WorldWideScience

Sample records for preliminary requirements identification

  1. Preliminary input to the space shuttle reaction control subsystem failure detection and identification software requirements (uncontrolled)

    Science.gov (United States)

    Bergmann, E.

    1976-01-01

    The current baseline method and software implementation of the space shuttle reaction control subsystem failure detection and identification (RCS FDI) system is presented. This algorithm is recommended for conclusion in the redundancy management (RM) module of the space shuttle guidance, navigation, and control system. Supporting software is presented, and recommended for inclusion in the system management (SM) and display and control (D&C) systems. RCS FDI uses data from sensors in the jets, in the manifold isolation valves, and in the RCS fuel and oxidizer storage tanks. A list of jet failures and fuel imbalance warnings is generated for use by the jet selection algorithm of the on-orbit and entry flight control systems, and to inform the crew and ground controllers of RCS failure status. Manifold isolation valve close commands are generated in the event of failed on or leaking jets to prevent loss of large quantities of RCS fuel.

  2. Creativity in Requirement Identification

    DEFF Research Database (Denmark)

    Sørensen, Lene Tolstrup; Olesen, Henning

    Traditional requirements engineering focuses mainly on analysis and elicitation. However, current trends in new system, device and software are towards involving all stakeholders in the early stages of the engineering process to define the user requirements. Creativity is here seen as a major...... keystone in this process in order to open up stakeholder's mind to new technologies, which do not yet exist. This paper dis-cusses the application of creativity in the requirements process and illustrate through cases from the MAGNET and MAGNET Beyond projects....

  3. 31 CFR 103.28 - Identification required.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Identification required. 103.28 Section 103.28 Money and Finance: Treasury Regulations Relating to Money and Finance FINANCIAL RECORDKEEPING AND REPORTING OF CURRENCY AND FOREIGN TRANSACTIONS Reports Required To Be Made §...

  4. On the preliminary psychophysics of fingerprint identification.

    Science.gov (United States)

    Vokey, John R; Tangen, Jason M; Cole, Simon A

    2009-05-01

    For a century, the matching of images of fingerprints has been used for forensic identification. Despite that history, there have been no published, peer-reviewed studies directly examining the extent to which people can correctly match fingerprints to one another. The results of three experiments using naïve undergraduates to match images of fingerprints are reported. The results demonstrate that people can identify fingerprints quite well, and that matching accuracy can vary as a function of both source finger type and image similarity.

  5. Smart gun technology requirements preliminary report

    Energy Technology Data Exchange (ETDEWEB)

    Weiss, D.R.; Brandt, D.J.; Tweet, K.D.

    1995-05-01

    Goal of the Smart Gun Technology project is to eliminate the capability of an unauthorized user from firing a law enforcement officer`s firearm by implementing user-recognizing-and-authorizing surety technologies. This project is funded by the National Institute of Justice. This document reports the projects first objective: to find and document the requirements for a user-recognizing-and-authorizing firearm technology that law enforcement officers will value. This report details the problem of firearm takeaways in law enforcement, the methodology used to develop the law enforcement officers` requirements, and the requirements themselves.

  6. 33 CFR 181.23 - Hull identification numbers required.

    Science.gov (United States)

    2010-07-01

    ... SECURITY (CONTINUED) BOATING SAFETY MANUFACTURER REQUIREMENTS Identification of Boats § 181.23 Hull... identify each boat produced or imported with two hull identification numbers that meet the requirements of... for the purposes of sale, must identify that boat with two hull identification numbers that meet the...

  7. Fluor Daniel Hanford contract standards/requirements identification document

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, G.L.

    1997-04-24

    This document, the Standards/Requirements Identification Document (S/RID) for the Fluor Daniel Hanford Contract, represents the necessary and sufficient requirements to provide an adequate level of protection of the worker, public health and safety, and the environment.

  8. Computer-aided hepatic tumour ablation requirements and preliminary results

    CERN Document Server

    Voirin, D; Amavizca, M; Letoublon, C; Troccaz, J; Voirin, David; Payan, Yohan; Amavizca, Miriam; Letoublon, Christian; Troccaz, Jocelyne

    2002-01-01

    Surgical resection of hepatic tumours is not always possible, since it depends on different factors, among which their location inside the liver functional segments. Alternative techniques consist in local use of chemical or physical agents to destroy the tumour. Radio frequency and cryosurgical ablations are examples of such alternative techniques that may be performed percutaneously. This requires a precise localisation of the tumour placement during ablation. Computer-assisted surgery tools may be used in conjunction with these new ablation techniques to improve the therapeutic efficiency, whilst they benefit from minimal invasiveness. This paper introduces the principles of a system for computer-assisted hepatic tumour ablation and describes preliminary experiments focusing on data registration evaluation. To keep close to conventional protocols, we consider registration of pre-operative CT or MRI data to intra-operative echographic data.

  9. Electronic Warfare M-on-N Digital Simulation Logging Requirements and HDF5: A Preliminary Analysis

    Science.gov (United States)

    2017-04-12

    E. Jarvis Electronic Warfare M-on- N Digital Simulation Logging Requirements and HDF5: A Preliminary Analysis Advanced Techniques Branch Tactical...12-04-2017 NRL Memorandum Report Electronic Warfare M-on- N Digital Simulation Logging Requirements and HDF5: A Preliminary Analysis Donald E...ELECTRONIC WARFARE M-ON- N DIGITAL SIMULATION LOGGING REQUIREMENTS AND HDF5: A PRELIMINARY ANALYSIS 1. INTRODUCTION HDF5 technology [Folk] has been

  10. Identification of Requirements using Goal Oriented Requirements Elicitation Process

    National Research Council Canada - National Science Library

    Mohd Arif; Saoud Sarwar

    2015-01-01

    .... The objective of this paper is twofold; firstly, we classify different requirements elicitation techniques on the basis of different criterion like traditional, cognitive, collaborative, and contextual techniques...

  11. 5 CFR 2504.9 - Identification requirements.

    Science.gov (United States)

    2010-01-01

    ... consulting with the appropriate system manager, may require further reasonable assurances, such as statements... the Privacy Act Officer, after consulting with the appropriate system manager, the granting of...

  12. Preliminary Concept of Operations for a Global Cylinder Identification and Monitoring System

    Energy Technology Data Exchange (ETDEWEB)

    Whitaker, J. M. [ORNL; White-Horton, J. L. [ORNL; Morgan, J. B. [InSolves Associates

    2013-08-01

    This report describes a preliminary concept of operations for a Global Cylinder Identification and Monitoring System that could improve the efficiency of the International Atomic Energy Agency (IAEA) in conducting its current inspection activities and could provide a capability to substantially increase its ability to detect credible diversion scenarios and undeclared production pathways involving UF6 cylinders. There exist concerns that a proliferant State with access to enrichment technology could obtain a cylinder containing natural or low-enriched uranium hexafluoride (UF6) and produce a significant quantity (SQ)1 of highly enriched uranium in as little as 30 days. The National Nuclear Security Administration (NNSA) through the Next Generation Safeguards Initiative sponsored a multi-laboratory team to develop an integrated system that provides for detecting scenarios involving 1) diverting an entire declared cylinder for enrichment at a clandestine facility, 2) misusing a declared cylinder at a safeguarded facility, and 3) using an undeclared cylinder at a safeguarded facility. An important objective in developing this integrated system was to improve the timeliness for detecting the cylinder diversion and undeclared production scenarios. Developing this preliminary concept required in-depth analyses of current operational and safeguards practices at conversion, enrichment, and fuel fabrication facilities. The analyses evaluated the processing, movement, and storage of cylinders at the facilities; the movement of cylinders between facilities (including cylinder fabrication); and the misuse of safeguarded facilities.

  13. 5 CFR 1630.7 - Identification requirements.

    Science.gov (United States)

    2010-01-01

    ... also access the TSP Web site or call the TSP ThriftLine to obtain account information. These systems... or herself by signature, i.e., to note by signature the date of access, Social Security number, and... Act Officer or record keeper designee after consulting with the appropriate system manager may require...

  14. Preliminary identification of problem soils for infrastructure projects

    CSIR Research Space (South Africa)

    Paige-Green, P

    2008-11-01

    Full Text Available soils are those within the top 1.0m or 1.5m of the soil profile, a mechanism for evaluating these materials without preliminary filed work and testing would be invaluable. Since 1971, the Department of Agriculture has systematically mapped the soils...

  15. B plant standards/requirements identification document (S/RID)

    Energy Technology Data Exchange (ETDEWEB)

    Maddox, B.S., Westinghouse Hanford

    1996-07-29

    This Standards/Requirements Identification Document (S/RID) set forth the Environmental Safety and Health (ES{ampersand}H) standards/requirements for the B Plant. This S/RID is applicable to the appropriate life cycle phases of design, construction,operation, and preparation for decommissioning. These standards/requirements are adequate to ensure the protection of the health and safety of workers, the public, and the environment.

  16. MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY

    Directory of Open Access Journals (Sweden)

    A. Petruzzelli

    2011-01-01

    Full Text Available The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and culturebased methods. The estimation of genotypic intra-species variation of the autochthonous bacteria isolated was also evaluated by using randomly amplified polymorphic DNA (RAPD analysis and unweighted pairgroup method with arithmetic averages (UPGMA cluster analysis. Our findings revealed an high diversity of the autochthonous bacterial population investigated, both at species and strain level.

  17. Computational requirements for on-orbit identification of space systems

    Science.gov (United States)

    Hadaegh, Fred Y.

    1988-01-01

    For the future space systems, on-orbit identification (ID) capability will be required to complement on-orbit control, due to the fact that the dynamics of large space structures, spacecrafts, and antennas will not be known sufficiently from ground modeling and testing. The computational requirements for ID of flexible structures such as the space station (SS) or the large deployable reflectors (LDR) are however, extensive due to the large number of modes, sensors, and actuators. For these systems the ID algorithm operations need not be computed in real-time, only in near real-time, or an appropriate mission time. Consequently the space systems will need advanced processors and efficient parallel processing algorithm design and architectures to implement the identification algorithms in near real-time. The MAX computer currently being developed may handle such computational requirements. The purpose is to specify the on-board computational requirements for dynamic and static identification for large space structures. The computational requirements for six ID algorithms are presented in the context of three examples: the JPL/AFAL ground antenna facility, the space station (SS), and the large deployable reflector (LDR).

  18. [Utilization of LIN (lysine-indole-motility) medium for the preliminary identification of enteropathogenic bacteria].

    Science.gov (United States)

    Bockemühl, J; Bednarek, I

    1975-07-01

    A multiple-test medium for the routine laboratory identification of enteropathogenic bacteria is described. The medium has the following formula: Bacto-peptone (Difco) 5 g; yeast extract (Difco) 3 g; casein tryptic digest peptone (Merek) 15 g; glucose 1 g; L-lysine-monohydrochloride (Merck, No. 5700) 5 g; NaCl 5 g; bromcresol purple 0.016 g; agar 2 g; distilled water 1000 ml; final pH 6.6. The medium is dispensed in amounts of 5 ml into tubes of 14 X 85 mm and autoclaved at 120 degrees C for 10 min. The tubes are tightly closed with rubber stoppers. - The medium is inoculated by stabbing to the bottom of the tube. Readings are made after over-night incubation at 37 degrees C. A scheme for the preliminary identification of enteropathogenic bacteria is given, based on LIM medium in conjunction with Kligler's iron agar, and the oxidase reaction.

  19. Preliminary Requirement of Hot Pool Free Surface Level from PGSFR Reactor Head

    Energy Technology Data Exchange (ETDEWEB)

    Koo, Gyeonghoi; Joo, Hyeongkook [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-05-15

    The sensitivity study on structural integrity evaluations are carried out to make a decision of a hot pool free surface location from the reactor head for a preliminary designed reactor enclosure system. To do this, the thermal stress evaluations for a reactor vessel are carried out for a steady state normal operating condition with detailed heat transfer analyses through the reactor enclosure system. From these results, the preliminary design requirement of a hot pool free surface location from the reactor head is established to be 2.0m. From the sensitivity studies on the structural integrity evaluations for a steady state condition, the preliminary distance from the hot pool free surface to the reactor head is determined to be 2.0m same as a conceptual design. More detailed structural analyses for a reactor enclosure system will be carried out as a PGSFR structural design goes forward in detail.

  20. Hippocampal brain volume is associated with faster facial emotion identification in older adults: preliminary results

    Directory of Open Access Journals (Sweden)

    Sarah M Szymkowicz

    2016-08-01

    Full Text Available Fast correct identification of facial emotions is highly relevant for successful social interactions. Research suggests that older, compared to young, adults experience increased difficulty with face and emotion processing skills. While functional neuroimaging studies suggest age differences in neural processing of faces and emotions, evidence about age-associated structural brain changes and their involvement in face and emotion processing is scarce. Using structural magnetic resonance imaging, this study investigated the extent to which volumes of frontal and temporal brain structures were related to reaction time in accurate identification of facial emotions in 30 young and 30 older adults. Volumetric segmentation was performed using FreeSurfer and gray matter volumes from frontal and temporal regions were extracted. Analyses of covariance models with response time as the dependent variable and age group and regional volume, and their interaction, as independent variables were conducted, controlling for total intracranial volume. Results indicated that, in older adults, larger hippocampal volumes were associated with faster correct facial emotion identification. These preliminary observations suggest that greater volume in brain regions associated with face and emotion processing contributes to improved facial emotion identification performance in aging.

  1. Hippocampal Brain Volume Is Associated with Faster Facial Emotion Identification in Older Adults: Preliminary Results.

    Science.gov (United States)

    Szymkowicz, Sarah M; Persson, Jonas; Lin, Tian; Fischer, Håkan; Ebner, Natalie C

    2016-01-01

    Quick correct identification of facial emotions is highly relevant for successful social interactions. Research suggests that older, compared to young, adults experience increased difficulty with face and emotion processing skills. While functional neuroimaging studies suggest age differences in neural processing of faces and emotions, evidence about age-associated structural brain changes and their involvement in face and emotion processing is scarce. Using structural magnetic resonance imaging (MRI), this study investigated the extent to which volumes of frontal and temporal brain structures were related to reaction time in accurate identification of facial emotions in 30 young and 30 older adults. Volumetric segmentation was performed using FreeSurfer and gray matter volumes from frontal and temporal regions were extracted. Analysis of covariances (ANCOVAs) models with response time (RT) as the dependent variable and age group and regional volume, and their interaction, as independent variables were conducted, controlling for total intracranial volume (ICV). Results indicated that, in older adults, larger hippocampal volumes were associated with faster correct facial emotion identification. These preliminary observations suggest that greater volume in brain regions associated with face and emotion processing contributes to improved facial emotion identification performance in aging.

  2. Computer-Aided Identification and Validation of Privacy Requirements

    Directory of Open Access Journals (Sweden)

    Rene Meis

    2016-05-01

    Full Text Available Privacy is a software quality that is closely related to security. The main difference is that security properties aim at the protection of assets that are crucial for the considered system, and privacy aims at the protection of personal data that are processed by the system. The identification of privacy protection needs in complex systems is a hard and error prone task. Stakeholders whose personal data are processed might be overlooked, or the sensitivity and the need of protection of the personal data might be underestimated. The later personal data and the needs to protect them are identified during the development process, the more expensive it is to fix these issues, because the needed changes of the system-to-be often affect many functionalities. In this paper, we present a systematic method to identify the privacy needs of a software system based on a set of functional requirements by extending the problem-based privacy analysis (ProPAn method. Our method is tool-supported and automated where possible to reduce the effort that has to be spent for the privacy analysis, which is especially important when considering complex systems. The contribution of this paper is a semi-automatic method to identify the relevant privacy requirements for a software-to-be based on its functional requirements. The considered privacy requirements address all dimensions of privacy that are relevant for software development. As our method is solely based on the functional requirements of the system to be, we enable users of our method to identify the privacy protection needs that have to be addressed by the software-to-be at an early stage of the development. As initial evaluation of our method, we show its applicability on a small electronic health system scenario.

  3. Identification and preliminary evaluation of polychlorinated naphthalene emissions from hot dip galvanizing plants.

    Science.gov (United States)

    Liu, Guorui; Lv, Pu; Jiang, Xiaoxu; Nie, Zhiqiang; Liu, Wenbin; Zheng, Minghui

    2015-01-01

    Hot dip galvanizing (HDG) processes are sources of polychlorinated-p-dioxins and dibenzofurans (PCDD/Fs). Close correlations have been found between the concentration of PCDD/Fs and polychlorinated naphthalenes (PCNs) that are produced and released during industrial thermal processes. We speculated, therefore, that HDG plants are potential PCN sources. In this preliminary study, PCNs were analyzed in solid residues, ash and precipitate from three HDG plants of different sizes. The total PCN concentrations (∑2-8PCNs) in the residue samples ranged from 60.3 to 226pgg(-1). The PCN emission factors for the combined ash and precipitate residues from the HDG plants ranged from 75 to 178ngt(-1) for the dichlorinated and octachlorinated naphthalenes. The preliminary results suggested that the HDG industry might not currently be a significant source of PCN emissions. The trichloronaphthalenes were the dominant homologs followed by the dichloronaphthalenes and the tetrachloronaphthalenes. The PCN congeners CN37/33/34, CN52/60, CN66/67, and CN73 dominated the tetrachlorinated, pentachlorinated, hexachlorinated, and heptachlorinated naphthalene homologs, respectively. The PCNs emitted from the HDG plants had similar homolog distributions and congener profiles to the PCNs emitted from combustion plants and other metallurgical processes. The identification and preliminary evaluation of PCN emissions from HDG plants presented here will help in the prioritization of measures for controlling PCN emissions from industrial sources.

  4. CH2M Hill Hanford Group, Inc. Standards and Requirements Identification Document (SRID) Requirements Management System and Requirements Specification

    Energy Technology Data Exchange (ETDEWEB)

    JOHNSON, A.L.

    2000-11-30

    The current Tank Farm Contractor (TFC) for the U. S. Department of Energy, Office of River Protection (ORP), River Protection Project (RPP), CH2M Hill Hanford Group, Inc. (CHG), will use a computer based requirements management system. The system will serve as a tool to assist in identifying, capturing, and maintaining the Standards/Requirements Identification Document (S/RID) requirements and links to implementing procedures and other documents. By managing requirements as one integrated set, CHG will be able to carry out its mission more efficiently and effectively. CHG has chosen the Dynamic Object Oriented Requirements System (DOORS{trademark}) as the preferred computer based requirements management system. Accordingly, the S/RID program will use DOORS{trademark}. DOORS{trademark} will replace the Environmental Requirements Management Interface (ERMI) system as the tool for S/RID data management. The DOORS{trademark} S/RID test project currently resides on the DOORSTM test server. The S/RID project will be migrated to the DOORS{trademark} production server. After the migration the S/RID project will be considered a production project and will no longer reside on the test server.

  5. ITS as a data resource: Preliminary requirements for a user service

    Energy Technology Data Exchange (ETDEWEB)

    Margiotta, R.

    1998-04-01

    Contents of this report are: Executive Summary; Introduction; The Need for an Archived Data User Service; Technical and Institutional Issues for Implementation; Basic Requirements; Appendix A: Summary of Recent Data Needs Identification Efforts; Appendix B: Supplemental Information on the Long-Term Pavement Performance Traffic Data Structure.

  6. Standards/requirements identification documents (S/RIDS)

    Energy Technology Data Exchange (ETDEWEB)

    Beckman, W.H.; Alhadeff, N.

    1994-09-01

    This paper describes the Fernald Environmental Restoration Management Corporation`s (FERMCO) Standards/Requirement Identification Documents (S/RIDs) Development Program, the unique process used to implement it, and the status of the program. We will also discuss the lessons learned as the development program was implemented. The Department of Energy (DOE) established the Fernald site to produce uranium metals for the nation`s defense programs in 1953. In 1989, DOE suspended production and, in 1991, the mission of the site was formally changed to one of environmental cleanup and restoration. The site was renamed the Fernald Environmental Management Project (FEMP) to reflect this change. From its inception until November 1992, the site was managed under a Management and Operating contract. As a result in the change in mission, DOE awarded an Environmental Restoration Management Contract (ERMC), focusing on restoration. FERMCO assumed management of the site December 1, 1992. The joint DOE/FERMCO mission is to protect human health and the environment through the safe, early, and least-cost final clean-up of the site in compliance with all applicable regulations and commitments while addressing stakeholder concerns. DOE has managed nuclear facilities primarily through its oversight of Management and Operating contractors. These contractors were responsible for formulating, selecting, and administering standards controlling design, construction, operations, and maintenance. The DOE Operations Office Manager was responsible for approving individual contractor practices and the governing site standards and requirements to be met. Due to the absence of comprehensive nuclear industry standards when most DOE sites were first established, Management and Operating contractors had to apply existing non-nuclear industry standards and, in many cases, formulate new technical standards to address unique applications.

  7. Purification, identification and preliminary crystallographic studies of Pru du amandin, an allergenic protein from Prunus dulcis

    Energy Technology Data Exchange (ETDEWEB)

    Gaur, Vineet; Sethi, Dhruv K.; Salunke, Dinakar M., E-mail: dinakar@nii.res.in [National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067 (India)

    2008-01-01

    The purification, identification, crystallization and preliminary crystallographic studies of an allergy-related protein, Pru du amandin, from P. dulcis nuts are reported. Food allergies appear to be one of the foremost causes of hypersensitivity reactions. Nut allergies account for most food allergies and are often permanent. The 360 kDa hexameric protein Pru du amandin, a known allergen, was purified from almonds (Prunus dulcis) by ammonium sulfate fractionation and ion-exchange chromatography. The protein was identified by a BLAST homology search against the nonredundant sequence database. Pru du amandin belongs to the 11S legumin family of seed storage proteins characterized by the presence of a cupin motif. Crystals were obtained by the hanging-drop vapour-diffusion method. The crystals belong to space group P4{sub 1} (or P4{sub 3}), with unit-cell parameters a = b = 150.7, c = 164.9 Å.

  8. Early Engagement of Safety and Mission Assurance Expertise Using Systems Engineering Tools: A Risk-Based Approach to Early Identification of Safety and Assurance Requirements

    Science.gov (United States)

    Darpel, Scott; Beckman, Sean

    2016-01-01

    Decades of systems engineering practice have demonstrated that the earlier the identification of requirements occurs, the lower the chance that costly redesigns will needed later in the project life cycle. A better understanding of all requirements can also improve the likelihood of a design's success. Significant effort has been put into developing tools and practices that facilitate requirements determination, including those that are part of the model-based systems engineering (MBSE) paradigm. These efforts have yielded improvements in requirements definition, but have thus far focused on a design's performance needs. The identification of safety & mission assurance (S&MA) related requirements, in comparison, can occur after preliminary designs are already established, yielding forced redesigns. Engaging S&MA expertise at an earlier stage, facilitated by the use of MBSE tools, and focused on actual project risk, can yield the same type of design life cycle improvements that have been realized in technical and performance requirements.

  9. Basic requirements for a preliminary conceptual design of the Korea advanced pyroprocess facility (KAPF)

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ho Hee; Ko, Won Il; Chang, Hong Lae; Song, Dae Yong; Kwon, Eun Ha; Lee, Jung Won [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2008-12-15

    Korea Atomic Energy Research Institute (KAERI) has been developing technologies for pyroprocessing for spent PWR fuels. This study is part of a long term R and D program in Korea to develop an advanced recycle system that has the potential to meet and exceed the proliferation resistance, waste minimization, resource minimization, safety and economic goals of approved Korean Government energy policy, as well as the Generation IV International Forum (GIF) program. To support this R and D program, KAERI requires that an independent estimate be made of the conceptual design and cost for construction and operation of a 'Korea Advanced Pyroprocessing Facility', This document describes the basic requirements for preliminary conceptual design of the Korea Advanced Pyroprocess Facility (KAPF). The presented requirements will be modified to be more effective and feasible on an engineering basis during the subsequent design process.

  10. 10 CFR 707.7 - Random drug testing requirements and identification of testing designated positions.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Random drug testing requirements and identification of... PROGRAMS AT DOE SITES Procedures § 707.7 Random drug testing requirements and identification of testing... evidence of the use of illegal drugs of employees in testing designated positions identified in this...

  11. 32 CFR 1901.13 - Requirements as to identification of requester.

    Science.gov (United States)

    2010-07-01

    ... the minor's birth certificate, court order, or representational agreement which establishes the... 32 National Defense 6 2010-07-01 2010-07-01 false Requirements as to identification of requester... Requirements as to identification of requester. (a) In general. Individuals seeking access to or amendment...

  12. 32 CFR 1801.13 - Requirements as to identification of requester.

    Science.gov (United States)

    2010-07-01

    ... birth certificate, court order, or representational agreement which establishes the relationship and the... 32 National Defense 6 2010-07-01 2010-07-01 false Requirements as to identification of requester... Requirements as to identification of requester. (a) In general. Individuals seeking access to or amendment...

  13. Software requirements flow-down and preliminary software design for the G-CLEF spectrograph

    Science.gov (United States)

    Evans, Ian N.; Budynkiewicz, Jamie A.; DePonte Evans, Janet; Miller, Joseph B.; Onyuksel, Cem; Paxson, Charles; Plummer, David A.

    2016-08-01

    The Giant Magellan Telescope (GMT)-Consortium Large Earth Finder (G-CLEF) is a fiber-fed, precision radial velocity (PRV) optical echelle spectrograph that will be the first light instrument on the GMT. The G-CLEF instrument device control subsystem (IDCS) provides software control of the instrument hardware, including the active feedback loops that are required to meet the G-CLEF PRV stability requirements. The IDCS is also tasked with providing operational support packages that include data reduction pipelines and proposal preparation tools. A formal, but ultimately pragmatic approach is being used to establish a complete and correct set of requirements for both the G-CLEF device control and operational support packages. The device control packages must integrate tightly with the state-machine driven software and controls reference architecture designed by the GMT Organization. A model-based systems engineering methodology is being used to develop a preliminary design that meets these requirements. Through this process we have identified some lessons that have general applicability to the development of software for ground-based instrumentation. For example, tasking an individual with overall responsibility for science/software/hardware integration is a key step to ensuring effective integration between these elements. An operational concept document that includes detailed routine and non- routine operational sequences should be prepared in parallel with the hardware design process to tie together these elements and identify any gaps. Appropriate time-phasing of the hardware and software design phases is important, but revisions to driving requirements that impact software requirements and preliminary design are inevitable. Such revisions must be carefully managed to ensure efficient use of resources.

  14. Development of a preliminary PIRT (Phenomena Identification and Ranking Table) of thermal-hydraulic phenomena for SMART

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Bub Dong; Lee, Won Jae; Kim, Hee Cheol; Song, Jin Ho; Sim, Suk Ku [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1997-12-31

    The work reported in this paper identifies the thermal-hydraulic phenomena that are expected to occur during a number of key transients in SMART (System-integrated Modular Advanced ReacTor) which is under development at KAERI. The result of this effort is based on the current design concept of SMART integral reactor. Although the design is still evolving, the preliminary Phenomena Identification and Ranking Table (PIRT) has been developed based on the experts` knowledge and experience. The preliminary PIRT has been developed by consensus of KAERI expert panelists and AHP (Analytical Hierarchy Process). Preliminary PIRT developed in this paper is intended to be used to identify and integrate development areas of further experimental tests needed, thermal hydraulic models and correlations and code improvements for the safety analysis of the SMART. 8 refs., 4 tabs (Author)

  15. Thirty Meter Telescope: observatory software requirements, architecture, and preliminary implementation strategies

    Science.gov (United States)

    Silva, David R.; Angeli, George; Boyer, Corinne; Sirota, Mark; Trinh, Thang

    2008-07-01

    The Thirty Meter Telescope (TMT) will be a ground-based, 30-m optical-IR alt-az telescope with a highly segmented primary mirror located in a remote location. Efficient science operations require the asynchronous coordination of many different sub-systems including telescope mount, three independent active optics sub-systems, adaptive optics, laser guide stars, and user-configured science instrument. An important high-level requirement is target acquisition and observatory system configuration must be completed in less than 5 minutes (or 10 minutes if moving to a new instrument). To meet this coordination challenge and target acquisition time requirement, a distributed software architecture is envisioned consisting of software components linked by a service-based software communications backbone. A master sequencer coordinates the activities of mid-layer sequencers for the telescope, adaptive optics, and selected instrument. In turn, these mid-layer sequencers coordinate the activities of groups of sub-systems. In this paper, TMT observatory requirements are presented in more detail, followed by a description of the design reference software architecture and a discussion of preliminary implementation strategies.

  16. 41 CFR 101-27.206-2 - Identification and shipping requirements.

    Science.gov (United States)

    2010-07-01

    ...-INVENTORY MANAGEMENT 27.2-Management of Shelf-Life Materials § 101-27.206-2 Identification and shipping... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Identification and shipping requirements. 101-27.206-2 Section 101-27.206-2 Public Contracts and Property Management Federal...

  17. 78 FR 20558 - Transportation Worker Identification Credential (TWIC)-Reader Requirements

    Science.gov (United States)

    2013-04-05

    ... SECURITY Coast Guard 33 CFR 101,104,105,106 Transportation Worker Identification Credential (TWIC)--Reader... 22, 2013, under the title ``Transportation Worker Identification Credential (TWIC)--Reader... comments on the notice of proposed rulemaking on TWIC reader requirements. DATES: A public meeting will be...

  18. 78 FR 20289 - Transportation Worker Identification Credential (TWIC)-Reader Requirements

    Science.gov (United States)

    2013-04-04

    ... SECURITY Coast Guard 33 CFR 101,104,105,106 Transportation Worker Identification Credential (TWIC)--Reader... 22, 2013, under the title ``Transportation Worker Identification Credential (TWIC)--Reader... comments on the notice of proposed rulemaking on TWIC reader requirements. DATES: A public meeting will be...

  19. 40 CFR 80.521 - What are the standards and identification requirements for diesel fuel additives?

    Science.gov (United States)

    2010-07-01

    ... Motor Vehicle Diesel Fuel; Nonroad, Locomotive, and Marine Diesel Fuel; and ECA Marine Fuel Motor... consumer in diesel motor vehicles or nonroad diesel engines. ... identification requirements for diesel fuel additives? 80.521 Section 80.521 Protection of...

  20. Westinghouse Hanford Company (WHC) standards/requirements identification document (S/RID)

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, G.L.

    1996-03-15

    This Standards/Requirements Identification Document (S/RID) set forth the Environmental Safety and Health (ES&H) standards/requirements for Westinghouse Hanford Company Level Programs, where implementation and compliance is the responsibility of these organizations. These standards/requirements are adequate to ensure the protection of the health and safety of workers, the public, and the environment.

  1. High level waste storage tanks 242-A evaporator standards/requirement identification document

    Energy Technology Data Exchange (ETDEWEB)

    Biebesheimer, E.

    1996-01-01

    This document, the Standards/Requirements Identification Document (S/RIDS) for the subject facility, represents the necessary and sufficient requirements to provide an adequate level of protection of the worker, public health and safety, and the environment. It lists those source documents from which requirements were extracted, and those requirements documents considered, but from which no requirements where taken. Documents considered as source documents included State and Federal Regulations, DOE Orders, and DOE Standards

  2. Millimeter wave sensor requirements for maritime small craft identification

    Science.gov (United States)

    Krapels, Keith; Driggers, Ronald G.; Garcia, Jose; Boettcher, Evelyn; Prather, Dennis; Schuetz, Chrisopher; Samluk, Jesse; Stein, Lee; Kiser, William; Visnansky, Andrew; Grata, Jeremy; Wikner, David; Harris, Russ

    2009-09-01

    Passive millimeter wave (mmW) imagers have improved in terms of resolution sensitivity and frame rate. Currently, the Office of Naval Research (ONR), along with the US Army Research, Development and Engineering Command, Communications Electronics Research Development and Engineering Center (RDECOM CERDEC) Night Vision and Electronic Sensor Directorate (NVESD), are investigating the current state-of-the-art of mmW imaging systems. The focus of this study was the performance of mmW imaging systems for the task of small watercraft / boat identification field performance. First mmW signatures were collected. This consisted of a set of eight small watercrafts; at 5 different aspects, during the daylight hours over a 48 hour period in the spring of 2008. Target characteristics were measured and characteristic dimension, signatures, and Root Sum Squared of Target's Temperature (RRSΔT) tabulated. Then an eight-alternative, forced choice (8AFC) human perception experiment was developed and conducted at NVESD. The ability of observers to discriminate between small watercraft was quantified. Next, the task difficulty criterion, V50, was quantified by applying this data to NVESD's target acquisition models using the Targeting Task Performance (TTP) metric. These parameters can be used to evaluate sensor field performance for Anti-Terrorism / Force Protection (AT/FP) and navigation tasks for the U.S. Navy, as well as for design and evaluation of imaging passive mmW sensors for both the U.S. Navy and U.S. Coast Guard.

  3. Identification and Comparison of Requirements Placed on Brand Managers

    Directory of Open Access Journals (Sweden)

    Zuzana Wroblowská

    2016-09-01

    Full Text Available The purpose of the article: Building a strong brand is difficult, even when viewing brands as an essential asset of the company is a lasting trend. So for brand managers to succeed in their job roles, they must possess knowledge, skills, and relevant personality traits. The purpose of the author´s work was to provide the insight into the offering of brand manager´s posts in the Czech Republic and to analyze the collected data with the special interest in finding the answer, whether the employers see a brand manager as a knowledge worker. Methodology/methods: For a successful empirical research, the content analysis method by Berelson was used to study the texts in recruitment advertisements. The statistical verification of results was conducted using the chi-squared test. Scientific aim: The goal of the primary survey was to provide answers to the research questions, which arise from the following research assumption: (1 the set of requirements for candidates for the job of Brand Manager will confirm that a brand manager is one of the workers who have skills and personality prerequisites expected for knowledge workers and (2 after the period of economic recession and despite the oncoming recovery, employers do not concede their requirements placed on candidates for the job of Brand Manager. Findings: The results of the actual research project confirmed a fundamental need of excellent communication skills (69.6 %, a high degree of creativity (51.8 % and analytical ability (42.9 % of candidates for the position “Brand Manager”. During the comparison of the data results gathered in 2007 there were recorded some differences. Statistical verification confirmed the shift of requirements in the group of traits and skills connected to motivation and self-control. The results also showed significant differences in the analysed group of ads for the position of “Brand Manager” and “Product Manager” in almost all groups of psychological

  4. Identification of Drosophila Gene Products Required for Phagocytosis of Candidaalbicans.

    Directory of Open Access Journals (Sweden)

    2005-12-01

    Full Text Available Phagocytosis is a highly conserved aspect of innate immunity. We used Drosophila melanogaster S2 cells as a model system to study the phagocytosis of Candida albicans, the major fungal pathogen of humans, by screening an RNAi library representing 7,216 fly genes conserved among metazoans. After rescreening the initial genes identified and eliminating certain classes of housekeeping genes, we identified 184 genes required for efficient phagocytosis of C. albicans. Diverse biological processes are represented, with actin cytoskeleton regulation, vesicle transport, signaling, and transcriptional regulation being prominent. Secondary screens using Escherichia coli and latex beads revealed several genes specific for C. albicans phagocytosis. Characterization of one of those gene products, Macroglobulin complement related (Mcr, shows that it is secreted, that it binds specifically to the surface of C. albicans, and that it promotes its subsequent phagocytosis. Mcr is closely related to the four Drosophila thioester proteins (Teps, and we show that TepII is required for efficient phagocytosis of E. coli (but not C. albicans or Staphylococcus aureus and that TepIII is required for the efficient phagocytosis of S. aureus (but not C. albicans or E. coli. Thus, this family of fly proteins distinguishes different pathogens for subsequent phagocytosis.

  5. Identification of patient's requirements in quality management system in health care institutions.

    Science.gov (United States)

    Kaźmierczak, Daniel; Bogusz-Czerniewicz, Marta

    2011-12-16

    To present the solutions implemented in health care institution in the context of identification of patient's requirements, and evaluation of the level of patients' satisfaction in accordance with the requirements of ISO norm 9001:2008 based on the experience of GPCC. The fundamental mechanisms behind the free market, such as competition, start applying also to the public health sector. Health service providers are gradually realising that patients are actual clients of health care institutions, with physicians, nurses, supporting personnel, registration officers and other staff responding to patients demand for medical and auxiliary services (e.g. exam registration, provision of information). PN-EN ISO 9001:2009 "Quality Management Systems. Requirements", relevant literature and documentation of quality management system from the GPCC. The review of relevant literature and legal requirements; interpretation of provisions in relation to the functioning of health care institutions. Model of identification of patient's requirements and satisfaction in accordance with the requirements of ISO 9001:2008 has been elaborated and implemented in the GPCC. The identification of patient's requirements is much more complicated than evaluating the same parameters in manufacturing companies. In the context of medical services one should be aware of the subjectivity of patient's feelings, the psycho-social status and the general state of health during his or her treatment. Therefore, the identification of patient's requirements and satisfaction must be carefully thought out, implemented and regularly improved.

  6. 78 FR 18534 - Transportation Worker Identification Credential (TWIC)-Reader Requirements

    Science.gov (United States)

    2013-03-27

    ... (TWIC)--Reader Requirements AGENCY: Coast Guard, DHS. ACTION: Notice of public meeting; request for... 22, 2013, under the title ``Transportation Worker Identification Credential (TWIC)--Reader... comments on the notice of proposed rulemaking on TWIC reader requirements. DATES: A public meeting will be...

  7. 78 FR 22218 - Transportation Worker Identification Credential (TWIC)-Reader Requirements

    Science.gov (United States)

    2013-04-15

    ...)--Reader Requirements AGENCY: Coast Guard, DHS. ACTION: Notice of public meeting on proposed rulemaking... on March 22, 2013, under the title ``Transportation Worker Identification Credential (TWIC)--Reader... comments on the notice of proposed rulemaking on TWIC reader requirements. DATES: A public meeting will be...

  8. C2 Link Security for UAS: Technical Literature Study and Preliminary Functional Requirements. Version 0.9 (Working Draft)

    Science.gov (United States)

    2005-01-01

    This document provides a study of the technical literature related to Command and Control (C2) link security for Unmanned Aircraft Systems (UAS) for operation in the National Airspace System (NAS). Included is a preliminary set of functional requirements for C2 link security.

  9. The Habitable Exoplanet (HabEx) Imaging Mission: preliminary science drivers and technical requirements

    Science.gov (United States)

    Mennesson, Bertrand; Gaudi, Scott; Seager, Sara; Cahoy, Kerri; Domagal-Goldman, Shawn; Feinberg, Lee; Guyon, Olivier; Kasdin, Jeremy; Marois, Christian; Mawet, Dimitri; Tamura, Motohide; Mouillet, David; Prusti, Timo; Quirrenbach, Andreas; Robinson, Tyler; Rogers, Leslie; Scowen, Paul; Somerville, Rachel; Stapelfeldt, Karl; Stern, Daniel; Still, Martin; Turnbull, Margaret; Booth, Jeffrey; Kiessling, Alina; Kuan, Gary; Warfield, Keith

    2016-07-01

    HabEx is one of four candidate flagship missions being studied in detail by NASA, to be submitted for consideration to the 2020 Decadal Survey in Astronomy and Astrophysics for possible launch in the 2030s. It will be optimized for direct imaging and spectroscopy of potentially habitable exoplanets, and will also enable a wide range of general astrophysics science. HabEx aims to fully characterize planetary systems around nearby solar-type stars for the first time, including rocky planets, possible water worlds, gas giants, ice giants, and faint circumstellar debris disks. In particular, it will explore our nearest neighbors and search for signs of habitability and biosignatures in the atmospheres of rocky planets in the habitable zones of their parent stars. Such high spatial resolution, high contrast observations require a large (roughly greater than 3.5m), stable, and diffraction-limited optical space telescope. Such a telescope also opens up unique capabilities for studying the formation and evolution of stars and galaxies. We present some preliminary science objectives identified for HabEx by our Science and Technology Definition Team (STDT), together with a first look at the key challenges and design trades ahead.

  10. The Habitable Exoplanet (HabEx) Imaging Mission: Preliminary Science Drivers and Technical Requirements

    Science.gov (United States)

    Gaudi, B. Scott; Habitable Exoplanet Imaging Mission Science and Technology Definition Team

    2017-01-01

    HabEx is one of four candidate flagship missions being studied in detail by NASA, to be submitted for consideration to the 2020 Decadal Survey in Astronomy and Astrophysics for possible launch in the 2030s. It will be optimized for direct imaging and spectroscopy of potentially habitable exoplanets, and will also enable a wide range of general astrophysics science. HabEx aims to fully characterize planetary systems around nearby solar-type stars for the first time, including rocky planets, possible water worlds, gas giants, ice giants, and faint circumstellar debris disks. In particular, it will explore our nearest neighbors and search for signs of habitability and biosignatures in the atmospheres of rocky planets in the habitable zones of their parent stars. Such high spatial resolution, high contrast observations require a large (roughly greater than 3.5m), stable, and diffraction-limited optical space telescope. Such a telescope also opens up unique capabilities for studying the formation and evolution of stars and galaxies. We present some preliminary science objectives identified for HabEx by our Science and Technology Definition Team (STDT), together with a first look at the key challenges and design trades ahead.

  11. Plutonium Finishing Plant (PFP) Standards/Requirements Identification Document (S/RID)

    Energy Technology Data Exchange (ETDEWEB)

    Maddox, B.S.

    1996-01-01

    This Standards/Requirements Identification Document (S/RID) sets forth the Environmental Safety and Health (ESH) standards/requirements for the Plutonium Finishing Plant (PFP). This S/RID is applicable to the appropriate life cycle phases of design, construction, operation, and preparation for decommissioning. These standards/requirements are adequate to ensure the protection of the health and safety of workers, the public, and the environment.

  12. Waste encapsulation storage facility (WESF) standards/requirements identification document (S/RIDS)

    Energy Technology Data Exchange (ETDEWEB)

    Maddox, B.S., Westinghouse Hanford

    1996-07-29

    This Standards/Requirements Identification Document (S/RID) sets forth the Environmental Safety and Health (ES{ampersand}H) standards/requirements for the Waste Encapsulation Storage Facility (WESF). This S/RID is applicable to the appropriate life cycle phases of design, construction, operation, and preparation for decommissioning. These standards/requirements are adequate to ensure the protection of the health and safety of workers, the public, and the environment.

  13. Preliminary evaluation of alternative waste form solidification processes. Volume I. Identification of the processes.

    Energy Technology Data Exchange (ETDEWEB)

    Treat, R.L.; Nesbitt, J.F.; Blair, H.T.; Carter, J.G.; Gorton, P.S.; Partain, W.L.; Timmerman, C.L.

    1980-04-01

    This document contains preconceptual design data on 11 processes for the solidification and isolation of nuclear high-level liquid wastes (HLLW). The processes are: in-can glass melting (ICGM) process, joule-heated glass melting (JHGM) process, glass-ceramic (GC) process, marbles-in-lead (MIL) matrix process, supercalcine pellets-in-metal (SCPIM) matrix process, pyrolytic-carbon coated pellets-in-metal (PCCPIM) matrix process, supercalcine hot-isostatic-pressing (SCHIP) process, SYNROC hot-isostatic-pressing (SYNROC HIP) process, titanate process, concrete process, and cermet process. For the purposes of this study, it was assumed that each of the solidification processes is capable of handling similar amounts of HLLW generated in a production-sized fuel reprocessing plant. It was also assumed that each of the processes would be enclosed in a shielded canyon or cells within a waste facility located at the fuel reprocessing plant. Finally, it was assumed that all of the processes would be subject to the same set of regulations, codes and standards. Each of the solidification processes converts waste into forms that may be acceptable for geological disposal. Each process begins with the receipt of HLLW from the fuel reprocessing plant. In this study, it was assumed that the original composition of the HLLW would be the same for each process. The process ends when the different waste forms are enclosed in canisters or containers that are acceptable for interim storage. Overviews of each of the 11 processes and the bases used for their identification are presented in the first part of this report. Each process, including its equipment and its requirements, is covered in more detail in Appendices A through K. Pertinent information on the current state of the art and the research and development required for the implementation of each process are also noted in the appendices.

  14. Preliminary identification schemes for some unicellular ciliated and flagellated parasites of warmwater fishes

    Science.gov (United States)

    There are few keys for the identification of small unicellular parasites of warmwater fishes and few experts who can confidently identify these parasites to species. Molecular identification tools for these parasites are largely unavailable. For fishery biologists and even fish health diagnosticia...

  15. Preliminary studies on identification of inorganic species in complex mixtures by electrospray mass spectrometry in the counter ion mode

    Energy Technology Data Exchange (ETDEWEB)

    Mollah, Sahana

    1999-11-08

    Suppression of mass spectral peaks due to matrix problem is a major hurdle to overcome during identification work. So far, preliminary studies have been done in investigating solutions containing various percentages of nitric and hydrochloric acid. Since other anions would also be present in real samples, also needed to be examined is how the extent of suppression of metal complexes by Cl{sup {minus}} compares with suppression by other anions such as PO{sub 4}{sup 3{minus}} or SO{sub 4}{sup 2{minus}}. If suppression of other anions is as severe as that of the chloride ion, then it would be virtually impossible to analyze unknown samples containing large amount of such anions by direct infusion electrospray mass spectrometry. It seems like a separation step is needed to separate these matrix anions from the metal complexes prior to putting the solution through the electrospray. However, separation of inorganic complexes can be difficult and has not been studied thoroughly as LC separation of bioorganic compounds. Both zinc and copper chloro complexes have been observed to be more tolerant to higher amount of chloride ion present in a solution compared to the group I and II metal chloro complexes. Other transition metals including the lanthanide complexes need to be examined more intensively to see how they fare against other transition metal complexes. So far, only preliminary work has been done in identifying inorganic species in solutions using both ICP-MS and ES-MS. The solution contained a number of metals but only one major anion, NO{sub 3}{sup {minus}}. Therefore, complex solutions containing a number of anions and metals can be examined to see if identification is still feasible. This identification work can be continued on into investigating real samples.

  16. 41 CFR 102-34.85 - What motor vehicles require motor vehicle identification?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What motor vehicles require motor vehicle identification? 102-34.85 Section 102-34.85 Public Contracts and Property Management... 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle...

  17. 75 FR 3416 - Fisheries in the Western Pacific; Pelagic Fisheries; Vessel Identification Requirements

    Science.gov (United States)

    2010-01-21

    ... National Oceanic and Atmospheric Administration 50 CFR Part 665 RIN 0648-AX38 Fisheries in the Western Pacific; Pelagic Fisheries; Vessel Identification Requirements AGENCY: National Marine Fisheries Service... INFORMATION CONTACT: Jarad Makaiau, Sustainable Fisheries, NMFS PIR, 808-944-2108. SUPPLEMENTARY...

  18. Identification of Genes Required for the Survival of BRCA 1-/- Cells

    Science.gov (United States)

    2010-02-01

    homologous recombination26, 27. The numerous functions of BRCA1 complexes highlight the importance of this protein in normal cellular function, and the ways...to double-strand breaks. Science 286, 1162-6 (1999). 36. Smogorzewska, A. et al. Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair. Cell 129, 289-301 (2007).

  19. 16 CFR 1014.4 - Requirements for identification of individuals making requests.

    Science.gov (United States)

    2010-01-01

    ... may establish his or her identity by the presentation of a single document bearing a photograph (such... identification of individuals making requests. The following proof of identity is required for requests for... of his or her identity may provide a written statement affirming his or her identity and the...

  20. Required Preliminary Administrative Service Credential Program Culminating Activities in California NCATE Accredited Universities

    Science.gov (United States)

    Wildman, Louis

    2014-01-01

    The purpose of this effort is to share information about the variety of culminating activities used in the acquisition of the California Preliminary Administrative Services Credential. Knowledge of these varying culminating activities and related practices has not previously been readily available. The culminating activities among California's…

  1. Architecturally Significant Requirements Identification, Classification and Change Management for Multi-tenant Cloud-Based Systems

    DEFF Research Database (Denmark)

    Chauhan, Muhammad Aufeef; Probst, Christian W.

    2017-01-01

    identification, classification, and change management challenges. We have explored findings from systematic as well as structured reviews of the literature on quality requirements of the cloud-based systems including but not limited to security, availability, scalability, privacy, and multi-tenancy. We have...... applicability of the framework on a selected set of the requirements for the cloud-based systems. The results of the research presented in this chapter show that key quality requirements of the cloud-based systems, for example, multi-tenancy and security, have a significant impact on how other quality...

  2. Identification and preliminary SAR studies of (+)-Geodin as a glucose uptake stimulator for rat adipocytes.

    Science.gov (United States)

    Sato, Seiichi; Okusa, Noriyuki; Ogawa, Akiyo; Ikenoue, Takao; Seki, Tetsuya; Tsuji, Takashi

    2005-09-01

    (+)-Geodin (1) was isolated from Penicillium glabrum AJ117540 with activity that stimulates glucose uptake by rat adipocytes. Unlike insulin it is active in the presence of wortmannin. Dihydrogeodin (2) and sulochrin (3) which are the precursors of (+)-geodin biosynthesis were also isolated from the same fungus. Preliminary SAR studies of 1 showed some analogues had enhanced activity. Especially, the activities of racemic geodin and dibromo analogue (7a) were comparable to that of the natural product. Geodin (1), a known fungal metabolite, was isolated from Penicillium glabrum AJ117540 as an active substance (Fig. 1). Dihydrogeodin (2) and sulochrin (3), the precursors of 1, were also isolated from the same fungal extract. In this study, preliminary mechanistic insight and SAR are reported.

  3. 50 CFR 697.21 - Gear identification and marking, escape vent, maximum trap size, and ghost panel requirements.

    Science.gov (United States)

    2010-10-01

    ... vent, maximum trap size, and ghost panel requirements. 697.21 Section 697.21 Wildlife and Fisheries... identification and marking, escape vent, maximum trap size, and ghost panel requirements. (a) Gear identification... Administrator finds to be consistent with paragraph (c) of this section. (d) Ghost panel. (1) Lobster traps...

  4. NGS meta data analysis for identification of SNP and INDEL patterns in human airway transcriptome: A preliminary indicator for lung cancer

    Directory of Open Access Journals (Sweden)

    Sathya B.

    2015-03-01

    Full Text Available High-throughput sequencing of RNA (RNA-Seq was developed primarily to analyze global gene expression in different tissues. It is also an efficient way to discover coding SNPs and when multiple individuals with different genetic backgrounds were used, RNA-Seq is very effective for the identification of SNPs. The objective of this study was to perform SNP and INDEL discoveries in human airway transcriptome of healthy never smokers, healthy current smokers, smokers without lung cancer and smokers with lung cancer. By preliminary comparative analysis of these four data sets, it is expected to get SNP and INDEL patterns responsible for lung cancer. A total of 85,028 SNPs and 5738 INDELs in healthy never smokers, 32,671 SNPs and 1561 INDELs in healthy current smokers, 50,205 SNPs and 3008 INDELs in smokers without lung cancer and 51,299 SNPs and 3138 INDELs in smokers with lung cancer were identified. The analysis of the SNPs and INDELs in genes that were reported earlier as differentially expressed was also performed. It has been found that a smoking person has SNPs at position 62,186,542 and 62,190,293 in SCGB1A1 gene and 180,017,251, 180,017,252, and 180,017,597 in SCGB3A1 gene and INDELs at position 35,871,168 in NFKBIA gene and 180,017,797 in SCGB3A1 gene. The SNPs identified in this study provides a resource for genetic studies in smokers and shall contribute to the development of a personalized medicine. This study is only a preliminary kind and more vigorous data analysis and wet lab validation are required.

  5. Identification of preliminary core outcome domains for communication about childhood vaccination: An online Delphi survey.

    Science.gov (United States)

    Kaufman, Jessica; Ryan, Rebecca; Lewin, Simon; Bosch-Capblanch, Xavier; Glenton, Claire; Cliff, Julie; Oyo-Ita, Angela; Muloliwa, Artur Manuel; Oku, Afiong; Ames, Heather; Rada, Gabriel; Cartier, Yuri; Hill, Sophie

    2017-08-20

    Communication interventions for childhood vaccination are promising strategies to address vaccine hesitancy, but current research is limited by the outcomes measured. Most studies measure only vaccination-related outcomes, with minimal consideration of vaccine hesitancy-relevant intermediate outcomes. This impedes understanding of which interventions or elements are effective. It is also unknown which outcomes are important to the range of stakeholders affected by vaccine hesitancy. Outcome selection shapes the evidence base, informing future interventions and trials, and should reflect stakeholder priorities. Therefore, our aim was to identify which outcome domains (i.e. broad outcome categories) are most important to different stakeholders, identifying preliminary core outcome domains to inform evaluation of three common vaccination communication types: (i) communication to inform or educate, (ii) remind or recall, and (iii) enhance community ownership. We conducted a two-stage online Delphi survey, involving four stakeholder groups: parents or community members, healthcare providers, researchers, and government or non-governmental organisation representatives. Participants rated the importance of eight outcome domains for each of the three communication types. They also rated specific outcomes within one domain ("attitudes or beliefs") and provided feedback about the survey. Collectively, stakeholder groups prioritised outcome domains differently when considering the effects of different communication types. For communication that aims to (i) inform or educate, the most important outcome domain is "knowledge or understanding"; for (ii) reminder communication, "vaccination status and behaviours"; and for (iii) community engagement communication, "community participation". All stakeholder groups rated most outcome domains as very important or critical. The highest rated specific outcome within the "attitudes or beliefs" domain was "trust". This Delphi survey

  6. Exopolysaccharide Production by Four Cyanobacterial Isolates and Preliminary Identification of These Isolates

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yunyi; CHI Zhenming; LU Weidong

    2007-01-01

    Four marine cyanobacterial isolates, named 104, 109, 113 and 115, from marine water off China's coast can release a large amount of exopolysaccharide (EPS) to medium. The effects of different components in medium on EPS production by the four isolates were investigated. Under the optimal condition, the EPS released by isolates 104,109, 113 and 115 reached 7.48 g L-1, 8.33 g L-1, 18.26 g L-1, and 6.78 g L-1 within 14 d,respectively. Based on the conventional identification methods for cyanobacteria,these isolates were assigned to genus Cyanothece.

  7. The design of an insulin pump - preliminary requirements (a technical note)

    Science.gov (United States)

    Hawlas, Hubert J.; Lewenstein, Krzysztof

    2009-01-01

    The material presented in this paper is an attempt to lay down requirements for the planned design of an insulin pump. An insulin pump is a device for continuous dosage of insulin at a selected rate, which facilitates treatment and improves the lives of diabetic patients. This paper is a compilation of medical requirements and user suggestions of presently offered insulin pumps. It seems important to establish proper requirements for a device before starting developing any design for an insulin pump.

  8. A preliminary study of the effect of eliminating requirements on clinical performance.

    Science.gov (United States)

    Dodge, W W; Dale, R A; Hendricson, W D

    1993-09-01

    This study determined the effect of a clinical program driven by patient needs upon students' productivity, attitudes, and academic performance. A group of eight senior students, whose academic and clinical performance profile replicated that of the rest of the class, were chosen to participate in a year-long non-requirement clinic. The students were expected to attend all clinic sessions, and treat their assigned patients. Their performance was compared to that of classmates in the regular requirement-driven curriculum. The non-requirement group had significantly higher academic achievement and significantly outproduced their classmates. Non-requirement students had no state board failures, versus 17 percent in the regular curriculum, and reported significantly lower stress. This study suggests that predoctoral clinical programs can maintain quality and productivity in the absence of unit requirements.

  9. Preliminary Results of Ocular Artefacts Identification in EEC Series by Neural Network

    Directory of Open Access Journals (Sweden)

    M. Kofronova

    1996-06-01

    Full Text Available The human electroencephalogram (EEG, is record of the electrical activity of the brain and contains useful diagnostic information on a variety of neurological disorders. Normal EEG signal are usually registered from electrodes placed on the scalp, and are often very small in amplitude, of 20 µV. The EEG, like all biomedical signals, is very susceptible to a variety of large signal contamination or artefacts (signals of other than brain activity which reduce its clinical usefulness. For example, blinking or moving eyes produces large electrical potentials around the eyes called the electrooculogram (EOG. The EOG spreads across the scalp to contaminate the EEG, when it is referred to as an ocular artefact (OA. This paper includes method of identification portion of the EEG record where ocular artefact appears and classification its type by neural network.

  10. Preliminary identification of fullerenes in the lowermost Jurassic strata, Queen Charlotte Islands, British Columbia

    Science.gov (United States)

    Perry, Randall S.; Haggart, James W.; Ward, Peter D.

    2004-02-01

    The Triassic-Jurassic (TJ) mass extinction (~200 mya) event is one of the most severe in geologic history. It is also one of the most poorly understood. Few geologic sections containing the TJ boundary interval have been identified globally, and most of those are poorly preserved; the paucity of suitable stratigraphic sections has prevented corroborative geochemical studies of this interval. Recently, fullerene molecules (C60 to C200) have been shown to be present in the mass extinction boundary intervals of the Permian-Triassic (PT) event (~251.4 mya), as well as the well-known "dinosaur" extinction event of the Cretaceous-Tertiary (KT) (~65 mya). The presence of fullerenes in both these extinction intervals has been used to invoke an extraterrestrial impact cause for the extinctions. Preliminary results of laser desorption mass spectrometry (LDMS) of selected samples from the Kennecott Point TJ boundary section, Queen Charlotte Islands, British Columbia, suggest that fullerenes (C60 to ~C200) are present in the section, stratigraphically above the extinction interval (as defined by paleontological and isotopic data), but not actually within the interval itself. The presence of fullerenes may not be diagnostic of an impact event.

  11. Development of a preliminary PIRT (Phenomena Identification and Ranking Table) of thermal-hydraulic phenomena for 330MWt SMART integral reactor

    Energy Technology Data Exchange (ETDEWEB)

    Chung, B. D.; Lee, W. J.; Sim, S. K.; Song, J. H.; Kim, H. C.

    1997-09-01

    The work reported in this document identifies the thermal-hydraulic phenomena that are expected to occur during a number of key transients in a 330 MWt SMART integral reactor which is under development at KAERI. The result of this efforts is based on the current design concept of SMART integral reactor. Although the design is still evolving, the preliminary Phenomena Identification and Ranking Table (PIRT) has been developed based on the experts` knowledge and experience. The preliminary PIRT has been developed by the consensus of KAERI expert panelists and AHP (Analytical Hierarchy Process). Preliminary PIRT developed in this report is intended for use to identify and integrate development areas of further experimental tests needed and thermal-hydraulic models and correlations and code improvements for the safety analysis of the SMART integral reactor. (author). 7 refs., 21 tabs., 22 figs.

  12. Subject-specific estimation of central aortic blood pressure via system identification: preliminary in-human experimental study.

    Science.gov (United States)

    Fazeli, Nima; Kim, Chang-Sei; Rashedi, Mohammad; Chappell, Alyssa; Wang, Shaohua; MacArthur, Roderick; McMurtry, M Sean; Finegan, Barry; Hahn, Jin-Oh

    2014-10-01

    This paper demonstrates preliminary in-human validity of a novel subject-specific approach to estimation of central aortic blood pressure (CABP) from peripheral circulatory waveforms. In this "Individualized Transfer Function" (ITF) approach, CABP is estimated in two steps. First, the circulatory dynamics of the cardiovascular system are determined via model-based system identification, in which an arterial tree model is characterized based on the circulatory waveform signals measured at the body's extremity locations. Second, CABP waveform is estimated by de-convolving peripheral circulatory waveforms from the arterial tree model. The validity of the ITF approach was demonstrated using experimental data collected from 13 cardiac surgery patients. Compared with the invasive peripheral blood pressure (BP) measurements, the ITF approach yielded significant reduction in errors associated with the estimation of CABP, including 1.9-2.6 mmHg (34-42 %) reduction in BP waveform errors (p < 0.05) as well as 5.8-9.1 mmHg (67-76 %) and 6.0-9.7 mmHg (78-85 %) reductions in systolic and pulse pressure (SP and PP) errors (p < 0.05). It also showed modest but significant improvement over the generalized transfer function approach, including 0.1 mmHg (2.6 %) reduction in BP waveform errors as well as 0.7 (20 %) and 5.0 mmHg (75 %) reductions in SP and PP errors (p < 0.05).

  13. Identification of macrophage extracellular trap-like structures in mammary gland adipose tissue: a preliminary study.

    Directory of Open Access Journals (Sweden)

    Sunish eMohanan

    2013-03-01

    Full Text Available PAD4-mediated hypercitrullination of histone H4 arginine 3 (H4R3 has been previously found to promote the formation of Neutrophil Extracellular Traps (NET in inflamed tissues and the resulting histone H4 citrulline 3 (H4Cit3 modification is thought to play a key role in extracellular trap (ET formation by promoting chromatin decondensation. In addition to neutrophils, macrophages have also recently been found to generate functional extracellular traps (METs. However, a role for PADs in ET formation in macrophages has not been previously described. Transcripts for PAD2 and PAD4 are found in mature macrophages and these cells can be induced to citrullinate proteins, thus raising the possibility that PADs may play a direct role in ET formation in macrophages via histone hypercitrullination. In breast and visceral white adipose tissue from obese patients, infiltrating macrophages are often seen to surround dead adipocytes forming characteristic crown-like structures (CLS and the presence of these lesions is associated with increased levels of inflammatory mediators. In light of these observations, we have initiated studies to test whether PADs are expressed in CLS macrophages and whether these macrophages might form METs. Our preliminary findings show that PAD2 (and to a lesser extent, PAD4 is expressed in both in the macrophage cell line (RAW 264.7 and in CLS lesions. Additionally, we provide evidence that macrophage-derived extracellular histones are seen around presumptive macrophages within CLS lesions and that these histones contain the H4Cit3 modification. These initial findings support our hypothesis that obesity-induced adipose tissue inflammation promotes the formation of METs within CLS lesions via PAD-mediated histone hypercitrullination. Subsequent studies are underway to further validate these findings and to investigate the role in PAD-mediated MET formation in CLS function in the mammary gland.

