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Sample records for positive dp thymocytes

  1. Indian hedgehog (Ihh) both promotes and restricts thymocyte differentiation.

    Science.gov (United States)

    Outram, Susan V; Hager-Theodorides, Ariadne L; Shah, Divya K; Rowbotham, Nicola J; Drakopoulou, Ekati; Ross, Susan E; Lanske, Beate; Dessens, Johannes T; Crompton, Tessa

    2009-03-05

    We show that Indian Hedgehog (Ihh) regulates T-cell development and homeostasis in both fetal and adult thymus, controlling thymocyte number. Fetal Ihh(-/-) thymi had reduced differentiation to double-positive (DP) cell and reduced cell numbers compared with wild-type littermates. Surprisingly, fetal Ihh(+/-) thymi had increased thymocyte numbers and proportion of DP cells relative to wild type, indicating that Ihh also negatively regulates thymocyte development. In vitro treatment of thymus explants with exogenous recombinant Hedgehog protein promoted thymocyte development in Ihh(-/-) thymi but inhibited thymocyte development in Ihh(+/-), confirming both positive and negative regulatory functions of Ihh. Analysis of Rag(-/-)Ihh(+/-) thymi showed that Ihh promotes T-cell development before pre-T-cell receptor (pre-TCR) signaling, but negatively regulates T-cell development only after pre-TCR signaling has taken place. We show that Ihh is most highly expressed by the DP population and that Ihh produced by DP cells feeds back to negatively regulate the differentiation and proliferation of their double-negative progenitors. Thus, differentiation from double-negative to DP cell, and hence the size of the DP population, is dependent on the concentration of Ihh in the thymus. Analysis of Ihh conditional knockout and heterozygote adult mice showed that Ihh also influences thymocyte number in the adult.

  2. Retinoic acid signalling in thymocytes regulates T cell development

    DEFF Research Database (Denmark)

    Wendland, Kerstin; Sitnik, Katarzyna Maria; Kotarsky, Knut

    . Here, using a RA sensitive reporter mouse model, we demonstrate that endogenous RAR responses are induced in CD69+CD4+CD8lo and CD69+CD4+CD8+ thymocytes undergoing positive selection and lineage commitment, and continue to be present in both CD4+ and CD8+ single positive (SP) cells, with RA signaling...... further enhanced in recently generated CD69+ CD4+ SP cells. To address the potential biological significance of RA signaling in developing thymocytes, we evaluated T cell development in CD4Cre-dnRAR mice, where RA signaling is blocked in thymocytes from the CD4+CD8+ double positive (DP) stage onwards due...

  3. T cell factor-1 controls the lifetime of CD4+ CD8+ thymocytes in vivo and distal T cell receptor α-chain rearrangement required for NKT cell development.

    Directory of Open Access Journals (Sweden)

    Archna Sharma

    Full Text Available Natural killer T (NKT cells are a component of innate and adaptive immune systems implicated in immune, autoimmune responses and in the control of obesity and cancer. NKT cells develop from common CD4+ CD8+ double positive (DP thymocyte precursors after the rearrangement and expression of T cell receptor (TCR Vα14-Jα18 gene. Temporal regulation and late appearance of Vα14-Jα18 rearrangement in immature DP thymocytes has been demonstrated. However, the precise control of lifetime of DP thymocytes in vivo that enables distal rearrangements remains incompletely defined. Here we demonstrate that T cell factor (TCF-1, encoded by the Tcf7 gene, is critical for the extended lifetime of DP thymocytes. TCF-1-deficient DP thymocytes fail to undergo TCR Vα14-Jα18 rearrangement and produce significantly fewer NKT cells. Ectopic expression of Bcl-xL permits Vα14-Jα18 rearrangement and rescues NKT cell development. We report that TCF-1 regulates expression of RORγt, which regulates DP thymocyte survival by controlling expression of Bcl-xL. We posit that TCF-1 along with its cofactors controls the lifetime of DP thymocytes in vivo.

  4. Changes in thymocytes due to local irradiation of a portion of the maxilla in mice. A study of NKT cells and γδT cells

    International Nuclear Information System (INIS)

    Satho, Taigo; Tamasawa, Ken; Yosue, Takashi; Arai, Thiaki

    2000-01-01

    We have been investigating the changes of lymphocyte subsets in immune organs after head and neck irradiation. The aim of the present study was to determine the influence of local irradiation (10 Gy) of a portion of the maxilla on the thymocyte subsets, specifically NKT cells and γδT cells in mice. The thymocyte subsets were analyzed following irradiation using a fluorometer (anti-CD4, CD8, TCRαβ, TCRγδ and NK1.1 monoclonal antibodies) and the outcome compared with that obtained from no-irradiation groups. The following results were obtained: The total number of thymocytes showed a significant decrease one day and three days after irradiation. The absolute number of DN (double negative; CD4 - CD8 - ), CD4 + SP (CD4 + single positive; CD4 + CD8 - ) and CD8 + SP (CD8 + single positive; CD4 - CD8 + ) thymocytes showed a significant decrease one day after irradiation. The absolute number of DP (double positive; CD4 + CD8 + ) thymocytes showed a significant decrease one day and three days after irradiation. The absolute number of NKT and γδT cells showed a significant decrease one day after irradiation. In conclusion, the results suggest that NKT cells and γδT cells exhibit behavior which is the same as main stream thymocytes, except DP thymocytes. (author)

  5. Changes in thymocytes due to local irradiation of a portion of the maxilla in mice. A study of NKT cells and {gamma}{delta}T cells

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    Satho, Taigo; Tamasawa, Ken; Yosue, Takashi [Nippon Dental Univ., Tokyo (Japan); Arai, Thiaki

    2000-07-01

    We have been investigating the changes of lymphocyte subsets in immune organs after head and neck irradiation. The aim of the present study was to determine the influence of local irradiation (10 Gy) of a portion of the maxilla on the thymocyte subsets, specifically NKT cells and {gamma}{delta}T cells in mice. The thymocyte subsets were analyzed following irradiation using a fluorometer (anti-CD4, CD8, TCR{alpha}{beta}, TCR{gamma}{delta} and NK1.1 monoclonal antibodies) and the outcome compared with that obtained from no-irradiation groups. The following results were obtained: The total number of thymocytes showed a significant decrease one day and three days after irradiation. The absolute number of DN (double negative; CD4{sup -} CD8{sup -}), CD4{sup +} SP (CD4{sup +} single positive; CD4{sup +} CD8{sup -}) and CD8{sup +} SP (CD8{sup +} single positive; CD4{sup -} CD8{sup +}) thymocytes showed a significant decrease one day after irradiation. The absolute number of DP (double positive; CD4{sup +} CD8{sup +}) thymocytes showed a significant decrease one day and three days after irradiation. The absolute number of NKT and {gamma}{delta}T cells showed a significant decrease one day after irradiation. In conclusion, the results suggest that NKT cells and {gamma}{delta}T cells exhibit behavior which is the same as main stream thymocytes, except DP thymocytes. (author)

  6. Design and reliability analysis of DP-3 dynamic positioning control architecture

    Science.gov (United States)

    Wang, Fang; Wan, Lei; Jiang, Da-Peng; Xu, Yu-Ru

    2011-12-01

    As the exploration and exploitation of oil and gas proliferate throughout deepwater area, the requirements on the reliability of dynamic positioning system become increasingly stringent. The control objective ensuring safety operation at deep water will not be met by a single controller for dynamic positioning. In order to increase the availability and reliability of dynamic positioning control system, the triple redundancy hardware and software control architectures were designed and developed according to the safe specifications of DP-3 classification notation for dynamically positioned ships and rigs. The hardware redundant configuration takes the form of triple-redundant hot standby configuration including three identical operator stations and three real-time control computers which connect each other through dual networks. The function of motion control and redundancy management of control computers were implemented by software on the real-time operating system VxWorks. The software realization of task loose synchronization, majority voting and fault detection were presented in details. A hierarchical software architecture was planed during the development of software, consisting of application layer, real-time layer and physical layer. The behavior of the DP-3 dynamic positioning control system was modeled by a Markov model to analyze its reliability. The effects of variation in parameters on the reliability measures were investigated. The time domain dynamic simulation was carried out on a deepwater drilling rig to prove the feasibility of the proposed control architecture.

  7. Susceptibility of different subsets of immature thymocytes to apoptosis induced by anti-TCRmAb

    International Nuclear Information System (INIS)

    Li Hongmei; Zhong Renqian; Yu Jiaping; Kong Xiantao; Chen Weifeng

    2003-01-01

    To analysis the susceptibility of different subsets of immature mice thymocytes to apoptosis induced by anti-TCRmAbs in vitro apoptosis was induced in unfractionated mice thymocytes by anti-TCRmAb. In Vivo apoptosis was induced in BALB/c mice by anti-TCR mAb, and thymocytes were examined by FACS. Results showed that CD4 + CD8 + DP thymocytes and CD4 - CD8 + CD3 - thymocytes were equally sensitive to apoptosis after treatment with the anti-TCR mAb. In sharp contrast, the early migrants or precursor containing thymocytes which are CD4 - CD8 - CD3 - TN have a lower spontaneous apoptosis rate and were relatively resistant to the anti-TCR mAb. The findings showed a breakpoint in thymocyte sensitivity to apoptosis which occurs after the onset of CD4 - CD8 + CD3 expression, suggesting that susceptibility of thymocytes to apoptosis is developmentally regulated

  8. Alternative pathway for the development of Vα14+ NKT cells directly from CD4-CD8- thymocytes that bypasses the CD4+CD8+ stage.

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    Dashtsoodol, Nyambayar; Shigeura, Tomokuni; Aihara, Minako; Ozawa, Ritsuko; Kojo, Satoshi; Harada, Michishige; Endo, Takaho A; Watanabe, Takashi; Ohara, Osamu; Taniguchi, Masaru

    2017-03-01

    Although invariant V α 14 + natural killer T cells (NKT cells) are thought to be generated from CD4 + CD8 + double-positive (DP) thymocytes, the developmental origin of CD4 - CD8 - double-negative (DN) NKT cells still remains unresolved. Here we provide definitive genetic evidence obtained, through studies of mice with DP-stage-specific ablation of expression of the gene encoding the recombinase component RAG-2 (Rag2) and by a fate-mapping approach, that supports the proposal of the existence of an alternative developmental pathway through which a fraction of DN NKT cells with strong T-helper-type-1 (T H 1)-biased and cytotoxic characteristics develop from late DN-stage thymocytes, bypassing the DP stage. These findings provide new insight into understanding of the development of NKT cells and propose a role for timing of expression of the invariant T cell antigen receptor in determining the functional properties of NKT cells.

  9. Development of DP (Dynamic Positioning) to pull-in sub sea pipelines; Utilizacao de barcos de manuseio de ancoras operando com DP (Dynamic Positioning) para arraste de dutos submarinos

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    Oliveira, Manoel H.S. [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil); Galgoul, Elton C. [Suporte Engenharia, Luziania, GO (Brazil)

    2004-07-01

    Sub sea pipeline construction at oil and gas fields, with high concentration of on-bottom facilities, becomes more difficult when mooring operations and pipeline approach to a congested platform have to be performed. One method that has often been applied in Brazil is the so-called 'DP (Dynamic Positioning) pull-in', where PETROBRAS owned pipelay Barge, BGL-1, is moored away from the congested area, while a DP anchor handler pulls the rigid pipeline from BGL-1 to a target near the platform. The method was conceived to avoid mooring operations near the congested platforms as well as to minimize risks due to the pipeline initiation process. Inside the congested area the initiation with aid of a 'dead-man' anchor on the sea bottom, which would be a more conventional solution, becomes impossible in most cases. This paper will discuss the engineering work required to perform the 'DP pull-in' as well as show the operational steps, from the start-up to the final abandonment of the pipeline initiation head inside the target area. (author)

  10. Plxnd1 expression in thymocytes regulates their intrathymic migration while that in thymic endothelium impacts medullary topology

    Directory of Open Access Journals (Sweden)

    Young Il Choi

    2013-11-01

    Full Text Available An important role for plexinD1 in thymic development is inferred from studies of germline Plxnd1 knockout (KO mice where mislocalized CD69+ thymocytes as well as ectopic thymic subcapsular medullary structures were observed. Given embryonic lethality of the Plxnd1-/- genotype, fetal liver transplantation was employed in these prior analyses. Such embryonic hematopoietic reconstitution may have transferred Plxnd1 KO endothelial and/or epithelial stem cells in addition to Plxnd1 KO lymphoid progenitors, thereby contributing to that phenotype. Here we use Plxnd1flox/flox mice crossed to pLck-Cre, pKeratin14-Cre or pTek-Cre transgenic animals to create cell-type specific conditional knockout (CKO lines involving thymocytes (D1ThyCKO, thymic epithelium (D1EpCKO and thymic endothelium (D1EnCKO, respectively. These CKOs allowed us to directly assess the role of plexinD1 in each lineage. Loss of plexinD1 expression on double positive (DP thymocytes leads to their aberrant migration and cortical retention after TCR-mediated positive selection. In contrast, ectopic medulla formation is a consequence of loss of plexinD1 expression on endothelial cells, in turn linked to dysregulation of thymic angiogenesis. D1EpCKO thymi manifest neither abnormality. Collectively, our findings underscore the non-redundant roles for plexinD1 on thymocytes and endothelium, including the dynamic nature of medulla formation resulting from crosstalk between these thymic cellular components.

  11. Age-related synthesis of glucocorticoids in thymocytes

    International Nuclear Information System (INIS)

    Qiao Shengjun; Chen Liying; Okret, Sam; Jondal, Mikael

    2008-01-01

    Glucocorticoids (GCs) are primarily synthesized in the adrenal glands but an ectopic production has also been reported in the brain, the gastrointestinal tract and in thymic epithelial cells (TEC). Here we show that thymocytes express genes encoding for all enzymes required for de novo GC synthesis and produce the hormone as demonstrated by both a GC specific reporter assay and a corticosterone specific ELISA assay. Interestingly, GC synthesis is detectable in cells from young mice (4 weeks) and thereafter increases during aging (14-22 weeks) together with an increased gene expression of the rate-limiting enzymes StAR and CYP11A1. Hormone production occurred at a thymocyte differentiation stage characterized by being double positive for the CD4 and CD8 surface markers but was found to be unrelated to CD69 expression, a marker for thymocytes undergoing positive selection. No GC synthesis was found in resting or anti-CD3 activated CD4 and CD8 positive T cells isolated from the spleen. Thymocyte-derived GC had an anti-proliferative effect on a GR-transfected cell line and induced apoptosis in thymocytes. The age- and differentiation stage-related GC synthesis in thymocytes may play a role in the involution process that the thymus gland undergoes

  12. The DNA damage- and transcription-associated protein Paxip1 controls thymocyte development and emigration

    DEFF Research Database (Denmark)

    Callen, E.; Faryabi, R.B.; Daniel, Jeremy Austin

    2012-01-01

    Histone 3 lysine 4 trimethylation (H3K4me3) is associated with promoters of active genes and found at hot spots for DNA recombination. Here we have shown that PAXIP1 (also known as PTIP), a protein associated with MLL3 and MLL4 methyltransferase and the DNA damage response, regulates RAG......-mediated cleavage and repair during V(D)J recombination in CD4 CD8 DP thymocytes. Loss of PAXIP1 in developing thymocytes diminished Jα H3K4me3 and germline transcription, suppressed double strand break formation at 3' Jα segments, but resulted in accumulation of unresolved T cell receptor α-chain gene (Tcra......) breaks. Moreover, PAXIP1 was essential for release of mature single positive (SP) αβ T cells from the thymus through transcriptional activation of sphingosine-1-phosphate receptor S1pr1 as well as for natural killer T cell development. Thus, in addition to maintaining genome integrity during Tcra...

  13. Activins and inhibins: Novel regulators of thymocyte development

    International Nuclear Information System (INIS)

    Licona-Limon, Paula; Aleman-Muench, German; Chimal-Monroy, Jesus; Macias-Silva, Marina; Garcia-Zepeda, Eduardo A.; Matzuk, Martin M.; Fortoul, Teresa I.; Soldevila, Gloria

    2009-01-01

    Activins and inhibins are members of the transforming growth factor-β superfamily that act on different cell types and regulate a broad range of cellular processes including proliferation, differentiation, and apoptosis. Here, we provide the first evidence that activins and inhibins regulate specific checkpoints during thymocyte development. We demonstrate that both activin A and inhibin A promote the DN3-DN4 transition in vitro, although they differentially control the transition to the DP stage. Whereas activin A induces the accumulation of a CD8 + CD24 hi TCRβ lo intermediate subpopulation, inhibin A promotes the differentiation of DN4 to DP. In addition, both activin A and inhibin A appear to promote CD8 + SP differentiation. Moreover, inhibin α null mice have delayed in vitro T cell development, showing both a decrease in the DN-DP transition and reduced thymocyte numbers, further supporting a role for inhibins in the control of developmental signals taking place during T cell differentiation in vivo.

  14. Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro

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    Marvin Paulo Lins

    Full Text Available BACKGROUND: Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocytes and thymic epithelial cells (TEC in the presence of laminin. RESULTS: GH increased thymocyte adhesion on BSA-coated and further on laminin-coated surfaces. The number of migrating cells in laminin-coated membrane was higher in GH-treated thymocyte group. In both results, VLA-6 expression on thymocytes was constant. Also, treatment with GH enhanced laminin production by TEC after 24 h in culture. However, VLA-6 integrin expression on TEC remained unchanged. Finally, TEC/thymocyte co-culture model demonstrated that GH elevated absolute number of double-negative (CD4-CD8- and single-positive CD4+ and CD8+ thymocytes. A decrease in cell number was noted in double-positive (CD4+CD8+ thymocytes. CONCLUSIONS: The results of this study demonstrate that GH is capable of enhancing the migratory capacity of human thymocytes in the presence of laminin and promotes modulation of thymocyte subsets after co-culture with TEC.

  15. Co-ordinate expression of the pre-T-cell receptor complex and a novel immature thymocyte-specific antigen, IMT-1, during thymocyte development.

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    Tong, J J; Kishi, H; Nagata, T; Muraguchi, A

    1999-01-01

    Previously we described a monoclonal antibody (mAb) that reacted with a cell-surface antigen, immature thymocyte antigen-1 (IMT-1), which is expressed on thymocytes of late CD4- CD8- (double negative) to early CD4+ CD8+ (double positive) differentiation stages. In this study, we investigated the expression of IMT-1 on various cell lineages in thymus as well as in peripheral lymphoid organs. We found that IMT-1 is expressed on T-cell receptor (TCR)-betalo and TCR-deltalo thymocytes, but not on TCR-betahi, TCR-deltahi or natural killer (NK)1.1+ thymocytes, or on peripheral alpha beta or gamma delta T cells. We also investigated the kinetics of expression of IMT-1 during fetal thymocyte development and compared it with the expression of the pre-TCR complex, comprising CD3, pre-TCR-alpha (pTalpha) and TCR-beta. We found that expression of both was similar, starting at day 14.5 of gestation, peaking on day 16.5 and gradually decreasing thereafter. Furthermore, the expression of both IMT-1 and pTalpha was drastically reduced when DN thymocytes in recombination activating gene (RAG)-2-/- mice were challenged in vivo with anti-CD3 mAb. These results indicate that IMT-1 is expressed on not only immature thymocytes of alpha beta T-cell lineage but also on those of gamma delta T-cell lineage, and that the expression of IMT-1 and the pre-TCR complex is co-ordinately regulated during the alpha beta lineage thymocyte development.

  16. Post-irradiation thymocyte regeneration after bone marrow transplantation

    International Nuclear Information System (INIS)

    Boersma, W.J.A.

    1981-01-01

    Bone marrow cells were separated according to buoyant density, velocity sedimentation and cell surface charge. Fractionated (C3H x AKR)F 1 bone marrow cells were transplanted into lethally-irradiated C3H recipients. In all fractions, the CFUs content and the capacity to restore the thymus cell population were determined. For all the physical parameters tested, thymocyte progenitor cells show the same distribution as CFUs. The relationship between number of thymocyte progenitor cells and number of CFUs is dependent on density. Bone marrow progenitors of PHA responsive cells are of low buoyant density and show a distribution which resembles the distribution of the progenitors of Thy 1 positive cells. After transplantation of large numbers of bone marrow cells into irradiated mice, no significant change in the CFUs content of the thymus was observed. (author)

  17. Membranes as sensitive targets in thymocyte apoptosis

    International Nuclear Information System (INIS)

    Ramakrishnan, N.; McClain, D.E.; Catravas, G.N.

    1993-01-01

    The role of cellular membranes in thymocyte apoptosis has been examined. Trolox, a water soluble analogue of vitamin E and inhibitor of membrane damage, inhibits DNA fragmentation in thymocytes exposed to γ-radiation, and is most effective in inhibiting DNA fragmentation when added to cells within 30 min post-irradiation. Exposure to trolox only during irradiation did not prevent DNA fragmentation. Incubation of the irradiated cell suspension with trolox for 2h post-irradiation was sufficient to prevent DNA fragmentation measured at 24 h in irradiated cells, suggesting that trolox irreversibly inhibits a cellular lesion required for apoptosis. The induction of DNA fragmentation appears to be related to a concurrent, pronounced flow of Ca 2+ into the cell. At 3 h post-irradiation the amount of Ca 2+ in irradiated thymocytes was more than twice that of unirradiated thymocytes. Trolox treatment completely blocked the radiation-induced influx of Ca 2+ into the thymocytes. These results suggest that membrane damage is a critical lesion involved in DNA fragmentation in thymocyte apoptosis. (author)

  18. Study on the reactions dpdpπ+π- and dp → dnπ+ at 33 GeV/c in the hydrogen bubble chamber

    International Nuclear Information System (INIS)

    Aladashvili, B.S.; Glagolev, V.V.; Gorbunov, A.N.; Khairetdinov, K.U.; Lebedev, R.M.; Nioradze, M.S.; Saitov, I.S.; Streltsov, V.N.

    1975-01-01

    A narrow singularity is observed in the effective mass spectrum in the dpdpπ + π - and dp → dnπ + reactions at the incident deutron momentum of 3.3 GeV/c. The position and width of the singularity are (2,151+0.005) GeV and (13+6) MeV, respectively. The signal in the distribution of dπ + -mass at (2.294+0.005)GeV with the width of (47+13) MeV in reaction dp → dnπ + is identified as a kinematic effect

  19. Factors influencing the vaccinia-specific cytotoxic response of thymocytes from normal and chimeric mice

    International Nuclear Information System (INIS)

    Doherty, P.C.; Schwartz, D.H.; Bennink, J.R.; Korngold, R.

    1981-01-01

    Following adoptive transfer into irradiated recipients, thymocytes can be induced to respond strongly to vaccinia virus. High levels of cytotoxic T-lymphocyte (CTL) activity may be generated from thymus, but not from spleen, of 3-day-old mice. The capacity of thymocytes to differentiate into effector CTL tends to be lost with age. Some of this loss may reflect positive suppression: a single, low dose of cyclophosphamide allows the reemergence of responsiveness in at least one mouse strain. Thymocytes from [A leads to (A x B)F1] and [(A x B)F1 leads to A] chimeras show the response patterns that would by predicted from previous studies of lymph node and spleen cells. However, thymic function seems to be rapidly lost in the [A leads to (A x B)F1] Chimeras

  20. CD28 in thymocyte development and peripheral T cell activation in mice exposed to suspended particulate matter

    International Nuclear Information System (INIS)

    Drela, Nadzieja; Zesko, Izabela; Jakubowska, Martyna; Biernacka, Marzena

    2006-01-01

    The CD28:B7 signaling pathway is very important for the activity of mature peripheral T lymphocytes and thymocyte development. The proper development of thymocytes into mature single positive CD4 + and CD8 + T cells is crucial for almost all immune functions. In naturally occurring conditions, T cells maturation in the thymus is influenced by environmental agents. The expression of CD28 and the distribution of CD28 low/high thymocytes have been examined at various stages of thymocyte development in BALB/c mice exposed to air-suspended particulate matter (ASM). Acute exposure to ASM resulted in the decrease of CD28 expression in the total thymocyte population. The increase of the percentage of CD28 low and the decrease of CD28 high thymocytes were observed, which may account for the acceleration of thymocyte development under the conditions of elevated risk resulting from the exposure of animals to environmental xenobiotics. ASM exposure resulted in the increase of the level of proliferation of lymph node T cells induced by anti-CD3 and anti-CD28 monoclonal antibodies activation despite normal expression of CD28 molecule. In contrast, the level of proliferation of spleen T cells was lowered or normal dependently of the concentration of stimuli used for activation. Results of these studies demonstrate that acute exposure of mice to ASM can result in the progression of two contrasting processes in the immune system: upregulation of thymocyte development, which contributes to the maintenance of peripheral T cell pool, and over-activation of lymph node lymphocytes, which may lead to uncontrolled immunostimulation

  1. Notch3 is dispensable for thymocyte β-selection and Notch1-induced T cell leukemogenesis.

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    Sara Suliman

    Full Text Available Notch1 (N1 signaling induced by intrathymic Delta-like (DL ligands is required for T cell lineage commitment as well as self-renewal during "β-selection" of TCRβ⁺CD4⁻CD8⁻ double negative 3 (DN3 T cell progenitors. However, over-expression of the N1 intracellular domain (ICN1 renders N1 activation ligand-independent and drives leukemic transformation during β-selection. DN3 progenitors also express Notch3 (N3 mRNA, and over-expression of ligand-independent mutant N3 (ICN3 influences β-selection and drives T cell leukemogenesis. However, the importance of ligand-activated N3 in promoting β-selection and ICN1-induced T cell leukemogenesis has not been examined. To address these questions we generated mice lacking functional N3. We confirmed that DN3 progenitors express N3 protein using a N3-specific antibody. Surprisingly however, N3-deficient DN3 thymocytes were not defective in generating DP thymocytes under steady state conditions or in more stringent competition assays. To determine if N3 co-operates with N1 to regulate β-selection, we generated N1;N3 compound mutants. However, N3 deficiency did not exacerbate the competitive defect of N1⁺/⁻ DN3 progenitors, demonstrating that N3 does not compensate for limiting N1 during T cell development. Finally, N3 deficiency did not attenuate T cell leukemogenesis induced by conditional expression of ICN1 in DN3 thymocytes. Importantly, we showed that in contrast to N1, N3 has a low binding affinity for DL4, the most abundant intrathymic DL ligand. Thus, despite the profound effects of ectopic ligand-independent N3 activation on T cell development and leukemogenesis, physiologically activated N3 is dispensable for both processes, likely because N3 interacts poorly with intrathymic DL4.

  2. DP: Parameter Display Page Program

    International Nuclear Information System (INIS)

    Anderson, M.

    1994-01-01

    The Parameter Display Page program (DP) is a Motif/X11-based program to allow easily configured, dynamic device and process variable monitoring and manipulation in the EPICS environment. DP provides a tabular data format for interactive viewing and manipulation of device and process variable statistics, as well as formatted PostScript output to files and printers. DP understands and operates in two (unfortunately disjoint at this time) namespaces in the EPICS environment ''devices'' and ''process variables''. The higher level namespace of devices includes Composite and Atomic Devices registered via the Device Access server; the lower level (flat) namespace is that of normal Process Variables accessible via Channel Access

  3. Changes in plasma membrane structure upon irradiation on thymocytes

    International Nuclear Information System (INIS)

    Dreval', V.I.

    1993-01-01

    Thymocytes were irradiated with doses of 4 to 10 4 Gy. The binding of 1-anilinonaphtalene-8-sulphonate and Ca 2+ to plasma membranes; viscosity and lipid peroxidation; Stern-Folmer constant; and the number of Sh-groups of membrane proteins were determined. The structural changes in plasma membranes after irradiation of thymocytes were found to be cooperative

  4. Radiosensitivity of human lymphocytes and thymocytes

    International Nuclear Information System (INIS)

    Kwan, D.K.; Norman, A.

    1977-01-01

    The in vitro survival of human peripheral blood lymphocytes and thymocytes was measured 4 days following graded doses of γ radiation. Results indicate considerable heterogeneity among lymphocyte subpopulations with respect to radiosensitivity. Total T lymphocytes were characterized by rosette formation with neuraminidase-treated sheep red blood cells (nSRBC); early T (T/sub E/) cells, by early rosettes; and B cells, by their inability to form nSRBC rosettes. Late T (T/sub L/) cells were defined as T -- T/sub E/. Survival curves of T, T/sub E/, and B cells are biphasic. The radiosensitive and radioresistant components of T, T/sub E/, and B cells all have a D 0 of about 50 and 550 rad, respectively. B cells appeared to be slightly more radiosensitive than T cells. T/sub L/ cells and thymocytes, however, appeared to be homogeneous with respect to radiosensitivity, both having D 0 values of about 135 rad. The survival of T cells in mixed T and B cell cultures resembled that of separated T cells, suggesting that ionizing radiation has no significant effect on rosette formation. It also indicates that interactions of T and B cells do not significantly affect their radiation responses

  5. Thymocyte migration: an affair of multiple cellular interactions?

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    Savino W.

    2003-01-01

    Full Text Available Cell migration is a crucial event in the general process of thymocyte differentiation. The cellular interactions involved in the control of this migration are beginning to be defined. At least chemokines and extracellular matrix proteins appear to be part of the game. Cells of the thymic microenvironment produce these two groups of molecules, whereas developing thymocytes express the corresponding receptors. Moreover, although chemokines and extracellular matrix can drive thymocyte migration per se, a combined role for these molecules appears to contribute to the resulting migration patterns of thymocytes in their various stages of differentiation. The dynamics of chemokine and extracellular matrix production and degradation is not yet well understood. However, matrix metalloproteinases are likely to play a role in the breakdown of intrathymic extracellular matrix contents. Thus, the physiological migration of thymocytes should be envisioned as a resulting vector of multiple, simultaneous and/or sequential stimuli involving chemokines, adhesive and de-adhesive extracellular matrix proteins, as well as matrix metalloproteinases. Accordingly, it is conceivable that any pathological change in any of these loops may result in the alteration of normal thymocyte migration. This seems to be the case in murine infection by the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas' disease. A better knowledge of the physiological mechanisms governing thymocyte migration will provide new clues for designing therapeutic strategies targeting developing T cells.

  6. Novel leukocyte protein, Trojan, differentially expressed during thymocyte development.

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    Petrov, Petar; Motobu, Maki; Salmi, Jussi; Uchida, Tatsuya; Vainio, Olli

    2010-04-01

    "Trojan" is a novel cell surface protein, discovered from chicken embryonic thymocytes on the purpose to identify molecules involved in T cell differentiation. The molecule is predicted as a type I transmembrane protein having a Sushi and two fibronectin type III domains and a pair of intracellular phosphorylation sites. Its transcript expression is specific for lymphoid tissues and the presence of the protein on the surface of recirculating lymphocytes and macrophages was confirmed by immunofluorescence analysis. In thymus, about half of the double negative (CD4(-) CD8(-)) and CD8 single positive and the majority of CD4 single positive cells express Trojan with a relatively high intensity. However, only a minority of the double positive (CD4(+) CD8(+)) cells are positive for Trojan. This expression pattern, similar to that of some proteins with anti-apoptotic and function, like IL-7Ralpha, makes Trojan an attractive candidate of having an anti-apoptotic role. Copyright 2010 Elsevier Ltd. All rights reserved.

  7. The conveyor belt hypothesis for thymocyte migration: participation of adhesion and de-adhesion molecules.

    Science.gov (United States)

    Villa-Verde, D M; Calado, T C; Ocampo, J S; Silva-Monteiro, E; Savino, W

    1999-05-01

    Thymocyte differentiation is the process by which bone marrow-derived precursors enter the thymus, proliferate, rearrange the genes and express the corresponding T cell receptors, and undergo positive and/or negative selection, ultimately yielding mature T cells that will represent the so-called T cell repertoire. This process occurs in the context of cell migration, whose cellular and molecular basis is still poorly understood. Kinetic studies favor the idea that these cells leave the organ in an ordered pattern, as if they were moving on a conveyor belt. We have recently proposed that extracellular matrix glycoproteins, such as fibronectin, laminin and type IV collagen, among others, produced by non-lymphoid cells both in the cortex and in the medulla, would constitute a macromolecular arrangement allowing differentiating thymocytes to migrate. Here we discuss the participation of both molecules with adhesive and de-adhesive properties in the intrathymic T cell migration. Functional experiments demonstrated that galectin-3, a soluble beta-galactoside-binding lectin secreted by thymic microenvironmental cells, is a likely candidate for de-adhesion proteins by decreasing thymocyte interaction with the thymic microenvironment.

  8. The conveyor belt hypothesis for thymocyte migration: participation of adhesion and de-adhesion molecules

    Directory of Open Access Journals (Sweden)

    Villa-Verde D.M.S.

    1999-01-01

    Full Text Available Thymocyte differentiation is the process by which bone marrow-derived precursors enter the thymus, proliferate, rearrange the genes and express the corresponding T cell receptors, and undergo positive and/or negative selection, ultimately yielding mature T cells that will represent the so-called T cell repertoire. This process occurs in the context of cell migration, whose cellular and molecular basis is still poorly understood. Kinetic studies favor the idea that these cells leave the organ in an ordered pattern, as if they were moving on a conveyor belt. We have recently proposed that extracellular matrix glycoproteins, such as fibronectin, laminin and type IV collagen, among others, produced by non-lymphoid cells both in the cortex and in the medulla, would constitute a macromolecular arrangement allowing differentiating thymocytes to migrate. Here we discuss the participation of both molecules with adhesive and de-adhesive properties in the intrathymic T cell migration. Functional experiments demonstrated that galectin-3, a soluble ß-galactoside-binding lectin secreted by thymic microenvironmental cells, is a likely candidate for de-adhesion proteins by decreasing thymocyte interaction with the thymic microenvironment.

  9. The role of the maturation stage of thymocytes on the phytohemagglutinin and concanavalin A responses in thymocyte and lymph node lymphocyte cocultures in guinea pig. Effects of pretreatment of thymocytes with thymosin or levamisole.

