WorldWideScience

Sample records for prepared annual sers

  1. Facile Nanostructured Substrate Preparation Using Gold Nanocuboids for SERS

    Directory of Open Access Journals (Sweden)

    Pablo Genaro Martínez-Torres

    2015-04-01

    Full Text Available In the present work, we report a practical fabrication method of gold-aluminium substrates for surface en‐ hanced Raman spectroscopy. A commercial aluminium- foil was used to fabricate SERS substrates by depositing gold nanocuboids and faceted particles onto their surfaces using the drop-drying method. The gold nanoparticles were characterized by ultraviolet-visible spectroscopy, while the size and morphology were determined by field emission scanning electron microscopy. The perform‐ ance of the substrates was investigated using Rhoda‐ mine 6G in a water solution where a volume of 3 μl was placed on the surfaces of the SERS substrates, and the Raman spectra were immediately acquired using diode laser excitation at 785 nm. The estimated analytical enhancement factor of the gold-aluminium substrates was 1.8 x 106, using a solution of Rhodamine 6G with a concentration of 1 x 109 (0.4796 ppb. We show that our SERS substrates can be easily fabricated, and that they are reproducible and have suitable surface uniformity, thus allowing one to analyse Rhodamine 6G not only as a dry sample, but also in a solution.

  2. Preparation of sensitive and recyclable porous Ag/TiO2 composite films for SERS detection

    Science.gov (United States)

    Zhang, Zhengyi; Yu, Jiajie; Yang, Jingying; Lv, Xiang; Wang, Tianhe

    2015-12-01

    Porous Ag/TiO2 composite films were prepared by spin coating of titania on normal glass slides and subsequent photochemical deposition of silver nanoparticles (AgNPs). The films were characterized by XRD and FESEM to reveal micro structural and morphological differences between films obtained under varied conditions. The SERS properties of these films were investigated using aqueous crystal violet (CV) as probe molecules. The results indicate that the content of polyethylene glycol (PEG) and photo-reduction time had significant influences on both the microstructure and SERS performance of Ag/TiO2 films. The highest SERS sensitivity that allowed as low as 10-10 M aqueous CV to be detected, was achieved with the PEG/(C4H9O)4Ti molar ratio being 0.08% and with 30 min of UV irradiation. With this film a linear relationship was established through experiment between SERS intensity and CV concentration from 10-10 to 10-5 M, which could be used as a calibration curve for CV concentration measurement. In addition, the film could be reused as a SERS substrate for up to four times without significantly losing SERS sensitivity if a simple regeneration was followed. It is visualized that the Ag/TiO2 film on glass has potentials for being developed into a practical SERS substrate with high sensitivity and good reusability.

  3. Surface Enhanced Raman Scattering (SERS Studies of Gold and Silver Nanoparticles Prepared by Laser Ablation

    Directory of Open Access Journals (Sweden)

    Samuel P. Hernandez-Rivera

    2013-03-01

    Full Text Available Gold and silver nanoparticles (NPs were prepared in water, acetonitrile and isopropanol by laser ablation methodologies. The average characteristic (longer size of the NPs obtained ranged from 3 to 70 nm. 4-Aminobenzebethiol (4-ABT was chosen as the surface enhanced Raman scattering (SERS probe molecule to determine the optimum irradiation time and the pH of aqueous synthesis of the laser ablation-based synthesis of metallic NPs. The synthesized NPs were used to evaluate their capacity as substrates for developing more analytical applications based on SERS measurements. A highly energetic material, TNT, was used as the target compound in the SERS experiments. The Raman spectra were measured with a Raman microspectrometer. The results demonstrate that gold and silver NP substrates fabricated by the methods developed show promising results for SERS-based studies and could lead to the development of micro sensors.

  4. Optical Characterization of SERS Substrates Based on Porous Au Films Prepared by Pulsed Laser Deposition

    Directory of Open Access Journals (Sweden)

    V. V. Strelchuk

    2015-01-01

    Full Text Available The SERS (surface enhanced Raman spectroscopy substrates based on nanocomposite porous films with gold nanoparticles (Au NPs arrays were formed using the method of the pulsed laser deposition from the back low-energy flux of erosion torch particles on the glass substrate fixed at the target plain. The dependencies of porosity, and morphology of the surface of the film regions located near and far from the torch axis on the laser ablation regime, laser pulses energy density, their number, and argon pressure in the vacuum chamber, were ascertained. The Au NPs arrays with the controllable extinction spectra caused by the local surface plasmon resonance were prepared. The possibility of the formation of SERS substrates for the detection of the Rhodamine 6G molecules with the concentration 10−10 Mol/L with the enhancement factor 4·107 was shown.

  5. Facile preparation of SERS and catalytically active Au nanostructures using furfuryl derivatives

    Science.gov (United States)

    Kim, Ki-Jung; Kim, Hyun-Chul; Park, Minsun; Huh, Seong

    2017-08-01

    Six different types of Au nanostructures with rough surfaces were readily prepared through the redox reactions between Au precursor, AuCl4-, and furfuryl derivatives without extra metal surface capping ligands, in deionized water at room temperature. Furfuryl alcohol (FA) or furfurylamine (FFA) was used as a sole reducing agent for the reduction of Au precursor. Both FA and FFA effectively polymerized during the redox reactions to form polyfuran polymers. These polymers are thought to act as surface capping ligands during the formation of Au nanostructures. Experiments were conducted with three different concentrations of each furfuryl derivative. Interestingly, Au particles prepared from the reaction with varying concentration of FA or FFA showed large differences in size, and revealed that the higher the ratios of [FA]/[AuCl4-] or [FFA]/[AuCl4-], the smaller the size of Au particles. The size of Au particles was in the range of 1 μm to under 30 nm. Among these samples, two nanostructured Au particles, AuFA-4 and AuFFA-1, deposited on a Si wafer by a simple drop-casting method, were revealed as highly active surface-enhanced Raman scattering (SERS) substrates for the detection of methylene blue (MB) and crystal violet (CV). High SERS enhancement factors (EFs) of 106 ∼ 108 for MB and CV were observed. Small size Au nanoparticles (AuFFA-2 and AuFFA-4) were also found to be very active for the catalytic hydrogenation of 4-nitrophenol to 4-aminophenol in the presence of NaBH4 at room temperature. AuFFA-2 could be recycled eight times, without losing its activity.

  6. Optimization of the preparation of glass-coated, dye-tagged metal nanoparticles as SERS substrates.

    Science.gov (United States)

    Brown, Leif O; Doorn, Stephen K

    2008-03-04

    Dye-tagged metal nanoparticles are of significant interest in SERS-based sensitive detection applications. Coating these particles in glass results in an inert spectral tag that can be used in applications such as flow cytometry with significant multiplexing potential. Maximizing the SERS signal obtainable from these particles requires care in partitioning available nanoparticle surface area (binding sites) between the SERS dyes and the functionalized silanes necessary for anchoring the glass coating. In this article, we use the metal-mediated fluorescence quenching of SERS dyes to measure surface areas occupied by both dyes and silanes and thus examine SERS intensities as a function of both dye and silane loading. Notably, we find that increased surface occupation by silane increases the aggregative power of added dye but that decreasing the silane coverage allows a greater surface concentration of dye. Both effects increase the SERS intensity, but obtaining the optimum SERS intensity will require balancing aggregation against surface dye concentration.

  7. Preparing for your annual staff appraisal: part 2.

    Science.gov (United States)

    Price, B

    Annual appraisals are part of performance management and are designed to motivate, develop and support employees in performing their roles to the highest possible standard. They provide an opportunity for constructive discussion of performance, identification of areas for development and agreement of approaches by which employees needs could be met. Part 1 of this article was concerned with preparation of the employee and manager for annual staff appraisal. This article provides advice for managers who perform annual appraisal interviews. Guidance is offered on how to ensure the strategic objectives of the team and healthcare organisation are met, a balanced understanding of nurse performance is achieved, and future objectives are identified and agreed

  8. Rough SERS substrate based on gold coated porous silicon layer prepared on the silicon backside surface

    Science.gov (United States)

    Dridi, H.; Haji, L.; Moadhen, A.

    2017-04-01

    We report in this paper a novel method to elaborate rough Surface Enhanced Raman Scattering (SERS) substrate. A single layer of porous silicon was formed on the silicon backside surface. Morphological characteristics of the porous silicon layer before and after gold deposition were influenced by the rough character (gold size). The reflectance measurements showed a dependence of the gold nano-grains size on the surface nature, through the Localized Surface Plasmon (LSP) band properties. SERS signal of Rhodamine 6G used as a model analyte, adsorbed on the rough porous silicon layer revealed a marked enhancement of its vibrational modes intensities.

  9. 12 CFR 620.4 - Preparing and providing the annual report.

    Science.gov (United States)

    2010-01-01

    ... SHAREHOLDERS Annual Report to Shareholders § 620.4 Preparing and providing the annual report. (a) Each... Administration; (3) Provide prior written notification to its shareholders that the institution will publish its... shareholders an annual report substantively identical to the copy of the report sent to the Farm...

  10. Deber Ser

    OpenAIRE

    González, A M

    2010-01-01

    1. Ontología del deber ser. 2. Continuidad y diferencia entre consideración ontológica y moral del deber ser. 3. Deber ser moral: a) Relación entre bien y deber; b) Relación entre deber y ley. 4. Dimensión subjetiva y objetiva o institucional del deber. 5. El mal moral: apartarse de la norma/apartarse del fin

  11. Preparation of Fe3O4@Ag SERS substrate and its application in environmental Cr(VI) analysis.

    Science.gov (United States)

    Du, Jingjing; Jing, Chuanyong

    2011-06-01

    A novel sensitive and recyclable SERS substrate which can actively concentrate chromate (Cr(VI)) in water and substantially enhance Raman signal was synthesized as uniform Fe(3)O(4)@Ag nanoparticles. The surface morphology, structure, and magnetic properties were characterized using transmission electron microscopy, atomic force microscopy, X-ray powder diffraction, X-ray photoelectron spectroscopy, and vibrating sample magnetometry analysis. The closely spaced Fe(3)O(4)@Ag substrate with a core-shell structure exhibited a 25 nm surface roughness. The high saturation magnetization at 48.35 emu g(-1) enabled the complete and rapid separation of the substrate from the solution. The sensitivity and reproducibility of the substrate were confirmed using a common SERS probe molecule, rhodamine 6G. SERS spectra of Cr(VI) in simulated and real contaminated water showed that the symmetric stretching vibrations of Cr-O occurred at 796 cm(-1). This SERS peak area exhibited a linear dependence (R(2)=0.9992) on the Cr(VI) concentration between 5 and 100 μg L(-1). Coexisting anions such as sulfate, nitrate, chloride, carbonate, and humic acid could decrease the sensitivity of the SERS analysis. However, the adverse effect of the competing ions may be eliminated by proper dilution of the raw sample. This study provides a reliable method for qualitative and quantitative analysis of Cr(VI).

  12. Sixth annual coal preparation, utilization, and environmental control contractors conference

    Energy Technology Data Exchange (ETDEWEB)

    1990-01-01

    A conference was held on coal preparation, utilization and environmental control. Topics included: combustion of fuel slurries; combustor performance; desulfurization chemically and by biodegradation; coal cleaning; pollution control of sulfur oxides and nitrogen oxides; particulate control; and flue gas desulfurization. Individual projects are processed separately for the databases. (CBS).

  13. 78 FR 47829 - Notice of Open Meetings To Prepare 2013 Annual Report to Congress

    Science.gov (United States)

    2013-08-06

    ... policy and U.S. access to China's markets. China's impact on U.S. security interests, including a... SECURITY REVIEW COMMISSION Notice of Open Meetings To Prepare 2013 Annual Report to Congress Advisory Committee: U.S.-China Economic and Security Review Commission. ACTION: Notice of open meetings to be held...

  14. 二氧化硅-金核壳材料的制备与SERS研究%Preparation of SiO2@Au Nanoshell for SERS Study

    Institute of Scientific and Technical Information of China (English)

    陈雷; 薛向欣; 刘洋; 高铭; 王雅新; 杨景海

    2015-01-01

    表面增强拉曼散射( SERS)活性基底的制备与优化对靶分子的研究非常关键.本文设计了一种二氧化硅—金核壳材料的SERS活性基底,通过调节二氧化硅—金种子和金生长溶液的比例调节二氧化硅球表面金纳米材料的厚度,得到一系列表面等离子体吸收带可调控的SERS活性基底.这类材料具有较宽的吸收带,适用于多激发线激发,并产生强烈的表面等离子体共振吸收峰.我们采用633 nm激发波长,选择苯硫酚作为探针分子研究基底的SERS活性,其SERS增强因子可以达到107. SERS的增强贡献主要来源于电磁场增强,而聚集在二氧化硅纳米粒子表面的金会提供巨大的电磁场,提高了基底的检测灵敏度和光谱重现性.这类基底在基于SERS应用方面具有潜在的应用价值.%In this paper,we prepared a SiO2@ Au nanoshell substrate for surface-enhanced Raman scattering study. In SERS-based study,SERS substrate was an important part for the determination of the target. By adjusting the proportional of SiO2@Au and K-gold solutions to control the thickness of the gold shell which was covered on the SiO2 particles. UV-vis-NIR absorption spectrometer and SEM were employed to monitor the reaction process of the formation of Au on the surface of silica bead,and the optical resonance of the substrate could turn from visible to NIR area. It possessed the broad surface plasmon resonance,which extend the SERS applications with different laser excitations. To investigate the potential of this substrate for SERS applications, SERS spectra of thiophenol( TP )were normalized by using an internal standard of silicon( 520 cm-1 ). The calculated enhancement factor( EF)of TP adsorbed onto a SiO2@Au core-shell substrate was ~107 following 633 laser excitations. It could effectively control surface morphology of SiO2@Au core-shell,which was one of the important factors in the fabrication of an excellent SERS

  15. Ser maestro

    Directory of Open Access Journals (Sweden)

    Néstor Alonso Sánchez Cardozo

    2015-10-01

    Full Text Available El autor nos presenta una visión formativa de la manera de cómo abordar el trabajo monográfico para obtener el título de licenciado. Su objetivo es el de establecer unas reglas de escritura para la realización de este tipo de ensayo, como un hacer pedagógico de varias manos, donde puedan participar docentes y estudiantes. A través de este ejercicio formativo se viene a estructurar un tipo de escritura, para hacer de él, la razón de ser del futuro egresado en educación: escribir acerca de la cotidianidad escolar.AbstractThe author shows us a formative view of the way about how to board the monographic work in order to get a graduate degree. His objective is to establish some writing rules for this type of essay, as a daily task accomplished by several persons, where educators and students can participate. Through this exercise a type of writing is structured so that it will become the raison d´etre of the graduate-to-be in education : writing about the school daily life.

  16. Ser reina

    Directory of Open Access Journals (Sweden)

    José Manuel NIETO SORIA

    2006-07-01

    Full Text Available L’historiographie du règne des Rois Catholiques, héritière directe de celle des autres Trastamare, se caractérise par son étroite relation avec des enjeux politiques concrets. L’activité historiographie s’est ainsi inscrite elle-même dans le cadre des conflits politiques en cours. C’est pourquoi la royauté d’Isabelle Ire de Castille impliqua une bonne part de la production historiographique de cettte époque : soit qu’on dénonçât un déficit de légitimité dû à sa condition féminine, soit qu’on démentît, au contraire, ce déficit en attribuant à la reine des qualités « masculines ». Bien entendu, ces débats furent fonction de l’engagement politique de chacun des historiens.La cronística y la historiografía del reinado de los Reyes Católicos, como directas herederas de la labor historiográfica de la época de los monarcas Trastámara, se caracterizó por su estrecha vinculación con intereses políticos concretos, inscribiéndose la propia actividad historiográfica en el marco de los conflictos políticos en curso. Por ello, la dimensión regia de Isabel I de Castilla supuso una dimensión significativa del quehacer historiográfico de la época, bien para plantear un déficit de legitimidad por razón de su propia condición femenina, bien para negar tal déficit con la atribución de “cualidades masculinas” en su persona. De este modo, la toma en consideración del hecho de “ser reina” representó una dimensión significativa del quehacer historiográfico, de acuerdo siempre con los compromisos políticos de los distintos historiadores de la época.

  17. Security effectiveness review (SER)

    Energy Technology Data Exchange (ETDEWEB)

    Kouprianova, I. [Inst. of Physics and Power Engineering, Obninsk (Russian Federation); Ek, D.; Showalter, R. [Sandia National Labs., Albuquerque, NM (United States); Bergman, M. [Lawrence Livermore National Lab., CA (United States)

    1998-08-01

    As part of the on-going DOE/Russian MPC and A activities at the Institute of Physics and Power Engineering (IPPE) and in order to provide a basis for planning MPC and A enhancements, an expedient method to review the effectiveness of the MPC and A system has been adopted. These reviews involve the identification of appropriate and cost-effective enhancements of facilities at IPPE. This effort requires a process that is thorough but far less intensive than a traditional vulnerability assessment. The SER results in a quick assessment of current and needed enhancements. The process requires preparation and coordination between US and Russian analysts before, during, and after information gathering at the facilities in order that the analysis is accurate, effective, and mutually agreeable. The goal of this paper is to discuss the SER process, including the objectives, time scale, and lessons learned at IPPE.

  18. The SERS Detection of SudanⅠby Using AAO as Template to Prepare the Substrate%利用AAO模板制备SERS基底检测SudanⅠ

    Institute of Scientific and Technical Information of China (English)

    潘晓会; 张芹; 郭伟; 陈发河

    2015-01-01

    The large‐scale controllable ,ordered two‐dimensional arrays of gold nanostructure with hot‐spot were prepared to‐gether with chemical molecules were modified on the surface to concentrate Sudan Ⅰwithin the zone of the SERS effect ,which lead to analytical detection of SudanⅠin high resolution .The vapor of gold was deposed on anodic aluminum oxide(AAO)tem‐plate by ~200 nm thickness to replicate its nanochannels ,and the negative structure i .e .large‐scale ordered gold nano‐hemi‐sphere array ,was obtained after the removal of the template of AAO by NaOH solution .Au nano‐hemisphere array was modi‐fied by 1‐Dodecanethiol which can be self‐assembled monolayer on the surface and concentrate Sudan Ⅰ within the zone of the SERS detection ,which can facilitate the measurement of Sudan Ⅰ .Due to the order and regularity of Au nano‐hemisphere array , the signal of SudanⅠin the range of laser illumination is stable and uniform ,and the quantitative analysis of SudanⅠwas real‐ized .The SERS intensity of SudanⅠis logistic proportional to the concentration in the range of 10-7 to 5 × 10-4 mol・L -1 .The corresponding correlation coefficient of the liner equation is 0.99 ,the recoveries of SudanⅠare between 77% ~117% .The limit of detection for SudanⅠis 4 × 10-8 mol・L -1 ,comparable to that of HPLC of Chinese national standard method .%通过模板法制备大面积、可控的、可重复的、热点集中的金纳米结构阵列,并在纳米结构阵列上通过化学修饰分子,吸附更多苏丹红Ⅰ分子至金纳米的SERS增强区域,实现其高灵敏的表面增强拉曼分析检测。以多孔阳极氧化铝为模板,通过真空蒸镀金,约200 nm厚度,复制氧化铝的孔洞结构,用碱液将氧化铝模板腐蚀去除,可得到氧化铝模板的互补结构,即大面积的、均匀的金半球纳米结构阵列。在金纳米结构阵列上修饰十二硫醇,硫醇巯基端与纳米金相

  19. Preparation of Gold Nanoparticle Aggregates and Characterization for Their SERS Effect%金纳米粒子聚集体系的制备及其SERS效应表征

    Institute of Scientific and Technical Information of China (English)

    李玲; 曹晓卫; 李欣然; 张志学; 冯小平

    2013-01-01

    This article used Na2S as a chemical reduction and aggregation agent to prepare unique gold nanoparticle aggregates under the room-temperature condition. By ultraviolet-visible spectroscopy, the preparation procedure and technological conditions were optimized for the purpose of preparation of steady aggregates. The transmission electron microscopic images display that the as-prepared gold nanoparticles are spherical and the gold nanoparticles are gathered so effectively that these particles can finally form well-defined aggregates. Furthermore, the as-prepared aggregates were used as the substrates of surface-enhanced Raman scattering (SERS) measurements to characterize several amino acid molecules such as glycine and histidine. Previous investigations show that the as-prepared gold nanoparticle aggregates possess so good SERS activity and this make it useful for SERS analysis of a wide variety of biomolecules.%以Na2S作为金纳米粒子的还原制备与聚集剂制备了金纳米粒子聚集体系.在制备过程中通过紫外可见光谱对制备条件进行了优化.TEM表征显示聚集体系中的金纳米粒子均为球形,且聚集状态呈现出较好的均匀性.将这一聚集体系作为SERS基底应用于若干氨基酸分子的SERS光谱表征与分析.初步的研究表明Na2S-金纳米粒子聚集体系可有效地应用于生物分子的SERS光谱表征与分析.

  20. Preparation of Graphene Oxide Decorated Fe3O4@SiO2 Nanocomposites with Superior Adsorption Capacity and SERS Detection for Organic Dyes

    Directory of Open Access Journals (Sweden)

    Song Yang

    2015-01-01

    Full Text Available The fast detection and removal of organic dyes from contaminated water has become an urgent environmental issue due to their high toxicity, chemical stability, and low biodegradability. In this paper, we have developed graphene oxide decorated Fe3O4@SiO2 (Fe3O4@SiO2-GO as a novel adsorbent aiming at the rapid adsorption and trace analysis of organic dyes followed by surface enhanced Raman scattering (SERS. The structure and morphology of the nanocomposites were characterized by transmission electron microscopy (TEM, Fourier infrared spectrometry (FT-IR, X-ray diffraction (XRD, and vibrating sample magnetometer (VSM. The obtained nanocomposites were used to adsorb methylene blue (MB in aqueous solution based on π-π stacking interaction and electrostatic attraction between MB and GO, and the adsorption behaviors of MB were investigated. Moreover, the obtained nanocomposites with adsorbed dyes were separated from the solution and loaded with silver nanoparticles for SERS detection. These nanocomposites showed superior SERS sensitivity and the lowest detectable concentration was 1.0 × 10−7 M.

  1. Ser humano: Ser-en-la-existencia

    Directory of Open Access Journals (Sweden)

    Víctor Ricardo Moreno Holguín

    2014-01-01

    Full Text Available Es necesario superar el discurso sobre el SER y la Existencia para adentrarse en un camino práctico, que redescubre el dinamismo de la existencia de la persona humana como presencia encarnada del SER. Guiados por las palabras del místico Maestro Eckhart de Hochheim se redescubre al SER como Principio y Fundamento de la esencia de la perso- na, (SER al que buena parte de la humanidad ha llamado “Dios”, que por medio de diversas ma- nifestaciones percibidas a nivel psicológico, parece llamarlo al reencuentro. Por esto conviene ampliar la mirada para releer manifestaciones tales como la ansiedad y los estados emocionales incomprendidos por quienes los padecen y que la medicina tradicio- nal los trata como situaciones estáticas, como signos de un proceso dinámico en el alma. Así es nece- saria una psicología del Camino Interior durante el proceso que la persona realiza hasta acceder al Ser esencial. Este Camino Interior incluye la liberación de la dictadura de las emociones y de otras dolencias del alma. Finaliza este artículo con una anotación a una práctica que puede orientar hacia dónde dirigir la mirada cuando se opta por el Camino Interior; la práctica del sentarse, como no-acción y recogimien- to interior, el vivir con plena atención y mantener la respiración correcta, como se practica en muchos monasterios, que además de alejar del activismo y el frenesí de la cotidianidad, abre las puertas para el descubrimiento interior del ser humano como SER hecho Existencia

  2. Construction of annual cycle of preparation of sportsmen, specialized in cheerleading. [Postroenie godovogo cikla podgotovki sportsmenov, specializiruiushchikhsia v cherlidinge

    Directory of Open Access Journals (Sweden)

    Loutsenko L.S.

    2011-11-01

    Full Text Available The plan-chart of distributing of educational material is rotined in the annual loop of preparation of sportsmen. In an experiment took part 42 sportsmen in age 18-19 years. It is marked that duration of setup time is occupied by to 6 months of preparation. The following correlation of elements is recommended in the structure of competition period: 10% is a body-conditioning, 20% is the special physical preparation, 70% is technical preparation. It is marked that it is necessary to concentrate basic attention on working off competition composition, elements and synchronousness at implementation of compositions. It becomes firmly established that the transitional period of sportsmen is directed on renewal of physical and psychological potential. In this period it is recommended to bring in adjustments in competition compositions or make new.

  3. Use of trainings in mountain terms in the olympic annual loop of preparation in at run on midranges

    Directory of Open Access Journals (Sweden)

    Samolenko T.V.

    2012-03-01

    Full Text Available The variant of the use of training of sportsmen is presented in mountain terms. The basic going is exposed near planning of training in the annual loop of preparation of sportsmen. The features of correlation of facilities of preparation are considered at combination of training in middleland and highland. 4 collections are recommended in mountains in the year of leadthrough of Games of Olympiad in the following orientation: pulling (middleland in, intensive and power (highland, antihunt on a background sufficient intensity (highland, volume and intensive (middleland. Correlation of loadings of different orientation is rotined taking into account the individual features of to training of sportsmen.

  4. Separation followed by direct SERS detection of explosives on a novel black silicon multifunctional nanostructured surface prepared in a microfluidic channel

    DEFF Research Database (Denmark)

    Talian, Ivan; Hübner, Jörg

    2013-01-01

    The article describes the multifunctionality of a novel black silicon (BS) nanostructured surface covered with a thin layer of noble metal prepared in the a microfluidic channel. It is focused on the separation properties of the BS substrate with direct detection of the separated analytes utilizing...

  5. Management and control after the special preparation of skiers-racing drivers on the stages of annual macrocycle

    Directory of Open Access Journals (Sweden)

    Sidorova T.V.

    2012-01-01

    Full Text Available In theory and the estimation of the special preparation of skiers-racing drivers is experimentally grounded on the stages of annual cycle of training. The norms of the special preparedness of sportsmen are certain depending on style of movement. The levels of the special preparedness of sportsmen are certain in the whole-year training. It is well-proven that an orientation on evaluation scales allows objectively to characterize the state of separate components of motive function of every sportsman. Also to range and group sportsmen on typological the features of preparedness. Technology of correction of orientation of the trainings loadings is recommended.

  6. Valuing goodwill: not-for-profits prepare for annual impairment testing.

    Science.gov (United States)

    Heuer, Christian; Travers, Mary Ann K

    2011-02-01

    Accounting standards for valuing goodwill and intangible assets are becoming more rigorous for not-for-profit organizations: Not-for-profit healthcare organizations need to test for goodwill impairment at least annually. Impairment testing is a two-stage process: initial analysis to determine whether impairment exists and subsequent calculation of the magnitude of impairment. Certain "triggering" events compel all organizations--whether for-profit or not-for-profit--to perform an impairment test for goodwill or intangible assets.

  7. Tenth annual coal preparation, utilization, and environmental control contractors conference: Proceedings. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-01

    Volume I contains papers presented at the following sessions: high efficiency preparation; advanced physical coal cleaning; superclean emission systems; air toxics and mercury measurement and control workshop; and mercury measurement and control workshop. Selected papers have been processed for inclusion in the Energy Science and Technology Database.

  8. Western Kentucky University's Teacher Preparation Evaluation Model Phase I, Cycle I. Annual Report.

    Science.gov (United States)

    Adams, Ronald D.

    A teacher preparation evaluation model was developed at Western Kentucky University. A total of 18 secondary and 20 elementary student teachers participated in the study. Instrumentation and records used for data collection consisted of five general types: a questionnaire, a personality scale, rating scales, direct classroom observational systems,…

  9. Ninth annual coal preparation, utilization, and environmental control contractors conference: Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    Papers are grouped under the following sessions: compliance technology; high-efficiency preparation; characterization; advanced technologies; alternative fuels; coal utilization; industrial/commercial combustor development; combustion; superclean emission systems; carbon dioxide recovery and reuse; air toxics and fine particulates; air toxics sampling and analysis workshop; and combined poster session. Selected papers have been processed separately for inclusion in the Energy Science and Technology Database.

  10. Eleventh annual coal preparation, utilization, and environmental control contractors conference: Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    The 75 papers contained in this volume are divided into the following sections: compliance technology; technology base activities; high efficiency preparation; air toxics (especially mercury); air toxics and CO{sub 2} control; superclean emissions; Combustion 2000; advanced research; commercial and industrial combustion systems; alternative fuels; environmental control; and coal utilization. Selected papers are indexed separately for inclusion in the Energy Science and Technology Database.

  11. Development of SERS active fibre sensors

    CERN Document Server

    Polwart, E

    2002-01-01

    Surface-enhanced Raman scattering (SERS) is sensitive and selective and when coupled with fibre-optics could potentially produce an effective chemical sensing system. This thesis concerns the development of a single-fibre-based sensor, with an integral SERS-active substrate. A number of different methods for the manufacture of SERS-active surfaces on glass substrates were investigated and compared. The immobilisation of metal nanoparticles on glass functionalised with (3-aminopropyl)trimethoxysilane emerged as a suitable approach for the production of sensors. Substrates prepared by this approach were characterised using UV-visible spectroscopy, electron microscopy and Raman mapping. It was found that exposure of substrates to laser radiation led to a decrease in the signal recorded from adsorbed analytes. This speed of the decrease was shown to depend on the analyte, and the exciting wavelength and power. SERS-active fibre sensors were produced by immobilisation of silver nanoparticles at the distal end of a...

  12. for SERS and Photocatalytic Applications

    Directory of Open Access Journals (Sweden)

    Xue Chen

    2011-01-01

    Full Text Available ZnS/Si nanocables were synthesized via a simple two-step thermal evaporation method. The shape and diameter of the ZnS/Si nanocables can be controlled by adjusting the morphologies of the ZnS nanostructures (nanowire or nanoribbon obtained in the first step and the deposition time of the Si shell in the second step, respectively. Furthermore, we obtained polycrystalline Si nanotubes with different shapes and diameters by etching away the inner ZnS core. The as-prepared Si nanotubes were employed as SERS-active substrates, which exhibited a high sensitivity for the detection of R6G. The Si nanotubes also showed effective photocatalytic activity on the decomposition of R6G under the irradiation of visible light.

  13. Aprendendo a ser psicoterapeuta

    Directory of Open Access Journals (Sweden)

    Elizabeth Amelio Faleiros

    Full Text Available Este estudo investiga, na perspectiva de Jacob Levy Moreno, a concepção que alunos de Psicologia têm sobre o que é ser psicoterapeuta, quais elementos são necessários para o desenvolvimento dessa tarefa e os fatores impeditivos para realizá-la. Propõe formas de soluções para o desempenho daquela função, favorecendo a reflexão sobre a importância dessa tarefa e a responsabilidade do profissional junto ao paciente. A metodologia utilizada é a qualitativa, pois esta permite abordar dimensões da subjetividade dos sujeitos. Os resultados revelam que os alunos possuem em sua concepção os alicerces básicos, cujos indicadores são apontados por Moreno e por outros autores, percebem os requisitos básicos que constituem a essência do papel de terapeuta, evidenciam críticas realistas sobre os fatores limitadores e sugerem ações pedagógicas para minimizá-los.

  14. Tuning SERS for living erythrocytes

    DEFF Research Database (Denmark)

    Brazhe, Nadezda; Parshina, E.Y.; Khabanova, V.V.;

    2013-01-01

    Surface-enhanced Raman spectroscopy (SERS) is a unique technique to study submembrane hemoglobin (Hbsm) in erythrocytes. We report the detailed design of SERS experiments on living erythrocytes to estimate dependence of the enhancemen t factor for main Raman bands of Hbsm on silver nanoparticle (Ag...

  15. Preparation

    Directory of Open Access Journals (Sweden)

    M.M. Dardir

    2014-03-01

    Full Text Available Some hexanamide-mono and di-linoleniate esters were prepared by the reaction of linolenic acid and hexanamide (derived from the reaction of hexanoic acid and diethanolamine. The chemical structure for the newly prepared hexanamide-mono and di-linoleniate esters were elucidated using elemental analysis, (FTIR, H 1NMR and chemical ionization mass spectra (CI/Ms spectroscopic techniques. The results of the spectroscopic analysis indicated that they were prepared through the right method and they have high purity. The new prepared esters have high biodegradability and lower toxicity (environmentally friendly so they were evaluated as a synthetic-based mud (ester-based mud for oil-well drilling fluids. The evaluation included study of the rheological properties, filtration and thermal properties of the ester based-muds formulated with the newly prepared esters compared to the reference commercial synthetic-based mud.

  16. Vinte anos de efeito SERS Twenty years of SERS

    Directory of Open Access Journals (Sweden)

    Dalva L. A. de Faria

    1999-07-01

    Full Text Available The Surface Enhanced Raman Scattering (SERS effect was observed for the first time in 1974, but it was only considered a new effect three years later, hence, nearly twenty years ago. Since its discovery, a significant amount of investigations have been performed aiming at to clarify the nature of the observed enhancement, to improve the surface stability and to establish applications which nowadays range from the study of biomolecules to catalysis. Some of the more relevant aspects of this effect which have been examined across the last two decades are summarized in this paper which presents the introductory aspects of SERS alongside with several of its applications.

  17. Stamping SERS for creatinine sensing

    Science.gov (United States)

    Li, Ming; Du, Yong; Zhao, Fusheng; Zeng, Jianbo; Santos, Greggy M.; Mohan, Chandra; Shih, Wei-Chuan

    2015-03-01

    Urine can be obtained easily, readily and non-invasively. The analysis of urine can provide metabolic information of the body and the condition of renal function. Creatinine is one of the major components of human urine associated with muscle metabolism. Since the content of creatinine excreted into urine is relatively constant, it is used as an internal standard to normalize water variations. Moreover, the detection of creatinine concentration in urine is important for the renal clearance test, which can monitor the filtration function of kidney and health status. In more details, kidney failure can be imminent when the creatinine concentration in urine is high. A simple device and protocol for creatinine sensing in urine samples can be valuable for point-of-care applications. We reported quantitative analysis of creatinine in urine samples by using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) based SERS substrate. S-SERS technique enables label-free and multiplexed molecular sensing under dry condition, while NPGD provides a robust, controllable, and high-sensitivity SERS substrate. The performance of S-SERS with NGPDs is evaluated by the detection and quantification of pure creatinine and creatinine in artificial urine within physiologically relevant concentration ranges.

  18. Ultrasensitive sliver nanorods array SERS sensor for mercury ions.

    Science.gov (United States)

    Song, Chunyuan; Yang, Boyue; Zhu, Yu; Yang, Yanjun; Wang, Lianhui

    2017-01-15

    With years of outrageous mercury emissions, there is an urgent need to develop convenient and sensitive methods for detecting mercury ions in response to increasingly serious mercury pollution in water. In the present work, a portable, ultrasensitive SERS sensor is proposed and utilized for detecting trace mercury ions in water. The SERS sensor is prepared on an excellent sliver nanorods array SERS substrate by immobilizing T-component oligonucleotide probes labeled with dye on the 3'-end and -SH on the 5'-end. The SERS sensor responses to the specific chemical bonding between thymine and mercury ions, which causes the previous flexible single strand of oligonucleotide probe changing into rigid and upright double chain structure. Such change in the structure drives the dyes far away from the excellent SERS substrate and results in a SERS signal attenuation of the dye. Therefore, by monitoring the decay of SERS signal of the dye, mercury ions in water can be detected qualitatively and quantitatively. The experimental results indicate that the proposed optimal SERS sensor owns a linear response with wide detecting range from 1pM to 1μM, and a detection limit of 0.16pM is obtained. In addition, the SERS sensor demonstrates good specificity for Hg(2+), which can accurately identify trace mercury ions from a mixture of ten kinds of other ions. The SERS sensor has been further executed to analyze the trace mercury ions in tap water and lake water respectively, and good recovery rates are obtained for sensing both kinds of water. With its high selectivity and good portability, the ultrasensitive SERS sensor is expected to be a promising candidate for discriminating mercury ions in the fields of environmental monitoring and food safety.

  19. ser en ortodoncia

    OpenAIRE

    2014-01-01

    La primera aplicación del láser en un diente fue realizada en 1965. Desde entonces ha presentado una constante evolución y desarrollo. La tecnología láser permite realizar procedimientos en tejidos duros y blandos, pudiendo ser utilizado con las siguientes finalidades: como prevención de la desmineralización, en la adhesión y remoción de brackets, en la reducción del dolor producto del movimiento dental, en la reparación ósea después de la expansión, en diversas cirugías y otras aplicaciones ...

  20. ser en ortodoncia

    OpenAIRE

    Ruíz-Esculpi, María; Universidad Central de Venezuela. Caracas.; Ricse-Chaupis, Estela; Universidad de San Martín de Porres. Lima.; Villanueva-Vega, Judith; Universidad Nacional Mayor de San Marcos. Lima.; Torres-Maita, Liz; Facultad de Estomatología, Universidad Peruana Cayetano Heredia. Lima.

    2014-01-01

    La primera aplicación del láser en un diente fue realizada en 1965. Desde entonces ha presentado una constante evolución y desarrollo. La tecnología láser permite realizar procedimientos en tejidos duros y blandos, pudiendo ser utilizado con las siguientes finalidades: como prevención de la desmineralización, en la adhesión y remoción de brackets, en la reducción del dolor producto del movimiento dental, en la reparación ósea después de la expansión, en diversas cirugías y otras aplicaciones ...

  1. Single Bacterium Detection Using Sers

    Science.gov (United States)

    Gonchukov, S. A.; Baikova, T. V.; Alushin, M. V.; Svistunova, T. S.; Minaeva, S. A.; Ionin, A. A.; Kudryashov, S. I.; Saraeva, I. N.; Zayarny, D. A.

    2016-02-01

    This work is devoted to the study of a single Staphylococcus aureus bacterium detection using surface-enhanced Raman spectroscopy (SERS) and resonant Raman spectroscopy (RS). It was shown that SERS allows increasing sensitivity of predominantly low frequency lines connected with the vibrations of Amide, Proteins and DNA. At the same time the lines of carotenoids inherent to this kind of bacterium are well-detected due to the resonance Raman scattering mechanism. The reproducibility and stability of Raman spectra strongly depend on the characteristics of nanostructured substrate, and molecular structure and size of the tested biological object.

  2. 基于金纳米聚集体的核壳型SERS探针及其细胞内应用%Preparation of Silica-coated Aggregated Gold Nanoparticles and Their SERS Performance in Living Cells

    Institute of Scientific and Technical Information of China (English)

    杨晶; 王著元; 张若虎; 宋春元; 李锦; 高文成; 崔一平

    2011-01-01

    利用金纳米粒子的聚集体作为表面增强拉曼散射(Surface enhanced Raman scattering,SERS)的增强基底,合成了一种二氧化硅包裹的核壳型SERS探针,并成功将该探针应用于活细胞的SERS光谱探测.实验中利用4-巯基苯甲酸(4-mercaptobenzoicacid,4MBA)作为拉曼标记物,并讨论了其在诱导金纳米粒子的聚集过程中的作用.通过包二氧化硅外壳来实现对金纳米颗粒聚集程度的控制,保持并优化了该探针的SERS活性.同时,通过在金纳米颗粒外包裹一层二氧化硅外壳,该SERS探针的化学稳定性和生物兼容性得到了很大的提高.利用紫外-可见吸收光谱仪以及透射电子显微镜研究了该探针中金纳米颗粒的聚集程度以及核壳结构的形貌.实验结果表明该探针在活细胞内具有很好的SERS灵敏度,并且生物兼容性好,可以进一步用于细胞内生理活动监测.%We present a novel core/shell SERS(surface enhanced Raman scattering) probe based on silica-coated gold nanoaggregates and its SERS application in living cells.It is found that the chemical stability and biocompatibility can be greatly improved by coating the gold nanoparticles with a layer of silica.In our experiments,4MBA(4-mercaptobenzoicacid) is used not only as a Raman reporter,but also an aggregation agent.By coating the gold nanoparticles with the silica shell,the aggregation of gold nanoparticles can be controlled to optimize the SERS activity.TEM images and UV-Vis spectra were em-ployed to investigate the core-shell structure of the probe and the aggregation degree of gold nanoparticles.The results exhibit the strong performance of SERS sensitivity and biocompatibility of the probe in living HeLa and SKBR3 cells,indicating its attraction in further application in intracellular environment.

  3. Upconversion luminescence properties of Y2O2S:Er3+@Y2O2S:Yb3+,Tm3+ core-shell nanoparticles prepared via homogeneous co-precipitation

    Science.gov (United States)

    Tian, Ying; Lu, Fei; Xing, Mingming; Ran, Jincheng; Fu, Yao; Peng, Yong; Luo, Xixian

    2017-02-01

    The Y2O2S:Er3+@Y2O2S:Yb3+,Tm3+ core-shell upconversion (UC) nanoparticles with average diameter of 95 nm were synthesized by the homogeneous co-precipitation method combining with the solid-gas sulfidation route. The increases of nanocrystaline size after the shell coating was observed both in the X-ray diffraction (XRD) and transmission electron microscope (TEM) measurements. This indicates the composition homogeneity core-shell Y2O2S nanocrystals. Meanwhile, the luminescence of both the Er3+ and Tm3+ ions are realized for the first time in the novel core-shell Y2O2S:Er3+@Y2O2S:Yb3+,Tm3+ nanoparticles under the excitations of both 980 and 1550 nm. When excited by a 980 nm laser diode, the Y2O2S:Er3+@Y2O2S:Yb3+,Tm3+ phosphor exhibits blue (≈475 nm), green (≈548 nm) and red (≈670 nm) emissions in the visible region, which correspond to the 1G4 → 3H6 transition of Tm3+ions, 4S3/2, 2H11/2 → 4I15/2 and 4F9/2 → 4I15/2 transitions of Er3+ ions, respectively. The very strong emission at the near infrared (NIR) region is mainly due to the 3H4 → 3H6 transition of Tm3+ ions. The emission from both of Er3+ and Tm3+ ions under 980 nm excitation reveals the energy transfers of Yb3+ → Tm3+ within the shell layer and Yb3+ → Er3+ between the shell and the core. When pumping at 1550 nm, although only Er3+ ions can efficiently absorb the excitation energy, the strong UC emissions from Tm3+ ions were also observed. This is owing to the energy transfer between the core and the shell through Er3+ → Yb3+ → Tm3+ ions.

  4. SERS sensors for DVD platform

    DEFF Research Database (Denmark)

    Brøgger, Anna Line

    This Ph.D. thesis explores the engineering of a portable sensor system for detection of rare and small molecules. The Ph.D. project is part of the research project 'Multi-Sensor DVD platform' (MUSE), aiming to integrate different sensors on a rotating disc. The sensors are chosen to complement each...... other, creating more reliable and stable results for the end user. The rotating disc comprises microfluidic channels, which can be utilized for handling and manipulating liquid samples such as blood or water. The focus of this Ph.D. thesis, is on the integration of one specific sensor on a rotating disc....... The sensor is based upon surface enhanced Raman spectroscopy (SERS), which detects molecular vibrations. The aim of this thesis is to cover the different aspects of the sensor system. SERS substrates, consisting of nanopillars with gold or silver caps on top, have been fabricated by standard micro and nano...

  5. 修饰银纳米粒子的石墨烯泡沫镍衬底制备及其SERS活性研究%Preparation of Silver Nanoparticles Decorated Graphene Based Nickel Foam Substrate for SERS Activity Study

    Institute of Scientific and Technical Information of China (English)

    谷峰; 刘小红; 何石轩; 蒲利春; 魏大鹏

    2015-01-01

    为了解决表面增强拉曼散射(SERS)衬底的吸附性差、稳定性低以及灵敏度不高的问题,设计了一种沉积银纳米粒子的石墨烯泡沫镍SERS衬底,并进行了实验研究.利用化学气相沉积法在泡沫镍衬底上生长石墨烯,并通过溶液沉积的方法将合成的银纳米粒子沉积在石墨烯泡沫镍衬底表面,烘干后制备成石墨烯泡沫镍修饰银纳米粒子的新型SERS衬底.采用罗丹明6G(R6G)对SERS衬底进行拉曼实验研究,结果表明石墨烯能够较好地淬灭SERS衬底的背景荧光;泡沫镍的独特三维结构能够增大衬底对检测分子的吸附;同时,银纳米粒子也可大幅增强衬底的SERS活性.而修饰了银纳米粒子的石墨烯泡沫镍新型衬底同时具有以上优异特性,是一种具有很大应用潜力的新型SERS衬底.

  6. El hombre, ser de palabra

    OpenAIRE

    Presas, Mario A.

    2006-01-01

    RESUMEN: Paul Ricoeur distingue la mismidad del carácter (idem), de la ipseidad, el ser que se mantiene en la promesa, el maintien de soi, que encuentra su más fiel expresión en las variaciones imaginarias de la ficción literaria. El presente trabajo analiza obras no tenidas en cuenta por Ricoeur, como Kaspar de Peter Handke, para ejemplificar la pérdida de la ipseidad si no hay un juego dialéctico con un carácter, caso que también se muestra en Der Mann ohne Eigenschaften de Musil. Un "sueño...

  7. Ser poeta: poemas al llano

    OpenAIRE

    1999-01-01

    "Ser poeta es viajar sobre mares revueltos y soltar las amarras sin pensar en el puerto" El alma de un poeta como Getulio, viajará eternamente solitaria por los caminos del llano. Sus versos no morirán jamás. En la medida en que pase el tiempo se tornarán en el alma del folklore llanero y los repetirá el eco solitario de las sabanas en las noches de luna. Getulio lleva el verso a flor de labios, su alma es poesía. Le parece que ve crecer las plantas, abrirse las flores en las mañanas,...

  8. SERS biodetection using gold-silica nanoshells and nitrocellulose membranes.

    Science.gov (United States)

    Bishnoi, Sandra Whaley; Lin, Yu-jen; Tibudan, Martin; Huang, Yiming; Nakaema, Marcelo; Swarup, Vimal; Keiderling, Timothy A

    2011-06-01

    We have developed a rapid, reproducible, easy to execute, surface enhanced Raman scattering (SERS) method for detection of low volumes and total amounts of biological antigens using an analyte capture system derived from methods commonly used in Western blotting. Our method is a "half-sandwich" assay with an antigen detection scheme that employs a nitrocellulose (NC) membrane with 200 nm pore size to capture subnanograms of analyte and concentrate them in a small area from applied volumes as low as one microliter. The SERS probes used for detection consist of gold-silica nanoshells modified with a two-component mixed monolayer system. One component consists of a poly(ethylene glycol) (PEG)-modified Raman-active chromophore bound to the gold surface which allows for SERS detection and imparts particle stability. The second component uses (ortho-pyridyl) disulfide-PEG-succinimidyl ester to couple the recognition antibody to the particle surface. By controlling the reaction time and concentration of thiols, a mixed monolayer is prepared on the nanoshell surface with the ability to recognize low concentrations of analyte and generate reproducible SERS signals. Using this strategy, we have achieved SERS signals that are proportional to antigen present on the membrane allowing detection of total antigen amounts as low as 1.25 ng for some cases. The performance of this new SERS bioassay has been tested with a variety of potential antigens, demonstrating the potential for multiplexed detection of analytes.

  9. Surface enhanced Raman scattering (SERS) fabrics for trace analysis

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Jun [National Engineering Laboratory for Advanced Yarn and Fabric Formation and Clean Production, Wuhan Textile University, Wuhan 430073 (China); Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Ministry of Education & College of Chemistry & Chemical Engineering, Hubei University, Wuhan 430062 (China); Zhou, Ji [Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Ministry of Education & College of Chemistry & Chemical Engineering, Hubei University, Wuhan 430062 (China); Tang, Bin, E-mail: bin.tang@deakin.edu.au [National Engineering Laboratory for Advanced Yarn and Fabric Formation and Clean Production, Wuhan Textile University, Wuhan 430073 (China); Institute for Frontier Materials, Deakin University, Geelong, Victoria 3216 (Australia); Zeng, Tian; Li, Yaling [Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Ministry of Education & College of Chemistry & Chemical Engineering, Hubei University, Wuhan 430062 (China); Li, Jingliang [Institute for Frontier Materials, Deakin University, Geelong, Victoria 3216 (Australia); Ye, Yong, E-mail: yeyong@hubu.edu.cn [Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Ministry of Education & College of Chemistry & Chemical Engineering, Hubei University, Wuhan 430062 (China); Wang, Xungai [National Engineering Laboratory for Advanced Yarn and Fabric Formation and Clean Production, Wuhan Textile University, Wuhan 430073 (China); Institute for Frontier Materials, Deakin University, Geelong, Victoria 3216 (Australia)

    2016-11-15

    Highlights: • Gold nanoparticles are in-situ synthesized on silk fabrics by heating. • Flexible silk fabrics with gold nanoparticles are used for surface-enhanced Raman scattering (SERS). • SERS activities of silk fabrics with different gold contents are investigated. - Abstract: Flexible SERS active substrates were prepared by modification of silk fabrics with gold nanoparticles. Gold nanoparticles were in-situ synthesized after heating the silk fabrics immersed in gold ion solution. Localized surface plasmon resonance (LSPR) properties of the treated silk fabrics varied as the concentration of gold ions changed, in relation to the morphologies of gold nanoparticles on silk. In addition, X-ray diffraction (XRD) was used to observe the structure of the gold nanoparticle treated silk fabrics. The SERS enhancement effect of the silk fabrics treated with gold nanoparticles was evaluated by collecting Raman signals of different concentrations of p-aminothiophenol (PATP), 4-mercaptopyridine (4-MPy) and crystal violet (CV) solutions. The results demonstrate that the silk fabrics corresponding to 0.3 and 0.4 mM of gold ions possess high SERS activity compared to the other treated fabrics. It is suggested that both the gold content and morphologies of gold nanoparticles dominate the SERS effect of the treated silk fabrics.

  10. por láser

    Directory of Open Access Journals (Sweden)

    Mayra Garcimuño

    2013-01-01

    Full Text Available En el presente trabajo, la técnica Espectroscopia de plasmas producidos por láser (Laser-induced breakdown spectroscopy –LIBS– se aplicó a la determinación cuan- titativa de Na en agua natural dulce, de interés en agricultura para el estudio de la alcalinidad de aguas de regadío. Para efectuar el análisis, se prepararon soluciones con concentraciones conocidas del analito, se mezclaron con óxido de calcio y se compactaron en pastillas sólidas. Los plasmas se produjeron en aire a presión atmos- férica utilizando un láser pulsado Nd:YAG. Se construyó una curva de calibración y se calculó el límite de detección. Se analizaron muestras de agua natural y los resultados se compararon con los obtenidos mediante espectroscopia de absorción atómica. Se demostró la factibilidad del método para la determinación de Na en agua natural dulce.

  11. Microfluidic setup for on-line SERS monitoring using laser induced nanoparticle spots as SERS active substrate

    Directory of Open Access Journals (Sweden)

    Oana-M. Buja

    2017-01-01

    Full Text Available A microfluidic setup which enables on-line monitoring of residues of malachite green (MG using surface-enhanced Raman scattering (SERS is reported. The SERS active substrate was prepared via laser induced synthesis of silver or gold nanoparticles spot on the bottom of a 200 μm inner dimension glass capillary, by focusing the laser beam during a continuous flow of a mixture of silver nitrate or gold chloride and sodium citrate. The described microfluidic setup enables within a few minutes the monitoring of several processes: the synthesis of the SERS active spot, MG adsorption to the metal surface, detection of the analyte when saturation of the SERS signal is reached, and finally, the desorption of MG from the spot. Moreover, after MG complete desorption, the regeneration of the SERS active spot was achieved. The detection of MG was possible down to 10−7 M concentration with a good reproducibility when using silver or gold spots as SERS substrate.

  12. New nanocomposites for SERS studies of living cells and mitochondria

    DEFF Research Database (Denmark)

    Sarycheva, A. S.; Brazhe, N. A.; Baizhumanov, A. A.

    2016-01-01

    molecules. The SERS spectra of functional mitochondria are sensitive to the activity of the mitochondrial electron transport chain, thus making the method a novel label-free approach to monitor the redox state and conformation of cytochromes in their natural cell environment. The developed nanocomposites......A great enhancement in Raman scattering (SERS) from heme-containing submembrane biomolecules inside intact erythrocytes and functional mitochondria is demonstrated for the first time using silver–silica beads prepared using a new method involving aerosol pyrolysis with aqueous diamminesilver...

  13. Flexible SERS Substrates: Challenges and Opportunities

    Science.gov (United States)

    2016-01-28

    multifunctionality to SERS substrates to achieve separation, preconcentration and detection on flexible SERS substrates. Clearly, progress along this...developed a microfluidic device that detects trace concentrations of drugs of abuse in saliva within minutes using SERS . Its operation was demonstrated...substrates without hot spots are demonstrated with SERS enhancements near 108 compared to non-enhancing substrates for the detection of benzenethiol. The

  14. El Hombre, un ser integral

    Directory of Open Access Journals (Sweden)

    Gabriel Alexander Solórzano

    2008-12-01

    Full Text Available El artículo pretende mostrar la dificultad que existe al momento de elaborar una precisa definición del hombre como ser integral, para ello se recurre a las nociones esenciales de la antropología filosófica, la nueva concepción del giro cristia- no de la fenomenología francesa, se mira grosso modo la visión personalista de Juan Pablo II sobre el hombre y la visión integradora de Panikkar del humanismo cosmote- ándrico, por último se encuentra la visión del humanismo cristiano y el desarrollo soli- dario enfatizado en el Magisterio de la Iglesia Católica como intento de respuesta a la crucial pregunta sobre el hombre y su mundo relacional. Palabras clave: Filosofía, Teología moral, Fenomenología, Humanismo, Perso- nalismo, Cosmoteándrico. 

  15. Rough surface Au@Ag core-shell nanoparticles to fabricating high sensitivity SERS immunochromatographic sensors.

    Science.gov (United States)

    Fu, Qiangqiang; Liu, Hongwu Liu; Wu, Ze; Liu, An; Yao, Cuize; Li, Xiuqing; Xiao, Wei; Yu, Shiting; Luo, Zhi; Tang, Yong

    2015-11-14

    Immunochromatographic sensors (ICSs) are inexpensive, simple, portable, and robust, thus making ICSs commonplace in clinical diagnoses, food testing, and environmental monitoring. However, commonly used gold nanoparticles (AuNPs) ICSs have low sensitivity. Therefore, we developed highly sensitive surface enhanced Raman scattering (SERS) ICSs. To enhance the sensitivity of SERS ICSs, rough surface core-shell Au@Ag nanoparticles (RSAu@AgNPs) were prepared by coating silver on the surface of gold nanoflowers (AuNFs). Then these nanoparticles were used as SERS substrate in the SERS ICSs, after which the SERS ICSs were implemented to detect haemoglobin and heavy metal cadmium ion (Cd(2+)). The limit of detection (LOD) of the SERS ICSs for detecting haemoglobin was 8 ng/mL, and the linear range of the SERS ICSs was from 31.3 to 2000 ng/mL. The LOD of the SERS ICSs for detecting Cd(2+) was 0.05 ng/mL and the linear analysis range was from 0.05 to 25 ng/mL. The cross reactivity of the SERS ICSs was studied and results showed that the SERS ICSs exhibited highly specific for detection of haemoglobin and Cd(2+), respectively. The SERS ICSs were then used to detect haemoglobin (spiked in serum and in stool) and Cd(2+) (spiked in tap water, river water, and soil leaching water), and the results showed high recovery. These characteristics indicated that SERS ICSs were ideal tools for clinical diagnosis and environmental pollution monitoring.

  16. Double Detection of Mycotoxins Based on SERS Labels Embedded Ag@Au Core-Shell Nanoparticles.

    Science.gov (United States)

    Zhao, Yuan; Yang, Yaxin; Luo, Yaodong; Yang, Xuan; Li, Manli; Song, Qijun

    2015-10-01

    A sensitive surface-enhanced Raman scattering (SERS) signal dependent double detection of mycotoxins is achieved for the first time, without the aid of nucleic acid amplification strategies. SERS labels embedded Ag@Au core-shell (CS) nanoparticles (NPs) as novel SERS tags are successfully prepared through a galvanic replacement-free deposition. SERS tags produce stable and quantitative SERS signal, emerging from the plasmonic coupling at the junction of Ag core and Au shell. SERS tags engineered Raman aptasensors are developed for the double detection of ochratoxin A (OTA) and aflatoxin B1 (AFB1) in maize meal. The limits of detection (LODs) are as low as 0.006 ng/mL for OTA and 0.03 ng/mL for AFB1. The developed protocol can be extended to a large set of different SERS tags for the sensitive detection of multiple targets that possess different lengths of aptamers.

  17. SERS-based application in food analytics (Conference Presentation)

    Science.gov (United States)

    Cialla-May, Dana; Radu, Andreea; Jahn, Martin; Weber, Karina; Popp, Jürgen

    2017-02-01

    To establish detection schemes in life science applications, specific and sensitive methods allowing for fast detection times are required. Due to the interaction of molecules with strong electromagnetic fields excited at metallic nanostructures, the molecular fingerprint specific Raman spectrum is increased by several orders of magnitude. This effect is described as surface-enhanced Raman spectroscopy (SERS) and became a very powerful analytical tool in many fields of application. Within this presentation, we will introduce innovative bottom-up strategies to prepare SERS-active nanostructures coated with a lipophilic sensor layer. To do so, the food colorant Sudan III, an indirect carcinogen substance found in chili powder, palm oil or spice mixtures, is detected quantitatively in the background of the competitor riboflavin as well as paprika powder extracts. The SERS-based detection of azorubine (E122) in commercial available beverages with different complexity (e.g. sugar content, alcohol concentration) illustrates the strong potential of SERS as a qualitative as well as semiquantitative prescan method in food analytics. Here, a good agreement between the estimated concentration employing SERS as well as the gold standard technique HPLC, a highly laborious method, is found. Finally, SERS is applied to detect vitamin B2 and B12 in cereals as well as the estimate the ratio of lycopene and β-carotene in tomatoes. Acknowledgement: Funding the projects "QuantiSERS" and "Jenaer Biochip Initiative 2.0" within the framework "InnoProfile Transfer - Unternehmen Region" the Federal Ministry of Education and Research, Germany (BMBF) is gratefully acknowledged.

  18. Ser marrano em Minas Colonial

    Directory of Open Access Journals (Sweden)

    Anita Novinsky

    2001-01-01

    Full Text Available Baseado em 57 Processos inéditos de marranos (cristãos-novos, conversos presos em Minas Gerais no século XVIII, este artigo procura mostrar seu papel na Idade de Ouro do Brasil. 64% deles eram mercadores e 23% eram mineiros. Pertenciam à classe média e raros eram os magnatas. Nenhum dele esteve envolvido no tráfico negreiro. Acusados do crime de judaísmo e de pertencerem a sociedades secretas, representaram 42% dos brasileiros condenados à morte. Ser marrano entre os portugueses no Brasil mais um sentimento e uma visão de mundo do que uma prática religiosa.Based on 57 unknown trials of marranos (new christians, conversos arrested by the Inquisition in Minas Gerais in the XVIII the century, this article shows their role in shapping the history of Minas Gerais. 64% of them were merchants na 23% were miners. Most of them belonged to a middle class and rare were those whit great fortunes. No one of them were engaged in slave trade. Acused of judaism and of participating in secret society the marranos of Minas represented 42% of the Brazilian burned at the stake. Being a marrano in Minas Gerais was not always being a crypto-new. Marranism in Brasil was a feeling and a approach to life.

