are useful in preparing regional profile and contour maps. Survey-contractor-supplied magnetic anomaly data are subjected to a multiprocess, computer-based procedure which prepares these data for presentation. This procedure is used to produce the following machine plotted maps of National Topographic Map Series quadrangle units at a 1:250,000 scale: (1) profile map of contractor-supplied magnetic anomaly data, (2) profile map of high-cut filtered data with contour levels of each profile marked and annotated on the associated flight track, (3) profile map of critical-point data with contour levels indicated, and (4) contour map of filtered and selected data. These quadrangle maps are supplemented with a range of statistical measures of the data which are useful in quality evaluation.

  15. Flash Study Analysis and The Music Learning Profiles Project

    Directory of Open Access Journals (Sweden)

    Radio Cremata

    2016-08-01

    Full Text Available This paper introduces the Music Learning Profiles Project, and its methodological approach, flash study analysis. Flash study analysis is a method that draws heavily on extant qualitative approaches to education research, to develop broad understandings of music learning in diverse contexts. The Music Learning Profiles Project (MLPP is an international collaboration to collect and curate a large number of flash studies exploring musicking and music learning in a variety of contexts that fall outside traditional school music education. In this paper the authors present context, rationale, and methods for the project, along with indicative preliminary findings. The project aims to provide an expanding online database of music experiences upon which colleagues in music education and ethnomusicology research can draw, and to which they are invited to contribute. The MLPP aims to benefit the music education community and wider society by helping to democratize research to include more diverse experiences of music learning.

  16. Lidar profilers in the context of wind energy–a verification procedure for traceable measurements

    DEFF Research Database (Denmark)

    Gottschall, Julia; Courtney, Michael; Wagner, Rozenn

    2012-01-01

    a repeatable test. Second, a linear regression is applied to the data for each height. The third step is a bin-average analysis of the lidar error, i.e. the difference between the lidar and reference measurements, forming the basis for the ensuing uncertainty estimation. The results of the verification test...

  17. Chloride ingress profiles measured by electron probe micro analysis

    DEFF Research Database (Denmark)

    Jensen, Ole mejlhede; Coats, Alison M.; Glasser, Fred P.

    1996-01-01

    Traditional techniques for measuring chloride ingress profiles do not apply well to high performance cement paste systems; the geometric resolution of the traditional measuring techniques is too low. In this paper measurements by Electron Probe Micro Analysis (EPMA) are presented. EPMA...... is demonstated to determine chloride ingress in cement paste on a micrometer scale. Potential chloride ingress routes such as cracks or the paste-aggregate interface may also be characterized by EPMA. Copyright (C) 1996 Elsevier Science Ltd...

  18. Blade Profile Optimization of Kaplan Turbine Using CFD Analysis

    Directory of Open Access Journals (Sweden)

    Aijaz Bashir Janjua

    2013-10-01

    Full Text Available Utilization of hydro-power as renewable energy source is of prime importance in the world now. Hydropower energy is available in abundant in form of falls, canals rivers, dams etc. It means, there are various types of sites with different parameters like flow rate, heads, etc. Depending upon the sites, water turbines are designed and manufactured to avail hydro-power energy. Low head turbines on runof-river are widely used for the purpose. Low head turbines are classified as reaction turbines. For runof river, depending upon the variety of site data, low head Kaplan turbines are selected, designed and manufactured. For any given site requirement, it becomes very essential to design the turbine runner blades through optimization of the CAD model of blades profile. This paper presents the optimization technique carried out on a complex geometry of blade profile through static and dynamic computational analysis. It is used through change of the blade profile geometry at five different angles in the 3D (Three Dimensional CAD model. Blade complex geometry and design have been developed by using the coordinates point system on the blade in PRO-E /CREO software. Five different blade models are developed for analysis purpose. Based on the flow rate and heads, blade profiles are analyzed using ANSYS software to check and compare the output results for optimization of the blades for improved results which show that by changing blade profile angle and its geometry, different blade sizes and geometry can be optimized using the computational techniques with changes in CAD models.

  19. Gait profile score and movement analysis profile in patients with Parkinson's disease during concurrent cognitive load

    Science.gov (United States)

    Speciali, Danielli S.; Oliveira, Elaine M.; Cardoso, Jefferson R.; Correa, João C. F.; Baker, Richard; Lucareli, Paulo R. G.

    2014-01-01

    Background: Gait disorders are common in individuals with Parkinson's Disease (PD) and the concurrent performance of motor and cognitive tasks can have marked effects on gait. The Gait Profile Score (GPS) and the Movement Analysis Profile (MAP) were developed in order to summarize the data of kinematics and facilitate understanding of the results of gait analysis. Objective: To investigate the effectiveness of the GPS and MAP in the quantification of changes in gait during a concurrent cognitive load while walking in adults with and without PD. Method: Fourteen patients with idiopathic PD and nine healthy subjects participated in the study. All subjects performed single and dual walking tasks. The GPS/MAP was computed from three-dimensional gait analysis data. Results: Differences were found between tasks for GPS (PGait Variable Score (GVS) (pelvic rotation, knee flexion-extension and ankle dorsiflexion-plantarflexion) (Pgait impairment during the dual task and suggest that GPS/MAP may be used to evaluate the effects of concurrent cognitive load while walking in patients with PD. PMID:25054382

  20. Analysis and test of low profile aluminum aerospace tank dome

    Science.gov (United States)

    Ahmed, R.; Wilhelm, J. M.

    1993-01-01

    In order to increase the structural performance of cryogenic tanks, the aerospace industry is beginning to employ low-profile bulkheads in new generation launch vehicle designs. This report details the analysis and test of one such dome made from 2219 aluminum. Such domes have two potential failure modes under internal pressure, general tensile failure and hoop compression buckling (in regions near the equator). The test determined the buckling load and ultimate tensile load of the hardware and showed that both compared well with the analysis predictions. This effort was conducted under the auspices of NASA and the General Dynamics Cryogenic Tank Technology Program (CTTP).

  1. Dose profile analysis of small fields in intensity modulated radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Medel B, E. [IMSS, Centro Medico Nacional Manuel Avila Camacho, Calle 2 Nte. 2004, Barrio de San Francisco, 72090 Puebla, Pue. (Mexico); Tejeda M, G.; Romero S, K., E-mail: romsakaren@gmail.com [Benemerita Universidad Autonoma de Puebla, Facultad de Ciencias Fisico Matematicas, Av. San Claudio y 18 Sur, Ciudad Universitaria, 72570 Puebla, Pue.(Mexico)

    2015-10-15

    Full text: Small field dosimetry is getting a very important worldwide task nowadays. The use of fields of few centimeters is more common with the introduction of sophisticated techniques of radiation therapy, as Intensity Modulated Radiotherapy (IMRT). In our country the implementation of such techniques is just getting started and whit it the need of baseline data acquisition. The dosimetry under small field conditions represents a challenge for the physicists community. In this work, a dose profile analysis was done, using various types of dosimeters for further comparisons. This analysis includes the study of quality parameters as flatness, symmetry, penumbra, and other in-axis measurements. (Author)

  2. Radiographic Parameter Analysis on Modified Sauvé-Kapandji Procedure

    Science.gov (United States)

    Ota, Norikazu; Nakamura, Toshiyasu; Iwamoto, Takuji; Sato, Kazuki; Toyama, Yoshiaki

    2013-01-01

    Purpose The Sauvé-Kapandji (S-K) procedure is now an established treatment option for symptomatic distal radioulnar joint (DRUJ) dysfunction. However, for patients with poor bone quality (frequently as a result of advanced-stage rheumatoid arthritis [RA]), the conventional S-K procedure is difficult to perform without reducing the radioulnar diameter of the wrist, which may result in a loss of grip strength and pain over the proximal ulnar stump. The purpose of this study was to review the radiographic outcomes of patients who underwent a modified S-K procedure that involves rotating the resected ulnar segment 90 degrees and using it to bridge the gap between the sigmoid notch and the ulnar head. Methods The modified S-K procedure was performed in 29 wrists of 23 patients. Twenty-one patients had severe RA, while two had malunited radius fractures. The mean follow-up period was 43 months (range, 23 to 95). The radiographic evaluation included a measurement of the radioulnar width, the pseudarthrosis gap between the proximal and distal ulnar stump, the radioulnar distance, and the ulnar translation of the carpus. Results The radioulnar width of the wrist, pseudarthrosis gap, and radioulnar distance were well maintained throughout the period. A postoperative loss in the radioulnar width of the wrists appeared to correlate with a postoperative additional ulnar translocation of the carpus. Conclusion Narrowing of the radioulnar width of the wrist is a potential cause of progressive ulnar translocation of the carpus. The modified technique for the S-K procedure maintains the distal ulna in the proper position and provides sufficient ulnar support for the carpus. It is a useful reconstruction procedure in patients with severe RA with poor bone quality. PMID:24436785

  3. [Analysis of the pragmatic abilities profile in normal preschool children].

    Science.gov (United States)

    Hage, Simone de Rocha Vasconcellos; Resegue, Marta Maria; Viveiros, Daniele Cristina Sedano de; Pacheco, Elaine Florentino

    2007-01-01

    pragmatic abilities in children. to analyze the pragmatic abilities profile in normal preschool children and to verify if significant differences exist regarding the children's different socioeconomic levels. participants of this study were 30 children, with ages between 36 and 47 months, who attended public and private elementary schools - low and medium/high socioeconomic levels respectively. A thirty minute semi-structured conversation between each child and the evaluator was recorded in VHS. For the analysis of the pragmatic abilities profile 20 minutes of each conversation was transcribed. there is a prevalence of verbal over non-verbal and unintelligible utterances; simple over expansive utterances; coherent over incoherent utterances. There was a low occurrence of utterances used to start a conversation. Regarding the use of communicative functions, the informative function prevailed, even though all the others (instrumental, heuristic, naming, narrative, negative, interactive) were used by all of the children. Comparing the performance of children who attended public schools with that of children who attended private schools, statistically significant differences were observed in the number of simple and expansive verbal utterances, and in the use of the narrative function, indicating a better performance of children from private institutions. the analysis of the children's conversational abilities profile revealed that they respond/maintain the conversation instead of initiating it; their utterances are verbal, mostly coherent and simple. Regarding the communicative functions, the most prevalent was the informative function. Sociolinguistic aspects can interfere in the pragmatic abilities of children of different socioeconomic level.

  4. A Comparative Analysis of the Procedure Employed in Item ...

    African Journals Online (AJOL)

    Thirty validated educational and psychological scales designed to measure constructs in education and social sciences were purposively selected for the study based on accessibility and availability of validation information. The instruments used for the study were scaling procedures used in 27 published journal articles ...

  5. Analysis of emergency response procedures and air traffic accidents ...

    African Journals Online (AJOL)

    Incessant air transport accidents have been a source of concern to stakeholders and aviation experts in Nigeria, yet the response and process has not been adequately appraised. This study attempts an evaluation of the emergency response procedures in the aviation industry with particular focus on Murtala Muhammed ...

  6. Stepwise Regression as an Exploratory Data Analysis Procedure.

    Science.gov (United States)

    Thayer, Jerome D.

    This paper identifies specific problems with stepwise regression, notes criticisms of stepwise methods by statisticians, suggests appropriate ways in which stepwise procedures can be used, and gives examples of how this can be done. Although the stepwise method has been routinely criticized by statisticians, it is still frequently used in the…

  7. Analysis of assistance procedures to normal birth in primiparous

    Directory of Open Access Journals (Sweden)

    Joe Luiz Vieira Garcia Novo

    2016-04-01

    Full Text Available Introduction: Current medical technologies in care in birth increased maternal and fetal benefits persist, despite numerous unnecessary procedures. The purpose of the normal childbirth care is to have healthy women and newborns, using a minimum of safe interventions. Objective: To analyze the assistance to normal delivery in secondary care maternity. Methodology: A total of 100 primiparous mothers who had vaginal delivery were included, in which care practices used were categorized: 1 according to the WHO classification for assistance to normal childbirth: effective, harmful, used with caution and used inappropriately; 2 associating calculations with the Bologna Index parameters: presence of a birth partner, partograph, no stimulation of labor, delivery in non-supine position, and mother-newborn skin-to-skin contact. Results: Birth partners (85%, correctly filled partographs (62%, mother-newborn skin-to-skin contact (36%, use of oxytocin (87%, use of parenteral nutrition during labor (86% and at delivery (74%, episiotomy (94% and uterine fundal pressure in the expulsion stage (58%. The overall average value of the Bologna Index of the mothers analyzed was 1.95. Conclusions: Some effective procedures recommended by WHO (presence of a birth partner, some effective and mandatory practices were not complied with (partograph completely filled, potentially harmful or ineffective procedures were used (oxytocin in labor/post-partum, as well as inadequate procedures (uterine fundal pressure during the expulsion stage, use of forceps and episiotomy. The maternity’s care model did not offer excellence procedures in natural birth to their mothers in primiparity, (BI=1.95.

  8. Gait profile score and movement analysis profile in patients with Parkinson's disease during concurrent cognitive load

    Directory of Open Access Journals (Sweden)

    Danielli S. Speciali

    2014-08-01

    Full Text Available Background: Gait disorders are common in individuals with Parkinson's Disease (PD and the concurrent performance of motor and cognitive tasks can have marked effects on gait. The Gait Profile Score (GPS and the Movement Analysis Profile (MAP were developed in order to summarize the data of kinematics and facilitate understanding of the results of gait analysis. Objective: To investigate the effectiveness of the GPS and MAP in the quantification of changes in gait during a concurrent cognitive load while walking in adults with and without PD. Method: Fourteen patients with idiopathic PD and nine healthy subjects participated in the study. All subjects performed single and dual walking tasks. The GPS/MAP was computed from three-dimensional gait analysis data. Results: Differences were found between tasks for GPS (P<0.05 and Gait Variable Score (GVS (pelvic rotation, knee flexion-extension and ankle dorsiflexion-plantarflexion (P<0.05 in the PD group. An interaction between task and group was observed for GPS (P<0.01 for the right side (Cohen's ¯d=0.99, left side (Cohen's ¯d=0.91, and overall (Cohen's ¯d=0.88. No interaction was observed only for hip internal-external rotation and foot internal-external progression GVS variables in the PD group. Conclusions: The results showed gait impairment during the dual task and suggest that GPS/MAP may be used to evaluate the effects of concurrent cognitive load while walking in patients with PD.

  9. Motivational orientations and imagery use: a goal profiling analysis.

    Science.gov (United States)

    Cumming, Jennifer; Hall, Craig; Harwood, Chris; Gammage, Kimberley

    2002-02-01

    The aim of this study was to establish whether different motivational profiles that result from performing a cluster analysis reflect the use of different functions and amounts of imagery. One hundred and five competitive swimmers were recruited to participate in the study. They were asked to complete both the Task and Ego Orientation in Sport Questionnaire (TEOSQ) and the Sport Imagery Questionnaire. The results of a K-means cluster analysis on the TEOSQ scores resulted in a three-cluster solution that maximized between-group differences and minimized within-group differences. A multivariate analysis of variance revealed that the three cluster groups could be distinguished by their use of imagery. Specifically, the results indicated that individuals with a 'complementary balance' between task and ego orientations were more motivated to perform the functions of imagery that would help them to maximize their performance.

  10. Profiles of adolescent religiousness using latent profile analysis: Implications for psychopathology.

    Science.gov (United States)

    Longo, Gregory S; Bray, Bethany C; Kim-Spoon, Jungmeen

    2017-03-01

    Prior research has documented robust associations between adolescent religiousness/spirituality (R/S) and psychopathology outcomes including externalizing and internalizing symptomatology, yet no previous studies have examined these associations with adolescent R/S profiles using a person-centred approach. We examined whether there are identifiable subgroups characterized by unique multidimensional patterns of R/S experiences and how these experiences may be related to externalizing and internalizing symptomatology. The sample consisted of 220 Appalachian adolescents between 12 and 18 years old who were primarily White and primarily Christian. Latent profile analysis revealed three profiles of adolescent R/S: high religiousness (28.4%), introjectors (47.6%), and low religiousness (24.0%). These profiles were differentially related to internalizing and externalizing symptomatology such that the high religiousness group was significantly lower than the introjectors with respect to internalizing and externalizing symptomatology and lower than the low religiousness group in externalizing symptomatology. Implications and suggestions for future research using person-centred approaches to better understand differential developmental trajectories of religious development are provided. Statement of contribution What is already known Prior research has demonstrated a negative relationship between adolescent religiousness and spirituality (R/S) and psychopathology. Numerous studies document the differential relationships between aspects of R/S and psychopathology; however, few have done so from a person-centred perspective. There are several theories that outline how R/S to study R/S when paying specific attention to culture. Saroglou's Big Four dimensions of religion (believing, bonding, behaving, and belonging) posits that these four dimensions (1) are able to delimit religion from proximal constructs; (2) translate major distinct dimensions of religiousness; (3) can be

  11. PROFILE ANALYSIS OF THE EMPLOYEES OF COMMERCIAL BANKS IN ROMANIA

    Directory of Open Access Journals (Sweden)

    Tudor NISTORESCU

    2012-01-01

    Full Text Available In this paper we tried to deliver a profile analysis of the employees from the commercial banks. The banking sector is especially important because it has to finance the entire economy, and therefore it needs competent and well-motivated people. As methodology we realized a survey on a representative number of employees of commercial banks. We used questionnaires measuring motivational factors, the orientation to tasks vs. people questionnaire, the potential leadership questionnaire, the Belbin self-knowledge questionnaire. The results showed that the employee from the Romanian commercial banks has a strong work-ethic and is highly motivated.

  12. [Basics of esthetic and functional cephalometric analysis of the profile].

    Science.gov (United States)

    Gola, R; Cheynet, F; Guyot, L; Richard, O

    2004-12-01

    We report a new cephalometric method for profile analysis, which uses strictly exobasicranial landmarks: 13 anatomic points, 9 bone points and 4 skin points. The analysis is based on phylogenetic, ontogenetic, anatomic and biomechanical data. Phylogenetic analysis reveals that the occipital plate belongs more to the cranial vault than the base of the skull. Embryology shows that on the midline the facial skull base ends at the spheno-occipital suture and that the overall skull base ends at the basion. The pre-maxillary fuses rapidly to the maxillary, while the pterygoid processes, which belong to the face and not the skull base, fusion very rapidly to the skull base. Revisiting the anatomy of the facial skull base shows that it is prolonged posteriorly medially to meast the synostosic creast and latterally to the glenoid fossae. Further anatomic analysis shows that the dentate and muscular part of the superior level of the facial mass correspond to equivalent parts of the inferior mandibular level. The biomechanical analysis reveals that the anterior pillar passes through the pre-maxillary, ending on the supra-orbital border and the glabella and as such belongs to the face. The posterior pillar follows the pterygoid process ending in the sphenoid. The glabella and these two pillars are taken into account in this new analysis technique.

  13. Discovering Transcriptional Modules by Combined Analysis of Expression Profiles and Regulatory Sequences

    Science.gov (United States)

    Halperin, Yonit; Linhart, Chaim; Ulitsky, Igor; Shamir, Ron

    A key goal of gene expression analysis is the characterization of transcription factors (TFs) and micro-RNAs (miRNAs) regulating specific transcriptional programs. The most common approach to address this task is a two-step methodology: In the first step, a clustering procedure is executed to partition the genes into groups that are believed to be co-regulated, based on expression profile similarity. In the second step, a motif discovery tool is applied to search for over-represented cis-regulatory motifs within each group. In an effort to obtain better results by simultaneously utilizing all available information, several studies have suggested computational schemes for a single-step combined analysis of expression and sequence data. Despite extensive research, reverse engineering complex regulatory networks from microarray measurements remains a difficult challenge with limited success, especially in metazoans.

  14. Comparison of Three Criteria for Discriminant Analysis Procedure ...

    African Journals Online (AJOL)

    This paper presents a fisher's criterion, Welch's criterion, and Bayes criterion for performing a discriminant analysis. These criteria estimates a linear discriminant analysis on two groups (or regions) of contrived observations. The discriminant functions and classification rules for these criteria are also discussed. A linear ...

  15. Parent Prevention Communication Profiles and Adolescent Substance Use: A Latent Profile Analysis and Growth Curve Model

    Science.gov (United States)

    Choi, Hye Jeong; Miller-Day, Michelle; Shin, YoungJu; Hecht, Michael L.; Pettigrew, Jonathan; Krieger, Janice L.; Lee, JeongKyu; Graham, John W.

    2017-01-01

    This current study identifies distinct parent prevention communication profiles and examines whether youth with different parental communication profiles have varying substance use trajectories over time. Eleven schools in two rural school districts in the Midwestern United States were selected, and 784 students were surveyed at three time points from the beginning of 7th grade to the end of 8th grade. A series of latent profile analyses were performed to identify discrete profiles/subgroups of substance-specific prevention communication (SSPC). The results revealed a 4-profile model of SSPC: Active-Open, Passive-Open, Active-Silent, and Passive-Silent. A growth curve model revealed different rates of lifetime substance use depending on the youth’s SSPC profile. These findings have implications for parenting interventions and tailoring messages for parents to fit specific SSPC profiles. PMID:29056872

  16. Which surgical procedure is effective for refractory chronic subdural hematoma? Analysis of our surgical procedures and literature review.

    Science.gov (United States)

    Matsumoto, Hiroaki; Hanayama, Hiroaki; Okada, Takashi; Sakurai, Yasuo; Minami, Hiroaki; Masuda, Atsushi; Tominaga, Shogo; Miyaji, Katsuya; Yamaura, Ikuya; Yoshida, Yasuhisa

    2018-03-01

    Refractory chronic subdural hematoma (CSDH) is rare but remains a difficulty for neurosurgeons, and no consensus on treatment procedures has been established. To discuss effective surgical procedures for refractory CSDH, we analyzed our surgical procedures and outcomes for refractory CSDH. We defined patients with refractory CSDH as those who presented with two or more recurrences. Fourteen patients with refractory CSDH were analyzed. Eight patients underwent burr-hole irrigation and closed-system drainage alone, four patients received embolization of the middle meningeal artery (MMA), and two patients with organized CSDH underwent large craniotomy with outer membranectomy as the third surgery. Two of the eight patients (25%) treated with burr-hole irrigation and drainage alone showed a third recurrence. No further recurrences were identified in patients treated with embolization of the MMA or craniotomy. However, statistical analysis showed no significant difference in cure rate between patients treated with burr-hole irrigation and drainage alone and patients treated with burr-hole irrigation and drainage with embolization of the MMA (P = .42). Similarly, no significant differences in cure rate were seen between patients treated with burr-hole irrigation and drainage alone and patients treated with craniotomy (P = .62). When selecting a surgical procedure, assessing whether the CSDH is organized is crucial. Embolization of the MMA may be considered as one of the optional treatments for refractory CSDH without organized hematoma. On the other hand, for refractory cases of organized CSDH, hematoma evacuation and outer membranectomy with large craniotomy or mini-craniotomy assisted by an endoscope may be suitable, as previous reports have recommended. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Analysis of the pharyngoesophageal pressure profile in amyotrophic lateral sclerosis.

    Science.gov (United States)

    MacDougall, G; Wilson, J A; Pryde, A; Grant, R

    1995-02-01

    Treatment of dysphagia resulting from bulbar amyotrophic lateral sclerosis has included cricopharyngeal myotomy for many years but is successful in only a minority of cases. The purpose of this study was to explore the rationale for this procedure with modern manometric techniques. The results of pharyngoesophageal manometry in 13 patients with amyotrophic lateral sclerosis were compared with 13 age- and sex-matched healthy volunteers by Mann-Whitney analysis. There was no significant difference between patients and control subjects in distal esophageal or lower esophageal sphincter motility nor any pressure parameter of pharyngoesophageal motility. Separate analysis of the seven significantly dysphagic subjects showed a significantly reduced upper esophageal sphincter after-contraction amplitude during water and bread swallows in patients than in control subjects. These data suggest that the dysphagia of amyotrophic lateral sclerosis is not due to upper esophageal sphincter spasm and that treatment by cricopharyngeal myotomy may be inappropriate.

  18. Mutational Profile of Metastatic Breast Cancers: A Retrospective Analysis.

    Directory of Open Access Journals (Sweden)

    Celine Lefebvre

    2016-12-01

    Full Text Available Major advances have been achieved in the characterization of early breast cancer (eBC genomic profiles. Metastatic breast cancer (mBC is associated with poor outcomes, yet limited information is available on the genomic profile of this disease. This study aims to decipher mutational profiles of mBC using next-generation sequencing.Whole-exome sequencing was performed on 216 tumor-blood pairs from mBC patients who underwent a biopsy in the context of the SAFIR01, SAFIR02, SHIVA, or Molecular Screening for Cancer Treatment Optimization (MOSCATO prospective trials. Mutational profiles from 772 primary breast tumors from The Cancer Genome Atlas (TCGA were used as a reference for comparing primary and mBC mutational profiles. Twelve genes (TP53, PIK3CA, GATA3, ESR1, MAP3K1, CDH1, AKT1, MAP2K4, RB1, PTEN, CBFB, and CDKN2A were identified as significantly mutated in mBC (false discovery rate [FDR] < 0.1. Eight genes (ESR1, FSIP2, FRAS1, OSBPL3, EDC4, PALB2, IGFN1, and AGRN were more frequently mutated in mBC as compared to eBC (FDR < 0.01. ESR1 was identified both as a driver and as a metastatic gene (n = 22, odds ratio = 29, 95% CI [9-155], p = 1.2e-12 and also presented with focal amplification (n = 9 for a total of 31 mBCs with either ESR1 mutation or amplification, including 27 hormone receptor positive (HR+ and HER2 negative (HER2- mBCs (19%. HR+/HER2- mBC presented a high prevalence of mutations on genes located on the mechanistic target of rapamycin (mTOR pathway (TSC1 and TSC2 as compared to HR+/HER2- eBC (respectively 6% and 0.7%, p = 0.0004. Other actionable genes were more frequently mutated in HR+ mBC, including ERBB4 (n = 8, NOTCH3 (n = 7, and ALK (n = 7. Analysis of mutational signatures revealed a significant increase in APOBEC-mediated mutagenesis in HR+/HER2- metastatic tumors as compared to primary TCGA samples (p < 2e-16. The main limitations of this study include the absence of bone metastases and the size of the cohort, which might

  19. Procedure for conducting a human-reliability analysis for nuclear power plants. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Bell, B.J.; Swain, A.D.

    1983-05-01

    This document describes in detail a procedure to be followed in conducting a human reliability analysis as part of a probabilistic risk assessment when such an analysis is performed according to the methods described in NUREG/CR-1278, Handbook for Human Reliability Analysis with Emphasis on Nuclear Power Plant Applications. An overview of the procedure describing the major elements of a human reliability analysis is presented along with a detailed description of each element and an example of an actual analysis. An appendix consists of some sample human reliability analysis problems for further study.

  20. Reproducibility of Cognitive Profiles in Psychosis Using Cluster Analysis.

    Science.gov (United States)

    Lewandowski, Kathryn E; Baker, Justin T; McCarthy, Julie M; Norris, Lesley A; Öngür, Dost

    2017-10-18

    Cognitive dysfunction is a core symptom dimension that cuts across the psychoses. Recent findings support classification of patients along the cognitive dimension using cluster analysis; however, data-derived groupings may be highly determined by sampling characteristics and the measures used to derive the clusters, and so their interpretability must be established. We examined cognitive clusters in a cross-diagnostic sample of patients with psychosis and associations with clinical and functional outcomes. We then compared our findings to a previous report of cognitive clusters in a separate sample using a different cognitive battery. Participants with affective or non-affective psychosis (n=120) and healthy controls (n=31) were administered the MATRICS Consensus Cognitive Battery, and clinical and community functioning assessments. Cluster analyses were performed on cognitive variables, and clusters were compared on demographic, cognitive, and clinical measures. Results were compared to findings from our previous report. A four-cluster solution provided a good fit to the data; profiles included a neuropsychologically normal cluster, a globally impaired cluster, and two clusters of mixed profiles. Cognitive burden was associated with symptom severity and poorer community functioning. The patterns of cognitive performance by cluster were highly consistent with our previous findings. We found evidence of four cognitive subgroups of patients with psychosis, with cognitive profiles that map closely to those produced in our previous work. Clusters were associated with clinical and community variables and a measure of premorbid functioning, suggesting that they reflect meaningful groupings: replicable, and related to clinical presentation and functional outcomes. (JINS, 2017, 23, 1-9).

  1. A nonparametric procedure for defining a new humoral immunologic profile in a pilot study on HIV infected patients.

    Directory of Open Access Journals (Sweden)

    Chiara Brombin

    Full Text Available This work aims at identifying a set of humoral immunologic parameters that improve prediction of the activation process in HIV patients. Starting from the well-known impact of humoral immunity in HIV infection, there is still a lack of knowledge in defining the role of the modulation of functional activity and titers of serum antibodies from early stage of infection to the development of AIDS. We propose an integrated approach that combines humoral and clinical parameters in defining the host immunity, implementing algorithms associated with virus control. A number of humoral parameters were simultaneously evaluated in a whole range of serum samples from HIV-positive patients. This issue has been afforded accounting for estimation problems typically related to "feasibility" studies where small sample size in each group and large number of parameters are jointly estimated. We used nonparametric statistical procedures to identify biomarkers in our study which included 42 subjects stratified on five different stages of HIV infection, i.e., Elite Controllers (EC, Long Term Non Progressors (LTNP, HAART, AIDS and Acute Infection (AI. The main goal of the paper is to illustrate a novel profiling method for helping to design a further confirmatory study. A set of seventeen different HIV-specific blood humoral factors were analyzed in all subjects, i.e. IgG and IgA to gp120IIIB, to gp120Bal, to whole gp41, to P1 and T20 gp41 epitopes of the MPER-HR2 region, to QARILAV gp41 epitope of the HR1 region and to CCR5; neutralization activity against five different virus strains and ADCC were also evaluated. Patients were selected on the basis of CD4 cell counts, HIV/RNA and clinical status. The Classification and Regression Trees (CART approach has been used to uncover specific patterns of humoral parameters in different stages of HIV disease. Virus neutralization of primary virus strains and antibodies to gp41 were required to classify patients, suggesting

  2. A nonparametric procedure for defining a new humoral immunologic profile in a pilot study on HIV infected patients.

    Science.gov (United States)

    Brombin, Chiara; Diomede, Lorenzo; Tudor, Daniela; Drillet, Anne Sophie; Pastori, Claudia; Poli, Elena; Riva, Agostino; Uberti-Foppa, Caterina; Galli, Massimo; Di Serio, Clelia; Bomsel, Morgane; Lopalco, Lucia

    2013-01-01

    This work aims at identifying a set of humoral immunologic parameters that improve prediction of the activation process in HIV patients. Starting from the well-known impact of humoral immunity in HIV infection, there is still a lack of knowledge in defining the role of the modulation of functional activity and titers of serum antibodies from early stage of infection to the development of AIDS. We propose an integrated approach that combines humoral and clinical parameters in defining the host immunity, implementing algorithms associated with virus control. A number of humoral parameters were simultaneously evaluated in a whole range of serum samples from HIV-positive patients. This issue has been afforded accounting for estimation problems typically related to "feasibility" studies where small sample size in each group and large number of parameters are jointly estimated. We used nonparametric statistical procedures to identify biomarkers in our study which included 42 subjects stratified on five different stages of HIV infection, i.e., Elite Controllers (EC), Long Term Non Progressors (LTNP), HAART, AIDS and Acute Infection (AI). The main goal of the paper is to illustrate a novel profiling method for helping to design a further confirmatory study. A set of seventeen different HIV-specific blood humoral factors were analyzed in all subjects, i.e. IgG and IgA to gp120IIIB, to gp120Bal, to whole gp41, to P1 and T20 gp41 epitopes of the MPER-HR2 region, to QARILAV gp41 epitope of the HR1 region and to CCR5; neutralization activity against five different virus strains and ADCC were also evaluated. Patients were selected on the basis of CD4 cell counts, HIV/RNA and clinical status. The Classification and Regression Trees (CART) approach has been used to uncover specific patterns of humoral parameters in different stages of HIV disease. Virus neutralization of primary virus strains and antibodies to gp41 were required to classify patients, suggesting that clinical

  3. PROCEDURE FOR ANALYSIS AND EVALUATION OF MARKET POSITION PRODUCTION ORGANIZATION

    Directory of Open Access Journals (Sweden)

    A. N. Polozova

    2014-01-01

    Full Text Available Summary. Methodical procedures economic monitoring market position of industrial organization, particularly those relating to food production, including the 5 elements: matrix «component of business processes», matrix «materiality – efficiency», matrix «materiality – relevant», matrix emption and hindering factors matrix operation scenarios. Substantiated components assess the strengths and weaknesses of the business activities of organizations that characterize the state of internal business environment on the elements: production, organization, personnel, finance, marketing. The advantages of the matrix «materiality – relevance» consisting of 2 materiality level - high and low, and 3 directions relevance – «no change», «gain importance in the future», «lose importance in the future». Presented the contents of the matrix «scenarios functioning of the organization», involving 6 attribute levels, 10 classes of scenarios, 19 activities, including an optimistic and pessimistic. The evaluation of primary classes of scenarios, characterized by the properties of «development», «dynamic equilibrium», «quality improvement», «competitiveness», «favorable realization of opportunities», «competition resistance».

  4. Analysis of generalized Schwarz alternating procedure for domain decomposition

    Energy Technology Data Exchange (ETDEWEB)

    Engquist, B.; Zhao, Hongkai [Univ. of California, Los Angeles, CA (United States)

    1996-12-31

    The Schwartz alternating method(SAM) is the theoretical basis for domain decomposition which itself is a powerful tool both for parallel computation and for computing in complicated domains. The convergence rate of the classical SAM is very sensitive to the overlapping size between each subdomain, which is not desirable for most applications. We propose a generalized SAM procedure which is an extension of the modified SAM proposed by P.-L. Lions. Instead of using only Dirichlet data at the artificial boundary between subdomains, we take a convex combination of u and {partial_derivative}u/{partial_derivative}n, i.e. {partial_derivative}u/{partial_derivative}n + {Lambda}u, where {Lambda} is some {open_quotes}positive{close_quotes} operator. Convergence of the modified SAM without overlapping in a quite general setting has been proven by P.-L.Lions using delicate energy estimates. The important questions remain for the generalized SAM. (1) What is the most essential mechanism for convergence without overlapping? (2) Given the partial differential equation, what is the best choice for the positive operator {Lambda}? (3) In the overlapping case, is the generalized SAM superior to the classical SAM? (4) What is the convergence rate and what does it depend on? (5) Numerically can we obtain an easy to implement operator {Lambda} such that the convergence is independent of the mesh size. To analyze the convergence of the generalized SAM we focus, for simplicity, on the Poisson equation for two typical geometry in two subdomain case.

  5. Iranian Voice Quality of Life Profile (IVQLP): Factor Analysis.

    Science.gov (United States)

    Dehqan, Ali; Yadegari, Fariba; Scherer, Ronald C; Asgari, Ali; Dabirmoghadam, Payman

    2017-09-01

    An important domain in health-related quality of life evaluations is quality of life perceptions due to having a voice disorder. The objective of this study was to examine the factor structure of the Iranian Voice Quality of Life Profile (IVQLP) based on Exploratory Factor Analysis and confirmatory factor analysis. The study sample consisted of 280 patients (174 males and 106 females) diagnosed with MTD, benign organic disorders such as polyps and nodules, and unilateral vocal fold paralysis and cancer. To evaluate the different dimensions of the IVQLP, a principal component analysis (PCA) was conducted. Confirmatory factor analysis (CFA) was used to investigate the fitting of extracted dimensions and construct validity of the IVQLP. The results showed that the IVQLP has a 4-factor structure. The first factor has 17 items and refers to Emotions. The second factor has 12 items and refers to Individual/Social Relations. The third factor with 6 items refers to Occupation, and the forth with 5 items relates to Psychosomatic characteristics. The conclusion of this study is that the concept of quality of life in Iranian patients with voice disorders is somewhat different from that of Western patients. This difference can be seen in. the large number of items related to an Emotional factor and the identification of a Psychosomatic factor. Copyright © 2017 The Voice Foundation. All rights reserved.

  6. A Bayesian multidimensional scaling procedure for the spatial analysis of revealed choice data

    NARCIS (Netherlands)

    DeSarbo, WS; Kim, Y; Fong, D

    1999-01-01

    We present a new Bayesian formulation of a vector multidimensional scaling procedure for the spatial analysis of binary choice data. The Gibbs sampler is gainfully employed to estimate the posterior distribution of the specified scalar products, bilinear model parameters. The computational procedure

  7. Responding to Self-Harm: A Documentary Analysis of Agency Policy and Procedure

    Science.gov (United States)

    Paul, Sally; Hill, Malcolm

    2013-01-01

    This paper reports on the findings of a documentary analysis of policies and procedures relating to self-harm from a range of organisations working with young people in the UK. It identifies the extent to which policies and/or procedures relating to self-harm are available for service providers and offers a wider understanding of the concepts of…

  8. Procedures for analysis of debris relative to Space Shuttle systems

    Science.gov (United States)

    Kim, Hae Soo; Cummings, Virginia J.

    1993-01-01

    Debris samples collected from various Space Shuttle systems have been submitted to the Microchemical Analysis Branch. This investigation was initiated to develop optimal techniques for the analysis of debris. Optical microscopy provides information about the morphology and size of crystallites, particle sizes, amorphous phases, glass phases, and poorly crystallized materials. Scanning electron microscopy with energy dispersive spectrometry is utilized for information on surface morphology and qualitative elemental content of debris. Analytical electron microscopy with wavelength dispersive spectrometry provides information on the quantitative elemental content of debris.

  9. Free choice profiling sensory analysis to discriminate coffees

    Directory of Open Access Journals (Sweden)

    Cíntia Sorane Good Kitzberger

    2016-12-01

    Full Text Available Sensory attributes were evaluated from Arabica coffee genotypes growing in two places in Brazil, Mandaguari and Londrina. Post-harvest and roasted process was standardized. Free choice profiling sensory analysis was apply to investigate the influence of genetic variability and local cultivation (Londrina and Mandaguari, Brazil on the sensory characteristics of coffee genotypes. A sensory panel evaluated coffees from Mandaguari in two groups: one (Sarchimor derived, IPR100, IPR102, IPR105, IPR106 characterized by transparency, coffee colour, green aroma, taste (green, bitter, fermented, astringent and a watery texture, another group (Catuaí, Sarchimor derived, IPR101, IPR103 was characterized by coffee colour, brightness, aroma (coffee, acid, sweet, chocolate, acidity, bitterness, burnt aroma, sweetness and full-bodied. Coffees from Londrina presented brightness, coffee colour, sweet, green, burnt aroma, astringent, bitter, fermented, green taste; and watery texture (Catuaí, IPR97, IPR98, IPR100. Another group (Sarchimor derived, IPR101, IPR102, IPR103, IPR105, IPR106 were associated with turbidity, aroma (green, coffee, sweet, acidity, astringency, bitterness, sweetness and full-bodied. Catuaí, Iapar59, IPR99, IPR101, IPR103 and IPR108 exhibited positive attributes when grown in either locale. Edaphoclimatic conditions play a major role in the sensory profiles of coffee.

  10. Conducting On-Farm Animal Research: Procedures & Economic Analysis.

    Science.gov (United States)

    Amir, Pervaiz; Knipscheer, Hendrik C.

    This book is intended to give animal scientists elementary tools to perform on-farm livestock analysis and to provide crop-oriented farming systems researchers with methods for conducting animal research. Chapter 1 describes farming systems research as a systems approach to on-farm animal research. Chapter 2 outlines some important…

  11. Comparison of Three Criteria for Discriminant Analysis Procedure ...

    African Journals Online (AJOL)

    2012-08-01

    Aug 1, 2012 ... Department of Mathematics and Statistics. Federal ... Introduction: Discriminant Analysis is concerned with the problem of classification. This problem of classification arises when an investigator makes a number of measurements on an individual and wishes ... costs of assignment, the a priori probabilities.

  12. PROC LCA: A SAS Procedure for Latent Class Analysis

    Science.gov (United States)

    Lanza, Stephanie T.; Collins, Linda M.; Lemmon, David R.; Schafer, Joseph L.

    2007-01-01

    Latent class analysis (LCA) is a statistical method used to identify a set of discrete, mutually exclusive latent classes of individuals based on their responses to a set of observed categorical variables. In multiple-group LCA, both the measurement part and structural part of the model can vary across groups, and measurement invariance across…

  13. Photogrammetric analysis of facial profile in Persian adults.

    Science.gov (United States)

    Jamilian, Abdolreza; Darnahal, Alireza; Hamedi, Roya; Kamali, Zinat; Toopchi, Shabnam

    2016-01-01

    When facial photographs are analyzed for the purpose of identifying esthetics norms, differences in dentofacial relationships among ethnic and racial groups must be taken into consideration. Therefore, it is necessary to establish standards for various populations. The purpose of this study was to establish norms of photogrammetric soft tissue profile analysis for Persian adults. Pretreatment lateral photographs of 147 subjects (66 men and 81 women aged 18-35 years) with a Class I skeletal pattern were collected. Twelve angular parameters were evaluated in the subjects. Values for men and women were compared with a t test. Statistically significant sexual dimorphism was found for nasofrontal (P < 0.059), nasal (P < 0.059), nasal dorsum (P < 0.001), cervicomental (P < 0.001), facial convexity (P < 0.004), and total facial convexity (P < 0.002) angles. The mean values obtained from this study can assist in orthodontic diagnosis and treatment planning for Persian adults.

  14. Cost-effectiveness analysis for clinical procedures in oncology.

    Science.gov (United States)

    Weinstein, M C

    1980-01-01

    The provision of medical care consumes resources, and the resources available for the provision of medical care are limited. Decisions are being made at many levels of the Health Care System, including providers and fiscal intermediaries, to allocate these resources. Such decisions, however, are often inconsistent with the objective of deriving the maximum health benefits from the resources spent. Many cost-effectiveness and benefit-cost studies have been conducted in order to guide present and future resource allocation decisions. Many analyses have not been accepted by health care decision makers because a critical factor or issue has been omitted. In the attempt to be objective, the analyst may avoid uncertainties or subjective value judgments that often dominate the thinking of the decision maker. The role of the analyst in cost-effectiveness analysis, as in decision analysis for the individual patient, is to clarify and highlight such factors not to obfuscate them.

  15. Microarray analysis of gene expression profiles in ripening pineapple fruits

    Directory of Open Access Journals (Sweden)

    Koia Jonni H

    2012-12-01

    Full Text Available Abstract Background Pineapple (Ananas comosus is a tropical fruit crop of significant commercial importance. Although the physiological changes that occur during pineapple fruit development have been well characterized, little is known about the molecular events that occur during the fruit ripening process. Understanding the molecular basis of pineapple fruit ripening will aid the development of new varieties via molecular breeding or genetic modification. In this study we developed a 9277 element pineapple microarray and used it to profile gene expression changes that occur during pineapple fruit ripening. Results Microarray analyses identified 271 unique cDNAs differentially expressed at least 1.5-fold between the mature green and mature yellow stages of pineapple fruit ripening. Among these 271 sequences, 184 share significant homology with genes encoding proteins of known function, 53 share homology with genes encoding proteins of unknown function and 34 share no significant homology with any database accession. Of the 237 pineapple sequences with homologs, 160 were up-regulated and 77 were down-regulated during pineapple fruit ripening. DAVID Functional Annotation Cluster (FAC analysis of all 237 sequences with homologs revealed confident enrichment scores for redox activity, organic acid metabolism, metalloenzyme activity, glycolysis, vitamin C biosynthesis, antioxidant activity and cysteine peptidase activity, indicating the functional significance and importance of these processes and pathways during pineapple fruit development. Quantitative real-time PCR analysis validated the microarray expression results for nine out of ten genes tested. Conclusions This is the first report of a microarray based gene expression study undertaken in pineapple. Our bioinformatic analyses of the transcript profiles have identified a number of genes, processes and pathways with putative involvement in the pineapple fruit ripening process. This study

  16. Microarray analysis of gene expression profiles in ripening pineapple fruits.

    Science.gov (United States)

    Koia, Jonni H; Moyle, Richard L; Botella, Jose R

    2012-12-18

    Pineapple (Ananas comosus) is a tropical fruit crop of significant commercial importance. Although the physiological changes that occur during pineapple fruit development have been well characterized, little is known about the molecular events that occur during the fruit ripening process. Understanding the molecular basis of pineapple fruit ripening will aid the development of new varieties via molecular breeding or genetic modification. In this study we developed a 9277 element pineapple microarray and used it to profile gene expression changes that occur during pineapple fruit ripening. Microarray analyses identified 271 unique cDNAs differentially expressed at least 1.5-fold between the mature green and mature yellow stages of pineapple fruit ripening. Among these 271 sequences, 184 share significant homology with genes encoding proteins of known function, 53 share homology with genes encoding proteins of unknown function and 34 share no significant homology with any database accession. Of the 237 pineapple sequences with homologs, 160 were up-regulated and 77 were down-regulated during pineapple fruit ripening. DAVID Functional Annotation Cluster (FAC) analysis of all 237 sequences with homologs revealed confident enrichment scores for redox activity, organic acid metabolism, metalloenzyme activity, glycolysis, vitamin C biosynthesis, antioxidant activity and cysteine peptidase activity, indicating the functional significance and importance of these processes and pathways during pineapple fruit development. Quantitative real-time PCR analysis validated the microarray expression results for nine out of ten genes tested. This is the first report of a microarray based gene expression study undertaken in pineapple. Our bioinformatic analyses of the transcript profiles have identified a number of genes, processes and pathways with putative involvement in the pineapple fruit ripening process. This study extends our knowledge of the molecular basis of pineapple fruit

  17. The Impact of Patient Profiles and Procedures on Hospitalization Costs through Length of Stay in Community-Acquired Pneumonia Patients Based on a Japanese Administrative Database.

    Directory of Open Access Journals (Sweden)

    Hironori Uematsu

    Full Text Available Community-acquired pneumonia is a common cause of patient hospitalization, and its burden on health care systems is increasing in aging societies. In this study, we aimed to investigate the factors that affect hospitalization costs in community-acquired pneumonia patients while considering the intermediate influence of patient length of stay.Using a multi-institutional administrative claims database, we analyzed 30,041 patients hospitalized for community-acquired pneumonia who had been discharged between April 1, 2012 and September 30, 2013 from 289 acute care hospitals in Japan. Possible factors associated with hospitalization costs were investigated using structural equation modeling with length of stay as an intermediate variable. We calculated the direct, indirect (through length of stay, and total effects of the candidate factors on hospitalization costs in the model. Lastly, we calculated the ratio of indirect effects to direct effects for each factor.The structural equation model showed that higher disease severities (using A-DROP, Barthel Index, and Charlson Comorbidity Index scores, use of mechanical ventilation, and tube feeding were associated with higher hospitalization costs, regardless of the intermediate influence of length of stay. The severity factors were also associated with longer length of stay durations. The ratio of indirect effects to direct effects on total hospitalization costs showed that the former was greater than the latter in the factors, except in the use of mechanical ventilation.Our structural equation modeling analysis indicated that patient profiles and procedures impacted on hospitalization costs both directly and indirectly. Furthermore, the profiles were generally shown to have greater indirect effects (through length of stay on hospitalization costs than direct effects. These findings may be useful in supporting the more appropriate distribution of health care resources.

  18. Cochlear Hydrops Analysis Masking Procedure Results in Patients With Unilateral Meniere's Disease

    NARCIS (Netherlands)

    Kingma, Charlotte M.; Wit, Hero P.

    Objective: To investigate the usefulness of the cochlear hydrops analysis masking procedure (CHAMP) as an additional diagnostic test in patients with definite unilateral Meniere's disease. Study Design: Prospective validation study for a diagnostic test. Setting: Tertiary referral center. Patients:

  19. 14 CFR Appendix I to Part 417 - Methodologies for Toxic Release Hazard Analysis and Operational Procedures

    Science.gov (United States)

    2010-01-01

    ... either the methodology provided in the Risk Management Plan (RMP) Offsite Consequence Analysis Guidance..., App. I Appendix I to Part 417—Methodologies for Toxic Release Hazard Analysis and Operational Procedures I417.1General This appendix provides methodologies for performing toxic release hazard analysis...

  20. Fundamental Parameter Method Applied to X-Ray Line Profile Analysis

    Directory of Open Access Journals (Sweden)

    Florica Matei

    2016-11-01

    Full Text Available Crystallite size strain effect the mechanical, electric, magnetic and optical properties of many kind of the nanomaterials. The effects of the finite crystallite size and lattice strain can be very well observed as the deformation in the shape of the X-ray line profile (XRLP. In this contribution we have used the fundamental parameter (FP [1,2] method to evaluate the nanostructure materials assuming a theoretical model of experimental XRLP.  In this contribution we have used various distribution functions such as normal, lognormal, Gumbel, Maxwell and Student. The best values of the crystallite size of nanostructued materials are chosen by analysis of root mean squares of residuals and by correlation matrix of the fit parameters. The entire procedure was implemented in the GnuPlot script.

  1. Analysis of the thermal profiles and the charcoal gravimetric yield in three variations of rectangular brick

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Rogerio Lima Mota de; Alves Junior, Edson; Mulina, Bruno Henrique Oliveira; Borges, Valerio Luiz; Carvalho, Solidonio Rodrigues de [Federal University of Uberlandia - UFU, MG (Brazil). School of Mechanical Engineering - FEMEC], e-mails: rogerio@mecanica.ufu.br, edson@mec.ufu.br, vlborges@mecanica.ufu.br, srcarvalho@mecanica.ufu.br

    2010-07-01

    Charcoal assumes a major role in Brazilian economic scenario. The procedure for obtaining charcoal consists in carbonization of wood at certain specific temperatures in kilns. This ancient process has a few joined technologies and the kilns for such practice do not have any control instruments, in their great majority, becoming dependent on the ability of its operators. However, in recent decades several studies have been developed to improve the practice as well as the equipment that involve and control the stages of charcoal production. In this sense, this work proposes the analysis of the thermal profiles and the gravimetric yield in three variations of a rectangular brick kiln called RAC220: traditional (without any type of instrumentation), instrumented with thermal sensors (RTD PT100) and adapted with gasifier. The goal is to correlate temperature, gravimetric yield and quality of the produced charcoal. Immediate analyses were performed to determine the amount of fixed carbon, volatile gases and ashes contents in charcoal. Through such measurement procedures, together with statistical analysis, the aim is to identify an important tool to reduce the time of charcoal production and also contributes to minimize losses and to increase the thermal efficiency of the production process. (author)

  2. An outcomes analysis of 2142 breast reduction procedures.

    Science.gov (United States)

    Manahan, Michele Ann; Buretta, Kate J; Chang, David; Mithani, Suhail K; Mallalieu, Jesse; Shermak, Michele A

    2015-03-01

    Breast reduction alleviates macromastia symptoms and facilitates symmetrical breast reconstruction after cancer treatment. We investigated a large series of consecutive breast reductions to study important factors that impact outcomes. An institutional review board-approved, retrospective review of all breast reductions from 1999 to 2009 in a single institution was performed using the medical record for demographics, medical history, physical examination, intraoperative data, and postoperative complications. Multivariate statistical analysis was performed using Stata 1.0. P ≤ 0.05 defined significance. Seventeen surgeons performed 2152 consecutive breast reductions on 1148 patients using inferior pedicle/Wise pattern (56.4%), medial pedicle/Wise pattern (16.8%), superior pedicle/nipple graft/Wise pattern (15.1%), superior pedicle/vertical pattern (11.6%), and liposuction (0.1%) techniques. Complications included discernible scars (14.5%), nonsurgical wounds (13.5%), fat necrosis (8.2%), infection (7.3%), wounds requiring negative pressure wound therapy or reoperation (1.4%), and seroma (1.2%). Reoperation rates were 6.7% for scars, 1.4% for fat necrosis, and 1% for wounds.Body mass index greater than or equal to 35 kg/m increased risk of infections [odds ratio (OR), 2.3, P = 0.000], seromas (OR, 2.9, P = 0.03), fat necrosis (OR, 2.0, P = 0.002), and minor wounds (OR, 1.7, P = 0.001). Cardiac disease increased reoperation for scar (OR, 3.0, P = 0.04) and fat necrosis (OR, 5.3, P = 0.03). Tobacco use increased infection rate (OR, 2.1, P = 0.008). Secondary surgery increased seromas (OR, 12.0, P = 0.001). Previous hysterectomy/oophorectomy increased risk of wound reoperations (OR, 3.4, P = 0.02), and exogenous hormone supplementation trended toward decreasing infections (OR, 0.5, P = 0.08). χ analysis revealed 7.8% infection risk without exogenous hormone versus 3.8% risk with hormone supplementation (P = 0.02). Morbid obesity, tobacco, cardiac history, and

  3. A multi-model analysis of vertical ozone profiles

    Directory of Open Access Journals (Sweden)

    J. E. Jonson

    2010-06-01

    Full Text Available A multi-model study of the long-range transport of ozone and its precursors from major anthropogenic source regions was coordinated by the Task Force on Hemispheric Transport of Air Pollution (TF HTAP under the Convention on Long-range Transboundary Air Pollution (LRTAP. Vertical profiles of ozone at 12-h intervals from 2001 are available from twelve of the models contributing to this study and are compared here with observed profiles from ozonesondes. The contributions from each major source region are analysed for selected sondes, and this analysis is supplemented by retroplume calculations using the FLEXPART Lagrangian particle dispersion model to provide insight into the origin of ozone transport events and the cause of differences between the models and observations.

    In the boundary layer ozone levels are in general strongly affected by regional sources and sinks. With a considerably longer lifetime in the free troposphere, ozone here is to a much larger extent affected by processes on a larger scale such as intercontinental transport and exchange with the stratosphere. Such individual events are difficult to trace over several days or weeks of transport. This may explain why statistical relationships between models and ozonesonde measurements are far less satisfactory than shown in previous studies for surface measurements at all seasons. The lowest bias between model-calculated ozone profiles and the ozonesonde measurements is seen in the winter and autumn months. Following the increase in photochemical activity in the spring and summer months, the spread in model results increases, and the agreement between ozonesonde measurements and the individual models deteriorates further.

    At selected sites calculated contributions to ozone levels in the free troposphere from intercontinental transport are shown. Intercontinental transport is identified based on differences in model calculations with unperturbed emissions and

  4. A multi-model analysis of vertical ozone profiles

    Science.gov (United States)

    Jonson, J. E.; Stohl, A.; Fiore, A. M.; Hess, P.; Szopa, S.; Wild, O.; Zeng, G.; Dentener, F. J.; Lupu, A.; Schultz, M. G.; Duncan, B. N.; Sudo, K.; Wind, P.; Schulz, M.; Marmer, E.; Cuvelier, C.; Keating, T.; Zuber, A.; Valdebenito, A.; Dorokhov, V.; de Backer, H.; Davies, J.; Chen, G. H.; Johnson, B.; Tarasick, D. W.; Stübi, R.; Newchurch, M. J.; von der Gathen, P.; Steinbrecht, W.; Claude, H.

    2010-06-01

    A multi-model study of the long-range transport of ozone and its precursors from major anthropogenic source regions was coordinated by the Task Force on Hemispheric Transport of Air Pollution (TF HTAP) under the Convention on Long-range Transboundary Air Pollution (LRTAP). Vertical profiles of ozone at 12-h intervals from 2001 are available from twelve of the models contributing to this study and are compared here with observed profiles from ozonesondes. The contributions from each major source region are analysed for selected sondes, and this analysis is supplemented by retroplume calculations using the FLEXPART Lagrangian particle dispersion model to provide insight into the origin of ozone transport events and the cause of differences between the models and observations. In the boundary layer ozone levels are in general strongly affected by regional sources and sinks. With a considerably longer lifetime in the free troposphere, ozone here is to a much larger extent affected by processes on a larger scale such as intercontinental transport and exchange with the stratosphere. Such individual events are difficult to trace over several days or weeks of transport. This may explain why statistical relationships between models and ozonesonde measurements are far less satisfactory than shown in previous studies for surface measurements at all seasons. The lowest bias between model-calculated ozone profiles and the ozonesonde measurements is seen in the winter and autumn months. Following the increase in photochemical activity in the spring and summer months, the spread in model results increases, and the agreement between ozonesonde measurements and the individual models deteriorates further. At selected sites calculated contributions to ozone levels in the free troposphere from intercontinental transport are shown. Intercontinental transport is identified based on differences in model calculations with unperturbed emissions and emissions reduced by 20% by region

  5. Distinguishing subpopulations of marijuana users with latent profile analysis.

    Science.gov (United States)

    Pearson, Matthew R; Bravo, Adrian J; Conner, Bradley T

    2017-03-01

    Although marijuana is the most commonly used illicit drug in the United States, little is known about the effects of typical marijuana use patterns and whether there are distinct subgroups of marijuana users. The present study used latent profile analysis to determine the number of distinct subgroups of marijuana users in a large sample of college students (n=2129 past month marijuana users across 11 universities). We also examined how these distinct groups differ on several putative risk/protective factors (e.g., personality traits, perceptions of marijuana, and motives for using marijuana). Using the Lo-Mendell-Rubin Likelihood Ratio Test, we identified four latent classes with the largest class consisting of infrequent marijuana users, and three other classes demonstrating increasingly frequent use and more negative consequences with the most severe class being the smallest class. We found the largest between-class differences (i.e., distinctions across classes) to be on identification with being a marijuana user and use of protective behavioral strategies (PBS), such that the heavier user classes showed higher identification with marijuana users and lower use of PBS. Our findings demonstrate that college student marijuana users are a heterogeneous group with different profiles of risk/protective factors and that those who use marijuana a few times per month are different from those who are near-daily or daily users. Our findings also serve as a call to action for the field to consider examining identification with being a marijuana user and the use of PBS in future marijuana studies. Copyright © 2016. Published by Elsevier B.V.

