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Sample records for prevent potato sprouting

  1. Environmental life cycle analysis of potato sprout inhibitors

    NARCIS (Netherlands)

    Kerstholt, R.P.V.; Ree, C.M.; Moll, H.C.

    Potato sprout inhibitors are generally applied to suppress sprouting during winter storage. This study presents the compared environmental profiles of the two sprout inhibitors available on the Dutch market: A traditional chemical product with isopropyl-3-chlorophenylcarbamate (CIPC) and

  2. Sprout inhibition of potatoes by gamma irradiation

    International Nuclear Information System (INIS)

    Pringsulaka, Vachira.

    1982-08-01

    Harvested fresh potatoes of Spunta variety were irradiated at 0, 60, 90, 120 and 150 gray and then stored at three different temperatures; room temperature (30+-5degC) with R.H. 55-65%, 15degC and 10degC with R.H. 85-95%. The percentage of sprout, rot, and weight loss were investigated every two weeks for six months. Both non-irradiated and irradiated potatoes stored at room temperature with R.H. 55-65% showed higher percentage in rottenint than in sprouting. All of irradiated potatoes stored at 15degC and 10degC with R.H. 85-95% sprouted after 3 months and at 4 months respectively. However, only 2.54% and 1.27% of sprout were found in 150 gray of irradiated potatoes stored at 15degC and 10degC with R.H. 85-95% respectively. No significant difference was found in weighted loss and in texture between irradiated and non-irradiated potatoes under the same condition. There is no significant difference in organoleptic test (P<0.05) between the irradiated and non-irradiated potatoes in all doses

  3. Regulation of potato tuber dormancy and sprouting

    NARCIS (Netherlands)

    Aksenova, N.P.; Sergeeva, L.; Konstantinova, T.N.; Golyanovskaya, S.A.; Kolachevskaya, O.O.; Romanov, G.A.

    2013-01-01

    Dormancy is the final stage of tuber life serving to preserve tubers as organs of vegetative reproduction under unfavorable growth conditions. Since the duration of potato tuber dormancy and their sprouting time have significant economic importance, much attention is given to the study of the

  4. Virus free seed potato production through sprout cutting technique ...

    African Journals Online (AJOL)

    In order to evaluate the performance of sprout cutting for seed potato production against virus infection, a study was conducted at Bangladesh Agricultural Development Corporation (BADC) Foundation Seed Potato Production Farm, Domar, Nilphamary, Bangladesh in 2005 - 2006. Sprout cut seedlings were grown under ...

  5. Evaluation of citrus, butternut and sprouting potato as mass rearing ...

    African Journals Online (AJOL)

    P. burnerae required 666.7 degree-days on citrus and 434.8 degree-days on sprouting potatoes which is above lower developmental thresholds of 7.6 and 10.4°C, respectively, to complete one generation. The mean number of eggs per female was higher on sprouting potatoes (121.3) than on citrus (68), but declined with ...

  6. Extraction and isolation of alpha-solanine from potato sprouts

    OpenAIRE

    Spoladore, Dayse Soave; Zullo, Marco Antonio Teixeira; Teixeira, João Paulo Feijão

    1983-01-01

    Fresh potato sprouts were extracted with 2% aqueous acetic acid for 48 hours. The crude alkaloidal extract was purified by repeating the operation. Precipitation by concentrated ammonium hydroxide, centrifugation, dissolution of the resulting precipitate in 2% acetic acid, till a clear alkaline supernatant was obtained. The alpha-solanine, obtained by this procedure was purified by successive recristalizations with ethyl alcohol. The total yield was about 0.02% of the fresh material, with pur...

  7. Catalase inhibition accelerates dormancy release and sprouting in potato (Solanum tuberosum L.) tubers

    OpenAIRE

    Mohammed Bajji; Mahmoud M’Hamdi; Frédéric Gastiny; Jorge A. Rojas-Beltran; Patrick du Jardin

    2007-01-01

    The involvement of hydrogen peroxide (H2O2) metabolism in dormancy release and sprouting of potato (Solanum tuberosum L.) tubers has been investigated using three complementary approaches. In the first approach, the evolution of the sprouting kinetics, H2O2 content and antioxidant enzyme activities were examined during tuber storage. The most important changes occurred at the « bud/sprout » level. In particular, dormancy release was accompanied by a transient but remarkable increase in H2O2 c...

  8. Different aspects of S-carvone, a natural potato sprout growth inhibitor

    NARCIS (Netherlands)

    Oosterhaven, J.

    1995-01-01

    After harvest, potato tubers are usually stored at a temperature of 6-8°C in combination with the application of a synthetic sprout inhibitor. Frequently used sprout inhibitors are isopropyl N-phenyl-carbamate (propham or IPC), isopropyl N-(3-chlorophenyl)carbamate (chlorpropham or CIPC) or

  9. Infection with Rhizoctonia solani induces defense genes and systemic resistance in potato sprouts grown without light.

    Science.gov (United States)

    Lehtonen, M J; Somervuo, P; Valkonen, J P T

    2008-11-01

    Rhizoctonia solani is an important soilborne and seedborne fungal pathogen of potato (Solanum tuberosum). The initial infection of sprouts prior to emergence causes lesions and may be lethal to the sprout or sprout tip, which results in initiation and compensatory growth of new sprouts. They emerge successfully and do not suffer significant damage. The mechanism behind this recovery phenomenon is not known. It was hypothesized that infection may induce pathogen defense in sprouts, which was investigated in the present study. Tubers were sprouted in cool and moist conditions in darkness to mimic conditions beneath soil. The basal portion of the sprout was isolated from the apical portion with a soft plastic collar and inoculated with highly virulent R. solani. Induction of defense-related responses was monitored in the apical portion using microarray and quantitative polymerase chain reaction techniques at 48 and 120 h postinoculation (hpi) and by challenge-inoculation with R. solani in two experiments. Differential expression of 122 and 779 genes, including many well-characterized defense-related genes, was detected at 48 and 120 hpi, respectively. The apical portion of the sprout also expressed resistance which inhibited secondary infection of the sprouts. The observed systemic induction of resistance in sprouts upon infection with virulent R. solani provides novel information about pathogen defense in potato before the plant emerges and becomes photosynthetically active. These results advance our understanding of the little studied subject of pathogen defense in subterranean parts of plants.

  10. Catalase inhibition accelerates dormancy release and sprouting in potato (Solanum tuberosum L. tubers

    Directory of Open Access Journals (Sweden)

    Mohammed Bajji

    2007-01-01

    Full Text Available The involvement of hydrogen peroxide (H2O2 metabolism in dormancy release and sprouting of potato (Solanum tuberosum L. tubers has been investigated using three complementary approaches. In the first approach, the evolution of the sprouting kinetics, H2O2 content and antioxidant enzyme activities were examined during tuber storage. The most important changes occurred at the « bud/sprout » level. In particular, dormancy release was accompanied by a transient but remarkable increase in H2O2 content. In the second approach, the effect of a catalase (CAT, EC 1.11.1.6 inhibitor (thiourea or of exogenous H2O2 application on tuber sprouting behaviour was assessed. Both treatments resulted in a reduction of the dormancy period and in rapid and synchronised sprouting of the treated tubers when compared to the control as well as in increased sprout number per tuber. In the third approach, the effect of CAT inhibition on potato tuber dormancy and sprouting was evaluated using the transgenic technology. Plants partially repressed in their CAT activity were produced and, once again, CAT inhibition resulted in acceleration of the sprouting kinetics and in increased sprout number of the transgenic tubers compared to those from the wild type. It thus appears that tuber dormancy and sprouting can be controlled in potato by the manipulation of H2O2 metabolism via the inhibition of CAT activity. The possible mechanisms whereby CAT inhibitors or H2O2 overcome dormancy and promote sprouting in the potato tuber are discussed in relation to what is known in other plant models (seeds and fruit tree buds.

  11. The irradiation dose for the inhibition of the sprouting of Baraka variety potatoes

    International Nuclear Information System (INIS)

    Perez Rivero, B.; Salcines, R.; Prieto, E.

    1990-01-01

    Baraka variety potatoes graded in the packing house, were irradiated with doses of 0.08; 0.10; 0.15 and 0.20 kGy after a two weeks curing period. The potatoes were stored for five months at 12 0 +- 2 0 C and relative humidity of 85 to 95%, and to determine the percentages of sprouting, rotting and loss in weight. It was concluded that doses of 0.08 kGy or more inhibited definitively the sprouting process. Less total losses and better commercial quality were obtained with a dose of 0.08 kGy the last of the store. 13 refs

  12. Preemergence infection of potato sprouts by Phytophthora infestans in the highland tropics of Ecuador

    DEFF Research Database (Denmark)

    Kromann, Peter; Taipe, Arturo; Andrade-Piedra, Jorge L.

    2008-01-01

    and 250 sporangia/ml) daily for 10 days to the soil surface of pots planted with sprouted seed potato tubers. The daily inoculation rate of 50 sporangia/ml (15.9 × 103 sporangia/m2) resulted in sprout infection in 100% of inoculated pots and roughly corresponded to the sporangial deposition accumulated......, which had been removed from soil prior to emergence. Tubers had been planted within 10 m of approximately 300-m2 plots with mature potato plants severely infected with late blight. Infection potential of potato sprouts also was evaluated in the greenhouse by applying 10-ml sporangial suspensions (50...

  13. Carbohydrate metabolism during potato tuber dormancy and sprouting

    NARCIS (Netherlands)

    Claassens, M.M.J.

    2002-01-01

    Potato tuber dormancy is part of the vegetative life cycle of potato. It refers to a period, in which no growth is occurring, although the tuber is stored under conditions that are favourable for growth. Factors in the tuber are responsible for this growth arrest; soon

  14. Sprout inhibition and change in organic components of potato by gamma-irradiation

    International Nuclear Information System (INIS)

    Rahman, M.S.; Kume, Tamikazu; Ishigaki, Isao.

    1989-12-01

    Radiation technology for sprout inhibition and change in organic components of potato by irradiation were investigated. Dose distribution in the package filled with potatoes (depth 45cm, density: 0.56g/cm 3 ) was measured using Fricke dosimeter. When the package was irradiated at dose rate of 5 x 10 5 , 1 x 10 5 and 5 x 10 4 rad/hr, the dose uniformities were calculated as 1.79, 1,45 and 1.35, and the relative throughput capacities were 1.0, 0.26 and 0.14, respectively. After 7 months storage, the sprout of potatoes was not observed at 10 krad irradiation while 57% of potatoes was sprouted at 5 krad. The contents of oxalic and malic acids were slightly increased by irradiation up to 100 krad while that of citric and succinic acids were not changed. The change in contents of these organic acids during storage was almost the same in both unirradiated and irradiated samples. Sucrose content was reached maximum after 8 days in 15 krad irradiated sample while it was increased through 40 days storage in 300 krad irradiated sample. The increase in sucrose contents by irradiation was higher in cortical tissue than in medullary tissue. (author)

  15. Effect of sprouting on anthocyanin, antioxidant activity, color intensity and color attributes in purple sweet potatoes

    Directory of Open Access Journals (Sweden)

    Yudiono, K.

    2017-11-01

    Full Text Available Sweet potatoes stored in a humid state will generally sprout, leading to them being deemed inedible by customers. During the sprouting process enzyme activities increase and as such it was assumed that there had been changes in the nutrition and secondary metabolism compounds, e.g. anthocyanins. The purpose of this research was to investigate the changes occurred in the characteristics of anthocyanins during the purple sweet potatoes’ sprouting. One-factor Randomized Block Design was used in the research design, i.e. the sprouting time in 6 stages: 1, 2, 3, 4, 5, and 6 weeks. The results showed that the highest antioxidant activity is 51.61±2.11%, the highest color intensity is 0.72±0.72, and the lowest hue angle value or the lowest h is 30.75±0.93o, occurring in the 4th week of sprouting. While the highest total anthocyanin is 222.07±2.65 mg/kg wet weight, and the highest red color value or a* is 12.80±0.49, occurring in the 3rd week of sprouting. The major components that compose purple sweet potatoes are Cyanidin-3-0-glucoside ([M-X]+ = m/z 286.50 to 287.50 and [M]+ = m/z 449 and Peonidin -3,5-0-diglucoside ([M-X]+ = m/z 462.50 to 463.50 and [M]+ = m/z 625.

  16. Physiological mechanisms for potato dormancy release and sprouting

    African Journals Online (AJOL)

    Potato (Solanum tuberosum L.) tubers undergo a period of dormancy, during which visible bud growth is inhibited. The length of the dormancy is determined by environmental, physiological and hormonal control mechanisms. Dormancy is the final stage of tuber life, which serves to preserve tubers as organs of vegetative ...

  17. Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern

    NARCIS (Netherlands)

    Delaplace, P.; Fauconnier, M.L.; Sergeant, K.; Dierick, J.F.; Oufir, M.; Wal, van der F.; America, A.H.P.; Renaut, J.; Hausman, J.F.; Jardin, du P.

    2009-01-01

    During post-harvest storage, potato tubers age as they undergo an evolution of their physiological state influencing their sprouting pattern. In the present study, physiological and biochemical approaches were combined to provide new insights on potato (Solanum tuberosum L. cv. Desiree) tuber

  18. The combined effect of CO2 and ethylene sprout inhibitor on the fry colour of stored potatoes (Solanum tuberosum L.)

    NARCIS (Netherlands)

    Daniels-Lake, B.J.

    2013-01-01

    Recently, it has been shown that the darkening of potato processing colour attributable to a trace concentration of ethylene gas is more severe when CO2 is also elevated. In view of the increasing use of ethylene gas for sprout suppression in potato storage facilities, it was considered important to

  19. Chemical diversity in Mentha spicata: antioxidant and potato sprout inhibition activity of its essential oils.

    Science.gov (United States)

    Chauhan, Shailendra S; Prakash, Om; Padalia, Rajendra C; Vivekanand; Pant, Anil K; Mathela, Chandra S

    2011-09-01

    The essential oils from fresh aerial parts of Mentha spicata L. collected from ten different natural habitats of Uttarakhand, India were analyzed by a combination of GC, GC/MS and NMR spectroscopy. The analysis revealed that monoterpenoids (46.1%-91.6%), mainly carvone (15.3%-68.5%), piperetenone oxide (24.0%-79.2%) and alpha-humulene (0.1%-29.9%), were the major constituents of the essential oils, but with significant qualitative and quantitative differences among the other constituents. Cluster analysis of the oil composition was carried out in order to discern the differences and similarities within different accessions collected from different natural habitats. The essential oils were also screened for their antioxidant activities by chelating properties of Fe2+, DPPH radical-scavenging activity, and their reducing power. The essential oils of two chemo variants (viz. carvone and piperetenone oxide types) were also tested for potato (Solanum tuberosum L.) sprout suppressant activity. The results showed that these oils exhibit good sprout inhibition activity in comparison to CIPC and iodine, the standard sprout suppressant.

  20. Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern

    OpenAIRE

    Delaplace, Pierre; Fauconnier, Marie-Laure; Sergeant, Kjell; Dierick, Jean-Fran?ois; Oufir, Mouhssin; van der Wal, Froukje; America, Antoine H. P.; Renaut, Jenny; Hausman, Jean-Fran?ois; du Jardin, Patrick

    2009-01-01

    During post-harvest storage, potato tubers age as they undergo an evolution of their physiological state influencing their sprouting pattern. In the present study, physiological and biochemical approaches were combined to provide new insights on potato (Solanum tuberosum L. cv. D?sir?e) tuber ageing. An increase in the physiological age index (PAI) value from 0.14 to 0.83 occurred during storage at 4??C over 270?d. Using this reference frame, a proteomic approach was followed based on two-dim...

  1. Potato Sprout Inhibition and Tuber Quality after Post-Harvest Treatment with Rosemary (Rosmarinus Officinalis L.) Leaves and Branches

    DEFF Research Database (Denmark)

    Talei, Daryush; Bina, Fatemeh; Valdiani, Alireza

    2017-01-01

    Storage of potatoes is one of the most important concerns in maintaining freshness and nutritional quality in the storage process. To achieve this, an experiment was carried out with five different storage conditions at various temperatures using fresh rosemary leaves and branches with three...... replicates. The results revealed that storage of potatoes at 25°C with rosemary leaves and branches resulted in the lowest sprout development and weight loss after 10 weeks. This was significantly different from either 4°C or 30°C. The findings indicated the potential of rosemary fresh leaves and branches...

  2. Studies on sprout inhibition of potatoes and onions and shelf-life extensions of dates in Iraq

    International Nuclear Information System (INIS)

    Auda, H.; Khalaf, Z.

    1979-01-01

    Small scale experiments were carried out with onions and potatoes to examine the effect of gamma irradiation on sprout inhibition under different storage conditions. Doses of 15 to 20 krad caused some physical damage to potatoes stored in an incubator at 4 to 10 0 C and 40 to 70% relative humidity. This damage was also observed when potatoes have been stored at room temperature. Sprouting appeared after 30 to 60 days storage. The best results were obtained when a dose of 10 to 12 krad was used. Studies on onions irradiated and stored at room temperature for ten months showed that 6 to 9 krad gave the best results, extending the storage for 10 months. No sprouting could be detected throughout the storage period. Shelf-life and organoleptic study was done on four varieties of Iraqi dates at different stages of development. The results showed that the eating quality of fresh dates was not affected significantly by irradiation up to 270 krad and the duration of softening process (after-ripening) of dates was prolonged by low doses of 10 to 90 krad in the majority of the experimental batches. (author)

  3. FT-ICR/MS and GC-EI/MS metabolomics networking unravels global potato sprout's responses to Rhizoctonia solani infection.

    Directory of Open Access Journals (Sweden)

    Konstantinos A Aliferis

    Full Text Available The complexity of plant-pathogen interactions makes their dissection a challenging task for metabolomics studies. Here we are reporting on an integrated metabolomics networking approach combining gas chromatography/mass spectrometry (GC/MS with Fourier transform ion cyclotron resonance/mass spectrometry (FT-ICR/MS and bioinformatics analyses for the study of interactions in the potato sprout-Rhizoctonia solani pathosystem and the fluctuations in the global metabolome of sprouts. The developed bioanalytical and bioinformatics protocols provided a snapshot of the sprout's global metabolic network and its perturbations as a result of pathogen invasion. Mevalonic acid and deoxy-xylulose pathways were substantially up-regulated leading to the biosynthesis of sesquiterpene alkaloids such as the phytoalexins phytuberin, rishitin, and solavetivone, and steroidal alkaloids having solasodine and solanidine as their common aglycons. Additionally, the perturbation of the sprout's metabolism was depicted in fluctuations of the content of their amino acids pool and that of carboxylic and fatty acids. Components of the systemic acquired resistance (SAR and hypersensitive reaction (HR such as azelaic and oxalic acids were detected in increased levels in infected sprouts and strategies of the pathogen to overcome plant defense were proposed. Our metabolic approach has not only greatly expanded the multitude of metabolites previously reported in potato in response to pathogen invasion, but also enabled the identification of bioactive plant-derived metabolites providing valuable information that could be exploited in biotechnology, biomarker-assisted plant breeding, and crop protection for the development of new crop protection agents.

  4. Mercury content of sprouts and harvested roots from treated sweet potato mother roots. [Ipomoea batatas

    Energy Technology Data Exchange (ETDEWEB)

    Huisingh, D.; Nielsen, L.W.

    1972-01-01

    Mercury containing fungicides have been used extensively for seed and root disease control, but data on the fate of the mercury (Hg) are scarce. Experiments were designed to see if Hg applied to propagative sweet potato roots increased the Hg-content of edible roots. Roots were treated with Semesan Bel(hydroxymercurinitrophenol + hydroxymercurichlorophenol), Mertect (Thiabendazole: 2-(4-Thiazolyl)-benzimidazole), or Botran (2,6-Dichloro-4-nitroanaline) at recommended rates or with water. Treated roots were bedded into sandy loam soil, and the plants were harvested at 2 and 3 months after bedding. Some sprouts transplanted at 2 months were grown to maturity, and the harvested roots were analyzed. Hg analyses were performed by flameless atomic absorption. Roots treated prior to planting with Semesan Bel, Mertect, Botran, and water contained 23.0, 0.05, 0.03 and 0.03 ..mu..g/g dry wt, respectively. At the 2-month harvest, the leaves and stems of the Semesan Bel-treated plants contained 5 times more Hg than those of the other treatments. By the 3-month harvest, the amount of Hg in plant leaves and stems from Hg-treated roots was 2 to 3 times that of the others. Fall harvested fleshy roots contained 0.03, 0.02, 0.03, and 0.03 ..mu..g/g dry wt Hg for the Semesan Bel, Mertect, Botran, and water treatments, respectively. This demonstrates that the Hg applied to the mother root was translocated to the new plant, but little if any was translocated to the new fleshy roots.

  5. Effect of red light radiation on survival and tuberisation of potato sprouts grown 'in vitro': role of physiological age

    International Nuclear Information System (INIS)

    Blanc, A.; Mery, J.C.; Boisard, J.

    1986-01-01

    Short spells (5 min) of red light applied to etiolated potato sprouts after excision (0 time) caused opposite effects on rate of tuberisation after the sprouts had been planted; tuberisation was speeded up by light red (RC) radiation and slowed down by dark red (RS) radiation. If the treatment was postponed to after subjecting the sprouts to darkness, RS effect was reduced whereas that of RC enhanced in relation to the duration of the dark period (Table 1). The effect of RS at 0 time could be explained in terms of the presence of a low level of preformed Pfr (active form of phytochrome) in the etiolated sprouts. The Pfr would disappear gradually in darkness resulting in the loss of efficiency of RS radiation and an increase in the efficiency of RC radiation. The marked effect of RS radiation at 0 time was even more evident if the sprouts were excised from physiologically older tubers (higher incubation temperature). The increase in related physiological responses —persistence and speed of tuberisation in vitro — could be explained by the development during incubation of conditions more favourable to the activity of the photoreceptor in the cells (Table 2). Red light treatment at low temperatures (Table 3) on mature tubers (Table 4) caused an increase in total phytochrome content in etiolated sprouts which would develop later on those tubers in the dark. Light appeared to control tuberisation through its action on an organ which detects the stimulus (tuber) to an organ derived from it (sprout) [de

  6. Differences between the Bud End and Stem End of Potatoes in Dry Matter Content, Starch Granule Size, and Carbohydrate Metabolic Gene Expression at the Growing and Sprouting Stages.

    Science.gov (United States)

    Liu, Bailin; Zhang, Guodong; Murphy, Agnes; De Koeyer, David; Tai, Helen; Bizimungu, Benoit; Si, Huaijun; Li, Xiu-Qing

    2016-02-10

    Potatoes usually have the tuber bud end dominance in growth during tuber bulking and in tuber sprouting, likely using carbohydrates from the tuber stem end. We hypothesized that the tuber bud end and tuber stem end coordination in carbohydrate metabolism gene expression is different between the bulking dominance and sprouting dominance of the tuber bud end. After comparing the growing tubers at harvest from a green vine and the stage that sprouts just started to emerge after storage of tubers at room temperature, we found the following: (1) Dry matter content was higher in the tuber stem end than the tuber bud end at both stages. (2) The starch granule size was larger in the tuber bud end than in the tuber stem end. (3) The tuber bud end had higher gene expression for starch synthesis but a lower gene expression of sucrose transporters than the tuber stem end during tuber growing. (4) The tuber stem end at the sprouting stage showed more active gene expression in both starch degradation and resynthesis, suggesting more active export of carbohydrates, than the tuber bud end. The results indicate that the starch accumulation mechanism in the tuber bud end was different between field growing and post-harvest sprouting tubers and that tubers already increased dry matter and average starch granule sizes in the tuber bud end prior to the rapid growth of sprouts.

  7. Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern.

    Science.gov (United States)

    Delaplace, Pierre; Fauconnier, Marie-Laure; Sergeant, Kjell; Dierick, Jean-François; Oufir, Mouhssin; van der Wal, Froukje; America, Antoine H P; Renaut, Jenny; Hausman, Jean-François; du Jardin, Patrick

    2009-01-01

    During post-harvest storage, potato tubers age as they undergo an evolution of their physiological state influencing their sprouting pattern. In the present study, physiological and biochemical approaches were combined to provide new insights on potato (Solanum tuberosum L. cv. Désirée) tuber ageing. An increase in the physiological age index (PAI) value from 0.14 to 0.83 occurred during storage at 4 degrees C over 270 d. Using this reference frame, a proteomic approach was followed based on two-dimensional electrophoresis. In the experimental conditions of this study, a marked proteolysis of patatin occurred after the PAI reached a value of 0.6. In parallel, several glycolytic enzymes were up-regulated and cellular components influencing protein conformation and the response to stress were altered. The equilibrium between the 20S and 26S forms of the proteasome was modified, the 20S form that recycles oxidized proteins being up-regulated. Two proteins belonging to the cytoskeleton were also differentially expressed during ageing. As most of these changes are also observed in an oxidative stress context, an approach focused on antioxidant compounds and enzymes as well as oxidative damage on polyunsaturated fatty acids and proteins was conducted. All the changes observed during ageing seemed to allow the potato tubers to maintain their radical scavenging activity until the end of the storage period as no accumulation of oxidative damage was observed. These data are interpreted considering the impact of reactive oxygen species on the development and the behaviour of other plant systems undergoing ageing or senescence processes.

  8. Extração e isolamento de alfa-solanina de brotos de batata Extraction and isolation of alpha-solanine from potato sprouts

    Directory of Open Access Journals (Sweden)

    Dayse Soave Spoladore

    1983-01-01

    Full Text Available Fresh potato sprouts were extracted with 2% aqueous acetic acid for 48 hours. The crude alkaloidal extract was purified by repeating the operation. Precipitation by concentrated ammonium hydroxide, centrifugation, dissolution of the resulting precipitate in 2% acetic acid, till a clear alkaline supernatant was obtained. The alpha-solanine, obtained by this procedure was purified by successive recristalizations with ethyl alcohol. The total yield was about 0.02% of the fresh material, with purity greater than 99%. IR, ¹H and 13C NMR spectra are shown.

  9. Potato Operation: automatic detection of potato diseases

    Science.gov (United States)

    Lefebvre, Marc; Zimmerman, Thierry; Baur, Charles; Guegerli, Paul; Pun, Thierry

    1995-01-01

    The Potato Operation is a collaborative, multidisciplinary project in the domain of destructive testing of agricultural products. It aims at automatizing pulp sampling of potatoes in order to detect possible viral diseases. Such viruses can decrease fields productivity by a factor of up to ten. A machine, composed of three conveyor belts, a vision system, a robotic arm and controlled by a PC has been built. Potatoes are brought one by one from a bulk to the vision system, where they are seized by a rotating holding device. The sprouts, where the viral activity is maximum, are then detected by an active vision process operating on multiple views. The 3D coordinates of the sampling point are communicated to the robot arm holding a drill. Some flesh is then sampled by the drill, then deposited into an Elisa plate. After sampling, the robot arm washes the drill in order to prevent any contamination. The PC computer simultaneously controls these processes, the conveying of the potatoes, the vision algorithms and the sampling procedure. The master process, that is the vision procedure, makes use of three methods to achieve the sprouts detection. A profile analysis first locates the sprouts as protuberances. Two frontal analyses, respectively based on fluorescence and local variance, confirm the previous detection and provide the 3D coordinate of the sampling zone. The other two processes work by interruption of the master process.

  10. O uso de substâncias inibidoras da brotação de tubérculos de batatinha Use op sprouting inhibiting substances on potato tubers

    Directory of Open Access Journals (Sweden)

    O. J. Boock

    1957-01-01

    Full Text Available A conservação de tubérculos de batatinha para o consumo vem preocupando os pesquisadores em diferentes países, por se tratar de um produto fácilmente perecível. Além dos trabalhos sôbre frigorificação, já bem conhecidos, iniciou-se o emprêgo de substâncias químicas inibidoras da brotação e que impedem as podridões causadas por Fusarium. Dentre essas drogas sobressairam-se o éster metílico do ácido alfanaftaleno acético e o tetracloronitrobenzeno. Os resultados alcançados, embora contraditórios em alguns pontos, vieram demonstrar a viabilidade do seu uso, principalmente em países onde o inverno é rigoroso. Das experiências e observações feitas no Instituto Agronômico de Campinas a partir de 1946, chegou-se à conclusão que as referidas drogas somente trouxeram bons resultados quando os tubérculos tratados foram conservados a baixa temperatura (4°C. Por outro lado, as batatinhas armazenadas a essa temperatura tornaram-se adocicadas e de má aceitação pelos consumidores, devido à formação de açúcares. Mantendo-se as temperaturas entre 18 e 26°C não houve, na maioria dos casos, influência nítida das drogas sôbre a redução da brotação, constatando--se, ao contrário, um aumento na porcentagem de tubérculos deteriorados devido à podridão mole causada por bactérias.Storage of Irish potato tubers under 3 to 5° C, with air moisture remaining between 70 and 90%, affords good results in preventing sprouting of the tubers. As this treatment enables sugar formation in the tubers, the latter should not be used for consumption due to their unpalatability. At temperatures above 8°C sprouting occurs with loss of weight and other disadvantages. In the experiments herein reported two hormonlike substances were used to prevent sprouting under different temperatures. The chemical used were the methyl ester of alphanaphtaleneacetic acid (MENA and 2, 3, 5, 6 tetrachloronitrobenzene (TCNB mixed with talc. Under room

  11. Twenty-six years experience of commercialization on potato irradiation at Shihoro, Japan

    Science.gov (United States)

    Kameyama, K.; Ito, H.

    2000-03-01

    The township of Shihoro is Japan's biggest and most advanced dairy and field farming district which has been producing potatoes as a key crop. The potato irradiation plant was built at Shihoro in 1973 with a potato processing complex and has been shipping some 15,000 tons of sprout-inhibited potatoes a year during the sprouting season. From a technical viewpoint, the radiation efficiency of the Shihoro irradiator is lower than that of the usual irradiation facility using a carton box. The success of the Shihoro irradiator is shown in that radiation processing has been effectively included into one of the storage systems of a huge potato distribution facility. The sprout-inhibited potatoes as such are no guarantee of better business derived from potatoes, but producers see the merit of this process, because it makes possible year-round plant operations and planned shipments, keeps employees in year-round service and prevents rural depopulation, with an increase in the volume of business by expanding the area under crop. Recently in Japan, many companies have interests in commercial irradiation of spices, medical herbs and farm animal feeds before application to common foods.

  12. Twenty-six years experience of commercialization on potato irradiation at Shihoro, Japan

    International Nuclear Information System (INIS)

    Kameyama, K.; Ito, H.

    2000-01-01

    The township of Shihoro is Japan's biggest and most advanced dairy and field farming district which has been producing potatoes as a key crop. The potato irradiation plant was built at Shihoro in 1973 with a potato processing complex and has been shipping some 15,000 tons of sprout-inhibited potatoes a year during the sprouting season. From a technical viewpoint, the radiation efficiency of the Shihoro irradiator is lower than that of the usual irradiation facility using a carton box. The success of the Shihoro irradiator is shown in that radiation processing has been effectively included into one of the storage systems of a huge potato distribution facility. The sprout-inhibited potatoes as such are no guarantee of better business derived from potatoes, but producers see the merit of this process, because it makes possible year-round plant operations and planned shipments, keeps employees in year-round service and prevents rural depopulation, with an increase in the volume of business by expanding the area under crop. Recently in Japan, many companies have interests in commercial irradiation of spices, medical herbs and farm animal feeds before application to common foods. (author)

  13. Role of gibberellins in 1,8-Cineole mediated sprout inhibition

    Science.gov (United States)

    At harvest and for an indeterminate period thereafter, potato tubers are dormant and will not sprout. Tuber dormancy is lost during postharvest storage and the subsequent sprouting results in the loss of tuber processing and nutritional qualities. Therefore, control of tuber sprouting is an essent...

  14. Inibição da brotação de tubérculos de batatinha - Solanum Tuberosum L. Treating irish potato tubers with sprout inhibiting products

    Directory of Open Access Journals (Sweden)

    0. J. Boock

    1959-01-01

    Full Text Available Em prosseguimento aos trabalhos iniciados em 1946, referentes ao uso de substâncias inibidoras da brotação de tubéreulos de batatinha usadas tanto para o consumo como para o plantio, são relatados agora os estudos mais recentes e nos quais foram obtidos resultados dos mais animadores, sobrepujando aquêles já publicados (1. Assim, ficou evidenciado que o produto comercial "Ipnogerm", à base de naftil-acetato de metilo, foi um dos mais eficientes na redução da brotação de tubéreulos de batatinha e na perda de pêso, seguido de "Aaservo", à base de isopropil-fenil-carbamato, enquanto o "Barsprout", à base do éster metflico do ácido alfa-naftaleno acético, foi o que mais impediu o aparecimento de podridão mole, ao contrário do "Aaservo". Em relação à podridão sêca, causada por Fusarium, os produtos mais recomendados foram "Fusarex", à base de tetra-cloro-nitro-benzeno, "Agermina", à base de éster metílico do ácido alfa-naftaleno-acético, e "Ralentone", à base de alcani-metil-naftaleno. De um modo geral, os produtos "Ipnogerm", "Aaservo" e "Tuberite", êste último à base de isopropil-fenil-carbamato, não devem ser empregados para batatas-semente, ao contrário do "Fusarex", "Ralentone" e ''Agermina", fato êste comprovado pelo plantio dos tubérculos tratados.Several sprout inhibiting products were tested on Irish potato tubers. "Ipnogerm", a methyl-acetate compound, gave best results, reducing tuber sprouting and loss of weight, "Aaservo", on the basis of isopropil-phenyl-earbamate, was less effective, while "Barsprout", a methyl ester of the alfa-naphtalene acetic acid, was the most effective on the control of tuber soft rot. The dry rot disease caused by Fusarium, during storage, was better controlled by "Fusarex", a tetra-chloro-nitro-bcnzene product, ''Agermina", a methyl-ester of the alfa-naphtalene acetic acid, and "Ralentone", on the basis of alkani-methyl-naphta-lene. As a rule, "Ipnogerm", "Aaservo", and

  15. The potential of orange-fleshed sweet potato to prevent vitamin A deficiency in Africa.

    Science.gov (United States)

    Gurmu, Fekadu; Hussein, Shimelis; Laing, Mark

    2014-01-01

    Vitamin A deficiency is among major health problems worldwide that leads to blindness, retarded growth and death, particularly in developing countries. In these countries, vitamin A deficiency largely affects pre-school children, pregnant and lactating mothers, and the rural poor. For instance, the predicted prevalence of vitamin A deficiency for 36 sub-Saharan African countries is 19.1%. Different strategies, including vitamin A supplementation, food fortification and dietary diversification, have been used to combat this problem. However, these strategies are not sustainable due to their high costs. Orange-fleshed sweet potato (Ipomoea batatas L. Lam) is a low priced crop, which is part of staple foods in most of sub-Saharan Africa that can be a year-round source of vitamin A. Most of the orange-fleshed sweet potato varieties contain 3000-16000 μg 100 g(-1) of β-carotene and this contributes to 250 to 1300 μg 100 g(-1) Retinol Activity Equivalents (RAE). Therefore, by using orange-fleshed sweet potato, it is possible to improve vitamin A status, increase the bio-availability of different micro-nutrients such as Fe, Zn, Ca and Mg, reduce vitamin A deficiency and hence reduce child mortality rates by 23 to 30%. The article highlights the significance of vitamin A for human nutrition, the effect of vitamin A deficiency, the different prevention methods and the potential of orange- fleshed sweet potato as a food crop to prevent vitamin A deficiency.

  16. Changes in Lipid Peroxidation and Lipolytic and Free-Radical Scavenging Enzyme Activities during Aging and Sprouting of Potato (Solanum tuberosum) Seed-Tubers.

    Science.gov (United States)

    Kumar, GNM.; Knowles, N. R.

    1993-05-01

    Previous research has shown that cell membranes of potato (Solanum tuberosum L. cv Russet Burbank) seed-tubers lose integrity between 7 and 26 months of storage (4[deg]C, 95% relative humidity), and this loss coincides with a significant decrease in growth potential. The age-induced decline in membrane integrity is apparently due to increased peroxidative damage of membrane lipids. Malondialdehyde (MDA) and ethane concentrations (sensitive markers of lipid peroxidation and membrane damage) increased in seed-tuber tissues with advancing age. Moreover, in vivo ethane production from discs of cortex tissue from 13- and 25-month-old seed-tubers was 87% greater (on average) than that from discs from 1-month-old tubers. Calcium suppressed ethane production from all ages of tissue discs, and the effect was concentration dependent. Linoleic acid enhanced ethane production from 5- and 17-month-old tubers by 61 and 228%, respectively, suggesting that older tissue may contain a higher free-radical (FR) titer and/or lower free polyunsaturated fatty acid content. In addition, throughout plant establishment, the internal ethane concentration of older seed-tubers was 54% higher than that of younger seed-tubers. MDA concentration of tuber tissue declined by about 65% during the initial 7 months of storage and then increased 267% as tuber age advanced to 30 months. The age-induced trend in tuber reducing sugar concentration was similar to that of MDA, and the two were linearly correlated. The age-dependent increase in reducing sugars may thus reflect peroxidative degeneration of the amyloplast membrane, leading to increased starch hydrolysis. Compared with 5-month-old seed tubers, 17- and 29-month-old seed-tubers had significantly higher levels of lipofuscin-like fluorescent compounds (FCs), which are produced when MDA reacts with free amino acids. Age-dependent increases in MDA, ethane, and FCs were not associated with higher activities of phospholipase and lipoxygenase in tissue

  17. Study on public acceptance of irradiated potatoes

    International Nuclear Information System (INIS)

    Santa Cruz, S.

    1977-01-01

    4,500 kg potatoes of different sorts were irradiated by a 137 Cs source with a dose of 10 krad. The potatoes were then stored for several months at a room temperature of 6 to 10 0 C and a humidity of 80 to 85%. 0% of the irradiated potatoes sprouted, as compared to 100% of the non-irradiated potatoes. The percentage of rotting varied in the irradiated potatoes, depending on the variety. Cooking tests showed no difference between irradiated and non-irradiated potatoes. The irradiated potatoes were consumed and accepted by about 4,000 persons in the cafeteria of the institute. (AJ) [de

  18. UV-C treatment on physiological response of potato (Solanum tuberosumL.) during low temperature storage.

    Science.gov (United States)

    Lin, Qiong; Xie, Yajing; Liu, Wei; Zhang, Jie; Cheng, Shuzhen; Xie, Xinfang; Guan, Wenqiang; Wang, Zhidong

    2017-01-01

    The storage of potato tuber ( Solanum tuberosum L.) at low temperatures minimizes sprouting and disease but can cause cold-induced sweetening (CIS), which leads to the production of the cancerogenic substance acrylamide during the frying processing. The aim of this research was to investigate the effects of UV-C treatment on CIS in cold stored potato tuber. 'Atlantic' potatoes were treated with UV-C for an hour and then stored at 4 °C up to 28 days. The UV-C treatment significantly prevented the increase of malondialdehyde content (an indicator of the prevention of oxidative injury) in potato cells during storage. The accumulation of reducing sugars, particularly fructose and glucose, was significantly reduced by UV-C treatment possibly due to the regulation of the gene cascade, sucrose phosphate synthase , invertase inhibitor 1/3 , and invertase 1 in potato tuber, which were observed to be differently expressed between treated and untreated potatoes during low temperature storage. In summary, UV-C treatment prevented the existence of oxidative injury in potato cells, thus, lowered the amount of reducing sugar accumulation during low temperature storage of potato tubers.

  19. Potato plant poisoning - green tubers and sprouts

    Science.gov (United States)

    ... large ingestions. These poisonings can be very dangerous. Symptoms may include: Vomiting Stomach or abdominal pain Diarrhea Fever Delirium Dilated pupils Hallucinations Headache Loss of sensation Lower ...

  20. Preservation of potatoes by gamma irradiation

    International Nuclear Information System (INIS)

    Nouani, A.; Boussaha, A.

    1987-01-01

    In Algeria, potatoes are a major food item in nutrition habits. Because of lack of cold storage facilities, losses can reach up to 40% of the total output of summer harvest. This paper describes the first experiments on the application of gamma irradiation for the preservation of local varieties of potatoes. Losses are strongly reduced by inhibition sprouting effect of irradiation and reduction of sugars content has no significant influence on the acceptability of irradiated potatoes

  1. assessment of six storage methods for seed potato storage in nigeria

    African Journals Online (AJOL)

    ... sprout number and length per tuber at the end of the storage were also recorded. Results showed that only polythene bag reduced tuber weight loss, but resulted in highest tuber rots due to soft rot disease of potato. Poor light condition in jute bags encouraged the development of long and etiolated sprouts of seed potato.

  2. Sprout inhibition in roots, tubers and bulbs

    International Nuclear Information System (INIS)

    Luna C, P.C.

    1992-05-01

    The treatment with ionizing radiations to low dose impedes that appear sprouts in the tubers (potatoes); bulbs (onion and garlic) and in roots like the ginger and the yucca. The purpose is to inhibit the germination during the process of manipulation and storage, and this way to avoid the lost ones post crop of these products. The radiation dose required to inhibit the germination goes to depend of: the development conditions, the differences of variety, of the storage state of the bulbs and the conditions of cured and storage. (Author)

  3. Impact of post-harvest radiation treatment timing on shelf life and quality characteristics of potatoes.

    Science.gov (United States)

    Rezaee, Mehdi; Almassi, Morteza; Minaei, Saeed; Paknejad, Farzad

    2013-04-01

    The effects of gamma radiation treatment (50 and 100 Gy) on potato tubers irradiated at different days (10th, 30th and 50th) after harvest were studied during 5 months of storage at 10°C using Agria and Marfona varieties. A factorial experiment was done, based on a Randomized Complete Block Design with four replications. The 100 Gy radiation treatments on 10th and 30th days after harvest completely prevented sprouting at both varieties studied but on 50th day only Agria tubers not sprouted. This study indicated that early irradiation and absorbed radiation doses significantly decreased sprouting, percent weight loss and specific gravity of tubers. Reducing sugar content significantly increased by delay in irradiation and lower dose of radiation while non-reducing sugars did not decrease significantly by delay in irradiation and higher dose of radiation. The least increase in reducing sugars (10.2%) and most decrease in non-reducing sugar (-12.75%) were observed in tubers that irradiated on 10th day after harvest. The content of ascorbic acid was decreased by irradiation with higher dose. Although delay in irradiation caused less loss of ascorbic acid (8.5%) but showed greater metabolic changes as sprouting, weight loss, firmness, and sugars contents. Also, more increased delay in irradiation needed higher radiation doses for sprout inhibition.

  4. Impact of post-harvest radiation treatment timing on shelf life and quality characteristics of potatoes

    International Nuclear Information System (INIS)

    Rezaee, Mehdi; Paknejad, Farzad; Almassi, Morteza; Minaei, Saeed

    2013-01-01

    The effects of gamma radiation treatment (50 and 100 Gy) on potato tubers irradiated at different days (10th, 30th and 50th) after harvest were studied during 5 months of storage at 10°C using Agria and Marfona varieties. A factorial experiment was done, based on a Randomized Complete Block Design with four replications. The 100 Gy radiation treatments on 10th and 30th days after harvest completely prevented sprouting at both varieties studied but on 50th day only Agria tubers did not sprout. This study indicated that early irradiation and absorbed radiation doses significantly decreased sprouting, percent weight loss and specific gravity of tubers. Reducing sugar content significantly increased by delay in irradiation and lower dose of radiation while non-reducing sugars did not decrease significantly by delay in irradiation and higher dose of radiation. The least increase in reducing sugars (10.2%) and most decrease in non-reducing sugar (-12.75%) were observed in tubers that irradiated on 10th day after harvest. The content of ascorbic acid was decreased by irradiation with higher dose. Although delay in irradiation caused less loss of ascorbic acid (8.5%) but showed greater metabolic changes as sprouting, weight loss, firmness, and sugars contents. Also, more increased delay in irradiation needed higher radiation doses for sprout inhibition. (author)

  5. Dietary Intake of Sulforaphane-Rich Broccoli Sprout Extracts during Juvenile and Adolescence Can Prevent Phencyclidine-Induced Cognitive Deficits at Adulthood.

    Directory of Open Access Journals (Sweden)

    Yumi Shirai

    Full Text Available Oxidative stress and inflammation play a role in cognitive impairment, which is a core symptom of schizophrenia. Furthermore, a hallmark of the pathophysiology of this disease is the dysfunction of cortical inhibitory γ-aminobutyric acid (GABA neurons expressing parvalbumin (PV, which is also involved in cognitive impairment. Sulforaphane (SFN, an isothiocyanate derived from broccoli, is a potent activator of the transcription factor Nrf2, which plays a central role in the inducible expressions of many cytoprotective genes in response to oxidative stress. Keap1 is a cytoplasmic protein that is essential for the regulation of Nrf2 activity. Here, we found that pretreatment with SFN attenuated cognitive deficits, the increase in 8-oxo-dG-positive cells, and the decrease in PV-positive cells in the medial prefrontal cortex and hippocampus after repeated administration of phencyclidine (PCP. Furthermore, PCP-induced cognitive deficits were improved by the subsequent subchronic administration of SFN. Interestingly, the dietary intake of glucoraphanin (a glucosinolate precursor of SFN during the juvenile and adolescence prevented the onset of PCP-induced cognitive deficits as well as the increase in 8-oxo-dG-positive cells and the decrease in PV-positive cells in the brain at adulthood. Moreover, the NRF2 gene and the KEAP1 gene had an epistatic effect on cognitive impairment (e.g., working memory and processing speed in patients with schizophrenia. These findings suggest that SFN may have prophylactic and therapeutic effects on cognitive impairment in schizophrenia. Therefore, the dietary intake of SFN-rich broccoli sprouts during the juvenile and adolescence may prevent the onset of psychosis at adulthood.

  6. Cancer-preventive Properties of an Anthocyanin-enriched Sweet Potato in the APCMIN Mouse Model.

    Science.gov (United States)

    Asadi, Khalid; Ferguson, Lynnette R; Philpott, Martin; Karunasinghe, Nishi

    2017-09-01

    Anthocyanin-rich foods and preparations have been reported to reduce the risk of life-style related diseases, including cancer. The SL222 sweet potato, a purple-fleshed cultivar developed in New Zealand, accumulates high levels of anthocyanins in its storage root. We examined the chemopreventative properties of the SL222 sweet potato in the C57BL/6J-APC MIN/+ (APC MIN ) mouse, a genetic model of colorectal cancer. APC MIN and C57BL/6J wild-type mice (n=160) were divided into four feeding groups consuming diets containing 10% SL222 sweet potato flesh, 10% SL222 sweet potato skin, or 0.12% ARE (Anthocyanin rich-extract prepared from SL222 sweet potato at a concentration equivalent to the flesh-supplemented diet) or a control diet (AIN-76A) for 18 weeks. At 120 days of age, the mice were anaesthetised, and blood samples were collected before the mice were sacrificed. The intestines were used for adenoma enumeration. The SL222 sweet potato-supplemented diets reduced the adenoma number in the APC MIN mice. These data have significant implications for the use of this sweet potato variant in protection against colorectal cancer.

  7. 3-Methyl-1-phenyl-2-pyrazolin-5-one or N-acetylcysteine prevents hippocampal mossy fiber sprouting and rectifies subsequent convulsive susceptibility in a rat model of kainic acid-induced seizure ceased by pentobarbital.

    Science.gov (United States)

    Nomura, Shohei; Shimakawa, Shuichi; Miyamoto, Ryohei; Fukui, Miho; Tamai, Hiroshi

    2014-11-24

    There is accumulating evidence that reactive oxygen species are involved in the development of seizures under pathological conditions, and antioxidant treatments are a novel therapeutic approach for epilepsy. The kainic acid (KA) model of induced seizures has been widely used to study temporal lobe epilepsy. However, research on the use of free radical scavengers following KA-induced status epilepticus (SE) is limited. We examined whether antioxidants already used in humans could reduce hippocampal neuronal cell loss, mossy fiber sprouting and the acquisition of hyperexcitability when administered as a single dose after SE. The antioxidant 3-methyl-1-phenyl-2-pyrazolin-5-one (edaravone) (30mg/kg) or N-acetylcysteine (NAC) (30mg/kg) was administered after KA-induced SE ceased by pentobarbital. We evaluated neuronal cell viability 1 week after SE, determined the threshold for seizures induced by inhalation of flurothyl ether 12 weeks after SE, and examined the extent of mossy fiber sprouting 12 weeks after SE. We found that edaravone or NAC prevented neuronal cell loss and mossy fiber sprouting, and increased the threshold for seizures induced by flurothyl ether, even when administered after KA-induced SE. These results demonstrate that a single dose of edaravone or NAC can protect against neuronal cell loss and epileptogenesis when administered after SE ceased by pentobarbital. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Some Results on Sprout

    DEFF Research Database (Denmark)

    Banik, Subhadeep

    2015-01-01

    Sprout is a lightweight stream cipher proposed by Armknecht and Mikhalev at FSE 2015. It has a Grain-like structure with two state Registers of size 40 bits each, which is exactly half the state size of Grain v1. In spite of this, the cipher does not appear to lose in security against generic Time...... secret key, there exist around 230 IVs for which the LFSR used in Sprout enters the all zero state during the keystream generating phase. Using this observation, we will first show that it is possible to enumerate Key-IV pairs that produce keystream bits with period as small as 80. We will then outline...

  9. Irradiation processing of potatoes, onions and garlic

    International Nuclear Information System (INIS)

    Jan, M.; Starr, A.; Wahid, M.; Ahmad, A.; Khan, I.

    1992-07-01

    In this report, studies have been conducted on irradiation preservation of potatoes, onions and garlic involving different varieties, harvesting season and storage life. As a result of these investigations, optimum conditions were established for each crop. The effect of irradiation and storage on sprouting, rottage and weight loss of potatoes is presented. The sprouting initiated in the unirradiated samples after 2 months reaching 51, 80 and 100% on 4, 5 and 6 months storage respectively. Weight losses were observed in the irradiated and unirradiated samples. (A.B.)

  10. Prevalence of Six Viruses in Potato Seed Tubers Produced in ...

    African Journals Online (AJOL)

    Potato (Solanum tuberosum L.) is an important cash-food crop, which is widely grown in three of the five agro-ecological zones of Cameroon. A study was carried out to determine the prevalence of PVA, PLRV, PVM, PVS, PVX and PVY in 1175 sprouted potato seed tubers of different diameters collected from four seed ...

  11. Biological control of Rhizoctonia solani on potato by Verticillium ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-06-03

    Jun 3, 2009 ... sclerotia of R. solani was reduced by V. biguttatum isolates. V. biguttatum also significantly reduced the disease severity of R. solani on potato sprouts in pot experiments. This is the first report of V. biguttatum from sclerotia of R. solani in Turkey. Key words: Bio-control, potato, Rhizoctonia solani, Verticillium ...

  12. Storage performance of Taiwanese sweet potato cultivars.

    Science.gov (United States)

    Huang, Che-Lun; Liao, Wayne C; Chan, Chin-Feng; Lai, Yung-Chang

    2014-12-01

    Three sweet potato cultivars (TNG57, TNG66, and TNG73), provided by the Taiwanese Agricultural Research Institute (TARI), were stored at either 15 °C or under ambient conditions (23.8 ~ 28.4 °C and 77.1 ~ 81.0 % of relative humidity). Sweet potato roots were randomly chosen from each replicate and evaluated for measurement of weight loss, sugar content analysis, and sprouting after 0, 14, 24, 48, 56, 70, 84, and 98 days of storage. Fresh sweet potato roots were baked at 200 °C for 60 min then samples were taken for sugar analysis. After 14 days of ambient condition storage, the sprouting percentages for TNG57, TNG66, and TNG73 were 100, 85, and 95 % respectively. When sweet potatoes were stored at 15 °C, the weight loss became less and no sweet potato root sprouted after 14 days of storage. Because manufacturers can store sweet potatoes at 15 °C for almost 2 month without other treatments, the supply capacity shortage in July and September can be reduced. The total sugar content slowly increased along with increasing the storage time. After baking, the total sugar content of sweet potatoes significantly increased due to the formation of maltose. Maltose became the major sugar of baked sweet potatoes. Raw sweet potatoes stored at 15 °C had higher total sugar contents after baking than those stored under ambient conditions. Raw sweet potatoes were recommended to be stored at 15 °C before baking.

  13. Study of the techno-economic feasibilities of potato and onion irradiation in Syria

    International Nuclear Information System (INIS)

    Al-Bachir, M.; Sharabi, N.; Midani, M.A.

    1991-06-01

    Ionizing radiation has been used for food preservation in many ways, among them sprout inhibition in onion and potato. Experiments showed that irradiation of these crops using doses between 50 - 150 Gy prevented sprouting for up to 6 months during storage. Priliminary experiments in Syria proved the effectiveness of such treatment in these two crops. In addition to the technical, social, and political feasibility, this study includes, general description of the suggested plant ( 60 Co 3.7 PBq), estimated cost, location, and the tentative operation schedual. The study showed that the cost of irradiation will be about $ 8.7 per ton, this will save the country about $ 3.760.000 per year. The study showed the possitive effect of establishing this plant. (author). 74 refs., 44 tabs., 15 figs

  14. Transient decreases in methylation at 5'-cCGG-3' sequences in potato (Solanum tuberosum L.) meristem DNA during progression of tubers through dormancy precede the resumption of sprout growth.

    Science.gov (United States)

    Law, R David; Suttle, Jeffrey C

    2003-02-01

    The 5-methylcytosine (5mC) content in DNA of tuber meristems isolated from field-grown potatoes (Solanum tuberosum L.) was determined during a 7-month storage period at 3 degrees C for three growing/postharvest seasons. No significant changes in 5mC levels were noted genome-wide or within 5'-CG-3' dinucleotide sequences, 5'-CG-3' islands or 5'-CA(T)G-3' trinucleotide sequences during storage. However, a consistent but transient 50-70% decrease in methylation at both cytosines within 5'-CCGG-3' sequences was detected that peaked 112-194 days after harvest. This result was corroborated by methylation-sensitive amplified fragment length polymorphism analysis of meristem DNA. Similar to tuber meristems undergoing progression through natural dormancy, premature chemical termination of dormancy resulted in rapid, transient 5'-CCGG-3' demethylation in meristem DNA. Minimum methylation levels at this sequence preceded initiation of high levels of de novo DNA synthesis by two days. Cytosine methylation status was also followed in in vitro-generated potato microtubers during 7 months of post-harvest storage. As in DNA from tuber bud meristems, no changes in genome-wide 5mC content or methylation at 5'-CA(T)G-3' or 5'-CG-3' island sequences were noted in microtuber DNA. However, there was a transient 46% drop in methylation at 5'-CG-3' dinucleotides concomitant with minimum levels of 5'-CCGG-3' methylation (30-60% below those in dormant microtubers) 57-98 days after harvest. As microtubers exited dormancy, there were sustained three- and seven-fold increases in RNA and DNA synthesis rates, peaking on or after 98 days of storage, respectively. Together, these data demonstrate that demethylation of 5'-CCGG-3' sequences occurs independently of tuber age during dormancy progression and precedes transcriptional activation of genes leading to cell division and meristem growth in potatoes.

  15. physiological mechanisms for potato dormancy release

    African Journals Online (AJOL)

    ACSS

    time bear significant economic importance, this review considers the regulation of dormancy and sprouting of potato by phytohormones and reactive ... practical purposes, and is defined as the period from dehaulming to the time when 80% ..... in endogenous abscisic acid (ABA) content and in ABA-regulated transcripts and ...

  16. Purple sweet potato (Ipomoea batatas L.) anthocyanins: preventive effect on acute and subacute alcoholic liver damage and dealcoholic effect.

    Science.gov (United States)

    Sun, Hongnan; Mu, Taihua; Liu, Xingli; Zhang, Miao; Chen, Jingwang

    2014-03-19

    This study aimed to investigate the dealcoholic effect and preventive effect of anthocyanins from purple sweet potato (PSPAs) on acute and subacute alcoholic liver damage (ALD). Seven-week-old male inbred mice were grouped into five groups: control group (without PSPAs and ethanol treatments), model group (with ethanol treatment only), low-dose group (50 mg PSPAs/kg body weight), middle-dose group (125 mg PSPAs/kg body weight), and high-dose group (375 mg PSPAs/kg body weight), and the mice in all groups were administered intragastrically. Biochemical parameters of serum and liver were determined, and the histopathological changes of liver tissue were also analyzed. Results showed that all tested parameters were ameliorated after consumption of PSPAs. Therefore, PSPAs have preventive effect on acute and subacute ALD. It is suggested that PSPAs could be used as a supplementary reagent during prophylactic and curative managements of ALD.

