WorldWideScience

Sample records for preliminary kinetic analysis

  1. Laboratory evaluation of the pivot-shift phenomenon with use of kinetic analysis: a preliminary study.

    Science.gov (United States)

    Sanchis-Alfonso, Vicente; Baydal-Bertomeu, José-María; Castelli, Andrea; Montesinos-Berry, Erik; Marín-Roca, Susana; Garrido-Jaén, José-David

    2011-07-06

    Currently, a suitable and reliable noninvasive method to evaluate rotational stability in vivo in anterior cruciate ligament-deficient knees, particularly during sports movements, does not exist. We speculated that if there is a rotational instability, the patient would avoid reaching a high pivoting moment during pivoting activities as a defense mechanism, and that the ground reaction moment, as registered by dynamometric platforms, would be reduced. On the basis of this hypothesis, we developed a study using kinetic analysis to evaluate rotational stability under dynamic loading. Thirty recreationally active athletes, including fifteen healthy subjects and fifteen with an anterior cruciate ligament-deficient knee, were recruited for this study. Patients performed jumping with pivoting with internal tibial rotation and external tibial rotation on the dynamometric platform with both the healthy and the injured limb. The quantitative results were graphically plotted, and the following parameters were evaluated: loading moment, pivoting moment, torque amplitude, loading slope, pivoting slope, percentage of pivoting with load, loading impulse, pivoting impulse, and maximum body rotation angle. There were no significant differences between the dominant and nondominant knees in the control group during the jumping with pivoting and external tibial rotation test with regard to the pivoting moment (p = 0.805), pivoting slope (p = 0.716), pivoting impulse 2 (p = 0.858), and pivoting impulse 3 (p = 0.873). In patients with a chronic tear of the anterior cruciate ligament, there was a significant decrease of the pivoting moment (p = 0.02), pivoting slope (p = 0.005), pivoting impulse 2 (p = 0.006), and pivoting impulse 3 (p = 0.035) during the jumping with pivoting and external tibial rotation test in the anterior cruciate ligament-deficient knee compared with the healthy, contralateral knee. Kinetic analysis with use of a dynamic platform can objectively detect alterations

  2. The preliminary analysis on the steady-state and kinetic features of the molten salt pebble-bed reactor

    Energy Technology Data Exchange (ETDEWEB)

    Xia, B. [Inst. of Nuclear and New Energy Technology, Tsinghua Univ., Beijing 100084 (China); Lu, Y. [Green Hi-Tek, 104 Harland Court, Oak Ridge, TN 37830 (United States)

    2012-07-01

    A novel design concept of molten salt pebble-bed reactor with an ultra-simplified integral primary circuit called 'Nuclear Hot Spring' has been proposed, featured by horizontal coolant flow in a deep pool pebble-bed reactor, providing 'natural safety' features with natural circulation under full power operation and less expensive primary circuit arrangement. In this work, the steady-state physical properties of the equilibrium state of the molten salt pebble-bed reactor are calculated by using the VSOP code, and the steady-state thermo-hydraulic analysis is carried out based on the approximation of absolutely horizontal flow of the coolant through the core. A new concept of 2-dimensional, both axial and radial, multi-pass on-line fuelling scheme is presented. The result reveals that the radial multi-pass scheme provides more flattened power distribution and safer temperature distribution than the one-pass scheme. A parametric analysis is made corresponding to different pebble diameters, the key parameter of the core resistance and the temperature at the pebble center. It is verified that within a wide range of pebble diameters, the maximum pebble center temperatures are far below the safety limit of the fuel, and the core resistance is considerably less than the buoyant force, indicating that the natural circulation under full power operation is achievable and the ultra-simplified integral primary circuit without any pump is possible. For the kinetic properties, it is verified that the negative temperature coefficient is achieved in sufficient under-moderated condition through the preliminary analysis on the temperature coefficients of fuel, coolant and moderator. The requirement of reactivity compensation at the shutdown stages of the operation period is calculated for the further studies on the reactivity control. The molten salt pebble-bed reactor with horizontal coolant flow can provide enhanced safety and economical features. (authors)

  3. Combination Of Static And Dynami,C Stereophotogrammetry For The Kinetic Analysis Of Human Locomotion: Preliminary Results

    Science.gov (United States)

    Schaer, Alex R.; Sheffer, Daniel B.; Jones, D.; Meier, G.; Baumann, Juerg U.

    1989-04-01

    For a deeper understanding of the complexity of human walking movement not only a kinematic analysis , but also a comprehensive three-dimensional biomechanical model of the human body is required to detail the kinetic activities. This research combined static stereophotogrammetric determination of body segment mass parameters with three-dimensional gait analysis by cinephotography, direct linear transformation and two force plates. A method of combining the two independent analyses by defining the anatomical axes of each segment is shown. Practical problems arising in dynamic and stereometric analysis are demonstrated. Power spectra of a normal and a matched subject with spastic diplegia were calculated for a proper design of the kinematic analysis.

  4. Preliminary study on mechanics-based rainfall kinetic energy

    Directory of Open Access Journals (Sweden)

    Yuan Jiuqin Ms.

    2014-09-01

    Full Text Available A raindrop impact power observation system was employed to observe the real-time raindrop impact power during a rainfall event and to analyze the corresponding rainfall characteristics. The experiments were conducted at different simulated rainfall intensities. As rainfall intensity increased, the observed impact power increased linearly indicating the power observation system would be satisfactory for characterizing rainfall erosivity. Momentum is the product of mass and velocity (Momentum=MV, which is related to the observed impact power value. Since there is no significant difference between momentum and impact power, observed impact power can represent momentum for different rainfall intensities. The relationship between momentum and the observed impact power provides a convenient way to calculate rainfall kinetic energy. The value of rainfall kinetic energy based on the observed impact power was higher than the classic rainfall kinetic energy. The rainfall impact power based kinetic energy and the classic rainfall kinetic energy showed linear correlation, which indicates that the raindrop impact power observation system can characterize rainfall kinetic energy. The article establishes a preliminary way to calculate rainfall kinetic energy by using the real-time observed momentum, providing a foundation for replacing the traditional methods for estimating kinetic energy of rainstorms.

  5. Adsorption analysis equilibria and kinetics

    CERN Document Server

    Do, Duong D

    1998-01-01

    This book covers topics of equilibria and kinetics of adsorption in porous media. Fundamental equilibria and kinetics are dealt with for homogeneous as well as heterogeneous particles. Five chapters of the book deal with equilibria and eight chapters deal with kinetics. Single component as well as multicomponent systems are discussed. In kinetics analysis, we deal with the various mass transport processes and their interactions inside a porous particle. Conventional approaches as well as the new approach using Maxwell-Stefan equations are presented. Various methods to measure diffusivity, such

  6. UVISS preliminary visibility analysis

    DEFF Research Database (Denmark)

    Betto, Maurizio

    1998-01-01

    The goal of this work is to obtain a preliminary assessment of the sky visibility for anastronomical telescope located on the express pallet of the International SpaceStation (ISS)} taking into account the major constraints imposed on the instrument by the ISSattitude and structure. Part of the w......The goal of this work is to obtain a preliminary assessment of the sky visibility for anastronomical telescope located on the express pallet of the International SpaceStation (ISS)} taking into account the major constraints imposed on the instrument by the ISSattitude and structure. Part...

  7. UVISS preliminary visibility analysis

    DEFF Research Database (Denmark)

    Betto, Maurizio

    1998-01-01

    The goal of this work is to obtain a preliminary assessment of the sky visibility for anastronomical telescope located on the express pallet of the International SpaceStation (ISS)} taking into account the major constraints imposed on the instrument by the ISSattitude and structure. Part...

  8. Concept Overview & Preliminary Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Ruth, Mark

    2017-07-12

    'H2@Scale' is an opportunity for wide-scale use of hydrogen as an intermediate that carries energy from various production options to multiple uses. It is based on identifying and developing opportunities for low-cost hydrogen production and investigating opportunities for using that hydrogen across the electricity, industrial, and transportation sectors. One of the key production opportunities is use of low-cost electricity that may be generated under high penetrations of variable renewable generators such as wind and solar photovoltaics. The technical potential demand for hydrogen across the sectors is 60 million metric tons per year. The U.S. has sufficient domestic renewable resources so that each could meet that demand and could readily meet the demand using a portfolio of generation options. This presentation provides an overview of the concept and the technical potential demand and resources. It also motivates analysis and research on H2@Scale.

  9. Preliminary hazards analysis -- vitrification process

    Energy Technology Data Exchange (ETDEWEB)

    Coordes, D.; Ruggieri, M.; Russell, J.; TenBrook, W.; Yimbo, P. [Science Applications International Corp., Pleasanton, CA (United States)

    1994-06-01

    This paper presents a Preliminary Hazards Analysis (PHA) for mixed waste vitrification by joule heating. The purpose of performing a PHA is to establish an initial hazard categorization for a DOE nuclear facility and to identify those processes and structures which may have an impact on or be important to safety. The PHA is typically performed during and provides input to project conceptual design. The PHA is then followed by a Preliminary Safety Analysis Report (PSAR) performed during Title 1 and 2 design. The PSAR then leads to performance of the Final Safety Analysis Report performed during the facility`s construction and testing. It should be completed before routine operation of the facility commences. This PHA addresses the first four chapters of the safety analysis process, in accordance with the requirements of DOE Safety Guidelines in SG 830.110. The hazards associated with vitrification processes are evaluated using standard safety analysis methods which include: identification of credible potential hazardous energy sources; identification of preventative features of the facility or system; identification of mitigative features; and analyses of credible hazards. Maximal facility inventories of radioactive and hazardous materials are postulated to evaluate worst case accident consequences. These inventories were based on DOE-STD-1027-92 guidance and the surrogate waste streams defined by Mayberry, et al. Radiological assessments indicate that a facility, depending on the radioactive material inventory, may be an exempt, Category 3, or Category 2 facility. The calculated impacts would result in no significant impact to offsite personnel or the environment. Hazardous materials assessment indicates that a Mixed Waste Vitrification facility will be a Low Hazard facility having minimal impacts to offsite personnel and the environment.

  10. Analysis of kinetic reaction mechanisms

    CERN Document Server

    Turányi, Tamás

    2014-01-01

    Chemical processes in many fields of science and technology, including combustion, atmospheric chemistry, environmental modelling, process engineering, and systems biology, can be described by detailed reaction mechanisms consisting of numerous reaction steps. This book describes methods for the analysis of reaction mechanisms that are applicable in all these fields. Topics addressed include: how sensitivity and uncertainty analyses allow the calculation of the overall uncertainty of simulation results and the identification of the most important input parameters, the ways in which mechanisms can be reduced without losing important kinetic and dynamic detail, and the application of reduced models for more accurate engineering optimizations. This monograph is invaluable for researchers and engineers dealing with detailed reaction mechanisms, but is also useful for graduate students of related courses in chemistry, mechanical engineering, energy and environmental science and biology.

  11. A Preliminary Study of the Potential to Kinetic Energy Conversion Process in the Stratosphere

    OpenAIRE

    White, Robert M.; Nolan, George F.

    2011-01-01

    The potential to kinetic energy conversion process in the lower stratosphere associated with the vertical exchange of warm and cold air is evaluated using adiabatically derived vertical velocities for the North American region for a five-day period. Preliminary results suggest the possibility that on the average the kinetic energy of stratospheric motions may not result from a conversion of potential energy within the stratosphere by this process. The further implication is that stratospheric...

  12. Robustness Analysis of Kinetic Structures

    DEFF Research Database (Denmark)

    Kirkegaard, Poul Henning; Sørensen, John Dalsgaard

    2009-01-01

    The present paper considers robustness of kinetic structures. Robustness of structures has obtained a renewed interest due to a much more frequent use of advanced types of structures with limited redundancy and serious consequences in case of failure. Especially for these types of structural syst...... systems, it is of interest to investigate how robust the structures are, or what happens if a structural element is added to or removed from the original structure. The present paper discusses this issue for kinetic structures in architecture....

  13. Robustness Analysis of Kinetic Structures

    DEFF Research Database (Denmark)

    Kirkegaard, Poul Henning; Sørensen, John Dalsgaard

    2009-01-01

    Kinetic structures in architecture follows a new trend which is emerging in responsive architecture coined by Nicholas Negroponte when he proposed that architecture may benefit from the integration of computing power into built spaces and structures, and that better performing, more rational...

  14. Robustness Analysis of Kinetic Structures

    DEFF Research Database (Denmark)

    Kirkegaard, Poul Henning; Sørensen, John Dalsgaard

    2009-01-01

    Kinetic structures in architecture follows a new trend which is emerging in responsive architecture coined by Nicholas Negroponte when he proposed that architecture may benefit from the integration of computing power into built spaces and structures, and that better performing, more rational...

  15. Hydrothermal Liquefaction Treatment Preliminary Hazard Analysis Report

    Energy Technology Data Exchange (ETDEWEB)

    Lowry, Peter P.; Wagner, Katie A.

    2015-08-31

    A preliminary hazard assessment was completed during February 2015 to evaluate the conceptual design of the modular hydrothermal liquefaction treatment system. The hazard assessment was performed in 2 stages. An initial assessment utilizing Hazard Identification and Preliminary Hazards Analysis (PHA) techniques identified areas with significant or unique hazards (process safety-related hazards) that fall outside of the normal operating envelope of PNNL and warranted additional analysis. The subsequent assessment was based on a qualitative What-If analysis. This analysis was augmented, as necessary, by additional quantitative analysis for scenarios involving a release of hazardous material or energy with the potential for affecting the public.

  16. MBS Analysis Of Kinetic Structures Using ADAMS

    DEFF Research Database (Denmark)

    Kirkegaard, Poul Henning; Nielsen, Søren R.K.

    2009-01-01

    The present paper considers multibody system (MBS) analysis of kinetic structures using the software package ADAMS. Deployable, foldable, expandable and reconfigurable kinetic structures can provide a change in the geometric morphology of the envelope by contributing to making it adaptable to e.......g. changing external climate factors, in order to improve the indoor climate performance of the building. The derivation of equations of motion for such spatial mechanical systems is a challenging issue in scientific community. However, with new symbolic tools one can automatically derive equations in so......-called multibody system (MBS) formalism. The present paper considers MBS modeling of kinetic architectural structures using the software packages ADAMS. As a result, it is found that symbolic MBS simulation tools facilitate a useful evaluation environment for MBS users during a design phase of responsive kinetic...

  17. Breast DCE-MRI Kinetic Heterogeneity Tumor Markers: Preliminary Associations With Neoadjuvant Chemotherapy Response1

    Science.gov (United States)

    Ashraf, Ahmed; Gaonkar, Bilwaj; Mies, Carolyn; DeMichele, Angela; Rosen, Mark; Davatzikos, Christos; Kontos, Despina

    2015-01-01

    The ability to predict response to neoadjuvant chemotherapy for women diagnosed with breast cancer, either before or early on in treatment, is critical to judicious patient selection and tailoring the treatment regimen. In this paper, we investigate the role of contrast agent kinetic heterogeneity features derived from breast dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for predicting treatment response. We propose a set of kinetic statistic descriptors and present preliminary results showing the discriminatory capacity of the proposed descriptors for predicting complete and non-complete responders as assessed from pre-treatment imaging exams. The study population consisted of 15 participants: 8 complete responders and 7 non-complete responders. Using the proposed kinetic features, we trained a leave-one-out logistic regression classifier that performs with an area under the receiver operating characteristic (ROC) curve (AUC) of 0.84 under the ROC. We compare the predictive value of our features against commonly used MRI features including kinetics of the characteristic kinetic curve (CKC), maximum peak enhancement (MPE), hotspot signal enhancement ratio (SER), and longest tumor diameter that give lower AUCs of 0.71, 0.66, 0.64, and 0.54, respectively. Our proposed kinetic statistics thus outperform the conventional kinetic descriptors as well as the classifier using a combination of all the conventional descriptors (i.e., CKC, MPE, SER, and longest diameter), which gives an AUC of 0.74. These findings suggest that heterogeneity-based DCE-MRI kinetic statistics could serve as potential imaging biomarkers for tumor characterization and could be used to improve candidate patient selection even before the start of the neoadjuvant treatment. PMID:26055172

  18. Breast DCE-MRI Kinetic Heterogeneity Tumor Markers: Preliminary Associations With Neoadjuvant Chemotherapy Response

    Directory of Open Access Journals (Sweden)

    Ahmed Ashraf

    2015-06-01

    Full Text Available The ability to predict response to neoadjuvant chemotherapy for women diagnosed with breast cancer, either before or early on in treatment, is critical to judicious patient selection and tailoring the treatment regimen. In this paper, we investigate the role of contrast agent kinetic heterogeneity features derived from breast dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI for predicting treatment response. We propose a set of kinetic statistic descriptors and present preliminary results showing the discriminatory capacity of the proposed descriptors for predicting complete and non-complete responders as assessed from pre-treatment imaging exams. The study population consisted of 15 participants: 8 complete responders and 7 non-complete responders. Using the proposed kinetic features, we trained a leave-one-out logistic regression classifier that performs with an area under the receiver operating characteristic (ROC curve (AUC of 0.84 under the ROC. We compare the predictive value of our features against commonly used MRI features including kinetics of the characteristic kinetic curve (CKC, maximum peak enhancement (MPE, hotspot signal enhancement ratio (SER, and longest tumor diameter that give lower AUCs of 0.71, 0.66, 0.64, and 0.54, respectively. Our proposed kinetic statistics thus outperform the conventional kinetic descriptors as well as the classifier using a combination of all the conventional descriptors (i.e., CKC, MPE, SER, and longest diameter, which gives an AUC of 0.74. These findings suggest that heterogeneity-based DCE-MRI kinetic statistics could serve as potential imaging biomarkers for tumor characterization and could be used to improve candidate patient selection even before the start of the neoadjuvant treatment.

  19. Breast DCE-MRI Kinetic Heterogeneity Tumor Markers: Preliminary Associations With Neoadjuvant Chemotherapy Response.

    Science.gov (United States)

    Ashraf, Ahmed; Gaonkar, Bilwaj; Mies, Carolyn; DeMichele, Angela; Rosen, Mark; Davatzikos, Christos; Kontos, Despina

    2015-06-01

    The ability to predict response to neoadjuvant chemotherapy for women diagnosed with breast cancer, either before or early on in treatment, is critical to judicious patient selection and tailoring the treatment regimen. In this paper, we investigate the role of contrast agent kinetic heterogeneity features derived from breast dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for predicting treatment response. We propose a set of kinetic statistic descriptors and present preliminary results showing the discriminatory capacity of the proposed descriptors for predicting complete and non-complete responders as assessed from pre-treatment imaging exams. The study population consisted of 15 participants: 8 complete responders and 7 non-complete responders. Using the proposed kinetic features, we trained a leave-one-out logistic regression classifier that performs with an area under the receiver operating characteristic (ROC) curve (AUC) of 0.84 under the ROC. We compare the predictive value of our features against commonly used MRI features including kinetics of the characteristic kinetic curve (CKC), maximum peak enhancement (MPE), hotspot signal enhancement ratio (SER), and longest tumor diameter that give lower AUCs of 0.71, 0.66, 0.64, and 0.54, respectively. Our proposed kinetic statistics thus outperform the conventional kinetic descriptors as well as the classifier using a combination of all the conventional descriptors (i.e., CKC, MPE, SER, and longest diameter), which gives an AUC of 0.74. These findings suggest that heterogeneity-based DCE-MRI kinetic statistics could serve as potential imaging biomarkers for tumor characterization and could be used to improve candidate patient selection even before the start of the neoadjuvant treatment.

  20. Preliminary safety analysis methodology for the SMART

    Energy Technology Data Exchange (ETDEWEB)

    Bae, Kyoo Hwan; Chung, Y. J.; Kim, H. C.; Sim, S. K.; Lee, W. J.; Chung, B. D.; Song, J. H. [Korea Atomic Energy Research Institute, Taejeon (Korea)

    2000-03-01

    This technical report was prepared for a preliminary safety analysis methodology of the 330MWt SMART (System-integrated Modular Advanced ReacTor) which has been developed by Korea Atomic Energy Research Institute (KAERI) and funded by the Ministry of Science and Technology (MOST) since July 1996. This preliminary safety analysis methodology has been used to identify an envelope for the safety of the SMART conceptual design. As the SMART design evolves, further validated final safety analysis methodology will be developed. Current licensing safety analysis methodology of the Westinghouse and KSNPP PWRs operating and under development in Korea as well as the Russian licensing safety analysis methodology for the integral reactors have been reviewed and compared to develop the preliminary SMART safety analysis methodology. SMART design characteristics and safety systems have been reviewed against licensing practices of the PWRs operating or KNGR (Korean Next Generation Reactor) under construction in Korea. Detailed safety analysis methodology has been developed for the potential SMART limiting events of main steam line break, main feedwater pipe break, loss of reactor coolant flow, CEA withdrawal, primary to secondary pipe break and the small break loss of coolant accident. SMART preliminary safety analysis methodology will be further developed and validated in parallel with the safety analysis codes as the SMART design further evolves. Validated safety analysis methodology will be submitted to MOST as a Topical Report for a review of the SMART licensing safety analysis methodology. Thus, it is recommended for the nuclear regulatory authority to establish regulatory guides and criteria for the integral reactor. 22 refs., 18 figs., 16 tabs. (Author)

  1. Preliminary Analysis of Google+'s Privacy

    OpenAIRE

    2011-01-01

    In this paper we provide a preliminary analysis of Google+ privacy. We identified that Google+ shares photo metadata with users who can access the photograph and discuss its potential impact on privacy. We also identified that Google+ encourages the provision of other names including maiden name, which may help criminals performing identity theft. We show that Facebook lists are a superset of Google+ circles, both functionally and logically, even though Google+ provides a better user interfac...

  2. Kinetics Analysis of Coconut Shell Pyrolysis

    Institute of Scientific and Technical Information of China (English)

    LIU; Xue-mei; JIANG; Jian-chun; SUN; Kang; XU; Fan; XU; Yu

    2012-01-01

    [Objective] The paper aimed to study kinetics analysis of coconut shell pyrolysis. [Method] Thermo gravimetric analysis was used to study the pyrolysis characteristic of coconut shell at different pyrolysis rates (5, 10, 20 K/min). [Result] The pyrolysis process included 3 stages, water loss, pyrolysis, and thermal condensation. The pyrolysis process can be described through first-order reaction model. With the increasing pyrolysis rate, activation energy in the first stage rose, but activation energy in the second stage reduced. [Conclusion] The study provided theoretical basis for the promotion and application of biomass energy.

  3. Mathematics analysis of polymerase chain reaction kinetic curves.

    Science.gov (United States)

    Sochivko, D G; Fedorov, A A; Varlamov, D A; Kurochkin, V E; Petrov, R V

    2016-01-01

    The paper reviews different approaches to the mathematical analysis of polymerase chain reaction (PCR) kinetic curves. The basic principles of PCR mathematical analysis are presented. Approximation of PCR kinetic curves and PCR efficiency curves by various functions is described. Several PCR models based on chemical kinetics equations are suggested. Decision criteria for an optimal function to describe PCR efficiency are proposed.

  4. A Century of Enzyme Kinetic Analysis, 1913 to 2013

    OpenAIRE

    Johnson, Kenneth A.

    2013-01-01

    This review traces the history and logical progression of methods for quantitative analysis of enzyme kinetics from the 1913 Michaelis and Menten paper to the application of modern computational methods today. Following a brief review of methods for fitting steady state kinetic data, modern methods are highlighted for fitting full progress curve kinetics based upon numerical integration of rate equations, including a re-analysis of the original Michaelis-Menten full time course kinetic data. ...

  5. Reaction kinetic analysis of reactor surveillance data

    Science.gov (United States)

    Yoshiie, T.; Kinomura, A.; Nagai, Y.

    2017-02-01

    In the reactor pressure vessel surveillance data of a European-type pressurized water reactor (low-Cu steel), it was found that the concentration of matrix defects was very high, and a large number of precipitates existed. In this study, defect structure evolution obtained from surveillance data was simulated by reaction kinetic analysis using 15 rate equations. The saturation of precipitation and the growth of loops were simulated, but it was not possible to explain the increase in DBTT on the basis of the defect structures. The sub-grain boundary segregation of solutes was discussed for the origin of the DBTT increase.

  6. A Century of Enzyme Kinetic Analysis, 1913 to 2013

    Science.gov (United States)

    Johnson, Kenneth A.

    2013-01-01

    This review traces the history and logical progression of methods for quantitative analysis of enzyme kinetics from the 1913 Michaelis and Menten paper to the application of modern computational methods today. Following a brief review of methods for fitting steady state kinetic data, modern methods are highlighted for fitting full progress curve kinetics based upon numerical integration of rate equations, including a re-analysis of the original Michaelis-Menten full time course kinetic data. Finally, several illustrations of modern transient state kinetic methods of analysis are shown which enable the elucidation of reactions occurring at the active sites of enzymes in order to relate structure and function. PMID:23850893

  7. A century of enzyme kinetic analysis, 1913 to 2013.

    Science.gov (United States)

    Johnson, Kenneth A

    2013-09-02

    This review traces the history and logical progression of methods for quantitative analysis of enzyme kinetics from the 1913 Michaelis and Menten paper to the application of modern computational methods today. Following a brief review of methods for fitting steady state kinetic data, modern methods are highlighted for fitting full progress curve kinetics based upon numerical integration of rate equations, including a re-analysis of the original Michaelis-Menten full time course kinetic data. Finally, several illustrations of modern transient state kinetic methods of analysis are shown which enable the elucidation of reactions occurring at the active sites of enzymes in order to relate structure and function.

  8. Preliminary Hazards Analysis Plasma Hearth Process

    Energy Technology Data Exchange (ETDEWEB)

    Aycock, M.; Coordes, D.; Russell, J.; TenBrook, W.; Yimbo, P. [Science Applications International Corp., Pleasanton, CA (United States)

    1993-11-01

    This Preliminary Hazards Analysis (PHA) for the Plasma Hearth Process (PHP) follows the requirements of United States Department of Energy (DOE) Order 5480.23 (DOE, 1992a), DOE Order 5480.21 (DOE, 1991d), DOE Order 5480.22 (DOE, 1992c), DOE Order 5481.1B (DOE, 1986), and the guidance provided in DOE Standards DOE-STD-1027-92 (DOE, 1992b). Consideration is given to ft proposed regulations published as 10 CFR 830 (DOE, 1993) and DOE Safety Guide SG 830.110 (DOE, 1992b). The purpose of performing a PRA is to establish an initial hazard categorization for a DOE nuclear facility and to identify those processes and structures which may have an impact on or be important to safety. The PHA is typically performed during and provides input to project conceptual design. The PRA then is followed by a Preliminary Safety Analysis Report (PSAR) performed during Title I and II design. This PSAR then leads to performance of the Final Safety Analysis Report performed during construction, testing, and acceptance and completed before routine operation. Radiological assessments indicate that a PHP facility, depending on the radioactive material inventory, may be an exempt, Category 3, or Category 2 facility. The calculated impacts would result in no significant impact to offsite personnel or the environment. Hazardous material assessments indicate that a PHP facility will be a Low Hazard facility having no significant impacts either onsite or offsite to personnel and the environment.

  9. Kinetic Analysis of tRNA Methylfransferases

    Science.gov (United States)

    Hou, Ya-Ming; Masuda, Isao

    2016-01-01

    Transfer RNA (tRNA) molecules contain many chemical modifications that are introduced after transcription. A major form of these modifications is methyl transfer to bases and backbone groups, using S-adenosyl methionine (AdoMet) as the methyl donor. Each methylation confers a specific advantage to tRNA in structure or in function. A remarkable methylation is to the G37 base on the 3' side of the anticodon to generate m1G37-tRNA, which suppresses frameshift errors during protein synthesis and is therefore essential for cell growth in all three domains of life. This methylation is catalyzed by TrmD in bacteria and by Trm5 in eukaryotes and archaea. Although TrmD and Trm5 catalyze the same methylation reaction, kinetic analysis reveal that these two enzymes are unrelated to each other and are distinct in their reaction mechanism. This chapter summarizes the kinetic assays that are used to reveal the distinction between TrmD and Trm5. Three types of assays are described, the steady-state, the pre-steady-state, and the single turnover assays, which collectively provide the basis for mechanistic investigation of AdoMet-dependent methyl transfer reactions. PMID:26253967

  10. Repository Subsurface Preliminary Fire Hazard Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Richard C. Logan

    2001-07-30

    This fire hazard analysis identifies preliminary design and operations features, fire, and explosion hazards, and provides a reasonable basis to establish the design requirements of fire protection systems during development and emplacement phases of the subsurface repository. This document follows the Technical Work Plan (TWP) (CRWMS M&O 2001c) which was prepared in accordance with AP-2.21Q, ''Quality Determinations and Planning for Scientific, Engineering, and Regulatory Compliance Activities''; Attachment 4 of AP-ESH-008, ''Hazards Analysis System''; and AP-3.11Q, ''Technical Reports''. The objective of this report is to establish the requirements that provide for facility nuclear safety and a proper level of personnel safety and property protection from the effects of fire and the adverse effects of fire-extinguishing agents.

  11. Kinetics experiments and bench-scale system: Background, design, and preliminary experiments

    Energy Technology Data Exchange (ETDEWEB)

    Rofer, C.K.

    1987-10-01

    The project, Supercritical Water Oxidation of Hazardous Chemical Waste, is a Hazardous Waste Remedial Actions Program (HAZWRAP) Research and Development task being carried out by the Los Alamos National Laboratory. Its objective is to obtain information for use in understanding the basic technology and for scaling up and applying oxidation in supercritical water as a viable process for treating a variety of DOE-DP waste streams. This report gives the background and rationale for kinetics experiments on oxidation in supercritical water being carried out as a part of this HAZWRAP Research and Development task. It discusses supercritical fluid properties and their relevance to applying this process to the destruction of hazardous wastes. An overview is given of the small emerging industry based on applications of supercritical water oxidation. Factors that could lead to additional applications are listed. Modeling studies are described as a basis for the experimental design. The report describes plug flow reactor and batch reactor systems, and presents preliminary results. 28 refs., 4 figs., 5 tabs.

  12. Kinetic Analysis of Metal Ions: An Undergraduate Laboratory Experiment.

    Science.gov (United States)

    Williams, Kathryn R.

    1985-01-01

    Reports on the adaptation of a kinetic method of analysis of metal ions for use in an undergraduate teaching laboratory. Background information, procedures used, and analysis of typical results obtained are provided. (JN)

  13. BASE Flexible Array Preliminary Lithospheric Structure Analysis

    Science.gov (United States)

    Yeck, W. L.; Sheehan, A. F.; Anderson, M. L.; Siddoway, C. S.; Erslev, E.; Harder, S. H.; Miller, K. C.

    2009-12-01

    The Bighorns Arch Seismic Experiment (BASE) is a Flexible Array experiment integrated with EarthScope. The goal of BASE is to develop a better understanding of how basement-involved foreland arches form and what their link is to plate tectonic processes. To achieve this goal, the crustal structure under the Bighorn Mountain range, Bighorn Basin, and Powder River Basin of northern Wyoming and southern Montana are investigated through the deployment of 35 broadband seismometers, 200 short period seismometers, 1600 “Texan” instruments using active sources and 800 “Texan” instruments monitoring passive sources, together with field structural analysis of brittle structures. The novel combination of these approaches and anticipated simultaneous data inversion will give a detailed structural crustal image of the Bighorn region at all levels of the crust. Four models have been proposed for the formation of the Bighorn foreland arch: subhorizontal detachment within the crust, lithospheric buckling, pure shear lithospheric thickening, and fault blocks defined by lithosphere-penetrating thrust faults. During the summer of 2009, we deployed 35 broadband instruments, which have already recorded several magnitude 7+ teleseismic events. Through P wave receiver function analysis of these 35 stations folded in with many EarthScope Transportable Array stations in the region, we present a preliminary map of the Mohorovicic discontinuity. This crustal map is our first test of how the unique Moho geometries predicted by the four hypothesized models of basement involved arches fit seismic observations for the Bighorn Mountains. In addition, shear-wave splitting analysis for our first few recorded teleseisms helps us determine if strong lithospheric deformation is preserved under the range. These analyses help lead us to our final goal, a complete 4D (3D spatial plus temporal) lithospheric-scale model of arch formation which will advance our understanding of the mechanisms

  14. Kinetic analysis of the human knee joint

    Directory of Open Access Journals (Sweden)

    E Wiczkowski

    2008-03-01

    Full Text Available The pathology of the calcaneal (Achilles tendon constitutes a serious therapeutical and social problem. Indeed, this tendon is the strongest plantar flexor of the foot that plays an important role in the humangait. Although well known for a long time, no explicit description of the spontaneous subcutaneous rupture of the Achilles tendon can be found in medical or biomechanical literature. So far, neither pathomechanism nor the underlying causes of the tendon’s disruption have been fully elucidated. Many authors concentrate mostly on medical and biological aspects of the condition. The commonly held view is that it is the vascular supply to the tendon that plays a crucial role in pathogenesis of the tendon’s injuries. In fact, the vasculature a change with time and after the age of 30 is significantly reduced leading to the development of regressive alterations within as well as the decrement of the mechanical strength of the tendon. Obviously, interdisciplinary approach encompassing not only medical and biological but also the broadly taken mechanical viewpoint is needed to more comprehensively describe and explain this phenomenon. In the present paper, kinetic analysis of the knee was employed to define the trajectory of the point of initial insertion of the medial head of gastrocnemius, which was then used to determine the point’s route within the motor area extending from the flexion to the full extension of the knee. The obtained data on the trajectory are further utilized to present and define the pathomechanism of the spontaneous rupture of the calcaneal tendon.

  15. Dual-fuel, dual-throat engine preliminary analysis

    Science.gov (United States)

    Obrien, C. J.

    1979-01-01

    A propulsion system analysis of the dual fuel, dual throat engine for launch vehicle applications was conducted. Basic dual throat engine characterization data were obtained to allow vehicle optimization studies to be conducted. A preliminary baseline engine system was defined.

  16. Kinetic Analysis of Char Thermal Deactivation

    DEFF Research Database (Denmark)

    Zolin, Alfredo; Jensen, Anker; Dam-Johansen, Kim

    2001-01-01

    data for all chars obtained from the TGA, kinetic parameters in an annealing model reported in the literature were determined. A shifted Gamma distribution taking into account a positive activation energy at the beginning of deactivation, as well as a lower bound of char reactivity corresponding...

  17. [Tuscan Chronic Care Model: a preliminary analysis].

    Science.gov (United States)

    Barbato, Angelo; Meggiolaro, Angela; Rossi, Luigi; Fioravanti, C; Palermita, F; La Torre, Giuseppe

    2015-01-01

    the aim of this study is to present a preliminary analysis of efficacy and effectiveness of a model of chronically ill care (Chronic Care Model, CCM). the analysis took into account 106 territorial modules, 1016 General Practitioners and 1,228,595 patients. The diagnostic and therapeutic pathways activated (PDTA), involved four chronic conditions, selected according to the prevalence and incidence, in Tuscany Region: Diabetes Mellitus (DM), Heart Failure (SC), Chronic Obstructive Pulmonary Disease (COPD) and stroke. Six epidemiological indicators of process and output were selected, in order to measure the model of care performed, before and after its application: adherence to specific follow-up for each pathology (use of clinical and laboratory indicators), annual average of expenditure per/capita/euro for diagnostic tests, in laboratory and instrumental, average expenditure per/capita/year for specialist visits; hospitalization rate for diseases related to the main pathology, hospitalization rate for long-term complications and rate of access to the emergency department (ED). Data were collected through the database; the differences before and after the intervention and between exposed and unexposed, were analyzed by method "Before-After (Controlled and Uncontrolled) Studies". The impact of the intervention was calculated as DD (difference of the differences). DM management showed an increased adhesion to follow-up (DD: +8.1%), and the use of laboratory diagnostics (DD: +4,9 €/year/pc), less hospitalization for long-term complications and for endocrine related diseases (DD respectively: 5.8/1000 and DD: +1.2/1000), finally a smaller increase of access to PS (DD: -1.6/1000), despite a slight increase of specialistic visits (DD: +0,38 €/year/pc). The management of SC initially showed a rising adherence to follow-up (DD: +2.3%), a decrease of specialist visits (DD:E 1.03 €/year/pc), hospitalization and access to PS for exacerbations (DD: -4.4/1000 and DD: -6

  18. Kinetic analysis of dynamic PET data

    Energy Technology Data Exchange (ETDEWEB)

    Knittel, B.

    1983-12-01

    Our goal is to quantify regional physiological processes such as blood flow and metabolism by means of tracer kinetic modeling and positron emission tomography (PET). Compartmental models are one way of characterizing the behavior of tracers in physiological systems. This paper describes a general method of estimating compartmental model rate constants from measurements of the concentration of tracers in blood and tissue, taken at multiple time intervals. A computer program which applies the method is described, and examples are shown for simulated and actual data acquired from the Donner 280-Crystal Positron Tomograph.

  19. Spectrum Analysis of Some Kinetic Equations

    Science.gov (United States)

    Yang, Tong; Yu, Hongjun

    2016-11-01

    We analyze the spectrum structure of some kinetic equations qualitatively by using semigroup theory and linear operator perturbation theory. The models include the classical Boltzmann equation for hard potentials with or without angular cutoff and the Landau equation with {γ≥q-2}. As an application, we show that the solutions to these two fundamental equations are asymptotically equivalent (mod time decay rate {t^{-5/4}}) as {tto∞} to that of the compressible Navier-Stokes equations for initial data around an equilibrium state.

  20. Analysis of lipid peroxidation kinetics. I

    DEFF Research Database (Denmark)

    Doktorov, Alexander B.; Lukzen, Nikita N.; Pedersen, Jørgen Boiden

    2008-01-01

      The kinetics of the lipid peroxidation reaction is only partly under-  stood. Although the set of reactions constituting the overall reaction  is believed to be known, it has not been possible to predict how the  reaction will respond to a change of one or more of the parameters, e.g.  initial......· ,  but not for the peroxyl radicals LO·2 as assumed in previous works.  The method allows us to derive manageable analytical expressions.  Based on literature values of the rate constants we are able to intro-  duce specific simplifications that allow us to obtain simple analytical  expressions for the time dependence of all...

  1. Friction analysis of kinetic schemes : the friction coefficient

    NARCIS (Netherlands)

    Lolkema, Juke S.

    1995-01-01

    Friction analysis is proposed as the application of general control analysis to single enzymes to describe the control of elementary kinetic steps on the overall catalytic rate. For each transition, a friction coefficient is defined that measures the sensitivity of the turnover rate to the free ener

  2. FRICTION ANALYSIS OF KINETIC SCHEMES - THE FRICTION COEFFICIENT

    NARCIS (Netherlands)

    LOLKEMA, JS

    1995-01-01

    Friction analysis is proposed as the application of general control analysis to single enzymes to describe the control of elementary kinetic steps on the overall catalytic rate. For each transition, a friction coefficient is defined that measures the sensitivity of the turnover rate to the free ener

  3. Friction analysis of kinetic schemes : the friction coefficient

    NARCIS (Netherlands)

    Lolkema, Juke S.

    1995-01-01

    Friction analysis is proposed as the application of general control analysis to single enzymes to describe the control of elementary kinetic steps on the overall catalytic rate. For each transition, a friction coefficient is defined that measures the sensitivity of the turnover rate to the free ener

  4. Pyrolysis kinetics of hazelnut husk using thermogravimetric analysis.

    Science.gov (United States)

    Ceylan, Selim; Topçu, Yıldıray

    2014-03-01

    This study aims at investigating physicochemical properties and pyrolysis kinetics of hazelnut husk, an abundant agricultural waste in Turkey. The physicochemical properties were determined by bomb calorimeter, elemental analysis and FT-IR spectroscopy. Physicochemical analysis results showed that hazelnut husk has a high calorimetric value and high volatile matter content. Pyrolysis experiments were carried out in a thermogravimetric analyzer under inert conditions and operated at different heating rates (5, 10, 20°C/min). Three different kinetic models, the iso-conversional Kissinger-Akahira-Sunose (KAS) and Ozawa-Flynn-Wall (OFW) models and Coats-Redfern method were applied on TGA data of hazelnut husk to calculate the kinetic parameters including activation energy, pre-exponential factor and reaction order. Simulation of hazelnut husk pyrolysis using data obtained from TGA analysis showed good agreement with experimental data. Combining with physicochemical properties, it was concluded that this biomass can become useful source of energy or chemicals.

  5. Development of the Kinetic Molecular Theory of Gases Concept Inventory: Preliminary Results on University Students' Misconceptions

    Science.gov (United States)

    Erceg, Nataša; Aviani, Ivica; Mešic, Vanes; Gluncic, Matko; Žauhar, Gordana

    2016-01-01

    In this study, we investigated students' understanding of concepts related to the microscopic model of gas. We thoroughly reviewed the relevant literature and conducted think alouds with students by asking them to answer open-ended questions about the kinetic molecular theory of gases. Thereafter, we transformed the open-ended questions into…

  6. Preliminary model and validation of molten carbonate fuel cell kinetics under sulphur poisoning

    Science.gov (United States)

    Audasso, E.; Nam, S.; Arato, E.; Bosio, B.

    2017-06-01

    MCFC represents an effective technology to deal with CO2 capture and relative applications. If used for these purposes, due to the working conditions and the possible feeding, MCFC must cope with a different number of poisoning gases such as sulphur compounds. In literature, different works deal with the development of kinetic models to describe MCFC performance to help both industrial applications and laboratory simulations. However, in literature attempts to realize a proper model able to consider the effects of poisoning compounds are scarce. The first aim of the present work is to provide a semi-empirical kinetic formulation capable to take into account the effects that sulphur compounds (in particular SO2) have on the MCFC performance. The second aim is to provide a practical example of how to effectively include the poisoning effects in kinetic models to simulate fuel cells performances. To test the reliability of the proposed approach, the obtained formulation is implemented in the kinetic core of the SIMFC (SIMulation of Fuel Cells) code, an MCFC 3D model realized by the Process Engineering Research Team (PERT) of the University of Genova. Validation is performed through data collected at the Korea Institute of Science and Technology in Seoul.

  7. Preliminary safety design analysis of KALIMER

    Energy Technology Data Exchange (ETDEWEB)

    Suk, Soo Dong; Kwon, Y. M.; Kim, K. D. [Korea Atomic Energy Research Institute, Taejon (Korea)

    1999-03-01

    The national long-term R and D program updated in 1997 requires Korea Atomic Energy Research Institute(KAERI) to complete by the year 2006 the basic design of Korea Advanced Liquid Metal Reactor (KALIMER), along with supporting R and D work, with the capability of resolving the issue of spent fuel storage as well as with significantly enhanced safety. KALIMER is a 150 MWe pool-type sodium cooled prototype reactor that uses metallic fuel. The conceptual design is currently under way to establish a self consistent design meeting a set of the major safety design requirements for accident prevention. Some of current emphasis include those for inherent and passive means of negative reactivity insertion and decay heat removal, high shutdown reliability, prevention of and protection from sodium chemical reaction, and high seismic margin, among others. All of these requirements affect the reactor design significantly and involve supporting R and D programs of substance. This document first introduces a set of safety design requirements and accident evaluation criteria established for the conceptual design of KALIMER and then summarizes some of the preliminary results of engineering and design analyses performed for the safety of KALIMER. 19 refs., 19 figs., 6 tabs. (Author)

  8. Preliminary analysis of patent trends for magnetic fusion technology

    Energy Technology Data Exchange (ETDEWEB)

    Levine, L.O.; Ashton, W.B.; Campbell, R.S.

    1984-02-01

    This study presents a preliminary analysis of development trends in magnetic fusion technology based on data from US patents. The research is limited to identification and description of general patent activity and ownership characteristics for 373 patents. The results suggest that more detailed studies of fusion patents could provide useful R and D planning information.

  9. Preliminary analysis of alternative fuel cycles for proliferation evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Steindler, M. J.; Ripfel, H. C.F.; Rainey, R. H.

    1977-01-01

    The ERDA Division of Nuclear Research and Applications proposed 67 nuclear fuel cycles for assessment as to their nonproliferation potential. The object of the assessment was to determine which fuel cycles pose inherently low risk for nuclear weapon proliferation while retaining the major benefits of nuclear energy. This report is a preliminary analysis of these fuel cycles to develop the fuel-recycle data that will complement reactor data, environmental data, and political considerations, which must be included in the overall evaluation. This report presents the preliminary evaluations from ANL, HEDL, ORNL, and SRL and is the basis for a continuing in-depth study. (DLC)

  10. A tool model for predicting atmospheric kinetics with sensitivity analysis

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A package( a tool model) for program of predicting atmospheric chemical kinetics with sensitivity analysis is presented. The new direct method of calculating the first order sensitivity coefficients using sparse matrix technology to chemical kinetics is included in the tool model, it is only necessary to triangularize the matrix related to the Jacobian matrix of the model equation. The Gear type procedure is used to integrate amodel equation and its coupled auxiliary sensitivity coefficient equations. The FORTRAN subroutines of the model equation, the sensitivity coefficient equations, and their Jacobian analytical expressions are generated automatically from a chemical mechanism. The kinetic representation for the model equation and its sensitivity coefficient equations, and their Jacobian matrix is presented. Various FORTRAN subroutines in packages, such as SLODE, modified MA28, Gear package, with which the program runs in conjunction are recommended.The photo-oxidation of dimethyl disulfide is used for illustration.

  11. Preliminary Integrated Safety Analysis Status Report

    Energy Technology Data Exchange (ETDEWEB)

    D. Gwyn

    2001-04-01

    This report provides the status of the potential Monitored Geologic Repository (MGR) Integrated Safety Analysis (EA) by identifying the initial work scope scheduled for completion during the ISA development period, the schedules associated with the tasks identified, safety analysis issues encountered, and a summary of accomplishments during the reporting period. This status covers the period from October 1, 2000 through March 30, 2001.

  12. Kinetic analysis of complex metabolic networks

    Energy Technology Data Exchange (ETDEWEB)

    Stephanopoulos, G. [MIT, Cambridge, MA (United States)

    1996-12-31

    A new methodology is presented for the analysis of complex metabolic networks with the goal of metabolite overproduction. The objective is to locate a small number of reaction steps in a network that have maximum impact on network flux amplification and whose rate can also be increased without functional network derangement. This method extends the concepts of Metabolic Control Analysis to groups of reactions and offers the means for calculating group control coefficients as measures of the control exercised by groups of reactions on the overall network fluxes and intracellular metabolite pools. It is further demonstrated that the optimal strategy for the effective increase of network fluxes, while maintaining an uninterrupted supply of intermediate metabolites, is through the coordinated amplification of multiple (as opposed to a single) reaction steps. Satisfying this requirement invokes the concept of the concentration control to coefficient, which emerges as a critical parameter in the identification of feasible enzymatic modifications with maximal impact on the network flux. A case study of aromatic aminoacid production is provided to illustrate these concepts.

  13. Fort Drum Preliminary Fiscal Impact Analysis.

    Science.gov (United States)

    1986-02-01

    of inmigrants 0 Fiscal histories, projections, and impacts for counties, cities, towns, villages, school districts, and the state. The results of...distribution of the inmigrating population within the three counties. Thus, an accurate forecast of the expected distribution of the inmigrating population is a...The distribution of inmigration to the school districts was made using the analysis explained in Chapter 3. Children associated with 800 new on-post

  14. Development of the kinetic molecular theory of gases concept inventory: Preliminary results on university students’ misconceptions

    Directory of Open Access Journals (Sweden)

    Nataša Erceg

    2016-11-01

    Full Text Available In this study, we investigated students’ understanding of concepts related to the microscopic model of gas. We thoroughly reviewed the relevant literature and conducted think alouds with students by asking them to answer open-ended questions about the kinetic molecular theory of gases. Thereafter, we transformed the open-ended questions into multiple-choice questions, whereby distractors were based on the results of the think alouds. Thus, we obtained a set of 22 questions, which constitutes our current version of the kinetic molecular theory of gases concept inventory. The inventory has been administered to 250 students from different universities in Croatia, and its content validity has been investigated trough physics teacher surveys. The results of our study not only corroborate the existence of some already known student misconceptions, but also reveal new insights about a great spectrum of students’ misconceptions that had not been reported in earlier research (e.g., misconceptions about intermolecular potential energy and molecular velocity distribution. Moreover, we identified similar distribution of students’ responses across the surveyed student groups, despite the fact that they had been enrolled in different curricular environments.

  15. Preliminary study: kinetics of oil extraction from sandalwood by microwave-assisted hydrodistillation

    Science.gov (United States)

    Kusuma, H. S.; Mahfud, M.

    2016-04-01

    Sandalwood and its oil, is one of the oldest known perfume materials and has a long history (more than 4000 years) of use as mentioned in Sanskrit manuscripts. Sandalwood oil plays an important role as an export commodity in many countries and its widely used in the food, perfumery and pharmaceuticals industries. The aim of this study is to know and verify the kinetics and mechanism of microwave-assisted hydrodistillation of sandalwood based on a second-order model. In this study, microwave-assisted hydrodistillation is used to extract essential oils from sandalwood. The extraction was carried out in ten extraction cycles of 15 min to 2.5 hours. The initial extraction rate, the extraction capacity and the second-order extraction rate constant were calculated using the model. Kinetics of oil extraction from sandalwood by microwave-assisted hydrodistillation proved that the extraction process was based on the second-order extraction model as the experimentally done in three different steps. The initial extraction rate, h, was 0.0232 g L-1 min-1, the extraction capacity, C S, was 0.6015 g L-1, the second-order extraction rate constant, k, was 0.0642 L g-1 min-1 and coefficient of determination, R 2, was 0.9597.

  16. Progress Toward the Analysis of the Kinetic Stabilizer Concept

    Energy Technology Data Exchange (ETDEWEB)

    Post, R F; Byers, J A; Cohen, R H; Fowler, T K; Ryutov, D D; Tung, L S

    2005-02-08

    The Kinetic Stabilizer (K-S) concept [1] represents a means for stabilizing axisymmetric mirror and tandem-mirror (T-M) magnetic fusion systems against MHD interchange instability modes. Magnetic fusion research has given us examples of axisymmetric mirror confinement devices in which radial transport rates approach the classical ''Spitzer'' level, i.e. situations in which turbulence if present at all, is at too low a level to adversely affect the radial transport [2,3,4]. If such a low-turbulence condition could be achieved in a T-M system it could lead to a fusion power system that would be simpler, smaller, and easier to develop than one based on closed-field confinement, e.g., the tokamak, where the transport is known to be dominated by turbulence. However, since conventional axisymmetric mirror systems suffer from the MHD interchange instability, the key to exploiting this new opportunity is to find a practical way to stabilize this mode. The K-S represents one avenue to achieving this goal. The starting point for the K-S concept is a theoretical analysis by Ryutov [5]. He showed that a MHD-unstable plasma contained in an axisymmetric mirror cell can be MHD-stabilized by the presence of a low-density plasma on the expanding field lines outside the mirrors. If this plasma communicates well electrically with the plasma in the then this exterior plasma can stabilize the interior, confined, plasma. This stabilization technique was conclusively demonstrated in the Gas Dynamic Trap (GDT) experiment [6] at Novosibirsk, Russia, at mirror-cell plasma beta values of 40 percent. The GDT operates in a high collisionality regime. Thus the effluent plasma leaking through the mirrors, though much lower in density than that of the confined plasma, is still high enough to satisfy the stabilization criterion. This would not, however, be the case in a fusion T-M with axisymmetric plug and central cell fields. In such a case the effluent plasma would be far

  17. Preliminary analysis of turbochargers rotors dynamic behaviour

    Science.gov (United States)

    Monoranu, R.; Ştirbu, C.; Bujoreanu, C.

    2016-08-01

    Turbocharger rotors for the spark and compression ignition engines are resistant steels manufactured in order to support the exhaust gas temperatures exceeding 1200 K. In fact, the mechanical stress is not large as the power consumption of these systems is up to 10 kW, but the operating speeds are high, ranging between 30000 ÷ 250000 rpm. Therefore, the correct turbochargers functioning involves, even from the design stage, the accurate evaluation of the temperature effects, of the turbine torque due to the engine exhaust gases and of the vibration system behaviour caused by very high operating speeds. In addition, the turbocharger lubrication complicates the model, because the classical hydrodynamic theory cannot be applied to evaluate the floating bush bearings. The paper proposes a FEM study using CATIA environment, both as modeling medium and as tool for the numerical analysis, in order to highlight the turbocharger complex behaviour. An accurate design may prevent some major issues which can occur during its operation.

  18. Mechanism and kinetics analysis of AlN combustion synthesis

    Institute of Scientific and Technical Information of China (English)

    郑永挺; 张宇民; 赫晓东; 刘长青

    2004-01-01

    Mechanism of AlN combustion synthesis was studied by DSC analysis and "quenching" experiment of combustion wave. In preheating region, Al powder melted at 660 ℃ and became spherical because of surface tension. In reaction region, Al volatilized rapidly at 1 000 ℃ and Al vapor reacted with N2 to form AlN, which nucleated and grew on the surface of AlN formed precedently as a diluent. Based on the experiment analysis,kinetics equations and modeling were established. Analysis showed the dramatic effect of temperature, aluminum particle size and nitrogen pressure on the reaction speed.

  19. Preliminary Analysis of Helicopter Options to Support Tunisian Counterterrorism Operations

    Science.gov (United States)

    2016-04-27

    results of the current analysis and in Mouton et al., 2015, is the relative cost -effectiveness between the CH-47D and the Mi-17v5. In the previous...helicopters from Sikorsky to fulfill a number of roles in counterterrorism operations. Rising costs and delays in delivery raised the question of...whether other cost -effective options exist to meet Tunisia’s helicopter requirement. Approach Our team conducted a preliminary assessment of

  20. LSENS - GENERAL CHEMICAL KINETICS AND SENSITIVITY ANALYSIS CODE

    Science.gov (United States)

    Bittker, D. A.

    1994-01-01

    LSENS has been developed for solving complex, homogeneous, gas-phase, chemical kinetics problems. The motivation for the development of this program is the continuing interest in developing detailed chemical reaction mechanisms for complex reactions such as the combustion of fuels and pollutant formation and destruction. A reaction mechanism is the set of all elementary chemical reactions that are required to describe the process of interest. Mathematical descriptions of chemical kinetics problems constitute sets of coupled, nonlinear, first-order ordinary differential equations (ODEs). The number of ODEs can be very large because of the numerous chemical species involved in the reaction mechanism. Further complicating the situation are the many simultaneous reactions needed to describe the chemical kinetics of practical fuels. For example, the mechanism describing the oxidation of the simplest hydrocarbon fuel, methane, involves over 25 species participating in nearly 100 elementary reaction steps. Validating a chemical reaction mechanism requires repetitive solutions of the governing ODEs for a variety of reaction conditions. Analytical solutions to the systems of ODEs describing chemistry are not possible, except for the simplest cases, which are of little or no practical value. Consequently, there is a need for fast and reliable numerical solution techniques for chemical kinetics problems. In addition to solving the ODEs describing chemical kinetics, it is often necessary to know what effects variations in either initial condition values or chemical reaction mechanism parameters have on the solution. Such a need arises in the development of reaction mechanisms from experimental data. The rate coefficients are often not known with great precision and in general, the experimental data are not sufficiently detailed to accurately estimate the rate coefficient parameters. The development of a reaction mechanism is facilitated by a systematic sensitivity analysis

  1. Kinetic analysis of the multistep aggregation mechanism of monoclonal antibodies.

    Science.gov (United States)

    Nicoud, Lucrèce; Arosio, Paolo; Sozo, Margaux; Yates, Andrew; Norrant, Edith; Morbidelli, Massimo

    2014-09-11

    We investigate by kinetic analysis the aggregation mechanism of two monoclonal antibodies belonging to the IgG1 and IgG2 subclass under thermal stress. For each IgG, we apply a combination of size exclusion chromatography and light scattering techniques to resolve the time evolution of the monomer, dimer, and trimer concentrations, as well as the average molecular weight and the average hydrodynamic radius of the aggregate distribution. By combining the detailed experimental characterization with a theoretical kinetic model based on population balance equations, we extract relevant information on the contribution of the individual elementary steps on the global aggregation process. The analysis shows that the two molecules follow different aggregation pathways under the same operating conditions. In particular, while the monomer depletion of the IgG1 is found to be rate-limited by monomeric conformational changes, bimolecular collision is identified as the rate-limiting step in the IgG2 aggregation process. The measurement of the microscopic rate constants by kinetic analysis allows the quantification of the protein-protein interaction potentials expressed in terms of the Fuchs stability ratio (W). It is found that the antibody solutions exhibit large W values, which are several orders of magnitude larger than the values computed in the frame of the DLVO theory. This indicates that, besides net electrostatic repulsion, additional effects delay the aggregation kinetics of the antibody solutions with respect to diffusion-limited conditions. These effects likely include the limited efficiency of the collision events due to the presence of a limited number of specific aggregation-prone patches on the heterogeneous protein surface, and the contribution of additional repulsive non-DLVO forces to the protein-protein interaction potential, such as hydration forces.

  2. Comparative kinetic analysis of two fungal β-glucosidases

    Directory of Open Access Journals (Sweden)

    Casanave Dominique

    2010-02-01

    Full Text Available Abstract Background The enzymatic hydrolysis of cellulose is still considered as one of the main limiting steps of the biological production of biofuels from lignocellulosic biomass. It is a complex multistep process, and various kinetic models have been proposed. The cellulase enzymatic cocktail secreted by Trichoderma reesei has been intensively investigated. β-glucosidases are one of a number of cellulolytic enzymes, and catalyze the last step releasing glucose from the inhibitory cellobiose. β-glucosidase (BGL1 is very poorly secreted by Trichoderma reesei strains, and complete hydrolysis of cellulose often requires supplementation with a commercial β-glucosidase preparation such as that from Aspergillus niger (Novozymes SP188. Surprisingly, kinetic modeling of β-glucosidases lacks reliable data, and the possible differences between native T. reesei and supplemented β-glucosidases are not taken into consideration, possibly because of the difficulty of purifying BGL1. Results A comparative kinetic analysis of β-glucosidase from Aspergillus niger and BGL1 from Trichoderma reesei, purified using a new and efficient fast protein liquid chromatography protocol, was performed. This purification is characterized by two major steps, including the adsorption of the major cellulases onto crystalline cellulose, and a final purification factor of 53. Quantitative analysis of the resulting β-glucosidase fraction from T. reesei showed it to be 95% pure. Kinetic parameters were determined using cellobiose and a chromogenic artificial substrate. A new method allowing easy and rapid determination of the kinetic parameters was also developed. β-Glucosidase SP188 (Km = 0.57 mM; Kp = 2.70 mM has a lower specific activity than BGL1 (Km = 0.38 mM; Kp = 3.25 mM and is also more sensitive to glucose inhibition. A Michaelis-Menten model integrating competitive inhibition by the product (glucose has been validated and is able to predict the

  3. Kinetic analysis of two dimensional metallic grating Cerenkov maser

    Energy Technology Data Exchange (ETDEWEB)

    Zhao Ding [Key Laboratory of High Power Microwave Sources and Technologies, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190 (China)

    2011-08-15

    The dispersion relation of two dimensional metallic grating Cerenkov maser has been given by using kinetic analysis, in which the influence of electron movement is directly considered without using an equivalent dielectric medium assumption. The effects of structural parameters and beam state on the interaction gain and synchronous frequency have also been investigated in detail by numerical calculations. To an illustrative case, the quantitative relations produced from varying the gap distance between electron beam and metallic grating, beam current, electron transverse to axial velocity ratio, and electron axial velocity spread have been obtained. The developed method can be used to predict the real interaction system performances.

  4. A uranium (VI) complex: Synthesis, structural and thermal kinetic analysis

    Science.gov (United States)

    Goel, Nidhi

    2016-08-01

    A new complex [UO2(2,6-DNP)2phen] (1) (2,6-DNP = 2,6-dinitrophenol, phen = 1,10-phenanthroline) was synthesized, and identified by elemental analysis, IR, Powder XRD and single crystal X-ray crystallography. Crystal structure provides the abundant information's about the bonding and geometry around the U(VI) metal center. The thermal decomposition was studied by TG-DSC, and the kinetics of thermolysis was investigated by applying model fitting as well as isoconversional methods. Explosion delay measurement (De) was also evaluated to determine the response of this complex under the condition of rapid heating.

  5. NRT Rotor Structural / Aeroelastic Analysis for the Preliminary Design Review

    Energy Technology Data Exchange (ETDEWEB)

    Ennis, Brandon Lee [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Paquette, Joshua A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-10-01

    This document describes the initial structural design for the National Rotor Testbed blade as presented during the preliminary design review at Sandia National Laboratories on October 28- 29, 2015. The document summarizes the structural and aeroelastic requirements placed on the NRT rotor for satisfactory deployment at the DOE/SNL SWiFT experimental facility to produce high-quality datasets for wind turbine model validation. The method and result of the NRT blade structural optimization is also presented within this report, along with analysis of its satisfaction of the design requirements.

  6. Preliminary Analysis of ULPC Light Curves Using Fourier Decomposition Technique

    CERN Document Server

    Ngeow, Chow-Choong; Kanbur, Shashi; Barrett, Brittany; Lin, Bin

    2013-01-01

    Recent work on Ultra Long Period Cepheids (ULPCs) has suggested their usefulness as a distance indicator, but has not commented on their relationship as compared with other types of variable stars. In this work, we use Fourier analysis to quantify the structure of ULPC light curves and compare them to Classical Cepheids and Mira variables. Our preliminary results suggest that the low order Fourier parameters of ULPCs show a continuous trend defined by Classical Cepheids after the resonance around 10 days. However their Fourier parameters also overlapped with those from Miras, which make the classification of long period variable stars difficult based on the light curves information alone.

  7. Determinants of Trade Credit: A Preliminary Analysis on Construction Sector

    Directory of Open Access Journals (Sweden)

    Nicoleta Barbuta-Misu

    2016-07-01

    Full Text Available This paper introduces a preliminary analysis of the correlations between trade credit and some selected measures of financial performance for a sample of 958 firms acting in the construction sector. The examined period covers 2004-2013. The sample derived from Amadeus database contains firms that have sold and bought on credit. Results showed that larger firms offered and used more credit than counterparties. Firms offered and used in same time credit, but not in same level. Firms with higher return on assets and profit margin used and offered less credit from suppliers, respectively to clients. Moreover, more liquid firms used less trade payables.

  8. CONTENT ANALYSIS, DISCOURSE ANALYSIS, AND CONVERSATION ANALYSIS: PRELIMINARY STUDY ON CONCEPTUAL AND THEORETICAL METHODOLOGICAL DIFFERENCES

    Directory of Open Access Journals (Sweden)

    Anderson Tiago Peixoto Gonçalves

    2016-08-01

    Full Text Available This theoretical essay aims to reflect on three models of text interpretation used in qualitative research, which is often confused in its concepts and methodologies (Content Analysis, Discourse Analysis, and Conversation Analysis. After the presentation of the concepts, the essay proposes a preliminary discussion on conceptual and theoretical methodological differences perceived between them. A review of the literature was performed to support the conceptual and theoretical methodological discussion. It could be verified that the models have differences related to the type of strategy used in the treatment of texts, the type of approach, and the appropriate theoretical position.

  9. Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis

    Science.gov (United States)

    Liu, Zhi-Wei; Tong, Zhao-Yang; Liu, Bing; Hao, Lan-Qun; Mu, Xi-Hui; Zhang, Jin-Ping; Gao, Chuan

    2015-04-01

    Up to now, there has been no report on target molecules detection by a piezoresistive microcantilever aptasensor. In order to evaluate the test performance and investigate the response dynamic characteristics of a piezoresistive microcantilever aptasensor, a novel method for ricin detection and kinetic analysis based on a piezoresistive microcantilever aptasensor was proposed, where ricin aptamer was immobilised on the microcantilever surface by biotin-avidin binding system. Results showed that the detection limit of ricin was 0.04μg L-1 (S/N ≥ 3). A linear relationship between the response voltage and the concentration of ricin in the range of 0.2μg L-1-40μg L-1 was obtained, with the linear regression equation of ΔUe = 0.904C + 5.852 (n = 5, R = 0.991, p soil, and flour sample) determination met the analysis requirements, which was 90.5˜95.5% and 7.85%˜9.39%, respectively. On this basis, a reaction kinetic model based on ligand-receptor binding and the relationship with response voltage was established. The model could well reflect the dynamic response of the sensor. The correlation coefficient (R) was greater than or equal to 0.9456 (p < 0.001). Response voltage (ΔUe) and response time (t0) obtained from the fitting equation on different concentrations of ricin fitted well with the measured values.

  10. Protein folding: Defining a standard set of experimental conditions and a preliminary kinetic data set of two-state proteins

    DEFF Research Database (Denmark)

    Maxwell, Karen L.; Wildes, D.; Zarrine-Afsar, A.;

    2005-01-01

    constructs. The lack of a single approach to data analysis and error estimation, or even of a common set of units and reporting standards, further hinders comparative studies of folding. In an effort to overcome these problems, we define here a consensus set of experimental conditions (25°C at pH 7.0, 50 m...... rates, thermodynamics, and structure across diverse sets of proteins. These difficulties include the wide, potentially confounding range of experimental conditions and methods employed to date and the difficulty of obtaining correct and complete sequence and structural details for the characterized......M buffer), data analysis methods, and data reporting standards that we hope will provide a benchmark for experimental studies. We take the first step in this initiative by describing the folding kinetics of 30 apparently two-state proteins or protein domains under the consensus conditions. The goal of our...

  11. Enhanced Accident Tolerant Fuels for LWRS - A Preliminary Systems Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Gilles Youinou; R. Sonat Sen

    2013-09-01

    The severe accident at Fukushima Daiichi nuclear plants illustrates the need for continuous improvements through developing and implementing technologies that contribute to safe, reliable and cost-effective operation of the nuclear fleet. Development of enhanced accident tolerant fuel contributes to this effort. These fuels, in comparison with the standard zircaloy – UO2 system currently used by the LWR industry, should be designed such that they tolerate loss of active cooling in the core for a longer time period (depending on the LWR system and accident scenario) while maintaining or improving the fuel performance during normal operations, operational transients, and design-basis events. This report presents a preliminary systems analysis related to most of these concepts. The potential impacts of these innovative LWR fuels on the front-end of the fuel cycle, on the reactor operation and on the back-end of the fuel cycle are succinctly described without having the pretension of being exhaustive. Since the design of these various concepts is still a work in progress, this analysis can only be preliminary and could be updated as the designs converge on their respective final version.

  12. Electronic Warfare M-on-N Digital Simulation Logging Requirements and HDF5: A Preliminary Analysis

    Science.gov (United States)

    2017-04-12

    E. Jarvis Electronic Warfare M-on- N Digital Simulation Logging Requirements and HDF5: A Preliminary Analysis Advanced Techniques Branch Tactical...12-04-2017 NRL Memorandum Report Electronic Warfare M-on- N Digital Simulation Logging Requirements and HDF5: A Preliminary Analysis Donald E...ELECTRONIC WARFARE M-ON- N DIGITAL SIMULATION LOGGING REQUIREMENTS AND HDF5: A PRELIMINARY ANALYSIS 1. INTRODUCTION HDF5 technology [Folk] has been

  13. Preliminary hazards analysis of thermal scrap stabilization system. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, W.S.

    1994-08-23

    This preliminary analysis examined the HA-21I glovebox and its supporting systems for potential process hazards. Upon further analysis, the thermal stabilization system has been installed in gloveboxes HC-21A and HC-21C. The use of HC-21C and HC-21A simplified the initial safety analysis. In addition, these gloveboxes were cleaner and required less modification for operation than glovebox HA-21I. While this document refers to glovebox HA-21I for the hazards analysis performed, glovebox HC-21C is sufficiently similar that the following analysis is also valid for HC-21C. This hazards analysis document is being re-released as revision 1 to include the updated flowsheet document (Appendix C) and the updated design basis (Appendix D). The revised Process Flow Schematic has also been included (Appendix E). This Current revision incorporates the recommendations provided from the original hazards analysis as well. The System Design Description (SDD) has also been appended (Appendix H) to document the bases for Safety Classification of thermal stabilization equipment.

  14. Analysis preliminary phytochemical raw extract of leaves Nephrolepis pectinata

    Directory of Open Access Journals (Sweden)

    Natally Marreiros Gomes

    2017-06-01

    Full Text Available The Nephrolepis pectinata popularly known as paulista fern, ladder-heaven, cat tail, belongs to the family Davalliaceae. For the beauty of the arrangements of their leaves ferns are quite commercialized in Brazil, however, have not been described in the literature studies on their pharmacological potential. Thus, the objective of this research was to analyze the phytochemical properties of the crude extract of the leaves of Nephrolepis pectinata. To perform the phytochemical analysis were initially made the collection of the vegetable, preparation of voucher specimen, washing, drying and grinding. Then, extraction by percolation method and end the phytochemical analysis. Preliminary results phytochemicals the crude extract of the leaves of Nephrolepis pectinata tested positive for reducing sugars, phenols/tannins (catechins tannins and catechins.

  15. Kinetic Gait Analysis Using a Low-Cost Insole.

    Science.gov (United States)

    Howell, Adam M; Kobayashi, Toshiki; Hayes, Heather A; Foreman, K Bo; Bamberg, Stacy J Morris

    2013-12-01

    Abnormal gait caused by stroke or other pathological reasons can greatly impact the life of an individual. Being able to measure and analyze that gait is often critical for rehabilitation. Motion analysis labs and many current methods of gait analysis are expensive and inaccessible to most individuals. The low-cost, wearable, and wireless insole-based gait analysis system in this study provides kinetic measurements of gait by using low-cost force sensitive resistors. This paper describes the design and fabrication of the insole and its evaluation in six control subjects and four hemiplegic stroke subjects. Subject-specific linear regression models were used to determine ground reaction force plus moments corresponding to ankle dorsiflexion/plantarflexion, knee flexion/extension, and knee abduction/adduction. Comparison with data simultaneously collected from a clinical motion analysis laboratory demonstrated that the insole results for ground reaction force and ankle moment were highly correlated (all >0.95) for all subjects, while the two knee moments were less strongly correlated (generally >0.80). This provides a means of cost-effective and efficient healthcare delivery of mobile gait analysis that can be used anywhere from large clinics to an individual's home.

  16. Ultrasonic effects on the degradation kinetics, preliminary characterization and antioxidant activities of polysaccharides from Phellinus linteus mycelia.

    Science.gov (United States)

    Yan, Jing-Kun; Wang, Yao-Yao; Ma, Hai-Le; Wang, Zhen-Bin

    2016-03-01

    In this study, a high-molecular-weight polysaccharide PL-N isolated from the alkaline extract of Phellinus linteus mycelia was degraded by ultrasound. Results showed that ultrasound treatment at different ultrasonic intensities decreased the intrinsic viscosity and molecular weight of PL-N, as well as narrowed the molecular weight distribution. A larger reduction in intrinsic viscosity and molecular weight was caused by a higher ultrasonic intensity. The degradation kinetics model was fitted to (1/Mt-1/M0)=k·t, and the reaction rate constant (k) increased with increasing ultrasonic intensity. Ultrasound degradation did not change the primary structure of PL-N, and scanning electron microscopy analysis indicated that the morphology of the original PL-N was different from that of degraded PL-N fractions. Antioxidant activity assays in vitro indicated that the degraded PL-N fraction with low molecular weight had stronger hydroxyl radical scavenging capacity and higher TEAC and FRAP values.

