Vitamin C induces specific demethylation of H3K9me2 in mouse embryonic stem cells via Kdm3a/b.

Science.gov (United States)

Ebata, Kevin T; Mesh, Kathryn; Liu, Shichong; Bilenky, Misha; Fekete, Alexander; Acker, Michael G; Hirst, Martin; Garcia, Benjamin A; Ramalho-Santos, Miguel

2017-01-01

Histone methylation patterns regulate gene expression and are highly dynamic during development. The erasure of histone methylation is carried out by histone demethylase enzymes. We had previously shown that vitamin C enhances the activity of Tet enzymes in embryonic stem (ES) cells, leading to DNA demethylation and activation of germline genes. We report here that vitamin C induces a remarkably specific demethylation of histone H3 lysine 9 dimethylation (H3K9me2) in naïve ES cells. Vitamin C treatment reduces global levels of H3K9me2, but not other histone methylation marks analyzed, as measured by western blot, immunofluorescence and mass spectrometry. Vitamin C leads to widespread loss of H3K9me2 at large chromosomal domains as well as gene promoters and repeat elements. Vitamin C-induced loss of H3K9me2 occurs rapidly within 24 h and is reversible. Importantly, we found that the histone demethylases Kdm3a and Kdm3b are required for vitamin C-induced demethylation of H3K9me2. Moreover, we show that vitamin C-induced Kdm3a/b-mediated H3K9me2 demethylation and Tet-mediated DNA demethylation are independent processes at specific loci. Lastly, we document Kdm3a/b are partially required for the upregulation of germline genes by vitamin C. These results reveal a specific role for vitamin C in histone demethylation in ES cells and document that DNA methylation and H3K9me2 cooperate to silence germline genes in pluripotent cells.

Covalent Modifications of Histone H3K9 Promote Binding of CHD3

Directory of Open Access Journals (Sweden)

Adam H. Tencer

2017-10-01

Full Text Available Chromatin remodeling is required for genome function and is facilitated by ATP-dependent complexes, such as nucleosome remodeling and deacetylase (NuRD. Among its core components is the chromodomain helicase DNA binding protein 3 (CHD3 whose functional significance is not well established. Here, we show that CHD3 co-localizes with the other NuRD subunits, including HDAC1, near the H3K9ac-enriched promoters of the NuRD target genes. The tandem PHD fingers of CHD3 bind histone H3 tails and posttranslational modifications that increase hydrophobicity of H3K9—methylation or acetylation (H3K9me3 or H3K9ac—enhance this interaction. Binding of CHD3 PHDs promotes H3K9Cme3-nucleosome unwrapping in vitro and perturbs the pericentric heterochromatin structure in vivo. Methylation or acetylation of H3K9 uniquely alleviates the intra-nucleosomal interaction of histone H3 tails, increasing H3K9 accessibility. Collectively, our data suggest that the targeting of covalently modified H3K9 by CHD3 might be essential in diverse functions of NuRD.

Preclinical evaluation of potential infection-imaging probe [68 Ga]Ga-DOTA-K-A9 in sterile and infectious inflammation.

Science.gov (United States)

Nielsen, Karin M; Jørgensen, Nis P; Kyneb, Majbritt H; Borghammer, Per; Meyer, Rikke L; Thomsen, Trine R; Bender, Dirk; Jensen, Svend B; Nielsen, Ole L; Alstrup, Aage K O

2018-05-23

The development of bacteria-specific infection radiotracers is of considerable interest to improve diagnostic accuracy and enabling therapy monitoring. The aim of this study was to determine if the previously reported radiolabelled 1,4,7,10-tetraazacyclododecane-N,N',N″,N‴-tetraacetic acid (DOTA) conjugated peptide, [ 68 Ga]Ga-DOTA-K-A9 could detect a staphylococcal infection in vivo, and distinguish it from aseptic inflammation. An optimised [ 68 Ga]Ga-DOTA-K-A9 synthesis omitting the use of acetone was developed, yielding 93% ± 0.9% radiochemical purity. The in vivo infection binding specificity of [ 68 Ga]Ga-DOTA-K-A9 was evaluated by micro positron emission tomography/magnetic resonance imaging (μPET/MRI) of 15 mice with either subcutaneous S. aureus infection or turpentine induced inflammation and compared with 2-deoxy-2-[ 18 F]fluoro-D-glucose ([ 18 F]FDG). The scans showed that [ 68 Ga]Ga-DOTA-K-A9 accumulated in all the infected mice at injected doses ≥3.6 MBq. However, the tracer was not found to be selective towards infection, since the [ 68 Ga]Ga-DOTA-K-A9 also accumulated in mice with inflammation. In a concurrent in vitro binding evaluation performed with a 5-Carboxytetramethylrhodamine (TAMRA) fluorescence analogue of the peptide, TAMRA-K-A9, the microscopy results suggested that TAMRA-K-A9 bound to an intracellular epitope and therefore preferentially targeted dead bacteria. Thus, the [ 68 Ga]Ga-DOTA-K-A9 uptake observed in vivo is presumably a combination of local hyperaemia, vascular leakiness and/or binding to an epitope present in dead bacteria. This article is protected by copyright. All rights reserved.

The short-circuit test results of 6.9 kV/2.3 kV 400 kVA-class YBCO model transformer with fault current limiting function

International Nuclear Information System (INIS)

Tomioka, A.; Bohno, T.; Kakami, S.; Isozaki, M.; Watanabe, K.; Toyama, K.; Sugiyama, S.; Konno, M.; Gosho, Y.; Okamoto, H.; Hayashi, H.; Tsutsumi, T.; Iwakuma, M.; Saito, T.; Tanabe, K.; Shiohara, Y.

2013-01-01

Highlights: ► We manufactured the 400 kV A-class YBCO model transformer with FCL function. ► Short-circuit test was performed by applying 6.9 kV on primary side. ► The short-circuit current was limited to 174 A for a prospective current of 559 A. ► It agreed with the design and we also confirmed the I c did not degrade. ► The results suggest the possibility to design YBCO transformers with FCL function. -- Abstract: We are developing an elemental technology for 66/6.9 kV 20 MVA-class superconducting power transformer with fault current limiting function. In order to obtain the characteristics of YBCO conductor when the AC over current supplied to the conductor, the model coils were manufactured with YBCO tapes and tested. Based on these results, we manufactured the 6.9 kV/2.3 kV 400 kVA-class YBCO model transformer with fault current limiting function and performed short-circuit test. At the 0.25 s after short-circuit, the short-circuit current of primary winding was limited to about 174 A for a prospective current of 559 A. It was consistent with the design. The I–V characteristics of the winding did not change before and after the test. We consider the model transformer to be able to withstand AC over-current with the function of current limiting. The results suggest the possibility to design YBCO superconducting transformers with fault current limiting function for practical power grid

Joint Top-K Spatial Keyword Query Processing

DEFF Research Database (Denmark)

Wu, Dingming; Yiu, Man Lung; Cong, Gao

2012-01-01

Web users and content are increasingly being geopositioned, and increased focus is being given to serving local content in response to web queries. This development calls for spatial keyword queries that take into account both the locations and textual descriptions of content. We study the effici......Web users and content are increasingly being geopositioned, and increased focus is being given to serving local content in response to web queries. This development calls for spatial keyword queries that take into account both the locations and textual descriptions of content. We study...... the efficient, joint processing of multiple top-k spatial keyword queries. Such joint processing is attractive during high query loads and also occurs when multiple queries are used to obfuscate a user's true query. We propose a novel algorithm and index structure for the joint processing of top-k spatial...... keyword queries. Empirical studies show that the proposed solution is efficient on real data sets. We also offer analytical studies on synthetic data sets to demonstrate the efficiency of the proposed solution. Index Terms IEEE Terms Electronic mail , Google , Indexes , Joints , Mobile communication...

Test Results of the LARP HQ02b Magnet at 1.9 K

CERN Document Server

Bajas, H; Bottura, L; Chiuchiolo, A; Dunkel, O; Ferracin, P; Feuvrier, J; Giloux, Chr; Todesco, E; Ravaioli, E; Caspi, S; Dietderich, D; Felice, H; Hafalia, A R; Marchevsky, M; Sabbi, G L; Wang, X; Salmi, T; Ghosh, A; Schmalzle, J; Wanderer, P; Anerella, M; Ambrosio, G; Bossert, R; Chlachidze, G; Yu, M

2015-01-01

The HQ magnet is a 120 mm aperture, 1-meter-long Nb$_{3}$Sn quadrupole developed by the LARP collaboration in the framework of the High-Luminosity LHC project. A first series of coils was assembled and tested in 5 assemblies of the HQ01 series. The HQ01e model achieved a maximum gradient of 170 T/m at 4.5 K at LBNL in 2010-2011 and reached 184 T/m at 1.9 K at CERN in 2012. A new series of coils incorporating major design changes was fabricated for the HQ02 series. The first model, HQ02a, was tested at Fermilab where it reached 98% of the short sample limit at 4.5 K with a gradient of 182 T/m in 2013. However, the full training of the coils at 1.9 K could not be performed due to a current limit of 15 kA. Following this test, the azimuthal coil pre-load was increased by about 30 MPa and an additional current lead was installed at the electrical center of the magnet for quench protection studies. The test name of this magnet changed to HQ02b. In 2014, HQ02b was then shipped to CERN as the first opportunity for f...

Inhibition of enterovirus 71 replication by an α-hydroxy-nitrile derivative NK-1.9k.

Science.gov (United States)

Wang, Yaxin; Cao, Lin; Zhai, Yangyang; Ma, Jiaming; Nie, Quandeng; Li, Ting; Yin, Zheng; Sun, Yuna; Shang, Luqing

2017-05-01

Enterovirus 71 (EV71) is one of the major etiological agents of human hand-foot-and-mouth disease (HFMD) worldwide. EV71 infection in young children and people with immunodeficiency causes severe symptoms with a high fatality rates. However, there is still no approved drugs to treat such infections. Based on our previous report of a peptide-aldehyde anti-EV71 protease, we present here a highly specific α-hydroxy-nitrile derivative NK-1.9k, which inhibited the proliferation of multiple EV71 strains and coxsackievirus A16 (CVA16) in various cells with EC 50 of 37.0 nM with low cytotoxicity (CC 50  > 200 μM). The hydroxy-nitrile covalent warhead conferred NK-1.9k high potency and selectivity to interact with the cysteine residue of the active site of the viral protease. We also documented the resistance to NK-1.9k with a N69S mutation in EV71 3C pro . The combination of NK-1.9k and EV71 polymerase or entry inhibitors produced strong synergistic antiviral effects. Collectively, our findings suggest our compounds can potentially be developed as drugs for the treatment of HFMD. Copyright © 2017. Published by Elsevier B.V.

File list: His.PSC.20.H3K9ac.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available His.PSC.20.H3K9ac.AllCell mm9 Histone H3K9ac Pluripotent stem cell SRX185844,SRX185...848,SRX873351,SRX873349,SRX873345,SRX097641,SRX130021,SRX873347,SRX187620 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.H3K9ac.AllCell.bed ...

Epigenomic analysis in a cell-based model reveals the roles of H3K9me3 in breast cancer transformation.

Science.gov (United States)

Li, Qing-Lan; Lei, Pin-Ji; Zhao, Quan-Yi; Li, Lianyun; Wei, Gang; Wu, Min

2017-08-01

Epigenetic marks are critical regulators of chromatin and gene activity. Their roles in normal physiology and disease states, including cancer development, still remain elusive. Herein, the epigenomic change of H3K9me3, as well as its potential impacts on gene activity and genome stability, was investigated in an in vitro breast cancer transformation model. The global H3K9me3 level was studied with western blotting. The distribution of H3K9me3 on chromatin and gene expression was studied with ChIP-Seq and RNA-Seq, respectively. The global H3K9me3 level decreases during transformation and its distribution on chromatin is reprogrammed. By combining with TCGA data, we identified 67 candidate oncogenes, among which five genes are totally novel. Our analysis further links H3K9me3 with transposon activity, and suggests H3K9me3 reduction increases the cell's sensitivity to DNA damage reagents. H3K9me3 reduction is possibly related with breast cancer transformation by regulating gene expression and chromatin stability during transformation.

Backward K*/sup 0/ production in the reaction pi /sup -/p to Lambda /sup 0/K/sup +/ pi /sup -/ at 9 and 12 GeV/c

CERN Document Server

Ferrer, A; Bouquet, B; D'Almagne, B; De Rosny, G; Nguyen, H; Petroff, P; Richard, F; Rivet, P; Roudeau, P; Rougé, A; Six, J; Treille, D; Volte, A; Yoshida, H

1981-01-01

The authors have analysed about 85000 fast Lambda /sup 0/ events, obtained in a fast proton triggered experiment performed at the CERN- Omega spectrometer at 9 and 12 GeV/c incident pi /sup -/ beam. Nearly 2500 Lambda /sup 0/K/sup +/ pi /sup -/ events have been isolated. They find strong production of quasi-two-body processes Lambda /sup 0/K* /sup 0/ and Sigma */sup -/K/sup +/ consistent with u-channel hyperon exchange. Results on Lambda /sup 0/ polarization, K*/sup 0/ decay parameters and differential cross sections are given for Lambda /sup 0 /K*/sup 0/(892) and Lambda /sup 0/K*/sup 0/(1430) final states. A comparison is made with the associated backward Lambda /sup 0/(1520)K* /sup 0/ production seen in the four-prong reaction pi /sup -/p to pK /sup -/K/sup +/ pi /sup -/ obtained in the same experiment. (13 refs).

293 K - 1.9 K supporting systems for the Large Hadron Collider (LHC) cryo-magnets

CERN Document Server

Mathieu, M; Renaglia, T; Rohmig, P; Williams, L R

1998-01-01

The LHC machine will incorporate some 2000 main ring super-conducting magnets cooled at 1.9 K by super-fluid pressurized helium, mainly 15m-long dipoles with their cryostats and 6m-long quadrupoles housed in the Short Straight Section (SSS) units. This paper presents the design of the support system of the LHC arc cryo-magnets between 1.9 K at the cold mass and 293 K at the cryostat vacuum vessel. The stringent positioning precision for magnet alignment and the high thermal performance for cryogenic efficiency are the main conflicting requirements, which have lead to a trade-off design. The systems retained for LHC are based on column-type supports positioned in the vertical plane of the magnets inside the cryostats. An ad-hoc design has been achieved both for cryo-dipoles and SSS. Each column is composed of a main tubular thin-walled structure in composite material (glass-fibre/epoxy resin, for its low thermal conductivity properties), interfaced to both magnet and cryostat via stainless steel flanges. The t...

Data Quality Objectives Process for Designation of K-Basins Debris

International Nuclear Information System (INIS)

WESTCOTT, J.L.

2000-01-01

The U.S. Department of Energy has developed a schedule and approach for the removal of spent fuels, sludge, and debris from the K East (KE) and K West (KW) Basins, located in the 100 Area at the Hanford Site. The project that is the subject of this data quality objective (DQO) process is focused on the removal of debris from the K Basins and onsite disposal of the debris at the Environmental Restoration Disposal Facility (ERDF). This material previously has been dispositioned at the Hanford Low-Level Burial Grounds (LLBGs) or Central Waste Complex (CWC). The goal of this DQO process and the resulting Sampling and Analysis Plan (SAP) is to provide the strategy for characterizing and designating the K-Basin debris to determine if it meets the Environmental Restoration Disposal Facility Waste Acceptance Criteria (WAC), Revision 3 (BHI 1998). A critical part of the DQO process is to agree on regulatory and WAC interpretation, to support preparation of the DQO workbook and SAP

9-cis-retinoic Acid and troglitazone impacts cellular adhesion, proliferation, and integrin expression in K562 cells.

Science.gov (United States)

Hanson, Amanda M; Gambill, Jessica; Phomakay, Venusa; Staten, C Tyler; Kelley, Melissa D

2014-01-01

Retinoids are established pleiotropic regulators of both adaptive and innate immune responses. Recently, troglitazone, a PPAR gamma agonist, has been demonstrated to have anti-inflammatory effects. Separately, retinoids and troglitazone are implicated in immune related processes; however, their combinatory role in cellular adhesion and proliferation has not been well established. In this study, the effect of 9-cis-retinoic acid (9-cis-RA) and troglitazone on K562 cellular adhesion and proliferation was investigated. Troglitazone exposure decreased K562 cellular adhesion to RGD containing extracellular matrix proteins fibronectin, FN-120, and vitronectin in a concentration and time-dependent manner. In the presence of troglitazone, 9-cis-retinoic acid restores cellular adhesion to levels comparable to vehicle treatment alone on fibronectin, FN-120, and vitronectin substrates within 72 hours. Due to the prominent role of integrins in attachment to extracellular matrix proteins, we evaluated the level of integrin α5 subunit expression. Troglitazone treatment results in decrease in α5 subunit expression on the cell surface. In the presence of both agonists, cell surface α5 subunit expression was restored to levels comparable to vehicle treatment alone. Additionally, troglitazone and 9-cis-RA mediated cell adhesion was decreased in the presence of a function blocking integrin alpha 5 inhibitor. Further, through retinoid metabolic profiling and HPLC analysis, our study demonstrates that troglitazone augments retinoid availability in K562 cells. Finally, we demonstrate that troglitazone and 9-cis-retinoic acid synergistically dampen cellular proliferation in K562 cells. Our study is the first to report that the combination of troglitazone and 9-cis-retinoic acid restores cellular adhesion, alters retinoid availability, impacts integrin expression, and dampens cellular proliferation in K562 cells.

26 CFR 301.6103(k)(9)-1 - Disclosure of returns and return information relating to payment of tax by credit card and debit...

Science.gov (United States)

2010-04-01

... relating to payment of tax by credit card and debit card. 301.6103(k)(9)-1 Section 301.6103(k)(9)-1... Disclosure of returns and return information relating to payment of tax by credit card and debit card... processing credit card and debit card transactions to effectuate payment of tax as authorized by § 301.6311-2...

A histone H3K9M mutation traps histone methyltransferase Clr4 to prevent heterochromatin spreading

Energy Technology Data Exchange (ETDEWEB)

Shan, Chun-Min; Wang, Jiyong; Xu, Ke; Chen, Huijie; Yue, Jia-Xing; Andrews, Stuart; Moresco, James J.; Yates, John R.; Nagy, Peter L.; Tong, Liang; Jia, Songtao

2016-09-20

Histone lysine-to-methionine (K-to-M) mutations are associated with multiple cancers, and they function in a dominant fashion to block the methylation of corresponding lysines on wild type histones. However, their mechanisms of function are controversial. Here we show that in fission yeast, introducing the K9M mutation into one of the three histone H3 genes dominantly blocks H3K9 methylation on wild type H3 across the genome. In addition, H3K9M enhances the interaction of histone H3 tail with the H3K9 methyltransferase Clr4 in a SAM (S-adenosyl-methionine)-dependent manner, and Clr4 is trapped at nucleation sites to prevent its spreading and the formation of large heterochromatin domains. We further determined the crystal structure of an H3K9M peptide in complex with human H3K9 methyltransferase G9a and SAM, which reveales that the methionine side chain had enhanced van der Waals interactions with G9a. Therefore, our results provide a detailed mechanism by which H3K9M regulates H3K9 methylation.

Effects on atmospherics at 6 kHz and 9 kHz recorded at Tripura during the India-Pakistan Border earthquake

Directory of Open Access Journals (Sweden)

S. S. De

2010-04-01

Full Text Available The outcome of the results of some analyses of electromagnetic emissions recorded by VLF receivers at 6 kHz and 9 kHz over Agartala, Tripura, the North-Eastern state of India (Lat. 23° N, Long. 91.4° E during the large earthquake at Muzaffarabad (Lat. 34.53° N, Long. 73.58° E at Kashmir under Pakistan have been presented here. Spiky variations in integrated field intensity of atmospherics (IFIA at 6 and 9 kHz have been observed 10 days prior (from midnight of 28 September 2005 to the day of occurrence of the earthquake on 8 October 2005 and the effect continued, decayed gradually and eventually ceased on 16 October 2005. The spikes distinctly superimposed on the ambient level with mutual separation of 2–5 min. Occurrence number of spikes per hour and total duration of their occurrence have been found remarkably high on the day of occurrence of the earthquake. The spike heights are higher at 6 kHz than at 9 kHz. The results have been explained on the basis of generation of electromagnetic radiation associated with fracture of rocks, their subsequent penetration into the Earth's atmosphere and finally their propagation between Earth-ionosphere waveguide. The present observation shows that VLF anomaly is well-confined between 6 and 9 kHz.

Hydrophobic core substitutions in calbindin D9k

DEFF Research Database (Denmark)

Kragelund, B B; Jönsson, M; Bifulco, G

1998-01-01

Hydrophobic core residues have a marked influence on the Ca2+-binding properties of calbindin D9k, even though there are no direct contacts between these residues and the bound Ca2+ ions. Eleven different mutants with substitutions in the hydrophobic core were produced, and their equilibrium Ca2+...... that the hydrophobic core residues promote Ca2+ binding both by contributing to the preformation of the Ca2+ sites in the apo state and by preferentially stabilizing the Ca2+-bound state.......Hydrophobic core residues have a marked influence on the Ca2+-binding properties of calbindin D9k, even though there are no direct contacts between these residues and the bound Ca2+ ions. Eleven different mutants with substitutions in the hydrophobic core were produced, and their equilibrium Ca2...... that the mutation causes only very minimal perturbations in the immediate vicinity of residue 61. Substitutions of alanines or glycines for bulky residues in the center of the core were found to have significant effects on both Ca2+ affinity and dissociation rates. These substitutions caused a reduction in affinity...

Test Results of the LARP HQ02b Magnet at 1.9 K

OpenAIRE

Bajas, H; Bajko, M; Bottura, L; Chiuchiolo, A; Dunkel, O; Ferracin, P; Feuvrier, J; Giloux, Chr; Todesco, E; Ravaioli, E; Caspi, S; Dietderich, D; Felice, H; Hafalia, A R; Marchevsky, M

2015-01-01

The HQ magnet is a 120 mm aperture, 1-meter-long Nb3Sn quadrupole developed by the LARP collaboration in the framework of the High-Luminosity LHC project. A first series of coils was assembled and tested in 5 assemblies of the HQ01 series. The HQ01e model achieved a maximum gradient of 170 T/m at 4.5 K at LBNL in 2010-2011 and reached 184 T/m at 1.9 K at CERN in 2012. A new series of coils incorporating major design changes was fabricated for the HQ02 series. The first model, HQ02a, was teste...

Delayed wound healing in aged skin rat models after thermal injury is associated with an increased MMP-9, K6 and CD44 expression.

Science.gov (United States)

Simonetti, Oriana; Oriana, Simonetti; Lucarini, Guendalina; Guendalina, Lucarini; Cirioni, Oscar; Oscar, Cirioni; Zizzi, Antonio; Antonio, Zizzi; Orlando, Fiorenza; Fiorenza, Orlando; Provinciali, Mauro; Mauro, Provinciali; Di Primio, Roberto; Roberto, Di Primio; Giacometti, Andrea; Andrea, Giacometti; Offidani, Annamaria; Annamaria, Offidani

2013-06-01

Age-related differences in wound healing have been documented but little is known about the wound healing mechanism after burns. Our aim was to compare histological features and immunohistochemical expression of matrix metalloproteinase-9 (MMP-9), collagen IV, K6 and CD44 in the burn wound healing process in aged and young rats. Following burns the appearance of the wound bed in aged rats had progressed but slowly, resulting in a delayed healing process compared to the young rats. At 21 days after injury, epithelial K6, MMP-9 and CD44 expression was significantly increased in aged rats with respect to young rats; moreover, in the aged rat group we observed a not fully reconstituted basement membrane. K6, MMP-9 and CD44 expression was significantly increased in wounded skin compared to unwounded skin both in young and aged rats. We hypothesise that delayed burn skin wound healing process in the aged rats may represent an age dependent response to injury where K6, MMP-9 and CD44 play a key role. It is therefore possible to suggest that these factors contribute to the delayed wound healing in aged skin and that modulation could lead to a better and faster recovery of skin damage in elderly. Copyright © 2012 Elsevier Ltd and ISBI. All rights reserved.

9 Boo is a K-giant with high abundance of lithium

International Nuclear Information System (INIS)

Khyanni, L.

1984-01-01

An unusually strong lithium resonance lipe lambda 6707.8 was detected in the spectrum of the K-giant 9 Boo. The lithium abundance lg Nsub(Li)=2.5+-0.5 is estimated from a theoretical curve of growth calculated for a model atmosphere with Tsub(eff)=4000 K, lg g=2.0

Continual removal of H3K9 promoter methylation by Jmjd2 demethylases is vital for ESC self-renewal and early development

NARCIS (Netherlands)

Pedersen, Marianne Terndrup; Kooistra, Susanne Marije; Radzisheuskaya, Aliaksandra; Laugesen, Anne; Johansen, Jens Vilstrup; Hayward, Daniel Geoffrey; Nilsson, Jakob; Agger, Karl; Helin, Kristian

2016-01-01

Chromatin-associated proteins are essential for the specification and maintenance of cell identity. They exert these functions through modulating and maintaining transcriptional patterns. To elucidate the functions of the Jmjd2 family of H3K9/H3K36 histone demethylases, we generated conditional

55K isoform of CDK9 associates with Ku70 and is involved in DNA repair

International Nuclear Information System (INIS)

Liu, Hongbing; Herrmann, Christine H.; Chiang, Karen; Sung, Tzu-Ling; Moon, Sung-Hwan; Donehower, Lawrence A.; Rice, Andrew P.

2010-01-01

Positive elongation factor b (P-TEFb) is a cellular protein kinase that is required for RNA polymerase II (RNAP II) transcriptional elongation of protein coding genes. P-TEFb is a set of different molecular complexes, each containing CDK9 as the catalytic subunit. There are two isoforms of the CDK9 protein - the major 42 KDa CDK9 isoform and the minor 55KDa isoform that is translated from an in-frame mRNA that arises from an upstream transcriptional start site. We found that shRNA depletion of the 55K CDK9 protein in HeLa cells induces apoptosis and double-strand DNA breaks (DSBs). The levels of apoptosis and DSBs induced by the depletion were reduced by expression of a 55K CDK9 protein variant resistant to the shRNA, indicating that these phenotypes are the consequence of depletion of the 55K protein and not off-target effects. We also found that the 55K CDK9 protein, but not the 42K CDK9 protein, specifically associates with Ku70, a protein involved in DSB repair. Our findings suggest that the 55K CDK9 protein may function in repair of DNA through an association with Ku70.

Characteristics of the process K+p→K+anti ppp at 12 GeV/c

International Nuclear Information System (INIS)

Armstrong, T.A.; Frame, D.; Hughes, I.S.; Kumar, B.R.; Lewis, G.M.; Macallister, J.B.; Stewart, D.T.; Turnbull, R.M.

1979-01-01

Data are presented on the reaction K + p→K + anti ppp at 12 GeV/c from an experiment using the OMEGA spectrometer at CERN. A clear Λ(1520) signal is observed in the process K + p→anti Λ(1520)pp and angular distributions and correlations are presented for this process. The angular distributions for the reaction in which anti Λ(1520) is not produced show an appreciable backward K + peak. (Auth.)

ERRα induces H3K9 demethylation by LSD1 to promote cell invasion

Science.gov (United States)

Carnesecchi, Julie; Forcet, Christelle; Zhang, Ling; Tribollet, Violaine; Barenton, Bruno; Boudra, Rafik; Cerutti, Catherine; Billas, Isabelle M. L.; Sérandour, Aurélien A.; Carroll, Jason S.; Beaudoin, Claude; Vanacker, Jean-Marc

2017-01-01

Lysine Specific Demethylase 1 (LSD1) removes mono- and dimethyl groups from lysine 4 of histone H3 (H3K4) or H3K9, resulting in repressive or activating (respectively) transcriptional histone marks. The mechanisms that control the balance between these two antagonist activities are not understood. We here show that LSD1 and the orphan nuclear receptor estrogen-related receptor α (ERRα) display commonly activated genes. Transcriptional activation by LSD1 and ERRα involves H3K9 demethylation at the transcriptional start site (TSS). Strikingly, ERRα is sufficient to induce LSD1 to demethylate H3K9 in vitro. The relevance of this mechanism is highlighted by functional data. LSD1 and ERRα coregulate several target genes involved in cell migration, including the MMP1 matrix metallo-protease, also activated through H3K9 demethylation at the TSS. Depletion of LSD1 or ERRα reduces the cellular capacity to invade the extracellular matrix, a phenomenon that is rescued by MMP1 reexpression. Altogether our results identify a regulatory network involving a direct switch in the biochemical activities of a histone demethylase, leading to increased cell invasion. PMID:28348226

ERRα induces H3K9 demethylation by LSD1 to promote cell invasion.

Science.gov (United States)

Carnesecchi, Julie; Forcet, Christelle; Zhang, Ling; Tribollet, Violaine; Barenton, Bruno; Boudra, Rafik; Cerutti, Catherine; Billas, Isabelle M L; Sérandour, Aurélien A; Carroll, Jason S; Beaudoin, Claude; Vanacker, Jean-Marc

2017-04-11

Lysine Specific Demethylase 1 (LSD1) removes mono- and dimethyl groups from lysine 4 of histone H3 (H3K4) or H3K9, resulting in repressive or activating (respectively) transcriptional histone marks. The mechanisms that control the balance between these two antagonist activities are not understood. We here show that LSD1 and the orphan nuclear receptor estrogen-related receptor α (ERRα) display commonly activated genes. Transcriptional activation by LSD1 and ERRα involves H3K9 demethylation at the transcriptional start site (TSS). Strikingly, ERRα is sufficient to induce LSD1 to demethylate H3K9 in vitro. The relevance of this mechanism is highlighted by functional data. LSD1 and ERRα coregulate several target genes involved in cell migration, including the MMP1 matrix metallo-protease, also activated through H3K9 demethylation at the TSS. Depletion of LSD1 or ERRα reduces the cellular capacity to invade the extracellular matrix, a phenomenon that is rescued by MMP1 reexpression. Altogether our results identify a regulatory network involving a direct switch in the biochemical activities of a histone demethylase, leading to increased cell invasion.

Design and development of solid carbide step drill K34 for machining of CFRP and GFRP composite laminates

Science.gov (United States)

Rangaswamy, T.; Nagaraja, R.

2018-04-01

The Study focused on design and development of solid carbide step drill K34 to drill holes on composite materials such as Carbon Fiber Reinforced Plastic (CFRP) and Glass Fiber Reinforced Plastic (GFRP). The step drill K34 replaces step wise drilling of diameter 6.5mm and 9 mm holes that reduces the setup time, cutting speed, feed rate cost, delamination and increase the production rate. Several researchers have analyzed the effect of drilling process on various fiber reinforced plastic composites by carrying out using conventional tools and machinery. However, this process operation can lead to different kind of damages such as delamination, fiber pullout, and local cracks. To avoid the problems encountered at the time of drilling, suitable tool material and geometry is essential. This paper deals with the design and development of K34 Carbide step drill used to drill holes on CFRP and GFRP laminates. An Experimental study carried out to investigate the tool geometry, feed rate and cutting speed that avoids delamination and fiber breakage.

Defense Waste Processing Facility Simulant Chemical Processing Cell Studies for Sludge Batch 9

International Nuclear Information System (INIS)

Smith, Tara E.; Newell, J. David; Woodham, Wesley H.

2016-01-01

The Savannah River National Laboratory (SRNL) received a technical task request from Defense Waste Processing Facility (DWPF) and Saltstone Engineering to perform simulant tests to support the qualification of Sludge Batch 9 (SB9) and to develop the flowsheet for SB9 in the DWPF. These efforts pertained to the DWPF Chemical Process Cell (CPC). CPC experiments were performed using SB9 simulant (SB9A) to qualify SB9 for sludge-only and coupled processing using the nitric-formic flowsheet in the DWPF. Two simulant batches were prepared, one representing SB8 Tank 40H and another representing SB9 Tank 51H. The simulant used for SB9 qualification testing was prepared by blending the SB8 Tank 40H and SB9 Tank 51H simulants. The blended simulant is referred to as SB9A. Eleven CPC experiments were run with an acid stoichiometry ranging between 105% and 145% of the Koopman minimum acid equation (KMA), which is equivalent to 109.7% and 151.5% of the Hsu minimum acid factor. Three runs were performed in the 1L laboratory scale setup, whereas the remainder were in the 4L laboratory scale setup. Sludge Receipt and Adjustment Tank (SRAT) and Slurry Mix Evaporator (SME) cycles were performed on nine of the eleven. The other two were SRAT cycles only. One coupled flowsheet and one extended run were performed for SRAT and SME processing. Samples of the condensate, sludge, and off-gas were taken to monitor the chemistry of the CPC experiments.

Defense Waste Processing Facility Simulant Chemical Processing Cell Studies for Sludge Batch 9

Energy Technology Data Exchange (ETDEWEB)

Smith, Tara E. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Newell, J. David [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Woodham, Wesley H. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

2016-08-10

The Savannah River National Laboratory (SRNL) received a technical task request from Defense Waste Processing Facility (DWPF) and Saltstone Engineering to perform simulant tests to support the qualification of Sludge Batch 9 (SB9) and to develop the flowsheet for SB9 in the DWPF. These efforts pertained to the DWPF Chemical Process Cell (CPC). CPC experiments were performed using SB9 simulant (SB9A) to qualify SB9 for sludge-only and coupled processing using the nitric-formic flowsheet in the DWPF. Two simulant batches were prepared, one representing SB8 Tank 40H and another representing SB9 Tank 51H. The simulant used for SB9 qualification testing was prepared by blending the SB8 Tank 40H and SB9 Tank 51H simulants. The blended simulant is referred to as SB9A. Eleven CPC experiments were run with an acid stoichiometry ranging between 105% and 145% of the Koopman minimum acid equation (KMA), which is equivalent to 109.7% and 151.5% of the Hsu minimum acid factor. Three runs were performed in the 1L laboratory scale setup, whereas the remainder were in the 4L laboratory scale setup. Sludge Receipt and Adjustment Tank (SRAT) and Slurry Mix Evaporator (SME) cycles were performed on nine of the eleven. The other two were SRAT cycles only. One coupled flowsheet and one extended run were performed for SRAT and SME processing. Samples of the condensate, sludge, and off-gas were taken to monitor the chemistry of the CPC experiments.

Inhibition of the H3K9 methyltransferase G9A attenuates oncogenicity and activates the hypoxia signaling pathway.

Directory of Open Access Journals (Sweden)

Jolene Caifeng Ho

Full Text Available Epigenetic mechanisms play important roles in the regulation of tumorigenesis, and hypoxia-induced epigenetic changes may be critical for the adaptation of cancer cells to the hypoxic microenvironment of solid tumors. Previously, we showed that loss-of-function of the hypoxia-regulated H3K9 methyltransferase G9A attenuates tumor growth. However, the mechanisms by which blockade of G9A leads to a tumor suppressive effect remain poorly understood. We show that G9A is highly expressed in breast cancer and is associated with poor patient prognosis, where it may function as a potent oncogenic driver. In agreement with this, G9A inhibition by the small molecule inhibitor, BIX-01294, leads to increased cell death and impaired cell migration, cell cycle and anchorage-independent growth. Interestingly, whole transcriptome analysis revealed that genes involved in diverse cancer cell functions become hypoxia-responsive upon G9A inhibition. This was accompanied by the upregulation of the hypoxia inducible factors HIF1α and HIF2α during BIX-01294 treatment even in normoxia that may facilitate the tumor suppressive effects of BIX-01294. HIF inhibition was able to reverse some of the transcriptional changes induced by BIX-01294 in hypoxia, indicating that the HIFs may be important drivers of these derepressed target genes. Therefore, we show that G9A is a key mediator of oncogenic processes in breast cancer cells and G9A inhibition by BIX-01294 can successfully attenuate oncogenicity even in hypoxia.

Inhibition of the H3K9 methyltransferase G9A attenuates oncogenicity and activates the hypoxia signaling pathway

Science.gov (United States)

Ho, Jolene Caifeng; Abdullah, Lissa Nurrul; Pang, Qing You; Jha, Sudhakar; Chow, Edward Kai-Hua; Yang, Henry; Kato, Hiroyuki; Ueda, Jun

2017-01-01

Epigenetic mechanisms play important roles in the regulation of tumorigenesis, and hypoxia-induced epigenetic changes may be critical for the adaptation of cancer cells to the hypoxic microenvironment of solid tumors. Previously, we showed that loss-of-function of the hypoxia-regulated H3K9 methyltransferase G9A attenuates tumor growth. However, the mechanisms by which blockade of G9A leads to a tumor suppressive effect remain poorly understood. We show that G9A is highly expressed in breast cancer and is associated with poor patient prognosis, where it may function as a potent oncogenic driver. In agreement with this, G9A inhibition by the small molecule inhibitor, BIX-01294, leads to increased cell death and impaired cell migration, cell cycle and anchorage-independent growth. Interestingly, whole transcriptome analysis revealed that genes involved in diverse cancer cell functions become hypoxia-responsive upon G9A inhibition. This was accompanied by the upregulation of the hypoxia inducible factors HIF1α and HIF2α during BIX-01294 treatment even in normoxia that may facilitate the tumor suppressive effects of BIX-01294. HIF inhibition was able to reverse some of the transcriptional changes induced by BIX-01294 in hypoxia, indicating that the HIFs may be important drivers of these derepressed target genes. Therefore, we show that G9A is a key mediator of oncogenic processes in breast cancer cells and G9A inhibition by BIX-01294 can successfully attenuate oncogenicity even in hypoxia. PMID:29145444

Transcriptional repression is epigenetically marked by H3K9 methylation during SV40 replication

OpenAIRE

Kallestad, Les; Christensen, Kendra; Woods, Emily; Milavetz, Barry

2014-01-01

Background We have recently shown that T-antigen binding to Site I results in the replication-dependent introduction of H3K9me1 into SV40 chromatin late in infection. Since H3K9me2 and H3K9me3 are also present late in infection, we determined whether their presence was also related to the status of ongoing transcription and replication. Transcription was either inhibited with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidizole (DRB) or stimulated with sodium butyrate and the effects on histone m...

Distinct pharmacology and metabolism of K2 synthetic cannabinoids compared to Δ9-THC: Mechanism underlying greater toxicity?

Science.gov (United States)

Fantegrossi, William E.; Moran, Jeffery H.; Radominska-Pandya, Anna; Prather, Paul L.

2013-01-01

K2 or Spice products are emerging drugs of abuse that contain synthetic cannabinoids (SCBs). Although assumed by many teens and first time drug users to be a “safe” and “legal” alternative to marijuana, many recent reports indicate that SCBs present in K2 produce toxicity not associated with the primary psychoactive component of marijuana, Δ9-tetrahydrocannabinol (Δ9-THC). This mini-review will summarize recent evidence that use of K2 products poses greater health risks relative to marijuana, and suggest that distinct pharmacological properties and metabolism of SCBs relative to Δ9-THC may contribute to the observed toxicity. Studies reviewed will indicate that in contrast to partial agonist properties of Δ9-THC typically observed in vitro, SCBs in K2 products act as full cannabinoid receptor type 1 (CB1R) and type 2 (CB2R) agonists in both cellular assays and animal studies. Furthermore, unlike Δ9-THC metabolism, several SCB metabolites retain high affinity for, and exhibit a range of intrinsic activities at, CB1 and CB2Rs. Finally, several reports indicate that although quasi-legal SCBs initially evaded detection and legal consequences, these presumed “advantages” have been limited by new legislation and development of product and human testing capabilities. Collectively, evidence reported in this mini-review suggests that K2 products are neither safe nor legal alternatives to marijuana. Instead, enhanced toxicity of K2 products relative to marijuana, perhaps resulting from the combined actions of a complex mixture of different SCBs present and their active metabolites that retain high affinity for CB1 and CB2Rs, highlights the inherent danger that may accompany use of these substances. PMID:24084047

Distinct pharmacology and metabolism of K2 synthetic cannabinoids compared to Δ(9)-THC: mechanism underlying greater toxicity?

Science.gov (United States)

Fantegrossi, William E; Moran, Jeffery H; Radominska-Pandya, Anna; Prather, Paul L

2014-02-27

K2 or Spice products are emerging drugs of abuse that contain synthetic cannabinoids (SCBs). Although assumed by many teens and first time drug users to be a "safe" and "legal" alternative to marijuana, many recent reports indicate that SCBs present in K2 produce toxicity not associated with the primary psychoactive component of marijuana, ∆(9)-tetrahydrocannabinol (Δ(9)-THC). This mini-review will summarize recent evidence that use of K2 products poses greater health risks relative to marijuana, and suggest that distinct pharmacological properties and metabolism of SCBs relative to Δ(9)-THC may contribute to the observed toxicity. Studies reviewed will indicate that in contrast to partial agonist properties of Δ(9)-THC typically observed in vitro, SCBs in K2 products act as full cannabinoid receptor type 1 (CB1R) and type 2 (CB2R) agonists in both cellular assays and animal studies. Furthermore, unlike Δ(9)-THC metabolism, several SCB metabolites retain high affinity for, and exhibit a range of intrinsic activities at, CB1 and CB2Rs. Finally, several reports indicate that although quasi-legal SCBs initially evaded detection and legal consequences, these presumed "advantages" have been limited by new legislation and development of product and human testing capabilities. Collectively, evidence reported in this mini-review suggests that K2 products are neither safe nor legal alternatives to marijuana. Instead, enhanced toxicity of K2 products relative to marijuana, perhaps resulting from the combined actions of a complex mixture of different SCBs present and their active metabolites that retain high affinity for CB1 and CB2Rs, highlights the inherent danger that may accompany use of these substances. © 2013.

Development of K-Nearest Neighbour Regression Method in Forecasting River Stream Flow

Directory of Open Access Journals (Sweden)

Mohammad Azmi

2012-07-01

Full Text Available Different statistical, non-statistical and black-box methods have been used in forecasting processes. Among statistical methods, K-nearest neighbour non-parametric regression method (K-NN due to its natural simplicity and mathematical base is one of the recommended methods for forecasting processes. In this study, K-NN method is explained completely. Besides, development and improvement approaches such as best neighbour estimation, data transformation functions, distance functions and proposed extrapolation method are described. K-NN method in company with its development approaches is used in streamflow forecasting of Zayandeh-Rud Dam upper basin. Comparing between final results of classic K-NN method and modified K-NN (number of neighbour 5, transformation function of Range Scaling, distance function of Mahanalobis and proposed extrapolation method shows that modified K-NN in criteria of goodness of fit, root mean square error, percentage of volume of error and correlation has had performance improvement 45% , 59% and 17% respectively. These results approve necessity of applying mentioned approaches to derive more accurate forecasts.

Vitamin K2 biosynthetic enzyme, UBIAD1 is essential for embryonic development of mice.

Science.gov (United States)

Nakagawa, Kimie; Sawada, Natsumi; Hirota, Yoshihisa; Uchino, Yuri; Suhara, Yoshitomo; Hasegawa, Tomoka; Amizuka, Norio; Okamoto, Tadashi; Tsugawa, Naoko; Kamao, Maya; Funahashi, Nobuaki; Okano, Toshio

2014-01-01

UbiA prenyltransferase domain containing 1 (UBIAD1) is a novel vitamin K2 biosynthetic enzyme screened and identified from the human genome database. UBIAD1 has recently been shown to catalyse the biosynthesis of Coenzyme Q10 (CoQ10) in zebrafish and human cells. To investigate the function of UBIAD1 in vivo, we attempted to generate mice lacking Ubiad1, a homolog of human UBIAD1, by gene targeting. Ubiad1-deficient (Ubiad1(-/-)) mouse embryos failed to survive beyond embryonic day 7.5, exhibiting small-sized body and gastrulation arrest. Ubiad1(-/-) embryonic stem (ES) cells failed to synthesize vitamin K2 but were able to synthesize CoQ9, similar to wild-type ES cells. Ubiad1(+/-) mice developed normally, exhibiting normal growth and fertility. Vitamin K2 tissue levels and synthesis activity were approximately half of those in the wild-type, whereas CoQ9 tissue levels and synthesis activity were similar to those in the wild-type. Similarly, UBIAD1 expression and vitamin K2 synthesis activity of mouse embryonic fibroblasts prepared from Ubiad1(+/-) E15.5 embryos were approximately half of those in the wild-type, whereas CoQ9 levels and synthesis activity were similar to those in the wild-type. Ubiad1(-/-) mouse embryos failed to be rescued, but their embryonic lifespans were extended to term by oral administration of MK-4 or CoQ10 to pregnant Ubiad1(+/-) mice. These results suggest that UBIAD1 is responsible for vitamin K2 synthesis but may not be responsible for CoQ9 synthesis in mice. We propose that UBIAD1 plays a pivotal role in embryonic development by synthesizing vitamin K2, but may have additional functions beyond the biosynthesis of vitamin K2.

Description of the ππ → ππ, ππ → K anti K and K anti K → K anti K process in the framework of the N/D method

International Nuclear Information System (INIS)

Khenner, V.K.

1978-01-01

On the basis of the dispersion model the ππ → ππ, ππ → K anti K and K anti K → K anti K processes in the state with I=J=.0 are studied. The processes are considered using the N/D matrix method, permitting to solve precisely the related system of dispersion ratios for the partial amplitudes. It is shown that the discovery of the inelastic channel can be effectively taken into account by the introduction of the CDD (Castillejo-Dality-Dyson) term into the elastic scattering amplitude. It is established, that at a given ππ → ππ amplitude the transition from the complete multichannel problem, considered by the matrix method, to the linear problem leads to the solution, where all arbitrary constants are fixed in the case when zeros of the ππ → K anti K and ππ → ππ amplitudes coincide. In this case the ππ → K anti K amplitude coincides with that obtained by the Omnes-Muskhelishvili method. Other processes, where the interaction in the initial or final state is insignificant, can be also studied by such a method not resulting in the appearance of additional arbitrary parameters. The consideration of the problem of a resonance position in various channels has shown, that the matrix method does not obligatorily result in the appearance of the resonance with the permitted quantum numbers in all the interconnected channels

Expression of recombinant myostatin propeptide pPIC9K-Msp plasmid in Pichia pastoris.

Science.gov (United States)

Du, W; Xia, J; Zhang, Y; Liu, M J; Li, H B; Yan, X M; Zhang, J S; Li, N; Zhou, Z Y; Xie, W Z

2015-12-28

Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and the enhanced biological activity of myostatin propeptide mutant. Modified myostatin propeptide gene was cloned into the pPIC9K plasmid to form the recombinant plasmid pPIC9K-Msp. Recombinant plasmid pPIC9K-Msp was transformed into Pichia pastoris GS115 by electrotransformation. Transformed cells were screened, and methanol was used to induce expression. SDS-PAGE and western blotting were used to verify the successful expression of myostatin propeptide with biological activity in Pichia pastoris, providing the basis for characterization of this protein.

Long-term test of the 22.9kV HTS power cable system in LS Cable Ltd

International Nuclear Information System (INIS)

Jang, Hyun Man; Lee, Chang Young; Kim, Choon Dong; Kim, Do Hyung; Park, In Son; Ji, Bong Ki; Kim, Dong Wook; Cho, Jeonwook

2006-01-01

Since 2001, LS cable Ltd. has been developing the design, manufacturing and evaluation technologies for high temperature superconducting (HTS) power cable system as a member of DAPAS (Dream for Advanced Power system by Applied Superconductivity technology) program in Korea. The 30 m HTS cable system that is rated at 22.9 kV and 1.2 kA giving a rated capacity of 50 MVA had been developed and tested. The cable was designed as a cold dielectric type employing Bi-2223 HTS tapes and polypropylene (PP) laminated paper as the conductor and electrical insulation, respectively. The cable is cooled with sub-cooled liquid nitrogen at temperature from 75 to 77 K. The manufacturing and the installation of the cable system were completed in 2004. Long-term performance test of the cable system has been conducted for six months to verify its electric and mechanical properties in 2005

The CANDU 9 distributed control system design process

International Nuclear Information System (INIS)

Harber, J.E.; Kattan, M.K.; Macbeth, M.J.

1997-01-01

Canadian designed CANDU pressurized heavy water nuclear reactors have been world leaders in electrical power generation. The CANDU 9 project is AECL's next reactor design. Plant control for the CANDU 9 station design is performed by a distributed control system (DCS) as compared to centralized control computers, analog control devices and relay logic used in previous CANDU designs. The selection of a DCS as the platform to perform the process control functions and most of the data acquisition of the plant, is consistent with the evolutionary nature of the CANDU technology. The control strategies for the DCS control programs are based on previous CANDU designs but are implemented on a new hardware platform taking advantage of advances in computer technology. This paper describes the design process for developing the CANDU 9 DCS. Various design activities, prototyping and analyses have been undertaken in order to ensure a safe, functional, and cost-effective design. (author)

Changing Curriculum: A Critical Inquiry into the Revision of the British Columbia Science Curriculum For Grades K-9

Science.gov (United States)

Searchfield, Mary A.

In 2010 British Columbia's Ministry of Education started the process of redesigning the provincial school curriculum, Kindergarten to Grade 12. Mandatory implementation of the new curriculum was set for the 2016/17 school year for Grades K-9, and 2017/18 for Grades 10-12. With a concerted emphasis on personalized learning and through the frame of a Know-Do-Understand curriculum model, the new curriculum aims to meet the needs of today's learners, described as living in a technology-rich, fast-paced and ever-changing world, through a concept-based and competency-driven emphasis. This thesis is a critical analysis of the BC K-9 Science curriculum as written and published, looking specifically at how science is treated as a form of knowledge, its claimed presentation as a story, and on whether the intentions claimed by the designers are matched in the curriculum's final form.

K2-232 b: a transiting warm Saturn on an eccentric P = 11.2 d orbit around a V = 9.9 star

Science.gov (United States)

Brahm, R.; Espinoza, N.; Jordán, A.; Rojas, F.; Sarkis, P.; Díaz, M. R.; Rabus, M.; Drass, H.; Lachaume, R.; Soto, M. G.; Jenkins, J. S.; Jones, M. I.; Henning, Th; Pantoja, B.; Vučković, M.

2018-06-01

We report the discovery of K2-232 b using photometric data of the Kepler K2 satellite coupled with ground-based spectroscopic observations. K2-232 b has a mass of MP = 0.397 ± 0.037 MJ, a radius of RP = 1.00 ± 0.020 RJ, and a moderately low equilibrium temperature of Teq = 1030 ± 15 K due to its relatively large star-planet separation of a = 0.1036 au. K2-232 b orbits its bright (V = 9.9) late F-type host star in an eccentric orbit (e = 0.258 ± 0.025) every 11.2 d, and is one of only four well-characterized warm Jupiters having host stars brighter than V = 10. We estimate a heavy element content of 20 ± 7 M⊕ for K2-232 b, which is consistent with standard models of giant planet formation. The bright host star of K2-232 b makes this system a well-suited target for detailed follow-up observations that will aid in the study of the atmospheres and orbital evolution of giant planets at moderate separations from their host stars.

Kimberlite Wall Rock Fragmentation: Venetia K08 Pipe Development

Science.gov (United States)

Barnett, W.; Kurszlaukis, S.; Tait, M.; Dirks, P.

2009-05-01

Volcanic systems impose powerful disrupting forces on the country rock into which they intrude. The nature of the induced brittle deformation or fragmentation can be characteristic of the volcanic processes ongoing within the volcanic system, but are most typically partially removed or obscured by repeated, overprinting volcanic activity in mature pipes. Incompletely evolved pipes may therefore provide important evidence for the types and stages of wall rock fragmentation, and mechanical processes responsible for the fragmentation. Evidence for preserved stages of fragmentation is presented from a detailed study of the K08 pipe within the Cambrian Venetia kimberlite cluster, South Africa. This paper investigates the growth history of the K08 pipe and the mechanics of pipe development based on observations in the pit, drill core and thin sections, from geochemical analyses, particle size distribution analyses, and 3D modeling. Present open pit exposures of the K08 pipe comprise greater than 90% mega-breccia of country rock clasts (gneiss and schist) with Drill core shows that below about 225 m the CRB includes increasing quantities of kimberlite. The breccia clasts are angular, clast-supported with void or carbonate cement between the clasts. Average clast sizes define sub-horizontal layers tens of metres thick across the pipe. Structural and textural observations indicate the presence of zones of re-fragmentation or zones of brittle shearing. Breccia textural studies and fractal statistics on particle size distributions (PSD) is used to quantify sheared and non- sheared breccia zones. The calculated energy required to form the non-sheared breccia PSD implies an explosive early stage of fragmentation that pre-conditions the rock mass. The pre-conditioning would have been caused by explosions that are either phreatic or phreatomagmatic in nature. The explosions are likely to have been centered on a dyke, or pulses of preceding volatile-fluid phases, which have

Development and Evaluation of a Barley 50k iSelect SNP Array

Directory of Open Access Journals (Sweden)

Micha M. Bayer

2017-10-01

Full Text Available High-throughput genotyping arrays continue to be an attractive, cost-effective alternative to sequencing based approaches. We have developed a new 50k Illumina Infinium iSelect genotyping array for barley, a cereal crop species of major international importance. The majority of SNPs on the array have been extracted from variants called in exome capture data of a wide range of European barley germplasm. We used the recently published barley pseudomolecule assembly to map the exome capture data, which allowed us to generate markers with accurate physical positions and detailed gene annotation. Markers from an existing and widely used barley 9k Infinium iSelect array were carried over onto the 50k chip for backward compatibility. The array design featured 49,267 SNP markers that converted into 44,040 working assays, of which 43,461 were scorable in GenomeStudio. Of the working assays, 6,251 are from the 9k iSelect platform. We validated the SNPs by comparing the genotype calls from the new array to legacy datasets. Rates of agreement averaged 98.1 and 93.9% respectively for the legacy 9k iSelect SNP set (Comadran et al., 2012 and the exome capture SNPs. To test the utility of the 50k chip for genetic mapping, we genotyped a segregating population derived from a Golden Promise × Morex cross (Liu et al., 2014 and mapped over 14,000 SNPs to genetic positions which showed a near exact correspondence to their known physical positions. Manual adjustment of the cluster files used by the interpreting software for genotype scoring improved results substantially, but migration of cluster files between sites led to a deterioration of results, suggesting that local adjustment of cluster files is required on a site-per-site basis. Information relating to the markers on the chip is available online at https://ics.hutton.ac.uk/50k.

The KMTNet/K2-C9 (Kepler) Data Release

Science.gov (United States)

Kim, H.-W.; Hwang, K.-H.; Kim, D.-J.; Albrow, M. D.; Cha, S.-M.; Chung, S.-J.; Gould, A.; Han, C.; Jung, Y. K.; Kim, S.-L.; Lee, C.-U.; Lee, D.-J.; Lee, Y.; Park, B.-G.; Pogge, R. W.; Ryu, Y.-H.; Shin, I.-G.; Shvartzvald, Y.; Yee, J. C.; Zang, W.; Zhu, W.; KMTNet Collaboration

2018-05-01

We present Korea Microlensing Telescope Network (KMTNet) light curves for microlensing-event candidates in the Kepler K2 C9 field having peaks within three effective timescales of the Kepler observations. These include 181 “clear microlensing” and 84 “possible microlensing” events found by the KMTNet event finder, plus 56 other events found by OGLE and/or MOA that were not found by KMTNet. All data for the first two classes are immediately available for public use without restriction.

44 CFR 9.6 - Decision-making process.

Science.gov (United States)

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Decision-making process. 9.6... HOMELAND SECURITY GENERAL FLOODPLAIN MANAGEMENT AND PROTECTION OF WETLANDS § 9.6 Decision-making process... protection decision-making process to be followed by the Agency in applying the Orders to its actions. While...

Arsenic activates the expression of 3β-HSD in mouse Leydig cells through repression of histone H3K9 methylation

International Nuclear Information System (INIS)

Alamdar, Ambreen; Xi, Guochen; Huang, Qingyu; Tian, Meiping; Eqani, Syed Ali Musstjab Akber Shah; Shen, Heqing

2017-01-01

Arsenic exposure has been associated with male reproductive dysfunction by disrupting steroidogenesis; however, the roles of epigenetic drivers, especially histone methylation in arsenic-induced steroidogenic toxicity remain not well documented. In this study, we investigated the role of histone H3 lysine 9 (H3K9) methylation in steroidogenesis disturbance in mouse Leydig cells (MLTC-1) due to arsenic exposure. Our results indicated that mRNA and protein expression levels of 3β-hydroxysteroid dehydrogenase (3β-HSD) were both significantly up-regulated while the rest of key genes involved in steroidogenesis were down-regulated. Moreover, arsenic exposure significantly decreased the histone H3K9 di- and tri-methylation (H3K9me2/3) levels in MLTC-1 cells. Since H3K9 demethylation leads to gene activation, we further investigated whether the induction of 3β-HSD expression was ascribed to reduced H3K9 methylation. The results showed that H3K9me2/3 demethylase (JMJD2A) inhibitor, quercetin (Que) significantly attenuated the decrease of H3K9me2/3 and increase of 3β-HSD expression induced by arsenic. To further elucidate the mechanism for the activation of 3β-HSD, we determined the histone H3K9 methylation levels in Hsd3b gene promoter, which also showed significant decrease of H3K9me2/3 in the investigated region after arsenic exposure. Considering these results, we conclude that arsenic exposure induced 3β-HSD up-regulation by suppressing H3K9me2/3 status, which is suggested as a compensatory mechanism for steroidogenic disturbance in MLTC-1 cells. - Highlights: • Epigenetic mechanisms of arsenic-induced male reproductive toxicity remain unclear. • Arsenic disturbs the expression of key steroidogenic genes in MLTC-1 cells. • Histone H3K9 di- and tri-methylation was suppressed in arsenic-exposed cells. • Arsenic activates 3β-HSD expression through repression of histone H3K9 methylation.