  6. Gene expression profiling and bioinformatics analysis of gastric carcinoma.

    Science.gov (United States)

    Liu, Ningning; Liu, Xuan; Zhou, Ning; Wu, Qiong; Zhou, Lihong; Li, Qi

    2014-06-01

    Gastric cancer remains one of the major health problems worldwide, and it is one of the most common cancers and the leading cause of cancer-related deaths in China. This study was to analyze the expression profiles of genes in gastric carcinoma, and predict potential regulating factors. The gene expression profile data GSE13911 was downloaded from Gene Expression Omnibus and the differentially expressed genes (DEGs) were identified by t-test. Gene modules were constructed using hierarchical clustering in R based on average linkage and Pearson's correlation coefficient and functional analysis for these genes were performed with DAVID. Genes in each module with Pearson's correlation coefficient >0.3 were obtained to construct co-expression network. Protein-protein interactions (PPIs) were identified by comparing protein-protein interaction (PPI) network with co-expression networks. In addition, the potential regulatory microRNAs and the transcription factors for each module were screened out. In this study, six modules associated with protein degradation, cell cycle, protein trafficking and immunoreaction were identified. COPS5 (COP9 Subunit 5) was the core protein in the largest PPI network of module 1. The transcription factors MYC and MAZ (Myc-associated zinc-finger protein) were enriched in module 1. A total of 9 microRNA-target bi-clusters were identified and module 1 enriched 20 genes targeting to miR-17-92 gene cluster(miR-17/20ab)and miR-106b-25 gene cluster (miR-106b/93). In conclusion, we constructed 6 gene modules and screened out some genes, transcriptional factors and microRNAs that may be used as potential molecular biomarkers for gastric carcinoma. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. Chlorhexidine application in adhesive procedures: a meta-regression analysis.

    Science.gov (United States)

    Collares, Fabrício Mezzomo; Rodrigues, Stéfani Becker; Leitune, Vicente Castelo; Celeste, Roger Keller; Borba de Araújo, Fernando; Samuel, Susana Maria

    2013-02-01

    To evaluate the association between chlorhexidine application and the bond strength of an adhesive system to dentin and to assess the association among eight other variables. Laboratory studies evaluating the use of chlorhexidine on dentin that verified the immediate and longitudinal bond strength were included. The terms were selected according to the Medical Subject Headings (MeSH) for PubMed and adapted for the other databases accordingly. Systematic searches were conducted in 3 electronic databases in December 2010: PubMed, EMBASE, and LILACS. Languages were limited to English, Spanish, and Portuguese. All longitudinal bond strength studies were analyzed in accordance with the inclusion/exclusion criteria, totaling 16 articles. A meta-regression analysis was performed with articles containing complete mean bond strength data (n = 14 articles). Our results showed that the association between the concentration of chlorhexidine and the bond strength is, apparently, not linear. Therefore, future large-scale studies should be developed to investigate the association between the chlorhexidine concentration and hybrid layer preservation.

  8. A Typology of Child School Behavior: Investigation Using Latent Profile Analysis and Cluster Analysis

    Science.gov (United States)

    Mindrila, Diana L.

    2016-01-01

    To describe and facilitate the identification of child school behavior patterns, we developed a typology of child school behavior (ages 6-11 years) using the norming data (N = 2,338) for the second edition of the Behavior Assessment System for Children Teacher Rating-Child form). Latent profile analysis was conducted with the entire data set,…

  9. Exploratory factor analysis of the Oral Health Impact Profile.

    Science.gov (United States)

    John, M T; Reissmann, D R; Feuerstahler, L; Waller, N; Baba, K; Larsson, P; Celebić, A; Szabo, G; Rener-Sitar, K

    2014-09-01

    Although oral health-related quality of life (OHRQoL) as measured by the Oral Health Impact Profile (OHIP) is thought to be multidimensional, the nature of these dimensions is not known. The aim of this report was to explore the dimensionality of the OHIP using the Dimensions of OHRQoL (DOQ) Project, an international study of general population subjects and prosthodontic patients. Using the project's Learning Sample (n = 5173), we conducted an exploratory factor analysis on the 46 OHIP items not specifically referring to dentures for 5146 subjects with sufficiently complete data. The first eigenvalue (27·0) of the polychoric correlation matrix was more than ten times larger than the second eigenvalue (2·6), suggesting the presence of a dominant, higher-order general factor. Follow-up analyses with Horn's parallel analysis revealed a viable second-order, four-factor solution. An oblique rotation of this solution revealed four highly correlated factors that we named Oral Function, Oro-facial Pain, Oro-facial Appearance and Psychosocial Impact. These four dimensions and the strong general factor are two viable hypotheses for the factor structure of the OHIP. © 2014 John Wiley & Sons Ltd.

  10. Volcanic Ash and SO2 retrievals using synthetic MODIS TIR data: comparison between inversion procedures and sensitivity analysis

    Directory of Open Access Journals (Sweden)

    Stefano Corradini

    2015-02-01

    Full Text Available In this work the volcanic ash and SO2 retrievals obtained by applying three different procedures (LUT - Look Up Table, NN - Neural Network and VPR - Volcanic Plume Removal on MODIS Thermal InfraRed (TIR synthetic measurements have been compared. The synthetic measurements are generated using MODTRAN Radiative Transfer Model (RTM for defined volcanic cloud configurations. The results, presented as the percentage difference between the retrieved ash and SO2 total masses and the true values used for the synthetic data generation, indicate maximum differences of +/- 15% and +/- 10% for all the procedures and for ash and SO2 retrievals respectively. A sensitivity analysis has been also realized to investigate the influence of volcanic cloud altitude and water vapour profile on SO2 retrievals at 7.3 and 8.6 μm. Results confirm the high sensitivity of the 7.3 μm retrieval to the volcanic cloud altitude and show that the SO2 total masses estimated at 7.3 and 8.6 μm separately can be used to improve the information on the plume height. Finally, the water vapour profile is used to compute the minimum altitude over which the 7.3 μm retrieval is effective. 

  11. A combined analysis of genome-wide expression profiling of bipolar disorder in human prefrontal cortex.

    Science.gov (United States)

    Wang, Jinglu; Qu, Susu; Wang, Weixiao; Guo, Liyuan; Zhang, Kunlin; Chang, Suhua; Wang, Jing

    2016-11-01

    Numbers of gene expression profiling studies of bipolar disorder have been published. Besides different array chips and tissues, variety of the data processes in different cohorts aggravated the inconsistency of results of these genome-wide gene expression profiling studies. By searching the gene expression databases, we obtained six data sets for prefrontal cortex (PFC) of bipolar disorder with raw data and combinable platforms. We used standardized pre-processing and quality control procedures to analyze each data set separately and then combined them into a large gene expression matrix with 101 bipolar disorder subjects and 106 controls. A standard linear mixed-effects model was used to calculate the differentially expressed genes (DEGs). Multiple levels of sensitivity analyses and cross validation with genetic data were conducted. Functional and network analyses were carried out on basis of the DEGs. In the result, we identified 198 unique differentially expressed genes in the PFC of bipolar disorder and control. Among them, 115 DEGs were robust to at least three leave-one-out tests or different pre-processing methods; 51 DEGs were validated with genetic association signals. Pathway enrichment analysis showed these DEGs were related with regulation of neurological system, cell death and apoptosis, and several basic binding processes. Protein-protein interaction network further identified one key hub gene. We have contributed the most comprehensive integrated analysis of bipolar disorder expression profiling studies in PFC to date. The DEGs, especially those with multiple validations, may denote a common signature of bipolar disorder and contribute to the pathogenesis of disease. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. The Tactual Profile: Development of a procedure to assess the tactual functioning of children who are blind

    NARCIS (Netherlands)

    Withagen, A.J.; Vervloed, M.P.J.; Janssen, N.M.; Knoors, H.E.T.; Verhoeven, L.T.W.

    2009-01-01

    The Tactual Profile assesses tactual functioning of children with severe visual impairments between 0 and 16 years of age. The Tactual Profile consists of 430 items, measuring tactile skills required for performing everyday tasks at home and in school. Items are graded according to age level and

  13. The Tactual Profile: Development of a Procedure to Assess the Tactual Functioning of Children Who Are Blind

    Science.gov (United States)

    Withagen, Ans; Vervloed, Mathijs P. J.; Janssen, Neeltje M.; Knoors, Harry; Verhoeven, Ludo

    2009-01-01

    The Tactual Profile assesses tactual functioning of children with severe visual impairments between 0 and 16 years of age. The Tactual Profile consists of 430 items, measuring tactile skills required for performing everyday tasks at home and in school. Items are graded according to age level and divided into three domains: tactual sensory, tactual…

  14. Analysis of half diallel mating designs I: a practical analysis procedure for ANOVA approximation.

    Science.gov (United States)

    G.R. Johnson; J.N. King

    1998-01-01

    Procedures to analyze half-diallel mating designs using the SAS statistical package are presented. The procedure requires two runs of PROC and VARCOMP and results in estimates of additive and non-additive genetic variation. The procedures described can be modified to work on most statistical software packages which can compute variance component estimates. The...

  15. Studying variations in the PCDD/PCDF profile across various food products using multivariate statistical analysis

    Energy Technology Data Exchange (ETDEWEB)

    Antignac, Jean-Philippe [Ecole Nationale Veterinaire de Nantes (ENVN), Laboratoire d' Etude des Residus et Contaminants dans les Aliments (LABERCA), Nantes (France); LABERCA-ENVN, Nantes (France); Marchand, Philippe; Gade, Christel; Matayron, Gilles; Bizec, Bruno Le; Andre, Francois [Ecole Nationale Veterinaire de Nantes (ENVN), Laboratoire d' Etude des Residus et Contaminants dans les Aliments (LABERCA), Nantes (France); Qannari, El Mostafa [Ecole Nationale d' Ingenieurs des Techniques des Industries Agricoles et Alimentaires (ENITIAA), Unite de Sensometrie et de Chimiometrie, La Geraudiere, Nantes (France)

    2006-01-01

    Polychlorinated dibenzo-p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF) are widely recognized by the scientific community as persistent organic pollutants due to their toxicity and adverse effects on wildlife and human health. The actual regulation dedicated to the monitoring of dioxins in food is based on the measurement of 17 congener concentrations. The final result is reported as a toxic equivalent value that takes into account the relative toxicity of each congener. This procedure can minimize the qualitative information available from the abundances of each PCDD/PCDF congener: the characteristic contamination profile of the sample. Multivariate statistical techniques, such as principal component analysis (PCA) or linear discriminant analysis (LDA), represent an interesting way to investigate this qualitative information. Nevertheless, they have only been applied to the analysis of contamination data from food products and biological matrices infrequently. The objective of the present study was to analyze a large data set from dioxin analyses performed on various food products of animal origin. The results demonstrate the existence of differences in congener-specific patterns between the analyzed samples. Variability was first demonstrated in terms of the food type (fish, meat, milk, fatty products). Then a variability was observed that was related to the specific animal species for meat and milk samples (bovine, ovine, porcine, caprine and poultry). Some practical applications of these results are discussed. The origin(s) of the observed differences, as well as their significance, now remain to be investigated, both in terms of environmental factors and transfer through living organisms. A better knowledge of the relation between a contamination profile and its specific source and/or food product should be of great interest to scientists working in the fields of contaminant analysis, toxicology and metabolism, as well as to regulatory bodies and

  16. Multiple Group Testing Procedures for Analysis of High-Dimensional Genomic Data

    Directory of Open Access Journals (Sweden)

    Hyoseok Ko

    2016-12-01

    Full Text Available In genetic association studies with high-dimensional genomic data, multiple group testing procedures are often required in order to identify disease/trait-related genes or genetic regions, where multiple genetic sites or variants are located within the same gene or genetic region. However, statistical testing procedures based on an individual test suffer from multiple testing issues such as the control of family-wise error rate and dependent tests. Moreover, detecting only a few of genes associated with a phenotype outcome among tens of thousands of genes is of main interest in genetic association studies. In this reason regularization procedures, where a phenotype outcome regresses on all genomic markers and then regression coefficients are estimated based on a penalized likelihood, have been considered as a good alternative approach to analysis of high-dimensional genomic data. But, selection performance of regularization procedures has been rarely compared with that of statistical group testing procedures. In this article, we performed extensive simulation studies where commonly used group testing procedures such as principal component analysis, Hotelling's T2 test, and permutation test are compared with group lasso (least absolute selection and shrinkage operator in terms of true positive selection. Also, we applied all methods considered in simulation studies to identify genes associated with ovarian cancer from over 20,000 genetic sites generated from Illumina Infinium HumanMethylation27K Beadchip. We found a big discrepancy of selected genes between multiple group testing procedures and group lasso.

  17. Obesogenic family types identified through latent profile analysis.

    Science.gov (United States)

    Martinson, Brian C; VazquezBenitez, Gabriela; Patnode, Carrie D; Hearst, Mary O; Sherwood, Nancy E; Parker, Emily D; Sirard, John; Pasch, Keryn E; Lytle, Leslie

    2011-10-01

    Obesity may cluster in families due to shared physical and social environments. This study aims to identify family typologies of obesity risk based on family environments. Using 2007-2008 data from 706 parent/youth dyads in Minnesota, we applied latent profile analysis and general linear models to evaluate associations between family typologies and body mass index (BMI) of youth and parents. Three typologies described most families with 18.8% "Unenriched/Obesogenic," 16.9% "Risky Consumer," and 64.3% "Healthy Consumer/Salutogenic." After adjustment for demographic and socioeconomic factors, parent BMI and youth BMI Z-scores were higher in unenriched/obesogenic families (BMI difference = 2.7, p < 0.01 and BMI Z-score difference = 0.51, p < 0.01, respectively) relative to the healthy consumer/salutogenic typology. In contrast, parent BMI and youth BMI Z-scores were similar in the risky consumer families relative to those in healthy consumer/salutogenic type. We can identify family types differing in obesity risks with implications for public health interventions.

  18. Obesogenic Family Types Identified through Latent Profile Analysis

    Science.gov (United States)

    VazquezBenitez, Gabriela; Patnode, Carrie D.; Hearst, Mary O.; Sherwood, Nancy E.; Parker, Emily D.; Sirard, John; Pasch, Keryn E.; Lytle, Leslie

    2011-01-01

    Background Obesity may cluster in families due to shared physical and social environments. Purpose This study aims to identify family typologies of obesity risk based on family environments. Methods Using 2007–2008 data from 706 parent/youth dyads in Minnesota, we applied latent profile analysis and general linear models to evaluate associations between family typologies and body mass index (BMI) of youth and parents. Results Three typologies described most families with 18.8% “Unenriched/Obesogenic,” 16.9% “Risky Consumer,” and 64.3% “Healthy Consumer/Salutogenic.” After adjustment for demographic and socioeconomic factors, parent BMI and youth BMI Z-scores were higher in unenriched/obesogenic families (BMI difference=2.7, p<0.01 and BMI Z-score difference=0.51, p<0.01, respectively) relative to the healthy consumer/salutogenic typology. In contrast, parent BMI and youth BMI Z-scores were similar in the risky consumer families relative to those in healthy consumer/salutogenic type. Conclusions We can identify family types differing in obesity risks with implications for public health interventions. PMID:21638195

  19. CSS Need Analysis: Theory and Computation Procedures for the 1979-80 FAF.

    Science.gov (United States)

    Case, Joe Paul; Cavanaugh, William J.

    This 1979-80 guide includes information about the basic rationale, the economic principles, and the computational procedures used in the College Scholarship Service need-analysis system. Contents include: an introduction to the principles and practices of student financial aid administration, the history of need analysis, the theory of need…

  20. Microarray gene expression profiling and analysis in renal cell carcinoma

    Directory of Open Access Journals (Sweden)

    Sadhukhan Provash

    2004-06-01

    Full Text Available Abstract Background Renal cell carcinoma (RCC is the most common cancer in adult kidney. The accuracy of current diagnosis and prognosis of the disease and the effectiveness of the treatment for the disease are limited by the poor understanding of the disease at the molecular level. To better understand the genetics and biology of RCC, we profiled the expression of 7,129 genes in both clear cell RCC tissue and cell lines using oligonucleotide arrays. Methods Total RNAs isolated from renal cell tumors, adjacent normal tissue and metastatic RCC cell lines were hybridized to affymatrix HuFL oligonucleotide arrays. Genes were categorized into different functional groups based on the description of the Gene Ontology Consortium and analyzed based on the gene expression levels. Gene expression profiles of the tissue and cell line samples were visualized and classified by singular value decomposition. Reverse transcription polymerase chain reaction was performed to confirm the expression alterations of selected genes in RCC. Results Selected genes were annotated based on biological processes and clustered into functional groups. The expression levels of genes in each group were also analyzed. Seventy-four commonly differentially expressed genes with more than five-fold changes in RCC tissues were identified. The expression alterations of selected genes from these seventy-four genes were further verified using reverse transcription polymerase chain reaction (RT-PCR. Detailed comparison of gene expression patterns in RCC tissue and RCC cell lines shows significant differences between the two types of samples, but many important expression patterns were preserved. Conclusions This is one of the initial studies that examine the functional ontology of a large number of genes in RCC. Extensive annotation, clustering and analysis of a large number of genes based on the gene functional ontology revealed many interesting gene expression patterns in RCC. Most

  1. Geocoronal Balmer α line profile observations and forward-model analysis

    Science.gov (United States)

    Mierkiewicz, E. J.; Bishop, J.; Roesler, F. L.; Nossal, S. M.

    2006-05-01

    High spectral resolution geocoronal Balmer α line profile observations from Pine Bluff Observatory (PBO) are presented in the context of forward-model analysis. Because Balmer series column emissions depend significantly on multiple scattering, retrieval of hydrogen parameters of general aeronomic interest from these observations (e.g., the hydrogen column abundance) currently requires a forward modeling approach. This capability is provided by the resonance radiative transfer code LYAO_RT. We have recently developed a parametric data-model comparison search procedure employing an extensive grid of radiative transport model input parameters (defining a 6-dimensional parameter space) to map-out bounds for feasible forward model retrieved atomic hydrogen density distributions. We applied this technique to same-night (March, 2000) ground-based Balmer α data from PBO and geocoronal Lyman β measurements from the Espectrógrafo Ultravioleta extremo para la Radiación Difusa (EURD) instrument on the Spanish satellite MINISAT-1 (provided by J.F. Gómez and C. Morales of the Laboratorio de Astrofisica Espacial y Física Fundamental, INTA, Madrid, Spain) in order to investigate the modeling constraints imposed by two sets of independent geocoronal intensity measurements, both of which rely on astronomical calibration methods. In this poster we explore extending this analysis to the line profile information also contained in the March 2000 PBO Balmer α data set. In general, a decrease in the Doppler width of the Balmer α emission with shadow altitude is a persistent feature in every night of PBO observations in which a wide range of shadow altitudes are observed. Preliminary applications of the LYAO_RT code, which includes the ability to output Doppler line profiles for both the singly and multiply scattered contributions to the Balmer α emission line, displays good qualitative agreement with regard to geocoronal Doppler width trends observed from PBO. Model-data Balmer

  2. Effect of Music on Outpatient Urological Procedures: A Systematic Review and Meta-Analysis from ESUT.

    Science.gov (United States)

    Kyriakides, Rena; Jones, Patrick; Geraghty, Robert; Skolarikos, Andreas; Liatsikos, Evangellos; Traxer, Olivier; Pietropaolo, Amelia; Somani, Bhaskar K

    2017-12-07

    Music is a practical, inexpensive and harmless analgesic and anxiolytic. An increasing number of original studies have been performed to investigate its potential application in urology. Our aim was to identify the effect of music on outpatient based urological procedures. We systematically reviewed the effect of using music during all reported outpatient urology procedures, including transrectal ultrasound guided prostate biopsy, shock wave lithotripsy, urodynamic studies, percutaneous nephrostomy tube placement and cystoscopy. Data were included on all randomized trials from 1980 to 2017 and no language restrictions were applied. Included in analysis were 16 randomized studies in which 972 of 1,950 patients (49.8%) were exposed to music during an outpatient procedure. The procedures included transrectal ultrasound guided prostate biopsy in 4 studies in a total of 286 patients, shock wave lithotripsy in 6 studies in a total of 1023, cystoscopy in 3 studies in a total of 331, urodynamics in 2 studies in a total of 210 and percutaneous nephrostomy in 1 study in a total of 100. All studies incorporated a visual analog score to measure pain. Anxiety was measured by STAI (State-Trait anxiety Inventory) in 13 studies and by a visual analog scale in 2. While 14 of the 16 studies showed a reduction in self-reported pain, a reduction in anxiety was seen in 14. When using music, overall procedural satisfaction was better in 9 studies and patient willingness to repeat the procedure was also higher in 7. Our meta-analysis revealed a significant reduction in visual analog scale and STAI findings across all studies (p <0.001). Our systematic review demonstrated a beneficial effect of music on urological outpatient procedures. Music seemed to decrease anxiety and pain. It might serve as a useful adjunct to increase procedural satisfaction and patient willingness to undergo the procedure again. Copyright © 2018 American Urological Association Education and Research, Inc

  3. Analysis of the phospholipid profile of metaphase II mouse oocytes undergoing vitrification.

    Directory of Open Access Journals (Sweden)

    Jaehun Jung

    Full Text Available Oocyte freezing confers thermal and chemical stress upon the oolemma and various other intracellular structures due to the formation of ice crystals. The lipid profiles of oocytes and embryos are closely associated with both, the degrees of their membrane fluidity, as well as the degree of chilling and freezing injuries that may occur during cryopreservation. In spite of the importance of lipids in the process of cryopreservation, the phospholipid status in oocytes and embryos before and after freezing has not been investigated. In this study, we employed mass spectrometric analysis to examine if vitrification has an effect on the phospholipid profiles of mouse oocytes. Freshly prepared metaphase II mouse oocytes were vitrified using copper grids and stored in liquid nitrogen for 2 weeks. Fresh and vitrified-warmed oocytes were subjected to phospholipid extraction procedure. Mass spectrometric analyses revealed that multiple species of phospholipids are reduced in vitrified-warmed oocytes. LIFT analyses identified 31 underexpressed and 5 overexpressed phospholipids in vitrified mouse oocytes. The intensities of phosphatidylinositol (PI {18∶2/16∶0} [M-H]- and phosphatidylglycerol (PG {14∶0/18∶2} [M-H]- were decreased the most with fold changes of 30.5 and 19.1 in negative ion mode, respectively. Several sphingomyelins (SM including SM {d38∶3} [M+H]+ and SM {d34∶0} [M+K]+ were decreased significantly in positive ion mode. Overall, the declining trend of multiple phospholipids demonstrates that vitrification has a marked effect on phospholipid profiles of oocytes. These results show that the identified phospholipids can be used as potential biomarkers of oocyte undergoing vitrification and will allow for the development of strategies to preserve phospholipids during oocyte cryopreservation.

  4. Flash Study Analysis and the Music Learning Pro-Files Project

    Science.gov (United States)

    Cremata, Radio; Pignato, Joseph; Powell, Bryan; Smith, Gareth Dylan

    2016-01-01

    This paper introduces the Music Learning Profiles Project, and its methodological approach, flash study analysis. Flash study analysis is a method that draws heavily on extant qualitative approaches to education research, to develop broad understandings of music learning in diverse contexts. The Music Learning Profiles Project (MLPP) is an…

  5. Examining Combinations of Social Physique Anxiety and Motivation Regulations Using Latent Profile Analysis

    Science.gov (United States)

    Ullrich-French, Sarah; Cox, Anne E.; Cooper, Brittany Rhoades

    2016-01-01

    Previous research has used cluster analysis to examine how social physique anxiety (SPA) combines with motivation in physical education. This study utilized a more advanced analytic approach, latent profile analysis (LPA), to identify profiles of SPA and motivation regulations. Students in grades 9-12 (N = 298) completed questionnaires at two time…

  6. Sex determination of human skeletal populations using latent profile analysis.

    Science.gov (United States)

    Passalacqua, Nicholas V; Zhang, Zhen; Pierce, Steven J

    2013-08-01

    Accurately estimating biological sex from the human skeleton can be especially difficult for fragmentary or incomplete remains often encountered in bioarchaeological contexts. Where typical anatomically dimorphic skeletal regions are incomplete or absent, observers often take their best guess to classify biological sex. Latent profile analysis (LPA) is a mixture modeling technique which uses observed continuous data to estimate unobserved categorical group membership using posterior probabilities. In this study, sex is the latent variable (male and female are the two latent classes), and the indicator variables used here were eight standard linear measurements (long bone lengths, diaphyseal and articular breadths, and circumferences). Mplus (Muthén and Muthén: Mplus user's guide, 6th ed. Los Angeles: Muthén & Muthén, 2010) was used to obtain maximum likelihood estimates for latent class membership from a known sample of individuals from the forensic data bank (FDB) (Jantz and Moore-Jansen: Database for forensic anthropology in the United States 1962-1991, Ann Arbor, MI: Interuniversity Consortium for Political and Social Research, 2000) (n = 1,831), yielding 87% of correct classification for sex. Then, a simulation extracted 5,000 different random samples of 206 complete cases each from the FDB (these cases also had known sex). We then artificially imposed patterns of missing data similar to that observed in a poorly preserved bioarchaeological sample from Medieval Asturias, Spain (n = 206), and ran LPA on each sample. This tested the efficacy of LPA under extreme conditions of poor preservation (missing data, 42%). The simulation yielded an average of 82% accuracy, indicating that LPA is robust to large amounts of missing data when analyzing incomplete skeletons. Copyright © 2013 Wiley Periodicals, Inc.

  7. Analysis and design of low profile multiband multifunctional antenna arrays

    Science.gov (United States)

    Hunsicker, Walker F.

    Light-weight phased array antennas for aerospace and mobile applications require utilizing the same antenna aperture to provide multiple functions with dissimilar radiation pattern specifications (e.g., multiband operation for communications and tracking). Multi-functional antennas provide advantages over aggregate antenna clusters by reducing space requirements, and can aid in the optimal placement of all required apertures to provide adequate isolation between channels. Furthermore, the combination of antenna apertures into a common geometry mitigates co-site installation issues by addressing interference within the integrated radiator design itself as opposed to the extensive analysis which is required to configure multiple radiators in close proximity. The combination of multiple radiators into a single aperture can only be achieved with the proper selection of antenna topology and accompanying feed network design. This research proposes a new technique for the design of multiband arrays in which a common aperture is used. Highlighted by this method is the integration of a tri-band array comprised of an X-band (12 GHz) microstrip patch array on a superstrate above printed dual-band (1 and 2 GHz) slot loop antenna arrays in an octave-spaced lattice. The selection of a ground backing reflector is considered for improved gain and system packaging, but restricts the utility of the design principally due to the lambda/4 depth of the ground plane. Therefore, a novel multiband high impedance surfaces (HIS) is proposed to load the slot apertures for reduced height. The novel techniques proposed here will enable the design of a low profile and conformal single aperture supporting multi-band and multi-functional operations.

  8. Tissue compartment analysis for biomarker discovery by gene expression profiling.

    Directory of Open Access Journals (Sweden)

    Antoine Disset

    Full Text Available BACKGROUND: Although high throughput technologies for gene profiling are reliable tools, sample/tissue heterogeneity limits their outcomes when applied to identify molecular markers. Indeed, inter-sample differences in cell composition contribute to scatter the data, preventing detection of small but relevant changes in gene expression level. To date, attempts to circumvent this difficulty were based on isolation of the different cell structures constituting biological samples. As an alternate approach, we developed a tissue compartment analysis (TCA method to assess the cell composition of tissue samples, and applied it to standardize data and to identify biomarkers. METHODOLOGY/PRINCIPAL FINDINGS: TCA is based on the comparison of mRNA expression levels of specific markers of the different constitutive structures in pure isolated structures, on the one hand, and in the whole sample on the other. TCA method was here developed with human kidney samples, as an example of highly heterogeneous organ. It was validated by comparison of the data with those obtained by histo-morphometry. TCA demonstrated the extreme variety of composition of kidney samples, with abundance of specific structures varying from 5 to 95% of the whole sample. TCA permitted to accurately standardize gene expression level amongst >100 kidney biopsies, and to identify otherwise imperceptible molecular disease markers. CONCLUSIONS/SIGNIFICANCE: Because TCA does not require specific preparation of sample, it can be applied to all existing tissue or cDNA libraries or to published data sets, inasmuch specific operational compartments markers are available. In human, where the small size of tissue samples collected in clinical practice accounts for high structural diversity, TCA is well suited for the identification of molecular markers of diseases, and the follow up of identified markers in single patients for diagnosis/prognosis and evaluation of therapy efficiency. In laboratory

  9. The analysis of typical profile clearances formation in meshing rotors of the screw compressor

    Science.gov (United States)

    Mustafin, T. N.; Yakupov, R. R.; Khamidullin, M. S.; Khisameev, I. G.; Alyaev, V. A.; Paranina, O. Yu.

    2017-08-01

    The improvement of screw compressor energetic characteristics is related to their working process enhancement. In many ways, it depends on the gas leakage between working chambers through the gaps. One of the main gaps in the screw compressor is the rotor profile clearances. They also generally determine the smoothness of meshing, partially influencing vibration levels. The rotor profile clearances occur due to the reduction of the real rotor profile in relation to the theoretical profile. It is done to compensate negative factors such as a rotor thermal deformation, manufacturing errors, etc. Their deep analysis may help to improve the real rotor profiling method and increase the screw compressor energetic characteristics.

  10. Reliability assessment of a manual-based procedure towards learning curve modeling and fmea analysis

    Directory of Open Access Journals (Sweden)

    Gustavo Rech

    2013-03-01

    Full Text Available Separation procedures in drug Distribution Centers (DC are manual-based activities prone to failures such as shipping exchanged, expired or broken drugs to the customer. Two interventions seem as promising in improving the reliability in the separation procedure: (i selection and allocation of appropriate operators to the procedure, and (ii analysis of potential failure modes incurred by selected operators. This article integrates Learning Curves (LC and FMEA (Failure Mode and Effect Analysis aimed at reducing the occurrence of failures in the manual separation of a drug DC. LCs parameters enable generating an index to identify the recommended operators to perform the procedures. The FMEA is then applied to the separation procedure carried out by the selected operators in order to identify failure modes. It also deployed the traditional FMEA severity index into two sub-indexes related to financial issues and damage to company´s image in order to characterize failures severity. When applied to a drug DC, the proposed method significantly reduced the frequency and severity of failures in the separation procedure.

  11. Power analysis for multivariate and repeated measures designs: a flexible approach using the SPSS MANOVA procedure.

    Science.gov (United States)

    D'Amico, E J; Neilands, T B; Zambarano, R

    2001-11-01

    Although power analysis is an important component in the planning and implementation of research designs, it is often ignored. Computer programs for performing power analysis are available, but most have limitations, particularly for complex multivariate designs. An SPSS procedure is presented that can be used for calculating power for univariate, multivariate, and repeated measures models with and without time-varying and time-constant covariates. Three examples provide a framework for calculating power via this method: an ANCOVA, a MANOVA, and a repeated measures ANOVA with two or more groups. The benefits and limitations of this procedure are discussed.

  12. A retrospective study of root coverage procedures using an image analysis system.

    Science.gov (United States)

    Kerner, Stéphane; Borghetti, Alain; Katsahian, Sandrine; Etienne, Daniel; Malet, Jacques; Mora, Francis; Monnet-Corti, Virginie; Glise, Jean-Marc; Bouchard, Philippe

    2008-04-01

    To investigate the efficacy of root coverage procedures and factors that may affect the clinical outcomes in non-experimental patients. Two hundred and eighty-seven root coverage surgical procedures in 215 adult patients were evaluated retrospectively. Descriptive statistics were used to determine the patient profile. Comparisons between surgeries were assessed, and the impact of different parameters on the probability of mean/complete root coverage and gingival augmentation was explored. The mean percentage of root coverage was 72.29 (+/- 28)%. Complete root coverage was observed in 35.56% of the defects. The difference between the surgical procedures was not significant. The mean percentage of gingival augmentation was 106.18 (+/- 260)%. The difference between non-submerged grafts and the other techniques was significant (pimpact of smoking, and maxillary teeth for both mean and complete root coverage were found. A significant positive impact of the tuberosity donor site was found for complete root coverage. Maxillary teeth and Miller's Class II and III were positive predictive factors for gingival augmentation. Under non-experimental conditions, root coverage procedures are effective. Smoking, maxillary teeth, donor site, and Miller's Classes are prognostic factors that may affect the results.

  13. Analysis of lipid profile in cancer patients, smokers, and nonsmokers.

    Science.gov (United States)

    Reddy, A Vikramsimha; Killampalli, Lakshmi Keerthana; Prakash, A Ravi; Naag, Sushma; Sreenath, G; Biraggari, Sunil Kumar

    2016-01-01

    Lipids play an important role in maintaining the cell membrane integrity. Lipid profile is a panel of blood tests that serve as an initial medical screening for abnormalities in lipids and approximate risk for cancer, cardiovascular diseases, pancreatitis, etc., The present study evaluates the alterations in lipid profile in cancer patients, smokers, and nonsmokers and aims to achieve a correlation between them. The study is an in vitro type of cross-sectional study with 25 oral cancer patients, 25 chronic smokers (habit persisting for 15 years or more), and 15 nonsmokers as control group. Blood samples had been collected, and triglycerides (TGs), total cholesterol (TC), high-density lipoprotein (HDL), very low-density lipoprotein (VLDL), and low-density lipoprotein (LDL) were analyzed using a lipid profile kit and an autoanalyzer. The results were analyzed using the unpaired t-test and ANOVA test (P lipid profile in smokers and cancer patients. The decrease in lipid profile in cancer patients might be due to their increased utilization of lipids by neoplastic cells in membrane biogenesis. Therefore, a decrease in lipid profile in smokers can be assumed that they might be more prone to develop cancerous conditions.

  14. A Review on Innovative Procedures for the Analysis of Data from Gas Sensor Systems and Networks

    Directory of Open Access Journals (Sweden)

    Rolf Seifert

    2015-01-01

    Full Text Available Economic online and in-situ field analyses applications like discriminated alarming of smoldering fire or toxic gas leakages, monitoring of volatile components in chemical and biochemical processes, quality monitoring in food processing etc., wait for reliable and economic analytical solutions by sensor systems. This paper presents various innovative mathematical procedures to analyze data from gas sensor systems and gas sensor nets: ProSens - an efficient mathematical procedure for calibration and evaluation of tin oxide gas sensor data, SimSens – a mathematical program for simultaneous analysis of gases, ProCal - a program for batch-wise calibration of multi gas sensors and ProSource - a procedure for source localization. Applications to real data demonstrate the performance of the procedures.

  15. Comparison of Dissolution Similarity Assessment Methods for Products with Large Variations: f2 Statistics and Model-Independent Multivariate Confidence Region Procedure for Dissolution Profiles of Multiple Oral Products.

    Science.gov (United States)

    Yoshida, Hiroyuki; Shibata, Hiroko; Izutsu, Ken-Ichi; Goda, Yukihiro

    2017-01-01

    The current Japanese Ministry of Health Labour and Welfare (MHLW)'s Guideline for Bioequivalence Studies of Generic Products uses averaged dissolution rates for the assessment of dissolution similarity between test and reference formulations. This study clarifies how the application of model-independent multivariate confidence region procedure (Method B), described in the European Medical Agency and U.S. Food and Drug Administration guidelines, affects similarity outcomes obtained empirically from dissolution profiles with large variations in individual dissolution rates. Sixty-one datasets of dissolution profiles for immediate release, oral generic, and corresponding innovator products that showed large variation in individual dissolution rates in generic products were assessed on their similarity by using the f2 statistics defined in the MHLW guidelines (MHLW f2 method) and two different Method B procedures, including a bootstrap method applied with f2 statistics (BS method) and a multivariate analysis method using the Mahalanobis distance (MV method). The MHLW f2 and BS methods provided similar dissolution similarities between reference and generic products. Although a small difference in the similarity assessment may be due to the decrease in the lower confidence interval for expected f2 values derived from the large variation in individual dissolution rates, the MV method provided results different from those obtained through MHLW f2 and BS methods. Analysis of actual dissolution data for products with large individual variations would provide valuable information towards an enhanced understanding of these methods and their possible incorporation in the MHLW guidelines.

  16. Development of the HELIOS/MASTER 2-Step Procedure for the Prismatic VHTR Physics Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kang Seog; Cho, Jin Young; Noh, Jae Man

    2007-05-15

    A new physics analysis procedure has been developed for a prismatic very high temperature gas-cooled reactor based on a conventional two-step procedure for the PWR physics analysis. The HELIOS and MASTER codes were employed to generate the coarse group cross sections through a transport lattice calculation, and to perform the 3-dimensional core physics analysis by a nodal diffusion calculation, respectively. Physics analysis of the prismatic VHTRs involves particular modeling issues such as a double heterogeneity of the coated fuel particles, a neutron streaming in the coolant channels, a strong core-reflector interaction, and large spectrum shifts due to changes of the surrounding environment and state parameters. Double heterogeneity effect was considered by using a recently developed reactivity equivalent physical transformation method. Neutron streaming effect was quantified through 3-dimensional Monte Carlo transport calculations by using the MCNP code. Strong core-reflector interaction could be handled by applying an equivalence theory to the generation of the reflector cross sections. The effects of a spectrum shift could be covered by optimizing the coarse energy group structure. A two-step analysis procedure was established for the prismatic VHTR physics analysis by combining all the methodologies described above. The applicability of our code system was tested against core benchmark problems. The results of these benchmark tests show that our code system is very accurate and practical for a prismatic VHTR physics analysis.

  17. Photometric analysis of esthetically pleasant and unpleasant facial profile

    Directory of Open Access Journals (Sweden)

    Helena Nunes da Rocha Fortes

    2014-04-01

    Full Text Available Objective: To identify which linear, angular and proportionality measures could influence a profile to be considered esthetically pleasant or unpleasant, and to assess sexual dimorphism. Methods: 150 standardized facial profile photographs of dental students of both sexes were obtained and printed on photographic paper. Ten plastic surgeons, ten orthodontists and ten layperson answered a questionnaire characterizing each profile as pleasant, acceptable or unpleasant. With the use of a score system, the 15 most pleasant and unpleasant profiles of each sex were selected. The photographs were scanned into AutoCAD computer software. Linear, angular and proportion measurements were obtained using the software tools. The average values between groups were compared by the Student's t-test and the Mann-Whitney test at 5%. Results: The linear measures LL-S, LL-H, LL-E, LL-B and Pn-H showed statistically significant differences (p < 0.05. Statistical differences were also found in the angular measures G'.Pn.Pg', G'.Sn.Pg' and Sn.Me'.C and in the proportions G'-Sn:Sn-Me' and Sn-Gn':Gn'-C (p < 0.05. Differences between sexes were found for the linear measure Ala-Pn, angles G'-Pg'.N-Pn, Sn.Me'.C, and proportions Gn'-Sn:Sn-Me' and Ala-Pn:N'-Sn. (p < 0.05. Conclusion: The anteroposterior position of the lower lip, the amount of nose that influences the profile, facial convexity, total vertical proportion and lip-chin proportion appear to influence pleasantness of facial profile. Sexual dimorphism was identified in nasal length, nasofacial and lower third of the face angles, total vertical and nasal height/length proportions.

  18. Procedural Principles of Design : The Role of Analysis and Evaluation in Educational Design Processes

    NARCIS (Netherlands)

    Visscher-Voerman, Irene

    2007-01-01

    Educational designers conduct a variety of design activities in practice. Building on several reconstructive studies of design practice, 8 procedural design principles, related to analysis and formative evaluation activities were formulated and discussed and elaborated in a one-day workshop with 24

  19. An Analysis of Meehl's MAXCOV-HITMAX Procedure for the Case of Dichotomous Indicators.

    Science.gov (United States)

    Maraun, Michael D.; Slaney, Kathleen; Goddyn, Louis

    2003-01-01

    Analyzed the performance of the MAXCOV-HITMAX procedure invented by Paul Meehl for the detection of latent taxonic structures in which the latent variable, theta, is Bernoulli distributed for the case of dichotomous indicators. Discusses the implications of the analysis for the case of MAXCOV-HIMAX with continuous indicators. (SLD)

  20. Procedural and Conceptual Difficulties with Slope: An Analysis of Students' Mistakes on Routine Tasks

    Science.gov (United States)

    Cho, Peter; Nagle, Courtney

    2017-01-01

    This study extends past research on students' understanding of slope by analyzing college students' mistakes on routine tasks involving slope. We conduct both quantitative and qualitative analysis of students' mistakes on common slope tasks to extract information regarding procedural proficiencies and conceptual underpinnings required in order for…

  1. Confirmatory factor analysis of the Child Oral Health Impact Profile (Korean version).

    Science.gov (United States)

    Cho, Young Il; Lee, Soonmook; Patton, Lauren L; Kim, Hae-Young

    2016-04-01

    Empirical support for the factor structure of the Child Oral Health Impact Profile (COHIP) has not been fully established. The purposes of this study were to evaluate the factor structure of the Korean version of the COHIP (COHIP-K) empirically using confirmatory factor analysis (CFA) based on the theoretical framework and then to assess whether any of the factors in the structure could be grouped into a simpler single second-order factor. Data were collected through self-reported COHIP-K responses from a representative community sample of 2,236 Korean children, 8-15 yr of age. Because a large inter-factor correlation of 0.92 was estimated in the original five-factor structure, the two strongly correlated factors were combined into one factor, resulting in a four-factor structure. The revised four-factor model showed a reasonable fit with appropriate inter-factor correlations. Additionally, the second-order model with four sub-factors was reasonable with sufficient fit and showed equal fit to the revised four-factor model. A cross-validation procedure confirmed the appropriateness of the findings. Our analysis empirically supported a four-factor structure of COHIP-K, a summarized second-order model, and the use of an integrated summary COHIP score. © 2016 Eur J Oral Sci.

  2. Community Violence, Protective Factors, and Adolescent Mental Health: A Profile Analysis

    OpenAIRE

    Copeland-Linder, Nikeea; LAMBERT, SHARON F.; Ialongo, Nicholas S.

    2010-01-01

    This study examined interrelationships among community violence exposure, protective factors, and mental health in a sample of urban, predominantly African American adolescents (N = 504). Latent Profile Analysis was conducted to identify profiles of adolescents based on a combination of community violence exposure, self-worth, parental monitoring, and parental involvement and to examine whether these profiles differentially predict adolescents' depressive symptoms and aggressive behavior. Thr...

  3. Procedural instruction in invasive bedside procedures: a systematic review and meta-analysis of effective teaching approaches.

    Science.gov (United States)

    Huang, Grace C; McSparron, Jakob I; Balk, Ethan M; Richards, Jeremy B; Smith, C Christopher; Whelan, Julia S; Newman, Lori R; Smetana, Gerald W

    2016-04-01

    Optimal approaches to teaching bedside procedures are unknown. To identify effective instructional approaches in procedural training. We searched PubMed, EMBASE, Web of Science and Cochrane Library through December 2014. We included research articles that addressed procedural training among physicians or physician trainees for 12 bedside procedures. Two independent reviewers screened 9312 citations and identified 344 articles for full-text review. Two independent reviewers extracted data from full-text articles. We included measurements as classified by translational science outcomes T1 (testing settings), T2 (patient care practices) and T3 (patient/public health outcomes). Due to incomplete reporting, we post hoc classified study outcomes as 'negative' or 'positive' based on statistical significance. We performed meta-analyses of outcomes on the subset of studies sharing similar outcomes. We found 161 eligible studies (44 randomised controlled trials (RCTs), 34 non-RCTs and 83 uncontrolled trials). Simulation was the most frequently published educational mode (78%). Our post hoc classification showed that studies involving simulation, competency-based approaches and RCTs had higher frequencies of T2/T3 outcomes. Meta-analyses showed that simulation (risk ratio (RR) 1.54 vs 0.55 for studies with vs without simulation, p=0.013) and competency-based approaches (RR 3.17 vs 0.89, peffective forms of training. This systematic review of bedside procedural skills demonstrates that the current literature is heterogeneous and of varying quality and rigour. Evidence is strongest for the use of simulation and competency-based paradigms in teaching procedures, and these approaches should be the mainstay of programmes that train physicians to perform procedures. Further research should clarify differences among instructional methods (eg, forms of hands-on training) rather than among educational modes (eg, lecture vs simulation). Published by the BMJ Publishing Group Limited

  4. Analysis of paediatric prescribing profiles in two health-funding ...

    African Journals Online (AJOL)

    Setting. Two industry databases, one covering a prepaid health maintenance organisation (HMO), the other providing a chronic medication programme for medical schemes and their members. Main outcome measures. Comparison of prescribing profiles of specialists and GPs. Main results. Data from the HMO revealed that ...

  5. Magnetic field profile analysis for gyrotron experimental investigation

    Science.gov (United States)

    Pagonakis, Ioannis Gr.; Avramidis, Konstantinos A.; Gantenbein, Gerd; Rzesnicki, Tomasz; Samartsev, Andrey; Jelonnek, John

    2017-03-01

    The external applied magnetic field plays a main role on the gyrotron operation. Even if the gyrotron design is optimized for the nominal magnetic profile, it is possible the performance to be better for an alternative one. This unexpected result can happen for several reasons, such as the manufacturing imperfections, the misalignment issues, and several unknown factors in gyrotron theory and design. The systematic experimental investigation of the gyrotron in different magnetic profiles is very important for the optimization of the gyrotron operation and for the better understanding of the gyrotron behavior. In this sense, an analytical approach for the definition of the appropriate magnetic profiles based on the beam characteristics instead of the coil currents definition is proposed for a systematic experimental study. Using this technique, operational maps in the space of the important magnetic profile parameters are developed, which are very useful for the characterization of the gyrotron performance. For the demonstration of this idea, the operational maps of the short-pulse prototype of the 170 GHz, 1 MW European ITER gyrotron project are presented.

  6. PROFFIT: Analysis of X-ray surface-brightness profiles

    Science.gov (United States)

    Eckert, Dominique

    2016-08-01

    PROFFIT analyzes X-ray surface-brightness profiles for data from any X-ray instrument. It can extract surface-brightness profiles in circular or elliptical annuli, using constant or logarithmic bin size, from the image centroid, the surface-brightness peak, or any user-given center, and provides surface-brightness profiles in any circular or elliptical sectors. It offers background map support to extract background profiles, can excise areas using SAO DS9-compatible (ascl:0003.002) region files to exclude point sources, provides fitting with a number of built-in models, including the popular beta model, double beta, cusp beta, power law, and projected broken power law, uses chi-squared or C statistic, and can fit on the surface-brightness or counts data. It has a command-line interface similar to HEASOFT’s XSPEC (ascl:9910.005) package, provides interactive help with a description of all the commands, and results can be saved in FITS, ROOT or TXT format.

  7. a comparative analysis of the sociolinguistic profiles of english in ...

    African Journals Online (AJOL)

    This paper compares the historical and sociolinguistic contexts of English in Kenya and Uganda addressing several questions such as: What are the similarities and differences in the sociolinguistic profiles of English in these two East African countries? How do the roles English plays in Kenya compare to its roles in ...

  8. A comparative analysis of the sociolinguistic profiles of English in ...

    African Journals Online (AJOL)

    Abstract. This paper compares the historical and sociolinguistic contexts of English in Kenya and Uganda addressing several questions such as: What are the similarities and differences in the sociolinguistic profiles of English in these two East African countries? How do the roles English plays in Kenya compare to its roles ...

  9. A comparative analysis of the sociolinguistic profiles of English in ...

    African Journals Online (AJOL)

    This paper compares the historical and sociolinguistic contexts of English in Kenya and Uganda addressing several questions such as: What are the similarities and differences in the sociolinguistic profiles of English in these two East African countries? How do the roles English plays in Kenya compare to its roles in ...

  10. Physical activity and body composition, a risk profile analysis of ...

    African Journals Online (AJOL)

    The globally enhanced prevalence of obesity and physical inactivity of children is a cause for major concern to health professionals. Very little is known about this phenomenon in Namibia. Therefore the aim of this study was to construct a profile of secondary school learners in selected urban schools in Namibia.

  11. Analysis of Bridge Player Profiles According to Their Intelligence Areas

    Science.gov (United States)

    Bilir, Fatma Pervin; Sirin, Yeliz

    2017-01-01

    The aim of this study is to figure out profiles of bridge players and analyzing them according to their intelligence areas. The sample of the study is consist of 100 volunteers out of 200 bridge players who have attended "Çukurova open double bridge championship" in Adana, Turkey at February 2016. Data have been collected via…

  12. Risk Profiles of Children Entering Residential Care: A Cluster Analysis

    Science.gov (United States)

    Hagaman, Jessica L.; Trout, Alexandra L.; Chmelka, M. Beth; Thompson, Ronald W.; Reid, Robert

    2010-01-01

    Children in residential care are a heterogeneous population, presenting various combinations of risks. Existing studies on these children suggest high variability across multiple domains (e.g., academics, behavior). Given this heterogeneity, it is important to begin to identify the combinations and patterns of multiple risks, or risk profiles,…

  13. Travel and Tourism Marketing. Ohio's Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    Developed through a modified DACUM (Developing a Curriculum) process involving business, industry, labor, and community agency representatives in Ohio, this document is a comprehensive and verified employer competency profile for travel and tourism occupations. The list contains units (with and without subunits), competencies, and competency…

  14. Multicriteria decision analysis in ranking of analytical procedures for aldrin determination in water.

    Science.gov (United States)

    Tobiszewski, Marek; Orłowski, Aleksander

    2015-03-27

    The study presents the possibility of multi-criteria decision analysis (MCDA) application when choosing analytical procedures with low environmental impact. A type of MCDA, Preference Ranking Organization Method for Enrichment Evaluations (PROMETHEE), was chosen as versatile tool that meets all the analytical chemists--decision makers requirements. Twenty five analytical procedures for aldrin determination in water samples (as an example) were selected as input alternatives to MCDA analysis. Nine different criteria describing the alternatives were chosen from different groups--metrological, economical and the most importantly--environmental impact. The weights for each criterion were obtained from questionnaires that were sent to experts, giving three different scenarios for MCDA results. The results of analysis show that PROMETHEE is very promising tool to choose the analytical procedure with respect to its greenness. The rankings for all three scenarios placed solid phase microextraction and liquid phase microextraction--based procedures high, while liquid-liquid extraction, solid phase extraction and stir bar sorptive extraction--based procedures were placed low in the ranking. The results show that although some of the experts do not intentionally choose green analytical chemistry procedures, their MCDA choice is in accordance with green chemistry principles. The PROMETHEE ranking results were compared with more widely accepted green analytical chemistry tools--NEMI and Eco-Scale. As PROMETHEE involved more different factors than NEMI, the assessment results were only weakly correlated. Oppositely, the results of Eco-Scale assessment were well-correlated as both methodologies involved similar criteria of assessment. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. The Sensitivity Analysis of Axial Pressure Tube Creep Profile for Dryout Power in PHWR

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, Euiseung; Kim, Youngae [KHNP CRI, Daejeon (Korea, Republic of)

    2016-10-15

    The Stern Laboratory performed the CHF tests with only one axial pressure tube creep profile per 3.3%, 5.1% peak crept channel and made CHF correlation including creep factor from the CHF test results. Wolsong nuclear power plants also have utilized the same CHF correlation derived by CNL. Pressure tube diameter creep rate is function of fast neutron, coolant temperature, and coolant pressure in a channel. It means that various axial pressure tube creep profiles exist in PHWR due to the history of operating conditions. Usually, CHF correlation is used during ROP(Regional Overpower Protection) Trip Setpoint Analysis or Safety Analysis in PHWR. The sensitivity analysis for CHF effects using various creep profiles is needed. This paper summarizes the comparison results of dryout power between CHF test creep profile and estimated creep profiles of Wolsong units. The effect of axial pressure tube creep profile for dryout power in fuel channel is evaluated by using Stern Lab. CHF test creep profile and 380 channel creep profiles of Wolsong. The dryout powers at 3.3% and 5.1% test conditions are slightly smaller when using 380 Wolsong channels creep profiles. These also show that the simulated dryout powers maintain consistency regardless of flow conditions.

  16. Analysis of procedures used in tooth avulsion by 100 dental surgeons.

    Science.gov (United States)

    Manfrin, Thais Mara; Boaventura, Renata Santos; Poi, Wilson Roberto; Panzarini, Sônia Regina; Sonoda, Celso Koogi; Massa Sundefeld, Maria Lúcia Marçal

    2007-08-01

    Accurate diagnosis and adequate treatment plan may constitute very complex tasks, particularly in tooth avulsion, because several variables are involved. In addition to the technical knowledge and clinical experience directed toward the quality of treatment, patient education may favorably influence the survival of replanted teeth. The aim of this study was to analyze the procedures used in the management of tooth avulsion by 100 dental surgeons (DSs). Thus, by means of a descriptive questionnaire, information was obtained about the profile of the professionals interviewed, procedures used in cases of tooth avulsion, and patient orientation and education. One hundred properly filled questionnaires were obtained. Descriptive statistics was used for the data, and the chi-squared statistics was employed (epi-info 3.2 software). According to the results, this type of trauma is part of the routine of 15 DSs, although 71 have reported some experience with avulsed teeth. Great deficiencies were found regarding root surface treatment and occlusal adjustment. Positive findings were related to socket treatment, adjunctive therapy, and patient education and orientation (extra-alveolar period, storage medium, manipulation of the avulsed tooth, replantation by the own patient). It was possible to conclude that 47.5% of the procedures reported by 100 professionals interviewed are adequate, and patient education is favorable in 87.7% of cases, a fact that can positively interfere with the prognosis of tooth replantation.

  17. A P-value model for theoretical power analysis and its applications in multiple testing procedures

    Directory of Open Access Journals (Sweden)

    Fengqing Zhang

    2016-10-01

    Full Text Available Abstract Background Power analysis is a critical aspect of the design of experiments to detect an effect of a given size. When multiple hypotheses are tested simultaneously, multiplicity adjustments to p-values should be taken into account in power analysis. There are a limited number of studies on power analysis in multiple testing procedures. For some methods, the theoretical analysis is difficult and extensive numerical simulations are often needed, while other methods oversimplify the information under the alternative hypothesis. To this end, this paper aims to develop a new statistical model for power analysis in multiple testing procedures. Methods We propose a step-function-based p-value model under the alternative hypothesis, which is simple enough to perform power analysis without simulations, but not too simple to lose the information from the alternative hypothesis. The first step is to transform distributions of different test statistics (e.g., t, chi-square or F to distributions of corresponding p-values. We then use a step function to approximate each of the p-value’s distributions by matching the mean and variance. Lastly, the step-function-based p-value model can be used for theoretical power analysis. Results The proposed model is applied to problems in multiple testing procedures. We first show how the most powerful critical constants can be chosen using the step-function-based p-value model. Our model is then applied to the field of multiple testing procedures to explain the assumption of monotonicity of the critical constants. Lastly, we apply our model to a behavioral weight loss and maintenance study to select the optimal critical constants. Conclusions The proposed model is easy to implement and preserves the information from the alternative hypothesis.

  18. Community Violence, Protective Factors, and Adolescent Mental Health: A Profile Analysis

    Science.gov (United States)

    Copeland-Linder, Nikeea; Lambert, Sharon F.; Ialongo, Nicholas S.

    2010-01-01

    This study examined interrelationships among community violence exposure, protective factors, and mental health in a sample of urban, predominantly African American adolescents (N = 504). Latent Profile Analysis was conducted to identify profiles of adolescents based on a combination of community violence exposure, self-worth, parental monitoring,…

  19. ASSESSMENT OF BACTERIAL BIOSURFACTANT PRODUCTION THROUGH AXISYMMETRICAL DROP SHAPE-ANALYSIS BY PROFILE

    NARCIS (Netherlands)

    VANDERVEGT, W; VANDERMEI, HC; BUSSCHER, HJ

    Axisymmetric drop shape analysis by profile (ADSA-P) is a technique developed in colloid and surface science to simultaneously determine the contact angle and liquid surface tension from the profile of a droplet resting on a solid surface. In this paper is described how ADSA-P can be employed to

  20. An analysis of reading profiles of first-year students at Potchefstroom ...

    African Journals Online (AJOL)

    An analysis of the reading assessment profiles of a group of first-year students at Potchefstroom University indicated that these students experienced problems across all aspects of the reading process (i.e. vocabulary, fluency, reading comprehension, and reading strategy use). The reading assessment profiles of an ...

  1. Perioperative outcomes for pediatric neurosurgical procedures: analysis of the National Surgical Quality Improvement Program-Pediatrics.

    Science.gov (United States)

    Kuo, Benjamin J; Vissoci, Joao Ricardo N; Egger, Joseph R; Smith, Emily R; Grant, Gerald A; Haglund, Michael M; Rice, Henry E

    2017-03-01

    OBJECTIVE Existing studies have shown a high overall rate of adverse events (AEs) following pediatric neurosurgical procedures. However, little is known regarding the morbidity of specific procedures or the association with risk factors to help guide quality improvement (QI) initiatives. The goal of this study was to describe the 30-day mortality and AE rates for pediatric neurosurgical procedures by using the American College of Surgeons (ACS) National Surgical Quality Improvement Program-Pediatrics (NSQIP-Peds) database platform. METHODS Data on 9996 pediatric neurosurgical patients were acquired from the 2012-2014 NSQIP-Peds participant user file. Neurosurgical cases were analyzed by the NSQIP-Peds targeted procedure categories, including craniotomy/craniectomy, defect repair, laminectomy, shunts, and implants. The primary outcome measure was 30-day mortality, with secondary outcomes including individual AEs, composite morbidity (all AEs excluding mortality and unplanned reoperation), surgical-site infection, and unplanned reoperation. Univariate analysis was performed between individual AEs and patient characteristics using Fischer's exact test. Associations between individual AEs and continuous variables (duration from admission to operation, work relative value unit, and operation time) were examined using the Student t-test. Patient characteristics and continuous variables associated with any AE by univariate analysis were used to develop category-specific multivariable models through backward stepwise logistic regression. RESULTS The authors analyzed 3383 craniotomy/craniectomy, 242 defect repair, 1811 laminectomy, and 4560 shunt and implant cases and found a composite overall morbidity of 30.2%, 38.8%, 10.2%, and 10.7%, respectively. Unplanned reoperation rates were highest for defect repair (29.8%). The mortality rate ranged from 0.1% to 1.2%. Preoperative ventilator dependence was a significant predictor of any AE for all procedure groups, whereas

  2. Determination of endpoint of procedure for radix rehmanniae steamed based on ultraviolet spectrophotometry combination with continuous wavelet transform and kernel independent component analysis.