  17. [Efficient method of analysis of optical spectra from kinetic studies].

    Science.gov (United States)

    Skvortsov, A N

    2009-01-01

    The application of principal components for the analysis of kinetic data obtained by optical spectroscopy is described. The use of singular value decomposition (SVD) for stable and reproducible generation of principal components, details of realization, advantages and drawbacks of the method are discussed. The described method with minor modifications may be used in a wide variety of UV-spectroscopy applications in molecular biology and biophysics. The developed method was applied to study the reaction of platinum anticancer drug, cisplatin, with DNA and methionine. Use of sensitive UV-spectroscopy allowed to study low platinum concentrations, typical for biological systems. It has been shown, that reactions of cisplatin with DNA and L-methionine generally follow the same pathway both at high and low concentrations.

  18. Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis

    Directory of Open Access Journals (Sweden)

    Zhi-Wei Liu

    2015-04-01

    Full Text Available Up to now, there has been no report on target molecules detection by a piezoresistive microcantilever aptasensor. In order to evaluate the test performance and investigate the response dynamic characteristics of a piezoresistive microcantilever aptasensor, a novel method for ricin detection and kinetic analysis based on a piezoresistive microcantilever aptasensor was proposed, where ricin aptamer was immobilised on the microcantilever surface by biotin-avidin binding system. Results showed that the detection limit of ricin was 0.04μg L−1 (S/N ≥ 3. A linear relationship between the response voltage and the concentration of ricin in the range of 0.2μg L−1-40μg L−1 was obtained, with the linear regression equation of ΔUe = 0.904C + 5.852 (n = 5, R = 0.991, p < 0.001. The sensor showed no response for abrin, BSA, and could overcome the influence of complex environmental disruptors, indicating high specificity and good selectivity. Recovery and reproducibility in the result of simulated samples (simulated water, soil, and flour sample determination met the analysis requirements, which was 90.5∼95.5% and 7.85%∼9.39%, respectively. On this basis, a reaction kinetic model based on ligand-receptor binding and the relationship with response voltage was established. The model could well reflect the dynamic response of the sensor. The correlation coefficient (R was greater than or equal to 0.9456 (p < 0.001. Response voltage (ΔUe and response time (t0 obtained from the fitting equation on different concentrations of ricin fitted well with the measured values.

  19. Solar Stirling power generation - Systems analysis and preliminary tests

    Science.gov (United States)

    Selcuk, M. K.; Wu, Y.-C.; Moynihan, P. I.; Day, F. D., III

    1977-01-01

    The feasibility of an electric power generation system utilizing a sun-tracking parabolic concentrator and a Stirling engine/linear alternator is being evaluated. Performance predictions and cost analysis of a proposed large distributed system are discussed. Design details and preliminary test results are presented for a 9.5 ft diameter parabolic dish at the Jet Propulsion Laboratory (Caltech) Table Mountain Test Facility. Low temperature calorimetric measurements were conducted to evaluate the concentrator performance, and a helium flow system is being used to test the solar receiver at anticipated working fluid temperatures (up to 650 or 1200 C) to evaluate the receiver thermal performance. The receiver body is designed to adapt to a free-piston Stirling engine which powers a linear alternator assembly for direct electric power generation. During the next phase of the program, experiments with an engine and receiver integrated into the concentrator assembly are planned.

  20. Primate phylogeny studied by comparative determinant analysis. A preliminary report.

    Science.gov (United States)

    Bauer, K

    1993-01-01

    In this preliminary report the divergence times for the major primate groups are given, calculated from a study by comparative determinant analysis of 69 proteins (equaling 0.1% of the whole genetic information). With an origin of the primate order set at 80 million years before present, the ages of the last common ancestors (LCAs) of man and the major primate groups obtained this way are as follows: Pan troglodytes 5.2; Gorilla gorilla 7.4; Pongo pygmaeus 19.2; Hylobates lar 20.3; Old World monkeys 31.4; Lagothrix lagotricha 46.0; Cebus albifrons 59.5; three lemur species 67.0, and Galago crassicaudatus 73.3 million years. The LCA results and the approach are shortly discussed. A full account of this extended investigation including results on nonprimate mammals and on the determinant structures and the immunologically derived evolutionary rates of the proteins analyzed will be published elsewhere.

  1. PRELIMINARY PHYTOCHEMICAL ANALYSIS OF ACTINIOPTERIS RADIATA (SWARTZ LINK.

    Directory of Open Access Journals (Sweden)

    R. Manonmani

    2013-06-01

    Full Text Available The objective of the present study was to find out the presence of preliminary phytochemicals in six different solvent extracts of Actiniopteris radiata (Swartz link. by qualitative screening methods. The solvent used for the extraction of leaf and rhizome powder were ethanol, petroleum ether, chloroform, acetone, DMSO and aqueous. The secondary metabolites such as steroids, triterpenoids, reducing sugars, sugars, alkaloids, phenolic compounds, catechins, flavonoids, saponins, tannins, anthroquinones and amino acids were screened by using standard methods. The phytochemical analysis of the ethanolic extract of both (leaf & rhizome revealed the presence of most active constituents than the other solvents. The ethanolic rhizome extracts of Actiniopteris radiata showed higher amount of phytochemicals when compared with the ethanolic leaf extracts.

  2. CUSUM control charts based on likelihood ratio for preliminary analysis

    Institute of Scientific and Technical Information of China (English)

    Yi DAI; Zhao-jun WANG; Chang-liang ZOU

    2007-01-01

    To detect and estimate a shift in either the mean and the deviation or both for the preliminary analysis, the statistical process control (SPC) tool, the control chart based on the likelihood ratio test (LRT), is the most popular method.Sullivan and woodall pointed out the test statistic lrt (n1, n2) is approximately distributed as x2 (2) as the sample size n, n1 and n2 are very large, and the value of n1 = 2, 3,..., n- 2 and that of n2 = n- n1.So it is inevitable that n1 or n2 is not large. In this paper the limit distribution of lrt(n1, n2) for fixed n1 or n2 is figured out, and the exactly analytic formulae for evaluating the expectation and the variance of the limit distribution are also obtained.In addition, the properties of the standardized likelihood ratio statistic slr(n1,n) are discussed in this paper. Although slr(n1, n) contains the most important information, slr(i, n)(i ≠ n1) also contains lots of information. The cumulative sum (CUSUM) control chart can obtain more information in this condition. So we propose two CUSUM control charts based on the likelihood ratio statistics for the preliminary analysis on the individual observations. One focuses on detecting the shifts in location in the historical data and the other is more general in detecting a shift in either the location and the scale or both.Moreover, the simulated results show that the proposed two control charts are, respectively, superior to their competitors not only in the detection of the sustained shifts but also in the detection of some other out-of-control situations considered in this paper.

  3. CUSUM control charts based on likelihood ratio for preliminary analysis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    To detect and estimate a shift in either the mean and the deviation or both for the preliminary analysis, the statistical process control (SPC) tool, the control chart based on the likelihood ratio test (LRT), is the most popular method. Sullivan and woodall pointed out the test statistic lrt(n1, n2) is approximately distributed as x2(2) as the sample size n,n1 and n2 are very large, and the value of n1 = 2,3,..., n - 2 and that of n2 = n - n1. So it is inevitable that n1 or n2 is not large. In this paper the limit distribution of lrt(n1, n2) for fixed n1 or n2 is figured out, and the exactly analytic formulae for evaluating the expectation and the variance of the limit distribution are also obtained. In addition, the properties of the standardized likelihood ratio statistic slr(n1, n) are discussed in this paper. Although slr(n1, n) contains the most important information, slr(i, n)(i≠n1) also contains lots of information. The cumulative sum (CUSUM) control chart can obtain more information in this condition. So we propose two CUSUM control charts based on the likelihood ratio statistics for the preliminary analysis on the individual observations. One focuses on detecting the shifts in location in the historical data and the other is more general in detecting a shift in either the location and the scale or both. Moreover, the simulated results show that the proposed two control charts are, respectively, superior to their competitors not only in the detection of the sustained shifts but also in the detection of some other out-of-control situations considered in this paper.

  4. Kinetic analysis of overlapping multistep thermal decomposition comprising exothermic and endothermic processes: thermolysis of ammonium dinitramide.

    Science.gov (United States)

    Muravyev, Nikita V; Koga, Nobuyoshi; Meerov, Dmitry B; Pivkina, Alla N

    2017-01-25

    This study focused on kinetic modeling of a specific type of multistep heterogeneous reaction comprising exothermic and endothermic reaction steps, as exemplified by the practical kinetic analysis of the experimental kinetic curves for the thermal decomposition of molten ammonium dinitramide (ADN). It is known that the thermal decomposition of ADN occurs as a consecutive two step mass-loss process comprising the decomposition of ADN and subsequent evaporation/decomposition of in situ generated ammonium nitrate. These reaction steps provide exothermic and endothermic contributions, respectively, to the overall thermal effect. The overall reaction process was deconvoluted into two reaction steps using simultaneously recorded thermogravimetry and differential scanning calorimetry (TG-DSC) curves by considering the different physical meanings of the kinetic data derived from TG and DSC by P value analysis. The kinetic data thus separated into exothermic and endothermic reaction steps were kinetically characterized using kinetic computation methods including isoconversional method, combined kinetic analysis, and master plot method. The overall kinetic behavior was reproduced as the sum of the kinetic equations for each reaction step considering the contributions to the rate data derived from TG and DSC. During reproduction of the kinetic behavior, the kinetic parameters and contributions of each reaction step were optimized using kinetic deconvolution analysis. As a result, the thermal decomposition of ADN was successfully modeled as partially overlapping exothermic and endothermic reaction steps. The logic of the kinetic modeling was critically examined, and the practical usefulness of phenomenological modeling for the thermal decomposition of ADN was illustrated to demonstrate the validity of the methodology and its applicability to similar complex reaction processes.

  5. Analysis of interface kinetics: solutions of the Gibbs-Thomson-type equation and of the kinetic rate theory

    Science.gov (United States)

    Salhoumi, A.; Galenko, P. K.

    2017-04-01

    Rapidly moving solid-liquid interface is treated analytically and numerically. Derivation and qualitative analysis of interface propagation kinetics is presented. Quantitative predictions of solutions, which follow from the Kinetic Rate Theory and the solution of Gibbs-Thomson-type equation, are compared with Molecular Dynamics simulation data (MD-data) on crystallization and melting of fcc-lattice of nickel. It is shown in the approximation of a linear behavior of the interface velocity versus undercooling that the Gibbs-Thomson-type equation and kinetic rate theory describe MD-data well enough, in the range of small growth velocity and within the range of relatively small undercooling, with a relative error for the obtained values of kinetic coefficient of the order 1.1%. Within the small-and long range of undercooling, in nonlinear behavior of the interface velocity versus undercooling, the kinetic rate theory disagrees sharply with MD-data, qualitatively and quantitatively, unlike to the Gibbs-Thomson-type equation which is in a good agreement with MD-data within the whole range of undercooling and crystal growth velocity.

  6. Kinetic analysis of manure pyrolysis and combustion processes.

    Science.gov (United States)

    Fernandez-Lopez, M; Pedrosa-Castro, G J; Valverde, J L; Sanchez-Silva, L

    2016-12-01

    Due to the depletion of fossil fuel reserves and the environmental issues derived from their use, biomass seems to be an excellent source of renewable energy. In this work, the kinetics of the pyrolysis and combustion of three different biomass waste samples (two dairy manure samples before (Pre) and after (Dig R) anaerobic digestion and one swine manure sample (SW)) was studied by means of thermogravimetric analysis. In this work, three iso-conversional methods (Friedman, Flynn-Wall-Ozawa (FWO) and Kissinger-Akahira-Sunose (KAS)) were compared with the Coats-Redfern method. The Ea values of devolatilization stages were in the range of 152-170kJ/mol, 148-178kJ/mol and 156-209kJ/mol for samples Pre, Dig R and SW, respectively. Concerning combustion process, char oxidation stages showed lower Ea values than that obtained for the combustion devolatilization stage, being in the range of 140-175kJ/mol, 178-199kJ/mol and 122-144kJ/mol for samples Pre, Dig R and SW, respectively. These results were practically the same for samples Pre and Dig R, which means that the kinetics of the thermochemical processes were not affected by anaerobic digestion. Finally, the distributed activation energy model (DAEM) and the pseudo-multi component stage model (PMSM) were applied to predict the weight loss curves of pyrolysis and combustion. DAEM was the best model that fitted the experimental data. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Application of Uncertainty and Sensitivity Analysis to a Kinetic Model for Enzymatic Biodiesel Production

    DEFF Research Database (Denmark)

    Price, Jason Anthony; Nordblad, Mathias; Woodley, John

    2014-01-01

    This paper demonstrates the added benefits of using uncertainty and sensitivity analysis in the kinetics of enzymatic biodiesel production. For this study, a kinetic model by Fedosov and co-workers is used. For the uncertainty analysis the Monte Carlo procedure was used to statistically quantify...

  8. Physico-Geometrical Kinetics of Solid-State Reactions in an Undergraduate Thermal Analysis Laboratory

    Science.gov (United States)

    Koga, Nobuyoshi; Goshi, Yuri; Yoshikawa, Masahiro; Tatsuoka, Tomoyuki

    2014-01-01

    An undergraduate kinetic experiment of the thermal decomposition of solids by microscopic observation and thermal analysis was developed by investigating a suitable reaction, applicable techniques of thermal analysis and microscopic observation, and a reliable kinetic calculation method. The thermal decomposition of sodium hydrogen carbonate is…

  9. Physico-Geometrical Kinetics of Solid-State Reactions in an Undergraduate Thermal Analysis Laboratory

    Science.gov (United States)

    Koga, Nobuyoshi; Goshi, Yuri; Yoshikawa, Masahiro; Tatsuoka, Tomoyuki

    2014-01-01

    An undergraduate kinetic experiment of the thermal decomposition of solids by microscopic observation and thermal analysis was developed by investigating a suitable reaction, applicable techniques of thermal analysis and microscopic observation, and a reliable kinetic calculation method. The thermal decomposition of sodium hydrogen carbonate is…

  10. [Culture medium optimization and primary kinetics analysis for nisin production].

    Science.gov (United States)

    Li, C; Bai, J H; Cai, Z L; Ouyang, F

    2001-03-01

    Response surface methodology was used to optimize a medium for nisin production of Lactococcus lactis. In the first optimization step the influence of sucrose, soybean peptone, yeast extract, potassium dihydrogen phosphate, sodium chloride, and magnesium sulfur on nisin production was evaluated using a fractional factorial design. Potassium dihydrogen phosphate influenced nisin production positively while soybean peptone affected nisin production negatively. The other components had no significant effect on nisin production. The path of steepest ascent was used to approach the optimal region of the medium composition. In the third step the optimal concentrations of KH2PO4 and soybean peptone were determined by a central composite design and response surface analysis. The optimized medium allowed nisin production to be increased from 1074 IU/mL to 2150 IU/mL. The kinetic analysis showed that nisin production fashion at optimized and non-optimized media was not changed and maintained partially growth-associated. But the specific growth rates and the specific nisin production rates for the strain at the optimized medium were bigger than the ones at the non-optimized medium after the cells entered the middle of exponential phase.

  11. Preliminary analysis of distributed in situ soil moisture measurements

    Directory of Open Access Journals (Sweden)

    L. Brocca

    2005-01-01

    Full Text Available Surface soil moisture content is highly variable in both space and time. Remote sensing can provide an effective methodology for mapping surface moisture content over large areas but ground based measurements are required to test its reliability and to calibrate retrieval algorithms. Recently, we had the opportunity to design and perform an experiment aimed at jointly acquiring measurements of surface soil water content at various locations and remotely sensed hyperspectral data. The area selected for the experiment is located in central Umbria and it extends for 90km2. For the area, detailed lithological and multi-temporal landslide inventory maps were available. We identified eight plots where measurements of soil water content were made using a Time Domain Reflectometer (TDR. The plots range in size from 100m2 to 600m2, and cover a variety of topographic and morphological settings. The TDR measurements were conducted during four days, on 5 April, 15 April, 2 May and 3 May 2004. On 3 May the NERC airborne CASI 2 acquired the hyperspectral data. Preliminary analysis concerning the matching between the landslides and the soil moisture were reported. Statistical and geostatistical analysis investigating the spatial-temporal soil moisture distribution were performed. These results will be compared with the data of surface temperature obtained from the remotely sensed hyperspectral sensor.

  12. Thermodynamic and kinetic analysis of heterogeneous photocatalysis for semiconductor systems.

    Science.gov (United States)

    Liu, Baoshun; Zhao, Xiujian; Terashima, Chiaki; Fujishima, Akira; Nakata, Kazuya

    2014-05-21

    Since the report of the Honda-Fujishima effect, heterogeneous photocatalysis has attracted much attention around the world because of its potential energy and environmental applications. Although great progresses have been made in recent years, most were focused on preparing highly-active photocatalysts and investigating visible light utilization. In fact, we are still unclear on the thermodynamic and kinetic nature of photocatalysis to date, which sometimes leads to misunderstandings for experimental results. It is timely to give a review and discussion on the thermodynamics and kinetics of photocatalysis, so as to direct future researches. However, there is an absence of a detailed review on this topic until now. In this article, we tried to review and discuss the thermodynamics and kinetics of photocatalysis. We explained the thermodynamic driving force of photocatalysis, and distinguished the functions of light and heat in photocatalysis. The Langmuir-Hinshelwood kinetic model, the ˙OH oxidation mechanism, and the direct-indirect (D-I) kinetic model were reviewed and compared. Some applications of the D-I model to study photocatalytic kinetics were also discussed. The electron transport mode and its importance in photocatalysis were investigated. Finally, the intrinsic relation between the kinetics and the thermodynamics of photocatalytic reactions was discussed.

  13. Thermogravimetric and Kinetic Analysis of Raw and Torrefied Biomass Combustion

    Directory of Open Access Journals (Sweden)

    Kopczyński Marcin

    2015-06-01

    Full Text Available The use of torrefied biomass as a substitute for untreated biomass may decrease some technological barriers that exist in biomass co-firing technologies e.g. low grindability, high moisture content, low energy density and hydrophilic nature of raw biomass. In this study the TG-MS-FTIR analysis and kinetic analysis of willow (Salix viminalis L. and samples torrefied at 200, 220, 240, 260, 280 and 300 °C (TSWE 200, 220, 240, 260, 280 and 300, were performed. The TG-DTG curves show that in the case of willow and torrefied samples TSWE 200, 220, 240 and 260 there are pyrolysis and combustion stages, while in the case of TSWE 280 and 300 samples the peak associated with the pyrolysis process is negligible, in contrast to the peak associated with the combustion process. Analysis of the TG-MS results shows m/z signals of 18, 28, 29 and 44, which probably represent H2O, CO and CO2. The gaseous products were generated in two distinct ranges of temperature. H2O, CO and CO2 were produced in the 500 K to 650 K range with maximum yields at approximately 600 K. In the second range of temperature, 650 K to 800 K, only CO2 was produced with maximum yields at approximately 710 K as a main product of combustion process. Analysis of the FTIR shows that the main gaseous products of the combustion process were H2O, CO2, CO and some organics including bonds: C=O (acids, aldehydes and ketones, C=C (alkenes, aromatics, C-O-C (ethers and C-OH. Lignin mainly contributes hydrocarbons (3000-2800 cm−1, while cellulose is the dominant origin of aldehydes (2860-2770 cm−1 and carboxylic acids (1790-1650 cm−1. Hydrocarbons, aldehydes, ketones and various acids were also generated from hemicellulose (1790-1650 cm−1. In the kinetic analysis, the two-steps first order model (F1F1 was assumed. Activation energy (Ea values for the first stage (pyrolysis increased with increasing torrefaction temperature from 93 to 133 kJ/mol, while for the second stage (combustion it

  14. Kinetic analysis of Legionella inactivation using ozone in wastewater.

    Science.gov (United States)

    Li, Jun; Li, Kunquan; Zhou, Yan; Li, Xuebin; Tao, Tao

    2017-02-01

    Legionella inactivation using ozone was studied in wastewater using kinetic analysis and modeling. The experimental results indicate that the relationship between the ozone concentration, germ concentration, and chemical oxygen demand (COD) can be used to predict variations in germ and COD concentrations. The ozone reaction with COD and inactivation of Legionella occurred simultaneously, but the reaction with COD likely occurred at a higher rate than the inactivation, as COD is more easily oxidized by ozone than Legionella. Higher initial COD concentrations resulted in a lower inactivation rate and higher lnN/N0. Higher temperature led to a higher inactivation efficiency. The relationship of the initial O3 concentration and Legionella inactivation rate was not linear, and thus, the Ct value required for a 99.99% reduction was not constant. The initial O3 concentration was more important than the contact time, and a reduction of the initial O3 concentration could not be compensated by increasing the contact time. The Ct values were compared over a narrow range of initial concentrations; the Ct values could only be contrasted when the initial O3 concentrations were very similar. A higher initial O3 concentration led to a higher inflection point value for the lnN/N0 vs C0t curve. Energy consumption using a plasma corona was lower than when using boron-doped diamond electrodes.

  15. Thermal decomposition kinetics of sorghum straw via thermogravimetric analysis.

    Science.gov (United States)

    Dhyani, Vaibhav; Kumar, Jitendra; Bhaskar, Thallada

    2017-09-01

    The thermal decomposition of sorghum straw was investigated by non-isothermal thermogravimetric analysis, where the determination of kinetic triplet (activation energy, pre-exponential factor, and reaction model), was the key objective. The activation energy was determined using different isoconversional methods: Friedman, Flynn-Wall-Ozawa (FWO), Kissinger-Akahira-Sunose (KAS), Starink, Iterative method of Chai & Chen, Vyazovkin AIC method, and Li & Tang equation. The pre-exponential factor was calculated using Kissinger's equation; while the reaction model was predicted by comparison of z-master plot obtained from experimental values with the theoretical plots. The values of activation energy obtained from isoconversional methods were further used for evaluation of thermodynamic parameters, enthalpy, entropy and Gibbs free energy. Results showed three zones of pyrolysis having average activation energy values of 151.21kJ/mol, 116.15kJ/mol, and 136.65kJ/mol respectively. The data was well fitting with two-dimension 'Valensi' model for conversion values from 0 to 0.4 with a coefficient of determination (R(2)) value of 0.988, and with third order reaction model for values from 0.4 to 0.9 with an R(2) value of 0.843. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Pyrolysis and combustion kinetics of lycopodium particles in thermogravimetric analysis

    Institute of Scientific and Technical Information of China (English)

    Seyed Alireza Mostafavi; Sadjad Salavati; Hossein Beidaghy Dizaji; Mehdi Bidabadi

    2015-01-01

    Biomass is a kind of renewable energy which is used increasingly in different types of combustion systems or in the production of fuels like bio-oil. Lycopodium is a cellulosic particle, with good combustion properties, of which microscopic images show that these particles have spherical shapes with identical diameters of 31 μm. The measured density of these particles is 1.0779 g/cm2. Lycopodium particles contain 64.06% carbon, 25.56% oxygen, 8.55% hydrogen and 1.83% nitrogen, and no sulfur. Thermogravimetric analysis in the nitrogen environment indicates that the maximum of particle mass reduction occurs in the temperature range of 250−550 °C where the maximum mass reduction in the DTG diagrams also occurs in. In the oxygen environment, an additional peak can also be observed in the temperature range of 500−600 °C, which points to solid phase combustion and ignition temperature of lycopodium particles. The kinetics of reactions is determined by curve fitting and minimization of error.

  17. Insulin dose response analysis of free fatty acid kinetics.

    Science.gov (United States)

    Jensen, Michael D; Nielsen, Søren

    2007-01-01

    Insulin regulation of free fatty acid (FFA) release is an important aspect of metabolic function; however, FFA release is exquisitely sensitive to insulin, which complicates the design and analysis of dose response experiments. We measured FFA ([(3)H]palmitate) and glucose ([(3)H]glucose) kinetics in 7 nonobese men, 7 nonobese women, 7 obese men, and 7 obese women by using a two-step insulin clamp (0.25 and 2.5 mU/kg fat-free mass per minute). Obese men and women were characterized as having a BMI of 28 or greater and body fat of 28% and 40% or greater for men and for women, respectively. Nonobese men and women had 22% and 35% or less body fat, respectively. All volunteers were Caucasian. Glucose disposal increased in a linear fashion with plasma insulin concentrations. The nonlinear suppression of plasma palmitate flux and concentrations could be linearized by logarithmically transforming both the insulin concentration and palmitate axes, except in nonobese men. We repeated the studies in 7 nonobese and 7 obese men, using 1.0 mU/kg fat-free mass per minute as the second insulin dose, which linearized the log-transformed lipolysis measures. The indices of insulin regulation of lipolysis predicted using 2 points (basal and second insulin dose) vs 3 points (basal, low, and high dose) were not different provided the proper second dose was selected. The EC(50) for insulin suppression of lipolysis correlated linearly with plasma triglycerides (r = 0.52, P < .001) and exponentially with insulin sensitivity(glucose) (r = 0.70, P < .001). We conclude that log transformation of insulin dose response data for FFA permits straightforward data analysis and simplifies the estimation of metabolically relevant parameters.

  18. Grid-connected ICES: preliminary feasibility analysis and evaluation. Volume 2. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1977-06-30

    The HEAL Complex in New Orleans will serve as a Demonstration Community for which the ICES Demonstration System will be designed. The complex is a group of hospitals, clinics, research facilities, and medical educational facilities. The five tasks reported on are: preliminary energy analysis; preliminary institutional assessment; conceptual design; firming-up of commitments; and detailed work management plan.

  19. Purification, crystallization and preliminary X-ray analysis of struthiocalcin 1 from ostrich (Struthio camelus) eggshell

    Energy Technology Data Exchange (ETDEWEB)

    Reyes-Grajeda, Juan Pablo [Unidad de Proteómica Médica, Instituto Nacional de Medicina Genómica, Mexico City (Mexico); Marín-García, Liliana [Instituto de Química, Universidad Nacional Autónoma de México (Mexico); Stojanoff, Vivian [Brookhaven National Laboratories, NSLS, Upton, New York (United States); Moreno, Abel, E-mail: carcamo@servidor.unam.mx [Instituto de Química, Universidad Nacional Autónoma de México (Mexico); Unidad de Proteómica Médica, Instituto Nacional de Medicina Genómica, Mexico City (Mexico)

    2007-11-01

    The purification, crystallization and preliminary X-ray diffraction data of the protein struthiocalcin 1 isolated from ostrich eggshell are reported. The purification, crystallization and preliminary X-ray analysis of struthiocalcin 1 (SCA-1), a protein obtained from the intramineral part of ostrich (Struthio camelus) eggshell, is reported.

  20. Investigation of Sorption and Diffusion Mechanisms, and Preliminary Economic Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Bhave, Ramesh R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Jubin, Robert Thomas [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Spencer, Barry B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Nair, Sankar [Georgia Inst. of Technology, Atlanta, GA (United States)

    2017-02-01

    This report describes the synthesis and evaluation of molecular sieve zeolite membranes to separate and concentrate tritiated water (HTO) from dilute HTO-bearing aqueous streams. Several monovalent and divalent cation exchanged silico alumino phosphate (SAPO-34) molecular sieve zeolite membranes were synthesized on disk supports and characterized with gas and vapor permeation measurements. The pervaporation process performance was evaluated for the separation and concentration of tritiated water. Experiments were performed using tritiated water feed solution containing tritium at the high end of the range (1 mCi/mL) anticipated in a nuclear fuel processing system that includes both acid and water streams recycling. The tritium concentration was about 0.1 ppm. The permeate was recovered under vacuum. The HTO/H2O selectivity and separation factor calculated from the measured tritium concentrations ranged from 0.99 to 1.23, and 0.83-0.98, respectively. Although the membrane performance for HTO separation was lower than expected, several encouraging observations including molecular sieving and high vapor permeance are reported. Additionally, several new approaches are proposed, such as tuning the sorption and diffusion properties offered by small pore LTA zeolite materials, and cation exchanged aluminosilicates with high metal loading. It is hypothesized that substantially improved preferential transport of tritium (HTO) resulting in a more concentrated permeate can be achieved. Preliminary economic analysis for the membrane-based process to concentrate tritiated water is also discussed.

  1. Crystallization and preliminary crystallographic analysis of recombinant human galectin-1

    Energy Technology Data Exchange (ETDEWEB)

    Scott, Stacy A. [Institute for Glycomics, Gold Coast Campus, Griffith University, Queensland 4222 (Australia); Scott, Ken [School of Biological Sciences, University of Auckland, Auckland (New Zealand); Blanchard, Helen, E-mail: h.blanchard@griffith.edu.au [Institute for Glycomics, Gold Coast Campus, Griffith University, Queensland 4222 (Australia)

    2007-11-01

    Human galectin-1 has been cloned, expressed in E. coli, purified and crystallized in the presence of both lactose (ligand) and β-mercaptoethanol under six different conditions. The X-ray diffraction data obtained have enabled the assignment of unit-cell parameters for two novel crystal forms of human galectin-1. Galectin-1 is considered to be a regulator protein as it is ubiquitously expressed throughout the adult body and is responsible for a broad range of cellular regulatory functions. Interest in galectin-1 from a drug-design perspective is founded on evidence of its overexpression by many cancers and its immunomodulatory properties. The development of galectin-1-specific inhibitors is a rational approach to the fight against cancer because although galectin-1 induces a plethora of effects, null mice appear normal. X-ray crystallographic structure determination will aid the structure-based design of galectin-1 inhibitors. Here, the crystallization and preliminary diffraction analysis of human galectin-1 crystals generated under six different conditions is reported. X-ray diffraction data enabled the assignment of unit-cell parameters for crystals grown under two conditions, one belongs to a tetragonal crystal system and the other was determined as monoclinic P2{sub 1}, representing two new crystal forms of human galectin-1.

  2. Preliminary radiation criteria and nuclear analysis for ETF

    Energy Technology Data Exchange (ETDEWEB)

    Engholm, B.A.

    1980-09-01

    Preliminary biological and materials radiation dose criteria for the Engineering Test Facility are described and tabulated. In keeping with the ETF Mission Statement, a key biological dose criterion is a 24-hour shutdown dose rate of 2 mrem/hr on the surface of the outboard bulk shield. Materials dose criteria, which primarily govern the inboard shield design, include 10/sup 9/ rads exposure limit to epoxy insulation, 3 x 10/sup -4/ dpa damage to the TF coil copper stabilizer, and a total nuclear heating rate of 5 kW in the inboard TF coils. Nuclear analysis performed during FY 80 was directed primarily at the inboard and outboard bulk shielding, and at radiation streaming in the neutral beam drift ducts. Inboard and outboard shield thicknesses to achieve the biological and materials radiation criteria are 75 cm inboard and 125 cm outboard, the configuration consisting of alternating layers of stainless steel and borated water. The outboard shield also includes a 5 cm layer of lead. NBI duct streaming analyses performed by ORNL and LASL will play a key role in the design of the duct and NBI shielding in FY 81. The NBI aluminum cryopanel nuclear heating rate during the heating cycle is about 1 milliwatt/cm/sup 3/, which is far less than the permissible limit.

  3. Preliminary analysis of aerial hyperspectral data on shallow lacustrine waters

    Science.gov (United States)

    Bianchi, Remo; Castagnoli, A.; Cavalli, Rosa M.; Marino, Carlo M.; Pignatti, Stefano; Zilioli, Eugenio

    1995-11-01

    The availability of MIVIS hyperspectral data, deriving from an aerial survey recently performed over a test-site in Lake Garda, Italy, gave the possibility of a preliminary new insight in the field of specific applications of remote sensing to shallow water analysis. The spectroradiometers in the visible and in the thermal infrared were explored in particular, accessing to helpful information for the detection of bio-physical indicators of water quality, either related to the surface/sub-surface of waters or to the bottom of the lake, since the study area presents very shallow waters, never exceeding a 6-meter depth in any case. Primary interest was the detection of man-induced activities along the margins, like sewage effect and sedimentary structure in the bottom or algal bloom. Secondly, a correlation between absorbivity coefficients in the visible bands and bathimetric contour lines in the proximity of the marginal zone of the lake was accomplished, by means of two indicative spectroradiometric transects.

  4. Preliminary safety analysis for key design features of KALIMER

    Energy Technology Data Exchange (ETDEWEB)

    Hahn, D. H.; Kwon, Y. M.; Chang, W. P.; Suk, S. D.; Lee, S. O.; Lee, Y. B.; Jeong, K. S

    2000-07-01

    KAERI is currently developing the conceptual design of a liquid metal reactor, KALIMER(Korea Advanced Liquid Metal Reactor) under the long-term nuclear R and D program. In this report, descriptions of the KALIMER safety design features and safety analyses results for selected ATWS accidents are presented. First, the basic approach to achieve the safety goal is introduced in chapter 1, and the safety evaluation procedure for the KALIMER design is described in chapter 2. It includes event selection, event categorization, description of design basis events, and beyond design basis events. In chapter 3, results of inherent safety evaluations for the KALIMER conceptual design are presented. The KALIMER core and plant system are designed to assure design performance during a selected set of events without either reactor control or protection system intervention. Safety analyses for the postulated anticipated transient without scram(ATWS) have been performed to investigate the KALIMER system response to the events. They are categorized as bounding events(BEs) because of their low probability of occurrence. In chapter 4, the design of the KALIMER containment dome and the results of its performance analysis are presented. The designs of the existing LMR containment and the KALIMER containment dome have been compared in this chapter. Procedure of the containment performance analysis and the analysis results are described along with the accident scenario and source terms. Finally, a simple methodology is introduced to investigate the core kinetics and hydraulic behavior during HCDA in chapter 5. Mathematical formulations have been developed in the framework of the modified bethe-tait method, and scoping analyses have been performed for the KALIMER core behavior during super-prompt critical excursions.

  5. Preliminary Analysis of Two Years of the Massive Collision Monitoring Activity

    Science.gov (United States)

    McKnight, Darren; Matney, Mark; Walbert, Kris; Behrend, Sophie; Casey, Patrick; Speaks, Seth

    2017-01-01

    It is hypothesized that the interactions between many of the most massive derelicts in low Earth orbit are more frequent than modeled by the traditional combination of kinetic theory of gases and Poisson probability distribution function. This is suggested by the fact that there are clusters of derelicts where members' inclinations are nearly identical and their apogees/perigees overlap significantly resulting in periodic synchronization of the objects' orbits. In order to address this proposition, an experiment was designed and conducted over the last two years. Results from this monitoring and characterization experiment are presented with implications for proposed debris remediation strategies. Four separate clusters of massive derelicts were examined that are centered around 775km, 850km, 975km, and 1500km, respectively. In aggregate, the constituents of these clusters contain around 500 objects and about 800,000kg of mass; this equates to a third of all derelict mass in LEO. Preliminary analysis indicates that encounter rates over this time period for these objects are greater than is estimated by traditional techniques. Hypothesized dependencies between latitude of encounter, relative velocity, frequency of encounters, inclination, and differential semi-major axis were established and verified. This experiment also identified specific repeatable cluster dynamics that may reduce the cost/risk and enhance the effectiveness of debris remediation activities and also enable new operational debris remediation options.

  6. Evaluation of reliability of Coats-Redfern method for kinetic analysis of non-isothermal TGA

    Institute of Scientific and Technical Information of China (English)

    R. Ebrahimi-Kahrizsangi; M. H. Abbasi

    2008-01-01

    A critical examination was made on the reliability of kinetic parameters of nonisothermal thermoanalytical rate measurement by the widely applied Coats-Redfern(CR) equation. For this purpose, simulated TGA curves were made for reactions with different kinetic models, including chemical, diffusion (Janders) and mixed mechanism at different heating rates. The results show that, for reactions controlled kinetically by one mechanism, all solid state reaction models show linear trends by use of CR method and this method can not distinct the correct reaction model. For reactions with mixed mechanism, the CR method shows nonlinear trends and the reaction models and kinetic parameters can not be extracted from CR curves. The overall conclusion from this comparative appraisal of the characteristics of the CR approach to kinetic analysis of TGA data is that the CR approach is generally unsuitable for determination of kinetic parameters.

  7. Conversion Preliminary Safety Analysis Report for the NIST Research Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Diamond, D. J. [Brookhaven National Lab. (BNL), Upton, NY (United States); Baek, J. S. [Brookhaven National Lab. (BNL), Upton, NY (United States); Hanson, A. L. [Brookhaven National Lab. (BNL), Upton, NY (United States); Cheng, L-Y [Brookhaven National Lab. (BNL), Upton, NY (United States); Brown, N. [Brookhaven National Lab. (BNL), Upton, NY (United States); Cuadra, A. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2015-01-30

    The NIST Center for Neutron Research (NCNR) is a reactor-laboratory complex providing the National Institute of Standards and Technology (NIST) and the nation with a world-class facility for the performance of neutron-based research. The heart of this facility is the NIST research reactor (aka NBSR); a heavy water moderated and cooled reactor operating at 20 MW. It is fueled with high-enriched uranium (HEU) fuel elements. A Global Threat Reduction Initiative (GTRI) program is underway to convert the reactor to low-enriched uranium (LEU) fuel. This program includes the qualification of the proposed fuel, uranium and molybdenum alloy foil clad in an aluminum alloy, and the development of the fabrication techniques. This report is a preliminary version of the Safety Analysis Report (SAR) that would be submitted to the U.S. Nuclear Regulatory Commission (NRC) for approval prior to conversion. The report follows the recommended format and content from the NRC codified in NUREG-1537, “Guidelines for Preparing and Reviewing Applications for the Licensing of Non-power Reactors,” Chapter 18, “Highly Enriched to Low-Enriched Uranium Conversions.” The emphasis in any conversion SAR is to explain the differences between the LEU and HEU cores and to show the acceptability of the new design; there is no need to repeat information regarding the current reactor that will not change upon conversion. Hence, as seen in the report, the bulk of the SAR is devoted to Chapter 4, Reactor Description, and Chapter 13, Safety Analysis.

  8. Preliminary Analysis of Remote Monitoring & Robotic Concepts for Performance Confirmation

    Energy Technology Data Exchange (ETDEWEB)

    D.A. McAffee

    1997-02-18

    ) Identify and discuss the main Performance Confirmation monitoring needs and requirements during the post-emplacement preclosure period. This includes radiological, non-radiological, host rock, and infrastructure performance monitoring needs. It also includes monitoring for possible off-normal events. (Presented in Section 7.3). (3) Identify general approaches and methods for obtaining performance information from within the emplacement drifts for Performance Confirmation. (Presented in Section 7.4) (4)Review and discuss available technologies and design strategies that may permit the use of remotely operated systems within the hostile thermal and radiation environment expected within the emplacement drifts. (Presented in Section 7.5). (5) Based on Performance Confirmation monitoring needs and available technologies, identify potential application areas for remote systems and robotics for post-emplacement preclosure Performance Confirmation activities (Presented in Section 7.6). (6) Develop preliminary remote monitoring and robotic concepts for post-emplacement, preclosure Performance Confirmation activities. (Presented in Section 7.7) This analysis is being performed very early in the systems engineering cycle, even as issues related to the Performance Confirmation program planning phase are being formulated and while the associated needs, constraints and objectives are yet to be fully determined and defined. This analysis is part of an issue formulation effort and is primarily concerned with identification and description of key issues related to remotely monitoring repository performance for Performance Confirmation. One of the purposes of this analysis is to provide an early investigation of potential design challenges that may have a high impact on future design concepts. This analysis can be used to guide future concept development and help access what is feasible and achievable by application of remote systems technology. Future design and systems engineering

  9. TChem - A Software Toolkit for the Analysis of Complex Kinetic Models

    Energy Technology Data Exchange (ETDEWEB)

    Safta, Cosmin [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Najm, Habib N. [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Knio, Omar [Johns Hopkins Univ., Baltimore, MD (United States)

    2011-05-01

    The TChem toolkit is a software library that enables numerical simulations using complex chemistry and facilitates the analysis of detailed kinetic models. The toolkit provide capabilities for thermodynamic properties based on NASA polynomials and species production/consumption rates. It incorporates methods that can selectively modify reaction parameters for sensitivity analysis. The library contains several functions that provide analytically computed Jacobian matrices necessary for the efficient time advancement and analysis of detailed kinetic models.

  10. A Preliminary Tsunami vulnerability analysis for Bakirkoy district in Istanbul

    Science.gov (United States)

    Tufekci, Duygu; Lutfi Suzen, M.; Cevdet Yalciner, Ahmet; Zaytsev, Andrey

    2016-04-01

    Resilience of coastal utilities after earthquakes and tsunamis has major importance for efficient and proper rescue and recovery operations soon after the disasters. Vulnerability assessment of coastal areas under extreme events has major importance for preparedness and development of mitigation strategies. The Sea of Marmara has experienced numerous earthquakes as well as associated tsunamis. There are variety of coastal facilities such as ports, small craft harbors, and terminals for maritime transportation, water front roads and business centers mainly at North Coast of Marmara Sea in megacity Istanbul. A detailed vulnerability analysis for Yenikapi region and a detailed resilience analysis for Haydarpasa port in Istanbul have been studied in previously by Cankaya et al., (2015) and Aytore et al., (2015) in SATREPS project. In this study, the methodology of vulnerability analysis under tsunami attack given in Cankaya et al., (2015) is modified and applied to Bakirkoy district of Istanbul. Bakirkoy district is located at western part of Istanbul and faces to the North Coast of Marmara Sea from 28.77oE to 28.89oE. High resolution spatial dataset of Istanbul Metropolitan Municipality (IMM) is used and analyzed. The bathymetry and topography database and the spatial dataset containing all buildings/structures/infrastructures in the district are collated and utilized for tsunami numerical modeling and following vulnerability analysis. The tsunami parameters from deterministically defined worst case scenarios are computed from the simulations using tsunami numerical model NAMI DANCE. The vulnerability assessment parameters in the district according to vulnerability and resilience are defined; and scored by implementation of a GIS based TVA with appropriate MCDA methods. The risk level is computed using tsunami intensity (level of flow depth from simulations) and TVA results at every location in Bakirkoy district. The preliminary results are presented and discussed

  11. Preliminary scaling laws for plasma current, ion kinetic temperature, and plasma number density in the NASA Lewis bumpy torus plasma

    Science.gov (United States)

    Roth, J. R.

    1976-01-01

    Parametric variation of independent variables which may affect the characteristics of bumpy torus plasma have identified those which have a significant effect on the plasma current, ion kinetic temperature, and plasma number density, and those which do not. Empirical power law correlations of the plasma current, and the ion kinetic temperature and number density were obtained as functions of potential applied to the midplane electrode rings, the background neutral gas pressure, and the magnetic field strength. Additional parameters studied included the type of gas, the polarity of the midplane electrode rings, the mode of plasma operation, and the method of measuring the plasma number density. No significant departures from the scaling laws appear to occur at the highest ion kinetic temperatures or number densities obtained to date.

  12. Analysis, evaluation, and optimization of kinetic parameters for performance appraisal and design of UASB reactors.

    Science.gov (United States)

    Bhunia, Puspendu; Ghangrekar, M M

    2008-05-01

    Studies have been undertaken to explore the applicability of different kinetic models for the performance appraisal of upflow anaerobic sludge blanket (UASB) reactors treating wastewater in the range of 300-4000 mg COD/l. Three kinetic models namely, Monod, Grau second-order, and Haldane model are considered for the analysis. Both linear and nonlinear regressions have been performed to examine the best-fit among the kinetic models. In this process, five error analysis methods have been used to analyze the data. Apart from optimization of kinetic coefficients with minimization of associated errors, prediction of effluent COD has also been undertaken to verify the applicability of kinetic models. In both the cases, Grau second-order model is found to be the best class of fit for wide range of data sets in UASB reactor.

  13. Preliminary Core Analysis of a Micro Modular Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Jo, Chang Keun; Chang, Jongwa [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Venneri, Francesco [Ultra Safe Nuclear Corporation, Los Alamos (United States); Hawari, Ayman [NC State Univ., Raleigh (United States)

    2014-05-15

    The Micro Modular Reactor (MMR) will be 'melt-down proof'(MDP) under all circumstances, including the complete loss of coolant, and will be easily transportable and retrievable, and suitable for use with very little site preparation and Balance of Plant (BOP) requirements for a variety of applications, from power generation and process heat applications in remote areas to grid-unattached locations, including ship propulsion. The Micro Modular Reactor design proposed in this paper has 3 meter diameter core (2 meter active core) which is suitable for 'factory manufactured' and has few tens year of service life for remote deployment. We confirmed the feasibility of long term service life by a preliminary neutronic analysis in terms of the excess reactivity, the temperature feedback coefficient, and the control margins. We are able to achieve a reasonably long core life time of 5 ∼ 10 years under typical thermal hydraulic condition of a helium cooled reactor. However, on a situation where longer service period and safety is important, we can reduce the power density to the level of typical pebble bed reactor. In this case we can design 10 MWt MMR with core diameter for 10 ∼ 40 years core life time without much loss in the economics. Several burnable poisons are studied and it is found that erbia mixed in the compact matrix seems reasonably good poison. The temperature feedback coefficients were remaining negative during lifetime. Drum type control rods at reflector region and few control rods inside core region are sufficient to control the reactivity during operation and to achieve safe cold shutdown state.

  14. NMR analysis of base-pair opening kinetics in DNA

    Science.gov (United States)

    Szulik, Marta W.; Voehler, Markus; Stone, Michael P.

    2014-01-01

    Base pairing in nucleic acids plays a crucial role in their structure and function. Differences in the base pair opening and closing kinetics of individual double stranded DNA sequences or between chemically modified base pairs provide insight into the recognition of these base pairs by DNA processing enzymes. This unit describes how to quantify the kinetics for localized base pairs by observing changes in the imino proton signals by nuclear magnetic resonance spectroscopy. The determination of all relevant parameters using state of the art techniques and NMR instrumentation, including cryoprobes, is discussed. PMID:25501592

  15. Preliminary evaluation of diabatic heating distribution from FGGE level 3b analysis data

    Science.gov (United States)

    Kasahara, A.; Mizzi, A. P.

    1985-01-01

    A method is presented for calculating the global distribution of diabatic heating rate. Preliminary results of global heating rate evaluated from the European center for Medium Range Weather Forecasts Level IIIb analysis data is also presented.

  16. The Organic Food Market and Marketing Initiatives in Europe: a Preliminary Analysis

    DEFF Research Database (Denmark)

    Kristensen, Niels Heine; Nielsen, Thorkild; Bruselius-Jensen, Maria Louisa

    2003-01-01

    Kristensen NH, Nielsen T, Bruselius-Jensen M, Scheperlen-Bøgh P, Beckie M, Foster C, Midmore P, Padel S (2002): The Organic Food Market and Marketing Initiatives in Europe: a Preliminary Analysis. Final Report to the EU Commission......Kristensen NH, Nielsen T, Bruselius-Jensen M, Scheperlen-Bøgh P, Beckie M, Foster C, Midmore P, Padel S (2002): The Organic Food Market and Marketing Initiatives in Europe: a Preliminary Analysis. Final Report to the EU Commission...

  17. Kinetic analysis of nitric oxide reduction using biogas as reburning fuel

    African Journals Online (AJOL)

    STORAGESEVER

    2009-05-18

    May 18, 2009 ... reburnning technology potential to reduce NO pollution. Key word: Biomass, biogas, kinetic analysis, modeling, nitric oxide, reburning. INTRODUCTION .... factor A, the temperature β, and the activation energy E are specified.

  18. Growth of Listeria monocytogenes in Salmon Roe - a kinetic analysis

    Science.gov (United States)

    The objective of this study was to investigate the growth kinetics of Listeria monocytogenes in unsalted and salted (3%) salmon roe. Growth curves, developed using inoculated samples incubated at constant temperatures between 5 and 30 degrees C, were analyzed by curve-fitting to the Huang and Baran...

  19. Steady-State Kinetic Analysis of DNA Polymerase Single-Nucleotide Incorporation Products

    Science.gov (United States)

    O'Flaherty, Derek K.

    2014-01-01

    This unit describes the experimental procedures for the steady-state kinetic analysis of DNA synthesis across DNA nucleotides (native or modified) by DNA polymerases. In vitro primer extension experiments with a single nucleoside triphosphate species followed by denaturing polyacrylamide gel electrophoresis of the extended products is described. Data analysis procedures and fitting to steady-state kinetic models is presented to highlight the kinetic differences involved in the bypass of damaged versus undamaged DNA. Moreover, explanations concerning problems encountered in these experiments are addressed. This approach provides useful quantitative parameters for the processing of damaged DNA by DNA polymerases. PMID:25501593

  20. Preliminary study on washability and composition analysis of highsulfur coal in some mining areas in Guizhou

    Institute of Scientific and Technical Information of China (English)

    QIU Yue-qin; MAO Song; ZHANG Qin; TIAN Ye; LIU Zhi-hong

    2011-01-01

    Preliminary sink-float experiments on high-sulfur coal was done in some mining areas and carried on elementary analysis, industrial analysis, and ashcontent analysis. Through the experiments, definite middlings, and gangue, the phase analysis of sulfur was carried on, by which a good understanding of sulfur characters in raw coal was achieved.

  1. Uptake and tracer kinetics of O-(2-{sup 18}F-fluoroethyl)-l-tyrosine in meningiomas: preliminary results

    Energy Technology Data Exchange (ETDEWEB)

    Cornelius, Jan F.; Slotty, Philipp; Kamp, Marcel; El Khatib, Mustafa; Haenggi, Daniel; Sabel, Michael; Steiger, Hans Jakob [Heinrich Heine University, Department of Neurosurgery, Duesseldorf (Germany); Stoffels, Gabriele; Filss, Christian; Shah, Nadim J. [Institute of Neuroscience and Medicine, Forschungszentrum Juelich, Juelich (Germany); Galldiks, Norbert [Institute of Neuroscience and Medicine, Forschungszentrum Juelich, Juelich (Germany); University of Cologne, Department of Neurology, Cologne (Germany); Felsberg, Joerg [University of Duesseldorf, Department of Neuropathology, Duesseldorf (Germany); Coenen, Heinz H. [Institute of Neuroscience and Medicine, Forschungszentrum Juelich, Juelich (Germany); Juelich-Aachen Research Alliance (JARA) - Section JARA-Brain, Juelich (Germany); Langen, Karl-Josef [Institute of Neuroscience and Medicine, Forschungszentrum Juelich, Juelich (Germany); University of Aachen, Department of Nuclear Medicine, Aachen (Germany)

    2014-10-21

    O-(2-[{sup 18}F]Fluoroethyl)-l-tyrosine ({sup 18}F-FET) is a well-established PET tracer for the imaging of cerebral gliomas, but little is known about {sup 18}F-FET uptake in meningiomas. The aim of this study was to explore {sup 18}F-FET kinetics and tumour-to-background contrast in meningiomas of various histologies. A group of 24 patients with suspected cerebral meningioma on MRI/CT had an additional dynamic {sup 18}F-FET PET scan prior to surgery. Time-activity curves (TAC) of {sup 18}F-FET uptake in the tumours and tumour-to-brain ratios (TBR) for early (3 - 14 min after injection) and late {sup 18}F-FET uptake (20 - 40 min after injection) were analysed and compared with histological subtypes and WHO grade. {sup 18}F-FET uptake in critical structures in the skull base was also evaluated in terms of tumour-to-tissue (T/Tis) ratio. TBR of {sup 18}F-FET uptake in meningiomas was significantly higher in the early phase than in the late phase (3.5 ± 0.8 vs. 2.2 ± 0.3; P < 0.001). The difference in TBR between low-grade meningiomas (WHO grade I, 18 patients) and high-grade meningiomas (WHO grade II or III, 6 patients) was significant in the late phase of {sup 18}F-FET uptake (2.1 ± 0.2 vs. 2.5 ± 0.2, P = 0.003) while there was no significant difference in the early phase. ROC analysis showed that TBR of {sup 18}F-FET uptake in the late phase had significant power to differentiate low-grade from high-grade meningiomas (AUC 0.87 ± 0.18, sensitivity 83 %, specificity 83 %, optimal cut-off 2.3; P < 0.01). Evaluation of TAC yielded three different curve patterns of {sup 18}F-FET PET uptake. Combination of TBR (cut-off value 2.3) and TAC pattern slightly improved the differentiation of high-grade from low-grade meningiomas (accuracy 92 %; P = 0.001). Analysis of background radioactivity in the skull base indicated that {sup 18}F-FET uptake may be helpful in distinguishing meningioma tissue in the late phase. T/Tis ratios were >1.2 in all patients for the periorbita

  2. Cusum charts for preliminary analysis of individual observations

    NARCIS (Netherlands)

    A.J. Koning (Alex); R.J.M.M. Does (Ronald)

    1997-01-01

    textabstractA preliminary Cusum chart based on individual observations is developed from the uniformly most powerful test for the detection of linear trends. This Cusum chart is compared with several of its competitors which are based on the likelihood ratio test and on transformations of standardiz

  3. Co-combustion of different sewage sludge and coal: a non-isothermal thermogravimetric kinetic analysis.

    Science.gov (United States)

    Otero, M; Calvo, L F; Gil, M V; García, A I; Morán, A

    2008-09-01

    The kinetics of the combustion of coal, two different sewage sludge and their blends (containing different dried weight percentages of sewage sludge) was studied by simultaneous thermogravimetric analysis. Once the weight percentage of sludge in the blend was 10%, the effects on the combustion of coal were hardly noticeable in terms of weight loss. The Arrhenius activation energy corresponding to the co-combustion of the blends was evaluated by non-isothermal kinetic analysis. This showed that, though differences between coal and sewage sludge, the combustion of their blends kept kinetically alike to that of the coal. This work illustrates how thermogravimetric analysis may be used as an easy rapid tool to asses, not only mass loss, but also kinetics of the co-combustion of sewage sludge and coal blends.

  4. Analysis of TL and OSL kinetics of lithium aluminate

    CERN Document Server

    Twardak, A; Marczewska, B; Gieszczyk, W

    2014-01-01

    Lithium aluminate (LiAlO2) polycrystalline material showing high OSL sensitivity and linear dose response was prepared at IFJ Krakow. The kinetic parameters of OSL and TL processes were evaluated using various experimental techniques: LM-OSL deconvolution, TL glow-curve deconvolution, variable heating rate and isothermal decay. The OSL signal was found to consist of four components, one of them exhibiting a very slow decay. The TL glow-curve possesses two apparent peaks at approximately 85 oC and at 165 oC, which both seem to follow first order kinetics. These peaks seem to have a composite structure and as many as six peaks were found in the glow-curve.

  5. Systems engineering analysis of kinetic energy weapon concepts

    Energy Technology Data Exchange (ETDEWEB)

    Senglaub, M.

    1996-06-01

    This study examines, from a systems engineering design perspective, the potential of kinetic energy weapons being used in the role of a conventional strategic weapon. Within the Department of Energy (DOE) complex, strategic weapon experience falls predominantly in the nuclear weapons arena. The techniques developed over the years may not be the most suitable methodologies for use in a new design/development arena. For this reason a more fundamental approach was pursued with the objective of developing an information base from which design decisions might be made concerning the conventional strategic weapon system concepts. The study examined (1) a number of generic missions, (2) the effects of a number of damage mechanisms from a physics perspective, (3) measures of effectiveness (MOE`s), and (4) a design envelope for kinetic energy weapon concepts. With the base of information a cut at developing a set of high-level system requirements was made, and a number of concepts were assessed against these requirements.

  6. Systems engineering analysis of kinetic energy weapon concepts

    Energy Technology Data Exchange (ETDEWEB)

    Senglaub, M.

    1996-06-01

    This study examines, from a systems engineering design perspective, the potential of kinetic energy weapons being used in the role of a conventional strategic weapon. Within the Department of Energy (DOE) complex, strategic weapon experience falls predominantly in the nuclear weapons arena. The techniques developed over the years may not be the most suitable methodologies for use in a new design/development arena. For this reason a more fundamental approach was pursued with the objective of developing an information base from which design decisions might be made concerning the conventional strategic weapon system concepts. The study examined (1) a number of generic missions, (2) the effects of a number of damage mechanisms from a physics perspective, (3) measures of effectiveness (MOE`s), and (4) a design envelope for kinetic energy weapon concepts. With the base of information a cut at developing a set of high-level system requirements was made, and a number of concepts were assessed against these requirements.

  7. Thermal analysis and combustion kinetic of heavy oils

    Energy Technology Data Exchange (ETDEWEB)

    Santos, R.G. [Centre for Petroleum Studies, State University of Campinas(Brazil); Vargas, J.A.V.; Trevisan, O.V. [Department of Petroleum Engineering, Faculty of Mechanical Engineering, State University of Campinas (Brazil)

    2011-07-01

    In the oilfield sector, a thermal method named in-situ combustion (ISC) is used as an enhanced recovery method. ISC consists of the injection of gas into the reservoir, a combustion front is created producing heat which reduces the oil viscosity. For this method to be successful, understanding of the thermal and kinetic parameters involved is required; the aim of this paper is to evaluate those parameters for different crude oils. Experiments were conducted using accelerating rate calorimetry on Brazilian heavy oil samples under a heat-wait-seek-mode. Results showed that accelerating rate calorimetry is efficient in resolving the three main regions of reaction of the oil and that between 200 degree C and 300 degree C oxygen addition reactions are dominant while bond scission reactions dominate from 350 degree C. This study demonstrated that accelerating rate calorimetry is an efficient method to determine thermal and kinetic parameters of oxidation reaction of heavy oil.

  8. Kinetic analysis of supine stepping for early rehabilitation of walking.

    Science.gov (United States)

    Fang, Juan; Galen, Sujay; Vuckovic, Aleksandra; Conway, Bernard A; Hunt, Kenneth J

    2014-05-01

    In order to promote gait restoration in patients who cannot maintain an upright position in the early post-injury phase, a Gait Orthosis for Early Rehabilitation was proposed for supine stepping. Although supine stepping can generate lower-limb joint trajectories which are close to normal gait, the inter-segmental dynamics of supine stepping are believed to be different from those of upright walking. Furthermore, training in a supine position requires a certain loading on the foot to mimic the ground reaction forces, where different loading amplitudes influence the joint dynamics. This work analysed the kinetics of supine stepping with variable loading and investigated structural modifications for the Gait Orthosis for Early Rehabilitation system to address this kinetic difference. Three able-bodied subjects walked overground while their walking performance was recorded. Based on the experimental data, a leg-linkage model was developed to simulate the dynamics of upright walking. This model was then rotated by 90° with different foot loadings to investigate the kinetics of supine stepping. Compared to upright walking, supine stepping had a large kinetic difference at the hip joint due to the supine leg position. The ankle joint during supine stepping was sensitive to the force amplitude simulated on the foot. Thus, the Gait Orthosis for Early Rehabilitation system requires a leg frame to compensate the position change and a shoe platform to activate the leg muscles, especially at the ankle joint. This study provided important structural information for the further development of the Gait Orthosis for Early Rehabilitation system.

  9. Current Mooring Design in Partner WECs and Candidates for Preliminary Analysis

    DEFF Research Database (Denmark)

    Thomsen, Jonas Bjerg; Ferri, Francesco; Kofoed, Jens Peter

    This report is the combined report of Commercial Milestone "CM1: Design and Cost of Current Mooring Solutions of Partner WECs" and Milestone "M3: Mooring Solutions for Preliminary Analysis" of the EUDP project "Mooring Solutions for Large Wave Energy Converters". The report covers a description...... of the current mooring design of the partner Wave Energy Converter (WEC) developers in the project, together with a preliminary cost estimate of the systems....

  10. Preliminary Dynamic Siol-Structure-Interaction Analysis for the Waste Handling Building

    Energy Technology Data Exchange (ETDEWEB)

    G. Wagenblast

    2000-05-01

    The objective of this analysis package is to document a preliminary dynamic seismic evaluation of a simplified design concept of the Wade Handling Building (WHB). Preliminary seismic ground motions and soil data will be used. Loading criteria of the WHB System Design Description will be used. Detail design of structural members will not be performed.. The results of the analysis will be used to determine preliminary sizes of structural concrete and steel members and to determine whether the seismic response of the structure is within an acceptable level for future License Application design of safety related facilities. In order to complete this preliminary dynamic evaluation to meet the Site Recommendation (SR) schedule, the building configuration was ''frozen in time'' as the conceptual design existed in October 1999. Modular design features and dry or wet waste storage features were intentionally excluded from this preliminary dynamic seismic evaluation. The document was prepared in accordance with the Development Plan for the ''Preliminary/Dynamic Soil Structure Interaction Analysis for the Waste Handling Building'' (CRWMS M&O 2000b), which was completed, in accordance with AP-2.13Q, ''Technical Product Development Planning''.

  11. Pre-Steady-State Kinetic Analysis of Single-Nucleotide Incorporation by DNA Polymerases.

    Science.gov (United States)

    Su, Yan; Peter Guengerich, F

    2016-06-01

    Pre-steady-state kinetic analysis is a powerful and widely used method to obtain multiple kinetic parameters. This protocol provides a step-by-step procedure for pre-steady-state kinetic analysis of single-nucleotide incorporation by a DNA polymerase. It describes the experimental details of DNA substrate annealing, reaction mixture preparation, handling of the RQF-3 rapid quench-flow instrument, denaturing polyacrylamide DNA gel preparation, electrophoresis, quantitation, and data analysis. The core and unique part of this protocol is the rationale for preparation of the reaction mixture (the ratio of the polymerase to the DNA substrate) and methods for conducting pre-steady-state assays on an RQF-3 rapid quench-flow instrument, as well as data interpretation after analysis. In addition, the methods for the DNA substrate annealing and DNA polyacrylamide gel preparation, electrophoresis, quantitation and analysis are suitable for use in other studies. © 2016 by John Wiley & Sons, Inc.

  12. Evaluation of kinetic phosphorescence analysis for the determination of uranium

    Energy Technology Data Exchange (ETDEWEB)

    Croatto, P.V.; Frank, I.W.; Johnson, K.D.; Mason, P.B.; Smith, M.M.

    1997-12-01

    In the past, New Brunswick Laboratory (NBL) has used a fluorometric method for the determination of sub-microgram quantities of uranium. In its continuing effort to upgrade and improve measurement technology, NBL has evaluated the commercially-available KPA-11 kinetic phosphorescence analyzer (Chemchek, Richland, WA). The Chemchek KPA-11 is a bench-top instrument which performs single-measurement, quench-corrected analyses for trace uranium. It incorporates patented kinetic phosphorimetry techniques to measure and analyze sample phosphorescence as a function of time. With laser excitation and time-corrected photon counting, the KPA-11 has a lower detection limit than conventional fluorometric methods. Operated with a personal computer, the state-of-the-art KPA-11 offers extensive time resolution and phosphorescence lifetime capabilities for additional specificity. Interferences are thereby avoided while obtaining precise measurements. Routine analyses can be easily and effectively accomplished, with the accuracy and precision equivalent to the pulsed-laser fluorometric method presently performed at NBL, without the need for internal standards. Applications of kinetic phosphorimetry at NBL include the measurement of trace level uranium in retention tank, waste samples, and low-level samples. It has also been used to support other experimental activities at NBL by the measuring of nanogram amounts of uranium contamination (in blanks) in isotopic sample preparations, and the determining of elution curves of different ion exchange resins used for uranium purification. In many cases, no pretreatment of samples was necessary except to fume them with nitric acid, and then to redissolve and dilute them to an appropriate concentration with 1 M HNO{sub 3} before measurement. Concentrations were determined on a mass basis ({micro}g U/g of solution), but no density corrections were needed since all the samples (including the samples used for calibration) were in the same

  13. Units of analysis and kinetic structure of behavioral repertoires.

    Science.gov (United States)

    Thompson, T; Lubinski, D

    1986-09-01

    It is suggested that molar streams of behavior are constructed of various arrangements of three elementary constituents (elicited, evoked, and emitted response classes). An eight-cell taxonomy is elaborated as a framework for analyzing and synthesizing complex behavioral repertoires based on these functional units. It is proposed that the local force binding functional units into a smoothly articulated kinetic sequence arises from temporally arranged relative response probability relationships. Behavioral integration is thought to reflect the joint influence of the organism's hierarchy of relative response probabilities, fluctuating biological states, and the arrangement of environmental and behavioral events in time.

  14. Thermal Hydraulic Analysis of K-DEMO Single Blanket Module for Preliminary Accident Analysis using MELCOR

    Energy Technology Data Exchange (ETDEWEB)

    Moon, Sung Bo; Bang, In Cheol [UNIST, Ulsan (Korea, Republic of)

    2016-05-15

    To develop the Korean fusion commercial reactor, preliminary design concept for K-DEMO (Korean fusion demonstration reactor) has been announced by NFRI (National Fusion Research Institute). This pre-conceptual study of K-DEMO has been introduced to identify technical details of a fusion power plant for the future commercialization of fusion reactor in Korea. Before this consideration, to build the K-DEMO, accident analysis is essential. Since the Fukushima accident, which is severe accident from unexpected disaster, safety analysis of nuclear power plant has become important. The safety analysis of both fission and fusion reactors is deemed crucial in demonstrating the low radiological effect of these reactors on the environment, during severe accidents. A risk analysis of K-DEMO should be performed, as a prerequisite for the construction of a fusion reactor. In this research, thermal-hydraulic analysis of single blanket module of K-DEMO is conducted for preliminary accident analysis for K-DEMO. Further study about effect of flow distributer is conducted. The normal K-DEMO operation condition is applied to the boundary condition and simulated to verify the material temperature limit using MELCOR. MELCOR is fully integrated, relatively fast-running code developed by Sandia National Laboratories. MELCOR had been used for Light Water Reactors and fusion reactor version of MELCOR was developed for ITER accident analysis. This study shows the result of thermal-hydraulic simulation of single blanket module with MELCOR which is severe accident code for nuclear fusion safety analysis. The difference of mass flow rate for each coolant channel with or without flow distributer is presented. With flow distributer, advantage of broadening temperature gradient in the K-DEMO blanket module and increase mass flow toward first wall is obtained. This can enhance the safety of K-DEMO blanket module. Most 13 .deg. C temperature difference in blanket module is obtained.

  15. CRYSTALLIZATION KINETICS OF GLASS-CERAMICS BY DIFFERENTIAL THERMAL ANALYSIS

    Directory of Open Access Journals (Sweden)

    A. NOZAD

    2011-06-01

    Full Text Available The crystallization behavior of fluorphlogopite, a glass-ceramic in the MgO–SiO2–Al2O3–K2O–B2O3–F system, was studied by substitution of Li2O for K2O in the glass composition. DTA, XRD and SEM were used for the study of crystallization behavior, formed phases and microstructure of the resulting glass-ceramics. Crystallization kinetics of the glass was investigated under non-isothermal conditions, using the formal theory of transformations for heterogeneous nucleation. The crystallization results were analyzed, and both the activation energy of crystallization process as well as the crystallization mechanism were characterized. Calculated kinetic parameters indicated that the appropriate crystallization mechanism was bulk crystallization for base glass and the sample with addition of Li2O. Non-isothermal DTA experiments showed that the crystallization activation energies of base glasses was in the range of 234-246 KJ/mol and in the samples with addition of Li2O was changed to the range of 317-322 KJ/mol.

  16. DURIP: Electrokinetic Injection and Separation System for Analysis of Protein and Peptide Transport, Adsorption and Kinetics Instrumentation Proposal

    Science.gov (United States)

    2015-03-18

    are investigating fundamental protein transport , adsorption, and kinetic interactions using a novel nanofluidics-based platform. Since nanofluidic... Protein and Peptide Transport , Adsorption and Kinetics Instrumentation Proposal The views, opinions and/or findings contained in this report are those of...Injection and Separation System for Analysis of Protein and Peptide Transport , Adsorption and Kinetics Instrumentation Proposal Report Title We requested

  17. Cusum charts for preliminary analysis of individual observations

    OpenAIRE

    1997-01-01

    textabstractA preliminary Cusum chart based on individual observations is developed from the uniformly most powerful test for the detection of linear trends. This Cusum chart is compared with several of its competitors which are based on the likelihood ratio test and on transformations of standardized recursive residuals on which for instance the Q-chart methodology is based. It turns out that the new proposed Cusum chart is not only superior in the detection of linear trend out-of-control co...

  18. Grid-connected ICES preliminary feasibility analysis and evaluation. Final report. Volume I. Executive summary

    Energy Technology Data Exchange (ETDEWEB)

    1977-06-30

    A group of hospitals, clinics, research facilities, and medical education facilities, known as the HEAL Complex, was chosen as the site (in New Orleans) for the demonstration of a Grid-Connected Integrated Community Energy System (ICES). The contract work included a preliminary energy supply/demand assessment of the Demonstration Community, a preliminary feasibility analysis and conceptual design of a candidate Demonstration System, preliminary assessment of institutional factors, preparation of a detailed work management plan for subsequent phases of the demonstration program, firming-up of commitments from participating parties, and reporting thereon. This Phase I study has indicated that a central ICES plant producing steam, chilled water, and by-product electricity to serve the HEAL Complex is technically and economically feasible to the extent that Phase II, Detailed Feasibility and Preliminary Design, should be implemented. (MCW)

  19. Kinetics of NH(4) Assimilation in Zea mays: Preliminary Studies with a Glutamate Dehydrogenase (GDH1) Null Mutant.

    Science.gov (United States)

    Magalhães, J R; Ju, G C; Rich, P J; Rhodes, D

    1990-10-01

    In higher plants it is now generally considered that glutamate dehydrogenase (GDH) plays only a small or negligible role in ammonia assimilation. To test this specific point, comparative studies of (15)NH(4) (+) assimilation were undertaken with a GDH1-null mutant of Zea mays and a related (but not strictly isogenic) GDH1-positive wild type from which this mutant was derived. The kinetics of (15)NH(4) (+) assimilation into free amino acids and total reduced nitrogen were monitored in both roots and shoots of 2-week-old seedlings supplied with 5 millimolar 99% ((15)NH(4))(2)SO(4) via the aerated root medium in hydroponic culture over a 24-h period. The GDH1-null mutant, with a 10- to 15-fold lower total root GDH activity in comparison to the wild type, was found to exhibit a 40 to 50% lower rate of (15)NH(4) (+) assimilation into total reduced nitrogen. Observed rates of root ammonium assimilation were 5.9 and 3.1 micromoles per hour per gram fresh weight for the wild type and mutant, respectively. The lower rate of (15)NH(4) (+) assimilation in the mutant was associated with lower rates of labeling of several free amino acids (including glutamate, glutamine-amino N, aspartate, asparagine-amino N, and alanine) in both roots and shoots of the mutant in comparison to the wild type. Qualitatively, these labeling kinetics appear consistent with a reduced flux of (15)N via glutamate in the GDH1-null mutant. However, the responses of the two genotypes to the potent inhibitor of glutamine synthetase, methionine sulfoximine, and differences in morphology of the two genotypes (particularly a lower shoot:root ratio in the GDH1-null mutant) urge caution in concluding that GDH1 is solely responsible for these differences in ammonia assimilation rate.