    Science.gov (United States)

    Soppi, E

    1981-02-01

    Guinea pig thymocytes (TH) and lymph node lymphocytes (LNL) synergized optimally in both phytohemagglutinin (PHA) and concanavalin (Con A) responses in mixtures containing 0.3 x 10(6) TH and 0.2 x 10(6) LNL. Using discontinuous albumin gradient centrifugation thymocytes were separated into two subpopulations (F4 and F6) at different stages of maturation. Immature, PHA and Con A unresponsive F6 thymocytes synergized significantly only in the PHA response. More mature, PHA and Con A responsive F4 thymocytes cooperated well in the Con A response, but only a small synergy was observed in the PHA response. Pretreatment of the unfractionated thymocytes with the low concentration (0.05 microgram/ml) of thymosin decreased significantly their capacity to interact with LNL in both PHA and Con A responses. Preincubation of F4 thymocytes with the high concentration (200 microgram/ml) of thymosin increased the synergy in PHA response. All other combinations with thymosin or levamisole and thymocytes were ineffective on the mitogenic responses in the TH and LNL cocultures. Altogether, the results how that the thymocyte populations that induce synergy in PHA, or in (PHA and) Con A responses represent the two subpopulations of thymocytes with different maturation stages. The differential effects of the two concentrations of thymosin on the thymocytes support further the concept that the thymocytes synergizing in responses to both mitogens are more mature than those synergizing only PHA response. Thus, the ability of thymocytes to interact with LNL is dependent on the maturation stage of thymocytes, and can be utilized to study the differentiation of thymocytes.

  10. Deficiency of UV-induced excision repair in human thymocytes

    International Nuclear Information System (INIS)

    Gensler, H.L.; Lindberg, R.E.; Pinnas, J.L.; Jones, J.F.

    1985-01-01

    The capacity of human thymocytes and of differentiated lymphocytes circulating in peripheral blood to perform unscheduled DNA synthesis (a measure of nucleotide excision repair) after UV irradiation was measured by radioautographic analysis. Only 4% of immature T lymphocytes, but 68% of circulating lymphocytes exhibited unscheduled DNA synthesis. When UV sensitivity of peripheral blood lymphocytes and thymocytes from the same donor were compared, the thymocytes, in each case, were significantly more UV sensitive than were the circulating lymphocytes. Peripheral blood lymphocytes from subjects undergoing halothane and morphine anesthesia during surgery showed 56% less excision repair capacity than those from unanesthetized donors. The difference occurred in the number of cells capable of repair rather than in the extent of repair synthesis per cell. Ultraviolet-induced unscheduled DNA synthesis occurred in only 3% of the thymocytes removed from rats killed by cervical dislocation. Therefore, the deficiency of excision repair was observed in rat thymocytes which had not been affected by anesthesia or surgical trauma. The results indicate that immature T-cells are deficient in nucleotide excision repair whereas the majority of mature peripheral blood lymphocytes exhibit such repair. (author)

  11. T cell antigen receptor expression by subsets of Ly-2-L3T4- (CD8-CD4-) thymocytes

    DEFF Research Database (Denmark)

    Wilson, A; Ewing, T; Owens, T

    1988-01-01

    . No positive cells were detected among Ly-2-L3T4- thymocytes from V beta 8-negative SJL mice. In contrast to the adult thymus, Ly-2-L3T4- cells from embryonic CBA thymus lacked F23.1-positive cells. Subsets of adult CBA Ly-2-L3T4- thymocytes were separated to determine which expressed V beta 8. The major...... B2A2-M1/69- and Pgp-1+ all included strongly F23.1-positive cells. A minor subset, negative for most markers except Pgp-1 and presumed on the basis of this phenotype and some reconstitution studies to include the earliest intrathymic precursors, contained 28% F23.1-positive cells. However, no F.23...

  12. Gulf team delivers on DP drillship promise

    Energy Technology Data Exchange (ETDEWEB)

    Flatern, R von

    2001-06-01

    The technological achievements of the project by Amoco and BP to complete a deepwater subsea well in the Gulf of Mexico from a dynamically positioned (DP) vessel are described. In 2000, the dual activity drillship, Discoverer Enterprise (owned by Transocean Sedco Forex), completed the Nile well in the Viosca Knoll area and then the King Well in Mississippi Canyon Block 85. Stringent safety and environmental protection criteria imposed by Amoco and BP drove the design of the Dril-Quip subsea wellhead to ensure that the wellhead profile and connector coped with the worst case scenario. BP also specified a disconnect system that would secure the well in less than a minute. The SenTREE 7 and Commander telemetry systems developed by Schlumberger, the components of the work string and test work with the Nile well to ensure BP conditions were met and that the perforation and surge procedure proceeded successfully are explained. The time reduction achieved by using large DP drillships and future BP plans are outlined.

  13. Mechanism of chromatin degradation in thymocytes of irradiated rats

    International Nuclear Information System (INIS)

    Zotova, R.N.; Umanskij, S.R.; Tokarskaya, V.I.

    1983-01-01

    A biphase change in poly (ADP-ribose) polymerase activity of the thymocyte chromatin was observed after 10 Gy irradiation of rats: during the first minutes the incorporation of 14 C-NAD increased by 40% then started decreasing to make 110, 60 and 35% after 1, 2 and 3 h, respectively. Irradiation of rat thymus chromatin in vitro sharply decreased poly (ADP-ribose) polymerase activity. The possible role of changes in the poly (ADP-ribose) synthesis in the activation of nuclear Ca/Mg-dependent endonuclease and in the postirradiation degradation of the thymocyte chromatin is discussed

  14. In vitro evidence for participation of DEC-205 expressed by thymic cortical epithelial cells in clearance of apoptotic thymocytes.

    NARCIS (Netherlands)

    Small, M; Kraal, G.

    2003-01-01

    Binding of apoptotic cells was compared after incubation of thymocytes with two clones of murine thymic stromal cells to which CD4(+)/CD8(+) thymocytes attach. With the BA/10, but not the BA/2, clone, thymocytes with apoptotic morphology were bound irreversibly. These tightly bound thymocytes were

  15. Clonally Expanding Thymocytes Having Lineage Capability in Gamma-Ray-Induced Mouse Atrophic Thymus

    International Nuclear Information System (INIS)

    Yamamoto, Takashi; Morita, Shin-ichi; Go, Rieka; Obata, Miki; Katsuragi, Yoshinori; Fujita, Yukari; Maeda, Yoshitaka; Yokoyama, Minesuke; Aoyagi, Yutaka; Ichikawa, Hitoshi; Mishima, Yukio; Kominami, Ryo

    2010-01-01

    Purpose: To characterize, in the setting of γ-ray-induced atrophic thymus, probable prelymphoma cells showing clonal growth and changes in signaling, including DNA damage checkpoint. Methods and Materials: A total of 111 and 45 mouse atrophic thymuses at 40 and 80 days, respectively, after γ-irradiation were analyzed with polymerase chain reaction for D-J rearrangements at the TCRβ locus, flow cytometry for cell cycle, and Western blotting for the activation of DNA damage checkpoints. Results: Limited D-J rearrangement patterns distinct from normal thymus were detected at high frequencies (43 of 111 for 40-day thymus and 21 of 45 for 80-day thymus). Those clonally expanded thymocytes mostly consisted of CD4 + CD8 + double-positive cells, indicating the retention of lineage capability. They exhibited pausing at a late G1 phase of cell cycle progression but did not show the activation of DNA damage checkpoints such as γH2AX, Chk1/2, or p53. Of interest is that 17 of the 52 thymuses showing normal D-J rearrangement patterns at 40 days after irradiation showed allelic loss at the Bcl11b tumor suppressor locus, also indicating clonal expansion. Conclusion: The thymocytes of clonal growth detected resemble human chronic myeloid leukemia in possessing self-renewal and lineage capability, and therefore they can be a candidate of the lymphoma-initiating cells.

  16. Thymocyte apoptosis in response to low-dose radiation

    International Nuclear Information System (INIS)

    Shu-Zheng, Liu; Ying-Chun, Zhang; Ying, Mu; Xu, Su; Jian-Xiang, Liu

    1996-01-01

    Thymocyte apoptosis was assessed by counting apoptotic bodies with flow cytometry (FCM) and measuring DNA fragmentation with fluorescence spectrophotometry (FSP). J-shaped dose-response curves were obtained after both whole-body irradiation (WBI) of mice and in vitro irradiation of EL4 cells with doses ranging from 0.025 to 4 Gy X-rays. There was a significant reduction of apoptosis rate to below control level with doses within 0.2 Gy, and a dose-dependent increase in apoptosis with doses above 0.5 Gy. When thymocytes were cultured 24 h after WBI with 75 mGy X-rays in complete RPMI 1640 medium, a reduction in apoptosis was observed in the course of incubation for 72 h, and the presence of Con A in the medium accentuated this reduction in a dose- and time-dependent manner. The implications of these observations and the possible molecular mechanisms for future studies are proposed

  17. Ontogeny of the thymus in a teleost fish, Cyprinus carpio L.: developing thymocytes in the epithelial microenvironment.

    Science.gov (United States)

    Romano, N; Taverne-Thiele, A J; Fanelli, M; Baldassini, M R; Abelli, L; Mastrolia, L; Van Muiswinkel, W B; Rombout, J H

    1999-01-01

    A monoclonal antibody, WCL9, specific for membrane molecules of a thymocyte subpopulation was used to detect these cells in situ during the ontogeny of thymus. Cryo-sections revealed WCL9+ cells in the rudiment of the thymus (day 4 post fertilization); thereafter, the positive cells were observed exclusively in the cortex from the first appearance of thymic regionalization (week 4 post fertilization) until adult age. Whole-mount immunostaining of the thymus with WCL9 revealed the three-dimensional structure of the cortex by specific staining. The presence and distribution of apoptotic cells during thymus development was studied by in situ end-labelling of fragmented DNA. From week 4 post fertilization onwards, apoptotic cells were more frequently detected in the cortex than medulla, suggesting a continuous selection of thymocytes in the cortex. Ultrastructural studies confirmed the presence of numerous cortical apoptotic cells inside macrophages. Electron microscopy provided evidence for the existence of epithelial heterogeneity in the thymus. During the ontogeny, the differentiation of epithelial cells was followed from the first weeks until the juvenile age. Cell types were classified on the basis of their localization and cytological characteristics as: i) limiting epithelial cells located in subcapsular, perivascular and peritrabecular zones; ii) reticular epithelial cells situated in medullary and cortical zones; iii) nurse-like cells at the border between the cortex and medulla, iiii) Hassall's body-like structures localized in the medulla. This study could suggest the occurrence of a wide range of lympho-epithelial interactions throughout thymocytes differentiation.

  18. Mechanism of chromatin degradation in thymocytes of irradiated rats

    International Nuclear Information System (INIS)

    Nikonova, L.V.; Nelipovich, P.A.; Umanskij, S.R.

    1983-01-01

    Chromatin digestion in isolated thymocyte nuclei with DNAase I, micrococcal nuclease and nuclease from Serratia marcescens was studied. It was shown that 3 h after irradiation (10 Gy), the kinetics of accumulation of acid soluble and salt soluble products of DNA degradation, caused by exogenous nucleases, remains unchanged. The administration of cycloheximide does not influence the sensitivity of chromatin to DNAase I and somewhat increases the rate of salt soluble products formation upon the nuclease from S, marcescens treatment

  19. Retinoic acid signalling in thymocytes regulates T cell development

    DEFF Research Database (Denmark)

    Wendland, Kerstin; Sitnik, Katarzyna Maria; Kotarsky, Knut

    in the regulatory regions of targetgenes. RA has been reported to play a direct role in regulating multiple aspects of peripheralT cell responses1, but whether endogenous RA signalling occurs in developingthymocytes and the potential impact of such signals in regulating T cell developmentremains unclear. To address......RARα. This blocks RA signalling in developing thymocytes from the DN3/4 stageonwards and thus allows us to study the role of RA in T cell development...

  20. Thymocyte apoptosis induced by p53-dependent and independent pathways

    International Nuclear Information System (INIS)

    Clarke, A.R.; Purdie, C.A.; Harrison, D.J.; Morris, R.G.; Bird, C.C.; Hooper, M.L.; Wyllie, A.H.

    1993-01-01

    The authors studied the dependence of apoptosis on p53 expression in cells from the thymus cortex. Short-term thymocyte cultures were prepared from mice constitutively heterozygous or homozygous for a deletion in the p53 gene introduced into the germ line after gene targeting. Wild-type thymocytes readily undergo apoptosis after treatment with ionizing radiation, the glucocorticoid methylprednisolone, or etoposide (an inhibitor of topoisomerase II), or after Ca 2+ -dependent activation by phorbol ester and a calcium ionophore. In contrast, homozygous null p53 thymocytes are resistant to induction of apoptosis by radiation or etoposide, but retain normal sensitivity to glucocorticoid and calcium. The time-dependent apoptosis that occurs in untreated cultures is unaffected by p53 status. Cells heterozygous for p53 deletion are partially resistant to radiation and etoposide. Results show that p53 exerts a significant and dose-dependent effect in the initiation of apoptosis, but only when it is induced by agents that cause DNA-strand breakage. (Author)

  1. Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus Infection

    Directory of Open Access Journals (Sweden)

    Maria C. Lopez

    2005-01-01

    Full Text Available In this study, mouse MLN cells and thymocytes from advanced stages of LP-BM5 retrovirus infection were studied. A decrease in the percentage of IL-7+ cells and an increase in the percentage of IL-16+ cells in the MLN indicated that secretion of these cytokines was also altered after LP-BM5 infection. The percentage of MLN T cells expressing IL-7 receptors was significantly reduced, while the percentage of MLN T cells expressing TNFR-p75 and of B cells expressing TNFR-p55 increased. Simultaneous analysis of surface markers and cytokine secretion was done in an attempt to understand whether the deregulation of IFN-Υ secretion could be ascribed to a defined cell phenotype, concluding that all T cell subsets studied increased IFN-Υ secretion after retrovirus infection. Finally, thymocyte phenotype was further analyzed trying to correlate changes in thymocyte phenotype with MLN cell phenotype. The results indicated that the increase in single positive either CD4+CD8- or CD4- CD8+ cells was due to accumulation of both immature (CD3- and mature (CD3+ single positive thymocytes. Moreover, single positive mature thymocytes presented a phenotype similar to the phenotype previously seen on MLN T cells. In summary, we can conclude that LP-BM5 uses the immune system to reach the thymus where it interferes with the generation of functionally mature T cells, favoring the development of T cells with an abnormal phenotype. These new T cells are activated to secrete several cytokines that in turn will favor retrovirus replication and inhibit any attempt of the immune system to control infection.

  2. S1P lyase in thymic perivascular spaces promotes egress of mature thymocytes via up-regulation of S1P receptor 1.

    Science.gov (United States)

    Maeda, Yasuhiro; Yagi, Hideki; Takemoto, Kana; Utsumi, Hiroyuki; Fukunari, Atsushi; Sugahara, Kunio; Masuko, Takashi; Chiba, Kenji

    2014-05-01

    Sphingosine 1-phosphate (S1P) and S1P receptor 1 (S1P1) play an important role in the egress of mature CD4 or CD8 single-positive (SP) thymocytes from the thymus. Fingolimod hydrochloride (FTY720), an S1P1 functional antagonist, induced significant accumulation of CD62L(high)CD69(low) mature SP thymocytes in the thymic medulla. Immunohistochemical staining using anti-S1P1 antibody revealed that S1P1 is predominantly expressed on thymocytes in the thymic medulla and is strongly down-regulated even at 3h after FTY720 administration. 2-Acetyl-4-tetrahydroxybutylimidazole (THI), an S1P lyase inhibitor, also induced accumulation of mature SP thymocytes in the thymic medulla with an enlargement of the perivascular spaces (PVS). At 6h after THI administration, S1P1-expressing thymocytes reduced partially as if to form clusters and hardly existed in the proximity of CD31-expressing blood vessels in the thymic medulla, suggesting S1P lyase expression in the cells constructing thymic medullary PVS. To determine the cells expressing S1P lyase in the thymus, we newly established a mAb (YK19-2) specific for mouse S1P lyase. Immunohistochemical staining with YK19-2 revealed that S1P lyase is predominantly expressed in non-lymphoid thymic stromal cells in the thymic medulla. In the thymic medullary PVS, S1P lyase was expressed in ER-TR7-positive cells (reticular fibroblasts and pericytes) and CD31-positive vascular endothelial cells. Our findings suggest that S1P lyase expressed in the thymic medullary PVS keeps the tissue S1P concentration low around the vessels and promotes thymic egress via up-regulation of S1P1.

  3. Genetic control of susceptibility to apoptosis of thymocytes

    International Nuclear Information System (INIS)

    Mori, N.; Okumoto, M.; Morimoto, J.; Imai, S.; Matsuyama, T.; Takamori, Y.; Yagasaki, O.

    1992-01-01

    Genetic control of the susceptibility of thymocytes to radiation-induced apoptosis in mice was investigated by counting dead cells in a selected area of thymic cortex on histological specimens after whole-body X-irradiation. The number of dead cells increased almost linearly with doses in BALB/cHeA and STS/A mice. However, dead cell counts in BALB/cHeA mice were more than twice those in STS/A mice at each dose. C57BL/6N and B10.BR mice exhibited a sensitive phenotype similar to BALB/cHeA mice, while C3H/HeMsNrs and NFS/N mice showed a resistant phenotype similar to STS/A mice. Sex difference in the susceptibility of thymocytes to cell death was not recognized in BALB/cHeA and STS/A mice. Resistance was dominant over susceptibility in the progenies of reciprocal crosses between the two strains, indicating an autosomal inheritance. The segregation ratio of susceptible to resistant phenotype in the backcrosses of (BALB/cHeA X STS/A)F 1 with BALB/cHeA was not significantly different from 1 : 1 and all backcrosses of (BALB/cHeA X STS/A)F 1 with STS/A exhibited a resistant phenotype. The results demonstrated that the difference in the susceptibility of thymocytes to radiation-induced apoptosis in the two strains of mice is due to one major autosomal allele. (author)

  4. Biophysical studies of irradiated thymocytes. 1. Surface changes

    Energy Technology Data Exchange (ETDEWEB)

    Sungurov, A Yu; Tokalov, S V; Petrov, Yu P; Sharlaeva, T M

    1985-08-15

    In order to study postirradiation changes in thymus lymphocyte surface, a number of biophysical analytical methods was used: the cell two-partition method, the physical adhesion method, fluorescence intensity and binding parameters of negatively charged ANS probe. Reduction of cell distribution factor in two-phase system and adhesion of thymocytes to cotton 1 hour after irradiation, as well as abrupt change in parameters of binding the probe in the interval of 3-4 hours after X-ray irradiation at the dose of 4 Gy are demonstrated.

  5. Effects of terrestrial and marine organic matters on deposition of dechlorane plus (DP) in marine sediments from the Southern Yellow Sea, China: Evidence from multiple biomarkers

    International Nuclear Information System (INIS)

    Wang, Guoguang; Peng, Jialin; Hao, Ting; Feng, Lijuan; Liu, Qiaoling; Li, Xianguo

    2017-01-01

    As an emerging halogenated organic contaminant, Dechlorane Plus (DP) was scarcely reported in marine environments, especially in China. In this work, 35 surface sediments and a sediment core were collected across the Southern Yellow Sea (SYS) to comprehensively explore the spatio-temporal distribution and possible migration pathway of DP. DP concentrations ranged from 14.3 to 245.5 pg/g dry weight in the surface sediments, displaying a seaward increasing trend with the high levels in the central mud zone. This spatial distribution pattern was ascribed to that fine particles with the elevated DP levels were preferentially transported to the central mud zone under hydrodynamic forcing and/or via long-range atmospheric transportation and deposition. DP concentrations in sediment core gradually increased from the mid-1950s to present, which corresponded well with the historical production and usage of DP, as well as the economic development in China. Significantly positive correlation between DP and total organic carbon (TOC) in both surface sediments and sediment core indicated TOC-dependent natural deposition of DP in the SYS. We used multiple biomarkers, for the first time, to explore the potential effects of terrestrial and marine organic matters (TOM and MOM) on DP deposition. The results showed that competition may occur between TOM and MOM for DP adsorption, and MOM was the predominant contributor in controlling DP deposition in the marine sediments from the SYS. - Highlights: • Effects of TOM and MOM on DP deposition were first explored by multi-biomarkers. • Hydrodynamic forcing and atmospheric deposition were responsible for DP in the SYS. • MOM was the predominant contributor in controlling DP deposition to sediments in the SYS. • Competition may occur between TOM and MOM for DP adsorption. - This study was the first attempt to comprehensively explore the effects of TOM and MOM on DP deposition in marine sediments from the SYS.

  6. Kinetic analysis of thymocyte attachment to thymus stromal cells in culture by using phase-contrast and scanning electron microscopy

    International Nuclear Information System (INIS)

    LaRochelle, G.G.; Jones, K.H.

    1989-01-01

    Direct cellular contact between thymocytes and thymus stromal cells within the thymus appears to contribute to the maturation of thymocytes. Thymocyte-stromal cell complexes, formed in vivo, have been isolated by others and postulated to play a role in T-cell differentiation. These previous studies have been hampered, however, by a time-consuming isolation procedure from which only small numbers of these complexes are recovered. We have examined a model to study thymocyte-stromal cell complexes in vitro in which thymocytes are added to primary cultures of thymus stromal cells. In the present study, we found that thymocytes were histotypically selective in their attachment to thymus stromal cells. We also investigated the kinetics of thymocyte attachment to these thymus stromal cells. Cultures were examined at selected time intervals from 5 min through 3 days of incubation. Thymocyte attachment to stromal cells was a biphasic interaction, with maximum surface attachment at 15 min of cocultivation, followed by migration of thymocytes into the cultures. Morphological studies were confirmed by using 3 H-leucine-labeled thymocytes and liquid scintigraphy. With increased time in culture, thymocytes became amoeboid and migrated between the layers of stromal cells where thymocyte mitotic figures were seen at 4 and 8 hr. In some cases it appeared that stromal cells, which often grew two to three cell layers deep, played an active role in enclosing thymocytes within the cultures. Large numbers of viable thymocytes were observed in the cultures at 24 hr. The number of thymocytes then decreased progressively on days 2 and 3, when relatively few were found within the layers of the culture

  7. Stimulation of respiration in rat thymocytes induced by ionizing radiation

    International Nuclear Information System (INIS)

    Gudz, T.I.; Pandelova, I.G.; Novgorodov, S.A.

    1994-01-01

    The effect of X irradiation on the respiration of rat thymocytes was studied. An increase in the rate of O 2 uptake was observed 1 h after cells were irradiated with doses of 6-10 Gy. The radiation-induced increase in respiration could be blocked by oligomycin, an inhibitor of mitochondrial ATP synthase, suggesting control by increased cytoplasmic ATP turnover. The stimulation of respiration was not associated with changes in the activity of mitochondrial electron transfer enzymes or permeability of the inner membrane. Several inhibitors of processes which used ATP were screened for their effects on the basal respiration rate and on the radiation response. In irradiated thymocytes, an enhancement of inhibition of respiration by ouabain, La 3+ and cycloheximide was observed. These results indicate that the radiation-induced stimulation of respiration is due to changes in ion homeostasis and protein synthesis. The effect of X irradiation was shown to be independent of the redox status of nonprotein thiols and was not associated with detectable changes in some products of lipid peroxidation. The radiation-induced decrease in activity of superoxide dismutase suggests free radical involvement in deleterious effects of radiation. 43 refs., 2 figs., 3 tabs

  8. Methods of Thrust Allocation in a DP Simulation System of Maritime University of Szczecin

    Directory of Open Access Journals (Sweden)

    Zalewski Paweł

    2016-12-01

    Full Text Available Vessels conducting dynamic positioning (DP operations are usually equipped with thruster configurations that enable generation of resultant force and moment in any direction. These configurations are deliberately redundant in order to reduce the consequences of thruster failures and increase the safety. On such vessels a thrust allocation system must be used to distribute the control actions determined by the DP controller among the thrusters. The optimal allocation of thrusters′ settings in DP systems is a problem that can be solved by several convex optimization methods depending on criteria and constraints used. The paper presents linear programming (LP and quadratic programming (QP methods adopted in DP control model which is being developed in Maritime University of Szczecin for ship simulation purposes.

  9. Restoration of adenosine deaminase-deficient human thymocyte development in vitro by inhibition of deoxynucleoside kinases.

    Science.gov (United States)

    Joachims, Michelle L; Marble, Patrick A; Laurent, Aletha B; Pastuszko, Peter; Paliotta, Marco; Blackburn, Michael R; Thompson, Linda F

    2008-12-01

    Mutations in the gene encoding adenosine deaminase (ADA), a purine salvage enzyme, lead to immunodeficiency in humans. Although ADA deficiency has been analyzed in cell culture and murine models, information is lacking concerning its impact on the development of human thymocytes. We have used chimeric human/mouse fetal thymic organ culture to study ADA-deficient human thymocyte development in an "in vivo-like" environment where toxic metabolites accumulate in situ. Inhibition of ADA during human thymocyte development resulted in a severe reduction in cellular expansion as well as impaired differentiation, largely affecting mature thymocyte populations. Thymocyte differentiation was not blocked at a discrete stage; rather, the paucity of mature thymocytes was due to the induction of apoptosis as evidenced by activation of caspases and was accompanied by the accumulation of intracellular dATP. Inhibition of adenosine kinase and deoxycytidine kinase prevented the accumulation of dATP and restored thymocyte differentiation and proliferation. Our work reveals that multiple deoxynucleoside kinases are involved in the phosphorylation of deoxyadenosine when ADA is absent, and suggests an alternate therapeutic strategy for treatment of ADA-deficient patients.

  10. Thymic lymphocytes. III. Cooperative phenomenon in the proliferation of thymocytes under Con A stimulation.

    Science.gov (United States)

    Papiernik, M; Jacobson, J B

    1986-01-01

    In the present paper, the response of thymocytes to Con A is analyzed in terms of a cooperative phenomenon between medullary thymocytes, cortical thymocytes, thymic accessory cells, and interleukin 2. Medullary thymocytes respond spontaneously to Con A and produce IL-2. The addition of exogenously produced IL-2 enhances their proliferation. Small numbers of cortical (PNA+) thymocytes do not respond to Con A, even in the presence of IL-2-containing supernatant. By increasing the number of PNA+ cells per well, sensitivity to Con A and IL-2 appears. This response may be linked either to the increase in a minor PNA+-responding population and/or to the enhanced contamination by medullary thymocytes and macrophages in non-responding PNA+ thymocyte population. In this hypothesis, either the contaminating cells respond by themselves and/or cooperate with PNA+ cells to induce their proliferation. Coculture of non-responding low numbers of PNA+ thymocytes with Con A- and IL-2-containing supernatant in the presence of PNA- cells containing thymic medullary thymocytes and macrophages always produces a higher response than that of each individual population. These results show that a cooperative phenomenon occurs in the cocultures of PNA+ and PNA- thymic cells. We can show using PNA+ and PNA- thymocytes with different Thy 1 alleles, that indeed both PNA+ and populations participate PNA-thymocytes with different Thy 1 alleles, that indeed both PNA+ and PNA- populations participate in the generation of proliferating cells. We can demonstrate, by lysis experiments with monoclonal antibodies and complement that at the end of coculture, most of the proliferating cells are Lyt 1+, and part are Lyt 2+ or L3T4+. We discuss the fact that the phenotype of the cells after activation does not allow us to deduce the phenotype of their precursors. Lysis of Ia+ cells prior to coculture, reduces the level of the proliferative response but does not modify the percentage of cooperation produced

  11. [Effect of irradiation on the degradation of rat thymocyte chromatin].

    Science.gov (United States)

    Tsudzevich, B O; Parkhomets', Iu P; Andriĭchuk, T R; Iurkina, V V

    1998-01-01

    Genome instability of adaptive nature is formed under the experimental influence on a cell. Under critical conditions, strategy of organism is to damage the cells that cannot be restored and controlled by including the program of apoptosis. The ordered internucleosomal DNA degradation is considered to be one of the proof attributes of immunocompetent cell apoptosis. We investigated the effects of various doses of irradiation on the thymocytes chromatine fragmentation in 1,2,3 hours after a single X-ray exposure or after chronic influence in conditions of Chernobyl research base. By the means of electrophoresis in agarose and judging by polydeoxyribonucleotides accumulation we observed the "ladder pattern" of degradation in 3 hr after single 1 Gr irradiation (the smallest dose displaying the effect). We suppose that the influence of both chronic low-intensity irradiation taking place in Chernobyl and single X-ray exposure result in intensifying of DNA fragmentation in the cells of immunocompetent organs.

  12. Restriction fragment length polymorphism of the HLA-DP subregion and correlations to HLA-DP phenotypes

    International Nuclear Information System (INIS)

    Hyldig-Nielsen, J.J.; Morling, N.; Oedum, N.; Ryder, L.P.; Platz, P.; Jakobsen, B.; Svejgaard, A.

    1987-01-01

    The restriction fragment length polymorphism (RFLP) of the class II HLA-DP subregion of the major histocompatibility complex (MHC) of humans has been unraveled by Southern blotting using DP/sub α/ and DP/sub β/ probes in a study of 46 unrelated individuals with known HLA-DP types. Contrary to earlier preliminary findings with a limited number of enzymes, the RFLP appears to be quite extensive both with the DP/sub β/ (14 different DNA markers defined by individual fragments or clusters thereof) and the DP/sub α/ (8 markers) probes, especially when enzyme recognizing only four base pairs were used. A few markers were absolutely or strongly associated with individual DP antigens, whereas most were associated with two or more DP antigens as defined by primed lymphocyte typing. Thus, Southern blotting seems feasible for typing for most DP determinants by specific fragments or subtraction between the various more broadly reactive DNA markers, and the RFLP provides further information on the DP subregion in addition to that provided by primed lymphocyte typing. In two recombinant families, the DP/sub β/ and DP/sub α/ DNA markers segregated with DP antigens, whereas the DR/sub β/, DQ/sub β/, DQ/sub α/, and DX/sub α/ markers followed the DR and DQ antigens

  13. Toxicity of organotin compounds : IV. Impairment of energy metabolism of rat thymocytes by various dialkyltin compounds

    NARCIS (Netherlands)

    Penninks, A.H.

    2006-01-01

    Isolated thymocytes were incubated with various carbohydrates in the presence of dimethyltindichloride (DMTC), diethyltindichloride (DETC), di-n-butyltindichloride (DBTC), and di-n-octyltindichloride (DOTC) and the substrate conversion, oxygen consumption, and cell viability were measured. All the

  14. Effect of syngeneic thymocytes on proliferation of the small intestinal epithelium in mice

    International Nuclear Information System (INIS)

    Shmakov, A.N.; Aparovich, G.G.; Trufakin, V.A.

    1986-01-01

    This paper describes the study of the action of syngeneic thymocytes on proliferation of the epithelium of the mouse small intestine. The mice were injected with 3 H-thymidine in the experiments. Under the experimental conditions presented here, syngeneic thymocytes can reduce the number of DNA-synthesizing cells in the intestinal epithelium, causing narrowing of the zone of proliferation and enlargement of the zone of differentiation of the enterocytes

  15. Cell surface appearance of unexpected host MHC determinants on thymocytes from radiation bone marrow chimeras

    International Nuclear Information System (INIS)

    Sharrow, S.O.; Mathieson, B.J.; Singer, A.

    1981-01-01

    The phenotypic appearance of cell surface antigens on murine thymocytes from long-term radiation bone marrow chimeras was analyzed using indirect immunofluorescence and flow microfluorometry. Cells maturing in the thymi of these mice were typed for MHC (Kk, I-Ak, H-2b, Kb, and Ib) and non-MHC (Lty 1, Ly 9, and TL) determinants. All cells were of donor origin as determined by non-MHC (Ly) phenotype in P1 leads to P2, P1 x P2 leads to P1, and P1 leads to P2 radiation chimeras. In contrast, the MHC phenotypes of these thymocytes were markedly affected by the host environment. Specifically, H-2 and I-A determinants of both parental phenotypes were detected on thymocytes from P1 leads to P1 x P2 chimeras; I-A determinants of host phenotype were present, whereas I-A determinants of donor phenotype were reduced on thymocytes from P1 x P2 leads to P1 chimeras; and thymocytes from P1 leads to P2 chimeras possessed H-2 and I-A determinants of host phenotype but showed reduction of donor I-A phenotype determinants. The appearance of host cell surface H-2 and I-A determinants on thymocytes from chimeras closely parallels the functional recognition of MHC determinants by T cells from chimeric mice and thus may be significantly related to the development of the self-recognition repertoire by maturing T cells

  16. Human thymic epithelial cells express functional HLA-DP molecules

    DEFF Research Database (Denmark)

    Jørgensen, A; Röpke, C; Nielsen, M

    1996-01-01

    T lymphocytes, we examined whether human thymic epithelial cells (TEC) expressed HLA-DP molecules. We present evidence that TEC obtained from short time culture express low but significant levels of HLA-DP molecules. The expression of HLA-DP molecules was comparable to or higher than the expression...... of HLA-DP allospecific primed lymphocyte typing (PLT) CD4 T cell lines. IFN-gamma treatment strongly upregulated the HLA-DP allospecific PLT responses whereas other PLT responses remained largely unchanged. In conclusion, these data indicate that human thymus epithelial cells express significant levels...

  17. Profibus DP : implementering av Profibus DP i en AVR-mikrokontroller

    OpenAIRE

    Junell, Andreas

    2012-01-01

    Detta examensarbete handlar om att implementera Profibus DP i en Atmega644P mikrokontroller, och på så sätt få en Profibus DP-slav som kan användas i utbildningssyfte vid Yrkeshögskolan Novia. Detta examensarbete har inneburit att tillverka ett tilläggskort till det befintliga mikrokontrollerkortet för att kunna koppla in det till en fältbuss som använder sig av RS485-kommunikation. Det har även tillverkats en programkod för Profibus DP-kommunikation till själva mikrokontrollern för att den s...

  18. Mechanism of immunotoxicological effects of tributyltin chloride on murine thymocytes.

    Science.gov (United States)

    Sharma, Neelima; Kumar, Anoop

    2014-04-01

    Tributyltin-chloride, a well-known organotin compound, is a widespread environmental toxicant. The immunotoxic effects of tributyltin-chloride on mammalian system and its mechanism is still unclear. This study is designed to explore the mode of action of tributyltin-induced apoptosis and other parallel apoptotic pathways in murine thymocytes. The earliest response in oxidative stress followed by mitochondrial membrane depolarization and caspase-3 activation has been observed. Pre-treatment with N-acetyl cysteine and buthionine sulfoximine effectively inhibited the tributyltin-induced apoptotic DNA and elevated the sub G1 population, respectively. Caspase inhibitors pretreatment prevent tributyltin-induced apoptosis. Western blot and flow cytometry indicate no translocation of apoptosis-inducing factor and endonuclease G in the nuclear fraction from mitochondria. Intracellular Ca(2+) levels are significantly raised by tributyltin chloride. These results clearly demonstrate caspase-dependent apoptotic pathway and support the role of oxidative stress, mitochondrial membrane depolarization, caspase-3 activation, and calcium during tributyltin-chloride (TBTC)-induced thymic apoptosis.

