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Sample records for pregnane called diginane

  1. A New Pregnane Glycoside from Fermented Leaves of Agave americana

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A new minor pregnane glycoside was isolated from the fermented leaves of Agave americana. Its structure was elucidated as (20S)-5α-pregnane-3β, 20-diol 20-O-β-D-glucopyrano- side (1) by spectral methods.

  2. New Pregnane Glycosides from Gymnema sylvestre

    OpenAIRE

    Rui Xu; Yu Yang; Yang Zhang; Fengxia Ren; Jinlong Xu; Nengjiang Yu; Yimin Zhao

    2015-01-01

    Four new pregnane glycosides 1–4 were isolated from the ethanol extract of the stem of Gymnema sylvestre and named gymsylvestrosides A–D. Hydrolysis of compound 1 under the catalysis of Aspergilus niger β-glucosidase afforded compound 5 (gymsylvestroside E). Their structures were determined by spectroscopic methods such as HRESIMS, 1D and 2D NMR, as well as HMQC-TOCSY experiment. Compounds 1–4 were screened for Saccharomyces cerevisiae α-glucosidase inhibitory activity.

  3. New pregnane glycosides from Gymnema sylvestre.

    Science.gov (United States)

    Xu, Rui; Yang, Yu; Zhang, Yang; Ren, Fengxia; Xu, Jinlong; Yu, Nengjiang; Zhao, Yimin

    2015-02-12

    Four new pregnane glycosides 1-4 were isolated from the ethanol extract of the stem of Gymnema sylvestre and named gymsylvestrosides A-D. Hydrolysis of compound 1 under the catalysis of Aspergilus niger β-glucosidase afforded compound 5 (gymsylvestroside E). Their structures were determined by spectroscopic methods such as HRESIMS, 1D and 2D NMR, as well as HMQC-TOCSY experiment. Compounds 1-4 were screened for Saccharomyces cerevisiae α-glucosidase inhibitory activity.

  4. New Pregnane Glycosides from Gymnema sylvestre

    Directory of Open Access Journals (Sweden)

    Rui Xu

    2015-02-01

    Full Text Available Four new pregnane glycosides 1–4 were isolated from the ethanol extract of the stem of Gymnema sylvestre and named gymsylvestrosides A–D. Hydrolysis of compound 1 under the catalysis of Aspergilus niger β-glucosidase afforded compound 5 (gymsylvestroside E. Their structures were determined by spectroscopic methods such as HRESIMS, 1D and 2D NMR, as well as HMQC-TOCSY experiment. Compounds 1–4 were screened for Saccharomyces cerevisiae α-glucosidase inhibitory activity.

  5. New pregnane and phenolic glycosides from Solenostemma argel.

    Science.gov (United States)

    Ounaissia, Karima; Pertuit, David; Mitaine-Offer, Anne-Claire; Miyamoto, Tomofumi; Tanaka, Chiaki; Delemasure, Stéphanie; Dutartre, Patrick; Smati, Dalila; Lacaille-Dubois, Marie-Aleth

    2016-10-01

    From the aerial parts, pericarps and roots of Solenostemma argel, three new pregnane glycosides (1-3) with two known ones and a new phenolic glycoside (4) have been isolated. Their structures were established by extensive 1D - and 2D NMR and mass spectroscopic analysis. The cytotoxicity of all compounds was evaluated against two human tumor cell lines (SW 480, MCF-7), but none of them was active in the concentration range 0.9-59.0μM. Compounds 2 and the known argeloside F at non toxic concentrations for the PBMCs (27.3μM and 27.6μM, respectively) significantly decreased the Il-1β production by LPS-stimulated PBMCs. All isolated compounds showed a significant antioxidant potential with ORAC values in the concentration range 3481-9617μmoleq. Trolox/100g. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. Tanshinone IIA ameliorates dextran sulfate sodium-induced inflammatory bowel disease via the pregnane X receptor

    Directory of Open Access Journals (Sweden)

    Zhang X

    2015-12-01

    Full Text Available Xianxie Zhang,1 Yuguang Wang,2 Zengchun Ma,2 Qiande Liang,2 Xianglin Tang,2 Donghua Hu,2 Hongling Tan,2 Chengrong Xiao,2 Yue Gao2 1Air Force General Hospital of People’s Liberation Army, Beijing, People’s Republic of China; 2Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, Beijing, People’s Republic of China Abstract: Tanshinone IIA (Tan IIA (C19H18O3 is one of the major active lipophilic components in a conventional Chinese medicine called danshen, and it has long been used in the People’s Republic of China and other neighboring countries to treat patients suffering from inflammatory bowel disease (IBD. Previous experiments by many teams determined which mechanism of Tan IIA is relevant to the treatment of IBD associated with inflammation and the pregnane X receptor (PXR. The current study demonstrated that Tan IIA is an efficacious PXR agonist and its ability to induce CYP3A4 mRNA and protein expression was mediated by the transactivation of PXR, a known target of abrogating inflammation in IBD. Clinical symptoms in mice and histological assessment data suggested that administration of Tan IIA in mice demonstrated significant protection and showed that in DSS-induced IBD it acts in a concentration-dependent manner. PXR-silenced mice treated with Tan IIA demonstrated low protection against DSS-induced mouse IBD and exacerbated the severity of IBD compared with wild-type mice; PXR-silenced mice demonstrated the necessity for PXR in Tan IIA-mediated upregulation of xenobiotic metabolism genes. The IBD treatment effects of Tan IIA are partially due to PXR-mediated upregulation of xenobiotic metabolism and downregulation of inflammatory mediators. The novel findings reported here may contribute to the effective utilization of Tan IIA and its derivatives as a PXR ligand in the treatment of human IBD. This suggests that Tan IIA may have considerable clinical utility. Keywords: dashen, ulcerative colitis

  7. Synthesis and pharmacological evaluation of new 16-methyl pregnane derivatives.

    Science.gov (United States)

    Ramirez, Elena; Cabeza, Marisa; Heuze, Ivonne; Gutiérrez, Edgar; Bratoeff, Eugene; Membrillo, Marisol; Lira, Alfonso

    2002-01-01

    The pharmacological activity of several new pregnane derivatives 15-19 were determined on gonadectomized male hamster flank organs, seminal vesicles and in vitro conversion of testosterone (T) to dihydrotestosterone (DHT) as 5alpha-reductase inhibitors. Steroids 15-19 decreased the diameter of the pigmented spot in the flank organs as compared to the T treated animals; in this model, steroids 16 and 19 showed a higher activity than the commercially available finasteride 3. Injection of T increased the weight of the seminal vesicles. Compounds 15-19 when injected together with T decreased the weight of the seminal vesicles thus showing an antiandrogenic effect. The trienone 19 exhibited a considerably higher activity than finasteride. Steroids 15-19 inhibited the in vitro metabolism of [3H]T to [3H]DHT in seminal vesicles homogenates of gonadectomized male hamsters. Compounds 18 and 19 showed a much higher antiandrogenic effect than finasteride. This enhancement of the biological activity could probably be attributed to the coplanarity of the steroidal skeleton as previously observed by our group. The high antiandrogenic activity of the epoxy compound 16 is probably the result of the ring opening of the oxiran ring with the nucleophilic part of the enzyme 5alpha-reductase thus leading to a stable adduct with concomitant deactivation of this enzyme.

  8. Pregnane X Receptor and Cancer: Context-Specificity is Key

    Science.gov (United States)

    Pondugula, Satyanarayana R.; Pavek, Petr; Mani, Sridhar

    2016-01-01

    Pregnane X receptor (PXR) is an adopted orphan nuclear receptor that is activated by a wide-range of endobiotics and xenobiotics, including chemotherapy drugs. PXR plays a major role in the metabolism and clearance of xenobiotics and endobiotics in liver and intestine via induction of drug-metabolizing enzymes and drug-transporting proteins. However, PXR is expressed in several cancer tissues and the accumulating evidence strongly points to the differential role of PXR in cancer growth and progression as well as in chemotherapy outcome. In cancer cells, besides regulating the gene expression of enzymes and proteins involved in drug metabolism and transport, PXR also regulates other genes involved in proliferation, metastasis, apoptosis, anti-apoptosis, inflammation, and oxidative stress. In this review, we focus on the differential role of PXR in a variety of cancers, including prostate, breast, ovarian, endometrial, and colon. We also discuss the future directions to further understand the differential role of PXR in cancer, and conclude with the need to identify novel selective PXR modulators to target PXR in PXR-expressing cancers. PMID:27617265

  9. Pregnane X Receptor and Cancer: Context-Specificity is Key

    Directory of Open Access Journals (Sweden)

    Satyanarayana R. Pondugula

    2016-06-01

    Full Text Available Pregnane X receptor (PXR is an adopted orphan nuclear receptor that is activated by a wide-range of endobiotics and xenobiotics, including chemotherapy drugs. PXR plays a major role in the metabolism and clearance of xenobiotics and endobiotics in liver and intestine via induction of drug-metabolizing enzymes and drug-transporting proteins. However, PXR is expressed in several cancer tissues and the accumulating evidence strongly points to the differential role of PXR in cancer growth and progression as well as in chemotherapy outcome. In cancer cells, besides regulating the gene expression of enzymes and proteins involved in drug metabolism and transport, PXR also regulates other genes involved in proliferation, metastasis, apoptosis, anti-apoptosis, inflammation, and oxidative stress. In this review, we focus on the differential role of PXR in a variety of cancers, including prostate, breast, ovarian, endometrial, and colon. We also discuss the future directions to further understand the differential role of PXR in cancer, and conclude with the need to identify novel selective PXR modulators to target PXR in PXR-expressing cancers.

  10. Diethylstilbestrol-scaffold-based pregnane X receptor modulators.

    Science.gov (United States)

    Hodnik, Žiga; Tomašič, Tihomir; Smodiš, Domen; D'Amore, Claudio; Mašič, Lucija Peterlin; Fiorucci, Stefano; Kikelj, Danijel

    2015-10-20

    Due to its function as a regulator of drug-metabolizing enzymes and transporters, pregnane X receptor (PXR) represents an important factor involved in drug metabolism. In this work, we describe the discovery of diethylstilbestrol-based PXR modulators, which were designed from marine sulfated steroids with PXR agonistic activity, solomonsterols A and B, and our recently reported bazedoxifene scaffold-derived PXR antagonists. The methylated diethylstilbestrol derivative 1 displayed potent PXR agonistic activity with an EC50 value of 10.5 μM, whereas compounds 3, 4 and 6 (IC50 for 6 = 27.4 μM) and diethylstilbestrol (2) itself (IC50 = 14.6 μM) exhibited PXR antagonistic effects in HepG2 cells. The PXR modulatory effects of the synthesized diethylstilbestrol derivatives were further confirmed by the induction of PXR-regulated CYP3A4 expression with compound 1, as well as by the inhibition of the rifaximin-promoted up-regulation of CYP3A4 expression with 2 and its derivative 6.

  11. Pregnane X receptor: a double-edged sword

    Institute of Scientific and Technical Information of China (English)

    FANG Dao-kui; ZHANG Jian-qing

    2009-01-01

    @@ During the last decade, much progress has been made in exploring the mechanisms of alterations elicited by foreign compounds in xeno- and endobiotic metabolism regulated by the human nuclear pregnane X receptor (PXR). PXR, identified as a human nuclear receptor in 1998 and generally regarded as a sensor activated by exogenous and endogenous chemicals,regulates a large number of enzymes and transporters involved in the response of mammals to their chemical environment) PXR activation is ligand dependent.Following ligand binding, PXR forms a heterodimer with the retinoid X receptor (RXR) that binds to PXR response dements, resulting in their transcriptional activation. PXR is mainly associated with the cellular response to xenobiotics, including induction of enzymes involved in drug oxidation and conjugation, as well as induction of xenobiotic and endobiotic transporters. Unlike other nuclear receptors such as the steroid receptors that interact selectively with their physiological ligands, PXR ligands are structurally diverse and include prescription drugs,herbal medicines, dietary supplements, environmental pollutants, and endobiotics. PXR exhibits the ability to bind multiple ligands, and each receptor's ligand specificity is species-dependent. The detailed information of PXR was summarized (Table 1). The purpose of this review is to highlight the promiscuous ligand-binding properties of PXR, its positive effect in regulation of the body's garbage-disposal system and negative effect in harmful drug-drug interactions and endocrine disruption.

  12. Identification of Gene Markers for Activation of the Nuclear Receptor Pregnane X Receptor

    Science.gov (United States)

    Many environmentally-relevant chemicals and drugs activate the nuclear receptor pregnane X receptor (PXR). Activation of PXR in the mouse liver can lead to increases in liver weight in part through increased hepatocyte replication similar to chemicals that activate other nuclear ...

  13. Identification of Gene Markers for Activation of the Nuclear Receptor Pregnane X Receptor

    Science.gov (United States)

    Many environmentally-relevant chemicals and drugs activate the nuclear receptor pregnane X receptor (PXR). Activation of PXR in the mouse liver can lead to increases in liver weight in part through increased hepatocyte replication similar to chemicals that activate other nuclear ...

  14. EMERGENCY CALLS

    CERN Multimedia

    2001-01-01

    IN URGENT NEED OF A DOCTOR GENEVA EMERGENCY SERVICES GENEVA AND VAUD 144 FIRE BRIGADE 118 POLICE 117 CERN FIREMEN 767-44-44 ANTI-POISONS CENTRE Open 24h/24h 01-251-51-51 Patient not fit to be moved, call family doctor, or: GP AT HOME, open 24h/24h 748-49-50 Association Of Geneva Doctors Emergency Doctors at home 07h-23h 322 20 20 Patient fit to be moved: HOPITAL CANTONAL CENTRAL 24 Micheli-du-Crest 372-33-11 ou 382-33-11 EMERGENCIES 382-33-11 ou 372-33-11 CHILDREN'S HOSPITAL 6 rue Willy-Donzé 372-33-11 MATERNITY 32 bvd.de la Cluse 382-68-16 ou 382-33-11 OPHTHALMOLOGY 22 Alcide Jentzer 382-33-11 ou 372-33-11 MEDICAL CENTRE CORNAVIN 1-3 rue du Jura 345 45 50 HOPITAL DE LA TOUR Meyrin EMERGENCIES 719-61-11 URGENCES PEDIATRIQUES 719-61-00 LA TOUR MEDICAL CENTRE 719-74-00 European Emergency Call 112 FRANCE EMERGENCY SERVICES 15 FIRE BRIGADE 18 POLICE 17 CERN FIREMEN AT HOME 00-41-22-767-44-44 ANTI-POISONS CENTRE Open 24h/24h 04-72-11-69-11 All doctors ...

  15. Chronic Exposure to Rifaximin Causes Hepatic Steatosis in Pregnane X Receptor-Humanized Mice

    OpenAIRE

    Cheng, Jie; Krausz, Kristopher W.; Tanaka, Naoki; Gonzalez, Frank

    2012-01-01

    Rifaximin, a nonsystemic antibiotic that exhibits low gastrointestinal absorption, is a potent agonist of human pregnane X receptor (PXR), which contributes to its therapeutic efficacy in inflammatory bowel disease. To investigate the effects of long-term administration of rifaximin on the liver, PXR-humanized mice were administered rifaximin for 6 months; wild-type and Pxr-null mice were treated in parallel as controls. Histological analysis revealed time-dependent intense hepatocellular fat...

  16. Therapeutic Role of Rifaximin in Inflammatory Bowel Disease: Clinical Implication of Human Pregnane X Receptor Activation

    OpenAIRE

    Cheng, Jie; Yatrik M. Shah; Ma, Xiaochao; Pang, Xiaoyan; Tanaka, Toshiya; Kodama, Tatsuhiko; Krausz, Kristopher W.; Gonzalez, Frank J.

    2010-01-01

    Human pregnane X receptor (PXR) has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Rifaximin, a human PXR activator, is in clinical trials for treatment of IBD and has demonstrated efficacy in Crohn's disease and active ulcerative colitis. In the current study, the protective and therapeutic role of rifaximin in IBD and its respective mechanism were investigated. PXR-humanized (hPXR), wild-type, and Pxr-null mice were treated with rifaximin in the dextran sulfate sod...

  17. Pregnane X receptor and natural products: beyond drug–drug interactions

    OpenAIRE

    Staudinger, Jeff L.; Ding, Xunshan; Lichti, Kristin

    2006-01-01

    The pregnane X receptor (PXR, NR1I2) is a member of the nuclear receptor superfamily that is activated by a myriad of compounds and natural products in clinical use. Activation of PXR represents the basis for several clinically important drug–drug interactions. Although PXR activation has undesirable effects in patients on combination therapy, it also mediates the hepatoprotective effects exhibited by some herbal remedies. This review focuses on PXR activation by natural products and the pote...

  18. Marine and semi-synthetic hydroxysteroids as new scaffolds for pregnane x receptor modulation.

    Digital Repository Service at National Institute of Oceanography (India)

    Sepe, V.; DiLeva, F.S.; D'Amore, C.; Festa, C.; DeMarino, S.; Renga, B.; D’Auria, M.V.; Novellino, E.; Limongelli, V.; DeSouza, L.; Majik, M.; Zampella, A.; Fiorucci, S.

    stream_size 73525 stream_content_type text/plain stream_name Mar_Drugs_12_3091.pdf.txt stream_source_info Mar_Drugs_12_3091.pdf.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 Mar. Drugs 2014, 12, 3091...); hydroxysteroids; docking simulations 1. Introduction The pregnane X receptor (PXR, NR1I2) belongs to the nuclear receptor (NR) family and is well recognized for its pivotal role as a ―xenobiotic sensor‖ that transcriptionally regulates the expression...

  19. (20S-20-Acetamido-18-chloro-5α-pregnan-3β-yl acetate

    Directory of Open Access Journals (Sweden)

    Michael Benn

    2010-04-01

    Full Text Available In the title compound, C25H40ClNO3, prepared by the thermolysis of (20S-O,N-diacetyl-20-amino-N-chloro-3β-hydroxy-5α-pregnane, the three six-membered rings adopt chair conformations while the five-membered ring is in an envelope conformation. The ester group attached to ring A is in an equatorial position. All the rings are trans-fused. Intramolecular C—H...O and C—H...Cl interactions occur. The crystal structure is stabilized by intermolecular N—H...O and C—H...O interactions close contacts occur.

  20. Pregnane X receptor as a target for treatment of inflammatory bowel disorders

    OpenAIRE

    Cheng, Jie; Yatrik M. Shah; Gonzalez, Frank J.

    2012-01-01

    Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, has a major role in the induction of genes involved in drug transport and metabolism. Recent studies in mice have provided insight into a novel function for PXR in inflammatory bowel disease (IBD). The mechanisms of the protective effect of PXR activation on IBD is not fully established, but is due in part to the attenuation of nuclear factor kappa B (NF-κB) signaling that results in lower expression of proinflamm...

  1. Pregnane-Type Steroids from the Formosan Soft Coral Scleronephthya flexilis

    Directory of Open Access Journals (Sweden)

    Chao-Ying Kuo

    2014-06-01

    Full Text Available Three pregnane-type steroids, including a new metabolite, 3β-methoxy-5,20-pregnadiene (1 along with two known analogues, 3β-acetoxy-5,20-pregnadiene (2 and 5α-pregna-1,20-dien-3-one (3 were isolated from the soft coral Scleronephthya flexilis. Standard spectroscopic techniques were used to determine the structure of new steroid 1. The absolute stereochemistry of steroid 2 was confirmed by X-ray diffraction analysis. Steroid 3 exhibited potent activity against MOLT-4 tumor cells.

  2. Verticillosides A-M: Polyoxygenated pregnane glycosides from Asclepias verticillata L.

    Science.gov (United States)

    Araya, Juan J; Binns, Franklin; Kindscher, Kelly; Timmermann, Barbara N

    2012-06-01

    As part of our ongoing effort to explore the chemical diversity of plants of the United States Midwest region, the isolation and identification of 13 pregnane glycosides named verticillosides A-M from Asclepias verticillata L. are reported. The structures of these compounds were elucidated by various spectroscopic techniques, including 1D and 2D NMR, IR, UV, and HRMS. The cytotoxicity of the isolates was evaluated against paired breast cell lines Hs578T (cancer) and Hs578Bst (normal), however, no significant growth inhibition was observed.

  3. Cytotoxic pregnane steroids from the seeds of Cipadessa baccifera (Roth.) Miq.

    Science.gov (United States)

    Zhao, Lei; Zhang, Ping; Su, Xiao-Jie; Zhang, Bing

    2017-03-01

    A chemical investigation of the 80% ethanol extract of the seeds of Cipadessa baccifera (Roth.) Miq. led to the isolation of five new pregnane steroids, 17α,18,20S-trihydroxy-pregn-4-en-3,16-dione (1), 18-hydoxy-pregn-4,17(20)-trans-dien-3,16-dione (2), 3β,18-dihydroxy-pregn-5,17(20)-trans-dien-16-one (3), 2α,3β,4β,18-tetrahydroxy-pregn-5,17(20)-trans-dien-16-one (4), and 2α,3β,4β,17α,18,20S-hexahydroxy-pregn-5-en-16-one (5), along with two known compounds, 17α,20S-dihydroxy-pregn-4-en-3,16-dione (6) and 3β-hydroxy-pregn-5,17-dien-16-one (7). Structural elucidation of all the compounds was accomplished by spectral methods such as 1D and 2D NMR, IR, UV, and HRESIMS. The isolated compounds were tested in vitro for cytotoxic activities against seven tumor cell lines. As a result, pregnane-type steroids 1, 5 and 6 exhibited cytotoxicity with IC50 values <20μM against all tested tumor cell lines except meningioma cells (BEN-MEN-1).

  4. Acetylation of pregnane X receptor protein determines selective function independent of ligand activation

    Energy Technology Data Exchange (ETDEWEB)

    Biswas, Arunima; Pasquel, Danielle [Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461 (United States); Tyagi, Rakesh Kumar [Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067 (India); Mani, Sridhar, E-mail: sridhar.mani@einstein.yu.edu [Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461 (United States)

    2011-03-18

    Research highlights: {yields} Pregnane X receptor (PXR), a major regulatory protein, is modified by acetylation. {yields} PXR undergoes dynamic deacetylation upon ligand-mediated activation. {yields} SIRT1 partially mediates PXR deacetylation. {yields} PXR deacetylation per se induces lipogenesis mimicking ligand-mediated activation. -- Abstract: Pregnane X receptor (PXR), like other members of its class of nuclear receptors, undergoes post-translational modification [PTM] (e.g., phosphorylation). However, it is unknown if acetylation (a major and common form of protein PTM) is observed on PXR and, if it is, whether it is of functional consequence. PXR has recently emerged as an important regulatory protein with multiple ligand-dependent functions. In the present work we show that PXR is indeed acetylated in vivo. SIRT1 (Sirtuin 1), a NAD-dependent class III histone deacetylase and a member of the sirtuin family of proteins, partially mediates deacetylation of PXR. Most importantly, the acetylation status of PXR regulates its selective function independent of ligand activation.

  5. Call Forecasting for Inbound Call Center

    Directory of Open Access Journals (Sweden)

    Peter Vinje

    2009-01-01

    Full Text Available In a scenario of inbound call center customer service, the ability to forecast calls is a key element and advantage. By forecasting the correct number of calls a company can predict staffing needs, meet service level requirements, improve customer satisfaction, and benefit from many other optimizations. This project will show how elementary statistics can be used to predict calls for a specific company, forecast the rate at which calls are increasing/decreasing, and determine if the calls may stop at some point.

  6. Marine and Semi-Synthetic Hydroxysteroids as New Scaffolds for Pregnane X Receptor Modulation

    Directory of Open Access Journals (Sweden)

    Valentina Sepe

    2014-05-01

    Full Text Available In recent years many sterols with unusual structures and promising biological profiles have been identified from marine sources. Here we report the isolation of a series of 24-alkylated-hydroxysteroids from the soft coral Sinularia kavarattiensis, acting as pregnane X receptor (PXR modulators. Starting from this scaffold a number of derivatives were prepared and evaluated for their ability to activate the PXR by assessing transactivation and quantifying gene expression. Our study reveals that ergost-5-en-3β-ol (4 induces PXR transactivation in HepG2 cells and stimulates the expression of the PXR target gene CYP3A4. To shed light on the molecular basis of the interaction between these ligands and PXR, we investigated, through docking simulations, the binding mechanism of the most potent compound of the series, 4, to the PXR. Our findings provide useful functional and structural information to guide further investigations and drug design.

  7. Marine and semi-synthetic hydroxysteroids as new scaffolds for pregnane X receptor modulation.

    Science.gov (United States)

    Sepe, Valentina; Di Leva, Francesco Saverio; D'Amore, Claudio; Festa, Carmen; De Marino, Simona; Renga, Barbara; D'Auria, Maria Valeria; Novellino, Ettore; Limongelli, Vittorio; D'Souza, Lisette; Majik, Mahesh; Zampella, Angela; Fiorucci, Stefano

    2014-05-27

    In recent years many sterols with unusual structures and promising biological profiles have been identified from marine sources. Here we report the isolation of a series of 24-alkylated-hydroxysteroids from the soft coral Sinularia kavarattiensis, acting as pregnane X receptor (PXR) modulators. Starting from this scaffold a number of derivatives were prepared and evaluated for their ability to activate the PXR by assessing transactivation and quantifying gene expression. Our study reveals that ergost-5-en-3β-ol (4) induces PXR transactivation in HepG2 cells and stimulates the expression of the PXR target gene CYP3A4. To shed light on the molecular basis of the interaction between these ligands and PXR, we investigated, through docking simulations, the binding mechanism of the most potent compound of the series, 4, to the PXR. Our findings provide useful functional and structural information to guide further investigations and drug design.

  8. Nizwaside: a new anticancer pregnane glycoside from the sap of Desmidorchis flava.

    Science.gov (United States)

    Hussain, Hidayat; Raees, Muhammad Adil; Rehman, Najeeb Ur; Al-Rawahi, Ahmed; Csuk, René; Khan, Husain Yar; Abbas, Ghulam; Al-Broumi, Mohammed Abdullah; Green, Ivan R; Elyassi, Ali; Mahmood, Talat; Al-Harrasi, Ahmed

    2015-12-01

    The sap from the succulent Desmidorchis flava (N.E.Br) Meve and Liede yielded a new pregnane glycoside, named nizwaside whose structure was established using 1D and 2D NMR techniques as well as mass spectrometry (ESIMS). Nizwaside was tested for anticancer, DPPH antioxidant, urease enzyme inhibition, α-glucosidase enzyme inhibition and acetylcholinesterase inhibition activities. Interestingly, nizwaside showed significant anti-proliferative effects on MDA MB231 breast cancer cells with an IC(50) of 23.5 µg/ml. Moreover, nizwaside was more effective than Doxorubicin, a well-known clinical anticancer drug, in suppressing MDA MB231 cell proliferation even at concentrations lower than that of Doxorubicin (75 µg/ml nizwaside vs. 100 µg/ml Doxorubicin). On the other hand, nizwaside showed relatively weak antioxidant activity with 15 % inhibition.

  9. Pregnane X receptor as a target for treatment of inflammatory bowel disorders.

    Science.gov (United States)

    Cheng, Jie; Shah, Yatrik M; Gonzalez, Frank J

    2012-06-01

    Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, has a major role in the induction of genes involved in drug transport and metabolism. Recent studies in mice have provided insight into a novel function for PXR in inflammatory bowel disease (IBD). The mechanism of the protective effect of PXR activation on IBD is not fully established, but is due in part to the attenuation of nuclear factor (NF)-κB signaling that results in lower expression of proinflammatory cytokines. Recent clinical trials with the antibiotic rifaximin, a PXR agonist in the gastrointestinal system, have revealed its potential therapeutic value in the treatment of intestinal inflammation in humans. Thus, PXR may be a novel target for IBD therapy.

  10. Bioactive Pregnane Steroids from a South China Sea Gorgonian Carijoa sp.

    Directory of Open Access Journals (Sweden)

    Fei Cao

    2013-03-01

    Full Text Available A new pregnane steroid, 1, and three known analogues 2–4, have been isolated from a gorgonian Carijoa sp. collected from the South China Sea. The planar structure and relative configuration of 1 were elucidated from comprehensive spectroscopic data. Its absolute configuration was determined by application of the modified Mosher method. Compounds 1, 3 and 4 exhibited cytotoxicity against the human hepatoma cell line Bel-7402, with IC50 values of 9.33, 11.02 and 18.68 µM, respectively. Additionally, compound 1 exhibited promising antibacterial activity against Pseudomona puido, with a MIC value of 31 nM, which is approximately 5-fold more potent than ciprofloxacin (MIC = 156 nM.

  11. Pregnane X receptor and natural products: beyond drug-drug interactions.

    Science.gov (United States)

    Staudinger, Jeff L; Ding, Xunshan; Lichti, Kristin

    2006-12-01

    The pregnane X receptor (PXR, NR1I2) is a member of the nuclear receptor superfamily that is activated by a myriad of compounds and natural products in clinical use. Activation of PXR represents the basis for several clinically important drug-drug interactions. Although PXR activation has undesirable effects in patients on combination therapy, it also mediates the hepatoprotective effects exhibited by some herbal remedies. This review focuses on PXR activation by natural products and the potential therapeutic opportunities presented. In particular, the biological effects of St. John's Wort, gugulipid, kava kava, Coleus forskolii, Hypoxis, Sutherlandia, qing hao, wu wei zi, gan cao and other natural products are discussed. The impact of these natural products on drug metabolism and hepatoprotection is highlighted in the context of activation and antagonism of PXR.

  12. Pregnane X receptor and natural products: beyond drug–drug interactions

    Science.gov (United States)

    Staudinger, Jeff L; Ding, Xunshan; Lichti, Kristin

    2010-01-01

    The pregnane X receptor (PXR, NR1I2) is a member of the nuclear receptor superfamily that is activated by a myriad of compounds and natural products in clinical use. Activation of PXR represents the basis for several clinically important drug–drug interactions. Although PXR activation has undesirable effects in patients on combination therapy, it also mediates the hepatoprotective effects exhibited by some herbal remedies. This review focuses on PXR activation by natural products and the potential therapeutic opportunities presented. In particular, the biological effects of St. John’s Wort, gugulipid, kava kava, Coleus forskolii, Hypoxis, Sutherlandia, qing hao, wu wei zi, gan cao and other natural products are discussed. The impact of these natural products on drug metabolism and hepatoprotection is highlighted in the context of activation and antagonism of PXR. PMID:17125405

  13. Identification of new human pregnane X receptor ligands among pesticides using a stable reporter cell system.

    Science.gov (United States)

    Lemaire, Géraldine; Mnif, Wissem; Pascussi, Jean-Marc; Pillon, Arnaud; Rabenoelina, Fanja; Fenet, Hélène; Gomez, Elena; Casellas, Claude; Nicolas, Jean-Claude; Cavaillès, Vincent; Duchesne, Marie-Josèphe; Balaguer, Patrick

    2006-06-01

    Pregnane X receptor (PXR, NR1I2) is activated by various chemically unrelated compounds, including environmental pollutants and drugs. We proceeded here to in vitro screening of 28 pesticides with a new reporter system that detects human pregnane X receptor (hPXR) activators. The cell line was obtained by a two-step stable transfection of cervical cancer HeLa cells. The first transfected cell line, HG5LN, contained an integrated luciferase reporter gene under the control of a GAL4 yeast transcription factor-binding site. The second cell line HGPXR was derived from HG5LN and stably expressed hPXR ligand-binding domain fused to GAL4 DNA-binding domain (DBD). The HG5LN cells were used as a control to detect nonspecific activities. Pesticides from various chemical classes were demonstrated, for the first time, to be hPXR activators: (1) herbicides: pretilachlor, metolachlor, and alachlor chloracetanilides, oxadiazon oxiconazole, and isoproturon urea; (2) fungicides: bupirimate and fenarimol pyrimidines, propiconazole, fenbuconazole, prochloraz conazoles, and imazalil triazole; and (3) insecticides: toxaphene organochlorine, permethrin pyrethroid, fipronil pyrazole, and diflubenzuron urea. Pretilachlor, metolachlor, bupirimate, and oxadiazon had an affinity for hPXR equal to or greater than the positive control rifampicin. Some of the newly identified hPXR activators were also checked for their ability to induce cytochrome P450 3A4 expression in a primary culture of human hepatocytes. HGPXR, with HG5LN as a reference, was grafted onto nude mice to assess compound bioavailability through in vivo quantification of hPXR activation. Altogether, our data indicate that HGPXR cells are an efficient tool for identifying hPXR ligands and establishing pesticides as hPXR activators.

  14. Fecal 20-oxo-pregnane concentrations in free-ranging African elephants (Loxodonta africana) treated with porcine zona pellucida vaccine.

    Science.gov (United States)

    Ahlers, M J; Ganswindt, A; Münscher, S; Bertschinger, H J

    2012-07-01

    Because of overpopulation of African elephants in South Africa and the consequent threat to biodiversity, the need for a method of population control has become evident. In this regard, the potential use of the porcine zona pellucida (pZP) vaccine as an effective means for population control is explored. While potential effects of pZP treatment on social behavior of African elephants have been investigated, no examination of the influence of pZP vaccination on the endocrine correlates in treated females has been undertaken. In this study, ovarian activity of free-ranging, pZP-treated African elephant females was monitored noninvasively for 1 yr at Thornybush Private Nature Reserve, South Africa, by measuring fecal 5α-pregnan-3β-ol-20-on concentrations via enzyme immunoassay. A total of 719 fecal samples from 19 individuals were collected over the study period, averaging 38 samples collected per individual (minimum, maximum: 16, 52). Simultaneously, behavioral observations were made to record the occurrence of estrous behavior for comparison. Each elephant under study showed 5α-pregnan-3β-ol-20-on concentrations rising above baseline at some period during the study indicating luteal activity. Average 5α-pregnan-3β-ol-20-on concentrations were 1.61 ± 0.46 μg/g (mean ± SD). Within sampled females, 42.9% exhibited estrous cycles within the range reported for captive African elephants, 14.3% had irregular cycles, and 42.9% did not appear to be cycling. Average estrous cycle duration was 14.72 ± 0.85 wk. Estrous behavior coincided with the onset of the luteal phase and a subsequent rise in 5α-pregnan-3β-ol-20-on concentrations. Average 5α-pregnan-3β-ol-20-on levels positively correlated with rainfall. No association between average individual 5α-pregnan-3β-ol-20-on concentrations or cyclicity status with age or parity were detected. Earlier determination of efficacy was established via fecal hormone analysis with no pregnancies determined 22 mo post

  15. Activation of Intestinal Human Pregnane X Receptor Protects against Azoxymethane/Dextran Sulfate Sodium–Induced Colon Cancer

    OpenAIRE

    Cheng, Jie; Fang, Zhong-Ze; Nagaoka, Kenjiro; Okamoto, Minoru; Qu, Aijuan; Tanaka, Naoki; Kimura, Shioko; Gonzalez, Frank J.

    2014-01-01

    The role of intestinal human pregnane X receptor (PXR) in colon cancer was determined through investigation of the chemopreventive role of rifaximin, a specific agonist of intestinal human PXR, toward azoxymethane (AOM)/dextran sulfate sodium (DSS)–induced colon cancer. Rifaximin treatment significantly decreased the number of colon tumors induced by AOM/DSS treatment in PXR-humanized mice, but not wild-type or Pxr-null mice. Additionally, rifaximin treatment markedly increased the survival r...

  16. Pregnane-X-Receptor Mediates The Anti-inflammatory Activities of Rifaximin on Detoxification Pathways in Intestinal Epithelial cells

    OpenAIRE

    Mencarelli, Andrea; Migliorati, Marco; Barbanti, Miriam; Cipriani, Sabrina; Palladino, Giuseppe; Distrutti, Eleonora; Renga, Barbara; Fiorucci, Stefano

    2010-01-01

    Abstract The pregnane-X-receptor (PXR) is master gene overseeing detoxification of wide number of xenobiotics and is critical for maintenance of intestinal integrity. The intestinal expression of genes involved in cellular detoxification is down-regulated in patients with inflammatory bowel diseases (IBD). Rifaximin, is a non absorbable antibiotic endowed with a PXR agonistic activity. In the present study we have investigated whether rifaximin activates PXR in primary human colon ...

  17. Structural Disorder in the Complex of Human Pregnane X Receptor and the Macrolide Antibiotic Rifampicin

    Energy Technology Data Exchange (ETDEWEB)

    Chrencik, Jill E.; Orans, Jillian; Moore, Linda B.; Xue, Yu; Peng, Li; Collins, Jon L.; Wisely, G. Bruce; Lambert, Millard H.; Kliewer, Steven A.; Redinbo, Matthew R. (U. of Texas-SMED); (UNC)

    2010-07-13

    The human nuclear xenobiotic receptor, pregnane X receptor (PXR), detects a variety of structurally distinct endogenous and xenobiotic compounds and controls expression of genes central to drug and cholesterol metabolism. The macrolide antibiotic rifampicin, a front-line treatment for tuberculosis, is an established PXR agonist and, at 823 Da, is one of the largest known ligands for the receptor. We present the 2.8 {angstrom} crystal structure of the ligand-binding domain of human PXR in complex with rifampicin. We also use structural and mutagenesis data to examine the origins of the directed promiscuity exhibited by the PXRs across species. Three structurally flexible loops adjacent to the ligand-binding pocket of PXR are disordered in this crystal structure, including the 200-210 region that is part of a sequence insert novel to the promiscuous PXRs relative to other members of the nuclear receptor superfamily. The 4-methyl-1-piperazinyl ring of rifampicin, which would lie adjacent to the disordered protein regions, is also disordered and not observed in the structure. Taken together, our results indicate that one wall of the PXR ligand-binding cavity can remain flexible even when the receptor is in complex with an activating ligand. These observations highlight the key role that structural flexibility plays in PXR's promiscuous response to xenobiotics.

  18. Statins Increase Plasminogen Activator Inhibitor Type 1 Gene Transcription through a Pregnane X Receptor Regulated Element.

    Directory of Open Access Journals (Sweden)

    Frederick M Stanley

    Full Text Available Plasminogen activator inhibitor type 1 (PAI-1 is a multifunctional protein that has important roles in inflammation and wound healing. Its aberrant regulation may contribute to many disease processes such as heart disease. The PAI-1 promoter is responsive to multiple inputs including cytokines, growth factors, steroids and oxidative stress. The statin drugs, atorvastatin, mevastatin and rosuvastatin, increased basal and stimulated expression of the PAI-1 promoter 3-fold. A statin-responsive, nuclear hormone response element was previously identified in the PAI-1 promoter, but it was incompletely characterized. We characterized this direct repeat (DR of AGGTCA with a 3-nucleotide spacer at -269/-255 using deletion and directed mutagenesis. Deletion or mutation of this element increased basal transcription from the promoter suggesting that it repressed PAI-1 transcription in the unliganded state. The half-site spacing and the ligand specificity suggested that this might be a pregnane X receptor (PXR responsive element. Computational molecular docking showed that atorvastatin, mevastatin and rosuvastatin were structurally compatible with the PXR ligand-binding pocket in its agonist conformation. Experiments with Gal4 DNA binding domain fusion proteins showed that Gal4-PXR was activated by statins while other DR + 3 binding nuclear receptor fusions were not. Overexpression of PXR further enhanced PAI-1 transcription in response to statins. Finally, ChIP experiments using Halo-tagged PXR and RXR demonstrated that both components of the PXR-RXR heterodimer bound to this region of the PAI-1 promoter.

  19. Pregnane X-receptor promotes stem cell-mediated colon cancer relapse

    Science.gov (United States)

    Grillet, Fanny; Finetti, Pascal; Bertucci, François; Gironella, Meritxell; Lozano, Juan José; Beucher, Bertrand; Giraud, Julie; Garambois, Véronique; Vincent, Charles; Brown, Daniel; Caillo, Ludovic; Kantar, Jovana; Pelegrin, André; Prudhomme, Michel; Ripoche, Jérémie; Bourgaux, Jean François; Ginestier, Christophe; Castells, Antoni; Hollande, Frédéric; Pannequin, Julie; Pascussi, Jean Marc

    2016-01-01

    Colorectal cancer lethality usually results from post-treatment relapse in the majority of stage II-IV patients, due to the enhanced resistance of Cancer Stem Cells (CSCs). Here, we show that the nuclear receptor Pregnane X Receptor (PXR, NR1I2), behaves as a key driver of CSC-mediated tumor recurrence. First, PXR is specifically expressed in CSCs, where it drives the expression of genes involved in self-renewal and chemoresistance. Clinically, high levels of PXR correlate with poor recurrence-free survival in a cohort of >200 stage II/III colorectal cancer patients treated with chemotherapy, for whom finding biomarkers of treatment outcome is an urgent clinical need. shRNA silencing of PXR increased the chemo-sensitivity of human colon CSCs, reduced their self-renewal and tumor-initiating potential, and drastically delayed tumor recurrence in mice following chemotherapy. This study uncovers PXR as a key factor for CSC self-renewal and chemoresistance and targeting PXR thus represents a promising strategy to minimize colorectal cancer relapse by selectively sensitizing CSCs to chemotherapy. PMID:27448961

  20. Pregnane X-receptor promotes stem cell-mediated colon cancer relapse.

    Science.gov (United States)

    Planque, Chris; Rajabi, Fatemeh; Grillet, Fanny; Finetti, Pascal; Bertucci, François; Gironella, Meritxell; Lozano, Juan José; Beucher, Bertrand; Giraud, Julie; Garambois, Véronique; Vincent, Charles; Brown, Daniel; Caillo, Ludovic; Kantar, Jovana; Pelegrin, André; Prudhomme, Michel; Ripoche, Jérémie; Bourgaux, Jean François; Ginestier, Christophe; Castells, Antoni; Hollande, Frédéric; Pannequin, Julie; Pascussi, Jean Marc

    2016-08-30

    Colorectal cancer lethality usually results from post-treatment relapse in the majority of stage II-IV patients, due to the enhanced resistance of Cancer Stem Cells (CSCs). Here, we show that the nuclear receptor Pregnane X Receptor (PXR, NR1I2), behaves as a key driver of CSC-mediated tumor recurrence. First, PXR is specifically expressed in CSCs, where it drives the expression of genes involved in self-renewal and chemoresistance. Clinically, high levels of PXR correlate with poor recurrence-free survival in a cohort of >200 stage II/III colorectal cancer patients treated with chemotherapy, for whom finding biomarkers of treatment outcome is an urgent clinical need. shRNA silencing of PXR increased the chemo-sensitivity of human colon CSCs, reduced their self-renewal and tumor-initiating potential, and drastically delayed tumor recurrence in mice following chemotherapy. This study uncovers PXR as a key factor for CSC self-renewal and chemoresistance and targeting PXR thus represents a promising strategy to minimize colorectal cancer relapse by selectively sensitizing CSCs to chemotherapy.

  1. Chronic exposure to rifaximin causes hepatic steatosis in pregnane X receptor-humanized mice.

    Science.gov (United States)

    Cheng, Jie; Krausz, Kristopher W; Tanaka, Naoki; Gonzalez, Frank J

    2012-10-01

    Rifaximin, a nonsystemic antibiotic that exhibits low gastrointestinal absorption, is a potent agonist of human pregnane X receptor (PXR), which contributes to its therapeutic efficacy in inflammatory bowel disease. To investigate the effects of long-term administration of rifaximin on the liver, PXR-humanized mice were administered rifaximin for 6 months; wild-type and Pxr-null mice were treated in parallel as controls. Histological analysis revealed time-dependent intense hepatocellular fatty degeneration and increased hepatic triglycerides in PXR-humanized mice and not in wild-type and Pxr-null mice. After long-term treatment, PXR target genes were induced in small intestine and liver, with significant up-regulation in the expression of hepatic genes related to triglyceride synthesis and lipid accumulation. However, no significant hepatic accumulation of rifaximin was found, even after 6 months of treatment, in PXR-humanized mice. Genes in the small intestine that are involved in the uptake of fatty acids and triglycerides were induced along with increased triglyceride accumulation in intestinal epithelial cells of PXR-humanized mice; this was not observed in wild-type and Pxr-null mice. These findings suggest that long-term administration of rifaximin could lead to PXR-dependent hepatocellular fatty degeneration as a result of activation of genes involved in lipid uptake, thus indicating a potential adverse effect of rifaximin on liver function after long-term exposure.

  2. Pregnane X receptor activation induces FGF19-dependent tumor aggressiveness in humans and mice.

    Science.gov (United States)

    Wang, Hongwei; Venkatesh, Madhukumar; Li, Hao; Goetz, Regina; Mukherjee, Subhajit; Biswas, Arunima; Zhu, Liang; Kaubisch, Andreas; Wang, Lei; Pullman, James; Whitney, Kathleen; Kuro-o, Makoto; Roig, Andres I; Shay, Jerry W; Mohammadi, Moosa; Mani, Sridhar

    2011-08-01

    The nuclear receptor pregnane X receptor (PXR) is activated by a range of xenochemicals, including chemotherapeutic drugs, and has been suggested to play a role in the development of tumor cell resistance to anticancer drugs. PXR also has been implicated as a regulator of the growth and apoptosis of colon tumors. Here, we have used a xenograft model of colon cancer to define a molecular mechanism that might underlie PXR-driven colon tumor growth and malignancy. Activation of PXR was found to be sufficient to enhance the neoplastic characteristics, including cell growth, invasion, and metastasis, of both human colon tumor cell lines and primary human colon cancer tissue xenografted into immunodeficient mice. Furthermore, we were able to show that this PXR-mediated phenotype required FGF19 signaling. PXR bound to the FGF19 promoter in both human colon tumor cells and "normal" intestinal crypt cells. However, while both cell types proliferated in response to PXR ligands, the FGF19 promoter was activated by PXR only in cancer cells. Taken together, these data indicate that colon cancer growth in the presence of a specific PXR ligand results from tumor-specific induction of FGF19. These observations may lead to improved therapeutic regimens for colon carcinomas.

  3. A call center primer.

    Science.gov (United States)

    Durr, W

    1998-01-01

    Call centers are strategically and tactically important to many industries, including the healthcare industry. Call centers play a key role in acquiring and retaining customers. The ability to deliver high-quality and timely customer service without much expense is the basis for the proliferation and expansion of call centers. Call centers are unique blends of people and technology, where performance indicates combining appropriate technology tools with sound management practices built on key operational data. While the technology is fascinating, the people working in call centers and the skill of the management team ultimately make a difference to their companies.

  4. Effects and mechanisms of 3α,5α,-THP on emotion, motivation, and reward functions involving pregnane xenobiotic receptor

    Directory of Open Access Journals (Sweden)

    Cheryl A Frye

    2012-01-01

    Full Text Available Progestogens [progesterone (P4 and its products] play fundamental roles in the development and/or function of the central nervous system during pregnancy. We, and others, have investigated the role of pregnane neurosteroids for a plethora of functional effects beyond their pro-gestational processes. Emerging findings regarding the effects, mechanisms, and sources of neurosteroids have challenged traditional dogma about steroid action. How the P4 metabolite and neurosteroid, 3α-hydroxy-5α-pregnan-20-one (3α,5α-THP, influences cellular functions and behavioral processes involved in emotion/affect, motivation, and reward, is the focus of the present review. To further understand these processes, we have utilized an animal model assessing the effects, mechanisms, and sources of 3α,5α-THP. In the ventral tegmental area (VTA, 3α,5α-THP has actions to facilitate affective, and motivated, social behaviors through non-traditional targets, such as GABA, glutamate, and dopamine receptors. 3α,5α-THP levels in the midbrain VTA both facilitate, and/or are enhanced by, affective and social behavior. The pregnane xenobiotic receptor (PXR mediates the production of, and/or metabolism to, various neurobiological factors. PXR is localized to the midbrain VTA of rats. The role of PXR to influence 3α,5α-THP production from central biosynthesis, and/or metabolism of peripheral P4, in the VTA, as well as its role to facilitate, or be increased by, affective/social behaviors is under investigation. Investigating novel behavioral functions of 3α,5α-THP extends our knowledge of the neurobiology of progestogens, relevant for affective/social behaviors, and their connections to systems that regulate affect and motivated processes, such as those important for stress regulation and neuropsychiatric disorders (anxiety, depression, schizophrenia, drug dependence. Thus, further understanding of 3α,5α-THP’s role and mechanisms to enhance affective and motivated

  5. Effects and Mechanisms of 3α,5α,-THP on Emotion, Motivation, and Reward Functions Involving Pregnane Xenobiotic Receptor.

    Science.gov (United States)

    Frye, Cheryl A; Paris, J J; Walf, A A; Rusconi, J C

    2011-01-01

    Progestogens [progesterone (P(4)) and its products] play fundamental roles in the development and/or function of the central nervous system during pregnancy. We, and others, have investigated the role of pregnane neurosteroids for a plethora of functional effects beyond their pro-gestational processes. Emerging findings regarding the effects, mechanisms, and sources of neurosteroids have challenged traditional dogma about steroid action. How the P(4) metabolite and neurosteroid, 3α-hydroxy-5α-pregnan-20-one (3α,5α-THP), influences cellular functions and behavioral processes involved in emotion/affect, motivation, and reward, is the focus of the present review. To further understand these processes, we have utilized an animal model assessing the effects, mechanisms, and sources of 3α,5α-THP. In the ventral tegmental area (VTA), 3α,5α-THP has actions to facilitate affective, and motivated, social behaviors through non-traditional targets, such as GABA, glutamate, and dopamine receptors. 3α,5α-THP levels in the midbrain VTA both facilitate, and/or are enhanced by, affective and social behavior. The pregnane xenobiotic receptor (PXR) mediates the production of, and/or metabolism to, various neurobiological factors. PXR is localized to the midbrain VTA of rats. The role of PXR to influence 3α,5α-THP production from central biosynthesis, and/or metabolism of peripheral P(4), in the VTA, as well as its role to facilitate, or be increased by, affective/social behaviors is under investigation. Investigating novel behavioral functions of 3α,5α-THP extends our knowledge of the neurobiology of progestogens, relevant for affective/social behaviors, and their connections to systems that regulate affect and motivated processes, such as those important for stress regulation and neuropsychiatric disorders (anxiety, depression, schizophrenia, drug dependence). Thus, further understanding of 3α,5α-THP's role and mechanisms to enhance affective and motivated processes

  6. The Mycoestrogen Zearalenone Induces CYP3A through Activation of the Pregnane X Receptor

    Science.gov (United States)

    Ding, Xunshan; Lichti, Kristin; Staudinger, Jeff L.

    2010-01-01

    Zearalenone is a mycoestrogen that is produced in the fungi Fusarium graminearum, Fusarium culmorum, Fusarium equiseti, and Fusarium crookwellense. These fungi commonly exist in agricultural products. Human pregnane X receptor (hPXR) is a ligand-activated transcription factor that regulates the expression of numerous hepatic drug-metabolizing enzymes, including several clinically important cytochrome P450s. In this report, we show that zearalenone is an efficacious ligand for hPXR. We also describe the creation and validation of a novel adenoviral-mediated transduction protocol used to express functional FLAG-tagged-hPXR protein in a transformed cell line (HepG2) and primary cell types (cultured hepatocytes). Treatment of hPXR-transduced HepG2 cells with zearalenone induces expression of CYP3A4, the “prototypical” PXR-target gene in human liver. Treatment of hPXR-transduced cultured hepatocytes isolated from PXR-knockout mice with zearalenone induces the expression of Cyp3a11, the prototypical murine hepatic PXR-target gene. Using mammalian two-hybrid assays, we show that zearalenone displaces the nuclear receptor corepressor protein N-CoR from hPXR, while it recruits coactivator proteins steroid receptor coactivator-1, Glucocorticoid Receptor-Interacting Protein 1 and PPAR-Binding protein (GRIP1) and PBP to hPXR. Concentration-response analysis using a PXR-responsive reporter gene assay reveals that zearalenone activates hPXR with an EC50 value of approximately 1.5 μM. Because activation of hPXR represents the molecular basis of an important class of drug interactions, our findings suggest that studies to investigate the potential of zearalenone to induce the metabolism of other drugs in humans are warranted. In addition, due to the limited availability of primary human hepatocytes, our adenoviral-mediated hPXR expression protocol will likely prove useful in studies of the xenobiotic response. PMID:16547076

  7. Pregnane X receptor activation and silencing promote steatosis of human hepatic cells by distinct lipogenic mechanisms.

    Science.gov (United States)

    Bitter, Andreas; Rümmele, Petra; Klein, Kathrin; Kandel, Benjamin A; Rieger, Jessica K; Nüssler, Andreas K; Zanger, Ulrich M; Trauner, Michael; Schwab, Matthias; Burk, Oliver

    2015-11-01

    In addition to its well-characterized role in the regulation of drug metabolism and transport by xenobiotics, pregnane X receptor (PXR) critically impacts on lipid homeostasis. In mice, both ligand-dependent activation and knockout of PXR were previously shown to promote hepatic steatosis. To elucidate the respective pathways in human liver, we generated clones of human hepatoma HepG2 cells exhibiting different PXR protein levels, and analyzed effects of PXR activation and knockdown on steatosis and expression of lipogenic genes. Ligand-dependent activation as well as knockdown of PXR resulted in increased steatosis in HepG2 cells. Activation of PXR induced the sterol regulatory element-binding protein (SREBP) 1-dependent lipogenic pathway via PXR-dependent induction of SREBP1a, which was confirmed in primary human hepatocytes. Inhibiting SREBP1 activity by blocking the cleavage-dependent maturation of SREBP1 protein impaired the induction of lipogenic SREBP1 target genes and triglyceride accumulation by PXR activation. On the other hand, PXR knockdown resulted in up-regulation of aldo-keto reductase (AKR) 1B10, which enhanced the acetyl-CoA carboxylase (ACC)-catalyzed reaction step of de novo lipogenesis. In a cohort of human liver samples histologically classified for non-alcoholic fatty liver disease, AKR1B10, SREBP1a and SREBP1 lipogenic target genes proved to be up-regulated in steatohepatitis, while PXR protein was reduced. In summary, our data suggest that activation and knockdown of PXR in human hepatic cells promote de novo lipogenesis and steatosis by induction of the SREBP1 pathway and AKR1B10-mediated increase of ACC activity, respectively, thus providing mechanistic explanations for a putative dual role of PXR in the pathogenesis of steatohepatitis.

  8. Therapeutic role of rifaximin in inflammatory bowel disease: clinical implication of human pregnane X receptor activation.

    Science.gov (United States)

    Cheng, Jie; Shah, Yatrik M; Ma, Xiaochao; Pang, Xiaoyan; Tanaka, Toshiya; Kodama, Tatsuhiko; Krausz, Kristopher W; Gonzalez, Frank J

    2010-10-01

    Human pregnane X receptor (PXR) has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Rifaximin, a human PXR activator, is in clinical trials for treatment of IBD and has demonstrated efficacy in Crohn's disease and active ulcerative colitis. In the current study, the protective and therapeutic role of rifaximin in IBD and its respective mechanism were investigated. PXR-humanized (hPXR), wild-type, and Pxr-null mice were treated with rifaximin in the dextran sulfate sodium (DSS)-induced and trinitrobenzene sulfonic acid (TNBS)-induced IBD models to determine the protective function of human PXR activation in IBD. The therapeutic role of rifaximin was further evaluated in DSS-treated hPXR and Pxr-null mice. Results demonstrated that preadministration of rifaximin ameliorated the clinical hallmarks of colitis in DSS- and TNBS-treated hPXR mice as determined by body weight loss and assessment of diarrhea, rectal bleeding, colon length, and histology. In addition, higher survival rates and recovery from colitis symptoms were observed in hPXR mice, but not in Pxr-null mice, when rifaximin was administered after the onset of symptoms. Nuclear factor κB (NF-κB) target genes were markedly down-regulated in hPXR mice by rifaximin treatment. In vitro NF-κB reporter assays demonstrated inhibition of NF-κB activity after rifaximin treatment in colon-derived cell lines expressing hPXR. These findings demonstrated the preventive and therapeutic role of rifaximin on IBD through human PXR-mediated inhibition of the NF-κB signaling cascade, thus suggesting that human PXR may be an effective target for the treatment of IBD.

  9. Rifaximin is a gut-specific human pregnane X receptor activator.

    Science.gov (United States)

    Ma, Xiaochao; Shah, Yatrik M; Guo, Grace L; Wang, Ting; Krausz, Kristopher W; Idle, Jeffrey R; Gonzalez, Frank J

    2007-07-01

    Rifaximin, a rifamycin analog approved for the treatment of travelers' diarrhea, is also beneficial in the treatment of multiple chronic gastrointestinal disorders. However, the mechanisms contributing to the effects of rifaximin on chronic gastrointestinal disorders are not fully understood. In the current study, rifaximin was investigated for its role in activation of the pregnane X receptor (PXR), a nuclear receptor that regulates genes involved in xenobiotic and limited endobiotic deposition and detoxication. PXR-humanized (hPXR), Pxr-null, and wild-type mice were treated orally with rifaximin, and rifampicin, a well characterized human PXR ligand. Rifaximin was highly concentrated in the intestinal tract compared with rifampicin. Rifaximin treatment resulted in significant induction of PXR target genes in the intestine of hPXR mice, but not in wild-type and Pxr-null mice. However, rifaximin treatment demonstrated no significant effect on hepatic PXR target genes in wild-type, Pxr-null, and hPXR mice. Consistent with the in vivo data, cell-based reporter gene assay revealed rifaximin-mediated activation of human PXR, but not the other xenobiotic nuclear receptors constitutive androstane receptor, peroxisome proliferator-activated receptor (PPAR)alpha, PPARgamma, and farnesoid X receptor. Pretreatment with rifaximin did not affect the pharmacokinetics of the CYP3A substrate midazolam, but it increased the C(max) and decreased T(max) of 1'-hydroxymidazolam. Collectively, the current study identified rifaximin as a gut-specific human PXR ligand, and it provided further evidence for the utility of hPXR mice as a critical tool for the study of human PXR activators. Further human studies are suggested to assess the potential role of rifaximin-mediated gut PXR activation in therapeutics of chronic gastrointestinal disorders.