  20. Kinetic modeling and sensitivity analysis of plasma-assisted combustion

    Science.gov (United States)

    Togai, Kuninori

    Plasma-assisted combustion (PAC) is a promising combustion enhancement technique that shows great potential for applications to a number of different practical combustion systems. In this dissertation, the chemical kinetics associated with PAC are investigated numerically with a newly developed model that describes the chemical processes induced by plasma. To support the model development, experiments were performed using a plasma flow reactor in which the fuel oxidation proceeds with the aid of plasma discharges below and above the self-ignition thermal limit of the reactive mixtures. The mixtures used were heavily diluted with Ar in order to study the reactions with temperature-controlled environments by suppressing the temperature changes due to chemical reactions. The temperature of the reactor was varied from 420 K to 1250 K and the pressure was fixed at 1 atm. Simulations were performed for the conditions corresponding to the experiments and the results are compared against each other. Important reaction paths were identified through path flux and sensitivity analyses. Reaction systems studied in this work are oxidation of hydrogen, ethylene, and methane, as well as the kinetics of NOx in plasma. In the fuel oxidation studies, reaction schemes that control the fuel oxidation are analyzed and discussed. With all the fuels studied, the oxidation reactions were extended to lower temperatures with plasma discharges compared to the cases without plasma. The analyses showed that radicals produced by dissociation of the reactants in plasma plays an important role of initiating the reaction sequence. At low temperatures where the system exhibits a chain-terminating nature, reactions of HO2 were found to play important roles on overall fuel oxidation. The effectiveness of HO2 as a chain terminator was weakened in the ethylene oxidation system, because the reactions of C 2H4 + O that have low activation energies deflects the flux of O atoms away from HO2. For the

  1. Molecular kinetic analysis of a local equilibrium Carnot cycle

    Science.gov (United States)

    Izumida, Yuki; Okuda, Koji

    2017-07-01

    We identify a velocity distribution function of ideal gas particles that is compatible with the local equilibrium assumption and the fundamental thermodynamic relation satisfying the endoreversibility. We find that this distribution is a Maxwell-Boltzmann distribution with a spatially uniform temperature and a spatially varying local center-of-mass velocity. We construct the local equilibrium Carnot cycle of an ideal gas, based on this distribution, and show that the efficiency of the present cycle is given by the endoreversible Carnot efficiency using the molecular kinetic temperatures of the gas. We also obtain an analytic expression of the efficiency at maximum power of our cycle under a small temperature difference. Our theory is also confirmed by a molecular dynamics simulation.

  2. Decoupled direct method for sensitivity analysis in combustion kinetics

    Science.gov (United States)

    Radhakrishnan, Krishnan

    1987-01-01

    An efficient, decoupled direct method for calculating the first order sensitivity coefficients of homogeneous, batch combustion kinetic rate equations is presented. In this method the ordinary differential equations for the sensitivity coefficients are solved separately from , but sequentially with, those describing the combustion chemistry. The ordinary differential equations for the thermochemical variables are solved using an efficient, implicit method (LSODE) that automatically selects the steplength and order for each solution step. The solution procedure for the sensitivity coefficients maintains accuracy and stability by using exactly the same steplengths and numerical approximations. The method computes sensitivity coefficients with respect to any combination of the initial values of the thermochemical variables and the three rate constant parameters for the chemical reactions. The method is illustrated by application to several simple problems and, where possible, comparisons are made with exact solutions and those obtained by other techniques.

  3. Crystallization kinetics of poly-(lactic acid) with and without talc: Optical microscopy and calorimetric analysis

    Science.gov (United States)

    Refaa, Z.; Boutaous, M.; Rousset, F.; Fulchiron, R.; Zinet, M.; Xin, S.; Bourgin, P.

    2014-05-01

    Poly-(lactic acid) or PLA is a biodegradable polymer synthesized from renewable resources. Recently, the discovery of new polymerization routes has allowed increasing the produced volumes. As a consequence, PLA is becoming of great interest for reducing the dependence on petroleum-based plastics. Because of its interesting mechanical properties, PLA is seen as a potential substitute for some usual polymers. However, its relatively slow crystallization kinetics can be a disadvantage with regard to industrial applications. The crystallization kinetics of PLA can be enhanced by adding nucleating agents, which also influences on crystalline morphology and rheological behavior. In the present work, the isothermal quiescent crystallization kinetics of both neat PLA and PLA/talc composite (5 wt% talc) are investigated. The effects of talc on the overall crystallization kinetics and on the crystalline morphology are analyzed using both optical microscopy measurements and thermal analysis by differential scanning calorimetry.

  4. Turbine Fuels from Tar Sands Bitumen and Heavy Oil. Phase I. Preliminary Process Analysis.

    Science.gov (United States)

    1985-04-09

    Process Analysis A. F. Talbot. V. Elanchenny, L. H. Finkel, A. Macris and 3. P. Schwedock Sun Tech, Inc., A Subsidiary of Sun Co. P. 0. Box 1135 Marcus Hook...investigation be carried out in three discrete phases, as described below: Phase I - Preliminary process analysis includes an eval- uation of the potential of

  5. A Preliminary Study on Gender Differences in Studying Systems Analysis and Design

    Science.gov (United States)

    Lee, Fion S. L.; Wong, Kelvin C. K.

    2017-01-01

    Systems analysis and design is a crucial task in system development and is included in a typical information systems programme as a core course. This paper presented a preliminary study on gender differences in studying a systems analysis and design course of an undergraduate programme. Results indicated that male students outperformed female…

  6. Evaluation of kinetic entropy of breast masses initially found on MRI using whole-lesion curve distribution data: Comparison with the standard kinetic analysis

    Energy Technology Data Exchange (ETDEWEB)

    Shimauchi, Akiko [University of Chicago, Department of Radiology, Chicago, IL (United States); Tohoku University, Department of Diagnostic Radiology, Graduate School of Medicine, Sendai, Miyagi (Japan); Abe, Hiroyuki; Schacht, David V.; Yulei, Jian; Pineda, Federico D.; Jansen, Sanaz A.; Ganesh, Rajiv; Newstead, Gillian M. [University of Chicago, Department of Radiology, Chicago, IL (United States)

    2015-08-15

    To quantify kinetic heterogeneity of breast masses that were initially detected with dynamic contrast-enhanced MRI, using whole-lesion kinetic distribution data obtained from computer-aided evaluation (CAE), and to compare that with standard kinetic curve analysis. Clinical MR images from 2006 to 2011 with breast masses initially detected with MRI were evaluated with CAE. The relative frequencies of six kinetic patterns (medium-persistent, medium-plateau, medium-washout, rapid-persistent, rapid-plateau, rapid-washout) within the entire lesion were used to calculate kinetic entropy (KE), a quantitative measure of enhancement pattern heterogeneity. Initial uptake (IU) and signal enhancement ratio (SER) were obtained from the most-suspicious kinetic curve. Mann-Whitney U test and ROC analysis were conducted for differentiation of malignant and benign masses. Forty benign and 37 malignant masses comprised the case set. IU and SER were not significantly different between malignant and benign masses, whereas KE was significantly greater for malignant than benign masses (p = 0.748, p = 0.083, and p < 0.0001, respectively). Areas under ROC curve for IU, SER, and KE were 0.479, 0.615, and 0.662, respectively. Quantification of kinetic heterogeneity of whole-lesion time-curve data with KE has the potential to improve differentiation of malignant from benign breast masses on breast MRI. (orig.)

  7. Preliminary findings on Bagaza virus (Flavivirus: Flaviviridae growth kinetics, transmission potential & transovarial transmission in three species of mosquitoes

    Directory of Open Access Journals (Sweden)

    A B Sudeep

    2013-01-01

    Full Text Available Background & objectives: Bagaza virus (BAGV, a flavivirus synonymous with Israel turkey meningoencephalitis virus, has been found to circulate in India. BAGV has recently been held responsible for inducing febrile illness in humans and causing unusually high mortality to wild birds in Spain. A study was therefore, undertaken to determine its replication kinetics in certain mosquitoes and to determine vector competence and potential of the mosquitoes to transmit BAGV experimentally. Methods: Aedes aegypti, Culex tritaeniorhynchus and Cx quinquefasciatus mosquitoes were inoculated with BAGV; samples were harvested every day and titrated in BHK-21 cell line. Vector competence and experimental transmission were determined by examining the saliva of infected mosquitoes for virus and induction of sickness in suckling mice, respectively. Results: Cx. tritaeniorhynchus and Ae. aegypti mosquitoes yielded 5 log 10 and 4.67 log 10 TCID 50 /ml of virus on day 3 post-infection (PI, respectively while Cx. quinquefasciatus yielded a titre of 4 log 10 TCID 50 /ml on day 4 PI. BAGV was detected in saliva of all the infected mosquitoes demonstrating their vector competence. Experimental transmission of BAGV to infant mice as well as transovarial transmission was demonstrated by Cx. tritaeniorhynchus but not by Ae. aegypti and Cx. quinquefasciatus mosquitoes. Interpretation & conclusions: Replication of BAGV to high titres and dissemination to saliva in three most prevalent mosquitoes in India is of immense public health importance. Though no major outbreak involving man has been reported yet, BAGV has a potential to cause outbreaks in future.

  8. Marking with radioactive iodine of a plasma substitute and preliminary essays of his kinetic behaviour in rats; Marcacao com radioiodo de um sucedaneo do plasma e ensaios preliminares de seu comportamento cinetico em ratos

    Energy Technology Data Exchange (ETDEWEB)

    Cova, Wilma Guimaraes

    1973-07-01

    A blood plasma substitute (Haem accel - PGO), which has as a base a degraded and polymerized gelatin, was labelled with radioactive iodine (I-131) and preliminary essays of its kinetic behavior was done. We have used the labelling method of McFarlaner. The results obtained - radiochemical yield and purity - were favorable. The kinetic behaviour the residual radioactivity studied by measuring the residual radioactivity of the body and excreta in groups of male Wistar rats for a maximum period of 150 hours. These results have shown and excellent correlation for a two components exponential function's adjustment, suggesting therefore a bi compartmental mathematical model. (author)

  9. Preliminary Analysis of HL-2A Global Energy Confinement

    Institute of Scientific and Technical Information of China (English)

    DONG Chunfeng; CUI Zhengying; JI Xiaoquan; ZHOU Hangyu; FENG Beibin; SUN Hongjuan; LI Yonggao; YANG Qingwei

    2009-01-01

    Energy confinement time taken from 135 discharges of the 2006 campaign in HL-2A is studied.The.data obtained from the measurements are verified by comparing diamagnetic energy with the electronic kinetic energy calculated from both the electron temperature and density profiles.Two data sets for supporting the ITER L-Mode confinement database are generated from the 2006 campaign.The dependence of гE on the line-averaged electron density during ohmic phases is analysed.The comparison of гE in electron cyclotron resonance heating(ECRH) plasma as well as the existing ITER L-mode scalings is made.The results show that the energy confinement time is consistent with the ITER L-mode scalings.

  10. Kinetic analysis of the lower limbs in baseball tee batting.

    Science.gov (United States)

    Ae, Kazumichi; Koike, Sekiya; Fujii, Norihisa; Ae, Michiyoshi; Kawamura, Takashi

    2017-09-01

    The purpose of this study was to investigate effects of the ground reaction forces on the rotation of the body as a whole and on the joint torques of the lower limbs associated with trunk and pelvic rotation in baseball tee batting. A total of 22 male collegiate baseball players participated in this study. Three-dimensional coordinate data were acquired by a motion capture system (250 Hz), and ground reaction forces of both legs were measured with three force platforms (1,000 Hz). Kinetic data were used to calculate the moment about the vertical axis through the body's centre of mass resulting from ground reaction forces, as well as to calculate the torque and mechanical work in the lower limb joints. The lateral/medial ground reaction force generated by both legs resulted in the large whole body moment about its vertical axis. The joint torques of flexion/extension of both hips, adduction of the stride hip and extension of the stride knee produced significantly larger mechanical work than did the other joint torques. To obtain high bat-head speed, the batter should push both legs in the lateral/medial direction by utilising both hips and stride knee torques so as to increase the whole body rotation.

  11. Multisteps Global Kinetic Analysis of MSW Slow Pyrolysis

    Directory of Open Access Journals (Sweden)

    Dwi Aries Himawanto

    2013-12-01

    Full Text Available The goal of this research is to find relationships between single components slow pyrolysis characteristics and mixed component slow pyrolysis characteristics of segregated municipal solid wastes (MSW. The material of this research consists of organic wastes (bamboo wastes and banana leaves wastes and inorganic wastes (styrofoam wastes and snack wrapping wastes. The materials which used to study were the unprosessing waste. The samples were collected, dried and crushed until passing 20 mesh shieves then characterized in self manufactured macro balance. The thermogravimetry analyses were done to find the MSW slow pyrolysis characteristics. The 20 gram sample was placed in the furnace whose temperature is increased with 10 0C/min heating rate until reached 400 0 final temperature and held for 30 minutes before the sample is cooled into room temperature. One hundred ml/min nitrogen introduced from the bottom of furnace as a swept gas. The results of the research show that the global kinetic method could be used to predict the MSW single component activation energy but it should be modified to calculate the mixed sample activation energy . The predictive activation energy values which calculated based on weighed sum of single component have 18.5 % deviations if compared with experimental result.

  12. Analysis of kinetic Langmuir model. Part I: Integrated kinetic Langmuir equation (IKL): a new complete analytical solution of the Langmuir rate equation.

    Science.gov (United States)

    Marczewski, Adam W

    2010-10-05

    In the article, a new integrated kinetic Langmuir equation (IKL) is derived. The IKL equation is a simple and easy to analyze but complete analytical solution of the kinetic Langmuir model. The IKL is compared with the nth-order, mixed 1,2-order, and multiexponential kinetic equations. The impact of both equilibrium coverage θ(eq) and relative equilibrium uptake u(eq) on kinetics is explained. A newly introduced Langmuir batch equilibrium factor f(eq) that is the product of both parameters θ(eq)u(eq) is used to determine the general kinetic behavior. The analysis of the IKL equation allows us to understand fully the Langmuir kinetics and explains its relation with respect to the empirical pseudo-first-order (PFO, i.e., Lagergren), pseudo-second-order (PSO), and mixed 1,2-order kinetic equations, and it shows the conditions of their possible application based on the Langmuir model. The dependence of the initial adsorption rate on the system properties is analyzed and compared to the earlier published approximate equations.

  13. Thick Concrete Specimen Construction, Testing, and Preliminary Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Clayton, Dwight A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Hoegh, Kyle [Univ. of Minnesota, Minneapolis, MN (United States); Khazanovich, Lev [Univ. of Minnesota, Minneapolis, MN (United States)

    2015-03-01

    The purpose of the U.S. Department of Energy Office of Nuclear Energy’s Light Water Reactor Sustainability (LWRS) Program is to develop technologies and other solutions that can improve the reliability, sustain the safety, and extend the operating lifetimes of nuclear power plants (NPPs) beyond 60 years. Since many important safety structures in an NPP are constructed of concrete, inspection techniques must be developed and tested to evaluate the internal condition. In-service containment structures generally do not allow for the destructive measures necessary to validate the accuracy of these inspection techniques. This creates a need for comparative testing of the various nondestructive evaluation (NDE) measurement techniques on concrete specimens with known material properties, voids, internal microstructure flaws, and reinforcement locations. A preliminary report detailed some of the challenges associated with thick reinforced concrete sections and prioritized conceptual designs of specimens that could be fabricated to represent NPP concrete structures for using in NDE evaluation comparisons. This led to the construction of the concrete specimen presented in this report, which has sufficient reinforcement density and cross-sectional size to represent an NPP containment wall. Details on how a suitably thick concrete specimen was constructed are presented, including the construction materials, final nominal design schematic, as well as formwork and rigging required to safely meet the desired dimensions of the concrete structure. The report also details the type and methods of forming the concrete specimen as well as information on how the rebar and simulated defects were embedded. Details on how the resulting specimen was transported, safely anchored, and marked to allow access for systematic comparative NDE testing of defects in a representative NPP containment wall concrete specimen are also given. Data collection using the MIRA Ultrasonic NDE equipment and

  14. In vitro comparative kinetic analysis of the chloroplast Toc GTPases.

    Science.gov (United States)

    Reddick, L Evan; Vaughn, Michael D; Wright, Sarah J; Campbell, Ian M; Bruce, Barry D

    2007-04-13

    A unique aspect of protein transport into plastids is the coordinate involvement of two GTPases in the translocon of the outer chloroplast membrane (Toc). There are two subfamilies in Arabidopsis, the small GTPases (Toc33 and Toc34) and the large acidic GTPases (Toc90, Toc120, Toc132, and Toc159). In chloroplasts, Toc34 and Toc159 are implicated in precursor binding, yet mechanistic details are poorly understood. How the GTPase cycle is modulated by precursor binding is complex and in need of careful dissection. To this end, we have developed novel in vitro assays to quantitate nucleotide binding and hydrolysis of the Toc GTPases. Here we present the first systematic kinetic characterization of four Toc GTPases (cytosolic domains of atToc33, atToc34, psToc34, and the GTPase domain of atToc159) to permit their direct comparison. We report the KM, Vmax, and Ea values for GTP hydrolysis and the Kd value for nucleotide binding for each protein. We demonstrate that GTP hydrolysis by psToc34 is stimulated by chloroplast transit peptides; however, this activity is not stimulated by homodimerization and is abolished by the R133A mutation. Furthermore, we show peptide stimulation of hydrolytic rates are not because of accelerated nucleotide exchange, indicating that transit peptides function as GTPase-activating proteins and not guanine nucleotide exchange factors in modulating the activity of psToc34. Finally, by using the psToc34 structure, we have developed molecular models for atToc33, atToc34, and atToc159G. By combining these models with the measured enzymatic properties of the Toc GTPases, we provide new insights of how the chloroplast protein import cycle may be regulated.

  15. Analysis of drying potato kinetics in laboratory conditions

    Directory of Open Access Journals (Sweden)

    Zlatković B.P.

    2005-01-01

    Full Text Available Chemistry is in its nature a fundamental science, but nowadays its results are increasingly used in practice. In such a way, many syntheses of important nutrition and industrial compounds (chemical technology, or destruction of harmful substances (chemical ecology have been developed. The analytic part of chemistry (analytical chemistry has found a significant usage even in optimization of technological processes of food technology. One of the oldest ways of food preservation is drying (dehydration. The basis of this process of preservation is to vaporize water, to dry enough the product, in order to stop the activity of enzymes and possibility of microorganisms’ development. Water in agricultural products is not free but bound to dry matter. Therefore, for its vaporizing it is necessary to bring enough heat. The part of the brought heat is used for vaporizing water, but one part of it becomes the energy for activation of several chemical reactions that decrease the nutritive values and the quality of future food. Therefore, the important engineering problem emerges: determination of optimal conditions for drying. For optimization of technological process of drying, it is necessary to do mathematical modeling of dependence of water vaporizing speed from environmental conditions beforehand. The aim of this paper is to determine potato pieces drying kinetics in laboratory dryer, in order to determine the optimal conditions for its industrial dehydration. It was established that in the first quarter of the time of drying evaporated 28.3% of total evaporation of water, and then in order by quarters: 53%, 14.1% and 4.6%, respectively. The greatest speed of drying was realized at moisture of potatoes about 50%.

  16. Heat Transfer Analysis and Assessment of Kinetics Systems for PBX 9501

    Energy Technology Data Exchange (ETDEWEB)

    Jorenby, Jeffrey W. [Colorado State Univ., Fort Collins, CO (United States)

    2006-07-01

    The study of thermal decomposition in high explosive (HE) charges has been an ongoing process since the early 1900s. This work is specifically directed towards the analysis of PBX 9501. In the early 1970s, Dwight Jaeger of Los Alamos National Laboratory (LANL) developed a single-step, two-species kinetics system that was used in the development of one of the first finite element codes for thermal analyses known as EXPLO. Jaeger's research focused on unconfined spherical samples of HE charges to determine if varied heating ramps would cause detonation or deflagration. Tarver and McGuire of Lawrence Livermore National Laboratory (LLNL) followed soon after with a three-step, four-species kinetics system that was developed for confined spheres under relatively fast heating conditions. Peter Dickson et al. of LANL then introduced a kinetics system with four steps and five species that included bimolecular products to capture the effects of the endothermic phase change that the HE undergoes. The results of four experiments are examined to study the effectiveness of these kinetics systems. The experiments are: (1) The LLNL scaled thermal explosion (STEX) experiments on confined cylindrical charges with long heating ramps in the range of 90 hours. (2) The LLNL one-dimensional time to explosion (ODTX) experiments on spherical charges that include confined, partially confined, and aged HE experiments. (3) The LANL unconfined one-dimensional experiments for small spheres. (4) The Naval Air Warfare Center Weapons Division at China Lake experiments on small confined cylinders. The three kinetics systems are applied to each of the four experiments with the use of the finite element analysis (FEA) heat conduction solver COYOTE. The numerical results using the kinetics systems are compared to each other and to the experimental data to determine which kinetics systems are best suited for analyzing conditions such as time to ignition, containment, heating time, and location of

  17. Preliminary Analysis of Species Partitioning in the DWPF Melter

    Energy Technology Data Exchange (ETDEWEB)

    Choi, A. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Kesterson, M. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Johnson, F. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); McCabe, D. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2015-07-15

    The work described in this report is preliminary in nature since its goal was to demonstrate the feasibility of estimating the off-gas entrainment rates from the Defense Waste Processing Facility (DWPF) melter based on a simple mass balance using measured feed and glass pour stream compositions and timeaveraged melter operating data over the duration of one canister-filling cycle. The only case considered in this study involved the SB6 pour stream sample taken while Canister #3472 was being filled over a 20-hour period on 12/20/2010, approximately three months after the bubblers were installed. The analytical results for that pour stream sample provided the necessary glass composition data for the mass balance calculations. To estimate the “matching” feed composition, which is not necessarily the same as that of the Melter Feed Tank (MFT) batch being fed at the time of pour stream sampling, a mixing model was developed involving three preceding MFT batches as well as the one being fed at that time based on the assumption of perfect mixing in the glass pool but with an induction period to account for the process delays involved in the calcination/fusion step in the cold cap and the melter turnover.

  18. Preliminary Coupling of MATRA Code for Multi-physics Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Seongjin; Choi, Jinyoung; Yang, Yongsik; Kwon, Hyouk; Hwang, Daehyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-05-15

    The boundary conditions such as the inlet temperature, mass flux, averaged heat flux, power distributions of the rods, and core geometry is given by constant values or functions of time. These conditions are separately calculated and provided by other codes, such as a neutronics or a system codes, into the MATRA code. In addition, the coupling of several codes in the different physics field is focused and embodied. In this study, multiphysics coupling methods were developed for a subchannel code (MATRA) with neutronics codes (MASTER, DeCART) and a fuel performance code (FRAPCON-3). Preliminary evaluation results for representative sample cases are presented. The MASTER and DeCART codes provide the power distribution of the rods in the core to the MATRA code. In case of the FRAPCON-3 code, the variation of the rod diameter induced by the thermal expansion is yielded and provided. The MATRA code transfers the thermal-hydraulic conditions that each code needs. Moreover, the coupling method with each code is described.

  19. Laboratory Investigations on Estuary Salinity Mixing: Preliminary Analysis

    Directory of Open Access Journals (Sweden)

    F. H. Nuryazmeen

    2014-05-01

    Full Text Available Estuaries are bodies of water along the coasts that are formed when fresh water from rivers flows into and mixes with salt water from the ocean. The estuaries serve as a habitat to some aquatic lives, including mangroves. Human-induced activities such as dredging of shipping lanes along the bottom estuarine, the disposal of industrial wastes into the water system and shoreline development influence estuarine dynamics which include mixing process. These activities might contribute to salinity changes and further adversely affect the estuarine ecosystem. In order to study at the characteristics of the mixing between salt water (estuary and freshwater (river, a preliminary investigation had been done in the laboratory. Fresh water was released from one end of the flume and overflowing at weir at the other end. Meanwhile, salt water was represented by the red dye tracer released through a weir and intruded upstream as a gravity current. The isohalines are plotted to see the salinity patterns. Besides, to examine the spatial and temporal salinity profiles along the laboratory investigations, the plotted graphs have been made. The results show that the changes in salinity level along the flume due to mixing between fresh water and salt water. This showed typical salt-wedge estuary characteristics.

  20. Fukushima Daiichi unit 1 uncertainty analysis--Preliminary selection of uncertain parameters and analysis methodology

    Energy Technology Data Exchange (ETDEWEB)

    Cardoni, Jeffrey N.; Kalinich, Donald A.

    2014-02-01

    Sandia National Laboratories (SNL) plans to conduct uncertainty analyses (UA) on the Fukushima Daiichi unit (1F1) plant with the MELCOR code. The model to be used was developed for a previous accident reconstruction investigation jointly sponsored by the US Department of Energy (DOE) and Nuclear Regulatory Commission (NRC). However, that study only examined a handful of various model inputs and boundary conditions, and the predictions yielded only fair agreement with plant data and current release estimates. The goal of this uncertainty study is to perform a focused evaluation of uncertainty in core melt progression behavior and its effect on key figures-of-merit (e.g., hydrogen production, vessel lower head failure, etc.). In preparation for the SNL Fukushima UA work, a scoping study has been completed to identify important core melt progression parameters for the uncertainty analysis. The study also lays out a preliminary UA methodology.

  1. Kinetic Analysis of Haloacetonitrile Stability in Drinking Waters.

    Science.gov (United States)

    Yu, Yun; Reckhow, David A

    2015-09-15

    Haloacetonitriles (HANs) are an important class of drinking water disinfection byproducts (DBPs) that are reactive and can undergo considerable transformation on time scales relevant to system distribution (i.e., from a few hours to a week or more). The stability of seven mono-, di-, and trihaloacetonitriles was examined under a variety of conditions including different pH levels and disinfectant doses that are typical of drinking water distribution systems. Results indicated that hydroxide, hypochlorite, and their protonated forms could react with HANs via nucleophilic attack on the nitrile carbon, forming the corresponding haloacetamides (HAMs) and haloacetic acids (HAAs) as major reaction intermediates and end products. Other stable intermediate products, such as the N-chloro-haloacetamides (N-chloro-HAMs), may form during the course of HAN chlorination. A scheme of pathways for the HAN reactions was proposed, and the rate constants for individual reactions were estimated. Under slightly basic conditions, hydroxide and hypochlorite are primary reactants and their associated second-order reaction rate constants were estimated to be 6 to 9 orders of magnitude higher than those of their protonated conjugates (i.e., neutral water and hypochlorous acid), which are much weaker but more predominant nucleophiles at neutral and acidic pHs. Developed using the estimated reaction rate constants, the linear free energy relationships (LFERs) summarized the nucleophilic nature of HAN reactions and demonstrated an activating effect of the electron withdrawing halogens on nitrile reactivity, leading to decreasing HAN stability with increasing degree of halogenation of the substituents, while subsequent shift from chlorine to bromine atoms has a contrary stabilizing effect on HANs. The chemical kinetic model together with the reaction rate constants that were determined in this work can be used for quantitative predictions of HAN concentrations depending on pH and free chlorine

  2. Preliminary Analysis of a Novel SAR Based Emergency System for Earth Orbit Satellites using Galileo

    NARCIS (Netherlands)

    Gill, E.K.A.; Helderweirt, A.

    2010-01-01

    This paper presents a preliminary analysis of a novel Search and Rescue (SAR) based emergency system for Low Earth Orbit (LEO) satellites using the Galileo Global Navigation Satellite System (GNSS). It starts with a description of the space user SAR system including a concept description, mission ar

  3. ANSI/ASHRAE/IES Standard 90.1-2013 Preliminary Determination: Quantitative Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Halverson, Mark A.; Rosenberg, Michael I.; Wang, Weimin; Zhang, Jian; Mendon, Vrushali V.; Athalye, Rahul A.; Xie, YuLong; Hart, Reid; Goel, Supriya

    2014-03-01

    This report provides a preliminary quantitative analysis to assess whether buildings constructed according to the requirements of ANSI/ASHRAE/IES Standard 90.1-2013 would result in energy savings compared with buildings constructed to ANSI/ASHRAE/IES Standard 90.1-2010.

  4. Critical Analysis of Underground Coal Gasification Models. Part II: Kinetic and Computational Fluid Dynamics Models

    Directory of Open Access Journals (Sweden)

    Alina Żogała

    2014-01-01

    Originality/value: This paper presents state of art in the field of coal gasification modeling using kinetic and computational fluid dynamics approach. The paper also presents own comparative analysis (concerned with mathematical formulation, input data and parameters, basic assumptions, obtained results etc. of the most important models of underground coal gasification.

  5. On the graph and systems analysis of reversible chemical reaction networks with mass action kinetics

    NARCIS (Netherlands)

    Rao, Shodhan; Jayawardhana, Bayu; Schaft, Arjan van der

    2012-01-01

    Motivated by the recent progresses on the interplay between the graph theory and systems theory, we revisit the analysis of reversible chemical reaction networks described by mass action kinetics by reformulating it using the graph knowledge of the underlying networks. Based on this formulation, we

  6. Modelling and simulation of a transketolase mediated reaction: Sensitivity analysis of kinetic parameters

    DEFF Research Database (Denmark)

    Sayar, N.A.; Chen, B.H.; Lye, G.J.

    2009-01-01

    In this paper we have used a proposed mathematical model, describing the carbon-carbon bond format ion reaction between beta-hydroxypyruvate and glycolaldehyde to synthesise L-erythrulose, catalysed by the enzyme transketolase, for the analysis of the sensitivity of the process to its kinetic par....... (C) 2009 Elsevier B.V. All rights reserved....

  7. Thermal degradation kinetics and isoconversional analysis of biodegradable poly(3-hydroxybutyrate)/organomodified montmorillonite nanocomposites

    Energy Technology Data Exchange (ETDEWEB)

    Achilias, Dimitris S., E-mail: axilias@chem.auth.gr [Laboratory of Organic Chemical Technology, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki (Greece); Panayotidou, Elpiniki [Laboratory of Organic Chemical Technology, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki (Greece); Department of Industrial Design Engineering, TEI of Western Macedonia, 50100 Kozani (Greece); Zuburtikudis, Ioannis [Department of Industrial Design Engineering, TEI of Western Macedonia, 50100 Kozani (Greece)

    2011-02-20

    Poly(3-hydroxybutyrate) (PHB)/organically modified clay nanocomposites were prepared by the melt mixing method and were characterized using wide-angle X-ray diffraction. Their thermal degradation kinetics was investigated using thermogravimetric analysis at various heating rates. Further kinetic analysis was performed using isoconversional methods and the invariant kinetic parameters method was used to estimate the so-called 'true' kinetic parameters, i.e. the pre-exponential factor, A and the activation energy, E, as well as the reaction model. It was found that intercalated structures are formed and the thermal stability of the material is improved by the addition of the nano-filler. From the isoconversional analysis, it was found that the activation energy does not vary significantly with the degree of degradation denoting degradation in one step with similar values for pure PHB and for all nanocomposites. Using the invariant kinetic parameters method, it was found that the model that best describes the experimental data was that of Sestak-Berggren's with f(a) = {alpha}{sup n}(1 - {alpha}){sup m}, where the value of n is always larger than m and is increasing with the amount of the nano-filler. The value of the 'true' activation energy was found to be about 100 kJ mol{sup -1} for all nanocomposites and the pre-exponential factor for PHB was estimated equal to 5.35 x 10{sup 9} min{sup -1}. Finally, the values of the kinetic rate constant k were found to decrease with the amount of the nano-filler up to 3 wt%, while for amounts larger than 3 wt% k increased reaching a value greater than that of pure PHB for the 10 wt% nanocomposites.

  8. Preliminary Analysis of the Oklahoma Wavefields Demonstration Dataset

    Science.gov (United States)

    Anderson, K. R.; Sweet, J. R.; Woodward, R.; Karplus, M. S.; DeShon, H. R.; Magnani, M. B.; Hayward, C.; Langston, C. A.

    2016-12-01

    In June 2016, a field crew of 50 students, faculty, industry personnel and IRIS staff deployed a total of 390 stations as part of a community seismic experiment above an active seismic lineament in north-central Oklahoma. The goals of the experiment were to test new instrumentation and deployment strategies that record the full wavefield, and to advance understanding of earthquake source processes and regional lithospheric structure. The crew deployed 363 3C 4.5Hz Generation 2 Fairfield Z-Land nodes along three seismic lines and in a seven-layer nested gradiometer array. The seismic lines spanned a region 13 km long by 5 km wide. The nested gradiometer was designed to measure the full seismic wavefield using standard frequency-wavenumber techniques and spatial wave gradients. A broadband, 18 station "Golay 3x6" array was deployed around the gradiometer and seismic lines with an aperture of approximately 5 km to collect waveform data from local and regional events. In addition, 9 infrasound stations were deployed in order to capture and identify acoustic events that might be recorded by the seismic arrays and to quantify the wind acoustic noise effect on co-located broadband stations. The variety of instrumentation used in this deployment was chosen to capture the full seismic wavefield generated by the local and regional seismicity beneath the array and the surrounding region. We present preliminary results from the data collected during the experiment. We analyze the level of signal coherence observed across the nested gradiometer and Golay array as well as array design fidelity. We report on data quality, including completeness and noise levels, for the various types of instrumentation. We also examine the performance of co-located surface and buried nodes to determine the benefits of each installation type. Finally, we present performance comparisons between co-located nodes and broadband stations and compare these results to prior wavefield/large-N deployments

  9. Preliminary analysis techniques for ring and stringer stiffened cylindrical shells

    Science.gov (United States)

    Graham, J.

    1993-03-01

    This report outlines methods of analysis for the buckling of thin-walled circumferentially and longitudinally stiffened cylindrical shells. Methods of analysis for the various failure modes are presented in one cohesive package. Where applicable, more than one method of analysis for a failure mode is presented along with standard practices. The results of this report are primarily intended for use in launch vehicle design in the elastic range. A Microsoft Excel worksheet with accompanying macros has been developed to automate the analysis procedures.

  10. Experimental study and kinetic analysis of the oxidation of light hydrocarbons mixtures

    Energy Technology Data Exchange (ETDEWEB)

    Rota, R.; Bonini, F.; Morbidelli, M.; Carra, S. [Politecnico di Milano (Italy). Dipt. di Chimica Fisica Applicata

    1996-07-01

    The combustion of various C{sup 1}-C{sub 2} hydrocarbon mixtures has been experimentally investigated in a continuous perfectly stirred reactor, in a temperature range from 1,000 to 1,300 K, and at stoichiometric ratio values between 0.5 and 1.5. The concentration values of several molecular species have been measured by GC analysis. The data obtained have been compared with the predictions of two different detailed kinetic models; both fail to predict the experimental trends in various situations. This leads to the conclusion that experimental data based on mixtures of hydrocarbons must be obtained to validate detailed kinetic mechanisms.

  11. Kinetic Analysis for Macrocyclizations Involving Anionic Template at the Transition State

    Directory of Open Access Journals (Sweden)

    Vicente Martí-Centelles

    2012-01-01

    competitive oligomerization/polymerization processes yielding undesired oligomeric/polymeric byproducts. The effect of anions has also been included in the kinetic models, as they can act as catalytic templates in the transition state reducing and stabilizing the transition state. The corresponding differential equation systems for each kinetic model can be solved numerically. Through a comprehensive analysis of these results, it is possible to obtain a better understanding of the different parameters that are involved in the macrocyclization reaction mechanism and to develop strategies for the optimization of the desired processes.

  12. A kinetic analysis of strand breaks on large DNA induced by cigarette smoke extract

    Science.gov (United States)

    Kurita, Hirofumi; Takata, Tatsuya; Yasuda, Hachiro; Takashima, Kazunori; Mizuno, Akira

    2010-06-01

    We report a kinetic analysis of strand breakages on large DNA molecules induced by cigarette smoke extract (CSE), an extract of soluble cigarette smoke components. Previously, this DNA damage was analyzed by agarose gel electrophoresis, whereas we used fluorescence to kinetically analyze damage to individual DNA molecules. CSE caused a marked change in length of DNA molecules. The rate of CSE-induced double-strand breakage on large random-coiled DNA molecules was determined using a simple theoretical model, allowing the facile estimation of the rate of double-strand breaks on large DNA molecules.

  13. Non-Isothermal Kinetic Analysis of the Crystallization of Metallic Glasses Using the Master Curve Method

    OpenAIRE

    Jürgen Eckert; Stefan Roth; Joan Torrens-Serra; Mihai Stoica; Uta Kuehn; Shankar Venkataraman

    2011-01-01

    The non-isothermal transformation rate curves of metallic glasses are analyzed with the Master Curve method grounded in the Kolmogorov-Johnson-Mehl-Avrami theory. The method is applied to the study of two different metallic glasses determining the activation energy of the transformation and the experimental kinetic function that is analyzed using Avrami kinetics. The analysis of the crystallization of Cu47Ti33Zr11Ni8Si1 metallic glassy powders gives Ea = 3.8 eV, in good agreement with the cal...

  14. Thermodynamic and Kinetic Analysis of Sensitivity Amplification in Biological Signal Transduction

    CERN Document Server

    Qian, H

    2003-01-01

    Based on a thermodynamic analysis of the kinetic model for the protein phosphorylation-dephosphorylation cycle, we study the ATP (or GTP) energy utilization of this ubiquitous biological signal transduction process. It was shown that the free energy from hydrolysis inside cells, Delta G (phosphorylation potential), controls the amplification and sensitivity of the switch-like cellular module; the response coefficient of the sensitivity amplification approaches the optimal 1 and the Hill coefficeint increases with increasing Delta G. Futhermore, we show the high amplification in zero-order ultrasensitivity is mechanistically related to the proofreading kinetics for protein biosynthesis. Both utilize multiple kinetic cycles in time to gain temporal cooperativity, in contrast to allosteric cooperativity that utilizes multiple subunits in a protein.

  15. A COMPUTERIZED SYSTEM ON KINETIC ANALYSIS AND EVALUATION OF GAS/SOLID REACTIONS

    Institute of Scientific and Technical Information of China (English)

    J.H. Liu; J. Y. Zhang; S.K. Wei

    2003-01-01

    The present paper presents the structure, features and functions of a computerized system on kinetic analysis and evaluation of gas/solid reactions, KinPreGSR. KinPreGSR is a menu driven system, can be operated with MS Windows as workbench in a PC computer. It has been developed using visual C++ with FoxPro hybrid coding technique.KinPreGSR combines the characteristics of gas/solid reactions with the kinetic models as well as mass and heat transfer equations. The database files were established for the apparent activation energies of some reduction and decomposition reactions to allow the prediction of the reaction kinetics to some extents. Outputs can be displayed using graphical or numerical forms. Examples regarding the oxide reduction and carbonate decomposition under isothermal conditions are given to show those functions.

  16. Comparative kinetic analysis of silent and ultrasound-assisted catalytic wet peroxide oxidation of phenol.

    Science.gov (United States)

    Rokhina, Ekaterina V; Repo, Eveliina; Virkutyte, Jurate

    2010-03-01

    The kinetic study of silent and ultrasound-assisted catalytic wet peroxide oxidation of phenol in water was performed to qualitatively assess the effect of ultrasound on the process kinetics. Various kinetic parameters such as the apparent kinetic rate constants, the surface utilization coefficient and activation energy of phenol oxidation over RuI(3) catalyst were investigated. Comparative analysis revealed that the use of ultrasound irradiation reduced the energy barrier of the reaction but had no impact on the reaction pathway. The activation energy for the oxidation of phenol over RuI(3) catalyst in the presence of ultrasound was found to be 13kJmol(-1), which was four times smaller in comparison to the silent oxidation process (57kJmol(-1)). Finally, 'figures-of-merit' was utilized to assess different experimental strategies such as sonolysis alone, H(2)O(2)-enhanced sonolysis and sono-catalytic oxidation of phenol in order to estimate the electric energy consumption based on the kinetic rate constants of the oxidation process.

  17. Preliminary Design and Analysis of ITER In-Wall Shielding

    Institute of Scientific and Technical Information of China (English)

    LIU Changle; YU Jie; WU Songtao; CAI Yingxiang; PAN Wanjiang

    2007-01-01

    ITER in-wall shielding (IIS) is situated between the doubled shells of the ITER Vacuum Vessel (IVV). Its main functions are applied in shielding neutron, gamma-ray and toroidal field ripple reduction. The structure of IIS has been modelled according to the IVV design criteria which has been updated by the ITER team (IT). Static analysis and thermal expansion analysis were performed for the structure. Thermal-hydraulic analysis verified the heat removal capability and resulting temperature, pressure, and velocity changes in the coolant flow. Consequently, our design work is possibly suitable as a reference for IT's updated or final design in its next step.

  18. Preliminary Technical Risk Analysis for the Geothermal Technologies Program

    Energy Technology Data Exchange (ETDEWEB)

    None

    2009-01-18

    This report explains the goals, methods, and results of a probabilistic analysis of technical risk for a portfolio of R&D projects in the DOE Geothermal Technologies Program (The Program). The analysis is a task by Princeton Energy Resources International, LLC, in support of the National Renewable Energy Laboratory on behalf of the Program. The main challenge in the analysis lies in translating R&D results to a quantitative reflection of technical risk for a key Program metric: levelized cost of energy (LCOE).

  19. First fungal genome sequence from Africa: A preliminary analysis

    Directory of Open Access Journals (Sweden)

    Rene Sutherland

    2012-01-01

    Full Text Available Some of the most significant breakthroughs in the biological sciences this century will emerge from the development of next generation sequencing technologies. The ease of availability of DNA sequence made possible through these new technologies has given researchers opportunities to study organisms in a manner that was not possible with Sanger sequencing. Scientists will, therefore, need to embrace genomics, as well as develop and nurture the human capacity to sequence genomes and utilise the ’tsunami‘ of data that emerge from genome sequencing. In response to these challenges, we sequenced the genome of Fusarium circinatum, a fungal pathogen of pine that causes pitch canker, a disease of great concern to the South African forestry industry. The sequencing work was conducted in South Africa, making F. circinatum the first eukaryotic organism for which the complete genome has been sequenced locally. Here we report on the process that was followed to sequence, assemble and perform a preliminary characterisation of the genome. Furthermore, details of the computer annotation and manual curation of this genome are presented. The F. circinatum genome was found to be nearly 44 million bases in size, which is similar to that of four other Fusarium genomes that have been sequenced elsewhere. The genome contains just over 15 000 open reading frames, which is less than that of the related species, Fusarium oxysporum, but more than that for Fusarium verticillioides. Amongst the various putative gene clusters identified in F. circinatum, those encoding the secondary metabolites fumosin and fusarin appeared to harbour evidence of gene translocation. It is anticipated that similar comparisons of other loci will provide insights into the genetic basis for pathogenicity of the pitch canker pathogen. Perhaps more importantly, this project has engaged a relatively large group of scientists

  20. Stability analysis of a PFTR reactor for a first order kinetic reaction using the Lyapunov functionals

    Directory of Open Access Journals (Sweden)

    Héctor Armando Durán Peralta

    2010-04-01

    Full Text Available The stability of reactors having encompassing concentration and temperature parameters, such as continuous flow stirred tank reactors (CSTR, has been widely explored in the literature; however, there are few papers about the stability of tubular reactor having distributed spatial concentration and temperature parameters such as the plow flow tubular reactor (PFTR. This paper analyses the stability of isothermal and non-isothermal PFTR reactors using the Lyapunov functional method. The first order kinetic reaction was selected because one of this paper’s oblectives was to apply Lyapunov functionals to stability analysis of distributed parameter reactors (technique used in electrical engineering systems’ stability analysis. The stability analysis revealed asymptotically stable tempe- rature and concentration profiles for isothermal PFTR, non-isothermal PFTR with kinetic constant independent of temperature and adiabatic non-isothermal PFTR. Analysis revealed an asymptotically stability region for the heat exchange reactor and an uncertain region where it may have oscillations.

  1. A kinetic sensitivity analysis for the SO2 and NOx removal using the electron beam technology

    Science.gov (United States)

    Zwolińska, Ewa; Gogulancea, Valentina; Sun, Yongxia; Lavric, Vasile; Chmielewski, Andrzej

    2017-09-01

    The mathematical modeling of the phenomena taking place during the electron beam flue gas treatment is a complex endeavor due to the different time scales of the processes occurring as accelerated electrons are bombarding the flue gas. The paper presents a complex kinetic model for these gas phase interactions, consisting of 1034 chemical reactions with the participation of 115 reactive species. The mathematical model couples the complex gas phase kinetics with a liquid phase kinetic model, taking into account the nucleation and condensation phenomena occurring due to the presence of sulfuric acid. The modeling results for both coupled and uncoupled gas phase kinetics are validated against a set of literature experimental data with satisfactory outcome. The work aims to identify the most important chemical reactions influencing the pollutants removal, proposing a sensitivity analysis using the concept of generated entropy. To the best of the authors' knowledge a sensitivity analysis of this extent has not been performed for the electron beam flue gas treatment. The results of this analysis emphasize the link between the removal efficiencies of NOx and SO2, the importance of hydroxyl radicals and can aid in future model reduction efforts.

  2. Development, validation and application of multi-point kinetics model in RELAP5 for analysis of asymmetric nuclear transients

    Energy Technology Data Exchange (ETDEWEB)

    Pradhan, Santosh K., E-mail: santosh@aerb.gov.in [Nuclear Safety Analysis Division, Atomic Energy Regulatory Board, Mumbai 400094 (India); Obaidurrahman, K. [Nuclear Safety Analysis Division, Atomic Energy Regulatory Board, Mumbai 400094 (India); Iyer, Kannan N. [Department of Mechanical Engineering, IIT Bombay, Mumbai 400076 (India); Gaikwad, Avinash J. [Nuclear Safety Analysis Division, Atomic Energy Regulatory Board, Mumbai 400094 (India)

    2016-04-15

    Highlights: • A multi-point kinetics model is developed for RELAP5 system thermal hydraulics code. • Model is validated against extensive 3D kinetics code. • RELAP5 multi-point kinetics formulation is used to investigate critical break for LOCA in PHWR. - Abstract: Point kinetics approach in system code RELAP5 limits its use for many of the reactivity induced transients, which involve asymmetric core behaviour. Development of fully coupled 3D core kinetics code with system thermal-hydraulics is the ultimate requirement in this regard; however coupling and validation of 3D kinetics module with system code is cumbersome and it also requires access to source code. An intermediate approach with multi-point kinetics is appropriate and relatively easy to implement for analysis of several asymmetric transients for large cores. Multi-point kinetics formulation is based on dividing the entire core into several regions and solving ODEs describing kinetics in each region. These regions are interconnected by spatial coupling coefficients which are estimated from diffusion theory approximation. This model offers an advantage that associated ordinary differential equations (ODEs) governing multi-point kinetics formulation can be solved using numerical methods to the desired level of accuracy and thus allows formulation based on user defined control variables, i.e., without disturbing the source code and hence also avoiding associated coupling issues. Euler's method has been used in the present formulation to solve several coupled ODEs internally at each time step. The results have been verified against inbuilt point-kinetics models of RELAP5 and validated against 3D kinetics code TRIKIN. The model was used to identify the critical break in RIH of a typical large PHWR core. The neutronic asymmetry produced in the core due to the system induced transient was effectively handled by the multi-point kinetics model overcoming the limitation of in-built point kinetics model

  3. Performance analysis tool (PATO): Development and preliminary validation

    National Research Council Canada - National Science Library

    Fernando Martins; Filipe Clemente; Frutuoso Silva

    2017-01-01

    .... The Performance Analysis Tool (PATO) software was built with the aim to quickly codify relationships between players and built the adjacency matrices that can be used to test the network measures...

  4. Preliminary Technical Risk Analysis for the Geothermal Technologies Program

    Energy Technology Data Exchange (ETDEWEB)

    McVeigh, J.; Cohen, J.; Vorum, M.; Porro, G.; Nix, G.

    2007-03-01

    This report explains the goals, methods, and results of a probabilistic analysis of technical risk for a portfolio of R&D projects in the DOE Geothermal Technologies Program ('the Program'). The analysis is a task by Princeton Energy Resources International, LLC (PERI), in support of the National Renewable Energy Laboratory (NREL) on behalf of the Program. The main challenge in the analysis lies in translating R&D results to a quantitative reflection of technical risk for a key Program metric: levelized cost of energy (LCOE). This requires both computational development (i.e., creating a spreadsheet-based analysis tool) and a synthesis of judgments by a panel of researchers and experts of the expected results of the Program's R&D.

  5. Integrated transcriptome and methylome analysis in youth at high risk for bipolar disorder: a preliminary analysis.

    Science.gov (United States)

    Fries, G R; Quevedo, J; Zeni, C P; Kazimi, I F; Zunta-Soares, G; Spiker, D E; Bowden, C L; Walss-Bass, C; Soares, J C

    2017-03-14

    First-degree relatives of patients with bipolar disorder (BD), particularly their offspring, have a higher risk of developing BD and other mental illnesses than the general population. However, the biological mechanisms underlying this increased risk are still unknown, particularly because most of the studies so far have been conducted in chronically ill adults and not in unaffected youth at high risk. In this preliminary study we analyzed genome-wide expression and methylation levels in peripheral blood mononuclear cells from children and adolescents from three matched groups: BD patients, unaffected offspring of bipolar parents (high risk) and controls (low risk). By integrating gene expression and DNA methylation and comparing the lists of differentially expressed genes and differentially methylated probes between groups, we were able to identify 43 risk genes that discriminate patients and high-risk youth from controls. Pathway analysis showed an enrichment of the glucocorticoid receptor (GR) pathway with the genes MED1, HSPA1L, GTF2A1 and TAF15, which might underlie the previously reported role of stress response in the risk for BD in vulnerable populations. Cell-based assays indicate a GR hyporesponsiveness in cells from adult BD patients compared to controls and suggest that these GR-related genes can be modulated by DNA methylation, which poses the theoretical possibility of manipulating their expression as a means to counteract the familial risk presented by those subjects. Although preliminary, our results suggest the utility of peripheral measures in the identification of biomarkers of risk in high-risk populations and further emphasize the potential role of stress and DNA methylation in the risk for BD in youth.

  6. Methods for calculating the kinetic parameters of carbonate decomposition from thermal analysis data

    Science.gov (United States)

    Artamonova, I. V.; Gorichev, I. G.; Lainer, Yu. A.; Godunov, E. B.; Kramer, S. M.; Terekhova, M. V.

    2016-07-01

    A procedure is proposed for an analysis of the data on the decomposition of inorganic carbonates (MgCO3, CaCO3, SrCO3, MnCO3, CoCO3) on the basis of results of thermogravimetric studies. The activation energy and the rate constant for carbonate decomposition are calculated taking into account the main points of the heterogeneous reaction kinetics. A comparative analysis of the obtained results is performed.

  7. Noise analysis of ionization kinetics in a protein ion channel

    Science.gov (United States)

    Bezrukov, Sergey M.; Kasianowicz, John J.

    1993-08-01

    We observed excess current noise generated by the reversible ionization of sites in a transmembrane protein ion channel, which is analogous to current fluctuations found recently in solid state microstructure electronic devices. Specifically the current through fully open single channels formed by Staphylococcus aureus α-toxin shows pH dependent fluctuations. We show that noise analysis of the open channel current can be used to evaluate the ionization rate constants, the number of sites participating in the ionization process, and the effect of recharging a single site on the channel conductance.

  8. In situ reaction kinetic analysis of dental restorative materials

    Science.gov (United States)

    Younas, Basma; Samad Khan, Abdul; Muzaffar, Danish; Hussain, Ijaz; Chaudhry, Aqif Anwar; Rehman, Ihtesham Ur

    2013-12-01

    The objective of this study was to evaluate in situ structural and thermal changes of dental restorative materials at periodical time intervals. The commercial materials included zinc oxide eugenol (ZOE), zinc phosphate type I (ZnPO4), glass ionomer cement type II (GIC) and resin-based nano-omposite (Filtek Z350 XT). These materials were processed according to manufacturer's instructions. For the structural analysis Fourier transform infrared spectroscopy (FTIR) was used at high resolution. TGA was used to evaluate thermal weight-loss. The FTIR spectra were collected at periodic time intervals. FTIR spectra showed that with time passing all materials exhibited an increase in peak intensities and a new appearance of shoulders and shifting of peaks for example, ZnPO4 (P-O), ZOE (C═O, C═N, C-O-C), GIC (COO-, C-H, Si-OH), composites (C═O, C═C, C═N, C-N-H). The peaks were replaced by bands and these bands became broader with time interval. Composites showed a degree of conversion and new peaks corresponded to the cross-linking of polymer composites. TGA analysis showed that significant changes in weight loss of set materials were observed after 24 h, where ZOE showed continuous changes in thermal degradation. The spectral changes and thermal degradation with time interval elucidated in situ setting behaviour and understanding of their bonding compatibility with tooth structure and change in relation to time.

  9. Bioelectrical impedance analysis for bovine milk: Preliminary results

    Science.gov (United States)

    Bertemes-Filho, P.; Valicheski, R.; Pereira, R. M.; Paterno, A. S.

    2010-04-01

    This work reports the investigation and analysis of bovine milk quality by using biological impedance measurements using electrical impedance spectroscopy (EIS). The samples were distinguished by a first chemical analysis using Fourier transform midinfrared spectroscopy (FTIR) and flow citometry. A set of milk samples (100ml each) obtained from 17 different cows in lactation with and without mastitis were analyzed with the proposed technique using EIS. The samples were adulterated by adding distilled water and hydrogen peroxide in a controlled manner. FTIR spectroscopy and flow cytometry were performed, and impedance measurements were made in a frequency range from 500Hz up to 1MHz with an implemented EIS system. The system's phase shift was compensated by measuring saline solutions. It was possible to show that the results obtained with the Bioelectrical Impedance Analysis (BIA) technique may detect changes in the milk caused by mastitis and the presence of water and hydrogen peroxide in the bovine milk.

  10. Sensitivity analysis in oxidation ditch modelling: the effect of variations in stoichiometric, kinetic and operating parameters on the performance indices

    NARCIS (Netherlands)

    Abusam, A.A.A.; Keesman, K.J.; Straten, van G.; Spanjers, H.; Meinema, K.

    2001-01-01

    This paper demonstrates the application of the factorial sensitivity analysis methodology in studying the influence of variations in stoichiometric, kinetic and operating parameters on the performance indices of an oxidation ditch simulation model (benchmark). Factorial sensitivity analysis investig

  11. Kinetic analysis on lignite pyrolysis,combustion and gasification

    Institute of Scientific and Technical Information of China (English)

    HU Xin; WANG Zhihua; ZHOU Zhijun; YOU Zhuo; ZHOU Junhu; CEN Kefa

    2013-01-01

    Pyrolysis and combustion combined polygeneration system is a more efficient way for lignite utilization,and can also produce variety of valuable pyrolysis products.To explore the feasibility of this polygeneration system,thermo-gravimetric analysis on YM lignite and DT bituminous coal was conducted,and the characteristic parameters for pyrolysis,combustion,and gasification were obtained.Moreover,the activation energy of each reaction was calculated by Coats-Redfern method.The experimental results showed that,compared with DT bituminous coal,YM lignite pyrolysis process starts at a lower temperature,and its semi-coke reactivity during combustion and gasification was better.In addition,activation energy calculation indicated that,the apparent activation energy of gasification of both coals were much higher than that of the pyrolysis.Therefore,for lignite,pyrolysis and combustion combined polygeneration system may achieve higher efficiency and be worthy for further research.

  12. A Preliminary Analysis of a Behavioral Classrooms Needs Assessment

    Science.gov (United States)

    Leaf, Justin B.; Leaf, Ronald; McCray, Cynthia; Lamkins, Carol; Taubman, Mitchell; McEachin, John; Cihon, Joseph H.

    2016-01-01

    Today many special education classrooms implement procedures based upon the principles of Applied Behavior Analysis (ABA) to establish educationally relevant skills and decrease aberrant behaviors. However, it is difficult for school staff and consultants to evaluate the implementation of various components of ABA and general classroom set up. In…

  13. A Preliminary MANPRINT Evaluation of the All Source Analysis (ASAS)

    Science.gov (United States)

    1988-11-01

    Rear (CEWI) FSIC ............................ 2 CEWI ( TCAE ) AIM(6) ........................... 2 DTOC AIM(6...Sensors and the Ml Battalion TCAE ..... ............... . 13 2. Ratings of Understanding of Tasks Required at the Completion of Training and at the...for transmission to the sensors and jammers. CEWI Tactical Control and Analysis Element ( TCAE ) AIM(6) The AIM module consists of a VAX 750R computer

  14. Anaerobic biodegradation of 13C6-phenol: Analysis of bacteria population involved in two different degradation kinetics

    OpenAIRE

    Hoyos Hernandez, C.; Limam, I.; Desmond, E.; Bouchez, T.; Mazeas, L.

    2013-01-01

    International audience; Biodegradation of 13C6-phenol until mineralisation was observed under mesophilic and thermophilic conditions during the degradation of municipal solid waste. In Mesophilic conditions, as well as in thermophilic, two types of degradation kinetics were obtained. One follows a first order kinetic and the other follows a lag-phase model. The pyrosequencing analysis shows that in the fastest kinetic Clostridium sp. and Anaerobranca sp. were the microorganisms majorly presen...

  15. A practical approach to the sensitivity analysis for kinetic Monte Carlo simulation of heterogeneous catalysis

    CERN Document Server

    Hoffmann, Max J; Matera, Sebastian

    2016-01-01

    Lattice kinetic Monte Carlo simulations have become a vital tool for predictive quality atomistic understanding of complex surface chemical reaction kinetics over a wide range of reaction conditions. In order to expand their practical value in terms of giving guidelines for atomic level design of catalytic systems, it is very desirable to readily evaluate a sensitivity analysis for a given model. The result of such a sensitivity analysis quantitatively expresses the dependency of the turnover frequency, being the main output variable, on the rate constants entering the model. In the past the application of sensitivity analysis, such as Degree of Rate Control, has been hampered by its exuberant computational effort required to accurately sample numerical derivatives of a property that is obtained from a stochastic simulation method. In this study we present an efficient and robust three stage approach that is capable of reliably evaluating the sensitivity measures for stiff microkinetic models as we demonstrat...

  16. Preliminary shielding analysis for the CSNS target station monolith

    Institute of Scientific and Technical Information of China (English)

    张斌; 陈义学; 杨寿海; 吴军; 殷雯; 梁天骄; 贾学军

    2010-01-01

    The construction of the China Spallation Neutron Source (CSNS) has been initiated at Dongguan,Guangdong,China.In spallation neutron sources the target station monolith is contaminated by a large number of fast neutrons whose energies can be as large as those of the protons of the proton beam directed towards the tungsten target.A detailed radiation transport analysis of the target station monolith is important for the construction of the CSNS.The analysis is performed using the coupled Monte Carlo and multi-dimensional discrete ordinates method.Successful elimination of the primary ray effects via the two-dimensional uncollided flux and first collision source methodology is also illustrated.The dose at the edge of the monolith is calculated.The results demonstrate that the doses received by the hall staff members are below the required standard limit.

  17. Preliminary analysis of productivity of fruiting fungi on Strzeleckie meadows

    Directory of Open Access Journals (Sweden)

    Barbara Sadowska

    2014-11-01

    Full Text Available Analysis demonstrated that the fresh ahd dry weight as well as the ash content of fungal fruit bodies collected on a forest-surrounded unmown meadow (Stellario-Deschampsietum Freitag 1957 and Caricetum elatae W.Koch 1926 were lower than the same values for a plot of exploited mown meadow and higher than on an exploited unmown meadow (Arrhenatheretum medioeuropaeum (Br.-Bl. Oberd. 1952.

  18. Preliminary analysis of knee stress in Full Extension Landing

    Directory of Open Access Journals (Sweden)

    Majid Davoodi Makinejad

    2013-09-01

    Full Text Available OBJECTIVE: This study provides an experimental and finite element analysis of knee-joint structure during extended-knee landing based on the extracted impact force, and it numerically identifies the contact pressure, stress distribution and possibility of bone-to-bone contact when a subject lands from a safe height. METHODS: The impact time and loads were measured via inverse dynamic analysis of free landing without knee flexion from three different heights (25, 50 and 75 cm, using five subjects with an average body mass index of 18.8. Three-dimensional data were developed from computed tomography scans and were reprocessed with modeling software before being imported and analyzed by finite element analysis software. The whole leg was considered to be a fixed middle-hinged structure, while impact loads were applied to the femur in an upward direction. RESULTS: Straight landing exerted an enormous amount of pressure on the knee joint as a result of the body's inability to utilize the lower extremity muscles, thereby maximizing the threat of injury when the load exceeds the height-safety threshold. CONCLUSIONS: The researchers conclude that extended-knee landing results in serious deformation of the meniscus and cartilage and increases the risk of bone-to-bone contact and serious knee injury when the load exceeds the threshold safety height. This risk is considerably greater than the risk of injury associated with walking downhill or flexion landing activities.

  19. Preliminary analysis of the mitochondrial genome evolutionary pattern in primates

    Institute of Scientific and Technical Information of China (English)

    Liang ZHAO; Xingtao ZHANG; Xingkui TAO; Weiwei WANG; Ming LI

    2012-01-01

    Since the birth of molecular evolutionary analysis,primates have been a central focus of study and mitochondrial DNA is well suited to these endeavors because of its unique features.Surprisingly,to date no comprehensive evaluation of the nucleotide substitution patterns has been conducted on the mitochondrial genome of primates.Here,we analyzed the evolutionary patterns and evaluated selection and recombination in the mitochondrial genomes of 44 Primates species downloaded from GenBank.The results revealed that a strong rate heterogeneity occurred among sites and genes in all comparisons.Likewise,an obvious decline in primate nucleotide diversity was noted in the subunit rRNAs and tRNAs as compared to the protein-coding genes.Within 13 protein-coding genes,the pattern of nonsynonymous divergence was similar to that of overall nucleotide divergence,while synonymous changes differed only for individual genes,indicating that the rate heterogeneity may result from the rate of change at nonsynonymous sites.Codon usage analysis revealed that there was intermediate codon usage bias in primate protein-coding genes,and supported the idea that GC mutation pressure might determine codon usage and that positive selection is not the driving force for the codon usage bias.Neutrality tests using site-specific positive selection from a Bayesian framework indicated no sites were under positive selection for any gene,consistent with near neutrality.Recombination tests based on the pairwise homoplasy test statistic supported complete linkage even for much older divergent primate species.Thus,with the exception of rate heterogeneity among mitochondrial genes,evaluating the validity assumed complete linkage and selective neutrality in primates prior to phylogenetic or phylogeographic analysis seems unnecessary.

  20. Preliminary analysis of the mitochondrial genome evolutionary pattern in primates.

    Science.gov (United States)

    Zhao, Liang; Zhang, Xingtao; Tao, Xingkui; Wang, Weiwei; Li, Ming

    2012-08-01

    Since the birth of molecular evolutionary analysis, primates have been a central focus of study and mitochondrial DNA is well suited to these endeavors because of its unique features. Surprisingly, to date no comprehensive evaluation of the nucleotide substitution patterns has been conducted on the mitochondrial genome of primates. Here, we analyzed the evolutionary patterns and evaluated selection and recombination in the mitochondrial genomes of 44 Primates species downloaded from GenBank. The results revealed that a strong rate heterogeneity occurred among sites and genes in all comparisons. Likewise, an obvious decline in primate nucleotide diversity was noted in the subunit rRNAs and tRNAs as compared to the protein-coding genes. Within 13 protein-coding genes, the pattern of nonsynonymous divergence was similar to that of overall nucleotide divergence, while synonymous changes differed only for individual genes, indicating that the rate heterogeneity may result from the rate of change at nonsynonymous sites. Codon usage analysis revealed that there was intermediate codon usage bias in primate protein-coding genes, and supported the idea that GC mutation pressure might determine codon usage and that positive selection is not the driving force for the codon usage bias. Neutrality tests using site-specific positive selection from a Bayesian framework indicated no sites were under positive selection for any gene, consistent with near neutrality. Recombination tests based on the pairwise homoplasy test statistic supported complete linkage even for much older divergent primate species. Thus, with the exception of rate heterogeneity among mitochondrial genes, evaluating the validity assumed complete linkage and selective neutrality in primates prior to phylogenetic or phylogeographic analysis seems unnecessary.

  1. Preliminary safety analysis for key design features of KALIMER-600

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Y. B.; Chang, W. P.; Suk, S. D.; Ha, K. S.; Jeong, H. Y.; Heo, S

    2004-03-01

    KAERI is developing the conceptual design of a Liquid Metal Reactor, KALIMER-600 (Korea Advanced LIquid MEtal Reactor) under the Long-term Nuclear R and D Program. KALIMER-600 addresses key issues regarding future nuclear power plants such as plant safety, economics, proliferation, and waste. In this report, key safety design features are described and safety analyses results for typical ATWS accidents in the KALIMER design with breakeven core are presented. First, the basic approach to achieve the safety goal is introduced in Chapter 1, and the event categorization and acceptance criteria for the KALIMER-600 safety analysis are described in Chapter 2. In Chapter 3, results of inherent safety evaluations for the KALIMER-600 conceptual design are presented. The KALIMER-600 core and plant system are designed to assure benign performance during a selected set of events without either reactor control or protection system intervention. Safety analyses for the postulated Anticipated Transient Without Scram (ATWS) have been performed using the SSC-K code to investigate the KALIMER-600 system response to the events. They are categorized as Bounding Events (BEs) because of their low probability of occurrence. In Chapter 4, the analysis of flow blockage for KALIMER-600 with the MATRA-LMR-FB code, which has been developed for the internal flow blockage in a LMR subassembly. The cases with a blockage of 6-subchannel, 24-subchannel, and 54-subchannel are analyzed.The performance analysis of the KALIMER-600 containment and some evaluations for the behaviors during HCDA will be performed later.

  2. Preliminary RAMI analysis of DFLL TBS for ITER

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Dagui [Key Laboratory of Neutronics and Radiation Safety, Institute of Nuclear Energy Safety Technology, Chinese Academy of Sciences, Hefei, Anhui, 230031 (China); University of Science and Technology of China, Hefei, Anhui, 230031 (China); Yuan, Run [Key Laboratory of Neutronics and Radiation Safety, Institute of Nuclear Energy Safety Technology, Chinese Academy of Sciences, Hefei, Anhui, 230031 (China); Wang, Jiaqun, E-mail: jiaqun.wang@fds.org.cn [Key Laboratory of Neutronics and Radiation Safety, Institute of Nuclear Energy Safety Technology, Chinese Academy of Sciences, Hefei, Anhui, 230031 (China); Wang, Fang; Wang, Jin [Key Laboratory of Neutronics and Radiation Safety, Institute of Nuclear Energy Safety Technology, Chinese Academy of Sciences, Hefei, Anhui, 230031 (China)

    2016-11-15

    Highlights: • We performed the functional analysis of the DFLL TBS. • We performed a failure mode analysis of the DFLL TBS. • We estimated the reliability and availability of the DFLL TBS. • The ITER RAMI approach was applied to the DFLL TBS for technical risk control in the design phase. - Abstract: ITER is the first fusion machine fully designed to prove the physics and technological basis for next fusion power plants. Among the main technical objectives of ITER is to test and validate design concepts of tritium breeding blankets relevant to the fusion power plants. To achieve this goal, China has proposed the dual functional lithium-lead test blanket module (DFLL TBM) concept design. The DFLL TBM and its associated ancillary system were called DFLL TBS. The DFLL TBS play a key role in next fusion reactor. In order to ensure reliable and available of DFLL TBS, the risk control project of DFLL TBS has been put on the schedule. As the stage of the ITER technical risk control policy, the RAMI (Reliability, Availability, Maintainability, Inspectability) approach was used to control the technical risk of ITER. In this paper, the RAMI approach was performed on the conceptual design of DFLL TBS. A functional breakdown was prepared on DFLL TBS, and the system was divided into 3 main functions and 72 basic functions. Based on the result of functional breakdown of DFLL TBS, the reliability block diagrams were prepared to estimate the reliability and availability of each function under the stipulated operating conditions. The inherent availability of the DFLL TBS expected after implementation of mitigation actions was calculated to be 98.57% over 2 years based on the ITER reliability database. A Failure Modes Effects and Criticality Analysis (FMECA) was performed with criticality charts highlighting the risk level of the different failure modes with regard to their probability of occurrence and their effects on the availability.

  3. Macroalgae as a Biomass Feedstock: A Preliminary Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Roesijadi, Guritno; Jones, Susanne B.; Snowden-Swan, Lesley J.; Zhu, Yunhua

    2010-09-26

    A thorough of macroalgae analysis as a biofuels feedstock is warranted due to the size of this biomass resource and the need to consider all potential sources of feedstock to meet current biomass production goals. Understanding how to harness this untapped biomass resource will require additional research and development. A detailed assessment of environmental resources, cultivation and harvesting technology, conversion to fuels, connectivity with existing energy supply chains, and the associated economic and life cycle analyses will facilitate evaluation of this potentially important biomass resource.

  4. Statistical Analysis of Time Series Data (STATS). Users Manual (Preliminary)

    Science.gov (United States)

    1987-05-01

    15, 30. 60, 90, 120, andL -!/14:X.... 183 days are presently used. auto Page 1 of 10 wrpy *VtsE0> J1 record (continued) Field Variab Vlue D 2 NPRDS ...each event. 6 JEND + Order number of last period in time series to ( NPRDS ) select for analysis. If blank, the last period is assumed. 7 JPPF Plotting...values. 2 NPRDS + Actual number of periods for the event following on ’INO records until the next ID, BF, or LI record. IN record - T:E SERIES DATA

  5. A Preliminary Genetic Analysis of Complement 3 Gene and Schizophrenia.

    Directory of Open Access Journals (Sweden)

    Jianliang Ni

    Full Text Available Complement pathway activation was found to occur frequently in schizophrenia, and complement 3 (C3 plays a major role in this process. Previous studies have provided evidence for the possible role of C3 in the development of schizophrenia. In this study, we hypothesized that the gene encoding C3 (C3 may confer susceptibility to schizophrenia in Han Chinese. We analyzed 7 common single nucleotide polymorphisms (SNPs of C3 in 647 schizophrenia patients and 687 healthy controls. Peripheral C3 mRNA expression level was measured in 23 drug-naïve patients with schizophrenia and 24 controls. Two SNPs (rs1047286 and rs2250656 that deviated from Hardy-Weinberg equilibrium were excluded for further analysis. Among the remaining 5 SNPs, there was no significant difference in allele and genotype frequencies between the patient and control groups. Logistic regression analysis showed no significant SNP-gender interaction in either dominant model or recessive model. There was no significant difference in the level of peripheral C3 expression between the drug-naïve schizophrenia patients and healthy controls. In conclusion, the results of this study do not support C3 as a major genetic susceptibility factor in schizophrenia. Other factors in AP may have critical roles in schizophrenia and be worthy of further investigation.

  6. Equilibrium and kinetic analysis of human interleukin-13 and IL-13 receptor alpha-2 complex formation.

    Science.gov (United States)

    Lacy, Eilyn R

    2012-03-01

    Interleukin 13 (IL-13) is a pleiotropic cytokine secreted by activated T cells. Both IL-13 and its polymorphic variant (IL-13-R110Q) have been shown to be associated with multiple diseases such as asthma and allergy. Two IL-13 receptors have been identified, IL-13R alpha-1 receptor (IL-13Rα1) and IL-13R alpha-2 receptor (IL-13Rα2). It has been well established that IL-13 binds to IL-13Rα1 alone with low nM affinity while binding to the IL-13Rα1/IL-4R receptor complex is significantly tighter (pM). The affinity between IL-13 and IL-13Rα2, however, remains elusive. Several values have been reported in the literature varying from 20 pM to 2.5 nM. The affinities previously reported were obtained using surface plasmon resonance (SPR) or Scatchard analysis of (125) I-IL-13 binding data. This report presents the results for the kinetics and equilibrium binding analysis studies performed using label-free kinetic exclusion assay (KEA) for the interaction of human IL-13 and IL-13Rα2. KEA equilibrium analysis showed that the affinities of IL-13Rα2 are 107 and 56 pM for IL-13 and its variant (IL-13-R110Q), respectively. KEA kinetic analysis showed that a tight and very stable complex is formed between IL-13Rα2 and IL-13, as shown by calculated dissociation rate constants slower than 5 × 10(-5) per second. Kinetic analysis also showed significant differences in the kinetic behavior of wild type (wt) versus IL-13-R110Q. IL-13-R110Q not only associates to IL-13Rα2 slower than wt human IL-13 (wt-IL-13), as previously reported, but IL-13-R110Q also dissociates slower than wt-IL-13. These results show that IL-13Rα2 is a high affinity receptor and provide a new perspective on kinetic behavior that could have significant implications in the understanding of the role of IL-13-R110Q in the disease state.