  19. Post-irradiation thymocyte regeneration after bone marrow transplantation

    International Nuclear Information System (INIS)

    Boersma, W.; Betel, I.; Daculsi, R.; Westen, G. van der

    1981-01-01

    Growth kinetics of the donor-type thymus cell population after transplantation of bone marrow into irradiated syngeneic recipient mice is biphasic. During the first rapid phase of regeneration, lasting until day 19 after transplantation, the rate of development of the donor cells is independent of the number of bone marrow cells inoculated. The second slow phase is observed only when low numbers of bone marrow cells (2.5 x 10 4 ) are transplanted. The decrease in the rate of development is attributed to an efflux of donor cells from the thymus because, at the same time, the first immunologically competent cells are found in spleen. After bone marrow transplantation the regeneration of thymocyte progenitor cells in the marrow is delayed when compared to regeneration of CFUs. Therefore, regenerating marrow has a greatly reduced capacity to restore the thymus cell population. One week after transplantation of 3 x 10 6 cells, 1% of normal capacity of bone marrow is found. It is concluded that the regenerating thymus cells population after bone marrow transplantation is composed of the direct progeny of precursor cells in the inoculum. (author)

  20. HLA-DP and bonemarrow transplantation: DP-incompatibility and severe acute graft versus host disease

    DEFF Research Database (Denmark)

    Ødum, Niels; Platz, P; Jakobsen, B K

    1987-01-01

    The presence of activated T cells as judged from the reaction with monoclonal antibodies (MoAb) against (a) a late stage T cell activation antigen (VLA-1), (b) the interleukin 2 (IL2) receptor (CD25), and (c) four different HLA class II molecules (HLA-DR, DRw52, DQ, and DP) was studied in 15...

  1. Quantitative and Qualitative Changes in V-J α Rearrangements During Mouse Thymocytes Differentiation

    Science.gov (United States)

    Pasqual, Nicolas; Gallagher, Maighréad; Aude-Garcia, Catherine; Loiodice, Mélanie; Thuderoz, Florence; Demongeot, Jacques; Ceredig, Rod; Marche, Patrice Noël; Jouvin-Marche, Evelyne

    2002-01-01

    Knowledge of the complete nucleotide sequence of the mouse TCRAD locus allows an accurate determination V-J rearrangement status. Using multiplex genomic PCR assays and real time PCR analysis, we report a comprehensive and systematic analysis of the V-J recombination of TCR α chain in normal mouse thymocytes during development. These respective qualitative and quantitative approaches give rise to four major points describing the control of gene rearrangements. (a) The V-J recombination pattern is not random during ontogeny and generates a limited TCR α repertoire; (b) V-J rearrangement control is intrinsic to the thymus; (c) each V gene rearranges to a set of contiguous J segments with a gaussian-like frequency; (d) there are more rearrangements involving V genes at the 3′ side than 5′ end of V region. Taken together, this reflects a preferential association of V and J gene segments according to their respective positions in the locus, indicating that accessibility of both V and J regions is coordinately regulated, but in different ways. These results provide a new insight into TCR α repertoire size and suggest a scenario for V usage during differentiation. PMID:12417627

  2. Effect of low dose radiation on thymocyte cytosol and nuclei protein synthesis in mice

    International Nuclear Information System (INIS)

    Meng Qingyong; Chen Shali; Liu Shuzheng

    2003-01-01

    Objective: To the effect of low dose radiation on thymocyte cytosol and nuclei protein synthesis in mice. Methods: The expression of proteins was analyzed by gel filtration with Sephadex G-100 and HPLC based on separation of proteins on thymocyte cytosol and nuclei after whole-body irradiation with 75 mGy X-rays and sham-irradiation, and their biological activity was examined by mouse splenocyte proliferation and chromosome aberration of human peripheral blood lymphocytes. Results: HPLC analysis showed that there was a marked increase in expression of 61.4 kD protein in the extract of thymocyte cytosol and 30.4 kD protein in the extract of thymocyte nuclei in comparison with the corresponding fractions from the sham-irradiated control mice. These protein fractions from the thymocyte cytosol and nuclei of the irradiated mice showed both stimulating effect on normal T cell proliferation and protective effect on chromosome damage induced by high dose radiation. Conclusion: These findings might have implications in study of mechanism of immunoenhancement and cytogenetic adaptive response induced by low dose radiation

  3. Effects of low dose radiation on differentiation, activation and apoptosis of thymocytes in mice

    International Nuclear Information System (INIS)

    Su Xu; Zhang Yingchun; Wan Hong; Liu Shuzheng

    1997-01-01

    Objective: To elucidate the possible mechanism of immunoenhancement after low dose radiation (LDR), the differentiation, activation and apoptosis of thymocytes following low dose irradiation were studied. Method: Kunming mice were whole-body irradiated (WBI) with low dose X-rays. The expressions of CD4, CD8, TCR, CD3, IL-2R, (Ca 2+ ) i , Bcl-2 Bax and apoptosis of thymocytes were analyzed by flow cytometry. Results: It was found that the ratio of T H /T S showed no significant changes after LDR. Thymocyte apoptosis was not increased after LDR. The increase of Bcl-2/Bax ratio after LDR might be one of its mechanisms. LDR could expedites the process of differentiation of, and facilitate the signal transduction in, thymocytes. Conclusion: The results indicate that the mechanism of immunoenhancement might be related to the expedition of the maturation, differentiation and activation of thymocytes, thus up-regulating the capability of supplying more mature T lymphocytes to the periphery by the thymus after LDR

  4. Probing η deformed backgrounds with Dp branes

    Directory of Open Access Journals (Sweden)

    Dibakar Roychowdhury

    2018-03-01

    Full Text Available In this Letter, based on the notion of Gauge/Gravity duality we explore the low frequency behaviour associated with the retarded two point correlators in the ground state of the strongly correlated quantum liquid that is dual to η-deformed background in (2+1D. The massless charge carriers in the dual gauge theory are sourced due to some probe Nf flavour Dp brane configurations in the bulk. In our analysis we stick to the NS sector and compute the two point correlators by turning on fluctuations associated with the worldvolume gauge fields in the bulk spacetime. Our analysis reveals the existence of holographic zero sound modes for (1+1D QFTs those are dual to bosonic η deformed AdS3×S3 with vanishing RR fields.

  5. Activation of chromatin degradation by a protein factor of thymocyte cytoplasm of irradiated mice

    International Nuclear Information System (INIS)

    Soldatenkov, V.A.; Filippovich, I.V.

    1986-01-01

    A cytoplasmic thymocyte fraction isolated 1 h after irradiation of mice accelerates chromatin degradation in isolated nuclei. Treatment of the cytoplasmic fraction by heat and injection of cycloheximide to mice prevent the acceleration of DNA degradation. The analysis of the chromatin degradation products and the kinetics of this process at acid and alkaline pH shows that activation of DNA degradation in thymocytes by a factor obtained from the irradiated cell cytoplasm is specific for a Ca 2+ , Mg 2+ -dependent enzyme. The time- and dose-dependent parameters of the appearance in the thymocyte cytoplasm of the factor influencing degradation of chromatin are in a good agreement with both the time of the onset of its postirradiation degradation and the dose dependence of this process

  6. Prostaglandin D2 effects and DP1 /DP2 receptor distribution in guinea pig urinary bladder out-flow region.

    Science.gov (United States)

    Guan, Na N; Svennersten, Karl; de Verdier, Petra J; Wiklund, N Peter; Gustafsson, Lars E

    2017-02-01

    The proximal urethra and urinary bladder trigone play important roles in continence. We have previously shown that PGD 2 is released from guinea pig bladder urothelium/suburothelium and can inhibit detrusor contractile responses. We presently wished to investigate PGD 2 actions in guinea pig out-flow region and the distribution of DP 1 /DP 2 receptors. The effects of PGD 2 on urothelium-intact trigone and proximal urethra contractility were studied in organ bath experiments. Expression of DP 1 /DP 2 receptor proteins was analysed by western blot. Immunohistochemistry was used to identify distribution of DP 1 /DP 2 receptors. PGD 2 in a dose-dependent manner inhibited trigone contractions induced by electrical field stimulation (EFS) and inhibited spontaneous contractions of the proximal urethra. PGD 2 was equally (trigone) or slightly less potent (urethra) compared with PGE 2 . Expression of DP 1 and DP 2 receptors was found in male guinea pig bladder trigone, neck and proximal urethra. In the trigone and proximal urethra, DP 1 receptors were found on the membrane of smooth muscle cells and weak immunoreactivty was observed in the urothelium. DP 2 receptors were distributed more widespread, weakly and evenly in the urothelium and smooth muscles. Inhibitory effects by PGD 2 on motor activity of guinea pig trigone and proximal urethra are consistent with finding DP 1 and DP 2 receptors located in the urothelium and smooth muscle cells of the trigone and proximal urethra, and PGD 2 may therefore be a modulator of the bladder out-flow region, possibly having a function in regulation of micturition and a role in overactive bladder syndrome. © 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

  7. Strain rate dependent deformation and failure behavior of laser welded DP780 steel joint under dynamic tensile loading

    International Nuclear Information System (INIS)

    Liu, Yang; Dong, Danyang; Wang, Lei; Chu, Xi; Wang, Pengfei; Jin, Mengmeng

    2015-01-01

    Laser welded DP steel joints are used widely in the automotive industry for weight reduction. Understanding the deformation and fracture behavior of the base metal (BM) and its welded joint (WJ), especially at high strain rates, is critical for the design of vehicle structures. This paper is concerned with the effects of strain rate on the tensile properties, deformation and fracture behavior of the laser welded DP780 steel joint. Quasi-static and dynamic tensile tests were performed on the WJ and BM of the DP780 steel using an electromechanical universal testing machine and a high-speed tensile testing machine over a wide range of strain rate (0.0001–1142 s −1 ). The microstructure change and microhardness distribution of the DP780 steel after laser welding were examined. Digital image correlation (DIC) and high-speed photography were employed for the strain measurement of the DP780 WJ during dynamic tensile tests. The DP780 WJ is a heterogeneous structure with hardening in fusion zone (FZ) and inner heat-affected zone (HAZ), and softening in outer HAZ. The DP780 BM and WJ exhibit positive strain rate dependence on the YS and UTS, which is smaller at lower strain rates and becomes larger with increasing strain rate, while ductility in terms of total elongation (TE) tends to increase under dynamic loading. Laser welding leads to an overall reduction in the ductility of the DP780 steel. However, the WJ exhibits a similar changing trend of the ductility to that of the BM with respect to the strain rate over the whole strain rate range. As for the DP780 WJ, the distance of tensile failure location from the weld centerline decreases with increasing strain rate. The typical ductile failure characteristics of the DP780 BM and WJ do not change with increasing strain rate. DIC measurements reveal that the strain localization starts even before the maximum load is attained in the DP780 WJ and gradual transition from uniform strains to severely localized strains occurs

  8. Strain rate dependent deformation and failure behavior of laser welded DP780 steel joint under dynamic tensile loading

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Yang, E-mail: liuyang@mail.neu.edu.cn [Key Laboratory for Anisotropy and Texture of Materials, Ministry of Education, Northeastern University, Shenyang 110819 (China); Dong, Danyang, E-mail: dongdanyang@mail.neu.edu.cn [College of Science, Northeastern University, Shenyang 110819 (China); Wang, Lei, E-mail: wanglei@mail.neu.edu.cn [Key Laboratory for Anisotropy and Texture of Materials, Ministry of Education, Northeastern University, Shenyang 110819 (China); Chu, Xi, E-mail: chuxi.ok@163.com [College of Science, Northeastern University, Shenyang 110819 (China); Wang, Pengfei, E-mail: wpf1963871400@163.com [College of Science, Northeastern University, Shenyang 110819 (China); Jin, Mengmeng, E-mail: 24401878@163.com [College of Science, Northeastern University, Shenyang 110819 (China)

    2015-03-11

    Laser welded DP steel joints are used widely in the automotive industry for weight reduction. Understanding the deformation and fracture behavior of the base metal (BM) and its welded joint (WJ), especially at high strain rates, is critical for the design of vehicle structures. This paper is concerned with the effects of strain rate on the tensile properties, deformation and fracture behavior of the laser welded DP780 steel joint. Quasi-static and dynamic tensile tests were performed on the WJ and BM of the DP780 steel using an electromechanical universal testing machine and a high-speed tensile testing machine over a wide range of strain rate (0.0001–1142 s{sup −1}). The microstructure change and microhardness distribution of the DP780 steel after laser welding were examined. Digital image correlation (DIC) and high-speed photography were employed for the strain measurement of the DP780 WJ during dynamic tensile tests. The DP780 WJ is a heterogeneous structure with hardening in fusion zone (FZ) and inner heat-affected zone (HAZ), and softening in outer HAZ. The DP780 BM and WJ exhibit positive strain rate dependence on the YS and UTS, which is smaller at lower strain rates and becomes larger with increasing strain rate, while ductility in terms of total elongation (TE) tends to increase under dynamic loading. Laser welding leads to an overall reduction in the ductility of the DP780 steel. However, the WJ exhibits a similar changing trend of the ductility to that of the BM with respect to the strain rate over the whole strain rate range. As for the DP780 WJ, the distance of tensile failure location from the weld centerline decreases with increasing strain rate. The typical ductile failure characteristics of the DP780 BM and WJ do not change with increasing strain rate. DIC measurements reveal that the strain localization starts even before the maximum load is attained in the DP780 WJ and gradual transition from uniform strains to severely localized strains

  9. Possible nature and specificity of a protein factor favoringsolubilization of chromatin from irradiated animal thymocytes

    International Nuclear Information System (INIS)

    Soldatenkov, V.A.; Trebenok, Z.A.; Filippovich, I.V.

    1989-01-01

    It is shown that activation of endonucleolysis of thymocyte nuclear chromatin by protein factor from the cells of irradiated animals is not conditioned by its nuclease activity or ability to activate Ca 2+ , Mg 2+ - dependent lymphocyte endonuclease. DNA degradation character and kinetics of accumulation of the forming products doesn't change in autolysis of thymocyte nucleus. It is assumed that protein factor doesn't participate in starting mechanisms of postirradiation chromatin degradation but can be of significance at delayed stages of the process. The discovered effect is characterized by tissue and specific characteristic

  10. SCL, LMO1 and Notch1 Reprogram Thymocytes into Self-Renewing Cells

    Science.gov (United States)

    Rojas-Sutterlin, Shanti; Herblot, Sabine; Hébert, Josée; Sauvageau, Guy; Lemieux, Sébastien; Lécuyer, Eric; Veiga, Diogo F. T.; Hoang, Trang

    2014-01-01

    The molecular determinants that render specific populations of normal cells susceptible to oncogenic reprogramming into self-renewing cancer stem cells are poorly understood. Here, we exploit T-cell acute lymphoblastic leukemia (T-ALL) as a model to define the critical initiating events in this disease. First, thymocytes that are reprogrammed by the SCL and LMO1 oncogenic transcription factors into self-renewing pre-leukemic stem cells (pre-LSCs) remain non-malignant, as evidenced by their capacities to generate functional T cells. Second, we provide strong genetic evidence that SCL directly interacts with LMO1 to activate the transcription of a self-renewal program coordinated by LYL1. Moreover, LYL1 can substitute for SCL to reprogram thymocytes in concert with LMO1. In contrast, inhibition of E2A was not sufficient to substitute for SCL, indicating that thymocyte reprogramming requires transcription activation by SCL-LMO1. Third, only a specific subset of normal thymic cells, known as DN3 thymocytes, is susceptible to reprogramming. This is because physiological NOTCH1 signals are highest in DN3 cells compared to other thymocyte subsets. Consistent with this, overexpression of a ligand-independent hyperactive NOTCH1 allele in all immature thymocytes is sufficient to sensitize them to SCL-LMO1, thereby increasing the pool of self-renewing cells. Surprisingly, hyperactive NOTCH1 cannot reprogram thymocytes on its own, despite the fact that NOTCH1 is activated by gain of function mutations in more than 55% of T-ALL cases. Rather, elevating NOTCH1 triggers a parallel pathway involving Hes1 and Myc that dramatically enhances the activity of SCL-LMO1 We conclude that the acquisition of self-renewal and the genesis of pre-LSCs from thymocytes with a finite lifespan represent a critical first event in T-ALL. Finally, LYL1 and LMO1 or LMO2 are co-expressed in most human T-ALL samples, except the cortical T subtype. We therefore anticipate that the self-renewal network

  11. HLA-DP antigens in patients with alopecia areata

    DEFF Research Database (Denmark)

    Ødum, Niels; Morling, N; Georgsen, J

    1990-01-01

    The distribution of HLA-DP antigens were studied in 41 patients with alopecia areata (AA) and 188 ethnically matched controls. An increase of DR4 and possibly DR5 in 24 of these patients has previously been reported. HLA-DP typing for DPw1 through w6 and the local specificity, CDP HEI, was perfor...

  12. Experimental demonstration of multi-pilot aided carrier phase estimation for DP-64QAM and DP-256QAM

    NARCIS (Netherlands)

    Pajovic, M.; Millar, D.S.; Koike-Akino, T.; Maher, R.; Lavery, D.; Alvarado, A.; Paskov, M.; Kojima, K.; Parsons, K.; Thomsen, B.C.; Savory, S.J.; Bayvel, P.

    2015-01-01

    We present a statistical inference based multi-pilot aided CPE algorithm and analyze its performance via simulations. We experimentally verify LDPC coded back-to-back performance using 10 GBd DP-64QAM and DP-256QAM modulation, with transmitter and receiver linewidths of 100 kHz.

  13. HLA-DP and bone marrow transplantation: DP-incompatibility and severe acute graft versus host disease

    DEFF Research Database (Denmark)

    Ødum, Niels; Platz, P; Jakobsen, B K

    1987-01-01

    Thirteen recipients of HLA-haploidentical, DR compatible bone marrow (BM) and the corresponding BM donors were HLA-DP typed using primed lymphocyte typing (PLT). Severe acute GVHD (greater than or equal to grade 2) developed within 3 months after BM-transplantation in all of eight recipients of DP...... a role as transplantation antigens....

  14. CD4+/CD8+ double-positive T cells

    DEFF Research Database (Denmark)

    Overgaard, Nana H; Jung, Ji-Won; Steptoe, Raymond J

    2015-01-01

    CD4(+)/CD8(+) DP thymocytes are a well-described T cell developmental stage within the thymus. However, once differentiated, the CD4(+) lineage or the CD8(+) lineage is generally considered to be fixed. Nevertheless, mature CD4(+)/CD8(+) DP T cells have been described in the blood and peripheral...... cells, CD4(+)/CD8(+) T cell populations, outside of the thymus, have recently been described to express concurrently ThPOK and Runx3. Considerable heterogeneity exists within the CD4(+)/CD8(+) DP T cell pool, and the function of CD4(+)/CD8(+) T cell populations remains controversial, with conflicting...... reports describing cytotoxic or suppressive roles for these cells. In this review, we describe how transcriptional regulation, lineage of origin, heterogeneity of CD4 and CD8 expression, age, species, and specific disease settings influence the functionality of this rarely studied T cell population....

  15. The involvement of nuclear nucleases in rat thymocyte DNA degradation after γ-irradiation

    International Nuclear Information System (INIS)

    Nikonova, L.V.; Nelipovich, P.A.; Umansky, S.R.

    1982-01-01

    Possible mechanisms of internucleosomal DNA fragmentation in thymocytes of irradiated rats were studied. It was shown that thymocyte nuclei contain at least two nucleases that cleave DNA between nucleosomes - a Ca 2+ /Mg 2+ -dependent nuclease and an acidic one which does not depend on bivalent ions. 2 and 3 h after irradiation at a dose of 10 Gy the initial rate of DNA cleavage by Ca 2+ /Mg 2+ -dependent nuclease in isolated nuclei increased three and seven times, respectively, but the kinetics of DNA digestion by acidic nuclease did not change. The experiments with cycloheximide indicated that Ca 2+ /Mg 2+ -dependent endonuclease turns over at a high rate. The activity of the cytoplasmic acidic and Mg 2+ -dependent nucleases was shown to increase (by 40 and 50%, respectively) 3h after irradiation. The effect is caused by the de novo synthesis of the nucleases. At the same time the activity of nuclear nucleases did not essentially change. The chromatin isolated from rat thymocytes 3 h after irradiation did not differ in its sensitivity to some exogenic nucleases (DNAase I, micrococcal nuclease and nuclease from Serratia marcescens) from the control. Thus, Ca 2+ /Mg 2+ -dependent endonuclease seems to be responsible for the postirradiation internucleosomal DNA fragmentation in dying thymocytes. (Auth.)

  16. Radiation-induced apoptosis in thymocytes as determined by flow cytometry

    International Nuclear Information System (INIS)

    Su Xu; Zhang Yingchun; Liu Shuzheng

    1995-01-01

    Programmed cell death (PCD), or apoptosis, is a conceptually different way of cell death from necrosis. PCD plays an important role in immunologic regulation, PCD in thymocytes was analyzed by flow cytometry following in vitro X-irradiation. It was found that culturing of thymocytes could induce PCD which showed a time dependent increase. Four hours after culturing, 16% of thymocytes was found in the Ao region (PCD is shown in the Ao region of the histogram of flow cytometry). PCD in thymocytes showed a time dependent increase after 2.0 Gy X-irradiation, being significantly higher than that in the control at the same culturing time. 24 hours after X-irradiation in vitro, it was found that with doses below 100 mGy PCD was not significantly different from the control at the same culturing time. But when the doses were above 100 mGy, PCD showed a dose dependent increase, being significantly higher than that of the control at the same culturing time. These results are important in the understanding of the biological effects of low dose radiation

  17. Protective effects of melatonin on damage of thymocytes in mice induced by ionizing radiation

    International Nuclear Information System (INIS)

    Zhang Xuan; Wang Zhenqi; Liu Yang; Gong Shouliang; Zhang Ming; Liu Shuzheng

    2004-01-01

    Objective: To explore the effects of melatonin (MLT) on the damage of mouse thymocytes in vivo induced by ionizing radiation and its mechanism. Methods: The exogenous MLT was given to Kunming mice to establish the animal models of single and successive administration of MLT through intraperitoneal injection before whole-body irradiation with 1 Gy X-rays. For single administration of MLT, the apoptotic body percentage (ABP) and DNA lytic rate (DLR) in the thymocytes were determined with flow cytometry and fluorospectrophotometry, respectively, 12 h after irradiation. For successive administration of MLT, 3 H-TdR incorporative rate (HTIR ) was determined 24 h after irradiation. Results: The number of thymocytes in single administration group was significantly lower than that in the sham-irradiation group 12 h after irradiation with 1 Gy X-rays (P -1 MLT group was significantly higher, while the ABP and DLR were significantly lower than those in 0 mg·kg -1 MLT group (simple irradiation, P -1 MLT were significantly higher than that in 0 mg·kg -1 MLT group (P -1 MLT group was also significantly higher (P<0.05). Conclusion: The administration of exogenous MLT before irradiation can decrease the damage of mouse thymocytes induced by ionizing radiation, and has the protective effect on immune functions in mice. (authors)

  18. Human natural killer cell committed thymocytes and their relation to the T cell lineage

    NARCIS (Netherlands)

    Sánchez, M. J.; Spits, H.; Lanier, L. L.; Phillips, J. H.

    1993-01-01

    Recent studies have demonstrated that mature natural killer (NK) cells can be grown from human triple negative (TN; CD3-, CD4-, CD8-) thymocytes, suggesting that a common NK/T cell precursor exists within the thymus that can give rise to both NK cells and T cells under appropriate conditions. In the

  19. Cytotoxicity of Environmentally Relevant Concentrations of Aluminum in Murine Thymocytes and Lymphocytes

    Directory of Open Access Journals (Sweden)

    Jamal Kamalov

    2011-01-01

    Full Text Available The effects of low concentrations of aluminum chloride on thymocytes and lymphocytes acutely dissociated from young mice were studied using flow cytometry with a DNA-binding dye. We demonstrate a rapid and dose-dependent injury in murine thymocytes and lymphocytes resulting from exposure to aluminum, as indicated by an increase in the entry into the cell of the DNA-binding dye, propidium iodine. A 60-minute exposure to 10 μM AlCl3 caused damage of about 5% of thymocytes, while 50% were injured after 10 minutes at 20 μM. Nearly all thymocytes showed evidence of damage at 30 μM AlCl3 after only 5 minutes of incubation. In lymphocytes, injury was observed at 15 μM AlCl3 and less than 50% of cells were injured after a 60-minute exposure to 20 μM. Injury only rarely proceeded to rapid cell death and was associated with cell swelling. These results suggest that aluminum has cytotoxic effects on cells of the immune system.

  20. Microstructure and dynamic tensile behavior of DP600 dual phase steel joint by laser welding

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Danyang, E-mail: dongdanyang@mail.neu.edu.cn [College of Science, Northeastern University, No. 11, Lane 3, WenHua Road, HePing District, Shenyang 110819 (China); Liu, Yang, E-mail: liuyang@mail.neu.edu.cn [Key Laboratory for Anisotropy and Texture of Materials, Ministry of Education, Northeastern University, Shenyang 110819 (China); Yang, Yuling, E-mail: yulingyang@mail.neu.edu.cn [College of Science, Northeastern University, No. 11, Lane 3, WenHua Road, HePing District, Shenyang 110819 (China); Li, Jinfeng, E-mail: lijinfengboda@163.com [College of Science, Northeastern University, No. 11, Lane 3, WenHua Road, HePing District, Shenyang 110819 (China); Ma, Min, E-mail: sharon6789@163.com [College of Science, Northeastern University, No. 11, Lane 3, WenHua Road, HePing District, Shenyang 110819 (China); Jiang, Tao, E-mail: tao.jiang906@yahoo.com [College of Science, Northeastern University, No. 11, Lane 3, WenHua Road, HePing District, Shenyang 110819 (China)

    2014-01-31

    Dual phase (DP) steels have been widely used in the automotive industry to reduce vehicle weight and improve car safety. In such applications welding and joining have to be involved, which would lead to a localized change of the microstructure and property, and create potential safety and reliable issues under dynamic loading. The aim of the present study is to examine the rate-dependent mechanical properties, deformation and fracture behavior of DP600 steel and its welded joint (WJ) produced by Nd:YAG laser welding over a wide range of strain rates (0.001–1133 s{sup −1}). Laser welding results in not only significant microhardness increase in the fusion zone (FZ) and inner heat-affected zone (HAZ), but also the formation of a softened zone in the outer HAZ. The yield strength (YS) of the DP600 steel increases and the ultimate tensile strength (UTS) remains almost unchanged, but the ductility decreases after welding. The DP600 base metal (BM) and WJ are of positive strain rate sensitivity and show similar stress–strain response at all studied strain rates. The enhanced ductility at strain rates ranging from 1 to 100 s{sup −1} is attributed to the retardation of the propagation of plastic strain localization due to the positive strain rate sensitivity and the thermal softening caused by deformation induced adiabatic temperature rise during dynamic tensile deformation. The tensile failure occurs in the inner HAZ of the joint and the distance of failure location from the weld centerline decreases with increasing strain rate. The mechanism for the changing failure location can be related to the different strain rate dependence of the plastic deformation behavior of the microstructures in various regions across the joint. The DP600 WJ absorbs more energy over the whole measured strain rates than that of the BM due to the higher strength at the same strain when the deformation only up to 10% is considered.

  1. Inhibition of erythroid cell growth by allogeneic murine lymphocytes. Evidence for a synergism between lymph node cells and thymocytes

    International Nuclear Information System (INIS)

    Blomgren, H.; Jacobsson, H.

    1974-01-01

    Murine lymphoid cells from thymus and lymph nodes were tested for synergistic response in a graft-vs-host test. The test is based on the principle that allogeneic lymphocytes inhibit erythroid cell proliferation in the spleens of irradiated mice infused with syngeneic bone marrow cells. Mixtures of thymocytes and lymph node cells from the same parental strain yielded graft-vs-host responses in irradiated F 1 -hybrids higher than expected by summing the responses of the two cell populations tested separately. A similar synergistic response was obtained using mixtures of thymocytes and lymph node cells obtained from the two parental strains of the hybrid, whereas such an effect was not detected using mixtures of lymph node cells or mixtures of thymocytes from the two parental strains. Nor could synergy be demonstrated between parental strain lymph node cells and thymocytes syngeneic with the bone marrow target cells. Thymocytes obtained from one parental strain which was injected into its irradiated F 1 -hybrid transformed into a population of sensitized cells in the spleens of the recipients. This transformation was suppressed by the simultaneous injection of lymph node cells from the second parental strain. Since there is a synergistic immune response by such cell mixtures it is concluded that thymocytes may enhance the graft-vs-host response of lymph node cells. Parental strain thymocytes and lymph node cells, the latter being specifically immunologically tolerant to the bone marrow target cells, failed to give a synergistic response indicating that thymocytes do not transform unresponsive lymphocytes into responsive, but rather enhance the reactivity of existing, specifically responsive cells. The results thus show that thymocytes may enhance the response of lymph node cells in this specific graft-vs-host assay

  2. Tissue breathing and topology of rats thymocytes surface under acute total γ-irradiation.

    Science.gov (United States)

    Nikitina, I A; Gritsuk, A I

    2017-12-01

    Assessment of the effect of single total γ irradiation to the parameters of mitochondrial oxidation and the topology of the thymocyte surface. The study was performed in sexually mature white outbreeding male rats divided into three groups: two experimental and one control. The states of energy metabolism were determined by the rate of oxygen consumption by the thymus tissues on endogenous substrates at the presence of 2,4 dinitrophenol, uncoupler of a tissue breathing (TB) and oxidative phosphorylation (OP) after a single total γ irradiation at a dose of 1.0 Gy at 3, 10, 40 and 60 days. The topology of thymus cells was assessed using atomic force microscopy (AFM) and scanning electron microscopy (SEM). On the 3rd and 10th days after total gamma irradiation at a dose of 1.0 Gy, a significant decrease in respira tory activity was determined in thymus tissues on endogenous substrates. Simultaneously, on the 3rd day, pro nounced changes in the morphological parameters of thymocytes (height, volume, area of contact with the sub strate) and the topology of their surface were also observed. On the 10th day after irradiation, most of the morpho logical parameters of thymocytes, except for their volume, were characterized by restoration to normal. In the long term (on the 30th and 60th days after exposure), a gradual but not complete recovery of the respiratory activity of thymocytes was observed, accompanied by an increase in the degree of dissociation of TD and OP. The obtained data reflect and refine mechanisms of post radiation repair of lymphopoiesis, showing the presence of conjugated changes in the parameters of aerobic energy metabolism of thymocytes, morphology and topology of their surface. The synchronism of changes in the parameters under study is a reflection of the state of the cytoskeleton, the functional activity of which largely depends on the level and efficiency of mitochondrial oxidation. І. A. Nikitina, A. I. Gritsuk.

  3. Dose-effect relationship of apoptosis induced by fission-neutron in murine thymocytes

    International Nuclear Information System (INIS)

    Yuan Bin; Li Liang; Xue Wencheng; Sun Jianmin; Wang Baoqin

    2000-01-01

    Objective: To investigate the effectiveness of high LET fission-neutron to induce apoptosis in murine thymocytes and to compare it with that of low LET 60 Co γ-ray. Methods: Apoptosis induction was studied qualitatively by light and transmission electron microscopy and DNA gel electrophoresis,also quantitatively by flow cytometry(FCM) and diphenylamine (DPA)methods. Results: DNA ladders of murine thymocytes were detectable, the typical apoptosis of thymocytes could be observed morphologically by means of light and electron microscopy at 6 h after fission-neutron irradiation with doses ranging from 0.5 to 5.0 Gy, meanwhile the percentages of apoptosis increased with increasing doses. After exposure to γ-rays with doses ranging from 1.0 to 30 Gy, the experimental results were similar to those from neutron radiation. The incidence of apoptosis peaked at about 20 Gy, the percentages did not increase further when doses increased. Conclusion: Apoptosis of murine thymocytes can be induced when mice are exposed to either fission-neutron (0.5-5.0 Gy) or to γ-ray (1-30 Gy). Although the relationship between apoptosis and radiation doses is similar, the percentage of apoptosis induced by neutron irradiation is higher than that induced by γ-irradiation. The RBE values of fission-neutron for inducing apoptosis murine thymocytes are 2.09 (by FCM method) and 2.37 (by DPA method), respectively. These results also suggest that fission-neutron-induced murine immune tissue is more severe than that induced by γ-rays at several hours post-irradiation and this might be the basis for heavy damage to immune tissues induced by fission-neutron-irradiation in later period

  4. Somatodendritic and excitatory postsynaptic distribution of neuron-type dystrophin isoform, Dp40, in hippocampal neurons

    International Nuclear Information System (INIS)

    Fujimoto, Takahiro; Itoh, Kyoko; Yaoi, Takeshi; Fushiki, Shinji

    2014-01-01

    Highlights: • Identification of dystrophin (Dp) shortest isoform, Dp40, is a neuron-type Dp. • Dp40 expression is temporally and differentially regulated in comparison to Dp71. • Somatodendritic and nuclear localization of Dp40. • Dp40 is localized to excitatory postsynapses. • Dp40 might play roles in dendritic and synaptic functions. - Abstract: The Duchenne muscular dystrophy (DMD) gene produces multiple dystrophin (Dp) products due to the presence of several promoters. We previously reported the existence of a novel short isoform of Dp, Dp40, in adult mouse brain. However, the exact biochemical expression profile and cytological distribution of the Dp40 protein remain unknown. In this study, we generated a polyclonal antibody against the NH 2 -terminal region of the Dp40 and identified the expression profile of Dp40 in the mouse brain. Through an analysis using embryonic and postnatal mouse cerebrums, we found that Dp40 emerged from the early neonatal stages until adulthood, whereas Dp71, an another Dp short isoform, was highly detected in both prenatal and postnatal cerebrums. Intriguingly, relative expressions of Dp40 and Dp71 were prominent in cultured dissociated neurons and non-neuronal cells derived from mouse hippocampus, respectively. Furthermore, the immunocytological distribution of Dp40 was analyzed in dissociated cultured neurons, revealing that Dp40 is detected in the soma and its dendrites, but not in the axon. It is worthy to note that Dp40 is localized along the subplasmalemmal region of the dendritic shafts, as well as at excitatory postsynaptic sites. Thus, Dp40 was identified as a neuron-type Dp possibly involving dendritic and synaptic functions

  5. Somatodendritic and excitatory postsynaptic distribution of neuron-type dystrophin isoform, Dp40, in hippocampal neurons

    Energy Technology Data Exchange (ETDEWEB)

    Fujimoto, Takahiro; Itoh, Kyoko, E-mail: kxi14@koto.kpu-m.ac.jp; Yaoi, Takeshi; Fushiki, Shinji

    2014-09-12

    Highlights: • Identification of dystrophin (Dp) shortest isoform, Dp40, is a neuron-type Dp. • Dp40 expression is temporally and differentially regulated in comparison to Dp71. • Somatodendritic and nuclear localization of Dp40. • Dp40 is localized to excitatory postsynapses. • Dp40 might play roles in dendritic and synaptic functions. - Abstract: The Duchenne muscular dystrophy (DMD) gene produces multiple dystrophin (Dp) products due to the presence of several promoters. We previously reported the existence of a novel short isoform of Dp, Dp40, in adult mouse brain. However, the exact biochemical expression profile and cytological distribution of the Dp40 protein remain unknown. In this study, we generated a polyclonal antibody against the NH{sub 2}-terminal region of the Dp40 and identified the expression profile of Dp40 in the mouse brain. Through an analysis using embryonic and postnatal mouse cerebrums, we found that Dp40 emerged from the early neonatal stages until adulthood, whereas Dp71, an another Dp short isoform, was highly detected in both prenatal and postnatal cerebrums. Intriguingly, relative expressions of Dp40 and Dp71 were prominent in cultured dissociated neurons and non-neuronal cells derived from mouse hippocampus, respectively. Furthermore, the immunocytological distribution of Dp40 was analyzed in dissociated cultured neurons, revealing that Dp40 is detected in the soma and its dendrites, but not in the axon. It is worthy to note that Dp40 is localized along the subplasmalemmal region of the dendritic shafts, as well as at excitatory postsynaptic sites. Thus, Dp40 was identified as a neuron-type Dp possibly involving dendritic and synaptic functions.

