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Sample records for productive failure pf

  1. Comparing Learning from Productive Failure and Vicarious Failure

    Science.gov (United States)

    Kapur, Manu

    2014-01-01

    A total of 136 eighth-grade math students from 2 Singapore schools learned from either productive failure (PF) or vicarious failure (VF). PF students "generated" solutions to a complex problem targeting the concept of variance that they had not learned yet before receiving instruction on the targeted concept. VF students…

  2. Influence of Nitrogen Source on 2,4-diacetylphloroglucinol Production by the Biocontrol Strain Pf-5

    OpenAIRE

    M, Hultberg; B, Alsanius

    2008-01-01

    The production of 2,4-diacetylphloroglucinol (DAPG) by the biocontrol agent Pseudomonas fluorescens Pf-5 was studied in nutrient-solution based media with varying nitrogen content. No production of DAPG was observed when organic nitrogen was omitted from the media, regardless of the inorganic nitrogen source used. Furthermore, a micromolar concentration range of organic nitrogen was insufficient to sustain production. When a millimolar concentration range of organic nitrogen was used, DAPG pr...

  3. New product failure

    DEFF Research Database (Denmark)

    Dijksterhuis, Garmt Bernard

    2016-01-01

    When new consumer products are developed and later launched, 50 to 75 percent of them are removed from the market far short of meeting their projected financial targets. In short: they fail. We conclude that this failure is due to institutionalized insufficiencies in the use of the sciences...... that are best geared to understand and predict consumer behaviour, viz. the behavioural sciences. These are not necessarily the same as the marketing science that is performed by marketing departments. A scientific approach to understanding consumer behaviour appears to be lacking in many corporate research...

  4. On-line fission products measurements during a PWR severe accident: the French DECA-PF project

    Energy Technology Data Exchange (ETDEWEB)

    Ducros, G.; Allinei, P.G.; Roure, C. [CEA, DEN, F-13108 Saint-Paul-lez-Durance, (France); Rozel, C. [EDF SEPTEN, 12-14 Avenue Dutrievoz, F-69628, Villeurbanne, (France); Blanc De Lanaute, N. [CANBERRA, 1 rue des Herons, F-78182, Saint Quentin en Yvelines, (France); Musoyan, G. [AREVA, Tour AREVA, 1 place Jean Millier, F-92084 Paris La Defense Cedex, (France)

    2015-07-01

    Following the Fukushima accident, a lot of recommendations was drawn by international organizations (IAEA, OECD, NUGENIA network...) in order to improve the safety in such accidental conditions and mitigate their consequences. One of these recommendations was to improve the robustness of the instrumentation, which was dramatically lacking at Fukushima, as well as to better determine the Source Term involved in nuclear accident. The DECA-PF project (Diagnosis of a degraded reactor core through Fission Product measurements) was elaborated in this context and selected as one of 21 collaborative R and D projects in the field of nuclear safety and radioprotection, funded in May 2013 by the French National Research Agency. Over the months following the Fukushima accident, a CEA crisis team was held in order to analyze on-line the situation taking into account the data delivered by TEPCO and other organizations. Despite the difficulties encountered concerning the reliability of these data, the work performed showed the high capacity of Fission Products (FP) measurements to get a diagnosis relative to the status of the reactors and the spent fuel pools (SFP). Based on these FP measurements, it was possible to conclude that the main origin of the releases was coming from the cores and not from the SFP, in particular for SFP-4 which was of high concern, and that the degradation level of the reactors was very large, including probably an extensive core melting. To improve the reliability of this kind of diagnosis, the necessity to get such measurements as soon as possible after the accident and as near as possible from the reactor was stressed. In this way the present DECA-PF project intends to develop a new and innovative instrumentation taking into account the design of the French nuclear power plants on which sand bed filters have been implemented for severe accident management. Three complementary techniques, devoted to measure the FP release on-line, are being studied

  5. Comparison of SCDAP/RELAP5/MOD3 to TRAC-PF1/MOD1 for timing analysis of PWR fuel pin failures

    International Nuclear Information System (INIS)

    Jones, K.R.; Katsma, K.R.; Wade, N.L.; Siefken, L.J.; Straka, M.

    1991-01-01

    A comparison has been made of SCDAP/RELAP5/MOD3- and TRAC-PF1/MOD1- based calculations of the fuel pin failure timing (time from containment isolation signal to first fuel pin failure) in a loss-of-coolant accident (LOCA). The two codes were used to calculate the thermal-hydraulic boundary conditions for a complete, double-ended, offset-shear break of a cold leg in a Westinghouse 4-loop pressurized water reactor. Both calculations used the FRAPCON-2 code to calculate the steady-state fuel rod behavior and the FRAP-T6 code to calculate the transient fuel rod behavior. The analysis was performed for 16 combinations of fuel burnups and power peaking factors extending up to the Technical Specifications limits. While all calculations were made on a best-estimate basis, the SCDAP/RELAP5/MOD3 code has not yet been fully assessed for large-break LOCA analysis. The results indicate that SCDAP/RELAP5/MOD3 yields conservative fuel pin failure timing results in comparison to those generated using TRAC-PF1/MOD1. 7 refs., 5 figs

  6. Comparison of TRAC-PF1/MOD1 to a no-failure UPI test in the Cylindrical Core Test Facility

    International Nuclear Information System (INIS)

    Cappiello, M.; Spore, J.

    1986-01-01

    TRAC-PF1/MOD1 is compared to a no-failure upper plenum injection reflood test in the Cylindrical Core Test Facility. The results show that TRAC can accurately predict the asymmetric channeling of fluid from upper plenum into the core and that a multidimensional modeling capability is required to do so. The rod temperature behavior is accurately predicted for both the peak cladding temperature and the quench time in the high- and low-power zones. Excessive downflow of liquid at the tie plate is predicted as a result of the interfacial drag model used in TRAC. 10 figs

  7. No apparent costs for facultative antibiotic production by the soil bacterium Pseudomonas fluorescens Pf0-1.

    Science.gov (United States)

    Garbeva, Paolina; Tyc, Olaf; Remus-Emsermann, Mitja N P; van der Wal, Annemieke; Vos, Michiel; Silby, Mark; de Boer, Wietse

    2011-01-01

    Many soil-inhabiting bacteria are known to produce secondary metabolites that can suppress microorganisms competing for the same resources. The production of antimicrobial compounds is expected to incur fitness costs for the producing bacteria. Such costs form the basis for models on the co-existence of antibiotic-producing and non-antibiotic producing strains. However, so far studies quantifying the costs of antibiotic production by bacteria are scarce. The current study reports on possible costs, for antibiotic production by Pseudomonas fluorescens Pf0-1, a soil bacterium that is induced to produce a broad-spectrum antibiotic when it is confronted with non-related bacterial competitors or supernatants of their cultures. We measured the possible cost of antibiotic production for Pseudomonas fluorescens Pf0-1 by monitoring changes in growth rate with and without induction of antibiotic production by supernatant of a bacterial competitor, namely Pedobacter sp.. Experiments were performed in liquid as well as on semi-solid media under nutrient-limited conditions that are expected to most clearly reveal fitness costs. Our results did not reveal any significant costs for production of antibiotics by Pseudomonas fluorescens Pf0-1. Comparison of growth rates of the antibiotic-producing wild-type cells with those of non-antibiotic producing mutants did not reveal costs of antibiotic production either. Based on our findings we propose that the facultative production of antibiotics might not be selected to mitigate metabolic costs, but instead might be advantageous because it limits the risk of competitors evolving resistance, or even the risk of competitors feeding on the compounds produced.

  8. No apparent costs for facultative antibiotic production by the soil bacterium Pseudomonas fluorescens Pf0-1.

    Directory of Open Access Journals (Sweden)

    Paolina Garbeva

    Full Text Available BACKGROUND: Many soil-inhabiting bacteria are known to produce secondary metabolites that can suppress microorganisms competing for the same resources. The production of antimicrobial compounds is expected to incur fitness costs for the producing bacteria. Such costs form the basis for models on the co-existence of antibiotic-producing and non-antibiotic producing strains. However, so far studies quantifying the costs of antibiotic production by bacteria are scarce. The current study reports on possible costs, for antibiotic production by Pseudomonas fluorescens Pf0-1, a soil bacterium that is induced to produce a broad-spectrum antibiotic when it is confronted with non-related bacterial competitors or supernatants of their cultures. METHODOLOGY AND PRINCIPAL FINDINGS: We measured the possible cost of antibiotic production for Pseudomonas fluorescens Pf0-1 by monitoring changes in growth rate with and without induction of antibiotic production by supernatant of a bacterial competitor, namely Pedobacter sp.. Experiments were performed in liquid as well as on semi-solid media under nutrient-limited conditions that are expected to most clearly reveal fitness costs. Our results did not reveal any significant costs for production of antibiotics by Pseudomonas fluorescens Pf0-1. Comparison of growth rates of the antibiotic-producing wild-type cells with those of non-antibiotic producing mutants did not reveal costs of antibiotic production either. SIGNIFICANCE: Based on our findings we propose that the facultative production of antibiotics might not be selected to mitigate metabolic costs, but instead might be advantageous because it limits the risk of competitors evolving resistance, or even the risk of competitors feeding on the compounds produced.

  9. An Interspecies Signaling System Mediated by Fusaric Acid Has Parallel Effects on Antifungal Metabolite Production by Pseudomonas protegens Strain Pf-5 and Antibiosis of Fusarium spp.

    Science.gov (United States)

    Quecine, Maria Carolina; Kidarsa, Teresa A.; Goebel, Neal C.; Shaffer, Brenda T.; Henkels, Marcella D.; Zabriskie, T. Mark

    2015-01-01

    Pseudomonas protegens strain Pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. We derived mutants of Pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (DAPG), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide A, and toxoflavin. These mutants were tested for inhibition of the pathogens Fusarium verticillioides and Fusarium oxysporum f. sp. pisi. Rhizoxin, pyrrolnitrin, and DAPG were found to be primarily responsible for fungal antagonism by Pf-5. Previously, other workers showed that the mycotoxin fusaric acid, which is produced by many Fusarium species, including F. verticillioides, inhibited the production of DAPG by Pseudomonas spp. In this study, amendment of culture media with fusaric acid decreased DAPG production, increased pyoluteorin production, and had no consistent influence on pyrrolnitrin or orfamide A production by Pf-5. Fusaric acid also altered the transcription of biosynthetic genes, indicating that the mycotoxin influenced antibiotic production by Pf-5 at the transcriptional level. Addition of fusaric acid to the culture medium reduced antibiosis of F. verticillioides by Pf-5 and derivative strains that produce DAPG but had no effect on antibiosis by Pf-5 derivatives that suppressed F. verticillioides due to pyrrolnitrin or rhizoxin production. Our results demonstrated the importance of three compounds, rhizoxin, pyrrolnitrin, and DAPG, in suppression of Fusarium spp. by Pf-5 and confirmed that an interspecies signaling system mediated by fusaric acid had parallel effects on antifungal metabolite production and antibiosis by the bacterial biological control organism. PMID:26655755

  10. An Interspecies Signaling System Mediated by Fusaric Acid Has Parallel Effects on Antifungal Metabolite Production by Pseudomonas protegens Strain Pf-5 and Antibiosis of Fusarium spp.

    Science.gov (United States)

    Quecine, Maria Carolina; Kidarsa, Teresa A; Goebel, Neal C; Shaffer, Brenda T; Henkels, Marcella D; Zabriskie, T Mark; Loper, Joyce E

    2015-12-11

    Pseudomonas protegens strain Pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. We derived mutants of Pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (DAPG), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide A, and toxoflavin. These mutants were tested for inhibition of the pathogens Fusarium verticillioides and Fusarium oxysporum f. sp. pisi. Rhizoxin, pyrrolnitrin, and DAPG were found to be primarily responsible for fungal antagonism by Pf-5. Previously, other workers showed that the mycotoxin fusaric acid, which is produced by many Fusarium species, including F. verticillioides, inhibited the production of DAPG by Pseudomonas spp. In this study, amendment of culture media with fusaric acid decreased DAPG production, increased pyoluteorin production, and had no consistent influence on pyrrolnitrin or orfamide A production by Pf-5. Fusaric acid also altered the transcription of biosynthetic genes, indicating that the mycotoxin influenced antibiotic production by Pf-5 at the transcriptional level. Addition of fusaric acid to the culture medium reduced antibiosis of F. verticillioides by Pf-5 and derivative strains that produce DAPG but had no effect on antibiosis by Pf-5 derivatives that suppressed F. verticillioides due to pyrrolnitrin or rhizoxin production. Our results demonstrated the importance of three compounds, rhizoxin, pyrrolnitrin, and DAPG, in suppression of Fusarium spp. by Pf-5 and confirmed that an interspecies signaling system mediated by fusaric acid had parallel effects on antifungal metabolite production and antibiosis by the bacterial biological control organism. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  11. User perception of product failures

    NARCIS (Netherlands)

    Keijzers, J.; Luyk - de Visser, I.M.; Mathijssen, R.W.M.

    2009-01-01

    Currently, the field of consumer electronics is one of the most challenging environments with respect to product design. Due to the combination of the continuous influx of new technology and the economic effects of globalization, it is now possible to create products with a functionality that was

  12. Productive failure in learning math.

    Science.gov (United States)

    Kapur, Manu

    2014-06-01

    When learning a new math concept, should learners be first taught the concept and its associated procedures and then solve problems, or solve problems first even if it leads to failure and then be taught the concept and the procedures? Two randomized-controlled studies found that both methods lead to high levels of procedural knowledge. However, students who engaged in problem solving before being taught demonstrated significantly greater conceptual understanding and ability to transfer to novel problems than those who were taught first. The second study further showed that when given an opportunity to learn from the failed problem-solving attempts of their peers, students outperformed those who were taught first, but not those who engaged in problem solving first. Process findings showed that the number of student-generated solutions significantly predicted learning outcomes. These results challenge the conventional practice of direct instruction to teach new math concepts and procedures, and propose the possibility of learning from one's own failed problem-solving attempts or those of others before receiving instruction as alternatives for better math learning. © 2014 Cognitive Science Society, Inc.

  13. Rhizoxin analogs, orfamide A and chitinase production contribute to the toxicity of Pseudomonas protegens strain Pf-5 to Drosophila melanogaster

    Science.gov (United States)

    Pseudomonas protegens strain Pf-5 is a soil bacterium that was first described for its activity in biological control of plant diseases and has since been shown to be lethal to certain insects. Among these is the fruit fly Drosophila melanogaster, a well-established model organism for studies evalu...

  14. Examining Productive Failure, Productive Success, Unproductive Failure, and Unproductive Success in Learning

    Science.gov (United States)

    Kapur, Manu

    2016-01-01

    Learning and performance are not always commensurable. Conditions that maximize performance in the initial learning may not maximize learning in the longer term. I exploit this incommensurability to theoretically and empirically interrogate four possibilities for design: productive success, productive failure, unproductive success, and…

  15. An inter-species signaling system mediated by fusaric acid has parallel effects on antifungal metabolite production by Pseudomonas protegens Pf-5 and antibiosis of Fusarium spp.

    Science.gov (United States)

    Pseudomonas protegens strain Pf-5 is a rhizosphere bacterium that acts as a biocontrol agent of soilborne plant diseases, and produces at least seven different secondary metabolites with antifungal properties. We derived site-directed mutants of Pf-5 with single and multiple mutations in the biosynt...

  16. Predictive Manufacturing: A Classification Strategy to Predict Product Failures

    DEFF Research Database (Denmark)

    Khan, Abdul Rauf; Schiøler, Henrik; Kulahci, Murat

    2018-01-01

    manufacturing analytics model that employs a big data approach to predicting product failures; third, we illustrate the issue of high dimensionality, along with statistically redundant information; and, finally, our proposed method will be compared against the well-known classification methods (SVM, K......-nearest neighbor, artificial neural networks). The results from real data show that our predictive manufacturing analytics approach, using genetic algorithms and Voronoi tessellations, is capable of predicting product failure with reasonable accuracy. The potential application of this method contributes...... to accurately predicting product failures, which would enable manufacturers to reduce production costs without compromising product quality....

  17. No apparent costs for facultative antibiotic production by the soil bacterium Pseudomonas fluorescens Pf0-1

    NARCIS (Netherlands)

    Garbeva, P.V.; Tyc, O.; Remus-Emsermann, M.N.P.; Van der Wal, A.; Vos, M.; Silby, M.W.; De Boer, W.

    2011-01-01

    Background: Many soil-inhabiting bacteria are known to produce secondary metabolites that can suppress microorganisms competing for the same resources. The production of antimicrobial compounds is expected to incur fitness costs for the producing bacteria. Such costs form the basis for models on the

  18. Fuel models and results from the TRAC-PF1/MIMAS TMI-2 accident calculation

    International Nuclear Information System (INIS)

    Schwegler, E.C.; Maudlin, P.J.

    1983-01-01

    A brief description of several fuel models used in the TRAC-PF1/MIMAS analysis of the TMI-2 accident is presented, and some of the significant fuel-rod behavior results from this analysis are given. Peak fuel-rod temperatures, oxidation heat production, and embrittlement and failure behavior calculated for the TMI-2 accident are discussed. Other aspects of fuel behavior, such as cladding ballooning and fuel-cladding eutectic formation, were found not to significantly affect the accident progression

  19. Properties of the malarial proteins Pf2, Pf9 and PfP0, which support ...

    Indian Academy of Sciences (India)

    Properties of the malarial proteins Pf2, Pf9 and PfP0, which support their roles as immune targets. Antibodies raised to each of these proteins (or purified from immune adults) inhibit the growth of Plasmodium falciparum at the red cell invasion step. The proteins are localized on the parasite cell surface. Each protein is ...

  20. Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste.

    Science.gov (United States)

    Bhange, Khushboo; Chaturvedi, Venkatesh; Bhatt, Renu

    2016-06-01

    The present study is an attempt to optimize simultaneous production of keratinolytic protease, amylase and biosurfactant from feather meal, potato peel and rape seed cake in a single media by response surface methodology to evaluate their biochemical properties for detergent additive. The optimization was carried out using 20 run, 3 factor and 5-level of central composite design on design expert software which resulted in a 1.2, 0.84 and 2.28 fold increase in protease, amylase and biosurfactant production. The proteolytic activity was found to be optimum at pH 9.0 and 60 °C while optimum amylolytic activity was recorded at pH 6.0 and 70 °C respectively. Both enzymes were found to be stable in the presence of organic solvents, ionic and commercial detergent and oxidizing agents. The biosurfactant was extracted with chloroform and was found to be stable at varying pH and temperature; however a reduction in the activity was observed at temperature higher than 70 °C. The isolated enzymes and biosurfactants may find applications in the effective removal of stains.

  1. Thermal Stability of LiPF6 Salt and Li-ion Battery Electrolytes Containing LiPF6

    OpenAIRE

    Yang, Hui; Zhuang, Guorong V.; Ross Jr., Philip N.

    2006-01-01

    The thermal stability of the neat LiPF6 salt and of 1 molal solutions of LiPF6 in prototypical Li-ion battery solvents was studied with thermogravimetric analysis (TGA) and on-line FTIR. Pure LiPF6 salt is thermally stable up to 380 oK in a dry inert atmosphere, and its decomposition path is a simple dissociation producing LiF as solid and PF5 as gaseous products. In the presence of water (300 ppm) in the carrier gas, its decomposition onset temperature is lowered as a result of direct t...

  2. Effect of failures and repairs on multiple cell production lines

    Energy Technology Data Exchange (ETDEWEB)

    Legato, P.; Bobbio, A.; Roberti, L.

    1989-01-01

    This paper examines a production line composed of multiple stages, or cells, which are passed in sequential order to arrive to the final product. Two possible coordination disciplines are considered, namely: the classical tandem arrangement of sequential working centers with input buffer and the kanban scheme, considered the Japanese shop floor realization of the Just-In-Time (JIT) manifacturing approach. The production line is modelled and analysed by means of Stochastic Petri Nets (SPN). Finally an analysis is made of the possibility that the working cells can incur failure/repair cycles perturbing the production flow of the line and thus reduce performance indices.

  3. Improving new product development (NPD process by analyzing failure cases

    Directory of Open Access Journals (Sweden)

    Yeon-Hak Kim

    2017-01-01

    Full Text Available Purpose - The purpose of this study is to develop an appropriate new product development (NPD process of Company “T”, a medium-sized firm, by analyzing the existing NPD process and failure cases of the Company. Design/methodology/approach - The proposed research framework is as follows: first, prospective studies of the NPD process are performed using the existing literature and preliminary references; second, comparative analysis between the current processes and a NPD process is performed; third, phase-based evaluations upon failed product cases are conducted with a NPD process so as to identify the abridged steps and root-causes of failures; finally, renewed priorities are set forth by utilizing the analytic hierarchy process analysis and questionnaire analysis upon the above identified causes of failures. Findings - The resulting accomplishments include the establishment of NPD processes that resonates with the current states of Company “T”, which, in turn, ensures the increase of efficiency, the decrease in development duration and the strategy of capacity-concentration and priority-selection. Originality/value - As Company “T”’s development process is outdated and products are developed without adequate market information research and feasibility analysis, the percentage of failed development project is as high as 87 per cent. Thus, this study aims to develop an appropriate NPD process of Company “T” by analyzing the existing NPD process and failure cases of the Company.

  4. Thermal stability of LiPF 6 salt and Li-ion battery electrolytes containing LiPF 6

    Science.gov (United States)

    Yang, Hui; Zhuang, Guorong V.; Ross, Philip N.

    The thermal stability of the neat lithium hexafluorophosphate (LiPF 6) salt and of 1 molal (m) solutions of LiPF 6 in prototypical Li-ion battery solvents was studied with thermogravimetric analysis (TGA) and on-line Fourier transform infrared (FTIR). Pure LiPF 6 salt is thermally stable up to 107 °C in a dry inert atmosphere, and its decomposition path is a simple dissociation producing lithium fluoride (LiF) as solid and PF 5 as gaseous products. In the presence of water (300 ppm) in the carrier gas, its decomposition onset temperature is lowered as a result of direct thermal reaction between LiPF 6 and water vapor to form phosphorous oxyfluoride (POF 3) and hydrofluoric acid (HF). No new products were observed in 1 m solutions of LiPF 6 in ethylene carbonate (EC), dimethyl carbonate (DMC) and ethyl methyl carbonate (EMC) by on-line TGA-FTIR analysis. The storage of the same solutions in sealed containers at 85 °C for 300-420 h did not produce any significant quantity of new products as well. In particular, no alkylflurophosphates were found in the solutions after storage at elevated temperature. In the absence of either an impurity like alcohol or cathode active material that may (or may not) act as a catalyst, there is no evidence of thermally induced reaction between LiPF 6 and the prototypical Li-ion battery solvents EC, PC, DMC or EMC.

  5. Study on the pyrolysis of phenol-formaldehyde (PF) resin and modified PF resin

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Jigang, E-mail: wangjigang@seu.edu.cn [Jiangsu Key Laboratory of Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing 211189 (China); Jiang, Haiyun [Jiangsu Key Laboratory of Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing 211189 (China); School of Materials Science and Engineering, Southeast University, Nanjing Institute of Technology, Nanjing 210013 (China); Jiang, Nan [Institute of Theoretical and Computational Chemistry, Nanjing University, Nanjing 210093 (China)

    2009-12-10

    The pyrolysis of pure phenol-formaldehyde (PF) resin and boron carbide (B{sub 4}C) modified PF resin was investigated by using thermogravimetry (TG) and pyrolysis gas-chromatography-mass-spectrometry (PY-GC/MS). Scanning electron microscope (SEM) and Fourier transform infrared (FT-IR) spectroscopy were also employed to investigate the micro-structural evolution. It was shown from the TG analysis that the char residues of pure PF resin were 62.9 and 60.5% after being pyrolyzed at 700 and 1000 {sup o}C, respectively. The degradation and failure of the resin matrix were mainly resulted from the release of volatiles. The phenol and its methyl derivates took a large proportion in the amount of volatiles. In comparison with the pure PF resin, the char residues of B{sub 4}C modified PF resin were obviously higher, with the values of 71.9 and 68.4% at 700 and 1000 {sup o}C, respectively. Due to the oxidation-reduction reactions between B{sub 4}C additive and oxygen-containing volatiles including CO and H{sub 2}O, partial carbon and oxygen elements in the volatiles remained in the resin matrix in the forms of amorphous carbon and B{sub 2}O{sub 3}, respectively. The results of SEM and FT-IR characterization demonstrated the occurrence of the modification, and the amorphous carbon existed in the form of reticular substance. In addition, the amount of the released phenol and its methyl derivates was also decreased drastically due to the formation of borate.

  6. Synthesis, characterization of a new photosensitive compound [Ru(bpy)2 (TPAD)](PF6)2 and its application for photocatalytic hydrogen production

    Science.gov (United States)

    Zheng, Hui-Qin; Guo, Yi-Ping; Yin, Ming-Cai; Fan, Yao-Ting

    2016-06-01

    A new photosensitive compound based on triphenylamine donor and ruthenium(II), namely [Ru(bpy)2(TPAD)](PF6)2 (PS1, bpy = 2,2‧-bipyridine, TPAD = N-(4-(1H-imidazo[4,5-f][1,10] phenanthroline-2-yl)phenyl)-N-phenylbenzenamine), had been synthesized and characterized by elemental analysis, 1H NMR, MS spectra, UV, PL and CV measurements. The target photocatalyst, PS1/0.5 wt% Pt/TiO2, was prepared by sensitizing PS1 to 0.5 wt% Pt/TiO2 and characterized by SEM and XRD. The highest hydrogen evolution reached to 9500 μmol·(g·catalyst)-1 (475 μmol, 31.7 TON and TOF 5.8 vs. PS1) under the optimal conditions with 0.05 g·L-1 of PS1, and 5% of TEOA at pH 11 with the irradiation of 5.5 h. The mechanism of hydrogen evolution was also briefly discussed in the present work.

  7. Failures of knowledge production in nuclear power risk management

    International Nuclear Information System (INIS)

    Sanne, Johan M.

    2008-09-01

    Risks are ascribed in processes of knowledge production, where risk objects are defined and measures taken. This knowledge is also the basis for regulatory action. Thus, uncertainties in knowledge production, based upon choices of assumptions, methods, calculations and evidence criteria for reliable data create vulnerabilities for risk management and risk regulation. A recent incident in Swedish nuclear power plant provides an opportunity to develop theories of knowledge production in complex organizations. Knowledge modes within nuclear power can be characterized as either calculated logics where evidence claims need numbers, real time logics based upon subtle signals and tacit knowledge or as policy logics, navigating between internal and external demands for safety, trustworthiness and profit. The plant had neither foreseen the triggering event nor designed the plant to withstand it. I analyze how the plant and the regulator have interpreted the event, its significance and the measures taken to prevent similar events. I also discuss alternative interpretations, lack of knowledge and the generic deficiencies in knowledge production that the event indicates. First, the plant was not as robustly designed as expected. Deficiencies in diversification may have been caused by overconfidence in the reliability of its design. Second, inadequate design was ascribed to various deficient knowledge production processes: original design of the plant, reconstruction or caused by inadequate learning from previous events. The failures in knowledge production were probably caused by insufficient integration of different knowledge processes and limitations in engineering analysis. Knowledge about risks from nuclear power operations is mainly based upon calculations and simulations, not upon real events. But knowledge and design could be improved also without accidents. Control room operators and maintenance staff can provide invaluable knowledge and methods; to improve causal

  8. Failures of knowledge production in nuclear power risk management

    Energy Technology Data Exchange (ETDEWEB)

    Sanne, Johan M.

    2008-09-15

    Risks are ascribed in processes of knowledge production, where risk objects are defined and measures taken. This knowledge is also the basis for regulatory action. Thus, uncertainties in knowledge production, based upon choices of assumptions, methods, calculations and evidence criteria for reliable data create vulnerabilities for risk management and risk regulation. A recent incident in Swedish nuclear power plant provides an opportunity to develop theories of knowledge production in complex organizations. Knowledge modes within nuclear power can be characterized as either calculated logics where evidence claims need numbers, real time logics based upon subtle signals and tacit knowledge or as policy logics, navigating between internal and external demands for safety, trustworthiness and profit. The plant had neither foreseen the triggering event nor designed the plant to withstand it. I analyze how the plant and the regulator have interpreted the event, its significance and the measures taken to prevent similar events. I also discuss alternative interpretations, lack of knowledge and the generic deficiencies in knowledge production that the event indicates. First, the plant was not as robustly designed as expected. Deficiencies in diversification may have been caused by overconfidence in the reliability of its design. Second, inadequate design was ascribed to various deficient knowledge production processes: original design of the plant, reconstruction or caused by inadequate learning from previous events. The failures in knowledge production were probably caused by insufficient integration of different knowledge processes and limitations in engineering analysis. Knowledge about risks from nuclear power operations is mainly based upon calculations and simulations, not upon real events. But knowledge and design could be improved also without accidents. Control room operators and maintenance staff can provide invaluable knowledge and methods; to improve causal

  9. TPX Poloidal Field (PF) power systems simulation

    International Nuclear Information System (INIS)

    Lu, E.; Bronner, G.

    1993-01-01

    This paper describes the modeling and simulation of the PF power system for the Tokamak Physics Experiment (TPX), which is required to supply pulsed DC current to the Poloidal Field (PF) superconducting coil system. An analytical model was developed to simulate the dynamics of the PF power system for any PF current scenario and thereby provide the basis for selection of PF circuit topology, in support of the major design goal of optimizing the use of the existing Tokamak Fusion Test Reactor (TFTR) facilities at the Princeton Plasma Physics Lab (PPPL)

  10. Genomics-guided discovery of secondary metabolites and their regulation in Pseudomonas protegens Pf-5

    Science.gov (United States)

    Pseudomonas protegens strain Pf-5 is a well-characterized rhizosphere bacterium known for its production of a diverse spectrum of secondary metabolites and its capacity to suppress plant diseases caused by soilborne fungal, bacterial and oomycete pathogens. Metabolites produced by Pf-5 include 2,4-...

  11. The rare codon AGA is involved in regulation of pyoluteorin biosynthesis in Pseudomonas protegens Pf-5

    Science.gov (United States)

    The soil bacterium Pseudomonas protegens Pf-5 can colonize root and seed surfaces of many plants, protecting them from infection by plant pathogenic fungi and oomycetes. This capacity to suppress disease is attributed in part to Pf-5’s production of a large spectrum of antibiotics, which is controll...

  12. ITER PF6 double pancakes winding line

    Energy Technology Data Exchange (ETDEWEB)

    Du, Shuangsong [Institute of Plasma Physics, Chinese Academy of Science, Hefei (China); University of Science and Technology of China, Hefei (China); Wen, Wei, E-mail: wenwei@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Science, Hefei (China); Chen, Jin; Wu, Weiyue; Song, Yuntao; Shen, Guang [Institute of Plasma Physics, Chinese Academy of Science, Hefei (China)

    2017-03-15

    Highlights: • ITER PF6 double pancakes “two-in-hand” winding line layout and main parameters were introduced, main winding sequences were also included. • Main features of each winding unit include de-spooling unit, straightening unit, sandblasting and cleaning unit, bending unit, turn insulation wrapping head, rotary table and automatic control system were depicted. • PF6 double pancake winding line was commissioned with PF5 empty jacket conductor after the installation and testing of each unit, ±0.5 mm turn positioning and ±2 turn to turn deviations were achieved. - Abstract: The Poloidal Field (PF) coils are one of the main sub-systems of the ITER magnets. The PF6 coil is being manufactured by the Institute of Plasma Physics, Chinese Academy of Sciences (ASIPP) as per the Poloidal Field coils cooperation agreement signed between ASIPP and Fusion for Energy (F4E).The ITER PF6 winding pack is composed by stacking of 9 double pancakes. Each double pancake is wound with a “two-in-hand” configuration. This paper describes the ITER PF6 double pancakes winding line, including layout and main parameters of the winding line, features of main units and the commissioning trial with PF5 empty jacket conductor.

  13. Status and performance of PF injector linac

    International Nuclear Information System (INIS)

    Sato, Isamu

    1994-01-01

    PF injector linac has been improved on a buncher section for accelerating of intense electron beam, and reinforced a focusing system of the positron generator linac for the expansion of phase space. In this presentation, I shall report present status and performance of PF injector linac, and discuss its upgrade program for B-factory project. (author)

  14. Proposed kinetic mechanism of biodiesel production through lipase catalysed interesterification with a methyl acetate acyl acceptor and ionic liquid BMIM PF6 co-solvent

    Energy Technology Data Exchange (ETDEWEB)

    Ruzich, Nicholas Ivan; Bassi, A.S.

    2011-02-15

    As the depletion of fossil resources continues, the demand for environmentally friendly sources of energy as biodiesel is increasing. Biodiesel is the resulting fatty acid methyl ester (FAME) from an esterification reaction. To enhance this reaction lipase catalysts and ionic liquids can also used. The aim of this study was to develop a kinetic model of this reaction and to determine the effect of ionic liquids. The esterification reaction was conducted in a jacketed reactor under constant temperature in presence of ionic liquid and with the use of lipase to start the reaction; samples were analyzed with a high-performance liquid chromatography. A kinetic model was developed and results indicated that ionic liquids tend to limit mass transfer and thus reduce initial reaction rates; the authors proposed to increase the flow rate of the reaction mixture to overcome this issue. This study successfully investigated the reaction of lipase-catalyzed biodiesel production and developed its kinetic model.

  15. Application of multi attribute failure mode analysis of milk production using analytical hierarchy process method

    Science.gov (United States)

    Rucitra, A. L.

    2018-03-01

    Pusat Koperasi Induk Susu (PKIS) Sekar Tanjung, East Java is one of the modern dairy industries producing Ultra High Temperature (UHT) milk. A problem that often occurs in the production process in PKIS Sekar Tanjung is a mismatch between the production process and the predetermined standard. The purpose of applying Analytical Hierarchy Process (AHP) was to identify the most potential cause of failure in the milk production process. Multi Attribute Failure Mode Analysis (MAFMA) method was used to eliminate or reduce the possibility of failure when viewed from the failure causes. This method integrates the severity, occurrence, detection, and expected cost criteria obtained from depth interview with the head of the production department as an expert. The AHP approach was used to formulate the priority ranking of the cause of failure in the milk production process. At level 1, the severity has the highest weight of 0.41 or 41% compared to other criteria. While at level 2, identifying failure in the UHT milk production process, the most potential cause was the average mixing temperature of more than 70 °C which was higher than the standard temperature (≤70 ° C). This failure cause has a contributes weight of 0.47 or 47% of all criteria Therefore, this study suggested the company to control the mixing temperature to minimise or eliminate the failure in this process.

  16. Fission product concentration evolution in sodium pool following a fuel subassembly failure in an LMFBR

    International Nuclear Information System (INIS)

    Natesan, K.; Velusamy, K.; Selvaraj, P.; Kasinathan, N.; Chellapandi, P.; Chetal, S.; Bhoje, S.

    2003-01-01

    During a fuel element failure in a liquid metal cooled fast breeder reactor, the fission products originating from the failed pins mix into the sodium pool. Delayed Neutron Detectors (DND) are provided in the sodium pool to detect such failures by way of detection of delayed neutrons emitted by the fission products. The transient evolution of fission product concentration is governed by the sodium flow distribution in the pool. Transient hydraulic analysis has been carried out using the CFD code PHOENICS to estimate fission product concentration evolution in hot pool. k- ε turbulence model and zero laminar diffusivity for the fission product concentration have been considered in the analysis. Times at which the failures of various fuel subassemblies (SA) are detected by the DND are obtained. It has been found that in order to effectively detect the failure of every fuel SA, a minimum of 8 DND in hot pool are essential

  17. Measurement of fission product release during LWR fuel failure

    International Nuclear Information System (INIS)

    Osetek, D.J.; King, J.J.

    1979-01-01

    The PBF is a specialized test reactor consisting of an annular core and a central test space 21 cm in diameter and 91 cm high. A test loop circulates coolant through the central experimental section at typical power reactor conditions. Light-water-reactor-type fuel rods are exposed to power bursts simulating reactivity insertion transients, and to power-cooling-mismatch conditions during which the rods are allowed to operate in film boiling. Fission product concentrations in the test loop coolant are continuously monitored during these transients by a Ge(Li) detector based gamma spectrometer. Automatic batch processing of pulse height spectra results in a list of radionuclide concentrations present in the loop coolant as a function of time during the test. Fission product behavior is then correlated to test parameters and posttest examination of the fuel rods. Data are presented from Test PCM-1

  18. Dissection of the role of PfEMP1 and ICAM-1 in the sensing of Plasmodium-falciparum-infected erythrocytes by natural killer cells.

    Directory of Open Access Journals (Sweden)

    Myriam Baratin

    Full Text Available BACKGROUND: Host innate immunity contributes to malaria clinical outcome by providing protective inflammatory cytokines such as interferon-gamma, and by shaping the adaptive immune response. Plasmodium falciparum (Pf is the etiologic agent of the most severe forms of human malaria. Natural Killer (NK cells are lymphocytes of the innate immune system that are the first effectors to produce interferon-gamma in response to Pf. However, the molecular bases of Pf-NK cell recognition events are unknown. Our study focuses on the role of Pf erythrocyte membrane protein 1 (PfEMP1, a major Pf virulence factor. PfEMP1 is expressed on parasitized-erythrocytes and participates to vascular obstruction through the binding to several host receptors. PfEMP1 is also a pivotal target for host antibody response to Pf infection. METHODOLOGY/PRINCIPAL FINDINGS: Using genetically-engineered parasite mutant strains, a human genetic deficiency, and blocking antibodies, we identified two receptor-ligand pairs involved in two uncoupled events occurring during the sensing of Pf infection by NK cells. First, PfEMP1 interaction with one of its host receptor, chondroitin sulfate A, mediates the cytoadhesion of Pf-infected erythrocytes to human NK cell lines, but is not required for primary NK cell activation. Second, intercellular adhesion molecule-1 (ICAM-1, another host receptor for PfEMP1, is mandatory for NK cell interferon-gamma response. In this case, ICAM-1 acts via its engagement with its host ligand, LFA-1, and not with PfEMP1, consistent with the obligatory cross-talk of NK cells with macrophages for their production of interferon-gamma. CONCLUSION/SIGNIFICANCE: PfEMP1-independent but ICAM-1/LFA-1-dependent events occurring during NK cell activation by Pf highlight the fundamental role of cellular cooperation during innate immune response to malaria.

  19. Fission product release modelling for application of fuel-failure monitoring and detection - An overview

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, B.J., E-mail: lewibre@gmail.com [Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, K7K 7B4 (Canada); Chan, P.K.; El-Jaby, A. [Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, K7K 7B4 (Canada); Iglesias, F.C.; Fitchett, A. [Candesco Division of Kinectrics Inc., 26 Wellington Street East, 3rd Floor, Toronto, Ontario M5E 1S2 (Canada)

    2017-06-15

    A review of fission product release theory is presented in support of fuel-failure monitoring analysis for the characterization and location of defective fuel. This work is used to describe: (i) the development of the steady-state Visual-DETECT code for coolant activity analysis to characterize failures in the core and the amount of tramp uranium; (ii) a generalization of this model in the STAR code for prediction of the time-dependent release of iodine and noble gas fission products to the coolant during reactor start-up, steady-state, shutdown, and bundle-shifting manoeuvres; (iii) an extension of the model to account for the release of fission products that are delayed-neutron precursors for assessment of fuel-failure location; and (iv) a simplification of the steady-state model to assess the methodology proposed by WANO for a fuel reliability indicator for water-cooled reactors.

  20. Failure criterion effect on solid production prediction and selection of completion solution

    Directory of Open Access Journals (Sweden)

    Dariush Javani

    2017-12-01

    Full Text Available Production of fines together with reservoir fluid is called solid production. It varies from a few grams or less per ton of reservoir fluid posing only minor problems, to catastrophic amount possibly leading to erosion and complete filling of the borehole. This paper assesses solid production potential in a carbonate gas reservoir located in the south of Iran. Petrophysical logs obtained from the vertical well were employed to construct mechanical earth model. Then, two failure criteria, i.e. Mohr–Coulomb and Mogi–Coulomb, were used to investigate the potential of solid production of the well in the initial and depleted conditions of the reservoir. Using these two criteria, we estimated critical collapse pressure and compared them to the reservoir pressure. Solid production occurs if collapse pressure is greater than pore pressure. Results indicate that the two failure criteria show different estimations of solid production potential of the studied reservoir. Mohr–Coulomb failure criterion estimated solid production in both initial and depleted conditions, where Mogi–Coulomb criterion predicted no solid production in the initial condition of reservoir. Based on Mogi–Coulomb criterion, the well may not require completion solutions like perforated liner, until at least 60% of reservoir pressure was depleted which leads to decrease in operation cost and time.

  1. DENA, a new PF device

    International Nuclear Information System (INIS)

    Tafreshi, M.A.; Farrahi, M.; Lamehi, M.

    2001-01-01

    In this paper we are going to introduce 'Dena', a new Filippov type plasma focus facility, with a condenser bank of 0.288 mF, and a maximum supplying energy of 90 kJ (at V max = 25 kV). The facility was installed and started to work in the first quarter of the year 2000. Major points of the paper are: - Dena's construction, functionality, and diagnostic system. - Preliminary results: Different modes of neutron, X-ray, and 'hot spot' production, as well as the possibility of controlling operational mode, by changing anode configuration and initial discharge condition. (author)

  2. A Further Study of Productive Failure in Mathematical Problem Solving: Unpacking the Design Components

    Science.gov (United States)

    Kapur, Manu

    2011-01-01

    This paper replicates and extends my earlier work on productive failure in mathematical problem solving (Kapur, doi:10.1007/s11251-009-9093-x, 2009). One hundred and nine, seventh-grade mathematics students taught by the same teacher from a Singapore school experienced one of three learning designs: (a) traditional lecture and practice (LP), (b)…

  3. How to Flip the Classroom--"Productive Failure or Traditional Flipped Classroom" Pedagogical Design?

    Science.gov (United States)

    Song, Yanjie; Kapur, Manu

    2017-01-01

    The paper reports a quasi-experimental study comparing the "traditional flipped classroom" pedagogical design with the "productive failure" (Kapur, 2016) pedagogical design in the flipped classroom for a 2-week curricular unit on polynomials in a Hong Kong Secondary school. Different from the flipped classroom where students…

  4. Classification and calculation of primary failure modes in bread production line

    International Nuclear Information System (INIS)

    Tsarouhas, Panagiotis H.

    2009-01-01

    In this study, we describe the classification methodology over a 2-year period of the primary failure modes in categories based on failure data of bread production line. We estimate the probabilities of these categories applying the chi-square goodness of fit test, and we calculate their joint probabilities of mass function at workstation and line level. Then, we present numerical examples in order to predict the causes and frequencies of breakdowns for workstations and for the entire bread production line that will occur in the future. The methodology is meant to guide bread and bakery product manufacturers, improving the operation of the production lines. It can also be a useful tool to maintenance engineers, who wish to analyze and improve the reliability and efficiency of the manufacturing systems

  5. Failure mode and effects analysis in a dual-product microsphere brachytherapy environment.

    Science.gov (United States)

    Younge, Kelly Cooper; Lee, Choonik; Moran, Jean M; Feng, Mary; Novelli, Paula; Prisciandaro, Joann I

    We performed a failure mode and effects analysis (FMEA) during the addition of a new microspheres product into our existing microsphere brachytherapy program to identify areas for safety improvements. A diverse group of team members from the microsphere program participated in the project to create a process map, identify and score failure modes, and discuss programmatic changes to address the highest ranking items. We developed custom severity ranking scales for staff- and institution-related failure modes to encompass possible risks that may exist outside of patient-based effects. Between both types of microsphere products, 173 failure mode/effect pairs were identified: 90 for patients, 35 for staff, and 48 for the institution. The SIR-Spheres program was ranked separately from the TheraSphere program because of significant differences in workflow during dose calculation, preparation, and delivery. High-ranking failure modes in each category were addressed with programmatic changes. The FMEA aided in identifying potential risk factors in our microsphere program and allowed a theoretically safer and more efficient design of the workflow and quality assurance for both our new SIR-Spheres program and our existing TheraSphere program. As new guidelines are made available, and our experience with the SIR-Spheres program increases, we will update the FMEA as an efficient starting point for future improvements. Copyright © 2016 American Society for Radiation Oncology. Published by Elsevier Inc. All rights reserved.

  6. Operational Status of PF-Ring and PF-AR after the Earthquake

    International Nuclear Information System (INIS)

    Honda, T; Asaoka, S; Haga, K; Harada, K; Honda, Y; Izawa, M; Kamiya, Y; Kobayashi, Y; Miyajima, T; Miyauchi, H; Nagahashi, S; Nakamura, N; Nogami, T; Obina, T; Ozaki, T; Sagehashi, H; Sakai, H; Sakanaka, S; Sasaki, H; Sato, Y

    2013-01-01

    In 2011, two SR sources of KEK, PF-ring and PF-AR, needed to change the operation schedule because of the unprecedented earthquake on March 11. Though the injector linac and the storage rings suffered a serious damage, temporary recovery was accomplished quickly and the trial operation started in May. The regular user operation could be resumed in October 2011. In the restoration work after the earthquake, some old vacuum components were removed from PF-ring. This work fortunately brought an effect of settling the quadrupole-mode longitudinal instability. For the top-up injection of PF-ring, the pulsed sextupole magnet has been used instead of the conventional kicker magnets since 2011. The hybrid-fill mode in place of the single-bunch mode has become available. Recently, the 10-Hz orbit switching for the tandem circularly polarized undulators has been developed for the user operation.

  7. Integrating Preventive Maintenance Scheduling As Probability Machine Failure And Batch Production Scheduling

    Directory of Open Access Journals (Sweden)

    Zahedi Zahedi

    2016-06-01

    Full Text Available This paper discusses integrated model of batch production scheduling and machine maintenance scheduling. Batch production scheduling uses minimize total actual flow time criteria and machine maintenance scheduling uses the probability of machine failure based on Weibull distribution. The model assumed no nonconforming parts in a planning horizon. The model shows an increase in the number of the batch (length of production run up to a certain limit will minimize the total actual flow time. Meanwhile, an increase in the length of production run will implicate an increase in the number of PM. An example was given to show how the model and algorithm work.

  8. Optimization of a Multi-Product Intra-Supply Chain System with Failure in Rework.

    Science.gov (United States)

    Chiu, Singa Wang; Chen, Shin-Wei; Chang, Chih-Kai; Chiu, Yuan-Shyi Peter

    2016-01-01

    Globalization has created tremendous opportunities, but also made business environment highly competitive and turbulent. To gain competitive advantage, management of present-day transnational firms always seeks options to trim down various transaction and coordination costs, especially in the area of controllable intra-supply chain system. This study investigates a multi-product intra-supply chain system with failure in rework. To achieve maximum machine utilization, multiple products are fabricated in succession on a single machine. During the process, production of some defective items is inevitable. Reworking of nonconforming items is used to reduce the quality cost in production and achieving the goal of lower overall production cost. Because reworks are sometimes unsuccessful, failures in rework are also considered in this study. Finished goods for each product are transported to the sales offices when the entire production lot is quality assured after rework. A multi-delivery policy is used, wherein fixed quantity n installments of the finished lot are transported at fixed intervals during delivery time. The objective is to jointly determine the common production cycle time and the number of deliveries needed to minimize the long-term expected production-inventory-delivery costs for the problem. With the help of a mathematical model along with optimization technique, the optimal production-shipment policy is obtained. We have used a numerical example to demonstrate applicability of the result of our research.

  9. PF4-HIT antibody (KKO) complexes activate broad innate immune and inflammatory responses.

    Science.gov (United States)

    Haile, Lydia A; Rao, Roshni; Polumuri, Swamy K; Arepally, Gowthami M; Keire, David A; Verthelyi, Daniela; Sommers, Cynthia D

    2017-11-01

    Heparin-induced thrombocytopenia (HIT) is an immune-mediated complication of heparin anticoagulation therapy resulting in thrombocytopenia frequently accompanied by thrombosis. Current evidence suggests that HIT is associated with antibodies developed in response to multi-molecular complexes formed by platelet factor 4 (PF4) bound to heparin or cell surface glycosaminoglycans. These antibody complexes activate platelets and monocytes typically through FcγRIIA receptors increasing the production of PF4, inflammatory mediators, tissue factor and thrombin. The influence of underlying events in HIT including complex-induced pro-inflammatory cell activation and structural determinants leading to local inflammatory responses are not fully understood. The stoichiometry and complex component requirements were determined by incubating fresh peripheral blood mononuclear cells (PBMC) with different concentrations of unfractionated heparin (H), low molecular weight heparin (LMWH), PF4- and anti-PF4-H complex antibodies (KKO). Cytokine mRNA or protein were measured by qRT-PCR or Meso Scale Discovery technology, respectively. Gene expression profile analysis for 594 genes was performed using Nanostring technology and analyzed using Ingenuity Pathway Analysis software. The data show that antibodies magnify immune responses induced in PBMCs by PF4 alone or in complex with heparin or LMWH. We propose that following induction of HIT antibodies by heparin-PF4 complexes, binding of the antibodies to PF4 is sufficient to induce a local pro-inflammatory response which may play a role in the progression of HIT. In vitro assays using PBMCs may be useful in characterizing local inflammatory and innate immune responses induced by HIT antibodies in the presence of PF4 and different sources of heparins. The findings and conclusions in this article are solely the responsibility of the authors and are not being formally disseminated by the Food and Drug Administration. Thus, they should not be

  10. Detection of gaseous fission products in water - a method of monitoring fuel sheathing failures

    Energy Technology Data Exchange (ETDEWEB)

    Tunnicliffe, P. R.; Whittier, A. C.

    1959-05-15

    The gaseous activities stripped from samples of effluent coolant from the NRU fuel elements tested in the central thimble of the NRX reactor (NRU loop) and from the NRX main effluent have been investigated. The activities obtained from the NRU loop can be attributed to gaseous fission products only. Design data have been obtained for a 'Gaseous Fission Product Monitor' to be installed for use with the NRU reactor. It is expected that this monitor will have high sensitivity to activity indicative of an incipient fuel element sheath failure. No qualitative determination of the various gaseous activities obtained from the NRX effluent has been made. A strong component of 25 {+-}1 seconds half-life is not consistent with O-19. Limited information concerning sheath failures in NRX was obtained. Of six failures observed in parallel with the installed delayed neutron monitors, three of these gave pre-warnings and in each case the gaseous fission product monitor showed a substantially greater sensitivity. An experiment in which small samples of uranium, inserted into the NRX reactor, could be exposed at will to a stream of water showed the behaviour of the two types of monitors to be similar. However, a number of signals were detected only by the gaseous fission product monitor. These can be attributed to its sensitivity to relatively long lived fission products. (author)

  11. Optimization of a Multi–Product Intra-Supply Chain System with Failure in Rework

    Science.gov (United States)

    2016-01-01

    Globalization has created tremendous opportunities, but also made business environment highly competitive and turbulent. To gain competitive advantage, management of present-day transnational firms always seeks options to trim down various transaction and coordination costs, especially in the area of controllable intra-supply chain system. This study investigates a multi–product intra-supply chain system with failure in rework. To achieve maximum machine utilization, multiple products are fabricated in succession on a single machine. During the process, production of some defective items is inevitable. Reworking of nonconforming items is used to reduce the quality cost in production and achieving the goal of lower overall production cost. Because reworks are sometimes unsuccessful, failures in rework are also considered in this study. Finished goods for each product are transported to the sales offices when the entire production lot is quality assured after rework. A multi-delivery policy is used, wherein fixed quantity n installments of the finished lot are transported at fixed intervals during delivery time. The objective is to jointly determine the common production cycle time and the number of deliveries needed to minimize the long–term expected production–inventory–delivery costs for the problem. With the help of a mathematical model along with optimization technique, the optimal production–shipment policy is obtained. We have used a numerical example to demonstrate applicability of the result of our research. PMID:27918588

  12. Electron-capture Rates for pf-shell Nuclei in Stellar Environments and Nucleosynthesis

    Science.gov (United States)

    Suzuki, Toshio; Honma, Michio; Mori, Kanji; Famiano, Michael A.; Kajino, Toshitaka; Hidakai, Jun; Otsuka, Takaharu

    Gamow-Teller strengths in pf-shell nuclei obtained by a new shell-model Hamltonian, GXPF1J, are used to evaluate electron-capture rates in pf-shell nuclei at stellar environments. The nuclear weak rates with GXPF1J, which are generally smaller than previous evaluations for proton-rich nuclei, are applied to nucleosynthesis in type Ia supernova explosions. The updated rates are found to lead to less production of neutron-rich nuclei such as 58Ni and 54Cr, thus toward a solution of the problem of over-production of neutron-rich isotopes of iron-group nuclei compared to the solar abundance.

  13. Klotho expression in long bones regulates FGF23 production during renal failure.

    Science.gov (United States)

    Kaludjerovic, Jovana; Komaba, Hirotaka; Sato, Tadatoshi; Erben, Reinhold G; Baron, Roland; Olauson, Hannes; Larsson, Tobias E; Lanske, Beate

    2017-05-01

    Circulating levels of bone-derived fibroblast growth factor 23 (FGF23) increase early during acute and chronic kidney disease and are associated with adverse outcomes. Membrane-bound Klotho acts as a permissive coreceptor for FGF23, and its expression was recently found in osteoblasts/osteocytes. We hypothesized that Klotho in bone cells is part of an autocrine feedback loop that regulates FGF23 expression during renal failure. Thus, we induced renal failure in mice with targeted deletion of Klotho in long bones. Uremic wild-type ( KL fl/fl ) and knockout ( Prx1-Cre;KL fl/fl ) mice both responded with reduced body weight, kidney atrophy, hyperphosphatemia, and increased bone turnover. Importantly, long bones of Prx1-Cre;KL fl/fl mice but not their axial skeleton failed to increase FGF23 expression as observed in uremic KL fl/fl mice. Consequently, Prx1-Cre;KL fl/fl mice had significantly lower serum FGF23 and parathyroid hormone levels, and higher renal 1-α-hydroxylase expression, serum 1,25-dihydroxyvitamin D, and calcium levels than KL fl/fl mice. These results were confirmed in two independent models of renal failure, adenine diet induced and 5/6 nephrectomy. Moreover, FGF23-treated bone cells required Klotho to increase FGF23 mRNA and ERK phosphorylation. In summary, our novel findings show that Klotho in bone is crucial for inducing FGF23 production upon renal failure. We propose the presence of an autocrine feedback loop in which Klotho senses the need for FGF23.-Kaludjerovic, J., Komaba, H., Sato, T., Erben, R. G., Baron, R., Olauson, H., Larsson, T. E., Lanske, B. Klotho expression in long bones regulates FGF23 production during renal failure. © FASEB.

  14. 16 CFR 1115.5 - Reporting of failures to comply with a voluntary consumer product safety standard relied upon by...

    Science.gov (United States)

    2010-01-01

    ... voluntary consumer product safety standard relied upon by the Commission under section 9 of the CPSA. 1115.5 Section 1115.5 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS SUBSTANTIAL PRODUCT HAZARD REPORTS General Interpretation § 1115.5 Reporting of failures to comply...

  15. Failures to further developing orphan medicinal products after designation granted in Europe: an analysis of marketing authorisation failures and abandoned drugs.

    Science.gov (United States)

    Giannuzzi, Viviana; Landi, Annalisa; Bosone, Enrico; Giannuzzi, Floriana; Nicotri, Stefano; Torrent-Farnell, Josep; Bonifazi, Fedele; Felisi, Mariagrazia; Bonifazi, Donato; Ceci, Adriana

    2017-09-11

    The research and development process in the field of rare diseases is characterised by many well-known difficulties, and a large percentage of orphan medicinal products do not reach the marketing approval.This work aims at identifying orphan medicinal products that failed the developmental process and investigating reasons for and possible factors influencing failures. Drugs designated in Europe under Regulation (European Commission) 141/2000 in the period 2000-2012 were investigated in terms of the following failures: (1) marketing authorisation failures (refused or withdrawn) and (2) drugs abandoned by sponsors during development.Possible risk factors for failure were analysed using statistically validated methods. This study points out that 437 out of 788 designations are still under development, while 219 failed the developmental process. Among the latter, 34 failed the marketing authorisation process and 185 were abandoned during the developmental process. In the first group of drugs (marketing authorisation failures), 50% reached phase II, 47% reached phase III and 3% reached phase I, while in the second group (abandoned drugs), the majority of orphan medicinal products apparently never started the development process, since no data on 48.1% of them were published and the 3.2% did not progress beyond the non-clinical stage.The reasons for failures of marketing authorisation were: efficacy/safety issues (26), insufficient data (12), quality issues (7), regulatory issues on trials (4) and commercial reasons (1). The main causes for abandoned drugs were efficacy/safety issues (reported in 54 cases), inactive companies (25.4%), change of company strategy (8.1%) and drug competition (10.8%). No information concerning reasons for failure was available for 23.2% of the analysed products. This analysis shows that failures occurred in 27.8% of all designations granted in Europe, the main reasons being safety and efficacy issues. Moreover, the stage of development

  16. Accident management to prevent containment failure and reduce fission product release

    International Nuclear Information System (INIS)

    Lehner, J.R.; Lin, C.C.; Luckas, W.J.; Pratt, W.T.

    1991-01-01

    Brookhaven National Laboratory, under the auspices of the US Nuclear Regulatory Commission, is investigating accident management strategies which could help preserve containment integrity or minimize releases during a severe accident. The strategies considered make use of existing plant systems and equipment in innovative ways to reduce the likelihood of containment failure or to mitigate the release of fission products to the environment if failure cannot be prevented. Many of these strategies would be implemented during the later stages of a severe accident, i.e. after vessel breach, and sizable uncertainties exist regarding some of the phenomena involved. The identification and assessment process for containment and release strategies is described, and some insights derived from its application to specific containment types are presented. 2 refs., 5 figs., 2 tabs

  17. Engineering Pseudomonas protegens Pf-5 for Nitrogen Fixation and its Application to Improve Plant Growth under Nitrogen-Deficient Conditions

    Science.gov (United States)

    Setten, Lorena; Soto, Gabriela; Mozzicafreddo, Matteo; Fox, Ana Romina; Lisi, Christian; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba; Díaz-Paleo, Antonio; Ayub, Nicolás Daniel

    2013-01-01

    Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops. PMID:23675499

  18. Hardware-in-the-loop simulation of the EAST PF converter for PF control system upgrade

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Xiaojiao, E-mail: chenxj@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Science (ASIPP), P.O. Box 1126, Hefei, Anhui Province 230031 (China); Chinese University of Science and Technology, Hefei, Anhui Province 230022 (China); Fu, Peng [Institute of Plasma Physics, Chinese Academy of Science (ASIPP), P.O. Box 1126, Hefei, Anhui Province 230031 (China); Chinese University of Science and Technology, Hefei, Anhui Province 230022 (China); Huang, Liansheng, E-mail: huangls@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Science (ASIPP), P.O. Box 1126, Hefei, Anhui Province 230031 (China); Gao, Ge; He, Shiying [Institute of Plasma Physics, Chinese Academy of Science (ASIPP), P.O. Box 1126, Hefei, Anhui Province 230031 (China)

    2016-11-15

    Highlights: • The hardware in the loop simulation of the EAST PF system is presented. • The control functions and the protection logic have been tested and verified. • The major faults could be avoided and commissioning time could be saved on site. - Abstract: The EAST poloidal field (PF) control system was upgraded in 2015 and the new system has been in use for the 2015 EAST campaign. This paper presents the implementation of a hardware-in-the-loop (HIL) simulation platform of the EAST PF converter system based on the RT-LAB simulation environment, which was used to improve and evaluate the performance of the real controller. The EAST PF power supply system and its operational modes are presented in this paper. The experiments on HIL simulation platform show that the control algorithms and the over current protection of the controller meet the design requirements well. In addition, the effectiveness of the designed control system has been verified by actual application during the EAST campaign at 2015 for six months.

  19. Hardware-in-the-loop simulation of the EAST PF converter for PF control system upgrade

    International Nuclear Information System (INIS)

    Chen, Xiaojiao; Fu, Peng; Huang, Liansheng; Gao, Ge; He, Shiying

    2016-01-01

    Highlights: • The hardware in the loop simulation of the EAST PF system is presented. • The control functions and the protection logic have been tested and verified. • The major faults could be avoided and commissioning time could be saved on site. - Abstract: The EAST poloidal field (PF) control system was upgraded in 2015 and the new system has been in use for the 2015 EAST campaign. This paper presents the implementation of a hardware-in-the-loop (HIL) simulation platform of the EAST PF converter system based on the RT-LAB simulation environment, which was used to improve and evaluate the performance of the real controller. The EAST PF power supply system and its operational modes are presented in this paper. The experiments on HIL simulation platform show that the control algorithms and the over current protection of the controller meet the design requirements well. In addition, the effectiveness of the designed control system has been verified by actual application during the EAST campaign at 2015 for six months.

  20. A study of success and failure in product innovation with specific reference to the South African electronics industry

    OpenAIRE

    2014-01-01

    M.Com. (Business Management) The innovation and marketing of new products has become increasingly important for the profitability of companies. Developing new products is a risky endeavour. Research shows that new product development often fails. As such, it is very important that product development managers are aware of the factors that can cause product failure. This research studied the product innovation process in the South African electronics industry. The objective of the study was...

  1. An FMS Dynamic Production Scheduling Algorithm Considering Cutting Tool Failure and Cutting Tool Life

    International Nuclear Information System (INIS)

    Setiawan, A; Wangsaputra, R; Halim, A H; Martawirya, Y Y

    2016-01-01

    This paper deals with Flexible Manufacturing System (FMS) production rescheduling due to unavailability of cutting tools caused either of cutting tool failure or life time limit. The FMS consists of parallel identical machines integrated with an automatic material handling system and it runs fully automatically. Each machine has a same cutting tool configuration that consists of different geometrical cutting tool types on each tool magazine. The job usually takes two stages. Each stage has sequential operations allocated to machines considering the cutting tool life. In the real situation, the cutting tool can fail before the cutting tool life is reached. The objective in this paper is to develop a dynamic scheduling algorithm when a cutting tool is broken during unmanned and a rescheduling needed. The algorithm consists of four steps. The first step is generating initial schedule, the second step is determination the cutting tool failure time, the third step is determination of system status at cutting tool failure time and the fourth step is the rescheduling for unfinished jobs. The approaches to solve the problem are complete-reactive scheduling and robust-proactive scheduling. The new schedules result differences starting time and completion time of each operations from the initial schedule. (paper)

  2. Low Carbon-Oriented Optimal Reliability Design with Interval Product Failure Analysis and Grey Correlation Analysis

    Directory of Open Access Journals (Sweden)

    Yixiong Feng

    2017-03-01

    Full Text Available The problem of large amounts of carbon emissions causes wide concern across the world, and it has become a serious threat to the sustainable development of the manufacturing industry. The intensive research into technologies and methodologies for green product design has significant theoretical meaning and practical value in reducing the emissions of the manufacturing industry. Therefore, a low carbon-oriented product reliability optimal design model is proposed in this paper: (1 The related expert evaluation information was prepared in interval numbers; (2 An improved product failure analysis considering the uncertain carbon emissions of the subsystem was performed to obtain the subsystem weight taking the carbon emissions into consideration. The interval grey correlation analysis was conducted to obtain the subsystem weight taking the uncertain correlations inside the product into consideration. Using the above two kinds of subsystem weights and different caution indicators of the decision maker, a series of product reliability design schemes is available; (3 The interval-valued intuitionistic fuzzy sets (IVIFSs were employed to select the optimal reliability and optimal design scheme based on three attributes, namely, low carbon, correlation and functions, and economic cost. The case study of a vertical CNC lathe proves the superiority and rationality of the proposed method.

  3. TPX: Contractor preliminary design review. Volume 2, PF systems engineering

    International Nuclear Information System (INIS)

    Calvin, H.A.

    1995-01-01

    This system development specification covers the Poloidal Field (PF) Magnet System, WBS 14 in the Princeton Plasma Physics Laboratory TPX Program to build a tokamak fusion reactor. This specification establishes the performance, design, development and test requirements of the PF Magnet System

  4. TPX: Contractor preliminary design review. Volume 2, PF systems engineering

    Energy Technology Data Exchange (ETDEWEB)

    Calvin, H.A. [Lawrence Livermore National Lab., CA (United States)

    1995-07-28

    This system development specification covers the Poloidal Field (PF) Magnet System, WBS 14 in the Princeton Plasma Physics Laboratory TPX Program to build a tokamak fusion reactor. This specification establishes the performance, design, development and test requirements of the PF Magnet System.

  5. Application of cleaner production tools and failure modes and effects analysis in pig slaughterhourses

    Directory of Open Access Journals (Sweden)

    J. M. Fonseca

    2017-07-01

    Full Text Available Cleaner production programs (CP and Failure Modes and Effects Analysis (FMEA are tools used to improve the sustainability of industries, ensuring greater profitability, quality, reliability and safety of their products and services. The meat industry is among the most polluting industries because of the large amounts of organic waste produced during meat processing. The objective of this study was to combine the CP and FMEA tools and to apply them in a pig slaughterhouse in order to detect critical points along the production chain that have a major environmental impact and to establish corrective and preventive actions that could minimize these problems. The results showed that water is the most consumed resource by the industry and also the main producer of waste due to microbiological contamination with animal feces and blood and meat residues. All impacts were found to be real due to their daily occurrence in the industry. Their severity, occurrence, detection and coverage were classified as moderate and high, high, low and moderate, and moderate and high, respectively. The application of the CP and FMEA tools was efficient in identifying and evaluating the environmental impacts caused by the slaughter and processing of pork carcasses. Liquid slaughter effluents and solid wastes (blood and bones are the factors that pose the greatest risks to the environment. The substitution of treatment plant chemicals with decomposing microorganisms, composting, and the production of animal meal and feed from solid waste are appropriate measures the industry could adopt to minimize the contamination of water resources and soil.

  6. TRAC-PF1 choked-flow model

    International Nuclear Information System (INIS)

    Sahota, M.S.; Lime, J.F.

    1983-01-01

    The two-phase, two-component choked-flow model implemented in the latest version of the Transient Reactor analysis Code (TRAC-PF1) was developed from first principles using the characteristic analysis approach. The subcooled choked-flow model in TRAC-PF1 is a modified form of the Burnell model. This paper discusses these choked-flow models and their implementation in TRAC-PF1. comparisons using the TRAC-PF1 choked-flow models are made with the Burnell model for subcooled flow and with the homogeneous-equilibrium model (HEM) for two-phae flow. These comparisons agree well under homogeneous conditions. Generally good agreements have been obtained between the TRAC-PF1 results from models using the choking criteria and those using a fine mesh (natural choking). Code-data comparisons between the separate-effects tests of the Marviken facility and the Edwards' blowdown experiment also are favorable. 10 figures

  7. TRAC-PF1 code assessment using OECD LOFT LP-FP-1 experiment

    International Nuclear Information System (INIS)

    Barbero, F.J.

    1992-04-01

    This report assesses thermal-hydraulic aspects of LOFT LP-FP-1 experiment making use of TRAC-PF1/MOD1. LP-FP-1 experiment studies the system thermal-hydraulic and core thermal response for initial and boundary conditions similar to a large-break design basis LOCA leading to fission product release from the fuel cladding gap region. It also assesses the fission product retention effectiveness of the PWR-ECCS in best estimate conditions

  8. Failure Mode and Effect Analysis (FMEA) for confectionery manufacturing in developing countries: Turkish delight production as a case study

    OpenAIRE

    Ozilgen,Sibel

    2012-01-01

    The Failure Mode and Effect Analysis (FMEA) was applied for risk assessment of confectionary manufacturing, in whichthe traditional methods and equipment were intensively used in the production. Potential failure modes and effects as well as their possible causes were identified in the process flow. Processing stages that involve intensive handling of food by workers had the highest risk priority numbers (RPN = 216 and 189), followed by chemical contamination risks in different stages of the ...

  9. Oxidative degradation of the organometallic iron(II) complex [Fe{bis[3-(pyridin-2-yl)-1H-imidazol-1-yl]methane}(MeCN)(PMe3)](PF6)2: structure of the ligand decomposition product trapped via coordination to iron(II).

    Science.gov (United States)

    Haslinger, Stefan; Pöthig, Alexander; Cokoja, Mirza; Kühn, Fritz E

    2015-12-01

    Iron is of interest as a catalyst because of its established use in the Haber-Bosch process and because of its high abundance and low toxicity. Nitrogen-heterocyclic carbenes (NHC) are important ligands in homogeneous catalysis and iron-NHC complexes have attracted increasing attention in recent years but still face problems in terms of stability under oxidative conditions. The structure of the iron(II) complex [1,1'-bis(pyridin-2-yl)-2,2-bi(1H-imidazole)-κN(3)][3,3'-bis(pyridin-2-yl-κN)-1,1'-methanediylbi(1H-imidazol-2-yl-κC(2))](trimethylphosphane-κP)iron(II) bis(hexafluoridophosphate), [Fe(C17H14N6)(C16H12N6)(C3H9P)](PF6)2, features coordination by an organic decomposition product of a tetradentate NHC ligand in an axial position. The decomposition product, a C-C-coupled biimidazole, is trapped by coordination to still-intact iron(II) complexes. Insights into the structural features of the organic decomposition products might help to improve the stability of oxidation catalysts under harsh conditions.