Arsenic activates the expression of 3β-HSD in mouse Leydig cells through repression of histone H3K9 methylation

Energy Technology Data Exchange (ETDEWEB)

Alamdar, Ambreen; Xi, Guochen [Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021 (China); Huang, Qingyu, E-mail: qyhuang@iue.ac.cn [Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021 (China); Centre for Epigenetics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M (Denmark); Tian, Meiping [Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021 (China); Eqani, Syed Ali Musstjab Akber Shah [Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021 (China); Public Health and Environment Division, Department of Biosciences, COMSAT Institute of Information & Technology, Islamabad (Pakistan); Shen, Heqing, E-mail: hqshen@iue.ac.cn [Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021 (China)

2017-07-01

Arsenic exposure has been associated with male reproductive dysfunction by disrupting steroidogenesis; however, the roles of epigenetic drivers, especially histone methylation in arsenic-induced steroidogenic toxicity remain not well documented. In this study, we investigated the role of histone H3 lysine 9 (H3K9) methylation in steroidogenesis disturbance in mouse Leydig cells (MLTC-1) due to arsenic exposure. Our results indicated that mRNA and protein expression levels of 3β-hydroxysteroid dehydrogenase (3β-HSD) were both significantly up-regulated while the rest of key genes involved in steroidogenesis were down-regulated. Moreover, arsenic exposure significantly decreased the histone H3K9 di- and tri-methylation (H3K9me2/3) levels in MLTC-1 cells. Since H3K9 demethylation leads to gene activation, we further investigated whether the induction of 3β-HSD expression was ascribed to reduced H3K9 methylation. The results showed that H3K9me2/3 demethylase (JMJD2A) inhibitor, quercetin (Que) significantly attenuated the decrease of H3K9me2/3 and increase of 3β-HSD expression induced by arsenic. To further elucidate the mechanism for the activation of 3β-HSD, we determined the histone H3K9 methylation levels in Hsd3b gene promoter, which also showed significant decrease of H3K9me2/3 in the investigated region after arsenic exposure. Considering these results, we conclude that arsenic exposure induced 3β-HSD up-regulation by suppressing H3K9me2/3 status, which is suggested as a compensatory mechanism for steroidogenic disturbance in MLTC-1 cells. - Highlights: • Epigenetic mechanisms of arsenic-induced male reproductive toxicity remain unclear. • Arsenic disturbs the expression of key steroidogenic genes in MLTC-1 cells. • Histone H3K9 di- and tri-methylation was suppressed in arsenic-exposed cells. • Arsenic activates 3β-HSD expression through repression of histone H3K9 methylation.

Characterization of the antifungal activity of Lactobacillus harbinensis K.V9.3.1Np and Lactobacillus rhamnosus K.C8.3.1I in yogurt.

Science.gov (United States)

Delavenne, Emilie; Cliquet, Sophie; Trunet, Clément; Barbier, Georges; Mounier, Jérôme; Le Blay, Gwenaëlle

2015-02-01

Few antifungal protective cultures adapted to fermented dairy products are commercially available because of the numerous constraints linked to their market implementation. Consumer's demand for naturally preserved food products is growing and the utilization of lactic acid bacteria is a promising way to achieve this goal. In this study, using a 2(5-1) factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contamination amount of a spoilage yeast, on antifungal activities of single and mixed cultures of Lactobacillus rhamnosus K.C8.3.1I and Lactobacillus harbinensis K.V9.3.1Np in yogurt. L. harbinensis K.V9.3.1Np, the most relevant strain with regard to antifungal activity was then studied to determine its minimal inhibitory inoculation rate, its antifungal stability during storage and its impact on yogurt organoleptic properties. We showed that L. harbinensis K.V9.3.1Np maintained a stable antifungal activity over time, which was not affected by initial sucrose, nor by a reduction of the fermentation time. This inhibitory activity was an all-or-nothing phenomenon. Once L. harbinensis K.V9.3.1Np reached a population of ∼ 2.5 × 10(6) cfu/g of yogurt at the time of contamination, total inhibition of the yeast was achieved. We also showed that an inoculation rate of 5 × 10(6) cfu/ml in milk had no detrimental effect on yogurt organoleptic properties. In conclusion, L. harbinensis K.V9.3.1Np is a promising antifungal bioprotective strain for yogurt preservation. Copyright © 2014 Elsevier Ltd. All rights reserved.

Itataia project - Development of the process

International Nuclear Information System (INIS)

Coelho, S.V.

1987-01-01

A process for treating the phosphorus uraniferous ore, from Itataia-CE mine in Brazil, was developed, establishing the basic flow chart for recovery two products: uranium concentrate and phosphoric acid. The developed process consists in physical concentration, chemical separation, solvent extraction, and it presented, in laboratory and pilot scales, recovery levels which assure the project viability technicaly and economicaly. The consolidation of project and the description of installations are presented by a documentary film. (M.C.K.) [pt

Itataia project - Development of the process

International Nuclear Information System (INIS)

Coelho, S.V.

1987-01-01

A process for treating the phosphorous uraniferous ore, from Itataia-CE mine in Brazil, was developed, establishing the basic flow chart for recovery two products: uranium concentrate and phosphoric acid. The developed process consists in physical concentration, chemical separation, solvent extraction, and it presented, in laboratory and pilot scales, recovery leves which assure the project viability technically and economically. The consolidation of project and the description of installations are presented by a documentary film. (M.C.K.) [pt

Technology development life cycle processes.

Energy Technology Data Exchange (ETDEWEB)

Beck, David Franklin

2013-05-01

This report and set of appendices are a collection of memoranda originally drafted in 2009 for the purpose of providing motivation and the necessary background material to support the definition and integration of engineering and management processes related to technology development. At the time there was interest and support to move from Capability Maturity Model Integration (CMMI) Level One (ad hoc processes) to Level Three. As presented herein, the material begins with a survey of open literature perspectives on technology development life cycles, including published data on %E2%80%9Cwhat went wrong.%E2%80%9D The main thrust of the material presents a rational expose%CC%81 of a structured technology development life cycle that uses the scientific method as a framework, with further rigor added from adapting relevant portions of the systems engineering process. The material concludes with a discussion on the use of multiple measures to assess technology maturity, including consideration of the viewpoint of potential users.

Subtask 3.9 - Direct Coal Liquefaction Process Development

Energy Technology Data Exchange (ETDEWEB)

Aulich, Ted; Sharma, Ramesh

2012-07-01

The Energy and Environmental Research Center (EERC), in partnership with the U.S. Department of Energy (DOE) and Accelergy Corporation, an advanced fuels developer with technologies exclusively licensed from ExxonMobil, undertook Subtask 3.9 to design, build, and preliminarily operate a bench-scale direct coal liquefaction (DCL) system capable of converting 45 pounds/hour of pulverized, dried coal to a liquid suitable for upgrading to fuels and/or chemicals. Fabrication and installation of the DCL system and an accompanying distillation system for off-line fractionation of raw coal liquids into 1) a naphtha middle distillate stream for upgrading and 2) a recycle stream was completed in May 2012. Shakedown of the system was initiated in July 2012. In addition to completing fabrication of the DCL system, the project also produced a 500-milliliter sample of jet fuel derived in part from direct liquefaction of Illinois No. 6 coal, and submitted the sample to the Air Force Research Laboratory (AFRL) at Wright Patterson Air Force Base, Dayton, Ohio, for evaluation. The sample was confirmed by AFRL to be in compliance with all U.S. Air Force-prescribed alternative aviation fuel initial screening criteria.

Actinide L-line ED-XRF and Hybrid K-edge Densitometer Spectra Processing

International Nuclear Information System (INIS)

Esbelin, E.

2015-01-01

The analysis laboratory in the CEA Atalante complex at Marcoule (France) performs numerous R and D studies carried out in glove-boxes or in hot cells. Most of the samples are measured in liquid phase, aqueous or organic. The concentration of the main actinides of interest (U, Np, Pu, Am and Cm) are determined by XRF in a hot cell via their L-line X-ray between 13 and 15 keV. In order to limit the counting rate of many radioactive emitters (X-ray and gamma emitters) in the analysis solution and the continuous spectrum, a graphite monochromator is placed between the sample and detector. Commercial or free, the software packages available for processing X-ray spectra are designed and dedicated to a specific instrument and/or do not take into account the specific feature of our system, in other words, the presence of a monochromator. Therefore, a new X-ray analysis software programme was developed for this particular system which takes into account matrix effects corrections. For sample with U and/or Pu in high concentrations, the hybrid K-edge densitometer is used. A new software programme was also developed. For K-edge densitometry spectra processing, no calibration process is used. Spectra processing is based on theoretical equation and uses XCOM database for mass attenuation coefficients. Measured spectra on K-edge densitometer of Rokkasho Safeguards Analytical Laboratory were processed with this software and a very good agreement was found with IDTIMS results. The new graphical user interface allows to manually correct the defined edge. For the XRF spectra processing, new algorithms are used to define the base line and to find/integrate peaks. With these two analytical devices in laboratory, U and Pu concentrations can be measured from 0.5 mg/l to several hundred of g/l. (author)

Search for exotic barton resonances in the final states of K-p and K-d interactions at 2.9 GeV/c

International Nuclear Information System (INIS)

Staab, R.W.

1975-11-01

The reactions K - p → Ψ 0 π - π + K 0 in hydrogen and K - n → Ψ - π - K + in deuterium were analyzed for isotopic spin-3/2 exotic cascade resonance production at a K - beam momentum of 2.9 GeV/c. In addition, the strangeness +1 baryons (Z*'s) were searched for in the reaction K - d → Ψ - Z* at the same beam momentum. The three particle states under consideration lie at the corners of an exotic decuplet. The data came from the Brandeis-Maryland-Syracuse-Tufts collaboration involving two experiments at the 31'' bubble chamber at Brookhaven National Laboratory. Each involved 10 6 pictures with a 30 event/μbarn exposure in hydrogen and a 17 event/μbarn exposure in deuterium

Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.

Science.gov (United States)

Scherer, Paul C; Ding, Yan; Liu, Zhiqing; Xu, Jing; Mao, Haibin; Barrow, James C; Wei, Ning; Zheng, Ning; Snyder, Solomon H; Rao, Feng

2016-03-29

The family of cullin-RING E3 Ligases (CRLs) and the constitutive photomorphogenesis 9 (COP9) signalosome (CSN) form dynamic complexes that mediate ubiquitylation of 20% of the proteome, yet regulation of their assembly/disassembly remains poorly understood. Inositol polyphosphates are highly conserved signaling molecules implicated in diverse cellular processes. We now report that inositol hexakisphosphate (IP6) is a major physiologic determinant of the CRL-CSN interface, which includes a hitherto unidentified electrostatic interaction between the N-terminal acidic tail of CSN subunit 2 (CSN2) and a conserved basic canyon on cullins. IP6, with an EC50 of 20 nM, acts as an intermolecular "glue," increasing cullin-CSN2 binding affinity by 30-fold, thereby promoting assembly of the inactive CRL-CSN complexes. The IP6 synthase, Ins(1,3,4,5,6)P5 2-kinase (IPPK/IP5K) binds to cullins. Depleting IP5K increases the percentage of neddylated, active Cul1 and Cul4A, and decreases levels of the Cul1/4A substrates p27 and p21. Besides dysregulating CRL-mediated cell proliferation and UV-induced apoptosis, IP5K depletion potentiates by 28-fold the cytotoxic effect of the neddylation inhibitor MLN4924. Thus, IP5K and IP6 are evolutionarily conserved components of the CRL-CSN system and are potential targets for cancer therapy in conjunction with MLN4924.

Expansion of plasma of electrically exploding single copper wire under 4.5 kA-9.5 kA/wire

International Nuclear Information System (INIS)

Li Yexun; Yang Libing; Sun Chengwei

2003-01-01

The experimental system for electrically exploding single metal wire has been designed and manufactured. Expansion of the dense plasma column formed from an electrically exploding Cu wire of diameter 30 μm has been studied with a high-speed photographer to obtain the time-dependent radius (R-t) curve. The experimental results demonstrate that the mean expansion rate of the dense plasma column is 1.94 μm/ns, 2.6 μm/ns and 3.75 μm/ns according to the peak pulse current 4.5 kA, 7 kA and 9.5 kA respectively. The results can be beneficial to giving a profound understanding of the early stage of wire-array Z-pinch physics and to improvement on their design

Effects of Synthesis and Processing on the Thermoelectric Properties of Ca3Co4O9+δ

DEFF Research Database (Denmark)

Wu, NingYu; Holgate, Tim; Van Nong, Ngo

In the present study, Ca3Co4O9+δ was synthesized by solid-state and sol-gel reactions followed by spark plasma sintering (SPS) under different conditions such as sintering temperatures, applied pressures and ramping rates. The materials were then characterized with respect to their microstructure......, phase purity and thermoelectric properties. With the identical optimal SPS process, the power factor of about 400 µW/m•K2 and 465 µW/m•K2 (at 800 °C) is measured from samples produced by solid-state and sol-gel reactions respectively, both of these values are higher than the value reported so far....... The thermoelectric performance improvement observed for the solid-state and sol-gel reactions suggests that the particle sizes may be a predominant key parameter of the Ca3Co4O9+δ thermoelectric properties. Smaller particle size (500 nm) as produced in this study by sol-gel synthesis method with optimal SPS process...

Effects of the India–Pakistan border earthquake on the atmospherics at 6 kHz and 9 kHz recorded at Tripura

Directory of Open Access Journals (Sweden)

Sudarsan Barui

2011-04-01

Full Text Available The unusual variations observed in the records of the integrated field intensity of the atmospherics (IFIA at 6 kHz and 9 kHz at Agartala, Tripura, in the north-eastern state of India (latitude, 23˚ N; longitude, 91.4˚ E during the large earthquake on October 8, 2005 at Muzaffarabad (latitude, 34.53˚ N; longitude, 73.58˚ E in Kashmir in Pakistan are here analyzed. Spiky variations in the IFIA at 6 kHz and 9 kHz were observed several days previous to the day of the earthquake (from midnight, September 28, 2005. The effects persisted for some days, decayed gradually, and eventually ceased on October 31, 2005. The spikes are distinctly superimposed on the ambient level

Optimum copper to superconductor ratio in cables for superconducting magnets at 1.9 K

International Nuclear Information System (INIS)

Wolf, R.

1994-01-01

In this paper the optimum copper to superconducting ratio is calculated to prevent quenching for superconducting cables used in accelerator magnets like the LHC dipoles, operating in superfluid helium at 1.9K. The duration of the perturbations leading to a quench are estimated from flux measurements made with pickup coils in the LHC dipole models. The optimum copper to superconducting ratio is then found by studying the minimum quench energy and the influence of the length and the duration or the perturbation and heat transfer to the surroundings. A comparison is made of the behavior at temperatures of 1.9 and 4.3 K

An H3K9/S10 methyl-phospho switch modulates Polycomb and Pol II binding at repressed genes during differentiation.

Science.gov (United States)

Sabbattini, Pierangela; Sjoberg, Marcela; Nikic, Svetlana; Frangini, Alberto; Holmqvist, Per-Henrik; Kunowska, Natalia; Carroll, Tom; Brookes, Emily; Arthur, Simon J; Pombo, Ana; Dillon, Niall

2014-03-01

Methylated histones H3K9 and H3K27 are canonical epigenetic silencing modifications in metazoan organisms, but the relationship between the two modifications has not been well characterized. H3K9me3 coexists with H3K27me3 in pluripotent and differentiated cells. However, we find that the functioning of H3K9me3 is altered by H3S10 phosphorylation in differentiated postmitotic osteoblasts and cycling B cells. Deposition of H3K9me3/S10ph at silent genes is partially mediated by the mitogen- and stress-activated kinases (MSK1/2) and the Aurora B kinase. Acquisition of H3K9me3/S10ph during differentiation correlates with loss of paused S5 phosphorylated RNA polymerase II, which is present on Polycomb-regulated genes in embryonic stem cells. Reduction of the levels of H3K9me3/S10ph by kinase inhibition results in increased binding of RNAPIIS5ph and the H3K27 methyltransferase Ezh1 at silent promoters. Our results provide evidence of a novel developmentally regulated methyl-phospho switch that modulates Polycomb regulation in differentiated cells and stabilizes repressed states.

Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3-HP1-Me(3)K20H4 pathway.

Science.gov (United States)

Bongiorni, Silvia; Pasqualini, Barbara; Taranta, Monia; Singh, Prim B; Prantera, Giorgio

2007-03-15

Using RNA interference (RNAi) we have conducted a functional analysis of the HP1-like chromobox gene pchet2 during embryogenesis of the mealybug Planococcus citri. Knocking down pchet2 expression results in decondensation of the male-specific chromocenter that normally arises from the developmentally-regulated facultative heterochromatinisation of the paternal chromosome complement. Together with the disappearance of the chromocenter the staining levels of two associated histone modifications, tri-methylated lysine 9 of histone H3 [Me(3)K9H3] and tri-methylated lysine 20 of histone H4 [Me(3)K20H4], are reduced to undetectable levels. Embryos treated with double-stranded RNA (dsRNA) targeting pchet2 also exhibit chromosome abnormalities, such as aberrant chromosome condensation, and also the presence of metaphases that contain 'lagging' chromosomes. We conclude that PCHET2 regulates chromosome behavior during metaphase and is a crucial component of a Me(3)K9H3-HP1-Me(3)K20H4 pathway involved in the facultative heterochromatinisation of the (imprinted) paternal chromosome set.

Molecular Targets of Chromatin Repressive Mark H3K9me3 in Primate Progenitor Cells within Adult Neurogenic Niches

Directory of Open Access Journals (Sweden)

Michael R Foret

2014-07-01

Full Text Available Histone 3 Lysine 9 (H3K9 methylation is known to be associated with pericentric heterochromatin and important in genomic stability. In this study, we show that trimethylation at H3K9 (H3K9me3 is enriched in an adult neural stem cell niche- the subventricular zone (SVZ on the walls of the lateral ventricle in both rodent and non-human primate baboon brain. Previous studies have shown that there is significant correlation between baboon and human regarding genomic similarity and brain structure, suggesting that findings in baboon are relevant to human. To understand the function of H3K9me3 in this adult neurogenic niche, we performed genome-wide analyses using ChIP-Seq (chromatin immunoprecipitation and deep-sequencing and RNA-Seq for in vivo SVZ cells purified from baboon brain. Through integrated analyses of ChIP-Seq and RNA-Seq, we found that H3K9me3-enriched genes associated with cellular maintenance, post-transcriptional and translational modifications, signaling pathways, and DNA replication are expressed, while genes involved in axon/neuron, hepatic stellate cell, or immune-response activation are not expressed. As neurogenesis progresses in the adult SVZ, cell fate restriction is essential to direct proper lineage commitment. Our findings highlight that H3K9me3 repression in undifferentiated SVZ cells is engaged in the maintenance of cell type integrity, implicating a role for H3K9me3 as an epigenetic mechanism to control cell fate transition within this adult germinal niche.

Alternative splicing of sept9a and sept9b in zebrafish produces multiple mRNA transcripts expressed throughout development.

Directory of Open Access Journals (Sweden)

Megan L Landsverk

2010-05-01

Full Text Available Septins are involved in a number of cellular processes including cytokinesis and organization of the cytoskeleton. Alterations in human septin-9 (SEPT9 levels have been linked to multiple cancers, whereas mutations in SEPT9 cause the episodic neuropathy, hereditary neuralgic amyotrophy (HNA. Despite its important function in human health, the in vivo role of SEPT9 is unknown.Here we utilize zebrafish to study the role of SEPT9 in early development. We show that zebrafish possess two genes, sept9a and sept9b that, like humans, express multiple transcripts. Knockdown or overexpression of sept9a transcripts results in specific developmental alterations including circulation defects and aberrant epidermal development.Our work demonstrates that sept9 plays an important role in zebrafish development, and establishes zebrafish as a valuable model organism for the study of SEPT9.

Development of 10kW SOFC module

Energy Technology Data Exchange (ETDEWEB)

Hisatome, N.; Nagata, K. [Mitsubishi Heavy Industries, Ltd., Nagasaki (Japan); Kakigami, S. [Electric Power Development Co., Inc., Tokyo (Japan)] [and others

1996-12-31

Mitsubishi Heavy industries, Ltd. (MHI) has been developing tubular type Solid Oxide Fuel Cells (SOFC) since 1984. A 1 kW module of SOFC has been continuously operated for 3,000 hours with 2 scheduled thermal cycles at Electric Power Development Co., Inc. (EPDC) Wakamatsu Power Station in 1993. We have obtained of 34% (HHV as H{sub 2}) module efficiency and deterioration rate of 2% Per 1,000 hours in this field test. As for next step, we have developed 10 kW module in 1995. The 10 kW module has been operated for 5,000 hours continuously. This module does not need heating support to maintain the operation temperature, and the module efficiency was 34% (HHV as H{sub 2}). On the other hand, we have started developing the technology of pressurized SOFC. In 1996, pressurized MW module has been tested at MHI Nagasaki Shipyard & Machinery, Works. We are now planning the development of pressurized 10 kW module.

Genome-wide reduction in H3K9 acetylation during human embryonic stem cell differentiation

Czech Academy of Sciences Publication Activity Database

Krejčí, Jana; Uhlířová, Radka; Galiová-Šustáčková, Gabriela; Kozubek, Stanislav; Šmigová, Jana; Bártová, Eva

2009-01-01

Roč. 219, č. 3 (2009), s. 677-687 ISSN 0021-9541 R&D Projects: GA MŠk(CZ) LC06027; GA MŠk(CZ) LC535; GA ČR(CZ) GA204/06/0978 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702; CEZ:AV0Z50110509 Keywords : hESC * H3K9 acetylation * epigenetics Subject RIV: BO - Biophysics Impact factor: 4.586, year: 2009

Ornithine decarboxylase antizyme induces hypomethylation of genome DNA and histone H3 lysine 9 dimethylation (H3K9me2 in human oral cancer cell line.

Directory of Open Access Journals (Sweden)

Daisuke Yamamoto

2010-09-01

Full Text Available Methylation of CpG islands of genome DNA and lysine residues of histone H3 and H4 tails regulates gene transcription. Inhibition of polyamine synthesis by ornithine decarboxylase antizyme-1 (OAZ in human oral cancer cell line resulted in accumulation of decarboxylated S-adenosylmethionine (dcSAM, which acts as a competitive inhibitor of methylation reactions. We anticipated that accumulation of dcSAM impaired methylation reactions and resulted in hypomethylation of genome DNA and histone tails.Global methylation state of genome DNA and lysine residues of histone H3 and H4 tails were assayed by Methylation by Isoschizomers (MIAMI method and western blotting, respectively, in the presence or absence of OAZ expression. Ectopic expression of OAZ mediated hypomethylation of CpG islands of genome DNA and histone H3 lysine 9 dimethylation (H3K9me2. Protein level of DNA methyltransferase 3B (DNMT3B and histone H3K9me specific methyltransferase G9a were down-regulated in OAZ transfectant.OAZ induced hypomethylation of CpG islands of global genome DNA and H3K9me2 by down-regulating DNMT3B and G9a protein level. Hypomethylation of CpG islands of genome DNA and histone H3K9me2 is a potent mechanism of induction of the genes related to tumor suppression and DNA double strand break repair.

Role of calbindin-D9k in buffering cytosolic free Ca2+ ions in pig duodenal enterocytes.

Science.gov (United States)

Schröder, B; Schlumbohm, C; Kaune, R; Breves, G

1996-05-01

1. The aim of the present study was to test whether the vitamin D-dependent Ca(2+)-binding protein calbindin-D9k could function as an important cytosolic Ca2+ buffer in duodenal enterocytes while facilitating transepithelial active transport of Ca2+ ions. For the investigations we used dual-wavelength, fluorescence ratio imaging, with fura-2 as the Ca(2+)-sensitive dye, to measure changes in cytosolic concentrations of free Ca2+ ions ([Ca2+]i) in isolated pig duodenal enterocytes affected by different cytosolic calbindin-D9k concentrations. 2. Epithelial cells were obtained from weaned piglets with normal calbindin-D9k concentrations (con-piglets), from piglets with low calbindin-D9k levels due to inherited calcitriol deficiency caused by defective renal 25-hydroxycholecalciferol D3-1 alpha-hydroxylase activity (def-piglets), and from piglets with reconstituted calbindin-D9k concentrations, i.e. def-animals treated with high doses of vitamin D3 which elevated plasma calcitriol levels by extrarenal production (def-D3-piglets). Basal levels of [Ca2+]i ranged between 170 and 205 nM and did not differ significantly between the groups. 3. After addition of 5 mM theophylline, the [Ca2+]i in enterocytes from con-piglets doubled during the 10 min incubation. This effect, however, was three times higher in enterocytes from def-piglets compared with those from con-piglets. Similar results were obtained after 4 min incubation of enterocytes from con- and def-piglets in the presence of 1 microM ionomycin. In preparations from def-D3-piglets, ionomycin-induced increases in [Ca2+]i were significantly lower compared with enterocytes from def-piglets and were not different from the control values. 4. From the results, substantial support is given for the hypothesis that one of the major functions of mucosal calbindin-D9k is the effective buffering of Ca2+ ions.

3 kW single stage all-fiber Yb-doped single-mode fiber laser for highly reflective and highly thermal conductive materials processing

Science.gov (United States)

Ikoma, S.; Nguyen, H. K.; Kashiwagi, M.; Uchiyama, K.; Shima, K.; Tanaka, D.

2017-02-01

A 3 kW single stage all-fiber Yb-doped single-mode fiber laser with bi-directional pumping configuration has been demonstrated. Our newly developed high-power LD modules are employed for a high available pump power of 4.9 kW. The length of the delivery fiber is 20 m which is long enough to be used in most of laser processing machines. An output power of 3 kW was achieved at a pump power of 4.23 kW. The slope efficiency was 70%. SRS was able to be suppressed at the same output power by increasing ratio of backward pump power. The SRS level was improved by 5dB when 57% backward pump ratio was adopted compared with the case of 50%. SRS was 35dB below the laser power at the output power of 3 kW even with a 20-m delivery fiber. The M-squared factor was 1.3. Single-mode beam quality was obtained. To evaluate practical utility of the 3 kW single-mode fiber laser, a Bead-on-Plate (BoP) test onto a pure copper plate was executed. The BoP test onto a copper plate was made without stopping or damaging the laser system. That indicates our high power single-mode fiber lasers can be used practically in processing of materials with high reflectivity and high thermal conductivity.

Stress relaxation damage in K9 glass plate irradiated by 1.06μm CW laser

International Nuclear Information System (INIS)

Luo Fu; Sun Chengwei

2001-01-01

Based on the stress relaxation model in 1D planar geometry and the visco-elastic constitutive equation, the temperature and stress histories in the K9 glass samples irradiated by CW laser beams (λ = 1.06 μm) have been calculated. The results indicate that the residual tensile stress due to the stress relaxation effect during cooling after the laser radiation may be greater than the tensile fracture strength of samples, while the maximum compression stress during the laser heating is less than the requirement for compression damage. For a K9 glass window of 3 mm thickness, its damage due to the stress relaxation may be induced by a laser radiation of 0.946 MW/cm 2 for 0.2s . Therefore, the stress relaxation should be regarded as the main mechanism of damage in K9 glass windows while a CW laser beam (λ = 1.06 μm) irradiates it with large spot

PKL/K9, Refill and Reflood Experiment in a Simulated PWR Primary System (PKL)

International Nuclear Information System (INIS)

1981-01-01

reflood process within a pressure vessel considering the influence of the simulated primary loops of a pressurized water reactor (PWR). As to the number of rods and the cross-sections of vessel and components the PKL test-facility is scaled down to 1:134 of a typical West Germany PWR. The elevations and locations have been designed in order to reproduce substantially the original extension. Compared to other cold leg injection tests in the same facility (K5. 3a, K5. 4a) test K9 differs in a uniform radial power profile in the core, an increased temperature of injected Emergency Core Cooling (ECC) water (feed water, from 35 to 53 deg. C), an injection mass flow rate highly reduced to 1:3, and a history of it corresponding to that of a typical US-PWR, on the average somewhat higher maximum initial temperatures in the bundle, and a smaller bundle heating power during the initial phase

Pre-analytical variables of circulating cell-free nucleosomes containing 5-methylcytosine DNA or histone modification H3K9Me3

DEFF Research Database (Denmark)

Rasmussen, Louise; Herzog, Marielle; Rømer, Eva

2016-01-01

Aim: To evaluate pre-analytical variables of circulating cell-free nucleosomes containing 5-methylcytosine DNA (5mC) or histone modification H3K9Me3 (H3K9Me3). Materials and methods: Six studies were designed to assess the possible influence of pre-analytical variables. Study 1: influence of stasis...... significantly lower levels of 5mC or H3K9Me3 compared to levels in healthy individuals. Conclusion: Levels of 5mC or H3K9Me3 appear stable in most pre-analytical settings if blood samples are stored at room temperature until centrifugation....

Your Child's Development: 9 Months

Science.gov (United States)

... Child's Development: 9 Months Print en español El desarrollo de su hijo: 9 meses Nine-month-olds ... item in each hand at the same time Social and Emotional Development might be fearful of strangers ...

9 CFR 381.86 - Inflammatory processes.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Inflammatory processes. 381.86 Section 381.86 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... Carcasses and Parts § 381.86 Inflammatory processes. Any organ or other part of a carcass which is affected...

Effect of Heat Treatment Process on Mechanical Properties and Microstructure of a 9% Ni Steel for Large LNG Storage Tanks

Science.gov (United States)

Zhang, J. M.; Li, H.; Yang, F.; Chi, Q.; Ji, L. K.; Feng, Y. R.

2013-12-01

In this paper, two different heat treatment processes of a 9% Ni steel for large liquefied natural gas storage tanks were performed in an industrial heating furnace. The former was a special heat treatment process consisting of quenching and intercritical quenching and tempering (Q-IQ-T). The latter was a heat treatment process only consisting of quenching and tempering. Mechanical properties were measured by tensile testing and charpy impact testing, and the microstructure was analyzed by optical microscopy, transmission electron microscopy, and x-ray diffraction. The results showed that outstanding mechanical properties were obtained from the Q-IQ-T process in comparison with the Q-T process, and a cryogenic toughness with charpy impact energy value of 201 J was achieved at 77 K. Microstructure analysis revealed that samples of the Q-IQ-T process had about 9.8% of austenite in needle-like martensite, while samples of the Q-T process only had about 0.9% of austenite retained in tempered martensite.

Development program for a 200-kW, cw gyrotron. Quarterly report No. 9, July-September 1981

International Nuclear Information System (INIS)

Tancredi, J.J.; Caplan, M.; Matranga, V.A.; Sandoval, J.J.

1981-01-01

The objective of this program is the design and development of a millimeter-wave device to produce 200 kW of continuous-wave power at 60 GHz. The device, which will be a gyrotron oscillator, will be compatible with power delivery to an electron-cyclotron plasma. Smooth control of rf power output over a 17 dB range is required, and the device should be capable of operation into a severe time-varying rf load mismatch. The superconducting solenoid which had been sent back to the manufacturer because of an open coil, was repaired and returned to Hughes. The gyrotron S/N 1 was successfully operated at a frequency of 59.7 GHz with a power level of 160 kW peak. Diagnostic x-ray measurements of the collector area revealed a reasonable beam power density of 600 W/cm 2 , under worst case conditions of no rf output, and with no auxilary means of spreading the beam. The latter portion of this report period was spent characterizing S/N 1's rf and dc performance and evaluating an anomolous oscillation emitted from the electron gun

Quasi-resonant K-K charge transfer

International Nuclear Information System (INIS)

Hagmann, S.; Cocke, C.L.; Richard, P.; Skutlartz, A.; Kelbch, S.; Schmidt-Boecking, H.; Schuch, R.

1983-01-01

The impact parameter dependence, P(b), of single and double K to K charge transfer have been deduced from the coincidences between K-Auger electrons and scattered particles for F 9+ + Ne and F 9+ + Ne collisions at 10 MeV and 4.4 MeV. The 4.4 MeV single K-K transfer probability exhibits oscillations with b. The P(b) for delta-electron emission is also reported. To obtain more details on the mechanism, K-Auger electron-Ne recoil ion coincidences are measured for both F 8+ and F 9+ projectiles. The relative amounts of recoil ions and of satellite and hypersatellite Auger transitions vary substantially with projectile charge state. 11 references, 11 figures

K{sub 2}NaOsO{sub 5.5} and K{sub 3}NaOs{sub 2}O{sub 9}: The first osmium perovskites containing alkali cations at the 'A' site

Energy Technology Data Exchange (ETDEWEB)

Mogare, Kailash M.; Klein, Wilhelm [Stuttgart, Max-Planck-Institut fuer Festkoerperforschung, Heisenbergstrasse 1, 70569 Stuttgart (Germany); Jansen, Martin, E-mail: M.Jansen@fkf.mpg.de [Stuttgart, Max-Planck-Institut fuer Festkoerperforschung, Heisenbergstrasse 1, 70569 Stuttgart (Germany)

2012-07-15

K{sub 2}NaOsO{sub 5.5} and K{sub 3}NaOs{sub 2}O{sub 9} were obtained from solid-state reactions of potassium superoxide, sodium peroxide and osmium metal at elevated oxygen pressures. K{sub 2}NaOsO{sub 5.5} crystallizes as an oxygen-deficient cubic double perovskite in space group Fm3{sup Macron }m with a=8.4184(5) A and contains isolated OsO{sub 6} octahedra. K{sub 3}NaOs{sub 2}O{sub 9} crystallizes hexagonally in P6{sub 3}/mmc with a=5.9998(4) A and c=14.3053(14) A. K{sub 3}NaOs{sub 2}O{sub 9} consists of face sharing Os{sub 2}O{sub 9} pairs of octahedra. According to magnetic measurements K{sub 2}NaOsO{sub 5.5} is diamagnetic, whereas K{sub 3}NaOs{sub 2}O{sub 9} displays strong antiferromagnetic coupling (T{sub N}=140 K), indicating enhanced magnetic interactions within the octahedral pair. - Graphical abstract: High oxidation states of Os, obtained by high oxygen pressure synthesis, are accommodated in double and triple perovskite matrices. K{sub 3}NaOs{sub 2}O{sub 9} displays enhanced magnetic interactions. Highlights: Black-Right-Pointing-Pointer New osmates containing highly oxidized Os were obtained by high O{sub 2} pressure synthesis. Black-Right-Pointing-Pointer High oxidation states of Os are accommodated in double and triple perovskite matrices. Black-Right-Pointing-Pointer Both compounds represent the first Os perovskites with an alkali metal at the A site. Black-Right-Pointing-Pointer K{sub 3}NaOs{sub 2}O{sub 9} displays enhanced magnetic interactions within the octahedral pair.

The histone H3K9 methylation and RNAi pathways regulate normalnucleolar and repeated DNA organization by inhibiting formation ofextrachromosomal DNAs

Energy Technology Data Exchange (ETDEWEB)

Peng, Jamy C.; Karpen, Gary H.

2006-06-15

In order to identify regulators of nuclear organization, Drosophila mutants in the Su(var)3-9 histone H3K9 methyltransferase, RNAi pathway components, and other regulators of heterochromatin-mediated gene silencing were examined for altered nucleoli and positioning of repeated DNAs. Animals lacking components of the H3K9 methylation and RNAi pathways contained disorganized nucleoli, ribosomal DNA (rDNA) and satellite DNAs. The levels of H3K9 dimethylation (H3K9me2) in chromatin associated with repeated DNAs decreased dramatically in Su(var)3-9 and dcr-2 (dicer-2) mutant tissues compared to wild type. We also observed a substantial increase in extrachromosomal repeated DNAs in mutant tissues. The disorganized nucleolus phenotype depends on the presence of Ligase 4 (Lig4), and ecc DNA formation is not induced by removal of cohesin. We conclude that H3K9 methylation of rDNA and satellites, maintained by Su(var)3-9, HP1, and the RNAi pathway, is necessary for the structural stability of repeated DNAs, which is mediated through suppression of non-homologous end joining (NHEJ). These results suggest a mechanism for how local chromatin structure can regulate genome stability, and the organization of chromosomal elements and nuclear organelles.

20% Efficient Zn0.9Mg0.1O:Al/Zn0.8Mg0.2O/Cu(In,Ga)(S,Se)2 Solar Cell Prepared by All-Dry Process through a Combination of Heat-Light-Soaking and Light-Soaking Processes.

Science.gov (United States)

Chantana, Jakapan; Kato, Takuya; Sugimoto, Hiroki; Minemoto, Takashi

2018-04-04

Development of Cd-free Cu(In,Ga)(S,Se) 2 (CIGSSe)-based thin-film solar cells fabricated by an all-dry process is intriguing to minimize optical loss at a wavelength shorter than 520 nm owing to absorption of the CdS buffer layer and to be easily integrated into an in-line process for cost reduction. Cd-free CIGSSe solar cells are therefore prepared by the all-dry process with a structure of Zn 0.9 Mg 0.1 O:Al/Zn 0.8 Mg 0.2 O/CIGSSe/Mo/glass. It is demonstrated that Zn 0.8 Mg 0.2 O and Zn 0.9 Mg 0.1 O:Al are appropriate as buffer and transparent conductive oxide layers with large optical band gap energy values of 3.75 and 3.80 eV, respectively. The conversion efficiency (η) of the Cd-free CIGSSe solar cell without K-treatment is consequently increased to 18.1%. To further increase the η, the Cd-free CIGSSe solar cell with K-treatment is next fabricated and followed by posttreatment called the heat-light-soaking (HLS) + light-soaking (LS) process, including HLS at 110 °C followed by LS under AM 1.5G illumination. It is disclosed that the HLS + LS process gives rise to not only the enhancement of carrier density but also the decrease in the carrier recombination rate at the buffer/absorber interface. Ultimately, the η of the Cd-free CIGSSe solar cell with K-treatment prepared by the all-dry process is enhanced to the level of 20.0%.

Developing cluster strategy of apples dodol SMEs by integration K-means clustering and analytical hierarchy process method

Science.gov (United States)

Mustaniroh, S. A.; Effendi, U.; Silalahi, R. L. R.; Sari, T.; Ala, M.

2018-03-01

The purposes of this research were to determine the grouping of apples dodol small and medium enterprises (SMEs) in Batu City and to determine an appropriate development strategy for each cluster. The methods used for clustering SMEs was k-means. The Analytical Hierarchy Process (AHP) approach was then applied to determine the development strategy priority for each cluster. The variables used in grouping include production capacity per month, length of operation, investment value, average sales revenue per month, amount of SMEs assets, and the number of workers. Several factors were considered in AHP include industry cluster, government, as well as related and supporting industries. Data was collected using the methods of questionaire and interviews. SMEs respondents were selected among SMEs appels dodol in Batu City using purposive sampling. The result showed that two clusters were formed from five apples dodol SMEs. The 1stcluster of apples dodol SMEs, classified as small enterprises, included SME A, SME C, and SME D. The 2ndcluster of SMEs apples dodol, classified as medium enterprises, consisted of SME B and SME E. The AHP results indicated that the priority development strategy for the 1stcluster of apples dodol SMEs was improving quality and the product standardisation, while for the 2nd cluster was increasing the marketing access.

Development of 1000kW-class MCFC pilot plant

Energy Technology Data Exchange (ETDEWEB)

Ooue, M.; Yasue, H. [MCFC Research Association, Mie (Japan); Takasu, K.; Tsuchitori, T.

1996-12-31

This pilot plant is a part of the New Sunshine Program which has proceeded by the Agency of Industrial Science and Technology of the Ministry of International Trade and Industry. MCFC Research Association is entrusted with the development of the pilot plant, and constructing it at Kawagoe site. Following items will be verified by this pilot plant operation. (a) Development of 250kW class stack and confirmation of stack performance and decay rate. (b) System verification such as basic process, control system and operation characteristics, toward commercialization. (c) To get design data for demonstration plant.

19 CFR 10.9 - Articles exported for processing.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 1 2010-04-01 2010-04-01 false Articles exported for processing. 10.9 Section 10... THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Articles Exported and Returned § 10.9 Articles exported for processing. (a) Except as otherwise provided for in this...

Technology Development And Deployment Of Systems For The Retrieval And Processing Of Remote-Handled Sludge From Hanford K-West Fuel Storage Basin

International Nuclear Information System (INIS)

Raymond, R.E.

2011-01-01

In 2011, significant progress was made in developing and deploying technologies to remove, transport, and interim store remote-handled sludge from the 105-K West Fuel Storage Basin on the Hanford Site in south-central Washington State. The sludge in the 105-K West Basin is an accumulation of degraded spent nuclear fuel and other debris that collected during long-term underwater storage of the spent fuel. In 2010, an innovative, remotely operated retrieval system was used to successfully retrieve over 99.7% of the radioactive sludge from 10 submerged temporary storage containers in the K West Basin. In 2011, a full-scale prototype facility was completed for use in technology development, design qualification testing, and operator training on systems used to retrieve, transport, and store highly radioactive K Basin sludge. In this facility, three separate systems for characterizing, retrieving, pretreating, and processing remote-handled sludge were developed. Two of these systems were successfully deployed in 2011. One of these systems was used to pretreat knockout pot sludge as part of the 105-K West Basin cleanup. Knockout pot sludge contains pieces of degraded uranium fuel ranging in size from 600 μm to 6350 μm mixed with pieces of inert material, such as aluminum wire and graphite, in the same size range. The 2011 pretreatment campaign successfully removed most of the inert material from the sludge stream and significantly reduced the remaining volume of knockout pot product material. Removing the inert material significantly minimized the waste stream and reduced costs by reducing the number of transportation and storage containers. Removing the inert material also improved worker safety by reducing the number of remote-handled shipments. Also in 2011, technology development and final design were completed on the system to remove knockout pot material from the basin and transport the material to an onsite facility for interim storage. This system is scheduled

Development of ferritic-martensitic P9 steel for wrapper application in future SFRs

International Nuclear Information System (INIS)

Choudhary, B.K.; Mathew, M.D.; Isaac Samuel, E.; Moitra, A.

2011-01-01

The paper deals with the outcome of the research and development efforts directed towards the development of ferritic-martensitic P9 steel for wrapper application in future sodium cooled fast reactors with an objective to achieve high fuel burnup and more economical nuclear energy. The important and critical issues involved for the development of P9 wrappers such as optimisation of chemical composition in terms of trace elements like sulphur and phosphorous and appropriate thermo-mechanical treatments along with thermal ageing and irradiation effects on fracture properties have been discussed. Tensile properties evaluated at temperatures ranging from 300 to 873 K on the experimental three heats of P9 steel with different silicon contents and made using primary vacuum induction melting followed by secondary electro slag refining route, have been presented. Fracture behaviour examined mainly in terms of ductile to brittle transition temperature and upper shelf energy provided encouraging results. Based on these investigations, a roadmap has been drawn to make experimental P9 steel wrappers for tests in fast breeder test reactor and prototype fast breeder reactor. (author)

Search for the decay {phi}{yields}K{sup 0}K-bar {sup 0}{gamma} with the KLOE experiment

Energy Technology Data Exchange (ETDEWEB)

Ambrosino, F. [Dipartimento di Scienze Fisiche dell' Universita ' Federico II' , Napoli (Italy); INFN Sezione di Napoli, Napoli (Italy); Antonelli, A.; Antonelli, M. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Archilli, F. [Dipartimento di Fisica dell' Universita ' Tor Vergata' , Roma (Italy); INFN Sezione Roma Tor Vergata, Roma (Italy); Beltrame, P. [Institut fuer Kernphysik, Johannes Gutenberg, Universitaet Mainz (Germany); Bencivenni, G.; Bertolucci, S. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Bini, C. [Dipartimento di Fisica dell' Universita Sapienza di Roma, Roma (Italy); INFN Sezione di Roma, Roma (Italy); Bloise, C. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Bocchetta, S. [Dipartimento di Fisica dell' Universita ' Roma Tre' , Roma (Italy); INFN Sezione Roma Tre, Roma (Italy); Bossi, F. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Branchini, P. [INFN Sezione Roma Tre, Roma (Italy); Capon, G.; Capussela, T. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Ceradini, F. [Dipartimento di Fisica dell' Universita ' Roma Tre' , Roma (Italy); INFN Sezione Roma Tre, Roma (Italy); Ciambrone, P.; De Lucia, E. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); De Santis, A. [Dipartimento di Fisica dell' Universita Sapienza di Roma, Roma (Italy); INFN Sezione di Roma, Roma (Italy); De Simone, P. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); De Zorzi, G. [Dipartimento di Fisica dell' Universita Sapienza di Roma, Roma (Italy); INFN Sezione di Roma, Roma (Italy)] (and others)

2009-08-10

We have searched for the decay {phi}{yields}K{sup 0}K-bar {sup 0}{gamma}, by detecting K{sub S} pairs plus a photon and with the K{sub S}-mesons decaying to {pi}{sup +}{pi}{sup -}, in a sample of about 1.5x10{sup 9}{phi}-decays collected by the KLOE experiment at DA{phi}NE. The reaction proceeds through the intermediate states f{sub 0}(980){gamma}, a{sub 0}(980){gamma}. We find five events with 3.2 events expected from background processes. We obtain the upper limit: BR({phi}{yields}K{sup 0}K-bar {sup 0}{gamma})<1.9x10{sup -8} at 90% CL.

Porcine calbindin-D9k gene: expression in endometrium, myometrium, and placenta in the absence of a functional estrogen response element in intron A.

Science.gov (United States)

Krisinger, J; Jeung, E B; Simmen, R C; Leung, P C

1995-01-01

The expression of Calbindin-D9k (CaBP-9k) in the pig uterus and placenta was measured by Northern blot analysis and reverse transcription polymerase chain reaction (PCR), respectively. Progesterone (P4) administration to ovariectomized pigs decreased CaBP-9k mRNA levels. Expression of endometrial CaBP-9k mRNA was high on pregnancy Days 10-12 and below the detection limit on Days 15 and 18. On Day 60, expression could be detected at low levels. In myometrium and placenta, CaBP-9k mRNA expression was not detectable by Northern analysis using total RNA. Reverse-transcribed RNA from both tissues demonstrated the presence of CaBP-9k transcripts by means of PCR. The partial CaBP-9k gene was amplified by PCR and cloned to determine the sequence of intron A. In contrast to the rat CaBP-9k gene, the pig gene does not contain a functional estrogen response element (ERE) within this region. A similar ERE-like sequence located at the identical location was examined by gel retardation analysis and failed to bind the estradiol receptor. A similar disruption of this ERE-like sequence has been described in the human CaBP-9k gene, which is not expressed at any level in placenta, myometrium, or endometrium. It is concluded that the pig CaBP-9k gene is regulated in these reproductive tissues in a manner distinct from that in rat and human tissues. The regulation is probably due to a regulatory region outside of intron A, which in the rat gene contains the key cis element for uterine expression of the CaBP-9k gene.

Development of the Advanced Energy Design Guide for K-12 Schools -- 50% Energy Savings

Energy Technology Data Exchange (ETDEWEB)

Bonnema, E.; Leach, M.; Pless, S.; Torcellini, P.

2013-02-01

This Technical Support Document (TSD) describes the process and methodology for the development of the Advanced Energy Design Guide for K-12 School Buildings: Achieving 50% Energy Savings Toward a Net Zero Energy Building (AEDG-K12) (ASHRAE et al. 2011a). The AEDG-K12 provides recommendations for achieving 50% whole-building energy savings in K-12 schools over levels achieved by following ANSI/ASHRAE/IESNA Standard 90.1-2004, Energy Standard for Buildings Except Low-Rise Residential Buildings (Standard 90.1-2004) (ASHRAE 2004b). The AEDG-K12 was developed in collaboration with the American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE), the American Institute of Architects (AIA), the Illuminating Engineering Society of North America (IES), the U.S. Green Building Council (USGBC), and the U.S. Department of Energy (DOE).

Exon resequencing of H3K9 methyltransferase complex genes, EHMT1, EHTM2 and WIZ, in Japanese autism subjects

OpenAIRE

Balan, Shabeesh; Iwayama, Yoshimi; Maekawa, Motoko; Toyota, Tomoko; Ohnishi, Tetsuo; Toyoshima, Manabu; Shimamoto, Chie; Esaki, Kayoko; Yamada, Kazuo; Iwata, Yasuhide; Suzuki, Katsuaki; Ide, Masayuki; Ota, Motonori; Fukuchi, Satoshi; Tsujii, Masatsugu

2014-01-01

Background Histone H3 methylation at lysine 9 (H3K9) is a conserved epigenetic signal, mediating heterochromatin formation by trimethylation, and transcriptional silencing by dimethylation. Defective GLP (Ehmt1) and G9a (Ehmt2) histone lysine methyltransferases, involved in mono and dimethylation of H3K9, confer autistic phenotypes and behavioral abnormalities in animal models. Moreover, EHMT1 loss of function results in Kleefstra syndrome, characterized by severe intellectual disability, dev...

Crystallite growth kinetics of TiO2 surface modification with 9 mol% ZnO prepared by a coprecipitation process

International Nuclear Information System (INIS)

Ko, Horng-Huey; Hsi, Chi-Shiung; Wang, Moo-Chin; Zhao, Xiujian

2014-01-01

Highlights: • TiO 2 powder surface modification with 9 mol% ZnO was obtained. • Phase transformation from anatase to rutile was hindered by ZnO added. • Growth kinetic of anatase TiO 2 nanocrystallites in T-9Z powders was described as: D A,9 2 =2.42×10 5 ×exp(-39.9×10 3 /RT). • Growth kinetic of rutile TiO 2 nanocrystallites in T-9Z powders was described as: D R,9 2 =8.49×10 5 ×exp(-47.6×10 3 /RT) rutile TiO 2 . -- Abstract: The nanocrystallite growth of TiO 2 surface modification with 9 mol% ZnO prepared by a coprecipitation process has been studied. Thermogravimetric and differential thermal analysis (TG/DTA), X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and UV–VIS–NIR spectrophotometry have been utilized to characterize the TiO 2 nanocrystallites surface modification with 9 mol% ZnO (denoted by T-9Z). The DTA result shows that the anatase TiO 2 first formed at 533 K and the completion of anatase TiO 2 crystallization occurred at 745 K for the T-9Z freeze-dried precursor powders. XRD results reveal that the anatase and rutile TiO 2 coexist when the T-9Z freeze-dried precursor powders were calcined at 523–973 K for 2 h. When the T-9Z freeze-dried precursor powders were calcined at 973 K for 2 h, rutile TiO 2 was the major phase, and the minor phases were anatase TiO 2 and Zn 2 Ti 3 O 8 . The phase was composed of the rutile TiO 2 and Zn 2 TiO 4 for the T-9Z freeze-dried precursor powders after calcination at 1273 K for 2 h. The growth kinetics of TiO 2 nanocrystallites in T-9Z powders were described as: D A,9 2 =2.42×10 5 ×exp(-39.9×10 3 /RT)and D R,9 2 =8.49×10 5 ×exp(-47.6×10 3 /RT) for anatase and rutile TiO 2 nanocrystallites respectively. The analysis results of UV/VIS/NIR spectra reveal that the T-9Z freeze-dried precursor powders after calcination have a red-shifted effect with increasing calcination temperature and can be used as a UVA-attenuating agent

Ctr9, a Key Component of the Paf1 Complex, Affects Proliferation and Terminal Differentiation in the Developing Drosophila Nervous System

Directory of Open Access Journals (Sweden)

Shahrzad Bahrampour

2016-10-01

Full Text Available The Paf1 protein complex (Paf1C is increasingly recognized as a highly conserved and broadly utilized regulator of a variety of transcriptional processes. These include the promotion of H3K4 and H3K36 trimethylation, H2BK123 ubiquitination, RNA Pol II transcriptional termination, and also RNA-mediated gene silencing. Paf1C contains five canonical protein components, including Paf1 and Ctr9, which are critical for overall complex integrity, as well as Rtf1, Leo1, and Cdc73/Parafibromin(Hrpt2/Hyrax. In spite of a growing appreciation for the importance of Paf1C from yeast and mammalian studies, there has only been limited work in Drosophila. Here, we provide the first detailed phenotypic study of Ctr9 function in Drosophila. We found that Ctr9 mutants die at late embryogenesis or early larval life, but can be partly rescued by nervous system reexpression of Ctr9. We observed a number of phenotypes in Ctr9 mutants, including increased neuroblast numbers, increased nervous system proliferation, as well as downregulation of many neuropeptide genes. Analysis of cell cycle and regulatory gene expression revealed upregulation of the E2f1 cell cycle factor, as well as changes in Antennapedia and Grainy head expression. We also found reduction of H3K4me3 modification in the embryonic nervous system. Genome-wide transcriptome analysis points to additional downstream genes that may underlie these Ctr9 phenotypes, revealing gene expression changes in Notch pathway target genes, cell cycle genes, and neuropeptide genes. In addition, we find significant effects on the gene expression of metabolic genes. These findings reveal that Ctr9 is an essential gene that is necessary at multiple stages of nervous system development, and provides a starting point for future studies of the Paf1C in Drosophila.

Molecular Basis for the Regulation of the H3K4 Methyltransferase Activity of PRDM9

Directory of Open Access Journals (Sweden)

Hong Wu

2013-10-01

Full Text Available PRDM9, a histone lysine methyltransferase, is a key determinant of the localization of meiotic recombination hot spots in humans and mice and the only vertebrate protein known to be involved in hybrid sterility. Here, we report the crystal structure of the PRDM9 methyltransferase domain in complex with a histone H3 peptide dimethylated on lysine 4 (H3K4me2 and S-adenosylhomocysteine (AdoHcy, which provides insights into the methyltransferase activity of PRDM proteins. We show that the genuine substrate of PRDM9 is histone H3 lysine 4 (H3K4 and that the enzyme possesses mono-, di-, and trimethylation activities. We also determined the crystal structure of PRDM9 in its autoinhibited state, which revealed a rearrangement of the substrate and cofactor binding sites by a concerted action of the pre-SET and post-SET domains, providing important insights into the regulatory mechanisms of histone lysine methyltransferase activity.

Parallel k-means++

Energy Technology Data Exchange (ETDEWEB)

2017-04-04

A parallelization of the k-means++ seed selection algorithm on three distinct hardware platforms: GPU, multicore CPU, and multithreaded architecture. K-means++ was developed by David Arthur and Sergei Vassilvitskii in 2007 as an extension of the k-means data clustering technique. These algorithms allow people to cluster multidimensional data, by attempting to minimize the mean distance of data points within a cluster. K-means++ improved upon traditional k-means by using a more intelligent approach to selecting the initial seeds for the clustering process. While k-means++ has become a popular alternative to traditional k-means clustering, little work has been done to parallelize this technique. We have developed original C++ code for parallelizing the algorithm on three unique hardware architectures: GPU using NVidia's CUDA/Thrust framework, multicore CPU using OpenMP, and the Cray XMT multithreaded architecture. By parallelizing the process for these platforms, we are able to perform k-means++ clustering much more quickly than it could be done before.

Histone H3 lysine 9 methyltransferase FvDim5 regulates fungal development, pathogenicity and osmotic stress responses in Fusarium verticillioides.

Science.gov (United States)

Gu, Qin; Ji, Tiantian; Sun, Xiao; Huang, Hai; Zhang, Hao; Lu, Xi; Wu, Liming; Huo, Rong; Wu, Huijun; Gao, Xuewen

2017-10-16

Histone methylation plays important biological roles in eukaryotic cells. Methylation of lysine 9 at histone H3 (H3K9me) is critical for regulating chromatin structure and gene transcription. Dim5 is a lysine histone methyltransferase (KHMTase) enzyme, which is responsible for the methylation of H3K9 in eukaryotes. In the current study, we identified a single ortholog of Neurospora crassa Dim5 in Fusarium verticillioides. In this study, we report that FvDim5 regulates the trimethylation of H3K9 (H3K9me3). The FvDIM5 deletion mutant (ΔFvDim5) showed significant defects in conidiation, perithecium production and fungal virulence. Unexpectedly, we found that deletion of FvDIM5 resulted in increased tolerance to osmotic stresses and upregulated FvHog1 phosphorylation. These results indicate the importance of FvDim5 for the regulation of fungal development, pathogenicity and osmotic stress responses in F. verticillioides. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

In situ monitoring of the hydration process of K-PS geopolymer cement with ESEM

International Nuclear Information System (INIS)

Sun Wei; Zhang Yunsheng; Lin Wei; Liu Zhiyong

2004-01-01

Environmental scanning electron microscope (ESEM) was used to in situ quantitatively study the hydration process of K-PS geopolymer cement under an 80% RH environment. An energy dispersion X-ray analysis (EDXA) was also employed to distinguish the chemical composition of hydration product. The ESEM micrographs showed that metakaolin particles pack loosely at 10 min after mixing, resulting in the existence of many large voids. As hydration proceeds, a lot of gels were seen and gradually precipitated on the surfaces of these particles. At later stage, these particles were wrapped by thick gel layers and their interspaces were almost completely filled. The corresponding EDXA results illustrated that the molar ratios of K/Al increase while Si/Al decrease with the development of hydration. As a result, the molar ratios of K/Al and Si/Al of hydration products at an age of 4 h amounted to 0.99 and 1.49, respectively, which were close to the theoretical values (K/Al=1.0, Si/Al=1.0 for K-PS geopolymer cement paste). In addition, well-developed crystals could not been found at any ages; instead, spongelike amorphous gels were always been observed

Multiple k Nearest Neighbor Query Processing in Spatial Network Databases

DEFF Research Database (Denmark)

Xuegang, Huang; Jensen, Christian Søndergaard; Saltenis, Simonas

2006-01-01

This paper concerns the efficient processing of multiple k nearest neighbor queries in a road-network setting. The assumed setting covers a range of scenarios such as the one where a large population of mobile service users that are constrained to a road network issue nearest-neighbor queries...... for points of interest that are accessible via the road network. Given multiple k nearest neighbor queries, the paper proposes progressive techniques that selectively cache query results in main memory and subsequently reuse these for query processing. The paper initially proposes techniques for the case...... where an upper bound on k is known a priori and then extends the techniques to the case where this is not so. Based on empirical studies with real-world data, the paper offers insight into the circumstances under which the different proposed techniques can be used with advantage for multiple k nearest...