  10. Pregnane X receptor protects HepG2 cells from BaP-induced DNA damage.

    Science.gov (United States)

    Naspinski, Christine; Gu, Xinsheng; Zhou, Guo-Dong; Mertens-Talcott, Susanne U; Donnelly, Kirby C; Tian, Yanan

    2008-07-01

    Pregnane X receptor (PXR) is a nuclear receptor that coordinately regulates transcriptional expression of both phase I and phase II metabolizing enzymes. PXR plays an important role in the pharmacokinetics of a broad spectrum of endogenous and xenobiotic compounds and appears to have evolved in part to protect organisms from toxic xenobiotics. Metabolism of benzo[a]pyrene (BaP), a well-established carcinogen and ubiquitous environmental contaminant, can result in either detoxification or bioactivation to its genotoxic forms. Therefore, PXR could modulate the genotoxicity of BaP by changing the balance of the metabolic pathways in favor of BaP detoxification. To examine the role of PXR in BaP genotoxicity, BaP-DNA adduct formation was measured by 32P-postlabeling in BaP-treated parental HepG2 cells and human PXR-transfected HepG2 cells. The presence of transfected PXR significantly reduced the level of adducts relative to parental cells by 50-65% (p BaP. To analyze potential PXR-regulated detoxification pathways in liver cells, a panel of genes involved in phase I and phase II metabolism and excretion was surveyed with real-time quantitative reverse transcription PCR. The messenger RNA levels of CYP1A2, GSTA1, GSTA2, GSTM1, UGT1A6, and BCRP (ABCG2) were significantly higher in cells overexpressing PXR, independent of exposure to BaP. In addition, the total GST enzymatic activity, which favors the metabolic detoxification of BaP, was significantly increased by the presence of PXR (p BaP exposure. Taken together, these results suggest that PXR plays an important role in protection against DNA damage by polycyclic aromatic hydrocarbons (PAHs) such as BaP, and that these protective effects may be through a coordinated regulation of genes involved in xenobiotic metabolism.

  11. Purification of full-length human Pregnane and Xenobiotic Receptor: polyclonal antibody preparation for immunological characterization

    Institute of Scientific and Technical Information of China (English)

    Mallampati SARADHI; Biji KRISHNA; Gauranga MUKHOPADHYAY; Rakesh K TYAGI

    2005-01-01

    Pregnane and Xenobiotic Receptor (PXR; or Steroid and Xenobiotic Receptor, SXR), a new member of the nuclear receptor superfamily, is thought to modulate a network of genes that are involved in xenobiotic metabolism and elimination. To further explore the role of PXR in body's homeostatic mechanisms, we for the first time, report successful prokaryotic expression and purification of full-length PXR and preparation of polyclonal antibody against the whole protein. Thefull-length cDNA encoding a 434 amino acids protein was sub-cloned into prokaryotic expression vector, pET-30b and transformed into E. coli BL21 (DE3) cells for efficient over expression. The inclusion body fraction, containing the expressed recombinant protein, was purified first by solubilizing in sarcosine extraction buffer and then by affinity column chromatography using Ni-NTA His-Bind matrix. The efficacy of anti-PXR antibody was confirmed by immunocytology, Western blot analysis, EMSA and immunohistochemistry. The antibody obtained was capable of detecting human and mouse PXR with high specificity and sensitivity. Immunofluorescence staining of COS-1 cells transfected with human or mouse PXR showed a clear nuclear localization. Results from immunohistochemistry showed that level of PXR in liver sections is immunologically detectable in the nuclei. Similar to exogenously transfected PXR, Western blot analysis of cell extract from HepG2 and COLO320DM cells revealed a major protein band for endogenous PXR having the expected molecular weight of 50 kDa. Relevance of other immunodetectable bands with reference to PXR isoforms and current testimony are evaluated. Advantages of antibody raised against full-length PXR protein for functional characterization of receptor is discussed and its application for clinical purposes is envisaged.

  12. Evaluation of call options

    Institute of Scientific and Technical Information of China (English)

    陈道平

    2002-01-01

    The European and American call options,for which the prices of their underlying asset follow compound Poisson process,are evaluated by a probability method.Formulas that can be used to evaluate the options are obtained,which include not only the elements of an option:the price of the call option,the exercise price and the expiration date,but also the riskless interest rate,nevertheless exclude the volatility of the underlying asset.In practice,the evaluated results obtained by these formulas can proved references of making strategic decision for an investor who buys the call option and a company who sells the call option.

  13. Callings and Organizational Behavior

    Science.gov (United States)

    Elangovan, A. R.; Pinder, Craig C.; McLean, Murdith

    2010-01-01

    Current literature on careers, social identity and meaning in work tends to understate the multiplicity, historical significance, and nuances of the concept of calling(s). In this article, we trace the evolution of the concept from its religious roots into secular realms and develop a typology of interpretations using occupation and religious…

  14. Dimensioning large call centers

    NARCIS (Netherlands)

    S.C. Borst (Sem); A. Mandelbaum; M.I. Reiman

    2000-01-01

    textabstractWe develop a framework for asymptotic optimization of a queueing system. The motivation is the staffing problem of call centers with 100's of agents (or more). Such a call center is modeled as an M/M/N queue, where the number of agents~$N$ is large. Within our framework, we determine the

  15. Callings and Organizational Behavior

    Science.gov (United States)

    Elangovan, A. R.; Pinder, Craig C.; McLean, Murdith

    2010-01-01

    Current literature on careers, social identity and meaning in work tends to understate the multiplicity, historical significance, and nuances of the concept of calling(s). In this article, we trace the evolution of the concept from its religious roots into secular realms and develop a typology of interpretations using occupation and religious…

  16. Making telephone calls

    Institute of Scientific and Technical Information of China (English)

    任民

    2007-01-01

    @@ 1.常见用语 a.请找某人接电话 Hello!May/Could/Can I speak to…,please? b.问对方是不是某人 Is that…(speaking)? c.问对方是谁 1)Who's speaking(calling)? 2)Who's that(speaking/calling)?

  17. Synthesis of novel pregnane-diosgenin prodrugs via Ring A and Ring A connection: A combined experimental and theoretical studies

    Science.gov (United States)

    Sethi, Arun; Singh, Ranvijay Pratap; Shukla, Dolly; Singh, Praveer

    2016-12-01

    Novel pregnane-diosgenin prodrugs have been synthesized. The route involved preparation of 3β-25R-spirost-5ene 3yl-benzoate-2-carboxylic acid (2) by the esterification of diosgenin (1) with phthalic anhydride. The pregnane-diosgenin prodrugs 5 &6 were synthesized by treating 3β-25R-spirost-5ene 3yl-benzoate-2-carboxylic acid (2) with 3β-hydroxy16α-methoxy pregn-5-ene-20-one (3) and 3β-hydroxypregn-5, 16-diene-20-one (4) respectively. The synthesized compounds have been characterized with the help of spectroscopic techniques like 1H, 13C NMR, FT-IR, UV-visible spectroscopy and mass spectrometry. Density functional theory (DFT) with B3LYP functional and 6-31G (d, p) basis set has been used for the Quantum chemical calculations. UV-Vis spectra of the synthesized compounds were also recorded and electronic properties such as frontier orbitals and band gap energies were calculated by TD-DFT approach. Intramolecular interactions have been identified by AIM approach and vibrational wavenumbers have been calculated using DFT method. The reactivity and reactive site within the synthesized prodrugs were examined with reactivity descriptors (global and local). Dipole moment, polarizability and first static hyperpolarizability were calculated to get a better insight of the properties of synthesized prodrugs. The probable reaction paths of prodrugs were calculated with molecular electrostatic potential (MEP) surface analysis.

  18. QSAR model for human pregnane X receptor (PXR) binding: Screening of environmental chemicals and correlations with genotoxicity, endocrine disruption and teratogenicity

    DEFF Research Database (Denmark)

    Dybdahl, Marianne; Nikolov, Nikolai G.; Wedebye, Eva Bay

    2012-01-01

    The pregnane X receptor (PXR) has a key role in regulating the metabolism and transport of structurally diverse endogenous and exogenous compounds. Activation of PXR has the potential to initiate adverse effects, causing drug–drug interactions, and perturbing normal physiological functions...

  19. Sulforaphane is not an effective antagonist of the human pregnane X-receptor in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Poulton, Emma Jane [Center for Ecogenetics and Environmental Health, University of Washington (United States); Department of Environmental and Occupational Health Sciences, University of Washington (United States); Levy, Lisa [Public Health Sciences Division, Fred Hutchinson Cancer Research Center (United States); Lampe, Johanna W. [Center for Ecogenetics and Environmental Health, University of Washington (United States); Public Health Sciences Division, Fred Hutchinson Cancer Research Center (United States); Shen, Danny D. [Center for Ecogenetics and Environmental Health, University of Washington (United States); Department of Pharmaceutics, University of Washington (United States); Tracy, Julia [Center for Ecogenetics and Environmental Health, University of Washington (United States); Department of Environmental and Occupational Health Sciences, University of Washington (United States); Shuhart, Margaret C. [Division of Gastroenterology, Department of Medicine (United States); Thummel, Kenneth E. [Center for Ecogenetics and Environmental Health, University of Washington (United States); Department of Pharmaceutics, University of Washington (United States); Eaton, David L., E-mail: deaton@uw.edu [Center for Ecogenetics and Environmental Health, University of Washington (United States); Department of Environmental and Occupational Health Sciences, University of Washington (United States); Department of Pharmaceutics, University of Washington (United States)

    2013-01-01

    Sulforaphane (SFN), is an effective in vitro antagonist of ligand activation of the human pregnane and xenobiotic receptor (PXR). PXR mediated CYP3A4 up-regulation is implicated in adverse drug-drug interactions making identification of small molecule antagonists a desirable therapeutic goal. SFN is not an antagonist to mouse or rat PXR in vitro; thus, normal rodent species are not suitable as in vivo models for human response. To evaluate whether SFN can effectively antagonize ligand activation of human PXR in vivo, a three-armed, randomized, crossover trial was conducted with 24 healthy adults. The potent PXR ligand — rifampicin (300 mg/d) was given alone for 7 days in arm 1, or in daily combination with 450 μmol SFN (Broccoli Sprout extract) in arm 2; SFN was given alone in arm 3. Midazolam as an in vivo phenotype marker of CYP3A was administered before and after each treatment arm. Rifampicin alone decreased midazolam AUC by 70%, indicative of the expected increase in CYP3A4 activity. Co-treatment with SFN did not reduce CYP3A4 induction. Treatment with SFN alone also did not affect CYP3A4 activity in the cohort as a whole, although in the subset with the highest basal CYP3A4 activity there was a statistically significant increase in midazolam AUC (i.e., decrease in CYP3A4 activity). A parallel study in humanized PXR mice yielded similar results. The parallel effects of SFN between humanized PXR mice and human subjects demonstrate the predictive value of humanized mouse models in situations where species differences in ligand-receptor interactions preclude the use of a native mouse model for studying human ligand-receptor pharmacology. -- Highlights: ► The effects of SFN on PXR mediated CYP3A4 induction in humanized PXR mice and humans were examined. ► SFN had no effect on rifampicin mediated CYP3A4 induction in humans or humanized mice. ► SFN had a modest effect on basal CYP3A4 activity among subjects with higher baseline activity. ► Humanized PXR

  20. Involvement of promoter methylation in the regulation of Pregnane X receptor in colon cancer cells

    Directory of Open Access Journals (Sweden)

    Otsuka Koki

    2011-02-01

    Full Text Available Abstract Background Pregnane X receptor (PXR is a key transcription factor that regulates drug metabolizing enzymes such as cytochrome P450 (CYP 3A4, and plays important roles in intestinal first-pass metabolism. Although there is a large inter-individual heterogeneity with intestinal CYP3A4 expression and activity, the mechanism driving these differences is not sufficiently explained by genetic variability of PXR or CYP3A4. We examined whether epigenetic mechanisms are involved in the regulation of PXR/CYP3A4 pathways in colon cancer cells. Methods mRNA levels of PXR, CYP3A4 and vitamin D receptor (VDR were evaluated by quantitative real-time PCR on 6 colon cancer cell lines (Caco-2, HT29, HCT116, SW48, LS180, and LoVo. DNA methylation status was also examined by bisulfite sequencing of the 6 cell lines and 18 colorectal cancer tissue samples. DNA methylation was reversed by the treatment of these cell lines with 5-aza-2'-deoxycytidine (5-aza-dC. Results The 6 colon cancer cell lines were classified into two groups (high or low expression cells based on the basal level of PXR/CYP3A4 mRNA. DNA methylation of the CpG-rich sequence of the PXR promoter was more densely detected in the low expression cells (Caco-2, HT29, HCT116, and SW48 than in the high expression cells (LS180 and LoVo. This methylation was reversed by treatment with 5-aza-dC, in association with re-expression of PXR and CYP3A4 mRNA, but not VDR mRNA. Therefore, PXR transcription was silenced by promoter methylation in the low expression cells, which most likely led to downregulation of CYP3A4 transactivation. Moreover, a lower level of PXR promoter methylation was observed in colorectal cancer tissues compared with adjacent normal mucosa, suggesting upregulation of the PXR/CYP3A4 mRNAs during carcinogenesis. Conclusions PXR promoter methylation is involved in the regulation of intestinal PXR and CYP3A4 mRNA expression and might be associated with the inter-individual variability

  1. A Telephone Call

    Institute of Scientific and Technical Information of China (English)

    王晓兰

    2004-01-01

    At about two o'clock on a cold winter morning, a doctor drove seven miles in answer to a telephone call. On his coming the man who had called him in said, “Doctor, I don't have a sore throat, I don't cough.I have no pain either in the side or in the back, I'm not running a temperature, but still I feel that death is near.”

  2. Enhanced nurse call systems.

    Science.gov (United States)

    2001-04-01

    This Evaluation focuses on high-end computerized nurse call systems--what we call enhanced systems. These are highly flexible systems that incorporate microprocessor and communications technologies to expand the capabilities of the nurse call function. Enhanced systems, which vary in configuration from one installation to the next, typically consist of a basic system that provides standard nurse call functionality and a combination of additional enhancements that provide the added functionality the facility desires. In this study, we examine the features that distinguish enhanced nurse call systems from nonenhanced systems, focusing on their application and benefit to healthcare facilities. We evaluated seven systems to determine how well they help (1) improve patient care, as well as increase satisfaction with the care provided, and (2) improve caregiver efficiency, as well as increase satisfaction with the work environment. We found that all systems meet these objectives, but not all systems perform equally well for all implementations. Our ratings will help facilities identify those systems that offer the most effective features for their intended use. The study also includes a Technology Management Guide to help readers (1) determine whether they'll benefit from the capabilities offered by enhanced systems and (2) target a system for purchase and equip the system for optimum performance and cost-effective operation.

  3. Assessing call centers’ success:

    Directory of Open Access Journals (Sweden)

    Hesham A. Baraka

    2013-07-01

    This paper introduces a model to evaluate the performance of call centers based on the Delone and McLean Information Systems success model. A number of indicators are identified to track the call center’s performance. Mapping of the proposed indicators to the six dimensions of the D&M model is presented. A Weighted Call Center Performance Index is proposed to assess the call center performance; the index is used to analyze the effect of the identified indicators. Policy-Weighted approach was used to assume the weights with an analysis of different weights for each dimension. The analysis of the different weights cases gave priority to the User satisfaction and net Benefits dimension as the two outcomes from the system. For the input dimensions, higher priority was given to the system quality and the service quality dimension. Call centers decision makers can use the tool to tune the different weights in order to reach the objectives set by the organization. Multiple linear regression analysis was used in order to provide a linear formula for the User Satisfaction dimension and the Net Benefits dimension in order to be able to forecast the values for these two dimensions as function of the other dimensions

  4. Progesterone is actively metabolized to 5α-pregnane-3,20-dione and 3β-hydroxy-5α-pregnan-20-one by the marine mussel Mytilus galloprovincialis.

    Science.gov (United States)

    Dimastrogiovanni, Giorgio; Fernandes, Denise; Bonastre, Marta; Porte, Cinta

    2015-08-01

    Progesterone (P4) and synthetic progestins enter the aquatic environment through wastewater treatment plant effluents and agricultural run-off, posing potential risks to aquatic organisms due to their biological activity. P4 is a precursor of a number of steroids in vertebrates, including estrogens and androgens. Mussels Mytilus galloprovincialis were exposed to P4 at the ng to low μg/L range (0.02-10μg/L) for 7 days with the aim of (a) assessing potential alterations on endogenous steroids as a consequence of exposure, and (b) describing the enzymatic pathways involved in P4 metabolism in mussels. No significant alteration of the levels of testosterone (T) and estradiol (E2) was observed in mantle/gonad tissue of exposed mussels, in spite of a 5.6-fold increase in immunoreactive T in those exposed to 10μg P4/L, which was attributed to cross-reactivity. P4 was actively metabolized to 5α-pregnane-3,20-dione (5α-DHP) and 3β-hydroxy-5α-pregnan-20-one (3β,20-one) in digestive gland, with no evidence for the synthesis of 17α-hydroxyprogesterone or androstenedione. The metabolism of P4 to 5α-DHP was not altered by exposure. Histological examination of the gonads suggested that exposure to 10μg/L P4 induced gamete maturation and release in mussels. Nonetheless, environmental concentrations of P4 are unlikely to have an endocrine action in mussels.

  5. Call Center Capacity Planning

    DEFF Research Database (Denmark)

    Nielsen, Thomas Bang

    in modern call centers allows for a high level of customization, but also induces complicated operational processes. The size of the industry together with the complex and labor intensive nature of large call centers motivates the research carried out to understand the underlying processes. The customizable...... groups are further analyzed. The design of the overflow policies is optimized using Markov Decision Processes and a gain with regard to service levels is obtained. Also, the fixed threshold policy is investigated and found to be appropriate when one class is given high priority and when it is desired...

  6. Seco-pregnane steroidal glycosides from the roots of Cynanchum atratum and their anti-TMV activity.

    Science.gov (United States)

    Yan, Ying; Zhang, Jian-xin; Liu, Kai-xing; Huang, Tao; Yan, Chen; Huang, Lie-jun; Liu, Sheng; Mu, Shu-zhen; Hao, Xiao-jiang

    2014-09-01

    Fifteen new seco-pregnane steroidal glycosides cynanosides A-O (1-15) together with twenty-seven known ones were isolated from the roots of Cynanchum atratum. The structures of 1-15 were determined by extensive analysis of spectroscopic data. The anti-tobacco mosaic virus (TMV) activity of these steroidal glycosides was screened by the conventional half-leaf method, enzyme-linked immunosorbent assay, and Western blot methods, most of them showed potent anti-TMV activity. Among them, compounds 1, 7, 13, 28 and 31 showed significantly anti-TMV activity with an IC50 value of 20.5, 18.6, 22.0, 19.2 and 22.2 μg/mL, respectively, and were much more effective than the positive control, ningnanmycin (IC50=49.6 μg/mL). Copyright © 2014 Elsevier B.V. All rights reserved.

  7. CALLING AQUARIUM LOVERS...

    CERN Multimedia

    2002-01-01

    CERN's anemones will soon be orphans. We are looking for someone willing to look after the aquarium in the main building, for one year. If you are interested, or if you would like more information, please call 73830. (The anemones living in the aquarium thank you in anticipation.)

  8. A call for surveys

    DEFF Research Database (Denmark)

    Bernstein, Philip A.; Jensen, Christian S.; Tan, Kian-Lee

    2012-01-01

    The database field is experiencing an increasing need for survey papers. We call on more researchers to set aside time for this important writing activity. The database field is growing in population, scope of topics covered, and the number of papers published. Each year, thousands of new papers...

  9. A call for surveys

    DEFF Research Database (Denmark)

    Bernstein, Philip A.; Jensen, Christian S.; Tan, Kian-Lee

    2012-01-01

    The database field is experiencing an increasing need for survey papers. We call on more researchers to set aside time for this important writing activity. The database field is growing in population, scope of topics covered, and the number of papers published. Each year, thousands of new papers ...

  10. Novel receptor targets for production and action of allopregnanolone in the central nervous system: a focus on pregnane xenobiotic receptor

    Directory of Open Access Journals (Sweden)

    Cheryl A Frye

    2014-04-01

    Full Text Available Neurosteroids are cholesterol-based hormones that can be produced in the brain, independent of secretion from peripheral endocrine glands, such as the gonads and adrenals. A focus in our laboratory for over 25 years has been how production of the pregnane neurosteroid, allopregnanolone, is regulated and the novel (i.e. non steroid receptor targets for steroid action for behavior. One endpoint of interest has been lordosis, the mating posture of female rodents. Allopregnanolone is necessary and sufficient for lordosis, and the brain circuitry underlying it, such as actions in the midbrain ventral tegmental area (VTA, has been well-characterized. Published and recent findings supporting a dynamic role of allopregnanolone are included in this review. First, contributions of ovarian and adrenal sources of precursors of allopregnanolone, and the requisite enzymatic actions for de novo production in the central nervous system will be discussed. Second, how allopregnanolone produced in the brain has actions on behavioral processes that are independent of binding to steroid receptors, but instead involve rapid modulatory actions via neurotransmitter targets (e.g. -amino butyric acid-GABA, n-methyl-D-aspartate- NMDA will be reviewed. Third, a recent focus on characterizing the role of a promiscuous nuclear receptor, pregnane xenobiotic receptor (PXR, involved in cholesterol metabolism and expressed in the VTA, as a target for allopregnanolone and how this relates to both actions and production of allopregnanolone will be addressed. For example, allopregnanolone can bind PXR and knocking down expression of PXR in the midbrain VTA attenuates actions of allopregnanolone via NMDA and/or GABAA for lordosis. Our understanding of allopregnanolone’s actions in the VTA for lordosis has been extended to reveal the role of allopregnanolone for broader, clinically-relevant questions, such as neuropsychiatric disorders, epilepsy, and aging.

  11. Synthesis and biological activity of two pregnane derivatives with a triazole or imidazole ring at C-21.

    Science.gov (United States)

    Silva-Ortiz, Aylin Viviana; Bratoeff, Eugene; Ramírez-Apan, María Teresa; García-Becerra, Rocío; Ordaz-Rosado, David; Noyola-Martínez, Nancy; Castillo-Bocanegra, Rafael; Barrera, David

    2016-05-01

    Pregnane derivatives are studied as agents for the treatment of different hormone-dependent diseases. The biological importance of these steroids is based on their potential use against cancer. In this study, we report the synthesis, characterization and biological activity of two pregnane derivatives with a triazole (3β-hydroxy-21-(1H-1,2,4-triazol-1-yl)pregna-5,16-dien-20-one; T-OH) or imidazole (3β-hydroxy-21-(1H-imidazol-1-yl)pregna-5,16-dien-20-one; I-OH) moieties at C-21. These derivatives were synthesized from 16-dehydropregnenolone acetate. The activity on cell proliferation of the compounds was measured on three human cancer cells lines: prostate cancer (PC-3), breast cancer (MCF7) and lung cancer (SK-LU-1). The cytotoxic and antiproliferative effects of T-OH and I-OH were assessed by using SBR and XTT methods, respectively. The gene expressions were evaluated by real time PCR. In addition, results were complemented by docking studies and transactivation assays using an expression vector to progesterone and androgen receptor. Results show that the two compounds inhibited the three cell lines proliferation in a dose-dependent manner. Compound I-OH downregulated the gene expression of the cyclins D1 and E1 in PC-3 and MFC7 cells; however, effect upon Ki-67, EAG1, BIM or survivin genes was not observed. Docking studies show poor interaction with the steroid receptors. Nevertheless, the transactivation assays show a weak antagonist effect of I-OH on progesterone receptor but not androgenic or antiandrogenic actions. In conclusion, the synthesized compounds inhibited cell proliferation as well as genes key to cell cycle of PC-3 and MCF7 cell lines. Therefore, these compounds could be considered a good starting point for the development of novel therapeutic alternatives to treat cancer.

  12. Flight calls and orientation

    DEFF Research Database (Denmark)

    Larsen, Ole Næsbye; Andersen, Bent Bach; Kropp, Wibke

    2008-01-01

    flight calls was simulated by sequential computer controlled activation of five loudspeakers placed in a linear array perpendicular to the bird's migration course. The bird responded to this stimulation by changing its migratory course in the direction of that of the ‘flying conspecifics' but after about......  In a pilot experiment a European Robin, Erithacus rubecula, expressing migratory restlessness with a stable orientation, was video filmed in the dark with an infrared camera and its directional migratory activity was recorded. The flight overhead of migrating conspecifics uttering nocturnal...... 30 minutes it drifted back to its original migration course. The results suggest that songbirds migrating alone at night can use the flight calls from conspecifics as additional cues for orientation and that they may compare this information with other cues to decide what course to keep....

  13. Calling Dunbar's Numbers

    CERN Document Server

    MacCarron, Pádraig; Dunbar, Robin

    2016-01-01

    The social brain hypothesis predicts that humans have an average of about 150 relationships at any given time. Within this 150, there are layers of friends of an ego, where the number of friends in a layer increases as the emotional closeness decreases. Here we analyse a mobile phone dataset, firstly, to ascertain whether layers of friends can be identified based on call frequency. We then apply different clustering algorithms to break the call frequency of egos into clusters and compare the number of alters in each cluster with the layer size predicted by the social brain hypothesis. In this dataset we find strong evidence for the existence of a layered structure. The clustering yields results that match well with previous studies for the innermost and outermost layers, but for layers in between we observe large variability.

  14. Flight calls and orientation

    DEFF Research Database (Denmark)

    Larsen, Ole Næsbye; Andersen, Bent Bach; Kropp, Wibke

    2008-01-01

      In a pilot experiment a European Robin, Erithacus rubecula, expressing migratory restlessness with a stable orientation, was video filmed in the dark with an infrared camera and its directional migratory activity was recorded. The flight overhead of migrating conspecifics uttering nocturnal...... flight calls was simulated by sequential computer controlled activation of five loudspeakers placed in a linear array perpendicular to the bird's migration course. The bird responded to this stimulation by changing its migratory course in the direction of that of the ‘flying conspecifics' but after about...... 30 minutes it drifted back to its original migration course. The results suggest that songbirds migrating alone at night can use the flight calls from conspecifics as additional cues for orientation and that they may compare this information with other cues to decide what course to keep....

  15. Call centres: constructing flexibility

    OpenAIRE

    Arzbächer, Sandra; Holtgrewe, Ursula; Kerst, Christian

    2002-01-01

    "The development of call centres as a flexible interface between firms and their environments has been seen as exemplary or even symptomatic of flexible capitalism (Sennett 1998). We are going to point out that they do not just stand for organisational change but also for changes of institutions towards deregulation. Employers and managers hoped for gains of flexibility, decreasing labour costs, and market gains by an expanded 24-hour-service. Surveillance and control by flexib...

  16. How Dolphins Call

    Institute of Scientific and Technical Information of China (English)

    马加芬

    2007-01-01

    <正>与人类一样,海豚能通过声音相互称呼"名字"。A high-pitched"wee-o-wee-o-wee-o-wee"whistle might not soundlike much to you,but it6s exactly how a dolphin might introduce itself.Because sight is limited in the ocean,dolphins create individual"name"calls to communicate their whereabouts to friends and families.But it6snot as simple as just recognizing a voice,

  17. Heed the Calls

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    @@ THE bombing of Libya by some of the world's major powers, including France, the United States and Britain brought a new dimension to the protests that have washed over North Africa and the Middle East in the past months.Following the UN Security Council passing the imposition of a no-fly zone over Libyan air-space, a move supported by Nigeria, South Africa and Gabon, air and sea strikes pounded Muammar Gadhafi's strongholds.The AU called for an immediate stop to hostilities on both sides.

  18. Heed the Calls

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    THE bombing of Libya by some of the world’s major powers,including France, the United States and Britain brought a new dimension to the protests that have washed over North Africa and the Middle East in the past months.Following the UN Security Council passing the imposition of a no-fly zone over Libyan air-space,a move supported by Nigeria,South Africa and Gabon,air and sea strikes pounded Muammar Gadhafi’s strongholds.The AU called for an immediate stop to hostilities

  19. Emergency Call Reform

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    China merges several hotline call services in an effort to streamline the urban emergency system Chances are that if you find yourself in a crisis situation in China, dialing the correct emergency number could be a problem. Not because there isn't one, but quite the opposite. China has four main emergency response numbers-110 summons police help in a life-threatening situation, 119 brings out firefighters, 122 gets police activated to sort out traffic accidents and 120 ensures medical help. Along with th...

  20. An Island Called Cuba

    Directory of Open Access Journals (Sweden)

    Jean Stubbs

    2011-06-01

    Full Text Available Review of: An Island Called Home: Returning to Jewish Cuba. Ruth Behar, photographs by Humberto Mayol. New Brunswick NJ: Rutgers University Press, 2007. xiii + 297 pp. (Cloth US$ 29.95 Fidel Castro: My Life: A Spoken Autobiography. Fidel Castro & Ignacio Ramonet. New York: Scribner/Simon & Schuster, 2008. vii + 724 pp. (Paper US$ 22.00, e-book US$ 14.99 Cuba: What Everyone Needs to Know. Julia E. Sweig. New York: Oxford University Press, 2009. xiv + 279 pp. (Paper US$ 16.95 [First paragraph] These three ostensibly very different books tell a compelling story of each author’s approach, as much as the subject matter itself. Fidel Castro: My Life: A Spoken Autobiography is based on a series of long interviews granted by the then-president of Cuba, Fidel Castro, to Spanish-Franco journalist Ignacio Ramonet. Cuba: What Everyone Needs to Know, by U.S. political analyst Julia Sweig, is one of a set country series, and, like Ramonet’s, presented in question/answer format. An Island Called Home: Returning to Jewish Cuba, with a narrative by Cuban-American anthropologist Ruth Behar and photographs by Cuban photographer Humberto Mayol, is a retrospective/introspective account of the Jewish presence in Cuba. While from Ramonet and Sweig we learn much about the revolutionary project, Behar and Mayol convey the lived experience of the small Jewish community against that backdrop.

  1. Too close to call

    DEFF Research Database (Denmark)

    Kurrild-Klitgaard, Peter

    2012-01-01

    The note briefly outlines a new model for the explanation of US presidential elections, founded on (a) recent economic growth and (b) a measure of what may be called “’the cost of ruling”. The former is based in changes in real disposable income for the period following a mid-term election, while...... the latter combines factors of incumbency and terms-in-office. The model is applied to data from the US presidential elections 1932-2008 and has considerable explanatory power for the variation in the incumbent party’s candidate’s share of the two-party vote (R2=0.74). The model is controlled against...... a number of other frequent explanations and is found to be quite robust. When augmented with approval ratings for incumbent presidents, the explanatory power increases to 83 pct. and only incorrectly calls one of the last 15 US presidential elections. Applied to the 2012 election as a forecasting model...

  2. MEDICAL SERVICE - URGENT CALLS

    CERN Multimedia

    Service Médical

    2000-01-01

    IN URGENT NEED OF A DOCTOR GENEVA: EMERGENCY SERVICES GENEVA AND VAUD 144 FIRE BRIGADE 118 POLICE 117 CERN FIREMEN 767-44-44 ANTI-POISONS CENTRE Open 24h/24h 01-251-51-51 Patient not fit to be moved, call family doctor, or: GP AT HOME: Open 24h/24h 748-49-50 AMG- Association Of Geneva Doctors: Emergency Doctors at home 07h-23h 322 20 20 Patient fit to be moved: HOPITAL CANTONAL CENTRAL 24 Micheli-du-Crest 372-33-11 ou 382-33-11 EMERGENCIES 382-33-11 ou 372-33-11 CHILDREN'S HOSPITAL 6 rue Willy-Donzé 372-33-11 MATERNITY 32 bvd.de la Cluse 382-68-16 ou 382-33-11 OPHTHALMOLOGY 22 Alcide Jentzer 382-33-11 ou 372-33-11 MEDICAL CENTRE CORNAVIN 1-3 rue du Jura 345 45 50 HOPITAL DE LA TOUR Meyrin 719-61-11 EMERGENCIES 719-61-11 CHILDREN'S EMERGENCIES 719-61-00 LA TOUR MEDICAL CENTRE 719-74-00 European Emergency Call 112   FRANCE: EMERGENCY SERVICES 15 FIRE BRIGADE 18 POLICE 17 CERN FIREMEN AT HOME 00-41-22-767-44-44 ...

  3. Call for volunteers

    CERN Multimedia

    2008-01-01

    CERN is calling for volunteers from all members of the Laboratory for organizing the two exceptional Open days.CERN is calling for volunteers from all members of the Laboratory’s personnel to help with the organisation of these two exceptional Open Days, for the visits of CERN personnel and their families on the Saturday and above all for the major public Open Day on the Sunday. As for the 50th anniversary in 2004, the success of the Open Days will depend on a large number of volunteers. All those working for CERN as well as retired members of the personnel can contribute to making this event a success. Many guides will be needed at the LHC points, for the activities at the surface and to man the reception and information points. The aim of these major Open Days is to give the local populations the opportunity to discover the fruits of almost 20 years of work carried out at CERN. We are hoping for some 2000 volunteers for the two Open Days, on the Saturday from 9 a.m. to ...

  4. Too close to call

    DEFF Research Database (Denmark)

    Kurrild-Klitgaard, Peter

    2012-01-01

    The note briefly outlines a new model for the explanation of US presidential elections, founded on (a) recent economic growth and (b) a measure of what may be called “’the cost of ruling”. The former is based in changes in real disposable income for the period following a mid-term election, while...... the latter combines factors of incumbency and terms-in-office. The model is applied to data from the US presidential elections 1932-2008 and has considerable explanatory power for the variation in the incumbent party’s candidate’s share of the two-party vote (R2=0.74). The model is controlled against...

  5. Roles of rifampicin in drug-drug interactions: underlying molecular mechanisms involving the nuclear pregnane X receptor

    Directory of Open Access Journals (Sweden)

    Raymond Kenneth

    2006-02-01

    Full Text Available Abstract Rifampicin, an important drug in the treatment of tuberculosis, is used extensively despite its broad effects on drug-drug interactions, creating serious problems. The clinical importance of such interactions includes autoinduction leading to suboptimal or failed treatment. The concomitantly administered effects of rifampicin on other drugs can result in their altered metabolism or transportation that are metabolised by cytochromes P450 or transported by p-glycoprotein in the gastrointestinal tract and liver. This review paper summarises recent findings with emphases on the molecular mechanisms used to explain these broad drug-drug interactions. In general, rifampicin can act on a pattern: rifampicin activates the nuclear pregnane X receptor that in turn affects cytochromes P450, glucuronosyltransferases and p-glycoprotein activities. This pattern of action may explain many of the rifampicin inducing drug-drug interactions. However, effects through other mechanisms have also been reported and these make any explanation of such drug-drug interactions more complex.

  6. Sesamin: A Naturally Occurring Lignan Inhibits CYP3A4 by Antagonizing the Pregnane X Receptor Activation.

    Science.gov (United States)

    Lim, Yun-Ping; Ma, Chia-Yun; Liu, Cheng-Ling; Lin, Yu-Hsien; Hu, Miao-Lin; Chen, Jih-Jung; Hung, Dong-Zong; Hsieh, Wen-Tsong; Huang, Jin-Ding

    2012-01-01

    Inconsistent expression and regulation of drug-metabolizing enzymes (DMEs) are common causes of adverse drug effects in some drugs with a narrow therapeutic index (TI). An important cytochrome, cytochrome P450 3A4 (CYP3A4), is predominantly regulated by a nuclear receptor, pregnane X receptor (PXR). Sesamin, a major lignan constituent in sesame seeds and oil, exhibits a variety of biological functions; however, the effect of sesamin on the modulation of CYP3A4 is not well understood. In this study, the effects of sesamin on the PXR-CYP3A4 pathway were characterized, as well as the underlying mechanisms of those effects. Sesamin potently attenuated CYP3A4 induction in a dose-dependent manner by blocking the activation of PXR. The PXR inducer-mediated inhibition of CYP3A4 was further evidenced by the ability of sesamin to attenuate the effects of several PXR ligands in the CYP3A4 reporter assay. Further mechanistic studies showed that sesamin inhibited PXR by interrupting the interacting with coregulators. These results may lead to the development of new therapeutic and dietary approaches to reduce the frequency of inducer-drug interaction. Sesamin was established as a novel inhibitor of PXR and may be useful for modulating DMEs expression and drug efficacies. Modification of CYP3A4 expression and activity by consumption of sesamin may have important implications for drug safety.

  7. Sesamin: A Naturally Occurring Lignan Inhibits CYP3A4 by Antagonizing the Pregnane X Receptor Activation

    Directory of Open Access Journals (Sweden)

    Yun-Ping Lim

    2012-01-01

    Full Text Available Inconsistent expression and regulation of drug-metabolizing enzymes (DMEs are common causes of adverse drug effects in some drugs with a narrow therapeutic index (TI. An important cytochrome, cytochrome P450 3A4 (CYP3A4, is predominantly regulated by a nuclear receptor, pregnane X receptor (PXR. Sesamin, a major lignan constituent in sesame seeds and oil, exhibits a variety of biological functions; however, the effect of sesamin on the modulation of CYP3A4 is not well understood. In this study, the effects of sesamin on the PXR-CYP3A4 pathway were characterized, as well as the underlying mechanisms of those effects. Sesamin potently attenuated CYP3A4 induction in a dose-dependent manner by blocking the activation of PXR. The PXR inducer-mediated inhibition of CYP3A4 was further evidenced by the ability of sesamin to attenuate the effects of several PXR ligands in the CYP3A4 reporter assay. Further mechanistic studies showed that sesamin inhibited PXR by interrupting the interacting with coregulators. These results may lead to the development of new therapeutic and dietary approaches to reduce the frequency of inducer-drug interaction. Sesamin was established as a novel inhibitor of PXR and may be useful for modulating DMEs expression and drug efficacies. Modification of CYP3A4 expression and activity by consumption of sesamin may have important implications for drug safety.

  8. Accessing the molecular interactions of phthalates and their primary metabolites with the human pregnane X receptor using in silico profiling.

    Science.gov (United States)

    Sarath Josh, M K; Pradeep, S; Balan, Aparna K; Sreejith, M N; Benjamin, Sailas

    2016-12-01

    Phthalates are known to cause endocrine disruption in humans and animals. Being lipophilic xenobiotic chemicals, phthalates from the surrounding environments can easily be absorbed into the biological system, thereby causing various health dysfunctions. This molecular docking study evaluates a variety of molecular interactions of 12 commonly used diphthalates and respective monophthalates onto the ligand binding domain (LBD) of the human pregnane X receptor (hPXR), a xenosensor, which would be beneficial for further in vitro and in vivo studies on hazardous phthalates. Out of 12 diphthalates and their monophthalates tested, diisodecyl phthalate (-9.16 kcal mol(-1) ) showed more affinity toward hPXR whereas diisononyl phthalate (-8.77) and di(2-ethyhexyl)phthalate (-8.56), the predominant plasticizers found in a variety of plastics and allied products, showed comparable binding scores with that of the control ligands such as hyperforine (-9.99) and dexamethasone (-7.36). In addition to the above diphthalates, some of their monophthalates (monoisodecyl phthalate, mono-2-etheylhexyl phthalate, etc.) also established similar interactions with certain crucial amino acids in the LBD, which led to higher G scores. In fact, bisphenol A, a well-studied and proven endocrine disruptor, showed lesser G scores (-6.69) than certain phthalates. Copyright © 2016 John Wiley & Sons, Ltd.

  9. Activation of Intestinal Human Pregnane X Receptor Protects against Azoxymethane/Dextran Sulfate Sodium–Induced Colon Cancer

    Science.gov (United States)

    Cheng, Jie; Fang, Zhong-Ze; Nagaoka, Kenjiro; Okamoto, Minoru; Qu, Aijuan; Tanaka, Naoki; Kimura, Shioko

    2014-01-01

    The role of intestinal human pregnane X receptor (PXR) in colon cancer was determined through investigation of the chemopreventive role of rifaximin, a specific agonist of intestinal human PXR, toward azoxymethane (AOM)/dextran sulfate sodium (DSS)–induced colon cancer. Rifaximin treatment significantly decreased the number of colon tumors induced by AOM/DSS treatment in PXR-humanized mice, but not wild-type or Pxr-null mice. Additionally, rifaximin treatment markedly increased the survival rate of PXR-humanized mice, but not wild-type or Pxr-null mice. These data indicated a human PXR–dependent therapeutic chemoprevention of rifaximin toward AOM/DSS-induced colon cancer. Nuclear factor κ-light-chain-enhancer of activated B cells–mediated inflammatory signaling was upregulated in AOM/DSS-treated mice, and inhibited by rifaximin in PXR-humanized mice. Cell proliferation and apoptosis were also modulated by rifaximin treatment in the AOM/DSS model. In vitro cell-based assays further revealed that rifaximin regulated cell apoptosis and cell cycle in a human PXR-dependent manner. These results suggested that specific activation of intestinal human PXR exhibited a chemopreventive role toward AOM/DSS-induced colon cancer by mediating anti-inflammation, antiproliferation, and proapoptotic events. PMID:25277138

  10. Activation of intestinal human pregnane X receptor protects against azoxymethane/dextran sulfate sodium-induced colon cancer.

    Science.gov (United States)

    Cheng, Jie; Fang, Zhong-Ze; Nagaoka, Kenjiro; Okamoto, Minoru; Qu, Aijuan; Tanaka, Naoki; Kimura, Shioko; Gonzalez, Frank J

    2014-12-01

    The role of intestinal human pregnane X receptor (PXR) in colon cancer was determined through investigation of the chemopreventive role of rifaximin, a specific agonist of intestinal human PXR, toward azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colon cancer. Rifaximin treatment significantly decreased the number of colon tumors induced by AOM/DSS treatment in PXR-humanized mice, but not wild-type or Pxr-null mice. Additionally, rifaximin treatment markedly increased the survival rate of PXR-humanized mice, but not wild-type or Pxr-null mice. These data indicated a human PXR-dependent therapeutic chemoprevention of rifaximin toward AOM/DSS-induced colon cancer. Nuclear factor κ-light-chain-enhancer of activated B cells-mediated inflammatory signaling was upregulated in AOM/DSS-treated mice, and inhibited by rifaximin in PXR-humanized mice. Cell proliferation and apoptosis were also modulated by rifaximin treatment in the AOM/DSS model. In vitro cell-based assays further revealed that rifaximin regulated cell apoptosis and cell cycle in a human PXR-dependent manner. These results suggested that specific activation of intestinal human PXR exhibited a chemopreventive role toward AOM/DSS-induced colon cancer by mediating anti-inflammation, antiproliferation, and proapoptotic events.

  11. Oleanolic Acid-Mediated Inhibition of Pregnane X Receptor and Constitutive Androstane Receptor Attenuates Rifampin-Isoniazid Cytotoxicity.

    Science.gov (United States)

    Lin, Yen-Ning; Chen, Chao-Jung; Chang, Hsiao-Yun; Cheng, Wai-Kok; Lee, Ying-Ray; Chen, Jih-Jung; Lim, Yun-Ping

    2017-10-04

    Interactions between transcriptional inducers of cytochrome P450 (CYP450) and pharmacological agents might decrease drug efficacy and induce side effects. Such interactions could be prevented using an antagonist of the pregnane X receptor (PXR) and constitutive androstane receptor (CAR). Here, we aimed to determine the antagonistic effect of oleanolic acid (OA) on PXR and CAR. OA attenuated the promoter activities, expressions, and enzyme catalytic activities of CYP3A4 and CYP2B6 mediated by rifampin (RIF) and CITCO. Moreover, OA displayed species specificity for rodent PXR. Interaction of coregulators with PXR and transcriptional complexes on the CYP3A4 promoter was disrupted by OA. Additionally, OA reversed the cytotoxic effects of isoniazid induced by RIF. These data demonstrate that OA inhibited the transactivation of PXR and CAR, reduced the expression and function of CYP3A4 and CYP2B6, and may therefore serve as an effective agent for reducing probability adverse interactions between transcriptional inducers of CYP450 and therapeutic drugs.

  12. Call Centre- Computer Telephone Integration

    Directory of Open Access Journals (Sweden)

    Dražen Kovačević

    2012-10-01

    Full Text Available Call centre largely came into being as a result of consumerneeds converging with enabling technology- and by the companiesrecognising the revenue opportunities generated by meetingthose needs thereby increasing customer satisfaction. Regardlessof the specific application or activity of a Call centre, customersatisfaction with the interaction is critical to the revenuegenerated or protected by the Call centre. Physical(v, Call centreset up is a place that includes computer, telephone and supervisorstation. Call centre can be available 24 hours a day - whenthe customer wants to make a purchase, needs information, orsimply wishes to register a complaint.

  13. Kjernereseptorar og endokrinforstyrrande stoff: Ligandaktiveringsstudiar av human Pregnan X Reseptor (hPXR) og human Østrogen Reseptor alfa (hERalfa) med luciferase transfeksjonsassay

    OpenAIRE

    Sørland, Gunn-Therese Lund

    2008-01-01

    Samandrag Pregnan X Reseptor (PXR) er ein kjernereseptor som ved aktivering kan indusere transkripsjon av CYP3A4 enzym. Desse enzyma er involvert i metabolisme av mange sambindingar som til dømes legemiddel. PXR vert aktivert ved å binde ligand. Det er identifisert svært mange ligandar for PXR. Aktivering av PXR kan altså seiast å vere ein sentral prosess i metabolisme utført av CYP3A4 enzymet. Det er derfor viktig med auka kunnskap om kva ligandar og ligandkonsentrasjonar som kan aktiver...

  14. Thalidomide increases human hepatic cytochrome P450 3A enzymes by direct activation of the pregnane X receptor.

    Science.gov (United States)

    Murayama, Norie; van Beuningen, Rinie; Suemizu, Hiroshi; Guguen-Guillouzo, Christiane; Shibata, Norio; Yajima, Kanako; Utoh, Masahiro; Shimizu, Makiko; Chesné, Christophe; Nakamura, Masato; Guengerich, F Peter; Houtman, René; Yamazaki, Hiroshi

    2014-02-17

    Heterotropic cooperativity of human cytochrome P450 (P450) 3A4/3A5 by the teratogen thalidomide was recently demonstrated by H. Yamazaki et al. ( ( 2013 ) Chem. Res. Toxicol. 26 , 486 - 489 ) using the model substrate midazolam in various in vitro and in vivo models. Chimeric mice with humanized liver also displayed enhanced midazolam clearance upon pretreatment with orally administered thalidomide, presumably because of human P450 3A induction. In the current study, we further investigated the regulation of human hepatic drug metabolizing enzymes. Thalidomide enhanced levels of P450 3A4 and 2B6 mRNA, protein expression, and/or oxidation activity in human hepatocytes, indirectly suggesting the activation of upstream transcription factors involved in detoxication, e.g., the nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR). A key event after ligand binding is an alteration of nuclear receptor conformation and recruitment of coregulator proteins that alter chromatin accessibility of target genes. To investigate direct engagement and functional alteration of PXR and CAR by thalidomide, we utilized a peptide microarray with 154 coregulator-derived nuclear receptor-interaction motifs and coregulator and nuclear receptor boxes, which serves as a sensor for nuclear receptor conformation and activity status as a function of ligand. Thalidomide and its human proximate metabolite 5-hydroxythalidomide displayed significant modulation of coregulator interaction with PXR and CAR ligand-binding domains, similar to established agonists for these receptors. These results collectively suggest that thalidomide acts as a ligand for PXR and CAR and causes enzyme induction leading to increased P450 enzyme activity. The possibilities of drug interactions during thalidomide therapy in humans require further evaluation.

  15. Environmental contaminants activate human and polar bear (Ursus maritimus) pregnane X receptors (PXR, NR1I2) differently

    Energy Technology Data Exchange (ETDEWEB)

    Lille-Langøy, Roger, E-mail: Roger.lille-langoy@bio.uib.no [University of Bergen, Department of Biology, P.O. Box 7803, N-5020 Bergen (Norway); Goldstone, Jared V. [Woods Hole Oceanographic Institution, 266 Woods Hole Road, 02543-1050 Woods Hole, MA (United States); Rusten, Marte [University of Bergen, Department of Molecular Biology, P.O. Box 7803, N-5020 Bergen (Norway); Milnes, Matthew R. [Mars Hill University, 100 Athletic Street, Box 6671, Mars Hill, 28754 NC (United States); Male, Rune [University of Bergen, Department of Molecular Biology, P.O. Box 7803, N-5020 Bergen (Norway); Stegeman, John J. [Woods Hole Oceanographic Institution, 266 Woods Hole Road, 02543-1050 Woods Hole, MA (United States); Blumberg, Bruce [University of California, Irvine, 92697 CA (United States); Goksøyr, Anders [University of Bergen, Department of Biology, P.O. Box 7803, N-5020 Bergen (Norway)

    2015-04-01

    Background: Many persistent organic pollutants (POPs) accumulate readily in polar bears because of their position as apex predators in Arctic food webs. The pregnane X receptor (PXR, formally NR1I2, here proposed to be named promiscuous xenobiotic receptor) is a xenobiotic sensor that is directly involved in metabolizing pathways of a wide range of environmental contaminants. Objectives: In the present study, we comparably assess the ability of 51 selected pharmaceuticals, pesticides and emerging contaminants to activate PXRs from polar bears and humans using an in vitro luciferase reporter gene assay. Results: We found that polar bear PXR is activated by a wide range of our test compounds (68%) but has a slightly more narrow ligand specificity than human PXR that was activated by 86% of the 51 test compounds. The majority of the agonists identified (70%) produces a stronger induction of the reporter gene via human PXR than via polar bear PXR, however with some notable and environmentally relevant exceptions. Conclusions: Due to the observed differences in activation of polar bear and human PXRs, exposure of each species to environmental agents is likely to induce biotransformation differently in the two species. Bioinformatics analyses and structural modeling studies suggest that amino acids that are not part of the ligand-binding domain and do not interact with the ligand can modulate receptor activation. - Highlights: • Comparative study of ligand activation of human and polar bear PXRs. • Polar bear PXR is a promiscuous ligand-activated nuclear receptor but less so than human PXR. • Environmental contaminants activate human and polar bear PXRs differently. • Expression and ligand promiscuity indicate that PXR is a xenosensor in polar bears.

  16. Phenobarbital induces cell cycle transcriptional responses in mouse liver humanized for constitutive androstane and pregnane x receptors.

    Science.gov (United States)

    Luisier, Raphaëlle; Lempiäinen, Harri; Scherbichler, Nina; Braeuning, Albert; Geissler, Miriam; Dubost, Valerie; Müller, Arne; Scheer, Nico; Chibout, Salah-Dine; Hara, Hisanori; Picard, Frank; Theil, Diethilde; Couttet, Philippe; Vitobello, Antonio; Grenet, Olivier; Grasl-Kraupp, Bettina; Ellinger-Ziegelbauer, Heidrun; Thomson, John P; Meehan, Richard R; Elcombe, Clifford R; Henderson, Colin J; Wolf, C Roland; Schwarz, Michael; Moulin, Pierre; Terranova, Rémi; Moggs, Jonathan G

    2014-06-01

    The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms underlying liver tissue molecular responses to PB. Early and late transcriptomic responses to sustained PB exposure were investigated in liver tissue from double knock-out CAR and PXR (CAR(KO)-PXR(KO)), double humanized CAR and PXR (CAR(h)-PXR(h)), and wild-type C57BL/6 mice. Wild-type and CAR(h)-PXR(h) mouse livers exhibited temporally and quantitatively similar transcriptional responses during 91 days of PB exposure including the sustained induction of the xenobiotic response gene Cyp2b10, the Wnt signaling inhibitor Wisp1, and noncoding RNA biomarkers from the Dlk1-Dio3 locus. Transient induction of DNA replication (Hells, Mcm6, and Esco2) and mitotic genes (Ccnb2, Cdc20, and Cdk1) and the proliferation-related nuclear antigen Mki67 were observed with peak expression occurring between 1 and 7 days PB exposure. All these transcriptional responses were absent in CAR(KO)-PXR(KO) mouse livers and largely reversible in wild-type and CAR(h)-PXR(h) mouse livers following 91 days of PB exposure and a subsequent 4-week recovery period. Furthermore, PB-mediated upregulation of the noncoding RNA Meg3, which has recently been associated with cellular pluripotency, exhibited a similar dose response and perivenous hepatocyte-specific localization in both wild-type and CAR(h)-PXR(h) mice. Thus, mouse livers coexpressing human CAR and PXR support both the xenobiotic metabolizing and the proliferative transcriptional responses following exposure to PB.

  17. Pregnane-X-receptor mediates the anti-inflammatory activities of rifaximin on detoxification pathways in intestinal epithelial cells.

    Science.gov (United States)

    Mencarelli, Andrea; Migliorati, Marco; Barbanti, Miriam; Cipriani, Sabrina; Palladino, Giuseppe; Distrutti, Eleonora; Renga, Barbara; Fiorucci, Stefano

    2010-12-01

    The pregnane-X-receptor (PXR) is master gene overseeing detoxification of wide number of xenobiotics and is critical for maintenance of intestinal integrity. The intestinal expression of genes involved in cellular detoxification is down-regulated in patients with inflammatory bowel diseases (IBD). Rifaximin is a non-absorbable antibiotic endowed with a PXR agonistic activity. In the present study we have investigated whether rifaximin activates PXR in primary human colon epithelial cells and human colon biopsies and assessed whether this antibiotic antagonizes the effect of tumor necrosis factor (TNF)-α on expression of PXR and PXR-related genes. Present results demonstrate that primary colon epithelial cells express PXR and that their exposure to rifaximin induces the expression of genes involved in cellular detoxification. Exposure to TNFα reduces the expression of PXR mRNA as well as expression of its target genes. This inhibitory effect was prevented by that co-treatment with rifaximin. Knocking down the expression of PXR in colon epithelial cells by an anti-PXR siRNA, abrogated the counter-regulatory effects exerted by rifaximin on cell exposed to TNFα. Finally, ex vivo exposure of colon biopsies obtained from ulcerative colitis patients to rifaximin increased the expression of genes involved in xenobiotics metabolism. In aggregate, these data illustrate that rifaximin increases the expression of PXR and PXR-regulated genes involved in the metabolism and excretion of xenobiotics and antagonizes the effects of TNFα in intestinal epithelial cells and colon biopsies. These non-antibiotic effects of rifaximin could contribute to the maintenance of the intestinal barrier integrity against xenobiotics and products generated by luminal bacteria.

  18. Comparative study of human and mouse pregnane X receptor agonistic activity in 200 pesticides using in vitro reporter gene assays.

    Science.gov (United States)

    Kojima, Hiroyuki; Sata, Fumihiro; Takeuchi, Shinji; Sueyoshi, Tatsuya; Nagai, Tadanori

    2011-02-27

    The nuclear receptor, pregnane X receptor (PXR), is a ligand-dependent transcription factor that regulates genes involved in xenobiotic metabolism. Recent studies have shown that PXR activation may affect energy metabolism as well as the endocrine and immune systems. In this study, we characterized and compared the agonistic activities of a variety of pesticides against human PXR (hPXR) and mouse PXR (mPXR). We tested the hPXR and mPXR agonistic activity of 200 pesticides (29 organochlorines, 11 diphenyl ethers, 56 organophosphorus pesticides, 12 pyrethroids, 22 carbamates, 12 acid amides, 7 triazines, 7 ureas, and 44 others) by reporter gene assays using COS-7 simian kidney cells. Of the 200 pesticides tested, 106 and 93 activated hPXR and mPXR, respectively, and a total of 111 had hPXR and/or mPXR agonistic activity with greater or lesser inter-species differences. Although all of the pyrethroids and most of the organochlorines and acid amides acted as PXR agonists, a wide range of pesticides with diverse structures also showed hPXR and/or mPXR agonistic activity. Among the 200 pesticides, pyributicarb, pretilachlor, piperophos and butamifos for hPXR, and phosalone, prochloraz, pendimethalin, and butamifos for mPXR, acted as particularly potent activators at low concentrations in the order of 10⁻⁸-10⁻⁷ M. In addition, we found that several organophosphorus oxon- and pyributicarb oxon-metabolites decreased PXR activation potency compared to their parent compounds. These results suggest that a large number of structurally diverse pesticides and their metabolites possess PXR-mediated transcriptional activity, and their ability to do so varies in a species-dependent manner in humans and mice. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

  19. Rifampicin-activated human pregnane X receptor and CYP3A4 induction enhance acetaminophen-induced toxicity.

    Science.gov (United States)

    Cheng, Jie; Ma, Xiaochao; Krausz, Kristopher W; Idle, Jeffrey R; Gonzalez, Frank J

    2009-08-01

    Acetaminophen (APAP) is safe at therapeutic levels but causes hepatotoxicity via N-acetyl-p-benzoquinone imine-induced oxidative stress upon overdose. To determine the effect of human (h) pregnane X receptor (PXR) activation and CYP3A4 induction on APAP-induced hepatotoxicity, mice humanized for PXR and CYP3A4 (TgCYP3A4/hPXR) were treated with APAP and rifampicin. Human PXR activation and CYP3A4 induction enhanced APAP-induced hepatotoxicity as revealed by hepatic alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities elevated in serum, and hepatic necrosis after coadministration of rifampicin and APAP, compared with APAP administration alone. In contrast, hPXR mice, wild-type mice, and Pxr-null mice exhibited significantly lower ALT/AST levels compared with TgCYP3A4/hPXR mice after APAP administration. Toxicity was coincident with depletion of hepatic glutathione and increased production of hydrogen peroxide, suggesting increased oxidative stress upon hPXR activation. Moreover, mRNA analysis demonstrated that CYP3A4 and other PXR target genes were significantly induced by rifampicin treatment. Urinary metabolomic analysis indicated that cysteine-APAP and its metabolite S-(5-acetylamino-2-hydroxyphenyl)mercaptopyruvic acid were the major contributors to the toxic phenotype. Quantification of plasma APAP metabolites indicated that the APAP dimer formed coincident with increased oxidative stress. In addition, serum metabolomics revealed reduction of lysophosphatidylcholine in the APAP-treated groups. These findings demonstrated that human PXR is involved in regulation of APAP-induced toxicity through CYP3A4-mediated hepatic metabolism of APAP in the presence of PXR ligands.