  19. Au-ZnO hybrid nanoparticles exhibiting strong charge-transfer-induced SERS for recyclable SERS-active substrates

    Science.gov (United States)

    Liu, Liping; Yang, Haitao; Ren, Xiao; Tang, Jin; Li, Yongfeng; Zhang, Xiangqun; Cheng, Zhaohua

    2015-03-01

    Flower-shaped Au-ZnO hybrid nanoparticles have been prepared via seeding growth and subsequent wet-chemical etching of Au-ZnO core-shell nanoparticles. The etched Au-ZnO hybrid nanoparticles have shown a stronger surface-enhanced Raman scattering (SERS) signal of the nontotally symmetric (b2) vibrational modes of PATP molecules than Au nanoparticles alone, which is attributed to the chemical enhancement effect of the ZnO layer which is greatly excited by the localized surface plasmon resonance (LSPR) of Au cores. Further, the mechanism of the LSPR-enhanced charge transfer (CT) effect has been proved by the SERS spectra of PATP molecules excited using different laser sources from 325 to 785 nm. Moreover, the photocatalytic experimental results indicated that Au-ZnO hybrid nanoparticles are promising as biologically compatible and recyclable SERS-active platforms for different molecular species.Flower-shaped Au-ZnO hybrid nanoparticles have been prepared via seeding growth and subsequent wet-chemical etching of Au-ZnO core-shell nanoparticles. The etched Au-ZnO hybrid nanoparticles have shown a stronger surface-enhanced Raman scattering (SERS) signal of the nontotally symmetric (b2) vibrational modes of PATP molecules than Au nanoparticles alone, which is attributed to the chemical enhancement effect of the ZnO layer which is greatly excited by the localized surface plasmon resonance (LSPR) of Au cores. Further, the mechanism of the LSPR-enhanced charge transfer (CT) effect has been proved by the SERS spectra of PATP molecules excited using different laser sources from 325 to 785 nm. Moreover, the photocatalytic experimental results indicated that Au-ZnO hybrid nanoparticles are promising as biologically compatible and recyclable SERS-active platforms for different molecular species. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr00491h

  20. Ciprofloxacin: pH-dependent SERS signal and its detection in spiked river water using LoC-SERS.

    Science.gov (United States)

    Hidi, Izabella J; Heidler, Jan; Weber, Karina; Cialla-May, Dana; Popp, Jürgen

    2016-11-01

    Monitoring the successful removal of antibiotics in waste and surface waters is of high interest to overcome the occurrence of antibacterial resistance in the ecosystem. Among the newly developed analytical methods, the lab-on-a-chip surface-enhanced Raman spectroscopic (LoC-SERS) technique has gained the interest of the scientific community in the last few years. Ciprofloxacin, a second-generation fluoroquinolone, is widely used and administered to patients in dosages up to 1000 mg. In addition, more than 50 % of the antibiotic is excreted in urine as the parental drug. Thus, ciprofloxacin in environmental samples may exceed the minimum inhibitory concentration (MIC) values. The present study aims to assess the potential of the LoC-SERS technique to detect the target analyte in spiked river water samples at MIC concentrations. As sample clean-up procedure, a simple filtration is proposed, while as SERS, active substrates silver nanoparticles prepared at room temperature are employed. Ciprofloxacin was successfully quantified in the 0.7-10 μM concentration range with data that were measured on two different days. Furthermore, because of the low solubility of the antibiotic at the neutral pH range, insights into the effect of pH on the SERS signal of the target molecule are also presented. Graphical Abstract Ciprofloxacin detected at MIC values by LOC-SERS.

  1. Patchy silica-coated silver nanowires as SERS substrates

    Energy Technology Data Exchange (ETDEWEB)

    Hunyadi Murph, Simona E.; Murphy, Catherine J.

    2013-05-08

    We report a class of core-shell nanomaterials that can be used as efficient surface-enhancement Raman scattering (SERS) substrates. The core consists of silver nanowires, prepared through a chemical reduction process, that are used to capture 4- mercaptobenzoic acid (4-MBA), a model analyte. The shell was prepared through a modified Stöber method and consists of patchy or full silica coats. The formation of silica coats was monitored via transmission electron microscopy, UV-visible spectroscopy and phase-analysis light scattering for measuring effective surface charge. Surprisingly, the patchy silica coated silver nanowires are better SERS substrate than silver nanowires; nanomolar concentration of 4-MBA can be detected. In addition, “nano-matryoshka” configurations were used to quantitate/explore the effect of the electromagnetic field at the tips of the nanowire (“hot spots”) in the Raman scattering experiment.

  2. SERS and RAIR for Molecular Structure of Phenylazonaphthalene terminated Self-Assembled Monolayer

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The SAMs of 10-[4-(4-phenylazo)naphth-l-oxyl]-l-decanethiol (Compound 1) on gold were prepared. Their molecular structures were determined by surface enhanced Raman spectroscopy (SERS) and reflection absorption infrared spectroscopy (RAIR).

  3. Design and Preparation of Nanoparticle Dimers for SERS Detection

    Science.gov (United States)

    2012-09-10

    into the Synthesis, Design and Processing of Narrow Band Gap Organic Semiconducting Polymers for Solar Cell Fabrication, Unam National Institute of...Technology, Unam , Korea, November 2010. Insight into the Synthesis, Design and Processing of Narrow Band Gap Organic Semiconducting Polymers for Solar

  4. Standard method for characterizing SERS substrates

    Science.gov (United States)

    Guicheteau, Jason A.; Hankus, Mikella E.; Christesen, Steven D.; Fountain, Augustus W., III; Pellegrino, Paul M.; Emmons, Erik D.; Tripathi, Ashish; Wilcox, Phillip; Emge, Darren

    2012-06-01

    We present the methodology and results of a standard assessment protocol to evaluate disparate SERS substrates that were developed for the Defense Advanced Research Programs Agency (DARPA) SERS Science and Technology Fundamentals Program. The results presented are a snapshot of a collaborative effort between the US Army Edgewood Chemical Biological Center, and the US Army Research Laboratory-Aldelphi Laboratory Center to develop a quantitative analytical method with spectroscopic figures of merit to unambiguously compare the sensitivity and reproducibility of various SERS substrates submitted by the program participants. We present the design of a common assessment protocol and the definition of a SERS enhancement value (SEV) in order to effectively compare SERS active surfaces.

  5. 16 CFR 1018.42 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Annual report. 1018.42 Section 1018.42... Reports and Audits § 1018.42 Annual report. (a) The Advisory Committee Management Officer shall prepare an annual report on the Commission's advisory committees for inclusion in the President's annual report...

  6. Review of SERS Substrates for Chemical Sensing

    Directory of Open Access Journals (Sweden)

    Pamela A. Mosier-Boss

    2017-06-01

    Full Text Available The SERS effect was initially discovered in the 1970s. Early research focused on understanding the phenomenon and increasing enhancement to achieve single molecule detection. From the mid-1980s to early 1990s, research started to move away from obtaining a fundamental understanding of the phenomenon to the exploration of analytical applications. At the same time, significant developments occurred in the field of photonics that led to the advent of inexpensive, robust, compact, field-deployable Raman systems. The 1990s also saw rapid development in nanoscience. This convergence of technologies (photonics and nanoscience has led to accelerated development of SERS substrates to detect a wide range of chemical and biological analytes. It would be a monumental task to discuss all the different kinds of SERS substrates that have been explored. Likewise, it would be impossible to discuss the use of SERS for both chemical and biological detection. Instead, a review of the most common metallic (Ag, Cu, and Au SERS substrates for chemical detection only is discussed, as well as SERS substrates that are commercially available. Other issues with SERS for chemical detection have been selectivity, reversibility, and reusability of the substrates. How these issues have been addressed is also discussed in this review.

  7. Shape-controllable gold nanostructures and their SERS enhancement

    Science.gov (United States)

    Ma, Ying; Promthaveepong, Kittithat; Li, Nan

    2016-10-01

    We report a new growth mechanism for the formation of gold (Au) nanoflowers (NFs) or Au nanoplates by simply tuning the solvent compositions. An aniline derivative N-(3-amidino)-aniline (NAAN) can reduce HAuCl4 to form AuNFs in water, while individual Au microplates were prepared in N-methyl-pyrrolidone (NMP)/water mixed solvents. Growth mechanism showed that incomplete coverage of Au (111) facets by oxidized product poly (N-(3-amidino)-aniline) (PNAAN) resulted in AuNFs, and improved coverage on Au (111) facet by NMP was favorable for individual Au microplate formation. The layered AuNFs showed 11 times SERS enhancement compared with randomly packed Au microplate film. As low as 10‑12 M 4-mercaptobenzoic acid (4-MBA) can be detected using AuNF as a SERS substrate.

  8. SERS activity of self-cleaning silver/titania nanoarray

    Energy Technology Data Exchange (ETDEWEB)

    Xie, Yibing, E-mail: ybxie@seu.edu.cn [School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189 (China); Suzhou Research Institute of Southeast University, Suzhou 215123 (China); Jin, Yanyan [School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189 (China); Suzhou Research Institute of Southeast University, Suzhou 215123 (China); Zhou, Yingzhi; Wang, Yong [School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189 (China)

    2014-09-15

    Highlights: • Ag/TiO{sub 2} nanoarray is prepared by depositing Ag on TiO{sub 2} nanorod and nanotube array. • Ag/TiO{sub 2} nanoarray shows good SERS activity and self-cleaning performance as well. • Ag/TiO{sub 2} NRA as SERS substrate shows a low detection limit of PMBA below 5 × 10{sup −12} M. - Abstract: Silver decorated titania nanorod array (Ag/TiO{sub 2} NRA) and nanopore array (Ag/TiO{sub 2} NPA) had been designed as surface-enhanced Raman scattering (SERS) active substrate for a sensitive detection application. TiO{sub 2} NRA grown on glass slide was fabricated through hydrothermal synthesis process. TiO{sub 2} NPA grown on the titanium sheet was fabricated by a two-stepped anodization process. Ag/TiO{sub 2} NRA and Ag/TiO{sub 2} NPA were formed by depositing silver nanoparticles on TiO{sub 2} nanoarray through an electroless deposition process. Ag/TiO{sub 2} NRA had smaller silver interparticle gaps and more hot-spots than Ag/TiO{sub 2} NPA, exhibiting a higher SERS activity. The analytical enhancement factor was accordingly increased from 1.8 × 10{sup 5} for Ag/TiO{sub 2} NPA up to 7.8 × 10{sup 5} for Ag/TiO{sub 2} NRA. Ag/TiO{sub 2} NRA substrate achieved a very low detection limit of 5 × 10{sup −12} M 4-mercaptobenzoic acid as a probe molecule, showing a sensitive SERS detection performance. Ag/TiO{sub 2} NRA also conducted photocatalysis decomposition of these adsorbed organic molecules to recover a clean surface under UV irradiation, exhibiting a self-cleaning activity. Such an Ag/TiO{sub 2} nanoarray with well-controlled size and shape could be suitably applies as a self-cleaning SERS substrate, showing high reproducibility and cycleability in recycling SERS detection application.

  9. O efeito SERS na análise de traços: o papel das superfícies nanoestruturadas The SERS effect in trace analysis: the role of nanostructured surfaces

    Directory of Open Access Journals (Sweden)

    Antonio C. Sant'Ana

    2006-07-01

    Full Text Available Surface-Enhanced Raman Scattering - SERS - underwent huge advances since a single-molecule Raman spectrum was obtained in 1997. New theoretical and experimental approaches emerged since then leading to a better understanding of the enhancement mechanisms and to a significant improvement in the Raman signal. This review presents the current status of the SERS effect and the promising ways of designing and preparing high performance SERS-active substrates.

  10. Conocer y ser en el paradigma constructivista

    Directory of Open Access Journals (Sweden)

    Jose Antonio Camargo Rodriguez

    2014-03-01

    Full Text Available Toda teoría acerca del aprendizaje se fundamenta en una interpretación del conocimiento, la cual se encuentra, a su vez, ligada a una cierta concepción de «ser». No será posible asimilar verdaderamente cualquiera de tales teorías si se ignoran, o no se consideran con el debido detenimiento, las ideas de conocer y «ser» que le sirven de base. Sc pone de presente que el constructivismo, en contraste con la teoría transmisionista de la enseñanza y el aprendizaje, predominante en la pedagogía tradicional, tiene su fundamento en la interpretación según la cual el conocer es una actividad humana en la que, a medida quo conoce, el hombre construye el «ser». Antes de todo conocimiento, las cosas no tienen un «ser»; están ahí, pero no se sabe lo que son. El «ser», quo constituye el objeto de todo conocer, aquello que el sujeto persigue a través de su conocimiento, no toes dada de antemano, ni le viene de fuera, sino quo es una elaboración quo el mismo realiza a través de su actividad cognoscitiva, un contenido de su propia conciencia. Hay, pues, una cierta paradoja entre las ideas de conocer y «ser» que sirven de fundamento al constructivismo, cuya reflexión se propone en aras de ganar una mejor comprensión, de encontrarle a este paradigma un sentido más allá de la pedagogía y la didáctica.

  11. Long-term stable silver subsurface ion-exchanged glasses for SERS applications.

    Science.gov (United States)

    Simo, Anne; Joseph, Virginia; Fenger, Robert; Kneipp, Janina; Rademann, Klaus

    2011-06-20

    We report on the formation of silver subsurface ion-exchanged metal oxide (silver SIMO) glasses and their surface-enhanced Raman scattering (SERS) activity. The samples were prepared by a combined thermal and chemical three-step methodology and characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), environmental electron scanning microscopy (ESEM), and UV/Vis spectroscopy. This unique method provides SERS substrates with protection against contamination and strong, reliable and reproducible SERS enhancement. The Raman enhancement factors of the long-term stable SIMO glasses were estimated to approximately 10(7).

  12. International Energy Annual, 1992

    Energy Technology Data Exchange (ETDEWEB)

    1994-01-14

    This report is prepared annually and presents the latest information and trends on world energy production and consumption for petroleum, natural gas, coal, and electricity. Trade and reserves are shown for petroleum, natural gas, and coal. Prices are included for selected petroleum products. Production and consumption data are reported in standard units as well as British thermal units (Btu) and joules.

  13. LLNL NESHAPs 2004 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Harrach, R; Gallegos, G; Peterson, R; Wilson, K; Harrach, R J; Gallegos, G M; Peterson, S R; Wilson, K R

    2005-06-27

    This annual report is prepared pursuant to the National Emission Standards for Hazardous Air Pollutants (NESHAPs; Title 40 Code of Federal Regulations [CFR] Part 61, Subpart H). Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities.

  14. The detection of dust around NN Ser

    Science.gov (United States)

    Hardy, Adam; Schreiber, Matthias R.; Parsons, Steven G.; Caceres, Claudio; Brinkworth, Carolyn; Veras, Dimitri; Gänsicke, Boris T.; Marsh, Thomas R.; Cieza, Lucas

    2016-07-01

    Eclipse timing variations observed from the post-common-envelope binary (PCEB) NN Ser offer strong evidence in favour of circumbinary planets existing around PCEBs. If real, these planets may be accompanied by a disc of dust. We here present the ALMA detection of flux at 1.3 mm from NN Ser, which is likely due to thermal emission from a dust disc of mass ˜0.8 ± 0.2 M⊕. We performed simulations of the history of NN Ser to determine possible origins of this dust, and conclude that the most likely origin is, in fact, common-envelope material which was not expelled from the system and instead formed a circumbinary disc. These discs have been predicted by theory but previously remained undetected. While the presence of this dust does not prove the existence of planets around NN Ser, it adds credibility to the possibility of planets forming from common-envelope material in a `second-generation' scenario.

  15. Hur ser egentligen AKK-samspel ut?

    DEFF Research Database (Denmark)

    Pilesjö, Maja Sigurd

    Hur ser egentligen AKK-samspel ut? Interaktionsmönster mellan barn med omfattande kommunikativ och motorisk funktionsnedsättning och deras vardagliga samtalspartners. Hur kan egentligen samspel mellan barn med kommunikationsvårighe- ter gå till och faktiskt också fung- era? Maja Sigurd Pilesjö...

  16. Plasmonic SERS biosensing nanochips for DNA detection.

    Science.gov (United States)

    Ngo, Hoan T; Wang, Hsin-Neng; Fales, Andrew M; Vo-Dinh, Tuan

    2016-03-01

    The development of rapid, cost-effective DNA detection methods for molecular diagnostics at the point-of-care (POC) has been receiving increasing interest. This article reviews several DNA detection techniques based on plasmonic-active nanochip platforms developed in our laboratory over the last 5 years, including the molecular sentinel-on-chip (MSC), the multiplex MSC, and the inverse molecular sentinel-on-chip (iMS-on-Chip). DNA probes were used as the recognition elements, and surface-enhanced Raman scattering (SERS) was used as the signal detection method. Sensing mechanisms were based on hybridization of target sequences and DNA probes, resulting in a distance change between SERS reporters and the nanochip's plasmonic-active surface. As the field intensity of the surface plasmon decays exponentially as a function of distance, the distance change in turn affects SERS signal intensity, thus indicating the presence and capture of the target sequences. Our techniques were single-step DNA detection techniques. Target sequences were detected by simple delivery of sample solutions onto DNA probe-functionalized nanochips and measuring the SERS signal after appropriate incubation times. Target sequence labeling or washing to remove unreacted components was not required, making the techniques simple, easy-to-use, and cost-effective. The usefulness of the nanochip platform-based techniques for medical diagnostics was illustrated by the detection of host genetic biomarkers for respiratory viral infection and of the dengue virus gene.

  17. Testing gold nanostructures fabricated by hole-mask colloidal lithography as potential substrates for SERS sensors: sensitivity, signal variability, and the aspect of adsorbate deposition.

    Science.gov (United States)

    Peksa, Vlastimil; Lebrušková, Petra; Šípová, Hana; Štěpánek, Josef; Bok, Jiří; Homola, Jiří; Procházka, Marek

    2016-07-20

    Gold nanoplasmonic substrates with high sensitivity and spectral reproducibility are key components of molecular sensors based on surface-enhanced Raman scattering (SERS). In this work, we used a confocal Raman microscope and several types of gold nanostructures (arrays of nanodiscs, nanocones and nanodisc dimers) prepared by hole-mask colloidal lithography (HCL) to determine the sources of variability in SERS measurements. We demonstrate that significant variations in the SERS signal can originate from the method of deposition of analyte molecules onto a SERS substrate. While the method based on incubation of SERS substrates in a solution containing the analyte yields a SERS signal with low variability, the droplet deposition method produces a SERS signal with rather high variability. Variability of the SERS signal of a single nanoparticle was determined from the statistical analysis of the SERS signal in short-range Raman maps recorded using different sized laser spots produced by means of different objectives. We show that the number of nanoparticles located within the laser spot can be a source of substantial SERS signal variability, especially for high-magnification objectives. We demonstrate that SERS substrates prepared by HCL exhibit high SERS enhancement and excellent homogeneity (about 20% relative standard deviation from short-range maps). The nanocone arrays are shown to provide the highest SERS enhancement, the lowest relative level of fluorescence background, and also slightly better homogeneity when compared with arrays of nanodisc dimers or single nanodiscs.

  18. Development of SERS substrate using phage-based magnetic template for triplex assay in sepsis diagnosis.

    Science.gov (United States)

    Nguyen, Anh H; Shin, Yesol; Sim, Sang Jun

    2016-11-15

    Development of a new substrate for surface-enhanced Raman scattering (SERS) is one area of interest for the improvement of SERS performance. Herein, we introduce a new method for developing new mesoporous SERS substrates using M13 phages that display cysteine-rich peptides on the pVIII major units, which is an alternative for thiol donor using chemical modifications. Together with the SERS substrate development, and the use of the SERS technique for sepsis diagnostics is a new approach in clinical settings. The substrates were characterized and magnetized with magnetic immuno colloids made of gold-coated magnetic nanoparticles and specific antibodies. Conventionally, the SERS-tags are prepared by using gold nanoparticles and are modified with Raman dyes to immobilize specific antibodies to capture the biomarkers in the serum samples. However, in this method the SERS-tags are bound to the mesoporous substrate via antibody/antigen interactions to form clusters or layer-by-layer assemblies of SERS-tags for Raman signal enhancement. The SERS spectra showed distinct peaks for tags corresponding to three typical sepsis-specific biomarkers for diagnostics with the limit of detection values of 27 pM, 103 pM, and 78 pM for C-reactive protein (CRP), procalcitonin (PCT), and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), respectively. With such an approach, SERS can be used for clinical purposes and can be improved by phage display modification rather than chemical alternatives. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. 29 CFR 1401.37 - Annual report.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Annual report. 1401.37 Section 1401.37 Labor Regulations... Disclosure of Information § 1401.37 Annual report. The Office of the Director shall annually, within 60 days following the close of each calendar year, prepare a report covering each of the categories or records to...

  20. 34 CFR 601.20 - Annual report.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 3 2010-07-01 2010-07-01 false Annual report. 601.20 Section 601.20 Education... Covered Institutions and Institution-Affiliated Organizations § 601.20 Annual report. Each covered... preferred lender arrangement, must— (a) Prepare and submit to the Secretary an annual report, by a...

  1. Risø annual report 2001

    DEFF Research Database (Denmark)

    2002-01-01

    In this annual report, we present a small selection of Risø’s achievements in 2001. A more detailed review of Risø’s projects can be found in the Risø Annual Accounts for 2001 as well as in the annual progress reports prepared by the individual researchdepartments....

  2. 7 CFR 915.32 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Annual report. 915.32 Section 915.32 Agriculture... Regulating Handling Administrative Body § 915.32 Annual report. The committee shall, as soon as practicable after the close of each fiscal year, prepare and mail an annual report to the Secretary, and to...

  3. 15 CFR 9.11 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Annual report. 9.11 Section 9.11... FOR HOUSEHOLD APPLIANCES AND EQUIPMENT TO EFFECT ENERGY CONSERVATION § 9.11 Annual report. The Secretary will prepare an annual report of activities under the program, including an evaluation of...

  4. 7 CFR 922.34 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Annual report. 922.34 Section 922.34 Agriculture... IN WASHINGTON Order Regulating Handling Administrative Body § 922.34 Annual report. The committee shall, prior to the last day of each fiscal period, prepare and mail an annual report to the...

  5. 36 CFR 901.5 - Annual report.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Annual report. 901.5 Section... CORPORATION § 901.5 Annual report. The Executive Director shall prepare annually a comprehensive and detailed report of the Corporation's operations, activities, and accomplishments for the review of the Board...

  6. 32 CFR 48.601 - Annual report.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false Annual report. 48.601 Section 48.601 National... SERVICEMAN'S FAMILY PROTECTION PLAN Miscellaneous § 48.601 Annual report. Information and data for the preparation of the annual report of the Board of Actuaries will be compiled by the Office of the Secretary...

  7. 32 CFR 295.8 - Annual report.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Annual report. 295.8 Section 295.8 National... INFORMATION ACT PROGRAM OFFICE OF THE INSPECTOR GENERAL, FREEDOM OF INFORMATION ACT PROGRAM § 295.8 Annual report. The FOIA Annual Report, assigned Report Control System DD-PA (A) 1365, will be prepared by...

  8. 42 CFR 456.712 - Annual report.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Annual report. 456.712 Section 456.712 Public... Management System for Outpatient Drug Claims § 456.712 Annual report. (a) DUR Board report. The State must require the DUR Board to prepare and submit an annual DUR report to the Medicaid agency that...

  9. 7 CFR 916.34 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Annual report. 916.34 Section 916.34 Agriculture... Regulating Handling Administrative Body § 916.34 Annual report. The committee shall, as soon as is practicable after the close of each marketing season, prepare and mail an annual report to the Secretary...

  10. 15 CFR 16.14 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Annual report. 16.14 Section 16.14 Commerce and Foreign Trade Office of the Secretary of Commerce PROCEDURES FOR A VOLUNTARY CONSUMER PRODUCT INFORMATION LABELING PROGRAM § 16.14 Annual report. The Secretary will prepare an annual report of...

  11. 7 CFR 920.34 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Annual report. 920.34 Section 920.34 Agriculture... Administrative Body § 920.34 Annual report. The committee shall, as soon as is practicable after the close of each marketing season, prepare and mail an annual report to the Secretary and make a copy available...

  12. 7 CFR 923.34 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Annual report. 923.34 Section 923.34 Agriculture... COUNTIES IN WASHINGTON Order Regulating Handling Administrative Body § 923.34 Annual report. The committee shall, prior to the last day of each fiscal period, prepare and mail an annual report to the...

  13. 7 CFR 924.34 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Annual report. 924.34 Section 924.34 Agriculture....34 Annual report. The committee shall, prior to the last day of each fiscal period, prepare and mail an annual report to the Secretary and make a copy available to each handler and grower who requests...

  14. 7 CFR 925.32 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Annual report. 925.32 Section 925.32 Agriculture... SOUTHEASTERN CALIFORNIA Administrative Body § 925.32 Annual report. The committee should, as soon as practicable, after the close of each fiscal period, prepare and mail an annual report to the Secretary...

  15. 7 CFR 947.35 - Annual report.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Annual report. 947.35 Section 947.35 Agriculture... Administrative Committee § 947.35 Annual report. The committee shall prepare and submit to the Secretary, within 2 months following the last day of each fiscal period, an annual report covering such fiscal...

  16. SERS based immuno-microwell arrays for multiplexed detection of foodborne pathogenic bacteria

    Science.gov (United States)

    Sun, Jian; Hankus, Mikella E.; Cullum, Brian M.

    2009-05-01

    A novel surface enhanced Raman scattering (SERS)-based immuno-microwell array has been developed for multiplexed detection of foodborne pathogenic bacteria. The immuno-microwell array was prepared by immobilizing the optical addressable immunomagnetic beads (IMB) into the microwell array on one end of a fiber optic bundle. The IMBs, magnetic beads coated with specific antibody to specific bacteria, were used for immunomagnetic separation (IMS) of corresponding bacteria. The magnetic separation by the homemade magnetic separation system was evaluated in terms of the influences of several important parameters including the beads concentration, the sample volume and the separation time. IMS separation efficiency of the model bacteria E.coli O157:H7 was 63% in 3 minutes. The microwell array was fabricated on hydrofluoric acid etched end of a fiber optic bundle containing 30,000 fiber elements. After being coated with silver, the microwell array was used as a uniform SERS substrate with the relative standard deviation of the SERS enhancement across the microwell array < 2% and the enhancement factor as high as 2.18 x 107. The antibody modified microwell array was prepared for bacteria immobilization into the microwell array, which was characterized by a sandwich immunoassay. To demonstrate the potential of multiplexed SERS detection with the immuno-microwell array, the SERS spectra of different Raman dye labeled magnetic beads as well as mixtures were measured on the mircrowell array. In bead mixture, different beads were identified by the characteristic SERS bands of the corresponding Raman label.

  17. Detection and analysis of SERS effect of nano gold by self-assembly chemical plating composite method

    Science.gov (United States)

    Wang, Chunyan; Xu, Yi; Zhao, Huazhou; Gang, Chen; Lai, Chunhong; Liao, Xin; Wang, Rong

    2015-10-01

    Surface-enhanced Raman spectroscopy (SERS) as a powerful analytical tool has gained extensive attention. Despite of many efforts have been devoted to design SERS substrates, it still remains a grand challenge for creating a general substrate with high SERS active by a simple, controllable and low-cost way. Herein, we attempt to address this issue by fabricating SERS-active nano gold film based on self-assembly chemical plating composite method. The influence of gold colloid particle size on gold seed distribution density and the influence of the particle size of nano gold films on the SERS activity were investigated in detail. 10-5 M Rhodamine 6G (R6G) was taken as Raman probe molecules and homemade Raman detection microsystem was used as detector. According to the results of SERS detection, the SERS substrate enhancement factors (SSEF) were calculated, and the nano gold film with average particle size of about 55 nm showed the maximum SSEF of 0.93 × 105, which indicated such nano gold films showed excellent SERS activity. Moreover, these nano gold films are all low-cost and easy to prepared. This study provided theoretical and experimental basis for the design and preparation of general SERS substrate.

  18. Ver y ser visto en Calicalabozo

    Directory of Open Access Journals (Sweden)

    Santiago Guevara

    2014-01-01

    Full Text Available El presente artículo pretende dar cuenta de la manera en que el territorio urbano es presentado en el libro de cuentos Calicalabozo de Andrés Caicedo. Se analiza la dicotomía; ver y ser visto. El objetivo es brindar una aproximación al imaginario de la ciudad que Caicedo propone en su libro.

  19. Synthesis of Dendritic Silver Nanoparticles and Their Applications as SERS Substrates

    Directory of Open Access Journals (Sweden)

    Jinshan Yu

    2013-01-01

    Full Text Available The silver nanoparticles are synthesized by electrodeposition in ultradilute Ag+ concentration electrolyte under high overpotential. The as prepared Ag nanoparticles, with the sizes ranging from 20 to 30 nm, are arrayed orderly and formed dendritic morphology. The formation of this special dendritic nanoparticle structure can be contributed to the relatively high growth rate and the preferential growth directions along 111 due to the high overpotential, as well as the relative small number of Ag+ ions arriving at the Ag crystal surface per unit time due to the ultradilute Ag+ concentration. Surface enhanced Raman scattering (SERS experiments reveal that the as-prepared dendritic Ag nanoparticles possess high SERS properties and can be used as a candidate substrate for practical SERS applications to detect the Rhodamine 6G molecules.

  20. Controllable SERS performance for the flexible paper-like films of reduced graphene oxide

    Science.gov (United States)

    Yan, Taotao; Zhang, Lili; Jiang, Tongtong; Bai, Zhiman; Yu, Xinxin; Dai, Peng; Wu, Mingzai

    2017-10-01

    paper-like films of reduced graphene oxide with controllable shapes and thickness were prepared via funnel filtration and thermal reduction of graphene oxide (GO), which could work as flexible surface-enhanced Raman scattering (SERS) substrates to detect Rhodamine 6G (R6G) molecules. The SERS signal intensity of R6G on annealed films was found to be related with the annealling temperature. The most intense signal was observed for the film obtained at 800 °C. While for the sample annealed at 1000 °C, no SERS signal could be detected. The underlying mechanism for the difference was discussed. The reported preparation process of flexible rGO paper is simple and cost-effective, which shows great potential in rapid biochemical analysis of agricultural products with irregular surfaces.

  1. Annual Site Environmental Report Prepared for the Department of Energy,under contract number DE-AC03-76SF00515 January - December 2001

    Energy Technology Data Exchange (ETDEWEB)

    Russak, Hillary M.

    2003-05-06

    This report provides information about environmental programs during 2001 at the Stanford Linear Accelerator Center (SLAC). Also included are seasonal activities that cross calendar-year divisions. Production of the annual site environmental report (ASER) is a requirement established by the US Department of Energy (DOE) for all management and operating (M&O) contractors throughout the DOE complex. SLAC is federally-funded, research development center with Stanford University as the M and O contractor. The most noteworthy information in this report is summarized in this section. This summary demonstrates the effective application of SLAC environmental management in meeting the site's Integrated Safety Management System (ISMS) goals. For normal daily activities, all SLAC managers and supervisors are responsible for ensuring that proper procedures are followed to meet the ISMS goals: Worker safety and health are protected; The environment is protected; and Compliance is assured. Throughout 2001, SLAC focused on these activities through the SLAC management systems. These systems were also the ways SLAC approached implementing the ''Greening of the Government'' initiatives (such as Executive Order 13148). The management systems at SLAC are effective, supporting compliance with all relevant statutory and regulatory requirements. SLAC did not receive any notices of violation during 2001. In addition, many improvements were continued during 2001, including improvements in the storm drain system, improved ground water monitoring capabilities, and enhancements to the system to manage the generation of mixed wastes. Environmental program-specific summary details are shown below.

  2. Enseñar a ser

    OpenAIRE

    García Barreno, Pedro

    2004-01-01

    El «Informe Delors», cuyo texto fue presentado hace ahora diez años, señala que cualquier proyecto educativo para el siglo XXI debe contemplar dos acciones. «Enseñar a conocer, a hacer, a vivir juntos, a ser», y garantizar el acceso de todos a ello. El siglo que concluyó apenas media docena de años, ha estado cargado de inmensas paradojas: entre grandes males, extraordinarios logros y no pocas esperanzas inalcanzadas. Marcado por dos guerras mundiales, po...

  3. ConoSer en la Universidad

    Directory of Open Access Journals (Sweden)

    Nina Cabra

    2007-10-01

    Full Text Available La Universidad contemporánea debe asumir la pregunta por las formas de comunicación-educación que dinamiza en su seno. La necesidad de superar los modelos transmisionistas, implica la configuración de nuevos viajes en la formación, en la investigación y en la gestión de procesos sociales. Este artículo presenta una reflexión sobre las posibilidades del cuerpo y la sensibilidad como fuentes de conocimiento, exploración del ser y catalizadores del pensar en el hacer de la Universidad.

  4. ConoSer en la Universidad

    OpenAIRE

    Nina Cabra

    2007-01-01

    La Universidad contemporánea debe asumir la pregunta por las formas de comunicación-educación que dinamiza en su seno. La necesidad de superar los modelos transmisionistas, implica la configuración de nuevos viajes en la formación, en la investigación y en la gestión de procesos sociales. Este artículo presenta una reflexión sobre las posibilidades del cuerpo y la sensibilidad como fuentes de conocimiento, exploración del ser y catalizadores del pensar en el hacer de la Universidad.

  5. 关于年度财务决算管理要求及报表编制问题理解%Understanding of Management Requirements on Annual Financial Accounts and Statement Preparation Problem

    Institute of Scientific and Technical Information of China (English)

    张君燕

    2014-01-01

    作为集团公司每年要求各部及子公司对本年度各项任务指标完成情况进行分析说明,出具决算报表。通过对财务预算到财务决算的编制及执行,就年度财务决算管理的标准、要求,财务报表编制的涵盖内容及相关主要指标的理解与大家进行分享。%As a group company, it asks departments and subsidiaries to analyze the completion situation of the tasks in the year and issue final statements. Through the preparation and execution of financial budget and the financial accounts, the author shows her understanding on the standard and requirements of annual financial accounts management, the covering content and key indicators of the financial statement preparation to share with others.

  6. Determination of histamine in fish by Surface Enhanced Raman Spectroscopy using silver colloid SERS substrates.

    Science.gov (United States)

    Janči, Tibor; Valinger, Davor; Gajdoš Kljusurić, Jasenka; Mikac, Lara; Vidaček, Sanja; Ivanda, Mile

    2017-06-01

    This study was focused on development of a rapid and sensitive method for histamine determination in fish based on Surface Enhanced Raman Spectroscopy (SERS) using simple and widely available silver colloid SERS substrate. Extraction of histamine with 0.4M perchloric acid and purification with 1-butanol significantly shortened sample preparation (30min) and provided clear SERS spectra with characteristic Raman bands of histamine. Principal component analysis effectively distinguished SERS spectra of fish samples with different histamine content. Partial least square (PLS) regression models confirmed reliability of detection and spectral analysis of histamine with SERS. In histamine concentration range 0-200mgkg(-1), significant in legislative and fish quality control aspects, PLS regression model based on spectral range 1139.9-1643.7cm(-1) showed linear trend with R(2)pred=0.962, RPD=7.250. Presented protocol for histamine extraction and purification followed by SERS analysis coupled with chemometric approach, enabled development of rapid and inexpensive method for histamine determination in fish.

  7. internacionales ser considerados actos no justiciables?

    Directory of Open Access Journals (Sweden)

    Eleonora Mesquita Ceia

    2007-01-01

    Full Text Available La premisa del presente artículo es que el control de constitucionalidad de los tratados internacionales puede afectar a la política exterior del Ejecutivo. A partir de esta hipótesis, el autor analiza si dichos instrumentos deben ser considerados actos no justiciables, o si en el caso de que el argumento a favor de la justiciabilidad prevalezca, si ellos deben por lo menos merecer un tratamiento especial. La cuestión será examinada desde cuatro perspectivas jurídicas diferentes: la estadounidense, la francesa, la alemana y la brasileña. Basado en los resultados de este análisis legal comparativo, el artículo concluye a favor de la justiciabilidad de los tratados internacionales y, por otra parte, propone una alternativa que proporcione el equilibrio entre el principio de la separación de poderes y el carácter complejo de las relaciones internacionales.

  8. Hollow plasmonic antennas for broadband SERS spectroscopy.

    Science.gov (United States)

    Messina, Gabriele C; Malerba, Mario; Zilio, Pierfrancesco; Miele, Ermanno; Dipalo, Michele; Ferrara, Lorenzo; De Angelis, Francesco

    2015-01-01

    The chemical environment of cells is an extremely complex and multifaceted system that includes many types of proteins, lipids, nucleic acids and various other components. With the final aim of studying these components in detail, we have developed multiband plasmonic antennas, which are suitable for highly sensitive surface enhanced Raman spectroscopy (SERS) and are activated by a wide range of excitation wavelengths. The three-dimensional hollow nanoantennas were produced on an optical resist by a secondary electron lithography approach, generated by fast ion-beam milling on the polymer and then covered with silver in order to obtain plasmonic functionalities. The optical properties of these structures have been studied through finite element analysis simulations that demonstrated the presence of broadband absorption and multiband enhancement due to the unusual geometry of the antennas. The enhancement was confirmed by SERS measurements, which showed a large enhancement of the vibrational features both in the case of resonant excitation and out-of-resonance excitation. Such characteristics indicate that these structures are potential candidates for plasmonic enhancers in multifunctional opto-electronic biosensors.

  9. Metal Nanoparticles Deposited on Porous Silicon Templates as Novel Substrates for SERS

    Directory of Open Access Journals (Sweden)

    Lara Mikac

    2015-12-01

    Full Text Available In this paper, results on preparation of stable and uniform SERS solid substrates using macroporous silicon (pSi with deposited silver and gold are presented. Macroporous silicon is produced by anodisation of p-type silicon in hydrofluoric acid. The as prepared pSi is then used as a template for Ag and Au depositions. The noble metals were deposited in three different ways: by immersion in silver nitrate solution, by drop-casting silver colloidal solution and by pulsed laser ablation (PLA. Substrates obtained by different deposition processes were evaluated for SERS efficiency using methylene blue (MB and rhodamine 6G (R6G at 514.5, 633 and 785 nm. Using 514.5 nm excitation and R6G the limits of detection (LOD for macroporous Si samples with noble metal nanostructures obtained by immersion of pSi sample in silver nitrate solution and by applying silver colloidal solution to pSi template were 10–9 M and 10–8 M respectively. Using 633 nm laser and MB the most noticeable SERS activity gave pSi samples ablated with 30000 and 45000 laser pulses where the LODs of 10–10 M were obtained. The detection limit of 10–10 M was also reached for 4 mA cm–2-15 min pSi sample, silver ablated with 30000 pulses. Macroporous silicon proved to be a good base for the preparation of SERS substrates.

  10. Nanostructured Silver Substrates With Stable and Universal SERS Properties: Application to Organic Molecules and Semiconductor Nanoparticles

    Directory of Open Access Journals (Sweden)

    Waurisch C

    2009-01-01

    Full Text Available Abstract Nanostructured silver films have been prepared by thermal deposition on silicon, and their properties as SERS substrates investigated. The optimal conditions of the post-growth annealing of the substrates were established. Atomic force microscopy study revealed that the silver films with relatively dense and homogeneous arrays of 60–80-nm high pyramidal nanoislands are the most efficient for SERS of both organic dye and inorganic nanoparticles analytes. The noticeable enhancement of the Raman signal from colloidal nanoparticles with the help of silver island films is reported for the first time.

  11. Tuning plasmonic and chemical enhancement for SERS detection on graphene-based Au hybrids

    Science.gov (United States)

    Liang, Xiu; Liang, Benliang; Pan, Zhenghui; Lang, Xiufeng; Zhang, Yuegang; Wang, Guangsheng; Yin, Penggang; Guo, Lin

    2015-11-01

    Various graphene-based Au nanocomposites have been developed as surface-enhanced Raman scattering (SERS) substrates recently. However, efficient use of SERS has been impeded by the difficulty of tuning SERS enhancement effects induced from chemical and plasmonic enhancement by different preparation methods of graphene. Herein, we developed graphene-based Au hybrids through physical sputtering gold NPs on monolayer graphene prepared by chemical vapor deposition (CVD) as a CVD-G/Au hybrid, as well as graphene oxide-gold (GO/Au) and reduced-graphene oxide (rGO/Au) hybrids prepared using the chemical in situ crystallization growth method. Plasmonic and chemical enhancements were tuned effectively by simple methods in these as-prepared graphene-based Au systems. SERS performances of CVD-G/Au, rGO/Au and GO/Au showed a gradually monotonic increasing tendency of enhancement factors (EFs) for adsorbed Rhodamine 6G (R6G) molecules, which show clear dependence on chemical bonds between graphene and Au, indicating that the chemical enhancement can be steadily controlled by chemical groups in a graphene-based Au hybrid system. Most notably, we demonstrate that the optimized GO/Au was able to detect biomolecules of adenine, which displayed high sensitivity with a detection limit of 10-7 M as well as good reproducibility and uniformity.Various graphene-based Au nanocomposites have been developed as surface-enhanced Raman scattering (SERS) substrates recently. However, efficient use of SERS has been impeded by the difficulty of tuning SERS enhancement effects induced from chemical and plasmonic enhancement by different preparation methods of graphene. Herein, we developed graphene-based Au hybrids through physical sputtering gold NPs on monolayer graphene prepared by chemical vapor deposition (CVD) as a CVD-G/Au hybrid, as well as graphene oxide-gold (GO/Au) and reduced-graphene oxide (rGO/Au) hybrids prepared using the chemical in situ crystallization growth method. Plasmonic

  12. Are there priority competencies for preparation of accountants?: A study about Brazilian accountants ¿Existen competencias que deben ser prioridad en el desarrollo del contador?: Un estudio sobre los contadores Existem competências a serem priorizadas no desenvolvimento do contador?: Um estudo sobre os contadores brasileiros

    Directory of Open Access Journals (Sweden)

    Ricardo Lopes Cardoso

    2010-09-01

    Full Text Available This study sought to determine the need for priority of competencies in preparation of accountants as well as variations between men and women. This question is motivated by Hardern (1995, Morgan (1997, Moramed and Lashine (2003and IFAC (2003 with a theoretical foundation of competencies related to McClelland (1973, 1998 and Boyatzis (1982. Professional references are Hardern (1995, AICPA (1999 and IFAC (2003. It was based on 18 competencies that were collected from 24 articles for questions submitted to 159 accountants and the data collection instrument showed a 0.8774 Cronbach alpha. Replies were submitted to the Kruskal-Wallis test which showed a significance level of El objetivo principal de este estudio es verificar si existen competencias que deben ser prioridad en el desarrollo del contador, como también analizar si esa prioridad se altera entre hombres y mujeres. Ese cuestionamiento tiene como motivación las observaciones de Hardern (1995, Morgan (1997 y IFAC (2003. El fundamento teórico sobre competencias está relacionado al estudio de McClelland (1973,1998, Boyatzis (1982 y Spencer y Spencer (1993. Los estudios relacionados a las competencias del contador no están basados en fundamentos de la Psicología y de Recursos Humanos, y ni siquiera en el análisis de estructuras genéricas de competencias o en la determinación de prioridades en el desarrollo de competencias de los contadores, esta última es el objetivo de este trabajo. La investigación está basada en el estudio de 18 competencias del contador y fue submetida a 159 contadores. El instrumento de recolección de los datos presentó un Alfa de Cronbach de 0,8774. A partir de las respuestas se realizó el test de Kruskal-Wallis, que demostró un nivel de significancia O objetivo principal deste estudo é verificar se existem competências a serem priorizadas no desenvolvimento do contador, bem como analisar se essa prioridade se altera entre homens e mulheres. Esse

  13. Enseñar a ser

    Directory of Open Access Journals (Sweden)

    García Barreno, Pedro

    2004-09-01

    Full Text Available El «Informe Delors», cuyo texto fue presentado hace ahora diez años, señala que cualquier proyecto educativo para el siglo XXI debe contemplar dos acciones. «Enseñar a conocer, a hacer, a vivir juntos, a ser», y garantizar el acceso de todos a ello. El siglo que concluyó apenas media docena de años, ha estado cargado de inmensas paradojas: entre grandes males, extraordinarios logros y no pocas esperanzas inalcanzadas. Marcado por dos guerras mundiales, por genocidios, por la acelerada degradación de la biosfera o por la miseria de una masa de marginados frente al creciente bienestar de los privilegiados.…

  14. euro a ser una moneda internacional

    Directory of Open Access Journals (Sweden)

    María Hortensia Lacayo Ojeda

    2001-01-01

    Full Text Available En este trabajo se estudia cuáles son los principales factores económicos y financieros que llevan al euro, la nueva moneda común europea, a ser una moneda internacional junto al dólar y al yen. Entre dichos factores se encuentran, en primer lugar, el respaldo económico de los doce países de la Unión Económica y Monetaria medido a través de su importancia demográfica, su producto interno bruto (PIB y su grado de apertura comercial; en segundo lugar, la dimensión financiera de la zona euro en lo referente a las inversiones realizadas en la moneda europea, su utilización como moneda de reserva y como moneda de intercambio; en tercer término, la confianza que inspire el euro a los actores económicos

  15. SERS detection of biomolecules using lithographed nanoparticles towards a reproducible SERS biosensor

    Energy Technology Data Exchange (ETDEWEB)

    David, Catalina; Guillot, Nicolas; Chapelle, Marc Lamy de la [Laboratoire CSPBAT (FRE 3043), UFR SMBH, Universite Paris XIII, 74 rue Marcel Cachin, F-93017 Bobigny (France); Shen, Hong; Toury, Timothee, E-mail: marc.lamydelachapelle@univ-paris13.fr [ICD-LNIO-UMR, CNRS 6279, Universite de technologie de Troyes, 12 rue Marie Curie, F-10010 Troyes (France)

    2010-11-26

    In this paper we highlight the accurate spectral detection of bovine serum albumin and ribonuclease-A using a surface-enhanced Raman scattering (SERS) substrate based on gold nanocylinders obtained by electron-beam lithography (EBL). The nanocylinders have diameters from 100 to 180 nm with a gap of 200 nm. We demonstrate that optimizing the size and the shape of the lithographed gold nanocylinders, we can obtain SERS spectra of proteins at low concentration. This SERS study enabled us to estimate high enhancement factors (10{sup 5} for BSA and 10{sup 7} for RNase-A) of important bands in the protein Raman spectrum measured for 1 mM concentration. We demonstrate that, to reach the highest enhancement, it is necessary to optimize the SERS signal and that the main parameter of optimization is the LSPR position. The LSPR have to be suitably located between the laser excitation wavelength, which is 632.8 nm, and the position of the considered Raman band. Our study underlines the efficiency of gold nanocylinder arrays in the spectral detection of proteins.

  16. Feasibility of colloidal silver SERS for rapid bacterial screening

    Science.gov (United States)

    Citrate-reduced silver colloids have been used extensively for surface-enhanced Raman scattering (SERS) study and are commonly characterized by UV-visible spectroscopy. In this work, relative standard deviation (RSD) of SERS spectra from silver colloidal suspensions and ratios of SERS peaks from sma...

  17. A novel paper rag as 'D-SERS' substrate for detection of pesticide residues at various peels.

    Science.gov (United States)

    Zhu, Yiqun; Li, Minqiang; Yu, Daoyang; Yang, Liangbao

    2014-10-01

    Many important considerations in the design of practical Surface enhanced Raman spectroscopy (SERS) substrates are necessary, such as the low cost, simple preparation, mass production and high efficiency of sample collection, which the conventional rigid substrates are lack of. In this work, practical SERS substrates based on deposition of silver nanoparticles (Ag NPs) on commercially available low-cost filter paper were prepared by simple silver mirror reaction in a large scale, and utilized for rapid, portable and accurate identification and detection of pesticide residues at various peels. Compared with conventional substrates, this novel SERS substrate dramatically enhanced the sample collection efficiency by simply swabbing paper-based device across different surfaces without destroying the sample, meanwhile avoiding the substrate signal of real-world samples. Considering their low cost, portability, simplicity and high sample collection efficiency, Ag NP-decorated filter paper, as practical SERS substrate, are used in solving critical problems for detection of pesticide residues at various peels. SERS experiments were carried out on Ag NP-decorated filter paper combined with 'dynamic SERS' (D-SERS) due to its high detection sensitivity. The excellent detection performance of the Ag NP-based filter paper was demonstrated by detection thiram and paraoxon residues at various peels. Besides, the stability and reproducibility of the practical substrates were also involved.

  18. IR, Raman and SERS spectra of 2-(methoxycarbonylmethylsulfanyl)-3,5-dinitrobenzene carboxylic acid

    Energy Technology Data Exchange (ETDEWEB)

    Raj, Asha [Government Polytechnic Coll., Attingal, Kerala (India). Dept. of Physics; Raju, K. [University College, Trivandrum, Kerala (India). Dept. of Physics; Varghese, Hema Tresa [Fatima Mata National Coll., Kollam, Kerala (India). Dept. of Physics; Granadeiro, Carlos M.; Nogueira, Helena I. S. [University of Aveiro (Portugal). Centro de Investigacao em Materiais Ceramicos e Compositos (CICECO). Dept. of Chemistry; Panicker, C. Yohannan [TKM College of Arts and Science, Kollam, Kerala (India). Dept. of Physics]. E-mail: cyphyp@rediffmail.com

    2009-07-01

    2-(Methoxycarbonylmethylsulfanyl)-3,5-dinitrobenzenecarboxylic acid was prepared by nucleophilic substitution. FT-IR and FT-Raman spectra of 2-(methoxycarbonylmethylsulfanyl)-3,5-dinitrobenzenecarboxylic acid were recorded and analyzed. Surface enhanced Raman scattering (SERS) spectrum was recorded on a silver colloid. The vibrational wavenumbers were computed by density functional theoretical (DFT) computations at the B3LYP/6-31G{sup *} level and they were found to be in good agreement with the experimental values. Significant metal-molecule interaction has been substantiated by the appearance of intense Ag-O mode in the SERS spectrum and this is indicative of the nearness of nitro and carbonyl group to the silver surface. SERS studies suggest a tilted orientation of the molecule at the metal surface. (author)

  19. Heidegger y la pregunta por el sentido del ser

    OpenAIRE

    2010-01-01

    La contribución más duradera de Heidegger a la filosofía de nuestra época consiste en la defensa del cometido «ontológico» de la filosofía. En su amplio itinerario planteó la pregunta por el ser de tres maneras: como pregunta por el sentido del ser, la verdad del ser y el lugar del ser. En este artículo examinaremos críticamente su primera formulación: la que desembocó en una de las obras más influyentes del siglo XX, "Ser y tiempo".

  20. Analysis on superhydrophobic silver decorated copper Oxide nanostructured thin films for SERS studies.

    Science.gov (United States)

    Jayram, Naidu Dhanpal; Aishwarya, D; Sonia, S; Mangalaraj, D; Kumar, P Suresh; Rao, G Mohan

    2016-09-01

    The present work demonstrates the superhydrophobic and Surface Enhanced Raman Spectroscopy (SERS) active substrate performance of silver coated copper oxide (Ag@CuO) nanostructured thin films prepared by the SILAR process. Super hydrophobic substrates that combine super hydrophobic condensation effect and high enhancement ability of Ag@CuO nanoflowers are investigated for SERS studies. The possible growth mechanism for the formation of nanoflower arrays from nanospindles has been discussed. Morphology and crystallinity of the Ag@CuO thin films are confirmed using FESEM and XRD. The results obtained in the present study indicate that the as-deposited hydrophobic nanospindles structure converts to super hydrophobic nanoflower arrays on annealing at 200°C. The Ag@CuO super hydrophobic nanoflowers thin film based SERS substrates show highly enhanced Raman spectra with an EF value of 2.0×10(7) for (Rhodamine 6G) R6G, allowing a detection limit from a 10(-10)molL(-1) solution. The present study may provide a new perception in fabricating efficient super hydrophobic substrates for SERS, suggesting that the fabricated substrates are promising candidates for trace analysis of R6G dye and are expected to be widely used as highly sensitive SERS active substrates for various toxic dyes in the future.

  1. Experimental and density functional theory study of Raman and SERS spectra of 5-amino-2-mercaptobenzimidazole.

    Science.gov (United States)

    Chen, Yufeng; Yang, Jin; Li, Zonglong; Li, Ran; Ruan, Weidong; Zhuang, Zhiping; Zhao, Bing

    2016-01-15

    Raman spectroscopy, surface-enhanced Raman spectroscopy (SERS) and density functional theory (DFT) simulations were employed to study 5-amino-2-mercaptobenzimidazole (5-A-2MBI) molecules. Ag colloids were used as SERS substrates which were prepared by using hydroxylamine hydrochloride as reducing agent. Raman vibration modes and SERS characteristic peaks of 5-A-2MBI were assigned with the aid of DFT calculations. The molecular electrostatic potential (MEP) of 5-A-2MBI was used to discuss the possible adsorption behavior of 5-A-2MBI on Ag colloids. The spectral analysis showed that 5-A-2MBI molecules were slightly titled via the sulfur atoms adhering to the surfaces of Ag substrates. The obtained SERS spectral intensity decreased when lowering the 5-A-2MBI concentrations. A final detection limit on the concentration of 5×10(-7) mol · L(-1) was gained. SERS proved to be a simple, fast and reliable method for the detection and characterization of 5-A-2MBI molecules.

  2. Application of silver nanodendrites deposited on silicon in SERS technique for the trace analysis of paraquat

    Science.gov (United States)

    Cao Dao, Tran; Quynh Ngan Luong, Truc; Cao, Tuan Anh; Kieu, Ngoc Minh; Le, Van Vu

    2016-03-01

    In order to detect trace concentrations of organic or biological molecules by surface-enhanced Raman scattering (SERS) technique, the SERS-active substrates with high enhancement factor are required. The silver nanodendrites (AgNDs) are a growing class of such SERS-active substrates. This report presents the preliminary results of the trace detection of paraquat (PQ), a commonly used herbicide, with the use of SERS-active substrates, which have been made from AgNDs deposited on silicon. The AgNDs were produced either by electroless deposition, or by electrodeposition onto a silicon wafer, using aqueous solution of HF and AgNO3. It was observed that the silver dendrites are formed only when AgNO3 concentration is high enough. Next, it was found that with the additional assistance of an electric potential in the electrodeposition, the dendrites have grown up with the more perfect ramification. The AgNDs with more perfect branching gave the Raman spectrum of PQ with higher enhancement factor. More specifically, while the SERS-active substrates prepared from electrodeposited AgNDs were able to detect PQ with concentration as low as 0.01 ppm, the ones made from electroless deposited AgNDs could only detect PQ at concentration of hundreds times higher.

  3. Water security: continuous monitoring of water distribution systems for chemical agents by SERS

    Science.gov (United States)

    Inscore, Frank; Shende, Chetan; Sengupta, Atanu; Farquharson, Stuart

    2007-04-01

    Ensuring safe water supplies requires continuous monitoring for potential poisons and portable analyzers to map distribution in the event of an attack. In the case of chemical warfare agents (CWAs) analyzers are needed that have sufficient sensitivity (part-per-billion), selectivity (differentiate the CWA from its hydrolysis products), and speed (less than 10 minutes) to be of value. We have been investigating the ability of surface-enhanced Raman spectroscopy (SERS) to meet these requirements by detecting CWAs and their hydrolysis products in water. The expected success of SERS is based on reported detection of single molecules, the one-to-one relationship between a chemical and its Raman spectrum, and the minimal sample preparation requirements. Recently, we have developed a simple sampling device designed to optimize the interaction of the target molecules with the SERS-active material with the goal of increasing sensitivity and decreasing sampling times. This sampling device employs a syringe to draw the water sample containing the analyte into a capillary filled with the SERS-active material. Recently we used such SERS-active capillaries to measure 1 ppb cyanide in water. Here we extend these measurements to nerve agent hydrolysis products using a portable Raman analyzer.

  4. Detection of Adhesion Molecules on Inflamed Macrophages at Early-Stage Using SERS Probe Gold Nanorods

    Institute of Scientific and Technical Information of China (English)

    Dakrong Pissuwan; Yusuke Hattori

    2017-01-01

    In recent years, it has been shown that inflammatory biomarkers can be used as an effective signal for disease diagnoses. The early detection of these signals provides useful information that could prevent the occurrence of severe diseases. Here, we employed surface-enhanced Raman scattering (SERS) probe gold nanorods (GNRs) as a tool for the early detection of inflammatory molecules in inflamed cells. A murine macrophage cell line (Raw264.7) stimulated with lipopolysaccharide (LPS) was used as a model in this study. The prepared SERS probe GNRs containing 4-mercapto-benzoic acid as a Raman reporter to generate SERS signals were used for detection of intracellular adhesion molecule-1 (ICAM-1) in macrophages after treatment with LPS for varying lengths of time. Our results show that SERS probe GNRs could detect significant differences in the expression of ICAM-1 molecules in LPS-treated macrophages compared to those in untreated macrophages after only 1 h of LPS treatment. In contrast, when using fluorescent labeling or enzyme-linked immunosorbent assays (ELISA) to detect ICAM-1, significant differences between inflamed and un-inflamed macrophages were not seen until the cells had been treated with LPS for 5 h. These results indicate that our SERS probe GNRs provide a higher sensitivity for detecting biomarker molecules in inflamed macrophages than the conventional fluorescence and ELISA techniques, and could therefore be useful as a potential diagnostic tool for managing disease risk.

  5. Fabrication of SERS Active Surface on Polyimide Sample by Excimer Laser Irradiation

    Directory of Open Access Journals (Sweden)

    T. Csizmadia

    2014-01-01

    Full Text Available A possible application of excimer laser irradiation for the preparation of surface enhanced Raman spectroscopy (SERS substrate is demonstrated. A polyimide foil of 125 μm thickness was irradiated by 240 pulses of focused ArF excimer laser beam (λ = 193 nm, FWHM = 20 ns. The applied fluence was varied between 40 and 80 mJ/cm2. After laser processing, the sample was coated with 40 nm silver by PLD in order to create a conducting layer required for the SERS application. The SERS activity of the samples was tested by Raman microscopy. The Raman spectra of Rhodamine 6G aqueous solution (c=10−3 mol/dm3 were collected from the patterned and metalized areas. For areas prepared at 40–60 mJ/cm2 laser fluences, the measured Raman intensities have shown a linear dependence on the applied laser fluence, while above 60 mJ/cm2 saturation was observed. The morphology of the SERS active surface areas was investigated by scanning electron microscopy. Finite element modeling was performed in order to simulate the laser-absorption induced heating of the polyimide foil. The simulation resulted in the temporal and spatial distribution of the estimated temperature in the irradiated polyimide sample, which are important for understanding the structure formation process.