    Science.gov (United States)

    Wang, Guoqing; Hou, Zhenyu; Tang, Yanxia; Zhao, Jianbo; Sun, Yu'an; Dong, Chunhong; Fu, Dexue

    2010-10-29

    A method for determination of the endpoint of the procedure for radix rehmanniae steamed was proposed based on UV spectrophotometry combination with continuous wavelet transform and kernel independent component analysis (UV-CWT-KICA). In the proposed method, the raw UV spectra of the rehmanniae samples during steamed procedure were measured. The raw UV spectral data were firstly pretreated by CWT for elimination of the noise signal and enrichment of the spectral resolution, then the independent components (ICs) were estimated from the mixed CWT coefficient matrix. The results show that the ICs are chemical significance with their relative concentrations gradually decreasing or increasing during the first steamed period, and the endpoint of the steamed procedure can be determined by inspection of the relative concentration profiles, at which the ICs should be approached maximum or minimum. Furthermore, the estimated ICs of rehmanniae samples from different areas or with different grades are similar, and the relative concentration of the similar ICs in different groups are increasing or decreasing before the first 14 h, and nearly steady or some decreasing after 16 h. Based on the variations of the relative concentration profiles of the ICs, the endpoint of the steamed procedure can be determined as 15 h, while that determined by sensory analysis is 14-20 h. The proposed UV-CWT-KICA method can avoid the higher deviations of the endpoints that were determined by sensory analysis. It provides an alternative approach for determination of the endpoint of the procedure for processing traditional Chinese medicine (TCM). Copyright © 2010 Elsevier B.V. All rights reserved.

  3. Meta-analysis of Cancer Gene Profiling Data.

    Science.gov (United States)

    Roy, Janine; Winter, Christof; Schroeder, Michael

    2016-01-01

    The simultaneous measurement of thousands of genes gives the opportunity to personalize and improve cancer therapy. In addition, the integration of meta-data such as protein-protein interaction (PPI) information into the analyses helps in the identification and prioritization of genes from these screens. Here, we describe a computational approach that identifies genes prognostic for outcome by combining gene profiling data from any source with a network of known relationships between genes.

  4. Estimating the Cost of Neurosurgical Procedures in a Low-Income Setting: An Observational Economic Analysis.

    Science.gov (United States)

    Abdelgadir, Jihad; Tran, Tu; Muhindo, Alex; Obiga, Doomwin; Mukasa, John; Ssenyonjo, Hussein; Muhumza, Michael; Kiryabwire, Joel; Haglund, Michael M; Sloan, Frank A

    2017-05-01

    There are no data on cost of neurosurgery in low-income and middle-income countries. The objective of this study was to estimate the cost of neurosurgical procedures in a low-resource setting to better inform resource allocation and health sector planning. In this observational economic analysis, microcosting was used to estimate the direct and indirect costs of neurosurgical procedures at Mulago National Referral Hospital (Kampala, Uganda). During the study period, October 2014 to September 2015, 1440 charts were reviewed. Of these patients, 434 had surgery, whereas the other 1006 were treated nonsurgically. Thirteen types of procedures were performed at the hospital. The estimated mean cost of a neurosurgical procedure was $542.14 (standard deviation [SD], $253.62). The mean cost of different procedures ranged from $291 (SD, $101) for burr hole evacuations to $1,221 (SD, $473) for excision of brain tumors. For most surgeries, overhead costs represented the largest proportion of the total cost (29%-41%). This is the first study using primary data to determine the cost of neurosurgery in a low-resource setting. Operating theater capacity is likely the binding constraint on operative volume, and thus, investing in operating theaters should achieve a higher level of efficiency. Findings from this study could be used by stakeholders and policy makers for resource allocation and to perform economic analyses to establish the value of neurosurgery in achieving global health goals. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. Domain-oriented functional analysis based on expression profiling

    Directory of Open Access Journals (Sweden)

    Greene Jonathan

    2002-10-01

    Full Text Available Abstract Background Co-regulation of genes may imply involvement in similar biological processes or related function. Many clusters of co-regulated genes have been identified using microarray experiments. In this study, we examined co-regulated gene families using large-scale cDNA microarray experiments on the human transcriptome. Results We present a simple model, which, for each probe pair, distills expression changes into binary digits and summarizes the expression of multiple members of a gene family as the Family Regulation Ratio. The set of Family Regulation Ratios for each protein family across multiple experiments is called a Family Regulation Profile. We analyzed these Family Regulation Profiles using Pearson Correlation Coefficients and derived a network diagram portraying relationships between the Family Regulation Profiles of gene families that are well represented on the microarrays. Our strategy was cross-validated with two randomly chosen data subsets and was proven to be a reliable approach. Conclusion This work will help us to understand and identify the functional relationships between gene families and the regulatory pathways in which each family is involved. Concepts presented here may be useful for objective clustering of protein functions and deriving a comprehensive protein interaction map. Functional genomic approaches such as this may also be applicable to the elucidation of complex genetic regulatory networks.

  6. MODELLING AND VIBRATION ANALYSIS OF A ROAD PROFILE MEASURING SYSTEM

    Directory of Open Access Journals (Sweden)

    C. B. Patel

    2010-06-01

    Full Text Available During a vehicle development program, load data representing severe customer usage is required. The dilemma faced by a design engineer during the design process is that during the initial stage, only predicted loads estimated from historical targets are available, whereas the actual loads are available only at the fag end of the process. At the same time, changes required, if any, are easier and inexpensive during the initial stages of the design process whereas they are extremely costly in the latter stages of the process. The use of road profiles and vehicle models to predict the load acting on the whole vehicle is currently being researched. This work hinges on the ability to accurately measure road profiles. The objective of the work is to develop an algorithm, using MATLAB Simulink software, to convert the input signals into measured road profile. The algorithm is checked by the MATLAB Simulink 4 degrees of freedom half car model. To make the whole Simulink model more realistic, accelerometer and laser sensor properties are introduced. The present work contains the simulation of the mentioned algorithm with a half car model and studies the results in distance, time, and the frequency domain.

  7. Analysis of protein profiles using fuzzy clustering methods

    DEFF Research Database (Denmark)

    Karemore, Gopal Raghunath; Ukendt, Sujatha; Rai, Lavanya

    The tissue protein profiles of healthy volunteers and volunteers with cervical cancer were recorded using High Performance Liquid Chromatography combined with Laser Induced Fluorescence  technique  (HPLC-LIF)  developed  in  our  lab.      We analyzed      the protein profile data using different...... clustering methods for their classification followed by various validation  measures.    The  clustering  algorithms  used  for  the  study  were  K-  means,  K- medoid, Fuzzy C-means, Gustafson-Kessel, and Gath-Geva.  The results presented in this study  conclude  that  the  protein  profiles  of  tissue......  samples  recorded  by  using  the  HPLC- LIF  system  and  the  data  analyzed  by  clustering  algorithms  quite  successfully  classifies them as belonging from normal and malignant conditions....

  8. Industrial survey of core handling, storage, and analysis procedures for ROS measurements. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Ward, C.E.; Barnwell, J.L.

    1980-10-01

    Core analysis is an essential function in the evaluation of residual oil saturation of a reservoir. This report describes the basic techniques of core handling and measurement of porosity, permeability, and fluid saturations. Special analysis techniques such as wettability, imbibition, capillary pressure and waterflood tests are also described. Interviews with industry experts provided opinions on the applicability and reliability of the various alternative testing procedures as well as an estimation of the size of the US domestic market in coring and core analysis services.

  9. Digital analysis of cDNA abundance; expression profiling by means of restriction fragment fingerprinting

    Directory of Open Access Journals (Sweden)

    Regenbogen Johannes

    2002-03-01

    Full Text Available Abstract Background Gene expression profiling among different tissues is of paramount interest in various areas of biomedical research. We have developed a novel method (DADA, Digital Analysis of cDNA Abundance, that calculates the relative abundance of genes in cDNA libraries. Results DADA is based upon multiple restriction fragment length analysis of pools of clones from cDNA libraries and the identification of gene-specific restriction fingerprints in the resulting complex fragment mixtures. A specific cDNA cloning vector had to be constructed that governed missing or incomplete cDNA inserts which would generate misleading fingerprints in standard cloning vectors. Double stranded cDNA was synthesized using an anchored oligo dT primer, uni-directionally inserted into the DADA vector and cDNA libraries were constructed in E. coli. The cDNA fingerprints were generated in a PCR-free procedure that allows for parallel plasmid preparation, labeling, restriction digest and fragment separation of pools of 96 colonies each. This multiplexing significantly enhanced the throughput in comparison to sequence-based methods (e.g. EST approach. The data of the fragment mixtures were integrated into a relational database system and queried with fingerprints experimentally produced by analyzing single colonies. Due to limited predictability of the position of DNA fragments on the polyacrylamid gels of a given size, fingerprints derived solely from cDNA sequences were not accurate enough to be used for the analysis. We applied DADA to the analysis of gene expression profiles in a model for impaired wound healing (treatment of mice with dexamethasone. Conclusions The method proved to be capable of identifying pharmacologically relevant target genes that had not been identified by other standard methods routinely used to find differentially expressed genes. Due to the above mentioned limited predictability of the fingerprints, the method was yet tested only with

  10. The effects of post-persulfate-digestion procedures on total phosphorus analysis in water.

    Science.gov (United States)

    Zhou, Meifang; Struve, David M

    2004-11-01

    There are differences between the EPA Method 365 and the APHA-AWWA-WEF's Standard Method 4500 with respect to the post-digestion treatment procedures of the persulfate-digested water. The effects on total phosphorus analysis of different post-digestion treatment procedures, such as neutralization and reacidification, and shaking/settling, were investigated in this study using the total phosphorus measurements of water samples from the Everglades Round Robin (ERR) study and comparing the results with the ERR study. The effects of the insoluble particles or phosphorus adsorption/precipitation on/with Al and Fe hydroxides in different post-digestion treatment procedures adequately accounted for the differences between the most probable value and the higher or lower total phosphorus measurements reported in the ERR study. Based on the results of this investigation we recommend that a clearly defined set of digestion and post-digestion treatment procedures be adopted as the standard for total phosphorus analysis using the ascorbic acid method.

  11. Procedure for statistical analysis of one-parameter discrepant experimental data.

    Science.gov (United States)

    Badikov, Sergey A; Chechev, Valery P

    2012-09-01

    A new, Mandel-Paule-type procedure for statistical processing of one-parameter discrepant experimental data is described. The procedure enables one to estimate a contribution of unrecognized experimental errors into the total experimental uncertainty as well as to include it in analysis. A definition of discrepant experimental data for an arbitrary number of measurements is introduced as an accompanying result. In the case of negligible unrecognized experimental errors, the procedure simply reduces to the calculation of the weighted average and its internal uncertainty. The procedure was applied to the statistical analysis of half-life experimental data; Mean half-lives for 20 actinides were calculated and results were compared to the ENSDF and DDEP evaluations. On the whole, the calculated half-lives are consistent with the ENSDF and DDEP evaluations. However, the uncertainties calculated in this work essentially exceed the ENSDF and DDEP evaluations for discrepant experimental data. This effect can be explained by adequately taking into account unrecognized experimental errors. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. Forensic analysis of Salvia divinorum using multivariate statistical procedures. Part I: discrimination from related Salvia species.

    Science.gov (United States)

    Willard, Melissa A Bodnar; McGuffin, Victoria L; Smith, Ruth Waddell

    2012-01-01

    Salvia divinorum is a hallucinogenic herb that is internationally regulated. In this study, salvinorin A, the active compound in S. divinorum, was extracted from S. divinorum plant leaves using a 5-min extraction with dichloromethane. Four additional Salvia species (Salvia officinalis, Salvia guaranitica, Salvia splendens, and Salvia nemorosa) were extracted using this procedure, and all extracts were analyzed by gas chromatography-mass spectrometry. Differentiation of S. divinorum from other Salvia species was successful based on visual assessment of the resulting chromatograms. To provide a more objective comparison, the total ion chromatograms (TICs) were subjected to principal components analysis (PCA). Prior to PCA, the TICs were subjected to a series of data pretreatment procedures to minimize non-chemical sources of variance in the data set. Successful discrimination of S. divinorum from the other four Salvia species was possible based on visual assessment of the PCA scores plot. To provide a numerical assessment of the discrimination, a series of statistical procedures such as Euclidean distance measurement, hierarchical cluster analysis, Student's t tests, Wilcoxon rank-sum tests, and Pearson product moment correlation were also applied to the PCA scores. The statistical procedures were then compared to determine the advantages and disadvantages for forensic applications.

  13. Assessment of ecological risks at former landfill site using TRIAD procedure and multicriteria analysis.

    Science.gov (United States)

    Sorvari, Jaana; Schultz, Eija; Haimi, Jari

    2013-02-01

    Old industrial landfills are important sources of environmental contamination in Europe, including Finland. In this study, we demonstrated the combination of TRIAD procedure, multicriteria decision analysis (MCDA), and statistical Monte Carlo analysis for assessing the risks to terrestrial biota in a former landfill site contaminated by petroleum hydrocarbons (PHCs) and metals. First, we generated hazard quotients by dividing the concentrations of metals and PHCs in soil by the corresponding risk-based ecological benchmarks. Then we conducted ecotoxicity tests using five plant species, earthworms, and potworms, and determined the abundance and diversity of soil invertebrates from additional samples. We aggregated the results in accordance to the methods used in the TRIAD procedure, conducted rating of the assessment methods based on their performance in terms of specific criteria, and weighted the criteria using two alternative weighting techniques to produce performance scores for each method. We faced problems in using the TRIAD procedure, for example, the results from the animal counts had to be excluded from the calculation of integrated risk estimates (IREs) because our reference soil sample showed the lowest biodiversity and abundance of soil animals. In addition, hormesis hampered the use of the results from the ecotoxicity tests. The final probabilistic IREs imply significant risks at all sampling locations. Although linking MCDA with TRIAD provided a useful means to study and consider the performance of the alternative methods in predicting ecological risks, some uncertainties involved still remained outside the quantitative analysis. © 2012 Society for Risk Analysis.

  14. Characterization of plasma metal profiles in Alzheimer's disease using multivariate statistical analysis

    National Research Council Canada - National Science Library

    Chunmei Guan; Rui Dang; Yu Cui; Liyan Liu; Xiaobei Chen; Xiaoyu Wang; Jingli Zhu; Donggang Li; Junwei Li; Decai Wang

    .... We have used an analytical approach, based on inductively coupled plasma mass spectrometry coupled with multivariate statistical analysis, to study the profiles of a wide range of metals in AD...

  15. Characterization of plasma metal profiles in Alzheimer’s disease using multivariate statistical analysis

    National Research Council Canada - National Science Library

    Chunmei Guan; Rui Dang; Yu Cui; Liyan Liu; Xiaobei Chen; Xiaoyu Wang; Jingli Zhu; Donggang Li; Junwei Li; Decai Wang

    2017-01-01

    .... We have used an analytical approach, based on inductively coupled plasma mass spectrometry coupled with multivariate statistical analysis, to study the profiles of a wide range of metals in AD...

  16. Motor vehicle trauma: analysis of injury profiles by road-user category

    National Research Council Canada - National Science Library

    Markogiannakis, H; Sanidas, E; Messaris, E; Koutentakis, D; Alpantaki, K; Kafetzakis, A; Tsiftsis, D

    2006-01-01

    .... The mechanism of injury influences the patterns of injury in victims of vehicle accidents. Identification and analysis of injury profiles of motor-vehicle trauma patients in a Greek level I trauma centre, by road-user category...

  17. Co-occurring posttraumatic stress and depression symptoms after sexual assault: a latent profile analysis

    National Research Council Canada - National Science Library

    Au, Teresa M; Dickstein, Benjamin D; Comer, Jonathan S; Salters-Pedneault, Kristalyn; Litz, Brett T

    2013-01-01

    ... meaningfully distinct subgroups with discordant PTSD and depression symptoms. Latent profile analysis was used to examine self-reported PTSD and depression symptoms among 119 female sexual assault survivors at 1-, 2-, 3-, and 4-months post-assault...

  18. Automated 3D analysis of pre-procedural MDCT to predict annulus plane angulation and C-arm positioning: benefit on procedural outcome in patients referred for TAVR.

    Science.gov (United States)

    Samim, Mariam; Stella, Pieter R; Agostoni, Pierfrancesco; Kluin, Jolanda; Ramjankhan, Faiez; Budde, Ricardo P J; Sieswerda, Gertjan; Algeri, Emanuela; van Belle, Camille; Elkalioubie, Ahmed; Juthier, Francis; Belkacemi, Anouar; Bertrand, Michel E; Doevendans, Pieter A; Van Belle, Eric

    2013-02-01

    The aim of this study was to determine whether pre-procedural analysis of multidetector row computed tomography (MDCT) scans could accurately predict the "line of perpendicularity" (LP) of the aortic annulus and corresponding C-arm angulations required for prosthesis delivery and impact the outcome of the procedure. Optimal positioning of the transcatheter aortic prosthesis is paramount to transcatheter aortic valve replacement (TAVR) procedural success. All patients referred for TAVR at our center underwent a routine pre-procedural MDCT scan. A 3-dimensional (3D) analysis using software dedicated to define the LP of the aortic annulus and the corresponding C-arm positioning was performed in 71 consecutive patients. In 35 patients, the results of the MDCT analysis were not available at the time of the procedure (angiography cohort). In that cohort the position of the C-arm was determined during the procedure using ad-hoc angiography. In 36 patients, the MDCT analysis was performed pre-procedure and results were available at the time of the procedure (MDCT cohort). In that cohort the position of the C-arm was derived from the MDCT analysis rather than by ad-hoc angiography. Intraobserver and interobserver reproducibility of MDCT analysis to predict the LP of the aortic annulus were excellent (kappa = 1 and 0.94, respectively). Patient variations of the LP ranged >70°. Compared with the angiography cohort, the MDCT cohort was associated with a significant decrease in implantation time (p = 0.0001), radiation exposure (p = 0.02), amount of contrast (p = 0.001), and risk of acute kidney injury (p = 0.03). Additionally, the combined rate of valve malposition and aortic regurgitation was also reduced (6% vs. 23%, p = 0.03). Automated 3D analysis of pre-implantation MDCT accurately predicts the LP of the aortic annulus and the corresponding C-arm position required for TAVR. With this approach, the implantation of the balloon-expandable prosthetic valve can be performed

  19. Numerical procedure for the vibration analysis of arbitrarily constrained stiffened panels with openings

    Directory of Open Access Journals (Sweden)

    Dae Seung Cho

    2014-12-01

    Full Text Available A simple and efficient vibration analysis procedure for stiffened panels with openings and arbitrary boundary conditions based on the assumed mode method is presented. Natural frequencies and modes are determined by solving an eigenvalue problem of a multi-degree-of-freedom system matrix equation derived by using Lagrange's equations of motion, where Mindlin theory is applied for plate and Timoshenko beam theory for stiffeners. The effect of stiffeners on vibration response is taken into account by adding their strain and kinetic energies to the corresponding plate energies whereas the strain and kinetic energies of openings are subtracted from the plate energies. Different stiffened panels with various opening shapes and dispositions for several combinations of boundary conditions are analyzed and the results show good agreement with those obtained by the finite element analysis. Hence, the proposed procedure is especially appropriate for use in the preliminary design stage of stiffened panels with openings.

  20. Clustering Techniques in Load Profile Analysis for Distribution Stations

    Directory of Open Access Journals (Sweden)

    BOBRIC, E. C.

    2009-02-01

    Full Text Available The demand characteristic is the most important one in analyzing customer information. In a distribution network, there is in any moment certain degree of uncertainty about busses loads, and consequently, about load level of network, busses voltage level, and power losses. Therefore, it is very important to estimate first of all the load profiles of buses, using available data (measurements effectuated in distribution stations. The results obtained for various distribution stations demonstrate the effectiveness of the present method in overcoming the difficulties encountered in optimal planning and operation of distribution networks.

  1. Wave Concept Iterative Procedure Analysis of Patch Antennas on Nanostructured Ceramic Substrates

    Directory of Open Access Journals (Sweden)

    V. Silva Neto

    2014-02-01

    Full Text Available The wave concept iterative procedure (WCIP is proposed to analyze rectangular and circular patch antennas on nanostructured ceramic substrates. Principles of WCIP are described and advantages are emphasized. The analysis of microstrip antennas on double layered substrates is performed in space and spectral domains. In addition, Fast Fourier Transformation (FFT is used to improve the efficiency of the method. WCIP simulated results are compared to HFSS software ones. A good agreement is observed.

  2. Staged versus index procedure complete revascularization in ST-elevation myocardial infarction: A meta-analysis.

    Science.gov (United States)

    Agarwal, Nayan; Jain, Ankur; Garg, Jalaj; Mojadidi, Mohammad Khalid; Mahmoud, Ahmed N; Patel, Nimesh Kirit; Agrawal, Sahil; Gupta, Tanush; Bhatia, Nirmanmoh; Anderson, R David

    2017-10-01

    Complete revascularization of patients with ST-elevation myocardial infarction and multivessel coronary artery disease reduces adverse events compared to infarct-related artery only revascularization. Whether complete revascularization should be done as multivessel intervention during index procedure or as a staged procedure remains controversial. We performed a meta-analysis of randomized controlled trials comparing outcomes of multivessel intervention in patients with ST-elevation myocardial infarction and multivessel coronary artery disease as staged procedure versus at the time of index procedure. Composite of death or myocardial infarction was the primary outcome. Mantel-Haenszel risk ratios were calculated using random effect model. Six randomized studies with a total of 1126 patients met our selection criteria. At a mean follow-up of 13 months, composite of myocardial infarction or death (7.2% vs 11.7%, RR: 1.66, 95%CI: 1.09-2.52, P = 0.02), all cause mortality (RR: 2.55, 95%CI: 1.42-4.58, P revascularization. There was no difference in major adverse cardiac events (RR: 1.14, 95%CI: 0.88-1.49, P = 0.33), repeat myocardial infarction (RR: 1.14, 95%CI: 0.68-1.92, P = 0.61), and repeat revascularization (RR: 0.92, 95%CI: 0.66-1.28, P = 0.62). In patients with ST-elevation myocardial infarction and multivessel coronary artery disease, a strategy of complete revascularization as a staged procedure compared to index procedure revascularization results in reduced mortality without an increase in repeat myocardial infarction or need for repeat revascularization. © 2017, Wiley Periodicals, Inc.

  3. Analysis of chemical profiles of insect adhesion secretions by gas chromatography–mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Reitz, Manuela [Institute of Organic Chemistry, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen (Germany); Gerhardt, Heike [Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen (Germany); Schmitt, Christian; Betz, Oliver [Institute of Evolution and Ecology, University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen (Germany); Albert, Klaus, E-mail: klaus.albert@uni-tuebingen.de [Institute of Organic Chemistry, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen (Germany); Lämmerhofer, Michael, E-mail: michael.laemmerhofer@uni-tuebingen.de [Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen (Germany)

    2015-01-07

    Highlights: • Adhesion secretions of desert locust analyzed by GC–MS. • Insect secretions are composed of apolar and polar constituents. • Sampling simplified with contact SPME as compared to solvent sampling. • Thin-film SPME-GC–MS revealed complex alkane patterns for insect secretions. • Differences in tarsal (feet) secretions and samples from tibiae (upper legs) identified. - Abstract: This article reports on the chemical analysis of molecular profiles of tarsal secretions of the desert locust Schistocerca gregaria (Forsskål, 1775) by gas chromatography hyphenated with quadrupol mass spectrometry (GC–MS) as well as {sup 1}H-nuclear magnetic resonance ({sup 1}H NMR) spectroscopy. Special focus of this study was to elaborate on sampling methods which enable selective microscale extraction of insect secretions in a spatially controlled manner, in particular tarsal adhesive secretions and secretions located on cuticle surfaces at the tibia. Various solvent sampling procedures and contact solid-phase microextraction (SPME) methods were compared in terms of comprehensiveness and extraction efficiencies as measured by signal intensities in GC–MS. Solvent sampling with water as extraction solvent gave access to the elucidation of chemical profiles of polar compound classes such as amino acids and carbohydrates, but is extremely tedious. Contact SPME on the other hand can be regarded as a simplified and more elegant alternative, in particular for the lipophilic compound fraction. Many proteinogenic amino acids and ornithine as well as carbohydrate monomers arabinose, xylose, glucose, and galactose were detected in tarsal secretions after acid hydrolysis of aqueous extracts. Qualitatively similar but quantitatively significantly different molecular profiles were found for the lipid fraction which contained mainly n-alkanes and internally branched monomethyl-, dimethyl-, and trimethyl-alkanes in the C23–C49 range as well as long chain fatty acids and

  4. Meta-analysis of the technical performance of an imaging procedure: Guidelines and statistical methodology

    Science.gov (United States)

    Huang, Erich P; Wang, Xiao-Feng; Choudhury, Kingshuk Roy; McShane, Lisa M; Gönen, Mithat; Ye, Jingjing; Buckler, Andrew J; Kinahan, Paul E; Reeves, Anthony P; Jackson, Edward F; Guimaraes, Alexander R; Zahlmann, Gudrun

    2017-01-01

    Medical imaging serves many roles in patient care and the drug approval process, including assessing treatment response and guiding treatment decisions. These roles often involve a quantitative imaging biomarker, an objectively measured characteristic of the underlying anatomic structure or biochemical process derived from medical images. Before a quantitative imaging biomarker is accepted for use in such roles, the imaging procedure to acquire it must undergo evaluation of its technical performance, which entails assessment of performance metrics such as repeatability and reproducibility of the quantitative imaging biomarker. Ideally, this evaluation will involve quantitative summaries of results from multiple studies to overcome limitations due to the typically small sample sizes of technical performance studies and/or to include a broader range of clinical settings and patient populations. This paper is a review of meta-analysis procedures for such an evaluation, including identification of suitable studies, statistical methodology to evaluate and summarize the performance metrics, and complete and transparent reporting of the results. This review addresses challenges typical of meta-analyses of technical performance, particularly small study sizes, which often causes violations of assumptions underlying standard meta-analysis techniques. Alternative approaches to address these difficulties are also presented; simulation studies indicate that they outperform standard techniques when some studies are small. The meta-analysis procedures presented are also applied to actual [18F]-fluorodeoxyglucose positron emission tomography (FDG-PET) test–retest repeatability data for illustrative purposes. PMID:24872353

  5. AntDAS: Automatic Data Analysis Strategy for UPLC-QTOF-Based Nontargeted Metabolic Profiling Analysis.

    Science.gov (United States)

    Fu, Hai-Yan; Guo, Xiao-Ming; Zhang, Yue-Ming; Song, Jing-Jing; Zheng, Qing-Xia; Liu, Ping-Ping; Lu, Peng; Chen, Qian-Si; Yu, Yong-Jie; She, Yuanbin

    2017-10-17

    High-quality data analysis methodology remains a bottleneck for metabolic profiling analysis based on ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry. The present work aims to address this problem by proposing a novel data analysis strategy wherein (1) chromatographic peaks in the UPLC-QTOF data set are automatically extracted by using an advanced multiscale Gaussian smoothing-based peak extraction strategy; (2) a peak annotation stage is used to cluster fragment ions that belong to the same compound. With the aid of high-resolution mass spectrometer, (3) a time-shift correction across the samples is efficiently performed by a new peak alignment method; (4) components are registered by using a newly developed adaptive network searching algorithm; (5) statistical methods, such as analysis of variance and hierarchical cluster analysis, are then used to identify the underlying marker compounds; finally, (6) compound identification is performed by matching the extracted peak information, involving high-precision m/z and retention time, against our compound library containing more than 500 plant metabolites. A manually designed mixture of 18 compounds is used to evaluate the performance of the method, and all compounds are detected under various concentration levels. The developed method is comprehensively evaluated by an extremely complex plant data set containing more than 2000 components. Results indicate that the performance of the developed method is comparable with the XCMS. The MATLAB GUI code is available from http://software.tobaccodb.org/software/antdas .

  6. Ingredient classification according to the digestible amino acid profile: an exploratory analysis

    Directory of Open Access Journals (Sweden)

    DE Faria Filho

    2005-09-01

    Full Text Available This study aimed: 1 to classify ingredients according to the digestible amino acid (AA profile; 2 to determine ingredients with AA profile closer to the ideal for broiler chickens; and 3 to compare digestible AA profiles from simulated diets with the ideal protein profile. The digestible AA levels of 30 ingredients were compiled from the literature and presented as percentages of lysine according to the ideal protein concept. Cluster and principal component analyses (exploratory analyses were used to compose and describe groups of ingredients according to AA profiles. Four ingredient groups were identified by cluster analysis, and the classification of the ingredients within each of these groups was obtained from a principal component analysis, showing 11 classes of ingredients with similar digestible AA profiles. The ingredients with AA profiles closer to the ideal protein were meat and bone meal 45, fish meal 60 and wheat germ meal, all of them constituting Class 1; the ingredients from the other classes gradually diverged from the ideal protein. Soybean meal, which is the main protein source for poultry, showed good AA balance since it was included in Class 3. On the contrary, corn, which is the main energy source in poultry diets, was classified in Class 8. Dietary AA profiles were improved when corn and/or soybean meal were partially or totally replaced in the simulations by ingredients with better AA balance.

  7. Flood-profile analysis, Big Darby Creek at State Route 762, Orient, Ohio

    Science.gov (United States)

    Bartlett, W.P.; Sherwood, J.M.

    1984-01-01

    The U.S. Geological Survey, in cooperation with the Ohio Department of Transportation, made a hydraulic analysis of the 25- and 100-year floods on Big Darby Creek where State Route 762 crosses the stream at Orient, Ohio. Two proposed bridge plans were analyzed to determine the effects on flood profiles subsequent to the placement of a 376-foot-long, four-span deck bridge across the stream 90 feet upstream of the existing State Route 762 bridge. In plan 1, the brigde is set at a 25-degrees skew to the river and at sufficient elevation to pass the 100-year flood below it. In plan 2, the skew is 28 degrees and elevations are 3 feet lower than in plan 1, which result in partial submergence of the bridge during the 100-year flood. This analysis shows that the 25-year flood profiles upstream of the new bridge would increase by 0.4 foot if plan 1 is adopted and by 0.3 foot if plan 2 is adopted. Both profiles converge with the present-condition profiles 5,750 feet upstream. The profiles for the 100-year flood would increase by 0.6 foot plan 1 and 1.1 feet for plan 2. This additional backwater affects profiles is 0.1 foot higher than for present conditions, and the plan 2 profile is 0.2 foot higher than profiles for present conditions.

  8. A new method to identify the foot of continental slope based on an integrated profile analysis

    Science.gov (United States)

    Wu, Ziyin; Li, Jiabiao; Li, Shoujun; Shang, Jihong; Jin, Xiaobin

    2017-06-01

    A new method is proposed to identify automatically the foot of the continental slope (FOS) based on the integrated analysis of topographic profiles. Based on the extremum points of the second derivative and the Douglas-Peucker algorithm, it simplifies the topographic profiles, then calculates the second derivative of the original profiles and the D-P profiles. Seven steps are proposed to simplify the original profiles. Meanwhile, multiple identification methods are proposed to determine the FOS points, including gradient, water depth and second derivative values of data points, as well as the concave and convex, continuity and segmentation of the topographic profiles. This method can comprehensively and intelligently analyze the topographic profiles and their derived slopes, second derivatives and D-P profiles, based on which, it is capable to analyze the essential properties of every single data point in the profile. Furthermore, it is proposed to remove the concave points of the curve and in addition, to implement six FOS judgment criteria.

  9. Ravitch versus Nuss procedure for pectus excavatum: systematic review and meta-analysis

    Science.gov (United States)

    Kanagaratnam, Aran; Phan, Steven; Tchantchaleishvilli, Vakhtang

    2016-01-01

    Background Pectus excavatum is the most common congenital chest wall deformity. The two most common surgical techniques for its correction are the modified Ravitch technique and the minimally invasive Nuss technique. Despite both procedures being used widely, data comparing them are scarce. Methods We conducted a systematic review and meta-analysis of comparative studies to evaluate these procedures. A systematic search of the literature was performed from six electronic databases. Pooled meta-analysis was conducted using odds ratio (OR) and weighted mean difference (WMD). Results A total of 13 studies comprising 1,432 pediatric (79.3%) and adult (20.7%) patients were identified, including 912 patients undergoing the Nuss procedure compared to 520 patients undergoing the Ravitch procedure. There was no significant difference found between the Nuss group versus Ravitch group in pediatric patients with regard to overall complications (OR =1.16; 95% CI: 0.61–2.19; I2=56%; P=0.65), reoperations (6.1% vs. 6.4%; OR =1.00; 95% CI: 0.40–2.50; I2=0%; P=1.00), wound infections (OR =0.58; 95% CI: 0.23–1.46; I2=0%; P=0.25), hemothorax (1.6% vs. 1.3%; OR =0.74; 95% CI: 0.21–2.65; I2=12%; P=0.64), pneumothorax (3.4% vs. 1.5%; OR =1.11; 95% CI: 0.42–2.93; I2=0%; P=0.83) or pneumonia (OR =0.15; 95% CI: 0.02–1.48; I2=0%; P=0.10). Adult patients undergoing the Nuss procedure had a higher incidence of overall complications (OR =3.26; 95% CI: 1.01–10.46; I2=0%; P=0.05), though there were far fewer studies that reported data. Conclusions These results suggest no difference between the Nuss and Ravitch procedures for pediatric patients, while in adults the Ravitch procedure resulted in fewer complications. PMID:27747174

  10. Multi-response permutation procedure as an alternative to the analysis of variance: an SPSS implementation.

    Science.gov (United States)

    Cai, Li

    2006-02-01

    A permutation test typically requires fewer assumptions than does a comparable parametric counterpart. The multi-response permutation procedure (MRPP) is a class of multivariate permutation tests of group difference useful for the analysis of experimental data. However, psychologists seldom make use of the MRPP in data analysis, in part because the MRPP is not implemented in popular statistical packages that psychologists use. A set of SPSS macros implementing the MRPP test is provided in this article. The use of the macros is illustrated by analyzing example data sets.

  11. Patent Network Analysis and Quadratic Assignment Procedures to Identify the Convergence of Robot Technologies.

    Directory of Open Access Journals (Sweden)

    Woo Jin Lee

    Full Text Available Because of the remarkable developments in robotics in recent years, technological convergence has been active in this area. We focused on finding patterns of convergence within robot technology using network analysis of patents in both the USPTO and KIPO. To identify the variables that affect convergence, we used quadratic assignment procedures (QAP. From our analysis, we observed the patent network ecology related to convergence and found technologies that have great potential to converge with other robotics technologies. The results of our study are expected to contribute to setting up convergence based R&D policies for robotics, which can lead new innovation.

  12. Random analysis of bearing capacity of square footing using the LAS procedure

    Science.gov (United States)

    Kawa, Marek; Puła, Wojciech; Suska, Michał

    2016-09-01

    In the present paper, a three-dimensional problem of bearing capacity of square footing on random soil medium is analyzed. The random fields of strength parameters c and φ are generated using LAS procedure (Local Average Subdivision, Fenton and Vanmarcke 1990). The procedure used is re-implemented by the authors in Mathematica environment in order to combine it with commercial program. Since the procedure is still tested the random filed has been assumed as one-dimensional: the strength properties of soil are random in vertical direction only. Individual realizations of bearing capacity boundary-problem with strength parameters of medium defined the above procedure are solved using FLAC3D Software. The analysis is performed for two qualitatively different cases, namely for the purely cohesive and cohesive-frictional soils. For the latter case the friction angle and cohesion have been assumed as independent random variables. For these two cases the random square footing bearing capacity results have been obtained for the range of fluctuation scales from 0.5 m to 10 m. Each time 1000 Monte Carlo realizations have been performed. The obtained results allow not only the mean and variance but also the probability density function to be estimated. An example of application of this function for reliability calculation has been presented in the final part of the paper.

  13. Financial incentives for lumbar surgery: a critical analysis of physician reimbursement for decompression and fusion procedures.

    Science.gov (United States)

    Whang, Peter G; Lim, Moe R; Sasso, Rick C; Skelton, Alta; Brown, Zoe B; Greg Anderson, David; Albert, Todd J; Hilibrand, Alan S; Vaccaro, Alexander R

    2008-08-01

    Retrospective case-control study/economic analysis. To determine the treatment times required for isolated lumbar decompressions and for combined decompression and instrumented fusion procedures to compare the relative reimbursements for each type of operation as a function of time expenditure by the surgeon. Under current Medicare fee schedules, the payment for a fusion procedure is higher than of an isolated decompression. It has been recently suggested in the lay press that the greater reimbursement for a lumbar arthrodesis may inappropriately influence the manner in which surgeons elect to treat lumbar degenerative conditions, resulting in what they believe to be a substantial number of unnecessary spinal fusions. A consecutive series of 50 single-level decompression cases performed by single surgeon were retrospectively analyzed and compared with an equivalent cohort of subjects who underwent single-level decompression and instrumented posterolateral fusion with autogenous iliac crest bone grafting. The operative reports, office charts, and billing records were reviewed to determine the total clinical time invested by the surgeon and the Medicare reimbursement for each surgery. Relative to the corresponding values of the decompression group, combined decompression and fusion procedures were associated with a longer mean surgical time (134.6 min vs. 47.3 min, Pundue financial incentive to recommend a combined decompression and instrumented fusion procedure over an isolated decompression to patients with symptomatic lumbar degeneration, especially when considering the greater time, effort, and risk characteristic of this more complex operation.

  14. Risk analysis procedure for post-wildfire natural hazards in British Columbia

    Science.gov (United States)

    Jordan, Peter

    2010-05-01

    Following a severe wildfire season in 2003, and several subsequent damaging debris flow and flood events, the British Columbia Forest Service developed a procedure for analysing risks to public safety and infrastructure from such events. At the same time, the Forest Service undertook a research program to determine the extent of post-wildfire hazards, and examine the hydrologic and geomorphic processes contributing to the hazards. The risk analysis procedure follows the Canadian Standards Association decision-making framework for risk management (which in turn is based on international standards). This has several steps: identification of risk, risk analysis and estimation, evaluation of risk tolerability, developing control or mitigation strategies, and acting on these strategies. The Forest Service procedure deals only with the first two steps. The results are passed on to authorities such as the Provincial Emergency Program and local government, who are responsible for evaluating risks, warning residents, and applying mitigation strategies if appropriate. The objective of the procedure is to identify and analyse risks to public safety and infrastructure. The procedure is loosely based on the BAER (burned area emergency response) program in the USA, with some important differences. Our procedure focuses on identifying risks and warning affected parties, not on mitigation activities such as broadcast erosion control measures. Partly this is due to limited staff and financial resources. Also, our procedure is not multi-agency, but is limited to wildfires on provincial forest land; in British Columbia about 95% of forest land is in the publicly-owned provincial forest. Each fire season, wildfires are screened by size and proximity to values at risk such as populated areas. For selected fires, when the fire is largely contained, the procedure begins with an aerial reconnaissance of the fire, and photography with a hand-held camera, which can be used to make a

  15. Inverse procedure for high-latitude ionospheric electrodynamics: Analysis of satellite-borne magnetometer data

    Science.gov (United States)

    Matsuo, Tomoko; Knipp, Delores J.; Richmond, Arthur D.; Kilcommons, Liam; Anderson, Brian J.

    2015-06-01

    This paper presents an analysis of data from the magnetometers on board the Defense Meteorological Satellite Program (DMSP) F-15, F-16, F-17, and F-18 satellites and the Iridium satellite constellation, using an inverse procedure for high-latitude ionospheric electrodynamics, during the period of 29-30 May 2010. The Iridium magnetometer data are made available through the Active Magnetosphere and Planetary Electrodynamics Response Experiment (AMPERE) program. The method presented here is built upon the assimilative mapping of ionospheric electrodynamics procedure but with a more complete treatment of the prior model uncertainty to facilitate an optimal inference of complete polar maps of electrodynamic variables from irregularly distributed observational data. The procedure can provide an objective measure of uncertainty associated with the analysis. The cross-validation analysis, in which the DMSP data are used as independent validation data sets, suggests that the procedure yields the spatial prediction of DMSP perturbation magnetic fields from AMPERE data alone with a median discrepancy of 30-50 nT. Discrepancies larger than 100 nT are seen in about 20% of total samples, whose location and magnitude are generally consistent with the previously identified discrepancy between DMSP and AMPERE data sets. Resulting field-aligned current (FAC) patterns exhibit more distinct spatial patterns without spurious high-frequency oscillatory features in comparison to the FAC products provided by AMPERE. Maps of the toroidal magnetic potential and FAC estimated from both AMPERE and DMSP data under four distinctive interplanetary magnetic field (IMF) conditions during a magnetic cloud event demonstrate the IMF control of high-latitude electrodynamics and the opportunity for future scientific investigation.

  16. Dangers resulting from DNA profiling of biological materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT with regard to forensic genetic analysis

    Directory of Open Access Journals (Sweden)

    Renata Jacewicz

    2016-07-01

    Full Text Available The study documents the risk that comes with DNA analysis of materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT in forensic genetics. DNA chimerism was studied in 30 patients after allo-HSCT, based on techniques applied in contemporary forensic genetics, i.e. real-time PCR and multiplex PCR-STR with the use of autosomal DNA as well as Y-DNA markers. The results revealed that the DNA profile of the recipient’s blood was identical with the donor’s in the majority of cases. Therefore, blood analysis can lead to false conclusions in personal identification as well as kinship analysis. An investigation of buccal swabs revealed a mixture of DNA in the majority of recipients. Consequently, personal identification on the basis of stain analysis of the same origin may be impossible. The safest (but not ideal material turned out to be the hair root. Its analysis based on autosomal DNA revealed 100% of the recipient’s profile. However, an analysis based on Y-chromosome markers performed in female allo-HSCT recipients with male donors demonstrated the presence of donor DNA in hair cells – similarly to the blood and buccal swabs. In the light of potential risks arising from DNA profiling of biological materials derived from persons after allotransplantation in judicial aspects, certain procedures were proposed to eliminate such dangers. The basic procedures include abandoning the approach based exclusively on blood collection, both for kinship analysis and personal identification; asking persons who are to be tested about their history of allo-HSCT before sample collection and profile entry in the DNA database, and verification of DNA profiling based on hair follicles in uncertain cases.

  17. Dangers resulting from DNA profiling of biological materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) with regard to forensic genetic analysis.

    Science.gov (United States)

    Jacewicz, R; Lewandowski, K; Rupa-Matysek, J; Jędrzejczyk, M; Berent, J

    The study documents the risk that comes with DNA analysis of materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in forensic genetics. DNA chimerism was studied in 30 patients after allo-HSCT, based on techniques applied in contemporary forensic genetics, i.e. real-time PCR and multiplex PCR-STR with the use of autosomal DNA as well as Y-DNA markers. The results revealed that the DNA profile of the recipient's blood was identical with the donor's in the majority of cases. Therefore, blood analysis can lead to false conclusions in personal identification as well as kinship analysis. An investigation of buccal swabs revealed a mixture of DNA in the majority of recipients. Consequently, personal identification on the basis of stain analysis of the same origin may be impossible. The safest (but not ideal) material turned out to be the hair root. Its analysis based on autosomal DNA revealed 100% of the recipient's profile. However, an analysis based on Y-chromosome markers performed in female allo-HSCT recipients with male donors demonstrated the presence of donor DNA in hair cells - similarly to the blood and buccal swabs. In the light of potential risks arising from DNA profiling of biological materials derived from persons after allotransplantation in judicial aspects, certain procedures were proposed to eliminate such dangers. The basic procedures include abandoning the approach based exclusively on blood collection, both for kinship analysis and personal identification; asking persons who are to be tested about their history of allo-HSCT before sample collection and profile entry in the DNA database, and verification of DNA profiling based on hair follicles in uncertain cases.

  18. Determining the optimal forensic DNA analysis procedure following investigation of sample quality.

    Science.gov (United States)

    Hedell, Ronny; Hedman, Johannes; Mostad, Petter

    2017-07-17

    Crime scene traces of various types are routinely sent to forensic laboratories for analysis, generally with the aim of addressing questions about the source of the trace. The laboratory may choose to analyse the samples in different ways depending on the type and quality of the sample, the importance of the case and the cost and performance of the available analysis methods. Theoretically well-founded guidelines for the choice of analysis method are, however, lacking in most situations. In this paper, it is shown how such guidelines can be created using Bayesian decision theory. The theory is applied to forensic DNA analysis, showing how the information from the initial qPCR analysis can be utilized. It is assumed the alternatives for analysis are using a standard short tandem repeat (STR) DNA analysis assay, using the standard assay and a complementary assay, or the analysis may be cancelled following quantification. The decision is based on information about the DNA amount and level of DNA degradation of the forensic sample, as well as case circumstances and the cost for analysis. Semi-continuous electropherogram models are used for simulation of DNA profiles and for computation of likelihood ratios. It is shown how tables and graphs, prepared beforehand, can be used to quickly find the optimal decision in forensic casework.

  19. [Profile and procedures of the community health agents regarding oral health in the countryside of Piauí State, Brazil].

    Science.gov (United States)

    Moura, Marcoeli Silva de; Carvalho, Claro José de; Amorim, José Tadeu Cavalcante de; Marques, Maurílio Francisco Soares Siqueira; Moura, Lúcia de Fátima Almeida de Deus; Mendes, Regina Ferraz

    2010-06-01

    The aim of this survey was to draw the demographic profile and the actions concerning oral health carried out by the communitarian agents of health, in small towns of Piauí State, Brazil. Four towns were chosen: Agua Branca, Piracuruca, Queimada Nova e Simões. The method used was observational descriptive cross-sectional. One hundred and nine agents were assessed through questionnaires, with 28 multiples choice questions. Data was analyzed in Excel. It was possible to conclude that: the communitarian agents on the countryside of Piauí are predominantly females, married, age between 20-39, with one to three children, nine years of formal education, monthly income of one minimum salary, and has been living in their community for 24 years in average; the large majority of the agents has not received specific qualification to carry out their job, has not attended lectures on oral health, although almost half of them works on activities related to oral health, despite not registering them properly; they perceive themselves as having a fair knowledge of oral health, which coincided with the assessment collected by the questionnaire.

  20. Microarray Analysis of the Gene Expression Profile in Triethylene ...

    African Journals Online (AJOL)

    2018-01-24

    Jan 24, 2018 ... Data were analyzed using the Expression Console and. Transcriptome Analysis Console/Partek Genomic Suite. Raw data were normalized by the robust multiarray average algorithm. Array data were filtered by detection. P < 0.05. A comparative analysis between each sample was carried out using ...

  1. Effect of music in endoscopy procedures: systematic review and meta-analysis of randomized controlled trials.

    Science.gov (United States)

    Wang, Man Cai; Zhang, Ling Yi; Zhang, Yu Long; Zhang, Ya Wu; Xu, Xiao Dong; Zhang, You Cheng

    2014-10-01

    Endoscopies are common clinical examinations that are somewhat painful and even cause fear and anxiety for patients. We performed this systematic review and meta-analysis of randomized controlled trials to determine the effect of music on patients undergoing various endoscopic procedures. We searched the Cochrane Library, Issue 6, 2013, PubMed, and EMBASE databases up to July 2013. Randomized controlled trials comparing endoscopies, with and without the use of music, were included. Two authors independently abstracted data and assessed risk of bias. Subgroup analyses were performed to examine the impact of music on different types of endoscopic procedures. Twenty-one randomized controlled trials involving 2,134 patients were included. The overall effect of music on patients undergoing a variety of endoscopic procedures significantly improved pain score (weighted mean difference [WMD] = -1.53, 95% confidence interval [CI] [-2.53, -0.53]), anxiety (WMD = -6.04, 95% CI [-9.61, -2.48]), heart rate (P = 0.01), arterial pressure (P music group, compared with the control group. Furthermore, music had little effect for patients undergoing colposcopy and bronchoscopy in the subanalysis. Our meta-analysis suggested that music may offer benefits for patients undergoing endoscopy, except in colposcopy and bronchoscopy. Wiley Periodicals, Inc.

  2. Comparative Analysis of Selected Motor Fitness Profile of Football Referees in Cross River and AKWA IBOM States, Nigeria

    Science.gov (United States)

    Ogabor, J. O.; Sanusi, M.; Saulawa, A. I.

    2015-01-01

    The purpose of this study was to compare selected motor fitness profile of football referees in Cross River and Akwa Ibom States. Motor fitness profiles compared were running speed and agility of the referees. Standardized equipment and procedures were employed in the tests. To achieve the objectives of the study, two research hypotheses were…

  3. Depth of anaesthesia monitoring during procedural sedation and analgesia: A systematic review and meta-analysis.

    Science.gov (United States)

    Conway, Aaron; Sutherland, Joanna

    2016-11-01

    Processed electroencephalogram-based depth of anaesthesia monitoring devices provide an additional method to monitor level of consciousness during procedural sedation and analgesia. The objective of this systematic review was to determine whether using a depth of anaesthesia monitoring device improves the safety and efficacy of sedation. Systematic review and meta-analysis. Electronic databases (CENTRAL; Medline; CINAHL) were searched up to May 2015. Randomised controlled trials that compared use of a depth of anaesthesia monitoring device to a control group who received standard monitoring during procedural sedation and analgesia were included. Study selection, data extraction and risk of bias assessment (Cochrane risk of bias tool) were performed by two reviewers. Safety outcomes were hypoxaemia, hypotension and adverse events. Efficacy outcomes were amount of sedation used, duration of sedation recovery and rate of incomplete procedures. A total of 16 trials (2138 participants) were included. Evidence ratings were downgraded to either low or moderate quality due to study limitations and imprecision. Meta-analysis of 8 trials (766 participants) found no difference in hypoxaemia (RR 0.87; 95% CI=0.67-1.12). No statistically significant difference in hypotension was observed in meta-analysis of 8 trials (RR 0.96; 95% CI=0.54-1.7; 942 participants). Mean dose of propofol was 51mg lower for participants randomised to depth of anaesthesia monitoring (95% CI=-88.7 to -13.3mg) in meta-analysis of results from four trials conducted with 434 participants who underwent interventional endoscopy procedures with propofol infusions to maintain sedation. The difference in recovery time between depth of anaesthesia and standard monitoring groups was not clinically significant (standardised mean difference -0.41; 95% CI=-0.8 to -0.02; I2=86%; 8 trials; 809 participants). Depth of anaesthesia monitoring did impact sedation titration during interventional procedures with propofol

  4. Partial lateral facetectomy plus Insall's procedure for the treatment of isolated patellofemoral osteoarthritis: survival analysis.

    Science.gov (United States)

    Montserrat, Ferran; Alentorn-Geli, Eduard; León, Vicente; Ginés-Cespedosa, Alberto; Rigol, Pau

    2014-01-01

    The purpose of this study was to report the survival analysis of partial lateral facetectomy and Insall's procedure in patients with isolated patellofemoral osteoarthritis, and to assess the risk and protective factors for failure of this procedure. From 1992 to 2004, all subjects with isolated patellofemoral osteoarthritis who met the inclusion criteria and underwent this procedure were enrolled. Risk and protective factors for failure (failure considered as the need for total knee arthroplasty) were assessed by comparing obtained baseline data between failed and non-failed cases. Eighty-seven cases (mean (SD) age 61.8 (7.7) years, mean (SD) follow-up 9.6 (3.2) years) were included. Twenty-three failed cases were found. Mean (SD) survival time was 13.6 (0.5) years. At 13 years (last failure case), the cumulative survival was 59.3 %. Baseline medial tibiofemoral pain, genu flexum, and worst grade of tibiofemoral osteoarthritis were significant risk factors for failure (p < 0.0001, p = 0.02, p < 0.0001, respectively). In contrast, higher anatomical (p = 0.02) and total (p = 0.03) knee society score (KSS) scores, absence of knee effusion (p = 0.03), higher value of the Caton-Deschamps index (p = 0.03), and lateral position of the patella (p = 0.01) were all protective factors against failure. The treatment for isolated patellofemoral osteoarthritis through partial lateral facetectomy and Insall's procedure demonstrated good long-term survival. The presence of preoperative medial tibiofemoral pain, genu flexum, and incipient tibiofemoral osteoarthritis increased the risk of failure of this procedure. In contrast, higher anatomical and total KSS scores, absence of knee effusion, higher value of the Caton-Deschamps index, and lateral position of the patella were found to protect against failure.

  5. Secondary Analysis of Audio Data. Technical Procedures for Virtual Anonymization and Pseudonymization

    Directory of Open Access Journals (Sweden)

    Henning Pätzold

    2005-01-01

    Full Text Available Qualitative material presented as audio data requires a greater degree of protecting of anonymity than for example textual data. Apart from the verbal content, it carries paraverbal aspects including voice characteristics, thus making it easier to identify the speaker. This complicates secondary analysis or reanalysis conducted by researchers who were not involved in the data collection. Difficulties increase if the chances are high that the researcher and the interviewee come in contact for example through a meeting. This paper describes the technical procedures that are used to modify the sound of the audio source in a way that it reduces the possibility of recognition (i.e. similar to that of a carefully written transcript. A discussion of the technical possibilities of this procedure along with an exploration of the boundaries of anonymization is presented. URN: urn:nbn:de:0114-fqs0501249

  6. Simplified Procedure For The Free Vibration Analysis Of Rectangular Plate Structures With Holes And Stiffeners

    Directory of Open Access Journals (Sweden)

    Cho Dae Seung

    2015-04-01

    Full Text Available Thin and thick plates, plates with holes, stiffened panels and stiffened panels with holes are primary structural members in almost all fields of engineering: civil, mechanical, aerospace, naval, ocean etc. In this paper, a simple and efficient procedure for the free vibration analysis of such elements is presented. It is based on the assumed mode method and can handle different plate thickness, various shapes and sizes of holes, different framing sizes and types as well as different combinations of boundary conditions. Natural frequencies and modes are determined by solving an eigenvalue problem of a multi-degree-of-freedom system matrix equation derived by using Lagrange’s equations. Mindlin theory is applied for a plate and Timoshenko beam theory for stiffeners. The applicability of the method in the design procedure is illustrated with several numerical examples obtained by the in-house developed code VAPS. Very good agreement with standard commercial finite element software is achieved.

  7. Development of a thermal and structural analysis procedure for cooled radial turbines

    Science.gov (United States)

    Kumar, Ganesh N.; Deanna, Russell G.

    1988-01-01

    A procedure for computing the rotor temperature and stress distributions in a cooled radial turbine is considered. Existing codes for modeling the external mainstream flow and the internal cooling flow are used to compute boundary conditions for the heat transfer and stress analyses. An inviscid, quasi three-dimensional code computes the external free stream velocity. The external velocity is then used in a boundary layer analysis to compute the external heat transfer coefficients. Coolant temperatures are computed by a viscous one-dimensional internal flow code for the momentum and energy equation. These boundary conditions are input to a three-dimensional heat conduction code for calculation of rotor temperatures. The rotor stress distribution may be determined for the given thermal, pressure and centrifugal loading. The procedure is applied to a cooled radial turbine which will be tested at the NASA Lewis Research Center. Representative results from this case are included.

  8. Comparison of the Nuss versus Ravitch procedure for pectus excavatum repair: an updated meta-analysis.

    Science.gov (United States)

    Mao, Yong Zhong; Tang, ShaoTao; Li, Shuai

    2017-10-01

    To evaluate surgical outcomes of Nuss versus Ravitch repair of pectus excavatum via a systematic review and meta-analysis. Medline, PubMed, Cochrane, EMBASE, and Google Scholar databases were searched up to September 5, 2016 using the following search terms: pectus excavatum, funnel chest, Nuss; Ravitch, minimally invasive, and open surgery. Randomized controlled trials, two-arm prospective, and two-arm retrospective studies were eligible for inclusion. Nineteen studies were included with a total of 1731 patients: 989 treated with Nuss and 742 treated with Ravitch. The overall analysis revealed that patients in the Nuss group had significantly shorter operation time (pooled SMD=-2.83, 95% CI=-3.76 to -1.90, p<0.001) and less blood loss (pooled SMD=-1.68, 95% CI=-2.28 to -1.09, P<0.001) than the Ravitch group. However, the length of hospital stay was similar between groups (pooled SMD=-0.55, 95% CI=-1.44 to 0.35, p=0.230). These findings were similar in the subgroup analysis for randomized and non-randomized controlled studies. Complications were not assessed due to inconsistent reporting across the included studies. Our meta-analysis demonstrate that the Nuss procedure has a shorter operative time and less operative blood loss than the Ravitch procedure while the postoperative length of stay was similar. Level III. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Procedure for conducting probabilistic safety assessment: level 1 full power internal event analysis

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Won Dae; Lee, Y. H.; Hwang, M. J. [and others

    2003-07-01

    This report provides guidance on conducting a Level I PSA for internal events in NPPs, which is based on the method and procedure that was used in the PSA for the design of Korea Standard Nuclear Plants (KSNPs). Level I PSA is to delineate the accident sequences leading to core damage and to estimate their frequencies. It has been directly used for assessing and modifying the system safety and reliability as a key and base part of PSA. Also, Level I PSA provides insights into design weakness and into ways of preventing core damage, which in most cases is the precursor to accidents leading to major accidents. So Level I PSA has been used as the essential technical bases for risk-informed application in NPPs. The report consists six major procedural steps for Level I PSA; familiarization of plant, initiating event analysis, event tree analysis, system fault tree analysis, reliability data analysis, and accident sequence quantification. The report is intended to assist technical persons performing Level I PSA for NPPs. A particular aim is to promote a standardized framework, terminology and form of documentation for PSAs. On the other hand, this report would be useful for the managers or regulatory persons related to risk-informed regulation, and also for conducting PSA for other industries.