  14. Identification of succinic semialdehyde reductases from Geobacter: expression,purification, crystallization, preliminary functional, and crystallographic analysis

    Institute of Scientific and Technical Information of China (English)

    Yanfeng Zhang; Xiaoli Gao; Yi Zheng; R. Michae; Garavito

    2011-01-01

    Succinic semialdehyde reductase (SSAR) is an important enzyme involved in γ-aminobutyrate (GABA) metabolism.By converting succinic semialdehyde (SSA) to γ-hydroxybutyrate (GHB),the SSAR facilitates an alternative pathway for GABA degradation.In this study,we identified SSARs from Geobacter sulfurreducens and Geobacter metallireducens (GsSSAR and GmSSAR,respectively).The enzymes were over-expressed in Escherichia coil and purified to near homogeneity.Both GsSSAR and GmSSAR showed the activity of reducing SSA using nicotinamide adenine dinucleotide phosphate as a co-factor.The oligomeric sizes of GsSSAR and GmSSAR,as determined by analytical size exclusion chromatography,suggest that the enzymes presumably exist as tetramers in solution.The recombinant GsSSAR and GmSSAR crystallized in the presence of NADP+,and the resulting crystals diffracted to 1.89 (A) (GsSSAR) and 2.25 (A)(GmSSAR) resolution.The GsSSAR and GmSSAR crystals belong to the space groups P21221 (a =99.61 (A),b =147.49 (A),c =182.47 A) and P1 (a =75.97 (A) b =79.14 (A) c =95.47 (A),α =82.15°,β =88.80°,γ=87.66°),respectively.Preliminary crystallographic data analysis suggests the presence of eight protein monomers in the asymmetric units for both GsSSAR and GmSSAR.

  15. Identification of succinic semialdehyde reductases from Geobacter: expression, purification, crystallization, preliminary functional, and crystallographic analysis

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yanfeng; Gao, Xiaoli; Zheng, Yi; Garavito, R. Michael (MSU)

    2012-04-30

    Succinic semialdehyde reductase (SSAR) is an important enzyme involved in {gamma}-aminobutyrate (GABA) metabolism. By converting succinic semialdehyde (SSA) to {gamma}-hydroxybutyrate (GHB), the SSAR facilitates an alternative pathway for GABA degradation. In this study, we identified SSARs from Geobacter sulfurreducens and Geobacter metallireducens (GsSSAR and GmSSAR, respectively). The enzymes were over-expressed in Escherichia coli and purified to near homogeneity. Both GsSSAR and GmSSAR showed the activity of reducing SSA using nicotinamide adenine dinucleotide phosphate as a co-factor. The oligomeric sizes of GsSSAR and GmSSAR, as determined by analytical size exclusion chromatography, suggest that the enzymes presumably exist as tetramers in solution. The recombinant GsSSAR and GmSSAR crystallized in the presence of NADP{sup +}, and the resulting crystals diffracted to 1.89 {angstrom} (GsSSAR) and 2.25 {angstrom} (GmSSAR) resolution. The GsSSAR and GmSSAR crystals belong to the space groups P2{sub 1}22{sub 1} (a = 99.61 {angstrom}, b = 147.49 {angstrom}, c = 182.47 {angstrom}) and P1 (a = 75.97 {angstrom}, b = 79.14 {angstrom}, c = 95.47 {angstrom}, {alpha} = 82.15{sup o}, {beta} = 88.80{sup o}, {gamma} = 87.66{sup o}), respectively. Preliminary crystallographic data analysis suggests the presence of eight protein monomers in the asymmetric units for both GsSSAR and GmSSAR.

  16. Preliminary identification of secreted proteins by Leptospira interrogans serovar Kennewicki strain Pomona Fromm

    Energy Technology Data Exchange (ETDEWEB)

    Ricardi, L.M.P.; Portaro, F.C.; Abreu, P.A.E.; Barbosa, A.S. [Instituto Butantan, Sao Paulo, SP (Brazil); Morais, Z.M.; Vasconcellos, S.A. [Universidade de Sao Paulo (USP), SP (Brazil)

    2012-07-01

    Full text: This project aimed to identify secreted proteins by pathogenic Leptospira interrogans serovar Kennewicki strain Pomona Fromm (LPF) by proteomic analyses. The strain LPF, whose virulence was maintained by passages in hamsters, were cultured in EMJH medium. The supernatants were centrifuged, dialyzed and subjected to lyophilization. Protein samples were resolved first by IEF at pH 3 to 10, immobilized pH gradient 13-cm strips. Strips were then processed for the second-dimension separation on SDS-polyacrylamide gels. Proteins from gel spots were subjected to reduction, cysteine-alkylation, and in-gel tryptic digestion, and analyzed by LC/MS/MS spectrometry. Liquid chromatography-based separation followed by automated tandem mass spectrometry was also used to identify secreted proteins. In silico analyses were performed using the PSORTbV.3.0 program and SignalP server. One major obstacle to secretome studies is the difficulty to obtain extracts of secreted proteins without citoplasmatic contamination. In addition, the extraction of low concentration proteins from large volumes of culture media, which are rich in salts, BSA and other compounds, frequently interfere with most proteomics techniques. For these reasons, several experimental approaches were used to optimize the protocol applied. In spite of this fact, our analysis resulted in the identification of 200 proteins with high confidence. Only 5 of 63 secreted proteins predicted by in silico analysis were found. Other classes identified included proteins that possess signal peptide but whose cellular localization prediction is unknown or may have multiple localization sites, and proteins that lack signal peptide and are thus thought to be secreted via non conventional mechanisms or resulting from cytoplasmic contamination by cell lysis. Many of these are hypothetical proteins with no putative conserved domains detected. To our knowledge, this is the first study to identify secreted proteins by

  17. Preliminary findings on identification of mycorrhizal fungi from diverse orchids in the Central Highlands of Madagascar.

    Science.gov (United States)

    Yokoya, Kazutomo; Zettler, Lawrence W; Kendon, Jonathan P; Bidartondo, Martin I; Stice, Andrew L; Skarha, Shannon; Corey, Laura L; Knight, Audrey C; Sarasan, Viswambharan

    2015-11-01

    The Orchid flora of Madagascar is one of the most diverse with nearly 1000 orchid taxa, of which about 90% are endemic to this biodiversity hotspot. The Itremo Massif in the Central Highlands of Madagascar with a Highland Subtropical climate range encompasses montane grassland, igneous and metamorphic rock outcrops, and gallery and tapia forests. Our study focused on identifying culturable mycorrhizae from epiphytic, lithophytic, and terrestrial orchid taxa to understand their diversity and density in a spatial matrix that is within the protected areas. We have collected both juvenile and mature roots from 41 orchid taxa for isolating their orchid mycorrhizal fungi (OMF), and to culture, identify, and store in liquid nitrogen for future studies. Twelve operational taxonomic units (OTUs), of three known orchid mycorrhizal genera, were recognized by analysis of internal transcribed spacer (ITS) sequences of 85 isolates, and, by comparing with GenBank database entries, each OTU was shown to have closely related fungi that were also found as orchid associates. Orchid and fungal diversity were greater in gallery forests and open grasslands, which is very significant for future studies and orchid conservation. As far as we know, this is the first ever report of detailed identification of mycorrhizal fungi from Madagascar. This study will help start to develop a programme for identifying fungal symbionts from this unique biodiversity hotspot, which is undergoing rapid ecosystem damage and species loss. The diversity of culturable fungal associates, their density, and distribution within the Itremo orchid hotspot areas will be discussed.

  18. Preliminary study on the origin identification of natural gas by the parameters of light hydrocarbon

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The light hydrocarbon composition of 209 natural gas samples and individual light hydrocarbon carbon isotopes of 53 natural gas samples from typical humic-sourced gas and sapropelic-sourced gas in the four basins of China have been determined and analyzed.Some identification parameters for humic-sourced gas and sapropelic-sourced gas are proposed or corrected.The differences of compound-specificδ13C value of individual light hydrocarbon between humic-sourced gas and sapropelic-sourced gas have been founded.The humic-sourced gas has the distribution ofδ13C benzene > -24‰,δ13C toluene >-23‰,δ13C cyclohexane >-24‰andδ13C methyl cyclohexane >-24‰,while the sapropelicsourced gas has the distribution ofδ13C benzene <-24‰,δ13C toluene <-24‰,δ13C cyclohexane <-24‰and δ13C methyl cyclohexane <-24‰.Among the components of C7 light hydrocarbon compound,such as normal heptane(nC7),methyl cyclohexane(MCH)and dimethyl cyclopentane(ΣDMCP),etc,relative contents of nC7 and MCH are influenced mainly by the source organic matter type of natural gas.Therefore,it is suggested that the gas with relative content of nC7 of more than 30%and relative content of MCH of less than 70%is sapropelic-sourced gas,while gas with relative content of nC7 of less than 35%and relative content of MCH of more than 50%is humic-sourced gas.Among components of C5-7 aliphatics, the gas with relative content of C5-7 normal alkane of more than 30%is sapropelic-sourced gas,while the gas with relative content of C5-7 normal alkane of less than 30%is humic-sourced gas.These paremeters have been suggested to identify humic-sourced gas and sapropelic-sourced gas.

  19. Preliminary Assessment of Operational Hazards and Safety Requirements for Airborne Trajectory Management (ABTM) Roadmap Applications

    Science.gov (United States)

    Cotton, William B.; Hilb, Robert; Koczo, Stefan, Jr.; Wing, David J.

    2016-01-01

    A set of five developmental steps building from the NASA TASAR (Traffic Aware Strategic Aircrew Requests) concept are described, each providing incrementally more efficiency and capacity benefits to airspace system users and service providers, culminating in a Full Airborne Trajectory Management capability. For each of these steps, the incremental Operational Hazards and Safety Requirements are identified for later use in future formal safety assessments intended to lead to certification and operational approval of the equipment and the associated procedures. Two established safety assessment methodologies that are compliant with the FAA's Safety Management System were used leading to Failure Effects Classifications (FEC) for each of the steps. The most likely FEC for the first three steps, Basic TASAR, Digital TASAR, and 4D TASAR, is "No effect". For step four, Strategic Airborne Trajectory Management, the likely FEC is "Minor". For Full Airborne Trajectory Management (Step 5), the most likely FEC is "Major".

  20. Robust identification of noncoding RNA from transcriptomes requires phylogenetically-informed sampling.

    Directory of Open Access Journals (Sweden)

    Stinus Lindgreen

    2014-10-01

    Full Text Available Noncoding RNAs are integral to a wide range of biological processes, including translation, gene regulation, host-pathogen interactions and environmental sensing. While genomics is now a mature field, our capacity to identify noncoding RNA elements in bacterial and archaeal genomes is hampered by the difficulty of de novo identification. The emergence of new technologies for characterizing transcriptome outputs, notably RNA-seq, are improving noncoding RNA identification and expression quantification. However, a major challenge is to robustly distinguish functional outputs from transcriptional noise. To establish whether annotation of existing transcriptome data has effectively captured all functional outputs, we analysed over 400 publicly available RNA-seq datasets spanning 37 different Archaea and Bacteria. Using comparative tools, we identify close to a thousand highly-expressed candidate noncoding RNAs. However, our analyses reveal that capacity to identify noncoding RNA outputs is strongly dependent on phylogenetic sampling. Surprisingly, and in stark contrast to protein-coding genes, the phylogenetic window for effective use of comparative methods is perversely narrow: aggregating public datasets only produced one phylogenetic cluster where these tools could be used to robustly separate unannotated noncoding RNAs from a null hypothesis of transcriptional noise. Our results show that for the full potential of transcriptomics data to be realized, a change in experimental design is paramount: effective transcriptomics requires phylogeny-aware sampling.

  1. R&D Requirements, RF Gun Mode Studies, FEL-2 Steady-StateStudies, Preliminary FEL-1 Time-Dependent Studies, and Preliminary LayoutOption Investigation

    Energy Technology Data Exchange (ETDEWEB)

    Byrd, John; Corlett, John; Doolittle, Larry; Fawley, William; Lidia, Steven; Penn, Gregory; Ratti, Alex; Staples, John; Wilcox Russell; Wurtele, Jonathan; Zholents, Alexander

    2005-10-01

    This report constitutes the third deliverable of LBNLs contracted role in the FERMI {at} Elettra Technical Optimization study. It describes proposed R&D activities for the baseline design of the Technical Optimization Study, initial studies of the RF gun mode-coupling and potential effects on beam dynamics, steady-state studies of FEL-2 performance to 10 nm, preliminary studies of time-dependent FEL-1 performance using electron bunch distribution from the start-to-end studies, and a preliminary investigation of a configuration with FEL sinclined at a small angle from the line of the linac.

  2. Development of potentiometric equipment for the identification of altered dry-cured hams: A preliminary study.

    Science.gov (United States)

    Girón, Joel; Gil-Sánchez, Luís; García-Breijo, Eduardo; Pagán, M Jesús; Barat, José M; Grau, Raúl

    2015-08-01

    Microbiological contamination in dry-cured ham can occur in the early stages of the process, a large number of microorganisms involved in spoilage can produce alterations in the product. These include non-common odours, which are detected at the end of the process by a procedure called "cala", consisting of a sharp instrument punctured in every ham; this is smelled by an expert taster, who classifies hams as good and altered hams. An electronic device would be suitable for this process given the large amount of hams. The present research aims to develop objective equipment based on the potentiometry technique that identifies altered hams. A probe was developed, containing silver, nickel and copper electrodes, and was employed to classify altered and unaltered hams prior to classification by a tester. The results shown lower Ag and higher Cu potential values for altered hams. The differences in potentiometric response reveal a classification model, although further studies are required to obtain a reliable classification model.

  3. Identification of feigned mental retardation using the new generation of malingering detection instruments: preliminary findings.

    Science.gov (United States)

    Graue, Lili O; Berry, David T R; Clark, Jessica A; Sollman, Myriam J; Cardi, Michelle; Hopkins, Jaclyn; Werline, Dellynda

    2007-12-01

    A recent Supreme Court decision--Atkins v. Virginia, 536 U.S. 304 (2002)--prohibiting the execution of mentally retarded (MR) defendants may have raised the attractiveness of feigning this condition in the criminal justice system. Unfortunately, very few published studies have addressed the detection of feigned MR. The present report compared results from tests of intelligence, psychiatric feigning, and neurocognitive faking in a group of 26 mild MR participants (MR) and 25 demographically matched community volunteers asked to feign MR (CVM). Results showed that the CVM suppressed their IQ scores to approximate closely the level of MR participants. WAIS-III and psychiatric malingering measures were relatively ineffective at discriminating feigned from genuine MR. Although neurocognitive malingering tests were more accurate, their reduced specificity in MR participants was of potential concern. Revised cutting scores, set to maintain a Specificity rate of about .95 in MR clients, were identified, although they require cross-validation. Overall, these results suggest that new cutting scores will likely need to be validated to detect feigned MR using current malingering instruments.

  4. High-level waste storage tank farms/242-A evaporator Standards/Requirements Identification Document (S/RID)

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The High-Level Waste Storage Tank Farms/242-A Evaporator Standards/Requirements Identification Document (S/RID) is contained in multiple volumes. This document (Volume 3) presents the standards and requirements for the following sections: Safeguards and Security, Engineering Design, and Maintenance.

  5. 32 CFR 643.22 - Policy-Public safety: Requirement for early identification of lands containing dangerous materials.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Policy-Public safety: Requirement for early... Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE Policy § 643.22 Policy—Public safety: Requirement for early identification of lands containing dangerous materials. (a) DA...

  6. High-level waste storage tank farms/242-A evaporator Standards/Requirements Identification Document (S/RID), Volume 5

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The High-Level Waste Storage Tank Farms/242-A Evaporator Standards/Requirements Identification Document (S/RID) is contained in multiple volumes. This document (Volume 5) outlines the standards and requirements for the Fire Protection and Packaging and Transportation sections.

  7. High level waste storage tank farms/242-A evaporator Standards/Requirements Identification Document (S/RID), Volume 6

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The High-Level Waste Storage Tank Farms/242-A Evaporator Standards/Requirements Identification Document (S/RID) is contained in multiple volumes. This document (Volume 6) outlines the standards and requirements for the sections on: Environmental Restoration and Waste Management, Research and Development and Experimental Activities, and Nuclear Safety.

  8. High-level waste storage tank farms/242-A evaporator Standards/Requirements Identification Document (S/RID), Volume 4

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The High-Level Waste Storage Tank Farms/242-A Evaporator Standards/Requirements Identification Document (S/RID) is contained in multiple volumes. This document (Volume 4) presents the standards and requirements for the following sections: Radiation Protection and Operations.

  9. Preliminary assessment of numerical data requirements TA-73 landfill Los Alamos, New Mexico

    Energy Technology Data Exchange (ETDEWEB)

    1993-11-19

    A numerical model, TOUGH2, was selected for describing liquid- and gas-phase flow in the unsaturated tuff underlying the TA-73 landfill. The model was selected primarily for its ability to simulate the significant mechanisms that may affect transport of contaminants through the vadose zone at the TA-73 landfill, including non-isothermal flow through fractured media. TOUGH2 is the best documented, verified, and validated model capable of performing the required simulations. The sensitivity analyses that were performed and describes in this report identified the input parameters that the selected numerical model is most sensitive to. The input parameters analyzed were saturated hydraulic conductivity, van Genuchten {alpha} and n, residual and saturated moisture contents, infiltration rate, fracture spacing and permeability, atmospheric pressure, and temperature. The sensitivity analyses were performed using a model grid that was designed to incorporate the regions in the landfill vicinity where contaminant transport is likely to occur and where the physical processes affecting flow and transport are the most dynamic. The sensitivity analyses performed suggest that the model is quite sensitive to a number of input parameters, including saturated hydraulic conductivity, the van Genuchten parameters {alpha} and n (for both the tuff matrix and fractures), fracture density and aperture, and atmospheric pressure. The results indicate that additional site-specific hydraulic properties and fracture data should be obtained before attempting to perform predictive, numerical simulations of gas- and liquid-phase flow beneath the landfill.

  10. Building a transnational biosurveillance network using semantic web technologies: requirements, design, and preliminary evaluation.

    Science.gov (United States)

    Teodoro, Douglas; Pasche, Emilie; Gobeill, Julien; Emonet, Stéphane; Ruch, Patrick; Lovis, Christian

    2012-05-29

    Antimicrobial resistance has reached globally alarming levels and is becoming a major public health threat. Lack of efficacious antimicrobial resistance surveillance systems was identified as one of the causes of increasing resistance, due to the lag time between new resistances and alerts to care providers. Several initiatives to track drug resistance evolution have been developed. However, no effective real-time and source-independent antimicrobial resistance monitoring system is available publicly. To design and implement an architecture that can provide real-time and source-independent antimicrobial resistance monitoring to support transnational resistance surveillance. In particular, we investigated the use of a Semantic Web-based model to foster integration and interoperability of interinstitutional and cross-border microbiology laboratory databases. Following the agile software development methodology, we derived the main requirements needed for effective antimicrobial resistance monitoring, from which we proposed a decentralized monitoring architecture based on the Semantic Web stack. The architecture uses an ontology-driven approach to promote the integration of a network of sentinel hospitals or laboratories. Local databases are wrapped into semantic data repositories that automatically expose local computing-formalized laboratory information in the Web. A central source mediator, based on local reasoning, coordinates the access to the semantic end points. On the user side, a user-friendly Web interface provides access and graphical visualization to the integrated views. We designed and implemented the online Antimicrobial Resistance Trend Monitoring System (ARTEMIS) in a pilot network of seven European health care institutions sharing 70+ million triples of information about drug resistance and consumption. Evaluation of the computing performance of the mediator demonstrated that, on average, query response time was a few seconds (mean 4.3, SD 0.1 × 10

  11. 12 CFR 792.55 - Times, places, and requirements for identification of individuals making requests and...

    Science.gov (United States)

    2010-01-01

    ... identity by a signature, address, date of birth, employee identification number if any, and one other... of a birth certificate showing parentage or a court order establishing guardianship. (c) A record may... identification of individuals making requests and identification of records requested. 792.55 Section...

  12. High level waste storage tank farms/242-A evaporator standards/requirements identification document phase 1 assessment report

    Energy Technology Data Exchange (ETDEWEB)

    Biebesheimer, E., Westinghouse Hanford Co.

    1996-09-30

    This document, the Standards/Requirements Identification Document (S/RID) Phase I Assessment Report for the subject facility, represents the results of an Administrative Assessment to determine whether S/RID requirements are fully addressed by existing policies, plans or procedures. It contains; compliance status, remedial actions, and an implementing manuals report linking S/RID elements to requirement source to implementing manual and section.

  13. Preliminary identification of potentially disruptive scenarios at the Greater Confinement Disposal Facility, Area 5 of the Nevada Test Site

    Energy Technology Data Exchange (ETDEWEB)

    Guzowski, R.V. [Science Applications International Corp., Albuquerque, NM (United States); Newman, G. [GRAM, Inc., Albuquerque, NM (United States)

    1993-12-01

    The Greater Confinement Disposal location is being evaluated to determine whether defense-generated transuranic waste buried at this location complies with the Containment Requirements established by the US Environmental Protection Agency. One step in determining compliance is to identify those combinations of events and processes (scenarios) that define possible future states of the disposal system for which performance assessments must be performed. An established scenario-development procedure was used to identify a comprehensive set of mutually exclusive scenarios. To assure completeness, 761 features, events, processes, and other listings (FEPS) were compiled from 11 references. This number was reduced to 205 primarily through the elimination of duplications. The 205 FEPs were screened based on site-specific, goal-specific, and regulatory criteria. Four events survived screening and were used in preliminary scenario development: (1) exploratory drilling penetrates a GCD borehole, (2) drilling of a withdrawal/injection well penetrates a GCD borehole, (3) subsidence occurs at the RWMS, and (4) irrigation occurs at the RWMS. A logic diagram was used to develop 16 scenarios from the four events. No screening of these scenarios was attempted at this time. Additional screening of the currently retained events and processes will be based on additional data and information from site-characterization activities. When screening of the events and processes is completed, a final set of scenarios will be developed and screened based on consequence and probability of occurrence.

  14. 31 CFR 256.13 - Are agencies required to supply a taxpayer identification number (TIN) when submitting a request...

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Are agencies required to supply a taxpayer identification number (TIN) when submitting a request for payment? 256.13 Section 256.13 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE...

  15. A survey of applications and requirements of unique identification systems and RFID techniques

    NARCIS (Netherlands)

    Ilie-Zudor, Elisabeth; Kemeny, Zsolt; van Blommestein, Fred; Monostori, Laszlo; van der Meulen, Andre

    2011-01-01

    The paper contains an overview of unique identification issues and of the various radio frequency identification techniques that are available now or will become available in the short term. The paper also compares REID with traditional ID technologies. It shows application possibilities and gives e

  16. High-level waste storage tank farms/242-A evaporator standards/requirements identification document (S/RID), Vol. 7

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    This Requirements Identification Document (RID) describes an Occupational Health and Safety Program as defined through the Relevant DOE Orders, regulations, industry codes/standards, industry guidance documents and, as appropriate, good industry practice. The definition of an Occupational Health and Safety Program as specified by this document is intended to address Defense Nuclear Facilities Safety Board Recommendations 90-2 and 91-1, which call for the strengthening of DOE complex activities through the identification and application of relevant standards which supplement or exceed requirements mandated by DOE Orders. This RID applies to the activities, personnel, structures, systems, components, and programs involved in maintaining the facility and executing the mission of the High-Level Waste Storage Tank Farms.

  17. High-level waste storage tank farms/242-A evaporator standards/requirements identification document (S/RID), Vol. 1

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The purpose of this Requirements Identification Document (RID) section is to identify, in one location, all of the facility specific requirements and good industry practices which are necessary or important to establish an effective Issues Management Program for the Tank Farm Facility. The Management Systems Functional Area includes the site management commitment to environmental safety and health (ES&H) policies and controls, to compliance management, to development and management of policy and procedures, to occurrence reporting and corrective actions, resource and issue management, and to the self-assessment process.

  18. Session 2 – Identification of endocrine disruptors and amendments of standard information requirements

    DEFF Research Database (Denmark)

    Christiansen, Sofie; Holbech, Henrik

    2014-01-01

    Discussions regarding regulation of endocrine disruptors (EDs) and combination effects are ongoing in Europe. Among the central topics of discussion are establishment of criteria for identification of EDs, whether there is a threshold for endocrine disrupting effects and how EDs should be handled...

  19. 6 CFR 37.17 - Requirements for the surface of the driver's license or identification card.

    Science.gov (United States)

    2010-01-01

    ... identification card. To be accepted by a Federal agency for official purposes, REAL ID driver's licenses and... the individual is protected from disclosure of information pursuant to section 384 of the Illegal... Organization (ICAO) 9303, “Machine Readable Travel Documents,” Volume 1, Part 1, Sixth Edition, 2006....

  20. 47 CFR 73.1212 - Sponsorship identification; list retention; related requirements.

    Science.gov (United States)

    2010-10-01

    ... consideration for an identification of any person, product, service, trademark, or brand name beyond an..., association or other unincorporated group, or other entity by whom or on whose behalf such payment is made or... of public importance and a corporation, committee, association or other unincorporated group, or...

  1. 78 FR 27335 - Transportation Worker Identification Credential (TWIC)-Reader Requirements

    Science.gov (United States)

    2013-05-10

    ... Credential (TWIC)--Reader Requirements AGENCY: Coast Guard, DHS. ACTION: Proposed rule; extension of comment...)--Reader Requirements'' for 30 days. This extension of the comment period is designed to accommodate... Credential (TWIC)--Reader Requirements'' on March 22, 2013 (78 FR 17782) that proposes to require owners and...

  2. Systematic Identification of Genes Required for Expression of Androgen Receptor Splice Variants

    Science.gov (United States)

    2016-08-01

    antibody Figure 1: Identification of hnRNP A1 as a PCGEM1 binding partner. A. A representative silver-stained gel picture showing a ~37 kDa band... Fluor 568 (Life Technology). Immunofluoresence staining Immunofluoresence staining was used to detect AR, hnRNP A1 and U2AF65, as described...secondary antibody conjugated with either Alexa Fluor 568 or Alexa Fluor 488 in the dark for 1 h. For nuclear staining, the cells were subsequently

  3. Preliminary Data on the Ecological Requirements of the Invasive Spiny-Cheek Crayfish in the Lower Danube

    Directory of Open Access Journals (Sweden)

    Pîrvu Mălina

    2014-12-01

    Full Text Available Water quality and properties of the riverbed often shape the community structure of aquatic ecosystems, occasionally sustaining the expansion of non-native species. This study aims to provide preliminary data on the ecological preferences of the invasive species Orconectes limosus, its control, and the protection of the native stock is an European priority. In order to assess the species ability to colonize small river systems, relevant tributaries in the invaded Danube sector were monitored. Statistical test indicates a preference for deep and warm rivers, low water velocity and also high concentrations of calcium.

  4. Superfluid helium orbital resupply - The status of the SHOOT flight experiment and preliminary user requirements. [Superfluid Helium On-Orbit Transfer

    Science.gov (United States)

    Dipirro, Michael J.; Kittel, Peter

    1989-01-01

    The Superfluid Helium On-Orbit Transfer (SHOOT) flight experiment is designed to demonstrate the components and techniques necessary to resupply superfluid helium to satellites or Space Station based facilities. A top level description as well as the development status of the critical components to be used in SHOOT are discussed. Some of these components include the thermomechanical pump, the fluid acquisition system, the normal helium and superfluid helium phase separators, Venturi flow meter, cryogenic valves, burst disks, and astronaut-compatible EVA coupler and transfer line. The requirements for the control electronics and software are given. A preliminary description of the requirements that must be met by a satellite requiring superfluid helium servicing is given. In particular, minimum and optimum plumbing arrangements are shown, transfer line flow impedance and heat input impacts are assessed, instrumentation is described, and performance parameters are considered.

  5. Preliminary research on the identification system for anthracnose and powdery mildew of sandalwood leaf based on image processing.

    Science.gov (United States)

    Wu, Chunyan; Wang, Xuefeng

    2017-01-01

    This paper presents a survey on a system that uses digital image processing techniques to identify anthracnose and powdery mildew diseases of sandalwood from digital images. Our main objective is researching the most suitable identification technology for the anthracnose and powdery mildew diseases of the sandalwood leaf, which provides algorithmic support for the real-time machine judgment of the health status and disease level of sandalwood. We conducted real-time monitoring of Hainan sandalwood leaves with varying severity levels of anthracnose and powdery mildew beginning in March 2014. We used image segmentation, feature extraction and digital image classification and recognition technology to carry out a comparative experimental study for the image analysis of powdery mildew, anthracnose disease and healthy leaves in the field. Performing the actual test for a large number of diseased leaves pointed to three conclusions: (1) Distinguishing effects of BP (Back Propagation) neural network method, in all kinds of classical methods, for sandalwood leaf anthracnose and powdery mildew disease are relatively good; the size of the lesion areas were closest to the actual. (2) The differences between two diseases can be shown well by the shape feature, color feature and texture feature of the disease image. (3) Identifying and diagnosing the diseased leaves have ideal results by SVM, which is based on radial basis kernel function. The identification rate of the anthracnose and healthy leaves was 92% respectively, and that of powdery mildew was 84%. Disease identification technology lays the foundation for remote monitoring disease diagnosis, preparing for remote transmission of the disease images, which is a very good guide and reference for further research of the disease identification and diagnosis system in sandalwood and other species of trees.

  6. High-throughput identification of purification conditions leads to preliminary crystallization conditions for three inner membrane proteins.

    Science.gov (United States)

    Gabrielsen, Mads; Kroner, Frank; Black, Isobel; Isaacs, Neil W; Roe, Andrew J; McLuskey, Karen

    2011-01-01

    An important factor in the crystallization, and subsequent structural determination, of integral membrane proteins is the ability to produce a stable and monodisperse solution of the protein. Obtaining the correct purification detergent to achieve this can be laborious and is often serendipitous. In this study, high-throughput methods are used to analyze the suitability of eight different detergents on the stability of 12 inner transmembrane proteins from Escherichia coli. The best results obtained from the small-scale experiments were scaled up, the aggregation state of the proteins assessed, and all monodisperse protein solutions entered into crystallization trials. This resulted in preliminary crystallization hits for three inner membrane proteins: XylH, PgpB and YjdL and this study reports the methods, purification procedures and crystallization conditions used to achieve this.

  7. Identification of quantitative trait locus (QTL) linked to dorsal fin length from preliminary linkage map of molly fish, Poecilia sp.

    Science.gov (United States)

    Keong, Bun Poh; Siraj, Siti Shapor; Daud, Siti Khalijah; Panandam, Jothi Malar; Rahman, Arina Nadia Abdul

    2014-02-15

    A preliminary linkage map was constructed by applying backcross and testcross strategy using microsatellite (SSR) markers developed for Xiphophorus and Poecilia reticulata in ornamental fish, molly Poecilia sp. The linkage map having 18 SSR loci consisted of four linkage groups that spanned a map size of 516.1cM. Association between genotypes and phenotypes was tested in a random fashion and QTL for dorsal fin length was found to be linked to locus Msb069 on linkage group 2. Coincidentally, locus Msb069 was also reported as putative homologue primer pairs containing SSRs repeat motif which encoded hSMP-1, a sex determining locus. Dorsal fin length particularly in males of Poecilia latipinna is an important feature during courtship display. Therefore, we speculate that both dorsal fin length and putative hSMP-1 gene formed a close proximity to male sexual characteristics.

  8. Neuronal target identification requires AHA-1-mediated fine-tuning of Wnt signaling in C. elegans.

    Directory of Open Access Journals (Sweden)

    Jingyan Zhang

    2013-06-01

    Full Text Available Electrical synaptic transmission through gap junctions is a vital mode of intercellular communication in the nervous system. The mechanism by which reciprocal target cells find each other during the formation of gap junctions, however, is poorly understood. Here we show that gap junctions are formed between BDU interneurons and PLM mechanoreceptors in C. elegans and the connectivity of BDU with PLM is influenced by Wnt signaling. We further identified two PAS-bHLH family transcription factors, AHA-1 and AHR-1, which function cell-autonomously within BDU and PLM to facilitate the target identification process. aha-1 and ahr-1 act genetically upstream of cam-1. CAM-1, a membrane-bound receptor tyrosine kinase, is present on both BDU and PLM cells and likely serves as a Wnt antagonist. By binding to a cis-regulatory element in the cam-1 promoter, AHA-1 enhances cam-1 transcription. Our study reveals a Wnt-dependent fine-tuning mechanism that is crucial for mutual target cell identification during the formation of gap junction connections.

  9. An empirical analysis of the required management skills in the core employees' identification

    Directory of Open Access Journals (Sweden)

    Natalia García Carbonell

    2016-01-01

    Full Text Available The current study empirically analyses the influence of top management team human capital attributes on one of the most relevant stages in the human resource management strategy formulation: the core employees' identification. Drawing on recent calls from the strategic human resource management literature, this study proposes a "process" perspective instead of the traditional "content" analysis, with the intention of going a step further on the internal dynamic of these strategic processes. Applying the structural equation modeling via Partial Least Square (PLS on a sample of 120 Spanish firms, results reveal that critical human resources identification processes demand mixed cognitive skills, rational and creative ones, in order to complete efficiently different steps of the process. Consequently, to reach a balanced combination of previous skills, collectivistic dynamics are needed, fostering cooperative and collaborative decision making processes. In this context, HR managers will participate improving the process with his/her expert power and developing technical HR activities; subsequently, the HR information will be integrated the strategic decision making process with the rest of the team. In addition, interesting professional implications arise from the study in relation to the presence of the cognitive diversity in top management teams.

  10. Preliminary phenomena identification and ranking tables for simplified boiling water reactor Loss-of-Coolant Accident scenarios

    Energy Technology Data Exchange (ETDEWEB)

    Kroeger, P.G.; Rohatgi, U.S.; Jo, J.H.; Slovik, G.C.

    1998-04-01

    For three potential Loss-of-Coolant Accident (LOCA) scenarios in the General Electric Simplified Boiling Water Reactors (SBWR) a set of Phenomena Identification and Ranking Tables (PIRT) is presented. The selected LOCA scenarios are typical for the class of small and large breaks generally considered in Safety Analysis Reports. The method used to develop the PIRTs is described. Following is a discussion of the transient scenarios, the PIRTs are presented and discussed in detailed and in summarized form. A procedure for future validation of the PIRTs, to enhance their value, is outlined. 26 refs., 25 figs., 44 tabs.

  11. BREATH-HOLD CINE-MRI DURING DIPYRIDAMOLE STRESS FOR IDENTIFICATION OF SEVERE CORONARY ARTERY STENOSIS:A PRELIMINARY REPORT

    Institute of Scientific and Technical Information of China (English)

    赵世华; DidierRevel; PierreCroisille; JeanP.Roux; MarcJanier; IsabelleMagnin

    1998-01-01

    Breath-hold cine MRI was employed for the identification of severe coronary artery stenoscs after 0.56mg/kg of dipyridamole was infused. Fourteen patients without myocardial infarction but with ≥70% diameter narrowing of 1. or 2 major coronary artaries were studied. Each patient tmderwent coronary angiography, MRI at rest and during stress. Segmental wall motion abnormalities were visually assessed in a cine loop, followed by quantitative analysis by calculation of percent systolic wall thickening(%SWth). The results showed that the sensitivities of 70% and 90% were present for the qualitative and quantitative analyses, respectively, in respect to the approximate specificities(82% & 86%). Further quantitative analysis showed that sensitivities and specifieities were 88% and 88% for 1-vessel disease versus 92% and 83% for 2-vessd disease; 86% and 100% for LAD, 100% and 70% for LCX, 89% and 100% for RCA. We concloded that quantitative analysis is significantly superior to qualitative analysis for the identification of severe coronary stenosis while dipyridamole-induced wall motion abnormalities were assessed by breath-hold cine MRI.

  12. Application of factorial design to accelerate identification of CHO growth factor requirements.

    Science.gov (United States)

    Chun, Chung; Heineken, Katy; Szeto, Dongmei; Ryll, Thomas; Chamow, Steve; Chung, John D

    2003-01-01

    To accelerate recombinant CHO media and process development, we describe a simple approach to integrating multiple tasks associated with these processes including initial media design, serum-free adaptation, stability analysis and first generation scale-up. Factorial design techniques and normal probability chart representation of the results were first applied to identify potent parental CHO cell growth factors in a lean basal medium. These results were then applied to identify a suitable manufacturing medium from a panel of commercial and proprietary media formulations. When this approach was applied to recombinant CHO cell line, rapid adaptation of the cell line to an appropriate production medium occurred during culture expansion in the presence of the identified growth factor(s). This approach allows media component screening to be naturally integrated into the adaptation and scale-up processes since components that have little or no relative effect on cell proliferation are selected against as the "best" cultures are moved forward. The rapidity of the adaptation process allowed cell line stability studies to be initiated relatively early in the development process, thus providing preliminary stability information by the time the "outgrowing" culture could be scaled to 100-L reactors some 30 days after adaptation commenced. The application of full factorial design techniques allowed us to calculate the maximum number of interaction effects, the interpretation of which we believe can provide insights into growth factor biology.

  13. High-level waste storage tank farms/242-A evaporator standards/requirements identification document (S/RID), Vol. 3

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The Safeguards and Security (S&S) Functional Area address the programmatic and technical requirements, controls, and standards which assure compliance with applicable S&S laws and regulations. Numerous S&S responsibilities are performed on behalf of the Tank Farm Facility by site level organizations. Certain other responsibilities are shared, and the remainder are the sole responsibility of the Tank Farm Facility. This Requirements Identification Document describes a complete functional Safeguards and Security Program that is presumed to be the responsibility of the Tank Farm Facility. The following list identifies the programmatic elements in the S&S Functional Area: Program Management, Protection Program Scope and Evaluation, Personnel Security, Physical Security Systems, Protection Program Operations, Material Control and Accountability, Information Security, and Key Program Interfaces.

  14. The identification of novel loci required for appropriate nodule development in Medicago truncatula

    OpenAIRE

    Domonkos, Agota; Horvath, Beatrix; Marsh, John F.; Halasz, Gabor; Ayaydin, Ferhan; Oldroyd, Giles ED; Kalo, Peter

    2013-01-01

    Background The formation of functional symbiotic nodules is the result of a coordinated developmental program between legumes and rhizobial bacteria. Genetic analyses in legumes have been used to dissect the signaling processes required for establishing the legume-rhizobial endosymbiotic association. Compared to the early events of the symbiotic interaction, less attention has been paid to plant loci required for rhizobial colonization and the functioning of the nodule. Here we describe the i...

  15. A simple tool for preliminary hazard identification and quick assessment in craftwork and small/medium enterprises (SME).

    Science.gov (United States)

    Colombini, Daniela; Occhipinti, E; Di Leone, G

    2012-01-01

    During the last Congress of the International Ergonomics Association (IEA), Beijing, August 2009, an international group was founded aimed at developing a "toolkit for MSD prevention" within IEA and in collaboration with World Health Organization (WHO). Possible users of toolkits are: members of health and safety committees, health and safety representatives, line supervisors; labor inspectors; health workers implementing basic occupational health services; occupational health and safety specialists.According to ISO standard 11228 series and the new Draft CD ISO 12259-2009: Application document guides for the potential user, a computer software ( in Excel®) was create dealing with hazard "mapping" in handicraft The proposed methodology, using specific key enters and quick assessment criteria, allows a simple ergonomics hazard identification and risk estimation. Thus it makes possible to decide for which professional hazards a more exhaustive risk assessment will be necessary and which professional consultant should be involved (occupational physician, safety engineer, industrial hygienist, etc.).

  16. IT Knowledge Requirements Identification in Organizational Networks: Cooperation Between Industrial Organizations and Universities

    Science.gov (United States)

    Rudzajs, Peteris; Kirikova, Marite

    ICT professionals face rapid technology development, changes in design paradigms, methodologies, approaches, and cooperation patterns. These changes impact relationships between universities that teach ICT disciplines and industrial organizations that develop and use ICT-based products. The required knowledge and skills of university graduates depend mainly on the current industrial situation; therefore the university graduates have to meet industry requirements which are stated at the time point of their graduation, not at the start of their studies. Continuous cooperation between universities and industrial organizations is needed to identify a time and situation-dependent set of knowledge requirements, which lead to situation aware, industry acknowledged, balanced and productive ICT study programs. This chapter proposes information systems solutions supporting cooperation between the university and the industrial organizations with respect to curriculum development in ICT area.

  17. Identification of chilling and heat requirements of cherry trees—a statistical approach

    Science.gov (United States)

    Luedeling, Eike; Kunz, Achim; Blanke, Michael M.

    2013-09-01

    Most trees from temperate climates require the accumulation of winter chill and subsequent heat during their dormant phase to resume growth and initiate flowering in the following spring. Global warming could reduce chill and hence hamper the cultivation of high-chill species such as cherries. Yet determining chilling and heat requirements requires large-scale controlled-forcing experiments, and estimates are thus often unavailable. Where long-term phenology datasets exist, partial least squares (PLS) regression can be used as an alternative, to determine climatic requirements statistically. Bloom dates of cherry cv. `Schneiders späte Knorpelkirsche' trees in Klein-Altendorf, Germany, from 24 growing seasons were correlated with 11-day running means of daily mean temperature. Based on the output of the PLS regression, five candidate chilling periods ranging in length from 17 to 102 days, and one forcing phase of 66 days were delineated. Among three common chill models used to quantify chill, the Dynamic Model showed the lowest variation in chill, indicating that it may be more accurate than the Utah and Chilling Hours Models. Based on the longest candidate chilling phase with the earliest starting date, cv. `Schneiders späte Knorpelkirsche' cherries at Bonn exhibited a chilling requirement of 68.6 ± 5.7 chill portions (or 1,375 ± 178 chilling hours or 1,410 ± 238 Utah chill units) and a heat requirement of 3,473 ± 1,236 growing degree hours. Closer investigation of the distinct chilling phases detected by PLS regression could contribute to our understanding of dormancy processes and thus help fruit and nut growers identify suitable tree cultivars for a future in which static climatic conditions can no longer be assumed. All procedures used in this study were bundled in an R package (`chillR') and are provided as Supplementary materials. The procedure was also applied to leaf emergence dates of walnut (cv. `Payne') at Davis, California.

  18. On Early Conflict Identification by Requirements Modeling of Energy System Control Structures

    DEFF Research Database (Denmark)

    Heussen, Kai; Gehrke, Oliver; Niemann, Hans Henrik

    2015-01-01

    Control systems are purposeful systems involving goal-oriented information processing (cyber) and technical (physical) structures. Requirements modeling formalizes fundamental concepts and relations of a system architecture at a high-level design stage and can be used to identify potential design...... at later design stages. However, languages employed for requirements modeling today do not offer the expressiveness necessary to represent control purposes in relation to domain level interactions and therefore miss several types of interdependencies. This paper introduces the idea of control structure...

  19. Identification of Requirements for Distribution Management Systems in the Smart Grid Context

    DEFF Research Database (Denmark)

    Rezkalla, Michel M.N.; Heussen, Kai; Marinelli, Mattia

    2015-01-01

    The integration of significant volumes of distributed and renewable energy resources directly connected to the distribution network raises new requirement to maintain and operate the power system in secure state. Thus the Distribution Management System (DMS) needs to be updated and integrated...... with new functionality to provide effective support for the operators. The DMS is a control center solution that provides the needed functionality for the management of medium and low voltage distribution networks. This paper aims to provide an overview of the main functions present in today´s DMS...... platforms and to identify the new requirements to better serve in a smart grid context....

  20. Identification of host cell factors required for intoxication through use of modified cholera toxin.

    Science.gov (United States)

    Guimaraes, Carla P; Carette, Jan E; Varadarajan, Malini; Antos, John; Popp, Maximilian W; Spooner, Eric; Brummelkamp, Thijn R; Ploegh, Hidde L

    2011-11-28

    We describe a novel labeling strategy to site-specifically attach fluorophores, biotin, and proteins to the C terminus of the A1 subunit (CTA1) of cholera toxin (CTx) in an otherwise correctly assembled and active CTx complex. Using a biotinylated N-linked glycosylation reporter peptide attached to CTA1, we provide direct evidence that ~12% of the internalized CTA1 pool reaches the ER. We also explored the sortase labeling method to attach the catalytic subunit of diphtheria toxin as a toxic warhead to CTA1, thus converting CTx into a cytolethal toxin. This new toxin conjugate enabled us to conduct a genetic screen in human cells, which identified ST3GAL5, SLC35A2, B3GALT4, UGCG, and ELF4 as genes essential for CTx intoxication. The first four encode proteins involved in the synthesis of gangliosides, which are known receptors for CTx. Identification and isolation of the ST3GAL5 and SLC35A2 mutant clonal cells uncover a previously unappreciated differential contribution of gangliosides to intoxication by CTx.

  1. Isolation and Preliminary Identification of Oil-Degrading Microorganism%高效石油降解菌的筛选及初步鉴定

    Institute of Scientific and Technical Information of China (English)

    王伟平; 李伟; 邱雁临; 张华山; 彭晓斌

    2009-01-01

    Nine strains which could degrade crude oil efficiently were isolated from oil-containing soil or water of Jianghan Oil Field and Jidong Oil Field,and were screened on the plates and flasks respectively in the selective medium with oil as sole carbon source. The results showed that three bacteria (X-1,X-2,X-3) had relatively high capacity of oil degradation and the best one was strain X-1,with degradation rate of oil as high as 64.28%.It was belong to Arthrobacter sp. by preliminary identification through configuration observation and physiological and biochemical characteristics.%经过以石油烃为唯一碳源的选择性培养基平板初筛和三角瓶发酵复筛,采用紫外分光光度法测定石油降解率,从江汉油田和冀东油田石油污染的土壤和水体中,筛选出有降解石油能力的微生物9株.其中3株细菌(X-1,X-2,X-3)降解石油能力较高,X-1菌株的石油降解率最高达64.28%.根据形态学观察和部分生理生化特征初步鉴定,该菌为节杆菌属(Arthrobacter sp.).

  2. 78 FR 17781 - Transportation Worker Identification Credential (TWIC)-Reader Requirements

    Science.gov (United States)

    2013-03-22

    ..., and critical assets and infrastructure located on or adjacent to waters subject to the jurisdiction of... would result from a universal TWIC ] reader requirement for all vessels and facilities. Therefore, in... facility of any kind located in, on, under, or adjacent to any waters subject to the jurisdiction of...

  3. 76 FR 50180 - Proposed Information Collection; Comment Request; Northwest Region Gear Identification Requirements

    Science.gov (United States)

    2011-08-12

    ... management programs depends significantly on regulatory compliance. The requirements that fishing gear be marked are essential to facilitate enforcement. The ability to link fishing gear to the vessel owner or... concerning damage, loss, and civil proceedings. The regulations specify fishing gear must be marked with...

  4. The Identification and Comparison of the Requirements Placed on Product Managers during the Recruitment Process

    Directory of Open Access Journals (Sweden)

    Wroblowská Zuzana

    2015-09-01

    Full Text Available The submitted paper focuses on personality traits and behavioural competencies of a key role bearer in product oriented marketing management, generally referred to as product management. An interdisciplinary approach was applied while looking into this subject, since both research into theoretical bases and analysis of the current state of the topic and the tendencies of its development required work in several fields of study. Based on research in the field of secondary data, the assumption was set that a product manager is an example of a knowledge worker of the 21st century and that the business practice sees him/her as such, which has an effect on the requirements a candidate for this position is confronted with in the recruitment process. An independent research project was carried out and it confirmed that product managers are considered to be knowledge workers and that independence and analytical thinking skills were among the most common requirements for product managers both in 2007 and 2014. A comparison of results from 2007 and 2014 also showed some differences. The statistical verification confirmed a shift in requirements within the interpersonal competency group. The findings were used to formulate recommendations for the recruitment strategy and realization of selection for positions in product management.

  5. 76 FR 73517 - Fisheries in the Eastern Pacific Ocean; Pelagic Fisheries; Vessel Identification Requirements

    Science.gov (United States)

    2011-11-29

    ... WCPFC Area Endorsement are required to display their IRCS on the port and starboard sides of the hull or... ``USA'' and a hyphen (i.e., ``USA- ''). Only these markings are allowed on the hull or superstructure... markings, including at least 15 longline vessels and 8 troll and bait boat vessels. Some vessels may also...

  6. 76 FR 18706 - Fisheries in the Eastern Pacific Ocean; Pelagic Fisheries; Vessel Identification Requirements

    Science.gov (United States)

    2011-04-05

    ... Endorsement would be required to display its IRCS on the port and starboard sides of the hull or... hyphen (i.e., ``USA--''). Only these markings would be allowed on the hull or superstructure, apart from.... Dated: March 31, 2011. John Oliver, Deputy Assistant Administrator for Operations, National...

  7. High-level waste storage tank farms/242-A evaporator standards/requirements identification document (S/RID), Vol. 2

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The Quality Assurance Functional Area Requirements Identification Document (RID), addresses the programmatic requirements that ensure risks and environmental impacts are minimized, ensure safety, reliability, and performance are maximized through the application of effective management systems commensurate with the risks posed by the Tank Farm Facility and its operation. This RID incorporates guidance intended to provide Tank Farms management with the necessary requirements information to develop, upgrade, or assess the effectiveness of a Quality Assurance Program in the performance of organizational and functional activities. Quality Assurance is defined as all those planned and systematic actions necessary to provide adequate confidence that a facility, structure, system, or component will perform satisfactorily and safely in service. This document will provide the specific requirements to meet DNFSB recommendations and the guidance provided in DOE Order 5700.6C, utilizing industry codes, standards, regulatory guidelines, and industry good practices that have proven to be essential elements for an effective and efficient Quality Assurance Program as the nuclear industry has matured over the last thirty years.

  8. SU-E-I-74: Image-Matching Technique of Computed Tomography Images for Personal Identification: A Preliminary Study Using Anthropomorphic Chest Phantoms

    Energy Technology Data Exchange (ETDEWEB)

    Matsunobu, Y; Shiotsuki, K [Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Fukuoka (Japan); Morishita, J [Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Fukuoka, JP (Japan)

    2015-06-15

    Purpose: Fingerprints, dental impressions, and DNA are used to identify unidentified bodies in forensic medicine. Cranial Computed tomography (CT) images and/or dental radiographs are also used for identification. Radiological identification is important, particularly in the absence of comparative fingerprints, dental impressions, and DNA samples. The development of an automated radiological identification system for unidentified bodies is desirable. We investigated the potential usefulness of bone structure for matching chest CT images. Methods: CT images of three anthropomorphic chest phantoms were obtained on different days in various settings. One of the phantoms was assumed to be an unidentified body. The bone image and the bone image with soft tissue (BST image) were extracted from the CT images. To examine the usefulness of the bone image and/or the BST image, the similarities between the two-dimensional (2D) or threedimensional (3D) images of the same and different phantoms were evaluated in terms of the normalized cross-correlation value (NCC). Results: For the 2D and 3D BST images, the NCCs obtained from the same phantom assumed to be an unidentified body (2D, 0.99; 3D, 0.93) were higher than those for the different phantoms (2D, 0.95 and 0.91; 3D, 0.89 and 0.80). The NCCs for the same phantom (2D, 0.95; 3D, 0.88) were greater compared to those of the different phantoms (2D, 0.61 and 0.25; 3D, 0.23 and 0.10) for the bone image. The difference in the NCCs between the same and different phantoms tended to be larger for the bone images than for the BST images. These findings suggest that the image-matching technique is more useful when utilizing the bone image than when utilizing the BST image to identify different people. Conclusion: This preliminary study indicated that evaluating the similarity of bone structure in 2D and 3D images is potentially useful for identifying of an unidentified body.

  9. A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.

    Directory of Open Access Journals (Sweden)

    Laurent Dacheux

    Full Text Available The prediction of viral zoonosis epidemics has become a major public health issue. A profound understanding of the viral population in key animal species acting as reservoirs represents an important step towards this goal. Bats harbor diverse viruses, some of which are of particular interest because they cause severe human diseases. However, little is known about the diversity of the global population of viruses found in bats (virome. We determined the viral diversity of five different French insectivorous bat species (nine specimens in total in close contact with humans. Sequence-independent amplification, high-throughput sequencing with Illumina technology and a dedicated bioinformatics analysis pipeline were used on pooled tissues (brain, liver and lungs. Comparisons of the sequences of contigs and unassembled reads provided a global taxonomic distribution of virus-related sequences for each sample, highlighting differences both within and between bat species. Many viral families were present in these viromes, including viruses known to infect bacteria, plants/fungi, insects or vertebrates, the most relevant being those infecting mammals (Retroviridae, Herpesviridae, Bunyaviridae, Poxviridae, Flaviviridae, Reoviridae, Bornaviridae, Picobirnaviridae. In particular, we detected several new mammalian viruses, including rotaviruses, gammaretroviruses, bornaviruses and bunyaviruses with the identification of the first bat nairovirus. These observations demonstrate that bats naturally harbor viruses from many different families, most of which infect mammals. They may therefore constitute a major reservoir of viral diversity that should be analyzed carefully, to determine the role played by bats in the spread of zoonotic viral infections.

  10. IDENTIFICATION OF DOE'S POST-CLOSURE MONITORING NEEDS AND REQUIREMENTS

    Energy Technology Data Exchange (ETDEWEB)

    M.A. Ebadian, Ph.D.

    1999-01-01

    The 2006 plan sets an ambitious agenda for the U.S. Department of Energy (DOE), Office of Environmental Management (EM) and the remediation of sites contaminated by decades of nuclear weapons production activities. The plan's primary objective is to reduce overall clean up costs by first eliminating the environmental problems that are most expensive to control and safely maintain. In the context of the 2006 Plan, closure refers to the completion of area or facility specific cleanup projects. The cleanup levels are determined by the planned future use of the site or facility. Use restrictions are still undecided for most sites but are highly probable to exclude residential or agricultural activities. Most of the land will be remediated to ''industrial use'' levels with access restrictions and some areas will be closed-off through containment. Portions of the site will be reserved for waste disposal, either as a waste repository or the in-situ immobilization of contaminated soil and groundwater, and land use will be restricted to waste disposal only. The land used for waste disposal will require monitoring and maintenance activities after closure. Most of the land used for industrial use may also require such postclosure activities. The required postclosure monitoring and maintenance activities will be imposed by regulators and stakeholders. Regulators will not approve closure plans without clearly defined monitoring methods using approved technologies. Therefore, among all other more costly and labor-intensive closure-related activities, inadequate planning for monitoring and lack of appropriate monitoring technologies can prevent closure. The purpose of this project is to determine, document, and track the current and evolving postclosure monitoring requirements at DOE-EM sites. This information will aid CMST-CP in guiding its postclosure technology development and deployment efforts.

  11. Identification of Drosophila gene products required for phagocytosis of Candida albicans.

    Directory of Open Access Journals (Sweden)

    Shannon L Stroschein-Stevenson

    2006-01-01

    Full Text Available Phagocytosis is a highly conserved aspect of innate immunity. We used Drosophila melanogaster S2 cells as a model system to study the phagocytosis of Candida albicans, the major fungal pathogen of humans, by screening an RNAi library representing 7,216 fly genes conserved among metazoans. After rescreening the initial genes identified and eliminating certain classes of housekeeping genes, we identified 184 genes required for efficient phagocytosis of C. albicans. Diverse biological processes are represented, with actin cytoskeleton regulation, vesicle transport, signaling, and transcriptional regulation being prominent. Secondary screens using Escherichia coli and latex beads revealed several genes specific for C. albicans phagocytosis. Characterization of one of those gene products, Macroglobulin complement related (Mcr, shows that it is secreted, that it binds specifically to the surface of C. albicans, and that it promotes its subsequent phagocytosis. Mcr is closely related to the four Drosophila thioester proteins (Teps, and we show that TepII is required for efficient phagocytosis of E. coli (but not C. albicans or Staphylococcus aureus and that TepIII is required for the efficient phagocytosis of S. aureus (but not C. albicans or E. coli. Thus, this family of fly proteins distinguishes different pathogens for subsequent phagocytosis.

  12. High-level waste storage tank farms/242-A evaporator Standards/Requirements Identification Document (S/RID), Volume 7. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Burt, D.L.

    1994-04-01

    The High-Level Waste Storage Tank Farms/242-A Evaporator Standards/Requirements Identification Document (S/RID) is contained in multiple volumes. This document (Volume 7) presents the standards and requirements for the following sections: Occupational Safety and Health, and Environmental Protection.

  13. Line Identifications and Preliminary Synthesis of High-resolution Infrared Spectra of CP and Herbig Ae Stars

    Science.gov (United States)

    Cowley, Charles R.; Castelli, F.; Hubrig, S.; Wolff, B.; Elkin, V.

    2012-01-01

    We report on surveys of infrared spectra of chemically peculiar and Herbig Ae stars based on CRIRES (Kaufl, et al. SPIE, 5492, 1218 2004). We discuss the magnetic CP stars Gamma Equ and HD 154708, and multiple-phase observations of the Herbig Ae star HD 101412. The Be star HR 4537 and HgMn HR 6620 were also examined. The primary emphasis of the present work is on line identifications primarily in four regions, 1065-1091, 1084-1109,1550-1587, and 2276-2313nm (with order gaps). Observations were reduced with recipes available from the ESO CRIRES data reduction pipeline. Wavelength calibration is determined from daytime ThAr arc lamp exposures. Generally speaking, this is not rich in atomic lines. The strongest features are the Paschen line P6 (1093.81nm), and He I (108.30nm). The latter shows phase variations indicative of a more complex magnetic field than that of a pure dipole. No individual molecular lines were found in these early stars, though CO emission from circumstellar material is likely present in HR 4537 and HD 101412. We used atomic line lists from Kurucz's site (kurucz.harvard.edu) and VALD (http://vald.astro.univie.ac.at/ cf. Kupka et al. 1999, A&AS, 138, 119), supplemented by Outred (J. Phys. Chem. Ref. Data 7, 1, 1978). The following spectra were identified in Gamma Equ: C I, Si I, Ca I, Mg I, II, Cr I, Fe I, Sr II, and Ce III (1584.75nm). The Ap star spectra show broad Zeeman patterns compatible with published models and field strengths. Synthetic calculations used SYNTHE and SYNTHMAG (Piskunov N. E., 1999, in Astrophys. Space Sci. Library Vol. 243, Solar polarization. Kluwer, p 515). The γ Equ model is from Heiter et al. (2002, A&A, 392, 619). and the line list from VALD.

  14. Identification of genes required for nonhost resistance to Xanthomonas oryzae pv. oryzae reveals novel signaling components.

    Directory of Open Access Journals (Sweden)

    Wen Li

    Full Text Available BACKGROUND: Nonhost resistance is a generalized, durable, broad-spectrum resistance exhibited by plant species to a wide variety of microbial pathogens. Although nonhost resistance is an attractive breeding strategy, the molecular basis of this form of resistance remains unclear for many plant-microbe pathosystems, including interactions with the bacterial pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo. METHODS AND FINDINGS: Virus-induced gene silencing (VIGS and an assay to detect the hypersensitive response (HR were used to screen for genes required for nonhost resistance to Xoo in N. benthamiana. When infiltrated with Xoo strain YN-1, N. benthamiana plants exhibited a strong necrosis within 24 h and produced a large amount of H(2O(2 in the infiltrated area. Expression of HR- and defense-related genes was induced, whereas bacterial numbers dramatically decreased during necrosis. VIGS of 45 ACE (Avr/Cf-elicited genes revealed identified seven genes required for nonhost resistance to Xoo in N. benthamiana. The seven genes encoded a calreticulin protein (ACE35, an ERF transcriptional factor (ACE43, a novel Solanaceous protein (ACE80, a hydrolase (ACE117, a peroxidase (ACE175 and two proteins with unknown function (ACE95 and ACE112. The results indicate that oxidative burst and calcium-dependent signaling pathways play an important role in nonhost resistance to Xoo. VIGS analysis further revealed that ACE35, ACE80, ACE95 and ACE175, but not the other three ACE genes, interfered with the Cf-4/Avr4-dependent HR. CONCLUSIONS/SIGNIFICANCE: N. benthamiana plants inoculated with Xoo respond by rapidly eliciting an HR and nonhost resistance. The oxidative burst and other signaling pathways are pivotal in Xoo-N. benthamiana nonhost resistance, and genes involved in this response partially overlap with those involved in Cf/Avr4-dependent HR. The seven genes required for N. benthamiana-mediated resistance to Xoo provide a basis for further dissecting

  15. Performance assessment requirements for the identification and tracking of transuranic waste intended for disposal at the Waste Isolation Pilot Plant

    Energy Technology Data Exchange (ETDEWEB)

    Snider, C.A. [Department of Energy, Carlsbad, NM (United States); Weston, W.W. [Westinghouse Electric Corp., Carlsbad, NM (United States)

    1997-11-01

    To demonstrate compliance with environmental radiation protection standards for management and disposal of transuranic (TRU) radioactive wastes, a performance assessment (PA) of the Waste Isolation Pilot Plant (WIPP) was made of waste-waste and waste-repository interactions and impacts on disposal system performance. An estimate of waste components and accumulated quantities was derived from a roll-up of the generator/storage sites` TRU waste inventories. Waste components of significance, and some of negligible effect, were fixed input parameters in the model. The results identified several waste components that require identification and tracking of quantities to ensure that repository limits are not exceeded. The rationale used to establish waste component limits based on input estimates is discussed. The distinction between repository limits and waste container limits is explained. Controls used to ensure that no limits are exceeded are identified. For waste components with no explicit repository based limits, other applicable limits are contained in the WIPP Waste Acceptance Criteria (WAC). The 10 radionuclides targeted for identification and tracking on either a waste container or a waste stream basis include Am-241, Pu-238, Pu-239, Pu-240, Pu-242, U-233, U-234, U-238, Sr-90, and Cs-137. The accumulative activities of these radionuclides are to be inventoried at the time of emplacement in the WIPP. Changes in inventory curie content as a function of radionuclide decay and ingrowth over time will be calculated and tracked. Due to the large margin of compliance demonstrated by PA with the 10,000 year release limits specified, the quality assurance objective for radioassay of the 10 radionuclides need to be no more restrictive than those already identified for addressing the requirements imposed by transportation and WIPP disposal operations in Section 9 of the TRU Waste Characterization Quality Assurance Program Plan. 6 refs.