  17. Effect of gamma irradiated parenchyma on the growth of irradiated potato tuber buds

    International Nuclear Information System (INIS)

    Fernandez Gonzalez, J.; Garcia Collantes, M. A.

    1976-01-01

    The development of buds greffed on irradiated potato parenchyma was studied. The irradiated parenchyma does not influence the sprouting capacity of buds, but it affects the way they develop. (Author) 9 refs

  18. Biochemical properties and expression analysis of potato cytokinin oxidases during tuber dormancy

    Science.gov (United States)

    At harvest and for an indeterminate period thereafter, potato tubers are dormant and will not sprout. Tuber dormancy is lost during postharvest storage and the subsequent sprouting results in the loss of tuber processing and nutritional qualities. Endogenous cytokinins have been posited to play a p...

  19. Control of Rhizoctonia solani in potato by biological, chemical and integrated measures

    NARCIS (Netherlands)

    Jager, G.; Velvis, H.; Lamers, J.G.; Mulder, A.; Roosjen, J.

    1991-01-01

    The effects of biological, chemical and integrated control on the formation of selerotia ofRhizoctonia solani on new potato tubers were studied in experimental fields. Sprouts of seed tubers, sprouted in daylight, were inoculated withVerticillium biguttatum, an ecologically obligate mycoparasite

  20. Comparative storage studies of selected varieties of potatoes treated with CIPC and gamma radiation

    International Nuclear Information System (INIS)

    Islam, M.S.; Karim, A.; Hossain, M.M.; Hossain, A.

    1990-01-01

    Three varieties of potatoes (Diamant, Kufri sinduri and Lal pakri) were treated with 8 Krad of gamma radiation and CIPC at the rate of 2 gm/kg of potatoes and were stored at 4 deg C, 15 deg. C and ambient temperature. The control, irradiated and CIPC treated potatoes at ambient temperature spoiled within 2 months of storage. Control potatoes stored at 15 deg. C lost their market value due to sprouting and shrinkage after 6 months of storage. Irradiated potatoes stored at 15 deg. C lost weight at a slower rate than CIPC treated samples stored at the same temperature and control potatoes stored at 4 deg. C. All the potatoes in control samples sprouted within 3 months of storage at 15 deg. C whereas no sprouting was recorded in any variety of potatoes treated with radiation or CIPC and stored at 15 deg. C for 6 months. After 6 months of storage reducing sugar content was much lower in the irradiated or CIPC treated potatoes than the control potatoes stored at 4 deg. C. The irradiated tubers stored at 15 deg. C and control tubers stored at 4 deg. C retained excellent physical appearance and market quality at the end of 6 months storage though irradiated potatoes were more attractive

  1. Healthy food trends -- brussels sprouts

    Science.gov (United States)

    ... or yellowing. Put Brussels sprouts on your weekly shopping list. They will last in the refrigerator for ... the first to achieve this important distinction for online health information and services. Learn more about A. ...

  2. In vivo thermoterapy: attempt to eliminate virus in potato tuber

    Science.gov (United States)

    Ayu Astarini, Ida; Margareth, Deborah; Temaja, I. Gede Rai Maya

    2018-03-01

    Potato is one of an important vegetable crop in Indonesia, including Bali. Main potato production areas in Bali are at Bedugul region, 1.200 m above sea level. Potato production in Bali continued to decrease due to diseases infection, such as early blight, late blight, black leg and virus diseases. Potato farmers in Bali usually set aside their harvest as seed potatoes, resulting in virus diseases being carried out on the next planting seasons and eventually would decrease potato production both in quantity and quality. Four types of virus were confirmed: PVY, PVX, PVS and PRLV. A number of studies have reported thermotherapy technique has been employed to eliminate potato virus in vitro. However, this technique is not readily available for farmers, since there is no established tissue culture laboratory to support. Therefore, there is an urgent need to develop a more practical method. The objective of this study was to eliminate virus on seed potatoes using thermotherapy on tuber. Seed potatoes with 1 cm sprout which were virus positive were placed on sterile charred rice paddy husk, and then put into a humidified incubator. Tubers were exposed to 37°C for four days followed by 34°C for three days alternately for two weeks and three weeks duration. Four tubers received heat exposure regime for each virus type. After thermotherapy, potato tubers were transferred to pots containing charred rice paddy husk and maintain for three weeks until new leaves emerge for virus analyses. Results show that seed tubers experienced delayed growth after thermotherapy. Control plants sprout one week after thermotherapy, while treated plants were not yet sprouting. Experiment is currently underway. It is expected that heat treatment on tuber will give a practical method for farmers to eliminate virus of seed potatoes.

  3. Sprout Singling in North Alabama Hardwoods

    Science.gov (United States)

    Kozma Naka; Philip G. Cannon

    2004-01-01

    Many commercial hardwood species grow quite well in northern Alabama and most regenerate by stump sprouts after harvest. The number of sprouts on a stump depends on several factors such as species and stump size. To determine if the practice of singling (removing all but the single best sprout from a stump) might be a means of accelerating the growth rate of one stem...

  4. Microbiological safety evaluations and recommendations on sprouted seeds. National Advisory Committee on Microbiological Criteria for Foods.

    Science.gov (United States)

    1999-11-15

    In 1997, the National Advisory Committee on Microbiological Criteria for Foods (NACMCF/the Committee) was asked to review the current literature on sprout-associated outbreaks: identify the organisms and production practices of greatest public health concern: prioritize research needs: and provide recommendations on intervention and prevention strategies. In response to this charge, the Fresh Produce Work Group (FPWG) documented the relevant epidemiology and microbial ecology of sprout-associated outbreaks and reviewed current industry practices and initiatives related to the growing of seed and the production of sprouts. Sprouts have been identified as a special problem because of the potential for pathogen growth during the sprouting process. If pathogens are present on or in the seed, sprouting conditions may favor their proliferation. There is no inherent step in the production of raw sprouts to reduce or eliminate pathogens. Contaminated seed is the likely source for most reported sprout-associated outbreaks. Research has been initiated on methods to reduce or eliminate pathogenic bacteria on seeds and sprouts and some treatments show promise. However, to date, no single treatment has been shown to completely eliminate pathogens under experimental conditions used. Finally, the Committee found that, at the time of the charge, there was a lack of fundamental food safety knowledge along the continuum from seed production through sprout consumption. More recently, many have become aware of the potential for this food to be a vehicle for foodborne illness and the need for appropriate controls: however, such awareness is not universal. Although seed appears to be the most likely source of contamination in sprout associated outbreaks, practices and conditions at the sprouting facility may also impact on the safety of the finished product. In recent sprout-associated outbreak investigations, facilities associated with outbreaks did not consistently apply seed

  5. Control of sweet potato virus diseases.

    Science.gov (United States)

    Loebenstein, Gad

    2015-01-01

    Sweet potato (Ipomoea batatas) is ranked seventh in global food crop production and is the third most important root crop after potato and cassava. Sweet potatoes are vegetative propagated from vines, root slips (sprouts), or tubers. Therefore, virus diseases can be a major constrain, reducing yields markedly, often more than 50%. The main viruses worldwide are Sweet potato feathery mottle virus (SPFMV) and Sweet potato chlorotic stunt virus (SPCSV). Effects on yields by SPFMV or SPCSV alone are minor, or but in complex infection by the two or other viruses yield losses of 50%. The orthodox way of controlling viruses in vegetative propagated crops is by supplying the growers with virus-tested planting material. High-yielding plants are tested for freedom of viruses by PCR, serology, and grafting to sweet potato virus indicator plants. After this, meristem tips are taken from those plants that reacted negative. The meristems were grown into plants which were kept under insect-proof conditions and away from other sweet potato material for distribution to farmers after another cycle of reproduction. © 2015 Elsevier Inc. All rights reserved.

  6. Virus free seed potato production through sprout cutting technique ...

    African Journals Online (AJOL)

    ONOS

    2010-09-06

    Sep 6, 2010 ... strains and studies on losses in commercial varieties. Ann. Sci. Rept. CPR). Simla, India, pp. 88-89. Sreenivasulu P, Naidu RA, Nayudu MV (1989). Physiology of virus infected plants. South Asian Publishers, New Delhi, India, p. 164. Venhocks JL (1965). Photosynthesis and carbohydrate metabolism of.

  7. Future problems on the storage of potatoes, (2)

    International Nuclear Information System (INIS)

    Sato, Masato

    1981-01-01

    The effects of different storage methods on the composition, and the properties of potatoes and their suitability for processing were examined using 3 early and 3 late cultivars. When potatoes were stored at room temperature, although the results varied slightly with the cultivars, there was from 3 to 6% decrease in weight by the next spring, the content of starch decreased strikingly from the beginning of storage to around the end of February, the content of total free sugars increased rapidly from around December to the end of February, the amino acid content tended to increase during the storage period, and 65% of the total ascorbic acid in potatoes still remained early in April. Potatoes lost their shape during cooking more easily as they were stored longer. When potatoes were stored at constant temperature of 5 deg C, the decrease in weight occurred more slowly than that at 2 or 10 deg C. As the storage temperature was lower, the amount of both reduced and non-reduced sugars accumulated more, which make fried potatoes brownish. The potatoes stored at 10 deg C had the best color when they were fried, but began to sprout in mid-December. Thus, to ensure long-term supply for making good fried potatoes, potatoes should be stored at 5 deg C, then be kept at 20 - 25 deg C for 1 - 2 weeks to reduce the sugar content before processing. The sprouting of potatoes was inhibited prominently when MH-30 was given during the intermediate stage of tuber enlargement. The potatoes 40 days after harvest, irradiated with 7 or 15 krad and stored at normal temperature lost less weight than those which were not irradiated. (Kaihara, S.)

  8. Effect of wound healing period and temperature, irradiation and post-irradiation storage temperature on the rot incidence of potatoes, after infection with Fasurium sulfurium

    NARCIS (Netherlands)

    Langerak, D.I.; Wolters, T.C.; Quan, V.H.; Oularbi, S.; Tayeb, Y.; Vroomen, L.H.M.

    1988-01-01

    Losses during star age in potatoes are mainly due to sprouting and rotting. It has indicated that irradiation by low dose (50 to 100 Gy) during the dormancy period is most e ffective for sprout inhibition. Some investigators, however , stated an increase of storage rot after an irradiation

  9. Radiation preservation of foods of plant origin. Part 1. Potatoes and other tuber crops

    International Nuclear Information System (INIS)

    Thomas, P.

    1984-01-01

    In Part 1 of a planned series of articles on preservation of foods of plant origin by gamma irradiation, the current state of research on the technological, nutritional, and biochemical aspects of sprout inhibition of potatoes and other tuber crops are reviewed. These include varietal responses, dose effects, time of irradiation, pre- and postirradiation storage, and handling requirements; postirradiation changes in carbohydrates, ascorbic acid, amino acids, and other nutrients; respiration; biochemical mechanisms involved in sprout inhibition; wound healing and microbial infection during storage; formation of wound and light-induced glycoalkaloids and identification of irradiated potatoes. The culinary and processing qualities with particular reference to darkening of boiled and processed potatoes are discussed. The prospects of irradiation on an industrial scale as an alternative to chemical sprout inhibitors or mechanical refrigeration are considered

  10. Effects of gamma radiation on the physical properties of some South African varieties of potatoes

    International Nuclear Information System (INIS)

    Winchester, R.V.

    1975-01-01

    Effects of gamma irradiation on the mass loss, specific gravity, firmness, sprouting, and rotting of five varieties of potatoes grown in South Africa have been studied. Doses of up to 15 krad inhibit sprouting without detrimental effects on other physical properties, and the culinary properties are expected to be unaffected. Excessive mass loss, shrinkage, and rotting found in some varieties are ascribed to unsuitable storage conditions rather than to irradiation. (orig.) [de

  11. Substancias con actividad citoquinínica estimulan la brotación de yemas en tubérculos de papa Substâncias com atividade citocinínica estimulam a brotação de gemas em tubérculos de batata Cytokinin-like substences activity promotes shoot sprouting in potato tubers

    Directory of Open Access Journals (Sweden)

    Manuel García-Flórez

    2009-09-01

    ção coordenada de vários fitorreguladores. A presença de citocininas em S. phureja Juz. et Buk. var. Criolla Colombia e em S. tuberosum L. variedades ICA-Única e Tuquerreña foi determinada em diferentes estádios do desenvolvimento do tubérculo: 00 - tubérculo em estádio de dormência; 03 - tubérculo no final da dormência e brotos com 2 a 3 mm de comprimento; e 40 - início da tuberização, espessamento das extremidades dos estolões ao dobro do diâmetro inicial. As citocininas foram extraídas por cromatografia de camada delgada e sua detecção e quantificação observadas por meio do bioensaio da massa cotiledonar de rabanete (Raphanus sativus. Na espécie precoce S. phureja Juz. et Buk. var. Criolla Colombia a presença de citocininas foi detectada em quantidades significativas nos estádios 00 e 03. Por outro lado, nas variedades de S. tuberosum L. as citocininas foram significativamente maiores no estádio 03 para 'ICA-Única' (variedade precoce, e a sua presença não foi detectada na var. Tuquerreña (variedade tardia. Demonstrou-se a relação direta entre a concentração de citocininas e a precocidade das variedades analisadas durante a tuberização: as citocininas estiveram presentes nas variedades precoces e ausentes na tardia.Before sprouting, potato tubers pass through a period of dormancy which has an specific time for each variety. When the conditions are suitable for tuberset, stolon elongation stops and swelling starts between two last nodes of each stolon. Many factors are involved in this process, but an important and coordinated action between hormones is proposed. Cytokinins levels in both S. tuberosum L. varieties ICA-Única and Tuquerreña and S. phureja Juz. et Buk. var. Criolla Colombia were quantified during different stages of tuber development: 00 - potato tuber in stage of dormancy; 03 - tubers in break dormancy with buds between 2 to 3 mm of length; and 40 - initiation of tuber formation; swelling and duplicating initial diameter

  12. Economic feasibility study of onion and potato irradiation in the Syrian Arab Republic

    International Nuclear Information System (INIS)

    Al-Bachir, M.; El-Den-Sharabi, N.; Midani, M.Ayman

    1995-01-01

    The purpose of this study was to examine the economic and financial feasibility of a food irradiation project proposed by the Syrian Atomic Energy Commission. The prime purpose of the project was to irradiate two important agricultural products, namely, onion and potato. The irradiation process has the effect of reducing sprouting significantly. In Syria onions are usually stored in well ventilated warehouses at ambient temperature. Our investigation showed that sprouting started after 1.5 months of storage. It is estimated that the sprouting rate reaches 100% after five months of storage. This implies a waste factor of 50%. (author). 7 refs., 4 tabs

  13. Effect of gamma irradiated parenchyma on the growth of irradiated potato tuber buds; Efecto del parenquina irradiado sobre el desarrollo de las yemas de tuberculos de patata tratados por radiacion GAMMA

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez Gonzalez, J.; Garcia Collantes, M. A.

    1976-07-01

    The development of buds greffed on irradiated potato parenchyma was studied. The irradiated parenchyma does not influence the sprouting capacity of buds, but it affects the way they develop. (Author) 9 refs.

  14. Evaluating the impact of sprouting conditions on the glucosinolate content of Brassica oleracea sprouts.

    Science.gov (United States)

    Vale, A P; Santos, J; Brito, N V; Fernandes, D; Rosa, E; Oliveira, M Beatriz P P

    2015-07-01

    The glucosinolates content of brassica plants is a distinctive characteristic, representing a healthy advantage as many of these compounds are associated to antioxidant and anti-carcinogenic properties. Brassica sprouts are still an underutilized source of these bioactive compounds. In this work, four varieties of brassica sprouts (red cabbage, broccoli, Galega kale and Penca cabbage), including two local varieties from the North of Portugal, were grown to evaluate the glucosinolate profile and myrosinase activity during the sprouting. Also the influence of light/darkness exposure during sprouting on the glucosinolate content was assessed. Glucosinolate content and myrosinase activity of the sprouts was evaluated by HPLC methods. All sprouts revealed a higher content of aliphatic glucosinolates than of indole glucosinolates, contrary to the profile described for most of brassica mature plants. Galega kale sprouts had the highest glucosinolate content, mainly sinigrin and glucoiberin, which are recognized for their beneficial health effects. Penca cabbage sprouts were particularly richer in glucoraphanin, who was also one of the major compounds in broccoli sprouts. Red cabbage showed a higher content of progoitrin. Regarding myrosinase activity, Galega kale sprouts showed the highest values, revealing that the use of light/dark cycles and a sprouting phase of 7-9 days could be beneficial to preserve the glucosinolate content of this variety. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Impact of selenium enrichment on seed potato tubers

    Directory of Open Access Journals (Sweden)

    M. TURAKAINEN

    2008-12-01

    Full Text Available The aim of this study was to investigate the effect of Se enrichment on the growth of sprouts and growth vigour of seed potatoes (Solanum tuberosum L. stored for 2 to 8 months. Our results showed that Se did not affect the duration of dormancy. At the high addition levels (0.075 and 0.9 mg kg-1 quartz sand, Se had some positive effects on the growth of sprouts. The peak sprouting capacity was reached after 8 months of storage. The highest Se enrichment of tubers had some positive effect on the free putrescine content in sprouts. However, the better growth of sprouts was not consistent with the growth vigour of the seed tubers and yield produced. Selenium had no significant effect on the malondialdehyde (MDA or on the concentration of soluble sugars and starch. No significant effect of added Se on the early growth, stem and tuber numbers and yield parameters was observed. Irrespective of the level of Se added, the highest yield was harvested from plants produced with seed tubers stored for 6 months. Our results indicate that Se had some positive effects on the growth of sprouts, but it had no consistent effect on the growth vigour of seed tubers.;

  16. Temperature-dependent regulation of sugar metabolism in wild-type and low-invertase transgenic chipping potatoes during and after cooling for low-temperature storage

    Science.gov (United States)

    Regulation of sugar metabolism in cold-stored potato tubers has significant ramifications for potato chip and French fry producers and consumers. Though low-temperature storage reduces losses due to sprouting and disease, it induces accumulation of the reducing sugars glucose and fructose. These rea...

  17. Long-term storage of irradiated potatoes for use in processed products, (1)

    International Nuclear Information System (INIS)

    Aoki, Shohei; Umeda, Keiji; Kameyama, Kenji.

    1982-01-01

    All potatoes irradiated at the early period of dormancy and stored at 10 0 C before and after irradiation showed a reducing sugar content of less than 3 mg/g and are suitable for processing after 6 months of post harvest storage. However, the disadvantage was the shrinkage effect on the potatoes during the long-term of storage at 10 0 C. The reducing sugar content of potatoes irradiated at the last period of dormancy and stored at 10 0 C could be decreased rapidly to an acceptable level for processing by reconditioning at 15 - 18 0 C, RH 80% for 2 - 3 weeks. The sprouts existing at the last period of dormancy were few and they dried up and dropped off following irradiation. Occasionally new buds of unusual form appeared after irradiation during the 6 months storage, but these thin sprouts had no adverse effect on the processing of potatoes. (author)

  18. Accumulation of solvent-soluble and solvent-insoluble antioxidant phenolics in edible bean sprouts: implication of germination

    Directory of Open Access Journals (Sweden)

    Ren-You Gan

    2016-08-01

    Full Text Available Background: Edible bean sprouts are popular fresh vegetables widely recognized for their nutritional quality. However, while their antioxidant capacity and phenolic composition in both solvent-soluble and solvent-insoluble extracts has not been systematically evaluated. Methods: The antioxidant capacity and phenolic composition in both solvent-soluble and solvent-insoluble fractions of 12 cultivars of edible bean sprouts were evaluated, and relationships of antioxidant capacity and total phenolic content were also analyzed. Results: Sprouts demonstrated a wide range of antioxidant capacity and total phenolic content, with lower but substantial antioxidant capacity and total phenolic content in the solvent-insoluble fractions. Highest levels were found in the green mung bean sprout. Phenolic compounds, such as catechin, ellagic acid, ferulic acid, gallic acid and p-coumaric acid were widely detected in these sprouts. Additionally, a positive correlation was discovered between antioxidant capacity and total phenolic content in these edible bean sprouts. Conclusions: Germination generally resulted in the accumulation of antioxidant phenolics in the most edible bean sprouts. Edible bean sprouts with high antioxidant phenolics can be valuable natural sources of dietary antioxidants for the prevention of oxidative stress-related chronic diseases.

  19. Optimisation of wheat-sprouted soybean flour bread using response ...

    African Journals Online (AJOL)

    The effect of sprouted soybean flour on wheat bread was studied. Sprouting significantly increased the vitamin C content of soybean flour from 2.0 mg kg-1 to 3.25 mg kg-1. The sprouted soybean flour resulted in increased loaf volume, a firmer, spongy and more elastic loaf. However, increasing the sprouted soybean flour ...

  20. Studies on the storage of irradiated potatoes

    International Nuclear Information System (INIS)

    Sharabash, M.; Orabi, I.O.; Eloksh, I.I.; Abd-Alia, M.A.

    1995-01-01

    Tubers of alpha and king edward potato cultivars were exposed to O, 10 or 50 krad and stored under room temperature and good ventilation in perforated standard carton boxes. 10 Krad was the promising dosage for sprouting inhibition for both the two cultivars. Total losses in weight were partially dependent on the volume of the dosage, the time elapsed after irradiation and cultivar used. Ascorbic acid slightly decreased, whilst sugar fractions were increased during storage in irradiated tubers. Sugar fractions were sharply decreased in boiled or fried potatoes. Also, chlorogenic acid was increased by prolonging the storage time and/or increasing the exposure dose. After-cooking discoloration (darkening) was reduced by gamma irradiation and/or extending the storage period. Using 0.5% citric acid or 0.5% sodium citrate solution inhibited the after-cooking discoloration. 8 tabs

  1. Sprout inhibition in roots, tubers and bulbs; Inhibicion de brotes en raices, tuberculos y bulbos

    Energy Technology Data Exchange (ETDEWEB)

    Luna C, P.C

    1992-05-15

    The treatment with ionizing radiations to low dose impedes that appear sprouts in the tubers (potatoes); bulbs (onion and garlic) and in roots like the ginger and the yucca. The purpose is to inhibit the germination during the process of manipulation and storage, and this way to avoid the lost ones post crop of these products. The radiation dose required to inhibit the germination goes to depend of: the development conditions, the differences of variety, of the storage state of the bulbs and the conditions of cured and storage. (Author)

  2. Potato life-cycle : from genes to promoters to cis-acting elements

    NARCIS (Netherlands)

    Trindade, L.M.

    2003-01-01

    Potato plants undergo several developmental stages during their life-cycle, including stolon formation, tuber initiation, tuber growth, dormancy and sprouting. Most of these stages are continuous processes, meaning that different developmental stages can occur simultaneously in one

  3. Effect of gamma radiation and formulated essential oils on stored potato tubers

    International Nuclear Information System (INIS)

    Abo-El Seoud, M.A.; Helal, I.M.M.; Sarhan, M.M.; Galal, A. M.

    2010-01-01

    Potato tubers were irradiated with gamma radiation at doses of 0, 0.5, 0.15, 0.3 and 0.6 K-Gy. Irradiated and non-irradiated tubers were soaked in biocides formulated from essential oils of fennel, peppermint and caraway oil. All treated and non-treated tubers were stored at room temperature (20 ±1 degree C) for period of 150 days. Samples were taken every 30 days for different quality determinations. Results showed that non-treated tubers were decayed and appear to be sprouted continuously alongside the different storage periods. However, exposing the studied tubers to gamma radiation induced a promising effect for retarding this decay and sprouting. Moreover, soaking potato tubers in the formulated biocides induced further interruption for tubers decay and sprouting. In addition, the applied treatment exhibited other positive effects for keeping the good quality of the studied tubers during storage.

  4. Effect of the gamma radiation on the preservation pf potato tubers (Solanum tuberosum L.) during the storing period

    International Nuclear Information System (INIS)

    Mazon matanzo, M.P.; Fernandez Gonzalez, J.

    1976-01-01

    The effect of the gamma radiation on the preservation of potato tubers stored during a period of six months is described by evaluating the loss by sprouting, transpiration and infections. The dose of 9 Krad inhibits sprouting with independence of the epoch in wich the treatment is made, on the other hand a dose of 6 Krad inhibits sprouting only if the tubers are irradiated during the resting period. The treatment with IPC gave similar results to those obtained with a dose of 9 Krad. (author) [es

  5. Composition and physiological profiling of sprout-associated microbial communities

    Science.gov (United States)

    Matos, Anabelle; Garland, Jay L.; Fett, William F.

    2002-01-01

    The native microfloras of various types of sprouts (alfalfa, clover, sunflower, mung bean, and broccoli sprouts) were examined to assess the relative effects of sprout type and inoculum factors (i.e., sprout-growing facility, seed lot, and inoculation with sprout-derived inocula) on the microbial community structure of sprouts. Sprouts were sonicated for 7 min or hand shaken with glass beads for 2 min to recover native microfloras from the surface, and the resulting suspensions were diluted and plated. The culturable fraction was characterized by the density (log CFU/g), richness (e.g., number of types of bacteria), and diversity (e.g., microbial richness and evenness) of colonies on tryptic soy agar plates incubated for 48 h at 30 degrees C. The relative similarity between sprout-associated microbial communities was assessed with the use of community-level physiological profiles (CLPPs) based on patterns of utilization of 95 separate carbon sources. Aerobic plate counts of 7.96 +/- 0.91 log CFU/g of sprout tissue (fresh weight) were observed, with no statistically significant differences in microbial cell density, richness, or diversity due to sprout type, sprout-growing facility, or seed lot. CLPP analyses revealed that the microbial communities associated with alfalfa and clover sprouts are more similar than those associated with the other sprout types tested. Variability among sprout types was more extensive than any differences between microbial communities associated with alfalfa and clover sprouts from different sprout-growing facilities and seed lots. These results indicate that the subsequent testing of biocontrol agents should focus on similar organisms for alfalfa and clover, but alternative types may be most suitable for the other sprout types tested. The inoculation of alfalfa sprouts with communities derived from various sprout types had a significant, source-independent effect on microbial community structure, indicating that the process of

  6. Modelling Tumor-induced Angiogenesis: Combination of Stochastic Sprout Spacing and Sprout Progression

    Directory of Open Access Journals (Sweden)

    Hosseini F.

    2017-09-01

    Full Text Available Background: Angiogenesis initiated by cancerous cells is the process by which new blood vessels are formed to enhance oxygenation and growth of tumor. Objective: In this paper, we present a new multiscale mathematical model for the formation of a vascular network in tumor angiogenesis process. Methods: Our model couples an improved sprout spacing model as a stochastic mathematical model of sprouting along an existing parent blood vessel, with a mathematical model of sprout progression in the extracellular matrix (ECM in response to some tumor angiogenic factors (TAFs. We perform simulations of the siting of capillary sprouts on an existing blood vessel using finite difference approximation of the dynamic equations of some angiogenesis activators and inhibitors. Angiogenesis activators are chemicals secreted by hypoxic tumor cells for initiating angiogenesis, and inhibitors of the angiogenesis are chemicals that are produced around every new sprout during tumor angiogenesis to inhibit the formation of further sprouts as a feedback of sprouting in angiogenesis. Moreover, for modelling sprout progression in ECM, we use three equations for the motility of endothelial cells at the tip of the activated sprouts, the consumption of TAF and the production and uptake of Fibronectin by endothelial cells. Results: Coupling these two basic models not only does provide a better time estimation of angiogenesis process, but also it is more compatible with reality. Conclusion: This model can be used to provide basic information for angiogenesis in the related studies. Related simulations can estimate the position and number of sprouts along parent blood vessel during the initial steps of angiogenesis and models the process of sprout progression in ECM until they vascularize a tumor.

  7. Effects of gamma radiation and storage time on the physical, chemical and sensory qualities of ''Georgia Jet'' sweet potatoes

    International Nuclear Information System (INIS)

    Yakubu, P.I.

    1986-12-01

    Effect of gamma radiation and storage time on sprouting, rotting, changes in nutritional composition and sensory quality of Georgia Jet Sweet Potatoes were studied. All radiation doses used in the study inhibited sprouting. Sweet potatoes were severely injured at doses of 1.5 and 2.0 kGy and these doses affected their storage life and sensory quality. There was no significant difference in nutrient composition of sweet potatoes irradiated at doses of 0.8, 0.5, 0.1 kGy and the control. A dose of gamma rays of 0.5 kGy and above may not be desirable for sweet potatoes. The optimum dose may lie between 0.5 and 0.1 kGy

  8. Long-term storage of irradiated potatoes for processing use, 4

    International Nuclear Information System (INIS)

    Aoki, Shohei; Umeda, Keiji; Kameyama, Kenji.

    1984-01-01

    In order to develop suitable storage conditions in potatoes for processing use, non-irradiated and irradiated potatoes var. Norin No. 1 were stored in containers and in bulk. The contents of reducing sugars were determined and the properties of potato chips were analysed. Reconditioning at 10 deg C and RH 90 % for 4 weeks during an early period of dormancy lowered the content of reducing sugars remarkably in both the non-irradiated and irradiated potatoes which were either stored in containers or in bulk. The optimum conditions for the storage of potatoes were 8 deg C and RH 90 %, which enabled the potatoes to retain their hardness while the content reducing sugars decreased. The potatoes stored under the above conditions gave chips with a fairly good color. In contrast, the non-irradiated potatoes stored for a period of 6 months or longer sprouted and aged, resulting in chips with unsatisfactory color. It was found necessary to use potatoes with low contents of both amino acids and reducing sugars to avoid the browning of chips. Bulk storage which was found to be effective for air circulation through potatoes caused creeping phenomenon. (author)

  9. Batch scale storage of sprouting foods by irradiation combined with natural low temperature; pt. 1

    International Nuclear Information System (INIS)

    Cho, H.O.; Kwon, J.H.; Yang, H.S.; Byun, M.W.; Lee, C.H.

    1982-01-01

    In order to develop the commercial storage method of potatoes by irradiation combined with natural low temperature, two varieties of potatoes, Irish cobbler and Shimabara were stored at natural low temperature storage room (450x650x250cm; year round temperature change, 2-17 degC; 70-85% R.H.) on a batch scale followed by irradiation with optimum dose level. Irish cobbler and Shimabara were 100% sprouted after 3 months storage in control, whereas in 15Krad irradiated group, sprouting was completely inhibited at Irish cobbler for 9 months storage, and at Shimabara for 12 months. The extent of loss due to rot attack after 9 months storage was 6% in control, 6-8% in 10-15Krad irradiated group at Irish cobbler and weight loss was 16.5% in control, 5.1-5.6% in irradiated group, whereas rotting rate of Shimabara after 12 months storage was 100% in control, 15% in irradiated group and the weight loss of its was 12.6% in control, 7.3-7.4% in irradiated group. The moisture content in whole storage period of two varieties were 72-82% without remarkable changes. The total sugar and ascorbic acid contents were slightly decreased according to the dose increase and elapse of storage period, whereas reducing sugar content was increased. Irish cobbler was 90% markrtable after 9 months storage and 85% in Shimabara after 12 months storage. (Author)

  10. Screening Potato Varieties for Cultivation in Arid Regions: Effect of Planting Date on Emergence of Imported and Locally-Produced Seeds

    Directory of Open Access Journals (Sweden)

    I. Khan

    2001-01-01

    Full Text Available Potato is new as a major crop in arid regions. Due to its food value, expanding production and increasing consumer demand, it is being considered as a strategic crop. Production is based on yearly import of seed potatoes from Europe. Productivity of potato could be increased by targeted selection of varieties for local adaptation, by choosing the adequate conditions for planting and through proper seed management. The seed and its imposed planting time were found to be incompatible with optimum stand development for the crop, resulting in a loss of potential yield. The young physiological age and persisting dormancy of seed prevented early emergence of sprouts from a mid-November planting time. When planted on 11 consecutive dates, varieties showed a significant improvement of emergence rates with delayed planting time. Emergence rate doubled as planting was delayed from mid November to mid December. Emergence rate results for imported tubers were confirmed over three consecutive growing seasons and contrast with emergence data for locally produced seed planted at the same or earlier dates.

  11. SproutCore web application development

    CERN Document Server

    Keating, Tyler

    2013-01-01

    Written as a practical, step-by-step tutorial, Creating HTML5 Apps with SproutCore is full of engaging examples to help you learn in a practical context.This book is for any person looking to write software for the Web or already writing software for the Web. Whether your background is in web development or in software development, Creating HTML5 Apps with SproutCore will help you expand your skills so that you will be ready to apply the software development principles in the web development space.

  12. Effects of magnetic fields pretreatment of mungbean seeds on sprout ...

    African Journals Online (AJOL)

    Effects of magnetic fields pretreatment of mungbean seeds on sprout yield and quality. Yi-Ping Chen, Jun-Min He, Ran Li. Abstract. The aim of this investigation was to determine the effect of magnetic field pretreatment of mungbean seeds on the yield and quality of sprout. The sprout elongation, biomass and nutrition ...

  13. Effects of concentration and propagule age on the efficacy of gibberellic acid for breaking dormancy in potato minitubers

    NARCIS (Netherlands)

    Salimi, Kh.; Hosseini, M.B.; Tavakkol-Afshari, R.; Struik, P.C.

    2010-01-01

    The effects of a post-harvest application of gibberellic acid (GA3) at various concentrations (0, 10, 25, 50, or 100 mg l-1) and for different periods of incubation (1, 2, or 3 h) on the breaking of dormancy and on sprouting in potato (Solanum tuberosum L., cv. Marfona) minitubers of two ages

  14. Formation of dry gram-negative bacteria biocontrol products and small pilot tests against potato dry rot

    Science.gov (United States)

    Pseudomonas fluorescens strains S11:P:12, P22:Y:05, and S22:T:04 reduce important potato maladies in storage including dry rot, late blight, pink rot, and sprouting. Experiments were conducted to identify methods for producing a dried, efficacious biological control product from one or more of these...

  15. Sprouting of dormant buds on border trees

    Science.gov (United States)

    G.R., Jr. Trimble; H. Clay Smith; H. Clay Smith

    1970-01-01

    As part of an evaluation of silvicultura1 systems used in managing Appalachian hardwoods, we are studying degrade of border trees surrounding harvest-cut openings made in the patch cutting and group selection systems. One facet of this research dealt with determining what portion of visually evident dormant buds on border tree boles sprouted when the openings were cut...

  16. Profitability of precommercially thinning oak stump sprouts

    Science.gov (United States)

    John P. Dwyer; Daniel C. Dey; William B. Kurtz

    1993-01-01

    Thinning oak stump sprouts to a single stem at an early age will increase diameter growth of the released stem. However, precommercial thinning represents a substantial investment which must be carried for many years before any returns are realized. We estimated the incremental gains in yield and the present net worth for five crop-tree release treatments of 5-year-old...

  17. Origin of buds, branches, and sprouts

    Science.gov (United States)

    Kevin T. Smith

    2014-01-01

    Recent research shows that survivor trees in rural, managed forests rebuild broken crowns with new branches and foliage after ice storm injury (Shortle et al. 2014). Veteran trees in historic parks and landscapes show repeated cycles of crown loss and recovery (Fay 2002). Crown rebuilding or reiteration from sprouts is a physiological response with architectural...

  18. Fumigant toxicities of essential oils and two monoterpenes against potato tuber moth (Phthorimaea operculella Zeller

    Directory of Open Access Journals (Sweden)

    Tayoub Ghaleb

    2016-12-01

    Full Text Available Introduction: The potato tuber moth (PTM is the major economic pest of potato. Different approaches were tried to prevent and control this pest including natural pesticides and synthetic fumigants.

  19. Light influence in the nutritional composition of Brassica oleracea sprouts.

    Science.gov (United States)

    Vale, A P; Santos, J; Brito, N V; Peixoto, V; Carvalho, Rosa; Rosa, E; Oliveira, M Beatriz P P

    2015-07-01

    Brassica sprouts are considered a healthy food product, whose nutritional quality can be influenced by several factors. The aim of this work was to monitor the nutritional composition changes promoted by different sprouting conditions of four varieties of Brassica oleracea (red cabbage, broccoli, Galega kale and Penca cabbage). Sprouts were grown under light/darkness cycles and complete darkness. Standard AOAC methods were applied for nutritional value evaluation, while chromatographic methods with UV-VIS and FID detection were used to determine the free amino acids and fatty acids, respectively. Mineral content was analyzed by atomic absorption spectrometry. Sprouts composition revealed them as an excellent source of protein and dietary fiber. Selenium content was one of the most distinctive feature of sprouts, being the sprouting conditions determinant for the free amino acid and fatty acids profile. The use of complete darkness was beneficial to the overall nutritional quality of the brassica sprouts studied. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Changes of endogenous hormones in irradiated potato tubers

    International Nuclear Information System (INIS)

    Farag, S.E.A.; El-Saeid, H.M.; Abou-Hadid, A.F.

    1992-01-01

    Potato tubers Solatum tuberosum L. cv. Alpha were irradiated with 0.12 kgy and stored at room temperature. The endogenous hormones were extracted and determined using bioassay at dormant, budding and sprouting stages. The studied promoters were IAA, IAN, GA3 and Gibberellin like substances besides the inhibitors which included ABA and B-inhibitors. The results indicated that IAA was more sensitive to irradiation than IAN, GA3 but ABA was more stable than B-inhibitors during the dormancy. Irradiation decreased IAA and Gibberellin like substances and B-inhibitors but no change was observed in ABA content at dormant period. Irradiation caused a balance between promoters inhibitors at the end of tuber storage and after tubers sprouting

  1. Potato production in Thailand

    Science.gov (United States)

    Potato production has increased dramatically in recent years in Thailand. Consumer demand for fresh and processed potatoes has driven this trend. Most potatoes are produced in northern Thailand in either double cropping highland zones or as a single winter crop following rice in lowland regions. Maj...

  2. Exogenous ethylene inhibits sprout growth in onion bulbs

    Science.gov (United States)

    Bufler, Gebhard

    2009-01-01

    Background and Aims Exogenous ethylene has recently gained commercial interest as a sprouting inhibitor of onion bulbs. The role of ethylene in dormancy and sprouting of onions, however, is not known. Methods A cultivar (Allium cepa ‘Copra’) with a true period of dormancy was used. Dormant and sprouting states of onion bulbs were treated with supposedly saturating doses of ethylene or with the ethylene-action inhibitor 1-methylcyclopropene (1-MCP). Initial sprouting was determined during storage at 18 °C by monitoring leaf blade elongation in a specific size class of leaf sheaths. Changes in ATP content and sucrose synthase activity in the sprout leaves, indicators of the sprouting state, were determined. CO2 and ethylene production of onion bulbs during storage were recorded. Key results Exogenous ethylene suppressed sprout growth of both dormant and already sprouting onion bulbs by inhibiting leaf blade elongation. In contrast to this growth-inhibiting effect, ethylene stimulated CO2 production by the bulbs about 2-fold. The duration of dormancy was not significantly affected by exogenous ethylene. However, treatment of dormant bulbs with 1-MCP caused premature sprouting. Conclusions Exogenous ethylene proved to be a powerful inhibitor of sprout growth in onion bulbs. The dormancy breaking effect of 1-MCP indicates a regulatory role of endogenous ethylene in onion bulb dormancy. PMID:18940850

  3. Economic feasibility studies on radiation preservation of dried and cured fishery products, onions and potatoes

    International Nuclear Information System (INIS)

    Ahmed, M.; Karim, A.; Quaiyum, M.A.; Bhuiya, A.D.; Matin, M.A.; Siddiqui, A.K.; Hossain, M.M.

    1989-01-01

    Dried and cured fishery products, onions and potatoes face enormous storage losses in Bangladesh due to insect infestation and sprouting. Research and development work was carried out to assess the suitability of introducing irradiation processing of these products in the country. Experiments showed that a dose of 0.04-0.68 kGy could inhibit sprouting in onions. Sprouting in potatoes could be inhibited at 0.10 kGy. Dried and cured fishery products could be disinfested of insects at a dose of 0.30 kGy. Infrastructure such as transportation, storage, marketing and existing systems of post-harvest handling were analysed. Post-harvest storage losses of onion and dried fish were more than 50% after 6 months of storage. Potatoes could not be kept at ambient conditions for over 3 months after the harvesting season. Irradiation of onions and dried fish, if they were stored in suitable conditions after proper packaging, could save significant storage losses. Irradiated potatoes could be stored at 14 deg. C instead of 2-4 deg. C as practised normally. On the basis of the data collected on dried and cured fishery products, onions and potatoes, economic feasibility studies were conducted. Assumptions for calculation of cost of the irradiation facility were: (i) strength of the irradiator source: 7.40 PBq of 60 Co; (ii) construction period: 2 years; (iii) operating time: 7200 hours per year; (iv) economic life: 20 years; (v) capacity utilization: 80-90%. In addition to dried and cured fish, potatoes and onions, this facility would also treat fresh fish and medical products in order to maximize its use. It would have an investment cost of US $1.9 million. The payback period was found to be less than 4 years. If additional warehouses could be built along with the facility, such a venture would be more profitable. 23 refs, 8 figs, 28 tabs

  4. Sprouting inhibition of rhizomes by gamma irradiation

    International Nuclear Information System (INIS)

    Hilmy, Nazly; Chosdu, Rahayu

    1985-01-01

    Sprouting inhibition by gamma irradiation to prolong the storage life of 4 species of rhizomes, namely curcuma domestica, kaemferia galanga, curcuma xanthoriza and curcuma aeruginosa, has been carried out. Two groups of samples were used, freshly harvested rhizomes and fresh rhizomes which have been stored for about two weeks. The samples were packed in a plastic net bag, each contained about 100 grams of rhizomes. Irradiation was carried out at room temperature at the doses of 0, 0.02, 0.04, 0.06, 0.08, 0.10, 0.15, 0.20 and 0.25 kGy. Post irradiation storage was done at room temperature with relative humidity ranging between 85 and 95%. The results showed that irradiation doses of 0.06 to 0.08 kGy was sufficient to inhibit sprouting of freshly harvested rhizomes and prolonged its storage life for 6 weeks, while in the other group sprouting still occured at the dose of 0.25 kGy. Irradiation dose up to 0.25 kGy did not cause significant effect on moisture and volatile oil contents, as well as volatile oil characteristics of the samples. About 50% of weight losses were found either in irradiated or unirradiated samples after being stored for 8 weeks. Odour and texture were evaluated organoleptically while mould growth and insect damage were observed visually. (author)

  5. Pathogen detection, testing, and control in fresh broccoli sprouts

    Directory of Open Access Journals (Sweden)

    Fahey Jed W

    2006-04-01

    Full Text Available Abstract Background The recent increased interest in consuming green vegetable sprouts has been tempered by the fact that fresh sprouts can in some cases be vehicles for food-borne illnesses. They must be grown according to proper conditions of sanitation and handled as a food product rather than as an agricultural commodity. When sprouts are grown in accordance with the criteria proposed from within the sprout industry, developed by regulatory agencies, and adhered to by many sprouters, green sprouts can be produced with very low risk. Contamination may occur when these guidelines are not followed. Methods A one year program of microbial hold-and-release testing, conducted in concert with strict seed and facility cleaning procedures by 13 U.S. broccoli sprout growers was evaluated. Microbial contamination tests were performed on 6839 drums of sprouts, equivalent to about 5 million consumer packages of fresh green sprouts. Results Only 24 (0.75% of the 3191 sprout samples gave an initial positive test for Escherichia coli O157:H7 or Salmonella spp., and when re-tested, 3 drums again tested positive. Composite testing (e.g., pooling up to 7 drums for pathogen testing was equally sensitive to single drum testing. Conclusion By using a "test-and-re-test" protocol, growers were able to minimize crop destruction. By pooling drums for testing, they were also able to reduce testing costs which now represent a substantial portion of the costs associated with sprout growing. The test-and-hold scheme described herein allowed those few batches of contaminated sprouts to be found prior to packaging and shipping. These events were isolated, and only safe sprouts entered the food supply.

  6. A review of therapeutic potentials of sweet potato: Pharmacological ...

    African Journals Online (AJOL)

    can generally be beneficial in the prevention or treatment of chronic diseases through its antioxidant, anti-inflammatory ... and antiulcer activities. Studies on the haematinic effect of potato leaves and their ability to enhance some haemotological ..... skinned sweet potato in type 2 diabetic patients has been linked to an ...

  7. A review of therapeutic potentials of sweet potato: Pharmacological ...

    African Journals Online (AJOL)

    Sweet potato (Ipomoea batatas) is a global food crop, now being recognized as a functional food due to several of its nutraceutical components. Several experimental studies have reported that sweet potato can generally be beneficial in the prevention or treatment of chronic diseases through its antioxidant, ...

  8. Detection of irradiated potato and garlic by thermoluminescence measurement

    International Nuclear Information System (INIS)

    Chung, H.W.; Kwon, J.H.

    1998-01-01

    Potato and garlic irradiated with gamma ray and electron beam at sprout-inhibition doses, 0.15 and 0.30 kGy were subjected to the detection whether they are irradiated or not by measuring thermoluminescence(TL) for the minerals adhering to the samples. Minerals extracted from the samples showed a high correlation coefficients between absorbed doses and corresponding TL responses. Nonirradiated samples, however, did not exhibit characteristic TL glowcurves. Major glowcurve peaks were observed at 200 to in all irradiated samples. TL intensity was proportional to irradiated doses, but it varied with the samples tested even at the same dose. It can be concluded from the results that detection of irradiated potato and garlic is possible by measuring TL for extracted minerals from the unknown samples

  9. Increasing Antioxidant Content of Broccoli Sprouts Using Essential Oils During Cold Storage

    Directory of Open Access Journals (Sweden)

    El-Awady Aml A.

    2016-10-01

    Full Text Available Broccoli sprouts are natural functional foods for cancer prevention because of their high content of glucosinolate and antioxidant. Sprouts and mature broccoli are of potential importance in devising chemoprotective strategies in humans. The aim of the investigation was to study the effect of essential oils on broccoli seed germination, increase their antioxidant content and determine the glucosinolate concentration and other phytochemical parameters in 3-day-old sprouts during cold storage at 4°C and 95% RH for 15 days. The results showed that all treatments of essential oils increased germination index, seed germination percentage, seedling length, seedling vigour index, yield and the antioxidant content of broccoli sprout and reduced the microbial load compared to the control. Fortunately, the coliform bacteria was not detected in all treatments. Different essential oils of fennel, caraway, basil, thyme and sage were tested. The thyme oil was the best treatment, which increased the accumulation of the phenolic compounds and glucosinolate compared to the control at different storage periods. In the sprouts treated with thyme oil treatment and the control, at the end of cold storage, 1.98% and 28.06% of total phenolic content, 1.90% and 20.28% of 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging capacity, 1.39% and 58.33% of flavonoids, 1.93% and 36.25% of vitamin C, 2.95% and 22.02% of anthocyanin and 2.18% and 49.12% of glucosinolate were lost, respectively. A slight reduction differences in all detected compound concentrations occurred between the initial content and the end of storage period because of the application of thyme oil compared to the control. Therefore, the total glucosinolate level in the sprout (27.02 μg/g F.W. was higher than that in the florets (7.37 μg/g F.W.. Glucoraphanin was the most abundant aliphatic glucosinolate present in the sprout and reached the highest value (16.24 μg/g F.W. followed by glucoerucin (5

  10. Potato market in Ukraine

    Directory of Open Access Journals (Sweden)

    С. І. Мельник

    2017-06-01

    Full Text Available Purpose. To study Ukrainian potato market at the current stage of the development and determine its future prospects. Results. The features of Ukrainian potato market were determined. Production is almost fully provided by private households, meeting the needs of the domestic market. Main regions with the highest gross output and production of potatoes were defined. Ukraine is one of the major potato producing countries in the world. Today our country is not a key supplier or importer of this product because of the low export orientation of the industry, its technological backwardness, limited product range and the large number of small producers. Ukraine exports potato mainly to CIS countries, the highest share of potato import comes from the European Union. Now there are only a few large manufacturing companies in the market, which can be classified as industrial. Most potato varieties, officially permitted for dissemination in Ukraine, are classified as table ones and recommended for cultivation in the Forest-Steppe and Polissia zones. Achievements of the industry include the development of such very popular and promising trend as organic potato growing, which area in our country is one of the largest in the world. Conclusions. Potato produced in Ukraine is used for human consumption, animal feeding, planting and processing, its volumes are relatively stable. Large-scale industrial production of potato is not widely practiced because of low wholesale prices and high labor intensity of the cultivation process. During next few years, in view of current trends, production of potato and severe limitations of the domestic market for foreign operations will remain unchanged. A shift in emphasis in the product range – from fresh potato to processed food products should be a prospect for domestic industrial producers to improve their position in Ukraine and abroad.

  11. Early Sprouts: Cultivating Healthy Food Choices in Young Children

    Science.gov (United States)

    Kalich, Karrie; Bauer, Dottie; McPartlin, Deirdre

    2009-01-01

    Plant lifelong healthy eating concepts in young children and counteract the prevalence of childhood obesity with "Early Sprouts." A research-based early childhood curriculum, this "seed-to-table" approach gets children interested in and enjoying nutritious fruits and vegetables. The "Early Sprouts" model engages…

  12. Impact of diets containing graded level of malted sorghum sprout ...

    African Journals Online (AJOL)

    An experiment was conducted to determine the impact of diets containing graded levels of malted sorghum sprout mixed with pineapple waste (MSPW) on rumen fermentation profile of West African dwarf goats using in vitro gas production techniques. The malted sorghum sprout and pineapple waste mixture were obtained ...

  13. Effect Of Sprouting On Available Lysine Content Of Cowpea ( Vigna ...

    African Journals Online (AJOL)

    This study was conducted to determine the effect of sprouting on available Lysine content of cowpea (Vigna unguiculata) flour and the performance of the flour used for producing “moi – moi” (steamed bean cake). Cowpea seed was subjected to sprouting for different periods of 1 day, 2 days and 3 days for samples B, C and ...

  14. A Local Inflammatory Renin-Angiotensin System Drives Sensory Axon Sprouting in Provoked Vestibulodynia.

    Science.gov (United States)

    Liao, Zhaohui; Chakrabarty, Anuradha; Mu, Ying; Bhattacherjee, Aritra; Goestch, Martha; Leclair, Catherine M; Smith, Peter G

    2017-05-01

    Vestibulodynia is a form of provoked vulvodynia characterized by profound tenderness, hyperinnervation, and frequently inflammation within well-defined areas of the human vestibule. Previous experiments in animal models show that inflammatory hypersensitivity and hyperinnervation occur in concert with establishment of a local renin-angiotensin system (RAS). Moreover, mechanical hypersensitivity and sensory axon sprouting are prevented by blocking effects of angiotensin II on angiotensin II receptor type 2 (AT2) receptors. This case-control study assessed whether a RAS contributes to hyperinnervation observed in human vestibulodynia. Vestibular biopsies from asymptomatic controls or patients' nontender areas showed moderate innervation and small numbers of inflammatory cells. In women with vestibulodynia, tender areas contained increased numbers of mechanoreceptive nociceptor axons, T-cells, macrophages, and B-cells, whereas mast cells were unchanged. RAS proteins were increased because of greater numbers of T cells and B cells expressing angiotensinogen, and increased renin-expressing T cells and macrophages. Chymase, which converts angiotensin I to angiotensin II, was present in constant numbers of mast cells. To determine if tender vestibular tissue generates angiotensin II that promotes axon sprouting, we conditioned culture medium with vestibular tissue. Rat sensory neurons cultured in control-conditioned medium showed normal axon outgrowth, whereas those in tender tissue-conditioned medium showed enhanced sprouting that was prevented by adding an AT2 antagonist or angiotensin II neutralizing antibody. Hypersensitivity in provoked vestibulodynia is therefore characterized by abnormal mechanonociceptor axon proliferation, which is attributable to inflammatory cell-derived angiotensin II (or a closely related peptide) acting on neuronal AT2 receptors. Accordingly, reducing inflammation or blocking AT2 represent rational strategies to mitigate this common pain

  15. Post-translational regulation of acid invertase activity by vacuolar invertase inhibitor affects resistance to cold-induced sweetening of potato tubers.

    Science.gov (United States)

    McKenzie, Marian J; Chen, Ronan K Y; Harris, John C; Ashworth, Matthew J; Brummell, David A

    2013-01-01

    Cold-induced sweetening (CIS) is a serious post-harvest problem for potato tubers, which need to be stored cold to prevent sprouting and pathogenesis in order to maintain supply throughout the year. During storage at cold temperatures (below 10 °C), many cultivars accumulate free reducing sugars derived from a breakdown of starch to sucrose that is ultimately cleaved by acid invertase to produce glucose and fructose. When affected tubers are processed by frying or roasting, these reducing sugars react with free asparagine by the Maillard reaction, resulting in unacceptably dark-coloured and bitter-tasting product and generating the probable carcinogen acrylamide as a by-product. We have previously identified a vacuolar invertase inhibitor (INH2) whose expression correlates both with low acid invertase activity and with resistance to CIS. Here we show that, during cold storage, overexpression of the INH2 vacuolar invertase inhibitor gene in CIS-susceptible potato tubers reduced acid invertase activity, the accumulation of reducing sugars and the generation of acrylamide in subsequent fry tests. Conversely, suppression of vacuolar invertase inhibitor expression in a CIS-resistant line increased susceptibility to CIS. The results show that post-translational regulation of acid invertase by the vacuolar invertase inhibitor is an important component of resistance to CIS. © 2012 Blackwell Publishing Ltd.

  16. Assuring Potato Tuber Quality during Storage: A Future Perspective

    Directory of Open Access Journals (Sweden)

    M. C. Alamar

    2017-11-01

    Full Text Available Potatoes represent an important staple food crop across the planet. Yet, to maintain tuber quality and extend availability, there is a necessity to store tubers for long periods often using industrial-scale facilities. In this context, preserving potato quality is pivotal for the seed, fresh and processing sectors. The industry has always innovated and invested in improved post-harvest storage. However, the pace of technological change has and will continue to increase. For instance, more stringent legislation and changing consumer attitudes have driven renewed interest in creating alternative or complementary post-harvest treatments to traditional chemically reliant sprout suppression and disease control. Herein, the current knowledge on biochemical factors governing dormancy, the use of chlorpropham (CIPC as well as existing and chemical alternatives, and the effects of pre- and post-harvest factors to assure potato tuber quality is reviewed. Additionally, the role of genomics as a future approach to potato quality improvement is discussed. Critically, and through a more industry targeted research, a better mechanistic understanding of how the pre-harvest environment influences tuber quality and the factors which govern dormancy transition should lead to a paradigm shift in how sustainable storage can be achieved.

  17. Assuring Potato Tuber Quality during Storage: A Future Perspective.

    Science.gov (United States)

    Alamar, M C; Tosetti, Roberta; Landahl, Sandra; Bermejo, Antonio; Terry, Leon A

    2017-01-01

    Potatoes represent an important staple food crop across the planet. Yet, to maintain tuber quality and extend availability, there is a necessity to store tubers for long periods often using industrial-scale facilities. In this context, preserving potato quality is pivotal for the seed, fresh and processing sectors. The industry has always innovated and invested in improved post-harvest storage. However, the pace of technological change has and will continue to increase. For instance, more stringent legislation and changing consumer attitudes have driven renewed interest in creating alternative or complementary post-harvest treatments to traditional chemically reliant sprout suppression and disease control. Herein, the current knowledge on biochemical factors governing dormancy, the use of chlorpropham (CIPC) as well as existing and chemical alternatives, and the effects of pre- and post-harvest factors to assure potato tuber quality is reviewed. Additionally, the role of genomics as a future approach to potato quality improvement is discussed. Critically, and through a more industry targeted research, a better mechanistic understanding of how the pre-harvest environment influences tuber quality and the factors which govern dormancy transition should lead to a paradigm shift in how sustainable storage can be achieved.