  6. Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.

    Science.gov (United States)

    Houddane, Amina; Bultot, Laurent; Novellasdemunt, Laura; Johanns, Manuel; Gueuning, Marie-Agnès; Vertommen, Didier; Coulie, Pierre G; Bartrons, Ramon; Hue, Louis; Rider, Mark H

    2017-06-01

    Proliferating cells depend on glycolysis mainly to supply precursors for macromolecular synthesis. Fructose 2,6-bisphosphate (Fru-2,6-P 2 ) is the most potent positive allosteric effector of 6-phosphofructo-1-kinase (PFK-1), and hence of glycolysis. Mitogen stimulation of rat thymocytes with concanavalin A (ConA) led to time-dependent increases in lactate accumulation (6-fold), Fru-2,6-P 2 content (4-fold), 6-phosphofructo-2-kinase (PFK-2)/fructose-2,6-bisphosphatase isoenzyme 3 and 4 (PFKFB3 and PFKFB4) protein levels (~2-fold and ~15-fold, respectively) and rates of cell proliferation (~40-fold) and protein synthesis (10-fold) after 68h of incubation compared with resting cells. After 54h of ConA stimulation, PFKFB3 mRNA levels were 45-fold higher than those of PFKFB4 mRNA. Although PFKFB3 could be phosphorylated at Ser461 by protein kinase B (PKB) in vitro leading to PFK-2 activation, PFKFB3 Ser461 phosphorylation was barely detectable in resting cells and only increased slightly in ConA-stimulated cells. On the other hand, PFKFB3 and PFKFB4 mRNA levels were decreased (90% and 70%, respectively) by exposure of ConA-stimulated cells to low doses of PKB inhibitor (MK-2206), suggesting control of expression of the two PFKFB isoenzymes by PKB. Incubation of thymocytes with ConA resulted in increased expression and phosphorylation of the translation factors eukaryotic initiation factor-4E-binding protein-1 (4E-BP1) and ribosomal protein S6 (rpS6). Treatment of ConA-stimulated thymocytes with PFK-2 inhibitor (3PO) or MK-2206 led to significant decreases in Fru-2,6-P 2 content, medium lactate accumulation and rates of cell proliferation and protein synthesis. These data were confirmed by using siRNA knockdown of PFKFB3, PFKFB4 and PKB α/β in the more easily transfectable Jurkat E6-1 cell line. The findings suggest that increased PFKFB3 and PFKFB4 expression, but not increased PFKFB3 Ser461 phosphorylation, plays a role in increasing glycolysis in mitogen

  7. Left-right entanglement entropy of Dp-branes

    Energy Technology Data Exchange (ETDEWEB)

    Zayas, Leopoldo A. Pando [The Abdus Salam International Centre for Theoretical Physics,Strada Costiera 11, 34014 Trieste (Italy); Michigan Center for Theoretical Physics, Randall Laboratory of Physics,The University of Michigan,450 Church Street, Ann Arbor, MI 48109-1120 (United States); Quiroz, Norma [Departamento de Ciencias Exactas, Tecnología y Metodología,Centro Universitario del Sur, Universidad de Guadalajara,Enrique Arreola Silva 883, C.P. 49000, Cd. Guzmán, Jalisco (Mexico)

    2016-11-04

    We compute the left-right entanglement entropy for Dp-branes in string theory. We employ the CFT approach to string theory Dp-branes, in particular, its presentation as coherent states of the closed string sector. The entanglement entropy is computed as the von Neumann entropy for a density matrix resulting from integration over the left-moving degrees of freedom. We discuss various crucial ambiguities related to sums over spin structures and argue that different choices capture different physics; however, we advance a themodynamic argument that seems to favor a particular choice of replica. We also consider Dp branes on compact dimensions and verify that the effects of T-duality act covariantly on the Dp brane entanglement entropy. We find that generically the left-right entanglement entropy provides a suitable generalization of boundary entropy and of the D-brane tension.

  8. PROFIBUS-DP AĞ TABANLI BİNA OTOMASYONU TASARIMI

    OpenAIRE

    YILMAZ, Cemal; ÜNCÜ, İ. Serkan

    2006-01-01

    In this study, a building automation has been designed by using the Profibus DP (Process Field Bus- Decentralized Periphery) network. In the study; fire alarm, thief alarm, lighting, power, humidity and temperature control have been implemented. The data from building has been transmitted to the Profibus-DP network via control point located on the flats. The data taken from the building has been collected in the main control unit to achieve overall control of the system. The work has provided...

  9. Changes in DP systems to match order processing in pipeline engineering and manufacturing

    International Nuclear Information System (INIS)

    Pletschen, W.; Weber, J.

    1987-01-01

    Pipelines hold a pivotal position as the linking element between the mechanical and the electrical engineering components; hence, their production and machining is highly important. Information systems like GRAPLAN, MISTER, PVK, DOPLAS, and PFPD have been used successfully in recent years and are being constantly upgraded to meet the requirements on advanced nuclear pipeline systems which call for DP systems featuring variable dimensioning and suitable interlinkage capacities. (DG) [de

  10. Growth of single T cells and single thymocytes in a high cloning efficiency filler-cell free microculture system.

    Science.gov (United States)

    Chen, W F; Ewing, T; Scollay, R; Shortman, K

    1988-01-01

    A high cloning-efficiency microculture system is described in which single T cells, stimulated to divide by phorbol ester and calcium ionophore, grow rapidly under the influence of purified growth factors in the absence of other cells. The kinetics of clonal growth has been monitored over a five day period by phase-contrast microscopy. Mature peripheral T cells, and mature subpopulations from the thymus, responded with a cloning efficiency over 80%; they required IL-2 as a minimum but several other factors enhanced growth. Ly2+L3T4- thymocytes (mean doubling time 10.4 hr) grew more rapidly than Ly2-L3T4+ thymocytes (mean doubling time 15.2 hr). Early (Ly2-L3T4-) thymocytes responded with a cloning efficiency of 60%; their efficient growth was dependent on both IL-1 and IL-2. The typical Ly2+L3T4+ cortical thymocyte did not grow under these conditions.

  11. Early dominance of irradiated host cells in the responder profiles of thymocytes from P → F1 radiation chimeras

    International Nuclear Information System (INIS)

    Korngold, R.; Bennink, J.R.; Doherty, P.C.

    1981-01-01

    The number of cells in the thymus of radiation (1000 rad) chimeras increases approximately 10-fold between 7 and 14 days after reconstitution with bone marrow. At least 50% of the cells in thymus on day 14 are of host origin and respond to virus presented in the context of both H-2/sup k/ and H-2/sup b/ when primed in irradiated, virus-infected (b x k)F 1 recipients. Strong CTL responses can be generated from thymocytes of donor origin on day 21. All evidence of a significant host thymocyte component has disappeared by day 28. The responsiveness of 14-day thymocytes is not abrogated by pretreatment of the mice used to make the chimeras with anti-thymocyte serum or by using doses of irradiation as high as 1200 rads to eliminate host components

  12. Role of adrenal hormones and prostaglandins in the control of mouse thymocytes lysis.

    Science.gov (United States)

    Durant, S; Seillan, C; Duval, D; Homo-Delarche, F

    1984-01-01

    The cytolytic actions of glucocorticoids and of agents increasing cyclic AMP were studied in vitro in thymocyte suspensions isolated from adrenalectomized or hydrocortisone-treated mice. Although considered as corticoresistant cells, the thymocytes isolated from hydrocortisone-treated mice were lysed to the same extent although more slowly in vitro by dexamethasone than whole thymocyte populations (i.e. corticosensitive cells). Moreover, these two cell populations were shown to contain comparable amounts of glucocorticoid receptors and to be almost equally sensitive to the metabolic effects of glucocorticoids when measured by inhibition of RNA and DNA synthesis. Studies performed with corticosensitive cells showed that prostaglandin E2, isoproterenol and dibutyrilcyclic AMP were also able to induce cell lysis and that, isoproterenol and dexamethasone exerted additive cytolytic action in vitro. In vivo experiments showed also an additive effect of steroids and isoproterenol on thymus atrophy. In contrast, cells isolated from hydrocortisone-treated animals were not sensitive to the cytotoxic action of prostaglandin E2, isoproterenol and dibutyril cyclic AMP. This difference between the two populations was not associated with any difference in the responsiveness of adenylate cyclase as determined following isoproterenol-induced accumulation of cyclic AMP. The cytolytic action of dexamethasone but also that of prostaglandin E2 and isoproterenol, could be blocked in the presence of cycloheximide, an inhibitor of protein synthesis, thus suggesting that glucocorticoids and agents increasing cyclic AMP control the synthesis of some proteins involved in the triggering of cell lysis. Among the hypotheses proposed to explain the differences between in vitro and in vivo sensitivity of lymphoid cell to glucocorticoids, it was suggested that the drug may in vivo indirectly control the viability or the proliferation of thymocytes through the release of other mediators. We have

  13. Structural chromatin organization as a factor determining the rate of chromatin endonucleolysis in irradiated and intact thymocytes

    International Nuclear Information System (INIS)

    Ryabchenko, N.I.; Ivannik, B.P.

    1987-01-01

    A study was made of chromatin endonucleolysis in hypotonized thymocytes incubating in digestive buffers containing different concentrations of potassium, magnesium, calcium, and mercaptoethanol. Inhibition of endonucleolysis by univalent cation during the first 20 min of incubation was followed by intensive chromatin degradation. A decrease in free potassium content retarded chromatin degradation and enhanced the inhibiting effect of the univalent cations. The regularities of changes in the rate of chromatin endonucleolysis in different digestive buffers were similar with both exposed and intact thymocytes

  14. Anti-thymocyte serum as part of an immunosuppressive regimen in treating haematological immune-mediated diseases in dogs.

    Science.gov (United States)

    Cuq, B; Blois, S L; Mathews, K A

    2017-06-01

    To report the outcomes associated with the use of rabbit anti-dog thymocyte serum in dogs with haematological immune-mediated diseases. Medical records from 2000 to 2016 of patients diagnosed with immune-mediated haemolytic anaemia, immune-mediated thrombocytopenia, pancytopenia and myelofibrosis were reviewed. All dogs had a severe or refractory disease and received rabbit anti-dog thymocyte serum. Lymphocyte counts were used to monitor the immediate anti-thymocyte effect of therapy; long-term patient outcome was recorded. A total of 10 dogs were included. All dogs except one had a notable decrease in their lymphocyte count after rabbit anti-dog thymocyte serum; four of nine had a decrease to less than 10% of the initial lymphocyte count and one dog reached 10·8%. All dogs were discharged from the hospital following their treatment. The dog with no alteration of lymphocyte count following therapy with rabbit anti-dog thymocyte serum had refractory immune mediated haemolytic anemia and was euthanised within two weeks. All other cases achieved clinical remission with immunosuppressive therapy eventually being tapered (3 of 10) or discontinued (6 of 10). Rabbit anti-dog thymocyte serum therapy might be of interest as an adjunctive therapy in refractory immune-mediated diseases and suppressed lymphocyte counts in most dogs. © 2017 British Small Animal Veterinary Association.

  15. Sequential appearance of thymocyte subpopulations and T cell antigen receptor gene messages in the mouse thymus after sublethal irradiation

    International Nuclear Information System (INIS)

    Tomooka, S.; Matsuzaki, G.; Kishihara, K.; Tanaka, K.; Yoshikai, Y.; Taniguchi, K.; Himeno, K.; Nomoto, K.

    1987-01-01

    The sequential differentiation patterns of thymocyte were observed with cell surface phenotypes and the expression of T cell antigen receptor in 800 rad irradiated adult mice. Thymus was severely reduced in size and cell number by day 5 after whole body irradiation and rapidly recovered from day 7 to day 14. Surface marker analysis on day 5 after irradiation showed thymocytes with Thy-1low L3T4+/Lyt-2- dominantly existed and suggested that these cells were radioresistant-survived cells. On the other hand, thymocytes on day 7 were composed of a large number of Thy-1high L3T4+/Lyt-2+ blast-like cells and a relatively high proportion of Thy-1high L3T4-/Lyt-2- cells which expressed a large amount of gamma-chain gene messages but scarcely any alpha- and beta-chain gene messages similar to the fetal thymocytes. On day 14, thymocytes were composed mostly of Thy-1high H-2low L3T4+/Lyt-2+ subpopulation which expressed a remarkably low level of gamma-chain gene messages, and high levels of alpha- and beta-chain transcripts analogous to those of normal adult thymus. Taken together, intrathymic radioresistent stem cells for T thymocytes seem to proliferate and differentiate after irradiation with the same pattern as was seen in a fetal thymus development

  16. PROFIBUS-DP. Fundamentals and hints for users; PROFIBUS-DP. Grundlagen, Tips und Tricks fuer Anwender

    Energy Technology Data Exchange (ETDEWEB)

    Popp, M.

    1998-04-01

    PROFIBUS-DP is one of the most common field buses for industrial communication. The author provides competent help in using this technology. He describes 32 of the most common errors found in certification tests and shows how they can be remedies. Fundamentals and enhancements of PROFIBUS-DP are presented. PROFIBUS-DP telegrams for data interpretation are discussed at length, as are extensive GSD files. Particular emphasis is given to the projecting and commissioning of a system and practical guides for installation. (orig.) [Deutsch] PROFIBUS-DP gehoert zu den am haeufigsten eingesetzten Feldbussen fuer die industrielle Kommunikation. Der Autor bietet in diesem umfassenden Werk kompetente Unterstuetzung bei der Anwendung dieser Feldbustechnik. Es werden 32 der haeufigsten Fehler, die bei Zertifizierungstests festgestellt wurden, beschrieben und Wege zu ihrer Behebung dargestellt. Die Grundlagen von PROFIBUS-DP werden erlaeutert und die PROFIBUS-Erweiterungen vorgestellt. PROFIBUS-DP-Telegramme zur Interpretation des Datenverkehrs werden genauso detailliert erklaert wie eine umfangreiche GSD-Datei. Ein besonderer Schwerpunkt ist die Projektierung und Inbetriebnahme einer Anlage und den praxisgerechten Aufbaurichtlinien gewidmet. (orig./GL)

  17. Study in regularities in the formation of double stranded DNA breaks in irradiated rat thymocytes

    International Nuclear Information System (INIS)

    Ivannik, B.P.; ProskuryakoV, S.Ya.; Ryabchenko, N.I.

    1979-01-01

    Using low-gradient viscosimetry of neutral detergent nuclear lysates a study was made of postradiation changes in the molecular weight of double-stranded DNA of thymocytes. It was established that 375 eV are needed for one double-stranded break to appear, and a dose of 1 rad is required for 0.275 double-stranded break to occur at the site of DNA with m.w. 10 12 dalton. The repair of double-stranded breaks is only observed when rats are exposed to a dose of 500 R. It is assumed that the absence of repair of double-stranded DNA breaks and the presence of secondary postradiation degradation of DNA are responsible for thymocyte death

  18. Recombinatorial biases and convergent recombination determine interindividual TCRβ sharing in murine thymocytes.

    Science.gov (United States)

    Li, Hanjie; Ye, Congting; Ji, Guoli; Wu, Xiaohui; Xiang, Zhe; Li, Yuanyue; Cao, Yonghao; Liu, Xiaolong; Douek, Daniel C; Price, David A; Han, Jiahuai

    2012-09-01

    Overlap of TCR repertoires among individuals provides the molecular basis for public T cell responses. By deep-sequencing the TCRβ repertoires of CD4+CD8+ thymocytes from three individual mice, we observed that a substantial degree of TCRβ overlap, comprising ∼10-15% of all unique amino acid sequences and ∼5-10% of all unique nucleotide sequences across any two individuals, is already present at this early stage of T cell development. The majority of TCRβ sharing between individual thymocyte repertoires could be attributed to the process of convergent recombination, with additional contributions likely arising from recombinatorial biases; the role of selection during intrathymic development was negligible. These results indicate that the process of TCR gene recombination is the major determinant of clonotype sharing between individuals.

  19. β-endorphin modulation of mitogen-stimulated calcium uptake by rat thymocytes

    International Nuclear Information System (INIS)

    Hemmick, L.M.; Bidlack, J.M.

    1987-01-01

    Lymphocytes stimulated by mitogens or antigens exhibit an enhanced calcium uptake early in the proliferation or activation response. Modulation of this calcium uptake results in alterations of proliferation and immunocompetence. β-endorphin and other opioids affect several parameters of lymphocyte competence. Limited data are available concerning the mechanism(s) of these effects. This study examines whether a possible opioid mechanism is the modification of the early calcium influx into stimulated lymphocytes. The time course of both concanavalin A (Con A) and phytohemagglutinin (PHA)-stimulated 45 Ca 2+ uptake into thymocytes was characterized to determine the optimal time for testing the effects of opioids. Β-Endorphin 1-31 significantly enhanced Con A-stimulated 45 Ca 2+ uptake into rat thymocytes. This peptide had no significant effect on PHA-simulated 45 Ca 2+ uptake or on basal thymocyte 45 Ca 2+ flux. The β/sub h/-endorphin stimulatory effect was titratable in the range of 0.1 nM to 10 μM. Naloxone did not reverse the enhancement. Met-enkephalinamide and other opioid agonists did not duplicate the stimulatory effect. Thus, the β/sub h/-endorphin 1-31 enhancement of Con A-stimulated 45 Ca 2+ uptake by rat thymocytes does not operate via classical opioid receptor mechanisms. β/sub h/-endorphin 1-31 appears to be acting on a subset of T cells that are responsive to Con A but not to PHA. 30 references, 4 figures, 1 table

  20. beta. -endorphin modulation of mitogen-stimulated calcium uptake by rat thymocytes

    Energy Technology Data Exchange (ETDEWEB)

    Hemmick, L.M.; Bidlack, J.M.

    1987-10-19

    Lymphocytes stimulated by mitogens or antigens exhibit an enhanced calcium uptake early in the proliferation or activation response. Modulation of this calcium uptake results in alterations of proliferation and immunocompetence. ..beta..-endorphin and other opioids affect several parameters of lymphocyte competence. Limited data are available concerning the mechanism(s) of these effects. This study examines whether a possible opioid mechanism is the modification of the early calcium influx into stimulated lymphocytes. The time course of both concanavalin A (Con A) and phytohemagglutinin (PHA)-stimulated /sup 45/Ca/sup 2 +/ uptake into thymocytes was characterized to determine the optimal time for testing the effects of opioids. BETA-Endorphin 1-31 significantly enhanced Con A-stimulated /sup 45/Ca/sup 2 +/ uptake into rat thymocytes. This peptide had no significant effect on PHA-simulated /sup 45/Ca/sup 2 +/ uptake or on basal thymocyte /sup 45/Ca/sup 2 +/ flux. The ..beta../sub h/-endorphin stimulatory effect was titratable in the range of 0.1 nM to 10 ..mu..M. Naloxone did not reverse the enhancement. Met-enkephalinamide and other opioid agonists did not duplicate the stimulatory effect. Thus, the ..beta../sub h/-endorphin 1-31 enhancement of Con A-stimulated /sup 45/Ca/sup 2 +/ uptake by rat thymocytes does not operate via classical opioid receptor mechanisms. ..beta../sub h/-endorphin 1-31 appears to be acting on a subset of T cells that are responsive to Con A but not to PHA. 30 references, 4 figures, 1 table.

  1. Apoptosis in thymocytes and lymphoma cells induced by neutrons and X-rays

    International Nuclear Information System (INIS)

    Ohyama, Harumi; Koike, Sachiko; Ando, Kouichi

    1993-01-01

    Apoptosis is a distinctive mode of programmed cell death with characteristic morphological and biochemical features. The cells undergoing apoptosis display shrinkage, chromatin condensation and internucleosomal breakage of DNA. The cell death is a process through which organisms get rid of unwanted cells. Thymocytes are highly radiosensitive, and undergo typical apoptosis within a few hours after the exposure to X-ray. Also extremely radiosensitive thymic lymphoma cells have been found. The effect of 30 MeV NIRS fast neutrons on the induction of apoptosis in thymocytes and thymoma cells was examined in comparison with that of X-ray. Although apoptotic cells increased gradually with time, the apoptotic process proceeded rapidly in individual cells after the onset. By the measurement of cell size distribution, the appearance of a distinct apoptotic cell peak was observed. In the case of neutron irradiation on SCA1 thymic lymphoma cells, the method for counting apoptotic cells based on chromatin condensation was developed. The cell volume reduction of thymocytes, the dose survival curves and the induction of apoptosis in SCA1 are reported. (K.I.)

  2. Somatodendritic and excitatory postsynaptic distribution of neuron-type dystrophin isoform, Dp40, in hippocampal neurons.

    Science.gov (United States)

    Fujimoto, Takahiro; Itoh, Kyoko; Yaoi, Takeshi; Fushiki, Shinji

    2014-09-12

    The Duchenne muscular dystrophy (DMD) gene produces multiple dystrophin (Dp) products due to the presence of several promoters. We previously reported the existence of a novel short isoform of Dp, Dp40, in adult mouse brain. However, the exact biochemical expression profile and cytological distribution of the Dp40 protein remain unknown. In this study, we generated a polyclonal antibody against the NH2-terminal region of the Dp40 and identified the expression profile of Dp40 in the mouse brain. Through an analysis using embryonic and postnatal mouse cerebrums, we found that Dp40 emerged from the early neonatal stages until adulthood, whereas Dp71, an another Dp short isoform, was highly detected in both prenatal and postnatal cerebrums. Intriguingly, relative expressions of Dp40 and Dp71 were prominent in cultured dissociated neurons and non-neuronal cells derived from mouse hippocampus, respectively. Furthermore, the immunocytological distribution of Dp40 was analyzed in dissociated cultured neurons, revealing that Dp40 is detected in the soma and its dendrites, but not in the axon. It is worthy to note that Dp40 is localized along the subplasmalemmal region of the dendritic shafts, as well as at excitatory postsynaptic sites. Thus, Dp40 was identified as a neuron-type Dp possibly involving dendritic and synaptic functions. Copyright © 2014 Elsevier Inc. All rights reserved.

  3. Johnson - Cook Strength Models for Mild and DP 590 Steels

    International Nuclear Information System (INIS)

    Vedantam, K.; Brar, N. S.; Bajaj, D.; Hill, S.

    2006-01-01

    Automotive steels, Mild and Dual Phase590 (DP590) are characterized in tension at room temperature, using the quasi-static and split Hopkinson bar techniques at various strain rates ranging from ∼10-3/s to ∼1800/s. Tension stress-strain data for both the steels are analyzed to determine the Johnson-Cook Strength model constants, J-C strength model constants for mild steel are A=217 MPa, B = 234 MPa, n = 0.643 and C = 0.076 and for DP590 steel are A = 430 MPa, B = 824 MPa, n = 0.510 and C = 0.017. Higher value of strain rate sensitivity constant C for mild steel (0.076) compared to DP 590 (0.017) is also reflected in the stress- strain data at various strain rates

  4. DOE Defense Program (DP) safety programs. Final report, Task 003

    International Nuclear Information System (INIS)

    1998-01-01

    The overall objective of the work on Task 003 of Subcontract 9-X52-W7423-1 was to provide LANL with support to the DOE Defense Program (DP) Safety Programs. The effort included the identification of appropriate safety requirements, the refinement of a DP-specific Safety Analysis Report (SAR) Format and Content Guide (FCG) and Comprehensive Review Plan (CRP), incorporation of graded approach instructions into the guidance, and the development of a safety analysis methodologies document. All tasks which were assigned under this Task Order were completed. Descriptions of the objectives of each task and effort performed to complete each objective is provided here

  5. DESIGN OF BUILDING AUTOMATION BASED ON PROFIBUS-DP NETWORK

    Directory of Open Access Journals (Sweden)

    Cemal YILMAZ

    2006-02-01

    Full Text Available In this study, a building automation has been designed by using the Profibus DP (Process Field Bus- Decentralized Periphery network. In the study; fire alarm, thief alarm, lighting, power, humidity and temperature control have been implemented. The data from building has been transmitted to the Profibus-DP network via control point located on the flats. The data taken from the building has been collected in the main control unit to achieve overall control of the system. The work has provided an optimum efficiency in energy consumption, control of power, security, temperature and humidity.

  6. Diethylstilbestrol alters positive and negative selection of T cells in the thymus and modulates T-cell repertoire in the periphery

    International Nuclear Information System (INIS)

    Brown, Nicole; Nagarkatti, Mitzi; Nagarkatti, Prakash S.

    2006-01-01

    Prenatal exposure to diethylstilbestrol (DES) is known to cause altered immune functions and increased susceptibility to autoimmune disease in humans. In the current study, we investigated the effects of DES on T-cell differentiation in the thymus using the HY-TCR transgenic (Tg) mouse model in which the female mice exhibit positive selection of T cells bearing the Tg TCR, while the male mice show negative selection of such T cells. In female HY-TCR-Tg mice, exposure to DES showed more pronounced decrease in thymic cellularity when compared to male mice. Additionally, female mice also showed a significant decrease in the proportion of double-positive (DP) T cells in the thymus and HY-TCR-specific CD8 + T cells in the periphery. Male mice exhibiting negative selection also showed decreased thymic cellularity following DES exposure. Moreover, the male mice showed increased proportion of double-negative (DN) T cells in the thymus and decreased proportion of CD8 + T cells. The density of expression of HY-TCR on CD8 + cells was increased following DES exposure in both females and males. Finally, the proliferative response of thymocytes to mitogens and peripheral lymph node T cells to male H-Y antigen was significantly altered in female and male mice following DES treatment. Taken together, these data suggest that DES alters T-cell differentiation in the thymus by interfering with positive and negative selection processes, which in turn modulates the T-cell repertoire in the periphery

  7. Biphasic stimulation of cellular calcium concentration by 3,5,3'-triiodothyronine in rat thymocytes

    International Nuclear Information System (INIS)

    Segal, J.

    1988-01-01

    3,5,3'-Triiodothyronine (T 3 ) produced a rapid and transient increase in 45 Ca uptake and cytoplasmic free calcium concentration in rat thymocytes, which is the most rapid effect of T 3 in this system. This effect was manifested in cells suspended in medium containing 1 mM calcium. The T 3 effect on 45 Ca uptake was evident at 15-30 s, reached maximum at 30-60 s, and returned to control values at 5 min. The T 3 effect on cytoplasmic free calcium concentration was seen after 30 s, reached maximum at 7 min, and returned to control values after 24 min. In cells suspended in Ca 2+ -free medium, T 3 produced a similar rapid increase in 45 Ca uptake, which was sustained for at least 60 min, but T 3 failed to change cytoplasmic free calcium concentration. Alprenolol (10 μM) blocked the stimulatory effects of T 3 on these two functions in a similar fashion. From these results, the authors suggest that in rat thymocytes T 3 influences cellular calcium economy through a biphasic mechanism in which T 3 first increases calcium uptake which, in turn, if followed by a release of calcium from intracellular pool(s), resulting in a further increase in cytoplasmic free calcium concentration and the activation of Ca 2+ -regulated systems. Moreover, the present study provides further support for the postulate that in the rat thymocyte calcium serves as the first messenger for the plasma membrane-mediated stimulatory effects of T 3 on several metabolic functions

  8. Radiation effect on the activity of alkaline DNA-ase in rat thymocytes

    International Nuclear Information System (INIS)

    Ivannik, B.P.; Proskuryakov, S.Ya.; Golubeva, R.V.; Ryabchenko, N.I.

    1978-01-01

    Using a modified viscosimetric method changes in the activity of alkaline DNAase were registered in thymocytes of irradiated rats. Four hours following irradiation, the activity of alkaline DNAase was 2.17, 3.33 and 4.50 times as high after doses of 500, 900 and 3000 R, respectively. Six hours after exposure the activity was 4.21, 6.18 and 7.83 times as high at doses of 500, 900 and 3000 R, respectively. At a dose of 3000 R, the activity of DNAase was practically invariable during the first three hours after irradiation. After 3 hours, its activity was 1.32 time as high

  9. The HLA-DP polymorphism in Denmark investigated by local and international PLT reagents. Definition of two "new" DP antigens

    DEFF Research Database (Denmark)

    Ødum, Niels; Hartzman, R; Jakobsen, B K

    1986-01-01

    Lymphocytes from highly selected donors were primed for 10 days and subsequently bulk-expanded in IL 2 (TCGF) containing cultures. Two well-discriminatory PLT (CDP = Copenhagen DP) reagents against each of the DPw1-w6 specificities and one against each of the two "new" specificities, CDP4s...

  10. Microstructure and mechanical properties of resistance spot welded dissimilar thickness DP780/DP600 dual-phase steel joints

    International Nuclear Information System (INIS)

    Zhang, Hongqiang; Wei, Ajuan; Qiu, Xiaoming; Chen, Jianhe

    2014-01-01

    Highlights: • We examine changes of microstructure of dissimilar thickness DP600/DP780 joints. • The hardness profile of RSW joints can be predicted by the equation. • Failure modes, peak load and energy describes the mechanical properties of joints. • The nugget diameter is the key factor of transition between the failure modes. - Abstract: In this study, resistance spot welding (RSW) experiments were performed in order to evaluate the microstructure and mechanical properties of single-lap joints between DP780 and DP600. The results show that the weld joints consist of three regions including base metal (BM), heat affected zone (HAZ) and fusion zone (FZ). The grain size and martensite volume fractions increase in the order of BM, HAZ and FZ. The hardness in the FZ is significantly higher than hardness of base metals. Tensile properties of the joints were described in terms of the failure modes and static load-carrying capabilities. Two distinct failure modes were observed during the tensile shear test of the joints: interfacial failure (IF) and pullout failure (PF). The FZ size plays a dominate role in failure modes of the joints

  11. Multirate control with incomplete information over Profibus-DP network

    Science.gov (United States)

    Salt, J.; Casanova, V.; Cuenca, A.; Pizá, R.

    2014-07-01

    When a process field bus-decentralized peripherals (Profibus-DP) network is used in an industrial environment, a deterministic behaviour is usually claimed. However, due to some concerns such as bandwidth limitations, lack of synchronisation among different clocks and existence of time-varying delays, a more complex problem must be faced. This problem implies the transmission of irregular and, even, random sequences of incomplete information. The main consequence of this issue is the appearance of different sampling periods at different network devices. In this paper, this aspect is checked by means of a detailed Profibus-DP timescale study. In addition, in order to deal with the different periods, a delay-dependent dual-rate proportional-integral-derivative control is introduced. Stability for the proposed control system is analysed in terms of linear matrix inequalities.

  12. PROFIBUS-DP AĞ TABANLI BİNA OTOMASYONU TASARIMI

    OpenAIRE

    Cemal YILMAZ; İ. Serkan ÜNCÜ

    2006-01-01

    Bu çalışmada, Profibus-DP ağı kullanılarak bir Bina Otomasyonu tasarlanmıştır. Tasarımda; yangın algılama, hırsız algılama, aydınlatma, güç, nem ve sıcaklık denetimi gerçekleştirilmiştir. Binada veriler katlarda bulunan denetim noktalarında toplanarak arabirimler vasıtasıyla Profibus-DP ağına aktarılmaktadır. Binadan alınan veriler ana denetim biriminde toplanarak binanın tamamı denetim altına alınmıştır. Çalışma sonucunda enerji tasarrufu, güç denetimi, güvenlik, ısı ve nem denetiminde optim...

  13. Peptide binding predictions for HLA DR, DP and DQ molecules

    DEFF Research Database (Denmark)

    Wang, P.; Sidney, J.; Kim, Y.

    2010-01-01

    a significant gap in knowledge as HLA DP and DQ molecules are presumably equally important, and have only been studied less because they are more difficult to handle experimentally. RESULTS: In this study, we aimed to narrow this gap by providing a large scale dataset of over 17,000 HLA-peptide binding...... affinities for a set of 11 HLA DP and DQ alleles. We also expanded our dataset for HLA DR alleles resulting in a total of 40,000 MHC class II binding affinities covering 26 allelic variants. Utilizing this dataset, we generated prediction tools utilizing several machine learning algorithms and evaluated...... include all training data for maximum performance. 4) The recently developed NN-align prediction method significantly outperformed all other algorithms, including a naïve consensus based on all prediction methods. A new consensus method dropping the comparably weak ARB prediction method could outperform...

  14. BEHAVIOR OF STEEL DP 600 UNDER DYNAMIC CONDITIONS

    Directory of Open Access Journals (Sweden)

    Miroslav Német

    2014-01-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Dynamic tensile testing of sheet steels is becoming more important. Experimental dynamic tensile technique is depending on the strain rate. For experiments was used two testing method servo hydraulic and single bar method. Experiments was realized on steel grade DP 600. Steel were performed and evaluated static and dynamic tests. Was investigated substructure in static and dynamic loading conditions.

  15. The complete experiment for backward elastic dp scattering

    International Nuclear Information System (INIS)

    Rekalo, M.P.; Piskunov, N.M.; Sitnik, I.M.