  20. A novel statistical algorithm for gene expression analysis helps differentiate pregnane X receptor-dependent and independent mechanisms of toxicity.

    Directory of Open Access Journals (Sweden)

    M Ann Mongan

    Full Text Available Genome-wide gene expression profiling has become standard for assessing potential liabilities as well as for elucidating mechanisms of toxicity of drug candidates under development. Analysis of microarray data is often challenging due to the lack of a statistical model that is amenable to biological variation in a small number of samples. Here we present a novel non-parametric algorithm that requires minimal assumptions about the data distribution. Our method for determining differential expression consists of two steps: 1 We apply a nominal threshold on fold change and platform p-value to designate whether a gene is differentially expressed in each treated and control sample relative to the averaged control pool, and 2 We compared the number of samples satisfying criteria in step 1 between the treated and control groups to estimate the statistical significance based on a null distribution established by sample permutations. The method captures group effect without being too sensitive to anomalies as it allows tolerance for potential non-responders in the treatment group and outliers in the control group. Performance and results of this method were compared with the Significant Analysis of Microarrays (SAM method. These two methods were applied to investigate hepatic transcriptional responses of wild-type (PXR(+/+ and pregnane X receptor-knockout (PXR(-/- mice after 96 h exposure to CMP013, an inhibitor of β-secretase (β-site of amyloid precursor protein cleaving enzyme 1 or BACE1. Our results showed that CMP013 led to transcriptional changes in hallmark PXR-regulated genes and induced a cascade of gene expression changes that explained the hepatomegaly observed only in PXR(+/+ animals. Comparison of concordant expression changes between PXR(+/+ and PXR(-/- mice also suggested a PXR-independent association between CMP013 and perturbations to cellular stress, lipid metabolism, and biliary transport.

  1. Application of Affect to CALL

    Institute of Scientific and Technical Information of China (English)

    李露

    2006-01-01

    This essay mainly attempts to discuss some affective factors in CALL, with focus on affective influence on English (foreign language) learning and thus concludes that in the process of CALL related affect should be activated and made good use of to better foreign language teaching and learning.

  2. The difficult medical emergency call

    DEFF Research Database (Denmark)

    Møller, Thea Palsgaard; Kjærulff, Thora Majlund; Viereck, Søren

    2017-01-01

    BACKGROUND: Pre-hospital emergency care requires proper categorization of emergency calls and assessment of emergency priority levels by the medical dispatchers. We investigated predictors for emergency call categorization as "unclear problem" in contrast to "symptom-specific" categories and the ......BACKGROUND: Pre-hospital emergency care requires proper categorization of emergency calls and assessment of emergency priority levels by the medical dispatchers. We investigated predictors for emergency call categorization as "unclear problem" in contrast to "symptom-specific" categories...... and the effect of categorization on mortality. METHODS: Register-based study in a 2-year period based on emergency call data from the emergency medical dispatch center in Copenhagen combined with nationwide register data. Logistic regression analysis (N = 78,040 individuals) was used for identification...

  3. The difficult medical emergency call

    DEFF Research Database (Denmark)

    Møller, Thea Palsgaard; Kjærulff, Thora Majlund; Viereck, Søren

    2017-01-01

    BACKGROUND: Pre-hospital emergency care requires proper categorization of emergency calls and assessment of emergency priority levels by the medical dispatchers. We investigated predictors for emergency call categorization as "unclear problem" in contrast to "symptom-specific" categories and the ......BACKGROUND: Pre-hospital emergency care requires proper categorization of emergency calls and assessment of emergency priority levels by the medical dispatchers. We investigated predictors for emergency call categorization as "unclear problem" in contrast to "symptom-specific" categories...... and the effect of categorization on mortality. METHODS: Register-based study in a 2-year period based on emergency call data from the emergency medical dispatch center in Copenhagen combined with nationwide register data. Logistic regression analysis (N = 78,040 individuals) was used for identification...

  4. Involvement of Nuclear Factor κB, not Pregnane X Receptor, in Inflammation-Mediated Regulation of Hepatic Transporters.

    Science.gov (United States)

    Abualsunun, Walaa A; Piquette-Miller, Micheline

    2017-10-01

    Endotoxin-induced inflammation decreases the hepatic expression of several drug transporters, metabolizing enzymes, and nuclear transcription factors, including pregnane X receptor (PXR). As the nuclear factor κB (NF-κB) is a major mediator of inflammation, and reciprocal repression between NF-κB and PXR signaling has been reported, the objective of this study was to examine whether NF-κB directly regulates the expression of transporters or exerts its effect indirectly via PXR. PXR-deficient (-/-) or wild-type (+/+) male mice were dosed with the selective NF-κB inhibitor PHA408 (40 mg/kg i.p.) or vehicle (n = 5-8/group), followed by endotoxin (5 mg/kg) or saline 30 minutes later. Animals were sacrificed at 6 hours; samples were analyzed using quantitative reverse-transcription polymerase chain reaction and Western blots. Endotoxin induced tumor necrosis factor-α, interleukin (IL)-6, IL-1β, and inducible nitric oxide synthase in PXR (+/+) and (-/-) mice. As compared with saline controls, endotoxin administration imposed 30%-70% significant decreases in the expression of Abcb1a, Abcb11, Abcc2, Abcc3, Abcg2, Slc10a1, Slco2b1, and Slco1a4 in PXR (+/+) and (-/-) mice to a similar extent. Preadministration of PHA408 attenuated endotoxin-mediated changes in both PXR (+/+) and (-/-) mice (P < 0.05). Our findings demonstrate that endotoxin activates NF-κB and imposes a downregulation of numerous ATP-binding cassette and solute carrier transporters through NF-κB in liver and is independent of PXR. Moreover, inhibition of NF-κB attenuates the impact of endotoxin on transporter expression. As NF-κB activation is involved in many acute and chronic disease states, disease-induced changes in transporter function may be an important source of variability in drug response. This information may be useful in predicting potential drug-disease interactions. Copyright © 2017 by The American Society for Pharmacology and Experimental Therapeutics.

  5. Overexpression of pregnane X and glucocorticoid receptors and the regulation of cytochrome P450 in human epileptic brain endothelial cells.

    Science.gov (United States)

    Ghosh, Chaitali; Hossain, Mohammed; Solanki, Jesal; Najm, Imad M; Marchi, Nicola; Janigro, Damir

    2017-04-01

    Recent evidence suggests a metabolic contribution of cytochrome P450 enzymes (CYPs) to the drug-resistant phenotype in human epilepsy. However, the upstream molecular regulators of CYP in the epileptic brain remain understudied. We therefore investigated the expression and function of pregnane xenobiotic (PXR) and glucocorticoid (GR) nuclear receptors in endothelial cells established from post-epilepsy surgery brain samples. PXR/GR localization was evaluated by immunohistochemistry in specimens from subjects who underwent temporal lobe resections to relieve drug-resistant seizures. We used primary cultures of endothelial cells obtained from epileptic brain tissues (EPI-ECs; n = 8), commercially available human brain microvascular endothelial cells (HBMECs; n = 8), and human hepatocytes (n = 3). PXR/GR messenger RNA (mRNA) levels in brain ECs was initially determined by complementary DNA (cDNA) microarrays. The expression of PXR/GR proteins was quantified by Western blot. PXR and GR silencing was performed in EPI-ECs (n = 4), and the impact on downstream CYP expression was determined. PXR/GR expression was detected by immunofluorescence in ECs and neurons in the human temporal lobe samples analyzed. Elevated mRNA and protein levels of PXR and GR were found in EPI-ECs versus control HBMECs. Hepatocytes, used as a positive control, displayed the highest levels of PXR/GR expression. We confirmed expression of PXR/GR in cytoplasmic-nuclear subcellular fractions, with a significant increase of PXR/GR in EPI-ECs versus controls. CYP3A4, CYP2C9, and CYP2E1 were overexpressed in EPI-ECs versus control, whereas CYP2D6 and CYP2C19 were downregulated or absent in EPI-ECs. GR silencing in EPI-ECs led to decreased CYP3A4, CYP2C9, and PXR expression. PXR silencing in EPI-ECs resulted in the specific downregulation of CYP3A4 expression. Our results indicate increased PXR and GR in primary ECs derived from human epileptic brains. PXR or GR may be responsible for a local drug brain

  6. Threonine-408 Regulates the Stability of Human Pregnane X Receptor through Its Phosphorylation and the CHIP/Chaperone-Autophagy Pathway.

    Science.gov (United States)

    Sugatani, Junko; Noguchi, Yuji; Hattori, Yoshiki; Yamaguchi, Masahiko; Yamazaki, Yasuhiro; Ikari, Akira

    2016-01-01

    The human pregnane X receptor (hPXR) is a xenobiotic-sensing nuclear receptor that transcriptionally regulates drug metabolism-related genes. The aim of the present study was to elucidate the mechanism by which hPXR is regulated through threonine-408. A phosphomimetic mutation at threonine-408 (T408D) reduced the transcriptional activity of hPXR and its protein stability in HepG2 and SW480 cells in vitro and mouse livers in vivo. Proteasome inhibitors (calpain inhibitor I and MG132) and Hsp90 inhibitor geldanamycin, but not Hsp70 inhibitor pifithrin-μ, increased wild-type (WT) hPXR in the nucleus. The translocation of the T408D mutant to the nucleus was significantly reduced even in the presence of proteasome inhibitors, whereas the complex of yellow fluorescent protein (YFP)-hPXR T408D mutant with heat shock cognate protein 70/heat shock protein 70 and carboxy terminus Hsp70-interacting protein (CHIP; E3 ligase) was similar to that of the WT in the cytoplasm. Treatment with pifithrin-μ and transfection with anti-CHIP small-interfering RNA reduced the levels of CYP3A4 mRNA induced by rifampicin, suggesting the contribution of Hsp70 and CHIP to the transactivation of hPXR. Autophagy inhibitor 3-methyladenine accumulated YFP-hPXR T408D mutant more efficiently than the WT in the presence of proteasome inhibitor lactacystin, and the T408D mutant colocalized with the autophagy markers, microtubule-associated protein 1 light chain 3 and p62, which were contained in the autophagic cargo. Lysosomal inhibitor chloroquine caused the marked accumulation of the T408D mutant in the cytoplasm. Protein kinase C (PKC) directly phosphorylated the threonine-408 of hPXR. These results suggest that hPXR is regulated through its phosphorylation at threonine-408 by PKC, CHIP/chaperone-dependent stability check, and autophagic degradation pathway. Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics.

  7. The Call of Humanity in The Call of the Wild

    Institute of Scientific and Technical Information of China (English)

    朱露; 张磊

    2011-01-01

    Jack London is one of the greatest American novelists. Many of his works are very popular among readers all over the world. This paper firstly gives a brief introduction of Jack London and The Call of the Wild. Then it analyzes the theme and main Characters of this novel. At last the implied meaning is discussed.

  8. Detection of pregnancy and fertility status in big cats using an enzyme immunoassay based on 5α-pregnan-3α-ol-20-one.

    Science.gov (United States)

    Umapathy, Govindhaswamy; Kumar, Vinod; Wasimuddin; Kabra, Meha; Shivaji, S

    2013-01-01

    Development of non-invasive steroid hormone assays using fecal samples is crucial for detection of pregnancy and monitoring of fertility status in big cats and thus facilitates conservation and management of wild animals. Due to changes in metabolism and excretory pattern, animals excrete different steroid metabolites in feces and urine. The present study is an attempt to develop a common enzyme immunoassay for 5α-pregnan-3α-ol-20-one one of the predominant progestogen metabolites in the feces samples of big cats. The developed ELISA showed a high sensitivity and low cross reactivity to other hormones compared to commercially available RIA kits based on progesterone antibody. It could be used in a wide range of animals for monitoring fertility status and pregnancy detection by measuring fecal steroid metabolites.

  9. The Wireless Nursing Call System

    DEFF Research Database (Denmark)

    Jensen, Casper Bruun

    2006-01-01

    This paper discusses a research project in which social scientists were involved both as analysts and supporters during a pilot with a new wireless nursing call system. The case thus exemplifies an attempt to participate in developing dependable health care systems and offers insight into the cha......This paper discusses a research project in which social scientists were involved both as analysts and supporters during a pilot with a new wireless nursing call system. The case thus exemplifies an attempt to participate in developing dependable health care systems and offers insight...

  10. Learning as Calling and Responding

    Science.gov (United States)

    Jons, Lotta

    2014-01-01

    According to Martin Buber's philosophy of dialogue, our being-in-the-world is to be conceived of as an existential dialogue. Elsewhere, I have conceptualized the teacher-student-relation accordingly (see Jons 2008), as a matter of calling and responding. The conceptualization rests on a secularised notion of vocation, paving way for…

  11. An Evaluation Framework for CALL

    Science.gov (United States)

    McMurry, Benjamin L.; Williams, David Dwayne; Rich, Peter J.; Hartshorn, K. James

    2016-01-01

    Searching prestigious Computer-assisted Language Learning (CALL) journals for references to key publications and authors in the field of evaluation yields a short list. The "American Journal of Evaluation"--the flagship journal of the American Evaluation Association--is only cited once in both the "CALICO Journal and Language…

  12. Calle y Saberes en Movimiento

    Directory of Open Access Journals (Sweden)

    Laura Daniela Aguirre Aguilar

    2010-01-01

    Full Text Available En México el rezago, el ausentismo, la deserción escolar, el trabajo a temprana edad y el inicio de una vida en la calle, en repetidas ocasiones son consecuencia de un núcleo familiar desarticulado o de una débil relación intrafamiliar, así como de una condición socioeconómica en desventaja. Ante esta problemática, la Secretaría de Educación Pública, instancia gubernamental encargada de garantizar una educación de calidad para la población, trabaja coordinadamente con organizaciones de la sociedad civil e instancias públicas, para la reintegración a los espacios educativos de los niños, niñas y jóvenes en situación de calle.

  13. Call for improving air quality

    Science.gov (United States)

    Showstack, Randy

    2013-01-01

    The European Environmental Bureau (EEB), a federation of citizen organizations, has called for stricter policies in Europe to protect human health and the environment. "Air pollution emanates from sources all around us, be they cars, industrial plants, shipping, agriculture, or waste. The [European Union] must propose ambitious legislation to address all of these sources if it is to tackle the grave public health consequences of air pollution," EEB secretary general Jeremy Wates said on 8 January.

  14. Ultrasound call detection in capybara

    Directory of Open Access Journals (Sweden)

    Selene S.C. Nogueira

    2012-07-01

    Full Text Available The vocal repertoire of some animal species has been considered a non-invasive tool to predict distress reactivity. In rats ultrasound emissions were reported as distress indicator. Capybaras[ vocal repertoire was reported recently and seems to have ultrasound calls, but this has not yet been confirmed. Thus, in order to check if a poor state of welfare was linked to ultrasound calls in the capybara vocal repertoire, the aim of this study was to track the presence of ultrasound emissions in 11 animals under three conditions: 1 unrestrained; 2 intermediately restrained, and 3 highly restrained. The ultrasound track identified frequencies in the range of 31.8±3.5 kHz in adults and 33.2±8.5 kHz in juveniles. These ultrasound frequencies occurred only when animals were highly restrained, physically restrained or injured during handling. We concluded that these calls with ultrasound components are related to pain and restraint because they did not occur when animals were free of restraint. Thus we suggest that this vocalization may be used as an additional tool to assess capybaras[ welfare.

  15. Advanced Call Center Supporting WAP Access

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Traditional call centers can be accessed via speech only, and the call center based on web provides both data and speech access, but it needs a powerful terminal-computer. By analyzing traditional call centers and call centers based on web, this paper presents the framework of an advanced call center supporting WAP access. A typical service is also described in detail.

  16. 47 CFR 2.302 - Call signs.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Call signs. 2.302 Section 2.302... RULES AND REGULATIONS Call Signs and Other Forms of Identifying Radio Transmissions § 2.302 Call signs. The table which follows indicates the composition and blocks of international call signs available...

  17. Indirubin, a component of Ban-Lan-Gen, activates CYP3A4 gene transcription through the human pregnane X receptor.

    Science.gov (United States)

    Kumagai, Takeshi; Aratsu, Yusuke; Sugawara, Ryosuke; Sasaki, Takamitsu; Miyairi, Shinichi; Nagata, Kiyoshi

    2016-04-01

    Ban-Lan-Gen is the common name for the dried roots of indigo plants, including Polygonum tinctorium, Isatis indigotica, Isatis tinctoria, and Strobilanthes cusia. Ban-Lan-Gen is frequently used as an anti-inflammatory and an anti-viral for the treatment of hepatitis, influenza, and various types of inflammation. One of the cytochrome P450 (CYP) enzymes, CYP3A4, is responsible for the metabolism of a wide variety of xenobiotics, including an estimated 60% of all clinically used drugs. In this study, we investigated the effect of Ban-Lan-Gen on the transcriptional activation of the CYP3A4 gene. Ban-Lan-Gen extract increased CYP3A4 gene reporter activity in a dose-dependent manner. Indirubin, one of the biologically active ingredients in the Ban-Lan-Gen, also dose-dependently increased CYP3A4 gene reporter activity. Expression of short hairpin RNA for the human pregnane X receptor (hPXR-shRNA) inhibited CYP3A4 gene reporter activity, and overexpression of human PXR increased indirubin- and rifampicin-induced CYP3A4 gene reporter activity. Furthermore, indirubin induced CYP3A4 mRNA expression in HepG2 cells. Taken together, these results indicate that indirubin, a component of Ban-Lan-Gen, activated CYP3A4 gene transcription through the activation of the human PXR.

  18. Regulation of human pregnane X receptor and its target gene cytochrome P450 3A4 by Chinese herbal compounds and a molecular docking study.

    Science.gov (United States)

    Liu, Ya-He; Mo, Sui-Lin; Bi, Hui-Chang; Hu, Bing-Fang; Li, Chun Guang; Wang, Yi-Tao; Huang, Ling; Huang, Min; Duan, Wei; Liu, Jun-Ping; Wei, Ming Qian; Zhou, Shu-Feng

    2011-04-01

    The pregnane X receptor (PXR) plays a critical role in the regulation of human cytochrome P450 3A4 (CYP3A4) gene. In this study, we investigated the effect of an array of compounds isolated from Chinese herbal medicines on the activity of PXR using a luciferase reporter gene assay in transiently transfected HepG2 and Huh7 cells and on the expression of PXR and CYP3A4 in LS174T cells. Furthermore, molecular docking was performed to investigate the binding modes of herbal compounds with PXR. Praeruptorin A and C, salvianolic acid B, sodium danshensu, protocatechuic aldehyde, cryptotanshinone, emodin, morin, and tanshinone IIA significantly transactivated the CYP3A4 reporter gene construct in either HepG2 or Huh7 cells. The PXR mRNA expression in LS174T cells was significantly induced by physcion, protocatechuic aldehyde, salvianolic acid B, and sodium danshensu. However, epifriedelanol, morin, praeruptorin D, mulberroside A, tanshinone I, and tanshinone IIA significantly down-regulated the expression of PXR mRNA in LS174T cells. All the herbal compounds tested can be readily docked into the ligand-binding cavity of PXR mainly through hydrogen bond and aromatic interactions with Ser247, Gln285, His407, and Arg401. These findings suggest that herbal medicines can significantly regulate PXR and CYP3A4 and this has important implication in herb-drug interactions.

  19. Induction of drug metabolism by forskolin: the role of the pregnane X receptor and the protein kinase a signal transduction pathway.

    Science.gov (United States)

    Ding, Xunshan; Staudinger, Jeff L

    2005-02-01

    An extract of the plant Coleus forskohlii has been used for centuries in Ayurvedic medicine to treat various diseases such as hypothyroidism, heart disease, and respiratory disorders. Additionally, complex herbal mixtures containing this extract are gaining popularity in United States for their putative "fat-burning" properties. The active ingredient in C. forskohlii extract is the diterpene compound forskolin. Forskolin is a widely used biochemical tool that activates adenyl cyclase, thereby increasing intracellular concentration of cAMP and thus activating the protein kinase A (PKA) signal transduction pathway. We show herein that both forskolin and its nonadenyl cyclase-activating analog 1,9 dideoxyforskolin induce CYP3A gene expression in primary hepatocytes by functioning as agonists of the pregnane X receptor (PXR). We show that activation of PKA signaling potentiates PXR-mediated induction of CYP3A gene expression in cultured hepatocytes and increases the strength of PXR-coactivator protein-protein interaction in cell-based assays. Kinase assays show that PXR can serve as a substrate for catalytically active PKA in vitro. Our data provide important insights into the molecular mechanism of both the PKA-dependent and -independent effects of forskolin on the expression of drug-metabolizing enzymes in liver. Finally, our data suggest that herbal therapy with C. forskohlii extract should be approached cautiously due to the potential for herb-drug interactions in patients on combination therapy.

  20. Echinacea purpurea up-regulates CYP1A2, CYP3A4 and MDR1 gene expression by activation of pregnane X receptor pathway.

    Science.gov (United States)

    Awortwe, Charles; Manda, Vamshi K; Avonto, Cristina; Khan, Shabana I; Khan, Ikhlas A; Walker, Larry A; Bouic, Patrick J; Rosenkranz, Bernd

    2015-03-01

    1.This study investigated the mechanism underlying Echinacea-mediated induction of CYP1A2, CYP3A4 and MDR1 in terms of human pregnane X receptor (PXR) activation. 2.Crude extracts and fractions of Echinacea purpurea were tested for PXR activation in HepG2 cells by a reporter gene assay. Quantitative real-time PCR was carried out to determine their effects on CYP1A2 and CYP3A4 mRNA expressions. Capsules and fractions were risk ranked as high, intermediate and remote risk of drug-metabolizing enzymes induction based on EC50 values determined for respective CYPs. 3. Fractions F1, F2 and capsule (2660) strongly activated PXR with 5-, 4- and 3.5-fold increase in activity, respectively. Echinacea preparations potentiated up-regulation of CYP1A2, CYP3A4 and MDR1 via PXR activation. 4.Thus E. purpurea preparations cause herb-drug interaction by up-regulating CYP1A2, CYP3A4 and P-gp via PXR activation.

  1. Biomonitoring of non-dioxin-like polychlorinated biphenyls in transgenic Arabidopsis using the mammalian pregnane X receptor system: a role of pectin in pollutant uptake.

    Directory of Open Access Journals (Sweden)

    Lieming Bao

    Full Text Available Polychlorinated biphenyls (PCBs are persistent organic pollutants damaging to human health and the environment. Techniques to indicate PCB contamination in planta are of great interest to phytoremediation. Monitoring of dioxin-like PCBs in transgenic plants carrying the mammalian aryl hydrocarbon receptor (AHR has been reported previously. Herein, we report the biomonitoring of non-dioxin-like PCBs (NDL-PCBs using the mammalian pregnane X receptor (PXR. In the transgenic Arabidopsis designated NDL-PCB Reporter, the EGFP-GUS reporter gene was driven by a promoter containing 18 repeats of the xenobiotic response elements, while PXR and its binding partner retinoid X receptor (RXR were coexpressed. Results showed that, in live cells, the expression of reporter gene was insensitive to endogenous lignans, carotenoids and flavonoids, but responded to all tested NDL-PCBs in a dose- and time- dependent manner. Two types of putative PCB metabolites, hydroxy- PCBs and methoxy- PCBs, displayed different activation properties. The vascular tissues seemed unable to transport NDL-PCBs, whereas mutation in QUASIMODO1 encoding a 1,4-galacturonosyltransferase led to reduced PCB accumulation in Arabidopsis, revealing a role for pectin in the control of PCB translocation. Taken together, the reporter system may serve as a useful tool to biomonitor the uptake and metabolism of NDL-PCBs in plants.

  2. Pregnane X receptor (PXR-mediated gene repression and cross-talk of PXR with other nuclear receptors via coactivator interactions

    Directory of Open Access Journals (Sweden)

    Petr Pavek

    2016-11-01

    Full Text Available Pregnane X receptor is a ligand-activated nuclear receptor that mainly controls inducible expression of xenobiotics handling genes including biotransformation enzymes and drug transporters. Nowadays it is clear that PXR is also involved in regulation of intermediate metabolism through trans-activation and trans-repression of genes controlling glucose, lipid, cholesterol, bile acid and bilirubin homeostasis. In these processes PXR cross-talks with other nuclear receptors. Accumulating evidence suggests that the cross-talk is often mediated by competing for common coactivators or by disruption of coactivation and activity of other transcription factors by the ligand-activated PXR. In this respect mainly PXR-CAR and PXR-HNF4α interference have been reported and several cytochrome P450 enzymes (such as CYP7A1 and CYP8B1, phase II enzymes (SULT1E1, Gsta2, Ugt1a1, drug and endobiotic transporters (OCT1, Mrp2, Mrp3, Oatp1a and Oatp4 as well as intermediate metabolism enzymes (PEPCK1 and G6Pase have been shown as down-regulated genes after PXR activation. In this review, I summarize our current knowledge of PXR-mediated repression and coactivation interference in PXR-controlled gene expression regulation.

  3. Pregnane X Receptor (PXR)-Mediated Gene Repression and Cross-Talk of PXR with Other Nuclear Receptors via Coactivator Interactions

    Science.gov (United States)

    Pavek, Petr

    2016-01-01

    Pregnane X receptor is a ligand-activated nuclear receptor (NR) that mainly controls inducible expression of xenobiotics handling genes including biotransformation enzymes and drug transporters. Nowadays it is clear that PXR is also involved in regulation of intermediate metabolism through trans-activation and trans-repression of genes controlling glucose, lipid, cholesterol, bile acid, and bilirubin homeostasis. In these processes PXR cross-talks with other NRs. Accumulating evidence suggests that the cross-talk is often mediated by competing for common coactivators or by disruption of coactivation and activity of other transcription factors by the ligand-activated PXR. In this respect mainly PXR-CAR and PXR-HNF4α interference have been reported and several cytochrome P450 enzymes (such as CYP7A1 and CYP8B1), phase II enzymes (SULT1E1, Gsta2, Ugt1a1), drug and endobiotic transporters (OCT1, Mrp2, Mrp3, Oatp1a, and Oatp4) as well as intermediate metabolism enzymes (PEPCK1 and G6Pase) have been shown as down-regulated genes after PXR activation. In this review, I summarize our current knowledge of PXR-mediated repression and coactivation interference in PXR-controlled gene expression regulation. PMID:27932985

  4. Advanced Call Center Supporting WAP Access

    Institute of Scientific and Technical Information of China (English)

    YUANXiao-hua; CHENJun-liang

    2001-01-01

    Traditional call centers can be accessed via speech only, and the call center based on web provides both da-ta and speech access,but it needs a powerful terminal-computer.By analyzing traditional call centers and call cen-ters based on web, this paper presents the framework of an advanced call center supporting WAP access.A typical service is also described in detail.

  5. The function of migratory bird calls

    DEFF Research Database (Denmark)

    Reichl, Thomas; Andersen, Bent Bach; Larsen, Ole Næsbye

    the experimental bird could be activated successively to simulate a migrating Robin cruising E-W, W-E, S-N or N-S at a chosen height (mostly about 40 m), at 10 m/s and emitting Robin flight calls of 80 dB(A) at 1 m. The simulated flight of a "ding" sound served as a control. During an experiment the bird was first...... Denmark, Odense, DK-5230 Odense M, Denmark   Many migrating passerines emit special calls during nocturnal flight, the so-called flight calls. Several functions of the calls have been suggested but largely remain speculative. Flight calls have been hypothesized to maintain groups during nocturnal...... migration and to stimulate migratory restlessness in conspecifics. We wished to test if conspecific flight calls influence the flight direction of a nocturnal migrant, the European Robin (Erithacus rubecula), i.e. if flight calls help migrants keeping course. Wild caught birds showing migratory restlessness...

  6. 76 FR 17934 - Infrastructure Protection Data Call

    Science.gov (United States)

    2011-03-31

    ... SECURITY Infrastructure Protection Data Call AGENCY: National Protection and Programs Directorate, DHS...: Infrastructure Protection Data Call. OMB Number: 1670-NEW. Frequency: On occasion. Affected Public: Federal...: The Department of Homeland Security (DHS), National Protection and Programs Directorate (NPPD),...

  7. Steroids excreted in urine by neonates with 21-hydroxylase deficiency: characterization, using GC-MS and GC-MS/MS, of the D-ring and side chain structure of pregnanes and pregnenes.

    Science.gov (United States)

    Christakoudi, Sofia; Cowan, David A; Taylor, Norman F

    2010-01-01

    Steroid metabolites in urine from neonates with 21-hydroxylase deficiency are predominantly polyhydroxylated 17-hydroxyprogesterone and androgen metabolites, and most have incompletely defined structure. This study forms part of a comprehensive project to characterize and identify these in order to enhance diagnosis and to further elucidate neonatal types of steroid metabolism. Steroids were analyzed, after extraction and enzymatic conjugate hydrolysis, as methyloxime-trimethylsilyl ether derivatives on gas-chromatographs coupled to quadrupole and ion-trap mass-spectrometers. GC-MS and GC-MS/MS spectra, obtained with constant excitation conditions, were used together to determine the structure of the D-ring and the side chain of 20-oxo and 20-hydroxy pregnane(ene)s without oxo groups on the A-, B-, and C-ring. All possible combinations of D-ring and side chain configuration were considered. Most fragmentations could be interpreted as partial or complete D-ring cleavages with loss of the side chain, aided by comparison with spectra of deuterated derivatives and of borohydride reduced metabolites. Possible rearrangement ions are also discussed. More than 140 endogenous metabolites were characterized. GC-MS/MS was especially beneficial for characterization of compounds with 16,17-dihydroxy-20-oxo structure, interpreted as markers of intra-uterine enzyme induction. It also assisted the differentiation of 16-hydroxy-20-oxo metabolites, present in urine of non-affected neonates, from the diagnostic 17-hydroxy-20-oxosteroids and enabled the detection of 15,17-dihydroxy-20-oxo compounds in low concentrations. The presence of 17,21-dihydroxylated pregnane(ene)s despite the deficit in CYP21A2 is discussed. We conclude that GC-MS combined with GC-MS/MS allows reliable identification of the structure of the D-ring and side chain of pregnane(ene)s without prior isolation, even when in low concentrations in urine.

  8. HOW TO CALL THE CERN FIRE BRIGADE

    CERN Multimedia

    2001-01-01

    The telephone numbers of the CERN Fire Brigade are: 74444 for emergency calls 74848 for other calls Note The number 112 will stay in use for emergency calls from 'wired' telephones, however, from mobile phones it leads to non-CERN emergency services.

  9. How to call the Fire Brigade

    CERN Multimedia

    2003-01-01

    The telephone numbers for the CERN Fire Brigade are: 74444 for emergency calls 74848 for other calls Note The number 112 will stay in use for emergency calls from "wired" telephones, however, from mobile phones it leads to non-CERN emergency services.

  10. HOW TO CALL THE CERN FIRE BRIGADE

    CERN Multimedia

    2002-01-01

    The telephone numbers of the CERN Fire Brigade are: 74444 for emergency calls 74848 for other calls Note The number 112 will stay in use for emergency calls from 'wired' telephones, however, from mobile phones it leads to non-CERN emergency services.

  11. HOW TO CALL THE CERN FIRE BRIGADE

    CERN Multimedia

    2002-01-01

    The telephone numbers of the CERN Fire Brigade are: 74444 for emergency calls 74848 for other calls Note The number 112 will stay in use for emergency calls from 'wired' telephones, however, from mobile phones it leads to non-CERN emergency services.  

  12. HOW TO CALL THE CERN FIRE BRIGADE

    CERN Multimedia

    2001-01-01

    The telephone numbers of the CERN Fire Brigade are: 74444 for emergency calls 74848 for other calls Note: the number 112 will stay in use for emergency calls from 'wired' telephones, however, from mobile phones it leads to non-CERN emergency services.

  13. HOW TO CALL THE CERN FIRE BRIGADE

    CERN Document Server

    2001-01-01

    The telephone numbers of the CERN Fire Brigade are: 74444 for emergency calls 74848 for other calls Note The number 112 will stay in use for emergency calls from 'wired' telephones, however, from mobile phones it leads to non-CERN emergency services.

  14. How to call the Fire Brigade

    CERN Multimedia

    2003-01-01

    The telephone numbers for the CERN Fire Brigade are: 74444 for emergency calls 74848 for other calls Note The number 112 will stay in use for emergency calls from "wired" telephones, however, from mobile phones it leads to non-CERN emergency services.

  15. Do market participants learn from conference calls?

    NARCIS (Netherlands)

    Roelofsen, E.; Verbeeten, F.; Mertens, G.

    2014-01-01

    We examine whether market participants learn from the information that is disseminated during the Q-and-A section of conference calls. Specifically, we investigate whether stock prices react to information on intangible assets provided during conference calls, and whether conference calls interactio

  16. QSAR model for human pregnane X receptor (PXR) binding: Screening of environmental chemicals and correlations with genotoxicity, endocrine disruption and teratogenicity

    Energy Technology Data Exchange (ETDEWEB)

    Dybdahl, Marianne, E-mail: mdyb@food.dtu.dk; Nikolov, Nikolai G.; Wedebye, Eva Bay; Jónsdóttir, Svava Ósk; Niemelä, Jay R.

    2012-08-01

    The pregnane X receptor (PXR) has a key role in regulating the metabolism and transport of structurally diverse endogenous and exogenous compounds. Activation of PXR has the potential to initiate adverse effects, causing drug–drug interactions, and perturbing normal physiological functions. Therefore, identification of PXR ligands would be valuable information for pharmaceutical and toxicological research. In the present study, we developed a quantitative structure–activity relationship (QSAR) model for the identification of PXR ligands using data based on a human PXR binding assay. A total of 631 molecules, representing a variety of chemical structures, constituted the training set of the model. Cross-validation of the model showed a sensitivity of 82%, a specificity of 85%, and a concordance of 84%. The developed model provided knowledge about molecular descriptors that may influence the binding of molecules to PXR. The model was used to screen a large inventory of environmental chemicals, of which 47% was found to be within domain of the model. Approximately 35% of the chemicals within domain were predicted to be PXR ligands. The predicted PXR ligands were found to be overrepresented among chemicals predicted to cause adverse effects, such as genotoxicity, teratogenicity, estrogen receptor activation and androgen receptor antagonism compared to chemicals not causing these effects. The developed model may be useful as a tool for predicting potential PXR ligands and for providing mechanistic information of toxic effects of chemicals. -- Highlights: ► Global QSAR model for the identification of PXR ligands was developed. ► Molecular descriptors that may influence PXR binding were identified. ► 35% of a large set of environmental chemicals were predicted to be PXR ligands. ► Predicted PXR binding was associated with various adverse effects.

  17. Omeprazole and lansoprazole enantiomers induce CYP3A4 in human hepatocytes and cell lines via glucocorticoid receptor and pregnane X receptor axis.

    Science.gov (United States)

    Novotna, Aneta; Dvorak, Zdenek

    2014-01-01

    Benzimidazole drugs lansoprazole and omeprazole are used for treatment of various gastrointestinal pathologies. Both compounds cause drug-drug interactions because they activate aryl hydrocarbon receptor and induce CYP1A genes. In the current paper, we examined the effects of lansoprazole and omeprazole enantiomers on the expression of key drug-metabolizing enzyme CYP3A4 in human hepatocytes and human cancer cell lines. Lansoprazole enantiomers, but not omeprazole, were equipotent inducers of CYP3A4 mRNA in HepG2 cells. All forms (S-, R-, rac-) of lansoprazole and omeprazole induced CYP3A4 mRNA and protein in human hepatocytes. The quantitative profiles of CYP3A4 induction by individual forms of lansoprazole and omeprazole exerted enantiospecific patterns. Lansoprazole dose-dependently activated pregnane X receptor PXR in gene reporter assays, and slightly modulated rifampicin-inducible PXR activity, with similar potency for each enantiomer. Omeprazole dose-dependently activated PXR and inhibited rifampicin-inducible PXR activity. The effects of S-omeprazole were much stronger as compared to those of R-omeprazole. All forms of lansoprazole, but not omeprazole, slightly activated glucocorticoid receptor and augmented dexamethasone-induced GR transcriptional activity. Omeprazole and lansoprazole influenced basal and ligand inducible expression of tyrosine aminotransferase, a GR-target gene, in HepG2 cells and human hepatocytes. Overall, we demonstrate here that omeprazole and lansoprazole enantiomers induce CYP3A4 in HepG2 cells and human hepatocytes. The induction comprises differential interactions of omeprazole and lansoprazole with transcriptional regulators PXR and GR, and some of the effects were enantiospecific. The data presented here might be of toxicological and clinical importance, since the effects occurred in therapeutically relevant concentrations.

  18. Methadone induces the expression of hepatic drug-metabolizing enzymes through the activation of pregnane X receptor and constitutive androstane receptor.

    Science.gov (United States)

    Tolson, Antonia H; Li, Haishan; Eddington, Natalie D; Wang, Hongbing

    2009-09-01

    Methadone (MD) is the most established substance abuse pharmacotherapy of choice for the management of heroin dependence. To date, drug-drug interactions involving MD have been characterized asymmetrically among existing reports, which describe how other drugs affect the metabolic or pharmacokinetic profiles of MD; however, limited information is available regarding the potential for MD to influence similar fates of coadministered drugs. Moreover, little to no mechanistic evidence has been explored. Here, we show that MD induces hepatic drug-metabolizing enzymes (DMEs) through the activation of pregnane X receptor (PXR) and constitutive androstane receptor (CAR). Real-time polymerase chain reaction analysis of human hepatocyte cultures revealed that MD induces the mRNA expression of CYP2B6, CYP3A4, UGT1A1, and multidrug resistance 1 in a concentration-related manner, with the maximal induction of CYP2B6 challenging that of the induction by rifampicin. Furthermore, MD-mediated induction of CYP2B6 and CYP3A4 proteins was observed in Western blot analysis. In cell-based reporter assays, MD significantly increased human (h) PXR-mediated CYP2B6 reporter activities but exhibited minimal effect on hCAR activation as a result of the constitutive activity of hCAR in HepG2 cells. Further studies revealed that treatment with MD resulted in significant nuclear accumulation of adenovirus/enhanced yellow fluorescent protein tagged-hCAR in human hepatocytes, which has been regarded as the initial step of CAR activation. Additional analysis of the two enantiomers of MD, R-(-)-MD (active) and S-(+)-MD (inactive), indicates the lack of stereoselectivity pertaining to MD-mediated DME induction. Overall, our results show that MD induces the hepatic expression of multiple DMEs by activating PXR- and CAR-mediated pathways.

  19. Activation of pregnane X receptor by pregnenolone 16 α-carbonitrile prevents high-fat diet-induced obesity in AKR/J mice.

    Science.gov (United States)

    Ma, Yongjie; Liu, Dexi

    2012-01-01

    Pregnane X receptor (PXR) is known to function as a xenobiotic sensor to regulate xenobiotic metabolism through selective transcription of genes responsible for maintaining physiological homeostasis. Here we report that the activation of PXR by pregnenolone 16α-carbonitrile (PCN) in AKR/J mice can prevent the development of high-fat diet-induced obesity and insulin resistance. The beneficial effects of PCN treatment are seen with reduced lipogenesis and gluconeogenesis in the liver, and lack of hepatic accumulation of lipid and lipid storage in the adipose tissues. RT-PCR analysis of genes involved in gluconeogenesis, lipid metabolism and energy homeostasis reveal that PCN treatment on high-fat diet-fed mice reduces expression in the liver of G6Pase, Pepck, Cyp7a1, Cd36, L-Fabp, Srebp, and Fas genes and slightly enhances expression of Cyp27a1 and Abca1 genes. RT-PCR analysis of genes involved in adipocyte differentiation and lipid metabolism in white adipose tissue show that PCN treatment reduces expression of Pparγ2, Acc1, Cd36, but increases expression of Cpt1b and Pparα genes in mice fed with high-fat diet. Similarly, PCN treatment of animals on high-fat diet increases expression in brown adipose tissue of Pparα, Hsl, Cpt1b, and Cd36 genes, but reduces expression of Acc1 and Scd-1 genes. PXR activation by PCN in high-fat diet fed mice also increases expression of genes involved in thermogenesis in brown adipose tissue including Dio2, Pgc-1α, Pgc-1β, Cidea, and Ucp-3. These results verify the important function of PXR in lipid and energy metabolism and suggest that PXR represents a novel therapeutic target for prevention and treatment of obesity and insulin resistance.

  20. Rifaximin, a non-absorbable antibiotic, inhibits the release of pro-angiogenic mediators in colon cancer cells through a pregnane X receptor-dependent pathway.

    Science.gov (United States)

    Esposito, Giuseppe; Gigli, Stefano; Seguella, Luisa; Nobile, Nicola; D'Alessandro, Alessandra; Pesce, Marcella; Capoccia, Elena; Steardo, Luca; Cirillo, Carla; Cuomo, Rosario; Sarnelli, Giovanni

    2016-08-01

    Activation of intestinal human pregnane X receptor (PXR) has recently been proposed as a promising strategy for the chemoprevention of inflammation-induced colon cancer. The present study was aimed at evaluating the effect of rifaximin, a non-absorbable antibiotic, in inhibiting angiogenesis in a model of human colorectal epithelium and investigating the role of PXR in its mechanism of action. Caco-2 cells were treated with rifaximin (0.1, 1.0 and 10.0 µM) in the presence or absence of ketoconazole (10 µM) and assessed for cell proliferation, migration and expression of proliferating cell nuclear antigen (PCNA). The release of vascular endothelial growth factor (VEGF) and nitric oxide (NO), expression of Akt, mechanistic target of rapamycin (mTOR), p38 mitogen activated protein kinases (MAPK), nuclear factor κB (NF-κB) and metalloproteinase-2 and -9 (MMP-2 and -9) were also evaluated. Treatment with rifaximin 0.1, 1.0 and 10.0 µM caused significant and concentration-dependent reduction of cell proliferation, cell migration and PCNA expression in the Caco-2 cells vs. untreated cells. Treatment downregulated VEGF secretion, NO release, VEGFR-2 expression, MMP-2 and MMP-9 expression vs. untreated cells. Rifaximin treatment also resulted in a concentration-dependent decrease in the phosphorylation of Akt, mTOR, p38MAPK and inhibition of hypoxia-inducible factor 1-α (HIF-1α), p70S6K and NF-κB. Ketoconazole (PXR antagonist) treatment inhibited these effects. These findings demonstrated that rifaximin causes PXR-mediated inhibition of angiogenic factors in Caco-2 cell line and may be a promising anticancer tool.

  1. Pregnane X receptor agonists enhance intestinal epithelial wound healing and repair of the intestinal barrier following the induction of experimental colitis.

    Science.gov (United States)

    Terc, Joshua; Hansen, Ashleigh; Alston, Laurie; Hirota, Simon A

    2014-05-13

    The intestinal epithelial barrier plays a key role in the maintenance of homeostasis within the gastrointestinal tract. Barrier dysfunction leading to increased epithelial permeability is associated with a number of gastrointestinal disorders including the inflammatory bowel diseases (IBD) - Crohn's disease and ulcerative colitis. It is thought that the increased permeability in patients with IBD may be driven by alterations in the epithelial wound healing response. To this end considerable study has been undertaken to identify signaling pathways that may accelerate intestinal epithelial wound healing and normalize the barrier dysfunction observed in IBD. In the current study we examined the role of the pregnane X receptor (PXR) in modulating the intestinal epithelial wound healing response. Mutations and reduced mucosal expression of the PXR are associated with IBD, and others have reported that PXR agonists can dampen intestinal inflammation. Furthermore, stimulation of the PXR has been associated with increased cell migration and proliferation, two of the key processes involved in wound healing. We hypothesized that PXR agonists would enhance intestinal epithelial repair. Stimulation of Caco-2 intestinal epithelial cells with rifaximin, rifampicin and SR12813, all potent agonists of the PXR, significantly increased wound closure. This effect was driven by p38 MAP kinase-dependent cell migration, and occurred in the absence of cell proliferation. Treating mice with a rodent specific PXR agonist, pregnenolone 16α-carbonitrile (PCN), attenuated the intestinal barrier dysfunction observed in the dextran sulphate sodium (DSS) model of experimental colitis, an effect that occurred independent of the known anti-inflammatory effects of PCN. Taken together our data indicate that the activation of the PXR can enhance intestinal epithelial repair and suggest that targeting the PXR may help to normalize intestinal barrier dysfunction observed in patients with IBD

  2. Expedient synthesis of novel pregnane-NSAIDs prodrugs, XRD, stereochemistry of their C-20 derivatives by circular dichroism, conformational analysis, their DFT and TD-DFT studies

    Science.gov (United States)

    Singh, Ranvijay Pratap; Sharma, Sonia; Kant, Rajni; Amandeep; Singh, Praveer; Sethi, Arun

    2016-02-01

    Four novel pregnane-NSAIDs prodrugs 3β-(2-(6-methoxynaphthalene-2yl) propionoxy)-16α-methoxy-pregn-5-ene-20-one (3), 16α-methoxy-pregn-5-ene-20-one-3yl-2(4-iso butyl phenyl) propanoate (4), 3β-(2-(6-methoxynaphthalene-2yl) propionoxy) 20-hydroxy-16α-methoxy-pregn-5-ene (5) and 20-hydroxy-16α-methoxy-pregn-5-ene-20-one-3yl-2(4-iso butyl phenyl) propanoate (6) have been synthesized. They were analyzed experimentally by spectroscopic techniques like 1H, 13C NMR, FT-IR, UV-visible spectroscopy, mass spectrometry and correlated by theoretical calculations. The structure and conformations of 3 was established by single crystal X-ray diffraction, which crystallized in orthorhombic form having P212121 space group. Absolute configuration of C-20 hydroxy derivatives 5 and 6 was established by circular dichroism (CD) analysis. Conformational analysis of 5 was carried out to determine the most stable conformation. The electronic properties, such as frontier orbitals, band gap energies, oscillator strength and wavelength have been calculated using time dependent density functional theory (TD-DFT). The vibrational wavenumbers have been calculated using DFT method and assigned with the help of potential energy distribution (PED). Global and local reactivity descriptors have been computed to predict reactivity and reactive sites in the molecule. First hyperpolarizability (β0) of synthesized compounds has been computed to evaluate non-linear optical (NLO) response. Molecular electrostatic potential (MEP) for synthesized compounds have also been determined to check their electrophilic or nucleophilic reactivity as well as reaction path.

  3. A food contaminant ochratoxin A suppresses pregnane X receptor (PXR)-mediated CYP3A4 induction in primary cultures of human hepatocytes.

    Science.gov (United States)

    Doricakova, Aneta; Vrzal, Radim

    2015-11-04

    Ochratoxin A (OCHA) is a mycotoxin, which can be found in food such as coffee, wine, cereals, meat, nuts. Since it is absorbed via gastrointestinal tract, it is reasonable to anticipate that the liver will be the first organ to which OCHA comes into the contact before systemic circulation. Many xenobiotics are metabolically modified after the passage of the liver to biologically more active substances, sometimes with more harmful activity. Promoting own metabolism is often achieved via transcriptional regulation of biotransformation enzymes through ligand-activated transcription factors. Pregnane X receptor (PXR) belongs to such a group of regulators and it was demonstrated to be activated by many compounds of synthetic as well as natural origin. Our intention was to investigate if OCHA is capable of activating the PXR with consequent induction of PXR-regulated CYP3A4 gene. We found that OCHA does not activate PXR but displays antagonist-like behavior when combined with rifampicin (RIF) in gene reporter assay in human embryonal kidney cells (Hek293T). It was very weak inducer of CYP3A4 mRNA in primary cultures of human hepatocytes and it antagonized RIF-mediated CYP3A4 induction of mRNA as well as protein. In addition, it caused the decline of PXR protein as well as mRNA which was faster than that with actinomycin D, a transcription inhibitor. Since we found that OCHA induced the expression of miR-148a, which was described to regulate PXR expression, we conclude that antagonist-like behavior of OCHA is not due to the antagonism itself but due to the downregulation of PXR gene expression. Herein we provide important findings which bring a piece of puzzle into the understanding of mechanism of toxic action of ochratoxin A.

  4. Carboxymefloquine, the major metabolite of the antimalarial drug mefloquine, induces drug-metabolizing enzyme and transporter expression by activation of pregnane X receptor.

    Science.gov (United States)

    Piedade, Rita; Traub, Stefanie; Bitter, Andreas; Nüssler, Andreas K; Gil, José P; Schwab, Matthias; Burk, Oliver

    2015-01-01

    Malaria patients are frequently coinfected with HIV and mycobacteria causing tuberculosis, which increases the use of coadministered drugs and thereby enhances the risk of pharmacokinetic drug-drug interactions. Activation of the pregnane X receptor (PXR) by xenobiotics, which include many drugs, induces drug metabolism and transport, thereby resulting in possible attenuation or loss of the therapeutic responses to the drugs being coadministered. While several artemisinin-type antimalarial drugs have been shown to activate PXR, data on nonartemisinin-type antimalarials are still missing. Therefore, this study aimed to elucidate the potential of nonartemisinin antimalarial drugs and drug metabolites to activate PXR. We screened 16 clinically used antimalarial drugs and six major drug metabolites for binding to PXR using the two-hybrid PXR ligand binding domain assembly assay; this identified carboxymefloquine, the major and pharmacologically inactive metabolite of the antimalarial drug mefloquine, as a potential PXR ligand. Two-hybrid PXR-coactivator and -corepressor interaction assays and PXR-dependent promoter reporter gene assays confirmed carboxymefloquine to be a novel PXR agonist which specifically activated the human receptor. In the PXR-expressing intestinal LS174T cells and in primary human hepatocytes, carboxymefloquine induced the expression of drug-metabolizing enzymes and transporters on the mRNA and protein levels. The crucial role of PXR for the carboxymefloquine-dependent induction of gene expression was confirmed by small interfering RNA (siRNA)-mediated knockdown of the receptor. Thus, the clinical use of mefloquine may result in pharmacokinetic drug-drug interactions by means of its metabolite carboxymefloquine. Whether these in vitro findings are of in vivo relevance has to be addressed in future clinical drug-drug interaction studies.

  5. Pyrethroid insecticides: isoform-dependent hydrolysis, induction of cytochrome P450 3A4 and evidence on the involvement of the pregnane X receptor.

    Science.gov (United States)

    Yang, Dongfang; Wang, Xiliang; Chen, Yi-Tzai; Deng, Ruitang; Yan, Bingfang

    2009-05-15

    Pyrethroids account for more than one-third of the insecticides currently marketed in the world. In mammals, these insecticides undergo extensive metabolism by carboxylesterases and cytochrome P450s (CYPs). In addition, some pyrethroids are found to induce the expression of CYPs. The aim of this study was to determine whether pyrethroids induce carboxylesterases and CYP3A4, and whether the induction is correlated inversely with their hydrolysis. Human liver microsomes were pooled and tested for the hydrolysis of 11 pyrethroids. All pyrethroids were hydrolyzed by the pooled microsomes, but the hydrolytic rates varied by as many as 14 fold. Some pyrethroids such as bioresmethrin were preferably hydrolyzed by carboxylesterase HCE1, whereas others such as bifenthrin preferably by HCE2. In primary human hepatocytes, all pyrethroids except tetramethrin significantly induced CYP3A4. In contrast, insignificant changes were detected on the expression of carboxylesterases. The induction of CYP3A4 was confirmed in multiple cell lines including HepG2, Hop92 and LS180. Overall, the magnitude of the induction was correlated inversely with the rates of hydrolysis, but positively with the activation of the pregnane X receptor (PXR). Transfection of a carboxylesterase markedly decreased the activation of PXR, and the decrease was in agreement with carboxylesterase-based preference for hydrolysis. In addition, human PXR variants as well as rat PXR differed from human PXR (wild-type) in responding to certain pyrethroids (e.g., lambda-cyhalothrin), suggesting that induction of PXR target genes by these pyrethroids varies depending on polymorphic variants and the PXR species identity.

  6. St. John's wort may ameliorate 2,4,6-trinitrobenzenesulfonic acid colitis off rats through the induction of pregnane X receptors and/or P-glycoproteins.

    Science.gov (United States)

    Sehirli, A O; Cetinel, S; Ozkan, N; Selman, S; Tetik, S; Yuksel, M; Dulger, F G A

    2015-04-01

    It is reported that deficiencies of the pregnane X receptor (PXR) and P-glycoprotein (P-gp), the latter of which is encoded by the MDR1 gene, are important factors in the pathogenesis of inflammatory bowel disease (IBD). It is also known that the activation of PXR is protective of IBD due to the mutual repression between PXR and nuclear factor kappa B (NF-κB) expression and because NF-κB was reported to play a pivotal role in the pathogenesis of ulcerative colitis. The goal of this study was to investigate whether St. John's wort (SJW) and spironolactone (SPL), both known to have strong inducing effects on cytochrome P 450 (CYP) enzymes as well as PXR and P-gp, have ameliorating effects on 2,4,6-trinitrobenzenesulfonic acid (TNBS) colitis of rats through induction of PXR and/or P-gp. Wistar albino rats (250 - 300 g) were divided into control and TNBS-colitis groups. Each group was then divided into a) control (saline), b) SJW (300 mg/kg p.o. bid), and c) SPL (80 mg/kg p.o.) groups. Drugs were given for 7 days. Both treatments ameliorated the clinical hallmarks of colitis, as determined by body weight loss and assessment of diarrhea, colon length, and bowel histology. Plasma levels of NF-κB, tumour necrosis factor-alpha (TNF-α) and tissue myeloperoxidase (MPO) activity, as well as the oxidative stress markers that increased during colitis, decreased significantly after both treatments. The PXR and P-gp expression in the intestinal tissues was diminished in the colitis group but increased after drug treatments. Both drugs appeared to have significant antioxidant and anti-inflammatory effects and ameliorated the TNBS colitis of the rats, most likely through their PXR- and P-gp-inducing properties.

  7. Activation of pregnane X receptor by pregnenolone 16 α-carbonitrile prevents high-fat diet-induced obesity in AKR/J mice.

    Directory of Open Access Journals (Sweden)

    Yongjie Ma

    Full Text Available Pregnane X receptor (PXR is known to function as a xenobiotic sensor to regulate xenobiotic metabolism through selective transcription of genes responsible for maintaining physiological homeostasis. Here we report that the activation of PXR by pregnenolone 16α-carbonitrile (PCN in AKR/J mice can prevent the development of high-fat diet-induced obesity and insulin resistance. The beneficial effects of PCN treatment are seen with reduced lipogenesis and gluconeogenesis in the liver, and lack of hepatic accumulation of lipid and lipid storage in the adipose tissues. RT-PCR analysis of genes involved in gluconeogenesis, lipid metabolism and energy homeostasis reveal that PCN treatment on high-fat diet-fed mice reduces expression in the liver of G6Pase, Pepck, Cyp7a1, Cd36, L-Fabp, Srebp, and Fas genes and slightly enhances expression of Cyp27a1 and Abca1 genes. RT-PCR analysis of genes involved in adipocyte differentiation and lipid metabolism in white adipose tissue show that PCN treatment reduces expression of Pparγ2, Acc1, Cd36, but increases expression of Cpt1b and Pparα genes in mice fed with high-fat diet. Similarly, PCN treatment of animals on high-fat diet increases expression in brown adipose tissue of Pparα, Hsl, Cpt1b, and Cd36 genes, but reduces expression of Acc1 and Scd-1 genes. PXR activation by PCN in high-fat diet fed mice also increases expression of genes involved in thermogenesis in brown adipose tissue including Dio2, Pgc-1α, Pgc-1β, Cidea, and Ucp-3. These results verify the important function of PXR in lipid and energy metabolism and suggest that PXR represents a novel therapeutic target for prevention and treatment of obesity and insulin resistance.

  8. Serine 350 of human pregnane X receptor is crucial for its heterodimerization with retinoid X receptor alpha and transactivation of target genes in vitro and in vivo.

    Science.gov (United States)

    Wang, Yue-Ming; Chai, Sergio C; Lin, Wenwei; Chai, Xiaojuan; Elias, Ayesha; Wu, Jing; Ong, Su Sien; Pondugula, Satyanarayana R; Beard, Jordan A; Schuetz, Erin G; Zeng, Su; Xie, Wen; Chen, Taosheng

    2015-08-15

    The human pregnane X receptor (hPXR), a member of the nuclear receptor superfamily, senses xenobiotics and controls the transcription of genes encoding drug-metabolizing enzymes and transporters. The regulation of hPXR's transcriptional activation of its target genes is important for xenobiotic detoxification and endobiotic metabolism, and hPXR dysregulation can cause various adverse drug effects. Studies have implicated the putative phosphorylation site serine 350 (Ser(350)) in regulating hPXR transcriptional activity, but the mechanism of regulation remains elusive. Here we investigated the transactivation of hPXR target genes in vitro and in vivo by hPXR with a phosphomimetic mutation at Ser(350) (hPXR(S350D)). The S350D phosphomimetic mutation reduced the endogenous expression of cytochrome P450 3A4 (an hPXR target gene) in HepG2 and LS180 cells. Biochemical assays and structural modeling revealed that Ser(350) of hPXR is crucial for formation of the hPXR-retinoid X receptor alpha (RXRα) heterodimer. The S350D mutation abrogated heterodimerization in a ligand-independent manner, impairing hPXR-mediated transactivation. Further, in a novel humanized transgenic mouse model expressing the hPXR(S350D) transgene, we demonstrated that the S350D mutation alone is sufficient to impair hPXR transcriptional activity in mouse liver. This transgenic mouse model provides a unique tool to investigate the regulation and function of hPXR, including its non-genomic function, in vivo. Our finding that phosphorylation regulates hPXR activity has implications for development of novel hPXR antagonists and for safety evaluation during drug development.