  7. Preliminary Rock Physics Analysis on Lodgepole Formation in Manitoba, Canada

    Science.gov (United States)

    Kim, N.; Keehm, Y.

    2012-12-01

    We present rock physics analysis results of Lodgepole Formation, a carbonate reservoir in Daly Field, Manitoba, Canada. We confirmed that the Lodgepole Formation can be divided into six units in the study area: Basal Limestone, Cromer Shale, Cruickshank Crinoidal, Cruickshank Shale, Daly member and Flossie Lake member from the bottom, using eight well log data and previous works. We then performed rock physics analyses on four carbonate units (Basal Limestone, Cruickshank Crinoidal, Daly and Flossie Lake), such as Vp-porosity, AI-porosity, DEM (differential effective medium) modeling, and fluid substitution analysis. In Vp-porosity domain, the top unit, Flossie Lake member has lower porosity and higher velocity, while the other units show similar porosity and velocity. We think that this results from the diagenesis of Flossie Lake member since it bounds with unconformity. However, the four units show very similar trend in Vp-porosity domain, and we can report one Vp-porosity relation for all carbonate units of the Lodgepole formation. We also found that the acoustic impedance varies more than 10% from low porosity zone (3-6%) to high porosity zone (9-12%) from AI-porosity analysis. Thus one can delineate high porosity zone from seismic impedance data. DEM modeling showed that Flossie Lake would have relatively low aspect ratio of pores than the others, which implies that the top unit has been influenced by diagenesis. To determine fluid sensitivity of carbonate units, we conducted fluid substitution on four units from 100% water to 100% oil. The top unit, Flossie Lake, showed slight increase of Vp, which seems to be density effect. The others showed small decrease of Vp, but not significant. If we observe Vp/Vs rather than Vp, the sensitivity increases. However, fluid discrimination would be difficult because of high stiffness of rock frame. In summary, three lower carbonate units of Lodgepole Formation would be prospective and high porosity zone can be delineated

  8. Brain hemisphere dominance and vocational preference: a preliminary analysis.

    Science.gov (United States)

    Szirony, Gary Michael; Pearson, L Carolyn; Burgin, John S; Murray, Gerald C; Elrod, Lisa Marie

    2007-01-01

    Recent developments in split-brain theory add support to the concept of specialization within brain hemispheres. Holland's vocational personality theory may overlap with Human Information Processing (HIP) characteristics. Holland's six RIASEC codes were developed to identify vocational personality characteristics, and HIP scales were designed to measure hemispheric laterality. Relationships between the two scales were evaluated through canonical correlation with some significant results, however not all Holland scale scores correlated with left, right, or integrated hemispheric preference. Additional findings related to participants self-perception of music and math ability were also correlated. Findings on this added analysis revealed a high correlation between perception of musical ability and right brain function but not between mathematical concept and left brain alone. Implications regarding vocational choice and work are discussed.

  9. City of Hoboken Energy Surety Analysis: Preliminary Design Summary

    Energy Technology Data Exchange (ETDEWEB)

    Stamp, Jason Edwin [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Military and Energy Systems Analysis Dept.; Baca, Michael J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Military and Energy Systems Analysis Dept.; Munoz-Ramos, Karina [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Military and Energy Systems Analysis Dept.; Schenkman, Benjamin L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Military and Energy Systems Analysis Dept.; Eddy, John P. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Systems Readiness and Sustainment Technology Dept.; Smith, Mark A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Systems Readiness and Sustainment Technology Dept.; Guttromson, Ross [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Electric Power Systems Research Dept.; Henry, Jordan M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Critical Infrastructure Systems Dept.; Jensen, Richard Pearson [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Geomechanics Dept.

    2014-09-01

    In 2012, Hurricane Sandy devastated much of the U.S. northeast coastal areas. Among those hardest hit was the small community of Hoboken, New Jersey, located on the banks of the Hudson River across from Manhattan. This report describes a city-wide electrical infrastructure design that uses microgrids and other infrastructure to ensure the city retains functionality should such an event occur in the future. The designs ensure that up to 55 critical buildings will retain power during blackout or flooded conditions and include analysis for microgrid architectures, performance parameters, system control, renewable energy integration, and financial opportunities (while grid connected). The results presented here are not binding and are subject to change based on input from the Hoboken stakeholders, the integrator selected to manage and implement the microgrid, or other subject matter experts during the detailed (final) phase of the design effort.

  10. Analysis of organochlorine pesticides in human milk: preliminary results.

    Science.gov (United States)

    Campoy, C; Jiménez, M; Olea-Serrano, M F; Moreno-Frías, M; Cañabate, F; Olea, N; Bayés, R; Molina-Font, J A

    2001-11-01

    In the face of evidence of human milk contamination by organochlorine pesticides, an analysis was performed on samples of milk obtained from healthy lactating women in the provinces of Granada and Almeria in Southern Spain. The samples were obtained by the Neonate Section of the Department of Pediatrics of Granada University Hospital (Neonatology Division) and by the Neonatal Service of Poniente Hospital in El Ejido, Almería. A liquid-liquid extraction procedure was performed. The cleaning of the sample before gas chromatography-mass spectrometry (GC-MS) used silica Sep-Pak. Among other pesticides, aldrin, dieldrin, DDT and its metabolites, lindane, methoxychlor and endosulfan were identified. The presence of these products was confirmed by mass spectrometry. The identification and quantification of these organochlorine molecules is important because they have estrogenic effects.

  11. Preliminary Analysis of a Fully Solid State Magnetocaloric Refrigeration

    Energy Technology Data Exchange (ETDEWEB)

    Abdelaziz, Omar [ORNL

    2016-01-01

    Magnetocaloric refrigeration is an alternative refrigeration technology with significant potential energy savings compared to conventional vapor compression refrigeration technology. Most of the reported active magnetic regenerator (AMR) systems that operate based on the magnetocaloric effect use heat transfer fluid to exchange heat, which results in complicated mechanical subsystems and components such as rotating valves and hydraulic pumps. In this paper, we propose an alternative mechanism for heat transfer between the AMR and the heat source/sink. High-conductivity moving rods/sheets (e.g. copper, brass, iron, graphite, aluminum or composite structures from these) are utilized instead of heat transfer fluid significantly enhancing the heat transfer rate hence cooling/heating capacity. A one-dimensional model is developed to study the solid state AMR. In this model, the heat exchange between the solid-solid interfaces is modeled via a contact conductance, which depends on the interface apparent pressure, material hardness, thermal conductivity, surface roughness, surface slope between the interfaces, and material filled in the gap between the interfaces. Due to the tremendous impact of the heat exchange on the AMR cycle performance, a sensitivity analysis is conducted employing a response surface method, in which the apparent pressure, effective surface roughness and grease thermal conductivity are the uncertainty factors. COP and refrigeration capacity are presented as the response in the sensitivity analysis to reveal the important factors influencing the fully solid state AMR and optimize the solid state AMR efficiency. The performances of fully solid state AMR and traditional AMR are also compared and discussed in present work. The results of this study will provide general guidelines for designing high performance solid state AMR systems.

  12. Preliminary analysis of cerebrospinal fluid proteome in patients with neurocysticercosis

    Institute of Scientific and Technical Information of China (English)

    TIAN Xiao-jun; LI Jing-yi; HUANG Yong; XUE Yan-ping

    2009-01-01

    Background Neurocysticercosis is the infection of the nervous system by the larvae of Taenia solium (T. solium). Despite continuous effort, the experimental diagnosis of neurocysticercosis remains unresolved. Since the cerebrospinal fluid (CSF) contacts with the brain, dynamic information about pathological processes of the brain is likely to be reflected in CSF. Therefore, CSF may serve as a rich source of putative biomarkers related to neurocysticercosis. Comparative proteomic analysis of CSF of neurocysticercosis patients and control subjects may find differentially expressed proteins. Methods Two-dimensional difference in gel electrophoresis (2D-DIGE) was used to investigate differentially expressed proteins in CSF of patients with neurocysticercosis by comparing the protein profile of CSF from neurocysticercosis patients with that from control subjects. The differentially expressed spots/proteins were recognized with matrix-assisted laser desorption/ionization-time of flight-time of flight (MALDI-TOF-TOF) mass spectrometry. Results Forty-four enzyme digested peptides were obtained from 4 neurocysticercotic patients. Twenty-three were identified through search of the NCBI protein database with Mascot software, showing 19 up-expressed and 4 down-expressed. Of these proteins, 26S proteosome related to ATP- and ubiquitin-dependent degradation of proteins and lipocalin type prostaglandin D synthase involved in PGD2-synthesis and extracellular transporter activities were up-expressed, while transferrin related to iron metabolism within the brain was down-expressed. Conclusions This study established the proteomic profile of pooled CSF from 4 patients with neurocysticercosis, suggesting the potential value of proteomic analysis for the study of candidate biomarkers involved in the diagnosis or pathogenesis of neurocysticercosis.

  13. Preliminary Design and Analysis of the GIFTS Instrument Pointing System

    Science.gov (United States)

    Zomkowski, Paul P.

    2003-01-01

    The Geosynchronous Imaging Fourier Transform Spectrometer (GIFTS) Instrument is the next generation spectrometer for remote sensing weather satellites. The GIFTS instrument will be used to perform scans of the Earth s atmosphere by assembling a series of field-of- views (FOV) into a larger pattern. Realization of this process is achieved by step scanning the instrument FOV in a contiguous fashion across any desired portion of the visible Earth. A 2.3 arc second pointing stability, with respect to the scanning instrument, must be maintained for the duration of the FOV scan. A star tracker producing attitude data at 100 Hz rate will be used by the autonomous pointing algorithm to precisely track target FOV s on the surface of the Earth. The main objective is to validate the pointing algorithm in the presence of spacecraft disturbances and determine acceptable disturbance limits from expected noise sources. Proof of concept validation of the pointing system algorithm is carried out with a full system simulation developed using Matlab Simulink. Models for the following components function within the full system simulation: inertial reference unit (IRU), attitude control system (ACS), reaction wheels, star tracker, and mirror controller. With the spacecraft orbital position and attitude maintained to within specified limits the pointing algorithm receives quaternion, ephemeris, and initialization data that are used to construct the required mirror pointing commands at a 100 Hz rate. This comprehensive simulation will also aid in obtaining a thorough understanding of spacecraft disturbances and other sources of pointing system errors. Parameter sensitivity studies and disturbance analysis will be used to obtain limits of operability for the GIFTS instrument. The culmination of this simulation development and analysis will be used to validate the specified performance requirements outlined for this instrument.

  14. Preliminary analysis of the use of smartwatches for longitudinal health monitoring.

    Science.gov (United States)

    Jovanov, Emil

    2015-08-01

    New generations of smartwatches feature continuous measurement of physiological parameters, such as heart rate, galvanic skin resistance (GSR), and temperature. In this paper we present the results of preliminary analysis of the use of Basis Peak smartwatch for longitudinal health monitoring during a 4 month period. Physiological measurements during sleep are validated using Zephyr Bioharness 3 monitor and SOMNOscreen+ polysomnographic monitoring system from SOMNOmedics. Average duration of sequences with no missed data was 49.9 minutes, with maximum length of 17 hours, and they represent 88.88% of recording time. Average duration of the charging event was 221.9 min, and average time between charges was 54 hours, with maximum duration of the charging event of 16.3 hours. Preliminary results indicate that the physiological monitoring performance of existing smartwatches provides sufficient performance for longitudinal monitoring of health status and analysis of health and wellness trends.

  15. Relative risk analysis in regulating the use of radiation-emitting medical devices. A preliminary application

    Energy Technology Data Exchange (ETDEWEB)

    Jones, E.D.; Banks, W.W.; Altenbach, T.J.; Fischer, L.E. [Lawrence Livermore National Lab., CA (United States)

    1995-09-01

    This report describes a preliminary application of an analysis approach for assessing relative risks in the use of radiation- emitting medical devices. Results are presented on human-initiated actions and failure modes that are most likely to occur in the use of the Gamma Knife, a gamma irradiation therapy device. This effort represents an initial step in a US Nuclear Regulatory Commission (NRC) plan to evaluate the potential role of risk analysis in regulating the use of nuclear medical devices. For this preliminary application of risk assessment, the focus was to develop a basic process using existing techniques for identifying the most likely risk contributors and their relative importance. The approach taken developed relative risk rankings and profiles that incorporated the type and quality of data available and could present results in an easily understood form. This work was performed by the Lawrence Livermore National Laboratory for the NRC.

  16. Analysis of Equilibrium and Kinetics of Chromium-Fluoride Complexation from Spectroscopic Data via Chemometrics Methods

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The complexation of Cr3+ with F- undergoes a multistep reversible process. An approach to research the involved equilibria and kinetics using suitable chemometrics methods to the online measured UV-Vis spectra is proposed. By investigating the equilibrium spectra of the complexes at different molar ratios of M to L(metal to ligand) and 50 ℃, the result of Principal Component Analysis(PCA) shows that three complexes, ML, ML2 and ML3, can be formed under the research conditions. The spectrum of each complex was then analyzed and the accumulated equilibrium constants were calculated by applying Target Testing Factor Analysis(TTFA). Meanwhile, a reactive intermediate was observed before the formation of MLx during the specific kinetic study at 15 ℃. The equilibrium constant and spectrum of the intermediate as well as the rate constants were all resolved by using TTFA.

  17. Quantitative Förster resonance energy transfer analysis for kinetic determinations of SUMO-specific protease.

    Science.gov (United States)

    Liu, Yan; Song, Yang; Madahar, Vipul; Liao, Jiayu

    2012-03-01

    Förster resonance energy transfer (FRET) technology has been widely used in biological and biomedical research, and it is a very powerful tool for elucidating protein interactions in either dynamic or steady state. SUMOylation (the process of SUMO [small ubiquitin-like modifier] conjugation to substrates) is an important posttranslational protein modification with critical roles in multiple biological processes. Conjugating SUMO to substrates requires an enzymatic cascade. Sentrin/SUMO-specific proteases (SENPs) act as an endopeptidase to process the pre-SUMO or as an isopeptidase to deconjugate SUMO from its substrate. To fully understand the roles of SENPs in the SUMOylation cycle, it is critical to understand their kinetics. Here, we report a novel development of a quantitative FRET-based protease assay for SENP1 kinetic parameter determination. The assay is based on the quantitative analysis of the FRET signal from the total fluorescent signal at acceptor emission wavelength, which consists of three components: donor (CyPet-SUMO1) emission, acceptor (YPet) emission, and FRET signal during the digestion process. Subsequently, we developed novel theoretical and experimental procedures to determine the kinetic parameters, k(cat), K(M), and catalytic efficiency (k(cat)/K(M)) of catalytic domain SENP1 toward pre-SUMO1. Importantly, the general principles of this quantitative FRET-based protease kinetic determination can be applied to other proteases. Copyright © 2011 Elsevier Inc. All rights reserved.

  18. Quantification of DNA repair protein kinetics after γ-irradiation using number and brightness analysis

    Science.gov (United States)

    Abdisalaam, Salim; Poudel, Milan; Chen, David J.; Alexandrakis, George

    2011-03-01

    The kinetics of most proteins involved in DNA damage sensing, signaling and repair following ionizing radiation exposure cannot be quantified by current live cell fluorescence microscopy methods. This is because most of these proteins, with only few notable exceptions, do not attach in large numbers at DNA damage sites to form easily detectable foci in microscopy images. As a result a high fluorescence background from freely moving and immobile fluorescent proteins in the nucleus masks the aggregation of proteins at sparse DNA damage sites. Currently, the kinetics of these repair proteins are studied by laser-induced damage and Fluorescence Recovery After Photobleaching that rely on the detectability of high fluorescence intensity spots of clustered DNA damage. We report on the use of Number and Brightness (N&B) analysis methods as a means to monitor kinetics of DNA repair proteins during sparse DNA damage created by γ-irradiation, which is more relevant to cancer treatment than laser-induced clustered damage. We use two key double strand break repair proteins, namely Ku 70/80 and the DNA-dependent protein kinase catalytic subunit (DNA-PKCS), as specific examples to showcase the feasibility of the proposed methods to quantify dose-dependent kinetics for DNA repair proteins after exposure to γ-rays.

  19. Raman mapping for kinetic analysis of crystallization of amorphous drug based on distributional images.

    Science.gov (United States)

    Ueda, Hiroshi; Ida, Yasuo; Kadota, Kazunori; Tozuka, Yuichi

    2014-02-28

    The feasibility of Raman mapping for understanding the crystallization mechanism of an amorphous drug was investigated using described images. The crystallization tendency of amorphous indomethacin under dry condition at 30 °C was kinetically evaluated by means of Raman mapping and X-ray powder diffraction (XRPD) with change in the calculated crystallinities. Raman images directly revealed the occurrence of particle size-dependent non-uniform crystallization; slow crystallization of large particles, but fast crystallization of small particles. Kinetic analysis by fitting to the Kolmogorov-Johnson-Mehl-Avrami equation was performed for the crystallization profiles of both Raman mapping and XRPD data. For the Raman mapping data, the distribution of large particles was characterized and examined. The kinetic parameters calculated from the whole Raman image area agreed well with those of XRPD, suggesting accurate prediction of both techniques for the entire crystallization. Raman images revealed the change in the crystallization mechanism for the focused area; the large particles showed a reduced crystallization rate constant and an increase in the dimensional crystal growth exponent. Raman mapping is an attractive tool for quantitative and kinetic investigation of the crystallization mechanism with distributional images.

  20. Thermogravimetric analysis and kinetic study on pyrolysis of representative medical waste composition.

    Science.gov (United States)

    Deng, Na; Zhang, Yu-feng; Wang, Yan

    2008-01-01

    To obtain detailed information on the pyrolysis characteristics, a thermogravimetric study on the pyrolysis of 14 typical medical waste compositions was carried out in thermogravimetric analysis (TGA) equipment using dynamic techniques in a stream of N2. An index representing pyrolysis reactivity of waste was presented. Kinetic parameters were obtained by Coats-Redfern method and used to model the TG curve. The results showed that: (a) Plastic, protein, cellulosic material, synthetic fibre, and rubber entered pyrolysis process in succession. (b) There was one decomposition stage in the pyrolysis of one-off medical glove, operating glove, cellulosic waste, absorbable catgut suture and adhesive plaster, while other components had two obvious weight loss stages. (c) The obtained apparent activation energy for second stage pyrolysis was comparably higher than that for first stage. (d) Each stage was controlled by only one kinetic mechanism, in which kinetic parameters were constant. (e) The degradation kinetics of medical waste may be affected by special physical and chemical treatment in the product manufacturing process. (f) Among 13 waste samples, the pyrolysis index of cellulosic matter was the highest, which indicated cellulosic matter had strong pyrolysis reactivity. (g) With increasing heating rate, TG curve and DTG peak shifted to high temperatures and main reaction interval of the sample became longer.

  1. On-Line Analysis and Kinetic Behavior of Arsenic Release during Coal Combustion and Pyrolysis.

    Science.gov (United States)

    Shen, Fenghua; Liu, Jing; Zhang, Zhen; Dai, Jinxin

    2015-11-17

    The kinetic behavior of arsenic (As) release during coal combustion and pyrolysis in a fluidized bed was investigated by applying an on-line analysis system of trace elements in flue gas. This system, based on inductively coupled plasma optical emission spectroscopy (ICP-OES), was developed to measure trace elements concentrations in flue gas quantitatively and continuously. Obvious variations of arsenic concentration in flue gas were observed during coal combustion and pyrolysis, indicating strong influences of atmosphere and temperature on arsenic release behavior. Kinetic laws governing the arsenic release during coal combustion and pyrolysis were determined based on the results of instantaneous arsenic concentration in flue gas. A second-order kinetic law was determined for arsenic release during coal combustion, and the arsenic release during coal pyrolysis followed a fourth-order kinetic law. The results showed that the arsenic release rate during coal pyrolysis was faster than that during coal combustion. Thermodynamic calculations were carried out to identify the forms of arsenic in vapor and solid phases during coal combustion and pyrolysis, respectively. Ca3(AsO4)2 and Ca(AsO2)2 are the possible species resulting from As-Ca interaction during coal combustion. Ca(AsO2)2 is the most probable species during coal pyrolysis.

  2. A Preliminary Tsunami Vulnerability Analysis for Yenikapi Region in Istanbul

    Science.gov (United States)

    Ceren Cankaya, Zeynep; Suzen, Lutfi; Cevdet Yalciner, Ahmet; Kolat, Cagil; Aytore, Betul; Zaytsev, Andrey

    2015-04-01

    One of the main requirements during post disaster recovery operations is to maintain proper transportation and fluent communication at the disaster areas. Ports and harbors are the main transportation hubs which must work with proper performance at all times especially after the disasters. Resilience of coastal utilities after earthquakes and tsunamis have major importance for efficient and proper rescue and recovery operations soon after the disasters. Istanbul is a mega city with its various coastal utilities located at the north coast of the Sea of Marmara. At Yenikapi region of Istanbul, there are critical coastal utilities and vulnerable coastal structures and critical activities occur daily. Fishery ports, commercial ports, small craft harbors, passenger terminals of intercity maritime transportation, water front commercial and/or recreational structures are some of the examples of coastal utilization which are vulnerable against marine disasters. Therefore their vulnerability under tsunami or any other marine hazard to Yenikapi region of Istanbul is an important issue. In this study, a methodology of vulnerability analysis under tsunami attack is proposed with the applications to Yenikapi region. In the study, high resolution (1m) GIS database of Istanbul Metropolitan Municipality (IMM) is used and analyzed by using GIS implementation. The bathymetry and topography database and the vector dataset containing all buildings/structures/infrastructures in the study area are obtained for tsunami numerical modeling for the study area. GIS based tsunami vulnerability assessment is conducted by applying the Multi-criteria Decision Making Analysis (MCDA). The tsunami parameters from deterministically defined worst case scenarios are computed from the simulations using tsunami numerical model NAMI DANCE. The vulnerability parameters in the region due to two different classifications i) vulnerability of buildings/structures and ii) vulnerability of (human) evacuation

  3. Sammon mapping for preliminary analysis in Hyperspectral Imagery

    Directory of Open Access Journals (Sweden)

    Nicolae APOSTOLESCU

    2016-03-01

    Full Text Available The main goal of this paper is to present the implementation of the Sammon algorithm developed for finding N points in a lower m-dimensional subspace, where the original points are from a high n-dimensional space. This mapping is done so interpoints Euclidian distances in m-space correspond to the distances measured in the n-dimensional space. This method known as non-linear projection method or multidimensional scaling (MDS aims to preserve the global properties of points. The method is based on the idea of transforming the original, n-dimensional input space into a reduced, m-dimensional one, where mAnalysis (PCA may be applied as a pre-processing procedure for starting, in order to obtain the N points in the lower subspace. The algorithm was tested on hyperspectral data with spectra of various lengths. Depending of the size of the input data (number of points, the number of learning iterations and computational facilities available, Sammon mapping might be computationally expensive.

  4. A simplified procedure of linear regression in a preliminary analysis

    Directory of Open Access Journals (Sweden)

    Silvia Facchinetti

    2013-05-01

    Full Text Available The analysis of a statistical large data-set can be led by the study of a particularly interesting variable Y – regressed – and an explicative variable X, chosen among the remained variables, conjointly observed. The study gives a simplified procedure to obtain the functional link of the variables y=y(x by a partition of the data-set into m subsets, in which the observations are synthesized by location indices (mean or median of X and Y. Polynomial models for y(x of order r are considered to verify the characteristics of the given procedure, in particular we assume r= 1 and 2. The distributions of the parameter estimators are obtained by simulation, when the fitting is done for m= r + 1. Comparisons of the results, in terms of distribution and efficiency, are made with the results obtained by the ordinary least square methods. The study also gives some considerations on the consistency of the estimated parameters obtained by the given procedure.

  5. Preliminary Analysis of Slope Stability in Kuok and Surrounding Areas

    Directory of Open Access Journals (Sweden)

    Dewandra Bagus Eka Putra

    2016-12-01

    Full Text Available The level of slope influenced by the condition of the rocks beneath the surface. On high level of slopes, amount of surface runoff and water transport energy is also enlarged. This caused by greater gravity, in line with the surface tilt from the horizontal plane. In other words, topsoil eroded more and more. When the slope becomes twice as steep, then the amount of erosion per unit area be 2.0 - 2.5 times more. Kuok and surrounding area is the road access between the West Sumatra and Riau which plays an important role economies of both provinces. The purpose of this study is to map the locations that have fairly steep slopes and potential mode of landslides. Based on SRTM data obtained,  the roads in Kuok area has a minimum elevation of + 33 m and a maximum  + 217.329 m. Rugged road conditions with slope ranging from 24.08 ° to 44.68 ° causing this area having frequent landslides. The result of slope stability analysis in a slope near the Water Power Plant Koto Panjang, indicated that mode of active failure is toppling failure or rock fall and the potential zone of failure is in the center part of the slope.

  6. Social network analysis in identifying influential webloggers: A preliminary study

    Science.gov (United States)

    Hasmuni, Noraini; Sulaiman, Nor Intan Saniah; Zaibidi, Nerda Zura

    2014-12-01

    In recent years, second generation of internet-based services such as weblog has become an effective communication tool to publish information on the Web. Weblogs have unique characteristics that deserve users' attention. Some of webloggers have seen weblogs as appropriate medium to initiate and expand business. These webloggers or also known as direct profit-oriented webloggers (DPOWs) communicate and share knowledge with each other through social interaction. However, survivability is the main issue among DPOW. Frequent communication with influential webloggers is one of the way to keep survive as DPOW. This paper aims to understand the network structure and identify influential webloggers within the network. Proper understanding of the network structure can assist us in knowing how the information is exchanged among members and enhance survivability among DPOW. 30 DPOW were involved in this study. Degree centrality and betweenness centrality measurement in Social Network Analysis (SNA) were used to examine the strength relation and identify influential webloggers within the network. Thus, webloggers with the highest value of these measurements are considered as the most influential webloggers in the network.

  7. SLUDGE TREATMENT PROJECT ENGINEERED CONTAINER RETRIEVAL AND TRANSFER SYSTEM PRELIMINARY DESIGN HAZARD ANALYSIS SUPPLEMENT 1

    Energy Technology Data Exchange (ETDEWEB)

    FRANZ GR; MEICHLE RH

    2011-07-18

    This 'What/If' Hazards Analysis addresses hazards affecting the Sludge Treatment Project Engineered Container Retrieval and Transfer System (ECRTS) NPH and external events at the preliminary design stage. In addition, the hazards of the operation sequence steps for the mechanical handling operations in preparation of Sludge Transport and Storage Container (STSC), disconnect STSC and prepare STSC and Sludge Transport System (STS) for shipping are addressed.

  8. Preliminary Failure Modes and Effects Analysis of the US Massive Gas Injection Disruption Mitigation System Design

    Energy Technology Data Exchange (ETDEWEB)

    Lee C. Cadwallader

    2013-10-01

    This report presents the results of a preliminary failure modes and effects analysis (FMEA) of a candidate design for the ITER Disruption Mitigation System. This candidate is the Massive Gas Injection System that provides machine protection in a plasma disruption event. The FMEA was quantified with “generic” component failure rate data as well as some data calculated from operating facilities, and the failure events were ranked for their criticality to system operation.

  9. Preliminary Failure Modes and Effects Analysis of the US DCLL Test Blanket Module

    Energy Technology Data Exchange (ETDEWEB)

    Lee C. Cadwallader

    2007-08-01

    This report presents the results of a preliminary failure modes and effects analysis (FMEA) of a small tritium-breeding test blanket module design for the International Thermonuclear Experimental Reactor. The FMEA was quantified with “generic” component failure rate data, and the failure events are binned into postulated initiating event families and frequency categories for safety assessment. An appendix to this report contains repair time data to support an occupational radiation exposure assessment for test blanket module maintenance.

  10. Preliminary Failure Modes and Effects Analysis of the US DCLL Test Blanket Module

    Energy Technology Data Exchange (ETDEWEB)

    Lee C. Cadwallader

    2010-06-01

    This report presents the results of a preliminary failure modes and effects analysis (FMEA) of a small tritium-breeding test blanket module design for the International Thermonuclear Experimental Reactor. The FMEA was quantified with “generic” component failure rate data, and the failure events are binned into postulated initiating event families and frequency categories for safety assessment. An appendix to this report contains repair time data to support an occupational radiation exposure assessment for test blanket module maintenance.

  11. Unscented Kalman filter with parameter identifiability analysis for the estimation of multiple parameters in kinetic models

    OpenAIRE

    Baker Syed; Poskar C; Junker Björn

    2011-01-01

    Abstract In systems biology, experimentally measured parameters are not always available, necessitating the use of computationally based parameter estimation. In order to rely on estimated parameters, it is critical to first determine which parameters can be estimated for a given model and measurement set. This is done with parameter identifiability analysis. A kinetic model of the sucrose accumulation in the sugar cane culm tissue developed by Rohwer et al. was taken as a test case model. Wh...

  12. KINETIC MODELLING AND PERFORMANCE ANALYSIS OF THE FOUR-POST-FRAME LIFTINGMECHANICAL SYSTEM

    Institute of Scientific and Technical Information of China (English)

    高建华; 杨汝清; 胡洪国

    2001-01-01

    The kinetic model of the four-post-frame lifting mechanical system was established. The stiffness and damping matrices of differential equations of motion were obtained by using Lagrange's equations. And the dynamic characteristics of system were analyzed by modal analysis method. Based upon this, the modifications of structural parameters which can improve dynamic performance were discussed. The low-level high-speed palletizer MDJ1200L was taken as a real case in the paper.

  13. Analysis for the Sorption Kinetics of Ag Nanoparticles on Natural Clinoptilolite

    OpenAIRE

    Alvaro Ruíz-Baltazar; Simón Yobanny Reyes-López; Oswald Tellez-Vasquez; Rodrigo Esparza; Gerardo Rosas; Ramiro Pérez

    2015-01-01

    The kinetic adsorption behavior of silver nanoparticles deposited on a natural zeolite from Oaxaca is presented. Theoretical models as Lagergren first-order, pseudo-second-order, Elovich, and intraparticle diffusion were employed and compared with experimental data obtained by atomic absorption spectrophotometry technique. Correlation factors R2 of the order of 0.99 were observed. Analysis by transmission electron microscopy revealed that the silver nanoparticles were homogeneously distribute...

  14. PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ACUTE ORAL TOXICITY STUDY OF CLITORIA TERNATEA LINN. ROOTS IN ALBINO MICE

    Directory of Open Access Journals (Sweden)

    Deka Manalisha

    2011-12-01

    Full Text Available Clitoria ternatea has been using since the ancient times for its medicinal values. Almost all the parts of the plant have medicinal property. The root of the plant is reported to have anti diarrheal, Anti histamic, cholinergic activity etc. Traditionally the root has been using for the treatment of many diseases like leucorrhoea, diarrhea, urinary problems, diuretic, impotency, stomach trouble etc. The present study was designed to investigate the preliminary phytochemical analysis and acute oral toxicity of the root of the plant. The shed dried materials were grinded and used in the study. The preliminary phytochemical analysis was done by following standard protocols. For acute oral toxicity study, methanolic extract of the root was used. The extract was prepared by standard protocol. The preliminary phytochemical analysis showed the presence of proteins, carbohydrates, glycosides, resins, saponin, flavonoid, alkaloids, steroids and phenol. The acute oral toxicity study showed no mortality up to a dose of 3000 mg per kg body weight. The presence of plant chemicals revealed the medicinal values and the non toxic property of the plant indicated the value of the plant as medicine. Thus we can conclude that, the root of the plant can be used as a safe drug against many diseases.

  15. PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ACUTE ORAL TOXICITY STUDY OF MUCUNA PRURIENS LINN. IN ALBINO MICE

    Directory of Open Access Journals (Sweden)

    Deka Manalisha

    2012-02-01

    Full Text Available Mucuna Pruriens Linn. is an annual, climbing shrub which has an important place among aphrodisiac herbs in India since the ancient times. The plant has been using traditionally for many medicinal purposes such as Infertility, Parkinson’s disease, Loss of libido, Antioxidant, Anti venom, Anti microbial etc. The present study was carried out to investigate the preliminary phytochemical analysis and acute oral toxicity of the seeds of M.pruriens on albino mice. Matured seeds of M.pruriens were dried in shed and grinded in a mechanical grinder. The preliminary phytochemical analysis was done by following standard protocols. For acute oral toxicity study, methanolic extract of the seeds were used. The extract was prepared in a Soxlet apparatus. The preliminary phytochemical analysis showed the presence of protein, carbohydrates, glycosides, alkaloids, steroids, flavonoids, phenols and tannins. The acute oral toxicity study showed no mortality up to a dose of 4000 mg per kg body weight. The presence of plant chemicals revealed the medicinal values and the non toxic property of the plant indicated the value of the plant as medicine. Thus, we can conclude that, the seed of the plant can be used as a safe drug against many diseases.

  16. Branched pore kinetic model analysis of geosmin adsorption on super-powdered activated carbon.

    Science.gov (United States)

    Matsui, Yoshihiko; Ando, Naoya; Sasaki, Hiroshi; Matsushita, Taku; Ohno, Koichi

    2009-07-01

    Super-powdered activated carbon (S-PAC) is activated carbon of much finer particle size than powdered activated carbon (PAC). Geosmin is a naturally occurring taste and odor compound that impairs aesthetic quality in drinking water. Experiments on geosmin adsorption on S-PAC and PAC were conducted, and the results using adsorption kinetic models were analyzed. PAC pulverization, which produced the S-PAC, did not change geosmin adsorption capacity, and geosmin adsorption capacities did not differ between S-PAC and PAC. Geosmin adsorption kinetics, however, were much higher on S-PAC than on PAC. A solution to the branched pore kinetic model (BPKM) was developed, and experimental adsorption kinetic data were analyzed by BPKM and by a homogeneous surface diffusion model (HSDM). The HSDM describing the adsorption behavior of geosmin required different surface diffusivity values for S-PAC and PAC, which indicated a decrease in surface diffusivity apparently associated with activated carbon particle size. The BPKM, consisting of macropore diffusion followed by mass transfer from macropore to micropore, successfully described the batch adsorption kinetics on S-PAC and PAC with the same set of model parameter values, including surface diffusivity. The BPKM simulation clearly showed geosmin removal was improved as activated carbon particle size decreased. The simulation also implied that the rate-determining step in overall mass transfer shifted from intraparticle radial diffusion in macropores to local mass transfer from macropore to micropore. Sensitivity analysis showed that adsorptive removal of geosmin improved with decrease in activated carbon particle size down to 1microm, but further particle size reduction produced little improvement.

  17. Thermogravimetric Analysis and Kinetic Study on Pyrolysis of Veteri-narian Solid Waste

    Directory of Open Access Journals (Sweden)

    Andrés Felipe Rojas González

    2016-10-01

    Full Text Available Context: Institutional waste from clinical centers can be classified as those coming from health institutions dedicated to human attention and those coming from centers for animal veterinary care. The latter are mainly hazardous wastes, hence their disposal requires incineration. Most of such waste is organic, and it is possible, therefore, to take advantage of their energetic power in combustion or pyrolysis processes. This work is motivated because no literature was found on the pyrolysis kinetics veterinary waste, as this kind of studies are mainly focused on hospital waste of human health care. Method: The kinetics of pyrolysis is characterized and studied by means of thermogravimetric analysis of 6 major veterinary waste (gauze, cotton swabs, cotton, nails, hair, plastic syringes. The characterization is performed by proximate and elemental analysis, and thermogravimetric analysis. Reactivity characteristics and pyrolytic capability of wastes are established. The kinetics study on pyrolysis was carried out by determining the kinetic triplet by isoconversional Starink method. Results: It was established that the pyrolysis index increases with the heating rate and that the thermal degradation depends on the material type of the waste. Similarly, it was found that the temperature (ΔT = Tf - Ti for the thermal decomposition of veterinary waste is: ΔTnails> ΔThair > ΔTcotton swabs > ΔTgauze > ΔTcotton > ΔTplastic syringes; the activation energy is Enails> E hair > Eplastic syringes > Ecotton swabs > E gauze > Ecotton, and the reaction order is: n hair > nnails > ncotton swabs > ncotton > n gauze > n plastic syringes. Conclusions: These results suggest the possibility of using veterinary wastes for power generation, providing an alternative for sustainable energy development to cities in continuous growth, from both, energetic and environmental points of view.

  18. Kinetic Analysis of the Digestion of Bovine Type I Collagen Telopeptides with Porcine Pepsin.

    Science.gov (United States)

    Qian, Jun; Okada, Yukari; Ogura, Takayuki; Tanaka, Keisuke; Hattori, Shunji; Ito, Shinji; Satoh, Junko; Takita, Teisuke; Yasukawa, Kiyoshi

    2016-01-01

    Collagen is frequently digested using pepsin in industries to produce a triple helical collagen without the N- and C-terminal telopeptides. However, kinetic analysis of this reaction is difficult because several Lys residues in the N- and in the C-terminal telopeptides form covalent bonds, leading to multiple substrates species, and pepsin cleaves collagen at various sites in the N-terminal and in the C-terminal telopeptides, yielding different products. Here we performed kinetic analysis of the digestion of bovine type I collagen with porcine pepsin. The reaction could be monitored by SDS-PAGE by measuring the intensity of the protein bands corresponding to the variant β11 chain. We obtained kinetic parameters relative to the decrease in the variant β11 chain upon digestion. At pH 4.0, the Km and kcat values increased with increasing temperature (30 to 65 °C), although the kcat /Km values were stable. Additional cleavage at the helical region was detected at 45 to 65 °C. At 37 °C, the Km and kcat values increased with decreasing pH, and the kcat /Km values at pH 2.1 to 4.5 were stable and higher than those at pH 5.0 and 5.5. No additional cleavage was detected at the examined pH. Thus, the optimal pH and temperatures for selective digestion of collagen telopeptides with pepsin are 2.1 to 4.5 and 30 to 40 °C, respectively. These results suggest that the method might be useful for the kinetic analysis of the digestion of collagen telopeptides with pepsin.

  19. Preliminary CFD Analysis for HVAC System Design of a Containment Building

    Energy Technology Data Exchange (ETDEWEB)

    Son, Sung Man; Choi, Choengryul [ELSOLTEC, Yongin (Korea, Republic of); Choo, Jae Ho; Hong, Moonpyo; Kim, Hyungseok [KEPCO Engineering and Construction, Gimcheon (Korea, Republic of)

    2016-10-15

    HVAC (Heating, Ventilation, Air Conditioning) system has been mainly designed based on overall heat balance and averaging concepts, which is simple and useful for designing overall system. However, such a method has the disadvantage that cannot predict the local flow and temperature distributions in a containment building. In this study, a CFD (Computational Fluid Dynamics) preliminary analysis is carried out to obtain detailed flow and temperature distributions in a containment building and to ensure that such information can be obtained via CFD analysis. This approach can be useful for hydrogen analysis in an accident related to hydrogen released into a containment building. In this study, CFD preliminary analysis has been performed to obtain the detailed information of the reactor containment building by using the CFD analysis techniques and to ensure that such information can be obtained via CFD analysis. We confirmed that CFD analysis can offer enough detailed information about flow patterns and temperature field and that CFD technique is a useful tool for HVAC design of nuclear power plants.

  20. Mechanistic analysis of multi-omics datasets to generate kinetic parameters for constraint-based metabolic models

    Directory of Open Access Journals (Sweden)

    Cotten Cameron

    2013-01-01

    Full Text Available Abstract Background Constraint-based modeling uses mass balances, flux capacity, and reaction directionality constraints to predict fluxes through metabolism. Although transcriptional regulation and thermodynamic constraints have been integrated into constraint-based modeling, kinetic rate laws have not been extensively used. Results In this study, an in vivo kinetic parameter estimation problem was formulated and solved using multi-omic data sets for Escherichia coli. To narrow the confidence intervals for kinetic parameters, a series of kinetic model simplifications were made, resulting in fewer kinetic parameters than the full kinetic model. These new parameter values are able to account for flux and concentration data from 20 different experimental conditions used in our training dataset. Concentration estimates from the simplified kinetic model were within one standard deviation for 92.7% of the 790 experimental measurements in the training set. Gibbs free energy changes of reaction were calculated to identify reactions that were often operating close to or far from equilibrium. In addition, enzymes whose activities were positively or negatively influenced by metabolite concentrations were also identified. The kinetic model was then used to calculate the maximum and minimum possible flux values for individual reactions from independent metabolite and enzyme concentration data that were not used to estimate parameter values. Incorporating these kinetically-derived flux limits into the constraint-based metabolic model improved predictions for uptake and secretion rates and intracellular fluxes in constraint-based models of central metabolism. Conclusions This study has produced a method for in vivo kinetic parameter estimation and identified strategies and outcomes of kinetic model simplification. We also have illustrated how kinetic constraints can be used to improve constraint-based model predictions for intracellular fluxes and biomass

  1. Mechanistic analysis of multi-omics datasets to generate kinetic parameters for constraint-based metabolic models.

    Science.gov (United States)

    Cotten, Cameron; Reed, Jennifer L

    2013-01-30

    Constraint-based modeling uses mass balances, flux capacity, and reaction directionality constraints to predict fluxes through metabolism. Although transcriptional regulation and thermodynamic constraints have been integrated into constraint-based modeling, kinetic rate laws have not been extensively used. In this study, an in vivo kinetic parameter estimation problem was formulated and solved using multi-omic data sets for Escherichia coli. To narrow the confidence intervals for kinetic parameters, a series of kinetic model simplifications were made, resulting in fewer kinetic parameters than the full kinetic model. These new parameter values are able to account for flux and concentration data from 20 different experimental conditions used in our training dataset. Concentration estimates from the simplified kinetic model were within one standard deviation for 92.7% of the 790 experimental measurements in the training set. Gibbs free energy changes of reaction were calculated to identify reactions that were often operating close to or far from equilibrium. In addition, enzymes whose activities were positively or negatively influenced by metabolite concentrations were also identified. The kinetic model was then used to calculate the maximum and minimum possible flux values for individual reactions from independent metabolite and enzyme concentration data that were not used to estimate parameter values. Incorporating these kinetically-derived flux limits into the constraint-based metabolic model improved predictions for uptake and secretion rates and intracellular fluxes in constraint-based models of central metabolism. This study has produced a method for in vivo kinetic parameter estimation and identified strategies and outcomes of kinetic model simplification. We also have illustrated how kinetic constraints can be used to improve constraint-based model predictions for intracellular fluxes and biomass yield and identify potential metabolic limitations through the

  2. GC-MS analysis, preliminary phytochemical screening, physicochemical analysis and anti-diabetic activity of ethanol extract of Jasminum cuspidatum leaves

    National Research Council Canada - National Science Library

    Singumsetty Vinay; Shaik Karimulla; Devarajan Saravanan

    2014-01-01

    The purpose of the present study was investigating the GC-MS analysis, preliminary phytochemical screening, physicochemical analysis and anti-diabetic activity of ethanol extract of the leaves of Jasminum cuspidatum...

  3. ANSI/ASHRAE/IESNA Standard 90.1-2010 Preliminary Determination Quantitative Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Halverson, Mark A.; Liu, Bing; Rosenberg, Michael I.

    2010-11-01

    The United States (U.S.) Department of Energy (DOE) conducted a preliminary quantitative analysis to assess whether buildings constructed according to the requirements of the American National Standards Institute (ANSI)/American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE)/Illuminating Engineering Society of North America (IESNA) Standard 90.1-2010 (ASHRAE Standard 90.1-2010, Standard 90.1-2010, or 2010 edition) would result in energy savings compared with buildings constructed to ANSI/ASHRAE/IESNA Standard 90.1-2007(ASHRAE Standard 90.1-2007, Standard 90.1-2007, or 2007 edition). The preliminary analysis considered each of the 109 addenda to ASHRAE Standard 90.1-2007 that were included in ASHRAE Standard 90.1-2010. All 109 addenda processed by ASHRAE in the creation of Standard 90.1-2010 from Standard 90.1-2007 were reviewed by DOE, and their combined impact on a suite of 16 building prototype models in 15 ASHRAE climate zones was considered. Most addenda were deemed to have little quantifiable impact on building efficiency for the purpose of DOE’s preliminary determination. However, out of the 109 addenda, 34 were preliminarily determined to have measureable and quantifiable impact.

  4. Graph-based analysis of kinetics on multidimensional potential-energy surfaces.

    Science.gov (United States)

    Okushima, T; Niiyama, T; Ikeda, K S; Shimizu, Y

    2009-09-01

    The aim of this paper is twofold: one is to give a detailed description of an alternative graph-based analysis method, which we call saddle connectivity graph, for analyzing the global topography and the dynamical properties of many-dimensional potential-energy landscapes and the other is to give examples of applications of this method in the analysis of the kinetics of realistic systems. A Dijkstra-type shortest path algorithm is proposed to extract dynamically dominant transition pathways by kinetically defining transition costs. The applicability of this approach is first confirmed by an illustrative example of a low-dimensional random potential. We then show that a coarse-graining procedure tailored for saddle connectivity graphs can be used to obtain the kinetic properties of 13- and 38-atom Lennard-Jones clusters. The coarse-graining method not only reduces the complexity of the graphs, but also, with iterative use, reveals a self-similar hierarchical structure in these clusters. We also propose that the self-similarity is common to many-atom Lennard-Jones clusters.

  5. Thermodynamic Analysis of Chemically Reacting Mixtures and Their Kinetics: Example of a Mixture of Three Isomers.

    Science.gov (United States)

    Pekař, Miloslav

    2016-10-18

    Thermodynamics provides consequences of and restrictions on chemically reacting mixtures, particularly their kinetics, which have not been fully explored. Herein, a comprehensive thermodynamic analysis is illustrated for a reacting mixture of three isomers. The rate equation is first derived on the basis of the results of nonequilibrium continuum thermodynamics of linear fluids, and is then subjected to the requirement of consistency with entropic inequality (the second law). This consistency test involves the correct representation of the reaction rate as a function of affinities. It is shown that entropic inequality restricts the signs or values of coefficients in the constitutive equations for reaction rates/rate constants. The use of reverse rate constants and the identification of thermodynamic and kinetic equilibrium constants are not necessary in this approach. Although the presented thermodynamic analysis works only for independent reactions, the rates of dependent reactions are not excluded from having effects on kinetics. It is shown that the rates of dependent reactions are combined from the rates of independent reactions differently than dependent reactions are combined from independent reactions. The results are compared to the classical mass-action rate equations, and new restrictions on the values of the classical rate constants are derived.

  6. Development of patient collation system by kinetic analysis for chest dynamic radiogram with flat panel detector

    Science.gov (United States)

    Tsuchiya, Yuichiro; Kodera, Yoshie

    2006-03-01

    In the picture archiving and communication system (PACS) environment, it is important that all images be stored in the correct location. However, if information such as the patient's name or identification number has been entered incorrectly, it is difficult to notice the error. The present study was performed to develop a system of patient collation automatically for dynamic radiogram examination by a kinetic analysis, and to evaluate the performance of the system. Dynamic chest radiographs during respiration were obtained by using a modified flat panel detector system. Our computer algorithm developed in this study was consisted of two main procedures, kinetic map imaging processing, and collation processing. Kinetic map processing is a new algorithm to visualize a movement for dynamic radiography; direction classification of optical flows and intensity-density transformation technique was performed. Collation processing consisted of analysis with an artificial neural network (ANN) and discrimination for Mahalanobis' generalized distance, those procedures were performed to evaluate a similarity of combination for the same person. Finally, we investigated the performance of our system using eight healthy volunteers' radiographs. The performance was shown as a sensitivity and specificity. The sensitivity and specificity for our system were shown 100% and 100%, respectively. This result indicated that our system has excellent performance for recognition of a patient. Our system will be useful in PACS management for dynamic chest radiography.

  7. Non-Isothermal Kinetic Analysis of the Crystallization of Metallic Glasses Using the Master Curve Method

    Directory of Open Access Journals (Sweden)

    Jürgen Eckert

    2011-12-01

    Full Text Available The non-isothermal transformation rate curves of metallic glasses are analyzed with the Master Curve method grounded in the Kolmogorov-Johnson-Mehl-Avrami theory. The method is applied to the study of two different metallic glasses determining the activation energy of the transformation and the experimental kinetic function that is analyzed using Avrami kinetics. The analysis of the crystallization of Cu47Ti33Zr11Ni8Si1 metallic glassy powders gives Ea = 3.8 eV, in good agreement with the calculation by other methods, and a transformation initiated by an accelerating nucleation and diffusion-controlled growth. The other studied alloy is a Nanoperm-type Fe77Nb7B15Cu1 metallic glass with a primary crystallization of bcc-Fe. An activation energy of Ea = 5.7 eV is obtained from the Master Curve analysis. It is shown that the use of Avrami kinetics is not able to explain the crystallization mechanisms in this alloy giving an Avrami exponent of n = 1.

  8. Preliminary phytochemical analysis and DPPH free radical scavenging activity of Trewia nudiflora Linn. roots and leaves.

    Science.gov (United States)

    Balakrishnan, N; Srivastava, Mayank; Tiwari, Pallavi

    2013-11-01

    Oxidative stress is one of the major causative factors of many chronic and degenerative diseases. Plants have been used in traditional medicine in different parts of world for thousands of years and continue to provide new remedies for human kind. The present study was to investigate the preliminary phytochemical analysis of various extracts of roots and leaves of Trewia nudiflora (Euphorbiaceae) and antioxidant activity by 1,1,diphenyl-2-picryl hydrazyl (DPPH) radical scavenging method. The preliminary phytochemical screening showed the presence of several phytochemicals including alkaloids, glycosides, flavonoids, steroids, phenolic compounds and tannins. The ethanol and aqueous extracts of roots and leaves of Trewia nudiflora showed significant antioxidant activity compared to standard drug ascorbic acid.

  9. A Numerical Procedure for Model Identifiability Analysis Applied to Enzyme Kinetics

    DEFF Research Database (Denmark)

    Daele, Timothy, Van; Van Hoey, Stijn; Gernaey, Krist;

    2015-01-01

    exercise, thereby bypassing the challenging task of model structure determination and identification. Parameter identification problems can thus lead to ill-calibrated models with low predictive power and large model uncertainty. Every calibration exercise should therefore be precededby a proper model...... and Pronzato (1997) and which can be easily set up for any type of model. In this paper the proposed approach is applied to the forward reaction rate of the enzyme kinetics proposed by Shin and Kim(1998). Structural identifiability analysis showed that no local structural model problems were occurring......The proper calibration of models describing enzyme kinetics can be quite challenging. In the literature, different procedures are available to calibrate these enzymatic models in an efficient way. However, in most cases the model structure is already decided on prior to the actual calibration...

  10. Analysis for the Sorption Kinetics of Ag Nanoparticles on Natural Clinoptilolite

    Directory of Open Access Journals (Sweden)

    Alvaro Ruíz-Baltazar

    2015-01-01

    Full Text Available The kinetic adsorption behavior of silver nanoparticles deposited on a natural zeolite from Oaxaca is presented. Theoretical models as Lagergren first-order, pseudo-second-order, Elovich, and intraparticle diffusion were employed and compared with experimental data obtained by atomic absorption spectrophotometry technique. Correlation factors R2 of the order of 0.99 were observed. Analysis by transmission electron microscopy revealed that the silver nanoparticles were homogeneously distributed on the zeolite. Additionally, chemical characterization of the material was carried out through a dilution process with lithium metaborate. An average value of 9.3 in the Si/Al ratio was observed and related to the kinetic adsorption behavior of the zeolite.

  11. Anion exchange kinetics of nanodimensional layered metal hydroxides: use of isoconversional analysis.

    Science.gov (United States)

    Majoni, Stephen; Hossenlopp, Jeanne M

    2010-12-16

    Anion exchange reactions of nanodimensional layered metal hydroxide compounds are utilized to create materials with targeted physical and chemical properties and also as a means for controlled release of intercalated anions. The kinetics of this important class of reaction are generally characterized by model-based approaches. In this work, a different approach based on isothermal, isoconversional analysis was utilized to determine effective activation energies with respect to extent of reaction. Two different layered metal hydroxide materials were chosen for reaction with chloride anions, using a temperature range of 30-60 °C. The concentrations of anions released into solution and the changes in polycrystalline solid phases were evaluated using model-based (Avrami-Erofe'ev nucleation-growth model) and model-free (integral isoconversional) methods. The results demonstrate the utility of the isoconversional approach for identifying when fitting to a single model is not appropriate, particularly for characterizing the temperature dependence of the reaction kinetics.

  12. Kinetic analysis of artificial peptide self-replication. Part I: the homochiral case.

    Science.gov (United States)

    Islas, Jesús Rivera; Pimienta, Véronique; Micheau, Jean-Claude; Buhse, Thomas

    2003-03-25

    Computational kinetic analysis of a lately discovered homochiral peptide self-replicator is presented. A 6-step kinetic model was designed that addresses the main reactions and hydrophobic interactions involved in this template-directed, autocatalytic system and that gave rise to excellent fitting of 4 previously published independent experimental series. The model sheds light on the mechanistic principle of the reaction system and illustrates directly a number of dynamic properties such as the observed autocatalytic efficiency. It was found that the dynamics are basically governed by two reversible hydrophobic interactions: between the template and a peptide fragment and between two template species. The later association was determined to be considerably more favored, which leads to the predominant presence of the catalytically inactive template dimer in the reaction system. Our results show that the involvement of a template trimer is not necessary to obtain the observed fittings.

  13. Color changes in wood during heating: kinetic analysis by applying a time-temperature superposition method

    Science.gov (United States)

    Matsuo, Miyuki; Yokoyama, Misao; Umemura, Kenji; Gril, Joseph; Yano, Ken'ichiro; Kawai, Shuichi

    2010-04-01

    This paper deals with the kinetics of the color properties of hinoki ( Chamaecyparis obtusa Endl.) wood. Specimens cut from the wood were heated at 90-180°C as accelerated aging treatment. The specimens completely dried and heated in the presence of oxygen allowed us to evaluate the effects of thermal oxidation on wood color change. Color properties measured by a spectrophotometer showed similar behavior irrespective of the treatment temperature with each time scale. Kinetic analysis using the time-temperature superposition principle, which uses the whole data set, was successfully applied to the color changes. The calculated values of the apparent activation energy in terms of L *, a *, b *, and Δ E^{*}_{ab} were 117, 95, 114, and 113 kJ/mol, respectively, which are similar to the values of the literature obtained for other properties such as the physical and mechanical properties of wood.

  14. Kinetic analysis of high-concentration isopropanol biodegradation by a solvent-tolerant mixed microbial culture.

    Science.gov (United States)

    Bustard, Mark T; Meeyoo, Vissanu; Wright, Phillip C

    2002-06-20

    The ability of a previously enriched microbial population to utilize isopropanol (IPA) as the sole carbon source within a minimal salts medium is studied. The advantage of prior enrichment procedures for the improvement of IPA biodegradation performance is demonstrated for an IPA concentration of up to 24 g L(-1). Results showing the interrelationship between temperature and substrate utilization and inhibition levels at temperatures of between 2 degrees C and 45 degrees C are examined. Models of inhibition based on enzyme kinetics are assessed via nonlinear analysis, in order to accurately represent the growth kinetics of this solvent-tolerant mixed culture. The model that best describes the data is the Levenspiel substrate inhibition model, which can predict the maximum substrate level above which growth is completely limited. This is the first report of IPA treatment of up to 24 g L(-1) by an aerobic solvent-tolerant population.

  15. Analysis of proton exchange kinetics with time-dependent exchange rate.

    Science.gov (United States)

    Rutkowska-Wlodarczyk, Izabela; Kierdaszuk, Borys; Wlodarczyk, Jakub

    2010-04-01

    Mass spectrometry is used to probe the kinetics of hydrogen-deuterium exchange in lysozyme in pH 5, 6 and 7.4. An analysis based on a Verhulst growth model is proposed and effectively applied to the kinetics of the hydrogen exchange. The data are described by a power-like function which is based on a time-dependence of the exchange rate. Experimental data ranging over many time scales is considered and accurate fits of a power-like function are obtained. Results of fittings show correlation between faster hydrogen-deuterium exchange and increase of pH. Furthermore a model is presented that discriminates between easily exchangeable hydrogens (located in close proximity to the protein surface) and those protected from the exchange (located in the protein interior). A possible interpretation of the model and its biological significance are discussed.

  16. Ligand-receptor binding kinetics in surface plasmon resonance cells: A Monte Carlo analysis

    CERN Document Server

    Carroll, Jacob; Forsten-Williams, Kimberly; Täuber, Uwe C

    2016-01-01

    Surface plasmon resonance (SPR) chips are widely used to measure association and dissociation rates for the binding kinetics between two species of chemicals, e.g., cell receptors and ligands. It is commonly assumed that ligands are spatially well mixed in the SPR region, and hence a mean-field rate equation description is appropriate. This approximation however ignores the spatial fluctuations as well as temporal correlations induced by multiple local rebinding events, which become prominent for slow diffusion rates and high binding affinities. We report detailed Monte Carlo simulations of ligand binding kinetics in an SPR cell subject to laminar flow. We extract the binding and dissociation rates by means of the techniques frequently employed in experimental analysis that are motivated by the mean-field approximation. We find major discrepancies in a wide parameter regime between the thus extracted rates and the known input simulation values. These results underscore the crucial quantitative importance of s...

  17. Kinetic analysis of RNA interference for lamin A/C in HeLa cells

    Institute of Scientific and Technical Information of China (English)

    Sung-Ju You; Sang-Hoon Lee; Jae-Seung Lee

    2010-01-01

    We kinetically analyzed RNA interference(RNAi)for lamin A/C in HeLa cells,assuming suppression and recovery phases of gene expression,and dilution of transfected small interfering RNA(siRNA)by cell divisions.We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations,and the maximum gene silencing efficiency occurred at Day 2 or 3.The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method,demonstrating that RNAi for lamin A/C can be understood as a conventional drug response system.This work provides potentially important guidelines for future applications using nanomater ials as delivery vehicles of siRNA in RNAi for lamin A/C.

  18. Thermal Analysis On The Kinetics Of Magnesium-Aluminum Layered Double Hydroxides In Different Heating Rates

    Directory of Open Access Journals (Sweden)

    Hongbo Y.

    2015-06-01

    Full Text Available The thermal decomposition of magnesium-aluminum layered double hydroxides (LDHs was investigated by thermogravimetry analysis and differential scanning calorimetry (DSC methods in argon environment. The influence of heating rates (including 2.5, 5, 10, 15 and 20K/min on the thermal behavior of LDHs was revealed. By the methods of Kissinger and Flynn-Wall-Ozawa, the thermal kinetic parameters of activation energy and pre-exponential factor for the exothermic processes under non-isothermal conditions were calculated using the analysis of corresponding DSC curves.

  19. CONSTRUCTION OF THE CHINESE LEARNERS' PARALLEL CORPUS OF JAPANESE AND ITS PRELIMINARY ANALYSIS

    Directory of Open Access Journals (Sweden)

    Masatake Dantsuji

    2004-01-01

    Full Text Available This study aims to introduce the project to construct the Chinese learners' corpus (LC of Japanese at Dalian University of Technology (DUT, and detail the LC construction, development of DUT Corpus Linguistics Tools, and contribution to the education of Japanese as a second language. The outstanding characteristic of the LC is its parallel form with learners' Japanese texts and their Chinese translation, which enables us to make comprehensive analysis of the influence of Chinese (L1 to Japanese (L2. We have made a preliminary analysis of the errors contained.

  20. Preliminary Design and Analysis of the ARES Atmospheric Flight Vehicle Thermal Control System

    Science.gov (United States)

    Gasbarre, J. F.; Dillman, R. A.

    2003-01-01

    The Aerial Regional-scale Environmental Survey (ARES) is a proposed 2007 Mars Scout Mission that will be the first mission to deploy an atmospheric flight vehicle (AFV) on another planet. This paper will describe the preliminary design and analysis of the AFV thermal control system for its flight through the Martian atmosphere and also present other analyses broadening the scope of that design to include other phases of the ARES mission. Initial analyses are discussed and results of trade studies are presented which detail the design process for AFV thermal control. Finally, results of the most recent AFV thermal analysis are shown and the plans for future work are discussed.

  1. Multidisciplinary High-Fidelity Analysis and Optimization of Aerospace Vehicles. Part 2; Preliminary Results

    Science.gov (United States)

    Walsh, J. L.; Weston, R. P.; Samareh, J. A.; Mason, B. H.; Green, L. L.; Biedron, R. T.

    2000-01-01

    An objective of the High Performance Computing and Communication Program at the NASA Langley Research Center is to demonstrate multidisciplinary shape and sizing optimization of a complete aerospace vehicle configuration by using high-fidelity finite-element structural analysis and computational fluid dynamics aerodynamic analysis in a distributed, heterogeneous computing environment that includes high performance parallel computing. A software system has been designed and implemented to integrate a set of existing discipline analysis codes, some of them computationally intensive, into a distributed computational environment for the design of a high-speed civil transport configuration. The paper describes both the preliminary results from implementing and validating the multidisciplinary analysis and the results from an aerodynamic optimization. The discipline codes are integrated by using the Java programming language and a Common Object Request Broker Architecture compliant software product. A companion paper describes the formulation of the multidisciplinary analysis and optimization system.

  2. Kinetic Analysis of Competitive Electrocatalytic Pathways: New Insights into Hydrogen Production with Nickel Electrocatalysts

    Energy Technology Data Exchange (ETDEWEB)

    Wiedner, Eric S.; Brown, Houston J.; Helm, Monte L.

    2016-01-20

    The hydrogen production electrocatalyst Ni(PPh2NPh2)22+ (1) is capable of traversing multiple electrocatalytic pathways. When using dimethylformamidium, DMF(H)+, the mechanism of formation of H2 catalyzed by 1 changes from an ECEC to an EECC mechanism as the potential approaches the Ni(I/0) couple. Two recent electrochemical methods, current-potential analysis and foot-of-the-wave analysis (FOWA), were performed on 1 to measure the detailed chemical kinetics of the competing ECEC and EECC pathways. A sensitivity analysis was performed on the electrochemical methods using digital simulations to gain a better understanding of their strengths and limitations. Notably, chemical rate constants were significantly underestimated when not accounting for electron transfer kinetics, even when electron transfer was fast enough to afford a reversible non-catalytic wave. The EECC pathway of 1 was found to be faster than the ECEC pathway under all conditions studied. Using buffered DMF: DMF(H)+ mixtures led to an increase in the catalytic rate constant (kobs) of the EECC pathway, but kobs for the ECEC pathway did not change when using buffered acid. Further kinetic analysis of the ECEC path revealed that added base increases the rate of isomerization of the exo-protonated Ni(0) isomers to the catalytically active endo-isomers, but decreases the net rate of protonation of Ni(I). FOWA on 1 did not provide accurate rate constants due to incomplete reduction of the exo-protonated Ni(I) intermediate at the foot of the wave, but FOWA could be used to estimate the reduction potential of this previously undetected intermediate. This research was supported as part of the Center for Molecular Electrocatalysis, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences. Pacific Northwest National Laboratory is operated by Battelle for the U.S. Department of Energy.

  3. Comparison of dynamic 2D and 3D brain PET for kinetic analysis of C-11 win 35,428 binding to dopamine transporters

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Sang Eun; Choi, Joon Young; Choi, Yong; Kim, Joon Young; Choe, Yearn Seong; Oh, Seung Jun; Kim, Byung Tae [College of Medicine, Sungkyunkwan Univ., Seoul (Korea, Republic of)

    1998-07-01

    3D PET data acquisition is now being used for clinical FDG-PET brain studies. However, its validity for studying kinetics of receptor-ligand interaction has not been fully evaluated. To examine the feasibility and quantitative accuracy of dynamic 3D brain PET for kinetic analysis of radioligand binding, 4 healthy volunteers and 1 Parkinson's disease patient were studied. Each subject received two 555 MBq C-11 WIN 35,428 injections and a dynamic sequence of 31 scans were acquired until 90 min p.i. in 2D and 3D mode using a GE Advance PET scanner. Both 2D and 3D image sets were reconstructed employing a Hanning filter 4.5 mm and resulting axial resolution was about 7 mm for both 2D and 3D images. The 3D data were corrected for scatter employing a method using 2D fitted Gaussian functions. Attenuation correction was performed using a 20 min transmission scan. Tissue time-activity curves were generated in the striatum and cerebellum. The forward (k3) and dissociation (k4) rate constants were calculated in the striatum using a two-compartment model, which consists of free plus nonspecifically bound (cerebellar) and specifically bound (striatal-cerebellar) compartments. The 3D kinetic results were comparable to the 2D results and within the expected range. The 3D %SE was less than 2D %SE. Striatal-to-cerebellar ratios from 2D and 3D images showed excellent correlation and agreement (r=0.994, p<0.0001, slope=0.952). The plot of striatal-to-cerebellar ratios versus time showed a linearly increasing pattern, with significantly lower RMSE in 3D than in 2D studies in linear regression analysis (0.119{+-}0.025 vs. 0.276{+-}0.087, p=0.028). These preliminary data suggest that 3D PET provides more reliable tissue kinetic data for the analysis of C-11 WIN 35,428 kinetics. Improved sensitivity in 3D may allow more accurate receptor characterization, especially in small brain structures or in low specific binding areas.

  4. Waste Feed Delivery System Phase 1 Preliminary RAM Analysis [SEC 1 and 2

    Energy Technology Data Exchange (ETDEWEB)

    DYKES, A.A.

    2000-10-11

    This report presents the updated results of the preliminary reliability, availability, and maintainability (RAM) analysis of selected waste feed delivery (WFD) operations to be performed by the Tank Farm Contractor (TFC) during Phase I activities in support of the Waste Treatment and Immobilization Plant (WTP). For planning purposes, waste feed tanks are being divided into five classes in accordance with the type of waste in each tank and the activities required to retrieve, qualify, and transfer waste feed. This report reflects the baseline design and operating concept, as of the beginning of Fiscal Year 2000, for the delivery of feed from three of these classes, represented by source tanks 241-AN-102, 241-AZ-101 and 241-AN-105. The preliminary RAM analysis quantifies the potential schedule delay associated with operations and maintenance (OBM) field activities needed to accomplish these operations. The RAM analysis is preliminary because the system design, process definition, and activity planning are in a state of evolution. The results are being used to support the continuing development of an O&M Concept tailored to the unique requirements of the WFD Program, which is being documented in various volumes of the Waste Feed Delivery Technical Basis (Carlson. 1999, Rasmussen 1999, and Orme 2000). The waste feed provided to the WTP must: (1) meet limits for chemical and radioactive constituents based on pre-established compositional envelopes (i.e., feed quality); (2) be in acceptable quantities within a prescribed sequence to meet feed quantities; and (3) meet schedule requirements (i.e., feed timing). In the absence of new criteria related to acceptable schedule performance due to the termination of the TWRS Privatization Contract, the original criteria from the Tank Waste Remediation System (77443s) Privatization Contract (DOE 1998) will continue to be used for this analysis.

  5. Dynamic nuclear renography kinetic analysis: Four-compartment model for assessing kidney function

    Energy Technology Data Exchange (ETDEWEB)

    Raswan, T. R., E-mail: tara.raissa@gmail.com; Haryanto, F., E-mail: tara.raissa@gmail.com [Department of Physics, Nuclear Physics and Biophysics Research Group, Institut Teknologi Bandung, Bandung (Indonesia)

    2014-09-30

    Dynamic nuclear renography method produces TACs of kidneys and bladder. Multiple TACs data can be further analyzed to obtain the overview of urinary system's condition. Tracer kinetic analysis was performed using four-compartment models. The system's model consist of four irreversible compartment with four transport constants (k1, k2, k3 and k4). The mathematical expressions of tracer's distributions is fitted to experimental data (TACs) resulting in model constants. This transport constants represent the urinary system behavior, and later can be used for analyzing system's condition. Different intervals of kinetics parameter are clearly shown by abnormal system with respect to the normal one. Furthermore, the system with delayed uptake has 82% lower uptake parameters (k1 and k2) than normal one. Meanwhile, the system with prolonged clearance time has its kinetics parameters k3 or k4 lower than the others. This model is promising for quantitatively describe urinary system's function especially if supplied with more data.

  6. Iso-conversional kinetic analysis of quaternary glass re-crystallization.

    Science.gov (United States)

    Srivastava, Ankita; Chandel, Namrata; Mehta, Neeraj

    2017-02-01

    Iso-conversional kinetic analysis is popular in scientific community for analyzing solid-state reactions (e.g., glass/amorphous and amorphous/crystal phase transformations, re-crystallization etc). It is a recognized significant tool to achieve useful outcomes for the solid state reaction under consideration. Present work is devoted to explore some insights of thermally activated crystallization using various heating rates (VHR) method. We have examined the correlation between iso-conversional activation energy and iso-conversional rate of crystal growth. In fact, we have observed the compensation law and iso-kinetic relationship using two different approaches for the study of crystallization phenomenon that drives thermally in an Arrhenian manner. Moreover, we found that the estimated intercepts and gradients (i.e., Meyer-Neldel energy and Meyer-Neldel pre-factor respectively) for both approaches also vary linearly and both sets are remarkably identical. These results approach to an inference for ensuring the equivalence of compensation law and iso-kinetic relationship and provide an understanding of various advanced materials in physical chemistry, materials sciences and solid-state physics.

  7. Theoretical analysis of the kinetic performance of laboratory- and full-scale composting systems.

    Science.gov (United States)

    Baptista, Marco; Silveira, Ana; Antunes, Fernando

    2012-07-01

    Composting research at laboratory-scale is critical for the development of optimized full-scale plants. Discrepancies between processes at laboratory-scale and full-scale systems have been investigated in terms of heat balances, but a kinetic analysis of this issue is still missing. In this study, the composting rate at laboratory-scale was, on average, between 1.9 and 5.7 times faster than in full-scale systems for a set of published studies using municipal solid waste, food waste or similar materials. Laboratory-scale performance and full-scale systems were limited to 71 and 46%, respectively, of their maximum potential due to poor management of environmental process conditions far from their optimum. The main limiting environmental factor was found to be moisture content, followed by temperature. Besides environmental factors, waste composition and particle size were identified as factors accounting for kinetic differences between laboratory- and full-scale systems. Overall, this study identifies those factors that affect the kinetics of the composting process most and revealed a significant margin for reducing process time in full-scale composting.