  6. The planets around NN Ser: still there

    CERN Document Server

    Marsh, T R; Bours, M C P; Littlefair, S P; Copperwheat, C M; Dhillon, V S; Breedt, E; Caceres, C; Schreiber, M R

    2013-01-01

    We present 25 new eclipse times of the white dwarf binary NN Ser taken with the high-speed camera ULTRACAM on the WHT and NTT, the RISE camera on the Liverpool Telescope, and HAWK-I on the VLT to test the two-planet model proposed to explain variations in its eclipse times measured over the last 25 years. The planetary model survives the test with flying colours, correctly predicting a progressive lag in eclipse times of 36 seconds that has set in since 2010 compared to the previous 8 years of precise times. Allowing both orbits to be eccentric, we find orbital periods of 7.9 +/- 0.5 yr and 15.3 +/- 0.3 yr, and masses of 2.3 +/- 0.5 Mjup and 7.3 +/- 0.3 Mjup. We also find dynamically long-lived orbits consistent with the data, associated with 2:1 and 5:2 period ratios. The data scatter by 0.07 seconds relative to the best-fit model, by some margin the most precise of any of the proposed eclipsing compact object planet hosts. Despite the high precision, degeneracy in the orbit fits prevents a significant measu...

  7. Libertas que serás enfermagem

    Directory of Open Access Journals (Sweden)

    Mary Anne Fontenele Martins

    1998-09-01

    Full Text Available O título deste ensaio nos faz lembrar a Inconfidência Mineira, que teve como líder José Joaquim da Silva Xavier, o Tiradentes, sendo um movimento que ansiava por liberdade, assim como a enfermagem que, ao longo dos anos, vem construindo sua história e caminhando em busca de sua autonomia. A presente pesquisa tem como objetivo investigar os caminhos percorridos pela enfermagem, enquanto ciência na busca de sua autonomia, e refletir sobre a atuação do enfermeiro enquanto profissional criativo e autônomo. Estudo respaldado no materialismo histórico e dialético, realizado no período de maio a agosto de 1997 com enfermeiras do Estado do Ceará, que estão envolvidas com o processo histórico da profissão. Os resultados deram origem a uma categoria central, prática profissional e outras três: autonomia, criatividade e disposição para enfrentar desafios, que favoreceram uma melhor compreensão dos caminhos já trilhados pela enfermagem, além de nos permitir entender que o enfermeiro pode ser autônomo e livre.

  8. MODOS DE SER DA JUVENTUDE OCIDENTAL

    Directory of Open Access Journals (Sweden)

    Prof. Dr. Luiz Antônio Dias

    2015-09-01

    Full Text Available O presente artigo é resultado de investigações ainda em andamento no Grupo de Pesquisa “Culturas juvenis, consumo e mobilidade urbana na contemporaneidade”, iniciadas em 2013 junto ao Programa de Mestrado Interdisciplinar em Ciências Humanas da Universidade de Santo Amaro (UNISA. A pesquisa dedica-se a entender, entre outras reflexões, o percurso dos jovens na sociedade contemporânea e as contribuições que eles trouxeram para repensar o espaço urbano. Nesse estudo buscamos localizar no espaço e no tempo em quais condições históricas o sentimento de juventude apareceu para a sociedade. Em seguida, fazemos um minucioso levantamento da trajetória dessa nova categoria social nos mais diferentes períodos da história contemporânea. Chegamos, assim, ao século XX, momento em que as reuniões juvenis deixam de ser fatos isolados e adquirem conotações de manifestações verdadeiramente sociais. Ainda nessa perspectiva de análise, buscamos compreender as similitudes, aproximações e distanciamentos do movimento punk com seus congêneres do passado.

  9. Paraplejia: pasado y futuro del ser

    Directory of Open Access Journals (Sweden)

    María Elisa Moreno-Fergusson

    Full Text Available El propósito del artículo es presentar una síntesis de la literatura publicada entre 1988 y 2008 sobre los cambios en la corporalidad de las personas con paraplejia secundaria a un trauma raquimedular y su relevancia para el cuidado de enfermería. La revisión incluyó 22 estudios cualitativos, 5 cuantitativos y 20 piezas documentales, publicadas por profesionales de la salud y de las ciencias humanas. Los resultados permiten concluir que las secuelas del trauma raquimedular causan dependencia, alteran la imagen corporal, la interacción social, el sentido de coherencia del ser y la corporalidad. Con el tiempo, estas personas, re-conocen su "nuevo cuerpo" y construyen una nueva corporalidad, proceso que aún no ha sido descrito y que se considera fundamental para el cuidado de enfermería, encaminado a ayudar a las personas con este tipo de lesión para lidiar con las dificultades cotidianas.

  10. Unique Gold Nanoparticle Aggregates as a Highly Active SERS Substrate

    Energy Technology Data Exchange (ETDEWEB)

    Schwartzberg, A M; Grant, C D; Wolcott, A; Talley, C E; Huser, T R; Bogomolni, R; Zhang, J Z

    2004-04-06

    A unique gold nanoparticle aggregate (GNA) system has been shown to be an excellent substrate for surface-enhanced Raman scattering (SERS) applications. Rhodamine 6G (R6G), a common molecule used for testing SERS activity on silver, but generally difficult to detect on gold substrates, has been found to readily bind to the GNA and exhibit strong SERS activity due to the unique surface chemistry afforded by sulfur species on the surface. This GNA system has yielded a large SERS enhancement of 10{sup 7}-10{sup 9} in bulk solution for R6G, on par with or greater than any previously reported gold SERS substrate. SERS activity has also been successfully demonstrated for several biological molecules including adenine, L-cysteine, L-lysine, and L-histidine for the first time on a gold SERS substrate, showing the potential of this GNA as a convenient and powerful SERS substrate for biomolecular detection. In addition, SERS spectrum of R6G on single aggregates has been measured. We have shown that the special surface properties of the GNA, in conjunction with strong near IR absorption, make it useful for SERS analysis of a wide variety of molecules.

  11. A microfluidic dialysis device for complex biological mixture SERS analysis

    KAUST Repository

    Perozziello, Gerardo

    2015-08-01

    In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.

  12. Uranium industry annual 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-07-05

    The Uranium Industry Annual 1994 (UIA 1994) provides current statistical data on the US uranium industry`s activities relating to uranium raw materials and uranium marketing during that survey year. The UIA 1994 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. It contains data for the 10-year period 1985 through 1994 as collected on the Form EIA-858, ``Uranium Industry Annual Survey.`` Data collected on the ``Uranium Industry Annual Survey`` (UIAS) provide a comprehensive statistical characterization of the industry`s activities for the survey year and also include some information about industry`s plans and commitments for the near-term future. Where aggregate data are presented in the UIA 1994, care has been taken to protect the confidentiality of company-specific information while still conveying accurate and complete statistical data. A feature article, ``Comparison of Uranium Mill Tailings Reclamation in the United States and Canada,`` is included in the UIA 1994. Data on uranium raw materials activities including exploration activities and expenditures, EIA-estimated resources and reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities, including purchases of uranium and enrichment services, and uranium inventories, enrichment feed deliveries (actual and projected), and unfilled market requirements are shown in Chapter 2.

  13. A surface-enhanced Raman scattering (SERS-active optical fiber sensor based on a three-dimensional sensing layer

    Directory of Open Access Journals (Sweden)

    Chunyu Liu

    2014-08-01

    Full Text Available To fabricate a new surface-enhanced Raman scattering (SERS-active optical fiber sensor, the design and preparation of SERS-active sensing layer is one of important topics. In this study, we fabricated a highly sensitive three-dimensional (3D SERS-active sensing layer on the optical fiber terminal via in situ polymerizing a porous polymer material on a flat optical fiber terminal through thermal-induced process, following with the photochemical silver nanoparticles growth. The polymerized polymer formed a 3D porous structure with the pore size of 0.29–0.81 μm, which were afterward decorated with abundant silver nanoparticles with the size of about 100 nm, allowing for higher SERS enhancement. This SERS-active optical fiber sensor was applied for the determination of 4-mercaptopyridine, crystal violet and maleic acid The enhancement factor of this SERS sensing layer can be reached as about 108. The optical fiber sensor with high sensitive SERS-active porous polymer is expected for online analysis and environment detection.

  14. SERS spectroscopy of kaempferol and galangin under the interaction of human serum albumin with adsorbed silver nanoparticles

    Science.gov (United States)

    Zhang, Wei; Bai, Xueyuan; Wang, Yingping; Zhao, Bing; Zhao, Daqing; Zhao, Yu

    Raman and surface-enhanced Raman scattering (SERS) spectroscopy were employed to probe the interaction of the flavonol drugs, kaempferol and galangin, with human serum albumin (HSA). SERS spectra of both flavonol derivatives were obtained from a colloidal silver surface in physiological condition, based on the high performance of the enhanced substrate, the most enhanced modes of kaempferol and galangin were those with certain motions perpendicular to the metal surface. The SERS spectra were allowed to predict similar orientation geometry for both of the drugs on the colloidal surface with minor difference. In addition, both flavonols-HSA complexes were prepared in different concentration ratios and the orientated differences between kaempferol and galangin were investigated by SERS.

  15. Uranium industry annual 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-05-01

    The Uranium Industry Annual 1995 (UIA 1995) provides current statistical data on the U.S. uranium industry`s activities relating to uranium raw materials and uranium marketing. The UIA 1995 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. It contains data for the period 1986 through 2005 as collected on the Form EIA-858, ``Uranium Industry Annual Survey``. Data collected on the ``Uranium Industry Annual Survey`` provide a comprehensive statistical characterization of the industry`s plans and commitments for the near-term future. Where aggregate data are presented in the UIA 1995, care has been taken to protect the confidentiality of company-specific information while still conveying accurate and complete statistical data. Data on uranium raw materials activities for 1986 through 1995 including exploration activities and expenditures, EIA-estimated reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities for 1994 through 2005, including purchases of uranium and enrichment services, enrichment feed deliveries, uranium fuel assemblies, filled and unfilled market requirements, uranium imports and exports, and uranium inventories are shown in Chapter 2. The methodology used in the 1995 survey, including data edit and analysis, is described in Appendix A. The methodologies for estimation of resources and reserves are described in Appendix B. A list of respondents to the ``Uranium Industry Annual Survey`` is provided in Appendix C. For the reader`s convenience, metric versions of selected tables from Chapters 1 and 2 are presented in Appendix D along with the standard conversion factors used. A glossary of technical terms is at the end of the report. 14 figs., 56 tabs.

  16. SER MUJER Y SER HOMBRE SEGÚN ALGUNAS MUJERES Y ALGUNOS VARONES SENTENCIADOS POR HOMICIDIO

    Directory of Open Access Journals (Sweden)

    MARITEL YANES

    2016-07-01

    Full Text Available Mediante el uso de los métodos cualitativos, se aplicó un guión temático sobre las opiniones de ser mujer y ser hombre de un grupo de mujeres y varones sentenciados por homicidio recluidos en el Centro de Reinserción del Estado de Tabasco (CRESET. Se buscaba conocer en qué medida sus concepciones de género influenciaron en su comportamiento homicida y observar cuales eran las principales diferencias. Se presentan los resultados del análisis de las respuestas al guion temático de las entrevistas realizadas, donde se observa el criterio de selección y el primer contacto con los entrevistados (as, las condiciones en que se llevaron a cabo las entrevistas, los objetivos y las categorías que se utilizaron para la elaboración del guion. Posteriormente, se describen y comparan las opiniones de las mujeres y de los hombres entrevistados sobre las categorías del guion temático que permiten apreciar múltiples elementos desde la perspectiva de género.

  17. Myofilament Calcium Sensitivity: Mechanistic Insight into TnI Ser-23/24 and Ser-150 Phosphorylation Integration

    Directory of Open Access Journals (Sweden)

    Hussam E Salhi

    2016-12-01

    Full Text Available Troponin I (TnI is a major regulator of cardiac muscle contraction and relaxation. During physiological and pathological stress, TnI is differentially phosphorylated at multiple residues through different signaling pathways to match cardiac function to demand. The combination of these TnI phosphorylations can exhibit an expected or unexpected functional integration, whereby the function of two phosphorylations are different than that predicted from the combined function of each individual phosphorylation alone. We have shown that TnI Ser-23/24 and Ser-150 phosphorylation exhibit functional integration and are simultaneously increased in response to cardiac stress. In the current study, we investigated the functional integration of TnI Ser-23/24 and Ser-150 to alter cardiac contraction. We hypothesized that Ser-23/24 and Ser-150 phosphorylation each utilize distinct molecular mechanisms to alter the TnI binding affinity within the thin filament. Mathematical modeling predicts that Ser-23/24 and Ser-150 phosphorylation affect different TnI affinities within the thin filament to distinctly alter the Ca2+-binding properties of troponin. Protein binding experiments validate this assertion by demonstrating pseudo-phosphorylated Ser-150 decreases the affinity of isolated TnI for actin, whereas Ser-23/24 pseudo-phosphorylation is not different from unphosphorylated. Thus, our data supports that TnI Ser-23/24 affects TnI-TnC binding, while Ser-150 phosphorylation alters TnI-actin binding. By measuring force development in troponin-exchanged skinned myocytes, we demonstrate that the Ca2+ sensitivity of force is directly related to the amount of phosphate present on TnI. Furthermore, we demonstrate that Ser-150 pseudo-phosphorylation blunts Ser-23/24-mediated decreased Ca2+-sensitive force development whether on the same or different TnI molecule. Therefore, TnI phosphorylations can integrate across troponins along the myofilament. These data demonstrate

  18. QUE É O "SER DA FAMÍLIA"?

    Directory of Open Access Journals (Sweden)

    Josefa Aida Delgado

    2005-01-01

    Full Text Available Es un estudio basado en la filosofía fenomenológica heideggeriana, su propósito es desvelar los elementos estructurales de la existencia del "ser de la familia". El camino metodológico fue construido con base en el pensamiento de Heidegger. Los datos de la familia fueron recolectados por medio de las entrevistas y las observaciones. Cada uno de nosotros contribuye para su existencia, y ella posibilita el desarrollo de nuestro "ser-en el-mundo" al vivenciar y compartir experiencias cotidianas de la familia. Allí surge la posibilidad de compartir un modo de ser en el mundo, un modo de cuidado para "ser familia en el mundo". Un mundo que genera esa unidad de relacionamiento que emerge de sentimientos interligados entre los integrantes, respondiendo a las exigencias de cada miembro, por el sentimiento de pertenencia primaria generado en ellos. Esa unidad da la posibilidad de nacer a cada uno de nosotros, asi también, nos da la posibilidad de poder ser ser humano, llegando a ser un referencial de sí misma en cada ser humano.

  19. Fundamentals and applications of SERS-based bioanalytical sensing

    Science.gov (United States)

    Kahraman, Mehmet; Mullen, Emma R.; Korkmaz, Aysun; Wachsmann-Hogiu, Sebastian

    2017-08-01

    Plasmonics is an emerging field that examines the interaction between light and metallic nanostructures at the metal-dielectric interface. Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that uses plasmonics to obtain detailed chemical information of molecules or molecular assemblies adsorbed or attached to nanostructured metallic surfaces. For bioanalytical applications, these surfaces are engineered to optimize for high enhancement factors and molecular specificity. In this review we focus on the fabrication of SERS substrates and their use for bioanalytical applications. We review the fundamental mechanisms of SERS and parameters governing SERS enhancement. We also discuss developments in the field of novel SERS substrates. This includes the use of different materials, sizes, shapes, and architectures to achieve high sensitivity and specificity as well as tunability or flexibility. Different fundamental approaches are discussed, such as label-free and functional assays. In addition, we highlight recent relevant advances for bioanalytical SERS applied to small molecules, proteins, DNA, and biologically relevant nanoparticles. Subsequently, we discuss the importance of data analysis and signal detection schemes to achieve smaller instruments with low cost for SERS-based point-of-care technology developments. Finally, we review the main advantages and challenges of SERS-based biosensing and provide a brief outlook.

  20. A reusable biosensor chip for SERS-fluorescence dual mode immunoassay

    Science.gov (United States)

    Wu, Lei; Wang, Zhuyuan; Fan, Kequan; Zong, Shenfei; Cui, Yiping

    2015-05-01

    Research continues in an effort to develop a versatile platform for clinical diagnosis with easy operation and low cost. In the present study, a biosensor chip has been designed and fabricated for surface enhanced Raman scattering (SERS)- fluorescence dual mode immunoassay. Here, a dual channel microfluidic chip was employed for simultaneous SERS and fluorescence detection. Unlike previously reported microfluidic immunoassays using fluorescence or SERS method independently, the proposed dual mode biosensor combined the advantages of these two optical detection techniques. The fluorescence mode can be used for fast screening of biomolecules while the SERS mode can be employed for accurate and sensitive quantitative analysis. In addition, the chip-based microfluidic platform greatly reduced the reagents cost and complicated operation. The whole detection process from sample preparation to optical detection can be finished in 90 min. Moreover, the reversibly bonded biosensor chip could be reused after cleaning, which further reduced the cost for each assay. All these merits make it a potential powerful tool for practical clinical diagnosis.

  1. Next-generation surface-enhanced Raman scattering (SERS) substrates for hazard detection

    Science.gov (United States)

    Farrell, Mikella E.; Holthoff, Ellen L.; Pellegrino, Paul M.

    2012-06-01

    Sensitive, accurate and reliable methods are needed for the detection and identification of hazardous materials (chemical, biological, and energetic) in the field. Utilizing such a sensing capability incorporated into a portable detection system would have wide spread beneficial impact to the U.S. military and first responder communities. Surface enhanced Raman scattering (SERS) is increasingly becoming a reputable technique for the real-time, dynamic detection and identification of hazard materials. SERS is particularly advantageous as it does not suffer from interferences from water, requires little to no sample preparation, is robust and can be used in numerous environments, is relatively insensitive to the wavelength of excitation employed, and produces a narrow-band spectral signature unique to the molecular vibrations of the analyte. We will report on the characterization and sensing capabilities of these next generation SERS substrates for the detection of energetic materials (ammonium nitrate, TNT, PETN, and RDX). Additionally, new efforts producing highly uniform samples, with known concentrations of energetic materials inkjet printed onto the SERS sensing surface using a precisely calibrated MicroJet system will be shown.

  2. Antibacterial effect of various shapes of silver nanoparticles monitored by SERS.

    Science.gov (United States)

    El-Zahry, Marwa R; Mahmoud, Amer; Refaat, Ibrahim H; Mohamed, Horria A; Bohlmann, Holger; Lendl, Bernhard

    2015-06-01

    A comparative evaluation of antimicrobial effect of synthesized silver nanoparticles (AgNPs) of different shapes using different methods was performed. Spherical, triangular and hexagonal AgNPs with an average size of 40 nm were chemically prepared and characterized by transmission electron microscope (TEM) and UV-visible spectroscopy. The antimicrobial effect of these different AgNPs against the gram negative bacterium Escherichia coli (E. coli) was studied by surface enhanced Raman spectroscopy (SERS), the evaluation of growth curves and inhibition zones. SERS proved to be sensitive to monitor the changes that occurred in the bacterial cells upon interaction with AgNPs, which qualitatively compared well with the data provided by the reference methods. However, as SERS is already sensitive to initial changes in the chemistry of bacteria due to the antibacterial effect of the AgNPs, fast and detailed information is provided by SERS as opposed to the classical reference methods based on the evaluation of growth curves and inhibition zones. The results of this work also demonstrate that hexagonal AgNPs display the highest antibacterial effect when compared to other NPs shapes, with triangular AgNPs exhibiting no antibacterial effect under the adopted conditions.

  3. Photonic Crystal Fibre SERS Sensors Based on Silver Nanoparticle Colloid

    Institute of Scientific and Technical Information of China (English)

    XIE Zhi-Guo; LU Yong-Hua; WANG Pei; LIN Kai-Qun; YAN Jie; MING Hai

    2008-01-01

    @@ A photonic crystal fibre (PCF) surface enhanced Raman scattering (SERS) sensor is developed based on silver nanoparticle colloid.Analyte solution and silver nanoparticles are injected into the air holes of PCF by a simple modified syringe to overcome maes-transport constraints, allowing more silver nanoparticles involved in SERS activity.This sensor offers significant benefit over the conventional SERS sensor with high flexibility, easy manufacture.We demonstrate the detection of 4-mercaptobenzoic acid (4-MBA ) molecules with the injecting way and the common dipping measurement.The injecting way shows obviously better results than the dipping one.Theoretical analysis indicates that this PCF SERS substrate offers enhancement of about 7 orders of magnitude in SERS active area.

  4. SERS decoding of micro gold shells moving in microfluidic systems.

    Science.gov (United States)

    Lee, Saram; Joo, Segyeong; Park, Sejin; Kim, Soyoun; Kim, Hee Chan; Chung, Taek Dong

    2010-05-01

    In this study, in situ surface-enhanced Raman scattering (SERS) decoding was demonstrated in microfluidic chips using novel thin micro gold shells modified with Raman tags. The micro gold shells were fabricated using electroless gold plating on PMMA beads with diameter of 15 microm. These shells were sophisticatedly optimized to produce the maximum SERS intensity, which minimized the exposure time for quick and safe decoding. The shell surfaces produced well-defined SERS spectra even at an extremely short exposure time, 1 ms, for a single micro gold shell combined with Raman tags such as 2-naphthalenethiol and benzenethiol. The consecutive SERS spectra from a variety of combinations of Raman tags were successfully acquired from the micro gold shells moving in 25 microm deep and 75 microm wide channels on a glass microfluidic chip. The proposed functionalized micro gold shells exhibited the potential of an on-chip microfluidic SERS decoding strategy for micro suspension array.

  5. Universal chitosan-assisted synthesis of Ag-including heterostructured nanocrystals for label-free in situ SERS monitoring.

    Science.gov (United States)

    Cai, Kai; Xiao, Xiaoyan; Zhang, Huan; Lu, Zhicheng; Liu, Jiawei; Li, Qin; Liu, Chen; Foda, Mohamed F; Han, Heyou

    2015-12-07

    A universal chitosan-assisted method was developed to synthesize various Ag-including heterostructured nanocrystals, in which chelation probably plays a vital role. The as-prepared Ag/Pd heterostructured nanocrystals show outstanding properties when used as bifunctional nanocomposites in label-free in situ SERS monitoring of Pd-catalyzed reaction.

  6. Adapting and testing a portable Raman spectrometer for SERS analysis of amino acids and small peptides

    Science.gov (United States)

    Brambilla, A.; Philippidis, A.; Nevin, A.; Comelli, D.; Valentini, G.; Anglos, D.

    2013-07-01

    Surface-Enhanced Raman Spectroscopy (SERS), a powerful spectrochemical technique enabling highly sensitive analysis of organic and biological materials, is investigated for applications in the analysis of archaeological materials including in situ screening. In this work, a compact mobile Raman spectrometer is employed for acquiring Surface-Enhanced Raman spectra from natural amino acids (L-Arg, L-Phe, L-Met) and a tripeptide (Glutathione), adsorbed on silver colloids. The detection limits of the portable Raman spectrometer, together with an optimization of sample preparation and experimental parameters, are reported. The collection and interpretation of SER spectra of amino acids and peptides is a starting point for the optimization of the instrumentation and its application in the study of more complex biological molecules in the context of detection and analysis of archaeological materials and residues.

  7. Surface-enhanced vibrational spectroscopy of B vitamins: what is the effect of SERS-active metals used?

    Science.gov (United States)

    Kokaislová, A; Matějka, P

    2012-05-01

    Surface-enhanced Raman scattering (SERS) spectroscopy and surface-enhanced infrared absorption (SEIRA) spectroscopy are analytical tools suitable for the detection of small amounts of various analytes adsorbed on metal surfaces. During recent years, these two spectroscopic methods have become increasingly important in the investigation of adsorption of biomolecules and pharmaceuticals on nanostructured metal surfaces. In this work, the adsorption of B-group vitamins pyridoxine, nicotinic acid, folic acid and riboflavin at electrochemically prepared gold and silver substrates was investigated using Fourier transform SERS spectroscopy at an excitation wavelength of 1,064 nm. Gold and silver substrates were prepared by cathodic reduction on massive platinum targets. In the case of gold substrates, oxidation-reduction cycles were applied to increase the enhancement factor of the gold surface. The SERS spectra of riboflavin, nicotinic acid, folic acid and pyridoxine adsorbed on silver substrates differ significantly from SERS spectra of these B-group vitamins adsorbed on gold substrates. The analysis of near-infrared-excited SERS spectra reveals that each of B-group vitamin investigated interacts with the gold surface via a different mechanism of adsorption to that with the silver surface. In the case of riboflavin adsorbed on silver substrate, the interpretation of surface-enhanced infrared absorption (SEIRA) spectra was also helpful in investigation of the adsorption mechanism.

  8. Controlled graphene oxide assembly on silver nanocube monolayers for SERS detection: dependence on nanocube packing procedure

    Directory of Open Access Journals (Sweden)

    Martina Banchelli

    2016-01-01

    Full Text Available Hybrid graphene oxide/silver nanocubes (GO/AgNCs arrays for surface-enhanced Raman spectroscopy (SERS applications were prepared by means of two procedures differing for the method used in the assembly of the silver nanocubes onto the surface: Langmuir–Blodgett (LB transfer and direct sequential physisorption of silver nanocubes (AgNCs. Adsorption of graphene oxide (GO flakes on the AgNC assemblies obtained with both procedures was monitored by quartz crystal microbalance (QCM technique as a function of GO bulk concentration. The experiment provided values of the adsorbed GO mass on the AgNC array and the GO saturation limit as well as the thickness and the viscoelastic properties of the GO film. Atomic force microscopy (AFM measurements of the resulting samples revealed that a similar surface coverage was achieved with both procedures but with a different distribution of silver nanoparticles. In the GO covered LB film, the AgNC distribution is characterized by densely packed regions alternating with empty surface areas. On the other hand, AgNCs are more homogeneously dispersed over the entire sensor surface when the nanocubes spontaneously adsorb from solution. In this case, the assembly results in less-packed silver nanostructures with higher inter-cube distance. For the two assembled substrates, AFM of silver nanocubes layers fully covered with GO revealed the presence of a homogeneous, flexible and smooth GO sheet folding over the silver nanocubes and extending onto the bare surface. Preliminary SERS experiments on adenine showed a higher SERS enhancement factor for GO on Langmuir–Blodgett films of AgNCs with respect to bare AgNC systems. Conversely, poor SERS enhancement for adenine resulted for GO-covered AgNCs obtained by spontaneous adsorption. This indicated that the assembly and packing of AgNCs obtained in this way, although more homogeneous over the substrate surface, is not as effective for SERS analysis.

  9. Surface enhanced Raman scattering (SERS) with biopolymer encapsulated silver nanosubstrates for rapid detection of foodborne pathogens.

    Science.gov (United States)

    Sundaram, Jaya; Park, Bosoon; Kwon, Yongkuk; Lawrence, Kurt C

    2013-10-01

    A biopolymer encapsulated with silver nanoparticles was prepared using silver nitrate, polyvinyl alcohol (PVA) solution, and trisodium citrate. It was deposited on a mica sheet to use as SERS substrate. Fresh cultures of Salmonella Typhimurium, Escherichia coli, Staphylococcus aureus and Listeria innocua were washed from chicken rinse and suspended in 10 ml of sterile deionized water. Approximately 5 μl of the bacterial suspensions was placed on the substrate individually and exposed to 785 nm HeNe laser excitation. SERS spectral data were recorded over the Raman shift between 400 and 1800 cm(-1) from 15 different spots on the substrate for each sample; and three replicates were done on each bacteria type. Principal component analysis (PCA) model was developed to classify foodborne bacteria types. PC1 identified 96% of the variation among the given bacteria specimen, and PC2 identified 3%, resulted in a total of 99% classification accuracy. Soft Independent Modeling of Class Analogies (SIMCA) of validation set gave an overall correct classification of 97%. Comparison of the SERS spectra of different types of gram-negative and gram-positive bacteria indicated that all of them have similar cell walls and cell membrane structures. Conversely, major differences were noted around the nucleic acid and amino acid structure information between 1200 cm(-1) and 1700 cm(-1) and at the finger print region between 400 cm(-1) and 700 cm(-1). Silver biopolymer nanoparticle substrate could be a promising SERS tool for pathogen detection. Also this study indicates that SERS technology could be used for reliable and rapid detection and classification of food borne pathogens. Published by Elsevier B.V.

  10. Porous Silicon Covered with Silver Nanoparticles as Surface-Enhanced Raman Scattering (SERS) Substrate for Ultra-Low Concentration Detection.

    Science.gov (United States)

    Kosović, Marin; Balarin, Maja; Ivanda, Mile; Đerek, Vedran; Marciuš, Marijan; Ristić, Mira; Gamulin, Ozren

    2015-12-01

    Microporous and macro-mesoporous silicon templates for surface-enhanced Raman scattering (SERS) substrates were produced by anodization of low doped p-type silicon wafers. By immersion plating in AgNO3, the templates were covered with silver metallic film consisting of different silver nanostructures. Scanning electron microscopy (SEM) micrographs of these SERS substrates showed diverse morphology with significant difference in an average size and size distribution of silver nanoparticles. Ultraviolet-visible-near-infrared (UV-Vis-NIR) reflection spectroscopy showed plasmonic absorption at 398 and 469 nm, which is in accordance with the SEM findings. The activity of the SERS substrates was tested using rhodamine 6G (R6G) dye molecules and 514.5 nm laser excitation. Contrary to the microporous silicon template, the SERS substrate prepared from macro-mesoporous silicon template showed significantly broader size distribution of irregular silver nanoparticles as well as localized surface plasmon resonance closer to excitation laser wavelength. Such silver morphology has high SERS sensitivity that enables ultralow concentration detection of R6G dye molecules up to 10(-15) M. To our knowledge, this is the lowest concentration detected of R6G dye molecules on porous silicon-based SERS substrates, which might even indicate possible single molecule detection.

  11. Humic acids-based one-step fabrication of SERS substrates for detection of polycyclic aromatic hydrocarbons.

    Science.gov (United States)

    Qu, Lu-Lu; Li, Yuan-Ting; Li, Da-Wei; Xue, Jin-Qun; Fossey, John S; Long, Yi-Tao

    2013-03-07

    A facile one-step approach to fabricate substrates for surface-enhanced Raman scattering (SERS) detection of polycyclic aromatic hydrocarbons (PAHs) was explored by reduction of silver nitrate with humic acids (HAs). This simple process readily delivers silver nanoparticles (Ag NPs) decorated with HAs (HAs-Ag NPs), and an average diameter of 50 nm. More importantly, it compares favorably to Ag NPs prepared by the usual sodium citrate method, HAs-Ag NPs show excellent SERS activity for PAHs and display a remarkable capacity to absorb aromatic molecules through presumed π-π stacking interactions. Furthermore, the HAs-Ag NPs displayed good SERS stability, possibly due to the fact that HAs form loose coils or networks around the nanoparticles thus preventing aggregation. The investigation of qualitative and quantitative detection of PAHs on HAs-Ag NPs indicate that different PAHs can be distinguished easily from their discriminant SERS peaks, and the SERS responses exhibited a linear dependence on PAH concentrations over two orders of magnitude, with tens of nM detection limits. In addition, the HAs-Ag NPs performed well in the multicomponent analysis of PAH mixtures by the SERS technique without pre-separation.

  12. Application of surface-enhanced Raman scattering (SERS) for the identification of anthraquinone dyes used in works of art

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Kui [ORNL; Leona, Marco [ORNL; Yan, Fei [ORNL; Wabuyele, Musundi B [ORNL; Vo Dinh, Tuan [ORNL

    2006-04-01

    Surface-enhanced Raman scattering (SERS) was investigated for applications in the analysis of anthraquinone dyes used in works of art. Two SERS procedures were developed and evaluated with three frequently used anthraquinone dyes, alizarin, carminic acid and lac dye. The first procedure involves coating a layer of silver nanoparticles directly on pieces of filter paper stained with the dyes of interest by thermal evaporation to induce SERS effect. In the second procedure, a SERS-active Ag-Al{sub 2}O{sub 3} substrate was prepared by spin-coating an alumina-nanoparticle layer onto a glass slide to provide the nanostructure of the substrate, followed by thermally evaporating a layer of silver nanoparticles on top of the alumina layer. Aliquots of dye solutions were delivered onto this substrate to be analyzed. Intense SERS spectra characteristic of alizarin, carminic acid and lac dye were obtained using both SERS procedures. The effects of two parameters, the concentration of the alumina suspension and the thickness of the silver nanoparticle layer on the performance of the Ag-Al{sub 2}O{sub 3} substrate were examined with alizarin as the model compound. Comparative studies were conducted between the Ag-Al{sub 2}O{sub 3} substrate and the SERS substrate prepared using Tollens reaction. The Ag-Al{sub 2}O{sub 3} substrate was shown to offer larger enhancement and improved reproducibility than the Tollens substrates. Finally, the potential applicability of the Ag-Al{sub 2}O{sub 3} substrate for the analysis of real artifact objects was illustrated by the identification of alizarin extracted from a small piece of textile dyed using traditional methods and materials. The limit of detection for alizarin was estimated to be 7 x 10{sup -15} g from tests performed on solutions of known concentration.

  13. Uranium industry annual 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-04-01

    The Uranium Industry Annual 1996 (UIA 1996) provides current statistical data on the US uranium industry`s activities relating to uranium raw materials and uranium marketing. The UIA 1996 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. Data on uranium raw materials activities for 1987 through 1996 including exploration activities and expenditures, EIA-estimated reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities for 1994 through 2006, including purchases of uranium and enrichment services, enrichment feed deliveries, uranium fuel assemblies, filled and unfilled market requirements, uranium imports and exports, and uranium inventories are shown in Chapter 2. A feature article, The Role of Thorium in Nuclear Energy, is included. 24 figs., 56 tabs.

  14. Electric power annual 1992

    Energy Technology Data Exchange (ETDEWEB)

    1994-01-06

    The Electric Power Annual presents a summary of electric utility statistics at national, regional and State levels. The objective of the publication is to provide industry decisionmakers, government policymakers, analysts and the general public with historical data that may be used in understanding US electricity markets. The Electric Power Annual is prepared by the Survey Management Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); US Department of Energy. ``The US Electric Power Industry at a Glance`` section presents a profile of the electric power industry ownership and performance, and a review of key statistics for the year. Subsequent sections present data on generating capability, including proposed capability additions; net generation; fossil-fuel statistics; retail sales; revenue; financial statistics; environmental statistics; electric power transactions; demand-side management; and nonutility power producers. In addition, the appendices provide supplemental data on major disturbances and unusual occurrences in US electricity power systems. Each section contains related text and tables and refers the reader to the appropriate publication that contains more detailed data on the subject matter. Monetary values in this publication are expressed in nominal terms.

  15. Detection and identification of Huo-Xue-Hua-Yu decoction (HXHYD) using surface-enhanced Raman scattering (SERS) spectroscopy and multivariate analysis

    Science.gov (United States)

    Chen, Weiwei; Lin, Jia; Chen, Rong; Feng, Shangyuan; Yu, Yun; Lin, Duo; Huang, Meizhen; Shi, Hong; Huang, Hao

    2015-04-01

    We have evaluated the capabilities of surface-enhanced Raman scattering (SERS) technology for analyzing two Huo-Xue-Hua-Yu decoctions (HXHYDs) prepared according to different prescriptions. The aim of this study was to evaluate the relevance of SERS technology applied to decoction of traditional Chinese medicines (TCM). HXHYD I was prepared according to the original prescription; the same preparation method was used for the HXHYD II, except for the crudeweight ratio described in the original prescription. There was no Raman signal in conventional Raman spectra of HXHYDs. Silver nanoparticles were directly mixed with HXHYDs to enhance the Raman scattering of biochemical constituents, and high quality SERS spectra were obtained. Significant differences in SERS spectra between HXHYD I and II can be observed, which showed special changes in the percentage of biochemical constituents in different decoctions. Principal components analysis (PCA) combined with linear discriminant analysis (LDA) were employed to generate diagnostic algorithms for classification of SERS spectra of two HXHYDs, and showed that a diagnostic accuracy of 100% can be achieved. This work demonstrated that the SERS technique has potential for spectral characteristic detection for decoction of TCM with high sensitivity, and that this technique, combined with PCA-LDA, can be used for quality control of the extracted decoction of TCM and production management of Chinese herbal preparations.

  16. Evaluation of SERS substrates for chemical agent detection

    Science.gov (United States)

    Huang, Hermes; Shende, Chetan; Sengupta, Atanu; Inscore, Frank; Farquharson, Stuart

    2012-06-01

    US Military forces are dependent on indigenous water supplies, which are considered prime targets to effect a chemical or biological attack. Consequently, there is a clear need for a portable analyzer capable of evaluating water supplies prior to use. To this end we have been investigating the use of a portable Raman analyzer with surface-enhanced Raman spectroscopy (SERS) sampling systems. The superior selectivity and exceptional sensitivity of SERS has been demonstrated by the detection of single molecules. However, the extreme sensitivity provided by SERS is attributed to "hot spot" structures, such as particle junctions that can provide as much as 10 orders of magnitude enhancement. Unfortunately, hotspots are not evenly distributed across substrates, which results in enhancements that cannot be quantitatively reproduced. Here we present analysis of uniformity for a newly developed substrate and commercial sample vials using benzenethiol and bispyridylethylene, two chemicals often used to characterize SERS substrates, and methyl phosphonic acid, a major hydrolysis product of the nerve agents.

  17. Development of a SERS aptasensor for detection of medical residues

    DEFF Research Database (Denmark)

    Frøhling, Kasper Bayer

    of stringency successful detection was never accomplished. In conclusion, this PhD project successfully characterised the chemical functionalisation parameters needed for generic SERS aptasensor development using only the Raman signals of the molecules. The SERS substrate was successfully fabricated repeatedly......Low levels of medical residues in environmental, industrial and domestic water systems is a growing concern. The biosensor industry is trying to accomodate the need of sensitive and specific sensor systems capable of ultra-low level detection of medical residues. In this PhD project a surface...... enhanced Raman spectroscopy (SERS) sensor for the female sexhormone 17β-estradiol was attempted. It is commonly used in contraceptive pills from where it find its way through waste water treatment plants and into the environment. The SERS substrate was fabricated in a cleanroom facility using techniques...

  18. SERS diagnostic platforms, methods and systems microarrays, biosensors and biochips

    Energy Technology Data Exchange (ETDEWEB)

    Vo-Dinh, Tuan (Knoxville, TN)

    2007-09-11

    A Raman integrated sensor system for the detection of targets including biotargets includes at least one sampling platform, at least one receptor probe disposed on the sampling platform, and an integrated circuit detector system communicably connected to the receptor. The sampling platform is preferably a Raman active surface-enhanced scattering (SERS) platform, wherein the Raman sensor is a SERS sensor. The receptors can include at least one protein receptor and at least one nucleic acid receptor.

  19. SERS diagnostic platforms, methods and systems microarrays, biosensors and biochips

    Science.gov (United States)

    Vo-Dinh, Tuan

    2007-09-11

    A Raman integrated sensor system for the detection of targets including biotargets includes at least one sampling platform, at least one receptor probe disposed on the sampling platform, and an integrated circuit detector system communicably connected to the receptor. The sampling platform is preferably a Raman active surface-enhanced scattering (SERS) platform, wherein the Raman sensor is a SERS sensor. The receptors can include at least one protein receptor and at least one nucleic acid receptor.

  20. Annual report

    Energy Technology Data Exchange (ETDEWEB)

    Barkas, W.H.

    1967-05-01

    Work that has been carried out in several areas of High Energy Physics is described. Visual techniques are used. For the study of the three-pion decay of the K/sup +/ meson, bubble chamber and emulsion methods are used to complement each other, particularly for the tau' mode. Several separate bubble chamber experiments in ..pi../sup +/p, anti pp and K/sup -/p interactions are also described, and the large effort devoted to computer reprogramming for the data analysis indicated. Two emulsion research projects are also described which have for their purpose the study of electromagnetic processes induced by 16 BeV electrons and the study of hypernuclei by K/sup -/ mesons. For the latter work high speed computer methods have been successfully applied to the analysis of the product hypernuclei. These programs of work have not been under way long enough for the main results yet to be available, but some preliminary data have been published and several reports on data analysis and related subjects have been prepared.

  1. Superhydrophobic Ag nanostructures on polyaniline membranes with strong SERS enhancement.

    Science.gov (United States)

    Liu, Weiyu; Miao, Peng; Xiong, Lu; Du, Yunchen; Han, Xijiang; Xu, Ping

    2014-11-07

    We demonstrate here a facile fabrication of n-dodecyl mercaptan-modified superhydrophobic Ag nanostructures on polyaniline membranes for molecular detection based on SERS technique, which combines the superhydrophobic condensation effect and the high enhancement factor. It is calculated that the as-fabricated superhydrophobic substrate can exhibit a 21-fold stronger molecular condensation, and thus further amplifies the SERS signal to achieve more sensitive detection. The detection limit of the target molecule, methylene blue (MB), on this superhydrophobic substrate can be 1 order of magnitude higher than that on the hydrophilic substrate. With high reproducibility, the feasibility of using this SERS-active superhydrophobic substrate for quantitative molecular detection is explored. A partial least squares (PLS) model was established for the quantification of MB by SERS, with correlation coefficient R(2) = 95.1% and root-mean-squared error of prediction (RMSEP) = 0.226. We believe this superhydrophobic SERS substrate can be widely used in trace analysis due to its facile fabrication, high signal reproducibility and promising SERS performance.

  2. The Importance and Usefulness of Corporate Annual Reports in Malaysia

    OpenAIRE

    Mohd. Ghazali, Nazli Anum

    2010-01-01

    The purpose of this study is to examine the perceived importance and usefulness of corporate annual reports in Malaysia and to determine if there is a difference between the perceptions of preparers and users of corporate annual reports. Data for this study were collected using a questionnaire survey conducted in late 2006 and early 2007. Preparers are represented by chief financial officers while users by investment analysts. The mean scores for preparers and users are compared using the ind...

  3. Biocompatible 3D SERS substrate for trace detection of amino acids and melamine.

    Science.gov (United States)

    Satheeshkumar, Elumalai; Karuppaiya, Palaniyandi; Sivashanmugan, Kundan; Chao, Wei-Ting; Tsay, Hsin-Sheng; Yoshimura, Masahiro

    2017-03-21

    A novel, low-cost and biocompatible three-dimensional (3D) substrate for surface-enhanced Raman spectroscopy (SERS) is fabricated using gold nanoparticles (AuNPs) loaded on cellulose paper for detection of amino acids and melamine. Dysosma pleiantha rhizome (Dp-Rhi) capped AuNPs (Dp-Rhi_AuNPs) were prepared by in situ using aqueous extract of Dp-Rhi and in situ functionalized Dp-Rhi on AuNPs surface was verified by Fourier transform infrared spectroscopy and zeta potentials analysis shows a negative (-18.4mV) surface charges, which confirm that presence of Dp-Rhi on AuNPs. The biocompatibility of Dp-Rhi_AuNPs is also examined by cell viability of FaDu cells using MTS assay and compared to control group. In conclusion, the SERS performance of AuNPs@cellulose paper substrates were systematically demonstrated and examined with different excitation wavelengths (i.e. 532, 632.8 and 785nm lasers) and the as-prepared 3D substrates provided an enhancement factor approaching 7 orders of magnitude compared with conventional Raman intensity using para-nitrothiophenol (p-NTP), para-aminothiophenol (p-ATP) and para-mercaptobenzoic acid (p-MBA) as probe molecules. The strong electromagnetic effect was generated at the interface of AuNPs and pre-treated roughened cellulose paper is also investigated by simulation in which the formation of possible Raman hot-spot zone in fiber-like microstructure of cellulose paper decorated with AuNPs. Notably, with optimized condition of as-prepared 3D AuNPs@cellulose paper is highly sensitive in the SERS detection of aqueous tyrosine (10(-10)M) and melamine (10(-9)M).

  4. SERS substrates for in-situ biosensing (Conference Presentation)

    Science.gov (United States)

    Venugopalan, Priyamvada; Quilis, Nestor; Jakub, Dostalek; Wolfgang, Knoll

    2017-06-01

    Abstract: Recent years have seen a rapid progress in the field of surface-enhanced Raman spectroscopy (SERS) which is attributed to the thriving field of plasmonics [1]. SERS is a susceptible technique that can address basic scientific questions and technological problems. In both cases, it is highly dependent upon the plasmonic substrate, where excitation of the localized surface plasmon resonance enhances the vibrational scattering signal of the analyte molecules adsorbed on to the surface [2]. In this work, using finite difference time domain (FDTD) method we investigate the optical properties of plasmonic nanostructures with tuned plasmonic resonances as a function of dielectric environment and geometric parameters. An optimized geometry will be discussed based on the plasmonic resonant position and the SERS intensity. These SERS substrates will be employed for the detection of changes in conformation caused by interactions between an aptamer and analyte molecules. This will be done by using a microfluidic channel designed within the configuration of the lab-on-a-chip concept based on the intensity changes of the SERS signal. More efficient and reproducible results are obtained for such a quantitative measurement of analytes at low concentration levels. We will also demonstrate that the plasmonic substrates fabricated by top down approach such as e-beam lithography (EBL) and laser interference lithography (LIL) are highly reproducible, robust and can result in high electric field enhancement. Our results demonstrate the potential to use SERS substrates for highly sensitive detection schemes opening up the window for a wide range of applications including biomedical diagnostics, forensic investigation etc. Acknowledgement: This work was supported by the Austrian Science Fund (FWF), project NANOBIOSENSOR (I 2647). References: [1] J. N. Anker, W. P. Hall, O. Lyandres, N. C. Shah, J. Zhao and R. P. V. Duyne., " Biosensing with plasmonic nanosensors," Nature

  5. Flower-shaped gold nanoparticles: synthesis, characterization and their application as SERS-active tags inside living cells

    Energy Technology Data Exchange (ETDEWEB)

    Boca, Sanda; Astilean, Simion [Nanobiophotonics Center, Institute for Interdisciplinary Research in Nanobioscience, Babes-Bolyai University, Treboniu Laurian 42, 400271 Cluj-Napoca (Romania); Rugina, Dumitrita; Pintea, Adela [Department of Biochemistry, University of Agricultural Sciences and Veterinary Medicine, Manastur 3-5, 400372, Cluj-Napoca (Romania); Barbu-Tudoran, Lucian, E-mail: sanda.boca@phys.ubbcluj.ro, E-mail: simion.astilean@phys.ubbcluj.ro [Electron Microscopy Center, Faculty of Biology and Geology, Babes-Bolyai University, Clinicilor 5-7, 400006, Cluj-Napoca (Romania)

    2011-02-04

    The detection of Raman signals inside living cells is a topic of great interest in the study of cell biology mechanisms and for diagnostic and therapeutic applications. This work presents the synthesis and characterization of flower-shaped gold nanoparticles and demonstrates their applicability as SERS-active tags for cellular spectral detection. The particles were synthesized by a facile, rapid new route that uses ascorbic acid as a reducing agent of gold salt. Two triarylmethane dyes which are widely used as biological stains, namely malachite green oxalate and basic fuchsin, were used as Raman-active molecules and the polymer mPEG-SH as capping material. The as-prepared SERS-active nanoparticles were tested on a human retinal pigment epithelial cell line and found to present a low level of cytotoxicity and high chemical stability together with SERS sensitivity down to picomolar particle concentrations.

  6. Flower-shaped gold nanoparticles: synthesis, characterization and their application as SERS-active tags inside living cells

    Science.gov (United States)

    Boca, Sanda; Rugina, Dumitrita; Pintea, Adela; Barbu-Tudoran, Lucian; Astilean, Simion

    2011-02-01

    The detection of Raman signals inside living cells is a topic of great interest in the study of cell biology mechanisms and for diagnostic and therapeutic applications. This work presents the synthesis and characterization of flower-shaped gold nanoparticles and demonstrates their applicability as SERS-active tags for cellular spectral detection. The particles were synthesized by a facile, rapid new route that uses ascorbic acid as a reducing agent of gold salt. Two triarylmethane dyes which are widely used as biological stains, namely malachite green oxalate and basic fuchsin, were used as Raman-active molecules and the polymer mPEG-SH as capping material. The as-prepared SERS-active nanoparticles were tested on a human retinal pigment epithelial cell line and found to present a low level of cytotoxicity and high chemical stability together with SERS sensitivity down to picomolar particle concentrations.

  7. SERS and quantum chemical studies on N-methylglycine molecule on silver nanoparticles

    Science.gov (United States)

    Parameswari, A.; Mohamed Asath, R.; Premkumar, R.; Milton Franklin Benial, A.

    2017-06-01

    Adsorption characteristics of N-methylglycine (Sar) on silver surface was investigated based on density functional theory and surface enhanced Raman spectroscopy (SERS) technique. The Sar was characterized by powder X-ray diffraction (XRD) and dynamic light scattering techniques. AgNPs were prepared by solution combustion method using urea as fuel. The AgNPs were characterized by XRD and high-resolution transmission electron microscopic techniques. The structural parameters of Sar molecule were compared with the reported experimental XRD values. The change in structural parameters of Sar after adsorption on silver surface shows the interaction between Sar and silver cluster. Raman frequencies were calculated for Sar, Sar adsorbed on silver surface and their corresponding spectra were studied experimentally. The conventional Raman and SERS frequencies were compared with the experimental values and analyzed on the basis of potential energy distribution calculation. Natural bond orbital analysis was carried out to investigate the intra-molecular stabilization interactions, which confirms the bioactivity of the molecule. Frontier molecular orbital analysis was carried out for Sar and Sar adsorbed on silver surface. The reduced band gap value was observed for Sar after adsorption on silver surface. The reduction in band gap indicates the intermolecular charge transfer, which leads to the bioactivity of the title molecule. SERS spectral analysis shows that the Sar adsorbed as stand-on orientation on the silver surface.

  8. One-step Solution Processing of Ag, Au and Pd@MXene Hybrids for SERS

    Science.gov (United States)

    Satheeshkumar, Elumalai; Makaryan, Taron; Melikyan, Armen; Minassian, Hayk; Gogotsi, Yury; Yoshimura, Masahiro

    2016-08-01

    We report on one-step hybridization of silver, gold and palladium nanoparticles from solution onto exfoliated two-dimensional (2D) Ti3C2 titanium carbide (MXene) nanosheets. The produced hybrid materials can be used as substrates for surface-enhanced Raman spectroscopy (SERS). An approximate analytical approach is also developed for the calculation of the surface plasmon resonance (SPR) frequency of nanoparticles immersed in a medium, near the interface of two dielectric media with different dielectric constants. We obtained a good match with the experimental data for SPR wavelengths, 440 nm and 558 nm, respectively for silver and gold nanoparticles. In the case of palladium, our calculated SPR wavelength for the planar geometry was 160 nm, demonstrating that non-spherical palladium nanoparticles coupled with 2D MXene yield a broad, significanlty red-shifted SPR band with a peak at 230 nm. We propose a possible mechanism of the plasmonic hybridization of nanoparticles with MXene. The as-prepared noble metal nanoparticles on MXene show a highly sensitive SERS detection of methylene blue (MB) with calculated enhancement factors on the order of 105. These findings open a pathway for extending visible-range SERS applications of novel 2D hybrid materials in sensors, catalysis, and biomedical applications.

  9. Large area flexible SERS active substrates using engineered nanostructures

    Science.gov (United States)

    Chung, Aram J.; Huh, Yun Suk; Erickson, David

    2011-07-01

    Surface enhanced Raman scattering (SERS) is an analytical sensing method that provides label-free detection, molecularly specific information, and extremely high sensitivity. The Raman enhancement that makes this method attractive is mainly attributed to the local amplification of the incident electromagnetic field that occurs when a surface plasmon mode is excited at a metallic nanostructure. Here, we present a simple, cost effective method for creating flexible, large area SERS-active substrates using a new technique we call shadow mask assisted evaporation (SMAE). The advantage of large, flexible SERS substrates such as these is they have more area for multiplexing and can be incorporated into irregular surfaces such as clothing. We demonstrate the formation of four different types of nanostructure arrays (pillar, nib, ellipsoidal cylinder, and triangular tip) by controlling the evaporation angle, substrate rotation, and deposition rate of metals onto anodized alumina nanoporous membranes as large as 27 mm. In addition, we present experimental results showing how a hybrid structure comprising of gold nanospheres embedded in a silver nano-pillar structure can be used to obtain a 50× SERS enhancement over the raw nanoparticles themselves.Surface enhanced Raman scattering (SERS) is an analytical sensing method that provides label-free detection, molecularly specific information, and extremely high sensitivity. The Raman enhancement that makes this method attractive is mainly attributed to the local amplification of the incident electromagnetic field that occurs when a surface plasmon mode is excited at a metallic nanostructure. Here, we present a simple, cost effective method for creating flexible, large area SERS-active substrates using a new technique we call shadow mask assisted evaporation (SMAE). The advantage of large, flexible SERS substrates such as these is they have more area for multiplexing and can be incorporated into irregular surfaces such as

  10. Gold nanoparticles paper as a SERS bio-diagnostic platform.

    Science.gov (United States)

    Ngo, Ying Hui; Then, Whui Lyn; Shen, Wei; Garnier, Gil

    2013-11-01

    Bioactive papers are usually challenged by four major limitations: sensitivity, selectivity, simplicity and strength (4S). Gold nanoparticles (AuNPs) treated paper has previously been demonstrated as a Surface Enhanced Raman Scattering (SERS) active substrate, capable of addressing the 4S issues. In this study, AuNPs on paper substrate were functionalized by a series of biomolecules to develop a generic SERS platform for antibody-antigen detection. The functionalization steps were performed by taking advantage of the high affinity association between Streptomyces avidinii-derived protein, streptavidin, and biotin. Streptavidin was firstly bound onto the AuNPs treated paper using biotinylated-thiol. Subsequently, desired biotinylated-antibody was bound onto the streptavidin. SERS spectra of each functionalization step were obtained to ensure specific adsorption of the bio-molecules. The binding interaction of the antibody with its specific antigen was detected using SERS. Shifts of Raman band associated with α-helix and β-sheet structures indicated structural modification of the antibody upon interaction with its antigen. Predominant tryptophan and tyrosine residue bands were also detected, confirming the presence of antigen. Reproducible spectral features were quantified as AuNP papers were subjected to different concentrations of antigen; the spectra intensity increased as a function of the antigen concentration. The retention of AuNPs on paper remained constant after all the consecutive washing and functionalization steps. The feasibility of AuNPs paper as a low-cost and generic SERS platform for bio-diagnostic applications was demonstrated.

  11. Super-SERS-active and highly effective antimicrobial Ag nanodendrites

    Science.gov (United States)

    Li, H. B.; Liu, P.; Liang, Y.; Xiao, J.; Yang, G. W.

    2012-07-01

    We have developed simple and green electrochemistry to synthesize Ag nanostructures with high purity, good crystallinity and smooth surface for applications as super-SERS (surface-enhanced Raman scattering), SERS-active substrates and with highly effective antimicrobial activities. This synthesis takes place in a clean and slow reaction environment without any chemical additives, which ensures an ultrahigh active surface of the as-synthesized Ag nanostructures owing to their purity, good crystallinity and smooth morphology. Using this method, we synthesized nearly perfect Ag nanodendrites (NDs), which exhibit super-SERS sensitivity when they are used to detect the SERS spectra of rhodamine 6G at concentrations as low as 5 × 10-16 M, and have an ultrahigh electromagnetic (EM) enhancement factor of the order of 1013, breaking through the theoretical limit of EM enhancement. Meanwhile, the as-synthesized Ag NDs possess highly effective antimicrobial activities for Escherichia coli, Candida albicans and Staphylococcus aureus, which are over 10 times that of silver nanoparticles. Additionally, the basic physics and chemistry involved in the fabrication of Ag nanostructures are pursued. These investigations show that silver nanostructures with highly active surfaces can make the most of Ag nanostructures functioning as super-SERS-active substrates and multiple antibiotics.

  12. LA GENÉTICA Y LA DIGNIDAD DEL SER HUMANO

    Directory of Open Access Journals (Sweden)

    Nicolás Jouve de Barreda

    2013-01-01

    Full Text Available Los elementos biológicos del hombre no son suficientes para afrontar las cuestiones bioéticas que se plantean en torno al concepto de persona, pero son necesarios para definir con precisión las propiedades de los seres humanos y los aspectos teológicos, filosóficos y jurídicos que son atribuibles a cada persona. El ser humano es un ser singular. En él que conviven dos dimensiones de distinta naturaleza, una material y otra espiritual, y precisamente esta es la principal de las diferencias entre el hombre y el resto de seres vivos. Pero además en el hombre aparece una nueva característica, única entre los seres vivos, la componente ética. Los valores y pautas del comportamiento moral y ético del ser humano deben considerarse de origen natural ya que han contribuido al éxito y supervivencia de la especie. El hombre no es solo Homo sapiens sino también Homo moralis. El reconocimiento de culpa, autodominio, solidaridad, amor, generosidad, altruismo y honestidad, entre otras, son cualidades innatas en los seres humanos. La antropología filosófica destaca el carácter único e irrepetible propio de cada ser humano, cada persona. Esto coincide plenamente con los datos de la ciencia, que destaca la identidad genética individual y singular de cada ser humano.

  13. Normal Raman and SERS spectroscopy of the vitamin E

    Energy Technology Data Exchange (ETDEWEB)

    Cai Tiantian; Gu Huaimin; Yuan Xiaojuan; Liu Fangfang, E-mail: guhm@scnu.edu.cn [MOE Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, 510631, Guangzhou (China)

    2011-01-01

    In this study, surface-enhanced Raman scattering(SERS)spectra of vitamin E were obtained on colloidal silver(Ag). Alpha-(-) tocopherol which is the only form that is recognized to meet human requirements was selected to study. The analytes ({+-})- -tocopherol were dissolved in chloroform (CHCl{sub 3}) and the silver colloid was poured into the compound. Silver colloid was reduced by hydroxylamine hydrochloride. The analytes were the supernatant after standing the mixture for the reason that chloroform have no signals in surface-enhanced Raman scattering in the Ag colloid, and it would not affect the determination of the ({+-})- -tocopherol. The Normal Raman and SERS spectrum of Vitamin E were contrastively studied to realize how the vitamin E stuck to the silver nanoparticles. The results show the fat-soluble substances can be analysed by SERS. The spectra indicate that the molecules are adsorbed on the surface through the COO- groups by the simultaneous involvement of a and -type coordination. These results suggest some important criteria for consideration in SERS measurements and also provide important insights into the problem of predicting SERS activities for different fat-soluble substances.