  18. Technology of irradiation processing for checking garlic sprouting

    International Nuclear Information System (INIS)

    Chen Yuntang

    1999-01-01

    Study on technology of irradiation processing for checking garlic sprouting was carried out. The results are as follows. 1. the suitable irradiation time of garlic could be prolonged when the garlic was preserved at low temperature (-2 +- 1 degree C). When the garlic was stored till the end of the year and then irradiated, the sprouting could be checked effectively. When the garlic was stored till the next March, the effect of sprouting inhibition decreased; 2. When garlic was irradiated at suitable time and then subsequently stored at low temperature, the effect of sprouting inhibition was the same as that with cold storage before irradiation. However, if the cold storage period was rather long, the effect was not as good as that with cold storage after irradiation; 3. Browning of irradiated garlic did not occur when the garlic was preserved at low temperature (-2 +- 1 degree C), indicating that the browning of sprout and bud was caused by higher storage temperature

  19. Antioxidant Activity of Phenolic Compounds from Fava Bean Sprouts.

    Science.gov (United States)

    Okumura, Koharu; Hosoya, Takahiro; Kawarazaki, Kai; Izawa, Norihiko; Kumazawa, Shigenori

    2016-06-01

    Fava beans are eaten all over the world and recently, marketing for their sprouts began in Japan. Fava bean sprouts contain more polyphenols and l-3,4-dihydroxyphenylalanine (l-DOPA) than the bean itself. Our antioxidant screening program has shown that fava bean sprouts also possess a higher antioxidant activity than other commercially available sprouts and mature beans. However, the individual constituents of fava bean sprouts are not entirely known. In the present study, we investigated the phenolic compounds of fava bean sprouts and their antioxidant activity. Air-dried fava bean sprouts were treated with 80% methanol and the extract was partitioned in water with chloroform and ethyl acetate. HPLC analysis had shown that the ethyl acetate-soluble parts contained phenolic compounds, separated by preparative HPLC to yield 5 compounds (1-5). Structural analysis using NMR and MS revealed that the compounds isolated were kaempferol glycosides. All isolated compounds had an α-rhamnose at the C-7 position with different sugars attached at the C-3 position. Compounds 1-5 had β-galactose, β-glucose, α-rhamnose, 6-acetyl-β-galactose and 6-acetyl-β-glucose, respectively, at the C-3 position. The amount of l-DOPA in fava bean sprouts was determined by the quantitative (1) H NMR technique. The l-DOPA content was 550.45 mg ± 11.34 /100 g of the raw sprouts. The antioxidant activities of compounds 2-5 and l-DOPA were evaluated using the 2,2-diphenyl-1-picrylhydrazyl scavenging assay. l-DOPA showed high antioxidant activity, but the isolated kaempferol glycosides showed weak activity. Therefore, it can be suggested that l-DOPA contributed to the antioxidant activity of fava bean sprouts. © 2016 Institute of Food Technologists®

  20. WEED MANAGEMENT AND CONTROL IN POTATOES

    Directory of Open Access Journals (Sweden)

    Marcelo Cleón de Castro Silva

    2011-07-01

    Full Text Available This review shows instructions to potatoes' farmer about behavior of the weeds and how to manage them so as to minimize loss of productivity through the use of control strategies for potato crop. The prevention consists in adoption of practices that prevents entry of unwanted species of weeds in the planting site. The control reduces the infestation of these species, but this practice does not eradicate them completely. However, it needs to control the weeds before the area preparation for planting the tubers until complete closure of the soil by shoots of potatoes during the critical period. After covering the soil, the potato crop does not suffer negative interference caused by weeds. The cultural practices include a good plane for harvest, plant crop rotation, the planting of appropriate plants for covering the soil, the ideal space to the planting and the correct time to potato planting. The control must be efficient to reduce the number of weeds in the area to avoid economic losses to farmers. It is necessary to establish weed management strategies in order to maintain sustainable farming systems, preserving the environment and quality of life of the farmer.

  1. Potatoes, pathogens and pests

    NARCIS (Netherlands)

    Lazebnik, Jenny

    2017-01-01

    Currently, fungicides are necessary to protect potato crops against late blight, Phytophthora infestans, one of the world’s most damaging crop pathogens. The introgression of plant resistance genes from wild potato species targeted specifically to the late blight pathogen into

  2. Preservation of potatoes and onions by irradiation and chemical treatments. A status report

    International Nuclear Information System (INIS)

    Sekhavat, A.; Zare, Z.; Kudva, M.G.; Chopra, K.L.; Shariat Panahi, J.M.

    1978-01-01

    Preservation of potatoes and onions is an urgent need in Iran, and an attempt has been made to gain experience in this field by applying ionizing radiation and chemicals. 60 Co gamma radiation doses of 5, 10, 12 and 15krad for three local varieties of potatoes and of 3, 6 and 9krad for one variety of onions, salicylic acid concentrations of 500, 1500 and 2500ppm, and combinations of different doses of radiation and salicylic acid were tried. Upon irradiation with doses of 12-15krad a significant reduction in the losses due to sprouting, rotting and dehydration was observed for all three varieties of potatoes after a storage period of nine months. Chemical treatment alone proved ineffective. Some doses of a combined treatment with salicylic acid plus irradiation yielded good results, but they need confirmation by further investigation. Organoleptic tests were carried out on two varieties of potatoes. Due to a delay in starting the treatment of onions after harvest, a high percentage had already begun to sprout and so the results obtained are only approximate values. (author)

  3. Plastid transformation in potato: Solanum tuberosum.

    Science.gov (United States)

    Valkov, Vladimir T; Gargano, Daniela; Scotti, Nunzia; Cardi, Teodoro

    2014-01-01

    Although plastid transformation has attractive advantages and potential applications in plant biotechnology, for long time it has been highly efficient only in tobacco. The lack of efficient selection and regeneration protocols and, for some species, the inefficient recombination using heterologous flanking regions in transformation vectors prevented the extension of the technology to major crops. However, the availability of this technology for species other than tobacco could offer new possibilities in plant breeding, such as resistance management or improvement of nutritional value, with no or limited environmental concerns. Herein we describe an efficient plastid transformation protocol for potato (Solanum tuberosum subsp. tuberosum). By optimizing the tissue culture system and using transformation vectors carrying homologous potato flanking sequences, we obtained up to one transplastomic shoot per bombardment. Such efficiency is comparable to that usually achieved in tobacco. The method described in this chapter can be used to regenerate potato transplastomic plants expressing recombinant proteins in chloroplasts as well as in amyloplasts.

  4. In vitro development of buds from tubers of potato (Solanum tuUerosum L.) subjected to gamma radiation

    International Nuclear Information System (INIS)

    Fernandez Gonzalez, J.; Garcia Collantes, M.A.

    1976-01-01

    The 'in vitro' development of buds from potato tubers subjected to gamma radiation at doses of 3, 6, 9 and 12 Krad is studied. The effect of radiation was dependent on the dormant stage of the buds. Intermediate doses (6-9 Krad) did inhibit mitotic division but not cellular elongation. When irradication is carried out at the end of the resting period, there is an apparent sprouting due to the elongation of previously formed cells. (author) [es

  5. INNOVATIVE SPECIFIC PRODUCTS: ORGANIC SPROUTS (MICROGREENS AND SEEDLINGS (BABY LEAVES

    Directory of Open Access Journals (Sweden)

    M. I. Ivanova

    2016-01-01

    Full Text Available The assortment of salad crops, process parameters for production of seedlings (Baby leaf and sprouts (Microgreens is presented. The principles of the selection and sale of salad crops in the local market are recommended.

  6. Effect of irradiation on the post-harvest life of potatoes

    International Nuclear Information System (INIS)

    Mahboob, F.; Badshah, N.; Jabeen, N.; Ayub, G.

    2004-01-01

    Research work was conducted to find out the effect of irradiation on the post-harvest life of potatoes. Cultivar Raja was obtained from Agricultural Research Institute, Tarnab, and irradiated by Cobalt-60 source at different doses 0, 5, 7.5, 10 and 15 Krad at the Nuclear Institute for Food and Agriculture (NIFA), Tarnab during the year 2002. The samples were then stored for three months at the Horticultural Research Farm, Malakandher, at a room temperature of 30-39 degree C and relative humidity of 29-63%. Various tests carried out at Food Science laboratory revealed that irradiation significantly affected the weight loss, sugars, starch, ascorbic acid, sprouting and specific gravity. It was observed that maximum sprouting has occurred in control (42.1%) followed by 5 Krad irradiated tubers (6.4%). While irradiation doses of 7.5, 10 and 15 Krad completely inhibited sprouting. Maximum percent decrease in weight (42.66%), reducing sugars (0.57%), non reducing sugars (0.87%), starch (12%), ascorbic acid (32%) and specific gravity (4%) were recorded for control while minimum percent decrease in weight (31.40%), reducing sugars (0.19%), non-reducing sugars (0.27%), starch (8.0%), ascorbic acid (12%) and specific gravity (1.7%) were noted for IS Krad irradiated tubers. Irradiation dose of 7.5 Krad seems to be better for the extension of shelf life of potatoes

  7. Effectiveness of a developed potato storage system in shelf life and nutritional quality compared to traditional practice in Bangladesh

    Directory of Open Access Journals (Sweden)

    Fahad Jubayer

    2017-01-01

    Full Text Available This research was carried out to achieve the objective of finding out the performance of a developed potato storage system in storage life and nutritional quality and comparing the system to the traditional practice in Bangladesh. A laboratory version of improved design of potato store and its construction was made at Bangladesh Agricultural University which consisted an evaporative cooling chamber, made of reinforced concrete cement; partially filled-in with water. Two varieties of potato (Diamant and Lal Pakri were used in this research. Both large and small types for each kind of potato varieties were taken. Experimental design was set up for both improved storage bin and farmer's traditional practice. Spoilage, sprouting, shrinkage, moisture content, vitamin C, and total sugar content of potato were determined for both methods of storage at a regular interval and results were compared. Economic benefit of the developed storage system over traditional practice was also determined. From the result of the study, it appeared that the improved potato storage bin was better in shelf life and nutritional quality for storing potato than the farmer's traditional practice.

  8. Effect of Storage Methods and Period on the Physiological and Nutrient Components of Livingstone Potato (Plectranthus esculentus in Abia State, Nigeria

    Directory of Open Access Journals (Sweden)

    Ezeocha Chinelo Vanessa

    2017-02-01

    Full Text Available Livingstone potato production in Nigeria is faced with the challenge of high postharvest losses. The aim of this study was to assess the effect of some storage methods on the physiological and nutritional quality of livingstone potato. Potatoes were separated and stored using eight different methods: potatoes stored in3x3x3 ft pits and covered with sand under the shade (T1, potatoes stored in3x3x3 ft pits and covered with ash under the shade (T2, potatoes stored in3x3x3 ft pits and covered with rice husk under the shade (T3, potatoes stored in3x3x3 ft pits and covered with a wood shavings under the shade (T4, potatoes spread on a bamboo platform under the shade(T5, potatoes spread on a raffia palm platform under the shade(T6, potatoes spread on concrete pavement inside the barn (T7, potatoes spread on the ground under the shade(T8, potatoes buried under the ground (T9. T8 was used as the control treatment. Temperature and relative humidity of the environment was monitored, the roots were weighed monthly to access the weight loss, sprouting and rot were observed visually and proximate composition of the stored roots were evaluated using standard methods. The lowest percentage weight loss was observed in samples stored in a pit covered with wood ash and those covered with river sand. Samples stored in pits and covered with wood shavings, wood ash and river sand had the lowest percentage of sprouting (1.63%, 3.45% and 6.06% respectively. The dry matter content increased with storage period in all the storage methods. The starch yield varied in the different storage methods with the samplescovered with river sand (13.45% and the samples buried underground in the field(10.53% giving the highest starch yield at the end of the storage period. Samples stored under rice husk had the highest ash content (4.77% while the crude fibre and crude protein contents were highest in the samples spread on raffia palm. The results showed that spreading on the floor

  9. Angiogenic sprouting is regulated by endothelial cell expression of Slug.

    Science.gov (United States)

    Welch-Reardon, Katrina M; Ehsan, Seema M; Wang, Kehui; Wu, Nan; Newman, Andrew C; Romero-Lopez, Monica; Fong, Ashley H; George, Steven C; Edwards, Robert A; Hughes, Christopher C W

    2014-05-01

    The Snail family of zinc-finger transcription factors are evolutionarily conserved proteins that control processes requiring cell movement. Specifically, they regulate epithelial-to-mesenchymal transitions (EMT) where an epithelial cell severs intercellular junctions, degrades basement membrane and becomes a migratory, mesenchymal-like cell. Interestingly, Slug expression has been observed in angiogenic endothelial cells (EC) in vivo, suggesting that angiogenic sprouting may share common attributes with EMT. Here, we demonstrate that sprouting EC in vitro express both Slug and Snail, and that siRNA-mediated knockdown of either inhibits sprouting and migration in multiple in vitro angiogenesis assays. We find that expression of MT1-MMP, but not of VE-Cadherin, is regulated by Slug and that loss of sprouting as a consequence of reduced Slug expression can be reversed by lentiviral-mediated re-expression of MT1-MMP. Activity of MMP2 and MMP9 are also affected by Slug expression, likely through MT1-MMP. Importantly, we find enhanced expression of Slug in EC in human colorectal cancer samples compared with normal colon tissue, suggesting a role for Slug in pathological angiogenesis. In summary, these data implicate Slug as an important regulator of sprouting angiogenesis, particularly in pathological settings.

  10. In situ conservation of wild potato germplasm in Argentina: Example and possibilities

    Directory of Open Access Journals (Sweden)

    Carlos F. Marfil

    2015-01-01

    Full Text Available The potato is the third most important food crop worldwide and in situ conservation of its wild relatives is considered an urgent priority. Although regions of the Americas with high wild potato species richness have been identified, the need to identify specific sites for establishing genetic reserves is still pending. Matching distribution data of Argentinean wild potato species to existing protected areas (PA, two priority sites were identified. The creation of genetic reserves in these two PA would make possible to preserve populations of species that have been successfully incorporated into the crop and are listed in the global priority Crop Wild Relative inventory. While the presence of target species in PA could ensure a passive conservation, in situ conservation programs require to actively intervene in selected areas. From a field study performed on populations of the wild potato Solanum kurtzianum naturally growing in a PA, the Villavicencio Natural Reserve (Mendoza province, a baseline with distribution, biotic interactions, sprouting behavior, population dynamics, AFLP and pollen viability data was established. Based on a systematic work in this Reserve we have generated a working protocol to be implemented at national and regional levels for the in situ conservation of potato wild relatives.

  11. Impedance measurement of irradiated potatoes: a method to identify radiation processing

    International Nuclear Information System (INIS)

    Mastro, N.L. del; Villavicencio, A.L.C.H.

    1992-01-01

    The potato is firmly established in many parts of the world as a major staple food. Then, radiation processing of potato is approved in many countries for sprouting inhibition and extension of shelf life in a dose range from about 0.01 to 0.15 kGy of 60 Co. The use of electrical conductance methods for the detection of Salmonella, some virus or the action of herbicides on plant has been reported and differences have been observed between instruments in respect of the magnitude of conductance change or rates of change in conductance response. A reliable technique to identify potatoes or other food products has not been established so far, though several methods have been reported. Electrical impedance might thus serve for characterization of unirradiated and irradiated tissues and cells. In this work, potato tubers from an European variety, named Bintje, grown in Sao Paulo State were employed. Potatoes were punctured with steel electrodes and impedance measured at different frequencies (1 k Hz-100 k Hz) by passing 3-5 m A alternating current through it. The impedance ratio of 50 k Hz/5 k Hz calculated from ten replicate samples decreases with the increment of the dose when doses of O 0.75 and 0.15 kGy from a Gamma Cell 220 were utilized. The impedance measurement were slightly affected by the place of puncture. (author)

  12. Characteristics of starch breads enriched with red potatoes

    Directory of Open Access Journals (Sweden)

    Dorota Gumul

    2017-01-01

    Full Text Available Starch breads may often be low in nutritional value, in comparison to traditional products, as they contain less dietary fibre, protein and micro and macro elements. As an effect a risk of mineral deficiencies and digestive problems caused by lack of dietary fibre could be expected in persons adhering to gluten free diet. To eliminate such problems, a continuous research on gluten-free bread nutritional enrichment, has been done in recent years. Raw material used to enrich gluten free products should include: inulin, lupine, radish, soy, lucerne sprouts, oilseeds, different type of dried fruits. Among the most commonly used raw materials, there are flours from gluten free cereals and pseudocereals such as buckwheat, amaranth and maize are very popular. It seem that valuable alternative could be considered a red, purple or pink potatoes as starch breads additives. The aim of this work was to investigate the effect addition of freeze-dried color potatoes on crude fiber, polyphenols, anthocyanins and flavonoids and nutritional value of gluten free breads. It could be concluded, that freeze-dried color (red, purple, pink potatoes enriched the gluten free breads (starch breads with health promoting bioactive components, like polyphenols, and highly valuable protein. The most promising additive was Magenta Love red potato variety lyophilisates, because gluten free breads enriched with this component were characterized by high protein content and the highest content of free and bound polyphenols, flavonoids, anthocyanins. The presence of all these components increased the nutritional and pro-health value of gluten free product as starch bread.

  13. Irradiation of onions with the commercial potato irradiator

    International Nuclear Information System (INIS)

    Aoki, Shohei; Kawashima, Koji; Hayasi, Toru

    1983-01-01

    Three varieties of onion harvested in Hokkaido were irradiated with the Shohoro Potato Irradiator on 29th September, 1981. One ton of each of the varieties, Kitamiki (KI), Ohohtsuku (OH) and Furanui (FU), was used in this investigation. Onions had longer dormancy period in the order of FU>OH>KI. Higher sprouting percentage was obtained in the unirradiated onions, while they were stored at a higher temperature or stored for a longer period. Generally, unirradiated onions sprouted before they were deteriorated. Thus the number of deteriorated bulbs in the unirradiated onions was superficially less than that in the irradiated ones. When the onions which were taken from warehouses on 26th March, 1982 were stored at room temperature, the percentage of wholesome bulbs was higher in KI and OH than FU. Small buds in some of the irradiated onions turned dark after a long storage time. Quantitative estimation of this phenomena is left to be resolved. There was little relationship between the weight loss and the number of wholesome onions. (author)

  14. Involvement of Bacterial Quorum-Sensing Signals in Spoilage of Bean Sprouts

    OpenAIRE

    Rasch, Maria; Andersen, Jens Bo; Nielsen, Kristian Fog; Flodgaard, Lars Ravn; Christensen, Henrik; Givskov, Michael; Gram, Lone

    2005-01-01

    Bacterial communication signals, acylated homoserine lactones (AHLs), were extracted from samples of commercial bean sprouts undergoing soft-rot spoilage. Bean sprouts produced in the laboratory did not undergo soft-rot spoilage and did not contain AHLs or AHL-producing bacteria, although the bacterial population reached levels similar to those in the commercial sprouts, 108 to 109 CFU/g. AHL-producing bacteria (Enterobacteriaceae and pseudomonads) were isolated from commercial sprouts, and s...

  15. Effects of Yeast Polysaccharide on Growth and Flavonoid Accumulation in Fagopyrum tataricum Sprout Cultures

    OpenAIRE

    Zhao, Gang; Zhao, Jianglin; Peng, Lianxin; Zou, Liang; Wang, Jingbo; Zhong, Lingyun; Xiang, Dabing

    2012-01-01

    The purpose of this study was to investigate the effects of yeast polysaccharide (YPS) on growth and flavonoid accumulation in sprout cultures of Fagopyrum tataricum (tartary buckwheat). Without obvious change in the appearance of the sprouts, the exogenous YPS notably stimulated the production of functional metabolites in F. tataricum sprouts, and the stimulation effect was concentration-dependent. With 400 mg/L of YPS applied to the sprout cultures ...

  16. Effect of irradiation on vitamin C content of strawberries and potatoes in combination with storage and with further cooking in potatoes

    International Nuclear Information System (INIS)

    Graham, W.D.; Stevenson, M.H.

    1997-01-01

    The vitamin C content of four varieties of strawberry was determined before and after treatment with ionising radiation at doses of 1, 2 or 3 kGy and after storage for 5 and 10 days at 6°C, and also in potatoes which, having been allowed a period of one month to recover from the effects of post-harvest stress, were irradiated at a sprout inhibition dose of 0.15 kGy, followed by storage and cooking. Total ascorbic acid (TAA), ascorbic acid (AA) and dehydroascorbic acid (DHAA) concentrations were measured using the technique of ion-exclusion high-performance liquid chromatography. Results from analysis of the strawberry samples showed the DHAA content increased immediately following irradiation and must, therefore, be taken into account when reporting vitamin C levels in irradiated produce. In addition it was observed that whilst irradiation did affect the vitamin C concentration in all varieties of strawberry, the change was small in comparison with the large variations observed between varieties. With regard to potatoes results showed that, whilst irradiation, storage and cooking all had the effect of reducing vitamin C concentration, irradiated samples stored for 5 months had similar or marginally higher levels than their non-irradiated counterparts. Cooking did not markedly reduce TAA content of irradiated potatoes compared to non-irradiated potatoes and it was also noted that microwave cooking was more destructive than boiling in lightly salted water

  17. Involvement of bacterial quorum-sensing signals in spoilage of bean sprouts

    DEFF Research Database (Denmark)

    Rasch, Maria; Andersen, Jens Bo; Nielsen, Kristian Fog

    2005-01-01

    sprouts. Thin-layer chromatography and liquid chromatography-high-resolution mass spectrometry revealed the presence of N-3-oxo-hexanoyl-l-homoserine lactone in spoiled bean sprouts and in extracts from pure cultures of bacteria. During normal spoilage, the pH of the sprouts increased due to proteolytic...

  18. Growth and quality of soybean sprouts (Glycine max L. Merrill) as affected by gamma irradiation

    Science.gov (United States)

    Soybean sprouts are considered as natural and healthy food by Asian consumers. However, sprouts are often associated with outbreaks of foodborne illnesses and recalls due to contamination of seeds with human pathogens. Irradiation may be used to inactivate pathogens on seeds and sprouts. In this stu...

  19. Oak sprouts grow better than seedlings under drought stress

    Czech Academy of Sciences Publication Activity Database

    Pietras, Justyna; Stojanović, Marko; Knott, R.; Pokorný, Radek

    2016-01-01

    Roč. 9, č. 4 (2016), s. 529-535 ISSN 1971-7458 R&D Projects: GA MŠk(CZ) EE2.3.20.0267 Institutional support: RVO:67179843 Keywords : drought stress * sap flow * transpiration * biomass Production * sessile Oak * sprout * seedling Subject RIV: EF - Botanics Impact factor: 1.623, year: 2016

  20. Effect of processing (sprouting and fermentation) of five local ...

    African Journals Online (AJOL)

    CLEMENT O BEWAJI

    ABSTRACT: The effect of processing (combined sprouting and fermentation) on chemical composition, tannin content, in vitro protein digestibility and mineral element composition of five local varieties were studied. The five varieties studied were: “chakalari white, chakalari red, yafimoro, tumbuna and mire”. The parameters ...

  1. Bud initiation and optimum harvest date in Brussels sprouts

    NARCIS (Netherlands)

    Everaarts, A.P.; Sukkel, W.

    1999-01-01

    For six cultivars of Brussels sprouts (Brassica oleracea var. gemmifera) with a decreasing degree of earliness, or optimum harvest date, the time of bud initiation was determined during two seasons. Fifty percent of the plants had initiated buds between 60 and 75 days after planting (DAP) in 1994

  2. Sprouted barley for dairy cows: Is it worth it

    Science.gov (United States)

    Sprouted grains have gained renewed interest among grazing dairy farmers in response to high grain prices, grain scarcity (in the organic dairy sector) and challenges in producing high-quality forages. This interest has been spurred by high-profile advertising by companies selling the systems, as we...

  3. Research on sprout inhibition effect of refrigerated garlic by irradiation

    International Nuclear Information System (INIS)

    Zhang Xuan; He Jianzhong; Li Ruijun

    2005-01-01

    This paper researches the sprout inhibition effect by irradiation on refrigerated garlic. The results shows that, the garlic is still in the period of dormancy within 7 days after taken out from the refrigerated warehouse, and irradiation have a good sprout inhibition effect on it. The irradiation dose is 40-90 Gy, the same as that of the post harvest irradiation treatment on garlic. Refrigerate the Zhongmu Garlic (at -2 degree C-0 degree C) until the middle ten days of February the next year, place it at the room temperature (10 degree C-15 degree C) for 1-7 days after taking it out of the warehouse, then use 60 Co γ-ray to irradiate it until the absorbed dose reaches 40-90 Gy, the sprout inhibition effect can be realized. The test also indicates that the deposited time after taking out of the refrigerated warehouse is crucial to the sprout inhibition effect of refrigerated garlic by irradiation. (authors)

  4. Factors affecting seed set in brussels sprouts, radish and cyclamen

    NARCIS (Netherlands)

    Murabaa, El A.I.M.

    1957-01-01

    If brussels sprouts were, self-fertilized, seed setting increased with age of the flower buds until a maximum some days before buds opened. After that, set decreased rapidly. Warmth shortened the period over which selfing was possible and shortened the period to the opening of the flowers. Most

  5. Phytase activity in brown rice during steeping and sprouting

    NARCIS (Netherlands)

    Ou, K.; Cheng, Y.; Xing, Y.; Lin, L.; Nout, M.J.R.; Liang, J.

    2011-01-01

    Phytase in brown rice will be activated and accumulated during seed germination. Changes of phytase activity in brown rice during two stages of germination (steeping and sprouting) affected by process conditions were studied. It was shown that steeping led to significant decrease of phytase activity

  6. Effect of sprouting and pre-gelatinization on the physicochemical ...

    African Journals Online (AJOL)

    Sprouted (96-hour) and pregelatinized (780C) sorghum grains were milled and blended with graded proportion of pigeon-pea and used in formulating flaked breakfast cereal. A commercial ready-to-serve breakfast cereal served as product control. The flour blends and formulated products were subjected to the ...

  7. 21 CFR 172.590 - Yeast-malt sprout extract.

    Science.gov (United States)

    2010-04-01

    ... additive may be used as a flavor enhancer in food at a level not in excess of that reasonably required to... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Yeast-malt sprout extract. 172.590 Section 172.590 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD...

  8. Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster

    Science.gov (United States)

    Baenas, Nieves; Piegholdt, Stefanie; Schloesser, Anke; Moreno, Diego A.; García-Viguera, Cristina; Rimbach, Gerald; Wagner, Anika E.

    2016-01-01

    We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus. PMID:26901196

  9. Photosynthate distribution patterns in cherrybark oak seedling sprouts

    Science.gov (United States)

    Brian Roy Lockhart; John D. Hodges; Emile S. Gardiner; Andrew W. Ezell

    2003-01-01

    Summary We used 14C tracers to determine photosynthate distribution in cherrybark oak (Quercus pagoda Raf.) seedling sprouts following release from competing mid-story vegetation. Fall acquisition of labeled photosynthates by seedlings followed expected source--sink patterns, with root and basal stem tissues...

  10. Effects of Growth Hormones on Sprouting and Rooting of Jatropha ...

    African Journals Online (AJOL)

    MICHAEL HORSFALL

    length, leaf number, and rooting parameters. Results showed that growth hormone application had no significant effect on survival and sprouting behaviour of J. curcas. The untreated cuttings (control) performed better than the hormone treated cuttings. However, in term of rooting behaviours, significant effect of treatment.

  11. Effects of magnetic fields pretreatment of mungbean seeds on sprout ...

    African Journals Online (AJOL)

    Tony

    2012-05-03

    May 3, 2012 ... control, magnetic field pretreatment caused an increase by 12% in the concentration of spouts protein, by 5.1% in the concentration of ... been reported by many researches. The first studies were conducted ... For protein extraction, 0.5 g mungbean sprouts (fresh weight) were homogenized at 0°C with 2.5 ...

  12. The current impact and potential of biotechnology to improve the capacity of orange-fleshed sweet potato to prevent vitamin A deficiency

    Science.gov (United States)

    Vitamin A deficiency is the leading cause of preventable blindness in the world and an important cause of premature death in young children and pregnant women. Billions of people get most of their vitamin A from plants that are rich in pro-vitamin A carotenoids such as beta-carotene. Orange-fleshe...

  13. Optimization of the composition of the powdered cereal sprouts mixtures

    Directory of Open Access Journals (Sweden)

    Tumbas-Šaponjac Vesna T.

    2016-01-01

    Full Text Available Sprouts of cereals have received significant attention as functional food due to their nutritional and functional value. Consumption of sprouts has become increasingly popular among people interested in improving and maintaining their health status by changing dietary habits. Cereal grains contain several classes of phytochemicals, i.e. phenolics, chlorophylls, and carotenoids. However, their nutritional and chemical profile is altered and improved during germination. The purpose of this study was to find the best ratio of the powdered wheat (WS, oat (OS and barley (BS sprouts for designing the cereal sprout mixture (CSM with the highest total phenolic content (TPh and antioxidant capacity (AC, using Simplex-Centroid experimental design and response surface methodology (RSM. Single- and multi-response optimizations showed that OS did not contribute to TPh or AC values of CSM and, therefore, was not included in any of the compositions of the optimized CSM. Single-response optimizations showed that the highest TPh was found for CSM containing 82% BS and 18% WS, while the best AC was found for pure BS. The predicted ratio of cereal sprout powders in CSM obtained by multi-response optimization was: 96% BS and 4% WS. This mixture possessed the highest predicted TPh and AC (372.32 mg GAE/100 g, 549.99 μmol TE/100 g, respectively, which was confirmed to be in accordance with the experimental values. Based on the results obtained in this study, a designed CSM is proposed as a convenient ingredient of functional food products, dietary supplements and nutraceuticals.

  14. Microbiological aspects of storage of irradiated potatoes and wheat

    International Nuclear Information System (INIS)

    Nadkarni, G.B.

    1978-01-01

    Radiation provides an effective means for extending the post-harvest storage life of perishable foods. Based on extensive studies over the past two decades in India and abroad, WHO have recently recognised potatoes and wheat as 'unconditionally' safe for human consumption alongwith several other items of food. In potatoes exposed to 10 Krad of gamma-irradiation, sprouting and tuber moth infestation were completely eliminated, irrespective of the storage temperature. The ambient temperatures could not however control the microbial rotting of the tubers. Microbiological studies have indicated that spoilage at temperatures above 15 0 C are due to Erwinia spp. while dry rotting below 10 0 C could be attributed to Micrococcus spp. The tubers were not amenable to fungal attack by organisms of Aspergillus spp. during storage due to phenolic compounds which were otherwise labile to heating. The studies with artificial loading of wheat with conidia of Aspergillus flavus showed a correlation between growth and relative humidity (RH) which determined the moisture content in the grain. Aflatoxin production could occur only above a critical level (13%) of grain moisture at RH value over 90% at 28 0 +- 2 0 C. The toxin produced in irradiated grains seemed to be lower than in the unirradiated controls, indicating that radiation treatment did not cause any alterations in wheat which could otherwise promote aflatoxin producing potential. (author)

  15. Effect of Thiourea on Yield and Quality of Potato (Solanum tuberosum L.

    Directory of Open Access Journals (Sweden)

    Mani, F.

    2013-02-01

    Full Text Available A field experiment was carried out to study the effect of five concentrations (0, 250, 500, 750 and 1000 mM of thiourea application on mother tubers on yield and quality attributes of harvested potato, variety Spunta in the region of Chott-Mariem in Tunisia. Treated tubers (Ш = 50 mm were planted in field after breaking dormancy at a spacing of 80 cm x 30 cm according to completely randomized design (CRD in three replications. Quality attributes of potato tubers especially fresh matter and dry matter of plant, total reducing sugars in leaves, tuber yield, number and diameter of tubers per plant and number of sprouts per tuber was measured. Soluble proteins and starch content in potato tubers were also quantified.In general we noted that application of thiourea showed significant influence on yield and on quality of tubers comparing to control. Maximum tuber yield per plant, maximum number of tubers per plant and maximum starch content was recorded with 250 mM of thiourea. Moreover, highest dry matter of potato plant was found also at this concentration. While increasing dose of thiourea thereafter it showed slight significant improvement and do not affect significantly the diameter of tubers comparing to control.

  16. Skin quality in red potatoes

    Science.gov (United States)

    Attractive appearance is a highly desirable characteristic of fresh market red-skinned potatoes. The ideal red potato has a rich, uniform, deep red color. Color fading, netting, browning, and discoloration caused by skinning and disease decrease marketability and may reduce profits to growers and pa...

  17. Acrylamide in processed potato products

    Science.gov (United States)

    Trace amounts of acrylamide are found in many foods cooked at high temperatures. Acrylamide in processed potato products is formed from reducing sugars and asparagine and is a product of the Maillard reaction. Processed potato products including fries and chips are relatively high in acrylamide comp...

  18. Growth and quality of soybean sprouts (Glycine max L. Merrill) as affected by gamma irradiation

    International Nuclear Information System (INIS)

    Yun, Juan; Li, Xihong; Fan, Xuetong; Li, Weili; Jiang, Yuqian

    2013-01-01

    In this study, soybean seeds and sprouts (Glycine max L. Merrill) were exposed to radiation doses up to 3.0 kGy. The irradiated and non-irradiated seeds were germinated, and then germination rate, sprouts length, vitamin C content, antioxidants and visual and olfactory quality were determined after irradiation. Results indicated that there was no significant difference in the germination rate and sprouts length between the control and 0.3 kGy treated soybeans, however, the reductions in sprouts length of the 1.0 kGy and 3.0 kGy treated samples were quite significant with reductions of 20.4% and 58.8%, respectively. Irradiated sprouts had similar visual and olfactory quality as the non-irradiated one. Therefore, irradiation of seeds alone would have limited value in terms of commercial use due to reduced germination and length of sprouts. However, irradiation of sprouts at doses up to 3.0 kGy was feasible to enhance microbial safety of sprouts. - Highlights: ► Investigated the germination rate and the sprouts length after irradiation. ► Indicated the effect of irradiation on the antioxidants of the soybean sprouts. ► Evaluated the visual and olfactory quality of irradiated samples.

  19. Transgenic approaches in potato: effects on glycoalkaloids levels

    African Journals Online (AJOL)

    Sayyar

    2013-02-20

    Feb 20, 2013 ... unwanted characters in genetically engineered crop plants. In this context, the subject of ... Key words: Unintended effects, transgenic potato, glycoalkaloids, food safety, environmental stress. INTRODUCTION. Genetic ..... infection, prevention of human colon and liver cancer cells, enhancement of general ...

  20. Electroacupuncture at ST36-ST37 and at Ear Ameliorates Hippocampal Mossy Fiber Sprouting in Kainic Acid-Induced Epileptic Seizure Rats

    Directory of Open Access Journals (Sweden)

    Chung-Hsiang Liu

    2014-01-01

    Full Text Available Our previous study showed that mossy fiber sprouting can occur in the hippocampus region in rats 6 wk after kainic acid-induced epileptic seizure, and this mossy fiber sprouting can facilitate epileptogenesis. Transcutaneous auricular vagal nerve stimulation (VNS, which is similar to cervical VNS, can reduce the occurrence of epileptic seizure in intractable epilepsy patients. Greater parasympathetic nerve activity can be caused by 2 Hz electroacupuncture (EA. Therefore, we investigated the effect of 2 Hz EA at ST-36-ST37 and at the ear on mossy fiber sprouting in kainic-treated Sprague-Dawley rats. The results indicated that applying 2 Hz EA at ST36-ST37 and at the ear for 3 d per week over 6 consecutive weeks can ameliorate mossy fiber sprouting in the hippocampus region of rats. These results indicated that applying 2 Hz EA at ST36-ST37 and at the ear might be beneficial for the treatment and prevention of epilepsy in humans.

  1. ESTIMATION OF BIOMASS COMMERCIAL SPROUTS OF Ilex paraguariensis A.ST.-HIL

    Directory of Open Access Journals (Sweden)

    Elisabete Vuaden

    2009-10-01

    Full Text Available This study aimed at developing some models that allow estimating the biomass of commercial green shoots of Ilex paraguariensis after pruning, at 10 years-old. In September 2007, 40 Ilex paraguariensis were pruned. One year after the first pruning, in 2008, they were evaluated dendrometrically and their biomass was determined from the commercial harvest of 16 individuals. To the others, the commercial biomass was estimated by the sum of the biomass of shoots.  The result obtained is that the biomass of commercial sprouts can be estimated as a function of the length of the rolls sprouting, with specific models for sprouts short, simple and compound average sprouts and long sprouts compounds. The models used to estimate the biomass of commercial sprouts using the length sum rolls and rolls of the length as independent variables underestimate the values of biomass with a margin of error of only 2.6%.

  2. Effect of low-temperature storage, gamma irradiation and iso-propyl-N-(3-chlorophenyl carbamate) treatment on the processing quality of potatoes

    International Nuclear Information System (INIS)

    Liu, M.S.; Chen, R.Y.; Tsai, M.J.

    1990-01-01

    In Taiwan commercial storage of potatoes (CV Kennebec, Wu-Foon, Cardinal) at 2°C. 85% RH, can supply the fresh market all year round. However, reducing sugar and total sugar which accumulate in the tubers will reduce the processing quality of potatoes. Reconditioning of tubers from cold stores was undertaken at ambient temperature (20°C) for up to 6 weeks. The optimum temperature for Kennebec and Cardinal reconditioning ranged from 20 to 25°C. However, after storage at 2°C for a long period, the sugar content of potato tubers cannot be reduced to a satisfactory level. When newly harvested potatoes were pretreated with CIPC (300–500 μg litre −1 ) and stored at 10°C, 80 % RH, sprouting was inhibited effectively. The quality of potatoes was retained well and reducing sugar remained at a low level for up to 8 months. MENA was found to be ineffective. Gamma irradiation of potatoes at 0.175-0 20·0-10–0.125 and 0.125 kGy for Cardinal, Kennebec and Wu-Foon respectively was found to be effective in controlling the sprouting of tubers stored at 10°C, 80 % RH, for 9 months. A rapid increase in sugar content was found in irradiated Cardinal and Wu-Foon after storage for 7 months, while the reducing sugar in irradiated Kennebec was still below 0°2 % after 8 month's storage. Gamma irradiation had no adverse effect on the ascorbic acid content, and higher firmness retention was obtained in irradiated tubers throughout storage at 10°C

  3. POTENCY OF MUNG BEAN SPROUT AS ENZYME SOURCE (α-AMILASE

    Directory of Open Access Journals (Sweden)

    Suarni Suarni

    2010-06-01

    Full Text Available Mung bean sprouts contain enzyme of α-amylase. A research on the effect of the sprout age and sprout varieties to the α-amylase activity and the protein level has been carried out in Laboratorium Bioproses BB Pascapanen Bogor using  Full Factorial Random Design with two factorials (1 sprout age; 1, 2, 3, 4, and 5 days as well as (2 varieties of mung bean; Kenari, Bhakti and Parkit.  Parameters observed were water and protein content of sprout, pH, activities of α-amylase, and dissolved protein in the enzyme extract. Results showed that the optimum temperature of α-amylase was 30 ºC, the highest protein level of sprout and the highest activity of α-amylase were given by the sprout of Bhakti at the age of three days. The water content in sprout was 65.23%, the protein level was 12.93 %, the dissolved protein in the enzyme extract was 2.88743 mg/mL, pH was 5.45, and the activity of enzyme was 4.09 Unit/mL. The potency of enzyme found in mung bean can be utilized in industries processing materials having high starch content, such as maize flour.   Keywords;  sprout of mung bean, activity of α-amylase, protein

  4. Preferentially enhancing anti-cancer isothiocyanates over glucosinolates in broccoli sprouts: How NaCl and salicylic acid affect their formation.

    Science.gov (United States)

    Esfandiari, Azadeh; Saei, Ali; McKenzie, Marian J; Matich, Adam J; Babalar, Mesbah; Hunter, Donald A

    2017-06-01

    Broccoli (Brassica oleracea L. var. italica) sprouts contain glucosinolates (GLs) that when hydrolysed yield health promoting isothiocyanates such as sulforaphane (SF). SF content can be increased by salt (NaCl) stress, although high salt concentrations negatively impact plant growth. Salicylic acid (SA) treatments can attenuate the negative effects of salt on growth. To test whether sprout isothiocyanate content could be elevated without sprout growth being compromised, broccoli seed were germinated and grown for seven days in salt (0, 80 and 160 mM) alone and in combination with 100 μM SA. Increasing concentrations of salt lowered transcript accumulation of GL biosynthetic genes which was reflected in lowered content of Gluconapin, 4-methoxyglucobrassicin and neoglucobrassicin glucosinolates. Other glucosinolates such as glucoraphanin did not alter significantly. Salt (160 mM) increased transcript abundance of the GL hydrolytic gene MYROSINASE (BoMYO) and its cofactor EPITHIOSPECIFIER MODIFIER1 (BoESM1) whose encoded product directs MYROSINASE to produce isothiocyanate rather than nitrile forms. SF content was increased 6-fold by the 160 mM salt treatment, but the salt treatment reduced percentage seed germination, slowed seed germination, and reduced sprout hypocotyl elongation. This growth inhibition was prevented if 100 μM SA was included with the salt treatment. These findings suggest that the increase in SF production by salt occurs in part because of increased transcript abundance of genes in the hydrolytic pathway, which occurs independently of the negative impact of salt on sprout growth. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  5. Sweet potato for biomass

    Energy Technology Data Exchange (ETDEWEB)

    Dangler, J.M.; Locascio, S.J.; Halsey, L.H.

    1984-01-01

    Field experiments were conducted in 1980 and 1981 to determine the root and plant top yield of sweet potato (Ipomoea batatas L. (Lam)) grown on a sandy soil. Cultivars 'GaTG-3', 'Morado', 'Rojo Blanco', 'Travis' and 'White Star' were evaluated at 2 harvest times. Mean starch yields from 'GaTG-3' at 105-115 days (7.2 t/hectare) and at 210-230 days (9.6 ton/hectare) during two seasons were higher than from the other cultivars. With an increase in the growth period from 105-115 to 210-230 days the means starch yield increased from 4.6 to 7.3 t/hectare but the starch concentration of all cultivars decreased significantly during the same period.

  6. Structure of potato starch

    DEFF Research Database (Denmark)

    Bertoft, Eric; Blennow, Andreas

    2016-01-01

    Potato starch granules consist primarily of two tightly packed polysaccharides, amylose and amylopectin. Amylose, which amount for 20-30%, is the principal linear component, but a fraction is in fact slightly branched. Amylopectin is typically the major component and is extensively branched......, containing short chains with an average length of 22-25 glucosyl residues. The branching pattern is not well known, but branch point clustering guides chains to determine the overall starch granule architecture and starch functionality. The clusters consist of 5-10 grouped short chains, which...... are interconnected by long chains with more than 36 residues. The clusters consist of still smaller, very tightly branched building blocks. The clusters direct the semicrystalline structures found inside the starch granules. The crystals, which are ~5.2. nm thick, contain double helices formed from the external...

  7. Feasibility of radiation processing for post-harvest storage of potatoes under tropical conditions

    International Nuclear Information System (INIS)

    Thomas, P.; Srirangarajan, A.N.; Padwal-Desai, S.R.; Ghanekar, A.S.; Shirsat, S.G.; Pendharkar, M.B.; Nair, P.M.; Nadkarni, G.B.

    1978-01-01

    Storage changes after gamma irradiation (10krad) of five commercial varieties of potatoes, Kufri Chandramukhi, K. Kuber, K. Alankar, K. Sheetman and K. Sinduri, grown in India were ascertained to arrive at optimal storage conditions. The radiation treatment could completely inhibit sprouting in all the varieties, irrespective of storage temperature, in addition to eliminating the tuber moth Pthorimaea operculella (Zeller). The temperature-dependent total losses could be attributed to microbial rotting and dehydration. The organisms causing soft rot at temperatures above 15 0 C belonged to Erwinia spp., while the rot caused below 10 0 C was essentially due to Micrococcus spp. Irradiation followed by storage at cool temperatures (10-15 0 C) offers an alternative method, comparable with conventional refrigerated (2-4 0 C) storage. The irradiated potatoes were not amenable to the attack of toxigenic fungi Aspergillus flavus and A. parasiticus during storage. This could be attributed to phenolic compounds present in the tubers. Phenylalanine ammonia lyase (PAL), involved in the metabolism of phenolic compounds, showed enhancement in the irradiated potatoes. The extent of radiation-induced activation of PAL is suggested as a biochemical parameter for identifying irradiated tubers. (author)

  8. EFFECT OF SOME HERBICIDES APPLICATION ON NITRATES (V CONTENT IN POTATO TUBERS

    Directory of Open Access Journals (Sweden)

    Krystyna Zarzecka

    2016-07-01

    Full Text Available A field experiment was carried in the Agricultural Experimental Station Zawady (52°03’ N; 22°33’ E,owned by the Siedlce University of Natural Sciences and Humanities out in 2005-2007. The research was designed as a two factors randomized block with three replicates. Factors examined in the experiment included two potato varieties - Irga and Balbina and four weed control methods combined mechanical and chemical for herbicides application: Plateen 41,5 WG (metribuzin + flufenacet, Racer 250 EC (fluorochloridone, Sencor 70 WG (metribuzin, and control object – mechanical weeding before and after potato sprouting. The study was designed to test the influence of the weed control methods on nitrates content in consumption potato tubers. The nitrates (V content depended on the weed control methods, varieties and weather conditions throughout the growing season. The highest nitrates (V content was determined when weeds were controlled mechanically and chemically using Sencor 70 WG. The study results demonstrated that Balbina had a higher concentration of nitrates (V compared with Irga.

  9. Development of epicormic sprouts in Sitka spruce following thinning and pruning in south-east Alaska.

    Science.gov (United States)

    Robert L. Deal; R. James Barbour; Michael H. McClellan; Dean L. Parry

    2003-01-01

    The frequency and size of epicormic sprouts in Sitka spruce (Picea sitchensis (Bong.) Carr.) were assessed in five 23-29 year-old mixed Sitka spruce-western hemlock (Tsuga heterophylla (Raf.) Sarg.) stands that were uniformly thinned and pruned to 2.4, 3.7 and 5.2 m lift heights. Six to nine years after treatment sprouts were...

  10. Sprouting characteristics and associated changes in nutritional composition of cowpea (Vigna unguiculata).

    Science.gov (United States)

    Devi, Chingakham Basanti; Kushwaha, Archana; Kumar, Anil

    2015-10-01

    Cowpea (Vigna unguiculata), is an important arid legume with a good source of energy, protein, vitamins, minerals and dietary fibre. Sprouting of legumes enhances the bioavailability and digestibility of nutrients and therefore plays an important role in human nutrition. Improved varieties of grain cowpea viz. Pant Lobia-1 (PL-1) and Pant Lobia-2 (PL-2) and Pant Lobia-3 (PL-3) were examined for sprouting characteristics and associated changes in nutritional quality. Soaking time, sprouting time and sprouting temperature combinations for desirable sprout length of ¼ to ½ inch for cowpea seed samples were standardized. All the observations were taken in triplicate except soaking time, where six observations were taken in a completely randomized design of three treatments. Results revealed that optimum soaking time of PL-1 and PL-2 seed was 3 h whereas PL-3 required 9 h. Sprouting period of 24 h at 25 °C was found to be desirable for obtaining good sprouts. Significant improvement in nutritional quality was observed after sprouting at 25 °C for 24 h; protein increased by 9-12 %, vitamin C increased by 4-38 times, phytic acid decreased by 4-16 times, trypsin inhibitor activity decreased by 28-55 % along with an increase of 8-20 % in in-vitro protein digestibility.

  11. Kinetics of Sulforaphane in Mice after Consumption of Sulforaphane-Enriched Broccoli Sprout Preparation

    Science.gov (United States)

    Li, Yanyan; Zhang, Tao; Li, Xiaoqin; Zou, Peng; Schwartz, Steven J.; Sun, Duxin

    2013-01-01

    Scope Sulforaphane is a natural isothiocyanate in broccoli sprouts with cancer chemopreventive activity. This study is aimed to to use different methods to develop broccoli sprout preparations to compare their ability to deliver sulforaphane to the mice and to evaluate the kinetics and biodistribution of sulforaphane. Methods and Results The sulforaphane-enriched sprout preparation generated by two-step procedure (quick-steaming followed by myrosinase treatment) contained the highest level of sulforaphane, which was 11 and 5 times higher than the freeze-dried fresh broccoli sprouts and the quick-steamed, freeze-dried broccoli sprouts, respectively. After oral administration of 2.5 mg/g body weight of the broccoli sprout preparations, sulforaphane was quickly absorbed and distributed throughout the tissues. The sulforaphane-rich preparation resulted in the highest exposure, with peak plasma sulforaphane concentration of 337 ng/ml, which is 6.0 times and 2.6 times higher compared to the other two preparations. A whole body physiologically-based pharmacokinetic model (developed with ADAPT 5 software) suggests that distribution of sulforaphane is perfusion-limited in all organs. Conclusion This study provides a broccoli sprout preparation that can serve as a good source of sulforaphane, and the model can be utilized to guide the dose design for the use of broccoli sprout preparation in chemoprevention. PMID:23929742

  12. Effects of yeast polysaccharide on growth and flavonoid accumulation in Fagopyrum tataricum sprout cultures.

    Science.gov (United States)

    Zhao, Gang; Zhao, Jianglin; Peng, Lianxin; Zou, Liang; Wang, Jingbo; Zhong, Lingyun; Xiang, Dabing

    2012-09-25

    The purpose of this study was to investigate the effects of yeast polysaccharide (YPS) on growth and flavonoid accumulation in sprout cultures of Fagopyrum tataricum (tartary buckwheat). Without obvious change in the appearance of the sprouts, the exogenous YPS notably stimulated the production of functional metabolites in F. tataricum sprouts, and the stimulation effect was concentration-dependent. With 400 mg/L of YPS applied to the sprout cultures on day 6, the total rutin and quercentin content was effectively increased to 42.8 mg/gdw, or about 1.4-fold in comparison with the control of 31.2 mg/gdw. Feeding with 800 mg/L of YPS on day 9, the sprouts biomass was increased by about 8% compared to the control culture (0.99 gdw/100 sprouts versus 0.92 gdw/100 sprouts). Moreover, the present study revealed that the accumulation of these bioactive metabolites resulted from the stimulation of the phenylpropanoid pathway by YPS treatment. It could be an effective strategy for improving the functional quality of the F. tataricum sprouts provided with YPS.

  13. Evaluation of oak stump sprouting in the Missouri Ozarks ten years after harvest

    Science.gov (United States)

    Randy G. Jensen; Daniel C. Dey

    2008-01-01

    Managers in Missouri often want to manage forests to retain oak in the future. Oaks are valuable for timber and many wildlife species depend on acorns. Large advance reproduction and stump sprouts are the most competitive sources of regeneration. It is well known that oak stump sprouts contribute to future stands in even-age clearcuts in the Missouri Ozarks, but there...

  14. Prevention

    Science.gov (United States)

    ... Error processing SSI file About Heart Disease & Stroke Prevention Heart disease and stroke are an epidemic in ... secondhand smoke. Barriers to Effective Heart Disease & Stroke Prevention Many people with key risk factors for heart ...

  15. Preliminary study on the effect of cobalt-60 gamma radiation on the conservation of potato tubers for consumption (Solanum tuberosum l.), during the storage period

    International Nuclear Information System (INIS)

    Montepeque Roldan, R.

    1984-03-01

    The object of the thesis is the study of the inhibiting effect of the sprouting of potato tubers, variety Loman, after Cobalt-60 gamma irradiation (with doses of 4, 6, 8, 10 and 12 Krads) as well as after treatment with the chemical inhibitor Isopropyl-N-Phenyl Carbonate (IPC). These treatments were applied to different groups of tubers, after 15 and 45 days from harvesting, stored under two illumination conditions: darkness (closed room), and indirect light (straw hut). The number, length and thickness of the sprouts, as well as the weight, humidity contents and infection of the tubers were recorded twice a month. A random 2x2x7x4 multifactorial design, with 3 repetitions, was used for the analysis and interpretation of the results. Variance and multiple regression analysis, as well as Tukey tests were performed on the IBM/370 computer of the San Carlos University (Guatemala). It was found that both the 4 and 6 Krad doses and the IPC treatments have no effect on the sprouting inhibition. The irradiation with the 8, 10, and 12 Krad doses inhibited the sprouting in an irreversible way, with insignificant loss of weight and moisture, regardless of the irradiation epoch and light condition. However, the tubers stored in darkness, showed ideal characteristics for consumption, and the 8 Krad dose, applied at 15 days after harvesting is concluded to be the best

  16. Storage properties of irradiated potatoes and onions. Part of a coordinated programme on the Asian Regional Cooperative Project on Food Irradiation

    International Nuclear Information System (INIS)

    Hayashi, T.

    1984-12-01

    Quality changes with regard to browning of inner buds and sprouting of irradiated onions variety Kitamiki and Ohotsuku were investigated. One ton of each variety was irradiated with either 38.1 or 65.2 Gy at the Commercial Potato Irradiator in Shihoro and stored either at 0 deg. C or 5 deg. C. After 6 months of storage, they were taken out to be stored at room temperature. It was observed that green inner buds developed in most unirradiated onions which showed no external sprouting irrespective of storage temperature. Irradiated onions show no external or internal sprouting but exhibited browning of small inner buds. Unirradiated onions rooted slightly during storage at low temperature. None of the irradiated samples showed rooting. Neither of the samples spoiled significantly during storage. It was concluded that storage at room temperature accelerated sprouting and development of green inner buds of unirradiated onions and browning of small inner buds in irradiated ones. During off-seasons, only irradiated onions stored at low temperatures have qualities of commercial significance

  17. Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison

    DEFF Research Database (Denmark)

    Li, Chengdao; Ni, Peixiang; Francki, Michael

    2004-01-01

    Pre-harvest sprouting results in significant economic loss for the grain industry around the world. Lack of adequate seed dormancy is the major reason for pre-harvest sprouting in the field under wet weather conditions. Although this trait is governed by multiple genes it is also highly heritable....... A major QTL controlling both pre-harvest sprouting and seed dormancy has been identified on the long arm of barley chromosome 5H, and it explains over 70% of the phenotypic variation. Comparative genomics approaches among barley, wheat and rice were used to identify candidate gene(s) controlling seed...... dormancy and hence one aspect of pre-harvest sprouting. The barley seed dormancy/pre-harvest sprouting QTL was located in a region that showed good synteny with the terminal end of the long arm of rice chromosome 3. The rice DNA sequences were annotated and a gene encoding GA20-oxidase was identified...

  18. Potato virus Y induced changes in the gene expression of potato (Solanum tuberosum L.)

    NARCIS (Netherlands)

    Pompe-Novak, M.; Gruden, K.; Baebler, P.; Krecic-Stress, H.; Kovac, M.; Jongsma, M.A.; Ravnikar, M.

    2005-01-01

    The tuber necrotic strain of Potato virus Y (PVYNTN) causes potato tuber necrotic ringspot disease in sensitive potato cultivars. Gene expression in the disease response of the susceptible potato (Solanum tuberosum L.) cultivar Igor was investigated at different times after infection, using

  19. Genetic fingerprinting of potato varieties from the Northwest Potato Variety Development Program

    Science.gov (United States)

    The Northwest Potato Variety Development Program using conventional breeding has successfully released more than 40 improved varieties of potato since its inception in 1983. Potato breeders rely primarily on morphological and phenotypic data for selection and breeding of potato cultivars. With the a...