  10. NbTi Strands Verification for ITER PF CICC Process Qualification of CNDA

    Science.gov (United States)

    Liu, F.; Liu, H.; Liu, S.; Liu, B.; Lei, L.; Wu, Y.

    2014-05-01

    China is in charge of most of Poloidal Field (PF) conductors production for the International Thermonuclear Experimental Reactor (ITER). The execution for PF conductors shall be in three main phases. According to ITER Procurement Arrangement (PA), the Domestic Agency (DA) shall be required to verify the room and low temperature acceptance tests carried out by the strand suppliers. As the reference laboratory of Chinese DA (CNDA), the superconducting strands test laboratory of Institute of Plasma Physics, Chinese Academy of Sciences (ASIPP) was undertaking the task of strands verification for ITER conductors. The verification test includes: diameter, Nickel plating thickness, copper-to-non-copper volume ratio, twist pitch direction and length, standard critical current (IC) and resistive transition index (n), residual resistance ration (RRR), and hysteresis loss. 48 NbTi strands with 7 billets were supplied for the PF Cable-In-Conduit Conductor (CICC) process qualification. In total, 54 samples were measured. The verification level for PF CICC process qualification was 100%. The test method, facility and results of each item are described in detail in this publication.

  11. Optimizing production and imperfect preventive maintenance planning's integration in failure-prone manufacturing systems

    International Nuclear Information System (INIS)

    Aghezzaf, El-Houssaine; Khatab, Abdelhakim; Tam, Phuoc Le

    2016-01-01

    This paper investigates the issue of integrating production and maintenance planning in a failure-prone manufacturing system. It is assumed that the system's operating state is stochastically predictable, in terms of its operating age, and that it can accordingly be preventively maintained during preplanned periods. Preventive maintenance is assumed to be imperfect, that is when performed, it brings the manufacturing system to an operating state that lies between ‘as bad as old’ and ‘as good as new’. Only an overhauling of the system brings it to a ‘as good as new’ operating state again. A practical integrated production and preventive maintenance planning model, that takes into account the system's manufacturing capacity and its operational reliability state, is developed. The model is naturally formulated as a mixed-integer non-linear optimization problem, for which an extended mixed-integer linear reformulation is proposed. This reformulation, while it solves the proposed integrated planning problem to optimality, remains quite demanding in terms of computational time. A fix-and-optimize procedure, that takes advantage of some properties of the original model, is then proposed. The reformulation and the fix-and-optimize procedure are tested on some test instances adapted from those available in the literature. The results show that the proposed fix-and-optimize procedure performs quite well and opens new research direction for future improvements. - Highlights: • Integration of production planning and imperfect preventive maintenance is explored. • Imperfect maintenance is modeled using a fitting age reduction hybrid hazard rate. • A practical approximate optimization model for this integration is proposed. • The resulting naturally MINL optimization model is reformulated and solved as a MILP. • An effective fix-and-optimize procedure is proposed for large instances of this MILP.

  12. Failure Mode and Effect Analysis (FMEA for confectionery manufacturing in developing countries: Turkish delight production as a case study

    Directory of Open Access Journals (Sweden)

    Sibel Ozilgen

    2012-09-01

    Full Text Available The Failure Mode and Effect Analysis (FMEA was applied for risk assessment of confectionary manufacturing, in whichthe traditional methods and equipment were intensively used in the production. Potential failure modes and effects as well as their possible causes were identified in the process flow. Processing stages that involve intensive handling of food by workers had the highest risk priority numbers (RPN = 216 and 189, followed by chemical contamination risks in different stages of the process. The application of corrective actions substantially reduced the RPN (risk priority number values. Therefore, the implementation of FMEA (The Failure Mode and Effect Analysis model in confectionary manufacturing improved the safety and quality of the final products.

  13. KT-2 poloidal-field(PF) system design

    Energy Technology Data Exchange (ETDEWEB)

    Han, Jang Min; Hong, Bong Keun; Hwang, Chul Kyu; Song, Woo Sub; Kim, Sung Kyu; Lee, Kwang Won [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1995-12-01

    KT-2 poloidal-field (PF) system design is described. The PF coil system is designed for both up-down symmetric double-null (DN) and up-down asymmetric single-null (SN) configurations. Three sets of `design-basis` scenarios, which describe the ohmic heating (OH), the 5MW and the high bootstrap (HIBS) baseline modes, are represented. In these cases, the power and energy required from external power supplies are calculated. 8 tabs., 54 figs., 14 refs. (Author).

  14. Failure modes and effects analysis of fusion magnet systems

    International Nuclear Information System (INIS)

    Zimmermann, M.; Kazimi, M.S.; Siu, N.O.; Thome, R.J.

    1988-12-01

    A failure modes and consequence analysis of fusion magnet system is an important contributor towards enhancing the design by improving the reliability and reducing the risk associated with the operation of magnet systems. In the first part of this study, a failure mode analysis of a superconducting magnet system is performed. Building on the functional breakdown and the fault tree analysis of the Toroidal Field (TF) coils of the Next European Torus (NET), several subsystem levels are added and an overview of potential sources of failures in a magnet system is provided. The failure analysis is extended to the Poloidal Field (PF) magnet system. Furthermore, an extensive analysis of interactions within the fusion device caused by the operation of the PF magnets is presented in the form of an Interaction Matrix. A number of these interactions may have significant consequences for the TF magnet system particularly interactions triggered by electrical failures in the PF magnet system. In the second part of this study, two basic categories of electrical failures in the PF magnet system are examined: short circuits between the terminals of external PF coils, and faults with a constant voltage applied at external PF coil terminals. An electromagnetic model of the Compact Ignition Tokamak (CIT) is used to examine the mechanical load conditions for the PF and the TF coils resulting from these fault scenarios. It is found that shorts do not pose large threats to the PF coils. Also, the type of plasma disruption has little impact on the net forces on the PF and the TF coils. 39 refs., 30 figs., 12 tabs

  15. From scootes to choppers : Product protfolio change and organizational failure - Evidence from the UK motorcycle industry 1895 to 1993

    NARCIS (Netherlands)

    Wezel, FC; van Witteloostuijn, A

    This article explores the conditions under which organizational change increases the risk of organizational failure. To reach this goal, we examine the pros and cons of flexibility and inertia arguments. Empirically, we measure the survival consequences of product portfolio expansion in the British

  16. Tissue advanced glycation end products are associated with diastolic function and aerobic exercise capacity in diabetic heart failure patients

    NARCIS (Netherlands)

    Willemsen, Suzan; Hartog, Jasper W. L.; Hummel, Yoran M.; van Ruijven, Marieke H. I.; van der Horst, Iwan C. C.; van Veldhuisen, Dirk J.; Voors, Adriaan A.

    Aims Advanced glycation end products (AGEs) are increased in patients with diabetes and are associated with diastolic dysfunction through the formation of collagen crosslinks in the heart. The association among AGEs, diastolic function, and aerobic capacity in heart failure (HF) patients with and

  17. Development of prismatic sealed nickel-cadmium battery, 2000PF

    Energy Technology Data Exchange (ETDEWEB)

    Arahi,; Kazuo,; Yoshimura, Hideaki; Takeshima, Kenji; Kawamura, Chiaki

    1988-10-21

    Though, as for the sealed Ni-Cd battery, that of cylindrical form has been majorly used, that of prismatic form is heightened in needs, with the portable electronic appliances made smaller and thinner, for which needs a new type battery 2000 PF, by new production process technology and components, was developed. As compared with the past cylindrical sealed Ni-Ca batter, generally less than 100Wh/l in energy density, the new one is higher and 133Wh/l in it, with the achievement of a 17 to 34% lightening in weight. That heightening in energy density was realized by the optimized design of lamination structure, availing of the advantage not to necessitate the electrode to be wound in a spiral form, as necessary in the cylindrical battery. While as the sealing by caulking can not be adopted like the cylindrical battery, a precise welding technique by laser was established. The assembly line is of an almost unmanned on-line computer control system. The charge and discharge characteristics, etc. were indicated in detail. 11 figures, 1 table.

  18. EPICOR-II resin degradation results from first resin samples of PF-8 and PF-20

    International Nuclear Information System (INIS)

    McConnell, J.W. Jr.; Sanders, R.D. Sr.

    1985-12-01

    The 28 March 1979 accident at Three Mile Island Unit 2 released approximately 560,000 gallons of contaminated water to the Auxiliary and Fuel Handling Buildings. The water was decontaminated using a demineralization system called EPICOR-II developed by Epicor, Inc. The Low-Level Waste Data Base Development - EPICOR-II Resin/Liner Investigation Project is studying the chemical and physical conditions of the synthetic ion exchange resins found in several EPICOR-II prefilters. This report summarizes results and analyses of the first sampling of ion exchange resins from EPICOR-II prefilters PE-8 and -20. Results are compared with baseline data from tests performed on unirradiated Epicor, Inc. resins to determine if degradation has occurred due to the high internal radiation dose received by the EPICOR-II resins. Results also are compared with recent findings on resin degradation by Battelle Columbus Laboratories and Brookhaven National Laboratory. Analyses comparing test results of resins from EPICOR-II prefilters PF-8 and -20 with unirradiated resins obtained from Epicor, Inc. show resin degradation has occurred in some of the EPICOR-II resins examined. The mechanism of degradation is compared with work of other researchers and is consistent with their findings. The strong acid cation resins (divinylbenzene, styrene base structure) are losing effective cross-linking along with scission of functional groups and are experiencing first an increase and eventually a decrease in total exchange capacity as the absorbed radiation dose increases. The phenolic cation resins (phenol-formaldehyde base structure) show a loss of effective cross-linking and oxidation of the polymer chain. Analyses of resins removed from EPICOR-II prefilters PF-8 and -20 over the next several years should show a further increase in degradation

  19. Capillary Electrophoresis as Analysis Technique for Battery Electrolytes: (i Monitoring Stability of Anions in Ionic Liquids and (ii Determination of Organophosphate-Based Decomposition Products in LiPF6-Based Lithium Ion Battery Electrolytes

    Directory of Open Access Journals (Sweden)

    Marcelina Pyschik

    2017-09-01

    Full Text Available In this work, a method for capillary electrophoresis (CE hyphenated to a high-resolution mass spectrometer was presented for monitoring the stability of anions in ionic liquids (ILs and in commonly used lithium ion battery (LIB electrolytes. The investigated ILs were 1-methyl-1-propylpyrrolidinium bis(trifluoromethanesulfonylimide (PYR13TFSI and 1-methyl-1-propylpyrrolidinium bis(fluorosulfonylimide (PYR13FSI. The method development was conducted by adjusting the following parameters: buffer compositions, buffer concentrations, and the pH value. Also the temperature and the voltage applied on the capillary were optimized. The ILs were aged at room temperature and at 60 °C for 16 months each. At both temperatures, no anionic decomposition products of the FSI− and TFSI− anions were detected. Accordingly, the FSI− and TFSI− anions were thermally stable at these conditions. This method was also applied for the investigation of LIB electrolyte samples, which were aged at 60 °C for one month. The LP30 (50/50 wt. % dimethyl carbonate/ethylene carbonate and 1 M lithium hexafluorophosphate electrolyte was mixed with the additive 1,3-propane sultone (PS and with one of the following organophosphates (OP: dimethyl phosphate (DMP, diethyl phosphate (DEP, and triethyl phosphate (TEP, to investigate the influence of these compounds on the formation of OPs.

  20. Electrochemical studies of UO22+ in 1-octyl 3- methylimidazolium hexafluorophosphate (omimPF6) room temperature ionic liquid

    International Nuclear Information System (INIS)

    Kamat, J.V.; Gopinath, N.; Lohithakshan, K.V.; Aggarwal, S.K.

    2007-01-01

    The effect of the degraded products on the Electrochemical Window (EW) of l-octyl 3- methylimidazolium hexafluorophosphate (OmimPF 6 ) were studied. Studies were also carried to optimize conditions for extraction of UO 2 2+ to investigate its redox behavior in OmimPF6 and determine the number of electrons involved in the redox chemistry. The Chronoamperometric technique of evaluating n without knowing diffusion coefficient, D, was devised by Kakihana et al. and has been applied in the present work. The number of electrons involved was determined from the values of slope and intercept of the Cottrell plot. The results of these studies are presented in this paper

  1. Prescription pattern of Chinese herbal products for heart failure in Taiwan: A population-based study.

    Science.gov (United States)

    Tsai, Ming-Yen; Hu, Wen-Long; Lin, Che-Chen; Lee, Yi-Chiao; Chen, Shih-Yu; Hung, Yu-Chiang; Chen, Yung-Hsiang

    2017-02-01

    Certain Chinese herbal products (CHPs) may protect against the progression of heart failure (HF). However, there is a lack of research regarding the use of CHPs in patients with HF. The aims of this study were to analyze CHPs usage patterns in patients with HF and to identify the frequency and combination of CHPs most commonly used for HF. This retrospective, nationwide, population-based cohort study was conducted using a randomly sampled cohort of one million patients selected from the National Health Insurance Research Database (NHIRD) for the years 2000-2010 in Taiwan. CHP use and the top ten most frequently prescribed formulae and single herbs for treating HF were assessed, including total formulae number and average and frequency of prescriptions. Demographic characteristics, including sex and age at diagnosis of HF, were examined, together with existing comorbidities. The cohort included 19,988 newly diagnosed AD patients, who were given CHP treatment for HF between 2000 and 2010. Among them, female patients (53.3%) and those over 65years old (63.9%) were more likely to use CM. After adjusting for demographic factors, HF patients suffering from coronary artery disease (CAD) were more likely to seek traditional Chinese medicine (TCM) treatment than those with non-TCM users (57.6% vs. 52.6%). Zhi-Gan-Cao-Tang (4.07%) and Danshen (5.13%) were the most frequent formula CHP and single CHP prescribed by TCM practitioners for treating HF, respectively. Most people with HF who consumed CHPs used CHPs to supplement Yang-Qi, nourish the Ying-blood, and strengthen the heart spirit as complementary medicines to relieve HF-related symptoms, in addition to using standard anti-HF treatments. Further large-scale, randomized clinical trials are warranted in order to determine the effectiveness and safety of these herbal medicines. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  2. 21PF overpacks: Phenolic-foam induced corrosion

    International Nuclear Information System (INIS)

    Kovac, F.M.

    1994-01-01

    The 21PF overpack was developed in the 1960s and approved for use in the 1970s by the US Department of Transportation (DOT). This package, used for the transport of uranium hexafluoride enriched >1%, has had a history of severe metal corrosion, water ingress, and subsequent leakage. Problems associated with corrosion and water leaking from 21PF overpacks caused the DOT to seek public comments and to undertake rulemaking action. As a result, the DOT required modifications and refurbishment of existing overpacks, and specification changes for the fabrication of new 21PF overpacks. Recent studies conducted by the roofing industry indicate that phenolic foam has caused severe corrosion in metal roofing structures, and its use is being curtailed. These findings need to be explored in order to determine if phenolic foam in 21PF overpacks causes corrosion and compromises the package integrity. Metallic corrosion induced by phenolic foam may affect the continued use of the 21PF overpack because damage to the structural integrity of the metal parts of the packaging will affect its ability to meet design specifications

  3. The Structure of Plasmodium falciparum Blood-Stage 6-Cys Protein Pf41 Reveals an Unexpected Intra-Domain Insertion Required for Pf12 Coordination.

    Directory of Open Access Journals (Sweden)

    Michelle L Parker

    Full Text Available Plasmodium falciparum is an apicomplexan parasite and the etiological agent of severe human malaria. The complex P. falciparum life cycle is supported by a diverse repertoire of surface proteins including the family of 6-Cys s48/45 antigens. Of these, Pf41 is localized to the surface of the blood-stage merozoite through its interaction with the glycophosphatidylinositol-anchored Pf12. Our recent structural characterization of Pf12 revealed two juxtaposed 6-Cys domains (D1 and D2. Pf41, however, contains an additional segment of 120 residues predicted to form a large spacer separating its two 6-Cys domains. To gain insight into the assembly mechanism and overall architecture of the Pf12-Pf41 complex, we first determined the 2.45 Å resolution crystal structure of Pf41 using zinc single-wavelength anomalous dispersion. Structural analysis revealed an unexpected domain organization where the Pf41 6-Cys domains are, in fact, intimately associated and the additional residues instead map predominately to an inserted domain-like region (ID located between two β-strands in D1. Notably, the ID is largely proteolyzed in the final structure suggesting inherent flexibility. To assess the contribution of the ID to complex formation, we engineered a form of Pf41 where the ID was replaced by a short glycine-serine linker and showed by isothermal titration calorimetry that binding to Pf12 was abrogated. Finally, protease protection assays showed that the proteolytic susceptibility of the ID was significantly reduced in the complex, consistent with the Pf41 ID directly engaging Pf12. Collectively, these data establish the architectural organization of Pf41 and define an essential role for the Pf41 ID in promoting assembly of the Pf12-Pf41 heterodimeric complex.

  4. Mathematical modeling of a multi-product EMQ model with an enhanced end items issuing policy and failures in rework.

    Science.gov (United States)

    Chiu, Yuan-Shyi Peter; Sung, Peng-Cheng; Chiu, Singa Wang; Chou, Chung-Li

    2015-01-01

    This study uses mathematical modeling to examine a multi-product economic manufacturing quantity (EMQ) model with an enhanced end items issuing policy and rework failures. We assume that a multi-product EMQ model randomly generates nonconforming items. All of the defective are reworked, but a certain portion fails and becomes scraps. When rework process ends and the entire lot of each product is quality assured, a cost reduction n + 1 end items issuing policy is used to transport finished items of each product. As a result, a closed-form optimal production cycle time is obtained. A numerical example demonstrates the practical usage of our result and confirms a significant savings in stock holding and overall production costs as compared to that of a prior work (Chiu et al. in J Sci Ind Res India, 72:435-440 2013) in the literature.

  5. Drugs and personality: comparison of drug users, nonusers, and other clinical groups on the 16PF.

    Science.gov (United States)

    Spotts, J V; Shontz, F C

    1991-10-01

    This article reviews published 16PF research on drug users. It also compares the 16PF scores of a new sample of nonusers with scores of matched groups of heavy, chronic users of cocaine, amphetamine, opiates, and barbiturates/sedative hypnotics, as well as combined groups of stimulant users, depressant users, and a combined group of users of all substances. No significant differences were found among drug user groups, but the profile of the nonuser group was distinctive. K-Means Cluster Analyses, as well as Cattell's Similarity and Pearson Product Moment Correlation Coefficients, were used to compare profiles of these new samples with the 19 groups described in an earlier meta-analysis of published 16PF studies. Data from the new samples did not cluster with data from other published research, although certain specific similarities appeared in more detailed correlational analyses. Methodological problems are discussed, and it is recommended that in future studies drug user groups be more carefully selected and defined, sample descriptions be more thorough and complete, complete profile information be routinely provided, and efforts be made to explore the utility of the Cattell CAQ in studies of drug users/misusers.

  6. Diagnostics of PF-1000 facility operation and plasma concentration on the basis of spectral measurements

    International Nuclear Information System (INIS)

    Skladnik-Sadowska, E.; Malinowski, K.; Sadowski, M.J.; Scholz, M.; Tsarenko, A.V.

    2005-01-01

    The paper concerns the monitoring of the operation of high-current pulse discharges and the determination of the plasma concentration within the dense magnetized plasma column by means of optical spectroscopy methods. In experiments performed within the large PF-1000 facility, which is operated at IPPLM in Warsaw, particular attention was paid to possibility of the determination of correctness of the operational mode. In order to measure the visible radiation (VR), as emitted from the collapsing current sheath and the dense pinch region, the use was made of the MECHELLE R 900-optical-spectrometer, which was equipped with a CCD measuring head. The spectral measurements were performed at an angle of about 650 to the symmetry axis of the PF electrode system, through an optical window and a special collimator coupled with the quartz optical-cable. The observed VR emission originated from a part of the inner- and outer-electrode surfaces, the collapsing current-sheath layer and a portion of the dense plasma pinch-region (located a distance of 40-50 mm from the electrode ends). Considerable differences were found in the optical spectra recorded for so-called good shots and for cases of some failures. In the case of a breakdown (damage) of the main insulator there were observed different Al-lines, which originated from the eroded insulator material. At so-called bad vacuum conditions there were recorded various C-lines, and at an uncontrolled air-leakage into the experimental chamber there appeared numerous N-lines. The appearance of these characteristic spectral lines made possible to determine whether the operation of the PF-1000 facility was correct or incorrect. The paper reports also on estimates of plasma concentration values, which have been performed on the basis of a quantitative analysis of the Stark broadening of the selected spectral lines. (author)

  7. Relationship between advanced glycation end-products with the severity of chronic heart failure in 85 patients

    Directory of Open Access Journals (Sweden)

    Amir Farhang Zand Parsa

    2013-12-01

    Full Text Available Background: Advanced glycation end-products (AGEs came up with the recent researches regarding new biomarkers for the diagnosis of heart failure. AGEs are the end products of non-enzymatic glycation and oxidation of proteins, lipids and nucleotides during Maillard biochemical reaction. Although it has been known that AGEs have a role in the pathogenesis of chronic heart failure (CHF, information regarding its role and its pathogenetic mechanism is very limited. The aim of this study was to find any relationship between AGEs with the etiology and severity of chronic heart failure.Methods: This study is a prospective cross sectional study that enrolled 85 patients with chronic heart failure. Measurement of left ventricle ejection fraction (LVEF was done by echocardiography. Blood samples were collected for measuring AGEs just before or after echocardiography assessment (in the same session. Measurement of AGEs was done by the enzyme-linked immunosorbent assay (ELISA method. The relationship between AGEs with the severity of CHF and as well as the etiology of CHF were evaluated via SPSS-15.Results: Of 85 patients 48 (56.5% patients were male and 37 (43.5% were female; Mean±SD of their ages was 55.8±13.4 years old (ranges from 27 to 84 years. Correlation coefficient between LVEF and AGEs was 0.269 (P=0.013. Mean of AGEs in patients with and without ischemic etiology of their heart failure were 16.8±9.8µg/ml and 11.6±7.3 µg/ml, respectively. Although trend was in favor of ischemic heart failure, the difference between two groups was not statistically significant (P= 0.141.Conclusion: According to this study the rate of AGES could be helpful in the diagnosis and assessment of severity of CHF. Based on our findings, higher blood levels of AGEs in the ischemic CHF cases, also it could be concluded that in the future this marker may be used for etiologic differentiation of heart failure syndrome.

  8. Identification of Immunodominant Responses to the Plasmodium falciparum Antigens PfUIS3, PfLSA1 and PfLSAP2 in Multiple Strains of Mice.

    Directory of Open Access Journals (Sweden)

    Rhea J Longley

    Full Text Available Malaria, caused by the Plasmodium parasite, remains a serious global public health concern. A vaccine could have a substantial impact on eliminating this disease, alongside other preventative measures. We recently described the development of three novel, viral vectored vaccines expressing either of the antigens PfUIS3, PfLSA1 and PfLSAP2. Each vaccination regimen provided high levels of protection against chimeric parasite challenge in a mouse model, largely dependent on CD8+ T cells. In this study we aimed to further characterize the induced cellular immune response to these vaccines. We utilized both the IFNγ enzyme-linked immunosorbent spot assay and intracellular cytokine staining to achieve this aim. We identified immunodominant peptide responses for CD4+ and CD8+ T cells for each of the antigens in BALB/c, C57BL/6 and HLA-A2 transgenic mice, creating a useful tool for researchers for subsequent study of these antigens. We also compared these immunodominant peptides with those generated from epitope prediction software, and found that only a small proportion of the large number of epitopes predicted by the software were identifiable experimentally. Furthermore, we characterized the polyfunctionality of the induced CD8+ T cell responses. These findings contribute to our understanding of the immunological mechanisms underlying these protective vaccines, and provide a useful basis for the assessment of these and related vaccines as clinical constructs.

  9. Integrated load distribution and production planning in series-parallel multi-state systems with failure rate depending on load

    International Nuclear Information System (INIS)

    Nourelfath, Mustapha; Yalaoui, Farouk

    2012-01-01

    A production system containing a set of machines (also called components) arranged according to a series-parallel configuration is addressed. A set of products must be produced in lots on this production system during a specified finite planning horizon. This paper presents a method for integrating load distribution decisions, and tactical production planning considering the costs of capacity change and the costs of unused capacity. The objective is to minimize the sum of capacity change costs, unused capacity costs, setup costs, holding costs, backorder costs, and production costs. The main constraints consist in satisfying the demand for all products over the entire horizon, and in not exceeding available repair resource. The production series-parallel system is modeled as a multi-state system with binary-state components. The proposed model takes into account the dependence of machines' failure rates on their load. Universal generating function technique can be used in the optimization algorithm for evaluating the expected system production rate in each period. We show how the formulated problem can be solved by comparing the results of several multi-product lot-sizing problems with capacity associated costs. The importance of integrating load distribution decisions and production planning is illustrated through numerical examples.

  10. Motivational Distortion on 16 PF Primaries by Male Felons.

    Science.gov (United States)

    Wallbrown, Fred H.; And Others

    1989-01-01

    Investigated motivational distortion using the 16 Personality Factor Questionnaire (16 PF) for male (n=331) criminal offenders. Results indicated that ego-strength, free-floating anxiety, ability to bind anxiety, boldness, dominance, emotional sensitivity and suspiciousness contributed most to the motivational distortion scale predictions.…

  11. Trypanosoma cruzi: avirulence of the PF strain to Callithrix marmosets

    Directory of Open Access Journals (Sweden)

    Humberto Menezes

    1981-06-01

    Full Text Available Callithrix jacchus geoffroy marmosets (HumBol. 1812 were injected once subcutaneously with 10.000 parasites/g body weight and followed for a period of six months. The PF strain of Trypanosoma cruzi was used. Follow-up was done through blood cultures, xenodiagnosis, serological tests, and ECG. A small number of normaI animais served as control.

  12. Properties of Sago Particleboards Resinated with UF and PF Resin

    Directory of Open Access Journals (Sweden)

    Chen Chiang Tay

    2016-01-01

    Full Text Available The sago processing industry in Mukah, Sarawak, had generated huge amount of sago waste after the milling process and scientists have employed the waste into composite material. In this work, sago residues were mixed with the Phenol Formaldehyde (PF and Urea Formaldehyde (UF for particleboard fabrication. The fabrication and testing methods are based on JIS A 5908 Standard. A single layer particleboard using sago particles was fabricated at targeted density of 600 kg/m3. Particles with weight fractions of 90%, 85%, and 80% with two different matrices were used in the fabrication. The results demonstrated that the samples with different weight fraction and matrix have great influence on the mechanical properties such as MOR, MOE, Young’s Modulus, tensile strength, impact strength, screw test, and internal bonding. The sago UF/PF particleboard only displays single stage decomposition. All the panels underwent physical tests which are water absorption and thickness swelling. The combination of sago particles with UF/PF can be utilized for general indoor application purposes such as furniture manufacturing. Sago particleboard made by UF/PF provided the advantages like optimized performance, minimized weight and volume, cost effectiveness, chemical resistance, and resistance to biodegradation.

  13. Seismic analysis of ITER fourth PF (Poloidal Field Coil) feeder

    International Nuclear Information System (INIS)

    Liu, Sumei; Chen, Wei; Song, Yuntao; Ni, Xiaojun; Wang, Zhongwei; Chen, Yonghua; Gong, Chenyu

    2014-01-01

    The ITER feeder systems connect the ITER magnet systems located inside the main cryostat to the cryo-plant, power-supply and control system interfaces outside the cryostat. The main purpose of the feeders is to convey the cryogenic supply and electrical power to the coils as well as house the instrumentation wiring. The PF busbar which carries 52 kA current will suffer from high Lorentz force due to the background magnetic field inspired by the coils and the self-field between every pair of busbars. Except their mechanical strength and thermal insulation performance must be achieved, the dynamic mechanism on PF structure should be assessed. This paper presents the simulation and seismic analysis on ITER 4th PF feeder including the Coil Terminal Box and S-bend Box (CTB and SBB), the Cryostat Feed-through (CFT), the In-Cryostat-Feeder (ICF), especially for the ground supports and main outer-tube firstly. This analysis aims to study seismic resistance on system design under local seismograms with floor response spectrum, the structural response vibration mode and response duration results of displacement, membrane stress, and bending stress on structure under different directions actuating signals were obtained by using the single-seismic spectrum analysis and Dead Weight analysis respectively. Based on the simulative and analytical results, the system seismic resistance and the integrity of the support structure in the 4th PF feeder have been studied and the detail design confirmed

  14. Nature and climatology of Pfänderwind

    Directory of Open Access Journals (Sweden)

    Alexander Gohm

    2015-04-01

    Full Text Available The characteristics and climatology of Pfänderwind, a largely unknown downslope windstorm near the town of Bregenz (Austria at the entrance of the Rhine Valley, are investigated based on an eleven-year dataset of weather station observations and ERA-Interim reanalyses. The goal is to clarify the inconsistency in the definition of this phenomenon, to illuminate its dynamics, and to quantify its frequency of occurrence. It is shown that Pfänderwind has similarities to foehn but does occur for different synoptic-scale conditions. Moreover, two types of Pfänderwind have to be distinguished: Type 1, or classical Pfänderwind, is associated with easterly to northeasterly large-scale flow that crosses the Pfänder mountain range, descends in a foehn-like manner and causes moderate to strong winds in the town of Bregenz and its vicinity. The temperature anomaly induced at the surface by adiabatic warming is small as a result of weak low-level stability. Type-1 events occur on average 12 times per year, preferentially in spring, and most frequently between the afternoon and midnight. Type 2, or southeast Pfänderwind, is associated with westerly to southwesterly ambient winds near the main Alpine crest level. The Rhine valley is filled with cold air and in most cases south foehn is not present. However, the synoptic and meso-scale pressure gradient favours southerly ageostrophic flow in the Rhine Valley especially near the top of the cold-air pool. This flow passes the Gebhardsberg, the southwestern extension of the Pfänder mountain range, descends on its leeward side and causes strong foehn-like warming at the surface. However, southerly to southeasterly near-surface winds at Bregenz are rather weak. Type-2 events occur on average 40 times per year, most frequently between the evening and the early morning, and exhibit a weak seasonal dependence. More than half of all type-1 and type-2 events last only one or two hours.

  15. Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax

    Science.gov (United States)

    Costa, Joana D'Arc Neves; Zanchi, Fernando Berton; Rodrigues, Francisco Lurdevanhe da Silva; Honda, Eduardo Rezende; Katsuragawa, Tony Hiroschi; Pereira, Dhélio Batista; Taborda, Roger Lafontaine Mesquita; Tada, Mauro Shugiro; Ferreira, Ricardo de Godoi Mattos; Pereira-da-Silva, Luiz Hildebrando

    2013-01-01

    The PfCLAG9 has been extensively studied because their immunogenicity. Thereby, the gene product is important for therapeutics interventions and a potential vaccine candidate. Antibodies against synthetic peptides corresponding to selected sequences of the Plasmodium falciparum antigen PfCLAG9 were found in sera of falciparum malaria patients from Rondônia, in the Brazilian Amazon. Much higher antibody titres were found in semi-immune and immune asymptomatic parasite carriers than in subjects suffering clinical infections, corroborating original findings in Papua Guinea. However, sera of Plasmodium vivax patients from the same Amazon area, in particular from asymptomatic vivax parasite carriers, reacted strongly with the same peptides. Bioinformatic analyses revealed regions of similarity between P. falciparum Pfclag9 and the P. vivax ortholog Pvclag7. Indirect fluorescent microscopy analysis showed that antibodies against PfCLAG9 peptides elicited in BALB/c mice react with human red blood cells (RBCs) infected with both P. falciparum and P. vivax parasites. The patterns of reactivity on the surface of the parasitised RBCs are very similar. The present observations support previous findings that PfCLAG9 may be a target of protective immune responses and raises the possibility that the cross reactive antibodies to PvCLAG7 in mixed infections play a role in regulate the fate of Plasmodium mixed infections. PMID:23440122

  16. A nuclear magnetic resonance study of (TMTSF) 2PF 6

    Science.gov (United States)

    McBrierty, V. J.; Douglass, D. C.; Wudl, F.

    1982-09-01

    Inverse linewidths and spin-lattice relaxation times of fluorine and proton magnetic resonance spectra are used to examine molecular motion in the organic superconductor (TMTSF) 2PF 6. The results clearly show that rotation of the PF 6- anion is the principal agent for the observed relaxation of fluorine contrary to some suggestions in the current literature. This interpretation is based upon qualitative comparison with relaxation in plastic crystals, where molecular rotation is well characterized, and upon the quantitative agreement between the calculated and observed linewidth change near 90K and the maximum spin-lattice relaxation rate at 140K. There is also motional evidence, supported by X-ray structure measurements, that a phase transition occurs in the vicinity of 160K.

  17. PWR plant transient analyses using TRAC-PF1

    International Nuclear Information System (INIS)

    Ireland, J.R.; Boyack, B.E.

    1984-01-01

    This paper describes some of the pressurized water reactor (PWR) transient analyses performed at Los Alamos for the US Nuclear Regulatory Commission using the Transient Reactor Analysis Code (TRAC-PF1). Many of the transient analyses performed directly address current PWR safety issues. Included in this paper are examples of two safety issues addressed by TRAC-PF1. These examples are pressurized thermal shock (PTS) and feed-and-bleed cooling for Oconee-1. The calculations performed were plant specific in that details of both the primary and secondary sides were modeled in addition to models of the plant integrated control systems. The results of these analyses show that for these two transients, the reactor cores remained covered and cooled at all times posing no real threat to the reactor system nor to the public

  18. Introduction and immigration of TRAC-PF1 code

    International Nuclear Information System (INIS)

    Yan Yuhua; Gao Zuying; Gao Cheng; Li Jincai

    1997-01-01

    TRAC-PF1 code performs best-estimate predictions of postulated accidents for pressurized light water reactors. It is one of the few system analyse codes which use two fluid model to treat two phase problems in nuclear system. In order to use this advanced software in China and make it possible to be run in different compute systems, IBM version of TRAC-PF1 code, imported from USA National Energy Software Center, is immigrated to CDC NOS/VE system and SUN workstation. The differences in computer languages from IBM 370 to CDC NOS/VE and to SUN workstation are modified properly. All the benchmark problems are calculated, and the results show that the immigration is successful

  19. TRAC-PF1/MOD2 status and plans

    International Nuclear Information System (INIS)

    Spore, J.W.; Steinke, R.G.; Nelson, R.A.; Cappiello, M.W.; Jenks, R.

    1989-01-01

    The development of the TRAC-PF1/MOD1 code was completed in July 1988 with the release of Version 14.4. A TRAC-PF1/MOD2 code development plan addresses code deficiencies identified in the MOD1 code in order to provide an accurate and defensible tool that can be used to simulate large-break loss-of-coolant accidents (LOCAs), small-break LOCAs, and operational transients. The MOD2 code development plan is an international cooperative effort that includes contributions from Los Alamos National Laboratory, Idaho National Engineering Laboratory (INEL), Japanese Atomic Energy Research Institute (JAERI), Cray Research, Central Electricity Generating Board (CEGB), and United Kingdom Atomic Energy Authority (UKAEA)

  20. TRAC-PF1 analyses of potential pressurized-thermal-shock transients at a Combustion-Engineering PWR

    International Nuclear Information System (INIS)

    Koenig, J.E.; Spriggs, G.D.; Smith, R.C.

    1984-01-01

    Los Alamos is participating in a program to assess the risk of pressurized thermal shock (PTS) to a reactor vessel. Our role is to provide best-estimate thermal-hydraulic analyses of 12 postulated overcooling transients using TRAC-PF1. These transients are hypothetical and include multiple operator/equipment failures. Calvert Cliffs/Unit-1, a Combustion-Engineering plant, is the pressurized water reactor modeled for this study. The utility and the vendor supplied information for the comprehensive TRAC-PF1 model. Secondary and primary breaks from both hot-zero-power and full-power conditions were simulated for 7200 s (2 h). Low bulk temperatures and loop-flow stagnation while the system was at a high pressure were of particular interest for PTS analysis. Three transients produced primary temperatures below 405 K (270 0 F - the NRC screening criterion) with system repressurization. Six transients indicated flow stagnation would occur in one loop but not both. One transient showed flow stagnation might occur in both loops. Oak Ridge National Laboratory will do fracture-mechanics analysis using these TRAC-PF1 results and make the final determination of the risk of PTS

  1. Medtner. Piano concertos. Geoffrey Tozer (pf) / Bryce Morrison

    Index Scriptorium Estoniae

    Morrison, Bryce

    1992-01-01

    Uuest heliplaadist "Medtner. Piano concertos. Geoffrey Tozer (pf) London Philarmonic Orchestra, Neeme Järvi. Chandos CHAN 9038/9 (two discs, oas 73 and 54 minutes). Also available as a two-disc set CD CHAN 9040. CHAN 9038: Piano concertos - N2 Op. 50; N 3 Op. 60 "Ballade" CHAN 9039 Piano concerto N 1 in C minor. Sonata-Ballade in Fsharp major Op. 27"

  2. Ion beams from high-current PF facilities

    Energy Technology Data Exchange (ETDEWEB)

    Sadowski, M [Soltan Inst. for Nuclear Studies, Otwock-Swierk (Poland)

    1997-12-31

    Pulsed beams of fast deuterons and impurity or admixture ions emitted from high-current PF-type facilities operated in different laboratories are dealt with. A short comparative analysis of time-integrated and time-resolved studies is presented. Particular attention is paid to the microstructure of such ion beams, and to the verification of some theoretical models. (author). 5 figs., 19 refs.

  3. Combining information flow and physics-of-failure in mechatronic products

    NARCIS (Netherlands)

    Magniez, C.A.A.

    2007-01-01

    A continuous acceleration in the rate of technological development, shorter product life cycles, more intense competition due to maturing of markets and globalization, have forced firms to increasingly rely on new products for sales and profitability. However, reliability and quality management

  4. Analysis of user-centered failure mechanisms in new product development for quality improvement

    NARCIS (Netherlands)

    Koca, A.; Schouwenaar, A.J.M.; Brombacher, A.C.

    2007-01-01

    In the past, quality and reliability measures of products were mainly for technical component performances. However, today's global market conditions led to the realization that the dominating indicator of product qualityand reliability is often customer satisfaction. This is justified by the

  5. A Novel RSPF Approach to Prediction of High-Risk, Low-Probability Failure Events

    Data.gov (United States)

    National Aeronautics and Space Administration — Particle filters (PF) have been established as the de facto state of the art in failure prognosis, and particularly in the representation and management of...

  6. The success and failure of product development in the Danish food sector

    DEFF Research Database (Denmark)

    Kristensen, Kai; Østergaard, Peder; Juhl, Hans Jørn

    1997-01-01

    development should include factors such as: market share, sales objectives, profit objectives, technical aspects, impact on the company and company reputation and timing of the product development process. The main aim of the survey is to analyse the relationship between success in product development....... Learning is assumed to have a positive impact on the quality of prototypes being developed by the individual company, and the mathematical derivations in this paper show that there reason to believe that there is a linear relationship between the launching rate and the success rate. 5. The empirical...... in anticipating future changes and need for new products. A key factor in quality is customer information and ability to use such information. 2. Defining and doing quantitative survey of success in product development is a difficult task. It is generally agreed that the measurement of success in product...

  7. TRAC-PF1/MOD1 computer code

    International Nuclear Information System (INIS)

    Liles, D.R.; Mahaffy, J.H.

    1983-01-01

    TRAC-PF1 was designed to improve the ability of TRAC-PD2 to handle small-break LOCAs and other transients. TRAC-PF1 has all of the major improvements of TRAC-PD2 but, in addition, uses a full two-fluid model with two-step numerics in the one-dimensional components. The two-fluid model, in conjunction with a stratified-flow regime, handles countercurrent flow better than the drift-flux model previously used. The two-step numerics allow large time steps to be taken for slow transients. TRAC-PF1/MOD1 was designed to provide full balance-of-plant modeling capabilities. This required addition of a general capability for modeling plant control systems. The steam generator model was replaced to allow a wider variety of feedwater connections and better modeling of steam tube ruptures. A special turbine component also has been added, but new components were not required for adequate modeling of condensors, heaters, and pumps in the secondary system

  8. TRAC-PF1/MOD1 assessment at Los Alamos

    International Nuclear Information System (INIS)

    Knight, T.D.

    1984-01-01

    The Los Alamos National Laboratory is developing the Transient Reactor Analysis Code (TRAC) to provide an advanced best-estimate predictive capability for the analysis of postulated accidents in pressurized water reactors (PWRs). Over the past several years, four distinct versions of the code have been released; each new version introduced improvements to the existing models and numerics and added new models to extend the applications of the code. The first goal of the code was to analyze large-break loss-of-coolant accidents (LOCAs), and the TRAC-P1A and TRAC-PD2 codes primarily addressed the large-break LOCA. (The TRAC-PD2/MOD1 code is essentially the same as the TRAC-PD2 code but it also includes a released set of error corrections.) The TRAC-PF1 code contained major changes to the models and trips and to the numerical methods. These modifications enhanced the computational speed of the code and improved the application to small-break LOCAs. The TRAC-PF1/MOD1 code, the latest released version, added improved steam-generator modeling, a turbine component, and a control system together with modified constitutive relations to model the balance of plant on the secondary side and to extend the applications to non-LOCA transients. The TRAC-PF1/MOD1 code also contains reasonably general reactor-kinetics modeling to facilitate the simulation of transients with delayed scram or without scram. 13 references, 24 figures

  9. Production Planning of a Failure-Prone Manufacturing System under Different Setup Scenarios

    Directory of Open Access Journals (Sweden)

    Guy-Richard Kibouka

    2016-01-01

    Full Text Available This paper presents a control problem for the optimization of the production and setup activities of an industrial system operating in an uncertain environment. This system is subject to random disturbances (breakdowns and repairs. These disturbances can engender stock shortages. The considered industrial system represents a well-known production context in industry and consists of a machine producing two types of products. In order to switch production from one product type to another, a time factor and a reconfiguration cost for the machine are associated with the setup activities. The parts production rates and the setup strategies are the decision variables which influence the inventory and the capacity of the system. The objective of the study is to find the production and setup policies which minimize the setup and inventory costs, as well as those associated with shortages. A modeling approach based on stochastic optimal control theory and a numerical algorithm used to solve the obtained optimality conditions are presented. The contribution of the paper, for industrial systems not studied in the literature, is illustrated through a numerical example and a comparative study.

  10. Rational screening of peroxisome proliferator-activated receptor-γ agonists from natural products: potential therapeutics for heart failure.

    Science.gov (United States)

    Chen, Rui; Wan, Jing; Song, Jing; Qian, Yan; Liu, Yong; Gu, Shuiming

    2017-12-01

    Peroxisome proliferator-activated receptor-γ (PPARγ) is a member of the nuclear hormone receptor superfamily of ligand-activated transcription factors. Activation of PPARγ pathway has been shown to enhance fatty acid oxidation, improve endothelial cell function, and decrease myocardial fibrosis in heart failure. Thus, the protein has been raised as an attractive target for heart failure therapy. This work attempted to discover new and potent PPARγ agonists from natural products using a synthetic strategy of computer virtual screening and transactivation reporter assay. A large library of structurally diverse, drug-like natural products was compiled, from which those with unsatisfactory pharmacokinetic profile and/or structurally redundant compounds were excluded. The binding mode of remaining candidates to PPARγ ligand-binding domain (LBD) was computationally modelled using molecular docking and their relative binding potency was ranked by an empirical scoring scheme. Consequently, eight commercially available hits with top scores were selected and their biological activity was determined using a cell-based reporter-gene assay. Four natural product compounds, namely ZINC13408172, ZINC4292805, ZINC44179 and ZINC901461, were identified to have high or moderate agonistic potency against human PPARγ with EC 50 values of 0.084, 2.1, 0.35 and 5.6 μM, respectively, which are comparable to or even better than that of the approved PPARγ full agonists pioglitazone (EC 50  =   0.16 μM) and rosiglitazone (EC 50  =   0.034 μM). Hydrophobic interactions and van der Waals contacts are the primary chemical forces to stabilize the complex architecture of PPARγ LBD domain with these agonist ligands, while few hydrogen bonds, salt bridges and/or π-π stacking at the complex interfaces confer selectivity and specificity for the domain-agonist recognition. The integrated in vitro-in silico screening strategy can be successfully applied to rational discovery of

  11. Deuteration of acetanilides and other substituted aromatics using [Ir(COD)(Cy3P)(Py)]PF6 as catalyst

    International Nuclear Information System (INIS)

    Hesk, D.; Das, P.R.; Evans, B.

    1995-01-01

    Deuterium exchange labelling using [Ir(COD)(Cy 3 P)(Py)]PF 6 as catalyst and deuterium gas was studied on a number of substituted acetanilides. In most cases products containing deuterium ortho to the anilide group were obtained with a high degree of enrichment. With one exception no evidence for meta, para or anilide methyl labelling was seen. The catalyst was also effective in the ortho deuteration of acetophenone, benzophenone and the β-lactam containing compound Sch 48461. (Author)

  12. ANALISIS TOTAL PRODUCTIVE MAINTENANCE DENGAN METODE OVERALL EQUIPMENT EFFECTIVENESS DAN FUZZY FAILURE MODE AND EFFECTS ANALYSIS

    Directory of Open Access Journals (Sweden)

    Supriyadi Supriyadi

    2017-11-01

    Full Text Available Ash Handling System merupakan suatu bagian dari pembangkit listrik tenaga uap dengan bahan bakar batu bara yang berfungsi untuk menyalurkan limbah pembuangan sisa hasil proses pembakaran batu bara pada boiler. Sisa pembakaran terbagi menjadi fly ash dan bottom ash. Untuk sisa pembakaran fly ash akan disalurkan menuju ke Electrostatic Precipitator untuk ditangkap  dengan metode corona dan ditransfer menuju penampungan fly ash dengan cara dimampatkan bersama udara dari kompresor yang melalui pipa-pipa dan tabung transporter. Sedangkan untuk sisa pembakaran bottom ash akan disalurkan dengan alat yang disebut SSC (Submerged Scraper Conveyor. Gangguan pada SSC dapat terjadi mulai dari belt putus, masalah pada penggerak, hingga masalah pada kelistrikan dan instrumennya. Penelitian ini bertujuan untuk mengetahui nilai OEE, mengetahui dampak gangguan belt sobek, mengetahui penyebab terjadinya belt conveyor sobek dan melakukan estimasi hasil perbaikan dari sisi biaya. Penelitian ini menggunakan metode Overall Equipment Effectiveness (OEE dan Fuzzy Failure Mode and Effects Analysis. Hasil penelitian menunjukkan bahwa rata-rata nilai OEE pada tahun 2015 sekitar 52,05%, masih di bawah standar nilai OEE sebesar 85%. Penyebab utamanya adalah adanya gangguan belt sobek karena gesekan belt dengan support return ketika belt conveyor mengalami jogging yang berdampak pada terganggunya penyaluran abu. Modifikasi dapat menghindari kerugian perusahaan sebesar Rp. 582.548.800,00.

  13. Advanced glycation end-products (AGES) and heart failure : Pathophysiology and clinical implications

    NARCIS (Netherlands)

    Hartog, Jasper W. L.; Voors, Adriaan A.; Bakker, Stephan J. L.; Smit, Andries J.; van Veldhuisen, Dirk J.

    2007-01-01

    Advanced glycation end-products (AGEs) are molecules formed during a non-enzymatic reaction between proteins and sugar residues, called the Maillard reaction. AGEs accumulate in the human body with age, and accumulation is accelerated in the presence of diabetes mellitus. In patients with diabetes,

  14. Analysis of failure of voice production by a sound-producing voice prosthesis

    NARCIS (Netherlands)

    van der Torn, M.; van Gogh, C.D.L.; Verdonck-de Leeuw, I M; Festen, J.M.; Mahieu, H.F.

    OBJECTIVE: To analyse the cause of failing voice production by a sound-producing voice prosthesis (SPVP). METHODS: The functioning of a prototype SPVP is described in a female laryngectomee before and after its sound-producing mechanism was impeded by tracheal phlegm. This assessment included:

  15. The FDA's failure to address the lack of generalisability of antidepressant efficacy trials in product labelling.

    Science.gov (United States)

    Zimmerman, Mark

    2016-06-01

    According to the US Food and Drug Administration's (FDA's) regulations, the criteria used to select patients into registration studies should be addressed in a product's label. The FDA's labelling guidelines, which specifically indicate that the routine exclusion of patients of a certain level of severity should be noted in the label, has been uniformly ignored. © The Royal College of Psychiatrists 2016.

  16. Delineating sites of failure following post-prostatectomy radiation treatment using 68Ga-PSMA-PET.

    Science.gov (United States)

    Byrne, Keelan; Eade, Thomas; Kneebone, Andrew; Guo, Linxin; Hsiao, Ed; Schembri, Geoff; Kwong, Carolyn; Hunter, Julia; Emmett, Louise; Hruby, George

    2018-02-01

    To identify sites of failure with 68 Ga-PSMA-PET (PSMA-PET) imaging in patients who have Biochemical Failure (BF) following post-prostatectomy radiotherapy. Between June 2006 and January 2016, 409 men received post prostatectomy intensity modulated radiation treatment (IMRT) with protocolised planning. 310 patients received radiation treatment (RT) to the Prostate Fossa (PF) alone and 99 patients received RT to PF and pelvic lymphatics (PF + LN) usually in combination with androgen deprivation (AD) therapy. Any failure not detected on conventional imaging was delineated with PSMA-PET scanning. Sites of failure were characterised as in-field (PF ± LN), or out of field (nodal alone, distant metastatic alone (visceral or bone) or multi-site failure). Nodal failure was further divided into pelvic failure and/or distant failure. 119 men developed BF, defined as a PSA rise of >0.2 or greater, above post-RT nadir. Freedom from BF was 71% in the PF group and 70% in the PF + LN group, with median follow up of 52 and 44 months respectively. AD was used concomitantly in 13% of the PF group and 92% of the PF + LN group. 81 patients with BF (68%) had PSMA-PET imaging performed as per study intent, 67 (80%) of whom had PSMA avid disease identified. PSMA-PET delineated in-field failure occurred in 2/50 (4%) of the PF group and 1/17 (6%) in the PF + LN group. Nodal failure alone was 33/50 (66%) for the PF group vs 7/17 (41%) for the PF + LN group. For the nodal only failure patients, 18/33 (55%) had pelvic-only nodal failure in the PF group compared to 1/7 (14%) in the PF + LN group (p = 0.03). 16 (32%) of the PSMA avid failures in the PF group would have been encompassed by standard pelvic lymphatic radiotherapy volumes. Post-prostatectomy radiation treatment resulted in excellent in-field control rates. Isolated pelvic nodal failure was rare in those receiving radiotherapy to the prostatic fossa and pelvic nodes but accounted for one third of

  17. Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation.

    Science.gov (United States)

    Onyilo, Francis; Tusiime, Geoffrey; Chen, Li-Hung; Falk, Bryce; Stergiopoulos, Ioannis; Tripathi, Jaindra N; Tushemereirwe, Wilberforce; Kubiriba, Jerome; Changa, Charles; Tripathi, Leena

    2017-01-01

    Black Sigatoka disease, caused by Pseudocercospora fijiensis is a serious constraint to banana production worldwide. The disease continues to spread in new ecological niches and there is an urgent need to develop strategies for its control. The high osmolarity glycerol (HOG) pathway in Saccharomyces cerevisiae is well known to respond to changes in external osmolarity. HOG pathway activation leads to phosphorylation, activation and nuclear transduction of the HOG1 mitogen-activated protein kinases (MAPKs). The activated HOG1 triggers several responses to osmotic stress, including up or down regulation of different genes, regulation of protein translation, adjustments to cell cycle progression and synthesis of osmolyte glycerol. This study investigated the role of the MAPK-encoding PfHog1 gene on osmotic stress adaptation and virulence of P. fijie nsis. RNA interference-mediated gene silencing of PfHog1 significantly suppressed growth of P. fijiensis on potato dextrose agar media supplemented with 1 M NaCl, indicating that PfHog1 regulates osmotic stress. In addition, virulence of the PfHog1 -silenced mutants of P. fijiensis on banana was significantly reduced, as observed from the low rates of necrosis and disease development on the infected leaves. Staining with lacto phenol cotton blue further confirmed the impaired mycelial growth of the PfHog1 in the infected leaf tissues, which was further confirmed with quantification of the fungal biomass using absolute- quantitative PCR. Collectively, these findings demonstrate that PfHog1 plays a critical role in osmotic stress regulation and virulence of P. fijiensis on its host banana. Thus, PfHog1 could be an interesting target for the control of black Sigatoka disease in banana.

  18. Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation

    Directory of Open Access Journals (Sweden)

    Francis Onyilo

    2017-05-01

    Full Text Available Black Sigatoka disease, caused by Pseudocercospora fijiensis is a serious constraint to banana production worldwide. The disease continues to spread in new ecological niches and there is an urgent need to develop strategies for its control. The high osmolarity glycerol (HOG pathway in Saccharomyces cerevisiae is well known to respond to changes in external osmolarity. HOG pathway activation leads to phosphorylation, activation and nuclear transduction of the HOG1 mitogen-activated protein kinases (MAPKs. The activated HOG1 triggers several responses to osmotic stress, including up or down regulation of different genes, regulation of protein translation, adjustments to cell cycle progression and synthesis of osmolyte glycerol. This study investigated the role of the MAPK-encoding PfHog1 gene on osmotic stress adaptation and virulence of P. fijiensis. RNA interference-mediated gene silencing of PfHog1 significantly suppressed growth of P. fijiensis on potato dextrose agar media supplemented with 1 M NaCl, indicating that PfHog1 regulates osmotic stress. In addition, virulence of the PfHog1-silenced mutants of P. fijiensis on banana was significantly reduced, as observed from the low rates of necrosis and disease development on the infected leaves. Staining with lacto phenol cotton blue further confirmed the impaired mycelial growth of the PfHog1 in the infected leaf tissues, which was further confirmed with quantification of the fungal biomass using absolute- quantitative PCR. Collectively, these findings demonstrate that PfHog1 plays a critical role in osmotic stress regulation and virulence of P. fijiensis on its host banana. Thus, PfHog1 could be an interesting target for the control of black Sigatoka disease in banana.

  19. Fission product release assessment for end fitting failure in Candu reactor loaded with CANFLEX-NU fuel bundles

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Dirk Joo; Jeong, Chang Joon; Lee, Kang Moon; Suk, Ho Chun [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1997-12-31

    Fission product release (FPR) assessment for End Fitting Failure (EFF) in CANDU reactor loaded with CANFLEX-natural uranium (NU) fuel bundles has been performed. The predicted results are compared with those for the reactor loaded with standard 37-element bundles. The total channel I-131 release at the end of transient for EFF accident is calculated to be 380.8 TBq and 602.9 TBq for the CANFLEX bundle and standard bundle channel cases, respectively. They are 4.9% and 7.9% of total inventory, respectively. The lower total releases of the CANFLEX bundle O6 channel are attributed to the lower initial fuel temperatures caused by the lower linear element power of the CANFLEX bundle compared with the standard bundle. 4 refs., 1 fig., 4 tabs. (Author)

  20. Fission product release assessment for end fitting failure in Candu reactor loaded with CANFLEX-NU fuel bundles

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Dirk Joo; Jeong, Chang Joon; Lee, Kang Moon; Suk, Ho Chun [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1998-12-31

    Fission product release (FPR) assessment for End Fitting Failure (EFF) in CANDU reactor loaded with CANFLEX-natural uranium (NU) fuel bundles has been performed. The predicted results are compared with those for the reactor loaded with standard 37-element bundles. The total channel I-131 release at the end of transient for EFF accident is calculated to be 380.8 TBq and 602.9 TBq for the CANFLEX bundle and standard bundle channel cases, respectively. They are 4.9% and 7.9% of total inventory, respectively. The lower total releases of the CANFLEX bundle O6 channel are attributed to the lower initial fuel temperatures caused by the lower linear element power of the CANFLEX bundle compared with the standard bundle. 4 refs., 1 fig., 4 tabs. (Author)

  1. Analysis of the PLC object model in the PF linac

    International Nuclear Information System (INIS)

    Abe, Isamu; Shirakawa, Akihiro; Nakahara, Kazuo; Tanaka, Masahiko

    1995-01-01

    Device controllers (front end) for the KEK PF Linac, which has been operated for more than ten years, had to be renewed, because there is no longer support from hardware manufacturer. To reduce maintenance costs, device controllers are planed to be replaced by PLC (Programmable Logic Controller). Investigations launched in the late 1994s for the PLC have carried out in order to make sure of the I/O stability and network possibilities. In this paper, the software for the PLC is discussed based on the OOP concept. (author)

  2. Current distribution between petals in PF-FSJS sample

    International Nuclear Information System (INIS)

    Zani, L.

    2003-01-01

    6 Rogowski coils have been installed on each leg of each of the 12 petals in the PF-FSJS sample (poloidal field - full size joint sample) in order to diagnostic current. It appears that Rogowski signal seem reliable for current distribution analysis (Ampere's law is checked and reproducibility is assured) but there is some limitations for qualitative diagnostics. In the series of transparencies results are detailed for the PU1 position, for both leg and right legs and for various unique-angle shift (Δθ) configurations but only results for 0 < Δθ < -5 are consistent

  3. PF-WFS Shell Inspection Update December 2016

    Energy Technology Data Exchange (ETDEWEB)

    Vigil, Anthony Eugene [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Ledoux, Reina Rebecca [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Gonzales, Antonio R. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Montano, Joshua Daniel [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Savage, Lowell Curtis [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Randles, Wayne Alfred [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-01-26

    Since the last project update in FY16:Q2, PF-WFS personnel have advanced in understanding of shell inspection on Coordinate Measuring Machines {CMM} and refined the PF-WFS process to the point it was decided to convert shell inspection from the Sheffield #1 gage to Lietz CM Ms. As a part of introspection on the quality of this process many sets of data have been reviewed and analyzed. This analysis included Sheffield to CMM comparisons, CMM inspection repeatability, fixturing differences, quality check development, probing approach changes. This update report will touch on these improvements that have built the confidence in this process to mainstream it inspecting shells. In addition to the CMM programming advancements, the continuation in refinement of input and outputs for the CMM program has created an archiving scheme, input spline files, an output metafile, and inspection report package. This project will continue to mature. Part designs may require program modifications to accommodate "new to this process" part designs. Technology limitations tied to security and performance are requiring possible changes to computer configurations to support an automated process.

  4. Production, soil erosion and economic failure in new citrus plantations in Eastern Spain

    Science.gov (United States)

    Giménez Morera, Antonio; Carles membrado, Joan; Cerdà, Artemi; Ángel González Peñaloza, Félix

    2013-04-01

    Eastern Spain has been worldwide well known by the high quality citrus production (Piqueras, 2012). During the last century, the export of València's oranges contributed to a high income in Spain albeit during the last decade the revenues for the small farmers were short (Bono, 2010). The orange agricultural specialization in València begun at the end of the eighteenth century in the town of Carcaixent, close to the Xúquer river, where the first commercial orange groves were planted. This was due to the climatic conditions (no frosts) and the traditional flood irrigation systems. The orange trade was not important until the second half of the nineteenth century, due to a combination of factors: i) the increasing demand of oranges from the United Kingdom, first, and then from Germany, France and other north-european industrialized countries; ii) the highly productive capacity of the Valencian soil thanks to its mild weather and irrigated fields; iii) the open mindedness of Valencian farmers towards innovation; and, iv) the developemnt of a railways network which made it possible to bring the oranges into the shipment ports (Bono, 2010; Piqueras, 2012). The Valencian orange trade knew its peak during the period 1925-1930 but later it experienced an economic crisis because of wars (both in Spain and Europe) and did not recover until the 1960's (Piqueras, 1999; Bono, 2010). After Spain's EEC (European Economic Community) membership (1986) and the creation of EU (1993) Valencian citrus sector grew: new orange groves were planted, new commercial varieties (especially mandarins) were promoted, and exports increased. Nevertheless, nowadays Valencian orange sector suffers from a structural problem: the small farm size and the lack of a good commercial network of distribution. But in spite of the current crisis, the orange groves' impact on landscape is still huge in Valencia, since it creates a thick forest of orange trees stretching not only the floodplains but also

  5. Targeting the CXCR4 pathway using a novel anti-CXCR4 IgG1 antibody (PF-06747143 in chronic lymphocytic leukemia

    Directory of Open Access Journals (Sweden)

    Manoj K. Kashyap

    2017-05-01

    Full Text Available Abstract Background The CXCR4-CXCL12 axis plays an important role in the chronic lymphocytic leukemia (CLL-microenvironment interaction. Overexpression of CXCR4 has been reported in different hematological malignancies including CLL. Binding of the pro-survival chemokine CXCL12 with its cognate receptor CXCR4 induces cell migration. CXCL12/CXCR4 signaling axis promotes cell survival and proliferation and may contribute to the tropism of leukemia cells towards lymphoid tissues and bone marrow. Therefore, we hypothesized that targeting CXCR4 with an IgG1 antibody, PF-06747143, may constitute an effective therapeutic approach for CLL. Methods Patient-derived primary CLL-B cells were assessed for cytotoxicity in an in vitro model of CLL microenvironment. PF-06747143 was analyzed for cell death induction and for its potential to interfere with the chemokine CXCL12-induced mechanisms, including migration and F-actin polymerization. PF-06747143 in vivo efficacy was determined in a CLL murine xenograft tumor model. Results PF-06747143, a novel-humanized IgG1 CXCR4 antagonist antibody, induced cell death of patient-derived primary CLL-B cells, in presence or absence of stromal cells. Moreover, cell death induction by the antibody was independent of CLL high-risk prognostic markers. The cell death mechanism was dependent on CXCR4 expression, required antibody bivalency, involved reactive oxygen species production, and did not require caspase activation, all characteristics reminiscent of programmed cell death (PCD. PF-06747143 also induced potent B-CLL cytotoxicity via Fc-driven antibody-dependent cell-mediated cytotoxicity (ADCC and complement-dependent cytotoxicity activity (CDC. PF-06747143 had significant combinatorial effect with standard of care (SOC agents in B-CLL treatment, including rituximab, fludarabine (F-ara-A, ibrutinib, and bendamustine. In a CLL xenograft model, PF-06747143 decreased tumor burden and improved survival as a monotherapy

  6. Identification of a Signal That Mediates the Crosstalk Between Biosynthetic Gene Clusters for the Antibiotics 2,4-diacetylphloroglucinol and Pyoluteorin in Pseudomonas protegens Pf-5

    Science.gov (United States)

    Pseudomonas protegens Pf-5 produces a broad spectrum of secondary metabolites with anti-microbial activity. The production of two of these metabolites, 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin, is coordinately regulated. Our previous study indicated that phloroglucinol, an intermediate in t...

  7. Fungal-specific transcription factor AbPf2 activates pathogenicity in Alternaria brassicicola

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Yangrae; Ohm, Robin A. [US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA; Grigoriev, Igor V. [US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA; Srivastava, Akhil [Plant and Environmental Protection Sciences, University of Hawaii at Manoa, 3190 Maile Way, St John 317, Honolulu, HI, 96822, USA

    2013-05-24

    Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. To identify molecular determinants of pathogenicity, we created non-pathogenic mutants of a transcription factor-encoding gene, AbPf2. The frequency and timing of germination and appressorium formation on host plants were similar between the non-pathogenic abpf2 mutants and wild-type A. brassicicola. The mutants were also similar in vitro to wild-type A. brassicicola in terms of vegetative growth, conidium production, and responses to a phytoalexin, reactive oxygen species and osmolites. The hyphae of the mutants grew slowly but did not cause disease symptoms on the surface of host plants. Transcripts of the AbPf2 gene increased exponentially soon after wild-type conidia contacted their host plants . A small amount of AbPf2 protein, as monitored using GFP fusions, was present in young, mature conidia. The protein level decreased during saprophytic growth, but increased and was located primarily in fungal nuclei during pathogenesis. Levels of the proteins and transcripts sharply decreased following colonization of host tissues beyond the initial infection site. When expression of the transcription factor was induced in the wild-type during early pathogenesis, 106 fungal genes were also induced in the wild-type but not in the abpf2 mutants. Notably, 33 of the 106 genes encoded secreted proteins, including eight putative effector proteins. Plants inoculated with abpf2 mutants expressed higher levels of genes associated with photosynthesis, the pentose phosphate pathway and primary metabolism, but lower levels of defense-related genes. Our results suggest that AbPf2 is an important regulator of pathogenesis, but does not affect other cellular processes in A. brassicicola.

  8. Private Finance 2 (PF2): Re-inventing the Wheel?

    Science.gov (United States)

    Zawawi, N. A. W. A.; Abdul-Aziz, A. R.; Khamidi, M. F.; Othman, I.; Idrus, A.; Umar, A. A.

    2013-06-01

    The Procurement policy of any government is the most influential factor in determining the efficiency of infrastructure and service provision like roads, water supply and energy. The UK's HM Treasury released its new guidelines on private involvement in infrastructures provision and services towards reforming the popular Private Finance Initiatives (PFI) policy. This new approach, it now refers to as the Private Finance 2 (PF2) is meant to correct the imperfections which have bedeviled the older version-PFI. However, the 'new guidelines' contained nothing really new in the area of private financing and operation of public infrastructures, at best it is akin to 're-inventing the wheel' rather than being 'new'. While dwelling extensively on issues relating to cheaper financing sources, risks transfer, counterpart funding by government and improving public sector procurement skills, this paper argues that some countries in the developing world have long recognised these issues and taken practical steps to correct them.

  9. Private Finance 2 (PF2): Re-inventing the Wheel?

    International Nuclear Information System (INIS)

    Zawawi, N A W A; Khamidi, M F; Othman, I; Umar, A A; Abdul-Aziz, A R; Idrus, A

    2013-01-01

    The Procurement policy of any government is the most influential factor in determining the efficiency of infrastructure and service provision like roads, water supply and energy. The UK's HM Treasury released its new guidelines on private involvement in infrastructures provision and services towards reforming the popular Private Finance Initiatives (PFI) policy. This new approach, it now refers to as the Private Finance 2 (PF2) is meant to correct the imperfections which have bedeviled the older version-PFI. However, the 'new guidelines' contained nothing really new in the area of private financing and operation of public infrastructures, at best it is akin to 're-inventing the wheel' rather than being 'new'. While dwelling extensively on issues relating to cheaper financing sources, risks transfer, counterpart funding by government and improving public sector procurement skills, this paper argues that some countries in the developing world have long recognised these issues and taken practical steps to correct them.

  10. Lactulose efficacy in reduction of nitrogen products, blood potassium and fluid overload in patients with end-stage renal failure

    Directory of Open Access Journals (Sweden)

    Negin Aleagha

    2017-06-01

    Full Text Available Introduction: Chronic kidney disease (CKD is a major public health problem that often goes unrecognized until its late-stage. Patients with chronic kidney disease face uremic toxins and hyperkalemia. Also, fluid overload in CKD patients is associated with rapid decline in kidney function. Lactulose is a hyperosmotic agent and as a prebiotic, it plays an important role in regulating serum urea and potassium levels and has some effects on fluid overload. The aim of this study was to evaluate the effect of lactulose on serum levels of biochemical products in patients with CKD. Materials and Methods: In this interventional study, 17 patients with end stage of CKD ( 76.47 % men; mean age 65.88 ± 13.4 were evaluated.All patients received lactulose, 10 ml, 3 times per day for 3 months. Blood samples from all participants were collected before and at the end of intervention to examine changes in biochemical parameters, including potassium, urea, creatinine and uric acid. Results: Lactulose significantly decreased urea levels (p=0.001, blood potassium (0.001 and fluid overload(considering the patient’s weight p=0.001 in patients with end-stage renal failure. The decrease in serum creatinine and uric acid were not significant. Conclusion: Lactulose administration in CKD patients could decrease levels of various deleterious elements, especially urea and blood potassium and its daily use can be recommended in these patients.

  11. Patterns of failure following treatment for medulloblastoma: is it necessary to treat the entire posterior fossa?

    International Nuclear Information System (INIS)

    Fukunaga-Johnson, Nina; Lee, Jason H.; Robertson, Patricia; Sandler, Howard M.; McNeil, Elizabeth; Goldwein, Joel W.