  14. Combined SPR and SERS: Otto and Kretschmann configurations

    Science.gov (United States)

    Barchiesi, Dominique; Grosges, Thomas; Colas, Florent; Lamy de la Chapelle, Marc

    2015-11-01

    Combined surface enhanced Raman spectroscopy (SERS) and surface plasmon resonance (SPR) setup was proposed recently. The experimental setup requires a gold layer deposited on a glass substrate. However, due to the poor ability of sticking gold material on glass, a nanometric adhesion layer is used. In this paper we compare numerically both the SERS gain, and the SPR signal, for metallic and dielectric adhesion layers, for two substrates. We show that even if the dielectric materials can be considered as equivalent for the SPR signal, this is not the case for the SERS gain. In particular the dielectric adhesion layer reduces the sensitivity of the SERS gain to this parameter and therefore their use could be more suitable for the fabrication of the sensor. Moreover the higher the refractive index of substrate with regards to the adhesion layer is, the higher efficiency of setup is obtained, and therefore the Otto configuration seems to be more efficient than the Kretschmann one. Optimization of the thicknesses of the adhesion layer and of the gold layer can lead to a SERS gain greater than 103 without nanostructuring.

  15. PromoSer: improvements to the algorithm, visualization and accessibility.

    Science.gov (United States)

    Halees, Anason S; Weng, Zhiping

    2004-07-01

    PromoSer is a web service that provides an easy and efficient approach to the batch retrieval of a large number of proximal promoters. Since its introduction last year, it has undergone continued development and expansion. At the core, there have been improvements in the filtering of the raw mRNA/EST sequences upon which all predictions are built, improvements in the alignments clustering and transcription start site prediction algorithms, and improvements in the backing database for increased performance. At the user interface level, improvements include enhanced functionality and user options, better integration with other resources on the web and a new visualization tool. PromoSer now also supports queries using a SOAP-based interface and XML-based responses. The service is publicly available at http://biowulf.bu.edu/zlab/PromoSer.

  16. ser en otorrinolaringología

    OpenAIRE

    Castillo F,Marcela; Lanas V,Andrés

    2006-01-01

    Esta revisión bibliográfica analiza los diferentes tipos de láser usados en otorrinolaringología, destacando las propiedades de cada uno. Posteriormente describe las características de una sesión con anestesia y uso de láser y las patologías con indicaciones de cirugía láser. Estas se clasifican en cinco rubros. Patología laringotraqueal, patología faringobucal, patología sinusal, otología y dermatología cérvico facial. En la primera se analizan: lesiones fonoquirúrgico, papilomatosis larínge...

  17. LA ÍNDOLE TECNOLÓGICA DEL SER HUMANO

    Directory of Open Access Journals (Sweden)

    José Luis del Barco Collazos

    2009-01-01

    Full Text Available La técnica no es accidental, sino esencial, al ser humano. Es el modo de vida del homo sapiens sapiens. No ha habido vida humana pretecnológica ni un periodo de la historia sin ella. Desde que el hombre puso los pies sobre la tierra empezó a fabricar instrumentos. Y seguirá fabricándolos. El arado, la aeronave y los ingenios técnicos del futuro son fases del progreso técnico. La asombrosa capacidad humana para fabricar se debe a la conexión entre las manos y el cerebro. La técnica no debe ser objeto de reverencia ni de temor pero sí debe ser gobernada.

  18. ser en otorrinolaringología

    OpenAIRE

    Castillo F,Marcela; Lanas V,Andrés

    2006-01-01

    Esta revisión bibliográfica analiza los diferentes tipos de láser usados en otorrinolaringología, destacando las propiedades de cada uno. Posteriormente describe las características de una sesión con anestesia y uso de láser y las patologías con indicaciones de cirugía láser. Estas se clasifican en cinco rubros. Patología laringotraqueal, patología faringobucal, patología sinusal, otología y dermatología cérvico facial. En la primera se analizan: lesiones fonoquirúrgico, papilomatosis larínge...

  19. Intracellular surface-enhanced Raman scattering (SERS) with thermally stable gold nanoflowers grown from Pt and Pd seeds

    KAUST Repository

    Song, Hyon Min

    2013-01-01

    SERS provides great sensitivity at low concentrations of analytes. SERS combined with near infrared (NIR)-resonant gold nanomaterials are important candidates for theranostic agents due to their combined extinction properties and sensing abilities stemming from the deep penetration of laser light in the NIR region. Here, highly branched gold nanoflowers (GNFs) grown from Pd and Pt seeds are prepared and their SERS properties are studied. The growth was performed at 80°C without stirring, and this high temperature growth method is assumed to provide great shape stability of sharp tips in GNFs. We found that seed size must be large enough (>30 nm in diameter) to induce the growth of those SERS-active and thermally stable GNFs. We also found that the addition of silver nitrate (AgNO3) is important to induce sharp tip growth and shape stability. Incubation with Hela cells indicates that GNFs are taken up and reside in the cytoplasm. SERS was observed in those cells incubated with 1,10-phenanthroline (Phen)-loaded GNFs. This journal is © 2013 The Royal Society of Chemistry.

  20. Effective SERS-active substrates composed of hierarchical micro/nanostructured arrays based on reactive ion etching and colloidal masks

    Science.gov (United States)

    Zhang, Honghua; Liu, Dilong; Hang, Lifeng; Li, Xinyang; Liu, Guangqiang; Cai, Weiping; Li, Yue

    2016-09-01

    A facile route has been proposed for the fabrication of morphology-controlled periodic SiO2 hierarchical micro/nanostructured arrays by reactive ion etching (RIE) using monolayer colloidal crystals as masks. By effectively controlling the experimental conditions of RIE, the morphology of a periodic SiO2 hierarchical micro/nanostructured array could be tuned from a dome-shaped one to a circular truncated cone, and finally to a circular cone. After coating a silver thin layer, these periodic micro/nanostructured arrays were used as surface-enhanced Raman scattering (SERS)-active substrates and demonstrated obvious SERS signals of 4-Aminothiophenol (4-ATP). In addition, the circular cone arrays displayed better SERS enhancement than those of the dome-shaped and circular truncated cone arrays due to the rougher surface caused by physical bombardment. After optimization of the circular cone arrays with different periodicities, an array with the periodicity of 350 nm exhibits much stronger SERS enhancement and possesses a low detection limit of 10-10 M 4-ATP. This offers a practical platform to conveniently prepare SERS-active substrates.

  1. Incorporation of multilayered silver nanoparticles into polymer brushes as 3-dimensional SERS substrates and their application for bacteria detection

    Science.gov (United States)

    Zhang, Qian; Wang, Xiang-Dong; Tian, Ting; Chu, Li-Qiang

    2017-06-01

    Surface-enhanced Raman scattering (SERS) sensors have been extensively studied for ultrasensitive detection of diverse chemical or biological analytes. Facile fabrication of highly sensitive SERS substrates is believed to be of crucial importance in these analytical applications. In this regard, the preparation of 3-dimensional (3D) SERS substrates are explored via the incorporation of multilayered silver nanoparticles (AgNPs) into poly (oligo(ethylene glycol) methacrylate) (POEGMA) brushes by repeating the immersion-rinsing-drying steps for different lengths of time (i.e., the so-called in-stacking method). The POEGMA brushes of different chain lengths are synthesized by surface-initiated atom transfer radical polymerization (ATRP) with various reaction time. The resulting POEGMA/AgNP nanocomposites are characterized by FE-SEM, UV-vis and Raman spectroscopy. FE-SEM and UV-vis results indicate that the AgNPs are successfully incorporated into the POEGMA brushes with a 3D configuration. The nanocomposite films are employed as SERS substrates for the detection of a Raman reporter molecule (i.e., 4-aminothiophenol), giving rise to an enhancement factor of up to 1.29 × 107 and also having relatively good uniformity and reproducibility. The obtained 3D SERS substrates are also used for the detection of a typical gram-positive bacterium, Staphylococcus aureus. The limit of detection is found to be as low as ca. 8 CFU/mL.

  2. Towards SERS based applications in food analytics: Lipophilic sensor layers for the detection of Sudan III in food matrices

    Energy Technology Data Exchange (ETDEWEB)

    Jahn, Martin; Patze, Sophie [Friedrich-Schiller University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena (Germany); Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Strasse 9, 07745 Jena (Germany); Bocklitz, Thomas [Friedrich-Schiller University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena (Germany); Weber, Karina [Friedrich-Schiller University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena (Germany); Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Strasse 9, 07745 Jena (Germany); Cialla-May, Dana, E-mail: dana.cialla-may@uni-jena.de [Friedrich-Schiller University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena (Germany); Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Strasse 9, 07745 Jena (Germany); Popp, Jürgen [Friedrich-Schiller University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena (Germany); Leibniz Institute of Photonic Technology (IPHT) Jena, Albert-Einstein-Strasse 9, 07745 Jena (Germany)

    2015-02-20

    Highlights: • A lipophilic sensor layer was applied to enzymatically grown SERS substrates. • Sudan III molecules could be detected in presence of water-insoluble competitors. • The carcinogenic food dye Sudan III was detected in a relevant concentration range. • Multivariate statistics allows quantitative measurements of Sudan III. • Sudan III contaminations were successfully detected out of spiked paprika powder. - Abstract: Food safety is a topic of great importance for our society which places high demands on analytical methods. Surface enhanced Raman spectroscopy (SERS) meets the requirements for a rapid, sensitive and specific detection technique. The fact that metallic colloids, one of the most often used SERS substrates, are usually prepared in aqueous solution makes the detection of water-insoluble substances challenging. In this paper we present a SERS based approach for the detection of water-insoluble molecules by applying a hydrophobic surface modification onto the surface of enzymatic generated silver nanoparticles. By this approach the detection of the illegal water-insoluble food dyes, such as Sudan III in presence of riboflavin, as water-soluble competitor, is possible. Moreover, we demonstrate the usability of this kind of SERS substrates for determination of Sudan III out of spiked paprika extracts.

  3. SER Y ESTAR CON ADJETIVOS – SIGNIFICACION DE LAS PROPOSICIONES SER Y ESTAR CON ADJETIVOS – SIGNIFICACION DE LAS PROPOSICIONES

    Directory of Open Access Journals (Sweden)

    Javier García de María

    2008-07-01

    Full Text Available Ser y estar: uno de los temas difíciles de la enseñanza/aprendizaje del español. Este trabajo expone un enfoque que recurre a la significación como hilo conductor. Por una parte, a la significación de ser y de estar como verbos, en sí mismos. Por otra, a la significación de los adjetivos. El planteamiento no es ya qué significa un determinado adjetivo con ser o con estar, sino cuál de estos verbos puede expresar su significado único o cada uno de sus significados. Sobre esta base el análisis considera, primero, los significados de ser/estar y los significados de un adjetivo dado y, segundo, los resultados significativos que arroja la relación entre verbos y adjetivo. Cuando el significado de un adjetivo se pueda expresar con los dos verbos, la elección vendrá determinada por la intencionalidad del hablante en el contexto comunicativo en que se encuentre. A partir de aquí las construcciones en las que aparecen ser y estar son tratadas como proposiciones retórico-argumentativas.Ser and estar: a difficult aspect of Spanish as a foreign language. This essay presents an approach that takes signification as guide line. On the one hand, the signification of ser and estar for their own cause; on the other hand the signification of the adjectives. The question is no longer what the meaning of a given adjective is with ser or with estar, but with which of the two verbs is it possible to express the unique meaning or each of the meanings of that adjective. Starting from this basis the approach considers first, the isolated meanings of the verbs and of the adjectives and, second, the resultant signification out of the relation between the two sides. If the meaning of a given adjective can be expressed by both verbs, the selection of the verb is determined by the intentionality of the speaker in the communicative context in which he negotiates. From this background on the syntactical constructions in which ser and estar appear are treated as

  4. Investigation of the catalysis and SERS properties of flower-like and hierarchical silver microcrystals

    Science.gov (United States)

    Yang, Jianhui; Cao, Beibei; Li, Huiqin; Liu, Bin

    2014-10-01

    Noble metal nano/microstructures have attracted considerable attention because of their unique properties and their various applications. Controlling the shape of noble metal nano/microstructures is a promising strategy to tailor their physical and chemical properties for various applications in fields such as biological labeling and imaging, catalysis, and sensing. Among various specific structures, flower-like and hierarchical silver nano/microstructures have attracted increasing interest because exploration of these novel nano/microstructures with unusual optical properties can provide new perspectives into the rational design of novel materials. It is significantly more challenging to develop facile and effective solution approaches for systematic manipulation of the shape of Ag nano/microstructures. In this article, we revisited the ascorbic acid reduction method to prepare flower-like silver microcrystal with plate petals and hierarchical Ag microcrystal on a large scale and in high purity. Ascorbic acid plays two roles of a reducing agent and a crystal growth regulator. Therefore, the molar ratio of ascorbic acid and silver nitrate is critical to the formation of Ag microcrystal. The controlling of the two different Ag microstructures can be achieved by adjusting the molar ratio of the reactants in aqueous medium at room temperature. The as-prepared Ag microcrystals were characterized by transmission electron microscopy, scanning electron microscopy, and X-ray diffraction. The flower-like Ag microcrystal with plate petals and hierarchical Ag microcrystal with nanoscale sharp tips and gaps could exhibit high catalytic activity and strong surface-enhanced Raman spectroscopy (SERS) activity due to the high surface area and the local electromagnetic field intensity enhancement, respectively. The potential application of the as-prepared Ag microcrystals in catalysis and SERS was investigated, which revealed that these two kinds of Ag microcrystals exhibit high

  5. Silver Nanoparticle-Embedded Thin Silica-Coated Graphene Oxide as an SERS Substrate

    Directory of Open Access Journals (Sweden)

    Xuan-Hung Pham

    2016-09-01

    Full Text Available A hybrid of Ag nanoparticle (NP-embedded thin silica-coated graphene oxide (GO@SiO2@Ag NPs was prepared as a surface-enhanced Raman scattering (SERS substrate. A 6 nm layer of silica was successfully coated on the surface of GO by the physical adsorption of sodium silicate, followed by the hydrolysis of 3-mercaptopropyl trimethoxysilane. Ag NPs were introduced onto the thin silica-coated graphene oxide by the reduction of Ag+ to prepare GO@SiO2@Ag NPs. The GO@SiO2@Ag NPs exhibited a 1.8-fold enhanced Raman signal compared to GO without a silica coating. The GO@SiO2@Ag NPs showed a detection limit of 4-mercaptobenzoic acid (4-MBA at 0.74 μM.

  6. Controllable fabrication of large-scale hierarchical silver nanostructures for long-term stable and ultrasensitive SERS substrates

    Science.gov (United States)

    Wu, Jing; Fang, Jinghuai; Cheng, Mingfei; Gong, Xiao

    2016-09-01

    In this work, we aim to prepare effective and long-term stable hierarchical silver nanostructures serving as surface-enhanced Raman scattering (SERS) substrates simply via displacement reaction on Aluminum foils. In our experiments, Hexadecyltrimethylammonium bromide (CTAB) is used as cationic surfactant to control the velocity of displacement reaction as well as the hierarchical morphology of the resultant. We find that the volume ratio of CTAB to AgNO3 plays a dominant role in regulating the hierarchical structures besides the influence of displacement reaction time. These as-prepared hierarchical morphologies demonstrate excellent SERS sensitivity, structural stability and reproducibility with low values of relative standard deviation less than 20 %. The high SERS analytical enhancement factor of ~6.7 × 108 is achieved even at the concentration of Crystal Violet (CV) as low as 10-7 M, which is sufficient for single-molecule detection. The detection limit of CV is 10-9 M in this study. We believe that this simple and rapid approach integrating advantages of low-cost production and high reproducibility would be a promising way to facilitate routine SERS detection and will get wide applications in chemical synthesis.

  7. Sers-Active Silver Nanoparticles in Ion-Exchanged Glass

    Science.gov (United States)

    Chen, Ya; Jaakola, Janne; Säynätjoki, Antti; Tervonen, Ari; Honkanen, Seppo

    We study synthesis and SERS activity of glass-embedded ion-exchanged silver nanoparticles formed by two different methods. Silver-sodium ion-exchange process with heat treatment was utilized on commercial microscope slides, while masked ion-exchange technique was performed on glass Corning 0211. The distribution of particles was studied by absorption spectroscopy and atomic force microscopy. After etching the glass surface to expose the particles, SERS performance of these particles was investigated with micro-Raman spectroscopy using Rhodamine 6G as the analyte.

  8. Determination of acetylsalicylic acid in commercial tablets by SERS using silver nanoparticle-coated filter paper

    Science.gov (United States)

    Sallum, Loriz Francisco; Soares, Frederico Luis Felipe; Ardila, Jorge Armando; Carneiro, Renato Lajarim

    2014-12-01

    In this work, filter paper was used as a low cost substrate for silver nanoparticles in order to perform the detection and quantification of acetylsalicylic acid by SERS in a commercial tablet. The reaction conditions were 150 mM of ammonium hydroxide, 50 mM of silver nitrate, 500 mM of glucose, 12 min of the reaction time, 45 °C temperature, pretreatment with ammonium hydroxide and quantitative filter paper (1-2 μm). The average size of silver nanoparticles deposited on the paper substrate was 180 nm. Adsorption time of acetylsalicylic acid on the surface of the silver-coated filter paper was studied and an adsorption time of 80 min was used to build the analytical curve. It was possible to obtain a calibration curve with good precision with a coefficient of determination of 0.933. The method proposed in this work was capable to quantify acetylsalicylic acid in commercial tablets, at low concentration levels, with relative error of 2.06% compared to the HPLC. The preparation of filter paper coated with silver nanoparticles using Tollen's reagent presents several advantages such as low cost of synthesis, support and reagents; minimum amount of residuals, which are easily treated, despite the SERS spectroscopy presenting fast analysis, with low sample preparation and low amount of reactants as in HPLC analysis.

  9. Annual report to Congress 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-07-01

    Section 205 of the Department of Energy Organization Act of 1977 established the Energy Information Administration (EIA). One of the mandates in this legislation is that EIA prepare for Congress an annual report summarizing both activities and information collected and published. EIA`s major 1997 accomplishments are profiled in the body of this edition of the Annual Report to Congress. Appendix A contains abstracts of significant reports issued by EIA in 1997, and a chart of all titles and a list of all feature articles published during the year. Appendix B contains graphs of selected performance measures. Appendix C lists contact information for EIA subject matter specialists. Appendix D lists the major laws which form the basis of EIA`s legislative mandate.

  10. Annual report to Congress, 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-04-01

    Section 205 of the Department of Energy Organization Act of 1977 established the Energy Information Administration (EIA). One of the mandates in this legislation is that EIA prepare for Congress an annual report summarizing both activities and information collected and published. EIA`s major 1998 accomplishments are profiled in the body of this edition of the Annual Report to Congress. Appendix A contains abstracts of significant reports issued by EIA in 1998 and a chart of all titles and a list of all feature articles published during the year. Appendix B contains graphs of selected performance measures. Appendix C lists contact information for EIA subject matter specialists. Appendix D lists the major laws which form the basis of EIA`s legislative mandate.

  11. Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates endocytosis and gap junction assembly in pancreatic cancer cells

    Science.gov (United States)

    Johnson, Kristen E.; Mitra, Shalini; Katoch, Parul; Kelsey, Linda S.; Johnson, Keith R.; Mehta, Parmender P.

    2013-01-01

    The molecular mechanisms regulating the assembly of connexins (Cxs) into gap junctions are poorly understood. Using human pancreatic tumor cell lines BxPC3 and Capan-1, which express Cx26 and Cx43, we show that, upon arrival at the cell surface, the assembly of Cx43 is impaired. Connexin43 fails to assemble, because it is internalized by clathrin-mediated endocytosis. Assembly is restored upon expressing a sorting-motif mutant of Cx43, which does not interact with the AP2 complex, and by expressing mutants that cannot be phosphorylated on Ser-279 and Ser-282. The mutants restore assembly by preventing clathrin-mediated endocytosis of Cx43. Our results also document that the sorting-motif mutant is assembled into gap junctions in cells in which the expression of endogenous Cx43 has been knocked down. Remarkably, Cx43 mutants that cannot be phosphorylated on Ser-279 or Ser-282 are assembled into gap junctions only when connexons are composed of Cx43 forms that can be phosphorylated on these serines and forms in which phosphorylation on these serines is abolished. Based on the subcellular fate of Cx43 in single and contacting cells, our results document that the endocytic itinerary of Cx43 is altered upon cell–cell contact, which causes Cx43 to traffic by EEA1-negative endosomes en route to lysosomes. Our results further show that gap-junctional plaques formed of a sorting motif–deficient mutant of Cx43, which is unable to be internalized by the clathrin-mediated pathway, are predominantly endocytosed in the form of annular junctions. Thus the differential phosphorylation of Cx43 on Ser-279 and Ser-282 is fine-tuned to control Cx43’s endocytosis and assembly into gap junctions. PMID:23363606

  12. Environmentally Friendly Preparation of Gold and Silver Nanoparticles for Sers Applications Using Biopolymer Pectin

    Science.gov (United States)

    Balachandran, Y. L.; Panarin, A. Y.; Khodasevich, I. A.; Terekhov, S. N.; Gutleb, A. C.; Girijaa, S.

    2015-01-01

    A facile, one-step, and environmentally friendly fabrication of anisotropic gold nanostructures and size-controlled spherical silver nanoparticles (NP) using biopolymer pectin is reported. The reduction of Au and Ag ions was carried out at room temperature using an increasing concentration of pectin, which acts as the single source of reducing and stabilizing agent. The as-formed NPs were studied by UV-vis, infrared Fourier transform and surface-enhanced Raman spectroscopies, as well as transmission electron microscopy and energy dispersive X-ray spectroscopy. A high yield of anisotropic gold nanostructures was observed at low concentrations of pectin, while its increase results in the formation of smaller sharp edged perfect triangles with a considerable number of quasi-spherically shaped gold NP. On the other hand, the size of spherical silver NP decreased as the biopolymer concentration in the solution increased. The surface-enhanced Raman scattering enhancement of different NPs was evaluated using a Cu-complex of cationic tetrakis(4-N-methylpyridyl)porphyrin as a probe molecule at 441.6 and 532 nm excitation. Great enhancement of Raman signal was obtained with a pectin-silver NP and for most of them their levels were higher than that for the routinely synthesized citrate silver NP.

  13. SERS internship fall 1995 abstracts and research papers

    Energy Technology Data Exchange (ETDEWEB)

    Davis, Beverly

    1996-05-01

    This report is a compilation of twenty abstracts and their corresponding full papers of research projects done under the US Department of Energy Science and Engineering Research Semester (SERS) program. Papers cover a broad range of topics, for example, environmental transport, supercomputers, databases, biology. Selected papers were indexed separately for inclusion the the Energy Science and Technology Database.

  14. Analysis and differentiation of seminal plasma via polarized SERS spectroscopy

    Directory of Open Access Journals (Sweden)

    Chen X

    2012-12-01

    Full Text Available Xiwen Chen,1,* Zufang Huang,1,* Shangyuan Feng,1 Jinhua Chen,2 Lan Wang,1 Peng Lu,1 Haishan Zeng,3 Rong Chen1 1Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, China; 2Fujian Provincial Hospital, Fuzhou, China; 3Imaging Unit – Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada*These authors contributed equally to this workAbstract: Polarized surface-enhanced Raman scattering (SERS spectroscopy was applied for obtaining biochemical information about the seminal plasma. The effect of different laser polarizations (nonpolarized, linear-polarized, right-handed circularly polarized, and left-handed circularly polarized on seminal plasma SERS spectroscopy was explored for the first time. The diagnostic performance in differentiating abnormal seminal plasma (n = 37 from normal seminal plasma (n = 24 was evaluated. A combination of principal component analysis (PCA and linear discriminant analysis (LDA was employed to develop diagnostic algorithms. Classification results of different laser polarizations demonstrated different diagnostic sensitivities and specificities, among which, left-handed circularly polarized laser excitation showed the best diagnostic result (95.8% sensitivity and 64.9% specificity. Our exploratory study demonstrated that SERS spectroscopy with left-handed circularly polarized laser excitation has the potential for becoming a new diagnostic method in semen-quality assessment.Keywords: SERS, seminal plasma, PCA-LDA, polarized laser excitation, silver nanoparticles, biomolecule chirality

  15. Plasmonics-based SERS nanobiosensor for homogeneous nucleic acid detection.

    Science.gov (United States)

    Wang, Hsin-Neng; Fales, Andrew M; Vo-Dinh, Tuan

    2015-05-01

    Developing a simple and efficient nucleic acid detection technology is essential for clinical diagnostics. Here, we describe a new conceptually simple and selective "turn on" plasmonics-based nanobiosensor, which integrates non-enzymatic DNA strand-displacement hybridization for specific nucleic acid target identification with surface-enhanced Raman scattering (SERS) detection. This SERS nanobiosensor is a target label-free, and rapid nanoparticle-based biosensing system using a homogeneous assay format that offers a simple and efficient tool for nucleic acid diagnostics. Our results showed that the nanobiosensor provided a limit of detection of ~0.1nM (200amol) in the current bioassay system, and exhibited high specificity for single nucleotide mismatch discrimination. Surface-enhanced Raman scattering (SERS) is a sensitive technique that enhances Raman scattering by molecules adsorbed on rough metal surfaces. The enhancement means that the technique may even detect single molecules. In this article, the authors describe a simple and efficient nucleic acid detection technology using SERS, with "OFF-to-ON" signal switch upon nucleic acid target identification and capture, which provides high sensitivity and specificity for single nucleotide mismatch discrimination. This new technology will be most welcomed in clinical diagnostics. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. A Novel Substrate Used for Simultaneous SERS and RAIR

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A novel substrate, whose silver particles were deposited on both sides of a slide, was made by silver mirror reaction. Its two sides had different thinness and reflectivity and they were suited for the test of surface enhanced Raman spectroscopy (SERS) and reflection absorption infrared spectroscopy (RAIR) technique respectively.

  17. 2014 HPC Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Jennings, Barbara [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2014-10-01

    Our commitment is to support you through delivery of an IT environment that provides mission value by transforming the way you use, protect, and access information. We approach this through technical innovation, risk management, and relationships with our workforce, Laboratories leadership, and policy makers nationwide. This second edition of our HPC Annual Report continues our commitment to communicate the details and impact of Sandia’s large-scale computing resources that support the programs associated with our diverse mission areas. A key tenet to our approach is to work with our mission partners to understand and anticipate their requirements and formulate an investment strategy that is aligned with those Laboratories priorities. In doing this, our investments include not only expanding the resources available for scientific computing and modeling and simulation, but also acquiring large-scale systems for data analytics, cloud computing, and Emulytics. We are also investigating new computer architectures in our advanced systems test bed to guide future platform designs and prepare for changes in our code development models. Our initial investments in large-scale institutional platforms that are optimized for Informatics and Emulytics work are serving a diverse customer base. We anticipate continued growth and expansion of these resources in the coming years as the use of these analytic techniques expands across our mission space. If your program could benefit from an investment in innovative systems, please work through your Program Management Unit ’s Mission Computing Council representatives to engage our teams.

  18. NAGRA annual report 2012

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2013-07-01

    This report issued by the Swiss National Co-operative for the Disposal of Radioactive Waste NAGRA reviews the co-operative’s activities in the year 2012. In January 2012, the Swiss Federal Office of Energy (SFOE) announced the 20 proposals made by NAGRA for siting areas for nuclear waste repository surface facilities. The discussions within the framework of regional participation focused on the location and layout of the surface facility within the defined planning perimeter. Progress in NAGRA’s work on the safety-based comparison and further narrowing-down of the geological siting regions for the repositories is reported on. NAGRA’s mandate includes its responsibility for preparing the technical and scientific basis for the safe, long-term management of nuclear waste. Progress on the so-called ‘Sectorial Plan’ which addresses the fundamental question of how to safely dispose of all types of radioactive waste is reported on, as is the process of defining locations in Switzerland for one or more deep repositories for nuclear wastes. Public participation in the process is reported on and geochemical considerations involved in designing the repositories are looked at. The work done in the rock laboratories is summarised and international co-operation is noted. The NAGRA ‘Time Ride’ exhibition is also briefly looked at. The report is completed with listings of those involved in NAGRA’s Board of Directors, commissions and auditors along with the annual financial statement for 2012.

  19. LLNL NESHAPs 1995 annual report

    Energy Technology Data Exchange (ETDEWEB)

    Gallegos, G.M.; Harrach, R.J.; Biermann, A.H.; Tate, P.J. [and others

    1996-06-01

    This annual report is prepared pursuant to the National Emissions Standards for Hazardous Air Pollutants (NESHAPs) 40 CFR Part 61, Subpart H; Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an effective dose equivalent (EDE) of 10 mrem to any member of the public. This document contains the EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from 1995 operations.

  20. 2011 ANNUAL SITE ENVIRONMENTAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, A.; Eddy, T.; Jannik, T.; Terry, B.; Cauthen, K.; Coward, L.; Dunaway-Ackerman, J.; Wilson, M.; Hutchison, J.; O' Quinn, S.

    2012-10-01

    The Savannah River Site Environmental Report for 2011 (SRNS-STI-2012-00200) is prepared for the U.S. Department of Energy (DOE) according to requirements of DOE Order 231.1 B, “Environment, Safety and Health Reporting." The annual SRS Environmental Report has been produced for more than 50 years. Several hundred copies are and interested individuals. The report’s purpose is to: present summary environmental data that characterize site environmental management performance; describe compliance status with respect to environmental standards and requirements; highlight significant programs and efforts.

  1. Geothermal Energy Development annual report 1979

    Energy Technology Data Exchange (ETDEWEB)

    1980-08-01

    This report is an exerpt from Earth Sciences Division Annual Report 1979 (LBL-10686). Progress in thirty-four research projects is reported including the following area: geothermal exploration technology, geothermal energy conversion technology, reservoir engineering, and geothermal environmental research. Separate entries were prepared for each project. (MHR)

  2. CSWE Annual Report 2014-2015

    Science.gov (United States)

    Council on Social Work Education, 2015

    2015-01-01

    Each "CSWE Annual Report" covers highlights of the organization's activities for a complete fiscal year (which runs from July 1 of one year to June 30 of the next). Prepared by a staff team and published through the Council on Social Work Education (CSWE) Office on Member and Communication Services, the report serves to inform members…

  3. Earth Sciences Division annual report 1981. [Lead abstract

    Energy Technology Data Exchange (ETDEWEB)

    1982-09-01

    Separate abstracts were prepared for the 59 papers of the 1981 annual report of the Earth Sciences Division at Lawrence Berkeley Laboratory. The general topics covered included nuclear waste isolation, geophysics and reservoir engineering, and geosciences. (KRM)

  4. Idaho National Laboratory PCB Annual Document Log and Annual Records Report for Calendar Year 2013

    Energy Technology Data Exchange (ETDEWEB)

    no name on report

    2014-06-01

    The requirements for the reporting of polychlorinated biphenyl (PCB)-related activities are found in 40 Code of Federal Regulations (CFR) 761 Subpart J, "General Records and Reports." The PCB Annual Document Log is a detailed record of the PCB waste handling activities at the facility. The facility must prepare it each year by July 1 and maintain it at the facility for at least 3 years after the facility ceases using or storing PCBs and PCB items. While submittal of the PCB Annual Document Log to the U.S. Environmental Protection Agency (EPA) is not required by regulation, EPA has verbally requested in telephone conversations that this report be submitted to them on an annual basis. The Annual Document Log section of this report meets the requirements of 40 CFR 761.180(a)(2), as applicable, while the Annual Records section meets the requirement of 40 CFR 761.180(a)(1).

  5. Black silicon SERS substrate: effect of surface morphology on SERS detection and application of single algal cell analysis.

    Science.gov (United States)

    Deng, Yu-Luen; Juang, Yi-Je

    2014-03-15

    In this study, we have investigated the effect of the surface morphology of the black silicon substrate on surface enhanced Raman spectroscopy (SERS) and explored its application of single algal cell detection. By adjusting the O2 and SF6 flow rates in the cryogenic plasma etching process, different surface morphologies of the black silicon substrate was produced without performing the lithographic process. It was found the Raman signals were better enhanced as the tip density of the black silicon substrate increased. In addition, as the thickness of the deposited gold layer increased, the SERS effect increased as well, which could be owing to the generation of more hot spots by bridging individual silicon tips through deposition of gold layer. For the black silicon substrate with tip density of 30 tips/μm(2) and covered by 400 nm deposited gold layer, the detection limit of 10 fM R6G solution concentration with uniform SERS effect across the substrate was achieved. Furthermore, detection of individual algal cell (Chlorella vulgaris) was performed at the SERS substrate as fabricated and the Raman signals of carotenoid and lipid were substantially enhanced.

  6. Processing ser and estar to locate objects and events

    Science.gov (United States)

    Dussias, Paola E.; Contemori, Carla; Román, Patricia

    2016-01-01

    In Spanish locative constructions, a different form of the copula is selected in relation to the semantic properties of the grammatical subject: sentences that locate objects require estar while those that locate events require ser (both translated in English as ‘to be’). In an ERP study, we examined whether second language (L2) speakers of Spanish are sensitive to the selectional restrictions that the different types of subjects impose on the choice of the two copulas. Twenty-four native speakers of Spanish and two groups of L2 Spanish speakers (24 beginners and 18 advanced speakers) were recruited to investigate the processing of ‘object/event + estar/ser’ permutations. Participants provided grammaticality judgments on correct (object + estar; event + ser) and incorrect (object + ser; event + estar) sentences while their brain activity was recorded. In line with previous studies (Leone-Fernández, Molinaro, Carreiras, & Barber, 2012; Sera, Gathje, & Pintado, 1999), the results of the grammaticality judgment for the native speakers showed that participants correctly accepted object + estar and event + ser constructions. In addition, while ‘object + ser’ constructions were considered grossly ungrammatical, ‘event + estar’ combinations were perceived as unacceptable to a lesser degree. For these same participants, ERP recording time-locked to the onset of the critical word ‘en’ showed a larger P600 for the ser predicates when the subject was an object than when it was an event (*La silla es en la cocina vs. La fiesta es en la cocina). This P600 effect is consistent with syntactic repair of the defining predicate when it does not fit with the adequate semantic properties of the subject. For estar predicates (La silla está en la cocina vs. *La fiesta está en la cocina), the findings showed a central-frontal negativity between 500–700 ms. Grammaticality judgment data for the L2 speakers of Spanish showed that beginners were significantly less

  7. Leucipo, Demócrito e Kant: uma Reflexão sobre a Equivalência entre Ser e Não-Ser

    OpenAIRE

    Eberth Eleuterio dos Santos

    2015-01-01

    De início, apresentaremos a tese de Demócrito e Leucipo, segundo a qual o ser não é mais que o não-ser, tendo como contraponto o pensamento eleata acerca da inexistência necessária do não-ser. Esta discussão nos remete à oposição entre o pleno (cheio) e o vazio que será posteriormente traduzida na oposição entre o ser e o nada (ou o não-ser). Desse modo, a oposição entre o pleno e o vazio é uma oposição que se desloca para o ser e o não-ser. Em seguida, faremos a apreciação do escrito pré-crí...

  8. The Oak Ridge Reservation Annual Site Environmental Report Summary, 2007

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2009-02-28

    The Oak Ridge Reservation Annual Site Environmental Report is prepared and published each year to inform the public of the environmental activities that take place on the reservation and in the surrounding areas. It is written to comply with DOE Order 231.1A, Environment, Safety, and Health Reporting. This document has been prepared to present the highlights of the Oak Ridge Reservation Annual Site Environmental Report 2007 in an easy-to-read, summary format.

  9. Franz Brentano: equivocidad del ser y objeto intencional

    Directory of Open Access Journals (Sweden)

    Mario Ariel González Porta

    2002-06-01

    Full Text Available Este trabajo se propone probar que el concepto brentaniano de intencionalidad, más propiamente, su teoría de la inmanencia del objeto intencional y el posterior abandono de la misma, no pueden ser comprendidos de modo adecuado sino en el marco de las preocupaciones ontológicas del autor y, en particular, de su defensa inicial y subsiguiente rechazo de la tesis de la equivocidad del ser.This work aims to prove that the brentanian notion of intentionality, and particularly his conceptions of intentional object and immanence, cannot be properly understood but in the boundaries of the author' s ontological concerns, particularly his former defense and later denial of the equivocity of being.

  10. Synthesis, characterization and SERS activity of biosynthesized silver nanoparticles

    Science.gov (United States)

    Bindhu, M. R.; Sathe, V.; Umadevi, M.

    2013-11-01

    Silver nanoparticles were rapidly synthesized using Moringa oleifera flower extract as the reducing agent shows surface plasmon resonance peak at 439 nm. The size and shape of the nanoparticles controlled by varying the concentration of M. oleifera flower extract in the reaction medium. The synthesized silver nanoparticles were well-dispersed spherical nanoparticles with the average size of 14 nm. The retinoic acid present in M. oleifera flower extract used as reducing agent and proteins was responsible for capping of the bioreduced silver nanoparticles. The obtained nanoparticle shows size-dependent SERS activity. The SERS spectrum indicates that the pyridine adsorbed on the silver surface in a stand-on orientation via its nitrogen lone pair electrons.

  11. Highly reproducible SERS arrays directly written by inkjet printing

    Science.gov (United States)

    Yang, Qiang; Deng, Mengmeng; Li, Huizeng; Li, Mingzhu; Zhang, Cong; Shen, Weizhi; Li, Yanan; Guo, Dan; Song, Yanlin

    2014-12-01

    SERS arrays with uniform gold nanoparticle distribution were fabricated by direct-writing with an inkjet printing method. Quantitative analysis based on Raman detection was achieved with a small standard statistical deviation of less than 4% for the reproducibility and less than 5% for the long-term stability for 12 weeks.SERS arrays with uniform gold nanoparticle distribution were fabricated by direct-writing with an inkjet printing method. Quantitative analysis based on Raman detection was achieved with a small standard statistical deviation of less than 4% for the reproducibility and less than 5% for the long-term stability for 12 weeks. Electronic supplementary information (ESI) available: Additional information on the experimental details, gold nanoparticle characterization, and theoretical calculation for the diameters of contact area of droplets on substrates with different contact angles. See DOI: 10.1039/c4nr04656k

  12. Simultaneous SERS detection of copper and cobalt at ultratrace levels.

    Science.gov (United States)

    Tsoutsi, Dionysia; Guerrini, Luca; Hermida-Ramon, Jose Manuel; Giannini, Vincenzo; Liz-Marzán, Luis M; Wei, Alexander; Alvarez-Puebla, Ramon A

    2013-07-07

    We report a SERS-based method for the simultaneous and independent determination of two environmental metallic pollutants, Cu(ii) and Co(ii). This was achieved by exploiting the coordination-sensitive Raman bands of a terpyridine (TPY) derivative for detecting transition metal ions. Changes in the vibrational SERS spectra of dithiocarbamate anchored terpyridine (TPY-DTC) were correlated as a function of each metal ion concentration, with limits of detection comparable to those of several conventional analytical methods. Simultaneous detection of ultratrace levels of Co(ii) in the presence of high Cu(ii) concentration was also demonstrated, supporting the potential of this sensing strategy for monitoring potable water supplies.

  13. Hierarchical Ag mesostructures for single particle SERS substrate

    Science.gov (United States)

    Xu, Minwei; Zhang, Yin

    2017-01-01

    Hierarchical Ag mesostructures with highly rough surface morphology have been synthesized at room temperature through a simple seed-mediated approach. Electron microscopy characterizations indicate that the obtained Ag mesostructures exhibit a textured surface morphology with the flower-like architecture. Moreover, the particle size can be tailored easily in the range of 250-500 nm. For the growth process of the hierarchical Ag mesostructures, it is believed that the self-assembly mechanism is more reasonable rather than the epitaxial overgrowth of Ag seed. The oriented attachment of nanoparticles is revealed during the formation of Ag mesostructures. Single particle surface enhanced Raman spectra (sp-SERS) of crystal violet adsorbed on the hierarchical Ag mesostructures were measured. Results reveal that the hierarchical Ag mesostructures can be highly sensitive sp-SERS substrates with good reproducibility. The average enhancement factors for individual Ag mesostructures are estimated to be about 106.

  14. Aplicaciones del láser en odontologia

    OpenAIRE

    España Tost, A. J. (Antonio Jesús); Arnabat Domínguez, Josep; Berini Aytés, Leonardo; Gay Escoda, Cosme

    2004-01-01

    Existen diferentes tipos de láseres que pueden tener aplicación en Odontología, y éstos pueden ser utilizados con diferentes fines. El propósito de este artículo es comentar, de forma genérica, el empleo de diferentes tipos de láseres en diversos tipos de tratamientos habituales en la práctica odontológica. No todos los láseres producen los mismos efectos; tampoco un mismo láser produce el mismo efecto sobre diferentes tejidos, y, según sean los parámetros de emisión utilizados, incluso el mi...

  15. A Novel Uncoded SER/BER Estimation Method

    Directory of Open Access Journals (Sweden)

    Mahesh Patel

    2015-06-01

    Full Text Available Due to the rapidly increasing data speed requirement, it has become essential to smartly utilize the available frequency spectrum. In wireless communications systems, channel quality parameters are often used to enable resource allocation techniques that improve system capacity and user quality. The uncoded bit or symbol error rate (SER is specified as an important parameter in the second and third generation partnership project (3GPP. Nonetheless, techniques to estimate the uncoded SER are usually not much published. This paper introduces a novel uncoded bit error rate (BER estimation method using the accurate-bits sequence of the new channel codes over the AWGN channel. Here, we have used the new channel codes as a forward error correction coding scheme for our communication system. This paper also presents the simulation results to demonstrate and compare the estimation accuracy of the proposed method over the AWGN channel.

  16. Reproducible Ultrahigh Electromagnetic SERS Enhancement in Nanosphere-Plane Junctions

    CERN Document Server

    Long, Jing; Li, Hongquan; Yang, Tian

    2015-01-01

    Surface enhanced Raman scattering (SERS) in nanoscale hotspots has been placed great hopes upon for identification of minimum chemical traces and in-situ investigation of single molecule structures and dynamics. However, previous work consists of either irreproducible enhancement factors (EF) from random aggregates, or moderate EFs despite better reproducibility. Consequently, systematic study of SERS at the single and few molecules level is still very limited, and the promised applications are far from being realized. Here we report EFs as high as the most intense hotspots in previous work yet achieved in a reproducible and well controlled manner, that is, electromagnetic EFs (EMEF) of 10^9~10 with an error down to 10^+/-0.08 from gold nanospheres on atomically flat gold planes under radially polarized (RP) laser excitation. In addition, our experiment reveals the EF's unexpected nonlinearity under as low as hundreds of nanowatts of laser power.

  17. Ser649 and Ser650 are the major determinants of protein kinase A-mediated activation of human hormone-sensitive lipase against lipid substrates

    DEFF Research Database (Denmark)

    Krintel, Christian; Osmark, Peter; Larsen, Martin Rask;

    2008-01-01

    Hormone-sensitive lipase (HSL) is a key enzyme in the mobilization of fatty acids from stored triacylglycerols. Its activity is regulated by reversible protein phosphorylation. In rat HSL Ser563, Ser659 and Ser660 have been shown to be phosphorylated by protein kinase A (PKA) in vitro as well...

  18. Donació de llibre "El arte de ser totonaca"

    OpenAIRE

    Solanilla Demestre, Victòria

    2009-01-01

    El 5 de febrer de 2009 a la Biblioteca d'Humanitats, en un acte senzill i alhora carregat de significació, una representació de l'Estat de Veracruz va fer una donació del llibre "El arte de ser totonoca" al Grup d'Estudis Precolombins de la UAB, que al seu torn en va fer donació a la Biblioteca d'Humanitats

  19. Sobre mi traducción de "Ser y Tiempo"

    OpenAIRE

    2005-01-01

    Este escrito pretende dos cosas: primero, desarrollar brevemente mi propia idea de lo que es traducir, y, segundo, explicar la solución que di a ciertos problemas que surgieron en mi traducción de la obra "Ser y Tiempo" de Heidegger. (In this article the author develops his own idea of a translation and explains the solutions given to problems arisen in the translation of Heidegger’s Being and Time.)

  20. Sobre mi traducción de "Ser y Tiempo"

    Directory of Open Access Journals (Sweden)

    Jorge Eduardo Rivera

    2005-12-01

    Full Text Available Este escrito pretende dos cosas: primero, desarrollar brevemente mi propia idea de lo que es traducir, y, segundo, explicar la solución que di a ciertos problemas que surgieron en mi traducción de la obra "Ser y Tiempo" de Heidegger. (In this article the author develops his own idea of a translation and explains the solutions given to problems arisen in the translation of Heidegger’s Being and Time.

  1. Plasmonic Paper as a Highly Efficient SERS Substrate

    Science.gov (United States)

    2012-09-01

    diagnostic devices by printing microfluidic patterns on paper, which create hydrophobic barriers that define channels and reaction zones to fabricate an...trace detection on real-world surfaces,” ACS Appl. Mater. Interfaces, 2(12), 3429-3435 (2010). [24] Yu, W. W. and White, I. M., “ Inkjet printed ...scaffolding format. We discuss the fabrication , physical characterization and SERS activity of our novel substrates using non-resonant analytes. 15

  2. Significado de ser docente para el adulto mayor sanfernandino

    OpenAIRE

    Martina, Martha; Facultad de Medicina, UNMSM; Santos, Gladys; Facultad de Medicina, UNMSM; Lema, Julia; Facultad de Medicina, UNMSM

    2013-01-01

    Objetivos: Comprender el significado de ser docente para el adulto mayor de la Facultad de Medicina de San Marcos y describir las motivaciones que determinan, de alguna forma, su decisión de postergar su retiro, no obstante haber cumplido con los requisitos legales para la jubilación. Diseño: Estudio de caso cualitativo. Institución: Facultad de Medicina, UNMSM. Participantes: Docentes adulto mayores de la Facultad de Medicina. Intervenciones: Entrevista a profundidad. Principales medidas de ...

  3. Long-term photopolarimetric variability of DD Ser

    Science.gov (United States)

    Belan, S. P.; Shakhovskoy, D. N.

    2013-12-01

    We present some results of UBVRI-photopolarimetric observations of DD Ser, a poorly studied and understood Ae-star. The long-term component of light variation is detected, with the period of about fifteen years. Variability of the degree and positional angle of linear polarization is observed as well. Analysis of correlation between photometric and polarimetric data shows that observed light minima are likely caused by variability of circumstellar extinction produced by dust.

  4. GEOGRAFIA E ONTOLOGIA: O FUNDAMENTO GEOGRÁFICO DO SER

    Directory of Open Access Journals (Sweden)

    Elvio Rodrigues Martins

    2007-08-01

    Full Text Available Este trabalho busca a definição do fundamento geográfico como uma das determinações constituintes do ser do homem e da sociedade. Para tanto, retoma alguns dos fundamentos epistemológicos da ciência geográfica, no sentido de redefini-los, e com isso estabelecer novas bases para a relação entre ontologia e Geografia.

  5. Laser in medicine Láser en medicina

    Directory of Open Access Journals (Sweden)

    Juan C. Cárdenas

    1993-03-01

    Full Text Available The fundamentals of laser functioning and a brief historic description on the subject are presented; laser Is classified according to emission potency, materials with which it is built and pulsation. Different mechanisms of action of laser as well as Its indications and contraindications are discussed. Emphasis is given to low-power laser. Local and foreign experiences with Its medlcal use are briefly described

    Se revisan los principios fundamentales del funcionamiento del láser, se hace una breve revisión histórica del mismo y se lo clasifica de acuerdo con la potencia de emisión, los materiales de fabricación y la pulsación. Se enumeran los diferentes mecanismos de acción del láser y sus indicaciones y contraindicaciones haciendo énfasis en lo concerniente al láser de baja potencia, tanto pulsado como no pulsado; se alude a las experiencias nacionales y de otros países.

  6. A possible giant planet orbiting the cataclysmic variable LX Ser

    CERN Document Server

    Li, K; Zhou, J -L; Wu, D -H; Guo, D -F; Jiang, Y -G; Gao, D -Y; Chen, X; Wang, X -Y

    2016-01-01

    LX Ser is a deeply eclipsing cataclysmic variable with an orbital period of $0.^d 1584325$. Sixty two new eclipse times were determined by our observations and the AAVSO International Data base. Combining all available eclipse times, we analyzed the O-C behavior of LX Ser. We found that the O-C diagram of LX Ser shows a sinusoidal oscillation with a period of 22.8 yr and an amplitude of 0.00035 days. Two mechanisms (i.e., the Applegate mechanism and the light travel time effect) are applied to explain the cyclic modulation. We found that the Applegate mechanism is difficult to explain the cyclic oscillation in the orbital period. Therefore, the cyclic period change is most likely to be caused by the light travel time effect due to the presence of a third body. The mass of the tertiary component was determined to be $M_3\\sim7.5 M_{Jup}$. We supposed that the tertiary companion is plausible a giant planet. The stability of the giant planet was checked, and we found that the multiple system is stable.

  7. A possible giant planet orbiting the cataclysmic variable LX Ser

    Science.gov (United States)

    Li, Kai; Hu, Shaoming; Zhou, Jilin; Wu, Donghong; Guo, Difu; Jiang, Yunguo; Gao, Dongyang; Chen, Xu; Wang, Xianyu

    2017-02-01

    LX Ser is a deeply eclipsing cataclysmic variable with an orbital period of 0.1584325 d. 62 new eclipse times were determined by our observations and the AAVSO International Data base. Combining all available eclipse times, we analyzed the O - C behavior of LX Ser. We found that the O - C diagram of LX Ser shows a sinusoidal oscillation with a period of 22.8 yr and an amplitude of 0.00035 d. Two mechanisms (i.e., the Applegate mechanism and the light-travel time effect) are applied to explain the cyclic modulation. We found that it is difficult to apply the Applegate mechanism to explain the cyclic oscillation in the orbital period. Therefore, the cyclic period change is most likely to be caused by the light-travel time effect due to the presence of a third body. The mass of the tertiary component was determined to be M3 ∼ 7.5 MJup. We supposed that the tertiary companion is plausibly a giant planet. The stability of the giant planet was checked, and we found that the multiple system is stable.

  8. Interpenetrating polyaniline-gold electrodes for SERS and electrochemical measurements

    Science.gov (United States)

    West, R. M.; Semancik, S.

    2016-11-01

    Facile fabrication of nanostructured electrode arrays is critical for development of bimodal SERS and electrochemical biosensors. In this paper, the variation of applied potential at a polyaniline-coated Pt electrode is used to selectivity deposit Au on the polyaniline amine sites or on the underlying Pt electrode. By alternating the applied potential, the Au is grown simultaneously from the top and the bottom of the polyaniline film, leading to an interpenetrated, nanostructured polymer-metal composite extending from the Pt electrode to the electrolyte solution. The resulting films have unique pH-dependent electrochemical properties, e.g. they retain electrochemical activity in both acidic and neutral solutions, and they also include SERS-active nanostructures. By varying the concentration of chloroaurate used during deposition, Au nanoparticles, nanodendrites, or nanosheets can be selectively grown. For the films deposited under optimal conditions, using 5 mmol/L chloroaurate, the SERS enhancement factor for Rhodamine 6G was found to be as high as 1.1 × 106 with spot-to-spot and electrode-to-electrode relative standard deviations as low as 8% and 12%, respectively. The advantages of the reported PANI-Au composite electrodes lie in their facile fabrication, enabling the targeted deposition of tunable nanostructures on sensing arrays, and their ability to produce orthogonal optical and electrochemical analytical results.

  9. SERS study of the complexes of thiamine derivatives with pyruvate

    Science.gov (United States)

    Strekal, N. D.; Gachko, G. A.; Kivach, L. N.; Maskevich, S. A.

    1992-03-01

    The SER spectra of thiamine (T) 4'-hydroxythiamine (HOT), thiamine disphoshate (TDP) on silver electrode at acidic and neutral solution have been investigated. The influence of pyruvate (Pyr) on SER spectra at various applied voltages 0 - -0, 65 V has been studied. In the acidic solution T and TDP interact with the surface by means of the heteroatom of N of the pyrimidine and heteroatoms of N and S of thiazolium ring. The characteristic bands at 665, 755, 1210 and 1640 cm -1 are observed in SERS spectra. It is not detected the interaction of N atom of thiazolium ring of HOT with the silver surface. The Cl - ions play an important role in adsorption of these molecules. In the acidic solution Pyr enhances the interaction of thiazolium moiety of TDP with surface and decrease that of HOT. In the neutral solution and applied voltages more positive than> -0,5 V molecules of T derivatives desorptes and Pyr promotes that. The possible mechanisms of the influence of Pyr on adsorption of the T derivatives are discussed.

  10. Label-free monitoring of individual DNA hybridization using SERS

    Science.gov (United States)

    Qi, Ji; Zeng, Jianbo; Zhao, Fusheng; Santos, Greggy M.; Lin, Steven Hsesheng; Raja, Balakrishnan; Strych, Ulrich; Willson, Richard C.; Shih, Wei-Chuan

    2015-03-01

    Sequence-specific detection of DNA hybridization at the single-molecule level has been instrumental and gradually become a ubiquitous tool in a wide variety of biological and biomedical applications such as clinical diagnostics, biosensors, and drug development. Label-free and amplification-free schemes are of particular interest because they could potentially provide in situ monitoring of individual hybridization events, which may lead to techniques for discriminating subtle variations due to single-base modification without stringency control or repetitive thermal cycling. Surface-enhanced Raman spectroscopy (SERS) has been widely used for molecular detection and identification by exploiting the localized surface plasmon resonance effect when the target molecules are near gold or silver nanostructures. However, effective and robust SERS assays have yet become a reality for trace detection. Recently, we have developed a SERS substrate by shaping nanoporous gold thin films into monolithic submicron disks, called nanoporous gold disks (NPGD). Here we demonstrate in situ monitoring of the same immobilized ssDNA molecules and their individual hybridization events.

  11. Ser649 and Ser650 are the major determinants of protein kinase A-mediated activation of human hormone-sensitive lipase against lipid substrates.

    Directory of Open Access Journals (Sweden)

    Christian Krintel

    Full Text Available BACKGROUND: Hormone-sensitive lipase (HSL is a key enzyme in the mobilization of fatty acids from stored triacylglycerols. Its activity is regulated by reversible protein phosphorylation. In rat HSL Ser563, Ser659 and Ser660 have been shown to be phosphorylated by protein kinase A (PKA in vitro as well as in vivo. METHODOLOGY/PRINCIPAL FINDINGS: In this study we employed site-directed mutagenesis, in vitro phosphorylation and mass spectrometry to show that in vitro phosphorylation of human HSL by PKA occurs primarily on Ser649 and Ser650 (Ser659 and Ser660 in rat HSL. The wild type enzyme and four mutants were expressed in C-terminally His-tagged form in Sf9 insect cells and purified to homogeneity. HSL variants in which Ser552 and/or Ser554 were mutated to Ala or Glu retained both lipolytic and non-lipolytic activity and were phosphorylated by PKA and activated to a similar extent as the wild type enzyme. (32P-labeling studies revealed that the bulk of the phosphorylation was on the Ser649/Ser650 site, with only a minor phosphorylation of Ser552 and Ser554. MS/MS analysis demonstrated that the peptide containing Ser649 and Ser650 was primarily phosphorylated on Ser650. The mutant lacking all four serines had severely reduced lipolytic activity, but a lesser reduction in non-lipolytic activity, had S(0.5 values for p-nitrophenol butyrate and triolein comparable to those of wild type HSL and was not phosphorylated by PKA. PKA phosphorylation of the wild type enzyme resulted in an increase in both the maximum turnover and S(0,5 using the TO substrate. CONCLUSIONS: Our results demonstrate that PKA activates human HSL against lipid substrates in vitro primarily through phosphorylation of Ser649 and Ser650. In addition the results suggest that Ser649 and Ser650 are located in the vicinity of a lipid binding region and that PKA phosphorylation controls the accessibility of this region.

  12. Hierarchical Self-Assembly of Cu7Te5 Nanorods into Superstructures with Enhanced SERS Performance.

    Science.gov (United States)

    Zheng, Jiaojiao; Dai, Baosong; Liu, Jia; Liu, Jialong; Ji, Muwei; Liu, Jiajia; Zhou, Yuanmin; Xu, Meng; Zhang, Jiatao

    2016-12-28

    This paper reports a strategy to get self-assembly of Cu7Te5 nanorods into hierarchical superstructures: the side-by-side self-assembly of nanorods into microscale one-dimensional (1D) nanowires (primary structure), the side-by-side alignments of the 1D nanowires into two-dimensional (2D) nanowire bundles (secondary structure), and the further rolling up of the 2D bundles into three-dimensional (3D) microtubes (tertiary structure). It was found that the oleylamine (OLA)/n-dodecanethiol (DDT) mixture as a binary capping agent was key to produce Cu7Te5 nanorods in the quantum size regime with high monodispersity, and this was a prerequisite for their hierarchical self-assembly based on elaborate control of the solvent evaporation process. The obtained Cu7Te5 microtube superstructures were used as SERS substrate and showed much stronger SERS enhancement than the as-prepared Cu7Te5 nanorods before assembly. This was probably ascribed to the remarkably enhanced local electromagnetic field arising from the plasmon coupling of Cu7Te5 nanorods in the well-assembled superstructures.

  13. One step 'dip' and 'use' Ag nanostructured thin films for ultrahigh sensitive SERS Detection.

    Science.gov (United States)

    Rajkumar, Kanakaraj; Jayram, Naidu Dhanpal; Mangalaraj, Devanesan; Rajendra Kumar, Ramasamy Thangavelu

    2016-11-01

    A simple one step galvanic displacement method which involves dipping of the silicon substrate in the AgNO3/HF solution and using it for SERS application without any further process is demonstrated. The size and shape of the Ag nanoparticles changes as the deposition time is increased. Initially the shape of the particles was nearly spherical and as it grows, becomes oblong and then coalesce to form a discontinuous film with vertically grown hierarchical Ag nanostructures. The sizes of the deposited particles were in the ranges from 30nm to a discontinuous film. It also demonstrated a highly sensitive chemical detection by surface-enhanced Raman scattering of rhodamine 6G dye, down to 10(-16)M concentration. Prepared samples were able to detect lower concentrations of Melamine. Discontinuous thin films with hierarchical Ag nanostructures were obtained for 5min Ag deposition. The formation of Hot spots between the discontinuous islands and also along the hierarchical structures is responsible for the high SERS enhancement. This simple one step, fast, non-lithographic and cost effective method can be applied for various label free detection of analytes of importance.