  20. ENZYME RESISTANCE OF GENETICALLY MODIFIED STARCH POTATOES

    Directory of Open Access Journals (Sweden)

    A. Sh. Mannapova

    2015-01-01

    Full Text Available Here in this article the justification of expediency of enzyme resistant starch use in therapeutic food products is presented . Enzyme resistant starch is capable to resist to enzymatic hydrolysis in a small intestine of a person, has a low glycemic index, leads to decrease of postprandial concentration of glucose, cholesterol, triglycerides in blood and insulin reaction, to improvement of sensitivity of all organism to insulin, to increase in sense of fulness and to reduction of adjournment of fats. Resistant starch makes bifidogenшс impact on microflora of a intestine of the person, leads to increase of a quantity of lactobacillus and bifidobacterium and to increased production of butyric acid in a large intestine. In this regard the enzyme resistant starch is an important component in food for prevention and curing of human diseases such as diabetes, obesity, colitis, a cancer of large and direct intestine. One method is specified by authors for imitation of starch digestion in a human body. This method is based on the definition of an enzyme resistance of starch in vitro by its hydrolysis to glucose with application of a glucoamylase and digestive enzyme preparation Pancreatin. This method is used in researches of an enzyme resistance of starch, of genetically modified potato, high amylose corn starch Hi-Maize 1043 and HYLON VII (National Starch Food Innovation, USA, amylopectin and amylose. It is shown that the enzyme resistance of the starch emitted from genetically modified potatoes conforms to the enzyme resistance of the high amylose corn starch “Hi-Maize 1043 and HYLON VII starch”, (National Starch Food Innovation, the USA relating to the II type of enzyme resistant starch. It is established that amylopectin doesn't have the enzyme resistant properties. The results of researches are presented. They allow us to make the following conclusion: amylose in comparison with amylopectin possesses higher enzyme resistance and gives to

  1. Wholesomeness of irradiated foods, especially potatoes, in Japan

    International Nuclear Information System (INIS)

    Furuya, Tsuyoshi

    1998-01-01

    Food irradiation was established under the auspices of the Japan Atomic Energy Commission. In 1967, studies on the efficacy of food irradiation, irradiation techniques and wholesomeness of irradiated foods were begun on potatoes and 6 other items (onions, rice, wheat, vienna sausage, fish-paste products, and mandarin orange) by a project team consisting of scientists from various broad academic fields under the sponsorship of the Science and Technology Agency. Wholesomeness of irradiated foods generally includes the following three points. The first one is the toxicity consisting of chronic toxicity test which predicts the effect when food is continuously ingested for long time, e.g., carcinogenicity test, teratogenicity test which predicts the effect of reproduction and generation for multigeneration, and mutagenicity test which detects the cytogenic possibility. The second is the nutritional adequacy as evaluated by effect on growth, physiological function and nutritional contents. The last one is microbiological safety concerning to microorganic resistance and tolerance by irradiation. In 1972, on the basis of the reports on toxicological aspects that we mainly carried out, nutritional and other aspects, 0.15 kGy level for sprout inhibition of potato which was the first practical use for food irradiation in Japan was permitted by Food Sanitation Act. The safety of food contaminants and food additives, its object for use the same as food irradiation, is confirmed by toxicity test employed experimental animals. Limitation of food additives and residual limitation of food contaminants are decided by the results in toxicity test. And these insure humansafety. It is important and necessary to choose the irradiation or chemicals for food in view of human health. (author)

  2. Flow cytometry for rapid detection of Salmonella spp. in seed sprouts

    Directory of Open Access Journals (Sweden)

    Bledar Bisha

    2014-12-01

    Full Text Available Seed sprouts (alfalfa, mung bean, radish, etc. have been implicated in several recent national and international outbreaks of salmonellosis. Conditions used for sprouting are also conducive to the growth of Salmonella. As a result, this pathogen can quickly grow to very high cell densities during sprouting without any detectable organoleptic impact. Seed sprouts typically also support heavy growth (~108 CFU g−1 of a heterogeneous microbiota consisting of various bacterial, yeast, and mold species, often dominated by non-pathogenic members of the family Enterobacteriaceae. This heavy background may present challenges to the detection of Salmonella, especially if this pathogen is present in relatively low numbers. We combined DNA-based fluorescence in situ hybridization (FISH with flow cytometry (FCM for the rapid molecular detection of Salmonella enterica ser. Typhimurium in artificially contaminated alfalfa and other seed sprouts. Components of the assay included a set of cooperatively binding probes, a chemical blocking treatment intended to reduce non-specific background, and sample concentration via tangential flow filtration (TFF. We were able to detect S. Typhimurium in sprout wash at levels as low as 103 CFU ml−1 sprout wash (104 CFU g−1 sprouts against high microbial backgrounds (~108 CFU g−1 sprouts. Hybridization times were typically 30 min, with additional washing, but we ultimately found that S. Typhimurium could be readily detected using hybridization times as short as 2 min, without a wash step. These results clearly demonstrate the potential of combined DNA-FISH and FCM for rapid detection of Salmonella in this challenging food matrix and provide industry with a useful tool for compliance with sprout production standards proposed in the Food Safety Modernization Act (FSMA.

  3. Acrylamide Mitigation Procedures in Fried Potatoes

    DEFF Research Database (Denmark)

    Pedreschi, Franco; Kaack, Karl; Granby, Kit

    2008-01-01

    Acrylamide diminishing in potato slices and strips was studied in relation to frying temperature and some pre-treatments. Potato slices (Tivoli variety, diameter 37 mm, width: 2.2 mm) were fried at 150, 170 and 190 degrees C until reaching moisture contents of similar to 1.8 percent Prior to frying...... strips (0.8 x 0.8 x 5 cm) of Bintje variety were fried at 150, 170 and 190 degrees C until reaching moisture contents of similar to 40 percent. Prior to frying, potato strips were treated in similar ways to potato slices. Glucose and asparagine contents were determined in potato slices and strips before...... frying, whereas acrylamide content was determined in the fried potato chips and French fries French fries. Blanching reduced in potato chips on average 76 percent and 68 percent of the glucose and asparagine content compared to the control. Potato slices blanched at 50 degrees C for 70 minutes...

  4. Characterization of Digestion Resistance Sweet Potato Starch ...

    African Journals Online (AJOL)

    Purpose: To analyze the physicochemical properties and in vitro digestibility of sweet potato starchphosphodiester prepared using sodium trimetaphosphate. Methods: The physicochemical properties of sweet potato starch phosphodiester were analyzed by using infrared spectrometry (IR), differential scanning calorimetry ...

  5. Characterization of Digestion Resistance Sweet Potato Starch ...

    African Journals Online (AJOL)

    HP

    Purpose: To analyze the physicochemical properties and in vitro digestibility of sweet potato starch phosphodiester prepared using sodium trimetaphosphate. Methods: The physicochemical properties of sweet potato starch phosphodiester were analyzed by using infrared spectrometry (IR), differential scanning calorimetry ...

  6. Chemical, sensory and rheological properties of porridges from processed sorghum (Sorghum bicolor), bambara groundnut (Vigna subterranea L. Verdc) and sweet potato (Ipomoea batatas) flours.

    Science.gov (United States)

    Nnam, N M

    2001-01-01

    The chemical, sensory and rheological properties of porridges made from blends of sprouted sorghum, bambara groundnuts and fermented sweet potatoes were examined. Sorghum and bambara groundnuts were sprouted for 48 h while sweet potatoes were fermented for the same period. Blends were formulated from the processed ingredients in the ratio of 60:40:0, 57:42:1, 55:44:1 and 52:46:2 (protein basis) of sorghum, bambara groundnuts and sweet potatoes. Porridges were prepared from the composite flours and the traditional sorghum complementary food. Standard assay methods were used to evaluate the flours for nutrient composition. The porridges were also tested for sensory properties and viscosity. Processing increased the levels of most of the nutrients evaluated. Relative to the sorghum traditional complementary food, the composite flours had higher levels of lipids, protein, ash, crude fiber and minerals (p < 0.05). The porridges from the composite flours were generally liked slightly by the panelists and were about seven times less viscous than the porridge from the traditional sorghum complementary food. Use of the composite flours, particularly the 52:46:2 blend, as a traditional complementary food should be encouraged in Nigeria especially with the increasing cost of commercial complementary foods.

  7. Potato Organic Farming in Batu City, Indonesia

    OpenAIRE

    Sugiarto, Sugiarto; Sulistiono, Rudi; Sudiarso, Sudiarso; Soemarno, Soemarno

    2013-01-01

    Organic potato cultivation was an effort to improve declining quality of potato agroecosystems and to preserve soil fertility. This study was conducted to analyze pattern of farming system and land management on the area of organic potatoes in Batu city. Research was conducted by the survey method, respondents were determined by the method of stratified cluster sampling. Farmer respondents were experienced organic potato farmer and as "expert leaders". Results show that the mindset of farmer...

  8. Globalization of the Frozen Potato Industry

    OpenAIRE

    Makki, Shiva S.; Plummer, Charles

    2005-01-01

    This paper examines the globalization of the frozen potato industry, including the expansion of production and processing of potato products in the global market place, and future prospects for the industry. Driven largely by the rising popularity of Western style cuisine, frozen french fries and other frozen potato products have become global commodities generating billions of dollars in revenue worldwide. This study finds that with markets for frozen potato products in the United States and...

  9. Glycoalkaloids in potatoes: Content of glycoalkaloids in potatoes for consumption

    DEFF Research Database (Denmark)

    Knuthsen, Pia; Jensen, Udo; Schmidt, Bjørn

    2009-01-01

    mg/kg. Thus the aim of this study was to make a survey of the distribution and contents of TGA in potatoes on the Danish market, including many different and relevant varieties during a 6-year period. A total of 386 samples of potato tubers were analysed for α-solanine and α-chaconine by extraction...... with acetic acid and determination by RP-HPLC with UV-detection at 202 nm. The results not only confirmed that contents above 100 mg TGA/kg in potato tubers frequently occurred in some years, but also showed the possibility of finding lower contents in the same varieties other years. This led to the cautious...

  10. Minituber production potential of selected Potato ( Solanum ...

    African Journals Online (AJOL)

    Potato farmers in Malawi and other developing countries lack healthy and quality potato seed. This is mainly due to limited seed multiplication programmes to provide farmers with clean potato seed. A study to evaluate minituber production potential for selected genotypes in different media types would assist in planning for ...

  11. Climate change impact on global potato production

    NARCIS (Netherlands)

    Raymundo, Rubí; Asseng, Senthold; Robertson, Richard; Petsakos, Athanasios; Hoogenboom, Gerrit; Quiroz, Roberto; Hareau, Guy; Wolf, Joost

    2017-01-01

    Potato is the most important non-grain crop in the world. Therefore, understanding the potential impacts of climate change on potato production is critical for future global food security. The SUBSTOR-Potato model was recently evaluated across a wide range of growing conditions, and improvements

  12. Sweet potato (Ipomoea batatas) attenuates diet-induced aortic stiffening independent of changes in body composition.

    Science.gov (United States)

    Garner, Tyler; Ouyang, An; Berrones, Adam J; Campbell, Marilyn S; Du, Bing; Fleenor, Bradley S

    2017-08-01

    We hypothesized a sweet potato intervention would prevent high-fat (HF) diet-induced aortic stiffness, which would be associated with decreased arterial oxidative stress and increased mitochondrial uncoupling. Young (8-week old) C57BL/6J mice were randomly divided into 4 groups: low fat (LF; 10% fat), HF (60% fat), low-fat sweet potato (LFSP; 10% fat containing 260.3 μg/kcal sweet potato), or high-fat sweet potato diet (HFSP; 60% fat containing 260.3 μg/kcal sweet potato) for 16 weeks. Compared with LF and LFSP, HF- and HFSP-fed mice had increased body mass and percent fat mass with lower percent lean mass (all, P Sweet potato intervention did not influence body composition (all, P > 0.05). Arterial stiffness, assessed by aortic pulse wave velocity and ex vivo mechanical testing of the elastin region elastic modulus (EEM) was greater in HF compared with LF and HFSP animals (all, P sweet potato attenuates diet-induced aortic stiffness independent of body mass and composition, which is associated with a normalization of arterial oxidative stress possibly due to mitochondrial uncoupling.

  13. Shelf-life of prepeeled potato cultivated, stored, and processed by various methods.

    Science.gov (United States)

    Ahvenainen, R T; Hurme, E U; Hägg, M; Skyttä, E H; Laurila, E K

    1998-05-01

    The effects of cultivation conditions, winter storage, peeling method, browning prevention chemicals replacing sodium bisulfite, and packing methods on the sensory, nutritional and microbiological quality of pre-peeled potato were examined. Two different cultivation lots of the potato variety Van Gogh were used. Cultivation and harvesting conditions and peeling method were the most important facts reducing the sensory quality, especially the appearance, of prepeeled and sliced potatoes. Cooking and baking of potatoes decreased the appearance defects detected in raw potatoes. The levels of vitamin C in packaged samples decreased during winter storage. Cooking for 10 min and keeping potatoes at 60 degrees C for 1 h after cooking also decreased the content of vitamin C. In potato samples immediately after treatments aerobic bacteria were present at levels of 400 to 2,950 CFU/g and lactic acid bacteria at levels of 8 to 16 CFU/g. The number of aerobic bacteria did not increase during storage, and the number of lactic acid bacteria increased at the most to 90 CFU/g. Peeling, washing and packaging methods, cultivation conditions, and winter storage did not have important effects on the number of microbes present.

  14. Inactivation of Salmonella on Sprouting Seeds Using a Spontaneous Carvacrol Nanoemulsion Acidified with Organic Acids.

    Science.gov (United States)

    Landry, Kyle S; Komaiko, Jennifer; Wong, Dana E; Xu, Ting; McClements, David Julian; McLandsborough, Lynne

    2016-07-01

    Over the past decade, demand has increased for natural, minimally processed produce, including sprout-based products. Sanitization with 20,000 ppm of calcium hypochlorite is currently recommended for all sprouting seeds before germination to limit sprout-related foodborne outbreaks. A potentially promising disinfectant as an alternative to calcium hypochlorite is acidified spontaneous essential oil nanoemulsions. In this study, the efficacy of an acidified carvacrol nanoemulsion was tested against mung beans and broccoli seeds artificially contaminated with a Salmonella enterica Enteritidis cocktail (ATCC BAA-709, ATCC BAA-711, and ATCC BAA-1045). Treatments were performed by soaking inoculated seeds in acidified (50 mM acetic or levulinic acid) carvacrol nanoemulsions (4,000 or 8,000 ppm) for 30 or 60 min. After treatment, the number of surviving cells was determined via plate counts and/or the most probable number (MPN) approach. Treatment for 30 min successfully reduced Salmonella Enteritidis by 4 log CFU/g on mung beans (from an initial contamination level of 4.2 to 4.6 log CFU/g) and by 2 log CFU/g on broccoli seeds (from an initial contamination level of 2.4 to 2.6 log CFU/g) to below our detection limit (≤3 MPN/g). Treated seeds were sprouted and tested for the presence of pathogens and sprout yield. The final sprout product had no detectable pathogens, and total sprout yield was not influenced by any treatment.

  15. Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts*

    Science.gov (United States)

    Guo, Rong-fang; Yuan, Gao-feng; Wang, Qiao-mei

    2013-01-01

    To understand the regulation mechanism of NaCl on glucosinolate metabolism in broccoli sprouts, the germination rate, fresh weight, contents of glucosinolates and sulforaphane, as well as myrosinase activity of broccoli sprouts germinated under 0, 20, 40, 60, 80, and 100 mmol/L of NaCl were investigated in our experiment. The results showed that glucoerucin, glucobrassicin, and 4-hydroxy glucobrassicin in 7-d-old broccoli sprouts were significantly enhanced and the activity of myrosinase was inhibited by 100 mmol/L of NaCl. However, the total glucosinolate content in 7-d-old broccoli sprouts was markedly decreased although the fresh weight was significantly increased after treatment with NaCl at relatively low concentrations (20, 40, and 60 mmol/L). NaCl treatment at the concentration of 60 mmol/L for 5 d maintained higher biomass and comparatively higher content of glucosinolates in sprouts of broccoli with decreased myrosinase activity. A relatively high level of NaCl treatment (100 mmol/L) significantly increased the content of sulforaphane in 7-d-old broccoli sprouts compared with the control. These results indicate that broccoli sprouts grown under a suitable concentration of NaCl could be desirable for human nutrition. PMID:23365011

  16. Calorific values for young sprouts of nine hardwood species

    Energy Technology Data Exchange (ETDEWEB)

    Neenan, M.; Steinbeck, K.

    Calorific content, specific gravity and ash content of 10 to 15 year old sprouts of nine hardwood species were determined in mid-summer. Energy values for wood, bark, first order branches, twigs and leaves were determined with an adiabatic bomb calorimeter. The values found for coppice material averaged 4791 cal/g and were comparable to published values for older wood. Intraspecific differences among tissues were greater than the relatively small but significant differences among species. Differences in yield potential are therefore likely to outweigh variations in calorific content among species where total energy production per unit of land area is concerned. Ash content varied from 0.65% for wood to 5.88% for bark.

  17. Caloric values for young sprouts of nine hardwood species

    Energy Technology Data Exchange (ETDEWEB)

    Neenan, M. (An Foras Taluntais, Oak Park, Carlow, Ireland); Steinbeck, K.

    1979-09-01

    Caloric content, specific gravity, and ash content of 6- to 15-year-old sprouts of nine hardwood species were determined in midsummer. Energy values for wood, bark, first order branches, twigs, and leaves were determined with an adiabatic bomb calorimeter. The values found for coppice material averaged 4791 cal/g and were comparable to published values for older wood. Intraspecific differences among tissues were greater than the relatively small but significant differences among species. Differences in yield potential are therefore likely to outweigh variations in caloric content among species where total energy production per unit of land area is concerned. Ash content varied from 0.65 percent for wood to 5.88 percent for bark.

  18. Use of radiation for inducing mutants in potatoes through tissue culture technique

    International Nuclear Information System (INIS)

    Sharabash, M.T.

    1997-01-01

    Meristem-tips obtained from sprouts of potato tubers, cv. 'Diamant' (Solanum tuberosum) were cultured on MS-medium and multiplied into plantlets through micropropagation. After 2-3 weeks, the micropropagated plantlets had 5-6 nodes each. The plantlets were irradiated with 20 to 40 Gy gamma rays at 27.7 rad/sec. Irradiated plantlets were cut into single nodes and cultured on MS-medium supplemented with 2 g NaCl. Salt resistant plantlets were transferred to MS-liquid medium supplemented with 2g NaCl/l 5.2, and microtubers were collected after 6 weeks. Minitubers were produced under the same level of salinity. (author). 2 tabs

  19. Isothiocyanate metabolism, distribution, and interconversion in mice following consumption of thermally processed broccoli sprouts or purified sulforaphane.

    Science.gov (United States)

    Bricker, Gregory V; Riedl, Kenneth M; Ralston, Robin A; Tober, Kathleen L; Oberyszyn, Tatiana M; Schwartz, Steven J

    2014-10-01

    Broccoli sprouts are a rich source of glucosinolates, a group of phytochemicals that when hydrolyzed, are associated with cancer prevention. Our objectives were to investigate the metabolism, distribution, and interconversion of isothiocyanates (ITCs) in mice fed thermally processed broccoli sprout powders (BSPs) or the purified ITC sulforaphane. For 1 wk, mice were fed a control diet (n = 20) or one of four treatment diets (n = 10 each) containing nonheated BSP, 60°C mildly heated BSP, 5-min steamed BSP, or 3 mmol purified sulforaphane. Sulforaphane and erucin metabolite concentrations in skin, liver, kidney, bladder, lung, and plasma were quantified using HPLC-MS/MS. Thermal intensity of BSP processing had disparate effects on ITC metabolite concentrations upon consumption. Mild heating generally resulted in the greatest ITC metabolite concentrations in vivo, followed by the nonheated and steamed BSP diets. We observed interconversion between sulforaphane and erucin species or metabolites, and report that erucin is the favored form in liver, kidney, and bladder, even when only sulforaphane is consumed. ITC metabolites were distributed to all tissues analyzed, suggesting the potential for systemic benefits. We report for the first time tissue-dependent ratio of sulforaphane and erucin, though further investigation is warranted to assess biological activity of individual forms. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Peanut cross-reacting allergens in seeds and sprouts of a range of legumes

    DEFF Research Database (Denmark)

    Jensen, Louise Bjerreman; Pedersen, Mona H; Skov, Per Stahl

    2008-01-01

    protein extracts of seeds and sprouts (comprising cotelydons and hypocotyls/epicotyls) of peanut, soybean, green pea, blue lupine, mung bean, alfalfa, broad bean, and azuki bean were prepared. The reactivity of sera from 10 peanut-allergic patients to these extracts was analysed by indirect histamine......BACKGROUND: Recently, peanut-allergic patients have reported symptoms upon ingestion of bean sprouts produced from various legumes. OBJECTIVE: This study was designed to identify immunoreactivity to seeds and sprouts of legumes other than peanut in sera from peanut-allergic patients. METHODS: Crude...

  1. Transcriptomic changes during tuber dormancy release process revealed by RNA sequencing in potato.

    Science.gov (United States)

    Liu, Bailin; Zhang, Ning; Wen, Yikai; Jin, Xin; Yang, Jiangwei; Si, Huaijun; Wang, Di

    2015-03-20

    Potato tuber dormancy release is a critical development process that allows potato to produce new plant. The first Illumina RNA sequencing to generate the expressed mRNAs at dormancy tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) was performed. We identified 26,639 genes including 5,912 (3,450 up-regulated while 2,462 down-regulated) and 3,885 (2,141 up-regulated while 1,744 down-regulated) genes were differentially expressed from DT vs DRT and DRT vs ST. The RNA-Seq results were further verified using qRT-PCR. We found reserve mobilization events were activated before the bud emergence (DT vs DRT) and highlighted after dormancy release (DRT vs ST). Overexpressed genes related to metabolism of auxin, gibberellic acid, cytokinin and barssinosteriod were dominated in DT vs DRT, whereas overexpressed genes involved in metabolism of ethylene, jasmonate and salicylate were prominent in DRT vs ST. Various histone and cyclin isoforms associated genes involved in cell division/cycle were mainly up-regulated in DT vs DRT. Dormancy release process was also companied by stress response and redox regulation, those genes related to biotic stress, cell wall and second metabolism was preferentially overexpressed in DRT vs ST, which might accelerate dormancy breaking and sprout outgrowth. The metabolic processes activated during tuber dormancy release were also supported by plant seed models. These results represented the first comprehensive picture of a large number of genes involved in tuber dormancy release process. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Prevention

    Science.gov (United States)

    ... Contact Aging & Health A to Z Find a Geriatrics Healthcare Professional Medications & Older Adults Making Your Wishes ... Prevention Hearing Loss Heart Attack High Blood Pressure Nutrition Osteoporosis Shingles Skin Cancer Related News Quitting Smoking, ...

  3. Assessing the processing quality of different potato cultivars during storage at various temperatures

    International Nuclear Information System (INIS)

    Amjad, A.; Randhawa, M.A.; Butt, M.S.; Asghar, M.

    2016-01-01

    Processing industry needs continuous supply of tubers for fries/chips preparation throughout the year. Storage is obligatory to meet the increasing demand of population. Objective of this study was to evaluate the processing and quality characteristics of different potato cultivars (Lady Rosetta, Sante, Hermes, Crozo, Kuroda and Asterix) during storage with 75-80 percent relative humidity for the period of 160 days at various temperatures (3 degree C, 7 degree C, 11 degree C). Quality parameters such as specific gravity, sprouting, weight loss, dry matter, starch content, ascorbic acid, sugar content and invertase enzyme activity were determined to estimate the processing potential of each cultivar. High Performance Liquid Chromatography (HPLC) equipped with amino (NH2) column and Refractive Index Detector (RID) was used for the identification and quantification of sugars. The findings of the present work showed that temperature significantly (p < 0.05) influenced the processing quality throughout the storage. Low temperature (3 degree C) storage caused cold induced sweetening (CIS) due to increased invertase activity whereas, high temperature resulted in sprouting and weight loss of the tubers. Overall processing acceptability for all the cultivars with good frying color was obtained at an intermediate storage temperature of 7 degree C in the order of Lady Rosetta > Hermes > Crozo > Sante > Asterix > Kuroda. (author)

  4. Stem girdling evidences a trade-off between cambial activity and sprouting and dramatically reduces plant transpiration due to feedback inhibition of photosynthesis and hormone signaling

    Directory of Open Access Journals (Sweden)

    Rosana eLópez

    2015-04-01

    Full Text Available The photosynthesis source-sink relationship in young Pinus canariensis seedlings was modified by stem girdling to investigate sprouting and cambial activity, feedback inhibition of photosynthesis, and stem and root hydraulic capacity. Removal of bark tissue showed a trade-off between sprouting and diameter growth. Above the girdle, growth was accelerated but the number of sprouts was almost negligible, whereas below the girdle the response was reversed. Girdling resulted in a sharp decrease in whole plant transpiration and root hydraulic conductance. The reduction of leaf area after girdling was strengthened by the high levels of ABA found in buds which pointed to stronger bud dormancy, preventing a new needle flush. Accumulation of sugars in leaves led to a coordinated reduction in net photosynthesis (AN and stomatal conductance (gS in the short term, but later (gS below 0.07 mol m-2 s-1 AN decreased faster. The decrease in maximal efficiency of photosystem II (FV/FM and the operating quantum efficiency of photosystem II (ΦPSII in girdled plants could suggest photoprotection of leaves, as shown by the vigorous recovery of AN and ΦPSII after reconnection of the phloem. Stem girdling did not affect xylem embolism but increased stem hydraulic conductance above the girdle. This study shows that stem girdling affects not only the carbon balance, but also the water status of the plant.

  5. Comparison of Anti-Inflammatory Effects of Flavonoid-Rich Common and Tartary Buckwheat Sprout Extracts in Lipopolysaccharide-Stimulated RAW 264.7 and Peritoneal Macrophages

    Directory of Open Access Journals (Sweden)

    Tae Gyu Nam

    2017-01-01

    Full Text Available Buckwheat sprouts have been widely consumed all around world due to their great abundance of bioactive compounds. In this study, the anti-inflammatory effects of flavonoid-rich common buckwheat sprout (CBS and tartary buckwheat sprout (TBS extracts were evaluated in lipopolysaccharide- (LPS- stimulated RAW 264.7 murine macrophages and primary peritoneal macrophages from male BALB/c mice. Based on the reversed-phase HPLC analysis, the major flavonoids in CBS were determined to be C-glycosylflavones (orientin, isoorientin, vitexin, and isovitexin, quercetin-3-O-robinobioside, and rutin, whereas TBS contained only high amounts of rutin. The TBS extract exhibited higher inhibitory activity as assessed by the production of proinflammatory mediators such as nitric oxide and cytokines including tumor necrosis factor-α, interleukin- (IL- 6, and IL-12 in LPS-stimulated RAW 264.7 macrophages than CBS extract. In addition, TBS extract suppressed nuclear factor-kappa B activation by preventing inhibitor kappa B-alpha degradation and mitogen-activated protein kinase phosphorylation in LPS-stimulated RAW 264.7 macrophages. Moreover, the TBS extract markedly reduced LPS-induced cytokine production in peritoneal macrophages. Taken together, these findings suggest that TBS extract can be a potential source of anti-inflammatory agents that may influence macrophage-mediated inflammatory disorders.

  6. The effects of monitoring the abundance and species composition of aphids as virus vectors on seed potato production in Serbia

    Directory of Open Access Journals (Sweden)

    Drago Milošević

    2014-03-01

    Full Text Available Aphids are the most important vectors of potato viruses during the crop’s growing season. The most widespread and damaging viruses, the potato virus Y and potato leaf roll virus, are transmitted by aphids in non-persistent and persistent manner, respectively. The two viruses cause the greatest concern of potato producers and a great constraint to seed potato production in Serbia, the region and across the world. Potato virus Y is particularly harmful, given its distribution and spreading rate. Seed potato production systems under well-managed conditions involve a series of virus control measures, including the monitoring of outbreaks of winged aphids, their abundance and species composition, in order to forecast virosis, i.e. potential plant and tuber infection periods. Monitoring the aphid vectors of potato viruses enables determination of optimum dates for haulm destruction when higher than normal numbers of winged aphids as vectors of economically harmful diseases have been observed. Haulm destruction in a potato crop reduces the risk of plant infection and virus translocation from the aboveground parts to tubers, thus keeping the proportion of infected tubers within tolerance limits allowed for certain categories of seed potatoes. This practice has positive effects if used in combination with other viral disease control measures; otherwise, it becomes ineffective. This paper provides an integral analysis of the effects and role of monitoring outbreaks of aphids, their abundance and species composition in timing haulm growth termination to prevent plant infection, virus translocation and tuber infestation in potato crops in Serbia and the wider region.

  7. Acrylamide in Fried Potato Products

    NARCIS (Netherlands)

    Luning, Pieternel; Sanny, Maimunah

    2016-01-01

    High acrylamide levels have been detected in fried potato products. Dietary intake studies observed large differences in acrylamide between single foodstuffs, within food categories, and within batches of similarly processed products. FAO/WHO emphasized that causes of variation need to be

  8. Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization

    DEFF Research Database (Denmark)

    Wild, Raphael; Klems, Alina; Takamiya, Masanari

    2017-01-01

    Formation of organ-specific vasculatures requires cross-talk between developing tissue and specialized endothelial cells. Here we show how developing zebrafish spinal cord neurons coordinate vessel growth through balancing of neuron-derived Vegfaa, with neuronal sFlt1 restricting Vegfaa......-Kdrl mediated angiogenesis at the neurovascular interface. Neuron-specific loss of flt1 or increased neuronal vegfaa expression promotes angiogenesis and peri-neural tube vascular network formation. Combining loss of neuronal flt1 with gain of vegfaa promotes sprout invasion into the neural tube. On loss...... of neuronal flt1, ectopic sprouts emanate from veins involving special angiogenic cell behaviours including nuclear positioning and a molecular signature distinct from primary arterial or secondary venous sprouting. Manipulation of arteriovenous identity or Notch signalling established that ectopic sprouting...

  9. Breeding increases the efficacy of Chondrostereum purpureum in the sprout control of birch.

    Directory of Open Access Journals (Sweden)

    Leena Hamberg

    Full Text Available We tested whether the pairing of selected isolates could be used to increase the efficiency of a decay fungus Chondrostereum purpureum (Pers. Ex Fr. Pouzar to control hardwood sprouting in Finland. We paired C. purpureum strains efficient in sprout control or highly active in laccase production, and tested the efficacy of their progeny in spout control experiments. This procedure resulted in a strain with an efficacy superior to that of the parental strains. The mortality of birch (Betula pendula Roth. and B. pubescens Ehrh. 1 cm in stump diameter was 78%, 56% and 9% for the best progeny, the best parental strain and the control, respectively. Mortality was only slightly higher for B. pendula than for B. pubescens but no significant differences were found between the number or maximum height of stump sprouts. Our results showed that cross breeding of this decay fungus is a good alternative in attempts to produce efficient biocontrol agents against hardwood sprouting.

  10. The effect of the germination temperature on the phytochemical content of broccoli and rocket sprouts.

    Science.gov (United States)

    Ragusa, Lucia; Picchi, Valentina; Tribulato, Alessandro; Cavallaro, Chiara; Lo Scalzo, Roberto; Branca, Ferdinando

    2017-06-01

    This study investigates the effect of different germination temperatures (10, 20 and 30 °C) on the phytochemical content as well as reducing and antioxidant capacity of broccoli and rocket sprouts. In both seeds and sprouts, the total glucosinolates and ascorbic acid contents did not differ between vegetables, while broccoli exhibited exceptionally higher polyphenols and greater reducing and antioxidant capacity compared to rocket. In both species, an increase in germination temperature positively affected the glucosinolate content. Ascorbic acid increased during germination without a difference among the three tested temperatures. The phenol content in broccoli sprouts increased when they were grown at 30 °C, but the amount decreased at the highest temperatures in rocket. The reducing and antioxidant capacities increased with germination, and higher indexes were detected at 10 °C, particularly in rocket. Different germination temperatures differentiate the health-promoting phytochemical content and antioxidant properties in broccoli and rocket sprouts.

  11. Is stump sprout treatment necessary to effectively control Phytophthora ramorum in California's wildlands?

    Science.gov (United States)

    Yana Valachovic; Richard Cobb; David Rizzo; Brendan Twieg; Chris Lee; Radoslaw Glebocki

    2013-01-01

    In California, wildland hosts that support sporulation of Phytophthora ramorum, such as California bay laurel (Umbellularia californica (Hook. & Arn.) Nutt.) and tanoak (Notholithocarpus densiflorus (Hook. & Arn.) Manos, Cannon & S.H. Oh), also develop prolific basal sprouts following...

  12. Prevention

    DEFF Research Database (Denmark)

    Halken, S; Høst, A

    2001-01-01

    , breastfeeding should be encouraged for 4-6 months. In high-risk infants a documented extensively hydrolysed formula is recommended if exclusive breastfeeding is not possible for the first 4 months of life. There is no evidence for preventive dietary intervention neither during pregnancy nor lactation...... populations. These theories remain to be documented in proper, controlled and prospective studies. Breastfeeding and the late introduction of solid foods (>4 months) is associated with a reduced risk of food allergy, atopic dermatitis, and recurrent wheezing and asthma in early childhood. In all infants....... Preventive dietary restrictions after the age of 4-6 months are not scientifically documented....

  13. Postharvest changes in glycoalkaloid content of potatoes.

    Science.gov (United States)

    Friedman, M; McDonald, G M

    1999-01-01

    Potatoes contain antinutritional and potentially toxic compounds including inhibitors of digestive enzymes, hemagglutinins, and glycoalkaloids. Solanum glycoalkaloids are reported to inhibit cholinesterase, disrupt cell membranes, and induce teratogenicity. In this overview, we describe the role of potatoes in the human diet, reported changes in glycoalkaloid content of fresh and processed potatoes during storage, under the influence of light and radiation, following mechanical damage, and as a result of food processing. Also covered are safety aspects and suggested research needs to develop a protocol that can be adopted by the potato producers and processors to minimize post-harvest synthesis of glycoalkaloids in potatoes. Reducing the glycoalkaloid content of potatoes will provide a variety of benefits extending from the farm to processing, shipping, marketing, and consumption of potatoes and potato products. A commercially available ELISA kit is described which permits rapid assay of glycoalkaloid content of parts of the potato plant including leaves, tubers, and peel, as well as processed potato products including french fries, chips, and skins. Understanding the multiple overlapping aspects of glycoalkaloids in the plant and in the diet will permit controlling postharvest glycoalkaloid production for the benefit of the producer and consumer.

  14. The occurrence of Salmonella in raw and ready-to-eat bean sprouts and sprouted seeds on retail sale in England and Northern Ireland.

    Science.gov (United States)

    Sadler-Reeves, L; Aird, H; de Pinna, E; Elviss, N; Fox, A; Kaye, M; Jorgensen, F; Lane, C; Willis, C; McLauchlin, J

    2016-02-01

    A total of 554 samples of bean sprouts or other sprouted seeds were collected at retail sale and submitted to nine Official Control Laboratories in England and Northern Ireland during January to March 2011. Samples (100 g) were tested for the presence of Salmonella using the EN ISO 6579:2002 method. Products labelled as ready-to-eat comprised 23% of the samples and 61% were labelled as raw or to-cook: the remaining 12% had no indication if the food was intended as ready-to-eat or ready-to-cook, and 4% were not recorded. Salmonella spp. were detected from four samples of mung-bean sprouts (0·7% of all the 554 samples) and all four isolates were confirmed as Salmonella enterica serovar Abaetetuba (11 : k : 1,5). Two of the samples where Salmonella was detected were sold as ready-to-eat (labelled 'rinse and serve' only): The remaining two were from samples labelled as ready-to-cook. Consumption of sprouted seeds have been associated with infections from a range of foodborne pathogens, particularly Salmonella and shigatoxin producing Escherichia coli (STEC). However, there is limited data (including that from EU monitoring) on foodborne pathogens in samples of this food type which are not associated with outbreaks of infection. Out of 554 raw and ready-to-eat bean sprouts and sprouted seeds sampled at retail, Salmonella spp. was detected from four samples. This study illustrated the potential of this product to be contaminated with a human pathogen and the importance of considering the intended use and preparation of specific food in assessing microbiological risks. © 2015 The Society for Applied Microbiology.

  15. Evaluation of chlorine dioxide gas treatment to inactivate Salmonella enterica on mungbean sprouts.

    Science.gov (United States)

    Prodduk, Vara; Annous, Bassam A; Liu, Linshu; Yam, Kit L

    2014-11-01

    Although freshly sprouted beans and grains are considered to be a source of nutrients, they have been associated with foodborne outbreaks. Sprouts provide good matrices for microbial localization and growth due to optimal conditions of temperature and humidity while sprouting. Also, the lack of a kill step postsprouting is a major safety concern. The objective of this work was to evaluate the effectiveness of chlorine dioxide gas treatment to reduce Salmonella on artificially inoculated mungbean sprouts. The effectiveness of gaseous chlorine dioxide (0.5 mg/liter of air) with or without tumbling (mechanical mixing) was compared with an aqueous chlorine (200 ppm) wash treatment. Tumbling the inoculated sprouts during the chlorine dioxide gas application for 15, 30, and 60 min reduced Salmonella populations by 3.0, 4.0, and 5.5 log CFU/g, respectively, as compared with 3.0, 3.0, and 4.0 log CFU/g reductions obtained without tumbling, respectively. A 2.0 log CFU/g reduction in Salmonella was achieved with an aqueous chlorine wash. The difference in microbial reduction between chlorine dioxide gas versus aqueous chlorine wash points to the important role of surface topography, pore structure, bacterial attachment, and/or biofilm formation on sprouts. These data suggested that chlorine dioxide gas was capable of penetrating and inactivating cells that are attached to inaccessible sites and/or are within biofilms on the sprout surface as compared with an aqueous chlorine wash. Consequently, scanning electron microscopy imaging indicated that chlorine dioxide gas treatment was capable of penetrating and inactivating cells attached to inaccessible sites and within biofilms on the sprout surfaces.

  16. Enrichment of Probiotic Yogurt with Broccoli Sprout Extract and its Effect on Helicobacter pylori

    Directory of Open Access Journals (Sweden)

    Ali Reza Sadeghi

    2017-01-01

    Full Text Available Background and Objective: Antibiotic consumption is the main way to cure infection induced by Helicobacter pylori. On the other hand, antibiotics have side effects on human body. So, finding an efficient way to replace antibiotic consumption seems necessary. The aim of this study was to investigate the effect of broccoli sprout extract on the viability of probiotic bacteria and yogurt’s physicochemical properties, and examine the synergistic effect of this extract with probiotics on Helicobacter pylori growth inhibition.Material and Methods: Four levels of broccoli sprout extract (22.5, 45, 90 and 180 mg ml-1 were prepared and their effect on probiotic yogurt samples was examined. Moreover, their anti- Helicobacter pylori effect was determined.Results and Conclusion: The research results revealed that Broccoli sprout extract did not have any inhibitory effect on Bifidobacterium lactis and Lactobacillus acidophilus. The variations in acidity of the samples were not significant during storage. A positive correlation was observed between broccoli sprout extract concentration and syneresis. The findings showed the synergistic effect of broccoli sprout extract and probiotics on Helicobacter pylori growth inhibition. Therefore, using broccoli sprout extract and probiotic bacteria, we can produce a yogurt that is effective on the growth inhibition of Helicobacter pylori.Conflict of interest: The authors declare that there is no conflict of interest.

  17. Effect of corona discharge plasma jet on surface-borne microorganisms and sprouting of broccoli seeds.

    Science.gov (United States)

    Kim, Je-Wook; Puligundla, Pradeep; Mok, Chulkyoon

    2017-01-01

    Different pathogenic microorganisms have been reported to cause sprouts-associated outbreaks. In order to sterilise and enhance the germination of seeds, non-thermal plasma has been increasingly investigated in the field of agricultural science as an alternative to the traditional pre-sowing seed treatments. This work aimed to evaluate the effect of corona discharge plasma jet (CDPJ) on disinfection of the natural bio-contaminants of broccoli seed and also studied the plasma effect on sprout seed germination rate and physico-chemical properties of sprouts. Aerobic bacteria, moulds and yeasts, B. cereus, E. coli, Salmonella spp. were detected on the broccoli seed surface. After 0-3 min treatment using CDPJ, the detected microorganisms were reduced in the range of 1.2-2.3 log units. Inactivation patterns were better explained using pseudo-first-order kinetics. The plasma treatment of seeds up to 2 min exhibited a positive effect on germination rate, seedling growth. The physico-chemical and sensory characteristics of sprouts were unaffected due to the CDPJ treatment of their respective seeds. Corona discharge plasma jet can potentially be used for microbial decontamination of broccoli seeds. In addition, the plasma treatment of broccoli sprout seeds has enabled a significant enhancement in their germination rate and seedling growth without compromising physico-chemical and sensory characteristics of their corresponding sprouts. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  18. Variation of unsaturated fatty acids in soybean sprout of high oleic acid accessions.

    Science.gov (United States)

    Dhakal, Krishna Hari; Jung, Ki-Hwal; Chae, Jong-Hyun; Shannon, J Grover; Lee, Jeong-Dong

    2014-12-01

    Oleic acid and oleic acid rich foods may have beneficial health effects in humans. Soybeans with high oleic acid (around 80% in seed oil) have been developed. Soybean sprouts are an important vegetable in Korea, Japan and China. The objective of this study was to investigate the variation of unsaturated fatty acids, oleic, linoleic and α-linolenic acids, in sprouts from soybeans with normal and high oleic acid concentration. Twelve soybean accessions with six high oleic acid lines, three parents of high oleic acid lines, and three checks with normal and high oleic acid concentration were used in this study. The unsaturated fatty acid concentration in sprouts from each genotype was similar to the concentration in the ungerminated seed. The oleic acid concentration in the sprouts of high oleic acid lines (up to 80%) was still high (>70%) compared to the ungerminated seed. Thus, high oleic soybean varieties developed for sprout production could add valuable health benefits to sprouts and the individuals who consume this vegetable. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Homocysteine Facilitates Prominent Polygonal Angiogenetic Networks of a Choroidal Capillary Sprouting Model.

    Science.gov (United States)

    Lee, Yih-Jing; Chiu, Chien-Chao; Ke, Chia-Ying; Tien, Ni; Lin, Po-Kang

    2017-08-01

    To investigate the effects of homocysteine on choroidal angiogenesis, we established an ex vivo choroidal sprouting explant model and examined the potential growth factors for angiogenesis. Choroid fragments with retinal pigment epithelium were isolated from mouse and embedded in Matrigel. Homocysteine at different concentrations were added to the culture mediums. The choroidal explants were observed at different time points, and the total area of choroidal sprouting was measured and analyzed. Homocysteine evoked choroidal capillary sprouting by inducing capillary endothelial cell proliferation with pericyte formation and by facilitating polygonal angiogenetic networks. In some cases, vascular lumens were observed in the newly forming capillaries facilitated by homocysteine. The choroidal sprouting effect of homocysteine can only be observed at a certain range of homocysteine concentration, with 1-mM homocysteine exhibiting the most significantly increased choroidal sprouting areas. Isolectin overexpression was noted in the homocysteine-treated group. Possible growth factors for angiogenesis were detected through immunofluorescent staining, which demonstrated the overexpression of platelet-derived growth factor C and angiopoietin 1 in the homocysteine-treated preparations only. In these preparations, platelet-derived growth factor C was highly expressed in the tip cells of sprouting capillaries. We therefore conclude that platelet-derived growth factor C and angiopoietin 1 may play key roles in the choroid angiogenesis evoked by homocysteine.

  20. Study the relationship between the multi embryo and the sprout inhibition of onion by gamma irradiation

    International Nuclear Information System (INIS)

    Al-Bachir, M.

    2006-04-01

    To determine the possible effect on sprout inhibition of onions, Indolbutyric acid (IBA) and cytokinins (5000 ppm), low doses of gamma irradiation (0, 30, 60, 90 and 120 Gy) in a 60 Co package irradiator, combined treatment of IBA and cytokinin followed by irradiation, were applied on the cultivars Baladi red and Baladi white produced in middle and south area of Syria. Treatments were done immediately and after 4, 7, 10 weeks of harvest. After treated and untreated samples were stored at room temperature. Weight loss, sprouting percentage and length of sprouting were determined after 8 months of storage. Results indicated that, there were no significant differences (P<0.05) in weight loss, sprouting percentage and length of sprouting between the treated and untreated bulbs with IBA and cytokinin. Gamma irradiation reduced the weight loss, and inhibited the sprouting when the irradiation were done after 4 weeks of harvest, and reduced it when the irradiation were done after 7 or 10 weeks of harvest. (author)

  1. Population dynamics of potato cyst nematodes and associated damage to potato

    NARCIS (Netherlands)

    Schans, J.

    1993-01-01

    Population dynamics of potato cyst nematodes (PCN; Globoderarostochiensis (Woll.) Skarbilovich and G. pallida Stone) and their interactions with potato plants are insufficiently understood to explain variations of population

  2. Granule size affects substitution on amylopectin populations in potato and sweet potato starches

    NARCIS (Netherlands)

    Chen, Z.; Huang, J.; Suurs, P.; Schols, H.A.; Voragen, A.G.J.

    2005-01-01

    Specific enzymatic degradation in combination with chromatographic and spectrometric techniques was used to understand acetyl group distribution over the amylopectin populations of differently sized granule fractions from potato and sweet potato starches. The hydrolysates obtained after ¿-amylase,

  3. Physico-chemical and functional properties of potato proteins

    NARCIS (Netherlands)

    Koningsveld, van G.A.

    2001-01-01

    Key words: potato proteins, patatin, protease inhibitors, solubility, structure, pH, temperature, ethanol, ionic strength, phenolic compounds, foams, emulsions

    In potato starch manufacture an aqueous byproduct remains that is called potato

  4. Radiation-induced sprout and growth inhibition in vegetables with special reference to the susceptibility to microbial attacks and the effect of calcium

    International Nuclear Information System (INIS)

    Skou, J.P.

    1979-03-01

    Experiments have shown ionizing irradiation to be an effective method for sprout and growth inhibition but it is necessary to keep the doses at the absolute minimum in order to avoid unwanted by-effects One of the by-effects is an increased susceptibility to storage rot in potatoes, onions and carrots. This effect is connected with the wounding and bruising caused by digging up and handling as the wound healing process is inhibited simultaneously with the sprout inhibition. Patogens increase tissue permeability during pathogenesis and, as irradiation has an analogous effect on tissues it might facilitate the growth of the pathogens. Irradiation softens the tissue and mobilizes the calcium in the tissue; this may thereby make the tissue more accessible to microbial attack. An external supply of calcium increases the firmness of tissue, reduces tissue permeability, and may compensate for the loss of calcium in irradiated tissue mainly as a result of a surplus of calcium in the wounds. Botrytis cinerea and Sclerotinia sclerotiorum were some of the most wide spread and serious pathogens in carrots, which vegetable were the main object of the studies. Culture filtrates of these fungi had a strong macerating activity on carrot tissues. The effect, which results from activity and interaction of pectolytic enzymes and oxalic acid, could be reduced or nullified by calcium. A diversity of the groups of pectolytic enzymes are widely distributed among organisms and not confined to plant pathogens. Because of this, because there exists pectolytic enzymes for every condition and pectic substances, and because calcium is not very inhibiting to all kinds of pectolytic enzymes it is not to be expected that the protective effect of calcium will always be expressed to the same extent on storage of the products. (author)

  5. "Couch-potatoeism" and childhood obesity: The inverse causality hypothesis.

    Science.gov (United States)

    Fröberg, Andreas

    2015-04-01

    The bulk of cross-sectional studies suggests that lower levels of physical activity are associated with childhood obesity. Although this has led to the general understanding that "couch-potatoes" are fat on account of their inactive lifestyles, cross-sectional studies do not imply causality. On the contrary, the contribution of physical activity to obesity during childhood is currently unclear, and lately, studies have suggested that "couch-potatoeism" could be the result of obesity rather than its cause. Coupled with evidence suggesting that interventions have had little effect on children's physical activity levels as well as on obesity, this inverse causality challenges the role of physical activity in childhood obesity prevention strategies. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. Complete Genome Sequences of Sweet potato feathery mottle virus and Sweet potato virus G from Brazil

    OpenAIRE

    Souza, Caroline A.; Rossato, Maurício; Melo, Fernando L.; Pereira-Carvalho, Rita C.

    2018-01-01

    ABSTRACT In Brazil, Potyvirus species in sweet potatoes have been detected mostly by serology. Here, we report the complete genome sequences of two Potyvirus species, Sweet potato feathery mottle virus strain (SPFMV-UNB-01) and Sweet potato virus G strain (SPVG-UNB-01).

  7. The role of entanglement concentration on the hydrodynamic properties of potato and sweet potato starches.

    Science.gov (United States)

    Guo, Li; Hu, Jian; Zhang, Juanjuan; Du, Xianfeng

    2016-12-01

    The hydrodynamic properties of potato starch and sweet potato starch in dilute and semi-dilute aqueous solutions were studied using a Ubbelohde viscometer, a transmission electron microscope, and steady shear rheological measurements. The results indicated that the potato starch solutions showed a linear shape of the η red versus c curves. The sweet potato starch solutions presented a non-linear shape with a downturn in dilute solutions, or the concentrations were lower than entanglement concentration (c e ). The c e values of the potato and sweet potato starch solutions were 0.43% and 0.54%, respectively. These findings indicated that the impact of the c e value on the network formation of the potato starch solutions was much more significant compared with the impact on the sweet potato starch solutions. The potato and sweet potato starch solutions showed shear thinning behaviour hardly occurs when the concentrations were less than c e , while shear thinning behaviour approached when the concentrations were equal to or greater than c e . Similarly, the potato and sweet potato starch solutions rarely resembled a pseudoplastic state when the concentrations were lower than or equal to c e , while the pseudoplastic behaviour developed when the concentrations were higher than c e . Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Anticancer and Antioxidant Activity of Bread Enriched with Broccoli Sprouts

    Science.gov (United States)

    Gawlik-Dziki, Urszula; Świeca, Michał; Dziki, Dariusz; Sęczyk, Łukasz; Złotek, Urszula; Różyło, Renata; Kaszuba, Kinga; Ryszawy, Damian; Czyż, Jarosław

    2014-01-01

    This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS) in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities); however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS) was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention. PMID:25050366

  9. Anticancer and antioxidant activity of bread enriched with broccoli sprouts.

    Science.gov (United States)

    Gawlik-Dziki, Urszula; Świeca, Michał; Dziki, Dariusz; Sęczyk, Łukasz; Złotek, Urszula; Różyło, Renata; Kaszuba, Kinga; Ryszawy, Damian; Czyż, Jarosław

    2014-01-01

    This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS) in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities); however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS) was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention.

  10. Anticancer and Antioxidant Activity of Bread Enriched with Broccoli Sprouts

    Directory of Open Access Journals (Sweden)

    Urszula Gawlik-Dziki

    2014-01-01

    Full Text Available This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities; however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention.

  11. Antioxidant capacity of broccoli sprouts subjected to gastrointestinal digestion.

    Science.gov (United States)

    Rychlik, Joanna; Olejnik, Anna; Olkowicz, Mariola; Kowalska, Katarzyna; Juzwa, Wojciech; Myszka, Kamila; Dembczyński, Radosław; Moyer, Mary Pat; Grajek, Włodzimierz

    2015-07-01

    Broccoli is a common vegetable recognized as a rich source of antioxidants. To date, research on the antioxidant properties of broccoli, predominantly conducted on extracts, has not considered the lesions of composition and this activity after gastrointestinal digestion. Here the stability of antioxidants during gastrointestinal digestion was evaluated in conjunction with the protective effects of broccoli sprouts (BS) against oxidative stress in human colon cells. The obtained data suggest that, among the biocompounds identified in BS, glucosinolates were mainly degraded under gastrointestinal digestion, while phenolics, particularly hydroxycinnamic acid derivatives, were the most resistant constituents. The antioxidant capacity of BS extract subjected to gastrointestinal digestion was similar to or higher than that determined for non-digested BS. Gastrointestinal digested BS extract exhibited reactive oxygen species (ROS)-inhibitory capacity in NCM460 human colon cells, with 1 mg mL(-1) showing an ROS clearance of 76.59%. A 57.33% reduction in oxidative DNA damage in NCM460 cells due to treatment with digested BS extract was observed. The results lend support to the possible application of BS as a rich source of antioxidants to improve the defensive system against oxidative stress in the human colon mucosa. © 2014 Society of Chemical Industry.

  12. QTL mapping for microtuber dormancy and GA3content in a diploid potato population.

    Science.gov (United States)

    Naz, Raja Mohib Muazzam; Li, Mengtai; Ramzan, Safia; Li, Gege; Liu, Jun; Cai, Xingkui; Xie, Conghua

    2018-01-23

    The genetic control of dormancy is poorly understood in most plant species, but dormancy is a prominent feature for the potato industry. We used the microtuber system, in which tubers were produced in vitro and stored at 20°C, to perform quantitative trait locus (QTL) analysis for dormancy and gibberellic acid (GA 3 ) content in an F 1 population consisting of 178 genotypes derived from an interspecific cross between Solanum chacoense acc. PI 320285 (long dormancy) and Solanum phureja acc. DM1-3 516 R44 (short dormancy). In this analysis, 163 markers were used to construct a genetic map with a total length of 591.8 cM. Through QTL analysis, we identified 22 markers closely linked to the timing of dormancy release and GA 3 content. The male parent alleles were closely related with long dormancy, with the most significant effect on chromosome I, which accounted for 9.4% of phenotypic variation. The dormancy and GA 3 QTLs localized to the same position in the genome, confirming that same genomic region controls GA 3 content at different developmental stages or in dormant and sprouting tubers. The identified QTLs may be useful for future breeding strategies and studies of dormancy in potato. © 2018. Published by The Company of Biologists Ltd.

  13. Work of multiple organizations to improve seed potato health in U.S.A. and an example of change to reduce Potato virus Y in seed potato lots

    Science.gov (United States)

    Work of multiple organizations to improve seed potato health in U.S.A. and an example of change to reduce Potato virus Y in seed lots. In the United States, seed potato improvement starts with the individual seed potato grower. The seed grower also has resources that are available from university e...

  14. Bioproducts from Potatoes. A Review

    Science.gov (United States)

    Priedniece, Vivita; Spalvins, Kriss; Ivanovs, Kaspars; Pubule, Jelena; Blumberga, Dagnija

    2017-12-01

    The increasing amount of food waste througout the world is becoming a major problem for waste management plants. The food waste produced amounts to 1.3 million tons a year. This is a resource that could be used for production of new products. Decreasing fossil resources and a rapidly growing population lead to the necessity to produce more food and to replace existing with new materials ones that are biological and produce little effect on environment. Bioeconomy is a method that can help achieve production of value-added products that use local resources and waste to manufacture products efficiently. In this article, we are looking at possibilities to use potatoes for production of new materials, such as bioplastics, antioxidants, proteins, instead of their conventional use for food production. We have studied potato components, extraction technologies and summed up possible directions for development for new products, looking at the use of processing waste as a raw material.

  15. German outbreak of Escherichia coli O104:H4 associated with sprouts.

    Science.gov (United States)

    Buchholz, Udo; Bernard, Helen; Werber, Dirk; Böhmer, Merle M; Remschmidt, Cornelius; Wilking, Hendrik; Deleré, Yvonne; an der Heiden, Matthias; Adlhoch, Cornelia; Dreesman, Johannes; Ehlers, Joachim; Ethelberg, Steen; Faber, Mirko; Frank, Christina; Fricke, Gerd; Greiner, Matthias; Höhle, Michael; Ivarsson, Sofie; Jark, Uwe; Kirchner, Markus; Koch, Judith; Krause, Gérard; Luber, Petra; Rosner, Bettina; Stark, Klaus; Kühne, Michael

    2011-11-10

    A large outbreak of the hemolytic-uremic syndrome caused by Shiga-toxin-producing Escherichia coli O104:H4 occurred in Germany in May 2011. The source of infection was undetermined. We conducted a matched case-control study and a recipe-based restaurant cohort study, along with environmental, trace-back, and trace-forward investigations, to determine the source of infection. The case-control study included 26 case subjects with the hemolytic-uremic syndrome and 81 control subjects. The outbreak of illness was associated with sprout consumption in univariable analysis (matched odds ratio, 5.8; 95% confidence interval [CI], 1.2 to 29) and with sprout and cucumber consumption in multivariable analysis. Among case subjects, 25% reported having eaten sprouts, and 88% reported having eaten cucumbers. The recipe-based study among 10 groups of visitors to restaurant K included 152 persons, among whom bloody diarrhea or diarrhea confirmed to be associated with Shiga-toxin-producing E. coli developed in 31 (20%). Visitors who were served sprouts were significantly more likely to become ill (relative risk, 14.2; 95% CI, 2.6 to ∞). Sprout consumption explained 100% of cases. Trace-back investigation of sprouts from the distributor that supplied restaurant K led to producer A. All 41 case clusters with known trading connections could be explained by producer A. The outbreak strain could not be identified on seeds from the implicated lot. Our investigations identified sprouts as the most likely outbreak vehicle, underlining the need to take into account food items that may be overlooked during subjects' recall of consumption.

  16. Gene expression profiles predictive of cold-induced sweetening in potato.

    Science.gov (United States)

    Neilson, Jonathan; Lagüe, M; Thomson, S; Aurousseau, F; Murphy, A M; Bizimungu, B; Deveaux, V; Bègue, Y; Jacobs, J M E; Tai, H H

    2017-07-01

    Cold storage (2-4 °C) used in potato production to suppress diseases and sprouting during storage can result in cold-induced sweetening (CIS), where reducing sugars accumulate in tuber tissue leading to undesirable browning, production of bitter flavors, and increased levels of acrylamide with frying. Potato exhibits genetic and environmental variation in resistance to CIS. The current study profiles gene expression in post-harvest tubers before cold storage using transcriptome sequencing and identifies genes whose expression is predictive for CIS. A distance matrix for potato clones based on glucose levels after cold storage was constructed and compared to distance matrices constructed using RNA-seq gene expression data. Congruence between glucose and gene expression distance matrices was tested for each gene. Correlation between glucose and gene expression was also tested. Seventy-three genes were found that had significant p values in the congruence and correlation tests. Twelve genes from the list of 73 genes also had a high correlation between glucose and gene expression as measured by Nanostring nCounter. The gene annotations indicated functions in protein degradation, nematode resistance, auxin transport, and gibberellin response. These 12 genes were used to build models for prediction of CIS using multiple linear regression. Nine linear models were constructed that used different combinations of the 12 genes. An F-box protein, cellulose synthase, and a putative Lax auxin transporter gene were most frequently used. The findings of this study demonstrate the utility of gene expression profiles in predictive diagnostics for severity of CIS.