    1997-01-01

    Purpose: Craniospinal radiation (CSRT) followed by a boost to the entire posterior fossa (PF) is standard post-operative therapy for patients with medulloblastoma. A large proportion of recurrences after treatment are local with approximately 50-70% of recurrences occurring in the PF. It is unclear, however, whether these failures are occurring in the original tumor bed, or outside the tumor bed but still within the PF. With improved diagnostic imaging, better definition of tumor volumes, and the use of 3D conformal therapy, we may be able to restrict the boost volume to the tumor bed plus a margin without compromising local control. This retrospective study analyzes the patterns of failure within the PF of a series of patients treated with radiation therapy (RT). Methods: From (7(86)) through (2(96)), 27 of 114 (24%) patients > 18 mo and < 18 yr with medulloblastoma, who were treated at the University of Michigan and Children's Hospital of Philadelphia, with RT following surgical resection were found to have a recurrence. RT consisted of CSRT followed by a boost to the entire posterior fossa. Patient's preoperative MRI and/or CT studies were used to compare the original tumor volume with the specific region of local relapse. Failure was defined as MRI or CT evidence of recurrence or positive cerebrospinal fluid cytology. Relapse was scored as local, if it was within the original tumor bed and regional, if it was outside of the tumor bed but still within the PF. Results: The median age of the patients was 8.6 years. Three patients were < 3 years old. (21(27)) had disease localized to the PF. (19(21)) patients received chemotherapy during their treatment regimen; 6 patients did not have information on systemic treatment. The median dose of RT to the craniospinal axis was 32.5 Gy and to the PF was 55.2 Gy. The median time to recurrence was 19.5 months. Local failure within the tumor bed as any component of first failure occurred in 52% ((14(27))) of all failures but

  12. Paracheck-PF ® test Versus Microscopy in the Diagnosis of ...

    African Journals Online (AJOL)

    Background: Malaria is a major cause of morbidity and mortality in Ethiopia. Rapid diagnostic tests such as Paracheck Pf are the major tools for falciparum malaria diagnosis as an alternative to microscopy in peripheral health facilities. The objective of this study was to evaluate the sensitivity and specificity of Paracheck Pf ...

  13. Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas protegens Pf-5

    Science.gov (United States)

    Hartney, Sierra L.; Mazurier, Sylvie; Girard, Maëva K.; Mehnaz, Samina; Davis, Edward W.; Gross, Harald; Lemanceau, Philippe

    2013-01-01

    The soil bacterium Pseudomonas protegens Pf-5 (previously called P. fluorescens Pf-5) produces two siderophores, enantio-pyochelin and a compound in the large and diverse pyoverdine family. Using high-resolution mass spectroscopy, we determined the structure of the pyoverdine produced by Pf-5. In addition to producing its own siderophores, Pf-5 also utilizes ferric complexes of some pyoverdines produced by other strains of Pseudomonas spp. as sources of iron. Previously, phylogenetic analysis of the 45 TonB-dependent outer membrane proteins in Pf-5 indicated that six are in a well-supported clade with ferric-pyoverdine receptors (Fpvs) from other Pseudomonas spp. We used a combination of phylogenetics, bioinformatics, mutagenesis, pyoverdine structural determinations, and cross-feeding bioassays to assign specific ferric-pyoverdine substrates to each of the six Fpvs of Pf-5. We identified at least one ferric-pyoverdine that was taken up by each of the six Fpvs of Pf-5. Functional redundancy of the Pf-5 Fpvs was also apparent, with some ferric-pyoverdines taken up by all mutants with a single Fpv deletion but not by a mutant having deletions in two of the Fpv-encoding genes. Finally, we demonstrated that phylogenetically related Fpvs take up ferric complexes of structurally related pyoverdines, thereby establishing structure-function relationships that can be employed in the future to predict the pyoverdine substrates of Fpvs in other Pseudomonas spp. PMID:23222724

  14. ESR study of weakly irradiated organic conductors: TMTSF-DMTCNQ and (TMTSF)2PF6

    International Nuclear Information System (INIS)

    Forro, L.; Beuneu, F.

    1982-01-01

    ESR experiments are presented on irradiated TMTSF-DMTCNQ and (TMTSF) 2 PF 6 . They suggest that a weak disorder extends the metallic phases to low temperatures. Surprisingly, disorder has no effect on the ESR linewidth of (TMTSF) 2 PF 6 between 20 and 30 K, where the d.c. conductivity changes a lot with disorder. (author)

  15. A Variant PfCRT Isoform Can Contribute to Plasmodium falciparum Resistance to the First-Line Partner Drug Piperaquine

    Directory of Open Access Journals (Sweden)

    Satish K. Dhingra

    2017-05-01

    Full Text Available Current efforts to reduce the global burden of malaria are threatened by the rapid spread throughout Asia of Plasmodium falciparum resistance to artemisinin-based combination therapies, which includes increasing rates of clinical failure with dihydroartemisinin plus piperaquine (PPQ in Cambodia. Using zinc finger nuclease-based gene editing, we report that addition of the C101F mutation to the chloroquine (CQ resistance-conferring PfCRT Dd2 isoform common to Asia can confer PPQ resistance to cultured parasites. Resistance was demonstrated as significantly higher PPQ concentrations causing 90% inhibition of parasite growth (IC90 or 50% parasite killing (50% lethal dose [LD50]. This mutation also reversed Dd2-mediated CQ resistance, sensitized parasites to amodiaquine, quinine, and artemisinin, and conferred amantadine and blasticidin resistance. Using heme fractionation assays, we demonstrate that PPQ causes a buildup of reactive free heme and inhibits the formation of chemically inert hemozoin crystals. Our data evoke inhibition of heme detoxification in the parasite’s acidic digestive vacuole as the primary mode of both the bis-aminoquinoline PPQ and the related 4-aminoquinoline CQ. Both drugs also inhibit hemoglobin proteolysis at elevated concentrations, suggesting an additional mode of action. Isogenic lines differing in their pfmdr1 copy number showed equivalent PPQ susceptibilities. We propose that mutations in PfCRT could contribute to a multifactorial basis of PPQ resistance in field isolates.

  16. Spectrophotometric assessment of the effectiveness of Opalescence PF 10%: a 14-month clinical study.

    Science.gov (United States)

    Grobler, S R; Hayward, R; Wiese, S; Moola, M H; van W Kotze, T J

    2010-02-01

    To evaluate the effectiveness of Opalescence PF 10% just after treatment, at 6-month follow-up and at a 14-month follow-up period. Opalescence PF 10% was applied nightly for 14 days. The color of teeth 11 and 21 of 17 subjects were measured with a spectrophotometer (L*; a*; b*) before treatment, just after treatment (14 days), after 6 months and after 14 months. Subjects were instructed to take note of any tooth sensitivity. For all three components (L*, a* and b*) statistical significant differences (p<0.05) in the values between base-line, after treatment (14 days later), after 6 months and after 14 months were found (Wilcoxon Signed Rank Sum Test). The decrease in L* was about 20% after 6 months and about 50% after 14 months. The a* value decreased approximately 14% after 6 months but was worse after 14 months than at the beginning. The b* value decreased the least with about 9% after 6 months and about 8% after 14 months. The decrease in DeltaE(ab)(*) was approximately 20% after 14 months. Less than 20% of the subjects experienced mild tooth sensitivity just after treatment. Significantly whiter teeth were found after treatment as well as after a 6-month follow-up period. The whiteness/brightness (L*) decreased with approximately 50% after 14 months and the a* value with approximately 50% after 7 months, while the yellowness (b* value) remained even after 14 months. The product is an effective tooth whitener resulting in only low tooth sensitivity. Re-bleaching could be done at about 14 months. Copyright 2009 Elsevier Ltd. All rights reserved.

  17. Targeting colorectal cancer cells by a novel sphingosine kinase 1 inhibitor PF-543

    Energy Technology Data Exchange (ETDEWEB)

    Ju, TongFa [Department of Anal-colorectal Surgery, HangZhou First People' s Hospital, HangZhou (China); Gao, DaQuan [Hematological Department, HangZhou First People' s Hospital, HangZhou (China); Fang, Zheng-yu, E-mail: fangzhengyu158@sina.com [Department of Anal-colorectal Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou (China)

    2016-02-12

    In this study, we showed that PF-543, a novel sphingosine kinase 1 (SphK1) inhibitor, exerted potent anti-proliferative and cytotoxic effects against a panel of established (HCT-116, HT-29 and DLD-1) and primary human colorectal cancer (CRC) cells. Its sensitivity was negatively associated with SphK1 expression level in the CRC cells. Surprisingly, PF-543 mainly induced programmed necrosis, but not apoptosis, in the CRC cells. CRC cell necrotic death was detected by lactate dehydrogenase (LDH) release, mitochondrial membrane potential (MMP) collapse and mitochondrial P53-cyclophilin-D (Cyp-D) complexation. Correspondingly, the necrosis inhibitor necrostatin-1 largely attenuated PF-543-induced cytotoxicity against CRC cells. Meanwhile, the Cyp-D inhibitors (sanglifehrin A and cyclosporin A), or shRNA-mediated knockdown of Cyp-D, remarkably alleviated PF-543-induced CRC cell necrotic death. Reversely, over-expression of wild-type Cyp-D in HCT-116 cells significantly increased PF-543's sensitivity. In vivo, PF-543 intravenous injection significantly suppressed HCT-116 xenograft growth in severe combined immunodeficient (SCID) mice, whiling remarkably improving the mice survival. The in vivo activity by PF-543 was largely attenuated when combined with the Cyp-D inhibitor cyclosporin A. Collectively, our results demonstrate that PF-543 exerts potent anti-CRC activity in vitro and in vivo. Mitochondrial programmed necrosis pathway is likely the key mechanism responsible for PF-543's actions in CRC cells. - Highlights: • PF-543 is anti-proliferative and cytotoxic to established and primary CRC cells. • PF-543 induces programmed necrosis, but not apoptosis, in CRC cells. • Modulation of mitochondrial protein cyclophilin-D alters PF-543's sensitivity. • PF-543 inhibits HCT-116 xenograft growth in SCID mice, improving mice survival. • Co-administration of cyclophilin-D inhibitor CsA inhibits PF-543's activity in vivo.

  18. Targeting colorectal cancer cells by a novel sphingosine kinase 1 inhibitor PF-543

    International Nuclear Information System (INIS)

    Ju, TongFa; Gao, DaQuan; Fang, Zheng-yu

    2016-01-01

    In this study, we showed that PF-543, a novel sphingosine kinase 1 (SphK1) inhibitor, exerted potent anti-proliferative and cytotoxic effects against a panel of established (HCT-116, HT-29 and DLD-1) and primary human colorectal cancer (CRC) cells. Its sensitivity was negatively associated with SphK1 expression level in the CRC cells. Surprisingly, PF-543 mainly induced programmed necrosis, but not apoptosis, in the CRC cells. CRC cell necrotic death was detected by lactate dehydrogenase (LDH) release, mitochondrial membrane potential (MMP) collapse and mitochondrial P53-cyclophilin-D (Cyp-D) complexation. Correspondingly, the necrosis inhibitor necrostatin-1 largely attenuated PF-543-induced cytotoxicity against CRC cells. Meanwhile, the Cyp-D inhibitors (sanglifehrin A and cyclosporin A), or shRNA-mediated knockdown of Cyp-D, remarkably alleviated PF-543-induced CRC cell necrotic death. Reversely, over-expression of wild-type Cyp-D in HCT-116 cells significantly increased PF-543's sensitivity. In vivo, PF-543 intravenous injection significantly suppressed HCT-116 xenograft growth in severe combined immunodeficient (SCID) mice, whiling remarkably improving the mice survival. The in vivo activity by PF-543 was largely attenuated when combined with the Cyp-D inhibitor cyclosporin A. Collectively, our results demonstrate that PF-543 exerts potent anti-CRC activity in vitro and in vivo. Mitochondrial programmed necrosis pathway is likely the key mechanism responsible for PF-543's actions in CRC cells. - Highlights: • PF-543 is anti-proliferative and cytotoxic to established and primary CRC cells. • PF-543 induces programmed necrosis, but not apoptosis, in CRC cells. • Modulation of mitochondrial protein cyclophilin-D alters PF-543's sensitivity. • PF-543 inhibits HCT-116 xenograft growth in SCID mice, improving mice survival. • Co-administration of cyclophilin-D inhibitor CsA inhibits PF-543's activity in vivo.

  19. Determination of bounds on failure probability in the presence of ...

    Indian Academy of Sciences (India)

    In particular, fuzzy set theory provides a more rational framework for ..... indicating that the random variations inT andO2 do not affect failure probability significantly. ... The upper-bound for PF shown in figure 6 can be used in decision-making.

  20. Design report on PF injector linac upgrade for KEKB

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Isamu; Anami, Shozo; Enomoto, Atsushi; Fukuda, Shigeki; Kobayashi, Hitoshi; Nakahara, Kazuo [eds.

    1966-03-01

    The purpose of the B Factory project is to verify the physical problem `Is there difference in the physical laws of particle world and anti-particle world?` The outline of the KEK B Factory project (KEKB) is explained. The condition of injection corresponding to the KEKB, the increase of the energy of the PF injector and the augmentation of positron beam intensity for the KEKB, the guideline for, the most important problems of and the schedule of energy augmentation are described. Buildings and utilities, various problems related to large current electron beam acceleration, the generation of positrons, the examination of acceleration method, beam transport system, acceleration unit, vacuum system, high frequency source, the high frequency phase control between beam and acceleration high frequency wave, electron beam injection system, trigger system, beam monitors, the precision alignment of acceleration tube, electromagnets and beam monitors, the extension of control system, rise and adjustment, and radiation safety and the application related to radiation are described. Efforts are exerted for the development of klystron, the capability of high frequency power compression system, and the withstanding to pressure of acceleration tube. (K.I.)

  1. PF120916 Piecki fireball and Reszel meteorite fall

    Science.gov (United States)

    Olech, A.; Żołądek, P.; Tymiński, Z.; Stolarz, M.; Wiśniewski, M.; Bęben, M.; Lewandowski, T.; Polak, K.; Raj, A.; Zaręba, P.

    2017-06-01

    On September 12, 2016, at 21:44:07 UT, a -9.2±0.5 mag fireball appeared over northeastern Poland. The precise orbit and atmospheric trajectory of the event are presented, based on the data collected by six video stations of the Polish Fireball Network (PFN). The PF120916 Piecki fireball entered the Earth's atmosphere with the velocity of 16.7±0.3 km/s and started to shine at a height of 81.9 ± 0.3 km. Clear deceleration started after first three seconds of the flight, and the terminal velocity of the meteor was only 5.0±0.3 km/s at a height of 26.0 ± 0.2 km. Such a low value of the terminal velocity indicates that fragments with the total mass of around 10-15 kg could survive the atmospheric passage and cause fall of the meteorites. The predicted area of possible meteorite impact is computed and it is located south of Reszel city at the Warmian-Masurian region. The impact area was extensively searched by experienced groups of meteorite hunters, but without any success.

  2. TRAC-PF1/MOD1 computer code

    International Nuclear Information System (INIS)

    Liles, D.R.; Mahaffy, J.H.

    1984-01-01

    The TRAC-P1 program was designed primarily for the analysis of large-break loss-of-coolant accidents (LOCAs) in pressurized water reactors (PWRs). Because of its versatility, however, it can be applied directly to many analyses ranging from blowdowns in simple pipes to integral LOCA tests in multiloop facilities. A refined version, called TRAC-P1A, was released to the National Energy Software Center (NESC) in March 1979. Although it still treats the same class of problems, TRAC-P1A is more efficient than TRAC-P1 and incorporates improved hydrodynamic and heat-transfer models. It also is easier to implement on various computers. TRAC-PD2 contains improved reflood and heat-transfer models and improvements in the numerical solution methods. Although a large LOCA code, it has been applied successfully to small-break problems and to the Three Mile Island incident. Distinguishing characteristics of the TRAC-PF1/MOD1 are summarized

  3. Plasmodium falciparum chloroquine resistance transporter (PfCRT) isoforms PH1 and PH2 perturb vacuolar physiology

    OpenAIRE

    Callaghan, Paul S.; Siriwardana, Amila; Hassett, Matthew R.; Roepe, Paul D.

    2016-01-01

    Background Recent work has perfected yeast-based methods for measuring drug transport by the Plasmodium falciparum chloroquine (CQ) resistance transporter (PfCRT). Methods The approach relies on inducible heterologous expression of PfCRT in Saccharomyces cerevisiae yeast. In these experiments selecting drug concentrations are not toxic to the yeast, nor is expression of PfCRT alone toxic. Only when PfCRT is expressed in the presence of CQ is the growth of yeast impaired, due to inward transpo...

  4. Daya Tahan Hidup Pseudomonas putida Strain Pf-20 dalam Beberapa Macam Inokulum

    Directory of Open Access Journals (Sweden)

    Yenny Wuryandari

    2004-07-01

    Full Text Available For any crop-protection agent, an efficient formulation is a necessity to translate laboratory activity into adequate field performance. There are particular challenges to be faced in formulation of biological control agents, because the active ingredient is a living organism that must be kept relatively immobile and inactive while in storage, but quickly resume its  normal metabolic processes once applied to the target site. The objective of the research was  to study survival of Pseudomonas putida strain Pf-20 in various formulations at the storage  time and germination. The twelve formulations include carriers, additives and concentration  of Pf-20. The efficacy of various formulation in maintaining the population of Pf-20 in storage was assessed. The research result showed that population of Pf 20  in the formulation  number seven was the highest, with the combination peat+talc, CMC+arginin and  concentration of Pf[20 10^10 CFU/ml. In peat+talc, CMC+arginin, Pf-20 10^10  CFU/ml based formulation the bacteria survived even up to 84 days of storage although the population declined. In all formulations, population of Pf-20 increased at the time of seed germination. At the time of seed germination, formulation number seven was the highest too.

  5. Evaluation of containment failure modes and fission product releases during core meltdown accidents in a BWR with a Mark III containment

    International Nuclear Information System (INIS)

    Ludewig, H.; Yu, W.S.; Jaung, R.; Pratt, W.T.

    1985-01-01

    An assessment is described of potential failure modes and fission product releases for a large number of postulated core meltdown accidents in a BWR with a Mark III containment. For this containment design, the most important failure mode was found to be due to hydrogen related phenomena. A one-dimensional lumped parameter computer code has been developed and used to determine the probability of various hydrogen phenomena for a range of postulated core meltdown sequences. Potential containment loads have been estimated and compared against the containment capacity to determine the probability of containment failure. The fission product release assessment began by using the MARCH/CORRAL system of codes with key input parameters varied over a reasonable range. The parameters relate to primary system retention, re-emission, pool scrubbing, and fission product release in-vessel vs ex-vessel. The final step used more mechanistic calculations based on the system of codes recently developed under sponsorship of the Accident Source Term Program Office, NRC, and compares these predictions with the range of releases calculated in the sensitivity study

  6. Accuracy of PfHRP2 versus Pf-pLDH antigen detection by malaria rapid diagnostic tests in hospitalized children in a seasonal hyperendemic malaria transmission area in Burkina Faso

    OpenAIRE

    Maltha, Jessica; Guiraud, Issa; Lompo, Palpouguini; Kaboré, Bérenger; Gillet, Philippe; Van Geet, Chris; Tinto, Halidou; Jacobs, Jan

    2014-01-01

    Background In most sub-Saharan African countries malaria rapid diagnostic tests (RDTs) are now used for the diagnosis of malaria. Most RDTs used detect Plasmodium falciparum histidine-rich protein-2 (PfHRP2), though P. falciparum-specific parasite lactate dehydrogenase (Pf-pLDH)-detecting RDTs may have advantages over PfHRP2-detecting RDTs. Only few data are available on the use of RDTs in severe illness and the present study compared Pf-pLDH to PfHRP2-detection. Methods Hospitalized children...

  7. pltM is important in the phloroglucinol-mediated crosstalk between biosynthetic gene clusters for the antibiotics 2,4-diacetylphloroglucinol and pyoluteorin in Pseudomonas protegens Pf-5

    Science.gov (United States)

    Pseudomonas protegens strain Pf-5 is a well-characterized rhizosphere bacterium known for its production of a diverse spectrum of antimicrobial secondary metabolites. The production of two of these metabolites, 2,4-diacetylphloroglucinol (2,4-DAPG) and pyoluteorin, is coordinately regulated. Each of...

  8. Acute Liver Failure

    Science.gov (United States)

    ... can cause acute liver failure. It is an industrial chemical found in refrigerants and solvents for waxes, varnishes ... measures when spraying insecticides, fungicides, paint and other toxic chemicals. Follow product instructions carefully. Watch what gets on ...

  9. Pf16 and phiPMW: Expanding the realm of Pseudomonas putida bacteriophages.

    Directory of Open Access Journals (Sweden)

    Damian J Magill

    Full Text Available We present the analysis of two novel Pseudomonas putida phages, pf16 and phiPMW. Pf16 represents a peripherally related T4-like phage, and is the first of its kind infecting a Pseudomonad, with evidence suggesting cyanophage origins. Extensive divergence has resulted in pf16 occupying a newly defined clade designated as the pf16-related phages, lying at the interface of the Schizo T-Evens and Exo T-Evens. Recombination with an ancestor of the P. putida phage AF is likely responsible for the tropism of this phage. phiPMW represents a completely novel Pseudomonas phage with a genome containing substantial genetic novelty through its many hypothetical proteins. Evidence suggests that this phage has been extensively shaped through gene transfer events and vertical evolution. Phylogenetics shows that this phage has an evolutionary history involving FelixO1-related viruses but is in itself highly distinct from this group.

  10. Implantation of TRAC-PF1 computer code of VAX-11/750

    International Nuclear Information System (INIS)

    Madeira, A.A.; Souza Gouvea, L. de; Galetti, M.R.S.

    1988-01-01

    The implantation of TRAC-PF1 code, IBM version, in VAX-11/750 is described. This work provides Reator Department with an advanced best-estimate tool to perform loss-of-coolant accident analysis. (Author) [pt

  11. Anisotropic p-f mixing mechanism explaining anomalous magnetic properties in Ce monopnictides

    International Nuclear Information System (INIS)

    Takahashi, H.; Kasuya, T.

    1985-01-01

    The crystal-field splittings in CeP, PrP and NdP are calculated by considering the point-charge Coulomb interaction, the intra-atomic d-f Coulomb interaction, and the p-f and d-f mixings. The p-f mixing mechanisms, not only between the occupied 4f states and the conduction bands, but also between the unoccupied 4f states and the valence bands make an important contribution to the crystal-field splitting. The fact that the crystal-field potential in CeP is smaller than those in PrP and NdP is due to the occupied 4f level in CeP being shallower. The values of the Slater-Koster integrals, (pfσ) and (pfπ), are determined uniquely from the crystal-field fitting for PrP and NdP. (author)

  12. [Application study on PHI and 16PF and SCL-90 for freshman's psychology inspection].

    Science.gov (United States)

    Niu, Peng

    2009-07-01

    To explore the effect of application of the measurement table of PHI and 16PF and SCL-90 for freshmen psychology inspection. The measurement tables of PHI and 16PF for psychology inspection of freshmen of 2004-2007 years were used to sift crisis intervention objects. Continuous four years test showed certain stability, in addition to excited factors,the scores of Freshmen's PHI factors were more lower than normal. The incidence rates of mental problems screened by PHI table were very low and 3-5 serious-mental-problem students weren't detected. The problem can be resolved by the application of PHI combined with 16PF through remesuring the suspected cases by SCL-90. The combinative application of PHI, 16PF and SCL-90 would be better.

  13. Industrial engineering studies for the manufacture of the ITER PF coils

    International Nuclear Information System (INIS)

    Libeyre, P.; Decool, P.; Guerin, O.; Perrella, M.; Bourquard, A.

    2007-01-01

    Industrial studies have been carried out in Europe to prepare the manufacture of the five poloidal field (PF) coils, which will be manufactured on the ITER site. A first study, carried out by Ansaldo Superconduttori, addressed the manufacturing sequence, assuming the manufacture of the PF coils inside the two buildings which will further host the cryogenic system. A second study, carried out by Alstom investigated how to achieve the manufacture of some crucial points. A new layout of the manufacturing line was proposed, aiming at manufacture of the PF2-6 coils within 36 months. A recent study performed by Alstom, assumes the manufacture of the PF coils in a single dedicated building, releasing so the constraint of meeting the deadline fixed in ITER reference scheme by the starting point of installation of the cryogenic components

  14. Feasibility analysis of fuzzy logic control for ITER Poloidal field (PF) AC/DC converter system

    Energy Technology Data Exchange (ETDEWEB)

    Hassan, Mahmood Ul; Fu, Peng [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); University of Science and Technology of China (China); Song, Zhiquan, E-mail: zhquansong@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Chen, Xiaojiao [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); University of Science and Technology of China (China); Zhang, Xiuqing [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Humayun, Muhammad [Shanghai Jiaotong University (China)

    2017-05-15

    Highlights: • The implementation of the Fuzzy controller for the ITER PF converter system is presented. • The comparison of the FLC and PI simulation are investigated. • The FLC single and parallel bridge operation are presented. • Fuzzification and Defuzzification algorithms are presented using FLC controller. - Abstract: This paper describes the feasibility analysis of the fuzzy logic control to increase the performance of the ITER poloidal field (PF) converter systems. A fuzzy-logic-based controller is designed for ITER PF converter system, using the traditional PI controller and Fuzzy controller (FC), the dynamic behavior and transient response of the PF converter system are compared under normal operation by analysis and simulation. The analysis results show that the fuzzy logic control can achieve better operation performance than PI control.

  15. PfSETvs methylation of histone H3K36 represses virulence genes in Plasmodium falciparum

    DEFF Research Database (Denmark)

    Jiang, Lubin; Mu, Jianbing; Zhang, Qingfeng

    2013-01-01

    The variant antigen Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), which is expressed on the surface of P. falciparum-infected red blood cells, is a critical virulence factor for malaria. Each parasite has 60 antigenically distinct var genes that each code for a different PfEMP1...... parasite nuclei and their expression as proteins on the surface of individual infected red blood cells. PfSETvs-dependent H3K36me3 is present along the entire gene body, including the transcription start site, to silence var genes. With low occupancy of PfSETvs at both the transcription start site of var...... protein. During infection the clonal parasite population expresses only one gene at a time before switching to the expression of a new variant antigen as an immune-evasion mechanism to avoid the host antibody response. The mechanism by which 59 of the 60 var genes are silenced remains largely unknown...

  16. Respiratory Failure

    Science.gov (United States)

    Respiratory failure happens when not enough oxygen passes from your lungs into your blood. Your body's organs, ... brain, need oxygen-rich blood to work well. Respiratory failure also can happen if your lungs can' ...

  17. Heart Failure

    Science.gov (United States)

    Heart failure is a condition in which the heart can't pump enough blood to meet the body's needs. Heart failure does not mean that your heart has stopped ... and shortness of breath Common causes of heart failure are coronary artery disease, high blood pressure and ...

  18. The evaluation of failure stress and released amount of fission product gas of power ramped rod by fuel behaviour analysis code 'FEMAXI-III'

    International Nuclear Information System (INIS)

    Yanagisawa, Kazuaki; Fujita, Misao

    1984-01-01

    Pellet-Cladding Interaction(PCI) related in-pile failure of Zircaloy sheathed fuel rod is in general considered to be caused by combination of pellet-cladding mechanical interaction(PCMI) with fuel-cladding chemical interaction(FCCI). An understanding of a basic mechanism of PCI-related fuel failure is therefore necessary to get actual cladding hoop stress from mechanical interaction and released amounts of fission product(FP) gas of aggressive environmental agency from chemical interaction. This paper describes results of code analysis performed on fuel failure to cladding hoop stress and amounts of FP gas released under the condition associated with power ramping. Data from Halden(HBWR) and from Studsvik(R2) are used for code analysis. The fuel behaviour analysis code ''FEMAXI-III'' is used as an analytical tool. The followings are revealed from the study: (1) PCI-related fuel failure is dependent upon cladding hoop stress and released amounts of FP gas at power ramping. (2) Preliminary calculated threshold values of hoop stress and of released amounts of FP gas to PCI failure are respectively 330MPa, 10% under the Halden condition, 190MPa, 5% under the Inter ramp(BWR) condition, and 270MPa, 14% under the Over ramp(PWR) condition. The values of hoop stress calculated are almost in the similar range of those obtained from ex-reactor PCI simulated tests searched from references published. (3) The FEMAXI-III code verification is made in mechanical manner by using in-pile deformation data(diametral strain) obtained from power ramping test undertaken by JAERI. While, the code verification is made in thermal manner by using punctured FP gas data obtained from post irradiation examination performed on non-defected power ramped fuel rods. The calculations are resulted in good agreements to both, mechanical and thermal experimental data suggesting the validity of the code evaluation. (J.P.N.)

  19. About the nanostructure of the ternary system water - [BMIm]PF6 - TX-100.

    Science.gov (United States)

    Bilgili, Harun; Bürger, Matthias; Stubenrauch, Cosima; Porada, Jan H

    2016-12-15

    Many efforts have been made to formulate water-IL microemulsions. One of the most intensely studied systems is H 2 O - 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIm]PF 6 ) - 4-octylphenol polyethoxylate (TX-100) and it is not questioned that this system forms microemulsions. The nanostructures observed for traditional microemulsions are postulated with the surfactant being adsorbed at the interface such that the hydrophilic EO sides intrude into the water domains, while the hydrophobic hydrocarbon chains are immersed into [BMIm]PF 6 . However, the high polarity of [BMIm]PF 6 and the observation that [BMIm]PF 6 mixes well with oligoethylene oxides but hardly with non-polar solvents like toluene or alkanes are not in line with this picture. We re-studied the ternary system H 2 O - [BMIm]PF 6 - TX-100 by measuring phase diagrams, determining tie-lines, and carrying out ROESY NMR and PFG NMR measurements. We found that the hydrophobic part of the surfactant interacts neither with water nor with [BMIm]PF 6 , while both solvents interact with the hydrophilic part of the surfactant. We suggest that the surfactant is not adsorbed at the interface between water and the IL, but forms normal spherical or elongated micelles or even continuous aggregates with the hydrocarbon chains forming the interior of the aggregates. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Protection from radiation injury through oral administration of PF4 gene carried by attenuated salmonella

    International Nuclear Information System (INIS)

    Zhao Lihua; Liu Bin; Yu Xiaofei; Zhang Lei; Han Zhongchao

    2005-01-01

    Objective: To investigate the in vivo radiation protection effect of PF4 by oral administration of attenuated salmonella as the carrier in mice. Methods: The eukaryotic vector pIRES2-EGFP-carried PF4 gene was transferred into an aroA-autotrophic mutant of salmonella typhimurium (SL3261), which was administered orally to BALBPc mice at 1x10 8 PFu once every interval three days. At 12 hours after the third oral administration the mice were subjected to a total body irradiation (TBI) of 700 cGy by a 60 Co source. The protective effect of SL3261/PF4 was determined by detection GFP ( green fluorescence protein) expression in tissues, peripheral blood count, culture of bone marrow colony-forming cells and survival time of mice. Results: Expression of GFP could be detected in the liver, spleen, intestine, kidney, peripheral blood and bone marrow. On days 7 and 14 after irradiation, Compared to controls, there were obvious differences in number of bone marrow mononuclear cells, CFU-GM (granulocyte-macrophage colony-forming unit ) and HPP-CFC (high proliferating potential-colony-forming cells) of mice treated with SL3261/PF4 (P<0.05) as well as prolongation of the survival time. Conclusion: These data demonstrate for the first time that PF4 protects mice from TBI injury and accelerates recovery of hematopoiesis by oral administration of attenuated salmonella carrying PF4 gene. (authors)

  1. Application of ISO22000 and Failure Mode and Effect Analysis (fmea) for Industrial Processing of Poultry Products

    Science.gov (United States)

    Varzakas, Theodoros H.; Arvanitoyannis, Ioannis S.

    Failure Mode and Effect Analysis (FMEA) model has been applied for the risk assessment of poultry slaughtering and manufacturing. In this work comparison of ISO22000 analysis with HACCP is carried out over poultry slaughtering, processing and packaging. Critical Control points and Prerequisite programs (PrPs) have been identified and implemented in the cause and effect diagram (also known as Ishikawa, tree diagram and fishbone diagram).

  2. Aluminium cables in automotive applications : Prestudy of aluminium cable uses in Scania products&Failure analysis and evaluation

    OpenAIRE

    Man, Yu

    2016-01-01

    The increasing demand of light constructed vehicles as well as soaring price of copper metal owing to limited nature resources have been promoting the use of aluminium metal as an alternative conductor of automotive cables. This thesis work is to lay theoretical foundations for further research and development regarding the introduction of new automotive cables i.e. aluminium cables. Current application of automotive aluminium cables in automotive industry as well as failure analysis and eval...

  3. Recruitment of PfSET2 by RNA polymerase II to variant antigen encoding loci contributes to antigenic variation in P. falciparum.

    Directory of Open Access Journals (Sweden)

    Uchechi E Ukaegbu

    2014-01-01

    Full Text Available Histone modifications are important regulators of gene expression in all eukaryotes. In Plasmodium falciparum, these epigenetic marks regulate expression of genes involved in several aspects of host-parasite interactions, including antigenic variation. While the identities and genomic positions of many histone modifications have now been cataloged, how they are targeted to defined genomic regions remains poorly understood. For example, how variant antigen encoding loci (var are targeted for deposition of unique histone marks is a mystery that continues to perplex the field. Here we describe the recruitment of an ortholog of the histone modifier SET2 to var genes through direct interactions with the C-terminal domain (CTD of RNA polymerase II. In higher eukaryotes, SET2 is a histone methyltransferase recruited by RNA pol II during mRNA transcription; however, the ortholog in P. falciparum (PfSET2 has an atypical architecture and its role in regulating transcription is unknown. Here we show that PfSET2 binds to the unphosphorylated form of the CTD, a property inconsistent with its recruitment during mRNA synthesis. Further, we show that H3K36me3, the epigenetic mark deposited by PfSET2, is enriched at both active and silent var gene loci, providing additional evidence that its recruitment is not associated with mRNA production. Over-expression of a dominant negative form of PfSET2 designed to disrupt binding to RNA pol II induced rapid var gene expression switching, confirming both the importance of PfSET2 in var gene regulation and a role for RNA pol II in its recruitment. RNA pol II is known to transcribe non-coding RNAs from both active and silent var genes, providing a possible mechanism by which it could recruit PfSET2 to var loci. This work unifies previous reports of histone modifications, the production of ncRNAs, and the promoter activity of var introns into a mechanism that contributes to antigenic variation by malaria parasites.

  4. The effects of pf grind quality on coal burnout in a 1 MW combustion test facility

    Energy Technology Data Exchange (ETDEWEB)

    Richelieu Barranco; Michael Colechin; Michael Cloke; Will Gibb; Edward Lester [University of Nottingham, Nottingham (United Kingdom). School of Chemical, Environmental and Mining Engineering, Nottingham Fuel and Energy Centre

    2006-05-15

    A study was carried out to determine the effect of pf particle size distribution on coal burnout propensity in a 1 MW pulverised fuel burner. The specific aim of the work was to assess the improvement in combustion performance achievable by retrofitting commercially available high performance static or dynamic classifiers to existing plants. Two coals were used and were selected as representative of extremes in fuel characteristics experienced by coal importing utilities in Europe. Each coal was fired in the unit at a range of grind sizes to determine the overall impact of a variable performance from a mill. The levels of unburnt carbon in the resultant flyashes for the two coals showed significantly different behaviour. For the higher volatile coal, the unburnt carbon was found to be insensitive to grind quality. However, the coarser grinds of the other coal produced significantly lower unburnt carbon than expected when compared with the finest grinds. Generally the results indicate that the installation of improved classification technology, leading to a finer product, will help to lower unburnt carbon levels. Nevertheless, further work will be necessary to establish the levels of diminishing returns for grind size, burnout performance and grind costs. 21 refs., 4 figs., 4 tabs.

  5. Optical spectroscopy of free-propagating plasma and its interaction with tungsten targets in PF-1000 facility

    Energy Technology Data Exchange (ETDEWEB)

    Skladnik-Sadowska, E.; Malinowski, K. [The Andrzej Soltan Institute for Nuclear Studies, IPJ, 05-400 Otwock-Swierk (Poland); Sadowski, M.J. [The Andrzej Soltan Institute for Nuclear Studies, IPJ, 05-400 Otwock-Swierk (Poland)] [Institute of Plasma Physics and Laser Microfusion, IPPLM, 01-497 Warsaw (Poland); Kubkowska, M.; Jakubowska, K.; Paduch, M.; Scholz, M. [Institute of Plasma Physics and Laser Microfusion, IPPLM, 01-497 Warsaw (Poland); Garkusha, I.E.; Ladygina, M.; Tereshin, V.I. [Institute of Plasma Physics, NSC KIPT, 61-108 Kharkov (Ukraine)

    2011-07-01

    The paper reports on optical spectroscopy of pulsed plasma streams during their free propagation within a vacuum chamber and their interaction with tungsten targets. Experiments were performed with the PF-1000 facility and particular attention was paid to improvements in spectroscopic diagnostics techniques. In contrary to preliminary studies, the recent spectroscopic measurements of the free plasma streams were carried out perpendicular to the z-axis and at a larger distance from the electrode outlet. The center of the observation quartz-window was located at z = 30 cm in order to observe first a pure deuterium-plasma stream, and later on some heavy impurities which might reach that distance with a delay induced by differences in their production and time-of-flight. The recorded spectral lines were identified by means of a Kurucz database. It was confirmed that at the pure D{sub 2}-filling the PF-1000 facility emits first the deuterium-plasma stream and one can observe intense deuterium Balmer lines, but at a distance z = 30 cm, after about 2 microseconds there appear many impurity lines originating mainly from the Cu-electrodes, i.e. Cu-lines. The second part of the experiment concerned the spectroscopic measurements of metal plasma 'pillow' produced by the plasma stream impinging upon a solid target made of pure tungsten. The described measurements enabled the most intense spectral lines to be identified. This document is composed of an abstract followed by the slides of the presentation

  6. Risk Assessment of Total Coliform in X WTP’s Water Production Using Failure Mode And Effect Analysis Method

    Directory of Open Access Journals (Sweden)

    Bella Apriliani Amanda

    2017-07-01

    Full Text Available The greatest risk of drinking water supply is a failure to provide safe drinking water for communities. Based on IPA Kedunguling testing report on March 2016 noted that sample exceeding the quality standart of Peraturan Menteri Kesehatan RI No 492/2010 for the total coliform quality standart. The presence of total coliforms indicating water contamination by pathogen means the water is not safe to consume. The disinfection process has an importance rule in pathogen inactivation. Disinfectant performance is influenced by temperature, pH, turbidity, and the presence of organic materials. One way to control the quality of water produced by using a risk management approach Failure Modes and Effect Analysis (FMEA methods. The potential risks should be measured to determine causes of the problems and find the appropriate risk reduction. The risk assessment is using Risk Priority Number (RPN scale as a basis prioritization of remedial action on issues. Based on identification and risk analysis using FMEA known that the greatest risk of failure is the stipulation of chlorine dose and organic substances (category of high risk level; residual chlorine (category of moderate risk level; turbidity and pH (very low risk level category. Improvement proposal that can be done to reduce total coliforms presence in IPA Kedunguling is by increasing residual chlorine to 0.6 mg/l, set a daily chlorine level, controlling DBPs forming by lowering the concentration of organic precursor using granular activated carbon (GAC or aeration, by lowering the dose of disinfectant, set aside DBPs after the compound is formed using granular activated carbon (GAC, turbidity and pH monitoring, and regularly washing the filters

  7. Plasmodium falciparum chloroquine resistance transporter (PfCRT) isoforms PH1 and PH2 perturb vacuolar physiology.

    Science.gov (United States)

    Callaghan, Paul S; Siriwardana, Amila; Hassett, Matthew R; Roepe, Paul D

    2016-03-31

    Recent work has perfected yeast-based methods for measuring drug transport by the Plasmodium falciparum chloroquine (CQ) resistance transporter (PfCRT). The approach relies on inducible heterologous expression of PfCRT in Saccharomyces cerevisiae yeast. In these experiments selecting drug concentrations are not toxic to the yeast, nor is expression of PfCRT alone toxic. Only when PfCRT is expressed in the presence of CQ is the growth of yeast impaired, due to inward transport of chloroquine (CQ) via the transporter. During analysis of all 53 known naturally occurring PfCRT isoforms, two isoforms (PH1 and PH2 PfCRT) were found to be intrinsically toxic to yeast, even in the absence of CQ. Additional analysis of six very recently identified PfCRT isoforms from Malaysia also showed some toxicity. In this paper the nature of this yeast toxicity is examined. Data also show that PH1 and PH2 isoforms of PfCRT transport CQ with an efficiency intermediate to that catalyzed by previously studied CQR conferring isoforms. Mutation of PfCRT at position 160 is found to perturb vacuolar physiology, suggesting a fitness cost to position 160 amino acid substitutions. These data further define the wide range of activities that exist for PfCRT isoforms found in P. falciparum isolates from around the globe.

  8. PROMIS PF CAT Outperforms the ODI and SF-36 Physical Function Domain in Spine Patients.

    Science.gov (United States)

    Brodke, Darrel S; Goz, Vadim; Voss, Maren W; Lawrence, Brandon D; Spiker, William Ryan; Hung, Man

    2017-06-15

    The Oswestry Disability Index v2.0 (ODI), SF36 Physical Function Domain (SF-36 PFD), and PROMIS Physical Function CAT v1.2 (PF CAT) questionnaires were prospectively collected from 1607 patients complaining of back or leg pain, visiting a university-based spine clinic. All questionnaires were collected electronically, using a tablet computer. The aim of this study was to compare the psychometric properties of the PROMIS PF CAT with the ODI and SF36 Physical Function Domain in the same patient population. Evidence-based decision-making is improved by using high-quality patient-reported outcomes measures. Prior studies have revealed the shortcomings of the ODI and SF36, commonly used in spine patients. The PROMIS Network has developed measures with excellent psychometric properties. The Physical Function domain, delivered by Computerized Adaptive Testing (PF CAT), performs well in the spine patient population, though to-date direct comparisons with common measures have not been performed. Standard Rasch analysis was performed to directly compare the psychometrics of the PF CAT, ODI, and SF36 PFD. Spearman correlations were computed to examine the correlations of the three instruments. Time required for administration was also recorded. One thousand six hundred seven patients were administered all assessments. The time required to answer all items in the PF CAT, ODI, and SF-36 PFD was 44, 169, and 99 seconds. The ceiling and floor effects were excellent for the PF CAT (0.81%, 3.86%), while the ceiling effects were marginal and floor effects quite poor for the ODI (6.91% and 44.24%) and SF-36 PFD (5.97% and 23.65%). All instruments significantly correlated with each other. The PROMIS PF CAT outperforms the ODI and SF-36 PFD in the spine patient population and is highly correlated. It has better coverage, while taking less time to administer with fewer questions to answer. 2.

  9. Evaluation of the siRNA PF-04523655 versus ranibizumab for the treatment of neovascular age-related macular degeneration (MONET Study)

    DEFF Research Database (Denmark)

    Nguyen, Quan Dong; Schachar, Ronald A; Nduaka, Chudy I

    2012-01-01

    To evaluate the efficacy of different dosing paradigms of PF-04523655 (PF) versus ranibizumab (comparator) in subjects with neovascular age-related macular degeneration (AMD).......To evaluate the efficacy of different dosing paradigms of PF-04523655 (PF) versus ranibizumab (comparator) in subjects with neovascular age-related macular degeneration (AMD)....

  10. Contraceptive failure

    DEFF Research Database (Denmark)

    Rasch, Vibeke

    2002-01-01

    Most studies focusing on contraceptive failure in relation to pregnancy have focused on contraceptive failure among women having induced abortions, thereby neglecting those women who, despite contraceptive failure, accept the pregnancy and intend to carry the fetus to term. To get a more complete...... picture of the problem of contraceptive failure, this study focuses on contraceptive failure among women with diverse pregnancy outcomes. In all, 3520 pregnant women attending Odense University Hospital were included: 373 had induced abortions, 435 had spontaneous abortions, 97 had ectopic pregnancies......, and 2614 received antenatal care. The variables studied comprise age, partner relationship, number of births, occupational and economical situation, and contraceptive use.Contraceptive failure, defined as contraceptive use (condom, diaphragm, IUD, oral contraception, or another modern method...

  11. Heart Failure

    OpenAIRE

    McMurray, John; Ponikowski, Piotr

    2011-01-01

    Heart failure occurs in 3% to 4% of adults aged over 65 years, usually as a consequence of coronary artery disease or hypertension, and causes breathlessness, effort intolerance, fluid retention, and increased mortality. The 5-year mortality in people with systolic heart failure ranges from 25% to 75%, often owing to sudden death following ventricular arrhythmia. Risks of cardiovascular events are increased in people with left ventricular systolic dysfunction (LVSD) or heart failure.

  12. The synthetic Plasmodium falciparum circumsporozoite peptide PfCS102 as a malaria vaccine candidate: a randomized controlled phase I trial.

    Directory of Open Access Journals (Sweden)

    Régine Audran

    Full Text Available BACKGROUND: Fully efficient vaccines against malaria pre-erythrocytic stage are still lacking. The objective of this dose/adjuvant-finding study was to evaluate the safety, reactogenicity and immunogenicity of a vaccine candidate based on a peptide spanning the C-terminal region of Plasmodium falciparum circumsporozoite protein (PfCS102 in malaria naive adults. METHODOLOGY AND PRINCIPAL FINDINGS: Thirty-six healthy malaria-naive adults were randomly distributed into three dose blocks (10, 30 and 100 microg and vaccinated with PfCS102 in combination with either Montanide ISA 720 or GSK proprietary Adjuvant System AS02A at days 0, 60, and 180. Primary end-point (safety and reactogenicity was based on the frequency of adverse events (AE and of abnormal biological safety tests; secondary-end point (immunogenicity on P. falciparum specific cell-mediated immunity and antibody response before and after immunization. The two adjuvant formulations were well tolerated and their safety profile was good. Most AEs were local and, when systemic, involved mainly fatigue and headache. Half the volunteers in AS02A groups experienced severe AEs (mainly erythema. After the third injection, 34 of 35 volunteers developed anti-PfCS102 and anti-sporozoite antibodies, and 28 of 35 demonstrated T-cell proliferative responses and IFN-gamma production. Five of 22 HLA-A2 and HLA-A3 volunteers displayed PfCS102 specific IFN-gamma secreting CD8(+ T cell responses. Responses were only marginally boosted after the 3(rd vaccination and remained stable for 6 months. For both adjuvants, the dose of 10 microg was less immunogenic in comparison to 30 and 100 microg that induced similar responses. AS02A formulations with 30 microg or 100 microg PfCS102 induced about 10-folds higher antibody and IFN-gamma responses than Montanide formulations. CONCLUSIONS/SIGNIFICANCE: PfCS102 peptide was safe and highly immunogenic, allowing the design of more advanced trials to test its potential

  13. Heparin-independent, PF4-dependent binding of HIT antibodies to platelets: implications for HIT pathogenesis.

    Science.gov (United States)

    Padmanabhan, Anand; Jones, Curtis G; Bougie, Daniel W; Curtis, Brian R; McFarland, Janice G; Wang, Demin; Aster, Richard H

    2015-01-01

    Antibodies specific for platelet factor 4 (PF4)/heparin complexes are the hallmark of heparin-induced thrombocytopenia and thrombosis (HIT), but many antibody-positive patients have normal platelet counts. The basis for this is not fully understood, but it is believed that antibodies testing positive in the serotonin release assay (SRA) are the most likely to cause disease. We addressed this issue by characterizing PF4-dependent binding of HIT antibodies to intact platelets and found that most antibodies testing positive in the SRA, but none of those testing negative, bind to and activate platelets when PF4 is present without any requirement for heparin (P HIT antibodies recognize PF4 in a complex with heparin, only a subset of these antibodies recognize more subtle epitopes induced in PF4 when it binds to CS, the major platelet glycosaminoglycan. Antibodies having this property could explain "delayed HIT" seen in some individuals after discontinuation of heparin and the high risk for thrombosis that persists for weeks in patients recovered from HIT. © 2015 by The American Society of Hematology.

  14. Risk Factors, Clinical Presentation, and Outcomes in Overdose With Acetaminophen Alone or With Combination Products: Results From the Acute Liver Failure Study Group.

    Science.gov (United States)

    Serper, Marina; Wolf, Michael S; Parikh, Nikhil A; Tillman, Holly; Lee, William M; Ganger, Daniel R

    2016-01-01

    Acetaminophen (APAP) is the most common cause of acute liver failure (ALF) in the west. It is unknown if APAP overdose in combination with diphenhydramine or opioids confers a different clinical presentation or prognosis. Study objectives were to compare (1) baseline patient characteristics; (2) initial clinical presentation; and (3) clinical outcomes among patients with ALF due to APAP alone or in combination with diphenhydramine or opioids. We analyzed 666 cases of APAP-related liver failure using the Acute Liver Failure Study Group database from 1998 to 2012. The database contains detailed demographic, laboratory, and clinical outcome data, including hemodialysis, transplantation, and death and in-hospital complications such as arrhythmia and infection. The final sample included 666 patients with APAP liver injury. A total 30.3% of patients were overdosed with APAP alone, 14.1% with APAP/diphenhydramine, and 56.6% with APAP/opioids. Patients taking APAP with opioids were older, had more comorbidities, and were more likely to have unintentional overdose (all Ppresentation, 58% in the APAP/opioid group had advanced encephalopathy as compared with 43% with APAP alone (P=0.001) The APAP/diphenhydramine group presented with the highest serum aminotransferase levels, no differences in laboratory values were noted at 3 days postenrollment. No significant differences were observed in clinical outcomes among the groups. Most patients with APAP-induced ALF were taking APAP combination products. There were significant differences in patient characteristics and clinical presentation based on the type of product ingested, however, there were no differences noted in delayed hepatotoxicity or clinical outcomes.

  15. Accuracy of PfHRP2 versus Pf-pLDH antigen detection by malaria rapid diagnostic tests in hospitalized children in a seasonal hyperendemic malaria transmission area in Burkina Faso.

    Science.gov (United States)

    Maltha, Jessica; Guiraud, Issa; Lompo, Palpouguini; Kaboré, Bérenger; Gillet, Philippe; Van Geet, Chris; Tinto, Halidou; Jacobs, Jan

    2014-01-13

    In most sub-Saharan African countries malaria rapid diagnostic tests (RDTs) are now used for the diagnosis of malaria. Most RDTs used detect Plasmodium falciparum histidine-rich protein-2 (PfHRP2), though P. falciparum-specific parasite lactate dehydrogenase (Pf-pLDH)-detecting RDTs may have advantages over PfHRP2-detecting RDTs. Only few data are available on the use of RDTs in severe illness and the present study compared Pf-pLDH to PfHRP2-detection. Hospitalized children aged one month to 14 years presenting with fever or severe illness were included over one year. Venous blood samples were drawn for malaria diagnosis (microscopy and RDT), culture and complete blood count. Leftovers were stored at -80 °C and used for additional RDT analysis and PCR. An RDT targeting both PfHRP2 and Pf-pLDH was performed on all samples for direct comparison of diagnostic accuracy with microscopy as reference method. PCR was performed to explore false-positive RDT results. In 376 of 694 (54.2%) included children, malaria was microscopically confirmed. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value were 100.0, 70.9, 69.4 and 100.0%, respectively for PfHRP2-detection and 98.7, 94.0, 91.6 and 99.1%, respectively for Pf-pLDH-detection. Specificity and PPV were significantly lower for PfHRP2-detection (p <0.001). For both detection antigens, specificity was lowest for children one to five years and in the rainy season. PPV for both antigens was highest in the rainy season, because of higher malaria prevalence. False positive PfHRP2 results were associated with prior anti-malarial treatment and positive PCR results (98/114 (86.0%) samples tested). Among children presenting with severe febrile illness in a seasonal hyperendemic malaria transmission area, the present study observed similar sensitivity but lower specificity and PPV of PfHRP2 compared to Pf-pLDH-detection. Further studies should assess the diagnostic accuracy and safety of an

  16. Influence of matrix nature on the functional efficacy of biomedical cell product for the regeneration of damaged liver (experimental model of acute liver failure

    Directory of Open Access Journals (Sweden)

    S. V. Gautier

    2017-01-01

    Full Text Available Aim. A comparative analysis of the functional efficacy of biomedical cell products (BMCP for the regeneration of damaged liver based on biopolymer scaffolded porous and hydrogel matrices was performed on the experimental model of acute liver failure. Materials and methods. Matrices allowed for clinical use were employed for BMCP in the form of a sponge made from biopolymer nanostructured composite material (BNCM based on a highly purified bacterial copolymers of poly (β-hydroxybutyrate-co-β-oxyvalerate and polyethylene glycol and a hydrogel matrix from biopolymer microheterogeneous collagen-containing hydrogel (BMCH. Cellular component of BMCP was represented by liver cells and multipotent mesenchymal bone marrow stem cells. The functional efficacy of BMCP for the regeneration of damaged liver was evaluated on the experimental model of acute liver failure in Wistar rats (n = 40 via biochemical, morphological, and immunohistochemical methods. Results. When BMCP was implanted to regenerate the damaged liver on the basis of the scaffolded BNCM or hydrogel BMCH matrices, the lethality in rats with acute liver failure was absent; while in control it was 66.6%. Restoration of the activity of cytolytic enzyme levels and protein-synthetic liver function began on day 9 after modeling acute liver failure, in contrast to the control group, where recovery occurred only by days 18–21. Both matrices maintained the viability and functional activity of liver cells up to 90 days with the formation of blood vessels in BMCP. The obtained data confirm that scaffolded BNCM matrix and hydrogel BMCH matrix retain for a long time (up to 90 days the vital activity of the adherent cells in the BMCP composition, which allows using them to correct acute liver failure. At the same time, hydrogel matrix due to the presence of bioactive components contributes to the creation of the best conditions for adhesion and cell activity which accelerate the regeneration processes

  17. Enhancing blockade of Plasmodium falciparum erythrocyte invasion: assessing combinations of antibodies against PfRH5 and other merozoite antigens.

    Directory of Open Access Journals (Sweden)

    Andrew R Williams

    Full Text Available No vaccine has yet proven effective against the blood-stages of Plasmodium falciparum, which cause the symptoms and severe manifestations of malaria. We recently found that PfRH5, a P. falciparum-specific protein expressed in merozoites, is efficiently targeted by broadly-neutralizing, vaccine-induced antibodies. Here we show that antibodies against PfRH5 efficiently inhibit the in vitro growth of short-term-adapted parasite isolates from Cambodia, and that the EC(50 values of antigen-specific antibodies against PfRH5 are lower than those against PfAMA1. Since antibody responses elicited by multiple antigens are speculated to improve the efficacy of blood-stage vaccines, we conducted detailed assessments of parasite growth inhibition by antibodies against PfRH5 in combination with antibodies against seven other merozoite antigens. We found that antibodies against PfRH5 act synergistically with antibodies against certain other merozoite antigens, most notably with antibodies against other erythrocyte-binding antigens such as PfRH4, to inhibit the growth of a homologous P. falciparum clone. A combination of antibodies against PfRH4 and basigin, the erythrocyte receptor for PfRH5, also potently inhibited parasite growth. This methodology provides the first quantitative evidence that polyclonal vaccine-induced antibodies can act synergistically against P. falciparum antigens and should help to guide the rational development of future multi-antigen vaccines.

  18. Pregnancy-associated purpura fulminans (PF Púrpura fulminans asociada al embarazo

    Directory of Open Access Journals (Sweden)

    Gisela de la Rosa Echavez

    2005-03-01

    Full Text Available

    PF is a serious cutaneous purpuric disease, associated with an intravascular disseminated coagulation syndrome. Among its known causes, besides those of thrombophilia, are viral and bacterial infections, mostly Neisseria meningitidis. During pregnancy there are alterations in the hemostatic mechanism. However, by themselves, they have not been shown to lead to PF. The case of a 22-year-old woman who developed PF during pregnancy, presumably due to her Escherichia coli urinary tract infection, is presented including clinical, paraclinical, therapeutic and surgical aspects. She recovered but suffered amputation of the distant phalanges of three toes in the left foot 

    La púrpura fulminans (pf es una enfermedad cutánea purpúrica aguda asociada a un síndrome de coagulación intravascular diseminada (CID. Sus causas conocidas incluyen infecciones virales y bacterianas así como trombofilias. Es sabido que durante el embarazo existen alteraciones en los mecanismos hemostáticos, sin embargo, no se ha demostrado que estos fenómenos por sí solos ayuden al desarrollo de la PF. Se describe el caso de una mujer de 22 años, quien tuvo PF en circunstancias inusuales tales como el desarrollo de su cuadro durante el embarazo y el origen probable en una infección por E. coli. Se presentan los hallazgos clínicos, las intervenciones médicas y quirúrgicas y el desenlace. La paciente sobrevivió, pero hubo necesidad de amputarle las falanges distales de tres artejos del pie izquierdo.

  19. The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1 and Bacillus subtilis (UB_Bs1 on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae

    Directory of Open Access Journals (Sweden)

    Presti Mardiyani Purwaningtyas

    2016-11-01

    Full Text Available Parasitic Root-Lession nematode of Pratylenchus coffeae can reduce the Indonesian coffee plants productivity. Several studies reported that Pseudomonas fluorescens and Bacillus subtilis endophytic bacteria were antagonistic bacteria to nematode. The objective of this research was to reveal the effectiveness of bacterial colonies density of P. fluorescens (UB_Pf1, B.subtilis (UB BS1, and a combination of both bacteria on nematode mortality using median lethal concentration (LC50 and median lethal time 50 (LT50. The densities of bacteria used in this study were 107, 109, 1011 and 1013 cfu/ml. 35 testing nematodes were used and the mortality was counted at 6, 12, 24, 36, and 48 hours after treatments. The results showed that LC50 values of P. fluorescens was (UB_Pf1 was 4,3x108 cfu/ml, LC50 B. subtilis (UB_Bs1 was 1,9x109cfu/ ml, and LC50 combination of both bacteria was, 8x107 cfu/ml. It implies that the application of the combination of both bacteria are more pathogenic than single bacterial treatment. The results also showed that the highest LT50 value was 13.21  hours combination of bacterial colonies with a density of 1013 cfu/ml and the lowest LT50 value was 52.00 hours on P. fluorescens (UB_Pf1 treatment with colonies density of 107 cfu/ml.How to CitePurwaningtyas, P. M., Rahardjo, B. T., & Tarno, H. (2016. The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1 and Bacillus subtilis (UB_Bs1 on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae. Biosaintifika: Journal of Biology & Biology Education, 8(3, 286-293. 

  20. From failure to value: economic valuation for a selected set of products and services from Mediterranean forests

    Directory of Open Access Journals (Sweden)

    Davide Pettenella

    2016-04-01

    Full Text Available Aim of study: the paper estimates the economic value of a selected range of forest products and services, i.e. roundwood, non-wood forest products (NWFPs, and carbon-related services.Area of study: the research covers 21 Mediterranean countries, distinguished into four sub-regions.Material and methods: data have been gathered from official statistical sources (e.g. FAOSTAT, scientific literature and technical reports. Different estimation approaches based on market-price have been used for different products/services.Main results: the estimated value ranges between €10,512 and €11,158 million (M. Wood products represent more than 85% of the total value. Within them, industrial timber is the most relevant component (65%. Figures for NWFPs are likely to be underestimated because data are available only for some products and countries. When using alternative estimates for pine nuts, pine resin and cork, figures show a €36.8-572 M increase. In geographical terms, the economic value of Mediterranean forests is highly concentrated: North-West Mediterranean countries account for 70%, and nearly 90% is in just four countries (France, Spain, Turkey and Italy.Research highlights: enhancing the offer of Mediterranean forest products and increasing their role in the rural economy could help to reduce the costs of forest protection: a well-structured forest economy ensuring stable flows of incomes can provide a fundamental set of public non-market services and social values to both local people and the whole community. Understanding the true value of natural resources, then, is an essential step for promoting their protection and sustainable use.Abbreviations: Bln: billion; CUM: cubic meter; EM: East Mediterranean; FAO: Food and Agriculture Organisation of the United Nations; FRA: Forest Resource Assessment; ha: hectare; M: million; NEM: North-East Mediterranean; NWFP: non-wood forest product; NWM: North-West Mediterranean; SM: South Mediterranean. 

  1. Pressure-induced phase transition of 1-butyl-3-methylimidazolium hexafluorophosphate [bmim][PF6

    Science.gov (United States)

    Takekiyo, Takahiro; Hatano, Naohiro; Imai, Yusuke; Abe, Hiroshi; Yoshimura, Yukihiro

    2011-03-01

    We have investigated the pressure-induced Raman spectral change of 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][PF6]) using Raman spectroscopy. The relative Raman intensity at 590 cm-1 of the CH2 rocking band assigned to the gauche conformer of the NCCC dihedral angle of the butyl group in the [bmim]+ cation increases when the pressure-induced liquid-crystalline phase transition occurs, while that at 610 cm-1 assigned to the trans conformer decreases. Our results show that the high-pressure phase transition of [bmim][PF6] causes the increase of the gauche conformer of the [bmim]+ cation.

  2. Analysis of the flow imbalance in the KSTAR PF cryo-circuit

    International Nuclear Information System (INIS)

    Lee, Hyun-Jung; Park, Dong-Seong; Kwag, Sang-Woo; Joo, Jae-Jun; Moon, Kyung-Mo; Kim, Nam-Won; Lee, Young-Joo; Park, Young-Min; Yang, Hyung-Lyeol

    2015-01-01

    Highlights: • Investigate of flow imbalance trend for the KSTAR PF superconducting magnet. • Flow imbalance is compared with individual magnet test and integration magnet test. • Intensifying of flow imbalance is proven from the flow monitoring in the KSTAR PF circuit. • Flow behavior is analyzed during magnet charging in the circulator circuit. • Variation of magnet outlet temperature is analyzed due to flow imbalance. - Abstract: The KSTAR PF cryo-circuit is a quasi-closed circulation system in which more than 370 g/s of supercritical helium (SHe) is circulated using a SHe circulator. The heated helium from superconducting magnet is cooled through sub cooler (4.3 K). The circulator is operated at 4.5 K and 6.5 bar, and the pressure drop of the circuit is kept at 2 bar in order to maintain the supercritical state and circulator stability. The circuit is connected with helium refrigerator system, distribution system, and supercritical magnet system. It has a hundred branches to supply supercritical helium to the poloidal field superconducting magnet. The branch was designed to optimize the operation conditions and they are grouped for one cryogenic valve has the same length within the cardinal principle of the optimization. Five cryogenic valves are installed to control the mass flow rate, and seven orifice mass flow meters, differential pressure gauges and temperature sensors were installed in front of the magnet in the distribution because upper magnet and lower magnet is symmetric theoretically. The cryogenic pipe line was manufactured with elevation about 10 m between upper magnet and lower magnet. The inlet and outlet helium feed-through were installed at the coil inside in case of KSTAR PF1–PF5 upper magnet and lower magnet. The flow imbalance is caused by void fraction and it could be changed due to manufacturing process even if it has the same length of cooling channel. This creates an imbalance among cooling channels and temperatures are

  3. Initial characterization of the Pf-Int recombinase from the malaria parasite Plasmodium falciparum.

    Directory of Open Access Journals (Sweden)

    Mehdi Ghorbal

    Full Text Available Genetic variation is an essential means of evolution and adaptation in many organisms in response to environmental change. Certain DNA alterations can be carried out by site-specific recombinases (SSRs that fall into two families: the serine and the tyrosine recombinases. SSRs are seldom found in eukaryotes. A gene homologous to a tyrosine site-specific recombinase has been identified in the genome of Plasmodium falciparum. The sequence is highly conserved among five other members of Plasmodia.The predicted open reading frame encodes for a ∼57 kDa protein containing a C-terminal domain including the putative tyrosine recombinase conserved active site residues R-H-R-(H/W-Y. The N-terminus has the typical alpha-helical bundle and potentially a mixed alpha-beta domain resembling that of λ-Int. Pf-Int mRNA is expressed differentially during the P. falciparum erythrocytic life stages, peaking in the schizont stage. Recombinant Pf-Int and affinity chromatography of DNA from genomic or synthetic origin were used to identify potential DNA targets after sequencing or micro-array hybridization. Interestingly, the sequences captured also included highly variable subtelomeric genes such as var, rif, and stevor sequences. Electrophoretic mobility shift assays with DNA were carried out to verify Pf-Int/DNA binding. Finally, Pf-Int knock-out parasites were created in order to investigate the biological role of Pf-Int.Our data identify for the first time a malaria parasite gene with structural and functional features of recombinases. Pf-Int may bind to and alter DNA, either in a sequence specific or in a non-specific fashion, and may contribute to programmed or random DNA rearrangements. Pf-Int is the first molecular player identified with a potential role in genome plasticity in this pathogen. Finally, Pf-Int knock-out parasite is viable showing no detectable impact on blood stage development, which is compatible with such function.

  4. Systems concepts for DOE facilities: analysis of PF/LASS data

    International Nuclear Information System (INIS)

    Bearse, R.C.; Shirk, D.G.; Marshall, R.S.; Thomas, C.C. Jr.

    1982-06-01

    We have analyzed Plutonium Facility/Los Alamos Safeguards System (PF/LASS) data for the Fast Flux Test Facility (FFTF) process. Highlights of the work are: the PF/LASS data base provides useful information for accountability purposes, some measurement code assignments appear to be in error, some other data are erroneous, and material in process (MIP) and cumulative sum (CUSUM) charts are powerful indicators of trouble areas. From these studies we recommend re-examination of instrument biases, adoption of new naming procedures for collection batches, improvement of measurement code assignment reliability, revision of round-off procedures, and strengthening of measurement control procedures

  5. From failure to value: economic valuation for a selected set of products and services from Mediterranean forests

    Energy Technology Data Exchange (ETDEWEB)

    Masiero, M.; Pettenella, D.; Secco, L.

    2016-07-01

    Aim of study: the paper estimates the economic value of a selected range of forest products and services, i.e. roundwood, non-wood forest products (NWFPs), and carbon-related services. Area of study: the research covers 21 Mediterranean countries, distinguished into four sub-regions. Material and methods: data have been gathered from official statistical sources (e.g. FAOSTAT), scientific literature and technical reports. Different estimation approaches based on market-price have been used for different products/services. Main results: the estimated value ranges between €10,512 and €11,158 million (M). Wood products represent more than 85% of the total value. Within them, industrial timber is the most relevant component (65%). Figures for NWFPs are likely to be underestimated because data are available only for some products and countries. When using alternative estimates for pine nuts, pine resin and cork, figures show a €36.8-572 M increase. In geographical terms, the economic value of Mediterranean forests is highly concentrated: North-West Mediterranean countries account for 70%, and nearly 90% is in just four countries (France, Spain, Turkey and Italy). Research highlights: enhancing the offer of Mediterranean forest products and increasing their role in the rural economy could help to reduce the costs of forest protection: a well-structured forest economy ensuring stable flows of incomes can provide a fundamental set of public non-market services and social values to both local people and the whole community. Understanding the true value of natural resources, then, is an essential step for promoting their protection and sustainable use. (Author)

  6. Mixed Acid-Base Disorders, Hydroelectrolyte Imbalance and Lactate Production in Hypercapnic Respiratory Failure: The Role of Noninvasive Ventilation

    Science.gov (United States)

    Terzano, Claudio; Di Stefano, Fabio; Conti, Vittoria; Di Nicola, Marta; Paone, Gregorino; Petroianni, Angelo; Ricci, Alberto

    2012-01-01

    Background Hypercapnic Chronic Obstructive Pulmonary Disease (COPD) exacerbation in patients with comorbidities and multidrug therapy is complicated by mixed acid-base, hydro-electrolyte and lactate disorders. Aim of this study was to determine the relationships of these disorders with the requirement for and duration of noninvasive ventilation (NIV) when treating hypercapnic respiratory failure. Methods Sixty-seven consecutive patients who were hospitalized for hypercapnic COPD exacerbation had their clinical condition, respiratory function, blood chemistry, arterial blood gases, blood lactate and volemic state assessed. Heart and respiratory rates, pH, PaO2 and PaCO2 and blood lactate were checked at the 1st, 2nd, 6th and 24th hours after starting NIV. Results Nine patients were transferred to the intensive care unit. NIV was performed in 11/17 (64.7%) mixed respiratory acidosis–metabolic alkalosis, 10/36 (27.8%) respiratory acidosis and 3/5 (60%) mixed respiratory-metabolic acidosis patients (p = 0.026), with durations of 45.1±9.8, 36.2±8.9 and 53.3±4.1 hours, respectively (p = 0.016). The duration of ventilation was associated with higher blood lactate (prespiratory acidosis patients. Hypovolemic hyponatremia with hypochloremia and hypokalemia occurred in 10 mixed respiratory acidosis–metabolic alkalosis patients, and euvolemic hypochloremia occurred in the other 7 patients with this mixed acid-base disorder. Conclusions Mixed acid-base and lactate disorders during hypercapnic COPD exacerbations predict the need for and longer duration of NIV. The combination of mixed acid-base disorders and hydro-electrolyte disturbances should be further investigated. PMID:22539963

  7. Frequency and sources of basin floor turbidites in alfonso basin, Gulf of California, Mexico: Products of slope failures

    Science.gov (United States)

    Gonzalez-Yajimovich, Oscar E.; Gorsline, Donn S.; Douglas, Robert G.