  16. Preliminary designs for ocean thermal energy conversion (OTEC) stationkeeping subsystems (SKSS). Task I. Design requirements. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1979-06-01

    The results of Task I, Design Requirements, are presented. Environmental conditions for the Punta Tuna, Puerto Rico site are reviewed and synthesized to provide definition of current, wind and wave severity, direction, and occurrence for service, operational, and extreme sea states. SKSS performance requirements, including design life and watch circle, are followed by interface considerations particularly for the electrical transmission riser cable, and design criteria including safety and load factors. The SKSS concepts will be analyzed to evaluate performance, reliability, and cost. Performance analysis conducted included catenary anchor leg static calculations to size components, as well as drag due to environmental loads in the operational and extreme sea states for both ship and spar platforms. Dynamic analyses and trade studies to be conducted in Task II are presented. A reliability and risk assessment analysis of the three basic SKSS types - single-, multiple-, and tension-anchor-leg moors - was completed, indicating that the multiple-anchor-leg/multiple-point rotary or turret moor has the lowest risk-criticality for the ship, while that for the spar is the multiple-anchor-leg/multiple-point moor. The catenary single-anchor-leg/single-point moor has insufficient reliability for both platforms. The life cycle cost analysis methodology, including work breakdown structure, cost estimating, and cost minimization define the approach to costing to be followed throughout the study. The results of these design trades and analyses will first be applied to concept ranking required for recommendation of a SKSS concept for each platform.

  17. Online Identification of Power Required for Self-Sustainability of the Battery in Hybrid Electric Vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Malikopoulos, Andreas [ORNL

    2014-01-01

    Hybrid electric vehicles have shown great potential for enhancing fuel economy and reducing emissions. Deriving a power management control policy to distribute the power demanded by the driver optimally to the available subsystems (e.g., the internal combustion engine, motor, generator, and battery) has been a challenging control problem. One of the main aspects of the power management control algorithms is concerned with the self-sustainability of the electrical path, which must be guaranteed for the entire driving cycle. This paper considers the problem of identifying online the power required by the battery to maintain the state of charge within a range of the target value. An algorithm is presented that realizes how much power the engine needs to provide to the battery so that self-sustainability of the electrical path is maintained.

  18. Identification and Analysis of Critical Gaps in Nuclear Fuel Cycle Codes Required by the SINEMA Program

    Energy Technology Data Exchange (ETDEWEB)

    Adrian Miron; Joshua Valentine; John Christenson; Majd Hawwari; Santosh Bhatt; Mary Lou Dunzik-Gougar: Michael Lineberry

    2009-10-01

    The current state of the art in nuclear fuel cycle (NFC) modeling is an eclectic mixture of codes with various levels of applicability, flexibility, and availability. In support of the advanced fuel cycle systems analyses, especially those by the Advanced Fuel Cycle Initiative (AFCI), Unviery of Cincinnati in collaboration with Idaho State University carried out a detailed review of the existing codes describing various aspects of the nuclear fuel cycle and identified the research and development needs required for a comprehensive model of the global nuclear energy infrastructure and the associated nuclear fuel cycles. Relevant information obtained on the NFC codes was compiled into a relational database that allows easy access to various codes' properties. Additionally, the research analyzed the gaps in the NFC computer codes with respect to their potential integration into programs that perform comprehensive NFC analysis.

  19. Requirements and criteria for the identification of research universities status in Ukraine

    Directory of Open Access Journals (Sweden)

    S.A. Tulchinskaya

    2015-03-01

    Full Text Available The aim of the article. The aim of the article is to research the existing requirements of obtaining and confirming research university status and to suggest their specifying criteria. The results of the analysis. Given the need for the higher education strategy constant development and taking into account its substantial role in the innovative development where the intellectual resources manifested in knowledge, culture, and spirituality become a crucial factor of the social and economic process, it’s important to consider the field of higher education, which has traditionally educated national elite and is concluding for the various industries training. Reviewing the education system development it is necessary to pay attention to such form of higher education as a research university, the core function of which is scientific and research activity taking different forms in different knowledge areas within universities. Currently, the fourteen Ukrainian universities have the research university status. However, a promising integration form of education, science and industry faces several local barriers like university funding; a lecturers load structure and their material incentive; an information and communication technologies development, which substantially hinders the research university model implementation. It is an established fact that the main specific features of the research university are the following: 1 big numbers of students, receiving master's and PhD degrees; 2 deep involvement of the university departments and divisions to the research activity; 3 a high training level of graduates; 4 interconnection of education and research at all levels of the educational process; 5 an established relationships with external contractors, regional and local authorities, business; 6 university international research activity, its integration with the global scientific community; 7 a high level of «academic influence» of the university at

  20. Identification and characterization of genes required for compensatory growth in Drosophila.

    Science.gov (United States)

    Gerhold, Abigail R; Richter, Daniel J; Yu, Albert S; Hariharan, Iswar K

    2011-12-01

    To maintain tissue homeostasis, some organs are able to replace dying cells with additional proliferation of surviving cells. Such proliferation can be localized (e.g., a regeneration blastema) or diffuse (compensatory growth). The relationship between such growth and the growth that occurs during development has not been characterized in detail. Drosophila melanogaster larval imaginal discs can recover from extensive damage, producing normally sized adult organs. Here we describe a system using genetic mosaics to screen for recessive mutations that impair compensatory growth. By generating clones of cells that carry a temperature-sensitive cell-lethal mutation, we conditionally ablate patches of tissue in the imaginal disc and assess the ability of the surviving sister clones to replace the lost tissue. We have used this system together with a modified whole-genome resequencing (WGS) strategy to identify several mutations that selectively compromise compensatory growth. We find specific alleles of bunched (bun) and Ribonucleoside diphosphate reductase large subunit (RnrL) reduce compensatory growth in the imaginal disc. Other genes identified in the screen, including two alleles of Topoisomerase 3-alpha (Top3α), while also required for developmental growth, appear to have an enhanced requirement during compensatory growth. Compensatory growth occurs at a higher rate than normal growth and may therefore have features in common with some types of overgrowth. Indeed, the RnrL allele identified compromises both these types of altered growth and mammalian ribonucleotide reductase and topoisomerases are targets of anticancer drugs. Finally, the approach we describe is applicable to the study of compensatory growth in diverse tissues in Drosophila.

  1. Identification of HIV-1 Vif regions required for CBF-β interaction and APOBEC3 suppression.

    Science.gov (United States)

    Wang, Hong; Liu, Bin; Liu, Xin; Li, Zhaolong; Yu, Xiao-Fang; Zhang, Wenyan

    2014-01-01

    Human immunodeficiency virus type 1 (HIV-1) Vif requires core binding factor β (CBF-β) to degrade the host APOBEC3 restriction factors. Although a minimum domain and certain amino acids of HIV-1 Vif, including hydrophobic residues at the N-terminal, have been identified as critical sites for binding with CBF-β, other regions that potentially mediate this interaction need to be further investigated. Here, we mapped two new regions of HIV-1 Vif that are required for interaction with CBF-β by generating a series of single-site or multiple-site Vif mutants and testing their effect on the suppression of APOBEC3G (A3G) and APOBEC3F (A3F). A number of the mutants, including G84A/SIEW86-89AAAA (84/86-89), E88A/W89A (88/89), G84A, W89A, L106S and I107S in the 84GxSIEW89 and L102ADQLI107 regions, affected Vif function by disrupting CBF-β binding. These Vif mutants also had altered interactions with CUL5, since CBF-β is known to facilitate the binding of Vif to CUL5. We further showed that this effect was not due to misfolding or conformational changes in Vif, as the mutants still maintained their interactions with other factors such as ElonginB, A3G and A3F. Notably, G84D and D104A had stronger effects on the Vif-CUL5 interaction than on the Vif-CBF-β interaction, indicating that they mainly influenced the CUL5 interaction and implying that the interaction of Vif with CUL5 contributes to the binding of Vif to CBF-β. These new binding interfaces with CBF-β in HIV-1 Vif provide novel targets for the development of HIV-1 inhibitors.

  2. Identification of regions required for apical membrane localization of human multidrug resistance protein 2.

    Science.gov (United States)

    Bandler, Paul E; Westlake, Christopher J; Grant, Caroline E; Cole, Susan P C; Deeley, Roger G

    2008-07-01

    Multidrug resistance proteins MRP1 and MRP2 transport a wide range of endo- and xenobiotics. However, with the exception of certain parts of the brain, MRP1 traffics to basolateral membranes of polarized cells, whereas MRP2 is apical in location and thus it is particularly important for systemic elimination of such compounds. Different regions of MRP1 and MRP2 seem to target them to their respective membrane locations. In addition to two "core" membrane spanning domains (MSDs) characteristic of ATP-binding cassette transporters, MRP1 and MRP2 have a third NH2-terminal MSD (MSD0), which is not required for basolateral targeting of MRP1, or for transport of at least some substrates. Here, we demonstrate that all elements necessary for apical targeting of MRP2 reside in MSD0 and the adjacent cytoplasmic loop (CL) 3. Furthermore, we show that this region of MRP2 can target the core of MRP1 to an exclusively apical location. Within MRP2 CL3, we identified a lysine-rich element that is essential for apical targeting. When introduced into MRP1, this element alone is sufficient to result in partial apical localization. However, exclusive targeting to the apical membrane seems to require the integrity of the entire region encompassing MSD0 and CL3 of MRP2. Because CL3 of MRP1 is critical for binding, transport, or both of several compounds, we also examined the function of hybrids containing all, or portions of MRP2 MSD0 and CL3. Our results indicate that CL3 is important for interaction with both the glutathione and glucuronide conjugates tested, but that different regions may be involved.

  3. Identification of two tyrosine residues required for the intramolecular mechanism implicated in GIT1 activation.

    Directory of Open Access Journals (Sweden)

    Antonio Totaro

    Full Text Available GIT1 is an ArfGAP and scaffolding protein regulating cell adhesion and migration. The multidomain structure of GIT1 allows the interaction with several partners. Binding of GIT1 to some of its partners requires activation of the GIT1 polypeptide. Our previous studies indicated that binding of paxillin to GIT1 is enhanced by release of an intramolecular interaction between the amino-terminal and carboxy-terminal portions that keeps the protein in a binding-incompetent state. Here we have addressed the mechanism mediating this intramolecular inhibitory mechanism by testing the effects of the mutation of several formerly identified GIT1 phosphorylation sites on the binding to paxillin. We have identified two tyrosines at positions 246 and 293 of the human GIT1 polypeptide that are needed to keep the protein in the inactive conformation. Interestingly, mutation of these residues to phenylalanine did not affect binding to paxillin, while mutation to either alanine or glutamic acid enhanced binding to paxillin, without affecting the constitutive binding to the Rac/Cdc42 exchange factor βPIX. The involvement of the two tyrosine residues in the intramolecular interaction was supported by reconstitution experiments showing that these residues are important for the binding between the amino-terminal fragment and carboxy-terminal portions of GIT1. Either GIT1 or GIT1-N tyrosine phosphorylation by Src and pervanadate treatment to inhibit protein tyrosine phosphatases did not affect the intramolecular binding between the amino- and carboxy-terminal fragments, nor the binding of GIT1 to paxillin. Mutations increasing the binding of GIT1 to paxillin positively affected cell motility, measured both by transwell migration and wound healing assays. Altogether these results show that tyrosines 246 and 293 of GIT1 are required for the intramolecular inhibitory mechanism that prevents the binding of GIT1 to paxillin. The data also suggest that tyrosine

  4. Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.

    Directory of Open Access Journals (Sweden)

    Roy R Chaudhuri

    2009-07-01

    Full Text Available Genes required for infection of mice by Salmonella Typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. Inactivation of such genes produces attenuated S. Typhimurium strains that have potential for use as live attenuated vaccines. A quantitative screen, Transposon Mediated Differential Hybridisation (TMDH, has been developed that identifies those members of a large library of transposon mutants that are attenuated. TMDH employs custom transposons with outward-facing T7 and SP6 promoters. Fluorescently-labelled transcripts from the promoters are hybridised to whole-genome tiling microarrays, to allow the position of the transposon insertions to be determined. Comparison of microarray data from the mutant library grown in vitro (input with equivalent data produced after passage of the library through mice (output enables an attenuation score to be determined for each transposon mutant. These scores are significantly correlated with bacterial counts obtained during infection of mice using mutants with individual defined deletions of the same genes. Defined deletion mutants of several novel targets identified in the TMDH screen are effective live vaccines.

  5. Identification of genes specifically required for the anaerobic metabolism of benzene in Geobacter metallireducens

    Directory of Open Access Journals (Sweden)

    Tian eZhang

    2014-05-01

    Full Text Available Although the biochemical pathways for the anaerobic degradation of many of the hydrocarbon constituents in petroleum reservoirs have been elucidated, the mechanisms for anaerobic activation of benzene, a very stable molecule, are not known. Previous studies have demonstrated that Geobacter metallireducens can anaerobically oxidize benzene to carbon dioxide with Fe(III as the sole electron acceptor and that phenol is an intermediate in benzene oxidation. In an attempt to identify enzymes that might be involved in the conversion of benzene to phenol, whole-genome gene transcript abundance was compared in cells metabolizing benzene and cells metabolizing phenol. Eleven genes had significantly higher transcript abundance in benzene-metabolizing cells. Five of these genes had annotations suggesting that they did not encode proteins that could be involved in benzene metabolism and were not further studied. Strains were constructed in which one of the remaining six genes was deleted. The strain in which the monocistronic gene Gmet 0232 was deleted metabolized phenol, but not benzene. Transcript abundance of the adjacent monocistronic gene, Gmet 0231, predicted to encode a zinc-containing oxidoreductase, was elevated in cells metabolizing benzene, although not at a statistically significant level. However, deleting Gmet 0231 also yielded a strain that could metabolize phenol, but not benzene. Although homologs of Gmet 0231 and Gmet 0232 are found in microorganisms not known to anaerobically metabolize benzene, the adjacent localization of these genes is unique to G. metallireducens. The discovery of genes that are specifically required for the metabolism of benzene, but not phenol in G. metallireducens is an important step in potentially identifying the mechanisms for anaerobic benzene activation.

  6. Identification of a mutant locus that bypasses the BsgA protease requirement for social development in Myxococcus xanthus.

    Science.gov (United States)

    Cusick, John K; Hager, Elizabeth; Gill, Ronald E

    2015-01-01

    The BsgA protease is required for the earliest morphological changes observed in Myxococcus xanthus development. We hypothesize that the BsgA protease is required to cleave an inhibitor of the developmental program, and isolation of genetic bypass suppressors of a bsgA mutant was used to identify signaling components controlling development downstream of the BsgA protease. Strain M955 was created by transposon mutagenesis of a bsgA mutant followed by screening for strains that could develop despite the absence of the BsgA protease. Strain M955 was able to aggregate, form fruiting bodies, and partially restored the production of viable spores in comparison to the parental bsgA mutant. The bsgA Tn5Ω955 strain partially restored developmental expression to a subset of genes normally induced during development, and expressed one developmentally induced fusion at higher amounts during vegetative growth in comparison to wild-type cells. The transposon in strain M955 was localized to a Ribonuclease D homolog that appears to exist in an operon with a downstream aminopeptidase-encoding gene. The identification of a third distinct bypass suppressor of the BsgA protease suggests that the BsgA protease may regulate a potentially complex pathway during the initiation of the M. xanthus developmental program.

  7. High-level waste storage tank farms/242-A evaporator standards/requirements identification document (S/RID), Vol. 4

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    Radiation protection of personnel and the public is accomplished by establishing a well defined Radiation Protection Organization to ensure that appropriate controls on radioactive materials and radiation sources are implemented and documented. This Requirements Identification Document (RID) applies to the activities, personnel, structures, systems, components, and programs involved in executing the mission of the Tank Farms. The physical boundaries within which the requirements of this RID apply are the Single Shell Tank Farms, Double Shell Tank Farms, 242-A Evaporator-Crystallizer, 242-S, T Evaporators, Liquid Effluent Retention Facility (LERF), Purgewater Storage Facility (PWSF), and all interconnecting piping, valves, instrumentation, and controls. Also included is all piping, valves, instrumentation, and controls up to and including the most remote valve under Tank Farms control at any other Hanford Facility having an interconnection with Tank Farms. The boundary of the structures, systems, components, and programs to which this RID applies, is defined by those that are dedicated to and/or under the control of the Tank Farms Operations Department and are specifically implemented at the Tank Farms.

  8. Identification of a BMP inhibitor-responsive promoter module required for expression of the early neural gene zic1.

    Science.gov (United States)

    Tropepe, Vincent; Li, Shuhong; Dickinson, Amanda; Gamse, Joshua T; Sive, Hazel L

    2006-01-15

    Expression of the transcription factor zic1 at the onset of gastrulation is one of the earliest molecular indicators of neural fate determination in Xenopus. Inhibition of bone morphogenetic protein (BMP) signaling is critical for activation of zic1 expression and fundamental for establishing neural identity in both vertebrates and invertebrates. The mechanism by which interruption of BMP signaling activates neural-specific gene expression is not understood. Here, we report identification of a 215 bp genomic module that is both necessary and sufficient to activate Xenopus zic1 transcription upon interruption of BMP signaling. Transgenic analyses demonstrate that this BMP inhibitory response module (BIRM) is required for expression in the whole embryo. Multiple consensus binding sites for specific transcription factor families within the BIRM are required for its activity and some of these regions are phylogenetically conserved between orthologous vertebrate zic1 genes. These data suggest that interruption of BMP signaling facilitates neural determination via a complex mechanism, involving multiple regulatory factors that cooperate to control zic1 expression.

  9. Identification of high-risk Dukes B colorectal cancer by microRNA expression profiling: a preliminary study.

    Science.gov (United States)

    Aslam, M I; Venkatesh, J; Jameson, J S; West, K; Pringle, J H; Singh, B

    2015-07-01

    MicroRNAs (miRNAs) from tumour tissue and common gene mutations were studied to determine whether they predict the development of metastasis in patients with Dukes B colorectal cancer. Patients who underwent curative resection for Dukes B colorectal cancer who subsequently developed distant metastatic disease at some stage in the following 5 years ('high-risk B') were compared with case-matched controls of Dukes A, Dukes B (no metastases, 'low-risk B') and Dukes C patients without any detectable metastasis at 5 years of follow-up. MiRNAs from tumour and adjacent normal tissue and common gene mutations (KRAS, BRAF, PIK3CA) in primary cancer tissue were analysed to identify prognostic tissue markers for the development of metastasis in patients with Dukes B colorectal cancer. Expression of miR-15b and miR-135b was significantly downregulated (P risk B' tumours compared with Dukes A, 'low-risk B' and C without metastasis. No significant differences were noted for mutation status and the development of metastasis. The study suggests that the development of metastasis in Dukes B tumours may be predictable based on the miRNA expression of miR-15b and miR-135b. This requires further study on a much larger cohort. Colorectal Disease © 2014 The Association of Coloproctology of Great Britain and Ireland.

  10. Targeted and random mutagenesis of Ehrlichia chaffeensis for the identification of genes required for in vivo infection.

    Science.gov (United States)

    Cheng, Chuanmin; Nair, Arathy D S; Indukuri, Vijaya V; Gong, Shanzhong; Felsheim, Roderick F; Jaworski, Deborah; Munderloh, Ulrike G; Ganta, Roman R

    2013-02-01

    Ehrlichia chaffeensis is a tick transmitted pathogen responsible for the disease human monocytic ehrlichiosis. Research to elucidate gene function in rickettsial pathogens is limited by the lack of genetic manipulation methods. Mutational analysis was performed, targeting to specific and random insertion sites within the bacterium's genome. Targeted mutagenesis at six genomic locations by homologous recombination and mobile group II intron-based methods led to the consistent identification of mutants in two genes and in one intergenic site; the mutants persisted in culture for 8 days. Three independent experiments using Himar1 transposon mutagenesis of E. chaffeensis resulted in the identification of multiple mutants; these mutants grew continuously in macrophage and tick cell lines. Nine mutations were confirmed by sequence analysis. Six insertions were located within non-coding regions and three were present in the coding regions of three transcriptionally active genes. The intragenic mutations prevented transcription of all three genes. Transposon mutants containing a pool of five different insertions were assessed for their ability to infect deer and subsequent acquisition by Amblyomma americanum ticks, the natural reservoir and vector, respectively. Three of the five mutants with insertions into non-coding regions grew well in deer. Transposition into a differentially expressed hypothetical gene, Ech_0379, and at 18 nucleotides downstream to Ech_0230 gene coding sequence resulted in the inhibition of growth in deer, which is further evidenced by their failed acquisition by ticks. Similarly, a mutation into the coding region of ECH_0660 gene inhibited the in vivo growth in deer. This is the first study evaluating targeted and random mutagenesis in E. chaffeensis, and the first to report the generation of stable mutants in this obligate intracellular bacterium. We further demonstrate that in vitro mutagenesis coupled with in vivo infection assessment is a

  11. Targeted and random mutagenesis of Ehrlichia chaffeensis for the identification of genes required for in vivo infection.

    Directory of Open Access Journals (Sweden)

    Chuanmin Cheng

    2013-02-01

    Full Text Available Ehrlichia chaffeensis is a tick transmitted pathogen responsible for the disease human monocytic ehrlichiosis. Research to elucidate gene function in rickettsial pathogens is limited by the lack of genetic manipulation methods. Mutational analysis was performed, targeting to specific and random insertion sites within the bacterium's genome. Targeted mutagenesis at six genomic locations by homologous recombination and mobile group II intron-based methods led to the consistent identification of mutants in two genes and in one intergenic site; the mutants persisted in culture for 8 days. Three independent experiments using Himar1 transposon mutagenesis of E. chaffeensis resulted in the identification of multiple mutants; these mutants grew continuously in macrophage and tick cell lines. Nine mutations were confirmed by sequence analysis. Six insertions were located within non-coding regions and three were present in the coding regions of three transcriptionally active genes. The intragenic mutations prevented transcription of all three genes. Transposon mutants containing a pool of five different insertions were assessed for their ability to infect deer and subsequent acquisition by Amblyomma americanum ticks, the natural reservoir and vector, respectively. Three of the five mutants with insertions into non-coding regions grew well in deer. Transposition into a differentially expressed hypothetical gene, Ech_0379, and at 18 nucleotides downstream to Ech_0230 gene coding sequence resulted in the inhibition of growth in deer, which is further evidenced by their failed acquisition by ticks. Similarly, a mutation into the coding region of ECH_0660 gene inhibited the in vivo growth in deer. This is the first study evaluating targeted and random mutagenesis in E. chaffeensis, and the first to report the generation of stable mutants in this obligate intracellular bacterium. We further demonstrate that in vitro mutagenesis coupled with in vivo infection

  12. Identification of a Novel Conjugative Plasmid in Mycobacteria That Requires Both Type IV and Type VII Secretion

    KAUST Repository

    Ummels, R.

    2014-09-23

    Conjugative plasmids have been identified in a wide variety of different bacteria, ranging from proteobacteria to firmicutes, and conjugation is one of the most efficient routes for horizontal gene transfer. The most widespread mechanism of plasmid conjugation relies on different variants of the type IV secretion pathway. Here, we describe the identification of a novel type of conjugative plasmid that seems to be unique for mycobacteria. Interestingly, while this plasmid is efficiently exchanged between different species of slow-growing mycobacteria, including Mycobacterium tuberculosis, it could not be transferred to any of the fast-growing mycobacteria tested. Genetic analysis of the conjugative plasmid showed the presence of a locus containing homologues of three type IV secretion system components and a relaxase. In addition, a new type VII secretion locus was present. Using transposon insertion mutagenesis, we show that in fact both these secretion systems are essential for conjugation, indicating that this plasmid represents a new class of conjugative plasmids requiring two secretion machineries. This plasmid could form a useful new tool to exchange or introduce DNA in slow-growing mycobacteria. IMPORTANCE: Conjugative plasmids play an important role in horizontal gene transfer between different bacteria and, as such, in their adaptation and evolution. This effect is most obvious in the spread of antibiotic resistance genes. Thus far, conjugation of natural plasmids has been described only rarely for mycobacterial species. In fact, it is generally accepted that M. tuberculosis does not show any recent sign of horizontal gene transfer. In this study, we describe the identification of a new widespread conjugative plasmid that can also be efficiently transferred to M. tuberculosis. This plasmid therefore poses both a threat and an opportunity. The threat is that, through the acquisition of antibiotic resistance markers, this plasmid could start a rapid spread of

  13. Is macroporosity absolutely required for preliminary in vitro bone biomaterial study? A comparison between porous materials and flat materials.

    Science.gov (United States)

    Lee, Juliana T Y; Chow, King L; Wang, Kefeng; Tsang, Wai-Hung

    2011-11-08

    Porous materials are highly preferred for bone tissue engineering due to space for blood vessel ingrowth, but this may introduce extra experimental variations because of the difficulty in precise control of porosity. In order to decide whether it is absolutely necessary to use porous materials in in vitro comparative osteogenesis study of materials with different chemistries, we carried out osteoinductivity study using C3H/10T1/2 cells, pluripotent mesenchymal stem cells (MSCs), on seven material types: hydroxyapatite (HA), α-tricalcium phosphate (α-TCP) and b-tricalcium phosphate (β-TCP) in both porous and dense forms and tissue culture plastic. For all materials under test, dense materials give higher alkaline phosphatase gene (Alp) expression compared with porous materials. In addition, the cell density effects on the 10T1/2 cells were assessed through alkaline phosphatase protein (ALP) enzymatic assay. The ALP expression was higher for higher initial cell plating density and this explains the greater osteoinductivity of dense materials compared with porous materials for in vitro study as porous materials would have higher surface area. On the other hand, the same trend of Alp mRNA level (HA > β-TCP > α-TCP) was observed for both porous and dense materials, validating the use of dense flat materials for comparative study of materials with different chemistries for more reliable comparison when well-defined porous materials are not available. The avoidance of porosity variation would probably facilitate more reproducible results. This study does not suggest porosity is not required for experiments related to bone regeneration application, but emphasizes that there is often a tradeoff between higher clinical relevance, and less variation in a less complex set up, which facilitates a statistically significant conclusion. Technically, we also show that the base of normalization for ALP activity may influence the conclusion and there may be ALP activity from

  14. Identification of factors required for meristem function in Arabidopsis using a novel next generation sequencing fast forward genetics approach

    Directory of Open Access Journals (Sweden)

    Scheres Ben

    2011-05-01

    Full Text Available Abstract Background Phenotype-driven forward genetic experiments are powerful approaches for linking phenotypes to genomic elements but they still involve a laborious positional cloning process. Although sequencing of complete genomes now becomes available, discriminating causal mutations from the enormous amounts of background variation remains a major challenge. Method To improve this, we developed a universal two-step approach, named 'fast forward genetics', which combines traditional bulk segregant techniques with targeted genomic enrichment and next-generation sequencing technology Results As a proof of principle we successfully applied this approach to two Arabidopsis mutants and identified a novel factor required for stem cell activity. Conclusion We demonstrated that the 'fast forward genetics' procedure efficiently identifies a small number of testable candidate mutations. As the approach is independent of genome size, it can be applied to any model system of interest. Furthermore, we show that experiments can be multiplexed and easily scaled for the identification of multiple individual mutants in a single sequencing run.

  15. Preliminary hazards analysis -- vitrification process

    Energy Technology Data Exchange (ETDEWEB)

    Coordes, D.; Ruggieri, M.; Russell, J.; TenBrook, W.; Yimbo, P. [Science Applications International Corp., Pleasanton, CA (United States)

    1994-06-01

    This paper presents a Preliminary Hazards Analysis (PHA) for mixed waste vitrification by joule heating. The purpose of performing a PHA is to establish an initial hazard categorization for a DOE nuclear facility and to identify those processes and structures which may have an impact on or be important to safety. The PHA is typically performed during and provides input to project conceptual design. The PHA is then followed by a Preliminary Safety Analysis Report (PSAR) performed during Title 1 and 2 design. The PSAR then leads to performance of the Final Safety Analysis Report performed during the facility`s construction and testing. It should be completed before routine operation of the facility commences. This PHA addresses the first four chapters of the safety analysis process, in accordance with the requirements of DOE Safety Guidelines in SG 830.110. The hazards associated with vitrification processes are evaluated using standard safety analysis methods which include: identification of credible potential hazardous energy sources; identification of preventative features of the facility or system; identification of mitigative features; and analyses of credible hazards. Maximal facility inventories of radioactive and hazardous materials are postulated to evaluate worst case accident consequences. These inventories were based on DOE-STD-1027-92 guidance and the surrogate waste streams defined by Mayberry, et al. Radiological assessments indicate that a facility, depending on the radioactive material inventory, may be an exempt, Category 3, or Category 2 facility. The calculated impacts would result in no significant impact to offsite personnel or the environment. Hazardous materials assessment indicates that a Mixed Waste Vitrification facility will be a Low Hazard facility having minimal impacts to offsite personnel and the environment.

  16. Identification of genes required for growth of Escherichia coli MG1655 at moderately low pH

    Directory of Open Access Journals (Sweden)

    Bram Vivijs

    2016-10-01

    Full Text Available The survival of some pathotypes of E. coli in very low pH environments like highly acidic foods and the stomach has been well documented and contributes to their success as foodborne pathogens. In contrast, the ability of E. coli to grow at moderately low pH has received less attention, although this property can be anticipated to be also very important for the safety of mildly acidic foods. Therefore, the objective of this study was to identify cellular functions required for growth of the non-pathogenic strain E. coli MG1655 at low pH. First, the role of the four E. coli amino acid decarboxylase systems, which are the major cellular mechanisms allowing extreme acid survival, was investigated using mutants defective in each of the systems. Only the lysine decarboxylase (CadA was required for low pH growth. Secondly, a screening of 8544 random transposon insertion mutants resulted in the identification of six genes affecting growth in LB broth acidified to pH 4.50 with HCl. Two of the genes, encoding the transcriptional regulator LeuO and the elongation factor P-β-lysine ligase EpmA, can be linked to CadA production. Two other genes, encoding the diadenosine tetraphosphatase ApaH and the tRNA modification GTPase MnmE, have been previously implicated in the bacterial response to stresses other than low pH. A fifth gene encodes the LPS heptosyltransferase WaaC, and its mutant has a deep rough colony phenotype, which has been linked to reduced acid tolerance in earlier work. Finally, tatC encodes a secA-independent protein translocase that exports a few dozen proteins and thus is likely to have a pleiotropic phenotype. For mnmE, apaH, epmA,and waaC, de novo in frame deletion and genetic complementation confirmed their role in low pH growth, and these deletion mutants were also affected in growth in apple juice and tomato juice. However, the mutants were not affected in survival in gastric simulation medium at pH 2.5, indicating that growth at

  17. Protein characterization by LC-MS/MS may be required for the DNA identification of a fusion hemoglobin: the example of Hb P-Nilotic.

    Science.gov (United States)

    Zanella-Cleon, Isabelle; Delolme, Frédéric; Lacan, Philippe; Garcia, Caroline; Vinatier, Isabelle; Francina, Alain; Joly, Philippe

    2012-02-01

    DNA analysis is currently the easiest way to identify a hemoglobin variant in most cases. Nevertheless, in case of complex gene rearrangements, mass spectrometry studies may be required to orientate the DNA diagnosis. The present report shows the use of mass spectrometry techniques prior to DNA analysis for the identification of the rare P-Nilotic fusion hemoglobin. Complete protein analysis is performed by liquid chromatography-tandem mass spectrometry on the abnormal globin chain isolated by reversed-phase liquid chromatography.

  18. "PolyMin": software for identification of the minimum number of polymorphisms required for haplotype and genotype differentiation

    DEFF Research Database (Denmark)

    Frei, Ursala K; Wollenweber, Bernd; Lübberstedt, Thomas

    2009-01-01

    . To date, software for identification of the minimum number of required markers has been optimized for human genetics and is only partly matching the needs of plant scientists and breeders. In addition, different software packages with insufficient interoperability need to be combined to extract...... this information from available allele sequence data, resulting in an errorprone multi-step process of data handling. Results: PolyMin, a computer program combining the detection of a minimum set of single nucleotide polymorphisms (SNPs) and/or insertions/deletions (INDELs) necessary for allele differentiation...

  19. High-level waste storage tank farms/242-A evaporator Standards/Requirements Identification Document (S/RID), Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The High-Level Waste Storage Tank Farms/242-A Evaporator Standards/Requirements Document (S/RID) is contained in multiple volumes. This document (Volume 2) presents the standards and requirements for the following sections: Quality Assurance, Training and Qualification, Emergency Planning and Preparedness, and Construction.

  20. High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1

    Science.gov (United States)

    Schultz, Zachery D.; Warrick, Jay W.; Guckenberger, David J.; Pezzi, Hannah M.; Sperger, Jamie M.; Heninger, Erika; Saeed, Anwaar; Leal, Ticiana; Mattox, Kara; Traynor, Anne M.; Campbell, Toby C.; Berry, Scott M.; Beebe, David J.; Lang, Joshua M.

    2016-01-01

    Background Expression of programmed-death ligand 1 (PD-L1) in non-small cell lung cancer (NSCLC) is typically evaluated through invasive biopsies; however, recent advances in the identification of circulating tumor cells (CTCs) may be a less invasive method to assay tumor cells for these purposes. These liquid biopsies rely on accurate identification of CTCs from the diverse populations in the blood, where some tumor cells share characteristics with normal blood cells. While many blood cells can be excluded by their high expression of CD45, neutrophils and other immature myeloid subsets have low to absent expression of CD45 and also express PD-L1. Furthermore, cytokeratin is typically used to identify CTCs, but neutrophils may stain non-specifically for intracellular antibodies, including cytokeratin, thus preventing accurate evaluation of PD-L1 expression on tumor cells. This holds even greater significance when evaluating PD-L1 in epithelial cell adhesion molecule (EpCAM) positive and EpCAM negative CTCs (as in epithelial-mesenchymal transition (EMT)). Methods To evaluate the impact of CTC misidentification on PD-L1 evaluation, we utilized CD11b to identify myeloid cells. CTCs were isolated from patients with metastatic NSCLC using EpCAM, MUC1 or Vimentin capture antibodies and exclusion-based sample preparation (ESP) technology. Results Large populations of CD11b+CD45lo cells were identified in buffy coats and stained non-specifically for intracellular antibodies including cytokeratin. The amount of CD11b+ cells misidentified as CTCs varied among patients; accounting for 33–100% of traditionally identified CTCs. Cells captured with vimentin had a higher frequency of CD11b+ cells at 41%, compared to 20% and 18% with MUC1 or EpCAM, respectively. Cells misidentified as CTCs ultimately skewed PD-L1 expression to varying degrees across patient samples. Conclusions Interfering myeloid populations can be differentiated from true CTCs with additional staining criteria

  1. High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1.

    Directory of Open Access Journals (Sweden)

    Jennifer L Schehr

    Full Text Available Expression of programmed-death ligand 1 (PD-L1 in non-small cell lung cancer (NSCLC is typically evaluated through invasive biopsies; however, recent advances in the identification of circulating tumor cells (CTCs may be a less invasive method to assay tumor cells for these purposes. These liquid biopsies rely on accurate identification of CTCs from the diverse populations in the blood, where some tumor cells share characteristics with normal blood cells. While many blood cells can be excluded by their high expression of CD45, neutrophils and other immature myeloid subsets have low to absent expression of CD45 and also express PD-L1. Furthermore, cytokeratin is typically used to identify CTCs, but neutrophils may stain non-specifically for intracellular antibodies, including cytokeratin, thus preventing accurate evaluation of PD-L1 expression on tumor cells. This holds even greater significance when evaluating PD-L1 in epithelial cell adhesion molecule (EpCAM positive and EpCAM negative CTCs (as in epithelial-mesenchymal transition (EMT.To evaluate the impact of CTC misidentification on PD-L1 evaluation, we utilized CD11b to identify myeloid cells. CTCs were isolated from patients with metastatic NSCLC using EpCAM, MUC1 or Vimentin capture antibodies and exclusion-based sample preparation (ESP technology.Large populations of CD11b+CD45lo cells were identified in buffy coats and stained non-specifically for intracellular antibodies including cytokeratin. The amount of CD11b+ cells misidentified as CTCs varied among patients; accounting for 33-100% of traditionally identified CTCs. Cells captured with vimentin had a higher frequency of CD11b+ cells at 41%, compared to 20% and 18% with MUC1 or EpCAM, respectively. Cells misidentified as CTCs ultimately skewed PD-L1 expression to varying degrees across patient samples.Interfering myeloid populations can be differentiated from true CTCs with additional staining criteria, thus improving the

  2. Preliminary planning study for safety relief valve experiments in a Mark III BWR pressure suppression system

    Energy Technology Data Exchange (ETDEWEB)

    McCauley, E.W.; Holman, G.S.

    1980-04-21

    In response to a request from the Water Reactor Safety Research Division of the US NRC, a preliminary study is provided which identifies key features and consideration involved in planning a comprehensive in-plant Safety Relief Valve experimental program for a Mark III containment design. The report provides identification of program objectives, measurement system requirements, and some details quantifying expected system response. In addition, a preliminary test matrix is outlined which involves a supporting philosophy intended to enhance the usefulness of the experimental results for all members of the program team: experimentalists, analysts, and plant operator.

  3. IDENTIFICATION OF MEASUREMENT ITEMS OF DESIGN REQUIREMENTS FOR LEAN AND AGILE SUPPLY CHAIN-CONFIRMATORY FACTOR ANALYSIS

    Directory of Open Access Journals (Sweden)

    D.Venkata Ramana

    2013-06-01

    Full Text Available This study examines the consistency approaches by confirmatory factor analysis that determines the construct validity, convergent validity, construct reliability and internal consistency of the items of strategic design requirements. The design requirements includes use of information technology, sourcing procedures, new product development, flexible manufacturing functions and demand management supply chain net work design, management, commitment and inventory management policies among manufacturers of volatile and unforeseeable products in Andhraadesh, India. This study suggested that the seven factor model with 20 items of the leagile supply chain design requirements had a good fit. Further, the study showed a val id and reliable measurement to identify critical items among the design requirements of leagile supply chains.

  4. Image enhancements of Landsat 8 (OLI) and SAR data for preliminary landslide identification and mapping applied to the central region of Kenya

    Science.gov (United States)

    Mwaniki, M. W.; Kuria, D. N.; Boitt, M. K.; Ngigi, T. G.

    2017-04-01

    Image enhancements lead to improved performance and increased accuracy of feature extraction, recognition, identification, classification and hence change detection. This increases the utility of remote sensing to suit environmental applications and aid disaster monitoring of geohazards involving large areas. The main aim of this study was to compare the effect of image enhancement applied to synthetic aperture radar (SAR) data and Landsat 8 imagery in landslide identification and mapping. The methodology involved pre-processing Landsat 8 imagery, image co-registration, despeckling of the SAR data, after which Landsat 8 imagery was enhanced by Principal and Independent Component Analysis (PCA and ICA), a spectral index involving bands 7 and 4, and using a False Colour Composite (FCC) with the components bearing the most geologic information. The SAR data were processed using textural and edge filters, and computation of SAR incoherence. The enhanced spatial, textural and edge information from the SAR data was incorporated to the spectral information from Landsat 8 imagery during the knowledge based classification. The methodology was tested in the central highlands of Kenya, characterized by rugged terrain and frequent rainfall induced landslides. The results showed that the SAR data complemented Landsat 8 data which had enriched spectral information afforded by the FCC with enhanced geologic information. The SAR classification depicted landslides along the ridges and lineaments, important information lacking in the Landsat 8 image classification. The success of landslide identification and classification was attributed to the enhanced geologic features by spectral, textural and roughness properties.

  5. Preliminary Remarks Regarding the Prevalence of ESBL-Producing Strains of E. coli and K. Pneumoniae, Isolated from Cows with Clinical Endometritis

    Directory of Open Access Journals (Sweden)

    Cristina Ioana CRIVEI

    2017-05-01

    In this preliminary study, by phenotypic methods was confirmed a prevalence of 35.3% for the ESBL strains of E. coli and K. pneumoniae, which requires further research to confirm by molecular biology the identification of ESBL resistance genes, but also for the plasmids encoding these gene transmission.

  6. 45 CFR 150.217 - Preliminary determination.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Preliminary determination. 150.217 Section 150.217... Are Failing To Substantially Enforce HIPAA Requirements § 150.217 Preliminary determination. If, at... designees). (b) Notifies the State of CMS's preliminary determination that the State has failed to...

  7. 40 CFR 91.113 - Requirement of certification-emission control information label and engine identification number.

    Science.gov (United States)

    2010-07-01

    ... MARINE SPARK-IGNITION ENGINES Emission Standards and Certification Provisions § 91.113 Requirement of... during engine life; (4) Be written in English; and (5) Be located so as to be readily visible to the average person after the engine is installed in the marine vessel. (b) If the marine vessel obscures...

  8. Teaching Case: IS Security Requirements Identification from Conceptual Models in Systems Analysis and Design: The Fun & Fitness, Inc. Case

    Science.gov (United States)

    Spears, Janine L.; Parrish, James L., Jr.

    2013-01-01

    This teaching case introduces students to a relatively simple approach to identifying and documenting security requirements within conceptual models that are commonly taught in systems analysis and design courses. An introduction to information security is provided, followed by a classroom example of a fictitious company, "Fun &…

  9. Teaching Case: IS Security Requirements Identification from Conceptual Models in Systems Analysis and Design: The Fun & Fitness, Inc. Case

    Science.gov (United States)

    Spears, Janine L.; Parrish, James L., Jr.

    2013-01-01

    This teaching case introduces students to a relatively simple approach to identifying and documenting security requirements within conceptual models that are commonly taught in systems analysis and design courses. An introduction to information security is provided, followed by a classroom example of a fictitious company, "Fun &…

  10. Preliminary identification of hemoglobin q-iran in an Iranian family from central province of Iran by globin chain analysis on HPLC.

    Science.gov (United States)

    Khatami, Shohreh; Najmabadi, Hossein; Rouhi, Soghra; Mirzazadeh, Roghieh; Bayat, Parastoo; Sadeghi, Sedigheh

    2013-12-01

    Many abnormal α-chain hemoglobins (Hbs) are caused by single nucleotide mutations in α1- or α2-goblin genes. One of these Hbs is Hb Q-Iran which is resulted from a point mutation at codon 75 of the α1-globin gene (Asp→His). The identification of Hb Q-Iran was observed in two members of a family from the Central Province of Iran. In this study, Globin chain analysis on high performance liquid chromatography (HPLC) and DNA sequencing were applied. An unusual Hb variant, like HbS on alkaline pH electrophoresis was identified from samples of a father and his son from Arak city in the Central Province of Iran. The variant was further characterized by globin chain analysis and DNA sequencing methods. Globin chain analysis revealed an unknown globin chain peak after α-globin chain peak with a different retention time from βs-globin chain, as the control in both samples. Genetic analysis led to the identification of an unknown Hb variant, Hb Q-Iran. Globin chain analysis showed the presence of an unknown globin chain, and likewise DNA sequencing revealed HbQ-Iran. In other words, Globin chain analysis procedure could preliminarily detect an unknown globin chain.

  11. Identification and preliminary characterization of global water resource issues which may be affected by CO/sub 2/-induced climate change

    Energy Technology Data Exchange (ETDEWEB)

    Callaway, J.M.; Cohen, M.L.; Currie, J.W.

    1984-04-01

    The objectives were to: (1) identify, characterize, and define existing or projected regional and global water resource management issues which may be affected by CO/sub 2/-induced climate changes; and (2) develop research priorities for acquiring additional information about the potential effects of a CO/sub 2/-induced climate change on the availability and allocation of freshwater supplies. The research was broken into four work elements: (1) identification of water resource management issues on a global and regional basis; (2) identification of a subset of generic CO/sub 2/-related water resource management issues believed to have the highest probability of being affected, beneficially or adversely, by a CO/sub 2/-induced climate change; (3) selection of specific sites for examining the potential effect of a CO/sub 2/-induced climate change on these issues; and (4) conducting detailed case studies at these sites, the results from which will be used to identify future research and data needs in the area of water resources. This report summarizes the research related to the first three work elements. 6 figures, 9 tables.

  12. Requirements Identification Towards a Design of Adaptive ICTs for Supporting Bipolar Disorder Treatment in Different Healthcare Contexts

    Directory of Open Access Journals (Sweden)

    Emanuele Torri

    2015-10-01

    Full Text Available This paper presents patient and caregiver perspective on ICTs supporting bipolar disorder management in multinational healthcare provisioning contexts. The envisioned mHealth solutions should adopt general requirements that could be instantiated into different clinical settings. Engagement of users in designing new technologies for mental health is crucial to ensure empowerment and patient-centeredness of services. We performed focus groups to understand user needs, attitudes and experiences towards the supportive ICTs in two target regions where the expected solutions will operate. The survey offered valuable inputs for the construction of the clinical requirements used to produce a trans-national call for tender on mobile health solutions aimed at supporting bipolar disorders treatment among public purchasers in different European countries. The study was part of the NYMHPA-MD (Next Generation Mobile Platform for Health in Mental Disorders project, co-funded by the European Commission.

  13. Glutathione S-transferase Ya subunit gene: identification of regulatory elements required for basal level and inducible expression.

    OpenAIRE

    Telakowski-Hopkins, C A; King, R. G.; Pickett, C B

    1988-01-01

    The function of the 5'-flanking region of a rat glutathione S-transferase Ya subunit structural gene has been examined in homologous and heterologous cells. By using the 5'-flanking region of the Ya subunit gene fused to the structural gene encoding chloramphenicol acetyltransferase, we have identified two cis-acting regulatory elements in the upstream region of the Ya gene. One element is required for maximum basal level expression in homologous cells, whereas maximum basal level expression ...

  14. Microarray Analysis of Transposon Insertion Mutants in Bacillus Anthracis: Global Identification of Genes Required for Sporulation and Germination

    Science.gov (United States)

    2007-02-01

    Gilois, M. Rose, and D. Lereclus. 2001. Oligopep- tide permease is required for expression of the Bacillus thuringiensis plcR regulon and for...non- toxin gene expression in Bacillus anthracis. Infect. Immun. 65:3091–3099. 10. Ikeda, R. A., C. M. Ligman, and S. Warshamana. 1992. T7 promoter con...nontoxi- genic Bacillus anthracis spore vaccines based on strains expressing mutant vari- ants of lethal toxin components. Vaccine 23:5688–5697. 17. Read

  15. Water budgets and groundwater volumes for abandoned underground mines in the Western Middle Anthracite Coalfield, Schuylkill, Columbia, and Northumberland Counties, Pennsylvania-Preliminary estimates with identification of data needs

    Science.gov (United States)

    Goode, Daniel J.; Cravotta, Charles A.; Hornberger, Roger J.; Hewitt, Michael A.; Hughes, Robert E.; Koury, Daniel J.; Eicholtz, Lee W.

    2011-01-01

    levels and stream base flow, the latter at many locations composed primarily of discharge from mines. Automatic parameter estimation used MODFLOW-2000 with manual adjustments to constrain parameter values to realistic ranges. The calibrated model supports the conceptual model of high-permeability MCUs separated by low-permeability barriers and streamflow losses and gains associated with mine infiltration and discharge. The simulated groundwater levels illustrate low groundwater gradients within an MCU and abrupt changes in water levels between MCUs. The preliminary model results indicate that the primary result of increased pumping from the mine would be reduced discharge from the mine to streams near the pumping wells. The intact barriers limit the spatial extent of mine dewatering. Considering the simulated groundwater levels, depth of mining, and assumed bulk porosity of 11 or 40 percent for the mined seams, the water volume in storage in the mines of the Western Middle Anthracite Coalfield was estimated to range from 60 to 220 billion gallons, respectively. Details of the groundwater-level distribution and the rates of some mine discharges are not simulated well using the preliminary model. Use of the model results should be limited to evaluation of the conceptual model and its simulation using porous-media flow methods, overall water budgets for the Western Middle Anthracite Coalfield, and approximate storage volumes. Model results should not be considered accurate for detailed simulation of flow within a single MCU or individual flooded mine. Although improvements in the model calibration were possible by introducing spatial variability in permeability parameters and adjusting barrier properties, more detailed parameterizations have increased uncertainty because of the limited data set. The preliminary identification of data needs includes continuous streamflow, mine discharge rate, and groundwater levels in the mines and adjacent areas. Data collected whe

  16. Proposal of how to update the standard information requirements in REACH, PPPR and BPR – a testing strategy for identification of endocrine disruptors

    DEFF Research Database (Denmark)

    Holbech, Henrik; Bjerregaard, Poul; Hass, Ulla

    This presentation is based on a project prepared by the Danish Centre on Endocrine Disrupters (CEHOS) for the Danish EPA. The project aim to provide a science based input to the on-going work in EU with regard to endocrine disruptors, i.e. the development of criteria for identification, REACH...... review on EDs and the revised strategy for the future work on endocrine disruptors, focusing on adequate detection of substances with endocrine disrupting properties under various legislative frameworks, including REACH (EC No 1907/2006), the Plant Protection Products Regulation (PPPR) (EC No 1107....../2009) and the Biocidal Products Regulation (BPR) (EC No 528/2012). There are currently no specific information requirements or testing strategies with regard to endocrine disruption in REACH and other relevant legislations. However, in relation to biocides and recently also to plant protection products, indications...

  17. Identification of co-existing cationic surfactants with preliminary separation on silica HPTLC plates using mixed aqueous sodium chloride-ethanol as eluent

    Directory of Open Access Journals (Sweden)

    Ali Mohammad

    2015-12-01

    Full Text Available A new high-performance thin-layer chromatographic system comprising of silica gel 60 HPTLC plates as stationary phase and ethanol-5% aqueous sodium chloride (8:2 as mobile phase has been identified as most suitable for separation of quaternary mixture of cationic surfactants. Separation efficacy of developed method has been established by obtaining well-resolved densitogram of separated spots. To check the versatility, effects of presence of metal cations, inorganic anions, amino acids, vitamins and non-ionic surfactants as impurities were also examined. The chromatographic parameters like ΔRF, separation factor (α, resolution (RS and limit of detection were also calculated. The proposed method is applicable for the identification of surfactants in eye drops and Colgate Plax mouthwash.

  18. Identification and preliminary characterization of non-polyene antibiotics secreted by new strain of actinomycete isolated from sebkha of Kenadsa, Algeria

    Institute of Scientific and Technical Information of China (English)

    Omar; Messaoudi; Mourad; Bendahou; Ibrahim; Benamar; Djamal-Elddine; Abdelwouhid

    2015-01-01

    Objective: To study the antimicrobial activity of actinomycetes isolated from sabkha of Kenadsa and identification of the isolate interesting. Methods: Eighteen strains were isolated, using four culture media from sebkha of Kenadsa(Bechar, Southwestern Algeria). Screening of antimicrobial activity consisted of two steps: in primary screening, antibacterial activity was determined by using the agar plug method against test strains; in secondary screening, better isolate which showed a good activity in the first screening was selected to extract antimicrobial substances. The antimicrobial activities of extracts were evaluated by using Kirby-Bauer disc diffusion method. Partial characterization of antimicrobial products was performed on the basis of chemical revelations, UV-vis spectrometry and infrared spectroscopy. The identification of isolate interesting was performed through morphological, chemical, biochemical and physiological characteristics. Results: All isolates showed antimicrobial activity against at least one microorganism test. One isolate, LAM143 cG 3, was selected for its broad spectrum and high antimicrobial activity. The isolate LAM143 c G3 was identified as Spirillospora sp. The comparison between the species of this genus(Spirillospora rubra and Spirillospora albida) and our isolate indicated the existence of several physiological and biochemical differences which led us to suppose that this was a new member of this genus. Primary characterization of antimicrobial substances produced by the isolate LAM143 c G3 indicated the presence of amines and phenols. The UV–vis spectrum suggested a non-polyenic nature of substances secreted by our isolate, while infrared confirmed the presence of amine groups.Conclusions: The result of the present study revealed that sebkha of Kenadsa was rich in rare actinomycetes, that secreted interesting antimicrobial substance.

  19. Regulation of carotenoid and bacteriochlorophyll biosynthesis genes and identification of an evolutionarily conserved gene required for bacteriochlorophyll accumulation.

    Science.gov (United States)

    Armstrong, G A; Cook, D N; Ma, D; Alberti, M; Burke, D H; Hearst, J E

    1993-05-01

    The temporal expression of ten clustered genes required for carotenoid (crt) and bacteriochlorophyll (bch) biosynthesis was examined during the transition from aerobic respiration to anaerobiosis requisite for the development of the photosynthetic membrane in the bacterium Rhodobacter capsulatus. Accumulation of crtA, crtC, crtD, crtE, crtF, crtK, bchC and bchD mRNAs increased transiently and coordinately, up to 12-fold following removal of oxygen from the growth medium, paralleling increases in mRNAs encoding pigment-binding polypeptides of the photosynthetic apparatus. The crtB and crtI genes, in contrast, were expressed similarly in the presence or absence of oxygen. The regulation patterns of promoters for the crtA and crtI genes and the bchCXYZ operon were characterized using lacZ transcriptional fusion and qualitatively reflected the corresponding mRNA accumulation patterns. We also report that the bchI gene product, encoded by a DNA sequence previously considered to be a portion of crtA, shares 49% sequence identity with the nuclear-encoded Arabidopsis thaliana Cs chloroplast protein required for normal pigmentation in plants.

  20. Identification of the optimal structure required for a Shiga toxin neutralizer with oriented carbohydrates to function in the circulation.

    Science.gov (United States)

    Nishikawa, Kiyotaka; Matsuoka, Koji; Watanabe, Miho; Igai, Katsura; Hino, Kumiko; Hatano, Ken; Yamada, Akihiro; Abe, Nobuhisa; Terunuma, Daiyo; Kuzuhara, Hiroyoshi; Natori, Yasuhiro

    2005-06-15

    Shiga toxin (Stx) is a major virulence factor of Stx-producing Escherichia coli. Recently, we developed a therapeutic Stx neutralizer with 6 trisaccharides of globotriaosyl ceramide, a receptor for Stx, in its dendrimer structure (referred to as "SUPER TWIG [1]6") to function in the circulation. Here, we determined the optimal structure of SUPER TWIG for it to function in the circulation and identified a SUPER TWIG with 18 trisaccharides, SUPER TWIG (2)18, as another potent Stx neutralizer. SUPER TWIGs (1)6 and (2)18 shared a structural similarity, a dumbbell shape in which 2 clusters of trisaccharides were connected via a linkage with a hydrophobic chain. The dumbbell shape was found to be required for formation of a complex with Stx that enables efficient uptake and degradation of Stx by macrophages and, consequently, for potent Stx-neutralizing activity in the circulation. We also determined the binding site of the SUPER TWIGs on Stx.

  1. High-level waste storage tank farms/242-A evaporator standards/requirements identification document (S/RID), Vol. 5

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The Fire Protection functional area for the Hanford Site Tank Farm facilities and support structures is based on the application of relevant DOE orders, regulations, and industry codes and standards. The fire protection program defined in this document may be divided into three areas: (1) organizational, (2) administrative programmatic features, and (3) technical features. The information presented in each section is in the form of program elements and orders, regulations, industry codes, and standards that serve as the attributes of a fire protection program for the Tank Farm facilities. Upon completion this document will be utilized as the basis to evaluate compliance of the fire protection program being implemented for the Tank Farm facilities with the requirements of DOE orders and industry codes and standards.

  2. Preliminary identification of children with acute lymphoblastic leukemia and juvenile idiopathic arthritis%儿童急性淋巴细胞白血病和幼年特发性关节炎的初步鉴别

    Institute of Scientific and Technical Information of China (English)

    李琛(综述); 曹兰芳; 薛海燕(审校)

    2014-01-01

    部分急性淋巴细胞白血病患儿以骨骼肌肉表现为首发症状,这些患儿中有一部分会被误诊为幼年特发性关节炎,如何早期区分这些患儿对于及时治疗、改善预后很有意义。该文根据病史及常规的实验室检查和影像学检查,提出在疾病早期如何通过分析有关节症状患儿关节肿痛、血象及影像学特点初步鉴别儿童急性淋巴细胞白血病和幼年特发性关节炎,降低误诊率。%With acute lymphoblastic leukemia in skeletal muscle symptom,part of these patients may be misdiagnosed as juvenile idiopathic arthritis. How to distinguish these children has significance for the timely and proper treatment and good prognosis. This article from the history and routine laboratory examination and ima-ging examination put forward early in the disease through the analysis of joint symptoms from juvenile joint pain,blood and imaging characteristics and preliminary identification of childhood leukemia from juvenile idio-pathic arthritis in order to decrease the rate of misdiagnosis.

  3. Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritima

    Science.gov (United States)

    Larrea, Andres A.; Pedroso, Ilene M.; Malhotra, Arun; Myers, Richard S.

    2008-01-01

    Exonuclease VII was first identified in 1974 as a DNA exonuclease that did not require any divalent cations for activity. Indeed, Escherichia coli ExoVII was identified in partially purified extracts in the presence of EDTA. ExoVII is comprised of two subunits (XseA and XseB) that are highly conserved and present in most sequenced prokaryotic genomes, but are not seen in eukaryotes. To better understand this exonuclease family, we have characterized an ExoVII homolog from Thermotoga maritima. Thermotoga maritima XseA/B homologs TM1768 and TM1769 were co-expressed and purified, and show robust nuclease activity at 80°C. This activity is magnesium dependent and is inhibited by phosphate ions, which distinguish it from E. coli ExoVII. Nevertheless, both E. coli and T. maritima ExoVII share a similar putative active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. We show that these residues, Asp235 and Asp240, are essential for the nuclease activity of T. maritima ExoVII. We hypothesize that the ExoVII family of nucleases can be sub-divided into two sub-families based on EDTA resistance and that T. maritima ExoVII is the first member of the branch that is characterized by EDTA sensitivity and inhibition by phosphate. PMID:18812402

  4. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay

    Science.gov (United States)

    Park, Ok Hyun; Park, Joori; Yu, Mira; An, Hyoung-Tae; Ko, Jesang; Kim, Yoon Ki

    2016-01-01

    Glucocorticoid (GC) receptor (GR) has been shown recently to bind a subset of mRNAs and elicit rapid mRNA degradation. However, the molecular details of GR-mediated mRNA decay (GMD) remain unclear. Here, we demonstrate that GMD triggers rapid degradation of target mRNAs in a translation-independent and exon junction complex-independent manner, confirming that GMD is mechanistically distinct from nonsense-mediated mRNA decay (NMD). Efficient GMD requires PNRC2 (proline-rich nuclear receptor coregulatory protein 2) binding, helicase ability, and ATM-mediated phosphorylation of UPF1 (upstream frameshift 1). We also identify two GMD-specific factors: an RNA-binding protein, YBX1 (Y-box-binding protein 1), and an endoribonuclease, HRSP12 (heat-responsive protein 12). In particular, using HRSP12 variants, which are known to disrupt trimerization of HRSP12, we show that HRSP12 plays an essential role in the formation of a functionally active GMD complex. Moreover, we determine the hierarchical recruitment of GMD factors to target mRNAs. Finally, our genome-wide analysis shows that GMD targets a variety of transcripts, implicating roles in a wide range of cellular processes, including immune responses.

  5. Sensitivity of wild type and mutant ras alleles to Ras specific exchange factors: Identification of factor specific requirements.

    Science.gov (United States)

    Nielsen, K H; Gredsted, L; Broach, J R; Willumsen, B M

    2001-04-19

    We have investigated the productive interaction between the four mammalian Ras proteins (H-, N-, KA- and KB-Ras) and their activators, the mammalian exchange factors mSos1, GRF1 and GRP, by using a modified Saccharomyces cerevisiae whose growth is dependent on activation of a mammalian Ras protein by its activator. All four mammalian Ras proteins were activated with similar efficiencies by the individual exchange factors. The H-Ras mutant V103E, which is competent for membrane localization, nucleotide binding, intrinsic and stimulated GTPase activity as well as intrinsic exchange, was defective for activation by all factors tested, suggesting that the integrity of this residue is necessary for catalyzed exchange. However, when other H-Ras mutants were studied, some distinct sensitivities to the exchange factors were observed. GRP-mediated, but not mSos1-mediated, exchange was blocked in additional mutants, suggesting different structural requirements for GRP. Analysis of Ras-mediated gene activation in murine fibroblasts confirmed these results.

  6. Identification of DSB-1, a protein required for initiation of meiotic recombination in Caenorhabditis elegans, illuminates a crossover assurance checkpoint.

    Directory of Open Access Journals (Sweden)

    Ericca L Stamper

    Full Text Available Meiotic recombination, an essential aspect of sexual reproduction, is initiated by programmed DNA double-strand breaks (DSBs. DSBs are catalyzed by the widely-conserved Spo11 enzyme; however, the activity of Spo11 is regulated by additional factors that are poorly conserved through evolution. To expand our understanding of meiotic regulation, we have characterized a novel gene, dsb-1, that is specifically required for meiotic DSB formation in the nematode Caenorhabditis elegans. DSB-1 localizes to chromosomes during early meiotic prophase, coincident with the timing of DSB formation. DSB-1 also promotes normal protein levels and chromosome localization of DSB-2, a paralogous protein that plays a related role in initiating recombination. Mutations that disrupt crossover formation result in prolonged DSB-1 association with chromosomes, suggesting that nuclei may remain in a DSB-permissive state. Extended DSB-1 localization is seen even in mutants with defects in early recombination steps, including spo-11, suggesting that the absence of crossover precursors triggers the extension. Strikingly, failure to form a crossover precursor on a single chromosome pair is sufficient to extend the localization of DSB-1 on all chromosomes in the same nucleus. Based on these observations we propose a model for crossover assurance that acts through DSB-1 to maintain a DSB-permissive state until all chromosome pairs acquire crossover precursors. This work identifies a novel component of the DSB machinery in C. elegans, and sheds light on an important pathway that regulates DSB formation for crossover assurance.

  7. Identification of DSB-1, a protein required for initiation of meiotic recombination in Caenorhabditis elegans, illuminates a crossover assurance checkpoint.