  17. Induction and use of artificial mutants in sweet potato

    International Nuclear Information System (INIS)

    Marumine, Shokichi

    1984-01-01

    X-ray, ethylene imine, 32 P and 60 Co were used as mutagen for sweet potato mutation breeding and visible variations were observed for all mutagen. In the case of 60 Co irradiation, mutation rate of skin color is 0.5-1.3% based on cutting. Direction and variation of dry matter and tuber yield of mutants which were induced by 32 P and/or 60 Co irradiation showed more deteriorative variation than progressive variation but some induced mutant lines show same or superior characters than original line. In the case of 32 P irradiation to tuber, obstruction is not so much up to dese of 10,000 μci per tuber but treatment of 330 μci per cutting approximate to LD 50 . By tuber treatment with 60 Co gamma rays, suppression of sprouting occurred in dose of 30kR. Tendency to increase a variation was not observed at higher doses. 50-200 μci per cutting or 300-500 μci per tuber in 32 P treatment and 15 kR in 60 Co gamma-irradiation for tuber seemed to be optimum dosages. Hybrid seed of mutant selected for dry matter content was compared with that of original line and it was concluded that the variation of selected line was genetic. Mutant induced by 32 P and 60 Co treatment was used as a parental material and progeny of the cross was selected for practical characters. As a result, a line of higher starch yield with high resistance to pest and disease was selected and this line was used as parental material of further breeding. (author)

  18. Effect of application of Chlorpropham in sprouting of olluco tubers (Ullucus tuberosus L. under storage

    Directory of Open Access Journals (Sweden)

    Isaac Aliaga

    2011-06-01

    Full Text Available It was evaluated the effect of Chlorpropham on the sprouting of root’s tubers vegetables (Ullucus tuberosum L. Tarmeño variety during storage at 15 °C and RH of 85 %, the treatments were 0, 5, 10, 20 and 30 mg of Chlorpropham / kg of tubers, in a completely random design with 4 repetitions. After 60 days of storage the sprouts, length for the control without Chlorpropham was 9.63 cm and for the treatments with Chlorpropham from 0.22 to 0.71 cm after 90 days the length of the sprouts were kept the same and some of them began to dry up, except the control where increased in size from 15 to 20 cm. After 90 days of storage, weight loss for the treatments with Chlorpropham was averaged of 21 % and for the witness of 37.35 %. All Chlorpropham treatments showed a lower number of sprouts per tuber, lower length of buds, fewer number of tubers with sprouts and less weight loss, with a p <0.05, Duncan test showed don’t exist significant differences between treatments with Chlorpropham for the assessed variables, but significant differences with the control treatment

  19. Potential Regulatory Role of Gibberellic and Humic Acids in Sprouting of Chlorophytum borivilianum Tubers

    Directory of Open Access Journals (Sweden)

    Jaafar Juju Nakasha

    2014-01-01

    Full Text Available Tubers of safed musli (Chlorophytum borivilianum were immersed in three different concentrations of gibberellic acid (GA3 or humic acid (HA prior to planting. The highest concentration of GA3 (20 mg L-1 and all concentrations of HA (5, 10, and 15% appeared to hasten tuber sprouting and promote uniform sprouting pattern. The use of 20 mg L-1 GA3 or 15% HA successfully improved sprouting and mean sprouting time. Safed musli growth and development was improved through the increase in the number of leaves, total leaf area, leaf area index, and total fibrous root length. This directly influenced the number of new tubers formed. The use of 20 mg L-1 GA3 or 15% HA gave similar response with nonsignificant difference among them. However, due to the cost of production, the result from this study suggests that 15% HA should be used to obtain improved sprouting percentage, homogeneous stand establishment, efficient plant growth and development, and increased yield of safed musli.

  20. Potential regulatory role of gibberellic and humic acids in sprouting of Chlorophytum borivilianum tubers.

    Science.gov (United States)

    Nakasha, Jaafar Juju; Sinniah, Uma Rani; Puteh, Adam; Hassan, Siti Aishah

    2014-01-01

    Tubers of safed musli (Chlorophytum borivilianum) were immersed in three different concentrations of gibberellic acid (GA3) or humic acid (HA) prior to planting. The highest concentration of GA3 (20 mg L(-1)) and all concentrations of HA (5, 10, and 15%) appeared to hasten tuber sprouting and promote uniform sprouting pattern. The use of 20 mg L(-1) GA3 or 15% HA successfully improved sprouting and mean sprouting time. Safed musli growth and development was improved through the increase in the number of leaves, total leaf area, leaf area index, and total fibrous root length. This directly influenced the number of new tubers formed. The use of 20 mg L(-1) GA3 or 15% HA gave similar response with nonsignificant difference among them. However, due to the cost of production, the result from this study suggests that 15% HA should be used to obtain improved sprouting percentage, homogeneous stand establishment, efficient plant growth and development, and increased yield of safed musli.

  1. Glucosinolates in broccoli sprouts (Brassica oleracea var. italica) as conditioned by sulphate supply during germination.

    Science.gov (United States)

    Pérez-Balibrea, Santiago; Moreno, Diego A; García-Viguera, Cristina

    2010-10-01

    Sulphur (S) fertilization is essential for primary and secondary metabolism in cruciferous foods. Deficient, suboptimal, or excessive S affects the growth and biosynthesis of secondary metabolites in adult plants. Nevertheless, there is little information regarding the influence of S fertilization on sprouts and seedlings. An experiment was set up to evaluate the effect of S fertilization, supplied as K(2)SO(4) at 0, 15, 30, and 60 mg/L, on the glucosinolate content of broccoli sprouts during the germination course of 3, 6, 9, and 12 d after sowing. Glucosinolate concentration was strongly influenced by germination, causing a rapid increase during the first 3 d after sowing, and decreasing afterwards. The S supply increased aliphatic and total glucosinolate content at the end of the monitored sprouting period. S-treated sprouts, with S(15), S(30), and S(60) at 9 and 12 d after sowing presented enhanced glucosinolate content. Overall, both germination time and S fertilization were key factors in maximizing the bioactive health-promoting phytochemicals of broccoli. Practical Application: Germination with sulphate is a simple and inexpensive way to obtain sprouts that contain much higher levels of glucosinolates (health promoting compounds), than the corresponding florets from the same seeds.

  2. GDF10 Is a Signal for Axonal Sprouting and Functional Recovery after Stroke

    Science.gov (United States)

    Li, S; Nie, EH; Yin, Y; Benowitz, LI; Tung, S; Vinters, HV; Bahjat, FR; Stenzel-Poore, MP; Kawaguchi, R; Coppola, G; Carmichael, ST

    2016-01-01

    Stroke produces a limited process of neural repair. Axonal sprouting in cortex adjacent to the infarct is part of this recovery process, but the signal that initiates axonal sprouting is not known. Growth and Differentiation Factor 10 (GDF10) is induced in peri-infarct neurons in mouse, non-human primate and human. GDF10 promotes axonal outgrowth in vitro in mouse, rat and human neurons through TGFβRI/II signaling. Using pharmacogenetic gain and loss of function studies, GDF10 produces axonal sprouting and enhanced functional recovery after stroke; knocking down GDF10 blocks axonal sprouting and reduces recovery. RNA-seq from peri-infarct cortical neurons indicates that GDF10 downregulates PTEN and upregulates PI3 kinase signaling and induces specific axonal guidance molecules. Unsupervised genome-wide association analysis of the GDF10 transcriptome shows that it is not related to neurodevelopment but may partially overlap with other CNS injury patterns. GDF10 is a stroke-induced signal for axonal sprouting and functional recovery. PMID:26502261

  3. SPROUTS: a database for the evaluation of protein stability upon point mutation.

    Science.gov (United States)

    Lonquety, Mathieu; Lacroix, Zoé; Papandreou, Nikolaos; Chomilier, Jacques

    2009-01-01

    SPROUTS (Structural Prediction for pRotein fOlding UTility System) is a new database that provides access to various structural data sets and integrated functionalities not yet available to the community. The originality of the SPROUTS database is the ability to gain access to a variety of structural analyses at one place and with a strong interaction between them. SPROUTS currently combines data pertaining to 429 structures that capture representative folds and results related to the prediction of critical residues expected to belong to the folding nucleus: the MIR (Most Interacting Residues), the description of the structures in terms of modular fragments: the TEF (Tightened End Fragments), and the calculation at each position of the free energy change gradient upon mutation by one of the 19 amino acids. All database results can be displayed and downloaded in textual files and Excel spreadsheets and visualized on the protein structure. SPROUTS is a unique resource to access as well as visualize state-of-the-art characteristics of protein folding and analyse the effect of point mutations on protein structure. It is available at http://bioinformatics.eas.asu.edu/sprouts.html.

  4. Increased antioxidant activity and polyphenol metabolites in methyl jasmonate treated mung bean (Vigna radiata sprouts

    Directory of Open Access Journals (Sweden)

    Li LI

    Full Text Available Abstract Mung bean sprouts are a popular health food both in China and worldwide. We determined the optimal concentration of exogenous methyl jasmonate (MeJA for the promotion of the sprouting in mung beans (Vigna radiata. The 1,1-diphenyl-2- picrylhydrazyl radical (DPPH scavenging test showed that MeJA application resulted in significantly improved antioxidant capacity in the sprouts 72 h later. Measurement of total polyphenols in MeJA-treated beans from 0 to 168 h, using Folin–Ciocalteu colorimetry, showed that the polyphenols changing was significantly correlated with antioxidant activity. The main polyphenols isovitexin, kaempferol-3-O-rutinoside, daidzein, genistein, isoquercitrin, p-coumaric acid, and caffeic acid were quantified using high-performance liquid chromatography (HPLC/QqQ MS and partial least squares discriminant analysis (PLS-DA. MeJA promoted the production of polyphenols, metabolites, and antioxidants in the sprouts; therefore, its use may allow sprouts to be prepared more quickly or increase their nutritional value.

  5. Comparative study of potato cultivation through micropropagation ...

    African Journals Online (AJOL)

    A trial was carried out to evaluate the productivity of Solanum tuberosum L. cultivated through conventional farming and micropropagation method. Survival rate, biomass and tuber yield of both micropropagated and tuber propagated potatoes was evaluated. Survival percentages of potatoes were 90% for conventional ...

  6. Dormancy and growth vigour of seed potatoes

    NARCIS (Netherlands)

    Ittersum, van M.K.

    1992-01-01

    Dormancy is an important property of seed potatoes. Seed tubers planted too soon after their harvest do not produce plants because of dormancy, or produce low yields because of poor growth vigour. Potato tubers from the same cultivar vary in their duration of dormancy. The first aim of the

  7. Reinventing potato at the diploid level

    Science.gov (United States)

    The outcrossing polyploidy nature of cultivated potato has hindered the use of genomics resources to dissect the genetic basis of agronomically important traits. Reversion to the diploid level allows us to apply powerful tools toward this effort. Parthenogenesis generates diploid cultivated potato, ...

  8. Bio-ethanol production from waste potatoes

    Energy Technology Data Exchange (ETDEWEB)

    Kilpimaa, S.; Kuokkanen, T., Lassi, U. (Univ. of Oulu, Dept. of Chemistry (Finland)). email: toivo.kuokkanen@oulu.fi

    2009-07-01

    Ethanol can be used as an alternative fuel to gasoline. Bio-ethanol can be produced by fermentation from several renewable sources, such as from potatoes and corn. Globally, there is a growing interest for the production of ecologically sustainable bio-fuels. The target in the European Union is to compensate 5.75% of the fossil fuels which is used by traffic with biomass-based fuel by the year 2010 and 20% by the year 2020. The goal of United Nations climate conference in Bali is that industrial countries have to decrease total carbon dioxide effluents 30% by the year 2020. Potato-based bio-ethanol production utilizes waste potatoes as a raw material. Waste potatoes are produced as by-products in potato cultivation. The quality of waste potatoes is high enough for food production but the size is incorrect. In food potato industry a lot of solid potato mash is also formed which can be considered as raw material in bio-ethanol production

  9. Proximate analysis of Sweet Potato Toasted Granules

    African Journals Online (AJOL)

    Revd Dr Olaleye

    Sweet potato varieties with dark orange flesh have more beta carotene than those with light colored flesh and their increased cultivation is being encouraged in Africa where Vitamin A deficiency is a serious health problem. Sweet potato fries are a common preparation in most African homes. Its leaves are a common side ...

  10. Evaluation of Some Botanicals to Control Potato Tuber Moth ...

    African Journals Online (AJOL)

    ... in all treatments and non-significant differences were observed among them. From this study it can be conclude that Lantana camara, Eucalyptus globules and Pyretherum flowers could be used to protect seed potatoes from potato tuber moth damage in diffused light storage. Keywords: Seed Potato; Potato Tuber Moth; ...

  11. 7 CFR 318.13-25 - Sweet potatoes from Hawaii.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Sweet potatoes from Hawaii. 318.13-25 Section 318.13... Articles From Hawaii and the Territories § 318.13-25 Sweet potatoes from Hawaii. (a) Sweet potatoes may be... 5 Sweet potatoes may also be moved interstate from Hawaii with irradiation in accordance with § 305...

  12. Mean annual yield reductions of potato due to water deficits for Dutch weather conditions.

    NARCIS (Netherlands)

    Vos, J.; Groenwold, J.

    1987-01-01

    In The Netherlands drought causes occasional reductions in yield and quality of potatoes, especially on sandy soils. The mean annual yield loss determines the investments that can be made to prevent the losses. The mean annual loss is determined by the water deficit and the yield reduction per mm

  13. Biosynthesis and Accumulation of Sulphur Compounds in White Radish During the First Three Days of Sprouting

    Directory of Open Access Journals (Sweden)

    Maria Doinița Borș

    2015-11-01

    Full Text Available  Glucosinolates (GLs and S-methyl cysteine sulfoxide (SMCSO are natural sulphur containing phytochemicals. They are two of the most important bioactive compounds found in brassica vegetables, which are highly regarded for their health-promoting activity. In this study we have analysed the content of GLs and SMCSO in white radish, by an HPLC-MS method, in order to illustrate their biosynthesis and accumulation during the first 72 hours of sprouting. Total GLs content ranged between  54.17 and 126.86 µmol/g DW. There were eight GLs identified, in radish sprouts and around 94 % of them were aliphatic. Obvious differences, during the 72 hours of sprouting, were noticed in glucoraphenin and glucoraphasatin. S-methyl cysteine sulfoxide content ranged between 0.21 and 35.95 µmol/g DW. Our results revealed a negative strong correlation between GLs and SMCSO.

  14. Combined Effects of Blue and Ultraviolet Lights on the Accumulation of Flavonoids in Tartary Buckwheat Sprouts

    Directory of Open Access Journals (Sweden)

    Ji Hongbin

    2016-06-01

    Full Text Available The effects of blue and UV-A (365 nm/UV-C (254 nm or their combinations on the levels of total flavonoids, rutin, quercetin, phenylalanine ammonialyase (PAL, chalcone isomerase (CHI, rutin degrading enzymes (RDEs and 1,1-diphenyl-2-picrylhydrazyl (DPPH radical scavenging activity in tartary buckwheat sprouts were investigated in this study. The total flavonoids content in the tartary buckwheat sprouts irradiated with blue light followed by UV-C (BL+UV-C raised by 10%, compared with the opposite combination sequence (UV-C+BL. However, blue light did not show the same results when combined with UV-A, and their combinations on the accumulation of total flavonoids were still lower than that of UV-A/UV-C. Key enzymes (PAL, CHI and RDEs revealed a significant correlation with total flavonoids in tartary buckwheat sprouts.

  15. EFSA Panel on Biological Hazards (BIOHAZ); Scientific Opinion on the risk posed by Shiga toxinproducing Escherichia coli (STEC) and other pathogenic bacteria in seeds and sprouted seeds

    DEFF Research Database (Denmark)

    Hald, Tine

    , Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica have also been transmitted by sprouted seeds, albeit very rarely. Dry seed contaminated with bacterial pathogens has been identified as the most likely initial source of sprout-associated outbreaks; although other routes...

  16. Rattle tree (Albizia lebbeck Benth. effects on potato (Solanum tuberosum L. productivity on the Jos Plateau, Nigeria

    Directory of Open Access Journals (Sweden)

    Kareem Alasi Ibraheem

    2014-01-01

    Full Text Available Field experiment was conducted in the biotite-granite area (Alfisol of the Jos Plateau, Nigeria, consisting of five cropping seasons to determine the effects of the tree rows (under alley cropping and green manure of Albizia lebbeck Benth. (rattle tree on the yield / productivity of Solanum tuberosum L. (Irish potato. The experiment was a randomized complete block design comprising five treatments and three replicates. An early maturing potato variety (Bertita was employed, its pre-sprouted tubers were planted in the alleys (spaces between the tree rows of A. lebbeck two weeks after green manure was applied (5 and 10 t ha-1, pre and post experimental soil sample analyses and cultivation operations were carried out. Results indicated that the rattle tree had profound effect on the potato growth parameters (plant height, leaf count, collar girth (at P < 0.01 and yield indices (tuber count (P < 0.05 and tuber weight (P < 0.01 due to nutrients from the green manure and nitrogen fixation activities of the rattle tree. A. lebbeck green manure application at 10 t ha-1 in the alleys of A. lebbeck hedgerows emerged as the most effective treatment in terms of growth performance and optimal yield. Collar girth and leaf count of the potato accounted for 80.4 - 91.3% of the variation in yield (R2 = 0.804 - 0.913 with collar girth having the highest value (R2 =0.910 in rainy season (rain fed cropping, while leaf count had the highest value (R2=0.913 during the dry season (irrigated cropping.

  17. Detection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal.

    Science.gov (United States)

    Varanda, Carla M R; Santos, Susana J; Oliveira, Mônica D M; Clara, Maria Ivone E; Félix, Maria Rosário F

    2015-06-01

    Field sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA fractions were extracted from symptomatic leaves and used as templates in single and multiplex RT-PCR assays using previously described specific primers for each analyzed virus. The amplified reaction products for SPVC, SPV2 and SPFMV were of expected size, and direct sequencing of PCR products revealed that they correspond to the coat protein gene (CP) and showed 98%, 99% and 99% identity, respectively, to those viruses. Comparison of the CP genomic and amino acid sequences of the Portuguese viral isolates recovered here with those of ten other sequences of isolates obtained in different countries retrieved from the GenBank showed very few differences. The application of the RT-PCR assays revealed for the first time the presence of SPVC and SPFMV in the sweet potato crop in Portugal, the absence of SPVG and SPCSV in tested plants, as well as the occurrence of triple virus infections under field conditions.

  18. Contact-inhibited chemotaxis in de novo and sprouting blood-vessel growth.

    Directory of Open Access Journals (Sweden)

    Roeland M H Merks

    2008-09-01

    Full Text Available Blood vessels form either when dispersed endothelial cells (the cells lining the inner walls of fully formed blood vessels organize into a vessel network (vasculogenesis, or by sprouting or splitting of existing blood vessels (angiogenesis. Although they are closely related biologically, no current model explains both phenomena with a single biophysical mechanism. Most computational models describe sprouting at the level of the blood vessel, ignoring how cell behavior drives branch splitting during sprouting. We present a cell-based, Glazier-Graner-Hogeweg model (also called Cellular Potts Model simulation of the initial patterning before the vascular cords form lumens, based on plausible behaviors of endothelial cells. The endothelial cells secrete a chemoattractant, which attracts other endothelial cells. As in the classic Keller-Segel model, chemotaxis by itself causes cells to aggregate into isolated clusters. However, including experimentally observed VE-cadherin-mediated contact inhibition of chemotaxis in the simulation causes randomly distributed cells to organize into networks and cell aggregates to sprout, reproducing aspects of both de novo and sprouting blood-vessel growth. We discuss two branching instabilities responsible for our results. Cells at the surfaces of cell clusters attempting to migrate to the centers of the clusters produce a buckling instability. In a model variant that eliminates the surface-normal force, a dissipative mechanism drives sprouting, with the secreted chemical acting both as a chemoattractant and as an inhibitor of pseudopod extension. Both mechanisms would also apply if force transmission through the extracellular matrix rather than chemical signaling mediated cell-cell interactions. The branching instabilities responsible for our results, which result from contact inhibition of chemotaxis, are both generic developmental mechanisms and interesting examples of unusual patterning instabilities.

  19. The Involvement of Gibberellins in 1,8-Cineole-Mediated Inhibition of Sprout Growth in Russet Burbank Tubers

    Science.gov (United States)

    The involvement of gibberellins in 1,8-cineole-mediated inhibition of tuber sprout growth was investigated in non-dormant field- and greenhouse-grown tubers of Russet Burbank. Continuous exposure of tubers to cineole in the vapor-phase resulted in a dose-dependent inhibition of sprout growth. Comp...

  20. Long-term effects of burning on woody plant species sprouting on the False thornveld of Eastern Cape

    CSIR Research Space (South Africa)

    Ratsele, C

    2010-11-01

    Full Text Available Sprouting allows woody plant species to persist in a site after a wide range of disturbances (e.g. prolonged fire), where opportunities for seedling establishment are limited. A study to investigate long-term effects of fire sprouting of woody...

  1. Characteristics of soybean sprout locally cultivated in the Jeonju region, used for Bibimbap and Kongnamul-gukbap

    Directory of Open Access Journals (Sweden)

    Young-Eun Lee

    2015-06-01

    Conclusion: Soybean sprouts cultivated using Chinese soybeans in Jeonju were better in the overall acceptability than those grown in other regions because the Jeonju product contained two to three times less amino acids, such as leucine, tyrosine, and phenylalanine, than the other regional products, which tasted bitter. The cultivating water may affect the free amino-acid content of soybean sprouts and their taste.

  2. Impact of selenium supply on Se-methylselenocysteine and glucosinolate accumulation in selenium-biofortified Brassica sprouts.

    Science.gov (United States)

    Avila, Fabricio William; Yang, Yong; Faquin, Valdemar; Ramos, Silvio Junio; Guilherme, Luiz Roberto G; Thannhauser, Theodore W; Li, Li

    2014-12-15

    Brassica sprouts are widely marketed as functional foods. Here we examined the effects of Se treatment on the accumulation of anticancer compound Se-methylselenocysteine (SeMSCys) and glucosinolates in Brassica sprouts. Cultivars from the six most extensively consumed Brassica vegetables (broccoli, cauliflower, green cabbage, Chinese cabbage, kale, and Brussels sprouts) were used. We found that Se-biofortified Brassica sprouts all were able to synthesize significant amounts of SeMSCys. Analysis of glucosinolate profiles revealed that each Brassica crop accumulated different types and amounts of glucosinolates. Cauliflower sprouts had high total glucosinolate content. Broccoli sprouts contained high levels of glucoraphanin, a precursor for potent anticancer compound. Although studies have reported an inverse relationship between accumulation of Se and glucosinolates in mature Brassica plants, Se supply generally did not affect glucosinolate accumulation in Brassica sprouts. Thus, Brassica vegetable sprouts can be biofortified with Se for the accumulation of SeMSCys without negative effects on chemopreventive glucosinolate contents. Published by Elsevier Ltd.

  3. Effects of Yeast Polysaccharide on Growth and Flavonoid Accumulation in Fagopyrum tataricum Sprout Cultures

    Directory of Open Access Journals (Sweden)

    Gang Zhao

    2012-09-01

    Full Text Available The purpose of this study was to investigate the effects of yeast polysaccharide (YPS on growth and flavonoid accumulation in sprout cultures of Fagopyrum tataricum (tartary buckwheat. Without obvious change in the appearance of the sprouts, the exogenous YPS notably stimulated the production of functional metabolites in F. tataricum sprouts, and the stimulation effect was concentration-dependent. With 400 mg/L of YPS applied to the sprout cultures on day 6, the total rutin and quercentin content was effectively increased to 42.8 mg/gdw, or about 1.4-fold in comparison with the control of 31.2 mg/gdw. Feeding with 800 mg/L of YPS on day 9, the sprouts biomass was increased by about 8% compared to the control culture (0.99 gdw/100 sprouts versus 0.92 gdw/100 sprouts. Moreover, the present study revealed that the accumulation of these bioactive metabolites resulted from the stimulation of the phenylpropanoid pathway by YPS treatment. It could be an effective strategy for improving the functional quality of the F. tataricum sprouts provided with YPS.

  4. Study of selenocompounds from selenium-enriched culture of edible sprouts

    OpenAIRE

    Funes Collado, Virginia; Morell García, Albert; Rubio i Rovira, Roser; López Sánchez, José Fermín

    2013-01-01

    Selenium is recognised as an essential micronutrient for humans and animals. One of the main sources of selenocompounds in the human diet is vegetables. Therefore, this study deals with the Se species present in different edible sprouts grown in Se-enriched media. We grew alfalfa, lentil and soy in a hydroponic system amended with soluble salts, containing the same proportion of Se, in the form of Se(VI) and Se(IV). Total Se in the sprouts was determined by acidic digestion in a microwave sys...

  5. Isoenzymatic variability in wild potatoes

    Directory of Open Access Journals (Sweden)

    Rocha Beatriz Helena Gomes

    2001-01-01

    Full Text Available Two species of wild potato Solanum commersonii, subspecies commersonii and malmeanum, and S. chacoense, subspecies muelleri occur in southern Brazil. Their rusticity and easy adaptation to extreme climatic conditions make them valuable for breeding programs. The objective of this work was to analyze the isoenzymatic variability of 113 clones of wild potato subspecies. They were collected and maintained at Embrapa-Centro de Pesquisa Agropecuária de Clima Temperado, at Pelotas, RS, Brazil. Enzymes involved in energetic (group I or in peripherical (group II metabolism constituted the material used. Polyacrylamide horizontal gel electrophoresis was used to analyze peroxidase, aspartate transaminase, phosphoglucomutase and isocitrate dehydrogenase isoenzymes. Solanum spp. has considerable genetic variability for isoenzymatic patterns. Cluster analysis classified the clones into 51 subgroups, based on electrophoretic variants of group I enzymes, and into 89, when group II enzyme variants were added. Genotypic differentiation of S. chacoense muelleri in relation to S. commersonii commersonii and S. commersonii malmeanum is evident when expressed through similarity and cluster analysis.

  6. Risk Assessment of Salmonellosis from Consumption of Alfalfa Sprouts and Evaluation of the Public Health Impact of Sprout Seed Treatment and Spent Irrigation Water Testing.

    Science.gov (United States)

    Chen, Yuhuan; Pouillot, Régis; Santillana Farakos, Sofia M; Duret, Steven; Spungen, Judith; Fu, Tong-Jen; Shakir, Fazila; Homola, Patricia A; Dennis, Sherri; Van Doren, Jane M

    2018-01-16

    We developed a risk assessment of human salmonellosis associated with consumption of alfalfa sprouts in the United States to evaluate the public health impact of applying treatments to seeds (0-5-log 10 reduction in Salmonella) and testing spent irrigation water (SIW) during production. The risk model considered variability and uncertainty in Salmonella contamination in seeds, Salmonella growth and spread during sprout production, sprout consumption, and Salmonella dose response. Based on an estimated prevalence of 2.35% for 6.8 kg seed batches and without interventions, the model predicted 76,600 (95% confidence interval (CI) 15,400-248,000) cases/year. Risk reduction (by 5- to 7-fold) predicted from a 1-log 10 seed treatment alone was comparable to SIW testing alone, and each additional 1-log 10 seed treatment was predicted to provide a greater risk reduction than SIW testing. A 3-log 10 or a 5-log 10 seed treatment reduced the predicted cases/year to 139 (95% CI 33-448) or 1.4 (95% CI testing, a 3-log 10 or 5-log 10 seed treatment reduced the cases/year to 45 (95% CI 10-146) or testing with 20% coverage resulted in an estimated 92 (95% CI 22-298) cases/year. Analysis of alternative scenarios using different assumptions for key model inputs showed that the predicted relative risk reductions are robust. This risk assessment provides a comprehensive approach for evaluating the public health impact of various interventions in a sprout production system. © 2018 Society for Risk Analysis.

  7. Improvement of Constipation in Leukemia Patients Undergoing Chemotherapy Using Sweet Potato.

    Science.gov (United States)

    Zou, Jing-Ying; Xu, Yi; Wang, Xiao-Hua; Jiang, Qing; Zhu, Xia-Ming

    2016-01-01

    Leukemia patients undergoing chemotherapy commonly develop constipation, which is difficult to treat using routine methods. The aim of this study was to determine whether sweet potato can alleviate constipation in leukemia patients undergoing chemotherapy. Leukemia patients receiving their first chemotherapy were randomized to an intervention group (n = 57) or a control group (n = 63). The control and intervention groups were managed by using routine nursing methods and routine methods plus dietary sweet potato consumption (eating sweet potato 200 g/d, from admission to discharge), respectively. Related data regarding patients' defecation were collected on the second and fifth days after chemotherapy initiation, which included rates of constipation, frequency and duration of purgative usage, constipation status assessed by Rome III criteria, and scores on defecation satisfaction. On the second day, the rate of constipation and the rate of having first defecation within 24 hours after chemotherapy initiation were significantly improved in the intervention group, but the difference of the defecation satisfaction and "almost no loose stools without purgative use" in Rome III criteria were not significantly changed. On the fifth day, except for "the sensation of anorectal obstruction" and "requirement of manual assistance" in Rome III criteria, other items regarding defecation were significantly improved. This study demonstrates that sweet potato had a positive impact on the prevention of constipation and the defecation satisfaction in leukemia patients receiving their first chemotherapy. As sweet potato is inexpensive, abundant, tasty, and easy to prepare, it can be widely used in leukemia patients undergoing chemotherapy.

  8. SAYSOY- Space Apparatus to Yield SOY sprouts: Growing sprouts in a growth support system for experiments on unmanned platforms in space

    Science.gov (United States)

    De Micco, Veronica; Aronne, Giovanna; Scala, Michele; Eduardo, Pasquale; Haladich, Marco; Castagnolo, Dario; Fortezza, Raimondo

    2005-08-01

    The aim of this study was to design and develop an automatic growth support system for sprouts production in Space addressing a main biological requirement: seedling development had to occur completely under microgravity conditions from seed imbibition until chemical fixation of seedlings. This research is placed within the scenario of producing sprouts of soy, and other species, on board of Space platforms to integrate astronauts' diet with fresh food.The project SAYSOY was submitted within the Education programmes of ESA and was selected for the flight onboard of FOTON M2 satellite. The experiment is based on previous tests conducted on ground in simulated low-gravity conditions where emphasis was addressed to the effect of altered gravity on biological processes that affect nutritional value (metabolism of phenolics) and taste satisfaction (hydration of tissues, vascular differentiation and lignification affecting turgidity and softness of sprouts). The hardware was developed according to the specifications of the FOTON capsule and biological requirements in the sight of the planned analyses of biometrical anatomy on the recovered seedlings. The experiment SAYSOY was successfully conducted flying from May 31st to June 16th, 2005.

  9. Identification of irradiated potatoes by impedance

    International Nuclear Information System (INIS)

    Singh, Rita; Singh, Antaryami; Wadhawan, A.K.

    1997-01-01

    The electrical impedance of potatoes irradiated at 60, 90, 150 and 1000 Gy was measured using various frequencies of alternating current. The impedance of the irradiated potatoes was higher than the unirradiated potatoes particularly in the frequency range of 100 Hz to 10 kHz. The ratio of the impedance at 5 kHz to that at 50 Hz (Z5k/Z50) was found to be the best indicator for detection of radiation treatment. (author). 4 refs., 2 figs

  10. Oxylipin profile and antioxidant status of potato tubers during extended storage at room temperature.

    Science.gov (United States)

    Delaplace, Pierre; Rojas-Beltran, Jorge; Frettinger, Patrick; du Jardin, Patrick; Fauconnier, Marie-Laure

    2008-12-01

    Potato tubers (cv. Bintje) (Solanum tuberosum L.) were stored under extreme conditions at 20 degrees C for 350 days without sprout inhibitors in order to assess whether aging- and/or senescence-related processes occurred. Under these extreme storage conditions, multiple sprouting followed by the formation of daughter tubers occurs. At the same time, an increase in respiration intensity, as evidenced by cytochrome c oxidase activity (E.C. 1.9.3.1), is observed, leading to a potential increase in reactive oxygen species (ROS) production. As polyunsaturated fatty acids are priority targets of oxidative attacks, the damage to lipids was assessed by oxylipin profiling in both free and esterified forms. Oxylipin profiling showed a predominance of linoleic acid-derived oxylipins and of 9-hydroxy and 9-hydroperoxy fatty acids in both free and esterified forms. No significant accumulation of individual oxylipin was observed 350 days after harvest. To further understand the absence of lipid breakdown products accumulation, the main enzymatic and non-enzymatic antioxidants were assessed. Antioxidant enzyme activities [superoxide dismutase (E.C. 1.15.1.1), catalase (E.C. 1.11.1.6.), ascorbate peroxidase (E.C. 1.11.1.11)] were enhanced during the advanced phase of aging. The main non-enzymatic antioxidant compound, ascorbate, decreased markedly in the early stages of storage, followed by a slower decline. Total radical scavenging activity was also maintained at the end of the storage period. Our results indicate that the enhanced aging process occurring during storage at room temperature does not seem to be associated with the changes classically encountered during leaf senescence or seed aging and that the observed degenerative processes do not surpass the protective potential of the tubers.

  11. Distinct activities of Bartonella henselae type IV secretion effector proteins modulate capillary-like sprout formation.

    Science.gov (United States)

    Scheidegger, F; Ellner, Y; Guye, P; Rhomberg, T A; Weber, H; Augustin, H G; Dehio, C

    2009-07-01

    The zoonotic pathogen Bartonella henselae (Bh) can lead to vasoproliferative tumour lesions in the skin and inner organs known as bacillary angiomatosis and bacillary peliosis. The knowledge on the molecular and cellular mechanisms involved in this pathogen-triggered angiogenic process is confined by the lack of a suitable animal model and a physiologically relevant cell culture model of angiogenesis. Here we employed a three-dimensional in vitro angiogenesis assay of collagen gel-embedded endothelial cell (EC) spheroids to study the angiogenic properties of Bh. Spheroids generated from Bh-infected ECs displayed a high capacity to form sprouts, which represent capillary-like projections into the collagen gel. The VirB/VirD4 type IV secretion system and a subset of its translocated Bartonella effector proteins (Beps) were found to profoundly modulate this Bh-induced sprouting activity. BepA, known to protect ECs from apoptosis, strongly promoted sprout formation. In contrast, BepG, triggering cytoskeletal rearrangements, potently inhibited sprouting. Hence, the here established in vitro model of Bartonella- induced angiogenesis revealed distinct and opposing activities of type IV secretion system effector proteins, which together with a VirB/VirD4-independent effect may control the angiogenic activity of Bh during chronic infection of the vasculature.

  12. Effects of growth media and hormones on the sprouting and rooting ...

    African Journals Online (AJOL)

    Massularia acuminata is both a medicinal and economic tree species. Despite its usefulness, adequate attention has not been given to its propagation and cultivation. Therefore, this study investigated the effect of growth media and hormonal concentration on the sprouting and rooting of M. acuminata stem cuttings using ...

  13. Study of Active Ingredients in Black Soybean Sprouts and Their Safety in Cosmetic Use

    Directory of Open Access Journals (Sweden)

    Yinmao Dong

    2012-10-01

    Full Text Available Active ingredients in different lengths of black soybean sprouts were extracted with water. Concentrations of the main proteins and polysaccharides were determined by the Forint phenol assay and phenol-sulfuric acid assay, respectively. Anti-oxidizing capacities of the extracts were measured in vitro using the DPPH scavenging test and whitening capacity was measured in vitro using the tyrosinase inhibition test. The effects of the bean sprout extracts on human skin fibroblasts damnified by H2O2 were studied using an MTT colorimetric assay. The safety of the extracts was determined using the red blood cell (RBC test, chick chorioallantoic membrane (CAM assay and human patch test. Results show that DPPH radical scavenging rates at different shoot lengths were all greater than 95%, while the tyrosinase inhibition capacity of the extracts reached 98%. Hemolysis rate in all extracts were lower than 10%, below the 20% regulatory limit for the RBC test. No signs of allergic reactions were observed in the human patch tests. The optimum extract was obtained from bean sprouts grown to 0.5 cm. Extracts of black bean sprouts are safe and can be used as additives in anti-aging and whitening cosmetic products.

  14. Chloroxyanion residue on seeds and sprouts after chlorine dioxide sanitation of alfalfa seed

    Science.gov (United States)

    The effects of a 6-h chlorine dioxide sanitation of alfalfa seed (0, 50, 100, and 200 mg/kg seed) on total coliform bacteria, seed germination, and on the presence of chlorate and perchlorate residues in seed rinse, seed soak, and in alfalfa sprouts was determined. Chlorate residues in 20000 ppm cal...

  15. MG SPROUTS: A Project-in-a-Box Approach to Educational Programming

    Science.gov (United States)

    Dorn,Sheri; Slagle, Krissy

    2016-01-01

    MG SPROUTS was developed as a "project-in-a-box" program, a self-contained educational programming tool for Extension agents working with master gardener Extension volunteers (MGEVs). The program design incorporates programmatic materials and project management materials and follows best management practices for volunteer management. MG…

  16. Perforant path lesioning induces sprouting of CA3-associated fibre systems in mouse hippocampal formation

    DEFF Research Database (Denmark)

    Drøjdahl, Nina; Hegelund, Iørn V; Poulsen, Frantz R

    2002-01-01

    mice. We found that lesioning led to translaminar sprouting of Timm stained regio inferior hippocampus (CA3)-associated fibre systems into the denervated termination zones of the CA3 and dentate gyrus, from the adjacent non-denervated stratum radiatum of CA3. Differences were seen in the Timm staining...

  17. Oak stump-sprout vigor and Armillaria infection after clearcutting in southeastern Missouri, USA

    Science.gov (United States)

    Christopher A. Lee; Daniel C. Dey; Rose-Marie Muzika

    2016-01-01

    Armillaria spp. occur widely in Missouri mixed-oak ecosystems. In order to better understand the ecology and management of this pathogen and its effects on oak coppice, we observed a transect of 150 stumps after clearcutting in southeastern Missouri, noting Armillaria infection and oak sprout demography one year and seven years...

  18. Vasohibin inhibits angiogenic sprouting in vitro and supports vascular maturation processes in vivo

    International Nuclear Information System (INIS)

    Kern, Johann; Steurer, Michael; Gastl, Günther; Gunsilius, Eberhard; Untergasser, Gerold

    2009-01-01

    The murine homologue of human vasohibin (mVASH1), a putative antiangiogenic protein, was investigated for its effects on in vitro and in vivo angiogenesis. Cell growth and migration were analyzed in murine fibroblasts, smooth muscle cells and endothelial cells. Angiogenic sprouting was studied in human umbilical vein endothelial cells (HUVECs) in the spheroid sprouting assay. In vivo effects on blood vessel formation were investigated in the chorioallantoic membrane (CAM) assay and in the C57BL/6 melanoma xenograft model. Purified murine and human VASH1 protein induced apoptosis of murine fibroblasts in vitro, but not of vascular aortic smooth muscle cells (AoSMC) or endothelial cells. Adenoviral overexpression of murine and human VASH1 inhibited capillary sprouting of HUVECs in the spheroid assay. Administration of recombinant murine and human VASH1 inhibited growth of large vessels in the CAM assay and promoted the formation of a dense, fine vascular network. Murine VASH1-overexpressing B16F10 melanomas displayed a reduction in large vessels and vascular area. Moreover, tumors showed more microvessels that stained positive for the mural cell markers α-smooth muscle cell actin (ASMA) and proteoglycan (NG2). Our data imply that murine VASH1 causes angiogenic remodelling by inhibiting angiogenic sprouting and large vessel growth, thereby supporting the formation of a vascular bed consisting predominantly of mature microvessels

  19. Root-sprouting in myco-heterotrophic plants: prepackaged symbioses or overcoming meristem limitation?

    Czech Academy of Sciences Publication Activity Database

    Klimešová, Jitka

    2007-01-01

    Roč. 173, - (2007), s. 8-10 ISSN 0028-646X Institutional research plan: CEZ:AV0Z60050516 Keywords : root sprouting * bud bank * Myco-heterotrophic plants Subject RIV: EF - Botanics Impact factor: 5.249, year: 2007

  20. Analysis and Antioxidant Activity of Extracts from Broccoli (Brassica oleracea L.) Sprouts.

    Science.gov (United States)

    Jang, Hae Won; Moon, Joon-Kwan; Shibamoto, Takayuki

    2015-02-04

    Samples prepared from fresh broccoli (Brassica oleracea L.) sprouts by water distillation or freeze-drying were examined for antioxidant activity using three assays. All samples exhibited dose-dependent antioxidant activity. The antioxidant activity ranged from 74.48 ± 0.46% (less volatile sample) to 93.2 ± 0.2% (dichloromethane extract sample) at the level of 500 μg/mL. Both dichloromethane extract samples from a water distillate of broccoli sprouts and freeze-dried broccoli sprouts showed potent antioxidant activity, which was comparable to that of BHT. Among the 43 compounds positively identified by gas chromatography-mass spectrometry, 5-methylthiopentylnitrile (31.64 μg/g) was found in the greatest concentration, followed by 4-methylthiobutylisothiocyanate (14.55 μg/g), 4-methylthiobutylnitrile (10.63 μg/g), 3-methylthiopropylisothiocyanate (3.00 μg/g), and 4-methylpentylisothiocyanate (2.48 μg/g). These isothiocyanates are known to possess antioxidant properties. Possible phenolic antioxidants found are 4-(1-methylpropyl)phenol (0.012 μg/g), 4-methylphenol (0.159 μg/g), and 2-methoxy-4-vinylphenol (0.009 μg/g). The present study demonstrates that broccoli sprouts are a good source of natural antioxidants.

  1. Chopper GEN2 + Glyphosate efficacy for height classes of hardwood sprouts recolonizing six clearcut pine sites

    Science.gov (United States)

    Jimmie Yeiser; Andrew Ezell

    2015-01-01

    The purpose of this study was to assess sprout size as a determinant of subsequent control by a standard, single rate of imazapyr +glyphosate applied during site preparation. All study sites were in the hilly upper coastal plain of Mississippi (Winston or Oktibbeha Counties) or Louisiana (Sabine or Winn Parishes) and supported loblolly pine (Pinus taeda L.) plantations...

  2. Stump sprouting of northern pin oak on nutrient-poor sandy soils in central Wisconsin

    Science.gov (United States)

    Kevin M. Schwartz; Michael C. Demchik

    2013-01-01

    Coppice with two to three reserve trees per acre is the generally accepted practice (GAP) for rotating oak stands on nutrient-poor, sandy sites (colloquially called "scrub oak sites") in Wisconsin. The future stocking of the stand is therefore dependent predominantly on stump sprouts with varying levels of contribution from advance regeneration. Two groups of...

  3. Abundance, diversity and community composition of free-living protozoa on vegetable sprouts.

    Science.gov (United States)

    Chavatte, N; Lambrecht, E; Van Damme, I; Sabbe, K; Houf, K

    2016-05-01

    Interactions with free-living protozoa (FLP) have been implicated in the persistence of pathogenic bacteria on food products. In order to assess the potential involvement of FLP in this contamination, detailed knowledge on their occurrence, abundance and diversity on food products is required. In the present study, enrichment and cultivation methods were used to inventory and quantify FLP on eight types of commercial vegetable sprouts (alfalfa, beetroot, cress, green pea, leek, mung bean, red cabbage and rosabi). In parallel, total aerobic bacteria and Escherichia coli counts were performed. The vegetable sprouts harbored diverse communities of FLP, with Tetrahymena (ciliate), Bodo saltans and cercomonads (flagellates), and Acanthamoeba and Vannella (amoebae) as the dominant taxa. Protozoan community composition and abundance significantly differed between the sprout types. Beetroot harbored the most abundant and diverse FLP communities, with many unique species such as Korotnevella sp., Vannella sp., Chilodonella sp., Podophrya sp. and Sphaerophrya sp. In contrast, mung bean sprouts were species-poor and had low FLP numbers. Sampling month and company had no significant influence, suggesting that seasonal and local factors are of minor importance. Likewise, no significant relationship between protozoan community composition and bacterial load was observed. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Natural seedlings and sprouts after regeneration cuttings in old-growth redwood

    Science.gov (United States)

    Kenneth N. Boe

    1975-01-01

    Natural regeneration of harvested old-growth stands of redwood (Sequoia sempervirens) is one way to start a new forest that is needed quickly for continuous timber production. Natural seedlings and sprouts developing after stands were cut were studied on the Redwood Experimental Forest, northern California. Three types of regeneration cuttings were...

  5. Effect of Soybean Sprouting and Beta-Glucanase Treatment of Wet ...

    African Journals Online (AJOL)

    Effect of Soybean Sprouting and Beta-Glucanase Treatment of Wet Milled Soybean on the Chemical Properties of Soymilk from Different Varieties of Soybean. ... Sugars were also qualitatively and quantitatively analyzed with thin layer chromatography and spectrophotometry respectively. Proximate composition and dietary ...

  6. Axonal sprouting regulates myelin basic protein gene expression in denervated mouse hippocampus

    DEFF Research Database (Denmark)

    Jensen, M B; Poulsen, F R; Finsen, B

    2000-01-01

    radiatum of CA3 and the dentate hilus, which display axonal sprouting but no degenerative changes or microglial activation, and (2) the outer part of the molecular layer of the fascia dentata, and in stratum moleculare of CA3 and stratum lacunosum-moleculare of CA1, areas that display dense anterograde...

  7. Cell autonomy of HIF effects in Drosophila: tracheal cells sense hypoxia and induce terminal branch sprouting.

    Science.gov (United States)

    Centanin, Lázaro; Dekanty, Andrés; Romero, Nuria; Irisarri, Maximiliano; Gorr, Thomas A; Wappner, Pablo

    2008-04-01

    Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-alpha homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting.

  8. Production of Bioethanol from Waste Potato

    Directory of Open Access Journals (Sweden)

    Merve Duruyurek

    2015-02-01

    Full Text Available Using primary energy sources in World as fossil fuels, causes air pollution and climate change. Because of these reasons, people looking for renewable energy suppliers which has less carbondioxide and less pollution. Carbon in biofuels is producing from photosynthesis. For this, burning biofuels don’t increase carbondioxide in atmosphere. Scientists predict that plants with high carbonhydrate and protein contents are 21. centuries biofuels. Potatoes are producing over 280 million in whole world and Turkey is 6th potato producer. Turkey produces 5250000 tonne of potatoes. Approximately 20% of potatoes are waste in Niğde. Our study aimed to produce bioethanol from Solanum tuberosum by using the yeast Saccharomyces cerevisiae. As a result renewable energy sources can be produced from natural wastes.

  9. Analysis of Seed Potato Systems in Ethiopia

    NARCIS (Netherlands)

    Hirpa, A.; Meuwissen, M.P.M.; Tesfaye, A.; Lommen, W.J.M.; Oude Lansink, A.G.J.M.; Tsegaye, A.; Struik, P.C.

    2010-01-01

    This study aimed to analyze the seed potato systems in Ethiopia, identify constraints and prioritize improvement options, combining desk research, rapid appraisal and formal surveys, expert elicitation, field observations and local knowledge. In Ethiopia, informal, alternative and formal seed

  10. Contribution of Noncolonizing Aphids to Potato Virus Y Prevalence in Potato in Idaho.

    Science.gov (United States)

    Mondal, Shaonpius; Wenninger, Erik J; Hutchinson, Pamela J S; Weibe, Monica A; Eigenbrode, Sanford D; Bosque-Pérez, Nilsa A

    2016-12-01

    Potato virus Y (PVY) is a major concern for potato production in the United States given its impact on both crop quality and yield. Although green peach aphid, Myzus persicae (Sulzer), is the most efficient PVY vector, it may be less abundant in potato-growing areas of Idaho relative to non-potato-colonizing aphid vectors of PVY that may disperse from nearby cereal fields and other crops. A field study was conducted during 2012-2013 to examine if noncolonizing aphids disperse to nearby potato fields as cereal crops dry down before harvest. The aphid fauna was sampled weekly in four different potato fields in south-central and southeastern Idaho using yellow sticky traps and yellow pan traps. Potato fields were chosen with an adjacent cereal field such that the prevailing westerly wind would facilitate aphid dispersal from cereal fields to potato. Non-potato-colonizing aphids sampled included 10 cereal aphid species, the most abundant of which were Rhopalosiphum padi L. and Metopolophium dirhodum (Walker). More than 35 species from noncereal hosts also were found. Overall, green peach aphid abundance was relatively low, ranging from 0.5-2.5% of the total aphid capture between years and among fields. In both years and all locations, cereal aphid abundance peaked in mid- to late July (cereal ripening stage) and decreased thereafter as cereal crops dried. PVY prevalence in the potato fields increased following these increases in aphid abundance. This study suggests that cereal aphids and other noncolonizing aphids are important contributors to PVY prevalence in potato in southern Idaho. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  11. Development of molecular resistance in potato against potato leaf roll virus and potato virus Y through Agrobacterium-mediated double transgenesis

    Science.gov (United States)

    Potato leafroll virus (PLRV) and potato virus Y (PVY) are the two major viral problems for the potato production all over the world. Transgenic approaches involving the expression of viral genes are being developed to provide protection for plants against viral diseases. The purpose of this study w...

  12. Effect of potato used as a trap crop on potato cyst nematodes and other soil pathogens and on the growth of a subsequent main potato crop

    NARCIS (Netherlands)

    Scholte, K.

    2000-01-01

    A field experiment in which main-crop potatoes were grown every other year was conducted on a sandy soil from 1994 to 1999. The aim of the experiment was to control soil-borne pathogens of potato with ecologically sound methods. Potato grown as a trap crop from the end of April to the end of June (8

  13. Well-known quorum sensing inhibitors do not affect bacterial quorum sensing-regulated bean sprout spoilage.

    Science.gov (United States)

    Rasch, M; Rasmussen, T B; Andersen, J B; Persson, T; Nielsen, J; Givskov, M; Gram, L

    2007-03-01

    To investigate the potential of quorum sensing inhibitors (QSI) as food preservative agents in a food product, where bacterial spoilage is controlled by quorum sensing (QS). The effects of well-known QSI were tested on spoilage phenotypes and on QS-regulated genes of a bean sprout spoiling bacterial isolate (Pectobacterium A2JM) in laboratory substrates and in a bean sprout model system. The acylated homoserine lactones (AHL) analogues PenS-AHL and HepS-AHL decreased the specific protease activity of Pectobacterium A2JM in broth but did not reduce the expression of a QS-regulated secretion protein, and were without effect on soft rot of bean sprouts. The QSI ProS-AHL, furanone C-30, patulin, penicillic acid and 4-nitropyridine-N-oxide did not have any effect on protease activity, on gene expression or bean sprout appearance at nongrowth inhibitory concentrations. Extracts from garlic and bean sprouts induced the QS system of Pectobacterium in bean sprouts and a broth system, respectively. Among the several well-known QSI compounds, only PenS-AHL and HepS-AHL, inhibited QS-regulated protease activity of Pectobacterium A2JM in broth cultures, but had no effect on bean sprout spoilage. The QSI compounds must be selected in the specific system in which they are to function and they cannot easily be transferred from one QS system to another.

  14. Seed viability and functional properties of broccoli sprouts during germination and postharvest storage as affected by irradiation of seeds.

    Science.gov (United States)

    Waje, Catherine K; Jun, So-Yun; Lee, Yeon-Kyung; Moon, Kwang-Deog; Choi, Yong Hee; Kwon, Joong-Ho

    2009-06-01

    The viability of broccoli seeds and functional properties, such as ascorbic acid, carotenoid, chlorophyll, and total phenol contents, of broccoli sprouts grown from irradiated seeds were evaluated. The seeds were irradiated using electron beam and gamma ray at doses up to 8 kGy. High germination percentages (>90%) were observed in seeds irradiated at or =6 kGy resulted in curling of the sprout roots. Germinated seeds contained higher amounts of nutrients than raw seeds but the nutritional quality of sprouts decreased during postharvest storage. Radiation treatment hampered the growth of irradiated seeds resulting in underdeveloped sprouts with decreased ascorbic acid, carotenoid, and chlorophyll contents. In addition, the decrease in functional content of sprouts was more substantial in samples grown from high-dose (5 kGy) irradiated seeds than that of the low-dose (1 kGy) treated ones. Seed irradiation did not negatively affect the total phenol content of sprouts. In general, electron beam and gamma irradiation of broccoli seeds showed similar effects on the viability and functional properties of sprouts.

  15. The influence of selenium addition during germination of Brassica seeds on health-promoting potential of sprouts.

    Science.gov (United States)

    Piekarska, Anna; Kołodziejski, Dominik; Pilipczuk, Tadeusz; Bodnar, Małgorzata; Konieczka, Piotr; Kusznierewicz, Barbara; Hanschen, Franziska S; Schreiner, Monika; Cyprys, Joanna; Groszewska, Milena; Namieśnik, Jacek; Bartoszek, Agnieszka

    2014-09-01

    The correlation among selenium uptake, the content of bioactive compounds in sprouts, and biological activities triggered in cultured human cells by sprout extracts was investigated. Seeds of Brassica crops and rye were treated with SeO2 water solution. The selenium levels in sprouts increased from 1.0-4.1 to 53.3-382 μg/g dw with no influence on plant physiology according to the indices used. Neither the composition of glucosinolates (GL) in Brassica sprouts nor the myrosinase activity nor the composition of GL breakdown lipophilic products were significantly affected. In all Brassica sprouts, conversion to health-promoting isothiocyanates (ITC) and indoles corresponded to only 1% of total GLs. Low ITC concentration may explain observed lack of induction of glutathione S-transferases (GST) and quinone oxidoreductase (NQO) detoxifying enzymes in HT29 cells exposed to sprout extracts. The insignificant impact on cell growth and genome function suggests that Brassica sprouts may be safe vehicle of selenium to combat its dietary deficiency.

  16. Soybean (Glycine max L. Merr.) sprouts germinated under red light irradiation induce disease resistance against bacterial rotting disease.

    Science.gov (United States)

    Dhakal, Radhika; Park, Euiho; Lee, Se-Weon; Baek, Kwang-Hyun

    2015-01-01

    Specific wavelengths of light can exert various physiological changes in plants, including effects on responses to disease incidence. To determine whether specific light wavelength had effects on rotting disease caused by Pseudomonas putida 229, soybean sprouts were germinated under a narrow range of wavelengths from light emitting diodes (LEDs), including red (650-660), far red (720-730) and blue (440-450 nm) or broad range of wavelength from daylight fluorescence bulbs. The controls were composed of soybean sprouts germinated in darkness. After germination under different conditions for 5 days, the soybean sprouts were inoculated with P. putida 229 and the disease incidence was observed for 5 days. The sprouts exposed to red light showed increased resistance against P. putida 229 relative to those grown under other conditions. Soybean sprouts germinated under red light accumulated high levels of salicylic acid (SA) accompanied with up-regulation of the biosynthetic gene ICS and the pathogenesis- related (PR) gene PR-1, indicating that the resistance was induced by the action of SA via de novo synthesis of SA in the soybean sprouts by red light irradiation. Taken together, these data suggest that only the narrow range of red light can induce disease resistance in soybean sprouts, regulated by the SA-dependent pathway via the de novo synthesis of SA and up-regulation of PR genes.

  17. Soybean (Glycine max L. Merr. sprouts germinated under red light irradiation induce disease resistance against bacterial rotting disease.

    Directory of Open Access Journals (Sweden)

    Radhika Dhakal

    Full Text Available Specific wavelengths of light can exert various physiological changes in plants, including effects on responses to disease incidence. To determine whether specific light wavelength had effects on rotting disease caused by Pseudomonas putida 229, soybean sprouts were germinated under a narrow range of wavelengths from light emitting diodes (LEDs, including red (650-660, far red (720-730 and blue (440-450 nm or broad range of wavelength from daylight fluorescence bulbs. The controls were composed of soybean sprouts germinated in darkness. After germination under different conditions for 5 days, the soybean sprouts were inoculated with P. putida 229 and the disease incidence was observed for 5 days. The sprouts exposed to red light showed increased resistance against P. putida 229 relative to those grown under other conditions. Soybean sprouts germinated under red light accumulated high levels of salicylic acid (SA accompanied with up-regulation of the biosynthetic gene ICS and the pathogenesis- related (PR gene PR-1, indicating that the resistance was induced by the action of SA via de novo synthesis of SA in the soybean sprouts by red light irradiation. Taken together, these data suggest that only the narrow range of red light can induce disease resistance in soybean sprouts, regulated by the SA-dependent pathway via the de novo synthesis of SA and up-regulation of PR genes.