    2007-07-01

    Alfonso Basin is a small margin basin formed by extensional tectonics in the actively rifting, seismically active Gulf of California. The basin is centered at 24°40' N and 110° 38' W, and is a closed depression (maximum depth 420 m) with an effective sill depth of about 320 m (deepest sill), a width of 20 km and length of 25 km. Basin floor area below a depth of 350 m is about 260 km 2. The climate is arid to semiarid but was wetter during the early (ca. 10,000-7000 Calendar years Before Present [BP]) and middle Holocene (ca. 7000-4000 Cal. Years BP). Basin-wide turbidity currents reach the floor of Alfonso Basin at centennial to millennial intervals. The peninsular drainages tributary to the basin are small and have maximum flood discharges of the order of 10 4m 3. The basin-floor turbidites thicker than 1 cm have volumes of the order of 10 6m 3 to 10 8m 3 and require a much larger source. The largest turbidite seen in our cores is ca. 1 m thick in the central basin floor and was deposited 4900 Calendar Years Before Present (BP). Two smaller major events occurred about 1500 and 2800 Cal. Years BP. Seismicity over the past century of record shows a clustering of larger epicenters along faults forming the eastern Gulf side of Alfonso Basin. In that period there have been four earthquakes with magnitudes above 7.0 but all are distant from the basin. Frequency of such earthquakes in the basin vicinity is probably millennial. It is concluded that the basin-wide turbidites thicker than 1 cm must be generated by slope failures on the eastern side of the basin at roughly millennial intervals. The thin flood turbidites have a peninsular source at centennial frequencies.

  8. Mixed acid-base disorders, hydroelectrolyte imbalance and lactate production in hypercapnic respiratory failure: the role of noninvasive ventilation.

    Directory of Open Access Journals (Sweden)

    Claudio Terzano

    Full Text Available BACKGROUND: Hypercapnic Chronic Obstructive Pulmonary Disease (COPD exacerbation in patients with comorbidities and multidrug therapy is complicated by mixed acid-base, hydro-electrolyte and lactate disorders. Aim of this study was to determine the relationships of these disorders with the requirement for and duration of noninvasive ventilation (NIV when treating hypercapnic respiratory failure. METHODS: Sixty-seven consecutive patients who were hospitalized for hypercapnic COPD exacerbation had their clinical condition, respiratory function, blood chemistry, arterial blood gases, blood lactate and volemic state assessed. Heart and respiratory rates, pH, PaO(2 and PaCO(2 and blood lactate were checked at the 1st, 2nd, 6th and 24th hours after starting NIV. RESULTS: Nine patients were transferred to the intensive care unit. NIV was performed in 11/17 (64.7% mixed respiratory acidosis-metabolic alkalosis, 10/36 (27.8% respiratory acidosis and 3/5 (60% mixed respiratory-metabolic acidosis patients (p = 0.026, with durations of 45.1 ± 9.8, 36.2 ± 8.9 and 53.3 ± 4.1 hours, respectively (p = 0.016. The duration of ventilation was associated with higher blood lactate (p<0.001, lower pH (p = 0.016, lower serum sodium (p = 0.014 and lower chloride (p = 0.038. Hyponatremia without hypervolemic hypochloremia occurred in 11 respiratory acidosis patients. Hypovolemic hyponatremia with hypochloremia and hypokalemia occurred in 10 mixed respiratory acidosis-metabolic alkalosis patients, and euvolemic hypochloremia occurred in the other 7 patients with this mixed acid-base disorder. CONCLUSIONS: Mixed acid-base and lactate disorders during hypercapnic COPD exacerbations predict the need for and longer duration of NIV. The combination of mixed acid-base disorders and hydro-electrolyte disturbances should be further investigated.

  9. Mixed acid-base disorders, hydroelectrolyte imbalance and lactate production in hypercapnic respiratory failure: the role of noninvasive ventilation.

    Science.gov (United States)

    Terzano, Claudio; Di Stefano, Fabio; Conti, Vittoria; Di Nicola, Marta; Paone, Gregorino; Petroianni, Angelo; Ricci, Alberto

    2012-01-01

    Hypercapnic Chronic Obstructive Pulmonary Disease (COPD) exacerbation in patients with comorbidities and multidrug therapy is complicated by mixed acid-base, hydro-electrolyte and lactate disorders. Aim of this study was to determine the relationships of these disorders with the requirement for and duration of noninvasive ventilation (NIV) when treating hypercapnic respiratory failure. Sixty-seven consecutive patients who were hospitalized for hypercapnic COPD exacerbation had their clinical condition, respiratory function, blood chemistry, arterial blood gases, blood lactate and volemic state assessed. Heart and respiratory rates, pH, PaO(2) and PaCO(2) and blood lactate were checked at the 1st, 2nd, 6th and 24th hours after starting NIV. Nine patients were transferred to the intensive care unit. NIV was performed in 11/17 (64.7%) mixed respiratory acidosis-metabolic alkalosis, 10/36 (27.8%) respiratory acidosis and 3/5 (60%) mixed respiratory-metabolic acidosis patients (p = 0.026), with durations of 45.1 ± 9.8, 36.2 ± 8.9 and 53.3 ± 4.1 hours, respectively (p = 0.016). The duration of ventilation was associated with higher blood lactate (prespiratory acidosis patients. Hypovolemic hyponatremia with hypochloremia and hypokalemia occurred in 10 mixed respiratory acidosis-metabolic alkalosis patients, and euvolemic hypochloremia occurred in the other 7 patients with this mixed acid-base disorder. Mixed acid-base and lactate disorders during hypercapnic COPD exacerbations predict the need for and longer duration of NIV. The combination of mixed acid-base disorders and hydro-electrolyte disturbances should be further investigated.

  10. Plasmodium falciparum Hep1 Is Required to Prevent the Self Aggregation of PfHsp70-3.

    Directory of Open Access Journals (Sweden)

    David O Nyakundi

    Full Text Available The majority of mitochondrial proteins are encoded in the nucleus and need to be imported from the cytosol into the mitochondria, and molecular chaperones play a key role in the efficient translocation and proper folding of these proteins in the matrix. One such molecular chaperone is the eukaryotic mitochondrial heat shock protein 70 (Hsp70; however, it is prone to self-aggregation and requires the presence of an essential zinc-finger protein, Hsp70-escort protein 1 (Hep1, to maintain its structure and function. PfHsp70-3, the only Hsp70 predicted to localize in the mitochondria of P. falciparum, may also rely on a Hep1 orthologue to prevent self-aggregation. In this study, we identified a putative Hep1 orthologue in P. falciparum and co-expression of PfHsp70-3 and PfHep1 enhanced the solubility of PfHsp70-3. PfHep1 suppressed the thermally induced aggregation of PfHsp70-3 but not the aggregation of malate dehydrogenase or citrate synthase, thus showing specificity for PfHsp70-3. Zinc ions were indeed essential for maintaining the function of PfHep1, as EDTA chelation abrogated its abilities to suppress the aggregation of PfHsp70-3. Soluble and functional PfHsp70-3, acquired by co-expression with PfHep-1, will facilitate the biochemical characterisation of this particular Hsp70 protein and its evaluation as a drug target for the treatment of malaria.

  11. Identification of active Plasmodium falciparum calpain to establish screening system for Pf-calpain-based drug development

    Directory of Open Access Journals (Sweden)

    Soh Byoung

    2013-02-01

    Full Text Available Abstract Background With the increasing resistance of malaria parasites to available drugs, there is an urgent demand to develop new anti-malarial drugs. Calpain inhibitor, ALLN, is proposed to inhibit parasite proliferation by suppressing haemoglobin degradation. This provides Plasmodium calpain as a potential target for drug development. Pf-calpain, a cysteine protease of Plasmodium falciparum, belongs to calpain-7 family, which is an atypical calpain not harboring Ca2+-binding regulatory motifs. In this present study, in order to establish the screening system for Pf-calpain specific inhibitors, the active form of Pf-calpain was first identified. Methods Recombinant Pf-calpain including catalytic subdomain IIa (rPfcal-IIa was heterologously expressed and purified. Enzymatic activity was determined by both fluorogenic substrate assay and gelatin zymography. Molecular homology modeling was carried out to address the activation mode of Pf-calpain in the aspect of structural moiety. Results Based on the measurement of enzymatic activity and protease inhibitor assay, it was found that the active form of Pf-calpain only contains the catalytic subdomain IIa, suggesting that Pf-calpain may function as a monomeric form. The sequence prediction indicates that the catalytic subdomain IIa contains all amino acid residues necessary for catalytic triad (Cys-His-Asn formation. Molecular modeling suggests that the Pf-calpain subdomain IIa makes an active site, holding the catalytic triad residues in their appropriate orientation for catalysis. The mutation analysis further supports that those amino acid residues are functional and have enzymatic activity. Conclusion The identified active form of Pf-calpain could be utilized to establish high-throughput screening system for Pf-calpain inhibitors. Due to its unique monomeric structural property, Pf-calpain could be served as a novel anti-malarial drug target, which has a high specificity for malaria parasite

  12. Heart Failure

    Science.gov (United States)

    ... Other diseases. Chronic diseases — such as diabetes, HIV, hyperthyroidism, hypothyroidism, or a buildup of iron (hemochromatosis) or ... transplantation or support with a ventricular assist device. Prevention The key to preventing heart failure is to ...

  13. Application of ISO22000, failure mode, and effect analysis (FMEA) cause and effect diagrams and pareto in conjunction with HACCP and risk assessment for processing of pastry products.

    Science.gov (United States)

    Varzakas, Theodoros H

    2011-09-01

    The Failure Mode and Effect Analysis (FMEA) model has been applied for the risk assessment of pastry processing. A tentative approach of FMEA application to the pastry industry was attempted in conjunction with ISO22000. Preliminary Hazard Analysis was used to analyze and predict the occurring failure modes in a food chain system (pastry processing plant), based on the functions, characteristics, and/or interactions of the ingredients or the processes, upon which the system depends. Critical Control points have been identified and implemented in the cause and effect diagram (also known as Ishikawa, tree diagram, and fishbone diagram). In this work a comparison of ISO22000 analysis with HACCP is carried out over pastry processing and packaging. However, the main emphasis was put on the quantification of risk assessment by determining the Risk Priority Number (RPN) per identified processing hazard. Storage of raw materials and storage of final products at -18°C followed by freezing were the processes identified as the ones with the highest RPN (225, 225, and 144 respectively) and corrective actions were undertaken. Following the application of corrective actions, a second calculation of RPN values was carried out leading to considerably lower values (below the upper acceptable limit of 130). It is noteworthy that the application of Ishikawa (Cause and Effect or Tree diagram) led to converging results thus corroborating the validity of conclusions derived from risk assessment and FMEA. Therefore, the incorporation of FMEA analysis within the ISO22000 system of a pastry processing industry is considered imperative.

  14. Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells

    Directory of Open Access Journals (Sweden)

    Yu Ri Kim

    2013-01-01

    Full Text Available High doses of acetaminophen (APAP; N-acetyl-p-aminophenol cause severe hepatotoxicity after metabolic activation by cytochrome P450 2E1. This study was undertaken to examine the preventive effects of thiacremonone, a compound extracted from garlic, on APAP-induced acute hepatic failure in male C57BL/6J. Mice received with 500 mg/kg APAP after a 7-day pretreatment with thiacremonone (10–50 mg/kg. Thiacremonone inhibited the APAP-induced serum ALT and AST levels in a dose-dependent manner, and markedly reduced the restricted area of necrosis and inflammation by administration of APAP. Thiacremonone also inhibited the APAP-induced depletion of intracellular GSH, induction of nitric oxide, and lipid peroxidation as well as expression of P450 2E1. After APAP injection, the numbers of Kupffer cells, natural killer cells, and cytotoxic T cells were elevated, but the elevated cell numbers in the liver were reduced in thiacremonone pretreated mice. The expression levels of I-309, M-CSF, MIG, MIP-1α, MIP-1β, IL-7, and IL-17 were increased by APAP treatment, which were inhibited in thiacremonone pretreated mice. These data indicate that thiacremonone could be a useful agent for the treatment of drug-induced hepatic failure and that the reduction of cytotoxic immune cells as well as proinflammatory cytokine production may be critical for the prevention of APAP-induced acute liver toxicity.

  15. Calculation of fuel pin failure timing under LOCA conditions

    International Nuclear Information System (INIS)

    Jones, K.R.; Wade, N.L.; Siefken, L.J.; Straka, M.; Katsma, K.R.

    1991-10-01

    The objective of this research was to develop and demonstrate a methodology for calculation of the time interval between receipt of the containment isolation signals and the first fuel pin failure for loss-of-coolant accidents (LOCAs). Demonstration calculations were performed for a Babcock and Wilcox (B ampersand W) design (Oconee) and a Westinghouse (W) 4-loop design (Seabrook). Sensitivity studies were performed to assess the impacts of fuel pin burnup, axial peaking factor, break size, emergency core cooling system (ECCS) availability, and main coolant pump trip on these items. The analysis was performed using a four-code approach, comprised of FRAPCON-2, SCDAP/RELAP5/MOD3, TRAC-PF1/MOD1, and FRAP-T6. In addition to the calculation of timing results, this analysis provided a comparison of the capabilities of SCDAP/RELAP5/MOD3 with TRAC-PF1/MOD1 for large-break LOCA analysis. This paper discusses the methodology employed and the code development efforts required to implement the methodology. The shortest time intervals calculated between initiation of containment isolation and fuel pin failure were 11.4 s and 19.1 for the B ampersand W and W plants, respectively. The FRAP-T6 fuel pin failure times calculated using thermal-hydraulic data generated by SCDAP/RELAP5/MOD3 were more conservative than those calculated using data generated by TRAC-PF1/MOD1. 18 refs., 7 figs., 4 tabs

  16. Acellular therapeutic approach for heart failure: in vitro production of extracellular vesicles from human cardiovascular progenitors.

    Science.gov (United States)

    El Harane, Nadia; Kervadec, Anaïs; Bellamy, Valérie; Pidial, Laetitia; Neametalla, Hany J; Perier, Marie-Cécile; Lima Correa, Bruna; Thiébault, Léa; Cagnard, Nicolas; Duché, Angéline; Brunaud, Camille; Lemitre, Mathilde; Gauthier, Jeanne; Bourdillon, Alexandra T; Renault, Marc P; Hovhannisyan, Yeranuhi; Paiva, Solenne; Colas, Alexandre R; Agbulut, Onnik; Hagège, Albert; Silvestre, Jean-Sébastien; Menasché, Philippe; Renault, Nisa K E

    2018-05-21

    We have shown that extracellular vesicles (EVs) secreted by embryonic stem cell-derived cardiovascular progenitor cells (Pg) recapitulate the therapeutic effects of their parent cells in a mouse model of chronic heart failure (CHF). Our objectives are to investigate whether EV released by more readily available cell sources are therapeutic, whether their effectiveness is influenced by the differentiation state of the secreting cell, and through which mechanisms they act. The total EV secreted by human induced pluripotent stem cell-derived cardiovascular progenitors (iPSC-Pg) and human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) were isolated by ultracentrifugation and characterized by Nanoparticle Tracking Analysis, western blot, and cryo-electron microscopy. In vitro bioactivity assays were used to evaluate their cellular effects. Cell and EV microRNA (miRNA) content were assessed by miRNA array. Myocardial infarction was induced in 199 nude mice. Three weeks later, mice with left ventricular ejection fraction (LVEF) ≤ 45% received transcutaneous echo-guided injections of iPSC-CM (1.4 × 106, n = 19), iPSC-Pg (1.4 × 106, n = 17), total EV secreted by 1.4 × 106 iPSC-Pg (n = 19), or phosphate-buffered saline (control, n = 17) into the peri-infarct myocardium. Seven weeks later, hearts were evaluated by echocardiography, histology, and gene expression profiling, blinded to treatment group. In vitro, EV were internalized by target cells, increased cell survival, cell proliferation, and endothelial cell migration in a dose-dependent manner and stimulated tube formation. Extracellular vesicles were rich in miRNAs and most of the 16 highly abundant, evolutionarily conserved miRNAs are associated with tissue-repair pathways. In vivo, EV outperformed cell injections, significantly improving cardiac function through decreased left ventricular volumes (left ventricular end systolic volume: -11%, P < 0.001; left

  17. Bridge joint fabrication and validation for SST-1 PF coil winding pack

    International Nuclear Information System (INIS)

    Prasad, Upendra; Sharma, A.N.; Patel, D.; Doshi, K.; Varmora, P.; Khristi, Y.; Pradhan, S.

    2014-01-01

    Highlights: • Prototype of bridge type joints fabricated and validated successfully. • Bridge type joints fabricated and validated on one of the SST-1 PF#3T coil successfully. • Joint resistance was measured with precision nano volt meter and PXI based data acquisition system. • Leak tightness of joint box was better than 3 × 10 −6 Pa m 3 s −1 . • The measured joint resistance of bridge type joint was ∼1.6 nano ohm. - Abstract: A novel concept of bridge joint for Poloidal field (PF) magnet of SST-1 with damaged winding pack has been realized. This joint has been fabricated on 5th and 6th layers of PF#3T coil winding pack (WP) after validation at 10 kA at liquid helium temperature of 4.2 K in current lead test chamber. The joint resistance of bridge joint was measured ∼1.6 nΩ at flat top DC current of 10 kA. This type of joint could be economically useful for revival of a shorted and damaged WP superconducting PF magnets of Tokamaks. In this paper, details of bridge joint design, fabrication and validations are discussed

  18. Hall effect in the normal phase of the organic superconductor (TMTSF)2PF6

    DEFF Research Database (Denmark)

    Moser, J.; Cooper, J.R.; Jerome, D.

    2000-01-01

    We report accurate Hall effect measurements performed in the normal phase of the quasi-one-dimensional organic conductor (TMTSF)(2)PF(6) at ambient pressure. The Hall coefficient is found to be strongly temperature dependent all the way from 300 K down to the spin density wave onset arising aroun...

  19. Dimensionality Crossover in the Organic Superconductor Tetramethyltetraselenafulvalene Hexafluorophosphate [(TMTSF)2PF6

    DEFF Research Database (Denmark)

    Jacobsen, Claus Schelde; Tanner, D. B.; Bechgaard, K.

    1981-01-01

    Polarized reflectance measurements from the far infrared to the visible are reported for (TMTSF)2PF6. At low temperatures, both parallel and perpendicular polarizations show plasma edges in the infrared with metallic reflection below these edges, indicating two- or three-dimensional behavior. The...

  20. Fridel-Crafts acylation using bismuth triflate in [BMI][PF6

    DEFF Research Database (Denmark)

    Tran, Phuong Hoang; Duus, Fritz; Le, Thach Ngoc

    2012-01-01

    Bismuth trifluoromethanesulfonate was found to be a good catalyst for the Friedel–Craftsacylation. Bismuthtriflate immobilized in an ionic liquid was the most efficient catalytic system. Bismuthtriflate in [BMI][PF6] catalyzes this reaction under microwave irradiation allowing the rapid synthesis...

  1. Impacts of P-f & Q-V Droop Control on MicroGrids Transient Stability

    Science.gov (United States)

    Zhao-xia, Xiao; Hong-wei, Fang

    Impacts of P-f & Q-V droop control on MicroGrid transient stability was investigated with a wind unit of asynchronous generator in the MicroGrid. The system frequency stability was explored when the motor load starts and its load power changes, and faults of different types and different locations occurs. The simulations were done by PSCAD/EMTDC.

  2. Design of coolant distribution system (CDS) for ITER PF AC/DC converter

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Bin [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Song, Zhiquan, E-mail: zhquansong@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Fu, Peng; Xu, Xuesong; Li, Chuan [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Wang, Min; Dong, Lin [China International Nuclear Fusion Energy Program Execution Center, Beijing 100862 (China)

    2016-10-15

    Highlights: • System process and arrangement has been proposed to meet the multiple requirements from the converter system. • Thermal hydraulic analysis model has been developed to size and predict the system operation behavior. • Prototype test has been performed to validate the proposed design methodology. - Abstract: The Poloidal Field (PF) converter unit, playing an essential role in the plasma shape and position control in vertical and horizontal direction, which is an important part of ITER power supply system. As an important subsystem of the converter unit, the coolant distribution system has the function to distribute the cooling water from ITER component cooling water system (CCWS) to its main components at the required flow rate, pressure and temperature. This paper presents the thermal hydraulic design of coolant distribution system for the ITER PF converter unit. Different operational requirements of the PF converter unit regarding flow rate, temperature and pressure have been analyzed to design the system process and arrangement. A thermal-hydraulic analysis model has been built to size the system and predict the flow rate and temperature distribution of the system under the normal operation. Based on the system thermal-hydraulic analysis results, the system pressure profile has been plotted to evaluate the pressure behavior along each client flow path. A CDS prototype for the ITER PF converter has been constructed and some experiments have been performed on it. A good agreement of the flow distribution and temperature behavior between the simulated and test results validate the proposed design methodology.

  3. Beam transport of PF (Positron Factory) 2.5-GeV linac

    International Nuclear Information System (INIS)

    Shiraga, Takahiro; Asami, Akira; Suwada, Tsuyoshi; Kobayashi, Hitoshi.

    1993-01-01

    The beam transport is one of the most important problems in the linac to be used as the injector for the B-FACTORY accelerators. A basic problem of the beam transport is how to correct transport parameters immediately when a klystron becomes off. This is studied with the PF (Positron Factory) 2.5-GeV linac. (author)

  4. Performance of CareStart™ Malaria Pf/Pv Combo test for the ...

    African Journals Online (AJOL)

    Background: CareStart™ Malaria Pf/Pv Combo test has shown encouraging results for the diagnosis of P. falciparum and P. vivax infections in Ethiopia. Nevertheless, the performance of the test could be affected by different factors like gene polymorphisms, excess heat and humidity. Hence, evaluation of the performance of ...

  5. Characterization of the integrated filamentous phage Pf5 and its involvement in small-colony formation

    NARCIS (Netherlands)

    Mooij, Marlies J.; Drenkard, Eliana; Llamas, María A.; Vandenbroucke-Grauls, Christina M. J. E.; Savelkoul, Paul H. M.; Ausubel, Frederick M.; Bitter, Wilbert

    2007-01-01

    Bacteriophages play an important role in bacterial virulence and phenotypic variation. It has been shown that filamentous bacteriophage Pf4 of Pseudomonas aeruginosa strain PAO1 mediates the formation of small-colony variants (SCVs) in biofilms. This morphology type is associated with parameters of

  6. Multiscale Simulations of Lamellar PS–PEO Block Copolymers Doped with LiPF6 Ions

    KAUST Repository

    Sethuraman, Vaidyanathan; Mogurampelly, Santosh; Ganesan, Venkat

    2017-01-01

    We report the results of atomistic simulations of the structural equilibrium properties of PS–PEO block copolymer (BCP) melt in the ordered lamellar phase doped with LiPF6 salt. A hybrid simulation strategy, consisting of steps of coarse

  7. Iron is a substrate of the Plasmodium falciparum chloroquine resistance transporter PfCRT in Xenopus oocytes.

    Science.gov (United States)

    Bakouh, Naziha; Bellanca, Sebastiano; Nyboer, Britta; Moliner Cubel, Sonia; Karim, Zoubida; Sanchez, Cecilia P; Stein, Wilfred D; Planelles, Gabrielle; Lanzer, Michael

    2017-09-29

    The chloroquine resistance transporter of the human malaria parasite Plasmodium falciparum , PfCRT, is an important determinant of resistance to several quinoline and quinoline-like antimalarial drugs. PfCRT also plays an essential role in the physiology of the parasite during development inside erythrocytes. However, the function of this transporter besides its role in drug resistance is still unclear. Using electrophysiological and flux experiments conducted on PfCRT-expressing Xenopus laevis oocytes, we show here that both wild-type PfCRT and a PfCRT variant associated with chloroquine resistance transport both ferrous and ferric iron, albeit with different kinetics. In particular, we found that the ability to transport ferrous iron is reduced by the specific polymorphisms acquired by the PfCRT variant as a result of chloroquine selection. We further show that iron and chloroquine transport via PfCRT is electrogenic. If these findings in the Xenopus model extend to P. falciparum in vivo , our data suggest that PfCRT might play a role in iron homeostasis, which is essential for the parasite's development in erythrocytes. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. ION-EXCHANGE IMMUNOAFFINITY PURIFICATION OF A RECOMBINANT BACULOVIRUS PLASMODIUM-FALCIPARUM APICAL MEMBRANE ANTIGEN, PF83/AMA-1

    NARCIS (Netherlands)

    NARUM, DL; WELLING, GW; THOMAS, AW

    1993-01-01

    A two-step purification regime has been developed for a quantitatively minor, putatively transmembrane, M(r) 83 000, apical membrane blood stage vaccine candidate antigen of Plasmodium falciparum (PF83/AMA-1), that has been expressed as a full-length baculovirus recombinant protein, PF83-FG8-1. The

  9. Roles of Rhizoxin and 2,4-diacetylphloroglucinol in Suppression of Fusarium spp. by the Rhizobacterium Pseudomonas fluorescens Pf-5

    Science.gov (United States)

    Pseudomonas fluorescens strain Pf-5 is a rhizosphere bacterium that acts as a biocontrol agent of soilborne plant diseases and produces at least 10 different secondary metabolites, including several with antifungal properties. We derived site-directed mutants of Pf-5 with single and multiple mutatio...

  10. 49 CFR 178.358 - Specification 21PF fire and shock resistant, phenolic-foam insulated, metal overpack.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Specification 21PF fire and shock resistant, phenolic-foam insulated, metal overpack. 178.358 Section 178.358 Transportation Other Regulations Relating... Class 7 (Radioactive) Materials § 178.358 Specification 21PF fire and shock resistant, phenolic-foam...

  11. Parasites causing cerebral falciparum malaria bind multiple endothelial receptors and express EPCR and ICAM-1-binding PfEMP1

    DEFF Research Database (Denmark)

    Tuikue Ndam, Nicaise; Moussiliou, Azizath; Lavstsen, Thomas

    2017-01-01

    Background: Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) mediates the binding and accumulation of infected erythrocytes (IE) to blood vessels and tissues. Specific interactions have been described between PfEMP1 and human endothelial proteins CD36, intercellular adhesion molecule-1...

  12. Structure-Activity Relationships of the Human Immunodeficiency Virus Type 1 Maturation Inhibitor PF-46396.

    Science.gov (United States)

    Murgatroyd, Christopher; Pirrie, Lisa; Tran, Fanny; Smith, Terry K; Westwood, Nicholas J; Adamson, Catherine S

    2016-09-15

    HIV-1 maturation inhibitors are a novel class of antiretroviral compounds that consist of two structurally distinct chemical classes: betulinic acid derivatives and the pyridone-based compound PF-46396. It is currently believed that both classes act by similar modes of action to generate aberrant noninfectious particles via inhibition of CA-SP1 cleavage during Gag proteolytic processing. In this study, we utilized a series of novel analogues with decreasing similarity to PF-46396 to determine the chemical groups within PF-46396 that contribute to antiviral activity, Gag binding, and the relationship between these essential properties. A spectrum of antiviral activity (active, intermediate, and inactive) was observed across the analogue series with respect to CA-SP1 cleavage and HIV-1 (NL4-3) replication kinetics in Jurkat T cells. We demonstrate that selected inactive analogues are incorporated into wild-type (WT) immature particles and that one inactive analogue is capable of interfering with PF-46396 inhibition of CA-SP1 cleavage. Mutations that confer PF-46396 resistance can impose a defective phenotype on HIV-1 that can be rescued in a compound-dependent manner. Some inactive analogues retained the capacity to rescue PF-46396-dependent mutants (SP1-A3V, SP1-A3T, and CA-P157S), implying that they can also interact with mutant Gag. The structure-activity relationships observed in this study demonstrate that (i) the tert-butyl group is essential for antiviral activity but is not an absolute requirement for Gag binding, (ii) the trifluoromethyl group is optimal but not essential for antiviral activity, and (iii) the 2-aminoindan group is important for antiviral activity and Gag binding but is not essential, as its replacement is tolerated. Combinations of antiretroviral drugs successfully treat HIV/AIDS patients; however, drug resistance problems make the development of new mechanistic drug classes an ongoing priority. HIV-1 maturation inhibitors are novel as they

  13. USING THE PARETO DIAGRAM AND FMEA (FAILURE MODE AND EFFECTS ANALYSIS TO IDENTIFY KEY DEFECTS IN A PRODUCT

    Directory of Open Access Journals (Sweden)

    Michał ZASADZIEŃ

    2014-10-01

    Full Text Available The article presents the results of studies conducted in a company manufacturing aluminium forgings for the automotive industry. The aim of the research was to identify the defects which form during the production process as well as the locations and causes of their occurrence. Selected quality management tools were used in the process. Based on the FMEA and the costs generated by the identified defects, a hierarchy of them was created for the company along with a proposal of improvements in case of the most significant ones in order to reduce their number and increase the detection efficiency.

  14. Status of potential PfATP6 molecular markers for artemisinin resistance in Suriname

    Directory of Open Access Journals (Sweden)

    Adhin Malti R

    2012-09-01

    Full Text Available Abstract Background Polymorphisms within the PfATP6 gene have been indicated as potential molecular markers for artemisinin efficacy. Since 2004, the use of artemisinin combination therapy (ACT was introduced as first-line treatment of the uncomplicated malaria cases in Suriname. The aim of this research was to determine changes in Suriname in the status of the polymorphic markers in the PfATP6 gene before and after the adoption of the ACT-regimen, particularly of the S769N mutation, which was reported to be associated with in vitro Artemether resistance in the neighboring country French Guiana. Methods The PfATP6 gene from Plasmodium falciparum parasites in Suriname was investigated in 28 samples using PCR amplification and restriction enzyme analysis, to assess and determine the prevalence of potentially interesting single nucleotide polymorphisms. The polymorphisms [L263E; A623E; S769N], which may be associated with the artemisinin resistant phenotype were characterized in parasites from three endemic regions before and after the adoption of the ACT-regimen. In addition, the status of these molecular markers was compared in paired P. falciparum isolates from patients with recurring malaria after controlled ACT. Results All the investigated samples exhibit the wild-type genotype at all three positions; L263, A623, S769. Conclusion All investigated isolates before and after the adoption of the ACT-regimen and independent of endemic region harbored the wild-type genotype for the three investigated polymorphisms. The study revealed that decreased artemisinin susceptibility could occur independent from PfATP6 mutations, challenging the assumption that artemisinin resistance is associated with these mutations in the PfATP6 gene.

  15. The comparability of the constructs of the 16PF in the South African context

    Directory of Open Access Journals (Sweden)

    F. Abrahams

    1999-06-01

    Full Text Available The primary aims of the study were to determine whether the scores of the 16PF (SA92 are comparable in a crosscultural setting in South Africa, and also to investigate the influence of the gender of the research participants on the scores of the 16PF (SA92. The sample consisted of 983 students who were enrolled at different universities at the time of the field work. The statistical methods involved the application of descriptive statistics as well as methods to determine the comparability of the constructs. The results showed that although race exercised a considerable influence on the findings, this was not necessarily the case as far as gender was concerned. The presence of problems relating to the construct comparability of the test were also identified, as significant differences in means were found between the different race groups. Some of the implications of persisting with the use of the 16PF (SA92 in the South African context are outlined against the background of recently promulgated labour legislation. Opsomming Die primere doelstellings met die onderhawige studie was om die toepaslikheid van die 16PF (SA92 in Suid Afrikaanse konteks met sy diversiteit van kultuurgroepe te evalueer. Die invloed van die geslag van die navorsingsdeelnembers op die resultate is ook nagevors. Die deelnemergroep het bestaan uit 983 studente wat ten tye van die veldwerk aan verskillende universiteite gestudeer het. Benewens normale beskrywende statistiek, is daar ook gebruik gemaak van metodes om konstrukvergelykbaarheid te evalueer. Die bevindings het daarop gedui dat alhoewel ras 'n belangrike invloede op die toetsfaktore uitoefen, dit nie die geval is in soverre dit geslag betref nie. Die teenwoordigheid van probleme het ook ten opsigte van konstrukvergelykbaarheid na vore getree, aangesien betekenis voile verskille tussen gemiddeldes gevind is. Sommige van die implikasies van die volgehoue gebruik van die 16PF (SA92 in Suid Afrika word ook in die lig

  16. An infrared and Raman study of the reorientational motions and phase transition in and structure of CsPF6

    Science.gov (United States)

    Heyns, Anton M.; de Waal, Danita

    1992-12-01

    CsPF6I is face-centered cubic and the PF6- ions are randomly distributed among the six equivalent orientations in the crystal. The ions librate in equilibrium positions for an average time τ, and also jump from one orientation to another in a time which is much shorter than τ. The six different orientations in CsPF6I are of much lower symmetry than the full molecular one. The full vibrational spectra of CsPF6 are reported over the temperature range of 300-4 K and can be analyzed in Phase I in terms of (Oh,C¯4v) where C¯4v denotes the symmetry of the ion and Oh that of the site symmetry S. The polarized Raman spectra of single crystals of CsPF6I are interpreted in terms of all the different orientations of the PF6- ions and excellent agreement is obtained between predicted and experimental results. At 90 K CsPF6 undergoes a phase transition and a crystal structure of P4/nmm-D4h7 is assigned to Phase II. This transition is of the second order and shows behavior which corresponds to those of critical phenomena. An order parameter ξ is defined and a critical constant β is calculated to be equal to 0.3. In CsPF6II two orientations of the PF6- ions exist, both conforming to 4mm-C4v symmetry and the disorder persisting in this phase causes the splitting of the vibrational bands to be fully resolved below 60 K only. An activation energy of ˜3.0 kJ/mol is calculated for the motions of the PF6- ions between 90 and 60 K from the variation of the linewidths and intensities of vibrational bands.

  17. Effect of selective and nonselective beta-blockers on resting energy production rate and total body substrate utilization in chronic heart failure.

    Science.gov (United States)

    Podbregar, Matej; Voga, Gorazd

    2002-12-01

    In chronic heart failure (CHF) beta-blockers reduce myocardial oxygen consumption and improve myocardial efficiency by shifting myocardial substrate utilization from increased free fatty acid oxidation to increased glucose oxidation. The effect of selective and nonselective beta-blockers on total body resting energy production rate (EPR) and substrate utilization is not known. Twenty-six noncachectic patients with moderately severe heart failure (New York Heart Association class II or III, left ventricular ejection fraction < 0.40) were treated with carvedilol (37.5 +/- 13.5 mg/12 h) or bisoprolol (5.4 +/- 3.0 mg/d) for 6 months. Indirect calorimetry was performed before and after 6 months of treatment. Resting EPR was decreased in carvedilol (5.021 +/- 0.803 to 4.552 +/- 0.615 kJ/min, P <.001) and bisoprolol group (5.230 +/- 0.828 to 4.978 +/- 0.640 kJ/min, P <.05; nonsignificant difference between groups). Lipid oxidation rate decreased in carvedilol and remained unchanged in bisoprolol group (2.4 +/- 1.4 to 1.5 +/- 0.9 mg m(2)/kg min versus 2.7 +/- 1.1 to 2.5 +/- 1.1 mg m(2)/kg min, P <.05). Glucose oxidation rate was increased only in carvedilol (2.6 +/- 1.4 to 4.4 +/- 1.6 mg m(2)/kg min, P <.05), but did not change in bisoprolol group. Both selective and nonselective beta-blockers reduce total body resting EPR in noncachectic CHF patients. Carvedilol compared to bisoprolol shifts total body substrate utilization from lipid to glucose oxidation.

  18. Failure Modes

    DEFF Research Database (Denmark)

    Jakobsen, K. P.; Burcharth, H. F.; Ibsen, Lars Bo

    1999-01-01

    The present appendix contains the derivation of ten different limit state equations divided on three different failure modes. Five of the limit state equations can be used independently of the characteristics of the subsoil, whereas the remaining five can be used for either drained or undrained s...

  19. Autotaxin inhibition with PF8380 enhances the radiosensitivity of human and murine glioblastoma cell lines

    Directory of Open Access Journals (Sweden)

    Sandeep R Bhave

    2013-09-01

    Full Text Available Purpose: Glioblastoma multiforme (GBM is an aggressive primary brain tumor that is radio-resistant and recurs despite aggressive surgery, chemo and radiotherapy. Autotaxin (ATX is over expressed in various cancers including GBM and is implicated in tumor progression, invasion, and angiogenesis. Using the ATX specific inhibitor, PF-8380, we studied ATX as a potential target to enhance radiosensitivity in GBM.Methods and Materials: Mouse GL-261 and Human U87MG cells were used as GBM cell models. Clonogenic survival assays and tumor transwell invasion assays were performed using PF-8380 to evaluate role of ATX in survival and invasion. Radiation dependent activation of Akt was analyzed by immunoblotting. Tumor induced angiogenesis was studied using the dorsal skin-fold model in Gl-261. Heterotopic mouse GL-261 tumors were used to evaluate the efficacy of PF-8380 as a radiosensitizer.Results: Pretreatment of GL-261 and U87-MG cells with 1µM PF-8380 followed by 4Gy irradiation resulted in decreased clonogenic survival, decreased migration (33% in GL-261;P = 0.002 and 17.9% in U87; P = 0.012 decreased invasion (35.6% in GL-261; P = 0.0037 and 31.8% in U87; P = 0.002, and attenuated radiation induced Akt phosphorylation. In the tumor window model inhibition of ATX abrogated radiation-induced tumor neovascularization (65%; P=0.011. In a heterotopic mouse GL-261 tumors untreated mice took 11.2 days to reach a tumor volume of 7000 mm3 , however combination of PF-8380 (10mg/kg with irradiation (5 fractions of 2Gy took more than 32 days to reach a tumor volume of 7000 mm3 .Conclusion: Inhibition of ATX by PF8380 led to decreased invasion and enhanced radiosensitization of glioma cells. Radiation induced activation of Akt was abrogated by inhibition of ATX. Furthermore, inhibition of ATX led to diminished tumor vascularity and delayed tumor growth. These results suggest that inhibition of ATX may ameliorate glioblastoma response to radiotherapy.

  20. Accelerated reliability demonstration under competing failure modes

    International Nuclear Information System (INIS)

    Luo, Wei; Zhang, Chun-hua; Chen, Xun; Tan, Yuan-yuan

    2015-01-01

    The conventional reliability demonstration tests are difficult to apply to products with competing failure modes due to the complexity of the lifetime models. This paper develops a testing methodology based on the reliability target allocation for reliability demonstration under competing failure modes at accelerated conditions. The specified reliability at mission time and the risk caused by sampling of the reliability target for products are allocated for each failure mode. The risk caused by degradation measurement fitting of the target for a product involving performance degradation is equally allocated to each degradation failure mode. According to the allocated targets, the accelerated life reliability demonstration test (ALRDT) plans for the failure modes are designed. The accelerated degradation reliability demonstration test plans and the associated ALRDT plans for the degradation failure modes are also designed. Next, the test plan and the decision rules for the products are designed. Additionally, the effects of the discreteness of sample size and accepted number of failures for failure modes on the actual risks caused by sampling for the products are investigated. - Highlights: • Accelerated reliability demonstration under competing failure modes is studied. • The method is based on the reliability target allocation involving the risks. • The test plan for the products is based on the plans for all the failure modes. • Both failure mode and degradation failure modes are considered. • The error of actual risks caused by sampling for the products is small enough

  1. Suppressive Effects of the Site 1 Protease (S1P Inhibitor, PF-429242, on Dengue Virus Propagation

    Directory of Open Access Journals (Sweden)

    Leo Uchida

    2016-02-01

    Full Text Available Dengue virus (DENV infection causes one of the most widespread mosquito-borne diseases in the world. Despite the great need, effective vaccines and practical antiviral therapies are still under development. Intracellular lipid levels are regulated by sterol regulatory elements-binding proteins (SREBPs, which are activated by serine protease, site 1 protease (S1P. Small compound PF-429242 is known as a S1P inhibitor and the antivirus effects have been reported in some viruses. In this study, we examined the anti-DENV effects of PF-429242 using all four serotypes of DENV by several primate-derived cell lines. Moreover, emergence of drug-resistant DENV mutants was assessed by sequential passages with the drug. DENV dependency on intracellular lipids during their infection was also evaluated by adding extracellular lipids. The addition of PF-429242 showed suppression of viral propagation in all DENV serotypes. We showed that drug-resistant DENV mutants are unlikely to emerge after five times sequential passages through treatment with PF-429242. Although the levels of intracellular cholesterol and lipid droplets were reduced by PF-429242, viral propagations were not recovered by addition of exogenous cholesterol or fatty acids, indicating that the reduction of LD and cholesterol caused by PF-429242 treatment is not related to its mechanism of action against DENV propagation. Our results suggest that PF-429242 is a promising candidate for an anti-DENV agent.

  2. A homeodomain transcription factor gene, PfMSX, activates expression of Pif gene in the pearl oyster Pinctada fucata.

    Directory of Open Access Journals (Sweden)

    Mi Zhao

    Full Text Available We reported pearl oyster Pinctada fucata cDNA and genomic characterization of a new homeobox-containing protein, PfMSX. The PfMSX gene encodes a transcription factor that was localized to the nucleus. Analyses of PfMSX mRNA in tissues and developmental stages showed high expressions in mantle or D-shaped larvae. In electrophoretic mobility shift assays (EMSAs PfMSX binded to MSX consensus binding sites in the 5' flanking region of the Pif promoter. In co-transfection experiment PfMSX transactivated reporter constructs containing Pif promoter sequences, and mutation of the MSX-binding sites attenuated transactivation. A knockdown experiment using PfMSX dsRNA showed decreased Pif mRNA and unregular crystallization of the nacreous layer using scanning electron microscopy. Our results suggested that PfMSX was a conserved homeodomain transcription factor gene, which can activate Pif gene expression through MSX binding site, and was then involved in the mineralization process in pearl oyster Pinctada fucata. Our data provided important clues about mechanisms regulating biomineralization in pearl oyster.

  3. A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata

    Science.gov (United States)

    Zhao, Mi; He, Maoxian; Huang, Xiande; Wang, Qi

    2014-01-01

    We reported pearl oyster Pinctada fucata cDNA and genomic characterization of a new homeobox-containing protein, PfMSX. The PfMSX gene encodes a transcription factor that was localized to the nucleus. Analyses of PfMSX mRNA in tissues and developmental stages showed high expressions in mantle or D-shaped larvae. In electrophoretic mobility shift assays (EMSAs) PfMSX binded to MSX consensus binding sites in the 5′ flanking region of the Pif promoter. In co-transfection experiment PfMSX transactivated reporter constructs containing Pif promoter sequences, and mutation of the MSX-binding sites attenuated transactivation. A knockdown experiment using PfMSX dsRNA showed decreased Pif mRNA and unregular crystallization of the nacreous layer using scanning electron microscopy. Our results suggested that PfMSX was a conserved homeodomain transcription factor gene, which can activate Pif gene expression through MSX binding site, and was then involved in the mineralization process in pearl oyster Pinctada fucata. Our data provided important clues about mechanisms regulating biomineralization in pearl oyster. PMID:25099698

  4. A homeodomain transcription factor gene, PfMSX, activates expression of Pif gene in the pearl oyster Pinctada fucata.

    Science.gov (United States)

    Zhao, Mi; He, Maoxian; Huang, Xiande; Wang, Qi

    2014-01-01

    We reported pearl oyster Pinctada fucata cDNA and genomic characterization of a new homeobox-containing protein, PfMSX. The PfMSX gene encodes a transcription factor that was localized to the nucleus. Analyses of PfMSX mRNA in tissues and developmental stages showed high expressions in mantle or D-shaped larvae. In electrophoretic mobility shift assays (EMSAs) PfMSX binded to MSX consensus binding sites in the 5' flanking region of the Pif promoter. In co-transfection experiment PfMSX transactivated reporter constructs containing Pif promoter sequences, and mutation of the MSX-binding sites attenuated transactivation. A knockdown experiment using PfMSX dsRNA showed decreased Pif mRNA and unregular crystallization of the nacreous layer using scanning electron microscopy. Our results suggested that PfMSX was a conserved homeodomain transcription factor gene, which can activate Pif gene expression through MSX binding site, and was then involved in the mineralization process in pearl oyster Pinctada fucata. Our data provided important clues about mechanisms regulating biomineralization in pearl oyster.

  5. A Novel Acidic Matrix Protein, PfN44, Stabilizes Magnesium Calcite to Inhibit the Crystallization of Aragonite*

    Science.gov (United States)

    Pan, Cong; Fang, Dong; Xu, Guangrui; Liang, Jian; Zhang, Guiyou; Wang, Hongzhong; Xie, Liping; Zhang, Rongqing

    2014-01-01

    Magnesium is widely used to control calcium carbonate deposition in the shell of pearl oysters. Matrix proteins in the shell are responsible for nucleation and growth of calcium carbonate crystals. However, there is no direct evidence supporting a connection between matrix proteins and magnesium. Here, we identified a novel acidic matrix protein named PfN44 that affected aragonite formation in the shell of the pearl oyster Pinctada fucata. Using immunogold labeling assays, we found PfN44 in both the nacreous and prismatic layers. In shell repair, PfN44 was repressed, whereas other matrix proteins were up-regulated. Disturbing the function of PfN44 by RNAi led to the deposition of porous nacreous tablets with overgrowth of crystals in the nacreous layer. By in vitro circular dichroism spectra and fluorescence quenching, we found that PfN44 bound to both calcium and magnesium with a stronger affinity for magnesium. During in vitro calcium carbonate crystallization and calcification of amorphous calcium carbonate, PfN44 regulated the magnesium content of crystalline carbonate polymorphs and stabilized magnesium calcite to inhibit aragonite deposition. Taken together, our results suggested that by stabilizing magnesium calcite to inhibit aragonite deposition, PfN44 participated in P. fucata shell formation. These observations extend our understanding of the connections between matrix proteins and magnesium. PMID:24302723

  6. Suppressive Effects of the Site 1 Protease (S1P) Inhibitor, PF-429242, on Dengue Virus Propagation.

    Science.gov (United States)

    Uchida, Leo; Urata, Shuzo; Ulanday, Gianne Eduard L; Takamatsu, Yuki; Yasuda, Jiro; Morita, Kouichi; Hayasaka, Daisuke

    2016-02-10

    Dengue virus (DENV) infection causes one of the most widespread mosquito-borne diseases in the world. Despite the great need, effective vaccines and practical antiviral therapies are still under development. Intracellular lipid levels are regulated by sterol regulatory elements-binding proteins (SREBPs), which are activated by serine protease, site 1 protease (S1P). Small compound PF-429242 is known as a S1P inhibitor and the antivirus effects have been reported in some viruses. In this study, we examined the anti-DENV effects of PF-429242 using all four serotypes of DENV by several primate-derived cell lines. Moreover, emergence of drug-resistant DENV mutants was assessed by sequential passages with the drug. DENV dependency on intracellular lipids during their infection was also evaluated by adding extracellular lipids. The addition of PF-429242 showed suppression of viral propagation in all DENV serotypes. We showed that drug-resistant DENV mutants are unlikely to emerge after five times sequential passages through treatment with PF-429242. Although the levels of intracellular cholesterol and lipid droplets were reduced by PF-429242, viral propagations were not recovered by addition of exogenous cholesterol or fatty acids, indicating that the reduction of LD and cholesterol caused by PF-429242 treatment is not related to its mechanism of action against DENV propagation. Our results suggest that PF-429242 is a promising candidate for an anti-DENV agent.

  7. Chemical vapor deposition of NiSi using Ni(PF3)4 and Si3H8

    International Nuclear Information System (INIS)

    Ishikawa, M.; Muramoto, I.; Machida, H.; Imai, S.; Ogura, A.; Ohshita, Y.

    2007-01-01

    NiSi x films were deposited using chemical vapor deposition (CVD) with a Ni(PF 3 ) 4 and Si 3 H 8 /H 2 gas system. The step coverage quality of deposited NiSi x was investigated using a horizontal type of hot-wall low pressure CVD reactor, which maintained a constant temperature throughout the deposition area. The step coverage quality improved as a function of the position of the gas flow direction, where PF 3 gas from decomposition of Ni(PF 3 ) 4 increased. By injecting PF 3 gas into the Ni(PF 3 ) 4 and Si 3 H 8 /H 2 gas system, the step coverage quality markedly improved. This improvement in step coverage quality naturally occurred when PF 3 gas was present, indicating a strong relationship. The Si/Ni deposit ratio at 250 deg. C is larger than at 180 deg. C. It caused a decreasing relative deposition rate of Ni to Si. PF 3 molecules appear to be adsorbed on the surface of the deposited film and interfere with faster deposition of active Ni deposition species

  8. Types of Heart Failure

    Science.gov (United States)

    ... Introduction Types of Heart Failure Classes of Heart Failure Heart Failure in Children Advanced Heart Failure • Causes and ... and procedures related to heart disease and stroke. Heart Failure Questions to Ask Your Doctor Use these questions ...

  9. Classes of Heart Failure

    Science.gov (United States)

    ... Introduction Types of Heart Failure Classes of Heart Failure Heart Failure in Children Advanced Heart Failure • Causes and ... and Advanced HF • Tools and Resources • Personal Stories Heart Failure Questions to Ask Your Doctor Use these questions ...

  10. The Crystal Structure of PF-8, the DNA Polymerase Accessory Subunit from Kaposi's Sarcoma-Associated Herpesvirus

    Energy Technology Data Exchange (ETDEWEB)

    Baltz, Jennifer L.; Filman, David J.; Ciustea, Mihai; Silverman, Janice Elaine Y.; Lautenschlager, Catherine L.; Coen, Donald M.; Ricciardi, Robert P.; Hogle, James M.; (UPENN)

    2009-12-01

    Kaposi's sarcoma-associated herpesvirus is an emerging pathogen whose mechanism of replication is poorly understood. PF-8, the presumed processivity factor of Kaposi's sarcoma-associated herpesvirus DNA polymerase, acts in combination with the catalytic subunit, Pol-8, to synthesize viral DNA. We have solved the crystal structure of residues 1 to 304 of PF-8 at a resolution of 2.8 {angstrom}. This structure reveals that each monomer of PF-8 shares a fold common to processivity factors. Like human cytomegalovirus UL44, PF-8 forms a head-to-head dimer in the form of a C clamp, with its concave face containing a number of basic residues that are predicted to be important for DNA binding. However, there are several differences with related proteins, especially in loops that extend from each monomer into the center of the C clamp and in the loops that connect the two subdomains of each protein, which may be important for determining PF-8's mode of binding to DNA and to Pol-8. Using the crystal structures of PF-8, the herpes simplex virus catalytic subunit, and RB69 bacteriophage DNA polymerase in complex with DNA and initial experiments testing the effects of inhibition of PF-8-stimulated DNA synthesis by peptides derived from Pol-8, we suggest a model for how PF-8 might form a ternary complex with Pol-8 and DNA. The structure and the model suggest interesting similarities and differences in how PF-8 functions relative to structurally similar proteins.

  11. Evasion of immunity to Plasmodium falciparum malaria by IgM masking of protective IgG epitopes in infected erythrocyte surface-exposed PfEMP1

    DEFF Research Database (Denmark)

    Barfod, Lea; Dalgaard, Michael B; Pleman, Suzan T

    2011-01-01

    1 (PfEMP1) family is central to both. Here, we present evidence of a P. falciparum evasion mechanism not previously documented: the masking of PfEMP1-specific IgG epitopes by nonspecific IgM. Nonspecific IgM binding to erythrocytes infected by parasites expressing the PfEMP1 protein VAR2CSA...

  12. Characterization of polyacrylamide-stabilized Pf1 phage liquid crystals for protein NMR spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Trempe, Jean-Francois; Morin, Frederick G.; Xia Zhicheng; Marchessault, Robert H.; Gehring, Kalle [McGill University, Department of Biochemistry and Department of Chemistry (Canada)], E-mail: kalle@bri.nrc.ca

    2002-01-15

    A new polymer-stabilized nematic liquid crystal has been characterized for the measurement of biomolecular residual dipolar couplings. Filamentous Pf1 phage were embedded in a polyacrylamide matrix that fixes the orientation of the particles. The alignment was characterized by the quadrupolar splitting of the {sup 2}H NMR water signal and by the measurement of {sup 1}H-{sup 15}N residual dipolar couplings (RDC) in the archeal translation elongation factor 1{beta}. Protein dissolved in the polymer-stabilized medium orients quantitatively as in media without polyacrylamide. We show that the quadrupolar splitting and RDCs are zero in media in which the Pf1 phage particles are aligned at the magic angle. This allows measurement of J and dipolar couplings in a single sample.

  13. Characterization of polyacrylamide-stabilized Pf1 phage liquid crystals for protein NMR spectroscopy

    International Nuclear Information System (INIS)

    Trempe, Jean-Francois; Morin, Frederick G.; Xia Zhicheng; Marchessault, Robert H.; Gehring, Kalle

    2002-01-01

    A new polymer-stabilized nematic liquid crystal has been characterized for the measurement of biomolecular residual dipolar couplings. Filamentous Pf1 phage were embedded in a polyacrylamide matrix that fixes the orientation of the particles. The alignment was characterized by the quadrupolar splitting of the 2 H NMR water signal and by the measurement of 1 H- 15 N residual dipolar couplings (RDC) in the archeal translation elongation factor 1β. Protein dissolved in the polymer-stabilized medium orients quantitatively as in media without polyacrylamide. We show that the quadrupolar splitting and RDCs are zero in media in which the Pf1 phage particles are aligned at the magic angle. This allows measurement of J and dipolar couplings in a single sample

  14. Review of solution approach, methods, and recent results of the TRAC-PF1 system code

    International Nuclear Information System (INIS)

    Mahaffy, J.H.; Liles, D.R.; Knight, T.D.

    1983-01-01

    The current version of the Transient Reactor Analysis Code (TRAC-PF1) was created to improve on the capabilities of its predecessor (TRAC-PD2) for analyzing slow reactor transients such as small-break loss-of-coolant accidents. TRAC-PF1 continues to use a semi-implicit finite-difference method for modeling three-dimensional flows in the reactor vessel. However, it contains a new stability-enhancing two-step (SETS) finite-difference tecnique for one-dimensional flow calculations. This method is not restricted by a material Courant stability condition, allowing much larger time-step sizes during slow transients than would a semi-implicit method. These have been successfully applied to the analysis of a variety of experiments and hypothetical plant transients covering a full range of two-phase flow regimes

  15. [BMIM][PF(6)] promotes the synthesis of halohydrin esters from diols using potassium halides.

    Science.gov (United States)

    Oromí-Farrús, Mireia; Eras, Jordi; Villorbina, Gemma; Torres, Mercè; Llopis-Mestre, Veronica; Welton, Tom; Canela, Ramon

    2008-10-01

    Haloesterification of diverse diols with various carboxylic acids was achieved using potassium halides (KX) as the only halide source in ionic liquids. The best yield was obtained in [BMIM][PF(6)] when 1,2-octanediol, palmitic acid and KBr were used. This yield was 85% and the regioisomer with the bromine in primary position was present in a 75:25 ratio. The regioisomeric ratio could be improved using either KCl or some phenylcarboxylic acids. [BMIM][PF(6)] acts as both reaction media and catalyst of the reaction. To the best of our knowledge, this type of combined reaction using an ionic liquid is unprecedented. The other solvents tested did not lead either to the same yield or to the same regioisomeric ratio.

  16. Anisotropic p-f mixing mechanism explaining anomalous magnetic properties in Ce monopnictides

    International Nuclear Information System (INIS)

    Takahashi, H.; Kasuya, T.

    1985-01-01

    The indirect f-f-interaction derived from fourth-order perturbation theory with respect to the p-f mixing is calculated using the valence bands obtained by the APW band calculations. The type of the f-f-interactions is described as the coupling through the symmetry exchange under the cubic crystal field, which cannot be written as a simple bilinear type of 4f spin operator. It is necessary to consider the short-range-ordering effect as well as the non-linear effect of the p-f mixing to explain the fact that a type-I antiferromagnetic ordering is established by the second-order transition in CeBi. (author)

  17. Measurements of energy spectra of fast electrons from PF-1000 in the upstream and downstream directions

    Energy Technology Data Exchange (ETDEWEB)

    Kwiatkowski, R.; Czaus, K.; Skladnik-Sadowska, E.; Malinowski, K.; Zebrowski, J. [The Andrzej Soltan Institute for Nuclear Studies (IPJ), 05-400 Otwock-Swierk (Poland); Sadowski, M.J. [The Andrzej Soltan Institute for Nuclear Studies (IPJ), 05-400 Otwock-Swierk (Poland); Karpinski, L.; Paduch, M.; Scholz, M. [Institute of Plasma Physics and Laser Microfusion (IPPLM), 01-497 Warsaw (Poland); Kubes, P. [Czech Technical University (CVUT), 166-27 Prague, (Czech Republic)

    2011-07-01

    The paper describes measurements of energy spectra of electrons emitted in the upstream direction along the symmetry-axis of the PF-1000 facility, operated with the deuterium filling at 21 kV, 290 kJ. The measurements were performed with a magnetic analyzer. The same analyzer was used to measure also electron beams emitted in along the symmetry-axis in the downstream direction. The recorded spectra showed that the electron-beams emitted in the upstream direction have energies in the range from about 40 keV to about 800 keV, while those in the downstream direction have energies in the range from about 60 keV to about 200 keV. These spectra confirm that in the PF (Plasma Focus) plasma column there appear strong local fields accelerating charged particles in different directions. This document is composed of a paper and a poster. (authors)

  18. Synthesis of Ca(PF$_6$)$_2$, formed $\\textit{via}$ nitrosonium oxidation of calcium

    OpenAIRE

    Keyzer, Evan; Matthews, PD; Liu, Zigeng; Bond, Andrew David; Grey, Clare Philomena; Wright, Dominic Simon

    2017-01-01

    The development of rechargeable Ca-ion batteries as an alternative to Li systems has been limited by the availability of suitable electrolyte salts. We present the synthesis of complexes of Ca(PF$_6$)$_2$ (a key potential Ca battery electrolyte salt) $\\textit{via}$ the treatment of Ca metal with NOPF$_6$, and explore their conversion to species containing PO$_2$F$_2$$^{-}$ under the reaction conditions. NSERC of Canada PGSD; Gonville and Caius College; Cambridge Commonwealth, European, and...

  19. Synthesis of Ca(PF6)2, formed via nitrosonium oxidation of calcium.

    Science.gov (United States)

    Keyzer, Evan N; Matthews, Peter D; Liu, Zigeng; Bond, Andrew D; Grey, Clare P; Wright, Dominic S

    2017-04-20

    The development of rechargeable Ca-ion batteries as an alternative to Li systems has been limited by the availability of suitable electrolyte salts. We present the synthesis of complexes of Ca(PF 6 ) 2 (a key potential Ca battery electrolyte salt) via the treatment of Ca metal with NOPF 6 , and explore their conversion to species containing PO 2 F 2 - under the reaction conditions.

  20. Low temperature specific heat of the spin-density-wave compound (TMTSF)2PF6

    DEFF Research Database (Denmark)

    Odin, J.; Lasjaunias, J.C.; Biljakovic, K.

    1994-01-01

    We report on specific heat measurements of the SDW compound (TMTSF)2PF6 between 2 and 25 K, performed by two different techniques. We discuss the two successive transitions which occur in this T-range : the SDW ordering transition at T = 12.1 K, and a glass transition around-3-3.5 K. The latter i...... is very dependent on the kinetics of measurements, and has all characteristic features of freezing of supercooled liquids....

  1. Artificial intelligence and distance learning philosophy in support of PfP mandate

    OpenAIRE

    Antoliš, Krunoslav

    2003-01-01

    Computers have long been utilised in the legal environment. The main use of computers however, has merely been to automate office tasks. More exciting is the prospect of using artificial intelligence (AI) technology to create computers that can emulate the substantive legal jobs performed by lawyers, to create computers that can autonomously reason with the law to determine legal solutions, for example: structuring and support of Partnership for Peace (PfP) mandate. Such attempts have not bee...

  2. Phebus PF, international major programme in nuclear safety: the last test

    International Nuclear Information System (INIS)

    2004-11-01

    For the last test Phebus P.F. (the fifth one), it is expected a fast degradation of the boron carbon pencils, leading to a fast release of boron and carbon gas species. Among the carbon gas produced, it is expected to a presence of methane susceptible to interact with iodine to form organic species. The conditions of tests should lead to observe a contribution of containment sump to volatile iodine generation. (N.C.)

  3. Design study on a high brilliance lattice of the PF storage ring

    International Nuclear Information System (INIS)

    Katoh, M.; Araki, A.; Kobayashi, Y.; Hori, Y.

    1994-01-01

    A high brilliance lattice of the PF storage ring is proposed. A small beam emittance of 27 nm-rad (about one fifth of the present value) can be achieved by doubling the number of the quadrupoles in the FODO cells. This emittance reduction will result in ten times brighter synchrotron light from the existing insertion devices. The problems incidental to the low emittance lattice, the small dynamic aperture and the short Touschek lifetime, will be discussed. (author)

  4. Failure Analysis

    International Nuclear Information System (INIS)

    Iorio, A.F.; Crespi, J.C.

    1987-01-01

    After ten years of operation at the Atucha I Nuclear Power Station a gear belonging to a pressurized heavy water reactor refuelling machine, failed. The gear box was used to operate the inlet-outlet heavy-water valve of the machine. Visual examination of the gear device showed an absence of lubricant and that several gear teeth were broken at the root. Motion was transmitted with a speed-reducing device with controlled adjustable times in order to produce a proper fitness of the valve closure. The aim of this paper is to discuss the results of the gear failure analysis in order to recommend the proper solution to prevent further failures. (Author)

  5. Implementation of JAERI's reflood model into TRAC-PF1/MOD1 code

    International Nuclear Information System (INIS)

    Akimoto, Hajime; Ohnuki, Akira; Murao, Yoshio

    1993-02-01

    Selected physical models of REFLA code, that is a reflood analysis code developed at JAERI, were implemented into the TRAC-PF1/MOD1 code in order to improve the predictive capability of the TRAC-PF1/MOD1 code for the core thermal hydraulic behaviors during the reflood phase in a PWR LOCA. Through comparisons of physical models between both codes, (1) Murao-Iguchi void fraction correlation, (2) the drag coefficient correlation acting to drops, (3) the correlation for wall heat transfer coefficient in the film boiling regime, (4) the quench velocity correlation and (5) heat transfer correlations for the dispersed flow regime were selected from the REFLA code to be implemented into the TRAC-PF1/MOD1 code. A method for the transformation of the void fraction correlation to the equivalent interfacial friction model was developed and the effect of the transformation method on the stability of the solution was discussed. Through assessment calculation using data from CCTF (Cylindrical Core Test Facility) flat power test, it was confirmed that the predictive capability of the TRAC code for the core thermal hydraulic behaviors during the reflood can be improved by the implementation of selected physical models of the REFLA code. Several user guidelines for the modified TRAC code were proposed based on the sensitivity studies on fluid cell number in the hydraulic calculation and on node number and effect of axial heat conduction in the heat conduction calculation of fuel rod. (author)

  6. Assessment of TRAC-PF1/MOD1 code for large break LOCA in PWR

    International Nuclear Information System (INIS)

    Akimoto, Hajime; Ohnuki, Akira; Murao, Yoshio; Abe, Yutaka.

    1993-03-01

    As the first step of the REFLA/TRAC code development, the TRAC/PF1/MOD1 code has been assessed for various experiments that simulate postulated large-break loss-of-coolant accident (LBLOCA) in PWR to understand the predictive capability and to identify the problem areas of the code. The assessment calculations were performed for separate effect tests for critical flow, counter current flow, condensation at cold leg and reflood as well as integral tests to understand predictability for individual phenomena. This report summarizes results from the assessment calculations of the TRAC-PF1/MOD1 code for LBLOCA in PWR. The assessment calculations made clear the predictive capability and problem areas of the TRAC-PF1/MOD1 code for LBLOCA in PWR. The areas, listed below, should be improved for more realistic and effective simulation of LBLOCA in PWR: (1) core heat transfer model during blowdown, (2) ECC bypass model at downcomer during refill, (3) condensation model during accumulator injection, and (4) core thermal hydraulic model during reflood. (author) 57 refs

  7. Mutations in the Plasmodium falciparum Cyclic Amine Resistance Locus (PfCARL Confer Multidrug Resistance

    Directory of Open Access Journals (Sweden)

    Gregory LaMonte

    2016-07-01

    Full Text Available Mutations in the Plasmodium falciparum cyclic amine resistance locus (PfCARL are associated with parasite resistance to the imidazolopiperazines, a potent class of novel antimalarial compounds that display both prophylactic and transmission-blocking activity, in addition to activity against blood-stage parasites. Here, we show that pfcarl encodes a protein, with a predicted molecular weight of 153 kDa, that localizes to the cis-Golgi apparatus of the parasite in both asexual and sexual blood stages. Utilizing clustered regularly interspaced short palindromic repeat (CRISPR-mediated gene introduction of 5 variants (L830V, S1076N/I, V1103L, and I1139K, we demonstrate that mutations in pfcarl are sufficient to generate resistance against the imidazolopiperazines in both asexual and sexual blood-stage parasites. We further determined that the mutant PfCARL protein confers resistance to several structurally unrelated compounds. These data suggest that PfCARL modulates the levels of small-molecule inhibitors that affect Golgi-related processes, such as protein sorting or membrane trafficking, and is therefore an important mechanism of resistance in malaria parasites.

  8. Quantum Hall effect and anomalous transport in (TMTSF)2PF6

    International Nuclear Information System (INIS)

    Eom, J.; Cho, H.; Kang, W.; Chicago Univ., IL

    1999-01-01

    Under low temperatures and high magnetic fields, quasi-one dimensional organic conductor (TMTSFP) 2 PF 6 exhibits a series of transitions to field-induced spin density wave (FISDW). Slightly above the onset of superconductivity in (TMTSF) 2 PF 6 , we observe a series of intervening phases that interrupt the sequence of FISDW that gives rise to the quantum Hall effect. These phases can be identified either as negative quantum numbered FISDW states or a puzzling arboresecent phase. Detailed study of the QHE in (TMTSF) 2 PF 6 reveals that the transport in the FISDW phases is dominated by anomalous longitudinal resistivities ρ xx and ρ yy that remain finite at low temperatures. While the quantization of σ xy is not adversely affected at high magnetic fields, the transport in the intermediate magnetic field remains complicated. In addition, the conductivity along applied magnetic field, σ zz , cannot be easily understood in terms of three-dimensional QHE and is suggestive of the importance of inter-layer coupling. (orig.)

  9. QSAR study on the antimalarial activity of Plasmodium falciparum dihydroorotate dehydrogenase (PfDHODH) inhibitors.

    Science.gov (United States)

    Hou, X; Chen, X; Zhang, M; Yan, A

    2016-01-01

    Plasmodium falciparum, the most fatal parasite that causes malaria, is responsible for over one million deaths per year. P. falciparum dihydroorotate dehydrogenase (PfDHODH) has been validated as a promising drug development target for antimalarial therapy since it catalyzes the rate-limiting step for DNA and RNA biosynthesis. In this study, we investigated the quantitative structure-activity relationships (QSAR) of the antimalarial activity of PfDHODH inhibitors by generating four computational models using a multilinear regression (MLR) and a support vector machine (SVM) based on a dataset of 255 PfDHODH inhibitors. All the models display good prediction quality with a leave-one-out q(2) >0.66, a correlation coefficient (r) >0.85 on both training sets and test sets, and a mean square error (MSE) antimalarial activity. The models are capable of predicting inhibitors' antimalarial activity and the molecular descriptors for building the models could be helpful in the development of new antimalarial drugs.

  10. Chlorpheniramine Analogues Reverse Chloroquine Resistance in Plasmodium falciparum by Inhibiting PfCRT.

    Science.gov (United States)

    Deane, Karen J; Summers, Robert L; Lehane, Adele M; Martin, Rowena E; Barrow, Russell A

    2014-05-08

    The emergence and spread of malaria parasites that are resistant to chloroquine (CQ) has been a disaster for world health. The antihistamine chlorpheniramine (CP) partially resensitizes CQ-resistant (CQR) parasites to CQ but possesses little intrinsic antiplasmodial activity. Mutations in the parasite's CQ resistance transporter (PfCRT) confer resistance to CQ by enabling the protein to transport the drug away from its site of action, and it is thought that resistance-reversers such as CP exert their effect by blocking this CQ transport activity. Here, a series of new structural analogues and homologues of CP have been synthesized. We show that these compounds (along with other in vitro CQ resistance-reversers) inhibit the transport of CQ via a resistance-conferring form of PfCRT expressed in Xenopus laevis oocytes. Furthermore, the level of PfCRT-inhibition was found to correlate well with both the restoration of CQ accumulation and the level of CQ resensitization in CQR parasites.

  11. Genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii

    International Nuclear Information System (INIS)

    Dutcher, S.K.; Gibbons, W.; Inwood, W.B.