Performance Analysis of Entropy Methods on K Means in Clustering Process

Science.gov (United States)

Dicky Syahputra Lubis, Mhd.; Mawengkang, Herman; Suwilo, Saib

2017-12-01

K Means is a non-hierarchical data clustering method that attempts to partition existing data into one or more clusters / groups. This method partitions the data into clusters / groups so that data that have the same characteristics are grouped into the same cluster and data that have different characteristics are grouped into other groups.The purpose of this data clustering is to minimize the objective function set in the clustering process, which generally attempts to minimize variation within a cluster and maximize the variation between clusters. However, the main disadvantage of this method is that the number k is often not known before. Furthermore, a randomly chosen starting point may cause two points to approach the distance to be determined as two centroids. Therefore, for the determination of the starting point in K Means used entropy method where this method is a method that can be used to determine a weight and take a decision from a set of alternatives. Entropy is able to investigate the harmony in discrimination among a multitude of data sets. Using Entropy criteria with the highest value variations will get the highest weight. Given this entropy method can help K Means work process in determining the starting point which is usually determined at random. Thus the process of clustering on K Means can be more quickly known by helping the entropy method where the iteration process is faster than the K Means Standard process. Where the postoperative patient dataset of the UCI Repository Machine Learning used and using only 12 data as an example of its calculations is obtained by entropy method only with 2 times iteration can get the desired end result.

10 CFR 451.9 - Procedures for processing applications.

Science.gov (United States)

2010-01-01

... 10 Energy 3 2010-01-01 2010-01-01 false Procedures for processing applications. 451.9 Section 451.9 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION RENEWABLE ENERGY PRODUCTION INCENTIVES § 451.9... or operators of qualified renewable energy facilities using solar, wind, ocean, geothermal, and...

NPA4K development system using object-oriented methodology

International Nuclear Information System (INIS)

Jeong, Kwang Seong; Hahn, Do Hee

2000-11-01

NPA4K consists of module programs with several components for various functions. Software components have to be developed systematically by compartment criteria and design method. In this paper, the understandings of a typical Object-Oriented Methodology , UML(Unified Modeling Language), the procedure for NPA4K program development and the architecture for long-term development of NPA4K are introduced

NPA4K development system using object-oriented methodology

Energy Technology Data Exchange (ETDEWEB)

Jeong, Kwang Seong; Hahn, Do Hee

2000-11-01

NPA4K consists of module programs with several components for various functions. Software components have to be developed systematically by compartment criteria and design method. In this paper, the understandings of a typical Object-Oriented Methodology , UML(Unified Modeling Language), the procedure for NPA4K program development and the architecture for long-term development of NPA4K are introduced.

Arsenic activates the expression of 3β-HSD in mouse Leydig cells through repression of histone H3K9 methylation

DEFF Research Database (Denmark)

Alamdar, Ambreen; Xi, Guochen; Huang, Qingyu

2017-01-01

methylation. The results showed that H3K9me2/3 demethylase (JMJD2A) inhibitor, quercetin (Que) significantly attenuated the decrease of H3K9me2/3 and increase of 3β-HSD expression induced by arsenic. To further elucidate the mechanism for the activation of 3β-HSD, we determined the histone H3K9 methylation......Arsenic exposure has been associated with male reproductive dysfunction by disrupting steroidogenesis; however, the roles of epigenetic drivers, especially histone methylation in arsenic-induced steroidogenic toxicity remain not well documented. In this study, we investigated the role of histone H3...... lysine 9 (H3K9) methylation in steroidogenesis disturbance in mouse Leydig cells (MLTC-1) due to arsenic exposure. Our results indicated that mRNA and protein expression levels of 3β-hydroxysteroid dehydrogenase (3β-HSD) were both significantly up-regulated while the rest of key genes involved...

21 CFR 886.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 886.9 Section 886.9 Food and Drugs FOOD AND DRUG... Provisions § 886.9 Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act...

21 CFR 872.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 872.9 Section 872.9 Food and Drugs FOOD AND DRUG... Provisions § 872.9 Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act...

21 CFR 888.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 888.9 Section 888.9 Food and Drugs FOOD AND DRUG... Provisions § 888.9 Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act...

21 CFR 892.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

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2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 892.9 Section 892.9 Food and Drugs FOOD AND DRUG... Provisions § 892.9 Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act...

21 CFR 882.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 882.9 Section 882.9 Food and Drugs FOOD AND DRUG... Provisions § 882.9 Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act...

K-factors in the deep inelastic Compton and Drell-Yan processes

International Nuclear Information System (INIS)

Czechowski, A.; Krawczyk, M.

1983-01-01

The associated production of hadrons is used to answer the question about the nature of large QCD corrections for hard processes. The results of the order α s calculation of K-factors for the deep inelastic Compton process and some predictions for the large p perpendicular to Drell-Yan pair production in hadron-hadron collision are presented. In the soft gluon approximation a significant difference between K-factors corresponding to different inclusive distributions in each of these processes is found. (author)

Process Evaluation for Improving K12 Program Effectiveness: Case Study of a National Institutes of Health Building Interdisciplinary Research Careers in Women's Health Research Career Development Program.

Science.gov (United States)

Raymond, Nancy C; Wyman, Jean F; Dighe, Satlaj; Harwood, Eileen M; Hang, Mikow

2018-06-01

Process evaluation is an important tool in quality improvement efforts. This article illustrates how a systematic and continuous evaluation process can be used to improve the quality of faculty career development programs by using the University of Minnesota's Building Interdisciplinary Research Careers in Women's Health (BIRCWH) K12 program as an exemplar. Data from a rigorous process evaluation incorporating quantitative and qualitative measurements were analyzed and reviewed by the BIRCWH program leadership on a regular basis. Examples are provided of how this evaluation model and processes were used to improve many aspects of the program, thereby improving scholar, mentor, and advisory committee members' satisfaction and scholar outcomes. A rigorous evaluation plan can increase the effectiveness and impact of a research career development plan.

Inhibition of H3K9 methyltransferase G9a induces autophagy and apoptosis in oral squamous cell carcinoma

Energy Technology Data Exchange (ETDEWEB)

Ren, Aishu; Qiu, Yu [Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, 401147 (China); Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, 401147 (China); Cui, Hongjuan [State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716 (China); Fu, Gang, E-mail: fg.ras@hotmail.com [Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, 401147 (China); Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, 401147 (China)

2015-03-27

Objective: To explore whether inhibition of H3K9 Methyltransferase G9a could exert an antitumoral effect in oral squamous cell carcinoma (OSCC). Materials and methods: First we checked G9a expression in two OSCC cell lines Tca8113 and KB. Next we used a special G9a inhibitor BIX01294 (BIX) to explore the effect of inhibition of G9a on OSCC in vitro. Cell growth was tested by typlan blue staining, MTT assay and Brdu immunofluorescence staining. Cell autophagy was examined by monodansylcadaverine (MDC) staining, LC3-II immunofluorescence staining and LC3-II western blot assay. Cell apoptosis was checked by FITC Annexin-V and PI labeling, tunnel staining and caspase 3 western blot assay. Finally, the effect of inhibition of G9a on clonogenesis and tumorigenesis capacity of OSCC was analyzed by soft agar growth and xenograft model. Results: Here we showed that G9a was expressed in both Tca8113 and KB cells. Inhibition of G9a using BIX significantly reduced cell growth and proliferation in Tca8113 and KB. Inhibition of G9a induced cell autophagy with conversion of LC3-I to LC3-II and cell apoptosis with the expression of cleaved caspase 3. We also found that inhibition of G9a reduced colony formation in soft agar and repressed tumor growth in mouse xenograph model. Conclusion: Our results suggested that G9a might be a potential epigenetic target for OSCC treatment. - Highlights: • Inhibition of G9a reduced cell growth and proliferation in OSCC cells. • Inhibition of G9a induces autophagy and apoptosis in OSCC cells. • Inhibition of G9a repressed tumor growth in mouse xenograph model.

The goya mouse mutant reveals distinct newly identified roles for MAP3K1 in the development and survival of cochlear sensory hair cells.

Science.gov (United States)

Parker, Andrew; Cross, Sally H; Jackson, Ian J; Hardisty-Hughes, Rachel; Morse, Susan; Nicholson, George; Coghill, Emma; Bowl, Michael R; Brown, Steve D M

2015-12-01

Mitogen-activated protein kinase, MAP3K1, plays an important role in a number of cellular processes, including epithelial migration during eye organogenesis. In addition, studies in keratinocytes indicate that MAP3K1 signalling through JNK is important for actin stress fibre formation and cell migration. However, MAP3K1 can also act independently of JNK in the regulation of cell proliferation and apoptosis. We have identified a mouse mutant, goya, which exhibits the eyes-open-at-birth and microphthalmia phenotypes. In addition, these mice also have hearing loss. The goya mice carry a splice site mutation in the Map3k1 gene. We show that goya and kinase-deficient Map3k1 homozygotes initially develop supernumerary cochlear outer hair cells (OHCs) that subsequently degenerate, and a progressive profound hearing loss is observed by 9 weeks of age. Heterozygote mice also develop supernumerary OHCs, but no cellular degeneration or hearing loss is observed. MAP3K1 is expressed in a number of inner-ear cell types, including outer and inner hair cells, stria vascularis and spiral ganglion. Investigation of targets downstream of MAP3K1 identified an increase in p38 phosphorylation (Thr180/Tyr182) in multiple cochlear tissues. We also show that the extra OHCs do not arise from aberrant control of proliferation via p27KIP1. The identification of the goya mutant reveals a signalling molecule involved with hair-cell development and survival. Mammalian hair cells do not have the ability to regenerate after damage, which can lead to irreversible sensorineural hearing loss. Given the observed goya phenotype, and the many diverse cellular processes that MAP3K1 is known to act upon, further investigation of this model might help to elaborate upon the mechanisms underlying sensory hair cell specification, and pathways important for their survival. In addition, MAP3K1 is revealed as a new candidate gene for human sensorineural hearing loss. © 2015. Published by The Company of

The goya mouse mutant reveals distinct newly identified roles for MAP3K1 in the development and survival of cochlear sensory hair cells

Directory of Open Access Journals (Sweden)

Andrew Parker

2015-12-01

Full Text Available Mitogen-activated protein kinase, MAP3K1, plays an important role in a number of cellular processes, including epithelial migration during eye organogenesis. In addition, studies in keratinocytes indicate that MAP3K1 signalling through JNK is important for actin stress fibre formation and cell migration. However, MAP3K1 can also act independently of JNK in the regulation of cell proliferation and apoptosis. We have identified a mouse mutant, goya, which exhibits the eyes-open-at-birth and microphthalmia phenotypes. In addition, these mice also have hearing loss. The goya mice carry a splice site mutation in the Map3k1 gene. We show that goya and kinase-deficient Map3k1 homozygotes initially develop supernumerary cochlear outer hair cells (OHCs that subsequently degenerate, and a progressive profound hearing loss is observed by 9 weeks of age. Heterozygote mice also develop supernumerary OHCs, but no cellular degeneration or hearing loss is observed. MAP3K1 is expressed in a number of inner-ear cell types, including outer and inner hair cells, stria vascularis and spiral ganglion. Investigation of targets downstream of MAP3K1 identified an increase in p38 phosphorylation (Thr180/Tyr182 in multiple cochlear tissues. We also show that the extra OHCs do not arise from aberrant control of proliferation via p27KIP1. The identification of the goya mutant reveals a signalling molecule involved with hair-cell development and survival. Mammalian hair cells do not have the ability to regenerate after damage, which can lead to irreversible sensorineural hearing loss. Given the observed goya phenotype, and the many diverse cellular processes that MAP3K1 is known to act upon, further investigation of this model might help to elaborate upon the mechanisms underlying sensory hair cell specification, and pathways important for their survival. In addition, MAP3K1 is revealed as a new candidate gene for human sensorineural hearing loss.

In silico probing and biological evaluation of SETDB1/ESET-targeted novel compounds that reduce tri-methylated histone H3K9 (H3K9me3) level

Science.gov (United States)

Park, Insun; Hwang, Yu Jin; Kim, TaeHun; Viswanath, Ambily Nath Indu; Londhe, Ashwini M.; Jung, Seo Yun; Sim, Kyoung Mi; Min, Sun-Joon; Lee, Ji Eun; Seong, Jihye; Kim, Yun Kyung; No, Kyoung Tai; Ryu, Hoon; Pae, Ae Nim

2017-10-01

ERG-associated protein with the SET domain (ESET/SET domain bifurcated 1/SETDB1/KMT1E) is a histone lysine methyltransferase (HKMT) and it preferentially tri-methylates lysine 9 of histone H3 (H3K9me3). SETDB1/ESET leads to heterochromatin condensation and epigenetic gene silencing. These functional changes are reported to correlate with Huntington's disease (HD) progression and mood-related disorders which make SETDB1/ESET a viable drug target. In this context, the present investigation was performed to identify novel peptide-competitive small molecule inhibitors of the SETDB1/ESET by a combined in silico-in vitro approach. A ligand-based pharmacophore model was built and employed for the virtual screening of ChemDiv and Asinex database. Also, a human SETDB1/ESET homology model was constructed to supplement the data further. Biological evaluation of the selected 21 candidates singled out 5 compounds exhibiting a notable reduction of the H3K9me3 level via inhibitory potential of SETDB1/ESET activity in SETDB1/ESET-inducible cell line and HD striatal cells. Later on, we identified two compounds as final hits that appear to have neuronal effects without cytotoxicity based on the result from MTT assay. These compounds hold the calibre to become the future lead compounds and can provide structural insights into more SETDB1/ESET-focused drug discovery research. Moreover, these SETDB1/ESET inhibitors may be applicable for the preclinical study to ameliorate neurodegenerative disorders via epigenetic regulation.

In silico probing and biological evaluation of SETDB1/ESET-targeted novel compounds that reduce tri-methylated histone H3K9 (H3K9me3) level.

Science.gov (United States)

Park, Insun; Hwang, Yu Jin; Kim, TaeHun; Viswanath, Ambily Nath Indu; Londhe, Ashwini M; Jung, Seo Yun; Sim, Kyoung Mi; Min, Sun-Joon; Lee, Ji Eun; Seong, Jihye; Kim, Yun Kyung; No, Kyoung Tai; Ryu, Hoon; Pae, Ae Nim

2017-10-01

ERG-associated protein with the SET domain (ESET/SET domain bifurcated 1/SETDB1/KMT1E) is a histone lysine methyltransferase (HKMT) and it preferentially tri-methylates lysine 9 of histone H3 (H3K9me3). SETDB1/ESET leads to heterochromatin condensation and epigenetic gene silencing. These functional changes are reported to correlate with Huntington's disease (HD) progression and mood-related disorders which make SETDB1/ESET a viable drug target. In this context, the present investigation was performed to identify novel peptide-competitive small molecule inhibitors of the SETDB1/ESET by a combined in silico-in vitro approach. A ligand-based pharmacophore model was built and employed for the virtual screening of ChemDiv and Asinex database. Also, a human SETDB1/ESET homology model was constructed to supplement the data further. Biological evaluation of the selected 21 candidates singled out 5 compounds exhibiting a notable reduction of the H3K9me3 level via inhibitory potential of SETDB1/ESET activity in SETDB1/ESET-inducible cell line and HD striatal cells. Later on, we identified two compounds as final hits that appear to have neuronal effects without cytotoxicity based on the result from MTT assay. These compounds hold the calibre to become the future lead compounds and can provide structural insights into more SETDB1/ESET-focused drug discovery research. Moreover, these SETDB1/ESET inhibitors may be applicable for the preclinical study to ameliorate neurodegenerative disorders via epigenetic regulation.

Development of a perturbation code, PERT-K, for hexagonal core geometry

Energy Technology Data Exchange (ETDEWEB)

Kim, Taek Kyum; Kim, Sang Ji; Song, Hoon; Kim, Young Il; Kim, Young Jin [Korea Atomic Energy Research Institute, Taejon (Korea)

1999-01-01

A perturbation code for hexagonal core geometry has been developed based on Nodal Expansion Method. By using relevant output files of DIF3D code, it can calculate the reactivity changes caused by perturbation in composition or/and neutron cross section libraries. The accuracy of PERT-K code has been validated by calculating the reactivity changes due to fuel composition change, the sodium void coefficients, and the sample reactivity worths of BFS-73-1 critical experiments. In the case of 10% reduction in all fuel isotopics at a assembly located in the outer core, PERT-K computation agrees with the direct computation by DIF3D within 60 pcm. The sample reactivity worths of BFS-73-1 critical experiments are predicted with PERT-K code within the experimental error bounds. For 100% sodium void occurrence at the inner core, the maximum difference of reactivity changes between PERT-K and direct DIF3D computations is less than 40 pcm. On the other hand, the same sodium void condition at the outer core leads to a difference of reactivity change greater than 400 pcm. However, as sodium voiding becomes near zero value, the difference becomes less and rapidly falls within the acceptable bound, i.e. 40 pcm. (author). 11 refs., 9 figs., 6 tabs.

Effect of tumbling time, injection rate and k-carrageenan addition on processing, textural and color characteristics of pork Biceps femoris muscle

Directory of Open Access Journals (Sweden)

Livia PATRAŞCU

2013-08-01

Full Text Available The effect of tumbling time (0-9 hours, injection rate (20-50% and k carrageenan addition (0.25 - 0.5% on quality characteristics of cooked pork Biceps femoris muscle have been studied. Properties of injected and tumbled meat samples were determined by measuring processing characteristics (tumbling yield, cooking yield and expressible moisture, color (L*, a*, b*, Hue angle and Chroma and texture (firmness, toughness, adhesiveness, work of adhesion and fracturability. Increasing tumbling time up to 9 h led to better hydration properties and increased the cooking yield for all samples, both with 0.25% and 0.5% of k-carrageenan addition. It also decreased the firmness and toughness of the evaluated samples. Biceps femoris samples containing a higher level of k-carrageenan were tenderer than those containing less polysaccharide. Neither injection rate nor tumbling time affected the color components of the analyzed samples.

Acetylated Histone H3K9 is associated with meiotic recombination hotspots, and plays a role in recombination redundantly with other factors including the H3K4 methylase Set1 in fission yeast

Science.gov (United States)

Yamada, Shintaro; Ohta, Kunihiro; Yamada, Takatomi

2013-01-01

Histone modifications are associated with meiotic recombination hotspots, discrete sites with augmented recombination frequency. For example, trimethylation of histone H3 lysine4 (H3K4me3) marks most hotspots in budding yeast and mouse. Modified histones are known to regulate meiotic recombination partly by promoting DNA double-strand break (DSB) formation at hotspots, but the role and precise landscape of involved modifications remain unclear. Here, we studied hotspot-associated modifications in fission yeast and found general features: acetylation of H3 lysine9 (H3K9ac) is elevated, and H3K4me3 is not significantly enriched. Mutating H3K9 to non-acetylatable alanine mildly reduced levels of the DSB-inducing protein Rec12 (the fission yeast homologue of Spo11) and DSB at hotspots, indicating that H3K9ac may be involved in DSB formation by enhancing the interaction between Rec12 and hotspots. In addition, we found that the lack of the H3K4 methyltransferase Set1 generally increased Rec12 binding to chromatin but partially reduced DSB formation at some loci, suggesting that Set1 is also involved in DSB formation. These results suggest that meiotic DSB formation is redundantly regulated by multiple chromatin-related factors including H3K9ac and Set1 in fission yeast. PMID:23382177

Final report on 9 kW Stirling Engine for biogas and natural gas

DEFF Research Database (Denmark)

Carlsen, Henrik; Bovin, Jonas Kabell

2001-01-01

The need for a simple and robust engine for natural gas and low quality gas has resulted in the design of a single cylinder, hermetic Stirling engine, which has an electric power output of 9 kW. Two engines have been built. One engine is intended for natural gas as fuel and the other is intended...... eliminates guiding forces on the pistons and the need for X-heads. Grease lubricated needle and ball bearings are used in the kinematic crank mechanism in order to avoid oil penetrating into the cylinder volumes. Working gas is Helium at 8 MPa mean pressure. The engine produce up to 11 kW of shaft power...... corresponding to approximately 10 kW of electric power. The design target was an efficiency of 26 % based on lower heat content of the gas to electricity, but only 24% were obtained. The decrease of efficiency is caused by inhomogeneous capacity flows in the air preheater and insufficient insulation...

Development of new measurement and analytical method to trace 41K for long-term

International Nuclear Information System (INIS)

Yuita, Koichi; Miyagawa, Saburo

1999-01-01

To establish a control technique of fertilizer application for resource-saving and environmental conservation, non-radiative 41 K tracer method in the field has been developed. In this paper, potassium silicate fertilizer, one of slow-release fertilizer, was synthesized and marked by 41 K. Potassium silicate fertilizer with natural abundance of 41 K/ 39 K was prepared by mixing 76.2 g fly ash, 23.4 g potassium carbonate and 7.2 g magnesium hydroxide and colicinating the mixture for 47 min at 850degC. The product was satisfied the conditions of potasium silicate fertilizer. Then, the potassium silicate fertilizer marked with 41 K was synthesized by calicinating the mixture 1.85 KCl (6.77% 41 K and 93.23% 39 K) and 11.45 g 41 KCl under the above conditions. 41 K excess% of product fertilizer was about -2.00%, which is possible to trace 41 K of crop plant of soil culture pot test during the growing process. (S.Y.)

The PR-Set7 binding domain of Riz1 is required for the H4K20me1-H3K9me1 trans-tail ‘histone code’ and Riz1 tumor suppressor function

Science.gov (United States)

Congdon, Lauren M.; Sims, Jennifer K.; Tuzon, Creighton T.; Rice, Judd C.

2014-01-01

PR-Set7/Set8/KMT5a is the sole histone H4 lysine 20 monomethyltransferase (H4K20me1) in metazoans and is essential for proper cell division and genomic stability. We unexpectedly discovered that normal cellular levels of monomethylated histone H3 lysine 9 (H3K9me1) were also dependent on PR-Set7, but independent of its catalytic activity. This observation suggested that PR-Set7 interacts with an H3K9 monomethyltransferase to establish the previously reported H4K20me1-H3K9me1 trans-tail ‘histone code’. Here we show that PR-Set7 specifically and directly binds the C-terminus of the Riz1/PRDM2/KMT8 tumor suppressor and demonstrate that the N-terminal PR/SET domain of Riz1 preferentially monomethylates H3K9. The PR-Set7 binding domain was required for Riz1 nuclear localization and maintenance of the H4K20me1-H3K9me1 trans-tail ‘histone code’. Although Riz1 can function as a repressor, Riz1/H3K9me1 was dispensable for the repression of genes regulated by PR-Set7/H4K20me1. Frameshift mutations resulting in a truncated Riz1 incapable of binding PR-Set7 occur frequently in various aggressive cancers. In these cancer cells, expression of wild-type Riz1 restored tumor suppression by decreasing proliferation and increasing apoptosis. These phenotypes were not observed in cells expressing either the Riz1 PR/SET domain or PR-Set7 binding domain indicating that Riz1 methyltransferase activity and PR-Set7 binding domain are both essential for Riz1 tumor suppressor function. PMID:24423864

An oil-in-water self-assembly synthesis, characterization and photocatalytic properties of nano Ag@AgCl surface-sensitized K2Ti4O9

International Nuclear Information System (INIS)

Liang, Yinghua; Lin, Shuanglong; Liu, Li; Hu, Jinshan; Cui, Wenquan

2014-01-01

Highlights: • The plasmatic Ag@AgCl surface-sensitized K 2 Ti 4 O 9 composite photocatalysts. • Ag@AgCl greatly increased visible light absorption for K 2 Ti 4 O 9 . • The photocatalysts exhibited enhanced photocatalytic dye degradation. - Abstract: Nano-sized plasmonic Ag@AgCl surface-sensitized K 2 Ti 4 O 9 composite photocatalysts (hereafter designated as Ag@AgCl/K 2 Ti 4 O 9 ) was synthesized via a facile oil-in-water self-assembly method. The photocatalytic activity of the prepared materials for RhB (Rhodamine B) degradation was examined under visible light irradiation. The results reveal that the size of Ag@AgCl, which evenly dispersed on the surface of K 2 Ti 4 O 9 , distributes about 20–50 nm. The UV–vis diffuse reflectance spectra indicate that Ag@AgCl/K 2 Ti 4 O 9 samples have a significantly enhanced optical absorption in 380–700 nm. The photocatalytic activities of the Ag@AgCl/K 2 Ti 4 O 9 samples increase first and then decrease with increasing amount of loading Ag@AgCl and the Ag@AgCl(20 wt.%)/K 2 Ti 4 O 9 sample exhibits the best photocatalytic activity and 94.47% RhB was degraded after irradiation for 2 h. Additionally, studies performed using radical scavengers indicated that O 2 · − and Cl 0 acted as the main reactive species. The electronic interaction was systematically studied and confirmed by the photo-electrochemical measurements

Prompt $K_{S}^{0}$ production in $pp$ collisions at $\\sqrt{s}$ = 0.9 TeV

CERN Document Server

Aaij, R; Adeva, B; Adinolfi, M; Adrover, C; Affolder, A; Agari, M; Ajaltouni, Z; Albrecht, J; Alessio, F; Alexander, M; Alfonsi, M; Alvarez Cartelle, P; Alves Jr, A.A; Amato, S; Amhis, Y; Amoraal, J; Anderson, J; Antunes Nobrega, R; Appleby, R; Aquines Gutierrez, O; Arefyev, A; Arrabito, L; Artuso, M; Aslanides, E; Auriemma, G; Bachmann, S; Bagaturia, Y; Bailey, D.S; Balagura, V; Baldini, W; Barber, G; Barham, C; Barlow, R.J; Barsuk, S; Basiladze, S; Bates, A; Bauer, C; Bauer, Th; Bay, A; Bediaga, I; Bellunato, T; Belous, K; Belyaev, I; Benayoun, M; Bencivenni, G; Bernet, R; Bernhard, R.P; Bettler, M-O; van Beuzekom, M; Bibby, J.H; Bifani, S; Bizzeti, A; Bjrnstad, P.M; Blake, T; Blanc, F; Blanks, C; Blouw, J; Blusk, S; Bobrov, A; Bocci, V; Bochin, B; Bonaccorsi, E; Bondar, A; Bondar, N; Bonivento, W; Borghi, S; Borgia, A; Bos, E; Bowcock, T.J.V; Bozzi, C; Brambach, T; van den Brand, J; Brarda, L; Bressieux, J; Brisbane, S; Britsch, M; Brook, N.H; Brown, H; Brusa, S; Buchler-Germann, A; Bursche, A; Buytaert, J; Cadeddu, S; Caicedo Carvajal, J.M; Callot, O; Calvi, M; Calvo Gomez, M; Camboni, A; Cameron, W; Camilleri, L; Campana, P; Carbone, A; Carboni, G; Cardinale, R; Cardini, A; Carroll, J; Carson, L; Carvalho Akiba, K; Casse, G; Cattaneo, M; Chadaj, B; Charles, M; Charpentier, Ph; Cheng, J; Chiapolini, N; Chlopik, A; Christiansen, J; Ciambrone, P; Cid Vidal, X; Clark, P.J; Clarke, P.E.L; Clemencic, M; Cliff, H.V; Closier, J; Coca, C; Coco, V; Cogan, J; Collins, P; Comerma-Montells, A; Constantin, F; Conti, G; Contu, A; Cooke, P; Coombes, M; Corajod, B; Corti, G; Cowan, G.A; Currie, R; DAlmagne, B; DAmbrosio, C; DAntone, I; Da Silva, W; Dane, E; David, P; De Bonis, I; De Capua, S; De Cian, M; De Lorenzi, F; De Miranda, J.M; De Paula, L; De Simone, P; Decamp, D; Decreuse, G; Degaudenzi, H; Deissenroth, M; Del Buono, L; Densham, C.J; Deplano, C; Deschamps, O; Dettori, F; Dickens, J; Dijkstra, H; Dima, M; Donleavy, S; Dornan, P; Dossett, D; Dovbnya, A; Dumps, R; Dupertuis, F; Dwyer, L; Dzhelyadin, R; Eames, C; Easo, S; Egede, U; Egorychev, V; Eidelman, S; van Eijk, D; Eisele, F; Eisenhardt, S; Eklund, L; d'Enterria, D; Esperante Pereira, D; Est`eve, L; Fanchini, E; Farber, C; Fardell, G; Farinelli, C; Farry, S; Fave, V; Felici, G; Fernandez Albor, V; Ferro-Luzzi, M; Filippov, S; Fitzpatrick, C; Flegel, W; Fontanelli, F; Forti, C; Forty, R; Fournier, C; Franek, B; Frank, M; Frei, C; Frosini, M; Fungueirino Pazos, J.L; Furcas, S; Gallas Torreira, A; Galli, D; Gandelman, M; Gandini, P; Gao, Y; Garnier, J-C; Garrido, L; Gascon, D; Gaspar, C; Gaspar De Valenzuela Cue, A; Gassner, J; Gauvin, N; Gavillet, P; Gersabeck, M; Gershon, T; Ghez, Ph; Gibson, V; Gilitsky, Yu; Gligorov, V.V; Gobel, C; Golubkov, D; Golutvin, A; Gomes, A; Gong, G; Gong, H; Gordon, H; Grabalosa Gandara, M; Gracco, V; Graciani Diaz, R; Granado Cardoso, L.A; Grauges, E; Graziani, G; Grecu, A; Gregson, S; Guerrer, G; Gui, B; Gushchin, E; Guz, Yu; Guzik, Z; Gys, T; Haefeli, G; Haines, S.C; Hampson, T; Hansmann-Menzemer, S; Harji, R; Harnew, N; Harrison, P.F; He, J; Hennessy, K; Henrard, P; Hernando Morata, J.A; van Herwijnen, E; Hicheur, A; Hicks, E; Hilke, H.J; Hofmann, W; Holubyev, K; Hopchev, P; Hulsbergen, W; Hunt, P; Huse, T; Huston, R.S; Hutchcroft, D; Iacoangeli, F; Iakovenko, V; Iglesias Escudero, C; Ilgner, C; Imong, J; Jacobsson, R; Jahjah Hussein, M; Jamet, O; Jans, E; Jansen, F; Jaton, P; Jean-Marie, B; John, M; Johnson, D; Jones, C.R; Jost, B; Kapusta, F; Karbach, T.M; Kashchuk, A; Katvars, S; Keaveney, J; Kerzel, U; Ketel, T; Keune, A; Khalil, S; Khanji, B; Kim, Y.M; Knecht, M; Koblitz, S; Konoplyannikov, A; Koppenburg, P; Korolev, M; Kozlinskiy, A; Kravchuk, L; Kristic, R; Krocker, G; Krokovny, P; Kruse, F; Kruzelecki, K; Kucharczyk, M; Kudryashov, I; Kukulak, S; Kumar, R; Kvaratskheliya, T; La Thi, V.N; Lacarrere, D; Lai, A; Lambert, R.W; Lanfranchi, G; Langenbruch, C; Latham, T; Le Gac, R; Lees, J-P; Lef`evre, R; Leflat, A; Lefrancois, J; Lehner, F; Lenzi, M; Leroy, O; Lesiak, T; Li, L; Li, Y.Y; Li Gioi, L; Libby, J; Lieng, M; Lindner, R; Lindsey, S; Linn, C; Liu, B; Liu, G; Lochner, S; Lopes, J.H; Lopez Asamar, E; Lopez-March, N; Loveridge, P; Luisier, J; Mcharek, B; Machefert, F; Machikhiliyan, I.V; Maciuc, F; Maev, O; Magnin, J; Maier, A; Malde, S; Mamunur, R.M.D; Manca, G; Mancinelli, G; Mangiafave, N; Marconi, U; Marki, R; Marks, J; Martellotti, G; Martens, A; Martin, L; Martinez Santos, D; Massaferri, A; Mathe, Z; Matteuzzi, C; Matveev, V; Maurice, E; Maynard, B; Mazurov, A; McGregor, G; McNulty, R; Mclean, C; Merk, M; Merkel, J; Merkin, M; Messi, R; Metlica, F.C.D; Miglioranzi, S; Minard, M-N; Moine, G; Monteil, S; Moran, D; Morant, J; Morris, J.V; Moscicki, J; Mountain, R; Mous, I; Muheim, F; Muresan, R; Murtas, F; Muryn, B; Musy, M; Mylroie-Smith, J; Naik, P; Nakada, T; Nandakumar, R; Nardulli, J; Nawrot, A; Nedos, M; Needham, M; Neufeld, N; Neustroev, P; Nicol, M; Nicolas, L; Nies, S; Niess, V; Nikitin, N; Noor, A; Oblakowska-Mucha, A; Obraztsov, V; Oggero, S; Okhrimenko, O; Oldeman, R; Orlandea, M; Ostankov, A; Palacios, J; Palutan, M; Panman, J; Papadelis, A; Papanestis, A; Pappagallo, M; Parkes, C; Parkinson, C.J; Passaleva, G; Patel, G.D; Patel, M; Paterson, S.K; Patrick, G.N; Patrignani, C; Pauna, E; Pauna (Chiojdeanu), C; Pavel (Nicorescu), C; Pazos Alvarez, A; Pellegrino, A; Penso, G; Pepe Altarelli, M; Perazzini, S; Perego, D.L; Perez Trigo, E; Perez-Calero Yzquierdo, A; Perret, P; Pessina, G; Petrella, A; Petrolini, A; Picatoste Olloqui, E; Pie Valls, B; Piedigrossi, D; Pietrzyk, B; Pinci, D; Playfer, S; Plo Casasus, M; Poli-Lener, M; Polok, G; Poluektov, A; Polycarpo, E; Popov, D; Popovici, B; Poss, S; Potterat, C; Powell, A; Pozzi, S; du Pree, T; Pugatch, V; Puig Navarro, A; Qian, W; Rademacker, J.H; Rakotomiaramanana, B; Raniuk, I; Raven, G; Redford, S; Reece, W; dos Reis, A.C; Ricciardi, S; Riera, J; Rinnert, K; Roa Romero, D.A; Robbe, P; Rodrigues, E; Rodrigues, F; Rodriguez Cobo, C; Rodriguez Perez, P; Rogers, G.J; Romanovsky, V; Rondan Sanabria, E; Rosello, M; Rospabe, G; Rouvinet, J; Roy, L; Ruf, T; Ruiz, H; Rummel, C; Rusinov, V; Sabatino, G; Saborido Silva, J.J; Sagidova, N; Sail, P; Saitta, B; Sakhelashvili, T; Salzmann, C; Sambade Varela, A; Sannino, M; Santacesaria, R; Santinelli, R; Santovetti, E; Sapunov, M; Sarti, A; Satriano, C; Satta, A; Savidge, T; Savrie, M; Savrina, D; Schaack, P; Schiller, M; Schleich, S; Schmelling, M; Schmidt, B; Schneider, O; Schneider, T; Schopper, A; Schune, M-H; Schwemmer, R; Sciubba, A; Seco, M; Semennikov, A; Senderowska, K; Serra, N; Serrano, J; Shao, B; Shapkin, M; Shapoval, I; Shatalov, P; Shcheglov, Y; Shears, T; Shekhtman, L; Shevchenko, V; Shires, A; Sigurdsson, S; Simioni, E; Skottowe, H.P; Skwarnicki, T; Smale, N; Smith, A; Smith, A.C; Smith, N.A; Sobczak, K; Soler, F.J.P; Solomin, A; Somogy, P; Soomro, F; Souza De Paula, B; Spaan, B; Sparkes, A; Spiridenkov, E; Spradlin, P; Srednicki, A; Stagni, F; Stahl, S; Steiner, S; Steinkamp, O; Stenyakin, O; Stoica, S; Stone, S; Storaci, B; Straumann, U; Styles, N; Szczekowski, M; Szczypka, P; Szumlak, T; TJampens, S; Tarkovskiy, E; Teodorescu, E; Terrier, H; Teubert, F; Thomas, C; Thomas, E; van Tilburg, J; Tisserand, V; Tobin, M; Topp-Joergensen, S; Tran, M.T; Traynor, S; Trunk, U; Tsaregorodtsev, A; Tuning, N; Ukleja, A; Ullaland, O; Uwer, U; Vagnoni, V; Valenti, G; Van Lysebetten, A; Vazquez Gomez, R; Vazquez Regueiro, P; Vecchi, S; Velthuis, J.J; Veltri, M; Vervink, K; Viaud, B; Videau, I; Vieira, D; Vilasis-Cardona, X; Visniakov, J; Vollhardt, A; Volyanskyy, D; Voong, D; Vorobyev, A; Vorobyev, An; Voss, H; Wacker, K; Wandernoth, S; Wang, J; Ward, D.R; Webber, A.D; Websdale, D; Whitehead, M; Wiedner, D; Wiggers, L; Wilkinson, G; Williams, M.P; Williams, M; Wilson, F.F; Wishahi, J; Witek, M; Witzeling, W; Woodward, M.L; Wotton, S.A; Wyllie, K; Xie, Y; Xing, F; Yang, Z; Ybeles Smit, G; Young, R; Yushchenko, O; Zeng, M; Zhang, L; Zhang, Y; Zhelezov, A; Zverev, E

2010-01-01

The production of K_short mesons in pp collisions at a centre-of-mass energy of 0.9 TeV is studied with the LHCb detector at the Large Hadron Collider. The luminosity of the analysed sample is determined using a novel technique, involving measurements of the beam currents, sizes and positions, and is found to be 6.8 +/- 1.0 microbarn^-1. The differential prompt K_short production cross-section is measured as a function of the K_short transverse momentum and rapidity in the region 0 < pT < 1.6 GeV/c and 2.5 < y < 4.0. The data are found to be in reasonable agreement with previous measurements and generator expectations.

Relationship between Gene Body DNA Methylation and Intragenic H3K9me3 and H3K36me3 Chromatin Marks

OpenAIRE

Hahn, Maria A.; Wu, Xiwei; Li, Arthur X.; Hahn, Torsten; Pfeifer, Gerd P.

2011-01-01

To elucidate the relationship between intragenic DNA methylation and chromatin marks, we performed epigenetic profiling of chromosome 19 in human bronchial epithelial cells (HBEC) and in the colorectal cancer cell line HCT116 as well as its counterpart with double knockout of DNMT1 and DNMT3B (HCT116-DKO). Analysis of H3K36me3 profiles indicated that this intragenic mark of active genes is associated with two categories of genes: (i) genes with low CpG density and H3K9me3 in the gene body or ...

Crystallite growth kinetics of TiO{sub 2} surface modification with 9 mol% ZnO prepared by a coprecipitation process

Energy Technology Data Exchange (ETDEWEB)

Ko, Horng-Huey [Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan (China); Hsi, Chi-Shiung [Department of Materials Science and Engineering, National United University, 1 Lein-Da, Kung-Ching Li, Miao-Li 36003, Taiwan (China); Wang, Moo-Chin, E-mail: mcwang@kmu.edu.tw [Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan (China); Zhao, Xiujian, E-mail: opluse@whut.edu.cn [State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070 (China)

2014-03-05

Highlights: • TiO{sub 2} powder surface modification with 9 mol% ZnO was obtained. • Phase transformation from anatase to rutile was hindered by ZnO added. • Growth kinetic of anatase TiO{sub 2} nanocrystallites in T-9Z powders was described as: D{sub A,9}{sup 2}=2.42×10{sup 5}×exp(-39.9×10{sup 3}/RT). • Growth kinetic of rutile TiO{sub 2} nanocrystallites in T-9Z powders was described as: D{sub R,9}{sup 2}=8.49×10{sup 5}×exp(-47.6×10{sup 3}/RT) rutile TiO{sub 2}. -- Abstract: The nanocrystallite growth of TiO{sub 2} surface modification with 9 mol% ZnO prepared by a coprecipitation process has been studied. Thermogravimetric and differential thermal analysis (TG/DTA), X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and UV–VIS–NIR spectrophotometry have been utilized to characterize the TiO{sub 2} nanocrystallites surface modification with 9 mol% ZnO (denoted by T-9Z). The DTA result shows that the anatase TiO{sub 2} first formed at 533 K and the completion of anatase TiO{sub 2} crystallization occurred at 745 K for the T-9Z freeze-dried precursor powders. XRD results reveal that the anatase and rutile TiO{sub 2} coexist when the T-9Z freeze-dried precursor powders were calcined at 523–973 K for 2 h. When the T-9Z freeze-dried precursor powders were calcined at 973 K for 2 h, rutile TiO{sub 2} was the major phase, and the minor phases were anatase TiO{sub 2} and Zn{sub 2}Ti{sub 3}O{sub 8}. The phase was composed of the rutile TiO{sub 2} and Zn{sub 2}TiO{sub 4} for the T-9Z freeze-dried precursor powders after calcination at 1273 K for 2 h. The growth kinetics of TiO{sub 2} nanocrystallites in T-9Z powders were described as: D{sub A,9}{sup 2}=2.42×10{sup 5}×exp(-39.9×10{sup 3}/RT)and D{sub R,9}{sup 2}=8.49×10{sup 5}×exp(-47.6×10{sup 3}/RT) for anatase and rutile TiO{sub 2} nanocrystallites respectively. The analysis results of UV/VIS/NIR spectra reveal that the T-9Z freeze

9 CFR 381.302 - Thermal processing.

Science.gov (United States)

2010-01-01

... INSPECTION AND CERTIFICATION POULTRY PRODUCTS INSPECTION REGULATIONS Canning and Canned Products § 381.302... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Thermal processing. 381.302 Section 381.302 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE...

Validation Testing of the Nitric Acid Dissolution Step Within the K Basin Sludge Pretreatment Process

International Nuclear Information System (INIS)

AJ Schmidt; CH Delegard; KL Silvers; PR Bredt; CD Carlson; EW Hoppe; JC Hayes; DE Rinehart; SR Gano; BM Thornton

1999-01-01

The work described in this report involved comprehensive bench-scale testing of nitric acid (HNO 3 ) dissolution of actual sludge materials from the Hanford K East (KE) Basin to confirm the baseline chemical pretreatment process. In addition, process monitoring and material balance information was collected to support the development and refinement of process flow diagrams. The testing was performed by Pacific Northwest National Laboratory (PNNL)for the US Department of Energy's Office of Spent Fuel Stabilization (EM-67) and Numatec Hanford Corporation (NHC) to assist in the development of the K Basin Sludge Pretreatment Process. The baseline chemical pretreatment process for K Basin sludge is nitric acid dissolution of all particulate material passing a 1/4-in. screen. The acid-insoluble fraction (residual solids) will be stabilized (possibly by chemical leaching/rinsing and grouting), packaged, and transferred to the Hanford Environmental Restoration Disposal Facility (ERDF). The liquid fraction is to be diluted with depleted uranium for uranium criticality safety and iron nitrate for plutonium criticality safety, and neutralized with sodium hydroxide. The liquid fraction and associated precipitates are to be stored in the Hanford Tank Waste Remediation Systems (TWRS) pending vitrification. It is expected that most of the polychlorinated biphenyls (PCBs), associated with some K Basin sludges, will remain with the residual solids for ultimate disposal to ERDF. Filtration and precipitation during the neutralization step will further remove trace quantities of PCBs within the liquid fraction. The purpose of the work discussed in this report was to examine the dissolution behavior of actual KE Basin sludge materials at baseline flowsheet conditions and validate the.dissolution process step through bench-scale testing. The progress of the dissolution was evaluated by measuring the solution electrical conductivity and concentrations of key species in the dissolver

Validation Testing of the Nitric Acid Dissolution Step Within the K Basin Sludge Pretreatment Process

Energy Technology Data Exchange (ETDEWEB)

AJ Schmidt; CH Delegard; KL Silvers; PR Bredt; CD Carlson; EW Hoppe; JC Hayes; DE Rinehart; SR Gano; BM Thornton

1999-03-24

The work described in this report involved comprehensive bench-scale testing of nitric acid (HNO{sub 3}) dissolution of actual sludge materials from the Hanford K East (KE) Basin to confirm the baseline chemical pretreatment process. In addition, process monitoring and material balance information was collected to support the development and refinement of process flow diagrams. The testing was performed by Pacific Northwest National Laboratory (PNNL)for the US Department of Energy's Office of Spent Fuel Stabilization (EM-67) and Numatec Hanford Corporation (NHC) to assist in the development of the K Basin Sludge Pretreatment Process. The baseline chemical pretreatment process for K Basin sludge is nitric acid dissolution of all particulate material passing a 1/4-in. screen. The acid-insoluble fraction (residual solids) will be stabilized (possibly by chemical leaching/rinsing and grouting), packaged, and transferred to the Hanford Environmental Restoration Disposal Facility (ERDF). The liquid fraction is to be diluted with depleted uranium for uranium criticality safety and iron nitrate for plutonium criticality safety, and neutralized with sodium hydroxide. The liquid fraction and associated precipitates are to be stored in the Hanford Tank Waste Remediation Systems (TWRS) pending vitrification. It is expected that most of the polychlorinated biphenyls (PCBs), associated with some K Basin sludges, will remain with the residual solids for ultimate disposal to ERDF. Filtration and precipitation during the neutralization step will further remove trace quantities of PCBs within the liquid fraction. The purpose of the work discussed in this report was to examine the dissolution behavior of actual KE Basin sludge materials at baseline flowsheet conditions and validate the.dissolution process step through bench-scale testing. The progress of the dissolution was evaluated by measuring the solution electrical conductivity and concentrations of key species in the

Free radical formation in single crystals of 9-methyladenine X-irradiated at 10 K. An electron paramagnetic resonance and electron nuclear double resonance study

International Nuclear Information System (INIS)

Hole, E.O.; Sagstuen, E.; Nelson, W.H.

1995-01-01

Single crystals of 9-methyladenine were X-irradiated at 10 K and at 65 K and were studied using K-band EPR, ENDOR and field-swept ENDOR (FSE) techniques in the temperature range 10 K to 290 K. Three major radicals are stabilized in 9-methyladenine at 10 K. These are: MA1, the adenine anion, probably protonated at N3; MA2, the species formed by net hydrogen abstraction from the 9-methyl group; and MA3, the radical formed by net hydrogen addition to C8 of the adenine moiety. Radical MA1 decayed at about 80 K, possibly into the C2 H adduct (MA4). The other two species (MA2, MA3) were stable at room temperature. A fifth radical species was clearly present in the EPR spectra at 10 K but was not detectable by ENDOR. This species, which decayed above 200 K (possibly into MA3), remains unidentified. The radical population at room temperature is as described by previous authors. The mechanisms for radical formation in 9-methyladenine are discussed in light of the hydrogen bonding scheme and molecular stacking interactions. 32 refs., 4 figs., 2 tabs

Measurement of K/sub NN/, K/sub LL/ in p vector d → n vector X and p vector 9Be → n vector X at 800 MeV

International Nuclear Information System (INIS)

Riley, P.J.; Hollas, C.L.; Newsom, C.R.

1980-01-01

The spin transfer parameters, K/sub NN/ and K/sub LL/, have been measured in p vector d → n vector X and p vector 9 Be → n vector X at 0 0 and 800 MeV. The rather large values of K/sub LL/ demonstrate that this transfer mechanism will provide a useful source of polarized neutrons at LAMPF energies

9 CFR 590.542 - Spray process drying operations.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Spray process drying operations. 590..., Processing, and Facility Requirements § 590.542 Spray process drying operations. (a) The drying room shall be... interrupted. (1) Spray nozzles, orifices, cores, or whizzers shall be cleaned immediately after cessation of...

9 CFR 590.540 - Spray process drying facilities.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Spray process drying facilities. 590.540 Section 590.540 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF..., Processing, and Facility Requirements § 590.540 Spray process drying facilities. (a) Driers shall be of a...

Genome-wide ChIP-seq mapping and analysis of butyrate-induced H3K9 and H3K27 acetylation and epigenomic landscape alteration in bovine cells

Science.gov (United States)

Utilizing next-generation sequencing technology, combined with ChIP (Chromatin Immunoprecipitation) technology, we analyzed histone modification (acetylation) induced by butyrate and the large-scale mapping of the epigenomic landscape of normal histone H3 and acetylated histone H3K9 and H3K27. To d...

Contemporary developments in algebraic K-theory

International Nuclear Information System (INIS)

Karoubi, M.; Kuku, A.O.; Pedrini, C.

2003-01-01

The School and Conference on Algebraic K-theory which took place at ICTP July 8-26, 2002 was a follow-up to the earlier one in 1997, and like its predecessor, the 2002 meeting endeavoured to emphasise the multidisciplinary aspects of the subject. However, one special feature of the 2002 School and Conference is that the whole activity was dedicated to H. Bass, one of the founders of Algebraic K-theory, on the occasion of his seventieth birthday. The School during the first two weeks, July 8 to 19 was devoted to expository lectures meant to explore and highlight connections between K-theory and several other areas of mathematics - Algebraic Topology, Number theory, Algebraic Geometry, Representation theory, and Non-commutative Geometry. This volume, constituting the Proceedings of the School, is dedicated to H. Bass. The Proceedings of the Conference during the last week July 22 - 26, which will appear in Special issues of K-theory, is also dedicated to H. Bass. The opening contribution by M. Karoubi to this volume consists of a comprehensive survey of developments in K-theory in the last forty-five years, and covers a very broad spectrum of the subject, including Topological K-theory, Atiyah-Singer index theorem, K-theory of Banach algebras, Higher Algebraic K-theory, Cyclic Homology etc. J. Berrick's contribution on 'Algebraic K-theory and Algebraic Topology' treats the various topological constructions of Algebraic K-theory together with the underlying homotopy theory. Topics covered include the plus construction together with its various ramifications and applications, Topological Hochschild and Cyclic Homology as well as K-theory of the ring of integers. The contributions by M. Kolster titled 'K-theory and Arithmetics' includes such topics as values of zeta functions and relations to K-theory, K-theory of integers in number fields and associated conjectures, Etale cohomology, Iwasawa theory etc. A.O. Kuku's contributions on 'K-theory and Representation theory

Contemporary developments in algebraic K-theory

Energy Technology Data Exchange (ETDEWEB)

Karoubi, M [Univ. Paris (France); Kuku, A O [Abdus Salam International Centre for Theoretical Physics, Trieste (Italy); Pedrini, C [Univ. Genova (Italy)

2003-09-15

The School and Conference on Algebraic K-theory which took place at ICTP July 8-26, 2002 was a follow-up to the earlier one in 1997, and like its predecessor, the 2002 meeting endeavoured to emphasise the multidisciplinary aspects of the subject. However, one special feature of the 2002 School and Conference is that the whole activity was dedicated to H. Bass, one of the founders of Algebraic K-theory, on the occasion of his seventieth birthday. The School during the first two weeks, July 8 to 19 was devoted to expository lectures meant to explore and highlight connections between K-theory and several other areas of mathematics - Algebraic Topology, Number theory, Algebraic Geometry, Representation theory, and Non-commutative Geometry. This volume, constituting the Proceedings of the School, is dedicated to H. Bass. The Proceedings of the Conference during the last week July 22 - 26, which will appear in Special issues of K-theory, is also dedicated to H. Bass. The opening contribution by M. Karoubi to this volume consists of a comprehensive survey of developments in K-theory in the last forty-five years, and covers a very broad spectrum of the subject, including Topological K-theory, Atiyah-Singer index theorem, K-theory of Banach algebras, Higher Algebraic K-theory, Cyclic Homology etc. J. Berrick's contribution on 'Algebraic K-theory and Algebraic Topology' treats the various topological constructions of Algebraic K-theory together with the underlying homotopy theory. Topics covered include the plus construction together with its various ramifications and applications, Topological Hochschild and Cyclic Homology as well as K-theory of the ring of integers. The contributions by M. Kolster titled 'K-theory and Arithmetics' includes such topics as values of zeta functions and relations to K-theory, K-theory of integers in number fields and associated conjectures, Etale cohomology, Iwasawa theory etc. A.O. Kuku's contributions on 'K-theory and Representation theory

Discovery of potential drugs for human-infecting H7N9 virus containing R294K mutation

Directory of Open Access Journals (Sweden)

He JY

2014-12-01

Full Text Available Jiao-Yu He,1,* Cheng Li,2,* Guo Wu3 1College of Life Sciences and Key Laboratory for Bio-resources of Ministry of Education, Sichuan University, 2College of Agronomy, Sichuan Agricultural University, 3College of Life Sciences, Sichuan Normal University, Chengdu, People’s Republic of China *These authors contributed equally to this work Background: After the first epidemic wave from February through May 2013, the influenza A (H7N9 virus emerged and has followed a second epidemic wave since June 2013. As of June 27, 2014, the outbreak of H7N9 had caused 450 confirmed cases of human infection, with 165 deaths included. The case-fatality rate of all confirmed cases is about 36%, making the H7N9 virus a significant threat to people’s health. At present, neuraminidase inhibitors are the only licensed antiviral medications available to treat H7N9 infections in humans. Oseltamivir is the most commonly used inhibitor, and it is also a front-line drug for the threatening H7N9. Unfortunately, it has been reported that patients treated with oseltamivir can induce R294K (Arg294Lys substitution in the H7N9 virus, which is a rare mutation and can reduce the antiviral efficacy of inhibitors. Even worse, deaths caused by such mutation after oseltamivir treatment have already been reported, indicating that the need to find substitutive neuraminidase inhibitors for currently available drugs to treat drug-resistant H7N9 is really pressing.Materials and methods: First, the structure of H7N9 containing the R294K substitution was downloaded from the Protein Data Bank, and structural information of approved drugs was downloaded from the ZINC (ZINC Is Not Commercial database. Taking oseltamivir carboxylate as a reference drug, we then filtered these molecules through virtual screening to find out potential inhibitors targeting the mutated H7N9 virus. For further evaluation, we carried out a 14 ns molecular dynamic simulation for each H7N9–drug complex and

Development of Problem Sets for K-12 and Engineering on Pharmaceutical Particulate Systems

Science.gov (United States)

Savelski, Mariano J.; Slater, C. Stewart; Del Vecchio, Christopher A.; Kosteleski, Adrian J.; Wilson, Sarah A.

2010-01-01

Educational problem sets have been developed on structured organic particulate systems (SOPS) used in pharmaceutical technology. The sets present topics such as particle properties and powder flow and can be integrated into K-12 and college-level curricula. The materials educate students in specific areas of pharmaceutical particulate processing,…

Synthesis and structural characterization of Li{sub 3}K{sub 3}Eu{sub 7}(BO{sub 3}){sub 9}

Energy Technology Data Exchange (ETDEWEB)

Braeuchle, Sebastian; Huppertz, Hubert [Innsbruck Univ. (Austria). Inst. fuer Allgemeine, Anorganische und Theoretische Chemie; Seibald, Markus [OSRAM GmbH, Schwabmuenchen (Germany). Corporate Innovation

2017-07-01

Li{sub 3}K{sub 3}Eu{sub 7}(BO{sub 3}){sub 9} was prepared by high-temperature solid state synthesis at 900 C in a platinum crucible from lithium carbonate, potassium carbonate, boric acid, and europium(III) oxide. The title compound crystallizes in the orthorhombic space group Pca2{sub 1} (no. 29) (Z = 4). The structure was refined from single-crystal X-ray diffraction data: a = 21.126(2), b = 6.502(2), c = 17.619(2) Aa, V = 2420.1(2) Aa{sup 3}, R1 = 0.0183 and wR2 = 0.0412 for all data. The crystal structure of Li{sub 3}K{sub 3}Eu{sub 7}(BO{sub 3}){sub 9} is isotypic to Li{sub 3}K{sub 3}Y{sub 7}(BO{sub 3}){sub 9} featuring isolated BO{sub 3} units and LiO{sub 6} octahedra forming [Li{sub 3}B{sub 4}O{sub 21}] units in the ac plane, which are linked by additional BO{sub 3} units. The K{sup +} and Eu{sup 3+} cations are arranged in the cavities of the structure.

Campaign 9 of the K2 Mission: Observational Parameters, Scientific Drivers, and Community Involvement for a Simultaneous Space- and Ground-based Microlensing Survey

Science.gov (United States)

Henderson, Calen B.; Poleski, Radoslaw; Penny, Matthew; Street, Rachel A.; Bennett, David P.; Hogg, David W.; Gaudi, B. Scott; Zhu, W.; Barclay, T.; Barentsen, G.;

Processing parameters for the mechanical working of 9 Cr-1 Mo steel: processing maps approach

Energy Technology Data Exchange (ETDEWEB)

Sivaprasad, P.V.; Mannan, S.L.; Prasad, Y.V.R.K. [Indira Ghandi Centre for Atomic Research, Tamilnadu (India)

2004-12-15

Processing and instability maps using a dynamic materials model have been developed for 9Cr-1Mo steel in the temperature range 850 to 1200{sup o}C and strain rate range 0.001-100 s{sup -1} with a view to optimising its hot workability. The efficiency of power dissipation increased with increase in temperature and decrease in strain rate. The 9Cr-1Mo material exhibited two dynamic recrystallisation domains, one with a peak efficiency of 37% occurring at 950{sup o}C and 0.001 s{sup -1} and the other with a peak efficiency of 35% occurring at 1200{sup o}C and 0.1 s{sup -1}. These results are in good agreement with those found in industry. (author)

21 CFR 874.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 874.9 Section 874.9 Food and Drugs FOOD AND DRUG... Cosmetic Act (the act). The exemption from the requirement of premarket notification (section 510(k) of the...

21 CFR 870.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 870.9 Section 870.9 Food and Drugs FOOD AND DRUG... Cosmetic Act (the act). The exemption from the requirement of premarket notification (section 510(k) of the...

21 CFR 868.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 868.9 Section 868.9 Food and Drugs FOOD AND DRUG... Cosmetic Act (the act). The exemption from the requirement of premarket notification (section 510(k) of the...