    Science.gov (United States)

    Stamper, Ericca L; Rodenbusch, Stacia E; Rosu, Simona; Ahringer, Julie; Villeneuve, Anne M; Dernburg, Abby F

    2013-01-01

    Meiotic recombination, an essential aspect of sexual reproduction, is initiated by programmed DNA double-strand breaks (DSBs). DSBs are catalyzed by the widely-conserved Spo11 enzyme; however, the activity of Spo11 is regulated by additional factors that are poorly conserved through evolution. To expand our understanding of meiotic regulation, we have characterized a novel gene, dsb-1, that is specifically required for meiotic DSB formation in the nematode Caenorhabditis elegans. DSB-1 localizes to chromosomes during early meiotic prophase, coincident with the timing of DSB formation. DSB-1 also promotes normal protein levels and chromosome localization of DSB-2, a paralogous protein that plays a related role in initiating recombination. Mutations that disrupt crossover formation result in prolonged DSB-1 association with chromosomes, suggesting that nuclei may remain in a DSB-permissive state. Extended DSB-1 localization is seen even in mutants with defects in early recombination steps, including spo-11, suggesting that the absence of crossover precursors triggers the extension. Strikingly, failure to form a crossover precursor on a single chromosome pair is sufficient to extend the localization of DSB-1 on all chromosomes in the same nucleus. Based on these observations we propose a model for crossover assurance that acts through DSB-1 to maintain a DSB-permissive state until all chromosome pairs acquire crossover precursors. This work identifies a novel component of the DSB machinery in C. elegans, and sheds light on an important pathway that regulates DSB formation for crossover assurance.

  8. Analysis of Campylobacter jejuni infection in the gnotobiotic piglet and genome-wide identification of bacterial factors required for infection

    Science.gov (United States)

    de Vries, Stefan P. W.; Linn, Aileen; Macleod, Kareen; MacCallum, Amanda; Hardy, Simon P.; Douce, Gill; Watson, Eleanor; Dagleish, Mark P.; Thompson, Hal; Stevenson, Andy; Kennedy, David; Baig, Abiyad; Coward, Chris; Maskell, Duncan J.; Smith, David G. E.; Grant, Andrew J.; Everest, Paul

    2017-01-01

    To investigate how Campylobacter jejuni causes the clinical symptoms of diarrhoeal disease in humans, use of a relevant animal model is essential. Such a model should mimic the human disease closely in terms of host physiology, incubation period before onset of disease, clinical signs and a comparable outcome of disease. In this study, we used a gnotobiotic piglet model to study determinants of pathogenicity of C. jejuni. In this model, C. jejuni successfully established infection and piglets developed an increased temperature with watery diarrhoea, which was caused by a leaky epithelium and reduced bile re-absorption in the intestines. Further, we assessed the C. jejuni genes required for infection of the porcine gastrointestinal tract utilising a transposon (Tn) mutant library screen. A total of 123 genes of which Tn mutants showed attenuated piglet infection were identified. Our screen highlighted a crucial role for motility and chemotaxis, as well as central metabolism. In addition, Tn mutants of 14 genes displayed enhanced piglet infection. This study gives a unique insight into the mechanisms of C. jejuni disease in terms of host physiology and contributing bacterial factors. PMID:28281647

  9. Identification of genes required for secretion of the Francisella oxidative burst-inhibiting acid phosphatase AcpA

    Directory of Open Access Journals (Sweden)

    John S Gunn

    2016-04-01

    Full Text Available Francisella tularensis is a Tier 1 bioterror threat and the intracellular pathogen responsible for tularemia in humans and animals. Upon entry into the host, Francisella uses multiple mechanisms to evade killing. Our previous studies have shown that after entering its primary cellular host, the macrophage, Francisella immediately suppresses the oxidative burst by secreting a series of acid phosphatases including AcpA-B-C and HapA, thereby evading the innate immune response of the macrophage and enhancing survival and further infection. However, the mechanism of acid phosphatase secretion by Francisella is still unknown. In this study, we screened for genes required for AcpA secretion in Francisella. We initially demonstrated that the known secretion systems, the putative Francisella-pathogenicity island (FPI-encoded Type VI secretion system and the Type IV pili, do not secrete AcpA. Using random transposon mutagenesis in conjunction with ELISA, Western blotting and acid phosphatase enzymatic assays, a transposon library of 5450 mutants was screened for strains with a minimum 1.5-fold decrease in secreted (culture supernatant AcpA, but no defect in cytosolic AcpA. Three mutants with decreased supernatant AcpA were identified. The transposon insertion sites of these mutants were revealed by direct genomic sequencing or inverse-PCR and sequencing. One of these mutants has a severe defect in AcpA secretion (at least 85% decrease and is a predicted hypothetical inner membrane protein. Interestingly, this mutant also affected the secretion of the FPI-encoded protein, VgrG. Thus, this screen identified novel protein secretion factors involved in the subversion of host defenses.

  10. Semi-automatic identification photo generation with facial pose and illumination normalization

    Science.gov (United States)

    Jiang, Bo; Liu, Sijiang; Wu, Song

    2016-07-01

    Identification photo is a category of facial image that has strict requirements on image quality like size, illumination, user expression, dressing, etc. Traditionally, these photos are taken in professional studios. With the rapid popularity of mobile devices, how to conveniently take identification photo at any time and anywhere with such devices is an interesting problem. In this paper, we propose a novel semi-automatic identification photo generation approach. Given a user image, facial pose and expression are first normalized to meet the basic requirements. To correct uneven lighting condition in photo, an facial illumination normalization approach is adopted to further improve the image quality. Finally, foreground user is extracted and re-targeted to a specific photo size. Besides, background can also be changed as required. Preliminary experimental results show that the proposed method is efficient and effective in identification photo generation compared to commercial software based manual tunning.

  11. SNOW COVER OF THE CENTRAL ANTARCTICA (VOSTOK STATION AS AN IDEAL NATURAL TABLET FOR COSMIC DUST COLLECTION: PRELIMINARY RESULTS ON THE IDENTIFICATION OF MICROMETEORITES OF CARBONACEOUS CHONDRITE TYPE

    Directory of Open Access Journals (Sweden)

    E. S. Bulat

    2012-01-01

    Full Text Available During the 2010/11 season nearby the Vostok station the 56th Russian Antarctic Expedition has collected surface snow in a big amount from a 3 m deep pit using 15 220 L vol. containers (about 70 kg snow each. Snow melting and processing by ultra-centrifugation was performed in a clean (class 10 000 and 100 laboratory. Total dust concentrations were not exceeded 37.4 mkg per liter with particle dispersal mode around 2.5 mkm. To analyze the elemental composition of fine dust particles aimed to reveal Antarctic micrometeorites (AMM two electron microscopy devices equipped with different micro-beams were implemented. As a preliminary result, three particles (of 107 analyzed featured by Mg content clearly dominated over Al along with Si and Fe as major elements (a feature of carbonaceous chondrites were observed. By this the Vostok AMM CS11 collection was established. The occurrence of given particles was averaged 2.8% – the factual value obtained for the first time for chondritic type AMM at Vostok which should be considered as the lowest estimate for all other families of AMM. Given the reference profile of total dust content in East Antarctic snow during Holocene (18 mkg/kg the MM deposition in Antarctica was quantified for the first time – 14 tons per day for carbonaceous chondrites for the Vostok AMM CS11 collection and up to 245 tons per day for all MM types for the Concordia AMM DC02 collection. The results obtained allowed to prove that snow cover (ice sheet in total of Central East Antarctica is the best spot (most clean of other natural locations and always below 0 ºC for collecting native MM deposited on the Earth during the last million years and could be useful in deciphering the origin and evolution of solid matter in our Solar System and its effects on Earth-bound biogeochemical and geophysical processes including the life origin. The farther analyses of the Vostok AMMs are in a progress.

  12. Identification of a highly conserved valine-glycine-phenylalanine amino acid triplet required for HIV-1 Nef function

    Directory of Open Access Journals (Sweden)

    Meuwissen Pieter J

    2012-04-01

    Full Text Available Abstract Background The Nef protein of HIV facilitates virus replication and disease progression in infected patients. This role as pathogenesis factor depends on several genetically separable Nef functions that are mediated by interactions of highly conserved protein-protein interaction motifs with different host cell proteins. By studying the functionality of a series of nef alleles from clinical isolates, we identified a dysfunctional HIV group O Nef in which a highly conserved valine-glycine-phenylalanine (VGF region, which links a preceding acidic cluster with the following proline-rich motif into an amphipathic surface was deleted. In this study, we aimed to study the functional importance of this VGF region. Results The dysfunctional HIV group O8 nef allele was restored to the consensus sequence, and mutants of canonical (NL4.3, NA-7, SF2 and non-canonical (B2 and C1422 HIV-1 group M nef alleles were generated in which the amino acids of the VGF region were changed into alanines (VGF→AAA and tested for their capacity to interfere with surface receptor trafficking, signal transduction and enhancement of viral replication and infectivity. We found the VGF motif, and each individual amino acid of this motif, to be critical for downregulation of MHC-I and CXCR4. Moreover, Nef’s association with the cellular p21-activated kinase 2 (PAK2, the resulting deregulation of cofilin and inhibition of host cell actin remodeling, and targeting of Lck kinase to the trans-golgi-network (TGN were affected as well. Of particular interest, VGF integrity was essential for Nef-mediated enhancement of HIV virion infectivity and HIV replication in peripheral blood lymphocytes. For targeting of Lck kinase to the TGN and viral infectivity, especially the phenylalanine of the triplet was essential. At the molecular level, the VGF motif was required for the physical interaction of the adjacent proline-rich motif with Hck. Conclusion Based on these findings, we

  13. Preliminary identification and coat protein gene phylogenetic relationships of begomoviruses associated with native flora and cultivated plants from the Yucatan Peninsula of Mexico.

    Science.gov (United States)

    Hernández-Zepeda, Cecilia; Idris, Ali M; Carnevali, Germán; Brown, Judith K; Moreno-Valenzuela, Oscar A

    2007-12-01

    A number of native and cultivated eudicots in the Yucatan Peninsula of Mexico (YPM) exhibit symptoms associated with virus infection. Symptomatic leaves were collected and assessed for begomoviral detection using polymerase chain reaction (PCR), and universal primers that amplify a fragment of the coat protein gene (core Cp). Begomovirus were detected in nine native and seven cultivated species, representing seven eudicot families. DNA extracts from the 16 hosts were used for PCR amplification and sequencing of a fragment containing the coat protein (Cp) gene. The complete Cp sequence was used to establish provisional species identification. Results indicated that 13 distinct begomovirus species were represented. Among these, five potentially new begomovirus species were identified, for which we propose the names Anoda golden mosaic virus (AnGMV), Boerhavia yellow spot virus (BoYSV), Papaya golden mosaic virus (PaGMV), Desmodium leaf distortion virus (DeLDV), and Hibiscus variegation virus (HiVV). Five previously described begomoviral species were provisionally identified for the first time in the YPM; these include Euphorbia mosaic virus (EuMV), Melon chlorotic leaf curl virus (MCLCuV), Okra yellow mosaic Mexico virus (OkYMMV), Sida golden mosaic virus (SiGMV), and Tobacco apical stunt virus (TbASV). Additionally, viruses previously reported from this region, Bean golden yellow mosaic virus (BGYMV), Pepper golden mosaic virus (PepGMV), and Tomato mottle virus (ToMoV) were provisionally identified in cultivated hosts. Phylogenetic analysis provisionally placed all isolates from the YPM in a Western Hemisphere begomovirus clade.

  14. A Preliminary Assessment of the Initial Compression Power Requirement in CO2 Pipeline “Carbon Capture and Storage (CCS Technologies”

    Directory of Open Access Journals (Sweden)

    Abdussalam El-Suleiman

    2016-05-01

    Full Text Available CO2 captured from fossil-fueled power generation plants is said to be economically transported via pipelines over long distances. The CO2 must be compressed to pipeline specifications using compressors and pumps that are driven by gas turbine (GT or other prime movers. This paper presents the evaluation of actual work transfer or required prime power by modeling the governing equations of compression using the Peng–Robinson equation of state (PR-EOS. A computer code was developed to carry out the modeling and subsequent simulation of the compression power requirement. The simulation of prime mover power was carried out for different technology (head per stage of the compressor ranging from 10-staged compression to double stage compression. The results show that the current technology of the centrifugal compressor could require as much as 23MW of prime mover power to compress 1.5 million tonnes per year of CO2—a projected equivalent CO2 released from a 530MW combined cycle gas turbine (CCGT power generation plant.

  15. Expansion of the Clavulanic Acid Gene Cluster: Identification and In Vivo Functional Analysis of Three New Genes Required for Biosynthesis of Clavulanic Acid by Streptomyces clavuligerus

    Science.gov (United States)

    Li, Rongfeng; Khaleeli, Nusrat; Townsend, Craig A.

    2000-01-01

    Clavulanic acid is a potent inhibitor of β-lactamase enzymes and is of demonstrated value in the treatment of infections by β-lactam-resistant bacteria. Previously, it was thought that eight contiguous genes within the genome of the producing strain Streptomyces clavuligerus were sufficient for clavulanic acid biosynthesis, because they allowed production of the antibiotic in a heterologous host (K. A. Aidoo, A. S. Paradkar, D. C. Alexander, and S. E. Jensen, p. 219–236, In V. P. Gullo et al., ed., Development in industrial microbiology series, 1993). In contrast, we report the identification of three new genes, orf10 (cyp), orf11 (fd), and orf12, that are required for clavulanic acid biosynthesis as indicated by gene replacement and trans-complementation analysis in S. clavuligerus. These genes are contained within a 3.4-kb DNA fragment located directly downstream of orf9 (cad) in the clavulanic acid cluster. While the orf10 (cyp) and orf11 (fd) proteins show homologies to other known CYP-150 cytochrome P-450 and [3Fe-4S] ferredoxin enzymes and may be responsible for an oxidative reaction late in the pathway, the protein encoded by orf12 shows no significant similarity to any known protein. The results of this study extend the biosynthetic gene cluster for clavulanic acid and attest to the importance of analyzing biosynthetic genes in the context of their natural host. Potential functional roles for these proteins are proposed. PMID:10869089

  16. Preliminary design of high-power wave-guide/transmission system for multimegawatt CW requirements of 100 MeV proton LINAC

    Indian Academy of Sciences (India)

    Purushottam Shrivastava; Y D Wanmode; P R Hannurkar

    2002-11-01

    Development of a 100 MeV CW proton LINAC has been planned at CAT. This LINAC will be needing CW rf power in the frequency ranges of 350 MHz and 700 MHz for its RFQ and DTL/CCDTL/SFDTL structures respectively. The power to the accelerating structures will be produced by either 1 MW CW or 250 kW CW klystrons/inductive output tubes (HOM IOTs). The power needed by respective feed points in the structure is max. 250 kW which will be powered by splitting the power from 1 MW klystron/klystrode into four channels by using a wave-guide system. In case of using 250 kW tubes the power to the structures will be provided directly from each tube. Two types of wave-guide transmission system have been considered, viz, WR 2300 for 350 MHz rf needs and WR 1500 for 700 MHz rf needs. The typical wave-guide system has been designed using the 1 MW CW klystron followed by wave-guide filter, dual directional coupler, high-power circulator, three 3 dB magic TEE power dividers to split the main channel into four equal channels of 250 kW each. Each individual channel has dual directional couplers, flexible wave-guide sections and high power ceramic vacuum window. The circulator and each power divider is terminated into the isolated ports by high power CW loads. Out of the four channels three channels have phase shifters. Present paper describes the technological aspects and design specifications-considerations for these stringent requirements.

  17. Evaluation of effectiveness of a computer system (CAD) in the identification of lung nodules with low-dose MSCT: scanning technique and preliminary results; Valutazione dell'efficacia di un sistema computerizzato (CAD) nell'identificazione dei noduli polmonari con TSMS a basso dosaggio: tecnica di sudio e risultati preliminari

    Energy Technology Data Exchange (ETDEWEB)

    Fraioli, Francesco; Catalano, Carlo; Almberger, Maria; Bertoletti, Linda; Cantisani, Vito; Danti, Massimiliano; Pediconi, Federica; Passariello, Roberto [La Sapienza Univ., Roma (Italy). Dipartimento di Scienze Radiologiche

    2005-02-01

    Purpose: Evaluation of the effectiveness of a computer-aided diagnosis (CAD) in the identification of pulmonary nodules. Materials and methods: Two observers (A1, A2) with different levels of experience independently evaluated 20 chest MSCT studies with and without the aid of a CAD system (LungCheck, R2 Technology, Inc.). The study parameters were as follows: 140 kVs, 40 mAs, collimation 4x1 mm, slice thickness 1.25 mm, reconstruction interval 1.0 mm. The observers analysed the images with and without CAD and evaluated: 1) nodule size (longer axis); 2) number and location of nodules; 3) reading time for each observer. The gold standard was represented by the evaluation of both readers in consensus with the aid of the CAD system. Results: Without CAD support the two readers identified 77 (A1) and 79 (A2) nodules and with CAD 81 (A1) and 82 (A2) nodules. Working in consensus the two observers identified 81 nodules without the aid of CAD and 84 nodules with the aid of CAD. Total number of nodules identified by CAD was 104, 25 of which were false positive and 5 false negative. The average reading time with the aid of CAD decreased by as much as 40% for both the observers. Conclusions: The preliminary results of our study suggest that the CAD technique is an accurate automatic support tool in the identification of pulmonary nodules. It reduces reading time and automatically supplies the size, volume, density and number of nodules, thus being useful both in screening programmes and in the follow-up of cancer patients, in whom comparison of the images is particularly difficult. [Italian] Scopo: Valutazione dell'efficacia di un sistema computerizzato (CAD) nell'identificazione di noduli polmonari. Materiale e metodi: Due osservatori (A1, A2) con differente livello di esperienza hanno valutato 20 esami di TC multistrato del torace con e senza l'ausilio di un sistema CAD (LungCheck, R2 Technology, Inc.). I parametri di studio utilizzati sono stati i seguenti

  18. System Identification

    NARCIS (Netherlands)

    Keesman, K.J.

    2011-01-01

    Summary System Identification Introduction.- Part I: Data-based Identification.- System Response Methods.- Frequency Response Methods.- Correlation Methods.- Part II: Time-invariant Systems Identification.- Static Systems Identification.- Dynamic Systems Identification.- Part III: Time-varying

  19. Agroforestry Systems In Poland A Preliminary Identification

    Science.gov (United States)

    Borek, Robert

    2015-01-01

    This paper seeks to use state-of-the-art knowledge to depict the foundations and prospects for agroforestry systems in Poland to develop, in line with political, legal, historical and environmental conditions pertaining in the country. The main legal provisions concerning the presence of trees in agriculture are presented prior to a first-ever defining of key traditional agroforestry systems in Poland.

  20. Percutaneous renal puncture: requirements and preliminary results

    CERN Document Server

    Leroy, A; Payan, Y; Richard, F; Chartier-Kastler, E; Troccaz, J; Leroy, Antoine; Mozer, Pierre; Payan, Yohan; Chartier-Kastler, Emmanuel; Troccaz, Jocelyne

    2002-01-01

    This paper introduces the principles of computer assisted percutaneous renal puncture, that would provide the surgeon with an accurate pre-operative 3D planning on CT images and, after a rigid registration with space-localized echographic data, would help him to perform the puncture through an intuitive 2D/3D interface. The whole development stage relied on both CT and US images of a healthy subject. We carried out millimetric registrations on real data, then guidance experiments on a kidney phantom showed encouraging results.

  1. Preliminary design data package. Appendix C

    Energy Technology Data Exchange (ETDEWEB)

    1979-07-25

    The design requirements, design philosophy, method and assumptions, and preliminary computer-aided design of the Near-Term Hybrid Vehicle including its electric and heat power units, control equipment, transmission system, body, and overall vehicle characteristics are presented. (LCL)

  2. 红外和紫外可见分光光谱法初步鉴别餐饮废油%Preliminary identification of waste cooking oil by the infrared spectrum and ultraviolet-visible spectrum

    Institute of Scientific and Technical Information of China (English)

    李辰; 梁添旺; 杨伟杰; 许春才; 龚悦

    2014-01-01

    Objective To establish a simple, fast distinguish method for waste cooking oil, biodiesel and qualified cooking oils. Methods Several common cooking oils bought from supermarket, roadside restaurant oil, biodiesel and waste cooking oil (including hogwash oil and frying oil) were compared based on the relative intensity ratio of infrared (IR) spectra with a characteristic absorption peaks 1745 cm-1. Ultraviolet-visible (UV-vis) spectra (230∼800 nm) were successively used to distinguish the difference. Results The relative inten-sity ratio of IR spectra (absorption peaks 1745 cm-1) could be obviously distinguished, especially at 3473 cm-1/1745 cm-1, 3008 cm-1/1745 cm-1 and 1652 cm-1/1745 cm-1, and thus can be used as basis for discrimination. By comparing the starting and end wavelength of UV-vis spectra of the test oils, and whether the spectrum has higher absorbance or obvious absorption peak at 668 nm, the quality of test oil could be recognized. Conclusion The preliminary identification method can be used to quickly identify the qualified oils from waste oils.%目的:建立简便、快速的餐饮废油、生物柴油以及合格食用油的鉴别检测方法。方法选择市售普通食用油、餐馆用油、生物柴油和餐饮废油(包括潲水油和煎炸老油)为研究对象,以1745 cm-1波数处的共有吸收峰为基准,比较各油脂红外光谱特征吸收峰相对强度;在230~800 nm 范围内,比较各油脂的紫外可见吸收曲线,对油脂品质进行比较鉴别。结果比较红外图谱发现,各油脂在3473、3008、1652 cm-1附近对1745 cm-1的吸收峰相对强度差别较大,可以此作为判别依据;通过观察比较各油脂在紫外可见光谱图中的起始和终止吸收波长,以及在668 nm处是否有较高的吸光度或特征吸收峰,可对油脂品质进行鉴别。结论综合红外和紫外可见两种光谱方法的检测结果,本方法可初步地快速鉴别合格食用油与餐饮废油。

  3. Statutory Management Requirements on "Animal Identification and Registration" (Act A7, Act A8: monitoring methods for compliance and related costs in four Italian farms

    Directory of Open Access Journals (Sweden)

    Marisanna Speroni

    2015-11-01

    Full Text Available The European and national laws concerning the identification and registration of livestock are meant to make possible their traceability and facilitate food safety and animal health, especially in case of a disease outbreak; such rules have also become prerequisites that farmers must meet to obtain single farm payments under the common agricultural policy. Failure to comply with these obligations entails the reduction or exclusion from direct payments. Act A7, reports the obligations imposed by the EC Regulation 1760/2000 establishing a system for the identification and registration of bovine animals and regarding the labelling of beef and beef products; Act A8, report the obligations established by the EC Reg. 21/2004 establishing a system for the identification and registration of sheep and goats. The project MO.NA.CO. monitored the application of rules for identification and registration of cattle, sheep and goats and their costs. The system of identification and registration of dairy cows resulted well organized with a good level of coordination between the involved actors in both farms. The activities necessary for compliance with rules of identification and registration of cattle are distributed throughout the year, but vary from day to day. The average total cost for annual obligations amounted to € 533.34 year-1 while the average cost for individual fulfilment in the monitoring period amounted to € 4.10. Even in the case of sheep and goats, the monitoring showed a good cooperation between farms and technicians. However, some difficulties were detected, mainly due to the size of the rumen boluses and the limited effectiveness of the ear tags. The operators suggested using smaller rumen boluses and tattoo instead of ear tags; they also suggest to extend the period within which the animals have to be labelled from the current 6 months to 9 months. The cost of compliance amounted to € 5.27 head-1 for sheep and € 4.90 head-1 for

  4. Hydrothermal Liquefaction Treatment Preliminary Hazard Analysis Report

    Energy Technology Data Exchange (ETDEWEB)

    Lowry, Peter P.; Wagner, Katie A.

    2015-08-31

    A preliminary hazard assessment was completed during February 2015 to evaluate the conceptual design of the modular hydrothermal liquefaction treatment system. The hazard assessment was performed in 2 stages. An initial assessment utilizing Hazard Identification and Preliminary Hazards Analysis (PHA) techniques identified areas with significant or unique hazards (process safety-related hazards) that fall outside of the normal operating envelope of PNNL and warranted additional analysis. The subsequent assessment was based on a qualitative What-If analysis. This analysis was augmented, as necessary, by additional quantitative analysis for scenarios involving a release of hazardous material or energy with the potential for affecting the public.

  5. Preliminary Monthly Climatological Summaries

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Preliminary Local Climatological Data, recorded since 1970 on Weather Burean Form 1030 and then National Weather Service Form F-6. The preliminary climate data pages...

  6. Informatics in radiology: web-based preliminary reporting system for radiology residents with PACS integration.

    Science.gov (United States)

    O'Connell, Timothy; Chang, Debra

    2012-01-01

    While on call, radiology residents review imaging studies and issue preliminary reports to referring clinicians. In the absence of an integrated reporting system at the training sites of the authors' institution, residents were typing and faxing preliminary reports. To partially automate the on-call resident workflow, a Web-based system for resident reporting was developed by using the free open-source xAMP Web application framework and an open-source DICOM (Digital Imaging and Communications in Medicine) software toolkit, with the goals of reducing errors and lowering barriers to education. This reporting system integrates with the picture archiving and communication system to display a worklist of studies. Patient data are automatically entered in the preliminary report to prevent identification errors and simplify the report creation process. When the final report for a resident's on-call study is available, the reporting system queries the report broker for the final report, and then displays the preliminary report side by side with the final report, thus simplifying the review process and encouraging review of all of the resident's reports. The xAMP Web application framework should be considered for development of radiology department informatics projects owing to its zero cost, minimal hardware requirements, ease of programming, and large support community.

  7. Preliminary reference Earth model

    Science.gov (United States)

    Dziewonski, Adam M.; Anderson, Don L.

    1981-06-01

    A large data set consisting of about 1000 normal mode periods, 500 summary travel time observations, 100 normal mode Q values, mass and moment of inertia have been inverted to obtain the radial distribution of elastic properties, Q values and density in the Earth's interior. The data set was supplemented with a special study of 12 years of ISC phase data which yielded an additional 1.75 × 10 6 travel time observations for P and S waves. In order to obtain satisfactory agreement with the entire data set we were required to take into account anelastic dispersion. The introduction of transverse isotropy into the outer 220 km of the mantle was required in order to satisfy the shorter period fundamental toroidal and spheroidal modes. This anisotropy also improved the fit of the larger data set. The horizontal and vertical velocities in the upper mantle differ by 2-4%, both for P and S waves. The mantle below 220 km is not required to be anisotropic. Mantle Rayleigh waves are surprisingly sensitive to compressional velocity in the upper mantle. High S n velocities, low P n velocities and a pronounced low-velocity zone are features of most global inversion models that are suppressed when anisotropy is allowed for in the inversion. The Preliminary Reference Earth Model, PREM, and auxiliary tables showing fits to the data are presented.

  8. THE EFFECTS OF PRELIMINARY RULINGS

    Directory of Open Access Journals (Sweden)

    Iuliana-Mădălina LARION

    2015-07-01

    Full Text Available The study analyses the effects of the preliminary rulings rendered by the Court of Justice for the judicial body that made the reference and for other bodies dealing with similar cases, for the member states, for the European Union’ s institutions and for EU legal order. Starting from the binding effect of the preliminary judgment for national judicial bodies, which requires them to follow the ruling or make a new reference, to the lack of precedent doctrine in EU law, continuing with the possibility to indirectly verify the compatibility of national law of the member states with EU law and ending with the administrative or legislative measures that can or must be taken by the member states, the study intends to highlight the limits, nuances and consequences of the binding effect. It mentions the contribution of the national courts and of the Court of Justice of the European Union to the development of EU law, such as clarifying autonomous notions and it emphasizes the preliminary procedure's attributes of being a form of judicial protection of individual rights, as well as a means to review the legality of acts of EU institutions. The paper is meant to be a useful instrument for practitioners. Therefor, it also deals with the possibility and limits of asking new questions, in order to obtain reconsideration or a refinement of the legal issue and with the problem of judicial control over the interpretation and application of the preliminary ruling by the lower court.

  9. Preliminary Identification of Entomopathogenic Nematodes (Heterorhabditis bacteriophora strain NileKe) and Its Symbiotic Bacteria in Xinjiang%新疆嗜菌异小杆线虫及其共生细菌的初步鉴定研究

    Institute of Scientific and Technical Information of China (English)

    詹发强; 布卡欧尔娜; 侯敏; 杨蓉; 龙宣杞

    2015-01-01

    ;[Result]One kind of entomopathogenic nematodes was isolated from forest soils in Nileke,Xinjiang which could kill Tenebrio molitor larve effectively.It belonged to Heterorhabditis nematodes after a preliminary identification of genus, and named as Heterorhabditis bacteriophora strain Nileke initially.While its symbiotic bacteria were isolated and i_ dentified as Photorhabdus luminescens,but no fluorescence;【Conclusion】The entomopathogenic nematodes and its symbiotic bacteria were the first time successfully separated in Xinjiang and its species were initially i_ dentified,which might offer new options and ways to control pests in the future by entomopathogenic nematodes isolated from Xinjiang and which also established a good foundation to isolate entomapathogenic nematodes in more places in Xinjiang in the near future.

  10. Conjugation in S. pombe: identification of a microtubule-organising centre, a requirement for microtubules and a role for Mad2.

    Science.gov (United States)

    Petersen, J; Heitz, M J; Hagan, I M

    1998-08-27

    During the G1 phase of the cell cycle, cells of the fission yeast Schizosaccharomyces pombe can be induced to mate by nitrogen starvation and the presence of mating pheromones. Polarised growth towards cells of the opposite mating type (P or M) leads to the formation of a projection tip and, upon contact, localised cell wall degradation results in conjugation and cell fusion [1]. Here, we have investigated the role of microtubules in this process. We describe a previously unidentified microtubule-organising centre (MTOC) that forms at projection tips upon cell-to-cell contact, before cells fuse. Treatment of mating cells with the microtubule-destabilising drug thiabendazole (TBZ) showed that microtubule integrity was required for mating at two distinct stages: during projection tip formation and cell fusion. Projection tip formation requires filamentous (F) actin function [2] and microtubules are required for the localisation of F actin to the projection tip. We also identify a role during mating for Mad2--a mitotic checkpoint protein that is required in all eukaryotes to maintain the mitotic state in response to microtubule depolymerisation [3]. S. pombe mad2 mutant cells were compromised in their ability to mate upon removal of TBZ, indicating that in fission yeast, in the absence of microtubules, Mad2 is also required to maintain mating competence.

  11. Writer Identification Using Inexpensive Signal Processing Techniques

    CERN Document Server

    Mokhov, Serguei A; Suen, Ching Y

    2009-01-01

    We propose to use novel and classical audio and text signal-processing and otherwise techniques for "inexpensive" fast writer identification tasks of scanned hand-written documents "visually". The "inexpensive" refers to the efficiency of the identification process in terms of CPU cycles while preserving decent accuracy for preliminary identification. This is a comparative study of multiple algorithm combinations in a pattern recognition pipeline implemented in Java around an open-source Modular Audio Recognition Framework (MARF) that can do a lot more beyond audio. We present our preliminary experimental findings in such an identification task. We simulate "visual" identification by "looking" at the hand-written document as a whole rather than trying to extract fine-grained features out of it prior classification.

  12. Talent Identification

    Directory of Open Access Journals (Sweden)

    Zenita C. Guenther

    2015-12-01

    Full Text Available A considerable part of mental competencies is developed during the early years of life, essentially through sensorial and physical interactions that build up reference points for thinking and acting. However psychometric measures to evaluate babies and infants, up to age 5 or 6, are usually charged with ample margin of random error, generally explained by the diversity of individual differences in developmental rhythms; the same is verified with data collected by direct observation. To search for precocious children at the kindergarten level requires extra caution, because early signs of high ability can be only effects of precocity, that is, a rapid rhythm of neurophysiologic development. A possible exception could be the Intellectual Domain, where behavior expressing signs of General Intelligence (G Domain and linear thinking preference (GV can be observed in association with language development. The present study was designed upon such basis, aiming at validating observable clues and signs of ability and readiness for school learning in Kindergarten children; in the long run it expects to establish the proportion of false positive identification, (children identified for high performance at early ages, who later shows normal ability, and to examine the possibility of effective identification of intellectual giftedness at this age bracket, aiming at developing their potential into academic talent.

  13. Simulated water budgets and ground-water/surface-water interactions in Bushkill and parts of Monocacy Creek watersheds, Northampton County, Pennsylvania--a preliminary study with identification of data needs

    Science.gov (United States)

    Risser, Dennis W.

    2006-01-01

    This report, prepared in cooperation with the Department of Environmental Protection, Office of Mineral Resources Management, provides a preliminary analysis of water budgets and generalized ground-water/surface-water interactions for Bushkill and parts of Monocacy Creek watersheds in Northampton County, Pa., by use of a ground-water flow model. Bushkill Creek watershed was selected for study because it has areas of rapid growth, ground-water withdrawals from a quarry, and proposed stream-channel modifications, all of which have the potential for altering ground-water budgets and the interaction between ground water and streams. Preliminary 2-dimensional, steady-state simulations of ground-water flow by the use of MODFLOW are presented to show the status of work through September 2005 and help guide ongoing data collection in Bushkill Creek watershed. Simulations were conducted for (1) predevelopment conditions, (2) a water table lowered for quarry operations, and (3) anthropogenic changes in hydraulic conductivity of the streambed and aquifer. Preliminary results indicated under predevelopment conditions, the divide between the Bushkill and Monocacy Creek ground-water basins may not have been coincident with the topographic divide and as much as 14 percent of the ground-water discharge to Bushkill Creek may have originated from recharge in the Monocacy Creek watershed. For simulated predevelopment conditions, Schoeneck Creek and parts of Monocacy Creek were dry, but Bushkill Creek was gaining throughout all reaches. Simulated lowering of the deepest quarry sump to an altitude of 147 feet for quarry operations caused ground-water recharge and streamflow leakage to be diverted to the quarry throughout about 14 square miles and caused reaches of Bushkill and Little Bushkill Creeks to change from gaining to losing streams. Lowering the deepest quarry sump to an altitude of 100 feet caused simulated ground-water discharge to the quarry to increase about 4 cubic feet

  14. Preliminary rock mechanics laboratory: Investigation plan

    Energy Technology Data Exchange (ETDEWEB)

    Oschman, K.P.; Hummeldorf, R.G.; Hume, H.R.; Karakouzian, M.; Vakili, J.E.

    1987-01-01

    This document presents the rationale for rock mechanics laboratory testing (including the supporting analysis and numerical modeling) planned for the site characterization of a nuclear waste repository in salt. This plan first identifies what information is required for regulatory and design purposes, and then presents the rationale for the testing that satisfies the required information needs. A preliminary estimate of the minimum sampling requirements for rock laboratory testing during site characterization is also presented. Periodic revision of this document is planned.

  15. 77 FR 69393 - Unique Device Identification System

    Science.gov (United States)

    2012-11-19

    ... HUMAN SERVICES Food and Drug Administration 21 CFR Part 801 RIN 0910-AG31 Unique Device Identification... unique device identification system as required by recent amendments to the Federal Food, Drug, and..., FDA published a proposed rule to establish a unique device identification system, as required by...

  16. Identification of Cis-Acting Elements on Positive-Strand Subgenomic mRNA Required for the Synthesis of Negative-Strand Counterpart in Bovine Coronavirus

    Directory of Open Access Journals (Sweden)

    Po-Yuan Yeh

    2014-07-01

    Full Text Available It has been demonstrated that, in addition to genomic RNA, sgmRNA is able to serve as a template for the synthesis of the negative-strand [(−-strand] complement. However, the cis-acting elements on the positive-strand [(+-strand] sgmRNA required for (−-strand sgmRNA synthesis have not yet been systematically identified. In this study, we employed real-time quantitative reverse transcription polymerase chain reaction to analyze the cis-acting elements on bovine coronavirus (BCoV sgmRNA 7 required for the synthesis of its (−-strand counterpart by deletion mutagenesis. The major findings are as follows. (1 Deletion of the 5'-terminal leader sequence on sgmRNA 7 decreased the synthesis of the (−-strand sgmRNA complement. (2 Deletions of the 3' untranslated region (UTR bulged stem-loop showed no effect on (−-strand sgmRNA synthesis; however, deletion of the 3' UTR pseudoknot decreased the yield of (−-strand sgmRNA. (3 Nucleotides positioned from −15 to −34 of the sgmRNA 7 3'-terminal region are required for efficient (−-strand sgmRNA synthesis. (4 Nucleotide species at the 3'-most position (−1 of sgmRNA 7 is correlated to the efficiency of (−-strand sgmRNA synthesis. These results together suggest, in principle, that the 5'- and 3'-terminal sequences on sgmRNA 7 harbor cis-acting elements are critical for efficient (−-strand sgmRNA synthesis in BCoV.

  17. Identification of Salmonella SPI-2 secretion system components required for SpvB-mediated cytotoxicity in macrophages and virulence in mice.

    Science.gov (United States)

    Browne, Sara H; Hasegawa, Patricia; Okamoto, Sharon; Fierer, Joshua; Guiney, Donald G

    2008-03-01

    The Salmonella SpvB protein possesses ADP-ribosyl transferase activity. SpvB, acting as an intracellular toxin, covalently modifies monomeric actin, leading to loss of F-actin filaments in Salmonella-infected human macrophages. Using defined Salmonella mutants, different functional components of the SPI-2 type three secretion system (TTSS), ssaV, spiC, sseB, sseC, and sseD, were found to be required for SpvB-mediated actin depolymerization in human macrophages. Expression of SpvB protein in Salmonella was not affected by any of the SPI-2 mutants and the effects of these loci were not due to reduced numbers of intracellular bacteria. Interestingly, the major SPI-2 virulence effector, SifA, is not required for SpvB action. Further, caspase-3 activation is an additional marker of cytotoxicity in Salmonella-infected human macrophages. Caspase-3 activity depended on SpvB and SPI-2 TTSS function, but not on SifA. These human macrophage cell culture results were corroborated by virulence studies in mice. Using competitive infection of mice with mixed inocula of single and double mutants, spvBmut1 mutation did not have an effect independent of ssaJ mutation, essential for SPI-2 TTSS function. In contrast, competitive infection studies in mice confirmed that SpvB and SifA have independent virulence effects, as predicted by the macrophage studies.

  18. Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae.

    Science.gov (United States)

    Mayer, M L; Gygi, S P; Aebersold, R; Hieter, P

    2001-05-01

    We have identified and characterized an alternative RFC complex RFC(Ctf18p, Ctf8p, Dcc1p) that is required for sister chromatid cohesion and faithful chromosome transmission. Ctf18p, Ctf8p, and Dcc1p interact physically in a complex with Rfc2p, Rfc3p, Rfc4p, and Rfc5p but not with Rfc1p or Rad24p. Deletion of CTF18, CTF8, or DCC1 singly or in combination (ctf18Deltactf8Deltadcc1Delta) leads to sensitivity to microtubule depolymerizing drugs and a severe sister chromatid cohesion defect. Furthermore, temperature-sensitive mutations in RFC4 result in precocious sister chromatid separation. Our results highlight a novel function of the RFC proteins and support a model in which sister chromatid cohesion is established at the replication fork via a polymerase switching mechanism and a replication-coupled remodeling of chromatin.

  19. Computer-Based Procedures for Field Workers in Nuclear Power Plants: Development of a Model of Procedure Usage and Identification of Requirements

    Energy Technology Data Exchange (ETDEWEB)

    Katya Le Blanc; Johanna Oxstrand

    2012-04-01

    The nuclear industry is constantly trying to find ways to decrease the human error rate, especially the human errors associated with procedure use. As a step toward the goal of improving procedure use performance, researchers, together with the nuclear industry, have been looking at replacing the current paper-based procedures with computer-based procedure systems. The concept of computer-based procedures is not new by any means; however most research has focused on procedures used in the main control room. Procedures reviewed in these efforts are mainly emergency operating procedures and normal operating procedures. Based on lessons learned for these previous efforts we are now exploring a more unknown application for computer based procedures - field procedures, i.e. procedures used by nuclear equipment operators and maintenance technicians. The Idaho National Laboratory and participants from the U.S. commercial nuclear industry are collaborating in an applied research effort with the objective of developing requirements and specifications for a computer-based procedure system to be used by field workers. The goal is to identify the types of human errors that can be mitigated by using computer-based procedures and how to best design the computer-based procedures to do so. This paper describes the development of a Model of Procedure Use and the qualitative study on which the model is based. The study was conducted in collaboration with four nuclear utilities and five research institutes. During the qualitative study and the model development requirements and for computer-based procedures were identified.

  20. Preliminary Opto-Mechanical Design for the X2000 Transceiver

    Science.gov (United States)

    Hemmati, H.; Page, N. A.

    2000-01-01

    Preliminary optical design and mechanical conceptual design for a 30 cm aperture transceiver are described. A common aperture is used for both transmit and receive. Special attention was given to off-axis and scattered light rejection and isolation of the receive channel from the transmit channel. Requirements, details of the design and preliminary performance analysis of the transceiver are provided.

  1. Preliminary analysis of patent trends for magnetic fusion technology

    Energy Technology Data Exchange (ETDEWEB)

    Levine, L.O.; Ashton, W.B.; Campbell, R.S.

    1984-02-01

    This study presents a preliminary analysis of development trends in magnetic fusion technology based on data from US patents. The research is limited to identification and description of general patent activity and ownership characteristics for 373 patents. The results suggest that more detailed studies of fusion patents could provide useful R and D planning information.

  2. Infrared and visible combat identification marking materials

    Science.gov (United States)

    O'Keefe, Eoin; Shohet, Adam; Swan, Martin

    2007-04-01

    Historically, it is believed that fratricide accounts for up to 15% of friendly casualties during operations and a UK MoD report identifies that almost half of all such casualties occur in situations involving ground units only. Such risks can be mitigated, to an extent, via operational awareness and effective communications. However, recent conflicts have involved a much more dynamic, complex and technically sophisticated battlefield than previously experienced. For example, Operation Telic (Desert Storm) involved almost one million combatants and ten thousand armoured vehicles in the coalition force, advancing across an extensive battlefront at high speed during daylight and at night, making effective use of a range of electro-optic sensors. The accelerated tempo of battle means that front lines can undergo rapid, punctuated advances that can leave individual combat units with a much degraded situational awareness, particularly of where they are in relation to other 'friendly' combatants. Consequently, there is a need for a robust, low cost, low weight, compact, unpowered, interoperable, Combat Identification technique for use with popular electro-optic sensors which can be deployed, and is effective, at the individual combat unit level. In this paper we discuss ground-to-ground combat identification materials that meet these requirements, all of which are based on the air-to-ground Mirage TM vehicle marking material. We show some preliminary ground-to-ground data collected from the new variant Mirage TM material in recent experimental trials conducted during the day, evening and at night.

  3. Greater-than-Class C low-level radioactive waste shipping package/container identification and requirements study. National Low-Level Waste Management Program

    Energy Technology Data Exchange (ETDEWEB)

    Tyacke, M.

    1993-08-01

    This report identifies a variety of shipping packages (also referred to as casks) and waste containers currently available or being developed that could be used for greater-than-Class C (GTCC) low-level waste (LLW). Since GTCC LLW varies greatly in size, shape, and activity levels, the casks and waste containers that could be used range in size from small, to accommodate a single sealed radiation source, to very large-capacity casks/canisters used to transport or dry-store highly radioactive spent fuel. In some cases, the waste containers may serve directly as shipping packages, while in other cases, the containers would need to be placed in a transport cask. For the purpose of this report, it is assumed that the generator is responsible for transporting the waste to a Department of Energy (DOE) storage, treatment, or disposal facility. Unless DOE establishes specific acceptance criteria, the receiving facility would need the capability to accept any of the casks and waste containers identified in this report. In identifying potential casks and waste containers, no consideration was given to their adequacy relative to handling, storage, treatment, and disposal. Those considerations must be addressed separately as the capabilities of the receiving facility and the handling requirements and operations are better understood.

  4. Conceptual waste package interim product specifications and data requirements for disposal of borosilicate glass defense high-level waste forms in salt geologic repositories

    Energy Technology Data Exchange (ETDEWEB)

    1983-06-01

    The conceptual waste package interim product specifications and data requirements presented are applicable specifically to the normal borosilicate glass product of the Defense Waste Processing Facility (DWPF). They provide preliminary numerical values for the defense high-level waste form parameters and properties identified in the waste form performance specification for geologic isolation in salt repositories. Subject areas treated include containment and isolation, operational period safety, criticality control, waste form/production canister identification, and waste package performance testing requirements. This document was generated for use in the development of conceptual waste package designs in salt. It will be revised as additional data, analyses, and regulatory requirements become available.

  5. Review of current severe accident management approaches in Europe and identification of related modelling requirements for the computer code ASTEC V2.1

    Energy Technology Data Exchange (ETDEWEB)

    Hermsmeyer, S. [European Commission JRC, Petten (Netherlands). Inst. for Energy and Transport; Herranz, L.E.; Iglesias, R. [CIEMAT, Madrid (Spain); and others

    2015-07-15

    The severe accident at the Fukushima-Daiichi nuclear power plant (NPP) has led to a worldwide review of nuclear safety approaches and is bringing a refocussing of R and D in the field. To support these efforts several new Euratom FP7 projects have been launched. The CESAM project focuses on the improvement of the ASTEC computer code. ASTEC is jointly developed by IRSN and GRS and is considered as the European reference code for Severe Accident Analyses since it capitalizes knowledge from the extensive Euro-pean R and D in the field. The project aims at the code's enhancement and extension for use in Severe Accident Management (SAM) analysis of the NPPs of Generation II-III presently under operation or foreseen in the near future in Europe, spent fuel pools included. The work reported here is concerned with the importance, for the further development of the code, of SAM strategies to be simulated. To this end, SAM strategies applied in the EU have been compiled. This compilation is mainly based on the public information made available in the frame of the EU ''stress tests'' for NPPs and has been complemented by information pro-vided by the different CESAM partners. The context of SAM is explained and the strategies are presented. The modelling capabilities for the simulation of these strategies in the current production version 2.0 of ASTEC are discussed. Furthermore, the requirements for the next version of ASTEC V2.1 that is supported in the CESAM project are highlighted. They are a necessary complement to the list of code improvements that is drawn from consolidating new fields of application, like SFP and BWR model enhancements, and from new experimental results on severe accident phenomena.

  6. Mutational analysis of the cytoplasmic tail of the human transferrin receptor. Identification of a sub-domain that is required for rapid endocytosis.

    Science.gov (United States)

    Gironès, N; Alverez, E; Seth, A; Lin, I M; Latour, D A; Davis, R J

    1991-10-05

    It has been reported that the sequence Tyr20-X-Arg-Phe23 present within the cytoplasmic tail of the transferrin receptor may represent a tyrosine internalization signal (Collawn, J.F., Stangel, M., Kuhn, L.A., Esekogwu, V., Jing, S., Trowbridge, I.S., and Tainer, J. A. (1990) Cell 63, 1061-1072). However, as Tyr20 is not conserved between species (Alvarez, E., Gironès, N., and Davis, R. J. (1990) Biochem. J. 267, 31-35), the functional role of the putative tyrosine internalization signal is not clear. To address this question, we constructed a series of 32 deletions and point mutations within the cytoplasmic tail of the human transferrin receptor. The effect of these mutations on the apparent first order rate constant for receptor endocytosis was examined. It was found that the region of the cytoplasmic tail that is proximal to the transmembrane domain (residues 28-58) is dispensable for rapid endocytosis. In contrast, the distal region of the cytoplasmic tail (residues 1-27) was found to be both necessary and sufficient for the rapid internalization of the transferrin receptor. The region identified includes Tyr20-X-Arg-Phe23, but is significantly larger than this tetrapeptide. It is therefore likely that structural information in addition to the proposed tyrosine internalization signal is required for endocytosis. To test this hypothesis, we investigated whether a heterologous tyrosine internalization signal (from the low density lipoprotein receptor) could function to cause the rapid endocytosis of the transferrin receptor. It was observed that this heterologous tyrosine internalization signal did not allow rapid endocytosis. We conclude that the putative tyrosine internalization signal (Tyr20-Thr-Arg-Phe23) is not sufficient to determine rapid endocytosis of the transferrin receptor. The data reported here indicate that the transferrin receptor internalization signal is formed by a larger cytoplasmic tail structure located at the amino terminus of the receptor.

  7. Preliminary Results on Charmed Meson Spectroscopy

    CERN Document Server

    Sarwar, S; Paolone, V S; Reyes, M; Anjos, J C; Yager, P M; Bediaga, I; Göbel, C; Magnin, J; De Miranda, J M; Pepe, I M; Dos Reis, A C; Simão, F R A; Carrillo, S; Casimiro, E; Méndez, H; Sánchez-Hernández, A; Uribe, C; Vásquez, F; Cinquini, L; Cumalat, J P; Ramírez, J E; O'Reilly, B; Vaandering, E W; Butler, J N; Cheung, H W K; Gaines, I; Garbincius, P H; Garren, L A; Gottschalk, E E; Gourlay, S A; Kasper, P H; Kreymer, A E; Kutschke, R; Bianco, S; Fabbri, Franco Luigi; Sarwar, S; Cawlfield, C; Kim, D Y; Park, K S; Rahimi, A; Gardner, R; Chung, Y S; Kang, J S; Ko, B R; Kwak, J W; Lee, K B; Myung, S S; Alimonti, G; Boschini, M; Brambilla, D; Caccianiga, B; Calandrino, A; D'Angelo, P; Di Corato, M; Dini, P; Giammarchi, M G; Inzani, P; Leveraro, F; Malvezzi, S; Menasce, D; Mezzadri, M; Milazzo, L; Moroni, L; Pedrini, D; Prelz, F; Rovere, M; Sala, A; Sala, S; Arena, V; Boca, G; Bonomi, G; Gianini, G; Liguori, G; Merlo, M; Pantea, D; Ratti, S P; Riccardi, C; Torre, P; Viola, L; Vitulo, P; Hernández, Pilar; López, A M; Méndez, L; Mirles, M A; Montiel, E; Olaya, D; Quinones, J; Rivera, C; Zhang-Mayaguez, Y; Copty, N K; Purohit, M; Cho, K; Handler, T; Engh, D; Johns, W E; Hosack, M; Nehring, M S; Sales, M; Sheldon, P D; Stenson, K; Webster, M S; Sheaff, M; Kwon, Y; Sarwar, Shahzad

    2001-01-01

    We report the preliminary measurement by the FOCUS Collaboration (E831 at Fermilab) of masses and widths of the L=1 charm mesons $D_2^{*0}$ and $D_2^{*+}$. The fit of the invariant mass distribution requires an additional term to account for a broad structure over background.

  8. 2016 TRI Preliminary Dataset

    Science.gov (United States)

    The TRI preliminary dataset includes the most current TRI data available and reflects toxic chemical releases and pollution prevention activities that occurred at TRI facilities during the 2016 calendar year.

  9. P and e identification capabilities CAPRICE

    Energy Technology Data Exchange (ETDEWEB)

    Basini, G. [INFN, Laboratori Nazionali di Frascati, Rome (Italy); Codino, A.; Grimani, C. [Perugia Univ. (Italy)]|[INFN, Perugia (Italy); De Pascale, M.P. [Rome Univ. `Tor Vergata` (Italy)]|[INFN, Rome (Italy); Hof, M. [Siegen Univ. (Germany). Fachbereich Physik; Golden, R.L. [New Mexico State Univ., Las Cruces, NM (United States). Particle Astrophysics Lab.; Brancaccio, F.; Bocciolini, M. [Florence Univ. (Italy)]|[INFN, Florence (Italy); Barbiellini, G.; Boezio, M. [Trieste Univ. (Italy)]|[INFN, Trieste (Italy)

    1995-09-01

    The cosmic antiparticle ring imaging Cherenkov experiment (CAPRICE) flew on a stratospheric balloon 8-9 August 1994 over northern Canada and collected data for more than 21 hours with less than 5 g/cm{sup 2} of residual atmosphere. The instrument includes a solid radiator RICH detector and an electromagnetic calorimeter for particle identification in the magnetic spectrometer. Preliminary antiproton and positron identification capabilities are presented.

  10. Identification and Characterization of a Novel Issatchenkia orientalis GPI-Anchored Protein, IoGas1, Required for Resistance to Low pH and Salt Stress.

    Science.gov (United States)

    Matsushika, Akinori; Negi, Kanako; Suzuki, Toshihiro; Goshima, Tetsuya; Hoshino, Tamotsu

    2016-01-01

    The use of yeasts tolerant to acid (low pH) and salt stress is of industrial importance for several bioproduction processes. To identify new candidate genes having potential roles in low-pH tolerance, we screened an expression genomic DNA library of a multiple-stress-tolerant yeast, Issatchenkia orientalis (Pichia kudriavzevii), for clones that allowed Saccharomyces cerevisiae cells to grow under highly acidic conditions (pH 2.0). A genomic DNA clone containing two putative open reading frames was obtained, of which the putative protein-coding gene comprising 1629 bp was retransformed into the host. This transformant grew significantly at pH 2.0, and at pH 2.5 in the presence of 7.5% Na2SO4. The predicted amino acid sequence of this new gene, named I. orientalis GAS1 (IoGAS1), was 60% identical to the S. cerevisiae Gas1 protein, a glycosylphosphatidylinositol-anchored protein essential for maintaining cell wall integrity, and 58-59% identical to Candida albicans Phr1 and Phr2, pH-responsive proteins implicated in cell wall assembly and virulence. Northern hybridization analyses indicated that, as for the C. albicans homologs, IoGAS1 expression was pH-dependent, with expression increasing with decreasing pH (from 4.0 to 2.0) of the medium. These results suggest that IoGAS1 represents a novel pH-regulated system required for the adaptation of I. orientalis to environments of diverse pH. Heterologous expression of IoGAS1 complemented the growth and morphological defects of a S. cerevisiae gas1Δ mutant, demonstrating that IoGAS1 and the corresponding S. cerevisiae gene play similar roles in cell wall biosynthesis. Site-directed mutagenesis experiments revealed that two conserved glutamate residues (E161 and E262) in the IoGas1 protein play a crucial role in yeast morphogenesis and tolerance to low pH and salt stress. Furthermore, overexpression of IoGAS1 in S. cerevisiae remarkably improved the ethanol fermentation ability at pH 2.5, and at pH 2.0 in the presence of

  11. Identification of a Residue (Glu60) in TRAP Required for Inducing Efficient Transcription Termination at the trp Attenuator Independent of Binding Tryptophan and RNA.

    Science.gov (United States)

    McAdams, Natalie M; Patterson, Andrea; Gollnick, Paul

    2017-03-15

    Transcription of the tryptophan (trp) operon in Bacillus subtilis is regulated by an attenuation mechanism. Attenuation is controlled by the trpRNA-binding attenuation protein (TRAP). TRAP binds to a site in the 5' leader region of the nascent trp transcript in response to the presence of excess intracellular tryptophan. This binding induces transcription termination upstream of the structural genes of the operon. In prior attenuation models, the role of TRAP was only to alter the secondary structure of the leader region RNA so as to promote formation of the trp attenuator, which was presumed to function as an intrinsic terminator. However, formation of the attenuator alone has been shown to be insufficient to induce efficient termination, indicating that TRAP plays an additional role in this process. To further examine the function of TRAP, we performed a genetic selection for mutant TRAPs that bind tryptophan and RNA but show diminished termination at the trp attenuator. Five such TRAP mutants were obtained. Four of these have substitutions at Glu60, three of which are Lys (E60K) substitutions and the fourth of which is a Val (E60V) substitution. The fifth mutant obtained contains a substitution at Ile63, which is on the same β-strand of TRAP as Glu60. Purified E60K TRAP binds tryptophan and RNA with properties similar to those of the wild type but is defective at inducing termination at the trp attenuator in vitroIMPORTANCE Prior models for attenuation control of the B. subtilis trp operon suggested that the only role for TRAP is to bind to the leader region RNA and alter its folding to induce formation of an intrinsic terminator. However, several recent studies suggested that TRAP plays an additional role in the termination mechanism. We hypothesized that this function could involve residues in TRAP other than those required to bind tryptophan and RNA. Here we obtained TRAP mutants with alterations at Glu60 that are deficient at inducing termination in the

  12. Identification and Characterization of a Novel Issatchenkia orientalis GPI-Anchored Protein, IoGas1, Required for Resistance to Low pH and Salt Stress

    Science.gov (United States)

    Matsushika, Akinori; Negi, Kanako; Suzuki, Toshihiro; Goshima, Tetsuya; Hoshino, Tamotsu

    2016-01-01

    The use of yeasts tolerant to acid (low pH) and salt stress is of industrial importance for several bioproduction processes. To identify new candidate genes having potential roles in low-pH tolerance, we screened an expression genomic DNA library of a multiple-stress-tolerant yeast, Issatchenkia orientalis (Pichia kudriavzevii), for clones that allowed Saccharomyces cerevisiae cells to grow under highly acidic conditions (pH 2.0). A genomic DNA clone containing two putative open reading frames was obtained, of which the putative protein-coding gene comprising 1629 bp was retransformed into the host. This transformant grew significantly at pH 2.0, and at pH 2.5 in the presence of 7.5% Na2SO4. The predicted amino acid sequence of this new gene, named I. orientalis GAS1 (IoGAS1), was 60% identical to the S. cerevisiae Gas1 protein, a glycosylphosphatidylinositol-anchored protein essential for maintaining cell wall integrity, and 58–59% identical to Candida albicans Phr1 and Phr2, pH-responsive proteins implicated in cell wall assembly and virulence. Northern hybridization analyses indicated that, as for the C. albicans homologs, IoGAS1 expression was pH-dependent, with expression increasing with decreasing pH (from 4.0 to 2.0) of the medium. These results suggest that IoGAS1 represents a novel pH-regulated system required for the adaptation of I. orientalis to environments of diverse pH. Heterologous expression of IoGAS1 complemented the growth and morphological defects of a S. cerevisiae gas1Δ mutant, demonstrating that IoGAS1 and the corresponding S. cerevisiae gene play similar roles in cell wall biosynthesis. Site-directed mutagenesis experiments revealed that two conserved glutamate residues (E161 and E262) in the IoGas1 protein play a crucial role in yeast morphogenesis and tolerance to low pH and salt stress. Furthermore, overexpression of IoGAS1 in S. cerevisiae remarkably improved the ethanol fermentation ability at pH 2.5, and at pH 2.0 in the presence of

  13. Identification of Amino Acid Residues in Fibroblast Growth Factor 14 (FGF14) Required for Structure-Function Interactions with Voltage-gated Sodium Channel Nav1.6.

    Science.gov (United States)

    Ali, Syed R; Singh, Aditya K; Laezza, Fernanda

    2016-05-20

    The voltage-gated Na(+) (Nav) channel provides the basis for electrical excitability in the brain. This channel is regulated by a number of accessory proteins including fibroblast growth factor 14 (FGF14), a member of the intracellular FGF family. In addition to forming homodimers, FGF14 binds directly to the Nav1.6 channel C-tail, regulating channel gating and expression, properties that are required for intrinsic excitability in neurons. Seeking amino acid residues with unique roles at the protein-protein interaction interface (PPI) of FGF14·Nav1.6, we engineered model-guided mutations of FGF14 and validated their impact on the FGF14·Nav1.6 complex and the FGF14:FGF14 dimer formation using a luciferase assay. Divergence was found in the β-9 sheet of FGF14 where an alanine (Ala) mutation of Val-160 impaired binding to Nav1.6 but had no effect on FGF14:FGF14 dimer formation. Additional analysis revealed also a key role of residues Lys-74/Ile-76 at the N-terminal of FGF14 in the FGF14·Nav1.6 complex and FGF14:FGF14 dimer formation. Using whole-cell patch clamp electrophysiology, we demonstrated that either the FGF14(V160A) or the FGF14(K74A/I76A) mutation was sufficient to abolish the FGF14-dependent regulation of peak transient Na(+) currents and the voltage-dependent activation and steady-state inactivation of Nav1.6; but only V160A with a concomitant alanine mutation at Tyr-158 could impede FGF14-dependent modulation of the channel fast inactivation. Intrinsic fluorescence spectroscopy of purified proteins confirmed a stronger binding reduction of FGF14(V160A) to the Nav1.6 C-tail compared with FGF14(K74A/I76A) Altogether these studies indicate that the β-9 sheet and the N terminus of FGF14 are well positioned targets for drug development of PPI-based allosteric modulators of Nav channels.

  14. Screening and Preliminary Identification for a Strain of Nitrogen -Fixing Bacterium Producing PHB%一株产 PHB 固氮菌的筛选和初步鉴定

    Institute of Scientific and Technical Information of China (English)

    李贵正; 涂国全; 刘纪臣; 李新柱

    2015-01-01

    Eight nitrogen -fixing bacteria were isolated from the activated sludge from sewage farm of Kingenta by enriching culture and isolating in Ashby medium.Three strains producing PHB were obtained by Suda black staining.Strain N1 was regarded as the best one by the PHB diameter,content of PHB account for cell dry weight and the colony growth rate.Strain N1 was preliminary identified as Azotobacter chroococcum.%经 Ashby 培养基富集培养及平板分离,从金正大污水处理厂的活性污泥中分离到8株自生固氮菌。经苏丹黑染色初筛获得3株产 PHB 菌株。以菌株产生的 PHB 颗粒大小、PHB 占细胞干重的含量及菌落生长速度为指标,获得最佳菌株 N1。通过《伯杰氏细菌学鉴定手册》第九版初步鉴定此菌株为圆褐固氮菌(Azotobacter chroococcum)。

  15. Isolation ,Identification and the Preliminary Establishment of the Multiplex-PCR of Pathogens from Soft-shelled Turtle%中华鳖病原菌菌种的分离鉴定及多重PCR的初步建立

    Institute of Scientific and Technical Information of China (English)

    燕晶; 刘文青; 高珊珊; 赵娜; 张亚宁; 赵宝华

    2013-01-01

    We identified the suspected pathogenic bacteria isolated from heart,liver and intestinal tract of soft-shelled turtle through conventional physiological and biochemical identification techniques. Multi plex PCR,which used primers designed according to conserved sequences in bacterial 16s rDNA,was also used for pathogen identification by gene amplification and sequence alignment,and the reaction conditions of multiplex PCR were optimized. By sequencing,Edwardsiella tarda (Et) f Aeromonas hydrophila (Ah) , Aeromonas veronii(Av) ,Citrobacter freundii(Cf) were identified. The high sensitivity and good specifici ty, stability and repeatability proved that the multiplex PCR was practical to identify pathogens from soft-shelled turtles.%对患病中华鳖心脏、肝脏、肠道中分离得到的疑似致病菌株进行常规生理生化鉴定,同时采用分子生物学鉴定方法,设计细菌16s rDNA保守区通用引物,进行基因扩增及序列比对,建立并优化多重PCR反应条件.分离鉴定出4种病原菌:迟钝爱德华氏菌(Edwardsiella tarda,Et)、嗜水气单胞菌(Aeromonash ydrophila,Ah)、维氏气单胞菌(Aeromonas veronii,Av)、弗氏柠檬酸杆菌(Citrobacter freundii,Cf).多重PCR检测体系敏感性与特异性良好,证明多重PCR是一种能快速准确鉴定多种中华鳖致病菌的方法,且操作简单、灵敏度高、稳定性和重复性好.