  18. Mutations in cauliflower and sprout broccoli grown from seeds flown in space

    Science.gov (United States)

    Wu, Hong; Huang, Congli; Zhang, Keping; Sun, Yeqing

    2010-11-01

    Cauliflower and sprout broccoli are widely planted vegetables particularly in Fujian Province, China. To study the mutation in these two types of vegetables induced from spaceflight, we flew the seeds on the 20th Chinese recoverable satellite which orbited the Earth for 18 days. After returning to the Earth, the cauliflower seeds were planted for two generations and the sprout broccoli seeds for one generation at the Xiamen Agriculture Research Institute. Of the 12 cauliflowers planted for the first generation, two showed significant phenotypical changes in both the size of the plant and the weight of the flower head. In addition, most of the space flown plants were found to be resistant to the black rot attack in the field. Cauliflowers planted for the second generation from the seeds in one of the two plants that displayed phenotypical changes in the first generation showed similar mutations. For the first generation of sprout broccoli, the rate of emergence from the flown seeds was lower than that of the control by 30%. No significant changes in the phenotype between the sprout broccolis planted from the flown seeds and the control were observed except one of the mutated sprout broccolis showed a change in the appearance in the lesser bud of the chief flower head. Results of the study demonstrated that DNA damages in some of the genes may have occurred in the seeds flown in space, and some of the changes in the genes may have inherited from the first to the second generation. The improved resistance to the black rot attack and increased size of the flower head are apparently beneficial.

  19. Ethylene and 1-methylcyclopropene differentially regulate gene expression during onion sprout suppression.

    Science.gov (United States)

    Cools, Katherine; Chope, Gemma A; Hammond, John P; Thompson, Andrew J; Terry, Leon A

    2011-07-01

    Onion (Allium cepa) is regarded as a nonclimacteric vegetable. In onions, however, ethylene can suppress sprouting while the ethylene-binding inhibitor 1-methylcyclopropene (1-MCP) can also suppress sprout growth; yet, it is unknown how ethylene and 1-MCP elicit the same response. In this study, onions were treated with 10 μL L(-1) ethylene or 1 μL L(-1) 1-MCP individually or in combination for 24 h at 20°C before or after curing (6 weeks) at 20°C or 28°C and then stored at 1°C. Following curing, a subset of these same onions was stored separately under continuous air or ethylene (10 μL L(-1)) at 1°C. Onions treated with ethylene and 1-MCP in combination after curing for 24 h had reduced sprout growth as compared with the control 25 weeks after harvest. Sprout growth following storage beyond 25 weeks was only reduced through continuous ethylene treatment. This observation was supported by a higher proportion of down-regulated genes characterized as being involved in photosynthesis, measured using a newly developed onion microarray. Physiological and biochemical data suggested that ethylene was being perceived in the presence of 1-MCP, since sprout growth was reduced in onions treated with 1-MCP and ethylene applied in combination but not when applied individually. A cluster of probes representing transcripts up-regulated by 1-MCP alone but down-regulated by ethylene alone or in the presence of 1-MCP support this suggestion. Ethylene and 1-MCP both down-regulated a probe tentatively annotated as an ethylene receptor as well as ethylene-insensitive 3, suggesting that both treatments down-regulate the perception and signaling events of ethylene.

  20. Ethylene and 1-Methylcyclopropene Differentially Regulate Gene Expression during Onion Sprout Suppression1[W][OA

    Science.gov (United States)

    Cools, Katherine; Chope, Gemma A.; Hammond, John P.; Thompson, Andrew J.; Terry, Leon A.

    2011-01-01

    Onion (Allium cepa) is regarded as a nonclimacteric vegetable. In onions, however, ethylene can suppress sprouting while the ethylene-binding inhibitor 1-methylcyclopropene (1-MCP) can also suppress sprout growth; yet, it is unknown how ethylene and 1-MCP elicit the same response. In this study, onions were treated with 10 μL L−1 ethylene or 1 μL L−1 1-MCP individually or in combination for 24 h at 20°C before or after curing (6 weeks) at 20°C or 28°C and then stored at 1°C. Following curing, a subset of these same onions was stored separately under continuous air or ethylene (10 μL L−1) at 1°C. Onions treated with ethylene and 1-MCP in combination after curing for 24 h had reduced sprout growth as compared with the control 25 weeks after harvest. Sprout growth following storage beyond 25 weeks was only reduced through continuous ethylene treatment. This observation was supported by a higher proportion of down-regulated genes characterized as being involved in photosynthesis, measured using a newly developed onion microarray. Physiological and biochemical data suggested that ethylene was being perceived in the presence of 1-MCP, since sprout growth was reduced in onions treated with 1-MCP and ethylene applied in combination but not when applied individually. A cluster of probes representing transcripts up-regulated by 1-MCP alone but down-regulated by ethylene alone or in the presence of 1-MCP support this suggestion. Ethylene and 1-MCP both down-regulated a probe tentatively annotated as an ethylene receptor as well as ethylene-insensitive 3, suggesting that both treatments down-regulate the perception and signaling events of ethylene. PMID:21593215

  1. Inhibition of Salmonella enterica by plant-associated pseudomonads in vitro and on sprouting alfalfa seed.

    Science.gov (United States)

    Fett, William F

    2006-04-01

    Foodborne illness due to the consumption of contaminated raw or lightly cooked sprouts is a continuing food safety concern. In this study, we tested several plant-associated pseudomonads for their ability to inhibit the growth of Salmonella enterica both in vitro and in situ. An agar spot bioassay method was used with three different media. Only Pseudomonas fluorescens 2-79 produced clear zones of inhibition when tested against five serovars of S. enterica, and activity was dependent on media type and serovar. The antibiosis by derivative strains of P. fluorescens 2-79 defective in the production of phenazine-1-carboxylic acid and fluorescent siderophore was not reduced, indicating that these known antimicrobial metabolites were not responsible for the inhibition observed in our studies. However, mutants defective in the regulatory gene gacS (global antibiotic and cyanide control) were severely reduced in inhibitory activity. In tryptic soy broth, the control cultures of a cocktail of S. enterica strains reached approximately 10 log CFU/ml by 24 h but, when coinoculated with P. fluorescens 2-79, reached only approximately 5 log CFU/ml. The addition of P. fluorescens 2-79 to the seed soak water prior to the germination of alfalfa seed previously inoculated with a cocktail of S. enterica strains led to an average reduction of 5 log CFU/g at 6 days of sprouting without an adverse effect on sprout yield or appearance. Time course studies indicated that S. enterica outgrowth was controlled on days 1 through 6 of sprouting. Competitive exclusion as a potential food safety intervention for seed sprouts merits further study.

  2. Effects of Polysaccharide Elicitors from Endophytic Fusarium oxysporum Fat9 on the Growth, Flavonoid Accumulation and Antioxidant Property of Fagopyrum tataricum Sprout Cultures

    Directory of Open Access Journals (Sweden)

    Lingyun Zhong

    2016-11-01

    Full Text Available The purpose of this study was to evaluate the effects of four different fungal polysaccharides, named water-extracted mycelia polysaccharide (WPS, sodium hydroxide-extracted mycelia polysaccharide (SPS, hydrochloric-extracted mycelia polysaccharide (APS, and exo-polysaccharide (EPS obtained from the endophytic Fusarium oxysporum Fat9 on the sprout growth, flavonoid accumulation, and antioxidant capacity of tartary buckwheat. Without visible changes in the appearance of the sprouts, the exogenous polysaccharide elicitors strongly stimulated sprout growth and flavonoid production, and the stimulation effect was closely related with the polysaccharide (PS species and its treatment dosage. With application of 200 mg/L of EPS, 200 mg/L of APS, 150 mg/L of WPS, or 100 mg/L of SPS, the total rutin and quercetin yields of buckwheat sprouts were significantly increased to 41.70 mg/(100 sprouts, 41.52 mg/(100 sprouts, 35.88 mg/(100 sprouts, and 32.95 mg/(100 sprouts, respectively. This was about 1.11 to 1.40-fold compared to the control culture of 31.40 mg/(100 sprouts. Moreover, the antioxidant capacity of tartary buckwheat sprouts was also enhanced after treatment with the four PS elicitors. Furthermore, the present study revealed the polysaccharide elicitation that caused the accumulation of functional flavonoid by stimulating the phenylpropanoid pathway. The application of beneficial fungal polysaccharide elicitors may be an effective approach to improve the nutritional and functional characteristics of tartary buckwheat sprouts.

  3. Effects of Polysaccharide Elicitors from Endophytic Fusarium oxysporum Fat9 on the Growth, Flavonoid Accumulation and Antioxidant Property of Fagopyrum tataricum Sprout Cultures.

    Science.gov (United States)

    Zhong, Lingyun; Niu, Bei; Tang, Lin; Chen, Fang; Zhao, Gang; Zhao, Jianglin

    2016-11-25

    The purpose of this study was to evaluate the effects of four different fungal polysaccharides, named water-extracted mycelia polysaccharide (WPS), sodium hydroxide-extracted mycelia polysaccharide (SPS), hydrochloric-extracted mycelia polysaccharide (APS), and exo-polysaccharide (EPS) obtained from the endophytic Fusarium oxysporum Fat9 on the sprout growth, flavonoid accumulation, and antioxidant capacity of tartary buckwheat. Without visible changes in the appearance of the sprouts, the exogenous polysaccharide elicitors strongly stimulated sprout growth and flavonoid production, and the stimulation effect was closely related with the polysaccharide (PS) species and its treatment dosage. With application of 200 mg/L of EPS, 200 mg/L of APS, 150 mg/L of WPS, or 100 mg/L of SPS, the total rutin and quercetin yields of buckwheat sprouts were significantly increased to 41.70 mg/(100 sprouts), 41.52 mg/(100 sprouts), 35.88 mg/(100 sprouts), and 32.95 mg/(100 sprouts), respectively. This was about 1.11 to 1.40-fold compared to the control culture of 31.40 mg/(100 sprouts). Moreover, the antioxidant capacity of tartary buckwheat sprouts was also enhanced after treatment with the four PS elicitors. Furthermore, the present study revealed the polysaccharide elicitation that caused the accumulation of functional flavonoid by stimulating the phenylpropanoid pathway. The application of beneficial fungal polysaccharide elicitors may be an effective approach to improve the nutritional and functional characteristics of tartary buckwheat sprouts.

  4. A review of phytochemistry, metabolite changes, and medicinal uses of the common food mung bean and its sprouts (Vigna radiata)

    Science.gov (United States)

    2014-01-01

    The seeds and sprouts of mung bean (Vigna radiata), a common food, contain abundant nutrients with biological activities. This review provides insight into the nutritional value of mung beans and its sprouts, discussing chemical constituents that have been isolated in the past few decades, such as flavonoids, phenolic acids, organic acids, amino acids, carbohydrates, and lipids. Moreover, we also summarize dynamic changes in metabolites during the sprouting process and related biological activities, including antioxidant, antimicrobial, anti-inflammatory, antidiabetic, antihypertensive, lipid metabolism accommodation, antihypertensive, and antitumor effects, etc., with the goal of providing scientific evidence for better application of this commonly used food as a medicine. PMID:24438453

  5. Substituent distribution within cross-linked and hydroxypropylated sweet potato starch and potato starch

    NARCIS (Netherlands)

    Zhao, J.; Schols, H.A.; Chen Zenghong,; Jin Zhengyu,; Buwalda, P.L.; Gruppen, H.

    2012-01-01

    Revealing the substituents distribution within starch can help to understand the changes of starch properties after modification. The distribution of substituents over cross-linked and hydroxypropylated sweet potato starch was investigated and compared with modified potato starch. The starches were

  6. Presence of necrotic strains of Potato virus Y in Mexican potatoes

    Directory of Open Access Journals (Sweden)

    Martínez-Soriano Juan

    2009-06-01

    Full Text Available Abstract Correction to Ramírez-Rodríguez VR, Frías-Treviño G, Aviña-Padilla K, Silva-Rosales L, Martínez-Soria JP: Presence of necrotic strains of Potato virus Y in Mexican potatoes. Virology Journal 2009, 6:48

  7. Comparison of several methods for the extraction of DNA from potatoes and potato-derived products.

    Science.gov (United States)

    Smith, Donna S; Maxwell, Philip W; De Boer, Solke H

    2005-12-28

    Eight methods were compared for the extraction of DNA from raw potato tubers, and nine methods were evaluated for the extraction of DNA from dehydrated potato slices, potato flakes, potato flour, potato starch, and two ready-to-eat potato snack foods. Extracts were assessed for yield using a fluorescence-based DNA quantification assay. Real-time amplification of an endogenous gene, sucrose synthase (sus), was used to assess extract and template quality. A CTAB-based method extracted the highest DNA yields from the tuber material. An in-house method, which utilized the Kingfisher magnetic particle processor, yielded the highest template quality from the tubers. For most of the tuber samples, the Kingfisher and CTAB methods recovered the highest levels of amplifiable sus. DNA yields for potato-derived foods generally decreased with the extent that the product had been processed. The methods that utilized the magnetic particle processor delivered the highest template quality from one of the snack products that was particularly high in fat. For most of the remaining processed products, the levels of amplifiable target DNA recovered were roughly correlated with total DNA recovery, indicating that overall yield had greater influence over sus amplification than template quality. The Wizard method was generally the best method for the extraction of DNA from most of the potato-derived foods.

  8. The use of potato and sweet potato starches affects white salted noodle quality

    NARCIS (Netherlands)

    Chen, Z.; Schols, H.A.; Voragen, A.G.J.

    2003-01-01

    Potato and sweet potato starches and derivatives thereof were used to substitute part of the wheat flour in white salted noodle (WSN) manufacture. The quality of the WSN obtained was compared with the quality of WSN made from wheat flour only. When up to 20% of wheat flour was replaced by acetylated

  9. Immunodetection and quantification of insulin-like antigens in sprouts: development of an efficient functional food

    Directory of Open Access Journals (Sweden)

    Manju Pathak

    2011-11-01

    Full Text Available Background: Hormone Insulin is a drug used for the treatment of type 1 and type 2 Diabetes Mellitus. Insulin used in this experiment is derived from bovine and pork pancreas, as well as through recombinant technology. Patients with diabetes mellitus face an inability to utilize glucose from blood due to either less secretion of insulin, or the inability of the insulin to act; As a result of this glucose levels in the blood rise. The prevention and treatment of type 2 Diabetes Mellitus one is world’s major public health issues. Natural alternatives have a big role to play in this field. This study aims at discovering functional foods rich in Insulin like proteins. Here we are reporting Insulin-like proteins synthesizing during the embryo development stage of Glycine max: soybean, Vigna radiata: moong and Vigna unguiculata: cowpea seeds. Hence, germination transforms these seeds containing human insulin like proteins.Methods: In our investigation we have provided protein extraction with Enzyme-linked immunosorbent assay (ELISA. The plant materials weighing 1g were crushed in mortar and pestle, and the protein from the plant materials was extracted with 20 ml of 0.05 M sodium phosphate buffer (pH 7.6. The suspensions were centrifuged at 6000 rpm for 15 min, and the clear supernatants were subjected to Enzyme linked immunosorbent assay (ELISA for the detection of insulin-like proteins. We have used USDA nutritional data sources for the analysis of new products. Results: Our results demonstrate that Insulin is not expressed in dry mature dormant seeds, but is expressed only during the embryo development stage. Dry mature dormant seeds and the seeds germinated for 24 hours, 48 hours, 72 hours, and 96 hours of Glycine max, Vigna radiata and Vigna unguiculata, were investigated for expression of insulin through immunodetection using anti-insulin antibodies. Dry dormant seeds of all the three seeds showed zero expression at 450 nm for insulin, while

  10. Influence of spatial arrangements in maize/solanum potato ...

    African Journals Online (AJOL)

    Solanum tuberosum L.) on the incidence of potato aphids and leafhoppers was conducted at Namulonge in Uganda during the two growing seasons of 1995. Three potato varieties and one maize variety were intercropped in six spatial ...

  11. 75 FR 14491 - Potato Research and Promotion Plan

    Science.gov (United States)

    2010-03-26

    ...] Potato Research and Promotion Plan AGENCY: Agricultural Marketing Service, USDA. ACTION: Correcting..., Marketing Specialist, Research and Promotion Branch, Fruit and Vegetable Programs, AMS, USDA, 1400..., Marketing agreements, Potatoes, Promotion, Reporting and recordkeeping requirements. 0 Accordingly, 7 CFR...

  12. Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts.

    Science.gov (United States)

    Qian, Hongmei; Liu, Tianyu; Deng, Mingdan; Miao, Huiying; Cai, Congxi; Shen, Wangshu; Wang, Qiaomei

    2016-04-01

    The effects of different light qualities, including white, red and blue lights, on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts were investigated using light-emitting diodes (LEDs) as a light source. Our results showed that blue light treatment significantly decreased the content of gluconapin, the primary compound for bitter flavor in shoots, while increased the glucoraphanin content in roots. Moreover, the maximum content of vitamin C was detected in the white-light grown sprouts and the highest levels of total phenolic and anthocyanins, as well as the strongest antioxidant capacity were observed in blue-light grown sprouts. Taken together, the application of a colorful light source is a good practice for improvement of the consumers' acceptance and the nutritional phtyochemicals of Chinese kale sprouts. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Sprouting productivity and allometric relationships of two oak species managed for traditional charcoal making in central Mexico.

    NARCIS (Netherlands)

    Aguilar, R.; Ghilardi, A.; Vega, E.; Skutsch, Margaret; Oyama, K.

    2012-01-01

    Sustainable production systems for woodfuels in developing countries require basic information on tree productivity, and particularly on their coppicing productivity under current forms of management. We report biomass equations and sprouting productivity of two oak species (Quercus castanea and

  14. WASTE-FREE PRODUCTION TECHNOLOGY OF DRY MASHED POTATOES

    Directory of Open Access Journals (Sweden)

    G. V. Kalashnikov

    2015-01-01

    Full Text Available Summary. According to data on norms of consumption of vegetable production of scientific research institute of Food of the Russian Academy of Medical Science, potatoes win first place with norm of 120 kg a year on the person. In this regard much attention is paid to processing of potatoes that allows to prolong the term of its validity, and also to reduce the capacity of storages and to reduce transport transportations as 1 kg of a dry potatoes produсt is equivalent 7-8 kg of fresh potatoes. Thus industrial processing of potatoes on dry mashed potatoes allows to reduce losses of potatoes at storage and transportation, there is a possibility of enrichment of products vitamins and other useful components, its nutrition value remains better, conditions for complex processing of raw materials with full recycling and creations of stocks of products from potatoes on a crop failure case are created. Dry mashed potatoes are a product of long storage. On the basis of studying of the production technology of mashed potatoes the analysis of technological processes as sources of creation of waste, and the directions of recovery of secondary raw materials for complex waste-free technology of processing of potatoes are defined is provided. The waste-free technological scheme of processing of potatoes and production of dry instant mashed potatoes on the basis of dehydration and moisture thermal treatment a component providing recovery of secondary carbohydrate content raw materials in the form of waste of the main production is developed. The main stages of production of dry instant mashed potatoes are described. It is offered the technological scheme of a production line of mashed potatoes on the basis of waste-free technology. Advantages of the offered waste-free production technology of dry instant mashed potatoes with processing of secondary starch-containing raw materials are given.

  15. Phenolic Compounds in the Potato and Its Byproducts: An Overview

    OpenAIRE

    Akyol, Hazal; Riciputi, Ylenia; Capanoglu, Esra; Caboni, Maria Fiorenza; Verardo, Vito

    2016-01-01

    The potato (Solanum tuberosum L.) is a tuber that is largely used for food and is a source of different bioactive compounds such as starch, dietary fiber, amino acids, minerals, vitamins, and phenolic compounds. Phenolic compounds are synthetized by the potato plant as a protection response from bacteria, fungi, viruses, and insects. Several works showed that these potato compounds exhibited health-promoting effects in humans. However, the use of the potato in the food industry submits this v...

  16. Sweet potato for type 2 diabetes mellitus.

    Science.gov (United States)

    Ooi, Cheow Peng; Loke, Seng Cheong

    2013-09-03

    Sweet potato (Ipomoea batatas) is among the most nutritious subtropical and tropical vegetables. It is also used in traditional medicine practices for type 2 diabetes mellitus. Research in animal and human models suggests a possible role of sweet potato in glycaemic control. To assess the effects of sweet potato for type 2 diabetes mellitus. We searched several electronic databases, including The Cochrane Library (2013, Issue 1), MEDLINE, EMBASE, CINAHL, SIGLE and LILACS (all up to February 2013), combined with handsearches. No language restrictions were used. We included randomised controlled trials (RCTs) that compared sweet potato with a placebo or a comparator intervention, with or without pharmacological or non-pharmacological interventions. Two authors independently selected the trials and extracted the data. We evaluated risk of bias by assessing randomisation, allocation concealment, blinding, completeness of outcome data, selective reporting and other potential sources of bias. Three RCTs met our inclusion criteria: these investigated a total of 140 participants and ranged from six weeks to five months in duration. All three studies were performed by the same trialist. Overall, the risk of bias of these trials was unclear or high. All RCTs compared the effect of sweet potato preparations with placebo on glycaemic control in type 2 diabetes mellitus. There was a statistically significant improvement in glycosylated haemoglobin A1c (HbA1c) at three to five months with 4 g/day sweet potato preparation compared to placebo (mean difference -0.3% (95% confidence interval -0.6 to -0.04); P = 0.02; 122 participants; 2 trials). No serious adverse effects were reported. Diabetic complications and morbidity, death from any cause, health-related quality of life, well-being, functional outcomes and costs were not investigated. There is insufficient evidence about the use of sweet potato for type 2 diabetes mellitus. In addition to improvement in trial methodology

  17. Effect of radiation processing on nutritional and sensory quality of minimally processed green gram and garden pea sprouts

    International Nuclear Information System (INIS)

    Hajare, Sachin N.; Saroj, Sunil D.; Dhokane, Varsha S.; Shashidhar, R.; Bandekar, Jayant R.

    2007-01-01

    In the present study, radiation processing of minimally processed green gram and garden pea sprouts was carried out at doses 1 and 2 kGy. The effect of this treatment on different quality parameters like vitamin C content, total carotenoids content, sensory quality, texture, and color was determined over a storage period of 12 days at two different temperatures, a 4 and 8 deg. C. It was observed that treatment of irradiation (1 and 2 kGy) and storage period did not have any significant effect on vitamin C content of control as well as irradiated sprout samples stored at 4 and 8 deg.C. Total carotenoids content of sprouts stored at 4, as well as at 8 deg. C, for 12 days remained almost unchanged after irradiation as well as during storage. Sensory evaluation studies showed that irradiation had no significant effect (p>0.05) on the ratings of any of the sensory attributes in green gram as well as garden pea sprouts and, thus, did not alter the overall acceptability of the irradiated sprouts. Textural studies revealed that there was no significant change (p>0.05) in the firmness of irradiated sprouts (1 and 2 kGy) as compared to control samples at both the temperatures. Storage period of 12 days also did not affect the firmness of sprouts significantly. Color measurement results indicated no drastic change in the color coordinates of the green gram samples except greenness of controls stored at both the temperatures, which showed insignificant decrease in the a * values. Thus, the nutritional as well as sensory quality of minimally processed green gram and garden pea sprouts did not alter significantly after gamma irradiation with a dose of 1 and 2 kGy

  18. Memristors: Memory elements in potato tubers.

    Science.gov (United States)

    Volkov, Alexander G; Nyasani, Eunice K; Blockmon, Avery L; Volkova, Maya I

    2015-01-01

    A memristor is a nonlinear element because its current-voltage characteristic is similar to that of a Lissajous pattern for nonlinear systems. This element was postulated recently and researchers are looking for it in different biosystems. We investigated electrical circuitry of red Irish potato tubers (Solanum tuberosum L.). The goal was to discover if potato tubers might have a new electrical component - a resistor with memory. The analysis was based on a cyclic current-voltage characteristic where the resistor with memory should manifest itself. We found that the electrostimulation by bipolar sinusoidal or triangle periodic waves induces electrical responses in the potato tubers with fingerprints of memristors. Tetraethylammonium chloride, an inhibitor of voltage gated K(+) channels, transforms a memristor to a resistor in potato tubers. Our results demonstrate that a voltage gated K(+) channel in the excitable tissue of potato tubers has properties of a memristor. Uncoupler carbonylcyanide-4-trifluoromethoxy-phenyl hydrazone decreases the amplitude of electrical responses at low and high frequencies of bipolar periodic sinusoidal or triangle electrostimulating waves. The discovery of memristors in plants creates a new direction in the understanding of electrical phenomena in plants.

  19. Evaluation of five pre-emergence herbicides for volunteer potato ...

    African Journals Online (AJOL)

    Volunteer potatoes can cause significant weed problems in crops following potatoes as large numbers of potato tubers remain behind in the field after mechanical harvesting. These volunteer plants can create havoc with rotation programs and serve as a source of pests and diseases. The aim of this project was to identify a ...

  20. Proximate analysis of Sweet Potato Toasted Granules | Meludu ...

    African Journals Online (AJOL)

    Sweet potato is an important root crop in the food system of many African countries. The yield, nutrition and economic potential of sweet potato have been identified as very high. In this study, sweet potato was processed and toasted into granules. The proximate analysis performed on the toasted granules showed protein, ...

  1. Determinants of sweet potato value addition among smallholder ...

    African Journals Online (AJOL)

    Sweet potato is an important food security promoted crop in Nigeria. The recognition of its relative health benefits has resulted in fresh consumption as well as the utilization of processed products such as sweet potato chips, fries and pre-cut, flour, and pureed sweet potatoes. This study examined the determinants of sweet ...

  2. Molecular and genetic analyses of potato cyst nematode resistance loci

    NARCIS (Netherlands)

    Bakker, E.H.

    2003-01-01

    This thesis describes the genomic localisation and organisation of loci that harbour resistance to the potato cyst nematode species Globodera pallida and G. rostochiensis . Resistance to the potato cyst nematodes G. pallida and G. rostochiensis is an important aspect in potato breeding. To gain

  3. Genetic Variability in Potato (Solanum tuberosum L.) Genotypes for ...

    African Journals Online (AJOL)

    Late blight (Phytophthora infestans de Bary) is the most important and destructive disease of potato (Solanum tuberosum L). The pathogen has the ability to rapidly evolve and overcome resistance genes, leading commercial potato varieties to succumb to it too soon. As a result, evaluation of commercial potato varieties for ...

  4. Influence of Treatment of Seed Potato Tubers with Plant Crude ...

    African Journals Online (AJOL)

    A pot experiment was conducted at Sirinka Agricultural Research Centre, in north eastern Ethiopia, to evaluate the effect of treating seed potato tubers with crude plant essential oil extracts, on the growth and yield of the potato crop. Treatments consisted of seed potato tubers treated with dill weed, spearmint, black cumin ...

  5. Least-Cost Seed Potato Production in Ethiopia

    NARCIS (Netherlands)

    Tufa, A.H.; Gielen-Meuwissen, M.P.M.; Lommen, W.J.M.; Tsegaye, A.; Struik, P.C.; Oude Lansink, A.G.J.M.

    2015-01-01

    Improved potato varieties can increase potato yields of smallholders, and thus contribute to food security improvement in Ethiopia. However, the uptake of these varieties by farmers is very limited so far and this is one of the causes of insufficient seed quality in the seed potato system in

  6. Challenges and opportunities for quality seed potato availability and ...

    African Journals Online (AJOL)

    Potato yields in Uganda have remained low at 7.5 t ha-1. This low yield is attributed to low yielding varieties, poor management practices and lack of quality seed potatoes among other factors. This study was conducted to ascertain the challenges and opportunities of quality seed potato availability and production in the ...

  7. Tuber formation in the wild potato species Solanum demissum Lindl.

    NARCIS (Netherlands)

    Helder, J.

    1994-01-01

    1. How does a potato plant form tubers?

    Potato plants produce sexual multiplication and survival structures, true seeds, and asexual multiplication and survival bodies, tubers. Berries of the potato plant contain a large number of minute seeds. Relatively large

  8. Cell cycle and storage related gene expression in potato tubers

    NARCIS (Netherlands)

    Verhees, J.

    2002-01-01

    Potato tubers are vegetative structures aimed at survival of the period when conditions are not optimal for the growth of the potato plant. Understanding the process of tuber formation in potatoes ( Solanum tuberosum ) is important, both from a scientific view, and for

  9. Glycoalkaloids and phenolic compounds in gamma irradiated potatoes

    International Nuclear Information System (INIS)

    Bergers, W.W.A.

    1980-01-01

    Potatoes were used to study the metabolic stress effects in irradiated vegetable products. The changes of the contents of specific target compounds (glycoalkaloids, phenolic acids and coumarins) in alcoholic extracts of gamma irradiated potatoes were studied for metabolic irradiation stress. Doses of up to 3 kGy were applied to potatoes of several varieties. (Auth.)

  10. Taxonomy of Cultivated Potatoes (Solanum section Petota: Solanaceae)

    Science.gov (United States)

    Solanum tuberosum, the cultivated potato of world commerce, is a primary food crop worldwide. Wild and cultivated potatoes form the germplasm base for international breeding efforts to improve potato in the face of variety of disease, environmental, and agronomic constraints. A series of national an...

  11. Increasing genetic gain by reducing ploidy in potato

    Science.gov (United States)

    While potato cultivars in major world production regions are tetraploid, wild and cultivated potatoes in the crop’s center of origin range from diploid to hexaploid. Landrace potato varieties cannot be distinguished based on ploidy. Contrary to popular belief, tetraploidy does not appear to be neces...

  12. Methods for the preparation of cell walls from potatoes

    NARCIS (Netherlands)

    Jardine, W.G.; Doeswijk-Voragen, C.H.L.; MacKinnon, I.M.R.; Broek, van den L.A.M.; Ha, M.A.; Jarvis, C.; Voragen, A.G.J.

    2002-01-01

    A group of new methods is described for preparing cell walls from potatoes and processed potato products. Starting from raw domestic potatoes, starch is degraded enzymatically after a very brief 100 °C gelatinisation step conducted after homogenisation to minimise the time required for heat

  13. Native protein recovery from potato fruit juice by ultrafiltration

    NARCIS (Netherlands)

    Zwijnenberg, Harmen Jan; Kemperman, Antonius J.B.; Boerrigter, M.E.; Lotz, Martin; Dijksterhuis, Jan F.; Koops, G.H.; Poulsen, Poul Emil

    2002-01-01

    Potato fruit juice, i.e. the stream resulting after the extraction of the starch from the potato, contains up to 2.5% [w/w] of proteins that are potentially valuable for the food market. However, today the recovery of protein from the potato fruit juice with reverse osmosis membranes results in a

  14. Pinpointing the weed sources of potato psyllid in Washington

    Science.gov (United States)

    A major challenge in controlling zebra chip disease of potatoes is our inability to predict what potato fields are likely to be colonized by potato psyllid, the vector of the pathogen that causes zebra chip. Researchers at the USDA-ARS laboratory in Wapato, WA developed a novel method to identify w...

  15. Effects of a Brussels sprouts extract on oxidative DNA damage and metabolising enzymes in rat liver

    DEFF Research Database (Denmark)

    Sørensen, Mette; Jensen, B.R.; Poulsen, Henrik E.

    2001-01-01

    in the liver. Oral administration of an aqueous Brussels sprouts extract for 4 days was found to induce the expression of GST 1.3-fold (P antioxidant......The apparent anticarcinogenic effect of cruciferous vegetables found in numerous epidemiological and experimental studies has been associated with their influence on phase I and phase II metabolising enzymes as well as on the antioxidant status. In the present study we investigated the effect...... of administration of a Brussels sprouts extract on the expression at the mRNA level and/or catalytic activity in rat liver of three phase I enzymes [cytochrome P450-1A2 (CYP1A2),-2B1/2 (CYP2B1/2) and-2E1 (CYP2E1)] and two phase II enzyme [NADPH:quinone reductase (QR) and glutathione S-transferase pi 7 (GSTpi)], all...

  16. Starch and protein analysis of wheat bread enriched with phenolics-rich sprouted wheat flour.

    Science.gov (United States)

    Świeca, Michał; Dziki, Dariusz; Gawlik-Dziki, Urszula

    2017-08-01

    Wheat flour in the bread formula was replaced with sprouted wheat flour (SF) characterized by enhanced nutraceutical properties, at 5%, 10%, 15% and 20% levels. The addition of SF slightly increased the total protein content; however, it decreased their digestibility. Some qualitative and quantitative changes in the electrophoretic pattern of proteins were also observed; especially, in the bands corresponding with 27kDa and 15-17kDa proteins. These results were also confirmed by SE-HPLC technique, where a significant increase in the content of proteins and peptides (molecular masses breads with 20% of SF. Bread enriched with sprouted wheat flour had more resistant starch, but less total starch, compared to control bread. The highest in vitro starch digestibility was determined for the control bread. The studied bread with lowered nutritional value but increased nutritional quality can be used for special groups of consumers (obese, diabetic). Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Promotion of neural sprouting using low-level green light-emitting diode phototherapy

    Science.gov (United States)

    Alon, Noa; Duadi, Hamootal; Cohen, Ortal; Samet, Tamar; Zilony, Neta; Schori, Hadas; Shefi, Orit; Zalevsky, Zeev

    2015-02-01

    We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100 mW/cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.

  18. Axonal sprouting regulates myelin basic protein gene expression in denervated mouse hippocampus

    DEFF Research Database (Denmark)

    Jensen, M B; Poulsen, F R; Finsen, B

    2000-01-01

    to 35 days after transection of the entorhino-hippocampal perforant path axonal projection. In situ hybridization analysis showed that anterograde axonal and terminal degeneration lead to upregulated oligodendrocyte MBP mRNA expression starting between day 2 and day 4, in (1) the deep part of stratum...... axonal and terminal degeneration, myelin degenerative changes, microglial activation and axotomi-induced axonal sprouting. Oligodendrocyte MBP mRNA expression reached maximum in both these areas at day 7. MBP gene transcription remained constant in stratum radiatum, stratum pyramidale and stratum oriens...... of CA1, areas that were unaffected by perforant path transection. These results provide strong evidence that oligodendrocyte MBP gene expression can be regulated by axonal sprouting independently of microglial activation in the injured adult CNS....

  19. Melatonin Attenuates Potato Late Blight by Disrupting Cell Growth, Stress Tolerance, Fungicide Susceptibility and Homeostasis of Gene Expression in Phytophthora infestans

    Directory of Open Access Journals (Sweden)

    Shumin Zhang

    2017-11-01

    Full Text Available Phytophthora infestans (P. infestans is the causal agent of potato late blight, which caused the devastating Irish Potato Famine during 1845-1852. Until now, potato late blight is still the most serious threat to potato growth and has caused significant economic losses worldwide. Melatonin can induce plant innate immunity against pathogen infection, but the direct effects of melatonin on plant pathogens are poorly understood. In this study, we investigated the direct effects of melatonin on P. infestans. Exogenous melatonin significantly attenuated the potato late blight by inhibiting mycelial growth, changing cell ultrastructure, and reducing stress tolerance of P. infestans. Notably, synergistic anti-fungal effects of melatonin with fungicides on P. infestans suggest that melatonin could reduce the dose levels and enhance the efficacy of fungicide against potato late blight. A transcriptome analysis was carried out to mine downstream genes whose expression levels were affected by melatonin. The analysis of the transcriptome suggests that 66 differentially expressed genes involved in amino acid metabolic processes were significantly affected by melatonin. Moreover, the differentially expressed genes associated with stress tolerance, fungicide resistance, and virulence were also affected. These findings contribute to a new understanding of the direct functions of the melatonin on P. infestans and provide a potential ecofriendly biocontrol approach using a melatonin-based paradigm and application to prevent potato late blight.

  20. Embryonic stem cell-derived embryoid bodies development in collagen gels recapitulates sprouting angiogenesis.

    Science.gov (United States)

    Feraud, O; Cao, Y; Vittet, D

    2001-12-01

    The formation of new blood vessels proceeds by both vasculogenesis and angiogenesis. The development of models, which fully recapitulate spatio-temporal events involved during these processes, are crucial to fully understand their mechanisms of regulation. In vitro differentiation of murine embryonic stem (ES) cells has been shown to be a useful tool to investigate factors and genes potentially involved in vasculogenesis (Hirashima et al, 1999; Risau et al, 1988; Vittet et al, 1996; Wang et al, 1992; Wartenberg et al, 1998). We asked here whether this model system can also recapitulate angiogenesis, which may offer new means to study mechanisms involved in this process. ES-derived embryoid bodies (EBs) obtained after 11 days of differentiation, in which a primitive vascular network had formed, were then subcultured into a type I collagen matrix. In the presence of angiogenic growth factors, EBs rapidly developed branching pseudopods. Whole mount immunostainings with a PECAM antibody revealed that more than 75% EBs displayed, within a few days, a large number of endothelial outgrowths that can give tube-like structures with concomitant differentiation of alpha-smooth muscle actin positive cells, thus evoking sprouting angiogenesis. High expression levels of flk1 (VEGFR2), flt1 (VEGFR1), tie-1, and tie-2 are also found, indicating that budding endothelial cells displayed an angiogenic phenotype. The endothelial sprouting response was specifically induced by angiogenic factors with a major contribution of vascular endothelial growth factor (VEGF). Known angiostatic agents, such as platelet factor 4 (PF4), angiostatin, and endostatin inhibited the formation of endothelial sprouts induced by angiogenic factors. Moreover, consistent with the in vivo phenotype, VE-cadherin deficient EBs failed to develop angiogenesis in this model. ES cell differentiation can then recapitulate, in addition to vasculogenesis, the early stages of sprouting angiogenesis. This model system

  1. TNF primes endothelial cells for angiogenic sprouting by inducing a tip cell phenotype

    OpenAIRE

    Sainson, Richard C. A.; Johnston, Douglas A.; Chu, Henry C.; Holderfield, Matthew T.; Nakatsu, Martin N.; Crampton, Steven P.; Davis, Jaeger; Conn, Erin; Hughes, Christopher C. W.

    2008-01-01

    Pathological angiogenesis associated with wound healing often occurs subsequent to an inflammatory response that includes the secretion of cytokines such as tumor necrosis factor (TNF). Controversy exists on the angiogenic actions of TNF, with it being generally proangiogenic in vivo, but antiangiogenic in vitro. We find that whereas continuous administration of TNF in vitro or in vivo inhibits angiogenic sprouting, a 2- to 3-day pulse stimulates angiogenesis by inducing an endothelial “tip c...

  2. Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens

    Science.gov (United States)

    Fahey, Jed W.; Zhang, Yuesheng; Talalay, Paul

    1997-01-01

    Induction of phase 2 detoxication enzymes [e.g., glutathione transferases, epoxide hydrolase, NAD(P)H: quinone reductase, and glucuronosyltransferases] is a powerful strategy for achieving protection against carcinogenesis, mutagenesis, and other forms of toxicity of electrophiles and reactive forms of oxygen. Since consumption of large quantities of fruit and vegetables is associated with a striking reduction in the risk of developing a variety of malignancies, it is of interest that a number of edible plants contain substantial quantities of compounds that regulate mammalian enzymes of xenobiotic metabolism. Thus, edible plants belonging to the family Cruciferae and genus Brassica (e.g., broccoli and cauliflower) contain substantial quantities of isothiocyanates (mostly in the form of their glucosinolate precursors) some of which (e.g., sulforaphane or 4-methylsulfinylbutyl isothiocyanate) are very potent inducers of phase 2 enzymes. Unexpectedly, 3-day-old sprouts of cultivars of certain crucifers including broccoli and cauliflower contain 10–100 times higher levels of glucoraphanin (the glucosinolate of sulforaphane) than do the corresponding mature plants. Glucosinolates and isothiocyanates can be efficiently extracted from plants, without hydrolysis of glucosinolates by myrosinase, by homogenization in a mixture of equal volumes of dimethyl sulfoxide, dimethylformamide, and acetonitrile at −50°C. Extracts of 3-day-old broccoli sprouts (containing either glucoraphanin or sulforaphane as the principal enzyme inducer) were highly effective in reducing the incidence, multiplicity, and rate of development of mammary tumors in dimethylbenz(a)anthracene-treated rats. Notably, sprouts of many broccoli cultivars contain negligible quantities of indole glucosinolates, which predominate in the mature vegetable and may give rise to degradation products (e.g., indole-3-carbinol) that can enhance tumorigenesis. Hence, small quantities of crucifer sprouts may protect

  3. Disturbance is an important factor in the evolution and distribution of root-sprouting species

    Czech Academy of Sciences Publication Activity Database

    Klimešová, Jitka; Herben, Tomáš; Martínková, Jana

    2017-01-01

    Roč. 31, č. 3 (2017), s. 387-399 ISSN 0269-7653 R&D Projects: GA ČR(CZ) GA16-19245S; GA ČR GB14-36079G Institutional support: RVO:67985939 Keywords : disturbance * root-sprouting species * bud bank Subject RIV: EH - Ecology, Behaviour OBOR OECD: Ecology Impact factor: 1.818, year: 2016

  4. The Effects of Light and Temperature on Biotin Synthesis in Pea Sprouts.

    Science.gov (United States)

    Kamiyama, Shin; Ohnuki, Risa; Moriki, Aoi; Abe, Megumi; Ishiguro, Mariko; Sone, Hideyuki

    2016-01-01

    Biotin is an essential micronutrient, and is a cofactor for several carboxylases that are involved in the metabolism of glucose, fatty acids, and amino acids. Because plant cells can synthesize their own biotin, a wide variety of plant-based foods contains significant amounts of biotin; however, the influence of environmental conditions on the biotin content in plants remains largely unclear. In the present study, we investigated the effects of different cultivation conditions on the biotin content and biotin synthesis in pea sprouts (Pisum sativum). In the experiment, the pea sprouts were removed from their cotyledons and cultivated by hydroponics under five different lighting and temperature conditions (control [25ºC, 12-h light/12-h dark cycle], low light [25ºC, 4-h light/20-h dark cycle], dark [25ºC, 24 h dark], low temperature [12ºC, 12-h light/12-h dark cycle], and cold [6ºC, 12-h light/12-h dark cycle]) for 10 d. Compared to the biotin content of pea sprouts under the control conditions, the biotin contents of pea sprouts under the low-light, dark, and cold conditions had significantly decreased. The dark group showed the lowest biotin content among the groups. Expression of the biotin synthase gene (bio2) was also significantly decreased under the dark and cold conditions compared to the control condition, in a manner similar to that observed for the biotin content. No significant differences in the adenosine triphosphate content were observed among the groups. These results indicate that environmental conditions such as light and temperature modulate the biotin content of pea plant tissues by regulating the expression of biotin synthase.

  5. High microbial load in sprouts and ready-to-eat salad mixtures

    OpenAIRE

    German Federal Institute for Risk Assessment

    2010-01-01

    Fresh sprouts and ready-to-eat salad mixtures sold in bags which were cut, washed and packaged days earlier are considered as perishable products. Though they are stored under refrigerated conditions, there is a danger of microbial deterioration and contamination with bacteria such as Listeria, Salmonella, E. coli bacteria or viruses such as norovirus or hepatitis A virus. These bacteria can cause a range of foodborne infections of varying severity with symptoms such as nausea, indigestion, b...

  6. Radiation induced variation in potato for tolerance to salinity using tissue culture technique

    International Nuclear Information System (INIS)

    Sharabash, M.T.

    2001-01-01

    Meristem-tips of potato (Solanum tuberosum) cv. 'Diamant', obtained from tuber sprouts, were cultured on MS medium, and multiplied into plantlets through micropropagation. To induce variation for salt tolerance, the obtained plantlets were irradiated with 0, 20, and 40 Gy gamma rays at 27.7 rad/sec. Irradiated plantlets were cut into single nodes and cultured on MS medium, supplemented with 2000 and 4000 ppm NaCI. Salt tolerant plantlets were transferred for tuberization on MS liquid medium supplemented with the same concentration of NaCI. Micro-tubers, collected after 6 weeks of culture, had fresh weight between 0.03 to 0.3 g. Mini-tubers were obtained by planting micro-tubers in 25 cm pots under insect proof greenhouse. Mini-tuber number per plant ranged from 3 to 6, and the mini-tuber weight ranged from 0.5-3.0 g, depending upon the treatment. Further studies are in progress to produce conventional tubers under salinity stress from the promising variants, specially those tolerant to 4000 ppm, and to assure the stability of the obtained variants. (author)

  7. Salt Tolerant Mutants in Potato sp. Cara Induced by Gamma Irradiation and Tissue Culture Technique

    International Nuclear Information System (INIS)

    Sharabash, M.T.; Mohamed, A.A.; Ahmed, F.A.; Afifi, A.M.

    2003-01-01

    Sprouts of 2-3 cm length were cut off from potatoes cv. Cara tubers and sterilized. Apical meristemic tips were sown to produce virus free plantlets. After 6-8 weeks, the new plantlets became at 5-7cm heights. Micro-propagation was begun to obtain sufficient number of plantlets. Plantlets obtained from micro-propagation stage were divided into 3 groups to be exposed to 0.0,20 or 40Gy gamma rays. The dose rate was 27.7rad/sec. Irradiated and unirradiated plantlets were trimmed to be with 5-6 nodes and without leaves. They were transplanted onto 1/2MS [1] liquid medium supplemented with sodium chloride at different concentrations, i.e.,0.0, 2000 or 4000 ppm. Healthy plantlets were chosen, and all unfit ones were excluded. The plantlets were sub-cultured six times on the same fresh medium with the same concentrations of NaCI, with insisting to exclude all unfit plantlets. All maintained healthy plantlets, in the sixth vegetative generation (M 1 V 6 ), grown under salinity stress, whatever the concentration of NaCI, were considered as mutant lines

  8. Physico-chemical and sensory evaluation of potato (Solanum tuberosum L.) after irradiation.

    Science.gov (United States)

    Soares, Ivanesa G M; Silva, Edvane B; Amaral, Ademir J; Machado, Erilane C L; Silva, Josenilda M

    2016-06-07

    This work evaluated the effects of ionizing radiation on the physico-chemical and sensory characteristics of the potato cultivar Ágata (Solanum tuberosum L.), including budding and deterioration, with the end goal of increasing shelf life. For this, four groups of samples were harvested at the maturation stage. Three of them were separately exposed to a Co-60 source, receiving respective doses of 0.10, 0.15 and 2.00 kGy, while the non-irradiated group was kept as a control. All samples were stored for 35 days at 24 °C (± 2) and at 39% relative humidity. The following aspects were evaluated: budding, rot, loss of weight, texture, flesh color, moisture, external and internal appearance, aroma, soluble solids, titratable acidity, vitamin C, protein, starch and glucose. The results indicated that 0.15 kGy was the most effective dose to reduce sprouting and post-harvest losses, under the conditions studied.

  9. Physico-chemical and sensory evaluation of potato (Solanum tuberosum L. after irradiation

    Directory of Open Access Journals (Sweden)

    IVANESA G.M. SOARES

    2016-06-01

    Full Text Available ABSTRACT This work evaluated the effects of ionizing radiation on the physico-chemical and sensory characteristics of the potato cultivar Ágata (Solanum tuberosum L., including budding and deterioration, with the end goal of increasing shelf life. For this, four groups of samples were harvested at the maturation stage. Three of them were separately exposed to a Co-60 source, receiving respective doses of 0.10, 0.15 and 2.00 kGy, while the non-irradiated group was kept as a control. All samples were stored for 35 days at 24 °C (± 2 and at 39% relative humidity. The following aspects were evaluated: budding, rot, loss of weight, texture, flesh color, moisture, external and internal appearance, aroma, soluble solids, titratable acidity, vitamin C, protein, starch and glucose. The results indicated that 0.15 kGy was the most effective dose to reduce sprouting and post-harvest losses, under the conditions studied.

  10. Effect of gamma irradiation and CIPC treatment- on processing quality of potatoes

    International Nuclear Information System (INIS)

    Shirsat, S.G.; Thomas, Paul; Nair, P.M.

    1994-01-01

    The processing quality of 5 potato cultivars, after inhibition of sprouting by irradiation or chloroisopropyl phenyl carbamate (CIPC) treatment, was compared during storage at 20, 15 and 10 degC upto 9 months. Cultivars K. Jyoti, K Lavkar, Gujrat-S, and Talegaon, with initial reducing sugar levels of 120 to 240 mg/100 g. yielded light coloured chips and French fries. Neither of the treatments affected the processing quality of these cultivars, when stored at 15 and 20 degC up to 6 months, but the storage at 10 degC resulted in darker products, due to higher levels of reducing sugars. In tubers, stored for more than 6 months at 15 degC, reconditioning at 27-32 degC or blanching improved the colour of the products. Cultivars K. Badshah, with initial reducing sugar levels of 310 to 440 mg/100 g was not suitable for processing. In vitro infiltration studies showed that glucose contributed to browning more than fructose or sucrose. (author). 17 refs., 5 figs., 1 tab

  11. In vitro induction of variation through radiation for late blight resistance and heat tolerance in potato

    International Nuclear Information System (INIS)

    Minocha, J.L.; Das, A.; Gopal, J.; Gosal, S.S.

    1997-01-01

    In vitro plants were obtained from nodal sections of sprouts of cvs. 'Kufri Jyoti' and 'Kufri Chandramukhi' of potato cultured on MS medium with 3% sucrose. Callus from leaves of in vitro cultured plantlets was induced on modified Linsmaier and Skoog medium supplemented with 5 mg/1 NAA. The obtained shoots and calli were irradiated with 20 and 40 Gy gamma rays. Irradiatied shoots were transferred to MS medium with 8% sucrose for multiplication, and then to MS medium with 8% sucrose and 10 mg/1 BAP to induce microtuber formation, which gave on average 1.3 microtubers per plant. The microtubers were planted in pots and variation was observed in plant morphology and tuber characters. To study variation for late blight resistance, irradiated calli were kept on Gamborg B-5 medium with culture filtrate of Phytophthora infestans. To induce variation for heat tolerance, in vitro shoots from irradiated material were mass-propagated and allowed to produce microtubers at high temperature. (author). 3 refs, 3 tabs

  12. Redundant dopaminergic activity may enable compensatory axonal sprouting in Parkinson disease.

    Science.gov (United States)

    Arkadir, David; Bergman, Hagai; Fahn, Stanley

    2014-03-25

    Neurodegenerative diseases become clinically apparent only after a substantial population of neurons is lost. This raises the possibility of compensatory mechanisms in the early phase of these diseases. The importance of understanding these mechanisms cannot be underestimated because it may guide future disease-modifying strategies. Because the anatomy and physiology of the nigrostriatal dopaminergic pathways have been well described, the study of Parkinson disease can offer insight into these early compensatory mechanisms. Collateral axonal sprouting of dopaminergic terminals into the denervated striatum is the most studied compensatory mechanism in animal (almost exclusively rodent) models of Parkinson disease and is correlated with behavioral recovery after partial lesions. This sprouting, however, does not respect the normal anatomy of the original nigrostriatal pathways and leads to aberrant neuronal networks. We suggest here that the unique physiologic property of the dopaminergic innervation of the striatum, namely redundancy of information encoding, is crucial to the efficacy of compensatory axonal sprouting in the presence of aberrant anatomical connections. Redundant information encoding results from the similarity of representation of salient and rewarding events by many dopaminergic neurons, from the wide axonal field of a single dopaminergic neuron in the striatum, and from the nonspecific spatial effect of dopamine on striatal neurons (volume conductance). Finally, we discuss the relevance of these findings in animal models to human patients with Parkinson disease.

  13. Hardwood re-sprout control in hydrologically restored Carolina Bay depression wetlands.

    Energy Technology Data Exchange (ETDEWEB)

    Moser, Lee, Justin

    2009-06-01

    Carolina bays are isolated depression wetlands located in the upper coastal plain region of the eastern Unites States. Disturbance of this wetland type has been widespread, and many sites contain one or more drainage ditches as a result of agricultural conversion. Restoration of bays is of interest because they are important habitats for rare flora and fauna species. Previous bay restoration projects have identified woody competitors in the seedbank and re-sprouting as impediments to the establishment of herbaceous wetland vegetation communities. Three bays were hydrologically restored on the Savannah River Site, SC, by plugging drainage ditches. Residual pine/hardwood stands within the bays were harvested and the vegetative response of the seedbank to the hydrologic change was monitored. A foliar herbicide approved for use in wetlands (Habitat® (Isopropylamine salt of Imazapyr)) was applied on one-half of each bay to control red maple (Acer rubrum L.), sweet gum (Liquidambar styraciflua L.), and water oak (Quercus nigra L.) sprouting. The effectiveness of the foliar herbicide was tested across a hydrologic gradient in an effort to better understand the relationship between depth and duration of flooding, the intensity of hardwood re-sprout pressure, and the need for hardwood management practices such as herbicide application.

  14. Effect of various solvent on the specific amino acids of black soybean (Glycine soja) sprout

    Science.gov (United States)

    Kanetro, B.; Slamet, A.; Wazyka, A.

    2018-01-01

    The objective of this research was to study the effect of various solvent extractions on the specific amino acids as small peptide or free amino acids that was contained in the extract after removal of the macromolecule protein of black soybean sprouts. The experimental design of this research was randomized complete design with one factor, which was the three various solvent, i.e. hexane, ethanol and water. The black soybean seed was germinated for 36 h. The small peptide and free amino acids of black soybean sprout were isolated at 3 various of solvents extraction, and then the macromolecule proteins in the extracts were precipitated at the pH 4. The extracts of black soybean sprout after removal of the macromolecule protein were analysed by HPLC to determine the profile of amino acids for stimulation of insulin secretion. The result of this research showed that the extracts contained the small peptide and free amino acid for stimulation of insulin secretion. The best solvent extraction was water that was due to the content of Leu, Arg, Ala, Phe, Ile, and Lys of water extract was higher than hexane and ethanol extracts.

  15. Typicity in Potato: Characterization of Geographic Origin

    Directory of Open Access Journals (Sweden)

    Marco Manzelli

    2010-03-01

    Full Text Available A two-year study was carried out in three regions of Italy and the crop performance and the chemical composition of tubers of three typical potato varieties evaluated. Carbon and nitrogen tuber content was determined by means of an elemental analyzer and the other mineral elements by means of a spectrometer. The same determinations were performed on soil samples taken from experimental areas. The Principal Component Analysis, applied to the results of mineral element tuber analysis, permitted the classification of all potato tuber samples according to their geographic origin. Only a partial discrimination was obtained in function of potato varieties. Some correlations between mineral content in the tubers and in the soil were also detected. Analytical and statistical methods proved to be useful in verifying the authenticity of guaranteed geographical food denominations.

  16. Acrylamide Mitigation Procedures in Fried Potatoes

    DEFF Research Database (Denmark)

    Pedreschi, Franco; Kaack, Karl; Granby, Kit

    2008-01-01

    , potato slices were treated in one of the following ways: (i) blanched in hot water at six different time-temperature combinations (50 degrees C for 30 and 70 min; 70 degrees C for 8 and 40 min; 90 degrees C for 2 and 9 min); (ii) immersed in a citric acid solutions of 10 g/L for half an hour Potato...... surprisingly had a very low acrylamide content (28 mu m/kg) even when they were fried at 190 degrees C. Potato immersion in citric acid solution of 10 g/L reduced acrylamide formation by almost 70% for slices fried at 150 degrees C. Color represented by the parameters L* and a* showed high correlations (r(2...

  17. COSIANA – NEW ROMANIAN POTATO VARIETY

    Directory of Open Access Journals (Sweden)

    Radu Hermeziu

    2016-07-01

    Full Text Available Potato breeding are oriented to obtain varieties with resistance to biotic and abiotic factors and with high yield capacity to satisfy the both quantitative and qualitative needs of consumers. Productivity, quality and stability are achieved through crop improvement works to promote new varieties with traits performance. Among the achievements of National Institute of Research and Development for Potato and Sugar Beet in 2015 was registred the variety Cosiana. The variety is obtained through sexual hybridization and individual clonal selection. As vegetation period enroll in the group of middle varieties. Cosiana has a high yield capacity, is resistant to black wart (Synchitrium endobioticum, middle resistant to late blight (Phytopththora infestans and to different viruses (PVY0 and PLRV. The variety is conceived for autumn-winter consumption, being suitable for most culinary preparations, from salad to mash potatoes.