Clean solutions to the incoming wafer quality impact on lithography process yield limits in a dynamic copper/low-k research and development environment

Science.gov (United States)

Lysaght, Patrick S.; Ybarra, Israel; Sax, Harry; Gupta, Gaurav; West, Michael; Doros, Theodore G.; Beach, James V.; Mello, Jim

2000-06-01

The continued growth of the semiconductor manufacturing industry has been due, in large part, to improved lithographic resolution and overlay across increasingly larger chip areas. Optical lithography continues to be the mainstream technology for the industry with extensions of optical lithography being employed to support 180 nm product and process development. While the industry momentum is behind optical extensions to 130 nm, the key challenge will be maintaining an adequate and affordable process latitude (depth of focus/exposure window) necessary for 10% post-etch critical dimension (CD) control. If the full potential of optical lithography is to be exploited, the current lithographic systems can not be compromised by incoming wafer quality. Impurity specifications of novel Low-k dielectric materials, plating solutions, chemical-mechanical planarization (CMP) slurries, and chemical vapor deposition (CVD) precursors are not well understood and more stringent control measures will be required to meet defect density targets as identified in the National Technology Roadmap for Semiconductors (NTRS). This paper identifies several specific poor quality wafer issues that have been effectively addressed as a result of the introduction of a set of flexible and reliable wafer back surface clean processes developed on the SEZ Spin-Processor 203 configured for processing of 200 mm diameter wafers. Patterned wafers have been back surface etched by means of a novel spin process contamination elimination (SpCE) technique with the wafer suspended by a dynamic nitrogen (N2) flow, device side down, via the Bernoulli effect. Figure 1 illustrates the wafer-chuck orientation within the process chamber during back side etch processing. This paper addresses a number of direct and immediate benefits to the MicraScan IIITM deep-ultraviolet (DUV) step-and-scan system at SEMATECH. These enhancements have resulted from the resolution of three significant problems: (1) back surface

KEY COMPARISON: Final report on CCQM-K9.2: Subsequent key comparison on pH determination of phosphate buffer by Harned cell measurements

Science.gov (United States)

Spitzer, Petra; Giera, Janine; Fraga, Isabel C.; Tønnes Jakobsen, Pia; Jensen, Hans D.; Hyllested, Peter; Karpov, Oleg; Kutovoy, Viatcheslav; Nakamura, Susumu; Vospelova, Alena; Zvezdina, Valentina

2008-01-01

CCQM-K9.2 was performed supplementary to the key comparison CCQM-K9 on the pH determination of a phosphate buffer with nominal pH ~ 6.9 (at 25 °C). The sample composition was very similar in both comparisons. Only the source of the starting material used for sample preparation was different. The comparison was restricted to the use of the primary method for pH (Harned cell measurement) as defined in the IUPAC Recommendations [2]. The measurement temperatures were 15 °C, 25 °C, 37 °C. CCQM-K9.2, CCQM-K.9 and the first supplementary comparison CCQM-K9.1 [5] are activities of the Electrochemical Working Group (EAWG) of the CCQM. All three comparisons were coordinated by the PTB, Germany. The Danish Primary Laboratory (DPL) successfully took part in the CCQM-K9. Meanwhile the primary set-up for pH in Denmark moved from DPL affiliated to Radiometer Medical to DFM, Denmark. The subsequent comparison allows assessing the degree of equivalence for the measurement of pH at DFM after the move. Due to the interest of other laboratories in demonstrating their progress in pH measurements on the primary level the CCQM-K9.2 supplementary comparison was extended to other participants than DFM, namely NMIJ, VNIIFTRI, INMETRO and CMI. The reported quantity for CCQM-K9.2 was not the pH of the sample but the acidity function at zero chloride molality (see chapter 12). To calculate the pH value from the acidity function it is necessary to know the ionic strength of the sample buffer solution, which was undisclosed by the coordinator. With the exception of the Czech Metrology Institute, CMI, good agreement in the determined acidity function is found between the participants. The results reported by DFM and by PTB agree within their measurement uncertainty at all measurement temperatures. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report

Mechanical property changes in porous low-k dielectric thin films during processing

Energy Technology Data Exchange (ETDEWEB)

Stan, G., E-mail: gheorghe.stan@nist.gov; Gates, R. S. [Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 (United States); Kavuri, P. [Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 (United States); Torres, J.; Michalak, D.; Ege, C.; Bielefeld, J.; King, S. W. [Logic Technology Development, Intel Corporation, Hillsboro, Oregon 97124 (United States)

2014-10-13

The design of future generations of Cu-low-k dielectric interconnects with reduced electronic crosstalk often requires engineering materials with an optimal trade off between their dielectric constant and elastic modulus. This is because the benefits associated with the reduction of the dielectric constant by increasing the porosity of materials, for example, can adversely affect their mechanical integrity during processing. By using load-dependent contact-resonance atomic force microscopy, the changes in the elastic modulus of low-k dielectric materials due to processing were accurately measured. These changes were linked to alterations sustained by the structure of low-k dielectric films during processing. A two-phase model was used for quantitative assessments of the elastic modulus changes undergone by the organosilicate skeleton of the structure of porous and pore-filled dielectrics.

Bright x-ray stainless steel K-shell source development at the National Ignition Facility

Energy Technology Data Exchange (ETDEWEB)

May, M. J.; Fournier, K. B.; Colvin, J. D.; Barrios, M. A.; Dewald, E. L.; Moody, J.; Patterson, J. R.; Schneider, M.; Widmann, K. [Lawrence Livermore National Laboratory, P.O. Box 808 L170, Livermore, California 94551 (United States); Hohenberger, M.; Regan, S. P. [Laboratory for Laser Energetics, University of Rochester, Rochester, New York 14623 (United States)

2015-06-15

High x-ray conversion efficiency (XRCE) K-shell sources are being developed for high energy density experiments for use as backlighters and for the testing of materials exposed to high x-ray fluxes and fluences. Recently, sources with high XRCE in the K-shell x-ray energy range of iron and nickel were investigated at the National Ignition Facility (NIF). The x-ray conversion efficiency in the 5–9 keV spectral range was determined to be 6.8% ± 0.3%. These targets were 4.1 mm diameter, 4 mm tall hollow epoxy tubes having a 50 μm thick wall supporting a tube of 3 to 3.5 μm thick stainless steel. The NIF laser deposited ∼460 kJ of 3ω light into the target in a 140 TW, 3.3 ns square pulse. The absolute x-ray emission of the source was measured by two calibrated Dante x-ray spectrometers. Time resolved images filtered for the Fe K-shell were recorded to follow the heating of the target. Time integrated high-resolution spectra were recorded in the K-shell range.

Excitation processes in slow K+--He, Ne, Ar, H2, N2 and Na+--He collisions

International Nuclear Information System (INIS)

Kikiani, B.I.; Gochitashvili, M.R.; Kvizhinadze, R.V.; Ankudinov, V.A.

1984-01-01

Quasimolecular features of excitation processes in K + --He, Ne, Ar, H 2 , N 2 and Na + --He collisions were investigated by measuring the cross sections for the emission of the resonance lines of potassium (lambda = 766.5 and 769.9 nm), sodium (lambda = 589 and 589.6 nm), and helium (lambda = 584 nm) atoms at ion energies in the range 0.5--10 keV. In Na + --He collisions, the resonance-line excitation functions obtained for sodium and helium atoms exhibit oscillations that are in antiphase and are due to phase interference between the quasimolecular states of the system of colliding particles. Experimental data on K + --Ar collisions are interpreted in terms of schematic correlation diagrams for molecular orbitals. The excitation mechanisms for K + --N 2 and K + --Ar have beenfound to be similar, and this leads to the conclusion that the quasimolecular model used for the ion-atom case is also valid for the ion-molecule case. It is shown that the excitation of the 4p-state of the potassium atom in the K + --Ar case is due to a Landau-Zener type of interaction in the region of the quasicrossing of (KAr) + terms. Analysis of the excitation of this state in K + --N 2 collisions also shows that the capture of an electron into the excited 4p-state of the potassium atom is due to a nonadiabatic transition in the region of quasicrossing of energy terms of the same symmetry

The new development of radiation processing in China

International Nuclear Information System (INIS)

Chen Dianhua

1998-12-01

China Isotope and Radiation Association (CIRA) investigated the status of radiation processing in China's mainland. There are 45 accelerators each with beam power over 5 kW, the total beam power is 2005 kW. There are 48 γ facilities each with designed capacity over 1.11 x 10 4 TBq (0.3 million curies) and other 75 units with designed capacity less than 1.11 x 10 4 TBq. The total loaded capacity is 4.63 x 10 5 TBq, more than one third of the designed capacity. radiation processing is mainly used in producing chemical-industrial products (as heat-shrinkable products and radiation cross-linked wire and cable) in China. Some enterprises with annual output value over a hundred million RMB have emerged. Radiation processing is also used in preservation and disinfection of food. In 1977, six kinds of hygienic standards for irradiated food were authorized. Radiation sterilization of disposable medical products, radiation desulphurization and denitration are also being developed in China

Studies project development off U.K

International Nuclear Information System (INIS)

Anon.

1992-01-01

This paper reports that capital spending on U.K. Continental Shelf (UKCS) oil and gas development in 1992-94 will reach about $36 billion, Arthur Andersen Petroleum Services (AAPS) predicts. Expenditures during the 3 year period would be about 55% more than capital spending for UKCS development in 1989-91 AAPS noted. Another industry forecast, by Grampian Regional Council, Aberdeen, estimates more than 90 new fields could be developed on the UKCS during the next 20 years

Case study: physicians develop results viewer amid Y2K commotion.

Science.gov (United States)

Wurz, J A; Manis, J L

2000-01-01

Amid a flurry of Y2K preparations, which included converting patient accounting and order processing systems for six of its eight hospitals, Advocate Health Care needed to address physician complaints that the compliant software was awkward. By partnering with physicians, information systems (IS) rapidly developed a solution that met both the need for compliance and the demand for an easy-to-use, patient-centric clinical information system. A robust, browser-based results viewer provides physician access to information from patient accounting, order processing, and several clinical ancillary systems. Advocate anticipates greater challenges as the system is promoted to other sites and clinical communities.

9th International Conference on Robotics, Vision, Signal Processing & Power Applications

CERN Document Server

Iqbal, Shahid; Teoh, Soo; Mustaffa, Mohd

2017-01-01

 The proceeding is a collection of research papers presented, at the 9th International Conference on Robotics, Vision, Signal Processing & Power Applications (ROVISP 2016), by researchers, scientists, engineers, academicians as well as industrial professionals from all around the globe to present their research results and development activities for oral or poster presentations. The topics of interest are as follows but are not limited to:   • Robotics, Control, Mechatronics and Automation • Vision, Image, and Signal Processing • Artificial Intelligence and Computer Applications • Electronic Design and Applications • Telecommunication Systems and Applications • Power System and Industrial Applications • Engineering Education.

Observation of photoelectrons of magnetic conjugate point with the rocket K-9M-54

International Nuclear Information System (INIS)

Mukai, Toshinori; Hirao, Kunio

1976-01-01

The results of the observation of photoelectrons with the rocket K-9M-54 are described. The instrument used for the observation has been reported elsewhere, and it is a low energy electron spectrometer. The angle of direction of the collimator of the detector was 30 degrees to the spin axis of the rocket. Variation of the electric potential was large. The rocket K-9M-54 took off on January 17, 1976, at six o'clock. The finally attained altitude of the rocket was about 366 km. The solar zenith angles were 106 degrees at Uchinoura and 94 degrees at the magnetic conjugate point. The UV shadow heights were 361 km at Uchinoura and 116 km at the magnetic conjugate point. The distribution of the pitch angle was observed, and was almost isotropic. The altitude variation of the integrated flux is shown in a figure, and it shows remarkable difference between the present result and the previous ones observed in day-time. The relations between energy and photoelectron flux at various altitudes were obtained. The characteristic features of the energy distribution were similar to those of the spectra observed in day-time. (Kato, T.)

Involvement of histone methyltransferase GLP in HIV-1 latency through catalysis of H3K9 dimethylation

International Nuclear Information System (INIS)

Ding, Donglin; Qu, Xiying; Li, Lin; Zhou, Xin; Liu, Sijie; Lin, Shiguan; Wang, Pengfei; Liu, Shaohui; Kong, Chuijin; Wang, Xiaohui; Liu, Lin; Zhu, Huanzhang

2013-01-01

Understanding the mechanism of HIV-1 latency is crucial to eradication of the viral reservoir in HIV-1-infected individuals. However, the role of histone methyltransferase (HMT) G9a-like protein (GLP) in HIV-1 latency is still unclear. In the present work, we established four clonal cell lines containing HIV-1 vector. We found that the integration sites of most clonal cell lines favored active gene regions. However, we also observed hypomethylation of CpG of HIV 5′LTR in all four clonal cell lines. Additionally, 5′-deoxy-5′-methylthioadenosine (MTA), a broad-spectrum histone methyltransferase inhibitor, was used to examine the role of histone methylation in HIV-1 latency. MTA was found to decrease the level of H3K9 dimethylation, causing reactivation of latent HIV-1 in C11 cells. GLP knockdown by small interfering RNA clearly induced HIV-1 LTR expression. Results suggest that GLP may play a significant role in the maintenance of HIV-1 latency by catalyzing dimethylation of H3K9. - Highlights: ► We have established an in vitro model of HIV-1 latency. ► The integration sites of most clonal cell lines favor in active gene regions. ► Hypomethylation occurs in CpG islands of HIV 5′LTR in all four clonal cell lines. ► MTA can reactivate latent HIV-1 by decreasing the level of H3K9 me2 in C11 cells. ► HMT GLP may play a significant role in the maintenance of HIV-1 latency

Final report on CCQM-K9.2 : subsequent key comparison on pH determination of phosphate buffer by Harned cell measurements

OpenAIRE

Spitzer, Petra; Giera, Janine; Fraga, Isabel Cristina Serta; Jakobsen, Pia Tonnes; Jensen, Hans D.; Hyllested, Peter; Karpov, Oleg; Kutovoy, Viatcheslav; Nakamura, Susumu; Vospelova, Alena; Zvezdina, Valentina

2008-01-01

CCQM-K9.2 was performed supplementary to the key comparison CCQM-K9 [1] on the pH determination of a phosphate buffer with nominal pH ~ 6.9 (at 25 °C). The sample composition was very similar in both comparisons. Only the source of the starting material used for sample preparation was different. The comparison was restricted to the use of the primary method for pH (Harned cell measurement) as defined in the IUPAC Recommendations [2]. The measurement temperatures were15 °C, 25 °C , 37...

K25/K27 Characterization for Demonstrating Criticality Incredibility K-25 / K-27 D and D Project

International Nuclear Information System (INIS)

Haghighi, Mahmoud H.; Howe, Kathleen E.; Chandler, John R.

2008-01-01

The K-25 and K-27 Buildings are currently undergoing decontamination and decommissioning (D and D) as part of a non-time critical removal action. When the enrichment process was shut-down, the process gas equipment and piping was purged to remove UF 6 , but the process gas system was not generally chemically treated to remove solid holdup. Generally, chemical treatment was completed it was not very effective in removing the larger deposits. Because of this, uranium deposits continue to reside throughout the process gas system. There is the potential for unreacted UF 6 to be present, as well. A key part of this project is to locate the remaining significant uranium deposits and manage them appropriately. Since some process gas components in the K-25 and K-27 Buildings contain enriched uranium holdup, it is important to ensure nuclear criticality safety during demolition or subsequent loading and transport of waste. Therefore, a major task in preparation for demolition is to demonstrate that 'criticality is incredible (CI)' in each building area. The U.S. Department of Energy (DOE) and Bechtel Jacobs Company LLC (BJC) are in agreement that proper and thorough characterization of fissile holdup material in the K-25 and K-27 process gas components is a key element in achieving and demonstrating that criticality is incredible, as well as in management of demolition wastes and collection of holdup material. While the classic project scheduling wisdom may indicate that performing all of the data quality objectives (DQO) and planning before the field work starts, the size of the K-25 / K-27 D and D Project combined with the evolving decommissioning plans and re-plans have indicated that a fresh look at what further characterization data are needed to meet the current project requirements. Performing a DQO in the middle of the project allowed a much greater degree of accuracy of the real project data needs than was possible even 22 months ago. The outcome of the DQO work

K Basin Sludge Conditioning Process Testing Project. Results from Test 4, ''Acid Digestion of Mixed-Bed Ion Exchange Resin''

International Nuclear Information System (INIS)

Pool, K.H.; Delegard, C.H.; Schmidt, A.J.; Thornton, B.M.; Silvers, K.L.

1998-06-01

Approximately 73 m 3 of heterogeneous solid material, ''sludge,'' (upper bound estimate, Packer 1997) have accumulated at the bottom of the K Basins in the 100 K Area of the Hanford Site. This sludge is a mixture of spent fuel element corrosion products, ion exchange materials (organic and inorganic), graphite-based gasket materials, iron and aluminum metal corrosion products, sand, and debris (Makenas et al. 1996, 1997). In addition, small amounts of polychlorinated biphenyls (PCBs) have been found. Ultimately, it is planned to transfer the K Basins sludge to the Hanford double shell tanks (DSTs). The Hanford Spent Nuclear Fuel (HSNF) project has conducted a number of evaluations to examine technology and processing alternatives to pretreat K Basin sludge to meet storage and disposal requirements. From these evaluations, chemical pretreatment has been selected to address criticality issues, reactivity, and the destruction or removal of PCBs before the K Basin sludge can be transferred to the DSTs. Chemical pretreatment, referred to as the K Basin sludge conditioning process, includes nitric acid dissolution of the sludge (with removal of acid insoluble solids), neutrons absorber addition, neutralization, and reprecipitation. Laboratory testing is being conducted by the Pacific Northwest National Laboratory (PNNL) to provide data necessary to develop the sludge conditioning process

Development of a 0.1 kW thermoelectric power generator for military applications

International Nuclear Information System (INIS)

Menchen, W.R.

1986-01-01

A man-portable thermoelectric power source is being developed for the U.S. Army. Initially used as a dedicated power supply for the XM-21 Chemical Agent Alarm System, the set can also meet a variety of general purpose user requirements. Development of a thermoelectric power conversion device is being undertaken by the U.S. Army LABCOM Electronics Technology and Devices Laboratory to fill a need for a generator that is silent, lightweight, multi-fueled and reliable. The 0.1 kW Power Generator is rectangular in configuration and consists of a power module, electronic control assembly and fuel delivery system housed within a tubular structural frame. The generator operates on military fuels ranging from kerosene to diesel oil. Multi-fuel capability is achieved using an ultrasonic atomizer and regenerative burner developed specifically for this application. This paper provides the first public presentation of results achieved during the Advanced Development Phase of the 0.1 kW Power Generator. The development process is briefly traced with emphasis on a description of the system and test results obtained to date

Role of miRNA-9 in Brain Development

Directory of Open Access Journals (Sweden)

Balachandar Radhakrishnan

2016-01-01

Full Text Available MicroRNAs (miRNAs are a class of small regulatory RNAs involved in gene regulation. The regulation is effected by either translational inhibition or transcriptional silencing. In vertebrates, the importance of miRNA in development was discovered from mice and zebrafish dicer knockouts. The miRNA-9 (miR-9 is one of the most highly expressed miRNAs in the early and adult vertebrate brain. It has diverse functions within the developing vertebrate brain. In this article, the role of miR-9 in the developing forebrain (telencephalon and diencephalon, midbrain, hindbrain, and spinal cord of vertebrate species is highlighted. In the forebrain, miR-9 is necessary for the proper development of dorsoventral telencephalon by targeting marker genes expressed in the telencephalon. It regulates proliferation in telencephalon by regulating Foxg1, Pax6, Gsh2 , and Meis2 genes. The feedback loop regulation between miR-9 and Nr2e1/Tlx helps in neuronal migration and differentiation. Targeting Foxp1 and Foxp2 , and Map1b by miR-9 regulates the radial migration of neurons and axonal development. In the organizers, miR-9 is inversely regulated by hairy1 and Fgf8 to maintain zona limitans interthalamica and midbrain-hindbrain boundary (MHB. It maintains the MHB by inhibiting Fgf signaling genes and is involved in the neurogenesis of the midbrain-hindbrain by regulating Her genes. In the hindbrain, miR-9 modulates progenitor proliferation and differentiation by regulating Her genes and Elav3. In the spinal cord, miR-9 modulates the regulation of Foxp1 and Onecut1 for motor neuron development. In the forebrain, midbrain, and hindbrain, miR-9 is necessary for proper neuronal progenitor maintenance, neurogenesis, and differentiation. In vertebrate brain development, miR-9 is involved in regulating several region-specific genes in a spatiotemporal pattern.

Structure determination of K2ZnBr4 at 291 and 144 K

International Nuclear Information System (INIS)

Fabry, J.; Breczewski, T.; Zuniga, F.J.; Arnaiz, A.R.

1993-01-01

The room-temperature phase of K 2 ZnBr 4 is isomorphous with Sr 2 GeS 4 (P2 1 /m) while the low-temperature structure (P2 1 ) is slightly distorted [the phase transition occurs at 155 K]. Both structures contain highly deformed tetrahedral [ZnBr 4 ] 2- molecules with Br(3)-Zn-Br(3') angles of 103.06(5) and 102.49(9) at 291 and 144 K, respectively. This distortion is caused by the repulsion of Br atoms whose distance 3.712(1) and 3.661(2) A at 291 and 144 K, respectively, is below the Br-Br van der Waals distance (3.9 A). The phase transition is accompanied by minor shifts of cations and [ZnBr 4 ] 2- tetrahedra which are simultaneously rotated about a small angle. Below the phase transition point an inversion twin develops whose twin-fraction parameter was refined to 0.459(65). (orig.)

9 CFR 590.546 - Albumen flake process drying facilities.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Albumen flake process drying facilities. 590.546 Section 590.546 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... INSPECTION ACT) Sanitary, Processing, and Facility Requirements § 590.546 Albumen flake process drying...

Identification and characterization of the Spodoptera Su(var) 3-9 histone H3K9 trimethyltransferase and its effect in AcMNPV infection.

Science.gov (United States)

Li, Binbin; Li, Sisi; Yin, Juan; Zhong, Jiang

2013-01-01

Histone H3-lysine(9) (H3K9) trimethyltransferase gene Su(var) 3-9 was cloned and identified in three Spodoptera insects, Spodopterafrugiperda (S. frugiperda), S. exigua and S. litura. Sequence analysis showed that Spodoptera Su(var) 3-9 is highly conserved evolutionarily. Su(var) 3-9 protein was found to be localized in the nucleus in Sf9 cells, and interact with histone H3, and the heterochromatin protein 1a (HP1a) and HP1b. A dose-dependent enzymatic activity was found at both 27 °C and 37 °C in vitro, with higher activity at 27 °C. Addition of specific inhibitor chaetocin resulted in decreased histone methylation level and host chromatin relaxation. In contrast, overexpression of Su(var) 3-9 caused increased histone methylation level and cellular genome compaction. In AcMNV-infected Sf9 cells, the transcription of Su(var) 3-9 increased at late time of infection, although the mRNA levels of most cellular genes decreased. Pre-treatment of Sf9 cells with chaetocin speeded up viral DNA replication, and increased the transcription level of a variety of virus genes, whereas in Sf9 cells pre-transformed with Su(var) 3-9 expression vector, viral DNA replication slow down slightly. These findings suggest that Su(var) 3-9 might participate in the viral genes expression an genome replication repression during AcMNPV infection. It provided a new insight for the understanding virus-host interaction mechanism.

Search for exotic barton resonances in the final states of K/sup -/p and K/sup -/d interactions at 2. 9 GeV/c. [Cross section upper limits

Energy Technology Data Exchange (ETDEWEB)

Staab, R.W.

1975-11-01

The reactions K/sup -/p ..-->.. ..xi../sup 0/..pi../sup -/..pi../sup +/K/sup 0/ in hydrogen and K/sup -/n ..-->.. ..xi../sup -/..pi../sup -/K/sup +/ in deuterium were analyzed for isotopic spin-3/2 exotic cascade resonance production at a K/sup -/ beam momentum of 2.9 GeV/c. In addition, the strangeness +1 baryons (Z*'s) were searched for in the reaction K/sup -/d ..-->.. ..xi../sup -/Z* at the same beam momentum. The three particle states under consideration lie at the corners of an exotic decuplet. The data came from the Brandeis-Maryland-Syracuse-Tufts collaboration involving two experiments at the 31'' bubble chamber at Brookhaven National Laboratory. Each involved 10/sup 6/ pictures with a 30 event/..mu..barn exposure in hydrogen and a 17 event/..mu..barn exposure in deuterium.

Creep characterization of type 316LN and HT-9 stainless steels by the K-R creep damage model

International Nuclear Information System (INIS)

Kim, Woo Gon; Kim, Sung Ho; Ryu, Woo Seog

2001-01-01

The Kachanov and Rabotnov (K-R) creep damage model was interpreted and applied to type 316LN and HT-9 stainless steels. Seven creep constants of the model, A, B, k, m, λ, γ, and q were determined for type 316LN stainless steel. In order to quantify a damage parameter, the cavity was interruptedly traced during creep for measuring cavity area to be reflected into the damage equation. For type 316LN stainless steel, λ=ε R /ε * and λ f =ε/ε R were 3.1 and increased with creep strain. The creep curve with λ=3.1 depicted well the experimental data to the full lifetime and its damage curve showed a good agreement when γ=24. However for the HT-9 stainless steel, the values of λ and λ f were different as λ=6.2 and λ f =8.5, and their K-R creep curves did not agree with the experimental data. This mismatch in the HT-9 steel was due to the ductile fracture by softening of materials rather than the brittle fracture by cavity growth. The differences of the values in the above steels were attributed to creep ductilities at the secondary and the tertiary creep stages

Development and use of 60 kV, and 150 kV floating deck modulators for high voltage protection of multi-megawatt ion beam accelerators

International Nuclear Information System (INIS)

Barber, G.C.; Ponte, N.S.; Schilling, G.

1977-01-01

Extraction currents of 60 A at 40 kV have been produced by utilizing a 60 kV floating deck modulator interfaced to a high voltage power supply. The modulator is operated in a series mode to repetitively pulse power to the ion beam accelerator. Current monitoring and other protective circuits provide interrupt commands to the series switch tube when faults occur. The constant current characteristics of the water cooled tetrode and the rapid response of the protective circuits effectively limit the fault energy to the ion source. Three of the 60 kV decks have been modified and stacked in a series configuration to supply 150 kV, 50 A pulses. This system supplies power for development of higher-energy multi-grid sources. In this system attention has been focused on forced voltage sharing of the three decks and on protective circuits for fault conditions. All control signal processing and conditioning is performed at ground level. Fiber optic links are used to interface with the high potential associated with the floating decks. A shunt modulator incorporated with this system provides regulation of the voltage to the ion source gradient grid. Future modulator development includes a system to deliver 100 A at 80 kV

A role for repressive complexes and H3K9 di-methylation in PRDM5-associated brittle cornea syndrome

DEFF Research Database (Denmark)

Porter, Louise F; Galli, Giorgio G; Williamson, Sally

2015-01-01

skin fibroblasts and retinal tissue from BCS2 patients, to elucidate the epigenetic role of PRDM5 and mechanisms of its dysregulation in disease.First we report abnormal retinal vascular morphology in the eyes of two cousins with BCS2 (PRDM5 Δ exons 9-14) using immunohistochemistry, and mine data from......, and dysregulated H3K9 di-methylation in skin fibroblasts of three patients (p.Arg590*, p.Glu134* and Δ exons 9-14) by western blotting. These findings suggest that defective interaction of PRDM5 with repressive complexes, and dysregulation of H3K9 di-methylation, play a role in PRDM5-associated disease.......Type 2 brittle cornea syndrome (BCS2) is an inherited connective tissue disease with a devastating ocular phenotype caused by mutations in the transcription factor PRDM5 hypothesised to exert epigenetic effects through histone and DNA methylation. Here we investigate clinical samples, including...

Sodium phenylbutyrate abrogates African swine fever virus replication by disrupting the virus-induced hypoacetylation status of histone H3K9/K14.

Science.gov (United States)

Frouco, Gonçalo; Freitas, Ferdinando B; Martins, Carlos; Ferreira, Fernando

2017-10-15

African swine fever virus (ASFV) causes a highly lethal disease in swine for which neither a vaccine nor treatment are available. Recently, a new class of drugs that inhibit histone deacetylases enzymes (HDACs) has received an increasing interest as antiviral agents. Considering studies by others showing that valproic acid, an HDAC inhibitor (HDACi), blocks the replication of enveloped viruses and that ASFV regulates the epigenetic status of the host cell by promoting heterochromatinization and recruitment of class I HDACs to viral cytoplasmic factories, the antiviral activity of four HDACi against ASFV was evaluated in this study. Results showed that the sodium phenylbutyrate fully abrogates the ASFV replication, whereas the valproic acid leads to a significant reduction of viral progeny at 48h post-infection (-73.9%, p=0.046), as the two pan-HDAC inhibitors tested (Trichostatin A: -82.2%, p=0.043; Vorinostat: 73.9%, p=0.043). Further evaluation showed that protective effects of NaPB are dose-dependent, interfering with the expression of late viral genes and reversing the ASFV-induced histone H3 lysine 9 and 14 (H3K9K14) hypoacetylation status, compatible to an open chromatin state and possibly enabling the expression of host genes non-beneficial to infection progression. Additionally, a synergic antiviral effect was detected when NaPB is combined with an ASFV-topoisomerase II poison (Enrofloxacin). Altogether, our results strongly suggest that cellular HDACs are involved in the establishment of ASFV infection and emphasize that further in vivo studies are needed to better understand the antiviral activity of HDAC inhibitors. Copyright © 2017 Elsevier B.V. All rights reserved.

Observation of solar HLy-α by K-9M-59 rocket

International Nuclear Information System (INIS)

Watanabe, Norihiko; Tono, Ichiro; Koshio, Takafumi.

1978-01-01

The purpose of the observation is to measure the illumination intensity of solar HLy-α (1216 A) and the altitude distribution of O 2 density in earth atmosphere. Since solar HLy-α is strongly absorbed by O 2 in the earth atmosphere, the extinction of HLy-α depends on the altitude distribution of O 2 density. The K-9M-59 rocket was launched on September 3, 1977. The HLy-α detector was a narrow-band ion chamber filled with NO gas. The result showed that the solar HLy-α irradiance at the rocket altitude of 90 km was 3.5 x 10 11 photons/cm 2 s and the altitude distribution of O 2 density was obtained. (Yoshimori, M.)

Off-gas treatment system Process Experimental Pilot Plant (PREPP) k-t evaluation

International Nuclear Information System (INIS)

Hedahl, T.G.; Cargo, C.H.; Ayers, A.L.

1982-06-01

The scope of work for this task involves a systems' evaluation, using the Kepner-Tregoe (K-T) decision analysis methodology, of off-gas treatment alternatives for a Process Experimental Pilot Plant (PREPP). Two basic systems were evaluated: (1) a wet treatment system using a quencher and scrubber system; and (2) a dry treatment system using a spray dryer and baghouse arrangement. Both systems would neutralize acidic off-gases (HCL and SO 2 ) and remove radioactive particulates prior to release to the environment. The K-T analysis results provided a numerical comparison of the two basic off-gas treatments systems for PREPP. The overall ratings for the two systems differ by only 7%. The closeness of the evaluation indicates that either system is capable of treating the off-gases from PREPP. Based on the analysis, the wet treatment system design is slightly more favorable for PREPP. Technology development, expected operability, total costs, and safety aspects were determined to be more advantageous for the wet system design. Support technology was the only major category that appears less favorable for using the wet off-gas system for PREPP. When considering the two criteria considered most important for PREPP (capital cost and major accident prevention - both rated 10), the wet treatment system received maximum ratings. Space constraints placed on the design by the existing TAN-607 building configuration also are more easily met by the wet system design. Lastly, the level of development for the wet system indicates more applicable experience for nuclear waste processing

Simultaneous lidar observation of peculiar sporadic K and Na layers at São José dos Campos (23.1°S, 45.9°W), Brazil

Science.gov (United States)

Jiao, Jing; Yang, Guotao; Cheng, Xuewu; Liu, Zhengkuan; Wang, Jihong; Yan, Zhenzhong; Wang, Chi; Batista, Paulo; Pimenta, Alexandre; Andrioli, Vânia; Denardini, C. M.

2018-04-01

We present the first simultaneous observation of mesopause sodium (Na) and potassium (K) layer by a lidar which has Na and K channel simultaneously at the South Hemisphere site, São José dos Campos (23.1°S, 45.9°W). Measurements reported here were conducted on two nights with 3.5 and 8 h of observations in November 2016. On 20 November 2016, sporadic layers in both Na and K layer occurred above 100 km, and the higher layers corresponded well with sporadic E (Es) layer. And the density of Na at 100-105 km is higher than that at main layer around 90 km, but K density around 100 km is at least 3 times smaller compared with the K density around 90 km for the similar period. On 21 November 2016, both sporadic layers occurred in main layer height with obvious descending variations with time, which seems like tidal induced. Notably, the peak K/Na ratio slowly increased with time. And Na layer and K layer showed different processes along with time with K density reaching its maximum 1 h later than that of Na. Correlations of Na/K density, Es, and winds were also discussed.

New 5 kW free-piston Stirling space convertor developments

Science.gov (United States)

Brandhorst, Henry W., Jr.; Chapman, Peter A., Jr.

2008-07-01

The NASA Vision for Exploration of the moon may someday require a nuclear reactor coupled with a free-piston Stirling convertor at a power level of 30-40 kW. In the 1990s, Mechanical Technology Inc.'s Stirling Engine Systems Division (some of whose Stirling personnel are now at Foster-Miller, Inc.) developed a 25 kW free-piston Stirling Space Power Demonstrator Engine under the SP-100 program. This system consisted of two 12.5 kW engines connected at their hot ends and mounted in tandem to cancel vibration. Recently, NASA and DoE have been developing dual 55 and 80 W Stirling convertor systems for potential use with radioisotope heat sources. Total test times of all convertors in this effort exceed 120,000 h. Recently, NASA began a new project with Auburn University to develop a 5 kW, single convertor for potential use in a lunar surface reactor power system. Goals of this development program include a specific power in excess of 140 W/kg at the convertor level, lifetime in excess of five years and a control system that will safely manage the convertors in case of an emergency. Auburn University awarded a subcontract to Foster-Miller, Inc. to undertake development of the 5 kW Stirling convertor assembly. The characteristics of the design along with progress in developing the system will be described.

A Study on the construction of 22.9 kV distribution test line of KEPCO

Energy Technology Data Exchange (ETDEWEB)

Yang, Hi Kyun; Choi, Hung Sik; Hwang, Si Dole; Jung, Young Ho [Korea Electric Power Corp. (KEPCO), Taejon (Korea, Republic of). Research Center; Kim, Dong Hwan; Choi, Chang Huk [Korea Power Engineering Company and Architecture Engineers (Korea, Republic of)

1995-12-31

In order to enhance the reliability of power supply and the quality of electricity, a study on the construction of 22.9 kV distribution test line was performed. The main objective of this study was to establish a construction plan and a basic design to perform the construction and the detailed design of the test line effectively. (author). 21 refs., 45 figs.

Identification and Characterization of the Spodoptera Su(var) 3-9 Histone H3K9 trimethyltransferase and Its Effect in AcMNPV Infection

Science.gov (United States)

Li, Binbin; Li, Sisi; Yin, Juan; Zhong, Jiang

2013-01-01

Histone H3-lysine9 (H3K9) trimethyltransferase gene Su(var) 3-9 was cloned and identified in three Spodoptera insects, Spodoptera frugiperda ( S . frugiperda ), S . exigua and S . litura . Sequence analysis showed that Spodoptera Su(var) 3-9 is highly conserved evolutionarily. Su(var) 3-9 protein was found to be localized in the nucleus in Sf9 cells, and interact with histone H3, and the heterochromatin protein 1a (HP1a) and HP1b. A dose-dependent enzymatic activity was found at both 27 °C and 37 °C in vitro, with higher activity at 27 °C. Addition of specific inhibitor chaetocin resulted in decreased histone methylation level and host chromatin relaxation. In contrast, overexpression of Su(var) 3-9 caused increased histone methylation level and cellular genome compaction. In AcMNV-infected Sf9 cells, the transcription of Su(var) 3-9 increased at late time of infection, although the mRNA levels of most cellular genes decreased. Pre-treatment of Sf9 cells with chaetocin speeded up viral DNA replication, and increased the transcription level of a variety of virus genes, whereas in Sf9 cells pre-transformed with Su(var) 3-9 expression vector, viral DNA replication slow down slightly. These findings suggest that Su(var) 3-9 might participate in the viral genes expression an genome replication repression during AcMNPV infection. It provided a new insight for the understanding virus–host interaction mechanism. PMID:23894480

Antigenic variation of TprK facilitates development of secondary syphilis.

Science.gov (United States)

Reid, Tara B; Molini, Barbara J; Fernandez, Mark C; Lukehart, Sheila A

2014-12-01

Although primary syphilis lesions heal spontaneously, the infection is chronic, with subsequent clinical stages. Healing of the primary chancre occurs as antibodies against outer membrane antigens facilitate opsonophagocytosis of the bacteria by activated macrophages. TprK is an outer membrane protein that undergoes antigenic variation at 7 variable regions, and variants are selected by immune pressure. We hypothesized that individual TprK variants escape immune clearance and seed new disseminated lesions to cause secondary syphilis. As in human syphilis, infected rabbits may develop disseminated secondary skin lesions. This study explores the nature of secondary syphilis, specifically, the contribution of antigenic variation to the development of secondary lesions. Our data from the rabbit model show that the odds of secondary lesions containing predominately TprK variant treponemes is 3.3 times higher than the odds of finding TprK variants in disseminated primary lesions (odds ratio [OR] = 3.3 [95% confidence interval {CI}, 0.98 to 11.0]; P = 0.055) and that 96% of TprK variant secondary lesions are likely seeded by single treponemes. Analysis of antibody responses demonstrates significantly higher antibody titers to tprK variable region sequences found in the inoculum compared to reactivity to tprK variant sequences found in newly arising secondary lesions. This suggests that tprK variants escape the initial immune response raised against the V regions expressed in the inoculum. These data further support a role for TprK in immune evasion and suggest that the ability of TprK variants to persist despite a robust immune response is instrumental in the development of later stages of syphilis. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Comparing single- and dual-process models of memory development.

Science.gov (United States)

Hayes, Brett K; Dunn, John C; Joubert, Amy; Taylor, Robert

2017-11-01

This experiment examined single-process and dual-process accounts of the development of visual recognition memory. The participants, 6-7-year-olds, 9-10-year-olds and adults, were presented with a list of pictures which they encoded under shallow or deep conditions. They then made recognition and confidence judgments about a list containing old and new items. We replicated the main trends reported by Ghetti and Angelini () in that recognition hit rates increased from 6 to 9 years of age, with larger age changes following deep than shallow encoding. Formal versions of the dual-process high threshold signal detection model and several single-process models (equal variance signal detection, unequal variance signal detection, mixture signal detection) were fit to the developmental data. The unequal variance and mixture signal detection models gave a better account of the data than either of the other models. A state-trace analysis found evidence for only one underlying memory process across the age range tested. These results suggest that single-process memory models based on memory strength are a viable alternative to dual-process models for explaining memory development. © 2016 John Wiley & Sons Ltd.

100-K Target Analyte List Development for Soil

Energy Technology Data Exchange (ETDEWEB)

Ovink, R.

2012-09-18

This report documents the process used to identify source area target analytes in support of the 100-K Area remedial investigation/feasibility study (RI/FS) addendum to the Integrated 100 Area Remedial Investigation/Feasibility Study Work Plan (DOE/RL-2008-46, Rev. 0).

Towards a single empirical correlation to predict kLa across scales and processes

DEFF Research Database (Denmark)

Quintanilla Hernandez, Daniela Alejandra; Gernaey, Krist; Albæk, Mads O.

Mathematical models are increasingly used in fermentation. Nevertheless, one of the major limitations of these models is that the parameters they include are process specific, e.g. the volumetric mass transfer coefficient (kLa). Oxygen transfer was studied in order to establish a single equation...... different calculations of the average shear rate. The experimental kLa value was determined with the direct method; however, eight variations of its calculation were evaluated. Several simple correlations were fitted to the measured kLa data. The standard empirical equation was found to be best...... scales using on ‐ line viscosity measurements. A single correlation for all processes and all scales could not be established...

Forecasting electricity spot market prices with a k-factor GIGARCH process

International Nuclear Information System (INIS)

Diongue, Abdou Ka; Guegan, Dominique; Vignal, Bertrand

2009-01-01

In this article, we investigate conditional mean and conditional variance forecasts using a dynamic model following a k-factor GIGARCH process. Particularly, we provide the analytical expression of the conditional variance of the prediction error. We apply this method to the German electricity price market for the period August 15, 2000-December 31, 2002 and we test spot prices forecasts until one-month ahead forecast. The forecasting performance of the model is compared with a SARIMA-GARCH benchmark model using the year 2003 as the out-of-sample. The proposed model outperforms clearly the benchmark model. We conclude that the k-factor GIGARCH process is a suitable tool to forecast spot prices, using the classical RMSE criteria. (author)

ChIP-seq analysis of histone H3K9 trimethylation in peripheral blood mononuclear cells of membranous nephropathy patients

Energy Technology Data Exchange (ETDEWEB)

Sui, W.G. [Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department, 181st Hospital, Guilin, Guangxi (China); He, H.Y. [The Life Science College, Guangxi Normal University, Guilin, Guangxi (China); Yan, Q.; Chen, J.J. [Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department, 181st Hospital, Guilin, Guangxi (China); Zhang, R.H. [The Life Science College, Guangxi Normal University, Guilin, Guangxi (China); Dai, Y. [Clinical Medical Research Center, The Second Clinical Medical College, Shenzhen People’s Hospital, Jinan University, Shenzhen, Guangdong (China)

2013-12-12

Membranous nephropathy (MN), characterized by the presence of diffuse thickening of the glomerular basement membrane and subepithelial in situ immune complex disposition, is the most common cause of idiopathic nephrotic syndrome in adults, with an incidence of 5-10 per million per year. A number of studies have confirmed the relevance of several experimental insights to the pathogenesis of human MN, but the specific biomarkers of MN have not been fully elucidated. As a result, our knowledge of the alterations in histone methylation in MN is unclear. We used chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze the variations in a methylated histone (H3K9me3) in peripheral blood mononuclear cells from 10 MN patients and 10 healthy subjects. There were 108 genes with significantly different expression in the MN patients compared with the normal controls. In MN patients, significantly increased activity was seen in 75 H3K9me3 genes, and decreased activity was seen in 33, compared with healthy subjects. Five positive genes, DiGeorge syndrome critical region gene 6 (DGCR6), sorting nexin 16 (SNX16), contactin 4 (CNTN4), baculoviral IAP repeat containing 3 (BIRC3), and baculoviral IAP repeat containing 2 (BIRC2), were selected and quantified. There were alterations of H3K9me3 in MN patients. These may be candidates to help explain pathogenesis in MN patients. Such novel findings show that H3K9me3 may be a potential biomarker or promising target for epigenetic-based MN therapies.

Speech Processing.

Science.gov (United States)

1983-05-01

The VDE system developed had the capability of recognizing up to 248 separate words in syntactic structures. 4 The two systems described are isolated...AND SPEAKER RECOGNITION by M.J.Hunt 5 ASSESSMENT OF SPEECH SYSTEMS ’ ..- * . by R.K.Moore 6 A SURVEY OF CURRENT EQUIPMENT AND RESEARCH’ by J.S.Bridle...TECHNOLOGY IN NAVY TRAINING SYSTEMS by R.Breaux, M.Blind and R.Lynchard 10 9 I-I GENERAL REVIEW OF MILITARY APPLICATIONS OF VOICE PROCESSING DR. BRUNO

Degradation of direct yellow 9 by electro-Fenton: process study and optimization and, monitoring of treated water toxicity using catalase.

Science.gov (United States)

Kourdali, Sidali; Badis, Abdelmalek; Boucherit, Ahmed

2014-12-01

The present study was undertaken to investigate the degradation and removal of direct yellow 9 (DY9) by the electro-Fenton (EF) process in batch reactor using iron and stainless steel electrodes. DY9 removal decreased with the increase in pH (3 to 8) and increased with the increase in current intensity (0.05 to 0.2A) and [H2O2] (0 to 0.5gL(-1), but not with high doses which led to low rates of DY9 removal and OH(∙) uptake). The regression quadratic models describing DY9 degradation yield "R (percent)" and electrical energy consumption "EEC (kWhkg(-1))" were validated by the analysis of variance (ANOVA) and were both noted to fit well with the experimental data. The R(2) correlation coefficients (0.995, 0.978), those adjusted coefficients (0.986, 0.939), and F values (110.7, 24.9) obtained for the responses validated the efficiency of model. The results revealed that among several other parameters, EEC depended essentially on the degradation yield. The eco-toxicity tests showed a positive correlation between catalase activity and DY9 concentration, and catalase could be qualitatively identified to assess the effect of dye and its by-products generated during the EF process. Copyright © 2014 Elsevier Inc. All rights reserved.

Lessons-Learned from D and D Activities at the Five Gaseous Diffusion Buildings (K-25, K- 27, K-29, K-31 and K-33) East Tennessee Technology Park, Oak Ridge, TN - 13574

International Nuclear Information System (INIS)

Kopotic, James D.; Ferri, Mark S.; Buttram, Claude

2013-01-01

The East Tennessee Technology Park (ETTP) is the site of five former gaseous diffusion plant (GDP) process buildings that were used to enrich uranium from 1945 to 1985. The process equipment in the original two buildings (K-25 and K-27) was used for the production of highly enriched uranium (HEU), while that in the three later buildings (K-29, K-31 and K-33) produced low enriched uranium (LEU). Equipment was contaminated primarily with uranium and to a lesser extent technetium (Tc). Decommissioning of the GDP process buildings has presented several unique challenges and produced many lessons-learned. Among these is the importance of good, up-front characterization in developing the best demolition approach. Also, chemical cleaning of process gas equipment and piping (PGE) prior to shutdown should be considered to minimize the amount of hold-up material that must be removed by demolition crews. Another lesson learned is to maintain shutdown buildings in a dry state to minimize structural degradation which can significantly complicate characterization, deactivation and demolition efforts. Perhaps the most important lesson learned is that decommissioning GDP process buildings is first and foremost a waste logistics challenge. Innovative solutions are required to effectively manage the sheer volume of waste generated from decontamination and demolition (D and D) of these enormous facilities. Finally, close coordination with Security is mandatory to effectively manage Special Nuclear Material (SNM) and classified equipment issues. (authors)

Lessons-Learned from D and D Activities at the Five Gaseous Diffusion Buildings (K-25, K- 27, K-29, K-31 and K-33) East Tennessee Technology Park, Oak Ridge, TN - 13574

Energy Technology Data Exchange (ETDEWEB)

Kopotic, James D. [United States Department of Energy, Oak Ridge Office, P.O. Box 2001, Oak Ridge, TN 37831 (United States); Ferri, Mark S.; Buttram, Claude [URS - CH2M Oak Ridge LLC, East Tennessee Technology Park, P. O. Box 4699, Oak Ridge, TN 37831 (United States)

2013-07-01

The East Tennessee Technology Park (ETTP) is the site of five former gaseous diffusion plant (GDP) process buildings that were used to enrich uranium from 1945 to 1985. The process equipment in the original two buildings (K-25 and K-27) was used for the production of highly enriched uranium (HEU), while that in the three later buildings (K-29, K-31 and K-33) produced low enriched uranium (LEU). Equipment was contaminated primarily with uranium and to a lesser extent technetium (Tc). Decommissioning of the GDP process buildings has presented several unique challenges and produced many lessons-learned. Among these is the importance of good, up-front characterization in developing the best demolition approach. Also, chemical cleaning of process gas equipment and piping (PGE) prior to shutdown should be considered to minimize the amount of hold-up material that must be removed by demolition crews. Another lesson learned is to maintain shutdown buildings in a dry state to minimize structural degradation which can significantly complicate characterization, deactivation and demolition efforts. Perhaps the most important lesson learned is that decommissioning GDP process buildings is first and foremost a waste logistics challenge. Innovative solutions are required to effectively manage the sheer volume of waste generated from decontamination and demolition (D and D) of these enormous facilities. Finally, close coordination with Security is mandatory to effectively manage Special Nuclear Material (SNM) and classified equipment issues. (authors)

Process Approach to Formation of the Strategy of Innovation Development of Enterprises

Directory of Open Access Journals (Sweden)

Yukhymenko Vita V.

2013-12-01

Full Text Available The article considers the process of formation of the strategy of innovation development on the basis of the process approach. The author studies and identifies the most significant cause-effect interrelations between the factors that influence efficiency of the process of formation of the strategy of innovation development of enterprises and their consequences. The article offers a process approach to building the K. Ishikawa diagram based on empirical assessment of management of business processes at railway engineering enterprises. The author’s approach would help solving multiple problems of railway engineering and also could be used in other sectors of economy.

H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication.

Science.gov (United States)

Wu, Rentian; Wang, Zhiquan; Zhang, Honglian; Gan, Haiyun; Zhang, Zhiguo

2017-01-09

DNA replication is tightly regulated to occur once and only once per cell cycle. How chromatin, the physiological substrate of DNA replication machinery, regulates DNA replication remains largely unknown. Here we show that histone H3 lysine 9 demethylase Kdm4d regulates DNA replication in eukaryotic cells. Depletion of Kdm4d results in defects in DNA replication, which can be rescued by the expression of H3K9M, a histone H3 mutant transgene that reverses the effect of Kdm4d on H3K9 methylation. Kdm4d interacts with replication proteins, and its recruitment to DNA replication origins depends on the two pre-replicative complex components (origin recognition complex [ORC] and minichromosome maintenance [MCM] complex). Depletion of Kdm4d impairs the recruitment of Cdc45, proliferating cell nuclear antigen (PCNA), and polymerase δ, but not ORC and MCM proteins. These results demonstrate a novel mechanism by which Kdm4d regulates DNA replication by reducing the H3K9me3 level to facilitate formation of pre-initiative complex. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

An investigation on 800 nm femtosecond laser ablation of K9 glass in air and vacuum

Energy Technology Data Exchange (ETDEWEB)

Xu, Shi-zhen, E-mail: xusz@uestc.edu.cn [School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu 610054 (China); Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Yao, Cai-zhen [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Dou, Hong-qiang [Department of Material Science and Engineering, Sichuan Engineering Technical College, Deyang 618000 (China); Liao, Wei, E-mail: liaowei@caep.cn [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Li, Xiao-yang; Ding, Ren-jie [School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu 610054 (China); Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Zhang, Li-juan; Liu, Hao; Yuan, Xiao-dong [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Zu, Xiao-tao [School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu 610054 (China)

2017-06-01

Highlights: • The fs laser ablation of borosilicate glass (K9) were investigated under 35 and 500 fs pulses. • At high fluence regime, the ablation rate at 35 fs in air increased to a plateau, and 500 fs in air and vacuum decreased. • The mechanisms of multiple-photon ionization and impact ionization were included. • The ablation morphologies of smooth zone and laser-induced periodic surface structures were presented and illustrated. • The ablation mechanisms of non-thermal and thermal ablation were included. - Abstract: Ablation rates of K9 glass were studied as a function of femtosecond laser fluences. The central wavelength was 800 nm, and pulse durations of 35 fs and 500 fs in air and vacuum were employed. Ablation thresholds of 0.42 J/cm{sup 2} and 2.1 J/cm{sup 2} were obtained at 35 fs and 500 fs, respectively, which were independent with the ambient conditions and depend on the incident pulse numbers due to incubation effects. The ablation rate of 35 fs pulse laser increased with the increasing of laser fluence in vacuum, while in air condition, it slowly increased to a plateau at high fluence. The ablation rate of 500 fs pulse laser showed an increase at low fluence and a slow drop of ablation rate was observed at high fluence in air and vacuum, which may due to the strong defocusing effects associated with the non-equilibrium ionization of air, and/or the shielding effects of conduction band electrons (CBEs) produced by multi-photon ionization and impact ionization in K9 glass surface. The typical ablation morphologies, e.g. smooth zone and laser-induced periodic surface structures (LIPSS) were also presented and illustrated.

Recent development status of compact 2 K GM cryocoolers

Science.gov (United States)

Bao, Q.; Xu, M. Y.; Tsuchiya, A.; Li, R.

2015-12-01

To meet the growing demand for a compact cooling solution for superconducting electronic devices, we developed a two-stage 2 K GM cryocooler and a cryostat system, which can reach 46.3 K / 2.2 K on the first and second stages under no-load conditions. Nevertheless, with several innovative technologies applied, the total length of the expander cylinder is reduced to under 70% of the smallest conventional 4 K GM cryocooler. In this paper we will present the design method, including material selection and structure design with detailed explanation, which has been confirmed by both simulation and experiment.

Small-scale CHP Plant based on a 35 kWel Hermetic Four Cylinder Stirling Engine for Biomass Fuels- Development, Technology and Operating Experiences

DEFF Research Database (Denmark)

Obernberger, I.; Carlsen, Henrik; Biedermann, F.

2003-01-01

) process and the Stirling engine process. The ORC process represents an economically interesting technology for small-scale biomass-fired combined heat and power plants in a power range between 400 and 1,500 kWel. A newly developed ORC technology with a nominal electric capacity of 1,000 kW was implemented...... in the biomass CHP plant Lienz (A) in the framework of an EU demonstration project. This plant was put in operation in February 2002. Stirling engines are a promising solution for installations with nominal electric capacities between 10 and 150 kW. A biomass CHP pilot plant based on a 35 kWel-Stirling engine...

Tunable exchange bias effect in magnetic Bi0.9Gd0.1Fe0.9Ti0.1O3 nanoparticles at temperatures up to 250K

DEFF Research Database (Denmark)

Basith, M. A.; Khan, F. A.; Ahmmad, Bashir

2015-01-01

that the strength of the exchange bias effect is tunable by the field cooling. The HEB values are also found to be dependent on the temperature. This magnetically tunable exchange bias obtained at temperatures up to 250K in Bi0.9Gd0.1Fe0.9Ti0.1O3 nanoparticles may be worthwhile for potential applications.......The exchange bias (EB) effect has been observed in magnetic Bi0.9Gd0.1Fe0.9Ti0.1O3 nanoparticles.The influence of magnetic field cooling on the exchange bias effect has also been investigated. The magnitude of the exchange bias field (HEB) increases with the cooling magnetic field, showing...

Non-perturbative QCD Effect on K-Factor of Drell-Yan Process

International Nuclear Information System (INIS)

Hou Zhaoyu; Zhi Haisu; Chen Junxiao

2006-01-01

By using a non-perturbative quark propagator with the lowest-dimensional condensate contributions from the QCD vacuum, the non-perturbative effect to K-factor of the Drell-Yan process is numerically investigated for 12 6 C- 12 6 C collision at the center-of-mass energy (s) 1/2 = 200 GeV, 630 GeV respectively. Calculated results show that the non-perturbative QCD effect has just a weak influence on K-factor in the two cases.

New photocatalytic process provides 99.9+% reduction of VOC at Superfund site

Energy Technology Data Exchange (ETDEWEB)

Anon.

1999-03-01

A new photocatalytic process, dubbed the A-I-R-2000 Process, is described. The process is said to offer marked economic advantages, while providing consistent 99.9+% reduction of volatile organic compounds (VOCs) from soil vapours and groundwater at the Stamina Mills Superfund site in North Smithfield, Rhode Island. The A-I-R-2000 process has been developed by KSE Inc., of Amherst, Massachusetts, and has been licensed exclusively worldwide to Trojan Technologies, Inc., of London, Ontario. The process consists essentially of adsorption of VOCs onto a UV light-activated proprietary catalysts, for breakdown to carbon dioxide and water, and also to hydrochloric acid and a small amount of chlorine gas when the VOCs are chlorinated. With a maximum internal operating temperature of 125 degrees F, it is a low-energy system when compared to other catalytic technologies that feature thermal catalytic equipment. 1 photo.

Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Klebsiella pneumoniae Serotypes K1 and K2.

Science.gov (United States)

Siu, L Kristopher; Tsai, Yu-Kuo; Lin, Jung-Chung; Chen, Te-Li; Fung, Chang-Phone; Chang, Feng-Yee

2016-12-01

In this study, a novel colloidal gold-based immunochromatographic strip (ICS) containing anti-Klebsiella pneumoniae capsular polysaccharide polyclonal antibodies was developed to specifically detect K. pneumoniae serotypes K1 and K2. Capsular polysaccharide K1 and K2 antigens were first used to produce polyclonal anti-K1 and anti-K2 antibodies. Reference strains with different serotypes, nontypeable K. pneumoniae strains, and other bacterial species were then used to assess the sensitivity and specificity of these test strips. The detection limit was found to be 10 5 CFU, and the ICSs were stable for 6 months when stored at room temperature. No false-positive or false-negative results were observed, and equivalent results were obtained compared to those of more conventional test methods, such as PCR or serum agglutination. In conclusion, the ICS developed here requires no technical expertise and allows for the specific, rapid, and simultaneous detection of K. pneumoniae serotypes K1 and K2. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Decontamination and decommissioning plan for processing contaminated NaK at the INEL

Energy Technology Data Exchange (ETDEWEB)

LaRue, D.M.; Dolenc, M.R.

1986-09-01

This decontamination and decommissioning (D&D) plan describes the work elements and project management plan for processing four containers of contaminated sodium/potassium (NaK) and returning the Army Reentry Vehicle Facility Site (ARVFS) to a reusable condition. The document reflects the management plan for this project before finalizing the conceptual design and preliminary prototype tests of the reaction kinetics. As a result, the safety, environmental, and accident analyses are addressed as preliminary assessments before completion at a later date. ARVFS contains an earth-covered bunker, a cylindrical test pit and metal shed, and a cable trench connecting the two items. The bunker currently stores the four containers of NaK from the meltdown of the EBR-1 Mark II core. The D&D project addressed in this plan involves processing the contaminated NaK and returning the ARVFS to potential reuse after cleanup.

Decontamination and decommissioning plan for processing contaminated NaK at the INEL

Energy Technology Data Exchange (ETDEWEB)

LaRue, D.M.; Dolenc, M.R.