    1988-01-01

    A mutation at the PF10 locus of the unicellular green alga Chlamydomonas reinhardtii leads to abnormal cell motility. The asymmetric form of the ciliary beat stroke characteristic of wild-type flagella is modified by this mutation to a nearly symmetric beat. We report here that this abnormal motility is a conditional phenotype that depends on light intensity. In the absence of light or under low light intensities, the motility is more severely impaired than at higher light intensities. By UV mutagenesis we obtained 11 intragenic and 70 extragenic strains that show reversion of the pf10 motility phenotype observed in low light. The intragenic events reverted the motility phenotype of the pf10 mutation completely. The extragenic events define at least seven suppressor loci; these map to linkage groups IV, VII, IX, XI, XII and XVII. Suppressor mutations at two of the seven loci (LIS1 and LIS2) require light for their suppressor activity. Forty-eight of the 70 extragenic suppressors were examined in heterozygous diploid cells; 47 of these mutants were recessive to the wild-type allele and one mutant (bop5-1) was dominant to the wild-type allele. Complementation analysis of the 47 recessive mutants showed unusual patterns. Most mutants within a recombinationally defined group failed to complement one another, although there were pairs that showed intra-allelic complementation. Additionally, some of the mutants at each recombinationally defined locus failed to complement mutants at other loci. They define dominant enhancers of one another

  12. Numerical study for determining PF coil system parameters in MHD equilibrium of KT-2 tokamak plasma

    International Nuclear Information System (INIS)

    Ryu, J.; Hong, S.H.; Lee, K.W.; Hong, B.G.; In, S.R.; Kim, S.K.

    1995-01-01

    The KT-2 is a large-aspect-ratio medium-sized divertor tokamak in the conceptual design phase and planned to be operational in 1998 at the Korea Atomic Energy Research Institute (KAERI). Plasma equilibrium in tokamak can be acquired by controlling the current of poloidal field (PF) coils in appropriate geometries and positions. In this study, the authors have performed numerical calculations to achieve the various equilibrium conditions fitting given plasma shapes and satisfying PF current limitations. Usually an ideal magnetohydrodynamic (MHD) equation is used to obtain the equilibrium solution of tokamak plasma, and it is practical to take advantage of a numerical method in solving the MHD equation because it has nonlinear source terms. Two equilibrium codes have been applied to find a double-null configuration of free-boundary tokamak plasma in KT-2: one is of the authors' own developing and the other is a free-boundary tokamak equilibrium code (FBT) that has been used mainly for the verification of developed code's results. PF coil system parameters including their positions and currents are determined for the optimization of input power required when the specifications of KT-2 tokamak are met. Then, several sets of equilibrium conditions during the tokamak operation are found to observe the changes of poloidal field currents with the passing of operation time step, and the basic stability problems related with the magnetic field structure is also considered

  13. Failure analysis of buried tanks

    International Nuclear Information System (INIS)

    Watkins, R.K.

    1994-01-01

    Failure of a buried tank can be hazardous. Failure may be a leak through which product is lost from the tank; but also through which contamination can occur. Failures are epidemic -- because buried tanks are out of sight, but also because designers of buried tanks have adopted analyses developed for pressure tanks. So why do pressure tanks fail when they are buried? Most failures of buried tanks are really soil failures. Soil compresses, or slips, or liquefies. Soil is not only a load, it is a support without which the tank deforms. A high water table adds to the load on the tank. It also reduces the strength of the soil. Based on tests, structural analyses are proposed for empty tanks buried in soils of various quality, with the water table at various levels, and with internal vacuum. Failure may be collapse tank. Such collapse is a sudden, audible inversion of the cylinder when the sidefill soil slips. Failure may be flotation. Failure may be a leak. Most leaks are fractures in the welds in overlap seams at flat spots. Flat spots are caused by a hard bedding or a heavy surface wheel load. Because the tank wall is double thick at the overlap, shearing stress in the weld is increased. Other weld failures occur when an end plate shears down past a cylinder; or when the tank is supported only at its ends like a beam. These, and other, failures can be analyzed with justifiable accuracy using basic principles of mechanics of materials. 10 figs

  14. About PF

    Science.gov (United States)

    ... make scar tissue. Fibrosis : Scar tissue. Forced expiratory volume (FEV1): The amount of air you can blow out oin one second after filling up your lungs with as much air as possible. Measured by a test called spirometry. Forced vital capacity (FVC): The amount of air you can blow ...

  15. Effects of cryogenic temperature on the mechanical and failure characteristics of melamine-urea-formaldehyde adhesive plywood

    Science.gov (United States)

    Kim, Jeong-Hyeon; Choi, Sung-Woong; Park, Doo-Hwan; Park, Seong-Bo; Kim, Seul-Kee; Park, Kwang-Jun; Lee, Jae-Myung

    2018-04-01

    The present study investigates the applicability of melamine-urea-formaldehyde (MUF) resin plywood in cryogenic applications, including liquefied natural gas (LNG) carrier insulation systems. Phenolic-formaldehyde (PF) resin plywood has been extensively used as a structural material in industrial applications. However, many shortcomings of PF resin plywood have been reported, and replacement of PF resin plywood with a new material is necessary to resolve these problems. MUF resin plywood has the advantages of short fabrication time, low veneer cost, and economic feasibility compared to PF resin plywood. However, the mechanical and failure characteristics of MUF resin plywood have not yet been investigated at low temperature ranges. For this reason, adapting MUF resin plywood for cryogenic applications has been difficult, despite the many strong points of the material in engineering aspects. In this study, the effects of cryogenic temperature and thermal treatment on the mechanical characteristics of MUF resin plywood are investigated. The performance of MUF resin plywood is compared with that of PF resin plywood to verify the applicability of the material for use as a structural material in LNG insulation systems. The results demonstrate that MUF resin plywood has mechanical properties comparable with those of PF resin plywood, even at cryogenic conditions.

  16. Epithelial tissue hyperplasia induced by the RAF inhibitor PF-04880594 is attenuated by a clinically well-tolerated dose of the MEK inhibitor PD-0325901.

    Science.gov (United States)

    Torti, Vince R; Wojciechowicz, Donald; Hu, Wenyue; John-Baptiste, Annette; Evering, Winston; Troche, Gabriel; Marroquin, Lisa D; Smeal, Tod; Yamazaki, Shinji; Palmer, Cynthia L; Burns-Naas, Leigh Ann; Bagrodia, Shubha

    2012-10-01

    Clinical trials of selective RAF inhibitors in patients with melanoma tumors harboring activated BRAFV600E have produced very promising results, and a RAF inhibitor has been approved for treatment of advanced melanoma. However, about a third of patients developed resectable skin tumors during the course of trials. This is likely related to observations that RAF inhibitors activate extracellular signal-regulated kinase (ERK) signaling, stimulate proliferation, and induce epithelial hyperplasia in preclinical models. Because these findings raise safety concerns about RAF inhibitor development, we further investigated the underlying mechanisms. We showed that the RAF inhibitor PF-04880594 induces ERK phosphorylation and RAF dimerization in those epithelial tissues that undergo hyperplasia. Hyperplasia and ERK hyperphosphorylation are prevented by treatment with the mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitor PD-0325901 at exposures that extrapolate to clinically well-tolerated doses. To facilitate mechanistic and toxicologic studies, we developed a three-dimensional cell culture model of epithelial layering that recapitulated the RAF inhibitor-induced hyperplasia and reversal by MEK inhibitor in vitro. We also showed that PF-04880594 stimulates production of the inflammatory cytokine interleukin 8 in HL-60 cells, suggesting a possible mechanism for the skin flushing observed in dogs. The complete inhibition of hyperplasia by MEK inhibitor in epithelial tissues does not seem to reduce RAF inhibitor efficacy and, in fact, allows doubling of the PF-04880594 dose without toxicity usually associated with such doses. These findings indicated that combination treatment with MEK inhibitors might greatly increase the safety and therapeutic index of RAF inhibitors for the treatment of melanoma and other cancers. ©2012 AACR.

  17. Synthesis and preclinical evaluation of [11C-carbonyl]PF-04457845 for neuroimaging of fatty acid amide hydrolase

    International Nuclear Information System (INIS)

    Hicks, Justin W.; Parkes, Jun; Sadovski, Oleg; Tong, Junchao; Houle, Sylvain; Vasdev, Neil; Wilson, Alan A.

    2013-01-01

    Introduction: Fatty acid amide hydrolase (FAAH) has a significant role in regulating endocannabinoid signaling in the central nervous system. As such, FAAH inhibitors are being actively sought for pain, addiction, and other indications. This has led to the recent pursuit of positron emission tomography (PET) radiotracers targeting FAAH. We report herein the preparation and preclinical evaluation of [ 11 C-carbonyl]PF-04457845, an isotopologue of the potent irreversible FAAH inhibitor. Methods: PF-04457845 was radiolabeled at the carbonyl position via automated [ 11 C]CO 2 -fixation. Ex vivo brain biodistribution of [ 11 C-carbonyl]PF-04457845 was carried out in conscious rats. Specificity was determined by pre-administration of PF-04457845 or URB597 prior to [ 11 C-carbonyl]PF-04457845. In a separate experiment, rats injected with the title radiotracer had whole brains excised, homogenized and extracted to examine irreversible binding to brain parenchyma. Results: The title compound was prepared in 5 ± 1% (n = 4) isolated radiochemical yield based on starting [ 11 C]CO 2 (decay uncorrected) within 25 min from end-of-bombardment in > 98% radiochemical purity and a specific activity of 73.5 ± 8.2 GBq/μmol at end-of-synthesis. Uptake of [ 11 C-carbonyl]PF-04457845 into the rat brain was high (range of 1.2–4.4 SUV), heterogeneous, and in accordance with reported FAAH distribution. Saturable binding was demonstrated by a dose-dependent reduction in brain radioactivity uptake following pre-treatment with PF-04457845. Pre-treatment with the prototypical FAAH inhibitor, URB597, reduced the brain radiotracer uptake in all regions by 71–81%, demonstrating specificity for FAAH. The binding of [ 11 C-carbonyl]PF-04457845 to FAAH at 40 min post injection was irreversible as 98% of the radioactivity in the brain could not be extracted. Conclusions: [ 11 C-carbonyl]PF-04457845 was rapidly synthesized via an automated radiosynthesis. Ex vivo biodistribution studies in

  18. Heart failure - tests

    Science.gov (United States)

    CHF - tests; Congestive heart failure - tests; Cardiomyopathy - tests; HF - tests ... the best test to: Identify which type of heart failure (systolic, diastolic, valvular) Monitor your heart failure and ...

  19. An Examination of the Relationship of the 16PF Fifth Edition to a Multidimensional Model of Self-Esteem

    OpenAIRE

    Dale Pietrzak; Betsy Page; James S. Korcuska; Amber Bach Gorman

    2015-01-01

    The regression equation needed to predict the self-esteem score from the Sixteen Personality Factor Questionnaire (16PF) Fifth Edition is not available for hand scoring. This score is derived from the Coopersmith’s Self-Esteem Inventory (SEI), which is based on a unitary model of self-esteem. The SEI may be subject to gender and extroversion biases. Moreover, there is a gap in the literature on the 16PF fifth edition a...

  20. Studies on the thermal decomposition kinetics of LiPF6 and LiBC4O8

    Indian Academy of Sciences (India)

    WINTEC

    ion batteries. The current electrolyte generally used in lithium-ion batteries is mainly composed of LiPF6 and a blend of alkyl carbonates. LiPF6 has been widely adopted for more than a decade, owing to its neces- sary pre-requisites for use in lithium ion batteries, for example, it is easily soluble in various solvents, can lead ...

  1. Potentially functional SNPs (pfSNPs as novel genomic predictors of 5-FU response in metastatic colorectal cancer patients.

    Directory of Open Access Journals (Sweden)

    Jingbo Wang

    Full Text Available 5-Fluorouracil (5-FU and its pro-drug Capecitabine have been widely used in treating colorectal cancer. However, not all patients will respond to the drug, hence there is a need to develop reliable early predictive biomarkers for 5-FU response. Here, we report a novel potentially functional Single Nucleotide Polymorphism (pfSNP approach to identify SNPs that may serve as predictive biomarkers of response to 5-FU in Chinese metastatic colorectal cancer (CRC patients. 1547 pfSNPs and one variable number tandem repeat (VNTR in 139 genes in 5-FU drug (both PK and PD pathway and colorectal cancer disease pathways were examined in 2 groups of CRC patients. Shrinkage of liver metastasis measured by RECIST criteria was used as the clinical end point. Four non-responder-specific pfSNPs were found to account for 37.5% of all non-responders (P<0.0003. Five additional pfSNPs were identified from a multivariate model (AUC under ROC = 0.875 that was applied for all other pfSNPs, excluding the non-responder-specific pfSNPs. These pfSNPs, which can differentiate the other non-responders from responders, mainly reside in tumor suppressor genes or genes implicated in colorectal cancer risk. Hence, a total of 9 novel SNPs with potential functional significance may be able to distinguish non-responders from responders to 5-FU. These pfSNPs may be useful biomarkers for predicting response to 5-FU.

  2. Multiple ETS family proteins regulate PF4 gene expression by binding to the same ETS binding site.

    Directory of Open Access Journals (Sweden)

    Yoshiaki Okada

    Full Text Available In previous studies on the mechanism underlying megakaryocyte-specific gene expression, several ETS motifs were found in each megakaryocyte-specific gene promoter. Although these studies suggested that several ETS family proteins regulate megakaryocyte-specific gene expression, only a few ETS family proteins have been identified. Platelet factor 4 (PF4 is a megakaryocyte-specific gene and its promoter includes multiple ETS motifs. We had previously shown that ETS-1 binds to an ETS motif in the PF4 promoter. However, the functions of the other ETS motifs are still unclear. The goal of this study was to investigate a novel functional ETS motif in the PF4 promoter and identify proteins binding to the motif. In electrophoretic mobility shift assays and a chromatin immunoprecipitation assay, FLI-1, ELF-1, and GABP bound to the -51 ETS site. Expression of FLI-1, ELF-1, and GABP activated the PF4 promoter in HepG2 cells. Mutation of a -51 ETS site attenuated FLI-1-, ELF-1-, and GABP-mediated transactivation of the promoter. siRNA analysis demonstrated that FLI-1, ELF-1, and GABP regulate PF4 gene expression in HEL cells. Among these three proteins, only FLI-1 synergistically activated the promoter with GATA-1. In addition, only FLI-1 expression was increased during megakaryocytic differentiation. Finally, the importance of the -51 ETS site for the activation of the PF4 promoter during physiological megakaryocytic differentiation was confirmed by a novel reporter gene assay using in vitro ES cell differentiation system. Together, these data suggest that FLI-1, ELF-1, and GABP regulate PF4 gene expression through the -51 ETS site in megakaryocytes and implicate the differentiation stage-specific regulation of PF4 gene expression by multiple ETS factors.

  3. Dose-ranging evaluation of intravitreal siRNA PF-04523655 for diabetic macular edema (the DEGAS study)

    DEFF Research Database (Denmark)

    Nguyen, Quan Dong; Schachar, Ronald A; Nduaka, Chudy I

    2012-01-01

    To evaluate the safety and efficacy of three doses of PF-04523655, a 19-nucleotide methylated double stranded siRNA targeting the RTP801 gene, for the treatment of diabetic macular edema (DME) compared to focal/grid laser photocoagulation.......To evaluate the safety and efficacy of three doses of PF-04523655, a 19-nucleotide methylated double stranded siRNA targeting the RTP801 gene, for the treatment of diabetic macular edema (DME) compared to focal/grid laser photocoagulation....

  4. Decrease the Number of Glovebox Glove Breaches and Failures

    Energy Technology Data Exchange (ETDEWEB)

    Hurtle, Jackie C. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2013-12-24

    Los Alamos National Laboratory (LANL) is committed to the protection of the workers, public, and environment while performing work and uses gloveboxes as engineered controls to protect workers from exposure to hazardous materials while performing plutonium operations. Glovebox gloves are a weak link in the engineered controls and are a major cause of radiation contamination events which can result in potential worker exposure and localized contamination making operational areas off-limits and putting programmatic work on hold. Each day of lost opportunity at Technical Area (TA) 55, Plutonium Facility (PF) 4 is estimated at $1.36 million. Between July 2011 and June 2013, TA-55-PF-4 had 65 glovebox glove breaches and failures with an average of 2.7 per month. The glovebox work follows the five step safety process promoted at LANL with a decision diamond interjected for whether or not a glove breach or failure event occurred in the course of performing glovebox work. In the event that no glove breach or failure is detected, there is an additional decision for whether or not contamination is detected. In the event that contamination is detected, the possibility for a glove breach or failure event is revisited.

  5. What Is the Economic Cost of Unplanned Pregnancy Following Hysteroscopic Sterilization in the US? A New National Estimate Based on Essure® Procedure Prevalence, Failure Rates and Workforce Productivity.

    Science.gov (United States)

    Sills, E Scott; Fernandez, Luca P; Jones, Christopher A

    Although hysteroscopic sterilization (HS) (Essure ®) has been available in the US since 2002, there is disagreement regarding its efficacy, and there has been no study of the economic impact of HS failure. Our investigation examined the economic consequences of contraceptive failure with Essure in the US. Contraceptive failure rates (CFR) of 5.7%, 7.7% and 9.6% were applied to the US cohort of HS patients (n = 600,000). Direct economic impact of productivity losses resulting from unplanned conceptions after HS was calculated by factoring Essure failure rate, the exposed population, US female labour force participation, unemployment rate, time away from work owing to vaginal delivery or pregnancy termination and weekly wages. For the 9.6% CFR scenario, US workforce productivity loss from unplanned pregnancy and delivery was estimated at 771,065 days (2,112 years). Productivity loss secondary to conception and subsequent termination of pregnancy after Essure was approximately 23,725 days (65 years). Assuming CFR at 5.7%, livebirth delivery with total time missed from work at 65 days, this was associated with an aggregate economic impact of $49.2M in lost annual wages. Direct economic impact of unplanned pregnancy after Essure irrespective of outcome (terminations and deliveries) was estimated to result in US productivity losses valued at ~$130M. Although not all unplanned pregnancy costs are attributable to failed HS, estimates derived from earlier surveys have not considered this contraceptive method, and the economic consequences of unplanned pregnancy after Essure are not trivial. Quantifying the economic consequences of HS failure would be improved with specific ICD-10 coding for Essure-associated symptoms.

  6. Diagnosis of Equipment Failures by Pattern Recognition

    DEFF Research Database (Denmark)

    Pau, L. F.

    1974-01-01

    The main problems in relation to automatic fault finding and diagnosis in equipments or production systems are discussed: 1) compression of the syndrome and observation spaces for better discrimination between failure modes; 2) simultaneous display of the failure patterns and the failure instants...

  7. Structure of a putative BenF-like porin from Pseudomonas fluorescens Pf-5 at 2.6 A resolution

    Energy Technology Data Exchange (ETDEWEB)

    Sampathkumar, P.; Swaminathan, S.; Lu, F.; Zhao, X.; Li, Z.; Gilmore, J.; Bain, K.; Rutter, M. E.; Gheyi, T.; Schwinn, D.; Bonanno, J. B.; Pieper, U.; Fajardo, J. E.; Fiser, A.; Almo, S. C.; Chance, M. R.; Baker, D.; Atwell, S.; Thompson, D. A.; Emtage, J. S.; Wasserman, S. R.; Sali, A.; Sauder, J. M.; Burley, S. K.

    2010-11-01

    of benzoate. BLAST analysis of the amino acid sequence of P. fluorescens Pf-5 gene PFL1329 (Uniprot id: http://www.uniprot.org/uniprot/Q4KH25) against P. putida KT2440 strain identified 20 related porins. The top six hits include P. putida KT2440 genes PP1383 (annotated as BenF-like), PP2517 (annotated as BenF-like), and PP3168 (annotated as BenF), which share sequence identities of 76%, 66%, and 44% with PFL1329, respectively. The precise functions of these genes are not yet known. Therefore, we refer to the protein product of gene PFL1329 as PflBenF-like, which reflects its current annotation in the Uniprot database. Crystal structures of OprD and OpdK (vanillate specific porin), both from P. aeruginosa (designated below as PaOprD and PaOpdK, respectively) have been determined. Herein, we report the crystal structure of a putative BenF-like porin from P. fluorescens Pf-5 (PflBenF-like). For the sake of brevity, all subsequent references to the PflBenF-like porin will be made using PflBenF. X-ray crystallography revealed a canonical 18-stranded {beta}-barrel fold that forms a central pore with a diameter of {approx}4.6 E. We describe detailed comparisons of the PflBenF structure with those of PaOprD and PaOpdK.

  8. Heart failure - home monitoring

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000113.htm Heart failure - home monitoring To use the sharing features on ... your high blood pressure Fast food tips Heart failure - discharge Heart failure - fluids and diuretics Heart failure - what to ...

  9. Measurements of fusion-protons anisotropy around the pinch axis within high-current PF-1000 experiments

    Energy Technology Data Exchange (ETDEWEB)

    Sadowski, M.J. [The Andrzej Soltan Institute for Nuclear Studies - IPJ, 05-400 Otwock-Swierk (Poland)] [Institute of Plasma Physics and Laser Microfusion - IPPLM, 01-497 Warsaw (Poland); Malinowska, A.; Malinowski, K.; Czaus, K.; Kwiatkowski, R.; Skladnik-Sadowska, E.; Zebrowski, J. [The Andrzej Soltan Institute for Nuclear Studies - IPJ, 05-400 Otwock-Swierk (Poland); Karpinski, L.; Paduch, M.; Scholz, M.; Stepniewski, W. [Institute of Plasma Physics and Laser Microfusion - IPPLM, 01-497 Warsaw (Poland)

    2011-07-01

    The paper describes measurements of fast protons produced by D-D fusion reactions during high-current discharges within the PF-1000 facility operated with the deuterium filling at 27 kV, 480 kJ. The measurements were performed by means of a set of pinhole-cameras equipped with PM-355 track detectors shielded by 80-{mu}m-thick Al-filters, which eliminated fast primary deuterons and protons of lower energy (< 3 MeV). Those cameras were placed at different angles around the pinch axis. The obtained proton images showed a distinct angular anisotropy, which was explained by an influence of local magnetic fields connected with a filamentary structure of the plasma column during the fast proton (and neutron) emission. The paper shows that in addition to measurements of a fusion neutron anisotropy it is reasonable to study also an anisotropy of fusion protons (originated from the second branch of the D-D reactions), as well as other charged fusion products. This document is composed of a paper followed by a poster

  10. The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1

    Energy Technology Data Exchange (ETDEWEB)

    Shakya, Bikash; Penn, Wesley D.; Nakayasu, Ernesto S.; Lacount, Douglas J.

    2017-09-01

    Plasmodium falciparum extensively modifies the infected red blood cell (RBC), resulting in changes in deformability, shape and surface properties. These alterations suggest that the RBC cytoskeleton is a major target for modification during infection. However, the molecular mechanisms leading to these changes are largely unknown. To begin to address this question, we screened for exported P. falciparum proteins that bound to the erythrocyte cytoskeleton proteins ankyrin 1 (ANK1) and band 4.1 (4.1R), which form critical interactions with other cytoskeletal proteins that contribute to the deformability and stability of RBCs. Yeast two-hybrid screens with ANK1 and 4.1R identified eight interactions with P. falciparum exported proteins, including an interaction between 4.1R and PF3D7_0402000 (PFD0090c). This interaction was first identified in a large-scale screen (Vignali et al., Malaria J, 7:211, 2008), which also reported an interaction between PF3D7_0402000 and ANK1. We confirmed the interactions of PF3D7_0402000 with 4.1R and ANK1 in pair-wise yeast two-hybrid and co-precipitation assays. In both cases, an intact PHIST domain in PF3D7_0402000 was required for binding. Complex purification followed by mass spectrometry analysis provided additional support for the interaction of PF3D7_0402000 with ANK1 and 4.1R. RBC ghost cells loaded with maltose-binding protein (MBP)-PF3D7_0402000 passed through a metal microsphere column less efficiently than mock- or MBP-loaded controls, consistent with an effect of PF3D7_0402000 on RBC rigidity or membrane stability. This study confirmed the interaction of PF3D7_0402000 with 4.1R in multiple independent assays, provided the first evidence that PF3D7_0402000 also binds to ANK1, and suggested that PF3D7_0402000 affects deformability or membrane stability of uninfected RBC ghosts.

  11. Evaluation of fuel-temperature feedback mechanisms in TRAC-PF1/MOD2/NESTLE

    International Nuclear Information System (INIS)

    Knepper, Paula L.; Feltus, Madeline; Hochreiter, L.E.; Ivanov, Kostadin

    1999-01-01

    Coupled spatial kinetics and thermal-hydraulics system codes provide a means to model transient nuclear reactor behavior more accurately. Transients marked by strong perturbations, both with thermal-hydraulics and neutronics, such as a control-rod ejection or a main steam-line break, are especially of interest. It is now feasible to model complex reactor behavior with a coupled thermal-hydraulics and spatial kinetics code that provides a means to forecast safety margins. Recently, the Transient Reactor Analysis Code (TRAC)-PF1/MOD2, Version 5.4.25, was coupled with the NESTLE code. This coupled code (TRAC-PF1/MOD2/NESTLE) is used to examine effective fuel-temperature models. The Electric Power Research Institute (EPRI) rod-ejection benchmark was analyzed to evaluate the influence of effective fuel temperature. The rod-ejection transient tests only the fuel-rod, heat-conduction coupling. The coolant thermal-hydraulic coupling is not tested because of the speed of the transient. The neutronics solution changes extremely rapidly, whereas the convective heat transfer at the fuel surface requires more time to influence the coolant temperature of the system. The need to model the response of the system coolant temperature is not crucial in this analysis. The influence of the effective fuel temperature is the key component of this study. Various models were examined using the coupled code to calculate effective fuel temperatures. The influence of different, effective fuel-temperature models on the coupled-code results is studied. Three effective fuel-temperature models are examined: (l) volume average effective fuel temperature, (2) the effective fuel-temperature model suggested by the Office of Economic Cooperation and Development (OECD) rod-ejection benchmark, and (3) the NESTLE effective fuel-temperature model. A discussion is provided describing the effective fuel-temperature models examined in TRAC-PF1/MOD2/NESTLE and the influence of effective fuel temperature in

  12. Glass transition in the spin-density wave phase of (TMTSF)2PF6

    DEFF Research Database (Denmark)

    Lasjaunias, J.C.; Biljakovic, K.; Nad, F.

    1994-01-01

    We present the results of low frequency dielectric measurements and a detailed kinetic investigation of the specific heat anomaly in the spin-density wave phase of (TMTSF)(2)PF6 in the temperature range between 2 and 4 K. The dielectric relaxation shows a critical slowing down towards a ''static'......'' glass transition around 2 K. The jump in the specific heat in different controlled kinetic conditions shows all the characteristics of freezing in supercooled liquids. Both effects give direct evidence of a glass transition in the spin-density wave ground state....

  13. Current Sharing Analysis of Arm Prototype for ITER PF Converter Bridge

    International Nuclear Information System (INIS)

    Li Jinchao; Song Zhiquan; Xu Liuwei; Fu Peng; Guo Bin; Li Sen; Dong Lin; Wang Min

    2014-01-01

    A bridge arm prototype of ITER poloidal field (PF) converter modules has been designed and fabricated. Non-cophase counter parallel connection is chosen as the arm structure of the prototype. Among all factors affecting current sharing, arm structure is the main one. During the design of the arm prototype, a novel method based on inductance matrixes is employed to improve the current sharing of the bridge arm. The test results on the prototype show that the current sharing performance of the arm prototype is much better than relevant design requirement, and that the matrix method is very effective to analyze and solve the current sharing problems of thyristor converters

  14. Suppression of Longitudinal Coupled-Bunch Instabilities at the KEK-PF

    International Nuclear Information System (INIS)

    Obina, T.; Tobiyama, M.; Honda, T.; Tadano, M.; Flanagan, J.W.; Mitsuhashi, T.; Cheng, W.X.; Fox, J.D.; Teytelman, D.

    2012-01-01

    A bunch-by-bunch feedback system has been developed to suppress longitudinal coupled-bunch instabilities at the KEK-PF. A longitudinal kicker based on a DAFNE-type overdamped cavity has been designed and installed in the ring, and a general purpose signal processor, called iGp, has been developed by the collaboration of the KEK, SLAC, and INFN-LNF. The entire feedback loop has been closed by the end of June 2007, and the feedback system has successfully suppressed the longitudinal dipole-mode instabilities up to 430 mA.

  15. Revisión sistemática del Cuestionario Factorial de Personalidad (16PF)

    OpenAIRE

    Sánchez-Gallego, Nancy Janeth; Gómez-Macías, Carolina; Zambrano-Cruz, Renato

    2011-01-01

    Este artículo busca establecer el uso e impacto de la prueba de personalidad 16PF en publicaciones académicas durante el periodo 2000-2010. Para lograr dicho objetivo en esta investigación documental se utilizó la metodología de revisión sistemática de literatura en la que se encontraron 30 artículos que cumplen con los criterios de inclusión. Se observa que la prueba ha sido utilizada en diversas investigaciones alrededor del mundo de manera constante,aunque se discute el uso de la cuarta ed...

  16. Validity study of the 16PF in terminal air traffic control work

    International Nuclear Information System (INIS)

    Karson, S.; O'Dell, J.W.

    1976-01-01

    Personality test scores obtained in 1966 from the 16PF, Form A are compared with supervisory ratings on overall job ability made at least two years later on a sample of 510 terminal area journeymen controllers. Those rated above average were found to differ significantly from those rated below average in ability on one primary personality factor, namely, C (ego strength). Other factors which approached significance are also discussed. The present study represents an attempt to determine whether personality factor scores are valid over longer periods of time in successfully discriminating journeymen terminal controllers rated above and below average in overall ability by their supervisors

  17. Recent studies of the ion emission from high-current PF-1000 experiments

    International Nuclear Information System (INIS)

    Skladnik-Sadowska, E.; Sadowski, M.J.; Czaus, K.; Malinowski, K.; Kwiatkowski, R.; Zebrowski, J.; Sadowski, M.J.; Karpinski, L.; Paduch, M.; Scholz, M.; Garkusha, I.E.

    2010-01-01

    The paper describes diagnostics of fast ion beams emitted from a large PF-1000 facility operated at 21...27 kV, 290...480 kJ. Those beams were measured with pinhole cameras and PM-355 nuclear track detectors, placed at different angles to the discharge axis. The measurements showed a complex spatial structure of the fast ion beams. Measurements behind an axial channel in the inner electrode have shown that some ion beams are emitted also in the upstream direction. The ion energy spectra were measured with a miniature Thomson-type spectrometer. Time-resolved measurements of ions were performed with miniature scintillation detectors placed behind a pinhole.

  18. Vibrational autoionization in PF3: Doing violence to the propensity rule

    International Nuclear Information System (INIS)

    Berkowitz, J.; Greene, J.P.

    1984-01-01

    The photoionization spectrum of PF + 3 in its threshold region displays two prominent progressions of autoionization peaks. When these are analyzed, together with earlier photoabsorption studies and a photoelectron spectrum, they lead to the conclusion that vibrational autoionization is occurring, with Δν< or =-13. This conclusion stands in sharp contrast with the current theory of vibrational autoionization, which predicts a propensity rule Δν = -1. Other examples from the recent literature are summarized, to suggest that a more general theory of vibrational autoionization is required

  19. PF-4708671, a specific inhibitor of p70 ribosomal S6 kinase 1, activates Nrf2 by promoting p62-dependent autophagic degradation of Keap1

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jeong Su [Severance Biomedical Science Institute (Korea, Republic of); Yonsei Biomedical Research Institute, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752 (Korea, Republic of); Kang, Dong Hoon [Department of Life Science and Ewha Research Center for Systems Biology (Korea, Republic of); The Research Center for Cell Homeostasis, Ewha Womans University, Seoul 127-750 (Korea, Republic of); Lee, Da Hyun [Severance Biomedical Science Institute (Korea, Republic of); Yonsei Biomedical Research Institute, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752 (Korea, Republic of); Bae, Soo Han, E-mail: soohanbae@yuhs.ac [Severance Biomedical Science Institute (Korea, Republic of); Yonsei Biomedical Research Institute, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752 (Korea, Republic of)

    2015-10-23

    p70 ribosomal S6 kinase 1 (S6K1) is an important serine/threonine kinase and downstream target of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway. PF-4708671 is a specific inhibitor of S6K1, and prevents S6K1-mediated phosphorylation of the S6 protein. PF-4708671 treatment often leads to apoptotic cell death. However, the protective mechanism against PF-4708671-induced cell death has not been elucidated. The nuclear factor erythroid 2-related factor 2 (Nrf2)-Kelch-like ECH-associated protein 1 (Keap1) pathway is essential for protecting cells against oxidative stress. p62, an adaptor protein in the autophagic process, enhances Nrf2 activation through the impairment of Keap1 activity. In this study, we showed that PF-4708671 induces autophagic Keap1 degradation-mediated Nrf2 activation in p62-dependent manner. Furthermore, p62-dependent Nrf2 activation plays a crucial role in protecting cells from PF-4708671-mediated apoptosis. - Highlights: • PF-4708671, a S6K1-specific inhibitor, prevents S6K1-mediated S6 phosphorylation. • However, PF-4708671 treatment often leads to apoptotic cell death. • Protective mechanism against PF-4708671-induced cell death remains to be elucidated. • PF-4708671 induced p62-dependent, autophagic Keap1 degradation-mediated Nrf2 activation. • p62-dependent Nrf2 activation protects cells from PF-4708671-mediated apoptosis.

  20. A method for visualizing surface-exposed and internal PfEMP1 adhesion antigens in Plasmodium falciparum infected erythrocytes

    Directory of Open Access Journals (Sweden)

    Arnot David E

    2008-06-01

    Full Text Available Abstract Background The insertion of parasite antigens into the host erythrocyte membrane and the structure and distribution of Plasmodium falciparum adhesion receptors on that membrane are poorly understood. Laser scanning confocal microscopy (LSCM and a novel labelling and fixation method have been used to obtain high resolution immuno-fluorescent images of erythrocyte surface PfEMP1 and internal antigens which allow analysis of the accumulation of PfEMP1 on the erythrocyte membrane during asexual development. Methods A novel staining technique has been developed which permits distinction between erythrocyte surface PfEMP1 and intracellular PfEMP1, in parasites whose nuclear material is exceptionally well resolved. Primary antibody detection by fluorescence is carried out on the live parasitized erythrocyte. The surface labelled cells are then fixed using paraformaldehyde and permeabilized with a non-ionic detergent to permit access of antibodies to internal parasite antigens. Differentiation between surface and internal antigens is achieved using antibodies labelled with different fluorochromes and confocal microscopy Results Surface exposed PfEMP1 is first detectable by antibodies at the trophozoite stage of intracellular parasite development although the improved detection method indicates that there are differences between different laboratory isolates in the kinetics of accumulation of surface-exposed PfEMP1. Conclusion A sensitive method for labelling surface and internal PfEMP1 with up to three different fluorochromes has been developed for laser scanning confocal optical microscopy and the analysis of the developmental expression of malaria adhesion antigens.

  1. Transcript and protein expression profile of PF11_0394, a Plasmodium falciparum protein expressed in salivary gland sporozoites

    Directory of Open Access Journals (Sweden)

    Schlarman Maggie S

    2012-03-01

    Full Text Available Abstract Background Plasmodium falciparum malaria is a significant problem around the world today, thus there is still a need for new control methods to be developed. Because the sporozoite displays dual infectivity for both the mosquito salivary glands and vertebrate host tissue, it is a good target for vaccine development. Methods The P. falciparum gene, PF11_0394, was chosen as a candidate for study due to its potential role in the invasion of host tissues. This gene, which was selected using a data mining approach from PlasmoDB, is expressed both at the transcriptional and protein levels in sporozoites and likely encodes a putative surface protein. Using reverse transcription-polymerase chain reaction (RT-PCR and green fluorescent protein (GFP-trafficking studies, a transcript and protein expression profile of PF11_0394 was determined. Results The PF11_0394 protein has orthologs in other Plasmodium species and Apicomplexans, but none outside of the group Apicomplexa. PF11_0394 transcript was found to be present during both the sporozoite and erythrocytic stages of the parasite life cycle, but no transcript was detected during axenic exoerythrocytic stages. Despite the presence of transcript throughout several life cycle stages, the PF11_0394 protein was only detected in salivary gland sporozoites. Conclusions PF11_0394 appears to be a protein uniquely detected in salivary gland sporozoites. Even though a specific function of PF11_0394 has not been determined in P. falciparum biology, it could be another candidate for a new vaccine.

  2. Plasmodium falciparum Hop (PfHop Interacts with the Hsp70 Chaperone in a Nucleotide-Dependent Fashion and Exhibits Ligand Selectivity.

    Directory of Open Access Journals (Sweden)

    Tawanda Zininga

    Full Text Available Heat shock proteins (Hsps play an important role in the development and pathogenicity of malaria parasites. One of the most prominent functions of Hsps is to facilitate the folding of other proteins. Hsps are thought to play a crucial role when malaria parasites invade their host cells and during their subsequent development in hepatocytes and red blood cells. It is thought that Hsps maintain proteostasis under the unfavourable conditions that malaria parasites encounter in the host environment. Although heat shock protein 70 (Hsp70 is capable of independent folding of some proteins, its functional cooperation with heat shock protein 90 (Hsp90 facilitates folding of some proteins such as kinases and steroid hormone receptors into their fully functional forms. The cooperation of Hsp70 and Hsp90 occurs through an adaptor protein called Hsp70-Hsp90 organising protein (Hop. We previously characterised the Hop protein from Plasmodium falciparum (PfHop. We observed that the protein co-localised with the cytosol-localised chaperones, PfHsp70-1 and PfHsp90 at the blood stages of the malaria parasite. In the current study, we demonstrated that PfHop is a stress-inducible protein. We further explored the direct interaction between PfHop and PfHsp70-1 using far Western and surface plasmon resonance (SPR analyses. The interaction of the two proteins was further validated by co-immunoprecipitation studies. We observed that PfHop and PfHsp70-1 associate in the absence and presence of either ATP or ADP. However, ADP appears to promote the association of the two proteins better than ATP. In addition, we investigated the specific interaction between PfHop TPR subdomains and PfHsp70-1/ PfHsp90, using a split-GFP approach. This method allowed us to observe that TPR1 and TPR2B subdomains of PfHop bind preferentially to the C-terminus of PfHsp70-1 compared to PfHsp90. Conversely, the TPR2A motif preferentially interacted with the C-terminus of PfHsp90. Finally, we

  3. On the Quality of Failure

    Directory of Open Access Journals (Sweden)

    Bengt-Arne Vedin

    2014-05-01

    Full Text Available No innovation without failed experiments. No acquisition of competency without hard work, efforts to translate mistakes and insufficient – failed – attempts into mastery. The human condition is one defined by trial and error. Success equates with narrow escape from failure, whereas failure may be a success just missed. The purpose of management can be said to achieve success, so the management of failure is a key one, seeking to turn failure into profit. Almost no successful solutions remain eternally, so signals indicating that they have arrived in their twilight zone are important. Success may be too comforting and lulling, creating a dangerous success lock-in. Courting failure by acting against conventional wisdom and routine intuitions may, though risky, generate huge payoffs. A productive failure management should appreciate that failure is a way to uncertainty reduction, to better information and knowledge, new wisdom and new intuitions. Failure can and should be celebrated and harvested. The most successful mineral prospectors are those who drill more holes; while their percentage of dry ones is average, their number of lucky ones makes for success. The same holds for ideas, where there is the additional benefit that a larger number of ideas, diverse as they are, makes for improved idea handling proficiency.

  4. [A place in the macrocosmos: Paracelsus in Bad Pfäfers].

    Science.gov (United States)

    Pörksen, G

    1994-03-29

    The script about Bad Pfäfers is an exception within the entire work of Paracelsus: It is short, completely worked over and has been printed already during his lifetime. It contains an unique list of comments on latin termini in his native language, hints about the context of their origin and may be read like a concentrate of his philosophy. Biography and personality of Paracelsus are briefly outlined; thereafter, the dark preface of the script on Bad Pfäfers is commented on. The latter turned out to be a stenograph of his central ideas on natural philosophy and cosmology as expressed in other scripts of the thirties, in the 'Astronomia magna' in particular (1536). The concept of the human body, of his macrocosmic derivation and of the 'source' as natural testimony of the healing nature of Christ becomes clear. Finally, from circumstantial evidence in text and context of the script, it is concluded, that it must have derived from the dialogue with the personnel of the spa uneducated in a humanistic sense, and may be understood as an abbreviated draft ("aide-mémoire") of Paracelsic instructions.

  5. Pf1 bacteriophage hydration by magic angle spinning solid-state NMR

    Energy Technology Data Exchange (ETDEWEB)

    Sergeyev, Ivan V.; Bahri, Salima; McDermott, Ann E., E-mail: aem5@columbia.edu [Department of Chemistry, Columbia University, 3000 Broadway, New York, New York 10027 (United States); Day, Loren A. [Public Health Research Institute, Rutgers University, 225 Warren St., Newark, New Jersey 07103 (United States)

    2014-12-14

    High resolution two- and three-dimensional heteronuclear correlation spectroscopy ({sup 1}H–{sup 13}C, {sup 1}H–{sup 15}N, and {sup 1}H–{sup 13}C–{sup 13}C HETCOR) has provided a detailed characterization of the internal and external hydration water of the Pf1 virion. This long and slender virion (2000 nm × 7 nm) contains highly stretched DNA within a capsid of small protein subunits, each only 46 amino acid residues. HETCOR cross-peaks have been unambiguously assigned to 25 amino acids, including most external residues 1–21 as well as residues 39–40 and 43–46 deep inside the virion. In addition, the deoxyribose rings of the DNA near the virion axis are in contact with water. The sets of cross-peaks to the DNA and to all 25 amino acid residues were from the same hydration water {sup 1}H resonance; some of the assigned residues do not have exchangeable side-chain protons. A mapping of the contacts onto structural models indicates the presence of water “tunnels” through a highly hydrophobic region of the capsid. The present results significantly extend and modify results from a lower resolution study, and yield a comprehensive hydration surface map of Pf1. In addition, the internal water could be distinguished from external hydration water by means of paramagnetic relaxation enhancement. The internal water population may serve as a conveniently localized magnetization reservoir for structural studies.

  6. Tumor p-glycoprotein correlates with efficacy of PF-3758309 in in vitro and in vivo models of colorectal cancer.

    Directory of Open Access Journals (Sweden)

    Erica Lynn Bradshaw-Pierce

    2013-03-01

    Full Text Available P-glycoprotein (P-gp, a member of the ATP-binding cassette transporter family, is overexpressed in a number of different cancers and some studies show that P-gp overexpression can be correlated to poor prognosis or therapeutic resistance. Here we sought to elucidate if PF-3758309 (PF-309, a novel p-21 activated kinase inhibitor, efficacy was influenced by tumor P-gp. Based on in vitro proliferation data, a panel of colorectal cancer cell lines were ranked as sensitive or resistant and ABCB1 (P-gp expression was evaluated by microarray for these cell lines. P-gp expression was determined by western blot and activity determined by rhodamine efflux assay. Knock down of P-gp and pharmacologic inhibition of P-gp to restore PF-309 activity was performed in vitro. PF-309 activity was evaluated in vivo in cell line xenograft models and in primary patient derived tumor xenografts (PDTX. Mice were treated with 25 mg/kg PF-309 orally, twice daily. On the last day of treatment, tumor and plasma were collected for PF-309 analysis. Here we show that ABCB1 gene expression correlates with resistance to PF-309 treatment in vitro and the expression and activity of P-gp was verified in a panel of resistant cells. Furthermore, inhibition of P-gp increased the sensitivity of resistant cells, resulting in a 4-100 fold decrease in the IC50s. Eleven cell line xenografts and 12 PDTX models were treated with PF-309. From the cell line xenografts, we found a significant correlation between ABCB1 gene expression profiles and tumor response. We evaluated tumor and plasma concentrations for 8 tumor models (3 cell line xenografts and 5 PDTX models and a significant correlation was found between tumor concentration and response. Additionally, we show that tumor concentration is approximately 4-fold lower in tumors that express P-gp, verified by western blot. Our in vitro and in vivo data strongly suggests that PF-309 efficacy is influenced by the expression of tumor P-gp.

  7. Surface chemistry of PH 3, PF 3 and PCl 3 on Ru(0001)

    Science.gov (United States)

    Tao, H.-S.; Diebold, U.; Shinn, N. D.; Madey, T. E.

    1994-06-01

    The adsorption, desorption and decomposition of PH 3, PF 3 and PCl 3 on Ru(0001) have been studied by soft X-ray photoelectron spectroscopy (SXPS) using synchrotron radiation. Due to large chemical shifts in the P 2p core levels, different phosphorus containing surface species can be identified. We find that PF 3 adsorbs molecularly on Ru(0001) at 80 and 300 K. At 80 K, PH 3 saturates the surface with one layer of atomic hydrogen, elemental phosphorus, subhydride (i.e., PHx (0 PH 3, with a total phosphorus coverage of 0.4 ML. At 300 K, PH 3 decomposes into atomic hydrogen and elemental phosphorus with a phosphorus coverage of 0.8 ML. At 80 K, PCl 3 adsorbs dissociatively into atomic chlorine, elemental phosphorus, PCl and possibly PCl 2 and PCl 3 in the first monolayer. Formation of multilayers of PCl 3 is observed at 80 K. At 300 K, PCl 3 adsorbs dissociatively as atomic chlorine and elemental phosphorus with a saturation phosphorus coverage of 0.1 ML. The variation in total phosphorus uptake at 300 K from PX3 ( X = H, FandCl) adsorption is a result of competition between site blocking by dissociation fragments and displacement reactions. Annealing surfaces with adsorbed phosphorus to 1000 K results in formation of RuzP ( z = 1 or 2), which is manifested by the chemical shifts in the P2p core level, as well as the P LVV Auger transition. The recombination of adsorbed phosphorus and adsorbed X ( = H, FandCl) from decomposition is also observed, but is a minor reaction channel on the surface. Thermochemical data are used to analyze the different stabilities of PX 3 at 300 K, namely, PF 3 adsorbs molecularly and PH 3 and PCl 3 dissociate completely. First, we compare the heat of molecular adsorption and the heat of dissociative adsorption of PX 3 on Ru(0001), using an enthalpy approach, and find results consistent with experimental observations. Second, we compare the total bond energy difference between molecular adsorption and complete dissociation of PX 3 on Ru

  8. Memory decline in Down syndrome and its relationship to iPF2alpha, a urinary marker of oxidative stress.

    Directory of Open Access Journals (Sweden)

    Panagiotis Zis

    Full Text Available Lipid peroxidation may be a marker of free-radical-mediated injury associated with Alzheimer's disease (AD. We aimed to investigate whether changes in lipid peroxidation is associated with cognitive decline in individuals with Down syndrome over a 4-year period.Thirty-two adults with DS participated in a longitudinal study with urinary isoprostane 8,12-iso-iPF2alpha (iPF2alpha assays at baseline and four years follow-up. Informants rated their functional ability and memory function and the adults with DS attempted assessments of language skills and memory. Twenty-six individuals completed assessments of memory (Modified Memory Object Task, MOMT, adaptive behavior (ABAS, and receptive vocabulary (British Picture vocabulary, BPVS at both time-points.Overall change in iPF2alpha level was negatively correlated with change in the MOMT score (Spearman's Rho =  -0.576, p = 0.006, i.e., increased lipid peroxidation was correlated with worse memory functioning over time. An increase of ≥ 0.02 ng/mg creatinine iPF2α had good sensitivity (85.7%, positive predictive value (75%,, specificity (85.7% and negative predictive value (92.3% for memory decline.Change in iPF2alpha over time may have potential as a biomarker for memory decline in Down syndrome and potentially also help to track progression of MCI to AD in the general population.

  9. Molecular interaction of selected phytochemicals under the charged environment of Plasmodium falciparum chloroquine resistance transporter (PfCRT) model.

    Science.gov (United States)

    Patel, Saumya K; Khedkar, Vijay M; Jha, Prakash C; Jasrai, Yogesh T; Pandya, Himanshu A; George, Linz-Buoy; Highland, Hyacinth N; Skelton, Adam A

    2016-01-01

    Phytochemicals of Catharanthus roseus Linn. and Tylophora indica have been known for their inhibition of malarial parasite, Plasmodium falciparum in cell culture. Resistance to chloroquine (CQ), a widely used antimalarial drug, is due to the CQ resistance transporter (CRT) system. The present study deals with computational modeling of Plasmodium falciparum chloroquine resistance transporter (PfCRT) protein and development of charged environment to mimic a condition of resistance. The model of PfCRT was developed using Protein homology/analogy engine (PHYRE ver 0.2) and was validated based on the results obtained using PSI-PRED. Subsequently, molecular interactions of selected phytochemicals extracted from C. roseus Linn. and T. indica were studied using multiple-iterated genetic algorithm-based docking protocol in order to investigate the translocation of these legends across the PfCRT protein. Further, molecular dynamics studies exhibiting interaction energy estimates of these compounds within the active site of the protein showed that compounds are more selective toward PfCRT. Clusters of conformations with the free energy of binding were estimated which clearly demonstrated the potential channel and by this means the translocation across the PfCRT is anticipated.

  10. The ALK inhibitor PF-06463922 is effective as a single agent in neuroblastoma driven by expression of ALK and MYCN

    Directory of Open Access Journals (Sweden)

    J. Guan

    2016-09-01

    Full Text Available The first-in-class inhibitor of ALK, c-MET and ROS1, crizotinib (Xalkori, has shown remarkable clinical efficacy in treatment of ALK-positive non-small cell lung cancer. However, in neuroblastoma, activating mutations in the ALK kinase domain are typically refractory to crizotinib treatment, highlighting the need for more potent inhibitors. The next-generation ALK inhibitor PF-06463922 is predicted to exhibit increased affinity for ALK mutants prevalent in neuroblastoma. We examined PF-06463922 activity in ALK-driven neuroblastoma models in vitro and in vivo. In vitro kinase assays and cell-based experiments examining ALK mutations of increasing potency show that PF-06463922 is an effective inhibitor of ALK with greater activity towards ALK neuroblastoma mutants. In contrast to crizotinib, single agent administration of PF-06463922 caused dramatic tumor inhibition in both subcutaneous and orthotopic xenografts as well as a mouse model of high-risk neuroblastoma driven by Th-ALKF1174L/MYCN. Taken together, our results suggest PF-06463922 is a potent inhibitor of crizotinib-resistant ALK mutations, and highlights an important new treatment option for neuroblastoma patients.

  11. Biochemical responses and DNA damage in earthworms (Eisenia fetida) induced by ionic liquid [omim]PF6.

    Science.gov (United States)

    Liu, Xiaoyan; Zhang, Shumin; Wang, Jinhua; Wang, Jun; Shao, Yuting; Zhu, Lusheng

    2016-04-01

    Ionic liquids that are not that "green" to many organisms have recently been identified. This study examined the subchronic toxicity of the ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate ([omim]PF6) to earthworms (Eisenia fetida). Earthworms were exposed for a 28-day period (sampled on days 7, 14, 21, and 28) at concentrations of 0, 5, 10, 20, and 40 mg/kg. The levels of reactive oxygen species (ROS), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD)), detoxifying enzyme (glutathione S-transferase (GST)), lipid peroxidation, and DNA damage were measured. ROS significantly accumulated in all the treatment groups; the maximum ROS content was 51.9% higher than the control at 40 mg/kg [omim]PF6 on day 28. Increased SOD activities attenuated over the time of exposure, while the CAT activities of the treatment groups were similar to the controls, except on day 14. Furthermore, the activities of POD and GST were stimulated. Lipid peroxidation in earthworms was not apparent at 5 and 10 mg/kg [omim]PF6 but was quite obvious at 40 mg/kg [omim]PF6. In addition, DNA damage was dose- and time-dependent. In conclusion, [omim]PF6 caused oxidative stress and genotoxicity in earthworms.

  12. Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism

    International Nuclear Information System (INIS)

    Chiu, Hsiu-Ju; Bakolitsa, Constantina; Skerra, Arne; Lomize, Andrei; Carlton, Dennis; Miller, Mitchell D.; Krishna, S. Sri; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Clayton, Thomas; Deller, Marc C.; Duan, Lian; Feuerhelm, Julie; Grant, Joanna C.; Grzechnik, Slawomir K.; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Kozbial, Piotr; Kumar, Abhinav; Marciano, David; McMullan, Daniel; Morse, Andrew T.; Nigoghossian, Edward; Okach, Linda; Paulsen, Jessica; Reyes, Ron; Rife, Christopher L.; Bedem, Henry van den; Weekes, Dana; Xu, Qingping; Hodgson, Keith O.; Wooley, John; Elsliger, Marc-André; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.

    2009-01-01

    NE1406, the first structural representative of PF09410, reveals a lipocalin-like fold with features that suggest involvement in lipid metabolism. In addition, NE1406 provides potential structural templates for two other protein families (PF07143 and PF08622). The first structural representative of the domain of unknown function DUF2006 family, also known as Pfam family PF09410, comprises a lipocalin-like fold with domain duplication. The finding of the calycin signature in the N-terminal domain, combined with remote sequence similarity to two other protein families (PF07143 and PF08622) implicated in isoprenoid metabolism and the oxidative stress response, support an involvement in lipid metabolism. Clusters of conserved residues that interact with ligand mimetics suggest that the binding and regulation sites map to the N-terminal domain and to the interdomain interface, respectively

  13. Numerical estimation on balance coefficients of central difference averaging method for quench detection of the KSTAR PF coils

    International Nuclear Information System (INIS)

    Kim, Jin Sub; An, Seok Chan; Ko, Tae Kuk; Chu, Yong

    2016-01-01

    A quench detection system of KSTAR Poloidal Field (PF) coils is inevitable for stable operation because normal zone generates overheating during quench occurrence. Recently, new voltage quench detection method, combination of Central Difference Averaging (CDA) and Mutual Inductance Compensation (MIK) for compensating mutual inductive voltage more effectively than conventional voltage detection method, has been suggested and studied. For better performance of mutual induction cancellation by adjacent coils of CDA+MIK method for KSTAR coil system, balance coefficients of CDA must be estimated and adjusted preferentially. In this paper, the balance coefficients of CDA for KSTAR PF coils were numerically estimated. The estimated result was adopted and tested by using simulation. The CDA method adopting balance coefficients effectively eliminated mutual inductive voltage, and also it is expected to improve performance of CDA+MIK method for quench detection of KSTAR PF coils

  14. The Malarial Exported PFA0660w Is an Hsp40 Co-Chaperone of PfHsp70-x.

    Directory of Open Access Journals (Sweden)

    Michael O Daniyan

    Full Text Available Plasmodium falciparum, the human pathogen responsible for the most dangerous malaria infection, survives and develops in mature erythrocytes through the export of proteins needed for remodelling of the host cell. Molecular chaperones of the heat shock protein (Hsp family are prominent members of the exportome, including a number of Hsp40s and a Hsp70. PFA0660w, a type II Hsp40, has been shown to be exported and possibly form a complex with PfHsp70-x in the infected erythrocyte cytosol. However, the chaperone properties of PFA0660w and its interaction with human and parasite Hsp70s are yet to be investigated. Recombinant PFA0660w was found to exist as a monomer in solution, and was able to significantly stimulate the ATPase activity of PfHsp70-x but not that of a second plasmodial Hsp70 (PfHsp70-1 or a human Hsp70 (HSPA1A, indicating a potential specific functional partnership with PfHsp70-x. Protein binding studies in the presence and absence of ATP suggested that the interaction of PFA0660w with PfHsp70-x most likely represented a co-chaperone/chaperone interaction. Also, PFA0660w alone produced a concentration-dependent suppression of rhodanese aggregation, demonstrating its chaperone properties. Overall, we have provided the first biochemical evidence for the possible role of PFA0660w as a chaperone and as co-chaperone of PfHsp70-x. We propose that these chaperones boost the chaperone power of the infected erythrocyte, enabling successful protein trafficking and folding, and thereby making a fundamental contribution to the pathology of malaria.

  15. PF2fit: Polar Fast Fourier Matched Alignment of Atomistic Structures with 3D Electron Microscopy Maps.

    Directory of Open Access Journals (Sweden)

    Radhakrishna Bettadapura

    2015-10-01

    Full Text Available There continue to be increasing occurrences of both atomistic structure models in the PDB (possibly reconstructed from X-ray diffraction or NMR data, and 3D reconstructed cryo-electron microscopy (3D EM maps (albeit at coarser resolution of the same or homologous molecule or molecular assembly, deposited in the EMDB. To obtain the best possible structural model of the molecule at the best achievable resolution, and without any missing gaps, one typically aligns (match and fits the atomistic structure model with the 3D EM map. We discuss a new algorithm and generalized framework, named PF(2 fit (Polar Fast Fourier Fitting for the best possible structural alignment of atomistic structures with 3D EM. While PF(2 fit enables only a rigid, six dimensional (6D alignment method, it augments prior work on 6D X-ray structure and 3D EM alignment in multiple ways: Scoring. PF(2 fit includes a new scoring scheme that, in addition to rewarding overlaps between the volumes occupied by the atomistic structure and 3D EM map, rewards overlaps between the volumes complementary to them. We quantitatively demonstrate how this new complementary scoring scheme improves upon existing approaches. PF(2 fit also includes two scoring functions, the non-uniform exterior penalty and the skeleton-secondary structure score, and implements the scattering potential score as an alternative to traditional Gaussian blurring. Search. PF(2 fit utilizes a fast polar Fourier search scheme, whose main advantage is the ability to search over uniformly and adaptively sampled subsets of the space of rigid-body motions. PF(2 fit also implements a new reranking search and scoring methodology that considerably improves alignment metrics in results obtained from the initial search.

  16. The patellofemoral pain and osteoarthritis subscale of the KOOS (KOOS-PF): development and validation using the COSMIN checklist.

    Science.gov (United States)

    Crossley, Kay M; Macri, Erin M; Cowan, Sallie M; Collins, Natalie J; Roos, Ewa M

    2017-03-03

    Patellofemoral pain and osteoarthritis are prevalent and associated with substantial pain and functional impairments. Patient-reported outcome measures (PROMs) are recommended for research and clinical use, but no PROMs are specific for patellofemoral osteoarthritis, and existing PROMs for patellofemoral pain have methodological limitations. This study aimed to develop a new subscale of the Knee injury and Osteoarthritis Outcome Score for patellofemoral pain and osteoarthritis (KOOS-PF), and evaluate its measurement properties. Items were generated using input from 50 patients with patellofemoral pain and/or osteoarthritis and 14 health and medical clinicians. Item reduction was performed using data from patellofemoral cohorts (n=138). We used the COnsesus-based Standards for the selection of health Measurements INstruments guidelines to evaluate reliability, validity, responsiveness and interpretability of the final version of KOOS-PF and other KOOS subscales. From an initial 80 generated items, the final subscale included 11 items. KOOS-PF items loaded predominantly on one factor, pain during activities that load the patellofemoral joint. KOOS-PF had good internal consistency (Cronbach's α 0.86) and adequate test-retest reliability (intraclass correlation coefficient 0.86). Hypothesis testing supported convergent, divergent and known-groups validity. Responsiveness was confirmed, with KOOS-PF demonstrating a moderate correlation with Global Rating of Change scores (r 0.52) and large effect size (Cohen's d 0.89). Minimal detectable change was 2.3 (groups) and 16 (individuals), while minimal important change was 16.4. There were no floor or ceiling effects. The 11-item KOOS-PF, developed in consultation with patients and clinicians, demonstrated adequate measurement properties, and is recommended for clinical and research use in patients with patellofemoral pain and osteoarthritis. © Article author(s) (or their employer(s) unless otherwise stated in the text of

  17. Combination of acid-resistor and -scavenger improves the SEI stability and cycling ability of tin–nickel battery anodes in LiPF6-containing electrolyte

    International Nuclear Information System (INIS)

    Choo, Myeong-Ho; Nguyen, Cao Cuong; Hong, Sukhyun; Kwon, Yo Han; Woo, Sang-Wook; Kim, Je Young; Song, Seung-Wan

    2013-01-01

    Control of electrode–electrolyte interfacial reactivity and the formation of the solid electrolyte interphase (SEI) layer is a key technology for high performance rechargeable lithium batteries. Here we present the first report on a promising interfacial approach for Sn–Ni electrode that the use of acid-resisting and -scavenging fluorine-dopant on Sn combined with acid-scavenging trimethyl phosphite electrolyte additive to LiPF 6 -contiaing carbonate-based organic electrolyte improves the interfacial stability of Sn to acidic electrolyte species. As a result, a stable SEI layer consisting of a plenty of carbonate decomposition products forms and cycling ability significantly improves, in contrast to less efficient SEI formation and rapid performance fade for the electrodes without fluorine-dopant or trimethyl phosphite additive

  18. MHD equilibrium methods for ITER [International Thermonuclear Experimental Reactor] PF [poloidal field] coil design and systems analysis

    International Nuclear Information System (INIS)

    Strickler, D.J.; Galambos, J.D.; Peng, Y.K.M.

    1989-03-01

    Two versions of the Fusion Engineering Design Center (FEDC) free-boundary equilibrium code designed to computer the poloidal field (PF) coil current distribution of elongated, magnetically limited tokamak plasmas are demonstrated and applied to the systems analysis of the impact of plasma elongation on the design point of the International Thermonuclear Experimental Reactor (ITER). These notes were presented at the ITER Specialists' Meeting on the PF Coil System and Operational Scenario, held at the Max Planck Institute for Plasma Physics in Garching, Federal Republic of Germany, May 24--27, 1988. 8 refs., 6 figs., 4 tabs

  19. TRAC-PF1 MOD1 post test calculations of the OECD LOFT Experiment LP-SB-1

    International Nuclear Information System (INIS)

    Allen, E.J.

    1990-04-01

    Analysis of the small, hot leg break, OECD LOFT Experiment LP-SB-1. using the ''best-estimate'' computer code TRAC-PF1/MOD1 is presented. Descriptions of the LOFT facility and the LP-SB-1 experiment are given and development of the TRAC-PF1/MOD1 input model is detailed. The calculations performed in achieving the steady state conditions, from which the experiment was initiated, and the specification of experimental boundary conditions are outlined. 24 refs., 66 figs., 12 tabs

  20. Tumor P-Glycoprotein Correlates with Efficacy of PF-3758309 in in vitro and in vivo Models of Colorectal Cancer

    OpenAIRE

    Bradshaw-Pierce, Erica Lynn; Pitts, Todd M.; Tan, Aik-Choon; McPhillips, Kelly; West, Mark; Gustafson, Daniel L.; Halsey, Charles; Nguyen, Leslie; Lee, Nathan V.; Kan, Julie L. C.; Murray, Brion William; Eckhardt, S. Gail

    2013-01-01

    P-glycoprotein (P-gp), a member of the ATP-binding cassette transporter family, is overexpressed in a number of different cancers and some studies show that P-gp overexpression can be correlated to poor prognosis or therapeutic resistance. Here we sought to elucidate if PF-3758309 (PF-309), a novel p-21 activated kinase inhibitor, efficacy was influenced by tumor P-gp. Based on in vitro proliferation data, a panel of colorectal cancer cell lines were ranked as sensitive or resistant and ABCB1...

  1. Tumor p-glycoprotein correlates with efficacy of PF-3758309 in in vitro and in vivo models of colorectal cancer.

    OpenAIRE

    Erica Lynn Bradshaw-Pierce; Erica Lynn Bradshaw-Pierce; Todd M Pitts; Aik-Choon eTan; Kelly eMcPhillips; Mark eWest; Daniel L Gustafson; Charles eHalsey; Leslie eNguyen; Nathan V Lee; Julie LC Kan; Brion William Murray; S. Gail eEckhardt

    2013-01-01

    P-glycoprotein (P-gp), a member of the ATP-binding cassette transporter family, is overexpressed in a number of different cancers and some studies show that P-gp overexpression can be correlated to poor prognosis or therapeutic resistance. Here we sought to elucidate if PF-3758309 (PF-309), a novel p-21 activated kinase inhibitor, efficacy was influenced by tumor P-gp. Based on in vitro proliferation data, a panel of colorectal cancer cell lines were ranked as sensitive or resistant and ABCB...

  2. Valence shell photoionization energies and cross-sections of NF sub 3 and PF sub 3

    CERN Document Server

    Jürgensen, A

    2003-01-01

    Relative outer valence shell ionization potentials and cross-sections were determined for the isostructural, Group 15, trifluorides NF sub 3 and PF sub 3 in the gas phase using synchrotron radiation. Excitation photon energies ranged from 70 to 160 eV. The experimental spectra were assigned and cross-sections analyzed with the aid of both MS-X alpha and ab initio calculations. Spectral differences in peak energies and relative intensities are related to structural and electronic differences between these two fluoride molecules. Valence shell ionization potentials were compared to calculated values obtained by several different methods. The partial photoionization cross-sections for each orbital were obtained as a function of excitation energy and compared to theoretical results obtained with the X alpha method.

  3. TRAC-PF1 code verification with data from the OTIS test facility

    International Nuclear Information System (INIS)

    Childerson, M.T.; Fujita, R.K.

    1985-01-01

    A computer code (TRAC-PF1/MOD1) developed for predicting transient thermal and hydraulic integral nuclear steam supply system (NSSS) response was benchmarked. Post-small break loss-of-coolant accident (LOCA) data from a scaled, experimental facility, designated the One-Through Integral System (OTIS), were obtained for the Babcock and Wilcox NSSS and compared to TRAC predictions. The OTIS tests provided a challenging small break LOCA data set for TRAC verification. The major phases of a small break LOCA observed in the OTIS tests included pressurizer draining and loop saturation, intermittent reactor coolant system circulation, boiler-condenser mode, and the initial stages of refill. The TRAC code was successful in predicting OTIS loop conditions (system pressures and temperatures) after modification of the steam generator model. In particular, the code predicted both pool and auxiliary-feedwater initiated boiler-condenser mode heat transfer

  4. Discovery of PF-04620110, a Potent, Selective, and Orally Bioavailable Inhibitor of DGAT-1.

    Science.gov (United States)

    Dow, Robert L; Li, Jian-Cheng; Pence, Michael P; Gibbs, E Michael; LaPerle, Jennifer L; Litchfield, John; Piotrowski, David W; Munchhof, Michael J; Manion, Tara B; Zavadoski, William J; Walker, Gregory S; McPherson, R Kirk; Tapley, Susan; Sugarman, Eliot; Guzman-Perez, Angel; DaSilva-Jardine, Paul

    2011-05-12

    Acyl-CoA:diacylglycerol acyltransferase-1 (DGAT-1) catalyzes the final committed step in the biosynthesis of triglycerides. DGAT-1 knockout mice have been shown to be resistant to diet-induced obesity and have increased insulin sensitivity. Thus, inhibition of DGAT-1 may represent an attractive target for the treatment of obesity or type II diabetes. Herein, we report the discovery and characterization of a potent and selective DGAT-1 inhibitor PF-04620110 (3). Compound 3 inhibits DGAT-1 with an IC50 of 19 nM and shows high selectivity versus a broad panel of off-target pharmacologic end points. In vivo DGAT-1 inhibition has been demonstrated through reduction of plasma triglyceride levels in rodents at doses of ≥0.1 mg/kg following a lipid challenge. On the basis of this pharmacologic and pharmacokinetic profile, compound 3 has been advanced to human clinical studies.

  5. Independent Review of Siesmic Performance Assessments for the Plutonium Facility PF-4

    Energy Technology Data Exchange (ETDEWEB)

    Whittaker, Andrew [State Univ. of New York (SUNY), Buffalo, NY (United States); Goen, Lawrence Kenneth [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Kennedy, Robert [RPK Structural Mechanics, San Diego, CA (United States); McDonald, Brian [Exponent, Los Angeles, CA (United States); Morgan, Troy [Exponent, Los Angeles, CA (United States); Wyllie, Loring [Degenkolb Engineers, San Francisco, CA (United States)

    2015-11-25

    The Plutonium Facility, designated PF-4, is located in Technical Area 55 at the Los Alamos National Laboratory (LANL). The facility is a one-story rectangular structure above a complete basement; the building was constructed of cast-in-place reinforced concrete, with small interior frames of structural steel. The plan dimensions of the building are 265’×284’. The overall height of the building varies between 39’-0” at the north and south ends, and 40’-6” at the center ridge. The programmatic work performed in the building is vital to our national security and its functions and storage purposes are not replicated elsewhere in the United States Department of Energy (DOE).