  9. Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yue-Ming; Lin, Wenwei; Chai, Sergio C.; Wu, Jing; Ong, Su Sien [Department of Chemical Biology and Therapeutics, St. Jude Children' s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105 (United States); Schuetz, Erin G. [Department of Pharmaceutical Sciences, St. Jude Children' s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105 (United States); Chen, Taosheng, E-mail: taosheng.chen@stjude.org [Department of Chemical Biology and Therapeutics, St. Jude Children' s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105 (United States)

    2013-10-01

    Activation of the pregnane X receptor (PXR) and subsequently its target genes, including those encoding drug transporters and metabolizing enzymes, while playing substantial roles in xenobiotic detoxification, might cause undesired drug-drug interactions. Recently, an increased awareness has been given to dietary components for potential induction of diet–drug interactions through activation of PXR. Here, we studied, whether piperine (PIP), a major component extracted from the widely-used daily spice black pepper, could induce PXR-mediated expression of cytochrome P450 3A4 (CYP3A4) and multidrug resistance protein 1 (MDR1). Our results showed that PIP activated human PXR (hPXR)-mediated CYP3A4 and MDR1 expression in human hepatocytes, intestine cells, and a mouse model; PIP activated hPXR by recruiting its coactivator SRC-1 in both cellular and cell-free systems; PIP bound to the hPXR ligand binding domain in a competitive ligand binding assay in vitro. The dichotomous effects of PIP on induction of CYP3A4 and MDR1 expression observed here and inhibition of their activity reported elsewhere challenges the potential use of PIP as a bioavailability enhancer and suggests that caution should be taken in PIP consumption during drug treatment in patients, particularly those who favor daily pepper spice or rely on certain pepper remedies. - Highlights: • Piperine induces PXR-mediated CYP3A4 and MDR1 expression. • Piperine activates PXR by binding to PXR and recruiting coactivator SRC-1. • Piperine induces PXR activation in vivo. • Caution should be taken in piperine consumption during drug treatment.

  10. Static analysis of function calls in Erlang

    Directory of Open Access Journals (Sweden)

    Dániel Horpácsi

    2013-06-01

    Full Text Available Functions and their relations can affect numerous properties and metrics of a functional program. To identify and represent the functions and their calling connections, software analysers commonly apply semantic function analysis, which derives the static call graph of the program, based on its source code. Function calls however may be dynamic and complex, making it difficult to statically identify the callee. Dynamic calls are determined just at run-time, static analysis therefore cannot be expected to fully identify every call. Nevertheless, by utilising the results of a properly performed data-flow analysis as well as taking ambiguous references into account, numerous dynamic calls are discoverable and representable. We consider cases where the identifiers of the callee are statically determined, but they flow into the call expression from a different program point, and also, we manage to handle function calls whose identifiers are not fully identifiable at compile-time. By utilising the improved reference analysis, we extend the static call graph with various information about dynamic function calls. We investigate such a function call analysis in the programming language Erlang.

  11. Bonobos extract meaning from call sequences.

    Directory of Open Access Journals (Sweden)

    Zanna Clay

    Full Text Available Studies on language-trained bonobos have revealed their remarkable abilities in representational and communication tasks. Surprisingly, however, corresponding research into their natural communication has largely been neglected. We address this issue with a first playback study on the natural vocal behaviour of bonobos. Bonobos produce five acoustically distinct call types when finding food, which they regularly mix together into longer call sequences. We found that individual call types were relatively poor indicators of food quality, while context specificity was much greater at the call sequence level. We therefore investigated whether receivers could extract meaning about the quality of food encountered by the caller by integrating across different call sequences. We first trained four captive individuals to find two types of foods, kiwi (preferred and apples (less preferred at two different locations. We then conducted naturalistic playback experiments during which we broadcasted sequences of four calls, originally produced by a familiar individual responding to either kiwi or apples. All sequences contained the same number of calls but varied in the composition of call types. Following playbacks, we found that subjects devoted significantly more search effort to the field indicated by the call sequence. Rather than attending to individual calls, bonobos attended to the entire sequences to make inferences about the food encountered by a caller. These results provide the first empirical evidence that bonobos are able to extract information about external events by attending to vocal sequences of other individuals and highlight the importance of call combinations in their natural communication system.

  12. 孕烷X受体及组成性雄甾烷受体的研究新进展%Advances in the research of pregnane X receptor and constitutive androstane receptor

    Institute of Scientific and Technical Information of China (English)

    胡冰芳; 毕惠嫦; 黄民

    2011-01-01

    孕烷受体(pregnane X receptor,PXR)和组成性雄甾烷受体(constitutive androstane receptor,CAR)是核受体(nuclear receptor,NR)亚家族的重要成员;为配体活化的转录因子,能调控大量的靶基因.本文主要对其基本结构、机制及参与转录活化的辅助因子作简要介绍,重点讲述了它们在调节药物代谢与转运、糖异生及生酮作用、脂质代谢以及炎症反应等方面的意义.通过对PXR及CAR的研究,可以有效预测和防止药物相互作用;为寻找疾病治疗新靶标提供方向.%Nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are originally characterized as transcription factors regulating many target genes. Recent works have revealed that these nuclear receptors play critical roles in regulating genes that encode drug metabolism enzymes and modulating hepatic energy metabolism, such as down-regulating gluconeogenesis, fatty acid oxidation, and ketogenesis, as well as up-regulating lipogenesis. Studies on PXR and CAR have important implication on drug-drug interaction (DDI) and potential disease treatment targets.

  13. Multidimensional views on mobile call network

    Institute of Scientific and Technical Information of China (English)

    Shengqi YANG; Bin WU; Bai WANG

    2009-01-01

    Recent studies on social network have spurred significant interests in human behaviors. Nowadays, various kinds of interpersonal human interactions, from mobile calls to emalls, provide particular avenues to explore the inher-ent properties of communication patterns. In this article, we present a comprehensive study on a massive anonymous call records obtained from a major mobile service operator. The important difference laid in our work and previous mainly topological analyses is that we report on multiple aspects of the dataset. By investigating the calls of the users, we find out that most calls tend to last within one minute. Call du-ration between two females is much longer than that of two males. But calls of males generally involve more stations than that of female, indicating a larger mobile range of the males. We also observed that people tend to communicate more with each other when they share similar characters. Besides, the network is well-connected and robust to random attack. We also demonstrate that the close-knit sub-groups with little discrepancy in the characteristics of its involved users usually evoke more calls. Another interesting discov-ery is that call behaviors among people between workdays and weekends is obviously distinct. Generally speaking, the goal that we research on call network through multidimen-sional analyses is to uncover the intricate patterns of human communications and put up reasonable insights into future service intelligence.

  14. Call Duration Characteristics based on Customers Location

    Directory of Open Access Journals (Sweden)

    Žvinys Karolis

    2014-05-01

    Full Text Available Nowadays a lot of different researches are performed based on call duration distributions (CDD analysis. However, the majority of studies are linked with social relationships between the people. Therefore the scarcity of information, how the call duration is associated with a user's location, is appreciable. The goal of this paper is to reveal the ties between user's voice call duration and the location of call. For this reason we analyzed more than 5 million calls from real mobile network, which were made over the base stations located in rural areas, roads, small towns, business and entertainment centers, residential districts. According to these site types CDD’s and characteristic features for call durations are given and discussed. Submitted analysis presents the users habits and behavior as a group (not an individual. The research showed that CDD’s of customers being them in different locations are not equal. It has been found that users at entertainment, business centers are tend to talk much shortly, than people being at home. Even more CDD can be distorted strongly, when machinery calls are evaluated. Hence to apply a common CDD for a whole network it is not recommended. The study also deals with specific parameters of call duration for distinguished user groups, the influence of network technology for call duration is considered.

  15. When They Talk about CALL: Discourse in a Required CALL Class

    Science.gov (United States)

    Kessler, Greg

    2010-01-01

    This study investigates preservice teachers' discourse about CALL in a required CALL class which combines theory and practice. Thirty-three students in a Linguistics MA program CALL course were observed over a 10-week quarter. For all of these students, it was their first formal exposure to CALL as a discipline. Communication in the class…

  16. The Call to Teach and Teacher Hopefulness

    Science.gov (United States)

    Bullough, Robert V., Jr.; Hall-Kenyon, Kendra M.

    2011-01-01

    The purpose of this paper is to explore teacher motivation and well-being. Our analysis focuses on two central concepts, the notion of a "calling to teach" and of teacher "hopefulness." Data from 205 preservice and inservice teachers were collected to determine teachers' sense of calling and level of hope. Results indicate that overwhelmingly,…

  17. A CALL for Improved School Leadership

    Science.gov (United States)

    Halverson, Richard; Kelley, Carolyn; Shaw, James

    2014-01-01

    The Comprehensive Assessment of Leadership for Learning (CALL) is a formative assessment that provides feedback to schools on the research-based leadership practices necessary to improve teaching and learning. Instead of focusing on an individual leader, CALL measures leadership practices in tasks carried out by actors across the school and…

  18. Answering the "Call of the Mountain"

    NARCIS (Netherlands)

    Chaves Villegas, Martha

    2016-01-01

    In response to the age of the ‘anthropocene,’ as some authors are calling this epoch in which one single species is disrupting major natural systems (Steffen et al 2011), there are calls for more radical, learning-based sustainability that generates deep transformations in individuals an

  19. Integrated Language Skills CALL Course Design

    Science.gov (United States)

    Watson, Kevin; Agawa, Grant

    2013-01-01

    The importance of a structured learning framework or interrelated frameworks is the cornerstone of a solid English as a foreign language (EFL) computer-assisted language learning (CALL) curriculum. While the benefits of CALL are widely promoted in the literature, there is often an endemic discord separating theory and practice. Oftentimes the…

  20. 33 CFR 401.64 - Calling in.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Calling in. 401.64 Section 401.64... SEAWAY REGULATIONS AND RULES Regulations Radio Communications § 401.64 Calling in. (a) Every vessel, intending to transit or in transit, shall report on the assigned frequency to the designated Seaway...

  1. Answering the "Call of the Mountain"

    NARCIS (Netherlands)

    Chaves Villegas, Martha

    2016-01-01

    In response to the age of the ‘anthropocene,’ as some authors are calling this epoch in which one single species is disrupting major natural systems (Steffen et al 2011), there are calls for more radical, learning-based sustainability that generates deep transformations in individuals

  2. 78 FR 76218 - Rural Call Completion

    Science.gov (United States)

    2013-12-17

    ... providers of VoIP service, other providers could circumvent the rules by working with a VoIP service... expect the Commission will obtain the data we need to identify and analyze patterns of call completion... below will allow the Commission to better identify patterns of rural call completion problems and...

  3. CALL in Lebanese Elementary ESL Writing Classrooms

    Science.gov (United States)

    Fidaoui, Diana; Bahous, Rima; Bacha, Nahla N.

    2010-01-01

    This article discusses the effectiveness of using computer assisted language learning (CALL) in motivating fourth-grade English as a second language (ESL) students to develop better writing skills. It also seeks to explore the perceptions of ESL teachers and students regarding the use of CALL in the ESL classroom. The study involved 48 fourth…

  4. Communication cliques in mobile phone calling networks

    CERN Document Server

    Li, Ming-Xia; Jiang, Zhi-Qiang; Zhou, Wei-Xing

    2015-01-01

    People in modern societies form different social networks through numerous means of communication. These communication networks reflect different aspects of human's societal structure. The billing records of calls among mobile phone users enable us to construct a directed calling network (DCN) and its Bonferroni network (SVDCN) in which the preferential communications are statistically validated. Here we perform a comparative investigation of the cliques of the original DCN and its SVDCN constructed from the calling records of more than nine million individuals in Shanghai over a period of 110 days. We find that the statistical properties of the cliques of the two calling networks are qualitatively similar and the clique members in the DCN and the SVDCN exhibit idiosyncratic behaviors quantitatively. Members in large cliques are found to be spatially close to each other. Based on the clique degree profile of each mobile phone user, the most active users in the two calling networks can be classified in to seve...

  5. Call Admission Control in Mobile Cellular Networks

    CERN Document Server

    Ghosh, Sanchita

    2013-01-01

    Call Admission Control (CAC) and Dynamic Channel Assignments (DCA) are important decision-making problems in mobile cellular communication systems. Current research in mobile communication considers them as two independent problems, although the former greatly depends on the resulting free channels obtained as the outcome of the latter. This book provides a solution to the CAC problem, considering DCA as an integral part of decision-making for call admission. Further, current technical resources ignore movement issues of mobile stations and fluctuation in network load (incoming calls) in the control strategy used for call admission. In addition, the present techniques on call admission offers solution globally for the entire network, instead of considering the cells independently.      CAC here has been formulated by two alternative approaches. The first approach aimed at handling the uncertainty in the CAC problem by employing fuzzy comparators.  The second approach is concerned with formulation of CAC ...

  6. Call cultures in orang-utans?

    Directory of Open Access Journals (Sweden)

    Serge A Wich

    Full Text Available BACKGROUND: Several studies suggested great ape cultures, arguing that human cumulative culture presumably evolved from such a foundation. These focused on conspicuous behaviours, and showed rich geographic variation, which could not be attributed to known ecological or genetic differences. Although geographic variation within call types (accents has previously been reported for orang-utans and other primate species, we examine geographic variation in the presence/absence of discrete call types (dialects. Because orang-utans have been shown to have geographic variation that is not completely explicable by genetic or ecological factors we hypothesized that this will be similar in the call domain and predict that discrete call type variation between populations will be found. METHODOLOGY/PRINCIPAL FINDINGS: We examined long-term behavioural data from five orang-utan populations and collected fecal samples for genetic analyses. We show that there is geographic variation in the presence of discrete types of calls. In exactly the same behavioural context (nest building and infant retrieval, individuals in different wild populations customarily emit either qualitatively different calls or calls in some but not in others. By comparing patterns in call-type and genetic similarity, we suggest that the observed variation is not likely to be explained by genetic or ecological differences. CONCLUSION/SIGNIFICANCE: These results are consistent with the potential presence of 'call cultures' and suggest that wild orang-utans possess the ability to invent arbitrary calls, which spread through social learning. These findings differ substantially from those that have been reported for primates before. First, the results reported here are on dialect and not on accent. Second, this study presents cases of production learning whereas most primate studies on vocal learning were cases of contextual learning. We conclude with speculating on how these findings might

  7. Pregnane X Receptor Not Nuclear Factor-kappa B Up-regulates P-glycoprotein Expression in the Brain of Chronic Epileptic Rats Induced by Kainic Acid.

    Science.gov (United States)

    Yu, Nian; Zhang, Yan-Fang; Zhang, Kang; Cheng, Yong-Fei; Ma, Hai-Yan; Di, Qing

    2017-03-16

    Drug-resistance epilepsy (DRE) is attributed to the brain P-glycoprotein (P-gp) overexpression. We previously reported that nuclear factor-kappa B (NF-κB) played a critical role in regulating P-gp expression at the brain of the acute seizure rats. This study was extended further to investigate the interaction effect of NF-κB and pregnane X receptor (PXR) on P-gp expression at the brain of chronic epileptic rats treated with carbamazepine (CBZ). The chronic epileptic models were induced by the micro-injection of kainic acid (KA) into rats' hippocampus. Subsequently, the successful models were treated with different intervention agents of CBZ; PMA(a non-specific PXR activity inhibitor) or PDTC(a specific NF-κB activity inhibitor) respectively. The expression levels of P-gp and its encoded gene mdr1a/b were significantly up-regulated on the brain of KA-induced chronic epilepsy rats or the epilepsy rats treated with CBZ for 1 week, meanwhile with a high expression of PXR. The treatment of PMA dramatically reduced both PXR and P-gp expressions at the protein and mRNA levels in the chronic epilepsy brain. By compared to the epilepsy model group, the P-gp expression was not markedly attenuated by the inhibition of NF-κB activity with PDTC treatment, nevertheless with a decrease of NF-κB expression in this intervention group. Higher levels of proinflammatory cytokines(IL-1β, IL-6, TNF-α) were found both in the brain tissue and the serum in the epilepsy rats of each group. There was a declined trend of the pro-inflammatory cytokines expression of the PDTC treatment group but with no statistical significance. This study demonstrates for the first time that P-gp up-regulation is due to increase PXR expression in the chronic phase of epilepsy, differently from that NF-κB signaling may induce the P-gp expression in the acute seizure phase. Our results offer insights into the mechanism underlying the development of DRE using or not using CBZ treatment.

  8. Pregnane X receptors regulate CYP2C8 and P-glycoprotein to impact on the resistance of NSCLC cells to Taxol.

    Science.gov (United States)

    Chen, Yan; Huang, Wandan; Chen, Feiyu; Hu, Guoping; Li, Fenglei; Li, Jianhua; Xuan, Aiguo

    2016-12-01

    Cytochrome P450 2C8 (CYP2C8) is one of the enzymes that primarily participate in producing metabolisms of medications and P-glycoprotein (P-gp) has been regarded as one of the important molecules in chemotherapeutically induced multidrug resistance (MDR). In addition, the pregnane X receptor (PXR) is involved in regulating both CYP2C8 and P-gp. We aim to research the effect of PXR on Taxol-resistant non-small-cell lung cancer (NSCLC cells) via regulating CYP2C8 and P-gp. NSCLC cells were treated with SR12813, LY335979, or PXR siRNA. Cell counting kit (CCK-8) assay was used to detect cell vitality. Colony formation assay was used to observe cell proliferation. Western blotting, real-time polymerase chain reaction (RT-PCR), and immunofluorescence staining were conducted to analyze the expressions of PXR, CYP2C8, and P-gp. Taxol and its metabolic products were detected by high-performance liquid chromatography (HPLC). The expression of PXR in A549 cell line was higher than that in other cell lines. The accumulation of PXR was observed in the nucleus after cells were treated with SR12813. Besides, SR12813 induced higher expressions of CYP2C8 and P-gp proteins. We also discovered that pretreatment with SR12813 reversed the inhibition of cell viability and proliferation after the Taxol treatment in comparison to the SR12813 untreated group. Furthermore, the hydroxylation products of Taxol analyzed by HPLC were increased in comparison to the SR12813 untreated group, indicating that high expressions of CYP2C8 and P-gp enhanced the resistance of A549 cells to Taxol. For cells treated with PXR siRNA, cell viability, cell proliferation, and Taxol metabolites were significantly reduced after the Taxol treatment in comparison to the siRNA-negative group. The cell viability, cell proliferation, and Taxol metabolites were regulated by the expressions of PXR, P-gp, and CYP2C8. That is, PXR expression has an important effect on the resistance of NSCLC cells to Taxol via

  9. The evolution of farnesoid X, vitamin D, and pregnane X receptors: insights from the green-spotted pufferfish (Tetraodon nigriviridis and other non-mammalian species

    Directory of Open Access Journals (Sweden)

    Kullman Seth W

    2011-02-01

    Full Text Available Abstract Background The farnesoid X receptor (FXR, pregnane X receptor (PXR, and vitamin D receptor (VDR are three closely related nuclear hormone receptors in the NR1H and 1I subfamilies that share the property of being activated by bile salts. Bile salts vary significantly in structure across vertebrate species, suggesting that receptors binding these molecules may show adaptive evolutionary changes in response. We have previously shown that FXRs from the sea lamprey (Petromyzon marinus and zebrafish (Danio rerio are activated by planar bile alcohols found in these two species. In this report, we characterize FXR, PXR, and VDR from the green-spotted pufferfish (Tetraodon nigriviridis, an actinopterygian fish that unlike the zebrafish has a bile salt profile similar to humans. We utilize homology modelling, docking, and pharmacophore studies to understand the structural features of the Tetraodon receptors. Results Tetraodon FXR has a ligand selectivity profile very similar to human FXR, with strong activation by the synthetic ligand GW4064 and by the primary bile acid chenodeoxycholic acid. Homology modelling and docking studies suggest a ligand-binding pocket architecture more similar to human and rat FXRs than to lamprey or zebrafish FXRs. Tetraodon PXR was activated by a variety of bile acids and steroids, although not by the larger synthetic ligands that activate human PXR such as rifampicin. Homology modelling predicts a larger ligand-binding cavity than zebrafish PXR. We also demonstrate that VDRs from the pufferfish and Japanese medaka were activated by small secondary bile acids such as lithocholic acid, whereas the African clawed frog VDR was not. Conclusions Our studies provide further evidence of the relationship between both FXR, PXR, and VDR ligand selectivity and cross-species variation in bile salt profiles. Zebrafish and green-spotted pufferfish provide a clear contrast in having markedly different primary bile salt profiles

  10. Functional characterization of a full length pregnane X receptor, expression in vivo, and identification of PXR alleles, in Zebrafish (Danio rerio)

    Energy Technology Data Exchange (ETDEWEB)

    Bainy, Afonso C.D. [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Departamento de Bioquímica, CCB, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900 (Brazil); Kubota, Akira; Goldstone, Jared V. [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Lille-Langøy, Roger [Department of Biology, University of Bergen, N-5020 Bergen (Norway); Karchner, Sibel I. [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Celander, Malin C. [Department of Biological and Environmental Sciences, University of Gothenburg, SE 405 30 Göteborg (Sweden); Hahn, Mark E. [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Goksøyr, Anders [Department of Biology, University of Bergen, N-5020 Bergen (Norway); Stegeman, John J., E-mail: jstegeman@whoi.edu [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States)

    2013-10-15

    Highlights: •Full-length pxr has been cloned from zebrafish. •Alleles of pxr were identified in zebrafish. •Full length Pxr was activated less strongly than ligand binding domain in cell-based reporter assays. •High levels of pxr expression were found in eye and brain as well as in liver. •TCPOBOP and PB did not significantly alter expression of pxr in liver. -- Abstract: The pregnane X receptor (PXR) (nuclear receptor NR1I2) is a ligand activated transcription factor, mediating responses to diverse xenobiotic and endogenous chemicals. The properties of PXR in fish are not fully understood. Here we report on cloning and characterization of full-length PXR of zebrafish, Danio rerio, and pxr expression in vivo. Initial efforts gave a cDNA encoding a 430 amino acid protein identified as zebrafish pxr by phylogenetic and synteny analysis. The sequence of the cloned Pxr DNA binding domain (DBD) was highly conserved, with 74% identity to human PXR-DBD, while the ligand-binding domain (LBD) of the cloned sequence was only 44% identical to human PXR-LBD. Sequence variation among clones in the initial effort prompted sequencing of multiple clones from a single fish. There were two prominent variants, one sequence with S183, Y218 and H383 and the other with I183, C218 and N383, which we designate as alleles pxr*1 (nr1i2*1) and pxr*2 (nr1i2*2), respectively. In COS-7 cells co-transfected with a PXR-responsive reporter gene, the full-length Pxr*1 (the more common variant) was activated by known PXR agonists clotrimazole and pregnenolone 16α-carbonitrile but to a lesser extent than the full-length human PXR. Activation of full-length Pxr*1 was only 10% of that with the Pxr*1 LBD. Quantitative real time PCR analysis showed prominent expression of pxr in liver and eye, as well as brain and intestine of adult zebrafish. The pxr was expressed in heart and kidney at levels similar to that in intestine. The expression of pxr in liver was weakly induced by ligands for

  11. External GSM phone calls now made simpler

    CERN Multimedia

    2007-01-01

    On 2 July, the IT/CS Telecom Service introduced a new service making external calls from CERN GSM phones easier. A specific prefix is no longer needed for calls outside CERN. External calls from CERN GSM phones are to be simplified. It is no longer necessary to use a special prefix to call an external number from the CERN GSM network.The Telecom Section of the IT/CS Group is introducing a new system that will make life easier for GSM users. It is no longer necessary to use a special prefix (333) to call an external number from the CERN GSM network. Simply dial the number directly like any other Swiss GSM customer. CERN currently has its own private GSM network with the Swiss mobile operator, Sunrise, covering the whole of Switzerland. This network was initially intended exclusively for calls between CERN numbers (replacing the old beeper system). A special system was later introduced for external calls, allowing them to pass thr...

  12. Arginine vasotocin promotes calling behavior and call changes in male túngara frogs.

    Science.gov (United States)

    Kime, Nicole M; Whitney, Tina K; Davis, Ellen S; Marler, Catherine A

    2007-01-01

    In the túngara frog, Physalaemus pustulosus, males alter calling behavior with changes in their social environment, adding 'chucks' to their advertisement calls in response to the calls of conspecific males. Other studies demonstrate that adding chucks increases the attractiveness of calls to females but also increases the risk of bat predation. In the current study, subcutaneous injections of the neuropeptide hormone arginine vasotocin (AVT) significantly increased chuck production in male túngara frogs. The effects of AVT on chuck production did not depend on the presence of playback stimuli, suggesting that AVT increased either the males' general motivation to produce chucks or their responsiveness to the calls of distant males. Injections of AVT also increased the probability that males would call and decreased the latency to call after injection, supporting the hypothesis that AVT influences motivation to call. Finally, AVT inhibited a drop in call rate after the termination of a playback stimulus and increased call rate at a lower dose of AVT. The effects of AVT on chucks and call rate appear to be independent of each other, as there was no correlation between change in chuck production and change in call rate in individual males. We conclude that AVT may play an important role in socially-mediated call changes that result from competition for mates. The behavioral changes induced by AVT might increase a male's attractiveness to females, and also may be consistent with an aggressive response to another túngara frog male. Copyright 2007 S. Karger AG, Basel.

  13. 76 FR 4896 - Call for Candidates

    Science.gov (United States)

    2011-01-27

    ... From the Federal Register Online via the Government Publishing Office FEDERAL ACCOUNTING STANDARDS ADVISORY BOARD Call for Candidates AGENCY: Federal Accounting Standards Advisory Board. ACTION: Notice... Federal Accounting Standards Advisory Board (FASAB) is currently seeking candidates (candidates must...

  14. Mourning Dove Call-count Survey

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Mourning Dove (Zenaida macroura) Call-Count Survey was developed to provide an index to population size and to detect annual changes in mourning dove breeding...

  15. Computer Assisted Language Learning (CALL) Software: Evaluation ...

    African Journals Online (AJOL)

    Computer Assisted Language Learning (CALL) Software: Evaluation of its Influence in a Language Learning Process. ... Current Issue · Archives · Journal Home > Vol 12, No 1 (2011) >. Log in or Register to get access to full text downloads.

  16. The function of migratory bird calls

    DEFF Research Database (Denmark)

    Reichl, Thomas; Andersen, Bent Bach; Larsen, Ole Næsbye

    The function of migratory bird calls: do they influence orientation and navigation?   Thomas Reichl1, Bent Bach Andersen2, Ole Naesbye Larsen2, Henrik Mouritsen1   1Institute of Biology, University of Oldenburg, Oldenburg, D-26111 Oldenburg, Germany 2Institute of Biology, University of Southern...... migration and to stimulate migratory restlessness in conspecifics. We wished to test if conspecific flight calls influence the flight direction of a nocturnal migrant, the European Robin (Erithacus rubecula), i.e. if flight calls help migrants keeping course. Wild caught birds showing migratory restlessness...... the experimental bird could be activated successively to simulate a migrating Robin cruising E-W, W-E, S-N or N-S at a chosen height (mostly about 40 m), at 10 m/s and emitting Robin flight calls of 80 dB(A) at 1 m. The simulated flight of a "ding" sound served as a control. During an experiment the bird was first...

  17. Partition Decomposition for Roll Call Data

    CERN Document Server

    Leibon, Greg; Rockmore, Daniel N; Savell, Robert

    2011-01-01

    In this paper we bring to bear some new tools from statistical learning on the analysis of roll call data. We present a new data-driven model for roll call voting that is geometric in nature. We construct the model by adapting the "Partition Decoupling Method," an unsupervised learning technique originally developed for the analysis of families of time series, to produce a multiscale geometric description of a weighted network associated to a set of roll call votes. Central to this approach is the quantitative notion of a "motivation," a cluster-based and learned basis element that serves as a building block in the representation of roll call data. Motivations enable the formulation of a quantitative description of ideology and their data-dependent nature makes possible a quantitative analysis of the evolution of ideological factors. This approach is generally applicable to roll call data and we apply it in particular to the historical roll call voting of the U.S. House and Senate. This methodology provides a...

  18. Communication cliques in mobile phone calling networks

    Science.gov (United States)

    Li, Ming-Xia; Xie, Wen-Jie; Jiang, Zhi-Qiang; Zhou, Wei-Xing

    2015-11-01

    People in modern societies form different social networks through numerous means of communication. These communication networks reflect different aspects of human's societal structure. The billing records of calls among mobile phone users enable us to construct a directed calling network (DCN) and its Bonferroni network (SVDCN) in which the preferential communications are statistically validated. Here we perform a comparative investigation of the cliques of the original DCN and its SVDCN constructed from the calling records of more than nine million individuals in Shanghai over a period of 110 days. We find that the statistical properties of the cliques of the two calling networks are qualitatively similar and the clique members in the DCN and the SVDCN exhibit idiosyncratic behaviors quantitatively. Members in large cliques are found to be spatially close to each other. Based on the clique degree profile of each mobile phone user, the most active users in the two calling networks can be classified in to several groups. The users in different groups are found to have different calling behaviors. Our study unveils interesting communication behaviors among mobile phone users that are densely connected to each other.

  19. 47 CFR 22.921 - 911 call processing procedures; 911-only calling mode.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false 911 call processing procedures; 911-only calling mode. 22.921 Section 22.921 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES PUBLIC MOBILE SERVICES Cellular Radiotelephone Service § 22.921 911 call...

  20. Pregnanes and other constituents of the roots of Macrosiphonia petraea (A. St.-Hil.) Kuntze (Apocynaceae); Pregnanos e outros constituintes das raizes de Macrosiphonia petraea (A. St.-Hil.) Kuntze (Apocynaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Assis Junior, Luiz Roberto de; Garcez, Fernanda Rodrigues; Garcez, Walmir Silva, E-mail: walmir.garcez@ufms.br [Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil). Centro de Ciencias Exatas e Tecnologia; Guterres, Zaira da Rosa [Universidade Estadual de Mato Grosso do Sul (UFMS), Mundo Novo, MS (Brazil)

    2013-09-01

    Macrosiphonia petraea (A. St.-Hil.) Kuntze is a plant popularly known as 'velame'. Its root infusion is used in the folk medicine of Mato Grosso do Sul, Brazil, for the treatment of inflammatory diseases. Phytochemical investigation of the roots of this species led to the identification of 17 compounds belonging to four different classes: two pregnanes, 12{beta}-hydroxypregna-4,6,16-triene-3,20-dione, neridienone A, and 12{beta}-hydroxypregna-4,6-diene-3,20-dione, cybisterol, one hydroxylated fatty acid, 5-hydroxy-octadeca-6(E)-8(Z)-dienoic acid, two lignoids, pinoresinol and 8{alpha}-hydroxypinoresinol, ten pentacyclic triterpenoids, and two steroids. (author)

  1. Calling patterns in human communication dynamics

    CERN Document Server

    Jiang, Zhi-Qiang; Li, Ming-Xia; Podobnik, Boris; Zhou, Wei-Xing; Stanley, H Eugene; 10.1073/pnas.1220433110

    2013-01-01

    Modern technologies not only provide a variety of communication modes, e.g., texting, cellphone conversation, and online instant messaging, but they also provide detailed electronic traces of these communications between individuals. These electronic traces indicate that the interactions occur in temporal bursts. Here, we study the inter-call durations of the 100,000 most-active cellphone users of a Chinese mobile phone operator. We confirm that the inter-call durations follow a power-law distribution with an exponential cutoff at the population level but find differences when focusing on individual users. We apply statistical tests at the individual level and find that the inter-call durations follow a power-law distribution for only 3460 individuals (3.46%). The inter-call durations for the majority (73.34%) follow a Weibull distribution. We quantify individual users using three measures: out-degree, percentage of outgoing calls, and communication diversity. We find that the cellphone users with a power-law...

  2. Dynamic routing based on call quality

    CERN Document Server

    Hammami, Oussama; Gabrielyan, Emin

    2009-01-01

    The telephony over IP (ToIP) is becoming a new trend in technology widely used nowadays in almost all business sectors. Its concepts rely on transiting the telephone communications through the IP network. Today, this technology is deployed increasingly what the cause of emergence of companies is offering this service as Switzernet. For several highly demanded destinations, recently fake vendors appeared in the market offering voice termination but providing only false answer supervision. The answered signal is returned immediately and calls are being charged without being connected. Different techniques are used to keep the calling party on the line. One of these techniques is to play a record of a ring back tone (while the call is already being charged). Another, more sophisticated technique is to play a human voice randomly picked up from a set of records containing contents similar to: hello, hello, I cannot hear you Apart the fact that the fallaciously established calls are charged at rates of real calls,...

  3. Steroids excreted in urine by neonates with 21-hydroxylase deficiency. 2. Characterization, using GC-MS and GC-MS/MS, of pregnanes and pregnenes with an oxo- group on the A- or B-ring.

    Science.gov (United States)

    Christakoudi, Sofia; Cowan, David A; Taylor, Norman F

    2012-04-01

    Urine from neonates with 21-hydroxylase deficiency contains a large range of metabolites of 17-hydroxyprogesterone, 21-deoxycortisol and androgens but few have been previously described. We present the second part of a comprehensive project to characterize and identify these in order to enhance diagnosis and to further elucidate neonatal steroid metabolism. Steroids were analyzed, after extraction and enzymatic conjugate hydrolysis, as methyloxime-trimethylsilyl ether derivatives on gas-chromatographs coupled to quadrupole and ion-trap mass-spectrometers. GC-MS and GC-MS/MS spectra were used together to determine the structure of the A- and B-rings containing an oxo group. Fragmentations indicating presence of 3-, 6-, and 7-oxo groups and also 1β-, 2α-, 4β-, and 6β-hydroxyls are presented and discussed for the first time. Interpretation was aided by comparison with spectra of available relevant standards, of oxidation products of standards and urinary metabolites and of deuterated derivatives. Endogenous 1-enes and 2(3)-ene artifacts of non-hydrolyzed 3α-sulfates are also reported. D-ring and side chain structure was determined according to our previously published criteria. Likely metabolic relationships were also explored. We conclude that GC-MS combined with GC-MS/MS allows identification of the A- and B-ring structure of pregnane and pregnenes in the presence of an oxo group on one of these rings. Major oxygenations are 1β, 15β, 16α and 21-hydroxy and 6- and 7-oxo groups. Minor positions of hydroxylation are those at 2α, 4β and 6β. Three major metabolic streams exist in affected neonates in addition to the classical 3α-hydroxy-5β-pregnane pathway, i.e. these of the 3-oxo-4-enes as well as 3α- and 3β-hydroxy-5α-anes.

  4. Regulation of pregnane-X-receptor, CYP3A and P-glycoprotein genes in the PCB-resistant killifish (Fundulus heteroclitus) population from New Bedford Harbor.

    Science.gov (United States)

    Gräns, Johanna; Wassmur, Britt; Fernández-Santoscoy, María; Zanette, Juliano; Woodin, Bruce R; Karchner, Sibel I; Nacci, Diane E; Champlin, Denise; Jayaraman, Saro; Hahn, Mark E; Stegeman, John J; Celander, Malin C

    2015-02-01

    Killifish survive and reproduce in the New Bedford Harbor (NBH) in Massachusetts (MA), USA, a site severely contaminated with polychlorinated biphenyls (PCBs) for decades. Levels of 22 different PCB congeners were analyzed in liver from killifish collected in 2008. Concentrations of dioxin-like PCBs in liver of NBH killifish were ∼400 times higher, and the levels of non-dioxin-like PCBs ∼3000 times higher than in killifish from a reference site, Scorton Creek (SC), MA. The NBH killifish are known to be resistant to the toxicity of dioxin-like compounds and to have a reduced aryl hydrocarbon receptor (AhR) signaling response. Little is known about the responses of these fish to non-dioxin-like PCBs, which are at extraordinarily high levels in NBH fish. In mammals, some non-dioxin-like PCB congeners act through nuclear receptor 1I2, the pregnane-X-receptor (PXR). To explore this pathway in killifish, a PXR cDNA was sequenced and its molecular phylogenetic relationship to other vertebrate PXRs was determined. Killifish were also collected in 2009 from NBH and SC, and after four months in the laboratory they were injected with a single dose of either the dioxin-like PCB 126 (an AhR agonist) or the non-dioxin-like PCB 153 (a mammalian PXR agonist). Gills and liver were sampled three days after injection and transcript levels of genes encoding PXR, cytochrome P450 3A (CYP3A), P-glycoprotein (Pgp), AhR2 and cytochrome P450 1A (CYP1A) were measured by quantitative PCR. As expected, there was little effect of PCB exposure on mRNA expression of AhR2 or CYP1A in liver and gills of NBH fish. In NBH fish, but not in SC fish, there was increased mRNA expression of hepatic PXR, CYP3A and Pgp upon exposure to either of the two PCB congeners. However, basal PXR and Pgp mRNA levels in liver of NBH fish were significantly lower than in SC fish. A different pattern was seen in gills, where there were no differences in basal mRNA expression of these genes between the two

  5. Regulation of pregnane-X-receptor, CYP3A and P-glycoprotein genes in the PCB-resistant killifish (Fundulus heteroclitus) population from New Bedford Harbor

    Energy Technology Data Exchange (ETDEWEB)

    Gräns, Johanna; Wassmur, Britt; Fernández-Santoscoy, María [Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE 405 30 Gothenburg (Sweden); Zanette, Juliano; Woodin, Bruce R.; Karchner, Sibel I. [Biology Department, MS #32, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Nacci, Diane E.; Champlin, Denise; Jayaraman, Saro [Office of Research and Development, National Health and Environmental Effects Research Laboratory, Atlantic Ecology Division, United States Environmental Protection Agency, 27 Tarzwell Drive, Narragansett, RI 02882 (United States); Hahn, Mark E.; Stegeman, John J. [Biology Department, MS #32, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Celander, Malin C., E-mail: malin.celander@gu.se [Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE 405 30 Gothenburg (Sweden)

    2015-02-15

    Highlights: • Basal levels of PXR and Pgp mRNA are lower in liver of fish from NBH than from SC. • Hepatic PXR, CYP3A and Pgp mRNA levels are induced by PCB in fish from NBH. • Both non-dioxin-like and dioxin-like PCBs induce PXR, CYP3A and Pgp in NBH fish. • Branchial PXR and CYP3A mRNA levels are induced by PCB 126 in fish from SC. • There is possible cross-talk between AhR and PXR signaling in killifish. - Abstract: Killifish survive and reproduce in the New Bedford Harbor (NBH) in Massachusetts (MA), USA, a site severely contaminated with polychlorinated biphenyls (PCBs) for decades. Levels of 22 different PCB congeners were analyzed in liver from killifish collected in 2008. Concentrations of dioxin-like PCBs in liver of NBH killifish were ∼400 times higher, and the levels of non-dioxin-like PCBs ∼3000 times higher than in killifish from a reference site, Scorton Creek (SC), MA. The NBH killifish are known to be resistant to the toxicity of dioxin-like compounds and to have a reduced aryl hydrocarbon receptor (AhR) signaling response. Little is known about the responses of these fish to non-dioxin-like PCBs, which are at extraordinarily high levels in NBH fish. In mammals, some non-dioxin-like PCB congeners act through nuclear receptor 1I2, the pregnane-X-receptor (PXR). To explore this pathway in killifish, a PXR cDNA was sequenced and its molecular phylogenetic relationship to other vertebrate PXRs was determined. Killifish were also collected in 2009 from NBH and SC, and after four months in the laboratory they were injected with a single dose of either the dioxin-like PCB 126 (an AhR agonist) or the non-dioxin-like PCB 153 (a mammalian PXR agonist). Gills and liver were sampled three days after injection and transcript levels of genes encoding PXR, cytochrome P450 3A (CYP3A), P-glycoprotein (Pgp), AhR2 and cytochrome P450 1A (CYP1A) were measured by quantitative PCR. As expected, there was little effect of PCB exposure on mRNA expression of

  6. Interactions of pharmaceuticals and other xenobiotics on hepatic pregnane X receptor and cytochrome P450 3A signaling pathway in rainbow trout (Oncorhynchus mykiss)

    Energy Technology Data Exchange (ETDEWEB)

    Wassmur, Britt; Graens, Johanna; Kling, Peter [University of Gothenburg, Department of Zoology, Box 463, SE-405 30 Goeteborg (Sweden); Celander, Malin C., E-mail: malin.celander@zool.gu.se [University of Gothenburg, Department of Zoology, Box 463, SE-405 30 Goeteborg (Sweden)

    2010-10-01

    The pregnane X receptor (PXR) belongs to the nuclear hormone receptor (NR) superfamily and is commonly described as a xenophore or a pharmacophore, as it can be activated by a wide array of xenobiotics, including numerous pharmaceuticals and other environmental pollutants. The PXR regulates expression of e.g. cytochrome P450 3A (CYP3A) and the P-glycoprotein (P-gp) that are involved in excretion of lipophilic xenobiotics and endobiotics. A full length PXR cDNA was isolated from rainbow trout liver and it was expressed in a descending order of magnitude in liver > intestine > kidney > heart. A rainbow trout PXR reporter assay was developed and a suite of pharmaceuticals and other xenobiotics were screened. However, no specific activation of rainbow trout PXR was observed with the substances tested. Interactions of prototypical PXR agonists on PXR signaling in rainbow trout were further investigated in cells of hepatic origin exposed in vitro and in juvenile rainbow trout exposed in vivo. The rainbow trout hepatoma cell line (RTH-149), displayed 600 times lower expression of CYP3A mRNA compared to primary cultures of hepatocytes, and did not respond to treatment with either pregnenolone 16{alpha}-carbonitrile (PCN), ketoconazole (KCZ) or rifampicin (RIF), which implies a non-functional PXR in this cell line. Exposure of hepatocytes to PCN and lithocholic acid (LA), resulted in a weak concentration-dependent induction of CYP3A and P-gp mRNA levels, though, exposure to the higher concentration of LA (50 {mu}M) decreased PXR mRNA levels. Exposure to dexamethasone (DEX) resulted in a decrease in PXR mRNA, without affecting CYP3A mRNA levels in hepatocytes in vitro. Injections of rainbow trout in vivo with 1 mg LA/kg fish resulted in a slight (albeit not significant) increase in CYP3A mRNA levels without affecting PXR mRNA levels. Although, injection with 10 mg omeprazole (OME)/kg fish had no effect on PXR and CYP3A mRNA levels, a 60% inhibition of CYP3A enzyme activities

  7. Dispatching function calls across accelerator devices

    Energy Technology Data Exchange (ETDEWEB)

    Jacob, Arpith C.; Sallenave, Olivier H.

    2017-01-17

    In one embodiment, a computer-implemented method for dispatching a function call includes receiving, at a supervisor processing element (PE) and from an origin PE, an identifier of a target device, a stack frame of the origin PE, and an address of a function called from the origin PE. The supervisor PE allocates a target PE of the target device. The supervisor PE copies the stack frame of the origin PE to a new stack frame on a call stack of the target PE. The supervisor PE instructs the target PE to execute the function. The supervisor PE receives a notification that execution of the function is complete. The supervisor PE copies the stack frame of the target PE to the stack frame of the origin PE. The supervisor PE releases the target PE of the target device. The supervisor PE instructs the origin PE to resume execution of the program.

  8. Malware Classification based on Call Graph Clustering

    CERN Document Server

    Kinable, Joris

    2010-01-01

    Each day, anti-virus companies receive tens of thousands samples of potentially harmful executables. Many of the malicious samples are variations of previously encountered malware, created by their authors to evade pattern-based detection. Dealing with these large amounts of data requires robust, automatic detection approaches. This paper studies malware classification based on call graph clustering. By representing malware samples as call graphs, it is possible to abstract certain variations away, and enable the detection of structural similarities between samples. The ability to cluster similar samples together will make more generic detection techniques possible, thereby targeting the commonalities of the samples within a cluster. To compare call graphs mutually, we compute pairwise graph similarity scores via graph matchings which approximately minimize the graph edit distance. Next, to facilitate the discovery of similar malware samples, we employ several clustering algorithms, including k-medoids and DB...

  9. Dispatching function calls across accelerator devices

    Energy Technology Data Exchange (ETDEWEB)

    Jacob, Arpith C.; Sallenave, Olivier H.

    2017-01-10

    In one embodiment, a computer-implemented method for dispatching a function call includes receiving, at a supervisor processing element (PE) and from an origin PE, an identifier of a target device, a stack frame of the origin PE, and an address of a function called from the origin PE. The supervisor PE allocates a target PE of the target device. The supervisor PE copies the stack frame of the origin PE to a new stack frame on a call stack of the target PE. The supervisor PE instructs the target PE to execute the function. The supervisor PE receives a notification that execution of the function is complete. The supervisor PE copies the stack frame of the target PE to the stack frame of the origin PE. The supervisor PE releases the target PE of the target device. The supervisor PE instructs the origin PE to resume execution of the program.

  10. Hourly associations between heat and ambulance calls.

    Science.gov (United States)

    Guo, Yuming

    2017-01-01

    The response speed of ambulance calls is very crucial to rescue patients suffering immediately life threatening conditions. The serious health outcomes might be caused by exposing to extreme heat only several hours before. However, limited evidence is available on this topic. This study aims to examine the hourly association between heat and ambulance calls, to improve the ambulance services and to better protect health. Hourly data on ambulance calls for non-accidental causes, temperature and air pollutants (PM10, NO2, and O3) were collected from Brisbane, Australia, during 2001 and 2007. A time-stratified case-crossover design was used to examine the associations between hourly ambulance calls and temperature during warm season (Nov, Dec, Jan, Feb, and Mar), while adjusting for potential confounders. Stratified analyses were performed for sex and age groups. Ambulance calls peaked at 10am for all groups, except those aged heat-ambulance calls relationships were non-linear for all groups, with thresholds between 27 °C and 31 °C. The associations appeared immediately, and lasted for about 24 h. There were no significant modification effect by sex and age. The findings suggest that hot hourly temperatures (>27 °C) increase the demands of ambulance. This information is helpful to increase the efficiency of ambulance service then save lives, for example, preparing more ambulance before appearance of extremely hot temperature in combination with weather forecast. Also, people should better arrange their time for outdoor activities to avoid exposing to extreme hot temperatures. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Analysis of The Call of The Wild

    Institute of Scientific and Technical Information of China (English)

    于婷婷

    2012-01-01

    The Call of The Wild is the most famous novel written by Jack London. In this novel, London depicts the story of a powerful dog called Buck, which happened in the Gold Rush Times when a great number of people came to Alaska to find gold. It tells us how Buck learns to struggle and strive, and how his wildness is aroused. By narrating Buck' s experience, London shows us some of the temporary social characters: the indispensable struggle for mastery and the nature of humanbeings -- wild etc. It reflects the major thoughts of that time. This essay will analyze this theme -- the indispensable struggle for mastery.

  12. Securing Remote Procedure Calls over HTTPS

    CERN Document Server

    Kale, Ajinkya; Pradhan, Sudeep

    2009-01-01

    Remote Procedure Calls (RPC) are widely used over the Internet as they provide a simple and elegant way of interaction between the client and the server. This paper proposes a solution for securing the remote procedure calls (RPC) by tunneling it through HTTPS (Hypertext Transfer Protocol over Secure Socket Layer). RPC over HTTP actually uses the Secure Socket Layer (SSL) protocol as a transport for the traffic. SSL mandates that the server authenticates itself to the client using a digital certificate (and associated private key). SSL is normally configured to encrypt traffic before transmitting it between the server and client and vice versa.

  13. ParticleCall: A particle filter for base calling in next-generation sequencing systems

    Directory of Open Access Journals (Sweden)

    Shen Xiaohu

    2012-07-01

    Full Text Available Abstract Background Next-generation sequencing systems are capable of rapid and cost-effective DNA sequencing, thus enabling routine sequencing tasks and taking us one step closer to personalized medicine. Accuracy and lengths of their reads, however, are yet to surpass those provided by the conventional Sanger sequencing method. This motivates the search for computationally efficient algorithms capable of reliable and accurate detection of the order of nucleotides in short DNA fragments from the acquired data. Results In this paper, we consider Illumina’s sequencing-by-synthesis platform which relies on reversible terminator chemistry and describe the acquired signal by reformulating its mathematical model as a Hidden Markov Model. Relying on this model and sequential Monte Carlo methods, we develop a parameter estimation and base calling scheme called ParticleCall. ParticleCall is tested on a data set obtained by sequencing phiX174 bacteriophage using Illumina’s Genome Analyzer II. The results show that the developed base calling scheme is significantly more computationally efficient than the best performing unsupervised method currently available, while achieving the same accuracy. Conclusions The proposed ParticleCall provides more accurate calls than the Illumina’s base calling algorithm, Bustard. At the same time, ParticleCall is significantly more computationally efficient than other recent schemes with similar performance, rendering it more feasible for high-throughput sequencing data analysis. Improvement of base calling accuracy will have immediate beneficial effects on the performance of downstream applications such as SNP and genotype calling. ParticleCall is freely available at https://sourceforge.net/projects/particlecall.

  14. 76 FR 36130 - Call for Candidates

    Science.gov (United States)

    2011-06-21

    ... From the Federal Register Online via the Government Publishing Office FEDERAL ACCOUNTING STANDARDS ADVISORY BOARD Call for Candidates AGENCY: Federal Accounting Standards Advisory Board. ACTION: Request for... Accounting Standards Advisory Board (FASAB or the Board) with the requested materials in response to...

  15. Authenticity in CALL: Three Domains of "Realness"

    Science.gov (United States)

    Buendgens-Kosten, Judith

    2013-01-01

    This paper discusses the role of authenticity and authenticity claims in computer assisted language learning (CALL). It considers authenticity as the result of a social negotiation process rather than an innate feature of a text, object, person, or activity. From this basis, it argues that authenticity claims play an important role in both second…

  16. A Call for School Counseling Practitioner Research

    Science.gov (United States)

    Kaffenberger, Carol J.

    2012-01-01

    Despite the repeated call to increase the number of practitioner research manuscripts being published in counseling publications, practitioner research accounts for less than five percent of all manuscripts published. This article describes the challenges faced by practitioners seeking to publish their research, defines practitioner research, and…

  17. A Simple Selection System Called "Jobmatch."

    Science.gov (United States)

    Sheibar, Paul

    1979-01-01

    An automated candidate-job matching system called "Jobmatch" has been implemented at Citibank, in New York City, involving job analysis, performance appraisal techniques, clerical employment, and internal mobility. A job task scale is used by supervisor and employee to determine job and candidate matching through a series of computer…

  18. When to call a linear system nonnegative

    NARCIS (Netherlands)

    Nieuwenhuis, J.W.

    1998-01-01

    In this paper we will consider discrete time invariant linear systems that allow for an input-state-output representation with a finite dimensional state space, and that have a finite number of inputs and outputs. The basic issue in this paper is when to call these systems nonnegative. An important

  19. Calling, is there anything special about it?

    African Journals Online (AJOL)

    2016-07-15

    Jul 15, 2016 ... While we admit that it is a core concept in theology and ministerial practice, we ask, ... It was this commitment to his calling that inspired us to explore the theme of ..... 'vocation', ended on a very personal note, emphasising the.

  20. Bridging CALL & HCI: Input from Participatory Design

    Science.gov (United States)

    Cardenas-Claros, Monica S.; Gruba, Paul A.

    2010-01-01

    Participatory design (PD), or the collaboration between software engineers and end users throughout the design process, may help improve CALL design practices. In this case study, four ESL learners, a software designer, and a language teacher created and evaluated a series of paper prototypes concerning help options in computer-based second…

  1. Bridging CALL & HCI: Input from Participatory Design

    Science.gov (United States)

    Cardenas-Claros, Monica S.; Gruba, Paul A.

    2010-01-01

    Participatory design (PD), or the collaboration between software engineers and end users throughout the design process, may help improve CALL design practices. In this case study, four ESL learners, a software designer, and a language teacher created and evaluated a series of paper prototypes concerning help options in computer-based second…

  2. Optimization of Overflow Policies in Call Centers

    DEFF Research Database (Denmark)

    Koole, G.M.; Nielsen, B.F.; Nielsen, T.B.

    2015-01-01

    We examine how overflow policies in a multi-skill call center should be designed to accommodate performance measures that depend on waiting time percentiles such as service level. This is done using a discrete Markovian approximation of the waiting time of the first customers waiting in line...... and customers are treated in a FCFS order....

  3. What Is This Thing Called Learner's Lexicography?

    DEFF Research Database (Denmark)

    Nielsen, Sandro

    2010-01-01

    Learner lexicography as a research area has attracted increased attention during the past decades, but what is actually the true nature of learner lexicography? This question calls for a complex answer. Learner lexicography has as its objective to develop principles that help practitioners to pro...

  4. Calling for Goddesses in Winesburg Ohio

    Institute of Scientific and Technical Information of China (English)

    付燕

    2013-01-01

    Winesburg Ohio, by Sherwood Anderson, depicts a group of people living in a transitional period where the human soci⁃ety was undergoing a sudden change from an agrarian society into a modern industrial one. This article will interpret Anderson’s call from the aspect of“Goddess Revival”.

  5. Make a 21st century phone call

    CERN Multimedia

    Katarina Anthony

    2014-01-01

    Want to avoid roaming charges? Click to call anyone at CERN? How about merging your CERN landline with your existing smartphone? That's all easily done with Lync, CERN's new opt-in service that can take your calls to the next level.   The Lync application on Windows (left) and iPhone (right). Lync unites CERN's traditional telephone service with the digital sphere. "Lync gives you the gift of mobility, by letting you access your CERN landline on the go," explains Pawel Grzywaczewski, service manager of the Lync system. "Once you've registered your CERN telephone with the service, you can run the Lync application and make calls from a range of supported devices. No matter where you are in the world - be it simply out to lunch or off at an international conference - you can make a CERN call as though you were in the office. All you need is an Internet connection!" Following a recent upgrade, CERN's Lync service now has...

  6. Time series modeling of daily abandoned calls in a call centre ...

    African Journals Online (AJOL)

    Time series modeling of daily abandoned calls in a call centre. ... were shown to be both parsimonious and adequate using the P-P plots, Q-Q plots and residual analysis. ... The data for application were got from a GSM telephone provider.

  7. BUSINESS MODELS FOR EXTENDING OF 112 EMERGENCY CALL CENTER CAPABILITIES WITH E-CALL FUNCTION INSERTION

    Directory of Open Access Journals (Sweden)

    Pop Dragos Paul

    2010-12-01

    Full Text Available The present article concerns present status of implementation in Romania and Europe of eCall service and the proposed business models regarding eCall function implementation in Romania. eCall system is used for reliable transmission in case of crush between In Vehicle System and Public Service Answering Point, via the voice channel of cellular and Public Switched Telephone Network (PSTN. eCall service could be initiated automatically or manual the driver. All data presented in this article are part of researches made by authors in the Sectorial Contract Implementation study regarding eCall system, having as partners ITS Romania and Electronic Solution, with the Romanian Ministry of Communication and Information Technology as beneficiary.

  8. Towards Building an Intelligent Call Routing System

    Directory of Open Access Journals (Sweden)

    Thien Khai Tran

    2016-01-01

    Full Text Available This paper presents EduICR - an Intelligent Call Routing system. This system can route calls to the most appropriate agent using routing rules built by the text classifier. EduICR includes the following main components: telephone communication network; Vietnamese speech recognition; Text classifier/ Natural language processor and Vietnamese speech synthesis. To our best knowledge, this is one of the first systems in Vietnam to implement the integration mechanism of text processing and speech processing. This allows voice applications to be more intelligent, able to communicate with humans in natural language with high accuracy and reasonable speed. Having been built and tested in real environment, our system proves its accuracy attaining more than 95%.

  9. Designing CALL Tasks for College English Learners

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    <正>As CALL(Computer Assisted Language Learning) became a new trend among China’s college English learners nowadays,it is time that teachers reflected on their teaching methodology innovation with the new technological advancement,one part of it is writing CALL tasks for their own individual classes.This paper presents a few basic principles and rationales of designing such tasks,and hope to work as a reminder for task writers.These principles and rationales include how to realize CLT(communicative language teaching) and learner-centredness and foster learner autonomy.In order to achieve this,it is reminded that task writers pay attention to a few points,like the communicativeness,contextualization,authenticity,and relatedness in task writing. Aspects of syllabus design,choice of texts,contents of activities and tasks and sequencing are put under investigation.

  10. Disentangling Social Networks inferred from Call Logs

    CERN Document Server

    Cebrian, Manuel; Kirkpatrick, Scott

    2010-01-01

    Analysis of an unusually detailed telephone call data set --- a month of nearly all mobile and landline phone calls placed during August 2005 the United Kingdom --- allows us to identify several different types of social networks that are formed, and relate them to different activities that generate them. We distinguish, among others, work-related and personal or leisure-focused activities and show that the networks they form have very different characteristics. Our principal tool for the analysis, k-core decomposition, shows that distinct distributions of connectivity are present in the two spheres, and that this differentiation affects dramatically the dynamics of information diffusion. Both differ from the simpler and more globally connected structure evident in communications data such as the Internet AS graph.

  11. What is that Thing Called Computer Science?

    Directory of Open Access Journals (Sweden)

    Nadya Schokosva

    2013-07-01

    Full Text Available XXI century society, called Knowledge Society, has a direct dependency of the software products, considered by many as the most important development of modern technology. This dependence generates the need of scientists and professionals who research and develop products that meet social demands. This article describes the computer science area as one of the most demanded professions in this reality, and in order to make it known to more people.