  8. Curing kinetics of visible light curing dental resin composites investigated by dielectric analysis (DEA).

    Science.gov (United States)

    Steinhaus, Johannes; Hausnerova, Berenika; Haenel, Thomas; Großgarten, Mandy; Möginger, Bernhard

    2014-03-01

    During the curing process of light curing dental composites the mobility of molecules and molecule segments is reduced leading to a significant increase of the viscosity as well as the ion viscosity. Thus, the kinetics of the curing behavior of 6 different composites was derived from dielectric analysis (DEA) using especially redesigned flat sensors with interdigit comb electrodes allowing for irradiation at the top side and measuring the ion viscosity at the bottom side. As the ion viscosities of dental composites change 1-3 orders of magnitude during the curing process, DEA provides a sensitive approach to evaluate their curing behavior, especially in the phase of undisturbed chain growth. In order to determine quantitative kinetic parameters a kinetic model is presented and examined for the evaluation of the ion viscosity curves. From the obtained results it is seen that DEA might be employed in the investigation of the primary curing process, the quality assurance of ingredients as well as the control of processing stability of the light curing dental composites. Copyright © 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  9. Solvent-induced lysozyme gels: rheology, fractal analysis, and sol-gel kinetics.

    Science.gov (United States)

    da Silva, Marcelo A; Arêas, Elizabeth P G

    2005-09-15

    In this work, the gelation kinetics and fractal character of lysozyme gel matrices developed in tetramethylurea (TMU)-water media were investigated. Gelation times were determined from the temporal crossover point between the storage, G', and loss, G'', moduli, as a function of the binary solvent composition and of protein concentration. The inverse dependence of the upper limit of the linear viscoelastic region (gamma0) on protein concentration indicate that the lysozyme gels belong to the "strong link" kind, a gel category where interparticle links are stronger than intraparticle ones. Lysozyme gel fractal dimensions (Df) were determined from the analysis of rheological data according to a scaling theory by Shih et al. [Phys. Rev. A 42 (1990) 4772-4779] and were found to be compatible with a diffusion-limited cluster-aggregation kinetics (DLCA) for lysozyme gels formed at the TMU mass fraction in the binary organic-aqueous solvent, wTMU=0.9, and with a reaction-limited cluster aggregation kinetics (RLCA) for wTMU in the 0.6< or =wTMU< or =0.8 range.

  10. Analysis of a kinetic multi-segment foot model. Part I: Model repeatability and kinematic validity.

    Science.gov (United States)

    Bruening, Dustin A; Cooney, Kevin M; Buczek, Frank L

    2012-04-01

    Kinematic multi-segment foot models are still evolving, but have seen increased use in clinical and research settings. The addition of kinetics may increase knowledge of foot and ankle function as well as influence multi-segment foot model evolution; however, previous kinetic models are too complex for clinical use. In this study we present a three-segment kinetic foot model and thorough evaluation of model performance during normal gait. In this first of two companion papers, model reference frames and joint centers are analyzed for repeatability, joint translations are measured, segment rigidity characterized, and sample joint angles presented. Within-tester and between-tester repeatability were first assessed using 10 healthy pediatric participants, while kinematic parameters were subsequently measured on 17 additional healthy pediatric participants. Repeatability errors were generally low for all sagittal plane measures as well as transverse plane Hindfoot and Forefoot segments (mediansegment rigidity analysis suggested rigid body behavior for the Shank and Hindfoot, with the Forefoot violating the rigid body assumptions in terminal stance/pre-swing. Joint excursions were consistent with previously published studies.

  11. Fast and sensitive optical toxicity bioassay based on dual wavelength analysis of bacterial ferricyanide reduction kinetics.

    Science.gov (United States)

    Pujol-Vila, F; Vigués, N; Díaz-González, M; Muñoz-Berbel, X; Mas, J

    2015-05-15

    Global urban and industrial growth, with the associated environmental contamination, is promoting the development of rapid and inexpensive general toxicity methods. Current microbial methodologies for general toxicity determination rely on either bioluminescent bacteria and specific medium solution (i.e. Microtox(®)) or low sensitivity and diffusion limited protocols (i.e. amperometric microbial respirometry). In this work, fast and sensitive optical toxicity bioassay based on dual wavelength analysis of bacterial ferricyanide reduction kinetics is presented, using Escherichia coli as a bacterial model. Ferricyanide reduction kinetic analysis (variation of ferricyanide absorption with time), much more sensitive than single absorbance measurements, allowed for direct and fast toxicity determination without pre-incubation steps (assay time=10 min) and minimizing biomass interference. Dual wavelength analysis at 405 (ferricyanide and biomass) and 550 nm (biomass), allowed for ferricyanide monitoring without interference of biomass scattering. On the other hand, refractive index (RI) matching with saccharose reduced bacterial light scattering around 50%, expanding the analytical linear range in the determination of absorbent molecules. With this method, different toxicants such as metals and organic compounds were analyzed with good sensitivities. Half maximal effective concentrations (EC50) obtained after 10 min bioassay, 2.9, 1.0, 0.7 and 18.3 mg L(-1) for copper, zinc, acetic acid and 2-phenylethanol respectively, were in agreement with previously reported values for longer bioassays (around 60 min). This method represents a promising alternative for fast and sensitive water toxicity monitoring, opening the possibility of quick in situ analysis.

  12. Improved neutron kinetics for coupled three-dimensional boiling water reactor analysis

    Science.gov (United States)

    Akdeniz, Bedirhan

    The need for a more accurate method of modelling cross section variations for off-nominal core conditions is becoming an important issue with the increased use of coupled three-dimensional (3-D) thermal-hydraulics/neutronics simulations. In traditional reactor core analysis, thermal reactor core calculations are customarily performed with 3-D two-group nodal diffusion methods. Steady-state multi-group transport theory calculations on heterogeneous single assembly domains subject to reflective boundary conditions are normally used to prepare the equivalent two-group spatially homogenized nodal parameters. For steady-state applications, the equivalent nodal parameters are theoretically well-defined; but, for transient applications, the definition of the nodal kinetics parameters, in particular, delayed neutron precursor data is somewhat unclear. The fact that delayed neutrons are emitted at considerably lower energies than prompt neutrons and that this difference cannot be accounted for in a two-group representation is of particular concern. To compensate for this inherent deficiency of the two-group model a correction is applied to the nodal values of the delayed neutron fractions; however, the adequacy of this correction has never been tested thoroughly for Boiling Water Reactor (BWR) applications, especially where the instantaneous thermal-hydraulic conditions play an important role on the core neutron kinetics calculations. This thesis proposes a systematic approach to improve the 3-D neutron kinetics modelling in coupled BWR transient calculations by developing, implementing and validating methods for consistent generation of neutron kinetics and delayed neutron data for such coupled thermal-hydraulics/neutronics simulations.

  13. Chromatographic preparation and kinetic analysis of interactions between tabun enantiomers and acetylcholinesterase.

    Science.gov (United States)

    Tenberken, O; Thiermann, H; Worek, F; Reiter, G

    2010-06-02

    The easy accessibility to highly toxic OP (organophosphorus)-type chemical warfare agents (nerve agents) underlines the necessity for an effective medical treatment. Acute OP toxicity is primarily caused by inhibition of acetylcholinesterase (AChE, EC 3.1.1.7). Reactivators (oximes) of inhibited AChE are a mainstay of treatment. However, the commercially available compounds, obidoxime and pralidoxime, are considered rather ineffective against various nerve agents, including tabun. OP-type chemical warfare agents include an asymmetrical P-atom and consist of at least two stereoisomers. Previous studies with the nerve agents sarin and soman showed marked differences between (-)- and (+)-P isomers regarding AChE inhibition and stability in biological matrices. Hence, stereoselectivity is a key parameter for the development of optimized treatment. In the present study, the tabun enantiomers were isolated by semi-preparative liquid-chromatography (LC) with offline analysis by GC-PCI-MS and final characterization of optical purity (99.98% (-)-tabun and 99.83% (+)-tabun) and specific optical rotation. The inhibition and reactivation kinetics of the tabun enantiomers were determined with human and swine AChE and the aging kinetics with human AChE. The results show a large difference in the inhibitory potency between (-)- and (+)-tabun. The determination of reactivation and aging kinetics indicates that both reactions are at least in part determined by the residual (-)-tabun contamination (0.17%) of the (+)-tabun preparation. These data provide further insight into the kinetic interactions between tabun enantiomers and AChE and may contribute to the development of more effective treatment options.

  14. Synthetic spectral analysis of a kinetic model for slow-magnetosonic waves in solar corona

    Energy Technology Data Exchange (ETDEWEB)

    Ruan, Wenzhi; He, Jiansen; Tu, Chuanyi; Wang, Linghua [School of Earth and Space Sciences, Peking University, Beijing, 100871, China, E-mail: jshept@gmail.com (China); Zhang, Lei [State Key Laboratory of Space Weather, Chinese Academy of Sciences, Beijing 100190 (China); Vocks, Christian [Leibniz-Institut für Astrophysik Potsdam, 14482, Potsdam (Germany); Marsch, Eckart [Institute for Experimental and Applied Physics, Christian-Albrechts-Universität zu Kiel, 24118 Kiel (Germany); Peter, Hardi [Max Plank Institut für Sonnensystemforschung, Justus-von-Liebig-Weg 3, 37077 Göttingen (Germany)

    2016-03-25

    We propose a kinetic model of slow-magnetosonic waves to explain various observational features associated with the propagating intensity disturbances (PIDs) occurring in the solar corona. The characteristics of slow mode waves, e.g, inphase oscillations of density, velocity, and thermal speed, are reproduced in this kinetic model. Moreover, the red-blue (R-B) asymmetry of the velocity distribution as self-consistently generated in the model is found to be contributed from the beam component, as a result of the competition between Landau resonance and Coulomb collisions. Furthermore, we synthesize the spectral lines and make the spectral analysis, based on the kinetic simulation data of the flux tube plasmas and the hypothesis of the surrounding background plasmas. It is found that the fluctuations of parameters of the synthetic spectral lines are basically consistent with the observations: (1) the line intensity, Doppler shift, and line width are fluctuating in phase; (2) the R-B asymmetry usually oscillate out of phase with the former three parameters; (3) the blueward asymmetry is more evident than the redward asymmetry in the R-B fluctuations. The oscillations of line parameters become weakened for the case with denser surrounding background plasmas. Similar to the observations, there is no doubled-frequency oscillation of the line width for the case with flux-tube plasmas flowing bulkly upward among the static background plasmas. Therefore, we suggest that the “wave + beam flow” kinetic model may be a viable interpretation for the PIDs observed in the solar corona.

  15. Blood, urine, and hair kinetic analysis following an acute lead intoxication.

    Science.gov (United States)

    Ho, G; Keutgens, A; Schoofs, R; Kotolenko, S; Denooz, R; Charlier, C

    2011-01-01

    A case of lead exposure resulting from the accidental ingestion of a lead-containing solution is reported. Because of clinical management rapidly performed through chelation therapy by 2,3-dimercaptopropane sulfonate sodium and meso-2,3-dimercaptosuccinic acid, blood lead levels of this 51-year-old patient were moderate (412.9 μg/L) and no clinical symptoms were observed. Numerous blood and urine samples were collected for kinetic analysis of lead elimination. However, we report the first case in which hair samples were analyzed to determine the excretion level of lead after acute intoxication.

  16. Analysis of the kinetic mechanism of recombinant human isoprenylcysteine carboxylmethyltransferase (Icmt

    Directory of Open Access Journals (Sweden)

    Baron Rudi A

    2004-12-01

    Full Text Available Abstract Background Isoprenylcysteine carboxyl methyltransferase (Icmt is the third of three enzymes that posttranslationally modify proteins that contain C-terminal CaaX motifs. The processing of CaaX proteins through this so-called prenylation pathway via a route initiated by addition of an isoprenoid lipid is required for both membrane targeting and function of the proteins. The involvement of many CaaX proteins such as Ras GTPases in oncogenesis and other aberrant proliferative disorders has led to the targeting of the enzymes involved in their processing for therapeutic development, necessitating a detailed understanding of the mechanisms of the enzymes. Results In this study, we have investigated the kinetic mechanism of recombinant human Icmt. In the reaction catalyzed by Icmt, S-adenosyl-L-methionine (AdoMet provides the methyl group that is transferred to the second substrate, the C-terminal isoprenylated cysteine residue of a CaaX protein, thereby generating a C-terminal prenylcysteine methyl ester on the protein. To facilitate the kinetic analysis of Icmt, we synthesized a new small molecule substrate of the enzyme, biotin-S-farnesyl-L-cysteine (BFC. Initial kinetic analysis of Icmt suggested a sequential mechanism for the enzyme that was further analyzed using a dead end competitive inhibitor, S-farnesylthioacetic acid (FTA. Inhibition by FTA was competitive with respect to BFC and uncompetitive with respect to AdoMet, indicating an ordered mechanism with SAM binding first. To investigate the order of product dissociation, product inhibition studies were undertaken with S-adenosyl-L-homocysteine (AdoHcy and the N-acetyl-S-farnesyl-L-cysteine methylester (AFCME. This analysis indicated that AdoHcy is a competitive inhibitor with respect to AdoMet, while AFCME shows a noncompetitive inhibition with respect to BFC and a mixed-type inhibition with respect to AdoMet. These studies established that AdoHcy is the final product released, and

  17. Kinetic analysis of the termal decomposition of colombian vacuum residua by termogravimetry

    Directory of Open Access Journals (Sweden)

    Fabian Andrey Diaz Mateus

    2015-12-01

    Full Text Available Five different Colombian vacuum residues were thermally decomposed in a thermogravimetric analyzer. Three heating rates were employed to heat the sample up to 650°C. The kinetic analysis was performed by the Coats-Redfern method to describe the non-isothermal pyrolysis of the residua, a reaction model where the reaction order gradually increases from first to second order is proposed and an excellent agreement of the experimental with the calculated data is presented. The results also indicate that the pyrolysis of a vacuum residue cannot be modeled by a single reaction mechanism.

  18. Preliminary analysis of Alvito-Odivelas reservoir system operation under climate change scenarios

    OpenAIRE

    2008-01-01

    The present study provides a preliminary analysis of the impact of climate change on a water resources system of Alentejo region in the South of Portugal. Regional climate model HadRM3P forced by the Global Circulation Model HadAM3P A2 of the Hadley Centre, is used to derive temperature and precipitation data, which in turn is used as input to hydrological model (SHETRAN) for simulation of future streamflow. Dynamic programming based models are used for operation of reservoir system in order ...

  19. Stock assessment of Haliporoides triarthrus (Fam. Solenoceridae) off Mozambique: a preliminary analysis

    OpenAIRE

    Torstensen, E.; Pacule, H.

    1992-01-01

    The pink shrimp, Haliporoides triarthrus, is an important species in the deep-water shrimp fishery in Mozambique. Total catches are in the range of 1,500 to 2,700 tons, with the pink shrimp accounting for 70-90%. Estimates of growth parameters and of natural mortality are used for a preliminary assessment of the fishery, based on length-structured virtual population analysis and yield-per-recruit analyses. With an arbitrarily chosen terminal fishing mortality F, the results indicate a situati...

  20. Preliminary Analysis of Liquid Metal MHD Pressure Drop in the Blanket for the FDS

    Institute of Scientific and Technical Information of China (English)

    王红艳; 吴宜灿; 何晓雄

    2002-01-01

    Preliminary analysis and calculation of liquid metal Li17Pb83 magnetohydrodynamic (MHD) pressure drop in the blanket for the FDS have been presented to evaluate the significance of MHD effects on the thermal-hydraulic design of the blanket. To decrease the liquid metal MHD pressure drop, Al2O3 is applied as an electronically insulated coating onto the inner surface of the ducts. The requirement for the insulated coating to reduce the additional leakage pressure drop caused by coating imperfections has been analyzed. Finally, the total liquid metal MHD pressure drop and magnetic pump power in the FDS blanket have been given.

  1. Preliminary performance analysis of a transverse flow spectrally selective two-slab packed bed volumetric receiver

    CSIR Research Space (South Africa)

    Roos, TH

    2016-05-01

    Full Text Available stream_source_info Roos_2016_ABSTRACT.pdf.txt stream_content_type text/plain stream_size 2694 Content-Encoding UTF-8 stream_name Roos_2016_ABSTRACT.pdf.txt Content-Type text/plain; charset=UTF-8 21st SolarPACES... International Conference (SolarPACES 2015), 13-16 October 2015 Preliminary Performance Analysis of a Transverse Flow Spectrally Selective Two-slab Packed Bed Volumetric Receiver Thomas H. Roos1, a) and Thomas M. Harms2, b) 1Aeronautical Systems...

  2. Interaction and kinetic analysis for coal and biomass co-gasification by TG-FTIR.

    Science.gov (United States)

    Xu, Chaofen; Hu, Song; Xiang, Jun; Zhang, Liqi; Sun, Lushi; Shuai, Chao; Chen, Qindong; He, Limo; Edreis, Elbager M A

    2014-02-01

    This study aims to investigate the interaction and kinetic behavior of CO2 gasification of coal, biomass and their blends by thermogravimetry analysis (TG). The gas products evolved from gasification were measured online with Fourier Transform Infrared Spectroscopy (FTIR) coupled with TG. Firstly, TG experiments indicated that interaction between the coals and biomasses mainly occurred during co-gasification process. The most significant synergistic interaction occurred for LN with SD at the blending mass ratio 4:1. Furthermore, thermal kinetic analysis indicated that the activation energy involved in co-gasification decreased as the SD content increased until the blending ratio of SD with coal reached 4:1. The rise of the frequency factor indicated that the increase of SD content favored their synergistic interaction. Finally, FTIR analysis of co-gasification of SD with LN indicated that except for CO, most gases including CH3COOH, C6H5OH, H2O, etc., were detected at around 50-700°C. Copyright © 2014. Published by Elsevier Ltd.

  3. Atomic force microscopy study, kinetic roughening and multifractal analysis of electrodeposited silver films

    Science.gov (United States)

    Nasehnejad, M.; Gholipour Shahraki, M.; Nabiyouni, G.

    2016-12-01

    We used atomic force microscopy (AFM) to study surface morphology and kinetic roughening of Ag films. X-ray diffraction (XRD) technique is used to verify the films crystalline structure. The influence of film thickness on the kinetic roughening was investigated using AFM data and roughness calculation. It is revealed that the surface roughness increases with increasing the film thickness. The data also consist with a complex behavior which is called as anomalous scaling. Scaling laws analysis for Ag films presents two distinct dynamics including large local and scale roughness and indicates a power law dependency on the thickness of film. AFM images have been characterized by the multifractal analysis. This analysis shows that the self-similar and multifractal characteristics as well as anomalous scaling exist in the Ag film morphologies. Description of the quantitative growth and surface morphology was done by the multifractal spectra, f (α) - α . It is found that the multifractal spectrum shape is left hook-like (that is difference of height interval of the multifractal spectrum, Δf = f (αmin) - f (αmax) > 0). The results indicate that the surfaces having greater roughness give rise the wider multifractal spectrum width (Δα) and the greater Δf, thus, the nonuniformity of the height probabilities becomes larger. It indicates that the multifractality of the films becomes more pronounced at the higher thickness.

  4. Preliminary Report: Analysis of the baseline study on the prevalence of Salmonella in laying hen flocks of Gallus gallus

    DEFF Research Database (Denmark)

    Hald, Tine

    This is a preliminary report on the analysis of the Community-wide baseline study to estimate the prevalence of Salmonella in laying hen flocks. It is being published pending the full analysis of the entire dataset from the baseline study. The report contains the elements necessary for the establ......This is a preliminary report on the analysis of the Community-wide baseline study to estimate the prevalence of Salmonella in laying hen flocks. It is being published pending the full analysis of the entire dataset from the baseline study. The report contains the elements necessary...

  5. Cluster analysis in kinetic modelling of the brain: A noninvasive alternative to arterial sampling

    DEFF Research Database (Denmark)

    Liptrot, Matthew George; Adams, K.H.; Martiny, L.

    2004-01-01

    by the 'within-variance' measure and by 3D visual inspection of the homogeneity of the determined clusters. The cluster-determined input curve was then used in Logan plot analysis and compared with the arterial and venous blood samples, and additionally with one of the currently used alternatives to arterial...... acts as a proof-of-principle that the use of cluster analysis on a PET data set could obviate the requirement for arterial cannulation when determining the input function for kinetic modelling of ligand binding, and that this may be a superior approach as compared to the other noninvasive alternatives......) extracted directly from dynamic positron emission tomography (PET) scans by cluster analysis. Five healthy subjects were injected with the 5HT2A- receptor ligand [18F]-altanserin and blood samples were subsequently taken from the radial artery and cubital vein. Eight regions-of-interest (ROI) TACs were...

  6. Synthesis, Spectral, Thermal Analysis, Biological Activity and Kinetic Studies of Copper(II)-Pyridine-2,5-dicarboxylate Complexes with 2-Aminomethylpyridine and 8-Hydroxyquinoline.

    Science.gov (United States)

    Colak, Alper Tolga; Colak, Ferdağ; Atar, Necip; Olgun, Asım

    2010-03-01

    In this work we report the synthesis of two novel square-planar copper(II) complexes, namely, (2-aminomethylpyridinium-pyridinedicarboxylato)copper(II) dihydrate, [Cu(pydc)(2-amp)] • 2H2O (1) and (8-hydroxyquinolinum-pyridinedicarboxylato)copper(II) hydrate, [Cu(pydc)(8-HQ)] • H2O (2) (2-amp = 2-aminomethylpyridine, 8-HQ = 8-hydroxyquinoline, H2pydc = pyridine-2,5-dicarboxylic acid or isocinchomeronic acid) and present the first preliminary study on kinetics and biological activities of copper complexes. The synthesized complexes have been characterized by elemental, spectroscopic (FT-IR, UV and mass spectra), thermal analysis, magnetic and conductivity measurements techniques. Kinetic parameters were obtained for each stage of thermal degradation of the complexes using Coats-Redfern and Horowitz-Metzger methods. Antimicrobial activities of two complexes and two ligands were evaluated using agar diffusion method. Antimicrobial activity of complex 2 was determined with the agar dilution methods. The results were compared with two well known antibiotics, namely, tetracycline and nystatin.

  7. In situ X-ray pair distribution function analysis of geopolymer gel nanostructure formation kinetics.

    Science.gov (United States)

    White, Claire E; Provis, John L; Bloomer, Breaunnah; Henson, Neil J; Page, Katharine

    2013-06-14

    With the ever-increasing environmentally-driven demand for technologically advanced structural materials, geopolymer cement is fast becoming a viable alternative to traditional cements due to its proven engineering characteristics and the reduction in CO2 emitted during manufacturing (as much as 80% less CO2 emitted in manufacture, compared to ordinary Portland cement). Nevertheless, much remains unknown regarding the kinetics of reaction responsible for nanostructural evolution during the geopolymerisation process. Here, in situ X-ray total scattering measurements and pair distribution function (PDF) analysis are used to quantify the extent of reaction as a function of time for alkali-activated metakaolin/slag geopolymer binders, including the impact of various activators (alkali hydroxide/silicate) on the kinetics of the geopolymerisation reaction. Quantifying the reaction process in situ from X-ray PDF data collected during the initial ten hours can provide an estimate of the total reaction extent, but when combined with data obtained at longer times (128 days here) enables more accurate determination of the overall rate of reaction. To further assess the initial stages of the geopolymerisation reaction process, a pseudo-single step first order rate equation is fitted to the extent of reaction data, which reveals important mechanistic information regarding the role of free silica in the activators in the evolution of the binder systems. Hence, it is shown that in situ X-ray PDF analysis is an ideal experimental local structure tool to probe the reaction kinetics of complex reacting systems involving transitions between disordered/amorphous phases, of which geopolymerisation is an important example.

  8. Non-invasive differentiation of pancreatic lesions: is analysis of FDG kinetics superior to semiquantitative uptake value analysis?

    Energy Technology Data Exchange (ETDEWEB)

    Nitzsche, E.U.; Hoegerle, S.; Mix, M.; Brink, I.; Otte, A.; Moser, E. [Freiburg Univ. (Germany). Abt. Radiologie; Imdahl, A. [Division of Visceral Surgery, Department of Surgery, Albert Ludwigs University, Medical Center, Freiburg (Germany)

    2002-02-01

    The diagnostic utility of fluorine-18 2-deoxy-D-glucose positron emission tomography (FDG PET) for the non-invasive differentiation of focal pancreatic lesions originating from cancer or chronic pancreatitis by combined visual image interpretation and semiquantitative uptake value analysis has been documented. However, in clinical routine some misdiagnosis is still observed. This is because there is potential overlap between the semiquantitative uptake values obtained for active inflammatory lesions and cancer. Therefore, this prospective study was undertaken to test the hypothesis that analysis of dynamic kinetics of focal pancreatic lesions based on FDG PET may more accurately determine the benign or malignant nature of such lesions. Thirty patients (56{+-}17 years) were studied dynamically with FDG PET for a period of 60-90 min. Patients were assigned to one of four groups: control, acute pancreatitis, chronic pancreatitis or pancreatic cancer. Two observers, blinded to the clinical data, analysed the time-activity curves of FDG kinetics based on region of interest analysis. The diagnosis predicted by FDG PET was compared with the result of histological examination of the surgical specimen. Analysis of FDG kinetics revealed significant differences in the shape of the time-activity curve for controls, pancreatic cancer and inflammatory disease. Surprisingly, there was no significant difference in the time-activity curve shape for chronic pancreatitis and acute pancreatitis; this is, however, not a clinical issue. Furthermore, acquisition time (60 min vs 90 min) did not affect interpretation of the time-activity curve, so that scanning time may be regularly shortened to 60 min. Interobserver agreement was 1. Based on these findings, non-invasive differentiation between pancreatic cancer and chronic pancreatitis was correctly predicted in all cases, as confirmed by histology. In addition, the specificity was increased compared with that obtained from standardised

  9. Preliminary phytochemical screening, Antibacterial potential and GC-MS analysis of two medicinal plant extracts.

    Science.gov (United States)

    Vijayaram, Seerangaraj; Kannan, Suruli; Saravanan, Konda Mani; Vasantharaj, Seerangaraj; Sathiyavimal, Selvam; P, Palanisamy Senthilkumar

    2016-05-01

    The presence study was aimed to catalyze the primary metabolites and their confirmation by using GC-MS analysis and antibacterial potential of leaf extract of two important medicinal plant viz., Eucalyptus and Azadirachta indica. The antibacterial potential of the methanol leaf extract of the studied species was tested against Escherichia coli, Pseudomonas aeruginosa, Klebsiellap neumoniae, Streptococcus pyogens, Staphylococcus aureus using by agar well diffusion method. The higher zone of inhibition (16mm) was observed against the bacterium Pseudomonas aeruginosa at 100μl concentration of methanol leaf extract. Preliminary phytochemical analysis of studied species shows that presence of phytochemical compounds like steroids, phenolic compounds and flavonoids. GC-MS analysis confirms the occurrence of 20 different compounds in the methanol leaf extract of the both studied species.

  10. 1972 preliminary safety analysis report based on a conceptual design of a proposed repository in Kansas

    Energy Technology Data Exchange (ETDEWEB)

    Blomeke, J.O.

    1977-08-01

    This preliminary safety analysis report is based on a proposed Federal Repository at Lyons, Kansas, for receiving, handling, and depositing radioactive solid wastes in bedded salt during the remainder of this century. The safety analysis applies to a hypothetical site in central Kansas identical to the Lyons site, except that it is free of nearby salt solution-mining operations and bore holes that cannot be plugged to Repository specifications. This PSAR contains much information that also appears in the conceptual design report. Much of the geological-hydrological information was gathered in the Lyons area. This report is organized in 16 sections: considerations leading to the proposed Repository, design requirements and criteria, a description of the Lyons site and its environs, land improvements, support facilities, utilities, different impacts of Repository operations, safety analysis, design confirmation program, operational management, requirements for eventually decommissioning the facility, design criteria for protection from severe natural events, and the proposed program of experimental investigations. (DLC)

  11. Preliminary Cluster Analysis For Several Representatives Of Genus Kerivoula (Chiroptera: Vespertilionidae) in Borneo

    Science.gov (United States)

    Hasan, Noor Haliza; Abdullah, M. T.

    2008-01-01

    The aim of the study is to use cluster analysis on morphometric parameters within the genus Kerivoula to produce a dendrogram and to determine the suitability of this method to describe the relationship among species within this genus. A total of 15 adult male individuals from genus Kerivoula taken from sampling trips around Borneo and specimens kept at the zoological museum of Universiti Malaysia Sarawak were examined. A total of 27 characters using dental, skull and external body measurements were recorded. Clustering analysis illustrated the grouping and morphometric relationships between the species of this genus. It has clearly separated each species from each other despite the overlapping of measurements of some species within the genus. Cluster analysis provides an alternative approach to make a preliminary identification of a species.

  12. Kinetic roughening transition and missing regime transition of melt crystallized polybutene-1 tetragonal phase: growth kinetics analysis

    Institute of Scientific and Technical Information of China (English)

    Motoi YAMASHITA

    2009-01-01

    The morphology and lateral growth rate of isotactic polybutene-1 (it-PBl) have been investigated for crystallization from the melt over a wide range of crystallization temperatures from 50 to 110°C. The morphology of it-PBl crystals is a rounded shape at crystallization temperatures lower than 85°C, while lamellar single crystals possess faceted morphology at higher crystallization temperatures. The kinetic roughening transition occurs around 85°C. The nucleation and growth mechanism for crystallization does not work below 85°C, since the growth face is rough. However, the growth rate shows the supercooling dependence derived from the nucleation and growth mechanism. The nucleation theory seems still to work even for rough surface growth. Possible mechanisms for the crystal growth of this polymer are discussed.

  13. CHEMKIN-III: A FORTRAN chemical kinetics package for the analysis of gas-phase chemical and plasma kinetics

    Energy Technology Data Exchange (ETDEWEB)

    Kee, R.J.; Rupley, F.M.; Meeks, E.; Miller, J.A.

    1996-05-01

    This document is the user`s manual for the third-generation CHEMKIN package. CHEMKIN is a software package whose purpose is to facilitate the formation, solution, and interpretation of problems involving elementary gas-phase chemical kinetics. It provides a flexible and powerful tool for incorporating complex chemical kinetics into simulations of fluid dynamics. The package consists of two major software components: an Interpreter and a Gas-Phase Subroutine Library. The Interpreter is a program that reads a symbolic description of an elementary, user-specified chemical reaction mechanism. One output from the Interpreter is a data file that forms a link to the Gas-Phase Subroutine Library. This library is a collection of about 100 highly modular FORTRAN subroutines that may be called to return information on equations of state, thermodynamic properties, and chemical production rates. CHEMKIN-III includes capabilities for treating multi-fluid plasma systems, that are not in thermal equilibrium. These new capabilities allow researchers to describe chemistry systems that are characterized by more than one temperature, in which reactions may depend on temperatures associated with different species; i.e. reactions may be driven by collisions with electrons, ions, or charge-neutral species. These new features have been implemented in such a way as to require little or no changes to CHEMKIN implementation for systems in thermal equilibrium, where all species share the same gas temperature. CHEMKIN-III now has the capability to handle weakly ionized plasma chemistry, especially for application related to advanced semiconductor processing.

  14. An Inducible Reconstitution System for the Real-Time Kinetic Analysis of Protease Activity and Inhibition Inside the Membrane.

    Science.gov (United States)

    Baker, R P; Urban, S

    2017-01-01

    Intramembrane proteases are an ancient and diverse group of multispanning membrane proteins that cleave transmembrane substrates inside the membrane to effect a wide range of biological processes. As proteases, a clear understanding of their function requires kinetic dissection of their catalytic mechanism, but this is difficult to achieve for membrane proteins. Kinetic measurements in detergent systems are complicated by micelle fusion/exchange, which introduces an additional kinetic step and imposes system-specific behaviors (e.g., cooperativity). Conversely, kinetic analysis in proteoliposomes is hindered by premature substrate cleavage during coreconstitution, and lack of methods to quantify proteolysis in membranes in real time. In this chapter, we describe a method for the real-time kinetic analysis of intramembrane proteolysis in model liposomes. Our assay is inducible, because the enzyme is held inactive by low pH during reconstitution, and fluorogenic, since fluorescence emission from the substrate is quenched near lipids but restored upon proteolytic release from the membrane. The precise measurement of initial reaction velocities continuously in real time facilitates accurate steady-state kinetic analysis of intramembrane proteolysis and its inhibition inside the membrane environment. Using real data we describe a step-by-step strategy to implement this assay for essentially any intramembrane protease.

  15. Kinetic and Kinematic Analysis for Assessing the Differences in Countermovement Jump Performance in Rugby Players.

    Science.gov (United States)

    Floría, Pablo; Gómez-Landero, Luis A; Suárez-Arrones, Luis; Harrison, Andrew J

    2016-09-01

    Floría, P, Gómez-Landero, LA, Suárez-Arrones, L, and Harrison, AJ. Kinetic and kinematic analysis for assessing the differences in countermovement jump performance in rugby players. J Strength Cond Res 30(9): 2533-2539, 2016-The aim of this study was to ascertain the differences in kinetic and kinematic profiles between better and poorer performers of the vertical jump within a homogeneous group of trained adults. Fifty rugby players were divided into low scoring (LOW) and high scoring (HIGH) groups based on their performance in the vertical jump. The force, velocity, displacement, and rate of force development (RFD)-time curves were analyzed to determine the differences between groups. The analysis of the data showed differences in all the patterns of the ensemble mean curves of the HIGH and LOW groups. During the eccentric phase, the differences in the HIGH group with respect to the LOW group were lower crouch position, higher downward velocity, and higher force and RFD during the braking of the downward movement. During the concentric phase, the HIGH group achieved higher upward velocity, higher force at the end of phase, and a higher position at takeoff. The higher jump performances seem to be related to a more effective stretch-shortening cycle function that is characterized by a deeper and faster countermovement with higher eccentric forces being applied to decelerate the downward movement leading to enhanced force generation during the concentric phase.

  16. Kinetic Model of LiFePO4 Formation Using Non-Isothermal Thermogravimetric Analysis

    Directory of Open Access Journals (Sweden)

    Abdul Halim

    2014-03-01

    Full Text Available The formation reaction of LiFePO4 from decomposition of precursors LiOH, FeSO4.7H2O and (NH42HPO4 with mol ratio of Li:Fe:P=1:1:1 was investigated. The experiment was carried out by thermogravimetric differential thermal analysis (TG-DTA method using nitrogen as atmosfer at a constant heating rate to obtain kinetic constant parameters. Several heating rates were selected, there are 5, 7, 10, 15, 17.5, 22.5 and 25 °C/min. Activation energy, pre-exponential factor and reaction order were taken using Kissinger method and obtained respectively 56.086 kJ/mol, 6.95×108 min-1, and 1.058. Based on fitting result between reaction model and experiment were obtained that reaction obeyed the three dimension diffusion model. © 2014 BCREC UNDIP. All rights reservedReceived: 19th September 2013; Revised: 9th December 2013; Accepted: 23rd January 2014 [How to Cite: Halim, A., Widiyastuti, W., Setyawan, H., Winardi, S. (2014. Kinetic of LiFePO4 For-mation Using Non-isothermal Thermogravimetric Analysis. Bulletin of Chemical Reaction Engineering & Catalysis, 9 (1: 60-65. (doi:10.9767/bcrec.9.1.5508.60-65][Permalink/DOI: http://dx.doi.org/10.9767/bcrec.9.1.5508.60-65] 

  17. The purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Clostridium botulinum

    Energy Technology Data Exchange (ETDEWEB)

    Dobson, Renwick C. J., E-mail: rdobson@unimelb.edu.au; Atkinson, Sarah C. [Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria 3010 (Australia); Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, University of Melbourne, Parkville, Victoria 3010 (Australia); Gorman, Michael A. [St Vincents Institute, 9 Princes Street, Fitzroy, Victoria 3065 (Australia); Newman, Janet M. [CSIRO Division of Molecular and Health Technologies, 343 Royal Parade, Parkville, Victoria 3052 (Australia); Parker, Michael W. [Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria 3010 (Australia); Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, University of Melbourne, Parkville, Victoria 3010 (Australia); St Vincents Institute, 9 Princes Street, Fitzroy, Victoria 3065 (Australia); Perugini, Matthew A. [Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria 3010 (Australia); Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Road, University of Melbourne, Parkville, Victoria 3010 (Australia)

    2008-03-01

    Dihydrodipicolinate synthase (DHDPS), an enzyme in the lysine-biosynthetic pathway, is a promising target for antibiotic development against pathogenic bacteria. Here, the expression, purification, crystallization and preliminary diffraction analysis of DHDPS from C. botulinum are reported. In recent years, dihydrodipicolinate synthase (DHDPS; EC 4.2.1.52) has received considerable attention from both mechanistic and structural viewpoints. This enzyme, which is part of the diaminopimelate pathway leading to lysine, couples (S)-aspartate-β-semialdehyde with pyruvate via a Schiff base to a conserved active-site lysine. In this paper, the expression, purification, crystallization and preliminary X-ray diffraction analysis of DHDPS from Clostridium botulinum, an important bacterial pathogen, are presented. The enzyme was crystallized in a number of forms, predominantly using PEG precipitants, with the best crystal diffracting to beyond 1.9 Å resolution and displaying P4{sub 2}2{sub 1}2 symmetry. The unit-cell parameters were a = b = 92.9, c = 60.4 Å. The crystal volume per protein weight (V{sub M}) was 2.07 Å{sup 3} Da{sup −1}, with an estimated solvent content of 41%. The structure of the enzyme will help guide the design of novel therapeutics against the C. botulinum pathogen.

  18. A Preliminary Analysis of Reactor Performance Test (LOEP) for a Research Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyeonil; Park, Su-Ki [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    The final phase of commissioning is reactor performance test, which is to prove the integrated performance and safety of the research reactor at full power with fuel loaded such as neutron power calibration, Control Absorber Rod/Second Shutdown Rod drop time, InC function test, Criticality, Rod worth, Core heat removal with natural mechanism, and so forth. The last test will be safety-related one to assure the result of the safety analysis of the research reactor is marginal enough to be sure about the nuclear safety by showing the reactor satisfies the acceptance criteria of the safety functions such as for reactivity control, maintenance of auxiliaries, reactor pool water inventory control, core heat removal, and confinement isolation. After all, the fuel integrity will be ensured by verifying there is no meaningful change in the radiation levels. To confirm the performance of safety equipment, loss of normal electric power (LOEP), possibly categorized as Anticipated Operational Occurrence (AOO), is selected as a key experiment to figure out how safe the research reactor is before turning over the research reactor to the owner. This paper presents a preliminary analysis of the reactor performance test (LOEP) for a research reactor. The results showed how different the transient between conservative estimate and best estimate will look. Preliminary analyses have shown all probable thermal-hydraulic transient behavior of importance as to opening of flap valve, minimum critical heat flux ratio, the change of flow direction, and important values of thermal-hydraulic parameters.

  19. Engineering Substrate Preference in Subtilisin: Structural and Kinetic Analysis of a Specificity Mutant

    Energy Technology Data Exchange (ETDEWEB)

    Ruan, Biao; London, Viktoriya; Fisher, Kathryn E.; Gallagher, D. Travis; Bryan, Philip N. (UMBI)

    2008-08-06

    Bacillus subtilisin has been a popular model protein for engineering altered substrate specificity. Although some studies have succeeded in increasing the specificity of subtilisin, they also demonstrate that high specificity is difficult to achieve solely by engineering selective substrate binding. In this paper, we analyze the structure and transient state kinetic behavior of Sbt160, a subtilisin engineered to strongly prefer substrates with phenylalanine or tyrosine at the P4 position. As in previous studies, we measure improvements in substrate affinity and overall specificity. Structural analysis of an inactive version of Sbt160 in complex with its cognate substrate reveals improved interactions at the S4 subsite with a P4 tyrosine. Comparison of transient state kinetic behavior against an optimal sequence (DFKAM) and a similar, but suboptimal, sequence (DVRAF) reveals the kinetic and thermodynamic basis for increased specificity, as well as the limitations of this approach. While highly selective substrate binding is achieved in Sbt160, several factors cause sequence specificity to fall short of that observed with natural processing subtilisins. First, for substrate sequences which are nearly optimal, the acylation reaction becomes faster than substrate dissociation. As a result, the level of discrimination among these substrates diminishes due to the coupling between substrate binding and the first chemical step (acylation). Second, although Sbt160 has 24-fold higher substrate affinity for the optimal substrate DFKAM than for DVRAF, the increased substrate binding energy is not translated into improved transition state stabilization of the acylation reaction. Finally, as interactions at subsites become stronger, the rate-determining step in peptide hydrolysis changes from acylation to product release. Thus, the release of the product becomes sluggish and leads to a low k{sub cat} for the reaction. This also leads to strong product inhibition of substrate

  20. Pyrolysis characteristics and kinetics of microalgae via thermogravimetric analysis (TGA): A state-of-the-art review.

    Science.gov (United States)

    Bach, Quang-Vu; Chen, Wei-Hsin

    2017-06-19

    Pyrolysis is a promising route for biofuels production from microalgae at moderate temperatures (400-600°C) in an inert atmosphere. Depending on the operating conditions, pyrolysis can produce biochar and/or bio-oil. In practice, knowledge for thermal decomposition characteristics and kinetics of microalgae during pyrolysis is essential for pyrolyzer design and pyrolysis optimization. Recently, the pyrolysis kinetics of microalgae has become a crucial topic and received increasing interest from researchers. Thermogravimetric analysis (TGA) has been employed as a proven technique for studying microalgae pyrolysis in a kinetic control regime. In addition, a number of kinetic models have been applied to process the TGA data for kinetic evaluation and parameters estimation. This paper aims to provide a state-of-the art review on recent research activities in pyrolysis characteristics and kinetics of various microalgae. Common kinetic models predicting the thermal degradation of microalgae are examined and their pros and cons are illustrated. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Preliminary design and thermal analysis of device for finish cooling Jaffa biscuits in a.d. 'Jaffa'- Crvenka

    Directory of Open Access Journals (Sweden)

    Salemović Duško R.

    2015-01-01

    Full Text Available In this paper preliminary design of device for finish cooling chocolate topping of biscuits in A.D. 'Jaffa'- Crvenka was done. The proposed preliminary design followed by the required technological process of finish cooling biscuits and required parameters of process which was supposed to get and which represented part of project task. Thermal analysis was made and obtained percentage error between surface contact of the air and chocolate topping, obtained from heat balance and geometrical over proposed preliminary design, wasn't more than 0.67%. This is a preliminary design completely justified because using required length of belt conveyor receive required temperature of chocolate topping at the end of the cooling process.

  2. Establishment of steady-state metabolism of ethanol in perfused rat liver: the quantitative analysis using kinetic mechanism-based rate equations of alcohol dehydrogenase.

    Science.gov (United States)

    Yao, Chung-Tay; Lai, Ching-Long; Hsieh, Hsiu-Shan; Chi, Chin-Wen; Yin, Shih-Jiun

    2010-09-01

    Alcohol dehydrogenase (ADH) catalyzes oxidation of ingested ethanol to acetaldehyde, the first step in hepatic metabolism. The purpose of this study was to establish an ex vivo rat liver perfusion system under defined and verified steady states with respect to the metabolites and the metabolic rates, and to quantitatively correlate the observed rates with simulations based on the kinetic mechanism-based rate equations of rat liver ADH. Class I ADH1 was isolated from male Sprague-Dawley rats and characterized by steady-state kinetics in the Krebs-Ringer perfusion buffer with supplements. Nonrecirculating liver perfusion with constant input of ethanol at near physiological hepatic blood flow rate was performed in situ. Ethanol and the related metabolites acetaldehyde, acetate, lactate, and pyruvate in perfusates were determined. Results of the initial velocity, product, and dead-end inhibition studies showed that rat ADH1 conformed to the Theorell-Chance Ordered Bi Bi mechanism. Steady-state metabolism of ethanol in the perfused liver maintained up to 3h as evidenced by the steady-state levels of ethanol and metabolites in the effluent, and the steady-state ethanol disappearance rates and acetate production rates. The changes of the metabolic rates were qualitatively and in general quantitatively correlated to the results from simulations with the kinetic rate equations of ADH1 under a wide range of ethanol, in the presence of competitive inhibitor 4-methylpyrazole and of uncompetitive inhibitor isobutyramide. Preliminary flux control analysis estimated that apparent C(ADH)(J) in the perfused liver may approximate 0.7 at constant infusion with 1-2 mM ethanol, suggesting that ADH plays a major but not the exclusive role in governing hepatic ethanol metabolism. The reported steady-state rat liver perfusion system may potentially be applicable to other drug or drug-ethanol interaction studies.

  3. Mini-DIAL system measurements coupled with multivariate data analysis to identify TIC and TIM simulants: preliminary absorption database analysis.

    Science.gov (United States)

    Gaudio, P.; Malizia, A.; Gelfusa, M.; Martinelli, E.; Di Natale, C.; Poggi, L. A.; Bellecci, C.

    2017-01-01

    Nowadays Toxic Industrial Components (TICs) and Toxic Industrial Materials (TIMs) are one of the most dangerous and diffuse vehicle of contamination in urban and industrial areas. The academic world together with the industrial and military one are working on innovative solutions to monitor the diffusion in atmosphere of such pollutants. In this phase the most common commercial sensors are based on “point detection” technology but it is clear that such instruments cannot satisfy the needs of the smart cities. The new challenge is developing stand-off systems to continuously monitor the atmosphere. Quantum Electronics and Plasma Physics (QEP) research group has a long experience in laser system development and has built two demonstrators based on DIAL (Differential Absorption of Light) technology could be able to identify chemical agents in atmosphere. In this work the authors will present one of those DIAL system, the miniaturized one, together with the preliminary results of an experimental campaign conducted on TICs and TIMs simulants in cell with aim of use the absorption database for the further atmospheric an analysis using the same DIAL system. The experimental results are analysed with standard multivariate data analysis technique as Principal Component Analysis (PCA) to develop a classification model aimed at identifying organic chemical compound in atmosphere. The preliminary results of absorption coefficients of some chemical compound are shown together pre PCA analysis.

  4. Accounting for the kinetics in order parameter analysis: lessons from theoretical models and a disordered peptide

    CERN Document Server

    Berezovska, Ganna; Mostarda, Stefano; Rao, Francesco

    2012-01-01

    Molecular simulations as well as single molecule experiments have been widely analyzed in terms order parameters, the latter representing candidate probes for the relevant degrees of freedom. Notwithstanding this approach is very intuitive, mounting evidence showed that such description is not accurate, leading to ambiguous definitions of states and wrong kinetics. To overcome these limitations a framework making use of order parameter fluctuations in conjunction with complex network analysis is investigated. Derived from recent advances in the analysis of single molecule time traces, this approach takes into account of the fluctuations around each time point to distinguish between states that have similar values of the order parameter but different dynamics. Snapshots with similar fluctuations are used as nodes of a transition network, the clusterization of which into states provides accurate Markov-State-Models of the system under study. Application of the methodology to theoretical models with a noisy orde...

  5. Kinetic analysis of photocatalytic oxidation of gas-phase formaldehyde over titanium dioxide.

    Science.gov (United States)

    Liu, Hongmin; Lian, Zhiwei; Ye, Xiaojiang; Shangguan, Wenfeng

    2005-07-01

    Degradation of formaldehyde with different initial concentration over titanium dioxide was carried out in a photocatalytic reactor. Photocatalytic rates were well described by the simplified Langmuir-Hinshelwood model. The kinetic analysis shows that the apparent first-order reaction coefficient is lower and half-life of photocatalysis is longer for low concentration than for high concentration formaldehyde. A network formation model of the photocatalytic products was established. Experimental results and analysis demonstrate that carbon dioxide concentration and carbon monoxide concentration in gas phase vary exponentially with the illumination time and may be even higher than gas-phase formaldehyde concentration if there is much pre-adsorbed formaldehyde in adsorption equilibrium on catalysts before illumination. Carbon monoxide is found to be one of the by-products during formaldehyde photooxidation.

  6. Sensitivity analysis of intracellular signaling pathway kinetics predicts targets for stem cell fate control.

    Directory of Open Access Journals (Sweden)

    Alborz Mahdavi

    2007-07-01

    Full Text Available Directing stem cell fate requires knowledge of how signaling networks integrate temporally and spatially segregated stimuli. We developed and validated a computational model of signal transducer and activator of transcription-3 (Stat3 pathway kinetics, a signaling network involved in embryonic stem cell (ESC self-renewal. Our analysis identified novel pathway responses; for example, overexpression of the receptor glycoprotein-130 results in reduced pathway activation and increased ESC differentiation. We used a systematic in silico screen to identify novel targets and protein interactions involved in Stat3 activation. Our analysis demonstrates that signaling activation and desensitization (the inability to respond to ligand restimulation is regulated by balancing the activation state of a distributed set of parameters including nuclear export of Stat3, nuclear phosphatase activity, inhibition by suppressor of cytokine signaling, and receptor trafficking. This knowledge was used to devise a temporally modulated ligand delivery strategy that maximizes signaling activation and leads to enhanced ESC self-renewal.

  7. An equivalent finite element method to kinetics analysis of complex mechanism

    Institute of Scientific and Technical Information of China (English)

    CHE Ren-wei; LU Nian-li

    2005-01-01

    The Finite Element Method was combined with the results from considerable analysis, producing a new kinetics analysis method of EFEM for a mechanism in truss, geared system, and assembled system. The equivalent principle and the motive differential equation of the system were derived by using an equivalent element, a virtual inertia matrix, and a systematic force matrix. The element' s mass matrix expression in the two dimensional and three dimensional mechanisms of the equivalent element was determined. The equivalent mass matrixes fashion of the Jacobin matrix, generalized coordinate matrix, and equivalent forces matrix were also determined. It was validated by two examples that the new method was normal, simple and direct, and had a higher efficiency than alternative methods; this is regardless of whether traditional methods are used with differential equations and calculated by using a computer.

  8. Kinetic and kinematic analysis of gait pattern of 13 year old children with unilateral genu valgum.

    Science.gov (United States)

    Ganesan, B; Fong, K N K; Luximon, A; Al-Jumaily, A

    2016-07-01

    Genu valgum is a common knee deformity in growing children. It alters the alignment of the lower extremity, body posture, and gait pattern of the children. Understanding of kinematic and kinetic parameters of gait in genu valgum is essential for planning and implementing the intervention to correcting the valgus deformity. The aim of this paper is to investigate the kinetic and kinematic gait differences in children with genu valgum. A 13-year old girl with left side unilateral genu valgum and a closely matched healthy counterpart were recruited to compare the kinetic and kinematic parameters of their gait performances, and they were captured by The VICON motion analysis system. The results showed that the child with genu valgum had lower left and right knee angles (39.6˚; 30.2˚) and higher ankle angles (35.6˚; 28.4˚) than the healthy subject (64.2˚, 60.2˚). In addition, the child with genu valgum had lower moments on the left side of the knee (42.1 mm.N) than unaffected right knee (73.9 mm.N). Also, the ground reaction force was (0.7 N) lower in the affected knee of the child with genu valgum than the normal subject. This study revealed that there were decreased knee and ankle moments and lower knee and ankle ground reaction forces in the affected genu valgum extremity when compared with the healthy counterpart. These changes might be responsible for the altering gait pattern of the child with genu valgum.

  9. Multijoint kinetic chain analysis of knee extension during the soccer instep kick.

    Science.gov (United States)

    Naito, Kozo; Fukui, Yosuke; Maruyama, Takeo

    2010-04-01

    Although previous studies have shown that motion-dependent interactions between adjacent segments play an important role in producing knee extension during the soccer instep kick, detailed knowledge about the mechanisms underlying those interactions is lacking. The present study aimed to develop a 3-D dynamical model for the multijoint kinetic chain of the instep kick in order to quantify the contributions of the causal dynamical factors to the production of maximum angular velocity during knee extension. Nine collegiate soccer players volunteered to participate in the experiment and performed instep kicking movements while 3-D positional data and the ground reaction force were measured. A dynamical model was developed in the form of a linked system containing 8 segments and 18 joint rotations, and the knee extension/flexion motion was decomposed into causal factors related to muscular moment, gyroscopic moment, centrifugal force, Coriolis force, gravity, proximal endpoint linear acceleration, and external force-dependent terms. The rapid knee extension during instep kicking was found to result almost entirely from kicking leg centrifugal force, trunk rotation muscular moment, kicking leg Coriolis force, and trunk rotation gyroscopic-dependent components. Based on the finding that rapid knee extension during instep kicking stems from multiple dynamical factors, it is suggested that the multijoint kinetic chain analysis used in the present study is more useful for achieving a detailed understanding of the cause of rapid kicking leg movement than the previously used 2-D, two-segment kinetic chain model. The present results also indicated that the centrifugal effect due to the kicking hip flexion angular velocity contributed substantially to the generation of a rapid knee extension, suggesting that the adjustment between the kicking hip flexion angular velocity and the leg configuration (knee flexion angle) is more important for effective instep kicking than other

  10. Goal-oriented sensitivity analysis for lattice kinetic Monte Carlo simulations.

    Science.gov (United States)

    Arampatzis, Georgios; Katsoulakis, Markos A

    2014-03-28

    In this paper we propose a new class of coupling methods for the sensitivity analysis of high dimensional stochastic systems and in particular for lattice Kinetic Monte Carlo (KMC). Sensitivity analysis for stochastic systems is typically based on approximating continuous derivatives with respect to model parameters by the mean value of samples from a finite difference scheme. Instead of using independent samples the proposed algorithm reduces the variance of the estimator by developing a strongly correlated-"coupled"- stochastic process for both the perturbed and unperturbed stochastic processes, defined in a common state space. The novelty of our construction is that the new coupled process depends on the targeted observables, e.g., coverage, Hamiltonian, spatial correlations, surface roughness, etc., hence we refer to the proposed method as goal-oriented sensitivity analysis. In particular, the rates of the coupled Continuous Time Markov Chain are obtained as solutions to a goal-oriented optimization problem, depending on the observable of interest, by considering the minimization functional of the corresponding variance. We show that this functional can be used as a diagnostic tool for the design and evaluation of different classes of couplings. Furthermore, the resulting KMC sensitivity algorithm has an easy implementation that is based on the Bortz-Kalos-Lebowitz algorithm's philosophy, where events are divided in classes depending on level sets of the observable of interest. Finally, we demonstrate in several examples including adsorption, desorption, and diffusion Kinetic Monte Carlo that for the same confidence interval and observable, the proposed goal-oriented algorithm can be two orders of magnitude faster than existing coupling algorithms for spatial KMC such as the Common Random Number approach. We also provide a complete implementation of the proposed sensitivity analysis algorithms, including various spatial KMC examples, in a supplementary MATLAB

  11. Goal-oriented sensitivity analysis for lattice kinetic Monte Carlo simulations

    Science.gov (United States)

    Arampatzis, Georgios; Katsoulakis, Markos A.

    2014-03-01

    In this paper we propose a new class of coupling methods for the sensitivity analysis of high dimensional stochastic systems and in particular for lattice Kinetic Monte Carlo (KMC). Sensitivity analysis for stochastic systems is typically based on approximating continuous derivatives with respect to model parameters by the mean value of samples from a finite difference scheme. Instead of using independent samples the proposed algorithm reduces the variance of the estimator by developing a strongly correlated-"coupled"- stochastic process for both the perturbed and unperturbed stochastic processes, defined in a common state space. The novelty of our construction is that the new coupled process depends on the targeted observables, e.g., coverage, Hamiltonian, spatial correlations, surface roughness, etc., hence we refer to the proposed method as goal-oriented sensitivity analysis. In particular, the rates of the coupled Continuous Time Markov Chain are obtained as solutions to a goal-oriented optimization problem, depending on the observable of interest, by considering the minimization functional of the corresponding variance. We show that this functional can be used as a diagnostic tool for the design and evaluation of different classes of couplings. Furthermore, the resulting KMC sensitivity algorithm has an easy implementation that is based on the Bortz-Kalos-Lebowitz algorithm's philosophy, where events are divided in classes depending on level sets of the observable of interest. Finally, we demonstrate in several examples including adsorption, desorption, and diffusion Kinetic Monte Carlo that for the same confidence interval and observable, the proposed goal-oriented algorithm can be two orders of magnitude faster than existing coupling algorithms for spatial KMC such as the Common Random Number approach. We also provide a complete implementation of the proposed sensitivity analysis algorithms, including various spatial KMC examples, in a supplementary MATLAB

  12. Preliminary Nuclear Analysis for the HANARO Fuel Element with Burnable Absorber

    Energy Technology Data Exchange (ETDEWEB)

    Seo, Chul Gyo; Kim, So Young; In, Won Ho [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    Burnable absorber is used for reducing reactivity swing and power peaking in high performance research reactors. Development of the HANARO fuel element with burnable absorber was started in the U-Mo fuel development program at HANARO, but detailed full core analysis was not performed because the current HANARO fuel management system is uncertain to analysis the HANARO core with burnable absorber. A sophisticated reactor physics system is required to analysis the core. The McCARD code was selected and the detailed McCARD core models, in which the basic HANARO core model was developed by one of the McCARD developers, are used in this study. The development of nuclear fuel requires a long time and correct developing direction especially by the nuclear analysis. This paper presents a preliminary nuclear analysis to promote the fuel development. Based on the developed fuel, the further nuclear analysis will improve reactor performance and safety. Basic nuclear analysis for the HANARO and the AHR were performed for getting the proper fuel elements with burnable absorber. Addition of 0.3 - 0.4% Cd to the fuel meat is promising for the current HANARO fuel element. Small addition of burnable absorber may not change any fuel characteristics of the HANARO fuel element, but various basic tests and irradiation tests at the HANARO core are required.

  13. Treatment by gliding arc of epoxy resin: preliminary analysis of surface modifications

    Science.gov (United States)

    Faubert, F.; Wartel, M.; Pellerin, N.; Pellerin, S.; Cochet, V.; Regnier, E.; Hnatiuc, B.

    2016-12-01

    Treatments with atmospheric pressure non-thermal plasma are easy to implement and inexpensive. Among them gliding arc (GlidArc) remains rarely used in surface treatment of polymers. However, it offers economic and flexible way to treat quickly large areas. In addition the choice of carrier gas makes it possible to bring the active species and other radicals allowing different types of grafting and functionalization of the treated surfaces, for example in order to apply for anti-biofouling prevention. This preliminary work includes analysis of the surface of epoxy resins by infrared spectroscopy: the different affected chemical bonds were studied depending on the duration of treatment. The degree of oxidation (the C/O ratio) is obtained by X-ray microanalysis and contact angle analysis have been performed to determinate the wettability properties of the treated surface. A spectroscopic study of the plasma allows to determine the possible active species in the different zones of the discharge.

  14. Los Alamos National Laboratory corregated metal pipe saw facility preliminary safety analysis report. Volume I

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1990-09-19

    This Preliminary Safety Analysis Report addresses site assessment, facility design and construction, and design operation of the processing systems in the Corrugated Metal Pipe Saw Facility with respect to normal and abnormal conditions. Potential hazards are identified, credible accidents relative to the operation of the facility and the process systems are analyzed, and the consequences of postulated accidents are presented. The risk associated with normal operations, abnormal operations, and natural phenomena are analyzed. The accident analysis presented shows that the impact of the facility will be acceptable for all foreseeable normal and abnormal conditions of operation. Specifically, under normal conditions the facility will have impacts within the limits posted by applicable DOE guidelines, and in accident conditions the facility will similarly meet or exceed the requirements of all applicable standards. 16 figs., 6 tabs.

  15. Preliminary Uncertainty Analysis for SMART Digital Core Protection and Monitoring System

    Energy Technology Data Exchange (ETDEWEB)

    Koo, Bon Seung; In, Wang Kee; Hwang, Dae Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2012-05-15

    The Korea Atomic Energy Research Institute (KAERI) developed on-line digital core protection and monitoring systems, called SCOPS and SCOMS as a part of SMART plant protection and monitoring system. SCOPS simplified the protection system by directly connecting the four RSPT signals to each core protection channel and eliminated the control element assembly calculator (CEAC) hardware. SCOMS adopted DPCM3D method in synthesizing core power distribution instead of Fourier expansion method being used in conventional PWRs. The DPCM3D method produces a synthetic 3-D power distribution by coupling a neutronics code and measured in-core detector signals. The overall uncertainty analysis methodology which is used statistically combining uncertainty components of SMART core protection and monitoring system was developed. In this paper, preliminary overall uncertainty factors for SCOPS/SCOMS of SMART initial core were evaluated by applying newly developed uncertainty analysis method

  16. Preliminary cladistic analysis of genera of the cestode order Trypanorhyncha Diesing, 1863.

    Science.gov (United States)

    Beveridge, I; Campbell, R A; Palm, H W

    1999-01-01

    A preliminary cladistic analysis was carried out on the 49 currently recognised genera of the order Trypanorhyncha. Forty-four characters were analysed; a functional outgroup was used for scolex and strobilar characters, while Nybelinia was utilised to polarise characters related to the rhyncheal system. Eight well-resolved clades were evident in the resultant cladogram, which is compared with existing phenetic classifications. An analysis of families resulted in a similar clustering of taxa to that observed in the case of the genera. The results suggest that two key characters used in existing classifications, namely the presence of sensory fossettes on the bothridia and the development of atypical heteroacanth and poeciloacanth armatures from typical heteroacanth armatures, have occurred on several occasions. Some clades provide support for the arrangements used in current classifications. Suggestions are made for future avenues of research which might provide more robust phylogenetic data for the Trypanorhyncha.

  17. Preliminary Evaluation of MapReduce for High-Performance Climate Data Analysis

    Science.gov (United States)

    Duffy, Daniel Q.; Schnase, John L.; Thompson, John H.; Freeman, Shawn M.; Clune, Thomas L.

    2012-01-01

    MapReduce is an approach to high-performance analytics that may be useful to data intensive problems in climate research. It offers an analysis paradigm that uses clusters of computers and combines distributed storage of large data sets with parallel computation. We are particularly interested in the potential of MapReduce to speed up basic operations common to a wide range of analyses. In order to evaluate this potential, we are prototyping a series of canonical MapReduce operations over a test suite of observational and climate simulation datasets. Our initial focus has been on averaging operations over arbitrary spatial and temporal extents within Modern Era Retrospective- Analysis for Research and Applications (MERRA) data. Preliminary results suggest this approach can improve efficiencies within data intensive analytic workflows.

  18. Dimensions of Human-Work Domain Interaction: A Preliminary Analysis for the Design of a Corporate Digital Library.

    Science.gov (United States)

    Xie, Hong

    2003-01-01

    Applies the cognitive system engineering approach to investigate human-work interaction at a corporate setting. Reports preliminary analysis of data collected from diary analysis and interview of 20 subjects. Results identify three dimensions for each of four interactive activities involved in human-work interaction and their relationships.…

  19. Oxidation kinetic analysis of a mixed uranium dicarbide and graphite compound

    Energy Technology Data Exchange (ETDEWEB)

    Marchand, M., E-mail: mickael.marchand@cea.fr [Commissariat à l’Energie Atomique et aux énergies alternatives, CEA, CADARACHE, DEN, DEC, SPUA/Laboratoire des Combustibles Uranium, 13108 Saint Paul-lez-Durance Cedex (France); Fiquet, O., E-mail: olivier.fiquet@cea.fr [Commissariat à l’Energie Atomique et aux énergies alternatives, CEA, CADARACHE, DEN, DEC, SPUA/Laboratoire des Combustibles Uranium, 13108 Saint Paul-lez-Durance Cedex (France); Brothier, M. [Commissariat à l’Energie Atomique et aux énergies alternatives, CEA, CADARACHE, DEN, DEC, SPUA/Laboratoire des Combustibles Uranium, 13108 Saint Paul-lez-Durance Cedex (France)

    2013-06-15

    Highlights: ► Experimental study of uranium carbides and graphite powder oxidations. ► Single rate limiting step identification by extensive kinetic analysis. ► Pseudo-steady-state validation during chemical conversion. ► Combination of TGA, TDA, XRD and gas phase chromatography results. -- Abstract: The oxidation of a mixed uranium dicarbide and graphite powder has been investigated by simultaneous thermal gravimetric (TGA) and differential thermal (DTA) analyses coupled with gas phase chromatography. For isothermal oxidation conditions with temperatures below 330 °C, only the UC{sub 2} chemical phase is progressively oxidised into U{sub 3}O{sub 8} oxides. Parabolic weight gain curves as a function of oxidation over time were obtained. A detailed kinetic study is proposed to establish a pseudo-steady-state during the oxidation process. Using an experimental method based on the sudden temperature increases, a single rate-limiting step has been validated and then modelled by a 3D diffusion law. An apparent activation energy calculated from the Arrhenius representation has been evaluated at −35 kJ/mol, thus describing the diffusion of oxygen through the oxide layer.

  20. Pyrolysis kinetics of raw and hydrothermally carbonized Karanj (Pongamia pinnata) fruit hulls via thermogravimetric analysis.

    Science.gov (United States)

    Islam, Md Azharul; Asif, M; Hameed, B H

    2015-03-01

    The pyrolysis of karanj fruit hulls (KFH) and karanj fruit hull hydrothermal carbonization (KFH-HTC) hydrochar was thermogravimetrically investigated under a nitrogen environment at 5 °C/min, 10 °C/min, and 20 °C/min. The pyrolysis decomposition of KFH biomass was faster than that of KFH-HTC hydrochar because of the high volatility and fixed carbon of KFH biomass. Weight loss percentage was also affected by the heating rates. The kinetic data were evaluated with the Kissinger-Akahira-Sunose and Flynn-Wall-Ozawa methods. The activation energy values obtained with these two methods were 61.06 and 68.53 kJ/mol for KFH biomass and 130.49 and 135.87 kJ/mol for KFH-HTC hydrochar, respectively. The analysis of kinetic process mechanisms was verified with the Coats-Redfern method. KFH-HTC hydrochar may play a potential role in transforming biomass to energy-rich feedstock for thermochemical applications because of its high heating value, high fixed carbon, and low ash and sulfur contents.

  1. Microwave treatment of dairy manure for resource recovery: Reaction kinetics and energy analysis.

    Science.gov (United States)

    Srinivasan, Asha; Liao, Ping H; Lo, Kwang V

    2016-12-01

    A newly designed continuous-flow 915 MHz microwave wastewater treatment system was used to demonstrate the effectiveness of the microwave enhanced advanced oxidation process (MW/H2O2-AOP) for treating dairy manure. After the treatment, about 84% of total phosphorus and 45% of total chemical oxygen demand were solubilized with the highest H2O2 dosage (0.4% H2O2 per %TS). The reaction kinetics of soluble chemical oxygen demand revealed activation energy to be in the range of 5-22 kJ mole(-1). The energy required by the processes was approximately 0.16 kWh per liter of dairy manure heated. A higher H2O2 dosage used in the system had a better process performance in terms of solids solubilization, reaction kinetics, and energy consumption. Cost-benefit analysis for a farm-scale MW/H2O2-AOP treatment system was also presented. The results obtained from this study would provide the basic knowledge for designing an effective farm-scale dairy manure treatment system.

  2. Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells

    Directory of Open Access Journals (Sweden)

    F. G. Bystrov

    2016-01-01

    Full Text Available This work is dedicated to the study of the photoluminescence kinetics of aluminum phthalocyanine nanoparticles in colloidal solutions at different pH and in the interaction with immune cells (macrophages. For measurements we used a registration system based on Hamamatsu streak camera (C10627-13 Hamamatsu Photonics with picosecond temporal resolution (15 ps, conjugated with the fiberoptic spectrometer and picosecond laser pumping. The changes in fluorescence decay kinetics as additional lifetime components of fluorescence were found during the experiment. The number of components and duration of lifetimes changed while interacting with cells and depends on pH. At pH 2 the presence of two fluorescence lifetimes was recorded: the first one was 5 ns, which corresponded to the molecular form in solution, and 1.5 ns, which corresponded to bound state of phthalocyanine molecules. Due to the absence of other possible objects for bounding in the solution except of the nanoparticles we can suggest with a high degree of accuracy that the bounding occurs with the very these nanoparticles. Analysis of the fluorescence lifetimes of aluminum phthalocyanine nanoparticles in macrophages indicated the presence of two components: 9 ns and 4.5 ns. A model of surface molecules transitions from parallel to perpendicular position, regarding to the plane of the crystal nanoparticle was proposed.

  3. Multisubstrate kinetics of PAH mixture biodegradation: analysis in the double-logarithmic plot.

    Science.gov (United States)

    Baboshin, Mikhail; Golovleva, Ludmila

    2011-02-01

    The proposed method of kinetic analysis of aqueous-phase biodegradation of polycyclic aromatic hydrocarbons (PAH) mixture presupposes representation of kinetic curves for each pair of mixture components, S(x) and S(y), in double-logarithmic coordinates (ln S(x); ln S(y)). If PAH mixture conversion corresponds to the multisubstrate model with a common active site, then the graphs in double-logarithmic coordinates are straight lines with the angular coefficients equal to the ratio of respective first-order rate constants k(y)(x)= V(y)K(x)/K(y)V(x), where K(x) and K(y) are half-saturation constants, V(x) and V( y ) are the maximum conversion rates for substrates S(x) and S(y); the graph slope does not depend on any concentrations and remains constant during the change of reaction rates as a result of inhibition, induction/inactivation of enzymes or biomass growth. The formulated method has been used to analyze PAH mixture conversion by the culture of Sphingomonas sp. VKM B-2434. It has been shown that this process does not satisfy the multisubstrate model with a single active site. The results suggest that the strain VKM B-2434 contains at least two dioxygenases of different substrate specificity: one enzyme converts phenanthrene and fluoranthene and the other converts acenaphthene and acenaphthylene. The ratios of first-order rate constants have been obtained for these pairs of substrates.

  4. Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production.

    Science.gov (United States)

    Shinto, Hideaki; Tashiro, Yukihiro; Yamashita, Mayu; Kobayashi, Genta; Sekiguchi, Tatsuya; Hanai, Taizo; Kuriya, Yuki; Okamoto, Masahiro; Sonomoto, Kenji

    2007-08-01

    A kinetic simulation model of metabolic pathways that describes the dynamic behaviors of metabolites in acetone-butanol-ethanol (ABE) production by Clostridium saccharoperbutylacetonicum N1-4 was proposed using a novel simulator WinBEST-KIT. This model was validated by comparing with experimental time-course data of metabolites in batch cultures over a wide range of initial glucose concentrations (36.1-295 mM). By introducing substrate inhibition, product inhibition of butanol, activation of butyrate and considering the cessation of metabolic reactions in the case of insufficiency of energy after glucose exhaustion, the revised model showed 0.901 of squared correlation coefficient (r(2)) between experimental time-course of metabolites and calculated ones. Thus, the final revised model is assumed to be one of the best candidates for kinetic simulation describing dynamic behavior of metabolites in ABE production. Sensitivity analysis revealed that 5% increase in reaction of reverse pathway of butyrate production (R(17)) and 5% decrease in reaction of CoA transferase for butyrate (R(15)) highly contribute to high production of butanol. These system analyses should be effective in the elucidation which pathway is metabolic bottleneck for high production of butanol.