1986-09-01

This decontamination and decommissioning (D D) plan describes the work elements and project management plan for processing four containers of contaminated sodium/potassium (NaK) and returning the Army Reentry Vehicle Facility Site (ARVFS) to a reusable condition. The document reflects the management plan for this project before finalizing the conceptual design and preliminary prototype tests of the reaction kinetics. As a result, the safety, environmental, and accident analyses are addressed as preliminary assessments before completion at a later date. ARVFS contains an earth-covered bunker, a cylindrical test pit and metal shed, and a cable trench connecting the two items. The bunker currently stores the four containers of NaK from the meltdown of the EBR-1 Mark II core. The D D project addressed in this plan involves processing the contaminated NaK and returning the ARVFS to potential reuse after cleanup.

Strong correlation between Jc(T, H||c) and Jc(77 K, 3 T||c) in Zr-added (Gd, Y)BaCuO coated conductors at temperatures from 77 down to 20 K and fields up to 9 T

International Nuclear Information System (INIS)

Xu, A; Delgado, L; Heydari Gharahcheshmeh, M; Khatri, N; Liu, Y; Selvamanickam, V

2015-01-01

We have conducted a critical current density J c (T, H) study over a wide temperature T from 77 down to 20 K and a magnetic field H up to 9 T on more than 50 ∼ 0.9 μm-thick REBa 2 Cu 3 O 7−δ (RE = rare earth) thin films containing different concentrations of BaZrO 3 (BZO). We found that, independent of the composition, there is a linear correlation between J c (77 K, 3 T||c) and J c (T, H||c) at T down to 20 K and H up to 9 T. Moreover, J c (77 K, 3 T||c) is also linearly correlated to J c (T, H||ab) below 40 K. We ascribed this linear correlation to the dominant pinning source of BZO nanorods, which act as a strong correlated pinning at T above ∼30 K and provide weak uncorrelated point pins at lower temperatures. Our result emphasizes that J c (77 K, 3 T||c) is a key metric for metal-organic chemical vapor deposited REBa 2 Cu 3 O 7−δ coated conductors. (fast track communication)

Technical Support Document: Development of the Advanced Energy Design Guide for K-12 Schools--30% Energy Savings

Energy Technology Data Exchange (ETDEWEB)

Pless, S.; Torcellini, P.; Long, N.

2007-09-01

This Technical Support Document describes the process and methodology for the development of the Advanced Energy Design Guide for K-12 School Buildings (K-12 AEDG), a design guidance document intended to provide recommendations for achieving 30% energy savings in K-12 Schools over levels contained in ANSI/ASHRAE/IESNA Standard 90.1-1999, Energy Standard for Buildings Except Low-Rise Residential Buildings. The 30% energy savings target is the first step toward achieving net-zero energy schools; schools that, on an annual basis, draw from outside sources less or equal energy than they generate on site from renewable energy sources.

Development of food preservation and processing techniques by radiation

International Nuclear Information System (INIS)

Byun, Myung Woo; Yook, Hong Sun; Lee, Ju Woon and others

1999-03-01

Development of food preservation and processing techniques by radiation was performed. Gamma irradiation at 2-10 kGy is considered to be an effective method to control pathogenic bacteria in species including Escherichia coli O157:H7. Gamma irradiation at 5 kGy completely eliminated pathogenic bacteria in beef. Gamma irradiation at such doses and subsequent storage at less than 4 deg C could ensure hygienic quality and prolong the microbiological shelf-life resulting from the reduction of spoilage microorganisms. Gamma irradiation on pre-rigor beef shortens the aging-period, improves tenderness and enhances the beef quality. And, a new beef processing method using gamma irradiation, such as in the low salt sausage and hygienic beef patty was developed. Safety tests of gamma-irradiated meats(beefs: 0-5 kGy; porks: 0-30 kGy) in areas such as genotoxicity, acute toxicity, four-week oral toxicity, rat hepato carcinogenesis and the anti oxidative defense system, were not affected by gamma irradiation. To pre-establish an alternative technique to the toxic fumigant, methyl bromide, which is the current quarantine measure of agricultural products for export and import, some selected agricultural products, such as chestnuts, acorns, red beans and mung beans, were subjected to a preliminary study to confirm the comparative effects of gamma irradiation and MBr fumigant on their disinfestation and quality, thereby preparing the basic data for the practical approach.Current fumigation(MBr) was perfect in its disinfecting capability, but it caused detrimental effects on the physical quality of agricultural produce. However, irradiation doses suitable for controlling pests did not induce any significant changes in the quality of the products. (author)

The development of a cryogenic integrated system with the working temperature of 100K

Science.gov (United States)

Liu, En'guang; Wu, Yi'nong; Wang, Yueming; Wen, Jiajia; Lv, Gang; Li, Chunlai; Hou, Jia; Yuan, Liyin

2016-05-01

In the infrared system, cooling down the optic components' temperature is a better choice to decrease the background radiation and maximize the sensitivity. This paper presented a 100K cryogenic optical system, for which an integrated designation of mechanical cooler, flexible thermal link and optical bench was developed. The whole infrared optic components which were assembled in a vacuum box were cooled down to 100K by two mechanical coolers. Low thermal conductivity supports and low emissivity multi-layers were used to reduce the cryogenic optical system's heat loss. The experiment results showed that in about eight hours, the temperature of the optical components reached 100K from room temperature, and the vibration from the mechanical coolers nearly have no affection to the imaging process by using of thermal links. Some experimental results of this cryogenic system will be discussed in this paper.

Comparison of food processing retention factors of 137Cs and 40K in vegetables

International Nuclear Information System (INIS)

Keiko Tagami; Shigeo Uchida

2013-01-01

Removal of radiocesium from food by processing is of great concern following the accident of TEPCO's Fukushima Daiichi Nuclear Power Plant accident. To provide more Cs removal rates, we studied the applicability of K data using edible plant samples. Values were compared for 137 Cs and 40 K of the food processing retention factors, F r (total activity in processed food/total activity in raw food), and they were found to be close to the 1:1 line and highly correlated (R = 0.96, p r ) x 100, for ten vegetable types to provide potential Cs removal rates. The average percent of K removal were as follows: dried pulses, 33; fresh pulses, 15; leafy vegetables, 52; fruit vegetables, 7; flower vegetables, 44: root crops, 18; tubers, 16; ferns, 93: mushrooms, 32; and others, 30. (author)

Process development

Energy Technology Data Exchange (ETDEWEB)

Schuegerl, K

1984-01-01

The item 'process development' comprises the production of acetonic/butonal with C. acetobylicum and the yeasting of potato waste. The target is to increase productivity by taking the following measures - optimation of media, on-line process analysis, analysis of reaction, mathematic modelling and identification of parameters, process simulation, development of a state estimator with the help of the on-line process analysis and the model, optimization and adaptive control.

Microstructural changes due to laser peening in modified 9Cr-1Mo steel subjected to creep damage at 823K and 923K in air

International Nuclear Information System (INIS)

Nakasone, Yuji; Kizuki, Yuta; Suzuki, Hayao; Minowa, Takuya

2013-01-01

The present study has investigated microstructural changes due to laser peening in modified 9Cr-1Mo steel subjected to creep. The EBSD or Electron Backscatter Diffraction studies have been made on round-bar type specimens creeping at applied stresses of 230 and 240 MPa at 823 K and 105 MPa at 923K in air. Prior to the creep tests, laser peening was applied to specimens at laser power of 8.4-22GW/cm 2 per pulse in water. Microstructural change in each specimen after its creep test was investigated by EBSD/SEM. The EBSD/SEM analyses revealed that the laser peening treatment makes creep rupture time longer and it reveals local misorientation value for rupture. (author)

K-mean clustering algorithm for processing signals from compound semiconductor detectors

International Nuclear Information System (INIS)

Tada, Tsutomu; Hitomi, Keitaro; Wu, Yan; Kim, Seong-Yun; Yamazaki, Hiromichi; Ishii, Keizo

2011-01-01

The K-mean clustering algorithm was employed for processing signal waveforms from TlBr detectors. The signal waveforms were classified based on its shape reflecting the charge collection process in the detector. The classified signal waveforms were processed individually to suppress the pulse height variation of signals due to the charge collection loss. The obtained energy resolution of a 137 Cs spectrum measured with a 0.5 mm thick TlBr detector was 1.3% FWHM by employing 500 clusters.

Development of large-capacity refrigeration at 1.8 K for the Large Hadron Collider at CERN

CERN Document Server

Lebrun, P; Claudet, G

1996-01-01

CERN, the European Laboratory for Particle Physics, is working towards the construction of the Large Hadron Collider (LHC), a high-energy, high-luminosity particle accelerator and collider [1] of 26.7 km circumference, due to start producing frontier physics, by bringing into collision intense proton and ion beams with centre-of-mass energies in the TeV-per-constituent range, at the beginning of the next century. The key technology for achieving this ambitious scientific goal at economically acceptable cost is the use of high-field superconducting magnets using Nb-Ti conductor operating in superfluid helium [2]. To maintain the some 25 km of bending and focusing magnets at their operating temperature of 1.9 K, the LHC cryogenic system will have to produce an unprecedented total refrigeration capacity of about 20 kW at 1.8 K, in eight cryogenic plants distributed around the machine circumference [3]. This has requested the undertaking of an industrial development programme, in the form of a collaboration betwe...

Photoluminescence properties of whitlockite-type Ca{sub 9}MgK(PO{sub 4}){sub 7}:Eu{sup 2+},Mn{sup 2+} phosphor

Energy Technology Data Exchange (ETDEWEB)

Guo, Ning, E-mail: guoning@usst.edu.cn [Department of Chemistry, University of Shanghai for Science and Technology, Shanghai 200093 (China); Li, Shuo; Chen, Jishen; Li, Jing; Zhao, Yuefeng; Wang, Lu; Jia, Chengzheng; Ouyang, Ruizhuo [Department of Chemistry, University of Shanghai for Science and Technology, Shanghai 200093 (China); Lü, Wei [State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (China)

2016-11-15

Novel single-phased Eu{sup 2+}/Mn{sup 2+}-coactivated whitlockite-type Ca{sub 9}MgK(PO{sub 4}){sub 7} phosphors which can emit white light upon UV light excitation, are prepared by the solid-state method, and their luminescence properties are systematically investigated via a combination of X-ray powder diffraction and spectroscopy measurements. For Eu{sup 2+}–Mn{sup 2+} codoped samples, an efficient energy transfer process can takes place and its mechanism is a resonant type via a dipole-quadrupole interaction which can be elucidated by DexterГ—Віs theoretical model. Following the principle of energy transfer, myriad luminescence colors with a large gamut from blue to purplish red and across white zone in a line in the chromaticity diagram of the CIE can be obtained by simply adjusting the concentration ratio of Eu{sup 2+} to Mn{sup 2+}. Photoluminescence spectra reveal that the white color emission is originated from the combination of two emission bands of Eu{sup 2+} and Mn{sup 2+} ions. Additionally, their CIE chromaticity coordinates and correlated color temperatures (CCT) have been calculated and discussed in detail. The luminescence suggest that whitlockite-type phosphor, Ca{sub 9}MgK(PO{sub 4}){sub 7}, co-activated with europium and manganese, is a promising single-phased white-emitting candidate for use in ultraviolet-chip-based white LEDs.

Dissolution behavior of bioactive glass ceramics with different CaO/MgO ratios in SBF-K9 and r-SBF

Directory of Open Access Journals (Sweden)

M.U. Hashmi

2014-08-01

Full Text Available In the present work, we studied dissolution behavior of three glass ceramics samples each having 34 SiO2–14.5 P2O5–1 CaF2–0.5 MgF2 (%wt and ratio of CaO/MgO varying from 11.5:1 to 1:11.5 in conventional SBF (SBF-K9 and revised SBF (r-SBF that has ionic concentration exactly equal to that of human blood plasma. For that purpose, samples were immersed in fluids for different time periods upto 25 days. Thin film XRD analysis revealed the diffusive nature of the phases on the surfaces of samples after soaking for different time periods in r-SBF. It showed the poor precipitation and small thickness of the HCAp layer on the samples as compared to that in SBF-K9, thus indicating the fitness and sensitivity of r-SBF for in-vitro characterization of samples. AAS, FTIR and EDS revealed slow bonding rate on the surfaces of the samples in r-SBF than that in SBF-K9 that showed the dependence of bond formation on the composition of the materials as well as on the physiological fluid used for in-vitro characterization. The rate of HCAp formation was slower in r-SBF due to more competitive adsorption of CO3− ions to Ca and Mg ions owing to greater amount of CO3− in r-SBF than that in SBF-K9. It shows the importance of CO3− content in the physiological fluids for the in-vitro assessment of samples. So, r-SBF is recommended to be used for assessment of samples to clearly understand their behavior in-vivo.

Keratins K2 and K10 are essential for the epidermal integrity of plantar skin.

Science.gov (United States)

Fischer, Heinz; Langbein, Lutz; Reichelt, Julia; Buchberger, Maria; Tschachler, Erwin; Eckhart, Leopold

2016-01-01

K1 and K2 are the main type II keratins in the suprabasal epidermis where each of them heterodimerizes with the type I keratin K10 to form intermediate filaments. In regions of the ears, tail, and soles of the mouse, only K2 is co-expressed with K10, suggesting that these keratins suffice to form a mechanically resilient cytoskeleton. To determine the effects of the suppression of both main keratins, K2 and K10, in the suprabasal plantar epidermis of the mouse. Krt2(-/-) Krt10(-/-) mice were generated by crossing Krt2(-/-) and Krt10(-/-) mice. Epidermal morphology of soles of hind-paws was examined macroscopically and histologically. Immunofluorescence analysis and quantitative PCR analysis were performed to analyze the expression of keratins in sole skin of wildtype and Krt2(-/-) Krt10(-/-) mice. Highly abundant proteins of the sole stratum corneum were determined by electrophoretic and chromatographic separation and subsequent mass spectrometry. K2 and K10 are the most prominent suprabasal keratins in normal mouse soles with the exception of the footpads where K1, K9 and K10 predominate. Mice lacking both K2 and K10 were viable and developed epidermal acanthosis and hyperkeratosis in inter-footpad epidermis of the soles. The expression of keratins K1, K9 and K16 was massively increased at the RNA and protein levels in the soles of Krt2(-/-) Krt10(-/-) mice. This study demonstrates that the loss of the main cytoskeletal components of plantar epidermis, i.e. K2 and K10, can be only partly compensated by the upregulation of other keratins. The thickening of the epidermis in the soles of Krt2(-/-) Krt10(-/-) mice may serve as a model for pathomechanistic aspects of palmoplantar keratoderma. Copyright © 2015. Published by Elsevier Ireland Ltd.

Dalitz Plot Analysis of the Decay B+ -> K+K+K-

Energy Technology Data Exchange (ETDEWEB)

Dvoretskii, Alexei; /SLAC /Caltech

2006-05-05

The authors perform an analysis of the three-body charmless decay B{sup {+-}} {yields} K{sup {+-}}K{sup {+-}}K{sup {-+}} using a sample of 226.0 {+-} 2.5 million B{bar B} pairs collected by the BABAR detector and measure the total branching fraction and Cp asymmetry to be {beta} = (35.2 {+-} 0.9 {+-} 1.6) x 10{sup -6} and A{sub CP} = (-1.7 {+-} 2.6 {+-} 1.5)%. They fit the Dalitz plot distribution using an isobar model and report the measured values of magnitudes and phases of the production coefficients. The decay dynamics is dominated by the K{sup +}K{sup -} S-wave, for which we perform a partial-wave analysis in the region m(K{sup +}K{sup -}) < 2 GeV/c{sup 2}. They find no evidence of CP violation for individual components of the isobar model.

Development of a 2kWe LPG fuel processor for PEFC

International Nuclear Information System (INIS)

Cipiti, F.; Pino, L.; Vita, A.; Cordaro, M.; Lagana, M.; Recupero, V.

2004-01-01

The successful development of Polymer Electrolyte Fuel Cells (PEFC's) for stationary and/or transportation purposes is strictly dependent on the choice of a proper fuel processor. This paper covers the in progress activities performed at CNR-ITAE on the development of a 2 kWequivalent hydrogen generator unit, (under testing) feed by LPG (propane). The main issues that should be satisfied by the hydrogen generator will be high fuel conversion, stable performance for repeated start-up and shut-down cycles, capability to process different hydrocarbons, etc. The actual unit, is constituted by an autothermal reactor (ATR) with a proprietary CNR/ITAE catalyst, an intermediate water gas shift (ITS) and a CO preferential oxidation (PROX) reactors containing commercial catalysts; the system includes heat exchangers, manual and automatic valves, pressure regulators and transducers, flow meters and ancillaries. External heating is supplied only during the start-up; on regime operations the global heat balance is smoothly exothermic. The main objectives of the experimental tests are: evaluation of reactors and system performance (in steady state and in transient response), identification of main operating limits of the reactors, to validate heat and mass balance. Preliminary results, for the 2 kW prototype, are presented. (author)

9 CFR 381.306 - Processing and production records.

Science.gov (United States)

2010-01-01

... INSPECTION AND CERTIFICATION POULTRY PRODUCTS INSPECTION REGULATIONS Canning and Canned Products § 381.306... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Processing and production records. 381.306 Section 381.306 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF...

44 CFR 78.9 - Planning grant approval process.

Science.gov (United States)

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood Insurance Program FLOOD MITIGATION ASSISTANCE § 78.9 Planning grant approval process. The State POC will evaluate and approve applications for Planning Grants. Funds will be provided only for the flood portion of any mitigation plan, and...

9 CFR 355.24 - Processes to be supervised.

Science.gov (United States)

2010-01-01

... INSPECTION AND CERTIFICATION CERTIFIED PRODUCTS FOR DOGS, CATS, AND OTHER CARNIVORA; INSPECTION... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Processes to be supervised. 355.24 Section 355.24 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE...

Room temperature continuous wave operation of quantum cascade laser at λ ~ 9.4 μm

Science.gov (United States)

Hou, Chuncai; Zhao, Yue; Zhang, Jinchuan; Zhai, Shenqiang; Zhuo, Ning; Liu, Junqi; Wang, Lijun; Liu, Shuman; Liu, Fengqi; Wang, Zhanguo

2018-03-01

Continuous wave (CW) operation of long wave infrared (LWIR) quantum cascade lasers (QCLs) is achieved up to a temperature of 303 K. For room temperature CW operation, the wafer with 35 stages was processed into buried heterostructure lasers. For a 2-mm-long and 10-μm-wide laser with high-reflectivity (HR) coating on the rear facet, CW output power of 45 mW at 283 K and 9 mW at 303 K is obtained. The lasing wavelength is around 9.4 μm locating in the LWIR spectrum range. Project supported by the National Key Research And Development Program (No. 2016YFB0402303), the National Natural Science Foundation of China (Nos. 61435014, 61627822, 61574136, 61774146, 61674144, 61404131), the Key Projects of Chinese Academy of Sciences (Nos. ZDRW-XH-2016-4, QYZDJ-SSW-JSC027), and the Beijing Natural Science Foundation (No. 4162060, 4172060).

A systematic process for developing and assessing accident management plans

International Nuclear Information System (INIS)

Hanson, D.J.; Blackman, H.S.; Meyer, O.R.; Ward, L.W.

1991-04-01

This document describes a four-phase approach for developing criteria recommended for use in assessing the adequacy of nuclear power plant accident management plans. Two phases of the approach have been completed and provide a prototype process that could be used to develop an accident management plan. Based on this process, a preliminary set of assessment criteria are derived. These preliminary criteria will be refined and improved when the remaining steps of the approach are completed, that is, after the prototype process is validated through application. 9 refs., 10 figs., 7 tabs

Mask compensation for process flare in 193nm very low k1 lithography

Science.gov (United States)

Lee, Jeonkyu; Lee, Taehyeong; Oh, Sangjin; Kang, Chunsoo; Kim, Jungchan; Choi, Jaeseung; Park, Chanha; Yang, Hyunjo; Yim, Donggyu; Do, Munhoe; Su, Irene; Song, Hua; Choi, Jung-Hoe; Fan, Yongfa; Wang, Anthony C.; Lee, Sung-Woo; Boone, Robert; Lucas, Kevin

2013-04-01

Traditional rule-based and model-based OPC methods only simulate in a very local area (generally less than 1um) to identify and correct for systematic optical or process problems. Despite this limitation, however, these methods have been very successful for many technology generations and have been a major reason for the industry being able to tremendously push down lithographic K1. This is also enabled by overall good across-exposure field lithographic process control which has been able to minimize longer range effects across the field. Now, however, the situation has now become more complex. The lithographic single exposure resolution limit with 1.35NA tools remains about 80nm pitch but the final wafer dimensions and final wafer pitches required in advanced technologies continue to scale down. This is putting severe strain on lithographic process and OPC CD control. Therefore, formerly less important 2nd order effects are now starting to have significant CD control impact if not corrected for. In this paper, we provide examples and discussion of how optical and chemical flare related effects are becoming more problematic, especially at the boundaries of large, dense memory arrays. We then introduce a practical correction method for these systematic effects which reuses some of the recent long range effect correcting OPC techniques developed for EUV pattern correction (such as EUV flare). We next provide analysis of the benefits of these OPC methods for chemical flare issues in 193nm lithography very low K1 lithography. Finally, we summarize our work and briefly mention possible future extensions.

Search for the decay of a B0 or B0bar meson to K*0bar K0 or K*0 K0bar

Energy Technology Data Exchange (ETDEWEB)

Aubert, B.

2006-06-27

The authors present a search for the decay of a B{sup 0} or {bar B}{sup 0} meson to a {bar K}*{sup 0} K{sup 0} or K*{sup 0} {bar K}{sup 0} final state, using a sample of approximately 232 million B{bar B} events collected with the BABAR detector at the PEP-II asymmetric energy e{sup +}e{sup -} collider at SLAC. The measured branching fraction is {Beta}(B{sup 0} {yields} {bar K}*{sup 0} K{sup 0}) + {Beta}(B{sup 0} {yields} K*{sup 0} {bar K}{sup 0}) = (0.2{sub -0.8, -0.3}{sup +0.9, +0.1}) x 10{sup -6}. They obtain the following upper limit for the branching fraction at 90% confidence level: {Beta}(B{sup 0} {yields} {bar K}*{sup 0} K{sup 0}) + {Beta}(B{sup 0} {yields} K*{sup 0} {bar K}{sup 0}) < 1.9 x 10{sup -6}. They use our result to constrain the Standard Model prediction for the deviation of the CP asymmetry in B{sup 0} {yields} {phi}K{sup 0} from sin 2{beta}.

Nucleoli in human early erythroblasts (K2, K1, K1/2 cells).

Science.gov (United States)

Smetana, K; Jirásková, I; Klamová, H

2005-01-01

Human early erythroid precursors classified according to the nuclear size were studied to provide information on nucleoli in these cells using simple cytochemical procedures for demonstration of RNA and proteins of silver-stained nucleolar organizers. K2 cells with nuclear diameter larger than 13 microm and K1 cells with nuclear diameter larger than 9 microm corresponding to proerythroblasts and macroblasts (large basophilic erythroblasts) mostly possessed large irregularly shaped nucleoli with multiple fibrillar centres representing "active nucleoli". K1/2 cells with nuclear diameter smaller than 9 microm corresponding to small basophilic erythroblasts were usually characterized by the presence of micronucleoli representing "inactive nucleolar types". On the other hand, a few K1/2 cells contained large nucleoli with multiple fibrillar centres similar to those present in K2 cells and thus appeared as "microproerythroblasts". The nucleolar asynchrony expressed by the presence of large irregularly shaped nucleoli with multiple nucleoli (active nucleoli) and ring-shaped nucleoli (resting nucleoli) in one and the same nucleus of K2 or K1 cells was not exceptional and might reflect a larger resistance of these cells to negative factors influencing the erythropoiesis. The intranucleolar translocation of silver-stained nucleolus organized regions was noted in K2 cells and might indicate the premature aging of these cells without further differentiation. More studies, however, are required in this direction.

Involvement of p38 MAPK- and JNK-modulated expression of Bcl-2 and Bax in Naja nigricollis CMS-9-induced apoptosis of human leukemia K562 cells.

Science.gov (United States)

Chen, Ying-Jung; Liu, Wen-Hsin; Kao, Pei-Hsiu; Wang, Jeh-Jeng; Chang, Long-Sen

2010-06-15

CMS-9, a phospholipase A(2) (PLA(2)) isolated from Naja nigricollis venom, induced apoptosis of human leukemia K562 cells, characterized by mitochondrial depolarization, modulation of Bcl-2 family members, cytochrome c release and activation of caspases 9 and 3. Moreover, an increase in intracellular Ca2+ concentration and the production of reactive oxygen species (ROS) was noted. Pretreatment with BAPTA-AM (Ca2+ chelator) and N-acetylcysteine (NAC, ROS scavenger) proved that Ca2+ was an upstream event in inducing ROS generation. Upon exposure to CMS-9, activation of p38 MAPK and JNK was observed in K562 cells. BAPTA-AM or NAC abrogated CMS-9-elicited p38 MAPK and JNK activation, and rescued viability of CMS-9-treated K562 cells. SB202190 (p38 MAPK inhibitor) and SP600125 (JNK inhibitor) suppressed CMS-9-induced dissipation of mitochondrial membrane potential, Bcl-2 down-regulation, Bax up-regulation and increased mitochondrial translocation of Bax. Inactivation of PLA(2) activity reduced drastically the cytotoxicity of CMS-9, and a combination of lysophosphatidylcholine and stearic acid mimicked the cytotoxic effects of CMS-9. Taken together, our data suggest that CMS-9-induced apoptosis of K562 cells is catalytic activity-dependent and is mediated through mitochondria-mediated death pathway triggered by Ca2+/ROS-evoked p38 MAPK and JNK activation. 2010 Elsevier Ltd. All rights reserved.

Trade Study for 9 kW Water Membrane Evaporator

Science.gov (United States)

Bue, Grant C.; Ungar, Gene; Stephan, Ryan

2010-01-01

Sublimators have been proposed and used in spacecraft for heat rejection. Sublimators are desirable heat rejection devices for short duration use because they can transfer large amounts of heat using little mass and are self-regulating devices. Sublimators reject heat into space by freezing water inside a porous substrate, allowing it to sublimate into vapor, and finally venting it into space. The state of the art thermal control system in orbiting spacecraft is a two loop, two fluid system. The external coolant loop typically uses a toxic single phase fluid that acquires heat from the spacecraft and rejects most of it via a radiator. The sublimator functions as a transient topper for orbiting spacecraft during day pass periods when radiator efficiency decreases. The sublimator interfaces with the internal loop through a built in heat exchanger. The internal loop fluid is non-toxic and is typically a propylene glycol and water solution with inhibitors to prevent corrosion with aluminum fins of the heat exchangers. Feedwater is supplied from a separate line to the sublimator to maintain temperature control of the cabin and vehicle hardware. Water membrane evaporators have been developed for spacecraft and spacesuits. They function similar to a sublimator but require a backpressure valve which could be actuated for this application with a simple fully open or fully closed modes. This technology would be applied to orbital thermal control (lunar or planetary). This paper details a trade study showing that evaporators would greatly reduce the consumable that is used, effectively wasted, by sublimators during start up and shut down during the topping phases of each orbit. State of the art for 9 kW sublimators reject about 870 W per kilogram of mass and 1150 W per liter of volume. If water with corrosion inhibitors is used the evaporators would be about 80% of the mass and volume of the equivalent system. The size and mass increases to about 110% if the internal fluid is

Development of a 600 kJ HTS SMES

International Nuclear Information System (INIS)

Seong, K.C.; Kim, H.J.; Kim, S.H.; Sim, K.D.; Sohn, M.H.; Lee, E.Y.; Park, S.J.; Hahn, S.Y.; Park, M.W.

2008-01-01

This paper describes an overview of development on a 600 kJ high-temperature superconducting magnetic energy storage (HTS SMES). Our final goal will be the commercialization of MJ class HTS SMES system for the increase of power quality within 5 years. Hence, for this purpose, we have developed the research and development in 3 years. The purpose of this research is to develop a pilot system, which can protect the sensitivity loads from a momentary power interruption or a voltage sag

21 CFR 864.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 864.9 Section 864.9 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY...

Development of 1 kW Stirling cryocooler using a linear compressor

International Nuclear Information System (INIS)

Ko, J; Kim, H; Hong, Y J; Yeom, H; In, S; Park, S J

2015-01-01

Cryogenic cooling systems for HTS electric power devices require a reliable and efficient high-capacity cryocooler. A Striling cryocooler with a linear compressor can be a good candidate. It has advantages of low vibration and long maintenance cycle compared with a kinematic-driven Stirling cryocooler. In this study, we developed a dual-opposed linear compressor of 12 kW electric input power with two 6 kW linear motors. Electrical performance of the fabricated linear compressor is verified by experimental measurement of thrust constant. The developed Stirling cryocooler has a gamma-type configuration. The piston and displacer are supported with a flexure spring. A slit-type heat exchanger is adopted for the cold and warm-end, and the generated heat is rejected by cooling water. In the cooling performance test, waveforms of voltage, current, displacement and pressure are obtained and their amplitude and phase difference are analysed. The developed cryocooler reaches 47.8 K within 23.4 min. with no-load. Heat load tests shows a cooling capacity of 440 W at 78.1 K with 6.45 kW of electric input power and 19.4 of % Carnot COP. (paper)

Studsvik SUPER-RAMP-II: 9x9 Project

International Nuclear Information System (INIS)

Djurle, S.

1991-12-01

EPRI participated in an international program involving ramp-testing of 9 x 9 fuel rod segments in a test reactor in Sweden. The principal objective was to establish the pellet-cladding interaction (PCI) failure threshold for this type of fuel. Several segmented fuel rods were irradiated in Dresden-2 BWR with time-averaged linear heat ratings ranging from 13 to 17 kW/m up to average burnup ranging from 26 to 34 MWd/kgU. The segments were subsequently subjected to ramp-testing in the R2 reactor at Studsvik Nuclear. The ramp-testing consisted of an initial irradiation of peak linear heat rating between 12 to 16 kW/m for about two hours, followed by a rapid increase to a power level in the range of 36 to 50 kW/m. The upper power level was maintained for twelve hours or until the fuel failure was detected. The study concluded that for 9 x 9 fuel type the PCI failure threshold is ∼38 kW/m, independent of the burnup in the range of 26 to 34 MWd/kgU. This failure threshold is about 5 kW/m greater than that of traditional 8 x 8 design. Although the significance of this difference can be argued, the interim and final fuel examinations performed in this project by destructive and nondestructive methods revealed valuable detailed information on fuel performance, which is also documented and discussed in this report

Final environmental impact statement, Beaufort Sea oil and gas development/Northstar Project. Appendix K (continued)

International Nuclear Information System (INIS)

1999-02-01

BP Exploration (Alaska) Inc. (BPXA) submitted a permit application to the US Army Engineer District, Alaska to initiate the review process for BPXA's plans to develop and produce oil and gas from the Northstar Unit. This report contains Appendices K (continued) of an Environmental Impact Statement which was undertaken to identify and evaluate the potential effects the proposed project may have on the environment

submitter Development of a 100 kW, 12.5 kV, 22 kHz and 30 kV insulated medium frequency transformer for compact and reliable medium voltage power conversion

CERN Document Server

Isler, Stephane; Aguglia, Davide; Bonnin, Xavier Abel

2017-01-01

This paper presents the critical design aspects addressed during the development of a 100 kW, 12.5 kV, 22 kHz, and 30 kV insulated medium frequency transformer used in a power converter. The transformers are used in a resonant multilevel converter topology producing HV DC voltage from a three phase 400 V AC industrial grid. The power converter is used to supply radio frequency systems in particle accelerators. Considerations about material selection, dielectric, magnetic and thermal design are discussed and experimental results on the full scale transformer and power converter are presented.

[Energy pooling collisions for K(4P) + Cs(5D) in a K-Cs mixture].

Science.gov (United States)

Aihemaiti, Pulati; Dai, Kang; Lu, Xin-hong; Shen, Yi-fan

2005-04-01

The rate coefficients for energy-pooling collisions K(4P) + Cs(5D) --> Cs(6S) + K(4D, 6S) in the K-Cs vapor mixture were measured relative to a known energy-pooling rate coefficient of a homonuclear reaction [i. e., Cs(6P) + Cs(5D) --> Cs(6S) + Cs (7D(J))]. Populations of the Cs(6P, 5D) and K(4P) states were produced by photodissociation of K2 and Cs2 molecules through the use of a dye laser radiation. The resulting fluorescence included the direct components emitted in the decay of the excited states produced by photodissociation and the induced components arising from the collisionally populated states. By combining relative intensities of the components with the effective lifetimes of Cs(6P) and K(4P) states, the rate coefficients (in units of 10(-9) cm3 x s(-1)) for the heteronuclear energy-pooling were found to be 2.6 and 3.6, respectively. The contribution to the rate coefficients from other processes are discussed.

21 CFR 880.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 880.9 Section 880.9 Food and Drugs FOOD AND DRUG... Food, Drug, and Cosmetic Act (the act). The exemption from the requirement of premarket notification...

21 CFR 878.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 878.9 Section 878.9 Food and Drugs FOOD AND DRUG..., and Cosmetic Act (the act). The exemption from the requirement of premarket notification (section 510...

21 CFR 884.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 884.9 Section 884.9 Food and Drugs FOOD AND DRUG..., and Cosmetic Act (the act). The exemption from the requirement of premarket notification (section 510...

21 CFR 876.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 876.9 Section 876.9 Food and Drugs FOOD AND DRUG..., and Cosmetic Act (the act). The exemption from the requirement of premarket notification (section 510...

21 CFR 862.9 - Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Limitations of exemptions from section 510(k) of the Federal Food, Drug, and Cosmetic Act (the act). 862.9 Section 862.9 Food and Drugs FOOD AND DRUG... Federal Food, Drug, and Cosmetic Act (the act). The exemption from the requirement of premarket...

33 CFR 55.9 - Child development centers.

Science.gov (United States)

2010-07-01

.... (c) Training programs shall be conducted monthly to ensure that all child development center employees complete a minimum of 20 hours of training annually with respect to early childhood development... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Child development centers. 55.9...

Suspension culture process for H9N2 avian influenza virus (strain Re-2).

Science.gov (United States)

Wang, Honglin; Guo, Suying; Li, Zhenguang; Xu, Xiaoqin; Shao, Zexiang; Song, Guicai

2017-10-01

H9N2 avian influenza virus has caused huge economic loss for the Chinese poultry industry since it was first identified. Vaccination is frequently used as a control method for the disease. Meanwhile suspension culture has become an important tool for the development of influenza vaccines. To optimize the suspension culture conditions for the avian influenza H9N2 virus (Re-2 strain) in Madin-Darby Canine Kidney (MDCK) cells, we studied the culture conditions for cell growth and proliferation parameters for H9N2 virus replication. MDCK cells were successfully cultured in suspension, from a small scale to industrial levels of production, with passage time and initial cell density being optimized. The influence of pH on the culture process in the reactor has been discussed and the process parameters for industrial production were explored via amplification of the 650L reactor. Subsequently, we cultivated cells at high cell density and harvested high amounts of virus, reaching 10log2 (1:1024). Furthermore an animal experiment was conducted to detect antibody. Compared to the chicken embryo virus vaccine, virus cultured from MDCK suspension cells can produce a higher amount of antibodies. The suspension culture process is simple and cost efficient, thus providing a solid foundation for the realization of large-scale avian influenza vaccine production.

Development of Preliminary HT9 Cladding Tube for Sodium-cooled Fast Reactor (SFR)

International Nuclear Information System (INIS)

Kim, Jun Hwan; Baek, Jong Hyuk; Heo, Hyeong Min; Park, Sang Gyu; Kim, Sung Ho; Lee, Chan Bock

2013-01-01

To achieve manufacturing technology of the fuel cladding tube in order to keep pace with the predetermined schedule in developing SFR fuel, KAERI has launched in developing fuel cladding tube in cooperation with a domestic steelmaking company. After fabricating medium-sized 1.1 ton HT9 ingot, followed by the multiple processes of hot and cold working, preliminary samples of HT9 seamless cladding tube having 7.4mm in outer diameter, 0.56mm in thickness, and 3m in length were fabricated. The objective of this study is to summarize the brief development status of the HT9 cladding tubes. Mechanical properties like axial tension, biaxial burst, pressurized creep and sodium compatibility of the cladding tubes were carried out to set up the performance evaluation technology to test the prototype FMS cladding tube which is going to be manufactured in next stage. As a part of developing fuel cladding for the Sodium-cooled Fast Reactor (SFR), preliminary HT9 cladding tube was fabricated in cooperation with a domestic steelmaking company. Microstructure as well as mechanical tests like axial tensile test, biaxial burst test, and pressurized creep test of the fuel cladding were carried out. Performance of the domestic HT9 tube was revealed to be similar in the previously fabricated foreign HT9 tube. Further prototype FMS cladding tube is going to be manufactured in next year based on this experience. Various test items like mechanical test, sodium compatibility test, microstructural analysis, basic property, cladding performance under transient situation, and performance under ion and neutron irradiation are going be performed in the future to set up the relevant technology for the licensing of the SFR cladding tube

IL-15 up-regulates the MMP-9 expression levels and induces inflammatory infiltration of macrophages in polymyositis through regulating the NF-kB pathway.

Science.gov (United States)

Yan, Wang; Fan, Weinv; Chen, Caijing; Wu, Yunqin; Fan, Zhenyi; Chen, Jiaqi; Chen, Zhaoying; Chen, Huimin

2016-10-10

This study was aimed to research the effects of IL-15 on inducing inflammatory infiltration of macrophages in polymyositis (PM) through the NF-kB pathway, and whether IL-15 was able to further regulate MMP-9 expression levels. Prepared PM cells, collected from the patients suffering from PM, were administered to SD rats. Also, a group of healthy SD rats was undergoing the same treatment as the control group. The test animals were treated with either anti-IL-15, IL-15, MMP-9 siRNA or ERK1/2 inhibitor. The blood toxicological parameters creatine kinase (CK) and CD163 were tested by using ELISA and immunohistochemistry assay. In addition, NF-kB expression in macrophages was measured by immunocytochemical assay. To measure the degree of cell infiltration the Transwell assay was performed. Lastly, western blot and zymography were carried out to compare MMP-9 and ERK expression levels between the two groups, both in vivo and in vitro. The results showed that S-CK, IL-15 and IL-15Rα levels increased rapidly after the conventional treatment was introduced to the PM infected SD rats. The PM model establishment and IL-15 treatment significantly increased the expressions of IL-15Rα, MMP-9, p-ERK and p-IKBα. However, the same effect can be suppressed by using anti-IL-15, MMP-9 siRNA or ERK1/2 inhibitor (P kB in the macrophages. IL-15 is able to significantly regulate the inflammatory infiltration of macrophages in PM patients through affecting the NF-kB pathway and MMP-9 expression levels. Copyright © 2016 Elsevier B.V. All rights reserved.

Early postnatal feed restriction reduces liver connective tissue levels and affects H3K9 acetylation state of regulated genes associated with protein metabolism in low birth weight pigs.

Science.gov (United States)

Nebendahl, Constance; Görs, Solvig; Albrecht, Elke; Krüger, Ricarda; Martens, Karen; Giller, Katrin; Hammon, Harald M; Rimbach, Gerald; Metges, Cornelia C

2016-03-01

Intrauterine growth retardation is associated with metabolic consequences in adulthood. Since our previous data indicate birth weight-dependent effects of feed restriction (R) on protein degradation processes in the liver, it should be investigated whether effects on connective tissue turnover are obvious and could be explained by global changes of histone H3K9me3 and H3K9ac states in regulated genes. For this purpose, female littermate pigs with low (U) or normal (N) birth weight were subjected to 3-week R (60% of ad libitum fed controls) with subsequent refeeding (REF) for further 5 weeks. The 3-week R-period induced a significant reduction of connective tissue area by 43% in the liver of U animals at 98 d of age, which was not found in age-matched N animals. Of note, after REF at 131 d of age, in previously feed-restricted U animals (UR), the percentage of mean connective tissue was only 53% of ad libitum fed controls (UK), indicating a persistent effect. In U animals, R induced H3K9 acetylation of regulated genes (e.g. XBP1, ERLEC1, GALNT2, PTRH2), which were inter alia associated with protein metabolism. In contrast, REF was mostly accompanied by deacetylation in U and N animals. Thus, our epigenetic data may give a first explanation for the observed birth weight-dependent differences in this connective tissue phenotype. Copyright © 2015 Elsevier Inc. All rights reserved.

Operating software for plutonium measurements in the KfK K-edge densitometer. A user's guide

International Nuclear Information System (INIS)

Eberle, H.

1983-09-01

A set of computer programs has been developed for the execution of plutonium concentration measurements in the KfK K-edge photon absorptiometer for performance evaluation. The programs are implemented on a ND-6620 data acquisition and processing system. A short description of the programs and commands for data accumulation and analysis is given. Nevertheless, the user should be familiar with the ND-6620 data acquisition and processing system, its system software, and with the FORTRAN programming language. (orig./HP)

Development of safe and shelf-stable Intermediate Moisture (IM) convenience meat products through radiation processing

International Nuclear Information System (INIS)

Ramesh Chander; Kanatt, S.R.; Chawla, S.P.; Bongirwar, D.R.

2001-01-01

Ready-to-use shelf stable mutton and chicken sheek kababs, and chicken chilly were developed by reducing the water activity either by grilling or by hot air drying, vacuum packing and irradiation. Microbiological analysis revealed a dose dependent reduction in total viable count and in Staphylococcus species on irradiation treatment ( 2.5, 5.0 and 10.0 kGy). The products subjected to irradiation at 10 kGy showed absence of viable micro-organisms and also had high sensory acceptability up to 9 months at ambient temperature. (author)

DZNep, inhibitor of S-adenosylhomocysteine hydrolase, down-regulates expression of SETDB1 H3K9me3 HMTase in human lung cancer cells.

Science.gov (United States)

Lee, Ju-Kyung; Kim, Keun-Cheol

2013-09-06

3-Deazaneplanocin A (DZNep), an epigenetic anticancer drug, leads to the indirect suppression of S-adenosyl methionine-dependent cellular methylations by inhibiting S-adenosyl homocystein (AdoHcy) hydrolase. Although it is well known that DZNep targets the degradation of EZH2 protein, H3K27me3 HMTase, there are still uncertainties about the regulation of other types of HMTases during cell death. In this study, we describe that SETDB1 gene expression was regulated by DZNep treatment in human lung cancer cells. We confirm that DZNep induced growth inhibition and increased the dead cell population of lung cancer cells. DZNep treatment affected histone methylations, including H3K27me3 and H3K9me3, but not H3K4me3. Reduced levels of H3K27me3 and H3K9me3 were related with the decreased EZH2 and SETDB1 proteins. Real time PCR analysis showed that SETDB1 gene expression was decreased by DZNep treatment, but no effect was observed for EZH2 gene expression. We cloned the promoter region of SETDB1 and SUV39H1 genes, and performed luciferase assays. The promoter activity of SETDB1 gene was down regulated by DZNep treatment, whereas no effect on SUV39H1 promoter activity was observed. In conclusion, we suggest that DZNep regulates not only on H3K27me3 HMTase EZH2, but also H3K9 HMTase SETDB1 gene expression at the transcription level, implicating that the mechanism of action of DZNep targets multiple HMTases during the death of lung cancer cells. Copyright © 2013 Elsevier Inc. All rights reserved.

Development of a space-flight ADR providing continuous cooling at 50 mK with heat rejection at 10 K

Science.gov (United States)

Tuttle, James; Canavan, Edgar; DeLee, Hudson; DiPirro, Michael; Jahromi, Amir; James, Bryan; Kimball, Mark; Shirron, Peter; Sullivan, Dan; Switzer, Eric

2017-12-01

Future astronomical instruments will require sub-Kelvin detector temperatures to obtain high sensitivity. In many cases large arrays of detectors will be used, and the associated cooling systems will need performance surpassing the limits of present technologies. NASA is developing a compact cooling system that will lift heat continuously at temperatures below 50 mK and reject it at over 10 K. Based on adiabatic demagnetization refrigerators (ADRs), it will have high thermodynamic efficiency and vibration-free operation with no moving parts. It will provide more than 10 times the current flight ADR cooling power at 50 mK and will also continuously cool a 4 K stage for instruments and optics. In addition, it will include an advanced magnetic shield resulting in external field variations below 5 μT. We describe the cooling system here and report on the progress in its development.

1000kW on-site PAFC power plant development and demonstration

Energy Technology Data Exchange (ETDEWEB)

Satomi, Tomohide; Koike, Shunichi [Phosphoric Acid Fuel Cell Technology Research Association (PAFC-TRA), Osaka (Japan); Ishikawa, Ryou [New Energy and Industrial Technology Development Organization (NEDO), Tokyo (Japan)

1996-12-31

Phosphoric Acid Fuel Cell Technology Research Association (PAFC-TRA) and New Energy and Industrial Technology Development Organization (NEDO) have been conducting a joint project on development of a 5000kW urban energy center type PAFC power plant (pressurized) and a 1000kW on-site PAFC power plant (non-pressurized). The objective of the technical development of 1000kW on-site PAFC power plant is to realize a medium size power plant with an overall efficiency of over 70% and an electrical efficiency of over 36%, that could be installed in a large building as a cogeneration system. The components and system integration development work and the plant design were performed in 1991 and 1992. Manufacturing of the plant and installation at the test site were completed in 1994. PAC test was carried out in 1994, and generation test was started in January 1995. Demonstration test is scheduled for 1995 and 1996.

The typical steps of radiation processes development. Experience in investigation, designing and application

International Nuclear Information System (INIS)

Babkin, I. Yu.

1983-01-01

The typical steps of radiation processes development are discussed as: primary laboratory investigations; primary economic evaluation; more exact estimation of situation in industry; comparative analysis; development of a flow sheet; pilot plant; obtaining of initial data for designing of industrial scale plant; prediction of industrial situation; designing of semi-industrial or industrial plant; industrial plant. (U.K.)

Design, development and evaluation of rK28-based point-of-care tests for improving rapid diagnosis of visceral leishmaniasis.

Directory of Open Access Journals (Sweden)

Sowmya Pattabhi

Full Text Available BACKGROUND: Visceral leishmaniasis (VL is diagnosed by microscopic confirmation of the parasite in bone marrow, spleen or lymph node aspirates. These procedures are unsuitable for rapid diagnosis of VL in field settings. The development of rK39-based rapid diagnostic tests (RDT revolutionized diagnosis of VL by offering high sensitivity and specificity in detecting disease in the Indian subcontinent; however, these tests have been less reliable in the African subcontinent (sensitivity range of 75-85%, specificity of 70-92%. We have addressed limitations of the rK39 with a new synthetic polyprotein, rK28, followed by development and evaluation of two new rK28-based RDT prototype platforms. METHODOLOGY/PRINCIPAL FINDINGS: Evaluation of 62 VL-confirmed sera from Sudan provided sensitivities of 96.8% and 93.6% (95% CI = K28: 88.83-99.61%; K39: 84.30-98.21% and specificities of 96.2% and 92.4% (95% CI = K28: 90.53-98.95%; K39: 85.54-96.65% for rK28 and rK39, respectively. Of greater interest was the observation that individual VL sera with low rK39 reactivity often had much higher rK28 reactivity. This characteristic of the fusion protein was exploited in the development of rK28 rapid tests, which may prove to be crucial in detecting VL among patients with low rK39 antibody levels. Evaluation of two prototype lateral flow-based rK28 rapid tests on 53 VL patients in Sudan and 73 VL patients in Bangladesh provided promisingly high sensitivities (95.9% [95% CI = 88.46-99.1 in Sudan and 98.1% [95% CI = 89.93-99.95%] in Bangladesh compared to the rK39 RDT (sensitivities of 86.3% [95% CI = 76.25-93.23%] in Sudan and 88.7% [95% CI = 76.97-95.73%] in Bangladesh. CONCLUSIONS/SIGNIFICANCE: Our study compares the diagnostic accuracy of rK39 and rK28 in detecting active VL cases and our findings indicate that rK28 polyprotein has great potential as a serodiagnostic tool. A new rK28-based RDT will prove to be a valuable asset in

Inhibition of H3K9me2 Reduces Hair Cell Regeneration after Hair Cell Loss in the Zebrafish Lateral Line by Down-Regulating the Wnt and Fgf Signaling Pathways

Science.gov (United States)

Tang, Dongmei; Lin, Qin; He, Yingzi; Chai, Renjie; Li, Huawei

2016-01-01

The activation of neuromast (NM) supporting cell (SC) proliferation leads to hair cell (HC) regeneration in the zebrafish lateral line. Epigenetic mechanisms have been reported that regulate HC regeneration in the zebrafish lateral line, but the role of H3K9me2 in HC regeneration after HC loss remains poorly understood. In this study, we focused on the role of H3K9me2 in HC regeneration following neomycin-induced HC loss. To investigate the effects of H3K9me2 in HC regeneration, we took advantage of the G9a/GLP-specific inhibitor BIX01294 that significantly reduces the dimethylation of H3K9. We found that BIX01294 significantly reduced HC regeneration after neomycin-induced HC loss in the zebrafish lateral line. BIX01294 also significantly reduced the proliferation of NM cells and led to fewer SCs in the lateral line. In situ hybridization showed that BIX01294 significantly down-regulated the Wnt and Fgf signaling pathways, which resulted in reduced SC proliferation and HC regeneration in the NMs of the lateral line. Altogether, our results suggest that down-regulation of H3K9me2 significantly decreases HC regeneration after neomycin-induced HC loss through inactivation of the Wnt/β-catenin and Fgf signaling pathways. Thus H3K9me2 plays a critical role in HC regeneration. PMID:27303264

Inhibition of H3K9me2 Reduces Hair Cell Regeneration after Hair Cell Loss in the Zebrafish Lateral Line by Down-Regulating the Wnt and Fgf Signaling Pathways

Directory of Open Access Journals (Sweden)

Dongmei eTang

2016-05-01

Full Text Available The activation of neuromast supporting cell (SC proliferation leads to hair cell (HC regeneration in the zebrafish lateral line. Epigenetic mechanisms have been reported that regulate HC regeneration in the zebrafish lateral line, but the role of H3K9me2 in HC regeneration after HC loss remains poorly understood. In this study, we focused on the role of H3K9me2 in HC regeneration following neomycin-induced HC loss. To investigate the effects of H3K9me2 in HC regeneration, we took advantage of the G9a/GLP-specific inhibitor BIX01294 that significantly reduces the dimethylation of H3K9. We found that BIX01294 significantly reduced HC regeneration after neomycin-induced HC loss in the zebrafish lateral line. BIX01294 also significantly reduced the proliferation of neuromast cells and led to fewer SCs in the lateral line. In situ hybridization showed that BIX01294 significantly down-regulated the Wnt and Fgf signaling pathways, which resulted in reduced SC proliferation and HC regeneration in the neuromasts of the lateral line. Altogether, our results suggest that down-regulation of H3K9me2 significantly decreases HC regeneration after neomycin-induced HC loss through inactivation of the Wnt/β-catenin and Fgf signaling pathways. Thus H3K9me2 plays a critical role in HC regeneration.

Proteinase K processing of rabbit muscle creatine kinase

DEFF Research Database (Denmark)

Leydier, C; Andersen, Jens S.; Couthon, F

1997-01-01

Proteinase K cleaves selectively both cytosolic and mitochondrial isoforms of creatine kinase leading to the appearance of two fragments, a large N-terminal one (K1) and a small C-terminal peptide (K2) which remain associated together. The loss of enzymatic activity correlates with the extent...... of monomer cleavage. N-terminal sequencing of the K2 fragments from rabbit cytosolic and pig mitochondrial creatine kinase shows that these peptides begin with A328 and A324, respectively. Electrospray ionization mass spectrometry demonstrates that K2 peptide is composed of 53 residues (A328-K380). However...

A de novo 1.58 Mb deletion, including MAP2K6 and mapping 1.28 Mb upstream to SOX9, identified in a patient with Pierre Robin sequence and osteopenia with multiple fractures.

Science.gov (United States)

Smyk, Marta; Roeder, Elizabeth; Cheung, Sau Wai; Szafranski, Przemyslaw; Stankiewicz, Paweł

2015-08-01

Defects of long-range regulatory elements of dosage-sensitive genes represent an under-recognized mechanism underlying genetic diseases. Haploinsufficiency of SOX9, the gene essential for development of testes and differentiation of chondrocytes, results in campomelic dysplasia, a skeletal malformation syndrome often associated with sex reversal. Chromosomal rearrangements with breakpoints mapping up to 1.6 Mb up- and downstream to SOX9, and disrupting its distant cis-regulatory elements, have been described in patients with milder forms of campomelic dysplasia, Pierre Robin sequence, and sex reversal. We present an ∼1.58 Mb deletion mapping ∼1.28 Mb upstream to SOX9 that encompasses its putative long-range cis-regulatory element(s) and MAP2K6 in a patient with Pierre Robin sequence and osteopenia with multiple fractures. Low bone mass panel testing using massively parallel sequencing of 23 nuclear genes, including COL1A1 and COL1A2 was negative. Based on the previous mouse model of Map2k6, suggesting that Sox9 is likely a downstream target of the p38 MAPK pathway, and our previous chromosome conformation capture-on-chip (4C) data showing potential interactions between SOX9 promoter and MAP2K6, we hypothesize that deletion of MAP2K6 might have affected SOX9 expression and contributed to our patient's phenotype. © 2015 Wiley Periodicals, Inc.

Development of heart failure is independent of K+ channel-interacting protein 2 expression

Science.gov (United States)

Speerschneider, Tobias; Grubb, Søren; Metoska, Artina; Olesen, Søren-Peter; Calloe, Kirstine; Thomsen, Morten B

2013-01-01

Abnormal ventricular repolarization in ion channelopathies and heart disease is a major cause of ventricular arrhythmias and sudden cardiac death. K+ channel-interacting protein 2 (KChIP2) expression is significantly reduced in human heart failure (HF), contributing to a loss of the transient outward K+ current (Ito). We aim to investigate the possible significance of a changed KChIP2 expression on the development of HF and proarrhythmia. Transverse aortic constrictions (TAC) and sham operations were performed in wild-type (WT) and KChIP2−/− mice. Echocardiography was performed before and every 2 weeks after the operation. Ten weeks post-surgery, surface ECG was recorded and we paced the heart in vivo to induce arrhythmias. Afterwards, tissue from the left ventricle was used for immunoblotting. Time courses of HF development were comparable in TAC-operated WT and KChIP2−/− mice. Ventricular protein expression of KChIP2 was reduced by 70% after 10 weeks TAC in WT mice. The amplitudes of the J and T waves were enlarged in KChIP2−/− control mice. Ventricular effective refractory period, RR, QRS and QT intervals were longer in mice with HF compared to sham-operated mice of either genotype. Pacing-induced ventricular tachycardia (VT) was observed in 5/10 sham-operated WT mice compared with 2/10 HF WT mice with HF. Interestingly, and contrary to previously published data, sham-operated KChIP2−/− mice were resistant to pacing-induced VT resulting in only 1/10 inducible mice. KChIP2−/− with HF mice had similar low vulnerability to inducible VT (1/9). Our results suggest that although KChIP2 is downregulated in HF, it is not orchestrating the development of HF. Moreover, KChIP2 affects ventricular repolarization and lowers arrhythmia susceptibility. Hence, downregulation of KChIP2 expression in HF may be antiarrhythmic in mice via reduction of the fast transient outward K+ current. PMID:24099801

Characterization of process-induced damage in Cu/low-k interconnect structure by microscopic infrared spectroscopy with polarized infrared light

Energy Technology Data Exchange (ETDEWEB)

Seki, Hirofumi, E-mail: Hirofumi-Seki@trc.toray.co.jp; Hashimoto, Hideki [Toray Research Center, Inc., 3-3-7 Sonoyama, Otsu, Shiga 520-8567 (Japan); Ozaki, Yukihiro [Department of Chemistry, School of Science and Technology, Kwansei Gakuin University, 2-1, Gakuen, Sanda, Hyogo 669-1337 (Japan)

2016-09-07

Microscopic Fourier-transform infrared (FT-IR) spectra are measured for a Cu/low-k interconnect structure using polarized IR light for different widths of low-k spaces and Cu lines, and for different heights of Cu lines, on Si substrates. Although the widths of the Cu line and the low-k space are 70 nm each, considerably smaller than the wavelength of the IR light, the FT-IR spectra of the low-k film were obtained for the Cu/low-k interconnect structure. A suitable method was established for measuring the process-induced damage in a low-k film that was not detected by the TEM-EELS (Transmission Electron Microscope-Electron Energy-Loss Spectroscopy) using microscopic IR polarized light. Based on the IR results, it was presumed that the FT-IR spectra mainly reflect the structural changes in the sidewalls of the low-k films for Cu/low-k interconnect structures, and the mechanism of generating process-induced damage involves the generation of Si-OH groups in the low-k film when the Si-CH{sub 3} bonds break during the fabrication processes. The Si-OH groups attract moisture and the OH peak intensity increases. It was concluded that the increase in the OH groups in the low-k film is a sensitive indicator of low-k damage. We achieved the characterization of the process-induced damage that was not detected by the TEM-EELS and speculated that the proposed method is applicable to interconnects with line and space widths of 70 nm/70 nm and on shorter scales of leading edge devices. The location of process-induced damage and its mechanism for the Cu/low-k interconnect structure were revealed via the measurement method.