  14. Annual Statistical Supplement, 2008

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2008 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  15. Annual Statistical Supplement, 2004

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2004 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  16. Annual Statistical Supplement, 2006

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2006 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  17. Annual Statistical Supplement, 2016

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2016 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  18. Annual Statistical Supplement, 2010

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2010 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  19. Annual Statistical Supplement, 2002

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2002 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  20. Annual Statistical Supplement, 2003

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2003 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  1. Annual Statistical Supplement, 2011

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2011 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  2. Annual Statistical Supplement, 2000

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2000 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  3. Annual Statistical Supplement, 2015

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2015 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  4. Annual Statistical Supplement, 2009

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2009 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  5. Annual Statistical Supplement, 2014

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2014 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  6. Annual Statistical Supplement, 2007

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2007 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  7. Annual Statistical Supplement, 2005

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2005 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  8. Annual Statistical Supplement, 2001

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2001 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  9. Nanostructuring of thin Au films deposited on ordered Ti templates for applications in SERS

    Science.gov (United States)

    Grochowska, Katarzyna; Siuzdak, Katarzyna; Macewicz, Łukasz; Skiba, Franciszek; Szkoda, Mariusz; Karczewski, Jakub; Burczyk, Łukasz; Śliwiński, Gerard

    2017-10-01

    In this work the results on thermal nanostructuring of the Au films on Ti templates as well as morphology and optical properties of the obtained structures are reported. The bimetal nanostructures are fabricated in a multi-step process. First, the titania nanotubes are produced on the surface of Ti foil by anodization in an ethylene glycol-water solution containing fluoride ions. This is followed by chemical etching in oxalic acid and results in a highly ordered dimpled surface. Subsequently, thin gold films (5-20 nm) are deposited onto prepared Ti substrates by magnetron sputtering. The as-prepared layers are then dewetted by the UV nanosecond laser pulses or alternatively in the furnace (temperature laser annealing and continuous thermal treatment in furnace can lead to the creation of NPs inside every Ti dimple and result in uniform coating of the whole area of structured templates. The size and localization of NPs obtained via both dewetting processes as well as their shape can be tuned by the annealing time and the laser processing parameters and also by initial thickness of Au layer and presence of the dimples themselves in the substrate. Results confirm that the prepared material can be used as substrate for SERS (Surface Enhanced Raman Spectroscopy).

  10. Gold Incorporated Mesoporous Silica Thin Film Model Surface as a Robust SERS and Catalytically Active Substrate

    Directory of Open Access Journals (Sweden)

    Anandakumari Chandrasekharan Sunil Sekhar

    2016-05-01

    Full Text Available Ultra-small gold nanoparticles incorporated in mesoporous silica thin films with accessible pore channels perpendicular to the substrate are prepared by a modified sol-gel method. The simple and easy spin coating technique is applied here to make homogeneous thin films. The surface characterization using FESEM shows crack-free films with a perpendicular pore arrangement. The applicability of these thin films as catalysts as well as a robust SERS active substrate for model catalysis study is tested. Compared to bare silica film our gold incorporated silica, GSM-23F gave an enhancement factor of 103 for RhB with a laser source 633 nm. The reduction reaction of p-nitrophenol with sodium borohydride from our thin films shows a decrease in peak intensity corresponding to –NO2 group as time proceeds, confirming the catalytic activity. Such model surfaces can potentially bridge the material gap between a real catalytic system and surface science studies.

  11. Legal-Ease The Annual Audit

    Institute of Scientific and Technical Information of China (English)

    CHRIS; DEVONSHIRE-ELLIS

    2007-01-01

    This is the fourth in a series of articles on 2007 audit requirements for wholly foreign-owned enterprises(WFOEs), foreign-invested enter-prises (FIEs) and joint ventures(JVs) in China.China uses a calendar fiscal year to impose statutory audits, so those for 2006 must be filed by April 2007 at the latest. By CHRIS DEVONSHIRE-ELLIS Preparation for the annual cooperative examination First of all, every company needs to apply for and obtain the annual cooperativeexaminationdocuments from thesame office of the State Administration for Industry and Commerce from which they have obtained their original business license.

  12. [NIR-SERS Spectra Detection of Cytidine on Nano-Silver Films].

    Science.gov (United States)

    Zhang, De-qing; Liu, Ren-ming; Zhang, Guo-qiang; Zhang, Yan; Xiong, Yang; Zhang, Chuan-yun; Li, Lun; Si, Min-zhen

    2016-03-01

    The polyvinyl alcohol (PVA) protected silver glass-like nanostructure (PVA-Ag-GNS) with high surface-enhanced Raman scattering (SERS) activity was prepared and employed to detect the near-infrared surface enhanced Raman scattering (NIR-SERS) spectra of cytidine aqueous solution (10(-2)-10(-8) mol x L(-1)). In the work, the near-infrared laser beam (785 nm) was used as the excitation light source. The experiment results show that high-quality NIR-SERS spectra were obtained in the ranges of 300 to 2 000 cm(-1) and the detection limit of cytidine aqueous solution was down to 10(-7) mol x L(-1). Meanwhile, the PVA-Ag-GNS shows a high enhancement factor (EF) of -10(8). In order to test the optical reproducibility of PVA-Ag-GNS, ten samples of cytidine aqueous solution (10(-2)-10(-5) mol x L(-1)) had been dropped onto the surface of PVA-Ag-GNS respectively. Meanwhile, these samples were measured by the portable Raman spectrometer. As a result, the PVA-Ag-GNS demonstrated good optical reproducibility in the detection of cytidine aqueous solution. In addition, to explain the reason of enhancement effect, the ultraviolet-visible (UV-Vis) extinction spectrum and scanning electron microscope (SEM) of cytidine molecules adsorbed on the surface of PVA-Ag-GNS were measured. There is plasmon resonance band at 800 nm in the UV-Vis extinction Spectrum of the compound system. Therefore, when the near-infrared laser beam (785 nm) was used as excitation light source, the compound system may produce strongly surface plasmon resonance (SPR). According to the SEM of PVA-Ag-GNS, there are much interstitial between the silver nanoparticles. So NIR-SERS is mainly attributed to electromagnetic (EM) fields associated with strong surface plasmon resonance. At last, the geometry optimization and pre-Raman spectrum of cytidine for the ground states were performed with DFT, B3LYP functional and the 6-311G basis set, and the near-infrared laser with wavelength of 785 nm was employed in the pre

  13. The Theory of SERS on Dielectrics and Semiconductors

    CERN Document Server

    Polubotko, V P Chelibanov A M

    2016-01-01

    It is demonstrated that the reason of SERS on dielectric and semiconductor substrates is the enhancement of the electric field in the regions of the tops of the surface roughness with very small radius, or a very large curvature. The enhancement depends on the dielectric constant of the substrate and is stronger for a larger dielectric constant. It is indicated that the enhancement on dielectrics and semiconductors is stronger than on metals with the same modulus of the dielectric constant. The result obtained is confirmed by experimental data on the enhancement coefficients obtained for various semiconductor and dielectric substrates.

  14. Fabricación aditiva con láser

    OpenAIRE

    Juan José de Damborenea González

    2015-01-01

    En menos de 30 años, la fabricación aditiva (en la literatura AM, Additive Manufacturing) la irrumpido con fuerza en el campo del procesamiento de materiales, especialmente desde 2007. De los diferentes métodos existentes para la fabricación de piezas por AM, el empleo de haces de alta densidad energética como el haz de electrones o el láser, se han mostrado particularmente valiosos por su capacidad para fabricar prototipos con diferentes composiciones y formas. La fabricación aditiva con lás...

  15. SERS-active nanoparticle aggregate technology for tags and seals

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Leif O [Los Alamos National Laboratory; Montoya, Velma M [Los Alamos National Laboratory; Havrilla, George J [Los Alamos National Laboratory; Doorn, Stephen K [Los Alamos National Laboratory

    2010-06-03

    In this paper, we describe our efforts to create a modern tagging and sealing technology for international safeguards application. Our passive tagging methods are based on SANAs (SERS-Active Nanoparticle Aggregates; SERS: Surface Enhanced Raman Scattering). These SANAs offer robust spectral barcoding capability in an inexpensive tag/seal, with the possibility of rapid in-field verification that requires no human input. At INMM 2009, we introduced SANAs, and showed approaches to integrating our technology with tags under development at Sandia National Laboratories (SNL). Here, we will focus on recent LANL development work, as well as adding additional dimensionality to the barcoding technique. The field of international safeguards employs a broad array of tags, seals, and tamper-indicating devices to assist with identification, tracking, and verification of components and materials. These devices each have unique strengths suited to specific applications, and span a range of technologies from passive metal cup seals and adhesive seals to active, remotely monitored fiber optic seals. Regardless of the technology employed, essential characteristics center around security, environmental and temporal stability, ease of use, and the ability to provide confidence to all parties. Here, we present a new inexpensive tagging technology that will deliver these attributes, while forming the basis of either a new seal, or as a secure layer added to many existing devices. Our approach uses the Surface Enhanced Raman Scattering (SERS) response from SANAs (SERS-Active Nanoparticle Aggregates, Figure 1) to provide a unique identifier or signature for tagging applications. SANAs are formed from gold or silver nanoparticles in the 40-80 nm size range. A chemical dye is installed on the nanoparticle surface, and the nanoparticles are then aggregated into ensembles of {approx}100 to 500 nm diameter, prior to being coated with silica. The silica shell protects the finished SANA from

  16. Los abusos sexuales: el riesgo de ser mujer

    OpenAIRE

    López Sánchez, Félix

    2005-01-01

    El objetivo de este artículo es revisar los estudios sobre abusos sexuales realizados nacional e internacionalmente, teniendo en cuenta la perspectiva de género. Todos los estudios analizados muestran de manera contundente que las niñas tienen el doble de posibilidades de ser víctimas que los niños, si bien ellos también lo son con frecuencia. Desde el punto de vista de los abusadores y agresores sexuales las diferencias de sexo son espectaculares: los abusadores y agresores...

  17. ¿Qué quiero ser de mayor?

    OpenAIRE

    Polo González, María Eugenia

    2011-01-01

    El escenario sociodemográfico actual hace que se dibuje un panorama novedoso en torno a la vejez que dista del de hace décadas. El hecho de que la jubilación no sea el final del camino, sino una etapa más de la vida induce, llegado el momento, a que el plantearse qué queremos ser de mayores cobre sentido. Algunos optan por el aprendizaje a lo largo de toda la vida, asistiendo, entre otras cosas, a la universidad. En el caso que nos ocupa, a esta circunstancia se añade la convivencia entre gen...

  18. Silver coated aluminium microrods as highly colloidal stable SERS platforms.

    Science.gov (United States)

    Pazos-Perez, Nicolas; Borke, Tina; Andreeva, Daria V; Alvarez-Puebla, Ramon A

    2011-08-01

    We report on the fabrication of a novel material with the ability to remain in solution even under the very demanding conditions required for structural and dynamic characterization of biomacromolecule assays. This stability is provided by the increase in surface area of a low density material (aluminium) natively coated with a very hydrophilic surface composed of aluminium oxide (Al(2)O(3)) and metallic silver nanoparticles. Additionally, due to the dense collection of active hot spots on their surface, this material offers higher levels of SERS intensity as compared with the same free and aggregated silver nanoparticles.

  19. EL PROCESO DE SER HISTERECTOMIZADA: ASPECTOS EDUCATIVOS A CONSIDERAR

    OpenAIRE

    Araya G,Alejandra; Urrutia S.,María Teresa

    2008-01-01

    Antecedentes: La histerectomía es una de las cirugías más frecuente en el mundo entero. La principal patología causante es la miomatosis uterina. A pesar de lo común de la cirugía, existe déficit en torno a la educación otorgada a este grupo de mujeres. Objetivo: El objetivo de la presente revisión bibliográfica es describir los aspectos que deben ser considerados al momento de educar a la mujer histerectomizada, específicamente las características que debiera tener el profesional, los conten...

  20. Radial system of dark globules in Ser OB2

    Science.gov (United States)

    Gyulbudaghian, A. L.

    2012-09-01

    A radial system illuminated by bright, central OB stars is discussed. 12CO(1-0) observations are made of three globules from this system. Three molecular clouds are detected which are dark globules of a radial system in Ser OB2. A group of IR stars near the boundary of a globule with a rim is studied. One of the IR stars with bright filaments is a YSO (young stellar object). The radial system contains two bright, Mshaped fronts located at the tip of a jet, which may have emerged from the Wolf-Rayet star WR113. Similar M-shaped fronts are also located near the object SNO 85.

  1. RECURSOS NO CONVENCIONALES SUSCEPTIBLES DE SER EXPLOTADOS MEDIANTE FRACKING

    OpenAIRE

    Jódar Abellán, Antonio

    2014-01-01

    En el presente trabajo se analizan en detalle los recursos de origen no convencional existentes en el contexto internacional, europeo, nacional y regional, que pueden ser explotados mediante fracking. Así mismo, se mencionará someramente la legislación aplicable a la fracturación hidráulica y los impactos, tanto beneficiosos como perjudiciales, que ésta puede acarrear. El estudio finaliza con una propuesta de posibles lugares en la Península Ibérica donde dicha técnica reporte mayores benefic...

  2. Aspectos sociales del documental “Ser y Tener”

    OpenAIRE

    Marín Gutiérrez, Isidro

    2010-01-01

    Después de haber expuesto a la clase los temas 5 (Sociedad, cultura y educación), el tema 6 (el proceso de socialización), tema 7 (estratificación, movilidad social y educación) y tema 8 (educación y control social) de sociología de la educación. Se pondrá el documental “Ser y Tener”. Posteriormente habrá un debate sobre el documental y sobre los conceptos sociológicos que en ella salgan.

  3. Annual Energy Review, 2008

    Energy Technology Data Exchange (ETDEWEB)

    None

    2009-06-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are statistics on total energy production, consumption, trade, and energy prices; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, and international energy; financial and environment indicators; and data unit conversions.

  4. CSIR Annual report 1993

    CSIR Research Space (South Africa)

    CSIR

    1993-01-01

    Full Text Available stream_source_info Annual Report_ 1993.pdf.txt stream_content_type text/plain stream_size 45 Content-Encoding ISO-8859-1 stream_name Annual Report_ 1993.pdf.txt Content-Type text/plain; charset=ISO-8859-1 ...

  5. CSIR Annual report 1974

    CSIR Research Space (South Africa)

    CSIR

    1974-01-01

    Full Text Available stream_source_info Annual Report_1974.pdf.txt stream_content_type text/plain stream_size 89 Content-Encoding ISO-8859-1 stream_name Annual Report_1974.pdf.txt Content-Type text/plain; charset=ISO-8859-1 ...

  6. CSIR Annual report 1972

    CSIR Research Space (South Africa)

    CSIR

    1972-01-01

    Full Text Available stream_source_info Annual Report_ 1972.pdf.txt stream_content_type text/plain stream_size 95 Content-Encoding ISO-8859-1 stream_name Annual Report_ 1972.pdf.txt Content-Type text/plain; charset=ISO-8859-1 ...

  7. CSIR Annual report 1975

    CSIR Research Space (South Africa)

    CSIR

    1975-01-01

    Full Text Available stream_source_info Annual Report_1975.pdf.txt stream_content_type text/plain stream_size 101 Content-Encoding ISO-8859-1 stream_name Annual Report_1975.pdf.txt Content-Type text/plain; charset=ISO-8859-1 ...

  8. CSIR Annual report 1979

    CSIR Research Space (South Africa)

    CSIR

    1979-01-01

    Full Text Available stream_source_info CSIR Annual report_1979.pdf.txt stream_content_type text/plain stream_size 86 Content-Encoding ISO-8859-1 stream_name CSIR Annual report_1979.pdf.txt Content-Type text/plain; charset=ISO-8859-1 ...

  9. CSIR Annual report 1978

    CSIR Research Space (South Africa)

    CSIR

    1978-01-01

    Full Text Available stream_source_info Annual Report_1978.pdf.txt stream_content_type text/plain stream_size 78 Content-Encoding ISO-8859-1 stream_name Annual Report_1978.pdf.txt Content-Type text/plain; charset=ISO-8859-1 ...

  10. Annual Review of Biophysics.

    Science.gov (United States)

    Hatzis, Christos

    2013-07-01

    Annual Review of Biophysics Rees D. Dill K., Williamson J., Annual Reviews Palo Alto, CA, 2010. 581 pp. (hardcover), ISBN: 978-0-8243-1839-0, © 2013 Doody's Review Service. Doody's Review Service. © 2013 American Association of Physicists in Medicine.

  11. Annual Report 1997

    Energy Technology Data Exchange (ETDEWEB)

    Golnik, N.; Mika, J.R.; Wieteska, K. [eds.

    1998-12-31

    This Annual Report of the Institute of Atomic Energy describes the results of the research works carried out at the Institute at 1997. As in the preceding years the authors of the individual scientific reports published in this Annual Report are fully responsible for their content and layout. The Report contains the information on other activities of the Institute as well. (author)

  12. Fabrication of ITO-rGO/Ag NPs nanocomposite by two-step chronoamperometry electrodeposition and its characterization as SERS substrate

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Rong [Chemistry and Chemical Engineering College, Chongqing University, Shapingba, Chongqing 400044 (China); Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); Analytical and Testing Center, Sichuan University of Science & Engineering, Zigong, Sichuan 643000 (China); Xu, Yi [Chemistry and Chemical Engineering College, Chongqing University, Shapingba, Chongqing 400044 (China); Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); Wang, Chunyan [Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); School of Optoelectronic Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); Zhao, Huazhou; Wang, Renjie; Liao, Xin [Chemistry and Chemical Engineering College, Chongqing University, Shapingba, Chongqing 400044 (China); Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); Chen, Li; Chen, Gang [Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); School of Optoelectronic Engineering, Chongqing University, Shapingba, Chongqing 400044 (China)

    2015-09-15

    Highlights: • A novel structure of ITO-rGO/Ag NPs substrate was developed for SERS application. • Two-step chronoamperometry deposition method was used to prepare SERS substrate. • The SERS substrate had high SERS activity, good uniformity and reproducibility. - Abstract: A novel composite structure of reduced graphene oxide (rGO)–Ag nanoparticles (Ag NPs) nanocomposite, which was integrated on the indium tin oxide (ITO) glass by a facile and rapid two-step chronoamperometry electrodeposition route, was proposed and developed in this paper. SERS-activity of the rGO/Ag NPs nanocomposite was mainly affected by the structure and size of the fabricated rGO/Ag NPs nanocomposite. In the experiments, the operational conditions of electrodeposition process were studied in details. The electrodeposited time was the important controllable factor, which decided the particle size and surface coverage of the deposited Ag NPs on ITO glass. Under the optimized conditions, the detection limit for rhodamine6G (R6G) was as low as 10{sup −11} M and the Raman enhancement factor was as large as 5.9 × 10{sup 8}, which was 24 times higher than that for the ITO–Ag NPs substrate. Apart from this higher enhancement effect, it was also illustrated that extremely good uniformity and reproducibility with low standard deviation could be obtained by the prepared ITO-rGO/Ag NPs nanocomposite for SRES detection.

  13. Graphene oxide-Ag nanoparticles-pyramidal silicon hybrid system for homogeneous, long-term stable and sensitive SERS activity

    Science.gov (United States)

    Guo, Jia; Xu, Shicai; Liu, Xiaoyun; Li, Zhe; Hu, Litao; Li, Zhen; Chen, Peixi; Ma, Yong; Jiang, Shouzhen; Ning, Tingyin

    2017-02-01

    In our work, few layers graphene oxide (GO) were directly synthesized on Ag nanoparticles (AgNPs) by spin-coating method to fabricate a GO-AgNPs hybrid structure on a pyramidal silicon (PSi) substrate for surface-enhanced Raman scattering (SERS). The GO-AgNPs-PSi substrate showed excellent Raman enhancement effect, the minimum detected concentration for Rhodamine 6G (R6G) can reach 10-12 M, which is one order of magnitude lower than the AgNPs-PSi substrate and two order of magnitude lower than the GO-AgNPs-flat-Si substrate. The linear fit calibration curve with error bars is presented and the value of R2 of 612 and 773 cm-1 can reach 0.986 and 0.980, respectively. The excellent linear response between the Raman intensity and R6G concentrations prove that the prepared GO-AgNPs-PSi substrates can serve as good SERS substrate for molecule detection. The maximum deviations of SERS intensities from 20 positions of the GO-AgNPs-PSi substrate are less than 8%, revealing the high homogeneity of the SERS substrate. The excellent homogeneity of the enhanced Raman signals can be attributed to well-separated pyramid arrays of PSi, the uniform morphology of AgNPs and multi-functions of GO layer. Besides, the uniform GO film can effectively protect AgNPs from oxidation and endow the hybrid system a good stability and long lifetime. This GO-AgNPs-PSi substrate may provide a new way toward practical applications for the ultrasensitive and label-free SERS detection in areas of medicine, food safety and biotechnology.

  14. Reporter-Embedded SERS Tags from Gold Nanorod Seeds: Selective Immobilization of Reporter Molecules at the Tip of Nanorods.

    Science.gov (United States)

    Wang, Yuan; Wang, Yunqing; Wang, Wenhai; Sun, Kaoxiang; Chen, Lingxin

    2016-10-03

    Reporter embedded (RE) tags are a new generation of sensitive, stable surface enhanced Raman scattering (SERS) tags with Raman reporters embedded between gold nanoparticle (NP) cores and gold (or silver) shells. Most of the reported RE tags have been designed using Au nanospheres as a seed material. Herein, we investigated the synthesis and SERS properties of AuNR/reporter/Ag tags by using gold nanorod (AuNR) seeds with anisotropic physical and optical features. Several highlighted points were discovered, including the following: 1) The cetyltrimethylammonium bromide (CTAB) layer induced the co-existence of chemically and physically adsorbed Raman reporters on AuNR. Conventional washing of the AuNR-reporter complex with water results in the formation of an "internal-external" mixed tag. To obtain a "pure" RE structure, an additional extraction step involving a CTAB solution was essential. 2) The anisotropic distribution of CTAB on AuNR resulted in the preference of the Raman reporters to adsorb to the hotspot at AuNR tip, which made it a perfect match for improving the SERS signal of the tag. 3) An anisotropic silver coating occurred with the shell thickness on the AuNR side growing much faster than the shell thickness at the tip. This feature ensured that the tag grew to a suitable size with enough silver for SERS enhancement without shadowing the effective Raman reporters at the tip too much. 4) RE tags showed better in vitro and in vivo signal stabilities compared with their external labeling counterparts. Moreover, a novel pH-sensitive SERS peak test was proposed by using 4-mercaptobenzoic acid as the Raman reporter to verify thin coverage by a silver layer. We believe this tag can be broadly applied for molecular detection and bio-imaging, and the proposed preparation and structure verification methods can provide universal guidance in the design of novel RE tags.

  15. Development and precise characterization of phospho-site-specific antibody of Ser(357) of IRS-1: elimination of cross reactivity with adjacent Ser(358).

    Science.gov (United States)

    Waraich, Rizwana Sanaullah; Zaidi, Nousheen; Moeschel, Klaus; Beck, Alexander; Weigert, Cora; Voelter, Wolfgang; Kalbacher, Hubert; Lehmann, Rainer

    2008-11-07

    Antibodies that recognize specifically phosphorylated sites on proteins are widely utilized for studying the regulation and biological function of phosphoproteins. The proposed strategy is a powerful, analytical tool allowing the generation of phospho-site specific antibodies albeit adjacent phosphorylation sites are present. Here, we demonstrate the assessment and elimination of cross reactivity of phospho-site-specific-Ser(357) IRS-1 antibody. While determining the specificity of p-Ser(357) antiserum we came across the cross reactivity of the antiserum with adjacent Ser(358) which was successfully abolished by an improved immuno-purification method. The specificity of the purified antiserum was then verified by indirect ELISA, results of ELISA were also mirrored in the experiments carried out in BHK-IR cells using different mutants of IRS-1 carrying mutations at either Ser(357)/Ser(358)/Ser(357/358). Immuno-purified-p-Ser(357) did not react with IRS-1 Ala(357) and IRS-1 Ala(357/358). In conclusion, the present study describes generation and characterization of p-Ser(357) IRS-1 antibody, which reacts with IRS-1 in site specific and phosphorylation state-dependent manner without showing cross reactivity to adjacent Ser(358). This antibody can be effectively used to further clarify the inhibitory role of Ser(357) in insulin signal transduction.

  16. Using Standing Gold Nanorod Arrays as Surface-Enhanced Raman Spectroscopy (SERS) Substrates for Detection of Carbaryl Residues in Fruit Juice and Milk.

    Science.gov (United States)

    Alsammarraie, Fouad K; Lin, Mengshi

    2017-01-25

    In recent years, there have been increasing concerns about pesticide residues in various foods. On the other hand, there is growing attention in utilizing novel nanomaterials as highly sensitive, low-cost, and reproducible substrates for surface-enhanced Raman spectroscopy (SERS) applications. The objective of this study was to develop a SERS method for the rapid detection of pesticides that were extracted from different types of food samples (fruit juice and milk). A new SERS substrate was prepared by assembling gold nanorods into standing arrays on a gold-coated silicon slide. The standing nanorod arrays were neatly arranged and were able to generate a strong electromagnetic field in SERS measurement. The as-prepared SERS substrate was utilized to detect carbaryl in acetonitrile/water solution, fruit juices (orange and grapefruit), and milk. The results show that the concentrations of carbaryl spiked in fruit juice and milk were linearly correlated with the concentrations predicted by the partial least-squares (PLS) models with r values of 0.91, 0.88, and 0.95 for orange juice, grapefruit juice, and milk, respectively. The SERS method was able to detect carbaryl that was extracted from fruit juice and milk samples at a 50 ppb level. The detection limits of carbaryl were 509, 617, and 391 ppb in orange juice, grapefruit juice, and milk, respectively. All detection limits are below the maximum residue limits that were set by the U.S. EPA. Moreover, satisfactory recoveries (82-97.5%) were accomplished for food samples using this method. These results demonstrate that SERS coupled with the standing gold nanorod array substrates is a rapid, reliable, sensitive, and reproducible method for the detection of pesticide residues in foods.

  17. In situ SERS monitoring of photocatalytic organic decomposition using recyclable TiO{sub 2}-coated Ag nanowire arrays

    Energy Technology Data Exchange (ETDEWEB)

    Bao, Zhi Yong [Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong (China); Liu, Xin [Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong (China); Key Lab of Advanced Transducers and Intelligent Control System of Ministry of Education, and College of Physics and Optoelectronics, Taiyuan University of Technology, Taiyuan (China); Dai, Jiyan [Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong (China); Shenzhen Research Institute, The Hong Kong Polytechnic University, Shenzhen (China); Wu, Yucheng [College of Material Science and Engineering, Hefei University of Technology, Hefei (China); Tsang, Yuen Hong, E-mail: yuen.tsang@polyu.edu.hk [Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong (China); Lei, Dang Yuan, E-mail: dylei@polyu.edu.hk [Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong (China); Shenzhen Research Institute, The Hong Kong Polytechnic University, Shenzhen (China)

    2014-05-01

    Highlights: • TiO{sub 2}-coated Ag nanowire arrays (SNA) were synthesized through hydrolysis of butyl titanate on the surface of SNA. • The TiO{sub 2}-coated SNA exhibits much higher photocatalytic activity than bare TiO{sub 2} films, as demonstrated by the enhanced photodegradation rate of R6G and methyl blue molecules. • The prepared substrates serve as a high-sensitivity surface-enhanced Raman scattering (SERS) substrate for in situ monitoring of the photocatalytic decomposition reaction detecting 2,4-D molecules. • Full-wave numerical calculations reveal that the improved photocatalysis and SERS efficiencies are attributed to the largely enhanced electromagnetic near-field in the nanocomposites. - Abstract: Recently, semiconductor and noble metal complex nanomaterials have attracted ever-increasing attention because of the realization of multiple functionalities in a single entity. In this study, Ag–TiO{sub 2} core–shell nanocomposites were synthesized by hydrolysis of butyl titanate on the surface of Ag nanowires. Due to efficient electron transfer at the Ag–TiO{sub 2} interface, the as-prepared nanocomposites exhibit much higher photocatalytic activity than bare TiO{sub 2} films, as demonstrated by the enhanced photodegradation rate of R6G and methyl blue molecules. In the meanwhile, such nanocomposites serve as a high-sensitivity surface-enhanced Raman scattering (SERS) substrate for in situ monitoring of the photocatalytic decomposition reaction, and the substrate is recyclable due to its self-cleaning function. Full-wave numerical calculations reveal that the improved photocatalysis and SERS efficiencies are attributed to the largely enhanced electromagnetic near-field in the nanocomposites. Our results point out that bifunctional semiconductor-metal hybrids hold great promise for simultaneously detecting and decomposing organic pollutants in the environment.

  18. Immunoassay for tumor markers in human serum based on Si nanoparticles and SiC@Ag SERS-active substrate.

    Science.gov (United States)

    Zhou, Lu; Zhou, Jun; Feng, Zhao; Wang, Fuyan; Xie, Shushen; Bu, Shizhong

    2016-04-21

    Based on a sandwich structure consisting of nano-Si immune probes and a SiC@Ag SERS-active immune substrate, a kind of ultra-sensitive immunoassay protocol is presented to detect tumor markers in human serum. The nano-Si immune probes were prepared by immobilizing the detecting antibodies onto the surfaces of SiO2-coated Si nanoparticles (NPs) which were modified with 3-(aminopropyl)trimethoxysilane, and the SiC@Ag SERS-active immune substrates were prepared by immobilizing the captured antibodies on Ag film sputtered on SiC sandpaper. To the best of our knowledge, it is the first time that Si NPs are directly used as Raman tags in an immunoassay strategy. And, the SiC@Ag SERS-active substrates exhibit excellent surface enhanced Raman scattering (SERS) performances with an enhancement factor of ∼10(5), owing to the plasmonic effect of the Ag film on the rough surface of the SiC sandpaper. In our experiments, the sandwich immunoassay structure has been successfully applied to detect prostate specific antigen (PSA), α-fetoprotein (AFP) and carbohydrate antigen 19-9 (CA19-9) in a human serum sample and the limit of detections are as low as 1.79 fg mL(-1), 0.46 fg mL(-1) and 1.3 × 10(-3) U mL(-1), respectively. It reveals that the proposed immunoassay protocol has demonstrated a high sensitivity for tumor markers in human serum and a potential practicability in biosensing and clinical diagnostics.

  19. LAS CONSECUENCIAS JURÍDICAS DE NO SER UNA PROVINCIA*

    Directory of Open Access Journals (Sweden)

    Leonardo Hugo Limanski

    2015-09-01

    Full Text Available En el presente trabajo se analiza, en forma profunda, la autonomía de la Ciudad Autónoma de Buenos Aires, en comparación con las 23 provincias argentinas. A su vez, se intentará demostrar que la Ciudad de Buenos Aires debe ser tratada en forma equivalente a una provincia, desarrollando los problemas que la interpretación actual de la Constitución Nacional por parte de la Corte Suprema acarrea. A los efectos de lograr una cabal comprensión del tema se realizará una breve reseña histórica partiendo desde la federalización en el año 1880, y se hará hincapié en aquellos temas que, creo, violan la igualdad. Es así que se pondrán de manifiesto cuales son las consecuencias jurídicas de no ser considerada una provincia y, por último, se realizará una aproximación al vínculo existente entre las comunas y los municipios, a efectos de dejar planteado un problema

  20. Development of an optofluidic SERS-based biomedical sensor

    Science.gov (United States)

    Walton, Brian; Huang, Po-Jung; Kameoka, Jun; Deutz, Nicolaas; Coté, Gerard L.

    2016-03-01

    Rapid assessment of radiation exposure to sensitive organs like the gut is extremely important for large populations exposed to ionized radiation, for instance during warfare. Recent results have shown that plasma citrulline levels appear to track gut function after irradiation levels in mice and humans. The current ways to monitor blood citrulline levels are bulky, laborious, time-consuming and expensive methods. Therefore, an optofludic point-of-care (POC) system using surface enhanced Raman spectroscopy to measure plasma citrulline as a marker for radiation exposure that overcomes the above issues is being developed. As a first step toward development of this system four colloidal nanoparticles, spherical gold, silver cubes, silica-gold nanoshells, and silver-gold nanocages have been analyzed for use in the POC system. Transmission electron microscopy (TEM) images have been taken of each nanoparticle to visualize the morphology of the nanoparticles, which is vital for SERS. Ultraviolet-visible (UV/Vis) spectroscopy was also collected to verify the extinction spectra for each nanoparticle was in resonance with the excitation wavelength. The nanoparticles were functionalized with mercaptobenzoic acid (MBA), a Raman reporter molecule, and SERS spectra were collected to determine which has better utility in a novel micro-to-nanochannel. The data showed that the silver nanocubes have a larger enhancement factor than the gold nanospheres, nanoshells, or nanocages. Currently, these nanocubes are being functionalized with the citulline for assessing the concentration sensitivity and dynamic range for ultimate use as a marker for radiation.

  1. Idaho National Laboratory PCB Annual Document Log and Annual Records Report for calendar year 2014

    Energy Technology Data Exchange (ETDEWEB)

    Layton, Deborah L. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-06-01

    The requirements for the reporting of polychlorinated biphenyl (PCB)-related activities are found in 40 Code of Federal Regulations (CFR) 761 Subpart J, "General Records and Reports." The PCB Annual Document Log is a detailed record of the PCB waste handling activities at the facility. The facility must prepare it each year by July 1 and maintain it at the facility for at least 3 years after the facility ceases using or storing PCBs and PCB items. While submittal of the PCB Annual Document Log to the U.S. Environmental Protection Agency (EPA) is not required by regulation, EPA has verbally requested in telephone conversations that this report be submitted to them on an annual basis. The Annual Records are not required to be submitted to EPA and are not considered to be part of the Annual Document Log, but are included to provide the complete disposition history or status of all PCB activities during the year. The Annual Document Log section of this report (Section 2.0) meets the requirements of 40 CFR 761.180(a)(2), as applicable, while the Annual Records section (Section 3.0) meets the requirement of 40 CFR 761.180(a)(1).

  2. Based on time and spatial-resolved SERS mapping strategies for detection of pesticides.

    Science.gov (United States)

    Ma, Bingbing; Li, Pan; Yang, Liangbao; Liu, Jinhuai

    2015-08-15

    For the sensitive and convenient detection of pesticides, several sensing methods and materials have been widely explored. However, it is still a challenge to obtain sensitive, simple detection techniques for pesticides. Here, the simple and sensitive Time-resolved SERS mapping (T-SERS) and Spatial-resolved SERS mapping (S-SERS) are presented for detection of pesticides by using Au@Ag NPs as SERS substrate. The Time-resolved SERS mapping (T-SERS) is based on state translation nanoparticles from the wet state to the dry state to realize SERS measurements. During the SERS measurement, adhesive force drives the particles closer together and then average interparticle gap becomes smaller. Following, air then begins to intersperse into the liquid network and the particles are held together by adhesive forces at the solid-liquid-air interface. In the late stage of water evaporation, all particles are uniformly distributed. Thus, so called hotspots matrix that can hold hotspots between every two adjacent particles in efficient space with minimal polydispersity of particle size are achieved, accompanying the red-shift of surface plasmon peak and appearance of an optimal SPR resonated sharply with excitation wavelength. Here, we found that the T-SERS method exhibits the detection limits of 1-2 orders of magnitude higher than that of S-SERS. On the other hand, the T-SERS is very simple method with high detection sensitivity, better reproducibility (RSD=10.8%) and is beneficial to construction of a calibration curve in comparison with that of Spatial-resolved SERS mapping (S-SERS). Most importantly, as a result of its remarkable sensitivity, T-SERS mapping strategies have been applied to detection of several pesticides and the detect limit can down to 1nM for paraoxon, 0.5nM for sumithion. In short, T-SERS mapping measurement promises to open a market for SERS practical detection with prominent advantages.

  3. Annual Coded Wire Program Missing Production Groups, 1996 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Pastor, S.M. [Fish and Wildlife Service, Vancouver, WA (United States). Columbia River Fisheries Program Office

    1997-07-01

    In 1989 the Bonneville Power Administration (BPA) began funding the evaluation of production groups of juvenile anadromous fish not being coded-wire tagged for other programs. These groups were the ``Missing Production Groups``. Production fish released by the US Fish and Wildlife Service (USFWS) without representative coded-wire tags during the 1980`s are indicated as blank spaces on the survival graphs in this report. The objectives of the ``Missing Production Groups`` program are: to estimate the total survival of each production group, to estimate the contribution of each production group to various fisheries, and to prepare an annual report for all USFWS hatcheries in the Columbia River basin. Coded-wire tag recovery information will be used to evaluate the relative success of individual brood stocks. This information can also be used by salmon harvest managers to develop plans to allow the harvest of excess hatchery fish while protecting threatened, endangered, or other stocks of concern.

  4. Investigations on NIR-SERS Spectra of Serum for Liver Cancer Based on NIR-SERS Substrate%基于新型NIR-SERS基底的肝癌血清NIR-SERS光谱研究

    Institute of Scientific and Technical Information of China (English)

    刘仁明; 刘瑞明; 武延春; 柳振全; 张德清; 自兴发; 司民真

    2011-01-01

    Investigations on near infrared surface-enhanced Raman scattering (NIR-SERS) spectra of the serum for patients with liver cancer and healthy persons based on Ag nanofilms prepared by using electrostatic self-assembly are reported. Analysis indicates NIR-SERS spectra of the sera between healthy persons and liver cancer patients are different. Firstly, Raman bands at 630, 720, 812 and 1578 cm-1 become weaker (even disappear) in NIR-SERS spectra of the sera for cancer liver patients but stronger in NIR-SERS spectra of the sera for healthy persons.Secondly, Raman bands at 1130 cm-1 and 1204 cm-1 in NIR-SERS spectra of the sera for healthy persons have blue shifts to 1135 cm-1 and 1269 cm 1 in NIR-SERS spectra of sera for cancer liver patients, respectively. Meanwhile, a new Raman band at 558 cm-1 appears in NIR-SERS spectra of liver cancer patients. Additionally, striking spectral differences are abvious in NIR-SERS spectra in the intensity ratios at 630/300, 1130/300, and 1578/300 cm-1.These three peak-intensity ratios of liver cancer patients with values of 0. 848±0. 042, 1. 094~ 0. 118, 0. 914 ± 0. 070, respectively, are more notable (mean ~ S. D., n = 15, P < 0.01 ) compared with those of healthy persons (1.985t0. 487, 1.568±0.286, 1. 189±0. 108, respectively). The results show that the intensity relative peak-ratios at 630/300, 1130/300, and 1578/300 cm-1 can be used to discriminate liver cancer patients from healthy persons, which indicate these three intensity ratios can be served as N1R-SERS spectral criteria for the diagnosis of cancer liver.%基于一种新型、高效、生物兼容性近红外表面增强拉曼散射(NIR-SERS)基底,采用便携式近红外拉曼光谱仪分别对健康人和肝癌病患者的血清进行了NIR-SERS光谱研究.实验发现,健康人与肝癌患者的血清NIR-SERS光谱存在显著差异:1)健康人血清NIR-SERS光谱中位于630、720、812和1578 cm-1附近的谱峰在肝癌患者血清NIR-SERS光

  5. P21 Ser31Arg polymorphism and cervical cancer risk: a meta-analysis.

    Science.gov (United States)

    Li, Ya; Liu, Fei; Tan, Shiqiao; Li, Shangwei

    2011-04-01

    Studies investigating the association between p21 genetic polymorphism Ser31Arg and cervical cancer (CC) risk report conflicting results. The aim of this study was to quantitatively summarize the evidence for p21 polymorphism and CC risk. Two investigators independently searched the MEDLINE, Embase, CNKI, and Chinese Biomedicine databases. Summary odds ratios (ORs) and 95% confidence intervals (CIs) for p21 polymorphism and CC were calculated in a fixed effects model (the Mantel-Haenszel method) and a random effects model (the DerSimonian and Laird method) when appropriate. The pooled ORs were performed for codominant model (Arg/Arg vs Ser/Ser and Arg/Ser vs Ser/Ser), dominant model (Arg/Arg + Arg/Ser vs Ser/Ser), and recessive model (Arg/Arg vs Arg/Ser + Ser/Ser). Subgroup analyses were performed by country, matched controls, and Hardy-Weinberg equilibrium in the controls and study sample size. This meta-analysis included 10 case-control studies from an Asian population, which included 1415 CC cases and 1947 controls. Overall, the variant genotypes (Arg/Arg and Arg/Ser) of Ser31Arg were not associated with CC risk, when compared with the wild-type homozygote Ser/Ser (Arg/Arg vs Ser/Ser: OR, 1.30; 95% CI, 0.81-2.08; Arg/Ser vs Ser/Ser: OR, 1.06; 95% CI, 0.72-1.55). Similarly, no associations were found in the dominant and recessive models (dominant model: OR, 1.05; 95% CI, 0.73-1.51; recessive model: OR, 1.28; 95% CI, 0.86-1.90). Stratified analyses also detected no significant association in any subgroup, except among those studies from "other" country and those studies with controls deviated from Hardy-Weinberg equilibrium. No association was found between the p21 polymorphism Arg31Ser and risk of CC among Asians. In the future, additional studies based on white and African American patients should be performed to re-evaluate the association.

  6. Parkin is activated by PINK1-dependent phosphorylation of ubiquitin at Ser65

    Science.gov (United States)

    Kazlauskaite, Agne; Kondapalli, Chandana; Gourlay, Robert; Campbell, David G.; Ritorto, Maria Stella; Hofmann, Kay; Alessi, Dario R.; Knebel, Axel; Trost, Matthias; Muqit, Miratul M. K.

    2014-01-01

    We have previously reported that the Parkinson's disease-associated kinase PINK1 (PTEN-induced putative kinase 1) is activated by mitochondrial depolarization and stimulates the Parkin E3 ligase by phosphorylating Ser65 within its Ubl (ubiquitin-like) domain. Using phosphoproteomic analysis, we identified a novel ubiquitin phosphopeptide phosphorylated at Ser65 that was enriched 14-fold in HEK (human embryonic kidney)-293 cells overexpressing wild-type PINK1 stimulated with the mitochondrial uncoupling agent CCCP (carbonyl cyanide m-chlorophenylhydrazone), to activate PINK1, compared with cells expressing kinase-inactive PINK1. Ser65 in ubiquitin lies in a similar motif to Ser65 in the Ubl domain of Parkin. Remarkably, PINK1 directly phosphorylates Ser65 of ubiquitin in vitro. We undertook a series of experiments that provide striking evidence that Ser65-phosphorylated ubiquitin (ubiquitinPhospho−Ser65) functions as a critical activator of Parkin. First, we demonstrate that a fragment of Parkin lacking the Ubl domain encompassing Ser65 (ΔUbl-Parkin) is robustly activated by ubiquitinPhospho−Ser65, but not by non-phosphorylated ubiquitin. Secondly, we find that the isolated Parkin Ubl domain phosphorylated at Ser65 (UblPhospho−Ser65) can also activate ΔUbl-Parkin similarly to ubiquitinPhospho−Ser65. Thirdly, we establish that ubiquitinPhospho−Ser65, but not non-phosphorylated ubiquitin or UblPhospho−Ser65, activates full-length wild-type Parkin as well as the non-phosphorylatable S65A Parkin mutant. Fourthly, we provide evidence that optimal activation of full-length Parkin E3 ligase is dependent on PINK1-mediated phosphorylation of both Parkin at Ser65 and ubiquitin at Ser65, since only mutation of both proteins at Ser65 completely abolishes Parkin activation. In conclusion, the findings of the present study reveal that PINK1 controls Parkin E3 ligase activity not only by phosphorylating Parkin at Ser65, but also by phosphorylating ubiquitin at Ser65

  7. Sandwich-like layer-by-layer assembly of gold nanoparticles with tunable SERS properties

    Directory of Open Access Journals (Sweden)

    Zhicheng Liu

    2016-07-01

    Full Text Available Sandwich-like layer-by-layer thin films consisting of polyelectrolytes and gold nanoparticles were utilized to construct surface-enhanced Raman scattering (SERS substrates with tunable SERS properties. It is found that both the size of the nanoparticles in the layers and the interlayer distance significantly influence the SERS performance of the multilayered thin film. These simple, low-cost, easily processable and controllable SERS substrates have a promising future in the field of molecular sensing.

  8. Gold nanoparticle-coated biomaterial as SERS micro-probes

    Indian Academy of Sciences (India)

    G V Pavan Kumar

    2011-06-01

    We report for the first time, on the utility of plant-based biomaterial as enhanced-Raman scattering probes. The bio-substrate used in this study are commonly found in plant extracts, and are cost-effective, mechanically robust, flexible and easily transportable. The probe was fabricated by coating the plant extract with gold nanoparticles and characterized. By employing a ‘single-touch contact’ method, we reveal the ability of these probes to detect routinely used Raman markers such as 2-napthalenethiol and rhodamine B, at nano-molar concentrations, in dry and liquid forms, respectively. Reproducibility of the signals with variation <5%, and the ability to detect biomolecules are demonstrated herein. We envision these bio-probes as potential candidates for enhanced Raman sensing in chemical, environmental, and archaeological applications. By further engineering the shape, morphology, and surface chemistry of these micro-probes, we foresee their utility as miniaturized, natural SERS substrates.

  9. La razón de ser de los conglomerantes

    Directory of Open Access Journals (Sweden)

    Fernández Paris, J. M.

    1978-09-01

    Full Text Available La palabra cemento sirve para designar cualquier sustancia con capacidad adherente, mientras que el término conglomerante se aplica a la acción de aglutinar en un todo partículas que anteriormente estaban sueltas o independientes. Según su capacidad o no de endurecer bajo agua, los conglomerantes se clasifican en hidráulicos y no hidráulicos. Se denominan hidráulicos a aquéllos que tienen la citada facultad. Entre todos los conglomerantes hidráulicos, han adquirido en la construcción una carta de naturaleza inusitada durante los últimos tiempos los denominados cementos porland. Quiero hacer constar que trato de exponer simplemente aquellos conceptos químicos que, a mi modo de ver y de acuerdo con mi experiencia, constituyen la razón de ser que nos permita apreciar sus cualidades y propiedades.

  10. Au-Ag hollow nanostructures with tunable SERS properties

    Science.gov (United States)

    Jiji, S. G.; Gopchandran, K. G.

    2017-01-01

    Fabrication of hollow Au-Ag nanoparticles is done by the sequential action of galvanic replacement and Kirkendall effect. Polyol synthesized silver nanoparticles were used as templates and the size of cavities is controlled by the systematic addition of the HAuCl4. Au-Ag nanoparticles carved in different depths were tested for application as substrates for surface enhanced Raman scattering. Two medically important Raman active analytes-Nile blue chloride and Crystal violet were used in the surface enhanced Raman scattering (SERS) performance analysis. A systematic study has been made on the Raman enhancement of hollow nanoparticles fabricated with different cavity dimensions and compared with that of the silver templates used. The enhancement observed for these hollow substrates with cavities is of interest since Au protected hollow nanostructures are vital and an active area of interest in drug delivery systems.

  11. Nanofabrication of SERS Substrates for Single/Few Molecules Detection

    KAUST Repository

    Melino, Gianluca

    2015-05-04

    Raman spectroscopy is among the most widely employed methods to investigate the properties of materials in several fields of study. Evolution in materials science allowed us to fabricate suitable substrates, at the nanoscale, capable to enhance the electromagnetic field of the signals coming from the samples which at this range turn out to be in most cases singles or a few molecules. This particular variation of the classical technique is called SERS (Surface Enanched Raman Spectroscopy). In this work, the enhancement of the electromagnetic field is obtained by manipulation of the optical properties of metals with respect to their size. By using electroless deposition (bottom up technique), gold and silver nanoparticles were deposited in nanostructured patterns obtained on silicon wafers by means of electron beam lithography (top down technique). Rhodamine 6G in aqueous solution at extremely low concentration (10-8 M) was absorbed on the resultant dimers and the collection of the Raman spectra demonstrated the high efficiency of the substrates.

  12. The World Bank Annual Report 2010

    OpenAIRE

    World Bank

    2010-01-01

    The Annual Report is prepared by the Executive Directors of the International Bank for Reconstruction and Development (IBRD) and the International Development Association (IDA) in accordance with the by-laws of the two institutions. The President of the IBRD and IDA and the Chairman of the Boards of Executive Directors submit the Report, together with the accompanying administrative budgets and audited financial statements, to the Board of Governors.

  13. The World Bank Annual Report 2016

    OpenAIRE

    World Bank

    2016-01-01

    The Annual Report is prepared by the Executive Directors of the International Bank for Reconstruction and Development (IBRD) and the International Development Association (IDA)--collectively known as the World Bank--in accordance with the by-laws of the two institutions. The President of the IBRD and IDA and the Chairman of the Board of Executive Directors submits the Report, together with the accompanying administrative budgets and audited financial statements, to the Board of Governors.

  14. LLNL NESHAPs 1996 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Gallegos, G.M.

    1997-01-06

    This annual report is prepared pursuant to the National Emissions Standards for Hazardous Air Pollutants (NESHAPs) 40 CFR Part 61, Subpart H; Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an annual effective dose equivalent (EDE) of 10 mrem (10 microsieverts) to any member of the public. The EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from 1996 operations were (1) Livermore site: 0. 093 mrem (0.93 microsievert) (52% from point-source emissions, 48% from diffuse-source emissions); (2) Site 300: 0.033 mrem (0.33 microsievert) (99% from point-source, 1% from diffuse-source emissions). The EDEs were generally calculated using the EPA-approved CAP88-PC air-dispersion/dose-assessment model. Site-specific meteorological data, stack flow data, and emissions estimates based on radionuclide inventory data or continuous-monitoring systems data were the specific input to CAP88-PC for each modeled source. 5 figs., 8 tabs.

  15. LLNL NESHAPs 2002 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Harrach, R J; Gallegos, G M; Peterson, S-R; Tate, P J; Bertoldo, N A; Wilson, K R; Althouse, P E; Larson, J M

    2003-06-01

    This annual report is prepared pursuant to the National Emission Standards for Hazardous Air Pollutants (NESHAPs; Title 40 Code of Federal Regulations [CFR] Part 61, Subpart H). Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an annual effective dose equivalent (EDE) of 10 mrem (100 {micro}Sv) to any member of the public. The EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from operations in 2002 are summarized here: (1) Livermore site: 0.023 mrem (0.23 {micro}Sv) (43% from point-source emissions, 57% from diffuse-source emissions). The point-source emissions include gaseous tritium modeled as tritiated water vapor as directed by EPA Region IX; the resulting dose is used for compliance purposes; and (2) Site 300: 0.021 mrem (0.21 {micro}Sv) (85% from point-source emissions, 15% from diffuse-source emissions). The EDEs were calculated using the EPA-approved CAP88-PC air dispersion/dose-assessment model, except for doses for three diffuse sources, which were calculated from measured concentrations and dose coefficients. Site specific meteorological data, stack flow data, and emissions estimates based on radionuclide usage inventory data or continuous stack monitoring data were the specific inputs to CAP88-PC for each modeled source.

  16. LLNL NESHAPs 2003 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Harrach, R J; Gallegos, G M; Peterson, S; Wilson, K R; Althouse, P E; Larson, J M; Bertoldo, N A; Tate, P J; Bowen, B

    2004-06-23

    This annual report is prepared pursuant to the National Emission Standards for Hazardous Air Pollutants (NESHAPs; Title 40 Code of Federal Regulations [CFR] Part 61, Subpart H). Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an annual effective dose equivalent (EDE) of 10 mrem (100 {micro}Sv) to any member of the public. The EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from operations in 2003 are summarized here. Livermore site: 0.044 mrem (0.44 {micro}Sv) (55% from point-source emissions, 45% from diffuse-source emissions). The point-source emissions include gaseous tritium modeled as tritiated water vapor as directed by EPA Region IX; the resulting dose is used for compliance purposes. Site 300: 0.017 mrem (0.17 {micro}Sv) (98% from point-source emissions, 2% from diffuse-source emissions). The EDEs were calculated using the EPA-approved CAP88-PC air dispersion/dose-assessment model, except for doses for two diffuse sources that were estimated using measured concentrations and dose coefficients. Site specific meteorological data, stack flow data, and emissions estimates based on radionuclide usage inventory data or continuous stack monitoring data were the specific inputs to CAP88-PC for each modeled source.

  17. LLNL NESHAPs 2002 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Harrach, R J; Gallegos, G M; Peterson, S-R; Tate, P J; Bertoldo, N A; Wilson, K R; Althouse, P E; Larson, J M

    2003-06-01

    This annual report is prepared pursuant to the National Emission Standards for Hazardous Air Pollutants (NESHAPs; Title 40 Code of Federal Regulations [CFR] Part 61, Subpart H). Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an annual effective dose equivalent (EDE) of 10 mrem (100 {micro}Sv) to any member of the public. The EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from operations in 2002 are summarized here: (1) Livermore site: 0.023 mrem (0.23 {micro}Sv) (43% from point-source emissions, 57% from diffuse-source emissions). The point-source emissions include gaseous tritium modeled as tritiated water vapor as directed by EPA Region IX; the resulting dose is used for compliance purposes; and (2) Site 300: 0.021 mrem (0.21 {micro}Sv) (85% from point-source emissions, 15% from diffuse-source emissions). The EDEs were calculated using the EPA-approved CAP88-PC air dispersion/dose-assessment model, except for doses for three diffuse sources, which were calculated from measured concentrations and dose coefficients. Site specific meteorological data, stack flow data, and emissions estimates based on radionuclide usage inventory data or continuous stack monitoring data were the specific inputs to CAP88-PC for each modeled source.

  18. LLNL NESHAPs 2003 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Harrach, R J; Gallegos, G M; Peterson, S; Wilson, K R; Althouse, P E; Larson, J M; Bertoldo, N A; Tate, P J; Bowen, B

    2004-06-23

    This annual report is prepared pursuant to the National Emission Standards for Hazardous Air Pollutants (NESHAPs; Title 40 Code of Federal Regulations [CFR] Part 61, Subpart H). Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an annual effective dose equivalent (EDE) of 10 mrem (100 {micro}Sv) to any member of the public. The EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from operations in 2003 are summarized here. Livermore site: 0.044 mrem (0.44 {micro}Sv) (55% from point-source emissions, 45% from diffuse-source emissions). The point-source emissions include gaseous tritium modeled as tritiated water vapor as directed by EPA Region IX; the resulting dose is used for compliance purposes. Site 300: 0.017 mrem (0.17 {micro}Sv) (98% from point-source emissions, 2% from diffuse-source emissions). The EDEs were calculated using the EPA-approved CAP88-PC air dispersion/dose-assessment model, except for doses for two diffuse sources that were estimated using measured concentrations and dose coefficients. Site specific meteorological data, stack flow data, and emissions estimates based on radionuclide usage inventory data or continuous stack monitoring data were the specific inputs to CAP88-PC for each modeled source.

  19. USRDS - Annual Data Report

    Data.gov (United States)

    U.S. Department of Health & Human Services — United States Renal Data System (USRDS) Annual Data Report Comprehensive statistics on chronic kidney disease and end-stage renal diseases in the United States...

  20. 2010 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    None

    2010-01-01

    This annual report includes: an overview of Western; approaches for future hydropower and transmission service; major achievements in FY 2010; FY 2010 customer Integrated Resource Planning, or IRP, survey; and financial data.

  1. Annual General Meeting

    CERN Multimedia

    Staff Association

    2014-01-01

      STAFF ASSOCIATION Our next annual general meeting will take place on : Thursday 22 May 2014 at 11:00 AM Building 40-S2-D01 For further information visit our website : https://indico.cern.ch/event/313124/

  2. Annual General Canvass Statistics

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains annual quantities and value for all seafood products that are landed and sold by established seafood dealers and brokers in the Southeast...

  3. NIRE annual report 1999

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-07-01

    The National Institute for Resources and Environment (NIRE) has a R & D concept of 'ecotechnology' that aims to protect the environment from degradation whilst promoting sustainable development. This annual report presents summaries of 32 recent research efforts.

  4. ASIST 2002 annual meeting

    CERN Multimedia

    Peek, R

    2003-01-01

    Review of discussions and presentations at the American Society for Information Science and Technology 2002 annual meeting. Topics covered included new models of scholarly publishing and the development of the semantic web (1 page).

  5. Annual Trapping Proposal 1985

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Annual Trapping Plan for the 1984-1985 trapping season at Clarence Cannon NWR outlines rules and regulations for the trapping of beaver and muskrat on the...

  6. SIS - Annual Catch Limit

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Annual Catch Limit (ACL) dataset within the Species Information System (SIS) contains information and data related to management reference points and catch data.

  7. Annual Adjustment Factors

    Data.gov (United States)

    Department of Housing and Urban Development — The Department of Housing and Urban Development establishes the rent adjustment factors - called Annual Adjustment Factors (AAFs) - on the basis of Consumer Price...

  8. Ser excepción o del individualismo.

    Directory of Open Access Journals (Sweden)

    Juan Fernando Pérez.

    1998-06-01

    Full Text Available Lección inaugural de la Maestría en Ciencias Sociales: Psicoanálisis, Cultura y Vínculo social. A partir del texto de Freud Los seres excepcionales (también traducido Las excepciones el autor interroga una categoría no psicoanalítica como es la de individualismo, con el concepto de Freud allí expuesto. Propone que el concepto freudiano ser excepción, permite pensar al menos algunas dimensiones de la fenomenología descrita bajo la categoría de individualismo, hecho de interés particular para el examen de algunas modalidades del malestar contemporáneo y de la naturaleza del vínculo social que ese mundo propone. En este orden de ideas aparece cómo las dos figuras que Freud define al lado de “las excepciones”, es decir “los delincuentes por sentimientos de culpa” y “los que fracasan al triunfar”, tienden a desdibujarse en las sociedades de hoy, en favor de una mayor significación esta forma singular de individualismo como es la de “ser excepción”. El hecho revela en forma bien específica cómo el declinamiento de padre en la época de la ciencia implica el fortalecimiento de la tendencia particular del sujeto contemporáneo de definirse como excepción frente a la ley.

  9. Timing variations in the secondary eclipse of NN Ser

    Science.gov (United States)

    Parsons, S. G.; Marsh, T. R.; Bours, M. C. P.; Littlefair, S. P.; Copperwheat, C. M.; Dhillon, V. S.; Breedt, E.; Caceres, C.; Schreiber, M. R.

    2014-02-01

    The eclipsing white dwarf plus main-sequence binary NN Serpentis provides one of the most convincing cases for the existence of circumbinary planets around evolved binaries. The exquisite timing precision provided by the deep eclipse of the white dwarf has revealed complex variations in the eclipse arrival times over the last few decades. These variations have been interpreted as the influence of two planets in orbit around the binary. Recent studies have proved that such a system is dynamically stable over the current lifetime of the binary. However, the existence of such planets is by no means proven and several alternative mechanisms have been proposed that could drive similar variations. One of these is apsidal precession, which causes the eclipse times of eccentric binaries to vary sinusoidally on many year time-scales. In this Letter, we present timing data for the secondary eclipse of NN Ser and show that they follow the same trend seen in the primary eclipse times, ruling out apsidal precession as a possible cause for the variations. This result leaves no alternatives to the planetary interpretation for the observed period variations, although we still do not consider their existence as proven. Our data limit the eccentricity of NN Ser to e detect a 3.3 ± 1.0 s delay in the arrival times of the secondary eclipses relative to the best planetary model. This delay is consistent with the expected 2.84 ± 0.04 s Rømer delay of the binary, and is the first time this effect has been detected in a white dwarf plus M dwarf system.

  10. Recubrimiento por láser de aleaciones cuasicristalinas

    Directory of Open Access Journals (Sweden)

    Audebert, F.