  16. 一株银杏内生裂褶菌的鉴定及其产黄酮的初步研究%Preliminary Study on Identification of and Flavonoid Production from an Endophytic Fugus from Gink go biloba L.

    Institute of Scientific and Technical Information of China (English)

    韩晓丽; 康冀川; 段宝玲; 冯强

    2011-01-01

    为了解银杏内生真茵产黄酮情况,对从贵州大学校园银杏树叶中分离到的一株内生真茵GZUY201进行鉴定及产黄酮的研究.菌株鉴定利用形态学和分子生物学相结合的方法,并通过HPLC法测定其产黄酮的含量.鉴定菌株GZUY201为裂褶菌(Schizoph yllum commune Fr.),菌株发酵液和菌丝体产黄酮苷元槲皮素量可达40.8 mg·L-1和101.7 mg·g-1.银杏内生裂褶茵可产黄酮苷元槲皮素,可作为生产银杏黄酮的候选菌株.%In order to understand Ginkgo biloba endophytic fungi in brass,the research aimed to study on the identification of an endophytic fungus strain GZUY201. which was isolated from the leaves of Ginkgo biloba L. , and the productivity of flavonoids from the fungus. The identification based on morphological and molecular methods, the flavoniods content were detected by means of HPLC. The endophytic fungus GZUY201 was identified as Schizophyllum commune Fr. and it can produce quercetin which is a kind of flavonoid glycoside up to 40. 8 mg · L-1 and 101. 7 μg · g-1. The endophytic fungus can produce quercetin and can be used as an alternative strain for flavonoids production.

  17. 37 CFR 1.488 - Determination of unity of invention before the International Preliminary Examining Authority.

    Science.gov (United States)

    2010-07-01

    ... invention before the International Preliminary Examining Authority. 1.488 Section 1.488 Patents, Trademarks... Determination of unity of invention before the International Preliminary Examining Authority. (a) Before... requirement of unity of invention as set forth in § 1.475. (b) If the International Preliminary Examining...

  18. Crowd-Centric Requirements Engineering

    NARCIS (Netherlands)

    Snijders, Remco; Dalpiaz, Fabiano; Hosseini, Mahmood; Shahri, Alimohammad; Ali, Raian

    2014-01-01

    Requirements engineering is a preliminary and crucial phase for the correctness and quality of software systems. Despite the agreement on the positive correlation between user involvement in requirements engineering and software success, current development methods employ a too narrow concept of tha

  19. 毒蕈与易混淆可食蕈菌识别研究初探%Preliminary study on the identification of poisonous mushrooms and confusable edible mushrooms

    Institute of Scientific and Technical Information of China (English)

    谢立璟; 唐丽萍

    2013-01-01

    Objective To explore whether it would be possible to distinguish the poisonous mushrooms and the confusable edible mushrooms by visualized method and provide the basis for prevention and treatment of mushroom poisoning.Methods The types of dangerous poisonous mushrooms were confirmed by literature and experts' opinions.The macroscopical morphological features were compared between the common poisonous mushrooms and the edible mushrooms.The specimens of mushroom were field-collected and the macroscopical and microcosmic features were identified by traditional morphological method.The visualized identification methods of poisonous mushrooms reported in literature,books and periodicals,and circulated among the people were tested and verified with examples.Results Fifteen kinds of common and dangerous poisonous mushrooms were selected preliminarily.The macroscopical morphological features were compared between the common poisonous mushrooms and the confusable edible mushrooms according to the professional books,literature and the experience.The results of the abovementioned comparison proved that it was impossible to distinguish the poisonous mushrooms and the confusable edible mushrooms according to macroscopical morphological features only.The traditional morphological features (including both macroscopical and microcosmic features) of 12 kinds of fieldcollected mushroom species were identified.The results showed that the identification of mushroom's type must be completed by the taxonomic professionals according to the macroscopical and microcosmic features.The results of verification showed that none of the 6 kinds of visualized methods which were widely circulated could be used as general identification method of poisonous mushrooms.Conclusions There is no simple and general visualized identification method for poisonous mushrooms at present.The stress of control poisonous mushrooms poisoning should be put on the study of identifying technique and the correction

  20. Crystallization and identification of the glycosylated moieties of two isoforms of the main allergen Hev b 2 and preliminary X-ray analysis of two polymorphs of isoform II

    Energy Technology Data Exchange (ETDEWEB)

    Fuentes-Silva, D. [Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Cuidad Universitaria, Coyoacán, México, DF 04510 (Mexico); Mendoza-Hernández, G. [Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Cuidad Universitaria, Coyoacán, México, DF 04510 (Mexico); Stojanoff, V. [Brookhaven National Laboratory, National Synchrotron Light Source, Upton, NY (United States); Palomares, L. A. [Instituto de Biotecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Cuidad Universitaria, Coyoacán, México, DF 04510 (Mexico); Zenteno, E. [Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Cuidad Universitaria, Coyoacán, México, DF 04510 (Mexico); Torres-Larios, A. [Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Cuidad Universitaria, Coyoacán, México, DF 04510 (Mexico); Rodríguez-Romero, A., E-mail: adela@servidor.unam.mx [Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Cuidad Universitaria, Coyoacán, México, DF 04510 (Mexico)

    2007-09-01

    Crystallization of important glycoenzymes involved in IgE-mediated latex allergy. Latex from Hevea brasiliensis contains several allergenic proteins that are involved in type I allergy. One of them is Hev b 2, which is a β-1,3-glucanase enzyme that exists in different isoforms with variable glycosylation content. Two glucanase isoforms were isolated from trees of the GV-42 clone by gel filtration, affinity and ion-exchange chromatography. Isoform I had a carbohydrate content of about 20%, with N-linked N-acetyl-glucosamine, N-acetyl-galactosamine, fucose and galactose residues as the main sugars, while isoform II showed 6% carbohydrate content constisting of N-acetyl-glucosamine, fucose, mannose and xylose. Both isoforms were crystallized by the hanging-drop vapour-diffusion method. Isoform I crystals were grown using 0.2 M trisodium citrate dihydrate, 0.1 M Na HEPES pH 7.5 and 20%(v/v) 2-propanol, but these crystals were not appropriate for data collection. Isoform II crystals were obtained under two conditions and X-ray diffraction data were collected from both. In the first condition (0.2 M trisodium citrate, 0.1 M sodium cacodylate pH 6.5, 30% 2-propanol), crystals belonging to the tetragonal space group P4{sub 1} with unit-cell parameters a = b = 150.17, c = 77.41 Å were obtained. In the second condition [0.2 M ammonium acetate, 0.1 M trisodium citrate dihydrate pH 5.6, 30%(w/v) polyethylene glycol 4000] the isoform II crystals belonged to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 85.08, b = 89.67, c = 101.80 Å, β = 113.6°. Preliminary analysis suggests that there are four molecules of isoform II in both asymmetric units.

  1. Crystallization and Identification of the Glycosylated Moieties of Two Isoforms of the Main Allergen Hev b 2 and Preliminary X-ray Analysis of Two Polymorphs of Isoform ll

    Energy Technology Data Exchange (ETDEWEB)

    Fuentes-Silva,D.; Mendoza-Hernandez, G.; Stojanoff, V.; Palomares, L.; Zenteno, E.; Torres-Larios, A.; Rodriguez-Romero, A.

    2007-01-01

    Latex from Hevea brasiliensis contains several allergenic proteins that are involved in type I allergy. One of them is Hev b 2, which is a {beta}-1,3-glucanase enzyme that exists in different isoforms with variable glycosylation content. Two glucanase isoforms were isolated from trees of the GV-42 clone by gel filtration, affinity and ion-exchange chromatography. Isoform I had a carbohydrate content of about 20%, with N-linked N-acetyl-glucosamine, N-acetyl-galactosamine, fucose and galactose residues as the main sugars, while isoform II showed 6% carbohydrate content consisting of N-acetyl-glucosamine, fucose, mannose and xylose. Both isoforms were crystallized by the hanging-drop vapor-diffusion method. Isoform I crystals were grown using 0.2 M trisodium citrate dihydrate, 0.1 M Na HEPES pH 7.5 and 20%(v/v) 2-propanol, but these crystals were not appropriate for data collection. Isoform II crystals were obtained under two conditions and X-ray diffraction data were collected from both. In the first condition (0.2 M trisodium citrate, 0.1 M sodium cacodylate pH 6.5, 30% 2-propanol), crystals belonging to the tetragonal space group P4{sub 1} with unit-cell parameters a = b = 150.17, c = 77.41 {angstrom} were obtained. In the second condition [0.2 M ammonium acetate, 0.1 M trisodium citrate dihydrate pH 5.6, 30%(w/v) polyethylene glycol 4000] the isoform II crystals belonged to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 85.08, b = 89.67, c = 101.80 {angstrom}, {beta}= 113.6{sup o}. Preliminary analysis suggests that there are four molecules of isoform II in both asymmetric units.

  2. 铬(Ⅵ)土著还原菌筛选及初步鉴定实验研究%Experimental Study on Indigenous Chromium (Ⅵ)-Reducing Bacteria Screen and Preliminary Identification

    Institute of Scientific and Technical Information of China (English)

    赵光辉; 常文越; 李雄勇; 刘智; 王馨

    2013-01-01

    Chromium (Ⅵ) indigenous reducing bacteria were screened using solid culture in which potassium dichromate was added as test material.16 chromium (Ⅵ) indigenous reducing bacteria stains were screened out and Z2 (35.2%),Z3 (45.2%),Z4 (38.6%),X8 (30.4%),X10 (29.4%) for their relatively high chromium (Ⅵ) reduction rate were picked out as chromium (Ⅵ)reduction advantages strains.Slide culture method,Gram staining,flagella staining,spore staining were used for reducing bacteria primary identification.Primary identification results are as follows:indigenous fungus Z2 is classified as Aspergillus niger; Z3 and Z4 are Fusarium sp.,indigenous bacteria X8,X10 are Bacillus sp.Experimental methods and results in this paper can provide technical support for popularization and application of microbial technology on chromium (Ⅵ) pollution control.%以重铬酸钾为供试物,用固体平板法和还原实验对铬(Ⅵ)土著还原菌进行筛选,用载片培养法、革兰氏染色法、鞭毛染色法、芽孢染色法对还原菌做初步的鉴定,共筛选出16株铬(Ⅵ)土著还原菌,并挑选出还原率相对较高的Z2(35.2%)、Z3(45.2%)、Z4(38.6%)、X8(30.4%)、X10(29.4%)作为铬(Ⅵ)的优势还原菌株.经初步鉴定,土著真菌Z2为黑曲霉(Aspergillus niger),Z3、Z4为镰刀菌属(Fusarium sp.),土著细菌X8、X10为芽孢杆菌(Bacillus sp.).实验的方法和成果将为铬(Ⅵ)污染土壤微生物治理技术的推广应用提供技术支持.

  3. 抗犬瘟热病毒荧光标记单抗的制备和初步鉴定%Preparation and Preliminary Identification of Fluorescein Labeled Monoclonal Antibody against Canine Distemper Virus

    Institute of Scientific and Technical Information of China (English)

    苏建青; 褚秀玲; 杨松涛; 夏咸柱; 岳妙姝

    2009-01-01

    [Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper (CD) with FITC-conjugated monoclonal antibodies (FITC-McAb). [Method] The McAb against CDV, designated as CE3, was purified with protein G and labeled with FITC through agitation method. After purification and identification, the optimal working concentration of FITC-labeled CE3 was determined. Then 61 clinical samples of suspected canine distemper were detected by direct immunofluorescence assay. [Result] The absorption test, blocking test and specificity test showed that the labeled antibody had high specificity and sensitivity, but didnt have cross reaction with canine parvovirus (CPV), canine parainfluenza virus (CPIV), canine adenovirus (CAV) and rabies virus (RV). The optimal working concentration was 1∶80. The positive rate of clinical suspected samples was 48%. [Conclusion] The direct immunofluorescence assay developed in this study was rapid, specific and convenient, and had great significance for the early diagnosis of canine distemper.

  4. 扁浒苔(Ulva compressa)的分子鉴定及生活史的初步研究%Molecular identification of Ulva compressa and preliminary study on its life history

    Institute of Scientific and Technical Information of China (English)

    蔡永超; 马家海; 高嵩; 杜晶; 王丁晶

    2013-01-01

    对绿潮的主要组成种类之一扁浒苔进行了分子鉴定和生活史的初步研究。ITS序列分析结果显示,12个样品聚为3个类群,即Ulva-linza-procera-prolifera (LPP)复合体类群(7个样品)、Ulva compressa类群(4个样品)以及Ulva flexuosa类群(1个样品)。扁浒苔的生活史主要包括单性生殖、无性生殖和有性生殖3种方式,单性生殖最为常见,有性生殖相对较少。%In this research,based on molecular identification,we studied the life history of Ulva compressa,one of main contributing species for the green tide. ITS sequence analysis showed that 12 samples were clustered into 3 different cladesUlva linza-procera-prolifera(LPP)(7 samples),Ulva compressa(4 samples)and Ulva flexuosa(1 sample). Three different reproduction modes of Ulva compressa were observed parthenogenesis, asexual and sexual reproduction, among which parthenogenesis was the most common mode and sexual reproduction was relatively less.

  5. 9 CFR 351.17 - Identification required.

    Science.gov (United States)

    2010-01-01

    ... is made, certifying that the product has been obtained by rendering raw materials derived from... INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation... being exported directly from a certified plant or transferred between certified plants for storage...

  6. Isolation,Screening and Preliminary Identification of Biocontrol Strains of Bacillus spp.%生防芽孢杆菌的分离筛选与初步鉴定

    Institute of Scientific and Technical Information of China (English)

    任士伟; 邢小霞; 董向丽

    2011-01-01

    30 Strains of Bacillus spp. were isolated from 7 soil samples using repeated plate streaking, and 4 strains of them had rivalry ability to Fusarium oxysporum f. sp. cucumerinum. They had also antagonism effect and broad spectrum to other plant pathogenic fungi proved by the method of plate test. We detected that the secondary metabolites of Bacillus spp. had fungistasis and could induce deformed growth of hyphae, the metabolites had water-solubility ability. The hyphae of Fusarium oxysporum f. sp. cucumerinum et al. could be induced bulge, browning, tip dulled. produced sporule by zymotic fluid. A strain of Bacillus subtilis was preliminary identified through observation of its morphological features and culture characters and a series of biochemical experiments.%从不同环境中采集7份土壤样品用于芽孢杆菌的分离纯化,通过多次平板划线分离纯化得到30个菌落形态不同的菌株.以黄瓜枯萎病菌为指示菌对得到的菌株进行抑菌活性筛选,其中的4个菌株具有明显的抑菌作用.采用对峙生长法,测定4株芽孢杆菌对其它植物病原真菌的活性,结果显示4个芽孢杆菌均具有广谱抑菌性.进一步试验表明,4株芽孢杆菌的发酵液也具有明显的抑菌作用,说明对病原真菌有抑制作用的物质水溶性较大,大部分释放到发酵液中,并且发酵液可使黄瓜枯萎等植物病原真菌菌丝膨大、褐变、末端钝圆,促进镰刀菌产生小孢子.通过菌落形态特征、系列生化测定对其进行初步鉴定,结果表明其中一株为枯草芽孢杆菌.

  7. SEQUENCE ANALYSIS AND PRELIMINARY IDENTIFICATION OF MOLECULES WITH EF-HAND DOMAIN OF SCHISTOSOMA JAPONICUM%日本血吸虫含EF手性结构域分子序列的分析和初步鉴定

    Institute of Scientific and Technical Information of China (English)

    张艳丽; 贾侃; 李莹; 苑纯秀; 杨健美; 林矫矫; 冯新港

    2011-01-01

    为分离鉴定日本血吸虫含EF手性结构域分子及分析其序列结构特征,首先找到含有EF—hand结构域的分子,按照序列一级结构进行分类,之后进行生物信息学分析。然后用PCR方法以虫卵和成虫cDNA文库为模板扩增分类后的分子,构建重组质粒,诱导蛋白表达并纯化,选取14个纯化蛋白用Western blot进行初步免疫原性鉴定。结果269个原始含EF-hand结构域分子按照序列一级结构分为70个;成功表达和纯化了49个含EF手性分子,进化树分析将其分为9类;Western blot显示,14个纯化蛋白均可被日本血吸虫感染小鼠阳性血清识别。本研究结果为下一步采用这类分子进行动物保护性效果评价以及保护性免疫机制的研究奠定了基础。%To isolate and identify the molecules with EF-hand domain of Schistosoma japonicum and analyse the sequence structural characteristics of them, the molecules with EF-hand domain were firstly found and classified according to the primary structure, and then analyzed by bioinformatics. PCR was used to amplify the classified molecules with the S.japonicum adult and egg cDNA library. The recombinant plasmids were constructed and the protein with EF-hand domain were expressed and purified. Western blot was used to identify the preliminary immunogenicity of 14 purified proteins. Two hundred and sixty nine original molecules with EF-hand domain were divided into 70 molecules. Forty nine molecules with EF-hand domain were expressed and divided into 9 categories by phylogenetic tree. Western blot analysis showed that 14 purified proteins were specifically recognized by the positive serum from mice infected with S.japonicum. The study provided the basis for further study of immune protection of the molecules.

  8. Identification of the base-pairing requirements for repression of hctA translation by the small RNA IhtA leads to the discovery of a new mRNA target in Chlamydia trachomatis.

    Directory of Open Access Journals (Sweden)

    Nicole A Grieshaber

    Full Text Available The non-coding small RNA, IhtA expressed by the obligate intracellular human pathogen Chlamydia trachomatis modulates the translation of HctA, a key protein involved in replicative to infectious cell type differentiation. Using a combination of bioinformatics and mutagenesis we sought to identify the base pairing requirement for functional repression of HctA protein expression, with an eye to applying our findings towards the identification of additional targets. IhtA is predicted to fold into a three stem:loop structure. We found that loop 1 occludes the initiation codon of hctA, while loop 2 and 3 are not required for function. This 7 nucleotide region forms G/C rich interactions surrounding the AUG of hctA. Two additional genes in the chlamydial genome, CTL0322 and CTL0097, contained some elements of the hctA:IhtA recognition sequence. The mRNA of both CTL0322and CTL0097 interacted with IhtA in vitro as measured by biolayer interferometry. However, using a CheZ reporter expression system, IhtA only inhibited the translation of CTL0322. The proposed IhtA recognition site in the CTL0322 message contains significant G/C base pairing on either side of the initiation codon while CTL0097 only contains G/C base pairing 3' to the AUG initiation codon. These data suggest that as the functional interacting region is only 6-7nt in length that full translation repression is dependent on the degree of G/C base pairing. Additionally our results indicate that IhtA may regulate multiple mRNAs involved in the chlamydial infectious cycle.

  9. Identification of the Base-Pairing Requirements for Repression of hctA Translation by the Small RNA IhtA Leads to the Discovery of a New mRNA Target in Chlamydia trachomatis

    Science.gov (United States)

    Grieshaber, Nicole A.; Tattersall, Jeremiah S.; Liguori, Johella; Lipat, Joseph N.; Runac, Justin; Grieshaber, Scott S.

    2015-01-01

    The non-coding small RNA, IhtA expressed by the obligate intracellular human pathogen Chlamydia trachomatis modulates the translation of HctA, a key protein involved in replicative to infectious cell type differentiation. Using a combination of bioinformatics and mutagenesis we sought to identify the base pairing requirement for functional repression of HctA protein expression, with an eye to applying our findings towards the identification of additional targets. IhtA is predicted to fold into a three stem:loop structure. We found that loop 1 occludes the initiation codon of hctA, while loop 2 and 3 are not required for function. This 7 nucleotide region forms G/C rich interactions surrounding the AUG of hctA. Two additional genes in the chlamydial genome, CTL0322 and CTL0097, contained some elements of the hctA:IhtA recognition sequence. The mRNA of both CTL0322and CTL0097 interacted with IhtA in vitro as measured by biolayer interferometry. However, using a CheZ reporter expression system, IhtA only inhibited the translation of CTL0322. The proposed IhtA recognition site in the CTL0322 message contains significant G/C base pairing on either side of the initiation codon while CTL0097 only contains G/C base pairing 3’ to the AUG initiation codon. These data suggest that as the functional interacting region is only 6-7nt in length that full translation repression is dependent on the degree of G/C base pairing. Additionally our results indicate that IhtA may regulate multiple mRNAs involved in the chlamydial infectious cycle. PMID:25756658

  10. PhosphoHunter: An Efficient Software Tool for Phosphopeptide Identification

    Directory of Open Access Journals (Sweden)

    Alessandra Tiengo

    2015-01-01

    Full Text Available Phosphorylation is a protein posttranslational modification. It is responsible of the activation/inactivation of disease-related pathways, thanks to its role of “molecular switch.” The study of phosphorylated proteins becomes a key point for the proteomic analyses focused on the identification of diagnostic/therapeutic targets. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS is the most widely used analytical approach. Although unmodified peptides are automatically identified by consolidated algorithms, phosphopeptides still require automated tools to avoid time-consuming manual interpretation. To improve phosphopeptide identification efficiency, a novel procedure was developed and implemented in a Perl/C tool called PhosphoHunter, here proposed and evaluated. It includes a preliminary heuristic step for filtering out the MS/MS spectra produced by nonphosphorylated peptides before sequence identification. A method to assess the statistical significance of identified phosphopeptides was also formulated. PhosphoHunter performance was tested on a dataset of 1500 MS/MS spectra and it was compared with two other tools: Mascot and Inspect. Comparisons demonstrated that a strong point of PhosphoHunter is sensitivity, suggesting that it is able to identify real phosphopeptides with superior performance. Performance indexes depend on a single parameter (intensity threshold that users can tune according to the study aim. All the three tools localized >90% of phosphosites.

  11. Preliminary trial of identification of brain structures in nude mice with green fluorescent protein expression by fluoroscope%荧光镜下界定绿色荧光裸小鼠脑结构部位

    Institute of Scientific and Technical Information of China (English)

    郑达理; 董军; 兰青; 黄强; 钱志远; 李如军; 王爱东; 崔宝乾; 陈延明; 费喜峰; 吴明霞; 霍红梅

    2012-01-01

    目的 探讨荧光镜下界定绿色荧光裸小鼠脑结构部位的可行性及优势所在.方法 取表达绿色荧光蛋白(GFP)的成年裸小鼠脑置于SLY脑切片模具中,切出1mm或0.9 mm厚的脑片,再分别对其进行25 μm厚的冰冻切片.荧光镜下观察切片的形态结构,结合《小鼠脑立体定位图谱》中小鼠脑解剖资料,在荧光镜下界定其解剖结构.观察结束后,全部切片行尼氏染色做对照.结果 依据脑内不同结构间的荧光色差可对其进行辨认:细胞核、尼氏小体密集区及神经束分布区为弱荧光信号;细胞核、尼氏小体密集区周围结构,如嗅球的丛状层、小脑的分子层为强荧光信号.切片经尼氏染色后,体视显微镜下观察到的脑结构与荧光镜观察的结果基本一致. 结论 荧光镜下界定绿色荧光裸小鼠脑结构部位可作为一个实验手段应用于荧光示踪实验的解剖定位研究.%Objective To explore the feasibility and advantage of fluoroscope in identification of brain structures in nude mice with green fluorescent protein (GFP) expression. Methods We laid the whole brain separated from 8-week adult nude mice with GFP expression into SLY mouse brain blocker to produce slices of 1 or 0.9 mm thickness; and then,25 μm-thickness frozen sections were cut.Fluoroscope was employed to observe the morphological structure to define their anatomic structures with reference to The Mouse Brain in Stereotaxic Coordinates compiled by Paxinos. After the observation,these frozen sections were performed Nissi staining for contrast. Results Different structures can be identified by their distinct fluorescence intensity:the dense areas of nuclei,Nissl bodies and nerve tract showed low fluorescence intensity; while the structures around the areas of nuclei and nerve tract,such as,the plexiform layer of olfactory bulb and the molecular layer of cerebella,showed high fluorescence intensity.The fluorescence intensity was

  12. UVISS preliminary visibility analysis

    DEFF Research Database (Denmark)

    Betto, Maurizio

    1998-01-01

    The goal of this work is to obtain a preliminary assessment of the sky visibility for anastronomical telescope located on the express pallet of the International SpaceStation (ISS)} taking into account the major constraints imposed on the instrument by the ISSattitude and structure. Part of the w......The goal of this work is to obtain a preliminary assessment of the sky visibility for anastronomical telescope located on the express pallet of the International SpaceStation (ISS)} taking into account the major constraints imposed on the instrument by the ISSattitude and structure. Part...

  13. Topaz II preliminary safety assessment

    Science.gov (United States)

    Marshall, Albert C.; Standley, Vaughn; Voss, Susan S.; Haskin, Eric

    1993-01-01

    The Strategic Defense Initiative Organization (SDIO) decided to investigate the possibility of launching a Russian Topaz II space nuclear power system. A preliminary safety assessment was conducted to determine whether or not a space mission could be conducted safely and within budget constraints. As part of this assessment, a safety policy and safety functional requirements were developed to guide both the safety assessment and future Topaz II activities. A review of the Russian flight safety program was conducted and documented. Our preliminary safety assessment included a top level event tree, neutronic analysis of normal and accident configurations, an evaluation of temperature coefficients of reactivity, a reentry and disposal analysis, and analysis of postulated launch abort impact accidents, and an analysis of postulated propellant fire and explosion accidents. Based on the assessment, it appears that it will be possible to safely launch the Topaz II system in the U.S. with some possible system modifications. The principal system modifications will probably include design changes to preclude water flooded criticality and to assure intact reentry.

  14. Stardust Interstellar Preliminary Examination (ISPE)

    Science.gov (United States)

    Westphal, A. J.; Allen, C.; Bajt, S.; Basset, R.; Bastien, R.; Bechtel, H.; Bleuet, P.; Borg, J.; Brenker F.; Bridges, J.

    2009-01-01

    In January 2006 the Stardust sample return capsule returned to Earth bearing the first solid samples from a primitive solar system body, C omet 81P/Wild2, and a collector dedicated to the capture and return o f contemporary interstellar dust. Both collectors were approximately 0.1m(exp 2) in area and were composed of aerogel tiles (85% of the co llecting area) and aluminum foils. The Stardust Interstellar Dust Col lector (SIDC) was exposed to the interstellar dust stream for a total exposure factor of 20 m(exp 2-) day during two periods before the co metary encounter. The Stardust Interstellar Preliminary Examination ( ISPE) is a three-year effort to characterize the collection using no ndestructive techniques. The ISPE consists of six interdependent proj ects: (1) Candidate identification through automated digital microsco py and a massively distributed, calibrated search (2) Candidate extr action and photodocumentation (3) Characterization of candidates thro ugh synchrotronbased FourierTranform Infrared Spectroscopy (FTIR), S canning XRay Fluoresence Microscopy (SXRF), and Scanning Transmission Xray Microscopy (STXM) (4) Search for and analysis of craters in f oils through FESEM scanning, Auger Spectroscopy and synchrotronbased Photoemission Electron Microscopy (PEEM) (5) Modeling of interstell ar dust transport in the solar system (6) Laboratory simulations of h ypervelocity dust impacts into the collecting media

  15. 一株具有杀松材线虫活性海洋真菌的筛选和初步鉴定%Screening and Preliminary Identification of One Marine Fungal Strain with Nematicidal Activity Against Barsaphelenchus Xylophilus

    Institute of Scientific and Technical Information of China (English)

    徐中强; 于洁; 刘超; 刘晓宇; 郭道森

    2013-01-01

    为了从分离得到的海洋真菌中筛选并鉴定具有杀松材线虫活性的菌株,本文采用稀释涂布平板法对采自青岛近海海域样品中的真菌进行分离,使用浸渍法对菌株的培养滤液和菌丝破碎液进行杀松材线虫活性测定,同时根据菌落形态、大小、色泽等特点的不同,经进一步纯化得到6个纯菌株,对这些菌株进行杀线活性测定,经初步筛选得到2株具有较强杀线活性的菌株,菌株HZ 11处理28 h时杀线活性最高,其培养滤液和菌丝破碎滤液的线虫校正死亡率分别达到72.6%和84.1%;菌株HZ 12处理32 h时杀线活性最高,其培养滤液和菌丝破碎滤液处理的线虫校正死亡率分别为46.3%和58.8%。选出活性表现最强的菌株HZ 11进行后续实验。实验结果表明,菌株HZ 11为轮枝孢属(Verticillium)的真菌。本研究对由松材线虫引起的松树萎蔫病的生物防治具有重要的理论意义。%In order to screen and identify the fungal strains with nematicidal activity against the pine wood nematode,Barsaphelenchus xylophilus,from the fungi isolated from the marine environment,in this pa-per,the dilution and streak plate method was used to isolate fungal strains from the samples collected from the Yellow Sea near Qingdao.The culture filtrate and mycelial extract of the fungal strains isolated were assayed invitro for nematicidal activity using immersion test.At the same time,based on the coloni-al morphology,size,color and other characteristics,six pure strains were isolated.After preliminary screening,two strains with strong nematicidal activity were obtained.Strain HZ 1 1 showed the highest activity against B.xylophilus.The revised mortalities of B.xylophilus treated with the culture filtrate and mycelial extract of HZ 1 1 for 28h were 72.6% and 84.1%,respectively.Strain HZ 1 2 reached the highest nematicidal activity against B.xylophilus treated with the culture filtrate and mycelial

  16. Screen and Preliminary Identification of Lactic acid bacteria to Produce Broad-Spectrum Bacteriocin%产广谱细菌素乳酸菌CW3的筛选和初步鉴定

    Institute of Scientific and Technical Information of China (English)

    吕好新; 王巍东; 谈重芳; 杨飞飞; 焦迎春; 王雁萍; 李宗伟

    2013-01-01

    [Objective] CW3 strain was screened and preliminarily identified.[Method] Oxford cup double plate method was adopted to primarily screen spectrum bacteriocin,then excluding acid and hydrogen peroxide disturbance,the protein property of anti-bacteria material was detected,the strain of rescreening was identified.[Result] The supernatant of CW3 strain can inhibit the growth of indicator strains excluded hydrogen peroxide and organic acid.The inhibitive activity decreased largely after treatment with trpsin and pepsin,which can draw that the anti-microbial substances were bacteriocin.The results of identification of physiology and biochemistry preliminarily identified that CW3 strain was a Lactobacillus plantarum.[Conclusion] CW3 strain was a Lactobacillus plantarum,which can generate spectrum bacteriocin.%[目的]对CW3菌株进行筛选,并且进行初步鉴定.[方法]首先采用牛津杯双层平板法进行产广谱细菌素菌株的初筛,再将初筛得到的菌株进行排除酸和过氧化氢干扰,并检测抑菌物质的蛋白质性质,最终对复筛得到的菌株进行鉴定.[结果]试验得出,排除有机酸、过氧化氢等干扰因素后,发酵液仍有抑菌作用;用胰蛋白酶和胃蛋白酶处理后,发酵液抑菌活性急剧下降,确定产生的抑菌物质具有蛋白质性质,是一类细菌素.经过生理生化试验初步鉴定菌株CW3为植物乳杆菌.[结论]菌株CW3是一种能产广谱细菌素的植物乳杆菌.

  17. 物联网应用中有源RFID标签的EAL4安全要求%Assurance level 4 security requirements of active radio frequency identification tags in the Internet of Things

    Institute of Scientific and Technical Information of China (English)

    高金萍; 石竑松; 王宇航; 杨永生; 张翀斌

    2012-01-01

    A framework is given for evaluation assurance level 4(EAL4) security requirements for radio frequency identification(RFID) active tags in applications for the Internet of Things(IOT).The evaluation assurance level and the security assurance requirements are chosen based on ISO/IEC standard 15 408 and an analysis of the asset value and potential threat.Then,the potential threats are related to the active tags to set security objectives for the target to resist potential attacks.A minimal set of security function requirements is given to specify the functionality of active tags.Finally,the consistency between the Abstractions is analyzed to justify the rationale behind the framework.This work provides a reference for designers of security mechanisms for active tags and a basis for the evaluation and procurement of active tags.%该文为物联网应用中安全要求较高的有源RFID标签类产品建立了一套满足评估保证级(EAL)4级的一组安全要求。此安全要求的建立,以通用评估准则(ISO15408)为依据,通过对有源标签保护的资产价值和潜在威胁的分析,为有源标签界定了合理的评估保证级别及相应的安全保证要求,并通过分析其在物联网应用中可能面临的潜在威胁,导出了有源标签需要满足的安全目的,进一步得出了需要满足的最小安全功能要求,并论证了它们之间的对应性。建立的安全要求框架可以作为指引研发者对有源标签进行全面安全性设计考量的重要参考,也可以为有源标签类产品的采购、测评提供依据。

  18. UVISS preliminary visibility analysis

    DEFF Research Database (Denmark)

    Betto, Maurizio

    1998-01-01

    The goal of this work is to obtain a preliminary assessment of the sky visibility for anastronomical telescope located on the express pallet of the International SpaceStation (ISS)} taking into account the major constraints imposed on the instrument by the ISSattitude and structure. Part...

  19. Draft Function Allocation Framework and Preliminary Technical Basis for Advanced SMR Concepts of Operations

    Energy Technology Data Exchange (ETDEWEB)

    Jacques Hugo; John Forester; David Gertman; Jeffrey Joe; Heather Medema; Julius Persensky; April Whaley

    2013-04-01

    This report presents preliminary research results from the investigation in to the development of new models and guidance for concepts of operations (ConOps) in advanced small modular reactor (aSMR) designs. In support of this objective, three important research areas were included: operating principles of multi-modular plants, functional allocation models and strategies that would affect the development of new, non-traditional concept of operations, and the requiremetns for human performance, based upon work domain analysis and current regulatory requirements. As part of the approach for this report, we outline potential functions, including the theoretical and operational foundations for the development of a new functional allocation model and the identification of specific regulatory requirements that will influence the development of future concept of operations. The report also highlights changes in research strategy prompted by confirmationof the importance of applying the work domain analysis methodology to a reference aSMR design. It is described how this methodology will enrich the findings from this phase of the project in the subsequent phases and help in identification of metrics and focused studies for the determination of human performance criteria that can be used to support the design process.

  20. Transportation System Requirements Document

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    This Transportation System Requirements Document (Trans-SRD) describes the functions to be performed by and the technical requirements for the Transportation System to transport spent nuclear fuel (SNF) and high-level radioactive waste (HLW) from Purchaser and Producer sites to a Civilian Radioactive Waste Management System (CRWMS) site, and between CRWMS sites. The purpose of this document is to define the system-level requirements for Transportation consistent with the CRWMS Requirement Document (CRD). These requirements include design and operations requirements to the extent they impact on the development of the physical segments of Transportation. The document also presents an overall description of Transportation, its functions, its segments, and the requirements allocated to the segments and the system-level interfaces with Transportation. The interface identification and description are published in the CRWMS Interface Specification.

  1. KALIMER preliminary conceptual design report

    Energy Technology Data Exchange (ETDEWEB)

    Hahn, Do Hee; Kim, Y. J.; Kim, Y. G. and others

    2000-08-01

    This report, which summarizes the result of preliminary conceptual design activities during Phase 1, follows the format of safety analysis report. The purpose of publishing this report is to gather all of the design information developed so far in a systematic way so that KALIMER designers have a common source of the consistent design information necessary for their future design activities. This report will be revised and updated as design changes occur and more detailed design specification is developed during Phase 2. Chapter 1 describes the KALIMER Project. Chapter 2 includes the top level design requirements of KALIMER and general plant description. Chapter 3 summarizes the design of structures, components, equipment and systems. Specific systems and safety analysis results are described in the remaining chapters. Appendix on the HCDA evaluation is attached at the end of this report.

  2. The orodental status of a group of elderly in-patients: a preliminary assessment.

    Science.gov (United States)

    McNally, L; Gosney, M A; Doherty, U; Field, E A

    1999-12-01

    To provide a preliminary assessment of the orodental status and dental treatment requirements of a group of elderly in-patients. Cross-sectional. Acute Care of the Elderly and Stroke Rehabilitation units at teaching hospitals in Merseyside. 150 patients aged 58 to 94 years, in which a history could be validated at interview. Questionnaire administered by dentist and clinical examination. Registration with a dentist, prosthetic status and difficulties with dentures, denture hygiene and identification marking, dental treatment needs and evidence of mucosal pathology. Only 27% of patients claimed registration with a dentist. Three quarters of the patients were edentulous and 66 patients wore full dentures; 18 had no prostheses. Difficulties were experienced by one quarter of patients with upper dentures, compared with a half of lower denture wearers. Of the dentures available for inspection, 61% had removable soft debris, 66% were left out at night and 75% were cleaned by the patient, whilst on the ward. No dentures had evidence of identification marking. Of the 39 partially dentate patients, 75% required interventive dental treatment. Denture stomatitis was diagnosed in 29% of patients and 19 had evidence of benign mucosal pathology. The orodental status of this group of elderly in-patients was poor, with a high proportion being edentulous. Few were registered with a dentist and denture hygiene was inadequate. Lack of identification marking is a matter of concern. Closer liaison between hospital staff responsible for elderly in-patients is required, to improve the orodental health and quality of life of this medically compromised group of patients.

  3. BACTEC MGIT960系统在分枝杆菌属菌种初步鉴定及药敏中的应用%Application of BACTEC MGIT 960 system in preliminary identification of mycobacterium and antimicrobial susceptibility test

    Institute of Scientific and Technical Information of China (English)

    孙美兰; 陈晓; 华永川

    2011-01-01

    目的 评价BACTEC MGIT 960系统在分枝杆菌属菌种鉴定及药敏中的应用价值.方法 显微镜观察BACTEC MGIT 960系统分离的162株分枝杆菌在液体培养基中的形态,传统生化和核酸检测法同时鉴定分枝杆菌属菌种,分析细菌形态与菌种之间的关系;应用BACTEC MGIT 960系统对分离的141株结核分枝杆菌进行药敏试验.结果 162株分枝杆菌属在MGIT液体培养基中呈索条状、分枝状、点粒状和分散状4种形态;呈索条状132株分枝杆菌属均为结核分枝杆菌,呈点粒状10株和分散状6株分枝杆菌属均为非结核分枝杆菌;对链霉素、异烟肼、利福平和乙胺丁醇的耐药率分别为9.9%、14.2%、9.2%和6.4%,总耐药率18.4%,同时耐利福平和异烟肼的为8.5%;药敏平均检测时间为9.3 d.结论 BACTEC MGIT960系统可快速将分枝杆菌属初步鉴定为结核分枝杆菌和非结核分枝杆菌,能快速准确获得临床一线抗结核药物的药敏结果.%OBJECTIVE To evaluate the application of BACTEC MGIT 960 system in mycobacterium identification and antimicrobial susceptibility test. METHODS The growth forms of 162 strains in liquid media were observed with microscope. Mycobacterium strains were identified by real-time PCR and traditional biochemistry method.The relation between bacteria form and strain categarly was analyzed. The susceptibility testing of Mycobacterium tuberculosis of 4 major antituberculosis drugs were detected with BACTEC MGIT 960 system. RESULTS Four growth forms of cording, clump and branch, load and scatter, and coccoid form were observed from 162 strains in liquid media. Among these, 132 strains of cording form were all M. tuberculosis, 10 strains of coccoid and 6 strains of load and scatter form were all nontuberculosis mycobacteria. Of the 141 strains mycobacterium tuberculosis complex, the rate of multi-drug resistance (MDR)-TB was 8. 5%, the drug resistance rate of streptomycin, isoniazid, rifampicin and

  4. Molecular identification of green tide algae in the South Yellow Sea and preliminary study on its nutritive value%南黄海绿潮藻的分子鉴定及营养价值初探

    Institute of Scientific and Technical Information of China (English)

    蔡子豪; 杜晶; 孙彬; 何培民; 马家海

    2016-01-01

    对2014年度南黄海绿潮藻进行分子鉴定和营养价值初步研究。 ITS序列分析结果显示,南黄海绿潮藻主要包括Ulva linza-procera-prolifera( LPP)以及Ulva compressa 2个类群。营养价值分析结果显示,南黄海绿潮藻中必需氨基酸与氨基酸总量的比值(EAA/TAA)为30.31%~33.83%,呈味氨基酸含量较高(42.84%~46.89%),脂肪酸组成丰富,其不饱和脂肪酸中α-亚麻酸含量占总脂肪酸的(32.36±6.58)%,蛋白质含量与浙江固着浒苔相近(独立样本t 检验, P >0.05),是一种高蛋白(26.46%~29.01%)、低脂肪(0.76%~0.91%)、富含纤维(4.87%~6.58%)且安全的海藻食品。%Based on molecular identification ,the nutritive value of green tide algae from the South Yellow Sea in 2014 was studied in this research .ITS sequence analysis showed that green tide algae in the South Yellow Sea were include in Ulva linza-procera-prolifera( LPP) and Ulva compressa.Nutritive value analysis indicated that green tide algae in the South Yellow Sea was a new safe and healthy sea food providing higher protein (26.46%-29.01%),lower fat (0.76%-0.91%) and higher dietary fiber (4.87%-6.58%), whose protein content was similar with the atta-ched algae from Zhejiang(independent t-test,P>0.05).The amino acid composition of protein was balance with 30.31%-33.83% for EAA/TAA, and the flavor amino acids contents were high ( 42.84%-46.89%); mean-while, the compositions of fatty acids in green tide algae from the South Yellow Sea were rich ,the contents of alpha linolenic acid was (32.36 ±6.58)%of total fatty acids .The green tide algae was a safe seaweed food with high pro-tein content (26.46%-29.01%), low fat content (0.76%-0.91%) and high fiber content (4.87%-6.58%).

  5. Isolation and Preliminary Identification of Antagonistic Bacterium against Fusarium wilt of Cucumber and Watermelon%黄瓜、西瓜枯萎病拮抗细菌的初步分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    吴洪生; 周晓冬; 李鹤; 刘正柱; 闫霜; 刘小雪; 王增辉; 孔祥云

    2013-01-01

    To screen and isolate the antagonistic bacteria that can control cucumber and watermelon wilt pathogen Fusarium oxysporum from the soil,the antagonistic bacteria from soil samples in the greenhouse where pepper was planted and could antagonize cucumber and watermelon wilt pathogen Fusarium oxysporum were conducted for the plate confrontation experiment.The results showed that,LX6,LX7,LX12,LX13 bacterial strains could antagonize Fusarium oxysporum of cucumber and watermelon and had clear inhibition zone;the inhibition zone diameter arrived at 19.3,18.2,17.4,25.5 mm and 16.2,15.0,15.9,14.8 mm after 96 h.In a 30-day-pot-test,control rates against F.oxysporum of LX6 strain treated in cucumber and watermelon were 85.3 % and 81.2 %,respectively.The results of plate culture,microscopy,16S rDNA-based molecular identification (1650bp fragment) and physiological and biochemical characterization showed that the isolated antagonistic bacterium was Bacillus amyloliquifacience and its molecular weight of 16sDNA fragment was 1650 bp.%为了从土壤中筛选能同时抑制黄瓜、西瓜枯萎病的致病菌尖孢镰刀菌的天然拮抗菌,从种植辣椒的大棚土样中分离培养多种能同时拮抗黄瓜和西瓜枯萎病的拮抗细菌,经平板对峙实验,从辣椒土样中分离出编号为LX6、LX7、LX12、LX13的细菌菌株对黄瓜和西瓜尖孢镰刀菌有明显的拮抗作用,抑菌圈显著,96h抑菌圈直径分别达到19.3、18.2、17.4、25.5 mm和16.2、15.0、15.9、14.8 mm;盆栽试验,拮抗菌LX6对黄瓜和西瓜枯萎病30 d的防治效果分别达到85.3%和81.2%.拮抗效果最显著的LX6菌株做分子生物学的进一步鉴定.经平板培养菌落特征、镜检、16S DNA分子鉴定和生理生化特征鉴定表明,所分离的拮抗菌LX6为淀粉液化芽孢杆菌(Bacillus amyloliquefaciens),16sDNA片段分子量为1650bp.

  6. Secure surface identification codes

    Science.gov (United States)

    Beekhof, F.; Voloshynovskiy, S.; Koval, O.; Villan, R.; Pun, T.

    2008-02-01

    This paper introduces an identification framework for random microstructures of material surfaces. These microstructures represent a kind of unique fingerprints that can be used to track and trace an item as well as for anti-counterfeiting. We first consider the architecture for mobile phone-based item identification and then introduce a practical identification algorithm enabling fast searching in large databases. The proposed algorithm is based on reference list decoding. The link to digital communications and robust perceptual hashing is shown. We consider a practical construction of reference list decoding, which comprizes computational complexity, security, memory storage and performance requirements. The efficiency of the proposed algorithm is demonstrated on experimental data obtained from natural paper surfaces.

  7. Preliminary Identification of Six Antagonistic Actinomycetes in Soil Determination of Its Fungicidal Activities%6株土壤拮抗放线菌的初步鉴定及其抗菌活性测定

    Institute of Scientific and Technical Information of China (English)

    龙建友; 夏建荣

    2011-01-01

    [目的]初步鉴定6株放线菌种类,并测定其发酵产物的抗菌活性.[方法]采用形态学方法鉴定6株放线菌菌株种类,以抑制菌丝生长速率法、孢子萌发法、盆栽和大田试验系统测定6株放线菌菌株发酵产物的杀菌活性.[结果]形态学初步鉴定结果表明,6株菌株属于链霉菌属.生物测定结果表明,500 μg/ml的6株放线菌发酵产物,对棉花枯萎病菌的菌丝生长抑制率均在90%以上;对番茄灰霉病菌、烟草赤星病菌、南瓜枯萎病菌的菌丝生长抑制率也在80%以上.其中,GZ-204和GZ-331菌株发酵产物对小麦根腐病菌和玉米弯孢叶斑病菌的孢子萌发抑制率分别为97.8%、98.2%和99.5%、94.6%.盆栽试验结果表明,GZ-204和GZ-331菌株发酵产物对小麦白粉病的保护效果分别为78.8%和87.1%,治疗效果为62.4%和68.5%.田间试验结果表明,GZ-204和GZ-331菌株发酵200倍稀释液对小麦白粉病的防治效果分别达50.5%,69.2%.[结论]研究结果为新型土壤放线菌源农药的研制奠定了基础.%[ Objective ] The paper was to preliminarily identify the species of six strains of actinomycetes, and to determine the fungicidul activities of their fermentation products. [ Method] The species of six strains of actinomycetes were identified by morphological method, and the fungicidal activities of the fermentation products of six strains of actinomycetes were systematically determined by series of methods including mycelial growth rate inhibition method, spore germination method, pottirg and field test. [ Result] Morphological identification results showed that six strains belonged to streptomyces. Biological determination results indicated that the inhibition rates of the fermentation products of six strains of actinomycetes with the concentration of 500 μg/ml against the mycelial growth of Fusarium oxysporum f. sp. Vasinfectum were all greater than 90%; the inhibition rates against the mycelial growth of Botrytis

  8. 膜基因芯片技术鉴定布鲁杆菌的初步研究%A preliminary study of membrane gene chip assay in screening and species identification of Brucella

    Institute of Scientific and Technical Information of China (English)

    李铁锋; 李坚; 王照; 滕昊林; 王赢; 陈雪娇; 马文丽; 张宝; 桑建国

    2015-01-01

    A,primers were labeled by biotin,target gene was amplified by PCR,and hybridized with the designed gene chip,and finally,biotin was used for identification.Results The length of all 9 strains were all 357 bp,consistent with expected results.The Yersinia pestis O ∶ 9 that had the same antigens with the Brucella showed no specific PCR products.At the 379 and 576 points of gene,the Brucella suis was 379G,576C,the Brucella Abortus was 379T,576T,the Brucella Melitensis was 379T,576C,all these results were identical to the designed probe sequence.Experimental verification of membrane gene chip,the Brucella suis showed color at the points of 379G,576C and F,the Brucella Abortus showed color at the points of 379T,576T and F,the Brucella Melitensis showed color at the points of 379T,576C and F.Conclusion Brucella gene chip can be used to identify Brucella rapidly.

  9. Preliminary design package for prototype solar heating system

    Science.gov (United States)

    1978-01-01

    A summary is given of the preliminary analysis and design activity on solar heating systems. The analysis was made without site specific data other than weather; therefore, the results indicate performance expected under these special conditions. Major items include system candidates, design approaches, trade studies and other special data required to evaluate the preliminary analysis and design. The program calls for the development and delivery of eight prototype solar heating and cooling systems for installation and operational test.

  10. On Preliminary Breakdown

    Science.gov (United States)

    Beasley, W. H.; Petersen, D.

    2013-12-01

    The preliminary breakdown phase of a negative cloud-to-ground lightning flash was observed in detail. Observations were made with a Photron SA1.1 high-speed video camera operating at 9,000 frames per second, fast optical sensors, a flat-plate electric field antenna covering the SLF to MF band, and VHF and UHF radio receivers with bandwidths of 20 MHz. Bright stepwise extensions of a negative leader were observed at an altitude of 8 km during the first few milliseconds of the flash, and were coincident with bipolar electric field pulses called 'characteristic pulses'. The 2-D step lengths of the preliminary processes were in excess of 100 meters, with some 2-D step lengths in excess of 200 meters. Smaller and shorter unipolar electric field pulses were superposed onto the bipolar electric field pulses, and were coincident with VHF and UHF radio pulses. After a few milliseconds, the emerging negative stepped leader system showed a marked decrease in luminosity, step length, and propagation velocity. Details of these events will be discussed, including the possibility that the preliminary breakdown phase consists not of a single developing lightning leader system, but of multiple smaller lightning leader systems that eventually join together into a single system.

  11. Facial image identification using Photomodeler

    DEFF Research Database (Denmark)

    Lynnerup, Niels; Andersen, Marie; Lauritsen, Helle Petri

    2003-01-01

    consist of many images of the same person taken from different angles. We wanted to see if it was possible to combine such a suite of images in useful 3-D renderings of facial proportions.Fifteen male adults were photographed from four different angles. Based on these photographs, a 3-D wireframe model......We present the results of a preliminary study on the use of 3-D software (Photomodeler) for identification purposes. Perpetrators may be photographed or filmed by surveillance systems. The police may wish to have these images compared to photographs of suspects. The surveillance imagery will often...

  12. Biologically Inspired Visual Model With Preliminary Cognition and Active Attention Adjustment.

    Science.gov (United States)

    Qiao, Hong; Xi, Xuanyang; Li, Yinlin; Wu, Wei; Li, Fengfu

    2015-11-01

    Recently, many computational models have been proposed to simulate visual cognition process. For example, the hierarchical Max-Pooling (HMAX) model was proposed according to the hierarchical and bottom-up structure of V1 to V4 in the ventral pathway of primate visual cortex, which could achieve position- and scale-tolerant recognition. In our previous work, we have introduced memory and association into the HMAX model to simulate visual cognition process. In this paper, we improve our theoretical framework by mimicking a more elaborate structure and function of the primate visual cortex. We will mainly focus on the new formation of memory and association in visual processing under different circumstances as well as preliminary cognition and active adjustment in the inferior temporal cortex, which are absent in the HMAX model. The main contributions of this paper are: 1) in the memory and association part, we apply deep convolutional neural networks to extract various episodic features of the objects since people use different features for object recognition. Moreover, to achieve a fast and robust recognition in the retrieval and association process, different types of features are stored in separated clusters and the feature binding of the same object is stimulated in a loop discharge manner and 2) in the preliminary cognition and active adjustment part, we introduce preliminary cognition to classify different types of objects since distinct neural circuits in a human brain are used for identification of various types of objects. Furthermore, active cognition adjustment of occlusion and orientation is implemented to the model to mimic the top-down effect in human cognition process. Finally, our model is evaluated on two face databases CAS-PEAL-R1 and AR. The results demonstrate that our model exhibits its efficiency on visual recognition process with much lower memory storage requirement and a better performance compared with the traditional purely computational

  13. Cluster identification based on correlations.

    Science.gov (United States)

    Schulman, L S

    2012-04-01

    The problem addressed is the identification of cooperating agents based on correlations created as a result of the joint action of these and other agents. A systematic method for using correlations beyond second moments is developed. The technique is applied to a didactic example, the identification of alphabet letters based on correlations among the pixels used in an image of the letter. As in this example, agents can belong to more than one cluster. Moreover, the identification scheme does not require that the patterns be known ahead of time.

  14. Missing persons genetic identification

    Directory of Open Access Journals (Sweden)

    Matija Bajželj

    2017-09-01

    Full Text Available This article presents identification of missing persons from badly preserved post-mortem remains using molecular genetics methods. Extremely polymorphic and individually specific genetic markers that enable the identification of missing persons are microsatellites on autosomal chromosomes, microsatellites on Y chromosome and control region of mitochondrial DNA. For genetic profile comparison, biological material from post-mortem remains and reference samples have to be collected. If post-mortem remains are found shortly after the presumed death of the missing person, their personal items are used for comparison. If these are not available, (the missing person‘s relatives could be used as reference samples or achieved tissues stored in medical institutions if biopsy for the needs of medical diagnostics was performed earlier during their life. When reference samples are not available, genetic identification is not possible. The type of biological material sampled from the deceased depends on the condition of human remains. Blood, soft tissues, nails, teeth or bones are most commonly used for genetic identification, and the time required for DNA extraction depends on the type of biological material. The most demanding and time consuming is extraction of DNA from teeth and bones, therefore we use it in cases when only skeleton is available or we cannot get a sufficient amount of DNA for genetic identification from other tissues. If the genetic profile of post-mortem reamains and a reference sample of the missing person match, the strength of genetic evidence has to be statistically evaluated and the probability of identification reported.

  15. Environmental Survey preliminary report

    Energy Technology Data Exchange (ETDEWEB)

    1988-04-01

    This report presents the preliminary findings from the first phase of the Environmental Survey of the United States Department of Energy (DOE) Sandia National Laboratories conducted August 17 through September 4, 1987. The objective of the Survey is to identify environmental problems and areas of environmental risk associated with Sandia National Laboratories-Albuquerque (SNLA). The Survey covers all environmental media and all areas of environmental regulation. It is being performed in accordance with the DOE Environmental Survey Manual. This phase of the Survey involves the review of existing site environmental data, observations of the operations carried on at SNLA, and interviews with site personnel. 85 refs., 49 figs., 48 tabs.

  16. Ruiz Volcano: Preliminary report

    Science.gov (United States)

    Ruiz Volcano, Colombia (4.88°N, 75.32°W). All times are local (= GMT -5 hours).An explosive eruption on November 13, 1985, melted ice and snow in the summit area, generating lahars that flowed tens of kilometers down flank river valleys, killing more than 20,000 people. This is history's fourth largest single-eruption death toll, behind only Tambora in 1815 (92,000), Krakatau in 1883 (36,000), and Mount Pelée in May 1902 (28,000). The following briefly summarizes the very preliminary and inevitably conflicting information that had been received by press time.

  17. Extremely secure identification documents

    Energy Technology Data Exchange (ETDEWEB)

    Tolk, K.M. [Sandia National Labs., Albuquerque, NM (United States); Bell, M. [Sandia National Labs., Livermore, CA (United States)

    1997-09-01

    The technology developed in this project uses biometric information printed on the document and public key cryptography to ensure that an adversary cannot issue identification documents to unauthorized individuals or alter existing documents to allow their use by unauthorized individuals. This process can be used to produce many types of identification documents with much higher security than any currently in use. The system is demonstrated using a security badge as an example. This project focused on the technologies requiring development in order to make the approach viable with existing badge printing and laminating technologies. By far the most difficult was the image processing required to verify that the picture on the badge had not been altered. Another area that required considerable work was the high density printed data storage required to get sufficient data on the badge for verification of the picture. The image processing process was successfully tested, and recommendations are included to refine the badge system to ensure high reliability. A two dimensional data array suitable for printing the required data on the badge was proposed, but testing of the readability of the array had to be abandoned due to reallocation of the budgeted funds by the LDRD office.

  18. Subspace Identification

    DEFF Research Database (Denmark)

    Knudsen, Torben

    2002-01-01

    Subspace identification algorithms are user friendly, numerical fast and stable and they provide a good consistent estimate of the deterministic part of a system. The weak point is the stochastic part. The uncertainty on this part is discussed below and methods to reduce it is derived....

  19. Subspace Identification

    DEFF Research Database (Denmark)

    Knudsen, Torben

    2001-01-01

    Subspace identification algorithms are user friendly, numerical fast and stable and they provide a good consistent estimate of the deterministic part of a system. The weak point is the stochastic part. The uncertainty on this part is discussed below and methods to reduce it is derived....

  20. Preliminary identification of buffet problems in high speed civil transport

    Science.gov (United States)

    Ravindra, Krishnaswamy

    1994-01-01

    In the present study, some effort is made to identify whether empennage buffet is a relevant factor in the design and operation of the High Speed Civil Transport (HSCT). Based on some results of the only operational supersonic transport, Concorde and the innumerable studies that exist on the tail buffet of high performance airplanes, CFD analyses on the HSCT as well as low speed wind tunnel tests on models, it appears as though buffet will be a factor that needs attention in the proper design of empennage structure. Utilizing the existing empirical relation between the reduced frequency of the leading edge vortices and the geometric parameters, it is estimated that the characteristic frequencies of the vortices from the wing cranks are in the range of certain fundamental frequencies of the wing-fuselage-empennage structure. Buffet is believed to be critical during take-off, climb, descent and landing. Computational and experimental data available in open literature indicate coherent vortex flow structure in the empennage region at supersonic cruise speeds. This raises further concern on the fatigue life of the empennage structure. Three second generation supersonic transport designs taken from open literature are briefly compared with the 'empennage buffet' in mind. Future research efforts relating to buffet studies on the HSCT are summarized. A bibliography pertaining to the present research, including relevant studies on the first generation supersonic transport is presented. The effect of rounded wing leading edges on the present frequency estimates needs further study. The effect of engine exhaust on the flow field in the empennage region also needs further study.

  1. Preliminary DNA Identification for the Tsunami Victims in Thailand

    Institute of Scientific and Technical Information of China (English)

    Ya-Jun Deng; Da-Qing Cai; Jian Wang; Huan-Ming Yang; Sheng-Bin Li; Jun Yu; Yuan-Zhe Li; Xiao-Guang Yu; Li Li; Dong-Ying Wu; Jun Zhou; Tian-You Man; Guang Yang; Jiang-Wei Yan

    2005-01-01

    The 2004 Southeast Asia Tsunami killed nearly 5,400 people in Southern Thailand,including foreign tourists and local residents. To recover DNA evidence as much as possible from the seriously decomposed bodies, we explored procedures of sample preparation from both bone and tooth samples as well as both mitochondrial and nuclear markers. Despite having failed to recover enough DNA for nuclear marker typing, we succeeded in obtaining fully informative results for mitochondrial markers (HV1 and HV2) from 258 tooth samples with a success rate of 51% (258/507).Using an organic DNA extraction method coupled with an ultrafiltration step, we obtained 16 STR (including 13 CODIS loci, one sex discrimination locus, and two Identifiler loci) profiles for 834 samples with a success rate of 79% (834/1,062).In addition, by comparing the allelic frequencies between the typed samples as a group and other index populations, we conclude that the Thai tsunami victims are a combined group of several populations. Our results provide valuable evidence and protocols for the future forensic practice.

  2. Preliminary requirements for thermal storage subsystems in solar thermal applications

    Energy Technology Data Exchange (ETDEWEB)

    Copeland, R.J.

    1980-04-01

    Methodologies for the analysis of value and comparing thermal storage concepts are presented. Value is a measure of worth and is determined by the cost of conventional fuel systems. Value data for thermal storage in large solar thermal electric power applications are presented. Thermal storage concepts must be compared when all are performing the same mission. A method for doing that analysis, called the ranking index, is derived. Necessary data to use the methodology are included.

  3. 36 CFR 72.16 - Preliminary Action Program requirements.

    Science.gov (United States)

    2010-07-01

    ... of non-governmental support (neighborhood, voluntary and business) shall also be given. (c) Adoption... and implementation strategies. Description of the priorities set by the local government as related to the deficiencies outlined above, and the strategies used to allocate available resources over time...

  4. Preliminary Survey on Empirical Research Practices in Requirements Engineering

    NARCIS (Netherlands)

    Condori-Fernandez, Nelly; Daneva, Maia; Wieringa, Roelf J.

    2012-01-01

    [Context and Motivation]. Based on published output in the premium RE conferences and journals, we observe a growing body of research using both quantitative and qualitative research methods to help understand which RE technique, process or tool work better in which context. Also, more and more

  5. Preliminary design for a maglev development facility

    Energy Technology Data Exchange (ETDEWEB)

    Coffey, H.T.; He, J.L.; Chang, S.L.; Bouillard, J.X.; Chen, S.S.; Cai, Y.; Hoppie, L.O.; Lottes, S.A.; Rote, D.M. [Argonne National Lab., IL (United States); Zhang, Z.Y. [Polytechnic Univ., Brooklyn, NY (United States); Myers, G.; Cvercko, A. [Sterling Engineering, Westchester, IL (United States); Williams, J.R. [Alfred Benesch and Co., Chicago, IL (United States)

    1992-04-01

    A preliminary design was made of a national user facility for evaluating magnetic-levitation (maglev) technologies in sizes intermediate between laboratory experiments and full-scale systems. A technical advisory committee was established and a conference was held to obtain advice on the potential requirements of operational systems and how the facility might best be configured to test these requirements. The effort included studies of multiple concepts for levitating, guiding, and propelling maglev vehicles, as well as the controls, communications, and data-acquisition and -reduction equipment that would be required in operating the facility. Preliminary designs for versatile, dual 2-MVA power supplies capable of powering attractive or repulsive systems were developed. Facility site requirements were identified. Test vehicles would be about 7.4 m (25 ft) long, would weigh form 3 to 7 metric tons, and would operate at speeds up to 67 m/s (150 mph) on a 3.3-km (2.05-mi) elevated guideway. The facility would utilize modular vehicles and guideways, permitting the substitution of levitation, propulsion, and guideway components of different designs and materials for evaluation. The vehicle would provide a test cell in which individual suspension or propulsion components or subsystems could be tested under realistic conditions. The system would allow economical evaluation of integrated systems under varying weather conditions and in realistic geometries.