  18. In vivo wound healing and antiulcer properties of white sweet potato (Ipomoea batatas).

    Science.gov (United States)

    Hermes, Daniele; Dudek, Débora N; Maria, Mariana D; Horta, Lívia P; Lima, Eliete N; de Fátima, Ângelo; Sanches, Andréia C C; Modolo, Luzia V

    2013-07-01

    The potential of tuber flour of Ipomoea batatas (L.) Lam. cv. Brazlândia Branca (white sweet potato) as wound healing and antiulcerogenic agent was investigated in vivo in animal model. Excision on the back of Wistar rats was performed to induce wounds that were topically treated with Beeler's base containing tuber flour of white sweet potato at 2.5%. Number of cells undergoing metaphase and the degree of tissue re-epithelialization were investigated 4, 7 and 10 days post-treatment. The protective effect of aqueous suspension of tuber flour (75 and 100 mg/kg animal weight) on gastric mucosa of Wistar rats was also studied by using the ethanol-induced ulceration model. Ointment based on white sweet potato at 2.5% effectively triggered the healing of cutaneous wound as attested by the increased number of cells undergoing metaphase and tissue re-epithelialization regardless the time of wound treatment. Tuber flour potentially prevented ethanol-induced gastric ulceration by suppressing edema formation and partly protecting gastric mucosa wrinkles. Crude extracts also exhibited potential as free radical scavengers. The results from animal model experiments indicate the potential of tuber flour of white sweet potato to heal wounds.

  19. In vivo wound healing and antiulcer properties of white sweet potato (Ipomoea batatas)

    Science.gov (United States)

    Hermes, Daniele; Dudek, Débora N.; Maria, Mariana D.; Horta, Lívia P.; Lima, Eliete N.; de Fátima, Ângelo; Sanches, Andréia C.C.; Modolo, Luzia V.

    2012-01-01

    The potential of tuber flour of Ipomoea batatas (L.) Lam. cv. Brazlândia Branca (white sweet potato) as wound healing and antiulcerogenic agent was investigated in vivo in animal model. Excision on the back of Wistar rats was performed to induce wounds that were topically treated with Beeler’s base containing tuber flour of white sweet potato at 2.5%. Number of cells undergoing metaphase and the degree of tissue re-epithelialization were investigated 4, 7 and 10 days post-treatment. The protective effect of aqueous suspension of tuber flour (75 and 100 mg/kg animal weight) on gastric mucosa of Wistar rats was also studied by using the ethanol-induced ulceration model. Ointment based on white sweet potato at 2.5% effectively triggered the healing of cutaneous wound as attested by the increased number of cells undergoing metaphase and tissue re-epithelialization regardless the time of wound treatment. Tuber flour potentially prevented ethanol-induced gastric ulceration by suppressing edema formation and partly protecting gastric mucosa wrinkles. Crude extracts also exhibited potential as free radical scavengers. The results from animal model experiments indicate the potential of tuber flour of white sweet potato to heal wounds. PMID:25685447

  20. Effects of heat stress on the endogenous contents of hormones and related gene expression in developing axillary tubes of potato

    Science.gov (United States)

    Potatoes are a cool season crop and optimum tuber initiation, development, yield, and quality require growing temperatures between 15 -20°C. At temperatures above 30°C, tuber initiation is delayed or prevented and developing tubers exhibit decreased starch content, increased malformation, chain-tube...

  1. Heat toxicant contaminant mitigation in potato chips

    DEFF Research Database (Denmark)

    Mariotti, Maria; Cortes, Pablo; Fromberg, Arvid

    2015-01-01

    Heating foods immersed in oil during frying provides many attractive sensorial attributes including taste, flavor and color. However, some toxic compounds formed during frying of potatoes such as furan and acrylamide may constitute an increased cancer risk for consumers. The objective of this work...... and acrylamide generation in thermally treated starchy foods. A central composite design was implemented to optimize the temperature-time blanching conditions under which furan, acrylamide and oil content in potato chips were minimized. The optimum blanching conditions were 64 degrees C and 17 min in which...

  2. Eradication of sweet potato weevil using Co-60 gamma radiation

    International Nuclear Information System (INIS)

    Tokunaga, Taizo

    2007-01-01

    Sweet potato weevil which is a harmful insect injuring sweet potatoes was found out at Yoron Island in 1915 for the first time in Kagoshima prefecture, Japan. Here the eradication of sweet potato weevils using cobalt 60 irradiation achieved at Kikai Island is described. The mass-reared male weevils in potatoes are in pasture after sterilized by gamma irradiation. If the sexually sterile male copulates with a wild female, the egg does not incubate. By the repeated sterilization during several generations, the eradication of sweet potato weevils was accomplished. (M.H.)

  3. Effect of radiation on potato susceptibility to plant diseases

    International Nuclear Information System (INIS)

    Younis, N.A.

    1982-01-01

    The average world production of potatoes is about 7 billion bushels, having a value nearly, if not quite, equal to that of wheat. Hence, it has for years been recognized as the world's leading food crop (Thompson, 1949). Potato is the world's leading vegetable crop and rivals wheat in total value. There is probably no food article in daily diet of the white race more common than the potato. Potato tuber contains nearly 80 per cent water in it's uncooked state. Most of the remainder consists of about 2 per cent protein and 18 per cent starch. The potato is one of the cheapest and most common sources of carbohydrate food

  4. Effect of tuber skin on the thermal properties of whole tubers of potato and sweet potato

    Science.gov (United States)

    Oluwo, A. A.; Khan, R. M.; Salami, M. J. E.

    2013-12-01

    Temperature-dependent thermal coefficients of mathematical models of the postharvest storage process play an important role in determining the models accuracy. Thermal properties of tubers under storage available in literature are generally of those in semi processed form (skinless) such as those having undergone peeling, dicing and cutting actions. This study investigates the effect of tuber skin on the thermal properties of whole tubers of potato and sweet potato. A direct approach was used to measure the tubers' density and thermal conductivity and thermal diffusivity by the transient heat transfer method. Indirect approach was used to measure the tubers' specific heat. Experimental data were used to develop empirical models of the thermal coefficients as a function of temperature. Results of the study should find great use in the modeling of potato and sweet potato storage process.

  5. Selenium Supplementation of Amaranth Sprouts Influences Betacyanin Content and Improves Anti-Inflammatory Properties via NF?B in Murine RAW 264.7 Macrophages

    OpenAIRE

    Tyszka-Czochara, Malgorzata; Pasko, Pawel; Zagrodzki, Pawel; Gajdzik, Ewelina; Wietecha-Posluszny, Renata; Gorinstein, Shela

    2015-01-01

    Sprouts contain potent compounds which while influencing crucial transduction pathways in cell reveal anti-inflammatory and anticancer activities. In this study, we report the biological activity for seeds and colourful sprouts of four types of edible amaranth, as amaranth has recently attracted interest due to its appreciable nutritional value. MTT assay conducted for the amaranth seeds and sprouts did not show any adverse effect on the viability of murine RAW 264.7 cells. As amaranth accumu...

  6. Comparison of Listeria monocytogenes recoveries from spiked mung bean sprouts by the enrichment methods of three regulatory agencies.

    Science.gov (United States)

    Cauchon, Kaitlin E; Hitchins, Anthony D; Smiley, R Derike

    2017-09-01

    Three selective enrichment methods, the United States Food and Drug Administration's (FDA method), the United States Department of Agriculture Food Safety Inspection Service's (USDA method), and the EN ISO 11290-1 standard method, were assessed for their suitability for recovery of Listeria monocytogenes from spiked mung bean sprouts. Three parameters were evaluated; the enrichment L. monocytogenes population from singly-spiked sprouts, the enrichment L. monocytogenes population from doubly-spiked (L. monocytogenes and Listeria innocua) sprouts, and the population differential resulting from the enrichment of doubly-spiked sprouts. Considerable L. monocytogenes inter-strain variation was observed. The mean enrichment L. monocytogenes populations for singly-spiked sprouts were 6.1 ± 1.2, 4.9 ± 1.2, and 6.9 ± 2.3 log CFU/mL for the FDA, USDA, and EN ISO 11290-1 methods, respectively. The mean L. monocytogenes populations for doubly-spiked sprouts were 4.7 ± 1.1, 5.5 ± 1.3, and 4.6 ± 1.4 log CFU/mL for the FDA, USDA, and ISO 11290-1 enrichment methods, respectively. The corresponding mean population differentials were 2.8 ± 1.1, 3.3 ± 1.3, and 3.6 ± 1.4 Δlog CFU/mL for the same three enrichment methods, respectively. The presence of L. innocua and resident microorganisms on the sprouts negatively impacted final levels of L. monocytogenes with all three enrichment methods. Published by Elsevier Ltd.

  7. Antioxidant capacity, phenolic and vitamin C contents of quinoa (Chenopodium quinoa Willd. as affected by sprouting and storage conditions

    Directory of Open Access Journals (Sweden)

    Maura N. Laus

    2017-03-01

    Full Text Available Antioxidant capacity (AC of quinoa (Chenopodium quinoa Willd. cv. Real seeds and sprouts obtained after 4 days of seed germination at 20°C and 70% humidity was evaluated using trolox equivalent antioxidant capacity (TEAC and oxygen radical absorbance capacity (ORAC assays, able to highlight reducing activity and peroxyl radical scavenging capacity, respectively; phenolic content (PC was also measured. Both TEAC and ORAC assays revealed a significantly higher (about 2- and 2.8-fold, respectively AC of 4-day-old sprouts compared to seeds; consistently, also PC values of sprouts resulted about 2.6 times higher than seeds. In order to investigate the influence of storage on AC and PC, as well as on vitamin C content (VCC, 4-day-old sprouts were subjected for 7 days at 5°C to three different conditions of controlled atmosphere storage (CAS compared with air. Interestingly, whatever the CAS conditions, storage of quinoa sprouts up to 7 days induced an increase of AC evaluated in terms of reducing activity by TEAC assay. Consistently, an increase of PC and VCC was measured during storage, positively correlated to TEAC values. Moreover, a decrease of peroxyl radical scavenging activity, measured by ORAC, was observed after 7 days of storage, in accordance with a shift of AC towards the reducing activity component. Overall, these findings indicate that sprouting approach using quinoa may provide highly antioxidant-enriched seedlings that may improve nutritional quality of diet or of functional foods. Interestingly, antioxidant properties of quinoa sprouts may be deeply influenced by storage, able to increase reducing activity by increasing phenols and vitamin C.

  8. The effect of calcinated calcium and chlorine treatments on Escherichia coli O157:H7 87-23 population reduction in radish sprouts.

    Science.gov (United States)

    Fransisca, Lilia; Zhou, Bin; Park, Heekyung; Feng, Hao

    2011-08-01

    The effect of calcinated calcium spray on Escherichia coli O157:H7 87-23 population reduction during radish sprout production was studied. Artificially inoculated radish seeds were soaked in sodium hypochlorite (NaOCl) solutions (200 and 20000 ppm), rinsed in distilled water, and sprayed with water or a calcinated calcium solution during sprouting. Microbial plate count was obtained at each step of the process and germination rate was determined after 72 h of sprouting. Scanning electron microscopy (SEM) was done on treated seeds and sprouts to locate which parts were populated by the E. coli cells. The results showed that the active compound in the calcinated calcium was calcium oxide. The treatment of 200 ppm NaOCl soaking followed by 0.04% calcinated calcium spray resulted in no microbial growth after a 72-h sprouting, while maintaining a high germination rate. The 0.4% calcinated calcium spray significantly reduced the germination rate and is therefore not recommended. Soaking the seeds in a 20000 ppm chlorine solution achieved the highest E. coli count reduction (1.65 log CFU/g). However, the E. coli cells that survived the 20000 ppm chlorine soak grew to 6 log CFU/g sprouts after a 72-h sprouting, significantly higher than the initial count on the seeds. The SEM microimages showed that the bacteria were mostly located in the roots of the radish sprouts and all across the seed surface. The E. coli O157:H7 87-23 cells appeared to be located in biofilms or embedded into the radish sprout tissues during sprouting. The seed sanitation treatment with 20000 ppm chlorine solution that is currently used by the sprout industry was once again found to be ineffective in eliminating inoculated pathogenic cells. More importantly, the remaining cells that have survived the chlorine wash would grow during sprouting to reach an alarmingly high cell concentration. The new observation of E. coli cells and sprout tissue interaction manifested as embedding

  9. Assessment of the anticancer compounds Se-methylselenocysteine and glucosinolates in Se-biofortified broccoli (Brassica oleracea L. var. italica) sprouts and florets.

    Science.gov (United States)

    Ávila, Fabricio William; Faquin, Valdemar; Yang, Yong; Ramos, Silvio Junio; Guilherme, Luiz Roberto G; Thannhauser, Theodore W; Li, Li

    2013-07-03

    Broccoli (Brassica oleracea L. var. italica) is a rich source of chemopreventive compounds. Here, we evaluated and compared the effect of selenium (Se) treatment on the accumulation of anticancer compounds Se-methylselenocysteine (SeMSCys) and glucosinolates in broccoli sprouts and florets. Total Se and SeMSCys content in sprouts increased concomitantly with increasing Se doses. Selenate was superior to selenite in inducing total Se accumulation, but selenite is equally effective as selenate in promoting SeMSCys synthesis in sprouts. Increasing sulfur doses reduced total Se and SeMSCys content in sprouts treated with selenate, but not in those with selenite. Examination of five broccoli cultivars reveals that sprouts generally have better fractional ability than florets to convert inorganic Se into SeMSCys. Distinctive glucosinolate profiles between sprouts and florets were observed, and sprouts contained approximately 6-fold more glucoraphanin than florets. In contrast to florets, glucosinolate content was not affected by Se treatment in sprouts. Thus, Se-enriched broccoli sprouts are excellent for simultaneous accumulation of chemopreventive compounds SeMSCys and glucoraphanin.

  10. Does inter-plant variation in sprouting time affect the growth/reproduction trade-off and herbivory in a tropical tree species?

    Directory of Open Access Journals (Sweden)

    Marcilio Fagundes

    Full Text Available ABSTRACT The timing of phenological events varies within and among populations, affecting the performance of individual plants differently. We evaluated the effects of relative variation in sprouting time on the display of reproductive events, vegetative growth and herbivory in Copaifera langsdorffii (Fabaceae. A total of 93 trees of C. langsdorffii was monitored daily to determine their sprouting time. We collected ten terminal branches of each plant to evaluate vegetative growth, production of defense compounds and insect herbivore damage. The sprouting time for the studied population lasted 67 days. Variation in sprouting time did not affect the probability of plants to enter the reproductive stage. Plants that entered the reproductive stage showed greater vegetative biomass. Variation in sprouting time had a negative relationship with branch growth and a positive relationship with the number of leaflets. Leaf phenol concentration did not vary in relation to sprouting time or plant phenology, but herbivory was higher in plants that sprouted later. The relationships among plant sprouting time, vegetative development and display of reproductive stage in C. langsdorffii are discussed. The results of this study also suggest that early sprouting prior to the rainy season is a strategy used by C. langsdorffii to escape herbivores attacks.

  11. Expressing the sweet potato orange gene in transgenic potato improves drought tolerance and marketable tuber production.

    Science.gov (United States)

    Cho, Kwang-Soo; Han, Eun-Heui; Kwak, Sang-Soo; Cho, Ji-Hong; Im, Ju-Seong; Hong, Su-Young; Sohn, Hwang-Bae; Kim, Yun-Hee; Lee, Shin-Woo

    2016-01-01

    Potato (Solanum tuberosum L.) is generally considered to be sensitive to drought stress. Even short periods of water shortage can result in reduced tuber production and quality. We previously reported that transgenic potato plants expressing the sweet potato orange gene (IbOr) under the control of the stress-inducible SWPA2 promoter (referred to as SOR plants) showed increased tolerance to methyl viologen-mediated oxidative stress and high salinity, along with increased carotenoid contents. In this study, in an effort to improve the productivity and environmental stress tolerance of potato, we subjected transgenic potato plants expressing IbOr to water-deficient conditions in the greenhouse. The SOR plants exhibited increased tolerance to drought stress under greenhouse conditions. IbOr expression was associated with slightly negative phenotypes, including reduced tuber production. Controlling IbOr expression imparted the same degree of drought tolerance while ameliorating these negative phenotypic effects, leading to levels of tuber production similar to or better than those of wild-type plants under drought stress conditions. In particular, under drought stress, drought tolerance and the production of marketable tubers (over 80g) were improved in transgenic plants compared with non-transgenic plants. These results suggest that expressing the IbOr transgene can lead to significant gains in drought tolerance and tuber production in potato, thereby improving these agronomically important traits. Copyright © 2016 Académie des sciences. Published by Elsevier SAS. All rights reserved.

  12. The effect of gamma-irradiation on the sucrose content in sweet potato roots and potato tubers

    International Nuclear Information System (INIS)

    Hayashi, T.; Kawashima, K.

    1982-01-01

    The sucrose content in both potato tubers and sweet potato roots was considerably increased by gamma-irradiation. The maximum increase was achieved by an irradiation dose of 3 to 4 kGy for potatoes and 0.8 to 2 kGy for sweet potatoes. Cooling treatment (15°C, 2 weeks) for sweet potato roots also enhanced the sucrose content (almost 2 times) but was not additive to the irradiation treatment; the maximum sucrose content in irradiated sweet potato roots was in the range of 7 to 12% irrespective of the cooling treatment, depending on the variety of sweet potatoes. Irradiation made the sucrose content in the roots 2 to 4 times higher

  13. Crop growth model WOFOST applied to potatoes

    NARCIS (Netherlands)

    Koning, de G.H.J.; Diepen, van C.A.; Reinds, G.J.

    1995-01-01

    The WOFOST model was calibrated with an experiment on yield effects of drought in potatoes, using data on weather, soil moisture and crop calendar. Then, crop growth and development were predicted for the next year, using planting date and weather data. The model is described. The adjustments in the

  14. ( amala ) made from sweet potato flour ( elubo )

    African Journals Online (AJOL)

    Describing the sensory characteristics of new or modified products is an integral part of food quality control. Sweet potato amala as an important end product could serve as an avenue for utilization of the crop, however, sensory attributes that will influence and ensure consumer acceptability need to be determined.

  15. THE PERFORMANCE AND PROFITABILITY OF SWEET POTATO ...

    African Journals Online (AJOL)

    ABSTRACT. This study was conducted in 2013 and 2014 at the Teaching and Research Farm of the Faculty of. Agriculture, University of Benin, Benin City, Nigeria. The aim of the study was to evaluate the effect of propagule length and cattle dung application rates on the growth, yield and profitability of sweet potato.

  16. Responses of potatoes plants inoculated with arbuscular ...

    African Journals Online (AJOL)

    A pot experiment was set to examine the impact of the foliar litter (Hardwickia binata and Azadirachta indica) and an arbuscular mycorrhizal (AM) fungus on the development of two varieties of potato plants (Aida, Atlas). Three litter doses (0, 25 and 50 g) were applied to the pots after bedding plantlets. The plants were ...

  17. Leaf wetness distribution within a potato crop

    Science.gov (United States)

    Heusinkveld, B. G.

    2010-07-01

    The Netherlands has a mild maritime climate and therefore the major interest in leaf wetness is associated with foliar plant diseases. During moist micrometeorological conditions (i.e. dew, fog, rain), foliar fungal diseases may develop quickly and thereby destroy a crop quickly. Potato crop monocultures covering several hectares are especially vulnerable to such diseases. Therefore understanding and predicting leaf wetness in potato crops is crucial in crop disease control strategies. A field experiment was carried out in a large homogeneous potato crop in the Netherlands during the growing season of 2008. Two innovative sensor networks were installed as a 3 by 3 grid at 3 heights covering an area of about 2 hectares within two larger potato crops. One crop was located on a sandy soil and one crop on a sandy peat soil. In most cases leaf wetting starts in the top layer and then progresses downward. Leaf drying takes place in the same order after sunrise. A canopy dew simulation model was applied to simulate spatial leaf wetness distribution. The dew model is based on an energy balance model. The model can be run using information on the above-canopy wind speed, air temperature, humidity, net radiation and within canopy air temperature, humidity and soil moisture content and temperature conditions. Rainfall was accounted for by applying an interception model. The results of the dew model agreed well with the leaf wetness sensors if all local conditions were considered. The measurements show that the spatial correlation of leaf wetness decreases downward.

  18. Internal Ballistics of a Pneumatic Potato Cannon

    Science.gov (United States)

    Mungan, Carl E.

    2009-01-01

    Basic laws of thermodynamics and mechanics are used to analyse an air gun. Such devices are often employed in outdoor physics demonstrations to launch potatoes using compressed gas that is here assumed to expand reversibly and adiabatically. Reasonable agreement is found with reported muzzle speeds for such homebuilt cannons. The treatment is…

  19. Potato Types and Characteristics: Laboratory Exercises.

    Science.gov (United States)

    Pavlista, Alexander D.

    1997-01-01

    Presents a number of simple exercises that demonstrate potato tuber characteristics and are designed for high school biology students and teachers. Exercises include Typing, Grading, Shape, Eye Characteristics, Defects, Specific Gravity, Dry Matter Content, Glucose Content, Baking, Frying/Chipping, and Taste Testing. (JRH)

  20. Carbohydrates and gibberellins relationship in potato tuberization

    Czech Academy of Sciences Publication Activity Database

    Ševčíková, H.; Mašková, P.; Tarkowská, Danuše; Mašek, T.; Lipavská, H.

    2017-01-01

    Roč. 214, JUL (2017), s. 53-63 ISSN 0176-1617 R&D Projects: GA ČR GA14-34792S Institutional support: RVO:61389030 Keywords : Carbohydrate distribution * Gibberellin * Photoautotrophic cultivation * Potato * Tuberization Subject RIV: CB - Analytical Chemistry, Separation OBOR OECD: Plant sciences, botany Impact factor: 3.121, year: 2016

  1. Electroantennogram responses of the potato tuber moth ...

    Indian Academy of Sciences (India)

    PRAKASH

    Compounds belonging to the fatty acid derivatives class appear to be important for an oligophagous pest such as the potato tuber moth and the findings are discussed in relation to host plant selection in this species. [Das P D, Raina R, ..... The ability of both males and females to detect the odours presented is probably due ...

  2. Potato developments in a changing Europe

    NARCIS (Netherlands)

    Haase, N.U.; Haverkort, A.J.

    2006-01-01

    The papers in this book reflect societal and commercial changes such as consumer behaviour and marketing aspects in relation to fresh and processed potatoes in western, central and eastern Europe. Seed trade between western and central Europe is entering a new stage with altered inspection

  3. Analysis of chromosome termini in potato varieties

    Czech Academy of Sciences Publication Activity Database

    Fajkus, Jiří; Novotná, Marcela; Ptáček, J.

    2002-01-01

    Roč. 48, č. 11 (2002), s. 477-479 ISSN 0370-663X R&D Projects: GA MZe QC1164; GA ČR GA204/02/0027 Institutional research plan: CEZ:AV0Z5004920 Keywords : plant chromosomes * telomere lenght * potato varieties Subject RIV: BO - Biophysics Impact factor: 0.333, year: 2002

  4. Sweet Potato Ketchup: Feasibility, Acceptability, and Production ...

    African Journals Online (AJOL)

    Ketchup sauce is increasingly a popular condiment used as a flavouring ingredient in fast-food businesses in East African urban areas. It is one of a myriad of products that can be made using sweet potato (Ipomoea batatas) roots. We assessed the feasibility, consumer acceptability, and cost of production for a ketchup ...

  5. Purple sweet potato color attenuates domoic acid-induced cognitive deficits by promoting estrogen receptor-α-mediated mitochondrial biogenesis signaling in mice.

    Science.gov (United States)

    Lu, Jun; Wu, Dong-Mei; Zheng, Yuan-Lin; Hu, Bin; Cheng, Wei; Zhang, Zi-Feng

    2012-02-01

    Recent findings suggest that endoplasmic reticulum stress may be involved in the pathogenesis of domoic acid-induced neurodegeneration. Purple sweet potato color, a class of naturally occurring anthocyanins, has beneficial health and biological effects. Recent studies have also shown that anthocyanins have estrogenic activity and can enhance estrogen receptor-α expression. In this study, we evaluated the effect of purple sweet potato color on cognitive deficits induced by hippocampal mitochondrial dysfunction in domoic acid-treated mice and explored the potential mechanisms underlying this effect. Our results showed that the oral administration of purple sweet potato color to domoic acid-treated mice significantly improved their behavioral performance in a step-through passive avoidance task and a Morris water maze task. These improvements were mediated, at least in part, by a stimulation of estrogen receptor-α-mediated mitochondrial biogenesis signaling and by decreases in the expression of p47phox and gp91phox. Decreases in reactive oxygen species and protein carbonylation were also observed, along with a blockade of the endoplasmic reticulum stress pathway. Furthermore, purple sweet potato color significantly suppressed endoplasmic reticulum stress-induced apoptosis, which prevented neuron loss and restored the expression of memory-related proteins. However, knockdown of estrogen receptor-α using short hairpin RNA only partially blocked the neuroprotective effects of purple sweet potato color in the hippocampus of mice cotreated with purple sweet potato color and domoic acid, indicating that purple sweet potato color acts through multiple pathways. These results suggest that purple sweet potato color could be a possible candidate for the prevention and treatment of cognitive deficits in excitotoxic and other brain disorders. Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved.

  6. Acrylamide mitigation in potato chips by using NaCl

    DEFF Research Database (Denmark)

    Pedreschi, Franco; Risum, Jørgen; Granby, Kit

    2009-01-01

    % NaCI solution at 25 °) for 5 min; (iv) Blan ching in hot water at 90°) for 5 min plus immersion in a distilled water at 25 °) for 5 min. Raw potato slices were used as control. Blanching reduced the acrylamide content of potato chips with 12 %. On the other hand, when potato slices were immersed...... potato chips and French fries, at levels of 30-2300 ppb. The objective of this research was to study the effect of immersing potato slices in a NaCI solution in relation to acrylamide formation in the prepared potato chips. Potato slices (Verdi variety, diameter: 40 mm, width: 2.0 mm) were fried at 170...... in salt solution, the acrylamide only decreased with 3 %. Interestingly, when blanched potato slices were immersed in NaCl solution and then fried, the acrylamide concentrations of the potato chips decreased with 66 %. Blanching seems to make the NaCI diffusion in potato tissue easier leading...

  7. Potential impacts of bioprocessing of sweet potato: Review.

    Science.gov (United States)

    El Sheikha, Aly Farag; Ray, Ramesh C

    2017-02-11

    Sweet potato (Ipomoea batatas L.) is among the major food crops in the world and is cultivated in all tropical and subtropical regions particularly in Asia, Africa, and the Pacific. Asia and Africa regions account for 95% of the world's production. Among the root and tuber crops grown in the world, sweet potato ranks second after cassava. In previous decades, sweet potato represented food and feed security, now it offers income generation possibilities, through bioprocessing products. Bioprocessing of sweet potato offers novel opportunities to commercialize this crop by developing a number of functional foods and beverages such as sour starch, lacto-pickle, lacto-juice, soy sauce, acidophilus milk, sweet potato curd and yogurt, and alcoholic drinks through either solid state or submerged fermentation. Sweet potato tops, especially leaves are preserved as hay or silage. Sweet potato flour and bagassae are used as substrates for production of microbial protein, enzymes, organic acids, monosodium glutamate, chitosan, etc. Additionally, sweet potato is a promising candidate for production of bioethanol. This review deals with the development of various products from sweet potato by application of bioprocessing technology. To the best of our knowledge, there is no review paper on the potential impacts of the sweet potato bioprocessing.

  8. Effect of ascorbic acid postharvest treatment on enzymatic browning, phenolics and antioxidant capacity of stored mung bean sprouts.

    Science.gov (United States)

    Sikora, Małgorzata; Świeca, Michał

    2018-01-15

    Enzymatic browning limits the postharvest life of minimally processed foods, thus the study selected the optimal inhibitors of polyphenol oxidase (PPO) and evaluated their effect on enzymatic browning, phenolics and antioxidant capacity of stored mung bean sprouts. The sprouts treated with 2mM and 20mM ascorbic acid had a lowered PPO activity; compared to the control by 51% and 60%, respectively. The inhibition was reflected in a significant decrease in enzymatic browning. The sprouts treated with 20mM ascorbic acid had 22% and 23% higher phenolic content after 3 and 7days of storage, respectively. Both storage and ascorbic acid treatment increased potential bioaccessibility of phenolics. Generally, there was no effect of the treatments on the antioxidant capacity; however, a significant increase in the reducing potential was determined for the sprouts washed with 20mM ascorbic acid. In conclusion, ascorbic acid treatments may improve consumer quality of stored sprouts. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Effect of Cooking on Isoflavones, Phenolic Acids, and Antioxidant Activity in Sprouts of Prosoy Soybean (Glycine max).

    Science.gov (United States)

    Kumari, Shweta; Chang, Sam K C

    2016-07-01

    Soy sprouts possess health benefits and is required to be cooked before consumption. The effects of cooking on the phenolic components and antioxidant properties of soy sprouts with different germination days were investigated. A food-grade cultivar Prosoy with a high protein content was germinated for 1, 2, 3, 5, and 7 d and cooked till palatable for 20, 20, 5, 5, and 7 min, respectively. Total phenolic content (TPC), total flavonoids content (TFC), condensed tannins content (CTC), individual phenolic acids, isoflavones, DPPH, ferric-reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) of raw and cooked sprouts were measured. Cooking caused significant losses in phenolic content and antioxidant activities, and maximum loss was on day 3 > 5 > 7, including TPC (32%, 23%, and 15%), TFC (50%, 44%, and 20%), CTC (73%, 47%, and 12%), DPPH (31%, 15%, and 5%), FRAP (34%, 25%, and 1%), and ORAC (34%, 22%, 32%), respectively. Cooking caused significant losses in most individual phenolic acid, benzoic group, cinnamic group, total phenolic composition, individual isoflavones, and total isoflavones. The losses of phenolic acids such as gallic, protocatechuic, hydroxybenzoic, syringic, chlorogenic, or sinapic acids during cooking were not compensated by the increases in trihydroxybenzoic, vanillic or coumaric acids on certain days of germination. Cooking caused minimal changes in phenolic acid composition of day 1 and 2 sprouts compared to 3, 5, and 7 d sprouts. © 2016 Institute of Food Technologists®

  10. Ciliary neurotrophic factor is not required for terminal sprouting and compensatory reinnervation of neuromuscular synapses: re-evaluation of CNTF null mice.

    Science.gov (United States)

    Wright, Megan C; Son, Young-Jin

    2007-06-01

    Loss of synaptic activity or innervation induces sprouting of intact motor nerve terminals that adds or restores nerve-muscle connectivity. Ciliary neurotrophic factor (CNTF) and terminal Schwann cells (tSCs) have been implicated as molecular and cellular mediators of the compensatory process. We wondered if the previously reported lack of terminal sprouting in CNTF null mice was due to abnormal reactivity of tSCs. To this end, we examined nerve terminal and tSC responses in CNTF null mice using experimental systems that elicited extensive sprouting in wildtype mice. Contrary to the previous report, we found that motor nerve terminals in the null mice sprout extensively in response to major sprouting-stimuli such as exogenously applied CNTF per se, botulinum toxin-elicited paralysis, and partial denervation by L4 spinal root transection. In addition, the number, length and growth patterns of terminal sprouts, and the extent of reinnervation by terminal or nodal sprouts, were similar in wildtype and null mice. tSCs in the null mice were also reactive to the sprouting-stimuli, elaborating cellular processes that accompanied terminal sprouts or guided reinnervation of denervated muscle fibers. Lastly, CNTF was absent in quiescent tSCs in intact, wildtype muscles and little if any was detected in reactive tSCs in denervated muscles. Thus, CNTF is not required for induction of nerve terminal sprouting, for reactivation of tSCs, and for compensatory reinnervation after nerve injury. We interpret these results to support the notion that compensatory sprouting in adult muscles is induced primarily by contact-mediated mechanisms, rather than by diffusible factors.

  11. Untargeted Metabolomics Reveals Predominant Alterations in Lipid Metabolism Following Light Exposure in Broccoli Sprouts

    Directory of Open Access Journals (Sweden)

    Mariateresa Maldini

    2015-06-01

    Full Text Available The consumption of vegetables belonging to the family Brassicaceae (e.g., broccoli and cauliflower is linked to a reduced incidence of cancer and cardiovascular diseases. The molecular composition of such plants is strongly affected by growing conditions. Here we developed an unbiased metabolomics approach to investigate the effect of light and dark exposure on the metabolome of broccoli sprouts and we applied such an approach to provide a bird’s-eye view of the overall metabolic response after light exposure. Broccoli seeds were germinated and grown hydroponically for five days in total darkness or with a light/dark photoperiod (16 h light/8 h dark cycle. We used an ultra-performance liquid-chromatography system coupled to an ion-mobility, time-of-flight mass spectrometer to profile the large array of metabolites present in the sprouts. Differences at the metabolite level between groups were analyzed using multivariate statistical analyses, including principal component analysis and correlation analysis. Altered metabolites were identified by searching publicly available and in-house databases. Metabolite pathway analyses were used to support the identification of subtle but significant changes among groups of related metabolites that may have gone unnoticed with conventional approaches. Besides the chlorophyll pathway, light exposure activated the biosynthesis and metabolism of sterol lipids, prenol lipids, and polyunsaturated lipids, which are essential for the photosynthetic machinery. Our results also revealed that light exposure increased the levels of polyketides, including flavonoids, and oxylipins, which play essential roles in the plant’s developmental processes and defense mechanism against herbivores. This study highlights the significant contribution of light exposure to the ultimate metabolic phenotype, which might affect the cellular physiology and nutritional value of broccoli sprouts. Furthermore, this study highlights the

  12. Replacing corn with pearl millet (raw and sprouted) with and without enzyme in chickens' diet.

    Science.gov (United States)

    Afsharmanesh, M; Ghorbani, N; Mehdipour, Z

    2016-04-01

    An experiment was conducted to compare a commercial corn-soya bean meal diet with a pearl millet (raw and sprouted) diet containing less soya bean meal, alone or in combination with exogenous enzyme, on growth performance and ileal villus development of chicks. Two-hundred-and-forty-one-day-old male broilers (10/pen) were randomly allocated to one of the following dietary treatments: (i) a standard corn-soya bean meal control diet (CTL); (ii) a raw pearl millet-soya bean meal diet (PM); (iii) a sprouted pearl millet-soya bean meal diet (SPM); (iv) CTL + exogenous enzymes (CE); (v) PM + exogenous enzymes (PE); and (vi) SPM + exogenous enzymes (SPE) with four replicate pens/treatment. Body weight of birds at day 21 did not differ between those fed the CTL, and SPM and PE diets. In comparison with feeding broilers the CTL diet, feeding the PE and SPM diets caused significant decrease in feed intake, but with equivalent growth and feed efficiency. However, at day 21, feed conversion ratio did not differ between birds fed the CTL diet and those fed the PM, PE and SPM diets. At day 21, broilers fed the PM and PE diets had longer villi (p diet. At day 21, villi width was reduced (p diet. It is concluded that, in comparison with corn, broiler diets formulated with sprouted pearl millet or pearl millet with enzyme require less soya bean meal and can be used to improve growth performance traits and villus development. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  13. A Heterogeneous In Vitro Three Dimensional Model of Tumour-Stroma Interactions Regulating Sprouting Angiogenesis

    Science.gov (United States)

    Correa de Sampaio, Pedro; Auslaender, David; Krubasik, Davia; Failla, Antonio Virgilio; Skepper, Jeremy N.; Murphy, Gillian; English, William R.

    2012-01-01

    Angiogenesis, the formation of new blood vessels, is an essential process for tumour progression and is an area of significant therapeutic interest. Different in vitro systems and more complex in vivo systems have been described for the study of tumour angiogenesis. However, there are few human 3D in vitro systems described to date which mimic the cellular heterogeneity and complexity of angiogenesis within the tumour microenvironment. In this study we describe the Minitumour model – a 3 dimensional human spheroid-based system consisting of endothelial cells and fibroblasts in co-culture with the breast cancer cell line MDA-MB-231, for the study of tumour angiogenesis in vitro. After implantation in collagen-I gels, Minitumour spheroids form quantifiable endothelial capillary-like structures. The endothelial cell pre-capillary sprouts are supported by the fibroblasts, which act as mural cells, and their growth is increased by the presence of cancer cells. Characterisation of the Minitumour model using small molecule inhibitors and inhibitory antibodies show that endothelial sprout formation is dependent on growth factors and cytokines known to be important for tumour angiogenesis. The model also shows a response to anti-angiogenic agents similar to previously described in vivo data. We demonstrate that independent manipulation of the different cell types is possible, using common molecular techniques, before incorporation into the model. This aspect of Minitumour spheroid analysis makes this model ideal for high content studies of gene function in individual cell types, allowing for the dissection of their roles in cell-cell interactions. Finally, using this technique, we were able to show the requirement of the metalloproteinase MT1-MMP in endothelial cells and fibroblasts, but not cancer cells, for sprouting angiogenesis. PMID:22363483

  14. Effect of heat treatment to sweet potato flour on dough properties and characteristics of sweet potato-wheat bread.

    Science.gov (United States)

    Pérez, Isela Carballo; Mu, Tai-Hua; Zhang, Miao; Ji, Lei-Lei

    2017-12-01

    The effect of heat treatment at 90, 100, 110 and 120 ℃ for 20 min to sweet potato flour on dough properties and characteristics of sweet potato-wheat bread was investigated. The lightness (L*) and a* of sweet potato flour samples after heat treatment were increased, while the b* were decreased significantly, as well as the particle size, volume and area mean diameter ( p sweet potato flour was observed, where the number of irregular granules increased as the temperature increased from 90 to 120 ℃. Compared with sweet potato flour samples without heat treatment and with heat treatment at 90, 100 and 120 ℃, the gelatinization temperature and enthalpy change of sweet potato flour at 110 ℃ were the lowest, which were 77.94 ℃ and 3.67 J/g, respectively ( p sweet potato flour increased significantly from 1199 ml without heat treatment to 1214 ml at 90 ℃ ( p sweet potato-wheat bread with sweet potato flour after heat treatment increased significantly, which was the largest at 90 ℃ (2.53 cm 3 /g) ( p sweet potato flour could be potentially used in wheat bread production.

  15. Change in particle size of pectin reacted with pectinesterase isozymes from pea (Pisum sativum L.) sprout.

    Science.gov (United States)

    Jiang, C M; Wu, M C; Chang, W H; Chang, H M

    2001-09-01

    Four pectinesterase (PE) isozymes were isolated by CM-Sepharose CL-6B chromatography from etiolated pea (Pisum sativum L.) sprouts and then reacted with citrus pectin (degree of esterification = 68%, 30-100 kDa) to observe the change in pectin particle size using a laser particle size analyzer. After incubation of a pectin-PE mixture (pH 6.5) at 30 degrees C for 4 h, PE 1 was observed to catalyze the transacylation reaction most remarkably, increasing the particle size from approximately 50-70 to approximately 250-350 nm, followed by PE 3, PE 2, and PE 4.

  16. Spray-Dried Potato Juice as a Potential Functional Food Component with Gastrointestinal Protective Effects

    Directory of Open Access Journals (Sweden)

    Małgorzata Kujawska

    2018-02-01

    Full Text Available Background: Peptic ulcer disease, including its complications and functional dyspepsia, are prevalent gastrointestinal diseases, etiopathogenesis of which is associated with mucosal inflammation. Research into new therapeutics capable of preventing or curing gastrointestinal mucosal damage has been steadily developing over past decades. This study was undertaken to evaluate whether a spray-dried preparation of potato juice is applicable for treating and preventing gastrointestinal mucosal damage. Methods: We assessed potential protective effects of spray-dried potato juice (SDPJ against gut inflammation in the co-culture Caco-2/RAW264.7 system, as well as a gastroprotective activity in a rat model of gastric ulceration. Results: The obtained results indicated that SDPJ down-regulates lipopolysaccharide (LPS-induced mRNA expression and protein production of proinflammatory cytokines IL-6 and TNF-α in the co-culture model. Moreover, SDPJ provided dose-dependent protection against LPS-induced disruption of intestinal barrier integrity. In rats, five-day pretreatment with SDPJ in doses of 200 mg/kg and 500 mg/kg suppressed HCl/ethanol-induced TNF-α expression in gastric mucosa by 52% and 35%, respectively. In addition, the pretreatment with the lower dose of SDPJ reduced the incidence of ulcers (by 34% expressed as ulcer index. Conclusion: The spray-dried potato juice appears to be an attractive candidate for ameliorating inflammation-related diseases of the gastrointestinal tract.

  17. Verification of presence of caprolactam in sprouted achenes of Fagopyrum esculentum Moench and its influence on plant phenolic compound content.

    Science.gov (United States)

    Kalinová, Jana P; Tříska, Jan; Vrchotová, Naděžda; Moos, Martin

    2014-08-15

    The presence of caprolactam, a precursor of Nylon-6, among those synthetic polymers which are widely-spread throughout the environment, could be the reason for its being found in plants. The aim of this work was to confirm the previously described presence of caprolactam in dry and sprouted achenes, as well as in achene exudates of common buckwheat (Fagopyrum esculentum Moench). When the lyophilized sprouted and dry buckwheat achenes, along with exudates from growth experiments, with caprolactam-free medium were analysed by HPLC, no caprolactam was found. After addition of caprolactam into the growth medium, we confirmed the uptake of caprolactam in the lyophilized sprouted buckwheat achenes. The uptake of caprolactam is also a function of light conditions during the growth experiments. Caprolactam also inhibits the content of phenolic compounds; especially rutin, vitexin, isovitexin, orientin, and homoorientin in buckwheat plants. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. International outbreak of multiple Salmonella serotype infections linked to sprouted chia seed powder - USA and Canada, 2013-2014.

    Science.gov (United States)

    Harvey, R R; Heiman Marshall, K E; Burnworth, L; Hamel, M; Tataryn, J; Cutler, J; Meghnath, K; Wellman, A; Irvin, K; Isaac, L; Chau, K; Locas, A; Kohl, J; Huth, P A; Nicholas, D; Traphagen, E; Soto, K; Mank, L; Holmes-Talbot, K; Needham, M; Barnes, A; Adcock, B; Honish, L; Chui, L; Taylor, M; Gaulin, C; Bekal, S; Warshawsky, B; Hobbs, L; Tschetter, L R; Surin, A; Lance, S; Wise, M E; Williams, I; Gieraltowski, L

    2017-06-01

    Salmonella is a leading cause of bacterial foodborne illness. We report the collaborative investigative efforts of US and Canadian public health officials during the 2013-2014 international outbreak of multiple Salmonella serotype infections linked to sprouted chia seed powder. The investigation included open-ended interviews of ill persons, traceback, product testing, facility inspections, and trace forward. Ninety-four persons infected with outbreak strains from 16 states and four provinces were identified; 21% were hospitalized and none died. Fifty-four (96%) of 56 persons who consumed chia seed powder, reported 13 different brands that traced back to a single Canadian firm, distributed by four US and eight Canadian companies. Laboratory testing yielded outbreak strains from leftover and intact product. Contaminated product was recalled. Although chia seed powder is a novel outbreak vehicle, sprouted seeds are recognized as an important cause of foodborne illness; firms should follow available guidance to reduce the risk of bacterial contamination during sprouting.

  19. Effect of Brussels sprouts and inulin/rape seed cake on the sensory profile of pork M. longissimus dorsi.

    Science.gov (United States)

    Hansen, L L; Agerhem, H; Rosenvold, K; Jensen, M T

    2002-08-01

    The objective of the present study was to evaluate the influence of two diets containing either Brussels sprouts or inulin/rape seed cake, compared with a standard diet (control) for slaughter pigs on flavour and odour attributes and sensory profile of cooked pork. Three weeks prior to slaughter 24 female pigs were allocated to three diets: (1) a standard grower-finishing diet (control) for slaughter pigs containing barley, wheat and soy-bean meal, (2) the control diet containing 11 energy percent Brussels sprouts and (3) a diet containing 25% inulin and 55% rape seed cake. The odour and flavour of the cooked meat from inulin/rape seed cake-fed pigs differed significantly from the other two diets, showing reduced meat odour, increased pig and acrid odour, increased pig flavour, reduced fresh flavour and total impression. Meat from the Brussels sprouts-fed pigs deviated only slightly from the control-fed pigs.

  20. Acetic acid pretreatment improves the hardness of cooked potato slices.

    Science.gov (United States)

    Zhao, Wenlin; Shehzad, Hussain; Yan, Shoulei; Li, Jie; Wang, Qingzhang

    2017-08-01

    The effects of acetic acid pretreatment on the texture of cooked potato slices were investigated in this work. Potato slices were pretreated with acetic acid immersion (AAI), distilled water immersion (DWI), or no immersion (NI). Subsequently, the cell wall material of the pretreated samples was isolated and fractioned to evaluate changes in the monosaccharide content and molar mass (MM), and the hardness and microscopic structure of the potato slices in different pretreatments before and after cooking were determined. The results showed that the highest firmness was obtained with more intact structure of the cell wall for cooked potato slices with AAI pretreatment. Furthermore, the MM and sugar ratio demonstrated that the AAI pretreated potato slices contained a higher content of the small molecular polysaccharides of cell walls, especially in the hemicellulose fraction. This work may provide a reference for potato processing. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Survival of murine norovirus, Tulane virus, and hepatitis A virus on alfalfa seeds and sprouts during storage and germination.

    Science.gov (United States)

    Wang, Qing; Hirneisen, Kirsten A; Markland, Sarah M; Kniel, Kalmia E

    2013-11-01

    Human norovirus (huNoV) and hepatitis A virus (HAV) have been involved in several produce-associated outbreaks and identified as major food-borne viral etiologies. In this study, the survival of huNoV surrogates (murine norovirus [MNV] and Tulane virus [TV]) and HAV was investigated on alfalfa seeds during storage and postgermination. Alfalfa seeds were inoculated with MNV, TV, or HAV with titers of 6.46 ± 0.06 log PFU/g, 3.87 ± 0.38 log PFU/g, or 7.01 ± 0.07 log 50% tissue culture infectious doses (TCID50)/g, respectively. Inoculated seeds were stored for up to 50 days at 22°C and sampled during that storage period on days 0, 2, 5, 10, and 15. Following storage, virus presence was monitored over a 1-week germination period. Viruses remained infectious after 50 days, with titers of 1.61 ± 0.19 log PFU/g, 0.85 ± 0.21 log PFU/g, and 3.43 ± 0.21 log TCID50/g for MNV, TV, and HAV, respectively. HAV demonstrated greater persistence than MNV and TV, without a statistically significant reduction over 20 days (seed planting. Results revealed the persistence of viruses in seeds for a prolonged period of time, and perhaps of greater importance these data suggest the ease of which virus may transfer from seeds to sprouts and spent water during germination. These findings highlight the importance of sanitation and prevention procedures before and during germination.

  2. Mutagenicity studies on alcohol extracts from gamma-irradiated potatoes

    International Nuclear Information System (INIS)

    Shinozaki, Yoshiharu; Hogetsu, Daisuke; Okuyama, Norio; Manabe, Takashi; Sasagawa, Tatsuru.

    1981-01-01

    The preparation of alcohol extracts from gamma-irradiated potatoes of the ''Danshaku'' variety and their chemical aspects were studied. The final concentrate of alcohol extracts from potatoes showed pH values of 3 -- 4. The o-quinones or ''radiotoxins'' reported by Kuzin, et al. were not detected in the alcohol extracts from potatoes of this variety by high performance liquid chromatography (HPLC) analysis, paper chromatography and the model enzymatic experiment. (author)

  3. Combination processes for improving storage of irradiated potatoes

    International Nuclear Information System (INIS)

    Shirsat, S.G.; Thomas, P.; Nair, P.M.

    1990-01-01

    Various combined treatments of harvested potatoes to reduce microbial spoilage of irradiated potatoes in storage were studied. Treatments studied were irradiation in conjunction with hot water dip, cold or hot salicylic acid dip, dusting with salicylic acid powder, and sodium hypochlorite wash followed by storage in well aerated containers. Microbial spoilage in irradiated potatoes was found to be greatly reduced by storage at 10deg or 15degC in well aerated containers. (M.G.B.). 9 refs., 1 tab

  4. Biocontrol of the internalization of Salmonella enterica and Enterohaemorrhagic Escherichia coli in mung bean sprouts with an endophytic Bacillus subtilis.

    Science.gov (United States)

    Shen, Zhenyu; Mustapha, Azlin; Lin, Mengshi; Zheng, Guolu

    2017-06-05

    Internalization of Salmonella enterica and enterohaemorrhagic Escherichia coli (EHEC) in seed sprouts poses a health risk to consumers, and the conventional sanitization methods are not always effective to reduce this risk. This study initiated a biocontrol approach to limit the internalization using endophytic Bacillus subtilis strains, which were isolated from the inner tissue of mung bean seeds or lettuce stems. By using the deferred agar method, 12 strains of B. subtilis out of 94 putative Bacillus isolates displayed inhibitory activity against at least one of the pathogenic indicators, S. enterica Typhimurium ATCC 14028 and E. coli O157:H7 505B. Two B. subtilis isolates (LCA1 and M24) showed a broad inhibitory spectrum against multiple strains of S. enterica and EHEC, Staphylococcus aureus sp., Klebsiella pneumoniae ATCC 700603, and Listeria monocytogenes Scott A, while the laboratory B. subtilis strain 168 was only moderately inhibitory against L. monocytogenes. To facilitate the tracking of the three B. subtilis strains (LCA1, M24, and 168) in the mung bean sprouts, the three strains were genetically engineered to carry the chloramphenicol acetyltransferase (cat), generating the strains LCA1-cat, M24-cat, and 168-cat, respectively. Data of the study using the cat-tagged strains demonstrated that both the two vegetable-associated and the laboratory B. subtilis strains could internalize in mung bean sprouts during the sprouting, but the latter displayed about 1.2 lg CFU/g of seeds lower in internalization. Overall, the presence of the three B. subtilis strains could significantly reduce the internalization of S. enterica or EHEC cocktail in mung bean sprouts during the sprouting. Among them, LCA1 showed the greatest inhibition against the EHEC cocktails with a reduction of about 2.0lg CFU/g of seeds by the end of sprouting (day 5), while 168 had the smallest reduction at about 0.6lg CFU/g of seeds. In addition, the three strains demonstrated a similar

  5. Cryopreservation for preservation of potato genetic resources

    Science.gov (United States)

    Niino, Takao; Arizaga, Miriam Valle

    2015-01-01

    Cryopreservation is becoming a very important tool for the long-term storage of plant genetic resources and efficient cryopreservation protocols have been developed for a large number of plant species. Practical procedures, developed using in vitro tissue culture, can be a simple and reliable preservation option of potato genetic resources rather than maintaining by vegetative propagation in genebanks due their allogamous nature. Cryopreserved materials insure a long-term backup of field collections against loss of plant germplasm. Occurrence of genetic variation, in tissue culture cells during prolonged subcultures, can be avoided with suitable cryopreservation protocols that provide high regrowth, leading and facilitating a systematic and strategic cryo-banking of plant genetic resources. Cryopreservation protocols for potato reviewed here, can efficiently complement field and in vitro conservation, providing for preservation of genotypes difficult to preserve by other methods, wild types and other species decided as priority collections. PMID:25931979

  6. Ozone injury and infection of potato leaves by Botrytis cinerea

    Energy Technology Data Exchange (ETDEWEB)

    Manning, W.J.; Feder, W.A.; Perkins, I.; Glickman, M.

    1969-09-01

    Symptoms of ozone injury were observed on older leaves of potato cultivars Norland and Katahdin under experimental conditions. This symptom expression closely resembled flecks observed on potato leaves also blighted by Botrytis cinerea in the field. Inoculation of ozone-injured and noninjured potato leaves with B. cinerea showed that infection was more rapid and disease development more severe on ozone-injured leaves. Infection was frequently observed to originate in ozone-injured leaf areas. Ozone injury, under experimental conditions, appeared to increase the susceptibility of potato leaves to infection by B. cinerea. 6 references.

  7. THE STATISTICAL INDICATORS OF POTATO PRODUCED IN ROMANIA

    Directory of Open Access Journals (Sweden)

    Elena BULARCA

    2013-12-01

    Full Text Available In this study we have analyzed and interpreted the main statistical indicators of potato produced in Romania. First of all, we start by presenting some information about potatoes: origin and appearance, their importance and necessity in the life of people and animals. Then on the basis of the specific statistical indicators, it was interpreted the evolution of the cultivated area, the percentage of the main counties in the cultivated area with potatoes, the average yield per hectare, as well as the import and export of potatoes in a given period. Each indicator was analyzed and corresponding remarks and conclusions have been drawn.

  8. Naturally occurring allele diversity allows potato cultivation in northern latitudes.

    Science.gov (United States)

    Kloosterman, Bjorn; Abelenda, José A; Gomez, María del Mar Carretero; Oortwijn, Marian; de Boer, Jan M; Kowitwanich, Krissana; Horvath, Beatrix M; van Eck, Herman J; Smaczniak, Cezary; Prat, Salomé; Visser, Richard G F; Bachem, Christian W B

    2013-03-14

    Potato (Solanum tuberosum L.) originates from the Andes and evolved short-day-dependent tuber formation as a vegetative propagation strategy. Here we describe the identification of a central regulator underlying a major-effect quantitative trait locus for plant maturity and initiation of tuber development. We show that this gene belongs to the family of DOF (DNA-binding with one finger) transcription factors and regulates tuberization and plant life cycle length, by acting as a mediator between the circadian clock and the StSP6A mobile tuberization signal. We also show that natural allelic variants evade post-translational light regulation, allowing cultivation outside the geographical centre of origin of potato. Potato is a member of the Solanaceae family and is one of the world's most important food crops. This annual plant originates from the Andean regions of South America. Potato develops tubers from underground stems called stolons. Its equatorial origin makes potato essentially short-day dependent for tuberization and potato will not make tubers in the long-day conditions of spring and summer in the northern latitudes. When introduced in temperate zones, wild material will form tubers in the course of the autumnal shortening of day-length. Thus, one of the first selected traits in potato leading to a European potato type is likely to have been long-day acclimation for tuberization. Potato breeders can exploit the naturally occurring variation in tuberization onset and life cycle length, allowing varietal breeding for different latitudes, harvest times and markets.

  9. Potato production on private plots contaminated by radionuclides

    International Nuclear Information System (INIS)

    Bogdevitch, I.; Tarasiuk, S.

    2004-01-01

    Potatoes is one of the basis food products of man diet, especially in country area. Recent research has shown that 137 Cs content in potato tubers does not exceed the permissible level at the potato growing on radioactive contaminated land in Belarus. However up to 240-300 kg of potatoes is consumed per man during year and potato yield determines significant share of the internal dose of radiation. Moreover, extra potato yield sold on market is forming the essential part of the rural family budget. Therefore the reducing of 137 Cs content in potatoes and increasing of potato yield could be allowed to improve of quality life of rural inhabitants on contaminated areas. The application of protection measures on private plots are restricted by lack of the knowledge and financial deficit. The involvement of rural inhabitants in processes of self-rehabilitation and self-development could be a way to improve the quality life on radioactive contaminated territory There is strong motivation for inhabitants to increase the yield and to reduce the radionuclide concentration in potatoes. How to develop the conditions for the sustainable potato production by private producers on affected land? There are several factors to consider. Firstly, the rural inhabitants should be actively involved as initiators to improve their quality life. Secondly, the potato technology should be adopted to the local territory and tested by producers on radiological and economic efficiency. Thirdly, the common village initiatives should be developed for supplying by new varieties of seeds and fertilizers, selling of yield, consulting, crediting etc. The step-by-step solution of described strategy could allow improving and stabilizing the potato production by rural community. The training of rural people as an initial step was realized to transfer to the inhabitants the practical skills that can be used in their day-to-day life within framework of 'ETHOS-II' project. The experimental potato

  10. Potato growth in response to relative humidity

    Science.gov (United States)

    Wheeler, R. M.; Tibbitts, T. W.; Fitzpatrick, A. H.

    1989-01-01

    Potato plants (Solanum tuberosum L. cvs. Russet Burbank, Norland, and Denali) were grown for 56 days in controlled-environment rooms under continuous light at 20C and 50% or 85% RH. No significant differences in total plant dry weight were measured between the humidity treatments, but plants grown under 85% RH produced higher tuber yields. Leaf areas were greater under 50% RH and leaves tended to be larger and darker green than at 85% RH.

  11. Effects of gamma radiation on potato meristems

    International Nuclear Information System (INIS)

    Fernandez Gonzalez, J.; Garcia Collantes, M.A.