  12. Sleep Inertia and On-Call Readiness

    Science.gov (United States)

    2000-03-01

    effects of polyphasic & M.J. Colligan (Eds.), New York, and ultrashort sleep schedules. In: Why Spectrum, pp. 553-580. we nap, Evolution, Chronobiology...and Naitoh, P., Kelly, T., & Babkoff, H. (1993) Functions of Polyphasic and Ultrashort Sleep inertia, best time not to wake up? Sleep , C. Stampi Editor...UNCLASSIFIED Defense Technical Information Center Compilation Part Notice ADP010467 TITLE: Sleep Inertia and On-Call Readiness DISTRIBUTION: Approved

  13. Evaluating erroneous offside calls in soccer

    Science.gov (United States)

    Hüttermann, Stefanie; Noël, Benjamin; Memmert, Daniel

    2017-01-01

    The ability to simultaneously attend to multiple objects declines with increases in the visual angle separating distant objects. We explored whether these laboratory-measured limits on visual attentional spread generalize to a real life context: offside calls by soccer assistant referees. We coded all offside calls from a full year of first division German soccer matches. By determining the x-y coordinates of the relevant players and assistant referee on the soccer field we were able to calculate how far assistant referees had to spread their visual attention to perform well. Counterintuitively, assistant referees made fewer errors when they were farther away from the action due to an advantageous (smaller) visual angle on the game action. The pattern held even when we accounted for individual differences in a laboratory-based attentional spread measure of ten of the assistant referees. Our finding that errors are linked to smaller visual angles may explain the complaints of fans in some situations: Those seated directly behind the assistant referee, further from the players, might actually have it easier to make the right call because the relevant players would form a smaller visual angle. PMID:28333996

  14. “Computer Assisted Language Learning” (CALL

    Directory of Open Access Journals (Sweden)

    Nazlı Gündüz

    2005-10-01

    Full Text Available This article will provide an overview of computers; an overview of the history of CALL, itspros and cons, the internet, World Wide Web, Multimedia, and research related to the uses of computers in the language classroom. Also, it also aims to provide some background for the beginnerson using the Internet in language classes today. It discusses some of the common types of Internetactivities that are being used today, what the minimum requirements are for using the Internet forlanguage learning, and some easy activities you can adapt for your classes. Some special terminology related to computers will also be used in this paper. For example, computer assisted language learning(CALL refers to the sets of instructions which need to be loaded into the computer for it to be able to work in the language classroom. It should be borne in mind that CALL does not refer to the use of acomputer by a teacher to type out a worksheet or a class list or preparing his/her own teaching alone.Hardware refers to any computer equipment used, including the computer itself, the keyboard, screen (or the monitor, the disc-drive, and the printer. Software (computer programs refers to the sets of instructions which need to be loaded into the computer for it to be able to work.

  15. Evaluating erroneous offside calls in soccer.

    Science.gov (United States)

    Hüttermann, Stefanie; Noël, Benjamin; Memmert, Daniel

    2017-01-01

    The ability to simultaneously attend to multiple objects declines with increases in the visual angle separating distant objects. We explored whether these laboratory-measured limits on visual attentional spread generalize to a real life context: offside calls by soccer assistant referees. We coded all offside calls from a full year of first division German soccer matches. By determining the x-y coordinates of the relevant players and assistant referee on the soccer field we were able to calculate how far assistant referees had to spread their visual attention to perform well. Counterintuitively, assistant referees made fewer errors when they were farther away from the action due to an advantageous (smaller) visual angle on the game action. The pattern held even when we accounted for individual differences in a laboratory-based attentional spread measure of ten of the assistant referees. Our finding that errors are linked to smaller visual angles may explain the complaints of fans in some situations: Those seated directly behind the assistant referee, further from the players, might actually have it easier to make the right call because the relevant players would form a smaller visual angle.

  16. Short-chain (C{sub 21} and C{sub 22}) diasteranes in petroleum and source rocks as indicators of maturity and depositional environment

    Energy Technology Data Exchange (ETDEWEB)

    Requejo, A.G. [Texas A & M Univ., College Station, TX (United States)]|[Exxon Production Research Co., Houston, TX (United States); McDonald, T.J.; Sassen, R. [Texas A & M Univ., College Station, TX (United States)] [and others

    1997-07-01

    Geochemical analysis of steranes in source rocks from the Western Canada Basin has allowed the identification of several C{sub 21} and C{sub 22} short-chain sterane isomers. The principal compounds in carbonates from the U. Jurassic Nordegg Formation have been identified as diginane and homodiginane, more thermodynamically-stable isomers of the compounds pregnane and homopregnane that have 5{alpha}, 14{beta},17{beta}(H) stereochemistry. The Triassic Doig formation also contains two compounds tentatively identified on the basis of geologic and geochemical data as diapregnane and diahomopregnane, rearranged isomers of pregnane and homopregnane with methyl substitution at the 5- and 14-carbon positions. The distribution of these compounds parallels that of the higher molecular weight diasteranes. A well-defined relationship is evident between the ratio Fe/S and short-chain diasterane content: samples with Fe/S approximately <0.90 exhibit low diasterane contents, while those with Fe/S approximately >0.90 exhibit high diasterane content. This threshold Fe/S value is nearly equal to the value associated with stoichiometric pyrite (0.87) and suggests that the abundance of short-chain diasteranes is related to excess iron associated with detrital clays. This is consistent with the accepted mechanism of sterane rearrangement, which is thought to proceed via catalysis of clay minerals. In contrast to the higher molecular-weight diasteranes, which increase in abundance relative to other sterane isomers with increasing thermal maturation, the abundance of the rearranged short-chain compounds varies little relative to diginane or homodiginane in a maturity suite from the U. Devonian Duvernay Formation. 38 refs., 11 figs., 3 tabs.

  17. Short-chain (C 21 and C 22) diasteranes in petroleum and source rocks as indicators of maturity and depositional environment

    Science.gov (United States)

    Requejo, A. G.; Hieshima, G. B.; Hsu, C. S.; McDonald, T. J.; Sassen, R.

    1997-07-01

    Geochemical analysis of steranes in source rocks from the Western Canada Basin has allowed the identification of several C 21 and C 22 short-chain sterane isomers. The principal compounds in carbonates from the U. Jurassic Nordegg Formation have been identified as diginane and homodiginane, more thermodynamically-stable isomers of the compounds pregnane and homopregnane that have 5α,14β,17β(H) stereochemistry. The Triassic Doig formation also contains two compounds tentatively identified on the basis of geologic and geochemical data as diapregnane and diahomopregnane, rearranged isomers of pregnane and homopregnane with methyl substitution at the 5- and 14-carbon positions. The distribution of these compounds parallels that of the higher molecular weight diasteranes. A well-defined relationship is evident between the ratio Fe/S and short-chain diasterane content: samples with Fe/S approximately diasterane contents, while those with Fe/S approximately >0.90 exhibit high diasterane content. This threshold Fe/S value is nearly equal to the value associated with stoichiometric pyrite (0.87) and suggests that the abundance of short-chain diasteranes is related to excess iron associated with detrital clays. This is consistent with the accepted mechanism of sterane rearrangement, which is thought to proceed via catalysis of clay minerals. In contrast to the higher molecular-weight diasteranes, which increase in abundance relative to other sterane isomers with increasing thermal maturation, the abundance of the rearranged short-chain compounds varies little relative to diginane or homodiginane in a maturity suite from the U. Devonian Duvernay Formation. Predicted stabilities based on computational chemistry agree with the observed distributions; diginane/diapregnane and homodiginane/homodiapregnane represent compound pairs with similar thermal stabilities. The relative invariance of short-chain sterane distributions with maturity suggest that these compounds may be

  18. SNP calling by sequencing pooled samples

    Directory of Open Access Journals (Sweden)

    Raineri Emanuele

    2012-09-01

    Full Text Available Abstract Background Performing high throughput sequencing on samples pooled from different individuals is a strategy to characterize genetic variability at a small fraction of the cost required for individual sequencing. In certain circumstances some variability estimators have even lower variance than those obtained with individual sequencing. SNP calling and estimating the frequency of the minor allele from pooled samples, though, is a subtle exercise for at least three reasons. First, sequencing errors may have a much larger relevance than in individual SNP calling: while their impact in individual sequencing can be reduced by setting a restriction on a minimum number of reads per allele, this would have a strong and undesired effect in pools because it is unlikely that alleles at low frequency in the pool will be read many times. Second, the prior allele frequency for heterozygous sites in individuals is usually 0.5 (assuming one is not analyzing sequences coming from, e.g. cancer tissues, but this is not true in pools: in fact, under the standard neutral model, singletons (i.e. alleles of minimum frequency are the most common class of variants because P(f ∝ 1/f and they occur more often as the sample size increases. Third, an allele appearing only once in the reads from a pool does not necessarily correspond to a singleton in the set of individuals making up the pool, and vice versa, there can be more than one read – or, more likely, none – from a true singleton. Results To improve upon existing theory and software packages, we have developed a Bayesian approach for minor allele frequency (MAF computation and SNP calling in pools (and implemented it in a program called snape: the approach takes into account sequencing errors and allows users to choose different priors. We also set up a pipeline which can simulate the coalescence process giving rise to the SNPs, the pooling procedure and the sequencing. We used it to compare the

  19. Calling patterns in human communication dynamics.

    Science.gov (United States)

    Jiang, Zhi-Qiang; Xie, Wen-Jie; Li, Ming-Xia; Podobnik, Boris; Zhou, Wei-Xing; Stanley, H Eugene

    2013-01-29

    Modern technologies not only provide a variety of communication modes (e.g., texting, cell phone conversation, and online instant messaging), but also detailed electronic traces of these communications between individuals. These electronic traces indicate that the interactions occur in temporal bursts. Here, we study intercall duration of communications of the 100,000 most active cell phone users of a Chinese mobile phone operator. We confirm that the intercall durations follow a power-law distribution with an exponential cutoff at the population level but find differences when focusing on individual users. We apply statistical tests at the individual level and find that the intercall durations follow a power-law distribution for only 3,460 individuals (3.46%). The intercall durations for the majority (73.34%) follow a Weibull distribution. We quantify individual users using three measures: out-degree, percentage of outgoing calls, and communication diversity. We find that the cell phone users with a power-law duration distribution fall into three anomalous clusters: robot-based callers, telecom fraud, and telephone sales. This information is of interest to both academics and practitioners, mobile telecom operators in particular. In contrast, the individual users with a Weibull duration distribution form the fourth cluster of ordinary cell phone users. We also discover more information about the calling patterns of these four clusters (e.g., the probability that a user will call the c(r)-th most contact and the probability distribution of burst sizes). Our findings may enable a more detailed analysis of the huge body of data contained in the logs of massive users.

  20. Call for another special issue / book

    Science.gov (United States)

    Bostenaru Dan, M.

    2009-04-01

    We would like to continue the series of special issue or maybe edit a book on this topic. To complete the formerly edited special issues we would like to link natural hazards research to cultural heritage research. We see a way of doing this connected to "integrated conservation", which sees the involvment of urban planning in conservation, as well as the (urban) sociology, the integration of the user, the participatism. We further call for investigation of GIS applications for the investigation of natural hazards' impact in this field. We are open for further ideas and wait for you at the Splinter meeting.

  1. First Class Call Stacks: Exploring Head Reduction

    Directory of Open Access Journals (Sweden)

    Philip Johnson-Freyd

    2016-06-01

    Full Text Available Weak-head normalization is inconsistent with functional extensionality in the call-by-name λ-calculus. We explore this problem from a new angle via the conflict between extensionality and effects. Leveraging ideas from work on the λ-calculus with control, we derive and justify alternative operational semantics and a sequence of abstract machines for performing head reduction. Head reduction avoids the problems with weak-head reduction and extensionality, while our operational semantics and associated abstract machines show us how to retain weak-head reduction's ease of implementation.

  2. Call center. Centrados en el cliente

    OpenAIRE

    Leal-Alonso-de-Castañeda, José Enrique

    2003-01-01

    La empresa actual ha de estar preparada para responder al Cliente tal y como éste espera, porque no se busca un cliente puntual, sino un cliente fiel. La globalización de la economía y del acceso a los mercados exige que la empresa sea capaz de atraer al cliente no sólo con un servicio de calidad, sino además con una atención de calidad. La implantación de un Call Center (Centro de Atención al Cliente, Centro de Atención de Llamadas) constituye por todo ello una estrategia de negocio qu...

  3. [Emergency calls in the Samu system].

    Science.gov (United States)

    Barrier, G

    2001-07-01

    The French emergency medical system is public and medical. Named Samu, it is almost entirely managed by anesthesiologists specially trained for emergency medical assistance and advanced medical support. The system comprises a reception and dispatching center for emergency calls, directly connected to police and firemen, but assuring patient confidentiality. This fixed center is managing mobile units which are Medical Intensive Care Ambulances (MICA named Smur in France). The Samu's mission consists in performing urgent individual medical assistance and also medical services for treatment of mass casualties. It is also to find adequate hospital units for the patients, to perform medical transport and to be a consultant for general practitioners in emergency.

  4. Theology links Christian ministry with God's call.

    Science.gov (United States)

    O'Connell, L J

    1984-03-01

    Catholic health care ministry originates in and is shaped by the theme of call in the Old and New Testaments. To be specifically Catholic, health professionals and facilities must define their ministries according to the values expressed in this theological tradition. Sponsorship. The opportunity to provide health care enables religious communities to contribute to God's ongoing creation process and to reiterate Christ's call to minister to others. Although health care facility sponsorship thrusts religious communities into the arena of big business, the abandonment of the health care mission could be considered a betrayal of evangelical values. Quality of life. The implicit concern for human dignity that distinguishes Catholic health care facilities should be evident in personalized patient care, just working conditions, and a commitment to healing in the civic community. Stewardship in ethics. The development of business policies and procedures and institutional responses to social change should be carefully considered in light of the Catholic understanding of loving covenant and the Christian way of life. Shared ministry. Health care facilities have played a leading role in implementing the Second Vatican Council's vision of ministry. Sponsoring communities' continued willingness to share responsibilities with laity will be imperative in meeting the health care demands of the future.

  5. Detecting Motifs in System Call Sequences

    CERN Document Server

    Wilson, William O; Aickelin, Uwe

    2010-01-01

    The search for patterns or motifs in data represents an area of key interest to many researchers. In this paper we present the Motif Tracking Algorithm, a novel immune inspired pattern identification tool that is able to identify unknown motifs which repeat within time series data. The power of the algorithm is derived from its use of a small number of parameters with minimal assumptions. The algorithm searches from a completely neutral perspective that is independent of the data being analysed, and the underlying motifs. In this paper the motif tracking algorithm is applied to the search for patterns within sequences of low level system calls between the Linux kernel and the operating system's user space. The MTA is able to compress data found in large system call data sets to a limited number of motifs which summarise that data. The motifs provide a resource from which a profile of executed processes can be built. The potential for these profiles and new implications for security research are highlighted. A...

  6. Milton Nascimento: una calle llamada mundo

    Directory of Open Access Journals (Sweden)

    Alberto Carlos De Souza

    2011-08-01

    Full Text Available El punto de partida de este estudio consistirá en la exploración de la musicalidad de Milton Nascimento y de sus compañeros del “movimiento” “Clube da Esquina”, destacándose la relevancia del conjunto de esa obra para la cultura brasilera. El referido “movimiento” floreció en Minas Gerais en el auge de uno de los períodos más críticos de la historia contemporánea brasilera: la dictadura militar. En tanto, el “Clube da Esquina” – dado a la diversidad de los temas tratados en sus letras y su singularidad poética -, luego se difundió por todo el espacio cultural brasilero. Hablar del “Clube da Esquina” no es tarea fácil porque hay una dolorosa ausencia o pálida presencia de este movimiento en estudios que se ocupan de nuestra música popular. El “Clube da Esquina” fue un círculo de amigos que se reunieron en un pequeño pub en la esquina de la Calle Divinópolis y Calle Paraisópolis, en un bucólico barrio de Belo Horizonte (Minas Gerais - Brasil, llamado Santa Teresa. Era parte de esa hermandad, interesada en música, cine y poesía, Milton Nascimento, Wagner Tiso, Fernando Brant, Toninho Horta, Beto Guedes, Moura Tavinho, los hermanos Lô y Marcio Borges, Robertinho Silva, Nivaldo Ornelas, Ronaldo Bastos, Murilo Antunes Nelson Angelo y Novelli, entre otros. En estas reuniones, regadas con mucha cerveza, Milton Nascimento y sus compañeros. Milton fue y sigue siendo la mayor referencia del movimiento “Clube da Esquina”. Abstract The starting point of this study will explore the music of Milton Nascimento and his fellow "movement" "Clube da Esquina", highlighting the relevance of all this work to the Brazilian culture. The aforementioned "movement" flourished in Minas Gerais at the height of one of the most critical periods in Brazilian contemporary history: the military dictatorship. Meanwhile, the "Clube da Esquina" - given the diversity of topics in his lyrics and his unique poetic - and then spread

  7. Call research: major themes and issues Call research: major themes and issues

    Directory of Open Access Journals (Sweden)

    Mike Levy

    2008-04-01

    Full Text Available Ao invés de apresentar uma perspectiva subjetiva sobre temas principais e questões de pesquisa em CALL, este artigo pretende oferecer um relatório em que, pelo menos em parte, os conceitos desenvolvidos são extraídos a partir de evidências empíricas. Espera-se que esse enfoque venha a oferecer uma descrição mais objetiva do trabalho contemporâneo de pesquisa em CALL. Para alcançar este objetivo, o presente trabalho está baseado em um corpus de 47 artigos de pesquisas em CALL, publicados em livros e periódicos de 1999. Com este fundamento, apresenta-se um modelo para a descrição e análise das pesquisas em CALL, assim como são representadas na literatura. Dois principais direcionamentos e outros três importantes, porém menos freqüentes, são descritos em detalhes, usando exemplos do corpus; e também consideram-se as implicações para pesquisas futuras. Uma ênfase particular será dada à identificação das metas dos pesquisadores de CALL e ao esclarecimento dos atributos singulares da pesquisa neste campo. Rather than provide a purely subjective perspective on major themes and issues in CALL research, this paper aims to provide an account where, at least in part, the views advanced are drawn from empirical evidence. It is hoped that this approach will provide a more objective description of contemporary CALL research work. To achieve this goal, the present paper is based upon a corpus of 47 CALL research articles published in books and journals in 1999. With this foundation it sets out a framework for the description and analysis of CALL research as it is represented in the literature. Two major directions and three important, though less frequent, directions are described in detail, using examples from the corpus, and the implications for research in the future are considered. Particular emphasis will be placed on identifying the goals of CALL researchers and on clarifying the unique attributes of research in this

  8. Learner Attitudes towards CALL Applications at YADIM

    Directory of Open Access Journals (Sweden)

    Zuhal OKAN & Pınar TORUN

    2007-12-01

    Full Text Available The use of educational technology to assist learners in their language studies has become agreat concern for scholars over the past decade. YADIM (School for Foreign Languages at ÇukurovaUniversity in Turkey has invested substantially to introduce students to computer-based languagelearning materials and to integrate technology into existing curriculum. This paper reports on attitudesof YADİM students towards the use of computer-assisted language learning (CALL and theirperceptions on CALL’s relevancy to their regular classroom work. Surveys and follow-up interviewsexplored how learners see the role of the instructor and the accessibility of the labs. The paper alsolooks at how students assess the impact of technology on their language learning process.

  9. Comprometimento organizacional de trabalhadores de call center

    Directory of Open Access Journals (Sweden)

    Kely César Martins Paiva

    2015-09-01

    Full Text Available RESUMOO Neste artigo, analisa-se como se apresenta o comprometimento organizacional de trabalhadores de um call center, localizado em Belo (A Horizonte (Minas Gerais, Brasil. Após o delineamento conceitual UJ do tema central, são expostos os resultados de um estudo de caso descritivo, realizado com abordagens quantitativa e qualitativa. Os dados de 399 questionários e 22 entrevistas são, respectivamente, tratados estatisticamente e submetidos à análise de conteúdo. A base de comprometimento que predominou entre esses infoproletários foi "obrigação pelo desempenho" e, em menor grau, "afetiva". Foi observado que quanto maior é o seu tempo de experiência nesse tipo de organização, menores são os níveis de comprometimento de modo geral, fatos esclarecidos, parcialmente, por meio das entrevistas.

  10. Acoustic signal detection of manatee calls

    Science.gov (United States)

    Niezrecki, Christopher; Phillips, Richard; Meyer, Michael; Beusse, Diedrich O.

    2003-04-01

    The West Indian manatee (trichechus manatus latirostris) has become endangered partly because of a growing number of collisions with boats. A system to warn boaters of the presence of manatees, that can signal to boaters that manatees are present in the immediate vicinity, could potentially reduce these boat collisions. In order to identify the presence of manatees, acoustic methods are employed. Within this paper, three different detection algorithms are used to detect the calls of the West Indian manatee. The detection systems are tested in the laboratory using simulated manatee vocalizations from an audio compact disc. The detection method that provides the best overall performance is able to correctly identify ~=96% of the manatee vocalizations. However the system also results in a false positive rate of ~=16%. The results of this work may ultimately lead to the development of a manatee warning system that can warn boaters of the presence of manatees.

  11. Learner Attitudes towards CALL Applications at YADIM

    Directory of Open Access Journals (Sweden)

    Zuhal OKAN & Pınar TORUN

    2014-01-01

    Full Text Available The use of educational technology to assist learners in their language studies has become agreat concern for scholars over the past decade. YADIM (School for Foreign Languages at ÇukurovaUniversity in Turkey has invested substantially to introduce students to computer-based languagelearning materials and to integrate technology into existing curriculum. This paper reports on attitudesof YADİM students towards the use of computer-assisted language learning (CALL and theirperceptions on CALL’s relevancy to their regular classroom work. Surveys and follow-up interviewsexplored how learners see the role of the instructor and the accessibility of the labs. The paper alsolooks at how students assess the impact of technology on their language learning process.

  12. Sharing programming resources between Bio* projects through remote procedure call and native call stack strategies

    DEFF Research Database (Denmark)

    Prins, Pjotr; Goto, Naohisa; Yates, Andrew

    2012-01-01

    into R through native R, RSOAP, Rserve, and RPy interfaces, with the performance of native BioPerl, Biopython, BioJava, and BioRuby implementations, and with call stack bindings to BioJava and the European Molecular Biology Open Software Suite. In general, call stack approaches outperform native Bio......, and languages sharing the Java Virtual Machine stack. This functionality provides strategies for sharing of software between Bio* projects, which can be exploited more often. Here, we present cross-language examples for sequence translation, and measure throughput of the different options. We compare calling......* implementations and these, in turn, outperform RPC-based approaches. To test and compare strategies, we provide a downloadable BioNode image with all examples, tools, and libraries included. The BioNode image can be run on VirtualBox-supported operating systems, including Windows, OSX, and Linux....

  13. Sharing programming resources between Bio* projects through remote procedure call and native call stack strategies.

    Science.gov (United States)

    Prins, Pjotr; Goto, Naohisa; Yates, Andrew; Gautier, Laurent; Willis, Scooter; Fields, Christopher; Katayama, Toshiaki

    2012-01-01

    Open-source software (OSS) encourages computer programmers to reuse software components written by others. In evolutionary bioinformatics, OSS comes in a broad range of programming languages, including C/C++, Perl, Python, Ruby, Java, and R. To avoid writing the same functionality multiple times for different languages, it is possible to share components by bridging computer languages and Bio* projects, such as BioPerl, Biopython, BioRuby, BioJava, and R/Bioconductor. In this chapter, we compare the two principal approaches for sharing software between different programming languages: either by remote procedure call (RPC) or by sharing a local call stack. RPC provides a language-independent protocol over a network interface; examples are RSOAP and Rserve. The local call stack provides a between-language mapping not over the network interface, but directly in computer memory; examples are R bindings, RPy, and languages sharing the Java Virtual Machine stack. This functionality provides strategies for sharing of software between Bio* projects, which can be exploited more often. Here, we present cross-language examples for sequence translation, and measure throughput of the different options. We compare calling into R through native R, RSOAP, Rserve, and RPy interfaces, with the performance of native BioPerl, Biopython, BioJava, and BioRuby implementations, and with call stack bindings to BioJava and the European Molecular Biology Open Software Suite. In general, call stack approaches outperform native Bio* implementations and these, in turn, outperform RPC-based approaches. To test and compare strategies, we provide a downloadable BioNode image with all examples, tools, and libraries included. The BioNode image can be run on VirtualBox-supported operating systems, including Windows, OSX, and Linux.

  14. Calling under pressure: Short-finned pilot whales make social calls during deep foraging dives

    DEFF Research Database (Denmark)

    Jensen, Frants Havmand; Marrero Perez, Jacobo; Johnson, Mark

    2011-01-01

    Toothed whales rely on sound to echolocate prey and communicate with conspecifics, but little is known about how extreme pressure affects pneumatic sound production in deep-diving species with a limited air supply. The short-finned pilot whale (Globicephala macrorhynchus) is a highly social species...... that the energy content of calls is lower at depths where lungs are collapsed and where the air volume available for sound generation is limited by ambient pressure. Frequency content was unaffected, providing a possible cue for group or species identification of diving whales. Social calls may be important...... to maintain social ties for foraging animals, but may be impacted adversely by vessel noise....

  15. Calle y calles de Brasil: imágenes, lecturas y etnografías

    OpenAIRE

    Montoya-Uriarte, Urpi; Universidad Federal de Bahía (Brasil)

    2015-01-01

    En este artículo me propongo reflexionar acerca de la calle de la ciudad contemporánea, movida por una pasión por lo urbano y una formación en antropología. La vida de las calles nos dice mucho sobre el tipo de ciudad en las que se encuentran. Sin embargo, son pocos los esfuerzos para captarla en sus detalles, particularidades, singularidades. Como urbanistas, parecemos conformarnos con las imágenes mentales que tenemos de ellas y como intelectuales, muchas veces, nos contentamos con reproduc...

  16. Itraconazole cis-diastereoisomers activate aryl hydrocarbon receptor AhR and pregnane X receptor PXR and induce CYP1A1 in human cell lines and human hepatocytes.

    Science.gov (United States)

    Stepankova, Martina; Pastorkova, Barbora; Bachleda, Petr; Dvorak, Zdenek

    2017-04-05

    Triazole antimycotic itraconazole contains in its structure three chiral centres; therefore, it forms eight stereoisomers. Commercial preparations of itraconazole are a mixture of four cis-diastereoisomers. There is much evidence that efficacy, adverse effects, and toxicity of chiral drugs may be stereospecific. Therefore, we have prepared 4 pure cis-diastereoisomers of itraconazole and investigated their effects on transcriptional activities of xenoreceptors aryl hydrocarbon receptor AhR and pregnane X receptor PXR. Gene reporter assays showed that itraconazole dose-dependently activated both AhR and PXR, and the activation of AhR but not of PXR was enantiospecific. Itraconazole diastereoisomers transformed AhR and PXR into their DNA-binding forms, as demonstrated by electromobility shift assays. Cytochrome P450 CYP1A1 mRNA and protein were induced by itraconazole diastereoisomers in human hepatoma cells HepG2, human skin cells HaCaT, and in primary human hepatocytes. The expression of CYP3A4 in human intestinal LS180 cells was not influenced by itraconazole, but we observed downregulation of CYP3A4 in human hepatocytes. Collectively, we show that itraconazole is a dual activator of AhR and PXR, with differential effects on the target genes for xenoreceptors. The enantiospecific pattern was observed only in gene reporter assays for AhR. The data presented here might be of toxicological and clinical importance.

  17. CALL and Less Commonly Taught Languages: Challenges and Opportunities

    Science.gov (United States)

    Ward, Monica

    2015-01-01

    Computer-Assisted Language Learning (CALL) researchers face many challenges in developing effective, high-quality CALL. CALL research has a very strong focus on the Most Commonly Taught Languages (MCLTs), particularly English. CALL researchers working with Less Commonly Taught Languages (LCTLs) face further constraints. LCTLs can range from…

  18. 47 CFR 74.791 - Digital call signs.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Digital call signs. 74.791 Section 74.791... Booster Stations § 74.791 Digital call signs. (a) Digital low power stations. Call signs for digital low... −D. (b) Digital television translator stations. Call signs for digital television translator...

  19. From Systematic Review to Call for Action.

    Science.gov (United States)

    Sawin, Erika Metzler; Sobel, Linda L; Annan, Sandra L; Schminkey, Donna L

    2017-06-01

    Intimate partner violence (IPV) is a global public health and criminal justice concern with significant impacts; especially high rates are seen among rural Hispanic American (HA) communities, the fastest growing population in the United States. They experience additional barriers to care including extreme poverty, lesser education, gender norms, and language and immigration issues. A systematic literature review was conducted using Cooper's framework to identify evidence supporting associations between interventions and prevention, reduction, and elimination of IPV among rural HA women. Searches conducted on databases including CINAHL, PubMed, Medline, Women's Studies International, MedicLatina, and JSTOR used the MeSH terms Hispanic Americans (Latino/a and Hispanic), domestic violence, and intimate partner violence. Selected studies were published between January 1, 2000, and January 1, 2014. Of the 617 yielded articles, only 6 met the inclusion criteria. Of these, none closely examined rurality or provided valid and reliable measures of outcomes, instead reporting program descriptions and suggested interventions. We identify key findings to guide program, screening, and tool development. Our study identifies a gap in knowledge, research, and effective practices and issues a call for action to create evidence-based tools to prevent, reduce, and eliminate IPV in these underserved populations.

  20. Pathological aspects of so called "hilar cholangiocarcinoma"

    Science.gov (United States)

    Castellano-Megías, Víctor M; Ibarrola-de Andrés, Carolina; Colina-Ruizdelgado, Francisco

    2013-01-01

    Cholangiocarcinoma (CC) arising from the large intrahepatic bile ducts and extrahepatic hilar bile ducts share clinicopathological features and have been called hilar and perihilar CC as a group. However, “hilar and perihilar CC” are also used to refer exclusively to the intrahepatic hilar type CC or, more commonly, the extrahepatic hilar CC. Grossly, a major distinction can be made between papillary and non-papillary tumors. Histologically, most hilar CCs are well to moderately differentiated conventional type (biliary) carcinomas. Immunohistochemically, CK7, CK20, CEA and MUC1 are normally expressed, being MUC2 positive in less than 50% of cases. Two main premalignant lesions are known: biliary intraepithelial neoplasia (BilIN) and intraductal papillary neoplasm of the biliary tract (IPNB). IPNB includes the lesions previously named biliary papillomatosis and papillary carcinoma. A series of 29 resected hilar CC from our archives is reviewed. Most (82.8%) were conventional type adenocarcinomas, mostly well to moderately differentiated, although with a broad morphological spectrum; three cases exhibited a poorly differentiated cell component resembling signet ring cells. IPNB was observed in 5 (17.2%), four of them with an associated invasive carcinoma. A clear cell type carcinoma, an adenosquamous carcinoma and two gastric foveolar type carcinomas were observed. PMID:23919110

  1. Let's call it Nonlocal Quantum Physics

    CERN Document Server

    Requardt, M

    2000-01-01

    In the following we undertake to derive quantum theory as a stochastic low-energy and coarse-grained theory from a more primordial discrete and basically geometric theory living on the Planck scale and which (as we argue) possibly underlies also \\tit{string theory}. We isolate the so-called \\tit{ideal elements} which represent at the same time the cornerstones of the framework of ordinary quantum theory and show how and why they encode the \\tit{non-local} aspects, being ubiquituous in the quantum realm, in a, on the surface, local way. We show that the quantum non-locality emerges in our approach as a natural consequence of the underlying \\tit{two-storey} nature of space-time or the physical vacuum, that is, quantum theory turns out to be a residual effect of the geometric depth structure of space-time on the Planck scale. We indicate how the \\tit{measurement problem} and the emergence of the \\tit{macroscopic sub-regime} can be understood in this framework.

  2. REMINDER: In a medical emergency call 74444

    CERN Multimedia

    2005-01-01

    What happened? A CERN colleague, complaining of pains that might indicate serious heart problem, went to the ?infirmary' on the Prévessin site for medical aid. He was unaware that the ?infirmary' was in fact no such thing, but the office of the French contractors' medical practitioner, and, on top of that, it was closed. He therefore took his own car and went to the CERN Fire Station on the Meyrin Site (Building 65). The firemen and the CERN medical team took care of him and requested helicopter transport to the Geneva cantonal hospital, where he responded well to medical treatment. What do we learn from this event? You must call the CERN internal number 74444 in the event of serious and acute illness, and do not have to present yourself in person or get somebody to go with you. This number is not reserved exclusively for accident, pollution, fire etc. The Firemen can prodice professional assistance at all times as required: first aid on the spot, amulance transport and medical assistance as necessary. ...

  3. Mitigating Handoff Call Dropping in Wireless Cellular Networks: A Call Admission Control Technique

    Science.gov (United States)

    Ekpenyong, Moses Effiong; Udoh, Victoria Idia; Bassey, Udoma James

    2016-06-01

    Handoff management has been an important but challenging issue in the field of wireless communication. It seeks to maintain seamless connectivity of mobile users changing their points of attachment from one base station to another. This paper derives a call admission control model and establishes an optimal step-size coefficient (k) that regulates the admission probability of handoff calls. An operational CDMA network carrier was investigated through the analysis of empirical data collected over a period of 1 month, to verify the performance of the network. Our findings revealed that approximately 23 % of calls in the existing system were lost, while 40 % of the calls (on the average) were successfully admitted. A simulation of the proposed model was then carried out under ideal network conditions to study the relationship between the various network parameters and validate our claim. Simulation results showed that increasing the step-size coefficient degrades the network performance. Even at optimum step-size (k), the network could still be compromised in the presence of severe network crises, but our model was able to recover from these problems and still functions normally.

  4. Glucocorticoid and androgen signaling pathways diverge between advertisement calling and non-calling fish.

    Science.gov (United States)

    Genova, Rachel M; Marchaterre, Margaret A; Knapp, Rosemary; Fergus, Daniel; Bass, Andrew H

    2012-09-01

    Behavioral and neuroendocrine mechanisms of social vocalization in teleost fish are influenced by the glucocorticoid cortisol and the androgen 11-ketotestosterone (11kT). The relative abundance of both 11kT, which binds to androgen receptors (ARα, ARβ), and cortisol, which binds to glucocorticoid receptors (GR-1, GR-2), is regulated by 11β-hydroxylase (11βH) that converts 11-deoxycortisol to cortisol and testosterone to 11β-OH-testosterone, and 11β-hydroxysteroid dehydrogenase (11βHSD) that converts cortisol to the inactive metabolite cortisone and 11β-OH-testosterone to 11kT. In midshipman fish, we tested the hypothesis that plasma steroid levels, mRNA abundance for 11βH and 11βHSD in the vocal muscle and testis (known site of 11kT synthesis), and mRNA abundances for ARs and GRs in vocal muscle, would differ between males that did or did not recently produce 'hum' advertisement calls. Quantitative real-time PCR demonstrated that non-calling male vocal muscle had significantly higher mRNA levels for all receptors except ARα, and a strong trend for higher 11βHSD; 11βH was similar to that in calling males. Calling males had higher plasma and testis 11kT, but lower plasma cortisol, levels. Testis enzyme levels did not differ between male groups, although calling males showed a positive linear correlation between plasma 11kT and testis 11βHSD mRNA levels, consistent with testis being the main source of plasma 11kT. We propose that higher vocal muscle 11βHSD levels in non-calling males reflect increased local conversion of elevated cortisol to cortisone, providing protection from cortisol-related toxicity, while increased receptor expression in non-calling males functions as a preparatory mechanism for meeting the physiological demands of future vocalization. Copyright © 2012 Elsevier Inc. All rights reserved.

  5. Optimal temporal placement of the call in beach volleyball

    Directory of Open Access Journals (Sweden)

    Stefan Künzell

    2016-05-01

    Full Text Available The call is a tactical component in beach volleyball attacks. Through the call, the setter indicates to his or her teammate an open spot in the opponent’s court. In two experimental conditions, we investigated the interval between the call and the ball-hand contact (“call shot interval”, CSI of top-level athletes. We show that the probability that a given call is followed is dependent on the duration of the CSI and the number of call options. Longer CSIs result in an increased probability that the given call will be followed, whilst increasing the call options results in a decrease in probability. On average, there is a 50% probability that the call will be followed if the call precedes the shot by 460 ms and if a single call option (“line” is expected. If the attacker has to choose between three call options (“line”, “cut”, “no-one” a 50% probability that the call will be followed is observed at an CSI of 542 ms. It did not appear that gender influenced the ability to follow a call. We recommend that in practice and in competition, players and coaches should consider the proper duration of the CSI for effective calling.

  6. Elastic calls in an integrated services network: the greater the call size variability the better the QoS

    NARCIS (Netherlands)

    Litjens, Remco; Boucherie, Richardus J.

    2003-01-01

    We study a telecommunications network integrating stream calls and elastic calls that share a given number of available traffic channels. The prioritised speech calls are handled as in a traditional Erlang loss model, while the delay-tolerant elastic calls are served with the remaining (varying)

  7. Arginine vasotocin injection increases probability of calling in cricket frogs, but causes call changes characteristic of less aggressive males.

    Science.gov (United States)

    Marler, C A; Chu, J; Wilczynski, W

    1995-12-01

    Male cricket frogs, Acris crepitans communicate to males and females using advertisement calls, which are arranged into call groups. Calls at the middle and end, but not beginning of the call group, are modified in response to male-male aggressive interactions. We found in this field study of male cricket frogs in natural breeding choruses that the peptide hormone arginine vasotocin (AVT) not only increased the probability that males called after injections, but also caused modifications in middle and end calls to produce calls characteristic of less aggressive males. Moreover, AVT-injected males showed significantly greater increases in call dominant frequency than saline-injected males, again, a characteristic of less aggressive males. Cricket frog calls are used to both repel males and attract females, thus call changes may relate to male-male and/or male-female interactions. Saline-injected males also demonstrated significant changes in several call traits, including changes that occurred in the beginning and middle calls of the call groups, but not the end calls. AVT appeared to block some call changes produced through handling. These data suggest that AVT can influence acoustic communication in frogs in several ways, including effects on call characteristics and dominant frequency, as well as potentially blocking some handling effects.

  8. Elastic calls in an integrated services network: the greater the call size variability the better the QoS

    NARCIS (Netherlands)

    Litjens, Remco; Boucherie, Richard J.

    2003-01-01

    We study a telecommunications network integrating stream calls and elastic calls that share a given number of available traffic channels. The prioritised speech calls are handled as in a traditional Erlang loss model, while the delay-tolerant elastic calls are served with the remaining (varying) ser

  9. OVarCall: Bayesian Mutation Calling Method Utilizing Overlapping Paired-End Reads.

    Science.gov (United States)

    Moriyama, Takuya; Shiraishi, Yuichi; Chiba, Kenichi; Yamaguchi, Rui; Imoto, Seiya; Miyano, Satoru

    2017-03-01

    Detection of somatic mutations from tumor and matched normal sequencing data has become a standard approach in cancer research. Although a number of mutation callers have been developed, it is still difficult to detect mutations with low allele frequency even in exome sequencing. We expect that overlapping paired-end read information is effective for this purpose, but no mutation caller has modeled overlapping information statistically in a proper form in exome sequence data. Here, we develop a Bayesian hierarchical method, OVar- Call (https://github.com/takumorizo/OVarCall), where overlapping paired-end read information improves the accuracy of low allele frequency mutation detection. Firstly, we construct two generative models: one is for reads with somatic variants generated from tumor cells and the other is for reads that does not have somatic variants but potentially includes sequence errors. Secondly, we calculate marginal likelihood for each model using a variational Bayesian algorithm to compute Bayes factor for the detection of somatic mutations. We empirically evaluated the performance of OVarCall and confirmed its better performance than other existing methods.

  10. New degradation call admission control for increasing WCDMA system capacity

    Institute of Scientific and Technical Information of China (English)

    Liu Ningqing; Lu Zhi; Gu Xuemai

    2006-01-01

    Propose a new degradation call admission control(DCAC)scheme, which can be used in wideband code division multiple access communication system. So-called degradation is that non-real time call has the characteristic of variable bit rate, so decreasing its bit rate can reduce the load of the system, consequently the system can admit new call which should be blocked when the system is close to full load, therefore new call's access probability increases. This paper brings forward design project and does system simulation, simulation proves that DCAC can effectively decrease calls' blocking probability and increase the total number of the on-line users.

  11. Effectiveness of the Call in Beach Volleyball Attacking Play

    Directory of Open Access Journals (Sweden)

    Künzell Stefan

    2014-12-01

    Full Text Available In beach volleyball the setter has the opportunity to give her or his hitter a “call”. The call intends that the setter suggests to her or his partner where to place the attack in the opponent’s court. The effectiveness of a call is still unknown. We investigated the women’s and men’s Swiss National Beach Volleyball Championships in 2011 and analyzed 2185 attacks. We found large differences between female and male players. While men called in only 38.4% of attacks, women used calls in 85.5% of attacks. If the male players followed a given call, 63% of the attacks were successful. The success rate of attacks without any call was 55.8% and 47.6% when the call was ignored. These differences were not significant (χ2(2 = 4.55, p = 0.103. In women’s beach volleyball, the rate of successful attacks was 61.5% when a call was followed, 35% for attacks without a call, and 42.6% when a call was ignored. The differences were highly significant (χ2(2 = 23.42, p < 0.0005. Taking into account the findings of the present study, we suggested that the call was effective in women’s beach volleyball, while its effect in men’s game was unclear. Considering the quality of calls we indicate that there is a significant potential to increase the effectiveness of a call.

  12. ASR performance analysis of an experimental call routing system

    CSIR Research Space (South Africa)

    Modipa, T

    2009-11-01

    Full Text Available Call routing is an important application of Automatic Speech Recognition (ASR) technology. In this paper the authors discuss the main issues affecting the performance of a call routing system and describe the ASR component of the "Auto...

  13. 76 FR 17933 - Infrastructure Protection Data Call Survey

    Science.gov (United States)

    2011-03-31

    ... SECURITY Infrastructure Protection Data Call Survey AGENCY: National Protection and Programs Directorate... Directorate, Office of Infrastructure Protection. Title: Infrastructure Protection Data Call. OMB Number: 1670.... SUMMARY: The Department of Homeland Security (DHS), National Protection and Programs Directorate...

  14. Purpose, Mission, and Context: The Call for Educating Future Leaders

    Science.gov (United States)

    Chunoo, Vivechkanand; Osteen, Laura

    2016-01-01

    This chapter calls on higher education to reclaim its role in leadership education. Specifically it examines higher education's purpose, context, and mission as clarion calls to embed leadership education throughout higher education institutions and focuses on why this is important.

  15. A General Study on the Major Features of CALL

    Institute of Scientific and Technical Information of China (English)

    喻晖

    2009-01-01

    As Computer Assisted language Learning (CALL) has become a hot issue in the second language acquisition, this paper attempts to discuss two of the important features of CALL: the student-centered and interactiveness. It is ex-plained in the paper that these two features are the most important features characterized by CALL and they can be bet-ter realized in a CALL class than in a traditional class.

  16. Environmental Influences On Diel Calling Behavior In Baleen Whales

    Science.gov (United States)

    2015-09-30

    1 DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. Environmental Influences On Diel Calling Behavior In...understanding of the social and environmental factors that influence variability in the calling behavior of baleen whales. One of the most prevalent...observations in passive acoustic recordings over scales of days to months is diel calling behavior (i.e., higher calling rates by day versus night or

  17. Advertisement calls of Bolivian species of Scinax (Amphibia, Anura, Hylidae)

    OpenAIRE

    Riva, de la, Ignacio; Márquez, Rafael; Bosch, Jaime

    1994-01-01

    The advertisement calls of eight Bolivian species of Scinax are described including information on the calling behaviour of each species. A characteristic audiospectrogram and oscillogram are presented for each species, as well as numerical information about the spectral and temporal features of the calls. Two phenograms based on the characteristics of the mating calls are constructed, the first one using a traditional multivariate technique (UPGMA) and the second one using audiospectrogram c...

  18. Xenobiotic-induced hepatocyte proliferation associated with constitutive active/androstane receptor (CAR or peroxisome proliferator-activated receptor α (PPARα is enhanced by pregnane X receptor (PXR activation in mice.

    Directory of Open Access Journals (Sweden)

    Ryota Shizu

    Full Text Available Xenobiotic-responsive nuclear receptors pregnane X receptor (PXR, constitutive active/androstane receptor (CAR and peroxisome proliferator-activated receptor α (PPARα play pivotal roles in the metabolic functions of the liver such as xenobiotics detoxification and energy metabolism. While CAR or PPARα activation induces hepatocyte proliferation and hepatocarcinogenesis in rodent models, it remains unclear whether PXR activation also shows such effects. In the present study, we have investigated the role of PXR in the xenobiotic-induced hepatocyte proliferation with or without CAR activation by 1,4-bis[2-(3,5-dichloropyridyloxy]benzene (TCPOBOP and phenobarbital, or PPARα activation by Wy-14643 in mice. Treatment with TCPOBOP or phenobarbital increased the percentage of Ki-67-positive nuclei as well as mRNA levels of cell proliferation-related genes in livers as expected. On the other hand, treatment with the PXR activator pregnenolone 16α-carbonitrile (PCN alone showed no such effects. Surprisingly, PCN co-treatment significantly augmented the hepatocyte proliferation induced by CAR activation with TCPOBOP or phenobarbital in wild-type mice but not in PXR-deficient mice. Intriguingly, PXR activation also augmented the hepatocyte proliferation induced by Wy-14643 treatment. Moreover, PCN treatment increased the RNA content of hepatocytes, suggesting the induction of G0/G1 transition, and reduced mRNA levels of Cdkn1b and Rbl2, encoding suppressors of cell cycle initiation. Our present findings indicate that xenobiotic-induced hepatocyte proliferation mediated by CAR or PPARα is enhanced by PXR co-activation despite that PXR activation alone does not cause the cell proliferation in mouse livers. Thus PXR may play a novel and unique role in the hepatocyte/liver hyperplasia upon exposure to xenobiotics.

  19. Pregnane X Receptor Activation Attenuates Inflammation-Associated Intestinal Epithelial Barrier Dysfunction by Inhibiting Cytokine-Induced Myosin Light-Chain Kinase Expression and c-Jun N-Terminal Kinase 1/2 Activation.

    Science.gov (United States)

    Garg, Aditya; Zhao, Angela; Erickson, Sarah L; Mukherjee, Subhajit; Lau, Aik Jiang; Alston, Laurie; Chang, Thomas K H; Mani, Sridhar; Hirota, Simon A

    2016-10-01

    The inflammatory bowel diseases (IBDs) are chronic inflammatory disorders with a complex etiology. IBD is thought to arise in genetically susceptible individuals in the context of aberrant interactions with the intestinal microbiota and other environmental risk factors. Recently, the pregnane X receptor (PXR) was identified as a sensor for microbial metabolites, whose activation can regulate the intestinal epithelial barrier. Mutations in NR1I2, the gene that encodes the PXR, have been linked to IBD, and in animal models, PXR deletion leads to barrier dysfunction. In the current study, we sought to assess the mechanism(s) through which the PXR regulates barrier function during inflammation. In Caco-2 intestinal epithelial cell monolayers, tumor necrosis factor-α/interferon-γ exposure disrupted the barrier and triggered zonula occludens-1 relocalization, increased expression of myosin light-chain kinase (MLCK), and activation of c-Jun N-terminal kinase 1/2 (JNK1/2). Activation of the PXR [rifaximin and [[3,5-Bis(1,1-dimethylethyl)-4-hydroxyphenyl]ethenylidene]bis-phosphonic acid tetraethyl ester (SR12813); 10 μM] protected the barrier, an effect that was associated with attenuated MLCK expression and JNK1/2 activation. In vivo, activation of the PXR [pregnenolone 16α-carbonitrile (PCN)] attenuated barrier disruption induced by toll-like receptor 4 activation in wild-type, but not Pxr-/-, mice. Furthermore, PCN treatment protected the barrier in the dextran-sulfate sodium model of experimental colitis, an effect that was associated with reduced expression of mucosal MLCK and phosphorylated JNK1/2. Together, our data suggest that the PXR regulates the intestinal epithelial barrier during inflammation by modulating cytokine-induced MLCK expression and JNK1/2 activation. Thus, targeting the PXR may prove beneficial for the treatment of inflammation-associated barrier disruption in the context of IBD.

  20. Differences in Gene Regulation by Dual Ligands of Nuclear Receptors Constitutive Androstane Receptor (CAR) and Pregnane X Receptor (PXR) in HepG2 Cells Stably Expressing CAR/PXR.

    Science.gov (United States)

    Kanno, Yuichiro; Tanuma, Nobuaki; Yazawa, Saki; Zhao, Shuai; Inaba, Miki; Nakamura, Satoshi; Nemoto, Kiyomitsu; Inouye, Yoshio

    2016-08-01

    The constitutive androstane receptor (CAR) and pregnane X receptor (PXR) regulate various genes involved in xenobiotics and drug metabolism. In many cases, CAR/PXR share ligands termed dual ligands of CAR/PXR. It is difficult to investigate the effect of CAR/PXR dual ligands in cell lines because CAR and PXR expression is scarcely detected in cultured cell lines. Here, we established a tetracycline-inducible human CAR and stably human PXR-overexpressing HepG2 cell line (HepTR/hCAR/hPXR) to examine CAR/PXR dual ligands. In the present study, we investigated the regulation of CYP2B6, CYP2C9, CYP3A4, and UDP-glucuronosyl transferase, which are target genes of CAR/PXR, by dual ligands of CAR/PXR in two transfectants. Activation of CAR and PXR in cells treated with a high dose of CITCO [6-(4-chlorophenyl)-imidazo(2,1-b)thiazole-5-carbaldehyde] or cotreated with rifampicin and tetracycline resulted in synergistic enhancement of CYP3A4, but not CYP2B6, CYP2C9, or UGT1A1, mRNA expression in HepTR/hCAR/hPXR cells. In contrast, this synergistic effect was not observed in HepTR/hCAR cells. These observations were also demonstrated in human primary hepatocytes. Taken together, our results suggest that dual ligands of CAR/PXR show distinct gene regulation patterns by cross-talk between CAR and PXR. Furthermore, the two newly established cell lines are useful tools to investigate dual ligands of CAR/PXR.

  1. 46 CFR 169.750 - Radio call sign.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Radio call sign. 169.750 Section 169.750 Shipping COAST... Control, Miscellaneous Systems, and Equipment Markings § 169.750 Radio call sign. Each vessel certificated for exposed or partially protected water service must have its radio call sign permanently...

  2. 78 FR 35956 - Utah Resource Advisory Council Subgroup Conference Call

    Science.gov (United States)

    2013-06-14

    ... Bureau of Land Management Utah Resource Advisory Council Subgroup Conference Call AGENCY: Bureau of Land Management, Interior. ACTION: Conference Call. SUMMARY: In accordance with the Federal Land Policy and... Advisory Council (RAC) Subgroup will host a conference call. DATES: The Utah RAC Subgroup will host...

  3. 75 FR 16163 - Notice of FHA Debenture Call

    Science.gov (United States)

    2010-03-31

    ... URBAN DEVELOPMENT Notice of FHA Debenture Call AGENCY: Office of the Assistant Secretary for Housing... the Secretary of the Treasury, announces the call of all FHA debentures, with a coupon rate of 5..., ``outstanding'' as of March 31, 2010. The date of the call is July 1, 2010. The debentures will be redeemed...

  4. 76 FR 17429 - Notice of FHA Debenture Call

    Science.gov (United States)

    2011-03-29

    ... URBAN DEVELOPMENT Notice of FHA Debenture Call AGENCY: Office of the Assistant Secretary for Housing--Federal Housing Commissioner, HUD. ACTION: Notice. SUMMARY: This Notice announces a debenture call of... the approval of the Secretary of the Treasury, announces the call of all FHA debentures, with a...

  5. 76 FR 43688 - Committee Meeting via Conference Call

    Science.gov (United States)

    2011-07-21

    ... HUMAN SERVICES Administration for Children and Families Committee Meeting via Conference Call AGENCY..., August 16, 2011, from 1 p.m. to 2:30 pm E.S.T. This meeting, to be held via audio conference call, is open to the public. Details for accessing the full Committee Conference Call are cited below: Toll...

  6. 29 CFR 785.17 - On-call time.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false On-call time. 785.17 Section 785.17 Labor Regulations... On-call time. An employee who is required to remain on call on the employer's premises or so close thereto that he cannot use the time effectively for his own purposes is working while “on call”....

  7. 77 FR 18258 - Notice of FHA Debenture Call

    Science.gov (United States)

    2012-03-27

    ... URBAN DEVELOPMENT Notice of FHA Debenture Call AGENCY: Office of the Assistant Secretary for Housing--Federal Housing Commissioner, HUD. ACTION: Notice. SUMMARY: This Notice announces a debenture call of... impairments may access this number through TTY by calling the toll-free Federal Relay Service at...

  8. Linguistics, Procedure and Technique in CALL Teacher Education

    Science.gov (United States)

    Torsani, Simone

    2015-01-01

    Research in CALL education has identified a series of goals and constraints for technology in language education that need to be taken into account when designing a CALL syllabus. This article presents a theoretical framework for syllabus design based on the identification of three areas of expertise within CALL: linguistic knowledge, procedural…

  9. Evolution of advertisement calls in African clawed frogs

    Science.gov (United States)

    Tobias, Martha L.; Evans, Ben J.; Kelley, Darcy B.

    2014-01-01

    Summary For most frogs, advertisement calls are essential for reproductive success, conveying information on species identity, male quality, sexual state and location. While the evolutionary divergence of call characters has been examined in a number of species, the relative impacts of genetic drift or natural and sexual selection remain unclear. Insights into the evolutionary trajectory of vocal signals can be gained by examining how advertisement calls vary in a phylogenetic context. Evolution by genetic drift would be supported if more closely related species express more similar songs. Conversely, a poor correlation between evolutionary history and song expression would suggest evolution shaped by natural or sexual selection. Here, we measure seven song characters in 20 described and two undescribed species of African clawed frogs (genera Xenopus and Silurana) and four populations of X. laevis. We identify three call types — click, burst and trill — that can be distinguished by click number, call rate and intensity modulation. A fourth type is biphasic, consisting of two of the above. Call types vary in complexity from the simplest, a click, to the most complex, a biphasic call. Maximum parsimony analysis of variation in call type suggests that the ancestral type was of intermediate complexity. Each call type evolved independently more than once and call type is typically not shared by closely related species. These results indicate that call type is homoplasious and has low phylogenetic signal. We conclude that the evolution of call type is not due to genetic drift, but is under selective pressure. PMID:24723737

  10. Calling and Career Preparation: Investigating Developmental Patterns and Temporal Precedence

    Science.gov (United States)

    Hirschi, Andreas; Herrmann, Anne

    2013-01-01

    The presence of a calling and career development are assumed to be closely related. However, the nature of and reason for this relationship have not been thoroughly investigated. We hypothesized the existence of reciprocal effects between calling and three dimensions of career preparation and assessed the change of the presence of a calling,…

  11. Evolution of advertisement calls in African clawed frogs.

    Science.gov (United States)

    Tobias, Martha L; Evans, Ben J; Kelley, Darcy B

    2011-01-01

    For most frogs, advertisement calls are essential for reproductive success, conveying information on species identity, male quality, sexual state and location. While the evolutionary divergence of call characters has been examined in a number of species, the relative impacts of genetic drift or natural and sexual selection remain unclear. Insights into the evolutionary trajectory of vocal signals can be gained by examining how advertisement calls vary in a phylogenetic context. Evolution by genetic drift would be supported if more closely related species express more similar songs. Conversely, a poor correlation between evolutionary history and song expression would suggest evolution shaped by natural or sexual selection. Here, we measure seven song characters in 20 described and two undescribed species of African clawed frogs (genera Xenopus and Silurana) and four populations of X. laevis. We identify three call types - click, burst and trill - that can be distinguished by click number, call rate and intensity modulation. A fourth type is biphasic, consisting of two of the above. Call types vary in complexity from the simplest, a click, to the most complex, a biphasic call. Maximum parsimony analysis of variation in call type suggests that the ancestral type was of intermediate complexity. Each call type evolved independently more than once and call type is typically not shared by closely related species. These results indicate that call type is homoplasious and has low phylogenetic signal. We conclude that the evolution of call type is not due to genetic drift, but is under selective pressure.

  12. 47 CFR 90.241 - Radio call box operations.

    Science.gov (United States)

    2010-10-01

    ... remains on for a period in excess of three minutes. The automatic cutoff system must be designed so the... Safety Pool for highway call box systems subject to the following requirements: (1) Call box transmitters... effective radiated power (ERP). (3) The height of a call box antenna may not exceed 6.1 meters (20...

  13. 47 CFR 25.284 - Emergency Call Center Service.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Emergency Call Center Service. 25.284 Section... SATELLITE COMMUNICATIONS Technical Operations § 25.284 Emergency Call Center Service. (a) Providers of mobile satellite service to end-user customers (part 25, subparts A-D) must provide Emergency Call...

  14. 26 CFR 1.1092(c)-1 - Qualified covered calls.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) INCOME TAXES Wash Sales of Stock Or Securities § 1.1092(c)-1 Qualified covered calls. (a) In... equity call option with standardized terms on Corporation X stock will not be deep in the money if the... equity call option with standardized terms on Corporation Y stock will not be deep in the money if the...

  15. Pricing of convertible bonds with hard call features

    NARCIS (Netherlands)

    Wever, Jolle O.; Smid, Peter P.M.; Koning, Ruud H.

    2002-01-01

    This paper discusses the development of a valuation model for convertible bonds with hard call features. We define a hard call feature as the possibility for the issuer to redeem a convertible bond before maturity by paying the call price to the bondholder. We use the binomial approach to model conv

  16. The So-Called 'Face on Mars'

    Science.gov (United States)

    2002-01-01

    (Released 13 April 2002) The Science The so called 'Face on Mars' can be seen slightly above center and to the right in this THEMIS visible image. This 3-km long knob, located near 10o N, 40o W (320o E), was first imaged by the Viking spacecraft in the 1970's and was seen by some to resemble a face carved into the rocks of Mars. Since that time the Mars Orbiter Camera on the Mars Global Surveyor spacecraft has provided detailed views of this hill that clearly show that it is a normal geologic feature with slopes and ridges carved by eons of wind and downslope motion due to gravity. A similar-size hill in Phoenix, Arizona resembles a camel lying on the ground, and Phoenicians whimsically refer to it as Camelback Mountain. Like the hills and knobs of Mars, however, Camelback Mountain was carved into its unusual shape by thousands of years of erosion. The THEMIS image provides a broad perspective of the landscape in this region, showing numerous knobs and hills that have been eroded into a remarkable array of different shapes. Many of these knobs, including the 'Face', have several flat ledges partway up the hill slopes. These ledges are made of more resistant layers of rock and are the last remnants of layers that once were continuous across this entire region. Erosion has completely removed these layers in most places, leaving behind only the small isolated hills and knobs seen today. Many of the hills and ridges in this area also show unusual deposits of material that occur preferentially on the cold, north-facing slopes. It has been suggested that these deposits were 'pasted' on the slopes, with the distinct, rounded boundary on their upslope edges being the highest remaining point of this pasted-on layer. In several locations, such as in the large knob directly south of the 'Face', these deposits occur at several different heights on the hill. This observation suggests the layer once draped the entire knob and has since been removed from all but the north

  17. Una calle nunca es una calle: el mundo de los frigoríficos

    OpenAIRE

    Moschen, Susana; Rodríguez Iozza, Gisela B.; Scaglia, Roxana B.; Dobarro, Claudia; Omelusik, M. Alejandra

    2008-01-01

    Repensando nuestra práctica docente, desde lo trabajado en el transcurso de la Especial Preparación correspondiente al área de Ciencias Sociales y en el Seminario: Patrimonio y Educación Preuniversitaria, es que nos propusimos la incorporación al Proyecto de Ciencias Sociales, como estudio de caso: los frigoríficos Swift y Armour. Se trata de un sitio histórico, una de las nominaciones a la lista patrimonial existente a nivel nacional, a escala de la microrregión del Gran La Plata; la calle N...