Measurement of the CKM angle $\\gamma$ using $B^\\pm\\to DK^\\pm$ with $D\\to K_\\text{S}^0\\pi^+\\pi^-$, $K_\\text{S}^0K^+K^-$ decays

CERN Document Server

Aaij, Roel; LHCb Collaboration; Adinolfi, Marco; Aidala, Christine Angela; Ajaltouni, Ziad; Akar, Simon; Albicocco, Pietro; Albrecht, Johannes; Alessio, Federico; Alexander, Michael; Alfonso Albero, Alejandro; Ali, Suvayu; Alkhazov, Georgy; Alvarez Cartelle, Paula; Alves Jr, Antonio Augusto; Amato, Sandra; Amerio, Silvia; Amhis, Yasmine; An, Liupan; Anderlini, Lucio; Andreassi, Guido; Andreotti, Mirco; Andrews, Jason; Appleby, Robert; Archilli, Flavio; d'Argent, Philippe; Arnau Romeu, Joan; Artamonov, Alexander; Artuso, Marina; Arzymatov, Kenenbek; Aslanides, Elie; Atzeni, Michele; Bachmann, Sebastian; Back, John; Baker, Sophie; Balagura, Vladislav; Baldini, Wander; Baranov, Alexander; Barlow, Roger; Barsuk, Sergey; Barter, William; Baryshnikov, Fedor; Batozskaya, Varvara; Batsukh, Baasansuren; Battista, Vincenzo; Bay, Aurelio; Beddow, John; Bedeschi, Franco; Bediaga, Ignacio; Beiter, Andrew; Bel, Lennaert; Beliy, Nikita; Bellee, Violaine; Belloli, Nicoletta; Belous, Konstantin; Belyaev, Ivan; Ben-Haim, Eli; Bencivenni, Giovanni; Benson, Sean; Beranek, Sarah; Berezhnoy, Alexander; Bernet, Roland; Berninghoff, Daniel; Bertholet, Emilie; Bertolin, Alessandro; Betancourt, Christopher; Betti, Federico; Bettler, Marc-Olivier; van Beuzekom, Martinus; Bezshyiko, Iaroslava; Bhasin, Srishti; Bhom, Jihyun; Bian, Lingzhu; Bifani, Simone; Billoir, Pierre; Birnkraut, Alex; Bizzeti, Andrea; Bjørn, Mikkel; Blago, Michele Piero; Blake, Thomas; Blanc, Frederic; Blusk, Steven; Bobulska, Dana; Bocci, Valerio; Boente Garcia, Oscar; Boettcher, Thomas; Bondar, Alexander; Bondar, Nikolay; Borghi, Silvia; Borisyak, Maxim; Borsato, Martino; Bossu, Francesco; Boubdir, Meriem; Bowcock, Themistocles; Bozzi, Concezio; Braun, Svende; Brodski, Michael; Brodzicka, Jolanta; Brundu, Davide; Buchanan, Emma; Buonaura, Annarita; Burr, Christopher; Bursche, Albert; Buytaert, Jan; Byczynski, Wiktor; Cadeddu, Sandro; Cai, Hao; Calabrese, Roberto; Calladine, Ryan; Calvi, Marta; Calvo Gomez, Miriam; Camboni, Alessandro; Campana, Pierluigi; Campora Perez, Daniel Hugo; Capriotti, Lorenzo; Carbone, Angelo; Carboni, Giovanni; Cardinale, Roberta; Cardini, Alessandro; Carniti, Paolo; Carson, Laurence; Carvalho Akiba, Kazuyoshi; Casse, Gianluigi; Cassina, Lorenzo; Cattaneo, Marco; Cavallero, Giovanni; Cenci, Riccardo; Chamont, David; Chapman, Matthew George; Charles, Matthew; Charpentier, Philippe; Chatzikonstantinidis, Georgios; Chefdeville, Maximilien; Chekalina, Viktoriia; Chen, Chen; Chen, Shanzhen; Chitic, Stefan-Gabriel; Chobanova, Veronika; Chrzaszcz, Marcin; Chubykin, Alexsei; Ciambrone, Paolo; Cid Vidal, Xabier; Ciezarek, Gregory; Clarke, Peter; Clemencic, Marco; Cliff, Harry; Closier, Joel; Coco, Victor; Cogan, Julien; Cogneras, Eric; Cojocariu, Lucian; Collins, Paula; Colombo, Tommaso; Comerma-Montells, Albert; Contu, Andrea; Coombs, George; Coquereau, Samuel; Corti, Gloria; Corvo, Marco; Costa Sobral, Cayo Mar; Couturier, Benjamin; Cowan, Greig; Craik, Daniel Charles; Crocombe, Andrew; Cruz Torres, Melissa Maria; Currie, Robert; D'Ambrosio, Carmelo; Da Cunha Marinho, Franciole; Da Silva, Cesar Luiz; Dall'Occo, Elena; Dalseno, Jeremy; Danilina, Anna; Davis, Adam; De Aguiar Francisco, Oscar; De Bruyn, Kristof; De Capua, Stefano; De Cian, Michel; De Miranda, Jussara; De Paula, Leandro; De Serio, Marilisa; De Simone, Patrizia; Dean, Cameron Thomas; Decamp, Daniel; Del Buono, Luigi; Delaney, Blaise; Dembinski, Hans Peter; Demmer, Moritz; Dendek, Adam; Derkach, Denis; Deschamps, Olivier; Desse, Fabrice; Dettori, Francesco; Dey, Biplab; Di Canto, Angelo; Di Nezza, Pasquale; Didenko, Sergey; Dijkstra, Hans; Dordei, Francesca; Dorigo, Mirco; Dosil Suárez, Alvaro; Douglas, Lauren; Dovbnya, Anatoliy; Dreimanis, Karlis; Dufour, Laurent; Dujany, Giulio; Durante, Paolo; Durham, John Matthew; Dutta, Deepanwita; Dzhelyadin, Rustem; Dziewiecki, Michal; Dziurda, Agnieszka; Dzyuba, Alexey; Easo, Sajan; Egede, Ulrik; Egorychev, Victor; Eidelman, Semen; Eisenhardt, Stephan; Eitschberger, Ulrich; Ekelhof, Robert; Eklund, Lars; Ely, Scott; Ene, Alexandru; Escher, Stephan; Esen, Sevda; Evans, Timothy; Falabella, Antonio; Farley, Nathanael; Farry, Stephen; Fazzini, Davide; Federici, Luca; Fernandez, Gerard; Fernandez Declara, Placido; Fernandez Prieto, Antonio; Ferrari, Fabio; Ferreira Lopes, Lino; Ferreira Rodrigues, Fernando; Ferro-Luzzi, Massimiliano; Filippov, Sergey; Fini, Rosa Anna; Fiorini, Massimiliano; Firlej, Miroslaw; Fitzpatrick, Conor; Fiutowski, Tomasz; Fleuret, Frederic; Fontana, Marianna; Fontanelli, Flavio; Forty, Roger; Franco Lima, Vinicius; Frank, Markus; Frei, Christoph; Fu, Jinlin; Funk, Wolfgang; Färber, Christian; Féo Pereira Rivello Carvalho, Mauricio; Gabriel, Emmy; Gallas Torreira, Abraham; Galli, Domenico; Gallorini, Stefano; Gambetta, Silvia; Gandelman, Miriam; Gandini, Paolo; Gao, Yuanning; Garcia Martin, Luis Miguel; Garcia Plana, Beatriz; García Pardiñas, Julián; Garra Tico, Jordi; Garrido, Lluis; Gascon, David; Gaspar, Clara; Gavardi, Laura; Gazzoni, Giulio; Gerick, David; Gersabeck, Evelina; Gersabeck, Marco; Gershon, Timothy; Gerstel, Dawid; Ghez, Philippe; Gianì, Sebastiana; Gibson, Valerie; Girard, Olivier Göran; Giubega, Lavinia-Helena; Gizdov, Konstantin; Gligorov, Vladimir; Golubkov, Dmitry; Golutvin, Andrey; Gomes, Alvaro; Gorelov, Igor Vladimirovich; Gotti, Claudio; Govorkova, Ekaterina; Grabowski, Jascha Peter; Graciani Diaz, Ricardo; Granado Cardoso, Luis Alberto; Graugés, Eugeni; Graverini, Elena; Graziani, Giacomo; Grecu, Alexandru; Greim, Roman; Griffith, Peter; Grillo, Lucia; Gruber, Lukas; Gruberg Cazon, Barak Raimond; Grünberg, Oliver; Gu, Chenxi; Gushchin, Evgeny; Guz, Yury; Gys, Thierry; Göbel, Carla; Hadavizadeh, Thomas; Hadjivasiliou, Christos; Haefeli, Guido; Haen, Christophe; Haines, Susan; Hamilton, Brian; Han, Xiaoxue; Hancock, Thomas Henry; Hansmann-Menzemer, Stephanie; Harnew, Neville; Harnew, Samuel; Harrison, Thomas; Hasse, Christoph; Hatch, Mark; He, Jibo; Hecker, Malte; Heinicke, Kevin; Heister, Arno; Hennessy, Karol; Henry, Louis; van Herwijnen, Eric; Heß, Miriam; Hicheur, Adlène; Hill, Donal; Hilton, Martha; Hopchev, Plamen Hristov; Hu, Wenhua; Huang, Wenqian; Huard, Zachary; Hulsbergen, Wouter; Humair, Thibaud; Hushchyn, Mikhail; Hutchcroft, David; Ibis, Philipp; Idzik, Marek; Ilten, Philip; Ivshin, Kuzma; Jacobsson, Richard; Jalocha, Pawel; Jans, Eddy; Jawahery, Abolhassan; Jiang, Feng; John, Malcolm; Johnson, Daniel; Jones, Christopher; Joram, Christian; Jost, Beat; Jurik, Nathan; Kandybei, Sergii; Karacson, Matthias; Kariuki, James Mwangi; Karodia, Sarah; Kazeev, Nikita; Kecke, Matthieu; Keizer, Floris; Kelsey, Matthew; Kenzie, Matthew; Ketel, Tjeerd; Khairullin, Egor; Khanji, Basem; Khurewathanakul, Chitsanu; Kim, Kyung Eun; Kirn, Thomas; Klaver, Suzanne; Klimaszewski, Konrad; Klimkovich, Tatsiana; Koliiev, Serhii; Kolpin, Michael; Kopecna, Renata; Koppenburg, Patrick; Kostiuk, Igor; Kotriakhova, Sofia; Kozeiha, Mohamad; Kravchuk, Leonid; Kreps, Michal; Kress, Felix Johannes; Krokovny, Pavel; Krupa, Wojciech; Krzemien, Wojciech; Kucewicz, Wojciech; Kucharczyk, Marcin; Kudryavtsev, Vasily; Kuonen, Axel Kevin; Kvaratskheliya, Tengiz; Lacarrere, Daniel; Lafferty, George; Lai, Adriano; Lancierini, Davide; Lanfranchi, Gaia; Langenbruch, Christoph; Latham, Thomas; Lazzeroni, Cristina; Le Gac, Renaud; Leflat, Alexander; Lefrançois, Jacques; Lefèvre, Regis; Lemaitre, Florian; Leroy, Olivier; Lesiak, Tadeusz; Leverington, Blake; Li, Pei-Rong; Li, Tenglin; Li, Zhuoming; Liang, Xixin; Likhomanenko, Tatiana; Lindner, Rolf; Lionetto, Federica; Lisovskyi, Vitalii; Liu, Xuesong; Loh, David; Loi, Angelo; Longstaff, Iain; Lopes, Jose; Lovell, George Holger; Lucchesi, Donatella; Lucio Martinez, Miriam; Lupato, Anna; Luppi, Eleonora; Lupton, Oliver; Lusiani, Alberto; Lyu, Xiao-Rui; Machefert, Frederic; Maciuc, Florin; Macko, Vladimir; Mackowiak, Patrick; Maddrell-Mander, Samuel; Maev, Oleg; Maguire, Kevin; Maisuzenko, Dmitrii; Majewski, Maciej Witold; Malde, Sneha; Malecki, Bartosz; Malinin, Alexander; Maltsev, Timofei; Manca, Giulia; Mancinelli, Giampiero; Marangotto, Daniele; Maratas, Jan; Marchand, Jean François; Marconi, Umberto; Marin Benito, Carla; Marinangeli, Matthieu; Marino, Pietro; Marks, Jörg; Martellotti, Giuseppe; Martin, Morgan; Martinelli, Maurizio; Martinez Santos, Diego; Martinez Vidal, Fernando; Massafferri, André; Matev, Rosen; Mathad, Abhijit; Mathe, Zoltan; Matteuzzi, Clara; Mauri, Andrea; Maurice, Emilie; Maurin, Brice; Mazurov, Alexander; McCann, Michael; McNab, Andrew; McNulty, Ronan; Mead, James Vincent; Meadows, Brian; Meaux, Cedric; Meier, Frank; Meinert, Nis; Melnychuk, Dmytro; Merk, Marcel; Merli, Andrea; Michielin, Emanuele; Milanes, Diego Alejandro; Millard, Edward James; Minard, Marie-Noelle; Minzoni, Luca; Mitzel, Dominik Stefan; Mogini, Andrea; Molina Rodriguez, Josue; Mombächer, Titus; Monroy, Igancio Alberto; Monteil, Stephane; Morandin, Mauro; Morello, Gianfranco; Morello, Michael Joseph; Morgunova, Olga; Moron, Jakub; Morris, Adam Benjamin; Mountain, Raymond; Muheim, Franz; Mulder, Mick; Murphy, Colm Harold; Murray, Donal; Müller, Dominik; Müller, Janine; Müller, Katharina; Müller, Vanessa; Naik, Paras; Nakada, Tatsuya; Nandakumar, Raja; Nandi, Anita; Nanut, Tara; Nasteva, Irina; Needham, Matthew; Neri, Nicola; Neubert, Sebastian; Neufeld, Niko; Neuner, Max; Nguyen, Thi Dung; Nguyen-Mau, Chung; Nieswand, Simon; Niet, Ramon; Nikitin, Nikolay; Nogay, Alla; O'Hanlon, Daniel Patrick; Oblakowska-Mucha, Agnieszka; Obraztsov, Vladimir; Ogilvy, Stephen; Oldeman, Rudolf; Onderwater, Gerco; Ossowska, Anna; Otalora Goicochea, Juan Martin; Owen, Patrick; Oyanguren, Maria Aranzazu; Pais, Preema Rennee; Palano, Antimo; Palutan, Matteo; Panshin, Gennady; Papanestis, Antonios; Pappagallo, Marco; Pappalardo, Luciano; Parker, William; Parkes, Christopher; Passaleva, Giovanni; Pastore, Alessandra; Patel, Mitesh; Patrignani, Claudia; Pearce, Alex; Pellegrino, Antonio; Penso, Gianni; Pepe Altarelli, Monica; Perazzini, Stefano; Pereima, Dmitrii; Perret, Pascal; Pescatore, Luca; Petridis, Konstantinos; Petrolini, Alessandro; Petrov, Aleksandr; Petrucci, Stefano; Petruzzo, Marco; Pietrzyk, Boleslaw; Pietrzyk, Guillaume; Pikies, Malgorzata; Pili, Martina; Pinci, Davide; Pinzino, Jacopo; Pisani, Flavio; Piucci, Alessio; Placinta, Vlad-Mihai; Playfer, Stephen; Plews, Jonathan; Plo Casasus, Maximo; Polci, Francesco; Poli Lener, Marco; Poluektov, Anton; Polukhina, Natalia; Polyakov, Ivan; Polycarpo, Erica; Pomery, Gabriela Johanna; Ponce, Sebastien; Popov, Alexander; Popov, Dmitry; Poslavskii, Stanislav; Potterat, Cédric; Price, Eugenia; Prisciandaro, Jessica; Prouve, Claire; Pugatch, Valery; Puig Navarro, Albert; Pullen, Hannah Louise; Punzi, Giovanni; Qian, Wenbin; Qin, Jia-Jia; Quagliani, Renato; Quintana, Boris; Rachwal, Bartlomiej; Rademacker, Jonas; Rama, Matteo; Ramos Pernas, Miguel; Rangel, Murilo; Ratnikov, Fedor; Raven, Gerhard; Ravonel Salzgeber, Melody; Reboud, Meril; Redi, Federico; Reichert, Stefanie; dos Reis, Alberto; Reiss, Florian; Remon Alepuz, Clara; Ren, Zan; Renaudin, Victor; Ricciardi, Stefania; Richards, Sophie; Rinnert, Kurt; Robbe, Patrick; Robert, Arnaud; Rodrigues, Ana Barbara; Rodrigues, Eduardo; Rodriguez Lopez, Jairo Alexis; Roehrken, Markus; Rogozhnikov, Alexey; Roiser, Stefan; Rollings, Alexandra Paige; Romanovskiy, Vladimir; Romero Vidal, Antonio; Rotondo, Marcello; Rudolph, Matthew Scott; Ruf, Thomas; Ruiz Vidal, Joan; Saborido Silva, Juan Jose; Sagidova, Naylya; Saitta, Biagio; Salustino Guimaraes, Valdir; Sanchez Gras, Cristina; Sanchez Mayordomo, Carlos; Sanmartin Sedes, Brais; Santacesaria, Roberta; Santamarina Rios, Cibran; Santimaria, Marco; Santovetti, Emanuele; Sarpis, Gediminas; Sarti, Alessio; Satriano, Celestina; Satta, Alessia; Saur, Miroslav; Savrina, Darya; Schael, Stefan; Schellenberg, Margarete; Schiller, Manuel; Schindler, Heinrich; Schmelling, Michael; Schmelzer, Timon; Schmidt, Burkhard; Schneider, Olivier; Schopper, Andreas; Schreiner, HF; Schubiger, Maxime; Schune, Marie Helene; Schwemmer, Rainer; Sciascia, Barbara; Sciubba, Adalberto; Semennikov, Alexander; Sepulveda, Eduardo Enrique; Sergi, Antonino; Serra, Nicola; Serrano, Justine; Sestini, Lorenzo; Seyfert, Paul; Shapkin, Mikhail; Shcheglov, Yury; Shears, Tara; Shekhtman, Lev; Shevchenko, Vladimir; Shmanin, Evgenii; Siddi, Benedetto Gianluca; Silva Coutinho, Rafael; Silva de Oliveira, Luiz Gustavo; Simi, Gabriele; Simone, Saverio; Skidmore, Nicola; Skwarnicki, Tomasz; Smeaton, John Gordon; Smith, Eluned; Smith, Iwan Thomas; Smith, Mark; Soares, Marcelo; Soares Lavra, Lais; Sokoloff, Michael; Soler, Paul; Souza De Paula, Bruno; Spaan, Bernhard; Spradlin, Patrick; Stagni, Federico; Stahl, Marian; Stahl, Sascha; Stefko, Pavol; Stefkova, Slavomira; Steinkamp, Olaf; Stemmle, Simon; Stenyakin, Oleg; Stepanova, Margarita; Stevens, Holger; Stone, Sheldon; Storaci, Barbara; Stracka, Simone; Stramaglia, Maria Elena; Straticiuc, Mihai; Straumann, Ulrich; Strokov, Sergey; Sun, Jiayin; Sun, Liang; Swientek, Krzysztof; Syropoulos, Vasileios; Szumlak, Tomasz; Szymanski, Maciej Pawel; T'Jampens, Stephane; Tang, Zhipeng; Tayduganov, Andrey; Tekampe, Tobias; Tellarini, Giulia; Teubert, Frederic; Thomas, Eric; van Tilburg, Jeroen; Tilley, Matthew James; Tisserand, Vincent; Tolk, Siim; Tomassetti, Luca; Tonelli, Diego; Tou, Da Yu; Tourinho Jadallah Aoude, Rafael; Tournefier, Edwige; Traill, Murdo; Tran, Minh Tâm; Trisovic, Ana; Tsaregorodtsev, Andrei; Tully, Alison; Tuning, Niels; Ukleja, Artur; Usachov, Andrii; Ustyuzhanin, Andrey; Uwer, Ulrich; Vacca, Claudia; Vagner, Alexander; Vagnoni, Vincenzo; Valassi, Andrea; Valat, Sebastien; Valenti, Giovanni; Vazquez Gomez, Ricardo; Vazquez Regueiro, Pablo; Vecchi, Stefania; van Veghel, Maarten; Velthuis, Jaap; Veltri, Michele; Veneziano, Giovanni; Venkateswaran, Aravindhan; Verlage, Tobias Anton; Vernet, Maxime; Vesterinen, Mika; Viana Barbosa, Joao Vitor; Vieira, Daniel; Vieites Diaz, Maria; Viemann, Harald; Vilasis-Cardona, Xavier; Vitkovskiy, Arseniy; Vitti, Marcela; Volkov, Vladimir; Vollhardt, Achim; Voneki, Balazs; Vorobyev, Alexey; Vorobyev, Vitaly; de Vries, Jacco; Vázquez Sierra, Carlos; Waldi, Roland; Walsh, John; Wang, Jianchun; Wang, Mengzhen; Wang, Yilong; Wang, Zhenzi; Ward, David; Wark, Heather Mckenzie; Watson, Nigel; Websdale, David; Weiden, Andreas; Weisser, Constantin; Whitehead, Mark; Wicht, Jean; Wilkinson, Guy; Wilkinson, Michael; Williams, Ifan; Williams, Mark Richard James; Williams, Mike; Williams, Timothy; Wilson, Fergus; Wimberley, Jack; Winn, Michael Andreas; Wishahi, Julian; Wislicki, Wojciech; Witek, Mariusz; Wormser, Guy; Wotton, Stephen; Wyllie, Kenneth; Xiao, Dong; Xie, Yuehong; Xu, Ao; Xu, Menglin; Xu, Qingnian; Xu, Zehua; Xu, Zhirui; Yang, Zhenwei; Yang, Zishuo; Yao, Yuezhe; Yeomans, Lauren Emma; Yin, Hang; Yu, Jiesheng; Yuan, Xuhao; Yushchenko, Oleg; Zarebski, Kristian Alexander; Zavertyaev, Mikhail; Zhang, Dongliang; Zhang, Liming; Zhang, Wen Chao; Zhang, Yanxi; Zhelezov, Alexey; Zheng, Yangheng; Zhu, Xianglei; Zhukov, Valery; Zonneveld, Jennifer Brigitta; Zucchelli, Stefano

2018-01-01

A binned Dalitz plot analysis of $B^\\pm \\to D K^\\pm$ decays, with $D\\to K_\\text{S}^0\\pi^+\\pi^-$ and $D\\to K_\\text{S}^0K^+K^-$, is used to perform a measurement of the CP-violating observables $x_{\\pm}$ and $y_{\\pm}$, which are sensitive to the Cabibbo-Kobayashi-Maskawa angle $\\gamma$. The analysis is performed without assuming any $D$ decay model, through the use of information on the strong-phase variation over the Dalitz plot from the CLEO collaboration. Using a sample of proton-proton collision data collected with the LHCb experiment in 2015 and 2016, and corresponding to an integrated luminosity of 2.0$\\,\\text{fb}^{-1}$, the values of the CP violation parameters are found to be $x_- = ( 9.0 \\pm 1.7 \\pm 0.7 \\pm 0.4) \\times 10^{-2}$, $y_- = ( 2.1 \\pm 2.2 \\pm 0.5 \\pm 1.1) \\times 10^{-2}$, $x_+ = (- 7.7 \\pm 1.9 \\pm 0.7 \\pm 0.4) \\times 10^{-2}$, and $y_+ = (- 1.0 \\pm 1.9 \\pm 0.4 \\pm 0.9) \\times 10^{-2}$. The first uncertainty is statistical, the second is systematic, and the third is due to the uncertainty on ...

Digital development of products with NX9 for academical areas

Science.gov (United States)

Goanta, A. M.

2015-11-01

International competitiveness forced the manufacturing enterprises to look for new ways to accelerate the development of digital products through innovation, global alliances and strategic partnerships. In an environment of global research and development of distributed geographically, all members of the joint teams made up of companies and universities need to access updated and accurate information about products created by any of the type employed, student, teacher. Current design processes involve more complex products consisting of elements of design created by multiple teams, disciplines and suppliers using independent CAD systems. Even when using a 3D CAD mature technology, many companies fail to significantly reduce losses in the process, improve product quality or product type to ensure successful innovations to market arouse interest. These challenges require a radical rethinking of the business model, which belongs to the field of design, which must be based on digital development of products based on integrated files. Through this work, the author has proposed to provide both synthesis and transformations brought news of the integrated NX [1, 2, 3] from Siemens PLM Software 9, following a news results detailed documentary study, and personal results obtained by applying the same version, the digital and integrated development of a product type device test beams. Based on educational license received for NX 9 was made a detailed study of the innovations made by this release, and the application of some of them went to graphical modelling and getting all the documentation of a test device bearing beams. Also, were synthesized in terms of methodology, the steps to take to obtain graphical documentation. The results consist of: 3D models of all parts and assembly 3D model of the three-dimensional constraints of all component parts and not least respectively all drawings and assembly drawing. The most important consequence of the paper is the obtaining of

Manipulation and handling processes off-line programming and optimization with use of K-Roset

Science.gov (United States)

Gołda, G.; Kampa, A.

2017-08-01

Contemporary trends in development of efficient, flexible manufacturing systems require practical implementation of modern “Lean production” concepts for maximizing customer value through minimizing all wastes in manufacturing and logistics processes. Every FMS is built on the basis of automated and robotized production cells. Except flexible CNC machine tools and other equipments, the industrial robots are primary elements of the system. In the studies, authors look for wastes of time and cost in real tasks of robots, during manipulation processes. According to aspiration for optimization of handling and manipulation processes with use of the robots, the application of modern off-line programming methods and computer simulation, is the best solution and it is only way to minimize unnecessary movements and other instructions. The modelling process of robotized production cell and offline programming of Kawasaki robots in AS-Language will be described. The simulation of robotized workstation will be realized with use of virtual reality software K-Roset. Authors show the process of industrial robot’s programs improvement and optimization in terms of minimizing the number of useless manipulator movements and unnecessary instructions. This is realized in order to shorten the time of production cycles. This will also reduce costs of handling, manipulations and technological process.

Final environmental impact statement, Beaufort Sea oil and gas development/Northstar Project. Appendices B through K

International Nuclear Information System (INIS)

1999-02-01

BP Exploration (Alaska) Inc. (BPXA) submitted a permit application to the US. Army Engineer District, Alaska to initiate the review process for BPXA's plans to develop and produce oil and gas from the Northstar Unit. This report contains Appendices B--K of an Environmental Impact Statement which was undertaken to identify and evaluate the potential effects the proposed project may have on the environment

Are You Ready for Y2K?

Science.gov (United States)

Day, C. William

1999-01-01

Examines the planning process to identify equipment or systems within an educational facility that could be Y2K sensitive. Discusses developing a contingency plan to prevent operational shutdown. Concluding comments describe a simple Y2K equipment-testing procedure. (GR)

Development of 750 keV/20kW DC accelerator

International Nuclear Information System (INIS)

Bapna, S.C.; Banwari, R.; Venkateswaran, S.V.; Tripathi, Alok; Kasliwal, Apollo; Pramod, R.; Kumar, Pankaj

2001-01-01

This paper discusses development of a DC accelerator at CAT for industrial applications. This accelerator is housed in two floors; first floor having the accelerator and the ground floor is an irradiation cell. It will operate in the voltage range of 300kV to 750kV and will give maximum beam power of 20kW. The electron gun, acceleration column, focusing coil, high voltage multiplier stack, filament power supply and the control unit are housed in a 1.5 m diameter 3.2 m high pressure vessel which will be pressurized to 5.5 bar of SF 6 gas

Summary status of K Basins sludge characterization

International Nuclear Information System (INIS)

Baker, R.B.

1995-01-01

A number of activities are underway as part of the Spent Nuclear Fuels Project (SNFP) related to the processing and disposing of sludge in the 105-K Basins (K Basins). Efforts to rigorously define data requirements for these activities are being made using the Data Quality Objectives (DQO) process. Summaries of current sludge characterization data are required to both help support this DQO process and to allow continued progress with on-going engineering activities (e.g., evaluations of disposal alternatives). This document provides the status of K Basins sludge characterization data currently available to the Nuclear Fuel Evaluations group. This group is tasked by the SNFP to help develop and maintain the characterization baseline for the K Basins. The specific objectives of this document are to: (1) provide a current summary (and set of references) of sludge characterization data for use by SNFP initiatives, to avoid unnecessary duplication of effort and to support on-going initiatives; (2) submit these data to an open forum for review and comment, and identify additional sources of significant data that may be available; (3) provide a summary of current data to use as part of the basis to develop requirements for additional sludge characterization data through the DQO process; (4) provide an overview of the intended activities that will be used to develop and maintain the sludge characterization baseline

Lauric Acid Stimulates Mammary Gland Development of Pubertal Mice through Activation of GPR84 and PI3K/Akt Signaling Pathway.

Science.gov (United States)

Meng, Yingying; Zhang, Jing; Zhang, Fenglin; Ai, Wei; Zhu, Xiaotong; Shu, Gang; Wang, Lina; Gao, Ping; Xi, Qianyun; Zhang, Yongliang; Liang, Xingwei; Jiang, Qingyan; Wang, Songbo

2017-01-11

It has been demonstrated that dietary fat affects pubertal mammary gland development. However, the role of lauric acid (LA) in this process remains unclear. Thus, this study aimed to investigate the effects of LA on mammary gland development in pubertal mice and to explore the underlying mechanism. In vitro, 100 μM LA significantly promoted proliferation of mouse mammary epithelial cell line HC11 by regulating expression of proliferative markers (cyclin D1/3, p21, PCNA). Meanwhile, LA activated the G protein-coupled receptor 84 (GPR84) and PI3K/Akt signaling pathway. In agreement, dietary 1% LA enhanced mammary duct development, increased the expression of GPR84 and cyclin D1, and activated PI3K/Akt in mammary gland of pubertal mice. Furthermore, knockdown of GPR84 or inhibition of PI3K/Akt totally abolished the promotion of HC11 proliferation induced by LA. These results showed that LA stimulated mammary gland development of pubertal mice through activation of GPR84 and PI3K/Akt signaling pathway.

Observation of $B^0_s \\to K^{*\\pm}K^\\mp$ and evidence for $B^0_s \\to K^{*-}\\pi^+$ decays

CERN Document Server

Aaij, Roel; Affolder, Anthony; Ajaltouni, Ziad; Akar, Simon; Albrecht, Johannes; Alessio, Federico; Alexander, Michael; Ali, Suvayu; Alkhazov, Georgy; Alvarez Cartelle, Paula; Alves Jr, Antonio; Amato, Sandra; Amerio, Silvia; Amhis, Yasmine; An, Liupan; Anderlini, Lucio; Anderson, Jonathan; Andreassen, Rolf; Andreotti, Mirco; Andrews, Jason; Appleby, Robert; Aquines Gutierrez, Osvaldo; Archilli, Flavio; Artamonov, Alexander; Artuso, Marina; Aslanides, Elie; Auriemma, Giulio; Baalouch, Marouen; Bachmann, Sebastian; Back, John; Badalov, Alexey; Baldini, Wander; Barlow, Roger; Barschel, Colin; Barsuk, Sergey; Barter, William; Batozskaya, Varvara; Battista, Vincenzo; Bay, Aurelio; Beaucourt, Leo; Beddow, John; Bedeschi, Franco; Bediaga, Ignacio; Belogurov, Sergey; Belous, Konstantin; Belyaev, Ivan; Ben-Haim, Eli; Bencivenni, Giovanni; Benson, Sean; Benton, Jack; Berezhnoy, Alexander; Bernet, Roland; Bettler, Marc-Olivier; van Beuzekom, Martinus; Bien, Alexander; Bifani, Simone; Bird, Thomas; Bizzeti, Andrea; Bjørnstad, Pål Marius; Blake, Thomas; Blanc, Frédéric; Blouw, Johan; Blusk, Steven; Bocci, Valerio; Bondar, Alexander; Bondar, Nikolay; Bonivento, Walter; Borghi, Silvia; Borgia, Alessandra; Borsato, Martino; Bowcock, Themistocles; Bowen, Espen Eie; Bozzi, Concezio; Brambach, Tobias; van den Brand, Johannes; Bressieux, Joël; Brett, David; Britsch, Markward; Britton, Thomas; Brodzicka, Jolanta; Brook, Nicholas; Brown, Henry; Bursche, Albert; Busetto, Giovanni; Buytaert, Jan; Cadeddu, Sandro; Calabrese, Roberto; Calvi, Marta; Calvo Gomez, Miriam; Campana, Pierluigi; Campora Perez, Daniel; Carbone, Angelo; Carboni, Giovanni; Cardinale, Roberta; Cardini, Alessandro; Carson, Laurence; Carvalho Akiba, Kazuyoshi; Casse, Gianluigi; Cassina, Lorenzo; Castillo Garcia, Lucia; Cattaneo, Marco; Cauet, Christophe; Cenci, Riccardo; Charles, Matthew; Charpentier, Philippe; Chefdeville, Maximilien; Chen, Shanzhen; Cheung, Shu-Faye; Chiapolini, Nicola; Chrzaszcz, Marcin; Ciba, Krzystof; Cid Vidal, Xabier; Ciezarek, Gregory; Clarke, Peter; Clemencic, Marco; Cliff, Harry; Closier, Joel; Coco, Victor; Cogan, Julien; Cogneras, Eric; Cojocariu, Lucian; Collins, Paula; Comerma-Montells, Albert; Contu, Andrea; Cook, Andrew; Coombes, Matthew; Coquereau, Samuel; Corti, Gloria; Corvo, Marco; Counts, Ian; Couturier, Benjamin; Cowan, Greig; Craik, Daniel Charles; Cruz Torres, Melissa Maria; Cunliffe, Samuel; Currie, Robert; D'Ambrosio, Carmelo; Dalseno, Jeremy; David, Pascal; David, Pieter; Davis, Adam; De Bruyn, Kristof; De Capua, Stefano; De Cian, Michel; De Miranda, Jussara; De Paula, Leandro; De Silva, Weeraddana; De Simone, Patrizia; Decamp, Daniel; Deckenhoff, Mirko; Del Buono, Luigi; Déléage, Nicolas; Derkach, Denis; Deschamps, Olivier; Dettori, Francesco; Di Canto, Angelo; Dijkstra, Hans; Donleavy, Stephanie; Dordei, Francesca; Dorigo, Mirco; Dosil Suárez, Alvaro; Dossett, David; Dovbnya, Anatoliy; Dreimanis, Karlis; Dujany, Giulio; Dupertuis, Frederic; Durante, Paolo; Dzhelyadin, Rustem; Dziurda, Agnieszka; Dzyuba, Alexey; Easo, Sajan; Egede, Ulrik; Egorychev, Victor; Eidelman, Semen; Eisenhardt, Stephan; Eitschberger, Ulrich; Ekelhof, Robert; Eklund, Lars; El Rifai, Ibrahim; Elsasser, Christian; Ely, Scott; Esen, Sevda; Evans, Hannah Mary; Evans, Timothy; Falabella, Antonio; Färber, Christian; Farinelli, Chiara; Farley, Nathanael; Farry, Stephen; Fay, Robert; Ferguson, Dianne; Fernandez Albor, Victor; Ferreira Rodrigues, Fernando; Ferro-Luzzi, Massimiliano; Filippov, Sergey; Fiore, Marco; Fiorini, Massimiliano; Firlej, Miroslaw; Fitzpatrick, Conor; Fiutowski, Tomasz; Fontana, Marianna; Fontanelli, Flavio; Forty, Roger; Francisco, Oscar; Frank, Markus; Frei, Christoph; Frosini, Maddalena; Fu, Jinlin; Furfaro, Emiliano; Gallas Torreira, Abraham; Galli, Domenico; Gallorini, Stefano; Gambetta, Silvia; Gandelman, Miriam; Gandini, Paolo; Gao, Yuanning; García Pardiñas, Julián; Garofoli, Justin; Garra Tico, Jordi; Garrido, Lluis; Gaspar, Clara; Gauld, Rhorry; Gavardi, Laura; Gavrilov, Gennadii; Geraci, Angelo; Gersabeck, Evelina; Gersabeck, Marco; Gershon, Timothy; Ghez, Philippe; Gianelle, Alessio; Giani', Sebastiana; Gibson, Valerie; Giubega, Lavinia-Helena; Gligorov, Vladimir; Göbel, Carla; Golubkov, Dmitry; Golutvin, Andrey; Gomes, Alvaro; Gotti, Claudio; Grabalosa Gándara, Marc; Graciani Diaz, Ricardo; Granado Cardoso, Luis Alberto; Graugés, Eugeni; Graziani, Giacomo; Grecu, Alexandru; Greening, Edward; Gregson, Sam; Griffith, Peter; Grillo, Lucia; Grünberg, Oliver; Gui, Bin; Gushchin, Evgeny; Guz, Yury; Gys, Thierry; Hadjivasiliou, Christos; Haefeli, Guido; Haen, Christophe; Haines, Susan; Hall, Samuel; Hamilton, Brian; Hampson, Thomas; Han, Xiaoxue; Hansmann-Menzemer, Stephanie; Harnew, Neville; Harnew, Samuel; Harrison, Jonathan; He, Jibo; Head, Timothy; Heijne, Veerle; Hennessy, Karol; Henrard, Pierre; Henry, Louis; Hernando Morata, Jose Angel; van Herwijnen, Eric; Heß, Miriam; Hicheur, Adlène; Hill, Donal; Hoballah, Mostafa; Hombach, Christoph; Hulsbergen, Wouter; Hunt, Philip; Hussain, Nazim; Hutchcroft, David; Hynds, Daniel; Idzik, Marek; Ilten, Philip; Jacobsson, Richard; Jaeger, Andreas; Jalocha, Pawel; Jans, Eddy; Jaton, Pierre; Jawahery, Abolhassan; Jing, Fanfan; John, Malcolm; Johnson, Daniel; Jones, Christopher; Joram, Christian; Jost, Beat; Jurik, Nathan; Kaballo, Michael; Kandybei, Sergii; Kanso, Walaa; Karacson, Matthias; Karbach, Moritz; Karodia, Sarah; Kelsey, Matthew; Kenyon, Ian; Ketel, Tjeerd; Khanji, Basem; Khurewathanakul, Chitsanu; Klaver, Suzanne; Klimaszewski, Konrad; Kochebina, Olga; Kolpin, Michael; Komarov, Ilya; Koopman, Rose; Koppenburg, Patrick; Korolev, Mikhail; Kozlinskiy, Alexandr; Kravchuk, Leonid; Kreplin, Katharina; Kreps, Michal; Krocker, Georg; Krokovny, Pavel; Kruse, Florian; Kucewicz, Wojciech; Kucharczyk, Marcin; Kudryavtsev, Vasily; Kurek, Krzysztof; Kvaratskheliya, Tengiz; La Thi, Viet Nga; Lacarrere, Daniel; Lafferty, George; Lai, Adriano; Lambert, Dean; Lambert, Robert W; Lanfranchi, Gaia; Langenbruch, Christoph; Langhans, Benedikt; Latham, Thomas; Lazzeroni, Cristina; Le Gac, Renaud; van Leerdam, Jeroen; Lees, Jean-Pierre; Lefèvre, Regis; Leflat, Alexander; Lefrançois, Jacques; Leo, Sabato; Leroy, Olivier; Lesiak, Tadeusz; Leverington, Blake; Li, Yiming; Likhomanenko, Tatiana; Liles, Myfanwy; Lindner, Rolf; Linn, Christian; Lionetto, Federica; Liu, Bo; Lohn, Stefan; Longstaff, Iain; Lopes, Jose; Lopez-March, Neus; Lowdon, Peter; Lu, Haiting; Lucchesi, Donatella; Luo, Haofei; Lupato, Anna; Luppi, Eleonora; Lupton, Oliver; Machefert, Frederic; Machikhiliyan, Irina V; Maciuc, Florin; Maev, Oleg; Malde, Sneha; Malinin, Alexander; Manca, Giulia; Mancinelli, Giampiero; Mapelli, Alessandro; Maratas, Jan; Marchand, Jean François; Marconi, Umberto; Marin Benito, Carla; Marino, Pietro; Märki, Raphael; Marks, Jörg; Martellotti, Giuseppe; Martens, Aurelien; Martín Sánchez, Alexandra; Martinelli, Maurizio; Martinez Santos, Diego; Martinez Vidal, Fernando; Martins Tostes, Danielle; Massafferri, André; Matev, Rosen; Mathe, Zoltan; Matteuzzi, Clara; Mazurov, Alexander; McCann, Michael; McCarthy, James; McNab, Andrew; McNulty, Ronan; McSkelly, Ben; Meadows, Brian; Meier, Frank; Meissner, Marco; Merk, Marcel; Milanes, Diego Alejandro; Minard, Marie-Noelle; Moggi, Niccolò; Molina Rodriguez, Josue; Monteil, Stephane; Morandin, Mauro; Morawski, Piotr; Mordà, Alessandro; Morello, Michael Joseph; Moron, Jakub; Morris, Adam Benjamin; Mountain, Raymond; Muheim, Franz; Müller, Katharina; Mussini, Manuel; Muster, Bastien; Naik, Paras; Nakada, Tatsuya; Nandakumar, Raja; Nasteva, Irina; Needham, Matthew; Neri, Nicola; Neubert, Sebastian; Neufeld, Niko; Neuner, Max; Nguyen, Anh Duc; Nguyen, Thi-Dung; Nguyen-Mau, Chung; Nicol, Michelle; Niess, Valentin; Niet, Ramon; Nikitin, Nikolay; Nikodem, Thomas; Novoselov, Alexey; O'Hanlon, Daniel Patrick; Oblakowska-Mucha, Agnieszka; Obraztsov, Vladimir; Oggero, Serena; Ogilvy, Stephen; Okhrimenko, Oleksandr; Oldeman, Rudolf; Onderwater, Gerco; Orlandea, Marius; Otalora Goicochea, Juan Martin; Owen, Patrick; Oyanguren, Maria Arantza; Pal, Bilas Kanti; Palano, Antimo; Palombo, Fernando; Palutan, Matteo; Panman, Jacob; Papanestis, Antonios; Pappagallo, Marco; Pappalardo, Luciano; Parkes, Christopher; Parkinson, Christopher John; Passaleva, Giovanni; Patel, Girish; Patel, Mitesh; Patrignani, Claudia; Pazos Alvarez, Antonio; Pearce, Alex; Pellegrino, Antonio; Pepe Altarelli, Monica; Perazzini, Stefano; Perez Trigo, Eliseo; Perret, Pascal; Perrin-Terrin, Mathieu; Pescatore, Luca; Pesen, Erhan; Petridis, Konstantin; Petrolini, Alessandro; Picatoste Olloqui, Eduardo; Pietrzyk, Boleslaw; Pilař, Tomas; Pinci, Davide; Pistone, Alessandro; Playfer, Stephen; Plo Casasus, Maximo; Polci, Francesco; Poluektov, Anton; Polycarpo, Erica; Popov, Alexander; Popov, Dmitry; Popovici, Bogdan; Potterat, Cédric; Price, Eugenia; Prisciandaro, Jessica; Pritchard, Adrian; Prouve, Claire; Pugatch, Valery; Puig Navarro, Albert; Punzi, Giovanni; Qian, Wenbin; Rachwal, Bartolomiej; Rademacker, Jonas; Rakotomiaramanana, Barinjaka; Rama, Matteo; Rangel, Murilo; Raniuk, Iurii; Rauschmayr, Nathalie; Raven, Gerhard; Reichert, Stefanie; Reid, Matthew; dos Reis, Alberto; Ricciardi, Stefania; Richards, Sophie; Rihl, Mariana; Rinnert, Kurt; Rives Molina, Vincente; Roa Romero, Diego; Robbe, Patrick; Rodrigues, Ana Barbara; Rodrigues, Eduardo; Rodriguez Perez, Pablo; Roiser, Stefan; Romanovsky, Vladimir; Romero Vidal, Antonio; Rotondo, Marcello; Rouvinet, Julien; Ruf, Thomas; Ruiz, Hugo; Ruiz Valls, Pablo; Saborido Silva, Juan Jose; Sagidova, Naylya; Sail, Paul; Saitta, Biagio; Salustino Guimaraes, Valdir; Sanchez Mayordomo, Carlos; Sanmartin Sedes, Brais; Santacesaria, Roberta; Santamarina Rios, Cibran; Santovetti, Emanuele; Sarti, Alessio; Satriano, Celestina; Satta, Alessia; Saunders, Daniel Martin; Savrie, Mauro; Savrina, Darya; Schiller, Manuel; Schindler, Heinrich; Schlupp, Maximilian; Schmelling, Michael; Schmidt, Burkhard; Schneider, Olivier; Schopper, Andreas; Schune, Marie Helene; Schwemmer, Rainer; Sciascia, Barbara; Sciubba, Adalberto; Seco, Marcos; Semennikov, Alexander; Sepp, Indrek; Serra, Nicola; Serrano, Justine; Sestini, Lorenzo; Seyfert, Paul; Shapkin, Mikhail; Shapoval, Illya; Shcheglov, Yury; Shears, Tara; Shekhtman, Lev; Shevchenko, Vladimir; Shires, Alexander; Silva Coutinho, Rafael; Simi, Gabriele; Sirendi, Marek; Skidmore, Nicola; Skwarnicki, Tomasz; Smith, Anthony; Smith, Edmund; Smith, Eluned; Smith, Jackson; Smith, Mark; Snoek, Hella; Sokoloff, Michael; Soler, Paul; Soomro, Fatima; Souza, Daniel; Souza De Paula, Bruno; Spaan, Bernhard; Sparkes, Ailsa; Spradlin, Patrick; Sridharan, Srikanth; Stagni, Federico; Stahl, Marian; Stahl, Sascha; Steinkamp, Olaf; Stenyakin, Oleg; Stevenson, Scott; Stoica, Sabin; Stone, Sheldon; Storaci, Barbara; Stracka, Simone; Straticiuc, Mihai; Straumann, Ulrich; Stroili, Roberto; Subbiah, Vijay Kartik; Sun, Liang; Sutcliffe, William; Swientek, Krzysztof; Swientek, Stefan; Syropoulos, Vasileios; Szczekowski, Marek; Szczypka, Paul; Szilard, Daniela; Szumlak, Tomasz; T'Jampens, Stephane; Teklishyn, Maksym; Tellarini, Giulia; Teubert, Frederic; Thomas, Christopher; Thomas, Eric; van Tilburg, Jeroen; Tisserand, Vincent; Tobin, Mark; Tolk, Siim; Tomassetti, Luca; Tonelli, Diego; Topp-Joergensen, Stig; Torr, Nicholas; Tournefier, Edwige; Tourneur, Stephane; Tran, Minh Tâm; Tresch, Marco; Tsaregorodtsev, Andrei; Tsopelas, Panagiotis; Tuning, Niels; Ubeda Garcia, Mario; Ukleja, Artur; Ustyuzhanin, Andrey; Uwer, Ulrich; Vagnoni, Vincenzo; Valenti, Giovanni; Vallier, Alexis; Vazquez Gomez, Ricardo; Vazquez Regueiro, Pablo; Vázquez Sierra, Carlos; Vecchi, Stefania; Velthuis, Jaap; Veltri, Michele; Veneziano, Giovanni; Vesterinen, Mika; Viaud, Benoit; Vieira, Daniel; Vieites Diaz, Maria; Vilasis-Cardona, Xavier; Vollhardt, Achim; Volyanskyy, Dmytro; Voong, David; Vorobyev, Alexey; Vorobyev, Vitaly; Voß, Christian; Voss, Helge; de Vries, Jacco; Waldi, Roland; Wallace, Charlotte; Wallace, Ronan; Walsh, John; Wandernoth, Sebastian; Wang, Jianchun; Ward, David; Watson, Nigel; Websdale, David; Whitehead, Mark; Wicht, Jean; Wiedner, Dirk; Wilkinson, Guy; Williams, Matthew; Williams, Mike; Wilson, Fergus; Wimberley, Jack; Wishahi, Julian; Wislicki, Wojciech; Witek, Mariusz; Wormser, Guy; Wotton, Stephen; Wright, Simon; Wu, Suzhi; Wyllie, Kenneth; Xie, Yuehong; Xing, Zhou; Xu, Zhirui; Yang, Zhenwei; Yuan, Xuhao; Yushchenko, Oleg; Zangoli, Maria; Zavertyaev, Mikhail; Zhang, Liming; Zhang, Wen Chao; Zhang, Yanxi; Zhelezov, Alexey; Zhokhov, Anatoly; Zhong, Liang; Zvyagin, Alexander

2014-01-01

Measurements of the branching fractions of $B^0_{(s)} \\to K^{*\\pm}K^\\mp$ and $B^0_{(s)} \\to K^{*\\pm}\\pi^\\mp$ decays are performed using a data sample corresponding to $1.0 \\ {\\rm fb}^{-1}$ of proton-proton collision data collected with the LHCb detector at a centre-of-mass energy of $7\\mathrm{\\,TeV}$, where the $K^{*\\pm}$ mesons are reconstructed in the $K^0_{\\rm S}\\pi^\\pm$ final state. The first observation of the $B^0_s \\to K^{*\\pm}K^\\mp$ decay and the first evidence for the $B^0_s \\to K^{*-}\\pi^+$ decay are reported with branching fractions \\begin{eqnarray} {\\cal B}\\left(B^0_s \\to K^{*\\pm}K^\\mp\\right) & = & \\left( 12.7\\pm1.9\\pm1.9 \\right) \\times 10^{-6} \\, , \\\\ {\\cal B}\\left(B^0_s \\to K^{*-}\\pi^+\\right) & = & ~\\left( 3.3\\pm1.1\\pm0.5 \\right) \\times 10^{-6} \\, , \\end{eqnarray} where the first uncertainties are statistical and the second are systematic. In addition, an upper limit of ${\\cal B}\\left(B^0 \\to K^{*\\pm}K^\\mp\\right) < 0.4 \\ (0.5) \\times 10^{-6}$ is set at $90\\,\\% \\ (95\\,\\%)$ conf...

Corrosion characteristics of K-claddings

International Nuclear Information System (INIS)

Park, J. Y.; Choi, B. K.; Jung, Y. H.; Jung, Y. H.

2004-01-01

The Improvement of the corrosion resistance of nuclear fuel claddings is the critical issue for the successful development of the high burn-up fuel. KAERI have developed the K-claddings having a superior corrosion resistance by controlling the alloying element addition and optimizing the manufacturing process. The comparative evaluation of the corrosion resistance for K-claddings and the foreign claddings was performed and the effect of the heat treatment on the corrosion behavior of K-claddings was also examined. Corrosion tests were carried out in the conditions of 360 .deg. C pure water, PWR-simulating loop and 400 .deg. C steam, From the results of the corrosion tests, it was found that the corrosion resistance of K-claddings is superior to those of Zry4 and A claddings and K6 showed a better corrosion resistance than K3. The corrosion behavior of K-cladding was strongly influenced by the final annealing rather than the intermediate annealing, and the corrosion resistance increased with decreasing the final annealing temperature

Construction of testing facilities and verifying tests of a 22.9 kV/630 A class superconducting fault current limiter

Science.gov (United States)

Yim, S.-W.; Yu, S.-D.; Kim, H.-R.; Kim, M.-J.; Park, C.-R.; Yang, S.-E.; Kim, W.-S.; Hyun, O.-B.; Sim, J.; Park, K.-B.; Oh, I.-S.

2010-11-01

We have constructed and completed the preparation for a long-term operation test of a superconducting fault current limiter (SFCL) in a Korea Electric Power Corporation (KEPCO) test grid. The SFCL with rating of 22.9 kV/630 A, 3-phases, has been connected to the 22.9 kV test grid equipped with reclosers and other protection devices in Gochang Power Testing Center of KEPCO. The main goals of the test are the verification of SFCL performance and protection coordination studies. A line-commutation type SFCL was fabricated and installed for this project, and the superconducting components were cooled by a cryo-cooler to 77 K in the sub-cooled liquid nitrogen pressurized by 3 bar of helium gas. The verification test includes un-manned - long-term operation with and without loads and fault tests. Since the test site is 170 km away from the laboratory, we will adopt the un-manned operation with real-time remote monitoring and controlling using high speed internet. For the fault tests, we will apply fault currents up to around 8 kArms to the SFCL using an artificial fault generator. The fault tests may allow us not only to confirm the current limiting capability of the SFCL, but also to adjust the SFCL - recloser coordination such as resetting over-current relay parameters. This paper describes the construction of the testing facilities and discusses the plans for the verification tests.

Construction of testing facilities and verifying tests of a 22.9 kV/630 A class superconducting fault current limiter

International Nuclear Information System (INIS)

Yim, S.-W.; Yu, S.-D.; Kim, H.-R.; Kim, M.-J.; Park, C.-R.; Yang, S.-E.; Kim, W.-S.; Hyun, O.-B.; Sim, J.; Park, K.-B.; Oh, I.-S.

2010-01-01

We have constructed and completed the preparation for a long-term operation test of a superconducting fault current limiter (SFCL) in a Korea Electric Power Corporation (KEPCO) test grid. The SFCL with rating of 22.9 kV/630 A, 3-phases, has been connected to the 22.9 kV test grid equipped with reclosers and other protection devices in Gochang Power Testing Center of KEPCO. The main goals of the test are the verification of SFCL performance and protection coordination studies. A line-commutation type SFCL was fabricated and installed for this project, and the superconducting components were cooled by a cryo-cooler to 77 K in the sub-cooled liquid nitrogen pressurized by 3 bar of helium gas. The verification test includes un-manned - long-term operation with and without loads and fault tests. Since the test site is 170 km away from the laboratory, we will adopt the un-manned operation with real-time remote monitoring and controlling using high speed internet. For the fault tests, we will apply fault currents up to around 8 kA rms to the SFCL using an artificial fault generator. The fault tests may allow us not only to confirm the current limiting capability of the SFCL, but also to adjust the SFCL - recloser coordination such as resetting over-current relay parameters. This paper describes the construction of the testing facilities and discusses the plans for the verification tests.

9 CFR 590.547 - Albumen flake process drying operations.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Albumen flake process drying operations. 590.547 Section 590.547 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... free of flies, insects, and rodents. (b) Drying units, racks, and trucks shall be kept in a clean and...

Exon resequencing of H3K9 methyltransferase complex genes, EHMT1, EHTM2 and WIZ, in Japanese autism subjects.

Science.gov (United States)

Balan, Shabeesh; Iwayama, Yoshimi; Maekawa, Motoko; Toyota, Tomoko; Ohnishi, Tetsuo; Toyoshima, Manabu; Shimamoto, Chie; Esaki, Kayoko; Yamada, Kazuo; Iwata, Yasuhide; Suzuki, Katsuaki; Ide, Masayuki; Ota, Motonori; Fukuchi, Satoshi; Tsujii, Masatsugu; Mori, Norio; Shinkai, Yoichi; Yoshikawa, Takeo

2014-01-01

Histone H3 methylation at lysine 9 (H3K9) is a conserved epigenetic signal, mediating heterochromatin formation by trimethylation, and transcriptional silencing by dimethylation. Defective GLP (Ehmt1) and G9a (Ehmt2) histone lysine methyltransferases, involved in mono and dimethylation of H3K9, confer autistic phenotypes and behavioral abnormalities in animal models. Moreover, EHMT1 loss of function results in Kleefstra syndrome, characterized by severe intellectual disability, developmental delays and psychiatric disorders. We examined the possible role of histone methyltransferases in the etiology of autism spectrum disorders (ASD) and suggest that rare functional variants in these genes that regulate H3K9 methylation may be associated with ASD. Since G9a-GLP-Wiz forms a heteromeric methyltransferase complex, all the protein-coding regions and exon/intron boundaries of EHMT1, EHMT2 and WIZ were sequenced in Japanese ASD subjects. The detected variants were prioritized based on novelty and functionality. The expression levels of these genes were tested in blood cells and postmortem brain samples from ASD and control subjects. Expression of EHMT1 and EHMT2 isoforms were determined by digital PCR. We identified six nonsynonymous variants: three in EHMT1, two in EHMT2 and one in WIZ. Two variants, the EHMT1 ankyrin repeat domain (Lys968Arg) and EHMT2 SET domain (Thr961Ile) variants were present exclusively in cases, but showed no statistically significant association with ASD. The EHMT2 transcript expression was significantly elevated in the peripheral blood cells of ASD when compared with control samples; but not for EHMT1 and WIZ. Gene expression levels of EHMT1, EHMT2 and WIZ in Brodmann area (BA) 9, BA21, BA40 and the dorsal raphe nucleus (DoRN) regions from postmortem brain samples showed no significant changes between ASD and control subjects. Nor did expression levels of EHMT1 and EHMT2 isoforms in the prefrontal cortex differ significantly between ASD and

Study of the process e+e- → K+K- in the center-of-mass energy range 1010-1060 MeV with the CMD-3 detector

Science.gov (United States)

Kozyrev, E. A.; Solodov, E. P.; Akhmetshin, R. R.; Amirkhanov, A. N.; Anisenkov, A. V.; Aulchenko, V. M.; Banzarov, V. S.; Bashtovoy, N. S.; Berkaev, D. E.; Bondar, A. E.; Bragin, A. V.; Eidelman, S. I.; Epifanov, D. A.; Epshteyn, L. B.; Erofeev, A. L.; Fedotovich, G. V.; Gayazov, S. E.; Grebenuk, A. A.; Gribanov, S. S.; Grigoriev, D. N.; Ignatov, F. V.; Ivanov, V. L.; Karpov, S. V.; Kasaev, A. S.; Kazanin, V. F.; Korobov, A. A.; Koop, I. A.; Kozyrev, A. N.; Krokovny, P. P.; Kuzmenko, A. E.; Kuzmin, A. S.; Logashenko, I. B.; Lukin, P. A.; Lysenko, A. P.; Mikhailov, K. Yu.; Okhapkin, V. S.; Perevedentsev, E. A.; Pestov, Yu. N.; Popov, A. S.; Razuvaev, G. P.; Rogovsky, Yu. A.; Ruban, A. A.; Ryskulov, N. M.; Ryzhenenkov, A. E.; Shebalin, V. E.; Shemyakin, D. N.; Shwartz, B. A.; Shwartz, D. B.; Sibidanov, A. L.; Shatunov, Yu. M.; Talyshev, A. A.; Vorobiov, A. I.; Yudin, Yu. V.