    1996-01-01

    The problem of the complete experiment in backward elastic dp scattering is analyzed. All effects due to polarization of one or two initial and one of secondary particles are considered. It is shown that the minimal set of measurements allowing to reconstruct each of four amplitudes describing this process does not comprise too complicated experiments and is quite realistic nowadays. The geography of realization of the complete experiment is briefly reviewed. 21 refs

  16. Aztec castles and the dP3 quiver

    International Nuclear Information System (INIS)

    Leoni, Megan; Musiker, Gregg; Neel, Seth; Turner, Paxton

    2014-01-01

    Bipartite, periodic, planar graphs known as brane tilings can be associated to a large class of quivers. This paper will explore new algebraic properties of the well-studied del Pezzo 3 (dP3) quiver and geometric properties of its corresponding brane tiling. In particular, a factorization formula for the cluster variables arising from a large class of mutation sequences (called τ-mutation sequences) is proven; this factorization also gives a recursion on the cluster variables produced by such sequences. We can realize these sequences as walks in a triangular lattice using a correspondence between the generators of the affine symmetric group A 2 -tilde and the mutations which generate τ-mutation sequences. Using this bijection, we obtain explicit formulae for the cluster that corresponds to a specific alcove in the lattice. With this lattice visualization in mind, we then express each cluster variable produced in a τ-mutation sequence as the sum of weighted perfect matchings of a new family of subgraphs of the dP3 brane tiling, which we call Aztec castles. Our main result generalizes previous work on a certain mutation sequence on the dP3 quiver in Zhang (2012 Cluster Variables and Perfect Matchings of Subgraphs of the dP3 Lattice http://www.math.umn.edu/~/REU/Zhang2012.pdf), and forms part of the emerging story in combinatorics and theoretical high energy physics relating cluster variables to subgraphs of the associated brane tiling. This article is part of a special issue of Journal of Physics A: Mathematical and Theoretical devoted to ‘Cluster algebras in mathematical physics’. (paper)

  17. 75 FR 10317 - DHL Global Forwarding, A Subsidiary of DP DHL, Finance and Accounting Divisions, Including...

    Science.gov (United States)

    2010-03-05

    ...,857B; TA-W-70,857C; TA-W-70,857D] DHL Global Forwarding, A Subsidiary of DP DHL, Finance and Accounting... Global Forwarding, A Subsidiary of DP DHL, Finance and Accounting Divisions, Including Workers Whose... Subsidiary of DP DHL Finance and Accounting Divisions, Including Workers Whose Wages Are Reported to Danzas...

  18. 6-methylprednisolone does not impair anti-thymocyte globulin (ATG) immunosuppressive activity in non-human primates

    NARCIS (Netherlands)

    Preville, [No Value; Sick, E; Beauchard, S; Ossevoort, M; Tiollier, J; Revillard, JP; Jonker, Margreet

    2001-01-01

    Background: Induction treatments with anti-thymocyte globulin (ATG) in solid organ transplantation may enhance the efficacy of maintenance immunosuppressive therapy. Since ATG can trigger Fas (CD95) mediated T cell apoptosis, a process antagonized in vitro by corticosteroids, an important issue is

  19. Adaptation of the DP 50 dust meter for measuring dust content under isokinetic conditions

    Energy Technology Data Exchange (ETDEWEB)

    Vitek, J.; Novak, L.

    1985-03-01

    The DP 50 dust meter, developed by the Scientific Coal Research Institute Ostrava-Radvanice, is used for measuring dust content in the air in underground coal mines. Two versions of the system are used: a type developed in 1970 which is placed in a vertical position and used to measure the content of respirable coal particles in the air; and a type developed in 1983 for isokinetic measurement of dust content in the air. The latter is equipped with 8 cone-shaped adapters (with differing size and dimensions of the cone inlet adjusted to air flow rates from 0.25 to 8.00 m/s). Specifications of the 8 adapters are given in a table. The 1983 version of the DP 50 is placed in a horizontal position with the dust meter axis parallel to the direction of air flow ventilating a mine working. Recommendations for installation of dust meters in underground workings and effects of installation on measurement accuracy are discussed. 16 references.

  20. Prenatal cadmium exposure dysregulates sonic hedgehog and Wnt/β-catenin signaling in the thymus resulting in altered thymocyte development

    International Nuclear Information System (INIS)

    Hanson, Miranda L.; Brundage, Kathleen M.; Schafer, Rosana; Tou, Janet C.; Barnett, John B.

    2010-01-01

    Cadmium (Cd) is both an environmental pollutant and a component of cigarette smoke. Although evidence demonstrates that adult exposure to Cd causes changes in the immune system, there are limited reports in the literature of immunomodulatory effects of prenatal exposure to Cd. The sonic hedgehog (Shh) and Wnt/β-catenin pathways are required for thymocyte maturation. Several studies have demonstrated that Cd exposure affects these pathways in different organ systems. This study was designed to investigate the effect of prenatal Cd exposure on thymocyte development, and to determine if these effects were linked to dysregulation of Shh and Wnt/β-catenin pathways. Pregnant C57Bl/6 mice were exposed to an environmentally relevant dose (10 ppm) of Cd throughout pregnancy and effects on the thymus were assessed on the day of birth. Thymocyte phenotype was determined by flow cytometry. A Gli:luciferase reporter cell line was used to measure Shh signaling. Transcription of target genes and translation of key components of both signaling pathways were assessed using real-time RT-PCR and western blot, respectively. Prenatal Cd exposure increased the number of CD4 + cells and a subpopulation of double-negative cells (DN; CD4 - CD8 - ), DN4 (CD44 - CD25 - ). Shh and Wnt/β-catenin signaling were both decreased in the thymus. Target genes of Shh (Patched1 and Gli1) and Wnt/β-catenin (c-fos, and c-myc) were affected differentially among thymocyte subpopulations. These findings suggest that prenatal exposure to Cd dysregulates two signaling pathways in the thymus, resulting in altered thymocyte development.

  1. DN2 Thymocytes Activate a Specific Robust DNA Damage Response to Ionizing Radiation-Induced DNA Double-Strand Breaks

    Directory of Open Access Journals (Sweden)

    Irene Calvo-Asensio

    2018-06-01

    Full Text Available For successful bone marrow transplantation (BMT, a preconditioning regime involving chemo and radiotherapy is used that results in DNA damage to both hematopoietic and stromal elements. Following radiation exposure, it is well recognized that a single wave of host-derived thymocytes reconstitutes the irradiated thymus, with donor-derived thymocytes appearing about 7 days post BMT. Our previous studies have demonstrated that, in the presence of donor hematopoietic cells lacking T lineage potential, these host-derived thymocytes are able to generate a polyclonal cohort of functionally mature peripheral T cells numerically comprising ~25% of the peripheral T cell pool of euthymic mice. Importantly, we demonstrated that radioresistant CD44+ CD25+ CD117+ DN2 progenitors were responsible for this thymic auto-reconstitution. Until recently, the mechanisms underlying the radioresistance of DN2 progenitors were unknown. Herein, we have used the in vitro “Plastic Thymus” culture system to perform a detailed investigation of the mechanisms responsible for the high radioresistance of DN2 cells compared with radiosensitive hematopoietic stem cells. Our results indicate that several aspects of DN2 biology, such as (i rapid DNA damage response (DDR activation in response to ionizing radiation-induced DNA damage, (ii efficient repair of DNA double-strand breaks, and (iii induction of a protective G1/S checkpoint contribute to promoting DN2 cell survival post-irradiation. We have previously shown that hypoxia increases the radioresistance of bone marrow stromal cells in vitro, at least in part by enhancing their DNA double-strand break (DNA DSB repair capacity. Since the thymus is also a hypoxic environment, we investigated the potential effects of hypoxia on the DDR of DN2 thymocytes. Finally, we demonstrate for the first time that de novo DN2 thymocytes are able to rapidly repair DNA DSBs following thymic irradiation in vivo.

  2. Enhancement of cell-cell contact by a nonmitogenic lectin increases blastogenic response and IL-2 release by mitogen-stimulated mouse thymocytes.

    Science.gov (United States)

    Favero, J; Marti, J; Dornand, J; Bonnafous, J C; Mani, J C

    1986-03-01

    We have examined the influence of peanut agglutinin (PNA), a lectin which agglutinates but does not stimulate mouse thymocytes, on the responsiveness of these cells to concanavalin A (Con A) or galactose oxidase stimulation. Binding low amounts of PNA on unseparated mouse thymocytes pretreated with neuraminidase highly enhances the mitogenic response and the level of interleukin 2 release in the culture medium upon Con A stimulation. We have shown that PNA present on the cell surface acts as a crosslinking agent which favors intercellular binding between accessory cells (macrophages) and thymocytes, leading through this enhanced cooperation by cell-cell contact to an enhanced blastogenic response.

  3. HLA-DP antigens in HIV-infected individuals

    DEFF Research Database (Denmark)

    Ødum, Niels; Georgsen, J; Fugger, L

    1991-01-01

    We studied the distribution of HLA-DP antigens in 74 HIV-infected Danish homosexual men and 188 ethnically matched healthy individuals, using the primed lymphocyte typing (PLT) technique. Forty of the patients developed AIDS within 3 years after diagnosis, whereas the remaining 34 were healthy...... or had only minor symptoms for 3 years or more (median observation time was 42 months). HLA-DPwl seemed to be decreased (relative risk = 0.3) in AIDS patients (5.0 per cent) when compared to patients with minor symptoms (14.7 per cent) and healthy controls (14.9 per cent). These differences were, however...

  4. Rotating and orbiting strings in Dp-brane background

    Energy Technology Data Exchange (ETDEWEB)

    Biswas, Sagar; Panigrahi, Kamal L. [Department of Physics, Indian Institute of Technology Kharagpur,721302, Kharagpur (India)

    2015-03-04

    We probe the open fundamental strings in Dp-brane (p=1, 3, 5) backgrounds and find new classes of rotating and orbiting string solutions. We show that for various worldsheet embedding ansatz we get solutions of the string equations of motion that correspond to the well known giant magnon and single spikes, in addition to few new solutions corresponding to the orbiting strings. We make a systematic study of both rigidly rotating and orbiting strings in D1, D3 and D5-brane backgrounds.

  5. Los Alamos DP West Plutonium Facility decontamination project

    International Nuclear Information System (INIS)

    Garde, R.; Cox, E.J.; Valentine, A.M.

    1982-01-01

    The DP West Plutonium Facility operated by the Los Alamos National Laboratory, Los Alamos, New Mexico, was decontaminated between April 1978 and April 1981. The facility was constructed in 1944 to 1945 to produce plutonium metal and fabricate parts for nuclear weapons. It was continually used as a plutonium processing and research facility until mid-1978. Decontamination operations included dismantling and removing gloveboxes and conveyor tunnels; removing process systems, utilities, and exhaust ducts; and decontaminating all remaining surfaces. This report describes glovebox and conveyor tunnel separations, decontamination techniques, health and safety considerations, waste management procedures, and costs of the operation

  6. Identification of Dynamically Positioned Ships

    Directory of Open Access Journals (Sweden)

    Thor I. Fossen

    1996-04-01

    Full Text Available Todays model-based dynamic positioning (DP systems require that the ship and thruster dynamics are known with some accuracy in order to use linear quadratic optical control theory. However, it is difficult to identify the mathematical model of a dynamically posititmed (DP ship since the ship is not persistently excited under DP. In addition the ship parameter estimation problem is nonlinear and multivariable with only position and thruster state measurements available for parameter estimation. The process and measurement noise must also be modeled in order to avoid parameter drift due to environmental disturbances and sensor failure. This article discusses an off-line parallel extended Kalman filter (EKF algorithm utilizing two measurement series in parallel to estimate the parameters in the DP ship model. Full-scale experiments with a supply vessel are used to demonstrate the convergence and robustness of the proposed parameter estimator.

  7. The DP-1 transcription factor is required for keratinocyte growth and epidermal stratification.

    Science.gov (United States)

    Chang, Wing Y; Bryce, Dawn M; D'Souza, Sudhir J A; Dagnino, Lina

    2004-12-03

    The epidermis is a stratified epithelium constantly replenished through the ability of keratinocytes in its basal layer to proliferate and self-renew. The epidermis arises from a single-cell layer ectoderm during embryogenesis. Large proliferative capacity is central to ectodermal cell and basal keratinocyte function. DP-1, a heterodimeric partner of E2F transcription factors, is highly expressed in the ectoderm and all epidermal layers during embryogenesis. To investigate the role of DP-1 in epidermal morphogenesis, we inhibited DP-1 activity through exogenous expression of a dominant-negative mutant (dnDP-1). Expression of the dnDP-1 mutant interferes with binding of E2F/DP-1 heterodimers to DNA and inhibits DNA replication, as well as cyclin A mRNA and protein expression. Chromatin immunoprecipitation analysis demonstrated that the cyclin A promoter is predominantly bound in proliferating keratinocytes by complexes containing E2F-3 and E2F-4. Thus, the mechanisms of decreased expression of cyclin A in the presence of dnDP-1 seem to involve inactivation of DP-1 complexes containing E2F-3 and E2F-4. To assess the consequences on epidermal morphogenesis of inhibiting DP-1 activity, we expressed dnDP-1 in rat epithelial keratinocytes in organotypic culture and observed that DP-1 inhibition negatively affected stratification of these cells. Likewise, expression of dnDP-1 in embryonic ectoderm explants produced extensive disorganization of subsequently formed epidermal basal and suprabasal layers, interfering with normal epidermal formation. We conclude that DP-1 activity is required for normal epidermal morphogenesis and ectoderm-to-epidermis transition.

  8. PROFIBUS-DP AĞ TABANLI BİNA OTOMASYONU TASARIMI

    Directory of Open Access Journals (Sweden)

    Cemal YILMAZ

    2006-02-01

    Full Text Available Bu çalışmada, Profibus-DP ağı kullanılarak bir Bina Otomasyonu tasarlanmıştır. Tasarımda; yangın algılama, hırsız algılama, aydınlatma, güç, nem ve sıcaklık denetimi gerçekleştirilmiştir. Binada veriler katlarda bulunan denetim noktalarında toplanarak arabirimler vasıtasıyla Profibus-DP ağına aktarılmaktadır. Binadan alınan veriler ana denetim biriminde toplanarak binanın tamamı denetim altına alınmıştır. Çalışma sonucunda enerji tasarrufu, güç denetimi, güvenlik, ısı ve nem denetiminde optimum verim sağlanmıştır.

  9. Visualizing molecular profiles of glioblastoma with GBM-BioDP.

    Directory of Open Access Journals (Sweden)

    Orieta Celiku

    Full Text Available Validation of clinical biomarkers and response to therapy is a challenging topic in cancer research. An important source of information for virtual validation is the datasets generated from multi-center cancer research projects such as The Cancer Genome Atlas project (TCGA. These data enable investigation of genetic and epigenetic changes responsible for cancer onset and progression, response to cancer therapies, and discovery of the molecular profiles of various cancers. However, these analyses often require bulk download of data and substantial bioinformatics expertise, which can be intimidating for investigators. Here, we report on the development of a new resource available to scientists: a data base called Glioblastoma Bio Discovery Portal (GBM-BioDP. GBM-BioDP is a free web-accessible resource that hosts a subset of the glioblastoma TCGA data and enables an intuitive query and interactive display of the resultant data. This resource provides visualization tools for the exploration of gene, miRNA, and protein expression, differential expression within the subtypes of GBM, and potential associations with clinical outcome, which are useful for virtual biological validation. The tool may also enable generation of hypotheses on how therapies impact GBM molecular profiles, which can help in personalization of treatment for optimal outcome. The resource can be accessed freely at http://gbm-biodp.nci.nih.gov (a tutorial is included.

  10. Thruster allocation for dynamical positioning

    NARCIS (Netherlands)

    Poppe, K.; van den Berg, J.B.; Blank, E.; Archer, C.; Redeker, M.; Kutter, M.; Hemker, P.

    2010-01-01

    Positioning a vessel at a fixed position in deep water is of great importance when working offshore. In recent years a Dynamical Positioning (DP) system was developed at Marin [2]. After the measurement of the current position and external forces (like waves, wind etc.), each thruster of the vessel

  11. Indirect presentation in the thymus limits naive and regulatory T-cell differentiation by promoting deletion of self-reactive thymocytes.

    Science.gov (United States)

    Yap, Jin Yan; Wirasinha, Rushika C; Chan, Anna; Howard, Debbie R; Goodnow, Christopher C; Daley, Stephen R

    2018-02-07

    Acquisition of T-cell central tolerance involves distinct pathways of self-antigen presentation to thymocytes. One pathway termed indirect presentation requires a self-antigen transfer step from thymic epithelial cells (TECs) to bone marrow-derived cells before the self-antigen is presented to thymocytes. The role of indirect presentation in central tolerance is context-dependent, potentially due to variation in self-antigen expression, processing and presentation in the thymus. Here, we report experiments in mice in which TECs expressed a membrane-bound transgenic self-antigen, hen egg lysozyme (HEL), from either the insulin (insHEL) or thyroglobulin (thyroHEL) promoter. Intrathymic HEL expression was less abundant and more confined to the medulla in insHEL mice compared with thyroHEL mice. When indirect presentation was impaired by generating mice lacking MHC class II expression in bone marrow-derived antigen-presenting cells, insHEL-mediated thymocyte deletion was abolished, whereas thyroHEL-mediated deletion occurred at a later stage of thymocyte development and Foxp3 + regulatory T-cell differentiation increased. Indirect presentation increased the strength of T-cell receptor signalling that both self-antigens induced in thymocytes, as assessed by Helios expression. Hence, indirect presentation limits the differentiation of naive and regulatory T cells by promoting deletion of self-reactive thymocytes. © 2018 John Wiley & Sons Ltd.

  12. During Stably Suppressive Antiretroviral Therapy Integrated HIV-1 DNA Load in Peripheral Blood is Associated with the Frequency of CD8 Cells Expressing HLA-DR/DP/DQ

    Directory of Open Access Journals (Sweden)

    Alessandra Ruggiero

    2015-09-01

    Conclusions: The observed positive association between integrated HIV-1 DNA load and frequency of CD8+DR/DP/DQ+ cells indicates that a close correlation between HIV persistence and immune activation continues during consistently suppressive therapy. The inducers of the distinct activation profile warrant further investigation.

  13. Neuronal differentiation modulates the dystrophin Dp71d binding to the nuclear matrix

    International Nuclear Information System (INIS)

    Rodriguez-Munoz, Rafael; Villarreal-Silva, Marcela; Gonzalez-Ramirez, Ricardo; Garcia-Sierra, Francisco; Mondragon, Monica; Mondragon, Ricardo; Cerna, Joel; Cisneros, Bulmaro

    2008-01-01

    The function of dystrophin Dp71 in neuronal cells remains unknown. To approach this issue, we have selected the PC12 neuronal cell line. These cells express both a Dp71f cytoplasmic variant and a Dp71d nuclear isoform. In this study, we demonstrated by electron and confocal microscopy analyses of in situ nuclear matrices and Western blotting evaluation of cell extracts that Dp71d associates with the nuclear matrix. Interestingly, this binding is modulated during NGF-induced neuronal differentiation of PC12 cells with a twofold increment in the differentiated cells, compared to control cells. Also, distribution of Dp71d along the periphery of the nuclear matrix observed in the undifferentiated cells is replaced by intense fluorescent foci localized in Center of the nucleoskeletal structure. In summary, we revealed that Dp71d is a dynamic component of nuclear matrix that might participate in the nuclear modeling occurring during neuronal differentiation

  14. Cytotoxic T lymphocyte responses by chimeric thymocytes. Self-recognition is determined early in T cell development

    International Nuclear Information System (INIS)

    Kruisbeek, A.M.; Hodes, R.J.; Singer, A.

    1981-01-01

    In this study the cytotoxic T lymphocyte (CTL) recognition pattern of thymocytes from recently reconstituted parent leads to F1 and F1 leads to parent radiation bone marrow chimeras was investigated. Chimeric thymocytes were entirely of donor origin approximately 4 weeks after irradiation and reconstitution but were not capable of autonomously generating either alloreactive or trinitrophenyl (TNP)-modified-self-reactive CTL responses. These experiments demonstrte that even at the earliest time CTL effectors of donor origin from the thymuses of chimeras can be studied, their self-receptor repertoire has already been restricted to recognition of host MHC determinants. These results support the cocept that the host environment influences the self-recognition capacity of T cells at the pre- or intrathymic stage of differentation

  15. Dynamic Positioning of Ships : A nonlinear control design study

    NARCIS (Netherlands)

    Muhammad, S.

    2012-01-01

    Dynamic positioning (DP) is relatively a new technique used to maintain the position and heading of ships in various offshore operations. Due to the features like better safety and operating efficiency, DP systems are becoming more and more popular. This thesis mainly focusses on the control system

  16. Transgenic expression of microRNA-181d augments the stress-sensitivity of CD4(+CD8(+ thymocytes.

    Directory of Open Access Journals (Sweden)

    Serkan Belkaya

    Full Text Available Physiological stress resulting from infections, trauma, surgery, alcoholism, malnutrition, and/or pregnancy results in a substantial depletion of immature CD4(+CD8(+ thymocytes. We previously identified 18 distinct stress-responsive microRNAs (miRs in the thymus upon systemic stress induced by lipopolysaccharide (LPS or the synthetic glucocorticoid, dexamethasone (Dex. MiRs are short, non-coding RNAs that play critical roles in the immune system by targeting diverse mRNAs, suggesting that their modulation in the thymus in response to stress could impact thymopoiesis. MiR-181d is one such stress-responsive miR, exhibiting a 15-fold down-regulation in expression. We utilized both transgenic and gene-targeting approaches to study the impact of miR-181d on thymopoiesis under normal and stress conditions. The over-expression of miR-181d in developing thymocytes reduced the total number of immature CD4(+CD8(+ thymocytes. LPS or Dex injections caused a 4-fold greater loss of these cells when compared with the wild type controls. A knockout mouse was developed to selectively eliminate miR-181d, leaving the closely spaced and contiguous family member miR-181c intact. The targeted elimination of just miR-181d resulted in a thymus stress-responsiveness similar to wild-type mice. These experiments suggest that one or more of three other miR-181 family members have overlapping or compensatory functions. Gene expression comparisons of thymocytes from the wild type versus transgenic mice indicated that miR-181d targets a number of stress, metabolic, and signaling pathways. These findings demonstrate that selected miRs enhance stress-mediated thymic involution in vivo.

  17. In vitro analysis of age-related changes in the developmental potential of bone marrow thymocyte progenitors.

    Science.gov (United States)

    Sharp, A; Kukulansky, T; Globerson, A

    1990-12-01

    Mechanisms underlying the age-related decrease in the developmental capacity of thymocyte progenitors from the bone marrow (BM) were analyzed, focussing on interaction of these cells with the thymic microenvironment. We employed the experimental model in which mixtures of young and old mouse BM cells, congenic for the Thy-1 marker, were seeded onto fetal thymus (FT) explains depleted of self lymphocytes and the levels of Thy-1+ cells developing from each of the two donor types were measured. When cells from young and old BM donors were seeded simultaneously, in saturating quantities, a higher level of T cells developed from the young donors. To find out whether there were originally more thymocyte progenitors in the young BM, we carried out the competitive colonization under limiting dilution conditions and found that the advantage of the young had diminished under these conditions, thus suggesting that the age-related changes could not be related solely to quantitative differences. We then incubated the FT sequentially with old donor cells for 24 h, followed by young for an additional 48 h and found that the advantage of the young progenitors was eliminated. We thus established that the initial stage of colonization of the FT was important in determining the outcome of the subsequent development. The kinetics of simultaneous competition within the FT, however, revealed that the advantage of the young BM-derived cells became significant only from day 7 in organ culture, thus suggesting that sequential divisions of these cells were at a higher level than those of the old. Recolonization of FT explants by young or old BM-derived thymocytes obtained from the first colonization of the FT stroma showed a reduced, but still significant advantage for the young BM-derived cells over the old. Thus, we concluded that the old BM thymocyte progenitors manifested a qualitative disadvantage which became apparent during competitive colonization of the FT.

  18. Molecular dynamics study of lipid bilayers modeling the plasma membranes of normal murine thymocytes and leukemic GRSL cells.

    Science.gov (United States)

    Andoh, Yoshimichi; Okazaki, Susumu; Ueoka, Ryuichi

    2013-04-01

    Molecular dynamics (MD) calculations for the plasma membranes of normal murine thymocytes and thymus-derived leukemic GRSL cells in water have been performed under physiological isothermal-isobaric conditions (310.15K and 1 atm) to investigate changes in membrane properties induced by canceration. The model membranes used in our calculations for normal and leukemic thymocytes comprised 23 and 25 kinds of lipids, respectively, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, sphingomyelin, lysophospholipids, and cholesterol. The mole fractions of the lipids adopted here were based on previously published experimental values. Our calculations clearly showed that the membrane area was increased in leukemic cells, and that the isothermal area compressibility of the leukemic plasma membranes was double that of normal cells. The calculated membranes of leukemic cells were thus considerably bulkier and softer in the lateral direction compared with those of normal cells. The tilt angle of the cholesterol and the conformation of the phospholipid fatty acid tails both showed a lower level of order in leukemic cell membranes compared with normal cell membranes. The lateral radial distribution function of the lipids also showed a more disordered structure in leukemic cell membranes than in normal cell membranes. These observations all show that, for the present thymocytes, the lateral structure of the membrane is considerably disordered by canceration. Furthermore, the calculated lateral self-diffusion coefficient of the lipid molecules in leukemic cell membranes was almost double that in normal cell membranes. The calculated rotational and wobbling autocorrelation functions also indicated that the molecular motion of the lipids was enhanced in leukemic cell membranes. Thus, here we have demonstrated that the membranes of thymocyte leukemic cells are more disordered and more fluid than normal cell membranes. Copyright © 2013

  19. Restriction fragment polymorphism (RFLP) of a "new" HLA-DP specificity, CDP-HEI

    DEFF Research Database (Denmark)

    Hyldig-Nielsen, J J; Ødum, Niels; Morling, Niels

    1988-01-01

    Southern blotting with a DP beta cDNA probe of MspI digested DNA from 83 healthy unrelated individuals revealed a 1.8 kb fragment present in all four individuals (and no others) possessing the newly determined DP specificity, CDP-HEI.......Southern blotting with a DP beta cDNA probe of MspI digested DNA from 83 healthy unrelated individuals revealed a 1.8 kb fragment present in all four individuals (and no others) possessing the newly determined DP specificity, CDP-HEI....

  20. DP 71 AND BETA DYSTROGLYCAN INTERACTION: A MOLECULAR MODELING APPROACH TO UNDERSTAND DUCHENNE MUSCULAR DYSTROPHY

    Directory of Open Access Journals (Sweden)

    Simanti Bhattacharya,

    2013-12-01

    Full Text Available Dp 71 is the most prevalent and widely expressed non muscle isoform of dystrophin (Dp and its mutations are associated with Duchenne muscular dystrophy, a severe form of muscular disorder. Dp 71 deviates from the canonical Dp by means of its truncated N terminal which also has abolished certain amino acids that comprise WW domain in the canonical form. This WW domain is very crucial for Dp’s interaction with partner proteins to establish a bridge between extra cellular matrices and cellular cytoskeleton. In our current study we have employed molecular modeling technique to understand the structural architecture of the N terminal region of Dp 71 and its deviation from the canonical form. We have further extended our studies to analyze the interaction probabilities between Dp 71 and β-DG applying molecular docking. Our studies for the first time have revealed that in spite of the underlying differences in terms of amino acids and structural organization, Dp 71 can interact with β-DG with its N terminal region which shares the similar molecular surface with the canonical form of Dp. These findings have opened up a platform to investigate the molecular interactions, spatio temporal orientations of the amino acids of Dp 71 and β-DG to understand the onset of DMD in much more greater detail

  1. PROFIBUS-DP to G-64 configurable interface

    CERN Document Server

    Carlier, E; Rochez, J; Serrano, J; CERN. Geneva. SPS and LHC Division

    2001-01-01

    Since many years, the SL/BT group has developed specific G-64 hardware modules for the control of the fast pulsed magnets. Up to now, the integration of these modules into the accelerator control system has been done using a MIL1553 fieldbus in command/response mode connected to a LynxOS front-end. In 1996 and 1998, recommendations for the use of industrial fieldbuses and programmable logic controllers were issued at CERN. In order to integrate the existing G-64 hardware into this environment based on industrial components, an interface between the G-64 bus and the PROFIBUS-DP fieldbus has been developed. This interface is based on a modular and open hardware architecture with on one side an off-the-shelf industrial, fieldbus independent, interface and on the other side a FPGA (Field Programmable Gate Array). Required functionality has been implemented within the FPGA with VHDL (Very high-speed integrated circuit Hardware Description Language).

  2. A valoração de traços de concordância dentro do DP Concord features valuing within DP

    Directory of Open Access Journals (Sweden)

    Telma M.V. Magalhães

    2004-06-01

    Full Text Available Este trabalho argumenta em favor da valoração dos traços de concordância dentro do DP em termos da operação Agree (Chomsky, 1999 sem a necessidade de estipular nenhum outro mecanismo para tanto. Mostro que Agree dá conta da valoração de traços tanto no nível da sentença quanto no nível do DP, contra a sugestão de Chomsky (1999 de que concordância no DP envolveria algum outro mecanismo de checagem.This paper argues in favor of a concord features valuing within the DP in terms of the Agree operation (Chomsky, 1999, with no recourse to any other mechanism. I show that Agree accounts for feature valuing both in the sentence level as well as in the DP, contrary to Chomsky's (1999 suggestion that concord in DP should involve some other checking mechanism.

  3. Effect of gamma-radiation on direct intercellular interaction (rosette formation) between thymus macrophages and thymocytes

    International Nuclear Information System (INIS)

    Belyakov, I.M.; Yarilin, A.A.

    1992-01-01

    In experiments with mice, the effect of radiation on resette formation between thymus macrophages (Th-MPh) and thymocytes (Thc) was studied on days 1, 4, 12, 30, and 60 following gamma-irradiation with doses of 0.5, 2, 4, and 8 Gy. gamma-Irradiation with doses of above 2 Gy was shown to cause a dose-dependent inhibition of rosette formation of Th-MPh with Thc in vitro. Two types of rosette-forming Th-MPh were identified: RFMPhII with low rate of binding to Thc and RFMPhII with high rate of binding to Thc. Radiation affects mainly the RFMPhII content. The total population of rosette-forming Th-MPh was restored on day 60 mainly due to cells with low rate of rosette formation. The EC supernatant promoted rosette formation of exposed Th-MPh with Thc. The effect was maximum at early times following irradiation of Th-MPh with a dose of 4 Gy

  4. Inhibiting the repair of DNA damage induced by gamma irradiation in rat thymocytes

    International Nuclear Information System (INIS)

    Smit, J.A.; Stark, J.H.

    1994-01-01

    This study assessed the ability of 11 established and potential radiosensitizing agents to retard the repair of radiation-induced DNA damage with a view to enhancing the immunosuppressive effects of in vivo lymphoid irradiation. The capability of irradiated rat thymocytes to repair DNA damage was assessed by an adaptation of the fluorimetric unwinding method. Three compounds, 3-aminobenzamide (3-AB), novobiocin and flavone-8-acetic acid (FAA), inhibited repair significantly. We also report the effect of low-dose irradiation combined with repair inhibitors on the relationship between DNA strand breaks, fragmentation, cell viability and use of nicotinamide adenine dinucleotide (NAD). DNA fragmentation was increased by 1 mM/l FAA, 1 mM/l novobiocin and 50 μM/l RS-61443 within 3 h of incubation. The latter two compounds also proved cytotoxic. All three drugs augmented the effect of ionizing radiation on the use of NAD. Of the agents investigated, FAA showed the most promise for augmenting the immunosuppressive action of irradiation at nontoxic, pharmacokinetically achievable concentrations. 33 refs., 1 fig., 2 tabs

  5. Immune reconstitution with two different rabbit polyclonal anti-thymocytes globulins.

    Science.gov (United States)

    Bamoulid, Jamal; Crepin, Thomas; Gaiffe, Emilie; Laheurte, Caroline; Moulin, Bruno; Frimat, Luc; Rieu, Philippe; Mousson, Christiane; Durrbach, Antoine; Heng, Anne-Elisabeth; Rebibou, Jean-Michel; Saas, Philippe; Courivaud, Cécile; Ducloux, Didier

    2017-12-01

    Broad T cell depletion by polyclonal anti-thymocyte globulins (ATG) has been used for many years as a part of immunosuppressive treatment in transplantation. Currently, two different ATG are used in clinical practice, Thymoglobulin and Grafalon. Due to differences in the immunization source, these products contain different specificities and quantity of antibodies. These differences may have clinical consequences. We conducted a nested study in a large prospective multicentric cohort of kidney transplant to determine whether Grafalon-treated and Thymoglobulin-treated patients experience different lymphocyte reconstitution and clinical outcomes. 182 patients matched for age, gender, CMV status, CMV prophylaxis, number of previous transplantation, and maintenance immunosuppressive treatment were included (Thymoglobulin, [n=91]; Grafalon®, [n=91]). One-year post-transplant, recent thymic emigrants were significantly decreased (12±10% vs 21±12%; p<0.001) in Grafalon-treated patients. By contrast, T cell activation (CD38+DR+Ki67+) and senescence (CD8+CD57+CD28-) was increased in Thymoglobulin-treated patients. Compared to Grafalon, Thymoglobulin was not associated with a significantly different rate of acute rejection. CMV disease (p=0.013) was more frequent in Thymoglobulin-treated patients. Grafalon and Thymoglobulin seem to be equivalent to prevent acute rejection. CMV disease is more frequent in Thymoglobulin-treated patients. One year post-transplant immune profile profoundly differs according to the type of ATG. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Suppressive effects of diltiazem and verapamil on delayed rectifier K(+)-channel currents in murine thymocytes.

    Science.gov (United States)

    Baba, Asuka; Tachi, Masahiro; Maruyama, Yoshio; Kazama, Itsuro

    2015-10-01

    Lymphocytes predominantly express delayed rectifier K(+)-channels (Kv1.3) in their plasma membranes, and these channels play crucial roles in the lymphocyte activation and proliferation. Since diltiazem and verapamil, which are highly lipophilic Ca(2+) channel blockers (CCBs), exert relatively stronger immunomodulatory effects than the other types of CCBs, they would affect the Kv1.3-channel currents in lymphocytes. Employing the standard patch-clamp whole-cell recording technique in murine thymocytes, we examined the effects of these drugs on the channel currents and the membrane capacitance. Both diltiazem and verapamil significantly suppressed the peak and the pulse-end currents of the channels, although the effects of verapamil were more marked than those of diltiazem. Both drugs significantly lowered the membrane capacitance, indicating the interactions between the drugs and the plasma membranes. This study demonstrated for the first time that CCBs, such as diltiazem and verapamil, exert inhibitory effects on Kv1.3-channels expressed in lymphocytes. The effects of these drugs may be associated with the mechanisms of immunomodulation by which they decrease the production of inflammatory cytokines. Copyright © 2015 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  7. Human TFDP3, a novel DP protein, inhibits DNA binding and transactivation by E2F

    DEFF Research Database (Denmark)

    Qiao, Huan; Di Stefano, Luisa; Tian, Chan

    2006-01-01

    The two known DP proteins, TFDP1 and -2, bind E2Fs to form heterodimers essential for high affinity DNA binding and efficient transcriptional activation/repression. Here we report the identification of a new member of the DP family, human TFDP3. Despite the high degree of sequence similarity, TFD...