  6. Preliminary design for a maglev development facility

    Energy Technology Data Exchange (ETDEWEB)

    Coffey, H.T.; He, J.L.; Chang, S.L.; Bouillard, J.X.; Chen, S.S.; Cai, Y.; Hoppie, L.O.; Lottes, S.A.; Rote, D.M. (Argonne National Lab., IL (United States)); Zhang, Z.Y. (Polytechnic Univ., Brooklyn, NY (United States)); Myers, G.; Cvercko, A. (Sterling Engineering, Westchester, IL (United States)); Williams, J.R. (Alfred Benesch and Co., Chicago, IL (United States))

    1992-04-01

    A preliminary design was made of a national user facility for evaluating magnetic-levitation (maglev) technologies in sizes intermediate between laboratory experiments and full-scale systems. A technical advisory committee was established and a conference was held to obtain advice on the potential requirements of operational systems and how the facility might best be configured to test these requirements. The effort included studies of multiple concepts for levitating, guiding, and propelling maglev vehicles, as well as the controls, communications, and data-acquisition and -reduction equipment that would be required in operating the facility. Preliminary designs for versatile, dual 2-MVA power supplies capable of powering attractive or repulsive systems were developed. Facility site requirements were identified. Test vehicles would be about 7.4 m (25 ft) long, would weigh form 3 to 7 metric tons, and would operate at speeds up to 67 m/s (150 mph) on a 3.3-km (2.05-mi) elevated guideway. The facility would utilize modular vehicles and guideways, permitting the substitution of levitation, propulsion, and guideway components of different designs and materials for evaluation. The vehicle would provide a test cell in which individual suspension or propulsion components or subsystems could be tested under realistic conditions. The system would allow economical evaluation of integrated systems under varying weather conditions and in realistic geometries.

  7. Preliminary design for a MAGLEV development facility

    Science.gov (United States)

    Coffey, H. T.; He, J. L.; Chang, S. L.; Bouillard, J. X.; Chen, S. S.; Cai, Y.; Hoppie, L. O.; Lottes, S. A.; Rote, D. M.; Zhang, Z. Y.

    1992-04-01

    A preliminary design was made of a national user facility for evaluating magnetic-levitation (maglev) technologies in sizes intermediate between laboratory experiments and full-scale systems. A technical advisory committee was established and a conference was held to obtain advice on the potential requirements of operational systems and how the facility might best be configured to test these requirements. The effort included studies of multiple concepts for levitating, guiding, and propelling maglev vehicles, as well as the controls, communications, and data-acquisition and -reduction equipment that would be required in operating the facility. Preliminary designs for versatile, dual 2-MVA power supplies capable of powering attractive or repulsive systems were developed. Facility site requirements were identified. Test vehicles would be about 7.4 m (25 ft) long, would weigh from 3 to 7 metric tons, and would operate at speeds up to 67 m/s (150 mph) on a 3.3-km (2.05-mi) elevated guideway. The facility would utilize modular vehicles and guideways, permitting the substitution of levitation, propulsion, and guideway components of different designs and materials for evaluation. The vehicle would provide a test cell in which individual suspension or propulsion components or subsystems could be tested under realistic conditions. The system would allow economical evaluation of integrated systems under varying weather conditions and in realistic geometries.

  8. The identification of nerotic behavior in industry

    Directory of Open Access Journals (Sweden)

    R. H. Brophey

    1978-11-01

    Full Text Available After pointing out some of the practical problems associated with the identification of the neurotic worker in a workforce, two methods of identification which comply with the requirements of being both scientific and accurate, i.e. (1 clinical identification and (2 psychometric identification are discussed. The strengths and shortcomings of clinical identification, when the findings are required for industrial research purposes are contrasted with certain advantages and disadvantages of psychometric identification. OpsommingSommige van die praktiese probleme in verband met die identifisering van die neurotiese werker word bespreek en twee identifiserings-metodes wat aan die vereistes van wetenskaplikheid en akkuraatheid voldoen nl. (1 kliniese identifisering en (2 psigometriese identifisering word kortliks behandel. Die tekortkominge en sterkpunte van kliniese identifisering, wanneer die bevindings vir bedryfsnavorsing benodig word, word met sekere voordele en nadele van psigometriese identifisering gekontrasteer.

  9. Utility requirements for fusion

    Energy Technology Data Exchange (ETDEWEB)

    Vondrasek, R.J.

    1982-02-01

    This report describes work done and results obtained during performance of Task 1 of a study of Utility Requirements and Criteria for Fusion Options. The work consisted of developing a list of utility requirements for fusion optics containing definition of the requirements and showing their relative importance to the utility industry. The project team members developed a preliminary list which was refined by discussions and literature searches. The refined list was recast as a questionnaire which was sent to a substantial portion of the utility industry in this country. Forty-three questionnaire recipients responded including thirty-two utilities. A workshop was held to develop a revised requirements list using the survey responses as a major input. The list prepared by the workshop was further refined by a panel consisting of vice presidents of the three project team firms. The results of the study indicate that in addition to considering the cost of energy for a power plant, utilities consider twenty-three other requirements. Four of the requirements were judged to be vital to plant acceptability: Plant Capital Cost, Financial Liability, Plant Safety and Licensability.

  10. Mastering system identification in 100 exercises

    CERN Document Server

    Schoukens, J; Rolain, Yves

    2012-01-01

    "This book enables readers to understand system identification and linear system modeling through 100 practical exercises without requiring complex theoretical knowledge. The contents encompass state-of-the-art system identification methods, with both time and frequency domain system identification methods covered, including the pros and cons of each. Each chapter features MATLAB exercises, discussions of the exercises, accompanying MATLAB downloads, and larger projects that serve as potential assignments in this learn-by-doing resource"--

  11. A Preliminary Jupiter Model

    CERN Document Server

    Hubbard, W B

    2016-01-01

    In anticipation of new observational results for Jupiter's axial moment of inertia and gravitational zonal harmonic coefficients from the forthcoming Juno orbiter, we present a number of preliminary Jupiter interior models. We combine results from ab initio computer simulations of hydrogen-helium mixtures, including immiscibility calculations, with a new nonperturbative calculation of Jupiter's zonal harmonic coefficients, to derive a self-consistent model for the planet's external gravity and moment of inertia. We assume helium rain modified the interior temperature and composition profiles. Our calculation predicts zonal harmonic values to which measurements can be compared. Although some models fit the observed (pre-Juno) second- and fourth-order zonal harmonics to within their error bars, our preferred reference model predicts a fourth-order zonal harmonic whose absolute value lies above the pre-Juno error bars. This model has a dense core of about 12 Earth masses, and a hydrogen-helium-rich envelope with...

  12. A Preliminary Jupiter Model

    Science.gov (United States)

    Hubbard, W. B.; Militzer, B.

    2016-03-01

    In anticipation of new observational results for Jupiter's axial moment of inertia and gravitational zonal harmonic coefficients from the forthcoming Juno orbiter, we present a number of preliminary Jupiter interior models. We combine results from ab initio computer simulations of hydrogen-helium mixtures, including immiscibility calculations, with a new nonperturbative calculation of Jupiter's zonal harmonic coefficients, to derive a self-consistent model for the planet's external gravity and moment of inertia. We assume helium rain modified the interior temperature and composition profiles. Our calculation predicts zonal harmonic values to which measurements can be compared. Although some models fit the observed (pre-Juno) second- and fourth-order zonal harmonics to within their error bars, our preferred reference model predicts a fourth-order zonal harmonic whose absolute value lies above the pre-Juno error bars. This model has a dense core of about 12 Earth masses and a hydrogen-helium-rich envelope with approximately three times solar metallicity.

  13. Thermal energy storage in aquifiers: preliminary information

    Energy Technology Data Exchange (ETDEWEB)

    Allen, R.D.

    1979-12-01

    Topics discussed include: conceptual designs; numerical modelling; field experiments; relevant technical information; feasibility studies; preliminary aquifer selection considerations; and preliminary design and operating considerations. (TFD)

  14. Optimized Experiment Design for Marine Systems Identification

    DEFF Research Database (Denmark)

    Blanke, M.; Knudsen, Morten

    1999-01-01

    Simulation of maneuvring and design of motion controls for marine systems require non-linear mathematical models, which often have more than one-hundred parameters. Model identification is hence an extremely difficult task. This paper discusses experiment design for marine systems identification...

  15. PRELIMINARY PROGRAMMED WHOLE—BODY COUNTER

    Institute of Scientific and Technical Information of China (English)

    张少东; 郑文忠; 等

    1995-01-01

    It is capable of giving the initial intakes of radionuclides and the assessment quantitites used in radiation protection according to its measured results of radionuclides in vivo.It is accomplished by providing the software of controlling,interface and internal dose estimation programs to the original iron cabin shielding whole-body counter.The preliminary application shows that its data processing is rapid and correct,and can meet the requirement of rapid internal radioactive contamination monitoring and diagnosing in case of lots of internal contamination subjects happened in nuclear accident.

  16. Preliminary results on nonparametric facial occlusion detection

    Directory of Open Access Journals (Sweden)

    Daniel LÓPEZ SÁNCHEZ

    2016-10-01

    Full Text Available The problem of face recognition has been extensively studied in the available literature, however, some aspects of this field require further research. The design and implementation of face recognition systems that can efficiently handle unconstrained conditions (e.g. pose variations, illumination, partial occlusion... is still an area under active research. This work focuses on the design of a new nonparametric occlusion detection technique. In addition, we present some preliminary results that indicate that the proposed technique might be useful to face recognition systems, allowing them to dynamically discard occluded face parts.

  17. The Preliminaries of a Reference

    DEFF Research Database (Denmark)

    Butler, Graham; Sadl, Urska

    2017-01-01

    by the reasons why the Court after an oral hearing held in the presence of the parties and eight intervening Member States, and after hearing the Advocate General did not deliver one. The comment examines the legal framework, as well as the detailed procedural rules and guidelines that govern the cooperation...... of national courts in the preliminary reference procedure. It highlights the fact that preliminary references can only work when the preliminaries of a reference – the culture of sincere cooperation and litigation, efficient communication and flexible procedural rules – are in place....

  18. Increased error rates in preliminary reports issued by radiology residents working more than 10 consecutive hours overnight.

    Science.gov (United States)

    Ruutiainen, Alexander T; Durand, Daniel J; Scanlon, Mary H; Itri, Jason N

    2013-03-01

    To determine if the rate of major discrepancies between resident preliminary reports and faculty final reports increases during the final hours of consecutive 12-hour overnight call shifts. Institutional review board exemption status was obtained for this study. All overnight radiology reports interpreted by residents on-call between January 2010 and June 2010 were reviewed by board-certified faculty and categorized as major discrepancies if they contained a change in interpretation with the potential to impact patient management or outcome. Initial determination of a major discrepancy was at the discretion of individual faculty radiologists based on this general definition. Studies categorized as major discrepancies were secondarily reviewed by the residency program director (M.H.S.) to ensure consistent application of the major discrepancy designation. Multiple variables associated with each report were collected and analyzed, including the time of preliminary interpretation, time into shift study was interpreted, volume of studies interpreted during each shift, day of the week, patient location (inpatient or emergency department), block of shift (2-hour blocks for 12-hour shifts), imaging modality, patient age and gender, resident identification, and faculty identification. Univariate risk factor analysis was performed to determine the optimal data format of each variable (ie, continuous versus categorical). A multivariate logistic regression model was then constructed to account for confounding between variables and identify independent risk factors for major discrepancies. We analyzed 8062 preliminary resident reports with 79 major discrepancies (1.0%). There was a statistically significant increase in major discrepancy rate during the final 2 hours of consecutive 12-hour call shifts. Multivariate analysis confirmed that interpretation during the last 2 hours of 12-hour call shifts (odds ratio (OR) 1.94, 95% confidence interval (CI) 1.18-3.21), cross

  19. Whale Identification

    Science.gov (United States)

    1991-01-01

    R:BASE for DOS, a computer program developed under NASA contract, has been adapted by the National Marine Mammal Laboratory and the College of the Atlantic to provide and advanced computerized photo matching technique for identification of humpback whales. The program compares photos with stored digitized descriptions, enabling researchers to track and determine distribution and migration patterns. R:BASE is a spinoff of RIM (Relational Information Manager), which was used to store data for analyzing heat shielding tiles on the Space Shuttle Orbiter. It is now the world's second largest selling line of microcomputer database management software.

  20. Hazard identification and characterisation, and dose response assessment of spore forming pathogens in cooked chilled food containing vegetables

    NARCIS (Netherlands)

    Leusden FM van; MGB

    2001-01-01

    A hazard identification and characterisation, including a preliminary dose response assessment, of sporeforming pathogens in cooked chilled food containing vegetables was performed according to the structure and principles for a quantitative microbiological risk assessment as described by the Codex

  1. Stochastic system identification in structural dynamics

    Science.gov (United States)

    Safak, Erdal

    1988-01-01

    Recently, new identification methods have been developed by using the concept of optimal-recursive filtering and stochastic approximation. These methods, known as stochastic identification, are based on the statistical properties of the signal and noise, and do not require the assumptions of current methods. The criterion for stochastic system identification is that the difference between the recorded output and the output from the identified system (i.e., the residual of the identification) should be equal to white noise. In this paper, first a brief review of the theory is given. Then, an application of the method is presented by using ambient vibration data from a nine-story building.

  2. Identification of Larval Pacific Lampreys (Lampetra Tridentata), River Lampreys (L. Ayresi) and Western Brook Lampreys (L. Richardson) and Thermal Requirements of Early Life History Stages of Lampreys : Annual Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Meeuwig, Michael H.

    2003-02-01

    Two fundamental aspects of lamprey biology were examined to provide tools for population assessment and determination of critical habitat needs of Columbia River Basin lampreys (the Pacific lamprey, Lampetra tridentata, and the western brook lamprey, L. richardsoni). In particular: (1) we examined the usefulness of current diagnostic characteristics in identification of larval lampreys, specifically pigmentation patterns, and collected material for development of meristic and morphometric descriptions of early life stages of lampreys, and (2) we examined the effects of temperature on survival and development of early life stages of Columbia River Basin lampreys.

  3. A PRELIMINARY JUPITER MODEL

    Energy Technology Data Exchange (ETDEWEB)

    Hubbard, W. B. [Lunar and Planetary Laboratory, The University of Arizona, Tucson, AZ 85721 (United States); Militzer, B. [Department of Earth and Planetary Science, Department of Astronomy, University of California, Berkeley, CA 94720 (United States)

    2016-03-20

    In anticipation of new observational results for Jupiter's axial moment of inertia and gravitational zonal harmonic coefficients from the forthcoming Juno orbiter, we present a number of preliminary Jupiter interior models. We combine results from ab initio computer simulations of hydrogen–helium mixtures, including immiscibility calculations, with a new nonperturbative calculation of Jupiter's zonal harmonic coefficients, to derive a self-consistent model for the planet's external gravity and moment of inertia. We assume helium rain modified the interior temperature and composition profiles. Our calculation predicts zonal harmonic values to which measurements can be compared. Although some models fit the observed (pre-Juno) second- and fourth-order zonal harmonics to within their error bars, our preferred reference model predicts a fourth-order zonal harmonic whose absolute value lies above the pre-Juno error bars. This model has a dense core of about 12 Earth masses and a hydrogen–helium-rich envelope with approximately three times solar metallicity.

  4. Arapahoe NWR diversion reconstruction : Preliminary

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This is a preliminary plan for a diversion reconstruction for Hubbard #2. Oklahoma #1, Dryer, Hill and Crowder sites on the Arapahoe National Wildlife Refuge.

  5. Preparation of monoclonal antibodies to nuclear matrix proteins of tissues surrounding esophagus cancer and its preliminary identification%食管癌旁组织核基质蛋白单克隆抗体的制备及初步鉴定

    Institute of Scientific and Technical Information of China (English)

    张新梅; 林汉良; 刘乐和; 张昌卿; 钟叔平; 曾金云; 郑克立

    2000-01-01

    目的 制备抗食管癌旁组织核基质蛋白的特异性单抗。方法 SDS-PAGE分析正常食管组织、食管癌旁组织及 食管癌组织核基质蛋白间的差异。用食管癌旁组织核基质蛋白做免疫原免疫Balb/c小鼠,利用杂交瘤技术制备了5株能稳定 分泌单克隆抗体的杂交瘤细胞株,并对9-1-2/D、9-1-7/D 2株细胞作了初步鉴定。结果 免疫组化分析发现9-1-2/D单抗与食管 癌及正常食管组织均有反应,而9-1-7/D单抗只与食管癌组织反应,与正常食管组织无反应。Western Blotting分析显示: 9-1-2/D单抗与电泳图谱上正常食管组织核基质和食管癌旁组织核基质52 kD的蛋白反应,而与食管癌组织核基质55 kD的蛋 白反应。9-1-7/D单抗只与电泳图谱上食管癌旁组织及癌组织核基质46 kD的蛋白反应,与正常食管组织核基质无反应。结论 本实验为食管癌的早期诊断作了初步有意义的探讨。%Objective To prepare anti-nuclear matrix proteins of tissues surrounding esophagus cancer monoclonal anti- bodies. Methods Differences among nuclear matrix proteins (NMPS) of normal esophagus tissues, esophagus cancer tissues and tissues adjacent to cancer were identified by SDS-PAGE. Balb/c mouse were hyperimmunized with NMPS of tissues adja- cent to esophagus cancer. Five mouse lymphocyte hybridoma cell lines were established and preliminary identification of cell lines 9-1-2/D, 9-1-7/D were made. Results Immunohistochemical analysis showed monoclonal antibody 9-1-2/D reacted both with esophagus cancer and normal esophageal tissues; monoclonal antibody 9-1-7/D reacted only with nucleoli of esophagus cancer cells. Western Blotting analysis showed monoclonal antibody 9-1-2/D reacted with 52 kD NMPS of normal esophagus tissues and tissues adjacent to esophagus cancer and reacted with 55 kD NMP of esophagus cancer tissue. Monoclonal anti- body 9-1-7/D reacted only with 46 kD protein of esophagus cancer

  6. Design and preliminary validation of a tool for the simulation of train braking performance

    Institute of Scientific and Technical Information of China (English)

    Luca Pugi; Monica Malvezzi; Susanna Papini; Gregorio Vettori

    2013-01-01

    Train braking performance is important for the safety and reliability of railway systems. The availability of a tool that allows evaluating such performance on the basis of the main train features can be useful for train system designers to choose proper dimensions for and optimize train’s subsystems. This paper presents a modular tool for the prediction of train braking performance, with a par-ticular attention to the accurate prediction of stopping distances. The tool takes into account different loading and operating conditions, in order to verify the safety require-ments prescribed by European technical specifications for interoperability of high-speed trains and the corresponding EN regulations. The numerical results given by the tool were verified and validated by comparison with experi-mental data, considering as benchmark case an Ansaldo EMU V250 train-a European high-speed train-currently developed for Belgium and Netherlands high-speed lines, on which technical information and experimental data directly recorded during the preliminary tests were avail-able. An accurate identification of the influence of the braking pad friction factor on braking performances allowed obtaining reliable results.

  7. Emergency CT brain: preliminary interpretation with a tablet device: image quality and diagnostic performance of the Apple iPad.

    LENUS (Irish Health Repository)

    Mc Laughlin, Patrick

    2012-04-01

    Tablet devices have recently been used in radiological image interpretation because they have a display resolution comparable to desktop LCD monitors. We identified a need to examine tablet display performance prior to their use in preliminary interpretation of radiological images. We compared the spatial and contrast resolution of a commercially available tablet display with a diagnostic grade 2 megapixel monochrome LCD using a contrast detail phantom. We also recorded reporting discrepancies, using the ACR RADPEER system, between preliminary interpretation of 100 emergency CT brain examinations on the tablet display and formal review on a diagnostic LCD. The iPad display performed inferiorly to the diagnostic monochrome display without the ability to zoom. When the software zoom function was enabled on the tablet device, comparable contrast detail phantom scores of 163 vs 165 points were achieved. No reporting discrepancies were encountered during the interpretation of 43 normal examinations and five cases of acute intracranial hemorrhage. There were seven RADPEER2 (understandable) misses when using the iPad display and 12 with the diagnostic LCD. Use of software zoom in the tablet device improved its contrast detail phantom score. The tablet allowed satisfactory identification of acute CT brain findings, but additional research will be required to examine the cause of "understandable" reporting discrepancies that occur when using tablet devices.

  8. 33 CFR 181.31 - Manufacturer identification code assignment.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Manufacturer identification code assignment. 181.31 Section 181.31 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... identification code assignment. (a) Each person required by § 181.23 to affix hull identifications numbers must...

  9. 20 CFR 422.112 - Employer identification numbers.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Employer identification numbers. 422.112... Procedures § 422.112 Employer identification numbers. (a) General. Most employers are required by section...)-1 to obtain an employer identification number (EIN) and to include it on wage reports filed with...

  10. Software requirements

    CERN Document Server

    Wiegers, Karl E

    2003-01-01

    Without formal, verifiable software requirements-and an effective system for managing them-the programs that developers think they've agreed to build often will not be the same products their customers are expecting. In SOFTWARE REQUIREMENTS, Second Edition, requirements engineering authority Karl Wiegers amplifies the best practices presented in his original award-winning text?now a mainstay for anyone participating in the software development process. In this book, you'll discover effective techniques for managing the requirements engineering process all the way through the development cy

  11. Analyzing and Specifying Reusable Security Requirements

    Science.gov (United States)

    2003-09-01

    sections specifying functional requirements. Thus, the functional requirements for an embedded avionics application and an ecommerce website may have...avionics applications and ecommerce applications need to specify levels of identification, authentication, authorization, integrity, privacy, etc. At...consider the following template for specifying integrity requirements: • “The [application / component / data center / business unit] shall protect the

  12. Direction Identification System of Garlic Clove Based on Machine Vision

    Directory of Open Access Journals (Sweden)

    Gao Chi

    2013-05-01

    Full Text Available In order to fulfill the requirements of seeding direction of garlic cloves, the paper proposed a research method of garlic clove direction identification based on machine vision, it expounded the theory of garlic clove direction identification, stated the arithmetic of it, designed the direction identification device of it, then developed the control system of garlic clove direction identification based on machine vision, at last tested the garlic clove direction identification, and the result of the experiment certificated that the rate of garlic clove direction identification could reach to more than 97%, and it demonstrated that the research is of high feasibility and technological values.

  13. Energy requirements

    NARCIS (Netherlands)

    Hulzebos, Christian V.; Sauer, Pieter J. J.

    2007-01-01

    The determination of the appropriate energy and nutritional requirements of a newborn infant requires a clear goal of the energy and other compounds to be administered, valid methods to measure energy balance and body composition, and knowledge of the neonatal metabolic capacities. Providing an appr

  14. Energy requirements

    NARCIS (Netherlands)

    Hulzebos, Christian V.; Sauer, Pieter J. J.

    The determination of the appropriate energy and nutritional requirements of a newborn infant requires a clear goal of the energy and other compounds to be administered, valid methods to measure energy balance and body composition, and knowledge of the neonatal metabolic capacities. Providing an

  15. OSU TOMF Program Site Selection and Preliminary Concept Design Report

    Energy Technology Data Exchange (ETDEWEB)

    Spadling, Steve [Oklahoma State Univ., Stillwater, OK (United States)

    2012-05-10

    The purpose of this report is to confirm the programmatic requirements for the new facilities, identify the most appropriate project site, and develop preliminary site and building concepts that successfully address the overall project goals and site issues. These new facilities will be designed to accommodate the staff, drivers and maintenance requirements for the future mixed fleet of passenger vehicles, Transit Style Buses and School Buses.

  16. Preliminary design data package, appendix C. [hybrid electric vehicles

    Science.gov (United States)

    1979-01-01

    The data and documentation required to define the preliminary design of a near term hybrid vehicle and to quantify its operational characteristics are presented together with the assumptions and rationale behind the design decisions. Aspects discussed include development requirements for the propulsion system, the chassis system, the body, and the vehicle systems. Particular emphasis is given to the controls, the heat engine, and the batteries.

  17. An Automatic Identification Procedure to Promote the use of FES-Cycling Training for Hemiparetic Patients

    Directory of Open Access Journals (Sweden)

    Emilia Ambrosini

    2014-01-01

    Full Text Available Cycling induced by Functional Electrical Stimulation (FES training currently requires a manual setting of different parameters, which is a time-consuming and scarcely repeatable procedure. We proposed an automatic procedure for setting session-specific parameters optimized for hemiparetic patients. This procedure consisted of the identification of the stimulation strategy as the angular ranges during which FES drove the motion, the comparison between the identified strategy and the physiological muscular activation strategy, and the setting of the pulse amplitude and duration of each stimulated muscle. Preliminary trials on 10 healthy volunteers helped define the procedure. Feasibility tests on 8 hemiparetic patients (5 stroke, 3 traumatic brain injury were performed. The procedure maximized the motor output within the tolerance constraint, identified a biomimetic strategy in 6 patients, and always lasted less than 5 minutes. Its reasonable duration and automatic nature make the procedure usable at the beginning of every training session, potentially enhancing the performance of FES-cycling training.

  18. An automatic identification procedure to promote the use of FES-cycling training for hemiparetic patients.

    Science.gov (United States)

    Ambrosini, Emilia; Ferrante, Simona; Schauer, Thomas; Ferrigno, Giancarlo; Molteni, Franco; Pedrocchi, Alessandra

    2014-01-01

    Cycling induced by Functional Electrical Stimulation (FES) training currently requires a manual setting of different parameters, which is a time-consuming and scarcely repeatable procedure. We proposed an automatic procedure for setting session-specific parameters optimized for hemiparetic patients. This procedure consisted of the identification of the stimulation strategy as the angular ranges during which FES drove the motion, the comparison between the identified strategy and the physiological muscular activation strategy, and the setting of the pulse amplitude and duration of each stimulated muscle. Preliminary trials on 10 healthy volunteers helped define the procedure. Feasibility tests on 8 hemiparetic patients (5 stroke, 3 traumatic brain injury) were performed. The procedure maximized the motor output within the tolerance constraint, identified a biomimetic strategy in 6 patients, and always lasted less than 5 minutes. Its reasonable duration and automatic nature make the procedure usable at the beginning of every training session, potentially enhancing the performance of FES-cycling training.

  19. Novel handheld magnetometer probe based on magnetic tunnelling junction sensors for intraoperative sentinel lymph node identification.

    Science.gov (United States)

    Cousins, A; Balalis, G L; Thompson, S K; Forero Morales, D; Mohtar, A; Wedding, A B; Thierry, B

    2015-06-03

    Using magnetic tunnelling junction sensors, a novel magnetometer probe for the identification of the sentinel lymph node using magnetic tracers was developed. Probe performance was characterised in vitro and validated in a preclinical swine model. Compared to conventional gamma probes, the magnetometer probe showed excellent spatial resolution of 4.0 mm, and the potential to detect as few as 5 μg of magnetic tracer. Due to the high sensitivity of the magnetometer, all first-tier nodes were identified in the preclinical experiments, and there were no instances of false positive or false negative detection. Furthermore, these preliminary data encourage the application of the magnetometer probe for use in more complex lymphatic environments, such as in gastrointestinal cancers, where the sentinel node is often in close proximity to other non-sentinel nodes, and high spatial resolution detection is required.

  20. Preliminary design package for prototype solar heating and cooling systems

    Science.gov (United States)

    1978-01-01

    A summary is given of the preliminary analysis and design activity on solar heating and cooling systems. The analysis was made without site specific data other than weather; therefore, the results indicate performance expected under these special conditions. Major items include a market analysis, design approaches, trade studies and other special data required to evaluate the preliminary analysis and design. The program calls for the development and delivery of eight prototype solar heating and cooling systems for installation and operational test. Two heating and six heating and cooling units will be delivered for Single Family Residences, Multiple-family Residences and commercial applications.

  1. Aftershock identification

    CERN Document Server

    Zaliapin, Ilya; Keilis-Borok, Vladimir; Wong, Henry

    2007-01-01

    Earthquake aftershock identification is closely related to the question ``Are aftershocks different from the rest of earthquakes?'' We give a positive answer to this question and introduce a general statistical procedure for clustering analysis of seismicity that can be used, in particular, for aftershock detection. The proposed approach expands the analysis of Baiesi and Paczuski [PRE, 69, 066106 (2004)] based on the space-time-magnitude nearest-neighbor distance $\\eta$ between earthquakes. We show that for a homogeneous Poisson marked point field with exponential marks, the distance $\\eta$ has Weibull distribution, which bridges our results with classical correlation analysis for unmarked point fields. We introduce a 2D distribution of spatial and temporal components of $\\eta$, which allows us to identify the clustered part of a point field. The proposed technique is applied to several synthetic seismicity models and to the observed seismicity of Southern California.

  2. Peptide identification

    Science.gov (United States)

    Jarman, Kristin H [Richland, WA; Cannon, William R [Richland, WA; Jarman, Kenneth D [Richland, WA; Heredia-Langner, Alejandro [Richland, WA

    2011-07-12

    Peptides are identified from a list of candidates using collision-induced dissociation tandem mass spectrometry data. A probabilistic model for the occurrence of spectral peaks corresponding to frequently observed partial peptide fragment ions is applied. As part of the identification procedure, a probability score is produced that indicates the likelihood of any given candidate being the correct match. The statistical significance of the score is known without necessarily having reference to the actual identity of the peptide. In one form of the invention, a genetic algorithm is applied to candidate peptides using an objective function that takes into account the number of shifted peaks appearing in the candidate spectrum relative to the test spectrum.

  3. Repository Subsurface Preliminary Fire Hazard Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Richard C. Logan

    2001-07-30

    This fire hazard analysis identifies preliminary design and operations features, fire, and explosion hazards, and provides a reasonable basis to establish the design requirements of fire protection systems during development and emplacement phases of the subsurface repository. This document follows the Technical Work Plan (TWP) (CRWMS M&O 2001c) which was prepared in accordance with AP-2.21Q, ''Quality Determinations and Planning for Scientific, Engineering, and Regulatory Compliance Activities''; Attachment 4 of AP-ESH-008, ''Hazards Analysis System''; and AP-3.11Q, ''Technical Reports''. The objective of this report is to establish the requirements that provide for facility nuclear safety and a proper level of personnel safety and property protection from the effects of fire and the adverse effects of fire-extinguishing agents.

  4. Filament Identification through Mathematical Morphology

    CERN Document Server

    Koch, Eric W

    2015-01-01

    We present a new algorithm for detecting filamentary structure FilFinder. The algorithm uses the techniques of mathematical morphology for filament identification, presenting a complementary approach to current algorithms which use matched filtering or critical manifolds. Unlike other methods, FilFinder identifies filaments over a wide dynamic range in brightness. We apply the new algorithm to far infrared imaging data of dust emission released by the Herschel Gould Belt Survey team. Our preliminary analysis characterizes both filaments and fainter striations. We find a typical filament width of 0.09 pc across the sample, but the brightness varies from cloud to cloud. Several regions show a bimodal filament brightness distribution, with the bright mode (filaments) being an order of magnitude brighter than the faint mode (striations). Using the Rolling Hough Transform, we characterize the orientations of the striations in the data, finding preferred directions that agree with magnetic field direction where dat...

  5. Requirements dilemma

    OpenAIRE

    2006-01-01

    This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University. Knowing ‘what’ to build is an integral part of an Information System Development, and it is generally understood that this, which is known as Requirements, is achievable through a process of understanding, communication and management. It is currently maintained by the Requirements theorists that successful system design clarifies the interrelations between information and its representations...

  6. Preliminary design of a coffee harvester

    Directory of Open Access Journals (Sweden)

    Raphael Magalhães Gomes Moreira

    2016-10-01

    Full Text Available Design of an agricultural machine is a highly complex process due to interactions between the operator, machine, and environment. Mountain coffee plantations constitute an economic sector that requires huge investments for the development of agricultural machinery to improve the harvesting and post-harvesting processes and to overcome the scarcity of work forces in the fields. The aim of this study was to develop a preliminary design for a virtual prototype of a coffee fruit harvester. In this study, a project methodology was applied and adapted for the development of the following steps: project planning, informational design, conceptual design, and preliminary design. The construction of a morphological matrix made it possible to obtain a list of different mechanisms with specific functions. The union between these mechanisms resulted in variants, which were weighed to attribute scores for each selected criterion. From each designated proposal, two variants with the best scores were selected and this permitted the preparation of the preliminary design of both variants. The archetype was divided in two parts, namely the hydraulically articulated arms and the harvesting system that consisted of the vibration mechanism and the detachment mechanism. The proposed innovation involves the use of parallel rods, which were fixed in a plane and rectangular metal sheet. In this step, dimensions including a maximum length of 4.7 m, a minimum length of 3.3 m, and a total height of 2.15 m were identified based on the functioning of the harvester in relation to the coupling point of the tractor.

  7. Preliminary Conceptual Design for Safety Parameter display System of PGSFR

    Energy Technology Data Exchange (ETDEWEB)

    Jo, Hyunju; Seong, Seunghwan; Kim, Wansu; Kim, Donghoon; Son, Kwangseop; Jang, Gwisook [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-05-15

    A PGSFR (prototype Gen-IV sodium-cooled fast reactor) is under development at KAERI. A safety parameter display system (SPDS) should be designed for licensing the PGSFR in order to cope with the TMI action plan requirements. Thus, a preliminary conceptual design for the SPDS of PGSFR including licensing requirements, intrinsic function, critical variables and alarm legs for the SPDS of the PGSFR is studied herein. At first, some documents issued by NRC related to install a SPDS include the following: - NUREG-0737, Clarification of TMI Action Plan Requirements - NUREG-0696, Functional Criteria for Emergency Response Facilities - NUREG-0835, Human Factors Acceptance Criteria for the Safety Parameter display System, Draft Report for Comment. The preliminary concept design for the SPDS of the PGSFR was studied. In designing of the PGSFR, the studied design concept will be refined and implemented through further studies to acquire the approval of a safety analysis report of the PGSFR.

  8. The development of identification in Freudian theory.

    Science.gov (United States)

    Halperin, S; Shakow, C

    1989-01-01

    Once identification achieved its status as a specific psychoanalytic concept it followed a process of reconceptualization that emerged from the different clinical and theoretical contexts in which Freud approached and explained the phenomenon. In tracing the unfolding of the theory of identification throughout Freud's works, we have accounted for the following steps: First, in his correspondence with Fliess, Freud announced topics that would subsequently be theoretically processed. Second, in the first topography, identification was explored in the contexts of hysteria and dreams, and elaborated through reference to two psychic scenes with their respective modes of psychic functioning. Third, in the period of transition to the second topography identification was defined as a substitute for an object relationship and as a preliminary stage of object choice. Interlocked with the concept of narcissism, it produced a reconceptualization of the ego that led to the second topography. Finally, the tripartite model proposed in the second topography manifests the consolidation of the structuring function of identification, since the psychic structure is therein conceived of as resulting from the vicissitudes of object relationships.

  9. Electron identification capabilities of CBM

    Energy Technology Data Exchange (ETDEWEB)

    Lebedev, Semen [GSI, Darmstadt (Germany)]|[JINR, Dubna (Russian Federation)

    2008-07-01

    The Compressed Baryonic Matter (CBM) experiment at the future FAIR facility at Darmstadt will measure dileptons emitted from the hot and dense phase in heavy-ion collisions. In case of an electron measurement, a high purity of identified electrons is required in order to suppress the background. Electron identification in CBM will be performed by a RICH and TRD detectors. In this contribution we will present routines which have been developed for electron identification in CBM. A RICH ring recognition algorithm based on the Hough Transform has been implemented. An ellipse fitting algorithm has been elaborated because most of the CBM RICH rings have elliptic shapes, moreover, it helps to improve ring-track matching and electron identification procedures. An Artificial Neural Network can be used in order to suppress fake rings. The electron identification in RICH is substantially improved by the use of TRD information for which 3 different algorithms are implemented. Results of primary electron identification are presented. All developed algorithms were tested on large statistics of simulated events and are included into the CBM software framework for common use.

  10. Identification of microcracks caused by autogenous shrinkage

    DEFF Research Database (Denmark)

    Lura, Pietro; Jensen, Ole Mejlhede; Guang, Ye

    2005-01-01

    Detection and quantification of microcracks caused by restrained autogenous shrinkage in high-performance concrete is difficult. Available techniques either lack the required resolution or may cause further cracks indistinguishable from the original ones. The new technique presented in this paper...... microscopy, electron probe micro analysis, EPMA, and scanning electron microscopy, SEM. A preliminary analysis of the observed microcracks is presented in this paper....

  11. In Silico Identification Software (ISIS): A Machine Learning Approach to Tandem Mass Spectral Identification of Lipids

    Energy Technology Data Exchange (ETDEWEB)

    Kangas, Lars J.; Metz, Thomas O.; Isaac, Georgis; Schrom, Brian T.; Ginovska-Pangovska, Bojana; Wang, Luning; Tan, Li; Lewis, Robert R.; Miller, John H.

    2012-05-15

    Liquid chromatography-mass spectrometry-based metabolomics has gained importance in the life sciences, yet it is not supported by software tools for high throughput identification of metabolites based on their fragmentation spectra. An algorithm (ISIS: in silico identification software) and its implementation are presented and show great promise in generating in silico spectra of lipids for the purpose of structural identification. Instead of using chemical reaction rate equations or rules-based fragmentation libraries, the algorithm uses machine learning to find accurate bond cleavage rates in a mass spectrometer employing collision-induced dissocia-tion tandem mass spectrometry. A preliminary test of the algorithm with 45 lipids from a subset of lipid classes shows both high sensitivity and specificity.

  12. National Ignition Facility site requirements

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-07-01

    The Site Requirements (SR) provide bases for identification of candidate host sites for the National Ignition Facility (NIF) and for the generation of data regarding potential actual locations for the facilities. The SR supplements the NIF Functional Requirements (FR) with information needed for preparation of responses to queries for input to HQ DOE site evaluation. The queries are to include both documents and explicit requirements for the potential host site responses. The Sr includes information extracted from the NIF FR (for convenience), data based on design approaches, and needs for physical and organization infrastructure for a fully operational NIF. The FR and SR describe requirements that may require new construction or may be met by use or modification of existing facilities. The SR do not establish requirements for NIF design or construction project planning. The SR document does not constitute an element of the NIF technical baseline.

  13. Defining resilience: A preliminary integrative literature review

    Science.gov (United States)

    Wilt, Bonnie; Long, Suzanna K.; Shoberg, Thomas G.

    2016-01-01

    The term “resilience” is ubiquitous in technical literature; it appears in numerous forms, such as resilience, resiliency, or resilient, and each use may have a different definition depending on the interpretation of the writer. This creates difficulties in understanding what is meant by ‘resilience’ in any given use case, especially in discussions of interdisciplinary research. To better understand this problem, this research constructs a preliminary integrative literature review to map different definitions, applications and calculation methods of resilience invoked within critical infrastructure applications. The preliminary review uses a State-of-the-Art Matrix (SAM) analysis to characterize differences in definition across disciplines and between regions. Qualifying the various usages of resilience will produce a greater precision in the literature and a deeper insight into types of data required for its evaluation, particularly with respect to critical infrastructure calculations and how such data may be analyzed. Results from this SAM analysis will create a framework of key concepts as part of the most common applications for “resilient critical infrastructure” modeling.

  14. Preliminary Hazards Analysis Plasma Hearth Process

    Energy Technology Data Exchange (ETDEWEB)

    Aycock, M.; Coordes, D.; Russell, J.; TenBrook, W.; Yimbo, P. [Science Applications International Corp., Pleasanton, CA (United States)

    1993-11-01

    This Preliminary Hazards Analysis (PHA) for the Plasma Hearth Process (PHP) follows the requirements of United States Department of Energy (DOE) Order 5480.23 (DOE, 1992a), DOE Order 5480.21 (DOE, 1991d), DOE Order 5480.22 (DOE, 1992c), DOE Order 5481.1B (DOE, 1986), and the guidance provided in DOE Standards DOE-STD-1027-92 (DOE, 1992b). Consideration is given to ft proposed regulations published as 10 CFR 830 (DOE, 1993) and DOE Safety Guide SG 830.110 (DOE, 1992b). The purpose of performing a PRA is to establish an initial hazard categorization for a DOE nuclear facility and to identify those processes and structures which may have an impact on or be important to safety. The PHA is typically performed during and provides input to project conceptual design. The PRA then is followed by a Preliminary Safety Analysis Report (PSAR) performed during Title I and II design. This PSAR then leads to performance of the Final Safety Analysis Report performed during construction, testing, and acceptance and completed before routine operation. Radiological assessments indicate that a PHP facility, depending on the radioactive material inventory, may be an exempt, Category 3, or Category 2 facility. The calculated impacts would result in no significant impact to offsite personnel or the environment. Hazardous material assessments indicate that a PHP facility will be a Low Hazard facility having no significant impacts either onsite or offsite to personnel and the environment.

  15. ERIS: preliminary design phase overview

    Science.gov (United States)

    Kuntschner, Harald; Jochum, Lieselotte; Amico, Paola; Dekker, Johannes K.; Kerber, Florian; Marchetti, Enrico; Accardo, Matteo; Brast, Roland; Brinkmann, Martin; Conzelmann, Ralf D.; Delabre, Bernard A.; Duchateau, Michel; Fedrigo, Enrico; Finger, Gert; Frank, Christoph; Rodriguez, Fernando G.; Klein, Barbara; Knudstrup, Jens; Le Louarn, Miska; Lundin, Lars; Modigliani, Andrea; Müller, Michael; Neeser, Mark; Tordo, Sebastien; Valenti, Elena; Eisenhauer, Frank; Sturm, Eckhard; Feuchtgruber, Helmut; George, Elisabeth M.; Hartl, Michael; Hofmann, Reiner; Huber, Heinrich; Plattner, Markus P.; Schubert, Josef; Tarantik, Karl; Wiezorrek, Erich; Meyer, Michael R.; Quanz, Sascha P.; Glauser, Adrian M.; Weisz, Harald; Esposito, Simone; Xompero, Marco; Agapito, Guido; Antichi, Jacopo; Biliotti, Valdemaro; Bonaglia, Marco; Briguglio, Runa; Carbonaro, Luca; Cresci, Giovanni; Fini, Luca; Pinna, Enrico; Puglisi, Alfio T.; Quirós-Pacheco, Fernando; Riccardi, Armando; Di Rico, Gianluca; Arcidiacono, Carmelo; Dolci, Mauro

    2014-07-01

    The Enhanced Resolution Imager and Spectrograph (ERIS) is the next-generation adaptive optics near-IR imager and spectrograph for the Cassegrain focus of the Very Large Telescope (VLT) Unit Telescope 4, which will soon make full use of the Adaptive Optics Facility (AOF). It is a high-Strehl AO-assisted instrument that will use the Deformable Secondary Mirror (DSM) and the new Laser Guide Star Facility (4LGSF). The project has been approved for construction and has entered its preliminary design phase. ERIS will be constructed in a collaboration including the Max- Planck Institut für Extraterrestrische Physik, the Eidgenössische Technische Hochschule Zürich and the Osservatorio Astrofisico di Arcetri and will offer 1 - 5 μm imaging and 1 - 2.5 μm integral field spectroscopic capabilities with a high Strehl performance. Wavefront sensing can be carried out with an optical high-order NGS Pyramid wavefront sensor, or with a single laser in either an optical low-order NGS mode, or with a near-IR low-order mode sensor. Due to its highly sensitive visible wavefront sensor, and separate near-IR low-order mode, ERIS provides a large sky coverage with its 1' patrol field radius that can even include AO stars embedded in dust-enshrouded environments. As such it will replace, with a much improved single conjugated AO correction, the most scientifically important imaging modes offered by NACO (diffraction limited imaging in the J to M bands, Sparse Aperture Masking and Apodizing Phase Plate (APP) coronagraphy) and the integral field spectroscopy modes of SINFONI, whose instrumental module, SPIFFI, will be upgraded and re-used in ERIS. As part of the SPIFFI upgrade a new higher resolution grating and a science detector replacement are envisaged, as well as PLC driven motors. To accommodate ERIS at the Cassegrain focus, an extension of the telescope back focal length is required, with modifications of the guider arm assembly. In this paper we report on the status of the

  16. Preliminary pharmacognostic screening of Achyranthes coynei stem

    Directory of Open Access Journals (Sweden)

    Vinayak Upadhya

    2015-01-01

    Full Text Available Achyranthes coynei is a rare, endemic perennial shrub reported from Karnataka and Maharashtra states of India. The plant is used to treat various disorders by folk healers and was proven to have antimicrobial and antioxidant properties. The present study was undertaken to evaluate microscopic and macroscopic characters of A. coynei stem, along with its physicochemical parameters. ProgRes ® CapturePro and Microsoft Excel were used for statistical analysis. Perennial, shrubby nature and woody stem were the distinguishing morphological characters observed. Transverse section (TS illustrated quadrangular outline of the stem and showed the presence of two types of trichomes on the thick-walled epidermis. TS also showed number of rosette calcium oxalates crystals; prismatic and microsphenoid crystals; conjoint, collateral open secondary vascular bundles; and two amphixylic medullary bundles in the pith. Ash and extractive values, micro and macro elements and nutritive factors were estimated in the present study. The presence of alkaloids, saponins and triterpenoids were observed in preliminary phytochemical screening. High-performance thin layer chromatographic analysis yielded different bands and also indicated the presence of oleanolic acid. The studied parameters for A. coynei stem will be useful for identification and authentication of the plant material.

  17. SciDB versus Spark: A Preliminary Comparison Based on an Earth Science Use Case

    Science.gov (United States)

    Clune, T.; Kuo, K. S.; Doan, K.; Oloso, A.

    2015-12-01

    We compare two Big Data technologies, SciDB and Spark, for performance, usability, and extensibility, when applied to a representative Earth science use case. SciDB is a new-generation parallel distributed database management system (DBMS) based on the array data model that is capable of handling multidimensional arrays efficiently but requires lengthy data ingest prior to analysis, whereas Spark is a fast and general engine for large scale data processing that can immediately process raw data files and thereby avoid the ingest process. Once data have been ingested, SciDB is very efficient in database operations such as subsetting. Spark, on the other hand, provides greater flexibility by supporting a wide variety of high-level tools including DBMS's. For the performance aspect of this preliminary comparison, we configure Spark to operate directly on text or binary data files and thereby limit the need for additional tools. Arguably, a more appropriate comparison would involve exploring other configurations of Spark which exploit supported high-level tools, but that is beyond our current resources. To make the comparison as "fair" as possible, we export the arrays produced by SciDB into text files (or converting them to binary files) for the intake by Spark and thereby avoid any additional file processing penalties. The Earth science use case selected for this comparison is the identification and tracking of snowstorms in the NASA Modern Era Retrospective-analysis for Research and Applications (MERRA) reanalysis data. The identification portion of the use case is to flag all grid cells of the MERRA high-resolution hourly data that satisfies our criteria for snowstorm, whereas the tracking portion connects flagged cells adjacent in time and space to form a snowstorm episode. We will report the results of our comparisons at this presentation.

  18. Preliminary safety design analysis of KALIMER

    Energy Technology Data Exchange (ETDEWEB)

    Suk, Soo Dong; Kwon, Y. M.; Kim, K. D. [Korea Atomic Energy Research Institute, Taejon (Korea)

    1999-03-01

    The national long-term R and D program updated in 1997 requires Korea Atomic Energy Research Institute(KAERI) to complete by the year 2006 the basic design of Korea Advanced Liquid Metal Reactor (KALIMER), along with supporting R and D work, with the capability of resolving the issue of spent fuel storage as well as with significantly enhanced safety. KALIMER is a 150 MWe pool-type sodium cooled prototype reactor that uses metallic fuel. The conceptual design is currently under way to establish a self consistent design meeting a set of the major safety design requirements for accident prevention. Some of current emphasis include those for inherent and passive means of negative reactivity insertion and decay heat removal, high shutdown reliability, prevention of and protection from sodium chemical reaction, and high seismic margin, among others. All of these requirements affect the reactor design significantly and involve supporting R and D programs of substance. This document first introduces a set of safety design requirements and accident evaluation criteria established for the conceptual design of KALIMER and then summarizes some of the preliminary results of engineering and design analyses performed for the safety of KALIMER. 19 refs., 19 figs., 6 tabs. (Author)

  19. Identification of Larval Pacific Lampreys (Lampetra tridentata), River Lampreys (L. ayresi), and Western Brook Lampreys (L. richardsoni) and Thermal Requirements of Early Life History Stages of Lampreys, Annual Report 2002-2003.

    Energy Technology Data Exchange (ETDEWEB)

    Meeuwig, Michael

    2004-01-01

    Two fundamental aspects of lamprey biology were examined to provide tools for population assessment and determination of critical habitat needs of Columbia River Basin (CRB) lampreys (the Pacific lamprey, Lampetra tridentata, and the western brook lamprey, L. richardsoni). We evaluated the usefulness of current diagnostic characteristics for identification of larval lampreys (i.e., pigment patterns) and collected material for development of meristic and morphometric descriptions of early life stage CRB lampreys, and we determined the effects of temperature on survival and development of early life stage CRB lampreys. Thirty-one larval lampreys were collected from locations throughout the CRB and transported to the Columbia River Research Laboratory. Lampreys were sampled at six-week intervals at which time they were identified to the species level based on current diagnostic characteristics. Sampling was repeated until lampreys metamorphosed, at which time species identification was validated based on dentition, or until they died, at which time they were preserved for genetic examination. These lampreys were sampled 30 times with two individuals metamorphosing, both of which were consistently identified, and subsequently validated, as Pacific lampreys. Of the remaining lampreys, only one was inconsistently identified (Pacific lamprey in 83% of the sampling events and western brook lamprey in 17% of the sampling events). These data suggest that pigmentation patterns do not change appreciably through time. In 2001 and 2002 we artificially spawned Pacific and western brook lampreys in the laboratory to provide material for meristic and morphometric descriptions. We collected, digitized, preserved, and measured the mean chorion diameter of Pacific and western brook lamprey embryos. Embryos ranged in development from 1 d post fertilization to just prior to hatch, and were incubated at 14 C. Mean chorion diameter was greater and more variable for Pacific lampreys (mean

  20. Identification of larval Pacific lampreys (Lampetra tridentata), river lampreys (L. ayresi), and western brook lampreys (L. richardsoni) and thermal requirements of early life history stages of lampreys. Annual report 2002-2003

    Science.gov (United States)

    Meeuwig, M.H.; Bayer, J.M.; Seelye, J.G.; Reiche, R.A.

    2003-01-01

    Two fundamental aspects of lamprey biology were examined to provide tools for population assessment and determination of critical habitat needs of Columbia River Basin (CRB) lampreys (the Pacific lamprey, Lampetra tridentata, and the western brook lamprey, L. richardsoni). We evaluated the usefulness of current diagnostic characteristics for identification of larval lampreys (i.e., pigment patterns) and collected material for development of meristic and morphometric descriptions of early life stage CRB lampreys, and we determined the effects of temperature on survival and development of early life stage CRB lampreys. Thirty-one larval lampreys were collected from locations throughout the CRB and transported to the Columbia River Research Laboratory. Lampreys were sampled at six-week intervals at which time they were identified to the species level based on current diagnostic characteristics. Sampling was repeated until lampreys metamorphosed, at which time species identification was validated based on dentition, or until they died, at which time they were preserved for genetic examination. These lampreys were sampled 30 times with two individuals metamorphosing, both of which were consistently identified, and subsequently validated, as Pacific lampreys. Of the remaining lampreys, only one was inconsistently identified (Pacific lamprey in 83% of the sampling events and western brook lamprey in 17% of the sampling events). These data suggest that pigmentation patterns do not change appreciably through time. In 2001 and 2002 we artificially spawned Pacific and western brook lampreys in the laboratory to provide material for meristic and morphometric descriptions. We collected, digitized, preserved, and measured the mean chorion diameter of Pacific and western brook lamprey embryos. Embryos ranged in development from 1 d post fertilization to just prior to hatch, and were incubated at 14 C. Mean chorion diameter was greater and more variable for Pacific lampreys (mean

  1. Automatic Kurdish Dialects Identification

    Directory of Open Access Journals (Sweden)

    Hossein Hassani

    2016-02-01

    Full Text Available Automatic dialect identification is a necessary Lan guage Technology for processing multi- dialect languages in which the dialects are linguis tically far from each other. Particularly, this becomes crucial where the dialects are mutually uni ntelligible. Therefore, to perform computational activities on these languages, the sy stem needs to identify the dialect that is the subject of the process. Kurdish language encompasse s various dialects. It is written using several different scripts. The language lacks of a standard orthography. This situation makes the Kurdish dialectal identification more interesti ng and required, both form the research and from the application perspectives. In this research , we have applied a classification method, based on supervised machine learning, to identify t he dialects of the Kurdish texts. The research has focused on two widely spoken and most dominant Kurdish dialects, namely, Kurmanji and Sorani. The approach could be applied to the other Kurdish dialects as well. The method is also applicable to the languages which are similar to Ku rdish in their dialectal diversity and differences.

  2. Preliminary engineering assessment of the HCLL and HCPB Neutron Activation System

    Energy Technology Data Exchange (ETDEWEB)

    Calderoni, Pattrick; Leichtle, Dieter [Fusion for Energy, Barcelona, (Spain); Angelone, Maurizio [ENEA, Unita Tecnica Fusione, Frascati, (Italy); Klix, Axel [KIT, Eggenstein-Leopoldshafen, (Germany)

    2015-07-01

    The Neutron Activation System (NAS) is one of the four types of neutronics sensors considered for the testing of the HCLL and HCPB Test Blanket Module (TBM) in ITER. It measures the absolute neutron flux intensity with information on the neutron spectrum in selected positions of the TBM. The working principle of the NAS is as follows: the system moves small activation probes (capsules) into selected positions in the TBM (irradiation ends) by means of pneumatic transport with pressurized helium gas; the capsules are irradiated for a selected period, depending on their materials composition (several tens of seconds up to the full plasma pulse length); immediately after the irradiation they are extracted and transported to a gamma spectrometer by means of the same pneumatic transport system; the gamma spectrometer determines the induced gamma activity; the neutron flux and neutron fluence is calculated from the measured gamma activity and the known activation cross section of the materials in the activation probe; after the measurement the capsule is sent either to a disposal or storage (for later measurement). This paper summarizes the results of the feasibility assessment of the TBM NAS in the conceptual design phase, including design justification, identification of requirements based on the expected operating conditions in ITER and preliminary engineering assessment of the activation materials, irradiation ends integration in the modules design and the counting station. (authors)

  3. Preliminary antimicrobial susceptibility: possibility of early and effective therapy during treatment of septic patients

    Directory of Open Access Journals (Sweden)

    Maria Antonietta Distasi

    2014-03-01

    Full Text Available Blood cultures have the drawback of requiring long time to be carried out. Our study was designed with the aim of early reports on both causing agents and preliminary antimicrobial susceptibility. We carried out a direct antimicrobial susceptibility on 79 positive blood cultures (42 for Gram-negative bacteria of which 21 MDRO; 22 for staphylococci of which 15 S. aureus; and 15 Enterococci/Streptococci employing E-test strips deposited on Müller-Hinton Agar for staphylococci and Gram-negative bacteria, and Müller-Hinton Agar + 5% of blood for enterococci/streptococci. MICs were compared to the antimicrobial susceptibility tests carried out using Vitek2. On the basis of the interpretative criteria (SIR, the comparison between the direct antimicrobial susceptibility and the routine antimicrobial susceptibility, showed a concordance from 92.6% up to 100% for Gram-negative bacteria; from 90.0% up to 100% for staphylococci; from 86.7% up to 100% for streptococci/enterococci, respectively. With regard to MICs comparison, good concordance was detected on the basis of ± 1 doubling dilution. Moreover, this work allowed, in the case of Gram-negative bacteria, either the early identification of multidrug-resistant organisms or providing the MIC evaluation in a broader interval than it would be possible by the reference antimicrobial susceptibility.

  4. Bioinformatics analysis of the gene expression profile of hepatocellular carcinoma: preliminary results

    Science.gov (United States)

    Li, Jia

    2016-01-01

    Aim of the study To analyse the expression profile of hepatocellular carcinoma compared with normal liver by using bioinformatics methods. Material and methods In this study, we analysed the microarray expression data of HCC and adjacent normal liver samples from the Gene Expression Omnibus (GEO) database to screen for differentially expressed genes. Then, functional analyses were performed using GenCLiP analysis, Gene Ontology categories, and aberrant pathway identification. In addition, we used the CMap database to identify small molecules that can induce HCC. Results Overall, 2721 differentially expressed genes (DEGs) were identified. We found 180 metastasis-related genes and constructed co-occurrence networks. Several significant pathways, including the transforming growth factor β (TGF-β) signalling pathway, were identified as closely related to these DEGs. Some candidate small molecules (such as betahistine) were identified that might provide a basis for developing HCC treatments in the future. Conclusions Although we functionally analysed the differences in the gene expression profiles of HCC and normal liver tissues, our study is essentially preliminary, and it may be premature to apply our results to clinical trials. Further research and experimental testing are required in future studies. PMID:27095935

  5. CTF3 Design Report Preliminary Phase

    CERN Document Server

    Allard, D; Bernard, M; Bertuzzi, J P; Bienvenu, G; Bonzano, R; Bossart, Rudolf; Braun, H; Bravin, Enrico; Borburgh, J; Buttkus, J; Chazarenc, E; Chaput, R; Chohan, V; Cloye, J J; Corsini, R; Coudert, G; Damiani, M; Deghaye, S; Delahaye, J P; Di Maio, F; Dobers, T; Dubief, P; Dupuy, B; Durieu, L; Ferrari, A; Garvey, Terence; Geschonke, Günther; Hansen, J; Hellgren, H; Hourican, M; Lamidon, M; Le Duff, J; Lefèvre, T; Lewis, J H; Lindroos, J; Mahner, E; McMonagle, G; Monteiro, J; Mourier, J; Mouton, B; Odier, P; Otto, T; Pearce, P; Pittin, R; Poehler, M; Potier, J P; Raich, U; Rettig, M; Rinolfi, Louis; Risselada, Thys; Riva, R; Rossat, G; Royer, P; Sermeus, L; Setas, K; Simonet, G; Sladen, Jonathan P H; Søby, L; Tanner, L; Tecker, F A; Thomi, J C; Wilson, Ian H; Yvon, G

    2001-01-01

    The design of CLIC is based on a two-beam scheme, where the short pulses of high power 30 GHz RF are extracted from a drive beam running parallel to the main beam. The 3rd generation CLIC Test Facility (CTF3) will demonstrate the generation of the drive beam with the appropriate time structure, the extraction of 30 GHz RF power from this beam, as well as acceleration of a probe beam with 30 GHz RF cavities. The project makes maximum use of existing equipment and infrastructure of the LPI complex, which became available after the closure of LEP. In the first stage of the project, the "Preliminary Phase", the existing LIL linac and the EPA ring, both modified to suit the new requirements, are used to investigate the technique of frequency multiplication by means of interleaving bunches from subsequent trains. This report describes the design of this phase.

  6. Preliminary Design of a Femtosecond Oscilloscope

    CERN Document Server

    Gazazyan, Edmond D; Kalantaryan, Davit K; Laziev, Edouard; Margaryan, Amour

    2005-01-01

    The calculations on motion of electrons in a finite length electromagnetic field of linearly and circularly polarized laser beams have shown that one can use the transversal deflection of electrons on a screen at a certain distance after the interaction region for the measurement of the length and longitudinal particle distribution of femtosecond bunches. In this work the construction and preliminary parameters of various parts of a device that may be called femtosecond oscilloscope are considered. The influence of various factors, such as the energy spread and size of the electron bunches, are taken into account. For CO2 laser intensity 1016 W/cm2 and field free drift length 1m the deflection is 5.3 and 0.06 cm, while the few centimeters long interaction length between 2 mirrors requires assembling accuracy 6 mm and 1.3 micron for 20 MeV to 50 keV, respectively.

  7. The odontology victim identification skill assessment system.

    Science.gov (United States)

    Zohn, Harry K; Dashkow, Sheila; Aschheim, Kenneth W; Dobrin, Lawrence A; Glazer, Howard S; Kirschbaum, Mitchell; Levitt, Daniel; Feldman, Cecile A

    2010-05-01

    Mass fatality identification efforts involving forensic odontology can involve hundreds of dental volunteers. A literature review was conducted and forensic odontologists and dental educators consulted to identify lessons learned from past mass fatality identification efforts. As a result, the authors propose a skill assessment system, the Odontology Victim Identification Skill Assessment System (OVID-SAS), which details qualifications required to participate on the Antemortem, Postmortem, Ante/Postmortem Comparison, Field, and Shift Leader/Initial Response Teams. For each qualification, specific skills have been identified along with suggested educational pedagogy and skill assessment methods. Courses and assessments can be developed by dental schools, professional associations, or forensic organizations to teach and test for the skills required for dental volunteers to participate on each team. By implementing a system, such as OVID-SAS, forensic odontologists responsible for organizing and managing a forensic odontology mass fatality identification effort will be able to optimally utilize individuals presenting with proven skills.

  8. 7 CFR 58.446 - Quality requirements.

    Science.gov (United States)

    2010-01-01

    ..., Emmentaler Cheese. (e) Bulk American cheese for manufacturing. The quality requirements for bulk American cheese for manufacturing shall be in accordance with the U.S. Standards for Grades of Bulk American... Products Bearing Usda Official Identification § 58.446 Quality requirements. (a) Cheddar cheese....

  9. 40 CFR 261.147 - Liability requirements.