2018-04-01

The process e+e- →K+K- has been studied using 1.7 ×106 events from a data sample corresponding to an integrated luminosity of 5.7 pb-1 collected with the CMD-3 detector in the center-of-mass energy range 1010-1060 MeV. The cross section is measured with about 2% systematic uncertainty and is used to calculate the contribution to the anomalous magnetic moment of the muon aμK+K- = (19.33 ± 0.40) ×10-10, and to obtain the ϕ (1020) meson parameters. We consider the relationship between the e+e- →K+K- and e+e- → KS0 KL0 cross sections and compare it to the theoretical prediction.

48 CFR 52.249-9 - Default (Fixed-Price Research and Development).

Science.gov (United States)

2010-10-01

... Research and Development). 52.249-9 Section 52.249-9 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.249-9 Default (Fixed-Price Research and Development). As prescribed in 49.504(b), insert the following clause: Default (Fixed-Price Research and Development) (APR 1984) (a)(1) The...

Synthesis and characterization of K{sub 2}UP{sub 3}Se{sub 9}. The first actinide seleno-phosphate

Energy Technology Data Exchange (ETDEWEB)

Chondroudis, K; Kanatzidis, M G [Michigan State Univ., East Lansing, MI (United States). Dept. of Chemistry

1997-12-31

The reaction of U with a molten mixture of K{sub 2}Se/P{sub 2}Se{sub 5}/Se at 490 deg. C produced the quaternary compound K{sub 2}UP{sub 3}Se{sub 9} as black, air stable crystals. It crystallizes in the triclinic space group P - 1 with a = 10.407(3) angstrom, b = 16.491(7) angstrom, c = 10.143(3) angstrom, {alpha} 107.51(3) deg., {beta} = 91.74(2) deg., {gamma} = 90.28(3) deg., Z = 4. The compound features an elaborate lamellar structure made of (U{sub 2}Se{sub 14}){sub x} columns linked in two dimensions by the [P{sub 2}Se{sub 6}]{sup 4-} anions. The compound melts congruently at 573 deg. C. The majestic susceptibility data are consistent with strong paramagnetic behaviour, conforming to Curie-Weiss law, above 100 K, whereas below this temperature a transition occurs followed antiferromagnetic ordering. (authors). 15 refs., 5 figs., 2 tabs.

Investigation and development of the suppression methods of the {sup 42}K background in LArGe

Energy Technology Data Exchange (ETDEWEB)

Lubashevskiy, Alexey [Max-Planck-Institut fuer Kernphysik, Saupfercheckweg 1, D-69117 Heidelberg (Germany); Collaboration: GERDA-Collaboration

2013-07-01

GERDA is an ultra-low background experiment aimed for the neutrinoless double beta decay search. The search is performed using HPGe detectors operated in liquid argon (LAr). One of the most dangerous backgrounds in GERDA is the background from {sup 42}K which is a daughter isotope of cosmogenically produced {sup 42}Ar. {sup 42}K ions are collected towards to the detector by the electric field of the detector. Estimation of the background contribution and development of the suppression methods were performed in the low background test facility LArGe. For this purpose encapsulated HPGe and bare BEGe detectors were operated in 1m{sup 3} of LAr in the LArGe setup. It is equipped with scintillation veto, so particles which deposit part of their energy in LAr can be detected by 9 PMTs. In order to better understand background and to increase statistics the LAr of LArGe was spiked with specially produced {sup 42}Ar. All these investigations allowed us to estimate background contribution from {sup 42}K and demonstrate the possibility to suppress it in future measurements in GERDA Phase II.

Recent development in thermally activated desalination methods: achieving an energy efficiency less than 2.5 kWhelec/m3

KAUST Repository

Shahzad, Muhammad Wakil

2015-05-19

Water-Energy-Environment nexus is a crucial consideration when designing seawater desalination processes, particularly for the water-stressed countries where the annual water availability is less than 250 m3 per capita. Despite the thermodynamics limit for seawater desalination at normal conditions is about 0.78 to 1.09 kWhelec/m3, the specific energy consumption of desalination of real plants is found to operate at several folds higher. Today’s technological advancement in membranes, namely the reverse osmosis processes, has set an energy consumption of around 3.5–5 kWhelec/m3, while the conventional perception of thermally activated processes such as MSF and MED tends to be higher. Although the higher energetic specific consumption of MED or MSF processes appeared to be higher at 60–100 kWhthermal/m3, their true electricity equivalent has been converted, hitherto, using the energetic analyses where the work potential of working steam of the processes cannot be captured adequately. Thermally activated processes, such as MED and MSF, form the bottoming cycle of a cogeneration plant where both electricity and desalination processes operate in tandem in a cascaded manner. Only the bled-steam at lower exergy is extracted for the desalination processes. In this presentation, we demonstrate that in a cogen plant with 30% bled-steam for MED processes, the exergy destruction ratio is found to be less than 7% of the total available exergy that emanated from the boilers. By the exergetic approach, the equivalent electricity consumption of an average 75 kWhthermal/m3 would result in an electrical equivalent of less than 2.5 kWhelec/m3. Also in this presentation, the authors will elaborate the latest developments in the use of hybridization concept where the MED and the AD cycles are thermodynamically integrated and enhancing the overall efficiency of desalination. © 2015 Balaban Desalination Publications. All rights reserved.

Cross Sections for the Reactions e+e to K+ K- pi+pi-, K+ K- pi0pi0, and K+ K- K+ K- Measured Using Initial-State Radiation

Energy Technology Data Exchange (ETDEWEB)

Lees, J.P.; Poireau, V.; Prencipe, E.; Tisserand, V.; /Annecy, LAPP; Garra Tico, J.; Grauges, E.; /Barcelona U., ECM; Martinelli, M.; /INFN, Trieste /Trieste U.; Milanes, D.A.; /INFN, Trieste /Trieste U.; Palano, A.; /INFN, Trieste /Trieste U.; Pappagallo, M.; /INFN, Trieste /Trieste U. /INFN, Bari /Bari U. /Bari U. /INFN, Bari; Eigen, G.; Stugu, B.; Sun, L.; /Bergen U.; Brown, D.N.; Kerth, L.T.; Kolomensky, Yu.G.; Lynch, G.; Osipenkov, I.L.; /LBL, Berkeley /UC, Berkeley; Koch, H.; Schroeder, T.; /Ruhr U., Bochum; Asgeirsson, D.J.; /British Columbia U. /Brunel U. /Novosibirsk, IYF /UC, Irvine /UC, Riverside /UC, Santa Barbara /UC, Santa Cruz /Caltech /Cincinnati U. /Colorado U. /Colorado State U. /Dortmund U. /Dresden, Tech. U. /Ecole Polytechnique /Edinburgh U. /INFN, Trieste /INFN, Trieste /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /INFN, Ferrara /Ferrara U. /Frascati /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /INFN, Trieste /Trieste U. /INFN, Trieste /INFN, Genoa /Genoa U. /Indian Inst. Tech., Guwahati /Harvard U. /Harvey Mudd Coll. /Heidelberg U. /Humboldt U., Berlin /Imperial Coll., London /Iowa State U. /Iowa State U. /Johns Hopkins U. /Orsay, LAL /LLNL, Livermore /Liverpool U. /Queen Mary, U. of London /Royal Holloway, U. of London /Louisville U. /Mainz U., Inst. Kernphys. /Manchester U., Comp. Sci. Dept. /Maryland U. /Massachusetts U., Amherst /MIT /McGill U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Milan /Milan U. /Mississippi U. /Montreal U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Naples /Naples U. /NIKHEF, Amsterdam /Notre Dame U. /Ohio State U. /Oregon U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /INFN, Trieste /INFN, Trieste /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Padua /Padua U. /Paris U., VI-VII /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Perugia /Perugia U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /INFN, Pisa /Princeton U. /INFN, Trieste /INFN, Trieste /INFN, Trieste /Trieste U. /INFN, Trieste /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /INFN, Trieste /INFN, Trieste /INFN, Rome /Rome U. /Rostock U. /Rutherford /DAPNIA, Saclay /SLAC /South Carolina U. /Southern Methodist U. /Stanford U., Phys. Dept. /SUNY, Albany /Tel Aviv U. /Tennessee U. /Texas U. /Texas U., Dallas /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Turin /Turin U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /INFN, Trieste /Trieste U. /Valencia U., IFIC /Victoria U. /Warwick U. /Wisconsin U., Madison

2011-08-19

We study the processes e{sup +}e{sup -} {yields} K{sup +}K{sup -}{pi}{sup +}{pi}-{gamma}, K{sup +}K{sup -}{pi}{sup 0}{pi}{sup 0}{gamma}, and K{sup +}K{sup -}K{sup +}K{sup -}{gamma}, where the photon is radiated from the initial state. About 84000, 8000, and 4200 fully reconstructed events, respectively, are selected from 454 fb{sup -1} of BABAR data. The invariant mass of the hadronic final state defines the e{sup +}e{sup -} center-of-mass energy, so that the K{sup +}K{sup -}{pi}{sup +}{pi}{sup -}{gamma} data can be compared with direct measurements of the e{sup +}e{sup -} {yields} K{sup +}K{sup -}{pi}{sup +}{pi}{sup -} reaction. No direct measurements exist for the e{sup +}e{sup -} {yields} K{sup +}K{sup -}{pi}{sup 0}{pi}{sup 0} or e{sup +}e{sup -} {yields} K{sup +}K{sup -}K{sup +}K{sup -} reactions, and we present an update of our previous result with doubled statistics. Studying the structure of these events, we find contributions from a number of intermediate states, and extract their cross sections. In particular, we perform a more detailed study of the e{sup +}e{sup -} {yields} {phi}(1020){pi}{pi}{gamma} reaction, and confirm the presence of the Y (2175) resonance in the {phi}(1020)f{sub 0}(980) and K{sup +}K{sup -} f{sub 0}(980) modes. In the charmonium region, we observe the J/{psi} in all three final states and in several intermediate states, as well as the {phi}(2S) in some modes, and measure the corresponding branching fractions.

The dynamics of Ca2+ ions within the solvation shell of calbindin D9k.

Directory of Open Access Journals (Sweden)

Elad Project

Full Text Available The encounter of a Ca(2+ ion with a protein and its subsequent binding to specific binding sites is an intricate process that cannot be fully elucidated from experimental observations. We have applied Molecular Dynamics to study this process with atomistic details, using Calbindin D9k (CaB as a model protein. The simulations show that in most of the time the Ca(2+ ion spends within the Debye radius of CaB, it is being detained at the 1st and 2nd solvation shells. While being detained near the protein, the diffusion coefficient of the ion is significantly reduced. However, due to the relatively long period of detainment, the ion can scan an appreciable surface of the protein. The enhanced propagation of the ion on the surface has a functional role: significantly increasing the ability of the ion to scan the protein's surface before being dispersed to the bulk. The contribution of this mechanism to Ca(2+ binding becomes significant at low ion concentrations, where the intervals between successive encounters with the protein are getting longer. The efficiency of the surface diffusion is affected by the distribution of charges on the protein's surface. Comparison of the Ca(2+ binding dynamics in CaB and its E60D mutant reveals that in the wild type (WT protein the carboxylate of E60 function as a preferred landing-site for the Ca(2+ arriving from the bulk, followed by delivering it to the final binding site. Replacement of the glutamate by aspartate significantly reduced the ability to transfer Ca(2+ ions from D60 to the final binding site, explaining the observed decrement in the affinity of the mutated protein to Ca(2+.

Annotation Method (AM): SE9_AM1 [Metabolonote[Archive

Lifescience Database Archive (English)

Full Text Available base search. Peaks with no hit to these databases are then selected to secondary se...arch using exactMassDB and Pep1000 databases. After the database search processes, each database hits are ma...SE9_AM1 PowerGet annotation A1 In annotation process, KEGG, KNApSAcK and LipidMAPS are used for primary data

Supersymmetric corrections to the K/sub L/ -> 2. gamma. process

Energy Technology Data Exchange (ETDEWEB)

Abad, J.; Rodriguez-Trias, R.

1988-11-01

An improved calculation of susy-corrections to the K/sub L/ -> 2..gamma.. process is done, including also the charged-Higgs contributions. The Higgsino-Wino mixings are carefully considered and the results are presented as a function of a susy-breaking parameter. An evaluation of the diagrams which include super-symmetric penguins is also done, finding a contribution somewhat lower than the previous one. We conclude that the Higgs and susy amplitudes are smaller than the standard amplitude.

Process development

International Nuclear Information System (INIS)

Zapata G, G.

1989-01-01

Process development: The paper describes the organization and laboratory facilities of the group working on radioactive ore processing studies. Contains a review of the carried research and the plans for the next future. A list of the published reports is also presented

Microwave source development for 9 MeV RF electron LINAC for cargo scanning

International Nuclear Information System (INIS)

Yadav, V.; Chandan, Shiv; Tillu, A.R.; Bhattacharjee, D.; Chavan, R.B.; Dixit, K.P.; Mittal, K.C.; Gantayet, L.M.

2011-01-01

For cargo scanning, high energy X-rays are required. These X-rays can be generated from accelerated electrons. A 9 MeV Cargo scanning RF LINAC has been developed at ECIL, Hyderabad. The Microwave power source required for RF Linac is a klystron-based system generating 5.5 MW peak, 10 kW average, at 2.856 GHz. Various components required for microwave source were identified, procured, tested and integrated into the source. Microwave source was tested on water load, then it was connected to LINAC and RF conditioning and e-beam trials were successfully done. For operating the microwave source, a PC based remote handling system was also designed and developed for operating various power supplies and instruments of the microwave source, including the Klystron modulator, Signal generator and other devices. The accelerator operates in pulse mode, requiring synchronous operation of the Klystron modulator, RF driver amplifier and E-gun modulator. For this purpose, a synchronous trigger generator was designed and developed. This paper describes the development and testing of microwave source and its remote operating system. The results of beam trials are also discussed in this paper. (author)

Investigation, modelling and control of the 1.9 K cooling loop for superconducting magnets for the Large Hadron Collider

CERN Document Server

Flemsæter, Bjorn

2000-01-01

The temperature of the superconducting magnets for the 27 km LHC particle accelerator under construction at CERN is a control parameter with strict operating constraints imposed by (a) the maximum temperature at which the magnets can operate, (b) the cooling capacity of the cryogenic system, (c) the variability of applied heat loads and (d) the accuracy of the instrumentation. A pilot plant for studying aspects beyond single magnet testing has been constructed. This magnet test string is a 35-m full-scale model if the LHC and consists of four superconducting cryogmagnets operating in a static bath of He II at 1.9 K. An experimental investigation of the properties dynamic characteristics of the 1.9 K cooling loop of the magnet test string has been carried out. A first principle model of the system has been created. A series of experiments designed for system identification purposes have been carried out, and black box models of the system have been created on the basis on the recorded data. A Model Predictive ...

32 CFR 516.9 - Service of criminal process within the United States.

Science.gov (United States)

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Service of criminal process within the United... OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Service of Process § 516.9 Service of criminal process within the United States. (a) Surrender of personnel. Guidance for surrender of military personnel...

Damage development in 9%Cr steels

International Nuclear Information System (INIS)

Rauch, M.; Maile, K.

2003-01-01

Modern 9-11% martensitic steels are candidate materials to be used in modern fossil fired power plants with high efficiency rates. The focus of the R and D work is put on the further development and optimisation, the determination of material characteristics but also on the identification and quantification of damage mechanisms and the damage evolution. For this purpose extensive experiments such as long creep tests on specimens under internal pressure, metallurgical examinations and theoretical investigations for determination of stress-strain state which have been conducted. The laboratory tests are completed by examination of real components. As a result an empirical description of the creep cavity density as a function of deformation and multiaxiality of stress state has been carried out which can be used in further FE-calculations determining the damage state. The results of all metallographical examinations on specimens with different heat treatments and service loads are summarised in a structure atlas and are published for further usage. Damage development, martensitic 9 % Cr steels, creep cavity density, creep tests under multiaxial load, metallographical investigations, and measurements on pipe bends. (author)

Circadian rhythm in concentrations of Mg, K, Ca and Mn in Japanese morning glory during flowering process

International Nuclear Information System (INIS)

Ikeue, Natsuko; Tanoi, Keitaro; Furukawa, Jun; Yokota, Harumi; Okuni, Yoko; Nakanishi, Tomoko M.

2001-01-01

Concentrations of 4 trace elements, Mg, K, Ca and Mn, in each tissues of Japanese morning glory were analyzed during the flower development. To determine the element amount, neutron activation analysis with gamma-ray spectroscopy was performed. In this study, we focused on the movement of the trace elements especially with short-day treatment. Each element showed its specific distribution in the parts of a plant. The concentration of each element was changed rhythmically within a day. It was noted that, in the apical bud, concentrations of Mg, Ca and Mn were decreased from 5 h (2 h before light was on) to 7 h and increased again after 9 h. (author)

Circadian rhythm in concentrations of Mg, K, Ca and Mn in Japanese morning glory during flowering process

Energy Technology Data Exchange (ETDEWEB)

Ikeue, Natsuko; Tanoi, Keitaro; Furukawa, Jun; Yokota, Harumi; Okuni, Yoko; Nakanishi, Tomoko M. [Tokyo Univ. (Japan). Graduate School of Agricultural and Life Sciences

2001-06-01

Concentrations of 4 trace elements, Mg, K, Ca and Mn, in each tissues of Japanese morning glory were analyzed during the flower development. To determine the element amount, neutron activation analysis with gamma-ray spectroscopy was performed. In this study, we focused on the movement of the trace elements especially with short-day treatment. Each element showed its specific distribution in the parts of a plant. The concentration of each element was changed rhythmically within a day. It was noted that, in the apical bud, concentrations of Mg, Ca and Mn were decreased from 5 h (2 h before light was on) to 7 h and increased again after 9 h. (author)

PENENTUAN DOSIS OPTIMUM PEMUPUKAN N, P, DAN K PADA SORGUM (SORGHUM BICOLOR [L.] MOENCH

Directory of Open Access Journals (Sweden)

Ratna Suminar

2017-04-01

Full Text Available Development of sorghum processing industries is essential in expanding sorghum market as wells as it’s an economic value. Fertilizer is needed in planting. Nitrogen, phosphorus, and potassium availabilities are the most limiting factors for maximum growth and yield. Sorghum (Numbu Variety was planted to evaluate the maximum and optimum rates of N, P, and K fertilizers. The research was conducted in Cikarawang field, Darmaga, Bogor from MarchJuly 2015 with multi-nutrient response method. Each experiment evaluated different rates of N, P, and K fertilizer with randomized complete block design and three replications. The fertilizer rates were 0, 50, 100, 150, and 200% of recommendation rate (100% N = 120 kg N ha-1, 100% P = 36 kg P2O5 ha-1, and 100% K = 90 kg K2O ha-1. The result showed relative yields of sorghum increased quadratically based on y = -0,0015x2 + 0,4011x + 67,571 for N, y = -0,0012x2 + 0,2917x + 78,457 for P2O5, and y = -0,001x2 + 0,2777x + 74,457 for K2O. The maximum rate for each nutrient was 160,4-43,7-124,9 kg N-P2O5-K2O ha-1. Fertilizer recommendation based on P threshold (no P was 36,7-0,0-13,7 kg N-P2O5-K2O ha-1, K threshold (no K was 22,0-0,0-0,0 kg N-P2O5-K2O ha-1, and no fertilizer needed on N threshold. The optimum rate fertilizer based on the yield vs. cost rule, therefore, the most economical recommendation would be 160,4-43,7-124,9 kg N-P2O5-K2O ha-1.

k c mistri

Indian Academy of Sciences (India)

Home; Journals; Sadhana. K C MISTRI. Articles written in Sadhana. Volume 42 Issue 9 September 2017 pp 1459-1471. Influence of yielding base and rigid base on propagation of Rayleigh-type wave in a viscoelastic layer of Voigt type · S SAHA A CHATTOPADHYAY K C MISTRI A K SINGH · More Details Abstract Fulltext ...

Development of a thermally switched superconducting rectifier for 100 kA

NARCIS (Netherlands)

Mulder, G.B.J.; Mulder, G.B.J.; ten Kate, Herman H.J.; Krooshoop, Hendrikus J.G.; van de Klundert, L.J.M.; van de Klundert, L.J.M.

1991-01-01

A full-wave superconducting rectifier for 100 kA has been developed. Typical design values of this device are: a secondary current of 100 kA, a primary amplitude of 20 A, an operating frequency of 0.5 Hz, and an average power on the order of 100 W. The rectification is achieved by means of thermally

Measurement of Multiple Vitamin K Forms in Processed and Fresh-Cut Pork Products in the U.S. Food Supply.

Science.gov (United States)

Fu, Xueyan; Shen, Xiaohua; Finnan, Emily G; Haytowitz, David B; Booth, Sarah L

2016-06-08

Vitamin K food composition data have historically been limited to plant-based phylloquinone (vitamin K1). The purpose of this study was to expand analysis of vitamin K to animal products and to measure phylloquinone and 10 forms of menaquinones (vitamin K2) in processed and fresh-cut pork products. Nationally representative samples of processed pork products (n = 28) were obtained through USDA's National Food and Nutrition Analysis Program, and fresh pork (six cuts; n = 5 per cut) and bacon (n = 4) were purchased from local retail outlets. All samples were analyzed by high-performance liquid chromatography (phylloquinone and menaquinone-4) and atmospheric-pressure chemical ionization-liquid chromatography-mass spectrometry (menaquinone-5 to menaquinone-13). Although low in phylloquinone (processed pork products and fresh pork cuts contained menaquinone-4, menaquinone-10, and menaquinone-11 (range: [35.1 ± 11.0]-[534 ± 89.0] μg of menaquinones per 100 g). The total menaquinone contents of processed pork products were correlated with fat contents (r = 0.935). In summary, processed and fresh-cut pork products are a rich dietary source of menaquinones that are currently unaccounted for in assessment of vitamin K in the food supply.

KEY COMPARISON: Final report on bilateral comparison CCEM.RF-K9.1: Thermal noise standards at 12.4 GHz, 13.5 GHz, 15 GHz and 17.5 GHz

Science.gov (United States)

Allal, Djamel

2009-01-01

A bilateral comparison subsequent to key comparison CCEM.RF-K9 was decided between VNIIFTRI and PTB. It was registered into the BIPM KCDB under the identifier CCEM.RF-K9.1. The results of this comparison were gathered by the pilot laboratory of comparison CCEM.RF-K9, the LNE, and a very good agreement was found between the two participants. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCEM, according to the provisions of the CIPM Mutual Recognition Arrangement (MRA).

Task 9. Photovoltaic deployment in developing countries. Financing mechanisms for solar home systems in developing countries. The role of financing in the dissemination process

Energy Technology Data Exchange (ETDEWEB)

Scheutzlich, T.; Pertz, K.; Klinghammer, W.; Scholand, M.; Wisniwski, S.

2002-09-15

This report for the International Energy Agency (IEA) made by Task 9 of the Photovoltaic Power Systems (PVPS) programme takes a look at the implementation of Solar Home Systems in developing countries. The objective of Task 9 is to increase the successful deployment of PV systems in developing countries. This paper takes a look at financing mechanisms for Solar Home Systems (SHS). The lack of financial services for users of SHS is often regarded as the main barrier for their commercial dissemination and is often the justification for donor assisted programmes. This study attempts to shed some light on the question whether commercial SHS dissemination in remote rural areas could be made easier if financial services were made available. The authors state that the thesis is based on the fact that carefully designed, target-group-oriented financial services may speed up the widespread dissemination of SHS. Financial mechanisms for the stimulation of SHS and how their commercialisation can be achieved are among the topics discussed.

Development of the Korean version of Alzheimer's Disease Assessment Scale (ADAS-K).

Science.gov (United States)

Youn, J C; Lee, D Y; Kim, K W; Lee, J H; Jhoo, J H; Lee, K U; Ha, J; Woo, J I

2002-09-01

The purpose of this study was the development of the Korean Version of Alzheimer's Disease Assessment Scale (ADAS-K). ADAS-K was administrated to 84 AD patients as well as 105 non-demented control subjects. Three aspects of reliability were tested. To evaluate the validity of ADAS-K, discriminant validity and concurrent validity were tested. To evaluate the sensitivity of ADAS-K to disease severity, all subjects, AD patients and control subjects, were grouped by CDR scale and their mean scores on ADAS-K were compared. ADAS-K demonstrated high levels of reliability. Mean ADAS-K scores for AD patients were significantly different from the control group (p ADAS-K exhibited significant correlations with other tests and scales (range 0.45-0.85, p ADAS-K displayed high diagnostic efficacy and the optimal cut-off point was selected between 18/19. ADAS-K was able to discriminate the degree of AD severity according to CDR classification. Our results suggested that ADAS-K-cog was sensitive to very mild AD. We demonstrated that ADAS-K is a reliable and valid instrument not only for AD diagnosis but also for evaluation of its severity. Copyright 2002 John Wiley & Sons, Ltd.

winged eye Induces Transdetermination of Drosophila Imaginal Disc by Acting in Concert with a Histone Methyltransferase, Su(var3-9

Directory of Open Access Journals (Sweden)

Keita Masuko

2018-01-01

Full Text Available Summary: Drosophila imaginal disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD. We previously identified winged eye (wge as a factor that induces eye-to-wing TD upon overexpression in eye imaginal discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9, a feature of heterochromatin. A histone methyltransferase, Su(var3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of imaginal disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var3-9 and activates TD-related genes by acting together with Su(var3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions. : Drosophila imaginal discs switch disc identity by a process known as transdetermination. Masuko et al. demonstrate that expression of the winged eye gene induces transdetermination through histone modifications such as H3K9-methylation. winged eye regulates expression of transdetermination-related genes via a histone methyltransferase, Su(var3-9. Keywords: Drosophila, imaginal disc, transdetermination, heterochromatin, cell fate, winged eye, reprogramming, Su(var3-9

Luminescence of YbP3O9 upon excitation in the UV-VUV range

International Nuclear Information System (INIS)

Stryganyuk, G; Trots, D; Berezovskaya, I; Shalapska, T; Voloshinovskii, A; Dotsenko, V; Zimmerer, G

2007-01-01

X-ray powder diffraction and luminescence spectral-kinetic studies have been performed for ytterbium metaphosphate (YbP 3 O 9 ) in the 12-290 K temperature range. The diffraction investigation has shown YbP 3 O 9 to be of monoclinic P 2 1 /c structure at T = 12-290 K. Charge transfer luminescence originating from Yb 3+ ion has been revealed. The carrier confinement within the Yb 3+ charge transfer state is pronounced at T = 12 K. A tendency has been revealed for charge carriers in YbP 3 O 9 to be localized in a trapped exciton state at room temperature. The quenching mechanisms for Yb 3+ charge transfer luminescence and processes competing with the formation of the Yb 3+ charge transfer state are discussed

Development of food preservation and processing techniques by radiation

International Nuclear Information System (INIS)

Byun, Myung Woo; Yook, Hong Sun; Lee, Ju Woon and others

2000-03-01

Development of food preservation and processing techniques by radiation was performed. Gamm irradiation at 5 kGy completely eliminated pathogenic bacteria in pork and chicken meats. Gamma irradiation at such doses and subsequent storage at less than 4 deg C could ensure hygienic quality and prolong the microbiological shelf-life resulting from the reduction of spoilage microorganisms. Pork loin ham with desirable color was also developed without using of sodium nitrite that is known as a carcinogen. Safety tests of gamma-irradiated meats in areas such as genotoxicity, acute toxicity, four-week oral toxicity, rat hepatocarcinogenesis and the antioxidative defense system, were not affected by gamma irradiation. Gamma irradiation at about 1 kGy completely eliminated the parasites in foods and drinking water. In the study of quarantine treatment of apple and pear for export by gamma irradiation, current fumigation(MBr) was perfect in its disinfesting capability, but it caused detrimental effects on the physical quality of apple and pear. However, irradiation doses at 1-3 kGy was suitable for controlling pests and did not induce any significant changes in the quality of the products. The result of the survey to assess the public understanding indicated that the irradiated food had somewhat negative impression to general public. Therefore, it is necessary to establish a public education and information program by using mass communication and by constructing communication system to obtain the enhanced impression from the general public

Development of food preservation and processing techniques by radiation

Energy Technology Data Exchange (ETDEWEB)

Byun, Myung Woo; Yook, Hong Sun; Lee, Ju Woon and others

2000-03-01

Development of food preservation and processing techniques by radiation was performed. Gamm irradiation at 5 kGy completely eliminated pathogenic bacteria in pork and chicken meats. Gamma irradiation at such doses and subsequent storage at less than 4 deg C could ensure hygienic quality and prolong the microbiological shelf-life resulting from the reduction of spoilage microorganisms. Pork loin ham with desirable color was also developed without using of sodium nitrite that is known as a carcinogen. Safety tests of gamma-irradiated meats in areas such as genotoxicity, acute toxicity, four-week oral toxicity, rat hepatocarcinogenesis and the antioxidative defense system, were not affected by gamma irradiation. Gamma irradiation at about 1 kGy completely eliminated the parasites in foods and drinking water. In the study of quarantine treatment of apple and pear for export by gamma irradiation, current fumigation(MBr) was perfect in its disinfesting capability, but it caused detrimental effects on the physical quality of apple and pear. However, irradiation doses at 1-3 kGy was suitable for controlling pests and did not induce any significant changes in the quality of the products. The result of the survey to assess the public understanding indicated that the irradiated food had somewhat negative impression to general public. Therefore, it is necessary to establish a public education and information program by using mass communication and by constructing communication system to obtain the enhanced impression from the general public.

K basins sludge removal sludge pretreatment system

International Nuclear Information System (INIS)

Chang, H.L.

1997-01-01

The Spent Nuclear Fuels Program is in the process of planning activities to remove spent nuclear fuel and other materials from the 100-K Basins as a remediation effort for clean closure. The 105 K- East and K-West Basins store spent fuel, sludge, and debris. Sludge has accumulated in the 1 00 K Basins as a result of fuel oxidation and a slight amount of general debris being deposited, by settling, in the basin water. The ultimate intent in removing the sludge and fuel is to eliminate the environmental risk posed by storing fuel at the K Basins. The task for this project is to disposition specific constituents of sludge (metallic fuel) to produce a product stream through a pretreatment process that will meet the requirements, including a final particle size acceptable to the Tank Waste Remediation System (TWRS). The purpose of this task is to develop a preconceptual design package for the K Basin sludge pretreatment system. The process equipment/system is at a preconceptual stage, as shown in sketch ES-SNF-01 , while a more refined process system and material/energy balances are ongoing (all sketches are shown in Appendix C). Thus, the overall process and 0535 associated equipment have been conservatively selected and sized, respectively, to establish the cost basis and equipment layout as shown in sketches ES- SNF-02 through 08

44 CFR Appendix A to Part 9 - Decision-making Process for E.O. 11988

Science.gov (United States)

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Decision-making Process for E.O. 11988 A Appendix A to Part 9 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT..., App. A Appendix A to Part 9—Decision-making Process for E.O. 11988 EC02FE91.074 ...

Decarboxylation of Δ 9-tetrahydrocannabinol: Kinetics and molecular modeling

Science.gov (United States)

Perrotin-Brunel, Helene; Buijs, Wim; van Spronsen, Jaap; van Roosmalen, Maaike J. E.; Peters, Cor J.; Verpoorte, Rob; Witkamp, Geert-Jan

2011-02-01

Efficient tetrahydrocannabinol (Δ 9-THC) production from cannabis is important for its medical application and as basis for the development of production routes of other drugs from plants. This work presents one of the steps of Δ 9-THC production from cannabis plant material, the decarboxylation reaction, transforming the Δ 9-THC-acid naturally present in the plant into the psychoactive Δ 9-THC. Results of experiments showed pseudo-first order reaction kinetics, with an activation barrier of 85 kJ mol -1 and a pre-exponential factor of 3.7 × 10 8 s -1. Using molecular modeling, two options were identified for an acid catalyzed β-keto acid type mechanism for the decarboxylation of Δ 9-THC-acid. Each of these mechanisms might play a role, depending on the actual process conditions. Formic acid proved to be a good model for a catalyst of such a reaction. Also, the computational idea of catalysis by water to catalysis by an acid, put forward by Li and Brill, and Churchev and Belbruno was extended, and a new direct keto-enol route was found. A direct keto-enol mechanism catalyzed by formic acid seems to be the best explanation for the observed activation barrier and the pre-exponential factor of the decarboxylation of Δ 9-THC-acid. Evidence for this was found by performing an extraction experiment with Cannabis Flos. It revealed the presence of short chain carboxylic acids supporting this hypothesis. The presented approach is important for the development of a sustainable production of Δ 9-THC from the plant.

9 CFR 381.304 - Operations in the thermal processing area.

Science.gov (United States)

2010-01-01

... AND VOLUNTARY INSPECTION AND CERTIFICATION POULTRY PRODUCTS INSPECTION REGULATIONS Canning and Canned... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Operations in the thermal processing area. 381.304 Section 381.304 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE...

Study of K. pi. scattering using the reactions K/sup + -/p. -->. K/sup + -/. pi. /sup +/n and K/sup + -/p. -->. K/sup + -/. pi. /sup -/. delta. /sup + +/ at 13 GeV/c

Energy Technology Data Exchange (ETDEWEB)

Estabrooks, P [McGill Univ., Montreal, Quebec (Canada); Carnegie, R K [Carleton Univ., Ottawa, Ontario (Canada); Martin, A D [Durham Univ. (UK); Dunwoodie, W M; Lasinski, T A; Leith, D W.G.S. [Stanford Linear Accelerator Center, Calif. (USA)

1978-02-27

An elastic K..pi.. partial-wave analysis is presented. It is based on high-statistics data for the reactions K/sup + -/p ..-->.. K/sup + -/..pi../sup +/n and K/sup + -/p ..-->.. K/sup + -/..pi../sup -/..delta../sup + +/ at 13 GeV obtained in a spectrometer experiment performed at SLAC. For each reaction, a t-dependent parametrization of the production amplitudes provides information on both the K..pi.. mass dependence of the production mechanisms and on K..pi.. scattering. Knowledge of the t-dependence then allows a calculation of the K..pi.. partial-wave amplitudes for K..pi.. masses from 0.7 to 1.9 GeV. The results of such analyses using data for (i) the neutral recoil reactions, (ii) the ..delta../sup + +/ recoil reactions, and (iii) both neutron and ..delta../sup + +/ recoil reactions simultaneously are presented.

Measurement of the branching ratio for the doubly cabibbo suppressed decay D₊ → ⁺ K^-K⁺K⁺; Medida da razao de ramificacao do Decaimento D⁺ → ⁺ K^-K⁺K⁺ duplamente suprimido por cabibbo

Energy Technology Data Exchange (ETDEWEB)

Silva Carvalho, Hendly da [Brazilian Centre for Physics Research (CBPF), Rio de Janeiro (Brazil).

1997-07-01

In this thesis, we performed a study for the decay modes D⁺ → ⁺ K^-K⁺K⁺ and D⁺_s → ⁺ K^-K⁺K⁺, using the data collected by the E791, a hadroproduction of charm experiment at Fermilab. The D⁺ → ⁺ K^-K⁺K⁺ decay is doubly Cabibbo suppressed while the D⁺_s → ⁺ K^-K⁺K⁺ decay is singly Cabibbo suppressed. We found 11.6 +- 3.9 events in the D⁺ mass region and 8.9 +- 3.3 in the D⁺_s mass region. The D⁺ → ⁺ K^-K⁺K⁺ branching ratio is measured to be (3.7 +- 1.3 +- 0.6) x 10^-4 while the D⁺ → ⁺ K^-K⁺K⁺ branching ratio relative to D⁺_s → ⁺ K^-K⁺K⁺ is measured to be (4.2 +- 1.5 +- 0.6) x 10^-2.

JMJ27, an Arabidopsis H3K9 histone demethylase, modulates defense against Pseudomonas syringae and flowering time.

Science.gov (United States)

Dutta, Aditya; Choudhary, Pratibha; Caruana, Julie; Raina, Ramesh

2017-09-01

Histone methylation is known to dynamically regulate diverse developmental and physiological processes. Histone methyl marks are written by methyltransferases and erased by demethylases, and result in modification of chromatin structure to repress or activate transcription. However, little is known about how histone methylation may regulate defense mechanisms and flowering time in plants. Here we report characterization of JmjC DOMAIN-CONTAINING PROTEIN 27 (JMJ27), an Arabidopsis JHDM2 (JmjC domain-containing histone demethylase 2) family protein, which modulates defense against pathogens and flowering time. JMJ27 is a nuclear protein containing a zinc-finger motif and a catalytic JmjC domain with conserved Fe(II) and α-ketoglutarate binding sites, and displays H3K9me1/2 demethylase activity both in vitro and in vivo. JMJ27 is induced in response to virulent Pseudomonas syringae pathogens and is required for resistance against these pathogens. JMJ27 is a negative modulator of WRKY25 (a repressor of defense) and a positive modulator of several pathogenesis-related (PR) proteins. Additionally, loss of JMJ27 function leads to early flowering. JMJ27 negatively modulates the major flowering regulator CONSTANS (CO) and positively modulates FLOWERING LOCUS C (FLC). Taken together, our results indicate that JMJ27 functions as a histone demethylase to modulate both physiological (defense) and developmental (flowering time) processes in Arabidopsis. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd.

Recent developments in the field of radiation processing

International Nuclear Information System (INIS)

Andrzej, G. Chmielewski

2006-01-01

Full text: Radiation has been discovered more than one hundred years ago. Since than, properties of radiation to modify physico-chemical properties of materials have found many applications. Radiation technologies applying gamma sources and electron accelerators for material processing are well established processes. There are over 160 gamma industrial irradiators and 1300 electron industrial accelerators in operation worldwide. They are being widely used for sterilization, food irradiation and polymer processing. New developments in the field of radiation sources engineering are compact size gamma irradiators, high power electron accelerators (medium energy range) for environmental applications and other types (high energy range) for materials' processing, with direct e-/X conversion. Future applications of low energy, inexpensive EB processing systems are foreseen. Electron beam lithography for microelectronics is a well-established technique. The already tested e-/X system equipped in an accelerator of 700 kW power opens new horizons for this kind of application. The developments described above need introduction of new computational methods that facilitate prediction of dose distribution, even in containers filled with complex products of varying densities. This technique provides good solutions for homeland security applications which may be complemented by mobile system applications. Technologies to be developed besides environmental applications could be nano materials, structure engineered materials (sorbents, the composites, ordered polymers, etc.) and natural polymers' processing. New products based on radiation processed polysaccharides have already been commercialized in many countries of the East Asia and Pacific Region, especially in those being rich in natural polymers. Very important and promising applications concern environment protection - radiation technology being a clean and environment friendly process, helps to curb pollutants' emission as

The structures of bacteriophages K1E and K1-5 explain processive degradation of polysaccharide capsules and evolution of new host specificities.

Science.gov (United States)

Leiman, Petr G; Battisti, Anthony J; Bowman, Valorie D; Stummeyer, Katharina; Mühlenhoff, Martina; Gerardy-Schahn, Rita; Scholl, Dean; Molineux, Ian J

2007-08-17

External polysaccharides of many pathogenic bacteria form capsules protecting the bacteria from the animal immune system and phage infection. However, some bacteriophages can digest these capsules using glycosidases displayed on the phage particle. We have utilized cryo-electron microscopy to determine the structures of phages K1E and K1-5 and thereby establish the mechanism by which these phages attain and switch their host specificity. Using a specific glycosidase, both phages penetrate the capsule and infect the neuroinvasive human pathogen Escherichia coli K1. In addition to the K1-specific glycosidase, each K1-5 particle carries a second enzyme that allows it to infect E. coli K5, whose capsule is chemically different from that of K1. The enzymes are organized into a multiprotein complex attached via an adapter protein to the virus portal vertex, through which the DNA is ejected during infection. The structure of the complex suggests a mechanism for the apparent processivity of degradation that occurs as the phage drills through the polysaccharide capsule. The enzymes recognize the adapter protein by a conserved N-terminal sequence, providing a mechanism for phages to acquire different enzymes and thus to evolve new host specificities.

SOX9 regulates ERBB signalling in pancreatic cancer development.

Science.gov (United States)

Grimont, Adrien; Pinho, Andreia V; Cowley, Mark J; Augereau, Cécile; Mawson, Amanda; Giry-Laterrière, Marc; Van den Steen, Géraldine; Waddell, Nicola; Pajic, Marina; Sempoux, Christine; Wu, Jianmin; Grimmond, Sean M; Biankin, Andrew V; Lemaigre, Frédéric P; Rooman, Ilse; Jacquemin, Patrick

2015-11-01

The transcription factor SOX9 was recently shown to stimulate ductal gene expression in pancreatic acinar-to-ductal metaplasia and to accelerate development of premalignant lesions preceding pancreatic ductal adenocarcinoma (PDAC). Here, we investigate how SOX9 operates in pancreatic tumourigenesis. We analysed genomic and transcriptomic data from surgically resected PDAC and extended the expression analysis to xenografts from PDAC samples and to PDAC cell lines. SOX9 expression was manipulated in human cell lines and mouse models developing PDAC. We found genetic aberrations in the SOX9 gene in about 15% of patient tumours. Most PDAC samples strongly express SOX9 protein, and SOX9 levels are higher in classical PDAC. This tumour subtype is associated with better patient outcome, and cell lines of this subtype respond to therapy targeting epidermal growth factor receptor (EGFR/ERBB1) signalling, a pathway essential for pancreatic tumourigenesis. In human PDAC, high expression of SOX9 correlates with expression of genes belonging to the ERBB pathway. In particular, ERBB2 expression in PDAC cell lines is stimulated by SOX9. Inactivating Sox9 expression in mice confirmed its role in PDAC initiation; it demonstrated that Sox9 stimulates expression of several members of the ERBB pathway and is required for ERBB signalling activity. By integrating data from patient samples and mouse models, we found that SOX9 regulates the ERBB pathway throughout pancreatic tumourigenesis. Our work opens perspectives for therapy targeting tumourigenic mechanisms. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Cadmium induces matrix metalloproteinase-9 expression via ROS-dependent EGFR, NF-kB, and AP-1 pathways in human endothelial cells

International Nuclear Information System (INIS)

Lian, Sen; Xia, Yong; Khoi, Pham Ngoc; Ung, Trong Thuan; Yoon, Hyun Joong; Kim, Nam Ho; Kim, Kyung Keun; Jung, Young Do

2015-01-01

Highlights: • Cadmium induces MMP-9 expression through NADPH oxidase-derived ROS. • Cadmium induces MMP-9 through EGFR-mediated Akt, Erk1/2 and JNK1/2 signaling pathways. • Akt, MAPKs (Erk1/2 and JNK1/2) functioned as upstream signals of NF-kB and AP-1 respectively, in cadmium-induced MMP-9 in endothelial cells. • ROS production by NADPH oxidase is the furthest upstream signal in MMP-9 expression in ECV304 cells. - Abstract: Cadmium (Cd), a widespread cumulative pollutant, is a known human carcinogen, associated with inflammation and tumors. Matrix metalloproteinase-9 (MMP-9) plays a pivotal role in tumor metastasis; however, the mechanisms underlying the MMP-9 expression induced by Cd remain obscure in human endothelial cells. Here, Cd elevated MMP-9 expression in dose- and time-dependent manners in human endothelial cells. Cd increased ROS production and the ROS-producing NADPH oxidase. Cd translocates p47 phox , a key subunit of NADPH oxidase, to the cell membrane. Cd also activated the phosphorylation of EGFR, Akt, Erk1/2, and JNK1/2 in addition to promoting NF-kB and AP-1 binding activities. Specific inhibitor and mutagenesis studies showed that EGFR, Akt, Erk1/2, JNK1/2 and transcription factors NF-κB and AP-1 were related to Cd-induced MMP-9 expression in endothelial cells. Akt, Erk1/2, and JNK1/2 functioned as upstream signals in the activation of NF-κB and AP-1, respectively. In addition, N-acetyl-L-cystein (NAC), diphenyleneiodonium chloride (DPI) and apocynin (APO) inhibited the Cd-induced activation of EGFR, Akt, Erk1/2, JNK1/2, and p38 MAPK, indicating that ROS production by NADPH oxidase is the furthest upstream signal in MMP-9 expression. At present, it states that Cd displayed marked invasiveness in ECV304 cells, which was partially abrogated by MMP-9 neutralizing antibodies. These results demonstrated that Cd induces MMP-9 expression via ROS-dependent EGFR- > Erk1/2, JNK1/2- > AP-1 and EGFR- > Akt- > NF-κB signaling pathways and, in turn

The development of a 200 kV monochromated field emission electron source

Energy Technology Data Exchange (ETDEWEB)

Mukai, Masaki, E-mail: mmukai@jeol.co.jp [JEOL Ltd., 3-1-2 Musashino, Akishima, Tokyo 196-8558 (Japan); Kim, Judy S. [University of Oxford, Department of Materials, Parks Road, Oxford, OX1 3PH (United Kingdom); Omoto, Kazuya; Sawada, Hidetaka; Kimura, Atsushi; Ikeda, Akihiro; Zhou, Jun; Kaneyama, Toshikatsu [JEOL Ltd., 3-1-2 Musashino, Akishima, Tokyo 196-8558 (Japan); Young, Neil P.; Warner, Jamie H.; Nellist, Peter D.; Kirkland, Angus I. [University of Oxford, Department of Materials, Parks Road, Oxford, OX1 3PH (United Kingdom)

2014-05-01

We report the development of a monochromator for an intermediate-voltage aberration-corrected electron microscope suitable for operation in both STEM and TEM imaging modes. The monochromator consists of two Wien filters with a variable energy selecting slit located between them and is located prior to the accelerator. The second filter cancels the energy dispersion produced by the first filter and after energy selection forms a round monochromated, achromatic probe at the specimen plane. The ultimate achievable energy resolution has been measured as 36 meV at 200 kV and 26 meV at 80 kV. High-resolution Annular Dark Field STEM images recorded using a monochromated probe resolve Si–Si spacings of 135.8 pm using energy spreads of 218 meV at 200 kV and 217 meV at 80 kV respectively. In TEM mode an improvement in non-linear spatial resolution to 64 pm due to the reduction in the effects of partial temporal coherence has been demonstrated using broad beam illumination with an energy spread of 134 meV at 200 kV. - Highlights: • Monochromator for 200 kV aberration corrected TEM and STEM was developed. • Monochromator produces monochromated and achromatic probe at specimen plane. • Ultimate energy resolution was measured to be 36 meV at 200 kV and 26 meV at 80 kV. • Atomic resolution STEM images were recorded using monochromated electron probe. • Improvements of TEM resolution were confirmed using monochromated illumination.

The development of a 200 kV monochromated field emission electron source

International Nuclear Information System (INIS)

Mukai, Masaki; Kim, Judy S.; Omoto, Kazuya; Sawada, Hidetaka; Kimura, Atsushi; Ikeda, Akihiro; Zhou, Jun; Kaneyama, Toshikatsu; Young, Neil P.; Warner, Jamie H.; Nellist, Peter D.; Kirkland, Angus I.

2014-01-01

We report the development of a monochromator for an intermediate-voltage aberration-corrected electron microscope suitable for operation in both STEM and TEM imaging modes. The monochromator consists of two Wien filters with a variable energy selecting slit located between them and is located prior to the accelerator. The second filter cancels the energy dispersion produced by the first filter and after energy selection forms a round monochromated, achromatic probe at the specimen plane. The ultimate achievable energy resolution has been measured as 36 meV at 200 kV and 26 meV at 80 kV. High-resolution Annular Dark Field STEM images recorded using a monochromated probe resolve Si–Si spacings of 135.8 pm using energy spreads of 218 meV at 200 kV and 217 meV at 80 kV respectively. In TEM mode an improvement in non-linear spatial resolution to 64 pm due to the reduction in the effects of partial temporal coherence has been demonstrated using broad beam illumination with an energy spread of 134 meV at 200 kV. - Highlights: • Monochromator for 200 kV aberration corrected TEM and STEM was developed. • Monochromator produces monochromated and achromatic probe at specimen plane. • Ultimate energy resolution was measured to be 36 meV at 200 kV and 26 meV at 80 kV. • Atomic resolution STEM images were recorded using monochromated electron probe. • Improvements of TEM resolution were confirmed using monochromated illumination

Automated Plasma Spray (APS) process feasibility study: Plasma spray process development and evaluation

Science.gov (United States)

Fetheroff, C. W.; Derkacs, T.; Matay, I. M.

1979-01-01

An automated plasma spray (APS) process was developed to apply two layer (NiCrAlY and ZrO2-12Y2O3) thermal-barrier coatings to aircraft gas turbine engine blade airfoils. The APS process hardware consists of four subsystems: a mechanical blade positioner incorporating two interlaced six-degree-of-freedom assemblies; a noncoherent optical metrology subsystem; a microprocessor-based adaptive system controller; and commercial plasma spray equipment. Over fifty JT9D first stage turbine blades specimens were coated with the APS process in preliminary checkout and evaluation studies. The best of the preliminary specimens achieved an overall coating thickness uniformity of + or - 53 micrometers, much better than is achievable manually. Factors limiting this performance were identified and process modifications were initiated accordingly. Comparative evaluations of coating thickness uniformity for manually sprayed and APS coated specimens were initiated. One of the preliminary evaluation specimens was subjected to a torch test and metallographic evaluation.

Touch and step potential analysis at 23.9kV to 4.16kV & 13.8kV to 4.16kV distribution substations with pad-mounted transformers, floating grounds, and other exposed ungrounded metal bodies using WinIGS

Science.gov (United States)

Guzman, David G.

An electrical substation is composed of various subsystems that allow for the effective and safe operation of the power grid. One of the subsystems integrating a conventional substation is defined as the ground grid system. This system allows for the effective operation of the power grid and all the electrical equipment connected to it by providing a ground potential reference, commonly known as the system ground. In addition, the ground grid system provides safety to the workers and the public transiting inside or living nearby a substation by reducing the step and touch potential (or voltage) levels present during a system fault. In today's utility industry practices there is an increasing trend for using pad-mounted electrical equipment for substation applications in an effort to construct new or upgrade existing electrical facilities inside limited property spaces. This thesis work presents an analysis for the effects of touch and step voltages at existing distribution substations where 23.9kV to 4.16kV & 13.8kV to 4.16kV pad-mounted transformers and other pad-mounted switchgear was installed to replace the traditional station class equipment. Moreover, this study will expose modeling techniques employed to define and determine the effects of floating grounds and other exposed metal bodies inside or surrounding these substations using WinIGS; this is in an effort to determine any risks of electric shock associated with this type of installations. The results presented in this work are intended to verify the requirements for the ground grid analysis and design for 4.16kV distribution substations with pad-mounted equipment in order to prevent dangerous step and touch voltage levels appearing at these sites during system faults; and ultimately prevent exposing individuals to the risk of an electric shock.

Optimization of mass flow rate in RGTT200K coolant purification for Carbon Monoxide conversion process

International Nuclear Information System (INIS)

Sumijanto; Sriyono

2016-01-01

Carbon monoxide is a species that is difficult to be separated from the reactor coolant helium because it has a relatively small molecular size. So it needs a process of conversion from carbon monoxide to carbondioxide. The rate of conversion of carbon monoxide in the purification system is influenced by several parameters including concentration, temperature and mass flow rate. In this research, optimization of the mass flow rate in coolant purification of RGTT200K for carbon monoxide conversion process was done. Optimization is carried out by using software Super Pro Designer. The rate of reduction of reactant species, the growth rate between the species and the species products in the conversion reactions equilibrium were analyzed to derive the mass flow rate optimization of purification for carbon monoxide conversion process. The purpose of this study is to find the mass flow rate of purification for the preparation of the basic design of the RGTT200K coolant helium purification system. The analysis showed that the helium mass flow rate of 0.6 kg/second resulted in an un optimal conversion process. The optimal conversion process was reached at a mass flow rate of 1.2 kg/second. A flow rate of 3.6 kg/second – 12 kg/second resulted in an ineffective process. For supporting the basic design of the RGTT200K helium purification system, the mass flow rate for carbon monoxide conversion process is suggested to be 1.2 kg/second. (author)

Infrared Astronomy Professional Development for K-12 Educators: WISE Telescope

Science.gov (United States)

Borders, Kareen; Mendez, B. M.

2010-01-01

K-12 educators need effective and relevant astronomy professional development. WISE Telescope (Wide-Field Infrared Survey Explorer) and Spitzer Space Telescope Education programs provided an immersive teacher professional development workshop at Arecibo Observatory in Puerto Rico during the summer of 2009. As many common misconceptions involve scale and distance, teachers worked with Moon/Earth scale, solar system scale, and distance of objects in the universe. Teachers built and used basic telescopes, learned about the history of telescopes, explored ground and satellite based telescopes, and explored and worked on models of WISE Telescope. An in-depth explanation of WISE and Spitzer telescopes gave participants background knowledge for infrared astronomy observations. We taught the electromagnetic spectrum through interactive stations. The stations included an overview via lecture and power point, the use of ultraviolet beads to determine ultraviolet exposure, the study of WISE lenticulars and diagramming of infrared data, listening to light by using speakers hooked up to photoreceptor cells, looking at visible light through diffraction glasses and diagramming the data, protocols for using astronomy based research in the classroom, and infrared thermometers to compare environmental conditions around the observatory. An overview of LIDAR physics was followed up by a simulated LIDAR mapping of the topography of Mars. We will outline specific steps for K-12 infrared astronomy professional development, provide data demonstrating the impact of the above professional development on educator understanding and classroom use, and detail future plans for additional K-12 professional development. Funding was provided by WISE Telescope, Spitzer Space Telescope, Starbucks, Arecibo Observatory, the American Institute of Aeronautics and Astronautics, and the Washington Space Grant Consortium.

Processing of indium (III solutions via ion exchange with Lewatit K-2621 resin

Directory of Open Access Journals (Sweden)

López Díaz-Pavón, Adrián

2014-06-01

Full Text Available The processing of indium(III-hydrochloric acid solutions by the cationic ion exchange Lewatit K-2621 resin has been investigated. The influence of several variables such as the hydrochloric acid and metal concentrations in the aqueous solution and the variation of the amount of resin added has been studied. Moreover, a kinetic study performed in the uptake of indium(III by Lewatit K-2621, shows that either the film-diffusion and the particle-diffusion models fit the ion exchange process onto the resin, depending upon the initial metal concentration in the aqueous solution. The loaded resin could be eluted by HCl solutions at 20 °C.Se ha investigado el tratamiento de disoluciones de ácido clorhídrico conteniendo indio(III mediante la resina de cambio catiónico Lewatit K-2621. Las variables ensayadas han sido las concentraciones de ácido y de metal en la disolución acuosa y la cantidad de resina empleada en el tratamiento de dichas disoluciones. Asimismo, se ha llevado a cabo un estudio cinético del proceso de intercambio catiónico entre el indio(III y la resina Lewatit K-2621. Este estudio muestra que el proceso de intercambio responde a un mecanismo de difusión en la disolución o en la partícula de resina dependiendo de la concentración inicial del metal en el medio acuoso. El metal cargado en la resina puede ser eluido con disoluciones de ácido clorhídrico a 20 °C.

Almost there: Sector 7-8 being cooled to 4.5K!

CERN Multimedia

2007-01-01

The cool down of the 3km long LHC sector 7-8 between point 7 (Ferney-Voltaire) and point 8 (Prévessin) has entered a new phase. The cryostats with the superconducting magnets, which had been kept around 20 K (-253°C) for the past week, are gradually being cooled down to 4.5 K (-268°C) and filled with liquid helium. This new phase in the cooling process was successfully started on Monday 5 March and is progressing after a tuning session that lasted most of the day. To get down to 1.9K (-271°C), a new component of the cryogenics plant, the 1.8K units, will be turned on. In the meantime the teams who will carry out the magnet powering are busy validating the test procedures and running the interlock tests to ensure safe operation of the equipment. They are ready to start the powering as soon as the cryo team reaches stable conditions at 1.9 K. Follow the cool down on the Hardware Commissioning Coordination website.

Developing an Engineering Design Process Assessment using Mixed Methods.

Science.gov (United States)

Wind, Stefanie A; Alemdar, Meltem; Lingle, Jeremy A; Gale, Jessica D; Moore, Roxanne A

Recent reforms in science education worldwide include an emphasis on engineering design as a key component of student proficiency in the Science, Technology, Engineering, and Mathematics disciplines. However, relatively little attention has been directed to the development of psychometrically sound assessments for engineering. This study demonstrates the use of mixed methods to guide the development and revision of K-12 Engineering Design Process (EDP) assessment items. Using results from a middle-school EDP assessment, this study illustrates the combination of quantitative and qualitative techniques to inform item development and revisions. Overall conclusions suggest that the combination of quantitative and qualitative evidence provides an in-depth picture of item quality that can be used to inform the revision and development of EDP assessment items. Researchers and practitioners can use the methods illustrated here to gather validity evidence to support the interpretation and use of new and existing assessments.

Constitutive Behavior and Processing Map of T2 Pure Copper Deformed from 293 to 1073 K

Science.gov (United States)

Liu, Ying; Xiong, Wei; Yang, Qing; Zeng, Ji-Wei; Zhu, Wen; Sunkulp, Goel

2018-02-01

The deformation behavior of T2 pure copper compressed from 293 to 1073 K with strain rates from 0.01 to 10 s-1 was investigated. The constitutive equations were established by the Arrhenius constitutive model, which can be expressed as a piecewise function of temperature with two sections, in the ranges 293-723 K and 723-1073 K. The processing maps were established according to the dynamic material model for strains of 0.2, 0.4, 0.6, and 0.8, and the optimal processing parameters of T2 copper were determined accordingly. In order to obtain a better understanding of the deformation behavior, the microstructures of the compressed samples were studied by electron back-scattered diffraction. The grains tend to be more refined with decreases in temperature and increases in strain rate.

Hibiscus sabdariffa Leaf Extract Inhibits Human Prostate Cancer Cell Invasion via Down-Regulation of Akt/NF-kB/MMP-9 Pathway.