  8. HLA-DP antigens are involved in the susceptibility to multiple sclerosis

    DEFF Research Database (Denmark)

    Ødum, Niels; Hyldig-Nielsen, J J; Morling, N

    1988-01-01

    Forty-five unrelated patients with multiple sclerosis (MS) from Sweden and 166 Danish controls were typed for HLA-DP using Primed Lymphocyte Typing. Thirty-nine MS-patients and 63 controls were also DNA-typed with the Restriction Fragment Length Polymorphism (RFLP) technique for HLA-DP and -DR ge...... susceptibility to MS....

  9. HLA-DP related suppression of mixed lymphocyte reaction with alloactivated lymphocytes

    DEFF Research Database (Denmark)

    Ødum, Niels; Hofmann, B; Jakobsen, B K

    1986-01-01

    We studied the influence of HLA class I and class II antigens on the suppression of the MLR induced by primed lymphocytes (PLs) alloactivated in vitro. The suppression of 14 different PLs of 83 MLRs was analyzed. The PLs were primed against (i) HLA-DP (SB) (ii) HLA-DR/DQ or (iii) both HLA-DP and ...

  10. Restriction fragment polymorphism (RFLP) of a "new" HLA-DP specificity, CDP-HEI

    DEFF Research Database (Denmark)

    Hyldig-Nielsen, J J; Ødum, Niels; Morling, Niels

    1988-01-01

    Southern blotting with a DP beta cDNA probe of MspI digested DNA from 83 healthy unrelated individuals revealed a 1.8 kb fragment present in all four individuals (and no others) possessing the newly determined DP specificity, CDP-HEI....

  11. On key factors influencing ductile fractures of dual phase (DP) steels

    International Nuclear Information System (INIS)

    Sun, X.; Choi, K.S.; Soulami, A.; Liu, W.N.; Khaleel, M.A.

    2009-01-01

    In this paper, we examine the key factors influencing ductile failure of various grades of dual phase (DP) steels using the microstructure-based modeling approach. Various microstructure-based finite element models are generated based on the actual microstructures of DP steels with different martensite volume fractions. These models are, then, used to investigate the influence of ductility of the constituent ferrite phase and also the influence of voids introduced in the ferrite phase on the overall ductility of DP steels. It is found that with volume fraction of martensite in the microstructure less than 15%, the overall ductility of the DP steels strongly depends on the ductility of the ferrite matrix, hence pre-existing micro-voids in the microstructure significantly reduce the overall ductility of the steel. When the volume fraction of martensite is above 15%, the pre-existing voids in the ferrite matrix does not significantly reduce the overall ductility of the DP steels, and the overall ductility is more influenced by the mechanical property disparity between the two phases. The applicability of the phase inhomogeneity driven ductile failure of DP steels is then discussed based on the obtained computational results for various grades of DP steels, and the experimentally obtained scanning electron microscopy (SEM) pictures of the corresponding grades of DP steels near fracture surface are used as evidence for result validations.

  12. HLA-DP related suppression of mixed lymphocyte reaction with alloactivated lymphocytes

    DEFF Research Database (Denmark)

    Ødum, Niels; Hofmann, B; Jakobsen, B K

    1986-01-01

    We studied the influence of HLA class I and class II antigens on the suppression of the MLR induced by primed lymphocytes (PLs) alloactivated in vitro. The suppression of 14 different PLs of 83 MLRs was analyzed. The PLs were primed against (i) HLA-DP (SB) (ii) HLA-DR/DQ or (iii) both HLA-DP and DR...

  13. Latitudinal profile of the ionospheric disturbance dynamo magnetic signature: comparison with the DP2 magnetic disturbance

    Directory of Open Access Journals (Sweden)

    K. Z. Zaka

    2009-09-01

    Full Text Available During magnetic storms, the auroral electrojets intensification affects the thermospheric circulation on a global scale. This process which leads to electric field and current disturbance at middle and low latitudes, on the quiet day after the end of a storm, has been attributed to the ionospheric disturbance dynamo (Ddyn. The magnetic field disturbance observed as a result of this process is the reduction of the H component amplitude in the equatorial region which constitutes the main characteristic of the ionospheric disturbance dynamo process, associated with a westward electric current flow. The latitudinal profile of the Ddyn disturbance dynamo magnetic signature exhibits an eastward current at mid latitudes and a westward one at low latitudes with a substantial amplification at the magnetic equator. Such current flow reveals an "anti-Sq" system established between the mid latitudes and the equatorial region and opposes the normal Sq current vortex. However, the localization of the eastward current and consequently the position and the extent of the "anti-Sq" current vortex changes from one storm to another. Indeed, for a strong magnetic storm, the eastward current is well established at mid latitudes about 45° N and for a weak magnetic storm, the eastward current is established toward the high latitudes (about 60° N, near the Joule heating region, resulting in a large "anti-Sq" current cell. The latitudinal profile of the Ddyn disturbance as well as the magnetic disturbance DP2 generated by the mechanism of prompt penetration of the magnetospheric convection electric field in general, show a weak disturbance at the low latitudes with a substantial amplification at the magnetic equator. Due to the intensity of the storm, the magnitude of the DP2 appears higher than the Ddyn over the American and Asian sector contrary to the African sector.

  14. Creativity in context; the courage in Therivel's GAM/DP.

    Science.gov (United States)

    Gruber, Craig W

    2013-03-01

    The desire to quantify and categorize creativity so that we can easily identify the individual characteristics which serve as its precursors is one which authors have been attempting to successfully complete for some time. In this light, William Therivel's High Creativity Unmasked, discusses how his GAM/DP theory accounts for highly creative people from history as well as a rationale for the rise and fall of creativity in selected cultures throughout history. By examining the latest published work on how Genetic Endowment, Assistances, Misfortunes and Divisions of Power, and how they relate to creativity, Therivel proposes unique rationales for the rises and declines of cultures. This paper examines how creativity and courage work together and in some cases side by side, to explain more fully the role of creativity in culture. This article examines some of Therivel's examples and provides alternate explanations for the phenomena he ascribes to the decline of creativity. In addition, the complexity of both creativity and courage are discussed, both in the current context, and in the next steps for research and theoretical discussion.

  15. Cell proliferation and thymocyte subset reconstitution in sublethally irradiated mice: Compared kinetics of endogenous and intrathymically transferred progenitors

    International Nuclear Information System (INIS)

    Penit, C.; Ezine, S.

    1989-01-01

    After sublethal (6 Gy) whole-body irradiation, the C57BL/Ba (Thy-1.1) murine thymus regenerated in two waves, on days 3-10 and 25-32, separated by a severe relapse. The second phase of depletion-reconstitution reproduced the first one, in a less synchronous manner. The depletion affected all cell subsets, but CD4+ CD8- cells decreased later than immature cells. Cell proliferation, measured by BrdUrd incorporation, started on day 3 after irradiation and concerned CD4- CD8-, CD4- CD8+, and CD4+ CD8+ cells, sequentially. CD4+ CD8- cells never represented a significant percentage of cycling cells. When irradiation was immediately followed by an intrathymic injection of 10(5) C57BL/Ka (Thy-1.2) bone marrow cells, the relapse in thymus reconstitution was no longer observed. Detected with anti-Thy-1.2 antibodies, donor cells started cycling on day 14 and showed only one wave of proliferation. In these chimeras, recipient thymocytes behave exactly like thymocytes of solely irradiated mice. Intrathymically transferred CD4- CD8- thymocytes 10(5) showed the same proliferation kinetics as endogenous cells, with a peak in number on day 10 but completely disappeared from the thymus on days 14-21. These data reflect maturational differences between intrathymic and bone marrow precursor cells and suggest different radiosensitivities not linked to proliferative status. The resting state of the thymus immigrants was shown by the absence of Thy-1 acquisition by bone marrow cells continuously labeled for 10 days with BrdUrd in vivo before intrathymic transfer. When such labeled bone marrow cells were injected in the thymus, only the minor BrdUrd- subset gave rise to Thy-1+ cells

  16. Innate-like CD4 T cells selected by thymocytes suppress adaptive immune responses against bacterial infections

    OpenAIRE

    Qiao, Yu; Gray, Brian M.; Sofi, Mohammed H.; Bauler, Laura D.; Eaton, Kathryn A.; O'Riordan, Mary X. D.; Chang, Cheong-Hee

    2011-01-01

    We have reported a new innate-like CD4 T cell population that expresses cell surface makers of effector/memory cells and produce Th1 and Th2 cytokines immediately upon activation. Unlike conventional CD4 T cells that are selected by thymic epithelial cells, these CD4 T cells, named T-CD4 T cells, are selected by MHC class II expressing thymocytes. Previously, we showed that the presence of T-CD4 T cells protected mice from airway inflammation suggesting an immune regulatory role of T-CD4 T ce...

  17. Diethylstilbestrol alters positive and negative selection of T cells in the thymus and modulates T-cell repertoire in the periphery

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Nicole [Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298 (United States); Nagarkatti, Mitzi [Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298 (United States); Nagarkatti, Prakash S [Department of Pharmacology and Toxicology, PO Box 980613, Virginia Commonwealth University Medical Center, Richmond, VA 23298-0613 (United States)

    2006-04-15

    Prenatal exposure to diethylstilbestrol (DES) is known to cause altered immune functions and increased susceptibility to autoimmune disease in humans. In the current study, we investigated the effects of DES on T-cell differentiation in the thymus using the HY-TCR transgenic (Tg) mouse model in which the female mice exhibit positive selection of T cells bearing the Tg TCR, while the male mice show negative selection of such T cells. In female HY-TCR-Tg mice, exposure to DES showed more pronounced decrease in thymic cellularity when compared to male mice. Additionally, female mice also showed a significant decrease in the proportion of double-positive (DP) T cells in the thymus and HY-TCR-specific CD8{sup +} T cells in the periphery. Male mice exhibiting negative selection also showed decreased thymic cellularity following DES exposure. Moreover, the male mice showed increased proportion of double-negative (DN) T cells in the thymus and decreased proportion of CD8{sup +} T cells. The density of expression of HY-TCR on CD8{sup +} cells was increased following DES exposure in both females and males. Finally, the proliferative response of thymocytes to mitogens and peripheral lymph node T cells to male H-Y antigen was significantly altered in female and male mice following DES treatment. Taken together, these data suggest that DES alters T-cell differentiation in the thymus by interfering with positive and negative selection processes, which in turn modulates the T-cell repertoire in the periphery.

  18. Early double-negative thymocyte export in Trypanosoma cruzi infection is restricted by sphingosine receptors and associated with human chagas disease.

    Directory of Open Access Journals (Sweden)

    Ailin Lepletier

    2014-10-01

    Full Text Available The protozoan parasite Trypanosoma cruzi is able to target the thymus and induce alterations of the thymic microenvironmental and lymphoid compartments. Acute infection results in severe atrophy of the organ and early release of immature thymocytes into the periphery. To date, the pathophysiological effects of thymic changes promoted by parasite-inducing premature release of thymocytes to the periphery has remained elusive. Herein, we show that sphingosine-1-phosphate (S1P, a potent mediator of T cell chemotaxis, plays a role in the exit of immature double-negative thymocytes in experimental Chagas disease. In thymuses from T. cruzi-infected mice we detected reduced transcription of the S1P kinase 1 and 2 genes related to S1P biosynthesis, together with increased transcription of the SGPL1 sphingosine-1-lyase gene, whose product inactivates S1P. These changes were associated with reduced intrathymic levels of S1P kinase activity. Interestingly, double-negative thymocytes from infected animals expressed high levels of the S1P receptor during infection, and migrated to lower levels of S1P. Moreover, during T. cruzi infection, this thymocyte subset expresses high levels of IL-17 and TNF-α cytokines upon polyclonal stimulation. In vivo treatment with the S1P receptor antagonist FTY720 resulted in recovery the numbers of double-negative thymocytes in infected thymuses to physiological levels. Finally, we showed increased numbers of double-negative T cells in the peripheral blood in severe cardiac forms of human Chagas disease.

  19. The role of the prostaglandin D2 receptor, DP, in eosinophil trafficking

    DEFF Research Database (Denmark)

    Schratl, Petra; Royer, Julia F; Kostenis, Evi

    2007-01-01

    of DP has remained unclear. We report in this study that, in addition to CRTH2, the DP receptor plays an important role in eosinophil trafficking. First, we investigated the release of eosinophils from bone marrow using the in situ perfused guinea pig hind limb preparation. PGD2 induced the rapid......Prostaglandin (PG) D2 is a major mast cell product that acts via two receptors, the D-type prostanoid (DP) and the chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) receptors. Whereas CRTH2 mediates the chemotaxis of eosinophils, basophils, and Th2 lymphocytes, the role...

  20. Typing for HLA-D/DR associated DP-antigens with the primed lymphocyte typing (PLT) technique

    DEFF Research Database (Denmark)

    Morling, N; Jakobsen, B K; Platz, P

    1980-01-01

    assignments, which does not agree with other studies. The DP-antigen frequencies among the controls were calculated and the estimated sum of gene frequency corresponding to definable DP-antigens was 0.94 indicating that about 12% of random individuals possess as yet undefined DP-antigens....

  1. Thymic Nurse Cells Participate in Heterotypic Internalization and Repertoire Selection of Immature Thymocytes; Their Removal from the Thymus of Autoimmune Animals May be Important to Disease Etiology.

    Science.gov (United States)

    Guyden, J C; Martinez, M; Chilukuri, R V E; Reid, V; Kelly, F; Samms, M-O D

    2015-01-01

    Thymic nurse cells (TNCs) are specialized epithelial cells that reside in the thymic cortex. The initial report of their discovery in 1980 showed TNCs to contain up to 200 thymocytes within specialized vacuoles in their cytoplasm. Much has been reported since that time to determine the function of this heterotypic internalization event that exists between TNCs and developing thymocytes. In this review, we discuss the literature reported that describes the internalization event and the role TNCs play during T cell development in the thymus as well as why these multicellular complexes may be important in inhibiting the development of autoimmune diseases.

  2. Dechlorane Plus (DP) in air and plants at an electronic waste (e-waste) site in South China

    Energy Technology Data Exchange (ETDEWEB)

    Chen Shejun [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); Tian Mi; Wang Jing; Shi Tian [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); Graduate School, Chinese Academy of Sciences, Beijing 100049 (China); Luo Yong [Guangdong Forestry Survey and Planning Institute, Guangzhou 510520 (China); Luo Xiaojun [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); Mai Bixian, E-mail: nancymai@gig.ac.cn [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China)

    2011-05-15

    Air and foliage samples (Eucalyptus spp. and Pinus massoniana Lamb.) were collected from e-waste and reference sites in South China and analyzed for Dechlorane Plus (DP) and two dechlorinated DPs. DP concentrations in the air were 13.1-1794 pg/m{sup 3} for the e-waste site and 0.47-35.7 pg/m{sup 3} for the reference site, suggesting the recycling of e-waste is an important source of DP to the environment. Plant DP, with concentrations of 0.45-51.9 ng/g dry weight at the e-waste site and 0.09-2.46 ng/g at the reference site, exhibited temporal patterns similar to the air DP except for pine needle at the reference site. The air-plant exchange of DP could be described with the two-compartment model. Anti-Cl{sub 11} DP was measured in most air and plant samples from the e-waste site. The ratios of anti-Cl{sub 11} DP to anti-DP in the air and plants may indicate the preferential uptake of dechlorinated DP by plant compared with DP. - Highlights: > Dechlorane Plus was widely present in the air and plants in South China. > Temporal patterns of the plant DP could be described with the two-compartment model. > Plant uptake can efficiently reduce air DP concentration at the reference site. > Anti-Cl{sub 11} DP was measured in most air and plant samples from the e-waste site. - E-waste recycling in South China results in wide occurrence of DP in the air and plant.

  3. Association of HLA-DP/DQ and STAT4 polymorphisms with HBV infection outcomes and a mini meta-analysis.

    Science.gov (United States)

    Liao, Yun; Cai, Bei; Li, Yi; Chen, Jie; Tao, Chuanmin; Huang, Hengjian; Wang, Lanlan

    2014-01-01

    Though HLA-DP/DQ is regarded to associate with HBV susceptibility and HBV natural clearance, its role in hepatocellular carcinoma (HCC) development is obscure. And the role of STAT4 in HBV susceptibility and clearance as well as HCC development is still contentious. Therefore, we conducted this study, aiming to clarify these obscure relationships. We recruited 1312 Chinese Han subjects including healthy controls, HBV carriers and HCC patients in the experiment stage. The meta-analysis included 3467 HCC patients and 5821 HBV carriers to appraise the association with HCC development. Consistent with previous studies, HLA-DP/DQ associated with HBV susceptibility and HBV natural clearance (prs7574865, we did not find any significant association with HBV susceptibility (OR = 0.91, 95%CI = 0.66-1.26) or HBV natural clearance (OR = 1.13, 95%CI = 0.86-1.49). Moreover, current data failed to acquire positive connection of rs7574865 with HCC development (experiment, OR = 0.86, 95%CI = 0.62-1.19; meta-analysis, OR = 0.87, 95%CI = 0.74-1.03), which may be due to the small sample size. HLA-DP/DQ polymorphisms (rs3077, rs9277535, rs7453920) did not associate with HCC development, but did correlate with HBV susceptibility and HBV natural clearance. STAT4 rs7574865 seemed not to correlate with HBV susceptibility or natural clearance. And it seemed rather ambiguous in its role on HCC development at present.

  4. Coherent production features in dp interactions at 11.9 GeV/c

    International Nuclear Information System (INIS)

    Braun, H.; Brick, D.; Fridman, A.; Gerber, J.P.; Juillot, P.; Maurer, G.; Alexander, G.; Dagan, S.; Grunhaus, J.; Levy, A.; Lissauer, D.; Oren, Y.

    1975-01-01

    The two pion production in coherent dp reactions is studied. The resonance production as well as the d* effect and the fragmentation processes are discussed. The present reults are compared with available coherent anti-p d data [fr

  5. Sequential analysis of the virus-immune responder characteristics of thymocytes from F1→parent radiation chimeras

    International Nuclear Information System (INIS)

    Korngold, R.; Doherty, P.C.

    1982-01-01

    The virus-immune responder characteristics of thymocytes, spleen and lymph node (LN) cells from (P 1 x P 2 )F 1 →P 1 radiation chimeras have been examined sequentially at weekly intervals. Adoptively-transferred thymocytes generate strong cytotoxic thymus-derived lymphocyte (CTL) responses from 28 to 100 days after reconstitution with bone marrow, which are almost invariably restricted to recognition of virus presented in the context of P 1 . This pattern of H-2 restriction is also maintained for spleen and LN cells from the [(H-2sup(kxd)F 1 →H-2sup(k)] and [(H-2sup(kxb)F 1 →H-2sup(k)] combinations but there is random emergence of reactivity to H-2sup(k)+virus for peripheral lymphoid cells from [(H-2sup(kxb)F 1 →H-2sup(b)] chimeras. Treatment of established [(P 1 xP 2 )]F 1 →P 1 ] chimeras with a low dose of cyclophosphamide (Cy) did not lead to the emergence of significant CTL effector function for P2 + virus. Also, administration of a large dose of Cy prior to irradiation of the chimera recipients did not modify the H-2 restriction profile of the chimera, though the level of CTL responsiveness associated with the appropriate H-2 type was apparently enhanced. (Auth.)

  6. Semiconservative and unscheduled DNA-synthesis of rat thymocytes under the influence of some radioprotecting and radiosensitizing agents

    International Nuclear Information System (INIS)

    Tempel, K.; Wulffius-Kock, M.; Winkle, J.; Schmerold, I.

    1982-01-01

    The effects of aminoethylisothiuroniumbromide (AET), cysteamine (CY-A), cysteine (CY-E), glutathione (GLU), mercaptoethanol (MA), mercaptopropionylglycine (MPG), N-ethylmaleimide (NEM), metronidazole (MNA), nitroacetophenone (NAP), nitrofurazone (NFA), arabinofuranosylcytosine (araC), fluorouracil (FU), adriamycin (AM), ethidiumbromide (E), bleomycin (BM), and diethyldithiocarbamate (DDC) on the semiconservative and unscheduled incorporation of 3 H-thymidine into the DNA were tested on rat thymocytes in vitro. DNA damage has been measured using the hydroxylapatite system. Unscheduled DNA synthesis was induced by UV-light and/or X-irradiation. The semiconservative DNA synthesis was inhibited by the above subtrances-with exception of MA and MPG. Aminothioles, NAP, NFA, and BM enhanced, araC, FU, AM, E, and DDC diminished unscheduled DNA synthesis. After alkaline unwinding, the duplex form of DNA decreased under the influence of CY-A, CY-E, GLU, MPG, NEM, NAP, NFA, araC, FU, AM, E, and BM. It is suggested that stimulation of unscheduled DNA synthesis combined with a transient decrease of semiconservative DNA synthesis will amplify the DNA repair capacity of thymocytes, whereas radiation damage may be intensified by araC, FU, AM,E, and DDC - at least partly, through inhibition of unscheduled DNA synthesis. With respect to the action of NAP, NFA, and BM, DNA repair may be concerned in a more indirect manner. (orig.) [de

  7. Semiconservative and unscheduled DNA-synthesis of rat thymocytes under the influence of some radioprotecting and radiosensitizing agents

    Energy Technology Data Exchange (ETDEWEB)

    Tempel, K.; Wulffius-Kock, M.; Winkle, J.; Schmerold, I.

    1982-02-01

    The effects of aminoethylisothiuroniumbromide (AET), cysteamine (CY-A), cysteine (CY-E), glutathione (GLU), mercaptoethanol (MA), mercaptopropionylglycine (MPG), N-ethylmaleimide (NEM), metronidazole (MNA), nitroacetophenone (NAP), nitrofurazone (NFA), arabinofuranosylcytosine (araC), fluorouracil (FU), adriamycin (AM), ethidiumbromide (E), bleomycin (BM), and diethyldithiocarbamate (DDC) on the semiconservative and unscheduled incorporation of /sup 3/H-thymidine into the DNA were tested on rat thymocytes in vitro. DNA damage has been measured using the hydroxylapatite system. Unscheduled DNA synthesis was induced by UV-light and/or X-irradiation. The semiconservative DNA synthesis was inhibited by the above substances-with exception of MA and MPG. Aminothioles, NAP, NFA, and BM enhanced, araC, FU, AM, E, and DDC diminished unscheduled DNA synthesis. After alkaline unwinding, the duplex form of DNA decreased under the influence of CY-A, CY-E, GLU, MPG, NEM, NAP, NFA, araC, FU, AM, E, and BM. It is suggested that stimulation of unscheduled DNA synthesis combined with a transient decrease of semiconservative DNA synthesis will amplify the DNA repair capacity of thymocytes, whereas radiation damage may be intensified by araC, FU, AM,E, and DDC - at least partly, through inhibition of unscheduled DNA synthesis. With respect to the action of NAP, NFA, and BM, DNA repair may be concerned in a more indirect manner.

  8. Ceramide synthase 2 facilitates S1P-dependent egress of thymocytes into the circulation in mice.

    Science.gov (United States)

    Rieck, Michael; Kremser, Christiane; Jobin, Katarzyna; Mettke, Elisabeth; Kurts, Christian; Gräler, Markus; Willecke, Klaus; Kolanus, Waldemar

    2017-04-01

    Well-defined gradients of the lipid mediator sphingosine-1-phosphate (S1P) direct chemotactic egress of mature thymocytes from the thymus into the circulation. Although it is known that these gradients result from low S1P levels in the thymic parenchyma and high S1P concentrations at the exit sites and in the plasma, the biochemical mechanisms that regulate these differential S1P levels remain unclear. Several studies demonstrated that ceramide synthase 2 (Cers2) regulates the levels of the S1P precursor sphingosine. We, therefore, investigated whether Cers2 is involved in the regulation of S1P gradients and S1P-dependent egress into the circulation. By analyzing Cers2-deficient mice, we demonstrate that Cers2 limits the levels of S1P in thymus and blood to maintain functional S1P gradients that mediate thymocyte emigration into the circulation. This function is specific for Cers2, as we also show that Cers4 is not involved in the regulation of thymic egress. Our study identified Cers2 as an important regulator of S1P-dependent thymic egress, and thus contributes to the understanding of how S1P gradients are maintained in vivo. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Association of HLA-DP/DQ and STAT4 polymorphisms with HBV infection outcomes and a mini meta-analysis.

    Directory of Open Access Journals (Sweden)

    Yun Liao

    Full Text Available BACKGROUND: Though HLA-DP/DQ is regarded to associate with HBV susceptibility and HBV natural clearance, its role in hepatocellular carcinoma (HCC development is obscure. And the role of STAT4 in HBV susceptibility and clearance as well as HCC development is still contentious. Therefore, we conducted this study, aiming to clarify these obscure relationships. METHODS: We recruited 1312 Chinese Han subjects including healthy controls, HBV carriers and HCC patients in the experiment stage. The meta-analysis included 3467 HCC patients and 5821 HBV carriers to appraise the association with HCC development. RESULTS: Consistent with previous studies, HLA-DP/DQ associated with HBV susceptibility and HBV natural clearance (p<0.05. However, the experiment showed that HLA-DP rs3077, rs9277535 and rs7453920 did not associate with HCC development (dominant model, rs3077, OR = 0.86, 95%CI = 0.62-1.18; rs9277535, OR = 0.94, 95%CI = 0.68-1.30; rs7453920, OR = 0.75, 95%CI = 0.44-1.27. Meta-analysis again consolidated this conclusion (allele model, rs3077, OR = 0.94, 95%CI = 0.87-1.02; rs9277535, OR = 1.04, 95%CI = 0.97-1.11; rs7453920, OR = 0.89, 95%CI = 0.76-1.02. As for STAT4 rs7574865, we did not find any significant association with HBV susceptibility (OR = 0.91, 95%CI = 0.66-1.26 or HBV natural clearance (OR = 1.13, 95%CI = 0.86-1.49. Moreover, current data failed to acquire positive connection of rs7574865 with HCC development (experiment, OR = 0.86, 95%CI = 0.62-1.19; meta-analysis, OR = 0.87, 95%CI = 0.74-1.03, which may be due to the small sample size. CONCLUSIONS: HLA-DP/DQ polymorphisms (rs3077, rs9277535, rs7453920 did not associate with HCC development, but did correlate with HBV susceptibility and HBV natural clearance. STAT4 rs7574865 seemed not to correlate with HBV susceptibility or natural clearance. And it seemed rather ambiguous in its role on HCC development at

  10. Mtf-1 lymphoma-susceptibility locus affects retention of large thymocytes with high ROS levels in mice after γ-irradiation

    International Nuclear Information System (INIS)

    Maruyama, Masaki; Yamamoto, Takashi; Kohara, Yuki; Katsuragi, Yoshinori; Mishima, Yukio; Aoyagi, Yutaka; Kominami, Ryo

    2007-01-01

    Mouse strains exhibit different susceptibilities to γ-ray-induced thymic lymphomas. Our previous study identified Mtf-1 (metal responsive transcription factor-1) as a candidate susceptibility gene, which is involved in the radiation-induced signaling pathway that regulates the cellular reactive oxygen species (ROS). To reveal the mechanism for the increased susceptibility conferred by Mtf-1 locus, we examined early effects of γ-ray on ROS levels in vivo and its difference between Mtf-1 susceptible and resistant congenic mice. Here, we show the detection of clonally growing thymocytes at 4 weeks after irradiation, indicating the start of clonal expansion at a very early stage. We also show that large thymocytes with higher ROS levels and a proliferation capacity were more numerous in the Mtf-1 susceptible mice than the resistant mice when examined at 7 days after irradiation, although such tendency was not found in mice lacking one allele of Bcl11b tumor suppressor gene. This high retention of the large thymocytes, at a high risk for ROS-induced mutation, is a compensatory proliferation and regeneration response to depletion of the thymocytes after irradiation and the response is likely to augment the development of prelymphoma cells leading to thymic lymphomas

  11. Effects of tri-n-butyltin chloride on energy metabolism, macromolecular synthesis, precursor uptake and cyclic AMP production in isolated rat thymocytes

    NARCIS (Netherlands)

    Snoeij, N.J.; Punt, P.M.; Penninks, A.H.; Seinen, W.

    1986-01-01

    The inhibitor of oxidative phosphorylation tri-n-butyltin chloride (TBTC) causes membrane damage and disintegration of isolated rat thymocytes at concentrations higher than 1 μM. From a concentration of 0.1 μM, TBTC disturbs energy metabolism as indicated by an increase in methylglucose uptake,

  12. TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells.

    Directory of Open Access Journals (Sweden)

    Ana Rosa Pérez

    Full Text Available Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental Chagas disease. Migratory activity of thymocytes and mature T cells seem to be finely tuned by cytokines, chemokines and extracellular matrix (ECM components. Systemic TNF-α is enhanced during infection and appears to be crucial in the response against the parasite. However, it also seems to be involved in disease pathology, since it is implicated in the arrival of T cells to effector sites, including the myocardium. Herein, we analyzed the role of TNF-α in the migratory activity of thymocytes in Trypanosoma cruzi (T. cruzi acutely-infected mice. We found increased expression and deposition of TNF-α in the thymus of infected animals compared to controls, accompanied by increased co-localization of fibronectin, a cell migration-related ECM molecule, whose contents in the thymus of infected mice is also augmented. In-vivo studies showed an enhanced export of thymocytes in T. cruzi-infected mice, as ascertained by intrathymic injection of FITC alone or in combination with TNF-α. The increase of immature CD4(+CD8(+ T cells in secondary lymphoid organs was even more clear-cut when TNF-α was co-injected with FITC. Ex-vivo transmigration assays also revealed higher number of migrating cells when TNF-α was added onto fibronectin lattices, with higher input of all thymocyte subsets, including immature CD4(+CD8(+. Infected animals also exhibit enhanced levels of expression of both mRNA TNF-α receptors in the CD4(+CD8(+ subpopulation. Our findings suggest that in T. cruzi acute infection, when TNF-α is complexed with fibronectin, it favours the altered migration of thymocytes, promoting the release of mature and immature T cells to different compartments of the immune system. Conceptually, this work reinforces the notion that thymocyte migration is a multivectorial biological event

  13. Low Cycle Fatigue Behaviour of DP Steels: Micromechanical Modelling vs. Validation

    Directory of Open Access Journals (Sweden)

    Ghazal Moeini

    2017-07-01

    Full Text Available This study aims to simulate the stabilised stress-strain hysteresis loop of dual phase (DP steel using micromechanical modelling. For this purpose, the investigation was conducted both experimentally and numerically. In the experimental part, the microstructure characterisation, monotonic tensile tests and low cycle fatigue tests were performed. In the numerical part, the representative volume element (RVE was employed to study the effect of the DP steel microstructure of the low cycle fatigue behavior of DP steel. A dislocation-density based model was utilised to identify the tensile behavior of ferrite and martensite. Then, by establishing a correlation between the monotonic and cyclic behavior of ferrite and martensite phases, the cyclic deformation properties of single phases were estimated. Accordingly, Chaboche kinematic hardening parameters were identified from the predicted cyclic curve of individual phases in DP steel. Finally, the predicted hysteresis loop from low cycle fatigue modelling was in very good agreement with the experimental one. The stabilised hysteresis loop of DP steel can be successfully predicted using the developed approach.

  14. Microstructural investigations of the trimmed edge of DP980 steel sheets

    Science.gov (United States)

    Bhattacharya, S.; Green, D. E.; Sohmshetty, R.; Alpas, A. T.

    2017-10-01

    In order to reduce vehicle weight while maintaining crashworthiness, advanced high strength steels (AHSSs), such as DP980, are extensively used for manufacturing automotive body components. During trimming operations, the high tensile strength of DP980 sheets tends to cause damage of the trim edge of D2 die inserts, which result in deterioration of the edge quality. The objective of this work is to study the damage microstructures at the trimmed edge of DP980 steel sheets as a function of the number of trimming cycles. A mechanical press equipped with AISI D2 tool steel inserts was used to continuously trim 1.4 mm thick sheets of DP980 at a rate of 30 strokes/min. Cross-sectional SEM images of the trimmed edges revealed that the sheared edge quality of the DP980 sheets decreased, indicated by an increase in the burr width, with an increase in the number of trims from 40,000 to 70,000. Plastic strains were estimated using the displacements of the martensite plates within plastic flow fields of ferrite. Site-specific cross-sectional TEM samples, excised from the trimmed edge using the in-situ `lift-out' technique by focused ion-beam (FIB)-milling, revealed cracking at the ferrite/martensite interfaces after 70,000 cycles indicating an increase in the depth of deformation zone possibly due to trimming with a chipped and blunted die edge.

  15. Metabolism of neutral lipids in nuclei and chromatin of thymocytes from normal and γ-irradiated rats

    International Nuclear Information System (INIS)

    Kulagina, T.P.; Shuruta, S.A.; Kolomijtseva, I.K.

    1993-01-01

    The levels ans specific radioactivities of cholesterol and free fatty acids in nuclei and chromatin of thymocytes from normal and γ-irradiated (10 Gy) rats have been studied. The radioactivity of the total lipid fraction of γ-irradiated cells was decreased significantly in the absence of inhibition of [2- 14 C]acetete incorporation into the total proteil and lipid reactions and the [ 3 H]uracyl incorporation into the acid-insoluble RNA. The concentration of free fatty acids in the nuclei increased significantly after irradiation. The specific radioactivity of cholesterol in chromatin was higher than in the nuclei. The differences in specific radioactivities of free fatty acids were less pronounced. After irradiation the ratio of specific radioactivities of free fatty acids in chromatin to that in the nucleai showed a tendency to increase

  16. Proper acceleration, the geometric tachyon and the dynamics of a fundamental string near Dp branes

    International Nuclear Information System (INIS)

    Das, Ashok; Panda, Sudhakar; Roy, Shibaji

    2009-01-01

    We present a detailed analysis of our recent observation that the origin of the geometric tachyon, which arises when a Dp brane propagates in the vicinity of a stack of coincident NS5 branes, is due to the proper acceleration generated by the background dilaton field. We show that when a fundamental string (F-string), described by the Nambu-Goto action, is moving in the background of a stack of coincident Dp branes, the geometric tachyon mode can also appear since the overall conformal mode of the induced metric for the string can act as a source for proper acceleration. We also studied the detailed dynamics of the F-string as well as the instability by mapping the Nambu-Goto action of the F-string to the tachyon effective action of the non-BPS D-string. We qualitatively argue that the condensation of the geometric tachyon is responsible for the (F,Dp) bound state formation.