    Science.gov (United States)

    2010-07-01

    ... consideration of the guarantee. If the guarantor is a firm with a “substantial business relationship” with the... (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE Financial Requirements for Management of Excluded... facility subject to financial assurance requirements under § 261.4(a)(24)(vi)(F) of this chapter, or a...

  10. 6 CFR 37.25 - Renewal of REAL ID driver's licenses and identification cards.

    Science.gov (United States)

    2010-01-01

    ... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.25 Renewal of REAL ID driver's licenses and identification cards. (a) In-person renewals. States must require holders of REAL ID driver's licenses and identification cards to renew...

  11. Automatic computerized radiographic identification of cephalometric landmarks.

    Science.gov (United States)

    Rudolph, D J; Sinclair, P M; Coggins, J M

    1998-02-01

    Computerized cephalometric analysis currently requires manual identification of landmark locations. This process is time-consuming and limited in accuracy. The purpose of this study was to develop and test a novel method for automatic computer identification of cephalometric landmarks. Spatial spectroscopy (SS) is a computerized method that identifies image structure on the basis of a convolution of the image with a set of filters followed by a decision method using statistical pattern recognition techniques. By this method, characteristic features are used to recognize anatomic structures. This study compared manual identification on a computer monitor and the SS automatic method for landmark identification on minimum resolution images (0.16 cm2 per pixel). Minimum resolution (defined as the lowest resolution at which a cephalometric structure could be identified) was used to reduce computational time and memory requirements during this development stage of the SS method. Fifteen landmarks were selected on a set of 14 test images. The results showed no statistical difference (p > 0.05) in mean landmark identification errors between manual identification on the computer display and automatic identification using SS. We conclude that SS shows potential for the automatic detection of landmarks, which is an important step in the development of a completely automatic cephalometric analysis.

  12. Practical Modeling and Comprehensive System Identification of a BLDC Motor

    Directory of Open Access Journals (Sweden)

    Changle Xiang

    2015-01-01

    Full Text Available The aim of this paper is to outline all the steps in a rigorous and simple procedure for system identification of BLDC motor. A practical mathematical model for identification is derived. Frequency domain identification techniques and time domain estimation method are combined to obtain the unknown parameters. The methods in time domain are founded on the least squares approximation method and a disturbance observer. Only the availability of experimental data for rotor speed and armature current are required for identification. The proposed identification method is systematically investigated, and the final identified model is validated by experimental results performed on a typical BLDC motor in UAV.

  13. Practical Damping Identification of FAST Cable Suspension

    Directory of Open Access Journals (Sweden)

    Jinghai Sun

    2014-03-01

    Full Text Available FAST focus cabin is suspended and driven by 6 parallel large span cables. Low stiffness of cables makes the cabin sensitive to disturbance and difficult to control. Structural damping then becomes a key factor that can improve control ability. Therefore, a reasonable damping estimation is important for system design. In this paper, a practical damping identification method is developed based on Ibrahim-time-domain algorithm. The method shows satisfied performance on accuracy and reliability in simulation test and is utilized in vibration experiments to identify damping ratios of both single cable model and FAST 3 m scale cable suspension model. Finally, a preliminary analysis of the damping properties is given out based on the results of identification.

  14. Nonlinear System Identification and Behavioral Modeling

    CERN Document Server

    Huq, Kazi Mohammed Saidul; Kabir, A F M Sultanul

    2010-01-01

    The problem of determining a mathematical model for an unknown system by observing its input-output data pair is generally referred to as system identification. A behavioral model reproduces the required behavior of the original analyzed system, such as there is a one-to-one correspondence between the behavior of the original system and the simulated system. This paper presents nonlinear system identification and behavioral modeling using a work assignment.

  15. Characterization by Mössbauer Spectroscopy and Electron Paramagnetic Resonance of Peruvian Obsidians for Provenance Studies: A Preliminary Study

    Science.gov (United States)

    Bustamante, A.; Montoya, J.; Delgado, M.; Scorzelli, R. B.; Rossi, A. M.; Poupeau, G.

    2003-06-01

    The identification of geological sources of obsidians collected in archaeological sites provides important information about circulation routes and sociocultural relationships during prehistoric periods. This paper presents the preliminary results obtained by MS and EPR on geological obsidians from Ayacucho and Arequipa, in Peru and on archaeological obsidian artifacts from Cerrillos, an early Paracas site of the upper Ica valley, south of Lima (Peru).

  16. Characterization by Moessbauer Spectroscopy and Electron Paramagnetic Resonance of Peruvian Obsidians for Provenance Studies: A Preliminary Study

    Energy Technology Data Exchange (ETDEWEB)

    Bustamante, A.; Montoya, J. [Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Fisicas (Peru); Delgado, M. [QALLTA (Peru); Scorzelli, R. B.; Rossi, A. M. [Centro Brasileiro de Pesquisas Fisicas (Brazil); Poupeau, G. [Institut de Recherche sur les Archeomateriaux, UMR 5060, CNRS/Universite Bordeaux 3, Maison de l' Archeologie, Esplanade des Antilles (France)

    2003-06-15

    The identification of geological sources of obsidians collected in archaeological sites provides important information about circulation routes and sociocultural relationships during prehistoric periods. This paper presents the preliminary results obtained by MS and EPR on geological obsidians from Ayacucho and Arequipa, in Peru and on archaeological obsidian artifacts from Cerrillos, an early Paracas site of the upper Ica valley, south of Lima (Peru).

  17. Preliminary conceptual design about the CEPC calorimeters

    Science.gov (United States)

    Yang, Haijun

    2016-11-01

    The Circular Electron Positron Collider (CEPC) as a Higgs factory was proposed in September 2013. The preliminary conceptual design report was completed in 2015.1 The CEPC detector design was using International Linear Collider Detector — ILD2 as an initial baseline. The CEPC calorimeters, including the high granularity electromagnetic calorimeter (ECAL) and the hadron calorimeter (HCAL), are designed for precise energy measurements of electrons, photons, taus and hadronic jets. The basic resolution requirements for the ECAL and HCAL are about 16%E (GeV) and 50%E (GeV), respectively. To fully exploit the physics potential of the Higgs, W, Z and related Standard Model processes, the jet energy resolution is required to reach 3%-4%, or 30%/E (GeV) at energies below about 100 GeV. To achieve the required performance, a Particle Flow Algorithm (PFA) — oriented calorimetry system is being considered as the baseline design. The CEPC ECAL detector options include silicon-tungsten or scintillator-tungsten structures with analog readout, while the HCAL detector options have scintillator or gaseous detector as the active sensor and iron as the absorber. Some latest R&D studies about ECAL and HCAL within the CEPC working group is also presented.

  18. Guidance for performing preliminary assessments under CERCLA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1991-09-01

    EPA headquarters and a national site assessment workgroup produced this guidance for Regional, State, and contractor staff who manage or perform preliminary assessments (PAs). EPA has focused this guidance on the types of sites and site conditions most commonly encountered. The PA approach described in this guidance is generally applicable to a wide variety of sites. However, because of the variability among sites, the amount of information available, and the level of investigative effort required, it is not possible to provide guidance that is equally applicable to all sites. PA investigators should recognize this and be aware that variation from this guidance may be necessary for some sites, particularly for PAs performed at Federal facilities, PAs conducted under EPA`s Environmental Priorities Initiative (EPI), and PAs at sites that have previously been extensively investigated by EPA or others. The purpose of this guidance is to provide instructions for conducting a PA and reporting results. This guidance discusses the information required to evaluate a site and how to obtain it, how to score a site, and reporting requirements. This document also provides guidelines and instruction on PA evaluation, scoring, and the use of standard PA scoresheets. The overall goal of this guidance is to assist PA investigators in conducting high-quality assessments that result in correct site screening or further action recommendations on a nationally consistent basis.

  19. PRELIMINARY SELECTION OF MGR DESIGN BASIS EVENTS

    Energy Technology Data Exchange (ETDEWEB)

    J.A. Kappes

    1999-09-16

    The purpose of this analysis is to identify the preliminary design basis events (DBEs) for consideration in the design of the Monitored Geologic Repository (MGR). For external events and natural phenomena (e.g., earthquake), the objective is to identify those initiating events that the MGR will be designed to withstand. Design criteria will ensure that radiological release scenarios resulting from these initiating events are beyond design basis (i.e., have a scenario frequency less than once per million years). For internal (i.e., human-induced and random equipment failures) events, the objective is to identify credible event sequences that result in bounding radiological releases. These sequences will be used to establish the design basis criteria for MGR structures, systems, and components (SSCs) design basis criteria in order to prevent or mitigate radiological releases. The safety strategy presented in this analysis for preventing or mitigating DBEs is based on the preclosure safety strategy outlined in ''Strategy to Mitigate Preclosure Offsite Exposure'' (CRWMS M&O 1998f). DBE analysis is necessary to provide feedback and requirements to the design process, and also to demonstrate compliance with proposed 10 CFR 63 (Dyer 1999b) requirements. DBE analysis is also required to identify and classify the SSCs that are important to safety (ITS).

  20. Preliminary design of large reflectors with flat facets

    Science.gov (United States)

    Agrawal, P. K.; Anderson, M. S.; Card, M. F.

    1981-01-01

    A concept for approximating curved antenna surfaces using flat facets is discussed. A preliminary design technique for determining the size of the reflector surface facets necessary to meet antenna surface accuracy requirements is presented. A proposed large microwave radiometer satellite (MRS) is selected as an application, and the far-field electromagnetic response of a faceted reflector surface is compared with that from a spherical reflector surface.

  1. Preliminary Analysis of Helicopter Options to Support Tunisian Counterterrorism Operations

    Science.gov (United States)

    2016-04-27

    results of the current analysis and in Mouton et al., 2015, is the relative cost -effectiveness between the CH-47D and the Mi-17v5. In the previous...helicopters from Sikorsky to fulfill a number of roles in counterterrorism operations. Rising costs and delays in delivery raised the question of...whether other cost -effective options exist to meet Tunisia’s helicopter requirement. Approach Our team conducted a preliminary assessment of

  2. Identification of needs and requirements defined by services subordinated to the Minister of the Interior and Administration in key technology and user interfaces to develop a concept of the Video Signals Integrator (VSI) system

    Science.gov (United States)

    Bukowiecka, Danuta; Tyburska, Agata; Struniawski, Jarosław; Jastrzebski, Pawel; Jewartowski, Blazej; Pozniak, Krzysztof; Kasprowicz, Grzegorz; Pastuszak, Grzegorz; Trochimiuk, Maciej; Abramowski, Andrzej; Gaska, Michal; Frasunek, Przemysław; Nalbach-Moszynska, Małgorzata; Brawata, Sebastian; Bubak, Iwona; Gloza, Małgorzata

    2016-09-01

    Preventing and eliminating the risks of terrorist attacks or natural disasters as well as an increase in the security of mass events and critical infrastructure requires the application of modern technologies. Therefore there is a proposal to construct a tool that integrates video signals transmitted by devices that are a part of video monitoring systems functioning in Poland. The article presents selected results of research conducted by the Police Academy in Szczytno under the implemented project for national defense and security on "Video Signals Integrator" Acronym - VSI. Project Leader: Warsaw University of Technology. The consortium: Police Academy in Szczytno, Atende Software Ltd., VORTEX Ltd. No. DOBBio7/ 01/02/2015 funded by the National Centre for Research and Development.

  3. Identification of a Classical Mutant in the Industrial Host Aspergillus niger by Systems Genetics: LaeA Is Required for Citric Acid Production and Regulates the Formation of Some Secondary Metabolites

    DEFF Research Database (Denmark)

    Niu, Jing; Arentshorst, Mark; Nair, P. Deepa S.

    2015-01-01

    The asexual filamentous fungus Aspergillus niger is an important industrial cell factory for citric acid production. In this study, we genetically characterized a UV-generated A. niger mutant that was originally isolated as a nonacidifying mutant, which is a desirable trait for industrial enzyme...... production. Physiological analysis showed that this mutant did not secrete large amounts of citric acid and oxalic acid, thus explaining the nonacidifying phenotype. As traditional complementation approaches to characterize the mutant genotype were unsuccessful, we used bulk segregant analysis in combination...... was caused by a point mutation in the laeA gene. LaeA encodes a putative methyltransferase-domain protein, which we show here to be required for citric acid production in an A. niger lab strain (N402) and in other citric acid production strains. The unexpected link between LaeA and citric acid production...

  4. A comparison of microscopic and spectroscopic identification methods for analysis of microplastics in environmental samples.

    Science.gov (United States)

    Song, Young Kyoung; Hong, Sang Hee; Jang, Mi; Han, Gi Myung; Rani, Manviri; Lee, Jongmyoung; Shim, Won Joon

    2015-04-15

    The analysis of microplastics in various environmental samples requires the identification of microplastics from natural materials. The identification technique lacks a standardized protocol. Herein, stereomicroscope and Fourier transform infrared spectroscope (FT-IR) identification methods for microplastics (microplastics were significantly (p0.05) different. Depending on the number of samples and the microplastic size range of interest, the appropriate identification method should be determined; selecting a suitable identification method for microplastics is crucial for evaluating microplastic pollution.

  5. Preliminary phytochemical screening, pharmacognostic and physicochemical evalution of leaf of Gmelina arborea

    Institute of Scientific and Technical Information of China (English)

    Daya L Chothani; N M Patel

    2012-01-01

    Objective: The aim of present study was to carry out preliminary phytochemical screening, detailed pharmacognostic profile and physicochemical evaluation of leaf of Gmelina arborea. Methods: Fresh leaf and dried power of the leaves were studied by morphology, microscopy, preliminary phytochemical screening, and florescence analysis of powdered drug. Other physicochemical parameters were also performed as per WHO guide lines. Result: The detailed microscopy revealed that the presence of anomocytic stomata and covering uni-multicellular trichome. Leaf constant such as stomatal number, stomata index, vein islet number, vein termination number were also determined. Physicochemical parameters and florescence analysis were also studied. The preliminary phytochemical screening showed the presence of steroid, triterpenoid, saponin, protein, phenolic compound, flavanoid and carbohydrates. Conclusions:The result of these studies could be useful for correct identification and detection of adulterants of this plant material.

  6. Quantum identification system

    OpenAIRE

    Dusek, Miloslav; Haderka, Ondrej; Hendrych, Martin; Myska, Robert

    1998-01-01

    A secure quantum identification system combining a classical identification procedure and quantum key distribution is proposed. Each identification sequence is always used just once and new sequences are ``refuelled'' from a shared provably secret key transferred through the quantum channel. Two identification protocols are devised. The first protocol can be applied when legitimate users have an unjammable public channel at their disposal. The deception probability is derived for the case of ...

  7. Identificação de requisitos de clientes para o projeto de um dosador de precisão para sementes miúdas Identification of client's requirements to design a precision meter for small seeds

    Directory of Open Access Journals (Sweden)

    Ângelo V. dos Reis

    2006-04-01

    Full Text Available A elevação do custo das sementes miúdas torna necessário o desenvolvimento de semeadoras que tenham controle efetivo sobre a população de plantas, o que pode ser conseguido com a utilização de semeadoras de precisão, as quais, no entanto, não são adequadas aos pequenos espaçamentos entre linhas dessas culturas. Por se tratar de um produto novo, há que se prestar especial atenção ao processo de projeto que será utilizado, de forma a incorporar todos os atributos indispensáveis ao cumprimento da tarefa a que se destina, incluindo-se os de ordem funcional, os de qualidade, os comerciais e os de fabricabilidade. Sendo assim, o presente trabalho tem o objetivo de apresentar os requisitos dos clientes para o projeto de um dosador de precisão para sementes miúdas, identificados com a aplicação de um questionário de campo a agricultores, pesquisadores, vendedores, projetistas, e com informações técnicas obtidas na bibliografia. Foram sintetizadas 40 necessidades dos clientes a partir das respostas dos 55 questionários de campo aplicados e dos dados bibliográficos. A análise dessas necessidades permitiu identificar 27 requisitos de clientes, distribuídos ao longo de diversas fases do ciclo de vida de uma semeadora, para o projeto do produto.The increasing cost of seeds for small grain crops makes it necessary to develop seeders with a more effective control over the stand of plants. This could be accomplished with the use of precision planters, but such equipment is only available for large seed crops. Because this is a new product, especial attention must be paid to the design process to be used, in order to consider all attributes involved in the fulfillment of such task, including those related to the machine functional performance as well as those expressing the product quality, market and production needs. Therefore, this study has the objective of presenting the client's requirements to design a precision meter for

  8. Identification of two Arabidopsis genes encoding a peroxisomal oxidoreductase-like protein and an acyl-CoA synthetase-like protein that are required for responses to pro-auxins.

    Science.gov (United States)

    Wiszniewski, Andrew A G; Zhou, Wenxu; Smith, Steven M; Bussell, John D

    2009-03-01

    Indole-3-butyric acid (IBA) and 2,4-dichlorophenoxybutyric acid (2,4-DB) are metabolised by peroxisomal beta-oxidation to active auxins that inhibit root growth. We screened Arabidopsis mutants for resistance to IBA and 2,4-DB and identified two new 2,4-DB resistant mutants. The mutant genes encode a putative oxidoreductase (SDRa) and a putative acyl-activating enzyme (AAE18). Both proteins are localised to peroxisomes. SDRa is coexpressed with core beta-oxidation genes, but germination, seedling growth and the fatty acid profile of sdra seedlings are indistinguishable from wild type. The sdra mutant is also resistant to IBA, but aae18 is not. AAE18 is the first example of a gene required for response to 2,4-DB but not IBA. The closest relative of AAE18 is AAE17. AAE17 is predicted to be peroxisomal, but an aae17 aae18 double mutant responded similarly to aae18 for all assays. We propose that AAE18 is capable of activating 2,4-DB but IBA activating enzymes remain to be discovered. We present an updated model for peroxisomal pro-auxin metabolism in Arabidopsis that includes SDRa and AAE18.

  9. GRID INFORMATION SECURITY FUNCTIONAL REQUIREMENT

    Directory of Open Access Journals (Sweden)

    Amy Poh Ai Ling

    2011-07-01

    Full Text Available This paper describes the background of smart information infrastructure and the needs for smart grid information security. It introduces the conceptual analysis to the methodology with the application ofhermeneutic circle and information security functional requirement identification. Information security for the grid market cover matters includes automation and communications industry that affects the operation of electric power systems and the functioning of the utilities that manage them and its awareness of this information infrastructure has become critical to the reliability of the power system. Community benefits from of cost savings, flexibility and deployment along with the establishment of wireless communications. However, concern revolves around the security protections for easily accessible devices such as the smart meter and the related communications hardware. On the other hand, the changing points between traditional versus smart grid networking trend and the information security importance on the communication field reflects the criticality of grid information security functional requirement identification. The goal of this paper is to identify the functional requirement and relate its significance addresses to the consumer requirement of an information security of a smart grid. Vulnerabilities may bring forth possibility for an attacker to penetrate a network, make headway admission to control software, alter it to load conditions that destabilize the grid in unpredictable ways. Focusing on the grid information security functional requirement is stepping ahead in developing consumer trust and satisfaction towardsmart grid completeness.

  10. Identification of the critical sequence elements in the cytoplasmic domain of leptin receptor isoforms required for Janus kinase/signal transducer and activator of transcription activation by receptor heterodimers.

    Science.gov (United States)

    Bahrenberg, Gregor; Behrmann, Iris; Barthel, Andreas; Hekerman, Paul; Heinrich, Peter Claus; Joost, Hans-Georg; Becker, Walter

    2002-04-01

    Two predominant splice variants of the leptin receptor (LEPR) are coexpressed in leptin-responsive tissues: the long form, LEPRb, characterized as the signal-transducing receptor, and the signaling-defective short form, LEPRa. It is unknown whether heterodimers of these isoforms are capable of signal transduction via the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. To address this question, chimeric receptors were constructed consisting of the transmembrane and intracellular parts of LEPRb and LEPRa fused with the extracellular domains of either the alpha- or beta-subunit of the IL-5 receptor. This strategy allows the directed heterodimerization of different LEPR cytoplasmic tails and excludes homodimerization. In COS-7 and HEPG2 cells, chimeric receptor heterodimers of LEPRa and LEPRb failed to activate the JAK/STAT pathway, whereas receptor dimers of LEPRb gave rise to the expected ligand-dependent activation of JAK2, phosphorylation of STAT3, and STAT3-dependent promoter activity. Markedly lower amounts of JAK2 were found to be associated with immunoprecipitated LEPRa chimeras than with LEPRb chimeras. Analysis of a series of deletion constructs indicated that a segment of 15 amino acids in addition to the 29 amino acids common to LEPRa and LEPRb was required for partial restoration of JAK/STAT activation. Site-directed mutagenesis of the critical sequence indicated that two hydrophobic residues (Leu896, Phe897) not present in LEPRa were indispensable for receptor signaling. These findings show that LEPRa/LEPRb heterodimers cannot activate STAT3 and identify sequence elements within the LEPR that are critical for the activation of JAK2 and STAT3.

  11. Identification of a Classical Mutant in the Industrial Host Aspergillus niger by Systems Genetics: LaeA Is Required for Citric Acid Production and Regulates the Formation of Some Secondary Metabolites.

    Science.gov (United States)

    Niu, Jing; Arentshorst, Mark; Nair, P Deepa S; Dai, Ziyu; Baker, Scott E; Frisvad, Jens C; Nielsen, Kristian F; Punt, Peter J; Ram, Arthur F J

    2015-11-13

    The asexual filamentous fungus Aspergillus niger is an important industrial cell factory for citric acid production. In this study, we genetically characterized a UV-generated A. niger mutant that was originally isolated as a nonacidifying mutant, which is a desirable trait for industrial enzyme production. Physiological analysis showed that this mutant did not secrete large amounts of citric acid and oxalic acid, thus explaining the nonacidifying phenotype. As traditional complementation approaches to characterize the mutant genotype were unsuccessful, we used bulk segregant analysis in combination with high-throughput genome sequencing to identify the mutation responsible for the nonacidifying phenotype. Since A. niger has no sexual cycle, parasexual genetics was used to generate haploid segregants derived from diploids by loss of whole chromosomes. We found that the nonacidifying phenotype was caused by a point mutation in the laeA gene. LaeA encodes a putative methyltransferase-domain protein, which we show here to be required for citric acid production in an A. niger lab strain (N402) and in other citric acid production strains. The unexpected link between LaeA and citric acid production could provide new insights into the transcriptional control mechanisms related to citric acid production in A. niger. Interestingly, the secondary metabolite profile of a ΔlaeA strain differed from the wild-type strain, showing both decreased and increased metabolite levels, indicating that LaeA is also involved in regulating the production of secondary metabolites. Finally, we show that our systems genetics approach is a powerful tool to identify trait mutations. Copyright © 2016 Niu et al.

  12. Identification of a Classical Mutant in the Industrial Host Aspergillus niger by Systems Genetics: LaeA Is Required for Citric Acid Production and Regulates the Formation of Some Secondary Metabolites

    Directory of Open Access Journals (Sweden)

    Jing Niu

    2016-01-01

    Full Text Available The asexual filamentous fungus Aspergillus niger is an important industrial cell factory for citric acid production. In this study, we genetically characterized a UV-generated A. niger mutant that was originally isolated as a nonacidifying mutant, which is a desirable trait for industrial enzyme production. Physiological analysis showed that this mutant did not secrete large amounts of citric acid and oxalic acid, thus explaining the nonacidifying phenotype. As traditional complementation approaches to characterize the mutant genotype were unsuccessful, we used bulk segregant analysis in combination with high-throughput genome sequencing to identify the mutation responsible for the nonacidifying phenotype. Since A. niger has no sexual cycle, parasexual genetics was used to generate haploid segregants derived from diploids by loss of whole chromosomes. We found that the nonacidifying phenotype was caused by a point mutation in the laeA gene. LaeA encodes a putative methyltransferase-domain protein, which we show here to be required for citric acid production in an A. niger lab strain (N402 and in other citric acid production strains. The unexpected link between LaeA and citric acid production could provide new insights into the transcriptional control mechanisms related to citric acid production in A. niger. Interestingly, the secondary metabolite profile of a ΔlaeA strain differed from the wild-type strain, showing both decreased and increased metabolite levels, indicating that LaeA is also involved in regulating the production of secondary metabolites. Finally, we show that our systems genetics approach is a powerful tool to identify trait mutations.

  13. Optical Identifications of X-ray Selected AGNs

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    For investigating the statistical properties of X-ray selected Active Galactic Nuclei (AGNs), we have carried out a program of optical identification of a selection of X-ray sources from ROSAT All Sky Survey Bright Source Catalogue (RASS-BSC) using the 2.16 m telescope of Beijing Astronomical Observatory (BAO). In the preliminary observations, 23 new AGNs were discovered, of which 9 are quasars, and 14 are Seyfert galaxies.

  14. EQUIPMENTS TO SINGLE PHOTON REGISTRATION. Part 2. SILICON PHOTOMULTIPLIER SIGNAL PRELIMINARY PROCESSING. (Review

    Directory of Open Access Journals (Sweden)

    O. V. Dvornikov

    2013-01-01

    Full Text Available Bias circuits, output signals and readout electronics of silicon photomultipliers are considered. Requirements to analog IC for SiPM signal preliminary processing are formulated. According to requirements the set of radiation hardened analog ICs including the comparator and transresistance amplifiers with different gain and speed is produced.

  15. 16 CFR 303.4 - English language requirement.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false English language requirement. 303.4 Section... AND REGULATIONS UNDER THE TEXTILE FIBER PRODUCTS IDENTIFICATION ACT § 303.4 English language requirement. All required information shall be set out in the English language. If the required...

  16. North Korean refugee doctors' preliminary examination scores

    Directory of Open Access Journals (Sweden)

    Sung Uk Chae

    2016-12-01

    Full Text Available Purpose Although there have been studies emphasizing the re-education of North Korean (NK doctors for post-unification of the Korean Peninsula, study on the content and scope of such re-education has yet to be conducted. Researchers intended to set the content and scope of re-education by a comparative analysis for the scores of the preliminary examination, which is comparable to the Korean Medical Licensing Examination (KMLE. Methods The scores of the first and second preliminary exams were analyzed by subject using the Wilcoxon signed rank test. The passing status of the group of NK doctors for KMLE in recent 3 years were investigated. The multiple-choice-question (MCQ items of which difficulty indexes of NK doctors were lower than those of South Korean (SK medical students by two times of the standard deviation of the scores of SK medical students were selected to investigate the relevant reasons. Results The average scores of nearly all subjects were improved in the second exam compared with the first exam. The passing rate of the group of NK doctors was 75%. The number of MCQ items of which difficulty indexes of NK doctors were lower than those of SK medical students was 51 (6.38%. NK doctors’ lack of understandings for Diagnostic Techniques and Procedures, Therapeutics, Prenatal Care, and Managed Care Programs was suggested as the possible reason. Conclusion The education of integrated courses focusing on Diagnostic Techniques and Procedures and Therapeutics, and apprenticeship-style training for clinical practice of core subjects are needed. Special lectures on the Preventive Medicine are likely to be required also.

  17. Preliminary status of the CALET observations

    Science.gov (United States)

    Torii, Shoji

    2016-07-01

    The CALorimetric Electron Telescope (CALET) space experiment, which has been developed by Japan in collaboration with Italy and the United States, is a high-energy astroparticle physics mission to be installed on the International Space Station (ISS). The primary goals of the CALET mission include investigating possible nearby sources of high energy electrons, studying the details of galactic particle propagation and searching for dark matter signatures. During a two- year mission, extendable to five years, the CALET experiment will measure the flux of cosmic-ray electrons (including positrons) to 20 TeV, gamma-rays to 10 TeV and nuclei with Z=1 to 40 up to several 100 TeV. The instrument consists of two layers of segmented plastic scintillators for the cosmic-ray charge identification (CHD), a 3 radiation length thick tungsten-scintillating fiber imaging calorimeter (IMC) and a 27 radiation length thick lead-tungstate calorimeter (TASC). CALET has sufficient depth, imaging capabilities and excellent energy resolution to allow for a clear separation between hadrons and electrons and between charged particles and gamma rays. The instrument was launched on Aug. 19, 2015 to the ISS with HTV-5 (H-II Transfer Vehicle 5) and was successfully berthed to the Japanese Experiment Module- Exposure Facility (JEM-EF) . After a functional check-out phase until the beginning of October, it started an initial operation phase which was completed on Nov. 17, whence it began its standard operation phase. This paper will review the preliminary status of the CALET.

  18. Identification and stochastic control of helicopter dynamic modes

    Science.gov (United States)

    Molusis, J. A.; Bar-Shalom, Y.

    1983-01-01

    A general treatment of parameter identification and stochastic control for use on helicopter dynamic systems is presented. Rotor dynamic models, including specific applications to rotor blade flapping and the helicopter ground resonance problem are emphasized. Dynamic systems which are governed by periodic coefficients as well as constant coefficient models are addressed. The dynamic systems are modeled by linear state variable equations which are used in the identification and stochastic control formulation. The pure identification problem as well as the stochastic control problem which includes combined identification and control for dynamic systems is addressed. The stochastic control problem includes the effect of parameter uncertainty on the solution and the concept of learning and how this is affected by the control's duel effect. The identification formulation requires algorithms suitable for on line use and thus recursive identification algorithms are considered. The applications presented use the recursive extended kalman filter for parameter identification which has excellent convergence for systems without process noise.

  19. Preliminary design and thermal analysis of device for finish cooling Jaffa biscuits in a.d. 'Jaffa'- Crvenka

    Directory of Open Access Journals (Sweden)

    Salemović Duško R.

    2015-01-01

    Full Text Available In this paper preliminary design of device for finish cooling chocolate topping of biscuits in A.D. 'Jaffa'- Crvenka was done. The proposed preliminary design followed by the required technological process of finish cooling biscuits and required parameters of process which was supposed to get and which represented part of project task. Thermal analysis was made and obtained percentage error between surface contact of the air and chocolate topping, obtained from heat balance and geometrical over proposed preliminary design, wasn't more than 0.67%. This is a preliminary design completely justified because using required length of belt conveyor receive required temperature of chocolate topping at the end of the cooling process.

  20. Identification of a classical mutant in the industrial host Aspergillus niger by systems genetics: LaeA is required for citric acid production and regulates the formation of some secondary metabolites

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Jing; Arentshorst, Mark; Nair, Deepa; Dai, Ziyu; Baker, Scott E.; Frisvad, Jens; Nielsen, Kristian F.; Punt, Peter J.; Ram, Arthur F.

    2016-01-11

    Rapid acidification of the culture medium by the production of organic acids and the production of acid-induced proteases are key characteristics of the filamentous fungus Aspergillus niger. The D15 mutant of A. niger is non-acidifying mutant and used often for the expression of recombinant proteins in A. niger, because of its reduced production of extracellular proteases under non-acidic conditions. In this study, the D15 mutant is characterized in detail. Strongly reduced levels of citric and oxalic acid were observed in the D15 mutant both in shake flask cultures and in controlled batch cultivations. To identify the mutation in the D15 mutant, we successfully combined high-throughput sequencing (Illumina) with bulk segregant analysis. Because of the lack of a sexual cycle for A. niger, the parasexual cycle was used to generate a pool of segregants. From the 52 single nucleotide polymorphisms (SNPs) between the parental strains, three SNPs were homozygous in the genomic DNA of pool of segregants. These three SNPs mapped to all the right arm of chromosome II, indicating that this region contains the genetic locus affecting the phenotype related to acid production. Of the three SNPs, one mutation resulted in a missense mutation in the gene encoding the A. niger homologue of the A. nidulans methyltransferase gene laeA. Complementation analysis of the original mutant with the laeA gene and targeted disruption of laeA further confirmed that LaeA is involved in citric acid production in A. niger lab (N402) and citric acid production strains (ATCC 11414). Analysis of the secondary metabolite (SM) profile of the laeA mutants indicate that LaeA is required for the production of several SMs (asperrubrol, atromentin and JBIR86), but deletion of laeA also resulted in the presence of SMs (aspernigrin A/B and BMS-192548) that were not detected in the wild-type strain. The levels of ten other SMs were not strongly affected as a result of laeA deletion indicating that only a

  1. NRT Rotor Structural / Aeroelastic Analysis for the Preliminary Design Review

    Energy Technology Data Exchange (ETDEWEB)

    Ennis, Brandon Lee [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Paquette, Joshua A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-10-01

    This document describes the initial structural design for the National Rotor Testbed blade as presented during the preliminary design review at Sandia National Laboratories on October 28- 29, 2015. The document summarizes the structural and aeroelastic requirements placed on the NRT rotor for satisfactory deployment at the DOE/SNL SWiFT experimental facility to produce high-quality datasets for wind turbine model validation. The method and result of the NRT blade structural optimization is also presented within this report, along with analysis of its satisfaction of the design requirements.

  2. Preliminary Evaluation of Removing Used Nuclear Fuel from Shutdown Sites

    Energy Technology Data Exchange (ETDEWEB)

    Maheras, Steven J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Best, Ralph E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ross, Steven B. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Buxton, Kenneth A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); England, Jeffery L. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); McConnell, Paul E. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Massaro, Lawrence M. [Federal Railroad Administration (FRA) (United States); Jensen, Philip J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2014-10-01

    This report presents a preliminary evaluation of removing used nuclear fuel (UNF) from 12 shutdown nuclear power plant sites. At these shutdown sites the nuclear power reactors have been permanently shut down and the sites have been decommissioned or are undergoing decommissioning. The shutdown sites are Maine Yankee, Yankee Rowe, Connecticut Yankee, Humboldt Bay, Big Rock Point, Rancho Seco, Trojan, La Crosse, Zion, Crystal River, Kewaunee, and San Onofre. The evaluation was divided into four components: characterization of the UNF and greater-than-Class C low-level radioactive waste (GTCC waste) inventory; a description of the on-site infrastructure and conditions relevant to transportation of UNF and GTCC waste; an evaluation of the near-site transportation infrastructure and experience relevant to shipping transportation casks containing UNF and GTCC waste, including identification of gaps in information; and, an evaluation of the actions necessary to prepare for and remove UNF and GTCC waste. The primary sources for the inventory of UNF and GTCC waste are the U.S. Department of Energy (DOE) RW-859 used nuclear fuel inventory database, industry sources such as StoreFUEL and SpentFUEL, and government sources such as the U.S. Nuclear Regulatory Commission. The primary sources for information on the conditions of site and near-site transportation infrastructure and experience included observations and information collected during visits to the Maine Yankee, Yankee Rowe, Connecticut Yankee, Humboldt Bay, Big Rock Point, Rancho Seco, Trojan, La Crosse, and Zion sites; information provided by managers at the shutdown sites; Facility Interface Data Sheets compiled for DOE in 2005; Services Planning Documents prepared for DOE in 1993 and 1994; industry publications such as Radwaste Solutions; and Google Earth. State and Regional Group representatives, a Tribal representative, and a Federal Railroad Administration representative participated in six of the shutdown site

  3. Preliminary Report on Coining of Targets

    Energy Technology Data Exchange (ETDEWEB)

    Davis, P; Wall, M; Hodge, N; Schwartz, A

    2004-07-27

    We were tasked with developing a coining technique that would evaluate the feasibility of using a pressing, or coining process to imprint a one-dimensional sinusoidal pattern onto a thin disk specimen. We performed finite element method simulations of the coining process, designed, built, and tested a coining apparatus and tested surrogate materials, and coined a sample of special nuclear material. The preliminary results were encouraging. The pressing of a 3-mm diameter by {approx}100 {micro}m thick disc to 500 pounds of pressure produced a flat part with a 1-{micro}m deep by 50-{micro}m period sine wave pattern covering all of the surface and thus demonstrated the method for replicating ultraprecision, mesoscale features onto a near-net-shape metallic blank. This coining technique is being developed to provide specialty processing for the manufacturing of difficult to machine, millimeter-size components made from materials that present hazardous conditions. The technology is versatile and can be used to imprint a wide range of features, or profiles into two opposing surfaces. The coining process requires a simple, conceivably hand held tool, which efficiently produces ultra-precision work pieces without the production of byproducts such as machining chips, or grinding swarf. It shows promise for use on ductile materials that cannot be precision machined with conventional single crystal diamond tooling. As a production process, it can be used to reduce manufacturing costs where large numbers of ultra-precision, repetitive designs are required.

  4. In-Flight System Identification

    Science.gov (United States)

    Morelli, Eugene A.

    1998-01-01

    A method is proposed and studied whereby the system identification cycle consisting of experiment design and data analysis can be repeatedly implemented aboard a test aircraft in real time. This adaptive in-flight system identification scheme has many advantages, including increased flight test efficiency, adaptability to dynamic characteristics that are imperfectly known a priori, in-flight improvement of data quality through iterative input design, and immediate feedback of the quality of flight test results. The technique uses equation error in the frequency domain with a recursive Fourier transform for the real time data analysis, and simple design methods employing square wave input forms to design the test inputs in flight. Simulation examples are used to demonstrate that the technique produces increasingly accurate model parameter estimates resulting from sequentially designed and implemented flight test maneuvers. The method has reasonable computational requirements, and could be implemented aboard an aircraft in real time.

  5. Isolation and Identification of Concrete Environment Bacteria

    Science.gov (United States)

    Irwan, J. M.; Anneza, L. H.; Othman, N.; Husnul, T.; Alshalif, A. F.

    2016-07-01

    This paper presents the isolation and molecular method for bacteria identification through PCR and DNA sequencing. Identification of the bacteria species is required in order to fully utilize the bacterium capability for precipitation of calcium carbonate in concrete. This process is to enable the addition of suitable catalyst according to the bacterium enzymatic pathway that is known through the bacteria species used. The objective of this study is to isolate, enriched and identify the bacteria species. The bacteria in this study was isolated from fresh urine and acid mine drainage water, Kota Tinggi, Johor. Enrichment of the isolated bacteria was conducted to ensure the bacteria survivability in concrete. The identification of bacteria species was done through polymerase chain reaction (PCR) and rRDNA sequencing. The isolation and enrichment of the bacteria was done successfully. Whereas, the results for bacteria identification showed that the isolated bacteria strains are Bacillus sp and Enterococus faecalis.

  6. Multifinger Feature Level Fusion Based Fingerprint Identification

    Directory of Open Access Journals (Sweden)

    Praveen N

    2012-12-01

    Full Text Available Fingerprint based authentication systems are one of the cost-effective biometric authentication techniques employed for personal identification. As the data base population increases, fast identification/recognition algorithms are required with high accuracy. Accuracy can be increased using multimodal evidences collected by multiple biometric traits. In this work, consecutive fingerprint images are taken, global singularities are located using directional field strength and their local orientation vector is formulated with respect to the base line of the finger. Featurelevel fusion is carried out and a 32 element feature template is obtained. A matching score is formulated for the identification and 100% accuracy was obtained for a database of 300 persons. The polygonal feature vector helps to reduce the size of the feature database from the present 70-100 minutiae features to just 32 features and also a lower matching threshold can be fixed compared to single finger based identification

  7. Preliminaries

    Directory of Open Access Journals (Sweden)

    Desde el jardín de Freud

    2014-10-01

    Full Text Available Desde el Jardín de Freud se encuentra indexada en el IBN-Publindex (Colciencias, en categoría C, y en Latindex. También se encuentra en las siguientes bases de datos y catálogos: ebsco, Dialnet, Rebiun, Doaj y e-Revistas.

  8. Introducing a game approach towards IS requirements specification

    DEFF Research Database (Denmark)

    Jensen, Mika Yasuoka; Kadoya, Kyoichi; Niwa, Takashi

    2014-01-01

    involvement approach with game elements. We report preliminary findings from a practice case in which our methods are applied to the requirement specification phase of a project management system. The analysis showed that our game approach fostered innovative idea generation and captured implicit user...... stakeholder involvement method with game elements can be effectively utilized as a first step towards requirement specification....

  9. Preliminary ECLSS waste water model

    Science.gov (United States)

    Carter, Donald L.; Holder, Donald W., Jr.; Alexander, Kevin; Shaw, R. G.; Hayase, John K.

    1991-01-01

    A preliminary waste water model for input to the Space Station Freedom (SSF) Environmental Control and Life Support System (ECLSS) Water Processor (WP) has been generated for design purposes. Data have been compiled from various ECLSS tests and flight sample analyses. A discussion of the characterization of the waste streams comprising the model is presented, along with a discussion of the waste water model and the rationale for the inclusion of contaminants in their respective concentrations. The major objective is to establish a methodology for the development of a waste water model and to present the current state of that model.

  10. Preliminary ECLSS waste water model

    Science.gov (United States)

    Carter, Donald L.; Holder, Donald W., Jr.; Alexander, Kevin; Shaw, R. G.; Hayase, John K.

    1991-01-01

    A preliminary waste water model for input to the Space Station Freedom (SSF) Environmental Control and Life Support System (ECLSS) Water Processor (WP) has been generated for design purposes. Data have been compiled from various ECLSS tests and flight sample analyses. A discussion of the characterization of the waste streams comprising the model is presented, along with a discussion of the waste water model and the rationale for the inclusion of contaminants in their respective concentrations. The major objective is to establish a methodology for the development of a waste water model and to present the current state of that model.

  11. Preliminary considerations concerning actinide solubilities

    Energy Technology Data Exchange (ETDEWEB)

    Newton, T.W.; Bayhurst, B.P.; Daniels, W.R.; Erdal, B.R.; Ogard, A.E.

    1980-01-01

    Work at the Los Alamos Scientific Laboratory on the fundamental solution chemistry of the actinides has thus far been confined to preliminary considerations of the problems involved in developing an understanding of the precipitation and dissolution behavior of actinide compounds under environmental conditions. Attempts have been made to calculate solubility as a function of Eh and pH using the appropriate thermodynamic data; results have been presented in terms of contour maps showing lines of constant solubility as a function of Eh and pH. Possible methods of control of the redox potential of rock-groundwater systems by the use of Eh buffers (redox couples) is presented.

  12. NSLS-II Preliminary Design Report

    Energy Technology Data Exchange (ETDEWEB)

    Dierker, S.

    2007-11-01

    Following the CD0 approval of the National Synchrotron Light Source II (NSLS-II) during August 2005, Brookhaven National Laboratory prepared a conceptual design for a worldclass user facility for scientific research using synchrotron radiation. DOE SC review of the preliminary baseline in December 2006 led to the subsequent CD1 approval (approval of alternative selection and cost range). This report is the documentation of the preliminary design work for the NSLS-II facility. The preliminary design of the Accelerator Systems (Part 1) was developed mostly based of the Conceptual Design Report, except for the Booster design, which was changed from in-storage-ring tunnel configuration to in external- tunnel configuration. The design of beamlines (Part 2) is based on designs developed by engineering firms in accordance with the specification provided by the Project. The conventional facility design (Part 3) is the Title 1 preliminary design by the AE firm that met the NSLS-II requirements. Last and very important, Part 4 documents the ES&H design and considerations related to this preliminary design. The NSLS-II performance goals are motivated by the recognition that major advances in many important technology problems will require scientific breakthroughs in developing new materials with advanced properties. Achieving this will require the development of new tools that will enable the characterization of the atomic and electronic structure, chemical composition, and magnetic properties of materials, at nanoscale resolution. These tools must be nondestructive, to image and characterize buried structures and interfaces, and they must operate in a wide range of temperatures and harsh environments. The NSLS-II facility will provide ultra high brightness and flux and exceptional beam stability. It will also provide advanced insertion devices, optics, detectors, and robotics, and a suite of scientific instruments designed to maximize the scientific output of the facility

  13. NSLS-II Preliminary Design Report

    Energy Technology Data Exchange (ETDEWEB)

    Dierker, S.

    2007-11-01

    Following the CD0 approval of the National Synchrotron Light Source II (NSLS-II) during August 2005, Brookhaven National Laboratory prepared a conceptual design for a worldclass user facility for scientific research using synchrotron radiation. DOE SC review of the preliminary baseline in December 2006 led to the subsequent CD1 approval (approval of alternative selection and cost range). This report is the documentation of the preliminary design work for the NSLS-II facility. The preliminary design of the Accelerator Systems (Part 1) was developed mostly based of the Conceptual Design Report, except for the Booster design, which was changed from in-storage-ring tunnel configuration to in external- tunnel configuration. The design of beamlines (Part 2) is based on designs developed by engineering firms in accordance with the specification provided by the Project. The conventional facility design (Part 3) is the Title 1 preliminary design by the AE firm that met the NSLS-II requirements. Last and very important, Part 4 documents the ES&H design and considerations related to this preliminary design. The NSLS-II performance goals are motivated by the recognition that major advances in many important technology problems will require scientific breakthroughs in developing new materials with advanced properties. Achieving this will require the development of new tools that will enable the characterization of the atomic and electronic structure, chemical composition, and magnetic properties of materials, at nanoscale resolution. These tools must be nondestructive, to image and characterize buried structures and interfaces, and they must operate in a wide range of temperatures and harsh environments. The NSLS-II facility will provide ultra high brightness and flux and exceptional beam stability. It will also provide advanced insertion devices, optics, detectors, and robotics, and a suite of scientific instruments designed to maximize the scientific output of the facility

  14. Preliminary design of the Space Station internal thermal control system

    Science.gov (United States)

    Herrin, Mark T.; Patterson, David W.; Turner, Larry D.

    1987-01-01

    The baseline preliminary design configuration of the Internal Thermal Control system (ITCS) of the U.S. Space Station pressurized elements (i.e., the Habitation and U.S. Laboratory modules, pressurized logistics carrier, and resources nodes) is defined. The ITCS is composed of both active and passive components. The subsystems which comprise the ITCS are identified and their functional descriptions are provided. The significant trades and analyses, which were performed during Phase B (i.e., the preliminary design phase) that resulted in the design described herein, are discussed. The ITCS interfaces with the station's central Heat Rejection and Transport System (HRTS), other systems, and externally attached pressurized payloads are described. Requirements on the ITCS with regard to redundancy and experiment support are also addressed.

  15. Preliminary functional assessment of a multigrasp myoelectric prosthesis.

    Science.gov (United States)

    Dalley, Skyler A; Bennett, Daniel A; Goldfarb, Michael

    2012-01-01

    The authors have previously described a multigrasp hand prosthesis prototype, and a two-site surface EMG based multigrasp control interface for its control. In this paper, the authors present a preliminary assessment of the efficacy of the prosthesis and multigrasp controller in performing tasks requiring interaction and manipulation. The authors use as a performance measure the Southampton Hand Assessment Procedure (SHAP), which entails manipulation of various objects designed to emulate activities of daily living, and provides a set of scores that indicate level of functionality in various types of hand function. In this preliminary assessment, a single non-amputee subject performed the SHAP while wearing the multigrasp prosthesis via an able-bodied adaptor. The results from this testing are presented, and compared to recently published SHAP results obtained with commercially available single-grasp and multigrasp prosthetic hands.

  16. MRNA-based skin identification for forensic applications

    NARCIS (Netherlands)

    M. Visser (Mijke); D. Zubakov (Dmitry); K. Ballantyne (Kaye); M.H. Kayser (Manfred)

    2011-01-01

    textabstractAlthough the identification of human skin cells is of important relevance in many forensic cases, there is currently no reliable method available. Here, we present a highly specific and sensitive messenger RNA (mRNA) approach for skin identification, meeting the key requirements in

  17. 77 FR 59575 - Hull Identification Numbers for Recreational Vessels

    Science.gov (United States)

    2012-09-28

    ... SECURITY Coast Guard 33 CFR Part 181 Hull Identification Numbers for Recreational Vessels AGENCY: Coast... requesting public comments regarding the existing regulatory requirement to indicate a boat's model year as part of the 12-character Hull Identification Number (HIN). Under current regulations in 33 CFR part 181...

  18. 33 CFR 181.29 - Hull identification number display.

    Science.gov (United States)

    2010-07-01

    ... SECURITY (CONTINUED) BOATING SAFETY MANUFACTURER REQUIREMENTS Identification of Boats § 181.29 Hull... boat hull. (a) The primary hull identification number must be affixed— (1) On boats with transoms, to... hull/deck joint, whichever is lowest. (2) On boats without transoms or on boats on which it would be...

  19. Identification of Yeasts Present in Sour Fermented Foods and Fodder

    NARCIS (Netherlands)

    Middelhoven, W.J.

    2002-01-01

    This paper deals with rapid methods for identification of 50 yeast species frequently isolated from foods and fodders that underwent a lactic acid fermentation. However, many yeast species present in olive brine, alpechin, and other olive products were not treated. The methods required for identific

  20. 9 CFR 114.4 - Identification of biological products.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Identification of biological products... REQUIREMENTS FOR BIOLOGICAL PRODUCTS § 114.4 Identification of biological products. Suitable tags or labels of... biological products, all component parts to be combined to form a biological product, all......

  1. 40 CFR 156.140 - Identification of container types.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Identification of container types. 156... PROGRAMS LABELING REQUIREMENTS FOR PESTICIDES AND DEVICES Container Labeling § 156.140 Identification of container types. For products other than plant-incorporated protectants, the following statements,...

  2. System identification of the Large-Angle Magnetic Suspension Test Fixture (LAMSTF)

    Science.gov (United States)

    Huang, Jen-Kuang

    1994-01-01

    The Large-Angle Magnetic Suspension Test Fixture (LAMSTF), a laboratory-scale research project to demonstrate the magnetic suspension of objects over wide ranges of attitudes, has been developed. This system represents a scaled model of a planned Large-Gap Magnetic Suspension System (LGMSS). The LAMSTF consists of a small cylindrical permanent magnet suspended element which is levitated above a planar array of five electromagnets mounted in a circular configuration. The cylinder is a rigid body and can be controlled to move in five independent degrees of freedom. Six position variables are sensed indirectly by using infrared light-emitting diodes and light-receiving phototransistors. The motion of the suspended cylinder is in general nonlinear and hence only the linear, time-invariant perturbed motion about an equilibrium state is considered. One of the main challenges in this project is the control of the suspended element over a wide range of orientations. An accurate dynamic model plays an essential role in controller design. The analytical model is first derived and open-loop characteristics discussed. The system is shown to be highly unstable and requires feedback control for system identification. Projection filters are first proposed to identify the state space model from closed-loop input/output test data in the time domain. This method is then extended to identify linear systems from the frequency test data. A canonical transformation matrix is also derived to transform the identified state space model into the physical coordinate. The LAMSTF system is stabilized by using a linear quadratic regulator (LQR) feedback controller for closed-loop identification. The rate information is obtained by calculating the back difference of the sensed position signals. Only the closed-loop random input/output data are recorded. Preliminary results from numerical simulations demonstrate that the identified system model is fairly accurate from either time domain or

  3. Preliminary Investigation of a Paraglider

    Science.gov (United States)

    Rogallo, Francis M.; Lowry, John G.; Croom, Delwin R.; Taylor, Robert T.

    1960-01-01

    A preliminary investigation of the aerodynamic and control characteristics of a flexible glider similar to a parachute in construction has been made at the Langley Research Center to evaluate its capabilities as a reentry glider. Preliminary weight estimates of the proposed vehicle indicate that such a structure can be made with extremely low wing loading. Maximum temperatures during the reentry maneuver might be held as low as about 1,500 F. The results of wind-tunnel and free-glide tests show that the glider when constructed of nonporous material performed extremely well at subsonic speeds and could be flown at angles of attack from about 200 to 900. At supersonic speeds the wing showed none of the unfavorable tendencies exhibited by conventional parachutes at these speeds, such as squidding and breathing. Several methods of packing and deploying the glider have been successfully demonstrated. The results of this study indicate that this flexible-lifting-surface concept may provide a lightweight controllable paraglider for manned space vehicles.

  4. Preliminary verification of structure design for CN HCCB TBM with 1 × 4 configuration

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Zhou, E-mail: zhaozhou@swip.ac.cn; Zhou, Bing; Wang, Qijie; Cao, Qixiang; Feng, Kaiming; Wang, Xiaoyu; Zhang, Guoshu

    2016-02-15

    Highlights: • A new and simplification structural design scheme with 1 × 4 configuration is proposed for CN HCCB TBM. • The detail conceptual structural design for 1 × 4 TBM is completed. • The preliminary hydraulic analysis, thermo-hydraulic analysis and structural analysis for 1 × 4 TBM had been carried out. - Abstract: Based on the conceptual design of CN HCCB TBM with 1 × 4 configuration, the preliminary hydraulic analysis, thermo-hydraulic analysis and structural analysis had been carried out for it. Hydraulic and thermo-hydraulic analyses show that the coolant manifold system could meet the fluid design requirement preliminarily and the temperature of RAFMs structural parts, Be and Li{sub 4}SiO{sub 4} pebble beds are within the allowable range, and no zone shows a stress higher than the allowable limit in the preliminary structural analysis. These results indicate the design for CN HCCB TBM with 1 × 4 configuration is preliminary reasonable.

  5. An Analysis of State Autism Educational Assessment Practices and Requirements

    Science.gov (United States)

    Barton, Erin E.; Harris, Bryn; Leech, Nancy; Stiff, Lillian; Choi, Gounah; Joel, Tiffany

    2016-01-01

    States differ in the procedures and criteria used to identify ASD. These differences are likely to impact the prevalence and age of identification for children with ASD. The purpose of the current study was to examine the specific state variations in ASD identification and eligibility criteria requirements. We examined variations by state in…

  6. An Analysis of State Autism Educational Assessment Practices and Requirements

    Science.gov (United States)

    Barton, Erin E.; Harris, Bryn; Leech, Nancy; Stiff, Lillian; Choi, Gounah; Joel, Tiffany

    2016-01-01

    States differ in the procedures and criteria used to identify ASD. These differences are likely to impact the prevalence and age of identification for children with ASD. The purpose of the current study was to examine the specific state variations in ASD identification and eligibility criteria requirements. We examined variations by state in…

  7. 6 CFR 37.13 - Document verification requirements.

    Science.gov (United States)

    2010-01-01

    ... in the situation, the State must not issue a REAL ID driver's license or identification card to the... Section 37.13 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements §...

  8. Quantum System Identification

    OpenAIRE

    Burgarth, Daniel; Yuasa, Kazuya

    2011-01-01

    The aim of quantum system identification is to estimate the ingredients inside a black box, in which some quantum-mechanical unitary process takes place, by just looking at its input-output behavior. Here we establish a basic and general framework for quantum system identification, that allows us to classify how much knowledge about the quantum system is attainable, in principle, from a given experimental setup. Prior knowledge on some elements of the black box helps the system identification...

  9. Improved Palmprint Identification System

    OpenAIRE

    Harshala C. Salave; Dr. Sachin D. Pable

    2015-01-01

    Abstract Generally private information is provided by using passwords or Personal Identification Numbers which is easy to implement but it is very easily stolen or forgotten or hack. In Biometrics for individuals identification uses human physiological which are constant throughout life like palm face DNA iris etc. or behavioral characteristicswhich is not constant in life like voice signature keystroke etc.. But mostly gain more attention to palmprint identification and is becoming more popu...

  10. Personal Identification by Eyes

    OpenAIRE

    Marinović, Dunja; Čoklo, Miran; Njirić, Sanja; Mužić, Vedrana

    2011-01-01

    Identification of persons through the eyes is in the field of biometrical science. Many security systems are based on biometric methods of personal identification, to determine whether a person is presenting itself truly. The human eye contains an extremely large number of individual characteristics that make it particularly suitable for the process of identifying a person. Today, the eye is considered to be one of the most reliable body parts for human identification. Systems usi...

  11. Preliminary design study of the TMT Telescope structure system: overview

    Science.gov (United States)

    Usuda, Tomonori; Ezaki, Yutaka; Kawaguchi, Noboru; Nagae, Kazuhiro; Kato, Atsushi; Takaki, Junji; Hirano, Masaki; Hattori, Tomoya; Tabata, Masaki; Horiuchi, Yasushi; Saruta, Yusuke; Sofuku, Satoru; Itoh, Noboru; Oshima, Takeharu; Takanezawa, Takashi; Endo, Makoto; Inatani, Junji; Iye, Masanori; Sadjadpour, Amir; Sirota, Mark; Roberts, Scott; Stepp, Larry

    2014-07-01

    We present an overview of the preliminary design of the Telescope Structure System (STR) of Thirty Meter Telescope (TMT). NAOJ was given responsibility for the TMT STR in early 2012 and engaged Mitsubishi Electric Corporation (MELCO) to take over the preliminary design work. MELCO performed a comprehensive preliminary design study in 2012 and 2013 and the design successfully passed its Preliminary Design Review (PDR) in November 2013 and April 2014. Design optimizations were pursued to better meet the design requirements and improvements were made in the designs of many of the telescope subsystems as follows: 1. 6-legged Top End configuration to support secondary mirror (M2) in order to reduce deformation of the Top End and to keep the same 4% blockage of the full aperture as the previous STR design. 2. "Double Lower Tube" of the elevation (EL) structure to reduce the required stroke of the primary mirror (M1) actuators to compensate the primary mirror cell (M1 Cell) deformation caused during the EL angle change in accordance with the requirements. 3. M1 Segment Handling System (SHS) to be able to make removing and installing 10 Mirror Segment Assemblies per day safely and with ease over M1 area where access of personnel is extremely difficult. This requires semi-automatic sequence operation and a robotic Segment Lifting Fixture (SLF) designed based on the Compliance Control System, developed for controlling industrial robots, with a mechanism to enable precise control within the six degrees of freedom of position control. 4. CO2 snow cleaning system to clean M1 every few weeks that is similar to the mechanical system that has been used at Subaru Telescope. 5. Seismic isolation and restraint systems with respect to safety; the maximum acceleration allowed for M1, M2, tertiary mirror (M3), LGSF, and science instruments in 1,000 year return period earthquakes are defined in the requirements. The Seismic requirements apply to any EL angle, regardless of the

  12. Preliminary Analysis of Remote Monitoring & Robotic Concepts for Performance Confirmation

    Energy Technology Data Exchange (ETDEWEB)

    D.A. McAffee

    1997-02-18

    ) Identify and discuss the main Performance Confirmation monitoring needs and requirements during the post-emplacement preclosure period. This includes radiological, non-radiological, host rock, and infrastructure performance monitoring needs. It also includes monitoring for possible off-normal events. (Presented in Section 7.3). (3) Identify general approaches and methods for obtaining performance information from within the emplacement drifts for Performance Confirmation. (Presented in Section 7.4) (4)Review and discuss available technologies and design strategies that may permit the use of remotely operated systems within the hostile thermal and radiation environment expected within the emplacement drifts. (Presented in Section 7.5). (5) Based on Performance Confirmation monitoring needs and available technologies, identify potential application areas for remote systems and robotics for post-emplacement preclosure Performance Confirmation activities (Presented in Section 7.6). (6) Develop preliminary remote monitoring and robotic concepts for post-emplacement, preclosure Performance Confirmation activities. (Presented in Section 7.7) This analysis is being performed very early in the systems engineering cycle, even as issues related to the Performance Confirmation program planning phase are being formulated and while the associated needs, constraints and objectives are yet to be fully determined and defined. This analysis is part of an issue formulation effort and is primarily concerned with identification and description of key issues related to remotely monitoring repository performance for Performance Confirmation. One of the purposes of this analysis is to provide an early investigation of potential design challenges that may have a high impact on future design concepts. This analysis can be used to guide future concept development and help access what is feasible and achievable by application of remote systems technology. Future design and systems engineering

  13. Facial image identification using Photomodeler.

    Science.gov (United States)

    Lynnerup, Niels; Andersen, Marie; Lauritsen, Helle Petri

    2003-09-01

    We present the results of a preliminary study on the use of 3-D software (Photomodeler) for identification purposes. Perpetrators may be photographed or filmed by surveillance systems. The police may wish to have these images compared to photographs of suspects. The surveillance imagery will often consist of many images of the same person taken from different angles. We wanted to see if it was possible to combine such a suite of images in useful 3-D renderings of facial proportions.Fifteen male adults were photographed from four different angles. Based on these photographs, a 3-D wireframe model was produced by Photomodeler. The wireframe models were then rotated to full lateral and frontal views, and compared to like sets of photographs of the subjects. In blind trials, 9/15 of the wireframe models were assigned to the correct sets of photographs. In five/15 cases, the wireframe models were assigned to several sets, including the correct set. Only in one case was a wireframe model not assigned to a correct set of photographs at all.

  14. Next Generation Microbiology Requirements

    Science.gov (United States)

    Ott, C. M.; Oubre, C. M.; Elliott, T. F.; Castro, V. A.; Pierson, D. L.

    2012-01-01

    As humans continue to explore deep into space, microorganisms will travel with them. The primary means to mitigate the risk of infectious disease are a combination of prudent spacecraft design and rigorous operational controls. The effectiveness of these methods are evaluated by microbiological monitoring of spacecraft, food, water, and the crew that is performed preflight, in-flight, and post-flight. Current NASA requirements associated with microbiological monitoring are based on culture-based methodology where microorganisms are grown on a semi-solid growth medium and enumerated. Subsequent identification of the organisms requires specialized labor and large equipment, which historically has been performed on Earth. Requirements that rely strictly on culture-based units limit the use of non-culture based monitoring technology. Specifically, the culture-based "measurement criteria" are Colony Forming Units (CFU, representing the growth of one microorganism at a single location on the agar medium) per a given volume, area, or sample size. As the CFU unit by definition is culture-based, these requirements limit alternative technologies for spaceflight applications. As spaceflight missions such as those to Mars extend further into space, culture-based technology will become difficult to implement due to the (a) limited shelf life of the culture media, (b) mass/volume necessary to carry these consumables, and (c) problems associated with the production of biohazardous material in the habitable volume of the spacecraft. In addition, an extensive amount of new knowledge has been obtained during the Space Shuttle, NASA-Mir, and International Space Station Programs, which gave direction for new or modified microbial control requirements for vehicle design and mission operations. The goal of this task is to develop and recommend a new set of requirements for vehicle design and mission operations, including microbiological monitoring, based upon "lessons learned" and new

  15. The role of Vehicles’ Identification Techniques in transportation planning – The Qatari case study

    Directory of Open Access Journals (Sweden)

    Wissam El Hamra

    2011-12-01

    Finally, and based on the comprehensive literature review undertaken in this research, in addition to the high accuracy and efficiency proven by the developed real-time modeling tools, the research recommends the implementation of Vehicles Identification Techniques in all countries that have a preliminary ITS infrastructure.