  10. IC Treatment: Surgical Procedures

    Science.gov (United States)

    ... Profile Home Diagnosis & Treatment IC Treatments Surgical Procedures Surgical Procedures For people with interstitial cystitis (IC), the ... latest stories, news and events. Please leave this field empty Interstitial Cystitis Association 7918 Jones Branch Drive, ...

  11. Metabolic profiling of body fluids and multivariate data analysis.

    Science.gov (United States)

    Trezzi, Jean-Pierre; Jäger, Christian; Galozzi, Sara; Barkovits, Katalin; Marcus, Katrin; Mollenhauer, Brit; Hiller, Karsten

    2017-01-01

    Metabolome analyses of body fluids are challenging due pre-analytical variations, such as pre-processing delay and temperature, and constant dynamical changes of biochemical processes within the samples. Therefore, proper sample handling starting from the time of collection up to the analysis is crucial to obtain high quality samples and reproducible results. A metabolomics analysis is divided into 4 main steps: 1) Sample collection, 2) Metabolite extraction, 3) Data acquisition and 4) Data analysis. Here, we describe a protocol for gas chromatography coupled to mass spectrometry (GC-MS) based metabolic analysis for biological matrices, especially body fluids. This protocol can be applied on blood serum/plasma, saliva and cerebrospinal fluid (CSF) samples of humans and other vertebrates. It covers sample collection, sample pre-processing, metabolite extraction, GC-MS measurement and guidelines for the subsequent data analysis. Advantages of this protocol include: •Robust and reproducible metabolomics results, taking into account pre-analytical variations that may occur during the sampling process•Small sample volume required•Rapid and cost-effective processing of biological samples•Logistic regression based determination of biomarker signatures for in-depth data analysis.

  12. Profiling safety of intravitreal injections for retinoblastoma using an anti-reflux procedure and sterilisation of the needle track.

    Science.gov (United States)

    Munier, Francis L; Soliman, Sameh; Moulin, Alexandre P; Gaillard, Marie-Claire; Balmer, Aubin; Beck-Popovic, Maja

    2012-08-01

    The preservation of globe integrity has always been a major concern during the treatment of retinoblastoma for fear of extraocular or metastatic spread. Intravitreal chemotherapy has been attempted as a desperate salvage therapy only for eyes with refractory retinoblastoma. Published data on the safety and efficacy of this route are, however, limited. A modified technique of intravitreal injection in eyes with retinoblastoma is described. All children with retinoblastoma who received one or more intravitreal injections using this technique were retrospectively reviewed concerning ocular complications of the injection procedure as well as clinical or histopathological evidence of tumour spread. 30 eyes of 30 children with retinoblastoma received a total of 135 intravitreal injections, with a median follw-up duration of 13.5 months. No extraocular spread was seen on clinical follow-up in any patients and there was no tumour contamination of the retrieved entry sites histopathologically analysed among the five enucleated eyes. No significant ocular side effects were observed except transient localised vitreous haemorrhage (3/135). This technique is potentially safe and effective at a low cost and may play a promising role, especially in the treatment of recurrent and/or resistant vitreous disease in retinoblastoma, as an alternative to enucleation and/or external beam radiotherapy. However, this treatment should not replace the primary standard of care of retinoblastoma and should not be considered in group E eyes. Its application should be approved by an ophthalmological-oncological team and it should be performed by an experienced eye surgeon in a tertiary referral centre after careful selection of a tumour-free injection site.

  13. Steroid hormones profile during an ovarian synchronization procedure in different age categories of red deer hinds (Cervus elaphus L.

    Directory of Open Access Journals (Sweden)

    Šperanda Marcela

    2012-01-01

    Full Text Available The objective of the present study was to compare estradiol/progesterone ratios of different age categories of red deer hinds and use it as a predictor of estrus synchronization success and consequently conception rate. To accomplish this we used 38 red deer hinds to establish serum progesterone and estradiol levels in young (21 animals, mature (10 animals and old (7 animals hinds during the estrus synchronization procedure (transvaginal/cervical AI. The following estrus synchronization was used: at the start of the experiment each hind received a controlled intravaginal drug-releasing device (CIDR, Pharmacia&Upjohn, New Zealand containing 0.3 g of progesterone. The device was removed on day 11, simultaneously with an application of 250 IU of Pregnant Mare Serum Gonadotropin (PMSG, Folligon® Intervet International, Boxmeer, Holland. Transvaginal/ cervical AI (artificial insemination was performed 48 hours after CIDR withdrawal (day 13. Blood samples were obtained from the jugular vein using a Venoject® vacutainer without an anticoagulant for hormonal tests on the same experimental day (0, 11th and 13th day. A statistically (p<0.01 higher progesterone level was found in young hinds on the 11th day after controlled intravaginal drug-releasing device insertion. A significantly higher (p<0.01 estrogen level was observed in the young in regard to mature and old hinds on the expected day of estrus (13th day. Estradiol/progesterone ratios showed a statistically significant difference (p<0.01 on insemination day (13th day between old and young hinds (98.67 : 46.59 and between old and mature hinds (98.67 : 51.79. Out of a total of 38 hinds only 9 had their offspring, 6 of the young and 3 of the mature hinds.

  14. Salvia broussonetii Benth.: aroma profile and micromorphological analysis.

    Science.gov (United States)

    Najar, Basma; Pistelli, Luisa; Cervelli, Claudio; Fico, Gelsomina; Giuliani, Claudia

    2017-10-26

    The volatile profiles (VOC) and the essential oil (EO) composition from the aerial parts of Salvia broussonetii were analysed. Sesquiterpene hydrocarbons dominate the VOCs from leaves (95.7%) and flowers (67.6%), followed by monoterpene hydrocarbons (2.6 and 29.7%, respectively). The main common compounds are germacrene D, β-bourbonene, α-pinene, α-copaene and α-gurjunene, even if with divergent relative abundances. In the leaf EOs the sesquiterpenes prevail, even if not overwhelmingly (about 50.0%), followed by monoterpenes (23.0-35.0%) and by minor fractions of diterpene hydrocarbons and non-terpene derivates. The most abundant common compounds across the two sampling periods are α-pinene, β-pinene, isobornyl acetate, α-gurjenene, germacrene D and bifloratriene. A morphological characterisation of the trichomes responsible for the productivity in terpenes was also performed. Four different morphotypes were observed on the above ground organs of S. brussonetii: peltates and capitates of type II and III resulted the only producers of volatile substances.

  15. Confirmatory factor analysis of the Oral Health Impact Profile.

    Science.gov (United States)

    John, M T; Feuerstahler, L; Waller, N; Baba, K; Larsson, P; Celebić, A; Kende, D; Rener-Sitar, K; Reissmann, D R

    2014-09-01

    Previous exploratory analyses suggest that the Oral Health Impact Profile (OHIP) consists of four correlated dimensions and that individual differences in OHIP total scores reflect an underlying higher-order factor. The aim of this report is to corroborate these findings in the Dimensions of Oral Health-Related Quality of Life (DOQ) Project, an international study of general population subjects and prosthodontic patients. Using the project's Validation Sample (n = 5022), we conducted confirmatory factor analyses in a sample of 4993 subjects with sufficiently complete data. In particular, we compared the psychometric performance of three models: a unidimensional model, a four-factor model and a bifactor model that included one general factor and four group factors. Using model-fit criteria and factor interpretability as guides, the four-factor model was deemed best in terms of strong item loadings, model fit (RMSEA = 0·05, CFI = 0·99) and interpretability. These results corroborate our previous findings that four highly correlated factors - which we have named Oral Function, Oro-facial Pain, Oro-facial Appearance and Psychosocial Impact - can be reliably extracted from the OHIP item pool. However, the good fit of the unidimensional model and the high interfactor correlations in the four-factor solution suggest that OHRQoL can also be sufficiently described with one score. © 2014 John Wiley & Sons Ltd.

  16. THE STRUCTURAL ANALYSIS OF STEEL SILOS WITH CYLINDRICAL-WALL BEARING AND PROFILE-STEEL BEARING

    Directory of Open Access Journals (Sweden)

    Zhengjun Tang

    2015-04-01

    Full Text Available The silos are widely used in bulk material in many fields such as agriculture, mining, chemical, electric power storage, etc. Thin metal cylindrical silo shells are vulnerable to buckling failure caused by the compressive wall friction force. In this paper, the structural analysis of two types of steel silo with cylindrical-wall bearing and profile-steel bearing is implemented by Abaqus finite element analysis. The results indicate that under the same loading conditions, steel silos with profile-steel bearing and cylindrical-Wall bearing have similar values in Mises stress, but the steel silo with profile-steel bearing has a smaller radial displacement and a better capability of buckling resistance. Meanwhile, the total steel volumes reduced 8.0% comparing to the steel silo with cylindrical-wall bearing. Therefore, steel soil with profile-steel bearing not only has a less steel volumes but also a good stability.

  17. Cluster analysis of residential heat load profiles and the role of technical and household characteristics

    DEFF Research Database (Denmark)

    Carmo, Carolina; Christensen, Toke Haunstrup

    2016-01-01

    of the temporality of the energy demand is needed. This paper contributes to this by focusing on the daily load profiles of energy demand for heating of Danish dwellings with heat pumps. Based on hourly recordings from 139 dwellings and employing cluster and regression analysis, the paper explores patterns...... (typologies) in daily heating load profiles and how these relate to socio-economic and technical characteristics of the included households. The study shows that the load profiles vary according to the external load conditions. Two main clusters were identified for both weekdays and weekends and across load...

  18. Spatial mapping of correlation profile in Brillouin optical correlation domain analysis

    Science.gov (United States)

    Somepalli, Bhargav; Venkitesh, Deepa; Srinivasan, Balaji

    2017-04-01

    We report an approach to spatially map the correlation profile along the sensing fiber in Brillouin optical correlation domain analysis by pulsing the pump radiation. Simulations are carried out to demonstrate the influence of frequency modulation parameters of a narrow linewidth source on the width of the correlation profile and its peak position. The simulation results are validated through controlled experiments. The correlation profile is mapped over 1 km long fiber with spatial resolution of 1 m, limited only by the finite lifetime of acoustic phonons in the silica fiber.

  19. A simple analysis of the stable field profile in the supercritical TEA

    DEFF Research Database (Denmark)

    Jeppesen, Palle; Jeppsson, B.

    1973-01-01

    An analytical investigation supported by numerical calculations has been performed of the stable field profile in a supercritical diffusion-stabilized n-GaAs transferred electron amplifier (TEA) with ohmic contacts. In the numerical analysis, the field profile is determined by solving the steady......}{2}} (n_{0}L)^{frac{1}{2}}. Those results contribute to the understanding of the highn_{0}L-product switch and the stability of the supercritical TEA....

  20. A Latent Profile Analysis of Latino Parenting: The Infusion of Cultural Values on Family Conflict

    OpenAIRE

    Ayón, Cecilia; Williams, Lela Rankin; Marsiglia, Flavio F.; Ayers, Stephanie; Kiehne, Elizabeth

    2015-01-01

    The purpose of the present study was to (a) examine how acculturation and social support inform Latinos’ parenting behaviors, controlling for gender and education; (b) describe parenting styles among Latino immigrants while accounting for cultural elements; and (c) test how these parenting styles are associated with family conflict. A 3 step latent profile analysis with the sample (N = 489) revealed best fit with a 4 profile model (n = 410) of parenting: family parenting (n = 268, 65%), child...

  1. Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples

    DEFF Research Database (Denmark)

    Belstrøm, Daniel; Holmstrup, Palle; Jensen, Allan Bardow

    2016-01-01

    BACKGROUND AND OBJECTIVE: The microbial profiles of stimulated saliva samples have been shown to differentiate between patients with periodontitis, patients with dental caries, and orally healthy individuals. Saliva was stimulated to allow for easy and rapid collection; however, microbial...... orally and systemically healthy, non-smoking participants. Salivary bacterial profiles were analyzed by means of the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS), and statistical analysis was performed using Mann-Whitney test with Benjamini-Hochberg's correction...

  2. Latent Profile and Cluster Analysis of Infant Temperament: Comparisons across Person-Centered Approaches

    Science.gov (United States)

    Gartstein, Maria A.; Prokasky, Amanda; Bell, Martha Ann; Calkins, Susan; Bridgett, David J.; Braungart-Rieker, Julia; Leerkes, Esther; Cheatham, Carol L.; Eiden, Rina D.; Mize, Krystal D.; Jones, Nancy Aaron; Mireault, Gina; Seamon, Erich

    2017-01-01

    There is renewed interest in person-centered approaches to understanding the structure of temperament. However, questions concerning temperament types are not frequently framed in a developmental context, especially during infancy. In addition, the most common person-centered techniques, cluster analysis (CA) and latent profile analysis (LPA),…

  3. The Social Profile of Students in Basic General Education in Ecuador: A Data Analysis

    Science.gov (United States)

    Buri, Olga Elizabeth Minchala; Stefos, Efstathios

    2017-01-01

    The objective of this study is to examine the social profile of students who are enrolled in Basic General Education in Ecuador. Both a descriptive and multidimensional statistical analysis was carried out based on the data provided by the National Survey of Employment, Unemployment and Underemployment in 2015. The descriptive analysis shows the…

  4. General Staining and Segmentation Procedures for High Content Imaging and Analysis.

    Science.gov (United States)

    Chambers, Kevin M; Mandavilli, Bhaskar S; Dolman, Nick J; Janes, Michael S

    2018-01-01

    Automated quantitative fluorescence microscopy, also known as high content imaging (HCI), is a rapidly growing analytical approach in cell biology. Because automated image analysis relies heavily on robust demarcation of cells and subcellular regions, reliable methods for labeling cells is a critical component of the HCI workflow. Labeling of cells for image segmentation is typically performed with fluorescent probes that bind DNA for nuclear-based cell demarcation or with those which react with proteins for image analysis based on whole cell staining. These reagents, along with instrument and software settings, play an important role in the successful segmentation of cells in a population for automated and quantitative image analysis. In this chapter, we describe standard procedures for labeling and image segmentation in both live and fixed cell samples. The chapter will also provide troubleshooting guidelines for some of the common problems associated with these aspects of HCI.

  5. Acute Myeloid Leukemia: analysis of epidemiological profile and survival rate.

    Science.gov (United States)

    de Lima, Mariana Cardoso; da Silva, Denise Bousfield; Freund, Ana Paula Ferreira; Dacoregio, Juliana Shmitz; Costa, Tatiana El Jaick Bonifácio; Costa, Imaruí; Faraco, Daniel; Silva, Maurício Laerte

    2016-01-01

    To describe the epidemiological profile and the survival rate of patients with acute myeloid leukemia (AML) in a state reference pediatric hospital. Clinical-epidemiological, observational, retrospective, descriptive study. The study included new cases of patients with AML, diagnosed between 2004 and 2012, younger than 15 years. Of the 51 patients studied, 84% were white; 45% were females and 55%, males. Regarding age, 8% were younger than 1 year, 47% were aged between 1 and 10 years, and 45% were older than 10 years. The main signs/symptoms were fever (41.1%), asthenia/lack of appetite (35.2%), and hemorrhagic manifestations (27.4%). The most affected extra-medullary site was the central nervous system (14%). In 47% of patients, the white blood cell (WBC) count was below 10,000/mm(3) at diagnosis. The minimal residual disease (MRD) was less than 0.1%, on the 15th day of treatment in 16% of the sample. Medullary relapse occurred in 14% of cases. When comparing the bone marrow MRD with the vital status, it was observed that 71.42% of the patients with type M3 AML were alive, as were 54.05% of those with non-M3 AML. The death rate was 43% and the main proximate cause was septic shock (63.6%). In this study, the majority of patients were male, white, and older than 1 year. Most patients with WBC count <10,000/mm(3) at diagnosis lived. Overall survival was higher in patients with MRD <0.1%. The prognosis was better in patients with AML-M3. Copyright © 2016 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.

  6. Processes and Procedures for Application of CFD to Nuclear Reactor Safety Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Richard W. Johnson; Richard R. Schultz; Patrick J. Roache; Ismail B. Celik; William D. Pointer; Yassin A. Hassan

    2006-09-01

    Traditionally, nuclear reactor safety analysis has been performed using systems analysis codes such as RELAP5, which was developed at the INL. However, goals established by the Generation IV program, especially the desire to increase efficiency, has lead to an increase in operating temperatures for the reactors. This increase pushes reactor materials to operate towards their upper temperature limits relative to structural integrity. Because there will be some finite variation of the power density in the reactor core, there will be a potential for local hot spots to occur in the reactor vessel. Hence, it has become apparent that detailed analysis will be required to ensure that local ‘hot spots’ do not exceed safety limits. It is generally accepted that computational fluid dynamics (CFD) codes are intrinsically capable of simulating fluid dynamics and heat transport locally because they are based on ‘first principles.’ Indeed, CFD analysis has reached a fairly mature level of development, including the commercial level. However, CFD experts are aware that even though commercial codes are capable of simulating local fluid and thermal physics, great care must be taken in their application to avoid errors caused by such things as inappropriate grid meshing, low-order discretization schemes, lack of iterative convergence and inaccurate time-stepping. Just as important is the choice of a turbulence model for turbulent flow simulation. Turbulence models model the effects of turbulent transport of mass, momentum and energy, but are not necessarily applicable for wide ranges of flow types. Therefore, there is a well-recognized need to establish practices and procedures for the proper application of CFD to simulate flow physics accurately and establish the level of uncertainty of such computations. The present document represents contributions of CFD experts on what the basic practices, procedures and guidelines should be to aid CFD analysts to obtain accurate

  7. Meta-analysis of the technical performance of an imaging procedure: guidelines and statistical methodology.

    Science.gov (United States)

    Huang, Erich P; Wang, Xiao-Feng; Choudhury, Kingshuk Roy; McShane, Lisa M; Gönen, Mithat; Ye, Jingjing; Buckler, Andrew J; Kinahan, Paul E; Reeves, Anthony P; Jackson, Edward F; Guimaraes, Alexander R; Zahlmann, Gudrun

    2015-02-01

    Medical imaging serves many roles in patient care and the drug approval process, including assessing treatment response and guiding treatment decisions. These roles often involve a quantitative imaging biomarker, an objectively measured characteristic of the underlying anatomic structure or biochemical process derived from medical images. Before a quantitative imaging biomarker is accepted for use in such roles, the imaging procedure to acquire it must undergo evaluation of its technical performance, which entails assessment of performance metrics such as repeatability and reproducibility of the quantitative imaging biomarker. Ideally, this evaluation will involve quantitative summaries of results from multiple studies to overcome limitations due to the typically small sample sizes of technical performance studies and/or to include a broader range of clinical settings and patient populations. This paper is a review of meta-analysis procedures for such an evaluation, including identification of suitable studies, statistical methodology to evaluate and summarize the performance metrics, and complete and transparent reporting of the results. This review addresses challenges typical of meta-analyses of technical performance, particularly small study sizes, which often causes violations of assumptions underlying standard meta-analysis techniques. Alternative approaches to address these difficulties are also presented; simulation studies indicate that they outperform standard techniques when some studies are small. The meta-analysis procedures presented are also applied to actual [18F]-fluorodeoxyglucose positron emission tomography (FDG-PET) test-retest repeatability data for illustrative purposes. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  8. Primary Angioplasty in Patients Older Than 75 Years. Profile of Patients and Procedures, Outcomes, and Predictors of Prognosis in the ESTROFA IM+75 Registry.

    Science.gov (United States)

    de la Torre Hernández, José M; Brugaletta, Salvatore; Gómez Hospital, Joan A; Baz, José A; Pérez de Prado, Armando; López Palop, Ramón; Cid, Belén; García Camarero, Tamara; Diego, Alejandro; Gimeno de Carlos, Federico; Fernández Díaz, José A; Sanchis, Juan; Alfonso, Fernando; Blanco, Roberto; Botas, Javier; Navarro Cuartero, Javier; Moreu, José; Bosa, Francisco; Vegas Valle, José M; Elízaga, Jaime; Arrebola, Antonio L; Ruiz Arroyo, José R; Hernández-Hernández, Felipe; Salvatella, Neus; Monteagudo, Marta; Gómez Jaume, Alfredo; Carrillo, Xavier; Martín Reyes, Roberto; Lozano, Fernando; Rumoroso, José R; Andraka, Leire; Domínguez, Antonio J

    2017-02-01

    The proportion of elderly patients undergoing primary angioplasty is growing. The present study describes the clinical profile, procedural characteristics, outcomes, and predictors of outcome. A 31-center registry of consecutive patients older than 75 years treated with primary angioplasty. Clinical and procedural data were collected, and the patients underwent clinical follow-up. The study included 3576 patients (39.3% women, 48.5% with renal failure, 11.5% in Killip III or IV, and 29.8% with>6hours of chest pain). Multivessel disease was present in 55.4% and nonculprit lesions were additionally treated in 24.8%. Radial access was used in 56.4%, bivalirudin in 11.8%, thromboaspiration in 55.9%, and drug-eluting stents in 26.6%. The 1-month and 2-year incidences of cardiovascular death were 10.1% and 14.7%, respectively. The 2-year rates of definite or probable thrombosis, repeat revascularization, and BARC bleeding>2 were 3.1%, 2.3%, and 4.2%, respectively. Predictive factors were diabetes mellitus, renal failure, atrial fibrillation, delay to reperfusion>6hours, ejection fraction<45%, Killip class III-IV, radial access, bivalirudin, drug-eluting stents, final TIMI flow of III, and incomplete revascularization at discharge. Notable registry findings include frequently delayed presentation and a high prevalence of adverse factors such as renal failure and multivessel disease. Positive procedure-related predictors include shorter delay, use of radial access, bivalirudin, drug-eluting stents, and complete revascularization before discharge. Copyright © 2016 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.

  9. Energy Consumption Analysis Procedure for Robotic Applications in different task motion

    Science.gov (United States)

    Ahmed, Iman; Aris, Ishak b.; Hamiruce Marhaban, Mohammad; Juraiza Ishak, Asnor

    2015-11-01

    This work proposes energy analysis method for humanoid robot, seen from simple motion task to complex one in energy chain. The research developed a procedure suitable for analysis, saving and modelling of energy consumption not only in this type of robot but also in most robots that based on electrical power as an energy source. This method has validated by an accurate integration using Matlab software for the power consumption curve to calculate the energy of individual and multiple servo motors. Therefore, this study can be considered as a procedure for energy analysis by utilizing the laboratory instruments capabilities to measure the energy parameters. We performed a various task motions with different angular speed to find out the speed limits in terms of robot stability and control strategy. A battery capacity investigation have been searched for several types of batteries to extract the power modelling equation and energy density parameter for each battery type, Matlab software have been built to design the algorithm and to evaluate experimental amount of the energy which is represented by area under the curve of the power curves. This will provide a robust estimation for the required energy in different task motions to be considered in energy saving (i.e., motion planning and real time scheduling).

  10. A factor-adjusted multiple testing procedure for ERP data analysis.

    Science.gov (United States)

    Causeur, David; Chu, Mei-Chen; Hsieh, Shulan; Sheu, Ching-Fan

    2012-09-01

    Event-related potentials (ERPs) are now widely collected in psychological research to determine the time courses of mental events. When event-related potentials from treatment conditions are compared, often there is no a priori information on when or how long the differences should occur. Testing simultaneously for differences over the entire set of time points creates a serious multiple comparison problem in which the probability of false positive errors must be controlled, while maintaining reasonable power for correct detection. In this work, we extend the factor-adjusted multiple testing procedure developed by Friguet, Kloareg, and Causeur (Journal of the American Statistical Association, 104, 1406-1415, 2009) to manage the multiplicity problem in ERP data analysis and compare its performance with that of the Benjamini and Hochberg (Journal of the Royal Statistical Society B, 57, 289-300, 1995) false discovery rate procedure, using simulations. The proposed procedure outperformed the latter in detecting more truly significant time points, in addition to reducing the variability of the false discovery rate, suggesting that corrections for mass multiple testings of ERPs can be much improved by modeling the strong local temporal dependencies.

  11. The interval testing procedure: A general framework for inference in functional data analysis.

    Science.gov (United States)

    Pini, Alessia; Vantini, Simone

    2016-09-01

    We introduce in this work the Interval Testing Procedure (ITP), a novel inferential technique for functional data. The procedure can be used to test different functional hypotheses, e.g., distributional equality between two or more functional populations, equality of mean function of a functional population to a reference. ITP involves three steps: (i) the representation of data on a (possibly high-dimensional) functional basis; (ii) the test of each possible set of consecutive basis coefficients; (iii) the computation of the adjusted p-values associated to each basis component, by means of a new strategy here proposed. We define a new type of error control, the interval-wise control of the family wise error rate, particularly suited for functional data. We show that ITP is provided with such a control. A simulation study comparing ITP with other testing procedures is reported. ITP is then applied to the analysis of hemodynamical features involved with cerebral aneurysm pathology. ITP is implemented in the fdatest R package. © 2016, The International Biometric Society.

  12. Procedure for the record, calculation and analysis of costs at the Post Company of Cuba.

    Directory of Open Access Journals (Sweden)

    María Luisa Lara Zayas

    2012-12-01

    Full Text Available The Cuban Company is immersed in important changes, which lead to a new economic model that requires to increase the productivity of work and to enlarge the economic efficiency by means of rational use of material resources, financial and humans. In the present work it is proposed a procedure based on the application of cost techniques, for the record, calculation and costs analysis of activities in the Post Company of Cuba in Sancti Spiritus with the objective to obtain a major efficiency from the rational use of resources.

  13. Pathway analysis of kidney cancer using proteomics and metabolic profiling

    Directory of Open Access Journals (Sweden)

    Fiehn Oliver

    2006-11-01

    Full Text Available Abstract Background Renal cell carcinoma (RCC is the sixth leading cause of cancer death and is responsible for 11,000 deaths per year in the US. Approximately one-third of patients present with disease which is already metastatic and for which there is currently no adequate treatment, and no biofluid screening tests exist for RCC. In this study, we have undertaken a comprehensive proteomic analysis and subsequently a pathway and network approach to identify biological processes involved in clear cell RCC (ccRCC. We have used these data to investigate urinary markers of RCC which could be applied to high-risk patients, or to those being followed for recurrence, for early diagnosis and treatment, thereby substantially reducing mortality of this disease. Results Using 2-dimensional electrophoresis and mass spectrometric analysis, we identified 31 proteins which were differentially expressed with a high degree of significance in ccRCC as compared to adjacent non-malignant tissue, and we confirmed some of these by immunoblotting, immunohistochemistry, and comparison to published transcriptomic data. When evaluated by several pathway and biological process analysis programs, these proteins are demonstrated to be involved with a high degree of confidence (p values Conclusion Extensive pathway and network analysis allowed for the discovery of highly significant pathways from a set of clear cell RCC samples. Knowledge of activation of these processes will lead to novel assays identifying their proteomic and/or metabolomic signatures in biofluids of patient at high risk for this disease; we provide pilot data for such a urinary bioassay. Furthermore, we demonstrate how the knowledge of networks, processes, and pathways altered in kidney cancer may be used to influence the choice of optimal therapy.

  14. A limited assessment of the ASEP human reliability analysis procedure using simulator examination results

    Energy Technology Data Exchange (ETDEWEB)

    Gore, B.R.; Dukelow, J.S. Jr.; Mitts, T.M.; Nicholson, W.L. [Pacific Northwest Lab., Richland, WA (United States)

    1995-10-01

    This report presents a limited assessment of the conservatism of the Accident Sequence Evaluation Program (ASEP) human reliability analysis (HRA) procedure described in NUREG/CR-4772. In particular, the, ASEP post-accident, post-diagnosis, nominal HRA procedure is assessed within the context of an individual`s performance of critical tasks on the simulator portion of requalification examinations administered to nuclear power plant operators. An assessment of the degree to which operator perforn:Lance during simulator examinations is an accurate reflection of operator performance during actual accident conditions was outside the scope of work for this project; therefore, no direct inference can be made from this report about such performance. The data for this study are derived from simulator examination reports from the NRC requalification examination cycle. A total of 4071 critical tasks were identified, of which 45 had been failed. The ASEP procedure was used to estimate human error probability (HEP) values for critical tasks, and the HEP results were compared with the failure rates observed in the examinations. The ASEP procedure was applied by PNL operator license examiners who supplemented the limited information in the examination reports with expert judgment based upon their extensive simulator examination experience. ASEP analyses were performed for a sample of 162 critical tasks selected randomly from the 4071, and the results were used to characterize the entire population. ASEP analyses were also performed for all of the 45 failed critical tasks. Two tests were performed to assess the bias of the ASEP HEPs compared with the data from the requalification examinations. The first compared the average of the ASEP HEP values with the fraction of the population actually failed and it found a statistically significant factor of two bias on the average.

  15. Electrically evoked compound action potentials artefact rejection by independent component analysis: procedure automation.

    Science.gov (United States)

    Akhoun, Idrick; McKay, Colette; El-Deredy, Wael

    2015-01-15

    Independent-components-analysis (ICA) successfully separated electrically-evoked compound action potentials (ECAPs) from the stimulation artefact and noise (ECAP-ICA, Akhoun et al., 2013). This paper shows how to automate the ECAP-ICA artefact cancellation process. Raw-ECAPs without artefact rejection were consecutively recorded for each stimulation condition from at least 8 intra-cochlear electrodes. Firstly, amplifier-saturated recordings were discarded, and the data from different stimulus conditions (different current-levels) were concatenated temporally. The key aspect of the automation procedure was the sequential deductive source categorisation after ICA was applied with a restriction to 4 sources. The stereotypical aspect of the 4 sources enables their automatic classification as two artefact components, a noise and the sought ECAP based on theoretical and empirical considerations. The automatic procedure was tested using 8 cochlear implant (CI) users and one to four stimulus electrodes. The artefact and noise sources were successively identified and discarded, leaving the ECAP as the remaining source. The automated ECAP-ICA procedure successfully extracted the correct ECAPs compared to standard clinical forward masking paradigm in 22 out of 26 cases. ECAP-ICA does not require extracting the ECAP from a combination of distinct buffers as it is the case with regular methods. It is an alternative that does not have the possible bias of traditional artefact rejections such as alternate-polarity or forward-masking paradigms. The ECAP-ICA procedure bears clinical relevance, for example as the artefact rejection sub-module of automated ECAP-threshold detection techniques, which are common features of CI clinical fitting software. Copyright © 2014. Published by Elsevier B.V.

  16. Criteria and procedures for validating biomathematical models of human performance and fatigue : procedures for analysis of work schedules.

    Science.gov (United States)

    2013-01-01

    Each railroad covered by 49 CFR 228.407 must perform an analysis of the work schedules of its train employees who are engaged in commuter or intercity rail passenger transportation and identify those schedules that, if worked by such a train employee...

  17. An Excel™-VBA programme for the analysis of current velocity profiles

    Science.gov (United States)

    Le Roux, J. P.; Brodalka, M.

    2004-10-01

    VPA is an Excel spreadsheet to facilitate the analysis of current velocity profiles and its application to sediment transport studies in steady, uniform, open-channel flows. The program requires input values such as the water temperature (from which the density and dynamic viscosity are calculated), the channel depth and slope, current velocities as measured at different heights above the bed, bedform length and height, as well as the sediment density and median size. The latter can be provided as sieve diameters, fall diameters or as phi values. The velocity profiles are plotted on two graphs, one being a traditional plot of velocity versus height or distance from the bed and the other comparing the observed profile with theoretical profiles for smooth, transitional and rough boundary conditions. VBA macros are provided to clear the spreadsheet before new profiles are analysed, update the formulas, straighten out the velocity profiles, calculate the shear velocity, and save the data on a separate sheet for further analysis. The programme is applied to a new and more accurate method to determine the shear velocity, which can be used to predict the bedload discharge over plane beds and is also incorporated into a dimensionally correct suspended load transport equation combining the parameters most important in sediment transport. A dimensionally correct bedload discharge equation based upon the mean excess flow velocity is also proposed for plane beds, ripples and dunes.

  18. Treatment Strategy Profiles in Substance Use Disorder Treatment Programs: A Latent Class Analysis

    Science.gov (United States)

    Aletraris, Lydia; Paino, Maria; Roman, Paul M.

    2015-01-01

    Background Modern treatment options for substance use disorder are diverse. While studies have analyzed the adoption of individual evidence-based practices in treatment centers, little is known about the specific make-up of treatment strategy profiles in treatment centers throughout the United States. The current study used latent class analysis to profile underlying treatment strategies and to evaluate philosophical and structural supports associated with each profile. Methods Utilizing three aggregated and secondary datasets of nationally representative samples of substance use disorder treatment centers (N=775), we employed latent class analysis to determine treatment strategy profiles. Using multinomial logistic regression, we then examined organizational characteristics associated with each profile. Results We found three distinct treatment strategy profiles: Centers that primarily relied on Motivational Interviewing and Motivational Enhancement Therapy, centers that utilized psychosocial and alternative therapies, and centers that employed comprehensive treatments including pharmacotherapy. The multinomial logistic regression revealed that philosophical and structural center characteristics were associated with membership in the comprehensive class. Centers with philosophical orientations conducive to holistic care and pharmacotherapy-acceptance, resource-rich infrastructures, and an entrepreneurial reliance on insured clients were more likely to offer diverse interventions. All associations were significant at the .05 level. Principle Conclusion The findings from this study help us understand the general strategies of treatment centers. From a practical perspective, practitioners and clients should be aware of the variation in treatment center practices where they may offer or receive treatment. PMID:26105707

  19. Multimorbidity Profiles in German Centenarians: A Latent Class Analysis of Health Insurance Data.

    Science.gov (United States)

    Gellert, Paul; von Berenberg, Petra; Zahn, Thomas; Neuwirth, Julia; Kuhlmey, Adelheid; Dräger, Dagmar

    2017-10-01

    Multimorbidity in centenarians is common; although investigations of the prevalence of morbidity in centenarians are accumulating, research on profiles of co-occurrence of morbidities is still sparse. Our aim was to explore profiles of comorbidities in centenarians. Health insurance data from 1,121 centenarians comprising inpatient and outpatient diagnoses from the past 5 years (2009-2013) were analyzed using latent class analysis with adjustments for sex, age, hospitalization, and long-term care. Four distinct comorbidity profiles emerged from the data: 36% of centenarians were categorized as "age-associated"; 18% had a variety of comorbidities but were not diabetic were labeled "multimorbid without diabetes"; 9% were labeled "multimorbid with diabetes"; and 36% "low morbidity." Patterns of comorbidities describe the complexity of geriatric multimorbidity more appropriately than an approach focused on a single disease. The profiles described by this specific research may inform clinicians and health care planners for the oldest old.

  20. Sedation for pediatric radiological procedures: analysis of potential causes of sedation failure and paradoxical reactions

    Energy Technology Data Exchange (ETDEWEB)

    Karian, V.E.; Burrows, P.E.; Connor, L. [Dept. of Radiology, Children' s Hospital, Boston, MA (United States); Zurakowski, D. [Dept. of Biostatistics, Children' s Hospital, Boston, MA (United States); Mason, K.P. [Dept. of Anesthesiology, Children' s Hospital, Boston, MA (United States)

    1999-11-01

    Background. Sedation for diagnostic imaging and interventional radiologic procedures in pediatrics has greatly increased over the past decade. With appropriate patient selection and monitoring, serious adverse effects are infrequent, but failure to sedate and paradoxical reactions do occur. Objective. The purpose of this study was to determine, among patients undergoing sedation for radiologic procedures, the incidence of sedation failure and paradoxical reaction to pentobarbital and to identify potentially correctable causes. Materials and methods. Records of 1665 patients who were sedated in the radiology department from 1 November 1997 to 1 July 1998 were reviewed. Patients failing sedation or experiencing paradoxical reaction were compared with respect to sex, age group, diagnosis, scan type, time of day, NPO status, use of IV contrast and type of sedation agent using the Fisher exact test, Pearson chi-square, analysis of variance (ANOVA), the Student t-test, and logistic regression. Results. Data analysis revealed a sedation failure rate of 1 % and paradoxical reaction rate of 1.2 %. Stepwise multiple logistic regression revealed that the only significant independent multivariate predictor of failure was the need for the administration of a combination of pentobarbital, fentanyl, and midazolam IV. Conclusion. The low rate of sedation failure and paradoxical reactions to pentobarbital was near optimal and probably cannot be improved with the currently available sedatives. (orig.)

  1. Neutron activation analysis with k{sub 0}-standardisation : general formalism and procedure

    Energy Technology Data Exchange (ETDEWEB)

    Pomme, S.; Hardeman, F. [Centre de l`Etude de l`Energie Nucleaire, Mol (Belgium); Robouch, P.; Etxebarria, N.; Arana, G. [European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (Belgium)

    1997-09-01

    Instrumental neutron activation analysis (INAA) with k{sub 0}-standardisation is a powerful tool for multi-element analysis at a broad range of trace element concentrations. An overview is given of the basic principles, fundamental equations, and general procedure of this method. Different aspects of the description of the neutron activation reaction rate are discussed, applying the Hogdahl convention. A general activation-decay formula is derived and its application to INAA is demonstrated. Relevant k{sub 0}-definitions for different activation decay schemes are summarised and upgraded to cases of extremely high fluxes. The main standardisation techniques for INAA are discussed, emphasizing the k{sub 0}-standardisation. Some general aspects of the basic equipment and its calibration are discussed, such as the characterisation of the neutron field and the tuning of the spectrometry part. A method for the prediction and optimisation of the analytical performance of INAA is presented.

  2. Mindfulness and Psychological Health Outcomes: A Latent Profile Analysis among Military Personnel and College Students.

    Science.gov (United States)

    Bravo, Adrian J; Pearson, Matthew R; Kelley, Michelle L

    2018-02-01

    Previous research on trait mindfulness facets using person-centered analyses (e.g., latent profile analysis [LPA]) has identified four distinct mindfulness profiles among college students: a high mindfulness group (high on all facets of the Five-Factor Mindfulness Questionnaire [FFMQ]), a judgmentally observing group (highest on observing, but low on non-judging of inner experience and acting with awareness), a non-judgmentally aware group (high on non-judging of inner experience and acting with awareness, but very low on observing), and a low mindfulness group (low on all facets of the FFMQ). In the present study, we used LPA to identify distinct mindfulness profiles in a community based sample of U.S. military personnel (majority veterans; n = 407) and non-military college students ( n = 310) and compare these profiles on symptoms of psychological health outcomes (e.g., suicidality, PTSD, anxiety, rumination) and percentage of participants exceeding clinically significant cut-offs for depressive symptoms, substance use, and alcohol use. In the subsample of college students, we replicated previous research and found four distinct mindfulness profiles; however, in the military subsample we found three distinct mindfulness profiles (a combined low mindfulness/judgmentally observing class). In both subsamples, we found that the most adaptive profile was the "high mindfulness" profile (i.e., demonstrated the lowest scores on all psychological symptoms and the lowest probability of exceeding clinical cut-offs). Based on these findings, we purport that the comprehensive examination of an individual's mindfulness profile could help clinicians tailor interventions/treatments that capitalize on individual's specific strengths and work to address their specific deficits.

  3. Stream-profile analysis and stream-gradient index

    Science.gov (United States)

    Hack, John T.

    1973-01-01

    The generally regular three-dimensional geometry of drainage networks is the basis for a simple method of terrain analysis providing clues to bedrock conditions and other factors that determine topographic forms. On a reach of any stream, a gradient-index value can be obtained which allows meaningful comparisons of channel slope on streams of different sizes. The index is believed to reflect stream power or competence and is simply the product of the channel slope at a point and channel length measured along the longest stream above the pointwhere the calculation is made. In an adjusted topography, changes in gradient-index values along a stream generally correspond to differences in bedrock or introduced load. In any landscape the gradient index of a stream is related to total relief and stream regimen. Thus, climate, tectonic events, and geomorphic history must be considered in using the gradient index. Gradient-index values can be obtained quickly by simple measurements on topographic maps, or they can be obtained by more sophisticated photogrammetric measurements that involve simple computer calculations from x, y, z coordinates.

  4. [Plasmid profile analysis in identification of epidemic strains of Salmonella enterica serovar Enteritidis].

    Science.gov (United States)

    Miljković-Selimović, Biljana; Lepsanović, Zorica; Babić, Tatjana; Kocić, Branislava; Randelović, Gordana

    2008-04-01

    As illness caused by Sallmonella enterica serovar Enteritidis (S. Enteritidis) occurs not only as sporadic cases but as outbreaks, to reveal the source and routes of spreading of infection it is necessary to identify epidemic strain by the use of some typing methods. To determine whether plasmid profile analysis, as genotyping method, could be applied for the investigation of epidemic strains, isolates of S. Enteritidis, recovered from patient's stools and food associated with outbreaks and those isolated from sporadic cases of diarrhea, were investigated. Investigation of antibiotic resistance was performed by Kirby-Bauer disc-diffusion method. Isolation of plasmid DNA was carried out by Birnboim and Dolly alkaline lysis method, modified by Ish-Horovitz. Out of 276 izolates of S. Enteritidis 94 were isolated from patient's stools and food associated with outbreaks and 182 were isolated from sporadic cases of diarrhea. The presence of 12 plasmid profiles was established. An average correlation degree of plasmid profiles between the strains was 0.84, that implies high degree of similarity of plasmid profiles of epidemic and non epidemic strains isolated at our geographic region for the given period of time. The strains of S. Enteritidis, isolated in outbreaks of enterocolitis as well as from spordic cases of diarrhea in the same period of time and at the same area, frequently exhibit the same plasmid profile characterized by a single plasmid of 38 MDa. Therefore, in most cases plasmid profile analysis is not valuable in the identification of epidemic strains of S. Enteritidis. However, for this purpose plasmid profile analysis could be used when drug-resistant strains of S. Enteritidis are isolated, as they often possess additional resistant plasmids what increases discrimination power of this method.

  5. Plasmid profile analysis in identification of epidemic strains of Salmonella enterica serovar Enteritidis

    Directory of Open Access Journals (Sweden)

    Miljković-Selimović Biljana

    2008-01-01

    Full Text Available Background/Aim. As illness caused by Sallmonella enterica serovar Enteritidis (S. Enteritidis occurs not only as sporadic cases but as outbreaks, to reveal the source and routes of spreading of infection it is necessary to identify epidemic strain by the use of some typing methods. To determine whether plasmid profile analysis, as genotyping method, could be applied for the investigation of epidemic strains, isolates of S. Enteritidis, recovered from patient's stools and food associated with outbreaks and those isolated from sporadic cases of diarrhea, were investigated. Methods. Investigation of antibiotic resistance was performed by Kirby - Bauer disc-diffusion method. Isolation of plasmid DNA was carried out by Birnboim and Dolly alkaline lysis method, modified by Ish-Horovitz. Results. Out of 276 izolates of S. Enteritidis 94 were isolated from patient's stools and food associated with outbreaks and 182 were isolated from sporadic cases of diarrhea. The presence of 12 plasmid profiles was established. An average correlation degree of plasmid profiles between the strains was 0.84, that implies high degree of similarity of plasmid profiles of epidemic and non- epidemic strains isolated at our geographic region for the given period of time. Conclusion. The strains of S. Enteritidis, isolated in outbreaks of enterocolitis as well as from spordic cases of diarrhea in the same period of time and at the same area, frequently exhibit the same plasmid profile characterized by a single plasmid of 38 MDa. Therefore, in most cases plasmid profile analysis is not valuable in the identification of epidemic strains of S. Enteritidis. However, for this purpose plasmid profile analysis could be used when drug-resistant strains of S. Enteritidis are isolated, as they often possess additional resistant plasmids what increases discrimination power of this method.

  6. Radiochemical procedures for analysis of Pu, Am, Cs and Sr in water, soil, sediments and biota samples

    Energy Technology Data Exchange (ETDEWEB)

    Wong, K.M.; Jokela, T.A.; Noshkin, V.E.

    1994-02-01

    The Environmental Radioactivity Analysis Laboratory (ERAL) was established as an analytical facility. The primary function of ERAL is to provide fast and accurate radiological data of environmental samples. Over the years, many radiochemical procedures have been developed by the staffs of ERAL. As result, we have found that our procedures exist in many different formats and in many different notebooks, documents and files. Therefore, in order to provide for more complete and orderly documentation of the radiochemical procedures that are being used by ERAL, we have decided to standardize the format and compile them into a series of reports. This first report covers procedures we have developed and are using for the radiochemical analysis of Pu, Am, Cs, and Sr in various matrices. Additional analytical procedures and/or revisions for other elements will be reported as they become available through continuation of these compilation efforts.

  7. Revisiting internal gravity waves analysis using GPS RO density profiles: comparison with temperature profiles and application for wave field stability study

    Directory of Open Access Journals (Sweden)

    P. Pisoft

    2018-01-01

    Full Text Available We revise selected findings regarding the utilization of Global Positioning System radio occultation (GPS RO density profiles for the analysis of internal gravity waves (IGW, introduced by Sacha et al. (2014. Using various GPS RO datasets, we show that the differences in the IGW spectra between the dry-temperature and dry-density profiles that were described in the previous study as a general issue are in fact present in one specific data version only. The differences between perturbations in the temperature and density GPS RO profiles do not have any physical origin, and there is not the information loss of IGW activity that was suggested in Sacha et al. (2014. We investigate the previously discussed question of the temperature perturbations character when utilizing GPS RO dry-temperature profiles, derived by integration of the hydrostatic balance. Using radiosonde profiles as a proxy for GPS RO, we provide strong evidence that the differences in IGW perturbations between the real and retrieved temperature profiles (which are based on the assumption of hydrostatic balance include a significant nonhydrostatic component that is present sporadically and might be either positive or negative. The detected differences in related spectra of IGW temperature perturbations are found to be mostly about ±10 %. The paper also presents a detailed study on the utilization of GPS RO density profiles for the characterization of the wave field stability. We have analyzed selected stability parameters derived from the density profiles together with a study of the vertical rotation of the wind direction. Regarding the Northern Hemisphere the results point to the western border of the Aleutian high, where potential IGW breaking is detected. These findings are also supported by an analysis of temperature and wind velocity profiles. Our results confirm advantages of the utilization of the density profiles for IGW analysis.

  8. Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples

    Directory of Open Access Journals (Sweden)

    Daniel Belstrøm

    2016-03-01

    Full Text Available Background and objective: The microbial profiles of stimulated saliva samples have been shown to differentiate between patients with periodontitis, patients with dental caries, and orally healthy individuals. Saliva was stimulated to allow for easy and rapid collection; however, microbial composition may not reflect the more natural, unstimulated state. The purpose of this study was to validate whether stimulated saliva is an adequate surrogate for unstimulated saliva in determining salivary microbiomes. Design: Unstimulated (n=20 and stimulated (n=20 saliva samples were collected from 20 orally and systemically healthy, non-smoking participants. Salivary bacterial profiles were analyzed by means of the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS, and statistical analysis was performed using Mann–Whitney test with Benjamini–Hochberg's correction for multiple comparison, cluster analysis, principal component analysis, and correspondence analysis. Results: From a total of 40 saliva samples, 496 probe targets were identified with a mean number of targets per sample of 203 (range: 146–303, and a mean number of probe targets of 206 and 200 in unstimulated and stimulated saliva samples, respectively (p=0.62. Based on all statistical methods used for this study, the microbial profiles of unstimulated and stimulated saliva samples collected from the same person were not statistically significantly different. Conclusions: Analysis of bacterial salivary profiles in unstimulated and stimulated saliva samples collected from the same individual showed comparable results. Thus, the results verify that stimulated saliva is an adequate surrogate of unstimulated saliva for microbiome-related studies.

  9. ANALYSIS OF PIG PROFILES ON SMALL-SCALE PIG FARMERS IN MANOKWARI-WEST PAPUA

    Directory of Open Access Journals (Sweden)

    D. A. Iyai

    2011-09-01

    Full Text Available This study was aimed to determine the profiles of pig farming systems. Participatory situation analysis was employed to gain data relating to pig profiles in the urban and rural areas of Manokwari. Due to the interests of combining many correlated data, multivariate analysis using Principal Component Analysis (PCA was performed. Cluster Analysis using Agglomerative Hierarchical Clustering was applied for analysis of merge samples based on similarity in components’ composition across sites. There were various twelve classes of pig profiles in Manokwari. In principal component of the first axis correlation of several components shows strong positive relation e.g. in piglets, sows, and total herd size. Status of region in the first axis of PCA (P1 through which pigs were raised had negative correlation, including grower, household member and pig production. In the second axis (P2, negative correlations were shown in piglets, grower, boars, total herd size and the Pig Production Potential (PPP. Status of region has underpinned profiles of pigs. Several farmers were able to manage their farm continuity in a steady composition. Selling pigs was the main aim and few farmers play a role as pure breeder.

  10. Friedel's salt profiles from thermogravimetric analysis and thermodynamic modelling of Portland cement-based mortars exposed to sodium chloride solution

    DEFF Research Database (Denmark)

    Shi, Zhenguo; Geiker, Mette Rica; Lothenbach, Barbara

    2017-01-01

    Thermogravimetric analysis (TGA), powder X-ray diffraction (XRD) and thermodynamic modelling have been used to obtain Friedel's salt profiles for saturated mortar cylinders exposed to a 2.8 M NaCl solution. Comparison of the measured Friedel's salt profiles with the total chloride profiles...

  11. Combined Radar and Radiometer Analysis of Precipitation Profiles for a Parametric Retrieval Algorithm

    Science.gov (United States)

    Masunaga, Hirohiko; Kummerow, Christian D.

    2005-01-01

    A methodology to analyze precipitation profiles using the Tropical Rainfall Measuring Mission (TRMM) Microwave Imager (TMI) and precipitation radar (PR) is proposed. Rainfall profiles are retrieved from PR measurements, defined as the best-fit solution selected from precalculated profiles by cloud-resolving models (CRMs), under explicitly defined assumptions of drop size distribution (DSD) and ice hydrometeor models. The PR path-integrated attenuation (PIA), where available, is further used to adjust DSD in a manner that is similar to the PR operational algorithm. Combined with the TMI-retrieved nonraining geophysical parameters, the three-dimensional structure of the geophysical parameters is obtained across the satellite-observed domains. Microwave brightness temperatures are then computed for a comparison with TMI observations to examine if the radar-retrieved rainfall is consistent in the radiometric measurement space. The inconsistency in microwave brightness temperatures is reduced by iterating the retrieval procedure with updated assumptions of the DSD and ice-density models. The proposed methodology is expected to refine the a priori rain profile database and error models for use by parametric passive microwave algorithms, aimed at the Global Precipitation Measurement (GPM) mission, as well as a future TRMM algorithms.

  12. Granulometric profiling of aeolian dust deposits by automated image analysis

    Science.gov (United States)

    Varga, György; Újvári, Gábor; Kovács, János; Jakab, Gergely; Kiss, Klaudia; Szalai, Zoltán

    2016-04-01

    Determination of granulometric parameters is of growing interest in the Earth sciences. Particle size data of sedimentary deposits provide insights into the physicochemical environment of transport, accumulation and post-depositional alterations of sedimentary particles, and are important proxies applied in paleoclimatic reconstructions. It is especially true for aeolian dust deposits with a fairly narrow grain size range as a consequence of the extremely selective nature of wind sediment transport. Therefore, various aspects of aeolian sedimentation (wind strength, distance to source(s), possible secondary source regions and modes of sedimentation and transport) can be reconstructed only from precise grain size data. As terrestrial wind-blown deposits are among the most important archives of past environmental changes, proper explanation of the proxy data is a mandatory issue. Automated imaging provides a unique technique to gather direct information on granulometric characteristics of sedimentary particles. Granulometric data obtained from automatic image analysis of Malvern Morphologi G3-ID is a rarely applied new technique for particle size and shape analyses in sedimentary geology. Size and shape data of several hundred thousand (or even million) individual particles were automatically recorded in this study from 15 loess and paleosoil samples from the captured high-resolution images. Several size (e.g. circle-equivalent diameter, major axis, length, width, area) and shape parameters (e.g. elongation, circularity, convexity) were calculated by the instrument software. At the same time, the mean light intensity after transmission through each particle is automatically collected by the system as a proxy of optical properties of the material. Intensity values are dependent on chemical composition and/or thickness of the particles. The results of the automated imaging were compared to particle size data determined by three different laser diffraction instruments

  13. A comparative analysis of British and Taiwanese students' conceptual and procedural knowledge of fraction addition

    Science.gov (United States)

    Li, Hui-Chuan

    2014-10-01

    This study examines students' procedural and conceptual achievement in fraction addition in England and Taiwan. A total of 1209 participants (561 British students and 648 Taiwanese students) at ages 12 and 13 were recruited from England and Taiwan to take part in the study. A quantitative design by means of a self-designed written test is adopted as central to the methodological considerations. The test has two major parts: the concept part and the skill part. The former is concerned with students' conceptual knowledge of fraction addition and the latter is interested in students' procedural competence when adding fractions. There were statistically significant differences both in concept and skill parts between the British and Taiwanese groups with the latter having a higher score. The analysis of the students' responses to the skill section indicates that the superiority of Taiwanese students' procedural achievements over those of their British peers is because most of the former are able to apply algorithms to adding fractions far more successfully than the latter. Earlier, Hart [1] reported that around 30% of the British students in their study used an erroneous strategy (adding tops and bottoms, for example, 2/3 + 1/7 = 3/10) while adding fractions. This study also finds that nearly the same percentage of the British group remained using this erroneous strategy to add fractions as Hart found in 1981. The study also provides evidence to show that students' understanding of fractions is confused and incomplete, even those who are successfully able to perform operations. More research is needed to be done to help students make sense of the operations and eventually attain computational competence with meaningful grounding in the domain of fractions.

  14. Development of a Quality Assurance Procedure for Dose Volume Histogram Analysis

    Science.gov (United States)

    Davenport, David A.

    The role of the dose-volume histogram (DVH) is rapidly expanding in radiation oncology treatment planning. DVHs are already relied upon to differentiate between two similar plans and evaluate organ-at-risk dosage. Their role will become even more important as progress continues towards implementing biologically based treatment planning systems. Therefore it is imperative that the accuracy of DVHs is evaluated and reappraised after any major software or hardware upgrades, affecting a treatment planning system (TPS). The purpose of this work is to create and implement a comprehensive quality assurance procedure evaluating dose volume histograms to insure their accuracy while satisfying American College of Radiology guidelines. Virtual phantoms of known volumes were created in Pinnacle TPS and exposed to different beam arrangements. Variables including grid size and slice thickness were varied and their effects were analyzed. The resulting DVHs were evaluated by comparison to the commissioned percent depth dose values using a custom Excel spreadsheet. After determining the uncertainty of the DVH based on these variables, multiple second check calculations were performed using MIM Maestro and Matlab software packages. The uncertainties of the DVHs were shown to be less than +/- 3%. The average uncertainty was shown to be less than +/- 1%. The second check procedures resulted in mean percent differences less than 1% which confirms the accuracy of DVH calculation in Pinnacle and the effectiveness of the quality assurance template. The importance of knowing the limits of accuracy of the DVHs, which are routinely used to assess the quality of clinical treatment plans, cannot be overestimated. The developed comprehensive QA procedure evaluating the accuracy of the DVH statistical analysis will become a part of our clinical arsenal for periodic tests of the treatment planning system. It will also be performed at the time of commissioning and after any major software

  15. [Marginal adaptation after cementing of gold inlays cast by an experimental procedure. SEM analysis].

    Science.gov (United States)

    Traini, T; Di Iorio, D; Murmura, G; Caputi, S

    2004-03-01

    Marginal adaptation is one of the most important factors in dental reconstructions. The aim of the present work was to evaluate with in vivo and in vitro studies the marginal precision and the microstructure of the gold cast inlays. For the in vivo analysis 10 patients were selected, while for the in vitro study 10 extracted teeth were used. The impressions were made by polyvinylsiloxane while the master cast was made by gypsum type IV. The inlays were cast in gold (JRVT Jensen) using an experimental procedure. After cementing, the margins of each inlay was burnished. To evaluate the marginal discrepancy of in vivo inlays, the replica technique was used, while for the in vitro analysis the serial slice cut technique was performed. All samples were processed for metallographic analysis using SEM. The measured in vivo inlay marginal gap was 9,06+/-3,37 microns (mean+/-SD), while for in vitro inlay the marginal gap was of 10,47+/-2,09 microns (mean+/-SD). Metallographic analysis showed equiaxial grain of small dimension. The results suggest that using the proposed casting technique it is possible to increase marginal adaptation and microstructural properties.

  16. Algebraic analysis of the phase-calibration problem in the self-calibration procedures

    Science.gov (United States)

    Lannes, A.; Prieur, J.-L.

    2011-10-01

    This paper presents an analysis of the phase-calibration problem encountered in astronomy when mapping incoherent sources with aperture-synthesis devices. More precisely, this analysis concerns the phase-calibration operation involved in the self-calibration procedures of phase-closure imaging. The paper revisits and completes a previous analysis presented by Lannes in the Journal of the Optical Society of America A in 2005. It also benefits from some recent developments made for solving similar problems encountered in global navigation satellite systems. In radio-astronomy, the related optimization problems have been stated and solved hitherto at the phasor level. We present here an analysis conducted at the phase level, from which we derive a method for diagnosing and solving the difficulties of the phasor approach. In the most general case, the techniques to be implemented appeal to the algebraic graph theory and the algebraic number theory. The minima of the objective functionals to be minimized are identified by raising phase-closure integer ambiguities. We also show that in some configurations, to benefit from all the available information, closure phases of order greater than three are to be introduced. In summary, this study leads to a better understanding of the difficulties related to the very principle of phase-closure imaging. To circumvent these difficulties, we propose a strategy both simple and robust.

  17. Problems of the Procedure for Regulatory Impact Analysis of Draft Normative Legal Acts

    Directory of Open Access Journals (Sweden)

    Ivanova Olga Yu.