  17. Using the DP-190 glue for adhesive attachment of a large space mirror and its rim

    Science.gov (United States)

    Vlasenko, Oleg; Zverev, Alexey; Sachkov, Mikhail

    2014-07-01

    The glue DP-190 is widely used for adhesive attachment of astrositall (zerodur) lightweight large-size space astronomical mirrors (diameter of 1.7 m and more) with elements of their frames of invar. Peculiarities of physicalmechanical behavior of the glue DP-190 when exposed to the environment during the ground operation and in orbit cause instability of the reflective surface quality of mirrors. In this report we show that even a small (around 1%-5%) volumetric deformation of a cylindrical adhesive layer with a thickness of 0.8 mm between the mirror and the rim element causes significant mirrors deformation. We propose to use adhesive layer of special form that allows to reduce volumetric deformations of the glue DP-190 up to three times. Here we present results based on primary mirror tests of the WSO-UV project.

  18. NARDAC Civilian DP Training: A Need for Management Attention.

    Science.gov (United States)

    1982-12-01

    POSITION DESCRIPTIONS ------------------------ 28 D. TRAINING ------------------------------------- 31 IV. NARDAC ORGANIZATIONAL DIAGNOSIS ------------------ 32...the production schedule. 31 IV. NARDAC ORGANIZATIONAL DIAGNOSIS The upper level manager of a highly sophisticated, production oriented ADP facility...14 September 1982. 22. Preziosi, R.C., " Organizational Diagnosis Questicnnaire", The 1990 Annual Handbook for Group Facilitators, p. 112, University

  19. High phase noise tolerant pilot-tone-aided DP-QPSK optical communication systems

    DEFF Research Database (Denmark)

    Zhang, Xu; Pang, Xiaodan; Deng, Lei

    2012-01-01

    In this paper we experimentally demonstrate a novel, high phase-noise tolerant, optical dual polarization (DP) quadrature phase-shift keying (QPSK) communication system based on pilot-tone-aided phase noise cancellation (PNC) algorithm. Vertical cavity surface emitting lasers (VCSELs) with approx......In this paper we experimentally demonstrate a novel, high phase-noise tolerant, optical dual polarization (DP) quadrature phase-shift keying (QPSK) communication system based on pilot-tone-aided phase noise cancellation (PNC) algorithm. Vertical cavity surface emitting lasers (VCSELs...

  20. Influence of stress triaxiality and strain rate on the failure behavior of a dual-phase DP780 steel

    International Nuclear Information System (INIS)

    Anderson, D.; Winkler, S.; Bardelcik, A.; Worswick, M.J.

    2014-01-01

    Highlights: • DP780 steel sheet sensitive to strain rate and triaxiality. • Specimens failed due to ductile-shear mode. • Extent of transverse cracking due to martensitic islands increased with triaxiality. • Uniaxial stress decreased with strain rate then increased after 0.1 s −1 . • Predicted effective plastic strain, triaxiality at failure increased with strain rate. - Abstract: To better understand the in-service mechanical behavior of advanced high-strength steels, the influence of stress triaxiality and strain rate on the failure behavior of a dual-phase (DP) 780 steel sheet was investigated. Three flat, notched mini-tensile geometries with varying notch severities and initial stress triaxialities of 0.36, 0.45, and 0.74 were considered in the experiments. Miniature specimens were adopted to facilitate high strain rate testing in addition to quasi-static experiments. Tensile tests were conducted at strain rates of 0.001, 0.01, 0.1, 1, 10, and 100 s −1 for all three notched geometries and compared to mini-tensile uniaxial samples. Additional tests at a strain rate of 1500 s −1 were performed using a tensile split Hopkinson bar apparatus. The results showed that the stress–strain response of the DP780 steel exhibited mainly positive strain rate sensitivity for all geometries, with mild negative strain rate sensitivity up to 0.1 s −1 for the uniaxial specimens. The strain at failure was observed to decrease with strain rate at low strain rates of 0.001–0.1 s −1 ; however, it increased by 26% for an increase in strain rate from 0.1 to 1500 s −1 for the uniaxial condition. Initial triaxiality was found to have a significant negative impact on true failure strain with a decrease of 32% at the highest triaxiality compared to the uniaxial condition at a strain rate of 0.001 s −1 . High resolution scanning electron microscopy images of the failure surfaces revealed a dimpled surface while optical micrographs revealed shearing through the

  1. Application of the singularity subtraction method for (d,p) reactions on light nuclei

    International Nuclear Information System (INIS)

    Borbely, I.

    1974-01-01

    It is shown that the method of subtraction the nearest singularity can be used successfully for data processing of (d,p) reactions. The data on the nuclear structure, thus obtained, can then be used for a more efficient study of the reaction mechanism with the use of traditional methods

  2. Engineering Task Plan for Hepa Filter Differential Pressure (DP) Fan Interlock Upgrades

    International Nuclear Information System (INIS)

    SIMONS, S.R.

    2000-01-01

    This document provides a plan for installation of Differential Pressure (DP) fan interlocks on the primary ventilation systems in selected Tank Farm facilities. This plan contains the engineering tasks required for installation and is summarized by the Acceptance for Beneficial Use list. Individuals responsible for each task are identified and scheduled accordingly

  3. Ckmeans.1d.dp: Optimal k-means Clustering in One Dimension by Dynamic Programming.

    Science.gov (United States)

    Wang, Haizhou; Song, Mingzhou

    2011-12-01

    The heuristic k -means algorithm, widely used for cluster analysis, does not guarantee optimality. We developed a dynamic programming algorithm for optimal one-dimensional clustering. The algorithm is implemented as an R package called Ckmeans.1d.dp . We demonstrate its advantage in optimality and runtime over the standard iterative k -means algorithm.

  4. Year 2000 compliance concerns with the ISA Thermoluminescent Dosimetry Data Processing (TL-DP) software system

    Energy Technology Data Exchange (ETDEWEB)

    Saviz, K.

    1998-05-26

    The year 2000 is rapidly approaching, and there is a good chance that computer systems that utilize two digit year dates will experience problems in retrieval of date information. The ISA Thermoluminescent Dosimetry Data Processing (TL-DP) software and computer system has been reviewed for Year 2000 compliance issues.

  5. Year 2000 compliance concerns with the ISA Thermoluminescent Dosimetry Data Processing (TL-DP) software system

    International Nuclear Information System (INIS)

    Saviz, K.

    1998-01-01

    The year 2000 is rapidly approaching, and there is a good chance that computer systems that utilize two digit year dates will experience problems in retrieval of date information. The ISA Thermoluminescent Dosimetry Data Processing (TL-DP) software and computer system has been reviewed for Year 2000 compliance issues

  6. Synergistic effects of antimicrobial peptide DP7 combined with antibiotics against multidrug-resistant bacteria

    Directory of Open Access Journals (Sweden)

    Wu X

    2017-03-01

    Full Text Available Xiaozhe Wu,1 Zhan Li,1 Xiaolu Li,2,3 Yaomei Tian,1 Yingzi Fan,1 Chaoheng Yu,1 Bailing Zhou,1 Yi Liu,4 Rong Xiang,5 Li Yang1 1State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, 2International Center for Translational Chinese Medicine, Sichuan Academy of Chinese Medicine Sciences, Chengdu, 3Department of Plastic and Burn Surgery, Affiliated Hospital of Southwest Medical University, Luzhou, 4Department of Microbial Examination, Sichuan Center for Disease Control and Prevention, Chengdu, 5Nankai University School of Medicine, Tianjin, People’s Republic of China Abstract: Antibiotic-resistant bacteria present a great threat to public health. In this study, the synergistic effects of antimicrobial peptides (AMPs and antibiotics on several multidrug-resistant bacterial strains were studied, and their synergistic effects on azithromycin (AZT-resistance genes were analyzed to determine the relationships between antimicrobial resistance and these synergistic effects. A checkerboard method was used to evaluate the synergistic effects of AMPs (DP7 and CLS001 and several antibiotics (gentamicin, vancomycin [VAN], AZT, and amoxicillin on clinical bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli. The AZT-resistance genes (ermA, ermB, ermC, mefA, and msrA were identified in the resistant strains using quantitative polymerase chain reaction. For all the clinical isolates tested that were resistant to different antibiotics, DP7 had high antimicrobial activity (≤32 mg/L. When DP7 was combined with VAN or AZT, the effect was most frequently synergistic. When we studied the resistance genes of the AZT-resistant isolates, the synergistic effect of DP7–AZT occurred most frequently in highly resistant strains or strains carrying more than two AZT-resistance genes. A transmission electron microscopic analysis of the S. aureus

  7. Effects of ionizing radiation on expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice

    International Nuclear Information System (INIS)

    Ni Guanying; Wu Ning; Guo Haizhuo; Jin Shunzi

    2011-01-01

    Objective: To investigate the effects of ionizing radiation on the expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice. Methods: Flow cytometry (FCM) was used to analyze the expression of P21 protein in Jurkat cells at 12 and 24 h after irradiation to 0, 0.5, 1.0, 2.0, 4.0, and 6.0 Gy. Real-time PCR was used to detect the expression of p21 gene in thymocytes and splenocytes of mice at 4 and 24 h after irradiation to 0, 0.5, 1.0, 2.0, 4.0, and 6.0 Gy. Multi-staining was used to analyze the micronucleus rates of Rct in bone marrow. Results: The expressions of P21 protein were increased in a dose-dependent manner during 0.5-4.0 Gy (t=-24.23 - -3.96, P<0.05), but decreased at 6.0 Gy at 12 and 24 h post-irradiation (t=-11.19, -14.50, P<0.05). The expressions of p21 gene in both thymocytes and splenocytes of mice were increased in dose-dependent manner in the range of 0-6.0 Gy (including 6.0 Gy) (t=-29.96-8.80, P<0.05), and reached to the peak at 6.0 Gy at 4 and 24 h post-irradiation (t=-11.84 - -3.42, P<0.05), except thymocytes at 4 h and 1.0 Gy post-irradiation (t=-3.42, P>0.05). Conclusions: The expressions of P21 protein and p21 gene could be increased by X-ray irradiation, which shows good dose-dependent manners in certain range of dose. (authors)

  8. The influence of blood plasma of irradiated animals on activity of Ca2+ - ATPase and Mg2+ - ATPase in plasma membrane of thymocytes

    International Nuclear Information System (INIS)

    Dreval', V.I.

    1994-01-01

    Rats were irradiated at doses 1.5, 4.0, 7.0 and 10 Gy. After 1, 8, 15, 22 and 30 days the effect of blood plasma on activity of Ca 2+ -ATPase and Mg 2+ -ATPase in plasma membrane of thymocytes was investigated. It was found that the raise of irradiation dose leads to increasing of blood plasma effect on membrane-bound enzymes

  9. Characterizing the binding motifs of 11 common human HLA‐DP and HLA‐DQ molecules using NNAlign

    DEFF Research Database (Denmark)

    Andreatta, Massimo; Nielsen, Morten

    2012-01-01

    ‐based method NNAlign, we characterized the binding specificities of five HLA‐DP and six HLA‐DQ among the most frequent in the human population. The identified binding motifs showed an overall concurrence with earlier studies but revealed subtle differences. The DP molecules revealed a large overlap...

  10. The molecular origin of the thiamin diphosphate-induced spectral bands of ThDP-dependent enzymes

    NARCIS (Netherlands)

    Kovina, M.V.; Kok, A.; Sevostyanova, I.A.; Khailova, L.S.; Belkina, N.V.; Kochetov, G.A.

    2004-01-01

    New and previously published data on a variety of ThDP-dependent enzymes such as baker's yeast transketolase, yeast pyruvate decarboxylase and pyruvate dehydrogenase from pigeon breast muscle, bovine heart, bovine kidney, Neisseria meningitidis and E. coli show their spectral sensitivity to ThDP

  11. [Quantitative analysis of Cu in water by collinear DP-LIBS].

    Science.gov (United States)

    Zheng, Mei-Lan; Yao, Ming-Yin; Chen, Tian-Bing; Lin, Yong-Zeng; Li, Wen-Bing; Liu, Mu-Hua

    2014-07-01

    The purpose of this research is to study the influence of double pulse laser induced breakdown spectroscopy (DP-LIBS) on the sensitivity of Cu in water. The water solution of Cu was tested by collinear DP-LIBS in this article. The results show that spectral intensity of Cu can be enhanced obviously by DP-LIBS, compared with single pulse laser induced breakdown spectroscopy (SP-LIBS). Besides, the experimental results were significantly impacted by delay time between laser pulse and spectrometer acquisition, delay time of double laser pulse and energy of laser pulse and so on. The paper determined the best conditions for DP-LIBS detecting Cu in water. The optimal acquisition delay time was 1 380 ns. The best laser pulse delay time was 25 ns. The most appropriate energy of double laser pulse was 100 mJ. Characteristic analysis of spectra of Cu at 324.7 and 327.4 nm was done for quantitative analysis. The detection limit was 3.5 microg x mL(-1) at 324.7 nm, and the detection limit was 4.84 microg x mL(-1) at 327.4 nm. The relative standard deviation of the two characteristic spectral lines was within 10%. The calibration curve of characteristic spectral line, established by 327.4 nm, was verified with 500 microg x mL(-1) sample. Concentration of the sample was 446 microg x mL(-1) calculated by the calibration curve. This research shows that the detection sensitivity of Cu in water can be improved by DP-LIBS. At the same time, it had high stability.

  12. Constitutive expression of tert in thymocytes leads to increased incidence and dissemination of T-cell lymphoma in Lck-Tert mice.

    Science.gov (United States)

    Canela, Andrés; Martín-Caballero, Juan; Flores, Juana M; Blasco, María A

    2004-05-01

    Here we describe a new mouse model with constitutive expression of the catalytic subunit of telomerase (Tert) targeted to thymocytes and peripheral T cells (Lck-Tert mice). Two independent Lck-Tert mouse lines showed higher incidences of spontaneous T-cell lymphoma than the corresponding age-matched wild-type controls, indicating that constitutive expression of Tert promotes lymphoma. Interestingly, T-cell lymphomas in Lck-Tert mice were more disseminated than those in wild-type controls and affected both lymphoid and nonlymphoid tissues, while nonlymphoid tissues were never affected with lymphoma in age-matched wild-type controls. Importantly, these roles of Tert constitutive expression in promoting tumor progression and dissemination were independent of the role of telomerase in telomere length maintenance, since telomere length distributions on a single-cell basis were identical in Lck-Tert and wild-type thymocytes. Finally, Tert constitutive expression did not interfere with telomere capping in Lck-Tert primary thymocytes, although it resulted in greater chromosomal instability upon gamma irradiation in Lck-Tert primary lymphocytes than in controls, suggesting that Tert overexpression may interfere with the cellular response to DNA damage.

  13. Levels, profile and distribution of Dechloran Plus (DP) and Polybrominated Diphenyl Ethers (PBDEs) in the environment of Pakistan.

    Science.gov (United States)

    Syed, Jabir Hussain; Malik, Riffat Naseem; Li, Jun; Wang, Yan; Xu, Yue; Zhang, Gan; Jones, Kevin C

    2013-11-01

    No scientific data is available on emerging contaminants including Polybrominated Diphenyl Ethers (PBDEs) and Dechloran Plus (DP) levels in the environment in Pakistan. Levels of PBDEs and DP were determined in the soil, sediment and atmospheric samples along the stretch of River Ravi in Punjab Province. Average concentrations of ΣPBDEs in atmosphere, soils and sediments were 36 pg m(-3), 40 ng g(-1) and 640 ng g(-1). BDE-209 was the most abundant PBDE congener, showing that deca-BDE accounts for most of the total PBDE emitted in the environment of Pakistan. Total DP levels were calculated as 88 pg m(-3), 0.8 ng g(-1) and 1.9 ng g(-1) in air, soil and sediment samples, respectively. The lower average fractions of anti-DP showed significant differences to those of the technical mixtures, indicating the lack of DP production source in Pakistan. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. Cellular adverse actions of dibromoacetonitrile, a by-product in water bacterial control, at sublethal levels in rat thymocytes.

    Science.gov (United States)

    Kishida, Takumi; Akiyoshi, Kenji; Erdenedalai, Erdenebat; Enhetomuru, Anu; Imai, Shoji; Oyama, Yasuo

    2018-09-01

    The aim of this study was to investigate the effects of dibromoacetonitrile (DBAN), a by-product in water bacterial control, at sublethal concentrations on rat thymocytes, by using a cytometric technique with appropriate fluorescent dyes. By using this method, the possibility that DBAN induces cellular actions related to oxidative stress was assessed. DBAN reduced the content of cellular nonprotein thiols under Zn 2+ -free conditions. It elevated the intracellular level of Zn 2+ , being independent from external Zn 2+ . DBAN increased cell vulnerability to the cytotoxic action of hydrogen peroxide. These actions of DBAN were likely related to oxidative stress. DBAN is formed by the reaction of bromides and chlorinated oxidants during water disinfection. Hydrolysis of 2,2-dibromo-3-nitrilopropionamide, an antimicrobial used in hydraulic fracturing fluids for production of shale gas and oil, produces DBAN. Therefore, the concern regarding the levels of DBAN in industrial water systems is necessary to avoid the environmental risk to humans and wild mammals. Copyright © 2018 Elsevier Ltd. All rights reserved.

  15. Effect of some radioprotective and radiosensitizing substances on the semiconservative and unscheduled DNA biosynthesis of rat thymocytes

    International Nuclear Information System (INIS)

    Winkle, J.

    1981-01-01

    The effect on the semiconservative and unscheduled insertion of 3 H-methyl-thymidine (TdR- 3 H) into the DNA was tested on rat thymocytes in vitro. The semiconservative incorporation of TdR- 3 H was inhibited by AET, cysteine glutathione, N-ethylmaleimide, cytosine arabinoside, ethidiumbromide, bleomycin and diethyldithiocarbamate. Metronidazole and caffeine had no effect. Aminothiols and bleomycin stimulated, cytosine arabinoside, N-ethylmaleimide, ethidiumbromide and diethyldithiocarbamate decreased the unscheduled TdR- 3 H incorporation. There was no substantial effect of an exposure to UV-rays. The results lead to the following conclusions: The aminothiol-effect on the excision repair suggest that inhibition of the semiconservative DNA-synthesis will amplify regenerative capacity of the cells. The effect of most substances investigated accord with the present views on their mechanisms of action. The present investigations do not allow an explanation of the influence of diethyldithiocarbamate unspecific effects (such as complexing activity) and more specialized reactions (such as inhibition of superoxide dismutase) must be kept in mind. (orig./MG)

  16. Effects of ultraviolet radiation on the production of epidermal derived thymocyte-activating factor/interleukin-1

    International Nuclear Information System (INIS)

    Gahring, L.C.

    1986-01-01

    The effect of both a single exposure and multiple exposures to ultraviolet radiation (UVR) on the production and/or release of epidermal cell derived thymocyte activating factor/interleukin-1 (ETAF/IL-1) were investigated. A single exposure to UVR enhances the release of ETAF/IL-1 both in vitro and in vivo. This conclusion was based on the observation that: (1) in vitro UV-irradiation of the keratinocyte cell line elevated the release of ETAF/IL-1 on a per cell basis; (2) exposure of animals to UVR stimulated a number of in vivo biologic responses which are known to be mediated by ETAF/IL-1; and (3) ETAF/IL-1 could be detected in the serum of UVR exposed but not normal animals. Exposure of mice to UV-irradiation on a daily basis induced a desensitized state in which animals were found to be refractory to further stimulation with this inflammatory agent. A similar desensitization or tolerance was also shown to be induced by multiple intraperitoneal injections of bacterial lipopolysaccharide (LPS). Desensitization, induced by either LPS or UVR, was found to be regulated at the site of interaction between the cells capable of ETAF/IL-1 production and the exogenous inflammatory stimulus. The results indicate that the regulatory mechanisms for ETAF/IL-1 production which are employed by the keratinocyte are distinct from those regulating ETAF/Il-1 production by the macrophage

  17. scid Thymocytes with TCRbeta gene rearrangements are targets for the oncogenic effect of SCL and LMO1 transgenes.

    Science.gov (United States)

    Chervinsky, D S; Lam, D H; Melman, M P; Gross, K W; Aplan, P D

    2001-09-01

    SCL and LMO1 were both discovered by virtue of their activation by chromosomaltranslocation in patients with T-cell acute lymphoblastic leukemia (T-ALL). Overexpression of SCL and LMO1 in the thymus of transgenic mice leads to T-ALL at a young age. scid (severe combined immunodeficient) mice are unable to efficiently recombine antigen receptor genes and consequently display a developmental block at the CD4-CD8- to CD4+CD8+ transition. To test the hypothesis that this developmental block would protect SCL/LMO1 transgenic mice from developing T-ALL, we crossed the SCL and LMO1 transgenes onto a scid background. The age of onset for T-ALL in the SCL/LMO1/scid mice was significantly delayed (P < 0.001) compared with SCL/LMO1/wild-type mice. Intriguingly, all of the SCL/LMO1/scid malignancies displayed clonal, in-frame TCRbeta gene rearrangements. Taken together, these findings suggest that the "leaky" scid thymocyte that undergoes a productive TCRbeta gene rearrangement is susceptible to the oncogenic action of SCL and LMO1 and additionally suggests that TCRbeta gene rearrangements may be required for the oncogenic action of SCL and LMO1.

  18. A mechanism for overcoming P-glycoprotein-mediated drug resistance: novel combination therapy that releases stored doxorubicin from lysosomes via lysosomal permeabilization using Dp44mT or DpC.

    Science.gov (United States)

    Seebacher, Nicole A; Richardson, Des R; Jansson, Patric J

    2016-12-01

    The intracellular distribution of a drug can cause significant variability in both activity and selectivity. Herein, we investigate the mechanism by which the anti-cancer agents, di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) and the clinically trialed, di-2-pyridylketone 4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC), re-instate the efficacy of doxorubicin (DOX), in drug-resistant P-glycoprotein (Pgp)-expressing cells. Both Dp44mT and DpC potently target and kill Pgp-expressing tumors, while DOX effectively kills non-Pgp-expressing cancers. Thus, the combination of these agents should be considered as an effective rationalized therapy for potently treating advanced and resistant tumors that are often heterogeneous in terms of Pgp-expression. These studies demonstrate that both Dp44mT and DpC are transported into lysosomes via Pgp transport activity, where they induce lysosomal-membrane permeabilization to release DOX trapped within lysosomes. This novel strategy of loading lysosomes with DOX, followed by permeabilization with Dp44mT or DpC, results in the relocalization of stored DOX from its lysosomal 'safe house' to its nuclear targets, markedly enhancing cellular toxicity against resistant tumor cells. Notably, the combination of Dp44mT or DpC with DOX showed a very high level of synergism in multiple Pgp-expressing cell types, for example, cervical, breast and colorectal cancer cells. These studies revealed that the level of drug synergy was proportional to Pgp activity. Interestingly, synergism was ablated by inhibiting Pgp using the pharmacological inhibitor, Elacridar, or by inhibiting Pgp-expression using Pgp-silencing, demonstrating the importance of Pgp in the synergistic interaction. Furthermore, lysosomal-membrane stabilization inhibited the relocalization of DOX from lysosomes to the nucleus upon combination with Dp44mT or DpC, preventing synergism. This latter observation demonstrated the importance of lysosomal

  19. Microstructure and mechanical properties of resistance-spot-welded joints for A5052 aluminum alloy and DP 600 steel

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Jianbin [College of Automotive Collaborative Innovation Center, Chongqing University, No. 174, Shazheng Street, Shapingba District, Chongqing 400044 (China); Yuan, Xinjian, E-mail: xinjianyuan@yahoo.com [College of Materials Science and Engineering, Chongqing University, No. 174, Shazheng Street, Shapingba District, Chongqing 400044 (China); Hu, Zhan; Sun, Changzheng; Zhang, Yanxin; Zhang, Yuxuan [College of Materials Science and Engineering, Chongqing University, No. 174, Shazheng Street, Shapingba District, Chongqing 400044 (China)

    2016-10-15

    The microstructure and mechanical properties of resistance-spot-welded A5052 aluminum alloy and DP 600 dual-phase steel joint were studied. The fusion zone (FZ) and heat-affected zone (HAZ) of DP 600 exhibited lath martensite and ferrite-martensite structures, respectively. The microstructure of FZ and HAZ in the A5052 side was composed of cellular crystals and the boundary region of FZ exhibited a columnar crystal morphology. A Fe{sub 2}Al{sub 5} intermetallic compound (IMC) layer with 3.3 μm thickness was found adjacent to the DP 600 side, whereas a needle-shaped Fe{sub 4}Al{sub 13} IMC layer with length of 0.67 μm to 15.8 μm was found adjacent to the aluminum alloy side. The maximum tensile shear load of the A5052/DP 600 joint was 5.5 KN, with a corresponding molten nugget diameter of 6.3 mm. The fracture morphology of the optimized A5052/DP 600 joint was mainly an elongated dimple fracture accompanied by cleavage fracture. - Highlights: •A5052 and DP 600 with large gaps in properties were investigated by RSW. •The microstructures of RSW joints in DP 600/A5052 were examined detailedly. •The micro/macro-characteristics and strength relations of joints were analyzed.

  20. Characterization of gas metal arc welded hot rolled DP600 steel

    Energy Technology Data Exchange (ETDEWEB)

    Mukherjee, K.; Ramazani, A.; Yang, L.; Prahl, U.; Bleck, W. [RWTH Aachen University, Institute for Ferrous Metallurgy (IEHK) (Germany); Reisgen, U.; Schleser, M.; Abdurakhmanov, A. [RWTH Aachen University, Welding and Joining Institute (ISF) (Germany)

    2011-12-15

    Dual-phase (DP) steels are suitable candidates for automotive applications due to their high strength and ductility. These advanced mechanical properties result from the special microstructure of the DP steel with 5{proportional_to}20% martensite phase in a soft ferrite matrix. However, during welding, which is an important process in automotive industry, this special microstructure is destroyed. In this research the characterization of Gas Metal Arc (GMA) welded joining zones was performed by optical microscopy and hardness mapping. Tensile tests were also performed keeping the welded portion in the gauge length. Scanning Electron Microscopy (SEM) was used for the fracture investigation. From the characterization and tensile tests, the soften zones were found, which are caused by the tempered martensite and larger ferrite grain size than that in base metal. Furthermore, GMA welding make a large Heat Affected Zone (HAZ). (Copyright copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  1. Architecture, Voltage, and Components for a Turboelectric Distributed Propulsion Electric Grid (AVC-TeDP)

    Science.gov (United States)

    Gemin, Paul; Kupiszewski, Tom; Radun, Arthur; Pan, Yan; Lai, Rixin; Zhang, Di; Wang, Ruxi; Wu, Xinhui; Jiang, Yan; Galioto, Steve; hide

    2015-01-01

    The purpose of this effort was to advance the selection, characterization, and modeling of a propulsion electric grid for a Turboelectric Distributed Propulsion (TeDP) system for transport aircraft. The TeDP aircraft would constitute a miniature electric grid with 50 MW or more of total power, two or more generators, redundant transmission lines, and multiple electric motors driving propulsion fans. The study proposed power system architectures, investigated electromechanical and solid state circuit breakers, estimated the impact of the system voltage on system mass, and recommended DC bus voltage range. The study assumed an all cryogenic power system. Detailed assumptions within the study include hybrid circuit breakers, a two cryogen system, and supercritical cyrogens. A dynamic model was developed to investigate control and parameter selection.

  2. Investigation of Droplet Deposition for Suspensions Usable for Thermoplastic 3D Printing (T3DP)

    Science.gov (United States)

    Scheithauer, Uwe; Johne, Robert; Weingarten, Steven; Schwarzer, Eric; Richter, Hans-Jürgen; Moritz, Tassilo; Michaelis, Alexander

    2018-01-01

    Thermoplastic 3D printing (T3DP) is an additive manufacturing (AM) technology, which can be used for the production of dense single- and especially multi-material components. This becomes possible because of the combination of the precise deposition of small droplets of molten thermoplastic suspensions containing ceramic or metal particles, and a curing mechanism caused on cool down increasing the viscosity. In this paper, the droplet formation behavior of zirconia suspensions for T3DP (82 and 84 wt.%) was investigated. The droplet fusion factor (dff) is introduced to calculate the necessary distance between two droplets to form filament-like structures by fusion of adjacent droplets. Filament-like structures with a smooth surface and a nearly homogeneous cross section were manufactured for both suspensions with a dff of 44% or higher.

  3. Polarization Observables for the Collinear dp → 3 Heπ0 Reaction

    International Nuclear Information System (INIS)

    Ladygin, V.P.; Ladygina, N.B.

    1994-01-01

    Effects due to polarizations of both colliding particles have been analyzed in terms of two independent amplitudes which in the general case define the spin structure of the amplitude of the dp → 3 Heπ 0 reaction in collinear geometry. The energy dependence of spin-correlation C L , L , O , O due to longitudinal polarization of colliding particles is predicted using the moduli of amplitudes extracted from experimental data. The limit of possible deviations is obtained for spin-correlation C N , N , O , O due to transverse polarization of both particles. The value of these polarization observables at threshold are predicted. The behaviour of these polarization observables for the dp → 3 Heη 0 reaction, having the same spin structure, is discussed. 22 refs., 5 figs., 2 tabs

  4. A DOE/DP multisite approach to safeguards integration with facility operations

    International Nuclear Information System (INIS)

    Ostenak, C.A.

    1987-01-01

    The Accountability Technology Exchange (ATEX) Working Group was established in October 1986 by the plutonium processing technology exchange steering committee under the US Department of Energy/Department of Defense program's materials management executive committee (DOE/DP MMEC). The ATEX Working Group provides a multisite, multidisciplinary forum for discussing, evaluating, and recommending both existing and emerging nuclear materials accountability measurement technologies for implementation into DOE/DP plutonium processing facilities. This forum assists technologists in understanding state-of-the-art plutonium processing and accountability measurement practices throughout the complex, as well as current and projected technical needs. As a result, the ATEX Working Group will contribute to the improvement of plutonium processing technologies by helping to assure that the best possible nuclear materials accountability measurement techniques are made available to all sites to process plutonium safely, securely, and cost-effectively

  5. Comparison of the carboxy-terminal DP-repeat region in the co-chaperones Hop and Hip.

    Science.gov (United States)

    Nelson, Gregory M; Huffman, Holly; Smith, David F

    2003-01-01

    Functional steroid receptor complexes are assembled and maintained by an ordered pathway of interactions involving multiple components of the cellular chaperone machinery. Two of these components, Hop and Hip, serve as co-chaperones to the major heat shock proteins (Hsps), Hsp70 and Hsp90, and participate in intermediate stages of receptor assembly. In an effort to better understand the functions of Hop and Hip in the assembly process, we focused on a region of similarity located near the C-terminus of each co-chaperone. Contained within this region is a repeated sequence motif we have termed the DP repeat. Earlier mutagenesis studies implicated the DP repeat of either Hop or Hip in Hsp70 binding and in normal assembly of the co-chaperones with progesterone receptor (PR) complexes. We report here that the DP repeat lies within a protease-resistant domain that extends to or is near the C-terminus of both co-chaperones. Point mutations in the DP repeats render the C-terminal regions hypersensitive to proteolysis. In addition, a Hop DP mutant displays altered proteolytic digestion patterns, which suggest that the DP-repeat region influences the folding of other Hop domains. Although the respective DP regions of Hop and Hip share sequence and structural similarities, they are not functionally interchangeable. Moreover, a double-point mutation within the second DP-repeat unit of Hop that converts this to the sequence found in Hip disrupts Hop function; however, the corresponding mutation in Hip does not alter its function. We conclude that the DP repeats are important structural elements within a C-terminal domain, which is important for Hop and Hip function.

  6. Energetic materials research and development activities at Sandia National Laboratories supported under DP-10 programs

    Energy Technology Data Exchange (ETDEWEB)

    Ratzel, A.C. III

    1998-09-01

    This report provides summary descriptions of Energetic Materials (EM) Research and Development activities performed at Sandia National Laboratories and funded through the Department of Energy DP-10 Program Office in FY97 and FY98. The work falls under three major focus areas: EM Chemistry, EM Characterization, and EM Phenomenological Model Development. The research supports the Sandia component mission and also Sandia's overall role as safety steward for the DOE Nuclear Weapons Complex.

  7. Multi-master profibus dp modelling and worst case analysis-based evaluation

    OpenAIRE

    Salvatore Monforte; Eduardo Tovar; Francisco Vasques; Salvatore Cavalieri

    2002-01-01

    This paper provides an analysis of the real-time behaviour of the multi-master Profibus DP network. The analysis is based on the evaluation of the worst-case message response time and the results obtained are compared with those present in literature, pointing out its capability to perform a more accurate evaluation of the performance of the Profibus network. Copyright © 2002 IFAC.

  8. Investigation of the network delay on Profibus-DP based network

    OpenAIRE

    Yılmaz, C.; Gürdal, O.; Sayan, H.H.

    2008-01-01

    The mathematical model of the network-induced delay control systems (NDCS) is given. Also the role of the NDCS’s components such as controller, sensor and network environment on the network-induced delay are included in the mathematical model of the system. The network delay is investigated on Profibus-DP based network application and experimental results obtained are presented graphically. The experimental results obtained show that the network induced delay is randomly changed according to ...

  9. Designing real-time systems based on mono-master Profibus-DP networks

    OpenAIRE

    Monforte, Salvatore; Alves, Mário; Vasques, Francisco; Tovar, Eduardo

    2000-01-01

    Profibus networks are widely used as the communication infrastructure for supporting distributed computer-controlled applications. Most of the times, these applications impose strict real-time requirements. Profibus-DP has gradually become the preferred Profibus application profile. It is usually implemented as a mono-master Profibus network, and is optimised for speed and efficiency. The aim of this paper is to analyse the real-time behaviour of this class of Profibus networks...