Science.gov (United States)

Chiu, Chun-Tang; Chen, Jing-Hsien; Chou, Fen-Pi; Lin, Hui-Hsuan

2015-06-24

Hibiscus sabdariffa leaf has been previously shown to possess hypoglycemic, hypolipidemic, and antioxidant effects, and induce tumor cell apoptosis. However, the molecular mechanisms involved in the anticancer activity of H. sabdariffa leaf extract (HLE) are poorly understood. The object of the study was to examine the anti-invasive potential of HLE. First, HLE was demonstrated to be rich in polyphenols. The results of wound-healing assay and in vitro transwell assay revealed that HLE dose-dependently inhibited the migration and invasion of human prostate cancer LNCaP (lymph node carcinoma of the prostate) cells under non-cytotoxic concentrations. Our results further showed that HLE exerted an inhibitory effect on the activity and expressions of matrix metalloproteinase-9 (MMP-9). The HLE-inhibited MMP-9 expression appeared to be a consequence of nuclear factor-kappaB (NF-κB) inactivation because its DNA-binding activity was suppressed by HLE. Molecular data showed all these influences of HLE might be mediated via inhibition of protein kinase B (PKB, also known as Akt)/NF-kB/MMP-9 cascade pathway, as demonstrated by the transfection of Akt1 overexpression vector. Finally, the inhibitory effect of HLE was proven by its inhibition on the growth of LNCaP cells and the expressions of metastasis-related molecular proteins in vivo. These findings suggested that the inhibition of MMP-9 expression by HLE may act through the suppression of the Akt/NF-kB signaling pathway, which in turn led to the reduced invasiveness of the cancer cells.

MET-2-Dependent H3K9 Methylation Suppresses Transgenerational Small RNA Inheritance.

Science.gov (United States)

Lev, Itamar; Seroussi, Uri; Gingold, Hila; Bril, Roberta; Anava, Sarit; Rechavi, Oded

2017-04-24

In C. elegans, alterations to chromatin produce transgenerational effects, such as inherited increase in lifespan and gradual loss of fertility. Inheritance of histone modifications can be induced by double-stranded RNA-derived heritable small RNAs. Here, we show that the mortal germline phenotype, which is typical of met-2 mutants, defective in H3K9 methylation, depends on HRDE-1, an argonaute that carries small RNAs across generations, and is accompanied by accumulated transgenerational misexpression of heritable small RNAs. We discovered that MET-2 inhibits small RNA inheritance, and, as a consequence, induction of RNAi in met-2 mutants leads to permanent RNAi responses that do not terminate even after more than 30 generations. We found that potentiation of heritable RNAi in met-2 animals results from global hyperactivation of the small RNA inheritance machinery. Thus, changes in histone modifications can give rise to drastic transgenerational epigenetic effects, by controlling the overall potency of small RNA inheritance. Copyright © 2017 Elsevier Ltd. All rights reserved.

Search for $\\mathit{\\Lambda_{b}\\to K^{-}\\mu^{+}}$ at LHCb

CERN Document Server

AUTHOR|(INSPIRE)INSPIRE-00264595; Hansmann-Menzemer, Stephanie

In this thesis the search for the decays $\\mathit{\\Lambda_{b}\\to K^{-}\\mu^{+}}$ and $\\mathit{\\Xi_{b}^{\\rm 0}\\to K^{-}\\mu^{+}}$ is presented using the Run~I data of the LHCb experiment. Both decays offer an ansatz to the description of the matter-antimatter asymmetry in our universe and is based on the Sakharov conditions that predict the existence of processes violating the baryon and lepton number conservation. Especially the measured baryon-to-photon ratio $\\eta\\approx6\\cdot10^{-10}$ allows to estimate the probability of such processes in this order of magnitude. In the underlying data set including about $50$ billion recorded $\\mathit{\\Lambda_{b}}$ decays no signal events are observed and an upper limit on the combined branching fraction is determined at a confidence level of $90\\,\\%$: $$ \\left[{\\mathcal B}(\\mathit{\\Lambda_{b}\\to K^{-}\\mu^{+}})+{\\mathcal B}(\\mathit{\\Lambda_{b}\\to K^{+}\\mu^{-}})\\right] \\times\\frac{3.1\\cdot10^{-6}}{{\\mathcal B}(\\mathit{\\Lambda_{b}\\to p K^{-}})} < 1.95\\cdot10^{-9}~. $...

UNION-K 2.0 User's Manual

International Nuclear Information System (INIS)

Yoo, Jae Woon; Jang, Jin Wook; Kim, Yeong Il

2008-12-01

The unified Input/Output processor UNION-K(UNified Input/Output Processor for Neutronics Analysis by K-CORE) has been developed to provide the automatic input file generation and output processing for the fast reactor core analysis. UNION-K includes the material database for reactor analysis and produces the input files for effective cross section generation, core analysis, and reactivity coefficient calculations. UNION-K can execute corresponding core analysis module and edit core performance parameters from the outputs. UNION-K also provides the graphical interface to check the geometrical arrangement of core and fuel sub-assembly and produces the picture files that can be used for reporting. This report has been prepared for instruction of using UNION-K Ver. 2.0 with examples

Thermodynamic properties of vitamin B_9

International Nuclear Information System (INIS)

Knyazev, A.V.; Emel’yanenko, V.N.; Shipilova, A.S.; Lelet, M.I.; Gusarova, E.V.; Knyazeva, S.S.; Verevkin, S.P.

2016-01-01

Highlights: • Temperature dependence of heat capacity of vitamin B_9 has been measured by precision adiabatic vacuum calorimetry. • The thermodynamic functions of the vitamin B_9 have been determined for the range from T → 0 to 333 K. • The character of heterodynamics of structure was detected. • Enthalpy of combustion of the vitamin B_9 was measured using high-precision combustion calorimeter. - Abstract: In the present work temperature dependence of heat capacity of vitamin B_9 (folic acid dihydrate) has been measured for the first time in the range from (6 to 333) K by precision adiabatic vacuum calorimetry. Based on the experimental values, the thermodynamic functions of the vitamin B_9, namely, the heat capacity, enthalpy H°(T) − H°(0), entropy S°(T) − S°(0) and Gibbs function G°(T) − H°(0) have been determined for the range from T → (0 to 333) K. The value of the fractal dimension D in the function of multifractal generalization of Debye’s theory of the heat capacity of solids was estimated and the character of heterodynamics of structure was detected. Enthalpy of combustion (−8942.8 ± 7.5) kJ·mol"−"1 of the vitamin B_9 was measured for the first time using a high-precision combustion calorimeter. The standard molar enthalpy of formation in the crystalline state (−1821.0 ± 7.9) kJ·mol"−"1 of B_9 at 298.15 K was derived from the combustion experiments. Using a combination of the adiabatic and combustion calorimetry results, the thermodynamic functions of formation of the folic acid dihydrate at T = 298.15 K and p = 0.1 MPa have been calculated. The low-temperature X-ray diffraction was used for the determination of coefficients of thermal expansion.

Development of a 15 K hydrogen-based sorption cooler

NARCIS (Netherlands)

Burger, Johannes Faas; Holland, Herman J.; Meijer, R.J.; Linder, M.; ter Brake, Hermanus J.M.

2010-01-01

At the University of Twente, a 15 K hydrogen-based sorption cooler is under development, which has no moving parts and, therefore, is essentially vibration-free. Moreover, it has the potential of a very long life. Although the cooler may operate standalone, it is designed to precool a helium-based

Task 9: deployment of photovoltaic technologies: co-operation with developing countries. Sources of financing for PV-based rural electrification in developing countries

Energy Technology Data Exchange (ETDEWEB)

Parker, W. [Institute for Sustainable Power, Highlands Ranch, CO (United States); Syngellakis, K. [IT Power Ltd, The Manor house, Chineham (United Kingdom); Shanker, A. [Innovation Energie Developpement, IED, Francheville (France)

2004-05-15

This report for the International Energy Agency (IEA) made by Task 9 of the Photovoltaic Power Systems (PVPS) programme takes a look at how PV-based rural electrification in developing countries can be financed. The objective of Task 9 is to increase the overall rate of successful deployment of PV systems in developing countries through increased co-operation and information exchange. This document provides an introduction to PV project financing, including funding sources available, strategies and planning needed to secure the necessary financial resources for the deployment of PV technologies in developing and transitional economies. Topics discussed include risk analysis and the barriers to financing, sources of financing, considerations and variables that influence financing decisions and the process for securing financing. Various forms of international and national financing are looked at, as are the factors influencing financing decisions.

AtCHX13 is a plasma membrane K(+) transporter

Science.gov (United States)

Potassium (K+) homeostasis is essential for diverse cellular processes, although how various cation transporters collaborate to maintain a suitable K(+) required for growth and development is poorly understood. The Arabidopsis ("Arabidopsis thaliana") genome contains numerous cation:proton antiporte...

AtCHX13 is a plasma membrane K+ transporter

Science.gov (United States)

Potassium (K+) homeostasis is essential for diverse cellular processes, although how various cation transporters collaborate to maintain a suitable K+ required for growth and development is poorly understood. The Arabidopsis (Arabidopsis thaliana) genome contains numerous cation:proton antiporters (...

9 CFR 416.12 - Development of Sanitation SOP's.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Development of Sanitation SOP's. 416... ACT SANITATION § 416.12 Development of Sanitation SOP's. (a) The Sanitation SOP's shall describe all... direct contamination or adulteration of product(s). (b) The Sanitation SOP's shall be signed and dated by...

K-12 Professional Development at the Harvard Forest LTER

Science.gov (United States)

Bennett, K.

2012-12-01

As part of the Long Term Ecological Research (LTER) program, the Harvard Forest in Petersham, Massachusetts seeks to train the next generation of researchers, by involving K-12 grade students and their teachers in hands-on, field-based, ecological research in their own schoolyard and community. Students learn to collect data on important long-term ecological issues and processes. Student data are then shared on the Harvard Forest website. To prepare teachers for project protocols, teachers are given direct access to Harvard ecologists with professional development workshops and on-line resources. With the Harvard Forest Schoolyard LTER program, students can participate in three different research projects focusing on phenology, invasive insects, and vernal pools. Teachers attend the Summer Institute for Teachers to learn project content and methods. They return in fall to participate in one of three levels of data workshops to learn how to input, manage, and analyze project data. In the spring, teachers again meet with the Harvard ecologists about project protocols, and to share, through a series of teacher presentations, the ways these project themes are being integrated into class curricula. These professional development opportunities result in long term collaborative partnerships with local schools and the Harvard Forest LTER. In addition to the LTER Schoolyard Ecology Program, the Harvard Forest has supported a successful Research Experience for Teachers (RET) program for the last six years. Throughout the summer, teachers work on research projects alongside Harvard Forest and affiliated scientists, post-docs, graduate students, and REU's (Research Experience for Undergraduates). The RET program provides teachers with the opportunity to build scientific knowledge, develop an understanding of research methods, and translate their new knowledge and experiences into cutting edge classroom lessons. The past two summers I have worked with Dr. Andrew Richardson

Repeat Composition of CenH3-chromatin and H3K9me2-marked heterochromatin in Sugar Beet (Beta vulgaris)

Czech Academy of Sciences Publication Activity Database

Kowar, T.; Zakrzewski, F.; Macas, Jiří; Koblížková, Andrea; Viehoever, P.; Weisshaar, B.; Schmidt, T.

2016-01-01

Roč. 16, č. 120 (2016) ISSN 1471-2229 R&D Projects: GA ČR GBP501/12/G090 Institutional support: RVO:60077344 Keywords : Centromere * CenH3 * H3K9me2 * Heterochromatin Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.964, year: 2016

DEVELOPING INDUSTRIAL ROBOT SIMULATION MODEL TUR10-K USING “UNIVERSAL MECHANISM” SOFTWARE COMPLEX

Directory of Open Access Journals (Sweden)

Vadim Vladimirovich Chirkov

2018-02-01

Full Text Available Manipulation robots are complex spatial mechanical systems having five or six degrees of freedom, and sometimes more. For this reason, modeling manipulative robots movement, even in the kinematic formulation, is a complex mathematical task. If one moves from kinematic modeling of motion to dynamic modeling then there must be taken into account the inertial properties of the modeling object. In this case, analytical constructing of such a complex object mathematical model as a manipulation robot becomes practically impossible. Therefore, special computer-aided design systems, called CAE-systems, are used for modeling complex mechanical systems. The purpose of the paper is simulation model construction of a complex mechanical system, such as the industrial robot TUR10-K, to obtain its dynamic characteristics. Developing such models makes it possible to reduce the complexity of designing complex systems process and to obtain the necessary characteristics. Purpose. Developing the simulation model of the industrial robot TUR10-K and obtaining dynamic characteristics of the mechanism. Methodology: the article is used a computer simulation method. Results: There is obtained the simulation model of the robot and its dynamic characteristics. Practical implications: the results can be used in the mechanical systems design and various simulation models.

Instrumental development and data processing

International Nuclear Information System (INIS)

Franzen, J.

1978-01-01

A review of recent developments in mass spectrometry instrumentation is presented under the following headings: introduction (scope of mass spectrometry compared with neighbouring fields); ion sources and ionization techniques; spectrometers (instrumental developments); measuring procedures; coupling techniques; data systems; conclusions (that mass spectrometry should have a broader basis and that there would be mutual profit from a better penetration of mass spectrometry into fields of routine application). (U.K.)

Development of technology for production of reduced fat processed cheese

Directory of Open Access Journals (Sweden)

Adriana Torres Silva e Alves

2015-09-01

Full Text Available An increasing share of foods with reduced fat has been observed in the diet of the Brazilian population, a trend also seen in many other countries. In this context, our-aim was to study the manufacturing parameters and to develop a process to produce a spreadable processed cheese (requeijão cremoso with reduced fat content. In the first stage of this study, modifications were performed in the traditional manufacturing process of requeijão cremoso with regular fat content to produce a reduced fat product. During the second stage of this study, two reduced fat cheeses, with and withoutthe addition of whey protein concentrate (WPC were developed, both using JOHA S9 and JOHA PZ as emulsifying salts, resulting in four different formulations. The amounts of cream and water used in both products were calculated in order to obtain a final product with 10% fat and 33% total solids. The product which presented the best results was produced with curd obtained by direct acidification of skimmed milk heated at 68-70 ºC, using 1,3% emulsifying salt JOHA S9 in the melting process and 2% WPC 34% as a partial fat substitute, both calculated as a percentage of the amountof curd used as raw material. It was also important to add WPC 34% to the product at the first cooking step of the process (70 ºC, in order to obtain a final product withthe typical spreadable texture of the traditional requeijão cremoso.

Thermomechanical Processing of Fe-6.9Al-2Cr-0.88C Steel: Intercritical Annealing Followed by Quench Tempering

Science.gov (United States)

Farahat, Ahmed Ismail Zaky; Mohamed, Masoud Ibrahim

2015-01-01

A hot forged Fe-0.88 pct C-6.9 pct Al steel was intercritically annealed at temperatures in the range of 1173 K to 1283 K (900 °C to 1010 °C), and subsequently tempered at 623 K (350 °C) to enhance the mechanical properties by microstructure modification. Room temperature compression tests were carried out to evaluate the influence of the intercritical annealing temperature on the mechanical properties. A substructure was present in the microstructure after each intercritical annealing treatment. The substructure was absent after annealing at 1263 K (990 °C) and higher temperatures. Over-aging occurred when the annealing temperature was increased to 1283 K (1010 °C). A remarkable increase in strength and ductility was achieved after annealing at 1263 K (990 °C).

Eliciting and Developing Teachers' Conceptions of Random Processes in a Probability and Statistics Course

Science.gov (United States)

Smith, Toni M.; Hjalmarson, Margret A.

2013-01-01

The purpose of this study is to examine prospective mathematics specialists' engagement in an instructional sequence designed to elicit and develop their understandings of random processes. The study was conducted with two different sections of a probability and statistics course for K-8 teachers. Thirty-two teachers participated. Video analyses…

Anti-cooperative supramolecular polymerization: a new K2-K model applied to the self-assembly of perylene bisimide dye proceeding via well-defined hydrogen-bonded dimers.

Science.gov (United States)

Gershberg, Jana; Fennel, Franziska; Rehm, Thomas H; Lochbrunner, Stefan; Würthner, Frank

2016-03-01

A perylene bisimide dye bearing amide functionalities at the imide positions derived from amino acid l-alanine and a dialkoxy-substituted benzyl amine self-assembles into tightly bound dimers by π-π-stacking and hydrogen bonding in chloroform. In less polar or unpolar solvents like toluene and methylcyclohexane, and in their mixtures, these dimers further self-assemble into extended oligomeric aggregates in an anti-cooperative process in which even numbered aggregates are highly favoured. The stepwise transition from dimers into oligomers can not be properly described by conventional K 2 - K model, and thus a new K 2 - K aggregation model has been developed, which interpretes the present anti-cooperative supramolecular polymerization more appropriately. The newly developed K 2 - K model will be useful to describe self-assembly processes of a plethora of other π-conjugated molecules that are characterized by a favored dimer species.

Measurement of the decays B --> phi K and B --> K*

International Nuclear Information System (INIS)

Aubert, B.

2001-01-01

We have observed the decays B → phi K and phi K* in a sample of over 45 million B mesons collected with the BABAR detector at the PEP-II collider. The measured branching fractions are BF(B + → phi K + ) = (7.7 -1.4 +1.6 +/- 0.8) x 10 -6 , BF(B 0 → phi K 0 ) =(8.1 -2.5 +3.1 +/- 0.8) x 10 -6 , BF(B + → phi K* + ) = (9.7 -3.4 +4.2 +/- 1.7) x 10 -6 , and BF(B 0 → phi K* 0 ) = (8.6 -2.4 +2.8 +/- 1.1) x 10 -6 . We also report the upper limit BF(B + → phi pi + ) -6 (90 percent CL)

A thermally switched 9 kA superconducting rectifier fluxpump

NARCIS (Netherlands)

ten Kate, Herman H.J.; Bunk, Paul B.; Steffens, Harry A.; van de Klundert, Louis J.M.

1981-01-01

The feasibility of superconducting rectifier-fluxpumps has to be demonstrated at current levels of 10 - 100 kA, where is asked for in the superconducting devices now being planned. An intensive program has been started at the low temperature division of the University of Twente to construct such

Manjeet K Sangha

Indian Academy of Sciences (India)

Home; Journals; Resonance – Journal of Science Education. Manjeet K Sangha. Articles written in Resonance – Journal of Science Education. Volume 9 Issue 8 August 2004 pp 35-45 General Article. Detergents – Zeolites and Enzymes Excel Cleaning Power · B S Sekhon Manjeet K Sangha · More Details Fulltext PDF ...

submitter Performance Test of an 8 kA @ 10-T 4.2-K ReBCO-CORC Cable

CERN Document Server

Mulder, Tim; Mentink, Matthias; van der Laan, Danko; Dhalle, Marc; ten Kate, Herman

2016-01-01

CERN is developing high-current ReBCO conductor on round core (CORC) cables for application in future detector and accelerator magnets. A characterization test on a ReBCO-CORC cable sample and its joints is performed in the 10-T FRESCA cable test facility at CERN. The sample is taken from the first 12-m-long CORC production. Key is the characterization of the field- and temperature-dependent critical currents of the CORC cable at 1.9 K and 4.2 K. Secondary objectives include evaluating the response of the CORC cable to quenches and the performance of cylindrical low resistive cable terminals especially designed and manufactured for use on CORC cables. The 7.6-mm CORC cable features 8 kA at 4.2 K and 10 T, and the joint terminals show a 25 ± 5 - nΩ resistance for 20-cm length.

Using Action Research to Engage K-6 Teachers in Nature of Science Inquiry as Professional Development

Science.gov (United States)

Cullen, Theresa A.; Akerson, Valarie L.; Hanson, Deborah L.

2010-12-01

Teachers are required to work with data on a daily basis to assess the effectiveness of their teaching strategies, but may not approach it as research. This paper presents a reflective discussion of how and when a professional development team used an action research project to help 12 K-6 teachers explore the effectiveness of reform based Nature of Science (NOS) teaching strategies in their classrooms. The team encouraged community development and provided “just in time” supports to scaffold the steps of the action research process for teachers. The discussion includes concerns they addressed and issues related to management and support of the professional development model. Evaluation results are shared to suggest how this approach can be improved in the future.

A Major Facilitator Superfamily protein encoded by TcMucK gene is not required for cuticle pigmentation, growth and development in Tribolium castaneum.

Science.gov (United States)

Mun, Seulgi; Noh, Mi Young; Osanai-Futahashi, Mizuko; Muthukrishnan, Subbaratnam; Kramer, Karl J; Arakane, Yasuyuki

2014-06-01

Insect cuticle pigmentation and sclerotization (tanning) are vital physiological processes for insect growth, development and survival. We have previously identified several colorless precursor molecules as well as enzymes involved in their biosynthesis and processing to yield the mature intensely colored body cuticle pigments. A recent study indicated that the Bombyx mori (silkmoth) gene, BmMucK, which encodes a protein orthologous to a Culex pipiens quiquefasciatus (Southern house mosquito) cis,cis, muconate transporter, is a member of the "Major Facilitator Superfamily" (MFS) of transporter proteins and is associated with the appearance of pigmented body segments of naturally occurring body color mutants of B. mori. While RNA interference of the BmMucK gene failed to result in any observable phenotype, RNAi using a dsRNA for an orthologous gene from the red flour beetle, Tribolium castaneum, was reported to result in molting defects and darkening of the cuticle and some body parts, leading to the suggestion that orthologs of MucK genes may differ in their functions among insects. To verify the role and essentiality of the ortholog of this gene in development and body pigmentation function in T. castaneum we obtained cDNAs for the orthologous gene (TcMucK) from RNA isolated from the GA-1 wild-type strain of T. castaneum. The sequence of a 1524 nucleotides-long cDNA for TcMucK which encodes the putatively full-length protein, was assembled from two overlapping RT-PCR fragments and the expression profile of this gene during development was analyzed by real-time PCR. This cDNA encodes a 55.8 kDa protein consisting of 507 amino acid residues and includes 11 putative transmembrane segments. Transcripts of TcMucK were detected throughout all of the developmental stages analyzed. The function of this gene was explored by injection of two different double-stranded RNAs targeting different regions of the TcMucK gene (dsTcMucKs) into young larvae to down

Prostaglandins in the kidney: developments since Y2K.

Science.gov (United States)

Nasrallah, Rania; Clark, Jordan; Hébert, Richard L

2007-10-01

There are five major PGs (prostaglandins/prostanoids) produced from arachidonic acid via the COX (cyclo-oxygenase) pathway: PGE(2), PGI(2) (prostacyclin), PGD(2), PGF(2alpha) and TXA(2) (thromboxane A(2)). They exert many biological effects through specific G-protein-coupled membrane receptors, namely EP (PGE(2) receptor), IP (PGI(2) receptor), DP (PGD(2) receptor), FP (PGF(2alpha) receptor) and TP (TXA(2) receptor) respectively. PGs are implicated in physiological and pathological processes in all major organ systems, including cardiovascular function, gastrointestinal responses, reproductive processes, renal effects etc. This review highlights recent insights into the role of each prostanoid in regulating various aspects of renal function, including haemodynamics, renin secretion, growth responses, tubular transport processes and cell fate. A thorough review of the literature since Y2K (year 2000) is provided, with a general overview of PGs and their synthesis enzymes, and then specific considerations of each PG/prostanoid receptor system in the kidney.

Dalitz plot analysis of eta(c) -> K+K-eta and eta(c) -> K+K-pi(0) in two-photon interactions

NARCIS (Netherlands)

Lees, J. P.; Poireau, V.; Tisserand, V.; Grauges, E.; Palano, A.; Eigen, G.; Stugu, B.; Brown, D. N.; Kerth, L. T.; Kolomensky, Yu. G.; Lynch, G.; Schroeder, T.; Hearty, C.; Mattison, T. S.; McKenna, J. A.; So, R. Y.; Khan, A.; Blinov, V. E.; Buzykaev, A. R.; Druzhinin, V. P.; Golubev, V. B.; Kravchenko, E. A.; Onuchin, A. P.; Serednyakov, S. I.; Skovpen, Yu. I.; Solodov, E. P.; Todyshev, K. Yu.; Lankford, A. J.; Mandelkern, M.; Dey, B.; Gary, J. W.; Long, O.; Campagnari, C.; Sevilla, M. Franco; Hong, T. M.; Kovalskyi, D.; Richman, J. D.; West, C. A.; Eisner, A. M.; Lockman, W. S.; Vazquez, W. Panduro; Schumm, B. A.; Seiden, A.; Chao, D. S.; Echenard, B.; Flood, K. T.; Hitlin, D. G.; Miyashita, T. S.; Ongmongkolkul, P.; Andreassen, R.; Huard, Z.; Meadows, B. T.; Pushpawela, B. G.; Sokoloff, M. D.; Sun, L.; Bloom, P. C.; Ford, W. T.; Gaz, A.; Smith, J. G.; Wagner, S. R.; Ayad, R.; Toki, W. H.; Spaan, B.; Bernard, D.; Verderi, M.; Playfer, S.; Bettoni, D.; Bozzi, C.; Calabrese, R.; Cibinetto, G.; Fioravanti, E.; Garzia, I.; Luppi, E.; Piemontese, L.; Santoro, V.; Calcaterra, A.; de Sangro, R.; Finocchiaro, G.; Martellotti, S.; Patteri, P.; Peruzzi, I. M.; Piccolo, M.; Rama, M.; Zallo, A.; Contri, R.; Lo Vetere, M.; Monge, M. R.; Passaggio, S.; Patrignani, C.; Robutti, E.; Bhuyan, B.; Prasad, V.; Morii, M.; Adametz, A.; Uwer, U.; Lacker, H. M.; Dauncey, P. D.; Mallik, U.; Cochran, J.; Prell, S.; Ahmed, H.; Gritsan, A. V.; Arnaud, N.; Davier, M.; Derkach, D.; Grosdidier, G.; Le Diberder, F.; Lutz, A. M.; Malaescu, B.; Roudeau, P.; Stocchi, A.; Wormser, G.; Lange, D. J.; Wright, D. M.; Coleman, J. P.; Fry, J. R.; Gabathuler, E.; Hutchcroft, D. E.; Payne, D. J.; Touramanis, C.; Bevan, A. J.; Di Lodovico, F.; Sacco, R.; Cowan, G.; Bougher, J.; Brown, D. N.; Davis, C. L.; Denig, A. G.; Fritsch, M.; Gradl, W.; Griessinger, K.; Hafner, A.; Prencipe, E.; Schubert, K. R.; Barlow, R. J.; Lafferty, G. D.; Cenci, R.; Hamilton, B.; Jawahery, A.; Roberts, D. A.; Cowan, R.; Sciolla, G.; Cheaib, R.; Patel, P. M.; Robertson, S. H.; Neri, N.; Palombo, F.; Cremaldi, L.; Godang, R.; Sonnek, P.; Summers, D. J.; Simard, M.; Taras, P.; De Nardo, G.; Onorato, G.; Sciacca, C.; Martinelli, M.; Raven, G.; Jessop, C. P.; LoSecco, J. M.; Honscheid, K.; Kass, R.; Feltresi, E.; Margoni, M.; Morandin, M.; Posocco, M.; Rotondo, M.; Simi, G.; Simonetto, F.; Stroili, R.; Akar, S.; Ben-Haim, E.; Bomben, M.; Bonneaud, G. R.; Briand, H.; Calderini, G.; Chauveau, J.; Leruste, Ph.; Marchiori, G.; Ocariz, J.; Biasini, M.; Manoni, E.; Pacetti, S.; Rossi, A.; Angelini, C.; Batignani, G.; Bettarini, S.; Carpinelli, M.; Casarosa, G.; Cervelli, A.; Chrzaszcz, M.; Forti, F.; Giorgi, M. A.; Lusiani, A.; Oberhof, B.; Paoloni, E.; Perez, A.; Rizzo, G.; Walsh, J. J.; Pegna, D. Lopes; Olsen, J.; Smith, A. J. S.; Faccini, R.; Ferrarotto, F.; Ferroni, F.; Gaspero, M.; Li Gioi, L.; Piredda, G.; Buenger, C.; Dittrich, S.; Gruenberg, O.; Hess, M.; Leddig, T.; Voss, C.; Waldi, R.; Adye, T.; Olaiya, E. O.; Wilson, F. F.; Emery, S.; Vasseur, G.; Anulli, F.; Aston, D.; Bard, D. J.; Cartaro, C.; Convery, M. R.; Dorfan, J.; Dubois-Felsmann, G. P.; Dunwoodie, W.; Ebert, M.; Field, R. C.; Fulsom, B. G.; Graham, M. T.; Hast, C.; Innes, W. R.; Kim, P.; Leith, D. W. G. S.; Lewis, P.; Lindemann, D.; Luitz, S.; Luth, V.; Lynch, H. L.; MacFarlane, D. B.; Muller, D. R.; Neal, H.; Perl, M.; Pulliam, T.; Ratcliff, B. N.; Roodman, A.; Salnikov, A. A.; Schindler, R. H.; Snyder, A.; Su, D.; Sullivan, M. K.; Va'vra, J.; Wisniewski, W. J.; Wulsin, H. W.; Purohit, M. V.; White, R. M.; Wilson, J. R.; Randle-Conde, A.; Sekula, S. J.; Bellis, M.; Burchat, P. R.; Puccio, E. M. T.; Alam, M. S.; Ernst, J. A.; Gorodeisky, R.; Guttman, N.; Peimer, D. R.; Soffer, A.; Spanier, S. M.; Ritchie, J. L.; Ruland, A. M.; Schwitters, R. F.; Wray, B. C.; Izen, J. M.; Lou, X. C.; Bianchi, F.; De Mori, F.; Filippi, A.; Gamba, D.; Lanceri, L.; Vitale, L.; Martinez-Vidal, F.; Oyanguren, A.; Villanueva-Perez, P.; Albert, J.; Banerjee, Sw.; Beaulieu, A.; Bernlochner, F. U.; Choi, H. H. F.; Kowalewski, R.; Lewczuk, M. J.; Lueck, T.; Nugent, I. M.; Roney, J. M.; Sobie, R. J.; Tasneem, N.; Gershon, T. J.; Harrison, P. F.; Latham, T. E.; Band, H. R.; Dasu, S.; Pan, Y.; Prepost, R.

2014-01-01

We study the processes γγ→K+K−η and γγ→K+K−π0 using a data sample of 519  fb−1 recorded with the BABAR detector operating at the SLAC PEP-II asymmetric-energy e+e− collider at center-of-mass energies at and near the Υ(nS) (n=2,3,4) resonances. We observe ηc→K+K−π0 and ηc→K+K−η decays, measure their

Measurement of the decays B--> phiK and B--> phiK*.

Science.gov (United States)

Aubert, B; Boutigny, D; Gaillard, J M; Hicheur, A; Karyotakis, Y; Lees, J P; Robbe, P; Tisserand, V; Palano, A; Chen, G P; Chen, J C; Qi, N D; Rong, G; Wang, P; Zhu, Y S; Eigen, G; Reinertsen, P L; Stugu, B; Abbott, B; Abrams, G S; Borgland, A W; Breon, A B; Brown, D N; Button-Shafer, J; Cahn, R N; Clark, A R; Fan, Q; Gill, M S; Gowdy, S J; Gritsan, A; Groysman, Y; Jacobsen, R G; Kadel, R W; Kadyk, J; Kerth, L T; Kluth, S; Kolomensky, Y G; Kral, J F; LeClerc, C; Levi, M E; Liu, T; Lynch, G; Meyer, A B; Momayezi, M; Oddone, P J; Perazzo, A; Pripstein, M; Roe, N A; Romosan, A; Ronan, M T; Shelkov, V G; Telnov, A V; Wenzel, W A; Bright-Thomas, P G; Harrison, T J; Hawkes, C M; Kirk, A; Knowles, D J; O'Neale, S W; Penny, R C; Watson, A T; Watson, N K; Deppermann, T; Koch, H; Krug, J; Kunze, M; Lewandowski, B; Peters, K; Schmuecker, H; Steinke, M; Andress, J C; Barlow, N R; Bhimji, W; Chevalier, N; Clark, P J; Cottingham, W N; De Groot, N; Dyce, N; Foster, B; Mass, A; McFall, J D; Wallom, D; Wilson, F F; Abe, K; Hearty, C; Mattison, T S; McKenna, J A; Thiessen, D; Camanzi, B; Jolly, S; McKemey, A K; Tinslay, J; Blinov, V E; Bukin, A D; Bukin, D A; Buzykaev, A R; Dubrovin, M S; Golubev, V B; Ivanchenko, V N; Korol, A A; Kravchenko, E A; Onuchin, A P; Salnikov, A A; Serednyakov, S I; Skovpen, Y I; Telnov, V I; Yushkov, A N; Lankford, A J; Mandelkern, M; McMahon, S; Stoker, D P; Ahsan, A; Arisaka, K; Buchanan, C; Chun, S; Branson, J G; MacFarlane, D B; Prell, S; Rahatlou, S; Raven, G; Sharma, V; Campagnari, C; Dahmes, B; Hart, P A; Kuznetsova, N; Levy, S L; Long, O; Lu, A; Richman, J D; Verkerke, W; Witherell, M; Yellin, S; Beringer, J; Dorfan, D E; Eisner, A M; Frey, A; Grillo, A A; Grothe, M; Heusch, C A; Johnson, R P; Kroeger, W; Lockman, W S; Pulliam, T; Sadrozinski, H; Schalk, T; Schmitz, R E; Schumm, B A; Seiden, A; Turri, M; Walkowiak, W; Williams, D C; Wilson, M G; Chen, E; Dubois-Felsmann, G P; Dvoretskii, A; Hitlin, D G; Metzler, S; Oyang, J; Porter, F C; Ryd, A; Samuel, A; Weaver, M; Yang, S; Zhu, R Y; Devmal, S; Geld, T L; Jayatilleke, S; Mancinelli, G; Meadows, B T; Sokoloff, M D; Bloom, P; Fahey, S; Ford, W T; Gaede, F; Johnson, D R; Michael, A K; Nauenberg, U; Olivas, A; Park, H; Rankin, P; Roy, J; Sen, S; Smith, J G; van Hoek, W C; Wagner, D L; Blouw, J; Harton, J L; Krishnamurthy, M; Soffer, A; Toki, W H; Wilson, R J; Zhang, J; Brandt, T; Brose, J; Colberg, T; Dahlinger, G; Dickopp, M; Dubitzky, R S; Maly, E; Müller-Pfefferkorn, R; Otto, S; Schubert, K R; Schwierz, R; Spaan, B; Wilden, L; Behr, L; Bernard, D; Bonneaud, G R; Brochard, F; Cohen-Tanugi, J; Ferrag, S; Roussot, E; T'Jampens, S; Thiebaux, C; Vasileiadis, G; Verderi, M; Anjomshoaa, A; Bernet, R; Di Lodovico, F; Khan, A; Muheim, F; Playfer, S; Swain, J E; Falbo, M; Bozzi, C; Dittongo, S; Folegani, M; Piemontese, L; Treadwell, E; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; de Sangro, R; Falciai, D; Finocchiaro, G; Patteri, P; Peruzzi, I M; Piccolo, M; Xie, Y; Zallo, A; Bagnasco, S; Buzzo, A; Contri, R; Crosetti, G; Fabbricatore, P; Farinon, S; Lo Vetere, M; Macri, M; Monge, M R; Musenich, R; Pallavicini, M; Parodi, R; Passaggio, S; Pastore, F C; Patrignani, C; Pia, M G; Priano, C; Robutti, E; Santroni, A; Morii, M; Bartoldus, R; Dignan, T; Hamilton, R; Mallik, U; Cochran, J; Crawley, H B; Fischer, P A; Lamsa, J; Meyer, W T; Rosenberg, E I; Benkebil, M; Grosdidier, G; Hast, C; Höcker, A; Lacker, H M; LePeltier, V; Lutz, A M; Plaszczynski, S; Schune, M H; Trincaz-Duvoid, S; Valassi, A; Wormser, G; Bionta, R M; Brigljevic, V; Fackler, O; Fujino, D; Lange, D J; Mugge, M; Shi, X; van Bibber, K; Wenaus, T J; Wright, D M; Wuest, C R; Carroll, M; Fry, J R; Gabathuler, E; Gamet, R; George, M; Kay, M; Payne, D J; Sloane, R J; Touramanis, C; Aspinwall, M L; Bowerman, D A; Dauncey, P D; Egede, U; Eschrich, I; Gunawardane, N J; Martin, R; Nash, J A; Sanders, P; Smith, D; Azzopardi, D E; Back, J J; Dixon, P; Harrison, P F; Potter, R J; Shorthouse, H W; Strother, P; Vidal, P B; Williams, M I; Cowan, G; George, S; Green, M G; Kurup, A; Marker, C E; McGrath, P; McMahon, T R; Ricciardi, S; Salvatore, F; Scott, I; Vaitsas, G; Brown, D; Davis, C L; Allison, J; Barlow, R J; Boyd, J T; Forti, A; Fullwood, J; Jackson, F; Lafferty, G D; Savvas, N; Simopoulos, E T; Weatherall, J H; Farbin, A; Jawahery, A; Lillard, V; Olsen, J; Roberts, D A; Schieck, J R; Blaylock, G; Dallapiccola, C; Flood, K T; Hertzbach, S S; Kofler, R; Lin, C S; Moore, T B; Staengle, H; Willocq, S; Wittlin, J; Brau, B; Cowan, R; Sciolla, G; Taylor, F; Yamamoto, R K; Britton, D I; Milek, M; Patel, P M; Trischuk, J; Lanni, F; Palombo, F; Bauer, J M; Booke, M; Cremaldi, L; Eschenburg, V; Kroeger, R; Reidy, J; Sanders, D A; Summers, D J; Martin, J P; Nief, J Y; Seitz, R; Taras, P; Zacek, V; Nicholson, H; Sutton, C S; Cartaro, C; Cavallo, N; De Nardo, G; Fabozzi, F; Gatto, C; Lista, L; Paolucci, P; Piccolo, D; Sciacca, C; LoSecco, J M; Alsmiller, J R; Gabriel, T A; Handler, T; Brau, J; Frey, R; Iwasaki, M; Sinev, N B; Strom, D; Colecchia, F; Dal Corso, F; Dorigo, A; Galeazzi, F; Margoni, M; Michelon, G; Morandin, M; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Torassa, E; Voci, C; Benayoun, M; Briand, H; Chauveau, J; David, P; De La Vaissière, C; Del Buono, L; Hamon, O; Le Diberder, F; Leruste, P; Lory, J; Roos, L; Stark, J; Versillé, S; Manfredi, P F; Re, V; Speziali, V; Frank, E D; Gladney, L; Guo, Q H; Panetta, J H; Angelini, C; Batignani, G; Bettarini, S; Bondioli, M; Carpinelli, M; Forti, F; Giorgi, M A; Lusiani, A; Martinez-Vidal, F; Morganti, M; Neri, N; Paoloni, E; Rama, M; Rizzo, G; Sandrelli, F; Simi, G; Triggiani, G; Walsh, J; Haire, M; Judd, D; Paick, K; Turnbull, L; Wagoner, D E; Albert, J; Bula, C; Lu, C; McDonald, K T; Miftakov, V; Schaffner, S F; Smith, A J; Tumanov, A; Varnes, E W; Cavoto, G; del Re, D; Faccini, R; Ferrarotto, F; Ferroni, F; Fratini, K; Lamanna, E; Leonardi, E; Mazzoni, M A; Morganti, S; Piredda, G; Safai Tehrani, F; Serra, M; Voena, C; Christ, S; Waldi, R; Adye, T; Franek, B; Geddes, N I; Gopal, G P; Xella, S M; Aleksan, R; De Domenico, G; Emery, S; Gaidot, A; Ganzhur, S F; Giraud, P F; Hamel De Monchenault, G; Kozanecki, W; Langer, M; London, G W; Mayer, B; Serfass, B; Vasseur, G; Yeche, C; Zito, M; Copty, N; Purohit, M V; Singh, H; Yumiceva, F X; Adam, I; Anthony, P L; Aston, D; Baird, K; Bartelt, J; Bloom, E; Boyarski, A M; Bulos, F; Calderini, G; Claus, R; Convery, M R; Coupal, D P; Coward, D H; Dorfan, J; Doser, M; Dunwoodie, W; Field, R C; Glanzman, T; Godfrey, G L; Grosso, P; Himel, T; Huffer, M E; Innes, W R; Jessop, C P; Kelsey, M H; Kim, P; Kocian, M L; Langenegger, U; Leith, D W; Luitz, S; Luth, V; Lynch, H L; Manzin, G; Marsiske, H; Menke, S; Messner, R; Moffeit, K C; Mount, R; Muller, D R; O'Grady, C P; Petrak, S; Quinn, H; Ratcliff, B N; Robertson, S H; Rochester, L S; Roodman, A; Schietinger, T; Schindler, R H; Schwiening, J; Serbo, V V; Snyder, A; Soha, A; Spanier, S M; Stahl, A; Stelzer, J; Su, D; Sullivan, M K; Talby, M; Tanaka, H A; Trunov, A; Va'vra, J; Wagner, S R; Weinstein, A J; Wisniewski, W J; Young, C C; Burchat, P R; Cheng, C H; Kirkby, D; Meyer, T I; Roat, C; De Silva, A; Henderson, R; Bugg, W; Cohn, H; Hart, E; Weidemann, A W; Benninger, T; Izen, J M; Kitayama, I; Lou, X C; Turcotte, M; Bianchi, F; Bona, M; Di Girolamo, B; Gamba, D; Smol, A; Zanin, D; Bosisio, L; Della Ricca, G; Lanceri, L; Pompili, A; Poropat, P; Prest, M; Vallazza, E; Vuagnin, G; Panvini, R S; Brown, C M; Kowalewski, R; Roney, J M; Band, H R; Charles, E; Dasu, S; Elmer, P; Hu, H; Johnson, J R; Liu, R; Nielsen, J; Orejudos, W; Pan, Y; Prepost, R; Scott, I J; Sekula, S J; von Wimmersperg-Toeller, J H; Wu, S L; Yu, Z; Zobering, H; Kordich, T M; Neal, H

2001-10-08

We have observed the decays B--> phiK and phiK(*) in a sample of over 45 million B mesons collected with the BABAR detector at the PEP-II collider. The measured branching fractions are B(B+--> phiK+) = (7.7(+1.6)(-1.4)+/-0.8)x10(-6), B(B0--> phiK0) = (8.1(+3.1)(-2.5)+/-0.8)x10(-6), B(B+--> phiK(*+)) = (9.7(+4.2)(-3.4)+/-1.7)x10(-6), and B(B0--> phiK(*0)) = (8.7(+2.5)(-2.1)+/-1.1)x10(-6). We also report the upper limit B(B+--> phipi(+))<1.4x10(-6) ( 90% C.L.).

Development of a fast, lean and agile direct pelletization process using experimental design techniques.

Science.gov (United States)

Politis, Stavros N; Rekkas, Dimitrios M

2017-04-01

A novel hot melt direct pelletization method was developed, characterized and optimized, using statistical thinking and experimental design tools. Mixtures of carnauba wax (CW) and HPMC K100M were spheronized using melted gelucire 50-13 as a binding material (BM). Experimentation was performed sequentially; a fractional factorial design was set up initially to screen the factors affecting the process, namely spray rate, quantity of BM, rotor speed, type of rotor disk, lubricant-glidant presence, additional spheronization time, powder feeding rate and quantity. From the eight factors assessed, three were further studied during process optimization (spray rate, quantity of BM and powder feeding rate), at different ratios of the solid mixture of CW and HPMC K100M. The study demonstrated that the novel hot melt process is fast, efficient, reproducible and predictable. Therefore, it can be adopted in a lean and agile manufacturing setting for the production of flexible pellet dosage forms with various release rates easily customized between immediate and modified delivery.

Development of a calorimetric system for electron beam dosimetry in radiation processing

International Nuclear Information System (INIS)

Banados P, H.E.

1994-01-01

A calorimetric system for electron beam dosimetry in radiation processing was developed. The system is composed of a graphite core calorimeter, the temperature measuring and electrical calibrating instrumentation, a microcomputer and the software for the system automation. The research aimed at the optimization of the project parameters, the development of advanced methodologies for calibrating the temperature sensor, the determination of the thermal capacity as a function of the temperature, the measurement of the absorbed dose, and the development of the software needed for the system operation. The operating range extends from 0.1 kGy to 30 kGy. The uncertainty in the measurement of the absorbed dose was estimated to be ± 1.8% at the 95% confidence level. Comparative tests of the absorbed dose measurements were made using the IPEN electron accelerator. The results obtained showed an excellent agreement between the absorbed dose determined by the calorimeter and the absorbed dose calculated from the nominal power delivered by the accelerator. (author). 67 refs, 63 figs, 2 tabs

Thermodynamic study of 9-anthracenecarboxylic acid

International Nuclear Information System (INIS)

Ribeiro da Silva, Manuel A.V.; Santos, Ana Filipa L.O.M.; Carneiro, Liliana P.T.; Mendes, Ricardo F.; Rodrigues, Ana Sofia M.C.; Ferreira, Paulo J.O.; Ramos, Rui M.C.

2011-01-01

The standard massic energy of combustion, in oxygen, of the crystalline 9-anthracenecarboxylic acid was measured, at T = 298.15 K, by static-bomb combustion calorimetry, from which the standard molar enthalpy of formation, in the condensed phase, was calculated as Δ f H m 0 (cr)=-(267.3±3.4)kJ.mol -1 . The standard molar enthalpy of sublimation of this acid, at T = 298.15 K, Δ cr g H m 0 =(139.4±0.9)kJ.mol -1 , was determined from the temperature-vapour pressure dependence, obtained by the Knudsen mass-loss effusion method. From the data presented above, the standard molar enthalpy of formation, in the gaseous phase, at T = 298.15 K, was derived, Δ f H m 0 (g)=-(127.9±3.5)kJ.mol -1 . The experimental result is interpreted in terms of enthalpic increments, molecular structure and compared with structurally similar compounds.

Polyvalent horse F(Ab`)2 snake antivenom: Development of process ...

African Journals Online (AJOL)

USER

2010-04-19

Apr 19, 2010 ... African Journal of Biotechnology Vol. 9 (16), pp. ... filtered through a 30 kDa ultrafiltration membrane. F(ab´)2 .... was added to the plasma pool obtained from the horses immunized with whole ..... revision of the manuscript.

Structural investigation on K3Gd5(PO4)6 in between 20 K to 1073 K

International Nuclear Information System (INIS)

Bevara, Samatha; Achary, S.N.; Tyagi, A.K.; Mishra, K.K.; Ravindran, T.R.; Sinha, A.K.; Sastry, P.U.

2016-01-01

Evolution of crystal structure of K 3 Gd 5 (PO 4 ) 6 in the temperature range from 20 K to 1073 K, as observed from combined variable temperature X-ray diffraction (using both synchrotron source and Cu K α lab source) and Raman spectroscopic studies is communicated in the manuscript. The title compound has an open tunnel containing three dimensional structure built by periodic arrangements of (Gd 5 (PO 4 ) 6 ) 3- ions which in turn are formed by PO 4 tetrahedra and GdO n (n = 8 and 9) polyhedra and these tunnels are occupied by K + ions. The XRD patterns in the entire temperature range of study indicated no change in the crystal structural, which is also supported by differential thermal analyses and Raman spectroscopy. Average axial thermal expansion coefficients between 20K and 1073 K are : α a =10.6 x 10 -6 K -1 , α b = 5.5 x 10 -6 K -1 and α c = 16.4 X 10.6 -6 K -1 . (author)

Development of a 200kW multi-fuel type PAFC power plant

Energy Technology Data Exchange (ETDEWEB)

Take, Tetsuo; Kuwata, Yutaka; Adachi, Masahito; Ogata, Tsutomu [NTT Integrated Information & Energy System Labs., Tokyo (Japan)

1996-12-31

Nippon Telegraph and Telephone Corporation (NFT) has been developing a 200 kW multi-fuel type PAFC power plant which can generate AC 200 kW of constant power by switching fuel from pipeline town gas to liquefied propane gas (LPG) and vice versa. This paper describes the outline of the demonstration test plant and test results of its fundamental characteristics.

Scale up risk of developing oil shale processing units

International Nuclear Information System (INIS)

Oepik, I.

1991-01-01

The experiences in oil shale processing in three large countries, China, the U.S.A. and the U.S.S.R. have demonstrated, that the relative scale up risk of developing oil shale processing units is related to the scale up factor. On the background of large programmes for developing the oil shale industry branch, i.e. the $30 billion investments in colorado and Utah or 50 million t/year oil shale processing in Estonia and Leningrad Region planned in the late seventies, the absolute scope of the scale up risk of developing single retorting plants, seems to be justified. But under the conditions of low crude oil prices, when the large-scale development of oil shale processing industry is stopped, the absolute scope of the scale up risk is to be divided between a small number of units. Therefore, it is reasonable to build the new commercial oil shale processing plants with a minimum scale up risk. For example, in Estonia a new oil shale processing plant with gas combustion retorts projected to start in the early nineties will be equipped with four units of 1500 t/day enriched oil shale throughput each, designed with scale up factor M=1.5 and with a minimum scale up risk, only r=2.5-4.5%. The oil shale retorting unit for the PAMA plant in Israel [1] is planned to develop in three steps, also with minimum scale up risk: feasibility studies in Colorado with Israel's shale at Paraho 250 t/day retort and other tests, demonstration retort of 700 t/day and M=2.8 in Israel, and commercial retorts in the early nineties with the capacity of about 1000 t/day with M=1.4. The scale up risk of the PAMA project r=2-4% is approximately the same as that in Estonia. the knowledge of the scope of the scale up risk of developing oil shale processing retorts assists on the calculation of production costs in erecting new units. (author). 9 refs., 2 tabs

SSC-K code users manual (rev.1)

International Nuclear Information System (INIS)

Kwon, Y. M.; Lee, Y. B.; Chang, W. P.; Hahn, D.

2002-01-01

The Supper System Code of KAERI (SSC-K) is a best-estimate system code for analyzing a variety of off-normal or accidents in the heat transport system of a pool type LMR design. It is being developed at Korea Atomic Energy Research Institution (KAERI) on the basis of SSC-L, originally developed at BNL to analyze loop-type LMR transients. SSC-K can handle both designs of loop and pool type LMRs. SSC-K contains detailed mechanistic models of transient thermal, hydraulic, neutronic, and mechanical phenomena to describe the response of the reactor core, coolant, fuel elements, and structures to accident conditions. This report provides a revised User's Manual (rev.1) of the SSC-K computer code, focusing on phenomenological model descriptions for new thermal, hydraulic, neutronic, and mechanical modules. A comprehensive description of the models for pool-type reactor is given in Chapters 2 and 3; the steady-state plant characterization, prior to the initiation of transient is described in Chapter 2 and their transient counterparts are discussed in Chapter 3. Discussions on the intermediate heat exchanger (IHX) and the electromagnetic (EM) pump are described in Chapter 4 and 5, respectively. A model of passive safety decay heat removal system (PSDRS) is discussed in Chapter 6, and models for various reactivity feedback effects are discussed in Chapter 7. In Chapter 8, constitutive laws and correlations required to execute the SSC-K are described. New models developed for SSC-K rev.1 are two dimensional hot pool model in Chapter 9, and long term cooling model in Chapter 10. Finally, a brief description of MINET code adopted to simulate BOP is presented in Chapter 11. Based on test runs for typical LMFBR accident analyses, it was found that the present version of SSC-K would be used for the safety analysis of KALIMER. However, the further validation of SSC-K is required for real applications. It is noted that the user's manual of SSC-K will be revised later with the

76 FR 45825 - Center for Devices and Radiological Health 510(k) Clearance Process; Institute of Medicine Report...

Science.gov (United States)

2011-08-01

... Drug Administration (FDA) is requesting comments on the Institute of Medicine (IOM) report entitled... assessment of the 510(k) process was an independent study by the IOM. At the request of FDA, IOM has..., 2011, IOM released the report ``Medical Devices and the Public's Health, The FDA 510(k) Clearance...

H3K9me-independent gene silencing in fission yeast heterochromatin by Clr5 and histone deacetylases

DEFF Research Database (Denmark)

Hansen, Klavs R; Hazan, Idit; Shanker, Sreenath

2011-01-01

organisms such as fission yeast. In spite of numerous studies, the relative contributions of the various heterochromatic histone marks to the properties of heterochromatin remain largely undefined. Here, we report that silencing of the fission yeast mating-type cassettes, which are located in a well......, our results point to histone deacetylases as prominent repressors of gene expression in fission yeast heterochromatin. These deacetylases can act in concert with, or independently of, the widely studied H3K9me mark to influence gene silencing at heterochromatic loci....

Development of an ultrasonic process for soil remediation

International Nuclear Information System (INIS)

Wu, J.M.; Huang, H.S.; Livengood, C.D.

1995-01-01

An ultrasonic process for the detoxification of carbon tetrachloride- (CCl 4 - ) contaminated soil was investigated in the laboratory by using a batch irradiation reactor equipped with a 600-W ultrasonic power supply operated at a frequency of 20 kHz. Key parameters studied included soil characteristics, irradiation time, CCl 4 concentration, steady-state operating temperature, applied ultrasonic-wave energy, and the ratio of soil to water in the system. The results of the experiments showed that (1) residual CCl 4 concentrations could be decreased with longer irradiation periods and (2) detoxification efficiency was proportional to steady-state operating temperature and applied ultrasonic-wave energy. The characteristics of the contaminated soil were found to be an important factor in the design of an ultrasonic detoxification system. A soil-phase CCl 4 concentration below 1 ppm (initial concentration of 56 ppm) was achieved through this process, indicating that the application of ultrasonic irradiation is feasible and effective in the detoxification of soil contaminated by organic compounds. On the basis of the experimental results, a schematic of a full-scale ultrasonic soil-detoxification system was developed. Improvements to this novel process are discussed

A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus

Directory of Open Access Journals (Sweden)

Crumpacker Clyde S

2011-01-01

Full Text Available Abstract Background The major hurdle in the treatment of Human Immunodeficiency virus type 1 (HIV-1 includes the development of drug resistance-associated mutations in the target regions of the virus. Since reverse transcriptase (RT is essential for HIV-1 replication, several nucleoside analogues have been developed to target RT of the virus. Clinical studies have shown that mutations at RT codon 65 and 74 which are located in β3-β4 linkage group of finger sub-domain of RT are selected during treatment with several RT inhibitors, including didanosine, deoxycytidine, abacavir and tenofovir. Interestingly, the co-selection of K65R and L74V is rare in clinical settings. We have previously shown that K65R and L74V are incompatible and a R→K reversion occurs at codon 65 during replication of the virus. Analysis of the HIV resistance database has revealed that similar to K65R+L74V, the double mutant K65R+L74I is also rare. We sought to compare the impact of L→V versus L→I change at codon 74 in the background of K65R mutation, on the replication of doubly mutant viruses. Methods Proviral clones containing K65R, L74V, L74I, K65R+L74V and K65R+L74I RT mutations were created in pNL4-3 backbone and viruses were produced in 293T cells. Replication efficiencies of all the viruses were compared in peripheral blood mononuclear (PBM cells in the absence of selection pressure. Replication capacity (RC of mutant viruses in relation to wild type was calculated on the basis of antigen p24 production and RT activity, and paired analysis by student t-test was performed among RCs of doubly mutant viruses. Reversion at RT codons 65 and 74 was monitored during replication in PBM cells. In vitro processivity of mutant RTs was measured to analyze the impact of amino acid changes at RT codon 74. Results Replication kinetics plot showed that all of the mutant viruses were attenuated as compared to wild type (WT virus. Although attenuated in comparison to WT virus

Development of X-ray radiography examination technology by image processing method

Energy Technology Data Exchange (ETDEWEB)

Min, Duck Kee; Koo, Dae Seo; Kim, Eun Ka [Korea Atomic Energy Research Institute, Taejon (Korea)

1998-06-01

Because the dimension of nuclear fuel rods was measured with rapidity and accuracy by X-ray radiography examination, the set-up of image processing system which was composed of 979 CCD-L camera, image processing card and fluorescent lighting was carried out, and the image processing system enabled image processing to perform. The examination technology of X-ray radiography, which enabled dimension measurement of nuclear fuel rods to perform, was developed by image processing method. The result of dimension measurement of standard fuel rod by image processing method was 2% reduction in relative measuring error than that of X-ray radiography film, while the former was better by 100 {approx} 200 {mu}m in measuring accuracy than the latter. (author). 9 refs., 22 figs., 3 tabs.

Study of charmonium resonances in the γγ -> K⁰_SK^± π^∓i- and γγ -> K⁺ K^-π⁺π^-π⁰ processes

Energy Technology Data Exchange (ETDEWEB)

Biassoni, Pietro [Univ. of Milan (Italy)

2011-01-01

This thesis reports the analysis of the e⁺e^- → e⁺e^-K_S⁰K^±π^± and e⁺e^- → e⁺e^-K⁺K^-π⁺π^-π⁰ processes using the final dataset of the BABAR experiment located at the SLAC National Accelerator Laboratory. From previous measurements, the K_S⁰K^±π^± final state is known to show a clear signal from the β_c(2S) particle. This c$\\bar{c}$ state escaped detection for almost twenty years and its properties are still not well established on the experimental ground, while accurate predictions exist on the theoretical side. The e⁺e^-→ e⁺e^-K⁺K^-π⁺π^-π⁰ process is first studied in this thesis. An accurate determination of the β_c(2S) properties is obtained in the K_S⁰K^±π^± decay mode. We also report the first observation of η_c(2S) and other charmonium states to the K⁺K^-π⁺π^-π⁰ final state. The results of this thesis have been published in Physical Review D, and will be useful to test theoretical models describing the charmonium system. The thesis is organized in four chapters. The first one gives a brief introduction of the theoretical models used to describe the charmonium system. The second one discuss the current status of conventional and exotic charmonium spectroscopy, reporting recent experimental results and their interpretation. The third Chapter is devoted to describe the BABAR experiment. The analysis technique and results are described in Chapter 4. Finally, conclusions from this analysis are drawn.