  6. Status of PF-ring commissioning after the reconstruction for the straight-sections upgrade

    International Nuclear Information System (INIS)

    Tanimoto, Yasunori; Miyauchi, Hiroshi; Honda, Tohru; Kobayashi, Yukinori

    2006-01-01

    The upgrade project of the straight sections at the PF-ring enables the reinforcement of insertion devices and their beamlines. As a main part of this project, a large-scale reconstruction of the storage ring was carried out from March to September in 2005. The lattice configuration was modified to create short straight sections of 1.4 m and to extend the existing straight sections. Optical functions of the short sections were optimized for short-gap mini-pole undulators as X-ray sources. The first mini-pole undulator SGU no.17 has been operated for a protein crystallography. A recommissioning of the ring was conducted for about one month, and user experiments were restarted on 18th October 2005 according to schedule. The recovery of the beam lifetime due to the process of the vacuum scrubbing has been favorably progressed. (author)

  7. Met tyrosine kinase inhibitor, PF-2341066, suppresses growth and invasion of nasopharyngeal carcinoma

    Directory of Open Access Journals (Sweden)

    Zhao Y

    2015-08-01

    Full Text Available Yuanyuan Zhao,1,* Jing Zhang,2,* Ying Tian,1,* Cong Xue,1 Zhihuang Hu,1 Li Zhang1,3 1Department of Medical Oncology, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, and Collaborative Innovation Center for Cancer Medicine, 2Department of Medical Oncology, the First Affiliated Hospital of Guang Zhou Traditional Chinese Medicine University, 3National Anti-Cancer Drug Research Centre, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, and Collaborative Innovation Center for Cancer Medicine, Guangzhou, People’s Republic of China*These authors contributed equally to this work Purpose: We explored the effect of hepatocyte growth factor (HGF/Met signaling pathway on nasopharyngeal carcinoma (NPC cells in vitro and in vivo, and investigated the ability of Met tyrosine kinase inhibitor (TKI to block HGF-induced biological signaling.Experimental design: Met TKI inhibitor PF-2341066 alone, or in combination with cisplatin, was investigated for its ability to block HGF-induced signaling and biological effects in vitro and in vivo. HGF/Met expression and activation of signaling in NPC cells were detected by using Western blot and immunohistochemistry. Biological evaluation, including wound healing, cell proliferation, and invasion of NPC cells, was also examined, and the correlation between HGF/Met expression of primary and metastatic tumor in NPC patients and clinical prognosis were also analyzed.Results: Met TKI inhibitor, PF-2341066, inhibited growth of NPC cells in vivo with half maximal inhibitory concentration of 0.79±0.21 µmol/L, and suppressed invasion and migration of NPC cells; also, the inhibition of PF-2341066 was synergized with cisplatin treatment. Compared with the control group, Met TKI inhibited metastasis of transplanted NPC in nude mice (the number of live metastases [mean ± SD]: 5.8±2.2 versus 11.8±2.2, P=0.03; the number of lung metastases: 2.3±1.5 versus

  8. The patellofemoral pain and osteoarthritis subscale of the KOOS (KOOS-PF)

    DEFF Research Database (Denmark)

    Crossley, Kay M; Macri, Erin M; Cowan, Sallie M

    2018-01-01

    with patellofemoral pain and/or osteoarthritis and 14 health and medical clinicians. Item reduction was performed using data from patellofemoral cohorts (n=138). We used the COnsesus-based Standards for the selection of health Measurements INstruments guidelines to evaluate reliability, validity, responsiveness......BACKGROUND: Patellofemoral pain and osteoarthritis are prevalent and associated with substantial pain and functional impairments. Patient-reported outcome measures (PROMs) are recommended for research and clinical use, but no PROMs are specific for patellofemoral osteoarthritis, and existing PROMs...... for patellofemoral pain have methodological limitations. This study aimed to develop a new subscale of the Knee injury and Osteoarthritis Outcome Score for patellofemoral pain and osteoarthritis (KOOS-PF), and evaluate its measurement properties. METHODS: Items were generated using input from 50 patients...

  9. Statistical fluctuations of electromagnetic transition intensities in pf-shell nuclei

    International Nuclear Information System (INIS)

    Hamoudi, A.; Nazmitdinov, R.G.; Shakhaliev, E.; Alhassid, Y.

    2000-01-01

    We study the fluctuation properties of ΔT = 0 electromagnetic transition intensities in A ∼ 60 nuclei within the framework of the interacting shell model, using a realistic effective interaction for pf-shell nuclei with a 56 Ni core. It is found that the B(E2) and the ΔJ ≠ 0 distributions are well described by the Gaussian orthogonal ensemble of random matrices (Porter-Thomas distribution) independently of the isobaric quantum number T Z . However, the statistics of the B(M1) transitions with Δ = 0 are sensitive to T Z : T Z = 1 nuclei exhibit a Porter-Thomas distribution, while a significant deviation from the GOE statistics is observed for self-conjugate nuclei (T Z = 0). Similar results are found for A = 22 sd-shell nuclei

  10. TRAC-PF1: an advanced best-estimate computer program for pressurized water reactor analysis

    International Nuclear Information System (INIS)

    Liles, D.R.; Mahaffy, J.H.

    1984-02-01

    The Transient Reactor Analysis Code (TRAC) is being developed at the Los Alamos National Laboratory to provide advanced best-estimate predictions of postulated accidents in light water reactors. The TRAC-PF1 program provides this capability for pressurized water reactors and for many thermal-hydraulic experimental facilities. The code features either a one-dimensional or a three-dimensional treatment of the pressure vessel and its associated internals; a two-phase, two-fluid nonequilibrium hydrodynamics model with a noncondensable gas field; flow-regime-dependent constitutive equation treatment; optional reflood tracking capability for both bottom flood and falling-film quench fronts; and consistent treatment of entire accident sequences including the generation of consistent initial conditions. This report describes the thermal-hydraulic models and the numerical solution methods used in the code. Detailed programming and user information also are provided

  11. An overview of the TA-55, Building PF-4 ventilation system

    International Nuclear Information System (INIS)

    1994-01-01

    An overview of the TA-55, Building PF-4 ventilation system is provided in the following sections. Included are descriptions of the zone configurations, equipment-performance criteria, ventilation support systems, and the ventilation-system evaluation criteria. Section 4.2.1.1 provides a brief discussion of the ventilation system function. Section 4.2.1.2 provides details on the overall system configuration. Details of system interfaces and support systems are provided in Section 4.2.1.3. Section 4.2.1.4 describes instrumentation and control needed to operate the ventilation system. Finally, Sections 4.2.1.5 and 4.2.1.6 describe system surveillance/maintenance and Technical Safety Requirements (TSR) Limitations, respectively. Note that the numerical parameters included in this description are considered nominal; set points and other specifications actually fall within operational bands

  12. PF-AR NW14, a new time-resolved diffraction/scattering beamline

    International Nuclear Information System (INIS)

    Nozawa, Shunsuke; Adachi, Shin-ichi; Tazaki, Ryoko; Takahashi, Jun-ichi; Itatani, Jiro; Daimon, Masahiro; Mori, Takeharu; Sawa, Hiroshi; Kawata, Hiroshi; Koshihara, Shin-ya

    2005-01-01

    NW14 is a new insertion device beamline at the Photon Factory Advanced Ring (PF-AR), which is a unique ring with full-time single-bunched operation, aiming for timeresolved x-ray diffraction/scattering and XAFS experiments. The primary scientific goal of this beamline is to observe the ultrafast dynamics of condensed matter systems such as organic and inorganic crystals, biological systems and liquids triggered by optical pulses. With the large photon fluxes derived from the undulator, it should become possible to take a snapshoot an atomic-scale image of the electron density distribution. By combining a series of images it is possible to produce a movie of the photo-induced dynamics with 50-ps resolution. The construction of the beamline is being funded by the ERATO Koshihara Non-equilibrium Dynamics Project of the Japan Science and Technology Agency (JST), and the beamline will be operational from autumn 2005

  13. Power law field dependence of the 2D magnetoresistance in (TMTSF)2PF6

    International Nuclear Information System (INIS)

    Kriza, G.; Szeghy, G.; Kezsmarki, I.; Mihaly, G.

    1999-01-01

    The magnetoresistance of the quasi-one-dimensional organic conductor (TMTSF) 2 PF 6 is studied for currents flowing parallel to the best conducting a and second best conducting b directions in magnetic fields perpendicular to the a-b plane under a hydrostatic pressure of 0.8 GPa. As a function of the magnetic field, the magnetoresistance follows a power law ΔR/R = (B/B 0 ) 3/2 both in the a and b directions. The a-b plane conductivity anisotropy is field independent. The scaling field B 0 , characterizing the strength of the magnetoresistance, follows an exponential temperature dependence B 0 ∝exp(T/T 0 ) with a field-independent characteristic temperature T 0 = 10 K. (orig.)

  14. Galvanomagnetic properties in the spin-density-wave phase of (TMTSF)2PF6

    International Nuclear Information System (INIS)

    Korin-Hamzic, B.; Bechgaard, K.

    1999-01-01

    We have measured the magnetoresistance and the Hall effect in the spin-density-wave (SDW) state of (TMTSF) 2 PF 6 down to 2 K and in magnetic fields up to 9 T in order to gain an additional insight into the nature of the possible subphases in the SDW state. We have found that the temperature dependent magnetoresistance anisotropy changes below 4 K; this change being maximal for the current in the lowest conductivity direction. The Hall resistivity has showed different magnetic field dependencies for T>4 K and T<4 K. The resistivity and the Hall resistivity were also investigated as the function of the electric field. The backflow coefficient α does not change below 4 K. (orig.)

  15. Anisotropic p-f mixing mechanism explaining anomalous magnetic properties in Ce monopnictides

    International Nuclear Information System (INIS)

    Takahashi, H.; Kasuya, T.

    1985-01-01

    An anomalously small crystal-field splitting in the paramagnetic region in CeSb and CeBi compared with those in CeP and CeAs is explained by the mixing mechanism between the occupied 4f states and the valence band holes. In the paramagnetic regions, the above p-f mixing gives the effective crystal-field splitting, which is estimated to nearly cancel the normal splittings extrapolated from CeP and CeAs in good agreement with experiment. The formula for the second-order transition temperature, at which the population ratio of the 4f GAMMA 8 states begins to be unbalanced, is also derived. In CeBi the second-order transition may occur, while in CeSb the first-order transition should occur in agreement with experiments. (author)

  16. PF-AR NW14, a new time-resolved diffraction/scattering beamline

    Science.gov (United States)

    Nozawa, Shunsuke; Adachi, Shin-ichi; Tazaki, Ryoko; Takahashi, Jun-ichi; Itatani, Jiro; Daimon, Masahiro; Mori, Takeharu; Sawa, Hiroshi; Kawata, Hiroshi; Koshihara, Shin-ya

    2005-01-01

    NW14 is a new insertion device beamline at the Photon Factory Advanced Ring (PF-AR), which is a unique ring with full-time single-bunched operation, aiming for timeresolved x-ray diffraction/scattering and XAFS experiments. The primary scientific goal of this beamline is to observe the ultrafast dynamics of condensed matter systems such as organic and inorganic crystals, biological systems and liquids triggered by optical pulses. With the large photon fluxes derived from the undulator, it should become possible to take a snapshoot an atomic-scale image of the electron density distribution. By combining a series of images it is possible to produce a movie of the photo-induced dynamics with 50-ps resolution. The construction of the beamline is being funded by the ERATO Koshihara Non-equilibrium Dynamics Project of the Japan Science and Technology Agency (JST), and the beamline will be operational from autumn 2005.

  17. Study of the Plasma Evolution in the PF-1000 Device by Means of Optical Diagnostics

    International Nuclear Information System (INIS)

    Kasperczuk, A.; Kumar, R.; Miklaszewski, R.; Paduch, M.; Pisarczyk, T.; Scholz, M.; Tomaszewski, K.

    2002-01-01

    Investigation of a plasma evolution was carried out in a PF-1000 device with the following parameters: diameter of the inner electrode -24.4 cm, diameter of the outer one -36.8 cm, charging voltage in the range of 30-40 kV and deuterium pressure in the range of 1-5 1 To study the evolution of the plasma, an optical frame camera and YAGlaser shadowgraphy, both with exposure times of about 1 ns, were employed. Among the cases analyzed of plasma focus discharges, two types of plasma sheath disturbance can be distinguished: type I - classical MHD m = 0 instability (a wave with four maxima in the main), type 11 - singular great scale disturbance. On the basis of the plasma images, the dynamics of the plasma sheath, characteristic periods of the plasma evolution and plasma dimensions were determined. In order to reconstruct the spatial distribution of the electron density a special method was prepared

  18. An overview of the TA-55, Building PF-4 ventilation system

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-02-22

    An overview of the TA-55, Building PF-4 ventilation system is provided in the following sections. Included are descriptions of the zone configurations, equipment-performance criteria, ventilation support systems, and the ventilation-system evaluation criteria. Section 4.2.1.1 provides a brief discussion of the ventilation system function. Section 4.2.1.2 provides details on the overall system configuration. Details of system interfaces and support systems are provided in Section 4.2.1.3. Section 4.2.1.4 describes instrumentation and control needed to operate the ventilation system. Finally, Sections 4.2.1.5 and 4.2.1.6 describe system surveillance/maintenance and Technical Safety Requirements (TSR) Limitations, respectively. Note that the numerical parameters included in this description are considered nominal; set points and other specifications actually fall within operational bands.

  19. Statistical fluctuations of electromagnetic transition intensities and electromagnetic moments in pf-shell nuclei

    International Nuclear Information System (INIS)

    Hamoudi, A.; Shahaliev, E.; Nazmitdinov, R. G.; Alhassid, Y.

    2002-01-01

    We study the fluctuation properties of ΔT=0 electromagnetic transition intensities and electromagnetic moments in A∼60 nuclei within the framework of the interacting shell model, using a realistic effective interaction for pf-shell nuclei with a 56 Ni core. The distributions of the transition intensities and of the electromagnetic moments are well described by the Gaussian orthogonal ensemble of random matrices. In particular, the transition intensity distributions follow a Porter-Thomas distribution. When diagonal matrix elements (i.e., moments) are included in the analysis of transition intensities, the distributions remain Porter-Thomas except for the isoscalar M1. This deviation is explained in terms of the structure of the isoscalar M1 operator

  20. [Study on method of tracking the active cells in image sequences based on EKF-PF].

    Science.gov (United States)

    Tang, Chunming; Liu, Ying

    2013-02-01

    In cell image sequences, due to the nonlinear and nonGaussian motion characteristics of active cells, the accurate prediction and tracking is still an unsolved problem. We applied extended Kalman particle filter (EKF-PF) here in our study, attempting to solve the problem. Firstly we confirmed the existence and positions of the active cells. Then we established a motion model and improved it via adding motion angle estimation. Next we predicted motion parameters, such as displacement, velocity, accelerated velocity and motion angle, in region centers of the cells being tracked. Finally we obtained the motion traces of active cells. There were fourteen active cells in three image sequences which have been tracked. The errors were less than 2.5 pixels when the prediction values were compared with actual values. It showed that the presented algorithm may basically reach the solution of accurate predition and tracking of the active cells.

  1. Multiscale Simulations of Lamellar PS–PEO Block Copolymers Doped with LiPF6 Ions

    KAUST Repository

    Sethuraman, Vaidyanathan

    2017-06-02

    We report the results of atomistic simulations of the structural equilibrium properties of PS–PEO block copolymer (BCP) melt in the ordered lamellar phase doped with LiPF6 salt. A hybrid simulation strategy, consisting of steps of coarse-graining and inverse coarse-graining, was employed to equilibrate the melt at an atomistic resolution in the ordered phase. We characterize the structural distributions between different atoms/ions and compare the features arising in BCPs against the corresponding behavior in PEO homopolymers for different salt concentrations. In addition, the local structural distributions are characterized in the lamellar phase as a function of distance from the interface. The cation–anion radial distribution functions (RDF) display stronger coordination in the block copolymer melts at high salt concentrations, whereas the trends are reversed for low salt concentrations. Radial distribution functions isolated in the PEO and PS domains demonstrate that the stronger coordination seen in BCPs arises from the influence of both the higher fraction of ions segregated in the PS phase and the influence of interactions in the PS domain. Such a behavior also manifests in the cation–anion clusters, which show a larger fraction of free ions in the BCP. While the average number of free anions (cations) decreases with increasing salt concentration, higher order aggregates of LiPF6 increase with increasing salt concentration. Further, the cation–anion RDFs display spatial heterogeneity, with a stronger cation–anion binding in the interfacial region compared to bulk of the PEO domain.

  2. Measurements of nitrous oxide emissions from P.F. fired power stations

    Science.gov (United States)

    Sloan, S. A.; Laird, C. K.

    Nitrous oxide (N 2O) was measured in the flue gas from four wall-fired and three corner-fired 500 MW boilers, fitted with either conventional or low-NO x burners, at four C.E.G.B. power stations. Measurements were made both by on-line non-dispersive infra red (NDIR) monitors and by extractive sampling for later laboratory analysis by electron capture gas chromatography (ECD GC). It was found that the on-line and laboratory methods were in good agreement for samples analyzed within 1-3 h of sampling, but that the nitrous oxide concentration in the stored samples had a tendency to increase with time, which was dependent on the concentration of water in the sample. Experiments with synthetic mixtures showed that the increase in nitrous oxide concentration was consistent with the overall reaction2NO+SO 2→N 2O+SO 3 in which nitric oxide is reduced by sulphur dioxide. The highest average N 2O concentration measured by the on-line analyzer was 16 vpm, and for most of the boilers monitored the concentration was less than 10 vpm. There was no statistically significant difference between the measured N 2O emissions from normal boilers and those fitted with low-NO x burners. It is suggested that these values are close to the true levels of N 2O emissions from p.f. fired boilers and that recent reports of high levels (up to 200 vpm) are likely to be an artefact resulting from the observed generation of N 2O in stored samples. A consequence of these measurements of N 2O is that current estimates of the contribution of emissions from p.f. fired boilers to the global N 2O budget are likely to be too high.

  3. Heart failure - medicines

    Science.gov (United States)

    CHF - medicines; Congestive heart failure - medicines; Cardiomyopathy - medicines; HF - medicines ... You will need to take most of your heart failure medicines every day. Some medicines are taken ...

  4. Lessons learned from failure analysis

    International Nuclear Information System (INIS)

    Le May, I.

    2006-01-01

    Failure analysis can be a very useful tool to designers and operators of plant and equipment. It is not simply something that is done for lawyers and insurance companies, but is a tool from which lessons can be learned and by means of which the 'breed' can be improved. In this presentation, several failure investigations that have contributed to understanding will be presented. Specifically, the following cases will be discussed: 1) A fire at a refinery that occurred in a desulphurization unit. 2) The failure of a pipeline before it was even put into operation. 3) Failures in locomotive axles that took place during winter operation. The refinery fire was initially blamed on defective Type 321 seamless stainless steel tubing, but there were conflicting views between 'experts' involved as to the mechanism of failure and the writer was called upon to make an in-depth study. This showed that there were a variety of failure mechanism involved, including high temperature fracture, environmentally-induced cracking and possible manufacturing defects. The unraveling of the failure sequence is described and illustrated. The failure of an oil transmission was discovered when the line was pressure tested some months after it had been installed and before it was put into service. Repairs were made and failure occurred in another place upon the next pressure test being conducted. After several more repairs had been made the line was abandoned and a lawsuit was commenced on the basis that the steel was defective. An investigation disclosed that the material was sensitive to embrittlement and the causes of this were determined. As a result, changes were made in the microstructural control of the product to avoid similar problems in future. A series of axle failures occurred in diesel electric locomotives during winter. An investigation was made to determine the nature of the failures which were not by classical fatigue, nor did they correspond to published illustrations of Cu

  5. The Exported Chaperone PfHsp70x Is Dispensable for the Plasmodium falciparum Intraerythrocytic Life Cycle.

    Science.gov (United States)

    Cobb, David W; Florentin, Anat; Fierro, Manuel A; Krakowiak, Michelle; Moore, Julie M; Muralidharan, Vasant

    2017-01-01

    Export of parasite proteins into the host erythrocyte is essential for survival of Plasmodium falciparum during its asexual life cycle. While several studies described key factors within the parasite that are involved in protein export, the mechanisms employed to traffic exported proteins within the host cell are currently unknown. Members of the Hsp70 family of chaperones, together with their Hsp40 cochaperones, facilitate protein trafficking in other organisms, and are thus likely used by P. falciparum in the trafficking of its exported proteins. A large group of Hsp40 proteins is encoded by the parasite and exported to the host cell, but only one Hsp70, P. falciparum Hsp70x (PfHsp70x), is exported with them. PfHsp70x is absent in most Plasmodium species and is found only in P. falciparum and closely related species that infect apes. Herein, we have utilized clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 genome editing in P. falciparum to investigate the essentiality of PfHsp70x. We show that parasitic growth was unaffected by knockdown of PfHsp70x using both the dihydrofolate reductase (DHFR)-based destabilization domain and the glmS ribozyme system. Similarly, a complete gene knockout of PfHsp70x did not affect the ability of P. falciparum to proceed through its intraerythrocytic life cycle. The effect of PfHsp70x knockdown/knockout on the export of proteins to the host red blood cell (RBC), including the critical virulence factor P. falciparum erythrocyte membrane protein 1 (PfEMP1), was tested, and we found that this process was unaffected. These data show that although PfHsp70x is the sole exported Hsp70, it is not essential for the asexual development of P. falciparum . IMPORTANCE Half of the world's population lives at risk for malaria. The intraerythrocytic life cycle of Plasmodium spp. is responsible for clinical manifestations of malaria; therefore, knowledge of the parasite's ability to survive within the erythrocyte is

  6. Diagnosing severe falciparum malaria in parasitaemic African children: a prospective evaluation of plasma PfHRP2 measurement.

    Directory of Open Access Journals (Sweden)

    Ilse C E Hendriksen

    Full Text Available In African children, distinguishing severe falciparum malaria from other severe febrile illnesses with coincidental Plasmodium falciparum parasitaemia is a major challenge. P. falciparum histidine-rich protein 2 (PfHRP2 is released by mature sequestered parasites and can be used to estimate the total parasite burden. We investigated the prognostic significance of plasma PfHRP2 and used it to estimate the malaria-attributable fraction in African children diagnosed with severe malaria.Admission plasma PfHRP2 was measured prospectively in African children (from Mozambique, The Gambia, Kenya, Tanzania, Uganda, Rwanda, and the Democratic Republic of the Congo aged 1 month to 15 years with severe febrile illness and a positive P. falciparum lactate dehydrogenase (pLDH-based rapid test in a clinical trial comparing parenteral artesunate versus quinine (the AQUAMAT trial, ISRCTN 50258054. In 3,826 severely ill children, Plasmadium falciparum PfHRP2 was higher in patients with coma (p = 0.0209, acidosis (p<0.0001, and severe anaemia (p<0.0001. Admission geometric mean (95%CI plasma PfHRP2 was 1,611 (1,350-1,922 ng/mL in fatal cases (n = 381 versus 1,046 (991-1,104 ng/mL in survivors (n = 3,445, p<0.0001, without differences in parasitaemia as assessed by microscopy. There was a U-shaped association between log(10 plasma PfHRP2 and risk of death. Mortality increased 20% per log(10 increase in PfHRP2 above 174 ng/mL (adjusted odds ratio [AOR] 1.21, 95%CI 1.05-1.39, p = 0.009. A mechanistic model assuming a PfHRP2-independent risk of death in non-malaria illness closely fitted the observed data and showed malaria-attributable mortality less than 50% with plasma PfHRP2≤174 ng/mL. The odds ratio (OR for death in artesunate versus quinine-treated patients was 0.61 (95%CI 0.44-0.83, p = 0.0018 in the highest PfHRP2 tertile, whereas there was no difference in the lowest tertile (OR 1.05; 95%CI 0.69-1.61; p = 0.82. A limitation of the study is that some

  7. Hypogonadism and renal failure: An update.

    Science.gov (United States)

    Thirumavalavan, Nannan; Wilken, Nathan A; Ramasamy, Ranjith

    2015-01-01

    The prevalence of both hypogonadism and renal failure is increasing. Hypogonadism in men with renal failure carries with it significant morbidity, including anemia and premature cardiovascular disease. It remains unclear whether testosterone therapy can affect the morbidity and mortality associated with renal failure. As such, in this review, we sought to evaluate the current literature addressing hypogonadism and testosterone replacement, specifically in men with renal failure. The articles chosen for this review were selected by performing a broad search using Pubmed, Embase and Scopus including the terms hypogonadism and renal failure from 1990 to the present. This review is based on both primary sources as well as review articles. Hypogonadism in renal failure has a multifactorial etiology, including co-morbid conditions such as diabetes, hypertension, old age and obesity. Renal failure can lead to decreased luteinizing hormone production and decreased prolactin clearance that could impair testosterone production. Given the increasing prevalence of hypogonadism and the potential morbidity associated with hypogonadism in men with renal failure, careful evaluation of serum testosterone would be valuable. Testosterone replacement therapy should be considered in men with symptomatic hypogonadism and renal failure, and may ameliorate some of the morbidity associated with renal failure. Patients with all stages of renal disease are at an increased risk of hypogonadism that could be associated with significant morbidity. Testosterone replacement therapy may reduce some of the morbidity of renal failure, although it carries risk.

  8. 27 CFR 25.177 - Evasion of or failure to pay tax; failure to file a tax return.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Evasion of or failure to... Pay Tax § 25.177 Evasion of or failure to pay tax; failure to file a tax return. Sections 5671, 5673, 5684, 6651, and 6656 of Title 26 United States Code provide penalties for evasion or failure to pay tax...

  9. Learning from Failures in Operations

    DEFF Research Database (Denmark)

    Koch, Christian

    2005-01-01

    parallelism and fragmentation due to their predominantly quantitative complexity. It is suggested that a number of critical junctions occur in these processes and that obtaining quality and avoiding failures quite often requires a series of competent and situational operation management actions. An empirical...... product. The analysis showed that, among others, design review, project review, commencing and finalizing operations turned out to be critical junctions in the control of failures. Practical implications and improvements are discussed. The focus on critical junctions, points to supply chain management......This paper discusses failures and quality in operations in design and production of buildings. The importance of human action, interpretations and interactions in tackling complexity, fragmentation and disturbances in the building industry is underlined. Building processes encompass requisite...

  10. Multidimensional protein fractionation using ProteomeLab PF 2D™ for profiling amyotrophic lateral sclerosis immunity: A preliminary report

    Directory of Open Access Journals (Sweden)

    Mosley R Lee

    2008-09-01

    Full Text Available Abstract Background The ProteomeLab™ PF 2D platform is a relatively new approach to global protein profiling. Herein, it was used for investigation of plasma proteome changes in amyotrophic lateral sclerosis (ALS patients before and during immunization with glatiramer acetate (GA in a clinical trial. Results The experimental design included immunoaffinity depletion of 12 most abundant proteins from plasma samples with the ProteomeLab™ IgY-12 LC10 column kit as first dimension separation, also referred to as immuno-partitioning. Second and third dimension separations of the enriched proteome were performed on the PF 2D platform utilizing 2D isoelectric focusing and RP-HPLC with the resulting fractions collected for analysis. 1D gel electrophoresis was added as a fourth dimension when sufficient protein was available. Protein identification from collected fractions was performed using nano-LC-MS/MS approach. Analysis of differences in the resulting two-dimensional maps of fractions obtained from the PF 2D and the ability to identify proteins from these fractions allowed sensitivity threshold measurements. Masked proteins in the PF 2D fractions are discussed. Conclusion We offer some insight into the strengths and limitations of this emerging proteomic platform.

  11. Input data preparation and simulation of the second standard problem of IAEA using the Trac/PF1 code

    International Nuclear Information System (INIS)

    Madeira, A.A.; Pontedeiro, A.C.; Silva Galetti, M.R. da; Borges, R.C.

    1989-10-01

    The second Standard Problem sponsored by IAEA consists in the simulation of a small LOCA located in the downcomer of a PMK-NVH integral test facility, which models WWER/440 type reactor. This report presents input data preparation and comparison between TRAC-PF1 results and experimental measurements. (author) [pt

  12. Antigenic variation and the genetics and epigenetics of the PfEMP1 erythrocyte surface antigens in Plasmodium falciparum malaria

    DEFF Research Database (Denmark)

    Arnot, David E; Jensen, Anja T R

    2011-01-01

    . Sterile immunity is not achieved and chronic parasitization of apparently healthy adults is the norm. In this article, we analyse the best understood malaria "antigenic variation" system, that based on Plasmodium falciparum's PfEMP1-type cytoadhesion antigens, and critically review recent literature...

  13. Phloroglucinol mediates crosstalk between the pyoluteorin and 2,4-diacetylphloroglucinol biosynthetic pathways in Pseudomonas fluorescens Pf-5

    Science.gov (United States)

    The antibiotics pyoluteorin and 2,4-diacetylphloroglucinol (DAPG) are involved in the biological control of certain soil-borne diseases by some strains of Pseudomonas fluorescens, including P. fluorescens Pf-5. These secondary metabolites also act as signaling molecules with each compound reported ...

  14. PfClpC Is an Essential Clp Chaperone Required for Plastid Integrity and Clp Protease Stability in Plasmodium falciparum

    Directory of Open Access Journals (Sweden)

    Anat Florentin

    2017-11-01

    Full Text Available Summary: The deadly malaria parasite Plasmodium falciparum contains a nonphotosynthetic plastid, known as the apicoplast, that functions to produce essential metabolites, and drugs that target the apicoplast are clinically effective. Several prokaryotic caseinolytic protease (Clp genes have been identified in the Plasmodium genome. Using phylogenetic analysis, we focused on the Clp members that may form a regulated proteolytic complex in the apicoplast. We genetically targeted members of this complex and generated conditional mutants of the apicoplast-localized PfClpC chaperone and PfClpP protease. Conditional inhibition of the PfClpC chaperone resulted in growth arrest and apicoplast loss and was rescued by addition of the essential apicoplast-derived metabolite IPP. Using a double-conditional mutant parasite line, we discovered that the chaperone activity is required to stabilize the mature protease, revealing functional interactions. These data demonstrate the essential function of PfClpC in maintaining apicoplast integrity and its role in regulating the proteolytic activity of the Clp complex. : Plasmodium falciparum contains a unique organelle, the apicoplast. Using genetic and phenotypic assays, Florentin et al. characterize the apicoplast Clp chaperone and protease. They find that the chaperone is essential for protease stability and that together they function to maintain organelle integrity and segregation into daughter cells. Keywords: malaria, Plasmodium, apicoplast, IPP, Clp, chaperone, caseinolytic protease

  15. Disruption of transporters affiliated with enantio-pyochelin biosynthesis gene cluster of Pseudomonas protegens Pf-5 has pleiotropic effects

    Science.gov (United States)

    Pseudomonas protegens Pf-5 (formerly Pseudomonas fluorescens) is a biocontrol bacterium that produces the siderophore enantio-pyochelin under conditions of iron starvation in a process that is often accompanied by the secretion of its biosynthesis intermediates, salicylic acid and dihydroaeruginoic ...

  16. Diagnostic systems for the nuclear fusion and plasma research in the PF-24 plasma focus laboratory at the IFJ PAN

    Directory of Open Access Journals (Sweden)

    Marciniak Łukasz

    2016-12-01

    Full Text Available This paper presents a set of diagnostics dedicated to PF-24 - new medium size - plasma focus (PF device built and operated at the Institute of Nuclear Physics Polish Academy of Sciences (IFJ PAN. The PF-24 can operate at energy level up to 93 kJ and charging voltage up to 40 kV. Each condenser is connected with a specially designed spark gap with a very small jitter, which ensures a high effi ciency and a low current rise time. The working parameters of PF-24 generator make it a suitable tool for testing new detection systems to be used in fusion research. Four types of such detection systems are presented in this article: three diagnostic systems used to measure electric quantities (Rogowski coil, magnetic probe, capacitance probe, neutron counter based on beryllium activation, fast neutron pinhole camera based on small-area BCF-12 plastic scintillation detectors and high-speed four-frame soft X-ray camera with microchannel plate.

  17. Chlamydomonas DYX1C1/PF23 is essential for axonemal assembly and proper morphology of inner dynein arms.

    Directory of Open Access Journals (Sweden)

    Ryosuke Yamamoto

    2017-09-01

    Full Text Available Cytoplasmic assembly of ciliary dyneins, a process known as preassembly, requires numerous non-dynein proteins, but the identities and functions of these proteins are not fully elucidated. Here, we show that the classical Chlamydomonas motility mutant pf23 is defective in the Chlamydomonas homolog of DYX1C1. The pf23 mutant has a 494 bp deletion in the DYX1C1 gene and expresses a shorter DYX1C1 protein in the cytoplasm. Structural analyses, using cryo-ET, reveal that pf23 axonemes lack most of the inner dynein arms. Spectral counting confirms that DYX1C1 is essential for the assembly of the majority of ciliary inner dynein arms (IDA as well as a fraction of the outer dynein arms (ODA. A C-terminal truncation of DYX1C1 shows a reduction in a subset of these ciliary IDAs. Sucrose gradients of cytoplasmic extracts show that preassembled ciliary dyneins are reduced compared to wild-type, which suggests an important role in dynein complex stability. The role of PF23/DYX1C1 remains unknown, but we suggest that DYX1C1 could provide a scaffold for macromolecular assembly.

  18. Redefining the PF06864 Pfam family based on Burkholderia pseudomallei PilO2(Bp S-SAD crystal structure.

    Directory of Open Access Journals (Sweden)

    Patricia Lassaux

    Full Text Available Type IV pili are surface-exposed filaments and bacterial virulence factors, represented by the Tfpa and Tfpb types, which assemble via specific machineries. The Tfpb group is further divided into seven variants, linked to heterogeneity in the assembly machineries. Here we focus on PilO2(Bp, a protein component of the Tfpb R64 thin pilus variant assembly machinery from the pathogen Burkholderia pseudomallei. PilO2(Bp belongs to the PF06864 Pfam family, for which an improved definition is presented based on newly derived Hidden Markov Model (HMM profiles. The 3D structure of the N-terminal domain of PilO2(Bp (N-PilO2(Bp, here reported, is the first structural representative of the PF06864 family. N-PilO2(Bp presents an actin-like ATPase fold that is shown to be present in BfpC, a different variant assembly protein; the new HMM profiles classify BfpC as a PF06864 member. Our results provide structural insight into the PF06864 family and on the Type IV pili assembly machinery.

  19. Activation of Liver AMPK with PF-06409577 Corrects NAFLD and Lowers Cholesterol in Rodent and Primate Preclinical Models.

    Science.gov (United States)

    Esquejo, Ryan M; Salatto, Christopher T; Delmore, Jake; Albuquerque, Bina; Reyes, Allan; Shi, Yuji; Moccia, Rob; Cokorinos, Emily; Peloquin, Matthew; Monetti, Mara; Barricklow, Jason; Bollinger, Eliza; Smith, Brennan K; Day, Emily A; Nguyen, Chuong; Geoghegan, Kieran F; Kreeger, John M; Opsahl, Alan; Ward, Jessica; Kalgutkar, Amit S; Tess, David; Butler, Lynne; Shirai, Norimitsu; Osborne, Timothy F; Steinberg, Gregory R; Birnbaum, Morris J; Cameron, Kimberly O; Miller, Russell A

    2018-04-08

    Dysregulation of hepatic lipid and cholesterol metabolism is a significant contributor to cardiometabolic health, resulting in excessive liver lipid accumulation and ultimately non-alcoholic steatohepatitis (NASH). Therapeutic activators of the AMP-Activated Protein Kinase (AMPK) have been proposed as a treatment for metabolic diseases; we show that the AMPK β1-biased activator PF-06409577 is capable of lowering hepatic and systemic lipid and cholesterol levels in both rodent and monkey preclinical models. PF-06409577 is able to inhibit de novo lipid and cholesterol synthesis pathways, and causes a reduction in hepatic lipids and mRNA expression of markers of hepatic fibrosis. These effects require AMPK activity in the hepatocytes. Treatment of hyperlipidemic rats or cynomolgus monkeys with PF-06409577 for 6weeks resulted in a reduction in circulating cholesterol. Together these data suggest that activation of AMPK β1 complexes with PF-06409577 is capable of impacting multiple facets of liver disease and represents a promising strategy for the treatment of NAFLD and NASH in humans. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

  20. The preparation and properties of a novel electrolyte of electrochemical double layer capacitors based on LiPF6 and acetamide

    International Nuclear Information System (INIS)

    Li Qi; Zuo Xiaoxi; Liu Jiansheng; Xiao Xin; Shu Dong; Nan Junmin

    2011-01-01

    A novel electrolyte applied in electrochemical double-layer capacitors (EDLCs) has been prepared based on lithium hexafluorophosphate (LiPF 6 ) and acetamide and subsequently characterized by differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA), electrochemical techniques and so on. The mixtures of LiPF 6 and acetamide at the molar ratios of 1:4 to 1:6 exist as liquids below 25 °C, which is attributed to the melting point depression of mixture and the coordination of the polar groups (C=O and NH groups) of acetamide with Li + and PF 6 − ions. The strong interaction between LiPF 6 and acetamide results in the rupture of the electrovalent bond of LiPF 6 and the breakage of hydrogen bonds among the acetamide molecules, leading to the formation of a liquid electrolyte. The LiPF 6 /acetamide electrolyte with a molar ratio of 1:5.5 exhibits a 5.2 V electrochemical window and suitable ionic conductivity at room temperature. In particular, the coin-type cells with carbon electrodes and LiPF 6 /acetamide electrolyte possess high thermal stability and electrochemical properties, showing that the as-prepared LiPF 6 /acetamide electrolyte is a promising candidate for EDLCs.

  1. PfPI3K, a Phosphatidylinsoitol-3 kinase in Plasmodium falciparum, is exported to the host erythrocyte and is involved in hemoglobin trafficking

    CSIR Research Space (South Africa)

    Vaid, A

    2010-03-01

    Full Text Available largely unexplored. Our present studies suggest that PfPI3K, a novel phosphatidylinositol-3-kinase (PI3K) in Plasmodium falciparum, is exported to the host erythrocyte by the parasite in an active form. PfPI3K is a versatile enzyme as it can generate...

  2. Advanced Heart Failure

    Science.gov (United States)

    ... Artery Disease Venous Thromboembolism Aortic Aneurysm More Advanced Heart Failure Updated:May 9,2017 When heart failure (HF) ... Making This content was last reviewed May 2017. Heart Failure • Home • About Heart Failure • Causes and Risks for ...

  3. The improvement of the heat transfer coupling between the flow field and the conduction slab for TRAC-PF1

    International Nuclear Information System (INIS)

    Wang, Lang Chen.

    1990-01-01

    A semi-implicit coupling technique (treating only the fluid temperature in the energy source term implicitly) of fluid heat-transfer to conduction slabs is presently used in TRAC-PF1. In this study, a fully implicit coupling scheme between the flow field and conduction slab for the one-dimensional capability of TRAC-PF1 has been developed. The new methods treat the heat-transfer coefficient and wall temperature in the energy source term of both the convection and the conduction equation implicitly. In order to test the accuracy of the standard TRAC coupling method and the new methods used in TRAC-PF1, a series of simple tube experiments were modeled with TRAC-PF1 version 3.9B. Additional studies showed the current TRAC-PF1 convergence variables (pressure fraction change and temperature change) and the current convergence criteria are not appropriate for obtaining an accurate result. Use of these values would produce non-physical double values or would adversely affect results in the reflood calculation. Another study also had been done to determine the importance of each HTC variable on computational accuracy and speed by using the non-linear oterative method. It is found that the heat-transfer correlations have a strong dependency on the local void fraction rather than on fluid velocity, fluid temperature, and wall surface temperature in the film boiling heat-transfer regime. At the beginning of a cooldown transient, the old time void fraction is always higher than the new time void fraction in the same time step. The old time void fraction evaluates a lower heat-transfer coefficient for that cell and initiates cooldown later and more slowly. This then leads to a longer quench time and lowers the fluid temperature and the wall temperature plateau for the downstream control volumes

  4. Evasion of Immunity to Plasmodium falciparum: Rosettes of Blood Group A Impair Recognition of PfEMP1

    Science.gov (United States)

    Moll, Kirsten; Palmkvist, Mia; Ch'ng, Junhong; Kiwuwa, Mpungu Steven; Wahlgren, Mats

    2015-01-01

    The ABO blood group antigens are expressed on erythrocytes but also on endothelial cells, platelets and serum proteins. Notably, the ABO blood group of a malaria patient determines the development of the disease given that blood group O reduces the probability to succumb in severe malaria, compared to individuals of groups A, B or AB. P. falciparum rosetting and sequestration are mediated by PfEMP1, RIFIN and STEVOR, expressed at the surface of the parasitized red blood cell (pRBC). Antibodies to these antigens consequently modify the course of a malaria infection by preventing sequestration and promoting phagocytosis of pRBC. Here we have studied rosetting P. falciparum and present evidence of an immune evasion mechanism not previously recognized. We find the accessibility of antibodies to PfEMP1 at the surface of the pRBC to be reduced when P. falciparum forms rosettes in blood group A RBC, as compared to group O RBC. The pRBC surrounds itself with tightly bound normal RBC that makes PfEMP1 inaccessible to antibodies and clearance by the immune system. Accordingly, pRBC of in vitro cloned P. falciparum devoid of ABO blood group dependent rosetting were equally well detected by anti-PfEMP1 antibodies, independent of the blood group utilized for their propagation. The pathogenic mechanisms underlying the severe forms of malaria may in patients of blood group A depend on the ability of the parasite to mask PfEMP1 from antibody recognition, in so doing evading immune clearance. PMID:26714011

  5. Spatial and temporal activity of the foxtail millet (Setaria italica) seed-specific promoter pF128.

    Science.gov (United States)

    Pan, Yanlin; Ma, Xin; Liang, Hanwen; Zhao, Qian; Zhu, Dengyun; Yu, Jingjuan

    2015-01-01

    pF128 drives GUS specifically expressed in transgenic seeds of foxtail millet and Zea mays with higher activity than the constitutive CaMV35S promoter and the maize seed-specific 19Z promoter. Foxtail millet (Setaria italica), a member of the Poaceae family, is an important food and fodder crop in arid regions. Foxtail millet is an excellent C4 crop model owing to its small genome (~490 Mb), self-pollination and availability of a complete genome sequence. F128 was isolated from a cDNA library of foxtail millet immature seeds. Real-time PCR analysis revealed that F128 mRNA was specifically expressed in immature and mature seeds. The highest F128 mRNA level was observed 5 days after pollination and gradually decreased as the seed matured. Sequence analysis suggested that the protein encoded by F128 is likely a protease inhibitor/seed storage protein/lipid-transfer protein. The 1,053 bp 5' flanking sequence of F128 (pF128) was isolated and fused to the GUS reporter gene. The corresponding vector was then transformed into Arabidopsis thaliana, foxtail millet and Zea mays. GUS analysis revealed that pF128 drove GUS expression efficiently and specifically in the seeds of transgenic Arabidopsis, foxtail millet and Zea mays. GUS activity was also detected in Arabidopsis cotyledons. Activity of pF128 was higher than that observed for the constitutive CaMV35S promoter and the maize seed-specific 19 Zein (19Z) promoter. These results indicate that pF128 is a seed-specific promoter. Its application is expected to be of considerable value in plant genetic engineering.

  6. Immune mediated liver failure

    OpenAIRE

    Wang, Xiaojing; Ning, Qin

    2014-01-01

    Liver failure is a clinical syndrome of various etiologies, manifesting as jaundice, encephalopathy, coagulopathy and circulatory dysfunction, which result in subsequent multiorgan failure. Clinically, liver failure is classified into four categories: acute, subacute, acute-on-chronic and chronic liver failure. Massive hepatocyte death is considered to be the core event in the development of liver failure, which occurs when the extent of hepatocyte death is beyond the liver regenerative capac...

  7. Chronic heart failure

    OpenAIRE

    Hopper, Ingrid; Easton, Kellie

    2017-01-01

    1. The common symptoms and signs of chronic heart failure are dyspnoea, ankle swelling, raised jugular venous pressure and basal crepitations. Other conditions may be confused with chronic heart failure, including dependent oedema or oedema due to renal or hepatic disease. Shortness of breath may be due to respiratory disease or severe anaemia. Heart failure secondary to lung disease (cor pulmonale) should be distinguished from congestive cardiac failure. Heart failure may also present with l...

  8. The failure-tolerant leader.

    Science.gov (United States)

    Farson, Richard; Keyes, Ralph

    2002-08-01

    "The fastest way to succeed," IBM's Thomas Watson, Sr., once said, "is to double your failure rate." In recent years, more and more executives have embraced Watson's point of view, coming to understand what innovators have always known: Failure is a prerequisite to invention. But while companies may grasp the value of making mistakes at the level of corporate practices, they have a harder time accepting the idea at the personal level. People are afraid to fail, and corporate culture reinforces that fear. In this article, psychologist and former Harvard Business School professor Richard Farson and coauthor Ralph Keyes discuss how companies can reduce the fear of miscues. What's crucial is the presence of failure-tolerant leaders--executives who, through their words and actions, help employees overcome their anxieties about making mistakes and, in the process, create a culture of intelligent risk-taking that leads to sustained innovation. Such leaders don't just accept productive failure, they promote it. Drawing from their research in business, politics, sports, and science, the authors identify common practices among failure-tolerant leaders. These leaders break down the social and bureaucratic barriers that separate them from their followers. They engage at a personal level with the people they lead. They avoid giving either praise or criticism, preferring to take a nonjudgmental, analytical posture as they interact with staff. They openly admit their own mistakes rather than trying to cover them up or shifting the blame. And they try to root out the destructive competitiveness built into most organizations. Above all else, failure-tolerant leaders push people to see beyond traditional definitions of success and failure. They know that as long as a person views failure as the opposite of success, rather than its complement, he or she will never be able to take the risks necessary for innovation.

  9. Solution structure of a Plasmodium falciparum AMA-1/MSP 1 chimeric protein vaccine candidate (PfCP-2.9 for malaria

    Directory of Open Access Journals (Sweden)

    Jin Changwen

    2010-03-01

    Full Text Available Abstract Background The Plasmodium falciparum chimeric protein PfCP-2.9 is a promising asexual-stage malaria vaccine evaluated in clinical trials. This chimeric protein consists of two cysteine-rich domains: domain III of the apical membrane antigen 1 (AMA-1 [III] and the C-terminal region of the merozoite surface protein 1 (MSP1-19. It has been reported that the fusion of these two antigens enhanced their immunogenicity and antibody-mediated inhibition of parasite growth in vitro. Methods The 15N-labeled and 13C/15N-labeled PfCP-2.9 was produced in Pichia pastoris for nuclear magnetic resonance (NMR structure analysis. The chemical shift assignments of PfCP-2.9 were compared with those previously reported for the individual domains (i.e., PfAMA-1(III or PfMSP 1-19. The two-dimensional spectra and transverse relaxation rates (R2 of the PfMSP1-19 alone were compared with that of the PfCP-2.9. Results Confident backbone assignments were obtained for 122 out of 241 residues of PfCP-2.9. The assigned residues in PfCP-2.9 were very similar to those previously reported for the individual domains. The conformation of the PfMSP1-19 in different constructs is essentially the same. Comparison of transverse relaxation rates (R2 strongly suggests no weak interaction between the domains. Conclusions These data indicate that the fusion of AMA-1(III and MSP1-19 as chimeric protein did not change their structures, supporting the use of the chimeric protein as a potential malaria vaccine.

  10. Si-semiconductor device failure mechanisms

    International Nuclear Information System (INIS)

    Clauss, H.

    1976-12-01

    This report presents investigations on failure mechanisms that may cause defects during production and operation of silicon semiconductor devices. The failure analysis of aluminium metallization defects covers topics such as step coverage, dissolution pits and electromigration. Furthermore, the generation of process induced lattice defects was investigated. Improved processes avoiding those defects were developed. (orig.) [de

  11. Implications of Infliximab Treatment Failure and Influence of Personalized Treatment on Patient-reported Health-related Quality of Life and Productivity Outcomes in Crohn's Disease

    DEFF Research Database (Denmark)

    Steenholdt, Casper; Brynskov, Jørn; Thomsen, Ole Ø

    2015-01-01

    regimen (n = 36) or personalized treatment defined by IFX and anti-IFX antibodies (n = 33). Health-related quality of life evaluated with the Short Inflammatory Bowel Disease Questionnaire (IBDQ) and productivity evaluated with the Work Productivity and Activity Impairment Questionnaire (WPAI:CD) were...... (increase of median 4, p absenteeism was negligible during the entire study period...

  12. Heart failure - surgeries and devices

    Science.gov (United States)

    ... surgery; HF - surgery; Intra-aortic balloon pumps - heart failure; IABP - heart failure; Catheter based assist devices - heart failure ... problem may cause heart failure or make heart failure worse. Heart valve surgery may be needed to repair or ...

  13. Risk-based decision making to manage water quality failures caused by combined sewer overflows

    Science.gov (United States)

    Sriwastava, A. K.; Torres-Matallana, J. A.; Tait, S.; Schellart, A.

    2017-12-01

    Regulatory authorities set certain environmental permit for water utilities such that the combined sewer overflows (CSO) managed by these companies conform to the regulations. These utility companies face the risk of paying penalty or negative publicity in case they breach the environmental permit. These risks can be addressed by designing appropriate solutions such as investing in additional infrastructure which improve the system capacity and reduce the impact of CSO spills. The performance of these solutions is often estimated using urban drainage models. Hence, any uncertainty in these models can have a significant effect on the decision making process. This study outlines a risk-based decision making approach to address water quality failure caused by CSO spills. A calibrated lumped urban drainage model is used to simulate CSO spill quality in Haute-Sûre catchment in Luxembourg. Uncertainty in rainfall and model parameters is propagated through Monte Carlo simulations to quantify uncertainty in the concentration of ammonia in the CSO spill. A combination of decision alternatives such as the construction of a storage tank at the CSO and the reduction in the flow contribution of catchment surfaces are selected as planning measures to avoid the water quality failure. Failure is defined as exceedance of a concentration-duration based threshold based on Austrian emission standards for ammonia (De Toffol, 2006) with a certain frequency. For each decision alternative, uncertainty quantification results into a probability distribution of the number of annual CSO spill events which exceed the threshold. For each alternative, a buffered failure probability as defined in Rockafellar & Royset (2010), is estimated. Buffered failure probability (pbf) is a conservative estimate of failure probability (pf), however, unlike failure probability, it includes information about the upper tail of the distribution. A pareto-optimal set of solutions is obtained by performing mean

  14. Nutritional, technological and managerial parameters for precision feeding to enhance feed nutrient utilization and productivity in different dairy cattle production systems

    DEFF Research Database (Denmark)

    van Empel, Mireille JM; Makkar, Harinder PS; Dijkstra, Jan

    2016-01-01

    Increased future demand of animal products as well as competition between food, feed and fuel, require efficient utilization of feed resources to strengthen environmental, economic and social sustainability of livestock systems. The objective of this review is to summarize current knowledge...... on precision feeding (PF) and the relevance of PF approaches in dairy cattle production systems in developing countries. The concept of PF aims at achieving balanced nutrition (matching animal requirements with nutrient supply, preferably from locally available feed resources) to improve animal productivity...... and to reduce both the cost of production and environmental pollution. In addition to the supply of proper amounts of nutrients to the dairy cow using various methodologies and tools, approaches that enhance overall nutrient digestion and availability to the animal are also discussed as an integral part of PF...

  15. Complications and fixation techniques of trochanteric fractures with the TARGON(®) PF.

    Science.gov (United States)

    Takigawa, Naohide; Moriuchi, Hiromitsu; Abe, Muneki; Yasui, Kenji; Eshiro, Hisako; Kinoshita, Mitsuo

    2014-01-01

    The purpose of this study was to analyse clinical results of elderly patients with trochanteric fractures that were treated with use of TARGON(®) PF nail. Overall, 494 patients (113 males) were available for this study. On the basis of Jensen classification, there were 76 cases in type I, 164 cases in type II, 70 in type III, 129 in type IV and 55 in type V. (1) Sliding amount of lag screw in relation to the Jensen classification, (2) sliding amount of lag screw according to the Ikuta's classification that categorises the reduction in lateral view in three, Subtype A, Subtype N and Subtype P, (3) sliding amount of lag screw in correlation between Jensen classification and Ikuta's classification and (4) postoperative complications (9 cases; 1.7%) were assessed. Cut-out and back-out cases were seen in 6 cases (1.1%), and these severe complications were evaluated in details. In correlation between Jensen classification and Ikuta's classification, the excessive sliding of lag screw was prominent with the Subtype P, which was preoperatively in Jensen type III or V. Cut-out or back-out cases were caused either from (1) Subtype P that were preoperatively Jensen types III or V, or from (2) the fracture where there was bony defect anteriorly. Therefore, special care must be taken for these types. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Los Alamos County Fire Department LAFD: TA-55 PF-4 Facility Familiarization Tour, OJT 55260

    Energy Technology Data Exchange (ETDEWEB)

    Rutherford, Victor Stephen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-07-13

    Los Alamos National Laboratory (LANL) will conduct familiarization tours for Los Alamos County Fire Department (LAFD) personnel at the Plutonium Facility (PF-4) at Technical Area (TA)-55. These familiarization tours are official LANL business; the purpose of these tours is to orient the firefighters to the facility so that they can respond efficiently and quickly to a variety of emergency situations. This orientation includes the ingress and egress of the area and buildings, layout and organization of the facility, evacuation procedures and assembly points, and areas of concern within the various buildings at the facility. LAFD firefighters have the skills and abilities to perform firefighting operations and other emergency response tasks that cannot be provided by other LANL personnel who have the required clearance level. This handout provides details of the information, along with maps and diagrams, to be presented during the familiarization tours. The handout will be distributed to the trainees at the time of the tour. A corresponding checklist will also be used as guidance during the familiarization tours to ensure that all required information is presented to LAFD personnel.

  17. Studies of plasma interactions with tungsten targets in PF-1000U facility

    Directory of Open Access Journals (Sweden)

    Ladygina Maryna S.

    2016-06-01

    Full Text Available This paper presents results of experimental studies of tungsten samples of 99.95% purity, which were irradiated by intense plasma-ion streams. The behaviour of tungsten, and particularly its structural change induced by high plasma loads, is of great importance for fusion technology. The reported measurements were performed within a modified PF-1000U plasma-focus facility operated at the IFPiLM in Warsaw, Poland. The working gas was pure deuterium. In order to determine the main plasma parameters and to study the behaviour of impurities at different instants of the plasma discharge, the optical emission spectroscopy was used. The dependence of plasma parameters on the initial charging voltage (16, 19 and 21 kV was studied. Detailed optical measurements were performed during interactions of a plasma stream with the tungsten samples placed at the z-axis of the facility, at a distance of 6 cm from the electrode outlets. The recorded spectra showed distinct WI and WII spectral lines. Investigation of a target surface morphology, after its irradiation by intense plasma streams, was performed by means of an optical microscope. The observations revealed that some amounts of the electrodes material (mainly copper were deposited upon the irradiated sample surface. In all the cases, melted zones were observed upon the irradiated target surface, and in experiments performed at the highest charging voltage there were formed some cracks.

  18. Posttest analysis of MIST Test 330302 using TRAC-PF1/MOD1

    International Nuclear Information System (INIS)

    Boyack, B.E.

    1992-09-01

    This report discusses a posttest analysis of Multi-Loop Integral System Test (MIST) 330302 which has been performed using TRAC-PF1/MOD1. This test was one of group performed in the MIST facility to investigate high-pressure injection (HPI)-power-operated relief valve (PORV) cooling, also known as feed-and-bleed cooling. In Test 330302, HPI cooling was delayed 20 min after opening and locking the PORV open to induce extensive system voiding. We have concluded that the TRAC-calculated results are in reasonable overall agreement with the data for Test 330302. All major trends and phenomena were correctly predicted. Differences observed between the measured and calculated results have been traced and related, in part, to deficiencies in our knowledge of the facility configuration and operation. We have identified two models forwhich additional review is appropriate. However, in general, the TRAC closure models and correlations appear to be adequate for the prediction of the phenomena expected to occur during feed-and-bleed transientsin the MIST facility. We believe that the correct conclusions about trends and phenomena will be reached if the code is used in similar applications. Conclusions reached regarding use of the code to calculate similar phenomena in full-size plants (scaling implications) and regulatory implications of this work are also presented

  19. INCLUSION OF THE LATENT PERSONALITY VARIABLE IN MULTINOMIAL LOGIT MODELS USING THE 16PF PSYCHOMETRIC TEST

    Directory of Open Access Journals (Sweden)

    JORGE E. CÓRDOBA MAQUILÓN

    2012-01-01

    Full Text Available Los modelos de demanda de viajes utilizan principalmente los atributos modales y las características socioeconómicas como variables explicativas. También se ha establecido que las actitudes y percepciones influyen en el comportamiento de los usuarios. Sin embargo, las variables psicológicas del individuo condicionan la conducta del usuario. En este estudio se incluyó la variable latente personalidad, en la estimación del modelo híbrido de elección discreta, el cual constituye una buena alternativa para incorporar los efectos de los factores subjetivos. La variable latente personalidad se evaluó con la prueba psicométrica 16PF de validez internacional. El artículo analiza los resultados de la aplicación de este modelo a una población de empleados y docentes universitarios, y también propone un camino para la utilización de pruebas psicométricas en los modelos híbridos de elección discreta. Nuestros resultados muestran que los modelos híbridos que incluyen variables latentes psicológicas son superiores a los modelos tradicionales que ignoran los efectos de la conducta de los usuarios.

  20. RNAdualPF: software to compute the dual partition function with sample applications in molecular evolution theory.

    Science.gov (United States)

    Garcia-Martin, Juan Antonio; Bayegan, Amir H; Dotu, Ivan; Clote, Peter

    2016-10-19

    RNA inverse folding is the problem of finding one or more sequences that fold into a user-specified target structure s 0 , i.e. whose minimum free energy secondary structure is identical to the target s 0 . Here we consider the ensemble of all RNA sequences that have low free energy with respect to a given target s 0 . We introduce the program RNAdualPF, which computes the dual partition function Z ∗ , defined as the sum of Boltzmann factors exp(-E(a,s 0 )/RT) of all RNA nucleotide sequences a compatible with target structure s 0 . Using RNAdualPF, we efficiently sample RNA sequences that approximately fold into s 0 , where additionally the user can specify IUPAC sequence constraints at certain positions, and whether to include dangles (energy terms for stacked, single-stranded nucleotides). Moreover, since we also compute the dual partition function Z ∗ (k) over all sequences having GC-content k, the user can require that all sampled sequences have a precise, specified GC-content. Using Z ∗ , we compute the dual expected energy 〈E ∗ 〉, and use it to show that natural RNAs from the Rfam 12.0 database have higher minimum free energy than expected, thus suggesting that functional RNAs are under evolutionary pressure to be only marginally thermodynamically stable. We show that C. elegans precursor microRNA (pre-miRNA) is significantly non-robust with respect to mutations, by comparing the robustness of each wild type pre-miRNA sequence with 2000 [resp. 500] sequences of the same GC-content generated by RNAdualPF, which approximately [resp. exactly] fold into the wild type target structure. We confirm and strengthen earlier findings that precursor microRNAs and bacterial small noncoding RNAs display plasticity, a measure of structural diversity. We describe RNAdualPF, which rapidly computes the dual partition function Z ∗ and samples sequences having low energy with respect to a target structure, allowing sequence constraints and specified GC

  1. Field failure mechanisms for photovoltaic modules

    Science.gov (United States)

    Dumas, L. N.; Shumka, A.

    1981-01-01

    Beginning in 1976, Department of Energy field centers have installed and monitored a number of field tests and application experiments using current state-of-the-art photovoltaic modules. On-site observations of module physical and electrical degradation, together with in-depth laboratory analysis of failed modules, permits an overall assessment of the nature and causes of early field failures. Data on failure rates are presented, and key failure mechanisms are analyzed with respect to origin, effect, and prospects for correction. It is concluded that all failure modes identified to date are avoidable or controllable through sound design and production practices.

  2. LiPF{sub 6}. Synthesis and stability in EC/DMC and PC/DMC mixtures; LiPF{sub 6}. Synthese et stabilite dans les melanges EC/DMC et PC/DMC

    Energy Technology Data Exchange (ETDEWEB)

    Naejus, R.; Coudert, R.; Lemordant, D. [Laboratoire P.I.M.I.R. EA, Sciences et Techniques, 37 - Tours (France); Willmann, P. [CNES, 31 - Toulouse (France)

    1996-12-31

    Lithium hexa-fluoro-phosphate LiPF{sub 6} is recommended for the replacement of the toxic LiAsF{sub 6} and the explosive perchlorates (like LiClO{sub 4}) in rechargeable lithium electrochemical generators. The aim of this work is to develop a new method of synthesis of this salt and to check its stability with respect to carbonated solvents: ethylene carbonate (EC), propylene carbonate (PC) and dimethyl-carbonate (DMC) in already optimized EC/DMC and PC/DMC binary mixtures. Two methods using HPF{sub 6} are proposed: the first one uses the direct neutralization of this commercial acid by LiOH in aqueous, alcoholic or acetonitrile environment, while in the second one LiPF{sub 6} is obtained from pyridinium hexa-fluoro-phosphate synthesized from HPF{sub 6} using a new and simple protocol. (J.S.) 24 refs.

  3. LiPF{sub 6}. Synthesis and stability in EC/DMC and PC/DMC mixtures; LiPF{sub 6}. Synthese et stabilite dans les melanges EC/DMC et PC/DMC

    Energy Technology Data Exchange (ETDEWEB)

    Naejus, R; Coudert, R; Lemordant, D [Laboratoire P.I.M.I.R. EA, Sciences et Techniques, 37 - Tours (France); Willmann, P [CNES, 31 - Toulouse (France)

    1997-12-31

    Lithium hexa-fluoro-phosphate LiPF{sub 6} is recommended for the replacement of the toxic LiAsF{sub 6} and the explosive perchlorates (like LiClO{sub 4}) in rechargeable lithium electrochemical generators. The aim of this work is to develop a new method of synthesis of this salt and to check its stability with respect to carbonated solvents: ethylene carbonate (EC), propylene carbonate (PC) and dimethyl-carbonate (DMC) in already optimized EC/DMC and PC/DMC binary mixtures. Two methods using HPF{sub 6} are proposed: the first one uses the direct neutralization of this commercial acid by LiOH in aqueous, alcoholic or acetonitrile environment, while in the second one LiPF{sub 6} is obtained from pyridinium hexa-fluoro-phosphate synthesized from HPF{sub 6} using a new and simple protocol. (J.S.) 24 refs.

  4. The Malaria Parasite Cyclin H Homolog PfCyc1 Is Required for Efficient Cytokinesis in Blood-Stage Plasmodium falciparum.

    Science.gov (United States)

    Robbins, Jonathan A; Absalon, Sabrina; Streva, Vincent A; Dvorin, Jeffrey D

    2017-06-13

    All well-studied eukaryotic cell cycles are driven by cyclins, which activate cyclin-dependent kinases (CDKs), and these protein kinase complexes are viable drug targets. The regulatory control of the Plasmodium falciparum cell division cycle remains poorly understood, and the roles of the various CDKs and cyclins remain unclear. The P. falciparum genome contains multiple CDKs, but surprisingly, it does not contain any sequence-identifiable G 1 -, S-, or M-phase cyclins. We demonstrate that P. falciparum Cyc1 (PfCyc1) complements a G 1 cyclin-depleted Saccharomyces cerevisiae strain and confirm that other identified malaria parasite cyclins do not complement this strain. PfCyc1, which has the highest sequence similarity to the conserved cyclin H, cannot complement a temperature-sensitive yeast cyclin H mutant. Coimmunoprecipitation of PfCyc1 from P. falciparum parasites identifies PfMAT1 and PfMRK as specific interaction partners and does not identify PfPK5 or other CDKs. We then generate an endogenous conditional allele of PfCyc1 in blood-stage P. falciparum using a destabilization domain (DD) approach and find that PfCyc1 is essential for blood-stage proliferation. PfCyc1 knockdown does not impede nuclear division, but it prevents proper cytokinesis. Thus, we demonstrate that PfCyc1 has a functional divergence from bioinformatic predictions, suggesting that the malaria parasite cell division cycle has evolved to use evolutionarily conserved proteins in functionally novel ways. IMPORTANCE Human infection by the eukaryotic parasite Plasmodium falciparum causes malaria. Most well-studied eukaryotic cell cycles are driven by cyclins, which activate cyclin-dependent kinases (CDKs) to promote essential cell division processes. Remarkably, there are no identifiable cyclins that are predicted to control the cell cycle in the malaria parasite genome. Thus, our knowledge regarding the basic mechanisms of the malaria parasite cell cycle remains unsatisfactory. We

  5. Investigation on Polylactide (PLA)/Poly(butylene adipate-co-terephthalate) (PBAT)/Bark Flour of Plane Tree (PF) Eco-Composites

    Science.gov (United States)

    Dou, Qiang; Cai, Jun

    2016-01-01

    Polylactide (PLA)/poly(butylene adipate-co-terephthalate) (PBAT)/bark flour of plane tree (PF) eco-composites were prepared via melt blending. The morphologies, mechanical properties, crystal structures and melting and crystallization behaviors of the eco-composites were investigated by means of scanning electron microscopy (SEM), mechanical tests, polarized light microscopy (PLM), wide angle X-ray diffraction (WAXD) and differential scanning calorimetry (DSC), respectively. It is shown that the interfacial adhesion between PLA matrix and PF is weak and the mechanical properties of PLA/PF eco-composites are poor. The titanate treatment improves the adhesion between the matrix and the filler and enhances the stiffness of the eco-composites. The toughness is improved by PBAT and ductile fractured surfaces can be found. The spherulitic size of PLA is decreased by the addition of PF. The α crystalline form of PLA remains in the composites. Compared with PF, T-PF (PF treated by a titanate coupling agent) and PBAT have negative effects on the crystallization of PLA. PMID:28773515

  6. Post-test analysis of semiscale large-break test S-06-3 using TRAC-PF1

    International Nuclear Information System (INIS)

    Boyack, B.E.

    1982-01-01

    The Transient Reactor Analysis Code (TRAC) is an advanced systems code for light-water-reactor accident analysis. The code was developed originally to analyze large-break loss-of-coolant accidents (LOCAs) and running time was not a primary development criterion. TRAC-PF1 was developed because increased application of the code to long transients such as small-break LOCAs required a faster-running code version. Although developed for long transients, its performance on large-break transients is still important. This paper assesses the ability of TRAC-PF1 to predict large-break-LOCA Test S-06-3 conducted in the Semiscale Mod-1 facility

  7. Dependence of CIT [Compact Ignition Tokamak] PF [poloidal field] coil currents on profile and shape parameters using the Control Matrix

    International Nuclear Information System (INIS)

    Strickler, D.J.; Peng, Y-K.M.; Jardin, S.C.; Pomphrey, N.

    1990-01-01

    The plasma shaping flexibility of the Compact Ignition Tokamak (CIT) poloidal field (PF) coil set is demonstrated through MHD equilibrium calculations of optimal PF coil current distributions and their variation with poloidal beta, internal inductance, plasma 95% elongation, and 95% triangularity. Calculations of the magnetic stored energy are used to compare solutions associated with various plasma parameters. The Control Matrix (CM) equilibrium code, together with the nonlinear equation and numerical optimization software packages HYBRD, and VMCON, respectively, are used to find equilibrium coil current distributions for fixed divertor geometry, volt-seconds, and plasma profiles in order to isolate the dependence on individual parameters. A reference equilibrium and coil current distribution are chosen, and correction currents dI are determined using the CM equilibrium method to obtain other specified plasma shapes. The reference equilibrium is the κ = 2 divertor at beginning of flattop (BOFT) with a minimum stored energy solution for the coil current distribution. The pressure profile function is fixed

  8. Acute liver failure

    DEFF Research Database (Denmark)

    Larsen, Fin Stolze; Bjerring, Peter Nissen

    2011-01-01

    Acute liver failure (ALF) results in a multitude of serious complications that often lead to multi-organ failure. This brief review focuses on the pathophysiological processes in ALF and how to manage these.......Acute liver failure (ALF) results in a multitude of serious complications that often lead to multi-organ failure. This brief review focuses on the pathophysiological processes in ALF and how to manage these....

  9. Timing analysis of PWR fuel pin failures

    International Nuclear Information System (INIS)

    Jones, K.R.; Wade, N.L.; Katsma, K.R.; Siefken, L.J.; Straka, M.

    1992-09-01

    Research has been conducted to develop and demonstrate a methodology for calculation of the time interval between receipt of the containment isolation signals and the first fuel pin failure for loss-of-coolant accidents (LOCAs). Demonstration calculations were performed for a Babcock and Wilcox (B ampersand W) design (Oconee) and a Westinghouse (W) four-loop design (Seabrook). Sensitivity studies were performed to assess the impacts of fuel pin bumup, axial peaking factor, break size, emergency core cooling system availability, and main coolant pump trip on these times. The analysis was performed using the following codes: FRAPCON-2, for the calculation of steady-state fuel behavior; SCDAP/RELAP5/MOD3 and TRACPF1/MOD1, for the calculation of the transient thermal-hydraulic conditions in the reactor system; and FRAP-T6, for the calculation of transient fuel behavior. In addition to the calculation of fuel pin failure timing, this analysis provides a comparison of the predicted results of SCDAP/RELAP5/MOD3 and TRAC-PFL/MOD1 for large-break LOCA analysis. Using SCDAP/RELAP5/MOD3 thermal-hydraulic data, the shortest time intervals calculated between initiation of containment isolation and fuel pin failure are 10.4 seconds and 19.1 seconds for the B ampersand W and W plants, respectively. Using data generated by TRAC-PF1/MOD1, the shortest intervals are 10.3 seconds and 29.1 seconds for the B ampersand W and W plants, respectively. These intervals are for a double-ended, offset-shear, cold leg break, using the technical specification maximum peaking factor and applied to fuel with maximum design bumup. Using peaking factors commensurate widi actual bumups would result in longer intervals for both reactor designs. This document also contains appendices A through J of this report

  10. Remote replacement of TF [toroidal field] and PF [poloidal field] coils for the compact ignition tokamak

    International Nuclear Information System (INIS)

    Macdonald, D.; Watkin, D.C.; Hollis, M.J.; DePew, R.E.; Kuban, D.P.