  16. Unique device identification system. Final rule.

    Science.gov (United States)

    2013-09-24

    The Food and Drug Administration (FDA) is issuing a final rule to establish a system to adequately identify devices through distribution and use. This rule requires the label of medical devices to include a unique device identifier (UDI), except where the rule provides for an exception or alternative placement. The labeler must submit product information concerning devices to FDA's Global Unique Device Identification Database (GUDID), unless subject to an exception or alternative. The system established by this rule requires the label and device package of each medical device to include a UDI and requires that each UDI be provided in a plain-text version and in a form that uses automatic identification and data capture (AIDC) technology. The UDI will be required to be directly marked on the device itself if the device is intended to be used more than once and intended to be reprocessed before each use.

  17. Sparse Matrix for ECG Identification with Two-Lead Features

    Directory of Open Access Journals (Sweden)

    Kuo-Kun Tseng

    2015-01-01

    Full Text Available Electrocardiograph (ECG human identification has the potential to improve biometric security. However, improvements in ECG identification and feature extraction are required. Previous work has focused on single lead ECG signals. Our work proposes a new algorithm for human identification by mapping two-lead ECG signals onto a two-dimensional matrix then employing a sparse matrix method to process the matrix. And that is the first application of sparse matrix techniques for ECG identification. Moreover, the results of our experiments demonstrate the benefits of our approach over existing methods.

  18. On the time required for identification of visual objects

    DEFF Research Database (Denmark)

    Petersen, Anders

    of experiments with the help of the theory of visual attention – have proven to be an effective approach to examine cognitive parameters that are essential for a broad range of different patient groups. The theory of visual attention relies on a psychometric function that describes the ability to identify...... a stimulus as a function of exposure duration. An important contribution of the thesis is that it investigates whether other psychometric functions than the one originally used with the theory of visual attention could be more appropriate at describing data. The thesis points to two psychometric functions......, with the dataset that we collected, to directly analyse how confusability develops as a certain letter is exposed for increasingly longer time. An important scientific question is what shapes the psychometric function. It is conceivable that the function reflects both limitations and structure of the physical...

  19. 49 CFR 574.5 - Tire identification requirements.

    Science.gov (United States)

    2010-10-01

    ... use with that non-pneumatic tire in S4.4 of Standard No. 129 (49 CFR 571.129). (a) First grouping. The... purchasers, or non-pneumatic tires or non-pneumatic tire assemblies, by permanently molding into or onto the... manufacturer of a non-pneumatic tire or a non-pneumatic tire assembly shall permanently mold, stamp...

  20. Anti-Exposure Technology Identification for Mission Specific Operational Requirements

    Science.gov (United States)

    1981-08-08

    underwear. Fire pit testing of this coverall for a 3-second exposure to an Av Gas fire was previously conducted (reference 10) with the following results...of the CWU-33/P Anti-Exposure Assembly did not support flame or burn through when passed through the flames of an Av Gas fire in a 25-foot path for 3

  1. Bioinformatics strategies for disease gene identification

    NARCIS (Netherlands)

    Driel, M.A. van

    2005-01-01

    Disease gene identification based on chromosomal localisation is sometimes difficult and often time-consuming. It requires collecting as much information on the disease as possible. Combining positional information with disease characteristics might give hints by which candidate disease genes can be

  2. Talent Identification and Development in Sport

    OpenAIRE

    Abbott, Angela Julia

    2006-01-01

    The early identification of talented individuals is considered increasingly important across many performance domains. Traditional concepts of talent have primarily emphasized genetically driven variables, proclaiming that exceptional abilities are the result of favourable genes matched to the required performance domain. Consequently, an oversimplified concept of sporting talent exists where the focus has typically been on discrete, one-dimensional measures at unstable periods...

  3. 23 CFR 645.109 - Preliminary engineering.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Preliminary engineering. 645.109 Section 645.109 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS UTILITIES Utility Relocations, Adjustments, and Reimbursement § 645.109 Preliminary engineering. (a) As...

  4. 33 CFR 116.10 - Preliminary review.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Preliminary review. 116.10... ALTERATION OF UNREASONABLY OBSTRUCTIVE BRIDGES § 116.10 Preliminary review. (a) Upon receipt of a written complaint, the District Commander will review the complaint to determine if, in the District...

  5. Plutonium Immobilization Can Loading Preliminary Specifications

    Energy Technology Data Exchange (ETDEWEB)

    Kriikku, E.

    1998-11-25

    This report discusses the Plutonium Immobilization can loading preliminary equipment specifications and includes a process block diagram, process description, equipment list, preliminary equipment specifications, plan and elevation sketches, and some commercial catalogs. This report identifies loading pucks into cans and backfilling cans with helium as the top priority can loading development areas.

  6. 18 CFR 1b.6 - Preliminary investigations.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Preliminary investigations. 1b.6 Section 1b.6 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.6 Preliminary investigations....

  7. Rapid identification of Streptomyces isolates by MALDI-TOF MS.

    Science.gov (United States)

    Loucif, Lotfi; Bendjama, Esma; Gacemi-Kirane, Djamila; Rolain, Jean-Marc

    2014-12-01

    The recent emergence of multidrug-resistant bacteria over the last decade has led to a renewal in the discovery of new antimicrobial drugs. Streptomyces members are practically unlimited sources of new antibiotics. However, the identification of Streptomyces species is difficult and time-consuming. Therefore, there is a need for alternative methods for their rapid identification. In this study, an efficient protocol of identification using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) was developed and applied for the rapid identification of Streptomyces isolates from the El Kala lakes in northeastern Algeria. A collection of 48 Streptomyces isolates were used for this study. The optimized procedure allowed us to obtain specific and reproducible protein spectra for each Streptomyces isolate tested. The spectra generated were used to build a preliminary local database based on their initial 16S rRNA identification. The blind test used for the identification of 20 Streptomyces strains already available in our created database and 20 unknown Streptomyces isolates showed that all (100%) of the Streptomyces strains listed in the database were rapidly (Streptomyces isolates.

  8. Health, safety and environmental requirements for composite materials

    Science.gov (United States)

    Hazer, Kathleen A.

    1994-01-01

    The health, safety and environmental requirements for the production of composite materials are discussed. The areas covered include: (1) chemical identification for each chemical; (2) toxicology; (3) industrial hygiene; (4) fire and safety; (5) environmental aspects; and (6) medical concerns.

  9. Development of a novel, simple and rapid molecular identification system for clinical Candida species

    NARCIS (Netherlands)

    Deak, R.; Bodia, L.; Aarts, H.J.M.; Maraz, A.

    2004-01-01

    Identification of clinical yeast isolates causing candidiasis is routinely performed by commercial yeast identification systems based on biochemical, morphological and physiological tests. These systems require 3-5 days and the proportion of identifications that are incorrect is high. Our novel and

  10. 75 FR 10217 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Science.gov (United States)

    2010-03-05

    ...-XU40 Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling, Release... Atlantic Shark Identification Workshops and Protected Species Safe Handling, Release, and Identification Workshops to be held in April, May, and June of 2010. Certain fishermen and shark dealers are required...

  11. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Science.gov (United States)

    2010-01-01

    ... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a) State... time a REAL ID driver's license or identification card is reissued, to ensure that the...

  12. Equipment Operational Requirements

    Energy Technology Data Exchange (ETDEWEB)

    Greenwalt, B; Henderer, B; Hibbard, W; Mercer, M

    2009-06-11

    The Iraq Department of Border Enforcement is rich in personnel, but poor in equipment. An effective border control system must include detection, discrimination, decision, tracking and interdiction, capture, identification, and disposition. An equipment solution that addresses only a part of this will not succeed, likewise equipment by itself is not the answer without considering the personnel and how they would employ the equipment. The solution should take advantage of the existing in-place system and address all of the critical functions. The solutions are envisioned as being implemented in a phased manner, where Solution 1 is followed by Solution 2 and eventually by Solution 3. This allows adequate time for training and gaining operational experience for successively more complex equipment. Detailed descriptions of the components follow the solution descriptions. Solution 1 - This solution is based on changes to CONOPs, and does not have a technology component. It consists of observers at the forts and annexes, forward patrols along the swamp edge, in depth patrols approximately 10 kilometers inland from the swamp, and checkpoints on major roads. Solution 2 - This solution adds a ground sensor array to the Solution 1 system. Solution 3 - This solution is based around installing a radar/video camera system on each fort. It employs the CONOPS from Solution 1, but uses minimal ground sensors deployed only in areas with poor radar/video camera coverage (such as canals and streams shielded by vegetation), or by roads covered by radar but outside the range of the radar associated cameras. This document provides broad operational requirements for major equipment components along with sufficient operational details to allow the technical community to identify potential hardware candidates. Continuing analysis will develop quantities required and more detailed tactics, techniques, and procedures.

  13. Space power development impact on technology requirements

    Science.gov (United States)

    Cassidy, J. F.; Fitzgerald, T. J.; Gilje, R. I.; Gordon, J. D.

    1986-01-01

    The paper is concerned with the selection of a specific spacecraft power technology and the identification of technology development to meet system requirements. Requirements which influence the selection of a given technology include the power level required, whether the load is constant or transient in nature, and in the case of transient loads, the time required to recover the power, and overall system safety. Various power technologies, such as solar voltaic power, solar dynamic power, nuclear power systems, and electrochemical energy storage, are briefly described.

  14. Development and preliminary evaluation of a music-based attention assessment for patients with traumatic brain injury.

    Science.gov (United States)

    Jeong, Eunju; Lesiuk, Teresa L

    2011-01-01

    Impairments in attention are commonly seen in individuals with traumatic brain injury (TBI). While visual attention assessment measurements have been rigorously developed and frequently used in cognitive neurorehabilitation, there is a paucity of auditory attention assessment measurements for patients with TBI. The purpose of this study was to field test a researcher-developed Music-based Attention Assessment (MAA), a melodic contour identification test designed to assess three different types of attention (i.e., sustained attention, selective attention, and divided attention), for patients with TBI. Additionally, this study aimed to evaluate the readability and comprehensibility of the test items and to examine the preliminary psychometric properties of the scale and test items. Fifteen patients diagnosed with TBI completed 3 different series of tasks in which they were required to identify melodic contours. The resulting data showed that (a) test items in each of the 3 subtests were found to have an easy to moderate level of item difficulty and an acceptable to high level of item discrimination, and (b) the musical characteristics (i.e., contour, congruence, and pitch interference) were found to be associated with the level of item difficulty, and (c) the internal consistency of the MAA as computed by Cronbach's alpha was .95. Subsequent studies using a larger sample of typical participants, along with individuals with TBI, are needed to confirm construct validity and internal consistency of the MAA. In addition, the authors recommend examination of criterion validity of the MAA as correlated with current neuropsychological attention assessment measurements.

  15. Remote Spectroscopic Identification of Bloodstains

    NARCIS (Netherlands)

    R.H. Bremmer; G. Edelman; T. Dijn Vegter; T. Bijvoets; M.C.G. Aalders

    2011-01-01

    Blood detection and identification at crime scenes are crucial for harvesting forensic evidence. Unfortunately, most tests for the identification of blood are destructive and time consuming. We present a fast and nondestructive identification test for blood, using noncontact reflectance spectroscopy

  16. Control Oriented System Identification

    Science.gov (United States)

    1993-08-01

    The research goals for this grant were to obtain algorithms for control oriented system identification is to construct dynamical models of systems...and measured information. Algorithms for this type of nonlinear system identification have been given that produce models suitable for gain scheduled

  17. Multicriteria identification sets method

    Science.gov (United States)

    Kamenev, G. K.

    2016-11-01

    A multicriteria identification and prediction method for mathematical models of simulation type in the case of several identification criteria (error functions) is proposed. The necessity of the multicriteria formulation arises, for example, when one needs to take into account errors of completely different origins (not reducible to a single characteristic) or when there is no information on the class of noise in the data to be analyzed. An identification sets method is described based on the approximation and visualization of the multidimensional graph of the identification error function and sets of suboptimal parameters. This method allows for additional advantages of the multicriteria approach, namely, the construction and visual analysis of the frontier and the effective identification set (frontier and the Pareto set for identification criteria), various representations of the sets of Pareto effective and subeffective parameter combinations, and the corresponding predictive trajectory tubes. The approximation is based on the deep holes method, which yields metric ɛ-coverings with nearly optimal properties, and on multiphase approximation methods for the Edgeworth-Pareto hull. The visualization relies on the approach of interactive decision maps. With the use of the multicriteria method, multiple-choice solutions of identification and prediction problems can be produced and justified by analyzing the stability of the optimal solution not only with respect to the parameters (robustness with respect to data) but also with respect to the chosen set of identification criteria (robustness with respect to the given collection of functionals).

  18. Identification of entomopathogenic fungi

    Science.gov (United States)

    This chapter provides essential assistance for the identification of the most important genera (and main species) of fungal pathogens affecting insects, mites, and spiders. The key allows identifications regardless of which major spore types might be present with the specimen. The phylogenetic affi...

  19. 7 CFR 58.530 - Keeping quality requirements.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Keeping quality requirements. 58.530 Section 58.530... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Requirements for Cottage Cheese Bearing Usda Official Identification § 58.530 Keeping quality requirements. Keeping quality...

  20. 7 CFR 58.650 - Requirements for frozen custard.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Requirements for frozen custard. 58.650 Section 58.650... Products Bearing Usda Official Identification § 58.650 Requirements for frozen custard. The same requirements apply as for ice cream except plain frozen custard shall have a minimum egg yolk solids content...

  1. The influence of and the identification of nonlinearity in flexible structures

    Science.gov (United States)

    Zavodney, Lawrence D.

    1988-01-01

    Several models were built at NASA Langley and used to demonstrate the following nonlinear behavior: internal resonance in a free response, principal parametric resonance and subcritical instability in a cantilever beam-lumped mass structure, combination resonance in a parametrically excited flexible beam, autoparametric interaction in a two-degree-of-freedom system, instability of the linear solution, saturation of the excited mode, subharmonic bifurcation, and chaotic responses. A video tape documenting these phenomena was made. An attempt to identify a simple structure consisting of two light-weight beams and two lumped masses using the Eigensystem Realization Algorithm showed the inherent difficulty of using a linear based theory to identify a particular nonlinearity. Preliminary results show the technique requires novel interpretation, and hence may not be useful for structural modes that are coupled by a guadratic nonlinearity. A literature survey was also completed on recent work in parametrically excited nonlinear system. In summary, nonlinear systems may possess unique behaviors that require nonlinear identification techniques based on an understanding of how nonlinearity affects the dynamic response of structures. In this was, the unique behaviors of nonlinear systems may be properly identified. Moreover, more accutate quantifiable estimates can be made once the qualitative model has been determined.

  2. Improved Palmprint Identification System

    Directory of Open Access Journals (Sweden)

    Harshala C. Salave

    2015-03-01

    Full Text Available Abstract Generally private information is provided by using passwords or Personal Identification Numbers which is easy to implement but it is very easily stolen or forgotten or hack. In Biometrics for individuals identification uses human physiological which are constant throughout life like palm face DNA iris etc. or behavioral characteristicswhich is not constant in life like voice signature keystroke etc.. But mostly gain more attention to palmprint identification and is becoming more popular technique using for identification and promising alternatives to the traditional password or PIN based authentication techniques. In this paper propose palmprint identification using veins on the palm and fingers. Here use fusion of techniques such as Discrete Wavelet transformDWT Canny Edge Detector Gaussian Filter Principle Component AnalysisPCA.

  3. Preliminary design report for OTEC stationkeeping subsystems (SKSS)

    Energy Technology Data Exchange (ETDEWEB)

    1979-12-12

    Lockheed Ocean Systems with IMODCO prepared these preliminary designs for OTEC Stationkeeping Subsystems (SKSS) under contract to NOAA in support of the Department of Energy OTEC program. The results of Tasks III, V, and VI are presented in this design report. The report consists of five sections: introduction, preliminary designs for the multiple anchor leg (MAL) and tension anchor leg (TAL), costs and schedule, and conclusions. Extensive appendixes provide detailed descriptions of design methodology and include backup calculations and data to support the results presented. The objective of this effort is to complete the preliminary designs for the barge-MAL and Spar-TAL SKSS. A set of drawings is provided for each which show arrangements, configuration, component details, engineering description, and deployment plan. Loads analysis, performance assessment, and sensitivity to requirements are presented, together with the methodology employed to analyze the systems and to derive the results presented. Life cycle costs and schedule are prepared and compared on a common basis. Finally, recommendations for the Commercial Plant SKSS are presented for both platform types.

  4. Preliminary siting activities for new waste handling facilities at the Idaho National Engineering Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, D.D.; Hoskinson, R.L.; Kingsford, C.O.; Ball, L.W.

    1994-09-01

    The Idaho Waste Processing Facility, the Mixed and Low-Level Waste Treatment Facility, and the Mixed and Low-Level Waste Disposal Facility are new waste treatment, storage, and disposal facilities that have been proposed at the Idaho National Engineering Laboratory (INEL). A prime consideration in planning for such facilities is the selection of a site. Since spring of 1992, waste management personnel at the INEL have been involved in activities directed to this end. These activities have resulted in the (a) identification of generic siting criteria, considered applicable to either treatment or disposal facilities for the purpose of preliminary site evaluations and comparisons, (b) selection of six candidate locations for siting,and (c) site-specific characterization of candidate sites relative to selected siting criteria. This report describes the information gathered in the above three categories for the six candidate sites. However, a single, preferred site has not yet been identified. Such a determination requires an overall, composite ranking of the candidate sites, which accounts for the fact that the sites under consideration have different advantages and disadvantages, that no single site is superior to all the others in all the siting criteria, and that the criteria should be assigned different weighing factors depending on whether a site is to host a treatment or a disposal facility. Stakeholder input should now be solicited to help guide the final selection. This input will include (a) siting issues not already identified in the siting, work to date, and (b) relative importances of the individual siting criteria. Final site selection will not be completed until stakeholder input (from the State of Idaho, regulatory agencies, the public, etc.) in the above areas has been obtained and a strategy has been developed to make a composite ranking of all candidate sites that accounts for all the siting criteria.

  5. Integration of photovoltaic units into electric utility grids: experiment information requirements and selected issues

    Energy Technology Data Exchange (ETDEWEB)

    1980-09-01

    A number of investigations have led to the recognition of technical, economic, and institutional issues relating to the interface between solar electric technologies and electric utility systems. These issues derive from three attributes of solar electric power concepts, including (1) the variability and unpredictability of the solar resources, (2) the dispersed nature of those resources which suggest the deployment of small dispersed power units, and (3) a high initial capital cost coupled with relatively low operating costs. An important part of the DOE programs to develop new source technologies, in particular photovoltaic systems, is the experimental testing of complete or nearby complete power units. These experiments provide an opportunity to examine operational and integration issues which must be understood before widespread commercial deployment of these technologies can be achieved. Experiments may also be required to explicitly examine integration, operational, and control aspects of single and multiple new source technology power units within a utility system. An identification of utility information requirements, a review of planned experiments, and a preliminary determination of additional experimental needs and opportunities are presented. Other issues discussed include: (1) the impacts of on-site photovoltaic units on load duration curves and optimal generation mixes are considered; (2) the impacts of on-site photovoltaic units on utility production costs, with and without dedicated storage and with and without sellback, are analyzed; and (3) current utility rate structure experiments, rationales, policies, practices, and plans are reviewed.

  6. Identification of yeasts present in sour fermented foods and fodders.

    Science.gov (United States)

    Middelhoven, Wouter J

    2002-07-01

    This paper deals with rapid methods for identification of 50 yeast species frequently isolated from foods and fodders that underwent a lactic acid fermentation. However, many yeast species present in olive brine, alpechin, and other olive products were not treated. The methods required for identification include light microscopy, physiological growth tests (ID32C system of BioMérieux), assimilation of nitrate and of ethylamine as sole nitrogen sources, vitamin requirement, and maximum growth temperature. An identification key to treated yeast species is provided. In another table characteristics of all yeast species treated are listed.

  7. An Analysis of State Autism Educational Assessment Practices and Requirements.

    Science.gov (United States)

    Barton, Erin E; Harris, Bryn; Leech, Nancy; Stiff, Lillian; Choi, Gounah; Joel, Tiffany

    2016-03-01

    States differ in the procedures and criteria used to identify ASD. These differences are likely to impact the prevalence and age of identification for children with ASD. The purpose of the current study was to examine the specific state variations in ASD identification and eligibility criteria requirements. We examined variations by state in autism assessment practices and the proportion of children eligible for special education services under the autism category. Overall, our findings suggest that ASD identification practices vary across states, but most states use federal guidelines, at least in part, to set their requirements. Implications and recommendations for policy and practice are discussed.

  8. Preliminary AD-Horn Thermomechanical and Electrodynamic Simulations

    CERN Document Server

    AUTHOR|(CDS)2095747; Horvath, David; Calviani, Marco

    2016-01-01

    As part of the Antiproton Decelerator (AD) target area consolidation activities planned for LS2, it has been necessary to perform a comprehensive study of the thermo-structural behaviour of the AD magnetic horn during operation, in order to detail specific requirements for the upgrade projects and testing procedures. The present work illustrates the preliminary results of the finite element analysis carried out to evaluate the thermal and structural behaviour of the device, as well as the methodology used to model and solve the thermomechanical and electrodynamic simulations performed in the AD magnetic horn.

  9. Realization and preliminary measurements on a 94 GHz SIS mixer

    CERN Document Server

    Oberto, L; Fretto, M; Tartari, A; Brunetti, L; Lacquaniti, V

    2010-01-01

    In this paper we present the realization and a preliminary characterization of a SIS based receiver. It has been developed for the MASTER experiment that consists in a three-band SIS receiver (94, 225 and 345 GHz) for astrophysical observations through the atmospheric windows available at high altitude dry sites. The measurements performed establish an upper limit to the overall receiver noise temperature. A comparison has been tried with the MASTER requirements and with state of the art results. A noise figure of 110 K has been obtained at 94 GHz, about 22 times the quantum limit.

  10. Design and preliminary testing of the RIC hybrid knee prosthesis.

    Science.gov (United States)

    Lenzi, T; Sensinger, J; Lipsey, J; Hargrove, L; Kuiken, T

    2015-08-01

    We present a novel hybrid knee prosthesis that uses a motor, transmission and control system only for active dynamics tasks, while relying on a spring/damper system for passive dynamics activities. Active dynamics tasks require higher torque, lower speed, and occur less frequently than passive dynamic activities. By designing the actuation system around active tasks alone, we achieved a lightweight design (1.7 Kg w/o battery) without sacrificing peak torque (85Nm repetitive). Preliminary tests performed by an able-bodied person using a bypass orthosis show that the hybrid knee can support reciprocal stairs ambulation with low electrical energy consumption.

  11. Preliminary Design of a LSA Aircraft Using Wind Tunnel Tests

    Directory of Open Access Journals (Sweden)

    Norbert ANGI

    2015-12-01

    Full Text Available This paper presents preliminary results concerning the design and aerodynamic calculations of a light sport aircraft (LSA. These were performed for a new lightweight, low cost, low fuel consumption and long-range aircraft. The design process was based on specific software tools as Advanced Aircraft Analysis (AAA, XFlr 5 aerodynamic and dynamic stability analysis, and Catia design, according to CS-LSA requirements. The calculations were accomplished by a series of tests performed in the wind tunnel in order to assess experimentally the aerodynamic characteristics of the airplane.

  12. Preliminary wind tunnel tests on the pedal wind turbine

    Science.gov (United States)

    Vinayagalingam, T.

    1980-06-01

    High solidity-low speed wind turbines are relatively simple to construct and can be used advantageously in many developing countries for such direct applications as water pumping. Established designs in this class, such as the Savonius and the American multiblade rotors, have the disadvantage that their moving surfaces require a rigid construction, thereby rendering large units uneconomical. In this respect, the pedal wind turbine recently reported by the author and which incorporates sail type rotors offers a number of advantages. This note reports preliminary results from a series of wind tunnel tests which were carried out to assess the aerodynamic torque and power characteristics of the turbine.

  13. Model Identification and Validation for a Heating System using MATLAB System Identification Toolbox

    Science.gov (United States)

    Junaid Rabbani, Muhammad; Hussain, Kashan; khan, Asim-ur-Rehman; Ali, Abdullah

    2013-12-01

    This paper proposed a systematic approach to select a mathematical model for an industrial heating system by adopting system identification techniques with the aim of fulfilling the design requirement for the controller. The model identification process will begin by collecting real measurement data samples with the aid of MATLAB system identification toolbox. The criteria for selecting the model that could validate model output with actual data will based upon: parametric identification technique, picking the best model structure with low order among ARX, ARMAX and BJ, and then applying model estimation and validation tests. Simulated results have shown that the BJ model has been best in providing good estimation and validation based upon performance criteria such as: final prediction error, loss function, best percentage of model fit, and co-relation analysis of residual for output.

  14. Decentralized system identification using stochastic subspace identification for wireless sensor networks.

    Science.gov (United States)

    Cho, Soojin; Park, Jong-Woong; Sim, Sung-Han

    2015-04-08

    Wireless sensor networks (WSNs) facilitate a new paradigm to structural identification and monitoring for civil infrastructure. Conventional structural monitoring systems based on wired sensors and centralized data acquisition systems are costly for installation as well as maintenance. WSNs have emerged as a technology that can overcome such difficulties, making deployment of a dense array of sensors on large civil structures both feasible and economical. However, as opposed to wired sensor networks in which centralized data acquisition and processing is common practice, WSNs require decentralized computing algorithms to reduce data transmission due to the limitation associated with wireless communication. In this paper, the stochastic subspace identification (SSI) technique is selected for system identification, and SSI-based decentralized system identification (SDSI) is proposed to be implemented in a WSN composed of Imote2 wireless sensors that measure acceleration. The SDSI is tightly scheduled in the hierarchical WSN, and its performance is experimentally verified in a laboratory test using a 5-story shear building model.

  15. Preliminary Study on the Establishment of Regulatory Infrastructure for a Commercial SMART Licensing

    Energy Technology Data Exchange (ETDEWEB)

    Yune, Young Gill; Roh, Kyung Wan; Kang, Dong Gu; Kim, Hho Jung; Jo, Jong Chull [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

    2007-10-15

    The SMART, an integral type of PWR with the capacity of 330MWth, has been developed for multi-purposes such as seawater desalination, ship propulsion, and district heating in Korea since early 1990s. The basic design of the SMART had been completed in 2002. For the demonstration of the safety and performance of the SMART, a project to construct a 1/5 scaled-down prototype reactor with capacity of 65MWth, called as SMART-P(SMART Pilot Plant), has been performed from 2002 to 2006. However, in 2006, the project to construct the SMART-P was replaced with a preliminary project to develop a commercial SMART in order to advance commercialization of the SMART. Since the SMART is the first commercial reactor adopting the integral design concept and new system/components such as passive residual heat removal system, canned motor pump, and helical tube steam generator in Korea, some verification tests need to be performed to demonstrate the safety and performance of the SMART. Also, since several new design computer codes that have not yet been confirmed by the regulatory body are expected to be used in the design, some validation tests should be performed to demonstrate their applicability for important accident phenomena. In order to carry out the SMART development project satisfactorily and to prevent any undesirable delay of licensing process, it is necessary to establish a well-planned verification test program in advance through the early identification of the scope and items of the verification tests. Also, the issues for which either policy decision-makings or detailed technical evaluations are needed should be identified in an early stage of design, and the regulatory positions for the issues should be provided either timely or in advance. Advance identification of regulatory requirements/guides to be amended or newly developed enables to provide regulatory directions for such items in the early stage of design. The objective of this paper is to introduce the current

  16. Desempenho de escolares de 1ª série na bateria de identificação de erros de reversão e inversão na escrita: estudo preliminar Performance of students from 1st grade in the inversion and reversion errors in the written identification test: preliminary study

    Directory of Open Access Journals (Sweden)

    Beatriz Aparecida Gimenez Gonçalves

    2010-12-01

    Full Text Available OBJETIVO: caracterizar o desempenho de escolares da 1ª série na bateria de identificação de erros de reversão e inversão na escrita. MÉTODOS: participaram deste estudo 30 escolares de 1ª série de ensino público do município da cidade de Marília-SP, de ambos os gêneros, na faixa etária de 7 anos a 7 anos e 11 meses de idade. Como procedimento foi realizada a adaptação brasileira da bateria de identificação dos erros de reversão e inversão na escrita. Esta bateria é composta por 3 testes: teste de desempenho contínuo, teste de memória de curto prazo e teste de controle de escrita. Cada teste é composto por subtestes que visam identificar a capacidade dos escolares em identificar as inversões e reversões de letras e números isolados e letras e números em sequências. RESULTADOS: os resultados revelaram que os escolares da 1ª série apresentaram desempenho superior para identificação de reversão e inversão de letras dentro de palavras do que isoladamente, identificação da reversão para letras e números com desempenho superior se comparado a inversão de letras na palavra. Além disso, os escolares deste estudo apresentaram menor tempo para identificação de reversão e inversão de letras e números isolados do que em sequência. CONCLUSÃO: a Bateria adaptada para este estudo permitiu conhecer o perfil dos escolares de 1ª série quanto a identificação dos erros de inversão e reversão, entretanto, é necessário novas aplicações para verificar a ocorrência de identificação destes erros em populações de diferentes fase de alfabetização.PURPOSE: to characterize the performance of students from 1st grade in the inversion and reversion errors in the written identification Brazilian Test. METHODS: 30 students from 1st grade of a public school in Marília City, from both genders, ranging from 7 to 7 years and 11 month old took part. As a procedure, we carried out the inversion and reversion errors in the

  17. Temperature Requirements of Some Common Forensically Important Blow and Flesh Flies (Diptera under Laboratory Conditions

    Directory of Open Access Journals (Sweden)

    AH Shiravi

    2011-06-01

    Conclusion: This is the first report on thermal requirement of three forensic flies in Iran. The data of this study provide preliminary information for forensic entomologist to establish PMI in the area of study.  

  18. Births: preliminary data for 2012.

    Science.gov (United States)

    Hamilton, Brady E; Martin, Joyce A; Ventura, Stephanie J

    2013-09-01

    Objectives-This report presents preliminary data for 2012 on births in the United States. U.S. data on births are shown by age, live-birth order, race, and Hispanic origin of mother. Data on marital status, cesarean delivery, preterm births, and low birthweight are also presented. Methods-Data in this report are based on 99.96% of 2012 births.Records for the few states with less than 100% of records received are weighted to independent control counts of all births received in state vital statistics offices in 2012. Comparisons are made with final 2011 data. Results-The preliminary number of births for the United States in 2012 was 3,952,937, essentially unchanged (not statistically significant) from 2011; the general fertility rate was 63.0 births per 1,000 women aged 15-44, down only slightly from 2011, after declining nearly 3% a year from 2007 through 2010. The number of births and fertility rate either declined or were unchanged for most race and Hispanic origin groups from 2011 to 2012; however, both the number of births and the fertility rate for Asian or Pacific Islander women rose in 2012 (7% and 4%, respectively). The birth rate for teenagers aged 15-19 was down 6% in 2012 (29.4 births per 1,000 teenagers aged 15-19), yet another historic low for the United States, with rates declining for younger and older teenagers and for nearly all race and Hispanic origin groups. The birth rate for women in their early 20s also declined in 2012, to a new record low of 83.1 births per 1,000 women. Birth rates for women in their 30s rose in 2012, as did the birth rate for women in their early 40s. The birth rate for women in their late 40s was unchanged. The nonmarital birth rate declined in 2012 (to 45.3 birth per 1,000 unmarried women aged 15-44), whereas the number of births to unmarried women rose 1% and the percentage of births to unmarried women was unchanged (at 40.7%). The cesarean delivery rate for the United States was unchanged in 2012 at 32.8%. The preterm

  19. Closed Loop Subspace Identification

    Directory of Open Access Journals (Sweden)

    Geir W. Nilsen

    2005-07-01

    Full Text Available A new three step closed loop subspace identifications algorithm based on an already existing algorithm and the Kalman filter properties is presented. The Kalman filter contains noise free states which implies that the states and innovation are uneorre lated. The idea is that a Kalman filter found by a good subspace identification algorithm will give an output which is sufficiently uncorrelated with the noise on the output of the actual process. Using feedback from the output of the estimated Kalman filter in the closed loop system a subspace identification algorithm can be used to estimate an unbiased model.

  20. RECURSIVE SYSTEM IDENTIFICATION

    Institute of Scientific and Technical Information of China (English)

    Han-Fu Chen

    2009-01-01

    Most of existing methods in system identification with possible exception of those for linear systems are off-line in nature, and hence are nonrecursive.This paper demonstrates the recent progress in recursive system identification.The recursive identifi-cation algorithms are presented not only for linear systems (multivariate ARMAX systems) but also for nonlinear systems such as the Hammerstein and Wiener systems, and the non-linear ARX systems.The estimates generated by the algorithms are online updated and converge a.s.to the true values as time tends to infinity.

  1. Biometrics, identification and surveillance.

    Science.gov (United States)

    Lyon, David

    2008-11-01

    Governing by identity describes the emerging regime of a globalizing, mobile world. Governance depends on identification but identification increasingly depends on biometrics. This 'solution' to difficulties of verification is described and some technical weaknesses are discussed. The role of biometrics in classification systems is also considered and is shown to contain possible prejudice in relation to racialized criteria of identity. Lastly, the culture of biometric identification is shown to be limited to abstract data, artificially separated from the lived experience of the body including the orientation to others. It is proposed that creators of national ID systems in particular address these crucial deficiencies in their attempt to provide new modes of verification.

  2. Personal identification by eyes.

    Science.gov (United States)

    Marinović, Dunja; Njirić, Sanja; Coklo, Miran; Muzić, Vedrana

    2011-09-01

    Identification of persons through the eyes is in the field of biometrical science. Many security systems are based on biometric methods of personal identification, to determine whether a person is presenting itself truly. The human eye contains an extremely large number of individual characteristics that make it particularly suitable for the process of identifying a person. Today, the eye is considered to be one of the most reliable body parts for human identification. Systems using iris recognition are among the most secure biometric systems.

  3. Identification, Classification and Zooarchaeology

    Directory of Open Access Journals (Sweden)

    Jonathan C. Driver

    2011-08-01

    Full Text Available Identification of preserved biological materials is often regarded as a skill which has little to do with analysis and interpretation. This paper argues that in zooarchaeological studies―here with particular reference to vertebrate remains―identification procedures deserve more detailed consideration, because these procedures have a significant effect on the results of faunal studies. It is suggested that most identifications are made within a system of usually unspecified rules which vary from one analyst to another. Improvements in comparability between faunal studies will result if these rules are considered before beginning an analysis, and if the rules are made explicit in publications.

  4. Supervised Classification Methods for Seismic Phase Identification

    Science.gov (United States)

    Schneider, Jeff; Given, Jeff; Le Bras, Ronan; Fisseha, Misrak

    2010-05-01

    The Comprehensive Nuclear Test Ban Treaty Organization (CTBTO) is tasked with monitoring compliance with the CTBT. The organization is installing the International Monitoring System (IMS), a global network of seismic, hydroacoustic, infrasound, and radionuclide sensor stations. The International Data Centre (IDC) receives the data from seismic stations either in real time or on request. These data are first processed on a station per station basis. This initial step yields discrete detections which are then assembled on a network basis (with the addition of hydroacoustic and infrasound data) to produce automatic and analyst reviewed bulletins containing seismic, hydroacoustic, and infrasound detections. The initial station processing step includes the identification of seismic and acoustic phases which are given a label. Subsequent network processing relies on this preliminary labeling, and as a consequence, the accuracy and reliability of automatic and reviewed bulletins also depend on this initial step. A very large ground truth database containing massive amounts of detections with analyst-reviewed labels is available to improve on the current operational system using machine learning methods. An initial study using a limited amount of data was conducted during the ISS09 project of the CTBTO. Several classification methods were tested: decision tree with bagging; logistic regression; neural networks trained with back-propagation; Bayesian networks as generative class models; naive Bayse classification; support vector machines. The initial assessment was that the phase identification process could be improved by at least 13% over the current operational system and that the method obtaining the best results was the decision tree with bagging. We present the results of a study using a much larger learning dataset and preliminary implementation results.

  5. Preliminary Iron Distribution on Vesta

    Science.gov (United States)

    Mittlefehldt, David W.; Mittlefehldt, David W.

    2013-01-01

    The distribution of iron on the surface of the asteroid Vesta was investigated using Dawn's Gamma Ray and Neutron Detector (GRaND) [1,2]. Iron varies predictably with rock type for the howardite, eucrite, and diogenite (HED) meteorites, thought to be representative of Vesta. The abundance of Fe in howardites ranges from about 12 to 15 wt.%. Basaltic eucrites have the highest abundance, whereas, lower crustal and upper mantle materials (cumulate eucrites and diogenites) have the lowest, and howardites are intermediate [3]. We have completed a mapping study of 7.6 MeV gamma rays produced by neutron capture by Fe as measured by the bismuth germanate (BGO) detector of GRaND [1]. The procedures to determine Fe counting rates are presented in detail here, along with a preliminary distribution map, constituting the necessary initial step to quantification of Fe abundances. We find that the global distribution of Fe counting rates is generally consistent with independent mineralogical and compositional inferences obtained by other instruments on Dawn such as measurements of pyroxene absorption bands by the Visual and Infrared Spectrometer (VIR) [4] and Framing Camera (FC) [5] and neutron absorption measurements by GRaND [6].

  6. EUPORIAS: plans and preliminary results

    Science.gov (United States)

    Buontempo, C.

    2013-12-01

    Recent advances in our understanding and ability to forecast climate variability have meant that skilful predictions are beginning to be routinely made on seasonal to decadal (s2d) timescales. Such forecasts have the potential to be of great value to a wide range of decision-making, where outcomes are strongly influenced by variations in the climate. In 2012 the European Commission funded EUPORIAS, a four year long project to develop prototype end-to-end climate impact prediction services operating on a seasonal to decadal timescale, and assess their value in informing decision-making. EUPORIAS commenced on 1 November 2012, coordinated by the UK Met Office leading a consortium of 24 organisations representing world-class European climate research and climate service centres, expertise in impacts assessments and seasonal predictions, two United Nations agencies, specialists in new media, and commercial companies in climate-vulnerable sectors such as energy, water and tourism. The poster describes the setup of the project, its main outcome and some of the very preliminary results.

  7. Preliminary results of ANAIS-25

    CERN Document Server

    Amaré, J; Cuesta, C; García, E; Ginestra, C; Martínez, M; Oliván, M A; Ortigoza, Y; de Solórzano, A Ortiz; Pobes, C; Puimedón, J; Sarsa, M L; Villar, P; Villar, J A

    2013-01-01

    The ANAIS (Annual Modulation with NaI(Tl) Scintillators) experiment aims at the confirmation of the DAMA/LIBRA signal using the same target and technique at the Canfranc Underground Laboratory. 250 kg of ultrapure NaI(Tl) crystals will be used as a target, divided into 20 modules, each coupled to two photomultipliers. Two NaI(Tl) crystals of 12.5 kg each, grown by Alpha Spectra from a powder having a potassium level under the limit of our analytical techniques, form the ANAIS-25 set-up. The background contributions are being carefully studied and preliminary results are presented: their natural potassium content in the bulk has been quantified, as well as the uranium and thorium radioactive chains presence in the bulk through the discrimination of the corresponding alpha events by PSA, and due to the fast commissioning, the contribution from cosmogenic activated isotopes is clearly identified and their decay observed along the first months of data taking. Following the procedures established with ANAIS-0 and ...

  8. Continuous-Time Bilinear System Identification

    Science.gov (United States)

    Juang, Jer-Nan

    2003-01-01

    The objective of this paper is to describe a new method for identification of a continuous-time multi-input and multi-output bilinear system. The approach is to make judicious use of the linear-model properties of the bilinear system when subjected to a constant input. Two steps are required in the identification process. The first step is to use a set of pulse responses resulting from a constant input of one sample period to identify the state matrix, the output matrix, and the direct transmission matrix. The second step is to use another set of pulse responses with the same constant input over multiple sample periods to identify the input matrix and the coefficient matrices associated with the coupling terms between the state and the inputs. Numerical examples are given to illustrate the concept and the computational algorithm for the identification method.

  9. A Computer Based Decision Support System for Tailoring Logistics Support Analysis Record (LSAR) Requirements

    Science.gov (United States)

    1989-09-01

    L-7190, Preliminary Maintenance Allocation Chart: The Preliminary Miantenance Allocation Chart ( PMAC ) is a list of all items, down to the lowest level...operations, and remarks required to explain the maintenance operations. The PMAC includes additional data (over and above the required MAC data) and may be...used to develop the MAC for the organizational technical manual. Use LSAR Input data records C, Dl, H, H1 to arrange the data in PMAC format and

  10. A Preliminary List of the Birds of the Karoo National Park

    Directory of Open Access Journals (Sweden)

    R. Martin

    1988-10-01

    Full Text Available A preliminary list of the birds of the Karoo National Park is presented. Details of status, habitat preference and breeding (where applicable are given. A supplementary list of birds which require confirmation or which were only recorded in the park before the study period, is included.

  11. ANSI/ASHRAE/IES Standard 90.1-2013 Preliminary Determination: Quantitative Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Halverson, Mark A.; Rosenberg, Michael I.; Wang, Weimin; Zhang, Jian; Mendon, Vrushali V.; Athalye, Rahul A.; Xie, YuLong; Hart, Reid; Goel, Supriya

    2014-03-01

    This report provides a preliminary quantitative analysis to assess whether buildings constructed according to the requirements of ANSI/ASHRAE/IES Standard 90.1-2013 would result in energy savings compared with buildings constructed to ANSI/ASHRAE/IES Standard 90.1-2010.

  12. Screening and Identification a Serratia marcescen Strain Producing Red-Pigment and Preliminary Study of the Fermentation Conditions%一株产灵菌红素粘质沙雷氏菌的筛选、鉴定及发酵条件

    Institute of Scientific and Technical Information of China (English)

    李子武; 张显; 徐美娟; 夏海锋; 饶志明

    2012-01-01

    A strain producing red-pigment was newly isolated under poor nutrition conditions. It was identified by 16S rDNA sequence and systematic analysis,as well as general morphological and biochemical characteristics,The results show that 16S rDNA sequence of the strain suggesting that the strain is Serratia marcescens Species. And it was named Serratia marcescens JNB5-1. The red-pigment was identified as prodigiosin by wavelength scanning and LC-MS. preliminary fermentation conditions of strain Serratia marcescens JNB5-1 was studied.The results showed that the yield of prodigiosin was improved to 4.139 g/L after 72 h in the medium:sucrose 2%,beef extract 1.5%, CaCl2 1%,proline 0.75%,MgSO4-7HIO 0.02%,FeSO4-7H2O 0.006%.%利用贫营养条件,从土壤样品中筛选到一株产红色素的菌株.经形态学、生理生化实验进行菌株初步鉴定,并经16S rDNA测序分析确定该菌株为粘质沙雷氏菌属,将其命名为:Serratia marcescens JNB5-1.该菌株所产红色素经全波长扫描及LC-MS确定为灵菌红素.对Serratia marcecens JNB5-1产灵菌红素做初步发酵研究,在蔗糖2g/dL,牛肉膏1.5 g/dL,CaCl21 g/dL,脯氨酸0.75 g/dL,MgSO4·7H2O 0.02 g/dL,FeSO4·7H2O 0.006 g/dL的培养基中发酵72 h后,其发酵产量可达4.139 g/L.

  13. [The use of nitric oxide during transport of newborns with critical respiratory insufficiency: own experience, preliminary report].

    Science.gov (United States)

    Ziebiński, Marek; Walas, Wojciech

    2002-01-01

    This preliminary report presents author's experience with inhaled nitric oxide during transport of newborns with critical respiratory insufficiency. The theoretical basis, indications and contraindications as well as principles of administration during transport are described. The required equipment and some technical aspects are discussed. A short preview of performed transportations is given. Preliminary data show, that use of NO during transport is very helpful in children with critical respiratory insufficiency.

  14. Preliminary investigation into the possible endocrine disrupting ...

    African Journals Online (AJOL)

    JTEkanem

    In this study, we quantified the effect of Bonny- light crude oil contaminated diet ... This preliminary result suggest that Bonny-Light crude oil may have the potential .... different components. Its exploration ... organisms found in crude oil polluted.

  15. 42 CFR 457.925 - Preliminary investigation.

    Science.gov (United States)

    2010-10-01

    ... (CONTINUED) STATE CHILDREN'S HEALTH INSURANCE PROGRAMS (SCHIPs) ALLOTMENTS AND GRANTS TO STATES Program Integrity § 457.925 Preliminary investigation. If the State agency receives a complaint of fraud or abuse...

  16. Preliminary project proposal : Missouri chutes, NE & IA

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This proposal is for the Missouri Chutes National Wildlife Refuge that would be in Nebraska and Iowa on the Missouri River. This preliminary project proposal focuses...

  17. Preliminary Survey of Ectoparasites Infesting Chickens (Gallus ...

    African Journals Online (AJOL)

    Preliminary Survey of Ectoparasites Infesting Chickens (Gallus domesticus) in. Four Areas of ... were identified with the following prevalences: the shaft louse, Menopon gallinae (8.1%), the chicken ..... Canis lupus familiaris in Mueang district ...

  18. Renewable Energy Park - Preliminary Feasibility & Engineering Report

    Energy Technology Data Exchange (ETDEWEB)

    Ariwite, Roderick [Fallon Paiute Shoshone Tribe, NV (United States)

    2015-07-31

    This "Renewable Energy Park - Preliminary Feasibility & Engineering Report" seeks to provide an overall assessment and review of renewable energy development opportunities on the Fallon Indian Reservation and Colony Lands.

  19. Preliminary Determination of Epicenters (PDE) Bulletin

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The NEIC global earthquake bulletin is called the Preliminary Determination of Epicenters or PDE, and is one of many discrete products in the ANSS Comprehensive...

  20. Alaska gold rush trails study: Preliminary draft

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Preliminary study draft, with maps, of seven gold rush trails in Alaska, to determine suitability for inclusion in the National Scenic Trails system and their...

  1. Waterfowl identification workshops

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Summary of waterfowl identification workshops conducted by Upper Souris National Wildlife Refuge and J. Clark Salyer National Wildlife Refuge. Objectives of the...

  2. Optimal Inputs for System Identification.

    Science.gov (United States)

    1995-09-01

    The derivation of the power spectral density of the optimal input for system identification is addressed in this research. Optimality is defined in...identification potential of general System Identification algorithms, a new and efficient System Identification algorithm that employs Iterated Weighted Least

  3. Geochemical identification of projectiles in impact rocks

    Science.gov (United States)

    Tagle, Roald; Hecht, Lutz

    2006-11-01

    The three major geochemical methods for impactor identification are evaluated with respect to their potential and limitations with regards to the precise detection and identification of meteoritic material in impactites. The identification of a projectile component in impactites can be achieved by determining certain isotopic and elemental ratios in contaminated impactites. The isotopic methods are based on Os and Cr isotopic ratios. Osmium isotopes are highly sensitive for the detection of minute amounts of extraterrestrial components of even isotopic method requires the relatively highest projectile contamination (several wt%) in order to detect an extraterrestrial component, but may allow the identification of three different groups of extraterrestrial materials, ordinary chondrites, an enstatite chondrites, and differentiated achondrites. A significant advantage of this method is its independence of the target lithology and post-impact alteration. The use of elemental ratios, including platinum group elements (PGE: Os, Ir, Ru, Pt, Rh, Pd), in combination with Ni and Cr represents a very powerful method for the detection and identification of projectiles in terrestrial and lunar impactites. For most projectile types, this method is almost independent of the target composition, especially if PGE ratios are considered. This holds true even in cases of terrestrial target lithologies with a high component of upper mantle material. The identification of the projectile is achieved by comparison of the "projectile elemental ratio" derived from the slope of the mixing line (target-projectile) with the elemental ratio in the different types of possible projectiles (e.g., chondrites). However, this requires a set of impactite samples of various degree of projectile contamination.

  4. Preliminary organizational culture scale focused on artifacts.

    Science.gov (United States)

    Bonavia, Tomas

    2006-12-01

    In this preliminary study, an Organizational Culture Scale was developed to assess cultural artifacts according to Schein's typology (1985). It includes a set of cultural artifacts to measure the extent to which an organization is more or less traditional. A total of 249 managers from a range of different companies responded to the items. Preliminary analysis yielded a one-dimensional scale with 14 items with high internal consistency and homogeneity.

  5. Preliminary organizational culture scale focused on artifacts

    OpenAIRE

    Bonavia, Tomas

    2006-01-01

    In this preliminary study, an organizational culture scale was developed to assess cultural artifacts according to Schein´s typology (1985). It includes a set of cultural artifacts to measure the extent to which an organization is more or less traditional. A total of 249 managers from a range of different companies responded to the items. Preliminary analysis yielded a one-dimensional scale with 14 items with high internal consistency and homogeneity.

  6. Phytoplankton Identification Manual

    Digital Repository Service at National Institute of Oceanography (India)

    Verlecar, X.N.; Desai, S.R.

    of Environment & Forests, New Delhi 3 FOREWORD Since its inception in 1966 the National Institute of Oceanography is involved in taxonomic classification of marine phytoplankton, zooplankton, benthos and other flora and fauna under the Project ? Measurement... details of taxonomic classification and description of the concerned organisms / species. All the figures are well illustrated and detailed identification key is provided. This should surely guide even a beginner to understand the identification...

  7. 基于脏腑相关理论的亚健康状态中医体质辨识思路初探%A preliminary study of TCM constitution identification of subhealth state based on the theory of internal organs

    Institute of Scientific and Technical Information of China (English)

    邱新萍; 徐彧; 王洪; 魏青; 苏华巍; 邱新红; 仇军; 杨雪卿; 郭华; 孙颂歌

    2016-01-01

    马万千老中医认为亚健康状态是介于生理和病理体质的中间状态,亚健康状态是否进展到疾病状态与脏腑功能强弱、气血、阴阳失衡关系密切。马老认为,通过中医体质辨识和脏腑相关理论结合能够及早发现亚健康状态者的脏腑功能强弱及气血、阴阳偏颇失衡的体质状态,在饮食、运动、情志方面进行早期个性化干预指导,有效纠正失衡之态,从而达到“治未病”的目的。%The old doctor of Traditional Chinese Medicine MA Wan-qian thinks that sub-health state is the intermediate state of physiological and pathological constitution, the subhealth state whether progress to disease states and viscera function is closely related to strength, Qi and blood, imbalance of Yin and Yang. MA believes that the constitution of TCM identification can help recognize the early detection of the organ function strength and physical state of Qi and blood, Yin and Yang of sub-health people organ and give guidance, in the diet, exercise, emotion, effective correcting imbalances of tai, so as to achieve the aim of "preventive treatment of disease".

  8. On-orbit parametric identification methodology

    Science.gov (United States)

    Hadaegh, Fred Y.; Bayard, David S.

    1988-01-01

    On-orbit system identification (ID) of large space systems is essential for various reasons. For example, the complex composite structure of such systems cannot be ground-tested; their structural dynamic characteristics must be known accurately in order to accomplish active control. Furthermore, such capability can be used to characterize/identify various disturbances. The identification process is consisted of four principal elements: (1) modeling, (2) the estimation algorithm, (3) input system, and (4) measurement system. These elements are highly correlated and all togerher determine the success of the identification problem. Accurate modeling of large space systems is the most important element of the identification process. Large flexible structures are non-linear and infinite dimensional systems with highly coupled parameters and low frequency packed modes. In addition, these systems are subject to stochastic and time-varying disturbances, they have structural parameters which can vary due to on-orbit assembly deployment, and operations. These systems are generally; however, represented by constant coefficient, finite order differential equations. The non-linearities, coupling and noise effects are also often neglected. Moreover, identification experiment designs which lead to highly complex optimization problems usually require the simultaneous choice of ID algorithm, sensor, and actuator type and placement. On-orbit bandwidth and power restrictions on excitation, limited data window, and restrictions on sensor/actuator type, placement and number, has led to practical questions of implementations.

  9. Methodology for Preliminary Design of Electrical Microgrids

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, Richard P. [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Stamp, Jason E. [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Eddy, John P. [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Henry, Jordan M [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Munoz-Ramos, Karina [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Abdallah, Tarek [U.S. Army Corps of Engineers, Washington, DC (United States)

    2015-09-30

    Many critical loads rely on simple backup generation to provide electricity in the event of a power outage. An Energy Surety Microgrid TM can protect against outages caused by single generator failures to improve reliability. An ESM will also provide a host of other benefits, including integration of renewable energy, fuel optimization, and maximizing the value of energy storage. The ESM concept includes a categorization for microgrid value proposi- tions, and quantifies how the investment can be justified during either grid-connected or utility outage conditions. In contrast with many approaches, the ESM approach explic- itly sets requirements based on unlikely extreme conditions, including the need to protect against determined cyber adversaries. During the United States (US) Department of Defense (DOD)/Department of Energy (DOE) Smart Power Infrastructure Demonstration for Energy Reliability and Security (SPIDERS) effort, the ESM methodology was successfully used to develop the preliminary designs, which direct supported the contracting, construction, and testing for three military bases. Acknowledgements Sandia National Laboratories and the SPIDERS technical team would like to acknowledge the following for help in the project: * Mike Hightower, who has been the key driving force for Energy Surety Microgrids * Juan Torres and Abbas Akhil, who developed the concept of microgrids for military installations * Merrill Smith, U.S. Department of Energy SPIDERS Program Manager * Ross Roley and Rich Trundy from U.S. Pacific Command * Bill Waugaman and Bill Beary from U.S. Northern Command * Melanie Johnson and Harold Sanborn of the U.S. Army Corps of Engineers Construc- tion Engineering Research Laboratory * Experts from the National Renewable Energy Laboratory, Idaho National Laboratory, Oak Ridge National Laboratory, and Pacific Northwest National Laboratory

  10. Analytical Chemistry Core Capability Assessment - Preliminary Report

    Energy Technology Data Exchange (ETDEWEB)

    Barr, Mary E. [Los Alamos National Laboratory; Farish, Thomas J. [Los Alamos National Laboratory

    2012-05-16

    The concept of 'core capability' can be nebulous one. Even at a fairly specific level, where core capability equals maintaining essential services, it is highly dependent upon the perspective of the requestor. Samples are submitted to analytical services because the requesters do not have the capability to conduct adequate analyses themselves. Some requests are for general chemical information in support of R and D, process control, or process improvement. Many analyses, however, are part of a product certification package and must comply with higher-level customer quality assurance requirements. So which services are essential to that customer - just those for product certification? Does the customer also (indirectly) need services that support process control and improvement? And what is the timeframe? Capability is often expressed in terms of the currently utilized procedures, and most programmatic customers can only plan a few years out, at best. But should core capability consider the long term where new technologies, aging facilities, and personnel replacements must be considered? These questions, and a multitude of others, explain why attempts to gain long-term consensus on the definition of core capability have consistently failed. This preliminary report will not try to define core capability for any specific program or set of programs. Instead, it will try to address the underlying concerns that drive the desire to determine core capability. Essentially, programmatic customers want to be able to call upon analytical chemistry services to provide all the assays they need, and they don't want to pay for analytical chemistry services they don't currently use (or use infrequently). This report will focus on explaining how the current analytical capabilities and methods evolved to serve a variety of needs with a focus on why some analytes have multiple analytical techniques, and what determines the infrastructure for these analyses. This

  11. Preliminary classification criteria for the cryoglobulinaemic vasculitis

    Science.gov (United States)

    De Vita, S; Soldano, F; Isola, M; Monti, G; Gabrielli, A; Tzioufas, A; Ferri, C; Ferraccioli, G F; Quartuccio, L; Corazza, L; De Marchi, G; Casals, M Ramos; Voulgarelis, M; Lenzi, M; Saccardo, F; Fraticelli, P; Mascia, M T; Sansonno, D; Cacoub, P; Tomsic, M; Tavoni, A; Pietrogrande, M; Zignego, A L; Scarpato, S; Mazzaro, C; Pioltelli, P; Steinfeld, S; Lamprecht, P; Bombardieri, S; Galli, M

    2011-01-01

    Background To develop preliminary classification criteria for the cryoglobulinaemic syndrome or cryoglobulinaemic vasculitis (CV). Methods Study part I developed a questionnaire for CV to be included in the formal, second part (study part II). Positivity of serum cryoglobulins was defined by experts as an essential condition for CV classification. In study part II, a core set of classification items (questionnaire, clinical and laboratory items, as agreed) was tested in three groups of patients and controls—that is, group A (new patients with the CV), group B (controls with serum cryoglobulins but lacking CV) and group C (controls without serum cryoglobulins but with features which can be observed in CV). Results In study part I (188 cases, 284 controls), a positive response to at least two of three selected questions showed a sensitivity of 81.9% and a specificity of 83.5% for CV. This questionnaire was employed and validated in study part II, which included 272 patients in group A and 228 controls in group B. The final classification criteria for CV, by pooling data from group A and group B, required the positivity of questionnaire plus clinical, questionnaire plus laboratory, or clinical plus laboratory items, or all the three, providing a sensitivity of 88.5% and a specificity of 93.6% for CV. By comparing data in group A versus group C (425 controls), the same classification criteria showed a sensitivity 88.5% and a specificity 97.0% for CV. Conclusion Classification criteria for CV were developed, and now need validation. PMID:21571735

  12. Multimodal Person Re-identification Using RGB-D Sensors and a Transient Identification Database

    DEFF Research Database (Denmark)

    Møgelmose, Andreas; Moeslund, Thomas B.; Nasrollahi, Kamal

    2013-01-01

    . Subjects are added to a transient database and re-identified based on the distance between recorded biometrics and the currently measured metrics. The system works on live video and requires no collaboration from the subjects. The system achieves a 68% re-identification rate with no wrong re...

  13. Affordable Development and Demonstration of a Small NTR Engine and Stage: A Preliminary NASA, DOE, and Industry Assessment

    Science.gov (United States)

    Borowski, Stanley K.; Sefcik, Robert J.; Fittje, James E.; McCurdy, David R.; Qualls, Arthur L.; Schnitzler, Bruce G.; Werner, James E.; Weitzberg, Abraham; Joyner, Claude R.

    2015-01-01

    The Nuclear Thermal Rocket (NTR) represents the next evolutionary step in cryogenic liquid rocket engines. Deriving its energy from fission of uranium-235 atoms contained within fuel elements that comprise the engine's reactor core, the NTR can generate high thrust at a specific impulse of approx. 900 seconds or more - twice that of today's best chemical rockets. In FY'11, as part of the AISP project, NASA proposed a Nuclear Thermal Propulsion (NTP) effort that envisioned two key activities - "Foundational Technology Development" followed by system-level "Technology Demonstrations". Five near-term NTP activities identified for Foundational Technology Development became the basis for the NCPS project started in FY'12 and funded by NASA's AES program. During Phase 1 (FY'12-14), the NCPS project was focused on (1) Recapturing fuel processing techniques and fabricating partial length "heritage" fuel elements for the two candidate fuel forms identified by NASA and the DOE - NERVA graphite "composite" and the uranium dioxide (UO2) in tungsten "cermet". The Phase 1 effort also included: (2) Engine Conceptual Design; (3) Mission Analysis and Requirements Definition; (4) Identification of Affordable Options for Ground Testing; and (5) Formulation of an Affordable and Sustainable NTP Development Strategy. During FY'14, a preliminary plan for DDT&E was outlined by GRC, the DOE and industry for NASA HQ that involved significant system-level demonstration projects that included GTD tests at the NNSS, followed by a FTD mission. To reduce development costs, the GTD and FTD tests use a small, low thrust (approx. 7.5 or 16.5 klbf) engine. Both engines use graphite composite fuel and a "common" fuel element design that is scalable to higher thrust (approx. 25 klbf) engines by increasing the number of elements in a larger diameter core that can produce greater thermal power output. To keep the FTD mission cost down, a simple "1-burn" lunar flyby mission was considered along with

  14. 78 FR 15709 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Science.gov (United States)

    2013-03-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XC512 Schedules for Atlantic Shark Identification... of public workshops. SUMMARY: Free Atlantic Shark Identification Workshops and Protected Species Safe... fishermen and shark dealers are required to attend a workshop to meet regulatory requirements and...

  15. 75 FR 29991 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Science.gov (United States)

    2010-05-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XW44 Schedules for Atlantic Shark Identification... of public workshops. SUMMARY: Free Atlantic Shark Identification Workshops and Protected Species Safe.... Certain fishermen and shark dealers are required to attend a workshop to meet regulatory requirements...

  16. 78 FR 34349 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Science.gov (United States)

    2013-06-07

    ... National Oceanic and Atmospheric Administration RIN 0648-XC681 Schedules for Atlantic Shark Identification... of public workshops. SUMMARY: Free Atlantic Shark Identification Workshops and Protected Species Safe.... Certain fishermen and shark dealers are required to attend a workshop to meet regulatory requirements...

  17. 77 FR 55464 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Science.gov (United States)

    2012-09-10

    ... National Oceanic and Atmospheric Administration RIN 0648-XC174 Schedules for Atlantic Shark Identification... of public workshops. SUMMARY: Free Atlantic Shark Identification Workshops and Protected Species Safe.... Certain fishermen and shark dealers are required to attend a workshop to meet regulatory requirements...

  18. 77 FR 12574 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Science.gov (United States)

    2012-03-01

    ... National Oceanic and Atmospheric Administration RIN 0648-XB037 Schedules for Atlantic Shark Identification... of public workshops. SUMMARY: Free Atlantic Shark Identification Workshops and Protected Species Safe... fishermen and shark dealers are required to attend a workshop to meet regulatory requirements and...

  19. 77 FR 73451 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Science.gov (United States)

    2012-12-10

    ... National Oceanic and Atmospheric Administration RIN 0648-XC361 Schedules for Atlantic Shark Identification... of public workshops. SUMMARY: Free Atlantic Shark Identification Workshops and Protected Species Safe.... Certain fishermen and shark dealers are required to attend a workshop to meet regulatory requirements...

  20. 78 FR 54456 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Science.gov (United States)

    2013-09-04

    ... National Oceanic and Atmospheric Administration RIN 0648-XC810 Schedules for Atlantic Shark Identification... of public workshops. SUMMARY: Free Atlantic Shark Identification Workshops and Protected Species Safe.... Certain fishermen and shark dealers are required to attend a workshop to meet regulatory requirements...