    1998-04-01

    Full Text Available Quasicrystals are a new class of ordenated structures with metaestable characteristics at room temperature. Quasicrystalline phases can be obtained by rapid quenching from the melt of some alloys. In general, quasicrystals present properties which make these alloys promising for wear and corrosion resistant coatings applications. During the last years, the development of quasicrystalline coatings by means of thermal spray techniques has been impulsed. However, no references have been found of their application by means of laser techniques. In this work four claddings of quasicrystalline compositions formed over aluminum substrate, produced by a continuos CO2 laser using simultaneous powders mixture injection are presented. The claddings were characterized by X ray diffraction, scanning electron microscopy and Vickers microhardness.

    Los cuasicristales constituyen un nuevo tipo de estructuras ordenadas con características metaestables a temperatura ambiente. En general, en determinadas aleaciones, pueden obtenerse por enfriamiento rápido desde el estado líquido. Han demostrado poseer buenas propiedades para su aplicación como recubrimientos resistentes al desgaste y a la corrosión. En este sentido, desde hace unos años, se ha impulsado el desarrollo de su aplicación mediante técnicas de thermal spray, aunque no se han encontrado referencias de su aplicación mediante técnicas de láser. En este trabajo, se presenta la formación de cuatro recubrimientos de aleaciones con composiciones cuasicristalinas sobre un sustrato de aluminio, utilizando un láser continuo de CO2, con inyección simultánea del material en forma de mezcla de polvos. Los mismos fueron caracterizados por difracción de rayos X, microscopía electrónica de barrido y microdureza Vickers.

  11. Natural gas annual 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-11-17

    The Natural Gas Annual provides information on the supply and disposition of natural gas to a wide audience including industry, consumers, Federal and State agencies, and educational institutions. The 1994 data are presented in a sequence that follows natural gas (including supplemental supplies) from its production to its end use. This is followed by tables summarizing natural gas supply and disposition from 1990 to 1994 for each Census Division and each State. Annual historical data are shown at the national level.

  12. Natural gas annual 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-11-01

    The Natural Gas Annual provides information on the supply and disposition of natural gas to a wide audience including industry, consumers, Federal and State agencies, and educational institutions. The 1995 data are presented in a sequence that follows natural gas (including supplemental supplies) from its production to its end use. This is followed by tables summarizing natural gas supply and disposition from 1991 to 1995 for each Census Division and each State. Annual historical data are shown at the national level.

  13. A Toolkit For CryoSat Investigations By The ESRIN EOP-SER Altimetry Team

    Science.gov (United States)

    Dinardo, Salvatore; Bruno, Lucas; Benveniste, Jerome

    2013-12-01

    The scope of this work is to feature the new tool for the exploitation of the CryoSat data, designed and developed entirely by the Altimetry Team at ESRIN EOP-SER (Earth Observation - Exploitation, Research and Development). The tool framework is composed of two separate components: the first one handles the data collection and management, the second one is the processing toolkit. The CryoSat FBR (Full Bit Rate) data is downlinked uncompressed from the satellite, containing un-averaged individual echoes. This data is made available in the Kiruna CalVal server in a 10 day rolling archive. Daily at ESRIN all the CryoSat FBR data, in SAR and SARin Mode, are downloaded (around 30 Gigabytes) catalogued and archived in local ESRIN EOP-SER workstations. As of March 2013, the total amount of FBR data is over 9 Terabytes, with CryoSat acquisition dates spanning January 2011 to February 2013 (with some gaps). This archive was built by merging partial datasets available at ESTEC and NOAA, that have been kindly made available for EOP-SER team. The on-demand access to this low level data is restricted to expert users with validated ESA P.I. credentials. Currently the main users of the archiving functionality are the team members of the Project CP4O (STSE- CryoSat Plus for Ocean), CNES and NOAA. The second component of the service is the processing toolkit. On the EOP-SER workstations there is internally and independently developed software that is able to process the FBR data in SAR/SARin mode to generate multi-looked echoes (Level 1B) and subsequently able to re-track them in SAR and SARin mode (Level 2) over open ocean, exploiting the SAMOSA model and other internally developed models. The processing segment is used for research & development scopes, supporting the development contracts awarded confronting the deliverables to ESA, on site demonstrations/training to selected users, cross- comparison against third part products (CLS/CNES CPP Products for instance), preparation

  14. Rapid on-site TLC-SERS detection of four antidiabetes drugs used as adulterants in botanical dietary supplements.

    Science.gov (United States)

    Zhu, Qingxia; Cao, Yongbing; Cao, Yingying; Chai, Yifeng; Lu, Feng

    2014-03-01

    A novel facile method has been established for rapid on-site detection of antidiabetes chemicals used to adulterate botanical dietary supplements (BDS) for diabetes. Analytes and components of pharmaceutical matrices were separated by thin-layer chromatography (TLC) then surface-enhanced Raman spectroscopy (SERS) was used for qualitative identification of trace substances on the HPTLC plate. Optimization and standardization of the experimental conditions, for example the method used for preparation of silver colloids, the mobile phase, and the concentration of colloidal silver, resulted in a very robust and highly sensitive method which enabled successful detection when the amount of adulteration was as low as 0.001 % (w/w). The method was also highly selective, enabling successful identification of some chemicals in extremely complex herbal matrices. The established TLC-SERS method was used for analysis of real BDS used to treat diabetes, and the results obtained were verified by liquid chromatography-triple quadrupole mass spectrometry (LC-MS-MS). The study showed that TLC-SERS could be used for effective separation and detection of four chemicals used to adulterate BDS, and would have good prospects for on-site qualitative screening of BDS for adulterants.

  15. Biomimetic synthesis of highly biocompatible gold nanoparticles with amino acid-dithiocarbamate as a precursor for SERS imaging

    Science.gov (United States)

    Li, Li; Liu, Jianbo; Yang, Xiaohai; Huang, Jin; He, Dinggeng; Guo, Xi; Wan, Lan; He, Xiaoxiao; Wang, Kemin

    2016-03-01

    Amino acid-dithiocarbamate (amino acid-DTC) was developed as both the reductant and ligand stabilizer for biomimetic synthesis of gold nanoparticles (AuNPs), which served as an excellent surface-enhanced Raman scattering (SERS) contrast nanoprobe for cell imaging. Glycine (Gly), glutamic acid (Glu), and histidine (His) with different isoelectric points were chosen as representative amino acid candidates to synthesize corresponding amino acid-DTC compounds through mixing with carbon disulfide (CS2), respectively. The pyrogenic decomposition of amino acid-DTC initiated the reduction synthesis of AuNPs, and the strong coordinating dithiocarbamate group of amino acid-DTC served as a stabilizer that grafted onto the surface of the AuNPs, which rendered the as-prepared nanoparticles a negative surface charge and high colloidal stability. MTT cell viability assay demonstrated that the biomimetic AuNPs possessed neglectful toxicity to the human hepatoma cell, which guaranteed them good biocompatibility for biomedical application. Meanwhile, the biomimetic AuNPs showed a strong SERS effect with an enhancement factor of 9.8 × 105 for the sensing of Rhodamine 6G, and two distinct Raman peaks located at 1363 and 1509 cm-1 could be clearly observed in the cell-imaging experiments. Therefore, biomimetic AuNPs can be explored as an excellent SERS contrast nanoprobe for biomedical imaging, and the amino acid-DTC mediated synthesis of the AuNPs has a great potential in bio-engineering and biomedical imaging applications.

  16. Synthesis of wheatear-like ZnO nanoarrays decorated with Ag nanoparticles and its improved SERS performance through hydrogenation.

    Science.gov (United States)

    Shan, Yufeng; Yang, Yong; Cao, Yanqin; Fu, Chaoli; Huang, Zhengren

    2016-04-08

    Semiconductor/noble metal composite SERS substrates have been extensively studied due to their unique bifunctionality. In this work, wheatear-like ZnO nanoarrarys have been fabricated via a modified low-temperature solution method. The hierarchical nanostructures that are constructed by stacked nanoflakes and long whiskers of ZnO possess a substantial number of characteristic nano corners and edges, which are proved to be beneficial to deposit more Ag nanoparticles (NPs). Furthermore, hydrogenated wheatear-like ZnO/AgNP composite substrates are achieved via a safe and facile solid hydrogen source (NaBH4). The hydrogenated ZnO/AgNPs (H-ZnO/Ag) substrates exhibit greatly improved SERS activity in detecting R6G molecules with an enhancement factor (EF) up to ∼0.49 × 10(8), over two orders of magnitude higher than that of the substrates before hydrogenation. The outstanding SERS performance of wheatear-like H-ZnO/Ag substrates benefits from the emerging porous structure of ZnO and abundant surface defects introduced by hydrogenation. In addition, the as-prepared substrates also show high detection sensitivity, good repeatability and recyclability, indicating great potential for practical applications.

  17. Ser-634 and Ser-636 of Kaposi’s sarcoma-associated herpesvirus RTA are involved in transactivation and are potential CDK9 phosphorylation sites

    Directory of Open Access Journals (Sweden)

    Wan-Hua eTsai

    2012-02-01

    Full Text Available The replication and transcription activator (RTA of Kaposi’s sarcoma-associated herpesvirus (KSHV, K-RTA, is a lytic switch protein that moderates the reactivation process of KSHV latency. By mass spectrometric analysis of affinity-purified K-RTA, we showed that Thr-513 or Thr-514 was the primary in vivo phosphorylation site. Thr-513 and Thr-514 are proximal to the nuclear localization signal (527KKRK530 and were previously hypothesized to be target sites of Ser/Thr kinase hKFC. However, substitutions of Thr with Ala at 513 and 514 had no effect on K-RTA subcellular localization or transactivation activity. By contrast, replacement of Ser with Ala at Ser-634 and Ser-636 located in a Ser/Pro-rich region of K-RTA, designated as S634A/S636A, produced a polypeptide with ∼10 kDa shorter in molecular weight and reduced transactivation in a luciferase reporter assay relative to the wild type. In contrast to prediction, the decrease in molecular weight was not due to lack of phosphorylation because the overall Ser and Thr phosphorylation state in K-RTA and S634A/S636A were similar, excluding that Ser-634 or Ser-636 motif served as docking sites for consecutive phosphorylation. Interestingly, S634A/S636A lost ~30% immuno-reactivity to MPM2, an antibody specific to pSer/pThr-Pro motif, indicating that 634SPSP637 motif was in vivo phosphorylated. By in vitro kinase assay, we showed that K-RTA is a substrate of CDK9, a Pro-directed Ser/Thr kinase central to transcriptional regulation. Importantly, the capability of K-RTA in associating with endogenous CDK9 was reduced in S634A/S636A, which suggested that Ser-634 and Ser-636 may be involved in CDK9 recruitment. In agreement, S634A/S636A mutant exhibited ~30% reduction in KSHV lytic cycle reactivation relative to that by the wild type K-RTA. Taken together, our data propose that Ser-634 and Ser-636 of K-RTA are phosphorylated by host transcriptional kinase CDK9 and such a process contributes to a full

  18. "Ser diferente é normal?"/"Being different: is it normal?"

    Directory of Open Access Journals (Sweden)

    Viviane Veras

    2007-01-01

    Full Text Available A pergunta título deste trabalho retoma o slogan “Ser diferente é normal”, que é parte da campanha criada para uma organização não-governamental que atende portadores de Síndrome de Down. O objetivo é a inclusão social da pessoa com deficiência e o primeiro passo foi propor a inclusão de um grupo de diferentes no grupo dito normal. No vídeo de lançamento da campanha, o diferente, identificado como normal, é mostrado por meio de exemplos – um negro com cabelo black-power, um skin-head, um corpo tatuado, um corpo feminino halterofílico, uma família hippie, uma garota com síndrome de Down. A visão da adolescente dançando reduz, de certo modo, o efeito imaginário que vai além da síndrome, uma vez que apenas o corpo com seus olhinhos puxados se destacam, e não se interrogam questões cognitivas. Minha proposta é refletir sobre o estatuto paradoxal do exemplo, tal como é trabalhado nesse vídeo: se, por definição, um exemplo mostra de fato seu pertencimento a uma classe, pode-se concluir que é exatamente por ser exemplar que ele se encontra fora dela, no exato momento em que a exibe e define. The question in the title of this paper refers to the slogan "ser diferente é normal" ("It´s normal to be different", which is part of a campaign created for a NGO that supports people with Down syndrome. The objective of the campaign is to promote the social inclusion of individuals with Down syndrome, and the first step was to propose the inclusion of a group of "differents" in the so-called normal group. The film launching the campaign shows the different identified as normal by means of examples: a black man exhibiting blackpower haircut, a skin-head, a tattooed body, an over-athletic female body, a hippie family and a girl with Down syndrome. The vision of the dancing teenager lessens the imaginary effect that surpasses the syndrome, since only her body and her little oriental eyes stand out and no cognitive issues are

  19. ¿PERMITE SER FELIZ LA MORAL CRISTIANA?

    Directory of Open Access Journals (Sweden)

    Waldo Romo P

    2006-01-01

    Full Text Available La pregunta que encabeza el artículo ya es desafiante: "¿Permite ser feliz la moral cristiana?". Existe la posibilidad de responder negativamente. El autor, después de analizar algunos pensadores (Nietzsche, entre ellos que han negado a la moral cristiana la posibilidad de hacer feliz al ser humano, presenta las condiciones de posibilidad para que dicha moral se encauce a ser un camino de felicidad. Entre esas condiciones se destaca: el paso de una moral heterónama a una moral de autonomía teónoma, una moral centrada en la persona más que una moral centrada en la ley, una moral de discipulado que pone primero el amor y luego el cumplimiento normativo. Profundiza, al respecto, en el logion de Jesucristo: "El que me ama, cumple mis mandamientos". Esta enseñanza plantea el orden secuencial de toda moral cristiana: porque se ama a Jesús, se entiende el cumplimiento de las normas. Estas se transforman, entonces, en el "test" verificador del amor. De lo contrario, se permanece en un ritualismo normativo, que en defintiva hastía y no lleva a la felicidad. Por ello, el artículo profundiza en una moral de opción fundamental expresada en actitudes y actos morales coherentes con esa opción. Si las condiciones de posibilidad propuestas se llegan a dar, la interrogante que organiza el artículo tendrá una respuesta positivaThe question that heads this article is already challenging: "Does Christian morality permit one to be happy?" The possibility of responding negatively exists. The author, after analyzing the contributions of some thinkers (Nietszche among them who have denied the possibility that Christian morality could make human beings happy, presents the conditions that would orient said morality towards the possibility of becoming a path to happiness. Among those conditions, the author highlights: the passage from a "heteronomous" morality to a morality of "theonomous" autonomy, a morality centered on the person rather than a morality

  20. Surface modification of SERS substrates with plasma-polymerized trimethylsilane nanocoating

    Science.gov (United States)

    Kim, Young Jo; Sun, Xin; Jones, John E.; Lin, Mengshi; Yu, Qingsong; Li, Hao

    2015-03-01

    Surface-enhanced Raman scattering (SERS) substrates were modified by depositing a nanometer-thick polymer coating on top of SERS-active surface. This thin hydrophobic nanocoating, achieved by low temperature plasma polymerization of trimethylsilane, was found to reduce surface energy of SERS substrate and in turn help relieve the analyte spreading on the surface of SERS substrates. Detection of melamine molecules with these surface-modified SERS substrates showed that this plasma nanocoating improved, not significantly though, SERS sensitivity in comparison with unmodified SERS substrates. It is believed that the increased hydrophobicity induced by this plasma nanocoating had two folds of beneficial effects on SERS sensitivity. First, the as-produced hydrophobic surface gave rise to preconcentration effect due to the reduced contact area between analyte molecules and the substrate surface. Second, the decreased surface energy of SERS substrates was helpful in placing analyte molecules in SERS hot spots. These two combined gains were deemed to outweigh the loss of SERS sensitivity caused by enlarged distance between metal surface and analyte molecules.

  1. Balthasar y la metafísica del "ser donado"

    Directory of Open Access Journals (Sweden)

    Carlos Casale

    2009-01-01

    Full Text Available En este artículo se desarrolla la influencia táctica de Heidegger en Hans Urs von Balthasar, especialmente en "La acción". La influencia se ve en dos niveles. Primero de la obra "Ser y tiempo" en la comprensión balthasariana de la temporalidad y finitud, como característica básica -y olvidada a veces- del hombre, que sólo así puede estar abierto a la revelación. El segundo nivel es la obra "Nietzsche" del filósofo de Friburgo. Es sobre todo esta última obra la que le ayuda a Balthasar a pensar a Dios como "acontecimiento", evento gratuito plenificador del hombre. Hay así al interior de la dramática una cierta "analogía" entre la crítica "al olvido del ser" y al "olvido del reconocerse donado", actitud básica del hombre, según Balthasar, frente a la iniciativa de Dios de regalarse, en alteridad, para salvarlo en su temporalidad e historia.In this article, an examination of the tactical influence of Heidegger upon Hans Urs von Balthasar is developed, especially in the theo-drama The Action. This influence is seen on two levels. First, in the work Being and Time on the Balthasarian understanding of temporality and finitude as a basic characteristic -sometimes forgotten- of the human person, who only in this manner can be open to revelation. The second level is the work Nietzsche by the Freiburg philosopher. It is above all this latter work that helps Balthasar to think of God as "event", a gracious fulfilling occurrence of the human person. Thus there exists in the interior of the dramatic a certain "analogy" between the critical "forgetting of being" and the "forgetting of recognizing oneself as given", a basic attitude in the human person, according to Balthasar, when faced with God's initiative of giving Himself as gift, in alterity, in order to save him in his temporality and history.

  2. Nucleotide sequences of three tRNA(Ser) from Drosophila melanogaster reading the six serine codons.

    Science.gov (United States)

    Cribbs, D L; Gillam, I C; Tener, G M

    1987-10-05

    The nucleotide sequences of three serine tRNAs from Drosophila melanogaster, together capable of decoding the six serine codons, were determined. tRNA(Ser)2b has the anticodon GCU, tRNA(Ser)4 has CGA and tRNA(Ser)7 has IGA. tRNA(Ser)2b differs from the last two by about 25%. However, tRNA(Ser)4 and tRNA(Ser)7 are 96% homologous, differing only at the first position of the anticodon and two other sites. This unusual sequence relationship suggests, together with similar pairs in the yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae, that eukaryotic tRNA(Ser)UCN may be undergoing concerted evolution.

  3. Aggregation induced Raman scattering of squaraine dye: Implementation in diagnosis of cervical cancer dysplasia by SERS imaging.

    Science.gov (United States)

    Narayanan, Nisha; Karunakaran, Varsha; Paul, Willi; Venugopal, Karunakaran; Sujathan, K; Kumar Maiti, Kaustabh

    2015-08-15

    The extent of squaraine dye aggregation that reflects on surface enhanced Raman signal scattering (SERS) intensity upon adsorption on nano-roughened gold surface has been investigated. Here we have synthesized a serious of six squaraine dyes consisting of two different electron donor moiety i.e. 1,1,2-trimethyl-1H-benzo[e]indole and 2-methylbenzo[d]thiazole which modulates the chemisorptions and hydrophobicity being designated as SQ1, SQ2, SQ3, SQ4, SQ5 and SQ6. Interestingly, SQ2 (mono lipoic acid appended), SQ5 and SQ6 (conjugated with hexyl and dodecyl side chain) squaraine derivatives having more tendency of aggregation in DMSO-water mixed solvent showed significant increase of Raman scattering in the fingerprint region when chemisorbed on spherical gold nanoparticles. Two sets of SERS nanotags were prepared with colloidal gold nanoparticle (Au-NPs size: 40 nm) by incorporating Raman reporters SQ2 and SQ5 followed by thiolated PEG encapsulation (SH-PEG, SH-PEG-COOH) denoted as AuNPs-SQ2-PEG and AuNPs-SQ5-PEG. Further conjugation of these nanotag with monoclonal antibodies specific to over expressed receptors, EGFR and p16/Ki-67 in cervical cancer cell, HeLa showed prominent SERS mapping intensity and selectivity towards cell surface and nucleus. The fast and accurate recognition obtained by antibody triggered SERS-nanotag has been compared with conventional time consuming immunocytochemistry technique which prompted us to extend further investigation using real patient cervical smear sample for a non-invasive, ultrafast and accurate diagnosis.

  4. 半核壳 PS@Ag/Fe/Ag 阵列的制备与 SERS 性能研究%Semi-nanoshell of PS@Ag/Fe/Ag Array for SERS Substrate

    Institute of Scientific and Technical Information of China (English)

    华中; 孙欢欢; 张梦宁; 刘毛毛; 陈雷; 韩无双; 张永军

    2016-01-01

    表面增强拉曼散射( SERS)光谱已广泛应用在生命科学、化学、医学等诸多领域.设计了一种二维有序的半核壳SERS活性基底.利用自组装技术制备聚苯乙烯( PS)球核,通过磁控溅射系统在其表面包覆Ag/Fe/Ag三层壳,改变Fe的溅射时间,制成不同厚度铁磁性层的半核壳PS@Ag/Fe/Ag阵列.采用532 nm激发波长,选择对巯基苯胺作为探针分子研究SERS基底的活性,其增强因子可达到107.SERS的增强主要来源于物理增强(即电磁场增强),实现基底的高灵敏度检测和重现性.铁磁性层的引入对PS@Ag/Fe/Ag阵列的SERS增强具有较好的调控作用.%Surface-enhanced Raman scattering ( SERS ) spectroscopy has been widely used in life science , chemistry ,medicine and many other areas .In this paper , we presented a two-dimensional ordered polystyrene ( PS) microsphere array by self-assembly technology and used magnetron sputtering system to prepare semi-nanoshell array of Ag/Fe/Ag on the PS array .By changing sputtering time of magnetic layer Fe ,we prepared the different thicknesses of core-shell SERS active substrates .We applied an excitation wavelength of 532 nm and used P-aminothiophenol as a probe molecule to study SERS property and enhancement factor can reach 107 .

  5. 2008 Annual Report TATRC

    Science.gov (United States)

    2008-01-01

    Catheters Trauma to the limbs , or extremities, has always been common among injured Warfighters. The closely associated Compartment Syndrome (CS), in...Mirror-box and Mental Visualization Treatments on Phantom Limb Pain (PLP) CDR Jack Tsao, Department of Neurology, Uniformed Ser- vices University of...therapy has been shown to reduce phantom limb pain. The goal of this research is to develop an objec- tive measure of the response of PLP to mirror

  6. Synthesis of Au NP@MoS2 Quantum Dots Core@Shell Nanocomposites for SERS Bio-Analysis and Label-Free Bio-Imaging

    Directory of Open Access Journals (Sweden)

    Xixi Fei

    2017-06-01

    Full Text Available In this work, we report a facile method using MoS2 quantum dots (QDs as reducers to directly react with HAuCl4 for the synthesis of Au nanoparticle@MoS2 quantum dots (Au NP@MoS2 QDs core@shell nanocomposites with an ultrathin shell of ca. 1 nm. The prepared Au NP@MoS2 QDs reveal high surface enhanced Raman scattering (SERS performance regarding sensitivity as well as the satisfactory SERS reproducibility and stability. The limit of detection of the hybrids for crystal violet can reach 0.5 nM with a reasonable linear response range from 0.5 μM to 0.5 nM (R2 ≈ 0.974. Furthermore, the near-infrared SERS detection based on Au NP@MoS2 QDs in living cells is achieved with distinct Raman signals which are clearly assigned to the various cellular components. Meanwhile, the distinguishable SERS images are acquired from the 4T1 cells with the incubation of Au NP@MoS2 QDs. Consequently, the straightforward strategy of using Au NP@MoS2 QDs exhibits great potential as a superior SERS substrate for chemical and biological detection as well as bio-imaging.

  7. Fabrication of cube-like Fe3O4@SiO2@Ag nanocomposites with high SERS activity and their application in pesticide detection

    Science.gov (United States)

    Li, Lei; Zhao, Aiwu; Wang, Dapeng; Guo, Hongyan; Sun, Henghui; He, Qinye

    2016-07-01

    The cube-like Fe3O4@SiO2@Ag (FSA) nanocomposites with great SERS activity have been successfully synthesized by a layer-by-layer procedure in this paper. The cube-like Fe3O4@SiO2 core-shell structures were prepared via a new route and Ag nanoparticles were introduced onto their surface through a one-pot hydrothermal reaction. By controlling the reaction time, the coverage rate of Ag on the FSA surface could be tuned, and then a series of FSA composites were obtained. The SERS properties of these FSA composites were investigated using p-aminothiophenol ( p-ATP) as the probe molecule. It was found that the FSA composites synthesized with a reaction time of 6 h showed the best SERS performance, and the detection limit for p-ATP could reach 1 × 10-7 M. For practical application, the FSA composites were also used to detect thiram, one of the dithiocarbamate fungicides that has been widely used as a pesticide in agriculture. The detection limit is as low as 1 × 10-6 M (0.24 ppm), lower than the maximal residue limit of 7 ppm in fruit prescribed by the US Environmental Protection Agency. The resulting substrate with high SERS activity, stability and strong magnetic responsivity makes the FSA composite a perfect choice for practical SERS detection applications.

  8. Orientation of N-(1-(2-chlorophenyl)-2-(2-nitrophenyl)ethyl)-4-methylbenzenesulfonamide on silver nanoparticles: SERS studies.

    Science.gov (United States)

    Anuratha, M; Jawahar, A; Umadevi, M; Sathe, V G; Vanelle, P; Terme, T; Meenakumari, V; Milton Franklin Benial, A

    2014-10-15

    In the present study, the silver nanoparticles were synthesized using a solution combustion method with urea as fuel. The prepared silver nanoparticles show an FCC crystalline structure with particle size of 59nm. FESEM image shows the prepared silver is a rod like structure. The surface-enhanced Raman scattering (SERS) spectrum indicates that the N-(1-(2-chlorophenyl)-2-(2-nitrophenyl)ethyl)-4-methylbenzenesulfonamide (CS) molecule adsorbed on the silver nanoparticles. The spectral analysis reveals that the sulfonamide is adsorbed by tilted orientation on the silver surface. The Hatree Fock calculations were also performed to predict the vibrational motions of CS. This present investigation has been a model system to deduce the interaction of drugs with DNA. Copyright © 2014 Elsevier B.V. All rights reserved.

  9. High Surface-Enhanced Raman Scattering (SERS) Amplification Factor Obtained with Silver Printed Circuit Boards and the Influence of Phenolic Resins for the Characterization of the Pesticide Thiram.

    Science.gov (United States)

    Silva de Almeida, Francylaine; Bussler, Larissa; Marcio Lima, Sandro; Fiorucci, Antonio Rogério; da Cunha Andrade, Luis Humberto

    2016-07-01

    In this work, low-cost substrates with rough silver surfaces were prepared from commercial copper foil-covered phenolic board (CPB) and an aqueous solution of AgNO3, and were used for surface-enhanced Raman scattering (SERS) and surface-enhanced resonance Raman scattering (SERRS) measurements. A maximum SERS amplification factor of 1.2 × 10(7) was obtained for Rhodamine 6G (R6G), and use of the CPB resulted in a detection limit for Thiram pesticide of 0.5 µmol L(-1) The minimum detection level was limited by residual traces of phenolic groups that originated from the substrate resin, which became solubilized in the aqueous Ag(+) solution. It was found that the bands corresponding to the impurities had less influence in the Thiram analysis, which could be explained by the high affinity of sulfur for Ag surfaces. The influence of impurities in the SERS analyses therefore depended on the linkage between the rough silver surface and the analyte. The findings demonstrated the ease and effectiveness of using CPB to prepare a nanostructured surface for SERS. © The Author(s) 2016.

  10. Timing variations in the secondary eclipse of NN Ser

    CERN Document Server

    Parsons, S G; Bours, M C P; Littlefair, S P; Copperwheat, C M; Dhillon, V S; Breedt, E; Caceres, C; Schreiber, M R

    2013-01-01

    The eclipsing white dwarf plus main-sequence binary NN Serpentis provides one of the most convincing cases for the existence of circumbinary planets around evolved binaries. The exquisite timing precision provided by the deep eclipse of the white dwarf has revealed complex variations in the eclipse arrival times over the last few decades. These variations have been interpreted as the influence of two planets in orbit around the binary. Recent studies have proved that such a system is dynamically stable over the current lifetime of the binary. However, the existence of such planets is by no means proven and several alternative mechanisms have been proposed that could drive similar variations. One of these is apsidal precession, which causes the eclipse times of eccentric binaries to vary sinusoidally on many year timescales. In this paper we present timing data for the secondary eclipse of NN Ser and show that they follow the same trend seen in the primary eclipse times, ruling out apsidal precession as a poss...

  11. SERS of semiconducting nanoparticles (TIO{sub 2} hybrid composites).

    Energy Technology Data Exchange (ETDEWEB)

    Rajh, T.; Musumeci, A.; Gosztola, D.; Schiller, T.; Dimitrijevic, N. M.; Mujica, V.; Martin, D.; Center for Nanoscale Materials

    2009-05-06

    Raman scattering of molecules adsorbed on the surface of TiO{sub 2} nanoparticles was investigated. We find strong enhancement of Raman scattering in hybrid composites that exhibit charge transfer absorption with TiO{sub 2} nanoparticles. An enhancement factor up to {approx}10{sup 3} was observed in the solutions containing TiO{sub 2} nanoparticles and biomolecules, including the important class of neurotransmitters such as dopamine and dopac (3,4-dihydroxy-phenylacetic acid). Only selected vibrations are enhanced, indicating molecular specificity due to distinct binding and orientation of the biomolecules coupled to the TiO{sub 2} surface. All enhanced modes are associated with the asymmetric vibrations of attached molecules that lower the symmetry of the charge transfer complex. The intensity and the energy of selected vibrations are dependent on the size and shape of nanoparticle support. Moreover, we show that localization of the charge in quantized nanoparticles (2 nm), demonstrated as the blue shift of particle absorption, diminishes SERS enhancement. Importantly, the smallest concentration of adsorbed molecules shows the largest Raman enhancements suggesting the possibility for high sensitivity of this system in the detection of biomolecules that form a charge transfer complex with metal oxide nanoparticles. The wavelength-dependent properties of a hybrid composite suggest a Raman resonant state. Adsorbed molecules that do not show a charge transfer complex show weak enhancements probably due to the dielectric cavity effect.

  12. Peculiarities of the SEHRS and SERS Spectra of - Bipyridine Molecule

    CERN Document Server

    Chelibanov, Vladimir P; Polubotko, Aleksey M

    2016-01-01

    The SEHRS and SERS spactra of 4,4 - Bipyridine are analyzed on the base of the Dipole-Quadrupole theory for two possible geometries of the molecule. It is demonstrated that there appear strong lines caused by vibrations transforming after a unit irreducible representation both for the geometry with D2 and D2h symmetry groups, which may probably describe the symmetry properties of the molecule. Appearance of these lines is associated with a strong quadrupole light-molecule interaction, which arises in nano size rregions of sharp roughness of the metal. In addition, there are the lines caused by contributions from both the vibrations transforming after the unit irredicible representations A or Ag and the representations B1 or B1u, respectively, which describe transformational properties of the Ez component of the dipole moment, which is perpendicular to the surface for both geometries. This result is associated with a specific geometry of the molecule, when the indicated vibrations can be nearly degenerated and...

  13. Fotografia: viajar, ver e ser visto na internet

    Directory of Open Access Journals (Sweden)

    Anabel S. Cavalcanti

    2011-02-01

    Full Text Available

    Este artigo pretende tratar da fotografia – especialmente turística – como meio de ver e ser visto, numa visão antropológica das Teorias da Imagem. O valor dado às memórias e à identidade familiar nos tradicionais álbuns de família é transferido à exposição virtual na rede mundial de computadores, facilitada pelas novas tecnologias. Mais do que aproveitar as viagens turísticas, na contemporaneidade se quer mostrar que viajou. E para isso não existe melhor caminho do que fotografar-se e exibir-se na internet.


  14. Flower bouquet variation in four species of Crocus ser. Verni.

    Science.gov (United States)

    Carta, Angelino; Flamini, Guido; Cioni, Pier Luigi; Pistelli, Luisa; Peruzzi, Lorenzo

    2015-01-01

    Flowering plants employ a wide variety of signals, including scent, to attract pollinators. The aim of this work was to examine whether flower volatiles in four closely related Crocus species are linked to species divergence and to the current knowledge on their pollination syndromes. Fragrances of freshly opened flowers in Crocus etruscus, C. ilvensis, C. neglectus, and C. vernus, all belonging to ser. Verni, were analyzed using GC/MS. Results coincide with present knowledge about systematic relationships among taxa. The four species fall into two main fragrance types, based on similarities of their volatile compounds. In C. etruscus, C. ilvensis, and C. neglectus, oxygenated monoterpenes (lilac aldehyde B and A) are most abundant, while C. vernus has a fragrance rich in monoterpene hydrocarbons (α-pinene and limonene). Our results point towards outcrossing mating strategies for C. etruscus, C. ilvensis, and C. neglectus, whose volatile compounds are known as pollinator attractants. This is in line with their flower architecture, showing a style of variable height, often overtopping stamens. On the other hand, a self-pollination strategy was repeatedly suggested in the literature for C. vernus, marked by flowers with the style deeply inserted in the stamens and also by a completely different flower bouquet.

  15. SDS bubbles functionalized with Gold nanoparticles and SERS applications

    Science.gov (United States)

    Navarro-Badilla, A.; Hurtado, R. Britto; Cortez-Valadez, M.; Perez-Rodriguez, A.; Flores-Acosta, M.; Maldonado-Arce, A.

    2017-03-01

    We present a method of incorporation of gold nanoparticles in SDS (sodium dodecyl sulfate) bubbles with a low polydispersity index (monodispersed nanoparticles). Both the bubbles and nanoparticles maintained their structural and morphologic properties after functionalization. The bubbles present a radio of 0.38 mm with a standard deviation of±0.018 mm. The gold nanoparticles were obtained with sucrose as the catalytic agent and ascorbic acid as the reducing agent. The nanoparticles display several geometric morphologies as well as sizes inferior to 50 nm, as observed in the images obtained with Transmission Electron Microscopy (TEM). The optical properties were studied by optical absorption spectroscopy. The absorption band linked to the surface plasmon resonance (SPR) is located at 550 nm before and after the functionalization of the bubbles. Moreover, microscopic bubbles with a diameter smaller than 1 μm with the ability to stabilize nanoparticles in their surface were found in isolated regions of the sample. Additionally, the Surface Enhancement Raman Spectroscopy (SERS) properties of the colloid were analyzed with common drugs.

  16. Juan O’Gorman. Formas de no ser arquitecto

    Directory of Open Access Journals (Sweden)

    Javier Jerez González

    2015-12-01

    Full Text Available La obra proyectada y construida por Juan O’Gorman a lo largo de su vida traza un recorrido largo y sincopado entre formas de entender la arquitectura totalmente antagónicas. Su primera etapa de radical racionalismo desarrolla, según procedimientos estrictamente técnicos, un lenguaje revolucionario basado en la austeridad y la precisión. A pesar de ello muchas de sus obras destacan por la plasticidad de sus composiciones volumétricas, la riqueza espacial o, incluso, por la creación de atmósferas oníricas. Los primeros años de frenética actividad dan paso a un periodo de voluntario alejamiento de la práctica profesional de la arquitectura: ni una sola obra, ni un solo proyecto durante cerca de 15 años. Tan solo su actividad docente le mantiene en contacto con el mundo de la arquitectura. Cuando Juan O’Gorman vuelve a ejercer como arquitecto, lo hace construyendo algunos de los iconos del movimiento de integración plástica de México: la biblioteca central de la UNAM y su casa en San Jerónimo. A pesar de las enormes diferencias, o más bien oposiciones, entre unos momentos y otros, puede identificarse una invariante clara como señal de identidad personal: una velada voluntad de no ser arquitecto

  17. Em Busca de um Ser Humano Não Programado

    Directory of Open Access Journals (Sweden)

    John Schranz

    2014-05-01

    Full Text Available Stanislavski diz a seus atores: o Estúdio é para a Vida. Meyerhold acrescenta: o Teatro é a arte do Homem. Craig prevê um novo ator: Hoje ele personifica e interpreta; amanhã ele precisará representar e interpretar, e no terceiro dia ele irá criar. Grotowski e Barba aproveitam as forças do teatro oriental na epifania do Performer. Finalmente suprimindo a dependência do autor para criar a partir de si mesmo, o novo Performer obtém autonomia artística. Além disso: ao contrário de outros artistas, ele cria através de si mesmo, sem o uso de um meio. O papel do Diretor pressupõe uma fase intermediária: sem a base estável e unificadora do texto do Autor a autonomia arduamente adquirida pelo Performer poderia degenerar-se em individualismo centrado no ego. Segundo a neurociência, a ação precede a consciência. Fora de seu ato criativo como artista e dentro dele como matéria-prima, o Performer desafia limites. Ele é um sujeito-objeto, uma busca pelo Ser Humano não programado.

  18. International energy annual 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-02-01

    The International Energy Annual presents an overview of key international energy trends for production, consumption, imports, and exports of primary energy commodities in over 220 countries, dependencies, and areas of special sovereignty. Also included are population and gross domestic product data, as well as prices for crude oil and petroleum products in selected countries. Renewable energy reported in the International Energy Annual includes hydroelectric power, geothermal, solar, and wind electric power, biofuels energy for the US, and biofuels electric power for Brazil. New in the 1996 edition are estimates of carbon dioxide emissions from the consumption of petroleum and coal, and the consumption and flaring of natural gas. 72 tabs.

  19. Natural gas annual 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-10-01

    The Natural Gas Annual provides information on the supply and disposition of natural gas to a wide audience including industry, consumers, Federal and State agencies, and educational institutions. The 1997 data are presented in a sequence that follows natural gas (including supplemental supplies) from its production to its end use. This is followed by tables summarizing natural gas supply and disposition from 1993 to 1997 for each Census Division and each State. Annual historical data are shown at the national level. 27 figs., 109 tabs.

  20. Fasting mediated increase in p-BAD(ser155) and p-AKT(ser473) in the prefrontal cortex of mice.

    Science.gov (United States)

    Pitchaimani, Vigneshwaran; Arumugam, Somasundaram; Thandavarayan, Rajarajan Amirthalingam; Karuppagounder, Vengadeshprabhu; Sreedhar, Remya; Afrin, Rejina; Harima, Meilei; Suzuki, Hiroshi; Miyashita, Shizuka; Nomoto, Mayumi; Sone, Hirohito; Suzuki, Kenji; Watanabe, Kenichi

    2014-09-05

    BAD-deficient mice and fasting have several common functional roles in seizures, beta-hydroxybutyrate (BHB) uptake in brain and alteration in counterregulatory hormonal regulation during hypoglycemia. Neuronal specific insulin receptor knockout (NIRKO) mice display impaired counterregulatory hormonal responses during hypoglycemia. In this study we investigated the fasting mediated expression of p-BAD(ser155) and p-AKT(ser473) in different regions of brain (prefrontal cortex, hippocampus, midbrain and hypothalamus). Fasting specifically increases p-BAD(ser155) and p-AKT(ser473) in prefrontal cortex and decreases in other regions of brain. Our results suggest that fasting may increase the uptake BHB by decreasing p-BAD(ser155) in the brain during hypoglycemia except prefrontal cortex and it uncovers specific functional area of p-BAD(ser155) and p-AKT(ser473) that may regulates counter regulatory hormonal response. Overall in support with previous findings, fasting mediated hypoglycemia activates prefrontal cortex insulin signaling which influences the hypothalamic paraventricular nucleus mediated activation of sympathoadrenal hormonal responses.

  1. Industrial Applications of the Surface-Enhanced Raman Spectroscopy Application industrielle du SERS

    Directory of Open Access Journals (Sweden)

    Nabiev I.

    2006-11-01

    Full Text Available Surface-enhanced Raman scattering (SERS spectroscopy is now a well-established phenomenon, which has been thoroughly characterized in a variety of interfacial and colloidal environments. Although some quantitative aspects of the underlying enhancement mechanisms apparently remain unresolved, attention is now shifting towards application of SERS to explore phenomena of chemical, physical, biological and industrial significance. The goal of this review is to appreciate the industrial value of innovative SERS technique on the basis of our experience in development of new SERS-active substrates and in their biomedical and biotechnological applications. Examples of diverse SERS analytical applications as well as some very recent facilities, as SERS microprobe analysis, SERS fiber optics probes, FT-SERS spectroscopy, SERS detection for high-performance liquid chromatography, etc. , are also discussed. Le SERS (Surface Enhanced Raman Spectroscopy est un phénomène aujourd'hui bien connu qui a été étudié dans toute une gamme de milieux interfaciaux et colloïdaux. Si certains aspects quantitatifs des mécanismes d'exaltation restent apparemment non résolus, l'attention se porte à présent vers l'application de la spectroscopie SERS à l'exploration de phénomènes présentant un intérêt chimique, physique, biologique et industriel. L'objectif de cet article est d'estimer la valeur industrielle des nouvelles techniques de spectroscopie SERS à partir de notre expérience dans le développement de nouveaux substrats actifs en SERS et leurs applications biomédicales et biotechnologiques. Les auteurs discutent également des exemples de diverses applications analytiques de la spectroscopie SERS ainsi que de quelques procédés très récents : analyse par microsonde SERS, sondes SERS à fibres optiques, spectroscopie FT-SERS, détection SERS pour la chromatographie haute performance en phase liquide, etc.

  2. Engineering Annual Summary 1998

    Energy Technology Data Exchange (ETDEWEB)

    Dimolitsas, S

    1999-05-01

    Unlike most research and development laboratories, Lawrence Livermore National Laboratory (LLNL) is responsible for delivering production-ready designs. Unlike most industry, LLNL is responsible for R and D that must significantly increase the nation's security. This rare combination of production engineering expertise and national R and D agenda identifies LLNL as one of the few organizations today that conducts cutting-edge engineering on grand-scale problems, while facing enormous technical risk and undergoing diligent scrutiny of its budget, schedule, and performance. On the grand scale, cutting-edge technologies are emerging from our recent ventures into ''Xtreme Engineering{trademark}.'' Basically, we must integrate and extend technologies concurrently and then push them to their extreme, such as building very large structures but aligning them with extreme precision. As we extend these technologies, we push the boundaries of engineering capabilities at both poles: microscale and ultrascale. Today, in the ultrascale realm, we are building NIF, the world's largest laser, which demands one of the world's most complex operating systems with 9000 motors integrated through over 500 computers to control 60,000 points for every laser shot. On the other pole, we have fabricated the world's smallest surgical tools and the smallest instruments for detecting biological and chemical agents used by antiterrorists. Later in this Annual Summary, we highlight some of our recent innovations in the area of Xtreme Engineering, including large-scale computer simulations of massive structures such as major bridges to prepare retrofitting designs to withstand earthquakes. Another feature is our conceptual breakthrough in developing the world's fastest airplane, HyperSoar, which can reach anywhere in the planet in two hours at speeds of 6700 mph. In the last few years, Engineering has significantly pushed the technology in structural

  3. Loss of p53 Ser18 and Atm results in embryonic lethality without cooperation in tumorigenesis.

    Directory of Open Access Journals (Sweden)

    Heather L Armata

    Full Text Available Phosphorylation at murine Serine 18 (human Serine 15 is a critical regulatory process for the tumor suppressor function of p53. p53Ser18 residue is a substrate for ataxia-telangiectasia mutated (ATM and ATM-related (ATR protein kinases. Studies of mice with a germ-line mutation that replaces Ser18 with Ala (p53(S18A mice have demonstrated that loss of phosphorylation of p53Ser18 leads to the development of tumors, including lymphomas, fibrosarcomas, leukemia and leiomyosarcomas. The predominant lymphoma is B-cell lymphoma, which is in contrast to the lymphomas observed in Atm(-/- animals. This observation and the fact that multiple kinases phosphorylate p53Ser18 suggest Atm-independent tumor suppressive functions of p53Ser18. Therefore, in order to examine p53Ser18 function in relationship to ATM, we analyzed the lifespan and tumorigenesis of mice with combined mutations in p53Ser18 and Atm. Surprisingly, we observed no cooperation in survival and tumorigenesis in compound p53(S18A and Atm(-/- animals. However, we observed embryonic lethality in the compound mutant animals. In addition, the homozygous p53Ser18 mutant allele impacted the weight of Atm(-/- animals. These studies examine the genetic interaction of p53Ser18 and Atm in vivo. Furthermore, these studies demonstrate a role of p53Ser18 in regulating embryonic survival and motor coordination.

  4. Role of Ser7 phosphorylation of the CTD during transcription of snRNA genes

    Science.gov (United States)

    Egloff, Sylvain

    2012-01-01

    The largest subunit of RNA polymerase (pol) II, Rpb1, contains an unusual carboxyl-terminal domain (CTD) composed of consecutive repeats of the sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser (Y1S2P3T4S5P6S7). During transcription, Ser2, Ser5 and Ser7 are subjected to dynamic phosphorylation and dephosphorylation by CTD kinases and phosphatases, creating a characteristic CTD phosphorylation pattern along genes. This CTD “code” allows the coupling of transcription with co-transcriptional RNA processing, through the timely recruitment of the appropriate factors at the right point of the transcription cycle. In mammals, phosphorylation of Ser7 (Ser7P) is detected on all pol II-transcribed genes, but is only essential for expression of a sub-class of genes encoding small nuclear (sn)RNAs. The molecular mechanisms by which Ser7P influences expression of these particular genes are becoming clearer. Here, I discuss our recent findings clarifying how Ser7P facilitates transcription of these genes and 3′end processing of the transcripts, through recruitment of the RPAP2 phosphatase and the snRNA gene-specific Integrator complex. PMID:22858677

  5. The IkappaB kinase family phosphorylates the Parkinson's disease kinase LRRK2 at Ser935 and Ser910 during Toll-like receptor signaling.

    Directory of Open Access Journals (Sweden)

    Nicolas Dzamko

    Full Text Available Mutations in leucine-rich repeat kinase 2 (LRRK2 are strongly associated with late-onset autosomal dominant Parkinson's disease. LRRK2 is highly expressed in immune cells and recent work points towards a link between LRRK2 and innate immunity. Here we demonstrate that stimulation of the Toll-Like Receptor (TLR pathway by MyD88-dependent agonists in bone marrow-derived macrophages (BMDMs or RAW264.7 macrophages induces marked phosphorylation of LRRK2 at Ser910 and Ser935, the phosphorylation sites that regulate the binding of 14-3-3 to LRRK2. Phosphorylation of these residues is prevented by knock-out of MyD88 in BMDMs, but not the alternative TLR adaptor protein TRIF. Utilising both pharmacological inhibitors, including a new TAK1 inhibitor, NG25, and genetic models, we provide evidence that both the canonical (IKKα and IKKβ and IKK-related (IKKε and TBK1 kinases mediate TLR agonist induced phosphorylation of LRRK2 in vivo. Moreover, all four IKK members directly phosphorylate LRRK2 at Ser910 and Ser935 in vitro. Consistent with previous work describing Ser910 and Ser935 as pharmacodynamic biomarkers of LRRK2 activity, we find that the TLR independent basal phosphorylation of LRRK2 at Ser910 and Ser935 is abolished following treatment of macrophages with LRRK2 kinase inhibitors. However, the increased phosphorylation of Ser910 and Ser935 induced by activation of the MyD88 pathway is insensitive to LRRK2 kinase inhibitors. Finally, employing LRRK2-deficient BMDMs, we present data indicating that LRRK2 does not play a major role in regulating the secretion of inflammatory cytokines induced by activation of the MyD88 pathway. Our findings provide the first direct link between LRRK2 and the IKKs that mediate many immune responses. Further work is required to uncover the physiological roles that phosphorylation of LRRK2 by IKKs play in controlling macrophage biology and to determine how phosphorylation of LRRK2 by IKKs impacts upon the use of Ser

  6. SER Y ACCIÓN SOCIAL EN EL HORIZONTE DE LA REFLEXIÓN ACERCA DEL TIEMPO DESARROLLADA POR HEIDEGGER EN SER Y TIEMPO

    OpenAIRE

    Domingo Fernández Agis

    2014-01-01

    Según plantea Heidegger en Ser y tiempo, la pregunta por el ser no es fruto de la espontaneidad de la llamada conciencia natural, aunque tampoco en la racionalidad científica pueda encontrar los elementos adecuados para su formulación. Sabemos, en todo caso, que la ciencia puede contribuir a la respuesta, aunque no ayude a comprender lo que el hecho mismo de esa enunciación implica. La tesis que se defiende en este trabajo es que la correlación entre la pregunta por el ser y la pregunta por e...

  7. Ser e não ser gente: dinâmicas da feitiçaria no alto Xingu

    Directory of Open Access Journals (Sweden)

    Marina Vanzolini

    2013-08-01

    Full Text Available Baseado em uma pesquisa de campo com os Aweti, grupo tupi do alto Xingu, o artigo propõe uma reflexão sobre as particularidades da agressão mágica xinguana no cenário amazônico, relacionando técnicas, campos de incidência e efeitos políticos de tais práticas. Contrariando uma imagem corrente na etnologia regional, os xinguanos sempre identificam a fonte de seus maiores sofrimentos com a ação de pessoas bastante próximas, frequentemente coaldeãos da vítima, e sempre outros xinguanos, isto é, integrantes do que Ellen Basso denominou de "comunidade moral" xinguana: um conjunto multiétnico de aliados que se define, idealmente, pela abdicação da violência entre si. A hipótese central deste trabalho é que a feitiçaria xinguana deveria ser pensada como parte de um mecanismo de produção de inimizade que, operando a partir das relações cotidianas, faz com que o esforço histórico de produção de identidade comunitária pareça nunca se completar.

  8. Annual Stock Assessment - CWT [Coded Wire Tag program] (USFWS), Annual Report 2007.

    Energy Technology Data Exchange (ETDEWEB)

    Pastor, Stephen M. [U.S. Fish and Wildlife Service, Columbia River Fisheries Program Office

    2009-07-21

    In 1989 the Bonneville Power Administration (BPA) began funding the evaluation of production groups of juvenile anadromous fish not being coded-wire tagged for other programs. These groups were the 'Missing Production Groups'. Production fish released by the U.S. Fish and Wildlife Service (FWS) without representative coded-wire tags during the 1980s are indicated as blank spaces on the survival graphs in this report. This program is now referred to as 'Annual Stock Assessment - CWT'. The objectives of the 'Annual Stock Assessment' program are to: (1) estimate the total survival of each production group, (2) estimate the contribution of each production group to fisheries, and (3) prepare an annual report for USFWS hatcheries in the Columbia River basin. Coded-wire tag recovery information will be used to evaluate the relative success of individual brood stocks. This information can also be used by salmon harvest managers to develop plans to allow the harvest of excess hatchery fish while protecting threatened, endangered, or other stocks of concern. All fish release information, including marked/unmarked ratios, is reported to the Pacific States Marine Fisheries Commission (PSMFC). Fish recovered in the various fisheries or at the hatcheries are sampled to recover coded-wire tags. This recovery information is also reported to PSMFC. This report has been prepared annually starting with the report labeled 'Annual Report 1994'. Although the current report has the title 'Annual Report 2007', it was written in fall of 2008 using data available from RMIS that same year, and submitted as final in January 2009. The main objective of the report is to evaluate survival of groups which have been tagged under this ongoing project.

  9. NERSC Annual Report 2005

    Energy Technology Data Exchange (ETDEWEB)

    Hules (Ed.), John

    2006-07-31

    The National Energy Research Scientific Computing Center (NERSC) is the premier computational resource for scientific research funded by the DOE Office of Science. The Annual Report includes summaries of recent significant and representative computational science projects conducted on NERSC systems as well as information about NERSC's current and planned systems and services.

  10. NERSC Annual Report 2002

    Energy Technology Data Exchange (ETDEWEB)

    Hules, John

    2003-01-31

    The National Energy Research Scientific Computing Center (NERSC) is the primary computational resource for scientific research funded by the DOE Office of Science. The Annual Report for FY2002 includes a summary of recent computational science conducted on NERSC systems (with abstracts of significant and representative projects), and information about NERSC's current and planned systems and service

  11. NRCC annual report, 1979

    Energy Technology Data Exchange (ETDEWEB)

    1980-11-01

    This annual report of the National Research for Computation in Chemistry (NRCC) Division describes the program of research workshops, software development, and scientific research of the Division in 1979. This year marked the first full calendar year of activity of the Division. Initial staffing in the core scientific areas was completed by the addition of a crystallographer.

  12. CSIR Annual report 1966

    CSIR Research Space (South Africa)

    CSIR

    1966-01-01

    Full Text Available This report presents the twenty second annual report of the Council for Scientific and Industrial Research. The report covers the period from the 1st January, 1966, to the 31st December 1966. Balance sheets and statements of income and expenditure...

  13. CSIR Annual report 1970

    CSIR Research Space (South Africa)

    CSIR

    1970-01-01

    Full Text Available This report presents the twenty sixth annual report of the Council for Scientific and Industrial Research. The report covers the period from the 1st January, 1970, to the 31st December 1970. Balance sheets and statements of income and expenditure...

  14. Mail Office annual closure

    CERN Multimedia

    2013-01-01

    On the occasion of the annual closure of CERN, there will be no mail distributed on Friday 20 December 2013 but mail will be collected in the morning. Nevertheless, you will still be able to bring your outgoing mail to Building 555-R-002 until 12 noon.  

  15. Annual Report 2000

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    The Institut Laue-Langevin (ILL) is an international research center using neutrons to probe the microscopic structure and dynamics of a broad range of materials. This annual report presents the ILL activities in 2000: the scientific highlights, the Millennium programme and the new developments, the workshops organized by the ILL, the experimental programme and the publications. (A.L.B.)

  16. 2010 AAUW Annual Report

    Science.gov (United States)

    American Association of University Women, 2010

    2010-01-01

    This report highlights some of the outstanding accomplishments of AAUW (American Association of University Women) for fiscal year 2010. This year's annual report also features stories of remarkable women who are leading the charge to break through barriers and ensure that all women have a fair chance. Sharon is working to reduce the pay gap…

  17. Annual report 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-09-01

    SaskPower`s mission is to meet the electrical needs of Saskatchewan residents in a reliable, safe, efficient and environmentally responsible manner. This annual report of the Corporation discusses new business focus; operations highlights; quality of life; management discussion and analysis; financial ratios; and a report of management. Financial information is also included.

  18. Annual report 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-10-01

    The annual report from IKU (Continental Shelf Institute) in Norway deals with the market adjustment of research activities at the institute as a result of offshore cost-cutting policy in the petroleum industry. The market is about to shift focus from volume to competence. In practice, that means buying competence instead of project ideas or proposals

  19. NERSC Annual Report 2004

    Energy Technology Data Exchange (ETDEWEB)

    Hules, John; Bashor, Jon; Yarris, Lynn; McCullough, Julie; Preuss, Paul; Bethel, Wes

    2005-04-15

    The National Energy Research Scientific Computing Center (NERSC) is the premier computational resource for scientific research funded by the DOE Office of Science. The Annual Report includes summaries of recent significant and representative computational science projects conducted on NERSC systems as well as information about NERSC's current and planned systems and services.

  20. CSIR Annual report 1968

    CSIR Research Space (South Africa)

    CSIR

    1968-01-01

    Full Text Available This report presents the twenty fourth annual report of the Council for Scientific and Industrial Research. The report covers the period from the 1st January, 1968, to the 31st December 1968. Balance sheets and statements of income and expenditure...

  1. CSIR Annual report 1971

    CSIR Research Space (South Africa)

    CSIR

    1971-01-01

    Full Text Available This report presents the twenty seventh annual report of the Council for Scientific and Industrial Research. The report covers the period from the 1st January, 1971, to the 31st December 1971. Balance sheets and statements of income and expenditure...

  2. CSIR Annual report 1969

    CSIR Research Space (South Africa)

    CSIR

    1969-01-01

    Full Text Available This report presents the twenty fifth annual report of the Council for Scientific and Industrial Research. The report covers the period from the 1st January, 1969, to the 31st December 1969. Balance sheets and statements of income and expenditure...

  3. Annual HR Salary Survey.

    Science.gov (United States)

    Schaeffer, Patricia

    2000-01-01

    A trainers' salary survey collected data on 1,091 companies, 31,615 employees, and 97 human resource jobs. Results show pay for human resource professionals is continuing to rise. The survey contains information on base salaries, annual bonuses and incentives, and long-term eligibility incentives. (JOW)

  4. Carolinas Communication Annual, 1998.

    Science.gov (United States)

    McLennan, David B.

    1998-01-01

    This 1998 issue of "Carolinas Communication Annual" contains the following articles: "Give Me That Old Time Religion?: A Study of Religious Themes in the Rhetoric of the Ku Klux Klan" (John S. Seiter); "The Three Stooges versus the Third Reich" (Roy Schwartzman); "Interdisciplinary Team Teaching: Implementing…

  5. UNICEF Annual Report. 1984.

    Science.gov (United States)

    United Nations Children's Fund, New York, NY.

    This annual report reviews the work UNICEF has been doing to help transform the "Child Survival Revolution" from a dream into a reality. Discussion focuses primarily on child health and nutrition and other basic services for children. Throughout, the review is supplemented with profiles of program initiatives made to improve the…

  6. CSIR Annual report 1963

    CSIR Research Space (South Africa)

    CSIR

    1963-01-01

    Full Text Available This report presents the nineteenth annual report of the Council for Scientific and Industrial Research. The report covers the period from the 1st January, 1963, to the 31st December 1963. Balance sheets and statements of income and expenditure...

  7. CSIR Annual report 1982

    CSIR Research Space (South Africa)

    CSIR

    1982-01-01

    Full Text Available This report presents the thirty eighth annual report of the CSIR. The report covers the period 1 January, 1982 to 31 December 1982. A balance sheet and statements of income and expenditure for the financial year ended 31st March 1982, certified...

  8. Uranium industry annual 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-04-22

    The Uranium Industry Annual 1998 (UIA 1998) provides current statistical data on the US uranium industry`s activities relating to uranium raw materials and uranium marketing. It contains data for the period 1989 through 2008 as collected on the Form EIA-858, ``Uranium Industry Annual Survey.`` Data provides a comprehensive statistical characterization of the industry`s activities for the survey year and also include some information about industry`s plans and commitments for the near-term future. Data on uranium raw materials activities for 1989 through 1998, including exploration activities and expenditures, EIA-estimated reserves, mine production of uranium, production of uranium concentrate, and industry employment, are presented in Chapter 1. Data on uranium marketing activities for 1994 through 2008, including purchases of uranium and enrichment services, enrichment feed deliveries, uranium fuel assemblies, filled and unfilled market requirements, and uranium inventories, are shown in Chapter 2. The methodology used in the 1998 survey, including data edit and analysis, is described in Appendix A. The methodologies for estimation of resources and reserves are described in Appendix B. A list of respondents to the ``Uranium Industry Annual Survey`` is provided in Appendix C. The Form EIA-858 ``Uranium Industry Annual Survey`` is shown in Appendix D. For the readers convenience, metric versions of selected tables from Chapters 1 and 2 are presented in Appendix E along with the standard conversion factors used. A glossary of technical terms is at the end of the report. 24 figs., 56 tabs.