    1990-01-01

    The use of deuterium-tritium fuel in the Compact Ignition Tokamak will require applying remote handling technology for ex-vessel maintenance and replacement of machine components. Highly activated and contaminated components of the fusion devices auxiliary systems, such as diagnostics and RF heating, must be replaced using remotely operated maintenance equipment in the test cell. In-vessel remote maintenance included replacement of divertor and first wall hardware, faraday shields, and for an in-vessel inspection system. Provision for remote replacement of a vacuum vessel sector, toroidal field coil or poloidal field ring coil was not included in the project baseline. As a result of recent coil failures experienced at a number of facilities, the CIT project decided to reconsider the question of remote recovery from a coil failure and, in January of 1990, initiated a coil replacement study. This study focused on the technical requirements and impact on fusion machine design associated with remote recovery from any coil failure

  11. Conductivity and thermopower studies of bis-tetramethyltetraselenafulvalenium hexafluorophosphide, bis-tetramethyltetrathiafulvalenium hexafluorophosphide, and their solid solutions, (TMTSF1-xTMTTFx)2PF6

    DEFF Research Database (Denmark)

    Mortensen, Kell; Engler, E. M.

    1984-01-01

    The conductivity σ and the thermoelectric power S have been studied experimentally on the organic conductors bis-tetramethyltetraselenafulvalenium hexafluorophosphide [(TMTSF)2PF6] and bis-tetramethyltetrathiafulvalenium hexafluorophosphide [(TMTTF)2PF6] and their solid solutions: (TMTSF1-xTMTTFx)2......PF6. Dramatic effects are seen in σ already when dilute concentrations of TMTTF molecules are introduced in the TMTSF chains, and for x=0.1, σ shows generally activated behavior. The thermopower, on the contrary, remains basically unaffected for x at least as large as 0.25. These unusual findings...

  12. Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism

    Energy Technology Data Exchange (ETDEWEB)

    Chiu, Hsiu-Ju; Bakolitsa, Constantina; Skerra, Arne; Lomize, Andrei; Carlton, Dennis; Miller, Mitchell D.; Krishna, S. Sri; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Clayton, Thomas; Deller, Marc C.; Duan, Lian; Feuerhelm, Julie; Grant, Joanna C.; Grzechnik, Slawomir K.; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Kozbial, Piotr; Kumar, Abhinav; Marciano, David; McMullan, Daniel; Morse, Andrew T.; Nigoghossian, Edward; Okach, Linda; Paulsen, Jessica; Reyes, Ron; Rife, Christopher L.; van den Bedem, Henry; Weekes, Dana; Xu, Qingping; Hodgson, Keith O.; Wooley, John; Elsliger, Marc-Andre; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A. (SLAC); (Michigan); (TU Munchen)

    2015-10-15

    The first structural representative of the domain of unknown function DUF2006 family, also known as Pfam family PF09410, comprises a lipocalin-like fold with domain duplication. The finding of the calycin signature in the N-terminal domain, combined with remote sequence similarity to two other protein families (PF07143 and PF08622) implicated in isoprenoid metabolism and the oxidative stress response, support an involvement in lipid metabolism. Clusters of conserved residues that interact with ligand mimetics suggest that the binding and regulation sites map to the N-terminal domain and to the interdomain interface, respectively.

  13. PF-1355, a mechanism-based myeloperoxidase inhibitor, prevents immune complex vasculitis and anti-glomerular basement membrane glomerulonephritis.

    Science.gov (United States)

    Zheng, Wei; Warner, Roscoe; Ruggeri, Roger; Su, Chunyan; Cortes, Christian; Skoura, Athanasia; Ward, Jessica; Ahn, Kay; Kalgutkar, Amit; Sun, Dexue; Maurer, Tristan S; Bonin, Paul D; Okerberg, Carlin; Bobrowski, Walter; Kawabe, Thomas; Zhang, Yanwei; Coskran, Timothy; Bell, Sammy; Kapoor, Bhupesh; Johnson, Kent; Buckbinder, Leonard

    2015-05-01

    Small vessel vasculitis is a life-threatening condition and patients typically present with renal and pulmonary injury. Disease pathogenesis is associated with neutrophil accumulation, activation, and oxidative damage, the latter being driven in large part by myeloperoxidase (MPO), which generates hypochlorous acid among other oxidants. MPO has been associated with vasculitis, disseminated vascular inflammation typically involving pulmonary and renal microvasculature and often resulting in critical consequences. MPO contributes to vascular injury by 1) catabolizing nitric oxide, impairing vasomotor function; 2) causing oxidative damage to lipoproteins and endothelial cells, leading to atherosclerosis; and 3) stimulating formation of neutrophil extracellular traps, resulting in vessel occlusion and thrombosis. Here we report a selective 2-thiouracil mechanism-based MPO inhibitor (PF-1355 [2-(6-(2,5-dimethoxyphenyl)-4-oxo-2-thioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamide) and demonstrate that MPO is a critical mediator of vasculitis in mouse disease models. A pharmacokinetic/pharmacodynamic response model of PF-1355 exposure in relation with MPO activity was derived from mouse peritonitis. The contribution of MPO activity to vasculitis was then examined in an immune complex model of pulmonary disease. Oral administration of PF-1355 reduced plasma MPO activity, vascular edema, neutrophil recruitment, and elevated circulating cytokines. In a model of anti-glomerular basement membrane disease, formerly known as Goodpasture disease, albuminuria and chronic renal dysfunction were completely suppressed by PF-1355 treatment. This study shows that MPO activity is critical in driving immune complex vasculitis and provides confidence in testing the hypothesis that MPO inhibition will provide benefit in treating human vasculitic diseases. Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics.

  14. Efficacy of Pseudomonas fluorescens (Pf-CL145A) spray dried powder for controlling zebra mussels adhering to test substrates

    Science.gov (United States)

    Luoma, James A.; Severson, Todd J.; Weber, Kerry L.; Mayer, Denise A.

    2015-01-01

    A mobile bioassay trailer was used to assess the efficacy of Pseudomonas fluorescens (Pf-CL145A) spray dried powder (SDP) formulation for controlling zebra mussels (Dreissena polymorpha) from two midwestern lakes: Lake Carlos (Alexandria, Minnesota) and Shawano Lake (Shawano, Wisconsin). The effects of SDP exposure concentration and exposure duration on zebra mussel survival were evaluated along with the evaluation of a benthic injection application technique to reduce the amount of SDP required to induce zebra mortality.

  15. Understanding the thermal instability of fluoroethylene carbonate in LiPF6-based electrolytes for lithium ion batteries

    International Nuclear Information System (INIS)

    Kim, Koeun; Park, Inbok; Ha, Se-Young; Kim, Yeonkyoung; Woo, Myung-Heuio; Jeong, Myung-Hwan; Shin, Woo Cheol; Ue, Makoto; Hong, Sung You; Choi, Nam-Soon

    2017-01-01

    Highlights: • The FEC in LiPF 6 -based electrolytes thermally decomposes at elevated temperatures. • Lewis acids in the electrolyte promote de-fluorination of the FEC to form HF. • The HF causes the SEI destruction and severe metal ion dissolution from the cathode. - Abstract: The cycling and storage performances of LiCoO 2 (LCO)-LiNi 0.5 Co 0.2 Mn 0.3 O 2 (NCM)/pitch-coated silicon alloy-graphite (Si-C) full cells with ethylene carbonate (EC)–based and fluoroethylene carbonate (FEC)–based electrolytes are investigated at elevated temperatures. Excess FEC (used as a co-solvent in LiPF 6 -based electrolytes), which is not completely consumed during the formation of the solid electrolyte interphase (SEI) layer on the electrodes, is prone to defluorination in the presence of Lewis acids such as PF 5 ; this reaction can generate unwanted HF and various acids (H 3 OPF 6 , HPO 2 F 2 , H 2 PO 3 F, H 3 PO 4 ) at elevated temperatures. Our investigation reveals that the HF and acid compounds that are formed by FEC decomposition causes significant dissolution of transition metal ions (from the LCO-NCM cathode) into the electrolyte at elevated temperatures; as a result, the reversible capacity of the full cells reduces because of the deposition of the dissolved metal ions onto the anode. Moreover, we demonstrate possible mechanisms that account for the thermal instability of FEC in LiPF 6 -based electrolytes at elevated temperatures using model experiments.

  16. In Support of Failure

    Science.gov (United States)

    Carr, Allison

    2013-01-01

    In this essay, I propose a concerted effort to begin devising a theory and pedagogy of failure. I review the discourse of failure in Western culture as well as in composition pedagogy, ultimately suggesting that failure is not simply a judgement or indication of rank but is a relational, affect-bearing concept with tremendous relevance to…

  17. Product Failures in Respirators and Consumables: Analysis of Field Safety Notices of 2005-2013 Publicized by the Federal Institute for Drugs and Medical Devices in Germany.

    Science.gov (United States)

    Hannig, Jürgen; Siekmeier, Rüdiger

    2015-01-01

    The current European system governed by the three EC directives 93/42/EEC (Medical Device Directive), 98/79/EC (In-Vitro Diagnostic Directive) and 90/385/EEC (Active Implantable Medical Device Directive) regulates marketing and post-market surveillance of medical devices in the European Economic Area (EEA). In cases of incidents raising the field safety corrective actions (FSCA), manufacturers have to inform the responsible Competent Authority (CA; in Germany this is the Federal Institute for Drugs and Medical Devices, BfArM) and the public by field safety notices (FSN). In this study we analyzed FSN of respirators and consumables directly required for their function, whereas devices for anesthesia and gas delivery were excluded. FSCA and FSN of 2005-2013 publicized by BfArM for the included products were analyzed with respect to the MEDDEV 2.12-1 rev. 8. In total, 60 FSCA were publicized. German and English FSN were found in 59/53 cases, respectively. FSN were clearly characterized as FSN in 44/38 cases and declaration of the type of action in 45/44 cases, respectively. Product names were provided in all cases. Lot numbers or other information for product characterization were available in 7/7 and 43/40 cases, respectively. Detailed information regarding FSCA and product malfunction was found in all cases. Information on product related risks with previous use of affected devices was provided in 42/38 cases. In 53/53 cases manufacturers provided information to mitigate product related risks. Requests to pass FSN to persons needing awareness in the organization were found in 27/24 cases. Contact data were provided in 53/48 cases, respectively. Confirmation that a CA was informed was found in 28/26 cases and in 19/15 cases a customer confirmation was included. The identified risks were: total loss of function (19/16), short circuit (1/1) and burn (3/3), and inhalation of foreign particles (1/1) which might cause severe risk to patients and users. The most frequent

  18. Electrochemical investigation of Li-Al anodes in oligo(ethylene glycol) dimethyl ether/LiPF6

    International Nuclear Information System (INIS)

    Zhou, Y.; Wang, X.; Lee, H.; Nam, K.; Haas, O.

    2011-01-01

    LiPF 6 dissolved in oligo(ethylene glycol) dimethyl ether with a molecular weight 5 g mol -1 was investigated as a new electrolyte (OEGDME5, 1 M LiPF 6 ) for metal deposition and battery applications. At 25 C a conductivity of .48 x 1 -3 S cm -1 was obtained and at 85 C, 3.78 x 1 -3 S cm -1 . The apparent activation barrier for ionic transport was evaluated to be 3.7 kJ mol -1 . OEGDME5, 1 M LiPF 6 allows operating temperature above 1 C with very attractive conductivity. The electrolyte shows excellent performance at negative and positive potentials. With this investigation, we report experimental results obtained with aluminum electrodes using this electrolyte. At low current densities lithium ion reduction and re-oxidation can be achieved on aluminum electrodes at potentials about 28 mV more positive than on lithium electrodes. In situ X-ray diffraction measurements collected during electrochemical lithium deposition on aluminum electrodes show that the shift to positive potentials is due to the negative Gibbs free energy change of the Li-Al alloy formation reaction.

  19. Conceptual design of PF coil system and operation scenario on inductively-operated day-long pulsed tokamak reactor

    International Nuclear Information System (INIS)

    Wang, J.F.; Yamamoto, T.; Ogawa, Y.

    1994-01-01

    It is said that disadvantages of pulsed operation in tokamak fusion reactor are fatigue problem of structural materials and an introduction of energy storage System to compensate the power during the dwell time. To overcome theses disadvantages the authors have designed an inductively-operated ultralong pulsed tokamak called (IDLT) reactor where plasma with a major radius of 10 m are employed so as to provide a magnetic flux necessary to sustain a plasma current inductively during 10 hours or more. This makes it possible to reduce the total cycle number to be around 10 4 during the life of the fusion plant. In pulsed operation reactors the shorter dwell time with a quick start-up and shut down of plasma is very convenient to realize a high availability of the power plant, but it will induce more severe conditions for the hardware design. The authors assumed the dwell time of 5∼10 minutes and analyzed the feasibility of plasma operation scenario for IDLT reactor, especially paying much attention to PF coil system. The stored energy of PF coil system becomes ∼100 GJ, which is comparable with that of toroidal field coil system. When the plasma current of 14 MA is ramped up with a time of 100 seconds, it is found that the maximum capacity of 1 GW is necessary for PF coil power supply. Engineering issues related with AC/hysterisis loss should be carefully examined

  20. Valproic Acid as a Potential Inhibitor of Plasmodium falciparum Histone Deacetylase 1 (PfHDAC1: An in Silico Approach

    Directory of Open Access Journals (Sweden)

    Mohamed A. Abdallah Elbadawi

    2015-02-01

    Full Text Available A new Plasmodium falciparum histone deacetylase1 (PfHDAC1 homology model was built based on the highest sequence identity available template human histone deacetylase 2 structure. The generated model was carefully evaluated for stereochemical accuracy, folding correctness and overall structure quality. All evaluations were acceptable and consistent. Docking a group of hydroxamic acid histone deacetylase inhibitors and valproic acid has shown binding poses that agree well with inhibitor-bound histone deacetylase-solved structural interactions. Docking affinity dG scores were in agreement with available experimental binding affinities. Further, enzyme-ligand complex stability and reliability were investigated by running 5-nanosecond molecular dynamics simulations. Thorough analysis of the simulation trajectories has shown that enzyme-ligand complexes were stable during the simulation period. Interestingly, the calculated theoretical binding energies of the docked hydroxamic acid inhibitors have shown that the model can discriminate between strong and weaker inhibitors and agrees well with the experimental affinities reported in the literature. The model and the docking methodology can be used in screening virtual libraries for PfHDAC1 inhibitors, since the docking scores have ranked ligands in accordance with experimental binding affinities. Valproic acid calculated theoretical binding energy suggests that it may inhibit PfHDAC1.

  1. Cycle stability of the electrochemical capacitors patterned with vertically aligned carbon nanotubes in an LiPF6-based electrolyte.

    Science.gov (United States)

    Chiou, Yi-Deng; Tsai, Dah-Shyang; Lam, Hoa Hung; Chang, Chuan-hua; Lee, Kuei-Yi; Huang, Ying-Sheng

    2013-09-07

    The miniature ultracapacitors, with interdigitated electrodes of vertically aligned carbon nanotubes (VACNTs) and an inter-electrode gap of 20 μm, have been prepared in the LiPF6 organic electrolyte with and without PVdF-HFP gel. PVdF-HFP between two opposing electrodes enhances the device reliability, but lessens its power performance because of the extra diffusion resistance. Also noteworthy are the gel influences on the cycle stability. When the applied voltage is 2.0 or 2.5 V, both the LiPF6 and the gel capacitors exhibit excellent stability, typified by a retention ratio of ≥95% after 10,000 cycles. Their coulombic efficiencies quickly rise up, and hold steady at 100%. Nonetheless, when the applied voltage is 3.5 or 4.0 V, the cycle stability deteriorates, since the negative electrode potential descends below 0.9 V (vs. Li), leading to electrolyte decomposition and SEI formation. For the LiPF6 capacitor, its retention ratio could be around 60% after 10,000 cycles and the coulombic efficiency of 100% is difficult to reach throughout its cycle life. On the other hand, the gel capacitor cycles energy with a much higher retention ratio, >80% after 10,000 cycles, and a better coulombic efficiency, even though electrolyte decomposition still occurs. We attribute the superior stability of the gel capacitor to its extra diffusion resistance which slows down the performance deterioration.

  2. TRAC-PF1/MOD3 calculations of Savannah River Laboratory Rig FA single-annulus heated experiments

    International Nuclear Information System (INIS)

    Fischer, S.R.; McDaniel, C.K.

    1992-01-01

    This paper presents the results of TRAC-PF1/MOD3 benchmarks of the Rig FA experiments performed at the Savannah River Laboratory to simulate prototypic reactor fuel assembly behavior over a range of fluid conditions typical of the emergency cooling system (ECS) phase of a loss-of-coolant accident (LOCA). The primary purpose of this work was to use the SRL Rig FA tests to qualify the TRAC-PF1/MOD3 computer code and models for computing Mark-22 fuel assembly LOCA/ECS power limits. This qualification effort was part of a larger effort undertaken by the Los Alamos National Laboratory for the US Department of Energy to independently confirm power limits for the Savannah River Site K Reactor. The results of this benchmark effort as discussed in this paper demonstrate that TRAC/PF1/MOD3 coupled with proper modeling is capable of simulating thermal-hydraulic phenomena typical of that encountered in Mark-22 fuel assembly during LOCA/ECS conditions

  3. Progress in mass- and energy- analysis of ion beams emitted from RPI and PF-type discharges

    International Nuclear Information System (INIS)

    Czaus, K.; Skladnik-Sadowska, E.; Kwiatkowski, R.; Malinowski, K.; Zebrowski, J.; Sadowski, M.J.

    2013-01-01

    The paper describes progress in studies of ion beams generated and accelerated within RPI (Rod Plasma Injector) and PF (Plasma-Focus) devices. To perform mass- and energy-analysis of such ion beams the use was made of different mass-spectrometers of the Thomson type. Ion measurements in RPI-IBIS facility were first performed with a big Thomson analyzer placed outside the vacuum chamber. To perform ion measurements near the electrodes two other Thomson analyzers were constructed at IPJ (now NCBJ). A smaller analyzer was applied in a large PF-1000 facility at IFPiLM, and time-integrated ion parabolas were recorded on nuclear track detectors. That analyzer was also adopted for time-resolved measurements by means of miniature scintillation detectors located along the deuteron parabola and it was used in the RPI-IBIS facility. Recently the analyzer construction was modernized to ensure differential pumping of the inlet system, and it was used for measurements within PF-360 and RPI-IBIS facilities.

  4. A portion of the Pf155/RESA antigen of Plasmodium falciparum is accessible on the surface of infected erythrocytes

    International Nuclear Information System (INIS)

    Saul, A.; Maloy, W.L.; Howard, R.J.; Rock, E.P.

    1988-01-01

    An investigation of antigens accessible to lactoperoxidase-catalysed cell surface iodination on intact Plasmodium falciparum-infected red blood cells (RBC) has identified a 125 I-labelled antigen with an apparent size of about 155 kD. This labelled protein was specifically immunoprecipitated by the following antibodies: a rabbit antiserum and a mouse monoclonal antibody raised against a synthetic peptide comprising the 3',8-mer repeat EENVEHDA of the Pf155/RESA protein; a rabbit antiserum raised against a synthetic octapeptide comprising two copies of the 3',4-mer repeat EENV of the Pf155/RESA protein; and rabbit antisera against another synthetic peptide C(MYSNNNVED) 2 . The last antibody shows a strong reaction in asexual blood state parasites with the Pf155/RESA antigen. While this antigen has been described previously as a submembrane component of the outer membrane of infected RBC, this report shows that at least part of it is accessible to the surface of both ring and late trophozoite-infected erythrocytes. 21 refs., 4 figs

  5. The failure of earthquake failure models

    Science.gov (United States)

    Gomberg, J.

    2001-01-01

    In this study I show that simple heuristic models and numerical calculations suggest that an entire class of commonly invoked models of earthquake failure processes cannot explain triggering of seismicity by transient or "dynamic" stress changes, such as stress changes associated with passing seismic waves. The models of this class have the common feature that the physical property characterizing failure increases at an accelerating rate when a fault is loaded (stressed) at a constant rate. Examples include models that invoke rate state friction or subcritical crack growth, in which the properties characterizing failure are slip or crack length, respectively. Failure occurs when the rate at which these grow accelerates to values exceeding some critical threshold. These accelerating failure models do not predict the finite durations of dynamically triggered earthquake sequences (e.g., at aftershock or remote distances). Some of the failure models belonging to this class have been used to explain static stress triggering of aftershocks. This may imply that the physical processes underlying dynamic triggering differs or that currently applied models of static triggering require modification. If the former is the case, we might appeal to physical mechanisms relying on oscillatory deformations such as compaction of saturated fault gouge leading to pore pressure increase, or cyclic fatigue. However, if dynamic and static triggering mechanisms differ, one still needs to ask why static triggering models that neglect these dynamic mechanisms appear to explain many observations. If the static and dynamic triggering mechanisms are the same, perhaps assumptions about accelerating failure and/or that triggering advances the failure times of a population of inevitable earthquakes are incorrect.

  6. Insights into the failure of the potential, neutral myocardial imaging agent TcN-NOET: physicochemical identification of by-products and degradation species

    International Nuclear Information System (INIS)

    Tisato, Francesco; Bolzati, Cristina; Refosco, Fiorenzo; Porchia, Marina; Seraglia, Roberta; Carta, Davide; Pasqualini, Roberto

    2012-01-01

    Introduction: The neutral complex [ 99m Tc(N)(NOEt) 2 ], often referred to as TcN-NOET [NOEt=N-ethoxy,N-ethyldithiocarbamate(1−)], was proposed several years ago as a myocardial imaging agent. Despite some favorable clinical properties evidenced during phase I and phase II studies, the overall results of the European and American phase III clinical studies have been judged insufficient for a successful approval process by the regulatory agencies. Methods: Non-carrier-added and carrier-added experiments using short-lived 99m Tc and long-lived 99g Tc have been utilized to prepare a series of bis-substituted [Tc(N)(DTC) 2 ] complexes [DTC=dithiocarbamate(1−)]. They have been purified by means of chromatographic techniques (high-performance liquid chromatography and thin-layer chromatography) and identified via double detection (UV-vis and radiometry) by comparison with authenticated samples of 99g Tc compounds prepared by conventional coordination chemistry procedures. Results: The molecular structure of the lipophilic, neutral complex cis-[Tc(N)(NOEt) 2 ] has been assigned by comparison with similar nitrido-Tc(V) complexes already reported in the literature. Novel bis-substituted nitrido-Tc complexes containing hydrolyzed portions of coordinated NOEt, namely, N-ethyldithiocarbamate [NHEt(1−)] and N-hydroxy, N-ethyldithiocarbamate [NOHEt(1−)], have been prepared and characterized by means of multinuclear nuclear magnetic resonance spectroscopy and mass spectrometry. Conclusions: Despite the identification of these “hydrolyzed” species, it is still unclear whether the failure to reach the clinical goal of the perfusion tracer [ 99m Tc(N)(NOEt) 2 ] is related to the degradation processes evidenced in this study or is the result of the mediocre imaging properties of the tracer.

  7. Insights into the failure of the potential, neutral myocardial imaging agent TcN-NOET: physicochemical identification of by-products and degradation species.

    Science.gov (United States)

    Tisato, Francesco; Bolzati, Cristina; Refosco, Fiorenzo; Porchia, Marina; Seraglia, Roberta; Carta, Davide; Pasqualini, Roberto

    2012-04-01

    The neutral complex [(99m)Tc(N)(NOEt)(2)], often referred to as TcN-NOET [NOEt=N-ethoxy,N-ethyldithiocarbamate(1-)], was proposed several years ago as a myocardial imaging agent. Despite some favorable clinical properties evidenced during phase I and phase II studies, the overall results of the European and American phase III clinical studies have been judged insufficient for a successful approval process by the regulatory agencies. Non-carrier-added and carrier-added experiments using short-lived (99m)Tc and long-lived (99g)Tc have been utilized to prepare a series of bis-substituted [Tc(N)(DTC)(2)] complexes [DTC=dithiocarbamate(1-)]. They have been purified by means of chromatographic techniques (high-performance liquid chromatography and thin-layer chromatography) and identified via double detection (UV-vis and radiometry) by comparison with authenticated samples of (99g)Tc compounds prepared by conventional coordination chemistry procedures. The molecular structure of the lipophilic, neutral complex cis-[Tc(N)(NOEt)(2)] has been assigned by comparison with similar nitrido-Tc(V) complexes already reported in the literature. Novel bis-substituted nitrido-Tc complexes containing hydrolyzed portions of coordinated NOEt, namely, N-ethyldithiocarbamate [NHEt(1-)] and N-hydroxy, N-ethyldithiocarbamate [NOHEt(1-)], have been prepared and characterized by means of multinuclear nuclear magnetic resonance spectroscopy and mass spectrometry. Despite the identification of these "hydrolyzed" species, it is still unclear whether the failure to reach the clinical goal of the perfusion tracer [(99m)Tc(N)(NOEt)(2)] is related to the degradation processes evidenced in this study or is the result of the mediocre imaging properties of the tracer. Copyright © 2012 Elsevier Inc. All rights reserved.

  8. Modelling of coupled self-actuating safety, relief and damped check valve systems with the codes TRAC-PF1 and ROLAST

    International Nuclear Information System (INIS)

    Neumann, U.; Puzalowski, R.; Grimm, I.

    1985-01-01

    Numerical valve models for simulation of selfactuating safety valves and damped check valves are introduced for the computer programs TRAC-PF1 and ROLAST. As examples of application post-test calculations and stability analysis are given. (orig.)

  9. Antioxidant activity and sensory characteristics of Maillard reaction products derived from different peptide fractions of soybean meal hydrolysate.

    Science.gov (United States)

    Yu, Min; He, Shudong; Tang, Mingming; Zhang, Zuoyong; Zhu, Yongsheng; Sun, Hanju

    2018-03-15

    Four peptide fractions PF1 (>5;kDa), PF2 (3-5;kDa), PF3 (1-3;kDa), PF4 (Maillard reaction products (MRPF1, MRPF2, MRPF3 and MRPF4) were evaluated, respectively. Peptides with low molecular weight showed higher contribution to the changes of pH, colour and browning intensity during Maillard reaction. The DPPH radical-scavenging activity of PF4 was significantly improved after Maillard reaction. Aroma volatiles and PLSR analysis suggested MRPF3 had the best sensory characteristics with higher contents of umami amino acids and lower of bitter amino acids, therefore it could be deduced that the umami and meaty characteristics were correlated with the peptides of 1-3;kDa. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Dose-Related Target Occupancy and Effects on Circuitry, Behavior, and Neuroplasticity of the Glycine Transporter-1 Inhibitor PF-03463275 in Healthy and Schizophrenia Subjects.

    Science.gov (United States)

    D'Souza, Deepak Cyril; Carson, Richard E; Driesen, Naomi; Johannesen, Jason; Ranganathan, Mohini; Krystal, John H

    2018-01-31

    Glycine transporter-1 (GlyT1) inhibitors may ameliorate cognitive impairments associated with schizophrenia. The dose-related occupancy and target engagement of the GlyT1 inhibitor PF-03463275 were studied to inform optimal dose selection for a clinical trial for cognitive impairments associated with schizophrenia. In substudy 1, the effects of PF-03463275 (10, 20, and 40 mg twice a day) on occupancy of GlyT1 were tested using positron emission tomography and 18 F-MK-6577, and visual long-term potentiation (LTP) in schizophrenia patients (SZs) and healthy control subjects. Furthermore, the capacity of PF-03463275 to attenuate ketamine-induced disruption of working memory-related activation of a "working memory" circuit was tested only in healthy control subjects using functional magnetic resonance imaging. Subsequently, the effects of PF-03463275 (60 mg twice a day) on occupancy of GlyT1 and long-term potentiation were examined only in SZs (substudy 2). PF-03463275 at 10, 20, 40, and 60 mg twice a day produced ∼44%, 61%, 76%, and 83% GlyT1 occupancy, respectively, in SZs with higher ligand binding to GlyT1 in subcortical versus cortical regions. PF-03463275 did not attenuate any ketamine-induced effects but did improve working memory accuracy in healthy control subjects. PF-03463275 increased long-term potentiation only in SZs with peak effects at 40 mg twice a day (∼75% GlyT1 occupancy) and with a profile suggestive of an inverted U dose response. PF-03463275 was well-tolerated. The dose-related GlyT1 occupancy of PF-03463275 is linear. While PF-03463275 did not show evidence of facilitating N-methyl-D-aspartate receptor function in the ketamine assay, it enhanced neuroplasticity in SZs. These findings provide support for a clinical trial to test the ability of PF-03463275 to enhance cognitive remediation toward addressing cognitive impairments associated with schizophrenia. Published by Elsevier Inc.

  11. 27 CFR 70.97 - Failure to pay tax.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Failure to pay tax. 70.97... § 70.97 Failure to pay tax. (a) Negligence—(1) General. If any part of any underpayment (as defined in... section 6651 of the Internal Revenue Code (relating to failure to file such return or pay tax) shall be...

  12. PF-03882845, a non-steroidal mineralocorticoid receptor antagonist, prevents renal injury with reduced risk of hyperkalemia in an animal model of nephropathy

    Directory of Open Access Journals (Sweden)

    Stephen eOrena

    2013-10-01

    Full Text Available The mineralocorticoid receptor (MR antagonists PF 03882845 and eplerenone were evaluated for renal protection against aldosterone mediated renal disease in uninephrectomized Sprague Dawley (SD rats maintained on a high salt diet and receiving aldosterone by osmotic mini pump for 27 days. Serum K+ and the urinary albumin to creatinine ratio (UACR were assessed following 14 and 27 days of treatment. Aldosterone induced renal fibrosis as evidenced by increases in UACR, collagen IV staining in kidney cortex, and expression of pro fibrotic genes relative to sham operated controls not receiving aldosterone. While both PF 03882845 and eplerenone elevated serum K+ levels with similar potencies, PF 03882845 was more potent than eplerenone in suppressing the rise in UACR. PF 03882845 prevented the increase in collagen IV staining at 5, 15 and 50 mg/kg BID while eplerenone was effective only at the highest dose tested (450 mg/kg BID. All doses of PF 03882845 suppressed aldosterone induced increases in collagen IV, transforming growth factor 1 (Tgf 1, interleukin 6 (Il-6, intermolecular adhesion molecule 1 (Icam-1 and osteopontin gene expression in kidney while eplerenone was only effective at the highest dose. The therapeutic index (TI, calculated as the ratio of the EC50 for increasing serum K+ to the EC50 for UACR lowering, was 83.8 for PF 03882845 and 1.47 for eplerenone. Thus the TI of PF 03882845 against hyperkalemia was 57 fold superior to that of eplerenone indicating that PF 03882845 may present significantly less risk for hyperkalemia compared to eplerenone.

  13. Attenuation of ketamine-induced impairment in verbal learning and memory in healthy volunteers by the AMPA receptor potentiator PF-04958242.

    Science.gov (United States)

    Ranganathan, M; DeMartinis, N; Huguenel, B; Gaudreault, F; Bednar, M M; Shaffer, C L; Gupta, S; Cahill, J; Sherif, M A; Mancuso, J; Zumpano, L; D'Souza, D C

    2017-11-01

    There is a need to develop treatments for cognitive impairment associated with schizophrenia (CIAS). The significant role played by N-methyl-d-aspartate receptors (NMDARs) in both the pathophysiology of schizophrenia and in neuronal plasticity suggests that facilitation of NMDAR function might ameliorate CIAS. One strategy to correct NMDAR hypofunction is to stimulate α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) as AMPAR and NMDAR functioning are coupled and interdependent. In rats and nonhuman primates (NHP), AMPAR potentiators reduce spatial working memory deficits caused by the nonselective NMDAR antagonist ketamine. The current study assessed whether the AMPAR potentiator PF-04958242 would attenuate ketamine-induced deficits in verbal learning and memory in humans. Healthy male subjects (n=29) participated in two randomized treatment periods of daily placebo or PF-04958242 for 5 days separated by a washout period. On day 5 of each treatment period, subjects underwent a ketamine infusion for 75 min during which the effects of PF-04958242/placebo were assessed on ketamine-induced: (1) impairments in verbal learning and recall measured by the Hopkins Verbal Learning Test; (2) impairments in working memory on a CogState battery; and (3) psychotomimetic effects measured by the Positive and Negative Syndrome Scale and Clinician-Administered Dissociative Symptoms Scale. PF-04958242 significantly reduced ketamine-induced impairments in immediate recall and the 2-Back and spatial working memory tasks (CogState Battery), without significantly attenuating ketamine-induced psychotomimetic effects. There were no pharmacokinetic interactions between PF-04958242 and ketamine. Furthermore, PF-04958242 was well tolerated. 'High-impact' AMPAR potentiators like PF-04958242 may have a role in the treatment of the cognitive symptoms, but not the positive or negative symptoms, associated with schizophrenia. The excellent concordance between the

  14. PF1163A and B, new antifungal antibiotics produced by Penicillium sp. I. Taxonomy of producing strain, fermentation, isolation and biological activities.

    Science.gov (United States)

    Nose, H; Seki, A; Yaguchi, T; Hosoya, A; Sasaki, T; Hoshiko, S; Shomura, T

    2000-01-01

    Two novel antifungal antibiotics, PF1163A and B, were isolated from the fermentation broth of Penicillium sp. They were purified from the solid cultures of rice media using ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatographies. PF1163A and B showed potent growth inhibitory activity against pathogenic fungal strain Candida albicans but did not show cytotoxic activity against mammalian cells. These compounds inhibited the ergosterol biosynthesis in Candida albicans.

  15. A specific PfEMP1 is expressed in P. falciparum sporozoites and plays a role in hepatocyte infection

    DEFF Research Database (Denmark)

    Zanghì, Gigliola; Vembar, Shruthi S; Baumgarten, Sebastian

    2018-01-01

    Heterochromatin plays a central role in the process of immune evasion, pathogenesis, and transmission of the malaria parasite Plasmodium falciparum during blood stage infection. Here, we use ChIP sequencing to demonstrate that sporozoites from mosquito salivary glands expand heterochromatin......_SpzPfEMP1. Overall, we show that the epigenetic signature of var genes is reset in mosquito stages. Moreover, the identification of a strain-specific sporozoite PfEMP1 is highly relevant for vaccine design based on sporozoites....

  16. Managing Feelings about Heart Failure

    Science.gov (United States)

    ... About Heart Failure Module 6: Managing Feelings About Heart Failure Download Module Order Hardcopy Heart failure can cause ... professional help for emotional problems. Common Feelings About Heart Failure It is common for people to feel depressed ...

  17. SAM domain-dependent activity of PfTKL3, an essential tyrosine kinase-like kinase of the human malaria parasite Plasmodium falciparum.

    Science.gov (United States)

    Abdi, Abdirahman; Eschenlauer, Sylvain; Reininger, Luc; Doerig, Christian

    2010-10-01

    Over the last decade, several protein kinases inhibitors have reached the market for cancer chemotherapy. The kinomes of pathogens represent potentially attractive targets in infectious diseases. The functions of the majority of protein kinases of Plasmodium falciparum, the parasitic protist responsible for the most virulent form of human malaria, remain unknown. Here we present a thorough characterisation of PfTKL3 (PF13_0258), an enzyme that belongs to the tyrosine kinase-like kinase (TKL) group. We demonstrate by reverse genetics that PfTKL3 is essential for asexual parasite proliferation in human erythrocytes. PfTKL3 is expressed in both asexual and gametocytes stages, and in the latter the protein co-localises with cytoskeleton microtubules. Recombinant PfTKL3 displays in vitro autophosphorylation activity and is able to phosphorylate exogenous substrates, and both activities are dramatically dependent on the presence of an N-terminal "sterile alpha-motif" domain. This study identifies PfTKL3 as a validated drug target amenable to high-throughput screening.

  18. Mutations in the P-Type Cation-Transporter ATPase 4, PfATP4, Mediate Resistance to Both Aminopyrazole and Spiroindolone Antimalarials

    Science.gov (United States)

    2015-01-01

    Aminopyrazoles are a new class of antimalarial compounds identified in a cellular antiparasitic screen with potent activity against Plasmodium falciparum asexual and sexual stage parasites. To investigate their unknown mechanism of action and thus identify their target, we cultured parasites in the presence of a representative member of the aminopyrazole series, GNF-Pf4492, to select for resistance. Whole genome sequencing of three resistant lines showed that each had acquired independent mutations in a P-type cation-transporter ATPase, PfATP4 (PF3D7_1211900), a protein implicated as the novel Plasmodium spp. target of another, structurally unrelated, class of antimalarials called the spiroindolones and characterized as an important sodium transporter of the cell. Similarly to the spiroindolones, GNF-Pf4492 blocks parasite transmission to mosquitoes and disrupts intracellular sodium homeostasis. Our data demonstrate that PfATP4 plays a critical role in cellular processes, can be inhibited by two distinct antimalarial pharmacophores, and supports the recent observations that PfATP4 is a critical antimalarial target. PMID:25322084

  19. Identification of Selective Inhibitors of the Plasmodium falciparum Hexose Transporter PfHT by Screening Focused Libraries of Anti-Malarial Compounds.

    Directory of Open Access Journals (Sweden)

    Diana Ortiz

    Full Text Available Development of resistance against current antimalarial drugs necessitates the search for novel drugs that interact with different targets and have distinct mechanisms of action. Malaria parasites depend upon high levels of glucose uptake followed by inefficient metabolic utilization via the glycolytic pathway, and the Plasmodium falciparum hexose transporter PfHT, which mediates uptake of glucose, has thus been recognized as a promising drug target. This transporter is highly divergent from mammalian hexose transporters, and it appears to be a permease that is essential for parasite viability in intra-erythrocytic, mosquito, and liver stages of the parasite life cycle. An assay was developed that is appropriate for high throughput screening against PfHT based upon heterologous expression of PfHT in Leishmania mexicana parasites that are null mutants for their endogenous hexose transporters. Screening of two focused libraries of antimalarial compounds identified two such compounds that are high potency selective inhibitors of PfHT compared to human GLUT1. Additionally, 7 other compounds were identified that are lower potency and lower specificity PfHT inhibitors but might nonetheless serve as starting points for identification of analogs with more selective properties. These results further support the potential of PfHT as a novel drug target.

  20. A cross-species translational pharmacokinetic-pharmacodynamic evaluation of core body temperature reduction by the TRPM8 blocker PF-05105679.

    Science.gov (United States)

    Gosset, James R; Beaumont, Kevin; Matsuura, Tomomi; Winchester, Wendy; Attkins, Neil; Glatt, Sophie; Lightbown, Ian; Ulrich, Kristina; Roberts, Sonia; Harris, Jolie; Mesic, Emir; van Steeg, Tamara; Hijdra, Diana; van der Graaf, Piet H

    2017-11-15

    PF-05105679 is a moderately potent TRPM8 blocker which has been evaluated for the treatment of cold pain sensitivity. The TRPM8 channel is responsible for the sensation of cold environmental temperatures and has been implicated in regulation of core body temperature. Consequently, blockade of TRPM8 has been suggested to result in lowering of core body temperature. As part of the progression to human studies, the effect of PF-05105679 on core body temperature has been investigated in animals. Safety pharmacology studies showed that PF-05105679 reduced core body temperature in a manner that was inversely related to body weight of the species tested (greater exposure to PF-05105679 was required to lower temperature by 1°C in higher species). Based on an allometric (body weight) relationship, it was hypothesized that PF-05105679 would not lower core body temperature in humans at exposures that could exhibit pharmacological effects on cold pain sensation. On administration to humans, PF-05105679 was indeed effective at reversing the cold pain sensation associated with the cold pressor test in the absence of effects on core body temperature. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Normalizing the maximum permissible seal failure of the fuel cladding of VVER and the activity of the fission products in the coolant

    International Nuclear Information System (INIS)

    Luzanova, L.M.; Miglo, V.N.; Slavyagin, P.D.

    1993-01-01

    In most countries developing a nuclear power industry based on pressurized water reactors, one of the conditions for issuing a license under normal operating conditions for issuing a license stipulates that the fuel elements may not lose their hermetic seal either under normal operating conditions or during presumable disturbances of the conditions of normal use. At a conference on radiation safety the ALARA principle was taken to be fundamental, it being attempted to keep the activity of the coolant of the primary circuit, including the fission products emerging from unsealed fuel elements, to a level as low as reasonably possible. As many years of experience in the nuclear power industry have shown, nuclear power stations are in many cases operated with nonhermetic fuel elements in the core. Therefore, from the point of view of safety and economy, the best way to operate a power plant is to try to ensure maximum burnup of the fuel of the unsealed elements as they operate within the limits of safe activity of the fission products in the fuel circuits

  2. Transient phenomena in the final implosion stage at the PF-1000 facility

    International Nuclear Information System (INIS)

    Gribkov, V.A.; Banaszak, A.; Jakubowski, L.; Sadowski, M.J.; Szydlowski, A.; Bienkowska, B.; Borowiecki, M.; Ivanova-Stanik, I.; Karpinski, L.; Miklaszewski, R.A.; Paduch, M.; Scholz, M.; Tomaszewski, K.; Dubrovsky, A.V.

    2006-12-01

    This paper consists of two parts, which are essentially constituted as a single whole. Dense Plasma Focus device (DPF), which is known from the early 50's, is the oldest machine which has survived since the very beginning of thermonuclear researches. This is a high voltage high-current pulsed discharge, which is produced usually by a discharge of a capacitor bank in various gases. It consists of two coaxial electrodes with the central one (anode) spanned by a ring insulator. The device is known in two configurations of electrodes named by their creators - the Filippov type (in year 1954) and the Mather one (1961). The first configuration, elaborated in the Kurchatov Institute for Atomic Energy, Moscow, Russia, has a flat anode of disc shape lying directly on the upper edge of an insulator and a cathode ('liner') looks like a pan turned up-side down and covers the anode from the top. This is a relatively infrequent modification used at the present time in two or three centres. The second one was elaborated in the Los Alamos scientific laboratory, USA, and this has an anode in the shape of a tube with a flat lid on the top of it. Cathode is usually made in the form of a squirrel cage, i.e. containing a number of rods placed along the circumference around the anode tube. These devices on the low level of the energy stored in the bank are important due to their numerous applications. The upper level of the bank energy reached at present is interesting because of its perspectives as a unique neutron source for use in nuclear physics and radiation material sciences. The main processes taking place during a development of the discharge is described in this paper. Here we shall mention that the paper is devoted to investigations of physical processes taking place in the PF-1000 facility. This device was put into operation with full energy stored in the bank at the Institute of Plasma Physics and Laser Microfusion, Warsaw, Poland, about two years ago. The energy in the bank

  3. Automated multiple failure FMEA

    International Nuclear Information System (INIS)

    Price, C.J.; Taylor, N.S.

    2002-01-01

    Failure mode and effects analysis (FMEA) is typically performed by a team of engineers working together. In general, they will only consider single point failures in a system. Consideration of all possible combinations of failures is impractical for all but the simplest example systems. Even if the task of producing the FMEA report for the full multiple failure scenario were automated, it would still be impractical for the engineers to read, understand and act on all of the results. This paper shows how approximate failure rates for components can be used to select the most likely combinations of failures for automated investigation using simulation. The important information can be automatically identified from the resulting report, making it practical for engineers to study and act on the results. The strategy described in the paper has been applied to a range of electrical subsystems, and the results have confirmed that the strategy described here works well for realistically complex systems

  4. Heart Failure in Women

    Science.gov (United States)

    Bozkurt, Biykem; Khalaf, Shaden

    2017-01-01

    Heart failure is an important cause of morbidity and mortality in women, and they tend to develop it at an older age compared to men. Heart failure with preserved ejection fraction is more common in women than in men and accounts for at least half the cases of heart failure in women. When comparing men and women who have heart failure and a low left ventricular ejection fraction, the women are more symptomatic and have a similarly poor outcome. Overall recommendations for guideline-directed medical therapies show no differences in treatment approaches between men and women. Overall, women are generally underrepresented in clinical trials for heart failure. Further studies are needed to shed light into different mechanisms, causes, and targeted therapies of heart failure in women. PMID:29744014

  5. Definition of containment failure

    International Nuclear Information System (INIS)

    Cybulskis, P.

    1982-01-01

    Core meltdown accidents of the types considered in probabilistic risk assessments (PRA's) have been predicted to lead to pressures that will challenge the integrity of containment structures. Review of a number of PRA's indicates considerable variation in the predicted probability of containment failure as a function of pressure. Since the results of PRA's are sensitive to the prediction of the occurrence and the timing of containment failure, better understanding of realistic containment capabilities and a more consistent approach to the definition of containment failure pressures are required. Additionally, since the size and location of the failure can also significantly influence the prediction of reactor accident risk, further understanding of likely failure modes is required. The thresholds and modes of containment failure may not be independent

  6. Capturing the effect of [PF3(C2F5)3]-vs. [PF6]-, flexible anion vs. rigid, and scaled charge vs. unit on the transport properties of [bmim]+-based ionic liquids: a comparative MD study.

    Science.gov (United States)

    Kowsari, Mohammad H; Ebrahimi, Soraya

    2018-05-16

    Comprehensive molecular dynamics simulations are performed to study the average single-particle dynamics and the transport properties of 1-butyl-3-methylimidazolium hexafluorophosphate, [bmim][PF6], and 1-butyl-3-methylimidazolium tris(pentafluoroethyl)trifluorophosphate, [bmim][FAP], ionic liquids (ILs) at 400 K. We applied one of the most widely used nonpolarizable all-atom force fields for ILs, both with the original unit (±1) charges on each ion and with the partial charges uniformly scaled to 80-85%, taking into account the average polarizability and tracing the experimentally compatible transport properties. In all simulations, [bmim]+ was considered to be flexible, while the effect of a flexible vs. rigid structure of the anions and the effect of two applied charge sets on the calculated properties were separately investigated in detail. The simulation results showed that replacing [PF6]- with [FAP]-, considering anion flexibility, and applying the charge-scaled model significantly enhanced the ionic self-diffusion, ionic conductivity, inverse viscosity, and hyper anion preference (HAP). Both of the calculated self-diffusion coefficients from the long-time linear slope of the mean-square displacement (MSD) and from the integration of the velocity autocorrelation function (VACF) for the centers of mass of the ions were used for evaluation of the ionic transference number, HAP, ideal Nernst-Einstein ionic conductivity (σNE), and the Stokes-Einstein viscosity. In addition, for quantification of the degree of complicated ionic association (known as the Nernst-Einstein deviation parameter, Δ) and ionicity phenomena in the two studied ILs, the ionic conductivity was determined more rigorously by the Green-Kubo integral of the electric-current autocorrelation function (ECACF), and then the σGK/σNE ratio was evaluated. It was found that the correlated motion of the (cationanion) neighbors in [bmim][FAP] is smaller than in [bmim][PF6]. The relaxation times of

  7. The structure of the first representative of Pfam family PF09836 reveals a two-domain organization and suggests involvement in transcriptional regulation

    International Nuclear Information System (INIS)

    Das, Debanu; Grishin, Nick V.; Kumar, Abhinav; Carlton, Dennis; Bakolitsa, Constantina; Miller, Mitchell D.; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Burra, Prasad; Chen, Connie; Chiu, Hsiu-Ju; Chiu, Michelle; Clayton, Thomas; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Ernst, Dustin; Farr, Carol L.; Feuerhelm, Julie; Grzechnik, Anna; Grzechnik, Slawomir K.; Grant, Joanna C.; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Johnson, Hope A.; Klock, Heath E.; Knuth, Mark W.; Kozbial, Piotr; Krishna, S. Sri; Marciano, David; McMullan, Daniel; Morse, Andrew T.; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Oommachen, Silvya; Paulsen, Jessica; Puckett, Christina; Reyes, Ron; Rife, Christopher L.; Sefcovic, Natasha; Tien, Henry J.; Trame, Christine B.; Bedem, Henry van den; Weekes, Dana; Wooten, Tiffany; Xu, Qingping; Hodgson, Keith O.; Wooley, John; Elsliger, Marc-André; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.

    2009-01-01

    The crystal structure of the NGO1945 gene product from N. gonorrhoeae (UniProt Q5F5IO) reveals that the N-terminal domain assigned as a domain of unknown function (DUF2063) is likely to bind DNA and that the protein may be involved in transcriptional regulation. Proteins with the DUF2063 domain constitute a new Pfam family, PF09836. The crystal structure of a member of this family, NGO1945 from Neisseria gonorrhoeae, has been determined and reveals that the N-terminal DUF2063 domain is likely to be a DNA-binding domain. In conjunction with the rest of the protein, NGO1945 is likely to be involved in transcriptional regulation, which is consistent with genomic neighborhood analysis. Of the 216 currently known proteins that contain a DUF2063 domain, the most significant sequence homologs of NGO1945 (∼40–99% sequence identity) are from various Neisseria and Haemophilus species. As these are important human pathogens, NGO1945 represents an interesting candidate for further exploration via biochemical studies and possible therapeutic intervention

  8. Effect of some geometric parameters on performance of PF{sup 2} heat exchangers in periodic frosting

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Ping [Zhejiang Vocational College of Commerce, Hangzhou, Binwen Road 470 (China); Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, IL 61801 (United States); Hrnjak, P.S. [Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, IL 61801 (United States)

    2010-03-15

    The thermal performance under conditions of initial frost growth and subsequent refrosting after a defrost is experimentally studied for the parallel flow parallel fin (PF{sup 2}) heat exchanger, a new style of heat exchanger that uses louvered bent fins on flat tubes to enhance water drainage when the flat tubes are horizontal (typically outdoor heat exchanger in heat pump operation). This paper focuses on quantification of the effects of geometry (i.e. fin pitch 12-22 fpi and louver pitch 1.4-2.8 mm) on defrost and refrost times. Eight heat exchangers differing in louver pitch and fin spacing are studied. A series of tests are conducted in search for the best geometry. The effects of geometry on heat transfer (thermal performance) and pressure drop for air face velocities of 0.9, 2, and 3 m/s are determined and used for comparison. Characteristics of initial heat transfer coefficient and pressure drop during the first frosting cycle are reported in terms of Colburn j{sub 0} factor and Fanning friction f{sub 0} factor, as a function of Re{sub L{sub p}} and geometry. The newly developed air-side correlations for PF{sup 2} heat exchangers (Colburn j{sub 0} factor and Fanning friction f{sub 0} factor) predict the test data within rms error of {+-}10%, and with mean deviation of 2.95% and 4.98%, respectively. The correlations are base on a very low Reynolds numbers in the range of 100-620, and 8 PF{sup 2} heat exchangers using 48 experimental data. (author)

  9. Productivity

    DEFF Research Database (Denmark)

    Spring, Martin; Johnes, Geraint; Hald, Kim Sundtoft

    Productivity is increasingly critical for developed economies. It has always been important: as Paul Krugman puts it, “Productivity isn’t everything, but in the long run it is almost everything. A country’s ability to improve its standard of living over time depends almost entirely on its ability...... to raise its output per worker”(Krugman, 1994). Analyses of productivity have, by and large, been the preserve of economists. Operations Management (OM) is rooted in a similar concern for the efficient use of scarce resources; Management Accounting (MA) is concerned with the institutionalised measurement...... and management of productivity. Yet the three perspectives are rarely connected. This paper is a sketch of a literature review seeking to identify, contrast and reconcile these three perspectives. In so doing, it aims to strengthen the connections between policy and managerial analyses of productivity....

  10. A Direct Quantitative Agar-Plate Based Assay for Analysis of Pseudomonas protegens PF-5 Degradation of Polyurethane Films (Postprint)

    Science.gov (United States)

    2014-10-02

    was dissolved in methanol ; Irogran (1 g) was dissolved in 25 mL THF; and AS-P108 was prepared by adding 0.3 g of catalyst to 10 g of resin and then... Selected results are in Supple- mental Information. 3. Results 3.1. General growth characteristics of Pf-5 on M9/citrate agar plates Dilutions of P...minimal. An IR map with the color scale corresponding to the in- tensity of the 1735 cm1 carbonyl absorbance from the urethane and ester components is

  11. AP600 large-break loss-of-collant-accident developmental assessment plan for TRAC-PF1/MOD2

    International Nuclear Information System (INIS)

    Knight, T.D.

    1996-07-01

    The Westinghouse AP600 reactor is an advanced pressurized water reactor with passive safety systems to protect the plant against possible accidents and transients. The design has been submitted to the U.S. NRC for design certification. The NRC has selected the Transient Reactor Analysis Code (TRAC)-PF1/MOD2 for performing large break loss-of coolant-accident (LBLOCA) analysis to support the certification effort. This document defines the tests to be used in the current phase of developmental assessment related to AP600 LBLOCA

  12. TRAC-PF1 analysis of LOFT steam-generator feedwater transient test L9-1

    International Nuclear Information System (INIS)

    Meier, J.K.

    1983-01-01

    The Transient Reactor Analysis Code (TRAC-PF1) calculations were compared to test data from Loss-of-Fluid Test (LOFT) L9-1, which was a loss-of-feedwater transient. This paper includes descriptions of the test and the TRAC input and compares the TRAC-calculated results with the test data. We conclude that the code predicted the experiment well, given the uncertainties in the boundary conditions. The analysis indicates the need to model all the flow paths and heat structures, and to improve the TRAC wall condensation heat-transfer model

  13. Mass- and energy-analysis of fast ion beams in PF-1000 by means of a Thomson spectrometer

    Energy Technology Data Exchange (ETDEWEB)

    Czaus, K.; Skladnik-Sadowska, E.; Malinowski, K.; Kwiatkowski, R.; Zebrowski, J. [The Andrzej Soltan Institute for Nuclear Studies - IPJ, 05-400 Otwock-Swierk (Poland); Sadowski, M.J. [The Andrzej Soltan Institute for Nuclear Studies - IPJ, 05-400 Otwock-Swierk (Poland)] [Institute of Plasma Physics and Laser Microfusion - IPPLM, 01-497 Warsaw (Poland); Zebrowski, J.; Karpinski, L.; Paduch, M.; Scholz, M.; Zielinska, E. [Institute of Plasma Physics and Laser Microfusion - IPPLM, 01-497 Warsaw (Poland); Garkusha, I.E. [Institute of Plasma Physics, NSC KIPT, 61-108 Kharkov (Ukraine)

    2011-07-01

    The paper describes measurements of energy spectra of ions emitted along the z-axis of the PF-1000 facility, which have been for the first time performed by means a miniature Thomson spectrometer during 480-kJ discharges with the deuterium filling. The recorded Thomson parabolas showed that the escaping deuterons have energies in the range of 25-1000 keV, while protons (originated from hydrogen remnants) have the population of about 2 orders smaller and energies within the range of 35-300 keV. This document is composed of a paper followed by a poster. (authors)

  14. PfEMP1 – A Parasite Protein Family of Key Importance in Plasmodium falciparum Malaria Immunity and Pathogenesis

    DEFF Research Database (Denmark)

    Hviid, Lars; Jensen, Anja T R

    2015-01-01

    to be a central element in the pathogenesis of the disease. It is mediated by the interaction of parasite ligands on the erythrocyte surface and a range of host receptor molecules in many organs and tissues. Among several proteins and protein families implicated in this process, the P. falciparum erythrocyte...... membrane protein 1 (PfEMP1) family of high-molecular weight and highly variable antigens appears to be the most prominent. In this chapter, we aim to provide a systematic overview of the current knowledge about these proteins, their structure, their function, how they are presented on the erythrocyte...

  15. Common cause failures - a dilemma in perspective

    International Nuclear Information System (INIS)

    Smith, A.M.; Watson, I.A.

    1980-01-01

    This paper identifies the broad spectrum of common cause failure (CCF) definitions used by various authors. These definitions, as applied to real aircraft and nuclear reactor failure events, lead to a divergence of interpretation and a resultant confusion that obscures meaningful progress in CCF analysis. A new definition is proposed, explained, and tested against the examples. Technical as well as administrative practices are cited as ways to control or eliminate the product defects that lead to CCF. (author)

  16. Immune mediated liver failure.

    Science.gov (United States)

    Wang, Xiaojing; Ning, Qin

    2014-01-01

    Liver failure is a clinical syndrome of various etiologies, manifesting as jaundice, encephalopathy, coagulopathy and circulatory dysfunction, which result in subsequent multiorgan failure. Clinically, liver failure is classified into four categories: acute, subacute, acute-on-chronic and chronic liver failure. Massive hepatocyte death is considered to be the core event in the development of liver failure, which occurs when the extent of hepatocyte death is beyond the liver regenerative capacity. Direct damage and immune-mediated liver injury are two major factors involved in this process. Increasing evidence has suggested the essential role of immune-mediated liver injury in the pathogenesis of liver failure. Here, we review the evolved concepts concerning the mechanisms of immune-mediated liver injury in liver failure from human and animal studies. Both innate and adaptive immunity, especially the interaction of various immune cells and molecules as well as death receptor signaling system are discussed. In addition, we highlight the concept of "immune coagulation", which has been shown to be related to the disease progression and liver injury exacerbation in HBV related acute-on-chronic liver failure.

  17. The Reconstruction of Failure

    Science.gov (United States)

    Turner, Ralph H.

    1972-01-01

    Seeks to explain the use of ideologies concerning the failure or non-failure in American education in terms of the struggle of sponsoring and professional groups to establish or maintain position on the social scene and, correspondingly, to gain control over those clienteles which are relevant to drives for social or professional mobility. (RJ)

  18. Success by Failure

    Science.gov (United States)

    Andrews, Benjamin D.

    2017-01-01

    By taking the step to talk openly about his failure, the author stumbled upon one of several important lessons that he learned from this experience. The author recognized that he did not have to pretend anymore. In this article, the author shares his story of developing a "growth mindset" to overcome his own failure and support his…

  19. Central oxygen pipeline failure

    African Journals Online (AJOL)

    surgical intensive care unit (ICU), with two patients on full ventilation and ... uncertainty around the cause of the failure and the restoration, .... soon as its level also falls below three tons. Should ... (properly checked and closed prior to each anaesthetic). ... in use at the time of the central oxygen pipeline failure at Tygerberg.

  20. Radiology of renal failure

    International Nuclear Information System (INIS)

    Griffiths, H.J.

    1990-01-01

    This book covers most aspects of imaging studies in patients with renal failure. The initial chapter provides basic information on contrast agents, intravenous urography, and imaging findings in the urinary tract disorders responsible for renal failure and in patients who have undergone transplantation. It illustrates common gastro-intestinal abnormalities seen on barium studies in patients with renal failure. It illustrates the cardiopulmonary complications of renal failure and offers advice for radiologic differentiation. It details different aspects of skeletal changes in renal failure, including a basic description of the pathophysiology of the changes; many excellent illustrations of classic bone changes, arthritis, avascular necrosis, and soft-tissue calcifications; and details of bone mineral analysis

  1. SOR-ring failure

    International Nuclear Information System (INIS)

    Kitamura, Hideo

    1981-01-01

    It was in the autumn of 1976 that the SOR-ring (synchrotron radiation storage ring) has commenced the regular operation. Since then, the period when the operation was interrupted due to the failures of SOR-ring itself is in total about 8 weeks. Failures and accidents have occurred most in the vacuum system. Those failure experiences are described on the vacuum, electromagnet, radio-frequency acceleration and beam transport systems with their interrupted periods. The eleven failures in the vacuum system have been reported, such as bellows breakage in a heating-evacuating period, leakage from the bellows of straight-through valves (made in U.S.A. and Japan), and leakage from the joint flange of the vacuum system. The longest interruption was 5 weeks due to the failure of a domestically manufactured straight-through valve. The failures of the electromagnet system involve the breakage in a cooling water system, short circuit of a winding in the Q magnet power transformer, blow of a fuse protecting the deflection magnet power source by the current less than the rating, and others. The failures of the RF acceleration system include the breakage of an output electronic tube the breakage of a cavity ceramic, RF voltage fluctuation due to the contact deterioration at a cavity electrode, and the failure of grid bias power source. It is necessary to select the highly reliable components for the vacuum system because the vacuum system failures require longer time for recovery, and very likely to induce secondary and tertiary failures. (Wakatsuki, Y.)

  2. When Middle Really Means "Top" or "Bottom": An Analysis of the 16PF5 Using Bock's Nominal Response Model.

    Science.gov (United States)

    Murray, Aja Louise; Booth, Tom; Molenaar, Dylan

    2016-01-01

    When self-report items with a Likert-type scale include a middle response option (e.g., Unsure, Neither agree nor disagree, or ?), this middle option is assumed to measure a level of the trait intermediate between the high and low response categories. In this study, we tested this assumption in the 16 Personality Factor Questionnaire, Version 5 (16PF5) by fitting Bock's nominal response model in the U.S. and UK standardization samples of the 16PF5. We found that in many cases, the middle option was indicative of higher levels of the latent trait than the ostensibly highest response option. In certain other cases, it was indicative of lower levels of the latent trait than the ostensibly lowest response option. This undermines the use of a simple successive integer scoring scheme where responses in adjacent response categories are assigned scores of 0, 1, and 2. Recommendations for alternative scoring schemes are provided. Results also suggested that certain personality traits, especially neurotic traits, are associated with a tendency toward selecting the middle option.

  3. Numerical analysis on ingress-of-coolant events in fusion reactors with TRAC-PF1 code

    International Nuclear Information System (INIS)

    Ose, Yasuo; Takase, Kazuyuki; Akimoto, Hajime

    2000-01-01

    As for accident events related with thermal-hydraulics, in a fusion experimental reactor an ingress-of-coolant event (ICE) and a loss-of-vacuum-accident event (LOVA) should be considered. An integrated ICE/LOVA test apparatus is under planning in order to estimate quantitatively heat transfer and fluid flow characteristics under ICE and LOVA events. This study was carried out to predict numerically the thermal-hydraulic characteristics in fusion reactors at the ICE events before construction of the integrated ICE/LOVA test apparatus. The TRAC-PF1 code, which was originally developed for the thermal-hydraulic safety analysis in light water reactors, was used. The numerical analyses were performed for two kinds of system configuration with/without a pressure-suppression tank:the former for is investigation of the pressure rise characteristics and two-phase flow behavior; the latter for estimation of an effect of the pressure reduction due to the pressure-suppression tank. From the present analytical results, effects of the ingress water flow rate and vessel temperatures on the pressure rise ware clarified quantitatively. Furthermore, the pressure-rise suppression effect due to the vapor condensation in the pressure-suppression tank was predicted numerically. In addition, the useful information regarding to the design of the integrated ICE/LOVA test apparatus and the knowledge with respect to the effective usage of the TRAC-PF1 code were obtained through the present numerical study. (author)

  4. Ex post and ex ante willingness to pay (WTP) for the ICT Malaria Pf/Pv test kit in Myanmar.

    Science.gov (United States)

    Cho-Min-Naing; Lertmaharit, S; Kamol-Ratanakul, P; Saul, A J

    2000-03-01

    Willingness to pay (WTP) for the ICT Malaria Pf/Pv test kit was assessed by the contingent valuation method using a bidding game approach in two villages in Myanmar. Kankone (KK) village has a rural health center (RHC) and Yae-Aye-Sann (YAS) is serviced by community health worker (CHW). The objectives were to assess WTP for the ICT Malaria Pf/Pv test kit and to determine factors affecting the WTP. In both villages WTP was assessed in two different conditions, ex post and ex ante. The ex post WTP was assessed at an RHC in the KK village and at the residence of a CHW in the YAS village on patients immediately following diagnosis of malaria. The ex ante WTP was assessed by household interviews in both villages on people with a prior history of malaria. Ordinary least squares (OLS) multiple regression analysis was used to analyze factors affecting WTP. The WTP was higher in ex post conditions than ex ante in both villages. WTP was significantly positively associated with the average monthly income of the respondents and severity of illness in both ex post and ex ante conditions (p WTP (p < 0.05) in the ex post condition in the RHC survey in KK village.

  5. [BMIM][PF6] Incorporation Doubles CO2 Selectivity of ZIF-8: Elucidation of Interactions and Their Consequences on Performance.

    Science.gov (United States)

    Kinik, F Pelin; Altintas, Cigdem; Balci, Volkan; Koyuturk, Burak; Uzun, Alper; Keskin, Seda

    2016-11-16

    Experiments were combined with atomically detailed simulations and density functional theory (DFT) calculations to understand the effect of incorporation of an ionic liquid (IL), 1-n-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF 6 ]), into a metal organic framework (MOF with a zeolitic imidazolate framework), ZIF-8, on the CO 2 separation performance. The interactions between [BMIM][PF 6 ] and ZIF-8 were examined in deep detail, and their consequences on CO 2 /CH 4 , CO 2 /N 2 , and CH 4 /N 2 separation have been elucidated by using experimental measurements complemented by DFT calculations and atomically detailed simulations. Results suggest that IL-MOF interactions strongly affect the gas affinity of materials at low pressure, whereas available pore volume plays a key role for gas adsorption at high pressures. Direct interactions between IL and MOF lead to at least a doubling of CO 2 /CH 4 and CO 2 /N 2 selectivities of ZIF-8. These results provide opportunities for rational design and development of IL-incorporated MOFs with exceptional selectivity for target gas separation applications.

  6. Rate theory of solvent exchange and kinetics of Li(+) - BF4 (-)/PF6 (-) ion pairs in acetonitrile.

    Science.gov (United States)

    Dang, Liem X; Chang, Tsun-Mei

    2016-09-07

    In this paper, we describe our efforts to apply rate theories in studies of solvent exchange around Li(+) and the kinetics of ion pairings in lithium-ion batteries (LIBs). We report one of the first computer simulations of the exchange dynamics around solvated Li(+) in acetonitrile (ACN), which is a common solvent used in LIBs. We also provide details of the ion-pairing kinetics of Li(+)-[BF4] and Li(+)-[PF6] in ACN. Using our polarizable force-field models and employing classical rate theories of chemical reactions, we examine the ACN exchange process between the first and second solvation shells around Li(+). We calculate exchange rates using transition state theory and weighted them with the transmission coefficients determined by the reactive flux, Impey, Madden, and McDonald approaches, and Grote-Hynes theory. We found the relaxation times changed from 180 ps to 4600 ps and from 30 ps to 280 ps for Li(+)-[BF4] and Li(+)-[PF6] ion pairs, respectively. These results confirm that the solvent response to the kinetics of ion pairing is significant. Our results also show that, in addition to affecting the free energy of solvation into ACN, the anion type also should significantly influence the kinetics of ion pairing. These results will increase our understanding of the thermodynamic and kinetic properties of LIB systems.

  7. Laser photolysis study of anthraquinone in binary mixtures ofionic liquid [bmim][PF6] and organic solvent

    Directory of Open Access Journals (Sweden)

    Side Yao

    2006-12-01

    Full Text Available Photochemical properties of the ionic liquid (RTIL 1-butyl-3-methylimidazoliumhexafluorophosphate [bmim][PF6] and its binary mixed solutions with organic solvent(DMF and MeCN were investigated by laser photolysis at an excitation wavelength of 355nm, using anthraquinone (AQ as a probe molecule. It was indicated that the triplet excitedstate of AQ (3AQ* can abstract hydrogen from [bmim][PF6]. Moreover, along with thechange of the ratio of RTIL and organic solvent, the reaction rate constant changes regularly.Critical points were observed at volume fraction VRTIL = 0.2 for RTIL/MeCN and VRTIL =0.05 for RTIL/DMF. For both systems, before the critical point, the rate constant increasesrapidly with increasing VRTIL; however, it decreases obviously with VRTIL after the criticalpoint. We conclude that the concentration dependence is dominant at lower VRTIL, while theviscosity and phase transformation are dominant at higher VRTIL for the effect of ionic liquidon the decay of rate constant.

  8. Ultra-fast and sensitive photo-induced phase switching in (EDO-TTF)2PF6

    International Nuclear Information System (INIS)

    Chollet, Matthieu; Guerin, Laurent; Uchida, Naoki; Fukaya, Souichi; Ishikawa, Tadahiko; Koshihara, Shin-ya; Matsuda, Kazunari; Yamochi, Hideki; Ota, Akira; Saito, Gunzi

    2005-01-01

    Organic conductor (EDO-TTF) 2 PF 6 crystal having 14 filled band shows a metal (M)-insulator (I) transition accompanied with Peierls transition, charge ordering, and anion ordering at transition temperature, T C =280K. This crystal is an important and fascinating candidate for photo-induced M-I transition because the multi-instability will afford sensitivity to the tiny stimulation. We make the report of the reflectivity change in (EDO-TTF) 2 PF 6 crystal induced by the irradiation of femto-second (fs) pulsed laser (pulse width: 120fs, main wavelength: 800nm, repetition rate: 1kHz). The obtained results indicate that the highly efficient I-to-M transition occurs within 3ps in this material. Based on these results, the strong electron-lattice cooperative interaction is proved to play an essential role in the driving process of this M-I transition. Also, 14 filled materials, which show M-I transition, accompanied with the charge ordering, can be classified as fascinating candidates not only for superconductivity but also for photo-induced cooperative phenomena and application in phase switching devices

  9. Uncertainties in modelling and scaling of critical flows and pump model in TRAC-PF1/MOD1

    International Nuclear Information System (INIS)

    Rohatgi, U.S.; Yu, Wen-Shi.