  18. Elastic calls in an integrated services network: the greater the call size variability the better the QoS

    NARCIS (Netherlands)

    Litjens, R.; Boucherie, Richardus J.

    2002-01-01

    We study a telecommunications network integrating prioritized stream calls and delay tolerant elastic calls that are served with the remaining (varying) service capacity according to a processor sharing discipline. The remarkable observation is presented and analytically supported that the expected

  19. The Phonetics and Phonology of the Polish Calling Melodies.

    Science.gov (United States)

    Arvaniti, Amalia; Żygis, Marzena; Jaskuła, Marek

    2016-01-01

    Two calling melodies of Polish were investigated, the routine call, used to call someone for an everyday reason, and the urgent call, which conveys disapproval of the addressee's actions. A Discourse Completion Task was used to elicit the two melodies from Polish speakers using twelve names from one to four syllables long; there were three names per syllable count, and speakers produced three tokens of each name with each melody. The results, based on eleven speakers, show that the routine calling melody consists of a low F0 stretch followed by a rise-fall-rise; the urgent calling melody, on the other hand, is a simple rise-fall. Systematic differences were found in the scaling and alignment of tonal targets: the routine call showed late alignment of the accentual pitch peak, and in most instances lower scaling of targets. The accented vowel was also affected, being overall louder in the urgent call. Based on the data and comparisons with other Polish melodies, we analyze the routine call as LH* !H-H% and the urgent call as H* L-L%. We discuss the results and our analysis in light of recent findings on calling melodies in other languages, and explore their repercussions for intonational phonology and the modeling of intonation. © 2017 S. Karger AG, Basel.

  20. Individual distinctiveness in call types of wild western female gorillas.

    Directory of Open Access Journals (Sweden)

    Roberta Salmi

    Full Text Available Individually distinct vocalizations play an important role in animal communication, allowing call recipients to respond differentially based on caller identity. However, which of the many calls in a species' repertoire should have more acoustic variability and be more recognizable is less apparent. One proposed hypothesis is that calls used over long distances should be more distinct because visual cues are not available to identify the caller. An alternative hypothesis proposes that close calls should be more recognizable because of their importance in social interactions. To examine which hypothesis garners more support, the acoustic variation and individual distinctiveness of eight call types of six wild western gorilla (Gorilla gorilla females were investigated. Acoustic recordings of gorilla calls were collected at the Mondika Research Center (Republic of Congo. Acoustic variability was high in all gorilla calls. Similar high inter-individual variation and potential for identity coding (PIC was found for all call types. Discriminant function analyses confirmed that all call types were individually distinct (although for call types with lowest sample size - hum, grumble and scream - this result cannot be generalized, suggesting that neither the distance at which communication occurs nor the call social function alone can explain the evolution of identity signaling in western gorilla communication.

  1. The Barbados Emergency Ambulance Service: High Frequency of Nontransported Calls

    Directory of Open Access Journals (Sweden)

    Sherwin E. Phillips

    2012-01-01

    Full Text Available Objectives. There are no published studies on the Barbados Emergency Ambulance Service and no assessment of the calls that end in nontransported individuals. We describe reasons for the nontransport of potential clients. Methods. We used the Emergency Medical Dispatch (Medical Priority Dispatch System instrument, augmented with five local call types, to collect information on types of calls. The calls were categorised under 7 headings. Correlations between call types and response time were calculated. Results. Most calls were from the category medical (54%. Nineteen (19% percent of calls were in the non-transported category. Calls from call type Cancelled accounted for most of these and this was related to response time, while Refused service was inversely related (. Conclusions. The Barbados Ambulance Service is mostly used by people with a known illness and for trauma cases. One-fifth of calls fall into a category where the ambulance is not used often due to cancellation which is related to response time. Other factors such as the use of alternative transport are also important. Further study to identify factors that contribute to the non-transported category of calls is necessary if improvements in service quality are to be made.

  2. Acoustic structures in the alarm calls of Gunnison's prairie dogs.

    Science.gov (United States)

    Slobodchikoff, C N; Placer, J

    2006-05-01

    Acoustic structures of sound in Gunnison's prairie dog alarm calls are described, showing how these acoustic structures may encode information about three different predator species (red-tailed hawk-Buteo jamaicensis; domestic dog-Canis familaris; and coyote-Canis latrans). By dividing each alarm call into 25 equal-sized partitions and using resonant frequencies within each partition, commonly occurring acoustic structures were identified as components of alarm calls for the three predators. Although most of the acoustic structures appeared in alarm calls elicited by all three predator species, the frequency of occurrence of these acoustic structures varied among the alarm calls for the different predators, suggesting that these structures encode identifying information for each of the predators. A classification analysis of alarm calls elicited by each of the three predators showed that acoustic structures could correctly classify 67% of the calls elicited by domestic dogs, 73% of the calls elicited by coyotes, and 99% of the calls elicited by red-tailed hawks. The different distributions of acoustic structures associated with alarm calls for the three predator species suggest a duality of function, one of the design elements of language listed by Hockett [in Animal Sounds and Communication, edited by W. E. Lanyon and W. N. Tavolga (American Institute of Biological Sciences, Washington, DC, 1960), pp. 392-430].

  3. Acoustic Characteristics of Advertisement Calls in Babina adenopleura

    Institute of Scientific and Technical Information of China (English)

    Xiaobin FANG; Xia QIU; Yilin ZHOU; Luyi YANG; Yi ZHAO; Weihong ZHENG; Jinsong LIU

    2015-01-01

    Acoustic communication is the most important form of communication in anuran amphibians. To understand the acoustic characteristics of male Babina adenopleura, we recorded advertisement calls and analyzed their acoustic parameters during the breeding season. Male B. adenopleura produced calls with a variable number of notes (1–5), and each note contained harmonics. Although 6%of call notes did not exhibit frequency modulation (FM), two call note FM patterns were observed:(1) upward FM;(2) upward–downward FM. With the exception of 1-and 5-note calls, the duration of successive notes decreased monotonically. With the exception of 1 note calls, the fundamental frequency of the first note was lowest, then increased; the greatest change in the fundamental frequency was always between notes 1 and 2. The dominant frequency varied between calls. For example for the ifrst call note the dominant frequency occurred in some cases in the ifrst harmonic (located in the 605.320 ± 64.533 Hz frequency band), the second harmonic (918 ± 9 Hz band), the fourth harmonic (1712 ± 333 Hz band), the sixth harmonic (the 2165 ± 152 Hz band), the seventh harmonic (the 2269 ± 140 Hz band), the eighth harmonic (the 2466 ± 15 Hz band) or the ninth harmonic (the 2636 ± 21 Hz band). Although male B. adenopleura advertisement calls have a distinctive structure, they have similar characteristics to the calls of the music frog, B. daunchina.

  4. In- and Out-of-the-Money Convertible Bond Calls

    DEFF Research Database (Denmark)

    Bechman, Ken; Lunde, Asger; Zebedee, Allan

    2014-01-01

    Convertible bond calls typically cause significant reactions in equity prices. The empirical research largely finds negative and positive announcement effects for the in-the-money and the out-of-the-money calls respectively. However, this research has difficulty distinguishing between the two main...... theoretical explanations: the signaling effect and the price pressure effect. In this paper, we differentiate between these two effects by using a unique data set of the in- and the out-of-the-money calls in the United States during the period of 1993 to 2007. We find that the announcement effect for the in......-the-money call is predominantly explained by the subsequent order imbalances; and the stock market's reaction is spread over an entire trading day, which is consistent with the price pressure effect. In contrast, the announcement effect for the out-of-the-money call is driven by the size of the called...

  5. Modeling synchronized calling behavior of Japanese tree frogs

    Science.gov (United States)

    Aihara, Ikkyu

    2009-07-01

    We experimentally observed synchronized calling behavior of male Japanese tree frogs Hyla japonica; namely, while isolated single frogs called nearly periodically, a pair of interacting frogs called synchronously almost in antiphase or inphase. In this study, we propose two types of phase-oscillator models on different degrees of approximations, which can quantitatively explain the phase and frequency properties in the experiment. Moreover, it should be noted that, although the second model is obtained by fitting to the experimental data of the two synchronized states, the model can also explain the transitory dynamics in the interactive calling behavior, namely, the shift from a transient inphase state to a stable antiphase state. We also discuss the biological relevance of the estimated parameter values to calling behavior of Japanese tree frogs and the possible biological meanings of the synchronized calling behavior.

  6. Voice and daytime calling of Scops Owls (Otus scops

    Directory of Open Access Journals (Sweden)

    Mikkola Anita

    2015-12-01

    Full Text Available The Scops Owl (Otus scops is one of the least studied owls in Europe and its loud and monotonous calls are not well documented. This paper summarises published alarm-like calls and presents data on daytime calling recorded in Lesencetomaj, Hungary between 9th and 17th September 2012. Calls were similar to the sound of an alarm on a reversing truck. The bird was most active around midday (11–13 hrs and in the afternoons between 15–17 hrs. No calling occurred before 09.00 or after 19.15 o’clock. Weather affected the intensity of calls and particularly high autumn temperatures are suggested as a possible reason for this unusual activity.

  7. Modular Design of Call Control Layer in Telephony Software

    Directory of Open Access Journals (Sweden)

    Ilija Basicevic

    2011-01-01

    Full Text Available An important property of a telephony system is the call control model on which it is based. It is noted that many call control models in the past, especially those in PSTN/ISDN networks follow centralized model. For such a model, typical is significant coupling of modules belonging to different services with the basic call control module which is aware of all active telephony features in the system. Although sometimes based on distributed model, VoIP call control models still manifest some of the listed problems of their predecessors. In this paper we present a fully distributed model which exhibits minimal coupling of modules belonging to different services and a simple basic call control module. The model is based on taxonomies of call control services which are presented in the paper. Also, the implementation of several typical services is described.

  8. A synthetic operational account of call-by-need evaluation

    DEFF Research Database (Denmark)

    Zerny, Ian; Danvy, Olivier

    2013-01-01

    We present the first operational account of call by need that connects syntactic theory and implementation practice. Syntactic theory: the storeless operational semantics using syntax rewriting to account for demand-driven computation and for caching intermediate results. Implementational practice...... is that the theory of call by need agrees with the theory of call by name, and that the practice of call by need optimizes the practice of call by name. Our operational account takes the form of three new calculi for lazy evaluation of lambda-terms and our synthesis takes the form of three lock-step equivalences......-terms. Our results reveal a genuine and principled unity of computational theory and computational practice, one that readily applies to variations on the general theme of call by need....

  9. 40 CFR 155.48 - Data Call-In.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Data Call-In. 155.48 Section 155.48... STANDARDS AND REGISTRATION REVIEW Registration Review Procedures § 155.48 Data Call-In. The Agency may issue a Data Call-In notice under FIFRA section 3(c)(2)(B) at any time if the Agency believes that...

  10. The Influence of Judgment Calls on Meta-Analytic Findings.

    Science.gov (United States)

    Tarrahi, Farid; Eisend, Martin

    2016-01-01

    Previous research has suggested that judgment calls (i.e., methodological choices made in the process of conducting a meta-analysis) have a strong influence on meta-analytic findings and question their robustness. However, prior research applies case study comparison or reanalysis of a few meta-analyses with a focus on a few selected judgment calls. These studies neglect the fact that different judgment calls are related to each other and simultaneously influence the outcomes of a meta-analysis, and that meta-analytic findings can vary due to non-judgment call differences between meta-analyses (e.g., variations of effects over time). The current study analyzes the influence of 13 judgment calls in 176 meta-analyses in marketing research by applying a multivariate, multilevel meta-meta-analysis. The analysis considers simultaneous influences from different judgment calls on meta-analytic effect sizes and controls for alternative explanations based on non-judgment call differences between meta-analyses. The findings suggest that judgment calls have only a minor influence on meta-analytic findings, whereas non-judgment call differences between meta-analyses are more likely to explain differences in meta-analytic findings. The findings support the robustness of meta-analytic results and conclusions.

  11. Extracts of Immature Orange (Aurantii fructus immaturus) and Citrus Unshiu Peel (Citri unshiu pericarpium) Induce P-Glycoprotein and Cytochrome P450 3A4 Expression via Upregulation of Pregnane X Receptor.

    Science.gov (United States)

    Okada, Naoto; Murakami, Aki; Urushizaki, Shiori; Matsuda, Misa; Kawazoe, Kazuyoshi; Ishizawa, Keisuke

    2017-01-01

    P-glycoprotein (P-gp) and cytochrome P450 3A4 (CYP3A4) are expressed in the intestine and are associated with drug absorption and metabolism. Pregnane X receptor (PXR) is the key molecule that regulates the expression of P-gp and CYP3A4. Given that PXR activity is regulated by a variety of compounds, it is possible that unknown PXR activators exist among known medicines. Kampo is a Japanese traditional medicine composed of various natural compounds. In particular, immature orange [Aurantii fructus immaturus (IO)] and citrus unshiu peel [Citri unshiu pericarpium (CP)] are common ingredients of kampo. A previous study reported that kampo containing IO or CP decreased the blood concentration of concomitant drugs via upregulation of CYP3A4 although the mechanism was unclear. Some flavonoids are indicated to alter P-gp and CYP3A4 activity via changes in PXR activity. Because IO and CP include various flavonoids, we speculated that the activity of P-gp and CYP3A4 in the intestine may be altered via changes in PXR activity when IO or CP is administered. We tested this hypothesis by using LS180 intestinal epithelial cells. The ethanol extract of IO contained narirutin and naringin, and that of CP contained narirutin and hesperidin. Ethanol extracts of IO and CP induced P-gp, CYP3A4, and PXR expression. The increase of P-gp and CYP3A4 expression by the IO and CP ethanol extracts was inhibited by ketoconazole, an inhibitor of PXR activation. The ethanol extract of IO and CP decreased the intracellular concentration of digoxin, a P-gp substrate, and this decrease was inhibited by cyclosporine A, a P-gp inhibitor. In contrast, CP, but not IO, stimulated the metabolism of testosterone, a CYP3A4 substrate, and this was inhibited by a CYP3A4 inhibitor. These findings indicate that the ethanol extract of IO and CP increased P-gp and CYP3A4 expression via induction of PXR protein. Moreover, this induction decreased the intracellular substrate concentration.

  12. Extracts of Immature Orange (Aurantii fructus immaturus) and Citrus Unshiu Peel (Citri unshiu pericarpium) Induce P-Glycoprotein and Cytochrome P450 3A4 Expression via Upregulation of Pregnane X Receptor

    Science.gov (United States)

    Okada, Naoto; Murakami, Aki; Urushizaki, Shiori; Matsuda, Misa; Kawazoe, Kazuyoshi; Ishizawa, Keisuke

    2017-01-01

    P-glycoprotein (P-gp) and cytochrome P450 3A4 (CYP3A4) are expressed in the intestine and are associated with drug absorption and metabolism. Pregnane X receptor (PXR) is the key molecule that regulates the expression of P-gp and CYP3A4. Given that PXR activity is regulated by a variety of compounds, it is possible that unknown PXR activators exist among known medicines. Kampo is a Japanese traditional medicine composed of various natural compounds. In particular, immature orange [Aurantii fructus immaturus (IO)] and citrus unshiu peel [Citri unshiu pericarpium (CP)] are common ingredients of kampo. A previous study reported that kampo containing IO or CP decreased the blood concentration of concomitant drugs via upregulation of CYP3A4 although the mechanism was unclear. Some flavonoids are indicated to alter P-gp and CYP3A4 activity via changes in PXR activity. Because IO and CP include various flavonoids, we speculated that the activity of P-gp and CYP3A4 in the intestine may be altered via changes in PXR activity when IO or CP is administered. We tested this hypothesis by using LS180 intestinal epithelial cells. The ethanol extract of IO contained narirutin and naringin, and that of CP contained narirutin and hesperidin. Ethanol extracts of IO and CP induced P-gp, CYP3A4, and PXR expression. The increase of P-gp and CYP3A4 expression by the IO and CP ethanol extracts was inhibited by ketoconazole, an inhibitor of PXR activation. The ethanol extract of IO and CP decreased the intracellular concentration of digoxin, a P-gp substrate, and this decrease was inhibited by cyclosporine A, a P-gp inhibitor. In contrast, CP, but not IO, stimulated the metabolism of testosterone, a CYP3A4 substrate, and this was inhibited by a CYP3A4 inhibitor. These findings indicate that the ethanol extract of IO and CP increased P-gp and CYP3A4 expression via induction of PXR protein. Moreover, this induction decreased the intracellular substrate concentration. PMID:28270768

  13. Chronic intermittent ethanol exposure alters stress effects on (3α,5α-3-hydroxy-pregnan-20-one (3α,5α-THP immunolabeling of amygdala neurons in C57BL/6J mice

    Directory of Open Access Journals (Sweden)

    Antoniette M Maldonado-Devincci

    2016-03-01

    Full Text Available The GABAergic neuroactive steroid (3α,5α-3-hydroxy-pregnan-20-one (3α,5α-THP, allopregnanolone is decreased in various brain regions of C57BL/6J mice following exposure to an acute stressor or chronic intermittent ethanol (CIE exposure and withdrawal. It is well established that there are complex interactions between stress and ethanol drinking, with mixed literature regarding the effects of stress on ethanol intake. However, there is little research examining how chronic ethanol exposure alters stress responses. The present work examined the impact of CIE exposure and withdrawal on changes in brain levels of 3α,5α-THP, hormonal, and behavioral responses to forced swim stress (FSS. Adult male C57BL/6J mice were exposed to four cycles of CIE to induce ethanol dependence. Following 8 or 72 hr withdrawal, mice were subjected to FSS for 10 min, and 50 min later brains were collected for immunohistochemical analysis of cellular 3α,5α-THP. Behavioral and circulating corticosterone responses to the FSS were quantified. Following 8 hr withdrawal, ethanol exposure potentiated the corticosterone response to FSS. Following 72 hr withdrawal, this difference was no longer observed. Following 8 hr withdrawal, stress-exposed mice showed no differences in immobility, swimming or struggling behavior. However, following 72 hr withdrawal, ethanol-exposed mice showed less immobility and greater swimming behavior compared to air-exposed mice. Interestingly, cellular 3α,5α-THP levels were increased in the lateral amygdala 8 hr and 72 hr post-withdrawal in stressed ethanol-exposed mice compared to ethanol-exposed/non-stressed mice. In the paraventricular nucleus of the hypothalamus, stress exposure decreased 3α,5α-THP levels compared to controls following 72 hr withdrawal, but no differences were observed 8 hr post-withdrawal. There were no differences in cellular 3α,5α-THP levels in the nucleus accumbens shell at either withdrawal time point. These data

  14. Female chimpanzees use copulation calls flexibly to prevent social competition.

    Directory of Open Access Journals (Sweden)

    Simon W Townsend

    Full Text Available The adaptive function of copulation calls in female primates has been debated for years. One influential idea is that copulation calls are a sexually selected trait, which enables females to advertise their receptive state to males. Male-male competition ensues and females benefit by getting better mating partners and higher quality offspring. We analysed the copulation calling behaviour of wild female chimpanzees (Pan troglodytes schweinfurthii at Budongo Forest, Uganda, but found no support for the male-male competition hypothesis. Hormone analysis showed that the calling behaviour of copulating females was unrelated to their fertile period and likelihood of conception. Instead, females called significantly more while with high-ranking males, but suppressed their calls if high-ranking females were nearby. Copulation calling may therefore be one potential strategy employed by female chimpanzees to advertise receptivity to high-ranked males, confuse paternity and secure future support from these socially important individuals. Competition between females can be dangerously high in wild chimpanzees, and our results indicate that females use their copulation calls strategically to minimise the risks associated with such competition.

  15. How can I help you? User instructions in telephone calls

    NARCIS (Netherlands)

    Steehouder, Michael; Hartman, Daniel

    2003-01-01

    We a small corpus of instructions given in phone calls to customers who need support for programming their universal remote control, to make it suitable for their particular TV set VCR, Audio, etc. Typically, in these calls the operator or 'agent' coaches the client while the client is performing ac

  16. 77 FR 12646 - Government Securities: Call for Large Position Reports

    Science.gov (United States)

    2012-03-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Government Securities: Call for Large Position Reports AGENCY: Office of the Assistant Secretary for... ``Treasury'') called for the submission of Large Position Reports by those entities whose...

  17. 78 FR 17469 - Government Securities: Call for Large Position Reports

    Science.gov (United States)

    2013-03-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Government Securities: Call for Large Position Reports AGENCY: Office of the Assistant Secretary for... ``Treasury'') called for the submission of Large Position Reports by those entities whose...

  18. 75 FR 77955 - Government Securities: Call for Large Position Reports

    Science.gov (United States)

    2010-12-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Government Securities: Call for Large Position Reports AGENCY: Office of the Assistant Secretary for... ``Treasury'') called for the submission of Large Position Reports by those entities whose...

  19. Environmental constraints and call evolution in torrent-dwelling frogs.

    Science.gov (United States)

    Goutte, Sandra; Dubois, Alain; Howard, Samuel D; Marquez, Rafael; Rowley, Jodi J L; Dehling, J Maximilian; Grandcolas, Philippe; Rongchuan, Xiong; Legendre, Frédéric

    2016-04-01

    Although acoustic signals are important for communication in many taxa, signal propagation is affected by environmental properties. Strong environmental constraints should drive call evolution, favoring signals with greater transmission distance and content integrity in a given calling habitat. Yet, few empirical studies have verified this prediction, possibly due to a shortcoming in habitat characterization, which is often too broad. Here we assess the potential impact of environmental constraints on the evolution of advertisement call in four groups of torrent-dwelling frogs in the family Ranidae. We reconstruct the evolution of calling site preferences, both broadly categorized and at a finer scale, onto a phylogenetic tree for 148 species with five markers (∼3600 bp). We test models of evolution for six call traits for 79 species with regard to the reconstructed history of calling site preferences and estimate their ancestral states. We find that in spite of existing morphological constraints, vocalizations of torrent-dwelling species are most probably constrained by the acoustic specificities of torrent habitats and particularly their high level of ambient noise. We also show that a fine-scale characterization of calling sites allows a better perception of the impact of environmental constraints on call evolution.

  20. Dialogue-Based CALL: An Overview of Existing Research

    Science.gov (United States)

    Bibauw, Serge; François, Thomas; Desmet, Piet

    2015-01-01

    Dialogue-based Computer-Assisted Language Learning (CALL) covers applications and systems allowing a learner to practice the target language in a meaning-focused conversational activity with an automated agent. We first present a common definition for dialogue-based CALL, based on three features: dialogue as the activity unit, computer as the…

  1. Evaluation of Static JavaScript Call Graph Algorithms

    NARCIS (Netherlands)

    Dijkstra, J.-J.

    2014-01-01

    This thesis consists of a replication study in which two algorithms to compute JavaScript call graphs have been implemented and evaluated. Existing IDE support for JavaScript is hampered due to the dynamic nature of the language. Previous studies partially solve call graph computation for JavaScript

  2. 76 FR 14684 - Call for Nominations for Resource Advisory Councils

    Science.gov (United States)

    2011-03-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Land Management Call for Nominations for Resource Advisory Councils AGENCY: Bureau of Land Management, Interior. ACTION: Call for nominations. SUMMARY: The purpose of this notice is to request...

  3. 39 CFR 6.4 - Attendance by conference telephone call.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Attendance by conference telephone call. 6.4 Section 6.4 Postal Service UNITED STATES POSTAL SERVICE THE BOARD OF GOVERNORS OF THE U.S. POSTAL SERVICE MEETINGS (ARTICLE VI) § 6.4 Attendance by conference telephone call. For regularly scheduled meetings...

  4. 75 FR 14622 - Call for Nominations for Resource Advisory Councils

    Science.gov (United States)

    2010-03-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Land Management Call for Nominations for Resource Advisory Councils AGENCY: Bureau of Land Management, Interior. ACTION: Notice of Resource Advisory Council Call for Nominations. SUMMARY: The...

  5. Establishing Self-access with CALL in Chinese Tertiary Education

    Institute of Scientific and Technical Information of China (English)

    WangYuwen; LiHeng

    2004-01-01

    This paper aims tO bridge up the gap between theoretical perspectives of self-access language learning (SALL) and practice of computer assisted language learning (CALL) in Chinese tertiary education. While much of the paper presented ideas dealing with issues related to SALL and CALL, they are supported by references to relevant literature and research.

  6. Call Centres in Denmark 2004 - Strategy, HR Practices & Performance

    DEFF Research Database (Denmark)

    Sørensen, Ole H.; El-Salanti, Nadia

    This survey is the first scientifically based benchmarking study of the Danish call centre industry. The main results from the study are based on answers from 128 call centres, which correspond to a response rate of 65%. The Danish study is part of a global project coordinated by researchers at C...

  7. Steven MacCall: Winner of LJ's 2010 Teaching Award

    Science.gov (United States)

    Berry, John N., III

    2010-01-01

    This article profiles Steven L. MacCall, winner of "Library Journal's" 2010 Teaching Award. An associate professor at the School of Library and Information Studies (SLIS) at the University of Alabama, Tuscaloosa, MacCall was nominated by Kathie Popadin, known as "Kpop" to the members of her cohort in the online MLIS program at SLIS. Sixteen of…

  8. Called to Teach: A Study of Highly Motivated Preservice Teachers.

    Science.gov (United States)

    Serow, Robert C.

    1994-01-01

    Examines the belief that teaching is not merely a job but a calling, analyzing survey and interview data from 527 preservice teachers. Compared to other preservice teachers, those who felt a calling displayed significantly more enthusiasm, commitment to teaching, and willingness to accept sacrifices and extra duties. (SM)

  9. Advertisement calls of Bolivian species of Scinax (Amphibia, Anura, Hylidae)

    NARCIS (Netherlands)

    Riva, de la Ignacio; Márquez, Rafael; Bosch, Jaime

    1994-01-01

    The advertisement calls of eight Bolivian species of Scinax are described including information on the calling behaviour of each species. A characteristic audiospectrogram and oscillogram are presented for each species, as well as numerical information about the spectral and temporal features of the

  10. Called to Do Meaningful Work: A Blessing or a Curse?

    Science.gov (United States)

    van Vuuren, Mark

    2017-01-01

    Two groups of people are particularly inclined to mention a calling when talking about their work motivation: those who are spiritual (because the concept of calling originated in the religious realm) and those in serving occupations (such as hospitals, schools, and nongovernmental organizations). Because Christian professors are in both groups,…

  11. Impact of Using CALL on Iranian EFL Learners' Vocabulary Knowledge

    Science.gov (United States)

    Yunus, Melor Md; Salehi, Hadi; Amini, Mahdi

    2016-01-01

    Computer Assisted Language Learning (CALL) integration in EFL contexts has intensified noticeably in recent years. This integration might be in different ways and for different purposes such as vocabulary acquisition, grammar learning, phonology, writing skills, etc. More explicitly, this study is an attempt to explore the effect of using CALL on…

  12. Advertisement calls of Bolivian species of Scinax (Amphibia, Anura, Hylidae)

    NARCIS (Netherlands)

    Riva, de la Ignacio; Márquez, Rafael; Bosch, Jaime

    1994-01-01

    The advertisement calls of eight Bolivian species of Scinax are described including information on the calling behaviour of each species. A characteristic audiospectrogram and oscillogram are presented for each species, as well as numerical information about the spectral and temporal features of the

  13. Calls to Teen Line: Representative Concerns of Adolescents.

    Science.gov (United States)

    Boehm, Kathryn E.; Schondel, Connie K.; Ivoska, William J.; Marlowe, Alison L.; Manke-Mitchell, Laurie

    1998-01-01

    Study examines whether the concerns of teenagers calling a peer listening service are representative of the concerns of teenagers in the area served. Results indicate that students' biggest concerns involve family problems, peer relationships, self-esteem, and school problems. Concludes that calls to the teen line are representative. (Author/GCP)

  14. 29 CFR 778.221 - “Call-back” pay.

    Science.gov (United States)

    2010-07-01

    ... minimum “call-back” or “call-out” payments made pursuant to employment agreements. Typically, such minimum... illustrated as follows: An employment agreement provides a minimum of 3 hours' pay at time and one-half for... Friday, inclusive, in a workweek beginning on Monday, and are paid overtime compensation at time and...

  15. Immunisation hotline calls as five-in-one vaccine introduced.

    Science.gov (United States)

    Fisher-Jeffes, Lisa; Finlay, Fiona

    2006-04-01

    Announcement of the introduction of the five-in-one vaccine (DTaP/IPV/Hib) into the primary immunisation schedule was made on 9 August 2004. In this study all calls to the immunisation hotline were recorded between 9 August 2004 and 19 November 2004, noting who called and the nature of their enquiry. A total of 208 calls were received during the study period, and of these 23 (11.1%) related to the new vaccine. Calls were from parents (10/23, 43%), health visitors (9/23, 39%) and practice nurses (3/23, 13%). A variety of themes were covered in calls including local availability of the five-in-one vaccine, vaccine safety, mercury content and efficacy. Calls not connected with the new vaccine concerned mostly adolescent MMR (17.3%) as there was a local mumps epidemic. Others related to clarification of a child's immunisation status (13.5%), primary MMR immunisation (13.5%), vaccination scheduling or administration difficulties (12%), other schedule (12.5%) and non-schedule vaccines (2.4%), vaccine reactions (2.4%), travel vaccines (6%), BCG (6%), and a few miscellaneous queries (3%). Overall questions about the new five-in-one vaccine accounted for an extra 23 calls to the immunisation hotline during the study period (11.1% of calls).

  16. Sustainability in CALL Learning Environments: A Systemic Functional Grammar Approach

    Science.gov (United States)

    McDonald, Peter

    2014-01-01

    This research aims to define a sustainable resource in Computer-Assisted Language Learning (CALL). In order for a CALL resource to be sustainable it must work within existing educational curricula. This feature is a necessary prerequisite of sustainability because, despite the potential for educational change that digitalization has offered since…

  17. Automated deadlock detection in synchronized reentrant multithreaded call-graphs

    NARCIS (Netherlands)

    I. Grabe (Immo); F.S. de Boer (Frank); J. van Leeuwen (Jan); A. Muscholl; D. Peleg; J. Pokorny; B. Rumpe

    2010-01-01

    textabstractIn this paper we investigate the synchronization of multithreaded call graphs with reentrance similar to call graphs in Java programs. We model the individual threads as Visibly Pushdown Automata (VPA) and analyse the reachability of a state in the product automaton by means of a Context

  18. Steven MacCall: Winner of LJ's 2010 Teaching Award

    Science.gov (United States)

    Berry, John N., III

    2010-01-01

    This article profiles Steven L. MacCall, winner of "Library Journal's" 2010 Teaching Award. An associate professor at the School of Library and Information Studies (SLIS) at the University of Alabama, Tuscaloosa, MacCall was nominated by Kathie Popadin, known as "Kpop" to the members of her cohort in the online MLIS program at SLIS. Sixteen of…

  19. 75 FR 31458 - Infrastructure Protection Data Call Survey

    Science.gov (United States)

    2010-06-03

    ... SECURITY National Protection and Programs Directorate Infrastructure Protection Data Call Survey AGENCY... Reduction Act of 1995 (Pub. L. 104-13, 44 U.S.C. Chapter 35). The National Protection and Programs... Protection Data Call Survey. Form: Not Applicable. OMB Number: 1670-NEW. Affected Public: Federal, State...

  20. Student Evaluation of CALL Tools during the Design Process

    Science.gov (United States)

    Nesbitt, Dallas

    2013-01-01

    This article discusses the comparative effectiveness of student input at different times during the design of CALL tools for learning kanji, the Japanese characters of Chinese origin. The CALL software "package" consisted of tools to facilitate the writing, reading and practising of kanji characters in context. A pre-design questionnaire…

  1. Effect of tracheosyringeal denervation on call in greenfinch (Carduelis sinica)

    Institute of Scientific and Technical Information of China (English)

    李东风; 李杰; 汪大伟; 蒋锦昌; 杨新宇; 陈浩

    1999-01-01

    After sections of left or right tracheosyringeal nerve (NXⅡts), greenfinches may repeat everyday calls, with no effect on temporal properties. It is suggested that either side of syrinx may produce sound alone and ipsilateral innervation of NXⅡts for the syringeal muscles. After section of left NXIIts, the bird produces the vocal pattern of partial tone increase, and effects on the sound intensity and sentence length average 1.4 and 2.8 times those after section of right NXIIts, suggesting that the innervation of NXIIts has left side dominance. After bilateral section of NXIIts, the call rhythm in company with expiratory motions is 98-146 times/min,on an average, and lose all sentence types and syllable structure of normal call. But the call spectra produced by tympaniform membrane vibrations without innervation still reserve frequency components similar to the tonic frequency and harmonics of normal calls.

  2. Evidence That Calls-Based and Mobility Networks Are Isomorphic.

    Directory of Open Access Journals (Sweden)

    Michele Coscia

    Full Text Available Social relations involve both face-to-face interaction as well as telecommunications. We can observe the geography of phone calls and of the mobility of cell phones in space. These two phenomena can be described as networks of connections between different points in space. We use a dataset that includes billions of phone calls made in Colombia during a six-month period. We draw the two networks and find that the call-based network resembles a higher order aggregation of the mobility network and that both are isomorphic except for a higher spatial decay coefficient of the mobility network relative to the call-based network: when we discount distance effects on the call connections with the same decay observed for mobility connections, the two networks are virtually indistinguishable.

  3. Investigating CALL in the Classroom: Situational Variables to Consider

    Directory of Open Access Journals (Sweden)

    Darlene Liutkus

    2012-01-01

    Full Text Available A new paradigm in second language pedagogy has Computer Assisted Language Learning (CALL playing a significant role. Much of the literature to-date claims that CALL can have a positive impact on students’ second language acquisition (SLA. Mixed method of research produces data to investigate if CALL positively affects student language proficiency, motivation and autonomy. Classroom observation of participants in their natural environment is a qualitative technique used but has situational variables that could skew results if not structured. A questionnaire is a quantitative tool that can offer insight regarding participants’ perception of performance but can contradict what the researcher has observed. This paper will take an in-depth look at variables such as: instructor’s pedagogical application; blending CALL into the curriculum; types of CALL implemented; feedback received and their implications for design of the data collection tools

  4. [Bioacoustic of the advertisement call of Ceratophrys cranwelli (Anura: Ceratophryidae)].

    Science.gov (United States)

    Valetti, Julián Alonso; Salas, Nancy Edith; Martino, Adolfo Ludovico

    2013-03-01

    The advertisement call plays an important role in the life history of anuran amphibians, mainly during the breeding season. Call features represent an important character to discriminate species, and sound emissions are very effective to assure or reinforce genetic incompatibility, especially in the case of sibling species. Since frogs are ectotherms, acoustic properties of their calls will vary with temperature. In this study, we described the advertisement call of C. cranwelli, quantifying the temperature effect on its components. The acoustic emissions were recorded during 2007 using a DAT record Sony TCD-100 with stereo microphone ECM-MS907 Sony and tape TDK DAT-RGX 60. As males emit their calls floating in temporary ponds, water temperatures were registered after recording the advertisement calls with a digital thermometer TES 1300+/-0.1 degreeC. Altogether, 54 calls from 18 males were analyzed. The temporal variables of each advertisement call were measured using oscillograms and sonograms and the analyses of dominant frequency were performed using a spectrogram. Multiple correlation analysis was used to identify the temperature-dependent acoustic variables and the temperature effect on these variables was quantified using linear regression models. The advertisement call of C. cranwelli consists of a single pulse group. Call duration, Pulse duration and Pulse interval decreased with the temperature, whereas the Pulse rate increased with temperature. The temperature-dependent variables were standardized at 25 degreeC according to the linear regression model obtained. The acoustic variables that were correlated with the temperature are the variables which emissions depend on laryngeal muscles and the temperature constraints the contractile properties of muscles. Our results indicated that temperature explains an important fraction of the variability in some acoustic variables (79% in the Pulse rate), and demonstrated the importance of considering the effect of

  5. Calling behavior of blue and fin whales off California

    Science.gov (United States)

    Oleson, Erin Marie

    Passive acoustic monitoring is an effective means for evaluating cetacean presence in remote regions and over long time periods, and may become an important component of cetacean abundance surveys. To use passive acoustic recordings for abundance estimation, an understanding of the behavioral ecology of cetacean calling is crucial. In this dissertation, I develop a better understanding of how blue (Balaenoptera musculus) and fin (B. physalus ) whales use sound with the goal of evaluating passive acoustic techniques for studying their populations. Both blue and fin whales produce several different call types, though the behavioral and environmental context of these calls have not been widely investigated. To better understand how calling is used by these whales off California I have employed both new technologies and traditional techniques, including acoustic recording tags, continuous long-term autonomous acoustic recordings, and simultaneous shipboard acoustic and visual surveys. The outcome of these investigations has led to several conclusions. The production of blue whale calls varies with sex, behavior, season, location, and time of day. Each blue whale call type has a distinct behavioral context, including a male-only bias in the production of song, a call type thought to function in reproduction, and the production of some calls by both sexes. Long-term acoustic records, when interpreted using all call types, provide a more accurate measure of the local seasonal presence of whales, and how they use the region annually, seasonally and daily. The relative occurrence of different call types may indicate prime foraging habitat and the presence of different segments of the population. The proportion of animals heard calling changes seasonally and geographically relative to the number seen, indicating the calibration of acoustic and visual surveys is complex and requires further study on the motivations behind call production and the behavior of calling whales

  6. Identification of sympatric bat species by the echolocation calls

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    One hundred and thirty-eight echolocation calls of 63 free-flying individuals of five bat species (Rhinolophus ferrumequinum,Myotis formosus,Myotis ikonnikovi,Myotis daubentoni and Murina leucogaster)were recorded (by ultrasonic bat detector (D980)) in Zhi'an village of Jilin Province,China.According to the frequency-time spectra,these calls were categorized into two types:FM/CF (constant frequency) / FM (R.ferrumequinum) and FM (frequency modulated)(M.formosus,M.ikonnikovi,M.daubentoni and M.leucogaster).Sonograms of the calls of R.ferrumequinum could easily be distinguished from those of the other four species.For the calls of the remaining four species,six echolocation call parameters,including starting frequency,ending frequency,peak frequency duration,longest inter-pulse interval and shortest inter-pulse interval,were examined by stepwise discriminant analysis.The results show that 84.1% of calls were correctly classified,which indicates that these parameters of echolocation calls play an important role in identifying bat species.These parameters can be used to test the accuracy of general predictions based on bats' morphology in the same forest and can provide essential information for assessing patterns of bat habitat use.

  7. Spectrographic Analysis of Carrion Crow Calls and Their Detection

    Science.gov (United States)

    Shibuya, Hisashi; Yokota, Yasunari

    In recent years, damage to agricultural products, livestock, and power transmission systems by crows is regarded as a serious problem; countermeasures against crow damage are urgently necessary. This paper proposed a method for detecting crow calls in various environmental sounds. If detection and discernment of crow calls were possible, various actions could be undertaken to prevent the damage. Wildlife call detection, not only that for crows, should be executed in extremely noisy environments. We then introduced both a spectrograph estimation technique with AR modeling in which AR coefficients are temporally smoothed and interpolated and a background noise elimination technique to obtain higher-quality crow call templates. The input sounds are compared with these templates by DP matching in the metric vector space of a logarithmic cepstrum. Every input sound whose minimal distance to the template database is less than the specified threshold value is detected as a crow call. The maximal detection performance can be obtained when five call templates in the template database are utilized; the implication is that carrion crows have five distinguishable call patterns. It is shown that the proposed method achieves 95% detection rate when 1.66% misdetection rate is allowed.

  8. The experience of work in a call centre environment

    Directory of Open Access Journals (Sweden)

    Sanet Hauptfleisch

    2006-02-01

    Full Text Available This qualitative research study explored the work experience in a call centre environment in an information technology call centre based in South Africa, which service foreign customers exclusively. Three data collection methods were used, namely narratives, in-depth interviews with call centre consultants, and observation. Following a grounded theory approach, four themes were elicited, namely the perceptions of team members, uncertainty created by a constantly changing environment, perceived distances due to management practices, and depersonalisation experienced while actually dealing with customers. In addition to this, the reported impact of these themes on work performance was explored and compared to existing research.

  9. Well Being and Job Satisfaction Among Call Centre Agents

    DEFF Research Database (Denmark)

    Wiegman, Inger Marie; Mathiesen, Karen; Møller, Niels

    2004-01-01

    A survey among 774 agents in four large Danish companies in house call centres shows that working in a call center is stressfull. Lack of control and autonomy, lack of potentiality and challenges, conflict between qualitative and quantitative demands and monitoring all have an adverse effect on job...... satisfaction. The survey reveals a need for improving working conditions and for development of the work as such in call centres. The survey suggests that the agents both want to participate in the process of development and that they can contribute to the process in a competent way. In the project as part...

  10. Well Being and Job Satisfaction Among Call Centre Agents

    DEFF Research Database (Denmark)

    Wiegman, Inger Marie; Mathiesen, Karen; Møller, Niels

    2004-01-01

    A survey among 774 agents in four large Danish companies in house call centres shows that working in a call center is stressfull. Lack of control and autonomy, lack of potentiality and challenges, conflict between qualitative and quantitative demands and monitoring all have an adverse effect on job...... satisfaction. The survey reveals a need for improving working conditions and for development of the work as such in call centres. The survey suggests that the agents both want to participate in the process of development and that they can contribute to the process in a competent way. In the project as part...

  11. Yoga Called Good Medicine for High Blood Pressure

    Science.gov (United States)

    ... fullstory_162446.html Yoga Called Good Medicine for High Blood Pressure People who added this practice to a healthy ... elevated blood pressure] are likely to develop hypertension [high blood pressure] unless they improve their lifestyle," said study author ...

  12. SNPMClust: Bivariate Gaussian Genotype Clustering and Calling for Illumina Microarrays

    Directory of Open Access Journals (Sweden)

    Stephen W. Erickson

    2016-07-01

    Full Text Available SNPMClust is an R package for genotype clustering and calling with Illumina microarrays. It was originally developed for studies using the GoldenGate custom genotyping platform but can be used with other Illumina platforms, including Infinium BeadChip. The algorithm first rescales the fluorescent signal intensity data, adds empirically derived pseudo-data to minor allele genotype clusters, then uses the package mclust for bivariate Gaussian model fitting. We compared the accuracy and sensitivity of SNPMClust to that of GenCall, Illumina's proprietary algorithm, on a data set of 94 whole-genome amplified buccal (cheek swab DNA samples. These samples were genotyped on a custom panel which included 1064 SNPs for which the true genotype was known with high confidence. SNPMClust produced uniformly lower false call rates over a wide range of overall call rates.

  13. Anuran Call Study Wertheim National Wildlife Refuge 1999

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — During May of 1999, the Wertheim National Wildlife Refuge participated in a nation - wide anuran call count study. This report outlines the results of that survey.

  14. Anuran Call Survey Summary 2002 Canaan Valley National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Anuran call surveys were conducted at the Canaan Valley National Wildlife Refuge for the first time in 2000 so this report summarizes the results of the refuge’s...

  15. The use of Computer Assisted Language Learning (CALL) Devices ...

    African Journals Online (AJOL)

    The use of Computer Assisted Language Learning (CALL) Devices in ... role of information and communication technologies in language learning and teaching. ... Classroom implications and applications for the effective Computer Assisted ...

  16. Anuran Call Survey Summary 2006 Canaan Valley National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Anuran call surveys were conducted at Canaan Valley National Wildlife Refuge in April-June 2006 using the protocol developed by the North American Amphibian...

  17. Trading Strategy Adipted Optimization of European Call Option

    OpenAIRE

    Fukumi, Toshio

    2005-01-01

    Optimal pricing of European call option is described by linear stochastic differential equation. Trading strategy given by a twin of stochastic variables was integrated w.r.t. Black-Scholes formula to adopt optimal pricing to tarading strategy.

  18. Anuran Call Survey Summary 2000 Canaan Valley National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Anuran call surveys were conducted at the Canaan Valley National Wildlife Refuge for the first time in 2000. Surveys for anurans are conducted in conjunction with...

  19. Family History of Colon Cancer Calls for Earlier Screening

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_164202.html Family History of Colon Cancer Calls for Earlier Screening ... 2017 (HealthDay News) -- If you've got a family history of colon or rectal cancers, you probably ...

  20. An Intelligent Call Admission Control Decision Mechanism for Wireless Networks

    CERN Document Server

    S., Ramesh Babu H; S, Satyanarayana P

    2010-01-01

    The Call admission control (CAC) is one of the Radio Resource Management (RRM) techniques plays instrumental role in ensuring the desired Quality of Service (QoS) to the users working on different applications which have diversified nature of QoS requirements. This paper proposes a fuzzy neural approach for call admission control in a multi class traffic based Next Generation Wireless Networks (NGWN). The proposed Fuzzy Neural Call Admission Control (FNCAC) scheme is an integrated CAC module that combines the linguistic control capabilities of the fuzzy logic controller and the learning capabilities of the neural networks .The model is based on Recurrent Radial Basis Function Networks (RRBFN) which have better learning and adaptability that can be used to develop the intelligent system to handle the incoming traffic in the heterogeneous network environment. The proposed FNCAC can achieve reduced call blocking probability keeping the resource utilisation at an optimal level. In the proposed algorithm we have c...

  1. Anuran Call Study Wertheim National Wildlife Refuge 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — For the second consecutive year, Wertheim National Wildlife Refuge participated in a nation-wide anuran call study. This report outlines the results of that survey...

  2. Formative Feedback in an Interactive Spoken CALL System

    OpenAIRE

    Tsourakis, Nikolaos; Rayner, Emmanuel; Baur, Claudia

    2014-01-01

    By definition spoken dialogue CALL systems should be easy to use and understand. However, interaction in this context is often far from unhindered. In this paper we introduce a formative feedback mechanism in our CALL system, which can monitor interaction, report errors and provide advice and suggestions to users. The distinctive feature of this mechanism is the ability to combine information from different sources and decide on the most pertinent feedback, which can also be adapted in terms ...

  3. The Call to Excellence--No Room for Complacency.

    Science.gov (United States)

    Mosher, Ruth

    2015-01-01

    The call of the nurse is to promote, protect, and support health in all areas of life. Christian nurses are further called to imitate Jesus Christ. Yet, complacency is a common attitude among nurses. With new national standards of collecting and publicly reporting patient perceptions of care, positive nursing attitudes and excellent care are imperative to successful outcomes. What does it mean to seek professional excellence?

  4. Processing of communication calls in Guinea pig auditory cortex.

    Directory of Open Access Journals (Sweden)

    Jasmine M S Grimsley

    Full Text Available Vocal communication is an important aspect of guinea pig behaviour and a large contributor to their acoustic environment. We postulated that some cortical areas have distinctive roles in processing conspecific calls. In order to test this hypothesis we presented exemplars from all ten of their main adult vocalizations to urethane anesthetised animals while recording from each of the eight areas of the auditory cortex. We demonstrate that the primary area (AI and three adjacent auditory belt areas contain many units that give isomorphic responses to vocalizations. These are the ventrorostral belt (VRB, the transitional belt area (T that is ventral to AI and the small area (area S that is rostral to AI. Area VRB has a denser representation of cells that are better at discriminating among calls by using either a rate code or a temporal code than any other area. Furthermore, 10% of VRB cells responded to communication calls but did not respond to stimuli such as clicks, broadband noise or pure tones. Area S has a sparse distribution of call responsive cells that showed excellent temporal locking, 31% of which selectively responded to a single call. AI responded well to all vocalizations and was much more responsive to vocalizations than the adjacent dorsocaudal core area. Areas VRB, AI and S contained units with the highest levels of mutual information about call stimuli. Area T also responded well to some calls but seems to be specialized for low sound levels. The two dorsal belt areas are comparatively unresponsive to vocalizations and contain little information about the calls. AI projects to areas S, VRB and T, so there may be both rostral and ventral pathways for processing vocalizations in the guinea pig.

  5. Non-linguistic analysis of call center conversations

    CERN Document Server

    Kopparapu, Sunil Kumar

    2014-01-01

    The book focuses on the part of the audio conversation not related to language such as speaking rate (in terms of number of syllables per unit time) and emotion centric features. This text examines using non-linguistics features to infer information from phone calls to call centers. The author analyzes 'how' the conversation happens and not 'what' the conversation is about by audio signal processing and analysis.

  6. La otra ciudad - Otros sujetos: los habitantes de la calle

    Directory of Open Access Journals (Sweden)

    Marta Elena Correa A.

    2007-01-01

    Full Text Available Este artículo está basado en el estudio "Caracterización demográfica, social, económica y familiar de los habitantes de calle de la comuna 11 de la ciudad de Medellín". En él se plantea la condición del habitante de calle como subproducto de concepciones

  7. Genotype and SNP calling from next-generation sequencing data

    DEFF Research Database (Denmark)

    Nielsen, Rasmus; Paul, Joshua S.; Albrechtsen, Anders

    2011-01-01

    Meaningful analysis of next-generation sequencing (NGS) data, which are produced extensively by genetics and genomics studies, relies crucially on the accurate calling of SNPs and genotypes. Recently developed statistical methods both improve and quantify the considerable uncertainty associated w...... with genotype calling, and will especially benefit the growing number of studies using low- to medium-coverage data. We review these methods and provide a guide for their use in NGS studies....

  8. Women Labour in Call Centres: Understanding Characteristics of Work

    Directory of Open Access Journals (Sweden)

    Gulten Dursun

    2014-07-01

    Full Text Available This paper is concerned with the work experiences of women employees in info-service-based offices as telephone call centres. Call centres have grown rapidly in Tur-key in recent years, creating a large number of new jobs. In particular, it is concerned with the question of whether call centre jobs are offering women new opportunities for career progression, or whether a more common bias is taking place in which women are being drawn into highly routinized jobs. The collection of data was carried out so-urcing a heterogeneous plurality of instruments. Our research confirms that work pro-cesses in call centres are close association of surveillance technologies (technologic panoptican, exploitation and high levels of discipline, highly repetitive and heavily mo-nitored, and that the association with the assembly line and Taylorism have dominated much of the rhetoric on call centres. In addition, we have observed that, the structure of women’s employment in the call centre industry tends to polarise.

  9. Social calls of flying big brown bats (Eptesicus fuscus

    Directory of Open Access Journals (Sweden)

    Genevieve Spanjer Wright

    2013-08-01

    Full Text Available Vocalizations serving a variety of social functions have been reported in many bat species (Order Chiroptera. While echolocation by big brown bats (Eptesicus fuscus has been the subject of extensive study, calls used by this species for communication have received comparatively little research attention. Here, we report on a rich repertoire of vocalizations produced by big brown bats in a large flight room equipped with synchronized high speed stereo video and audio recording equipment. Bats were studied individually and in pairs, while sex, age, and experience with a novel foraging task were varied. We used Discriminant Function Analysis to classify six different vocalizations that were recorded when two bats were present. Contingency table analyses revealed a higher prevalence of social calls when males were present, and some call types varied in frequency of emission based on trial type or bat age. Bats flew closer together around the time some social calls were emitted, indicating that communicative calls may be selectively produced when conspecifics fly near one another. These findings are the first reports of social calls from flying big brown bats and provide insight into the function of communicative vocalizations emitted by this species.

  10. Leveraging Call Center Logs for Customer Behavior Prediction

    Science.gov (United States)

    Parvathy, Anju G.; Vasudevan, Bintu G.; Kumar, Abhishek; Balakrishnan, Rajesh

    Most major businesses use business process outsourcing for performing a process or a part of a process including financial services like mortgage processing, loan origination, finance and accounting and transaction processing. Call centers are used for the purpose of receiving and transmitting a large volume of requests through outbound and inbound calls to customers on behalf of a business. In this paper we deal specifically with the call centers notes from banks. Banks as financial institutions provide loans to non-financial businesses and individuals. Their call centers act as the nuclei of their client service operations and log the transactions between the customer and the bank. This crucial conversation or information can be exploited for predicting a customer’s behavior which will in turn help these businesses to decide on the next action to be taken. Thus the banks save considerable time and effort in tracking delinquent customers to ensure minimum subsequent defaulters. Majority of the time the call center notes are very concise and brief and often the notes are misspelled and use many domain specific acronyms. In this paper we introduce a novel domain specific spelling correction algorithm which corrects the misspelled words in the call center logs to meaningful ones. We also discuss a procedure that builds the behavioral history sequences for the customers by categorizing the logs into one of the predefined behavioral states. We then describe a pattern based predictive algorithm that uses temporal behavioral patterns mined from these sequences to predict the customer’s next behavioral state.

  11. A Method for Calling Copy Number Polymorphism Using Haplotypes

    Directory of Open Access Journals (Sweden)

    Gun Ho eJang

    2013-09-01

    Full Text Available Single nucleotide polymorphism (SNP and copy number variation (CNV are both widespread characteristic of the human genome, but are often called separately on common genotyping platforms. To capture integrated SNP and CNV information, methods have been developed for calling allelic specific copy numbers or so called copy number polymorphism (CNP, using limited inter-marker correlation. In this paper, we proposed a haplotype-based maximum likelihood method to call CNP, which takes advantage of the valuable multi-locus linkage disequilibrium (LD information in the population. We also developed a computationally efficient EM algorithm to estimate haplotype frequencies and optimize individual CNP calls simultaneously, even at presence of missing data. Through simulations, we demonstrated our model is more sensitive and accurate in detecting various CNV regions, compared with commonly-used CNV calling methods including PennCNV, another hidden Markov model using CNP, a scan statistic, segCNV, and cnvHap. Our method often performs better in the regions with higher LD, in longer CNV regions, and in common CNV than the opposite. We implemented our method on the genotypes of 90 HapMap CEU samples and 23 patients with acute lung injury (ALI. For each ALI patient the genotyping was performed twice. The CNPs from our method show good consistency and accuracy comparable to others.

  12. Perceptual specificity in the alarm calls of Gunnison's prairie dogs.

    Science.gov (United States)

    Kiriazis, Judith; Slobodchikoff, C N

    2006-07-01

    Gunnison's prairie dogs have a complex alarm communication system. We show that the escape responses of prairie dogs to naturally occurring live predators differed depending upon the species of predator. We also show that playbacks of alarm calls that were elicited originally by the live predators produced the same escape responses as the live predators themselves. The escape responses fell into two qualitatively different categories: running to the burrow and diving inside for hawks and humans, and standing upright outside the burrow for coyotes and dogs. Within these two categories there were differences in response. For hawks, only the prairie dogs that were in the direct flight path of a stooping red-tailed hawk ran to their burrows and dove inside, while for humans and human alarm call playbacks there was a colony-wide running to the burrows and diving inside. For coyotes and coyote alarm call playbacks there was a colony-wide running to the burrows and standing alert at the burrow rims, while for domestic dogs and playbacks of alarm calls for domestic dogs the prairie dogs assumed an alert posture wherever they were feeding, but did not run to their burrows. These responses to both the live predators and to predator-elicited alarm calls suggest that the alarm calls of Gunnison's prairie dogs contain meaningful referential information about the categories of predators that approach a colony of prairie dogs.

  13. Single Time-Stamped Tries for Retroactive Call Subsumption

    CERN Document Server

    Cruz, Flavio

    2011-01-01

    Tabling is an evaluation strategy for Prolog programs that works by storing answers in a table space and then by using them in similar subgoals. Some tabling engines use call by subsumption, where it is determined that a subgoal will consume answers from a more general subgoal in order to reduce the search space and increase efficiency. We designed an extension, named Retroactive Call Subsumption (RCS), that implements call by subsumption independently of the call order, thus allowing a more general subgoal to force previous called subgoals to become answer consumers. For this extension, we propose a new table space design, the Single Time Stamped Trie (STST), that is organized to make answer sharing across subsumed/subsuming subgoals simple and efficient. In this paper, we present the new STST table space design and we discuss the main modifications made to the original Time Stamped Tries approach to non-retroactive call by subsumption. In experimental results, with programs that stress some deficiencies of ...

  14. Distress calls may honestly signal bird quality to predators.

    Science.gov (United States)

    Laiolo, Paola; Tella, José L; Carrete, Martina; Serrano, David; López, Guillermo

    2004-12-07

    In predator-prey interactions, both interactors may benefit from sharing information about prey vulnerability. We examined the relationship between calls used to discourage close predators (distress calls) and the health condition of the caller to test whether these signals are reliable indicators of prey quality. The structure of calls from captured lesser short-toed larks Calandrella rufescens was related to their body condition and T-cell-mediated immunocompetence. Birds in better nutritional and immunological condition utter harsher calls (i.e. they spread the call energy over a wider range of frequency) than birds in poorer conditions. Hence, the harshness of distress calls seems honestly to signal the health status of prey and thus their ability to escape, on which the predator might base its optimal foraging choice. Previous studies have investigated the honesty of songs that have evolved via sexual selection, but this is the first study, to our knowledge, the demonstrates a relationship between individual quality and a vocalization primarily shaped by natural selection.