  9. CSIR Annual report 1980

    CSIR Research Space (South Africa)

    CSIR

    1980-01-01

    Full Text Available This report presents the thirty-sixth annual report of the CSIR. The report covers the period 1 January, 1980 to 31 December 1980. A balance sheet and statements of income and expenditure for the financial year ended 31st March 1980, certified...

  10. Electric power annual 1996. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-08-01

    The Electric Power Annual presents a summary of electric power industry statistics at national, regional, and State levels. The objective of the publication is to provide industry decisionmakers, government policy-makers, analysts, and the general public with data that may be used in understanding US electricity markets. The Electric Power Annual is prepared by the Coal and Electric Data and Renewables Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); US Department of Energy. Volume 1--with a focus on US electric utilities--contains final 1996 data on net generation and fossil fuel consumption, stocks, receipts, and cost; preliminary 1996 data on generating unit capability, and retail sales of electricity, associated revenue, and the average revenue per kilowatthour of electricity sold. Additionally, information on net generation from renewable energy sources and on the associated generating capability is included in Volume 1 of the EPA. Data published in the Electric Power Annual Volume 1 are compiled from three statistical forms filed monthly and two forms filed annually by electric utilities. These forms are described in detail in the Technical Notes. 5 figs., 30 tabs.

  11. Electric power annual 1995. Volume I

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-07-01

    The Electric Power Annual presents a summary of electric power industry statistics at national, regional, and State levels. The objective of the publication is to provide industry decisionmakers, government policymakers, analysts, and the general public with data that may be used in understanding U.S. electricity markets. The Electric Power Annual is prepared by the Coal and Electric Data and Renewables Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); U.S. Department of Energy. In the private sector, the majority of the users of the Electric Power Annual are researchers and analysts and, ultimately, individuals with policy- and decisionmaking responsibilities in electric utility companies. Financial and investment institutions, economic development organizations interested in new power plant construction, special interest groups, lobbyists, electric power associations, and the news media will find data in the Electric Power Annual useful. In the public sector, users include analysts, researchers, statisticians, and other professionals with regulatory, policy, and program responsibilities for Federal, State, and local governments. The Congress and other legislative bodies may also be interested in general trends related to electricity at State and national levels. Much of the data in these reports can be used in analytic studies to evaluate new legislation. Public service commissions and other special government groups share an interest in State-level statistics. These groups can also compare the statistics for their States with those of other jurisdictions.

  12. LLNL NESHAPs 1998 annual report

    Energy Technology Data Exchange (ETDEWEB)

    Berger, R L; Bertoldo, N A; Biermann, A H; Gallegos, G; Hall, L C; Harrach, R J; Surano, K A

    1999-06-14

    This annual report is prepared pursuant to the National Emissions Standards for Hazardous Air Pollutants (NESHAPs) 40 CFR Part 61, Subpart H; Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an annual effective dose equivalent (EDE) of 10 mrem (100 {micro}Sv) to any member of the public. The EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from 1998 operations are summarized here. (1) Livermore site: 0.055 mrem (0.55 {micro}Sv) (57% from point-source emissions, 43% from diffuse-source emissions). The point-source emissions include gaseous tritium modeled as tritiated water vapor as directed by EPA Region IX and is used for compliance purposes. LLNL believes a more realistic dose for the Livermore site is 0.049 mrem (0.49 {micro}Sv) (52% from point-source emissions, 48% from diffuse-source emissions). This dose is based on an assessment that represents a more realistic behavior of tritium gas in the environment. (2) Site 300: 0.024 mrem (0.24 {micro}Sv) (78% from point-source emissions, 22% from diffuse-source emissions). The EDEs were generally calculated using the EPA-approved CAP88-PC air-dispersion/dose-assessment model. Site-specific meteorological data, stack flow data, and emissions estimates based on radionuclide inventory data or continuous-monitoring systems data were the specific input to CAP88-PC for each modeled source.

  13. LLNL NESHAPs 1998 annual report

    Energy Technology Data Exchange (ETDEWEB)

    Berger, R L; Bertoldo, N A; Biermann, A H; Gallegos, G; Hall, L C; Harrach, R J; Surano, K A

    1999-06-14

    This annual report is prepared pursuant to the National Emissions Standards for Hazardous Air Pollutants (NESHAPs) 40 CFR Part 61, Subpart H; Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an annual effective dose equivalent (EDE) of 10 mrem (100 {micro}Sv) to any member of the public. The EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from 1998 operations are summarized here. (1) Livermore site: 0.055 mrem (0.55 {micro}Sv) (57% from point-source emissions, 43% from diffuse-source emissions). The point-source emissions include gaseous tritium modeled as tritiated water vapor as directed by EPA Region IX and is used for compliance purposes. LLNL believes a more realistic dose for the Livermore site is 0.049 mrem (0.49 {micro}Sv) (52% from point-source emissions, 48% from diffuse-source emissions). This dose is based on an assessment that represents a more realistic behavior of tritium gas in the environment. (2) Site 300: 0.024 mrem (0.24 {micro}Sv) (78% from point-source emissions, 22% from diffuse-source emissions). The EDEs were generally calculated using the EPA-approved CAP88-PC air-dispersion/dose-assessment model. Site-specific meteorological data, stack flow data, and emissions estimates based on radionuclide inventory data or continuous-monitoring systems data were the specific input to CAP88-PC for each modeled source.

  14. Laser nanostructuring of Au/Ag and Au/Ni films for application in SERS

    Science.gov (United States)

    Nikov, Ru. G.; Nedyalkov, N. N.; Atanasov, P. A.; Grochowska, K.; Iwulska, A.; Sliwinski, G.

    2013-03-01

    In this paper results on laser nanostructuring of Au/Ag and Au/Ni thin films are presented. The nanostructuring leads to formation of arrays of bimetallic nanoparticles. The fabrication of the these structures is made using a two step procedure. Initially, thin films are deposited on quartz substrates by classical pulsed laser deposition method. In order to produce Au/Ag or Au/Ni thin films, targets with two sections consist the different metals are used. Thin films with different concentrations of the two metals are obtained by changing the area of the different sections in the target. The as prepared films are then annealed by nanosecond laser pulses delivered by Nd:YAG laser system operated at λ = 355 nm. It is found that the laser annealing may lead to nanostructuring of the deposited films as at certain conditions decomposition into monolayers of nanoparticles with narrow size distribution is obtained. The performed EDX analyses indicate that the fabricated particles are composed by a bimetallic system of the basic metals used. The transmission spectra of the obtained structures show evidences of plasmon excitations. The bimetal nanostructures are covered with Rhodamine 6G and then tested as active substrates for Surface Enhanced Raman Spectroscopy (SERS).

  15. Properties of ordered titanium templates covered with Au thin films for SERS applications

    Science.gov (United States)

    Grochowska, Katarzyna; Siuzdak, Katarzyna; Sokołowski, Michał; Karczewski, Jakub; Szkoda, Mariusz; Śliwiński, Gerard

    2016-12-01

    Currently, roughened metal nanostructures are widely studied as highly sensitive Raman scattering substrates that show application potential in biochemistry, food safety or medical diagnostic. In this work the structural properties and the enhancement effect due to surface enhanced Raman scattering (SERS) of highly ordered nano-patterned titanium templates covered with thin (5-20 nm) gold films are reported. The templates are formed by preparation of a dense structure of TiO2 nanotubes on a flat Ti surface (2 × 2 cm2) and their subsequent etching down to the substrate. SEM images reveal the formation of honeycomb nanostructures with the cavity diameter of 80 nm. Due to the strongly inhomogeneous distribution of the electromagnetic field in the vicinity of the Au film discontinuities the measured average enhancement factor (107-108) is markedly higher than observed for bare Ti templates. The enhancement factor and Raman signal intensity can be optimized by adjusting the process conditions and thickness of the deposited Au layer. Results confirm that the obtained structures can be used in surface enhanced sensing.

  16. Large-Scale Hot Spot Engineering for Quantitative SERS at the Single-Molecule Scale.

    Science.gov (United States)

    Chen, Hung-Ying; Lin, Meng-Hsien; Wang, Chun-Yuan; Chang, Yu-Ming; Gwo, Shangjr

    2015-10-28

    Quantitative surface enhanced Raman spectroscopy (SERS) requires precise control of Raman enhancement factor and detection uniformity across the SERS substrate. Here, we show that alkanethiolate ligand-regulated silver (Ag) nanoparticle films can be used to achieve quantitative SERS measurements down to the single-molecule level. The two-dimensional hexagonal close-packed superlattices of Ag nanoparticles formed in these films allow for SERS detection over a large area with excellent uniformity and high Raman enhancement factor. In particular, the SERS signal from the thiolate ligands on Ag nanoparticle surfaces can be utilized as a stable internal calibration standard for reproducible quantitative measurements. We demonstrate the capability of quantitative SERS by measuring the areal densities of crystal violet molecules embedded in an ultrathin spin-on-glass detection "hot zone", which is a planar and uniformly enhanced region several nanometers above the Ag nanoparticles. The Raman measurement results exhibit a linear response over a wide dynamic range of analyte concentration.

  17. Microwave assisted facile synthesis of reduced graphene oxide-silver (RGO-Ag) nanocomposite and their application as active SERS substrate

    Energy Technology Data Exchange (ETDEWEB)

    Wadhwa, Heena, E-mail: heenawadhwa1988@gmail.com; Kumar, Devender, E-mail: devkumsaroha@kuk.ac.in; Mahendia, Suman, E-mail: mahendia@gmail.com; Kumar, Shyam, E-mail: profshyam@gmail.com

    2017-06-15

    The present paper represents the facile and rapid synthesis of reduced graphene oxide-silver (RGO-Ag) nanocomposite with the help of microwave irradiation. The graphene oxide (GO) solution has been prepared in bulk using Hummer's method followed by microwave assisted in-situ reduction of GO and silver nitrate (AgNO{sub 3}) by hydrazine hydrate in a short spam of 5 min. The prepared nanocomposite has been characterized using Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD) Scanning Electron Microscopy (SEM) and UV–Visible spectroscopy. TEM analysis shows that Ag nanoparticles with average size 32 nm are uniformly entangled with in RGO layers. The UV–Visible absorption spectrum of nanocomposite depicts the reduction of GO to RGO along with the formation of Ag nanoparticles with the presence of characteristic surface Plasmon resonance (SPR) peak of Ag nanoparticles at 422 nm. The performance of prepared nanocomposite has been tested as the active Surface Enhanced Raman Scattering (SERS) substrate for Rhodamine 6G with detection limit 0.1 μM. - Highlights: • The RGO and RGO-Ag nanocomposite were synthesized with microwave irradiation. • Ag nanoparticles of average size 32 nm are uniformly entangled within RGO layers. • RGO itself is a florescence quencher with SERS detection limit 1 μM for R6G. • RGO-Ag nanocomposite show good SERS activity for R6G with detection limit 0.1 μM.

  18. Novel bottom-up SERS substrates for quantitative and parallelized analytics.

    Science.gov (United States)

    Strelau, Katharina K; Schüler, Thomas; Möller, Robert; Fritzsche, Wolfgang; Popp, Jürgen

    2010-02-01

    Surface-enhanced Raman spectroscopy (SERS) is an emerging technology in the field of analytics. Due to the high sensitivity in connection with specific Raman molecular fingerprint information SERS can be used in a variety of analytical, bioanalytical, and biosensing applications. However, for the SERS effect substrates with metal nanostructures are needed. The broad application of this technology is greatly hampered by the lack of reliable and reproducible substrates. Usually the activity of a given substrate has to be determined by time-consuming experiments such as calibration or ultramicroscopic studies. To use SERS as a standard analytical tool, cheap and reproducible substrates are required, preferably with a characterization technique that does not interfere with the subsequent measurements. Herein we introduce an innovative approach to produce low-cost and large-scale reproducible substrates for SERS applications, which allows easy and economical production of micropatterned SERS active surfaces on a large scale. This approach is based on an enzyme-induced growth of silver nanostructures. The special structural feature of the enzymatically deposited silver nanoparticles prevents the breakdown of SERS activity even at high particle densities (particle density >60%) that lead to a conductive layer. In contrast to other approaches, this substrate exhibits a relationship between electrical conductivity and the resulting SERS activity of a given spot. This enables the prediction of the SERS activity of the nanostructure ensemble and therewith the controllable and reproducible production of SERS substrates of enzymatic silver nanoparticles on a large scale, utilizing a simple measurement of the electrical conductivity. Furthermore, through a correlation between the conductivity and the SERS activity of the substrates it is possible to quantify SERS measurements with these substrates.

  19. El ser humano y sus posibilidades de construcción desde el cuidado

    Directory of Open Access Journals (Sweden)

    Alacoque Lorenzini Erdmann

    2003-01-01

    Full Text Available El artículo presenta algunas concepciones del ser humano en relación con el cuidado de enfermería. A partir de la organización de la vida se establecen relaciones, interacciones del ser humano, sus límites y posibilidades para el cuidado con la vida, con lo cotidiano y con la naturaleza. Resalta la importancia y el compromiso en la construcción colectiva del ser ciudadano.

  20. Surface Enhanced Raman Scattering (SERS) Detection of Ammonium Nitrate (AN) Samples Fabricated Using Drop-on-Demand Inkjet Technology on Commercial and Fabricated SERS Substrates

    Science.gov (United States)

    2013-04-01

    Holthoff, E. L.; Stratis-Cullum, D. N.; Hankus, M. E. A Nanosensor for TNT Detection Based on Molecularly Imprinted Polymers and Surface Enhanced Raman...excitation employed, and produces a narrowband spectral signature unique to the 2 molecular vibrations of the analyte. Since the discovery of SERS in...using a simple one-step direct imprinting process, thus, creating a porous gold grating film that has previously been shown to contribute to a SERS

  1. Mesoporous titania based yolk-shell nanoparticles as multifunctional theranostic platforms for SERS imaging and chemo-photothermal treatment

    Science.gov (United States)

    Zhang, Weiwei; Wang, Yunqing; Sun, Xiuyan; Wang, Wenhai; Chen, Lingxin

    2014-11-01

    labeling strategy for the drug delivery system, in which ``empty carriers'' were pre-synthesized, followed by co-loading of Raman reporters on AuNR and anti-cancer drug doxorubicin (DOX) in mTiO2 in sequence. The acquired SERS signal was strong enough for tracking NPs at both living cells and mice levels. Second, we selected mTiO2 as a novel drug loading material instead of the widely used mesoporous silica (mSiO2). The mTiO2 shared satisfactory drug loading and release behavior as mSiO2 but it was chemically inert. This property not only provided a facile way to form a yolk-shell structure but also rendered it with superior structural stability in a biological system. Third, the near infrared (NIR) light absorbing property of the AuNR SERS substrate was also explored for drug release regulation and photothermal treatment. Significantly greater MCF-7 cell killing was observed when treated together with DOX-loaded NPs and NIR laser irradiation, attributable to the synergistic chemo-thermal therapeutic effect. Our results indicated the established SERS labeled yolk-shell NP as a promising theranostic platform and suggested its potential in vivo applications. Electronic supplementary information (ESI) available: The hydrodynamic diameter of the sphere yolk-shell nanoparticles. TEM images of worm-like AuNR@mSiO2@mTiO2 and AuNR@void@mTiO2 NPs. Digital pictures and the Raman spectrum of the DTDC-labeled NP solution prepared under neutral conditions. Raman spectra of SERS labeling NPs by using crystal violet and DTTC as Raman reporters. Zeta potential of AuNR@void@mTiO2 NPs, DTDC labeled NPs and DTDC-DOX co-loaded NPs. See DOI: 10.1039/c4nr04864d

  2. Assignment of vibrational spectral bands of kidney tissue by means of low temperature SERS spectroscopy

    Science.gov (United States)

    Velicka, M.; Radzvilaite, M.; Ceponkus, J.; Urboniene, V.; Pucetaite, M.; Jankevicius, F.; Steiner, G.; Sablinskas, V.

    2017-02-01

    Surface enhanced Raman scattering (SERS) spectroscopy is a useful method for detection of trace amounts of molecules. It has already been successfully implemented for detection of explosives, food additives, biomarkers in blood or urine, etc. In the last decade, SERS spectroscopy was introduced into the field of health sciences and has been especially focused on early disease detection. In the recent years, application of SERS spectroscopy for detection of various types of human cancerous tissues emerged. Furthermore, SERS spectroscopy of extracellular fluid shows great potential for the differentiation of normal and cancerous tissues; however, due to high variety of molecules present in such biological samples, the experimental spectrum is a combination of many different overlapping vibrational spectral bands. Thus, precise assignment of these bands to the corresponding molecular vibrations is a difficult task. In most cases, researchers try to avoid this task satisfying just with tentative assignment. In this study, low temperature SERS measurements of extracellular fluid of cancerous and healthy kidney tissue samples were carried out in order to get a deeper understanding of the nature of vibrational spectral bands present in the experimental spectrum. The SERS spectra were measured in temperature range from 300 K down to 100 K. SERS method was implemented using silver nanoparticle colloidal solution. The results of the low temperature SERS experiment were analysed and compared with the results of theoretical calculations. The analysis showed that the SERS spectrum of extracellular fluid of kidney tissue is highly influenced by the vibrational bands of adenine and Lcystine molecules.

  3. Vibrational fingerprinting of bacterial pathogens by surface enhanced Raman scattering (SERS)

    Science.gov (United States)

    Premasiri, W. Ranjith; Moir, D. T.; Ziegler, Lawrence D.

    2005-05-01

    The surface enhanced Raman scattering (SERS) spectra of vegetative whole-cell bacteria were obtained using in-situ grown gold nanoparticle cluster-covered silicon dioxide substrates excited at 785 nm. SERS spectra of Gram-negative bacteria; E. coli and S. typhimurium, and Gram-positive bacteria; B. subtilis, B. cereus, B. thuringeinsis and B. anthracis Sterne, have been observed. Raman enhancement factors of ~104-105 per cell are found for both Gram positive and Gram negative bacteria on this novel SERS substrate. The bacterial SERS spectra are species specific and exhibit greater species differentiation and reduced spectral congestion than their corresponding non-SERS (bulk) Raman spectra. Fluorescence observed in the 785 nm excited bulk Raman emission of Bacillus species is not apparent in the corresponding SERS spectra. The surface enhancement effect allows the observation of Raman spectra at the single cell level excited by low incident laser powers (blood serum, has an observable effect on the bacterial SERS spectra. However, reproducible, species specific SERS vibrational fingerprints are still obtained. The potential of SERS for detection and identification of bacteria with species specificity on these gold nanoparticle coated substrates is demonstrated by these results.

  4. Sensitive SERS nanotags for use with a hand-held 1064 nm Raman spectrometer

    Science.gov (United States)

    Kearns, Hayleigh; Ali, Fatima; Bedics, Matthew A.; Shand, Neil C.; Faulds, Karen; Detty, Michael R.; Graham, Duncan

    2017-07-01

    This is the first report of the use of a hand-held 1064 nm Raman spectrometer combined with red-shifted surface-enhanced Raman scattering (SERS) nanotags to provide an unprecedented performance in the short-wave infrared (SWIR) region. A library consisting of 17 chalcogenopyrylium nanotags produce extraordinary SERS responses with femtomolar detection limits being obtained using the portable instrument. This is well beyond previous SERS detection limits at this far red-shifted wavelength and opens up new options for SERS sensors in the SWIR region of the electromagnetic spectrum (between 950 and 1700 nm).

  5. Graphene-Plasmonic Hybrid Platform for Label-Free SERS Biomedical Detection

    Science.gov (United States)

    Wang, Pu

    Surface Enhanced Raman Scattering (SERS) has attracted explosive interest for the wealth of vibrational information it provides with minimal invasive effects to target analyte. Nanotechnology, especially in the form of noble metal nanoparticles exhibit unique electromagnetic and chemical characteristics that are explored to realize ultra-sensitive SERS detection in chemical and biological analysis. Graphene, atom-thick carbon monolayer, exhibits superior chemical stability and bio-compatibility. A combination of SERS-active metal nanostructures and graphene will create various synergies in SERS. The main objective of this research was to exploit the applications of the graphene-Au tip hybrid platform in SERS. The hybrid platform consists of a periodic Au nano-pyramid substrate to provide reproducible plasmonic enhancement, and the superimposed monolayer graphene sheet, serving as "built-in" Raman marker. Extensive theoretical and experimental studies were conducted to determine the potentials of the hybrid platform as SERS substrate. Results from both Finite-Domain Time-Domain (FDTD) numerical simulation and Raman scattering of graphene suggested that the hybrid platform boosted a high density of hotspots yielding 1000 times SERS enhancement of graphene bands. Ultra-high sensitivity of the hybrid platform was demonstrated by bio-molecules including dye, protein and neurotransmitters. Dopamine and serotonin can be detected and distinguished at 10-9 M concentration in the presence of human body fluid. Single molecule detection was obtained using a bi-analyte technique. Graphene supported a vibration mode dependent SERS chemical enhancement of ˜10 to the analyte. Quantitative evaluation of hotspots was presented using spatially resolved Raman mapping of graphene SERS enhancement. Graphene plays a crucial role in quantifying SERS hotspots and paves the path for defining SERS EF that could be universally applied to various SERS systems. A reproducible and statistically

  6. TLC-SERS Plates with a Built-In SERS Layer Consisting of Cap-Shaped Noble Metal Nanoparticles Intended for Environmental Monitoring and Food Safety Assurance

    Directory of Open Access Journals (Sweden)

    H. Takei

    2015-01-01

    Full Text Available We report on a thin layer chromatograph (TLC with a built-in surface enhanced Raman scattering (SERS layer for in-situ identification of chemical species separated by TLC. Our goal is to monitor mixture samples or diluted target molecules suspended in a host material, as happens often in environmental monitoring or detection of food additives. We demonstrate that the TLC-SERS can separate mixture samples and provide in-situ SERS spectra. One sample investigated was a mixture consisting of equal portions of Raman-active chemical species, rhodamine 6 G (R6G, crystal violet (CV, and 1,2-di(4-pyridylethylene (BPE. The three components could be separated and their SERS spectra were obtained from different locations. Another sample was skim milk with a trace amount of melamine. Without development, no characteristic peaks were observed, but after development, a peak was observed at 694 cm−1. Unlike previous TLC-SERS whereby noble metal nanoparticles are added after development of a sample, having a built-in SERS layer greatly facilitates analysis as well as maintaining high uniformity of noble metal nanoparticles.

  7. Electric power annual 1997. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-07-01

    The Electric Power Annual presents a summary of electric power industry statistics at national, regional, and State levels. The objective of the publication is to provide industry decisionmakers, government policy-makers, analysts, and the general public with data that may be used in understanding US electricity markets. The Electric Power Annual is prepared by the Electric Power Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); US Department of Energy. Volume 1 -- with a focus on US electric utilities -- contains final 1997 data on net generation and fossil fuel consumption, stocks, receipts, and cost; preliminary 1997 data on generating unit capability, and retail sales of electricity, associated revenue, and the average revenue per kilowatthour of electricity sold (based on a monthly sample: Form EIA-826, ``Monthly Electric Utility Sales and Revenue Report with State Distributions``). Additionally, information on net generation from renewable energy sources and on the associated generating capability is included in Volume 1 of the EPA.

  8. Annual Hanford Site Environmental Permitting Status Report

    Energy Technology Data Exchange (ETDEWEB)

    HOMAN, N.A.

    2000-10-01

    The information contained in, and/or referenced in, this Annual Hanford Site Environmental Permitting Status Report addresses Permit Condition II.W (Other Permits and/or Approvals) of the Dangerous Waste Portion of the Resource Conservation and Recovery Act Permit for the Treatment, Storage, and Disposal of Dangerous Waste, issued by the Washington State Department of Ecology (WA7890008967). Condition II.W specifies that the Permittees are responsible for obtaining all other applicable federal, state, and local permits authorizing the development and operation of the Hanford Facility. This status report also addresses Permit Condition I.E.22, as interpreted in Section 12.1.25 of the Hanford Facility Dangerous Waste Permit Application, General Information Portion (DOE/RL-91-28, Rev. 4), that states this report will be prepared annually and a copy of this report will be placed in the Facility Operating Record, General Information file by October 1 of each year.

  9. 1988 Annual water management plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Ruby Lake NWR 1987 Annual Water Management Report 1988 Annual Water Management Plan. Includes 1987 weather summary, water availability forecast, summary of 1987...

  10. Bi-functional Au/FeS (Au/Co{sub 3}O{sub 4}) composite for in situ SERS monitoring and degradation of organic pollutants

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Shuzhen; Cai, Qian; Lu, Kailing; Liao, Fan, E-mail: fliao@suda.edu.cn; Shao, Mingwang, E-mail: mwshao@suda.edu.cn [Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University (China)

    2016-01-15

    The bi-functional Au/FeS (Au/Co{sub 3}O{sub 4}) composite was fabricated by in situ reducing Au nanoparticles onto the surface of FeS (Co{sub 3}O{sub 4}). The as-prepared FeS possessed a multi-structure composed of plenty of nanoplates, which were coated by Au nanoparticles with an average size of ∼47.5 nm. While the Co{sub 3}O{sub 4} showed a thin hexagonal sheet containing Au nanoparticles on its surface with an average size of ∼79.0 nm. Both the as-prepared Au/FeS and Au/Co{sub 3}O{sub 4} composites exhibited excellent SERS performance, capable of enhancing the Raman signals of R6G molecules with the enhancement factor up to 1.81 × 10{sup 6} and 7.60 × 10{sup 4}, respectively. Moreover, Au/FeS (Au/Co{sub 3}O{sub 4}) composite also has been verified to have intrinsic peroxidase-like activity, which could decompose H{sub 2}O{sub 2} into hydroxyl radicals and then degrade organic pollutants into small molecules. Therefore, SERS can be used to real-time and in situ monitoring the degradation process of R6G molecules, employing the Au/FeS (Au/Co{sub 3}O{sub 4}) composite both as SERS substrate and catalyst. Graphical abstract: SERS was used to real-time and in situ monitoring the degradation of R6G, employing the Au/FeS and Au/Co{sub 3}O{sub 4} composites both as SERS substrates and catalysts.

  11. Highly active surface-enhanced Raman scattering (SERS) substrates based on gold nanoparticles infiltrated into SiO{sub 2} inverse opals

    Energy Technology Data Exchange (ETDEWEB)

    Ankudze, Bright; Philip, Anish [Department of Chemistry, University of Eastern Finland, P.O. Box 111, F1-80101, Joensuu (Finland); Pakkanen, Tuula T., E-mail: Tuula.Pakkanen@uef.fi [Department of Chemistry, University of Eastern Finland, P.O. Box 111, F1-80101, Joensuu (Finland); Matikainen, Antti; Vahimaa, Pasi [Institute of Photonics, University of Eastern Finland, P.O. Box 111, F1-80101, Joensuu (Finland)

    2016-11-30

    Highlights: • SERS substrates prepared by infiltration of nanoparticles into SiO{sub 2} inverse opal. • The SERS substrate gives an enhancement factor of 10{sup 7} for 4-aminothiophenol. • The sensitivity of the substrate is mainly attributed to gold nanoparticle clusters. - Abstract: SiO{sub 2} inverse opal (IO) films with embedded gold nanoparticles (AuNPs) for surface-enhanced Raman scattering (SERS) application are reported. SiO{sub 2} IO films were loaded with AuNPs by a simple infiltration in a single cycle to form Au-SiO{sub 2} IOs. The optical property and the morphology of the Au-SiO{sub 2} IO substrates were characterized; it was observed that they retained the Bragg diffraction of SiO{sub 2} IO and the localized surface plasmon resonance (LSPR) of AuNPs. The SERS property of the Au-SiO{sub 2} IO substrates were studied with methylene blue (MB) and 4-aminothiophenol (4-ATP). The SERS enhancement factors were 10{sup 7} and 10{sup 6} for 4-ATP and MB, respectively. A low detection limit of 10{sup −10} M for 4-ATP was also obtained with the Au-SiO{sub 2} IO substrate. A relative standard deviation of 18.5% for the Raman signals intensity at 1077 cm{sup −1} for 4-ATP shows that the Au-SiO{sub 2} IO substrates have good signal reproducibility. The results of this study indicate that the Au-SiO{sub 2} IO substrates can be used in sensing and SERS applications.

  12. Solution preparation

    Energy Technology Data Exchange (ETDEWEB)

    Seitz, M.G.

    1982-01-01

    Reviewed in this statement are methods of preparing solutions to be used in laboratory experiments to examine technical issues related to the safe disposal of nuclear waste from power generation. Each approach currently used to prepare solutions has advantages and any one approach may be preferred over the others in particular situations, depending upon the goals of the experimental program. These advantages are highlighted herein for three approaches to solution preparation that are currently used most in studies of nuclear waste disposal. Discussion of the disadvantages of each approach is presented to help a user select a preparation method for his particular studies. Also presented in this statement are general observations regarding solution preparation. These observations are used as examples of the types of concerns that need to be addressed regarding solution preparation. As shown by these examples, prior to experimentation or chemical analyses, laboratory techniques based on scientific knowledge of solutions can be applied to solutions, often resulting in great improvement in the usefulness of results.

  13. Coal industry annual 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-01

    Coal Industry Annual 1997 provides comprehensive information about US coal production, number of mines, prices, productivity, employment, productive capacity, and recoverable reserves. US Coal production for 1997 and previous years is based on the annual survey EIA-7A, Coal Production Report. This report presents data on coal consumption, coal distribution, coal stocks, coal prices, and coal quality for Congress, Federal and State agencies, the coal industry, and the general public. Appendix A contains a compilation of coal statistics for the major coal-producing States. This report includes a national total coal consumption for nonutility power producers that are not in the manufacturing, agriculture, mining, construction, or commercial sectors. 14 figs., 145 tabs.

  14. Renewable energy annual 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-01

    The Renewable Energy Annual 1995 is the first in an expected series of annual reports the Energy Information Administration (EIA) intends to publish to provide a comprehensive assessment of renewable energy. This report presents the following information on the history, status, and prospects of renewable energy data: estimates of renewable resources; characterizations of renewable energy technologies; descriptions of industry infrastructures for individual technologies; evaluations of current market status; and assessments of near-term prospects for market growth. An international section is included, as well as two feature articles that discuss issues of importance for renewable energy as a whole. The report also contains a number of technical appendices and a glossary. The renewable energy sources included are biomass (wood), municipal solid waste, biomass-derived liquid fuels, geothermal, wind, and solar and photovoltaic.

  15. Annual Energy Review 2007

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2008-06-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....”

  16. International energy annual 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-04-01

    The International Energy Annual presents an overview of key international energy trends for production, consumption, imports, and exports of primary energy commodities in over 220 countries, dependencies, and areas of special sovereignty. Also included are population and gross domestic product data, as well as prices for crude oil and petroleum products in selected countries. Renewable energy reported in the International Energy Annual includes hydroelectric power and geothermal, solar, and wind electric power. Also included are biomass electric power for Brazil and the US, and biomass, geothermal, and solar energy produced in the US and not used for electricity generation. This report is published to keep the public and other interested parties fully informed of primary energy supplies on a global basis. The data presented have been largely derived from published sources. The data have been converted to units of measurement and thermal values (Appendices E and F) familiar to the American public. 93 tabs.

  17. Petroleum marketing annual 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-24

    The Petroleum Marketing Annual (PMA) provides information and statistical data on a variety of crude oils and refined petroleum products. The publication presents statistics on crude oil costs and refined petroleum products sales for use by industry, government, private sector analysis, educational institutions, and consumers. Data on crude oil include the domestic first purchase price, the fob and landed cost of imported crude oil, and the refiners` acquisition cost of crude oil. Refined petroleum product sales data include motor gasoline, distillates, residuals, aviation fuels, kerosene, and propane. The Petroleum Marketing Division, Office of Oil and Gas, Energy Information Administration ensures the accuracy, quality, and confidentiality of the published data in the Petroleum Marketing Annual. For this production, all estimates have been recalculated since their earlier publication in the Petroleum Marketing Monthly (PMM). These calculations made use of additional data and corrections that were received after the PMM publication date.

  18. NAGRA Annual report 2010

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-04-15

    This annual report presents the highlights of the activities carried out by the Swiss National Co-operative for the Disposal of Radioactive Wastes NAGRA during the year 2010. These include reviews by various commissions of the NAGRA co-operative's proposals for possible sites for nuclear waste repositories. Also, the enhancements made concerning information facilities for the general public at the co-operative's rock laboratories are mentioned. The operation of initial satellite-based precision measurement systems for movements in the earth's crust is noted. Organisational aspects and international co-operation are discussed. This annual report also looks at NAGRA's organisational structures and its commercial accounts. Appendices provide details on waste inventories and volumes and publications made in 2010. A selection of relevant internet addresses is also provided

  19. Annual General Asssembly

    CERN Multimedia

    Pension Fund

    2005-01-01

    All members and beneficiaries of the Pension Fund are invited to attend the Annual General Asssembly to be held in the CERN Council Chamber on Thursday 13 October 2005 at 14:30 The Agenda comprises: Opening Remarks (J. Bezemer) Results and presentation of the Annual Report 2004 - Role of asset classes in pension funds (C. Cuénoud). Copies of the 2004 Report are available from departmental secretariats. Package of measures aiming at equilibrating the Fund - Proposals by the Governing Board (J.-P. Matheys). Questions from members and beneficiaries. Persons wishing to ask questions are encouraged to submit them, where possible, in writing in advance, addressed to Mr C. Cuénoud, Administrator of the Fund. Conclusions (J. Bezemer). As usual, participants are invited to drinks after the assembly. NB The minutes of the 2004 General Assembly are available from the Administration of the Fund (tel.(+4122)767 27 42; e-mail Sophia.Revol@cern.ch)

  20. Annual General Asssembly

    CERN Multimedia

    2005-01-01

    All members and beneficiaries of the Pension Fund are invited to attend the Annual General Asssembly to be held in the CERN Council Chamber on Thursday 13 October 2005 at 14:30 The Agenda comprises: Opening Remarks (J. Bezemer) Results and presentation of the Annual Report 2004 - Role of asset classes in pension funds (C. Cuénoud) Copies of the 2004 Report are available from departmental secretariats. Package of measures aiming at equilibrating the Fund - Proposals by the Governing Board (J.-P. Matheys) Questions from members and beneficiaries Persons wishing to ask questions are encouraged to submit them, where possible, in writing in advance, addressed to Mr C. Cuénoud, Administrator of the Fund. Conclusions (J. Bezemer) As usual, participants are invited to drinks after the assembly. NB The minutes of the 2004 General Assembly are available from the Administration of the Fund (tel.(+4122)767 27 42; e-mail Sophia.Revol@cern.ch)

  1. Aligned Silver Nanorod Array as SERS Substrates for Viral Sensing

    Science.gov (United States)

    Zhao, Yiping; Shanmukh, Saratchandra; Chaney, Stephen B.; Jones, Les; Dluhy, Richard A.; Tripp, Ralph A.

    2006-03-01

    The aligned silver nanorod array substrates prepared by the oblique angle deposition method are capable of providing extremely high enhancement factors (˜10^9) at near-infrared wavelengths (785 nm) for a standard reporter molecule 1,2 trans-(bis)pyridyl-ethene (BPE). The enhancement factor depends strongly on the length of the Ag nanorods, the substrate coating, as well as the polarization of the excitation laser beam. With the current optimum structure, we demonstrate that the detection limit for BPE can be lower than 0.1 fM. The applicability of this substrate to the detection of bioagents has been investigated by looking several viruses, such as Adenovirus, HIV, Rhinovirus and Respiratory Syncytial Virus (RSV), at low quantities (˜0.5uL). Different viruses have different fingerprint Raman spectrum. The detection of virus presented in infected cells has also been demonstrated.

  2. TIARA annual report 1997

    Energy Technology Data Exchange (ETDEWEB)

    Tanaka, Ryuichi; Saido, Masahiro; Nashiyama, Isamu [eds.] [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment] [and others

    1998-10-01

    This annual report describes research activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1997 to March 31, 1998. Summary reports of 90 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  3. NERSC 1998 annual report

    Energy Technology Data Exchange (ETDEWEB)

    Hules, John (ed.)

    1999-03-01

    This 1998 annual report from the National Scientific Energy Research Computing Center (NERSC) presents the year in review of the following categories: Computational Science; Computer Science and Applied Mathematics; and Systems and Services. Also presented are science highlights in the following categories: Basic Energy Sciences; Biological and Environmental Research; Fusion Energy Sciences; High Energy and Nuclear Physics; and Advanced Scientific Computing Research and Other Projects.

  4. TIARA annual report 2000

    Energy Technology Data Exchange (ETDEWEB)

    Saidoh, Masahiro; Toraishi, Akio; Namba, Hideki (eds.) [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment] [and others

    2001-11-01

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2000 to March 31, 2001. Summary reports of 103 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  5. Annual Pension Fund Update

    CERN Multimedia

    Pension Fund

    2011-01-01

    All members and beneficiaries of the Pension Fund are invited to attend the Annual Pension Fund Update to be held in the CERN Council Chamber on Tuesday 20 September 2011 from 10-00 to 12-00 a.m. Copies of the 2010 Financial Statements are available from departmental secretariats. Coffee and croissants will be served prior to the meeting as of 9-30 a.m.

  6. NSLS annual report 1984

    Energy Technology Data Exchange (ETDEWEB)

    Klaffky, R.; Thomlinson, W. (eds.)

    1984-01-01

    The first comprehensive Annual Report of the National Synchrotron Light Source comes at a time of great activity and forward motion for the facility. In the following pages we outline the management changes that have taken place in the past year, the progress that has been made in the commissioning of the x-ray ring and in the enhanced utilization of the uv ring, together with an extensive discussion of the interesting scientific experiments that have been carried out.

  7. 2008 annual merit review

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2009-01-18

    The 2008 DOE Vehicle Technologies Program Annual Merit Review was held February 25-28, 2008 in Bethesda, Maryland. The review encompassed all of the work done by the Vehicle Technologies Program: a total of 280 individual activities were reviewed, by a total of just over 100 reviewers. A total of 1,908 individual review responses were received for the technical reviews, and an additional 29 individual review responses were received for the plenary session review.

  8. TIARA annual report 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-10-01

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1998 to March 31, 1999. Summary reports of 95 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  9. TIARA annual report 1999

    Energy Technology Data Exchange (ETDEWEB)

    Saidoh, Masahiro; Toraishi, Akio; Itoh, Hisayoshi [eds.] [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment] [and others

    2000-10-01

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1999 to March 31, 2000. Summary reports of 106 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  10. TIARA annual report 2001

    Energy Technology Data Exchange (ETDEWEB)

    Saidoh, Masahiro; Ohara, Yoshihiro; Namba, Hideki (eds.) [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment] [and others

    2002-11-01

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2001 to March 31, 2002. Summary reports of 109 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  11. Annual report 1991

    Energy Technology Data Exchange (ETDEWEB)

    Broda, R.; Lesniak, L.; Malecki, P.; Stachura, Z.; Wojciechowski, H. [eds.

    1992-12-31

    The material presented describing the scientific activities of the Henryk Niewodniczanski Institute of Nuclear Physics in 1991 is the first annual report in several years.The arrangement of the report from various departments reflect the present structure of the Institute and is meant to help in the future task of reconstructing the Institute. Few guidelines proposed by the editorial board gave the individual departments much freedom in selecting the material and choosing the volume and format of presentation. (author).

  12. TIARA annual report 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-11-01

    This annual report describes research activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1996 to March 31, 1997. Summary reports of 88 papers and 4 brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (J.P.N.)

  13. El deber ser de la auditoría

    Directory of Open Access Journals (Sweden)

    Omar de Jesús Montilla Galvis

    2006-01-01

    Full Text Available No falta mucho tiempo para asistir a la integración de las sociedades de la Tierra, a llegar a lo que algunos denominan “aldea global”. No podemos detener los cambios, u oponernos a ellos con la idea de que si les somos indiferentes y no les damos importancia no suceden. Las naciones se vienen transformando multidimensionalmente, no es sólo la tecnología, ni los nichos de mercado, nilas formas de producción, ni la competencia en las economías liberadas, es uncambio en la manera cognoscitiva de pensar y actuar en el mundo de hoy. La auditoría es una práctica de trascendental importancia social y económica, permite entablar relaciones de diversa índole entre los agentes económicos, debido a la confianza que se deposita en el trabajo de los contadores públicos cuando ellos extienden su garantía personal o fe pública, respecto del trabajo de investigación denominado auditoría. Es respecto al conocimiento sobre la auditoría que el presente artículo propone hacerle frente a la necesidad de cambio, las adaptaciones y transformaciones con las que sobreviven las especies, la auditoría se ve en grave riesgo de ser absorbida por otras profesiones, motivo por el cual es necesario comenzar un proceso de adaptación para que sobreviva como una práctica propia de los contadores públicos. La adaptación propuesta tiene como finla evolución de la auditoría, “articulando” toda la información derivada del desarrollo del objeto social de las organizaciones para realizar gestión del conocimiento en las mismas, de este modo convertir dicho conocimiento en la ventaja competitiva más importante de la organización. No es posible esperar que la auditoría continúe con las ideas de su génesis antes de la era cristiana, o con los cambios sufridos a causa de la gran depresión, la responsabilidad de la auditoría ahora tiene que ver con el mejoramiento dela sociedad como efecto del cambio de las organizaciones. Es hora de romper el statu quo

  14. Annual Energy Outlook 2009 with Projections to 2030

    Energy Technology Data Exchange (ETDEWEB)

    None

    2009-03-01

    The Annual Energy Outlook 2009 (AEO2009), prepared by the Energy Information Administration (EIA), presents long-term projections of energy supply, demand, and prices through 2030, based on results from EIA’s National Energy Modeling System (NEMS). EIA published an “early release” version of the AEO2009 reference case in December 2008.

  15. Development of surface enhanced Raman scattering (SERS) spectroscopy monitoring of fuel markers to prevent fraud

    Science.gov (United States)

    Wilkinson, Timothy; Clarkson, John; White, Peter C.; Meakin, Nicholas; McDonald, Ken

    2013-05-01

    Governments often tax fuel products to generate revenues to support and stimulate their economies. They also subsidize the cost of essential fuel products. Fuel taxation and subsidization practices are both subject to fraud. Oil marketing companies also suffer from fuel fraud with loss of legitimate sales and additional quality and liability issues. The use of an advanced marking system to identify and control fraud has been shown to be effective in controlling illegal activity. DeCipher has developed surface enhanced Raman scattering (SERS) spectroscopy as its lead technology for measuring markers in fuel to identify and control malpractice. SERS has many advantages that make it highly suitable for this purpose. The SERS instruments are portable and can be used to monitor fuel at any point in the supply chain. SERS shows high specificity for the marker, with no false positives. Multiple markers can also be detected in a single SERS analysis allowing, for example, specific regional monitoring of fuel. The SERS analysis from fuel is also quick, clear and decisive, with a measurement time of less than 5 minutes. We will present results highlighting our development of the use of a highly stable silver colloid as a SERS substrate to measure the markers at ppb levels. Preliminary results from the use of a solid state SERS substrate to measure fuel markers will also be presented.

  16. Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity

    DEFF Research Database (Denmark)

    Jensen, Annie; Løhr, Mille; Eriksen, Louise;

    2012-01-01

    of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype...

  17. A Study of Relationship Between Universality in Fractal Colloids Aggregation and Time-dependent SERS

    Institute of Scientific and Technical Information of China (English)

    张宏光; 刘凡镇; 何天敬; 辛厚文

    1994-01-01

    The relationship between the rule of time-dependent surface-enhanced Raman scattering (SERS) intensity of adsorbed pyridine in AgBr sol and silver bromide colloidal fractal aggregation is studied in this paper. The experimental results support the view of universality in colloidal aggregation and suggest that SERS may be a useful tool in the study of kinetics of colloidal aggregation.

  18. CHEMICALLY DEPOSITED SILVER FILM USED AS A SERS-ACTIVE OVER COATING LAYER FOR POLYMER FILM

    Institute of Scientific and Technical Information of China (English)

    Xiao-ning Liu; Gi Xue; Yun Lu; Jun Zhang; Fen-ting Li; Chen-chen Xue; Stephen Z.D. Cheng

    2001-01-01

    When colloidal silver particles were chemically deposited onto polymer film as an over-coating layer, surfaceenhanced Raman scattering (SERS) spectra could be collected for the surface analysis. SERS measurements of liquid crystal film were successfully performed without disturbing the surface morphology.

  19. SERS-Activated Platforms for Immunoassay: Probes, Encoding Methods, and Applications.

    Science.gov (United States)

    Wang, Zhuyuan; Zong, Shenfei; Wu, Lei; Zhu, Dan; Cui, Yiping

    2017-06-28

    Owing to their excellent multiplexing ability, high sensitivity, and large dynamic range, immunoassays using surface-enhanced Raman scattering (SERS) as the readout signal have found prosperous applications in fields such as disease diagnosis, environmental surveillance, and food safety supervision. Various ever-increasing demands have promoted SERS-based immunoassays from the classical sandwich-type ones to those integrated with fascinating automatic platforms (e.g., test strips and microfluidic chips). As recent years have witnessed impressive progress in SERS immunoassays, we try to comprehensively cover SERS-based immunoassays from their basic working principles to specific applications. Focusing on several basic elements in SERS immunoassays, typical structures of SERS nanoprobes, productive optical spectral encoding strategies, and popular immunoassay platforms are highlighted, followed by their representative biological applications in the last 5 years. Moreover, despite the vast advances achieved to date, SERS immunoassays still suffer from some annoying shortcomings. Thus, proposals on how to improve the SERS immunoassay performance are also discussed, as well as future challenges and perspectives, aiming to give brief and valid guidelines for choosing suitable platforms according to particular applications.

  20. Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors

    DEFF Research Database (Denmark)

    Kneipp, J.; Kneipp, Harald; Wittig, B.

    2010-01-01

    The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold...

  1. Rocky Mountain Arsenal National Wildlife Refuge : Fiscal Year 1994 Annual Progress Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Annual Progress Report was prepared in partial fulfillment of the Cooperative Agreement for Conservation and Management of Fish and Wildlife Resources at the...

  2. Rocky Mountain Arsenal National Wildlife Refuge : Fiscal Year 1997 Annual Progress Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Annual Progress Report was prepared in partial fulfillment of the Cooperative Agreement for Conservation and Management of Fish and Wildlife Resources at the...

  3. Rocky Mountain Arsenal National Wildlife Refuge : Fiscal Year 1999 Annual Progress Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Annual Progress Report was prepared in partial fulfillment of the Cooperative Agreement for Conservation and Management of Fish and Wildlife Resources at the...

  4. Rocky Mountain Arsenal field office : Fiscal Year 1990 Annual Progress Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Annual Progress Report was prepared in partial fulfillment of the Cooperative Agreement for Conservation and Management of Fish and Wildlife Resources at the...

  5. Inhalation Toxicology Research Institute. Annual report, October 1, 1992--September 30, 1993

    Energy Technology Data Exchange (ETDEWEB)

    Nikula, K.J.; Belinsky, S.A.; Bradley, P.L. [eds.

    1993-11-01

    This annual report for the Inhalation Toxicology Research Institute for 1992-1993 consists of 60 individual reports prepared separately by investigators describing progress in their own projects. Most papers are 2-5 pages long.

  6. Rocky Mountain Arsenal National Wildlife Refuge : Fiscal Year 1996 Annual Progress Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Annual Progress Report was prepared in partial fulfillment of the Cooperative Agreement for Conservation and Management of Fish and Wildlife Resources at the...

  7. A Widely Applicable Silver Sol for TLC Detection with Rich and Stable SERS Features

    Science.gov (United States)

    Zhu, Qingxia; Li, Hao; Lu, Feng; Chai, Yifeng; Yuan, Yongfang

    2016-04-01

    Thin-layer chromatography (TLC) coupled with surface-enhanced Raman spectroscopy (SERS) has gained tremendous popularity in the study of various complex systems. However, the detection of hydrophobic analytes is difficult, and the specificity still needs to be improved. In this study, a SERS-active non-aqueous silver sol which could activate the analytes to produce rich and stable spectral features was rapidly synthesized. Then, the optimized silver nanoparticles (AgNPs)-DMF sol was employed for TLC-SERS detection of hydrophobic (and also hydrophilic) analytes. SERS performance of this sol was superior to that of traditional Lee-Meisel AgNPs due to its high specificity, acceptable stability, and wide applicability. The non-aqueous AgNPs would be suitable for the TLC-SERS method, which shows great promise for applications in food safety assurance, environmental monitoring, medical diagnoses, and many other fields.

  8. Gold dimer nanoantenna with slanted gap for tunable LSPR and improved SERS

    KAUST Repository

    Kessentini, Sameh

    2014-02-13

    We focus on improving the surface-enhanced Raman scattering (SERS) of dimer nanoantenna by tailoring the shape of the coupled nanoantennas extremities from rounded to straight or slanted ones. A numerical model based on the discrete dipole approximation method-taking into account periodicity, adhesion layer, and roughness-is first validated by comparison with localized surface plasmon resonance (LSPR) and SERS experiments on round-edged dimer nanoantennas and then used to investigate the effect of the straight or slanted gap in the dimer antenna. Simulations show that both LSPR and SERS can be tuned by changing the gap slanting angle. The SERS enhancement factor can also be improved by 2 orders of magnitude compared to the one reached using a rounded gap. Therefore, the slanting angle can be used as a new control parameter in the design of SERS substrates to guarantee stronger field confinement and higher sensitivity, especially as its feasibility is demonstrated. © 2014 American Chemical Society.

  9. Aromatic amino acids providing characteristic motifs in the Raman and SERS spectroscopy of peptides.

    Science.gov (United States)

    Wei, Fang; Zhang, Dongmao; Halas, Naomi J; Hartgerink, Jeffrey D

    2008-07-31

    Raman and surface-enhanced Raman spectroscopies (SERS) are potentially important tools in the characterization of biomolecules such as proteins and DNA. In this work, SERS spectra of three cysteine-containing aromatic peptides: tryptophan-cysteine, tyrosine-cysteine, and phenylalanine-cysteine, bound to Au nanoshell substrates, were obtained, and compared to their respective normal Raman spectra. While the linewidths of the SERS peaks are significantly broadened (up to 70%), no significant spectral shifts (features in the Raman and SERS spectra of peptides and proteins when present. It follows that the Raman modes of these three small constructed peptides may likely apply to the assignment of Raman and SERS features in the spectra of other peptides and proteins.

  10. Non-labeling multiplex surface enhanced Raman scattering (SERS) detection of volatile organic compounds (VOCs)

    DEFF Research Database (Denmark)

    Wong, Chi Lok; Dinish, U. S.; Schmidt, Michael Stenbæk

    2014-01-01

    In this paper, we report multiplex SERS based VOCs detection with a leaning nano-pillar substrate. The VOCs analyte molecules adsorbed at the tips of the nano-pillars produced SERS signal due to the field enhancement occurring at the localized surface plasmon hot spots between adjacent leaning nano...... chemical sensing layer for the enrichment of gas molecules on sensor surface. The leaning nano-pillar substrate also showed highly reproducible SERS signal in cyclic VOCs detection, which can reduce the detection cost in practical applications. Further, multiplex SERS detection on different combination...... of acetone and ethanol vapor was also successfully demonstrated. The vibrational fingerprints of molecular structures provide specific Raman peaks for different VOCs contents. To the best of our knowledge, this is the first multiplex VOCs detection using SERS. We believe that this work may lead to a portable...

  11. Preparing for the future

    CERN Multimedia

    Panos Charitos

    2016-01-01

    The second annual meeting of the Future Circular Collider (FCC) design study took place from 11 to 15 April in Rome.   The participants in the second annual meeting of the FCC design study. (Photo: Vinicio Tullio/INFN) More than 450 scientists, researchers and leaders of high-tech industry gathered in Rome to review the progress of the Future Circular Collider (FCC) design study. The study was kicked off in 2014 as a response to a statement in the European Strategy for Particle Physics, and today embraces 74 institutes from 26 countries. With the LHC programme well under way, particle physicists are at an exciting juncture. New results from the 13 TeV run could show that we are on the threshold of an eye-opening era that presents new challenges and calls for developments. “To prepare for its future, CERN should continue to develop a vibrant R&D programme that should take advantage of its strengths and uniqueness, pursue design studies for...

  12. One-pot green synthesis of graphene oxide/gold nanocomposites as SERS substrates for malachite green detection.

    Science.gov (United States)

    Fu, Wen Liang; Zhen, Shu Jun; Huang, Cheng Zhi

    2013-05-21

    In this contribution, graphene oxide/gold nanoparticle (GO/AuNPs) hybrids were in situ fabricated through a green one-pot procedure by using tyrosine as an environment friendly and biocompatible reducing agent, which can be used as highly efficient surface enhanced Raman scattering (SERS) substrates with the enhancement factor at 3.8 × 10(3). The as-prepared GO/AuNPs hybrids have good biocompatibility, providing the prospect of applications for biomedicine determinations. In addition, taking the advantages of the electromagnetic and chemical enhancement mechanism and the high affinity of GO and AuNPs towards positive dyes, a sensitive, selective and label-free malachite green (MG) detection method was demonstrated. The SERS measurement showed that the minimum detection concentration of MG in water was as low as 2.5 μmol L(-1) with a linear response range from 2.5 to 100 μmol L(-1) (R(2) = 0.996). Moreover, this method can be applied to detect MG in a fishery water sample with satisfactory results.

  13. The effect of triphenylphosphane on corrosion inhibition of benzotriazole at Ag electrode monitored by SERS in nonaqueous solution

    Science.gov (United States)

    Yuan, Ya-Xian; Yang, Feng-Zhu; Morag, Clark-Heptinstall; Liu, Dong; Zhang, Yong; Gu, Ren-Ao; Yao, Jian-Lin

    2013-03-01

    The corrosion inhibition behavior of benzotriazole (BTAH) on Ag electrodes and the influence of triphenylphosphane (pph3) were investigated by electrochemical method, in situ surface-enhanced Raman spectroscopy (SERS) and direct electrochemical synthesis of surface complexes in nonaqueous solution. The results indicated that the BTA- ion was coordinated to the Ag surface to form a highly cross-linked surface polymer complex of [Ag(BTA)]n, which suppressed the dissolution and oxidation of Ag effectively. The introduction of a neutral ligand of pph3 blocked the surface coordination processes of BTAH with the Ag electrode. It resulted in a decrease of inhibition efficiency to Ag surface. The ligand of pph3 played a negative role on the corrosion inhibition of BTAH to the Ag electrode. The SERS results were well consistent with the cyclic voltammetry and polarization curves measurements. For modeling, two different surface complexes were prepared in acetonitrile with and without pph3 by direct electrochemical synthesis. A polymer-like complex of [Ag(BTA)]n attached to the Ag surface was obtained in the absence of pph3, which suppressed the dissolution and oxidation of Ag effectively. A new binuclear compound, Ag2(BTA)2(pph3)4, was produced in acetonitrile with pph3 and the final coordination process occurred in solution leading to difficulties in forming a compact surface film, thus decreasing the corrosion inhibition efficiency of BTAH. The role of pph3 and the mechanism were proposed.

  14. The effect of triphenylphosphane on corrosion inhibition of benzotriazole at Ag electrode monitored by SERS in nonaqueous solution.

    Science.gov (United States)

    Yuan, Ya-Xian; Yang, Feng-Zhu; Morag, Clark-Heptinstall; Liu, Dong; Zhang, Yong; Gu, Ren-Ao; Yao, Jian-Lin

    2013-03-15

    The corrosion inhibition behavior of benzotriazole (BTAH) on Ag electrodes and the influence of triphenylphosphane (pph(3)) were investigated by electrochemical method, in situ surface-enhanced Raman spectroscopy (SERS) and direct electrochemical synthesis of surface complexes in nonaqueous solution. The results indicated that the BTA(-) ion was coordinated to the Ag surface to form a highly cross-linked surface polymer complex of [Ag(BTA)](n), which suppressed the dissolution and oxidation of Ag effectively. The introduction of a neutral ligand of pph(3) blocked the surface coordination processes of BTAH with the Ag electrode. It resulted in a decrease of inhibition efficiency to Ag surface. The ligand of pph(3) played a negative role on the corrosion inhibition of BTAH to the Ag electrode. The SERS results were well consistent with the cyclic voltammetry and polarization curves measurements. For modeling, two different surface complexes were prepared in acetonitrile with and without pph(3) by direct electrochemical synthesis. A polymer-like complex of [Ag(BTA)](n) attached to the Ag surface was obtained in the absence of pph(3), which suppressed the dissolution and oxidation of Ag effectively. A new binuclear compound, Ag(2)(BTA)(2)(pph(3))(4), was produced in acetonitrile with pph(3) and the final coordination process occurred in solution leading to difficulties in forming a compact surface film, thus decreasing the corrosion inhibition efficiency of BTAH. The role of pph(3) and the mechanism were proposed.

  15. Newly designed silver coated-magnetic, monodisperse polymeric microbeads as SERS substrate for low-level detection of amoxicillin

    Science.gov (United States)

    Kibar, Güneş; Topal, Ahmet Emin; Dana, Aykutlu; Tuncel, Ali

    2016-09-01

    We report the preparation of silver-coated magnetic polymethacrylate core-shell nanoparticles for use in surface-enhanced Raman scattering based drug detection. Monodisperse porous poly (mono-2-(methacryloyloxy)ethyl succinate-co-glycerol dimethacrylate), poly (MMES-co-GDMA) microbeads of ca. 5 μm diameter were first synthesized through a multistage microsuspension polymerization technique to serve as a carboxyl-bearing core region. Microspheres were subsequently magnetized by the co-precipitation of ferric ions, aminated through the surface hydroxyl groups and decorated with Au nanoparticles via electrostatic attraction. An Ag shell was then formed on top of the Au layer through a seed-mediated growth process, resulting in micron-sized monodisperse microbeads that exhibit Raman enhancement effects due to the roughness of the Ag surface layer. The core-shell microspheres were used as a new substrate for the detection of amoxicillin at trace concentrations up to 10-8 M by SERS. The proposed SERS platform can be evaluated as a useful tool for the follow-up amoxicillin pollution and low-level detection of amoxicillin in aqueous media.