    1987-01-01

    The USNRC has established a Code Scalability, Applicability and Uncertainty (CSAU) evaluation methodology to quantify the uncertainty in the prediction of safety parameters by the best estimate codes. These codes can then be applied to evaluate the Emergency Core Cooling System (ECCS). The TRAC-PF1/MOD1 version was selected as the first code to undergo the CSAU analysis for LBLOCA applications. It was established through this methodology that break flow and pump models are among the top ranked models in the code affecting the peak clad temperature (PCT) prediction for LBLOCA. The break flow model bias or discrepancy and the uncertainty were determined by modelling the test section near the break for 12 Marviken tests. It was observed that the TRAC-PF1/MOD1 code consistently underpredicts the break flow rate and that the prediction improved with increasing pipe length (larger L/D). This is true for both subcooled and two-phase critical flows. A pump model was developed from Westinghouse (1/3 scale) data. The data represent the largest available test pump relevant to Westinghouse PWRs. It was then shown through the analysis of CE and CREARE pump data that larger pumps degrade less and also that pumps degrade less at higher pressures. Since the model developed here is based on the 1/3 scale pump and on low pressure data, it is conservative and will overpredict the degradation when applied to PWRs

  10. Identification and functional validation of the novel antimalarial resistance locus PF10_0355 in Plasmodium falciparum.

    Directory of Open Access Journals (Sweden)

    Daria Van Tyne

    2011-04-01

    Full Text Available The Plasmodium falciparum parasite's ability to adapt to environmental pressures, such as the human immune system and antimalarial drugs, makes malaria an enduring burden to public health. Understanding the genetic basis of these adaptations is critical to intervening successfully against malaria. To that end, we created a high-density genotyping array that assays over 17,000 single nucleotide polymorphisms (∼ 1 SNP/kb, and applied it to 57 culture-adapted parasites from three continents. We characterized genome-wide genetic diversity within and between populations and identified numerous loci with signals of natural selection, suggesting their role in recent adaptation. In addition, we performed a genome-wide association study (GWAS, searching for loci correlated with resistance to thirteen antimalarials; we detected both known and novel resistance loci, including a new halofantrine resistance locus, PF10_0355. Through functional testing we demonstrated that PF10_0355 overexpression decreases sensitivity to halofantrine, mefloquine, and lumefantrine, but not to structurally unrelated antimalarials, and that increased gene copy number mediates resistance. Our GWAS and follow-on functional validation demonstrate the potential of genome-wide studies to elucidate functionally important loci in the malaria parasite genome.

  11. Standard guide for corrosion-related failure analysis

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2000-01-01

    1.1 This guide covers key issues to be considered when examining metallic failures when corrosion is suspected as either a major or minor causative factor. 1.2 Corrosion-related failures could include one or more of the following: change in surface appearance (for example, tarnish, rust, color change), pin hole leak, catastrophic structural failure (for example, collapse, explosive rupture, implosive rupture, cracking), weld failure, loss of electrical continuity, and loss of functionality (for example, seizure, galling, spalling, swelling). 1.3 Issues covered include overall failure site conditions, operating conditions at the time of failure, history of equipment and its operation, corrosion product sampling, environmental sampling, metallurgical and electrochemical factors, morphology (mode) or failure, and by considering the preceding, deducing the cause(s) of corrosion failure. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibili...

  12. Magnet failure workshop

    Energy Technology Data Exchange (ETDEWEB)

    Marston, P G [Massachusetts Institute of Technology, Cambridge (U.S.A.); Desportes, H [C.E.N.-Saclay, 91 - Gif-sur-Yvette (France); Morpurgo, M [C.E.R.N., Geneva (Switzerland); Komarek, P [Kernforschungszentrum, Karlsruhe (Germany, FR); Van Hulst, K [University of Nijmegen (The Netherlands); Hackley, D [General Dynamics Convair, San Diego, CA (U.S.A.); Young, J L [Westinghouse Electric Corporation, East Pittsburgh, PE (U.S.A.); Kibbe, K [Oak Ridge National Laboratory (U.S.A.)

    1984-01-01

    The dictionary defines failure as nonperformance or an unacceptable want of success. (The definition implies that the results should have been avoided). In an emerging technology such as ours, the boundaries of expectations and ''unacceptable success'' are often ill defined. Failures are often touted as hugh successes (occasionally rightfully so) and exceptional technological achievement can become dismal failure (if expectations are too high). We all, however, have experienced problems of one sort or another and the basic purpose of the workshop was simply to communicate these so that new or future workers don't make the same mistakes.

  13. Failure Diameter Resolution Study

    Energy Technology Data Exchange (ETDEWEB)

    Menikoff, Ralph [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-12-19

    Previously the SURFplus reactive burn model was calibrated for the TATB based explosive PBX 9502. The calibration was based on fitting Pop plot data, the failure diameter and the limiting detonation speed, and curvature effect data for small curvature. The model failure diameter is determined utilizing 2-D simulations of an unconfined rate stick to find the minimum diameter for which a detonation wave propagates. Here we examine the effect of mesh resolution on an unconfined rate stick with a diameter (10mm) slightly greater than the measured failure diameter (8 to 9 mm).

  14. Magnet failure workshop

    International Nuclear Information System (INIS)

    Marston, P.G.; Desportes, H.; Morpurgo, M.; Komarek, P.; Van Hulst, K.; Hackley, D.; Young, J.L.; Kibbe, K.

    1984-01-01

    The dictionary defines failure as nonperformance or an unacceptable want of success. (The definition implies that the results should have been avoided). In an emerging technology such as ours, the boundaries of expectations and ''unacceptable success'' are often ill defined. Failures are often touted as hugh successes (occasionally rightfully so) and exceptional technological achievement can become dismal failure (if expectations are too high). We all, however, have experienced problems of one sort or another and the basic purpose of the workshop was simply to communicate these so that new or future workers don't make the same mistakes

  15. Nutrition in Heart Failure

    OpenAIRE

    Reci Meseri

    2013-01-01

    Heart failure is defined as decreased ability of heart due to various reasons. It%u2019s seen 2-3% but the prevalence increases sharply after the age of seventy. The objectives of nutrition therapy in heart failure are to prevent from water retention and edema, to avoid from hard digestion and to offer a balanced diet. In order to avoid fluid retention and edema, daily sodium and fluid intake must be monitored carefully. Main dilemma of the heart failure patients is the obesity-cachexia dilem...

  16. Neurological Respiratory Failure

    Directory of Open Access Journals (Sweden)

    Mohan Rudrappa

    2018-01-01

    Full Text Available West Nile virus infection in humans is mostly asymptomatic. Less than 1% of neuro-invasive cases show a fatality rate of around 10%. Acute flaccid paralysis of respiratory muscles leading to respiratory failure is the most common cause of death. Although the peripheral nervous system can be involved, isolated phrenic nerve palsy leading to respiratory failure is rare and described in only two cases in the English literature. We present another case of neurological respiratory failure due to West Nile virus-induced phrenic nerve palsy. Our case reiterates the rare, but lethal, consequences of West Nile virus infection, and the increase of its awareness among physicians.

  17. Detecting failure of climate predictions

    Science.gov (United States)

    Runge, Michael C.; Stroeve, Julienne C.; Barrett, Andrew P.; McDonald-Madden, Eve

    2016-01-01

    The practical consequences of climate change challenge society to formulate responses that are more suited to achieving long-term objectives, even if those responses have to be made in the face of uncertainty1, 2. Such a decision-analytic focus uses the products of climate science as probabilistic predictions about the effects of management policies3. Here we present methods to detect when climate predictions are failing to capture the system dynamics. For a single model, we measure goodness of fit based on the empirical distribution function, and define failure when the distribution of observed values significantly diverges from the modelled distribution. For a set of models, the same statistic can be used to provide relative weights for the individual models, and we define failure when there is no linear weighting of the ensemble models that produces a satisfactory match to the observations. Early detection of failure of a set of predictions is important for improving model predictions and the decisions based on them. We show that these methods would have detected a range shift in northern pintail 20 years before it was actually discovered, and are increasingly giving more weight to those climate models that forecast a September ice-free Arctic by 2055.

  18. Acute heart failure syndrome

    African Journals Online (AJOL)

    and the classical syndrome of chronic persistent heart failure develops. The vast ... Flash pulmonary oedema: This is a severely elevated blood pressure with an .... (CPAP or bilevelNPPV) for cardiogenic pulmonary edema (review). Cochrane.

  19. Failure to Fail

    Directory of Open Access Journals (Sweden)

    Samuel Vriezen

    2013-07-01

    Full Text Available Between pessimism and optimism, Samuel Vriezen attempts to intuit a third way through an assessment of failure and negativity in the consonances and tensions between the prosody of Irish playwright Samuel Becekett and American poet Gertrude Stein.

  20. Inverter ratio failure detector

    Science.gov (United States)

    Wagner, A. P.; Ebersole, T. J.; Andrews, R. E. (Inventor)

    1974-01-01

    A failure detector which detects the failure of a dc to ac inverter is disclosed. The inverter under failureless conditions is characterized by a known linear relationship of its input and output voltages and by a known linear relationship of its input and output currents. The detector includes circuitry which is responsive to the detector's input and output voltages and which provides a failure-indicating signal only when the monitored output voltage is less by a selected factor, than the expected output voltage for the monitored input voltage, based on the known voltages' relationship. Similarly, the detector includes circuitry which is responsive to the input and output currents and provides a failure-indicating signal only when the input current exceeds by a selected factor the expected input current for the monitored output current based on the known currents' relationship.

  1. Acute kidney failure

    Science.gov (United States)

    ... Renal failure - acute; ARF; Kidney injury - acute Images Kidney anatomy References Devarajan P. Biomarkers for assessment of renal function during acute kidney injury. In: Alpern RJ, Moe OW, Caplan M, ...

  2. Life Support with Failures and Variable Supply

    Science.gov (United States)

    Jones, Harry

    2010-01-01

    The life support system for long duration missions will recycle oxygen and water to reduce the material resupply mass from Earth. The impact of life support failures was investigated by dynamic simulation of a lunar outpost habitat life support model. The model was modified to simulate resupply delays, power failures, recycling system failures, and storage failures. Many failures impact the lunar outpost water supply directly or indirectly, depending on the water balance and water storage. Failure effects on the water supply are reduced if Extra Vehicular Activity (EVA) water use is low and the water supply is ample. Additional oxygen can be supplied by scavenging unused propellant or by production from regolith, but the amounts obtained can vary significantly. The requirements for oxygen and water can also vary significantly, especially for EVA. Providing storage buffers can improve efficiency and reliability, and minimize the chance of supply failing to meet demand. Life support failures and supply variations can be survivable if effective solutions are provided by the system design

  3. Acute heart failure

    OpenAIRE

    Sénior Sánchez, Juan Manuel; Gándara Ricardo, Jairo Alfonso

    2015-01-01

    We describe the clinical case of a 26 year-old woman who came to Hospital Universitario San Vicente Fundación (Medellín, Colombia) with symptoms and signs of acute heart failure. She had been previously diagnosed with chronic heart failure with reduced ejection fraction without clear origin, pulmonary thromboembolism and ischemic stroke, without optimal neurohormonal modulation. She was admitted with clinical findings of fluid overload and low tissue perfusion, with inotropic support requirem...

  4. Investigation of the biological properties of Cinnulin PF in the context of diabetes: mechanistic insights by genome-wide mRNA-Seq analysis.

    Science.gov (United States)

    Rafehi, Haloom; Ververis, Katherine; Balcerczyk, Aneta; Ziemann, Mark; Ooi, Jenny; Hu, Sean; Kwa, Faith A A; Loveridge, Shanon J; Georgiadis, George T; El-Osta, Assam; Karagiannis, Tom C

    2012-01-01

    The accumulating evidence of the beneficial effects of cinnamon (Cinnamomum burmanni) in type-2 diabetes, a chronic age-associated disease, has prompted the commercialisation of various supplemental forms of the spice. One such supplement, Cinnulin PF(®), represents the water soluble fraction containing relatively high levels of the double-linked procyanidin type-A polymers of flavanoids. The overall aim of this study was to utilize genome-wide mRNA-Seq analysis to characterise the changes in gene expression caused by Cinnulin PF in immortalised human keratinocytes and microvascular endothelial cells, which are relevant with respect to diabetic complications. In summary, our findings provide insights into the mechanisms of action of Cinnulin PF in diabetes and diabetic complications. More generally, we identify relevant candidate genes which could provide the basis for further investigation.

  5. α2-macroglobulin can crosslink multiple Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) molecules and may facilitate adhesion of parasitized erythrocytes

    DEFF Research Database (Denmark)

    Stevenson, Liz; Laursen, Erik; Cowan, Graeme J

    2015-01-01

    -macroglobulin (α2M), which is both required and sufficient for rosetting mediated by the PfEMP1 protein HB3VAR06 and some other rosette-mediating PfEMP1 proteins. We map the α2M binding site to the C terminal end of HB3VAR06, and demonstrate that α2M can bind at least four HB3VAR06 proteins, plausibly....... Together, our results are evidence that P. falciparum parasites exploit α2M (and IgM) to expand the repertoire of host receptors available for PfEMP1-mediated IE adhesion, such as the erythrocyte carbohydrate moieties that lead to formation of rosettes. It is likely that this mechanism also affects IE...

  6. Failure analysis on a ruptured petrochemical pipe

    Energy Technology Data Exchange (ETDEWEB)

    Harun, Mohd [Industrial Technology Division, Malaysian Nuclear Agency, Ministry of Science, Technology and Innovation Malaysia, Bangi, Kajang, Selangor (Malaysia); Shamsudin, Shaiful Rizam; Kamardin, A. [Univ. Malaysia Perlis, Jejawi, Arau (Malaysia). School of Materials Engineering

    2010-08-15

    The failure took place on a welded elbow pipe which exhibited a catastrophic transverse rupture. The failure was located on the welding HAZ region, parallel to the welding path. Branching cracks were detected at the edge of the rupture area. Deposits of corrosion products were also spotted. The optical microscope analysis showed the presence of transgranular failures which were related to the stress corrosion cracking (SCC) and were predominantly caused by the welding residual stress. The significant difference in hardness between the welded area and the pipe confirmed the findings. Moreover, the failure was also caused by the low Mo content in the stainless steel pipe which was detected by means of spark emission spectrometer. (orig.)

  7. A Failure Locus for Hydrogen Assisted Failure

    DEFF Research Database (Denmark)

    Fuentes-Alonso, Sandra; Harris, Zach D.; Burns, James T.

    2017-01-01

    of a hydrogen-dependent traction separation law. A special control algorithm is employed to overcome numerical instabilities intrinsically associated with cohesive zone formulations. The fracture energy is degraded by means of an experimentally-motivated hydrogen degradation relation. Numerical results provide...... important insight into the failure process, enabling to identify critical values of hydrogen concentration and remote stresses that trigger cracking. The work builds upon previous works by the authors and brings important insight into the technologically important problem of hydrogen assisted cracking....

  8. Artemether resistance in vitro is linked to mutations in PfATP6 that also interact with mutations in PfMDR1 in travellers returning with Plasmodium falciparum infections.

    KAUST Repository

    Pillai, Dylan R; Lau, Rachel; Khairnar, Krishna; Lepore, Rosalba; Via, Allegra; Staines, Henry M; Krishna, Sanjeev

    2012-01-01

    BACKGROUND: Monitoring resistance phenotypes for Plasmodium falciparum, using in vitro growth assays, and relating findings to parasite genotype has proved particularly challenging for the study of resistance to artemisinins. METHODS: Plasmodium falciparum isolates cultured from 28 returning travellers diagnosed with malaria were assessed for sensitivity to artemisinin, artemether, dihydroartemisinin and artesunate and findings related to mutations in pfatp6 and pfmdr1. RESULTS: Resistance to artemether in vitro was significantly associated with a pfatp6 haplotype encoding two amino acid substitutions (pfatp6 A623E and S769N; (mean IC50 (95% CI) values of 8.2 (5.7 - 10.7) for A623/S769 versus 623E/769 N 13.5 (9.8 - 17.3) nM with a mean increase of 65%; p = 0.012). Increased copy number of pfmdr1 was not itself associated with increased IC50 values for artemether, but when interactions between the pfatp6 haplotype and increased copy number of pfmdr1 were examined together, a highly significant association was noted with IC50 values for artemether (mean IC50 (95% CI) values of 8.7 (5.9 - 11.6) versus 16.3 (10.7 - 21.8) nM with a mean increase of 87%; p = 0.0068). Previously described SNPs in pfmdr1 are also associated with differences in sensitivity to some artemisinins. CONCLUSIONS: These findings were further explored in molecular modelling experiments that suggest mutations in pfatp6 are unlikely to affect differential binding of artemisinins at their proposed site, whereas there may be differences in such binding associated with mutations in pfmdr1. Implications for a hypothesis that artemisinin resistance may be exacerbated by interactions between PfATP6 and PfMDR1 and for epidemiological studies to monitor emerging resistance are discussed.

  9. Artemether resistance in vitro is linked to mutations in PfATP6 that also interact with mutations in PfMDR1 in travellers returning with Plasmodium falciparum infections.

    KAUST Repository

    Pillai, Dylan R

    2012-04-27

    BACKGROUND: Monitoring resistance phenotypes for Plasmodium falciparum, using in vitro growth assays, and relating findings to parasite genotype has proved particularly challenging for the study of resistance to artemisinins. METHODS: Plasmodium falciparum isolates cultured from 28 returning travellers diagnosed with malaria were assessed for sensitivity to artemisinin, artemether, dihydroartemisinin and artesunate and findings related to mutations in pfatp6 and pfmdr1. RESULTS: Resistance to artemether in vitro was significantly associated with a pfatp6 haplotype encoding two amino acid substitutions (pfatp6 A623E and S769N; (mean IC50 (95% CI) values of 8.2 (5.7 - 10.7) for A623/S769 versus 623E/769 N 13.5 (9.8 - 17.3) nM with a mean increase of 65%; p = 0.012). Increased copy number of pfmdr1 was not itself associated with increased IC50 values for artemether, but when interactions between the pfatp6 haplotype and increased copy number of pfmdr1 were examined together, a highly significant association was noted with IC50 values for artemether (mean IC50 (95% CI) values of 8.7 (5.9 - 11.6) versus 16.3 (10.7 - 21.8) nM with a mean increase of 87%; p = 0.0068). Previously described SNPs in pfmdr1 are also associated with differences in sensitivity to some artemisinins. CONCLUSIONS: These findings were further explored in molecular modelling experiments that suggest mutations in pfatp6 are unlikely to affect differential binding of artemisinins at their proposed site, whereas there may be differences in such binding associated with mutations in pfmdr1. Implications for a hypothesis that artemisinin resistance may be exacerbated by interactions between PfATP6 and PfMDR1 and for epidemiological studies to monitor emerging resistance are discussed.

  10. Lower head failure analysis

    International Nuclear Information System (INIS)

    Rempe, J.L.; Thinnes, G.L.; Allison, C.M.; Cronenberg, A.W.

    1991-01-01

    The US Nuclear Regulatory Commission is sponsoring a lower vessel head research program to investigate plausible modes of reactor vessel failure in order to determine (a) which modes have the greatest likelihood of occurrence during a severe accident and (b) the range of core debris and accident conditions that lead to these failures. This paper presents the methodology and preliminary results of an investigation of reactor designs and thermodynamic conditions using analytic closed-form approximations to assess the important governing parameters in non-dimensional form. Preliminary results illustrate the importance of vessel and tube geometrical parameters, material properties, and external boundary conditions on predicting vessel failure. Thermal analyses indicate that steady-state temperature distributions will occur in the vessel within several hours, although the exact time is dependent upon vessel thickness. In-vessel tube failure is governed by the tube-to-debris mass ratio within the lower head, where most penetrations are predicted to fail if surrounded by molten debris. Melt penetration distance is dependent upon the effective flow diameter of the tube. Molten debris is predicted to penetrate through tubes with a larger effective flow diameter, such as a boiling water reactor (BWR) drain nozzle. Ex-vessel tube failure for depressurized reactor vessels is predicted to be more likely for a BWR drain nozzle penetration because of its larger effective diameter. At high pressures (between ∼0.1 MPa and ∼12 MPa) ex-vessel tube rupture becomes a dominant failure mechanism, although tube ejection dominates control rod guide tube failure at lower temperatures. However, tube ejection and tube rupture predictions are sensitive to the vessel and tube radial gap size and material coefficients of thermal expansion

  11. Dependent failures of diesel generators

    International Nuclear Information System (INIS)

    Mankamo, T.; Pulkkinen, U.

    1982-01-01

    This survey of dependent failures (common-cause failures) is based on the data of diesel generator failures in U. S. nuclear power plants as reported in Licensee Event Reports. Failures were classified into random and potentially dependent failures. All failures due to design errors, manufacturing or installation errors, maintenance errors, or deviations in the operational environment were classified as potentially dependent failures.The statistical dependence between failures was estimated from the relative portion of multiple failures. Results confirm the earlier view of the significance of statistical dependence, a strong dependence on the age of the diesel generator was found in each failure class excluding random failures and maintenance errors, which had a nearly constant frequency independent of diesel generator age

  12. Assessment of TRAC-PF1/MOD1 against a loss-of-grid transient in Ringhals 4 power plant

    International Nuclear Information System (INIS)

    Sjoberg, A.; Almberger, J.; Sandervag, O.

    1989-07-01

    A loss of grid transient in a three loop Westinghouse PWR has been simulated with the frozen version of TRAC-PF1/MOD1 computer code. The results reveal the capability of the code to qualitatively predict the different pertinent phenomena and the data comparison was quite encouraging. Accurate predictions of the system response required careful determination of the boundary conditions simulating the turbine governor valves and steam dump valves behaviour. An explicit modeling of the steam generator internals was also found to be important for the results. It was also revealed that the pressurizer system including spray and heaters and their operation should be modeled in some detail for proper response. 4 refs., 18 figs

  13. Investigation of the microstructures of ion beams emitted from PF-1000 at different angles to the Z-axis

    Energy Technology Data Exchange (ETDEWEB)

    Skladnik-Sadowska, E.; Czaus, K.; Malinowski, K.; Kwiatkowski, R.; Zebrowski, J. [The Andrzej Soltan Institute for Nuclear Studies, IPJ, 05-400 Otwock-Swierk (Poland); Sadowski, M.J. [The Andrzej Soltan Institute for Nuclear Studies, IPJ, 0R-400 Otwock-Swierk (Poland)] [Institute of Plasma Physics and Laser Microfusion, IPPLM, 01-497 Warsaw (Poland); Paduch, M.; Scholz, M. [Institute of Plasma Physics and Laser Microfusion, IPPLM, 01-497 Warsaw (Poland); Kubes, P. [Czech Technical University, CVUT, 166-27 Prague (Czech Republic); Garkusha, I.E. [Institute of Plasma Physics, NSC KIPT, 61-108 Kharkov (Ukraine); Talebitaher, A. [Plasma Radiation Sources Laboratory, NIE NTU, 637616 Singapore (Singapore)

    2011-07-01

    The paper describes diagnostics of fast ion beams emitted from the large PF-1000 facility operated at 21-27 kV, 290-480 kJ. The use was made of pinhole cameras equipped with PM-355 nuclear track detectors and placed at different angles to the discharge axis. The ion measurements performed at 0 degree angle, as well as those at 60 degrees angle showed a complex spatial structure of the fast ion beams. The ion measurements, which were for the first time performed in the upstream direction (at 180 degrees angle), have proved that some fast deuteron beams are emitted also in the upstream direction. This document is composed of a paper and a poster. (authors)

  14. Full Scale Deposition Trials at 150 MWe PF-boiler Co-firing COal and Straw: Summary of Results

    DEFF Research Database (Denmark)

    Andersen, Karin Hedebo; Frandsen, Flemming; Hansen, Peter Farkas Binderup

    1999-01-01

    A conventional PF-fired boiler at the Danish energy company I/S Midtkraft has been converted to coal-straw co-combustion and a two-year demonstration programme was initiated in January 1996 addressing several aspects of coal-straw co-combustion. Deposition trials were performed as part...... during co-combustion with straw. In addition, where Fe dominated upstream deposits are found in the hottest positions during pure coal combustion, Ca, and to some degree Si, are playing the major role during co-combustion. The addition of straw to the fuel is also seen to lead to a change in the texture...... of the upstream deposits, from an ordered dendritic structure of the larger particles with small particles in between during pure coal combustion, to a more random deposition of the larger particles among the small during co-combustion. No deposition of chlorine species was observed in the SEM-EDX analysis...

  15. Geographic structuring of the Plasmodium falciparum sarco(endoplasmic reticulum Ca2+ ATPase (PfSERCA gene diversity.

    Directory of Open Access Journals (Sweden)

    Ronan Jambou

    Full Text Available Artemisinin, a thapsigargin-like sesquiterpene has been shown to inhibit the Plasmodium falciparum sarco/endoplasmic reticulum calcium-ATPase PfSERCA. To collect baseline pfserca sequence information before field deployment of Artemisinin-based Combination therapies that may select mutant parasites, we conducted a sequence analysis of 100 isolates from multiple sites in Africa, Asia and South America. Coding sequence diversity was large, with 29 mutated codons, including 32 SNPs (average of one SNP/115 bp, of which 19 were novel mutations. Most SNP detected in this study were clustered within a region in the cytosolic head of the protein. The PfSERCA functional domains were very well conserved, with non synonymous mutations located outside the functional domains, except for the S769N mutation associated in French Guiana with elevated IC(50 for artemether. The S769N mutation is located close to the hinge of the headpiece, which in other species modulates calcium affinity and in consequence efficacy of inhibitors, possibly linking calcium homeostasis to drug resistance. Genetic diversity was highest in Senegal, Brazil and French Guiana, and few mutations were identified in Asia. Population genetic analysis was conducted for a partial fragment of the gene encompassing nucleotide coordinates 87-2862 (unambiguous sequence available for 96 isolates. This supported a geographic clustering, with a separation between Old and New World samples and one dominant ancestral haplotype. Genetic drift alone cannot explain the observed polymorphism, suggesting that other evolutionary mechanisms are operating. One possible contributor could be the frequency of haemoglobinopathies that are associated with calcium dysregulation in the erythrocyte.

  16. A series of structurally simple chloroquine chemosensitizing dibemethin derivatives that inhibit chloroquine transport by PfCRT.

    Science.gov (United States)

    Zishiri, Vincent K; Hunter, Roger; Smith, Peter J; Taylor, Dale; Summers, Robert; Kirk, Kiaran; Martin, Rowena E; Egan, Timothy J

    2011-05-01

    A series of 12 new dibemethin (N-benzyl-N-methyl-1-phenylmethanamine) derivatives bearing an N-aminomethyl group attached to the one phenyl ring and an H, Cl, OCH3 or N(CH3)2 group on the other have been synthesized. These compounds all showed strong chloroquine chemosensitizing activity, comparable to verapamil, when present at 1 μM in an in vitro culture of the chloroquine-resistant W2 strain of the human malaria parasite, Plasmodium falciparum. Their N-formylated derivatives also exhibited resistance-reversing activity, but only at substantially higher IC10 concentrations. A number of the dibemethin derivatives were shown to inhibit chloroquine transport via the parasite's 'chloroquine resistance transporter' (PfCRT) in a Xenopus laevis oocyte expression system. The reduced resistance-reversing activity of the formylated compounds relative to their free amine counterparts can probably be ascribed to two factors: decreased accumulation of the formylated dibemethins within the parasite's internal digestive vacuole (believed to be the site of action of chloroquine), and a reduced ability to inhibit PfCRT. The resistance-reversing activity of the compounds described here demonstrates that the amino group need not be attached to the two aromatic rings via a three or four carbon chain as has been suggested by previous QSAR studies. These compounds may be useful as potential side chains for attaching to a 4,7-dichloroquinoline group in order to generate new resistance-reversing chloroquine analogues with inherent antimalarial activity. Copyright © 2011 Elsevier Masson SAS. All rights reserved.

  17. Evaluation of inductive heating energy of a PF insert coil conductor by the calorimetric method (Contract research)

    International Nuclear Information System (INIS)

    Matsui, Kunihiro; Nabara, Yoshihiro; Nunoya, Yoshihiko; Koizumi, Norikiyo; Okuno, Kiyoshi

    2009-02-01

    The PF Insert Coil is a single layer solenoid coil using a superconducting conductor designed for ITER, housed in a Poloidal field coil and installed in the bore of the CS Model Coil. A stability test of the conductor will be performed in a magnetic field generated by the CS Model Coil. In this test, the inductive heat of an inductive heater attached to the conductor will be applied to initiate a normal zone in the conductor. Since the conductor for the PF Insert Coil is a cable-in-conduit conductor, it is quite difficult to estimate inductive heating energy theoretically. Thus, the inductive heating energy is measured experimentally by the calorimetric method. The heating energy is in proportion to a constant multiplied by the integrated square of an applied sinusoidal current wave over the heating period. Experimental results show that the proportional constants of the conductor, cable, conduit and dummy conductor are 0.138 [J/A 2 s], 0.028 [J/A 2 s], 0.118 [J/A 2 s] and 0.009 [J/A 2 s], respectively. The first three denote not only the inductive heating but also the joule heating of the inductive heater. The final value denotes joule heating only. Therefore, subtracting the first three constants by the last one, the proportional constants of inductive heating generated in the conductor, cable and conduit are estimated to be 0.129 [J/A 2 s], 0.019 [J/A 2 s] and 0.109 [J/A 2 s], respectively. (author)

  18. Nutrition in Heart Failure

    Directory of Open Access Journals (Sweden)

    Reci Meseri

    2013-10-01

    Full Text Available Heart failure is defined as decreased ability of heart due to various reasons. It%u2019s seen 2-3% but the prevalence increases sharply after the age of seventy. The objectives of nutrition therapy in heart failure are to prevent from water retention and edema, to avoid from hard digestion and to offer a balanced diet. In order to avoid fluid retention and edema, daily sodium and fluid intake must be monitored carefully. Main dilemma of the heart failure patients is the obesity-cachexia dilemma. Since one of the main reasons of heart failure is cardiovascular diseases, in first phase, the patient may be obese. In the later phases, cachexia may show up. It was shown that cachexia is associated with mortality. Within this period, patients should not be over-fed and the patient should pass from catabolic state to anabolic state slowly. If the gastrointestinal track is functional oral/enteral feeding must be preferred. Multi vitamin and mineral supportsmay be beneficial, which may replace the increased loss, increase anti-inflammatory response and be anti-oxidants. Large, controlled and well-designed studies must be conducted in order to evaluate the benefits of nutritional practices such as nutritional assessment, enteral feeding and nutrient supports in heart failure patients.

  19. A review: Fly ash and deposit formation in PF fired biomass boilers

    DEFF Research Database (Denmark)

    Jensen, Peter Arendt; Jappe Frandsen, Flemming; Wu, Hao

    2016-01-01

    In recent years suspension fired boilers have been increasingly used for biomass based heat and power production in several countries. This has included co-firing of coal and straw, up to 100% firing of wood or straw and the use of additives to remedy problems with biomass firing. In parallel...

  20. Chloroethene Biodegradation Potential, ADOT/PF Peger Road Maintenance Facility, Fairbanks, Alaska

    Science.gov (United States)

    Bradley, Paul M.; Chapelle, Frances H.

    2004-01-01

    A series of 14C-radiotracer-based microcosm experiments were conducted to assess: 1) the extent, rate and products of microbial dechlorination of trichloroethene (TCE), cis-dichloroethene (cis-DCE) and vinyl chloride (VC) in sediments at the Peger Road site; 2) the effect of three electron donor amendments (molasses, shrimp and crab chitin, and 'Hydrogen Release Compound' (HRC)) on microbial degradation of TCE in three Peger Road sediments; and 3) the potential significance at the site of chloroethene biodegradation processes other than reductive dechlorination. In these experiments, TCE biodegradation yielded the reduced products, DCE and VC, and the oxidation product CO 2. Biodegradation of DCE and VC involved stoichiometric oxidation to CO 2. Both laboratory microcosm study and field redox assessment results indicated that the predominant terminal electron accepting process in Peger Road plume sediments under anoxic conditions was Mn/Fe-reduction. The rates of chloroethene biodegradation observed in Peger Road sediment microcosms under low temperature conditions (4?C) were within the range of those observed in sediments from temperate (20?C) aquifer systems. This result confirmed that biodegradation can be a significant mechanism for in situ contaminant remediation even in cold temperature aquifers. The fact that CO2 was the sole product of cis-DCE and VC biodegradation detected in Peger Road sediments indicated that a natural attenuation assessment based on reduced daughter product accumulation may significantly underestimate the potential for DCE and VC biodegradation at the Peger Road. Neither HRC nor molasses addition stimulated TCE reductive dechlorination. The fact that molasses and HRC amendment did stimulate Mn/Fe-reduction suggests that addition of these electron donors favored microbial Mn/Fe-reduction to the detriment of microbial TCE dechlorinating activity. In contrast, amendment of sediment microcosms with shrimp and crab chitin resulted in the

  1. Ductile failure modeling

    DEFF Research Database (Denmark)

    Benzerga, Ahmed Amine; Leblond, Jean Baptiste; Needleman, Alan

    2016-01-01

    Ductile fracture of structural metals occurs mainly by the nucleation, growth and coalescence of voids. Here an overview of continuum models for this type of failure is given. The most widely used current framework is described and its limitations discussed. Much work has focused on extending void...... growth models to account for non-spherical initial void shapes and for shape changes during growth. This includes cases of very low stress triaxiality, where the voids can close up to micro-cracks during the failure process. The void growth models have also been extended to consider the effect of plastic...... anisotropy, or the influence of nonlocal effects that bring a material size scale into the models. Often the voids are not present in the material from the beginning, and realistic nucleation models are important. The final failure process by coalescence of neighboring voids is an issue that has been given...

  2. Structures of three members of Pfam PF02663 (FmdE) implicated in microbial methanogenesis reveal a conserved α+β core domain and an auxiliary C-terminal treble-clef zinc finger

    International Nuclear Information System (INIS)

    Axelrod, Herbert L.; Das, Debanu; Abdubek, Polat; Astakhova, Tamara; Bakolitsa, Constantina; Carlton, Dennis; Chen, Connie; Chiu, Hsiu-Ju; Clayton, Thomas; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Farr, Carol L.; Feuerhelm, Julie; Grant, Joanna C.; Grzechnik, Anna; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Kozbial, Piotr; Krishna, S. Sri; Kumar, Abhinav; Lam, Winnie W.; Marciano, David; McMullan, Daniel; Miller, Mitchell D.; Morse, Andrew T.; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Puckett, Christina; Reyes, Ron; Sefcovic, Natasha; Tien, Henry J.; Trame, Christine B.; Bedem, Henry van den; Weekes, Dana; Wooten, Tiffany; Xu, Qingping; Hodgson, Keith O.; Wooley, John; Elsliger, Marc-André; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.

    2010-01-01

    The first structures from the FmdE Pfam family (PF02663) reveal that some members of this family form tightly intertwined dimers consisting of two domains (N-terminal α+β core and C-terminal zinc-finger domains), whereas others contain only the core domain. The presence of the zinc-finger domain suggests that some members of this family may perform functions associated with transcriptional regulation, protein–protein interaction, RNA binding or metal-ion sensing. Examination of the genomic context for members of the FmdE Pfam family (PF02663), such as the protein encoded by the fmdE gene from the methanogenic archaeon Methanobacterium thermoautotrophicum, indicates that 13 of them are co-transcribed with genes encoding subunits of molybdenum formylmethanofuran dehydrogenase (EC 1.2.99.5), an enzyme that is involved in microbial methane production. Here, the first crystal structures from PF02663 are described, representing two bacterial and one archaeal species: B8FYU2-DESHY from the anaerobic dehalogenating bacterium Desulfitobacterium hafniense DCB-2, Q2LQ23-SYNAS from the syntrophic bacterium Syntrophus aciditrophicus SB and Q9HJ63-THEAC from the thermoacidophilic archaeon Thermoplasma acidophilum. Two of these proteins, Q9HJ63-THEAC and Q2LQ23-SYNAS, contain two domains: an N-terminal thioredoxin-like α+β core domain (NTD) consisting of a five-stranded, mixed β-sheet flanked by several α-helices and a C-terminal zinc-finger domain (CTD). B8FYU2-DESHY, on the other hand, is composed solely of the NTD. The CTD of Q9HJ63-THEAC and Q2LQ23-SYNAS is best characterized as a treble-clef zinc finger. Two significant structural differences between Q9HJ63-THEAC and Q2LQ23-SYNAS involve their metal binding. First, zinc is bound to the putative active site on the NTD of Q9HJ63-THEAC, but is absent from the NTD of Q2LQ23-SYNAS. Second, whereas the structure of the CTD of Q2LQ23-SYNAS shows four Cys side chains within coordination distance of the Zn atom, the structure

  3. Modeling Epidemic Network Failures

    DEFF Research Database (Denmark)

    Ruepp, Sarah Renée; Fagertun, Anna Manolova

    2013-01-01

    This paper presents the implementation of a failure propagation model for transport networks when multiple failures occur resulting in an epidemic. We model the Susceptible Infected Disabled (SID) epidemic model and validate it by comparing it to analytical solutions. Furthermore, we evaluate...... the SID model’s behavior and impact on the network performance, as well as the severity of the infection spreading. The simulations are carried out in OPNET Modeler. The model provides an important input to epidemic connection recovery mechanisms, and can due to its flexibility and versatility be used...... to evaluate multiple epidemic scenarios in various network types....

  4. Echo and heart failure

    DEFF Research Database (Denmark)

    Modin, Daniel; Andersen, Ditte Madsen; Biering-Sørensen, Tor

    2018-01-01

    Heart failure (HF) is a threat to public health. Heterogeneities in aetiology and phenotype complicate the diagnosis and management of HF. This is especially true when considering HF with preserved ejection fraction (HFpEF), which makes up 50% of HF cases. Natriuretic peptides may aid in establis......Heart failure (HF) is a threat to public health. Heterogeneities in aetiology and phenotype complicate the diagnosis and management of HF. This is especially true when considering HF with preserved ejection fraction (HFpEF), which makes up 50% of HF cases. Natriuretic peptides may aid...

  5. Generalized Block Failure

    DEFF Research Database (Denmark)

    Jönsson, Jeppe

    2015-01-01

    Block tearing is considered in several codes as a pure block tension or a pure block shear failure mechanism. However in many situations the load acts eccentrically and involves the transfer of a substantial moment in combination with the shear force and perhaps a normal force. A literature study...... shows that no readily available tests with a well-defined substantial eccentricity have been performed. This paper presents theoretical and experimental work leading towards generalized block failure capacity methods. Simple combination of normal force, shear force and moment stress distributions along...... yield lines around the block leads to simple interaction formulas similar to other interaction formulas in the codes....

  6. Factores de éxito y fracaso en el comportamiento innovador de las empresas ganaderas cubanas Success and failure factors in the innovating performance of the Cuban livestock production enterprises

    Directory of Open Access Journals (Sweden)

    J Suárez

    2007-09-01

    de éxito y fracaso de la innovación en el sector ganadero cubano son muy similares a los obtenidos en el sector industrial y referidos en la literatura internacionalWith the objective of learning the factors that favor the success of innovation and those that cause its failure, in the Cuban livestock production sector, a questionnaire was applied to the directors of a non probabilistic sample of 34 livestock production enterprises (36% of the sector, which comprised the different geographical zones of the country, all the types of production systems existing in Cuban livestock production, organizations in the different stages of the Enterprise Improvement process and the different enterprise sizes. The internal factors with the highest incidence on the success of innovation in the enterprises were considered to be: A Top Management opened to new ideas and inclined to assuming risks, the decentralization in decision-making and a participatory management, motivation and commitment to innovation in the managers, constant training of the personnel and execution of team work; while the external factors were the dynamics and turbulence of the surroundings, which demand changes in the enterprise, the establishment of cooperation links and networks in the country, the existence of state policies of support to innovation, having facility of access to international funding sources and possibilities of national financing for the development of innovation. Regarding the obstacles that constrain to a higher extent innovation in the enterprises, they are: scarcity of financial resources, external pressures on the enterprise, which have incidence on the productive and management processes, the possession of insufficient scientific and technological information, problems of availability and utilization of computing equipment, lack of efficient financing mechanisms for innovation, as well as insufficient mechanisms of incentives that encourage to innovate and establish links with

  7. Acute liver failure and self-medication.

    Science.gov (United States)

    de Oliveira, André Vitorio Câmara; Rocha, Frederico Theobaldo Ramos; Abreu, Sílvio Romero de Oliveira

    2014-01-01

    Not responsible self-medication refers to drug use in high doses without rational indication and often associated with alcohol abuse. It can lead to liver damage and drug interactions, and may cause liver failure. To warn about how the practice of self-medication can be responsible for acute liver failure. Were used the Medline via PubMed, Cochrane Library, SciELO and Lilacs, and additional information on institutional sites of interest crossing the headings acute liver failure [tiab] AND acetaminophen [tiab]; self-medication [tiab] AND acetaminophen [tiab]; acute liver failure [tiab] AND dietary supplements [tiab]; self-medication [tiab] AND liver failure [tiab] and self-medication [tiab] AND green tea [tiab]. In Lilacs and SciELO used the descriptor self medication in Portuguese and Spanish. From total surveyed were selected 27 articles and five sites specifically related to the purpose of this review. Legislation and supervision disabled and information inaccessible to people, favors the emergence of cases of liver failure drug in many countries. In the list of released drugs that deserve more attention and care, are some herbal medicines used for the purpose of weight loss, and acetaminophen. It is recommended that institutes of health intensify supervision and better orient their populations on drug seemingly harmless, limiting the sale of products or requiring a prescription for release them.

  8. 27 CFR 70.103 - Failure to pay tax.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Failure to pay tax. 70.103... § 70.103 Failure to pay tax. Whoever fails to pay any tax imposed by Part I of Subchapter A of Chapter... penalty of 5 percent of the tax due but unpaid. For additional penalties for failure to pay tax, see 27...

  9. Accelerated Testing with Multiple Failure Modes under Several Temperature Conditions

    OpenAIRE

    Zongyue Yu; Zhiqian Ren; Junyong Tao; Xun Chen

    2014-01-01

    A complicated device may have multiple failure modes, and some of the failure modes are sensitive to low temperatures. To assess the reliability of a product with multiple failure modes, this paper presents an accelerated testing in which both of the high temperatures and the low temperatures are applied. Firstly, an acceleration model based on the Arrhenius model but accounting for the influence of both the high temperatures and low temperatures is proposed. Accordingly, an accelerated testi...

  10. Antibodies against PfEMP1, RIFIN, MSP3 and GLURP are acquired during controlled Plasmodium falciparum malaria infections in naïve volunteers

    DEFF Research Database (Denmark)

    Turner, Louise; Wang, Christian W; Lavstsen, Thomas

    2011-01-01

    Antibodies to polymorphic antigens expressed during the parasites erythrocytic stages are important mediators of protective immunity against P. falciparum malaria. Therefore, polymorphic blood stage antigens like MSP3, EBA-175 and GLURP and variant surface antigens PfEMP1 and RIFIN are considered...

  11. Characterization of the arsenite oxidizer Aliihoeflea sp. strain 2WW and its potential application in the removal of arsenic from groundwater in combination with Pf-ferritin.

    Science.gov (United States)

    Corsini, Anna; Colombo, Milena; Muyzer, Gerard; Cavalca, Lucia

    2015-09-01

    A heterotrophic arsenite-oxidizing bacterium, strain 2WW, was isolated from a biofilter treating arsenic-rich groundwater. Comparative analysis of 16S rRNA gene sequences showed that it was closely related (98.7 %) to the alphaproteobacterium Aliihoeflea aesturari strain N8(T). However, it was physiologically different by its ability to grow at relatively low substrate concentrations, low temperatures and by its ability to oxidize arsenite. Here we describe the physiological features of strain 2WW and compare these to its most closely related relative, A. aestuari strain N8(T). In addition, we tested its efficiency to remove arsenic from groundwater in combination with Pf-ferritin. Strain 2WW oxidized arsenite to arsenate between pH 5.0 and 8.0, and from 4 to 30 °C. When the strain was used in combination with a Pf-ferritin-based material for arsenic removal from natural groundwater, the removal efficiency was significantly higher (73 %) than for Pf-ferritin alone (64 %). These results showed that arsenite oxidation by strain 2WW combined with Pf-ferritin-based material has a potential in arsenic removal from contaminated groundwater.

  12. Structure-guided identification of a family of dual receptor-binding PfEMP1 that is associated with cerebral malaria

    DEFF Research Database (Denmark)

    Lennartz, Frank; Adams, Yvonne; Bengtsson, Anja

    2017-01-01

    Cerebral malaria is a deadly outcome of infection by Plasmodium falciparum, occurring when parasite-infected erythrocytes accumulate in the brain. These erythrocytes display parasite proteins of the PfEMP1 family that bind various endothelial receptors. Despite the importance of cerebral malaria...

  13. Mapping the binding site of a cross-reactive Plasmodium falciparum PfEMP1 monoclonal antibody inhibitory of ICAM-1 binding

    DEFF Research Database (Denmark)

    Lennartz, Frank; Bengtsson, Anja; Olsen, Rebecca W

    2015-01-01

    The virulence of Plasmodium falciparum is linked to the ability of infected erythrocytes (IE) to adhere to the vascular endothelium, mediated by P. falciparum erythrocyte membrane protein 1 (PfEMP1). In this article, we report the functional characterization of an mAb that recognizes a panel of P...

  14. Low-frequency permittivity of spin-density wave in (TMTSF)2PF6 at low temperatures

    DEFF Research Database (Denmark)

    Nad, F.; Monceau, P.; Bechgaard, K.

    1995-01-01

    Conductivity and permittivity epsilon of(TMTSF)(2)PF6 have been measured at low frequencies of (10(2)-10(7) Hz) at low temperatures below the spin-density wave (SDW) transition temperature T-p. The temperature dependence of the conductivity shows a deviation from thermally activated behavior at T...

  15. PF-05231023, a long-acting FGF21 analogue, decreases body weight by reduction of food intake in non-human primates.

    Science.gov (United States)

    Thompson, W Clayton; Zhou, Yingjiang; Talukdar, Saswata; Musante, Cynthia J

    2016-08-01

    PF-05231023, a long-acting FGF21 analogue, is a promising potential pharmacotherapy for the treatment of obesity and associated comorbidities. Previous studies have shown the potential of FGF21 and FGF21-like compounds to decrease body weight in mice, non-human primates, and humans; the precise mechanisms of action remain unclear. In particular, there have been conflicting reports on the degree to which FGF21-induced weight loss in non-human primates is attributable to a decrease in food intake versus an increase in energy expenditure. Here, we present a semi-mechanistic mathematical model of energy balance and body composition developed from similar work in mice. This model links PF-05231023 administration and washout to changes in food intake, which in turn drives changes in body weight. The model is calibrated to and compared with recently published data from cynomolgus macaques treated with PF-05231023, demonstrating its accuracy in describing pharmacotherapy-induced weight loss in these animals. The results are consistent with the hypothesis that PF-05231023 decreases body weight in cynomolgus macaques solely by a reduction in food intake, with no direct effect on energy expenditure.

  16. Assessment of TRAC-PF1/MOD3 Mark-22 assembly model using SRL ''A'' tank single-assembly flow experiments

    International Nuclear Information System (INIS)

    Fischer, S.R.; Lam, K.; Lin, J.C.

    1991-01-01

    This paper summarizes the results of an assessment of our TRAC-PF1/MOD3 Mark-22 prototype fuel assembly model against single-assembly data obtained from the ''A'' Tank single-assembly tests that were performed at the Savannah River Laboratory. We felt the data characterize prototypic assembly behavior over a range of air-water flow conditions of interest for loss-of-coolant accident (LOCA) calculations. This study was part of a benchmarking effort performed to evaluate and validate a multiple-assembly, full-plant model that is being developed by Los Alamos National Laboratory to study various aspects of the Savannah River plant operating conditions, including LOCA transients, using TRAC-PF1/MOD3 Version 1.10. The results of this benchmarking effort demonstrate that TRAC-PF1/MOD3 is capable pf calculating plenum conditions and assembly flows during conditions thought to be typical of the Emergency Cooling System (ECS) phase of a LOCA. 10 refs., 12 fig

  17. Refurbishment and modification of existing protective shipping packages (for 30-inch UF{sub 6} cylinders) per USDOT specification No. USA-DOT-21PF-1A

    Energy Technology Data Exchange (ETDEWEB)

    Housholder, W.R. [Nuclear Containers, Incorporated, Elizabethton, TN (United States)

    1991-12-31

    This paper addresses the refurbishment procedures for existing shipping containers for 30-inch diameter UF{sub 6} cylinders in accordance with DOT Specification 21PF-1 and the criteria used to determine rejection when such packages are unsuitable for refurbishment.

  18. Antibody reactivity to conserved linear epitopes of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1)

    DEFF Research Database (Denmark)

    Staalsø, T; Khalil, E A; Elhassan, I M

    1998-01-01

    recognising the conserved regions of PfEMP1 arise upon exposure to the parasite, and that these may be involved in the development of protection against malaria. Antibodies to the Pfalhesin peptide of the human aniontransporter, band3, were measured by the same method. The magnitude of this antibody response...

  19. Antibodies to ICAM1-binding PfEMP1-DBLβ are biomarkers of protective immunity to malaria in a cohort of young children from Papua New Guinea

    DEFF Research Database (Denmark)

    Tessema, Sofonias K; Utama, Digjaya; Chesnokov, Olga

    2018-01-01

    Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) mediates parasite sequestration to the cerebral microvasculature via binding of DBLβ domains to Intercellular Adhesion Molecule 1 (ICAM1) and is associated with severe cerebral malaria. In a cohort of 187 young children from Papua New ...

  20. Surface co-expression of two different PfEMP1 antigens on single Plasmodium falciparum-infected erythrocytes facilitates binding to ICAM1 and PECAM1

    DEFF Research Database (Denmark)

    Joergensen, Louise; Bengtsson, Dominique C; Bengtsson, Anja

    2010-01-01

    The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigens play a major role in cytoadhesion of infected erythrocytes (IE), antigenic variation, and immunity to malaria. The current consensus on control of variant surface antigen expression is that only one PfEMP1 encoded by one var...

  1. Isogeometric failure analysis

    NARCIS (Netherlands)

    Verhoosel, C.V.; Scott, M.A.; Borden, M.J.; Borst, de R.; Hughes, T.J.R.; Mueller-Hoeppe, D.; Loehnert, S.; Reese, S.

    2011-01-01

    Isogeometric analysis is a versatile tool for failure analysis. On the one hand, the excellent control over the inter-element continuity conditions enables a natural incorporation of continuum constitutive relations that incorporate higher-order strain gradients, as in gradient plasticity or damage.

  2. Beyond Success and Failure

    NARCIS (Netherlands)

    Etalle, Sandro; Jaffar, Joxan; van Raamsdonk, Femke

    We study a new programming framework based on logic programming where success and failure are replaced by predicates for adequacy and inadequacy. Adequacy allows to extract a result from a partial computation, and inadequacy allows to flexibly constrain the search space. In this parameterized

  3. Poverty and Aspirations Failure

    NARCIS (Netherlands)

    Dalton, P.S.; Ghosal, S.; Mani, A.

    2011-01-01

    We develop a theoretical framework to study the psychology of poverty and 'aspirations failure'. In our framework, the rich and the poor share the same preferences - and also a behavioral bias in setting aspirations. Greater downside risks imposed by poverty exacerbates the effects of this

  4. Poverty and aspirations failure

    NARCIS (Netherlands)

    Dalton, P.S.; Ghosal, S.; Mani, A.

    We develop a theoretical framework to study the psychology of poverty and ‘aspirations failure’, defined as the failure to aspire to one’s own potential. In our framework, rich and the poor persons share the same preferences and same behavioral bias in setting aspirations. We show that poverty can

  5. Copeptin in Heart Failure

    DEFF Research Database (Denmark)

    Balling, Louise; Gustafsson, Finn

    2016-01-01

    Heart failure (HF) is one of the most common causes of hospitalization and mortality in the modern Western world and an increasing proportion of the population will be affected by HF in the future. Although HF management has improved quality of life and prognosis, mortality remains very high...

  6. Acute liver failure

    DEFF Research Database (Denmark)

    Bernal, William; Lee, William M; Wendon, Julia

    2015-01-01

    Over the last three decades acute liver failure (ALF) has been transformed from a rare and poorly understood condition with a near universally fatal outcome, to one with a well characterized phenotype and disease course. Complex critical care protocols are now applied and emergency liver...

  7. Premature ovarian failure

    OpenAIRE

    Pacheco, José

    2011-01-01

    Premature ovarian failure is characterized by secondary amenorrhea affecting a woman before the age of 40, leading to hypoestrogenism, infertility, and consequences of premature menopause, such as osteoporosis, cardiovascular disease, neurovegetative alterations, and others. Follicular exhaustion is due to either follicles shortage or oocytes accelerated destruction. Main causes are genetic, autoimmune and iatrogenic. Among genetic causes Xq and Xp deletions, translocations, numeric aberratio...

  8. CMR in Heart Failure.

    OpenAIRE

    Sado, D. M.; Hasleton, J. M.; Herrey, A. S.; Moon, J. C.

    2011-01-01

    Heart Failure (HF) is a common syndrome with multiple causes. Cardiovascular magnetic resonance (CMR) is a medical imaging technique with significant advantages, allowing the understanding of aetiology and pathophysiology of HF in the individual patient, permitting specific therapy to be administered and predicting prognosis. This paper discusses the diverse role of CMR in HF.

  9. Perioperative acute renal failure.

    LENUS (Irish Health Repository)

    Mahon, Padraig

    2012-02-03

    PURPOSE OF REVIEW: Recent biochemical evidence increasingly implicates inflammatory mechanisms as precipitants of acute renal failure. In this review, we detail some of these pathways together with potential new therapeutic targets. RECENT FINDINGS: Neutrophil gelatinase-associated lipocalin appears to be a sensitive, specific and reliable biomarker of renal injury, which may be predictive of renal outcome in the perioperative setting. For estimation of glomerular filtration rate, cystatin C is superior to creatinine. No drug is definitively effective at preventing postoperative renal failure. Clinical trials of fenoldopam and atrial natriuretic peptide are, at best, equivocal. As with pharmacological preconditioning of the heart, volatile anaesthetic agents appear to offer a protective effect to the subsequently ischaemic kidney. SUMMARY: Although a greatly improved understanding of the pathophysiology of acute renal failure has offered even more therapeutic targets, the maintenance of intravascular euvolaemia and perfusion pressure is most effective at preventing new postoperative acute renal failure. In the future, strategies targeting renal regeneration after injury will use bone marrow-derived stem cells and growth factors such as insulin-like growth factor-1.

  10. Diuretics for heart failure.

    Science.gov (United States)

    Faris, Rajaa F; Flather, Marcus; Purcell, Henry; Poole-Wilson, Philip A; Coats, Andrew J S

    2012-02-15

    Chronic heart failure is a major cause of morbidity and mortality worldwide. Diuretics are regarded as the first-line treatment for patients with congestive heart failure since they provide symptomatic relief. The effects of diuretics on disease progression and survival remain unclear. To assess the harms and benefits of diuretics for chronic heart failure Updated searches were run in the Cochrane Central Register of Controlled Trials in The Cochrane Library (CENTRAL Issue 1 of 4, 2011), MEDLINE (1966 to 22 February 2011), EMBASE (1980 to 2011 Week 07) and HERDIN database (1990 to February 2011). We hand searched pertinent journals and reference lists of papers were inspected. We also contacted manufacturers and researchers in the field. No language restrictions were applied. Double-blinded randomised controlled trials of diuretic therapy comparing one diuretic with placebo, or one diuretic with another active agent (e.g. ACE inhibitors, digoxin) in patients with chronic heart failure. Two authors independently abstracted the data and assessed the eligibility and methodological quality of each trial. Extracted data were analysed by determining the odds ratio for dichotomous data, and difference in means for continuous data, of the treated group compared with controls. The likelihood of heterogeneity of the study population was assessed by the Chi-square test. If there was no evidence of statistical heterogeneity and pooling of results was clinically appropriate, a combined estimate was obtained using the fixed-effects model. This update has not identified any new studies for inclusion. The review includes 14 trials (525 participants), 7 were placebo-controlled, and 7 compared diuretics against other agents such as ACE inhibitors or digoxin. We analysed the data for mortality and for worsening heart failure. Mortality data were available in 3 of the placebo-controlled trials (202 participants). Mortality was lower for participants treated with diuretics than for

  11. Empirical parameters for solvent acidity, basicity, dipolarity, and polarizability of the ionic liquids [BMIM][BF4] and [BMIM][PF6].

    Science.gov (United States)

    del Valle, J C; García Blanco, F; Catalán, J

    2015-04-02

    The empirical solvent scales for polarizability (SP), dipolarity (SdP), acidity (SA), and basicity (SB) have been successfully used to interpret the solvatochromism of compounds dissolved in organic solvents and their solvent mixtures. Providing that the published solvatochromic parameters for the ionic liquids 1-(1-butyl)-3-methylimidazolium tetrafluoroborate, [BMIM][BF4] and 1-(1-butyl)-3-methylimidazolium hexafluorophosphate, [BMIM][PF6], are excessively widespread, their SP, SdP, SA, and SB values are measured herein at temperatures from 293 to 353 K. Four key points are emphasized herein: (i) the origin of the solvatochromic solvent scales--the gas phase, that is the absence of any medium perturbation--; (ii) the separation of the polarizability and dipolarity effects; (iii) the simplification of the probing process in order to obtain the solvatochromic parameters; and (iv) the SP, SdP, SA, and SB solvent scales can probe the polarizability, dipolarity, acidity, and basicity of ionic liquids as well as of organic solvents and water-organic solvent mixtures. From the multiparameter approach using the four pure solvent scales one can draw the conclusion that (a) the solvent influence of [BMIM][BF4] parallels that of formamide at 293 K, both of them miscible with water; (b) [BMIM][PF6] shows a set of solvatochromic parameters similar to that of chloroacetonitrile, both of them water insoluble; and (c) that the corresponding solvent acidity and basicity of the ionic liquids can be explained to a great extent from the cation species by comparing the empirical parameters of [BMIM](+) with those of the solvent 1-methylimidazole. The insolubility of [BMIM][PF6] in water as compared to [BMIM][BF4] is tentatively connected to some extent to the larger molar volume of the anion [PF6](-), and to the difference in basicity of [PF6](-) and [BF4](-).

  12. Reliability analysis based on the losses from failures.

    Science.gov (United States)

    Todinov, M T

    2006-04-01

    The conventional reliability analysis is based on the premise that increasing the reliability of a system will decrease the losses from failures. On the basis of counterexamples, it is demonstrated that this is valid only if all failures are associated with the same losses. In case of failures associated with different losses, a system with larger reliability is not necessarily characterized by smaller losses from failures. Consequently, a theoretical framework and models are proposed for a reliability analysis, linking reliability and the losses from failures. Equations related to the distributions of the potential losses from failure have been derived. It is argued that the classical risk equation only estimates the average value of the potential losses from failure and does not provide insight into the variability associated with the potential losses. Equations have also been derived for determining the potential and the expected losses from failures for nonrepairable and repairable systems with components arranged in series, with arbitrary life distributions. The equations are also valid for systems/components with multiple mutually exclusive failure modes. The expected losses given failure is a linear combination of the expected losses from failure associated with the separate failure modes scaled by the conditional probabilities with which the failure modes initiate failure. On this basis, an efficient method for simplifying complex reliability block diagrams has been developed. Branches of components arranged in series whose failures are mutually exclusive can be reduced to single components with equivalent hazard rate, downtime, and expected costs associated with intervention and repair. A model for estimating the expected losses from early-life failures has also been developed. For a specified time interval, the expected losses from early-life failures are a sum of the products of the expected number of failures in the specified time intervals covering the

  13. Coding hazardous tree failures for a data management system

    Science.gov (United States)

    Lee A. Paine

    1978-01-01

    Codes for automatic data processing (ADP) are provided for hazardous tree failure data submitted on Report of Tree Failure forms. Definitions of data items and suggestions for interpreting ambiguously worded reports are also included. The manual is intended to insure the production of accurate and consistent punched ADP cards which are used in transfer of the data to...

  14. ▼ Sacubitril valsartan for heart failure.

    Science.gov (United States)

    2016-06-01

    ▼ Sacubitril valsartan (Entresto-Novartis) is a new oral drug licensed for the treatment of symptomatic chronic heart failure in adults with reduced ejection fraction.(1) It is described as an angiotensin receptor neprilysin inhibitor and contains the neprilysin inhibitor, sacubitril and the angiotensin II receptor antagonist, valsartan.(1-3) Here, we review the evidence for sacubitril valsartan and consider its place in the management of heart failure. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  15. Treatment pf pig slurry in Spain to minimize soil degradation and environmental impact

    International Nuclear Information System (INIS)

    Espejo Marin, C.; Garcia Marin, R.

    2009-01-01

    The pig farms in Spain have experimented on the last years a great development, giving place to a new productive, sanitary, economic and environmental reality. His intensification has generated the presence of farms of great capacity, which generate high quantities of excrements and residues. Due to the dynamism of the sector, the legislative existing frame until ends of the nineties of the past 20th century remains obsolete. For such motive, the Government of Spain established basic standards of management of pig farms (RD 324/2000); regulatory rules for awarding grants to projects that seek to improve environmental management of pig farms (RD 987/2008); and, in December 2008, is approved, in Ministers council, a plan of bio-digestion of excrements, which has as aim decrease the greenhouse gases and reduce the risk of pollution of soils, fluvial riverbeds and underground aquifers. (Author) 9 refs.

  16. Formulation of microemulsion propolis fluoride (PF) as varnish topical agent to stop activity of teeth caries

    Science.gov (United States)

    Sahlan, Muhamad; Prakoso, Chandra Dwi; Darwita, Risqa Rina; Hermansyah, Heri

    2017-02-01

    Topical fluoride is proven to have higher efficacy in preventing dental caries with low production cost and easy to apply. The objective of this research is to formulate alternative agent topical fluoride NH4F 5% mixed with extract ethanol propolis (EEP) in the micro-emulsion system that has high stability, antimicrobial activity, and remineralization capability to arrest teeth caries activity. By using total plate count (TPC) analysis, formulation 2.7% EEP; 6,3% surfactant; and 90,9% NH4F shows good perform to inhibit cariogenic bacteria development around 78-80%. Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray (EDX) result also showed that sample successfully remineralized enamel surface. In addition, sample showed good pH, flavonoid, and polyphenol stability for 40 days.

  17. High failure rate of trochanteric fracture osteosynthesis with proximal femoral locking compression plate.

    Science.gov (United States)

    Wirtz, C; Abbassi, F; Evangelopoulos, D S; Kohl, S; Siebenrock, K A; Krüger, A

    2013-06-01

    Stable reconstruction of proximal femoral (PF) fractures is especially challenging due to the peculiarity of the injury patterns and the high load-bearing requirement. Since its introduction in 2007, the PF-locking compression plate (LCP) 4.5/5.0 has improved osteosynthesis for intertrochanteric and subtrochanteric fractures of the femur. This study reports our early results with this implant. Between January 2008 and June 2010, 19 of 52 patients (12 males, 7 females; mean age 59 years, range 19-96 years) presenting with fractures of the trochanteric region were treated at the authors' level 1 trauma centre with open reduction and internal fixation using PF-LCP. Postoperatively, partial weight bearing was allowed for all 19 patients. Follow-up included a thorough clinical and radiological evaluation at 1.5, 3, 6, 12, 24, 36 and 48 months. Failure analysis was based on conventional radiological and clinical assessment regarding the type of fracture, postoperative repositioning, secondary fracture dislocation in relation to the fracture constellation and postoperative clinical function (Merle d'Aubigné score). In 18 patients surgery achieved adequate reduction and stable fixation without intra-operative complications. In one patient an ad latus displacement was observed on postoperative X-rays. At the third month follow-up four patients presented with secondary varus collapse and at the sixth month follow-up two patients had 'cut-outs' of the proximal fragment, with one patient having implant failure due to a broken proximal screw. Revision surgeries were performed in eight patients, one patient receiving a change of one screw, three patients undergoing reosteosynthesis with implantation of a condylar plate and one patient undergoing hardware removal with secondary implantation of a total hip prosthesis. Eight patients suffered from persistent trochanteric pain and three patients underwent hardware removal. Early results for PF-LCP osteosynthesis show major

  18. Immunization with the Malaria Diversity-Covering Blood-Stage Vaccine Candidate Plasmodium falciparum Apical Membrane Antigen 1 DiCo in Complex with Its Natural Ligand PfRon2 Does Not Improve the In Vitro Efficacy

    Directory of Open Access Journals (Sweden)

    Holger Spiegel

    2017-06-01

    Full Text Available The blood-stage malaria vaccine candidate Plasmodium falciparum apical membrane antigen 1 (PfAMA1 can induce strong parasite growth-inhibitory antibody responses in animals but has not achieved the anticipated efficacy in clinical trials. Possible explanations in humans are the insufficient potency of the elicited antibody responses, as well as the high degree of sequence polymorphisms found in the field. Several strategies have been developed to improve the cross-strain coverage of PfAMA1-based vaccines, whereas innovative concepts to increase the potency of PfAMA1-specific IgG responses have received little attention even though this may be an essential requirement for protective efficacy. A previous study has demonstrated that immunization with a complex of PyAMA1 and PyRON2, a ligand with an essential functional role in erythrocyte invasion, leads to protection from lethal Plasmodium yoelli challenge in an animal model and suggested to extend this strategy toward improved strain coverage by using multiple PfAMA1 alleles in combination with PfRon2L. As an alternative approach along this line, we decided to use PfRon2L in combination with three PfAMA1 diversity covering variants (DiCo to investigate the potential of this complex to induce more potent parasite growth inhibitory immune response in combination with better cross-strain-specific efficacy. Within the limits of the study design, the ability of the PfAMA1 DiCo-Mix to induce cross-strain-specific antibodies was not affected in all immunization groups, but the DiCo–PfRon2L complexes did not improve the potency of PfAMA1-specific IgG responses.

  19. OBSTETRIC RENAL FAILURE

    Directory of Open Access Journals (Sweden)

    Rajeshwari

    2015-11-01

    Full Text Available Renal failure in obstetrics is rare but important complication, associated with significant mortality and long term morbidity.1,2 It includes acute renal failure due to obstetrical complications or due to deterioration of existing renal disease. AIMS AND OBJECTIVES: To evaluate the etiology and outcome of renal failure in obstetric patients. METHODS: We prospectively analyzed 30 pregnant and puerperal women with acute renal failure or pre-existing renal disease developing renal failure during pregnancy between November 2007 to sep-2009. Patients who presented/developed ARF during the hospital stay were included in this study. RESULTS: Among 30 patients, mean age was 23 years and 33 years age group. 12 cases (40% patients were primigravidae and 9(30% patients were multigravidae and 9 cases (30% presented in post-partum period. Eighteen cases (60% with ARF were seen in third trimester, followed by in postpartum period 9 cases (30%. Most common contributing factors to ARF were Pre-eclampsia, eclampsia and HELLP syndrome 60%, sepsis 56.6%, post abortal ARF 10%. DIC 40%. Haemorrhage as the aetiology for ARF was present 46%, APH in 20% and PPH in 26.6%. The type of ARF was renal in (63% and prerenal (36%; Oliguric seen in 10 patients (33% and high mortality (30%. Among the 20 pregnant patients with ARF, The average period of gestation was 33±2 weeks (30 -36 weeks, 5 cases (25% presented with intrauterine fetal demise and 18 cases (66% had preterm vaginal delivery and 2 cases (10% had induced abortion. And the average birth weight was 2±0.5 kg (1.5 kg. Eight cases (26% required dialysis. 80% of patients recovered completely of renal functions. 63% patients recovered without renal replacement therapy whereas 17% required dialysis. the maternal mortality was 20%, the main reason for mortality was septic shock and multi organ dysfunction (66%. CONCLUSION: ARF related pregnancy was seen commonly in the primigravidae and in the third trimester, the most

  20. Unified description of pf-shell nuclei by the Monte Carlo shell model calculations

    Energy Technology Data Exchange (ETDEWEB)

    Mizusaki, Takahiro; Otsuka, Takaharu [Tokyo Univ. (Japan). Dept. of Physics; Honma, Michio

    1998-03-01

    The attempts to solve shell model by new methods are briefed. The shell model calculation by quantum Monte Carlo diagonalization which was proposed by the authors is a more practical method, and it became to be known that it can solve the problem with sufficiently good accuracy. As to the treatment of angular momentum, in the method of the authors, deformed Slater determinant is used as the basis, therefore, for making angular momentum into the peculiar state, projected operator is used. The space determined dynamically is treated mainly stochastically, and the energy of the multibody by the basis formed as the result is evaluated and selectively adopted. The symmetry is discussed, and the method of decomposing shell model space into dynamically determined space and the product of spin and isospin spaces was devised. The calculation processes are shown with the example of {sup 50}Mn nuclei. The calculation of the level structure of {sup 48}Cr with known exact energy can be done with the accuracy of peculiar absolute energy value within 200 keV. {sup 56}Ni nuclei are the self-conjugate nuclei of Z=N=28. The results of the shell model calculation of {sup 56}Ni nucleus structure by using the interactions of nuclear models are reported. (K.I.)