    1976-01-01

    The development of buds in potato tubers subjected to gamma radiation at doses of 3, 6, 8 and 12 Krad is studied at histological level. The irradiation was supplied at the beguining and end of the resting period, and the irradiated buds were observed at different stages of their development. Meristem's sensitivity depends on the state of activity involved at the moment of irradiation. Different parts of the meristem present different radiosensitivity, being the most radioresistant. (author) [es

  12. Stretch-induced intussuceptive and sprouting angiogenesis in the chick chorioallantoic membrane.

    Science.gov (United States)

    Belle, Janeil; Ysasi, Alexandra; Bennett, Robert D; Filipovic, Nenad; Nejad, Mohammad Imani; Trumper, David L; Ackermann, Maximilian; Wagner, Willi; Tsuda, Akira; Konerding, Moritz A; Mentzer, Steven J

    2014-09-01

    Vascular systems grow and remodel in response to not only metabolic needs, but also mechanical influences as well. Here, we investigated the influence of tissue-level mechanical forces on the patterning and structure of the chick chorioallantoic membrane (CAM) microcirculation. A dipole stretch field was applied to the CAM using custom computer-controlled servomotors. The topography of the stretch field was mapped using finite element models. After 3days of stretch, Sholl analysis of the CAM demonstrated a 7-fold increase in conducting vessel intersections within the stretch field (p0.05). In contrast, corrosion casting and SEM of the stretch field capillary meshwork demonstrated intense sprouting and intussusceptive angiogenesis. Both planar surface area (p<0.05) and pillar density (p<0.01) were significantly increased relative to control regions of the CAM. We conclude that a uniaxial stretch field stimulates the axial growth and realignment of conducting vessels as well as intussusceptive and sprouting angiogenesis within the gas exchange capillaries of the ex ovo CAM. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. Stretch-induced Intussuceptive and Sprouting Angiogenesis in the Chick Chorioallantoic Membrane

    Science.gov (United States)

    Belle, Janeil; Ysasi, Alexandra; Bennett, Robert; Filipovic, Nenad; Nejad, Mohammad Imani; Trumper, David L.; Ackermann, Max; Wagner, Willi; Tsuda, Akira; Konerding, Moritz A.; Mentzer, Steven J.

    2014-01-01

    Vascular systems grow and remodel in response to not only metabolic needs, but mechanical influences as well. Here, we investigated the influence of tissue-level mechanical forces on the patterning and structure of the chick chorioallantoic membrane (CAM) microcirculation. A dipole stretch field was applied to the CAM using custom computer-controlled servomotors. The topography of the stretch field was mapped using finite element models. After 3 days of stretch, Sholl analysis of the CAM demonstrated a 7-fold increase in conducting vessel intersections within the stretch field (p0.05). In contrast, corrosion casting and SEM of the stretch field capillary meshwork demonstrated intense sprouting and intussusceptive angiogenesis. Both planar surface area (p<0.05) and pillar density (p<0.01) were significantly increased relative to control regions of the CAM. We conclude that a uniaxial stretch field stimulates the axial growth and realignment of conducting vessels as well as intussusceptive and sprouting angiogenesis within the gas exchange capillaries of the ex ovo CAM. PMID:24984292

  14. Flavonoid synthesis in buckwheat ( Fagopyrum esculentum Moench ) sprout grown under pseudo-microgravity

    Science.gov (United States)

    Tomita-Yokotani, Kaori; Iwasawa, Hiroko; Hiraishi, Kanae; Sato, Seigo; Miyagawa, Teruo; Hashimoto, Hirofumi; Yamashita, Masamichi

    Habitation in outer space is one of our challenges. We are studying space agriculture to provide foods and oxygen for space habitats. However, careful assessment should be made on the effects of exotic environment on the endogenous production of biologically active substances in plants, which will be cultivated in space. We found that production of functional substances is affected by gravity in broccoli sprout (Brassica coleracea var. italica). The production of sulforaphane (4-methylsulfinybutyl isothiocyanate), in broccoli was slightly affected by gravity. Buckwheat is also known to produce several species of flavonoids, which act as an antioxidant, and enhance immunity of human. Such production of physiologically active substances, those agricultural species are accepted as good food materials. Buckwheat sprouts were cultivated for 4 days under the 3D-clinorotation. The amount of flavonoids, such as orientin, isoorientin, isovitexin, vitexin, rutin, produced by this treatment showed significant differences compared to those in the ground control. We examined effects of the gravity to the flavonoid synthesis pathways.

  15. Effect of harvest time on storage loss and sprouting in onion

    Directory of Open Access Journals (Sweden)

    T. SUOJALA

    2008-12-01

    Full Text Available Storability of onion is affected by timing of harvest. However, the optimal time for maximum yield and maximum storability do not necessarily coincide. This study aimed to determine the most suitable harvest time for obtaining a high bulb yield with high quality and storability. Storage experiments were conducted on onions produced in field experiments at a research field and on farms in four years. Results indicate that harvesting could be delayed to 100% maturity, or even longer, without a marked increase in storage loss. In rainy years, late harvest is likely to impair the quality. The incidence of sprouting in shelf life tests varied considerably between years. An early harvest before 50% maturity and a delayed harvest increased the risk of sprouting. It may be concluded that the harvesting of onions for long-term storage can be timed to take place between 50% maturity and even some weeks after complete maturity without a loss in storage quality. Therefore, it is possible to combine high yield and good storage quality.

  16. Study on iron availability from prepared soybean sprouts using an iron-deficient rat model.

    Science.gov (United States)

    Zielińska-Dawidziak, Magdalena; Hertig, Iwona; Piasecka-Kwiatkowska, Dorota; Staniek, Halina; Nowak, Krzysztof W; Twardowski, Tomasz

    2012-12-15

    During soya seeds germination in FeSO(4) solutions their phytoferritin content is multiplied. Prepared soybean sprouts have been proposed as a safe and easily available source of iron supplementation. The preparation was compared with FeSO(4) and ferritin isolates, using rats with induced iron deficiency anaemia. After the end of the 2-week supplementation experiment, it was observed that no statistically significant differences in haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration existed between those animals supplemented with sprouts enriched in ferritin, ferritin isolate and FeSO(4) and healthy animals forming the control group. Moreover, the examined preparation had a beneficial influence on the recreation of ferritin reserves in both the liver and the blood serum, and also did not induce negative alterations in general growth parameters of animals. Use of an easily obtainable ferritin iron source may be a profitable alternative in supplementation due to its wide availability and food preservative properties. Copyright © 2012 Elsevier Ltd. All rights reserved.

  17. Increased Sucrose in the Hypocotyls of Radish Sprouts Contributes to Nitrogen Deficiency-Induced Anthocyanin Accumulation.

    Science.gov (United States)

    Su, Nana; Wu, Qi; Cui, Jin

    2016-01-01

    Effects of nitrogen (N) deficiency and sucrose (Suc) addition on regulation of anthocyanin biosynthesis and their relationship were investigated in this study. Radish sprouts subjected to N deficiency had 50% higher anthocyanin accumulation than when grown in Hoagland solution (a nutrient medium with all macronutrients). The contents of endogenous soluble sugars (Suc, fructose, and glucose) in the hypocotyls were also markedly increased by N limitation, with Suc showing the highest increase. Inhibition of carbohydrate biosynthesis by addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) also eliminated N deficiency-induced anthocyanin accumulation. The latter was further supported by the expression of anthocyanin biosynthesis related genes and decreased activities of nitrate reductase in the presence of Suc. Together our results indicate that N deficiency-induced anthocyanin accumulation was, at least partly, dependent on the increase of the soluble sugar, especially Suc. This work is the first comprehensive study on relationship between N deficiency and sugar content on anthocyanin accumulation in the hypocotyls of radish sprouts.

  18. Study of selenocompounds from selenium-enriched culture of edible sprouts.

    Science.gov (United States)

    Funes-Collado, Virginia; Morell-Garcia, Albert; Rubio, Roser; López-Sánchez, José Fermín

    2013-12-15

    Selenium is recognised as an essential micronutrient for humans and animals. One of the main sources of selenocompounds in the human diet is vegetables. Therefore, this study deals with the Se species present in different edible sprouts grown in Se-enriched media. We grew alfalfa, lentil and soy in a hydroponic system amended with soluble salts, containing the same proportion of Se, in the form of Se(VI) and Se(IV). Total Se in the sprouts was determined by acidic digestion in a microwave system and by ICP/MS. Se speciation was carried out by enzymatic extraction (Protease XIV) and measured by LC-ICP/MS. The study shows that the Se content of plants depends on the content in the growth culture, and that part of the inorganic Se was biotransformed mainly into SeMet. These results contribute to our understanding of the uptake of inorganic Se and its biotransformation by edible plants. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. Potato cv. Romano reaction to primary and secondary infection with potato necrotic strain Y virus (PVYNTN

    Directory of Open Access Journals (Sweden)

    Drago Milošević

    2015-01-01

    Full Text Available Primary and secondary infections with PVYNTN were investigated on forty plants of the potato cv. Romano inoculated in a greenhouse in Serbia in 2012 and 2013. PVY isolates were collected from the potato growing region of Čačak and identified by ELISA and RT-PCR methods. The sequence of the Serbian isolate 3D (Acc. No. KJ946936 showed 100% match with seven PVY isolates deposited in GenBank and described as NTN. A significant difference was detected between PVYNTN symptoms exibited on leaves of the cv. Romano under primary and secondary infections. The findings are significant because they are based on symptoms observed, so that it is clear that there are two distinct types of infection: primary and secondary. Symptoms of primary and secondary infection were the same on potato tubers and had the form of necrotic rings.

  20. RESISTANCE OF POTATO GENOTYPES TO Meloidogyne javanica

    Directory of Open Access Journals (Sweden)

    JAQUELINE TAVARES SCHAFER

    2017-01-01

    Full Text Available The objective of this study was to evaluate the resistance of a group of clones and cultivars from the potato breeding program of Embrapa to Meloidogyne javanica . The experiment was conducted at Embrapa Temperate Climate, Pelotas / RS, under greenhouse conditions at temperatures of 25 ± 5°C. Individual potato plants of different genotypes [BRSIPR Bel, BRS F63 (Camila, CL02 - 05, F23 - 11 - 06, F32 - 02 - 06, F38 - 03 - 07, F189 - 09 - 06, F23 - 24 - 06 and F22 - 01 - 08], kept in pots with sterilized soil were inoculated with 5,000 eggs and J2 of M. javanica , using six replicates per treatment. The susceptible control, potato cultivar BRS Ana was inoculated with the same level of inoculum. Fifty - five days after inoculation, the number of galls on the roots of each plant was determined as well as the number of protuberances caused by nematodes in the different treatments. Then, the roots of each plant were processed for counting the number of eggs and J2, as well as determining the nematode reproduction factor (FR: final population / initial population. All genotypes were susceptible (FR> 1.00 to M. javanica . However, there were different levels of susceptibility among the cultivars tested.

  1. Studies for Somatic Embryogenesis in Sweet Potato

    Science.gov (United States)

    Bennett, J. Rasheed; Prakash, C. S.

    1997-01-01

    The purpose of this study was to improve the somatic embryo (SE) system for plant production of sweet potato (Ipomoea batatas L(Lam)). Explants isolated from SE-derived sweet potato plants were compared with control (non SE-derived) plants for their competency for SE production. Leaf explants were cultured on Murashige-Skoog (MS) medium with 2,4-dichlorophenoxy acetic acid (0.2 mg/L) and 6-benzylaminopurine (2.5 mg/L) for 2 weeks in darkness and transferred to MS medium with abscisic acid (2.5 mg/L). Explants isolated from those plants developed through somatic embryogenesis produced new somatic embryos rapidly and in higher frequency than those isolated from control plants They also appeared to grow faster in tissue culture than the control plants. Current studies in the laboratory are examining whether plants derived from a cyclical embryogenesis system (five cycles) would have any further positive impact on the rapidity and frequency of somatic embryo development. More detailed studies using electron microscopy are expected to show the point of origin of the embryos and to allow determination of their quality throughout the cyclical process. This study may facilitate improved plant micropropagation, gene transfer and germplasm conservation in sweet potato.

  2. The unlucky potato: Study of consumer risk perception toward a GM-potato using mental models

    DEFF Research Database (Denmark)

    Hagemann, Kit; Scholderer, Joachim

    The second generation of GMO aims at providing consumers with food that has a clear health benefit such as rice with enhanced nutrient levels or potatoes with a lower level of natural toxin. The new generation with health enhancing attributes is predicted to reduce consumer skepticism toward use ...... of GMO in food production, but a new and ongoing study on consumers' risk perception in regard to a GMO potato reveals that other barriers for acceptance of GMO food exist, barriers such as personal eating history....

  3. The carry-through of residues of maleic hydrazide from treated potatoes, following manufacture into potato crisps and 'jacket' potato crisps.

    Science.gov (United States)

    Lewis, D J; Thorpe, S A; Wilkinson, K; Reynolds, S L

    1998-07-01

    Potatoes, which had been treated 'in the field' with a commercial formulation of maleic hydrazide, were processed into potato crisps and jacket potato crisps on a factory production line using standard manufacturing conditions. Samples were taken at strategic points throughout the process and analysed to determine the degree of carry-through of residues. Results demonstrated that ca 56% of the maleic hydrazide residue in a potato could be carried through into the potato crisps, irrespective of which type of crisp was being manufactured. Results from a similarly constructed study investigating the fate of pesticides applied post-harvest showed that carry-through was less than 10%. This difference is explained in terms of the different modes of action of the two classes of pesticides being investigated. It is known that, as maleic hydrazide is a systemic pesticide, it will be located within the flesh of the potato tuber and is therefore likely to be protected from the various stages of the crisping process. However, the post-harvest non-systemic pesticides are applied to the exterior surface of the tuber and are therefore not likely to be protected in the same way. The results also showed that, due to the concentration effect caused by the loss of moisture during crisp manufacture, the levels of maleic hydrazide residues in crisps (on a mg/kg product basis) were approximately twice those measured in the original potatoes.

  4. Phylogenetic relationships of closely related potyviruses infecting sweet potato determined by genomic characterization of Sweet potato virus G and Sweet potato virus 2.

    Science.gov (United States)

    Li, Fan; Xu, Donglin; Abad, Jorge; Li, Ruhui

    2012-08-01

    Complete nucleotide sequences of Sweet potato virus G (SPVG) and Sweet potato virus 2 (SPV2) were determined to be 10,800 and 10,731 nucleotides, respectively, excluding the 3'-poly(A) tail. Their genomic organizations are typical of potyviruses, encoding a polyprotein which is likely cleaved into 10 mature proteins by three viral proteinases. Conserved motifs of orthologous proteins of viruses in the genus Potyvirus are found in corresponding positions of both viruses. Pairwise comparisons of individual protein sequences of the two viruses with those of 78 other potyviruses show that P1 protein and coat protein (CP) of both viruses are significantly large, with the SPVG CP as the largest among the all the known species of the genus Potyvirus. The extended N-terminal region of the P1 protein is conserved in the potyviruses and ipomovirus infecting sweet potato. A novel ORF, PISPO, is identified within the P1 region of SPVG, SPV2, Sweet potato feathery mottle virus (SPFMV), and Sweet potato virus C (SPVC). The C-terminal half of CP is highly conserved among SPFMV, SPVC, SPVG, SPV2, and Sweet potato virus-Zimbabwe. Phylogenetic analysis based on the deduced CP amino acid sequences supports the view that these five viruses are grouped together in a SPFMV lineage. The analysis also reveals that Sweet potato virus Y and Ipomoea vein mosaic virus are grouped with SPV2 as one species, and these two viruses should be consolidated with SPV2.

  5. Use of gamma radiation as a form of preservation of sweet potatoes: Phase 2: Final report for period January 1, 1986-December 31, 1986

    International Nuclear Information System (INIS)

    Tolbert, M.E.M.; Loretan, P.A.

    1987-04-01

    Effects of gamma radiation and storage time on storage roots of 'Georgia Jet' and 'Jewel' sweet potatoes and on the sweetpotato weevil Cylas formicarius elegantulus (Summers) were studied. The storage roots were irradiated: immediately after harvest and before curing immediately after curing, or after curing plus three months of storage. Storage was at 14 to 16 0 C with 85 to 90% relative humidity. Sweet potatoes were examined at different stages of storage over nine months for sprouting, storage rot development, flesh color, texture, moisture, carbohydrates, starch granules - cell ultrastructure, ascorbic acid, enzyme activity, peroxidase and polyphenol oxidase, protein and organoleptic properties. It was found that sweet potatoes should never be irradiated prior to curing since increased rotting results. No significant difference was found in storage rot development between control and irradiated after curing at doses up to 0.20 kGy. Nutritive components were in most cases not significantly affected by radiation at the four doses studies. Nutritive qualities of moisture, texture, starch, ascorbic acid, sugar, color and enzymes all changed to one extent or another with increased storage time. Irradiation speeds up the process of cell wall collapse and accumulation of cytoplasmic debris, but this also occurs later in the control. Isoelectric focusing results demonstrate that radiation effects on proteins are very small up to a dose of 0.20 kGy. HPLC results indicated that irradiation of the roots caused accumulation of new proteins while impairing the formation of others. Fecundity of the sweetpotato weevil was reduced at 15 0 C compared to 28 0 C

  6. Absorção de nutrientes pela batatinha Nutritional studies with the potato plant

    Directory of Open Access Journals (Sweden)

    H. Gargantini

    1963-01-01

    Full Text Available A marcha da absorção dos elementos nutritivos essenciais pela batatinha (Solanum tuberosum L. foi estudada em plantas cultivadas em vasos de barro, interna, mente vidrados, e com capacidade para 10 quilos de terra. Em cada 10 dias, durante todo o ciclo vegetativo, eram colhidas plantas e analisados os teores de N, P, K, Ca, Mg e S. Tôdas as plantas receberam os mesmos cuidados em tratos culturais, fitossanitários e água. Propiciaram-se, ainda, iguais condições de fertilidade, dando-se a todos os vasos adubação completa, inclusive com os micronutrientes necessários ao bom desenvolvimento e produção da batata. Os resultados obtidos mostram que a batata absorve em grande quantidade o nitrogênio e o potássio, sendo o último em maior proporção. Dos outros elementos estudados, o fósforo, o cálcio, o magnésio e enxôfre, são absorvidos em pequenas quantidades, não ultrapassando nenhum dêles 16 kg/ha. Os dados mostram ainda que as quantidades totais necessárias de nitrogênio, potássio, magnésio e enxôfre são absorvidas pela cultura, até completar 50 dias após a germinação, enquanto o fósforo e o cálcio são requeridos desde o inicio até o final do ciclo vegetativo da planta.Nutritional studies were carried out with potted potato plants, aiming at determining the absorption rate and uptake of essential nutrients in relation to age of the crop. The experimental plants were grown in pots containing 10 kg of soil. Cultural practices, water, and fertility conditions (including addition of minor elements were uniform for all pots. Samples composed of an adequate number of plants (according to age were harvested at ten-day intervals during the entire vegetative cycle of the crop and analysed for N, P, K, Ca, Mg, and S. The results obtained indicated that the potato plant absorbs a large amount of nitrogen, followed by potassium. Phosphorus, calcium, magnesium, and sulfur are absorbed in small quantities, not more than 16 kg

  7. The effect of injury on whole-plant senescence: an experiment with two root-sprouting Barbarea species

    Czech Academy of Sciences Publication Activity Database

    Martínková, Jana; Šmilauer, P.; Mihulka, S.; Latzel, Vít; Klimešová, Jitka

    2016-01-01

    Roč. 117, č. 4 (2016), s. 667-679 ISSN 0305-7364 R&D Projects: GA ČR GB14-36079G Institutional support: RVO:67985939 Keywords : injury * senescence * root sprouting Subject RIV: EH - Ecology, Behaviour Impact factor: 4.041, year: 2016

  8. UV-B-induced anthocyanin accumulation in hypocotyls of radish sprouts continues in the dark after irradiation.

    Science.gov (United States)

    Su, Nana; Lu, Yanwu; Wu, Qi; Liu, Yuanyuan; Xia, Yan; Xia, Kai; Cui, Jin

    2016-02-01

    Raphanus sativus L. cv. Yanghua sprouts are rich in health-promoting anthocyanins; thus hypocotyls show a red color under light. In this study, effects of UV-B irradiation at 5 W m(-2) on anthocyanin biosynthesis in the hypocotyls of radish sprouts were investigated. Anthocyanins began to accumulate rapidly from 24 h irradiation and increased continuously until 48 h, showing a similar pattern to phenylalanine ammonia lyase (PAL) activity, with a correlation coefficient of 0.804. The expression of DFR and ANS paralleled the upward trend in anthocyanin accumulation, while CHS, CHI and F3H were upregulated before accumulation. When sprouts were moved into the dark from UV-B, the anthocyanin accumulation did not stop immediately. By contrast, anthocyanin accumulated continuously for more than 12 h in the dark, which was further supported by the significantly higher PAL activity monitored at 24 h after irradiation. Similarly, the transcript levels of anthocyanin biosynthesis-related genes were much higher over 6 h after 12 h UV-B irradiation. UV-B-induced anthocyanin accumulation continues in the dark after irradiation, which was supported by unfading PAL activity and high levels of biosynthesis-related genes. This will provide evidence to produce high-quality sprouts with more anthocyanins but less energy wastage in practice. © 2015 Society of Chemical Industry.

  9. Survival of Salmonella enterica on soybean sprouts following treatments with gaseous chlorine dioxide and biocontrol Pseudomonas bacteria

    Science.gov (United States)

    Control of Salmonella enterica on sprouts and minimally processed, ready-to-eat fruits and vegetables is important for food and consumer safety. The aim of this research was to assess the effects of gaseous chlorine dioxide(ClO2)and biocontrol microorganisms (Pseudomonas chlororaphis and P. fluoresc...

  10. Survival of Salmonella Typhimurium on soybean sprouts after treatment with gaseous chlorine dioxide and biocontrol Pseudomonas bacteria

    Science.gov (United States)

    Control of Salmonella Typhimurium on sprouts and minimally processed produce is crucial for food and consumer safety. The aim of this research was to assess natural microflora populations on soybean and evaluate the effects of gaseous chlorine dioxide (ClO2) and biocontrol Pseudomonas on the surviva...

  11. Enrichment of Gamma-Aminobutyric Acid in Bean Sprouts: Exploring Biosynthesis of Plant Metabolite Using Common Household Reagents

    Science.gov (United States)

    Rojanarata, Theerasak; Plianwong, Samarwadee; Opanasopit, Praneet; Ngawhirunpat, Tanasait

    2018-01-01

    The enrichment of plant foods with gamma-aminobutyric acid (GABA) is currently an interesting issue in the field of nutraceuticals and can be used as an experiment for upper-division undergraduate students. Here, an interdisciplinary hands-on experiment to produce GABA-enriched mung bean sprouts using common household reagents is described. Based…

  12. Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by HSCCC.

    Science.gov (United States)

    Lv, Qiaoying; Yang, Yi; Zhao, Yongxin; Gu, Dongyu; He, Dajun; Yili, Abulimiti; Ma, Qingling; Cheng, Zhen; Gao, Yanhua; Aisa, Haji Akber; Ito, Yoichiro

    2009-01-01

    Chickpea is known as a plant that is rich in protein, carbohydrates, and nutrition, and its seeds and sprouts have been processed into various health foods. In the present study, four isoflavones were purified from the seeds and sprouts of chickpea by high speed countercurrent chromatography (HSCCC) using two biphasic solvent systems composed of n-hexane-ethyl acetate-methanol-water (5:5:5:5, v/v) and ethyl acetate-water (1:1 v/v). The results indicated that 14.2 mg of formononetin, 15.7 mg of biochanin A, 9.1 mg of ononin, 11.3 mg of biochanin A-7-O-β-D-glucoside were obtained from 150 mg of sprout extracts with the purity of 92.26%, 95.86%, 95.32%, and 96.56%, respectively. Compared with the sprouts, separation of seed extracts yielded less amounts of biochanin A-7-O-β-D-glucoside and biochanin A with lower purity. The results indicate that four main isoflavones in chickpea, i.e., isoflavones, formononetin, biochanin A, ononin, and biochanin A-7-O-β-D-glucoside, are substantially increased by biosynthesis during the seed germination.

  13. Single-tree harvesting reduces survival and growth of oak stump sprouts in the Missouri Ozark Highlands

    Science.gov (United States)

    Daniel C. Dey; Randy G. Jensen; Michael J. Wallendorf

    2008-01-01

    Regeneration and recruitment into the overstory is critical to the success of using uneven-aged systems to sustain oak forests. We evaluated survival and growth of white oak (Quercus alba L.), black oak (Q. velutina Lam.), and scarlet oak (Q. coccinea Muenchh.) stump sprouts 10 years after harvesting Ozark...

  14. Late Blight of Potato (Phytophthora infestans I: Fungicides Application and Associated Challenges

    Directory of Open Access Journals (Sweden)

    Abdul Majeed

    2017-03-01

    Full Text Available Potato (Solanum tuberosum L. has been remained an important agricultural crop in resolving global food issues through decades. The crop has experienced enormous growth in terms of production throughout the world in recent decades because of improvement in agricultural mechanization, fertilizers application and irrigation practices. Nevertheless, a significant proportion of this valuable crop is still vulnerable to losses due to prevalence of different viral, bacterial, fungal and nematodes infestations. Late blight, caused by Phytophthora infestans (Mont. de Bary, is one of the most threatening pathogenic diseases which not only results in direct crop losses but also cause farmers to embrace huge monetary expenses for disease control and preventive measures. The disease is well known for notorious ‘Irish Famine’ which resulted in drop of Irish population by more than 20% as result of hunger and potato starvation. Globally, annual losses of crop and money spend on fungicides for late blight control exceeds one trillion US dollars. This paper reviews the significance of late blight of potato and controlling strategies adopted for minimizing yield losses incurred by this disease by the use of synthetic fungicides. Advantages and disadvantages of fungicides application are discussed.

  15. Neonicotinoids as seed potato treatments to control wireworms

    NARCIS (Netherlands)

    Huiting, H.F.; Ester, A.

    2009-01-01

    A series of field trials were carried out from 2000 to 2003. Neonicotinoid insecticides applied as seed potato treatments at planting were tested to control wireworms in potato crops. Compounds were applied as drench or spray. Neonicotinoids tested were imidacloprid at rates of 35, 70, 88, and 175 g

  16. Utilization of sweet potato starches and flours as composites with ...

    African Journals Online (AJOL)

    :20, 70:30, 60:40 and 50:50. Also, blends of wheat and sweet potato starch were developed in the ratios 80:20, 70:30, 60:40 and 50:50. Whole sweet potato flour and starch were also included where 100% wheat flour was used as control or ...

  17. In vitro methods for mutation induction in potato ( Solanum ...

    African Journals Online (AJOL)

    Potato (Solanum tuberosum L.) is an important vegetable and staple crop worldwide and mainly propagated vegetatively. Breeding of potato is problematic and therefore induced mutation is an attractive means of improving the crop. In vitro culture systems, and especially the production of microtubers, are ideal for such ...

  18. Determinants of Sweet Potato Value Addition among Smallholder

    African Journals Online (AJOL)

    sweet potato harvested significantly increased farmers' decision to add value by 0.494 units and 0.003 units respectively. Furthermore ... Key words: Sweet potato, value addition, Heckman two-stage model, Kwara State, Nigeria. INTRODUCTION ... governments to focus on the whole value chain from production to markets.

  19. Occurrence of Pospiviroid in potato, tomato and some ornamental ...

    African Journals Online (AJOL)

    USER

    2010-05-03

    May 3, 2010 ... Reverse transcription-polymerase chain reaction (RT-. PCR) detection of two Pospiviroids from potato tubers and chrysanthemum. Lanes 1, 2, 3, 4, 5 and 6 PSTVd isolates; lane. 7, healthy potato tuber (negative control); lane 8, positive control for PSTVd; lane 8, healthy Chrysanthemum and lane 10, CSVd.

  20. ​Improving potato production for increased food security of ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Three or four highly nutritious potato clones with resistance to late blight disease; Agronomic and nutritional characterization of 202 potato genotypes; A forecasting ... IDRC and the São Paulo Research Foundation (FAPESP) signed a scientific and technological cooperation agreement to support joint research projects in ...

  1. Colonization of wild potato plants by Streptomyces scabies

    Science.gov (United States)

    The bacterial pathogen Streptomyces scabies produces lesions on potato tubers, reducing their marketability and profitability. M6 and 524-8 are two closely related inbred diploid lines of the wild potato species Solanum chacoense. After testing in both field and greenhouse assays, it was found that ...

  2. Gender and Relative Economic Efficiency in Sweet Potato Farms of ...

    African Journals Online (AJOL)

    This study employed the stochastic frontier cost function to measure the level of economic efficiency and its determinants in small-scale sweet potato production in Imo State, Nigeria on gender basis. A multi-stage random sampling technique was used to select 120 sweet potato farmers (64 females and 56 males) in the ...

  3. Resource Use Efficiency in Sweet Potato Production in Kwara State ...

    African Journals Online (AJOL)

    This paper examines resource use efficiency in sweet potato production in Offa and Oyun local government areas of Kwara State of Nigeria. Primary data were collected from one hundred sweet potato farmers who were selected from the two local government areas during the 2003/2004 farming season. The data was ...

  4. Profitability of sweet potato production in derived savannah zone of ...

    African Journals Online (AJOL)

    This study examined profitability of sweet potato production in Odeda Local Government Area, Ogun State, Nigeria. The study was based on primary data collected from 82 sweet potato farmers through multistage sampling technique; analysed using descriptive statistics and budgetary techniques. The result revealed that ...

  5. Quality evaluation of 'gari' produced from cassava and sweet potato ...

    African Journals Online (AJOL)

    The quality evaluation of gari produced from mixes of cassava (Manihot esculenta, Crantz) and Sweet potato (Ipomoea batatas) tubers were investigated and reported. This is done to allow for the establishment of the best mixes of cassava and potato tubers to be adopted in the composite gari produced. Gari was produced ...

  6. Quality assessment of flour and bread from sweet potato wheat ...

    African Journals Online (AJOL)

    This study was to assess the quality of the flour and bread produced from sweet potato wheat composite flour blends. Matured and freshly harvested sweet potato (Ipomea batatas L.) was obtained from a local market in Akure, Nigeria. The tubers were thoroughly washed, peeled, washed again, drained, chipped, oven dried, ...

  7. Functional and pasting properties of cassava and sweet potato ...

    African Journals Online (AJOL)

    The functional and pasting properties of cassava starch and sweet potato starch mixtures at different ratios were investigated. Starches from four different cassava genotypes ('Adehye', AFS048, 'Bankye Botan' and OFF146) and one local sweet potato were used for the study. The swelling volume and swelling power of ...

  8. ( Coturnix coturnix japonica ) fed processed sweet potato ( Ipomea ...

    African Journals Online (AJOL)

    A six–week feeding trial was carried out to investigate the effect of processing of sweet potato tuber on growth parameters and carcass values of Japanese quails. Five isonitrogenous (25%CP) diets were compounded. The control diet (A) had zero sweet potato tuber meal. The other four diets (B, C, D and E) contained ...

  9. Effects of sweet potato meal on performance and carcass ...

    African Journals Online (AJOL)

    Three hundred and five (305) five weeks old broilers (Anak strain) were used in a four-week experiment to determine the effect of dietary substitution of processed sweet potato meal for maize grain on the carcass quality of broilers at the finisher phase. Graded levels of processed sweet potato meal (0, 12.5, 25, 37.5 and ...

  10. 76 FR 31787 - United States Standards for Grades of Potatoes

    Science.gov (United States)

    2011-06-02

    ... edible or marketing quality, or the internal or external appearance of the potato, or any external defect... DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 51 [Doc. AMS-FV-08-0023] United States Standards for Grades of Potatoes AGENCY: Agricultural Marketing Service, USDA. ACTION...

  11. Healthier, more nutritious potatoes improve food security in Colombia

    International Development Research Centre (IDRC) Digital Library (Canada)

    2016-04-26

    Apr 26, 2016 ... The potato is one of Colombia's most important staple foods and a significant source of low-cost calories for families. However, yellow potato varieties grown in the Nariño region in southern Colombia are low-yielding and highly susceptible to late blight disease. In response to this problem, an innovative ...

  12. Diversity and evolution of potato mop-top virus

    DEFF Research Database (Denmark)

    Beuch, Ulrike; Beuch, Sofia; Åkerblom, Jonas

    2015-01-01

    Nearly complete sequences of RNA-CP and 3′-proximal RNA-TGB were determined for 43 samples of potato mop-top virus (PMTV) originating from potato tubers and field soil from Sweden, Denmark and the USA. The results showed limited diversity and no strict geographical grouping, suggesting only a few...

  13. Using Sweet Potato Amylase Extracts for the Determination of Starch ...

    African Journals Online (AJOL)

    A study was carried out to assess the possibility of quantitative determination of starch in starchy foodstuffs using crude amylase extracts from Ugandan sweet potato cultivars. Amylolytic activity in 18 sweet potato cultivars grown at Namulonge was evaluated and there was a significant variation of activity among cultivars ...

  14. Consumer perceptions and demand for biofortified sweet potato ...

    African Journals Online (AJOL)

    Certain varieties of sweet potato, especially orange-fleshed, are being promoted as part of the strategy to combat vitamin A deficiency in children and pregnant mothers. However, the consumption of sweet potato is more widespread in rural households where it is mainly boiled or eaten raw. The lack of value addition ...

  15. Integrated nutrient management for orange-fleshed sweet potato in ...

    African Journals Online (AJOL)

    In the rainforest of south eastern Nigeria, new varieties of orange-fleshed sweet potatoes (Ipomea batatas Lam) have been introduced but appropriate soil nutrient management for these cultivars is lacking. The present study evaluated the response of two varieties of orange-fleshed sweet potatoes (Umuspo 1 and Umuspo ...

  16. Microbial biodegradable potato starch based low density polyethylene

    African Journals Online (AJOL)

    Plastic materials remain in the nature for decades. Slow degradation of plastics in the environment caused a public trend to biodegradable polymers. The aim of this research was to produce the microbial biodegradable low density polyethylene with potato starch. Degradation of potato starch based low density polyethylene ...

  17. Vitamin B9 is Higher in Developmentally Younger Potato Tubers

    Science.gov (United States)

    BACKGROUND: Folate (Vitamin B9) deficiency is a global nutritional problem and is problematic even in the developed world. Several studies have shown that because of their large consumption potatoes are an important source of dietary folate. New or baby potatoes constitute a valuable niche market th...

  18. introduction aerobic mesophilic bacteria associated with irish potato

    African Journals Online (AJOL)

    faecalis showed inhibitory activity due to the effect of organic acid against Erwinia sp. and Bacillus sp. (A ). The. 1 results indicated the high prevalence of antibiotic resistant strains associated with the spoilage of Irish potato. Key words: Irish Potato, Aerobic Mesophilic Bacteria, Antimicrobial Agents, Lactic Acid Bacteria,.

  19. A Collaborative Breeding Strategy for Organic Potatoes in the Netherlands

    NARCIS (Netherlands)

    Lammerts Van Bueren, E.

    2010-01-01

    Average potato yields in Dutch organic farming are rather low and variable, due to the disease known as late blight (Phytophthora infestans). Potato breeding companies, organic farmers and breeding scientists from the Louis Bolk Institute and Wageningen University, have joined forces in an umbrella

  20. Performance Stability Analysis of Potato Varieties under Rainfed ...

    African Journals Online (AJOL)

    In Ethiopia, farmers cultivate potato both in the rainy season under rainfed condition and the dry season using irrigation. Despite the variation in climatic condition and production constraints between the two systems, farmers grow the same variety both in rainfed and irrigated potato production systems. Moreover ...

  1. Recent Advances in Solanum Potato Improvement In Uganda

    African Journals Online (AJOL)

    The potato has also attained a status of one of the most imponant food crops in Uganda, par- ticularly in the highlands with subsistence agri- cultural production. Because of its short dura- tion, most farmers cultivate small plots of pota- toes in rotation with other crops. Current potato production for 1988 is reported to be ...

  2. Ecology and control of Dickeya spp. in potato

    NARCIS (Netherlands)

    Czajkowski, Robert

    2011-01-01

    Potato blackleg caused by pectinolytic Pectobacterium and Dickeya species is a bacterial disease creating serious economic losses in (seed)potato production worldwide. Effective management to control blackleg is absent and validated, cost-effective detection protocols for blackleg bacteria do not

  3. Occurrence of Pospiviroid in potato, tomato and some ornamental ...

    African Journals Online (AJOL)

    Of these samples, potato tubers were collected from markets, leaf samples from potato fields (Erzurum), tomato samples from field (Tokat, Amasya, Balýkesir and Bursa) and greenhouses (Antalya and Mula), leaves and vines of ornamental plants from greenhouses (Yalova) and florists (Erzurum) and recreation areas ...

  4. Challenges and opportunities for quality seed potato availability and ...

    African Journals Online (AJOL)

    ACSS

    growing crops in nutrient medium. The growing of potato plants in aeroponics system is considered as safe and ecologically friendly for producing natural, healthy plants and crops. This technology has advantages over the other systems or techniques, including conventional seed potato production, hydroponics and plant.

  5. Production system and varietal effects on potato production in Jos ...

    African Journals Online (AJOL)

    To address the problem of potato production in Nigeria, the Potato Research Programme of National Root Crops Research Institute, Umudike was established in Kuru, Jos in 1976. Data was collected with the aid of a structured questionnaire from 182 respondents involved in the the study. The aims of this study were to test ...

  6. Phenolic Compounds in the Potato and Its Byproducts: An Overview.

    Science.gov (United States)

    Akyol, Hazal; Riciputi, Ylenia; Capanoglu, Esra; Caboni, Maria Fiorenza; Verardo, Vito

    2016-05-27

    The potato (Solanum tuberosum L.) is a tuber that is largely used for food and is a source of different bioactive compounds such as starch, dietary fiber, amino acids, minerals, vitamins, and phenolic compounds. Phenolic compounds are synthetized by the potato plant as a protection response from bacteria, fungi, viruses, and insects. Several works showed that these potato compounds exhibited health-promoting effects in humans. However, the use of the potato in the food industry submits this vegetable to different processes that can alter the phenolic content. Moreover, many of these compounds with high bioactivity are located in the potato's skin, and so are eliminated as waste. In this review the most recent articles dealing with phenolic compounds in the potato and potato byproducts, along with the effects of harvesting, post-harvest, and technological processes, have been reviewed. Briefly, the phenolic composition, main extraction, and determination methods have been described. In addition, the "alternative" food uses and healthy properties of potato phenolic compounds have been addressed.

  7. The Potato Association of America: Working for you since 1913

    Science.gov (United States)

    The history and contributions of the Potato Association of America (PAA) to the advancement of the potato industry will be given with specific examples of how the industry has benefitted from the research and extension activities of the PAA. The primary purpose of the poster being to further familia...

  8. Evaluation of potato ( Solanum tuberosum L.) genotypes for yeild ...

    African Journals Online (AJOL)

    ... 20°C. The production of potato at lowland areas demands to develop heat tolerant cultivars adaptable specific to high temperatures. Therefore, this research was conducted with the objectives of evaluating the yield and tuber quality related traits of 26 potato genotypes and estimating the genetic diversity under heat stress ...

  9. The synthesis of 16-dehydropregnenolone acetate (DPA) from potato glycoalkaloids

    NARCIS (Netherlands)

    Vronen, P.J.E.; Koval, N.; Groot, de Æ.

    2004-01-01

    The use of solanidine as starting material for the synthesis of steroid hormones was strongly stimulated by the possibility to isolate large amounts ( ton scale) of potato glycoalkaloids from a waste stream of the potato starch production. A procedure is available to isolate these glycoalkaloids

  10. Potential of aeroponics system in the production of quality potato ...

    African Journals Online (AJOL)

    Plants once cleaned through meristem culture and induction of tuberization under aeroponics system, produce high quality potato seed tubers rapidly that are free from contamination of pathogens. Further multiplication of potato seed tubers under aeroponics also compliments tissue culture (micropropagation), as it clones ...

  11. Molecular characterization of capsid protein gene of potato virus X ...

    African Journals Online (AJOL)

    sami siraj

    2012-09-13

    Sep 13, 2012 ... 2Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan. 3Institute of Agricultural ... first report on the molecular characterization of full length PVX coat protein sequence infecting potato from Pakistan. ... sensitive and reliable detection methods (Salazar, 1994). Potato virus X ...

  12. SWEET POTATO CULTURE – PROMISING TREND OF RUSSIAN VEGETABLE GROWING

    OpenAIRE

    V. B. Podlesny

    2014-01-01

    Results of research of possibility of introduction of a new for the Russian Federation tuberous crop culture, sweet potato, are presented. The influence of planting dates on the yield of this culture was studied. According to the field experiment, the high yield of sweet potato tuber and resistance to diseases and pests were revealed.

  13. Potato Bean: Potential Forage/Dietary Supplement for Small Ruminants

    Science.gov (United States)

    Potato bean (Apios americana Medikus) is a nitrogen-fixing, perennial, leguminous vine indigenous to the eastern half of the United States. This vine climbs on plants and objects making its foliage accessible to browsing animals. We have observed deer eating potato bean foliage. Both deer and goa...

  14. Whole sales market for fresh potatoes in Finland

    Directory of Open Access Journals (Sweden)

    K. JALONOJA

    2008-12-01

    Full Text Available The study estimates a conditional mean and conditional variance model for producer prices of fresh potatoes. The results suggest that potato price movements are volatile exhibiting a symmetric and non-stationary process. Prices respond symmetrically to exogenous shocks and the shocks are, thereafter, predicted to prevail in prices to the end of the marketing year. The persistency of the price shocks makes potato price movements unpredictable and, therefore, increases price risks of holding potato inventories. The estimates indicate elastic price response with respect to annual potato yield shocks. A ten percent yield increase is predicted to decrease prices by 20%. The information on inventory levels is included in prices and this information is not increased by surveying the inventory levels. Because of the elastic price response, the largest risk for a farmer is an exceptionally large total yield of potatoes. Information on the aggregate potato yield, which arrives during the growing season, will be quickly incorporated in prices. Therefore, pre-harvest hedging strategies are more efficient than after-harvest hedging strategies in managing potato price risks.

  15. Potato carbohydrates: what’s in the middle counts

    Science.gov (United States)

    Potato is one of the world’s most important foods. Accumulation of starch in potato tubers depends on photosynthesis. Maintenance of 100% light capture per unit land area during the target growing season maximizes yields. Warm temperatures increase shoot and tuber respiration rates and depress tuber...

  16. Genetic diversity assessment of farmers' and improved potato ...

    African Journals Online (AJOL)

    Biniam M

    2016-08-31

    Aug 31, 2016 ... Sixty three potato clones (51 farmers' and 12 varieties) from Eritrea, 18 and 12 varieties from Kenya and. Rwanda, respectively were ... The crop plays a significant role in human nutrition worldwide, where more than 320 ..... to the fact that potato seed tubers are imported to the country from different sources ...

  17. Genetic analysis of root-knot nematode resistance in potato

    NARCIS (Netherlands)

    Draaistra, J.

    2006-01-01

    The development of potato varieties with resistance towards the potato cyst nematode, allowed a dramatic decrease of the use of nematicides. Subsequently the population of the free living nematodes and the root-knot nematodes ( Meloidogyne spp.) has increased. Among the root-knot nematodes, three

  18. Impact of technological change on output of potato production in ...

    African Journals Online (AJOL)

    This study assessed the impact of technological change on output, of potato in Plateau State, Nigeria. It was carried out in four Local Government Areas Jos south, Bokkos, Mangu and Barkin Ladi were purposively randomly selected from eight noted for potato production. The sample size was 240 households but only 182 ...

  19. Construction and use of a physical map of potato

    NARCIS (Netherlands)

    Borm, T.J.A.

    2008-01-01

    Feeding the growing world population is one of the biggest challenges for the 21st century.
    Potato, being the fourth crop in the human diet, after maize, wheat and rice, plays an
    important role in this respect. Like other crops, potato is exposed to a range of potentially

  20. Targeted mutagenesis using CRISPR/Cas in inbred potatoes

    Science.gov (United States)

    Targeted mutagenesis using sequence-specific nucleases (SSNs) has been well established in several important crop species, but is in need of improvement in potato (Solanum tuberosum L.). For over a century, potatoes have been bred as autotetraploids (2n = 4x = 48), relying on F1 selections and clona...

  1. Effect of selection methods on seed potato quality

    NARCIS (Netherlands)

    Gunadi, N.; Pronk, A.A.; Karjadi, A.K.; Prabaningrum, L.; Moekasan, T.K.

    2017-01-01

    Potato is one of the most important crops in the world. Although it ranks fourth after rice, wheat and maize, the major food crops in the world, either in production or in the economic value, in terms of energy and protein production per hectare and per unit of time, the potato ranks first which is

  2. Performance stability of potato genotypes under rainfed and ...

    African Journals Online (AJOL)

    YDessalegn

    system. However, currently varieties such as. Zengena and Guasa can be recommended for both production systems. Key words: Potato; variety; yield; rainfed; irrigation; stability; Ethiopia. 1. Introduction. Potato (Solanum tuberosum L.) is one of the most important vegetable crops grown in the high and mid altitude areas of.

  3. Senescence sweetening of chip and fry processing potatoes

    Science.gov (United States)

    Potato storage makes the crop available over an extended time period, but increases financial risk to growers and end users. Senescence sweetening limits storage duration for chip and fry processing potatoes because it results in an unacceptable accumulation of reducing sugars that result in dark-co...

  4. University of Florida potato variety trials spotlight: 'Harley Blackwell'

    Science.gov (United States)

    ‘Harley Blackwell’ is a potato variety that is commonly grown for the potato chip market. It was selected from the progeny of a cross between B0155-24 and B9935-8, and tested under the pedigree B0564-8. It was released and named jointly by the Agricultural Research Service, United States Department ...

  5. Shooting responses of potato ( Solanum tuberosum L.) varieties in ...

    African Journals Online (AJOL)

    A study was conducted in 2012 and 2013 at IRAD Bambui (Cameroon) to evaluate the rate of shooting in four improved IRAD potato varieties: Cipira, Tubira, Mafo and Bambui wonder. Liquid and solid media stocks prepared according to the International Potato Center (CIP)'s protocol were used. A randomized complete ...

  6. Radiosensitivity of germs, grafts and vegetating potato plants

    International Nuclear Information System (INIS)

    Kutovenko, L.N.; Serebrenikov, V.S.

    1977-01-01

    Sensitivity of germs, grafts and vegetating potato plants to γ-radiation with doses of 1, 2, 3 and 5 kR has been investigated. While radiation dose increases the percentage of the survived buds and grafts decreases. Radiosensitivity of potato plants is affected not only by a radiation dose but also by the physiological state of the plant

  7. A strategy for integrated low-input potato production

    NARCIS (Netherlands)

    Vereijken, P.H.; Loon, van C.D.

    1991-01-01

    Current systems of potato growing use large amounts of pesticides and fertilizers; these inputs are costly and cause environmental problems. In this paper a strategy for integrated low-input potato production is developed with the aim of reducing costs, improving product quality and reducing

  8. New varieties of potato can feed the poor | IDRC - International ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    2013-09-23

    Sep 23, 2013 ... Crops such as potato have the potential to feed and contribute to the food security of vulnerable populations who depend on this ancestral tuber for their ... of their potato crop income on chemicals to control late blight and other diseases — a high cost that also has environmental and health consequences.

  9. Influence of ventilation and media on potato ( Solanum tuberosum L ...

    African Journals Online (AJOL)

    Considering the most suitable relationship between ventilation and media in this study, two mixtures of perlite and coir (3:1, light and 1:3, heavy v/v) were applied to determine the growth characteristics var. Sante of potato plantlets. As potato has high potential in minituber production, different levels of supplemental aeration ...

  10. Identification and distribution of viruses infecting sweet potato ...

    African Journals Online (AJOL)

    Reverse-transcription polymerase chain reaction of total plant RNA isolations confirmed the presence of SPFMV, SPVG, SPCSV and SPMMV as the most prevalent viruses infecting sweet potato in KZN. Keywords: reverse-transcription polymerase chain reaction, sweet potato, viruses. South African Journal of Plant and Soil ...

  11. Potato bacterial wilt management in the Central Highlands of Ethiopia

    African Journals Online (AJOL)

    Farmers are not aware of the causes of potato diseases and they believe that all types of diseases are caused by rain and mist. ... sources and management options of the disease and assist farmers to develop healthy potato farms; Farmers field school(FFS) extension approach supported by sessions harmonized with crop ...

  12. Reactions of some potato genotypes to late blight in Cameroon ...

    African Journals Online (AJOL)

    ... evaluate reactions of different potato genotypes to late blight. There were significant differences among genotypes for tuber yield, late blight readings and earliness. The application of fungicides significantly increased potato yield but had a non significant effect on the damage due to foliage blight (Phytophthora infestans).

  13. Genetic diversity and population structure of begomoviruses infecting sweet potato

    Science.gov (United States)

    Begomoviruses infecting sweet potatoes (Ipomoea batatas) exhibit high genetic diversity, and approximately eight species including Sweet potato leaf curl virus (SPLCV) have been described from different regions around the world. In this study, the complete genomic sequences of 17 geographically dist...

  14. Analysis of weed flora in conventional and organic potato production

    Directory of Open Access Journals (Sweden)

    Nikolić, Lj.

    2013-12-01

    Full Text Available Composition of weed flora is highly dynamic and depends upon great number of factors, of which cultural practices that are applied by humans in certain crops are the most important. One of the most frequently grown plants in the world and in our country is potato (Solanum tuberosum L., Solanaceae, due to its high biological and nutritive value. Therefore, in the paper was presented taxonomic analysis of weed flora in potato grown conventionally and according to the principles of organic agricultural production, with the intention to point out to eventual differences between present weeds. Of the total number of identified species, from phylum Equisetophyta and class Equisetopsida, in organic potato crop, was determined only one, Equisetum arvense. Of remaining 38 weeds from phylum Magnoliophyta., classified into two classes, Magnoliopsida and Liliopsida. On both of potato growing systems, 39 weed species were found, classified into 16 families and 32 genus. Of the total number, 31 species was identified in conventional potato crop, and only 23 species in potato crop grown according to organic principles, which is for about quarter less. Biological spectrum of weed flora in both potato growing systems is pronouncedly of terrophytic – geophytic type. In the spectrum of area types were recorded differences, i.e. in the conventional potato crop represented are only widely distributed species, while in the organic crop, beside species of wide distribution are also present elements of Pontic group.

  15. Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments.

    Science.gov (United States)

    Abadias, M; Usall, J; Anguera, M; Solsona, C; Viñas, I

    2008-03-31

    A survey of fresh and minimally-processed fruit and vegetables, and sprouts was conducted in several retail establishments in the Lleida area (Catalonia, Spain) during 2005-2006 to determine whether microbial contamination, and in particular potentially pathogenic bacteria, was present under these commodities. A total of 300 samples--including 21 ready-to-eat fruits, 28 whole fresh vegetables, 15 sprout samples and 237 ready-to-eat salads containing from one to six vegetables--were purchased from 4 supermarkets. They were tested for mesophilic and psychrotrophic aerobic counts, yeasts and moulds, lactic acid bacteria, Enterobacteriaceae, presumptive E. coli and Listeria monocytogenes counts as well as for the presence of Salmonella, E. coli O157:H7, Yersinia enterocolitica and thermotolerant Campylobacter. Results for the fresh-cut vegetables that we analyzed showed that, in general, the highest microorganism counts were associated with grated carrot, arugula and spinach (7.8, 7.5 and 7.4 log cfu g(-1) of aerobic mesophilic microorganisms; 6.1, 5.8 and 5.2 log cfu g(-1) of yeast and moulds; 5.9, 4.0 and 5.1 log cfu g(-1) lactic acid bacteria and 6.2, 5.3 and 6.0 log cfu g(-1) of Enterobacteriaceae). The lowest counts were generally associated with fresh-cut endive and lettuce (6.2 and 6.3 log cfu g(-1) of aerobic mesophilic microorganisms; 4.4 and 4.6 log cfu g(-1) of yeast and moulds; 2.7 and 3.8 log cfu g(-1) lactic acid bacteria and 4.8 and 4.4 log cfu g(-1) of Enterobacteriaceae). Counts of psychrotrophic microorganisms were as high as those of mesophilic microorganisms. Microbiological counts for fresh-cut fruit were very low. Sprouts were highly contaminated with mesophilic (7.9 log cfu g(-1)), psychrotrophic microorganisms (7.3 log cfu g(-1)) and Enterobacteriaceae (7.2 log cfu g(-1)) and showed a high incidence of E. coli (40% of samples). Of the samples analyzed, four (1.3%) were Salmonella positive and two (0.7%) harboured L. monocytogenes. None of the

  16. Optimization of germination time and heat treatments for enhanced availability of minerals from leguminous sprouts.

    Science.gov (United States)

    Bains, Kiran; Uppal, Veny; Kaur, Harpreet

    2014-05-01

    Germinated legumes are highly nutritious food especially for their enhanced iron bioavailability primarily because of reduction of phytates and increase in ascorbic acid with an advancement of germination period. Length of germination time followed by different heat treatments affect the nutritive value of leguminous sprouts. To optimize germination time and heat treatments for enhanced availability of iron from leguminous sprouts, three legumes namely, mungbean, chickpea and cowpea were germinated for three time periods followed by cooking of sprouts by two cooking methods ie. pressure cooking and microwaving. Optimized germination time for mungbean was 12, 16 and 20 h; 36, 48 and 60 h for chickpea and 16, 20 and 24 h for cowpea. Germination process increased ascorbic acid significantly in all the three legumes, the values being 8.24 to 8.87 mg/100 g in mungbean, 9.34 to 9.85 mg/100 g in chickpea and 9.12 to 9.68 mg/100 g in cowpea. Soaking and germination significantly reduced the phytin phosphorus in all the three legumes, the percent reduction being 5.3 to 16.1% during soaking and 25.7 to 46.4% during germination. The reduction in phytin phosphorus after pressure cooking was 9.6% in mungbean, 18.4% in chickpea and 6.1% in cowpea. The corresponding values during microwaving were 8.4, 19.7 and 4.5%. Mineral bioavailability as predicted by phytate:iron enhanced significantly with an increase in germination time. Further reduction i.e. 0.9 to 16.3% was observed in three legumes after the two heat treatments. The study concluded that the longer germination periods ie. 20 h for mungbean, 60 h for chickpea and 24 h for cowpea followed by pressure cooking for optimized time were suitable in terms of better iron availability.

  17. Transmission of potato purple top phytoplasma to potato tubers and daughter plants

    Science.gov (United States)

    In 2006, 2007, and 2008, Alturas, Russet Burbank, FL1867, FL1879, Russet Norkotah, Ranger Russet, Shepody, and Umatilla Russet potatoes were planted at a research farm near Moxee, WA. Plants were allowed to become infested with native populations of the beet leafhopper which are known vectors of t...

  18. Potato production and innovative technologies. Proceedings Potato Russia International Conference, Moscou Agust 21-22, 2007

    NARCIS (Netherlands)

    Haverkort, A.J.; Anisimov, B.V.

    2007-01-01

    This comprehensive book is the result of the Potato Russia international conference that took place in August 2007 in Moscow. It begins with a series of papers that give an excellent overview of consumer behaviour and marketing with examples from various countries in the world. The quality of

  19. Physical properties of acetylated and enzyme-modified potato and sweet potato flours.

    Science.gov (United States)

    Yadav, A R; Guha, M; Reddy, S Y; Tharanathan, R N; Ramteke, R S

    2007-06-01

    Textural profile, pasting behavior, gelatinization characteristics, sedimentation volume, and gel consistency of acetylated (Ac) and enzyme (glucoamylase)-modified (EM) potato and sweet potato flours have been investigated to determine their suitability in products such as baked goods, soup, and pudding. Dough hardness of Ac and EM samples was significantly higher than their native samples (P potato did not change, while it decreased in modified sweet potato. With increase in moisture, textural properties of modified samples, in general, showed reduced values. Rapid Visco Analyser showed least pasting viscosities of Ac flours due to restricted swelling of starch granules while EM flours exhibited high viscosities. Acetylated samples showed reduced gelatinization temperature (GT), and enthalpy (DeltaH) compared to native samples, whereas enzyme-treated samples showed no significant changes in GT, indicating their comparable crystallinity values with those of native samples. Modified flour samples had lower sediment volumes and gel consistency, and the gel consistency of EM flour correlated with its cold paste viscosity.

  20. Texture of cooked potatoes (Solanum tuberosum). 2. Changes in pectin composition during storage of potatoes

    NARCIS (Netherlands)

    Dijk, van C.; Beekhuizen, J.G.; Gibcens, T.; Boeriu, C.; Fischer, M.; Stolle-Smits, T.

    2002-01-01

    During the storage season changes in the chemical composition of the pectin moiety of the cell walls of raw potatoes were studied. This compositional analysis was performed for the cultivars Nicola and Irene, which represent two extremes with regard to sensory-perceived texture. Both cultivars were