  15. Responses of squirrel monkeys to their experimentally modified mobbing calls

    Science.gov (United States)

    Fichtel, Claudia; Hammerschmidt, Kurt

    2003-05-01

    Previous acoustic analyses suggested emotion-correlated changes in the acoustic structure of squirrel monkey (Saimiri sciureus) vocalizations. Specifically, calls given in aversive contexts were characterized by an upward shift in frequencies, often accompanied by an increase in amplitude. In order to test whether changes in frequencies or amplitude are indeed relevant for conspecific listeners, playback experiments were conducted in which either frequencies or amplitude of mobbing calls were modified. Latency and first orienting response were measured in playback experiments with six adult squirrel monkeys. After broadcasting yaps with increased frequencies or amplitude, squirrel monkeys showed a longer orienting response towards the speaker than after the corresponding control stimuli. Furthermore, after broadcasting yaps with decreased frequencies or amplitude, squirrel monkeys showed a shorter orienting response towards the speaker than after the corresponding manipulated calls with higher frequencies or amplitude. These results suggest that changes in frequencies or amplitude were perceived by squirrel monkeys, indicating that the relationship between call structure and the underlying affective state of the caller agreed with the listener's assessment of the calls. However, a simultaneous increase in frequencies and amplitude did not lead to an enhanced response, compared to each single parameter. Thus, from the receiver's perspective, both call parameters may mutually replace each other.

  16. Pulse register phonation in Diana monkey alarm calls

    Science.gov (United States)

    Riede, Tobias; Zuberbühler, Klaus

    2003-05-01

    The adult male Diana monkeys (Cercopithecus diana) produce predator-specific alarm calls in response to two of their predators, the crowned eagles and the leopards. The acoustic structure of these alarm calls is remarkable for a number of theoretical and empirical reasons. First, although pulsed phonation has been described in a variety of mammalian vocalizations, very little is known about the underlying production mechanism. Second, Diana monkey alarm calls are based almost exclusively on this vocal production mechanism to an extent that has never been documented in mammalian vocal behavior. Finally, the Diana monkeys' pulsed phonation strongly resembles the pulse register in human speech, where fundamental frequency is mainly controlled by subglottal pressure. Here, we report the results of a detailed acoustic analysis to investigate the production mechanism of Diana monkey alarm calls. Within calls, we found a positive correlation between the fundamental frequency and the pulse amplitude, suggesting that both humans and monkeys control fundamental frequency by subglottal pressure. While in humans pulsed phonation is usually considered pathological or artificial, male Diana monkeys rely exclusively on pulsed phonation, suggesting a functional adaptation. Moreover, we were unable to document any nonlinear phenomena, despite the fact that they occur frequently in the vocal repertoire of humans and nonhumans, further suggesting that the very robust Diana monkey pulse production mechanism has evolved for a particular functional purpose. We discuss the implications of these findings for the structural evolution of Diana monkey alarm calls and suggest that the restricted variability in fundamental frequency and robustness of the source signal gave rise to the formant patterns observed in Diana monkey alarm calls, used to convey predator information.

  17. Chronic intermittent ethanol exposure and withdrawal alters (3α,5α)-3-hydroxy-pregnan-20-one immunostaining in cortical and limbic brain regions of C57BL/6J mice.

    Science.gov (United States)

    Maldonado-Devincci, Antoniette M; Cook, Jason B; O'Buckley, Todd K; Morrow, Danielle H; McKinley, Raechel E; Lopez, Marcelo F; Becker, Howard C; Morrow, A Leslie

    2014-10-01

    The GABAergic neuroactive steroid (3α,5α)-3-hydroxy-pregnan-20-one (3α,5α-THP; allopregnanolone) has been studied during withdrawal from ethanol (EtOH) in humans, rats, and mice. Serum 3α,5α-THP levels decreased, and brain levels were not altered following acute EtOH administration (2 g/kg) in male C57BL/6J mice; however, the effects of chronic intermittent ethanol (CIE) exposure on 3α,5α-THP levels have not been examined. Given that CIE exposure changes subsequent voluntary EtOH drinking in a time-dependent fashion following repeated cycles of EtOH exposure, we conducted a time-course analysis of CIE effects on 3α,5α-THP levels in specific brain regions known to influence drinking behavior. Adult male C57BL/6J mice were exposed to 4 cycles of CIE to induce EtOH dependence. All mice were sacrificed and perfused at 1 of 2 time points, 8 or 72 hours following the final exposure cycle. Free-floating brain sections (40 μm; 3 to 5 sections/region/animal) were immunostained and analyzed to determine relative levels of cellular 3α,5α-THP. Withdrawal from CIE exposure produced time-dependent and region-specific effects on immunohistochemical detection of 3α,5α-THP levels across cortical and limbic brain regions. A transient reduction in 3α,5α-THP immunoreactivity was observed in the central nucleus of the amygdala 8 hours after withdrawal from CIE (-31.4 ± 9.3%). Decreases in 3α,5α-THP immunoreactivity were observed 72 hours following withdrawal in the medial prefrontal cortex (-25.0 ± 9.3%), nucleus accumbens core (-29.9 ± 6.6%), and dorsolateral striatum (-18.5 ± 6.0%), while an increase was observed in the CA3 pyramidal cell layer of the hippocampus (+42.8 ± 19.5%). Sustained reductions in 3α,5α-THP immunoreactivity were observed at both time points in the lateral amygdala (8 hours -28.3 ± 12.8%; 72 hours -27.5 ± 12.4%) and in the ventral tegmental area (8 hours -26.5 ± 9.9%; 72 hours -31.6 ± 13.8%). These data

  18. Referential alarm calling behaviour in New World primates

    Directory of Open Access Journals (Sweden)

    Cristiane CÄSAR, Klaus ZUBERBÜHLER

    2012-10-01

    Full Text Available There is relatively good evidence that non-human primates can communicate about objects and events in their environment in ways that allow recipients to draw inferences about the nature of the event experienced by the signaller. In some species, there is also evidence that the basic semantic units are not individual calls, but call sequences and the combinations generated by them. These two findings are relevant to theories pertaining to the origins of human language because of the resemblances of these phenomena with linguistic reference and syntactic organisation. Until recently, however, most research efforts on the primate origins of human language have involved Old World species with comparatively few systematic studies on New World monkeys, which has prevented insights into the deeper phylogenetic roots and evolutionary origins of language-relevant capacities. To address this, we review the older primate literature and very recent evidence for functionally referential communication and call combinations in New World primates. Within the existing literature there is ample evidence in both Callitrichids and Cebids for acoustically distinct call variants given to external disturbances that are accompanied by distinct behavioural responses. A general pattern is that one call type is typically produced in response to a wide range of general disturbances, often on the ground but also including inter-group encounters, while another call type is produced in response to a much narrower range of aerial threats. This pattern is already described for Old World monkeys and Prosimians, suggesting an early evolutionary origin. Second, recent work with black-fronted titi monkeys has produced evidence for different alarm call sequences consisting of acoustically distinct call types. These sequences appear to encode several aspects of the predation event simultaneously, notably predator type and location. Since meaningful call sequences have already been

  19. Referential alarm calling behaviour in New World primates

    Institute of Scientific and Technical Information of China (English)

    Cristiane C(A)SAR; Klaus ZUBERB(U)HLER

    2012-01-01

    There is relatively good evidence that non-human primates can communicate about objects and events in their environment in ways that allow recipients to draw inferences about the nature of the event experienced by the signaller.In some species,there is also evidence that the basic semantic units are not individual calls,but call sequences and the combinations generated by them.These two findings are relevant to theories pertaining to the origins of human language because of the resemblances of these phenomena with linguistic reference and syntactic organisation.Until recently,however,most research efforts on the primate origins of human language have involved Old Word species with comparatively few systematic studies on New World monkeys,which has prevented insights into the deeper phylogenetic roots and evolutionary origins of language-relevant capacities.To address this,we review the older primate literature and very recent evidence for functionally referential communication and call combinations in New World primates.Within the existing literature there is ample evidence in both Callitrichids and Cebids for acoustically distinct call variants given to external disturbances that are accompanied by distinct behavioural responses.A general pattern is that one call type is typically produced in response to a wide range of general disturbances,often on the ground but also including inter-group encounters,while another call type is produced in response to a much narrower range of aerial threats.This pattern is already described for Old World monkeys and Prosimians,suggesting an early evolutionary origin.Second,recent work with black-fronted titi monkeys has produced evidence for different alarm call sequences consisting of acoustically distinct call types.These sequences appear to encode several aspects of the predation event simultaneously,notably predator type and location.Since meaningful call sequences have already been described in Old Word primates,we suggest

  20. Call-related factors influencing output power from mobile phones.

    Science.gov (United States)

    Hillert, Lena; Ahlbom, Anders; Neasham, David; Feychting, Maria; Järup, Lars; Navin, Roshan; Elliott, Paul

    2006-11-01

    Mobile phone use is increasing but there is also concern for adverse health effects. Well-designed prospective studies to assess several health outcomes are required. In designing a study of mobile phone use, it is important to assess which factors need to be considered in classifying the exposure to radiofrequency fields (RF). A pilot study was performed in Sweden and in the UK 2002 to 2003 to test the feasibility of recruiting a cohort of mobile phone users from a random population sample and from mobile phone subscription lists for a prospective study. As one part of this pilot study, different factors were evaluated regarding possible influence on the output power of the phones. By local switch logging, information on calls made from predefined subscriptions or dedicated handsets were obtained and the output power of phones during calls made indoors and outdoors, in moving and stationary mode, and in rural as well in urban areas were compared. In this experiment, calls were either 1, 1.5 or 5 min long. The results showed that high mobile phone output power is more frequent in rural areas whereas the other factors (length of call, moving/stationary, indoor/outdoor) were of less importance. Urban and rural area should be considered in an exposure index for classification of the exposure to RF from mobile phones and may be assessed by first base station during mobile phone calls or, if this information is not available, possibly by using home address as a proxy.

  1. [A relational database to store Poison Centers calls].

    Science.gov (United States)

    Barelli, Alessandro; Biondi, Immacolata; Tafani, Chiara; Pellegrini, Aristide; Soave, Maurizio; Gaspari, Rita; Annetta, Maria Giuseppina

    2006-01-01

    Italian Poison Centers answer to approximately 100,000 calls per year. Potentially, this activity is a huge source of data for toxicovigilance and for syndromic surveillance. During the last decade, surveillance systems for early detection of outbreaks have drawn the attention of public health institutions due to the threat of terrorism and high-profile disease outbreaks. Poisoning surveillance needs the ongoing, systematic collection, analysis, interpretation, and dissemination of harmonised data about poisonings from all Poison Centers for use in public health action to reduce morbidity and mortality and to improve health. The entity-relationship model for a Poison Center relational database is extremely complex and not studied in detail. For this reason, not harmonised data collection happens among Italian Poison Centers. Entities are recognizable concepts, either concrete or abstract, such as patients and poisons, or events which have relevance to the database, such as calls. Connectivity and cardinality of relationships are complex as well. A one-to-many relationship exist between calls and patients: for one instance of entity calls, there are zero, one, or many instances of entity patients. At the same time, a one-to-many relationship exist between patients and poisons: for one instance of entity patients, there are zero, one, or many instances of entity poisons. This paper shows a relational model for a poison center database which allows the harmonised data collection of poison centers calls.

  2. Correlated evolution between hearing sensitivity and social calls in bats.

    Science.gov (United States)

    Bohn, Kirsten M; Moss, Cynthia F; Wilkinson, Gerald S

    2006-12-22

    Echolocating bats are auditory specialists, with exquisite hearing that spans several octaves. In the ultrasonic range, bat audiograms typically show highest sensitivity in the spectral region of their species-specific echolocation calls. Well-developed hearing in the audible range has been commonly attributed to a need to detect sounds produced by prey. However, bat pups often emit isolation calls with low-frequency components that facilitate mother-young reunions. In this study, we examine whether low-frequency hearing in bats exhibits correlated evolution with (i) body size; (ii) high-frequency hearing sensitivity or (iii) pup isolation call frequency. Using published audiograms, we found that low-frequency hearing sensitivity is not dependent on body size but is related to high-frequency hearing. After controlling for high-frequency hearing, we found that low-frequency hearing exhibits correlated evolution with isolation call frequency. We infer that detection and discrimination of isolation calls have favoured enhanced low-frequency hearing because accurate parental investment is critical: bats have low reproductive rates, non-volant altricial young and must often identify their pups within large crèches.

  3. Voice over internet protocol with prepaid calling card solutions

    Science.gov (United States)

    Gunadi, Tri

    2001-07-01

    The VoIP technology is growing up rapidly, it has big network impact on PT Telkom Indonesia, the bigger telecommunication operator in Indonesia. Telkom has adopted VoIP and one other technology, Intelligent Network (IN). We develop those technologies together in one service product, called Internet Prepaid Calling Card (IPCC). IPCC is becoming new breakthrough for the Indonesia telecommunication services especially on VoIP and Prepaid Calling Card solutions. Network architecture of Indonesia telecommunication consists of three layer, Local, Tandem and Trunck Exchange layer. Network development researches for IPCC architecture are focus on network overlay hierarchy, Internet and PSTN. With this design hierarchy the goal of Interworking PSTN, VoIP and IN calling card, become reality. Overlay design for IPCC is not on Trunck Exchange, this is the new architecture, these overlay on Tandem and Local Exchange, to make the faster call processing. The nodes added: Gateway (GW) and Card Management Center (CMC) The GW do interfacing between PSTN and Internet Network used ISDN-PRA and Ethernet. The other functions are making bridge on circuit (PSTN) with packet (VoIP) based and real time billing process. The CMC used for data storage, pin validation, report activation, tariff system, directory number and all the administration transaction. With two nodes added the IPCC service offered to the market.

  4. Improving of an Artificial Neural Networks Forecasting Model for Determining of the Number of Calls in 112 Emergency Call Center

    Directory of Open Access Journals (Sweden)

    Erdal Aydemir

    2014-05-01

    Full Text Available Forecasting studies are extremely important in the technical, social and economic research. Generally, we know it is very difficult to forecast with higher accurate about a system by using recent values. In the scientific literature, the forecasting studies of energy, personnel planning, production planning, climate changes, sales and marketing and economics etc. are frequently found. In this paper, for an emergency calls center in Isparta province of Turkey an artificial neural network (ANN forecasting model was developed to determine the number of calls for as health, fire and security services on a pilot implementation of the emergency calls center on a single number 112. In the developed model, the gradient descent with adaptive learning and momentum (GDX algorithm is selected as the training algorithm with feed-forward back-propagation by using 80% of input data and the 20% of input data is used for testing set data from last month. After the testing, the mean absolute percentage error (MAPE rate is obtained as 4.5% and it is useful to test. In addition, the forecasting results of the next month are shown that the MAPE values are 2.65%, 6.40% and 5.24% with ANN, trend analysis and ARIMA (1 1 1 models respectively and, the number of calls are found separately on the types of calls in daily. Consequently, the developed model by using ANN to forecast the number of calls in an emergency call center is more accurate than the trend analysis and ARIMA models.

  5. Why and when citizens call for emergency help: an observational study of 211,193 medical emergency calls.

    Science.gov (United States)

    Møller, Thea Palsgaard; Ersbøll, Annette Kjær; Tolstrup, Janne Schurmann; Østergaard, Doris; Viereck, Søren; Overton, Jerry; Folke, Fredrik; Lippert, Freddy

    2015-11-04

    A medical emergency call is citizens' access to pre-hospital emergency care and ambulance services. Emergency medical dispatchers are gatekeepers to provision of pre-hospital resources and possibly hospital admissions. We explored causes for access, emergency priority levels, and temporal variation within seasons, weekdays, and time of day for emergency calls to the emergency medical dispatch center in Copenhagen in a two-year study period (December 1(st), 2011 to November 30(th), 2013). Descriptive analysis was performed for causes for access and emergency priority levels. A Poisson regression model was used to calculate adjusted ratio estimates for the association between seasons, weekdays, and time of day overall and stratified by emergency priority levels. We analyzed 211,193 emergency calls for temporal variation. Of those, 167,635 calls were eligible for analysis of causes and emergency priority level. "Unclear problem" was the most frequent category (19%). The five most common causes with known origin were categorized as "Wounds, fractures, minor injuries" (13%), "Chest pain/heart disease" (11%), "Accidents" (9%), "Intoxication, poisoning, drug overdose" (8%), and "Breathing difficulties" (7%). The highest emergency priority levels (Emergency priority level A and B) were assigned in 81% of calls. In the analysis of temporal variation, the total number of calls peaked at wintertime (26%), Saturdays (16%), and during daytime (39%). The pattern of citizens' contact causes fell into four overall categories: unclear problems, medical problems, intoxication and accidents. The majority of calls were urgent. The magnitude of unclear problems represents a modifiable factor and highlights the potential for further improvement of supportive dispatch priority tools or educational interventions at dispatch centers. Temporal variation was identified within seasons, weekdays and time of day and reflects both system load and disease occurrence. Data on contact patterns

  6. Prototype for logging system calls and its overhead analysis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    With the capability of the virtual machine monitor,a novel approach for logging system activities is designed.In the design,the guest operating system runs on the virtual machine monitor as non-privileged mode.The redirecting and monitoring modules are added into the virtual machine monitor.When a guest application is calling a system call,it is trapped and redirected from the least privileged level into the virtual machine monitor running in the most privileged level.After logging is finished,it returns to the guest operating system running in the more privileged level and starts the system call.Compared with the traditional methods for logging system activities.the novel method makes it more difficult to destroy or tamper the logs.The preliminary evaluation shows that the prototype is simple and efficient.

  7. Intrusion detection based on system calls and homogeneous Markov chains

    Institute of Scientific and Technical Information of China (English)

    Tian Xinguang; Duan Miyi; Sun Chunlai; Li Wenfa

    2008-01-01

    A novel method for detecting anomalous program behavior is presented, which is applicable to hostbased intrusion detection systems that monitor system call activities. The method constructs a homogeneous Markov chain model to characterize the normal behavior of a privileged program, and associates the states of the Markov chain with the unique system calls in the training data. At the detection stage, the probabilities that the Markov chain model supports the system call sequences generated by the program are computed. A low probability indicates an anomalous sequence that may result from intrusive activities. Then a decision rule based on the number of anomalous sequences in a locality frame is adopted to classify the program's behavior. The method gives attention to both computational efficiency and detection accuracy, and is especially suitable for on-line detection. It has been applied to practical host-based intrusion detection systems.

  8. Characteristics of calls to the Israeli hotline during the Intifada.

    Science.gov (United States)

    Gilat, Itzhak; Latzer, Yael

    2007-08-01

    The present study examined the help-seeking characteristics of callers to the ten Israeli hotline centers during the Intifada - the Palestinian uprising in the Israeli administered territories. The research method combined quantitative and qualitative analyses of the volunteers' written reports. The quantitative analysis was conducted on a sample of 21,315 structured forms, and the qualitative content analysis was carried out on a sample of 498 verbal descriptions of calls. The quantitative analysis revealed a U-shaped curve illustrating the frequency of Intifada-related calls in relation to the time of the study. The qualitative analysis showed that the main complaints of the callers were focused on direct and masked manifestations of anxiety and feelings of helplessness. The implications of the findings are discussed in terms of understanding the unique psychological response to a new kind of stress, as seen from the perspective of calls to a hotline.

  9. Mental, physical and social health problems of call centre workers

    Directory of Open Access Journals (Sweden)

    P Bhuyar

    2008-01-01

    Full Text Available Background: Call centre workers in BPO face unique occupational hazards - mental, physical and psychosocial. Material & Method: A sample 100 call centre workers of both sexes and from two cities Pune and Mumbai were surveyed by both qualitative and quantitative methods for the above health problems. Results: A high proportion of workers faced sleep disturbances and associated mental stress and anxiety. Sleep disturbance and anxiety was significantly more in international call centres compared to domestic. There was also disturbance in circadian rhythms due to night shift. Physical problems such as musculoskeletal disorders, obesity, eye, and hearing problems were also present. Psychosocial problems included disruption in family life, use of tobacco and alcohol, and faulty eating habits. Conclusion: Better personal management, health education and more research is indicated to study the health problems in this emerging occupation.

  10. Complexity Theory and CALL Curriculum in Foreign Language Learning

    Directory of Open Access Journals (Sweden)

    Hassan Soleimani

    2014-05-01

    Full Text Available Complexity theory literally indicates the complexity of a system, behavior, or a process. Its connotative meaning, while, implies dynamism, openness, sensitivity to initial conditions and feedback, and adaptation properties of a system. Regarding English as a Foreign/ Second Language (EFL/ESL this theory emphasizes on the complexity of the process of teaching and learning, including all the properties of a complex system. The purpose of the current study is to discuss the role of CALL as a modern technology in simplifying the process of teaching and learning a new language while integrating into the complexity theory. Nonetheless, the findings obtained from reviewing previously conducted studies in this field confirmed the usefulness of CALL curriculum in EFL/ESL contexts. These findings can also provide pedagogical implications for employing computer as an effective teaching and learning tool. Keywords: Second/foreign language curriculum, CALL, Complexity theory

  11. Investigating Call Drops with Field Measurements on Commercial Mobile Phones

    DEFF Research Database (Denmark)

    Messina, Alessandro; Caragea, Gabriel; Compta, Pol Torres

    2013-01-01

    can be done per day. In this paper we present a new methodology to investigate call drops by using mobile phones to do the measurements following the concept of citizen sensing. Therefore, a mobile application for Android is made that collects all necessary data and dumps the measurement results......One of the biggest problems nowadays for network operators are occurring call drops. This problem has been increasing in the last years specially since the advent of 3G. The investigation in the operator's network is very time intensive and due to the highly priced hardware only a few measurements...... in a centralized database where the measurements are evaluated and represented on Google Maps. With a post analysis of the measurements, a classification of the call drops results is made. The collected data is also used to show some statistics related to the battery level and the received signal strength between...

  12. Why and when citizens call for emergency help

    DEFF Research Database (Denmark)

    Møller, Thea Palsgaard; Ersbøll, Annette Kjær; Tolstrup, Janne Schurmann

    2015-01-01

    BACKGROUND: A medical emergency call is citizens' access to pre-hospital emergency care and ambulance services. Emergency medical dispatchers are gatekeepers to provision of pre-hospital resources and possibly hospital admissions. We explored causes for access, emergency priority levels, and temp......, and ultimately development of best practice in the area of emergency medicine.......BACKGROUND: A medical emergency call is citizens' access to pre-hospital emergency care and ambulance services. Emergency medical dispatchers are gatekeepers to provision of pre-hospital resources and possibly hospital admissions. We explored causes for access, emergency priority levels...

  13. Informatics in Education and Koli Calling: a Comparative Analysis

    Directory of Open Access Journals (Sweden)

    SIMON

    2009-04-01

    Full Text Available The journal Informatics in Education and the conference Koli Calling are compared, starting with Simon's system for the classification of computing education papers and going on to conduct a brief bibliometric analysis of the authors of papers in both publications, including their repeat rates and the countries from which they come. The analysis finds that despite their different natures, the Lithuanian journal and the Finnish conference are highly comparable in many respects. The broad conclusion is that the two publications work well together - but it would be good to see some Lithuanian authors contributing papers to Koli Calling.

  14. Acoustic model optimisation for a call routing system

    CSIR Research Space (South Africa)

    Kleynhans, N

    2012-11-01

    Full Text Available . Through Dual Tone Multi-Frequency (DTMF) keypads and Automatic Speech Recognition (ASR), IVR sys- tems can capture digit information (such as account numbers) and more sophisticated information via a person?s speech (e.g. person?s name and surname... the versatility of an IVR system. A typical IVR application that makes use of speech pro- cessing capabilities is a call routing service, i.e. a system that routes incoming calls automatically to appropriate services or individuals. One such system...

  15. Executing application function calls in response to an interrupt

    Science.gov (United States)

    Almasi, Gheorghe; Archer, Charles J.; Giampapa, Mark E.; Gooding, Thomas M.; Heidelberger, Philip; Parker, Jeffrey J.

    2010-05-11

    Executing application function calls in response to an interrupt including creating a thread; receiving an interrupt having an interrupt type; determining whether a value of a semaphore represents that interrupts are disabled; if the value of the semaphore represents that interrupts are not disabled: calling, by the thread, one or more preconfigured functions in dependence upon the interrupt type of the interrupt; yielding the thread; and if the value of the semaphore represents that interrupts are disabled: setting the value of the semaphore to represent to a kernel that interrupts are hard-disabled; and hard-disabling interrupts at the kernel.

  16. Basket call option pricing for CCVG using sparse grids

    KAUST Repository

    Crocce, Fabian

    2016-01-06

    The use of processes with jumps to overcome the shortcomings of the classical Black and Scholes when modelling stock prices has became very popular. One of the best-known models is the Common Clock Variance Gamma model (CCVG), introduced by Madan and Seneta in the 1990 [3]. We propose a method to price European basket call options modelled by the CCVG. The method could be extended to other model obtained by the subordination of a multidimensional Brownian motion and to more general options. To simplify the expositions we consider calls under the CCVG.

  17. A Call-Graph Profiler for GNU Octave

    CERN Document Server

    Annamalai, Muthiah

    2008-01-01

    We report the design and implementation of a call-graph profiler for GNU Octave, a numerical computing platform. GNU Octave simplifies matrix computation for use in modeling or simulation. Our work provides a call-graph profiler, which is an improvement on the flat profiler. We elaborate design constraints of building a profiler for numerical computation, and benchmark the profiler by comparing it to the rudimentary timer start-stop (tic-toc) measurements, for a similar set of programs. The profiler code provides clean interfaces to internals of GNU Octave, for other (newer) profiling tools on GNU Octave.

  18. Defunctionalized Interpreters for Call-by-Need Evaluation

    DEFF Research Database (Denmark)

    Danvy, Olivier; Millikin, Kevin; Munk, Johan;

    2010-01-01

    Starting from the standard call-by-need reduction for the λ-calculus that is common to Ariola, Felleisen, Maraist, Odersky, and Wadler, we inter-derive a series of hygienic semantic artifacts: a reduction-free stateless abstract machine, a continuation-passing evaluation function, and what appears...... to be the first heapless natural semantics for call-by-need evaluation. Furthermore we observe that a data structure and a judgment in this natural semantics are in defunctionalized form. The refunctionalized counterpart of this evaluation function is an extended direct semantics in the sense of Cartwright...

  19. Mental and Physical health related problems of Call centre workers.

    Directory of Open Access Journals (Sweden)

    Dr. Vaibhavkumar Ramanuj

    2014-07-01

    Full Text Available Abstract: Background: The employees of a call centre in BPO may have certain occupational hazards which are unique for the industry. These may be mental, physical and psychosocial. Material & Method: A sample 100 call centre workers of both sexes and from day and night shifts were interviewed to find out about various health problems. Results: Mental stress was one of the main self reported health problem, reported by 44% and 54% of the respondents of day and night shift respectively. Sleep disturbance and disturbance in biological rhythm was mainly reported by the night shift workers.

  20. Jack London and "the Call of the Wild"

    Institute of Scientific and Technical Information of China (English)

    徐晶

    2002-01-01

    The article vividly analyzes the characteristics of Jack London's writing theme that is the description of the con-flicts between nature and human. Based on the "call of the wild" and its hero Buck, It points out the author' s writingthoughts, that is strong will human show in their fight against nature.

  1. Timing matters: sonar call groups facilitate target localization in bats.

    Science.gov (United States)

    Kothari, Ninad B; Wohlgemuth, Melville J; Hulgard, Katrine; Surlykke, Annemarie; Moss, Cynthia F

    2014-01-01

    To successfully negotiate a cluttered environment, an echolocating bat must control the timing of motor behaviors in response to dynamic sensory information. Here we detail the big brown bat's adaptive temporal control over sonar call production for tracking prey, moving predictably or unpredictably, under different experimental conditions. We studied the adaptive control of vocal-motor behaviors in free-flying big brown bats, Eptesicus fuscus, as they captured tethered and free-flying insects, in open and cluttered environments. We also studied adaptive sonar behavior in bats trained to track moving targets from a resting position. In each of these experiments, bats adjusted the features of their calls to separate target and clutter. Under many task conditions, flying bats produced prominent sonar sound groups identified as clusters of echolocation pulses with relatively stable intervals, surrounded by longer pulse intervals. In experiments where bats tracked approaching targets from a resting position, bats also produced sonar sound groups, and the prevalence of these sonar sound groups increased when motion of the target was unpredictable. We hypothesize that sonar sound groups produced during flight, and the sonar call doublets produced by a bat tracking a target from a resting position, help the animal resolve dynamic target location and represent the echo scene in greater detail. Collectively, our data reveal adaptive temporal control over sonar call production that allows the bat to negotiate a complex and dynamic environment.

  2. Phylogenetic relationships and call structure in four African bufonid ...

    African Journals Online (AJOL)

    1993-02-18

    Feb 18, 1993 ... rangeri and B. gutturalis, two species with very different call structures. are sister taxa. Two pairs ... species of Bulo, and then usc this phylogeny to infer the evolution of ...... D.G. 1984. The application of electrophoretic data in.

  3. Vascular malformation (so-called hemangioma) of Scarpa's ganglion

    NARCIS (Netherlands)

    Bernardeschi, D; Dunnebier, EA; Sauvaget, E; Herman, P; Wassef, M; Huy, PTB

    2004-01-01

    Vascular malformations of the cerebello-pontine angle (CPA) arising from the capillary plexus surrounding Scarpa's ganglion are rare tumors. We report a case of so-called "hemangioma" of the CPA which was operated on via a trans-labyrinthine approach based on a preoperative diagnosis of vestibular s

  4. 78 FR 19290 - Call for Nominations for Advisory Committees

    Science.gov (United States)

    2013-03-29

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Land Management Call for Nominations for Advisory Committees AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: The purpose of this notice is to reopen the request for...

  5. 75 FR 34487 - Sunshine Act Meetings (by Conference Call)

    Science.gov (United States)

    2010-06-17

    ... From the Federal Register Online via the Government Publishing Office NATIONAL COUNCIL ON DISABILITY Sunshine Act Meetings (by Conference Call) DATE AND TIMES: June 25, 2010, 1 p.m.-3 p.m. PLACE: NCD, 1331 F Street, NW., Suite 850, Washington, DC 20004. STATUS: Open to the public. MATTERS TO...

  6. Integrating CALL into an Iranian EAP Course: Constraints and Affordances

    Science.gov (United States)

    Mehran, Parisa; Alizadeh, Mehrasa

    2015-01-01

    Iranian universities have recently displayed a growing interest in integrating Computer-Assisted Language Learning (CALL) into teaching/learning English. The English for Academic Purposes (EAP) context, however, is not keeping pace with the current changes since EAP courses are strictly text-based and exam-oriented, and little research has thus…

  7. 76 FR 67747 - Interagency Autism Coordinating Committee; Call for Nominations

    Science.gov (United States)

    2011-11-02

    ... requirements and to form a balanced committee that represents the diversity within the community (details below... will serve as Special Government Employees. The Committee shall meet at the call of the chairperson or... these groups. The Department also encourages geographic diversity in the composition of the Committee...

  8. Echolocation Call Structure of Fourteen Bat Species in Korea

    Directory of Open Access Journals (Sweden)

    Fukui, Dai

    2015-07-01

    Full Text Available The echolocation calls of bats can provide useful information about species that are generally difficult to observe in the field. In many cases characteristics of call structure can be used to identify species and also to obtain information about aspects of the bat's ecology. We describe and compare the echolocation call structure of 14 of the 21 bat species found in Korea, for most of which the ecology and behavior are poorly understood. In total, 1,129 pulses were analyzed from 93 echolocation call sequences of 14 species. Analyzed pulses could be classified into three types according to the pulse shape: FM/CF/FM type, FM type and FM/QCF type. Pulse structures of all species were consistent with previous studies, although geographic variation may be indicated in some species. Overall classification rate provided by the canonical discriminant analysis was relatively low. Especially in the genera Myotis and Murina, there are large overlaps in spectral and temporal parameters between species. On the other hand, classification rates for the FM/QCF type species were relatively high. The results show that acoustic monitoring could be a powerful tool for assessing bat activity and distribution in Korea, at least for FM/QCF and FM/CF/FM species.

  9. Integrated Learning and Health: A Call for Increasing Coordination Efforts

    Science.gov (United States)

    Chafouleas, Sandra M.; Polifroni, E. Carol

    2016-01-01

    This commentary reinforces a call to recognize the need for substantive collaboration, communication, and cooperation across education and health sectors. Themes throughout the body of articles contained in the special issue are discussed as strengthening knowledge about the benefits to integration of education and health domains in prevention and…

  10. Rethinking Transfer: Learning from CALL Teacher Education as Consequential Transition

    Science.gov (United States)

    Chao, Chin-chi

    2015-01-01

    Behind CALL teacher education (CTE) there is an unproblematized consensus of transfer, which suggests a positivist and tool-centered view of learning gains that differs from the sociocultural focus of recent teacher education research. Drawing on Beach's (2003) conceptualization of transfer as "consequential transition," this…

  11. Physical Activity Promotion in Call Centres: Employers' Perspectives

    Science.gov (United States)

    Renton, Sheila J.; Lightfoot, Nancy E.; Maar, Marion A.

    2011-01-01

    This study followed a predominantly qualitative approach to explore the perspectives of employers in Sudbury, Ontario, Canada, call centres (CCs) regarding physical activity (PA) promotion in workplaces, by identifying current practices and employers' motivation to promote PA, as well as perceived facilitators and barriers. In-depth interviews…

  12. Musharraf calls for North-South cooperation: Science and technology

    CERN Multimedia

    2006-01-01

    "President Gen Pervez Musharraf has called for improved North-South cooperation in scinece and technology and said it would enable millions of people in the developing countries to realize the goal of rapid socio-economic progress" (1.5 page)

  13. The advertisement call of Ameerega pulchripecta (Silverstone, 1976)(Anura, Dendrobatidae).

    Science.gov (United States)

    Costa-Campos, Carlos Eduardo; Lima, Albertina Pimentel; Amézquita, Adolfo

    2016-07-06

    The name Ameerega picta was once used to denote a lineage of poison frogs (Dendrobatidae) distributed throughout most of the Amazon basin (Silverstone 1976); more recently, to describe a phenetic group involving at least 18 species, Lötters et al. (2007) pointed out that some of the lineages were indeed derived from the former A. picta. Among them, the nominal species with the widest distribution is A. hahneli (Haddad & Martins 1994; Twomey & Brown 2008), also an alleged complex of poorly defined species (Grant et al. 2006; Fouquet et al. 2007; Roberts et al. 2007). The mate-recognition signal, the advertisement call, was part of the evidence used to revalidate A. hahneli as a different species from A. picta. Although the advertisement call has been described for one or few individuals of other species in the group (Haddad & Martins 1994; Costa et al. 2006; Twomey & Brown 2008; Lötters et al. 2009), namely A. flavopicta, A. braccata and A. boehmei, and A. hahneli, we still lack a formal description for A. pulchripecta, the sister taxon of A. hahneli (Twomey & Brown 2008). Its call has been qualitatively described as similar to A. hahneli's call, but "deeper-voiced" (Lötters et al. 2007).

  14. Dream Catchers: "Margin Call," "Boss," and Climbing the Beanstalk

    Science.gov (United States)

    Beck, Bernard

    2012-01-01

    Two recent popular entertainments, "Margin Call" (Barnum, Dodson, Jenckes, Moosa, Quinto & Chandor, 2011) and "Boss" (Safinia, 2011), depict powerful and successful groups of bosses confronting sudden, extreme threats. They are forced to react quickly and sacrifice important values to achieve survival of their organizations. In these emergencies,…

  15. An ELT's Solution to Combat Plagiarism: "Birth" of CALL.

    Science.gov (United States)

    Sabieh, Christine

    One English-as-a Second-Language professor fought plagiarism using computer assisted language learning (CALL). She succeeded in getting half of her class to write documented research papers free of plagiarism. Although all of the students claimed to know how to avoid plagiarizing, 35 percent presented the work with minor traces of plagiarism. The…

  16. Inclusive Education National Research Advocacy Agenda: A Call to Action

    Science.gov (United States)

    Morningstar, Mary E.; Allcock, Heather C.; White, Julia M.; Taub, Deborah; Kurth, Jennifer A.; Gonsier-Gerdin, Jean; Ryndak, Diane L.; Sauer, Janet; Jorgensen, Cheryl M.

    2016-01-01

    The TASH Inclusive Education National Committee responded to Horner and Dunlap's call to ensure that future research integrates inclusive values with strong science by developing an inclusive education national research advocacy agenda. Qualitative methods were implemented to answer three questions: (a) "What is the state of inclusive…

  17. The Call to Vocation: Life Histories of Elderly Women Religious.

    Science.gov (United States)

    Wolf, Mary Alice

    1990-01-01

    Studied elderly religious women (n=30) using a phenomenological interview technique. Discusses women's "call to vocation," the remembered life events that influenced their decisions to enter the convent as young girls. Participants described motivations and spiritual direction. Uses remembrances of three women to demonstrate circumstances and…

  18. G-8 Leaders to Call for lighter Internet Regulation

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    @@ PARIS - Leaders of the Group of 8 industrialized countries are set to issue a provocative call for stron-ger Internet regulation, a cause championed by the host of the meeting, President Nicolas Sarkozy of France, but fiercely opposed by some Internet companies and free-speech groups.

  19. NPC and CPPCC Delegates Call for Release of Petroleum Law

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ Many delegates who attended the fifth session of Ninth National People's Congress and the fifth session of Ninth Chinese People's Political Consultative Conference called on the State to formulate "Petroleum and Natural Gas Law" as soon as possible for the purpose to operate petroleum and natural gas recovery, transportation and marketing on the legal basis.

  20. Orangutan call communication and the puzzle of speech evolution

    NARCIS (Netherlands)

    Reis E Lameira, A.

    2013-01-01

    Speech is a human hallmark. However, its evolution is little understood. It remains largely unknown which features of the call communication of our closest relatives – great apes – may have constituted speech evolutionary feedstock. In this study, I investigate the extent to which speech building bl

  1. 47 CFR 64.605 - Emergency calling requirements.

    Science.gov (United States)

    2010-10-01

    ... providers of VRS and IP Relay to which § 64.605(b) applies. (2) Each provider of Internet-based TRS shall... voice telephone call between the CA and the PSAP, designated statewide default answering point, or... VRS and IP Relay—(1) Scope. The following requirements are only applicable to providers of VRS or IP...

  2. Wild birds learn to eavesdrop on heterospecific alarm calls.

    Science.gov (United States)

    Magrath, Robert D; Haff, Tonya M; McLachlan, Jessica R; Igic, Branislav

    2015-08-01

    Many vertebrates gain critical information about danger by eavesdropping on other species' alarm calls [1], providing an excellent context in which to study information flow among species in animal communities [2-4]. A fundamental but unresolved question is how individuals recognize other species' alarm calls. Although individuals respond to heterospecific calls that are acoustically similar to their own, alarms vary greatly among species, and eavesdropping probably also requires learning [1]. Surprisingly, however, we lack studies demonstrating such learning. Here, we show experimentally that individual wild superb fairy-wrens, Malurus cyaneus, can learn to recognize previously unfamiliar alarm calls. We trained individuals by broadcasting unfamiliar sounds while simultaneously presenting gliding predatory birds. Fairy-wrens in the experiment originally ignored these sounds, but most fled in response to the sounds after two days' training. The learned response was not due to increased responsiveness in general or to sensitization following repeated exposure and was independent of sound structure. Learning can therefore help explain the taxonomic diversity of eavesdropping and the refining of behavior to suit the local community. In combination with previous work on unfamiliar predator recognition (e.g., [5]), our results imply rapid spread of anti-predator behavior within wild populations and suggest methods for training captive-bred animals before release into the wild [6]. A remaining challenge is to assess the importance and consequences of direct association of unfamiliar sounds with predators, compared with social learning-such as associating unfamiliar sounds with conspecific alarms.

  3. School Social Workers and a Renewed Call to Advocacy

    Science.gov (United States)

    Joseph, Alfred L., Jr.; Slovak, Karen; Broussard, C. Anne

    2010-01-01

    This article calls for school social workers to work in schools to reform school systems that have historically failed and are currently failing African American children. While the hope of education is to assist students to realize their potential, school systems across the nation are not reaching this goal. School social work has a duty, a…

  4. 78 FR 31977 - NASA Asteroid Initiative Call for Ideas

    Science.gov (United States)

    2013-05-28

    ... SPACE ADMINISTRATION NASA Asteroid Initiative Call for Ideas AGENCY: National Aeronautics and Space... announces a public forum to provide a status on the agency's asteroid initiative planning and to encourage...: This meeting will be streamed live online. Viewing options will be posted at www.nasa.gov/asteroid...

  5. Vascular malformation (so-called hemangioma) of Scarpa's ganglion

    NARCIS (Netherlands)

    Bernardeschi, D; Dunnebier, EA; Sauvaget, E; Herman, P; Wassef, M; Huy, PTB

    2004-01-01

    Vascular malformations of the cerebello-pontine angle (CPA) arising from the capillary plexus surrounding Scarpa's ganglion are rare tumors. We report a case of so-called "hemangioma" of the CPA which was operated on via a trans-labyrinthine approach based on a preoperative diagnosis of vestibular

  6. 76 FR 44333 - National Biodefense Science Board; Call for Nominees

    Science.gov (United States)

    2011-07-25

    ..., biotechnology, and genetic engineering with respect to threats posed by naturally occurring infectious diseases... HUMAN SERVICES National Biodefense Science Board; Call for Nominees AGENCY: Office of the Secretary, Department of Health and Human Services. ACTION: Notice. SUMMARY: The Office of the Secretary is...

  7. Physical Activity Promotion in Call Centres: Employers' Perspectives

    Science.gov (United States)

    Renton, Sheila J.; Lightfoot, Nancy E.; Maar, Marion A.

    2011-01-01

    This study followed a predominantly qualitative approach to explore the perspectives of employers in Sudbury, Ontario, Canada, call centres (CCs) regarding physical activity (PA) promotion in workplaces, by identifying current practices and employers' motivation to promote PA, as well as perceived facilitators and barriers. In-depth interviews…

  8. Dream Catchers: "Margin Call," "Boss," and Climbing the Beanstalk

    Science.gov (United States)

    Beck, Bernard

    2012-01-01

    Two recent popular entertainments, "Margin Call" (Barnum, Dodson, Jenckes, Moosa, Quinto & Chandor, 2011) and "Boss" (Safinia, 2011), depict powerful and successful groups of bosses confronting sudden, extreme threats. They are forced to react quickly and sacrifice important values to achieve survival of their organizations. In these emergencies,…

  9. Development of the Crisis Call Outcome Rating Scale.

    Science.gov (United States)

    Bonneson, Mary E.; Hartsough, Don M.

    1987-01-01

    Constructed and tested Crisis Call Outcome Rating Scale, rating scale of caller behavior, as an outcome measure at telephone hotline facility. Psychology students rated caller behavior on role-played audiotapes. Items having respectable item-total correlations comprise final version of scale which detected meaningful differences among audiotapes.…

  10. 78 FR 4369 - Rates for Interstate Inmate Calling Services

    Science.gov (United States)

    2013-01-22

    ... pros or cons of debit calling? We seek comment on ICS providers' overall experiences with offering... technologies by ICS providers impacts our analysis under section 276 of the Act. To what extent are providers currently utilizing VoIP technology to provide ICS? Would the use of VoIP technology affect the authority...

  11. [Emergency ambulance call-outs often provide primary care

    NARCIS (Netherlands)

    Smits, M.; Francissen, O.; Weerts, M.; Janssen, K.; Grunsven, P. van; Giesen, P.

    2014-01-01

    OBJECTIVE: To examine patient and care characteristics of emergency ambulance call-outs and to determine how many of them were, in retrospect, effectively providing primary care. DESIGN: Retrospective cross-sectional study. METHOD: We charted patient and care characteristics of 598 emergency

  12. Probabilistic Anomaly Detection Based On System Calls Analysis

    Directory of Open Access Journals (Sweden)

    Przemysław Maciołek

    2007-01-01

    Full Text Available We present an application of probabilistic approach to the anomaly detection (PAD. Byanalyzing selected system calls (and their arguments, the chosen applications are monitoredin the Linux environment. This allows us to estimate “(abnormality” of their behavior (bycomparison to previously collected profiles. We’ve attached results of threat detection ina typical computer environment.

  13. A Call for Change: Providing Solutions for Black Male Achievement

    Science.gov (United States)

    Casserly, Michael; Lewis, Sharon; Simon, Candace; Uzzell, Renata; Palacios, Moses

    2012-01-01

    In October 2010, the Council of the Great City Schools released a major report on the academic status of African American males, "A Call for Change: The Social and Educational Factors Contributing to the Outcomes of Black Males in Urban Schools." The report was the first phase of the Council's efforts to recommit the energies of the nation's urban…

  14. Information technology - Telecommunications and information exchange between systems - Private integrated services network - Specification, functional model and information flows - Call priority interruption and call priority interruption protection supplementary services

    CERN Document Server

    International Organization for Standardization. Geneva

    2003-01-01

    Information technology - Telecommunications and information exchange between systems - Private integrated services network - Specification, functional model and information flows - Call priority interruption and call priority interruption protection supplementary services

  15. Information technology - Telecommunications and information exchange between systems - Private integrated services network - Inter-exchange signalling protocol - Call priority interruption and call priority interruption protection supplementary services

    CERN Document Server

    International Organization for Standardization. Geneva

    2003-01-01

    Information technology - Telecommunications and information exchange between systems - Private integrated services network - Inter-exchange signalling protocol - Call priority interruption and call priority interruption protection supplementary services

  16. Assessment of the noise exposure of call centre operators.

    Science.gov (United States)

    Patel, Jacqueline A; Broughton, Keith

    2002-11-01

    Call centres now play a major role in the daily operations of financial, technology and utility companies, as well as public bodies. It is predicted that 2002 will see 2.3% of the total British workforce employed in call centres. However, local authority enforcement officers, unions, voluntary organizations, employers and employees have all expressed concern that there are hazards to health and safety unique to this new and developing industry. One of the potential hazards reported in the press is hearing damage from using headsets. In a Health & Safety Executive funded project, the noise exposure of 150 call centre operators was evaluated, in call centres which included financial services, home shopping and telecommunications services. The results show that the daily personal noise exposure of these call centre operators is unlikely to exceed the 85 dB(A) action level defined in the Noise at Work Regulations 1989. The risk of hearing damage is therefore extremely low. Exposure to higher noise levels is possible, for example from fax tones, holding tones and high pitched tones from mobile phones. However, the duration of these events is likely to be short and they are therefore unlikely to have a significant effect on the operators' overall noise exposure. A practical method of limiting exposure to unexpected high noises from headsets is to ensure that the headsets incorporate acoustic shock protection that meets the requirements of the Department of Trade and Industry specification 85/013. In the UK, this limiter ensures any noise above 118 dB is not transmitted through the headset. Operators should receive regular training on the headset and telephone equipment they are using. This training should include correct use of the headset and the volume control facilities, and advice on how and when to clean and maintain the headsets.

  17. Superior Ambulance Call Out Rate Forecasting Using Meteorological Data

    Science.gov (United States)

    Mahmood, M. A.; Thornes, J. E.; Bloss, W.; Pope, F.

    2015-12-01

    Ambulances are an integral part of a country's infrastructure ensuring its citizens and visitors are kept healthy. The impact of weather, climate and climate change on ambulance services around the world has received increasing attention in recent years but most studies have been area specific and there is a need to establish basic relationships between ambulance data (both response and illness data) and meteorological parameters. In this presentation, the effects of temperature and relative humidity on ambulance call out rates for different medical categories will be investigated. We use call out data obtained from the London Ambulance Service (LAS) and meteorological data from a central London meteorological station. A time-series analysis was utilized to understand the relation between temperature, relative humidity, air pollutants and different call out categories. There are statistically significant relationships between mean temperature and ambulance callout rate for most of the categories investigated. Most categories show a negative dependence on temperature, i.e. call outs increase with decreasing temperature but some categories showed a positive dependence such as alcohol related call outs. Relative humidity is significant for some categories but in general is much less important than temperature. Significant time lag effects were observed for most of the categories related to infectious illnesses, which are transferrable through human contact. These findings support the opinion that ambulance attendance callouts records are an effective and well-timed source of data and can be used for health early warning systems. Furthermore the presented results can much improve our understanding of the relationships between meteorological conditions and human health thereby allowing for better prediction of ambulance use through the application of long and short-term weather forecasts.

  18. A Program Transformation for Continuation Call-Based Tabled Execution

    CERN Document Server

    de Guzman, Pablo Chico; Hermenegildo, Manuel V

    2009-01-01

    The advantages of tabled evaluation regarding program termination and reduction of complexity are well known --as are the significant implementation, portability, and maintenance efforts that some proposals (especially those based on suspension) require. This implementation effort is reduced by program transformation-based continuation call techniques, at some efficiency cost. However, the traditional formulation of this proposal by Ramesh and Cheng limits the interleaving of tabled and non-tabled predicates and thus cannot be used as-is for arbitrary programs. In this paper we present a complete translation for the continuation call technique which, using the runtime support needed for the traditional proposal, solves these problems and makes it possible to execute arbitrary tabled programs. We present performance results which show that CCall offers a useful tradeoff that can be competitive with state-of-the-art implementations.

  19. The World Health Organization "Rehabilitation 2030: a call for action".

    Science.gov (United States)

    Gimigliano, Francesca; Negrini, Stefano

    2017-04-01

    February 6th-7th, 2017 might become a memorable date in the future of rehabilitation. On these two days, the World Health Organization (WHO) has summoned over 200 stakeholders in the Executive Board Room of the WHO Headquarters in Geneva, Switzerland. Their common aim was to a launch the "Rehabilitation 2030" call to action and to present the WHO Recommendations on rehabilitation in health systems. These initiatives are meant to draw attention to the increasing unmet need for rehabilitation in the world; to highlight the role of rehabilitation in achieving the Sustainable Development Goals proposed by the United Nations; to call for coordinated and concerted global action towards strengthening rehabilitation in health systems. The aim of this paper is to report on the scientific events of these 2 days, which will most likely mark the history of rehabilitation.

  20. The Attitudes of The Students in Sofl Towards Call

    Directory of Open Access Journals (Sweden)

    Yasin ASLAN

    2011-06-01

    Full Text Available With the advent of multimedia computing and the Internet, the role of computers in language instruction has now become an important issue confronting large numbers of language teachers throughout the world. Computer Assisted Language Learning (CALL facilities and internet resources help English teachers to search lonely to get information in a stable and comfortable manner. Besides, at educational areas, students who start learning English in an intensive program in School of Foreign Languages get various capacities and properties for their future life. In that sense, technology makes people to have self-differentiated features in order to get wide range of knowledge. This study points out the attitudes of students, who attend School of Foreign Languages, with regard to CALL by considering their tendencies emerging with described statements to realize new generation perspectives. The questionnaire applied to 171 students in SOFL at Selçuk University examined the consciousness of students towards internet.

  1. Dimensionality of organizational justice in a call center context.

    Science.gov (United States)

    Flint, Douglas; Haley, Lynn M; McNally, Jeffrey J

    2012-04-01

    Summary.-Employees in three call centers were surveyed about their perceptions of organizational justice. Four factors were measured: distributive justice, procedural justice, interpersonal justice, and informational justice. Structural equation modeling was employed to test whether a two-, three-, or four-factor model best fit the call center data. A three-factor model of distributive, procedural, and informational justice provided the best fit to these data. The three-factor model that showed the best fit does not conform to any of the more traditional models identified in the organizational justice literature. This implies that the context in which organizational justice is measured may play a role in identifying which justice factors are relevant to employees. Findings add to the empirical evidence on the dimensionality of organizational justice and imply that dimensionality of organizational justice is more context-dependent than previously thought.

  2. Objective Assessment of IP Video Calls with Asterisk

    Directory of Open Access Journals (Sweden)

    Lukas Kapicak

    2012-01-01

    Full Text Available The paper deals with an objective assessment of IP video calls transmission over GSM and UMTS networks. Video transmission is affected by many factors in mobile network. Among these factors belong packet loss, latency and transmission rate of the mobile network. Network properties were simulated by Simena network simulator. Our team have developed a unique technique for finding defects in video appearing in video calls. This technique is built on modified Asterisk SW PBX with enabled video recording and playback functions. Transmitted video files are compared with original video file by means of size of transmitted video file and in-video-defects. We are using MSU VQMT software for finding in video defects; more precisely we are using VQM method for comparing two video sources.

  3. Estonian Leader's Freedom Call Creates Storm / Anna Smolchenko

    Index Scriptorium Estoniae

    Smolchenko, Anna

    2008-01-01

    President Toomas Hendrik Ilvese esinemisest soome-ugri rahvaste maailmakongressil Hantõ-Mansiiskis, kohtumisest Venemaa presidendi Dmitri Medvedeviga, Eesti delegatsiooni saalist väljamarssimisest Venemaa riigiduuma väliskomisjoni esimehe Konstantin Kossatshovi sõnavõtu ajal. Ilmunud ka: St. Petersburg Times 1. juuli 2008, pealk.: Estonian Leader's Freedom Call Creates Controversy (lüh.). Vabariigi President töövisiidil Venemaal 27.-30.06.2008

  4. New AIDA-2020 call for breakthrough detector technologies

    CERN Multimedia

    2016-01-01

    Physicists, engineers, and industry will be interested in a new proof-of-concept fund for breakthrough projects from the general field of detector development and testing.   Launched in the framework of the European project AIDA-2020, this open call will provide up to 200k€ of seed funding to support innovative and societal applications with a focus on industry-oriented applications. The deadline for applying is 20 October 2016. More information here.

  5. Anomaly-Based Intrusion Detection Systems Utilizing System Call Data

    Science.gov (United States)

    2012-03-01

    Functionality Description Persistence mechanism Mimicry technique Camouflage malware image: • renaming its image • appending its image to victim... Age ”, ACSAC 2007 21. Chien Eric, “Techniques of Adware and Spyware”, White paper: Symantec Security Response, in proceedings of VB2005, Dublin...Johnson R. “A practical mimicry attack against powerful system-call monitors” In Proc. ACM Symposium on Information, Computer and Communications

  6. Estonian Leader's Freedom Call Creates Storm / Anna Smolchenko

    Index Scriptorium Estoniae

    Smolchenko, Anna

    2008-01-01

    President Toomas Hendrik Ilvese esinemisest soome-ugri rahvaste maailmakongressil Hantõ-Mansiiskis, kohtumisest Venemaa presidendi Dmitri Medvedeviga, Eesti delegatsiooni saalist väljamarssimisest Venemaa riigiduuma väliskomisjoni esimehe Konstantin Kossatshovi sõnavõtu ajal. Ilmunud ka: St. Petersburg Times 1. juuli 2008, pealk.: Estonian Leader's Freedom Call Creates Controversy (lüh.). Vabariigi President töövisiidil Venemaal 27.-30.06.2008

  7. Leveraging management information in improving call centre productivity

    OpenAIRE

    Manthisana Mosese; Martie Mearns

    2016-01-01

    Background: The availability and efficient use of management information is one of the key strategic levers in driving growth and competitiveness for companies. Management information facilitates vital decision making that assists organisations in improving their competitiveness. For call centre operations, competitiveness entails improving productivity and customer service, and management information is essential in this endeavour.Objectives: This research explored the use of management info...

  8. Psychopharmacology training in clinical psychology: a renewed call for action.

    Science.gov (United States)

    Julien, Robert M

    2011-04-01

    Knowledge of psychopharmacology is essential for a clinical psychologist to practice his/her profession, regardless of whether one desires to become licensed to prescribe psychoactive medications. This commentary reiterates a call made almost 20 years ago for all practitioners to gain and utilize this knowledge. Without psychopharmacology knowledge, one is extremely limited in the ability to interact with medical prescribers and to optimally serve their patients as a valued member of the health care team.

  9. A Histopathological Study of the so-called Buruli ulcer

    OpenAIRE

    山下, 裕人; 千馬, 正敬; 板倉, 英世

    1980-01-01

    A histopathological study of the so-called "Buruli ulcer" was reported. The patient was a 10-year-old African boy in Kenya suffering from ulcer formation. The ulcer was 10cm in diameter and located over the left hip. It was undermined and covered by overhanged epithelia at the ulcer edge. There was edema or lytic change in the dermis which formed gaps between the epidermis and dermis in adjacent regions. Microscopically, inflammatory cells infiltration, granulation tissue, fibrosis, and sever...

  10. CEE/CA: Report calls for decriminalization of sex work.

    Science.gov (United States)

    Betteridge, Glenn

    2006-04-01

    In December 2005, the Central and Eastern European Harm Reduction Network (CEEHRN) released a report calling for the decriminalization of sex work in the 27 countries of Central and Eastern Europe and Central Asia (CEE/CA). The report brings together a wealth of published and original information concerning sex work, laws regulating sex work, epidemiological data regarding HIV and other sexually transmitted infections (STIs), services available to sex workers, and human rights abuses faced by sex workers.

  11. Towards Agent-Oriented Approach to a Call Management System

    Science.gov (United States)

    Ashamalla, Amir Nabil; Beydoun, Ghassan; Low, Graham

    There is more chance of a completed sale if the end customers and relationship managers are suitably matched. This in turn can reduce the number of calls made by a call centre reducing operational costs such as working time and phone bills. This chapter is part of ongoing research aimed at helping a CMC to make better use of its personnel and equipment while maximizing the value of the service it offers to its client companies and end customers. This is accomplished by ensuring the optimal use of resources with appropriate real-time scheduling and load balancing and matching the end customers to appropriate relationship managers. In a globalized market, this may mean taking into account the cultural environment of the customer, as well as the appropriate profile and/or skill of the relationship manager to communicate effectively with the end customer. The chapter evaluates the suitability of a MAS to a call management system and illustrates the requirement analysis phase using i* models.