  5. Thermogravimetric analysis and kinetics of coal/plastic blends during co-pyrolysis in nitrogen atmosphere

    Energy Technology Data Exchange (ETDEWEB)

    Cai, Junqing; Wang, Yiping; Huang, Qunwu [School of Chemical Engineering and Technology,Tianjin University, Weijin Road No.92, Tianjin 300072 (China); Zhou, Limin [School of Chemical Engineering and Technology,Tianjin University, Weijin Road No.92, Tianjin 300072 (China); Key Laboratory of Nuclear Resources and Environment (East China Institute of Technology), Ministry of Education, Xuefu Road No. 56, Fuzhou, Jiangxi 344000 (China)

    2008-01-15

    Investigations into the co-pyrolytic behaviours of different plastics (high density polyethylene, low density polyethylene and polypropylene), low volatile coal and their blends with the addition of the plastic of 5 wt.% have been conducted using a thermogravimetric analyzer. The results indicated that plastic was decomposed in the temperature range 438-521 C, while the thermal degradation temperature of coal was 174-710 C. The overlapping degradation temperature interval between coal and plastic was favorable for hydrogen transfer from plastic to coal. The difference of weight loss (triangle W) between experimental and theoretical ones, calculated as an algebraic sum of those from each separated component, was 2.0-2.7% at 550-650 C. These experimental results indicated a synergistic effect during plastic and coal co-pyrolysis at the high temperature region. In addition, a kinetic analysis was performed to fit thermogavimetric data, the estimated kinetic parameters (activation energies and pre-exponential factors) for coal, plastic and their blends, were found to be in the range of 35.7-572.8 kJ/mol and 27-1.7 x 10{sup 38} min{sup -} {sup 1}, respectively. (author)

  6. Thermogravimetric Analysis and Kinetics on Reducing Low-Grade Manganese Dioxide Ore by Biomass

    Science.gov (United States)

    Zhang, Honglei; Zhu, Guocai; Yan, Hong; Li, Tiancheng; Feng, Xiujuan

    2013-08-01

    Nonisothermal thermogravimetric analysis (TGA) was applied to evaluate rice straw, sawdust, wheat stalk, maize straw, and bamboo to explore their potential for reduction of manganese dioxide ore. Results from the biomass pyrolysis experiments showed that wood-based biomass materials, such as sawdust and bamboo, could produce more reductive agents, while herb-based biomass materials, such as rice straw, wheat stalk, and maize straw, had lower reaction temperatures. The peak temperatures for biomass reduction tests were 20 K to 50 K (20 °C to 50 °C) higher compared with the pyrolysis tests, and a clear shoulder at around 523 K (250 °C) could be observed. The effects of heating rate, biomass/manganese dioxide ore ratio, and different components of biomass were also investigated. An independent parallel first-order reaction kinetic model was used to calculate the values of activation energy and frequency factor for biomass pyrolysis and reduction of manganese dioxide ore. For better understanding the reduction process, kinetic parameters of independent behavior of manganese dioxide ore were also calculated by simple mathematical treatment. Finally, the isokinetic temperature T i and the rate constant k 0 for reduction of manganese oxide ore by reductive volatiles of biomass were derived according to the Arrhenius equation, which were determined to be 603 K (330 °C) and 108.99 min-1, respectively.

  7. Understanding protein lids: kinetic analysis of active hinge mutants in triosephosphate isomerase.

    Science.gov (United States)

    Sun, J; Sampson, N S

    1999-08-31

    In previous work we tested what three amino acid sequences could serve as a protein hinge in triosephosphate isomerase [Sun, J., and Sampson, N. S. (1998) Protein Sci. 7, 1495-1505]. We generated a genetic library encoding all 8000 possible 3 amino acid combinations at the C-terminal hinge and selected for those combinations of amino acids that formed active mutants. These mutants were classified into six phylogenetic families. Two families resembled wild-type hinges, and four families represented new types of hinges. In this work, the kinetic characteristics and thermal stabilities of mutants representing each of these families were determined in order to understand what properties make an efficient protein hinge, and why all of the families are not observed in nature. From a steady-state kinetic analysis of our mutants, it is clear that the partitioning between protonation of intermediate to form product and intermediate release from the enzyme surface to form methylglyoxal (a decomposition product) is not affected. The two most impaired mutants undergo a change in rate-limiting step from enediol formation to dihydroxyacetone phosphate binding. Thus, it appears that k(cat)/K(m)'s are reduced relative to wild type as a result of slower Michaelis complex formation and dissociation, rather than increased loop opening speed.

  8. Non-isothermal pyrolysis of de-oiled microalgal biomass: Kinetics and evolved gas analysis.

    Science.gov (United States)

    Maurya, Rahulkumar; Ghosh, Tonmoy; Saravaia, Hitesh; Paliwal, Chetan; Ghosh, Arup; Mishra, Sandhya

    2016-12-01

    Non-isothermal (β=5, 10, 20, 35°C/min) pyrolysis of de-oiled microalgal biomass (DMB) of Chlorella variabilis was investigated by TGA-MS (30-900°C, Argon atmosphere) to understand thermal decomposition and evolved gas analysis (EGA). The results showed that three-stage thermal decomposition and three volatilization zone (100-400°C, 400-550°C and 600-750°C) of organic matters during pyrolysis. The highest rate of weight-loss is 8.91%/min at 302°C for 35°C/min heating-rate. Kinetics of pyrolysis were investigated by iso-conversional (KAS, FWO) and model-fitting (Coats-Redfern) method. For Zone-1and3, similar activation energy (Ea) is found in between KAS (α=0.4), FWO (α=0.4) and Avrami-Erofe'ev (n=4) model. Using the best-fitted kinetic model Avrami-Erofe'ev (n=4), Ea values (R(2)=>0.96) are 171.12 (Zone-1), 404.65 (Zone-2) and 691.42kJ/mol (Zone-3). EGA indicate the abundance of most gases observed consequently between 200-300°C and 400-500°C. The pyrolysis of DMB involved multi-step reaction mechanisms for solid-state reactions having different Ea values. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Kinetic analysis and energy efficiency of phenol degradation in a plasma-photocatalysis system.

    Science.gov (United States)

    Wang, Hui-juan; Chen, Xiao-yang

    2011-02-28

    Combination of two kinds of advanced oxidation processes (AOPs) is an effective approach to control wastewater pollution. In this research, a pulsed discharge plasma system with multi-point-to-plate electrode and an immobilized TiO(2) photocatalysis system is coupled to oxidize target pollutant in aqueous solution. Kinetic analysis (pseudo-first order kinetic constant, k) and energy efficiency (energy yield value at 50% phenol conversion, G(50)) of phenol oxidation in different reaction systems (plasma alone and plasma-photocatalysis) are reviewed to account for the synergistic mechanism of plasma and photocatalysis. The experimental results show that higher k and G(50) of phenol oxidation can be obtained in the plasma-photocatalysis system under the conditions of different gas bubbling varieties, initial solution pH and radical scavenger addition. Moreover, the investigation tested hydroxyl radical (OH) is the most important species for phenol removal in the synergistic system of plasma-photocatalysis as well as in the plasma alone system.

  10. Assay of phospholipases A2 and their inhibitors by kinetic analysis in the scooting mode

    Directory of Open Access Journals (Sweden)

    Mahendra Kumar Jain

    1992-01-01

    Full Text Available Several cellular processes are regulated by interfacial catalysis on biomembrane surfaces. Phospholipases A2 (PLA2 are interesting not only as prototypes for interfacial catalysis, but also because they mobilize precursors for the biosynthesis of eicosanoids and platelet activating factor, and these agents ultimately control a wide range of secretory and inflammatory processes. Since PLA2 carry out their catalytic function at membrane surfaces, the kinetics of these enzymes depends on what the enzyme ‘sees’ at the interface, and thus the observed rate is profoundly influenced by the organization and dynamics of the lipidwater interface (‘quality of the interface’. In this review we elaborate the advantages of monitoring interfacial catalysis in the scooting mode, that is, under the conditions where the enzyme remains bound to vesicles for several thousand catalytic turnover cycles. Such a highly processive catalytic turnover in the scooting mode is useful for a rigorous and quantitative characterization of the kinetics of interfacial catalysis. This analysis is now extended to provide insights into designing strategy for PLA2 assays and screens for their inhibitors.

  11. Preliminary failure modes and effects analysis on Korean HCCR TBS to be tested in ITER

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, Mu-Young, E-mail: myahn74@nfri.re.kr [National Fusion Research Institute, Daejeon (Korea, Republic of); Cho, Seungyon [National Fusion Research Institute, Daejeon (Korea, Republic of); Jin, Hyung Gon; Lee, Dong Won [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Park, Yi-Hyun; Lee, Youngmin [National Fusion Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    Highlights: • Postulated initiating events are identified through failure modes and effects analysis on the current HCCR TBS design. • A set of postulated initiating events are selected for consideration of deterministic analysis. • Accident evolutions on the selected postualted initiating events are qualitatively described for deterministic analysis. - Abstract: Korean Helium cooled ceramic reflector (HCCR) Test blanket system (TBS), which comprises Test blanket module (TBM) and ancillary systems in various locations of ITER building, is operated at high temperature and pressure with decay heat. Therefore, safety is utmost concern in design process and it is required to demonstrate that the HCCR TBS is designed to comply with the safety requirements and guidelines of ITER. Due to complexity of the system with many interfaces with ITER, a systematic approach is necessary for safety analysis. This paper presents preliminary failure modes and effects analysis (FMEA) study performed for the HCCR TBS. FMEA is a systematic methodology in which failure modes for components in the system and their consequences are studied from the bottom-up. Over eighty failure modes have been investigated on the HCCR TBS. The failure modes that have similar consequences are grouped as postulated initiating events (PIEs) and total seven reference accident scenarios are derived from FMEA study for deterministic accident analysis. Failure modes not covered here due to evolving design of the HCCR TBS and uncertainty in maintenance procedures will be studied further in near future.

  12. Expression, purification, crystallization and preliminary crystallographic analysis of the proliferation-associated protein Ebp1

    Energy Technology Data Exchange (ETDEWEB)

    Kowalinski, Eva; Bange, Gert; Wild, Klemens; Sinning, Irmgard, E-mail: irmi.sinning@bzh.uni-heidelberg.de [Heidelberg University Biochemistry Center, INF 328, D-69120 Heidelberg (Germany)

    2007-09-01

    Preliminary X-ray analysis of the proliferation-associated protein Ebp1 from Homo sapiens is provided. ErbB-3-binding protein 1 (Ebp1) is a member of the family of proliferation-associated 2G4 proteins (PA2G4s) and plays a role in cellular growth and differentiation. Ligand-induced activation of the transmembrane receptor ErbB3 leads to dissociation of Ebp1 from the receptor in a phosphorylation-dependent manner. The non-associated protein is involved in transcriptional and translational regulation in the cell. Here, the overexpression, purification, crystallization and preliminary crystallographic studies of Ebp1 from Homo sapiens are reported. Initially observed crystals were improved by serial seeding to single crystals suitable for data collection. The optimized crystals belong to the tetragonal space group P4{sub 1}2{sub 1}2 or P4{sub 3}2{sub 1}2 and diffracted to a resolution of 1.6 Å.

  13. Kinematic and kinetic analysis of two gymnastics acrobatic series to performing the backward stretched somersault.

    Science.gov (United States)

    Mkaouer, Bessem; Jemni, Monèm; Amara, Samiha; Chaabène, Helmi; Tabka, Zouhair

    2013-01-01

    Back swing connections during gymnastics acrobatic series considerably influence technical performance and difficulties, particularly in the back somersault. The aim of this study was to compare the take-off's kinetic and kinematic variables between two acrobatic series leading to perform the backward stretched somersault (also called salto): round-off, flic-flac to stretched salto versus round-off, tempo-salto to stretched salto. Five high level male gymnasts (age 23.17 ± 1.61 yrs; body height 1.65 ± 0.05 m; body mass 56.80 ± 7.66 kg) took part in this investigation. A force plate synchronized with a two dimensional movement analysis system was used to collect kinetic and kinematic data. Statistical analysis via the non-parametric Wilcoxon Rank-sum test showed significant differences between the take-offs' variables. The backswing connections were different in the take-off angle, linear momentum, vertical velocity and horizontal and vertical displacements. In conclusion, considering that the higher elevation of the centre of mass in the flight phase would allow best performance and lower the risk of falls, particularly when combined to a great angular momentum, this study demonstrated that the optimal connection series was round-off, flic-flac to stretched salto which enabled the best height in the somersault. Analysis of the results suggests that both connections facilitate the performance of single and double (or triple) backward somersaults with or without rotations around the longitudinal axis. Gymnasts could perform these later while gaining height if they chose the round-off, flic-flac technique or gaining some backward displacement if they choose the round-off, salto tempo.

  14. Wetting of biopolymer coatings: contact angle kinetics and image analysis investigation.

    Science.gov (United States)

    Farris, Stefano; Introzzi, Laura; Biagioni, Paolo; Holz, Torsten; Schiraldi, Alberto; Piergiovanni, Luciano

    2011-06-21

    The surface wetting of five biopolymers, used as coating materials for a plastic film, was monitored over a span of 8 min by means of the optical contact angle technique. Because most of the total variation was observed to occur during the first 60 s, we decided to focus on this curtailed temporal window. Initial contact angle values (θ(0)) ranged from ∼91° for chitosan to ∼30° for pullulan. However, the water drop profile began to change immediately following drop deposition for all biocoatings, confirming that the concept of water contact angle equilibrium is not applicable to most biopolymers. First, a three-parameter decay equation [θ(t) = θ(0) exp(kt(n))] was fit to the experimental contact angle data to describe the kinetics of the contact angle change for each biocoating. Interestingly, the k constant correlated well with the contact angle evolution rate and the n exponent seemed to be somehow linked to the physicochemical phenomena underlying the overall kinetics process. Second, to achieve a reliable description of droplet evolution, the contact angle (CA) analysis was coupled with image analysis (IA) through a combined geometric/trigonometric approach. Absorption and spreading were the key factors governing the overall mechanism of surface wetting during the 60 s analysis, although the individual quantification of both phenomena demonstrated that spreading provided the largest contribution for all biopolymers, with the only exception of gelatin, which showed two quasi-equivalent and counterbalancing effects. The possible correlation between these two phenomena and the topography of the biopolymer surfaces are then discussed on the basis of atomic force microscopy analyses.

  15. A practical approach to the sensitivity analysis for kinetic Monte Carlo simulation of heterogeneous catalysis

    Science.gov (United States)

    Hoffmann, Max J.; Engelmann, Felix; Matera, Sebastian

    2017-01-01

    Lattice kinetic Monte Carlo simulations have become a vital tool for predictive quality atomistic understanding of complex surface chemical reaction kinetics over a wide range of reaction conditions. In order to expand their practical value in terms of giving guidelines for the atomic level design of catalytic systems, it is very desirable to readily evaluate a sensitivity analysis for a given model. The result of such a sensitivity analysis quantitatively expresses the dependency of the turnover frequency, being the main output variable, on the rate constants entering the model. In the past, the application of sensitivity analysis, such as degree of rate control, has been hampered by its exuberant computational effort required to accurately sample numerical derivatives of a property that is obtained from a stochastic simulation method. In this study, we present an efficient and robust three-stage approach that is capable of reliably evaluating the sensitivity measures for stiff microkinetic models as we demonstrate using the CO oxidation on RuO2(110) as a prototypical reaction. In the first step, we utilize the Fisher information matrix for filtering out elementary processes which only yield negligible sensitivity. Then we employ an estimator based on the linear response theory for calculating the sensitivity measure for non-critical conditions which covers the majority of cases. Finally, we adapt a method for sampling coupled finite differences for evaluating the sensitivity measure for lattice based models. This allows for an efficient evaluation even in critical regions near a second order phase transition that are hitherto difficult to control. The combined approach leads to significant computational savings over straightforward numerical derivatives and should aid in accelerating the nano-scale design of heterogeneous catalysts.

  16. Potentiometric sensors doped with biomolecules as a new approach to small molecule/biomolecule binding kinetics analysis.

    Science.gov (United States)

    Daems, D; De Wael, K; Vissenberg, K; Van Camp, G; Nagels, L

    2014-04-15

    The most successful binding kinetics analysis systems at this moment include surface plasmon resonance (SPR), quartz microcrystal balance (QMB) and surface acoustic wave (SAW). Although these are powerful methods, they generally are complex, expensive and require the use of monolayers. Here, we report on potentiometric sensors as an inexpensive and simple alternative to do binding kinetics analysis between small molecules in solution and biomolecules (covalently) attached in a biopolymer sensor coating layer. As an example, dopamine and an anti-dopamine aptamer were used as the small molecule and the biomolecule respectively. Binding between both follows a Langmuir adsorption type model and creates a surface potential. The system operates in Flow Injection Analysis mode (FIA). Besides being an interesting new binding kinetics tool, the approach allows systematic design of potentiometric biosensors (in the present study a dopamine sensor), and gives new insights into the functioning of ion-selective electrodes (ISE's).

  17. PRELIMINARY STUDY ON THE KINETIC PROFILE OF SULFAMETHAZINE IN ADULT LOCAL BREED DOG KAJIAN AWAL TENTANG PROFIL KINETIK SULFAMETAZIN PADA PERANAKAN ANJING LOKAL DEWASA

    Directory of Open Access Journals (Sweden)

    Mochamad Lazuardi

    2007-06-01

    Full Text Available The kinetic parameters of sulmethazine in blood plasma were investigated by UV-Vis spectrophotometer at vaious times following intramuscular administration of 50 mg/kg body weight in local breed dog (Surabaya breed x DEnpasar breed. The objectives of this research

  18. PRELIMINARY STUDY ON THE KINETIC PROFILE OF SULFAMETHAZINE IN ADULT LOCAL BREED DOG KAJIAN AWAL TENTANG PROFIL KINETIK SULFAMETAZIN PADA PERANAKAN ANJING LOKAL DEWASA

    OpenAIRE

    Mochamad Lazuardi

    2007-01-01

    The kinetic parameters of sulmethazine in blood plasma were investigated by UV-Vis spectrophotometer at vaious times following intramuscular administration of 50 mg/kg body weight in local breed dog (Surabaya breed x DEnpasar breed). The objectives of this research

  19. Thermodynamic and chemical kinetic analysis of a 5 kw, compact steam reformer - PEMFC system

    Energy Technology Data Exchange (ETDEWEB)

    Acevedo, Luis Evelio Garcia; Oliveira, Amir Antonio Martins [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil). Dept. de Engenharia Mecanica], e-mail: evelio@labcet.ufsc.br, e-mail: amirol@emc.ufsc.br

    2006-07-01

    Here we present a thermodynamic and chemical kinetic analysis of the methane steam reforming for production of 5 kw of electrical power in a PEM fuel cell. The equilibrium analysis is based on the method of element potentials to find the state of minimum Gibbs free energy for the system and provides the equilibrium concentration of the reforming products. The objective of this analysis is to obtain the range of reforming temperature, pressure and steam-methane molar ratio that results in maximum hydrogen production subjected to low carbon monoxide production and negligible coke formation. The thermal analysis provides the heat transfer rates associated with the individual processes of steam production, gas-phase superheating and reforming necessary to produce 5 kw of electrical power in a PEM fuel cell and allows for the calculation of thermal efficiencies. Then, the chemical reaction pathways for hydrogen production in steam reforming are discussed and the available chemical, adsorption and equilibrium constants are analyzed in terms of thermodynamic consistency. This analysis provides the framework for the reactor sizing and for establishing the adequate operation conditions. (author)

  20. A steady-state kinetic analysis of the prolyl-4-hydroxylase mechanism.

    Science.gov (United States)

    Soskel, N T; Kuby, S A

    1981-01-01

    Published kinetic data by Kivirikko, et al. on the prolyl-4-hydroxylase reaction have been re-evaluated using the overall steady-state velocity equation in the forward and reverse directions for an ordered ter ter kinetic mechanism. Qualitatively, the published data for prolyl-4-hydroxylase appear to fit the predicted patterns for this kinetic mechanism. More kinetic data are needed to confirm these results and to quantitate the kinetic parameters but, tentatively, the order of substrate addition would appear to be alpha-ketoglutarate, oxygen, and peptide; and the order of product release would be hydroxylated peptide (or collagen), carbon dioxide, and succinate.

  1. Kinetics analysis and quantitative calculations for the successive radioactive decay process

    Science.gov (United States)

    Zhou, Zhiping; Yan, Deyue; Zhao, Yuliang; Chai, Zhifang

    2015-01-01

    The general radioactive decay kinetics equations with branching were developed and the analytical solutions were derived by Laplace transform method. The time dependence of all the nuclide concentrations can be easily obtained by applying the equations to any known radioactive decay series. Taking the example of thorium radioactive decay series, the concentration evolution over time of various nuclide members in the family has been given by the quantitative numerical calculations with a computer. The method can be applied to the quantitative prediction and analysis for the daughter nuclides in the successive decay with branching of the complicated radioactive processes, such as the natural radioactive decay series, nuclear reactor, nuclear waste disposal, nuclear spallation, synthesis and identification of superheavy nuclides, radioactive ion beam physics and chemistry, etc.

  2. Kinetics analysis and quantitative calculations for the successive radioactive decay process

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, Zhiping, E-mail: zhouzp@ujs.edu.cn [School of Materials Science and Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013 (China); Yan, Deyue [School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240 (China); Zhao, Yuliang; Chai, Zhifang [Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049 (China)

    2015-01-15

    The general radioactive decay kinetics equations with branching were developed and the analytical solutions were derived by Laplace transform method. The time dependence of all the nuclide concentrations can be easily obtained by applying the equations to any known radioactive decay series. Taking the example of thorium radioactive decay series, the concentration evolution over time of various nuclide members in the family has been given by the quantitative numerical calculations with a computer. The method can be applied to the quantitative prediction and analysis for the daughter nuclides in the successive decay with branching of the complicated radioactive processes, such as the natural radioactive decay series, nuclear reactor, nuclear waste disposal, nuclear spallation, synthesis and identification of superheavy nuclides, radioactive ion beam physics and chemistry, etc.

  3. Cell kinetics in a model of artificial skin. An immunohistochemical and flow cytometric analysis

    Directory of Open Access Journals (Sweden)

    A Casasco

    2009-12-01

    Full Text Available Bioengineered organs raised in vitro are candidate substitutes for natural organs in biological, pharmacological and clinical applications. We have studied cell kinetics in a human skin equivalent (HSE using a combined immunohistochemical and flow cytometric approach. Morphological analysis has shown that, relative to unstimulated natural skin, cell proliferation mainly occurs in the basal layer of the epidermal equivalent. Immunohistochemical and flow cytometric measurements of the growth fraction suggested a cell turnover comparable to that of natural skin. Immunohistochemical labelling indices matched well with flow cytometric data. These observations are consistent with morphological and histochemical data demonstrating normal cell differentiation and tissue architecture in HSE and suggest that such HSE may be a usefull substitute for human skin.

  4. Upper limb joint kinetic analysis during tennis serve: Assessment of competitive level on efficiency and injury risks.

    Science.gov (United States)

    Martin, C; Bideau, B; Ropars, M; Delamarche, P; Kulpa, R

    2014-08-01

    The aim of this work was to compare the joint kinetics and stroke production efficiency for the shoulder, elbow, and wrist during the serve between professionals and advanced tennis players and to discuss their potential relationship with given overuse injuries. Eleven professional and seven advanced tennis players were studied with an optoelectronic motion analysis system while performing serves. Normalized peak kinetic values of the shoulder, elbow, and wrist joints were calculated using inverse dynamics. To measure serve efficiency, all normalized peak kinetic values were divided by ball velocity. t-tests were used to determine significant differences between the resultant joint kinetics and efficiency values in both groups (advanced vs professional). Shoulder inferior force, shoulder anterior force, shoulder horizontal abduction torque, and elbow medial force were significantly higher in advanced players. Professional players were more efficient than advanced players, as they maximize ball velocity with lower joint kinetics. Since advanced players are subjected to higher joint kinetics, the results suggest that they appeared more susceptible to high risk of shoulder and elbow injuries than professionals, especially during the cocking and deceleration phases of the serve.

  5. Biosynthesis and stereoselective analysis of (-)- and (+)-zaltoprofen glucuronide in rat hepatic microsomes and its application to the kinetic analysis.

    Science.gov (United States)

    Wang, Haina; Ji, Jianbo; Zeng, Su

    2011-08-15

    Zaltoprofen, available commercially as a racemic mixture, is a propionic acid derivative of non-steroidal anti-inflammatory drugs (NSAIDs). Firstly, (+)- and (-)-zaltoprofen glucuronide was biosynthesized and purified. Then a simple and rapid RP-HPLC analysis method for direct determination of (+)- and (-)-zaltoprofen glucuronide in rat hepatic microsomes was developed and validated. The calibration curves of (+)- and (-)-zaltoprofen glucuronide both showed good linearity in the concentration range from 0.15 to 31.13 μM. The lower limit of quantification was 0.15 μM. Finally, this method was used to investigate the enantioselectivity of zaltoprofen glucuronidation in rat hepatic microsomes. The kinetics of zaltoprofen glucuronidation in rat hepatic microsomes for 40 min incubation fit the Michaelis-Menten model. Kinetic analysis indicated that (-)-zaltoprofen had a higher glucuronidation rate in rat liver microsome than that of (+)-zaltoprofen. The catalyzing efficiency (V(max)/K(m)) ratio of (+)-zaltoprofen to (-)-enantiomer is 0.8 times in rat liver microsomes.

  6. Kinetic analysis of lauric acid hydroxylation by human cytochrome P450 4A11.

    Science.gov (United States)

    Kim, Donghak; Cha, Gun-Su; Nagy, Leslie D; Yun, Chul-Ho; Guengerich, F Peter

    2014-10-07

    Cytochrome P450 (P450) 4A11 is the only functionally active subfamily 4A P450 in humans. P450 4A11 catalyzes mainly ω-hydroxylation of fatty acids in liver and kidney; this process is not a major degradative pathway, but at least one product, 20-hydroxyeicosatetraenoic acid, has important signaling properties. We studied catalysis by P450 4A11 and the issue of rate-limiting steps using lauric acid ω-hydroxylation, a prototypic substrate for this enzyme. Some individual reaction steps were studied using pre-steady-state kinetic approaches. Substrate and product binding and release were much faster than overall rates of catalysis. Reduction of ferric P450 4A11 (to ferrous) was rapid and not rate-limiting. Deuterium kinetic isotope effect (KIE) experiments yielded low but reproducible values (1.2-2) for 12-hydroxylation with 12-(2)H-substituted lauric acid. However, considerable "metabolic switching" to 11-hydroxylation was observed with [12-(2)H3]lauric acid. Analysis of switching results [Jones, J. P., et al. (1986) J. Am. Chem. Soc. 108, 7074-7078] and the use of tritium KIE analysis with [12-(3)H]lauric acid [Northrop, D. B. (1987) Methods Enzymol. 87, 607-625] both indicated a high intrinsic KIE (>10). Cytochrome b5 (b5) stimulated steady-state lauric acid ω-hydroxylation ∼2-fold; the apoprotein was ineffective, indicating that electron transfer is involved in the b5 enhancement. The rate of b5 reoxidation was increased in the presence of ferrous P450 mixed with O2. Collectively, the results indicate that both the transfer of an electron to the ferrous·O2 complex and C-H bond-breaking limit the rate of P450 4A11 ω-oxidation.

  7. Analysis of IgG kinetic stability by differential scanning calorimetry, probe fluorescence and light scattering.

    Science.gov (United States)

    Nemergut, Michal; Žoldák, Gabriel; Schaefer, Jonas V; Kast, Florian; Miškovský, Pavol; Plückthun, Andreas; Sedlák, Erik

    2017-08-19

    Monoclonal antibodies of the immunoglobulin G (IgG) type have become mainstream therapeutics for the treatment of many life-threatening diseases. For their successful application in the clinic and a favorable cost-benefit ratio, the design and formulation of these therapeutic molecules must guarantee long-term stability for an extended period of time. Accelerated stability studies, e.g., by employing thermal denaturation, have the great potential for enabling high-throughput screening campaigns to find optimal molecular variants and formulations in a short time. Surprisingly, no validated quantitative analysis of these accelerated studies has been performed yet, which clearly limits their application for predicting IgG stability. Therefore, we have established a quantitative approach for the assessment of the kinetic stability over a broad range of temperatures. To this end, differential scanning calorimetry (DSC) experiments were performed with a model IgG, testing chaotropic formulations and an extended temperature range, and they were subsequently analyzed by our recently developed three-step sequential model of IgG denaturation, consisting of one reversible and two irreversible steps. A critical comparison of the predictions from this model with data obtained by an orthogonal fluorescence probe method, based on 8-anilinonaphthalene-1-sulfonate binding to partially unfolded states, resulted in very good agreement. In summary, our study highlights the validity of this easy-to-perform analysis for reliably assessing the kinetic stability of IgGs, which can support accelerated formulation development of monoclonal antibodies by ranking different formulations as well as by improving colloidal stability models. © 2017 The Protein Society.

  8. Preliminary Analysis on the Relative Solution Space Sizes for MTSP with Genetic Algorithm

    Science.gov (United States)

    Hao, Junling

    It is well known that the chromosome design is pivotal to solve the multiple traveling salesman problems with genetic algorithm. A well-designed chromosome coding can eliminate or reduce the redundant solutions. One chromosome and two chromosome design methods and a recently proposed two-part chromosome design are firstly introduced in this paper. Then the preliminary quantitative comparison analysis of the solution spaces of three different chromosome design methods is presented when the number of cities is linear with the travelers. The concept of relative solution space is proposed in order to compare the relative size of the solution spaces. The solution space of two-part chromosome design is much smaller than those of the traditional chromosome design. The result given in this paper provides a good guideline for the possible algorithmic design and engineering applications.

  9. Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.

    Science.gov (United States)

    Maruyama, Yukie; Itoh, Takafumi; Nishitani, Yu; Mikami, Bunzo; Hashimoto, Wataru; Murata, Kousaku

    2012-03-01

    Sphingomonas sp. A1 directly incorporates alginate polysaccharides through a 'superchannel' comprising a pit on the cell surface, alginate-binding proteins in the periplasm and an ABC transporter (alginate importer) in the inner membrane. Alginate importer, consisting of four subunits, AlgM1, AlgM2 and two molecules of AlgS, was crystallized in the presence of the binding protein AlgQ2. Preliminary X-ray analysis showed that the crystal diffracted to 3.3 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 72.5, b = 136.8, c = 273.3 Å, suggesting the presence of one complex in the asymmetric unit.

  10. ANSI/ASHRAE/IES Standard 90.1-2013 Preliminary Determination: Qualitative Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Halverson, Mark A.; Hart, Reid; Athalye, Rahul A.; Rosenberg, Michael I.; Richman, Eric E.; Winiarski, David W.

    2014-03-01

    Section 304(b) of the Energy Conservation and Production Act (ECPA), as amended, requires the Secretary of Energy to make a determination each time a revised version of ASHRAE Standard 90.1 is published with respect to whether the revised standard would improve energy efficiency in commercial buildings. When the U.S. Department of Energy (DOE) issues an affirmative determination on Standard 90.1, states are statutorily required to certify within two years that they have reviewed and updated the commercial provisions of their building energy code, with respect to energy efficiency, to meet or exceed the revised standard. This report provides a preliminary qualitative analysis of all addenda to ANSI/ASHRAE/IES Standard 90.1-2010 (referred to as Standard 90.1-2010 or 2010 edition) that were included in ANSI/ASHRAE/IES Standard 90.1-2013 (referred to as Standard 90.1-2013 or 2013 edition).

  11. Crystallization and preliminary X-ray analysis of Rv1674c from Mycobacterium tuberculosis

    Science.gov (United States)

    Li, Jincheng; Wang, Xudong; Gong, Weimin; Niu, Chunyan; Zhang, Min

    2015-01-01

    Adaptations to hypoxia play an important role in Mycobacterium tuberculosis pathogenesis. Rv0324, which contains an HTH DNA-binding domain and a rhodanese domain, is one of the key transcription regulators in response to hypoxia. M. tuberculosis Rv1674c is a homologue of Rv0324. To understand the interdomain interaction and regulation of the HTH domain and the rhodanese domain, recombinant Rv1674c protein was purified and crystallized by the vapour-diffusion method. The crystals diffracted to 2.25 Å resolution. Preliminary diffraction analysis suggests that the crystals belonged to space group P3121 or P3221, with unit-cell parameters a = b = 67.8, c = 174.5 Å, α = β = 90, γ = 120°. The Matthews coefficient was calculated to be 2.44 Å3 Da−1, assuming that the crystallographic asymmetric unit contains two protein molecules. PMID:25760714

  12. Preliminary phytochemical analysis, antibacterial, antifungal and anticandidal activities of successive extracts of Crossandra infundibuliformis

    Institute of Scientific and Technical Information of China (English)

    MadhumithaG; SaralAM

    2011-01-01

    Objective:To investigate the phytochemical, antibacterial, antifungal and anticandidal activity of successive extracts of Crossandra infundibuliformis (Acanthaceae) leaves. Methods:Preliminary screening on the presence of alkaloids, saponins, phytosterols, phenolic compounds, flavanoids, tannins, carbohydrates, terpenoids, oils and fats were carried out by phytochemical analysis. The antibacterial, antifungal and anticandidal activities were done by agar well diffusion technique. Results:The successive extracts have an array of chemical constituents and the MIC values of antibacterial activity ranges from 0.007 8 to 0.015 0μg/mL. In case of antifungal and anticandidal activities the MIC values were between 0.125 and 0.250μg/mL. Conclusions:These findings demonstrate that the leaf extracts of C. infundibuliformis presents excellent antimicrobial activities and thus have great potential as a source for natural health care products.

  13. Preliminary C3 Loading Analysis for Future High-Altitude Unmanned Aircraft in the NAS

    Science.gov (United States)

    Ho, Yan-Shek; Gheorghisor, Izabela; Box, Frank

    2006-01-01

    This document provides a preliminary assessment and summary of the command, control, and communications (C(sup 3)) loading requirements of a generic future high-altitude, long-endurance unmanned aircraft (UA) operating at in the National Airspace System. Two principal types of C(sup 3) traffic are considered in our analysis: communications links providing air traffic services (ATS) to the UA and its human pilot, and the command and control data links enabling the pilot to operate the UA remotely. we have quantified the loading requirements of both types of traffic for two different assumed levels of UA autonomy. Our results indicate that the potential use of UA-borne relays for the ATS links, and the degree of autonomy exercised by the UA during the departure and arrival phases of its flight, will be among the key drivers of C(sup 3) loading and bandwidth requirements.

  14. Visual Assessment on Coastal Cruise Tourism: A Preliminary Planning Using Importance Performance Analysis

    Science.gov (United States)

    Trisutomo, S.

    2017-07-01

    Importance-Performance Analysis (IPA) has been widely applied in many cases. In this research, IPA was applied to measure perceive on coastal tourism objects and its possibility to be developed as coastal cruise tourism in Makassar. Three objects, i.e. Akkarena recreational site, Losari public space at waterfront, and Paotere traditional Phinisi ships port, were selected and assessed visually from water area by a group of purposive resource persons. The importance and performance of 10 attributes of each site were scored using Likert scale from 1 to 5. Data were processed by SPSS-21 than resulted Cartesian graph which the scores were divided in four quadrants: Quadrant I concentric here, Quadrant II keep up the good work, Quadrant III low priority, and Quadrant IV possible overkill. The attributes in each quadrant could be considered as the platform for preliminary planning of coastal cruise tour in Makassar

  15. Preliminary control system design and analysis for the Space Station Furnace Facility thermal control system

    Science.gov (United States)

    Jackson, M. E.

    1995-01-01

    This report presents the Space Station Furnace Facility (SSFF) thermal control system (TCS) preliminary control system design and analysis. The SSFF provides the necessary core systems to operate various materials processing furnaces. The TCS is defined as one of the core systems, and its function is to collect excess heat from furnaces and to provide precise cold temperature control of components and of certain furnace zones. Physical interconnection of parallel thermal control subsystems through a common pump implies the description of the TCS by coupled nonlinear differential equations in pressure and flow. This report formulates the system equations and develops the controllers that cause the interconnected subsystems to satisfy flow rate tracking requirements. Extensive digital simulation results are presented to show the flow rate tracking performance.

  16. Acanthamoeba polyphaga mimivirus NDK: preliminary crystallographic analysis of the first viral nucleoside diphosphate kinase.

    Science.gov (United States)

    Jeudy, Sandra; Coutard, Bruno; Lebrun, Régine; Abergel, Chantal

    2005-06-01

    The complete sequence of the largest known double-stranded DNA virus, Acanthamoeba polyphaga mimivirus, has recently been determined [Raoult et al. (2004), Science, 306, 1344-1350] and revealed numerous genes not expected to be found in a virus. A comprehensive structural and functional study of these gene products was initiated [Abergel et al. (2005), Acta Cryst. F61, 212-215] both to better understand their role in the virus physiology and to obtain some clues to the origin of DNA viruses. Here, the preliminary crystallographic analysis of the viral nucleoside diphosphate kinase protein is reported. The crystal belongs to the cubic space group P2(1)3, with unit-cell parameter 99.425 A. The self-rotation function confirms that there are two monomers per asymmetric unit related by a twofold non-crystallographic axis and that the unit cell thus contains four biological entities.

  17. Preliminary analysis of habitat utilization by woodland caribou in northwestern Ontario using satellite telemetry

    Directory of Open Access Journals (Sweden)

    T.L. Hillis

    1998-03-01

    Full Text Available Locational data collected over a one year period from 10 female woodland caribou, Rangifer tarandus caribou, collared with Argos satellite collars in northwestern Ontario, Canada were superimposed on supervised Landsat images using Geographical Information System (GIS technology. Landscape parameters, land cover classifications, and drainage were utilized to create the basemap. Using ARCVIEW software, all digital fixes from collared caribou with information of date, time, and activity status were overlain on the basemap to facilitate a preliminary analysis of habitat use in this species. Results supported the conclusions (1 that woodland caribou in northwestern Ontario select habitats containing high to moderate conifer cover and avoided disturbed areas and shrub-rich habitats, (2 that seasonal changes in habitat utilization occurs in females of this species, and (3 that satellite telemetry technology can be employed in the boreal forest ecosystem to assess habitat utilization by large ungulate species.

  18. Crystallization and preliminary X-ray analysis of Leishmania major glyoxalase I

    Energy Technology Data Exchange (ETDEWEB)

    Ariza, Antonio; Vickers, Tim J.; Greig, Neil; Fairlamb, Alan H.; Bond, Charles S., E-mail: c.s.bond@dundee.ac.uk [Division of Biological Chemistry and Molecular Microbiology, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH,Scotland (United Kingdom)

    2005-08-01

    The detoxification enzyme glyoxalase I from L. major has been crystallized. Preliminary molecular-replacement calculations indicate the presence of three glyoxalase I dimers in the asymmetric unit. Glyoxalase I (GLO1) is a putative drug target for trypanosomatids, which are pathogenic protozoa that include the causative agents of leishmaniasis. Significant sequence and functional differences between Leishmania major and human GLO1 suggest that it may make a suitable template for rational inhibitor design. L. major GLO1 was crystallized in two forms: the first is extremely disordered and does not diffract, while the second, an orthorhombic form, produces diffraction to 2.0 Å. Molecular-replacement calculations indicate that there are three GLO1 dimers in the asymmetric unit, which take up a helical arrangement with their molecular dyads arranged approximately perpendicular to the c axis. Further analysis of these data are under way.

  19. Cognitive Work Analysis: Preliminary Data for a Model of Problem Solving Strategies

    Science.gov (United States)

    Rothmayer, Mark; Blue, Jennifer

    2007-10-01

    Investigations into problem solving strategies are part of the field of physics education research where investigators seek to improve physics instruction by conducting basic research of problem solving abilities among students, differences in knowledge representations between experts and novices, and how to transfer knowledge structures more effectively onto novices. We developed a new conceptual research tool in our laboratory, where we could potentially map the step by step flow of problem solving strategies among experts and novices. This model is derived from the theory of Cognitive Work Analysis, which is grounded in ecological psychology, and as far as we know it has never been applied to a knowledge domain like physics. We collected survey data from 140 undergraduates enrolled in an algebra based introductory physics course at Miami University as part of a larger study aimed to test the validity of the model. Preliminary data will be presented and discussed.

  20. Preliminary risk analysis applied to the transmission of Creutzfeldt-Jakob disease.

    Science.gov (United States)

    Bertrand, E; Schlatter, J

    2011-01-01

    Transmissible spongiform encephalopathy (TSE) is a degenerative disease of the central nervous system. As yet, there is no human screening test and no effective treatment. This disease is invariably fatal. General preventive measures are therefore essential. The objective of this study is to analyze and address on a prioritized basis the risks relating to the transmission of Creutzfeldt-Jakob disease during surgical operations by means of a preliminary risk analysis (PRA). The PRA produces 63 scenarios with maximum risk relating to operational and legal dangers. The study recommends a number of courses of action, such as training and internal controls, in order to reduce the risks identified. A procedure has been drawn up and assessed for each action. This PRA makes it possible to target and significantly reduce the potential dangers for transmission of Creutzfeldt-Jakob disease through the use of medical instruments.

  1. Purification, crystallization and preliminary X-ray analysis of uridine phosphorylase from Salmonella typhimurium.

    Science.gov (United States)

    Dontsova, Mariya V; Savochkina, Yulia A; Gabdoulkhakov, Azat G; Baidakov, Sergey N; Lyashenko, Andrey V; Zolotukhina, Maria; Errais Lopes, Liubov; Garber, Mariya B; Morgunova, Ekaterina Yu; Nikonov, Stanislav V; Mironov, Alexandr S; Ealick, Steven E; Mikhailov, Al 'Bert M

    2004-04-01

    The structural udp gene encoding uridine phosphorylase (UPh) was cloned from the Salmonella typhimurium chromosome and overexpressed in Escherichia coli cells. S. typhimurium UPh (StUPh) was purified to apparent homogeneity and crystallized. The primary structure of StUPh has high homology to the UPh from E. coli, but the enzymes differ substantially in substrate specificity and sensitivity to the polarity of the medium. Single crystals of StUPh were grown using hanging-drop vapor diffusion with PEG 8000 as the precipitant. X-ray diffraction data were collected to 2.9 A resolution. Preliminary analysis of the diffraction data indicated that the crystal belonged to space group P6(1(5)), with unit-cell parameters a = 92.3, c = 267.5 A. The solvent content is 37.7% assuming the presence of one StUPh hexamer per asymmetric unit.

  2. Crystallization and preliminary X-ray diffraction analysis of West Nile virus

    Energy Technology Data Exchange (ETDEWEB)

    Kaufmann, Barbel; Plevka, Pavel; Kuhn, Richard J.; Rossmann, Michael G. (Purdue)

    2010-05-25

    West Nile virus, a human pathogen, is closely related to other medically important flaviviruses of global impact such as dengue virus. The infectious virus was purified from cell culture using polyethylene glycol (PEG) precipitation and density-gradient centrifugation. Thin amorphously shaped crystals of the lipid-enveloped virus were grown in quartz capillaries equilibrated by vapor diffusion. Crystal diffraction extended at best to a resolution of about 25 {angstrom} using synchrotron radiation. A preliminary analysis of the diffraction images indicated that the crystals had unit-cell parameters a {approx_equal} b {approx_equal} 480 {angstrom}, {gamma} = 120{sup o}, suggesting a tight hexagonal packing of one virus particle per unit cell.

  3. Preliminary performance analysis of the advanced pulse compression noise radar waveform

    Science.gov (United States)

    Govoni, Mark A.; Moyer, Lee R.

    2012-06-01

    Noise radar systems encounter target fluctuation behavior similar to that of conventional systems. For noise radar systems, however, the fluctuations are not only dictated by target composition and geometry, but also by the non-uniform power envelope of their random transmit signals. This third dependency is of interest and serves as the basis for the preliminary analysis conducted in this manuscript. General conclusions are drawn on the implications of having a random power envelope and the impacts it could have on both the transmit and receive processes. Using an advanced pulse compression noise (APCN) radar waveform as the constituent signal, a computer simulation aids in quantifying potential losses and the impacts they might have on the detection performance of a real radar system.

  4. Preliminary Analysis of Aircraft Loss of Control Accidents: Worst Case Precursor Combinations and Temporal Sequencing

    Science.gov (United States)

    Belcastro, Christine M.; Groff, Loren; Newman, Richard L.; Foster, John V.; Crider, Dennis H.; Klyde, David H.; Huston, A. McCall

    2014-01-01

    Aircraft loss of control (LOC) is a leading cause of fatal accidents across all transport airplane and operational classes, and can result from a wide spectrum of hazards, often occurring in combination. Technologies developed for LOC prevention and recovery must therefore be effective under a wide variety of conditions and uncertainties, including multiple hazards, and their validation must provide a means of assessing system effectiveness and coverage of these hazards. This requires the definition of a comprehensive set of LOC test scenarios based on accident and incident data as well as future risks. This paper defines a comprehensive set of accidents and incidents over a recent 15 year period, and presents preliminary analysis results to identify worst-case combinations of causal and contributing factors (i.e., accident precursors) and how they sequence in time. Such analyses can provide insight in developing effective solutions for LOC, and form the basis for developing test scenarios that can be used in evaluating them. Preliminary findings based on the results of this paper indicate that system failures or malfunctions, crew actions or inactions, vehicle impairment conditions, and vehicle upsets contributed the most to accidents and fatalities, followed by inclement weather or atmospheric disturbances and poor visibility. Follow-on research will include finalizing the analysis through a team consensus process, defining future risks, and developing a comprehensive set of test scenarios with correlation to the accidents, incidents, and future risks. Since enhanced engineering simulations are required for batch and piloted evaluations under realistic LOC precursor conditions, these test scenarios can also serve as a high-level requirement for defining the engineering simulation enhancements needed for generating them.

  5. Exact and user-friendly kinetic analysis of the two-step rapid equilibrium Michaelis-Menten mechanism.

    Science.gov (United States)

    Garneau-Tsodikova, Sylvie; Shkel, Irina A; Tsodikov, Oleg V

    2009-04-15

    Most enzyme kinetic experiments are carried out under pseudo-first-order conditions, that is, when one of the reactant species (the enzyme or the substrate) is in a large excess of the other species. More accurate kinetic information about the system can be gained without the restrictions of the pseudo-first-order conditions. We present a practical and general method of analysis of the common two-step rapid equilibrium Michaelis-Menten mechanism. The formalism is exact in that it does not involve any other approximations such as the steady-state, limitations on the reactant concentrations or on reaction times. We apply this method to the global analysis of kinetic progress curves for bovine alkaline phosphatase assays carried out under both pseudo-first-order and pseudo-second-order conditions.

  6. Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes

    OpenAIRE

    Jan David Kijlstra; Dongjian Hu; Nikhil Mittal; Eduardo Kausel; Peter van der Meer; Arman Garakani; Ibrahim J. Domian

    2015-01-01

    Summary The quantitative analysis of cardiomyocyte function is essential for stem cell-based approaches for the in vitro study of human cardiac physiology and pathophysiology. We present a method to comprehensively assess the function of single human pluripotent stem cell-derived cardiomyocyte (hPSC-CMs) through simultaneous quantitative analysis of contraction kinetics, force generation, and electrical activity. We demonstrate that statistical analysis of movies of contracting hPSC-CMs can b...

  7. LES and Proper Orthogonal Decomposition analysis of vertical entrainment of kinetic energy in large wind farms (Invited)

    Science.gov (United States)

    Meneveau, C. V.; VerHulst, C.

    2013-12-01

    Vertical entrainment of kinetic energy has been shown to be an important limiting factor in the performance of very large wind turbine arrays. Given high Reynolds numbers and domain sizes on the order of kilometers, we rely on wall-modeled Large Eddy Simulation (LES) to predict flow within large wind farm. We use Proper Orthogonal Decomposition (POD) to identify energetically important large-scale structures in the flow. The primary large-scale structures are found to be streamwise counter-rotating vortices located above the height of the wind turbines. The contribution of each flow structure to the kinetic energy entrainment is quantified. Surprisingly, fewer flow structures (POD modes) contribute to the vertical kinetic energy flux than to the kinetic energy in the flow, for which the POD analysis is optimal. While the general characteristics of the flow structures are robust, the net kinetic energy entrainment to the turbines depends on the orientation of the wind turbines in the array. The various modes' contributions to variability and intermittency is also quantified. The POD analysis is performed for aligned and staggered wind turbine arrays as well as for atmospheric flow without wind turbines. This research is supported by a NSF Graduate Fellowship and by the WINDINSPIRE project, funded through NSF-OISE 1243482.

  8. A model for the occurrence and analysis of ionic thermocurrent spectrum involving different orders of kinetics

    Indian Academy of Sciences (India)

    Jai Prakash

    2013-01-01

    Ionic thermocurrent (ITC) spectrum is much similar to a thermoluminescence (TL) glow curve involving monomolecular kinetics. It has already been reported that different orders of kinetics are involved in TL processes, which depend specifically on the extent of recombination and simultaneous retrapping. It is found that the involvement of different orders of kinetics in ITC spectrum depends on the experimental conditions of polarization and rate of rapid cooling. Consequently, order of kinetics involved in the ITC spectrum does not represent any specific feature of the system under investigation. An equation is developed which explains the occurrence of ITC spectrum involving any order of kinetics. Dielectric relaxation parameters, order of kinetics and approximate number of dipoles per unit volume are evaluated conveniently and easily following the proposed model.

  9. Modal and Dynamic Analysis of a Vehicle with Kinetic Dynamic Suspension System

    Directory of Open Access Journals (Sweden)

    Bangji Zhang

    2016-01-01

    Full Text Available A novel kinetic dynamic suspension (KDS system is presented for the cooperative control of the roll and warp motion modes of off-road vehicles. The proposed KDS system consists of two hydraulic cylinders acting on the antiroll bars. Hence, the antiroll bars are not completely replaced by the hydraulic system, but both systems are installed. In this paper, the vibration analysis in terms of natural frequencies of different motion modes in frequency domain for an off-road vehicle equipped with different configurable suspension systems is studied by using the modal analysis method. The dynamic responses of the vehicle with different configurable suspension systems are investigated under different road excitations and maneuvers. The results of the modal and dynamic analysis prove that the KDS system can reduce the roll and articulation motions of the off-road vehicle without adding extra bounce stiffness and deteriorating the ride comfort. Furthermore, the roll stiffness is increased and the warp stiffness is decreased by the KDS system, which could significantly enhance handing performance and off-road capability.

  10. Neutron activation analysis via nuclear decay kinetics using gamma-ray spectroscopy at SFU

    Science.gov (United States)

    Domingo, Thomas; Chester, Aaron; Starosta, Krzysztof; Williams, Jonathan

    2016-09-01

    Gamma-ray spectroscopy is a powerful tool used in a variety of fields including nuclear and analytical chemistry, environmental science, and health risk management. At SFU, the Germanium detector for Elemental Analysis and Radiation Studies (GEARS), a low-background shielded high-purity germanium gamma-ray detector, has been used recently in all of the above fields. The current project aims to expand upon the number of applications for which GEARS can be used while enhancing its current functionality. A recent addition to the SFU Nuclear Science laboratory is the Thermo Scientific P 385 neutron generator. This device provides a nominal yield of 3 ×108 neutrons/s providing the capacity for neutron activation analysis, opening a major avenue of research at SFU which was previously unavailable. The isotopes created via neutron activation have a wide range of half-lives. To measure and study isotopes with half-lives above a second, a new analogue data acquisition system has been installed on GEARS allowing accurate measurements of decay kinetics. This new functionality enables identification and quantification of the products of neutron activation. Results from the neutron activation analysis of pure metals will be presented.

  11. Inverse problem analysis for identification of reaction kinetics constants in microreactors for biodiesel synthesis

    Science.gov (United States)

    Pontes, P. C.; Naveira-Cotta, C. P.

    2016-09-01

    The theoretical analysis for the design of microreactors in biodiesel production is a complicated task due to the complex liquid-liquid flow and mass transfer processes, and the transesterification reaction that takes place within these microsystems. Thus, computational simulation is an important tool that aids in understanding the physical-chemical phenomenon and, consequently, in determining the suitable conditions that maximize the conversion of triglycerides during the biodiesel synthesis. A diffusive-convective-reactive coupled nonlinear mathematical model, that governs the mass transfer process during the transesterification reaction in parallel plates microreactors, under isothermal conditions, is here described. A hybrid numerical-analytical solution via the Generalized Integral Transform Technique (GITT) for this partial differential system is developed and the eigenfunction expansions convergence rates are extensively analyzed and illustrated. The heuristic method of Particle Swarm Optimization (PSO) is applied in the inverse analysis of the proposed direct problem, to estimate the reaction kinetics constants, which is a critical step in the design of such microsystems. The results present a good agreement with the limited experimental data in the literature, but indicate that the GITT methodology combined with the PSO approach provide a reliable computational algorithm for direct-inverse analysis in such reactive mass transfer problems.

  12. Kinetics of oxidation of H2 and reduction of H2O in Ni-YSZ based solid oxide cells

    DEFF Research Database (Denmark)

    Ebbesen, Sune Dalgaard; Mogensen, Mogens Bjerg

    2012-01-01

    to oxidation of H2. The kinetic differences cannot be explained by the reaction mechanisms which are similar in the two cases but are rather an effect of the thermodynamics. The preliminary analysis performed in this study show that the slow kinetic for reduction is partly related to the endothermic nature...... of the reaction, cooling the active electrode, thereby leading to slower kinetics at low current densities. Likewise, the increased kinetic for oxidation was found to be related to the exothermic nature of the reaction, heating the active electrode, and thereby leading to faster kinetics. At higher current...

  13. Preliminary Safety Analysis Report for the Transuranic Storage Area Retrieval Enclosure at the Idaho National Engineering Laboratory. Revision 8

    Energy Technology Data Exchange (ETDEWEB)

    1993-03-01

    This Transuranic Storage Area Retrieval Enclosure Preliminary Safety Analysis Report was completed as required by DOE Order 5480.23. The purpose of this document is to construct a safety basis that supports the design and permits construction of the facility. The facility has been designed to the requirements of a Radioactive Solid Waste Facility presented in DOE Order 6430.1A.

  14. Yucca Mountain transportation routes: Preliminary characterization and risk analysis; Volume 2, Figures [and] Volume 3, Technical Appendices

    Energy Technology Data Exchange (ETDEWEB)

    Souleyrette, R.R. II; Sathisan, S.K.; di Bartolo, R. [Nevada Univ., Las Vegas, NV (United States). Transportation Research Center

    1991-05-31

    This report presents appendices related to the preliminary assessment and risk analysis for high-level radioactive waste transportation routes to the proposed Yucca Mountain Project repository. Information includes data on population density, traffic volume, ecologically sensitive areas, and accident history.

  15. Yucca Mountain transportation routes: Preliminary characterization and risk analysis; Volume 2, Figures [and] Volume 3, Technical Appendices

    Energy Technology Data Exchange (ETDEWEB)

    Souleyrette, R.R. II; Sathisan, S.K.; di Bartolo, R. [Nevada Univ., Las Vegas, NV (United States). Transportation Research Center

    1991-05-31

    This report presents appendices related to the preliminary assessment and risk analysis for high-level radioactive waste transportation routes to the proposed Yucca Mountain Project repository. Information includes data on population density, traffic volume, ecologically sensitive areas, and accident history.

  16. Waste Feed Delivery System Phase 1 Preliminary Reliability and Availability and Maintainability Analysis [SEC 1 and 2

    Energy Technology Data Exchange (ETDEWEB)

    CARLSON, A.B.

    1999-11-11

    The document presents updated results of the preliminary reliability, availability, maintainability analysis performed for delivery of waste feed from tanks 241-AZ-101 and 241-AN-105 to British Nuclear Fuels Limited, inc. under the Tank Waste Remediation System Privatization Contract. The operational schedule delay risk is estimated and contributing factors are discussed.

  17. A preliminary assessment of using a white light confocal imaging profiler for cut mark analysis.

    Science.gov (United States)

    Schmidt, Christopher W; Moore, Christopher R; Leifheit, Randell

    2012-01-01

    White light confocal microscopy creates detailed 3D representations of microsurfaces that can be qualitatively and quantitatively analyzed. The study describes its application to the analysis of cut marks on bone, particularly when discerning cuts made by steel tools from those made by stone. The process described comes from a study where cuts were manually made on a cow rib with seven cutting tools, four stone (an unmodified chert flake, a chert biface, a bifacially ground slate fragment, and an unsharpened piece of slate), and three steel (a Swiss Army Knife, a serrate steak knife, and a serrate saw). Kerfs were magnified ×20 and 3D data clouds were generated using a Sensofar(®) White Light Confocal Profiler (WLCP). Kerf profiles and surface areas, volumes, mean depths, and maximum depths were calculated with proprietary software (SensoScan(®) and SolarMap(®)). For the most part, the stone tools make shallower and wider cuts. Kerf floors can be studied at higher magnifications; they were viewed at ×100. When comparing the kerf floors of the unsharpened slate and the serrate steak knife it was found that the slate floor was more uneven, but the serrate steak knife generated more overall relief. Although preliminary, the approach described here successfully distinguishes stone and steel tools; the authors conclude that the WLCP is a promising technology for cut mark analysis because of the very detailed 3D representations it creates and the numerous avenues of analysis it provides.

  18. Preliminary Drop Time Analysis of a Control Rod Using CFD Code

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Myoung Hwan; Park, Jin Seok; Lee, Won Jae [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Park, Jun Hong [SEST Co., Seoul (Korea, Republic of)

    2010-05-15

    A control rod drive mechanism (CRDM) is a reactor regulating system, which can insert and withdraw a control rod containing a neutron absorbing material to control the reactivity of the reactor core. The latch type CRDM for the SMART (System-integrated Modular Advanced ReacTor) is going to be used. The drop time of the control rod in the design stage is one of important parameters for a safety analysis of the reactor. When the control rod is falling down into the core, it is retarded by various forces acting on it such as fluid resistance buoyancy and mechanical friction caused by contacting the inner surface of the guide thimble, etc.. However, complicated coupling of the various forces makes it difficult to predict the drop behavior. This paper describes the development of the 3D CFD analysis model using a FLUENT code. The single control rod of the Westinghouse 17x17 type optimized fuel assembly (W-OFA) was considered for the verification of the CFD model. A preliminary drop time analysis for the SMART with the simulated control rod was performed

  19. A kinetic analysis of manual wheelchair propulsion during start-up on select indoor and outdoor surfaces

    NARCIS (Netherlands)

    Koontz, AM; Cooper, RA; Boninger, ML; Yang, YS; Impink, BG; van der Woude, LHV

    2005-01-01

    The objective of this study was to conduct a kinetic analysis of manual wheelchair propulsion during start-LIP on select indoor and Outdoor surfaces. Eleven manual wheelchairs were fitted with a SMART(Wheel) and their users were asked to Push on a Course consisting of high- and low-pile carpet, indo

  20. Transient kinetic analysis of the catalytic mechanism of haloalkane dehalogenase : Identification of the rate-determining step

    NARCIS (Netherlands)

    Schanstra, JP; Kingma, Jacob; Janssen, DB; Ornstein, RL

    1997-01-01

    We have studied the rate-determining step(s) in the conversion (k(cat)) of 1,2-dibromoethane by haloalkane dehalogenase, a bacterial enzyme involved in degradation of halogenated aliphatic compounds. Analysis of solvent kinetic isotope effects and stopped-flow fluorescence experiments with enzyme

  1. A Preliminary Analysis of Precipitation Properties and Processes during NASA GPM IFloodS

    Science.gov (United States)

    Carey, Lawrence; Gatlin, Patrick; Petersen, Walt; Wingo, Matt; Lang, Timothy; Wolff, Dave

    2014-01-01

    The Iowa Flood Studies (IFloodS) is a NASA Global Precipitation Measurement (GPM) ground measurement campaign, which took place in eastern Iowa from May 1 to June 15, 2013. The goals of the field campaign were to collect detailed measurements of surface precipitation using ground instruments and advanced weather radars while simultaneously collecting data from satellites passing overhead. Data collected by the radars and other ground instruments, such as disdrometers and rain gauges, will be used to characterize precipitation properties throughout the vertical column, including the precipitation type (e.g., rain, graupel, hail, aggregates, ice crystals), precipitation amounts (e.g., rain rate), and the size and shape of raindrops. The impact of physical processes, such as aggregation, melting, breakup and coalescence on the measured liquid and ice precipitation properties will be investigated. These ground observations will ultimately be used to improve rainfall estimates from satellites and in particular the algorithms that interpret raw data for the upcoming GPM mission's Core Observatory satellite, which launches in 2014. The various precipitation data collected will eventually be used as input to flood forecasting models in an effort to improve capabilities and test the utility and limitations of satellite precipitation data for flood forecasting. In this preliminary study, the focus will be on analysis of NASA NPOL (S-band, polarimetric) radar (e.g., radar reflectivity, differential reflectivity, differential phase, correlation coefficient) and NASA 2D Video Disdrometers (2DVDs) measurements. Quality control and processing of the radar and disdrometer data sets will be outlined. In analyzing preliminary cases, particular emphasis will be placed on 1) documenting the evolution of the rain drop size distribution (DSD) as a function of column melting processes and 2) assessing the impact of range on ground-based polarimetric radar estimates of DSD properties.

  2. Evolution of inhibitor-resistant natural mutant forms of HIV-1 protease probed by pre-steady state kinetic analysis.

    Science.gov (United States)

    Zakharova, Maria Yu; Kuznetsova, Alexandra A; Kaliberda, Elena N; Dronina, Maria A; Kolesnikov, Alexander V; Kozyr, Arina V; Smirnov, Ivan V; Rumsh, Lev D; Fedorova, Olga S; Knorre, Dmitry G; Gabibov, Alexander G; Kuznetsov, Nikita A

    2017-08-23

    Pre-steady state kinetic analysis of mechanistic features of substrate binding and processing is crucial for insight into the evolution of inhibitor-resistant forms of HIV-1 protease. These data may provide a correct vector for rational drug design assuming possible intrinsic dynamic effects. These data should also give some clues to the molecular mechanism of protease action and resistance to inhibitors. Here we report pre-steady state kinetics of the interaction of wild type or mutant forms of HIV-1 protease with a FRET-labeled peptide. The three-stage "minimal" kinetic scheme with first and second reversible steps of substrate binding and with following irreversible peptide cleavage step adequately described experimental data. For the first time, a set of "elementary" kinetic parameters of wild type HIV-1 protease and its natural mutant inhibitor-resistant forms MDR-HM, ANAM-11 and prDRV4 were compared. Inhibitors of the first and second generation were used to estimate the inhibitory effects on HIV-1 protease activity. The resulting set of kinetic data supported that the mutant forms are kinetically unaffected by inhibitors of the first generation, proving their functional resistance to these compounds. The second generation inhibitor darunavir inhibited mutant forms MDR-HM and ANAM-11, but was ineffective against prDRV4. Our kinetic data revealed that these inhibitors induced different conformational changes in the enzyme and, thereby they have different mode of binding in the enzyme active site. These data confirmed hypothesis that the driving force of the inhibitor-resistance evolution is disruption of enzyme-inhibitor complex by changing of the contact network in the inhibitor binding site. Copyright © 2017 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

  3. Comparative Kinetic Analysis of Closed-Ended and Open-Ended Porous Sensors

    Science.gov (United States)

    Zhao, Yiliang; Gaur, Girija; Mernaugh, Raymond L.; Laibinis, Paul E.; Weiss, Sharon M.

    2016-09-01

    Efficient mass transport through porous networks is essential for achieving rapid response times in sensing applications utilizing porous materials. In this work, we show that open-ended porous membranes can overcome diffusion challenges experienced by closed-ended porous materials in a microfluidic environment. A theoretical model including both transport and reaction kinetics is employed to study the influence of flow velocity, bulk analyte concentration, analyte diffusivity, and adsorption rate on the performance of open-ended and closed-ended porous sensors integrated with flow cells. The analysis shows that open-ended pores enable analyte flow through the pores and greatly reduce the response time and analyte consumption for detecting large molecules with slow diffusivities compared with closed-ended pores for which analytes largely flow over the pores. Experimental confirmation of the results was carried out with open- and closed-ended porous silicon (PSi) microcavities fabricated in flow-through and flow-over sensor configurations, respectively. The adsorption behavior of small analytes onto the inner surfaces of closed-ended and open-ended PSi membrane microcavities was similar. However, for large analytes, PSi membranes in a flow-through scheme showed significant improvement in response times due to more efficient convective transport of analytes. The experimental results and theoretical analysis provide quantitative estimates of the benefits offered by open-ended porous membranes for different analyte systems.

  4. In vitro kinetic analysis of oligofructose consumption by Bacteroides and Bifidobacterium spp. indicates different degradation mechanisms.

    Science.gov (United States)

    Van der Meulen, Roel; Makras, Lefteris; Verbrugghe, Kristof; Adriany, Tom; De Vuyst, Luc

    2006-02-01

    The growth of pure cultures of Bacteroides thetaiotaomicron LMG 11262 and Bacteroides fragilis LMG 10263 on fructose and oligofructose was examined and compared to that of Bifidobacterium longum BB536 through in vitro laboratory fermentations. Gas chromatography (GC) analysis was used to determine the different fractions of oligofructose and their degradation during the fermentation process. Both B. thetaiotaomicron LMG 11262 and B. fragilis LMG 10263 were able to grow on oligofructose as fast as on fructose, succinic acid being the major metabolite produced by both strains. B. longum BB536 grew slower on oligofructose than on fructose. Acetic acid and lactic acid were the main metabolites produced when fructose was used as the sole energy source. Increased amounts of formic acid and ethanol were produced when oligofructose was used as an energy source at the cost of lactic acid. Detailed kinetic analysis revealed a preferential metabolism of the short oligofructose fractions (e.g., F2 and F3) for B. longum BB536. After depletion of the short fractions, the larger oligofructose fractions (e.g., F4, GF4, F5, GF5, and F6) were metabolized, too. Both Bacteroides strains did not display such a preferential metabolism and degraded all oligofructose fractions simultaneously, transiently increasing the fructose concentration in the medium. This suggests a different mechanism for oligofructose breakdown between the strain of Bifidobacterium and both strains of Bacteroides, which helps to explain the bifidogenic nature of inulin-type fructans.

  5. Crystallization Kinetics of a Solid Oxide Fuel Cell Seal Glass by Differential Thermal Analysis

    Science.gov (United States)

    Bansal, Narottam P.; Gamble, Eleanor A.

    2005-01-01

    Crystallization kinetics of a barium calcium aluminosilicate glass (BCAS), a sealant material for planar solid oxide fuel cells, have been investigated by differential thermal analysis (DTA). From variation of DTA peak maximum temperature with heating rate, the activation energy for glass crystallization was calculated to be 259 kJ/mol. Development of crystalline phases on thermal treatments of the glass at various temperatures has been followed by powder x-ray diffraction. Microstructure and chemical composition of the crystalline phases were investigated by scanning electron microscopy and energy dispersive spectroscopic (EDS) analysis. BaSiO3 and hexacelsian (BaAl2Si2O8) were the primary crystalline phases whereas monoclinic celsian (BaAl2Si2O8) and (Ba(x), Ca(y))SiO4 were also detected as minor phases. Needle-shaped BaSiO3 crystals are formed first, followed by the formation of other phases at longer times of heat treatments. The glass does not fully crystallize even after long term heat treatments at 750 to 900 C.

  6. Novel degradation pathway and kinetic analysis for buprofezin removal by newly isolated Bacillus sp.

    Science.gov (United States)

    Wang, Guangli; Xu, Dayong; Xiong, Minghua; Zhang, Hui; Li, Feng; Liu, Yuan

    2016-09-15

    Given the intensive and widespread application of the pesticide, buprofezin, its environmental residues potentially pose a problem; yet little is known about buprofezin's kinetic and metabolic behaviors. In this study, a novel gram-positive strain, designated BF-5, isolated from aerobic activated sludge, was found to be capable of metabolizing buprofezin as its sole energy, carbon, and nitrogen source. Based on its physiological and biochemical characteristics, other aspects of its phenotype, and a phylogenetic analysis, strain BF-5 was identified as Bacillus sp. This study investigated the effect of culture conditions on bacterial growth and substrate degradation, such as pH, temperature, initial concentration, different nitrogen source, and additional nitrogen sources as co-substrates. The degradation rate parameters, qmax, Ks, Ki and Sm were determined to be 0.6918 h(-1), 105.4 mg L(-1), 210.5 mg L(-1), and 148.95 mg L(-1) respectively. The capture of unpublished potential metabolites by gas chromatography-mass spectrometry (GC-MS) analysis has led to the proposal of a novel degradation pathway. Taken together, our results clarify buprofezin's biodegradation pathway(s) and highlight the promising potential of strain BF-5 in bioremediation of buprofezin-contaminated environments. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Thermal analysis and kinetics of coal during oxy-fuel combustion

    Science.gov (United States)

    Kosowska-Golachowska, Monika

    2017-08-01

    The pyrolysis and oxy-fuel combustion characteristics of Polish bituminous coal were studied using non-isothermal thermogravimetric analysis. Pyrolysis tests showed that the mass loss profiles were almost similar up to 870°C in both N2 and CO2 atmospheres, while further mass loss occurred in CO2 atmosphere at higher temperatures due to char-CO2 gasification. Replacement of N2 in the combustion environment by CO2 delayed the combustion of bituminous coal. At elevated oxygen levels, TG/DTG profiles shifted through lower temperature zone, ignition and burnout temperatures decreased and mass loss rate significantly increased and complete combustion was achieved at lower temperatures and shorter times. Kinetic analysis for the tested coal was performed using Kissinger-Akahira-Sunose (KAS) method. The activation energies of bituminous coal combustion at the similar oxygen content in oxy-fuel with that of air were higher than that in air atmosphere. The results indicated that, with O2 concentration increasing, the activation energies decreased.

  8. Anaerobic digestion of linear alkyl benzene sulfonates: biodegradation kinetics and metabolite analysis.

    Science.gov (United States)

    García, M T; Campos, E; Ribosa, I; Latorre, A; Sánchez-Leal, J

    2005-09-01

    In the present work the effect of the alkyl chain length and the position of the sulfophenyl substituent of the linear alkylbenzene sulfonates (LAS) on their anaerobic biodegradability have been investigated. Degradation kinetics of the linear alkyl benzene sulfonates homologues, 2phiC10LAS, 2phiC12LAS and 2phiC14LAS, have been studied. It has been also investigated the effect of the isomer type on the degradation rate of the LAS molecule through the comparative study of the 2phiC10LAS and 5phiC10LAS isomers. Batch anaerobic biodegradation tests were performed using sludge from the anaerobic digester of a wastewater treatment plant as microorganisms source. Ultimate biodegradation was evaluated from the biogas production whereas primary biodegradation was determined by specific analysis of the surfactant. LAS homologues and isomers showed a negligible primary biodegradation under anaerobic conditions. Furthermore, analysis of sulfophenyl carboxilates (SPC) by LC-MS indicated a low and constant level of these LAS degradation metabolites over the test period. These data are consistent with a minimal transformation of the LAS parent molecule in the anaerobic digesters. On the other hand, the addition of the shortest alkyl chain length homologues, decyl and dodecylbenzene sulfonates, reduces the biogas production whereas the most hydrophobic homologue, the tetradecylbenzene sulfonate, enhances the biogas production. This LAS homologue seems to increase the availability of organic compounds sorbed on the anaerobic sludge promoting their biodegradation.