    2017-06-01

    Full Text Available The aim of the article is to investigate the procedure for regulatory impact analysis and identify problems caused by the adoption of ineffective normative legal acts. The essence of the concept “regulatory impact analysis” and its main tasks are considered. The legislation in the field of RIA is studied, and the contradictions in terms of determining the number of stages of analysis and the timing of the baseline performance monitoring revealed. There made conclusions regarding the unsatisfactory quality of the developed RIAs and presence of violations of legislation requirements by regulatory bodies, mandatory promulgation of the draft normative legal act along with the analysis. There identified the most problematic stages of RIA for the developers, which include identification of mandatory performance indicators, evaluation of compliance with the requirements of the regulatory act and definition of the problem. It is suggested to improve the methodology for regulatory impact analysis by completing the list of its main stages with evaluation of quality and adjustment of RIA. Recommendations for eliminating contradictions in the legislation in the field of RIA are developed.

  18. ANALYSIS OF MOTIVATIONAL PROFILES OF SATISFACTION AND IMPORTANCE OF PHYSICAL EDUCATION IN HIGH SCHOOL ADOLESCENTS

    Directory of Open Access Journals (Sweden)

    Antonio Granero-Gallegos

    2012-12-01

    Full Text Available he purpose of this study was to analyze the motivational profiles of satisfaction with and importance of physical education in high school students and its relation with gender and the practice of sport. The sample comprised 2002 students aged from 12 to 19 who completed the Sport Motivation Scale (Núñez et al., 2006, the Perception of Success Scale (Martínez et al., 2006, the Sport Satisfaction Instrument (Baena-Extremera et al., 2012 and the Importance of Physical Education Scale (Moreno et al., 2009. Descriptive analyzes, correlations between the scales, a cluster analysis for profiles, and a MANOVA were conducted to examine differences by gender. Three clusters (profiles were identified. The first profile identified was "moderate" motivation (n = 463 and was associated with boys who practice physical activity for less than 3 hours per week. The second profile identified was "low" motivation (n = 545 and was associated mainly with girls who practice physical activity for less than 3 hours per week. And lastly the third profile identified was "high" motivation (n = 910, which was found to be greater in boys who practiced physical exercise for more than 3 hours a week

  19. Study on error analysis and accuracy improvement for aspheric profile measurement

    Science.gov (United States)

    Gao, Huimin; Zhang, Xiaodong; Fang, Fengzhou

    2017-06-01

    Aspheric surfaces are important to the optical systems and need high precision surface metrology. Stylus profilometry is currently the most common approach to measure axially symmetric elements. However, if the asphere has the rotational alignment errors, the wrong cresting point would be located deducing the significantly incorrect surface errors. This paper studied the simulated results of an asphere with rotational angles around X-axis and Y-axis, and the stylus tip shift in X, Y and Z direction. Experimental results show that the same absolute value of rotational errors around X-axis would cause the same profile errors and different value of rotational errors around Y-axis would cause profile errors with different title angle. Moreover, the greater the rotational errors, the bigger the peak-to-valley value of profile errors. To identify the rotational angles in X-axis and Y-axis, the algorithms are performed to analyze the X-axis and Y-axis rotational angles respectively. Then the actual profile errors with multiple profile measurement around X-axis are calculated according to the proposed analysis flow chart. The aim of the multiple measurements strategy is to achieve the zero position of X-axis rotational errors. Finally, experimental results prove the proposed algorithms achieve accurate profile errors for aspheric surfaces avoiding both X-axis and Y-axis rotational errors. Finally, a measurement strategy for aspheric surface is presented systematically.

  20. Anodic stripping voltammetry procedure modified for improved accuracy of blood lead analysis.

    Science.gov (United States)

    Roda, S M; Greenland, R D; Bornschein, R L; Hammond, P B

    1988-03-01

    In evaluating the accuracy and reliability of blood lead (PbB) measurements with the Environmental Science Associates Model 3010A Trace Metal Analyzer, intralaboratory comparison demonstrated that use of the operating conditions recommended by the manufacturer resulted in consistently underestimated PbB concentrations less than 400 micrograms/L and overestimated PbB values greater than 400 micrograms/L. At PbB concentrations less than 50 micrograms/L, measured concentrations were often registered as negative results. However, these negative values could be replicated to within +/- 10 micrograms/L, indicating good precision of the method, but obviously not good accuracy. In addition, lower-than-expected lead (Pb) values were measured in samples containing increased concentrations of copper (Cu), such as may occur in pregnant women. We modified the procedure to eliminate these inaccuracies by substituting manual peak-height measurements for reliance on the integrator and digital display of the instrument. We established the accuracy of the modified procedure by using calibration standards previously quantified by isotope dilution-mass spectroscopy. A quality-control program for monitoring PbB analysis is also described.

  1. A Bloch-based procedure for dispersion analysis of lattices with periodic time-varying properties

    Science.gov (United States)

    Vila, Javier; Pal, Raj Kumar; Ruzzene, Massimo; Trainiti, Giuseppe

    2017-10-01

    We present a procedure for the systematic estimation of the dispersion properties of linear discrete systems with periodic time-varying coefficients. The approach relies on the analysis of a single unit cell, making use of Bloch theorem along with the application of a harmonic balance methodology over an imposed solution ansatz. The solution of the resulting eigenvalue problem is followed by a procedure that selects the eigen-solutions corresponding to the ansatz, which is a plane wave defined by a frequency-wavenumber pair. Examples on spring-mass superlattices demonstrate the effectiveness of the method at predicting the dispersion behavior of linear elastic media. The matrix formulation of the problem suggests the broad applicability of the proposed technique. Furthermore, it is shown how dispersion can inform about the dynamic behavior of time-modulated finite lattices. The technique can be extended to multiple areas of physics, such as acoustic, elastic and electromagnetic systems, where periodic time-varying material properties may be used to obtain non-reciprocal wave propagation.

  2. A procedure for isolating social desirability variance in a three-way component analysis.

    Science.gov (United States)

    Lorenzo-Seva, Urbano; Ferrando, Pere J

    2012-02-01

    Three-way component analysis aims to summarize all the information in a three-way data set related, for example, to the responses of individuals to a set of items in a set of situations. The data are decomposed into three component matrices (person, item and situation components) and a core matrix. Such data are generally obtained by means of a situation-response questionnaire administered to a sample of participants. This paper proposes a preprocessing procedure for controlling social desirability in these kinds of questionnaires. The essential idea is to isolate the variance due to social desirability response bias and remove it from the data set. The procedure is demonstrated through its empirical application in the personality domain. Its performance is also assessed by means of a simulation study which shows that the presence of social desirability bias in a data set had different consequences: (1) the variance effect due to persons increased, and the effect of the triple interaction persons × items × situations decreased; (2) the person component and core matrix were partially distorted. However, the social desirability bias in the data set was successfully controlled by our preprocessing method. © 2011 The British Psychological Society.

  3. Soft tissue substitutes in non-root coverage procedures: a systematic review and meta-analysis.

    Science.gov (United States)

    Bertl, Kristina; Melchard, Maximilian; Pandis, Nikolaos; Müller-Kern, Michael; Stavropoulos, Andreas

    2017-03-01

    The present systematic review compared the effectiveness of soft tissue substitutes (STSs) and autogenous free gingival grafts (FGGs) in non-root-coverage procedures to increase keratinized tissue (KT) width around teeth. Included studies fulfilled the following main eligibility criteria: (a) preclinical in vivo or human controlled trials using FGG as control, (b) non-root-coverage procedures, and (c) assessment of KT width. Meta-analysis was performed on the gain in KT width (primary outcome variable) and several secondary variables. Eight human trials with short observation time evaluating five different STSs were identified. FGG yielded consistently significantly (p FGG yielded consistently ≥2 mm KT width postoperatively, while use of STS did not, in the few studies reporting on this outcome. On the other hand, STSs resulted in significantly better aesthetic outcomes and received greater patient preference (p FGG (1) resulted consistently in significantly larger increase in KT width compared to STS and (2) yielded consistently ≥2 mm KT width postoperatively, while STSs did not. STSs yielded significantly better aesthetic outcomes, received greater patient preference, and appeared safe. Larger and more predictable increase in KT width is achieved with FGG, but STSs may be considered when aesthetics is important. Clinical studies reporting relevant posttreatment outcomes, e.g., postop KT width ≥2 mm, on the long-term (>6 months) are warranted.

  4. Psychological profiles in patients with Sjögren's syndrome related to fatigue: a cluster analysis.

    Science.gov (United States)

    van Leeuwen, Ninke; Bossema, Ercolie R; Knoop, Hans; Kruize, Aike A; Bootsma, Hendrika; Bijlsma, Johannes W J; Geenen, Rinie

    2015-05-01

    Fatigue is a highly prevalent and debilitating symptom in the autoimmune disease SS. Although the disease process plays a role in fatigue, psychological factors may influence fatigue and the ability to deal with its consequences. Profiles of co-occurring psychological factors may suggest potential targets for the treatment of fatigue. The aim of this study was to identify psychological profiles in patients with SS and the accompanying levels of fatigue. Three hundred patients with primary SS (mean age 57 years, 93% female) completed questionnaires on fatigue (multidimensional fatigue inventory), physical activity cognitions (TAMPA-SK), illness cognitions, cognitive regulation, emotion processing and regulation [Toronto Alexithymia Scale 20, Emotion Regulation Questionnaire (ERQ), Berkeley Expressivity Questionnaire], coping strategies (Brief COPE) and social support. Principal axis factor analysis (oblimin rotation) yielded six psychological factors: social support, negative thinking, positive thinking, emotional expressivity, avoidance and alexithymia (i.e. the inability to differentiate emotions). Using cluster analyses, these factors were grouped in four psychological profiles: functional (39%), alexithymic (27%), self-reliant (23%) and dysfunctional (11%). Irrespective of the psychological profile, the level of fatigue was substantially higher in patients than in the general population. Patients with a dysfunctional or an alexithymic profile reported more fatigue than those with a self-reliant profile. Our study in SS yielded four psychological profiles that were differentially associated with fatigue. These profiles can be used to examine determinants and prognosis of fatigue as well as the possibility of customizing cognitive behavioural interventions for chronic fatigue. © The Author 2014. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  5. Seismic profile analysis of the Kangra and Dehradun re-entrant of ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Earth System Science; Volume 126; Issue 6. Seismic profile analysis of the Kangra ... Here we use a predictive angular function for establishing quantitative geometric relationships between fault and fold shapes with 'Distance–displacement method' (D–d method). It is a prognostic straightforward ...

  6. The cognitive profile of ALS: a systematic review and meta-analysis update

    NARCIS (Netherlands)

    Beeldman, E.; Raaphorst, J.; Klein Twennaar, M.; Visser, M de; Schmand, B.A.; Haan, R.J. de

    2016-01-01

    Cognitive impairment is present in approximately 30% of patients with amyotrophic lateral sclerosis (ALS) and, especially when severe, has a negative impact on survival and caregiver burden. Our 2010 meta-analysis of the cognitive profile of ALS showed impairment of fluency, executive function,

  7. A Latent Profile Analysis of University Students' Self-Regulated Learning Strategies

    Science.gov (United States)

    Ning, Hoi Kwan; Downing, Kevin

    2015-01-01

    Based on self-reported cognitive, metacognitive, and behavioural strategy measures obtained from 828 final-year students from a university in Hong Kong, latent profile analysis (LPA) identified four distinct types of students with differential self-regulated learning strategy orientations: "competent self-regulated learners",…

  8. Social-Emotional Functioning of Elementary-Age Deaf Children: A Profile Analysis

    Science.gov (United States)

    Vogel-Walcutt, Jennifer J.; Schatschneider, Christopher; Bowers, Clint

    2011-01-01

    Discussion and study of the social-emotional development of deaf and hard of hearing children, though extensive, has yet to provide an accurate understanding of the differences between deaf and hearing children. Consequently, the goal of the researchers was to conduct a profile analysis to determine similarities and differences between the two…

  9. PIC. Profile of Interaction in the Classroom. A Quick Feedback of Interaction Analysis.

    Science.gov (United States)

    Brunner, Ellen

    The Profile of Interaction in the Classroom (PIC) is a short-cut method of interaction analysis that can provide the quick feedback essential to effective supervision of instruction. And because the PIC contains a record of all the behaviors that occurred in the classroom, as well as the sequence, the data may be used to build a traditional…

  10. Student Motivational Profiles in an Introductory MIS Course: An Exploratory Cluster Analysis

    Science.gov (United States)

    Nelson, Klara

    2014-01-01

    This study profiles students in an introductory MIS course according to a variety of variables associated with choice of academic major. The data were collected through a survey administered to 12 sections of the course. A two-step cluster analysis was performed with gender as a categorical variable and students' perceptions of task value…

  11. Examining Early Literacy Skill Differences among Children in Head Start via Latent Profile Analysis

    Science.gov (United States)

    Norwalk, Kate E.; DiPerna, James C.; Lei, Pui-wa; Wu, Qiong

    2012-01-01

    The purpose of the present study was to determine whether there are systematic differences in literacy skills among children from less-advantaged households, using latent profile analysis. Early reading skills were measured using the Early Arithmetic, Reading, and Learning Indicators (EARLI; DiPerna, Morgan, & Lei, 2007) literacy tasks.…

  12. A Latent Profile Analysis of Math Achievement, Numerosity, and Math Anxiety in Twins

    Science.gov (United States)

    Hart, Sara A.; Logan, Jessica A. R.; Thompson, Lee; Kovas, Yulia; McLoughlin, Gráinne; Petrill, Stephen A.

    2016-01-01

    Underperformance in math is a problem with increasing prevalence, complex etiology, and severe repercussions. This study examined the etiological heterogeneity of math performance in a sample of 264 pairs of 12-year-old twins assessed on measures of math achievement, numerosity, and math anxiety. Latent profile analysis indicated 5 groupings of…

  13. An analysis of the reading profiles of first-year students at ...

    African Journals Online (AJOL)

    Erna Kinsey

    Many South African students enter higher education under-prepared for the reading demands that are placed upon them. These students very often become part of the "revolving door syndrome". An analysis of the reading assessment profiles of a group of first-year students at Potchefstroom University indicated that these ...

  14. An analysis of reading profiles of first-year students at Potchefstroom ...

    African Journals Online (AJOL)

    These students very often become part of the "revolving door syndrome". An analysis of the reading assessment profiles of a group of first-year students at Potchefstroom University indicated that these students experienced problems across all aspects of the reading process (i.e. vocabulary, fluency, reading comprehension, ...

  15. The Deficit Profiles of Chinese Children with Reading Difficulties: A Meta-Analysis

    Science.gov (United States)

    Peng, Peng; Wang, Cuicui; Tao, Sha; Sun, Congying

    2017-01-01

    The current meta-analysis synthesized findings from profiling research on Chinese children with reading difficulties (RD). We reviewed a total of 81 studies published between 1964 and May 2015, representing a total of 9735 Chinese children. There are 982 effect sizes for the comparison between children with RD and age-matched typically developing…

  16. Fertilizer/Chemical Sales and Service Worker. Ohio's Competency Analysis Profile.

    Science.gov (United States)

    Ohio State Univ., Columbus. Vocational Instructional Materials Lab.

    This Ohio Competency Analysis Profile (OCAP), derived from a modified Developing a Curriculum (DACUM) process, is a current comprehensive and verified employer competency program list for fertilizer/chemical sales and service workers. Each unit (with or without subunits) contains competencies and competency builders that identify the occupational,…

  17. Latent Profile Analysis to Determine the Typology of Disinhibited Eating Behaviors in Children and Adolescents

    Science.gov (United States)

    Vannucci, Anna; Tanofsky-Kraff, Marian; Crosby, Ross D.; Ranzenhofer, Lisa M.; Shomaker, Lauren B.; Field, Sara E.; Mooreville, Mira; Reina, Samantha A.; Kozlosky, Merel; Yanovski, Susan Z.; Yanovski, Jack A.

    2013-01-01

    Objective: We used latent profile analysis (LPA) to classify children and adolescents into subtypes based on the overlap of disinhibited eating behaviors--eating in the absence of hunger, emotional eating, and subjective and objective binge eating. Method: Participants were 411 youths (8-18 years) from the community who reported on their…

  18. A New Tool for Discourse Analysis: The Vocabulary-Management Profile.

    Science.gov (United States)

    Youmans, Gilbert

    1991-01-01

    Proposes the Vocabulary-Management Profile, a tool for discourse analysis. The number of new words introduced in a moving interval of text 35 words long is counted and a curve created by plotting the number of new words in a successive interval at the midpoint of the interval. Analyses of text by George Orwell and James Joyce are presented. (JL)

  19. Subgrouping of Readers Based on Performance Measures: A Latent Profile Analysis

    Science.gov (United States)

    Wolff, Ulrika

    2010-01-01

    By using latent profile analysis eight stable and interpretable subgroups of readers were identified. The basis for subgrouping was different performance measures with four aspects of reading in focus: reading of continuous texts, reading of document texts, word reading and reading speed. Participants were 9-year-old Swedish students included in…

  20. Three subgroups of pain profiles identified in 227 women with arthritis: a latent class analysis.

    Science.gov (United States)

    de Luca, Katie; Parkinson, Lynne; Downie, Aron; Blyth, Fiona; Byles, Julie

    2017-03-01

    The objectives were to identify subgroups of women with arthritis based upon the multi-dimensional nature of their pain experience and to compare health and socio-demographic variables between subgroups. A latent class analysis of 227 women with self-reported arthritis was used to identify clusters of women based upon the sensory, affective, and cognitive dimensions of the pain experience. Multivariate multinomial logistic regression analysis was used to determine the relationship between cluster membership and health and sociodemographic characteristics. A three-class cluster model was most parsimonious. 39.5 % of women had a unidimensional pain profile; 38.6 % of women had moderate multidimensional pain profile that included additional pain symptomatology such as sensory qualities and pain catastrophizing; and 21.9 % of women had severe multidimensional pain profile that included prominent pain symptomatology such as sensory and affective qualities of pain, pain catastrophizing, and neuropathic pain. Women with severe multidimensional pain profile have a 30.5 % higher risk of poorer quality of life and a 7.3 % higher risk of suffering depression, and women with moderate multidimensional pain profile have a 6.4 % higher risk of poorer quality of life when compared to women with unidimensional pain. This study identified three distinct subgroups of pain profiles in older women with arthritis. Women had very different experiences of pain, and cluster membership impacted significantly on health-related quality of life. These preliminary findings provide a stronger understanding of profiles of pain and may contribute to the development of tailored treatment options in arthritis.

  1. Flash Profile for rapid descriptive analysis in sensory characterization of passion fruit juice

    Directory of Open Access Journals (Sweden)

    Flávia Daiana Montanuci

    2015-07-01

    Full Text Available The Flash Profile is a descriptive analysis method derived from Free-Choice Profile, in which each taster chooses and uses his/her own words to evaluate the product while comparing several attributes. Four passion fruit juices were analyzed, two juices were produced with concentrated juice, one with pulp and one with reconstituted juice; all juices had different levels of sugar, some had gum and dyes. This study aimed to evaluate the physicochemical properties (color, titratable acidity and solid content as well as sensory analysis like Flash profile and affective test. In physicochemical characterization and in Flash Profile, the juice A (pulp had higher solid content and consistence, the juice B (concentrated juice was the least acidic and presented the lowest value of soluble solids and presented strong aroma and flavor of passion-fruit, the juice C (reconstituted juice was pale yellow and showed artificial flavor and the juice D (concentrated juice was the most acidic, consistent with the natural flavor. In the acceptance test, all the juices scored 5-6, indicating that panelists tasters neither liked nor disliked. Flash Profile proved to be an easy and rapid technique showing a good correlation between panelists and the attributes and confirmed the results of physicochemical characterization.

  2. An Analysis of First-Grade Writing Profiles and Their Relationship to Compositional Quality.

    Science.gov (United States)

    Coker, David L; Ritchey, Kristen D; Uribe-Zarain, Ximena; Jennings, Austin S

    2017-05-01

    To help all students meet the writing expectations of the Common Core State Standards, researchers need a deeper understanding of the characteristics of struggling writers. The purpose of this study was to explore the writing profiles of students including those who have or are at risk for writing disabilities. First-grade students ( N = 391) were assessed at the end of the school year using three writing assessments (spelling, sentence writing fluency, writing achievement). The researchers used latent profile analysis to identify students as fitting into one of five profiles (At Risk, Low Fluency, Low Writing, Average, and Above Average). Students also wrote narrative and descriptive texts that were scored multiple ways. The researchers used confirmatory factor analysis to identify four common factors: quality/length, spelling, mechanics, and syntax. Students in the At Risk profile wrote narratives and descriptions that scored lower on all aspects of writing when compared to students in the Average and Above Average profiles. These findings provide further evidence of the distinct difference among writers as early as first grade, and they offer insight into the characteristics of at-risk writers. The implications of these findings for instruction and assessment and directions for future research are described.

  3. Automatic procedure for the correction of thermoelastic stress analysis data acquired in nonadiabatic conditions

    Science.gov (United States)

    Gallotti, A.; Salerno, A.

    2005-12-01

    Thermoelastic stress analysis (TSA), performed on metallic components with a high diffusivity coefficient, seldom reaches adiabatic conditions. As a consequence, TSA results are affected by an attenuation whose entity varies locally, preventing the use of TSA as a reliable quantitative investigation means. The recovery of the adiabatic temperature and of the correct value of the first stress invariant, directly linked to it, can only be performed making an assumption on the local stress distribution. This article presents a method for automatically choosing, among a number of stress distribution functions, the one that performs the best correction of the raw TSA data. The implementation of this automatic correction procedure in a computer program allowed the point-by-point correction of whole TSA images.

  4. Experimental and theoretical analysis on the procedure for estimating geo-stresses by the Kaiser effect

    Science.gov (United States)

    Li, Yuan-Hui; Yang, Yu-Jiang; Liu, Jian-Po; Zhao, Xing-Dong

    2010-10-01

    Acoustic emission tests of the core specimens retrieved from boreholes at the depth over 1000 m in Hongtoushan Copper Mine were carried out under uniaxial compressive loading, and the numerical test was also done by using the rock failure process analysis (RFPA2D) code, based on the procedure for estimating geo-stresses by the Kaiser effect under uniaxial compression. According to the statistical damage mechanics theory, the Kaiser effect mechanism was analyzed. Based on these analyses, it is indicted that the traditional method of estimating geo-stresses by the Kaiser effect is not appropriate, and the result is usually smaller than the real one. Furthermore, the greater confining compression in the rock mass may result in a larger difference between the Kaiser effect stresses acquired from uniaxial loading in laboratory and the real in-situ stresses.

  5. Immunohistochemistry profiles of breast ductal carcinoma: factor analysis of digital image analysis data

    Science.gov (United States)

    2012-01-01

    variation. Finally, we confirmed high expression levels of p16 in Triple-negative tumours. Conclusion Factor analysis of multiple IHC biomarkers measured by automated DA is an efficient exploratory tool clarifying complex interdependencies in the breast ductal carcinoma IHC profiles and informative value of single IHC markers. Integrated IHC indices may provide additional risk stratifications for the currently used grading systems and prove to be useful in clinical outcome studies. Virtual Slides The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1512077125668949 PMID:22424533

  6. Immunohistochemistry profiles of breast ductal carcinoma: factor analysis of digital image analysis data

    Directory of Open Access Journals (Sweden)

    Laurinavicius Arvydas

    2012-03-01

    the major factor of variation. Finally, we confirmed high expression levels of p16 in Triple-negative tumours. Conclusion Factor analysis of multiple IHC biomarkers measured by automated DA is an efficient exploratory tool clarifying complex interdependencies in the breast ductal carcinoma IHC profiles and informative value of single IHC markers. Integrated IHC indices may provide additional risk stratifications for the currently used grading systems and prove to be useful in clinical outcome studies. Virtual Slides The virtual slide(s for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1512077125668949

  7. Maori heads (mokomokai): the usefulness of a complete forensic analysis procedure.

    Science.gov (United States)

    Charlier, Philippe; Huynh-Charlier, Isabelle; Brun, Luc; Champagnat, Julie; Laquay, Laetitia; Hervé, Christian

    2014-09-01

    Based on an analysis of 19 mummified Maori heads (mokomokai) referred to our forensic laboratory for anthropological analysis prior to their official repatriation from France to New Zealand, and data from the anthropological and medical literature, we propose a complete forensic procedure for the analysis of such pieces. A list of 12 original morphological criteria was developed. Items included the sex, age at death, destruction of the skull base, the presence of argil deposits in the inner part of the skull, nostrils closed with exogenous material, sewing of eyelids and lips, pierced earlobes, ante-mortem and/or post-mortem tattoos, the presence of vegetal fibers within nasal cavities, and other pathological or anthropological anomalies. These criteria were tested for all 19 mokomokai repatriated to New Zealand by the French authorities. Further complementary analyses were limited to fiberscopic examination of the intracranial cavities because of the taboo on any sampling requested by the Maori authorities. In the context of global repatriation of human artifacts to native communities, this type of anthropological expertise is increasingly frequently requested of forensic anthropologists and other practitioners. We discuss the reasons for and against repatriating non-authentic artifacts to such communities and the role played by forensic anthropologists during the authentication process.

  8. A single extraction and HPLC procedure for simultaneous analysis of phytosterols, tocopherols and lutein in soybeans.

    Science.gov (United States)

    Slavin, Margaret; Yu, Liangli Lucy

    2012-12-15

    A saponification/extraction procedure and high performance liquid chromatography (HPLC) analysis method were developed and validated for simultaneous analysis of phytosterols, tocopherols and lutein (a carotenoid) in soybeans. Separation was achieved on a phenyl column with a ternary, isocratic solvent system of acetonitrile, methanol and water (48:22.5:29.5, v/v/v). Evaporative light scattering detection (ELSD) was used to quantify β-sitosterol, stigmasterol, campesterol, and α-, δ- and γ-tocopherols, while lutein was quantified with visible light absorption at 450 nm. Peak identification was verified by retention times and spikes with external standards. Standard curves were constructed (R(2)>0.99) to allow for sample quantification. Recovery of the saponification and extraction was demonstrated via analysis of spiked samples. Also, the accuracy of results of four soybeans using the described saponification and HPLC analytical method was validated against existing methods. This method offers a more efficient alternative to individual methods for quantifying lutein, tocopherols and sterols in soybeans. Copyright © 2012 Elsevier Ltd. All rights reserved.

  9. A continuous OSL scanning method for analysis of radiation depth-dose profiles in bricks

    DEFF Research Database (Denmark)

    Bøtter-Jensen, L.; Jungner, H.; Poolton, N.R.J.

    1995-01-01

    This article describes the development of a method for directly measuring radiation depth-dose profiles from brick, tile and porcelain cores, without the need for sample separation techniques. For the brick cores, examples are shown of the profiles generated by artificial irradiation using...... the different photon energies from Cs-137 and Co-60 gamma sources; comparison is drawn with both the theoretical calculations derived from Monte Carlo simulations, as well as experimental measurements made using more conventional optically stimulated luminescence methods of analysis....

  10. Systematic analysis of Ca2+ homeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles

    Science.gov (United States)

    Ghanegolmohammadi, Farzan; Yoshida, Mitsunori; Ohnuki, Shinsuke; Sukegawa, Yuko; Okada, Hiroki; Obara, Keisuke; Kihara, Akio; Suzuki, Kuninori; Kojima, Tetsuya; Yachie, Nozomu; Hirata, Dai; Ohya, Yoshikazu

    2017-01-01

    We investigated the global landscape of Ca2+ homeostasis in budding yeast based on high-dimensional chemical-genetic interaction profiles. The morphological responses of 62 Ca2+-sensitive (cls) mutants were quantitatively analyzed with the image processing program CalMorph after exposure to a high concentration of Ca2+. After a generalized linear model was applied, an analysis of covariance model was used to detect significant Ca2+–cls interactions. We found that high-dimensional, morphological Ca2+–cls interactions were mixed with positive (86%) and negative (14%) chemical-genetic interactions, whereas one-dimensional fitness Ca2+–cls interactions were all negative in principle. Clustering analysis with the interaction profiles revealed nine distinct gene groups, six of which were functionally associated. In addition, characterization of Ca2+–cls interactions revealed that morphology-based negative interactions are unique signatures of sensitized cellular processes and pathways. Principal component analysis was used to discriminate between suppression and enhancement of the Ca2+-sensitive phenotypes triggered by inactivation of calcineurin, a Ca2+-dependent phosphatase. Finally, similarity of the interaction profiles was used to reveal a connected network among the Ca2+ homeostasis units acting in different cellular compartments. Our analyses of high-dimensional chemical-genetic interaction profiles provide novel insights into the intracellular network of yeast Ca2+ homeostasis. PMID:28566553

  11. Genesis of theory and analysis of practice of applying the analytical procedures in auditing

    OpenAIRE

    Сурніна, К. С.

    2015-01-01

    Determination of concept "Analytical procedures" in an audit by different researchers is investigated in the article, ownvision of necessity of wideuse of analytical procedures in audit is defined. Classification of analytical procedures is presentedtaking into account the specifity of auditing process on the whole

  12. Evaluation of different strategies for quantitative depth profile analysis of Cu/NiCu layers and multilayers via pulsed glow discharge - Time of flight mass spectrometry

    Science.gov (United States)

    Muñiz, Rocío; Lobo, Lara; Németh, Katalin; Péter, László; Pereiro, Rosario

    2017-09-01

    There is still a lack of approaches for quantitative depth-profiling when dealing with glow discharges (GD) coupled to mass spectrometric detection. The purpose of this work is to develop quantification procedures using pulsed GD (PGD) - time of flight mass spectrometry. In particular, research was focused towards the depth profile analysis of Cu/NiCu nanolayers and multilayers electrodeposited on Si wafers. PGDs are characterized by three different regions due to the temporal application of power: prepeak, plateau and afterglow. This last region is the most sensitive and so it is convenient for quantitative analysis of minor components; however, major elements are often saturated, even at 30 W of applied radiofrequency power for these particular samples. For such cases, we have investigated two strategies based on a multimatrix calibration procedure: (i) using the afterglow region for all the sample components except for the major element (Cu) that was analyzed in the plateau, and (ii) using the afterglow region for all the elements measuring the ArCu signal instead of Cu. Seven homogeneous certified reference materials containing Si, Cr, Fe, Co, Ni and Cu have been used for quantification. Quantitative depth profiles obtained with these two strategies for samples containing 3 or 6 multilayers (of a few tens of nanometers each layer) were in agreement with the expected values, both in terms of thickness and composition of the layers.

  13. Long-Term Restoration of Anterior Shoulder Stability: A Retrospective Analysis of Arthroscopic Bankart Repair Versus Open Latarjet Procedure.

    Science.gov (United States)

    Zimmermann, Stefan M; Scheyerer, Max J; Farshad, Mazda; Catanzaro, Sabrina; Rahm, Stefan; Gerber, Christian

    2016-12-07

    Various operative techniques are used for treating recurrent anterior shoulder instability, and good mid-term results have been reported. The purpose of this study was to compare shoulder stability after treatment with the 2 commonly performed procedures, the arthroscopic Bankart soft-tissue repair and the open coracoid transfer according to Latarjet. A comparative, retrospective case-cohort analysis of 360 patients (364 shoulders) who had primary repair for recurrent anterior shoulder instability between 1998 and 2007 was performed. The minimum duration of follow-up was 6 years. Reoperations, overt recurrent instability (defined as recurrent dislocation or subluxation), apprehension, the subjective shoulder value (SSV), sports participation, and overall satisfaction were recorded. An open Latarjet procedure was performed in 93 shoulders, and an arthroscopic Bankart repair was done in 271 shoulders. Instability or apprehension persisted or recurred after 11% (10) of the 93 Latarjet procedures and after 41.7% (113) of the 271 arthroscopic Bankart procedures. Overt instability recurred after 3% of the Latarjet procedures and after 28.4% (77) of the Bankart procedures. In the Latarjet group, 3.2% of the patients were not satisfied with their result compared with 13.2% in the Bankart group (p = 0.007). Kaplan-Meier analysis of survivorship, with apprehension (p Latarjet procedure and the decreasing effectiveness of the arthroscopic Bankart repair over time. Twenty percent of the first recurrences after arthroscopic Bankart occurred no earlier than 91 months postoperatively, as opposed to the rare recurrences after osseous reconstruction, which occurred in the early postoperative period, with only rare late failures. In this retrospective cohort study, the arthroscopic Bankart procedure was inferior to the open Latarjet procedure for repair of recurrent anterior shoulder dislocation. The difference between the 2 procedures with respect to the quality of outcomes

  14. A Latent Profile Analysis of Latino Parenting: The Infusion of Cultural Values on Family Conflict.

    Science.gov (United States)

    Ayón, Cecilia; Williams, Lela Rankin; Marsiglia, Flavio F; Ayers, Stephanie; Kiehne, Elizabeth

    The purpose of the present study was to (a) examine how acculturation and social support inform Latinos' parenting behaviors, controlling for gender and education; (b) describe parenting styles among Latino immigrants while accounting for cultural elements; and (c) test how these parenting styles are associated with family conflict. A 3 step latent profile analysis with the sample (N = 489) revealed best fit with a 4 profile model (n = 410) of parenting: family parenting (n = 268, 65%), child-centered parenting (n = 68, 17%), moderate parenting (n = 60, 15%), and disciplinarian parenting (n = 14, 3%). Parents' gender, acculturation, and social support significantly predicted profile membership. Disciplinarian and moderate parenting were associated with more family conflict. Recommendations include integrating culturally based parenting practices as a critical element to family interventions to minimize conflict and promote positive youth development.

  15. A Latent Profile Analysis of Latino Parenting: The Infusion of Cultural Values on Family Conflict

    Science.gov (United States)

    Ayón, Cecilia; Williams, Lela Rankin; Marsiglia, Flavio F.; Ayers, Stephanie; Kiehne, Elizabeth

    2015-01-01

    The purpose of the present study was to (a) examine how acculturation and social support inform Latinos’ parenting behaviors, controlling for gender and education; (b) describe parenting styles among Latino immigrants while accounting for cultural elements; and (c) test how these parenting styles are associated with family conflict. A 3 step latent profile analysis with the sample (N = 489) revealed best fit with a 4 profile model (n = 410) of parenting: family parenting (n = 268, 65%), child-centered parenting (n = 68, 17%), moderate parenting (n = 60, 15%), and disciplinarian parenting (n = 14, 3%). Parents’ gender, acculturation, and social support significantly predicted profile membership. Disciplinarian and moderate parenting were associated with more family conflict. Recommendations include integrating culturally based parenting practices as a critical element to family interventions to minimize conflict and promote positive youth development. PMID:26966343

  16. Improved structure, function and compatibility for CellProfiler: modular high-throughput image analysis software.

    Science.gov (United States)

    Kamentsky, Lee; Jones, Thouis R; Fraser, Adam; Bray, Mark-Anthony; Logan, David J; Madden, Katherine L; Ljosa, Vebjorn; Rueden, Curtis; Eliceiri, Kevin W; Carpenter, Anne E

    2011-04-15

    There is a strong and growing need in the biology research community for accurate, automated image analysis. Here, we describe CellProfiler 2.0, which has been engineered to meet the needs of its growing user base. It is more robust and user friendly, with new algorithms and features to facilitate high-throughput work. ImageJ plugins can now be run within a CellProfiler pipeline. CellProfiler 2.0 is free and open source, available at http://www.cellprofiler.org under the GPL v. 2 license. It is available as a packaged application for Macintosh OS X and Microsoft Windows and can be compiled for Linux. anne@broadinstitute.org Supplementary data are available at Bioinformatics online.

  17. Applicability analysis of hexahedral hollow profiles as component elements of supporting systems for gondola cars

    Directory of Open Access Journals (Sweden)

    O. V. Fomin

    2014-12-01

    Full Text Available Purpose. The purpose of work is presentation of features and results of the conducted works on determination of introduction expedience of hexahedral hollow profiles as the component elements of the modern supporting systems of railway freight gondola cars. Methodology. During the research an introduction methodology of different types of profiles as alternative to the existent supporting elements of the body module for freight car was used. This methodology had been developed by the author before. It is oriented to the reduction in material consumption and providing of strength requirements and operating reliability of the car design under study. The developed methodology includes the procedures of admissible values calculation of the resistance moments of the section of the hexahedral hollow profile, which is being introduced. It also includes the determination of optimum (i.e. characterized by the minimum material consumption when meeting the durability requirements values of height and minimum thickness of profile in the conditions of construction limitations. At the same time the admissible resistance moments are calculated as such, which are equal to the value of existent implementation of supporting element or as such that are determined taking into account the surplus design reserve. The first direction is applied in this work. Findings. As a result of the conducted research the introduction expedience of hexahedral hollow profiles as vertical rods of the lateral and latitude belts of the walls of the butt-end freight gondola cars is grounded and the optimum parameters of such replacements are determined. Originality. The problem of the use expedience of hexahedral hollow profiles as the supporting elements of the freight gondola cars bodies was first considered in the article. To solve this problem the mathematical models describing the dependence of basic strength and mass indexes of the proper profiles on varying the geometrical

  18. Multivariate analysis of the scattering profiles of healthy and pathological human breast tissues

    Energy Technology Data Exchange (ETDEWEB)

    Conceicao, A.L.C.; Antoniassi, M. [Departamento de Fisica e Matematica, FFCLRP, Universidade de Sao Paulo, Ribeirao Preto 14040-901, Sao Paulo (Brazil); Cunha, D.M. [Instituto de Fisica, Universidade Federal de Uberlandia, 38400-902, Uberlandia, Minas Gerais (Brazil); Ribeiro-Silva, A. [Departamento de Patologia, HCFMRP, Universidade de Sao Paulo, Ribeirao Preto 14040-901, Sao Paulo (Brazil); Poletti, M.E., E-mail: poletti@ffclrp.usp.br [Departamento de Fisica e Matematica, FFCLRP, Universidade de Sao Paulo, Ribeirao Preto 14040-901, Sao Paulo (Brazil)

    2011-10-01

    Scattering profiles of 106 healthy and pathological human breast samples were obtained using the angular dispersive X-ray scattering technique (AD-XRD) and synchrotron radiation covering the momentum transfer interval of 0.7 nm{sup -1}{<=}q(=4{pi} sin({theta}/2)/{lambda}){<=}70.5 nm{sup -1}. Multivariate analysis in the form of discriminant analysis was applied over the whole scattering profile curve of each sample in order to build a model for breast tissue classification. The classification results were validated and compared with histological sample classification obtained by microscopy analysis. Finally, the model allows classifying correctly 91.5% of the samples and presented values of 98.5%, 89.7% and 0.90 for sensitivity, specificity and Cohen's {kappa}, respectively, in correctly differentiating between healthy and pathological tissues.

  19. Processing of chromatographic data for chemometric analysis of peptide profiles from cheese extracts: a novel approach.

    Science.gov (United States)

    Piraino, Paolo; Parente, Eugenio; McSweeney, Paul L H

    2004-11-17

    Chemometric analysis of chromatograms plays a fundamental role in characterization of foods or in detection of adulteration. Data for multivariate analysis of chromatographic profiles are usually obtained by visual matching (VM) of peaks, the identities of which, as for peptide profiles from cheese extracts, are often unknown. To avoid the main disadvantages of VM, which is subjective and time-consuming, a novel approach was developed. Fuzzy logic was employed to handle in a systematic way uncertainty in the position of peptide peaks, and chromatograms were processed by a rule-based membership function. Processed data consisted of classes of retention time wherein peak heights were accumulated by using the distance from the center of the class as a weight. The novel approach (fuzzy approach, FA) was compared with VM by using a real data set and by performing multivariate descriptive statistical techniques (principal component analysis, multidimensional scaling, and nonhierarchical cluster analysis). FA provided a fast, reliable, and objective alternative to VM and could be successfully applied for chemometric analysis of chromatographic profiles whenever knowledge of the identity of peaks is lacking or unnecessary.

  20. Epidemiologic Analysis of Elective Operative Procedures in Infants Less Than 6 Months of Age in the United States.

    Science.gov (United States)

    Einhorn, Lisa M; Young, Brian J; Routh, Jonathan C; Allori, Alexander C; Tracy, Elisabeth T; Greene, Nathaniel H

    2017-11-01

    This study uses publicly available data to analyze the total number of elective, potentially deferrable operative procedures involving infants Ambulatory Surgery Database was used to identify patients in California, North Carolina, New York, and Utah during the years of 2007-2010 who were younger than 6 months of age at the time that they underwent outpatient (ambulatory) surgery. Operations that could reasonably be postponed until 6 months of age were classified as potentially deferrable procedures. Hernia repairs were analyzed separately from other deferrable procedures. Primary outcomes included the total number of elective procedures and the number and rates of potentially deferrable procedures per state per year in this population. Over the study period, a total of 27,540 procedures were identified as meeting inclusion criteria; of those, 7832 (28%) were classified as potentially deferrable, 4315 of which were hernia repairs. The average rates of potentially deferrable nonhernia procedures in California, North Carolina, New York, and Utah were 8.3, 43.8, 30.0, and 11.7 per 10,000 person-years, respectively. In multivariable analysis, private insurance (odds ratio [OR] = 1.36), self-pay status (OR = 1.50), and treatment in a different state (OR = 0.48-3.16) were independent predictors of a potentially deferrable procedure being performed on an infant younger than 6 months. Potentially deferrable procedures are still performed in infants <6 months of age. There appears to be significant variation in timing of these procedures among states. Insurance status and geography may be independent predictors of a procedure being potentially deferrable.

  1. How school climate relates to chronic absence: A multi-level latent profile analysis.

    Science.gov (United States)

    Van Eck, Kathryn; Johnson, Stacy R; Bettencourt, Amie; Johnson, Sarah Lindstrom

    2017-04-01

    Chronic absence is a significant problem in schools. School climate may play an important role in influencing chronic absence rates among schools, yet little research has evaluated how school climate constructs relate to chronic absence. Using multilevel latent profile analysis, we evaluated how profiles of student perceptions of school climate at both the student and school level differentiated school-level rates of chronic absence. Participants included 25,776 middle and high school students from 106 schools who completed a district administered school climate survey. Students attended schools in a large urban school district where 89% of 6th through 12th grade students were African-American and 61% were eligible for the federally subsidized school meals program. Three student-level profiles of perceptions of school climate emerged that corresponded to "positive," "moderate," and "negative" climate. Two predominant patterns regarding the distribution of these profiles within schools emerged that corresponded to the two school-level profiles of "marginal climate" and "climate challenged" schools. Students reporting "moderate" and "negative" climate in their schools were more likely to attend schools with higher chronic absence rates than students reporting that their school had "positive" climate. Likewise, "climate challenged" schools had significantly higher chronic absence rates than "marginal climate" schools. These results suggest that school climate shares an important relation with chronic absence among adolescent students attending urban schools. Implications for prevention and intervention programs are discussed. Copyright © 2016 Society for the Study of School Psychology. Published by Elsevier Ltd. All rights reserved.

  2. A Latent Profile Analysis of drinking motives among heavy drinking college students.

    Science.gov (United States)

    Cadigan, Jennifer M; Martens, Matthew P; Herman, Keith C

    2015-12-01

    Positively (Enhancement and Social) and negatively (Coping) reinforcing drinking motives have been shown to be associated with alcohol use, alcohol-related problems, and depression among college students. Although prior studies of drinking motives have mostly consisted of variable-centered analyses, the current study used a person-centered approach where individuals were grouped into categories based on shared characteristics using Latent Profile Analysis (LPA). We investigated the utility of drinking motive profiles to determine how different profiles were associated with alcohol outcomes and depressive symptoms. Participants were 648 undergraduate students who had violated a university alcohol policy and who endorsed consuming alcohol in the past month. Social, Coping, and Enhancement subscales from the Drinking Motives Questionnaire were used as indicators. After examining one-through-eight class LPA solutions, the six-class solution provided the best empirical and clinically meaningful fit to the data. Classes with high coping and high positive reinforcing drinking motives consumed more alcohol than profiles of students with high coping and low positive reinforcing motives. Classes high on both coping and positively reinforcing motives reported the most alcohol related problems. Classes with higher levels of coping motives and either high or low positive reinforcing motives reported the highest depression symptoms. Drinking motive profiles differ in terms of alcohol outcomes and depressive symptoms. We encourage researchers to explore motives for drinking with individuals, especially assessing the relationship between coping motives and depression in the presence or absence of positively reinforcing motives. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. A simplified procedure for semi-targeted lipidomic analysis of oxidized phosphatidylcholines induced by UVA irradiation

    Science.gov (United States)

    Gruber, Florian; Bicker, Wolfgang; Oskolkova, Olga V.; Tschachler, Erwin; Bochkov, Valery N.

    2012-01-01

    Oxidized phospholipids (OxPLs) are increasingly recognized as signaling mediators that are not only markers of oxidative stress but are also “makers” of pathology relevant to disease pathogenesis. Understanding the biological role of individual molecular species of OxPLs requires the knowledge of their concentration kinetics in cells and tissues. In this work, we describe a straightforward “fingerprinting” procedure for analysis of a broad spectrum of molecular species generated by oxidation of the four most abundant species of polyunsaturated phosphatidylcholines (OxPCs). The approach is based on liquid-liquid extraction followed by reversed-phase HPLC coupled to electrospray ionization MS/MS. More than 500 peaks corresponding in retention properties to polar and oxidized PCs were detected within 8 min at 99 m/z precursor values using a single diagnostic product ion in extracts from human dermal fibroblasts. Two hundred seventeen of these peaks were fluence-dependently and statistically significantly increased upon exposure of cells to UVA irradiation, suggesting that these are genuine oxidized or oxidatively fragmented species. This method of semitargeted lipidomic analysis may serve as a simple first step for characterization of specific “signatures” of OxPCs produced by different types of oxidative stress in order to select the most informative peaks for identification of their molecular structure and biological role. PMID:22414483

  4. A statistical procedure for the analysis of microbial communities based on phenotypic properties of isolates.

    Science.gov (United States)

    Parente, Eugenio; Ricciardi, Annamaria

    2002-04-01

    A novel statistical procedure for the analysis of microbial communities based on phenotypic properties of randomly collected isolates is presented and discussed. The procedure allows the representation of the microbial communities as a set of ellipses in a bidimensional graph. This representation is obtained by the following steps: (a) measurement of a set of binary phenotypic properties for n isolates belonging to k samples, each representing a different community; (b) repeated sampling by bootstrapping of the m samples, thus obtaining, for each community, i subsamples of j isolates; (c) calculation of the frequency of positive results for each test for each subsample; (d) calculation of the matrix of Euclidean distances between the k x i frequency vectors; (e) use of multidimensional scaling (MDS) to obtain a representation in two dimensions of the distance relationships between the frequency vectors; (f) plotting of the 95% confidence ellipses for the i frequency vectors of each of the k communities. By using both simple, synthetic microbial communities, and samples of lactic acid bacteria isolated from natural microbial communities (sourdoughs, compressed yeast, fermented sausages), it was demonstrated that the position and shape of the ellipses are clearly related to the composition of the community, while the relationship between the size of the ellipses and the phenotypical diversity of the community is less straightforward: while communities with very different diversity (measured with the Functional Evenness index and the mean taxonomic distance) had ellipses that were very different in size, there was no strict proportionality between the size of the ellipse and the diversity of the community. Nevertheless, the representation of microbial communities obtained by bootstrapping and multidimensional scaling appears to be superior to the more usual representation based on tabulation of the frequencies of isolates belonging to different clusters.

  5. Meta-analysis of functional outcomes and complications following transurethral procedures for lower urinary tract symptoms resulting from benign prostatic enlargement.

    Science.gov (United States)

    Ahyai, Sascha A; Gilling, Peter; Kaplan, Steven A; Kuntz, Rainer M; Madersbacher, Stephan; Montorsi, Francesco; Speakman, Mark J; Stief, Christian G

    2010-09-01

    There is a continuous decline in the number of transurethral resections of the prostate (TURP) and an increase use of minimally invasive surgical therapy (MIST) for lower urinary tract symptoms resulting from benign prostatic enlargement. Current results from randomised controlled trials (RCT) and methodologically sound prospective studies suggest that some of the proposed procedures have the potential to replace TURP. To determine the contemporary status of TURP and of the currently most commonly applied transurethral MISTs: (1) bipolar TURP, (2) bipolar transurethral vaporisation of the prostate (bipolar TUVP), (3) holmium laser enucleation of the prostate (HoLEP), and (4) potassium-titanyl-phosphate (KTP) laser vaporisation of the prostate. This meta-analysis was based on a systematic Medline search assessing the period 1997-2009. All RCTs comparing TURP and the most commonly discussed ablative treatments were included. The end points of our analyses were functional outcomes and treatment-related adverse events. Twenty-seven publications involving 23 different RCTs with a total of 2245 patients provided the highest level of evidence available (level 1b) and were fully assessed. Meta-analysis was conducted with SAS v.9.1.3 (SAS Institute, Cary, NC, USA). Forest plots were produced using the R software. Pooled odds ratios and 95% confidence intervals were calculated between various operative techniques versus TURP. Functional results between the specific transurethral procedures versus TURP were summarised as differences in means. This meta-analysis demonstrates statistically comparable efficacy and overall morbidity for MISTs versus contemporary TURP. Type, category (minor vs major), and the number of complications (safety profile) vary specifically for each of the different transurethral techniques. We feel that the individual patient's clinical profile should be carefully assessed to identify the most appropriate transurethral technique. (c) 2010 European

  6. Obsessions and compulsions in the lab: A meta-analysis of procedures to induce symptoms of obsessive-compulsive disorder.

    Science.gov (United States)

    De Putter, Laura M S; Van Yper, Lotte; Koster, Ernst H W

    2017-03-01

    Efficacious induction procedures of symptoms of obsessive-compulsive disorder (OCD) are necessary in order to test central tenets of theories on OCD. However, the efficacy of the current range of induction procedures remains unclear. Therefore, this meta-analysis set out to examine the efficacy of induction procedures in participants with and without OCD symptoms. Moreover, we explored whether the efficacy varied across different moderators (i.e., induction categories, symptom dimensions of OCD, modalities of presentation, and level of individual tailoring). In total we included 4900 participants across 90 studies. The analyses showed that there was no difference in studies using subclinical and clinical participants, confirming the utility of analogue samples. Induction procedures evoked more symptoms in (sub)clinical OCD than in healthy participants, which was most evident in the contamination symptom dimension of OCD. Analysis within (sub)clinical OCD showed a large effect size of induction procedures, especially for the threat and responsibility category and when stimuli were tailored to individuals. Analysis within healthy participants showed a medium effect size of induction procedures. The magnitude of the effect in healthy individuals was stronger for mental contamination, thought-action fusion and threat inductions. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Numerical Analysis of Composite Steel Concrete Structural Shear Walls with Steel Encased Profiles

    Directory of Open Access Journals (Sweden)

    Daniel Dan

    2009-01-01

    Full Text Available The use of common reinforced concrete shear walls in high rise buildings is sometimes limited because of the large amount of reinforcement localized at the end of the element. A good alternative in avoiding this disadvantage is to use composite steel concrete structural shear walls with steel encased profiles. This solution used for high rise buildings, offers to designers lateral stiffness, shear capacity and high bending resisting moment of structural walls. The encasement of the steel shapes in concrete is applied also for the following purposes: flexural stiffening and strengthening of compression elements; fire protection; potentially easier repairs after moderate damage; economy with respect both to material and construction. Until now in the national and international literature poor information about nonlinear behaviour of composite steel concrete structural shear walls with steel encased profiles is available. A theoretical and experimental program related to the behaviour of steel concrete structural shear walls with steel encased profiles is developed at “Politehnica” University of Timişoara. The program refers to six different elements, which differ by the shape of the steel encased profile and also by the arrangement of steel shapes on the cross section of the element. In order to calibrate the elements for experimental study some numerical analysis were made. The paper presents the results of numerical analysis with details of stress distribution, crack distribution, structural stiffness at various loads, and load bearing capacity of the elements.

  8. Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling.

    Directory of Open Access Journals (Sweden)

    Hans J C T Wessels

    Full Text Available Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels. Here we present a dataset of blue native electrophoresis migration profiles for 953 proteins by complexome profiling. By analysis of mitochondrial ribosomal complexes we demonstrate its potential to verify putative protein-protein interactions identified by affinity purification-mass spectrometry studies. Protein complexes were extracted in their native state from a HEK293 mitochondrial fraction and separated by blue native gel electrophoresis. Gel lanes were cut into gel slices of even size and analyzed by shotgun proteomics. Subsequently, the acquired protein migration profiles were analyzed for co-migration via hierarchical cluster analysis. This dataset holds great promise as a comprehensive resource for de novo identification of protein-protein interactions or to underpin and prioritize candidate protein interactions from other studies. To demonstrate the potential use of our dataset we focussed on the mitochondrial translation machinery. Our results show that mitoribosomal complexes can be analyzed by blue native gel electrophoresis, as at least four distinct complexes. Analysis of these complexes confirmed that 24 proteins that had previously been reported to co-purify with mitoribosomes indeed co-migrated with subunits of the mitochondrial ribosome. Co-migration of several proteins involved in biogenesis of inner mitochondrial membrane complexes together with mitoribosomal complexes suggested the possibility of co-translational assembly in human cells. Our data also highlighted a putative ribonucleotide complex that potentially contains MRPL10, MRPL12 and MRPL53 together with LRPPRC and SLIRP.

  9. LCMS analysis of fingerprints, the amino acid profile of 20 donors.

    Science.gov (United States)

    de Puit, Marcel; Ismail, Mahado; Xu, Xiaoma

    2014-03-01

    The analysis of amino acids present in fingerprints has been studied several times. In this paper, we report a method for the analysis of amino acids using an fluorenylmethyloxycarbonyl chloride-derivatization for LC separation and MS detection. We have obtained good results with regard to the calibration curves and the limit of detection and LOQ for the target compounds. The extraction of the amino acids from the substrates used proved to be very efficient. Analysis of the derivatized amino acids enabled us to obtain full amino acid profiles for 20 donors. The intervariability is as expected rather large, with serine as the most abundant constituent, and when examining the total profile of the amino acids per donor, a characteristic pattern can be observed. Some amino acids were not detected in some donors, or fell out of the range of the calibration curve, where others showed a surprisingly high amount of material in the deposition analyses. Further investigations will have to address the intravariability of the amino acid profiles of the fingerprints from donors. By the development of the analytical method and the application to the analysis of fingerprints, we were able to gain insight in the variability of the constituents of fingerprints between the donors. © 2013 American Academy of Forensic Sciences.

  10. Analysis of "never events" following adult cardiac surgical procedures in the United States.

    Science.gov (United States)

    Robich, Michael P; Krafcik, Brianna M; Shah, Nishant K; Farber, Alik; Rybin, Denis; Siracuse, Jeffrey J

    2017-10-01

    This study was conducted to determine the risk factors, nature, and outcomes of "never events" following open adult cardiac surgical procedures. Understanding of these events can reduce their occurrence, and thereby improve patient care, quality metrics, and cost reduction. "Never events" for patients included in the Nationwide Inpatient Sample who underwent coronary artery bypass graft, heart valve repair/replacement, or thoracic aneurysm repair between 2003-2011 were documented. These events included air embolism, catheter-based urinary tract infection (UTI), pressure ulcer, falls/trauma, blood incompatibility, vascular catheter infection, poor glucose control, foreign object retention, wrong site surgery and mediastinitis. Analysis included characterization of preoperative demographics, comorbidities and outcomes for patients sustaining never events, and multivariate analysis of predictive risk factors and outcomes. A total of 588,417 patients meeting inclusion criteria were identified. Of these, never events occurred in 4377 cases. The majority of events were in-hospital falls, vascular catheter infections, and complications of poor glucose control. Rates of falls, catheter based UTIs, and glucose control complications increased between 2009-2011 as compared to 2003-2008. Analysis revealed increased hospital length of stay, hospital charges, and mortality in patients who suffered a never event as compared to those that did not. This study establishes a baseline never event rate after cardiac surgery. Adverse patient outcomes and increased resource utilization resulting from never events emphasizes the need for quality improvement surrounding them. A better understanding of individual patient characteristics for those at risk can help in developing protocols to decrease occurrence rates.

  11. Role of laser irradiation in direct pulp capping procedures: a systematic review and meta-analysis.

    Science.gov (United States)

    Javed, Fawad; Kellesarian, Sergio Varela; Abduljabbar, Tariq; Gholamiazizi, Elham; Feng, Changyong; Aldosary, Khaled; Vohra, Fahim; Romanos, Georgios E

    2017-02-01

    A variety of materials are available to treat exposed dental pulp by direct pulp capping. The healing response of the pulp is crucial to form a dentin bridge and seal off the exposed pulp. Studies have used lasers to stimulate the exposed pulp to form tertiary dentin. The aim of the present systematic review and meta-analysis was to evaluate the evidence on the effects of laser irradiation as an adjunctive therapy to stimulate healing after pulp exposure. A systematic literature search was conducted up to April 2016. A structured search using the keywords "Direct pulp capping," "Lasers," "Calcium hydroxide pulp capping," and "Resin pulp capping" was performed. Initially, 34 potentially relevant articles were identified. After removal of duplicates and screening by title, abstract, and full text when necessary, nine studies were included. Studies were assessed for bias and data were synthetized using a random-effects meta-analysis model. Six studies were clinical, and three were preclinical animal trials; the follow-up period ranged from 2 weeks to 54 months. More than two thirds of the included studies showed that laser therapy used as an adjunct for direct pulp capping was more effective in maintaining pulp vitality than conventional therapy alone. Meta-analysis showed that the success rate in the laser treatment group was significantly higher than the control group (log odds ratio = 1.737; 95 % confidence interval, 1.304-2.171). Lasers treatment of exposed pulps can improve the outcome of direct pulp capping procedures; a number of confounding factors may have influenced the outcomes of the included studies.