  16. DETERMINING THE EDUCATION NEEDS OF TEACHERS IN THE PREPATION OF ANNUAL, UNITE AND DAILY PLANS

    Directory of Open Access Journals (Sweden)

    Gizem BAKAY

    2007-12-01

    Full Text Available This research prepared for establish teachers needs for prepare annual, unite and daily plan. Duringthe research, we use collage teachers and we prepared this survey for collect datas and to determine their needs. We use SPSSprograme with percentage tecnique (% for analyze datas. At the result, in this collage teachers need education for prepareannual and unite plans and they aren’t need any training for prepare daily plan

  17. Facile synthesis of Ag dendrites on Al foil via galvanic replacement reaction with [Ag(NH{sub 3}){sub 2}]Cl for ultrasensitive SERS detecting of biomolecules

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Jiajia; Ye, Weichun [Department of Chemistry, Lanzhou University, Lanzhou 73000 (China); Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 73000 (China); Wang, Chunming, E-mail: wangcm@lzu.edu.cn [Department of Chemistry, Lanzhou University, Lanzhou 73000 (China); Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 73000 (China)

    2013-08-15

    Symmetric silver dendrites have been synthesized on commercial aluminum foil via galvanic replacement reaction with [Ag(NH{sub 3}){sub 2}]Cl. This process is facile and environmentally friendly, without the use of any templates, surfactants or oxidants, and also avoiding the introduction of fluoride anions as a strong toxicity resulting in hypocalcemia. The products were characterized with scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution TEM (HRTEM) and X-ray diffraction (XRD). SEM characterizations and electrochemical measurements including an electrochemical direct current polarization method and OCP-t technique demonstrate that chloride has proven to be the key factor to the formation of well-defined dendritic shape. The as-prepared Ag dendrites are developed as a surface-enhanced Raman scattering (SERS)-active platform for detection of folic acid, DNA and RNA with well resolved bands and high Raman intensities. The detection concentration for the three biomolecules reaches the level of 10{sup −12} M, and thus the symmetric silver dendrites can potentially be employed as effective SERS sensors for label-free and ultrasensitive biomolecule detection. - Highlights: • Simple galvanic replacement is used to synthesize Ag dendrites on commercial Al foils. • This method avoids the introduction of fluoride anions. • The as-prepared dendrites exhibit high SERS activities for biomolecules. • The detection concentration for the biomolecules reaches the level of 10{sup −12} M.

  18. Annual Energy Review 2010

    Energy Technology Data Exchange (ETDEWEB)

    None

    2011-10-01

    This twenty-ninth edition of the Annual Energy Review (AER) presents the U.S. Energy Information Administration’s (EIA) most comprehensive look at integrated energy statistics. The summary statistics on the Nation’s energy production, consumption, trade, stocks, and prices cover all major energy commodities and all energy-consuming sectors of the U.S. economy from 1949 through 2010. The AER is EIA’s historical record of energy statistics and, because the coverage spans six decades, the statistics in this report are well-suited to long-term trend analysis.

  19. Annual Energy Review 2001

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2002-11-01

    The Annual Energy Review (AER) is a statistical history of energy activities in the United States. It documents trends and milestones in U.S. energy production, trade, storage, pricing, and consumption. Each new year of data that is added to the time series—which now reach into 7 decades—extends the story of how Americans have acquired and used energy. It is a story of continual change as the Nation's economy grew, energy requirements expanded, resource availability shifted, and interdependencies developed among nations.

  20. Annual report to Congress

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1992-03-01

    This is the eighth annual report submitted by the Office of Civilian Radioactive Waste Management (OCRWM) to Congress. It covers activities and expenditures during Fiscal Year 1991, which ended September 30, 1991. Chapter 1 of this report describes OCRWM`s mission and objectives. Chapters 2 through 8 cover the following topics: earning public trust and confidence; geological disposal; monitored retrieval storage; transportation; systems integration and regulatory compliance; international programs; and program management. Financial statements for the Nuclear Waste Fund are presented in Chapter 9.

  1. NPL 1999 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    None

    2000-01-01

    OAK-B135 NPL 1999 Annual Report. The Nuclear Physics Laboratory at the University of Washington in Seattle pursues a broad program of nuclear physics research. Research activities are conducted locally and at remote sites. The current program includes ''in-house'' research on nuclear collisions using the local tandem Van de Graaff and superconducting linac accelerators as well as local and remote non-accelerator research on fundamental symmetries and weak interactions and user-mode research on relativistic heavy ions at large accelerator facilities around the world.

  2. Renewable energy annual 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    This report presents summary data on renewable energy consumption, the status of each of the primary renewable technologies, a profile of each of the associated industries, an analysis of topical issues related to renewable energy, and information on renewable energy projects worldwide. It is the second in a series of annual reports on renewable energy. The renewable energy resources included in the report are biomass (wood and ethanol); municipal solid waste, including waste-to-energy and landfill gas; geothermal; wind; and solar energy, including solar thermal and photovoltaic. The report also includes various appendices and a glossary.

  3. Institute annual report 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    The mission of the ITU (Institute for Transuranium Elements) is to protect the European citizen against risk associated with the handling and storage of highly radioactive elements. The JRC (Joint Research Center) provide customer-driven scientific and technical support for the conception, development, implementation and monitoring of EU policies. In this framework this annual report presents the TU actions in: basic actinide research, spent fuel characterization, safety of nuclear fuels, partitioning and transmutation, alpha-immunotherapy/radiobiology, measurement of radioactivity in the environment, safeguards research and development. (A.L.B.)

  4. International energy annual 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    The International Energy Annual presents information and trends on world energy production and consumption for petroleum, natural gas, coal, and electricity. Production and consumption data are reported in standard units as well as British thermal units (Btu). Trade and reserves are shown for petroleum, natural gas, and coal. Data are provided on crude oil refining capacity and electricity installed capacity by type. Prices are included for selected crude oils and for refined petroleum products in selected countries. Population and Gross Domestic Product data are also provided.

  5. Nordel annual report 2003

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-06-01

    Nordel is a body for co-operation between the transmission system operators (TSO's) in Denmark, Finland, Iceland, Norway and Sweden with the primary objective to create conditions for and develop an efficient and harmonised Nordic electricity market, regardless of national borders. Furthermore, Nordel serves as forum for contact between the TSOs and representatives of the market players in the Nordic countries. The statistical part of Nordel's annual report includes data about: Exchange of electricity; Gross consumption; Electricity generation; Installed capacity; Generation of condensing power; Net consumption; Transmission capacity; Pumped storage power; Losses; Occasional power to electric boilers; Total consumption; CHP generation. (BA)

  6. NIRE annual report 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-31

    The National Institute for Resources and Environment (NIRE) has a R & D concept of `ecotechnology` that aims to protect the environment from degradation whilst promoting sustainable development. This annual report presents summaries of 32 recent research efforts on such topics as: emission control of sulfur and nitrogen oxides from advanced coal combustors; catalysts for diesel NO{sub x} removal; measuring dust from stationary sources; software for life cycle assessment; marine disposal of CO{sub 2}; emissions of greenhouse gases from coal mines in Japan; structural changes in coal particles during gasification; solubilization and desulfurization of high sulfur coal with trifluoromethane sulfuroic acid; and oxidation mechanisms of H{sub 2}S.

  7. Nuclear Medicine Annual, 1989

    Energy Technology Data Exchange (ETDEWEB)

    Freeman, L.M.; Weissmann, H.S.

    1989-01-01

    Among the highlights of Nuclear Medicine Annual, 1989 are a status report on the thyroid scan in clinical practice, a review of functional and structural brain imaging in dementia, an update on radionuclide renal imaging in children, and an article outlining a quality assurance program for SPECT instrumentation. Also included are discussions on current concepts in osseous sports and stress injury scintigraphy and on correlative magnetic resonance and radionuclide imaging of bone. Other contributors assess the role of nuclear medicine in clinical decision making and examine medicolegal and regulatory aspects of nuclear medicine.

  8. NERSC 2001 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Hules, John (editor)

    2001-12-12

    The National Energy Research Scientific Computing Center (NERSC) is the primary computational resource for scientific research funded by the DOE Office of Science. The Annual Report for FY2001 includes a summary of recent computational science conducted on NERSC systems (with abstracts of significant and representative projects); information about NERSC's current systems and services; descriptions of Berkeley Lab's current research and development projects in applied mathematics, computer science, and computational science; and a brief summary of NERSC's Strategic Plan for 2002-2005.

  9. Difluoromethane preparation

    NARCIS (Netherlands)

    Wiersma, A.; Sandt, E.J.A.; Van Bekkum, H.; Makkee, M.; Moulijn, J.A.

    1996-01-01

    Abstract of NL 9401574 (A) The invention relates to a method for preparing difluoromethane, wherein dichlorodifluoromethane or monochlorodifluoromethane is brought into contact with hydrogen in the presence of palladium on activated carbon, wherein the loading of the palladium on the activated c

  10. SERS Label Method and its Application in the Biochemical Analysis%SERS 标记方法在生化分析中的应用

    Institute of Scientific and Technical Information of China (English)

    刘建芝; 朱桂池; 胡勇军

    2015-01-01

    Surface-enhanced Raman scattering (SERS)label method is a novel bioassay technology,which combines the modern bio-label technology and SERS spectroscopy.In present method,the signal of the Raman report mole cules, acting as the label tracer signal,is enhanced by adsorbing on the noble metal (i.e.Ag and Au)nanoparticles.With the advantages of high sensitivity,high specificity,fast and easy detection in biochemical analysis,the study on SERS label method has been a hot topic.Here,SERS labeling method has been introduced firstly and its applications recently in the labeled immunoassay,tumor targeting,DNA molecular recognition and microbiological detection have been reviewed.At last,future prospect in this field are indicated.%表面增强拉曼散射(SERS)标记方法结合现代生物标记方法与 SERS 光谱技术,使吸附到金或银等贵金属表面的标记分子的拉曼信号显著增强,并将其作为标记示踪信号,具有生物兼容性好、灵敏度高、分子特征性强和快速简便等优点,已成为新颖的标记示踪技术的研究热点之一。本文综述近年来 SERS 标记技术应用于基因分析、蛋白质检测、微生物检测、肿瘤靶向和小分子物质的最新进展,着重介绍蛋白质和小分子物质的检测,并展望了今后的发展方向。

  11. Cyanuric acid hydrolase from Azorhizobium caulinodans ORS 571: crystal structure and insights into a new class of Ser-Lys dyad proteins.

    Directory of Open Access Journals (Sweden)

    Seunghee Cho

    Full Text Available Cyanuric acid hydrolase (CAH catalyzes the hydrolytic ring-opening of cyanuric acid (2,4,6-trihydroxy-1,3,5-triazine, an intermediate in s-triazine bacterial degradation and a by-product from disinfection with trichloroisocyanuric acid. In the present study, an X-ray crystal structure of the CAH-barbituric acid inhibitor complex from Azorhizobium caulinodans ORS 571 has been determined at 2.7 Å resolution. The CAH protein fold consists of three structurally homologous domains forming a β-barrel-like structure with external α-helices that result in a three-fold symmetry, a dominant feature of the structure and active site that mirrors the three-fold symmetrical shape of the substrate cyanuric acid. The active site structure of CAH is similar to that of the recently determined AtzD with three pairs of active site Ser-Lys dyads. In order to determine the role of each Ser-Lys dyad in catalysis, a mutational study using a highly sensitive, enzyme-coupled assay was conducted. The 10⁹-fold loss of activity by the S226A mutant was at least ten times lower than that of the S79A and S333A mutants. In addition, bioinformatics analysis revealed the Ser226/Lys156 dyad as the only absolutely conserved dyad in the CAH/barbiturase family. These data suggest that Lys156 activates the Ser226 nucleophile which can then attack the substrate carbonyl. Our combination of structural, mutational, and bioinformatics analyses differentiates this study and provides experimental data for mechanistic insights into this unique protein family.

  12. Fabrication of SERS swab for direct detection of trace explosives in fingerprints.

    Science.gov (United States)

    Gong, Zhengjun; Du, Hongjie; Cheng, Fansheng; Wang, Cong; Wang, Canchen; Fan, Meikun

    2014-12-24

    Swab sampling is of great importance in surface contamination analysis. A cotton swab (cotton Q-tip) was successfully transformed into surface-enhanced Raman scattering (SERS) substrate (SERS Q-tip) through a bottom-up strategy, where Ag NPs were first self-assembled onto the Q-tip followed by in situ growing. The capability for direct swab detection of Raman probe Nile Blue A (NBA) and a primary explosive marker 2,4-dinitrotoluene (2,4-DNT) using the SERS Q-tip was explored. It was found that at optimum conditions, a femotogram of NBA on glass surface could be swab-detected. The lowest detectable amount for 2,4-DNT is only ∼1.2 ng/cm(2) (total amount of 5 ng) on glass surface, 2 orders of magnitude more sensitive than similar surface analysis achieved with infrared technique, and comparable even with that obtained by ion mobility spectrometry-mass spectrometry. Finally, 2,4-DNT left on fingerprints was also analyzed. It was found that SERS signal of 2,4-DNT from 27th fingerprint after touching 2,4-DNT powder can still be clearly identified by swabbing with the SERS Q-tip. We believe this is the first direct SERS swabbing test of explosives on fingerprint on glass. Considering its relative long shelf life (>30 d), the SERS Q-tip may find great potential in future homeland security applications when combined with portable Raman spectrometers.

  13. Apoptotic phosphorylation of histone H3 on Ser-10 by protein kinase Cδ.

    Directory of Open Access Journals (Sweden)

    Choon-Ho Park

    Full Text Available Phosphorylation of histone H3 on Ser-10 is regarded as an epigenetic mitotic marker and is tightly correlated with chromosome condensation during both mitosis and meiosis. However, it was also reported that histone H3 Ser-10 phosphorylation occurs when cells are exposed to various death stimuli, suggesting a potential role in the regulation of apoptosis. Here we report that histone H3 Ser-10 phosphorylation is mediated by the pro-apoptotic kinase protein kinase C (PKC δ during apoptosis. We observed that PKCδ robustly phosphorylates histone H3 on Ser-10 both in vitro and in vivo. Ectopic expression of catalytically active PKCδ efficiently induces condensed chromatin structure in the nucleus. We also discovered that activation of PKCδ is required for histone H3 Ser-10 phosphorylation after treatment with DNA damaging agents during apoptosis. Collectively, these findings suggest that PKCδ is the kinase responsible for histone H3 Ser-10 phosphoryation during apoptosis and thus contributes to chromatin condensation together with other apoptosis-related histone modifications. As a result, histone H3 Ser-10 phosphorylation can be designated a new 'apoptotic histone code' mediated by PKCδ.

  14. Tunable coating of gold nanostars: tailoring robust SERS labels for cell imaging

    Science.gov (United States)

    Bassi, B.; Taglietti, A.; Galinetto, P.; Marchesi, N.; Pascale, A.; Cabrini, E.; Pallavicini, P.; Dacarro, G.

    2016-07-01

    Surface modification of noble metal nanoparticles with mixed molecular monolayers is one of the most powerful tools in nanotechnology, and is used to impart and tune new complex surface properties. In imaging techniques based on surface enhanced Raman spectroscopy (SERS), precise and controllable surface modifications are needed to carefully design reproducible, robust and adjustable SERS nanoprobes. We report here the attainment of SERS labels based on gold nanostars (GNSs) coated with a mixed monolayer composed of a poly ethylene glycol (PEG) thiol (neutral or negatively charged) that ensure stability in biological environments, and of a signalling unit 7-Mercapto-4-methylcoumarin as a Raman reporter molecule. The composition of the coating mixture is precisely controlled using an original method, allowing the modulation of the SERS intensity and ensuring overall nanoprobe stability. The further addition of a positively charged layer of poly (allylamine hydrocloride) on the surface of negatively charged SERS labels does not change the SERS response, but it promotes the penetration of GNSs in SH-SY5Y neuroblastoma cells. As an example of an application of such an approach, we demonstrate here the internalization of these new labels by means of visualization of cell morphology obtained with SERS mapping.

  15. Role of Porphyromonas gingivalis SerB in Gingival Epithelial Cell Cytoskeletal Remodeling and Cytokine Production▿

    Science.gov (United States)

    Hasegawa, Yoshiaki; Tribble, Gena D.; Baker, Henry V.; Mans, Jeffrey J.; Handfield, Martin; Lamont, Richard J.

    2008-01-01

    The SerB protein of Porphyromonas gingivalis is a HAD family serine phosphatase that plays a critical role in entry and survival of the organism in gingival epithelial cells. SerB is secreted by P. gingivalis upon contact with epithelial cells. Here it is shown by microarray analysis that SerB impacts the transcriptional profile of gingival epithelial cells, with pathways involving the actin cytoskeleton and cytokine production among those significantly overpopulated with differentially regulated genes. Consistent with the transcriptional profile, a SerB mutant of P. gingivalis exhibited defective remodeling of actin in epithelial cells. Interaction between gingival epithelial cells and isolated SerB protein resulted in actin rearrangement and an increase in the F/G actin ratio. SerB protein was also required for P. gingivalis to antagonize interleukin-8 accumulation following stimulation of epithelial cells with Fusobacterium nucleatum. SerB is thus capable of modulating host cell signal transduction that impacts the actin cytoskeleton and cytokine production. PMID:18391005

  16. Role of Porphyromonas gingivalis SerB in gingival epithelial cell cytoskeletal remodeling and cytokine production.

    Science.gov (United States)

    Hasegawa, Yoshiaki; Tribble, Gena D; Baker, Henry V; Mans, Jeffrey J; Handfield, Martin; Lamont, Richard J

    2008-06-01

    The SerB protein of Porphyromonas gingivalis is a HAD family serine phosphatase that plays a critical role in entry and survival of the organism in gingival epithelial cells. SerB is secreted by P. gingivalis upon contact with epithelial cells. Here it is shown by microarray analysis that SerB impacts the transcriptional profile of gingival epithelial cells, with pathways involving the actin cytoskeleton and cytokine production among those significantly overpopulated with differentially regulated genes. Consistent with the transcriptional profile, a SerB mutant of P. gingivalis exhibited defective remodeling of actin in epithelial cells. Interaction between gingival epithelial cells and isolated SerB protein resulted in actin rearrangement and an increase in the F/G actin ratio. SerB protein was also required for P. gingivalis to antagonize interleukin-8 accumulation following stimulation of epithelial cells with Fusobacterium nucleatum. SerB is thus capable of modulating host cell signal transduction that impacts the actin cytoskeleton and cytokine production.

  17. Annual Energy Review 2004

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2005-08-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  18. Annual Energy Review 2005

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2006-07-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  19. Annual Energy Review 2011

    Energy Technology Data Exchange (ETDEWEB)

    Fichman, Barbara T. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2012-09-01

    The Annual Energy Review (AER) is the U.S. Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are statistics on total energy production, consumption, trade, and energy prices; overviews of petroleum, natural gas, coal, electricity, nuclear energy, and renewable energy; financial and environment indicators; and data unit conversions. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding the content of the AER and other EIA publications.

  20. Annual Energy Review 1993

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-14

    This twelfth edition of the Annual Energy Review (AER) presents the Energy Information Administration`s historical energy statistics. For most series, statistics are given for every year from 1949 through 1993. Because coverage spans four and a half decades, the statistics in this report are well-suited to long-term trend analyses. The AER is comprehensive. It covers all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels and electricity. The AER also presents Energy Information Administration (EIA) statistics on some renewable energy sources. EIA estimates that its consumption series include about half of the renewable energy used in the United States. For a more complete discussion of EIA`s renewables data, see p. xix, ``Introducing Expanded Coverage of Renewable Energy Data Into the Historical Consumption Series.`` Copies of the 1993 edition of the Annual Energy Review may be obtained by using the order form in the back of this publication. Most of the data in the 1993 edition also are available on personal computer diskette. For more information about the diskettes, see the back of this publication. In addition, the data are available as part of the National Economic, Social, and Environmental Data Bank on a CD-ROM. For more information about the data bank, contact the US Department of Commerce Economics and Statistics Administration, on 202-482-1986.

  1. Annual Energy Review 2009

    Energy Technology Data Exchange (ETDEWEB)

    Fichman, Barbara T. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2010-08-01

    The Annual Energy Review (AER) is the U.S. Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are statistics on total energy production, consumption, trade, and energy prices; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, and international energy; financial and environment indicators; and data unit conversions. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding the content of the AER and other EIA publications.

  2. Annual Energy Review 2006

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2007-06-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  3. ANNUAL GENERAL ASSEMBLY

    CERN Multimedia

    2001-01-01

    All members and beneficiaries of the Pension Fund are invited to attend the Annual General Asssembly to be held in the CERN Auditorium on Wednesday 3 October 2001 at 14.30 hrs The Agenda comprises:   Opening Remarks (P. Levaux) Some aspects of risk in a pension fund (C. Cuénoud) Annual Report 2000: Presentation and results (C. Cuénoud) Copies of the Report are available from divisional secretariats. Results of the actuarial reviews (G. Maurin) Questions from members and beneficiaries Persons wishing to ask questions are encouraged to submit them, where possible, in writing in advance, addressed to Mr C. Cuénoud, Administrator of the Fund. Conclusions (P. Levaux) As usual, participants are invited to drinks after the assembly. NB The minutes of the 2000 General Assembly are available from the Administration of the Fund (tel. + 41 22 767 91 94; e-mail Graziella.Praire@cern.ch) The English version will be published next week.

  4. CMS Annual Report 2004

    Energy Technology Data Exchange (ETDEWEB)

    de la Rubia, T D; Shang, S P; Rennie, G; Fluss, M; Westbrook, C

    2005-07-29

    Glance at the articles in this report, and you will sense the transformation that is reshaping the landscape of materials science and chemistry. This transformation is bridging the gaps among chemistry, materials science, and biology--ushering in a wealth of innovative technologies with broad scientific impact. The emergence of this intersection is reinvigorating our strategic investment into areas that build on our strength of interdisciplinary science. It is at the intersection that we position our strategic vision into a future where we will provide radical materials innovations and solutions to our national-security programs and other sponsors. Our 2004 Annual Report describes how our successes and breakthroughs follow a path set forward by our strategic plan and four organizing research themes, each with key scientific accomplishments by our staff and collaborators. We have organized this report into two major sections: research themes and our dynamic teams. The research-theme sections focus on achievements arising from earlier investments while addressing future challenges. The dynamic teams section illustrates the directorate's organizational structure of divisions, centers, and institutes that support a team environment across disciplinary and institutional boundaries. The research presented in this annual report gives substantive examples of how we are proceeding in each of these four theme areas and how they are aligned with our national-security mission. By maintaining an organizational structure that offers an environment of collaborative problem-solving opportunities, we are able to nurture the discoveries and breakthroughs required for future successes.

  5. eenagers Want to be Mothers... But... Las adolescentes quieren ser madres... pero... Adolescentes desejam ser mães… mas…

    Directory of Open Access Journals (Sweden)

    DURÁN DE VILLALOBOS MARÍA MERCEDES

    2010-10-01

    entrevistas a profundidad a 30 adolescentes gestantes, Del análisis emergieron siete categorías: “Embarazo inesperado”, “Asumiendo el embarazo”, “Experimentando formas de cuidarse”, “Padeciendo pérdidas por el embarazo”, “Culpándose por el embarazo”, “Resistiéndose al aborto” y “Reedificando redes de apoyo”. Emerge la categoría central y por ende el planteamiento hipotético afirmando que las adolescentes están “Deseando el embarazo pero no tan pronto”. Se parte del concepto ambivalencia como hilo conductor para mostrar cómo evoluciona esta secuencia de acciones/interacciones en respuesta a la experiencia del embarazo y cómo se alinean según las condiciones y cambios que han experimentado en su contexto también ambivalente.

    Se concluye que las adolescentes embarazadas delegan en el hombre la protección frente a un posible embarazo, sin embargo, cuando se enteran de estar embarazadas los excluyen y excusan de cualquier responsabilidad. Asocian cuidado con cambios en sus comportamientos sociales sin ser significativos los cambios físicos. El 21% de las participantes, están pasando por su segundo embarazo, indicador alarmante para el bienestar de estas jóvenes y sus hijos por nacer. La relación padres-adolescente se afianza durante el embarazo, aunque sus madres cuando tuvieron conocimiento de que sus hijas ya habían iniciado su actividad sexual, lo ignoraron. Se hace un llamado urgente a innovar estrategias de cuidado individualizado y contextualizado.

    Este estudo foi realizado com base teórica e objetivou o seguinte: descrever a experiência de gravidez e gerar uma hipótese sobre esse processo em um grupo de adolescentes; coletar dados através de entrevistas em profundidade realizadas em 30 adolescentes grávidas.

    Sete categorias surgiram da analise: “Gravidez inesperada”; “Assumindo a gravidez”, “Experimentando métodos de prevenção”,

  6. Teenagers Want to be Mothers... But... Las adolescentes quieren ser madres... pero... Adolescentes desejam ser mães… mas…

    Directory of Open Access Journals (Sweden)

    VIRGINIA INÉS SOTO LESMES

    2010-10-01

    álisis emergieron siete categorías: "Embarazo inesperado", "Asumiendo el embarazo", "Experimentando formas de cuidarse", "Padeciendo pérdidas por el embarazo", "Culpándose por el embarazo", "Resistiéndose al aborto" y "Reedificando redes de apoyo". Emerge la categoría central y por ende el planteamiento hipotético afirmando que las adolescentes están "Deseando el embarazo pero no tan pronto". Se parte del concepto ambivalencia como hilo conductor para mostrar cómo evoluciona esta secuencia de acciones/interacciones en respuesta a la experiencia del embarazo y cómo se alinean según las condiciones y cambios que han experimentado en su contexto también ambivalente. Se concluye que las adolescentes embarazadas delegan en el hombre la protección frente a un posible embarazo, sin embargo, cuando se enteran de estar embarazadas los excluyen y excusan de cualquier responsabilidad. Asocian cuidado con cambios en sus comportamientos sociales sin ser significativos los cambios físicos. El 21% de las participantes, están pasando por su segundo embarazo, indicador alarmante para el bienestar de estas jóvenes y sus hijos por nacer. La relación padres-adolescente se afianza durante el embarazo, aunque sus madres cuando tuvieron conocimiento de que sus hijas ya habían iniciado su actividad sexual, lo ignoraron. Se hace un llamado urgente a innovar estrategias de cuidado individualizado y contextualizado.Este estudo foi realizado com base teórica e objetivou o seguinte: descrever a experiência de gravidez e gerar uma hipótese sobre esse processo em um grupo de adolescentes; coletar dados através de entrevistas em profundidade realizadas em 30 adolescentes grávidas. Sete categorias surgiram da analise: "Gravidez inesperada"; "Assumindo a gravidez", "Experimentando métodos de prevenção", "Sofrendo perdas pela gravidez", "Sentindo culpa pela gravidez", "Rejeitando o aborto" e "Reconstruindo redes de apoio". Surge a categoria central e, portanto, a hipótese afirmando que as

  7. Phospho-Bcl-xL(Ser62) influences spindle assembly and chromosome segregation during mitosis.

    Science.gov (United States)

    Wang, Jianfang; Beauchemin, Myriam; Bertrand, Richard

    2014-01-01

    Functional analysis of a series of phosphorylation mutants reveals that Bcl-xL(Ser62Ala) influences cell entry into anaphase and mitotic exit in taxol-exposed cells compared with cells expressing wild-type Bcl-xL or a series of other phosphorylation mutants, an effect that appears to be independent of its anti-apoptotic activity. During normal mitosis progression, Bcl-xL(Ser62) is strongly phosphorylated by PLK1 and MAPK14/SAPKp38α at the prometaphase, metaphase, and the anaphase boundaries, while it is de-phosphorylated at telophase and cytokinesis. Phospho-Bcl-xL(Ser62) localizes in centrosomes with γ-tubulin and in the mitotic cytosol with some spindle-assembly checkpoint signaling components, including PLK1, BubR1, and Mad2. In taxol- and nocodazole-exposed cells, phospho-Bcl-xL(Ser62) also binds to Cdc20- Mad2-, BubR1-, and Bub3-bound complexes, while Bcl-xL(Ser62Ala) does not. Silencing Bcl-xL expression and expressing the phosphorylation mutant Bcl-xL(Ser62Ala) lead to an increased number of cells harboring mitotic spindle defects including multipolar spindle, chromosome lagging and bridging, aneuploidy with micro-, bi-, or multi-nucleated cells, and cells that fail to resolve undergo mitosis within 6 h. Together, the data indicate that during mitosis, Bcl-xL(Ser62) phosphorylation impacts on spindle assembly and chromosome segregation, influencing chromosome stability. Observations of mitotic cells harboring aneuploidy with micro-, bi-, or multi-nucleated cells, and cells that fail to resolve undergo mitosis within 6 h were also made with cells expressing the phosphorylation mutant Bcl-xL(Ser49Ala) and dual mutant Bcl-xL(Ser49/62Ala).

  8. Passive mass transport for direct and quantitative SERS detection using purified silica encapsulated metal nanoparticles

    Science.gov (United States)

    Shrestha, Binaya Kumar

    This thesis focuses on understanding implications of nanomaterial quality control and mass transport through internally etched silica coated nanoparticles for direct and quantitative molecular detection using surface enhanced Raman scattering (SERS). Prior to use, bare nanoparticles (partially or uncoated with silica) are removal using column chromatography to improve the quality of these nanomaterials and their SERS reproducibility. Separation of silica coated nanoparticles with two different diameters is achieved using Surfactant-free size exclusion chromatography with modest fractionation. Next, selective molecular transport is modeled and monitored using SERS and evaluated as a function of solution ionic strength, pH, and polarity. Molecular detection is achieved when the analytes first partition through the silica membrane then interact with the metal surface at short distances (i.e., less than 2 nm). The SERS intensities of unique molecular vibrational modes for a given molecule increases as the number of molecules that bind to the metal surface increases and are enhanced via both chemical and electromagnetic enhancement mechanisms as long as the vibrational mode has a component of polarizability tensor along the surface normal. SERS signals increase linearly with molecular concentration until the three-dimensional SERS-active volume is saturated with molecules. Implications of molecular orientation as well as surface selection rules on SERS intensities of molecular vibrational modes are studied to improve quantitative and reproducible SERS detection using internally etched Ag Au SiO2 nanoparticles. Using the unique vibrational modes, SERS intensities for p-aminothiophenol as a function of metal core compositions and plasmonics are studied. By understanding molecular transport mechanisms through internally etched silica matrices coated on metal nanoparticles, important experimental and materials design parameters are learned, which can be subsequently applied

  9. Federal Power Commission 1977 final annual report

    Energy Technology Data Exchange (ETDEWEB)

    None

    1978-07-01

    This is the 57th and final annual report of the Federal Power Commission. It was prepared so that the Congress and the public will have a full record of the Commission's history. The Commission issued annual reports every year beginning in 1920, except for a five-year period during World War II when abbreviated versions were prepared. The Commission was originally charged with the licensing and regulation of certain uses of the country's water resources, and later was given additional responsibilities, including the regulation of electric rates and services for wholesale transactions, and the regulation of natural gas pipelines and producers, all operating in interstate commerce. The total budget for the Commission in fiscal 1977 was $41,582,000. The Commission's average monthly employment during the year was 1,378. Highlights of 1977 concerning natural gas, electric power, rules and regulations, and legislation are summarized. A history of the FPC is included, followed by in-depth information on natural gas; electric power; legal aspects; and statistics in 4 appendixes. (MCW)

  10. Paper SERS chromatography for detection of trace analytes in complex samples

    Science.gov (United States)

    Yu, Wei W.; White, Ian M.

    2013-05-01

    We report the application of paper SERS substrates for the detection of trace quantities of multiple analytes in a complex sample in the form of paper chromatography. Paper chromatography facilitates the separation of different analytes from a complex sample into distinct sections in the chromatogram, which can then be uniquely identified using SERS. As an example, the separation and quantitative detection of heroin in a highly fluorescent mixture is demonstrated. Paper SERS chromatography has obvious applications, including law enforcement, food safety, and border protection, and facilitates the rapid detection of chemical and biological threats at the point of sample.

  11. Recent progress in the fabrication of SERS substrates based on the arrays of polystyrene nanospheres

    Science.gov (United States)

    Zhang, XiaoLei; Dai, ZhiGao; Zhang, XinGang; Dong, ShiLian; Wu, Wei; Yang, ShiKuan; Xiao, XiangHeng; Jiang, ChangZhong

    2016-12-01

    Micro/nanostructures have broad applications in diverse application fields, such as surface enhanced Raman spectroscopy (SERS), photocatalysis, field emission, photonic crystals, microfluidic devices, electrochemical devices, etc. Using polystyrene (PS) spheres formed monolayer colloidal crystal templates as masks, scaffolds, or molds with different materials growth techniques, many different periodic nanostructured arrays can be obtained with the building units varied from nanoparticles, nanopores, nanorings, nanorods, to nanoshells. Significant progresses have been made on the synthesis of micro/nanostructures with efficient SERS response. In this review, we mainly focus on the various PS template-based fabrication techniques in realizing micro/nanostructured arrays and the SERS applications.

  12. Numerical study on the mechanisms of the SERS of gold-coated pyramidal tip substrates

    Science.gov (United States)

    Li, Rui; Wang, Qiao; Li, Hong; Liu, Kun; Pan, Shi; Zhan, Weishen; Chen, Maodu

    2016-06-01

    In this paper, the physical enhancement mechanisms of the surface-enhanced Raman scattering (SERS) of pyramidal tip substrates are studied theoretically. We structure the periodic square-based arrays of adjacent nanometer pyramidal gold-coated tips on silicon. In order to determine the contribution of plasmonic or diffraction effects on the SERS, three-dimensional (3D) numerical simulations are implemented by taking into account the substrate coated with a gold thin film or a perfect electrical conductor thin film. The tip distance, metal coating thickness and incident light polarization angle are also optimized to investigate whether the further SERS signal can be enhanced.

  13. Aplicaciones de tratamientos de refundido láser en aleaciones metálicas.

    OpenAIRE

    2016-01-01

    Este trabajo de investigación se ha centrado en investigar la tecnología láser como tratamiento de fusión superficial de materiales metálicos, así como analizar las posibles mejoras en sus propiedades. El objetivo concreto del presente trabajo ha sido analizar el empleo de los tratamientos de endurecimiento láser en aleaciones metálicas. Además se ha realizado un procedimiento experimental de a tres muestras con un láser de diodo de alta potencia.

  14. ser y terapia fotodinámica en el tratamiento del acné

    OpenAIRE

    2009-01-01

    De la realización del presente estudio, en le que describimos nuestra experiencia sobre el empleo de láser y terapia fotodinámica en el tratamiento del acné, se extraen las siguientes conclusiones: La terapia fotodinámica, mediante el empleo de mtilaminolevulinato (MAL) y láser de colorante pulsado de pulso largo (LP PDL), es un tratamiento efectivo para el acné de predominio inflamatorio. La terapia fotodinámica en el tratamiento del acné puede ser considerada una técnica inocua dada la prác...

  15. Visiones sobre la alteridad: la lectura sartreana de Ser y tiempo

    OpenAIRE

    Szeftel, Micaela

    2011-01-01

    Al abordar el estudio del Para-otro en El Ser y la Nada, Sartre reconoce la astucia que Heidegger tuvo al haber enunciado la “realidad-humana” como una relación de ser. La oposición entre sujeto y objeto, en la cual las teorías del conocimiento encuentran no sólo el fundamento del existir humano sino también el punto de partida para la pregunta por el ser, es reemplazada por un vínculo fundado en una esfera pre-ontológica. Mientras que Sartre considera que tanto Hegel como Husserl han quedado...

  16. Concepción de ser humano detrás de la Quinta Disciplina

    OpenAIRE

    Roldan Tomasz Suárez Litvin

    2010-01-01

    El presente ensayo expone el modo de ser de un ser humano promovido por la Quinta Disciplina de Peter Senge. Luego de un breve resumen de sus principales postulados, se destaca el modo como los mismos apuntan hacia una forma de humanidad y de relación entre el ámbito individual y el organizacional inconsistente con el esquema de pensamiento social moderno. Seguidamente se muestra que esta concepción no-moderna de ser humano guarda ciertas semejanzas con aquella propia de la cultura griega ant...

  17. Miniaturized monolithic columns for the electrochromatographic separation and SERS detection of molecules of exobiological interest

    Science.gov (United States)

    Carbonnier, Benjamin; Guerrouache, Mohamed

    elegant strategy for preparing multifunctional monoliths through spatially controlled surface functionalization.[9] Photochemical initiation affords spatial control over the reaction site, i.e. site-specific immobilization of ligands on the pore surface of the monolith. Hydrophobic and hydrophilic molecular entities were successfully grafted and the obtained monoliths were applied to electrochromatographic separation application under reversed-phase and hydrophilic interaction electrochromatography modes, respectively. The panel of successfully analyzed analytes ranges from hydrophobic polycyclic aromatic hydrocarbons to life markers such as amino acids and peptides. Our approach was extended to the local design of chelating interface for the site-specific immobilisation of gold nanoparticles. Taking advantage of the unique properties of supported nano-gold, it was possible to perform surface enhanced Raman spectroscopy (SERS) sensing of organic molecules at the sub-nanomolar level. This work is funded by the French Space Agency (CNES) References [1] M.C. Pietrogrande, M.G. Zampolli, F. Dondi, C. Szopa, R. Sternberg, A. Buch, J.F. Raulin, J. Chromatogr. A 1071 (2005) 255. [2] A. M. Stockton, T.M. Chiesl, J.M. Scherer, R. A. Mathies, Anal. Chem. 81 (2009) 790 [3] B. Carbonnier, M. Guerrouache, R. Denoyel, M. C. Millot, J. Sep. Sci. 30 (2007) 3000. [4] J.k. Liu, C.F. Chen, C.W. Chang, D.L. DeVoe, Biosensors Bioelectron. 26 (2010) 182. [5] J. Krenkova, F. Svec, J. Sep. Sci. 32 (2009) 706. [6] F. Svec, J. Chromatogr. B 841 (2004) 52 [7] M. Guerrouache, M. C. Millot, B. Carbonnier, Macromol. Rapid. Commun, 30 (2009) 109. [8] M. Guerrouache, B. Carbonnier, C. Vidal-Madjar, M.C. Millot, J. Chromatogr A, 1149 (2007) 368. [9] M. Guerrouache, S. Mahouche Chergui, M.M. Chehimi, B. Carbonnier. Chem. Commun. 48 (2012) 7486.

  18. Surface-Enhanced Raman Spectroscopy (SERS Tracking of Chelerythrine, a Na+/K+ Pump Inhibitor, into Cytosol and Plasma Membrane Fractions of Human Lens Epithelial Cell Cultures

    Directory of Open Access Journals (Sweden)

    Kevin M. Dorney

    2013-12-01

    Full Text Available Background/Aims: The quaternary benzo-phenanthridine alkaloid (QBA chelerythrine (CET is a pro-apoptotic drug and Na+/K+ pump (NKP inhibitor in human lens epithelial cells (HLECs. In order to obtain further insight into the mechanism of NKP inhibition by CET, its sub-cellular distribution was quantified in cytosolic and membrane fractions of HLEC cultures by surface-enhanced Raman spectroscopy (SERS. Methods: Silver nanoparticles (AgNPs prepared by the Creighton method were concentrated, and size-selected using a one-step tangential flow filtration approach. HLECs cultures were exposed to 50 μM CET in 300 mOsM phosphate-buffered NaCl for 30 min. A variety of cytosolic extracts, crude and purified membranes, prepared in lysing solutions in the presence and absence of a non-ionic detergent, were incubated with AgNPs and subjected to SERS analysis. Determinations of CET were based on a linear calibration plot of the integrated CET SERS intensity at its 659 cm-1 marker band as a function of CET concentration. Results: SERS detected chemically unaltered CET in both cytosol and plasma membrane fractions. Normalized for protein, the CET content was some 100 fold higher in the crude and purified plasma membrane fraction than in the soluble cytosolic extract. The total free CET concentration in the cytosol, free of membranes or containing detergent-solubilized membrane material, approached that of the incubation medium of HLECs. Conclusion: Given a negative membrane potential of HLECs the data suggest, but do not prove, that CET may traverse the plasma membrane as a positively charged monomer (CET+ accumulating near or above passive equilibrium distribution. These findings may contribute to a recently proposed hypothesis that CET binds to and inhibits the NKP through its cytosolic aspect.

  19. Acquisition Workforce Annual Report 2006

    Data.gov (United States)

    General Services Administration — This is the Federal Acquisition Institute's (FAI's) Annual demographic report on the Federal acquisition workforce, showing trends by occupational series, employment...

  20. Acquisition Workforce Annual Report 2008

    Data.gov (United States)

    General Services Administration — This is the Federal Acquisition Institute's (FAI's) Annual demographic report on the Federal acquisition workforce, showing trends by occupational series, employment...

  1. Nuclear Physics Department annual report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    This annual report presents articles and abstracts published in foreign journals, covering the following subjects: nuclear structure, nuclear reactions, applied physics, instrumentation, nonlinear phenomena and high energy physics

  2. Annual Energy Review 1999

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2000-07-01

    A generation ago the Ford Foundation convened a group of experts to explore and assess the Nation’s energy future, and published their conclusions in A Time To Choose: America’s Energy Future (Cambridge, MA: Ballinger, 1974). The Energy Policy Project developed scenarios of U.S. potential energy use in 1985 and 2000. Now, with 1985 well behind us and 2000 nearly on the record books, it may be of interest to take a look back to see what actually happened and consider what it means for our future. The study group sketched three primary scenarios with differing assumptions about the growth of energy use. The Historical Growth scenario assumed that U.S. energy consumption would continue to expand by 3.4 percent per year, the average rate from 1950 to 1970. This scenario assumed no intentional efforts to change the pattern of consumption, only efforts to encourage development of our energy supply. The Technical Fix scenario anticipated a “conscious national effort to use energy more efficiently through engineering know-how." The Zero Energy Growth scenario, while not clamping down on the economy or calling for austerity, incorporated the Technical Fix efficiencies plus additional efficiencies. This third path anticipated that economic growth would depend less on energy-intensive industries and more on those that require less energy, i.e., the service sector. In 2000, total energy consumption was projected to be 187 quadrillion British thermal units (Btu) in the Historical Growth case, 124 quadrillion Btu in the Technical Fix case, and 100 quadrillion Btu in the Zero Energy Growth case. The Annual Energy Review 1999 reports a preliminary total consumption for 1999 of 97 quadrillion Btu (see Table 1.1), and the Energy Information Administration’s Short-Term Energy Outlook (April 2000) forecasts total energy consumption of 98 quadrillion Btu in 2000. What energy consumption path did the United States actually travel to get from 1974, when the scenarios were drawn

  3. Areva, annual report 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    This annual report contains information on AREVA objectives, prospects and strategies, particularly in chapters 4 and 7. This information is a not meant as a presentation of past performance data and should not be interpreted as a guarantee that events or data set forth herein are assured or that objectives will be met. Forward looking statements made in this document also address known and unknown risks, uncertainties and other factors that could, were they to translate into fact, cause AREVA future financial performance, operating performance and production to differ significantly from the objectives presented or suggested herein. Those factors include, in particular, changes in international, economic or market conditions, as well as risk factors presented in Section 4.14.3. Neither AREVA nor the AREVA group is committing to updating forward looking statements or information contained in the annual report. This annual report contains information on the markets, market shares and competitive position of the AREVA group. Unless otherwise indicated, all historical data and forward looking information are based on Group estimates (source: AREVA) and are provided as examples only. To AREVA knowledge, no report is available on the AREVA group markets that is sufficiently complete or objective to serve as a sole reference source. The AREVA group developed estimates based on several sources, including in-house studies and reports, statistics provided by international organizations and professional associations, data published by competitors and information collected by AREVA subsidiaries. The main sources, studies and reports used include (i) the International Atomic Energy Agency (IAEA), the International Energy Agency (IEA), the World Nuclear Association (WNA), the Nuclear Energy Institute (NEA), Nuclear Assurance Corporation (NAC), the European Atomic Energy Community (Euratom) and the Commissariat a l'Energie Atomique (CEA) for the nuclear business; and (ii

  4. La Linguistica Aplicada a la Relacion Paradigmatica entre los Verbos "Ser" y "Estar" (Linguistics Applied to the Paradigmatic Relationship between the Verbs "Ser" and "Estar")

    Science.gov (United States)

    Marchetti, Magda Ruggeri

    1977-01-01

    Speakers of Italian often have problems mastering Spanish because they erroneously believe its great similiarity to Italian makes it easy to learn. One of the fundamental problems is the lack of ability to choose the correct verb, "ser" or "estar," both equivalents of the Italian "essere." (Text is in Spanish.) (CFM)

  5. La Linguistica Aplicada a la Relacion Paradigmatica entre los Verbos "Ser" y "Estar" (Linguistics Applied to the Paradigmatic Relationship between the Verbs "Ser" and "Estar")

    Science.gov (United States)

    Marchetti, Magda Ruggeri

    1977-01-01

    Speakers of Italian often have problems mastering Spanish because they erroneously believe its great similiarity to Italian makes it easy to learn. One of the fundamental problems is the lack of ability to choose the correct verb, "ser" or "estar," both equivalents of the Italian "essere." (Text is in Spanish.) (CFM)

  6. Highly-Sensitive Surface-Enhanced Raman Spectroscopy (SERS)-based Chemical Sensor using 3D Graphene Foam Decorated with Silver Nanoparticles as SERS substrate

    Science.gov (United States)

    Srichan, Chavis; Ekpanyapong, Mongkol; Horprathum, Mati; Eiamchai, Pitak; Nuntawong, Noppadon; Phokharatkul, Ditsayut; Danvirutai, Pobporn; Bohez, Erik; Wisitsoraat, Anurat; Tuantranont, Adisorn

    2016-03-01

    In this work, a novel platform for surface-enhanced Raman spectroscopy (SERS)-based chemical sensors utilizing three-dimensional microporous graphene foam (GF) decorated with silver nanoparticles (AgNPs) is developed and applied for methylene blue (MB) detection. The results demonstrate that silver nanoparticles significantly enhance cascaded amplification of SERS effect on multilayer graphene foam (GF). The enhancement factor of AgNPs/GF sensor is found to be four orders of magnitude larger than that of AgNPs/Si substrate. In addition, the sensitivity of the sensor could be tuned by controlling the size of silver nanoparticles. The highest SERS enhancement factor of ∼5 × 104 is achieved at the optimal nanoparticle size of 50 nm. Moreover, the sensor is capable of detecting MB over broad concentration ranges from 1 nM to 100 μM. Therefore, AgNPs/GF is a highly promising SERS substrate for detection of chemical substances with ultra-low concentrations.

  7. Computation Directorate Annual Report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Crawford, D L; McGraw, J R; Ashby, S F; McCoy, M G; Michels, T C; Eltgroth, P G

    2004-03-12

    Big computers are icons: symbols of the culture, and of the larger computing infrastructure that exists at Lawrence Livermore. Through the collective effort of Laboratory personnel, they enable scientific discovery and engineering development on an unprecedented scale. For more than three decades, the Computation Directorate has supplied the big computers that enable the science necessary for Laboratory missions and programs. Livermore supercomputing is uniquely mission driven. The high-fidelity weapon simulation capabilities essential to the Stockpile Stewardship Program compel major advances in weapons codes and science, compute power, and computational infrastructure. Computation's activities align with this vital mission of the Department of Energy. Increasingly, non-weapons Laboratory programs also rely on computer simulation. World-class achievements have been accomplished by LLNL specialists working in multi-disciplinary research and development teams. In these teams, Computation personnel employ a wide array of skills, from desktop support expertise, to complex applications development, to advanced research. Computation's skilled professionals make the Directorate the success that it has become. These individuals know the importance of the work they do and the many ways it contributes to Laboratory missions. They make appropriate and timely decisions that move the entire organization forward. They make Computation a leader in helping LLNL achieve its programmatic milestones. I dedicate this inaugural Annual Report to the people of Computation in recognition of their continuing contributions. I am proud that we perform our work securely and safely. Despite increased cyber attacks on our computing infrastructure from the Internet, advanced cyber security practices ensure that our computing environment remains secure. Through Integrated Safety Management (ISM) and diligent oversight, we address safety issues promptly and aggressively. The safety of

  8. Annual energy review 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-07-01

    This 13th edition presents the Energy Information Administration`s historical energy statistics. For most series, statistics are given for every year from 1949 through 1994; thus, this report is well-suited to long-term trend analyses. It covers all major energy activities, including consumption, production, trade, stocks, and prices for all major energy commodities, including fossil fuels and electricity. Statistics on renewable energy sources are also included: this year, for the first time, usage of renewables by other consumers as well as by electric utilities is included. Also new is a two-part, comprehensive presentation of data on petroleum products supplied by sector for 1949 through 1994. Data from electric utilities and nonutilities are integrated as ``electric power industry`` data; nonutility power gross generation are presented for the first time. One section presents international statistics (for more detail see EIA`s International Energy Annual).

  9. Coal industry annual 1993

    Energy Technology Data Exchange (ETDEWEB)

    1994-12-06

    Coal Industry Annual 1993 replaces the publication Coal Production (DOE/FIA-0125). This report presents additional tables and expanded versions of tables previously presented in Coal Production, including production, number of mines, Productivity, employment, productive capacity, and recoverable reserves. This report also presents data on coal consumption, coal distribution, coal stocks, coal prices, coal quality, and emissions for a wide audience including the Congress, Federal and State agencies, the coal industry, and the general public. In addition, Appendix A contains a compilation of coal statistics for the major coal-producing States. This report does not include coal consumption data for nonutility Power Producers who are not in the manufacturing, agriculture, mining, construction, or commercial sectors. This consumption is estimated to be 5 million short tons in 1993.

  10. Nagra annual report 2008

    Energy Technology Data Exchange (ETDEWEB)

    Ammann, M. (ed.)

    2009-07-01

    This annual report issued by the Swiss National Co-operative for the Disposal of Radioactive Waste, Nagra, reviews the co-operative's activities in the year 2008 and presents an overview of developments in energy policy, planning procedures and funding plans. Energy policy and the selection of sites for the disposal of radioactive wastes in Switzerland are discussed. Various technical questions are briefly addressed and work being carried out in the rock laboratories in the Swiss Alps and Jura mountains is discussed. International co-operation is reviewed and public relations issues are discussed. Finally, organisational structures are described and Nagra's financial details for the year 2008 are presented and discussed. The report is completed with an appendix containing the co-operative's organigram, waste inventories and listings of publications, addresses and a short glossary.

  11. NIRE annual report 2000

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    The National Institute for Resources and Environment (NIRE) has a R & D concept of 'ecotechnology' that aims to protect the environment from degradation whilst promoting sustainable development. This annual report presents summaries of 38 recent research efforts topics including: life cycle assessment; ocean circulation inverse method for the evaluation of fate of sequestered CO{sub 2} in Western-North Pacific; studies on hydrogasification of coal; computational studies on the reactions of N{sub 2}O with O{sup 3}P and CO; and motion control of bucket of wheel loader based on resistance force at scooping; personal exposure to air pollutants; and development of a quantitative type dust generator. NIRE has coal mine safety research centers at Hokkaido and Kyusyn and an experimental coal mine at Usui. Brief details are given of budget and staff for FY 2000.

  12. Uranium Industry Annual, 1992

    Energy Technology Data Exchange (ETDEWEB)

    1993-10-28

    The Uranium Industry Annual provides current statistical data on the US uranium industry for the Congress, Federal and State agencies, the uranium and electric utility industries, and the public. The feature article, ``Decommissioning of US Conventional Uranium Production Centers,`` is included. Data on uranium raw materials activities including exploration activities and expenditures, resources and reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities including domestic uranium purchases, commitments by utilities, procurement arrangements, uranium imports under purchase contracts and exports, deliveries to enrichment suppliers, inventories, secondary market activities, utility market requirements, and uranium for sale by domestic suppliers are presented in Chapter 2.

  13. Nagra annual report 2007

    Energy Technology Data Exchange (ETDEWEB)

    Ammann, M. (ed.)

    2008-07-01

    This annual report issued by the Swiss National Cooperative for the Disposal of Radioactive Waste reviews the co-operative's activities in the year 2007 and presents an overview of developments in energy policy, planning procedures and funding plans. The selection of sites for the disposal of radioactive wastes in Switzerland is discussed. Various technical questions are briefly addressed and work being carried out in the rock laboratories in the Swiss Alps and Jura mountains is discussed. International co-operation is reviewed and public relations issues are discussed. Finally, organisational structures are described and the financial details for the year 2007 are presented. The report is completed with an appendix containing the co-operative's organigram, waste inventories and listings of publications, addresses and a short glossary.

  14. DANSYNC. Annual report 1996

    Energy Technology Data Exchange (ETDEWEB)

    Als-Nielsen, J.

    1997-02-01

    DANSYNC is an organisation of Danish users of hard X-ray synchrotron facilities, funded by The Danish Natural Science Research Council. It was founded in the beginning of 1996, and this is the first Annual Report from DANSYNC. Users span from basic physics at Risoe National Laboratory, Oersted Laboratory and Denmarks Technical University over materials science from Risoe National Laboratory to chemistry and biology at Aarhus University, Copenhagen University and Denmarks Technical University, as well as industrial research represented by Haldor Topsoee A/S and space research at Danish Space Research Institute. We do not have an X-ray synchrotron facility in Denmark, so all of this work is carried out at facilities abroad. Clearly the facility at DESY in Hamburg (HASYLAB and EMBL) is of the greatest significance for Danish synchrotron research. Home page: http://www.dansync.dk/dansync/. (LN).

  15. 1994 MCAP annual report

    Energy Technology Data Exchange (ETDEWEB)

    Harmony, S.C.; Boyack, B.E.

    1995-04-01

    VELCOR is an integrated, engineering-level computer code that models the progression of severe accidents in light water reactor (LWR) nuclear power plants. The entire spectrum of severe accident phenomena, including reactor coolant system and containment thermal-hydraulic response, core heatup, degradation and relocation, and fission product release and transport is treated in MELCOR in a unified framework for both boiling water reactors (BWRs) and pressurized water reactors (PWRs). Its current uses include the estimation of severe accident source terms and their sensitivities and uncertainties in a variety of applications. Independent assessment efforts have been successfully completed by the US and international MELCOR user communities. Most of these independent assessment efforts have been conducted to support the needs and fulfill the requirements of the individual user organizations. The resources required to perform an extensive set of model and integral code assessments are large. A prudent approach to fostering code development and maturation is to coordinate the individual assessment efforts of the MELCOR user community. While retaining individual control over assessment resources, each organization using the MELCOR code could work with the other users to broaden assessment coverage and minimize duplication. In recognition of these considerations, the US Nuclear Regulatory Commission (US NRC) has initiated the MELCOR Cooperative Assessment Program (MCAP), a vehicle for coordinating and standardizing the assessment practices of the various MELCOR users. In addition, the user community will have a forum to better communicate lessons learned regarding MELCOR applications, capabilities, and user guidelines and limitations and to provide a user community perspective on code development needs and priorities. This second Annual Report builds on the foundation laid with the first Annual Report.

  16. La luminosa sed: Esbozo de un itinerario de la manifestación del ser como deseo de ser al Dios deseante que viene a nuestro encuentro

    Directory of Open Access Journals (Sweden)

    Alberto Toutin

    2009-01-01

    Full Text Available En este artículo se presenta un primer atisbo de un itinerario de pensamiento que busca articular, por un lado, una comprensión de la poesía en Octavio Paz como manifestación del ser humano como apertura al otro, como deseo de ser y, por otro, la comprensión en Hans Urs von Balthasar del acontecimiento de la automanifestación de Dios, como expresión de su deseo de encuentro con el ser humano y que para ello adopta arriesgada y creativamente su lenguaje en la diversidad de sus expresiones. Con ello tan sólo queremos poner algunas bases para una poética teológica, ya iniciada tanto por Dios como por el hablar del ser humano, y que llama de ella misma a ser continuada y profundizada.In this article, the author presents a first glimpse of an itinerary of thought that seeks to articulate, on the one hand, an understanding of poetry by Octavio Paz as a manifestation of the human being as openness to the other and as desire for being and, on the other hand, Hans Urs von Balthasar's understanding of the event of God's self-manifestation as the expression of His desire to meet the human being and, to that end, adopting, with risk and creativity, the human being's language in all the diversity of its expressions. With that, the author only wants to lay some foundations for a theological poetics, already initiated as much by God as by the speech of the human being, and which calls from its own self to be continuous and deepened.

  17. P-Ser-HPr-a link between carbon metabolism and the virulence of some pathogenic bacteria

    DEFF Research Database (Denmark)

    Mijakovic, Ivan

    2005-01-01

    HPr kinase/phosphorylase phosphorylates HPr, a phosphocarrier protein of the phosphoenolpyruvate:carbohydrate phosphotransferase system, at serine-46. P-Ser-HPr is the central regulator of carbon metabolism in Gram-positive bacteria, but also plays a role in virulence development of certain...... pathogens. In Listeria monocytogenes, several virulence genes, which depend on the transcription activator PrfA, are repressed by glucose, fructose, etc., in a catabolite repressor (CcpA)-independent mechanism. However, the catabolite co-repressor P-Ser-HPr was found to inhibit the activity of Prf...... is probably also mediated via P-Ser-HPr, since ccpA disruption leads to elevated amounts of P-Ser-HPr. Indeed, a ccpA ptsH1 double mutant exhibited normal PrfA activity. In S. pyogenes, the expression of several virulence genes depends on the transcription activator Mga. Interestingly, the mga promoter...

  18. Nanoreplicated positive and inverted sub-micron polymer pyramids array for surface enhanced Raman spectroscopy (SERS)

    CERN Document Server

    Xu, Zhida

    2014-01-01

    We demonstrate gold coated polymer surface enhanced Raman scattering (SERS) substrates with a pair of complementary structures--positive and inverted pyramids array structures fabricated by multiple-step molding and replication process. The uniform SERS enhancement factors over the entire device surfaces were measured as 7.2 X 10^4 for positive pyramids substrate while 1.6 X 10^6 for inverted pyramids substrate with Rhodamine 6G as the target analyte. Based on the optical reflection measurement and FDTD simulation result, the enhancement factor difference is attributed to plasmon resonance matching and to SERS "hot spots ". With this simple, fast and versatile complementary molding process, we can produce polymer SERS substrates with extremely low cost, high throughput and high repeatability.

  19. “el ser y el tiempo”, de heidegger continuación (1)

    OpenAIRE

    2011-01-01

    Al hacer la pregunta por el ser, hay que fijar el ente que funciona como primario para formularla; también se requiere establecer la forma recta de acceso a dicho ente.  Entonces, este toma el principal papel en la pregunta.  El" ser ahí" -cada uno de nosotros mismos- es el ente que tiene la preerninenciaóntica y ontológica para hacer la pregunta. Ónticamente es lo más cercano, pero ontológicamente lo más lejano; de la proximidad  proviene la distancia, ya que el "ser ahí", el ser en el mundo...

  20. Reliable Quantitative SERS Analysis Facilitated by Core-Shell Nanoparticles with Embedded Internal Standards.

    Science.gov (United States)

    Shen, Wei; Lin, Xuan; Jiang, Chaoyang; Li, Chaoyu; Lin, Haixin; Huang, Jingtao; Wang, Shuo; Liu, Guokun; Yan, Xiaomei; Zhong, Qiling; Ren, Bin

    2015-06-15

    Quantitative analysis is a great challenge in surface-enhanced Raman scattering (SERS). Core-molecule-shell nanoparticles with two components in the molecular layer, a framework molecule to form the shell, and a probe molecule as a Raman internal standard, were rationally designed for quantitative SERS analysis. The signal of the embedded Raman probe provides effective feedback to correct the fluctuation of samples and measuring conditions. Meanwhile, target molecules with different affinities can be adsorbed onto the shell. The quantitative analysis of target molecules over a large concentration range has been demonstrated with a linear response of the relative SERS intensity versus the surface coverage, which has not been achieved by conventional SERS methods.