  9. Combustion behavior and kinetics of low-lipid microalgae via thermogravimetric analysis.

    Science.gov (United States)

    Gai, Chao; Liu, Zhengang; Han, Guanghua; Peng, Nana; Fan, Aonan

    2015-04-01

    Thermogravimetric analysis and differential thermal analysis were employed to investigate combustion characteristics of two low-lipid microalgae, Chlorella pyrenoidosa (CP) and Spirulina platensis (SP) and iso-conversional Starink approach was used to calculate the kinetic parameters in the present study. The results showed that three stages of mass loss, including dehydration, devolatilization and char oxidation, were observed during combustion of both of two low-lipid microalgae. The whole weight loss of combustion of two microalgae was both shifted to higher temperature zones with increased heating rates from 10 to 40 K/min. In the 0.1-0.9 conversion range, the apparent activation energy of CP increased first from 51.96 to 79.53 kJ/mol, then decreased to 55.59 kJ/mol. Finally, it slightly increased to 67.27 kJ/mol. In the case of SP, the apparent activation energy gradually increased from 68.51 to 91.06 kJ/mol.

  10. The platinum microelectrode/Nafion interface - An electrochemical impedance spectroscopic analysis of oxygen reduction kinetics and Nafion characteristics

    Science.gov (United States)

    Parthasarathy, Arvind; Dave, Bhasker; Srinivasan, Supramaniam; Appleby, John A.; Martin, Charles R.

    1992-01-01

    The objectives of this study were to use electrochemical impedance spectroscopy (EIS) to study the oxygen-reduction reaction under lower humidification conditions than previously studied. The EIS technique permits the discrimination of electrode kinetics of oxygen reduction, mass transport of O2 in the membrane, and the electrical characteristics of the membrane. Electrode-kinetic parameters for the oxygen-reduction reaction, corrosion current densities for Pt, and double-layer capacitances were calculated. The production of water due to electrochemical reduction of oxygen greatly influenced the EIS response and the electrode kinetics at the Pt/Nafion interface. From the finite-length Warburg behavior, a measure of the diffusion coefficient of oxygen in Nafion and diffusion-layer thickness was obtained. An analysis of the EIS data in the high-frequency domain yielded membrane and interfacial characteristics such as ionic conductivity of the membrane, membrane grain-boundary capacitance and resistance, and uncompensated resistance.

  11. Entropy Analysis of Kinetic Flux Vector Splitting Schemes for the Compressible Euler Equations

    Science.gov (United States)

    Shiuhong, Lui; Xu, Jun

    1999-01-01

    Flux Vector Splitting (FVS) scheme is one group of approximate Riemann solvers for the compressible Euler equations. In this paper, the discretized entropy condition of the Kinetic Flux Vector Splitting (KFVS) scheme based on the gas-kinetic theory is proved. The proof of the entropy condition involves the entropy definition difference between the distinguishable and indistinguishable particles.

  12. Machine vision analysis for industrial beet color change kinetics and total soluble solid content

    Science.gov (United States)

    A machine vision system (MVS) for the measurement of color change kinetics in crushed industrial beet to evaluate the total soluble solid content (°Brix) was developed in this study. It is expected that higher the °Brix faster the color change and modeling this color change kinetics helps in assessi...

  13. Preliminary Thermohydraulic Analysis of a New Moderated Reactor Utilizing an LEU-Fuel for Space Nuclear Thermal Propulsion

    Energy Technology Data Exchange (ETDEWEB)

    Nam, Seung Hyun; Choi, Jae Young; Venneria, Paolo F.; Jeong, Yong Hoon; Chang, Soon Heung [KAIST, Daejeon (Korea, Republic of)

    2015-10-15

    The Korea Advanced NUclear Thermal Engine Rocket utilizing an LEU fuel (KANUTER-LEU) is a non-proliferative and comparably efficient NTR engine with relatively low thrust levels of 40 - 50 kN for in-space transportation. The small modular engine can expand mission versatility, when flexibly used in a clustered engine arrangement, so that it can perform various scale missions from low-thrust robotic science missions to high-thrust manned missions. In addition, the clustered engine system can enhance engine redundancy and ensuing crew safety as well as the thrust. The propulsion system is an energy conversion system to transform the thermal energy of the reactor into the kinetic energy of the propellant to produce the powers for thrust, propellant feeding and electricity. It is mainly made up of a propellant Feeding System (PFS) comprising a Turbo-Pump Assembly (TPA), a Regenerative Nozzle Assembly (RNA), etc. For this core design study, an expander cycle is assumed to be the propulsion system. The EGS converts the thermal energy of the EHTGR in the idle operation (only 350 kW{sub th} power) to electric power during the electric power mode. This paper presents a preliminary thermohydraulic design analysis to explore the design space for the new reactor and to estimate the referential engine performance. The new non-proliferative NTR engine concept, KANUTER-LEU, is under designing to surmount the nuclear proliferation obstacles on allR and Dactivities and eventual commercialization for future generations. To efficiently implement a heavy LEU fuel for the NTR engine, its reactor design innovatively possesses the key characteristics of the high U density fuel with high heating and H{sub 2} corrosion resistances, the thermal neutron spectrum core and also minimizing non-fission neutron loss, and the compact reactor design with protectively cooling capability. To investigate feasible design space for the moderated EHTGR-LEU and resultant engine performance, the

  14. Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes

    Directory of Open Access Journals (Sweden)

    Jan David Kijlstra

    2015-12-01

    Full Text Available The quantitative analysis of cardiomyocyte function is essential for stem cell-based approaches for the in vitro study of human cardiac physiology and pathophysiology. We present a method to comprehensively assess the function of single human pluripotent stem cell-derived cardiomyocyte (hPSC-CMs through simultaneous quantitative analysis of contraction kinetics, force generation, and electrical activity. We demonstrate that statistical analysis of movies of contracting hPSC-CMs can be used to quantify changes in cellular morphology over time and compute contractile kinetics. Using a biomechanical model that incorporates substrate stiffness, we calculate cardiomyocyte force generation at single-cell resolution and validate this approach with conventional traction force microscopy. The addition of fluorescent calcium indicators or membrane potential dyes allows the simultaneous analysis of contractility and calcium handling or action potential morphology. Accordingly, our approach has the potential for broad application in the study of cardiac disease, drug discovery, and cardiotoxicity screening.

  15. CERENA: ChEmical REaction Network Analyzer--A Toolbox for the Simulation and Analysis of Stochastic Chemical Kinetics.

    Science.gov (United States)

    Kazeroonian, Atefeh; Fröhlich, Fabian; Raue, Andreas; Theis, Fabian J; Hasenauer, Jan

    2016-01-01

    Gene expression, signal transduction and many other cellular processes are subject to stochastic fluctuations. The analysis of these stochastic chemical kinetics is important for understanding cell-to-cell variability and its functional implications, but it is also challenging. A multitude of exact and approximate descriptions of stochastic chemical kinetics have been developed, however, tools to automatically generate the descriptions and compare their accuracy and computational efficiency are missing. In this manuscript we introduced CERENA, a toolbox for the analysis of stochastic chemical kinetics using Approximations of the Chemical Master Equation solution statistics. CERENA implements stochastic simulation algorithms and the finite state projection for microscopic descriptions of processes, the system size expansion and moment equations for meso- and macroscopic descriptions, as well as the novel conditional moment equations for a hybrid description. This unique collection of descriptions in a single toolbox facilitates the selection of appropriate modeling approaches. Unlike other software packages, the implementation of CERENA is completely general and allows, e.g., for time-dependent propensities and non-mass action kinetics. By providing SBML import, symbolic model generation and simulation using MEX-files, CERENA is user-friendly and computationally efficient. The availability of forward and adjoint sensitivity analyses allows for further studies such as parameter estimation and uncertainty analysis. The MATLAB code implementing CERENA is freely available from http://cerenadevelopers.github.io/CERENA/.

  16. Preliminary analysis of Stearoyl Co-A Desaturase gene transcripts in River buffalo

    Directory of Open Access Journals (Sweden)

    L. Ramunno

    2010-02-01

    Full Text Available Stearoyl-CoA desaturase (SCD is a key enzyme in the biosynthesis of monounsaturated fatty acids (MUFAs. In cattle, SCD gene extends over a DNA segment of ~17.0 Kb, and it is organized in 6 exons and 5 introns. The SCD gene has been indicated as the candidate gene to change the saturated/unsaturated FAs ratio and hence it has been suggested as the gene influencing the fat quality. In cattle, eight SNPs have been identified and one of them, (T→C at 231st nt of 5th exon, is responsible for the Val→Ala amino acid change. The C allele has been associated with higher content of MUFAs in carcasses, and it is positively related to a higher index of desaturation (C18:0/C18:1 and C16:0/C16:1 in the milk. In this study, we report on preliminary results of analysis of transcripts of the SCD encoding gene in river buffalo. The electrophoretic analysis of the RT-PCR products and the subsequent sequencing showed at least five different populations of mRNA. The most represented population is correctly assembled (~1300 bp, followed by the one which is deleted of ~750bp, corresponding to the 3rd, 4th and 5th exon and partially to the 2nd and 6th exon.

  17. Biomarkers of Eating Disorders Using Support Vector Machine Analysis of Structural Neuroimaging Data: Preliminary Results.

    Science.gov (United States)

    Cerasa, Antonio; Castiglioni, Isabella; Salvatore, Christian; Funaro, Angela; Martino, Iolanda; Alfano, Stefania; Donzuso, Giulia; Perrotta, Paolo; Gioia, Maria Cecilia; Gilardi, Maria Carla; Quattrone, Aldo

    2015-01-01

    Presently, there are no valid biomarkers to identify individuals with eating disorders (ED). The aim of this work was to assess the feasibility of a machine learning method for extracting reliable neuroimaging features allowing individual categorization of patients with ED. Support Vector Machine (SVM) technique, combined with a pattern recognition method, was employed utilizing structural magnetic resonance images. Seventeen females with ED (six with diagnosis of anorexia nervosa and 11 with bulimia nervosa) were compared against 17 body mass index-matched healthy controls (HC). Machine learning allowed individual diagnosis of ED versus HC with an Accuracy ≥ 0.80. Voxel-based pattern recognition analysis demonstrated that voxels influencing the classification Accuracy involved the occipital cortex, the posterior cerebellar lobule, precuneus, sensorimotor/premotor cortices, and the medial prefrontal cortex, all critical regions known to be strongly involved in the pathophysiological mechanisms of ED. Although these findings should be considered preliminary given the small size investigated, SVM analysis highlights the role of well-known brain regions as possible biomarkers to distinguish ED from HC at an individual level, thus encouraging the translational implementation of this new multivariate approach in the clinical practice.

  18. Biomarkers of Eating Disorders Using Support Vector Machine Analysis of Structural Neuroimaging Data: Preliminary Results

    Directory of Open Access Journals (Sweden)

    Antonio Cerasa

    2015-01-01

    Full Text Available Presently, there are no valid biomarkers to identify individuals with eating disorders (ED. The aim of this work was to assess the feasibility of a machine learning method for extracting reliable neuroimaging features allowing individual categorization of patients with ED. Support Vector Machine (SVM technique, combined with a pattern recognition method, was employed utilizing structural magnetic resonance images. Seventeen females with ED (six with diagnosis of anorexia nervosa and 11 with bulimia nervosa were compared against 17 body mass index-matched healthy controls (HC. Machine learning allowed individual diagnosis of ED versus HC with an Accuracy ≥ 0.80. Voxel-based pattern recognition analysis demonstrated that voxels influencing the classification Accuracy involved the occipital cortex, the posterior cerebellar lobule, precuneus, sensorimotor/premotor cortices, and the medial prefrontal cortex, all critical regions known to be strongly involved in the pathophysiological mechanisms of ED. Although these findings should be considered preliminary given the small size investigated, SVM analysis highlights the role of well-known brain regions as possible biomarkers to distinguish ED from HC at an individual level, thus encouraging the translational implementation of this new multivariate approach in the clinical practice.

  19. The 6 April 2009 earthquake at L'Aquila: a preliminary analysis of magnetic field measurements

    Directory of Open Access Journals (Sweden)

    U. Villante

    2010-02-01

    Full Text Available Several investigations reported the possible identification of anomalous geomagnetic field signals prior to earthquake occurrence. In the ULF frequency range, candidates for precursory signatures have been proposed in the increase in the noise background and polarization parameter (i.e. the ratio between the amplitude/power of the vertical component and that one of the horizontal component, in the changing characteristics of the slope of the power spectrum and fractal dimension, in the possible occurrence of short duration pulses. We conducted, with conventional techniques of data processing, a preliminary analysis of the magnetic field observations performed at L'Aquila during three months preceding the 6 April 2009 earthquake, focusing attention on the possible occurrence of features similar to those identified in previous events. Within the limits of this analysis, we do not find compelling evidence for any of the features which have been proposed as earthquake precursors: indeed, most of aspects of our observations (which, in some cases, appear consistent with previous findings might be interpreted in terms of the general magnetospheric conditions and/or of different sources.

  20. Crystallization and preliminary X-ray diffraction analysis of human adenovirus

    Energy Technology Data Exchange (ETDEWEB)

    Reddy, V.S.; Natchiar, S.K.; Gritton, L.; Mullen, T.-M.; Stewart, P.L.; Nemerow, G.R. (Scripps); (Vanderbilt)

    2010-07-22

    Replication-defective and conditionally replicating adenovirus (AdV) vectors are currently being utilized in {approx}25% of human gene transfer clinical trials. Unfortunately, progress in vector development has been hindered by a lack of accurate structural information. Here we describe the crystallization and preliminary X-ray diffraction analysis of a HAdV5 vector that displays a short flexible fiber derived from HAdV35. Crystals of Ad35F were grown in 100 mM HEPES pH 7.0, 200 mM Ca(OAc){sub 2}, 14% PEG 550 MME, 15% glycerol in 100 mM Tris-HCl 8.5. Freshly grown crystals diffracted well to 4.5 {angstrom} resolution and weakly to 3.5 {angstrom} at synchrotron sources. HAdV crystals belong to space group P1 with unit cell parameters a = 854.03 {angstrom}, b = 855.17 {angstrom}, c = 865.24 {angstrom}, {alpha} = 119.57{sup o}, {beta} = 91.71{sup o}, {gamma} = 118.08{sup o} with a single particle in the unit cell. Self-rotation and locked-rotation function analysis allowed the determination of the particle orientation. Molecular replacement, density modification and phase-extension procedures are being employed for structure determination.

  1. Ocean thermal energy conversion cold water pipe preliminary design project. Task 2. Analysis for concept selection

    Energy Technology Data Exchange (ETDEWEB)

    None

    1979-04-01

    The successful performance of the CWP is of crucial importance to the overall OTEC system; the pipe itself is considered the most critical part of the entire operation. Because of the importance the CWP, a project for the analysis and design of CWP's was begun in the fall of 1978. The goals of this project were to study a variety of concepts for delivering cold water to an OTEC plant, to analyze and rank these concepts based on their relative cost and risk, and to develop preliminary design for those concepts which seemed most promising. Two representative platforms and sites were chosen: a spar buoy of a Gibbs and Cox design to be moored at a site off Punta Tuna, Puerto Rico, and a barge designed by APL/Johns Hopkins University, grazing about a site approximately 200 miles east of the coast of Brazil. The approach was to concentrate on the most promising concepts and on those which were either of general interest or espoused by others (e.g., steel and concrete concepts). Much of the overall attention, therefore, focused on analyzing rigid and compliant wall design, while stockade (except for the special case of the FRP stockade) and bottom-mounted concepts received less attention. A total of 67 CWP concepts were initially generated and subjected to a screening process. Of these, 16 were carried through design analysis, costing, and ranking. Study results are presented in detail. (WHK)

  2. A method for analysis and design of metabolism using metabolomics data and kinetic models: Application on lipidomics using a novel kinetic model of sphingolipid metabolism.

    Science.gov (United States)

    Savoglidis, Georgios; da Silveira Dos Santos, Aline Xavier; Riezman, Isabelle; Angelino, Paolo; Riezman, Howard; Hatzimanikatis, Vassily

    2016-09-01

    We present a model-based method, designated Inverse Metabolic Control Analysis (IMCA), which can be used in conjunction with classical Metabolic Control Analysis for the analysis and design of cellular metabolism. We demonstrate the capabilities of the method by first developing a comprehensively curated kinetic model of sphingolipid biosynthesis in the yeast Saccharomyces cerevisiae. Next we apply IMCA using the model and integrating lipidomics data. The combinatorial complexity of the synthesis of sphingolipid molecules, along with the operational complexity of the participating enzymes of the pathway, presents an excellent case study for testing the capabilities of the IMCA. The exceptional agreement of the predictions of the method with genome-wide data highlights the importance and value of a comprehensive and consistent engineering approach for the development of such methods and models. Based on the analysis, we identified the class of enzymes regulating the distribution of sphingolipids among species and hydroxylation states, with the D-phospholipase SPO14 being one of the most prominent. The method and the applications presented here can be used for a broader, model-based inverse metabolic engineering approach.

  3. Kinetic and economic analysis of reactive capture of dilute carbon dioxide with Grignard reagents.

    Science.gov (United States)

    Dowson, G R M; Dimitriou, I; Owen, R E; Reed, D G; Allen, R W K; Styring, P

    2015-01-01

    Carbon Dioxide Utilisation (CDU) processes face significant challenges, especially in the energetic cost of carbon capture from flue gas and the uphill energy gradient for CO2 reduction. Both of these stumbling blocks can be addressed by using alkaline earth metal compounds, such as Grignard reagents, as sacrificial capture agents. We have investigated the performance of these reagents in their ability to both capture and activate CO2 directly from dried flue gas (essentially avoiding the costly capture process entirely) at room temperature and ambient pressures with high yield and selectivity. Naturally, to make the process sustainable, these reagents must then be recycled and regenerated. This would potentially be carried out using existing industrial processes and renewable electricity. This offers the possibility of creating a closed loop system whereby alcohols and certain hydrocarbons may be carboxylated with CO2 and renewable electricity to create higher-value products containing captured carbon. A preliminary Techno-Economic Analysis (TEA) of an example looped process has been carried out to identify the electrical and raw material supply demands and hence determine production costs. These have compared broadly favourably with existing market values.

  4. Reactive Aggression and Suicide-Related Behaviors in Children and Adolescents: A Review and Preliminary Meta-Analysis.

    Science.gov (United States)

    Hartley, Chelsey M; Pettit, Jeremy W; Castellanos, Daniel

    2017-01-03

    The empirical literature on the association between reactive aggression and suicide-related behaviors in children and adolescents was reviewed. A narrative review of seven studies that met inclusion/exclusion criteria is followed by a preliminary meta-analysis to provide insight into the strength of the association between reactive aggression and suicide-related behaviors. Each of the seven studies reported a statistically significant association between reactive aggression and suicide-related behaviors, including suicide, nonfatal suicide attempt, and suicide ideation. Results from the meta-analysis indicated a consistent, medium-sized association (k = 7; N = 4,693; rbar = .25). The narrative review and results of the preliminary meta-analysis support the promise of pursuing future research on reactive aggression and suicide-related behaviors in children and adolescents. A theoretical model is proposed to guide the development of future research.

  5. Report on preliminary analysis of state of nuclear criticality accident at JCO at Tokaimura, Ibaraki, Japan (I)

    Energy Technology Data Exchange (ETDEWEB)

    Ha, J.J.; Park, J.H.; Chang, J.H. [Korea Atomic Energy Research Institute, Taejon (Korea)

    1999-10-01

    This preliminary report was prepared by the Special Task Force Team of KAERI in order to analysis status of nuclear criticality accident broken out at 10:35 September 30, 1999 at JCO nuclear conversion test facility located at Tokaimura, Ibaraki, Japan. The report was consisted of accident summary of cause of accident summary of cause of accident and response by relevant organizations, and preliminary technical analysis of radiation exposure of JCO workers, analysis of cause of accident, and accident assessment and preventive actions against criticality accident. It is expected that JCO accident, Japan's first nuclear criticality accident, would make significant effects to Japan nuclear policy and would be also a good example to Korea future actions to be taken in use and development of nuclear energy. 63 refs., 3 figs., 1 tab. (Author)

  6. Vacuum pyrolysis characteristics and kinetic analysis of liquid crystal from scrap liquid crystal display panels.

    Science.gov (United States)

    Chen, Ya; Zhang, Lingen; Xu, Zhenming

    2017-04-05

    Recycling of waste liquid crystal display (LCD) panels is an urgent task with the rapid expanding LCD market. However, as important composition of LCD panels, the treatment of liquid crystal is seldom concerned for its low concentration. In present study, a stripping product enriched liquid crystal and indium is gained by mechanical stripping process, in which liquid crystal is enriched from 0.3wt.% to 53wt.% and indium is enriched from 0.02wt.% to 7.95wt.%. For the stripping product, liquid crystal should be removed before indium recovery because (a) liquid crystal will hinder indium recycling; (b) liquid crystal is hazardous waste. Hence, an effective and green approach by vacuum pyrolysis is proposed to treat liquid crystal in the stripping product. The results are summarized as: (i) From the perspective of apparent activation energy, the advantages of vacuum pyrolysis is expounded according to kinetic analysis. (ii) 89.10wt.% of liquid crystal is converted and the content of indium in residue reaches 14.18wt.% under 773K, 15min and system pressure of 20Pa. This study provides reliable information for further industrial application and an essential pretreatment for the next step of indium recycling.

  7. Unscented Kalman filter with parameter identifiability analysis for the estimation of multiple parameters in kinetic models

    Directory of Open Access Journals (Sweden)

    Baker Syed

    2011-01-01

    Full Text Available Abstract In systems biology, experimentally measured parameters are not always available, necessitating the use of computationally based parameter estimation. In order to rely on estimated parameters, it is critical to first determine which parameters can be estimated for a given model and measurement set. This is done with parameter identifiability analysis. A kinetic model of the sucrose accumulation in the sugar cane culm tissue developed by Rohwer et al. was taken as a test case model. What differentiates this approach is the integration of an orthogonal-based local identifiability method into the unscented Kalman filter (UKF, rather than using the more common observability-based method which has inherent limitations. It also introduces a variable step size based on the system uncertainty of the UKF during the sensitivity calculation. This method identified 10 out of 12 parameters as identifiable. These ten parameters were estimated using the UKF, which was run 97 times. Throughout the repetitions the UKF proved to be more consistent than the estimation algorithms used for comparison.

  8. Unscented Kalman filter with parameter identifiability analysis for the estimation of multiple parameters in kinetic models.

    Science.gov (United States)

    Baker, Syed Murtuza; Poskar, C Hart; Junker, Björn H

    2011-10-11

    In systems biology, experimentally measured parameters are not always available, necessitating the use of computationally based parameter estimation. In order to rely on estimated parameters, it is critical to first determine which parameters can be estimated for a given model and measurement set. This is done with parameter identifiability analysis. A kinetic model of the sucrose accumulation in the sugar cane culm tissue developed by Rohwer et al. was taken as a test case model. What differentiates this approach is the integration of an orthogonal-based local identifiability method into the unscented Kalman filter (UKF), rather than using the more common observability-based method which has inherent limitations. It also introduces a variable step size based on the system uncertainty of the UKF during the sensitivity calculation. This method identified 10 out of 12 parameters as identifiable. These ten parameters were estimated using the UKF, which was run 97 times. Throughout the repetitions the UKF proved to be more consistent than the estimation algorithms used for comparison.

  9. Combined Kinetic Studies and Computational Analysis on Kojic Acid Analogs as Tyrosinase Inhibitors

    Directory of Open Access Journals (Sweden)

    Carlyle Ribeiro Lima

    2014-07-01

    Full Text Available Tyrosinase is a key enzyme in melanin synthesis and widely distributed in plants and animals tissues. In mammals, this enzyme is related to pigment production, involved in wound healing, primary immune response and it can also contribute to catecholamines synthesis in the brain. Consequently, tyrosinase enzyme represents an attractive and selective target in the field of the medicine, cosmetics and bio-insecticides. In this paper, experimental kinetics and computational analysis were used to study the inhibition of tyrosinase by analogous of Kojic acid. The main interactions occurring between inhibitors-tyrosinase complexes and the influence of divalent cation (Cu2+ in enzymatic inhibition were investigated by using molecular docking, molecular dynamic simulations and electrostatic binding free energy by using the Linear Interaction Energy (LIE method. The results showed that the electrostatic binding free energy are correlated with values of constant inhibition (r2 = 0.97.Thus, the model obtained here could contribute to future studies of this important system and, therefore, eventually facilitate development of tyrosinase inhibitors.

  10. Kinetic analysis of interaction between N atoms and O-covered Ru(0001)

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Kai, E-mail: kangkai@caep.cn [Center of Interface Dynamics for Sustainability, China Academy of Engineering Physics, Chengdu, Sichuan 610200 (China); Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box 718-35, Mianyang, Sichuan 621907 (China); Kleyn, A. W. [Center of Interface Dynamics for Sustainability, China Academy of Engineering Physics, Chengdu, Sichuan 610200 (China); FOM Institute DIFFER (Dutch Institute For Fundamental Energy Research), P.O. Box 6336, 5600 HH Eindhoven (Netherlands); Gleeson, M. A. [FOM Institute DIFFER (Dutch Institute For Fundamental Energy Research), P.O. Box 6336, 5600 HH Eindhoven (Netherlands)

    2015-10-28

    Eley-Rideal (ER) reactions involving neutral atoms heavier than hydrogen reacting with adsorbed atoms of similar mass were first observed in recent molecular beam experiments by Zaharia et al. [Phys. Rev. Lett. 113, 053201 (2014)]. Through analysis of two types of measurements, they obtained different estimations for the N–O ER reaction cross section, one of which is unexpectedly high. This was qualitatively accounted for by invoking a secondary effect whereby the presence of N adatoms on the surface acted to “shield” O adatoms from prompt recombinative desorption. We apply a rate equation model that includes two ER processes involving different adsorbed species (N–O{sub ad} and N–N{sub ad}) and an N-adsorption process to the full-beam exposure subset of the experimental data in order to study the reaction kinetics. Values for the individual reaction cross sections are derived. The measured N{sub 2} response can be well described by the model, but it is insufficient to completely describe the NO response. Modeling of different exposures is used to evaluate the qualitative picture presented by Zaharia et al.

  11. Qualitative and kinetic analysis of torrefaction of lignocellulosic biomass using DSC-TGA-FTIR

    Directory of Open Access Journals (Sweden)

    Bimal Acharya

    2015-11-01

    Full Text Available Torrefaction is a thermochemical conversion technique to improve the fuel properties of lignocellulosic biomass by treating at temperature 200 ℃-300 ℃ in the minimum oxygen environment for a reasonable residence time. In this study, thermal decomposition and thermal activities of miscanthus and wheat straw during the torrefaction at 200 ℃, 275 ℃, and 300 ℃ in a nitrogen environment for 45 minutes of residence time are analyzed in a simultaneous thermogravimetric analyzer (micro TGA with a differential scanning calorimetry (DSC, and a macro-TGA. The output of the micro TGA is fed into the Fourier transform infrared spectrometry (FTIR and qualitative analysis of the gaseous product is carried out. The composition of different gas products during the torrefaction of biomass are compared critically and kinetics were analyzed. It is found that the weight loss due to degradation of initial biomass in second stage (torrefaction process is a much faster conversion process than the weight loss process in the first stage (drying process. The weight loss of biomass increases with increase in the residence time and torrefaction treatment temperatures. The yield after torrefaction is a solid bio-coal product. The torrefied product were less reactive and has nearly 25% better heating value than the raw biomass. Between the two feedstocks studied, torrefied miscanthus proved to be a more stable fuel than the torrefied wheat straw. The major gaseous components observed during torrefaction are water, carbon dioxide, carbon monoxide, 1,2-Dibromethylene.

  12. Analysis of fluid fuel flow to the neutron kinetics on molten salt reactor FUJI-12

    Energy Technology Data Exchange (ETDEWEB)

    Aji, Indarta Kuncoro, E-mail: indartaaji@s.itb.ac.id [Department of Physics, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesa 10 Bandung 40132 (Indonesia); Waris, Abdul, E-mail: awaris@fi.itb.ac.id; Permana, Sidik [Nuclear Physics & Biophysics Research Division, Department of Physics, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesa 10 Bandung 40132 (Indonesia)

    2015-09-30

    Molten Salt Reactor is a reactor are operating with molten salt fuel flowing. This condition interpret that the neutron kinetics of this reactor is affected by the flow rate of the fuel. This research analyze effect by the alteration velocity of the fuel by MSR type Fuji-12, with fuel composition LiF-BeF{sub 2}-ThF{sub 4}-{sup 233}UF{sub 4} respectively 71.78%-16%-11.86%-0.36%. Calculation process in this study is performed numerically by SOR and finite difference method use C programming language. Data of reactivity, neutron flux, and the macroscopic fission cross section for calculation process obtain from SRAC-CITATION (Standard thermal Reactor Analysis Code) and JENDL-4.0 data library. SRAC system designed and developed by JAEA (Japan Atomic Energy Agency). This study aims to observe the effect of the velocity of fuel salt to the power generated from neutron precursors at fourth year of reactor operate (last critical condition) with number of multiplication effective; 1.0155.

  13. Analysis of fluid fuel flow to the neutron kinetics on molten salt reactor FUJI-12

    Science.gov (United States)

    Aji, Indarta Kuncoro; Waris, Abdul; Permana, Sidik

    2015-09-01

    Molten Salt Reactor is a reactor are operating with molten salt fuel flowing. This condition interpret that the neutron kinetics of this reactor is affected by the flow rate of the fuel. This research analyze effect by the alteration velocity of the fuel by MSR type Fuji-12, with fuel composition LiF-BeF2-ThF4-233UF4 respectively 71.78%-16%-11.86%-0.36%. Calculation process in this study is performed numerically by SOR and finite difference method use C programming language. Data of reactivity, neutron flux, and the macroscopic fission cross section for calculation process obtain from SRAC-CITATION (Standard thermal Reactor Analysis Code) and JENDL-4.0 data library. SRAC system designed and developed by JAEA (Japan Atomic Energy Agency). This study aims to observe the effect of the velocity of fuel salt to the power generated from neutron precursors at fourth year of reactor operate (last critical condition) with number of multiplication effective; 1.0155.

  14. INSTRUMENTATION AND BIOMECHANICAL MODEL FOR KINEMATIC AND KINETIC ANALYSIS OF UPPER LIMBS DURING GAIT WITH CRUTCHES

    Directory of Open Access Journals (Sweden)

    Enrique Pérez-Rizo

    2013-11-01

    Full Text Available The goal of this study was to develop a three-dimensional kinematic and kinetic model of the right upper extremity and a Lofstrand crutch in order to analyze joint displacements and loads during crutch-assisted gait. A Lofstrand crutch was instrumented with a six-component load cell to measure forces and moments at the crutch tip. The crutch and the right upper extremity of a subject were instrumented with markers to obtain kinematic data. A biomechanical model based on rigid bodies was implemented in biomechanical analysis software. To demonstrate the functionality of the model, a pilot test was conducted on one healthy individual during Lofstrand crutch-assisted gait. The shoulder extended during the support phase and flexed in the swing phase, the elbow flexed during the swing, and the wrist remained in extension throughout the cycle. In the shoulder and elbow joints, the predominant reaction forces were upward, whereas the internal force moments were flexion and extension, respectively. This tool will be useful when it comes to identifying risk factors for joint pathology associated with pattern gait, aid design or crutch overuse.

  15. Metabolic engineering of lactic acid bacteria, the combined approach: kinetic modelling, metabolic control and experimental analysis.

    Science.gov (United States)

    Hoefnagel, Marcel H N; Starrenburg, Marjo J C; Martens, Dirk E; Hugenholtz, Jeroen; Kleerebezem, Michiel; Van Swam, Iris I; Bongers, Roger; Westerhoff, Hans V; Snoep, Jacky L

    2002-04-01

    Everyone who has ever tried to radically change metabolic fluxes knows that it is often harder to determine which enzymes have to be modified than it is to actually implement these changes. In the more traditional genetic engineering approaches 'bottle-necks' are pinpointed using qualitative, intuitive approaches, but the alleviation of suspected 'rate-limiting' steps has not often been successful. Here the authors demonstrate that a model of pyruvate distribution in Lactococcus lactis based on enzyme kinetics in combination with metabolic control analysis clearly indicates the key control points in the flux to acetoin and diacetyl, important flavour compounds. The model presented here (available at http://jjj.biochem.sun.ac.za/wcfs.html) showed that the enzymes with the greatest effect on this flux resided outside the acetolactate synthase branch itself. Experiments confirmed the predictions of the model, i.e. knocking out lactate dehydrogenase and overexpressing NADH oxidase increased the flux through the acetolactate synthase branch from 0 to 75% of measured product formation rates.

  16. An investigation on dissolution kinetics of single sodium carbonate particle with image analysis method

    Institute of Scientific and Technical Information of China (English)

    Changdong Li; Carlos Amador; Yulong Ding

    2016-01-01

    Dissolution kinetics of sodium carbonate is investigated with the image analysis method at the approach of single particle. The dissolution experiments are carried out in an aqueous solution under a series of controlled temper-ature and pH. The selected sodium carbonate particles are al spherical with the same mass and diameter. The dissolution process is quantified with the measurement of particle diameter from dissolution images. The concentration of dissolved sodium carbonate in solvent is calculated with the measured diameter of particle. Both surface reaction model and mass transport model are implemented to determine the dissolution mecha-nism and quantify the dissolution rate constant at each experimental condition. According to the fitting results with both two models, it is clarified that the dissolution process at the increasing temperature is controlled by the mass transport of dissolved sodium carbonate travelling from particle surface into solvent. The dissolution process at the increasing pH is control ed by the chemical reaction on particle surface. Furthermore, the dissolution rate constant for each single spherical sodium carbonate particle is quantified and the results show that the disso-lution rate constant of single spherical sodium carbonate increases significantly with the rising of temperature, but decreases with the increasing of pH conversely.

  17. Integrated kinematics-kinetics-plantar pressure data analysis: a useful tool for characterizing diabetic foot biomechanics.

    Science.gov (United States)

    Sawacha, Zimi; Guarneri, Gabriella; Cristoferi, Giuseppe; Guiotto, Annamaria; Avogaro, Angelo; Cobelli, Claudio

    2012-05-01

    The fundamental cause of lower-extremity complications in diabetes is chronic hyperglycemia leading to diabetic foot ulcer pathology. While the relationship between abnormal plantar pressure distribution and plantar ulcers has been widely investigated, little is known about the role of shear stress. Moreover, the mutual relationship among plantar pressure, shear stress, and abnormal kinematics in the etiology of diabetic foot has not been established. This lack of knowledge is determined by the lack of commercially available instruments which allow such a complex analysis. This study aims to develop a method for the simultaneous assessment of kinematics, kinetics, and plantar pressure on foot subareas of diabetic subjects by means of combining three commercial systems. Data were collected during gait on 24 patients (12 controls and 12 diabetic neuropathics) with a motion capture system synchronized with two force plates and two baropodometric systems. A four segment three-dimensional foot kinematics model was adopted for the subsegment angles estimation together with a three segment model for the plantar sub-area definition during gait. The neuropathic group exhibited significantly excessive plantar pressure, ground reaction forces on each direction, and a reduced loading surface on the midfoot subsegment (pfoot ulcerations, and help planning prevention programs.

  18. Characterization and kinetic analysis of enzyme-substrate recognition by three recombinant lactococcal PepVs.

    Science.gov (United States)

    Mori, Sumiko; Miyamoto, Maki; Kaneko, Satoshi; Nirasawa, Satoru; Komba, Shiro; Kasumi, Takafumi

    2006-10-15

    The dipeptidases (PepVs) from three typical lactococcal strains, Lactococcus lactis subsp. lactis (L9), L. lactis subsp. cremoris (L6) and L. lactis subsp. hordniae (hT) were cloned and characterized. The metal-binding, catalytic, and substrate-binding sites are highly conserved among of them. A computer-generated three-dimensional model suggested that the amino acid differences between these PepVs were mostly located away from the active center. L9 PepV does not hydrolyze dipeptides bearing Pro or D-amino acid at the C-terminal amino acid. Unlike PepV from Lactobacillus delbrueckii, L9 PepV does not cleave beta-Asp-His, and has little ability to cleave dipeptides containing a beta-alanine. In addition, L9 PepV has a much higher kcat for dipeptides with an N-terminal Ala but a significantly higher Km when the N-terminal amino acid is Gly. The substrate recognition profile of PepV is further discussed on the basis of the kinetic analysis and the structural model.

  19. Age-Related Differences in Gait Kinematics, Kinetics, and Muscle Function: A Principal Component Analysis.

    Science.gov (United States)

    Schloemer, Sarah A; Thompson, Julie A; Silder, Amy; Thelen, Darryl G; Siston, Robert A

    2017-03-01

    Age-related increased hip extensor recruitment during gait is a proposed compensation strategy for reduced ankle power generation and may indicate a distal-to-proximal shift in muscle function with age. Extending beyond joint level analyses, identifying age-related changes at the muscle level could capture more closely the underlying mechanisms responsible for movement. The purpose of this study was to characterize and compare muscle forces and induced accelerations during gait in healthy older adults with those of young adults. Simulations of one gait cycle for ten older (73.9 ± 5.3 years) and six young (21.0 ± 2.1 years) adults walking at their self-selected speed were analyzed. Muscle force and induced acceleration waveforms, along with kinematic, kinetic, and muscle activation waveforms, were compared between age-groups using principal component analysis. Simulations of healthy older adults had greater gluteus maximus force and vertical support contribution, but smaller iliacus force, psoas force, and psoas vertical support contribution. There were no age-group differences in distal muscle force, contribution, or ankle torque magnitudes. Later peak dorsiflexion and peak ankle angular velocity in older adults may have contributed to their greater ankle power absorption during stance. These findings reveal the complex interplay between age-related changes in neuromuscular control, kinematics, and muscle function during gait.

  20. Biomechanics of octopedal locomotion: kinematic and kinetic analysis of the spider Grammostola mollicoma.

    Science.gov (United States)

    Biancardi, Carlo M; Fabrica, C Gabriel; Polero, Patricia; Loss, Jefferson Fagundes; Minetti, Alberto E

    2011-10-15

    Despite the abundance of octapodal species and their evolutionary importance in originating terrestrial locomotion, the locomotion mechanics of spiders has received little attention so far. In this investigation we use inverse dynamics to study the locomotor performance of Grammostola mollicoma (18 g). Through 3-D kinematic measurements, the trajectory of the eight limbs and cephalothorax or abdomen allowed us to estimate the motion of the body centre of mass (COM) at different speeds. Classic mechanics of locomotion and multivariate analysis of several variables such as stride length and frequency, duty factor, mechanical external work and energy recovery, helped to identify two main gaits, a slow (speed mechanical work (external + internal) calculated in the present study and metabolic data from the literature allowed us to estimate the locomotion efficiency of this species, which was less than 4%. Gait pattern due to alternating limb support, which generates asymmetrical COM trajectories and a small but consistent energy transfer between potential and kinetic energies of COM, is discussed both in terms of coordination indices and by referring to the octopod as formed by two quadrupeds in series. Analogies and differences of the newly obtained parameters with the allometric data and predictions are also illustrated.

  1. Preliminary study: Kinetics of oil extraction from basil (Ocimum basilicum by microwave-assisted hydrodistillation and solvent-free microwave extraction

    Directory of Open Access Journals (Sweden)

    Heri Septya Kusuma

    2016-07-01

    Full Text Available Basil (Ocimum basilicum is an annual herb that is widely grown as a commercial ornamental crop in tropical and warm temperate regions of the world including Asia, Africa, Central and South America. Basil oil is of economic significance due to its widespread utilization in food, cosmetics and pharmaceutical industries. The aim of this study is to investigate the potential of MAHD and SFME for the extraction of essential oils from O. basilicum. In this study, the author also attempted to know and verify the kinetics and mechanism of MAHD and SFME of O. basilicum based on second-order model. In this study, MAHD and SFME are used to extract essential oils from O. basilicum. The initial extraction rate, the extraction capacity and the second-order extraction rate constant were calculated using the model. Kinetics of oil extraction from O. basilicum by MAHD and SFME proved that the extraction process was based on the second-order extraction model as the experimentally done in three different steps. The initial extraction rate, h, was 0.0305 g L−1 min−1 for MAHD and 0.1702 g L−1 min−1 for SFME, the extraction capacity, CS, was 0.8371 g L−1 for MAHD and 0.8229 g L−1 for SFME, the second-order extraction rate constant, k, was 0.0436 L g−1 min−1 for MAHD and 0.2513 L g−1 min−1 for SFME and coefficient of determination, R2, was 0.9355 for MAHD and 0.9842 for SFME.

  2. Kinetic analysis of dynamic 18F-fluoromisonidazole PET correlates with radiation treatment outcome in head-and-neck cancer

    Directory of Open Access Journals (Sweden)

    Paulsen Frank

    2005-12-01

    Full Text Available Abstract Background Hypoxia compromises local control in patients with head-and-neck cancer (HNC. In order to determine the value of [18F]-fluoromisonidazole (Fmiso with regard to tumor hypoxia, a patient study with dynamic Fmiso PET was performed. For a better understanding of tracer uptake and distribution, a kinetic model was developed to analyze dynamic Fmiso PET data. Methods For 15 HNC patients, dynamic Fmiso PET examinations were performed prior to radiotherapy (RT treatment. The data was analyzed using a two compartment model, which allows the determination of characteristic hypoxia and perfusion values. For different parameters, such as patient age, tumor size and standardized uptake value, the correlation to treatment outcome was tested using the Wilcoxon-Mann-Whitney U-test. Statistical tests were also performed for hypoxia and perfusion parameters determined by the kinetic model and for two different metrics based on these parameters. Results The kinetic Fmiso analysis extracts local hypoxia and perfusion characteristics of a tumor tissue. These parameters are independent quantities. In this study, different types of characteristic hypoxia-perfusion patterns in tumors could be identified. The clinical verification of the results, obtained on the basis of the kinetic analysis, showed a high correlation of hypoxia-perfusion patterns and RT treatment outcome (p = 0.001 for this initial patient group. Conclusion The presented study established, that Fmiso PET scans may benefit from dynamic acquisition and analysis by a kinetic model. The pattern of distribution of perfusion and hypoxia in the tissue is correlated to local control in HNC.

  3. Preliminary results on the EUCLID NISP stray-light and ghost analysis

    Science.gov (United States)

    Geis, Norbert; Grupp, Frank; Prieto, Eric; Bender, Ralf

    2015-09-01

    The EUCLID mission within the European Space Agencies 2015 - 2025 Cosmic Vision framework addresses cosmological questions related to dark matter and dark energy. EUCLID is equipped with two instruments that are simultaneously observing patches of > 0:5 square degree on the sky. The VIS visual light high spacial resolution imager and the NISP near infrared spectrometer and photometer are separated by a di-chroic beam splitter. With its large FoV (larger than the full moon disk), together with high demands on the optical performance and strong requirements on in flight stability lead to very challenging demands on alignment and post launch { post cool-down optical element position. In addition the demanding requirements from spectroscopy and galaxy photometry lead to a highly demanding stray light and ghost control need. With this paper we present a preliminary - PDR level - analysis of ghosting and stray light levels in the EUCLID NISP near infrared spectrometer and photometer. The analysis presented focuses on the photometric channel, as this, together with the wide field of the instrument, shows most of the challenges and features of the instrument. As one requirement is to have a non vignetting design, extensive baffling is not possible, and only secondary and higher order light can be actively baffled. A comprehensive ZEMAX based analysis is being presented, showing in summary that baffles are only necessary due to the EUCLID fine guiding sensors auxiliary fields of view. The total level of contaminating light is thereafter dominated by stray light from dust on the lenses. Ghosts play a minor role.

  4. Enantiomeric separation of volatile organics by gas chromatography for the in situ analysis of extraterrestrial materials: kinetics and thermodynamics investigation of various chiral stationary phases.

    Science.gov (United States)

    Freissinet, C; Buch, A; Szopa, C; Sternberg, R

    2013-09-01

    The performances of several commercial chiral capillary columns have been evaluated with the aim of determining the one most suitable for enantiomeric separation in a gas chromatograph onboard a space probe. We compared the GC-MS response of three capillary columns coated with different chiral stationary phases (CSP) using volatile chiral organic molecules which are potential markers of a prebiotic organic chemistry. The three different chiral capillary columns are Chirasil-Val, with an amino acid derivative CSP, ChiralDex-β-PM, with a CSP composed of dissolved permethylated β-cyclodextrins in polysiloxane, and Chirasil-Dex, with a CSP made of modified cyclodextrins chemically bonded to the polysiloxane backbone. Both kinetics and thermodynamics studies have been carried out to evaluate the chiral recognition potential in these different types of columns. The thermodynamic parameters also allow a better understanding of the driving forces affecting the retention and separation of the enantiomers. The Chirasil-Dex-CSP displays the best characteristics for an optimal resolution of the chiral compounds, without preliminary derivatization. This CSP had been chosen to be the only chiral column in the Sample Analysis at Mars (SAM) experiment onboard the current Mars Science Laboratory (MSL) mission, and is also part of the Mars Organic Molecules Analyzer (MOMA) gas chromatograph onboard the next Martian mission ExoMars. The use of this column could also be extended to all space missions aimed at studying chirality in space.

  5. A study on the dissolution rates of K-Cr(VI)-jarosites: kinetic analysis and implications.

    Science.gov (United States)

    Reyes, Iván A; Mireles, Ister; Patiño, Francisco; Pandiyan, Thangarasu; Flores, Mizraim U; Palacios, Elia G; Gutiérrez, Emmanuel J; Reyes, Martín

    2016-01-01

    The presence of natural and industrial jarosite type-compounds in the environment could have important implications in the mobility of potentially toxic elements such as lead, mercury, arsenic, chromium, among others. Understanding the dissolution reactions of jarosite-type compounds is notably important for an environmental assessment (for water and soil), since some of these elements could either return to the environment or work as temporary deposits of these species, thus would reduce their immediate environmental impact. This work reports the effects of temperature, pH, particle diameter and Cr(VI) content on the initial dissolution rates of K-Cr(VI)-jarosites (KFe3[(SO4)2 - X(CrO4)X](OH)6). Temperature (T) was the variable with the strongest effect, followed by pH in acid/alkaline medium (H3O(+)/OH(-)). It was found that the substitution of CrO4 (2-)in Y-site and the substitution of H3O(+) in M-site do not modify the dissolution rates. The model that describes the dissolution process is the unreacted core kinetic model, with the chemical reaction on the unreacted core surface. The dissolution in acid medium was congruent, while in alkaline media was incongruent. In both reaction media, there is a release of K(+), SO4 (2-) and CrO4 (2-) from the KFe3[(SO4)2 - X(CrO4)X](OH)6 structure, although the latter is rapidly absorbed by the solid residues of Fe(OH)3 in alkaline medium dissolutions. The dissolution of KFe3[(SO4)2 - X(CrO4)X](OH)6 exhibited good stability in a wide range of pH and T conditions corresponding to the calculated parameters of reaction order n, activation energy E A and dissolution rate constants for each kinetic stages of induction and progressive conversion. The kinetic analysis related to the reaction orders and calculated activation energies confirmed that extreme pH and T conditions are necessary to obtain considerably high dissolution rates. Extreme pH conditions (acidic or alkaline) cause the preferential release of K(+), SO4 (2

  6. Crystallization and preliminary X-ray analysis of Chandipura virus glycoprotein G.

    Science.gov (United States)

    Baquero, Eduard; Buonocore, Linda; Rose, John K; Bressanelli, Stéphane; Gaudin, Yves; Albertini, Aurélie A

    2012-09-01

    Fusion in members of the Rhabdoviridae virus family is mediated by the G glycoprotein. At low pH, the G glycoprotein catalyzes fusion between viral and endosomal membranes by undergoing a major conformational change from a pre-fusion trimer to a post-fusion trimer. The structure of the G glycoprotein from vesicular stomatitis virus (VSV G), the prototype of Vesiculovirus, has recently been solved in its trimeric pre-fusion and post-fusion conformations; however, little is known about the structural details of the transition. In this work, a soluble form of the ectodomain of Chandipura virus G glycoprotein (CHAV G(th)) was purified using limited proteolysis of purified virus; this soluble ectodomain was also crystallized. This protein shares 41% amino-acid identity with VSV G and thus its structure could provide further clues about the structural transition of rhabdoviral glycoproteins induced by low pH. Crystals of CHAV G(th) obtained at pH 7.5 diffracted X-rays to 3.1 Å resolution. These crystals belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 150.3, b = 228.2, c = 78.8 Å. Preliminary analysis of the data based on the space group and the self-rotation function indicated that there was no trimeric association of the protomers. This unusual oligomeric status could result from the presence of fusion intermediates in the crystal.

  7. Preliminary uncertainty analysis of OECD/UAM benchmark for the TMI-1 reactor

    Energy Technology Data Exchange (ETDEWEB)

    Cardoso, Fabiano S.; Faria, Rochkhudson B.; Silva, Lucas M.C.; Pereira, Claubia; Fortini, Angela, E-mail: claubia@nuclear.ufmg.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Departamento de Engenharia Nuclear

    2015-07-01

    Nowadays the demand from nuclear research centers for safety, regulation and better-estimated predictions provided with confidence bounds has been increasing. On that way, studies have pointed out that present uncertainties in the nuclear data should be significantly reduced, to get the full benefit from the advanced modeling and simulation initiatives. The major outcome of NEA/OECD (UAM) workshop took place Italy on 2006, was the preparation of a benchmark work program with steps (exercises) that would be needed to define the uncertainty and modeling tasks. On that direction, this work was performed within the framework of UAM Exercise 1 (I-1) 'Cell Physics' to validate the study, and to be able estimated the accuracies of the model. The objectives of this study were to make a preliminary analysis of criticality values of TMI-1 PWR and the biases of the results from two different nuclear codes multiplication factor. The range of the bias was obtained using the deterministic codes: NEWT (New ESC-based Weighting Transport code), the two-dimensional transport module that uses AMPX-formatted cross-sections processed by other SCALE; and WIMSD5 (Winfrith Improved Multi-Group Scheme) code. The WIMSD5 system consists of a simplified geometric representation of heterogeneous space zones that are coupled with each other and with the boundaries, while the properties of each spacing element are obtained from Carlson DSN method or Collision Probability method. (author)

  8. Global volcanic earthquake swarm database and preliminary analysis of volcanic earthquake swarm duration

    Directory of Open Access Journals (Sweden)

    S. R. McNutt

    1996-06-01

    Full Text Available Global data from 1979 to 1989 pertaining to volcanic earthquake swarms have been compiled into a custom-designed relational database. The database is composed of three sections: 1 a section containing general information on volcanoes, 2 a section containing earthquake swarm data (such as dates of swarm occurrence and durations, and 3 a section containing eruption information. The most abundant and reliable parameter, duration of volcanic earthquake swarms, was chosen for preliminary analysis. The distribution of all swarm durations was found to have a geometric mean of 5.5 days. Precursory swarms were then separated from those not associated with eruptions. The geometric mean precursory swarm duration was 8 days whereas the geometric mean duration of swarms not associated with eruptive activity was 3.5 days. Two groups of precursory swarms are apparent when duration is compared with the eruption repose time. Swarms with durations shorter than 4 months showed no clear relationship with the eruption repose time. However, the second group, lasting longer than 4 months, showed a significant positive correlation with the log10 of the eruption repose period. The two groups suggest that different suites of physical processes are involved in the generation of volcanic earthquake swarms.

  9. In situ fluidization for peat bed rupture, and preliminary economic analysis.

    Science.gov (United States)

    Niven, R K; Khalili, N

    2002-11-01

    This study concerns in situ fluidization (ISF), a new remediation method with potential application to the remediation of NAPL and heavy metal contaminants, by their release from the fluidized zone generated by a water jet. The present study examines the effect of ISF on layers of peat, of significance owing to its role as an important NAPL and metal contaminant trap. Once trapped, such contaminants are not readily accessible by most remedial methods, due to the low permeability and diffusivity of the peat. A simple tank experiment is used to demonstrate rupture of a peat layer by ISF, with removal of the peat as elutriated fines and segregated peat chunks. The application of ISF in the field is then examined by three field trials in uncontaminated sands, in both saturated and unsaturated conditions. Fluidized depths of up to 1.9 m in the saturated zone (with refusal on a peat layer) and 2.5 m in the unsaturated zone (no refusal) were attained, using a 1.9-m-long, 50 mm diameter jet operated at 5-13 1 s(-1). Pulses of dark turbidity and shell fragments in the effluent indicated the rupture of peat and shelly layers. The experiments demonstrate the hydraulic viability of ISF in the field, and its ability to remove peat-based contaminants. The issues of appropriate jet design and water generation during ISF are discussed, followed by a preliminary economic analysis of ISF relative to existing remediation methods.

  10. Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum

    Energy Technology Data Exchange (ETDEWEB)

    Pasquo, Alessandra [ENEA Casaccia Research Centre, Dipartimento BIOTEC, Sezione Genetica e Genomica Vegetale, PO Box 2400, I-00100 Rome (Italy); Bonamore, Alessandra; Franceschini, Stefano; Macone, Alberto; Boffi, Alberto; Ilari, Andrea, E-mail: andrea.ilari@uniroma1.it [Istituto di Biologia e Patologia Molecolari, CNR (IBPM) and Department Of Biochemical Sciences, University of Roma ‘La Sapienza’, Piazza Aldo Moro 5, 00179 Roma (Italy); ENEA Casaccia Research Centre, Dipartimento BIOTEC, Sezione Genetica e Genomica Vegetale, PO Box 2400, I-00100 Rome (Italy)

    2008-04-01

    The cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from T. flavum, a protein which catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, are reported. Norcoclaurine synthase (NCS) catalyzes the condensation of 3,4-dihydroxyphenylethylamine (dopamine) and 4-hydroxyphenylacetaldehyde (4-HPAA) as the first committed step in the biosynthesis of benzylisoquinoline alkaloids in plants. The protein was cloned, expressed and purified. Crystals were obtained at 294 K by the hanging-drop vapour-diffusion method using ammonium sulfate and sodium chloride as precipitant agents and diffract to better than 3.0 Å resolution using a synchrotron-radiation source. The crystals belong to the trigonal space group P3{sub 1}21, with unit-cell parameters a = b = 86.31, c = 118.36 Å. A selenomethionine derivative was overexpressed, purified and crystallized in the same space group. A complete MAD data set was collected at 2.7 Å resolution. The model is under construction.

  11. Acanthamoeba polyphaga mimivirus NDK: preliminary crystallographic analysis of the first viral nucleoside diphosphate kinase

    Energy Technology Data Exchange (ETDEWEB)

    Jeudy, Sandra [Information Génomique et Structurale, CNRS UPR 2589, 31 Chemin Joseph Aiguier, 13402 Marseille CEDEX 20 (France); Coutard, Bruno [Architecture et Fonction des Macromolecules Biologiques, CNRS UMR 6098, 31 Chemin Joseph Aiguier, 13402 Marseille CEDEX 20 (France); Lebrun, Régine [IBSM, 31 Chemin Joseph Aiguier, 13402 Marseille CEDEX 20 (France); Abergel, Chantal, E-mail: chantal.abergel@igs.cnrs-mrs.fr [Information Génomique et Structurale, CNRS UPR 2589, 31 Chemin Joseph Aiguier, 13402 Marseille CEDEX 20 (France)

    2005-06-01

    A. polyphaga mimivirus, the largest known double-stranded DNA virus, is the first virus to exhibit a nucleoside diphosphate kinase gene. The expression and crystallization of the viral NDK are reported. The complete sequence of the largest known double-stranded DNA virus, Acanthamoeba polyphaga mimivirus, has recently been determined [Raoult et al. (2004 ▶), Science, 306, 1344–1350] and revealed numerous genes not expected to be found in a virus. A comprehensive structural and functional study of these gene products was initiated [Abergel et al. (2005 ▶), Acta Cryst. F61, 212–215] both to better understand their role in the virus physiology and to obtain some clues to the origin of DNA viruses. Here, the preliminary crystallographic analysis of the viral nucleoside diphosphate kinase protein is reported. The crystal belongs to the cubic space group P2{sub 1}3, with unit-cell parameter 99.425 Å. The self-rotation function confirms that there are two monomers per asymmetric unit related by a twofold non-crystallographic axis and that the unit cell thus contains four biological entities.

  12. N-Acetyltransferase 2 status and gastric cancer risk: a preliminary meta-analysis

    Directory of Open Access Journals (Sweden)

    Stefania Boccia

    2005-03-01

    Full Text Available

    In recent studies N- Acetyltransferase 2 (NAT2 genotype has been considered as a risk factor for developing gastric cancer, however with conflicting results among Asian and Caucasian populations. In order to clarify the influence of NAT2 slow acetylation status on gastric cancer risk, a preliminary meta-analysis of published case-control studies was undertaken.

    The primary outcome measure was the odds ratio (OR for the risk of gastric cancer associated with the NAT2 slow genotype using a random effects model. Pooling the results from the 5 studies identified (771 cases, 1083 controls, an overall OR for gastric cancer risk associated with the NAT2 slow genotype of 0.91 emerged (95% CI: 0.54-1.55.

    The result suggests that the NAT2 slow genotype has probably no effect on the risk of gastric cancer. Additional epidemiological studies, based on sample sizes that are commensurate with the detection of small genotypic risks, are required to confirm these findings. Future studies may also help to clarify whether geographic differences exist.

  13. GC-MS Analysis and Preliminary Antimicrobial Activity of Albizia adianthifolia (Schumach and Pterocarpus angolensis (DC

    Directory of Open Access Journals (Sweden)

    Mustapha N. Abubakar

    2016-01-01

    Full Text Available The non-polar components of two leguminoceae species Albizia adianthifolia (Schumach, and Pterocarpus angolensis (DC were investigated. GC-MS analysis of the crude n-hexane and chloroform extracts together with several chromatographic separation techniques led to the identification and characterization (using NMR of sixteen known compounds from the heartwood and stem bark of Albizia adianthifolia and Pterocarpus angolensis respectively. These constituents include, n-hexadecanoic acid (palmitic acid 1, oleic acid 2, chondrillasterol 3, stigmasterol 4, 24S 5α-stigmast-7-en-3β-ol 5, 9,12-octadecadienoic acid (Z,Z-, methyl ester 6, trans-13-octadecanoic acid, methyl ester 7, tetradecanoic acid 8, hexadecanoic acid, methyl ester 9, octadecanoic acid 10, tetratriacontane 11, 7-dehydrodiosgenin 12, lupeol 13, stigmasta-3,5-diene-7-one 14, friedelan-3-one (friedelin 15, and 1-octacosanol 16. Using agar over lay method, the preliminary antimicrobial assay for the extracts was carried out against bacterial (E. coli, P. aeruginosa, B. subtilis, S. aueus and a fungus/yeast (C. albicans strains. The n-hexane and chloroform extracts of A. adianthifolia showed the best activity against E. coli with minimum inhibition quantity (MIQ of 1 µg each while the remaining exhibited moderate-to-weak activity against the test microorganisms.

  14. Satellite geological and geophysical remote sensing of Iceland: Preliminary results from analysis of MSS imagery

    Science.gov (United States)

    Williams, R. S., Jr.; Boedvarsson, A.; Fridriksson, S.; Palmason, G.; Rist, S.; Sigtryggsson, H.; Thorarinsson, S.; Thorsteinsson, I.

    1973-01-01

    A binational, multidisciplinary research effort in Iceland is directed at an analysis of MSS imagery from ERTS-1 to study a variety of geologic, hydrologic, oceanographic, and agricultural phenomena. A preliminary evaluation of available MSS imagery of Iceland has yielded several significant results - some of which may have direct importance to the Icelandic economy. Initial findings can be summarized as follows: (1) recent lava flows can be delineated from older flows at Askja and Hekla; (2) MSS imagery from ERTS-1 and VHRR visible and infrared imagery from NOAA-2 recorded the vocanic eruption on Heimaey, Vestmann Islands; (3) coastline changes, particularly changes in the position of bars and beaches along the south coast are mappable; and (4) areas covered with new and residual snow can be mapped, and the appearance of newly fallen snow on ERTS-1, MSS band 7 appears dark where it is melting. ERTS-1 imagery provides a means of updating various types of maps of Iceland and will permit the compilation of special maps specifically aimed at those dynamic environmental phenomena which impact on the Icelandic economy.

  15. Analysis of the preliminary optical links between ARTEMIS and the Optical Ground Station

    Science.gov (United States)

    Reyes Garcia-Talavera, Marcos; Chueca, Sergio; Alonso, Angel; Viera, Teodora; Sodnik, Zoran

    2002-12-01

    In the frame of the SILEX project, the European Space Agency (ESA) has put into orbit two Laser Communication Terminals, to establish an experimental free space optical communication link between a GEO satellite (ARTEMIS) and a LEO satellite (SPOT IV), to relay earth observation data. In order to perform In Orbit Testing (IOT) of these, and other, optical communications systems, ESA and the Instituto de Astrofisica de Canarias (IAC) reached an agreement for building the Optical Ground Station (OGS), in the Teide Observatory of the IAC. With ARTEMIS placed in a circular parking orbit at about 31000 kilometres, its optical payload has been preliminary tested with the OGS. First results and analysis are presented on the space-to-ground bi-directional link, including pointing acquisition and tracking performance, Bit-Error Rate (BER) and transmitted beam divergence effects related with atmospheric models and predictions. Future plans include deeper optical bi-directional communication tests of OGS, not only with ARTEMIS but also with OSCAR-40 (downlink) and SMART-1 (up-link) satellites, in order to do a full characterisation of the performances of laser beam propagation through atmospheric turbulence and a comparison with theoretical predictions.

  16. Marine Traffic Density Over Port Klang, Malaysia Using Statistical Analysis of AIS Data: A Preliminary Study

    Directory of Open Access Journals (Sweden)

    Masnawi MUSTAFFA

    2016-12-01

    Full Text Available Port Klang Malaysia is the 13th busiest port in the world, the capacity at the port expected to be able to meet the demand until 2018. It is one of the busiest ports in the world and also the busiest port in Malaysia. Even though there are statistics published by Port Klang Authority showing that a lot of ships using this port, this number is only based on ships that entering Port Klang. Therefore, no study has been done to investigate on how dense the traffic is in Port Klang, Malaysia the surrounding sea including Strait of Malacca . This paper has investigated on traffic density over Port Klang Malaysia and its surrounding sea using statistical analysis from AIS data. As a preliminary study, this study only collected AIS data for 7 days to represent daily traffic weekly. As a result, an hourly number of vessels, daily number of vessels, vessels classification and sizes and also traffic paths will be plotted.

  17. GC-MS Analysis and Preliminary Antimicrobial Activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC).

    Science.gov (United States)

    Abubakar, Mustapha N; Majinda, Runner R T

    2016-01-28

    The non-polar components of two leguminoceae species Albizia adianthifolia (Schumach), and Pterocarpus angolensis (DC) were investigated. GC-MS analysis of the crude n-hexane and chloroform extracts together with several chromatographic separation techniques led to the identification and characterization (using NMR) of sixteen known compounds from the heartwood and stem bark of Albizia adianthifolia and Pterocarpus angolensis respectively. These constituents include, n-hexadecanoic acid (palmitic acid) 1, oleic acid 2, chondrillasterol 3, stigmasterol 4, 24S 5α-stigmast-7-en-3β-ol 5, 9,12-octadecadienoic acid (Z,Z)-, methyl ester 6, trans-13-octadecanoic acid, methyl ester 7, tetradecanoic acid 8, hexadecanoic acid, methyl ester 9, octadecanoic acid 10, tetratriacontane 11, 7-dehydrodiosgenin 12, lupeol 13, stigmasta-3,5-diene-7-one 14, friedelan-3-one (friedelin) 15, and 1-octacosanol 16. Using agar over lay method, the preliminary antimicrobial assay for the extracts was carried out against bacterial (E. coli, P. aeruginosa, B. subtilis, S. aueus) and a fungus/yeast (C. albicans) strains. The n-hexane and chloroform extracts of A. adianthifolia showed the best activity against E. coli with minimum inhibition quantity (MIQ) of 1 µg each while the remaining exhibited moderate-to-weak activity against the test microorganisms.

  18. Bioinformatics analysis of the gene expression profile of hepatocellular carcinoma: preliminary results

    Science.gov (United States)

    Li, Jia

    2016-01-01

    Aim of the study To analyse the expression profile of hepatocellular carcinoma compared with normal liver by using bioinformatics methods. Material and methods In this study, we analysed the microarray expression data of HCC and adjacent normal liver samples from the Gene Expression Omnibus (GEO) database to screen for differentially expressed genes. Then, functional analyses were performed using GenCLiP analysis, Gene Ontology categories, and aberrant pathway identification. In addition, we used the CMap database to identify small molecules that can induce HCC. Results Overall, 2721 differentially expressed genes (DEGs) were identified. We found 180 metastasis-related genes and constructed co-occurrence networks. Several significant pathways, including the transforming growth factor β (TGF-β) signalling pathway, were identified as closely related to these DEGs. Some candidate small molecules (such as betahistine) were identified that might provide a basis for developing HCC treatments in the future. Conclusions Although we functionally analysed the differences in the gene expression profiles of HCC and normal liver tissues, our study is essentially preliminary, and it may be premature to apply our results to clinical trials. Further research and experimental testing are required in future studies. PMID:27095935

  19. Occupant evaluation of commercial office lighting: Volume 2, Preliminary data analysis

    Energy Technology Data Exchange (ETDEWEB)

    Marans, R.W.; Brown, M.A. (ed.)

    1987-11-01

    This report presents the preliminary results of a post-occupancy evaluation of office lighting environments. It explores the relationship between quantitative measures of lighting in occupied environments and qualitative measures related to occupant satisfaction. The report analyzes several types of data from more than 1000 occupied work stations: subjective data on attitudes and ratings of selected lighting and other characteristics, photometric and other direct environmental data, including illuminances, luminances, and contrast conditions, indirect environmental measures obtained from the architectural drawings and the work station photographs, and descriptive characteristics of the occupants. The work stations were sampled from thirteen office buildings located in various cities in the United States. Many tentative findings emerged from the analysis, including the following: (1) within the range of values examined here, there is a tendency for lighting satisfaction to decrease as lighting power density increases; (2) occupants who described their work station spaces as bright also tended to be satisfied with their work station lighting; (3) occupants who were most bothered by bright lights and glare were most likely to express dissatisfaction with the lighting at their work stations; (4) there is no relationship between work-related activities of employees and indicators of lighting quality. More research is needed before firm conclusions can be drawn and before guidance regarding lighting standards and other policy issues can be derived. 3 refs., 9 figs.

  20. Hydrothermal Liquefaction and Upgrading of Municipal Wastewater Treatment Plant Sludge: A Preliminary Techno-Economic Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Snowden-Swan, Lesley J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Zhu, Yunhua [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Jones, Susanne B. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Elliott, Douglas C. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Schmidt, Andrew J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hallen, Richard T. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Billing, Justin M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hart, Todd R. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Fox, Samuel P. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Maupin, Gary D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-06-08

    A preliminary process model and techno-economic analysis (TEA) was completed for fuel produced from hydrothermal liquefaction (HTL) of sludge waste from a municipal wastewater treatment plant (WWTP) and subsequent biocrude upgrading. The model is adapted from previous work by Jones et al. (2014) for algae HTL, using experimental data generated in fiscal year 2015 (FY15) bench-scale HTL testing of sludge waste streams. Testing was performed on sludge samples received from MetroVancouver’s Annacis Island WWTP (Vancouver, B.C.) as part of a collaborative project with the Water Environment and Reuse Foundation (WERF). The full set of sludge HTL testing data from this effort will be documented in a separate report to be issued by WERF. This analysis is based on limited testing data and therefore should be considered preliminary. Future refinements are necessary to improve the robustness of the model, including a cross-check of modeled biocrude components with the experimental GCMS data and investigation of equipment costs most appropriate at the smaller scales used here. Environmental sustainability metrics analysis is also needed to understand the broader impact of this technology pathway. The base case scenario for the analysis consists of 10 HTL plants, each processing 100 dry U.S. ton/day (92.4 ton/day on a dry, ash-free basis) of sludge waste and producing 234 barrel per stream day (BPSD) biocrude, feeding into a centralized biocrude upgrading facility that produces 2,020 barrel per standard day of final fuel. This scale was chosen based upon initial wastewater treatment plant data collected by the resource assessment team from the EPA’s Clean Watersheds Needs Survey database (EPA 2015a) and a rough estimate of what the potential sludge availability might be within a 100-mile radius. In addition, we received valuable feedback from the wastewater treatment industry as part of the WERF collaboration that helped form the basis for the selected HTL and upgrading

  1. Thermochemistry and Kinetic Analysis of the Unimolecular Oxiranyl Radical Dissociation Reaction: A Theoretical Study.

    Science.gov (United States)

    Wang, Heng; Bozzelli, Joseph W

    2016-07-04

    Oxirane structures are important in organic synthesis, and they are important initial products in the oxidation reactions of alkyl radicals. The thermochemical properties (enthalpy of formation, entropy, and heat capacity) for the reaction steps of the unimolecular oxiranyl radical dissociation reaction are determined and compared with the available literature. The overall ring opening and subsequent steps involve four types of reactions: β-scission ring opening, intramolecular hydrogen transfer, β-scission hydrogen elimination, and β-scission methyl radical elimination. The enthalpies of formation of the transition states are determined and evaluated using six popular Density Functional Theory (DFT) calculation methods (B3LYP, B2PLYP, M06, M06-2X, ωB97X, ωB97XD), each combined with three different basis sets. The DFT enthalpy values are compared with five composite calculation methods (G3, G4, CBS-QB3, CBS-APNO, W1U), and by CCSD(T)/aug-cc-pVTZ. Kinetic parameters are determined versus pressure and temperature for the unimolecular dissociation pathways of an oxiranyl radical, which include the chemical activation reactions of the ring-opened oxiranyl radical relative to the ring-opening barrier. Multifrequency quantum Rice Ramsperger Kassel (QRRK) analysis is used to determine k(E) with master equation analysis for falloff. The major overall reaction pathway at lower combustion temperatures is oxiranyl radical dissociation to a methyl radical and carbon monoxide. Oxiranyl radical dissociation to a ketene and hydrogen atom is the key reaction path above 700 K.

  2. Brook trout (Salvelinus fontinalis extinction in small boreal lakes revealed by ephippia pigmentation: a preliminary analysis

    Directory of Open Access Journals (Sweden)

    Alexandre Bérubé Tellier

    2016-12-01

    Full Text Available Ephippium pigmentation is a plastic trait which can be related to a trade-off between visual predation pressure and better protection of cladoceran eggs against different types of stress. Experimental studies showed that planktivorous fish exert a greater predation pressure on individuals carrying darker ephippia, but little is known about the variation of ephippium pigmentation along gradients of fish predation pressure in natural conditions. For this study, our experimental design included four small boreal lakes with known fish assemblages. Two of the lakes have viable brook trout (Salvelinus fontinalis populations, whereas the other two lakes experienced brook trout extinctions during the 20th century. Cladoceran ephippia were extracted from sediment cores at layers corresponding to the documented post- extinction phase (1990's and from an older layer (1950's for which the brook trout population status is not known precisely. Our first objective was to determine whether brook trout extinction has a direct effect on both ephippium pigmentation and size. Our second objective was to give a preliminary assessment of the status of brook trout populations in the 1950's by comparing the variation in ephippia traits measured from this layer to those measured in the 1990's, for which the extinction patterns are well known. Cost-effective image analysis was used to assess variation in pigmentation levels in ephippia. This approach provided a proxy for the amount of melanin invested in each ephippium analysed. Our study clearly shows that ephippium pigmentation may represent a better indicator of the presence of fish predators than ephippium size, a trait that showed a less clear pattern of variation between lakes with and without fish. For the 1990's period, ephippia from fishless lakes were darker and showed a slight tendency to be larger than ephippia from lakes with brook trout. However, no clear differences in either ephippium size or pigmentation

  3. Fractal kinetic analysis of the enzymatic saccharification of CO2 laser pretreated corn stover.

    Science.gov (United States)

    Tian, Shuang-Qi; Ma, Sen; Wang, Xin-Wei; Zhang, Zheng-Nan

    2013-10-15

    The enzymatic hydrolyses of laser pretreated corn stover as a novel pretreatment method were examined to establish a simplified kinetic model for the complicated hydrolysis process. The time dependence of the total reducing sugars amount was closely related to the amounts of cellulosic materials and amounts of cellulase. The evaluated model fitted very well with the experimental data of enzymatic hydrolysis of laser pretreated corn stover under different conditions, including cellulase loading, nature of substrate, substrate loading in the reaction medium. The results indicated that the complex kinetics of cellulase enzymatic saccharification could be assessed with the fractal kinetic model. The cellulase enzymatic reaction process was effectively predicted and controlled with the kinetic model. The result showed that the model could effectively reflect dynamic process of enzyme hydrolysis.