  12. Homogenization procedures for the constitutive material modeling and analysis of aperiodic micro-structures

    Science.gov (United States)

    Aghalaya Manjunatha, Preetham

    Composite materials are the well-known substitutes for traditional metals in various industries because of their micro-structural character. Micro-structures provide a high strength-to-weight ratio, which makes them suitable for manufacturing large variety of applications ranging from simple toys to complicated space/aircraft structures. Since, these materials are widely used in high performance structures, their stress/thermal analysis issues are of major concern. Due to the high degree of material heterogeneity, it is extremely difficult to analyze such structures. Homogenization (rigorous averaging) is a process that overcomes the difficulty of modeling each micro-structure. It replaces an individual micro-structure by an equivalent material model representation (unit cell). Periodic micro-structures appear in regular intervals throughout the domain, in contrast aperiodic micro-structures follows an irregular pattern. Further, this method bridges the analysis gap between micro and macro domain of the structures. In this thesis, Homogenization procedure based on anti-periodic displacement fields for aperiodic micro-structures and aperiodic boundary conditions are considered to model the constitutive material matrix. This work could be easily implemented with the traditional finite element packages. In addition, it eventually increases the convergence accuracy and reduces the high computational expenses. Different problems are analyzed by the implementation of digital image processing schemes for the extraction of a unit cell around the Gauss quadrature points and the mesh-generation. In the future, this research defines a new path for the analysis of any random heterogeneous materials by its ease of implementation and the state-of-the-art micro-structure material modeling capabilities and digital image based micro-meshing.

  13. An analysis of marketing authorisation applications via the mutual recognition and decentralised procedures in Europe

    DEFF Research Database (Denmark)

    Ebbers, Hans C; Langedijk, Joris; Bouvy, Jacoline C

    2015-01-01

    PURPOSE: The aim of this study is to provide a comprehensive overview of the outcomes of marketing authorisation applications via the mutual recognition and decentralised procedures (MRP/DCP) and assess determinants of licensing failure during CMDh referral procedures. METHODS: All MRP...... the frequency of licensing failure prior to CMDh referrals. RESULTS: During the study period, 10392 MRP/DCP procedures were finalized. Three hundred seventy-seven (3.6%) resulted in a referral procedure, of which 70 (19%) resulted in licensing failure, defined as refusal or withdrawal of the application...... program. Study design issues and objections about the demonstration of equivalence were most likely to result in licensing failure. An estimated 11% of all MRP/DCP procedures resulted in licensing failure prior to CMDh referral. CONCLUSION: Whereas the absolute number of MRP/DCP procedures resulting...

  14. Evaluation of some procedures relevant to the determination of trace elemental components in biological materials by destructive neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Berry, D.L.

    1979-01-01

    The development of a simplified procedure for the analysis of biological materials by destructive neutron activation analysis (DNAA) is described. The sample manipulations preceding gamma ray assay were investigated as five specific stages of processing: (1) pre-irradiation treatment; (2) sample irradiation; (3) removal of the organic matrix; (4) removal of interfering radioactivities; and (5) concentration and separation of analyte activities. Each stage was evaluated with respect to susceptibility to sample contamination, loss of trace elemental components, and compatibility with other operations in the overall DNAA procedures. A complete DNAA procedure was proposed and evaluated for the analysis of standard bovine liver and blood samples. The DNAA system was effective for the determination of As, Cu, Fe, Hg, Mo, Rb, Sb, Se, and Zn without yield determinations and with a minimum turn-around time of approximately 3 days.

  15. Analysis of Motivational Profiles of Satisfaction and Importance of Physical Education in High School Adolescents

    Science.gov (United States)

    Granero-Gallegos, Antonio; Baena-Extremera, Antonio; Pérez-Quero, Francisco J.; Ortiz-Camacho, Maria M.; Bracho-Amador, Clara

    2012-01-01

    The purpose of this study was to analyze the motivational profiles of satisfaction with and importance of physical education in high school students and its relation with gender and the practice of sport. The sample comprised 2002 students aged from 12 to 19 who completed the Sport Motivation Scale (Núñez et al., 2006), the Sport Satisfaction Instrument (Baena-Extremera et al., 2012) and the Importance of Physical Education Scale (Moreno et al., 2009). Descriptive analyzes, correlations between the scales, a cluster analysis for profiles, and a MANOVA were conducted to examine differences by gender. Three clusters (profiles) were identified. The first profile identified was "moderate" motivation (n = 463) and was associated with boys who practice physical activity for less than 3 hours per week. The second profile identified was "low" motivation (n = 545) and was associated mainly with girls who practice physical activity for less than 3 hours per week. And lastly the third profile identified was "high" motivation (n = 910), which was found to be greater in boys who practiced physical exercise for more than 3 hours a week. Key points High school students’ motivation was mainly intrinsic, scoring very low on a motivation and high on satisfaction/fun; equally, these students were task-oriented. There appears to be a positive relationship between intrinsic motivation and task-orientation in high school students. The subject of PE is considered very highly by the students, and hence it obtains high values on satisfaction/fun and is positively associated with the importance given to PE. PMID:24150070

  16. The national psychological/personality profile of Romanians: An in depth analysis of the regional national psychological/personality profile of Romanians

    Directory of Open Access Journals (Sweden)

    David, D.

    2015-12-01

    Full Text Available In this article we perform an in depth analysis of the national psychological/personality profile of Romanians. Following recent developments in the field (see Rentfrow et al., 2013; 2015, we study the regional national psychological/personality profile of Romanians, based on the Big Five model (i.e., NEO PI/R. Using a representative sample (N1 = 1000, we performed a cluster analysis and identified two bipolar personality profiles in the population: cluster 1, called “Factor X-”, characterized by high neuroticism and low levels of extraversion, openness, agreeableness, and conscientiousness, and cluster 2, called “Factor X+”, characterized by the opposite configuration in personality traits, low neuroticism and high levels of extraversion, openness, agreeableness, and conscientiousness. The same two cluster pattern/solution emerged in other samples (N = 2200, with other Big Five-based instruments, and by using various methods of data (e.g., direct vs. reversed item score, controlling for item desirability and cluster (i.e., with and without “running means” analyses. These two profiles are quite evenly distributed in the overall population, but also across all geographical regions. Moreover, comparing the distribution of the five personality traits, we found just few small differences between the eight geographical divisions that we used for our analysis. These results suggest that the regional national psychological/personality profile of Romania is quite homogenous. Directions for harnessing the potential of both personality profiles are presented to the reader. Other implications based on the bipolar and fractal structure of the personality profile are discussed from an interdisciplinary perspective.

  17. Multivariate Analysis Approach to the Serum Peptide Profile of Morbidly Obese Patients

    Directory of Open Access Journals (Sweden)

    M. Agostini

    2013-01-01

    Full Text Available Background: Obesity is currently epidemic in many countries worldwide and is strongly related to diabetes and cardiovascular disease. Mass spectrometry, in particular matrix-assisted laser desorption/ionization time of flight (MALDI-TOF is currently used for detecting different pattern of expressed protein. This study investigated the differences in low molecular weight (LMW peptide profiles between obese and normal-weight subjects in combination with multivariate statistical analysis.

  18. CellProfiler Analyst: data exploration and analysis software for complex image-based screens.

    Science.gov (United States)

    Jones, Thouis R; Kang, In Han; Wheeler, Douglas B; Lindquist, Robert A; Papallo, Adam; Sabatini, David M; Golland, Polina; Carpenter, Anne E

    2008-11-15

    Image-based screens can produce hundreds of measured features for each of hundreds of millions of individual cells in a single experiment. Here, we describe CellProfiler Analyst, open-source software for the interactive exploration and analysis of multidimensional data, particularly data from high-throughput, image-based experiments. The system enables interactive data exploration for image-based screens and automated scoring of complex phenotypes that require combinations of multiple measured features per cell.

  19. A simplified calculation procedure for mass isotopomer distribution analysis (MIDA) based on multiple linear regression.

    Science.gov (United States)

    Fernández-Fernández, Mario; Rodríguez-González, Pablo; García Alonso, J Ignacio

    2016-10-01

    We have developed a novel, rapid and easy calculation procedure for Mass Isotopomer Distribution Analysis based on multiple linear regression which allows the simultaneous calculation of the precursor pool enrichment and the fraction of newly synthesized labelled proteins (fractional synthesis) using linear algebra. To test this approach, we used the peptide RGGGLK as a model tryptic peptide containing three subunits of glycine. We selected glycine labelled in two 13 C atoms ( 13 C 2 -glycine) as labelled amino acid to demonstrate that spectral overlap is not a problem in the proposed methodology. The developed methodology was tested first in vitro by changing the precursor pool enrichment from 10 to 40% of 13 C 2 -glycine. Secondly, a simulated in vivo synthesis of proteins was designed by combining the natural abundance RGGGLK peptide and 10 or 20% 13 C 2 -glycine at 1 : 1, 1 : 3 and 3 : 1 ratios. Precursor pool enrichments and fractional synthesis values were calculated with satisfactory precision and accuracy using a simple spreadsheet. This novel approach can provide a relatively rapid and easy means to measure protein turnover based on stable isotope tracers. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  20. Modular approach to customise sample preparation procedures for viral metagenomics: a reproducible protocol for virome analysis.

    Science.gov (United States)

    Conceição-Neto, Nádia; Zeller, Mark; Lefrère, Hanne; De Bruyn, Pieter; Beller, Leen; Deboutte, Ward; Yinda, Claude Kwe; Lavigne, Rob; Maes, Piet; Van Ranst, Marc; Heylen, Elisabeth; Matthijnssens, Jelle

    2015-11-12

    A major limitation for better understanding the role of the human gut virome in health and disease is the lack of validated methods that allow high throughput virome analysis. To overcome this, we evaluated the quantitative effect of homogenisation, centrifugation, filtration, chloroform treatment and random amplification on a mock-virome (containing nine highly diverse viruses) and a bacterial mock-community (containing four faecal bacterial species) using quantitative PCR and next-generation sequencing. This resulted in an optimised protocol that was able to recover all viruses present in the mock-virome and strongly alters the ratio of viral versus bacterial and 16S rRNA genetic material in favour of viruses (from 43.2% to 96.7% viral reads and from 47.6% to 0.19% bacterial reads). Furthermore, our study indicated that most of the currently used virome protocols, using small filter pores and/or stringent centrifugation conditions may have largely overlooked large viruses present in viromes. We propose NetoVIR (Novel enrichment technique of VIRomes), which allows for a fast, reproducible and high throughput sample preparation for viral metagenomics studies, introducing minimal bias. This procedure is optimised mainly for faecal samples, but with appropriate concentration steps can also be used for other sample types with lower initial viral loads.

  1. Reliable and Efficient Procedure for Steady-State Analysis of Nonautonomous and Autonomous Systems

    Directory of Open Access Journals (Sweden)

    J. Dobes

    2012-04-01

    Full Text Available The majority of contemporary design tools do not still contain steady-state algorithms, especially for the autonomous systems. This is mainly caused by insufficient accuracy of the algorithm for numerical integration, but also by unreliable steady-state algorithms themselves. Therefore, in the paper, a very stable and efficient procedure for the numerical integration of nonlinear differential-algebraic systems is defined first. Afterwards, two improved methods are defined for finding the steady state, which use this integration algorithm in their iteration loops. The first is based on the idea of extrapolation, and the second utilizes nonstandard time-domain sensitivity analysis. The two steady-state algorithms are compared by analyses of a rectifier and a C-class amplifier, and the extrapolation algorithm is primarily selected as a more reliable alternative. Finally, the method based on the extrapolation naturally cooperating with the algorithm for solving the differential-algebraic systems is thoroughly tested on various electronic circuits: Van der Pol and Colpitts oscillators, fragment of a large bipolar logical circuit, feedback and distributed microwave oscillators, and power amplifier. The results confirm that the extrapolation method is faster than a classical plain numerical integration, especially for larger circuits with complicated transients.

  2. Comparative gene expression profile analysis between native human odontoblasts and pulp tissue.

    Science.gov (United States)

    Pääkkönen, V; Vuoristo, J T; Salo, T; Tjäderhane, L

    2008-02-01

    To undertake a large-scale analysis of the expression profiles of native human pulp tissue and odontoblasts, and search for genes expressed only in odontoblasts. Microarray was performed to pooled pulp and odontoblasts of native human third molars and to pooled +/- TGF-beta1 cultured pulps and odontoblasts (137 teeth). The repeatability of microarray analysis was estimated by comparing the experimental pulp samples with expression profiles of two pulp samples downloaded from the GEO database. The genes expressed only in the experimental pulp samples or in odontoblasts were divided into categories, and the expression of selected odontoblast-specific genes of extracellular matrix (ECM) organization and biogenesis category was confirmed with RT-PCR and Western blot. A 85.3% repeatability was observed between pulp microarrays, demonstrating the high reliability of the technique. Overall 1595 probe sets were positive only in pulp and 904 only in odontoblasts. Sixteen expressed sequence tags (ESTs), which represent transcribed sequences encoding possibly unknown genes, were detected only in odontoblasts; two consistently expressed in all odontoblast samples. Matrilin 4 (MATN4) was the only ECM biogenesis and organization related gene detected in odontoblasts but not in pulp by microarray and RT-PCR. MATN4 protein expression only in odontoblasts was confirmed by Western blot. Pulp tissue and odontoblast gene expression profiling provides basic data for further, more detailed protein analysis. In addition, MATN4 and the two ESTs could serve as an odontoblast differentiation marker, e.g. in odontoblast stem cell research.

  3. Profiling of illicit cocaine seized in China by ICP-MS analysis of inorganic elements.

    Science.gov (United States)

    Liu, Cuimei; Hua, Zhendong; Meng, Xin

    2017-07-01

    For the first time in China, the inorganic element profiling of cocaine specimens was performed at the National Narcotics Laboratory. An inductive coupled plasma-mass spectrometry (ICP-MS) method was developed and validated for simultaneous analysis of 26 inorganic elements, including sodium (Na), magnesium (Mg), aluminuim (Al), potassium (K), calcium (Ca), vanadium (V), chromium (Cr), manganese (Mn), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), selenium (Se), molybdenum (Mo), silver (Ag), cadmium (Cd), antimony (Sb), barium (Ba), lanthanum (La), cerium (Ce), thallium (Tl), lead (Pb), thorium (Th), and uranium (U) in illicit cocaine samples. Ultrasonic assisted dissolution was applied for sample preparation. Minimum sample preparation and analysis time were required, which was suitable for routine analysis. After the analysis of 183 cocaine samples seized from 2011 to 2015, the element concentration ranges of cocaine sample were obtained. Based on the quantitative data set of 26 inorganic elements in 131 linked/un-linked cocaine samples, fifty combinations of pre-treatment methods and distance/correlation measurements were tested for their potential discrimination power for cocaine profiling, and normalization+standardization+logarithm (N+S+L)/Cosine correlation exhibited the best result. After hierarchical cluster analysis (HCA) analysis of 183 cocaine samples, 21 groups of linked samples were found within and between provinces, which provide intelligence for case connection and revealing of the distribution networks. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Changes in soft tissue profile following the treatment using a Herbst appliance: A photographic analysis

    Directory of Open Access Journals (Sweden)

    Nedeljković Nenad

    2014-01-01

    Full Text Available Background/Aim. Aesthetics is the reason for most of the class II malocclusion patients to opt for orthodontic treatment. In order to regulate retrognathic profile Herbst appliance for anterior movement of the mandible can be a treatment of choice. The aim of this study was to determine the soft tissue profile changes following Herbst appliance therapy on before and after treatment photos, using the computer program. Methods. This investigation was performed on profile photographs of 20 class II patients (12 females and 8 males aged 18-23 years. Analysis of the changes in soft tissue facial structure relationships evident on the photographs before and after the Herbst appliance therapy was performed using Bentley Micro Station program. The first contour of the soft tissue profile was marked. The following reference lines were subsequently traced: Ricketts aesthetic E line and Juanita line. The area enclosed by these two lines included the nose, upper and lower lip, chin and free space in front of the lips. Using the computer program, the surfaces of the soft tissue structures and free space defined by the reference lines and profile contours were measured. Calculation of the relative proportion of surfaces was done for each photograph. The data obtained were then compared for each patient before and after the treatment. Skeletal and dentoalveolar treatment effects that support soft tissue changes were presented by the profile cephalometric parameters of sagital occlusion (SO analysis. Results. A reduction in the relative surface of the upper lip in males (p < 0.01 and females (p < 0.05 was shown by the pictures. The space occupied by the chin was reduced after the treatment for females only (p < 0.05. The relative surface of the nasal soft tissues, that was included in the reference space was increased (p < 0.01 in both genders. The relationship between the soft tissue and empty surface was changed in favor of the empty surface (p < 0.05 in

  5. Changes in soft tissue profile following the treatment using a Herbst appliance--a photographic analysis.

    Science.gov (United States)

    Nedeljković, Nenad; Cubrilo, Danka; Hadzi-Mihailović, Milos

    2014-01-01

    Aesthetics is the reason for most of the class II malocclusion patients to opt for orthodontic treatment. In order to regulate retrognathic profile Herbst appliance for anterior movement of the mandible can be a treatment of choice. The aim of this study was to determine the soft tissue profile changes following Herbst appliance therapy on before and after treatment photos, using the computer program. This investigation was performed on profile photographs of 20 class II patients (12 females and 8 males) aged 18-23 years. Analysis of the changes in soft tissue facial structure relationships evident on the photographs before and after the Herbst appliance therapy was performed using Bentley Micro Station program. The first contour of the soft tissue profile was marked. The following reference lines were subsequently traced: Ricketts aesthetic E line and Juanita line. The area enclosed by these two lines included the nose, upper and lower lip, chin and free space in front of the lips. Using the computer program, the surfaces of the soft tissue structures and free space defined by the reference lines and profile contours were measured. Calculation of the relative proportion of surfaces was done for each photograph. The data obtained were then compared for each patient before and after the treatment. Skeletal and dentoalveolar treatment effects that support soft tissue changes were presented by the profile cephalometric parameters of sagital occlusion (SO) analysis. A reduction in the relative surface of the upper lip in males (p space occupied by the chin was reduced after the treatment for females only (p space was increased (p < 0.01) in both genders. The relationship between the soft tissue and empty surface was changed in favor of the empty surface (p < 0.05) in females. No statistically significant differences were found between the males and the females, before or after the Herbst appliance therapy. The soft tissue changes were the consequence of skeletal and

  6. A Component Analysis of Toilet-Training Procedures Recommended for Young Children

    Science.gov (United States)

    Greer, Brian D.; Neidert, Pamela L.; Dozier, Claudia L.

    2016-01-01

    We evaluated the combined and sequential effects of 3 toilet-training procedures recommended for use with young children: (a) underwear, (b) a dense sit schedule, and (c) differential reinforcement. A total of 20 children participated. Classroom teachers implemented a toilet-training package consisting of all 3 procedures with 6 children. Of the 6…

  7. Is the Latarjet procedure risky? Analysis of complications and learning curve.

    Science.gov (United States)

    Dauzère, Florence; Faraud, Amélie; Lebon, Julie; Faruch, Marie; Mansat, Pierre; Bonnevialle, Nicolas

    2016-02-01

    The purpose of this study was to analyse the learning curve and complication rate of the open Latarjet procedure. The first 68 Latarjet procedures performed by a single surgeon for chronic anterior shoulder instability were reviewed retrospectively. The standard open surgical technique was followed faithfully during each procedure. Post-operative complications were taken from patient medical records. Post-operative evaluation consisted of clinical and radiological assessments. The rate of early (Latarjet procedure remains low. A surgeon's experience significantly affects the surgery duration and the occurrence of early complications. The main radiological complication is partial lysis of the bone block. After a short learning curve, the clinical outcomes of the Latarjet procedure appear to be satisfactory and reproducible. IV.

  8. Application of a trigonometric finite difference procedure to numerical analysis of compressive and shear buckling of orthotropic panels

    Science.gov (United States)

    Stein, M.; Housner, J. D.

    1978-01-01

    A numerical analysis developed for the buckling of rectangular orthotropic layered panels under combined shear and compression is described. This analysis uses a central finite difference procedure based on trigonometric functions instead of using the conventional finite differences which are based on polynomial functions. Inasmuch as the buckle mode shape is usually trigonometric in nature, the analysis using trigonometric finite differences can be made to exhibit a much faster convergence rate than that using conventional differences. Also, the trigonometric finite difference procedure leads to difference equations having the same form as conventional finite differences; thereby allowing available conventional finite difference formulations to be converted readily to trigonometric form. For two-dimensional problems, the procedure introduces two numerical parameters into the analysis. Engineering approaches for the selection of these parameters are presented and the analysis procedure is demonstrated by application to several isotropic and orthotropic panel buckling problems. Among these problems is the shear buckling of stiffened isotropic and filamentary composite panels in which the stiffener is broken. Results indicate that a break may degrade the effect of the stiffener to the extent that the panel will not carry much more load than if the stiffener were absent.

  9. Narcissistic self-esteem or optimal self-esteem? A Latent Profile Analysis of self-esteem and psychological entitlement

    OpenAIRE

    Stronge, Sam; Cichocka, Aleksandra; Sibley, Chris G.

    2016-01-01

    Research into the relationship between self-esteem and narcissism has produced conflicting results, potentially caused by hidden subpopulations that exhibit distinct positive or negative associations. This research uses Latent Profile Analysis to identify profiles within a national panel study (N = 6,471) with differing relationships between psychological entitlement and self-esteem. We identified a narcissistic self-esteem profile (9%) characterised by high entitlement and high self-esteem, ...

  10. A latent profile analysis of attributions for poverty: Identifying response patterns underlying people's willingness to help the poor

    OpenAIRE

    Osborne, D; Weiner, B.

    2015-01-01

    © 2015 Elsevier Ltd. Attribution theory posits that poverty beliefs vary along the causal properties of locus, stability, personal control, and other control. The current paper extends this framework by using a latent profile analysis to identify the unique response patterns (latent profiles) that underlie these beliefs. Results indicated that participants (N= 315) displayed one of three attributional profiles: Blaming (high internal locus; moderate stability; high personal control; high othe...

  11. An integrated multi-scale risk analysis procedure for pluvial flooding

    Science.gov (United States)

    Tader, Andreas; Mergili, Martin; Jäger, Stefan; Glade, Thomas; Neuhold, Clemens; Stiefelmeyer, Heinz

    2016-04-01

    Mitigation of or adaptation to the negative impacts of natural processes on society requires a better understanding of the spatio-temporal distribution not only of the processes themselves, but also of the elements at risk. Information on their values, exposures and vulnerabilities towards the expected impact magnitudes/intensities of the relevant processes is needed. GIS-supported methods are particularly useful for integrated spatio-temporal analyses of natural processes and their potential consequences. Hereby, pluvial floods are of particular concern for many parts of Austria. The overall aim of the present study is to calculate the hazards emanating from pluvial floods, to determine the exposure of given elements at risk, to determine their vulnerabilities towards given pluvial flood hazards and to analyze potential consequences in terms of monetary losses. The whole approach builds on data available on a national scale. We introduce an integrated, multi-scale risk analysis procedure with regard to pluvial flooding. Focusing on the risk to buildings, we firstly exemplify this procedure with a well-documented event in the city of Graz (Austria), in order to highlight the associated potentials and limitations. Secondly, we attempt to predict the possible consequences of pluvial flooding triggered by rainfall events with recurrence intervals of 30, 100 and 300 years. (i) We compute spatially distributed inundation depths using the software FloodArea. Infiltration capacity and surface roughness are estimated from the land cover units given by the official cadastre. Various assumptions are tested with regard to the inflow to the urban sewer system. (ii) Based on the inundation depths and the official building register, we employ a set of rules and functions to deduce the exposure, vulnerability and risk for each building. A risk indicator for each building, expressed as the expected damage associated to a given event, is derived by combining the building value and

  12. Analysis of Mass Profiles and Cooling Flows of Bright, Early-Type Galaxies AO2, AO3 and Surface Brightness Profiles and Energetics of Intracluster Gas in Cool Galaxy Clusters AO3

    Science.gov (United States)

    White, Raymond E., III

    1998-01-01

    This final report uses ROSAT observations to analyze two different studies. These studies are: Analysis of Mass Profiles and Cooling Flows of Bright, Early-Type Galaxies; and Surface Brightness Profiles and Energetics of Intracluster Gas in Cool Galaxy Clusters.

  13. Tenacious goal pursuit and flexible goal adjustment in older people over 5 years: a latent profile transition analysis.

    Science.gov (United States)

    Bailly, Nathalie; Martinent, Guillaume; Ferrand, Claude; Kamel, Gana; Joulain, Michèle; Maintier, Christine

    2016-03-01

    previous research has shown that tendencies to tenaciously pursue goals (TGP) and flexibly adapt goals (FGA) relate to well-being of older people. this study aimed to identify subpopulations of elderly people with different coping profiles, describe change in participants' profiles over time and determine the influence of coping profiles on well-being over a 5-year period. latent profile transition analysis (LPTA) was used in a three-wave longitudinal data collection measuring flexibility, tenacity, depression, self-rated health and life satisfaction among an elderly population over 65 years old. three coping profiles were identified. Profile 1 was characterised by participants with high flexibility and tenacity scores, Profile 2 with moderate flexibility and low tenacity scores and Profile 3 with low flexibility and moderate tenacity scores. Results indicate stability of these profiles over time, with Profile 1 being the most adaptive in terms of ageing well. high flexibility and tenacity in older people is a stable coping profile that is associated with successful ageing. © The Author 2016. Published by Oxford University Press on behalf of the British Geriatrics Society. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  14. A solid-phase extraction procedure coupled to {sup 1}H NMR, with chemometric analysis, to seek reliable markers of the botanical origin of honey

    Energy Technology Data Exchange (ETDEWEB)

    Beretta, Giangiacomo [Istituto di Chimica Farmaceutica e Tossicologica ' Pietro Pratesi' , Faculty of Pharmacy, University of Milan, via Mangiagalli 25, 20133 Milan (Italy)], E-mail: giangiacomo.beretta@unimi.it; Caneva, Enrico [Ciga - Centro Interdipartimentale Grandi Apparecchiature, University of Milan, via Golgi 19, 20133 Milan (Italy); Regazzoni, Luca; Bakhtyari, Nazanin Golbamaki; Maffei Facino, Roberto [Istituto di Chimica Farmaceutica e Tossicologica ' Pietro Pratesi' , Faculty of Pharmacy, University of Milan, via Mangiagalli 25, 20133 Milan (Italy)

    2008-07-14

    The aim of this work was to establish an analytical method for identifying the botanical origin of honey, as an alternative to conventional melissopalynological, organoleptic and instrumental methods (gas-chromatography coupled to mass spectrometry (GC-MS), high-performance liquid chromatography HPLC). The procedure is based on the {sup 1}H nuclear magnetic resonance (NMR) profile coupled, when necessary, with electrospray ionisation-mass spectrometry (ESI-MS) and two-dimensional NMR analyses of solid-phase extraction (SPE)-purified honey samples, followed by chemometric analyses. Extracts of 44 commercial Italian honeys from 20 different botanical sources were analyzed. Honeydew, chestnut and linden honeys showed constant, specific, well-resolved resonances, suitable for use as markers of origin. Honeydew honey contained the typical resonances of an aliphatic component, very likely deriving from the plant phloem sap or excreted into it by sap-sucking aphids. Chestnut honey contained the typical signals of kynurenic acid and some structurally related metabolite. In linden honey the {sup 1}H NMR profile gave strong signals attributable to the mono-terpene derivative cyclohexa-1,3-diene-1-carboxylic acid (CDCA) and to its 1-O-{beta}-gentiobiosyl ester (CDCA-GBE). These markers were not detectable in the other honeys, except for the less common nectar honey from rosa mosqueta. We compared and analyzed the data by multivariate techniques. Principal component analysis found different clusters of honeys based on the presence of these specific markers. The results, although obviously only preliminary, suggest that the {sup 1}H NMR profile (with HPLC-MS analysis when necessary) can be used as a reference framework for identifying the botanical origin of honey.

  15. Application of statistical process control and process capability analysis procedures in orbiter processing activities at the Kennedy Space Center

    Science.gov (United States)

    Safford, Robert R.; Jackson, Andrew E.; Swart, William W.; Barth, Timothy S.

    1994-01-01

    Successful ground processing at KSC requires that flight hardware and ground support equipment conform to specifications at tens of thousands of checkpoints. Knowledge of conformance is an essential requirement for launch. That knowledge of conformance at every requisite point does not, however, enable identification of past problems with equipment, or potential problem areas. This paper describes how the introduction of Statistical Process Control and Process Capability Analysis identification procedures into existing shuttle processing procedures can enable identification of potential problem areas and candidates for improvements to increase processing performance measures. Results of a case study describing application of the analysis procedures to Thermal Protection System processing are used to illustrate the benefits of the approaches described in the paper.

  16. Country analysis briefs: 1994. Profiles of major world energy producers, consumers, and transport centers

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-05-01

    Country Analysis Briefs: 1994 is a compilation of country profiles prepared by the Energy Markets and Contingency Information Division (EMCID) of the Office of Energy Markets and End Use. EMCID maintains Country Analysis Briefs (CABs) for specific countries or geographical areas that are important to world energy markets. As a general rule, CABs are prepared for all members of the Organization of Petroleum Exporting Countries (OPEC), major non-OPEC oil producers (i.e., the North Sea, Russia), major energy transit areas (i.e., Ukraine), and other areas of current interest to energy analysts and policy makers. As of January 1995, EMCID maintained over 40 CABs, updated on an annual schedule and subject to revision as events warrant. This report includes 25 CABs updated during 1994. All CABs contain a profile section, a map showing the country`s location, and a narrative section. The profile section includes outlines of the country`s economy, energy sector, and environment. The narrative provides further information and discussion of these topics. Some CABs also include a detailed map displaying locations of major oil and gas fields, pipelines, ports, etc. These maps were created as a result of special individual requests and so are not typically a standard feature of the CABs. They are presented here wherever available as a supplement to the information contained in the CABs.

  17. Genome-wide profiling and analysis of Arabidopsis siRNAs.

    Directory of Open Access Journals (Sweden)

    Kristin D Kasschau

    2007-03-01

    Full Text Available Eukaryotes contain a diversified set of small RNA-guided pathways that control genes, repeated sequences, and viruses at the transcriptional and posttranscriptional levels. Genome-wide profiles and analyses of small RNAs, particularly the large class of 24-nucleotide (nt short interfering RNAs (siRNAs, were done for wild-type Arabidopsis thaliana and silencing pathway mutants with defects in three RNA-dependent RNA polymerase (RDR and four Dicer-like (DCL genes. The profiling involved direct analysis using a multiplexed, parallel-sequencing strategy. Small RNA-generating loci, especially those producing predominantly 24-nt siRNAs, were found to be highly correlated with repetitive elements across the genome. These were found to be largely RDR2- and DCL3-dependent, although alternative DCL activities were detected on a widespread level in the absence of DCL3. In contrast, no evidence for RDR2-alternative activities was detected. Analysis of RDR2- and DCL3-dependent small RNA accumulation patterns in and around protein-coding genes revealed that upstream gene regulatory sequences systematically lack siRNA-generating activities. Further, expression profiling suggested that relatively few genes, proximal to abundant 24-nt siRNAs, are regulated directly by RDR2- and DCL3-dependent silencing. We conclude that the widespread accumulation patterns for RDR2- and DCL3-dependent siRNAs throughout the Arabidopsis genome largely reflect mechanisms to silence highly repeated sequences.

  18. Health and Food Safety: Isolation and susceptible profile analysis of pathogenic bacteria of food

    Directory of Open Access Journals (Sweden)

    Lisiane Martins Volcão

    2016-10-01

    Full Text Available Background and Objectives: Currently there is a growing concern about the sanitary conditions of food products made available to population, as there is a wide range of pathogens associated with food contamination. The purpose of this study was to isolation and susceptibility profile analysis of Escherichia coli, Salmonella spp. and Staphylococcus positive coagulase from ground beef samples collected in commerce of Pelotas city, Rio Grande do Sul, Brazil. Methods: For bacterial isolation were utilized 20 samples of ground beef originating in local commerce, being performed the research for E. coli, Salmonella spp. and S. coagulase positive. Any bacterial strains isolated were submitted to analysis of the susceptibility profile, where ten different antibiotics have been tested. Results: The susceptibility profile ranging among isolates in Salmonella spp., we observed the higher rate multidrug resistance, 58% of these were resistant to multiple antibiotics. In isolates of Escherichia coli occurred 44.4% of multidrug resistance. Strains of Staphylococcus positive coagulase showed the lowest rate, 25% of these have been multiresistant, and all isolates were sensitive to oxacillin. Conclusions: The high contamination of samples of ground beef highlights the need for a good hygiene practices and transport animal products. Resistance and multiresistance levels to the antibiotics tested, may demonstrate a possible inappropriate use of antimicrobials in veterinary medicine.

  19. Random analysis of bearing capacity of square footing using the LAS procedure

    Directory of Open Access Journals (Sweden)

    Kawa Marek

    2016-09-01

    Full Text Available In the present paper, a three-dimensional problem of bearing capacity of square footing on random soil medium is analyzed. The random fields of strength parameters c and φ are generated using LAS procedure (Local Average Subdivision, Fenton and Vanmarcke 1990. The procedure used is re-implemented by the authors in Mathematica environment in order to combine it with commercial program. Since the procedure is still tested the random filed has been assumed as one-dimensional: the strength properties of soil are random in vertical direction only.

  20. An alternative method for noise analysis using pixel variance as part of quality control procedures on digital mammography systems.

    NARCIS (Netherlands)

    Bouwman, R.; Young, K.; Lazzari, B.; Ravaglia, V.; Broeders, M.J.M.; Engen, R. van

    2009-01-01

    According to the European Guidelines for quality assured breast cancer screening and diagnosis, noise analysis is one of the measurements that needs to be performed as part of quality control procedures on digital mammography systems. However, the method recommended in the European Guidelines does

  1. CSS Need Analysis, Theory and Computation Procedures for the 1977-78 PCS and FAF, Including Sample Cases and Tables.

    Science.gov (United States)

    College Entrance Examination Board, New York, NY. Coll. Scholarship Service.

    The College Scholarship Service (CSS) need analysis system is described in this comprehensive guide. It includes information about the basic rationale, the economic principles, and the computational procedures used in the CSS system. The chapters deal with the following: principles and practices of student financial aid administration; the history…

  2. Investigating the Structure of the WJ-III Cognitive in Early School Age through Two Exploratory Bifactor Analysis Procedures

    Science.gov (United States)

    Dombrowski, Stefan C.

    2014-01-01

    Two exploratory bifactor methods (e.g., Schmid-Leiman [SL] and exploratory bifactor analysis [EBFA]) were used to investigate the structure of the Woodcock-Johnson III (WJ-III) Cognitive in early school age (age 6-8). The SL procedure is recognized by factor analysts as a preferred method for EBFA. Jennrich and Bentler recently developed an…

  3. 78 FR 14457 - Guidelines Establishing Test Procedures for the Analysis of Pollutants Under the Clean Water Act...

    Science.gov (United States)

    2013-03-06

    ... Clean Water Act; Analysis and Sampling Procedures; Notice AGENCY: Environmental Protection Agency (EPA... Technology, Office of Water (4303-T), Environmental Protection Agency, 1200 Pennsylvania Avenue NW..., soaps, waxes, and hydraulic oil and vegetable based compounds such as cooking oil and other fats. Oil...

  4. APPLICATION OF RADIORECEPTOR ASSAYS FOR SYSTEMATIC TOXICOLOGICAL ANALYSIS .1. PROCEDURES FOR RADIORECEPTOR ASSAYS FOR ANTIHISTAMINICS, ANTICHOLINERGICS AND BENZODIAZEPINES

    NARCIS (Netherlands)

    ENSING, K; BOSMAN, IJ; EGBERTS, ACG; FRANKE, JP; DEZEEUW, RA

    Radioreceptor assays can be a useful tool for systematic toxicological analysis in that they can be applied for the detection of an entire pharmacological class of drugs. In the present paper procedures for radioreceptor assays for benzodiazepines, anticholinergics and antihistaminics have been

  5. A Person-Centered Perspective on Multidimensional Perfectionism in Canadian and Chinese University Students: A Multigroup Latent Profile Analysis

    Science.gov (United States)

    Smith, Martin M.; Saklofske, Donald H.; Yan, Gonggu; Sherry, Simon B.

    2016-01-01

    This study investigated the generalizability of the tripartite model of perfectionism across Canadian and Chinese university students. Using latent profile analysis and indicators of perfectionistic strivings, perfectionistic concerns, and neuroticism in both groups, the authors derived a 3-profile solution: adaptive perfectionists, maladaptive…

  6. Analysis of stranded information using an automated procedure for strand specific RNA sequencing.

    Science.gov (United States)

    Sigurgeirsson, Benjamín; Emanuelsson, Olof; Lundeberg, Joakim

    2014-07-28

    Strand specific RNA sequencing is rapidly replacing conventional cDNA sequencing as an approach for assessing information about the transcriptome. Alongside improved laboratory protocols the development of bioinformatical tools is steadily progressing. In the current procedure the Illumina TruSeq library preparation kit is used, along with additional reagents, to make stranded libraries in an automated fashion which are then sequenced on Illumina HiSeq 2000. By the use of freely available bioinformatical tools we show, through quality metrics, that the protocol is robust and reproducible. We further highlight the practicality of strand specific libraries by comparing expression of strand specific libraries to non-stranded libraries, by looking at known antisense transcription of pseudogenes and by identifying novel transcription. Furthermore, two ribosomal depletion kits, RiboMinus and RiboZero, are compared and two sequence aligners, Tophat2 and STAR, are also compared. The, non-stranded, Illumina TruSeq kit can be adapted to generate strand specific libraries and can be used to access detailed information on the transcriptome. The RiboZero kit is very effective in removing ribosomal RNA from total RNA and the STAR aligner produces high mapping yield in a short time. Strand specific data gives more detailed and correct results than does non-stranded data as we show when estimating expression values and in assembling transcripts. Even well annotated genomes need improvements and corrections which can be achieved using strand specific data. Researchers in the field should strive to use strand specific data; it allows for more confidence in the data analysis and is less likely to lead to false conclusions. If faced with analysing non-stranded data, researchers should be well aware of the caveats of that approach.

  7. Scientific recognition in numbers: analysis of the profile of a vanguard from Lattes curricula vitae

    Directory of Open Access Journals (Sweden)

    Miguel Ângelo Montagner

    2010-01-01

    Full Text Available The objective was to analyze the possibilities and limitations of quantitative study on an institution and its professionals, taking into account the Lattes curriculum vitae platform. Through exploring this material, we sought to delineate the profile of professors of the UNICAMP School of Medical Sciences and to characterize their scientific production, main activities and academic profile. The methodology used was quantitative. Based on descriptive statistics, we outlined and analyzed the main characteristics these professors presented in their curricula vitae, which are stored in the database of the Lattes Platform. Next, using qualitative analysis based on the theories of Pierre Bourdieu, we emphasized the difficulties in achieving good descriptions of researchers' social and scientific paths based on the Lattes Platform. Thus, the Lattes system consists of a repository of finished scientific actions, comprising a linear, non-historical succession within official science.

  8. Experimental analysis of bruises in human volunteers using radiometric depth profiling and diffuse reflectance spectroscopy

    Science.gov (United States)

    Vidovič, Luka; Milanič, Matija; Majaron, Boris

    2015-07-01

    We combine pulsed photothermal radiometry (PPTR) depth profiling with diffuse reflectance spectroscopy (DRS) measurements for a comprehensive analysis of bruise evolution in vivo. While PPTR enables extraction of detailed depth distribution and concentration profiles of selected absorbers (e.g. melanin, hemoglobin), DRS provides information in a wide range of visible wavelengths and thus offers an additional insight into dynamics of the hemoglobin degradation products. Combining the two approaches enables us to quantitatively characterize bruise evolution dynamics. Our results indicate temporal variations of the bruise evolution parameters in the course of bruise self-healing process. The obtained parameter values and trends represent a basis for a future development of an objective technique for bruise age determination.

  9. Multiorientation/multiscript scene text detection based on projection profile analysis and graph segmentation

    Science.gov (United States)

    Koo, Hyung Il

    2016-11-01

    Textline detection in natural images has been an important problem and researchers have attempted to address this problem by grouping connected components (CCs) into clusters corresponding to textlines. However, developing bottom-up rules that work for multiorientation and/or multiscript textlines is not a simple task. In order to address this problem, we propose a framework that incorporates projection profile analysis (PPA) into the CC-based approach. Specifically, we build a graph of CCs and recursively partition the graph into subgraphs, until textline structures are detected by PPA. Although PPA has been a common technique in document image processing, it was developed for scanned documents, and we also propose a method to compute projection profiles for CCs. Experimental results show that our method is efficient and achieves better or comparable performance on conventional datasets (ICDAR 2011/2013 and MSRA-TD500), and shows promising results on a challenging dataset (ICDAR 2015 incidental text localization dataset).

  10. Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray.

    Science.gov (United States)

    Chung, Eun Jung; Sung, Young Kwan; Farooq, Mohammad; Kim, Younghee; Im, Sanguk; Tak, Won Young; Hwang, Yoon Jin; Kim, Yang Il; Han, Hyung Soo; Kim, Jung-Chul; Kim, Moon Kyu

    2002-12-31

    We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.

  11. Minimally invasive procedure reduces adjacent segment degeneration and disease: New benefit-based global meta-analysis.

    Science.gov (United States)

    Li, Xiao-Chuan; Huang, Chun-Ming; Zhong, Cheng-Fan; Liang, Rong-Wei; Luo, Shao-Jian

    2017-01-01

    Adjacent segment pathology (ASP) is a common complication presenting in patients with axial pain and dysfunction, requiring treatment or follow-up surgery. However, whether minimally invasive surgery (MIS), including MIS transforaminal / posterior lumbar interbody fusion (MIS-TLIF/PLIF) decreases the incidence rate of ASP remains unknown. The aim of this meta-analysis was to compare the incidence rate of ASP in patients undergoing MIS versus open procedures. This systematic review was undertaken by following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement. We searched electronic databases, including PubMed, EMBASE, SinoMed, and the Cochrane Library, without language restrictions, to identify clinical trials comparing MIS to open procedures. The results retrieved were last updated on June 15, 2016. Overall, 9 trials comprising 770 patients were included in the study; the quality of the studies included 4 moderate and 5 low-quality studies. The pooled data analysis demonstrated low heterogeneity between the trials and a significantly lower ASP incidence rate in patients who underwent MIS procedure, compared with those who underwent open procedure (p = 0.0001). Single-level lumbar interbody fusion was performed in 6 trials of 408 patients and we found a lower ASP incidence rate in MIS group, compared with those who underwent open surgery (p = 0.002). Moreover, the pooled data analysis showed a significant reduction in the incidence rate of adjacent segment disease (ASDis) (p = 0.0003) and adjacent segment degeneration (ASDeg) (p = 0.0002) for both procedures, favoring MIS procedure. Subgroup analyses showed no difference in follow-up durations between the procedures (p = 0.93). Therefore, we conclude that MIS-TLIF/PLIF can reduce the incidence rate of ASDis and ASDeg, compared with open surgery. Although the subgroup analysis did not indicate a difference in follow-up duration between the two procedures, larger-scale, well

  12. A sensitivity analysis comparing two procedures for adjusting as-measured spectra to reference conditions

    Science.gov (United States)

    2002-04-01

    The Society of Automotive Engineers (SAE) Aerospace Recommended Practice (ARP) No. 866A (866A), and a : procedure utilizing pure-tone absorption equations developed in support of the International Organization : for Standardizations (ISO) 9613-...

  13. Development of a standard operating procedure for analysis of ammonia concentrations in coal fly ash.

    Science.gov (United States)

    2015-04-01

    Research was performed to support the development and recommendation of a standard operating : procedure (SOP) for analyzing the ammonia content in fly ash intended for use in concrete. A review : of existing ash producers found that several differen...

  14. From Laser Scanning to Finite Element Analysis of Complex Buildings by Using a Semi-Automatic Procedure

    Science.gov (United States)

    Castellazzi, Giovanni; D’Altri, Antonio Maria; Bitelli, Gabriele; Selvaggi, Ilenia; Lambertini, Alessandro

    2015-01-01

    In this paper, a new semi-automatic procedure to transform three-dimensional point clouds of complex objects to three-dimensional finite element models is presented and validated. The procedure conceives of the point cloud as a stacking of point sections. The complexity of the clouds is arbitrary, since the procedure is designed for terrestrial laser scanner surveys applied to buildings with irregular geometry, such as historical buildings. The procedure aims at solving the problems connected to the generation of finite element models of these complex structures by constructing a fine discretized geometry with a reduced amount of time and ready to be used with structural analysis. If the starting clouds represent the inner and outer surfaces of the structure, the resulting finite element model will accurately capture the whole three-dimensional structure, producing a complex solid made by voxel elements. A comparison analysis with a CAD-based model is carried out on a historical building damaged by a seismic event. The results indicate that the proposed procedure is effective and obtains comparable models in a shorter time, with an increased level of automation. PMID:26225978

  15. Arthroscopic Latarjet procedure: is optimal positioning of the bone block and screws possible? A prospective computed tomography scan analysis.

    Science.gov (United States)

    Kany, Jean; Flamand, Olivier; Grimberg, Jean; Guinand, Régis; Croutzet, Pierre; Amaravathi, Rajkumar; Sekaran, Padmanaban

    2016-01-01

    We hypothesized that the arthroscopic Latarjet procedure could be performed with accurate bone block positioning and screw fixation with a similar rate of complications to the open Latarjet procedure. In this prospective study, 105 shoulders (104 patients) underwent the arthroscopic Latarjet procedure performed by the same senior surgeon. The day after surgery, an independent surgeon examiner performed a multiplanar bidimensional computed tomography scan analysis. We also evaluated our learning curve by comparing 2 chronologic periods (30 procedures performed in each period), separated by an interval during which 45 procedures were performed. Of the 105 shoulders included in the study, 95 (90.5%) (94 patients) were evaluated. The coracoid graft was accurately positioned relative to the equator of the glenoid surface in 87 of 95 shoulders (91.5%). Accurate bone-block positioning on the axial view with "circle" evaluation was obtained for 77 of 95 shoulders (81%). This procedure was performed in a lateralized position in 7 of 95 shoulders (7.3%) and in a medialized position in 11 shoulders (11.6%). The mean screw angulation with the glenoid surface was 21°. One patient had transient axillary nerve palsy. Of the initial 104 patients, 3 (2.8%) underwent revision. The analysis of our results indicated that the screw-glenoid surface angle significantly predicted the accuracy of the bone-block positioning (P = .001). Our learning curve estimates showed that, compared with our initial period, the average surgical time decreased, and the risk of lateralization showed a statistically significant decrease during the last period (P = .006). This study showed that accurate positioning of the bone block onto the anterior aspect of the glenoid is possible, safe, and reproducible with the arthroscopic Latarjet procedure without additional complications compared with open surgery. Copyright © 2016 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc

  16. Complications of cerebral angiography: a prospective analysis of 2,924 consecutive procedures

    Energy Technology Data Exchange (ETDEWEB)

    Dawkins, A.A.; Evans, A.L.; Wattam, J.; Romanowski, C.A.J.; Connolly, D.J.A.; Hodgson, T.J.; Coley, S.C. [Royal Hallamshire Hospital, Department of Radiology, Sheffield (United Kingdom)

    2007-09-15

    Cerebral angiography is an invasive procedure associated with a small, but definite risk of neurological morbidity. In this study we sought to establish the nature and rate of complications at our institution among a large prospective cohort of consecutive patients. Also, the data were analysed in an attempt to identify risk factors for complications associated with catheter angiography. Data were prospectively collected for a consecutive cohort of patients undergoing diagnostic cerebral angiography between January 2001 and May 2006. A total of 2,924 diagnostic cerebral angiography procedures were performed during this period. The following data were recorded for each procedure: date of procedure, patient age and sex, clinical indication, referring specialty, referral status (routine/emergency), operator, angiographic findings, and the nature of any clinical complication or asymptomatic adverse event (arterial dissection). Clinical complications occurred in 23 (0.79%) of the angiographic procedures: 12 (0.41%) significant puncture-site haematomas, 10 (0.34%) transient neurological events, and 1 nonfatal reaction to contrast agent. There were no permanent neurological complications. Asymptomatic technical complications occurred in 13 (0.44%) of the angiographic procedures: 3 groin dissections and 10 dissections of the cervical vessels. No patient with a neck dissection suffered an immediate or delayed stroke. Emergency procedures (P = 0.0004) and angiography procedures performed for intracerebral haemorrhage (P = 0.02) and subarachnoid haemorrhage (P = 0.04) were associated with an increased risk of complications. Neurological complications following cerebral angiography are rare (0.34%), but must be minimized by careful case selection and the prudent use of alternative noninvasive angiographic techniques, particularly in the acute setting. The low complication rate in this series was largely due to the favourable case mix. (orig.)

  17. Analysis of four dental alloys following torch/centrifugal and induction/ vacuum-pressure casting procedures.

    Science.gov (United States)

    Thompson, Geoffrey A; Luo, Qing; Hefti, Arthur

    2013-12-01

    Previous studies have shown casting methodology to influence the as-cast properties of dental casting alloys. It is important to consider clinically important mechanical properties so that the influence of casting can be clarified. The purpose of this study was to evaluate how torch/centrifugal and inductively cast and vacuum-pressure casting machines may affect the castability, microhardness, chemical composition, and microstructure of 2 high noble, 1 noble, and 1 base metal dental casting alloys. Two commonly used methods for casting were selected for comparison: torch/centrifugal casting and inductively heated/ vacuum-pressure casting. One hundred and twenty castability patterns were fabricated and divided into 8 groups. Four groups were torch/centrifugally cast in Olympia (O), Jelenko O (JO), Genesis II (G), and Liberty (L) alloys. Similarly, 4 groups were cast in O, JO, G, and L by an inductively induction/vacuum-pressure casting machine. Each specimen was evaluated for casting completeness to determine a castability value, while porosity was determined by standard x-ray techniques. Each group was metallographically prepared for further evaluation that included chemical composition, Vickers microhardness, and grain analysis of microstructure. Two-way ANOVA was used to determine significant differences among the main effects. Statistically significant effects were examined further with the Tukey HSD procedure for multiple comparisons. Data obtained from the castability experiments were non-normal and the variances were unequal. They were analyzed statistically with the Kruskal-Wallis rank sum test. Significant results were further investigated statistically with the Steel-Dwass method for multiple comparisons (α=.05). The alloy type had a significant effect on surface microhardness (Pcasting did not affect the microhardness of the test specimen (P=.465). Similarly, the interaction between the alloy and casting technique was not significant (P=.119). A high

  18. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling

    Science.gov (United States)

    Riordan, Daniel P.; Varma, Sushama; West, Robert B.; Brown, Patrick O.

    2015-01-01

    Characterization of the molecular attributes and spatial arrangements of cells and features within complex human tissues provides a critical basis for understanding processes involved in development and disease. Moreover, the ability to automate steps in the analysis and interpretation of histological images that currently require manual inspection by pathologists could revolutionize medical diagnostics. Toward this end, we developed a new imaging approach called multidimensional microscopic molecular profiling (MMMP) that can measure several independent molecular properties in situ at subcellular resolution for the same tissue specimen. MMMP involves repeated cycles of antibody or histochemical staining, imaging, and signal removal, which ultimately can generate information analogous to a multidimensional flow cytometry analysis on intact tissue sections. We performed a MMMP analysis on a tissue microarray containing a diverse set of 102 human tissues using a panel of 15 informative antibody and 5 histochemical stains plus DAPI. Large-scale unsupervised analysis of MMMP data, and visualization of the resulting classifications, identified molecular profiles that were associated with functional tissue features. We then directly annotated H&E images from this MMMP series such that canonical histological features of interest (e.g. blood vessels, epithelium, red blood cells) were individually labeled. By integrating image annotation data, we identified molecular signatures that were associated with specific histological annotations and we developed statistical models for automatically classifying these features. The classification accuracy for automated histology labeling was objectively evaluated using a cross-validation strategy, and significant accuracy (with a median per-pixel rate of 77% per feature from 15 annotated samples) for de novo feature prediction was obtained. These results suggest that high-dimensional profiling may advance the development of computer

  19. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.

    Directory of Open Access Journals (Sweden)

    Daniel P Riordan

    Full Text Available Characterization of the molecular attributes and spatial arrangements of cells and features within complex human tissues provides a critical basis for understanding processes involved in development and disease. Moreover, the ability to automate steps in the analysis and interpretation of histological images that currently require manual inspection by pathologists could revolutionize medical diagnostics. Toward this end, we developed a new imaging approach called multidimensional microscopic molecular profiling (MMMP that can measure several independent molecular properties in situ at subcellular resolution for the same tissue specimen. MMMP involves repeated cycles of antibody or histochemical staining, imaging, and signal removal, which ultimately can generate information analogous to a multidimensional flow cytometry analysis on intact tissue sections. We performed a MMMP analysis on a tissue microarray containing a diverse set of 102 human tissues using a panel of 15 informative antibody and 5 histochemical stains plus DAPI. Large-scale unsupervised analysis of MMMP data, and visualization of the resulting classifications, identified molecular profiles that were associated with functional tissue features. We then directly annotated H&E images from this MMMP series such that canonical histological features of interest (e.g. blood vessels, epithelium, red blood cells were individually labeled. By integrating image annotation data, we identified molecular signatures that were associated with specific histological annotations and we developed statistical models for automatically classifying these features. The classification accuracy for automated histology labeling was objectively evaluated using a cross-validation strategy, and significant accuracy (with a median per-pixel rate of 77% per feature from 15 annotated samples for de novo feature prediction was obtained. These results suggest that high-dimensional profiling may advance the

  20. Short tandem repeat analysis of host's hepatocellular carcinoma by laser microdissection confirms the validity of safety procedures in liver transplantation: a forensic case.

    Science.gov (United States)

    Ventura, Francesco; Bonsignore, Alessandro; Tettamanti, Camilla; Verdiani, Simonetta; Gianelli Castiglione, Andrea; De Stefano, Francesco

    2013-08-09

    To limit the chimerism typical of transplanted organs, which constantly reveals mixed profiles, laser microdissection (LCM) has been hypothesized as a valid tool in comparison with manual dissection. A 42-year-old man with end-stage HBV/HDV liver cirrhosis and single hepatocellular carcinoma (HCC) underwent liver transplantation. Four months later hepatic nodules were diagnosed. The histological investigation showed an HCC. Despite therapy, the man died as a result of metastatic carcinoma 9 months later. On behalf of the public prosecutor, we performed short tandem repeat analysis on the hepatic nodules to determine whether the carcinoma had originated from the transplanted liver. The manually dissected samples revealed a high degree of chimerism that did not allow a clear diagnosis. Instead, the detected chimerism was very low in the microdissected samples, where the tumor origin was clearly diagnosable as a recurrence of the recipient's primitive HCC. Accordingly, the application of LCM improved the quality of the results leading to an exclusion of medical liability profiles, confirming the high quality safety procedure of the Italian system in solid organ transplantation, and showing at the same time how useful this technique may be in selected forensic cases.

  1. Source Attribution of Cyanides using Anionic Impurity Profiling, Stable Isotope Ratios, Trace Elemental Analysis and Chemometrics

    Energy Technology Data Exchange (ETDEWEB)

    Mirjankar, Nikhil S.; Fraga, Carlos G.; Carman, April J.; Moran, James J.

    2016-01-08

    Chemical attribution signatures (CAS) for chemical threat agents (CTAs) are being investigated to provide an evidentiary link between CTAs and specific sources to support criminal investigations and prosecutions. In a previous study, anionic impurity profiles developed using high performance ion chromatography (HPIC) were demonstrated as CAS for matching samples from eight potassium cyanide (KCN) stocks to their reported countries of origin. Herein, a larger number of solid KCN stocks (n = 13) and, for the first time, solid sodium cyanide (NaCN) stocks (n = 15) were examined to determine what additional sourcing information can be obtained through anion, carbon stable isotope, and elemental analyses of cyanide stocks by HPIC, isotope ratio mass spectrometry (IRMS), and inductively coupled plasma optical emission spectroscopy (ICP-OES), respectively. The HPIC anion data was evaluated using the variable selection methods of Fisher-ratio (F-ratio), interval partial least squares (iPLS), and genetic algorithm-based partial least squares (GAPLS) and the classification methods of partial least squares discriminate analysis (PLSDA), K nearest neighbors (KNN), and support vector machines discriminate analysis (SVMDA). In summary, hierarchical cluster analysis (HCA) of anion impurity profiles from multiple cyanide stocks from six reported country of origins resulted in cyanide samples clustering into three groups: Czech Republic, Germany, and United States, independent of the associated alkali metal (K or Na). The three country groups were independently corroborated by HCA of cyanide elemental profiles and corresponded to countries with known solid cyanide factories. Both the anion and elemental CAS are believed to originate from the aqueous alkali hydroxides used in cyanide manufacture. Carbon stable isotope measurements resulted in two clusters: Germany and United States (the single Czech stock grouped with United States stocks). The carbon isotope CAS is believed to

  2. Latent profile analysis of regression-based norms demonstrates relationship of compounding MS symptom burden and negative work events.

    Science.gov (United States)

    Frndak, Seth E; Smerbeck, Audrey M; Irwin, Lauren N; Drake, Allison S; Kordovski, Victoria M; Kunker, Katrina A; Khan, Anjum L; Benedict, Ralph H B

    2016-10-01

    We endeavored to clarify how distinct co-occurring symptoms relate to the presence of negative work events in employed multiple sclerosis (MS) patients. Latent profile analysis (LPA) was utilized to elucidate common disability patterns by isolating patient subpopulations. Samples of 272 employed MS patients and 209 healthy controls (HC) were administered neuroperformance tests of ambulation, hand dexterity, processing speed, and memory. Regression-based norms were created from the HC sample. LPA identified latent profiles using the regression-based z-scores. Finally, multinomial logistic regression tested for negative work event differences among the latent profiles. Four profiles were identified via LPA: a common profile (55%) characterized by slightly below average performance in all domains, a broadly low-performing profile (18%), a poor motor abilities profile with average cognition (17%), and a generally high-functioning profile (9%). Multinomial regression analysis revealed that the uniformly low-performing profile demonstrated a higher likelihood of reported negative work events. Employed MS patients with co-occurring motor, memory and processing speed impairments were most likely to report a negative work event, classifying them as uniquely at risk for job loss.