  12. Prazer e sofrimento entre os trabalhadores de Call Center

    Directory of Open Access Journals (Sweden)

    Carolina Scolari

    2009-12-01

    Full Text Available Os conceitos de Taylor foram fundamentais para o desenvolvimento industrial no século XX. Ainda hoje, seus ensinamentos estão presentes em diversos ramos da economia, inclusive, no setor de call center. Call center é uma estrutura que visa ao atendimento de clientes com a utilização de um terminal de computador e de um aparelho telefônico. Esse setor sofreu grande expansão e, em 2006, foi responsável pela geração de 60 mil empregos diretos, tornando-se uma forma de contato e negócios atrativa para empresas do ramo público e privado. O objetivo deste artigo foi analisar as vivências de prazer e sofrimento no trabalho entre os operadores de call centers. Foi realizada uma pesquisa qualitativa, estratégia de estudo de caso e método de análise dos dados, análise de conteúdo. Como resultados, foram elaboradas algumas categorias finais, entre elas as mediações no trabalho, analisada no presente artigo sob o referencial teórico da psicodinâmica do trabalho.

  13. Application of a smartphone nurse call system for nursing care.

    Science.gov (United States)

    Chuang, Shu-Ting; Liu, Yi-Fang; Fu, Zi-Xuan; Liu, Kuang-Chung; Chien, Sou-Hsin; Lin, Chin-Lon; Lin, Pi-Yu

    2015-02-01

    Traditionally, a patient presses the nurse call button and alerts the central nursing station. This system cannot reach the primary care nurse directly. The aim of this study was to apply a new smartphone system through the cloud system and information technology that linked a smartphone and a mobile nursing station for nursing care service. A smartphone and mobile nursing station were integrated into a smartphone nurse call system through the cloud and information technology for better nursing care. Waiting time for a patient to contact the most responsible nurse was reduced from 3.8 min to 6 s. The average time for pharmacists to locate the nurse for medication problem was reduced from 4.2 min to 1.8 min by the new system. After implementation of the smartphone nurse call system, patients received a more rapid response. This improved patients' satisfaction and reduced the number of complaints about longer waiting time due to the shortage of nurses.

  14. Report Calls for National Commitment to Protect Oceans

    Science.gov (United States)

    Showstack, Randy

    A widely-anticipated report on the ecological health of ocean waters and coastal areas of the United States calls for extensive restoration and greatly improved protection of these resources. The report of the independent, non-partisan Pew Oceans Commission, released on 4 June, specifically calls for wholesale reforms in ocean governance, in fisheries and coastal management, and in federal laws and regulations pertaining to ocean and coastal pollution. It also calls for a doubling of the amount of money that the federal government spends on ocean-related research. The result of three years of research on ocean and coastal resources issues, the report declares that ``America's oceans are in a crisis'' from pollution, unwise and overuse of some resources, the pressures of human population on coastal areas, and other problems. It identifies the root cause, however, as ``a failure of both perspective and governance,'' in which regulation and management are approached with an out-of-date and non-unified set of laws and programs that were made ``on a crisis-by-crisis, sector-by-sector basis.''

  15. INTER-BANK CALL MONEY MARKET TRANSACTION IN INDONESIA

    Directory of Open Access Journals (Sweden)

    Aries Haryadi

    2013-10-01

    Full Text Available AbstractThis study analyzes the effect of Indonesian Bank loan, the amount of demand deposits, and the Indonesia economic crisis in 1997 on the interbank call money market transactions in Indonesia using a multiple linear regression method. This study finds that the variables influencing the interbank call money market transactions are the interest rate of interbank call money market and the check money. Both variables have positive effect on the interbank money market transactions in Indonesia.Keywords: Loans, Interest Rate, Check Money, Monetary Crisis, Interbank Money Market TransactionsJEL Classification Numbers: G21, G28AbstrakPenelitian ini menganalisis pengaruh pinjaman Bank Indonesia terhadap tingkat bunga pasar uang antar bank, jumlah uang giral, dan krisis ekonomi Indonesia tahun 1997 terhadap transaksi pasar uang antar bank di Indonesia menggunakan metode regresi linier berganda. Penelitian ini menemukan bahwa variabel yang berpengaruh terhadap transaksi pasar uang antar bank tersebut adalah tingkat bunga pasar uang antar bank dan jumlah uang giral. Dua variabel tersebut berpengaruh positif terhadap transaksi pasar uang antar bank di Indonesia.Keywords: Pinjaman, Tingkat Bunga, Uang Giral, Krisis Moneter, Transaksi Pasar Uang antar BankJEL Classification Numbers: G21, G28

  16. Voice over IP phone calls from your smartphone

    CERN Multimedia

    2014-01-01

    All CERN users do have a Lync account (see here) and can use Instant Messaging, presence and other features. In addition, if your number is activated on Lync IP Phone(1) system then you can make standard phone calls from your computer (Windows/Mac).   Recently, we upgraded the infrastructure to Lync 2013. One of the major features is the possibility to make Voice over IP phone calls from a smartphone using your CERN standard phone number (not mobile!). Install Lync 2013 on iPhone/iPad, Android or Windows Phone, connect to WiFi network and make phone calls as if you were in your office. There will be no roaming charges because you will be using WiFi to connect to CERN phone system(2). Register here to the presentation on Tuesday 29 April at 11 a.m. in the Technical Training Center and see the most exciting features of Lync 2013.   Looking forward to seeing you! The Lync team (1) How to register on Lync IP Phone system: http://information-technology.web.cern.ch/book/lync-ip-phone-serv...

  17. Anthropogenic noise alters bat activity levels and echolocation calls

    Directory of Open Access Journals (Sweden)

    Jessie P. Bunkley

    2015-01-01

    Full Text Available Negative impacts from anthropogenic noise are well documented for many wildlife taxa. Investigations of the effects of noise on bats however, have not been conducted outside of the laboratory. Bats that hunt arthropods rely on auditory information to forage. Part of this acoustic information can fall within the spectrum of anthropogenic noise, which can potentially interfere with signal reception and processing. Compressor stations associated with natural gas extraction produce broadband noise 24 hours a day, 365 days a year. With over half a million producing gas wells in the U.S. this infrastructure is a major source of noise pollution across the landscape. We conducted a ‘natural experiment’ in the second largest gas extraction field in the U.S. to investigate the potential effects of gas compressor station noise on the activity levels of the local bat assemblage. We used acoustic monitoring to compare the activity level (number of minutes in a night with a bat call of the bat assemblage at sites with compressor stations to sites lacking this infrastructure. We found that activity levels for the Brazilian free-tailed bat (Tadarida brasiliensis were 40% lower at loud compressor sites compared to quieter well pads, whereas the activity levels of four other species (Myotis californicus, M. cillolabrum, M. lucifugus, Parastrellus hesperus were not affected by noise. Furthermore, our results reveal that the assemblage of bat species emitting low frequency (35 kHz echolocation did not exhibit altered activity levels in noise. Lower activity levels of Brazilian free-tailed bats at loud sites indicate a potential reduction in habitat for this species. Additionally, a comparison of echolocation search calls produced by free-tailed bats at sites with and without compressor stations reveal that this species modifies its echolocation search calls in noise—producing longer calls with a narrower bandwidth. Call alterations might affect prey

  18. Composting plant of sewage sludges in Calles, Valencia (Spain); Planta de compostaje de fangos en la localidad de Calles (Valencia)

    Energy Technology Data Exchange (ETDEWEB)

    Morenilla Martinez, J. J.; Bernacer Bonora, I.; Jimenez Sanchez, J.; Zorrilla Soriano, F.; Manuelcandela, V.

    2000-07-01

    This article explains the operation of the composting plant of muds of residual waters in the location of Calles, in Valencia. Through the composting, the sludge is transformed in wet material. This process is developed by aerobic thermopile fermentation of the organic fraction of the muds. The composting is a biological process aerobic and thermopile by decomposition of organic waste in solid phase and in controlled conditions. (Author)

  19. Primitive entertainment prank calls in the work of counseling psychologist on Children helpline

    Directory of Open Access Journals (Sweden)

    I.A. Geronimus

    2014-08-01

    Full Text Available We explore the challenges faced by counseling psychologist when working at the Children's Helpline in cases of prank calls. The category of prank calls include such calls, when the caller asks the psychologist to discuss the imaginary situation, or do not formulate a query at all. On the basis of empirical data, we revealed the main varieties of such calls: call jokes, fantasy calls, intrusive calls, insulting calls, prank calls, calls of a sexual nature, dating calls. We explore the possible motivations of children and adolescents, entertaining by phone calls: experimentation with new social roles, expression of negative emotions, cognitive motivation, etc. We show the principles and strategies of counselors of Children's helpline working with this type of calls: they are based on cultural-historical psychology ideas and V. Satir communicative styles model.

  20. THE ENTREPRENEURIAL WAY: CALLING, CHANCE OR OPPORTUNITY. AN ESSAY

    Directory of Open Access Journals (Sweden)

    Daniel Badulescu

    2014-07-01

    Full Text Available Entrepreneurship plays an important role in shaping economies and producing economic added value. Among other determinants of the economic activity, the influence of religion on economic activity was a major concern in socio-economic research. However, how the religion affects the decision to enter into entrepreneurship has been approached in fewer studies and researches. The decision to enter into entrepreneurship is a complex process, determined by many factors, from different sources, exerting various influences. Thus, it was argued that certain religions can foster business initiative, while others inhibit it. On the other hand, individuals and communities, separated by native environment, prove outstanding entrepreneurial capabilities and determination. Although the “protestant” attributes of entrepreneurship have long ceased to make the rule in researching the religious perspective on the entrepreneurial phenomenon, the temptation to explain some successful business by vocation, gift or even by ”call” still exists in contemporary research. A significant part of entrepreneurs, self-declared as religious, are deeply convinced of the divine grace bestowed on them and their businesses, while other entrepreneurs avoid to associate ”calling” with business success. There are certain reasons explaining this reluctance, e.g. caution, separation between religious practice and business, fear that entrepreneurial failure could mean lack of divine grace etc. The theological studies accept and promote the concept of calling in trade and business, but they place it in a pastoral context, i.e. the true calling should be realized in the act of faith and religiously guided. Nevertheless, examples of entrepreneurial activity, both honest and successful, can also occur outside the revelation of calling.

  1. The composition of intern work while on call.

    Science.gov (United States)

    Fletcher, Kathlyn E; Visotcky, Alexis M; Slagle, Jason M; Tarima, Sergey; Weinger, Matthew B; Schapira, Marilyn M

    2012-11-01

    The work of house staff is being increasingly scrutinized as duty hours continue to be restricted. To describe the distribution of work performed by internal medicine interns while on call. Prospective time motion study on general internal medicine wards at a VA hospital affiliated with a tertiary care medical center and internal medicine residency program. Internal medicine interns. Trained observers followed interns during a "call" day. The observers continuously recorded the tasks performed by interns, using customized task analysis software. We measured the amount of time spent on each task. We calculated means and standard deviations for the amount of time spent on six categories of tasks: clinical computer work (e.g., writing orders and notes), non-patient communication, direct patient care (work done at the bedside), downtime, transit and teaching/learning. We also calculated means and standard deviations for time spent on specific tasks within each category. We compared the amount of time spent on the top three categories using analysis of variance. The largest proportion of intern time was spent in clinical computer work (40 %). Thirty percent of time was spent on non-patient communication. Only 12 % of intern time was spent at the bedside. Downtime activities, transit and teaching/learning accounted for 11 %, 5 % and 2 % of intern time, respectively. Our results suggest that during on call periods, relatively small amounts of time are spent on direct patient care and teaching/learning activities. As intern duty hours continue to decrease, attention should be directed towards preserving time with patients and increasing time in education.

  2. Called to respond: The potential of unveiling hiddens

    Directory of Open Access Journals (Sweden)

    Alison L Black

    2014-12-01

    Full Text Available Interested in exploring how personal stories and aesthetic modes of representing experiences can nudge open academic and educational spaces, this article/collection of particles seeks to document our encounters of being affected and called to respond to things the other has written and represented. As a way of engaging with questions about what research and research data might be and become, our attention has been drawn to stories and images from our lives that we have not shaken off – and to how, as we have opened these to the other, making once private moments public, our hiddens have morphed tenderly into a shared knowing and being. As we have acted on the call we have felt to respond we have found ourselves entering spaces of collaboration, communion, contemplation, and conversation – spaces illuminated by what we have not been able to – and cannot – set aside. Using visual and poetic materials we explore heartfelt and heartbroken aspects of our educational worlds and lives, to be present with each other and our (reemerging personal and professional meanings. We see the shared body (of work, of writing, of image that develops from the taking of brave steps and the risky slipping off of academic masks and language, as a manifestation of the trusted and nurturing spaces that can be generated through collaborative opportunities to gather together. These steps towards unveiling hiddens are producing in us and of us a friendship, fluency, and fluidity as we write new ways of becoming. In turn, we hope the uncovering and revealing of our dialogue in the public gathering of this journal might supports readers’ telling of their own life stories through what calls them to respond.

  3. Need for recovery among male technical distal on-call workers

    NARCIS (Netherlands)

    van de Ven, Hardy A.; Bultmann, Ute; de Looze, Michiel P.; Koolhaas, Wendy; Kantermann, Thomas; Brouwer, Sandra; van der Klink, Jac J. L.

    2015-01-01

    The objectives of this study were to (1) examine whether need for recovery differs between workers (i) not on-call, (ii) on-call but not called and (iii) on-call and called, and (2) investigate the associations between age, health, work and social characteristics with need for recovery for the three

  4. Calling and Life Satisfaction: It's Not about Having It, It's about Living It

    Science.gov (United States)

    Duffy, Ryan D.; Allan, Blake A.; Autin, Kelsey L.; Bott, Elizabeth M.

    2013-01-01

    The present study examined the relation of career calling to life satisfaction among a diverse sample of 553 working adults, with a specific focus on the distinction between perceiving a calling (sensing a calling to a career) and living a calling (actualizing one's calling in one's current career). As hypothesized, the relation of perceiving a…

  5. Calling and Life Satisfaction: It's Not about Having It, It's about Living It

    Science.gov (United States)

    Duffy, Ryan D.; Allan, Blake A.; Autin, Kelsey L.; Bott, Elizabeth M.

    2013-01-01

    The present study examined the relation of career calling to life satisfaction among a diverse sample of 553 working adults, with a specific focus on the distinction between perceiving a calling (sensing a calling to a career) and living a calling (actualizing one's calling in one's current career). As hypothesized, the relation of perceiving a…

  6. Observations on "Vocabulary Building" in a CALL Environment

    OpenAIRE

    吉田, 由美子

    2003-01-01

    The purpose of this paper is to show some observations on "Vocabulary Building" focused on the students' motivation in a CALL environment. Recently, it is said that both teachers and students have become computer literate and use computers to do meaningful and productive work in language teaching or language learning. It is true that e-mails and browsing the Website have become quite usual in our daily lives these days ; however, it seems that they haven't made the most use of computers in cl...

  7. A Call for Critical Perspectives in Qualitative Research

    Directory of Open Access Journals (Sweden)

    Rainer Winter

    2010-11-01

    Full Text Available The formation of critical poststructuralism in the United States has fundamentally transformed qualitative research. The crisis of representation first discussed in anthropology has had the effect of rethinking the foundations of qualitative research by putting ethical questions on the agenda and stimulating a search for new forms of validity. Against this backdrop, this study will analyze different methods and research strategies in critical qualitative inquiry, such as interpretive interactionism, autoethnography, and performance ethnography. The call to action inherent in these strategies and further contributions to cultural and social change will be discussed. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs110171

  8. China Calls for Overhaul of International Monetary System

    Institute of Scientific and Technical Information of China (English)

    Li Zhen

    2009-01-01

    @@ After the outbreak of the global financial crisis,there has been an outcry from developing countries,especially the so-called BRIC(Brazil,Russia,India and China),that a"diversified"international monetary system should be put in place.At their first formal summit meeting held on June,the leaders of Brazil,Russia,India and China,whose governments are some of the world's largest dollar helders,jointly declared the need for a more diversified international monetary system,sparking a drop in the greenback on world markets.

  9. [Once again the so-called Testament of Hippocrates].

    Science.gov (United States)

    Sideras, Alexander

    2006-01-01

    This study is dealing with the so-called Testament of Hippocrates, a short ethic about the doctor's background, knowledge, character, appearance and behaviour towards the patient. Along with the three well-known Greek manuscripts the author is looking at two other by now unknown codices, so that the two Greek versions are accompanied by a third and larger version. After a careful consideration of the relations between the different versions he is discussing all readings of the codices explaining the terms within the background of the history of medicine. At the end he is presenting a reconstruction of the Greek original together with a German translation.

  10. Nursing as a learning discipline: a call to action.

    Science.gov (United States)

    Androwich, Ida M

    2013-01-01

    The advent of the electronic health record and numerous mobile, wireless tools with the capacity to capture, store, and transmit prodigious amounts of data, both using and creating evidence-based content and analytics for clinical decision support, requires that all care providers rethink approaches to care delivery. The nursing profession is called to acutely appreciate and support the vision of documentation systems that provide optimal decision support at the point of care, represent nursing knowledge, and are also capable of capturing care delivery outcomes that can be used to inform future care and improve the health of the public.

  11. TCP-Call Admission Control Interaction in Multiplatform Space Architectures

    Directory of Open Access Journals (Sweden)

    Georgios Theodoridis

    2007-06-01

    Full Text Available The implementation of efficient call admission control (CAC algorithms is useful to prevent congestion and guarantee target quality of service (QoS. When TCP protocol is adopted, some inefficiencies can arise due to the peculiar evolution of the congestion window. The development of cross-layer techniques can greatly help to improve efficiency and flexibility for wireless networks. In this frame, the present paper addresses the introduction of TCP feedback into the CAC procedures in different nonterrestrial wireless architectures. CAC performance improvement is shown for different space-based architectures, including both satellites and high altitude platform (HAP systems.

  12. Thoughts on the so-called radius-capitellum axis

    Energy Technology Data Exchange (ETDEWEB)

    Schild, H.; Mueller, H.A.; Wagner, H.; Baetz, W.

    1982-02-01

    We have studied 438 patients radiologically in order to observe the so-called 'radius-capitellum axis'. In about a quarter of people with normal elbows the axis passes lateral to the middle portion of the capitellum, so that even when there is marked deviation, there is no certainty that the humero-radial joint is abnormal. Deviation of the axis can be caused by changes in the shape of the capitellum or of the radius, or by distension of the capsule of the elbow joint, or by various changes in muscular pull.

  13. Animal Images in The Call of the Wild

    Institute of Scientific and Technical Information of China (English)

    赵秀丽; 陶阳

    2014-01-01

    There is a long history of animal images in literature. What we have known about animal literature is confined into the Greek mythology, Aesop’s Fables and some fairy tales. But in the 20th century, the development of animal literary which focuses on the real animals is great. The Call of the Wild by Jack London is a representative work of describing real animals. This paper will discuss two different images of Buck by means of two archetypes based on Archetype Theory of Carl G. Jung, shadow and hero, in order to study how Buck changed from a dog to a wolf.

  14. [When should the nephrologist be called in the emergency room?].

    Science.gov (United States)

    Favre, Nathalie; Burnier, Michel; Kissling, Sébastien

    2016-02-24

    Nephrology emergencies are not the most frequent in the emergency room but they often generate some diagnostic and therapeutic problems. Most common renal emergencies are due most often to acute renal failure whatever the cause, electrolytes disturbances, hypertensive crisis and less frequently intoxications or acute decompensation of chronic kidney diseases. The goal of this paper is to review some of these clinical situations both in the diagnostic and therapeutic perspective but essentially to discuss when the nephrologist should be called in the emergency room so that the coordination of care is optimal for the patients.

  15. COCA Call: New Frontiers in Hand Hygiene Practices

    Centers for Disease Control (CDC) Podcasts

    2010-05-11

    This podcast is an overview of the Clinician Outreach and Communication Activity (COCA) Call, "New Frontiers in Implementation and Measurement of Hand Hygiene Practices." CDC's Katherine Ellingson discusses recent challenges in applying hand hygiene recommendations and identifies global and national campaign activities and resources that support hand hygiene in the reduction of healthcare-associated infections (HAIs).  Created: 5/11/2010 by National Center for Preparedness, Detection, and Control of Infectious Diseases (NCPDCID/DHQP) and Emergency Communication System (ECS)/Joint Information Center (JIC); Office of Public Health Preparedness and Response (OPHPR).   Date Released: 5/12/2010.

  16. Assessing and Managing Acute Pain: A Call to Action.

    Science.gov (United States)

    Jungquist, Carla R; Vallerand, April Hazard; Sicoutris, Corinna; Kwon, Kyung N; Polomano, Rosemary C

    2017-03-01

    : Acute pain, which is usually sudden in onset and time limited, serves a biological protective function, warning the body of impending danger. However, while acute pain often resolves over time with normal healing, unrelieved acute pain can disrupt activities of daily living and transition to chronic pain. This article describes the effects of unrelieved acute pain on patients and clinical outcomes. The authors call on nurses to assess and manage acute pain in accordance with evidence-based guidelines, expert consensus reports, and position statements from professional nursing organizations in order to minimize the likelihood of its becoming chronic.

  17. CONSIDERATIONS CONCERNING THE MECHANISM CALLED THE CHEBYSHEV’SăPARADOX

    Directory of Open Access Journals (Sweden)

    Iulian POPESCU

    2016-05-01

    Full Text Available We study the kinetics of the mechanism called the Chebyshev’s paradox. We show the mechanism consecutive positions, than detailed according to the trigonometrical dials. We notice complex movements, the conducted final part has an irregular movement, with returns. The whole is explained by the appearing of 6 positions where the last conducted part and the anterior crank superpose or prolong each other, the dead point positions where the movements may change the direction. We give the diagrams explaining this ingenuous system.

  18. A Verification System for Distributed Objects with Asynchronous Method Calls

    Science.gov (United States)

    Ahrendt, Wolfgang; Dylla, Maximilian

    We present a verification system for Creol, an object-oriented modeling language for concurrent distributed applications. The system is an instance of KeY, a framework for object-oriented software verification, which has so far been applied foremost to sequential Java. Building on KeY characteristic concepts, like dynamic logic, sequent calculus, explicit substitutions, and the taclet rule language, the system presented in this paper addresses functional correctness of Creol models featuring local cooperative thread parallelism and global communication via asynchronous method calls. The calculus heavily operates on communication histories which describe the interfaces of Creol units. Two example scenarios demonstrate the usage of the system.

  19. Proyecto de interiorismo : edificio calle de La Paz 32, Valencia

    OpenAIRE

    OLIVARES SÁNCHEZ, MARÍA DEL CARMEN

    2013-01-01

    Este TFG trata sobre el edificio situado en la Calle de la Paz, n. 32, construido en 1906 y situado en el casco histórico de Valencia, en la Ciutat Vella. El inmueble posee un total de seis plantas. En la actualidad la planta baja está ocupada por una agencia de viajes "Viajes Paz". El resto del edificio está siendo utilizado por una única empresa de rehabilitación de inmuebles. En la planta 2ª se encuentra la secretaría, con diversas habitaciones de uso administrativo. La tercera y cuarta es...

  20. Call 4 Concern: patient and relative activated critical care outreach.

    Science.gov (United States)

    Odell, Mandy; Gerber, Karin; Gager, Melanie

    Patients can experience unexpected deterioration in their physiological condition that can lead to critical illness, cardiac arrest, admission to the intensive care unit and death. While ward staff can identify deterioration through monitoring physiological signs, these signs can be missed, interpreted incorrectly or mismanaged. Rapid response systems using early warning scores can fail if staff do not follow protocols or do not notice or manage deterioration adequately. Nurses often notice deterioration intuitively because of their knowledge of individual patients. Patients and their relatives have the greatest knowledge of patients, and can often pick up subtle signs physiological deterioration before this is identified by staff or monitoring systems. However, this ability has been largely overlooked. Call 4 Concern (C4C) is a scheme where patients and relatives can call critical care teams directly if they are concerned about a patient's condition- it is believed to be the first of its kind in the UK. A C4C feasibility project ran for six months, covering patients being transferred from the intensive care unit to general wards. C4C has the potential to prevent clinical deterioration and is valued by patients and relatives. Concerns of ward staff could be managed through project management. As it is relatively new, this field offers further opportunities for research.

  1. Population characteristics may reduce the levels of individual call identity.

    Directory of Open Access Journals (Sweden)

    María del Mar Delgado

    Full Text Available Individual variability influences the demographic and evolutionary dynamics of spatially structured populations, and conversely ecological and evolutionary dynamics provide the context under which variations at the individual level occur. Therefore, it is essential to identify and characterize the importance of the different factors that may promote or hinder individual variability. Animal signaling is a prime example of a type of behavior that is largely dependent on both the features of individuals and the characteristics of the population to which they belong. After 10 years studying the dynamics of a population of a long-lived species, the eagle owl (Bubo bubo, we investigated the emergence and maintenance of traits that reveal individual identity by focusing on vocal features. We found that individuals inhabiting a high density population characterized by a relative lack of heterogeneity (in terms of prey availability and breeding success among breeding sites might be selected for reducing the levels of identity. Two non-mutually exclusive hypotheses may explain the structural call patterns we detected: (1 similarity in calls may be principally a consequence of the particular characteristics of the population; and (2 high density may encourage individuals to mimic each other's vocalizations in a cascade effect, leading to a widespread and unique communication network.

  2. The advertisement calls of Quasipaa shini (Ahl, 1930) (Anura: Dicroglossidae).

    Science.gov (United States)

    Kong, Shen Shen; Zheng, Rong Quan; Zhang, Qi Peng

    2016-12-04

    The genus Quasipaa (Family Dicroglossidae) is currently composed of 11 species distributed in China and Southeast Asia: Quasipaa acanthophora (Dubois & Ohler 2009), Q. boulengeri (Günther 1889), Q. courtoisi (Angel 1922), Q. delacouri (Angel 1928), Q. exilispinosa (Liu & Hu, 1975), Q. fasciculispina (Inger 1970), Q. jiulongensis (Huang & Liu, 1985), Q. shini (Ahl 1930), Q. spinosa (David 1875), Q. verrucospinosa (Bourret 1937), Q. yei (Chen, Qu & Jiang 2002) (Frost 2016). These species are morphologically similar, and their taxonomy is subject to controversy (Che et al. 2009). Analyses of nuclear and mitochondrial genes suggest the genus likely encompass additional cryptic species (Ye et al. 2013). Bioacoustics has contributed to studies on the taxonomy of the genus (Ye et al. 2013; Shen et al. 2015), however, to date, only the advertisement calls of Q. spinosa are known (Yu & Zheng 2009; Chen et al. 2012; Shen et al. 2015). Here, we describe the advertisement calls of Q. shini, which inhabits streams in the southern part of central China(Guizhou, Hunan, Guangxi and Jiangxi) and is characterized by the presence of keratinized skin spines on the lateral surfaces of the body.

  3. Investigation of CT picture in so-called loose shoulder

    Energy Technology Data Exchange (ETDEWEB)

    Kuroda, Shigehito; Sakamaki, Hiroshi; Matsuoka, Akira; Moriishi, Takeji; Takada, Keiichi

    1985-03-01

    CT picture of the shoulder joint was analyzed in 124 shoulders (114 patients). A line perpendicular to a given line between the precornu of acetabular tegmen (A) and the postcornu of acetabular tegmen (B) was drawn and the intersection where the line and the caput humeri meet (C) was obtained. The angle of CAB was defined as the backward angular aperture of the acetabular tegmen. The angular aperture was 26.2 +- 1.9 in 16 so-called loose shoulders, 17.3 +- 1.0 in 28 loose shoulders restricted to the inward rotation, and 12.2 +- 0.4 in 80 normal shoulders, showing a distinct correlation between the angular aperture and the degree of loose shoulder. An increased backward angular aperture of the acetabular tegmen was considered greatly attributable to the forward glenohumeral movement resulting from malformation of the acetabular tegmen and flaccidity of the joint. Glenoid osteotomy was thus performed in 9 patients, 6 of whom underwent CT scanning before and after osteotomy. Coronal and transverse CT images of the shoulder joint disclosed a noticeable improvement of the glenohumeral alignment. The angular aperture shown on CT seems to be of major importance not only in the diagnosis of so-called loose shoulder but also in surgical choice. (Namekawa, K.).

  4. An Adaptive Data Collection Procedure for Call Prioritization

    Directory of Open Access Journals (Sweden)

    Beaumont Jean-Francois

    2014-12-01

    Full Text Available We propose an adaptive data collection procedure for call prioritization in the context of computer-assisted telephone interview surveys. Our procedure is adaptive in the sense that the effort assigned to a sample unit may vary from one unit to another and may also vary during data collection. The goal of an adaptive procedure is usually to increase quality for a given cost or, alternatively, to reduce cost for a given quality. The quality criterion often considered in the literature is the nonresponse bias of an estimator that is not adjusted for nonresponse. Although the reduction of the nonresponse bias is a desirable goal, we argue that it is not a useful criterion to use at the data collection stage of a survey because the bias that can be removed at this stage through an adaptive collection procedure can also be removed at the estimation stage through appropriate nonresponse weight adjustments. Instead, we develop a procedure of call prioritization that, given the selected sample, attempts to minimize the conditional variance of a nonresponse-adjusted estimator subject to an overall budget constraint. We evaluate the performance of our procedure in a simulation study.

  5. ASTHENOPIA PADA PEKERJA WANITA DI CALL CENTRE-X

    Directory of Open Access Journals (Sweden)

    Frans X Suharyanto

    2012-07-01

    Full Text Available Abstract. Nowdays, computers have been used widely in every kind of occupation. One of the health problems of the computer using is eyestrain or asthenopia. Some experts have tried to correlate the exposure of Video Display Terminal (VDT with the occurrence of asthenopia but until present there is no database about the prevelance of computer operators found they more easily become asthenopic. The complain of asthenopia itself is subjective and varies in every individual and therefore one measureable objective value is needed to determine the occurrence of the asthenopia case itself.The design of the study was using "pre and post test", it involved 72 subjects in "X"-call centre and using photostress test to measure objectively the occurrence ofasthenopia by measuring the increasing of Macular Recovery Time (MKT before and after working.The average shift of MKT was about 2.98 ± 3.57 second within 68.1 % of thesubjects, which were evaluated after 4-hour working time with their VDT. The distance between the eyes to VDT and the satisfaction with the working shift arrangement had significant correlation with the occurrence of asthenopia. There was significant correlation between subjective complaints such as pain within the area around the eyes,headache and dry eyes due to increasing of the MRTKeywords : Asthenopia, Macular Recovery Time (MRT, Call Centre, Video DisplayTerminal (VDT.

  6. Group calls for space policies to transcend politics

    Science.gov (United States)

    Showstack, Randy

    2012-06-01

    At a 22 May briefing, the American Institute of Aeronautics and Astronautics (AIAA) called on Congress to “establish space exploration policy goals which transcend partisan political differences.” AIAA president and former NASA administrator Michael Griffin said the “goal of establishing human capability to b e a space-faring species is not a short-term goal,” nor is it a goal that belongs to only one political party. “We will not reach long-term goals without a stable, coherent, sensible plan that transcends elections and leaders,” said Griffin, who has provided advice to Republican U.S. presidential candidate Mitt Romney. Griffin pointed to NASA's 2008 authorization as providing the kind of vision needed for NASA. The act called for human return to the Moon and preparation for the capability for permanent bases on the Moon, among other things, he said. “That's the kind of thing that we need. All of the goals espoused by the 2008 act were long-term, generational, strategic in scope,” Griffin said, adding that the act, which had bipartisan support, demonstrated “the kind of societal support, rather than political support, that I believe our space program deserves.”

  7. [The controversy about so-called "active euthanasia"].

    Science.gov (United States)

    Karbowski, K

    1996-09-17

    In the past few years, the discussion about the right to kill severely ill patients for humanitarian reasons--once called euthanasia and after World War II in the German speaking countries as euphemism called 'aktive Sterbehilfe' (active help to die)--has become more intensive. Actually, only the Netherlands do officially practice euthanasia. But the variant that consists in putting a pharmaceutical at the patient's disposal in order to kill himself is very well known in other countries, e.g. in Switzerland, where the pressure on the legislator to also legalize euthanasia grows. Taking into account medico-ethical reasons, historical experiences, alarming euthanasia ideas in bioethics and social philosophy, the danger due to the impossibility to control the development in this field, and the insidious extension of euthanasia indications, the author rejects firmly the idea of legalized euthanasia. These problems are related to those that occur in fixing the moment of death of potential organ donors. A lack of organs in transplantation medicine should not lead physicians to explant organs from donors in a dying condition. For physicians who take care of potential organ donors and who have to look after their interests towards transplantation teams, it is often too difficult to cope with this task. Local ethical commissions should support them.

  8. 76 FR 2712 - Sara Lee Corporation, Master Data, Cash Applications, Deductions, Collections, Call Center...

    Science.gov (United States)

    2011-01-14

    ..., Collections, Call Center, Information Technology, Accounts Payable, General Accounts, Financial Accounts..., Collections, Call Center, Information Technology, Accounts Payable, General Accounts, Financial Accounting..., Deductions, Collections, Call Center, Information Technology, Accounts Payable, General Accounts,......

  9. Study protocol of the YOU CALL - WE CALL TRIAL: impact of a multimodal support intervention after a "mild" stroke

    Directory of Open Access Journals (Sweden)

    Bravo Gina

    2010-01-01

    Full Text Available Abstract Background More than 60% of new strokes each year are "mild" in severity and this proportion is expected to rise in the years to come. Within our current health care system those with "mild" stroke are typically discharged home within days, without further referral to health or rehabilitation services other than advice to see their family physician. Those with mild stroke often have limited access to support from health professionals with stroke-specific knowledge who would typically provide critical information on topics such as secondary stroke prevention, community reintegration, medication counselling and problem solving with regard to specific concerns that arise. Isolation and lack of knowledge may lead to a worsening of health problems including stroke recurrence and unnecessary and costly health care utilization. The purpose of this study is to assess the effectiveness, for individuals who experience a first "mild" stroke, of a sustainable, low cost, multimodal support intervention (comprising information, education and telephone support - "WE CALL" compared to a passive intervention (providing the name and phone number of a resource person available if they feel the need to - "YOU CALL", on two primary outcomes: unplanned-use of health services for negative events and quality of life. Method/Design We will recruit 384 adults who meet inclusion criteria for a first mild stroke across six Canadian sites. Baseline measures will be taken within the first month after stroke onset. Participants will be stratified according to comorbidity level and randomised to one of two groups: YOU CALL or WE CALL. Both interventions will be offered over a six months period. Primary outcomes include unplanned use of heath services for negative event (frequency calendar and quality of life (EQ-5D and Quality of Life Index. Secondary outcomes include participation level (LIFE-H, depression (Beck Depression Inventory II and use of health services for

  10. Critical opportunities for public health law: a call for action.

    Science.gov (United States)

    Mello, Michelle M; Wood, Jennifer; Burris, Scott; Wagenaar, Alexander C; Ibrahim, Jennifer K; Swanson, Jeffrey W

    2013-11-01

    Although legal interventions are responsible for many sentinel public health achievements, law is underutilized as a tool for advancing population health. Our purpose was to identify critical opportunities for public health lawmaking. We articulated key criteria and illustrated their use with 5 examples. These opportunities involve significant health problems that are potentially amenable to change through law and for which an effective legal intervention is available: optimizing graduated driver licensing laws, increasing tax rates on alcoholic beverages, regulating sodium in foods, enacting laws to facilitate reversal of opioid overdoses, and improving mental health interventions in the college setting. We call for a national conversation about critical opportunities for public health law to advance evidence-based policymaking.

  11. La guerra silenciosa en el mundo de los Call Centers

    Directory of Open Access Journals (Sweden)

    Pedro Lisdero

    2012-03-01

    Full Text Available El objetivo de este artículo es indagar la operatoria del miedo como mecanismo social a partir del análisis del conflicto emergente en el sector de Call Centers de la ciudad de Córdoba, Argentina. Explorando los relatos de trabajadores que protestan puede observarse que el miedo —en tanto construcción social— opera organizando percepciones, configurando sensaciones complementarias, y performando la capacidad de acción autónoma de los sujetos. A partir de aquí es posible obtener algunas pistas que den cuenta de la expropiación de las energías corporales como contenido específico de la explotación del trabajo en la situación actual del capitalismo.

  12. Call of the Wild and the Ethics of Narrative Strategies

    Directory of Open Access Journals (Sweden)

    Roman Bartosch

    2010-01-01

    Full Text Available How can the analysis of narrative structures contribute to the understanding of what makes a text´s "environmentality" (see Buell 2005:25? By reading Call of the Wild from a narratological perspective and against the historicist foil of its discursive context, this paper seeks to illuminate how strategies of narration lend to an eco-centred reading - even despite the text´s apparent ethical orientation. The discursive circuit thus established enables a textual negotiation of diverging ethical convictions and aspects of compassion and giving voice to an animal. Eventually, reading and interpreting texts can thus be described as an "applied ethics" (Iovino 2010: 41 the features of which this essay seeks to describe as the "ethics of narrative strategies".

  13. A two-phase system call arguments attribute analyzing method

    Institute of Scientific and Technical Information of China (English)

    LI Hong-jiao; LI Jian-hua

    2008-01-01

    To detect more attacks aiming at key security data in program behavior-based anomaly detection, the data flow properties were formulated as unary and binary relations on system call arguments. A new method named two-phrase analysis (2PA) is designed to analyze the efficient relation dependency, and its description as well as advantages are discussed. During the phase of static analysis, a dependency graph was constructed according to the program's data dependency graph, which was used in the phase of dynamic learning to learn specified binary relations. The constructed dependency graph only stores the information of related arguments and events, thus improves the efficiency of the learning algorithm and reduces the size of learned relation de-pendencies. Performance evaluations show that the new method is more efficient than existing methods.

  14. Entrepreneurship research and practice: a call to action for psychology.

    Science.gov (United States)

    Hisrich, Robert; Langan-Fox, Janice; Grant, Sharon

    2007-09-01

    Entrepreneurship is a major source of employment, economic growth, and innovation, promoting product and service quality, competition, and economic flexibility. It is also a mechanism by which many people enter the society's economic and social mainstream, aiding culture formation, population integration, and social mobility. This article aims to illuminate research opportunities for psychologists by exposing gaps in the entrepreneurship literature and describing how these gaps can be filled. A "call to action" is issued to psychologists to develop theory and undertake empirical research focusing on five key topic areas: the personality characteristics of entrepreneurs, the psychopathology of entrepreneurs, entrepreneurial cognition, entrepreneurship education, and international entrepreneurship. Methodological issues are discussed and recommendations provided. It is shown that psychologists can help identify the factors that influence new venture creation and success and inform the construction of public policy to facilitate entrepreneurship.

  15. Mythic Frodo and his Predestinate Call to Adventure

    Directory of Open Access Journals (Sweden)

    Farid Mohammadi

    2013-09-01

    Full Text Available One of the most interesting aspects of J.R.R. Tolkien’s stories, especially The Lord of the Rings is the presentation of the multidimensional characters; therefore, the complexity of interpretations concerning their actions, motives, and aims will be manifested from the beginning of the story, until the end. Following the study of a mythic hero’s adventure, initiation, and psychological aspects, the present paper focuses on the investigation of Predestinate Call to Adventure. The researcher has benefited the theories of Joseph Campbell, presented in his renowned book The Hero with a Thousand Faces and the supplementary comments of Christopher Vogler. In fact, the researcher’s motivation for the work initiated in this regard is to improve the very few previous attempts studied by others, concerning Frodo Baggins’ role as the main and the most tragic hero of The Lord of the Rings. Keywords: Mythology, Tragic hero, Mythic hero, Tolkien’s legendarium, Monomyth, Initiation

  16. Emotional distance to so-called difficult patients

    DEFF Research Database (Denmark)

    Michaelsen, Jette Joost

    2012-01-01

    patient, the avoidance strategy did not necessarily represent the terminal stage, since a nurse could revert to the compromise strategy. Some of the nurses experienced painful emotions regarding these interactions. Conclusions: The avoidance strategy (emotional distance) resulted in important social......Scand J Caring Sci; 2011 Emotional distance to so-called difficult patients Purpose: To explore nurses' relationships with patients they regard as being difficult. How do nurses feel about such patients and relate to them, and what are the consequences for nurse and patient? Design and methods....... Patients' case records were studied and four meetings with the staff were arranged to discuss the findings. Data collection lasted 18 months in all. Findings: Three strategies were identified: persuasion, avoidance (emotional distance), and compromise. Interestingly, in the relationship with a particular...

  17. Recetas para subir de peso (Imitando a Sophia Calle

    Directory of Open Access Journals (Sweden)

    Óscar Hernando Nossa García

    2011-05-01

    Full Text Available In 1995, when he was flying over London suburbs, Hans-Ulrich Obrist thought to make a new interpretation of Do it —a project that previously had been televised and had involved museums—, making a domestic version. Now, somehow, the result of the instructions disclosed would become intimate, it would be exhibited quietly inside the home. Do It seeks for proposals that will be seen as permeated and transformed by the culture to where they arrive. Geography as creative element. After culminating her art studies, a woman decides never to abandon the world of artistic production. Sophie Calle and Do it help her to tolerate her life. Do it yourself.

  18. Taking the Danish Speech Trainer from CALL to ICALL

    DEFF Research Database (Denmark)

    Juel Henrichsen, Peter

    2015-01-01

    Talebob (Speech Bob) is a newly developed interactive CALL-tool for training Danish speech with special regard to the pronunciation of highly idiomatic phrases. Talebob is currently being tested in primary schools in Nuuk, Hafnarfjörður and Tórshavn (where Danish is taught as a L2). The purpose...... of the current paper is twofold. We first introduce Talebob in its publicly available version, commenting on its linguistic, technical, and didactic principles. Secondly, we present our current plans and goals for the next version of Talebob focusing on linguistic and educational perspectives. Taking Talebob II...... as a point of departure, we wish to invite a discussion of ICALL as a means of modernizing the L2 educational programmes in the Nordic area....

  19. Designing of an emergency call system for traffic accidents

    Directory of Open Access Journals (Sweden)

    Ziya Ekşi

    2013-06-01

    Full Text Available In our country, many people have been seriously injured or died in traffic accidents. Fatal accidents often occur because of not complying with traffic rules or carelessness. Except these driver mistakes, heavy injuries can result in deaths because of emergency aid teams failing to arrive to accident scene in time. In this study, an accident emergency call system is designed to help injured people's treatment as soon as possible by notifying emercengy team automatically in accidents. The designed system sends messages, including information such as vehicle location, exploding airbag number, vehicle model, age and registration plate, to emergency aid team using GPS module at the moment of crashed vehicle's airbag explosion.

  20. Do modern times call for an industrial policy

    Energy Technology Data Exchange (ETDEWEB)

    Karlyn Keen and Everett Ladd, editors of Public Opinion, talk with Herbert Stein and Lester Thurow about the state of the economy, the state of the economics profession, and industrial policy. Thurow advocates a policy calling for industry to develop a program and government to reinforce it through things such as exemption from antitrust laws. His ingredients of an industrial policy are a civilian industrial arm, legalization of private investment banking, and industrial triage. Stein is concerned that a new policy will work no better than present back door policies unless political power reduces the ability of government to protect inefficient, ineffective, and too-costly industries. The forum concludes with a tentative forecast for economic recovery.

  1. Scaling Roll Call Votes with wnominate in R

    Directory of Open Access Journals (Sweden)

    Keith Poole

    2011-08-01

    Full Text Available This paper presents a software package designed to estimate Poole and Rosenthal W-NOMINATE scores in R. The package uses a logistic regression model to analyze political choice data, usually (though not exclusively from a legislative setting. In contrast to other scaling methods, W-NOMINATE explicitly assumes probabilistic voting based on a spatial utility function, where the parameters of the utility function and the spatial coordinates of the legislators and the votes can all be estimated on the basis of observed voting behavior. Building on software written by Poole in Fortran, the new wnominate package in R facilitates easier data input and manipulation, generates bootstrapped standard errors, and includes a new suite of graphics functions to display the results. We demonstrate the functionality of this package by conducting a natural experiment using roll calls -- an experiment which is greatly simplified by the data manipulation capabilities of the wnominate package in R.

  2. Why all anthropology should be called techno-anthropology

    DEFF Research Database (Denmark)

    Birkbak, Andreas

    2013-01-01

    This chapter argues that if we follow a pragmatist understanding of humans and technologies, there is no reason to keep these phenomena strongly separated. The suggestion that all anthropology should be called techno-anthropology might provoke some readers, but it is first and foremost intended...... as an invitation to think in ambitious terms about what techno- anthropology might be(come). The main contribution of the chapter is to introduce Bruno Latour and John Dewey as techno-anthropologists. While these two thinkers are known for many other engagements, and wrote in different times, they share...... turn to Dewey’s understanding of technology as inquiry, a concept that deliberately ignores the physical/psychological dichotomy. The chapter concludes with a couple of empirical examples of how the pragmatist perspective might guide techno-anthropological analysis....

  3. Los citadinos de la calle, nómadas urbanos

    Directory of Open Access Journals (Sweden)

    Javier Omar Ruiz A.

    1999-04-01

    Full Text Available La ciudad es uno de los puntos de llegada del proceso civilizatorio que ha seguido la humanidad, pero es un punto de llegada que no es uniforme ni homogéneo. La ciudad es tan diversa como las dinámicas de vida de los ciudadanos.La manera como sus habitantes habitan la ciudad hace una de esas diferencias: Unos de manera sedentaria, otros como nómadas. La mayoría moviéndose puertas adentro, desde categorías de lo privado y lo público que no son las mismas para quienes viven explorando permanentemente la cara “callejera” de la ciudad.Junto a la ciudad sedentaria circula una ciudad nómada a otros ritmos, a otras velocidades, con otra lógica; como si un atavismo convocara a la libertad de las calles.

  4. Leadership training for oral health professionals: a call to action.

    Science.gov (United States)

    Taichman, Russell S; Parkinson, Joseph W; Nelson, Bonnie A; Nordquist, Barbara; Ferguson-Young, Daphne C; Thompson, Joseph F

    2012-02-01

    With dramatic changes in the scope and mode of delivering oral health care on the horizon, a strategic approach to addressing the emerging opportunities and challenges is required. Such an approach will demand new and sustained initiatives to develop leaders with the skills, knowledge, and passion to guide oral health care into the future. The purpose of this position paper is to define the need for leadership training programs for dental and dental hygiene students to become future leaders. Whether these oral health professionals become leaders within a solo or group practice or at the local or national level of their profession, they must be given the mindset and tools to lead. This position paper will describe goals for leadership training and give examples of some programs that currently exist in dental education and other professional settings as the background for a call to action for dental education to provide leadership training opportunities for its students.

  5. A jolly good call for Marie Curie Fellows

    CERN Multimedia

    2009-01-01

    A new funding opportunity to train young researchers has just been announced by the European Commission. One of the calls within FP7 Marie Curie Actions requests proposals for Initial Training Network (ITN) projects, with a deadline of 22 December 2009. Project proposals are strongly encouraged at CERN and authors can receive support and guidance from the Marie Curie Steering Group. Winnie Wong: "I wouldn’t have considered a PhD if I hadn’t been a Marie Curie fellow" Dan Savu: "It’s the best of both worlds: training plus working in an international organisation" ITN projects have one key aim: training. Academic and industrial partners work together to form a network to recruit and train Marie Curie Fellows. Fellows are young researchers (typically PhD-level) from any country who combine project-based research with tailor-made training programmes, ...

  6. Life or death decisions: framing the call for help.

    Directory of Open Access Journals (Sweden)

    Eileen Y Chou

    Full Text Available BACKGROUND: Chronic blood shortages in the U.S. would be alleviated by small increases, in percentage terms, of people donating blood. The current research investigated the effects of subtle changes in charity-seeking messages on the likelihood of people responses to a call for help. We predicted that "avoid losses" messages would lead to more helping behavior than "promote gains" messages would. METHOD: Two studies investigated the effects of message framing on helping intentions and behaviors. With the help and collaboration of the Red Cross, Study 1, a field experiment, directly assessed the effectiveness of a call for blood donations that was presented as either death-preventing (losses or life-saving (gains, and as being of either more or less urgent need. With the help and collaboration of a local charity, Study 2, a lab experiment, assessed the effects of the gain-versus-loss framing of a donation-soliciting flyer on individuals' expectations of others' monetary donations as well their own volunteering behavior. Study 2 also assessed the effects of three emotional motivators - feelings of empathy, positive affect, and relational closeness. RESULT: Study 1 indicated that, on a college campus, describing blood donations as a way to "prevent a death" rather than "save a life" boosted the donation rate. Study 2 showed that framing a charity's appeals as helping people to avoid a loss led to larger expected donations, increased intentions to volunteer, and more helping behavior, independent of other emotional motivators. CONCLUSION: This research identifies and demonstrates a reliable and effective method for increasing important helping behaviors by providing charities with concrete ideas that can effectively increase helping behavior generally and potentially death-preventing behavior in particular.

  7. Synchronized calling in a treefrog (Smilisca sila). Short behavioral latencies and implications for neural pathways involved in call perception and production.

    Science.gov (United States)

    Ryan, M J

    1986-01-01

    A neotropical treefrog, Smilisca sila, exhibits an unusual ability to synchronize its calling with that of neighbors such that calls often overlap temporally. Call playback experiments measured the latency to evoked calling in response to one-note and two-note mating calls. Approximately one-half of the responses overlapped the one-note stimulus call, while 20% overlapped the two-note stimulus call. Minimum response latencies were 55 ms and 78 ms in response to the one-note and two-note calls, respectively. These data were used to evaluate the efficacy of proposed neural pathways involved in call recognition and production. Based on neural and behavioral latencies presented in those studies, it is suggested that the proposed pathways for call recognition and production might not accommodate the short behavioral latencies in S. sila. One possible explanation for this discrepancy is that call detection is decoupled from call recognition, the former requiring a shorter neural pathway thus permitting a shorter behavioral latency.

  8. Advertisement call of Scinax camposseabrai (Bokermann, 1968) (Anura: Hylidae), with comments on the call of three species of the Scinax ruber clade.

    Science.gov (United States)

    Novaes, Gabriel; Zina, Juliana

    2016-02-25

    Scinax camposseabrai was allocated into the Scinax ruber clade by Caramaschi & Cardoso (2006) by overall similarities as snout not pointed, breeding in open areas, and an advertisement calls with multipulsed notes. This assumption about the call was based solely on an onomatopoeia provided by Bokermann (1968). Herein we provide a formal description of the advertisement call of S. camposseabrai and compare it with described calls of other S. ruber clade species. Additionally, we provide descriptions of the advertisement calls of three sympatric species of the S. ruber clade: S. eurydice (Bokermann), S. pachycrus (Miranda-Ribeiro) and S. cf. x-signatus.

  9. Passive acoustic monitoring, development of disturbance calls and differentiation of disturbance and advertisement calls in the Argentine croaker Umbrina canosai (Sciaenidae).

    Science.gov (United States)

    Tellechea, J S; Fine, M L; Norbis, W

    2017-01-23

    Disturbance and advertisement calls of the Argentine croaker Umbrina canosai were recorded from coastal Uruguayan waters. Dissections indicate typical sciaenid extrinsic swimbladder muscles present exclusively in males. Disturbance calls were produced when captive U. canosai were startled, chased with a net or grabbed by the tail. Calls were unusual for sciaenids because each pulse consisted of multiple cycles. The number of cycles per pulse and dominant frequency did not change with U. canosai size, but pulse duration and interpulse interval increased. Advertisement calls were recorded from unseen choruses in the field and confirmed with captive individuals in a large tank. Advertisement calls were recorded throughout the known range of the species in Uruguay indicating a continuous belt of spawning populations. Tank recordings of the same individuals permitted explicit comparisons between the two calls. Advertisement call pulses averaged 2·4 more cycles (11·0-8·6) although pulses of both calls were basically similar as were durations and dominant frequencies. Pulse number, however, differed markedly, averaging 13·6 and 3·4 pulses for disturbance and advertisement calls respectively. Furthermore, disturbance calls were produced as a rapid series with an interpulse interval of 26-31 ms whereas advertisement call patterns were less stereotyped and ranged from <100 to 450 ms. Multicycle pulses distinguished U. canosai from other sympatric sciaenids.

  10. Justification for setting the individual animal genotype call rate threshold at eighty-five percent.

    Science.gov (United States)

    Purfield, D C; McClure, M; Berry, D P

    2016-11-01

    Data quality of SNP arrays impacts the accuracy and precision of downstream data analyses. One such quality control measure often imposed is a threshold on individual animal call rate. Different call rate thresholds have been applied across studies; little is known, however, about the impact of these thresholds on the quality of the genotype data. The objective of the present study was to investigate the effect of different call rate thresholds on the integrity of the genotypes but also to quantify the contribution of different factors to the variability in animal call rate. Data included 142,342 samples genotyped on a custom Illumina genotype panel from 141,591 dairy and beef cattle; the number of Illumina SNP on the panel was 14,371. The mean animal call rate across all samples was 99.09%; 487 animals had both a low call rate (call rate (≥99%) after resampling and regenotyping. Several factors were associated ( call rate including animal sex, the sampling herd, the date of genotyping, the genotyping plate, and the plate well. The genotype and allele concordance between the genotypes of the 487 low- and high-call rate individuals improved at a diminishing rate as mean animal call rate increased. Mean genotype and allele concordance rates of 0.987 and 0.997, respectively, existed when animal call rate was between 85 and 90%, increasing to 0.998 and 0.999, respectively, when animal call rate was between 95 and call rate animals increased when animal call rate improved; an allele concordance rate of 1.00 was achieved when animal call rate was between 85 and call rate animals improved as animal call rate increased; the mean genotype concordance rate of the imputed nonobserved SNP was 0.41 when animal call rate was call rate was between 95 and call rate was call rate threshold of 85%.

  11. Enhancing the Autonomous Use of CALL: A New Curriculum Model in EFL

    Science.gov (United States)

    Smith, Karen; Craig, Hana

    2013-01-01

    This action research study evaluates the effectiveness of a CALL Learner Autonomy (CALL LA) course at a Japanese university. The "C course" blends face-to-face instruction and independent study in a CALL environment. It aims to enhance learners' autonomous use of CALL in the acquisition of English as a Foreign Language (EFL). In…

  12. 17 CFR 21.00 - Preparation and transmission of information upon special call.

    Science.gov (United States)

    2010-04-01

    ... of information upon special call. 21.00 Section 21.00 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION SPECIAL CALLS § 21.00 Preparation and transmission of information upon special call. All information required upon special call shall be prepared in such form and manner and...

  13. 78 FR 70960 - Utah Resource Advisory Council Meeting/Conference Call

    Science.gov (United States)

    2013-11-27

    ... Bureau of Land Management Utah Resource Advisory Council Meeting/Conference Call AGENCY: Bureau of Land Management, Interior. ACTION: Notice of Meeting/Conference Call. SUMMARY: In accordance with the Federal Land... host a meeting/conference call. DATES: The BLM-Utah RAC will host a meeting/conference call on...

  14. Developing CALL to Meet the Needs of Language Teaching and Learning

    Science.gov (United States)

    Jiang, Zhaofeng

    2008-01-01

    This paper illustrates the advantages and disadvantages of CALL. It points out that CALL is influenced by traditional language teaching and learning approaches to some extent. It concludes that what is important in our university system is that CALL design and implementation should match the users' needs, since CALL is not always better than…

  15. An Analysis of Learner Autonomy and Learning Strateg ies in CALL Environment

    Institute of Scientific and Technical Information of China (English)

    何为芳

    2008-01-01

    In recent years,computer assisted language learning(CALL)increasingly attracts language learners'attentions with evident advantages.This paper introduces the basic concepts concerning CALL.learner autonomy and learning strategies,and tentatively proposes suggestions for developing language learners'strategies in CALL environment based on CALL situations in China.

  16. Linking CALL and SLA: Using the IRIS Database to Locate Research Instruments

    Science.gov (United States)

    Handley, Zöe; Marsden, Emma

    2014-01-01

    To establish an evidence base for future computer-assisted language learning (CALL) design, CALL research needs to move away from CALL versus non-CALL comparisons, and focus on investigating the differential impact of individual coding elements, that is, specific features of a technology which might have an impact on learning (Pederson, 1987).…

  17. 16 CFR 308.3 - Advertising of pay-per-call services.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Advertising of pay-per-call services. 308.3... Advertising of pay-per-call services. (a) General requirements. The following requirements apply to... of the call. (1) The provider of pay-per-call services shall clearly and conspicuously disclose...

  18. Analysis of Completion of Call with on-off Data Source in Wireless Networks

    Institute of Scientific and Technical Information of China (English)

    XUAN Xiao-ying; SHI Bing-xin; ZOU Ling

    2004-01-01

    In wireless network, call completion probability accounts for users' satisfaction since the admitted ongoing call may be interrupted during hand-off process or even stay in the same cell when dynamically allocating resource to calls because of the loss of resource. We focus on the relationship between call's completion probability and these interruptions and develop an analytical relationship model for homogeneous cellular networks based on probability analysis. Then assuming call's data source is modeled by on-off traffic model, a two dimensional Markov process is established to compute these blocking and dropping probabilities for call's completion probability. The impacts of different new call arrival rate, call's traffic characteristic, user's mobility, call's holding time and call's admission threshold on call's completion are evaluated and compared through numerical examples. These results show that call's completion reaches its maximum value if making no difference between hand-off call and new call in the case of light traffic load. But some resource should be reserved for the hand-off call in high traffic scenario. The analytical model provides a basis for helping to set the call admission threshold.

  19. ICT and the location of call centres: regional and local patterns

    NARCIS (Netherlands)

    Bruinsma, Frank; Rietveld, Piet; Beekman, Michiel

    2004-01-01

    One of the sectors that gained most of the boost in ICT developments is the call centres sector. The focus in this paper is on spatial diffusion patterns of call centres in the Netherlands. The number of call centres has increased rapidly in the last decade and it seems that impacts of call ce

  20. 49 CFR 198.37 - State one-call damage prevention program.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false State one-call damage prevention program. 198.37... REGULATIONS FOR GRANTS TO AID STATE PIPELINE SAFETY PROGRAMS Adoption of One-Call Damage Prevention Program § 198.37 State one-call damage prevention program. A State must adopt a one-call damage...