  4. Thermogravimetric analysis and kinetic study of formation of lithium titanate by solid state route

    Energy Technology Data Exchange (ETDEWEB)

    Sonak, Sagar; Jain, Uttam [Fusion Reactor Materials Section, Bhabha Atomic Research Centre, Mumbai 400 085 (India); Sahu, Ashok Kumar [Glass and advanced Materials Division, Bhabha Atomic Research Centre, Mumbai 400 085 (India); Kumar, Sanjay; Krishnamurthy, Nagaiyar [Fusion Reactor Materials Section, Bhabha Atomic Research Centre, Mumbai 400 085 (India)

    2015-02-15

    The kinetics of formation of lithium titanate from the solid state reaction of lithium carbonate and titanium oxide was studied using non-isothermal thermogravimetric technique. Thermogravimetric data for the reaction of lithium carbonate and titanium oxide was obtained at various heating rates. The methods such as Flynn–Wall–Ozawa and Kissinger–Akahira–Sunose were used to estimate the kinetic parameters from the obtained thermogravimetric data. The average activation energy for the formation of lithium titanate by solid state route was found to be 243 kJ/mol K. The reaction mechanism was determined by the method given by Malek. It was found that the three dimensional diffusion model best describes the reaction kinetics. A kinetic equation describing the reaction is proposed and reaction mechanism is discussed.

  5. Analysis of Stability for Gas-Kinetic Non-Local Traffic Model

    Institute of Scientific and Technical Information of China (English)

    SUN Xi-Ming; DONG Yu-Jie

    2006-01-01

    @@ The gas-kinetic non-local traffic model is improved by taking into account the relative velocity of the correlated vehicles. The stability of different relaxation time modes is analytically investigated with the perturbation method.

  6. Energetic and exergetic performance analysis and modeling of drying kinetics of kiwi slices

    National Research Council Canada - National Science Library

    Darvishi, Hosain; Zarein, Mohammad; Farhudi, Zanyar

    2016-01-01

    This work focused on the effects of the moisture content, slices thickness and microwave power on aspects of energy and exergy, drying kinetics, moisture diffusivity, activation energy, and modeling...

  7. Dissolution kinetics and thermodynamic analysis of vanadium trioxide during pressure oxidation

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    The dissolution kinetics of vanadium trioxide in sulphuric acid-oxygen medium was examined. It was determined that the concentration of sulphuric acid and stirring speed above 800 r min 1 did not significantly affect vanadium extraction. The dissolution rate increased with increasing temperature and oxygen partial pressure, but decreased with increasing particle size. The dissolution kinetics was controlled by the chemical reaction at the surface with the estimated activation energy of 43.46 kJ·mol-1. The l...

  8. Preliminary Analysis of Effects of Reduced Discharge onThermal Habitat of Pedersen Warm Springs Channel

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A preliminary report to study the potential impacts of possible flow reductions in thermal spring systems located in the Warm Springs area of Moapa Valley NWR on the...

  9. Pharmacognostic and Preliminary Phytochemical Analysis of Sauropus androgynus (L) Merr. Leaf

    OpenAIRE

    Ankad Gireesh; Hegde Harsha; Kholkute S.D; Hurkadale Pramod

    2013-01-01

    The leaves of Sauropus androgynus (L.) Merr. are used in traditional medicine to treat various disorders and also used as vegetable for its nutritive value. Such plant having medicinal and nutritive values lacks pharmacognostical and preliminary phytochemical information. Hence the present work is intended to study pharmacognostical and preliminary phytochemical studies, which will serve as quality control parameters. The pharmacognostical parameters like transverse section of midrib, epiderm...

  10. Error structure of enzyme kinetic experiments. Implications for weighting in regression analysis of experimental data.

    Science.gov (United States)

    Askelöf, P; Korsfeldt, M; Mannervik, B

    1976-10-01

    assumption of proportionality between variance and valpha (where alpha is an empirically determined exponent) was a good approximation for the weighting. The value of alpha was 1.6 in the present case. The weight function was tested in the fitting of a rate equation to a kinetic-data set involving variable substrate concentrations. Recommendations are given regarding the establishment of the error structure in a general case and its application in regression analysis.

  11. Volume analysis of heat-induced cracks in human molars: A preliminary study

    Directory of Open Access Journals (Sweden)

    Michael A. Sandholzer

    2014-01-01

    Full Text Available Context: Only a few methods have been published dealing with the visualization of heat-induced cracks inside bones and teeth. Aims : As a novel approach this study used nondestructive X-ray microtomography (micro-CT for volume analysis of heat-induced cracks to observe the reaction of human molars to various levels of thermal stress. Materials and Methods: Eighteen clinically extracted third molars were rehydrated and burned under controlled temperatures (400, 650, and 800°C using an electric furnace adjusted with a 25°C increase/min. The subsequent high-resolution scans (voxel-size 17.7 μm were made with a compact micro-CT scanner (SkyScan 1174. In total, 14 scans were automatically segmented with Definiens XD Developer 1.2 and three-dimensional (3D models were computed with Visage Imaging Amira 5.2.2. The results of the automated segmentation were analyzed with an analysis of variance (ANOVA and uncorrected post hoc least significant difference (LSD tests using Statistical Package for Social Sciences (SPSS 17. A probability level of P < 0.05 was used as an index of statistical significance. Results: A temperature-dependent increase of heat-induced cracks was observed between the three temperature groups (P < 0.05, ANOVA post hoc LSD. In addition, the distributions and shape of the heat-induced changes could be classified using the computed 3D models. Conclusion: The macroscopic heat-induced changes observed in this preliminary study correspond with previous observations of unrestored human teeth, yet the current observations also take into account the entire microscopic 3D expansions of heat-induced cracks within the dental hard tissues. Using the same experimental conditions proposed in the literature, this study confirms previous results, adds new observations, and offers new perspectives in the investigation of forensic evidence.

  12. Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study.

    Science.gov (United States)

    Zelig, Udi; Barlev, Eyal; Bar, Omri; Gross, Itai; Flomen, Felix; Mordechai, Shaul; Kapelushnik, Joseph; Nathan, Ilana; Kashtan, Hanoch; Wasserberg, Nir; Madhala-Givon, Osnat

    2015-05-15

    Most of the blood tests aiming for breast cancer screening rely on quantification of a single or few biomarkers. The aim of this study was to evaluate the feasibility of detecting breast cancer by analyzing the total biochemical composition of plasma as well as peripheral blood mononuclear cells (PBMCs) using infrared spectroscopy. Blood was collected from 29 patients with confirmed breast cancer and 30 controls with benign or no breast tumors, undergoing screening for breast cancer. PBMCs and plasma were isolated and dried on a zinc selenide slide and measured under a Fourier transform infrared (FTIR) microscope to obtain their infrared absorption spectra. Differences in the spectra of PBMCs and plasma between the groups were analyzed as well as the specific influence of the relevant pathological characteristics of the cancer patients. Several bands in the FTIR spectra of both blood components significantly distinguished patients with and without cancer. Employing feature extraction with quadratic discriminant analysis, a sensitivity of ~90 % and a specificity of ~80 % for breast cancer detection was achieved. These results were confirmed by Monte Carlo cross-validation. Further analysis of the cancer group revealed an influence of several clinical parameters, such as the involvement of lymph nodes, on the infrared spectra, with each blood component affected by different parameters. The present preliminary study suggests that FTIR spectroscopy of PBMCs and plasma is a potentially feasible and efficient tool for the early detection of breast neoplasms. An important application of our study is the distinction between benign lesions (considered as part of the non-cancer group) and malignant tumors thus reducing false positive results at screening. Furthermore, the correlation of specific spectral changes with clinical parameters of cancer patients indicates for possible contribution to diagnosis and prognosis.

  13. Preliminary design of a small air loop for system analysis and validation of Cathare code

    Energy Technology Data Exchange (ETDEWEB)

    Marchand, M.; Saez, M.; Tauveron, N.; Tenchine, D.; Germain, T.; Geffraye, G.; Ruby, G.P. [CEA Grenoble (DEN/DER/SSTH), 38 (France)

    2007-07-01

    The French Atomic Energy Commission (Cea) is carrying on the design of a Small Air Loop for System Analysis (SALSA), devoted to the study of gas cooled nuclear reactors behaviour in normal and incidental/accidental operating conditions. The reduced size of the SALSA components compared to a full-scale reactor and air as gaseous coolant instead of Helium will allow an easy management of the loop. The main purpose of SALSA will be the validation of the associated thermal hydraulic safety simulation codes, like CATHARE. The main goal of this paper is to present the methodology used to define the characteristics of the loop. In a first step, the study has been focused on a direct-cycle system for the SALSA loop with few global constraints using a similarity analysis to support the definition and design of the loop. Similarity requirements have been evaluated to determine the scale factors which have to be applied to the SALSA loop components. The preliminary conceptual design of the SALSA plant with a definition of each component has then be carried out. The whole plant has been modelled using the CATHARE code. Calculations of the SALSA steady-state in nominal conditions and of different plant transients in direct-cycle have been made. The first system results obtained on the global behaviour of the loop confirm that SALSA can be representative of a Gas-Cooled nuclear reactor with some minor design modifications. In a second step, the current prospects focus on the SALSA loop capability to reproduce correctly the heat transfer occurring in specific incidental situations. Heat decay removal by natural convection is a crucial point of interest. The first results show that the behaviour and the efficiency of the loop are strongly influenced by the definition of the main parameters for each component. A complete definition of SALSA is under progress. (authors)

  14. An evaluation of the Positive Emotional Experiences Scale: A preliminary analysis

    Directory of Open Access Journals (Sweden)

    Rene van Wyk

    2016-04-01

    Full Text Available Orientation: The positive organisational behaviour movement emphasises the advantages of psychological strengths in business. The psychological virtues of positive emotional experiences can potentially promote human strengths to the advantages of business functioning and the management of work conditions. This is supported by Fredrickson’s broaden-and-build theory that emphasises the broadening of reactive thought patterns through experiences of positive emotions.Research purpose: A preliminary psychometric evaluation of a positive measurement of dimensions of emotional experiences in the workplace, by rephrasing the Kiefer and Barclay Toxic Emotional Experiences Scale.Motivation for the study: This quantitative Exploratory Factor Analysis investigates the factorial structure and reliability of the Positive Emotional Experiences Scale, a positive rephrased version of the Toxic Emotional Experiences Scale.Research approach, design and method: This Exploratory Factor Analysis indicates an acceptable three-factor model for the Positive Emotional Experiences Scale. These three factors are: (1 psychological recurrent positive state, (2 social connectedness and (3 physical refreshed energy, with strong Cronbach’s alphas of 0.91, 0.91 and 0.94, respectively.Main findings: The three-factor model of the Positive Emotional Experiences Scale provides a valid measure in support of Fredrickson’s theory of social, physical and psychological endured personal resources that build positive emotions.Practical/Managerial implications: Knowledge gained on positive versus negative emotional experiences could be applied by management to promote endured personal resources that strengthen positive emotional experiences.Contribution/value-add: The contribution of this rephrased Positive Emotional Experiences Scale provides a reliable measure of assessment of the social, physical and endured psychological and personal resources identified in Fredrickson’s broaden

  15. Crystallization and preliminary X-ray diffraction analysis of L-threonine dehydrogenase (TDH) from the hyperthermophilic archaeon Thermococcus kodakaraensis.

    Science.gov (United States)

    Bowyer, A; Mikolajek, H; Wright, J N; Coker, A; Erskine, P T; Cooper, J B; Bashir, Q; Rashid, N; Jamil, F; Akhtar, M

    2008-09-01

    The enzyme L-threonine dehydrogenase catalyses the NAD(+)-dependent conversion of L-threonine to 2-amino-3-ketobutyrate, which is the first reaction of a two-step biochemical pathway involved in the metabolism of threonine to glycine. Here, the crystallization and preliminary crystallographic analysis of L-threonine dehydrogenase (Tk-TDH) from the hyperthermophilic organism Thermococcus kodakaraensis KOD1 is reported. This threonine dehydrogenase consists of 350 amino acids, with a molecular weight of 38 kDa, and was prepared using an Escherichia coli expression system. The purified native protein was crystallized using the hanging-drop vapour-diffusion method and crystals grew in the tetragonal space group P4(3)2(1)2, with unit-cell parameters a = b = 124.5, c = 271.1 A. Diffraction data were collected to 2.6 A resolution and preliminary analysis indicates that there are four molecules in the asymmetric unit of the crystal.

  16. SENSITIVITY ANALYSIS OF KINETIC CONSTANTS AS A TOOL FOR ELUCIDATING THE POLYMERIZATION MECHANISM OF ACRYL-FURANIC COMPOUNDS

    Directory of Open Access Journals (Sweden)

    Jurgen Lange

    2013-12-01

    Full Text Available By means of the sensitivity analysis of kinetics constants in a proposed mechanism for radical polymerization of acrylfuranic compounds [Furfuryl Acrylate (FA and Methacrylate (FM],it is elucidated which elementary steps are relevant in the phenomenology. In this analysis, the application of Come's methodology allows to classify the elementary steps of a mechanism in three categories: Non-sensible, Non-determinant, Sensible. The results obtained with this tool in modeling of experimental data in free radical polymerization of FA and FM suggest that kinetic mechanism consists mainly on five elementary steps: 1 Primary initiation, 2 propagation, 3 degradative transfers (which include intermolecular and primary, 4 re-initiation and 5 cross-termination. Thus, taking into account these elementary steps in mathematical modeling, the polymerization of FA and FM in different experimental conditions was successfully simulated.

  17. Kinetic performance appraisal of poly(styrene-co-divinylbenzene) monolithic high-performance liquid chromatography columns for biomolecule analysis.

    Science.gov (United States)

    Causon, Tim J; Shellie, Robert A; Hilder, Emily F

    2010-06-11

    A broad appraisal of the kinetic performance of organic polymeric monolithic columns is reported using commercially available poly(styrene-co-divinylbenzene) monolithic columns (Dionex ProSwift RP-1S). Analysis of a protein digest sample at elevated temperatures (> or =80 degrees C) indicated no apparent analyte degradation using an inert polymeric stationary phase. Comparison between low molecular weight solute and peptide separations highlighted the markedly different mass transport processes observed on macroporous monolithic beds and an improved C term at elevated temperature in both instances. The current usefulness of this column format for biomolecule analysis was further studied via employment of a kinetic performance characterisation for the first time to provide direction for column development servicing this application.

  18. Kinetic-Thermodynamic Analysis of the Reactive Distillation Process of the Cyclohexene Hydration Using the Zeolite Catalyst

    Institute of Scientific and Technical Information of China (English)

    叶建初; 黄佳丽; 林晗丹; 曹克腾; 沙勇

    2011-01-01

    Reactive distillation could be utilized to produce cyclohexanol through the cyclohexene hydration. By means of highly active zeolite catalyst HZSM-5, the kinetic-thermodynamic analysis of this reactive distillation has been carried out to get the characteristics of the reactive distillation. Results from kinetic and thermodynamic analysis indicate that the optimal pressure of this reactive distillation process should be set to higher pressure such as 0.3 or 0.4 MPa. To avoid the recovery of cyclohexanol at the top of the column, an unreactive section should be allocated at the upper column. In addition, the inert component benzene is more unfavorable to the reactive distillation process in comparison with the inert cyclohexane.

  19. - and Syn-Eruptive Surface Movements of Azerbaijan Mud Volcanoes Detected Through Insar Analysis: Preliminary Results

    Science.gov (United States)

    Antonielli, Benedetta; Monserrat, Oriol; Bonini, Marco; Righini, Gaia; Sani, Federico; Luzi, Guido; Feyzullayev, Akper; Aliyev, Chingiz

    2014-05-01

    Mud volcanism is a process that consists in the extrusion of mud, fragments or blocks of country rocks, saline waters and gases, mostly methane. This mechanism is typically linked to in-depth hydrocarbon traps, and it builds up a variety of conical edifices with dimension and morphology similar to those of magmatic volcanoes. Interferometry by Satellite Aperture Radar (InSAR) techniques have been commonly used to monitor and investigate the ground deformation connected to the eruptive phases of magmatic volcanoes. InSAR techniques have also been employed to explore the ground deformation associated with the LUSI mud volcano in Java (Indonesia). We aim to carry out a study on the paroxysmal activities of the Azerbaijan mud volcanoes, among the largest on Earth, using similar techniques. In particular the deformations of the mud volcanic systems were analyzed through the technique of satellite differential interferometry (DInSAR), thanks to the acquisition of 16 descending and 4 ascending Envisat images, spanning about 4 years (October 2003-November 2007); these data were provided by the European Space Agency. The preliminary analysis of a set of 77 interferograms and the unwrapping process elaboration of some of them selected according to the best coherence values, allowed the detection of significant deformations in correspondence of Ayaz-Akhtarma and Khara Zira Island mud volcanoes. This analysis has allowed to identify relevant ground deformations of the volcanic systems in connection with the main eruptive events in 2005 and in 2006 respectively, that are recorded by the catalogue of Azerbaijan mud volcano eruptions until 2007. The preliminary analysis of the interferograms of the Ayaz-Akhtarma and the Khara Zira mud volcanoes shows that the whole volcano edifice or part of it is subject to a ground displacement before or in coincidence with the eruption. Assuming that the movement is mainly vertical, we suppose that deformation is due to bulging of the volcanic

  20. Flow cytometric analysis of oil palm: a preliminary analysis for cultivars and genomic DNA alteration

    Directory of Open Access Journals (Sweden)

    Warawut Chuthammathat

    2005-12-01

    Full Text Available DNA contents of oil palm (Elaeis guineensis Jacq. cultivars were analyzed by flow cytometry using different external reference plant species. Analysis using corn (Zea mays line CE-777 as a reference plant gave the highest DNA content of oil palm (4.72±0.23 pg 2C-1 whereas the DNA content was found to be lower when using soybean (Glycine max cv. Polanka (3.77±0.09 pg 2C-1 or tomato (Lycopersicon esculentum cv. Stupicke (4.25±0.09 pg 2C-1 as a reference. The nuclear DNA contents of Dura (D109, Pisifera (P168 and Tenera (T38 cultivars were 3.46±0.04, 3.24±0.03 and 3.76±0.04 pg 2C-1 nuclei, respectively, using soybean as a reference. One haploid genome of oil palm therefore ranged from 1.56 to 1.81±109 base pairs. DNA contents from one-year-old calli and cell suspension of oil palm were found to be significantly different from those of seedlings. It thus should be noted that genomic DNA alteration occurred in these cultured tissues. We therefore confirm that flow cytometric analysis could verify cultivars, DNA content and genomic DNA alteration of oil palm using soybean as an external reference standard.

  1. Applications of Photogrammetry for Analysis of Forest Plantations. Preliminary study: Analysis of individual trees

    Science.gov (United States)

    Mora, R.; Barahona, A.; Aguilar, H.

    2015-04-01

    This paper presents a method for using high detail volumetric information, captured with a land based photogrammetric survey, to obtain information from individual trees. Applying LIDAR analysis techniques it is possible to measure diameter at breast height, height at first branch (commercial height), basal area and volume of an individual tree. Given this information it is possible to calculate how much of that tree can be exploited as wood. The main objective is to develop a methodology for successfully surveying one individual tree, capturing every side of the stem a using high resolution digital camera and reference marks with GPS coordinates. The process is executed for several individuals of two species present in the metropolitan area in San Jose, Costa Rica, Delonix regia (Bojer) Raf. and Tabebuia rosea (Bertol.) DC., each one with different height, stem shape and crown area. Using a photogrammetry suite all the pictures are aligned, geo-referenced and a dense point cloud is generated with enough detail to perform the required measurements, as well as a solid tridimensional model for volume measurement. This research will open the way to develop a capture methodology with an airborne camera using close range UAVs. An airborne platform will make possible to capture every individual in a forest plantation, furthermore if the analysis techniques applied in this research are automated it will be possible to calculate with high precision the exploit potential of a forest plantation and improve its management.

  2. Preliminary gait analysis results after posterior tibial tendon reconstruction: a prospective study.

    Science.gov (United States)

    Brodsky, James W

    2004-02-01

    The purpose of this study was to investigate the effect on gait in patients who underwent reconstruction for stage II posterior tibial tendon (PTT) dysfunction. Twelve patients with stage II PTT dysfunction underwent surgical reconstruction consisting of debridement of the posterior tibial tendon, flexor digitorum longus tendon transfer to the navicular tuberosity, medial displacement calcaneal osteotomy, and spring ligament reconstruction. Midfoot arthrodesis was performed in six patients and gastrocnemius recession in three. Gait analysis was performed 2 weeks prior to surgery and 1 year postoperatively. Preoperative and postoperative data were compared to determine differences in temporal-spatial parameters, lower limb kinematics, and ankle push-off power. Step length for the operated side increased from 52.6 +/- 9.6 cm before the surgery to 57.5 +/- 7.1 cm after the surgery (p =.048). Cadence improved from 100.2 +/- 10.7 steps/min to 109.1 +/- 8.5 steps/min (p =.05), thus increasing velocity from 87.6 +/- 22.6 cm/s to 103.4 +/- 15.9 cm/s (p =.042). Single support percentage was unchanged. Maximum sagittal ankle joint power at push-off increased from 0.79 +/- 0.35 W before surgery to 1.2 +/- 0.5 W after surgery (p =.042). There were statistically significant improvements in all radiographic parameters studied. This is the first prospective study to evaluate the in vivo effects on gait in patients undergoing this common surgical procedure. Analysis demonstrated statistically significant improvement in kinetic and kinematic parameters of gait function.

  3. Kinetic analysis and molecular modeling of the inhibition mechanism of roneparstat (SST0001) on human heparanase

    Science.gov (United States)

    Pala, Daniele; Rivara, Silvia; Mor, Marco; Milazzo, Ferdinando Maria; Roscilli, Giuseppe; Pavoni, Emiliano; Giannini, Giuseppe

    2016-01-01

    Heparanase is a β-d-glucuronidase which cleaves heparan sulfate chains in the extracellular matrix and on cellular membranes. A dysregulated heparanase activity is intimately associated with cell invasion, tumor metastasis and angiogenesis, making heparanase an attractive target for the development of anticancer therapies. SST0001 (roneparstat; Sigma-Tau Research Switzerland S.A.) is a non-anticoagulant 100% N-acetylated and glycol-split heparin acting as a potent heparanase inhibitor, currently in phase I in advanced multiple myeloma. Herein, the kinetics of heparanase inhibition by roneparstat is reported. The analysis of dose-inhibition curves confirmed the high potency of roneparstat (IC50 ≈ 3 nM) and showed, at higher concentrations, a Hill coefficient consistent with the engagement of two molecules of inhibitor. A homology model of human heparanase GS3 construct was built and used for docking experiments with inhibitor fragments. The model has high structural similarity with the recently reported crystal structure of human heparanase. Different interaction schemes are proposed, which support the hypothesis of a complex binding mechanism involving the recruitment of one or multiple roneparstat chains, depending on its concentration. In particular, docking solutions were obtained in which (i) a single roneparstat molecule interacts with both heparin-binding domains (HBDs) of heparanase or (ii) two fragments of roneparstat interact with either HBD-1 or HBD-2, consistent with the possibility of different inhibitor:enzyme binding stoichiometries. This study provides unique insights into the mode of action of roneparstat as well as clues of its interaction with heparanase at a molecular level, which could be exploited to design novel potential inhibitor molecules. PMID:26762172

  4. Devolatilization Characteristics and Kinetic Analysis of Lump Coal from China COREX3000 Under High Temperature

    Science.gov (United States)

    Xu, Runsheng; Zhang, Jianliang; Wang, Guangwei; Zuo, Haibin; Liu, Zhengjian; Jiao, Kexin; Liu, Yanxiang; Li, Kejiang

    2016-08-01

    A devolatilization study of two lump coals used in China COREX3000 was carried out in a self-developed thermo-gravimetry at four temperature conditions [1173 K, 1273 K, 1373 K, and 1473 K (900 °C, 1000 °C, 1100 °C, and 1200 °C)] under N2. This study reveals that the working temperature has a strong impact on the devolatilization rate of the lump coal: the reaction rate increases with the increasing temperature. However, the temperature has little influence on the maximum mass loss. The conversion rate curve shows that the reaction rate of HY lump coal is higher than KG lump coal. The lump coals were analyzed by XRD, FTIR, and optical microscopy to explore the correlation between devolatilization rate and properties of lump coal. The results show that the higher reaction rate of HY lump coal attributes to its more active maceral components, less aromaticity and orientation degree of the crystallite, and more oxygenated functional groups. The random nucleation and nuclei growth model (RNGM), volume model (VM), and unreacted shrinking core model (URCM) were employed to describe the reaction behavior of lump coal. It was concluded from kinetics analysis that RNGM model was the best model for describing the devolatilization of lump coals. The apparent activation energies of isothermal devolatilization of HY lump coal and KG lump coal are 42.35 and 45.83 kJ/mol, respectively. This study has implications for the characteristics and mechanism modeling of devolatilization of lump coal in COREX gasifier.

  5. Stepwise binding of tylosin and erythromycin to Escherichia coli ribosomes, characterized by kinetic and footprinting analysis.

    Science.gov (United States)

    Petropoulos, Alexandros D; Kouvela, Ekaterini C; Dinos, George P; Kalpaxis, Dimitrios L

    2008-02-22

    Erythromycin and tylosin are 14- and 16-membered lactone ring macrolides, respectively. The current work shows by means of kinetic and chemical footprinting analysis that both antibiotics bind to Escherichia coli ribosomes in a two-step process. The first step established rapidly, involves a low-affinity binding site placed at the entrance of the exit tunnel in the large ribosomal subunit, where macrolides bind primarily through their hydrophobic portions. Subsequently, slow conformational changes mediated by the antibiotic hydrophilic portion push the drugs deeper into the tunnel, in a high-affinity site. Compared with erythromycin, tylosin shifts to the high-affinity site more rapidly, due to the interaction of the mycinose sugar of the drug with the loop of H35 in domain II of 23 S rRNA. Consistently, mutations of nucleosides U2609 and U754 implicated in the high-affinity site reduce the shift of tylosin to this site and destabilize, respectively, the final drug-ribosome complex. The weak interaction between tylosin and the ribosome is Mg2+ independent, unlike the tight binding. In contrast, both interactions between erythromycin and the ribosome are reduced by increasing concentrations of Mg2+ ions. Polyamines attenuate erythromycin affinity for the ribosome at both sequential steps of binding. In contrast, polyamines facilitate the initial binding of tylosin, but exert a detrimental, more pronounced, effect on the drug accommodation at its final position. Our results emphasize the role of the particular interactions that side chains of tylosin and erythromycin establish with 23 S rRNA, which govern the exact binding process of each drug and its response to the ionic environment.

  6. Electron kinetics dependence on gas pressure in laser-induced oxygen plasma experiment: Theoretical analysis

    Science.gov (United States)

    Gamal, Yosr E. E.-D.; Abdellatif, Galila

    2017-08-01

    A study is performed to investigate the dependency of threshold intensity on gas pressure observed in the measurements of the breakdown of molecular oxygen that carried out by Phuoc (2000) [1]. In this experiment, the breakdown was induced by 532 nm laser radiation of pulse width 5.5 ns and spot size of 8.5 μm, in oxygen over a wide pressure range (190-3000 Torr). The analysis aimed to explore the electron kinetic reliance on gas pressure for the separate contribution of each of the gain and loss processes encountered in this study. The investigation is based on an electron cascade model applied previously in Gamal and Omar (2001) [2] and Gaabour et al. (2013) [3]. This model solves numerically a differential equation designates the time evolution of the electron energy distribution, and a set of rate equations that describe the change of excited states population. The numerical examination of the electron energy distribution function and its parameters revealed that photo-ionization of the excited molecules plays a significant role in enhancing the electron density growth rate over the whole tested gas pressure range. This process is off set by diffusion of electrons out of the focal volume in the low-pressure regime. At atmospheric pressure electron, collisional processes dominate and act mainly to populate the excited states. Hence photo-ionization becomes efficient and compete with the encountered loss processes (electron diffusion, vibrational excitation of the ground state molecules as well as two body attachments). At high pressures ( 3000 Torr) three body attachments are found to be the primary cause of losses which deplete the electron density and hence results in the slow decrease of the threshold intensity.

  7. The Importance of Growth Kinetic Analysis in Determining Bacterial Susceptibility against Antibiotics and Silver Nanoparticles

    Directory of Open Access Journals (Sweden)

    Karsten eTheophel

    2014-11-01

    Full Text Available Routine antibiotics susceptibility testing still relies on standardized cultivation-based analyses, including measurement of inhibition zones in conventional agar diffusion tests and endpoint turbidity-based measurements. Here, we demonstrate that common off-line monitoring and endpoint determination after 18–24 h could be insufficient for reliable growth-dependent evaluation of antibiotic susceptibility. Different minimal inhibitory concentrations were obtained in 20- and 48-h microdilution plate tests using an Enterococcus faecium clinical isolate (strain UKI-MB07 as a model organism. Hence, we used an on-line kinetic assay for simultaneous cultivation and time-resolved growth analysis in a 96-well format instead of off-line susceptibility testing. Growth of the Enterococcus test organism was delayed up to 30 h in the presence of 0.25 µg mL-1 of vancomycin and 8 µg mL-1 of fosfomycin, after which pronounced growth was observed. Despite the delayed onset of growth, treatment with fosfomycin, daptomycin, fusidic acid, cefoxitin, or gentamicin resulted in higher maximum growth rates and/or higher final optical density values compared with antibiotic-free controls, indicating that growth stimulation and hormetic effects may occur with extended exposure to sublethal antibiotic concentrations. Whereas neither maximum growth rate nor final cell density correlated with antibiotic concentration, the lag phase duration for some antibiotics was a more meaningful indicator of dose-dependent growth inhibition. Our results also reveal that non-temporal growth profiles are only of limited value for cultivation-based antimicrobial silver nanoparticle susceptibility testing. The exposure to Ag(0 nanoparticles led to plasma membrane damage in a concentration-dependent manner and induced oxidative stress in Enterococcus faecium UKI-MB07, as shown by intracellular ROS accumulation.

  8. High-throughput quantitative analysis with cell growth kinetic curves for low copy number mutant cells.

    Science.gov (United States)

    Xing, James Z; Gabos, Stephan; Huang, Biao; Pan, Tianhong; Huang, Min; Chen, Jie

    2012-10-01

    The mutation rate in cells induced by environmental genotoxic hazards is very low and difficult to detect using traditional cell counting assays. The established genetic toxicity tests currently recognized by regulatory authorities, such as conventional Ames and hypoxanthine guanine phosphoribosyl-transferase (HPRT) assays, are not well suited for higher-throughput screening as they require large amounts of test compounds and are very time consuming. In this study, we developed a novel cell-based assay for quantitative analysis of low numbers of cell copies with HPRT mutation induced by an environmental mutagen. The HPRT gene mutant cells induced by the mutagen were selected by 6-thioguanine (6-TG) and the cell's kinetic growth curve monitored by a real-time cell electronic sensor (RT-CES) system. When a threshold is set at a certain cell index (CI) level, samples with different initial mutant cell copies take different amounts of time in order for their growth (or CI accumulation) to cross this threshold. The more cells that are initially seeded in the test well, the faster the cell accumulation and therefore the shorter the time required to cross this threshold. Therefore, the culture time period required to cross the threshold of each sample corresponds to the original number of cells in the sample. A mutant cell growth time threshold (MT) value of each sample can be calculated to predict the number of original mutant cells. For mutagenesis determination, the RT-CES assay displayed an equal sensitivity (p > 0.05) and coefficients of variation values with good correlation to conventional HPRT mutagenic assays. Most importantly, the RT-CES mutation assay has a higher throughput than conventional cellular assays.

  9. Preliminary clinical application of contrast-enhanced MR angiography using 3D time-resolved imaging of contrast kinetics(3D-TRICKS)

    Institute of Scientific and Technical Information of China (English)

    YANG Chun-shan; LIU Shi-yuan; XIAO Xiang-sheng; FENG Yun; LI Hui-min; XIAO Shan; GONG Wan-qing

    2007-01-01

    Objective: To introduce a new better contrast-enhanced MR angiographic method, named 3D time-resolved imaging of contrast kinetics (3D-TRICKS). Methods: TRICKS is a high temporal resolution (2-6 s) MR angiographic technique using a short TR(4 ms) and TE(1.5 ms), partial echo sampling, in which central part of k-space is updated more frequently than the peripheral part. TRICKS pre-contrast mask 3D images are firstly scanned, and then the bolus injecting of Gd-DTPA, 15-20 sequential 3D images are acquired. The reconstructed 3D images, subtraction of contrast 3D images with mask images, are conceptually similar to a catheter-based intra-arterial digital subtraction angiographic series(DSA). Thirty patients underwent contrast-enhanced MR angiography using 3D-TRICKS. Results: Totally 12 vertebral arteries were well displayed on TRICKS, in which 7 were normal, 1 demonstrated bilateral vertebral artery stenosis, 4 had unilateral vertebral artery stenosis and 1 was accompanied with the same lateral carotid artery bifurcation stenosis. Four cases of bilateral renal arteries were normal, 1 transplanted kidney artery showed as normal and 1 transplanted kidney artery showed stenosis. 2 cerebral arteries were normal, 1 had sagittal sinus thrombosis and 1 displayed intracranial arteriovenous malformation. 3 pulmonary arteries were normal, 1 showed pulmonary artery thrombosis and 1 revealed pulmonary sequestration's abnormal feeding artery and draining vein. One left lower limb fibrolipoma showed feeding artery. One displayed radial-ulnar artery artificial fistula stenosis. One revealed left antebrachium hemangioma. Conclusion: TRICKS can clearly delineate most body vascular system and reveal most vascular abnormality. It possesses convenience and high successful rate, which make it the first choice of displaying most vascular abnormality.

  10. Preliminary risk analysis applied to the handling of health-care waste

    Directory of Open Access Journals (Sweden)

    Carvalho S.M.L.

    2002-01-01

    Full Text Available Between 75% and 90% of the waste produced by health-care providers no risk or is "general" health-care waste, comparable to domestic waste. The remaining 10-25% of health-care waste is regarded as hazardous due to one or more of the following characteristics: it may contain infectious agents, sharps, toxic or hazardous chemicals or it may be radioactive. Infectious health-care waste, particularly sharps, has been responsible for most of the accidents reported in the literature. In this work the preliminary risks analysis (PRA technique was used to evaluate practices in the handling of infectious health-care waste. Currently the PRA technique is being used to identify and to evaluate the potential for hazard of the activities, products, and services from facilities and industries. The system studied was a health-care establishment which has handling practices for infectious waste. Thirty-six procedures related to segregation, containment, internal collection, and storage operation were analyzed. The severity of the consequences of the failure (risk that can occur from careless management of infectious health-care waste was classified into four categories: negligible, marginal, critical, and catastrophic. The results obtained in this study showed that events with critics consequences, about 80%, may occur during the implementation of the containment operation, suggesting the need to prioritize this operation. As a result of the methodology applied in this work, a flowchart the risk series was also obtained. In the flowchart the events that can occur as a consequence of a improper handling of infectious health-care waste, which can cause critical risks such as injuries from sharps and contamination (infection from pathogenic microorganisms, are shown.

  11. Stochastic index model for intermittent regimes: from preliminary analysis to regionalisation

    Directory of Open Access Journals (Sweden)

    M. Rianna

    2011-04-01

    Full Text Available In small and medium-sized basins or in rivers characterized by intermittent discharges, with low or negligible/null observed values for long periods of the year, the correct representation of the discharge regime is important for issues related to water management and to define the amount and quality of water available for irrigation, domestic and recreational uses. In these cases, only one index as a statistical metric is often not enough; it is thus necessary to introduce Flow Duration Curves (FDC.

    The aim of this study is therefore to combine a stochastic index flow model capable of reproducing the FDC record period of a river, regardless of the persistence and seasonality of the series, with the theory of total probability in order to calculate how often a river is dry.

    The paper draws from preliminary analyses, including a study to estimate the correlation between discharge indicators Q95, Q50 and Q1 (discharges exceeding 95%, 50% or 1% of the time, respectively and some fundamental characteristics of the basin, as well as to identify homogeneous regions in the target area through the study of several geo-morphological features and climatic conditions. The stochastic model was then applied in one of the homogeneous regions that includes intermittent rivers.

    Finally, the model was regionalized by means of regression analysis in order to calculate the FDC for ungauged basins; the reliability of this method was tested using jack-knife validation.

  12. Identification of succinic semialdehyde reductases from Geobacter: expression,purification, crystallization, preliminary functional, and crystallographic analysis

    Institute of Scientific and Technical Information of China (English)

    Yanfeng Zhang; Xiaoli Gao; Yi Zheng; R. Michae; Garavito

    2011-01-01

    Succinic semialdehyde reductase (SSAR) is an important enzyme involved in γ-aminobutyrate (GABA) metabolism.By converting succinic semialdehyde (SSA) to γ-hydroxybutyrate (GHB),the SSAR facilitates an alternative pathway for GABA degradation.In this study,we identified SSARs from Geobacter sulfurreducens and Geobacter metallireducens (GsSSAR and GmSSAR,respectively).The enzymes were over-expressed in Escherichia coil and purified to near homogeneity.Both GsSSAR and GmSSAR showed the activity of reducing SSA using nicotinamide adenine dinucleotide phosphate as a co-factor.The oligomeric sizes of GsSSAR and GmSSAR,as determined by analytical size exclusion chromatography,suggest that the enzymes presumably exist as tetramers in solution.The recombinant GsSSAR and GmSSAR crystallized in the presence of NADP+,and the resulting crystals diffracted to 1.89 (A) (GsSSAR) and 2.25 (A)(GmSSAR) resolution.The GsSSAR and GmSSAR crystals belong to the space groups P21221 (a =99.61 (A),b =147.49 (A),c =182.47 A) and P1 (a =75.97 (A) b =79.14 (A) c =95.47 (A),α =82.15°,β =88.80°,γ=87.66°),respectively.Preliminary crystallographic data analysis suggests the presence of eight protein monomers in the asymmetric units for both GsSSAR and GmSSAR.

  13. Identification of succinic semialdehyde reductases from Geobacter: expression, purification, crystallization, preliminary functional, and crystallographic analysis

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yanfeng; Gao, Xiaoli; Zheng, Yi; Garavito, R. Michael (MSU)

    2012-04-30

    Succinic semialdehyde reductase (SSAR) is an important enzyme involved in {gamma}-aminobutyrate (GABA) metabolism. By converting succinic semialdehyde (SSA) to {gamma}-hydroxybutyrate (GHB), the SSAR facilitates an alternative pathway for GABA degradation. In this study, we identified SSARs from Geobacter sulfurreducens and Geobacter metallireducens (GsSSAR and GmSSAR, respectively). The enzymes were over-expressed in Escherichia coli and purified to near homogeneity. Both GsSSAR and GmSSAR showed the activity of reducing SSA using nicotinamide adenine dinucleotide phosphate as a co-factor. The oligomeric sizes of GsSSAR and GmSSAR, as determined by analytical size exclusion chromatography, suggest that the enzymes presumably exist as tetramers in solution. The recombinant GsSSAR and GmSSAR crystallized in the presence of NADP{sup +}, and the resulting crystals diffracted to 1.89 {angstrom} (GsSSAR) and 2.25 {angstrom} (GmSSAR) resolution. The GsSSAR and GmSSAR crystals belong to the space groups P2{sub 1}22{sub 1} (a = 99.61 {angstrom}, b = 147.49 {angstrom}, c = 182.47 {angstrom}) and P1 (a = 75.97 {angstrom}, b = 79.14 {angstrom}, c = 95.47 {angstrom}, {alpha} = 82.15{sup o}, {beta} = 88.80{sup o}, {gamma} = 87.66{sup o}), respectively. Preliminary crystallographic data analysis suggests the presence of eight protein monomers in the asymmetric units for both GsSSAR and GmSSAR.

  14. Preliminary Analysis of the Transient Reactor Test Facility (TREAT) with PROTEUS

    Energy Technology Data Exchange (ETDEWEB)

    Connaway, H. M. [Argonne National Lab. (ANL), Argonne, IL (United States); Lee, C. H. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2015-11-30

    The neutron transport code PROTEUS has been used to perform preliminary simulations of the Transient Reactor Test Facility (TREAT). TREAT is an experimental reactor designed for the testing of nuclear fuels and other materials under transient conditions. It operated from 1959 to 1994, when it was placed on non-operational standby. The restart of TREAT to support the U.S. Department of Energy’s resumption of transient testing is currently underway. Both single assembly and assembly-homogenized full core models have been evaluated. Simulations were performed using a historic set of WIMS-ANL-generated cross-sections as well as a new set of Serpent-generated cross-sections. To support this work, further analyses were also performed using additional codes in order to investigate particular aspects of TREAT modeling. DIF3D and the Monte-Carlo codes MCNP and Serpent were utilized in these studies. MCNP and Serpent were used to evaluate the effect of geometry homogenization on the simulation results and to support code-to-code comparisons. New meshes for the PROTEUS simulations were created using the CUBIT toolkit, with additional meshes generated via conversion of selected DIF3D models to support code-to-code verifications. All current analyses have focused on code-to-code verifications, with additional verification and validation studies planned. The analysis of TREAT with PROTEUS-SN is an ongoing project. This report documents the studies that have been performed thus far, and highlights key challenges to address in future work.

  15. Purification, crystallization and preliminary structural analysis of nucleoside diphosphate kinase from Bacillus anthracis

    Energy Technology Data Exchange (ETDEWEB)

    Misra, Gauri [Molecular and Structural Biology Division, Central Drug Research Institute, PO Box 173, Chattar Manzil, Mahatma Gandhi Marg, Lucknow 226 001 (India); Aggarwal, Anita [Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007 (India); Mittal, Sonia [Molecular and Structural Biology Division, Central Drug Research Institute, PO Box 173, Chattar Manzil, Mahatma Gandhi Marg, Lucknow 226 001 (India); Singh, Yogendra [Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007 (India); Ramachandran, Ravishankar, E-mail: r-ravishankar@cdri.res.in [Molecular and Structural Biology Division, Central Drug Research Institute, PO Box 173, Chattar Manzil, Mahatma Gandhi Marg, Lucknow 226 001 (India)

    2007-12-01

    Nucleoside diphosphate kinase from B. anthracis has been crystallized. Preliminary crystallographic analysis shows that there is one monomer in the asymmetric unit of the crystal. Bacillus anthracis nucleoside diphosphate kinase (BaNdk) is an enzyme whose primary function is to maintain deoxynucleotide triphosphate (dNTP) pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor. Although the structures of Ndks from a variety of organisms have been elucidated, the enzyme from sporulating bacteria has not been structurally characterized to date. Crystals of the B. anthracis enzyme were grown using the vapour-diffusion method from a hanging drop consisting of 2 µl 10 mg ml{sup −1} protein in 50 mM Tris–HCl pH 8.0, 50 mM NaCl, 5 mM EDTA equilibrated against 500 µl reservoir solution consisting of 2.25 M ammonium formate and 0.1 M HEPES buffer pH 7.25. Diffraction data extending to 2.0 Å were collected at room temperature from a single crystal with unit-cell parameters a = b = 107.53, c = 52.3 Å. The crystals are hexagonal in shape and belong to space group P6{sub 3}22. The crystals contain a monomer in the asymmetric unit, which corresponds to a Matthews coefficient (V{sub M}) of 2.1 Å{sup 3} Da{sup −1} and a solvent content of about 36.9%.

  16. A new scoring system in Cystic Fibrosis: statistical tools for database analysis - a preliminary report.

    Science.gov (United States)

    Hafen, G M; Hurst, C; Yearwood, J; Smith, J; Dzalilov, Z; Robinson, P J

    2008-10-05

    Cystic fibrosis is the most common fatal genetic disorder in the Caucasian population. Scoring systems for assessment of Cystic fibrosis disease severity have been used for almost 50 years, without being adapted to the milder phenotype of the disease in the 21st century. The aim of this current project is to develop a new scoring system using a database and employing various statistical tools. This study protocol reports the development of the statistical tools in order to create such a scoring system. The evaluation is based on the Cystic Fibrosis database from the cohort at the Royal Children's Hospital in Melbourne. Initially, unsupervised clustering of the all data records was performed using a range of clustering algorithms. In particular incremental clustering algorithms were used. The clusters obtained were characterised using rules from decision trees and the results examined by clinicians. In order to obtain a clearer definition of classes expert opinion of each individual's clinical severity was sought. After data preparation including expert-opinion of an individual's clinical severity on a 3 point-scale (mild, moderate and severe disease), two multivariate techniques were used throughout the analysis to establish a method that would have a better success in feature selection and model derivation: 'Canonical Analysis of Principal Coordinates' and 'Linear Discriminant Analysis'. A 3-step procedure was performed with (1) selection of features, (2) extracting 5 severity classes out of a 3 severity class as defined per expert-opinion and (3) establishment of calibration datasets. (1) Feature selection: CAP has a more effective "modelling" focus than DA.(2) Extraction of 5 severity classes: after variables were identified as important in discriminating contiguous CF severity groups on the 3-point scale as mild/moderate and moderate/severe, Discriminant Function (DF) was used to determine the new groups mild, intermediate moderate, moderate, intermediate

  17. A new scoring system in Cystic Fibrosis: statistical tools for database analysis – a preliminary report

    Science.gov (United States)

    Hafen, GM; Hurst, C; Yearwood, J; Smith, J; Dzalilov, Z; Robinson, PJ

    2008-01-01

    Background Cystic fibrosis is the most common fatal genetic disorder in the Caucasian population. Scoring systems for assessment of Cystic fibrosis disease severity have been used for almost 50 years, without being adapted to the milder phenotype of the disease in the 21st century. The aim of this current project is to develop a new scoring system using a database and employing various statistical tools. This study protocol reports the development of the statistical tools in order to create such a scoring system. Methods The evaluation is based on the Cystic Fibrosis database from the cohort at the Royal Children's Hospital in Melbourne. Initially, unsupervised clustering of the all data records was performed using a range of clustering algorithms. In particular incremental clustering algorithms were used. The clusters obtained were characterised using rules from decision trees and the results examined by clinicians. In order to obtain a clearer definition of classes expert opinion of each individual's clinical severity was sought. After data preparation including expert-opinion of an individual's clinical severity on a 3 point-scale (mild, moderate and severe disease), two multivariate techniques were used throughout the analysis to establish a method that would have a better success in feature selection and model derivation: 'Canonical Analysis of Principal Coordinates' and 'Linear Discriminant Analysis'. A 3-step procedure was performed with (1) selection of features, (2) extracting 5 severity classes out of a 3 severity class as defined per expert-opinion and (3) establishment of calibration datasets. Results (1) Feature selection: CAP has a more effective "modelling" focus than DA. (2) Extraction of 5 severity classes: after variables were identified as important in discriminating contiguous CF severity groups on the 3-point scale as mild/moderate and moderate/severe, Discriminant Function (DF) was used to determine the new groups mild, intermediate moderate

  18. A new scoring system in Cystic Fibrosis: statistical tools for database analysis – a preliminary report

    Directory of Open Access Journals (Sweden)

    Yearwood J

    2008-10-01

    Full Text Available Abstract Background Cystic fibrosis is the most common fatal genetic disorder in the Caucasian population. Scoring systems for assessment of Cystic fibrosis disease severity have been used for almost 50 years, without being adapted to the milder phenotype of the disease in the 21st century. The aim of this current project is to develop a new scoring system using a database and employing various statistical tools. This study protocol reports the development of the statistical tools in order to create such a scoring system. Methods The evaluation is based on the Cystic Fibrosis database from the cohort at the Royal Children's Hospital in Melbourne. Initially, unsupervised clustering of the all data records was performed using a range of clustering algorithms. In particular incremental clustering algorithms were used. The clusters obtained were characterised using rules from decision trees and the results examined by clinicians. In order to obtain a clearer definition of classes expert opinion of each individual's clinical severity was sought. After data preparation including expert-opinion of an individual's clinical severity on a 3 point-scale (mild, moderate and severe disease, two multivariate techniques were used throughout the analysis to establish a method that would have a better success in feature selection and model derivation: 'Canonical Analysis of Principal Coordinates' and 'Linear Discriminant Analysis'. A 3-step procedure was performed with (1 selection of features, (2 extracting 5 severity classes out of a 3 severity class as defined per expert-opinion and (3 establishment of calibration datasets. Results (1 Feature selection: CAP has a more effective "modelling" focus than DA. (2 Extraction of 5 severity classes: after variables were identified as important in discriminating contiguous CF severity groups on the 3-point scale as mild/moderate and moderate/severe, Discriminant Function (DF was used to determine the new groups mild

  19. Preliminary Evaluation of MapReduce for High-Performance Climate Data Analysis

    Science.gov (United States)

    Duffy, D. Q.; Schnase, J. L.; Clune, T. L.; Kim, E. J.; Freeman, S. M.; Thompson, J. H.; Hunter, K. A.; Theriot, M. E.

    2011-12-01

    Data intensive analytic workflows bridge between the largely unstructured mass of stored scientific data and the highly structured, tailored, reduced, and refined products used by scientists in their research. In general, the initial steps of an analysis, those operations that first interact with a data repository, tend to be the most general, while data manipulations closer to the client tend to be the most specialized to the individual, to the domain, or to the science question under study. The amount of data being operated on also tends to be larger on the repository-side of the workflow, smaller toward the client-side end products. We are using MapReduce to exploit this natural stratification, optimize efficiencies along the workflow chain, and provide a preliminary qualitative and quantitative assessment of MapReduce as a means of enabling server-side, distributed climate data analysis. MapReduce is a model for distributed storage and computation that seeks to improve efficiencies of the near-archive operations that initiate workflows. Simply put, MapReduce stores chunked data on disks with associated processors in such a way that operations on the chunked data can occur in parallel and return meaningfully aggregated results. While MapReduce has proven effective for large repositories of textual data, its use in data intensive science applications has been limited, because many scientific data sets are inherently complex, have high dimensionality, and use binary formats. We are using Apache's open-source Hadoop software implementation of MapReduce on top of the Hadoop Filesystem in our evaluation. Our analyses focus on soil moisture, precipitation, and atmospheric water-vapor, important classes of observation- and simulation-derived data products. The specific data sets being used in the evaluation include MERRA monthly precipitation and soil moisture products; the MODIS Atmospheres, 8-day global water-vapor product; and the SMOS 3-day global soil moisture

  20. Sensitivity Analysis for Reactor Period Induced by Positive Reactivity Using One-point Adjoint Kinetic Equation

    Science.gov (United States)

    Chiba, G.; Tsuji, M.; Narabayashi, T.

    2014-04-01

    In order to better predict a kinetic behavior of a nuclear fission reactor, an improvement of the delayed neutron parameters is essential. The present paper specifies important nuclear data for a reactor kinetics: Fission yield and decay constant data of 86Ge, some bromine isotopes, 94Rb, 98mY and some iodine isotopes. Their importance is quantified as sensitivities with a help of the adjoint kinetic equation, and it is found that they are dependent on an inserted reactivity (or a reactor period). Moreover, dependence of sensitivities on nuclear data files is also quantified using the latest files. Even though the currently evaluated data are used, there are large differences among different data files from a view point of the delayed neutrons.

  1. A kinetic energy analysis of the meso beta-scale severe storm environment

    Science.gov (United States)

    Fuelberg, H. E.; Printy, M. F.

    1984-01-01

    Analyses are performed of the meso beta-scale (20-200 km wavelengths and several hours to one-day periods) severe storm kinetic energy balance on the fifth day of the AVE SESAME campaign of May 1979. A 24-hr interval covering the antecedent, active and post-convective outbreak activity over Oklahoma are considered. Use is made of the kinetic energy budget equation (KEBE) for a finite volume in an isobaric coordinate system. Rawindsonde data with 75 km resolution were treated. The KEBE model covered changes in kinetic energy due to the cross contour flows, horizontal and vertical components of flux divergence, and volumic mass changes on synoptic and subsynoptic scales. The greatest variability was concentrated above 400 mb height and over the most intense storm activity. Energy was generated at the highest rates in divergence and decreased the most in convection. The meso beta-scale lacked sufficient resolution for analyzing mesoscale activity.

  2. Kinetic analysis of an anion exchange absorbent for CO2 capture from ambient air.

    Science.gov (United States)

    Shi, Xiaoyang; Li, Qibin; Wang, Tao; Lackner, Klaus S

    2017-01-01

    This study reports a preparation method of a new moisture swing sorbent for CO2 capture from air. The new sorbent components include ion exchange resin (IER) and polyvinyl chloride (PVC) as a binder. The IER can absorb CO2 when surrounding is dry and release CO2 when surrounding is wet. The manuscript presents the studies of membrane structure, kinetic model of absorption process, performance of desorption process and the diffusivity of water molecules in the CO2 absorbent. It has been proved that the kinetic performance of CO2 absorption/desorption can be improved by using thin binder and hot water treatment. The fast kinetics of P-100-90C absorbent is due to the thin PVC binder, and high diffusion rate of H2O molecules in the sample. The impressive is this new CO2 absorbent has the fastest CO2 absorption rate among all absorbents which have been reported by other up-to-date literatures.

  3. SINGLE-SHELL TANK INTEGRITY PROJECT ANALYSIS OF RECORD-PRELIMINARY MODELING PLAN FOR THERMAL AND OPERATING LOADS

    Energy Technology Data Exchange (ETDEWEB)

    RAST RS; RINKER MW; BAPANAALLI SK; DEIBLER JE; GUZMAN-LEONG CE; JOHNSON KI; KARRI NK; PILLI SP; SANBORN SE

    2010-10-22

    This document is a Phase I deliverable for the Single-Shell Tank Analysis of Record effort. This document is not the Analysis of Record. The intent of this document is to guide the Phase II detailed modeling effort. Preliminary finite element models for each of the tank types were developed and different case studies were performed on one or more of these tank types. Case studies evaluated include thermal loading, waste level variation, the sensitivity of boundary effects (soil radial extent), excavation slope or run to rise ratio, soil stratigraphic (property and layer thickness) variation at different farm locations, and concrete material property variation and their degradation under thermal loads. The preliminary analysis document reviews and preliminary modeling analysis results are reported herein. In addition, this report provides recommendations for the next phase of the SST AOR project, SST detailed modeling. Efforts and results discussed in this report do not include seismic modeling as seismic modeling is covered by a separate report. The combined results of both static and seismic models are required to complete this effort. The SST AOR project supports the US Department of Energy's (DOE) Office of River Protection (ORP) mission for obtaining a better understanding of the structural integrity of Hanford's SSTs. The 149 SSTs, with six different geometries, have experienced a range of operating histories which would require a large number of unique analyses to fully characterize their individual structural integrity. Preliminary modeling evaluations were conducted to determine the number of analyses required for adequate bounding of each of the SST tank types in the Detailed Modeling Phase of the SST AOR Project. The preliminary modeling was conducted in conjunction with the Evaluation Criteria report, Johnson et al. (2010). Reviews of existing documents were conducted at the initial stage of preliminary modeling. These reviews guided the topics

  4. How do Organizational Learning and Market Conditions Affect the Relationship between Entrepreneurial Orientation and Firm Growth? A Preliminary Analysis on Small and Medium Size Hotels in Peninsular Malaysia

    National Research Council Canada - National Science Library

    Azilah Kasim; Levent Altinay

    2016-01-01

      This is a preliminary analysis on the influence of organizational learning and market conditions on the relationships between entrepreneurial orientation and growth of small and medium size hotels...

  5. Kinetic analysis of the reactions of hypobromous acid with protein components

    DEFF Research Database (Denmark)

    Pattison, David I; Davies, Michael Jonathan

    2004-01-01

    in proteins isolated from patients with atherosclerosis, asthma, and cystic fibrosis, implicating the production of HOX in these diseases. The quantitative significance of these findings requires knowledge of the kinetics of reaction of HOX with protein targets, and such data have not been previously...... are more, and Cys and Met much less, important targets for HOBr than HOCl. Kinetic models have been developed to predict the targets of HOX attack on proteins and free amino acids. Overall, these results shed light on the mechanisms of cell damage induced by HOX and indicate, for example, that the 3-chloro...

  6. A phenomenological analysis of sintering kinetics from the viewpoint of activated volume

    Directory of Open Access Journals (Sweden)

    Nikolić M.V.

    2005-01-01

    Full Text Available The sintering kinetics of real systems has been viewed as a process of transport of activated volume. Activated volume is a parameter that can be used to describe mass transport during the sintering process. It defines the movement of point defects and dislocations during the sintering process. A phenomenological equation has been defined using this parameter, which can be applied to analyze kinetics of the sintering process. It has been applied to analyze the sintering process of several disperse systems. Values obtained for parameters of the equation have also been analyzed.

  7. Analysis of Self Similar Scaling in Kinetic and Magnetic Energy Density as a Function of Distance From Sun

    Science.gov (United States)

    Banerjee, A.; Coplan, M. A.

    2009-12-01

    We analyze solar wind and interplanetary magnetic field data to study scaling properties of kinetic and magnetic energy density as a function of solar cycle and distance from the sun. In his original theory on turbulence, Kolmogorov predicted that in the inertial range the fluctuations in velocity differences should be self-similar. Analysis of solar wind data showed this not to be the case. On the other hand B. Hnat et.al.(Geophys. Res. Lett., 29 (10), 1446, 2002) and J.J Podesta (J. Geophys. Res., 111, A09105, 2006) showed that fluctuations in kinetic and magnetic energy density are approximately self-similar. We extend this analysis using data from the SWE and MFI experiments on the WIND spacecraft (at 1AU) during solar minimum (2006) and solar maximum (2001) and VHM/FGM experiment on the Ulysses spacecraft (1AU to 5AU). We calculate the cumulative distribution function (CDF) of the time delayed differences in kinetic and magnetic energy density and present a method through which the scaling exponent can be reliably calculated from the CDFs, instead of using structure functions which are very sensitive to large fluctuations. We compare the scaling exponents derived from the CDFs to the ones calculated from structure functions and study the rescaling properties of CDFs.

  8. A Critical View on In Vitro Analysis of P-glycoprotein (P-gp) Transport Kinetics.

    Science.gov (United States)

    Saaby, Lasse; Brodin, Birger

    2017-09-01

    Transport proteins expressed in the different barriers of the human body can have great implications on absorption, distribution, and excretion of drug compounds. Inhibition or saturation of a transporter can potentially alter these absorbtion, distribution, metabolism and elimination properties and thereby also the pharmacokinetic profile and bioavailability of drug compounds. P-glycoprotein (P-gp, ABCB1) is an efflux transporter which is present in most of the barriers of the body, including the small intestine, the blood-brain barrier, the liver, and the kidney. In all these tissues, P-gp may mediate efflux of drug compounds and may also be a potential site for drug-drug interactions. Consequently, there is a need to be able to predict the saturation and inhibition of P-gp and other transporters in vivo. For this purpose, Michaelis-Menten steady-state analysis has been applied to estimate kinetic parameters, such as Km and Vmax, for carrier-mediated transport, whereas half-maximal inhibitor concentration (IC50) and the disassociation constant for an inhibitor/P-gp complex (Ki) have been determined to estimate P-gp inhibition. This review addresses in vitro methods commonly used to study P-gp transport kinetics and aims at providing a critical evaluation of the application of steady-state Michaelis-Menten analysis of kinetic parameters for substrate/P-gp interactions. Copyright © 2017 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.

  9. Analysis of rainfall-induced shallow landslides in Jamne and Jaszcze stream valleys (Polish Carpathians – preliminary results

    Directory of Open Access Journals (Sweden)

    Zydroń Tymoteusz

    2016-03-01

    Full Text Available Analysis of rainfall-induced shallow landslides in Jamne and Jaszcze stream valleys (Polish Carpathians - preliminary results. Preliminary shallow landslide susceptibility mapping of the Jamne and Jaszcze stream valleys, located in the Polish Flysch Carpathians, is presented in the paper. For the purpose of mapping, there were used SINMAP and Iverson’s models integrating infiltration and slope stability calculations. The calibration of the used models parameters, obtained from limited field and laboratory tests, was performed using data from 8-9 July 1997, when as a consequence of a very intense rainfall, 94 shallow landslides were observed on meadows and arable lands. A comparison of the slope stability calculation results and the localisation of the noticed shallow landslides showed satisfactory agreement between localisation of the observed and computed unstable areas. However, it was concluded that better simulation results were obtained using Iverson’s model.

  10. Analysis of the operation of a diffraction-radiation generator with preliminary HF modulation

    Science.gov (United States)

    Pivovarova, A. G.

    1991-05-01

    The excitation conditions and output characteristics of a diffraction-radiation generator with preliminary HF modulation are determined. Attention is given to the effects of the modulation parameter, the phase shift, and the aiming distance on the displacement of the generation zone, variations of the minimum starting current, the frequency shift, the oscillation amplitude, and the efficiency of the generator. It is shown that the efficiency of a generator with preliminary HF modulation can be twice as high as that of a generator without modulation.

  11. Pathway kinetics and metabolic control analysis of a high-yielding strain of Penicillium chrysogenum during fed-batch cultivations

    DEFF Research Database (Denmark)

    Pissarra, Pedro de N.; Nielsen, Jens Bredal; Bazin, M. J.

    1996-01-01

    on the rate of the reaction catalyzed by this enzyme, and consequently as an enhancer of the specific rate of penicillin production. Using the kinetic model, metabolic control analysis (MCA) of the pathway was performed. The determined flux control coefficients suggested that, during the production phase......A kinetic model representing the pathway for the biosynthesis of penicillin by P. chrysogenum has been developed. The model is capable of describing the flux through the biosynthetic pathway, and model simulations correspond well with measurements of intermediates and end products. One feature......, the flux is controlled by IPNS as this enzyme becomes saturated with tripeptide delta-(L-alpha-amino-adipyl)-L-cysteinyl-D-valine (LLD-ACV). In the simulations, oxygen was shown to be a bottleneck alleviator by stimulating the rate of IPNS which prevents the accumulation of LLD-ACV. As a consequence...

  12. Preliminary analysis of mortality associated with rituximab use in autoimmune diseases.

    Science.gov (United States)

    Shetty, Shawn; Ahmed, A R

    2013-12-01

    Normal antibodies and pathogenic autoantibodies are produced by B-cells and plasma cells. Rituximab is a chimeric monoclonal antibody that targets the CD20 molecule on cells that express them on their surface and kills them. Rituximab has been increasingly used to treat several autoimmune diseases. Studies on fatal outcomes associated with rituximab therapy are lacking. A comprehensive and detailed analysis in which the multiple factors that could contribute to a fatal outcome in all the autoimmune diseases in which rituximab has been used would be cumbersome, lack uniformity and would prove difficult in making certain definitive conclusions and comparisons, but more importantly it would not allow to provide specific precautions and recommendations to prevent mortality. Hence, autoimmune mucocutaneous blistering diseases (AMBD) were used as model to study fatal outcomes in patients treated with rituximab between 2000 and 2013, using uniform 13 criteria. Fatal outcomes were found in 14 patients with autoimmune blistering diseases out of 134 patients (10.4%). Patients died due to infections (75%), gastrointestinal (17%) and cardiac events (8%). Causes of death were reported in 101 patients with other autoimmune diseases out of 4320 with a mortality rate of 2.4%. Among them, 44 patients (43.6%) died from infections. A statistical analysis of the data demonstrated that a statistically significant higher mortality rate was observed in patients with AMBD compared to patients with other autoimmune diseases. Similarly, a statistically significant higher rate of death due to infections was reported in patients with AMBD compared to patients with other autoimmune diseases. Use of systemic corticosteroids and immunosuppressive agents as concomitant therapy with rituximab enhanced immunosuppression. In many patients, B-cells were depleted for prolonged periods, even after clinical recovery was observed. Although its main action is depletion of B-cells, rituximab has a

  13. Preliminary Analysis of Ground-based Orbit Determination Accuracy for the Wide Field Infrared Survey Telescope (WFIRST)

    Science.gov (United States)

    Sease, Brad

    2017-01-01

    The Wide Field Infrared Survey Telescope is a 2.4-meter telescope planned for launch to the Sun-Earth L2 point in 2026. This paper details a preliminary study of the achievable accuracy for WFIRST from ground-based orbit determination routines. The analysis here is divided into two segments. First, a linear covariance analysis of early mission and routine operations provides an estimate of the tracking schedule required to meet mission requirements. Second, a simulated operations scenario gives insight into the expected behavior of a daily Extended Kalman Filter orbit estimate over the first mission year given a variety of potential momentum unloading schemes.

  14. Development and Sensitivity Analysis of a Fully Kinetic Model of Sequential Reductive Dechlorination in Groundwater

    DEFF Research Database (Denmark)

    Malaguerra, Flavio; Chambon, Julie Claire Claudia; Bjerg, Poul Løgstrup

    2011-01-01

    been modeled using modified Michaelis–Menten kinetics and has been implemented in the geochemical code PHREEQC. The model have been calibrated using a Shuffled Complex Evolution Metropolis algorithm to observations of chlorinated solvents, organic acids, and H2 concentrations in laboratory batch...

  15. A critical analysis of kinetic data of 3-hexulosephosphate synthases. Michaelis-Menten or complex characteristics.

    Science.gov (United States)

    Müller, R; Babel, W

    1980-01-01

    Investigations of the 3-hexulosephosphate synthase (HPS) from different methylotrophic bacteria have revealed apparent discrepancies in kinetic behaviour. In all methanol-utilizing species investigated by us the kinetic characteristics showed intermediary plateau regions. Therefore, this behaviour is assumed to be a general feature of the HPS from all non-methane-utilizing methylotrophic bacteria. However, this assumption is in contrast to the results of other authors. Both for Methylomonas M15 (SAHM et al. 1976) and Methylomonas aminofaciens 77a (KATO et al. 1977, 1978) MICHAELIS-MENTEN kinetics of the HPS were stated. To check the validity of our assumption we have analyzed the kinetic data given by others. Indications of the existence of intermediary plateau regions could be found with the enzyme from Arthrobacter globiformis (BYKOVSKAYA and VORONKOV 1977) and Methylomonas aminofaciens 77a (KATO et al. 1978). Furthermore, biphasic ARRHENIUS plots indicate a multiple character of the HPS from these species as could already be demonstrated with the enzyme from Bacterium MB 58 and Pseudomonas oleovorans. In addition, causes which may obscure the detection of intermediary plateau regions are demonstrated.

  16. Formation of distinct soluble microbial products by activated sludge: kinetic analysis and quantitative determination.

    Science.gov (United States)

    Ni, Bing-Jie; Fang, Fang; Xie, Wen-Ming; Xu, Juan; Yu, Han-Qing

    2012-02-07

    Soluble microbial products (SMP) released by microorganisms in bioreactors are classified into two distinct groups according to their different chemical and degradation kinetics: utilization-associated products (UAP) and biomass-associated products (BAP). SMP are responsible for effluent chemical oxygen demand or for membrane fouling of membrane bioreactor. Here an effective and convenient approach, other than the complicated chemical methods or complex models, is developed to quantify the formation of UAP and BAP together with their kinetics in activated sludge process. In this approach, an integrated substrate utilization equation is developed and used to determine UAP and their production kinetics. On the basis of total SMP measurements, BAP formation is determined with an integrated BAP formation equation. The fraction of substrate electrons diverted to UAP, and the content of BAP derived from biomass can then be calculated. Dynamic quantification data are obtained for UAP and BAP separately and conveniently. The obtained kinetic parameters are found to be reasonable as they are generally bounded and comparable to the literature values. The validity of this approach is confirmed by independent SMP production tests in six different activated sludge systems, which demonstrates its applicability in a wide range of engineered system regarding SMP production. This work provides a widely applied approach to determine the formation of UAP and BAP conveniently, which may offer engineers with basis to optimize bioreactor operation to avoid a high effluent soluble organics from SMP or SMP-based membrane fouling in membrane bioreactors.

  17. Thermochemistry and micro-kinetic analysis of methanol synthesis on ZnO (0001)

    DEFF Research Database (Denmark)

    Medford, Andrew James; Sehested, Jens; Rossmeisl, Jan

    2014-01-01

    In this work, we examine the thermochemistry of methanol synthesis intermediates using density functional theory (DFT) and analyze the methanol synthesis reaction network using a steady-state micro-kinetic model. The energetics for methanol synthesis over Zn-terminated ZnO (0001) are obtained fro...

  18. Analysis of Ankle Kinetics during Walking in Individuals with Down Syndrome.

    Science.gov (United States)

    Cioni, Matteo; Cocilovo, Anna; Rossi, Fabio; Paci, Domenico; Valle, Maria Stella

    2001-01-01

    The biomechanical characteristics of the ankle during gait of 17 participants with Down syndrome (ages 8-36) were investigated. Participants showed significant decreases of plantar-flexor moments and of A1 and A2 joint powers. Correlation between kinetic and temporal spatial parameters was markedly reduced or weak in comparison to 10 controls.…

  19. Investigation of the Photocatalytic Activity of ZnO Nanowires: Substrate Effect and Kinetics Analysis

    Directory of Open Access Journals (Sweden)

    Jan-Hau Chang

    2014-01-01

    Full Text Available The photocatalytic activity of substrate-supported ZnO nanowires is investigated with a focus on the substrate effect and the photodegradation kinetics. The employed NWs are grown on various substrates by thermal evaporation and a 50 μM rhodamine B solution is used as the target solution. It is found that the photocatalytic activity is facilitated by using a conductive substrate and such a result can be reasonably attributed to the improvement of charge separation. The photodegradation is found to follow a zeroth-order kinetics at high concentrations and a first-order kinetics at low concentrations with a transition at around 10 μM. The present observation is opposed to the commonly accepted first-order kinetics. Degradation constants of 0.58 μM·min−1 for the zeroth-order and 0.028 min−1 for the first-order have been achieved. The first-order constant also compares favorably with reported values.

  20. Tyrosinase kinetics in epidermal melanocytes: analysis of DAG-PKC-dependent signaling pathway

    Science.gov (United States)

    Stolnitz, Mikhail M.; Peshkova, Anna Y.

    2001-05-01

    Tyrosinase is the key enzyme of melanogenesis with unusual enzyme kinetics. Protein kinase C plays an important role in regulating of tyrosinase activity. In the paper the mathematical model of PKC-DAG-dependent signal transduction pathway for UV-radiation is presented.