  3. The analysis of pollutant profiles by means of compositional analysis. An application to PCDD/F data.

    Science.gov (United States)

    Real, Carlos; Fernández, J Angel; Aboal, Jesús R; Carballeira, Alejo

    2009-11-01

    Compositional analysis consists of a group of techniques used to manipulate closed (=compositional) data, i.e. multivariate data summing to a fixed quantity (proportions, percentages). It is based on the analysis of the relations among variables and the use of logarithmic transformations. It has been claimed that this group of techniques should be used to analyse profiles of pollutant sources because profiles themselves are proportions. We show in this paper that, for the exploratory analysis of these data, a good strategy is to combine the analysis done with and without transformation because they give different and complementary insights on the structure of the data. We discuss in particular the study of processes such as the mixing of pollutants produced by different sources and the exponential decay of concentrations with distance to the source found in many studies. The clr transformation is also appropriate for the study of the variables having small proportions, which remain concealed by the abundant variables when analysed without transformation. We present simulations to illustrate these ideas and we also apply these techniques to two data sets of PCDD/F content in moss tissues.

  4. Cost-effectiveness analysis comparing the essure tubal sterilization procedure and laparoscopic tubal sterilization.

    Science.gov (United States)

    Thiel, John A; Carson, George D

    2008-07-01

    To analyze the financial implications of establishing a hysteroscopic sterilization program using the Essure micro-insert tubal sterilization system in an ambulatory clinic. A retrospective cohort study (Canadian Task Force classification Type II-2), in an ambulatory women's health clinic in a tertiary hospital, of 108 women undergoing Essure coil insertion between 2005 and 2006, and 104 women undergoing laparoscopic tubal sterilization for permanent sterilization between 2001 and 2004. The Essure procedures used a 4 mm single channel operative hysteroscope and conscious sedation (fentanyl and midazolam); the laparoscopic tubal sterilizations were completed under general anaesthesia with a 7 mm laparoscope and either bipolar cautery or Filshie clips. Costs associated with the procedure, follow-up, and management of any complications (including nursing, hospital charges, equipment, and disposables) were tabulated. The Essure coils were successfully placed on the first attempt in 103 of 108 women (95%). Three patients required a second attempt to complete placement and two patients required laparoscopic tubal sterilization after an unsuccessful Essure. All 104 laparoscopic tubals were completed on the first attempt with no complications reported. The total cost for the 108 Essure procedures, including follow-up evaluation, was $138,996 or $1287 per case. The total cost associated with the 104 laparoscopic tubal sterilization procedures was $148,227 or $1398 per case. The incremental cost-effectiveness ratio was $111. The Essure procedure in an ambulatory setting resulted in a statistically significant cost saving of $111 per sterilization procedure. Carrying out the Essure procedure in an ambulatory setting frees space in the operating room for other types of cases, improving access to care for more patients.

  5. The Latarjet Procedure at the National Football League Scouting Combine: An Imaging and Performance Analysis.

    Science.gov (United States)

    LeBus, George F; Chahla, Jorge; Sanchez, George; Akamefula, Ramesses; Moatshe, Gilbert; Phocas, Alexandra; Price, Mark D; Whalen, James M; LaPrade, Robert F; Provencher, Matthew T

    2017-09-01

    The Latarjet procedure is commonly performed in the setting of glenoid bone loss for treatment of recurrent anterior shoulder instability; however, little is known regarding the outcomes of this procedure in elite American football players. (1) Determine the prevalence, clinical features, and imaging findings of elite college football athletes who present to the National Football League (NFL) Combine with a previous Latarjet procedure and (2) describe these athletes' performance in the NFL in terms of draft status and initial playing time. Case series; Level of evidence, 4. After review of all football players who participated in the NFL Combine from 2009 to 2016, any player with a previous Latarjet procedure was included in this study. Medical records, position on the field, and draft position were recorded for each player. In addition, imaging studies were reviewed to determine fixation type, hardware complications, and status of the bone block. For those players who were ultimately drafted, performance was assessed based on games played and started, total snaps, and percentage of eligible snaps in which the player participated during his rookie season. Overall, 13 of 2617 (Latarjet procedure. Radiographically, 8 of 13 (61%) showed 2-screw fixation, while 5 of 13 (39%) had 1 screw. Of the 13 players, 6 (46%) players demonstrated hardware complications. All players had evidence of degenerative changes on plain radiographs, with 10 (77%) graded as mild, 1 (8%) as moderate, and 2 (15%) as severe according to the Samilson Prieto classification. Six of the 13 (46%) players went undrafted, while the remaining 7 (54%) were drafted; however, no player participated in more than half of the plays for which he was eligible during his rookie season. Only a small percentage of players at the NFL Combine (Latarjet procedure. High rates of postoperative complications and radiographically confirmed degenerative change were observed. Athletes who had undergone a Latarjet

  6. Latent profile analysis of students’ motivation and outcomes in mathematics: an organismic integration theory perspective

    Directory of Open Access Journals (Sweden)

    Chee Keng John Wang

    2017-05-01

    Full Text Available The purpose of the current study was to identify the motivation profiles at the intraindividual level using a latent profile analyses (LPA approach. A total of 1151 secondary school students aged 13 to 17 years old from Singapore took part in the study. Using LPA, four distinct motivational profiles were identified based on four motivation regulations. Profile 1 has very low introjected and low autonomous motivation (6% of sample. Profile 2 had high external and identified regulations and very low intrinsic regulation (10%. Profile 3 consisted of students with high identified and intrinsic regulations (51%. Profile 4 had moderately low identified and intrinsic regulations (33%. The results showed that the four profiles differed significantly in terms of effort, competence, value, and time spent on math beyond homework. The best profile (Profile 3 reported highest scores in effort, value, competence and time spent on Math beyond homework. The worst profile (Profile 1 reported lowest scores in all the four outcome variables.

  7. Qualitative analysis of the Clock Drawing Test by educational level and cognitive profile

    Directory of Open Access Journals (Sweden)

    Aline Teixeira Fabricio

    2014-04-01

    Full Text Available The use of a qualitative scale for the Clock Drawing Test (CDT may add information about the pattern of errors committed. Objective: To translate and adapt the Modified Qualitative Error Analysis of Rouleau into Brazilian Portuguese and to examine the pattern of errors according to educational level and cognitive profile. Method: 180 adults (47-82 years completed the CDT. Participants were stratified into age and educational levels and separated between those with and without changes in cognitive screening tests (Mini-Mental State Examination, Verbal Fluency. Results: No significant differences were found in CDT scores among age groups. Among participants without cognitive impairment, those with lower education often presented graphic difficulties, conceptual deficits and spatial deficits. Participants with cognitive deficits, demonstrated more frequently conceptual and spatial errors. Conclusion: The qualitative analysis of the CDT may contribute to the identification of cognitive changes. Education level has to be taken into consideration during the analysis.

  8. Mechanism of Xinfeng Capsule on Adjuvant-Induced Arthritis via Analysis of Urinary Metabolomic Profiles

    Directory of Open Access Journals (Sweden)

    Hui Jiang

    2016-01-01

    Full Text Available We aimed to explore the potential effects of Xinfeng capsule (XFC on urine metabolic profiling in adjuvant-induced arthritis (AA rats by using gas chromatography time-of-flight mass spectrometry (GC-TOF/MS. GC-TOF/MS technology was combined with multivariate statistical approaches, such as principal component analysis (PCA, partial least squares discriminant analysis (PLS-DA, and orthogonal projections to latent structures discriminant analysis (OPLS-DA. These methods were used to distinguish the healthy group, untreated group, and XFC treated group and elucidate potential biomarkers. Nine potential biomarkers such as hippuric acid, adenine, and L-dopa were identified as potential biomarkers, indicating that purine metabolism, fat metabolism, amino acid metabolism, and energy metabolism were disturbed in AA rats. This study demonstrated that XFC is efficacious for RA and explained its potential metabolomics mechanism.

  9. Pragmatic evaluation of the Toyota Production System (TPS analysis procedure for problem solving with entry-level nurses

    Directory of Open Access Journals (Sweden)

    Lukasz Maciej Mazur

    2008-12-01

    Full Text Available Medication errors occurring in hospitals are a growing national concern. These medication errors and their related costs (or wastes are seen as major factors leading to increased patient safety risks and increased waste in the hospital setting.  This article presents a study in which sixteen entry-level nurses utilized a Toyota Production System (TPS analysis procedure to solve medication delivery problems at one community hospital. The objective of this research was to study and evaluate the TPS analysis procedure for problem solving with entry-level nurses. Personal journals, focus group discussions, and a survey study were used to collect data about entry-level nurses’ perceptions of using the TPS problem solving approach to study medication delivery. A regression analysis was used to identify characteristics that enhance problem solving efforts. In addition, propositions for effective problem solving by entry-level nurses to aid in the reduction of medication errors in healthcare delivery settings are offered.

  10. Analysis of adolescent profiles by gender: strengths, attitudes toward violence and sexism.

    Science.gov (United States)

    Ferragut, Marta; Blanca, Maria J; Ortiz-Tallo, Margarita

    2014-02-20

    The present study analyzes the profiles of boys and girls, considering gender, in the early stages of adolescence in the variables of character strengths, attitudes toward diversity and violence, and sexism. The aim is to explore the gender differences, whether the variables in each set differ from one another and whether these differences are maintained in profiles for boys and girls. The participants were 527 students (mean age = 12.21 and SD = 0.53) from the city of Málaga (Spain). Profile analysis was used to analyze data. The results, using an alpha of 0.0021 for each contrast, indicate that boys and girls differ in their character strengths, particularly in the case of girls, whose prominent strengths relate to pro-social behavior and peer relationships, where Cohen´s d are higher than .30. Moreover, boys justify attitudes of violence to a greater extent (Cohen´s d from .44 to .81) and show greater agreement with sexist beliefs (d = .63). The research suggests that it would be of interest to encourage advancement in character strengths at this age.

  11. Computational analysis of epidermal growth factor receptor mutations predicts differential drug sensitivity profiles towards kinase inhibitors.

    Science.gov (United States)

    Akula, Sravani; Kamasani, Swapna; Sivan, SreeKanth; Manga, Vijjulatha; Vudem, Dashavantha Reddy; Kancha, Rama Krishna

    2018-01-21

    A significant proportion of lung cancer patients carry mutations in the epidermal growth factor receptor (EGFR) kinase domain. The presence of a deletion mutation in exon 19 or L858R point mutation in the EGFR kinase domain were shown to cause enhanced efficacy of inhibitor treatment in NSCLC patients. Several less frequent ("Uncommon") mutations in the EGFR kinase domain with potential implications in treatment response were also reported. The role of a limited number of uncommon mutations in drug sensitivity was experimentally verified. However, a huge number of these mutations remain uncharacterized for inhibitor sensitivity/resistance. A large scale computational analysis of clinically reported 298 point mutants of EGFR kinase domain has been performed and drug sensitivity profiles for each mutant towards seven kinase inhibitors has been determined by molecular docking. In addition, the relative inhibitor binding affinity (RIBA) towards each drug as compared to that of the ATP was calculated for each mutant. The inhibitor sensitivity profiles predicted in this study for a set of previously characterized mutants correlated well with the published clinical, experimental and computational data. Both the single and compound mutations displayed differential inhibitor sensitivity towards first and next generation kinase inhibitors. The present study provides predicted drug sensitivity profiles for a large panel of uncommon EGFR mutations towards multiple inhibitors which may help clinicians in deciding mutant-specific treatment strategies. Copyright © 2018 International Association for the Study of Lung Cancer. Published by Elsevier Inc. All rights reserved.

  12. Emotional labor actors: a latent profile analysis of emotional labor strategies.

    Science.gov (United States)

    Gabriel, Allison S; Daniels, Michael A; Diefendorff, James M; Greguras, Gary J

    2015-05-01

    Research on emotional labor focuses on how employees utilize 2 main regulation strategies-surface acting (i.e., faking one's felt emotions) and deep acting (i.e., attempting to feel required emotions)-to adhere to emotional expectations of their jobs. To date, researchers largely have considered how each strategy functions to predict outcomes in isolation. However, this variable-centered perspective ignores the possibility that there are subpopulations of employees who may differ in their combined use of surface and deep acting. To address this issue, we conducted 2 studies that examined surface acting and deep acting from a person-centered perspective. Using latent profile analysis, we identified 5 emotional labor profiles-non-actors, low actors, surface actors, deep actors, and regulators-and found that these actor profiles were distinguished by several emotional labor antecedents (positive affectivity, negative affectivity, display rules, customer orientation, and emotion demands-abilities fit) and differentially predicted employee outcomes (emotional exhaustion, job satisfaction, and felt inauthenticity). Our results reveal new insights into the nature of emotion regulation in emotional labor contexts and how different employees may characteristically use distinct combinations of emotion regulation strategies to manage their emotional expressions at work. (c) 2015 APA, all rights reserved.

  13. Temporal Profile of Biofilm Formation, Gene Expression and Virulence Analysis in Candida albicans Strains.

    Science.gov (United States)

    de Barros, Patrícia Pimentel; Rossoni, Rodnei Dennis; De Camargo Ribeiro, Felipe; Junqueira, Juliana Campos; Jorge, Antonio Olavo Cardoso

    2017-04-01

    The characterization of Candida albicans strains with different degrees of virulence became very useful to understand the mechanisms of fungal virulence. Then, the objective of this study was to assess and compare the temporal profiles of biofilms formation, gene expression of ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 and virulence in Galleria mellonella of C. albicans ATCC18804 and a clinical sample isolated from an HIV-positive patient (CA60). Although the CFU/mL counting was higher in biofilms formed in vitro by ATCC strain, the temporal profile of the analysis of the transcripts of the C. albicans strains was elevated to Ca60 compared to strain ATCC, especially in the genes HWP1, ALS3, SAP5, PLB2 and LIP9 (up regulation). Ca60 was more pathogenic for G. mellonella in the survival assay (p = 0.0394) and hemocytes density (p = 0.0349), agreeing with upregulated genes that encode the expression of hyphae and hydrolase genes of Ca60. In conclusion, the C. albicans strains used in this study differ in the amount of biofilm formation, virulence in vivo and transcriptional profiles of genes analyzed that can change factors associated with colonization, proliferation and survival of C. albicans at different niches. SAP5 and HWP1 were the genes more expressed in the formation of biofilm in vitro.

  14. The Re-Analysis of Ozone Profile Data from a 41-Year Series of SBUV Instruments

    Science.gov (United States)

    Kramarova, Natalya; Frith, Stacey; Bhartia, Pawan K.; McPeters, Richard; Labow, Gordon; Taylor, Steven; Fisher, Bradford

    2012-01-01

    In this study we present the validation of ozone profiles from a number of Solar Back Scattered Ultra Violet (SBUV) and SBUV/2 instruments that were recently reprocessed using an updated (Version 8.6) algorithm. The SBUV dataset provides the longest available record of global ozone profiles, spanning a 41-year period from 1970 to 2011 (except a 5-year gap in the 1970s) and includes ozone profile records obtained from the Nimbus-4 BUV and Nimbus-7 SBUV instruments, and a series of SBUV(/2) instruments launched on NOAA operational satellites (NOAA 09, 11, 14, 16, 17, 18, 19). Although modifications in instrument design were made in the evolution from the BUV instrument to the modern SBUV(/2) model, the basic principles of the measurement technique and retrieval algorithm remain the same. The long term SBUV data record allows us to create a consistent, calibrated dataset of ozone profiles that can be used for climate studies and trend analyses. In particular, we focus on estimating the various sources of error in the SBUV profile ozone retrievals using independent observations and analysis of the algorithm itself. For the first time we include in the metadata a quantitative estimate of the smoothing error, defined as the error due to profile variability that the SBUV observing system cannot inherently measure. The magnitude of the smoothing error varies with altitude, latitude, season and solar zenith angle. Between 10 and 1 hPa the smoothing errors for the SBUV monthly zonal mean retrievals are of the order of 1 %, but start to increase above and below this layer. The largest smoothing errors, as large as 15-20%, were detected in in the troposphere. The SBUV averaging kernels, provided with the ozone profiles in version 8.6, help to eliminate the smoothing effect when comparing the SBUV profiles with high vertical resolution measurements, and make it convenient to use the SBUV ozone profiles for data assimilation and model validation purposes. The smoothing error can

  15. Infectomic Analysis of Gene Expression Profiles of Human Brain Microvascular Endothelial Cells Infected with Cryptococcus neoformans

    Directory of Open Access Journals (Sweden)

    Ambrose Jong

    2008-01-01

    Full Text Available In order to dissect the pathogenesis of Cryptococcus neoformans meningoencephalitis, a genomic survey of the changes in gene expression of human brain microvascular endothelial cells infected by C. neoformans was carried out in a time-course study. Principal component analysis (PCA revealed sigificant fluctuations in the expression levels of different groups of genes during the pathogen-host interaction. Self-organizing map (SOM analysis revealed that most genes were up- or downregulated 2 folds or more at least at one time point during the pathogen-host engagement. The microarray data were validated by Western blot analysis of a group of genes, including β-actin, Bcl-x, CD47, Bax, Bad, and Bcl-2. Hierarchical cluster profile showed that 61 out of 66 listed interferon genes were changed at least at one time point. Similarly, the active responses in expression of MHC genes were detected at all stages of the interaction. Taken together, our infectomic approaches suggest that the host cells significantly change the gene profiles and also actively participate in immunoregulations of the central nervous system (CNS during C. neoformans infection.

  16. Processing methods for differential analysis of LC/MS profile data

    Directory of Open Access Journals (Sweden)

    Orešič Matej

    2005-07-01

    Full Text Available Abstract Background Liquid chromatography coupled to mass spectrometry (LC/MS has been widely used in proteomics and metabolomics research. In this context, the technology has been increasingly used for differential profiling, i.e. broad screening of biomolecular components across multiple samples in order to elucidate the observed phenotypes and discover biomarkers. One of the major challenges in this domain remains development of better solutions for processing of LC/MS data. Results We present a software package MZmine that enables differential LC/MS analysis of metabolomics data. This software is a toolbox containing methods for all data processing stages preceding differential analysis: spectral filtering, peak detection, alignment and normalization. Specifically, we developed and implemented a new recursive peak search algorithm and a secondary peak picking method for improving already aligned results, as well as a normalization tool that uses multiple internal standards. Visualization tools enable comparative viewing of data across multiple samples. Peak lists can be exported into other data analysis programs. The toolbox has already been utilized in a wide range of applications. We demonstrate its utility on an example of metabolic profiling of Catharanthus roseus cell cultures. Conclusion The software is freely available under the GNU General Public License and it can be obtained from the project web page at: http://mzmine.sourceforge.net/.

  17. Procedural learning is impaired in dyslexia: Evidence from a meta-analysis of serial reaction time studies☆

    Science.gov (United States)

    Lum, Jarrad A.G.; Ullman, Michael T.; Conti-Ramsden, Gina

    2013-01-01

    A number of studies have investigated procedural learning in dyslexia using serial reaction time (SRT) tasks. Overall, the results have been mixed, with evidence of both impaired and intact learning reported. We undertook a systematic search of studies that examined procedural learning using SRT tasks, and synthesized the data using meta-analysis. A total of 14 studies were identified, representing data from 314 individuals with dyslexia and 317 typically developing control participants. The results indicate that, on average, individuals with dyslexia have worse procedural learning abilities than controls, as indexed by sequence learning on the SRT task. The average weighted standardized mean difference (the effect size) was found to be 0.449 (CI95: .204, .693), and was significant (p dyslexia. PMID:23920029

  18. Proteomic analysis of Parietaria judaica pollen and allergen profiling by an immunoproteomic approach.

    Science.gov (United States)

    Barranca, Marilisa; Fontana, Simona; Taverna, Simona; Duro, Giovanni; Zanella-Cleon, Isabelle; Becchi, Michel; De Leo, Giacomo; Alessandro, Riccardo

    2010-04-01

    Parietaria judaica pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature Parietaria judaica pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from Parietaria judaica allergic patients we obtained a more detailed characterization of Parietaria judaica allergen profile so to improve our comprehension of the pathogenesis of pollen-induced allergic reaction.

  19. Directional pair distribution function for diffraction line profile analysis of atomistic models.

    Science.gov (United States)

    Leonardi, Alberto; Leoni, Matteo; Scardi, Paolo

    2013-02-01

    The concept of the directional pair distribution function is proposed to describe line broadening effects in powder patterns calculated from atomistic models of nano-polycrystalline microstructures. The approach provides at the same time a description of the size effect for domains of any shape and a detailed explanation of the strain effect caused by the local atomic displacement. The latter is discussed in terms of different strain types, also accounting for strain field anisotropy and grain boundary effects. The results can in addition be directly read in terms of traditional line profile analysis, such as that based on the Warren-Averbach method.

  20. WEB-THERMODYN: sequence analysis software for profiling DNA helical stability

    OpenAIRE

    Huang, Yanlin; Kowalski, David

    2003-01-01

    WEB-THERMODYN analyzes DNA sequences and computes the DNA helical stability, i.e. the free energy required to unwind and separate the strands of the double helix. A helical stability profile across a selected DNA region or the entire sequence is generated by sliding-window analysis. WEB-THERMODYN can predict sites of low helical stability present at regulatory regions for transcription and replication and can be used to test the influence of mutations. The program can be accessed at: http://w...

  1. Analysis and high resolution modelling of black carbon vertical profiles measured over three Italian valleys

    Science.gov (United States)

    Gandolfi, Ilaria; Curci, Gabriele; Falasca, Serena; Ferrero, Luca

    2017-04-01

    Analysis and high resolution modelling of black carbon vertical profiles measured over three Italian valleys Ilaria Gandolfi1,2, Gabriele Curci1,2, Serena Falasca1,2, Luca Ferrero3 1 Department of Physical and Chemical Sciences, University of L'Aquila, L'Aquila, Italy 2 Center of Excellence CETEMPS, University of L'Aquila, L'Aquila, Italy 3 POLARIS Research Centre, Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy Last decades were characterized by a growing interest in aerosols: mainly for their effect on human health and on the energy balance of solar and planetary radiation, thus their role in climate change. In this study, we analyze the evolution of vertical profile of black carbon (BC) through tethered balloon observations and chemistry-transport modelling. Black carbon is regarded as the second most important anthropogenic climate forcing agent and its concentration varies significantly depending on the altitude and the sources on the territory. In winter of 2010 University Of Milan Bicocca conducted three intensive measurements campaigns over three Italian basin valleys (Terni, Po Valley, Passiria Valley). The choice of the valleys was made taking into consideration the orography and the river basin structure. The measurement campaign was based on a helium-filled tethered balloon, on which the instrumentation for the analysis has been mounted; the instrumentation consisted on a meteorological station, an OPC, a cascade impactor and a micro-Aethalometer. Subsequently, at University of L'Aquila simulations were produced to help interpretation of these vertical aerosol profiles (mass, composition and distribution) and related optical properties (scattering, absorption) using a chemistry-transport model (WRF-CHIMERE) at high horizontal resolution (1 km). The analysis focused primarily on the calculation of the heating rate and of the Direct Radiative Effect (DRE), and on the analysis of the

  2. The Kraske procedure: a critical analysis of a surgical approach for mid-rectal lesions.

    Science.gov (United States)

    Onaitis, Mark; Ludwig, Kirk; Perez-Tamayo, Anthony; Gottfried, Marcia; Russell, Linda; Shadduck, Phillip; Pappas, Theodore; Seigler, Hilliard F; Tyler, Douglas S

    2006-09-01

    To analyze the Kraske procedure as an approach to mid-rectal disease. Twenty-two patients underwent a Kraske procedure at either Duke University Medical Center, the Durham Veterans Administration Medical Center, or the Durham Regional Hospital between 1992 and 1997. The clinical and pathologic characteristics of these patients were retrospectively analyzed and compared with previous published series. Of the 22 patients, 13 underwent resection of an adenocarcinoma and 9 underwent resection of a villous adenoma. Post-operative complications included four fecal fistulas (two of which required a temporary diverting colostomy), two wound infections, two cases of urinary retention, and one case of transient fecal incontinence. The Kraske procedure minimizes exposure of mid-rectal lesions without the morbidity of a major laparotomy. However, it does carry a moderate complication rate and thus should be utilized selectively in managing patients with mid-rectal tumors not amenable to other treatment options. 2006 Wiley-Liss, Inc.

  3. Health economic analysis of laparoscopic lavage versus Hartmann's procedure for diverticulitis in the randomized DILALA trial

    DEFF Research Database (Denmark)

    Gehrman, J.; Angenete, E; Björholt, I.

    2016-01-01

    Background: Open surgery with resection and colostomy (Hartmann's procedure) has been the standard treatment for perforated diverticulitis with purulent peritonitis. In recent years laparoscopic lavage has emerged as an alternative, with potential benefits for patients with purulent peritonitis......, Hinchey grade III. The aim of this study was to compare laparoscopic lavage and Hartmann's procedure with health economic evaluation within the framework of the DILALA (DIverticulitis – LAparoscopic LAvage versus resection (Hartmann's procedure) for acute diverticulitis with peritonitis) trial. Methods...... were robust as demonstrated in sensitivity analyses. Conclusion: The significant cost reduction in this study, together with results of safety and efficacy from RCTs, support the routine use of laparoscopic lavage as treatment for complicated diverticulitis with purulent peritonitis....

  4. Assessment of Patients Referred to Specialty Dental Hospitals for Dental Implant Procedure: A Retrospective Cohort Analysis.

    Science.gov (United States)

    Thukral, Rishi; Kumar, Anuj; Prasant, M C; Punjabi, Krunal M; Paliwal, Aparna; Patel, Kunal

    2016-06-01

    One of the most common and effective ways of replacing missing teeth is by dental implants. Both quality and quantity of bone along with the area of implant placement govern the prognosis of the implant procedure. Certain risk factors predispose the implant treatment to high failure rate. Hence, we assessed the implant patients who were referred from private practitioners to the specialty hospitals from 2010 to 2014. All the patients being referred from private clinics to the specialty dental hospital for the purpose of prosthetic rehabilitation by dental implants from June 2010 to July 2014 were included in the present study. Skilled oral and maxillofacial surgeons were appointed for performing the implant surgical procedures. Prosthetic rehabilitation was done after 6 to 8 weeks and after 10 to 14 weeks in implant cases without and with bone augmentation procedures respectively. Distribution of dental implants based on the indications, location, dimension of augmentation procedure, and complication of implants was analyzed and assessed for the level of significance. Of the patients, 712 were females, while the remaining were males. Most of the patients were in the age group of 50 to 59 years. As compared with completely edentulous patients, most of the patients required rehabilitation by a single implant. Maximum dental implants were placed in maxillary premolar region and mandibular first molar region. Over 1,000 cases in this study required rehabilitation by augmentation procedure. Partially edentulous patients are most commonly referred to specialized dental hospitals for prosthetic rehabilitation by dental implants, mostly with the purpose of implant placement. Failure rate can be minimized by following strict patient selection protocols along with following a standard surgical criterion. Following standard surgical protocols and strict treatment planning, prognosis of the dental implant procedures can be improved to a greater extent, thereby increasing its

  5. External validation of a forest inventory and analysis volume equation and comparisons with estimates from multiple stem-profile models

    Science.gov (United States)

    Christopher M. Oswalt; Adam M. Saunders

    2009-01-01

    Sound estimation procedures are desideratum for generating credible population estimates to evaluate the status and trends in resource conditions. As such, volume estimation is an integral component of the U.S. Department of Agriculture, Forest Service, Forest Inventory and Analysis (FIA) program's reporting. In effect, reliable volume estimation procedures are...

  6. A chemical profiling strategy for semi-quantitative analysis of flavonoids in Ginkgo extracts.

    Science.gov (United States)

    Yang, Jing; Wang, An-Qi; Li, Xue-Jing; Fan, Xue; Yin, Shan-Shan; Lan, Ke

    2016-05-10

    Flavonoids analysis in herbal products is challenged by their vast chemical diversity. This work aimed to develop a chemical profiling strategy for the semi-quantification of flavonoids using extracts of Ginkgo biloba L. (EGB) as an example. The strategy was based on the principle that flavonoids in EGB have an almost equivalent molecular absorption coefficient at a fixed wavelength. As a result, the molecular-contents of flavonoids were able to be semi-quantitatively determined by the molecular-concentration calibration curves of common standards and recalculated as the mass-contents with the characterized molecular weight (MW). Twenty batches of EGB were subjected to HPLC-UV/DAD/MS fingerprinting analysis to test the feasibility and reliability of this strategy. The flavonoid peaks were distinguished from the other peaks with principle component analysis and Pearson correlation analysis of the normalized UV spectrometric dataset. Each flavonoid peak was subsequently tentatively identified by the MS data to ascertain their MW. It was highlighted that the flavonoids absorption at Band-II (240-280 nm) was more suitable for the semi-quantification purpose because of the less variation compared to that at Band-I (300-380 nm). The semi-quantification was therefore conducted at 254 nm. Beyond the qualitative comparison results acquired by common chemical profiling techniques, the semi-quantitative approach presented the detailed compositional information of flavonoids in EGB and demonstrated how the adulteration of one batch was achieved. The developed strategy was believed to be useful for the advanced analysis of herbal extracts with a high flavonoid content without laborious identification and isolation of individual components. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Identification and expression profiling analysis of TCP family genes involved in growth and development in maize.

    Science.gov (United States)

    Chai, Wenbo; Jiang, Pengfei; Huang, Guoyu; Jiang, Haiyang; Li, Xiaoyu

    2017-10-01

    The TCP family is a group of plant-specific transcription factors. TCP genes encode proteins harboring bHLH structure, which is implicated in DNA binding and protein-protein interactions and known as the TCP domain. TCP genes play important roles in plant development and have been evolutionarily and functionally elaborated in various plants, however, no overall phylogenetic analysis or expression profiling of TCP genes in Zea mays has been reported. In the present study, a systematic analysis of molecular evolution and functional prediction of TCP family genes in maize (Z. mays L.) has been conducted. We performed a genome-wide survey of TCP genes in maize, revealing the gene structure, chromosomal location and phylogenetic relationship of family members. Microsynteny between grass species and tissue-specific expression profiles were also investigated. In total, 29 TCP genes were identified in the maize genome, unevenly distributed on the 10 maize chromosomes. Additionally, ZmTCP genes were categorized into nine classes based on phylogeny and purifying selection may largely be responsible for maintaining the functions of maize TCP genes. What's more, microsynteny analysis suggested that TCP genes have been conserved during evolution. Finally, expression analysis revealed that most TCP genes are expressed in the stem and ear, which suggests that ZmTCP genes influence stem and ear growth. This result is consistent with the previous finding that maize TCP genes represses the growth of axillary organs and enables the formation of female inflorescences. Altogether, this study presents a thorough overview of TCP family in maize and provides a new perspective on the evolution of this gene family. The results also indicate that TCP family genes may be involved in development stage in plant growing conditions. Additionally, our results will be useful for further functional analysis of the TCP gene family in maize.

  8. Systematic review and meta-analysis of enterocolitis after one-stage transanal pull-through procedure for Hirschsprung's disease.

    LENUS (Irish Health Repository)

    Ruttenstock, Elke

    2012-02-01

    PURPOSE: The transanal one-stage pull-through procedure (TERPT) has gained worldwide popularity over open and laparoscopic-assisted one-stage techniques in children with Hirschsprung\\'s disease (HD). It offers the advantages of avoiding laparotomy, laparoscopy, scars, abdominal contamination, and adhesions. However, enterocolitis associated with Hirschsprung\\'s disease (HAEC) still remains to be a potentially life-threatening complication after pull-through operation. The reported incidence of HAEC ranges from 4.6 to 54%. This meta-analysis was designed to evaluate postoperative incidence of HAEC following TERPT procedure. METHODS: A meta-analysis of cases of TERPT reported between 1998 and 2009 was performed. Detailed information was recorded regarding intraoperative details and postoperative complications with particular emphasis on incidence of HAEC. Diagnosis of HAEC in a HD patient was based on the clinical presentation of diarrhoea, abdominal distension, and fever. RESULTS: Of the 54 published articles worldwide, 27 articles, including 899 patients were identified as reporting entirely TERPT procedure. Postoperative HAEC occurred in 92 patients (10.2%). Recurrent episodes of HAEC were reported in 18 patients (2%). Conservative treatment of HAEC was successful in 75 patients (81.5%), whereas in 17 patients (18.5%) surgical treatment was needed. CONCLUSIONS: This systematic review reveals that TERPT is a safe and less-invasive procedure with a low incidence of postoperative HAEC.

  9. A Comparative Analysis of British and Taiwanese Students' Conceptual and Procedural Knowledge of Fraction Addition

    Science.gov (United States)

    Li, Hui-Chuan

    2014-01-01

    This study examines students' procedural and conceptual achievement in fraction addition in England and Taiwan. A total of 1209 participants (561 British students and 648 Taiwanese students) at ages 12 and 13 were recruited from England and Taiwan to take part in the study. A quantitative design by means of a self-designed written test is adopted…

  10. Finite element procedures for coupled linear analysis of heat transfer, fluid and solid mechanics

    Science.gov (United States)

    Sutjahjo, Edhi; Chamis, Christos C.

    1993-01-01

    Coupled finite element formulations for fluid mechanics, heat transfer, and solid mechanics are derived from the conservation laws for energy, mass, and momentum. To model the physics of interactions among the participating disciplines, the linearized equations are coupled by combining domain and boundary coupling procedures. Iterative numerical solution strategy is presented to solve the equations, with the partitioning of temporal discretization implemented.

  11. A qualitative analysis of the determinants in the choice of a French journal reviewing procedures

    Science.gov (United States)

    Morge, Ludovic

    2015-12-01

    Between 1993 and 2010, two French journals (Aster and Didaskalia) coming from different backgrounds but belonging to the same institution used to publish papers on research in science and technology education. The merging of these journals made it necessary for them to compare the different reviewing procedures used by each. This merging occurred at a time when research is becoming increasingly international which partly determines some of the reviewing procedure choices. In order for a francophone international journal to survive, it needs to take this internationalization into account in a reasoned manner. The author of this article, as a chief editor of RDST (Recherches en Didactique des Sciences et des Technologies)—the journal resulting from the merging- taking part in this merger, analyses the social, cultural and pragmatic determinants which impacted the choices made in reviewing procedures. This paper describes how these diversity of factors leads us to drop the idea of a standard reviewing procedure which would be valid for all journals.

  12. Soil-contact decay tests using small blocks : a procedural analysis

    Science.gov (United States)

    Rodney C. De Groot; James W. Evans; Paul G. Forsyth; Camille M. Freitag; Jeffrey J. Morrell

    Much discussion has been held regarding the merits of laboratory decay tests compared with field tests to evaluate wood preservatives. In this study, procedural aspects of soil jar decay tests with 1 cm 3 blocks were critically examined. Differences among individual bottles were a major source of variation in this method. The reproducibility and sensitivity of the soil...

  13. Rule of law and participation: a normative analysis of internationalized rulemaking as composite procedures

    NARCIS (Netherlands)

    Mendes, J.

    2014-01-01

    Procedural standards of participation have the capacity to structure and constrain the exercise of authority. Focusing on the way decisions are formed, this article argues that the depletion of such standards in processes of reception of transnational and international decisions within the EU

  14. Standard operating procedure: implementation, critical analysis, and validation in the Audiology Department at CESTEH/Fiocruz.

    Science.gov (United States)

    Freitas, Anelisse Vasco Mascarenhas de; Quixabeiro, Elinaldo Leite; Luz, Geórgia Rosangela Soares; Franco, Viviane Moreira; Santos, Viviane Fontes Dos

    2016-01-01

    Evaluate three standard operational procedures (SOPs), regarding the application of the brainstem auditory evoked potential (BAEP) test, implemented by the Audiology Department of the Center for Studies in Occupational Health and Human Ecology (CESTEH) through the application of a questionnaire and to verify whether the SOPs are effective and assess the necessity for improvement. The study was conducted in three phases: in the first phase, eight speech-language pathologists and seven physicians, with no experience in BAEP, were instructed to read and perform each SOP, eventually all individuals evaluated the SOPs by responding to a questionnaire; in the second phase, the questionnaires were analyzed and the three SOP texts were reviewed; in the third phase, nine speech-language pathologists and six physicians, also with no experience in BAEP, read and re-evaluated the reviewed SOPs through a questionnaire. In the first phase, difficulties in understanding the texts were found, raising doubts about the procedures; however, every participant was able to perform the procedure as a whole. In the third phase, after the review, all individuals were able to perform the procedures appropriately and continuously without any doubts. The assessment of the SOPs by questionnaires showed the need for adaptation in the texts. After the texts were reviewed according to the suggestions of the health professionals, it was possible to observe that the SOPs assisted in the execution of the task, which was conducted without any difficulties or doubts, being regarded effective and ensuring quality to the service offered.

  15. Transcriptome analysis of a subtropical deciduous tree: autumn leaf senescence gene expression profile of Formosan gum.

    Science.gov (United States)

    Wen, Chi-Hsiang; Lin, Shih-Shun; Chu, Fang-Hua

    2015-01-01

    Autumn leaf senescence is a spectacular natural phenomenon; however, the regulation networks controlling autumnal colors and the leaf senescence program remain largely unelucidated. Whether regulation of leaf senescence is similar in subtropical deciduous plants and temperate deciduous plants is also unknown. In this study, the gene expression of a subtropical deciduous tree, Formosan gum (Liquidambar formosana Hance), was profiled. The transcriptomes of April leaves (green leaves, 'G') and December leaves (red leaves, 'R') were investigated by next-generation gene sequencing. Out of 58,402 de novo assembled contigs, 32,637 were annotated as putative genes. Furthermore, the L. formosana-specific microarray designed based on total contigs was used to extend the observation period throughout the growing seasons of 2011-2013. Network analysis from the gene expression profile focused on the genes up-regulated when autumn leaf senescence occurred. LfWRKY70, LfWRKY75, LfWRKY65, LfNAC1, LfSPL14, LfNAC100 and LfMYB113 were shown to be key regulators of leaf senescnece, and the genes regulated by LfWRKY75, LfNAC1 and LfMYB113 are candidates to link chlorophyll degradation and anthocyanin biosynthesis to senescence. In summary, the gene expression profiles over the entire year of the developing leaf from subtropical deciduous trees were used for in silico analysis and the putative gene regulation in autumn coloration and leaf senescence is discussed in this study. © The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  16. Profiling physical activity motivation based on self-determination theory: a cluster analysis approach.

    Science.gov (United States)

    Friederichs, Stijn Ah; Bolman, Catherine; Oenema, Anke; Lechner, Lilian

    2015-01-01

    In order to promote physical activity uptake and maintenance in individuals who do not comply with physical activity guidelines, it is important to increase our understanding of physical activity motivation among this group. The present study aimed to examine motivational profiles in a large sample of adults who do not comply with physical activity guidelines. The sample for this study consisted of 2473 individuals (31.4% male; age 44.6 ± 12.9). In order to generate motivational profiles based on motivational regulation, a cluster analysis was conducted. One-way analyses of variance were then used to compare the clusters in terms of demographics, physical activity level, motivation to be active and subjective experience while being active. Three motivational clusters were derived based on motivational regulation scores: a low motivation cluster, a controlled motivation cluster and an autonomous motivation cluster. These clusters differed significantly from each other with respect to physical activity behavior, motivation to be active and subjective experience while being active. Overall, the autonomous motivation cluster displayed more favorable characteristics compared to the other two clusters. The results of this study provide additional support for the importance of autonomous motivation in the context of physical activity behavior. The three derived clusters may be relevant in the context of physical activity interventions as individuals within the different clusters might benefit most from different intervention approaches. In addition, this study shows that cluster analysis is a useful method for differentiating between motivational profiles in large groups of individuals who do not comply with physical activity guidelines.

  17. Association of rs1122608 with Coronary Artery Disease and Lipid Profile: A Meta-analysis.

    Science.gov (United States)

    Liu, Shuo; Xiu, Bingqiu; Liu, Jingdong; Xue, Aimin; Tang, Qiqun; Shen, Yiwen; Xie, Jianhui

    2016-05-01

    It has been reported that rs1122608 adjacent to low-density lipoprotein cholesterol receptor (LDLR) locus is associated with the risk of coronary artery disease (CAD) and blood lipid profile in the Caucasian population. Due to the contradictory results in the Asian population, we conducted a meta-analysis to systematically summarize and clarify the association between rs1122608 with CAD risk and lipid profile. A systematic search regarding studies on the association of rs1122608 with CAD risk and lipid profile was conducted in databases including PubMed, Embase, and Cochrane library. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were used to pool the effect size. A total of five case-control studies were included in this study. A statistically significant association was identified between rs1122608-G allele and CAD risk in overall analysis (OR = 2.09, 95% CI 1.48-2.97) and in both Asian (OR = 1.82, 95% CI 1.04-3.18) and Caucasian subgroups (OR = 2.31, 95% CI 1.48-3.60). The rs1122608-G allele was associated with increased triglyceride (TG) level (OR = 1.25, 95% CI 1.03-1.52), but not with total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), or LDL cholesterol level. Moreover, the rs1122608-G allele is associated with increased CAD risk in the Asian male population (OR = 3.37, 95% CI 1.51-9.86) but not in the Asian female population. The rs1122608 is associated with the risk of CAD and TG level. The rs1122608-G allele was a significant risk factor of CAD in the Asian male population but not in the Asian female population. Copyright © 2016 IMSS. Published by Elsevier Inc. All rights reserved.

  18. Comparative Analysis of Electrophoretic Profile of Major Proteins of Milk from Alpine and Carpathian Goats

    Directory of Open Access Journals (Sweden)

    Alina NASALEAN

    2017-05-01

    Full Text Available The milk’s proteins provide nutritional and biologically active values, essential in human and animal nutrition. In the case of goat milk, the proteins’ concentration and quality represent basic indices for the evaluation of the nutritional and biologically active values. The proposal is to comparatively analyse the protein profile of milk. The milk was collected from two different breeds: French Alpine and Romanian Carpathian. During March and April 2016 there were collected samples of raw milk in hygienic and sanitation conditions. There were two lots: first lot has 10 Carpathian goats and the second lot has 10 Alpine goats. The protein composition of goat milk was established with SDS-PAGE, after the evaluation of the total proteins’ concentration with the Bradford method. The quantitative and percentage data obtained with electrophoresis revealed few differences between those 8 identified protein fractions. Between those two lots, regarding the levels of β-CN, k-CN and β-lactoglobulines there were significant differences. The other protein fractions have values almost identical. Statistical analysis of obtained data shaped the differences in the protein profile at those two breeds. Based on those differences it is to note the superior potential of the Alpine breed regarding the content in biologically active milk proteins. Regarding the obtained data, this study brings new contributions for the evaluation and analysis of protein profile as a nutritive and biologically active component of goat milk, confirming its character as a functional aliment.

  19. Evidence for proposed ICD-11 PTSD and complex PTSD: a latent profile analysis

    Directory of Open Access Journals (Sweden)

    Marylène Cloitre

    2013-05-01

    Full Text Available Background: The WHO International Classification of Diseases, 11th version (ICD-11, has proposed two related diagnoses, posttraumatic stress disorder (PTSD and complex PTSD within the spectrum of trauma and stress-related disorders. Objective: To use latent profile analysis (LPA to determine whether there are classes of individuals that are distinguishable according to the PTSD and complex PTSD symptom profiles and to identify potential differences in the type of stressor and severity of impairment associated with each profile. Method: An LPA and related analyses were conducted on 302 individuals who had sought treatment for interpersonal traumas ranging from chronic trauma (e.g., childhood abuse to single-incident events (e.g., exposure to 9/11 attacks. Results: The LPA revealed three classes of individuals: (1 a complex PTSD class defined by elevated PTSD symptoms as well as disturbances in three domains of self-organization: affective dysregulation, negative self-concept, and interpersonal problems; (2 a PTSD class defined by elevated PTSD symptoms but low scores on the three self-organization symptom domains; and (3 a low symptom class defined by low scores on all symptoms and problems. Chronic trauma was more strongly predictive of complex PTSD than PTSD and, conversely, single-event trauma was more strongly predictive of PTSD. In addition, complex PTSD was associated with greater impairment than PTSD. The LPA analysis was completed both with and without individuals with borderline personality disorder (BPD yielding identical results, suggesting the stability of these classes regardless of BPD comorbidity. Conclusion: Preliminary data support the proposed ICD-11 distinction between PTSD and complex PTSD and support the value of testing the clinical utility of this distinction in field trials. Replication of results is necessary.For the abstract or full text in other languages, please see Supplementary files under Article Tools online

  20. Sesame fractions and lipid profiles: a systematic review and meta-analysis of controlled trials.

    Science.gov (United States)

    Khalesi, Saman; Paukste, Ernesta; Nikbakht, Elham; Khosravi-Boroujeni, Hossein

    2016-03-14

    Increased plasma lipid profiles are among the most important risk factors of CHD and stroke. Sesame contains considerable amounts of vitamin E, MUFA, fibre and lignans, which are thought to be associated with its plasma lipid-lowering properties. This study aimed to systematically review the evidence and identify the effects of sesame consumption on blood lipid profiles using a meta-analysis of controlled trials. PubMed, CINAHL and Cochrane Library databases were searched (from 1960 to May 2015). A total of ten controlled trials were identified based on the eligibility criteria. Both the Cochrane Collaboration tool and the Rosendal scale were used to assess the risk of bias of the included studies. The meta-analysis results showed that consumption of sesame did not significantly change the concentrations of total blood cholesterol (-0·32 mmol/l; 95% CI -0·75, 0·11; P=0·14, I(2)=96%), LDL-cholesterol (-0·15 mmol/l; 95% CI -0·50, 0·19; P=0·39, I(2)=96%) or HDL-cholesterol (0·01 mmol/l; 95% CI -0·00, 0·02; P=0·16, I(2)=0%). However, a significant reduction was observed in serum TAG levels (-0·24 mmol/l; 95% CI -0·32, -0·15; Psesame. It was concluded that sesame consumption can significantly reduce blood TAG levels but there is insufficient evidence to support its hypocholesterolaemic effects. Further studies are required to determine the potential effect of sesame consumption on lipid profiles and cardiovascular risk factors.

  1. Visualizing nD Point Clouds as Topological Landscape Profiles to Guide Local Data Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Oesterling, Patrick [Univ. of Leipzig (Germany). Computer Science Dept.; Heine, Christian [Univ. of Leipzig (Germany). Computer Science Dept.; Federal Inst. of Technology (ETH), Zurich (Switzerland). Dept. of Computer Science; Weber, Gunther H. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Computational Research Division; Scheuermann, Gerik [Univ. of Leipzig (Germany). Computer Science Dept.

    2012-05-04

    Analyzing high-dimensional point clouds is a classical challenge in visual analytics. Traditional techniques, such as projections or axis-based techniques, suffer from projection artifacts, occlusion, and visual complexity.We propose to split data analysis into two parts to address these shortcomings. First, a structural overview phase abstracts data by its density distribution. This phase performs topological analysis to support accurate and non-overlapping presentation of the high-dimensional cluster structure as a topological landscape profile. Utilizing a landscape metaphor, it presents clusters and their nesting as hills whose height, width, and shape reflect cluster coherence, size, and stability, respectively. A second local analysis phase utilizes this global structural knowledge to select individual clusters or point sets for further, localized data analysis. Focusing on structural entities significantly reduces visual clutter in established geometric visualizations and permits a clearer, more thorough data analysis. In conclusion, this analysis complements the global topological perspective and enables the user to study subspaces or geometric properties, such as shape.

  2. Using CV-GLUE procedure in analysis of wetland model predictive uncertainty.

    Science.gov (United States)

    Huang, Chun-Wei; Lin, Yu-Pin; Chiang, Li-Chi; Wang, Yung-Chieh

    2014-07-01

    This study develops a procedure that is related to Generalized Likelihood Uncertainty Estimation (GLUE), called the CV-GLUE procedure, for assessing the predictive uncertainty that is associated with different model structures with varying degrees of complexity. The proposed procedure comprises model calibration, validation, and predictive uncertainty estimation in terms of a characteristic coefficient of variation (characteristic CV). The procedure first performed two-stage Monte-Carlo simulations to ensure predictive accuracy by obtaining behavior parameter sets, and then the estimation of CV-values of the model outcomes, which represent the predictive uncertainties for a model structure of interest with its associated behavior parameter sets. Three commonly used wetland models (the first-order K-C model, the plug flow with dispersion model, and the Wetland Water Quality Model; WWQM) were compared based on data that were collected from a free water surface constructed wetland with paddy cultivation in Taipei, Taiwan. The results show that the first-order K-C model, which is simpler than the other two models, has greater predictive uncertainty. This finding shows that predictive uncertainty does not necessarily increase with the complexity of the model structure because in this case, the more simplistic representation (first-order K-C model) of reality results in a higher uncertainty in the prediction made by the model. The CV-GLUE procedure is suggested to be a useful tool not only for designing constructed wetlands but also for other aspects of environmental management. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. MMPI-2: Cluster Analysis of Personality Profiles in Perinatal Depression—Preliminary Evidence

    Directory of Open Access Journals (Sweden)

    Valentina Meuti

    2014-01-01

    Full Text Available Background. To assess personality characteristics of women who develop perinatal depression. Methods. The study started with a screening of a sample of 453 women in their third trimester of pregnancy, to which was administered a survey data form, the Edinburgh Postnatal Depression Scale (EPDS and the Minnesota Multiphasic Personality Inventory 2 (MMPI-2. A clinical group of subjects with perinatal depression (PND, 55 subjects was selected; clinical and validity scales of MMPI-2 were used as predictors in hierarchical cluster analysis carried out. Results. The analysis identified three clusters of personality profile: two “clinical” clusters (1 and 3 and an “apparently common” one (cluster 2. The first cluster (39.5% collects structures of personality with prevalent obsessive or dependent functioning tending to develop a “psychasthenic” depression; the third cluster (13.95% includes women with prevalent borderline functioning tending to develop “dysphoric” depression; the second cluster (46.5% shows a normal profile with a “defensive” attitude, probably due to the presence of defense mechanisms or to the fear of stigma. Conclusion. Characteristics of personality have a key role in clinical manifestations of perinatal depression; it is important to detect them to identify mothers at risk and to plan targeted therapeutic interventions.

  4. Global expression profiling applied to the analysis of Arabidopsis stamen development.

    Science.gov (United States)

    Alves-Ferreira, Márcio; Wellmer, Frank; Banhara, Aline; Kumar, Vijaya; Riechmann, José Luis; Meyerowitz, Elliot M

    2007-11-01

    To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.

  5. Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development1[W][OA

    Science.gov (United States)

    Alves-Ferreira, Márcio; Wellmer, Frank; Banhara, Aline; Kumar, Vijaya; Riechmann, José Luis; Meyerowitz, Elliot M.

    2007-01-01

    To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase. PMID:17905860

  6. Rasch Analysis of the Profiles of Occupational Engagement in people with Severe mental illness (POES) instrument.

    Science.gov (United States)

    Bejerholm, Ulrika; Lundgren-Nilsson, Åsa

    2015-08-19

    The Profiles of Occupational Engagement in people with Severe mental illness (POES) instrument was developed to study time use profiles of occupations and measure the extent they are characterized by engagement. However, the dimensional factors are not known. The aim of the present study was to establish the internal construct validity of the POES using the Rasch measurement model. A sample of 192 outpatients in Sweden was administered the POES and data were subjected to Rasch analysis. The POES showed good fit to the Rasch model after accommodation for local dependency. The nine items had high reliability as measured by person separation index, and no threshold disordering was present. Differential item functioning analysis showed no significant differences across groups of age, sex, diagnosis, or country of origin. The POES is a unidimensional scale that represents a continuum of occupational engagement. The transformed POES sum score can be used on an interval scale to measure status and changes in occupational engagement in mental health practice and research.

  7. Principal component analysis of proteolytic profiles as markers of authenticity of PDO cheeses.

    Science.gov (United States)

    Guerreiro, Joana Santos; Barros, Mário; Fernandes, Paulo; Pires, Preciosa; Bardsley, Ronald

    2013-02-15

    The casein fraction of 13 Portuguese PDO cheeses were analysed using Urea-PAGE and reverse phase-high performance liquid chromatography (RP-HPLC) and then subjected to chemometric evaluation. The chemometric techniques of cluster analysis (CA) and principal component analysis (PCA) were used for the classification studies. Peptide mapping using Urea-PAGE followed by CA revealed two major clusters according to the similarity of the proteolytic profile of the cheeses. PCA results were in accordance with the grouping performed using CA. CA of RP-HPLC results of the matured cheeses revealed the presence of one major cluster comprising samples manufactured with only ovine milk or milk admixtures. When the results of CA technique were compared with the two PCA approaches performed, it was found that the grouping of the samples was similar. Both approaches, revealed the potential of proteolytic profiles (which is an essential aspect of cheese maturation) as markers of authenticity of PDO cheeses in terms of ripening time and milk admixtures not mentioned on the label. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. NanoOK: multi-reference alignment analysis of nanopore sequencing data, quality and error profiles.

    Science.gov (United States)

    Leggett, Richard M; Heavens, Darren; Caccamo, Mario; Clark, Matthew D; Davey, Robert P

    2016-01-01

    The Oxford Nanopore MinION sequencer, currently in pre-release testing through the MinION Access Programme (MAP), promises long reads in real-time from an inexpensive, compact, USB device. Tools have been released to extract FASTA/Q from the MinION base calling output and to provide basic yield statistics. However, no single tool yet exists to provide comprehensive alignment-based quality control and error profile analysis--something that is extremely important given the speed with which the platform is evolving. NanoOK generates detailed tabular and graphical output plus an in-depth multi-page PDF report including error profile, quality and yield data. NanoOK is multi-reference, enabling detailed analysis of metagenomic or multiplexed samples. Four popular Nanopore aligners are supported and it is easily extensible to include others. NanoOK is an open-source software, implemented in Java with supporting R scripts. It has been tested on Linux and Mac OS X and can be downloaded from https://github.com/TGAC/NanoOK. A VirtualBox VM containing all dependencies and the DH10B read set used in this article is available from http://opendata.tgac.ac.uk/nanook/. A Docker image is also available from Docker Hub--see program documentation https://documentation.tgac.ac.uk/display/NANOOK. richard.leggett@tgac.ac.uk Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press.

  9. Latent profile analysis and comorbidity in a sample of individuals with compulsive buying disorder.

    Science.gov (United States)

    Mueller, Astrid; Mitchell, James E; Black, Donald W; Crosby, Ross D; Berg, Kelly; de Zwaan, Martina

    2010-07-30

    The aims of this study were to perform a latent profile analysis in a sample of individuals with compulsive buying, to explore the psychiatric comorbidity, and to examine whether or not more severe compulsive buying is associated with greater comorbidity. Compulsive buying measures and SCID data obtained from 171 patients with compulsive buying behavior who had participated in treatment trials at different clinical centers in the U.S. and Germany were analyzed. Latent profile analysis produced two clusters. Overall, cluster 2, included subjects with more severe compulsive buying, and was characterized by higher lifetime as well as current prevalence rates for Axis I and impulse control disorders. Nearly 90% of the total sample reported at least one lifetime Axis I diagnosis, particularly mood (74%) and anxiety (57%) disorders. Twenty-one percent had a comorbid impulse control disorder, most commonly intermittent explosive disorder (11%). Half of the sample presented with at least one current Axis I disorder, most commonly anxiety disorders (44%). Given the substantial psychiatric comorbidity, it is reasonable to question whether or not compulsive buying represents a distinct psychiatric entity vs. an epiphenomenon of other psychiatric disorders. Copyright 2010 Elsevier Ltd. All rights reserved.

  10. Transcriptome analysis reveals non-identical microRNA profiles between arterial and venous plasma

    Science.gov (United States)

    Xu, Guoheng; Geng, Bin; Cui, Qinghua

    2017-01-01

    Circulating microRNAs presented in venous plasma have been demonstrated as powerful biomarkers for the complex diseases like cancer. Nevertheless, those presented in arterial plasma remained largely unexplored. Here, using microarray technique, we compared microRNA expression profiles of the matched arterial and venous plasma samples from the same male rats. Though the microRNA profiles were largely similar, we identified 24 differentially expressed microRNAs, including 10 arterial highly expressed microRNAs and 14 venous highly expressed microRNAs. The differentially expressed microRNAs were validated by qRT-PCR. Computational analysis of these microRNAs and their targets indicated that arterial highly expressed microRNAs were overrepresented for functional terms like hematopoiesis and diseases like Crohn's Disease and leukemia; while venous highly expressed microRNAs were enriched for cell differentiation function, and diseases like distal myopathies and heart failure. Our analysis also suggested significant correlations between plasma microRNA expression and tissue microRNA expression. Four arterial highly expressed microRNAs also showed enriched expression in specific tissues and would be novel biomarker candidates. PMID:28212530

  11. Proteomic analysis of plasma profiles in children with recurrent bone fractures.

    Science.gov (United States)

    Rusińska, Agnieszka; Świątkowska, Maria; Koziołkiewicz, Wiktor; Skurzyński, Szymon; Golec, Joanna; Chlebna-Sokół, Danuta

    2011-01-01

    The aim of the study is proteomic analysis of the plasma profile in children with recurrent bone fractures. The study involved 16 children: 6 patients with recurrent low-energy fractures and normal bone mass and 10 with osteogenesis imperfecta. In the analysis of the protein profile, the two-dimensional protein electrophoresis was used (Ettan DALT II, Amersham Bioscience). The images of protein gels were compared with controls. The protein spots with changed expression were cut from the gel and the amino acid sequence was analyzed with the mass spectrometry method (Q-Tof Premier(TM) API MASS SPECTROMETR, Waters) for protein identification. The most prevalent protein with changed expression, with respect to controls, was haptoglobin observed in 6 patients with a severe form of osteogenesis imperfecta. Increased haptoglobin concentration in these patients was confirmed by the ELISA method. Peptides corresponding to alpha-1 acid glycoprotein and serum amyloid P-component, apolipoprotein A-I, and transthyretin were detected in one, two and three children, respectively. 1) The results show increased haptoglobin which may be suggestive of an inflammatory component taking part in the course of osteogenesis imperfecta. 2) Further studies to explain the possible relationship of this protein with increased bone fragility are necessary.

  12. Tissue specific profiling of females of Schistosoma japonicum by integrated laser microdissection microscopy and microarray analysis.

    Directory of Open Access Journals (Sweden)

    Geoffrey N Gobert

    2009-06-01

    Full Text Available The functions of many schistosome gene products remain to be characterized. A major step towards elucidating function of these genes would be in defining their sites of expression. This goal is rendered difficult to achieve by the generally small size of the parasites and the lack of a body cavity, which precludes analysis of transcriptional profiles of the tissues in isolation.Here, we describe a combined laser microdissection microscopy (LMM and microarray analysis approach to expedite tissue specific profiling and gene atlasing for tissues of adult female Schistosoma japonicum. This approach helps to solve the gene characterization "bottle-neck" brought about by acoelomy and the size of these parasites. Complementary RNA obtained after isolation from gastrodermis (parasite gut mucosa, vitelline glands and ovary by LMM were subjected to microarray analyses, resulting in identification of 147 genes upregulated in the gastrodermis, 4,149 genes in the ovary and 2,553 in the vitellaria.This work will help to shed light on the molecular pathobiology of this debilitating human parasite and aid in the discovery of new targets for the development of anti-schistosome vaccines and drugs.

  13. Latent profile analysis in frontotemporal lobar degeneration and related disorders: clinical presentation and SPECT functional correlates

    Directory of Open Access Journals (Sweden)

    Di Luca Monica

    2007-05-01

    Full Text Available Abstract Background Frontotemporal Lobar Degeneration (FTLD thus recently renamed, refers to a spectrum of heterogeneous conditions. This same heterogeneity of presentation represents the major methodological limit for the correct evaluation of clinical designation and brain functional correlates. At present, no study has investigated clinical clusters due to specific cognitive and behavioural disturbances beyond current clinical criteria. The aim of this study was to identify clinical FTLD presentation, based on cognitive and behavioural profile, and to define their SPECT functional correlations. Methods Ninety-seven FTLD patients entered the study. A clinical evaluation and standardised assessment were preformed, as well as a brain SPECT perfusion imaging study. Latent Profile Analysis on clinical, neuropsychological, and behavioural data was performed. Voxel-basis analysis of SPECT data was computed. Results Three specific clusters were identified and named "pseudomanic behaviour" (LC1, "cognitive" (LC2, and "pseudodepressed behaviour" (LC3 endophenotypes. These endophenotypes showed a comparable hypoperfusion in left temporal lobe, but a specific pattern involving: medial and orbitobasal frontal cortex in LC1, subcortical brain region in LC2, and right dorsolateral frontal cortex and insula in LC3. Conclusion These findings provide evidence that specific functional-cluster symptom relationship can be delineated in FTLD patients by a standardised assessment. The understanding of the different functional correlates of clinical presentations will hopefully lead to the possibility of individuating diagnostic and treatment algorithms.

  14. Translatome profiling: methods for genome-scale analysis of mRNA translation.

    Science.gov (United States)

    King, Helen A; Gerber, André P

    2016-01-01

    During the past decade, there has been a rapidly increased appreciation of the role of translation as a key regulatory node in gene expression. Thereby, the development of methods to infer the translatome, which refers to the entirety of mRNAs associated with ribosomes for protein synthesis, has facilitated the discovery of new principles and mechanisms of translation and expanded our view of the underlying logic of protein synthesis. Here, we review the three main methodologies for translatome analysis, and we highlight some of the recent discoveries made using each technique. We first discuss polysomal profiling, a classical technique that involves the separation of mRNAs depending on the number of bound ribosomes using a sucrose gradient, and which has been combined with global analysis tools such as DNA microarrays or high-throughput RNA sequencing to identify the RNAs in polysomal fractions. We then introduce ribosomal profiling, a recently established technique that enables the mapping of ribosomes along mRNAs at near-nucleotide resolution on a global scale. We finally refer to ribosome affinity purification techniques that are based on the cell-type-specific expression of tagged ribosomal proteins, allowing the capture of translatomes from specialized cells in organisms. We discuss the advantages and disadvantages of these three main techniques in the pursuit of defining the translatome, and we speculate about future developments. © The Author 2014. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  15. Authentication of Zanthoxylum Species Based on Integrated Analysis of Complete Chloroplast Genome Sequences and Metabolite Profiles.

    Science.gov (United States)

    Lee, Hyeon Ju; Koo, Hyun Jo; Lee, Jonghoon; Lee, Sang-Choon; Lee, Dong Young; Giang, Vo Ngoc Linh; Kim, Minjung; Shim, Hyeonah; Park, Jee Young; Yoo, Ki-Oug; Sung, Sang Hyun; Yang, Tae-Jin

    2017-11-29

    We performed chloroplast genome sequencing and comparative analysis of two Rutaceae species, Zanthoxylum schinifolium (Korean pepper tree) and Z. piperitum (Japanese pepper tree), which are medicinal and culinary crops in Asia. We identified more than 837 single nucleotide polymorphisms and 103 insertions/deletions (InDels) based on a comparison of the two chloroplast genomes and developed seven DNA markers derived from five tandem repeats and two InDel variations that discriminated between Korean Zanthoxylum species. Metabolite profile analysis pointed to three metabolic groups, one with Korean Z. piperitum samples, one with Korean Z. schinifolium samples, and the last containing all the tested Chinese Zanthoxylum species samples, which are considered to be Z. bungeanum based on our results. Two markers were capable of distinguishing among these three groups. The chloroplast genome sequences identified in this study represent a valuable genomics resource for exploring diversity in Rutaceae, and the molecular markers will be useful for authenticating dried Zanthoxylum berries in the marketplace.

  16. Aromatic profile of ciders by chemical quantitative, gas chromatography-olfactometry, and sensory analysis.

    Science.gov (United States)

    Antón, María José; Suárez Valles, Belén; García Hevia, Ana; Picinelli Lobo, Anna

    2014-01-01

    Nine samples of Asturias cider have been analyzed for volatile, olfactometric, and sensorial profiles. The aromatic composition was mainly constituted by fusel alcohols and ethyl esters. Among the minor volatile compounds, fatty acids, volatile phenols, and alcohols were the main components. The olfactometric analysis revealed the existence of 55 aromatic areas, exhibiting a wide range of intensities. Components like amyl alcohols, 2-phenylethanol, ethyl esters such as 2-methylbutyrate, hexanoate and octanoate, hexanoic and octanoic acids 2-phenylethyl acetate, 4-ethyl guaiacol, and 4-ethyl phenol could be considered as being part of the structure of cider aroma. The extract dilution analysis of one extract identified 2 volatile phenols (4-ethyl guaiacol and 4-ethyl phenol) among the most powerful odorants in cider. These components gave significant correlations with the sensory attributes sweet, spicy, and lees. © 2013 Institute of Food Technologists®

  17. Multi-elemental profile of some Brazilian make-up products by instrumental neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Dalmazio, Ilza; Menezes, Maria Angela de B.C., E-mail: id@cdtn.b, E-mail: menezes@cdtn.b [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Servico de Reator e Tecnicas Analiticas. Lab. de Ativacao Neutronica

    2011-07-01

    Recent works have shown that analysis in cosmetics and beauty products from the European and Asian markets indicate the presence of U, Th and rare earths besides other trace elements. Considering these previous findings and health issues, it would be valuable to obtain information on elements in cosmetics available in the Brazilian market. The purpose of this study was to acquire a multi-elemental profile of some Brazilian make-up products of diverse brands. Samples of eye shadow, liquid base, facial concealer, lipstick, and compact face powder were analyzed applying neutron activation analysis, k{sub 0}-standardization method at CDTN/CNEN, using the TRIGA Mark I IPR-R1 research reactor. Concentrations of more than 30 elements in samples are presented and it was found elements included in Brazilian National Health Surveillance Agency prohibitive list, rare earths, Th and U in a minimum of two cosmetic samples. (author)

  18. Quantitative Analysis of Human Pluripotency and Neural Specification by In-Depth (PhosphoProteomic Profiling

    Directory of Open Access Journals (Sweden)

    Ilyas Singec

    2016-09-01

    Full Text Available Controlled differentiation of human embryonic stem cells (hESCs can be utilized for precise analysis of cell type identities during early development. We established a highly efficient neural induction strategy and an improved analytical platform, and determined proteomic and phosphoproteomic profiles of hESCs and their specified multipotent neural stem cell derivatives (hNSCs. This quantitative dataset (nearly 13,000 proteins and 60,000 phosphorylation sites provides unique molecular insights into pluripotency and neural lineage entry. Systems-level comparative analysis of proteins (e.g., transcription factors, epigenetic regulators, kinase families, phosphorylation sites, and numerous biological pathways allowed the identification of distinct signatures in pluripotent and multipotent cells. Furthermore, as predicted by the dataset, we functionally validated an autocrine/paracrine mechanism by demonstrating that the secreted protein midkine is a regulator of neural specification. This resource is freely available to the scientific community, including a searchable website, PluriProt.

  19. An investigation into the sample preparation procedure and analysis of cyanoacrylate adhesives using capillary electrophoresis

    OpenAIRE

    Whitaker, Gillian; Kincaid, Brendan J.; Van Hoof, Nicole; Regan, Fiona; Smyth, Malcolm R.; Leonard, Raymond G.

    2007-01-01

    In this study, the trace acid profile of cyanoacrylate adhesives was studied using capillary electrophoresis. Liquid–liquid extraction was employed as the sample preparation step before separation by capillary electrophoresis. The solubility of the adhesives was investigated using various organic solvents, e.g. hexane and dichloromethane, and chloroform was determined to be the optimum solvent as it enabled the full dissolution of the adhesive. A comprehensive stability study was performed ov...

  20. Single molecule FRET data analysis procedures for FRET efficiency determination: probing the conformations of nucleic acid structures.

    Science.gov (United States)

    Krüger, Asger Christian; Birkedal, Victoria

    2013-11-01

    Single molecule FRET microscopy is an attractive technique for studying structural dynamics and conformational diversity of nucleic acid structures. Some of its strengths are that it can follow structural changes on a fast time scale and identify conformation distributions arising from dynamic or static population heterogeneity. Here, we give a description of the experiment and data analysis procedures of this method and detail what parameters are needed for FRET efficiency calculation. Using single molecule FRET data obtained on G-quadruplex DNA structures that exhibit large conformation diversity, we illustrate that the shape of the FRET distribution changes depending on what parameters are included in the data analysis procedure. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. Genome-wide transcriptome and expression profile analysis of Phalaenopsis during explant browning.

    Directory of Open Access Journals (Sweden)

    Chuanjun Xu

    Full Text Available Explant browning presents a major problem for in vitro culture, and can lead to the death of the explant and failure of regeneration. Considerable work has examined the physiological mechanisms underlying Phalaenopsis leaf explant browning, but the molecular mechanisms of browning remain elusive. In this study, we used whole genome RNA sequencing to examine Phalaenopsis leaf explant browning at genome-wide level.We first used Illumina high-throughput technology to sequence the transcriptome of Phalaenopsis and then performed de novo transcriptome assembly. We assembled 79,434,350 clean reads into 31,708 isogenes and generated 26,565 annotated unigenes. We assigned Gene Ontology (GO terms, Kyoto Encyclopedia of Genes and Genomes (KEGG annotations, and potential Pfam domains to each transcript. Using the transcriptome data as a reference, we next analyzed the differential gene expression of explants cultured for 0, 3, and 6 d, respectively. We then identified differentially expressed genes (DEGs before and after Phalaenopsis explant browning. We also performed GO, KEGG functional enrichment and Pfam analysis of all DEGs. Finally, we selected 11 genes for quantitative real-time PCR (qPCR analysis to confirm the expression profile analysis.Here, we report the first comprehensive analysis of transcriptome and expression profiles during Phalaenopsis explant browning. Our results suggest that Phalaenopsis explant browning may be due in part to gene expression changes that affect the secondary metabolism, such as: phenylpropanoid pathway and flavonoid biosynthesis. Genes involved in photosynthesis and ATPase activity have been found to be changed at transcription level; these changes may perturb energy metabolism and thus lead to the decay of plant cells and tissues. This study provides comprehensive gene expression data for Phalaenopsis browning. Our data constitute an important resource for further functional studies to prevent explant browning.

  2. Global analysis of transcriptome responses and gene expression profiles to cold stress of Jatropha curcas L.

    Science.gov (United States)

    Wang, Haibo; Zou, Zhurong; Wang, Shasha; Gong, Ming

    2013-01-01

    Jatropha curcas L., also called the Physic nut, is an oil-rich shrub with multiple uses, including biodiesel production, and is currently exploited as a renewable energy resource in many countries. Nevertheless, because of its origin from the tropical MidAmerican zone, J. curcas confers an inherent but undesirable characteristic (low cold resistance) that may seriously restrict its large-scale popularization. This adaptive flaw can be genetically improved by elucidating the mechanisms underlying plant tolerance to cold temperatures. The newly developed Illumina Hiseq™ 2000 RNA-seq and Digital Gene Expression (DGE) are deep high-throughput approaches for gene expression analysis at the transcriptome level, using which we carefully investigated the gene expression profiles in response to cold stress to gain insight into the molecular mechanisms of cold response in J. curcas. In total, 45,251 unigenes were obtained by assembly of clean data generated by RNA-seq analysis of the J. curcas transcriptome. A total of 33,363 and 912 complete or partial coding sequences (CDSs) were determined by protein database alignments and ESTScan prediction, respectively. Among these unigenes, more than 41.52% were involved in approximately 128 known metabolic or signaling pathways, and 4,185 were possibly associated with cold resistance. DGE analysis was used to assess the changes in gene expression when exposed to cold condition (12°C) for 12, 24, and 48 h. The results showed that 3,178 genes were significantly upregulated and 1,244 were downregulated under cold stress. These genes were then functionally annotated based on the transcriptome data from RNA-seq analysis. This study provides a global view of transcriptome response and gene expression profiling of J. curcas in response to cold stress. The results can help improve our current understanding of the mechanisms underlying plant cold resistance and favor the screening of crucial genes for genetically enhancing cold resistance

  3. Bioinformatics microarray analysis and identification of gene expression profiles associated with cirrhotic liver

    Directory of Open Access Journals (Sweden)

    Kun-Ming Chan

    2016-04-01

    Full Text Available Cirrhosis is the endpoint of liver fibrosis that is accompanied by limited regeneration capacity and complications and is the ultimate cause of death in many patients. Despite this, few studies have thoroughly looked at the gene expression profiles in the cirrhotic liver. Hence, this study aims to identify the genes that were differentially expressed in the cirrhotic liver and to explore the putative related signaling pathway and interaction networks. The gene expression profiles of cirrhotic livers and noncirrhotic livers were examined and compared using microarray gene analysis. Proteins encoded by the differentially expressed genes were analyzed for functional clustering and signaling pathway involvement using MetaCore bioinformatics analyses. The Gene Ontology analysis as well as the Kyoto encyclopedia of Genes and Genomes pathway analysis were also performed. A total of 213 significant genes were differentially expressed at more than a two-fold change in cirrhotic livers as compared to noncirrhotic livers. Of these, 105 upregulated genes and 63 downregulated genes were validated through MetaCore bioinformatics analyses. The signaling pathways and major functions of proteins encoded by these differentially expressed genes were further analyzed; results showed that the cirrhotic liver has a unique gene expression pattern related to inflammatory reaction, immune response, and cell growth, and is potentially cancer related. Our findings suggest that the microarray analysis may provide clues to the molecular mechanisms of liver cirrhosis for future experimental studies. However, further exploration of areas regarding therapeutic strategy might be possible to support metabolic activity, decrease inflammation, or enhance regeneration for liver cirrhosis.

  4. Global analysis of transcriptome responses and gene expression profiles to cold stress of Jatropha curcas L.

    Directory of Open Access Journals (Sweden)

    Haibo Wang

    Full Text Available BACKGROUND: Jatropha curcas L., also called the Physic nut, is an oil-rich shrub with multiple uses, including biodiesel production, and is currently exploited as a renewable energy resource in many countries. Nevertheless, because of its origin from the tropical MidAmerican zone, J. curcas confers an inherent but undesirable characteristic (low cold resistance that may seriously restrict its large-scale popularization. This adaptive flaw can be genetically improved by elucidating the mechanisms underlying plant tolerance to cold temperatures. The newly developed Illumina Hiseq™ 2000 RNA-seq and Digital Gene Expression (DGE are deep high-throughput approaches for gene expression analysis at the transcriptome level, using which we carefully investigated the gene expression profiles in response to cold stress to gain insight into the molecular mechanisms of cold response in J. curcas. RESULTS: In total, 45,251 unigenes were obtained by assembly of clean data generated by RNA-seq analysis of the J. curcas transcriptome. A total of 33,363 and 912 complete or partial coding sequences (CDSs were determined by protein database alignments and ESTScan prediction, respectively. Among these unigenes, more than 41.52% were involved in approximately 128 known metabolic or signaling pathways, and 4,185 were possibly associated with cold resistance. DGE analysis was used to assess the changes in gene expression when exposed to cold condition (12°C for 12, 24, and 48 h. The results showed that 3,178 genes were significantly upregulated and 1,244 were downregulated under cold stress. These genes were then functionally annotated based on the transcriptome data from RNA-seq analysis. CONCLUSIONS: This study provides a global view of transcriptome response and gene expression profiling of J. curcas in response to cold stress. The results can help improve our current understanding of the mechanisms underlying plant cold resistance and favor the screening of

  5. Content Analysis of Vomit and Diarrhea Cleanup Procedures To Prevent Norovirus Infections in Retail and Food Service Operations.

    Science.gov (United States)

    Chao, Morgan G; Dubé, Anne-Julie; Leone, Cortney M; Moore, Christina M; Fraser, Angela M

    2016-11-01

    Human noroviruses are the leading cause of foodborne disease in the United States, sickening 19 to 21 million Americans each year. Vomit and diarrhea are both highly concentrated sources of norovirus particles. For this reason, establishing appropriate cleanup procedures for these two substances is critical. Food service establishments in states that have adopted the 2009 or 2013 U.S. Food and Drug Administration Food Code are required to have a program detailing specific cleanup procedures. The aim of our study was to determine the alignment of existing vomit and diarrhea cleanup procedures with the 11 elements recommended in Annex 3 of the 2011 Supplement to the 2009 Food Code and to determine their readability and clarity of presentation. In July 2015, we located vomit and diarrhea cleanup procedures by asking Norovirus Collaborative for Outreach, Research, and Education stakeholders for procedures used by their constituency groups and by conducting a Google Advanced Search of the World Wide Web. We performed content analysis to determine alignment with the recommendations in Annex 3. Readability and clarity of presentation were also assessed. A total of 38 artifacts were analyzed. The mean alignment score was 7.0 ± 1.7 of 11 points; the mean clarity score was 6.7 ± 2.5 of 17 points. Only nine artifacts were classified as high clarity, high alignment. Vomit and diarrhea cleanup procedures should align with Annex 3 in the Food Code and should, as well, be clearly presented; yet, none of the artifacts completely met both conditions. To reduce the spread of norovirus infections in food service establishments, editable guidelines are needed that are aligned with Annex 3 and are clearly written, into which authors could insert their facility-specific information.

  6. Transcranial electrical stimulation during sleep enhances declarative (but not procedural) memory consolidation: Evidence from a meta-analysis.

    Science.gov (United States)

    Barham, Michael P; Enticott, Peter G; Conduit, Russell; Lum, Jarrad A G

    2016-04-01

    This meta-analysis summarizes research examining whether transcranial electrical stimulation (transcranial direct current stimulation with oscillating and constant currents; transcranial alternating current stimulation), administered during sleep, can modulate declarative and procedural memory consolidation. Included in the meta-analysis were 13 experiments that represented data from 179 participants. Study findings were summarized using standardized mean difference (SMD) which is an effect size that summarizes differences in standard deviation units. Results showed electrical stimulation during sleep could enhance (SMD=0.447; p=.003) or disrupt (SMD=-0.476, p=.030) declarative memory consolidation. However, transcranial electric stimulation does not appear to be able to enhance (SMD=0.154, p=.279) or disrupt (SMD=0.076, p=.675) procedural memory consolidation. This meta-analysis provides strong evidence that TES is able to modulate some consolidation processes. Additional research is required to determine the mechanisms by which transcranial electrical stimulation is able to influence declarative memory consolidation. Finally, it is yet to be determined whether transcranial electrical stimulation can modulate procedural memory consolidation. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

  7. Interphase Chromosome Profiling: A Method for Conventional Banded Chromosome Analysis Using Interphase Nuclei.

    Science.gov (United States)

    Babu, Ramesh; Van Dyke, Daniel L; Dev, Vaithilingam G; Koduru, Prasad; Rao, Nagesh; Mitter, Navnit S; Liu, Mingya; Fuentes, Ernesto; Fuentes, Sarah; Papa, Stephen

    2017-10-05

    - Chromosome analysis on bone marrow or peripheral blood samples fails in a small proportion of attempts. A method that is more reliable, with similar or better resolution, would be a welcome addition to the armamentarium of the cytogenetics laboratory. - To develop a method similar to banded metaphase chromosome analysis that relies only on interphase nuclei. - To label multiple targets in an equidistant fashion along the entire length of each chromosome, including landmark subtelomere and centromere regions. Each label so generated by using cloned bacterial artificial chromosome probes is molecularly distinct with unique spectral characteristics, so the number and position of the labels can be tracked to identify chromosome abnormalities. - Interphase chromosome profiling (ICP) demonstrated results similar to conventional chromosome analysis and fluorescence in situ hybridization in 55 previously studied cases and obtained useful ICP chromosome analysis results on another 29 cases in which conventional methods failed. - ICP is a new and powerful method to karyotype peripheral blood and bone marrow aspirate preparations without reliance on metaphase chromosome preparations. It will be of particular value for cases with a failed conventional analysis or when a fast turnaround time is required.

  8. A test procedure for energetic and performance analysis of cold appliances for the food industry

    Science.gov (United States)

    Armani, F.; Boscolo, A.

    2013-09-01

    In this article we present a novel approach for the characterization of cold appliances and in particular of refrigerators based on the standard vapour compression cycle with a reciprocating on/off compressor. The test procedure is based on a virtual instrument that perform both the stimulus and the data acquisition on the device under test. Acquired data is elaborated to fit a semi-empirical model based on the energetic balances between thermal and electrical sub systems and the heat exchanged with the environment. This approach results in a simple method to calculate useful parameters of the refrigerator, such as energetic performance, cooling effect and limit values of thermal loads. The test procedure requires only a few temperatures and the electric power consumption to be monitored, resulting in a low impact on the refrigerator. Preliminary tests showed a good estimation of parameters and prediction of energy consumption and heat extraction capacity of the refrigerator under test.

  9. Analysis of holographic interferograms of the expanded cornea after refractive surgery procedure

    Science.gov (United States)

    Kasprzak, Henryk T.; Jaronski, Jaroslaw W.; Foerster, Werner; von Bally, Gert

    1994-12-01

    The paper presents results of holographic experiments of expanding bovine cornea in vitro, after different refractive surgery procedure (keratoplasty). The corneas of fresh, enucleated bovine eyes were subjected to different refractive procedures, such as: radial and tangential incisions, mechanical ablation of the corneal layers as well as ablation by means of excimer laser. After keratoplasty, the eyeball was placed into the holographic set-up and the needle connected to a pressure meter was inserted into the anterior chamber of the eye. Double exposure, as well as real time holographic interferograms of expanding corneas were recorded by means of photothermoplastic camera, due to small intraocular pressure differences in order of a few Pa. The holographic interferograms were stored and processed in the computer memory by use of CCD camera and the image processing board. Quantitative results are presented in the form of a 3-D surface plot of the displacement vectors of the corneal surface and respective changes of the corneal curvature.

  10. Development and validation of a wear model for the analysis of the wheel profile evolution in railway vehicles

    Science.gov (United States)

    Auciello, J.; Ignesti, M.; Malvezzi, M.; Meli, E.; Rindi, A.

    2012-11-01

    the wheel profile, obtaining the correspondent worn wheel geometry. The wheel wear evolution is reproduced by dividing the overall chosen mileage to be simulated in discrete spatial steps: at each step, the dynamic simulations are performed by means of the 3D multibody model keeping the wheel profile constant, while the wheel geometry is updated through the wear model only at the end of the discrete step. Thus, the two parts of the whole model work alternately until the completion of the whole established mileage. Clearly, the choice of an appropriate step length is one of the most important aspects of the procedure and it directly affects the result accuracy and the required computational time to complete the analysis. The whole model has been validated using experimental data relative to tests performed with the ALn 501 'Minuetto' vehicle in service on the Aosta-Pre Saint Didier track; this work has been carried out thanks to a collaboration with Trenitalia S.p.A and Rete Ferroviaria Italiana, which have provided the necessary technical data and experimental results.

  11. Analysis of Chemokines and Receptors Expression Profile in the Myelin Mutant Taiep Rat

    Directory of Open Access Journals (Sweden)

    Guadalupe Soto-Rodriguez

    2015-01-01

    Full Text Available Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT2 Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4, which might account for the demyelination in the taiep rat.

  12. Urological injuries during obstetric and gynaecological procedures: a retrospective analysis over a period of eleven years

    OpenAIRE

    Rashmi S. Desai; Sunil Kumar K.

    2016-01-01

    Background: To determine frequency and nature of urological injuries in obstetric and gynaecological procedures and their consequences and mode of management in a tertiary care hospital SDM Medical college and hospital, Dharwad, India. Methods: A retrospective study of all obstetric and gynaecological surgeries over a period of 11 years from January 2004 to December 2014 was carried out at Department of Obstetrics and Gynaecology, SDM Medical College and Hospital, Dharwad, India. Cases wit...

  13. Procedural fairness and the legitimacy of laws in Hungary: An empirical analysis

    OpenAIRE

    Boda Zsolt; Medve-Bálint Gergő

    2015-01-01

    The 2010 election in Hungary resulted in the victory of Fidesz, a conservative party which acquired a comfortable majority in the parliament and used it to initiate a number of important policy reforms. However, there are good reasons to think that both the way these reforms were adopted and their content violated a number of procedural fairness norms, such as participation in decision making and even some aspects of the rule of law. According to David Beet...

  14. Development of a unified numerical procedure for free vibration analysis of structures

    Science.gov (United States)

    Gupta, K. K.

    1981-01-01

    This paper presents the details of a unified numerical algorithm and the associated computer program developed for the efficient determination of natural frequencies and modes of free vibration of structures. Both spinning and nonspinning structures with or without viscous and/or structural damping may be solved by the current procedure; in addition, the program is capable of solving static problems with multiple load cases as well as the quadratic matrix eigenproblem associated with a finite dynamic element structural discretization.

  15. A semi-automated image analysis procedure for in situ plankton imaging systems.

    Science.gov (United States)

    Bi, Hongsheng; Guo, Zhenhua; Benfield, Mark C; Fan, Chunlei; Ford, Michael; Shahrestani, Suzan; Sieracki, Jeffery M

    2015-01-01

    Plankton imaging systems are capable of providing fine-scale observations that enhance our understanding of key physical and biological processes. However, processing the large volumes of data collected by imaging systems remains a major obstacle for their employment, and existing approaches are designed either for images acquired under laboratory controlled conditions or within clear waters. In the present study, we developed a semi-automated approach to analyze plankton taxa from images acquired by the ZOOplankton VISualization (ZOOVIS) system within turbid estuarine waters, in Chesapeake Bay. When compared to images under laboratory controlled conditions or clear waters, images from highly turbid waters are often of relatively low quality and more variable, due to the large amount of objects and nonlinear illumination within each image. We first customized a segmentation procedure to locate objects within each image and extracted them for classification. A maximally stable extremal regions algorithm was applied to segment large gelatinous zooplankton and an adaptive threshold approach was developed to segment small organisms, such as copepods. Unlike the existing approaches for images acquired from laboratory, controlled conditions or clear waters, the target objects are often the majority class, and the classification can be treated as a multi-class classification problem. We customized a two-level hierarchical classification procedure using support vector machines to classify the target objects ( 95%). First, histograms of oriented gradients feature descriptors were constructed for the segmented objects. In the first step all non-target and target objects were classified into different groups: arrow-like, copepod-like, and gelatinous zooplankton. Each object was passed to a group-specific classifier to remove most non-target objects. After the object was classified, an expert or non-expert then manually removed the non-target objects that could not be removed

  16. The potential of lacquer-peel soil profiles for palaeo-geochemical analysis using XRF analysis

    NARCIS (Netherlands)

    Arnoldussen, Stijn; van Os, B.J.H.

    2015-01-01

    This paper discusses the suitability of hand-held XRF analysis to extract palaeo-geochemical information from lacquer-peel soil sections that have been taken to document pedological information at geological and archaeological sites. This not only allows the study of sections from archaeological and

  17. Bootstrap-based procedures for inference in nonparametric receiver-operating characteristic curve regression analysis.

    Science.gov (United States)

    Rodríguez-Álvarez, María Xosé; Roca-Pardiñas, Javier; Cadarso-Suárez, Carmen; Tahoces, Pablo G

    2017-01-01

    Prior to using a diagnostic test in a routine clinical setting, the rigorous evaluation of its diagnostic accuracy is essential. The receiver-operating characteristic curve is the measure of accuracy most widely used for continuous diagnostic tests. However, the possible impact of extra information about the patient (or even the environment) on diagnostic accuracy also needs to be assessed. In this paper, we focus on an estimator for the covariate-specific receiver-operating characteristic curve based on direct regression modelling and nonparametric smoothing techniques. This approach defines the class of generalised additive models for the receiver-operating characteristic curve. The main aim of the paper is to offer new inferential procedures for testing the effect of covariates on the conditional receiver-operating characteristic curve within the above-mentioned class. Specifically, two different bootstrap-based tests are suggested to check (a) the possible effect of continuous covariates on the receiver-operating characteristic curve and (b) the presence of factor-by-curve interaction terms. The validity of the proposed bootstrap-based procedures is supported by simulations. To facilitate the application of these new procedures in practice, an R-package, known as npROCRegression, is provided and briefly described. Finally, data derived from a computer-aided diagnostic system for the automatic detection of tumour masses in breast cancer is analysed.

  18. Observer accuracy and behavior analysis: Data collection procedures on hand hygiene compliance in a neurovascular unit.

    Science.gov (United States)

    Hinz, Krista L; McGee, Heather M; Huitema, Bradley E; Dickinson, Alyce M; Van Enk, Richard A

    2014-10-01

    Although observational studies are popular, little has been done to study the integrity of human observers and the data collection process. Issues of data collection integrity threaten functional findings, leading to problematic interpretation and decreased replication. In our study the response effort associated with hand hygiene data collection in a hospital setting was manipulated using an altered data collection tool. A counterbalanced ABAB design was implemented across 2 semesters of a hand hygiene data collection practicum course. When response effort increased, compliant audits decreased and when response effort decreased, compliant audits increased. These results were statistically significant, with an overall level change z that had a P value of .001 (first semester) and .007 (second semester). These findings may warrant an increased awareness of data collection procedures where recording options include a less effortful response. The results of our study support basic research on response effort and choice behavior in an applied setting, bringing into question the integrity of data collection procedures and the integrity of the data collected. These results also suggest the need for standardizing reporting systems to ensure hand hygiene collection and reporting procedures are comparable across settings. Copyright © 2014 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

  19. Profile analysis of after-effects experienced during exposure to several virtual reality environments

    Science.gov (United States)

    Kennedy, Robert S.; Jones, Marshall B.; Lilienthal, Michael G.; Harm, Deborah L.

    1994-01-01

    Motion sickness symptoms are an unwanted by-product of exposure to virtual environments. This problem is not new and was reported in the early flight simulators and experiments on ego motions and vection. The cardinal symptom of motion sickness is, of course, vomiting, but this symptom is ordinarily preceded by a variety of other symptoms. In his classic studies of motion sickness conducted before and during World War II, G. R. Wendt introduced a three point scale to score motion sickness beyond a vomit/no vomit dichotomy. Later, Navy scientists developed a Motion Sickness Questionnaire (MSQ), originally for use in a slowly rotating room. In the last 20 years the MSQ has been used in a series of studies of air, sea, and space sickness. Only recently, however, has it been appreciated that symptom patterns in the MSQ are not uniform but vary with the way sickness is induced. In seasickness, for example, nausea is the most prominent symptom. In Navy simulators, however, the most common symptom is eye strain, especially when cathode ray tubes are employed in the simulation. The latter result was obtained in a survey of over 1,500 pilot exposures. Using this database, Essex scientists conducted a factor analysis of the MSQ. We found that signs and symptoms of motion sickness fell mainly into three clusters: 1) oculomotor disturbance, 2) nausea and related neurovegetative problems, and 3) disorientation, ataxia, and vertigo. We have since rescored the MSQ results obtained in Navy simulators in terms of these three components. We have also compared these and other profiles obtained from three different vitual reality systems to profiles obtained in sea sickness, space sickness, and alcohol intoxication. We will show examples of these various profiles and point out simularities and differences among them which indicate aspects of what might be called 'virtual-reality sickness'.

  20. An integrated portfolio optimisation procedure based on data envelopment analysis, artificial bee colony algorithm and genetic programming

    Science.gov (United States)

    Hsu, Chih-Ming

    2014-12-01

    Portfolio optimisation is an important issue in the field of investment/financial decision-making and has received considerable attention from both researchers and practitioners. However, besides portfolio optimisation, a complete investment procedure should also include the selection of profitable investment targets and determine the optimal timing for buying/selling the investment targets. In this study, an integrated procedure using data envelopment analysis (DEA), artificial bee colony (ABC) and genetic programming (GP) is proposed to resolve a portfolio optimisation problem. The proposed procedure is evaluated through a case study on investing in stocks in the semiconductor sub-section of the Taiwan stock market for 4 years. The potential average 6-month return on investment of 9.31% from 1 November 2007 to 31 October 2011 indicates that the proposed procedure can be considered a feasible and effective tool for making outstanding investment plans, and thus making profits in the Taiwan stock market. Moreover, it is a strategy that can help investors to make profits even when the overall stock market suffers a loss.

  1. An automatic flow injection analysis procedure for photometric determination of ethanol in red wine without using a chromogenic reagent.

    Science.gov (United States)

    Borges, Sivanildo S; Frizzarin, Rejane M; Reis, Boaventura F

    2006-05-01

    An automatic reagentless photometric procedure for the determination of ethanol in red wine is described. The procedure was based on a falling drop system that was implemented by employing a flow injection analysis manifold. The detection system comprised an infrared LED and a phototransistor. The experimental arrangement was designed to ensure that the wine drop grew between these devices, thus causing a decrease in the intensity of the radiation beam coming from the LED. Since ethanol content affected the size of the wine drop this feature was exploited to develop an analytical procedure for the photometric determination of ethanol in red wine without using a chromogenic reagent. In an attempt to prove the usefulness of the proposed procedure, a set of red wines were analysed. No significant difference between our results and those obtained with a reference method was observed at the 95% confidence level. Other advantages of our method were a linear response ranging from 0.17 up to 5.14 mol L(-1) (1.0 up to 30.0%) ethanol (R = 0.999); a limit of detection of 0.05 mol L(-1) (0.3%) ethanol; a relative standard deviation of 2.5% (n = 10) using typical wine sample containing 2.14 mol L(-1) (12.5%) ethanol; and a sampling rate of 50 determinations per hour.

  2. Signal detection activity on EudraVigilance data: analysis of the procedure and findings from an Italian Regional Centre for Pharmacovigilance.

    Science.gov (United States)

    Monaco, Luca; Melis, Mauro; Biagi, Chiara; Donati, Monia; Sapigni, Ester; Vaccheri, Alberto; Motola, Domenico

    2017-03-01

    In July 2012 a new European legislation (Directive 2010/84/EU and Regulation No. 1235/2010) regarding pharmacovigilance has become effective. It has boosted the activity of Signal Detection through a monthly analysis of potential safety signals on EudraVigilance (EV). Our aim is to describe the procedure of signal detection on EV data and to present results obtained by the our pharmacovigilance centre. Data are extracted from EV database, which collects suspected Adverse Drug Reactions (ADRs) of medicinal products in Europe. We are appointed to supervise digoxin, nicardipine, delapril, manidipine and hydrochlorothiazide/ramipril. ADRs are coded through MedDRA Preferred Terms and collected in the electronic Reaction Monitoring Report (eRMR). Statistical analysis is based on the Proportional Reporting Ratio (PRR) as a measure of disproportionality. Up to April 2016 we have analyzed 45 eRMR for each drug. Two signals for nicardipine were submitted to the Pharmacovigilance Risk Assessment Committee of European Medicines Agency (EMA): acute pulmonary oedema (off-label use as tocolytic) and thrombocytopenia. Our experience shows the scientific and regulatory value of signal detection activity on EV data in order to continuously evaluate the benefit/risk profile of recent and older drugs.

  3. A meta-analysis of antimicrobial susceptibility profiles for pneumococcal pneumonia in sub-Saharan Africa.

    Science.gov (United States)

    Iroh Tam, Pui-Ying; Sadoh, Ayebo E; Obaro, Stephen K

    2018-02-01

    Pneumonia causes an enormous burden of childhood disease globally, particularly in low- and middle-income countries. Pneumococcus is the most common bacterial aetiology of pneumonia; however, antimicrobials are limited and may not adequately address the local epidemiology of the region. To undertake a review and meta-analysis of pneumonia studies in sub-Saharan Africa to evaluate antimicrobial susceptibility patterns in childhood pneumonia. Articles published in PubMed and Google between 2006 and 2016 which evaluated antimicrobial susceptibility profiles of pneumococcal pneumonia in children in sub-Saharan Africa were identified. The source of specimens, pathogens and antimicrobial susceptibility data were extracted. Pooled analysis of susceptible isolates was conducted using random effects models. Children from 15 studies and 1634 isolates were included in the meta-analysis. In cases of childhood pneumonia, the mean overall proportion of penicillin susceptibility from invasive specimens of Streptococcus pneumoniae was 85.7% (95% CI 80.1-91.3), and of trimethoprim-sulfamethoxazole was 21.0% (95% CI 5.1-36.9). Compared with all S. pneumoniae specimens, penicillin susceptibility was 68.6% (95% CI 59.6-77.5) and that of trimethoprim-sulfamethoxazole was 26.3% (95% CI 14.1-38.6). A high level of heterogeneity was detected, reflecting the paucity of data available. The establishment of national and regional diagnostic platforms to monitor antimicrobial susceptibility profiles for pneumonia as well as other invasive diseases will provide data with which to assess the relevance and adaptation of antimicrobial prescribing recommendations.

  4. Banking placental tissue: An optimized collection procedure for genome-wide analysis of nucleic acids

    Science.gov (United States)

    Wolfe, L.M.; Thiagarajan, R.D.; Boscolo, F.; Taché, V.; Coleman, R.L.; Kim, J.; Kwan, W.K.; Loring, J.F.; Parast, M.; Laurent, L.C.

    2016-01-01

    Introduction Banking of high-quality placental tissue specimens will enable biomarker discovery and molecular studies on diseases involving placental dysfunction. Systematic studies aimed at developing feasible standardized methodology for placental collection in a typical clinical setting are lacking. Methods To determine the acceptable timeframe for placental collection, we collected multiple samples from first and third trimester placentas at serial timepoints in a 2-h window after delivery, simultaneously comparing the traditional snap-freeze technique to commercial solutions designed to preserve RNA (RNAlater™), and DNA (DNAgard®). The performance of RNAlater for preserving DNA was also tested. Nucleic acid quality was assessed by determining the RNA integrity number (RIN) and genome-wide microarray profiling for gene expression and DNA methylation. Results We found that samples collected in RNAlater had higher and more consistent RINs compared to snap-frozen tissue. Similar RINs were obtained for tissue collected in RNAlater as large (1 cm3) and small (∼0.1 cm3) pieces. RNAlater appeared to better stabilize the time zero gene expression profile compared to snap-freezing for first trimester placenta. DNA methylation profiles remained quite stable over a 2 h time period after removal of the placenta from the uterus, with DNAgard being superior to other treatments. Discussion and conclusion The collection of placental samples in RNAlater and DNAgard is simple, and eliminates the need for liquid nitrogen or a freezer on-site. Moreover, the quality of the nucleic acids and the resulting data from samples collected in these preservation solutions is higher than samples collected using the snap-freeze method and easier to implement in busy clinical environments. PMID:24951174

  5. Investigation of medium-term barred beach behavior using 28-year beach profile data and Rotated Empirical Orthogonal Function analysis

    Science.gov (United States)

    Kuriyama, Yoshiaki; Yanagishima, Shinichi

    2016-05-01

    A 28-year beach profile dataset for a stretch of the Hasaki coast in Japan was examined using Rotated Empirical Orthogonal Function (REOF) analysis to investigate the cross-shore variation in the characteristics of beach profile change. The data were obtained weekly, on a micro-tidal wave-dominated intermediate beach, along a survey line extending from the backshore to a water depth of approximately 5 m. REOF analysis using the first eight empirical orthogonal functions led to the study area being divided into five unique zones based on beach profile change patterns, namely the backshore, the foreshore, the inner and outer transition zones and the bar-trough zone. Although these zones were notably distinct from one another, the profiles in foreshore and the shoreward part of the inner transition zone changed in the same way over periods of 6 and 12 months.

  6. A Comparative Study of the Analysis, Numerical Modelling and Experimental Test on a Sandwich Panel with Plane and Profiled Facings

    Directory of Open Access Journals (Sweden)

    Raluca Hohan

    2010-01-01

    Full Text Available Sandwich panels are remarkable products because they can be as strong as a solid material but with less weight. The analysis that is required to predict the stresses and deflections in panels with flat or lightly profiled facings is that of conventional beam theory but with the addition of shear deformation. Knowing that the profiled sheets bring an increase of the flexural stiffness, formulas showing the calculus of a panel with flat and profiled facings are established. A comparison between the results of a mathematical calculus, an experimental test and a numerical modelling is provided.

  7. MethVisual - visualization and exploratory statistical analysis of DNA methylation profiles from bisulfite sequencing.

    Science.gov (United States)

    Zackay, Arie; Steinhoff, Christine

    2010-12-15

    Exploration of DNA methylation and its impact on various regulatory mechanisms has become a very active field of research. Simultaneously there is an arising need for tools to process and analyse the data together with statistical investigation and visualisation. MethVisual is a new application that enables exploratory analysis and intuitive visualization of DNA methylation data as is typically generated by bisulfite sequencing. The package allows the import of DNA methylation sequences, aligns them and performs quality control comparison. It comprises basic analysis steps as lollipop visualization, co-occurrence display of methylation of neighbouring and distant CpG sites, summary statistics on methylation status, clustering and correspondence analysis. The package has been developed for methylation data but can be also used for other data types for which binary coding can be inferred. The application of the package, as well as a comparison to existing DNA methylation analysis tools and its workflow based on two datasets is presented in this paper. The R package MethVisual offers various analysis procedures for data that can be binarized, in particular for bisulfite sequenced methylation data. R/Bioconductor has become one of the most important environments for statistical analysis of various types of biological and medical data. Therefore, any data analysis within R that allows the integration of various data types as provided from different technological platforms is convenient. It is the first and so far the only specific package for DNA methylation analysis, in particular for bisulfite sequenced data available in R/Bioconductor enviroment. The package is available for free at http://methvisual.molgen.mpg.de/ and from the Bioconductor Consortium http://www.bioconductor.org.

  8. MethVisual - visualization and exploratory statistical analysis of DNA methylation profiles from bisulfite sequencing

    Directory of Open Access Journals (Sweden)

    Zackay Arie

    2010-12-01

    Full Text Available Abstract Background Exploration of DNA methylation and its impact on various regulatory mechanisms has become a very active field of research. Simultaneously there is an arising need for tools to process and analyse the data together with statistical investigation and visualisation. Findings MethVisual is a new application that enables exploratory analysis and intuitive visualization of DNA methylation data as is typically generated by bisulfite sequencing. The package allows the import of DNA methylation sequences, aligns them and performs quality control comparison. It comprises basic analysis steps as lollipop visualization, co-occurrence display of methylation of neighbouring and distant CpG sites, summary statistics on methylation status, clustering and correspondence analysis. The package has been developed for methylation data but can be also used for other data types for which binary coding can be inferred. The application of the package, as well as a comparison to existing DNA methylation analysis tools and its workflow based on two datasets is presented in this paper. Conclusions The R package MethVisual offers various analysis procedures for data that can be binarized, in particular for bisulfite sequenced methylation data. R/Bioconductor has become one of the most important environments for statistical analysis of various types of biological and medical data. Therefore, any data analysis within R that allows the integration of various data types as provided from different technological platforms is convenient. It is the first and so far the only specific package for DNA methylation analysis, in particular for bisulfite sequenced data available in R/Bioconductor enviroment. The package is available for free at http://methvisual.molgen.mpg.de/ and from the Bioconductor Consortium http://www.bioconductor.org.

  9. Economics definitions, methods, models, and analysis procedures for Homeland Security applications.

    Energy Technology Data Exchange (ETDEWEB)

    Ehlen, Mark Andrew; Loose, Verne William; Vargas, Vanessa N.; Smith, Braeton J.; Warren, Drake E.; Downes, Paula Sue; Eidson, Eric D.; Mackey, Greg Edward

    2010-01-01

    This report gives an overview of the types of economic methodologies and models used by Sandia economists in their consequence analysis work for the National Infrastructure Simulation & Analysis Center and other DHS programs. It describes the three primary resolutions at which analysis is conducted (microeconomic, mesoeconomic, and macroeconomic), the tools used at these three levels (from data analysis to internally developed and publicly available tools), and how they are used individually and in concert with each other and other infrastructure tools.

  10. The Geomorphic Road Analysis and Inventory Package (GRAIP) Volume 2: Office Procedures

    Science.gov (United States)

    Richard M. Cissel; Thomas A. Black; Kimberly A. T. Schreuders; Ajay Prasad; Charles H. Luce; David G. Tarboton; Nathan A. Nelson

    2012-01-01

    An important first step in managing forest roads for improved water quality and aquatic habitat is the performance of an inventory. The Geomorphic Roads Analysis and Inventory Package (GRAIP) was developed as a tool for making a comprehensive inventory and analysis of the effects of forest roads on watersheds. This manual describes the data analysis and process of a...

  11. Comments on Functional Analysis Procedures for School-Based Behavior Problems.

    Science.gov (United States)

    Repp, Alan

    1994-01-01

    This article reviews the development of functional analysis in understanding behavior problems like self-injury and aggression and, more recently, school-based problems. Seen as particularly promising is current work on the analysis of setting events and analysis of dependent variables by considering the effects of intervention on response…

  12. Socioeconomic Profile of Consumers of Restaurants as Regards Organic Products: A Comparative Analysis

    Directory of Open Access Journals (Sweden)

    Belquis Oliveira Meireles

    2016-05-01

    Full Text Available This article aims to analyze comparatively the socioeconomic profile of consumers who value and those who do not value organic products in restaurants. To achieve this purpose, 392 customers were interviewed in two restaurants from a large supermarket network by means of a structured questionnaire. Regarding the cross-analysis between the profile of consumers and the enhancement of organic products, the research has found out that: (a women value most organic products than men; (b the higher the educational level of analyzed individuals, the greater the appreciation shown by them on the subject; (c consumers with an income above three minimum wages value more organic products than persons with a lower income; however, from three minimum wages, the percentage of the valuation remains; and (d consumers over 40 years of age value more organic products than younger consumers. It is emphasized that the findings presented in this article contribute to the formation of the body of scientific knowledge about organic consumers, that does not exclude, however, their need to be considered with caution, considering that they do not allow inferences that go beyond the investigated sample. As an empirical contribution, so, the broader understanding of these consumers, so as to better serve them, becomes an important aspect so shops can succeed.

  13. Integrated analysis of drug-induced gene expression profiles predicts novel hERG inhibitors.

    Directory of Open Access Journals (Sweden)

    Joseph J Babcock

    Full Text Available Growing evidence suggests that drugs interact with diverse molecular targets mediating both therapeutic and toxic effects. Prediction of these complex interactions from chemical structures alone remains challenging, as compounds with different structures may possess similar toxicity profiles. In contrast, predictions based on systems-level measurements of drug effect may reveal pharmacologic similarities not evident from structure or known therapeutic indications. Here we utilized drug-induced transcriptional responses in the Connectivity Map (CMap to discover such similarities among diverse antagonists of the human ether-à-go-go related (hERG potassium channel, a common target of promiscuous inhibition by small molecules. Analysis of transcriptional profiles generated in three independent cell lines revealed clusters enriched for hERG inhibitors annotated using a database of experimental measurements (hERGcentral and clinical indications. As a validation, we experimentally identified novel hERG inhibitors among the unannotated drugs in these enriched clusters, suggesting transcriptional responses may serve as predictive surrogates of cardiotoxicity complementing existing functional assays.

  14. Analysis of plasmid genes by phylogenetic profiling and visualization of homology relationships using Blast2Network

    Directory of Open Access Journals (Sweden)

    Bazzicalupo Marco

    2008-12-01

    Full Text Available Abstract Background Phylogenetic methods are well-established bioinformatic tools for sequence analysis, allowing to describe the non-independencies of sequences because of their common ancestor. However, the evolutionary profiles of bacterial genes are often complicated by hidden paralogy and extensive and/or (multiple horizontal gene transfer (HGT events which make bifurcating trees often inappropriate. In this context, plasmid sequences are paradigms of network-like relationships characterizing the evolution of prokaryotes. Actually, they can be transferred among different organisms allowing the dissemination of novel functions, thus playing a pivotal role in prokaryotic evolution. However, the study of their evolutionary dynamics is complicated by the absence of universally shared genes, a prerequisite for phylogenetic analyses. Results To overcome such limitations we developed a bioinformatic package, named Blast2Network (B2N, allowing the automatic phylogenetic profiling and the visualization of homology relationships in a large number of plasmid sequences. The software was applied to the study of 47 completely sequenced plasmids coming from Escherichia, Salmonella and Shigella spps. Conclusion The tools implemented by B2N allow to describe and visualize in a new way some of the evolutionary features of plasmid molecules of Enterobacteriaceae; in particular it helped to shed some light on the complex history of Escherichia, Salmonella and Shigella plasmids and to focus on possible roles of unannotated proteins. The proposed methodology is general enough to be used for comparative genomic analyses of bacteria.

  15. Analysis of the vocal profiles of male seduction: from exhibition to self-disclosure.

    Science.gov (United States)

    Anolli, Luigi; Ciceri, Rita

    2002-04-01

    The authors analyzed the suprasegmental (temporary) vocal profiles of 19 young men (not married) during their seductive interaction with 19 young women (not married) whom they had not known previously. The aim of the research was to verify the specificity, the sequential temporal stream, and the efficacy of the acoustical features of the seducer's voice during seductive interaction. In an ecological laboratory situation, the male participants were asked to meet their female partners. Participant-by-participant analysis showed that the male seductive voice is characterized by strong variations during the course of the seductive sequence. At the beginning of the sequence it had a higher pitch and an elevated intensity, as well as a faster rate of articulation than it did during normal speech (the exhibition voice). In the following phases it became gradually lower, weaker, and warmer (the self-disclosure voice). The modulation and variability of the vocal profiles during the seductive interaction were significantly stressed in the 9 successful seducers (those who succeeded in arranging a subsequent meeting with the partner), compared with the 10 unsuccessful seducers. The results have been interpreted as an ability to perform an effective local management of seductive communication.

  16. Comparative profiling analysis of woody flavouring from vine-shoots and oak chips.

    Science.gov (United States)

    Delgado de la Torre, M Pilar; Priego-Capote, Feliciano; Luque de Castro, M Dolores

    2014-02-01

    Woody liquid flavourings extracted from different varieties of vine shoots and oak chips have been characterized by gas chromatography-mass spectrometry to compare the profile of compounds as potential contributors to the organoleptical properties of wine and spirits aged in oak barrels. Oak chips are frequently added to barrels to accelerate the ageing process, while vine shoots are produced in high amounts in wine-producing countries. The extracts were isolated by superheated liquid extraction (SHLE) after optimization of extraction variables. The SHLE protocol was performed using ethanol-water mixtures (pH 3) at 220 °C for 60 min. Compounds were identified using NIST databases, and the resulting profile was used as a dataset for qualitative and semi-quantitative comparison between extracts obtained from different varieties of vine shoots and oak chips. Statistical analysis enabled demonstration of the similarity among extracts from vine shoots and oak wood, providing the first study on this subject. The special role of phenols and furanic derivatives has been described. This study is the first stage for characterization of vine shoots as a by-product with potential for use in the oenological field. © 2013 Society of Chemical Industry.

  17. Slogger: A Profiling and Analysis System Based on Semantic Web Technologies

    Directory of Open Access Journals (Sweden)

    Mark Baker

    2008-01-01

    Full Text Available Increasingly, distributed systems are being used to host all manner of applications. While these platforms provide a relatively cheap and effective means of executing applications, so far there has been little work in developing tools and utilities that can help application developers understand problems with the supporting software, or the executing applications. To fully understand why an application executing on a distributed system is not behaving as would be expected it is important that not only the application, but also the underlying middleware, and the operating system are analysed too, otherwise issues could be missed and certainly overall performance profiling and fault diagnoses would be harder to understand. We believe that one approach to profiling and the analysis of distributed systems and the associated applications is via the plethora of log files generated at runtime. In this paper we report on a system (Slogger, that utilises various emerging Semantic Web technologies to gather the heterogeneous log files generated by the various layers in a distributed system and unify them in common data store. Once unified, the log data can be queried and visualised in order to highlight potential problems or issues that may be occurring in the supporting software or the application itself.

  18. Micropropagation and protein profile analysis by SDS-PAGE of Gracilaria changii (Rhodophyta, Solieriaceae

    Directory of Open Access Journals (Sweden)

    Lin Wei Jong

    2015-05-01

    Full Text Available Gracilaria changii seaweed is primarily important as a source of agar with wide applications in food industries. The high demand of agar led to gradual depletion of G. changii in natural resources. Establishment of in vitro culture of G. changii has an important role and allowing G. changii explants to grow optimally under controlled conditions to provide constant, continuous and sufficient seedlings supply for Gracilaria farming. This study focused on micropropagation culture of G. changii in which different exogenous factors influencing seaweed growth were investigated: strength of chosen medium Provasoli’s enriched seawater (PES, types and concentration of fertilizers/biostimulant, supplementation of plant growth regulators and seawater salinity. The results were presented in daily growth rate of explants and data analysis was carried out using one-way ANOVA. The results demonstrated high growth rate of G. changii in 25% of PES supplemented with 5 mg L−1 AMPEP, and seawater salinity range between 30 and 40 ppt, respectively. Protein profiles of tissue-cultured and farm cultivated G. changii were produced by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE. The results demonstrated no remarkable difference in the protein profiles and indicated the suitability of the culture condition for the growth of G. changii.

  19. The interface of language proficiency and identity: a profile analysis of bilingual adolescents and their writing.

    Science.gov (United States)

    Danzak, Robin L

    2011-10-01

    The purpose of this study was to explore how adolescent English language learners' (ELLs') language and literacy experiences impacted their identities as bilingual writers. Six students were randomly selected from a group of 20 Spanish-speaking ELLs, ages 11-14, who participated in a larger, mixed-methods study on bilingual writing (see Danzak, 2011). The participants produced 10 written journal entries in their language of choice (English, Spanish, or both) and were interviewed. Qualitative analyses were applied to the participants' writing and interviews, both individually and cross-case. Findings were integrated to some extent with the outcomes of quantitative measures applied to the students' writing. Three patterns emerged: ethnic differences, language discrimination, and language preference. Also, the students' self-identification as monolingual or bilingual was reflected in their attitudes toward language learning and their outcomes on writing measures. Three portraits of emerging bilingual writers are discussed: struggling emerging, dominant emerging, and balanced emerging. Language and literacy learning strategies are recommended for each. Qualitative profiles of adolescent ELLs offer an understanding of students' experiences and identities that augments information provided by quantitative writing measures. Additionally, a mixed-methods profile analysis may aid in the identification of adolescent ELLs who may be struggling with undiagnosed language learning disabilities.

  20. Risk factor profiles among intravenous drug using young adults: a latent class analysis (LCA) approach.

    Science.gov (United States)

    James, Sigrid; McField, Edward S; Montgomery, Susanne B

    2013-03-01

    Using data from a cross-sectional study that examined health risk behaviors among urban intravenous drug-using (IDU) adolescents and young adults, this study investigated risk profiles among a high-risk sample (n=274). Risk profiles were empirically derived through latent class analysis based on indicators of engagement in health-risking behaviors, experience of abuse and violence as well as individual and family risk factors. The best fitting model was a 3-class model. Class 1 (n=95) captured participants with the lowest risk across all indicators. Compared to Class 1, Class 2 (n=128) and Class 3 (n=51) had elevated rates of engagement in health-risking behaviors as well as individual and family risk factors; however, Class 3 had the highest rate of engagement in sexual risk behavior, and backgrounds of substantial abuse and violence as well as familial psychopathology. Class 2 was the group most socioeconomically disadvantaged, with the highest percentage of participants coming from poor backgrounds, spending the longest time homeless and working the fewest months. Identifying subgroups of IDU has the potential to guide the development of more targeted and effective strategies for prevention and treatment of this high-risk population. Copyright © 2012 Elsevier Ltd. All rights reserved.