  10. EnzDP: improved enzyme annotation for metabolic network reconstruction based on domain composition profiles.

    Science.gov (United States)

    Nguyen, Nam-Ninh; Srihari, Sriganesh; Leong, Hon Wai; Chong, Ket-Fah

    2015-10-01

    Determining the entire complement of enzymes and their enzymatic functions is a fundamental step for reconstructing the metabolic network of cells. High quality enzyme annotation helps in enhancing metabolic networks reconstructed from the genome, especially by reducing gaps and increasing the enzyme coverage. Currently, structure-based and network-based approaches can only cover a limited number of enzyme families, and the accuracy of homology-based approaches can be further improved. Bottom-up homology-based approach improves the coverage by rebuilding Hidden Markov Model (HMM) profiles for all known enzymes. However, its clustering procedure relies firmly on BLAST similarity score, ignoring protein domains/patterns, and is sensitive to changes in cut-off thresholds. Here, we use functional domain architecture to score the association between domain families and enzyme families (Domain-Enzyme Association Scoring, DEAS). The DEAS score is used to calculate the similarity between proteins, which is then used in clustering procedure, instead of using sequence similarity score. We improve the enzyme annotation protocol using a stringent classification procedure, and by choosing optimal threshold settings and checking for active sites. Our analysis shows that our stringent protocol EnzDP can cover up to 90% of enzyme families available in Swiss-Prot. It achieves a high accuracy of 94.5% based on five-fold cross-validation. EnzDP outperforms existing methods across several testing scenarios. Thus, EnzDP serves as a reliable automated tool for enzyme annotation and metabolic network reconstruction. Available at: www.comp.nus.edu.sg/~nguyennn/EnzDP .

  11. Performance monitoring for coherent DP-QPSK systems based on stokes vectors analysis

    Science.gov (United States)

    Louchet, Hadrien; Koltchanov, Igor; Richter, André

    2010-12-01

    We show how to estimate accurately the Jones matrix of the transmission line by analyzing the Stokes vectors of DP-QPSK signals. This method can be used to perform in-situ PMD measurement in dual-polarization QPSK systems, and in addition to the constant modulus algorithm (CMA) to mitigate polarization-induced impairments. The applicability of this method to other modulation formats is discussed.

  12. Explorando o DP: a presença da categoria NumP

    Directory of Open Access Journals (Sweden)

    Marina R.A. Augusto

    2012-11-01

    Full Text Available This paper focuses on NumP, an independent projection for number,which was proposed in pre-minimalist research (Picallo, 1991; Ritter,1991. Arguments for the relevance of maintaining it as anindependent projection in a minimalist framework, contrary toChomsky (1999, who assumes number is one of the j-featuresassociated to N, are provided. The consequences such presenceimposes for DP internal agreement phenomenon are discussed andsome alternatives to account for it are offered.

  13. Quantifying the effects of tempering on individual phase properties of DP980 steel with nanoindentation

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, G.; Zhang, F.; Ruimi, A.; Field, D. P.; Sun, X.

    2016-06-01

    We conduct a series of thermal and mechanical testing on a commercial dual phase (DP) 980 steel in order to quantify the effects of tempering on its individual phase properties. Tempering treatment is conducted at 250 °C and 400 °C for 60 minutes each. Ferrite and martensite grains are distinguished using electron backscatter diffraction (EBSD) and scanning probe microscopy (SPM), and the martensite volume fractions (MVF) are determined based on the image quality (IQ) map. Multi-scale indentation tests combined with a newly developed inverse method are used to obtain the individual phase flow properties in each tempered DP980 sample. The results show that, i) tempering significantly reduces martensite yield strength, while it only slightly reduces the ferrite yield strength; ii) tempering temperature has a more significant influence on the work hardening exponent of ferrite than that of martensite; iii) the elastic modulus of martensite is consistently higher than that of ferrite. As a validation, a simple rule of mixtures is used to verify the above-predicted individual phase flow stresses with the experimentally obtained overall true stress vs. true strain curves. The methodology and the corresponding results shown in this study can help guide the selection of tempering parameters in optimizing the mechanical properties of DP steels for their intended applications.

  14. Density-functional study on the equilibria in the ThDP activation.

    Science.gov (United States)

    Delgado, Eduardo J; Alderete, Joel B; Jaña, Gonzalo A

    2011-11-01

    The equilibria among the various ionization and tautomeric states involved in the activation of ThDP is addressed using high level density functional theory calculations, X3LYP/6-311++G(d,p)//X3LYP(PB)/6-31++G(d,p). This study provides the first theoretically derived thermodynamic data for the internal equilibria in the activation of ThDP. The role of the medium polarity on the geometry and thermodynamics of the diverse equilibria of ThDP is addressed. The media chosen are cyclohexane and water, as paradigms of apolar and polar media. The results suggest that all ionization and tautomeric states are accessible during the catalytic cycle, even in the absence of substrate, being APH(+) the form required to interconvert the AP and IP tautomers; and the generation of the ylide proceeds via the formation of the IP form. Additionally, the calculated ΔG° values allow to calculate all the equilibrium constants, including the pK(C2) for the thiazolium C2 atom whose ionization is believed to initiate the catalytic cycle.

  15. Induction by anti-thymocyte globulins in kidney transplantation: a review of the literature and current usage.

    Science.gov (United States)

    Malvezzi, Paolo; Jouve, Thomas; Rostaing, Lionel

    2015-10-01

    Preventing acute rejection (AR) after kidney transplantation is of utmost importance because an AR can have a negative impact on long-term allograft survival. Directory of Open Access Journals (DOAJ), Google Scholar, PubMed, EBSCO, and Web of Science have been searched. At the moment this can be done by using rabbit anti-thymocyte globulins (rATGs) as an induction therapy. However, because rATGs are associated with some deleterious side-effects, such as the opportunistic infections cytomegalovirus (CMV) and de novo post-transplant cancer, it is very important they are used optimally, i.e., at minimal doses that avoid many side-effects but still retain optimal treatment efficacy. Recent data show that the risk of CMV infection can be minimized using tacrolimus plus everolimus, and not tacrolimus plus mycophenolic acid, as the maintenance immunosuppression. The use of rATG is particularly valuable in; (a) sensitized patients; (b) in recipients from an expanded-criteria donor, thus enabling the introduction of calcineurin inhibitors at reduced doses; and (c) for patients where steroid avoidance is contemplated. However, we also need to consider that rATG may increase the risk of de novo cancer, even though recent data indicate this is unlikely and that any risk can be reduced by using mammalian target of rapamycin (mTOR) inhibitors instead of mycophenolic acid combined with low-dose calcineurin inhibitors. Even though rATGs do not improve long-term kidney-allograft survival, they may help reduce calcineurin-inhibitor dosage during the early post-transplant period and minimize the risk of AR.

  16. Comparison of daclizumab, an interleukin 2 receptor antibody, to anti-thymocyte globulin-Fresenius induction therapy in kidney transplantation.

    Science.gov (United States)

    Abou-Jaoude, Maroun M; Ghantous, Imad; Almawi, Wassim Y

    2003-07-01

    The efficacy and safety of daclizumab and anti-thymocyte globulin-Fresenius (ATG-F) as induction therapy in kidney transplantation (KT) were investigated in 45 KT performed in our center between March and May 2002. Group II (n=10) received daclizumab as induction therapy, and Group I (n=35) were induced with a single intraoperative bolus therapy of ATG-F. All patients were at low-risk, and the recipient and donor demographics, as well the immunosuppression regimen employed were comparable in both groups. Drug safety, assessed by the occurrence of side effects, was almost comparable in the two groups, except for more thrombocytopenia in Group II (P<0.0004). Acute rejection (AR) occurred in 10% in Group I and 11.4% in Group II (P=NS). There were more infections in Group II (42.8%) than in Group I (10%) (P<0.009). Bacterial and viral infections were more common in Group II (69 and 23%) than in Group I (10 and 0%) (P<0.05). The hospital stay was similar in both groups. Mean serum creatinine levels upon discharge, at 1, 3 and 6 months were: 1.23+/-0.11, 1.21+/-0.06, 1.25+/-0.11 and 1.35+/-0.08 in Group I and 2.18+/-0.43, 1.49+/-0.16, 1.49+/-0.16 and 1.35+/-0.08 in Group II, respectively. While better serum creatinine levels were observed in Group I upon discharge (P<0.048), this was due to the presence of more sensitized patients in Group II. The 6 months actuarial patient and graft survival were identical in both groups (100 and 100%, respectively). Although both daclizumab and ATG-F were effective and safe as induction therapy in KT, less bacterial and viral infections and lower early serum creatinine levels were noted in daclizumab-treated patients.

  17. Adaptation of D-P flap to the oro-facial fistula induced by radio-osteomyelitis of mandible

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, E.; Genba, R.; Hayatsu, Y.; Sunakawa, H.; Kohama, G. (Sapporo Medical Coll. (Japan))

    1980-06-01

    Intraoral partial resection of the mandible was performed on 3 patients with radiation-induced ostomyelitis and ostonecrosis of the mandible, and inflammation in the mandible disappeared. Residual oro-facial fistula was closed with D-P flap, and a good result was obtained. Treatments of radiation osteopathy, the time of the treatments, and the period from resection of necrotic mandible to the closure of the fistula with D-P flap and reconstructive surgery for the mouth were also considered.

  18. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation

    DEFF Research Database (Denmark)

    Helin, K; Wu, C L; Fattaey, A R

    1993-01-01

    the hypophosphorylated form of the retinoblastoma protein (pRB). The other protein, murine DP-1, was purified from an E2F DNA-affinity column, and it was subsequently shown to bind the consensus E2F DNA-binding site. To study a possible interaction between E2F-1 and DP-1, we have now isolated a cDNA for the human...

  19. Silica-based PLC with heterogeneously-integrated PDs for one-chip DP-QPSK receiver.

    Science.gov (United States)

    Kurata, Yu; Nasu, Yusuke; Tamura, Munehisa; Kasahara, Ryoichi; Aozasa, Shinichi; Mizuno, Takayuki; Yokoyama, Haruki; Tsunashima, Satoshi; Muramoto, Yoshifumi

    2012-12-10

    To realize a DP-QPSK receiver PLC, we heterogeneously integrated eight high-speed PDs on a silica-based PLC platform with a PBS, 90-degree optical hybrids and a VOA. The use of a 2.5%-Δ waveguide reduced the receiver PLC size to 11 mm x 11 mm. We successfully demonstrated 32 Gbaud DP-QPSK signal demodulation with the receiver PLC.

  20. Wavelength conversion of a 128 Gbit/s DP-QPSK signal in a silicon polarization diversity circuit

    DEFF Research Database (Denmark)

    Vukovic, Dragana; Schroeder, Jochen; Ding, Yunhong

    2014-01-01

    Wavelength conversion of a 128 Gbit/s DP-QPSK signal is demonstrated using FWM in a polarization diversity circuit with silicon nanowires as nonlinear elements. Error-free performances are achieved with a negligible power penalty.......Wavelength conversion of a 128 Gbit/s DP-QPSK signal is demonstrated using FWM in a polarization diversity circuit with silicon nanowires as nonlinear elements. Error-free performances are achieved with a negligible power penalty....

  1. Correlation between 2D and 3D flow curve modelling of DP steels using a microstructure-based RVE approach

    International Nuclear Information System (INIS)

    Ramazani, A.; Mukherjee, K.; Quade, H.; Prahl, U.; Bleck, W.

    2013-01-01

    A microstructure-based approach by means of representative volume elements (RVEs) is employed to evaluate the flow curve of DP steels using virtual tensile tests. Microstructures with different martensite fractions and morphologies are studied in two- and three-dimensional approaches. Micro sections of DP microstructures with various amounts of martensite have been converted to 2D RVEs, while 3D RVEs were constructed statistically with randomly distributed phases. A dislocation-based model is used to describe the flow curve of each ferrite and martensite phase separately as a function of carbon partitioning and microstructural features. Numerical tensile tests of RVE were carried out using the ABAQUS/Standard code to predict the flow behaviour of DP steels. It is observed that 2D plane strain modelling gives an underpredicted flow curve for DP steels, while the 3D modelling gives a quantitatively reasonable description of flow curve in comparison to the experimental data. In this work, a von Mises stress correlation factor σ 3D /σ 2D has been identified to compare the predicted flow curves of these two dimensionalities showing a third order polynomial relation with respect to martensite fraction and a second order polynomial relation with respect to equivalent plastic strain, respectively. The quantification of this polynomial correlation factor is performed based on laboratory-annealed DP600 chemistry with varying martensite content and it is validated for industrially produced DP qualities with various chemistry, strength level and martensite fraction.

  2. Experiments and FE-simulations of stretch flanging of DP-steels with different shear cut edge quality

    Science.gov (United States)

    Sigvant, M.; Falk, J.; Pilthammar, J.

    2017-09-01

    Dual-Phase (DP) steels are today used in the automotive industry due to its large strength to weight ratio. However, the high strength of DP-steel does have a negative impact on the general formability in sheet metal forming. Unfavourable process conditions in the press shop will, on top of this, reduce the formability of DP-steels even more. This paper addresses the problem of edge fracture in stretch flanges in sheet metal parts made of DP-steel. The experimental part involves tests of ten different DP590 and DP780 steel grades with three different shear cut qualities. The influence on the fracture strain of the sample orientation of the shear cut are also studied by facing the burr away or towards the punch and testing samples with the cut edge parallel with the rolling direction and the transverse direction. The strains are measured with an ARAMIS system in each test, together with punch displacement and punch force. All tests are then simulated with AutoFormplus R7 and the results from these simulations are compared with the experimental results in order to find the appropriate failure strain for each combination of supplier, coating, thickness and shear cut quality.

  3. Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS and the phosphoinositide 3-kinase (PI3K/protein kinase B (Akt pathway

    Directory of Open Access Journals (Sweden)

    Svetlana Pavlovic

    2018-03-01

    Full Text Available Ketamine is a widely used anesthetic in pediatric clinical practice. Previous studies have demonstrated that ketamine induces neurotoxicity and has a modulatory effect on the cells of the immune system. Here, we evaluated the potential protective effect and underlying mechanisms of natural phenolic compound curcumin against ketamine-induced toxicity in rat thymocytes. Rat thymocytes were exposed to 100 µM ketamine alone or combined with increasing concentrations of curcumin (0.3, 1, and 3 μM for 24 hours. Cell viability was analyzed with CCK-8 assay kit. Apoptosis was analyzed using flow cytometry and propidium iodide as well as Z-VAD-FMK and Z-LEHD-FMK inhibitors. Reactive oxygen species (ROS production and mitochondrial membrane potential [MMP] were measured by flow cytometry. Colorimetric assay with DEVD-pNA substrate was used for assessing caspase-3 activity. Involvement of phosphoinositide 3-kinase (PI3K/protein kinase B (Akt signaling pathway was tested with Wortmannin inhibitor. Ketamine induced toxicity in cells, increased the number of hypodiploid cells, caspase-3 activity and ROS production, and inhibited the MMP. Co-incubation of higher concentrations of curcumin (1 and 3 μM with ketamine markedly decreased cytotoxicity, apoptosis rate, caspase-3 activity, and ROS production in rat thymocytes, and increased the MMP. Application of Z-VAD-FMK (a pan caspase inhibitor or Z-LEHD-FMK (caspase-9 inhibitor with ketamine effectively attenuated the ketamine-induced apoptosis in rat thymocytes. Administration of Wortmannin (a PI3K inhibitor with curcumin and ketamine significantly decreased the protective effect of curcumin on rat thymocytes. Our results indicate that ketamine-induced toxicity in rat thymocytes mainly occurs through the mitochondria-mediated apoptotic pathway and that the PI3K/Akt signaling pathway is involved in the anti-apoptotic effect of curcumin.

  4. Los Alamos DP West Plutonium Facility decontamination project, 1978-1981

    International Nuclear Information System (INIS)

    Garde, R.; Cox, E.J.; Valentine, A.M.

    1982-09-01

    The DP West Plutonium Facility operated by the Los Alamos National Laboratory, Los Alamos, New Mexico was decontaminated between April 1978 and April 1981. The facility was constructed in 1944 to 1945 to produce plutonium metal and fabricate parts for nuclear weapons. It was continually used as a plutonium processing and research facility until mid-1978. Decontamination operations included dismantling and removing gloveboxes and conveyor tunnels; removing process systems, utilities, and exhaust ducts; and decontaminating all remaining surfaces. This report describes glovebox and conveyor tunnel separations, decontamination techniques, health and safety considerations, waste management procedures, and costs of the operation

  5. Ductile Tearing Resistance Indexing of Automotive Grade DP 590 Steel Sheets: EWF Testing Using DENT Specimens

    Science.gov (United States)

    Sahoo, Subhadra; Padmapriya, N.; De, Partha Sarathi; Chakraborti, P. C.; Ray, S. K.

    2018-03-01

    The essential work of fracture (EWF) method has been explored for indexing the ductile tearing resistance of DP 590 automotive grade dual-phase steel sheet both in longitudinal (L-T) and transverse (T-L) orientations. The simplest possible test and analysis procedures have been adopted. The EWF method is found to be eminently suitable for routine quality control and product development purposes for such materials. Areas for further research for improving the experimental strategy are highlighted. For the investigated steel sheet, the estimated tearing resistance is found to be distinctly higher for the L-T orientation compared to the T-L orientation; the reason thereof merits further investigation.

  6. Notes on quantitative structure-properties relationships (QSPR) (1): A discussion on a QSPR dimensionality paradox (QSPR DP) and its quantum resolution.

    Science.gov (United States)

    Carbó-Dorca, Ramon; Gallegos, Ana; Sánchez, Angel J

    2009-05-01

    Classical quantitative structure-properties relationship (QSPR) statistical techniques unavoidably present an inherent paradoxical computational context. They rely on the definition of a Gram matrix in descriptor spaces, which is used afterwards to reduce the original dimension via several possible kinds of algebraic manipulations. From there, effective models for the computation of unknown properties of known molecular structures are obtained. However, the reduced descriptor dimension causes linear dependence within the set of discrete vector molecular representations, leading to positive semi-definite Gram matrices in molecular spaces. To resolve this QSPR dimensionality paradox (QSPR DP) here is proposed to adopt as starting point the quantum QSPR (QQSPR) computational framework perspective, where density functions act as infinite dimensional descriptors. The fundamental QQSPR equation, deduced from employing quantum expectation value numerical evaluation, can be approximately solved in order to obtain models exempt of the QSPR DP. The substitution of the quantum similarity matrix by an empirical Gram matrix in molecular spaces, build up with the original non manipulated discrete molecular descriptor vectors, permits to obtain classical QSPR models with the same characteristics as in QQSPR, that is: possessing a certain degree of causality and explicitly independent of the descriptor dimension. 2008 Wiley Periodicals, Inc.

  7. Evaluate and Analysis Efficiency of Safaga Port Using DEA-CCR, BCC and SBM Models-Comparison with DP World Sokhna

    Science.gov (United States)

    Elsayed, Ayman; Shabaan Khalil, Nabil

    2017-10-01

    The competition among maritime ports is increasing continuously; the main purpose of Safaga port is to become the best option for companies to carry out their trading activities, particularly importing and exporting The main objective of this research is to evaluate and analyze factors that may significantly affect the levels of Safaga port efficiency in Egypt (particularly the infrastructural capacity). The assessment of such efficiency is a task that must play an important role in the management of Safaga port in order to improve the possibility of development and success in commercial activities. Drawing on Data Envelopment Analysis(DEA)models, this paper develops a manner of assessing the comparative efficiency of Safaga port in Egypt during the study period 2004-2013. Previous research for port efficiencies measurement usually using radial DEA models (DEA-CCR), (DEA-BCC), but not using non radial DEA model. The research applying radial - output oriented (DEA-CCR), (DEA-BCC) and non-radial (DEA-SBM) model with ten inputs and four outputs. The results were obtained from the analysis input and output variables based on DEA-CCR, DEA-BCC and SBM models, by software Max DEA Pro 6.3. DP World Sokhna port higher efficiency for all outputs were compared to Safaga port. DP World Sokhna position is below the southern entrance to the Suez Canal, on the Red Sea, Egypt, makes it strategically located to handle cargo transiting through one of the world's busiest commercial waterways.

  8. Apoptosis and survival parameters during protection from radiation-induced thymocyte death by a candidate radioprotector, GC-2112, from Allium sativum

    International Nuclear Information System (INIS)

    Bunagan, J.; Perey, K.; Deocaris, C.C.

    1996-01-01

    Biomedical studies on nuclear fallout effects show that whole-body exposure to relatively low doses of ionizing radiation (2-10 Gy) induces the hematopoietic syndrome (HS) characterized by severe anemia and immunodeficiency and death within 10-30 days. The thymocyte model applies in many cell death researches and is found to undergo a morphologically and molecularly distinct p53-based apoptosis with DNA-damaging insults, such as radiation exposure. We have shown that exogenously applied radioprotector from allium sativum (garlic), GC-2112, improves total cellular survival for various observation periods concomitantly shifting the LD 50/24 from 7 Gy (control) to 21 Gy (GC-2112). This increased survival characteristic of the radioprotected macrophage-free thymocytes, however, fails to correlate with the prevention of apoptosis-associated DNA scissions. Mechanisms to the observed radiomodification may possibly involve cysteine compounds found rich in garlic. These preliminary findings show promise in the applications of selected herbal drugs as dietary prophylaxis against clinical morbidities arising from either medical, occupational or environmental exposures to ionizing radiation. (author)

  9. Requirement of mitoses for the reversal of X-inactivation in cell hybrids between murine embryonal carcinoma cells and normal female thymocytes

    Energy Technology Data Exchange (ETDEWEB)

    Takagi, N. (Hokkaido Univ., Sapporo (Japan))

    1988-04-01

    By means of a 5-bromodeoxyuridine (BrdU) incorporation and acridine orange fluorescence staining method the authors studied reactivation of the inactivated X chromosome (X{sub i}) in newly formed cell hybrids between the near-diploid HPRT-deficient OTF9-63 murine embryonal carcinoma cell (ECC) with an XO sex chromosome constitution and the normal female mouse thymocyte. Synchronization of the late replicating S chromosome in such hybrid cells, indicative of reactivation, was found for the first time on Day 3, and the frequency of reactivation was attained 90% on Day 5. Inhibition of cell cycle progression either by methylglyoxal bis(guanylhydrazone) dihydrochloride, an inhibitor of polyamine metabolism, or by isoleucine-deficient medium after cell fusion delayed reactivation of the X{sub i}, which implied that the number of cell division cycles traversed by individual cells rather than the length of time after cell fusion is critical for the reactivation. Double-labeling experiments using ({sup 3}H)thymidine and BrdU indicated that hybrid cells had undergone three or four mitoses before reactivation of the X{sub i}. Most probably reactivation of the X{sub i} is consequent to reversion of the thymocyte genome to an undifferentiated state under the influence of OTF9 genome. DNA demethylation or dilution of X{sub i}-specific factors by mitoses may be involved in this process.

  10. Requirement of mitoses for the reversal of X-inactivation in cell hybrids between murine embryonal carcinoma cells and normal female thymocytes

    International Nuclear Information System (INIS)

    Takagi, N.

    1988-01-01

    By means of a 5-bromodeoxyuridine (BrdU) incorporation and acridine orange fluorescence staining method the authors studied reactivation of the inactivated X chromosome (X i ) in newly formed cell hybrids between the near-diploid HPRT-deficient OTF9-63 murine embryonal carcinoma cell (ECC) with an XO sex chromosome constitution and the normal female mouse thymocyte. Synchronization of the late replicating S chromosome in such hybrid cells, indicative of reactivation, was found for the first time on Day 3, and the frequency of reactivation was attained 90% on Day 5. Inhibition of cell cycle progression either by methylglyoxal bis(guanylhydrazone) dihydrochloride, an inhibitor of polyamine metabolism, or by isoleucine-deficient medium after cell fusion delayed reactivation of the X i , which implied that the number of cell division cycles traversed by individual cells rather than the length of time after cell fusion is critical for the reactivation. Double-labeling experiments using [ 3 H]thymidine and BrdU indicated that hybrid cells had undergone three or four mitoses before reactivation of the X i . Most probably reactivation of the X i is consequent to reversion of the thymocyte genome to an undifferentiated state under the influence of OTF9 genome. DNA demethylation or dilution of X i -specific factors by mitoses may be involved in this process

  11. Apoptosis and survival parameters during protection from radiation-induced thymocyte death by a candidate radioprotector, GC-2112, from Allium sativum

    Energy Technology Data Exchange (ETDEWEB)

    Bunagan, J; Perey, K [Pamantasan ng Lungsod ng Maynila, Manila (Philippines); Deocaris, C C [Philippine Nuclear Research Inst., Diliman, Quezon City (Philippines)

    1997-12-31

    Biomedical studies on nuclear fallout effects show that whole-body exposure to relatively low doses of ionizing radiation (2-10 Gy) induces the hematopoietic syndrome (HS) characterized by severe anemia and immunodeficiency and death within 10-30 days. The thymocyte model applies in many cell death researches and is found to undergo a morphologically and molecularly distinct p53-based apoptosis with DNA-damaging insults, such as radiation exposure. We have shown that exogenously applied radioprotector from allium sativum (garlic), GC-2112, improves total cellular survival for various observation periods concomitantly shifting the LD{sub 50/24} from 7 Gy (control) to 21 Gy (GC-2112). This increased survival characteristic of the radioprotected macrophage-free thymocytes, however, fails to correlate with the prevention of apoptosis-associated DNA scissions. Mechanisms to the observed radiomodification may possibly involve cysteine compounds found rich in garlic. These preliminary findings show promise in the applications of selected herbal drugs as dietary prophylaxis against clinical morbidities arising from either medical, occupational or environmental exposures to ionizing radiation. (author).

  12. Analysis of the Interaction of Dp44mT with Human Serum Albumin and Calf Thymus DNA Using Molecular Docking and Spectroscopic Techniques

    Directory of Open Access Journals (Sweden)

    Zhongjie Xu

    2016-06-01

    Full Text Available Di-2-pyridylketone-4,4,-dimethyl-3-thiosemicarbazone (Dp44mT exhibits significant antitumor activity. However, the mechanism of its pharmacological interaction with human serum albumin (HSA and DNA remains poorly understood. Here, we aimed to elucidate the interactions of Dp44mT with HSA and DNA using MTT assays, spectroscopic methods, and molecular docking analysis. Our results indicated that addition of HSA at a ratio of 1:1 did not alter the cytotoxicity of Dp44mT, but did affect the cytotoxicity of the Dp44mT-Cu complex. Data from fluorescence quenching and UV-VIS absorbance measurements demonstrated that Dp44mT could bind to HSA with a moderate affinity (Ka = approximately 104 M−1. CD spectra revealed that Dp44mT could slightly disrupt the secondary structure of HSA. Dp44mT could also interact with Ct-DNA, but had a moderate binding constant (KEB = approximately 104 M−1. Docking studies indicated that the IB site of HSA, but not the IIA and IIIA sites, could be favorable for Dp44mT and that binding of Dp44mT to HSA involved hydrogen bonds and hydrophobic force, consistent with thermodynamic results from spectral investigations. Thus, the moderate binding affinity of Dp44mT with HSA and DNA partially contributed to its antitumor activity and may be preferable in drug design approaches.

  13. Identification of hip surface arthroplasty failures with TcSC/TcmDP radionuclide imaging

    International Nuclear Information System (INIS)

    Thomas, B.J.; Amstutz, H.C.; Mai, L.L.; Webber, M.M.

    1982-01-01

    The roentgenographic identification of femoral component loosening after hip surface arthroplasty is often impossible because the metallic femoral component obscures the bone-cement interface. The use of combined technetium sulfur colloid and technetium methylene diphosphonate radionuclide imaging has been especially useful in the diagnosis of loosening. In 40 patients, follow-up combined TcSC and TcmDP scans at an average of three, nine, and 27 months postoperation revealed significant differences in the isotope uptakes in patients who had loose prostheses compared with those without complications. Scans were evaluated by first dividing them into eight anatomical regions and then rating the uptake in each region or 'zone' on a five-point scale. Results were compared using the Student's t-test and differences were noted between normal controls and patients who had femoral component loosening. Combining both TcSC and TcmDP studies increased the statistical significance obtained when comparing patients who had complications to those in the control group

  14. Fused-filament 3D printing (3DP) for fabrication of tablets.

    Science.gov (United States)

    Goyanes, Alvaro; Buanz, Asma B M; Basit, Abdul W; Gaisford, Simon

    2014-12-10

    The use of fused-filament 3D printing (FF 3DP) to fabricate individual tablets is demonstrated. The technology permits the manufacture of tablets containing drug doses tailored to individual patients, or to fabrication of tablets with specific drug-release profiles. Commercially produced polyvinyl alcohol (PVA) filament was loaded with a model drug (fluorescein) by swelling of the polymer in ethanolic drug solution. A final drug-loading of 0.29% w/w was achieved. Tablets of PVA/fluorescein (10 mm diameter) were printed using a 3D printer. It was found that changing the degree of infill percentage in the printer software varied the weight and volume of the printed tablets. The tablets were mechanically strong and no significant thermal degradation of the active occurred during printing. Dissolution tests were conducted in modified Hank's buffer. The results showed release profiles were dependent on the infill percentage used to print the tablet. The study indicates that FF 3DP has the potential to offer a new solution for fabricating personalized-dose medicines or unit dosage forms with controlled-release profiles. In addition, the low cost of FDM printers means the paradigm of extemporaneous or point-of-use manufacture of personalized-dose tablets is both feasible and attainable. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. Association of HLA-DP/DQ and STAT4 polymorphisms with ankylosing spondylitis in Southwest China.

    Science.gov (United States)

    Liu, Xinle; Yang, Bin; Li, Lixin; Cai, Bei; Liao, Yun; Li, Linhui; Wu, Zhiqiang; Wang, Lanlan

    2016-10-01

    Ankylosing spondylitis (AS) is a highly heritable complex inflammatory arthritis disease. Genetic factors are thought to be crucial in the pathogenesis of AS. However, few data are available on the relationship between HLA-DP/DQ and STAT4 polymorphisms and AS susceptibility in the Chinese population. Therefore, we examined HLA-DP/DQ and STAT4 polymorphisms (rs3077, rs9277535, rs7453920 and rs7574865) in a total of 779 subjects, including 400 AS and 379 age- and sex-matched healthy controls in Chinese. No significant difference was observed between AS patients and healthy controls in the allele frequency of rs3077, rs9277535 and rs7574865. However, there was a significant association between the HLA-DQ rs7453920 G/A variant and AS patients, with minor allele A correlated with a reduced risk of AS (allelic frequency, adjusted OR=0.66, 95% CI=0.55-0.78, p=4.0E-06; dominant model, adjusted OR=0.75, 95% CI=0.66-0.85, p=1.1E-05). Moreover, the haplotypes block AAA and GGA in the HLA gene significantly correlated with reduced risk of AS. This is the first study demonstrating the significant associations of SNP rs7453920 and the haplotypes in the HLA gene with the risk of AS in Southwest Chinese population. This research sheds new light on the significant relationship between HLA polymorphisms and AS. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. The molecular origin of the thiamine diphosphate-induced spectral bands of ThDP-dependent enzymes.

    Science.gov (United States)

    Kovina, Marina V; De Kok, Aart; Sevostyanova, Irina A; Khailova, Ludmila S; Belkina, Natalya V; Kochetov, German A

    2004-08-01

    New and previously published data on a variety of ThDP-dependent enzymes such as baker's yeast transketolase, yeast pyruvate decarboxylase and pyruvate dehydrogenase from pigeon breast muscle, bovine heart, bovine kidney, Neisseria meningitidis and E. coli show their spectral sensitivity to ThDP binding. Although ThDP-induced spectral changes are different for different enzymes, their universal origin is suggested as being caused by the intrinsic absorption of the pyrimidine ring of ThDP, bound in different tautomeric forms with different enzymes. Non-enzymatic models with pyrimidine-like compounds indicate that the specific protein environment of the aminopyrimidine ring of ThDP determines its tautomeric form and therefore the changeable features of the inducible effect. A polar environment causes the prevalence of the aminopyrimidine tautomeric form (short wavelength region is affected). For stabilization of the iminopyrimidine tautomeric form (both short- and long-wavelength regions are affected) two factors appear essential: (i) a nonpolar environment and (ii) a conservative carboxyl group of a specific glutamate residue interacting with the N1' atom of the aminopyrimidine ring. The two types of optical effect depend in a different way upon the pH, in full accordance with the hypothesis tested. From these studies it is concluded that the inducible optical rotation results from interaction of the aminopyrimidine ring with its asymmetric environment and is defined by the protonation state of N1' and the 4'-nitrogen. Copyright 2004 Wiley-Liss, Inc.

  17. Experiment list: SRX099379 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 66262,95.2,8.3,424 GSM803107: input DNA Ikaros knockout chromatin source_name=mouse Ik knockout DP thymocyte...s || genetic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes || chip

  18. Hybrid opto-digital signal processing in 112 Gbit/s DP-16QAM and DP-QDB transmission for long-haul large-Aeff pure-silica-core fiber links

    DEFF Research Database (Denmark)

    Asif, Rameez; Ahmad, Ramshah; Basir, Rabeea

    2016-01-01

    By means of numerical simulations, we demonstrated that all-optical signal processing methods (XPM-suppressor module and in-line nonlinear equalization) significantly increase the system performance of digital nonlinear compensation (digital backward propagation) and improve the system performanc...... in five-channel 112 Gbit/s DP-16QAM and DP-QDB transmission over 2400 km large- effective-area pure-silica-core fiber ((Formula presented.)-PSCF). The system performance is quantified with the help of Q-factor (dB) for both dispersion-managed and nondispersion-managed fiber links....

  19. 100-Gb/s InP DP-IQ modulator for small-form-factor pluggable coherent transceivers

    Science.gov (United States)

    Kikuchi, Nobuhiro; Ogiso, Yoshihiro; Yamada, Eiichi

    2016-02-01

    We developed a compact InP-based DP-IQ modulator for small-form-factor pluggable coherent transceivers. The modulator achieves 112-Gb/s DP-QPSK modulation with a driving voltage of 6 Vppd. In addition, it provides 86-Gb/s DP-16 QAM signal generation and 240-km transmission with negligible degradation of BER performance. The halfwavelength voltage of our recent device is 1.9 V, and a high median extinction ratio of over 32 dB was achieved for more than 1,400 child MZ modulators. We have also proposed an athermal InP-based twin IQ modulator that enables us to use a modulator in a TEC-free operation. It contributes to lowering the power consumption of transceivers. Under a constant driving condition, there is little change in 56-Gb/s x 2 QPSK modulation characteristics in the range of 20 to 80°C.

  20. Adaptation of D-P flap to the oro-facial fistula induced by radio-osteomyelitis of mandible

    International Nuclear Information System (INIS)

    Yamamoto, Etsuhide; Genba, Ryo-ichi; Hayatsu, Yoshikazu; Sunakawa, Hajime; Kohama, Gen-iku

    1980-01-01

    Intraoral partial resection of the mandible was performed on 3 patients with radiation-induced ostomyelitis and ostonecrosis of the mandible, and inflammation in the mandible disappeared. Residual oro-facial fistula was closed with D-P lap, and a good result was obtained. Treatments of radiation osteopathy, the time of the treatments, and the period from resection of necrotic mandible to the closure of the fistula with D-P lap and reconstructive surgery for the mouth were also considered. (Tsunoda, M.)

  1. Review of Sandia National Laboratories - Albuquerque New Mexico DOE/DP Critical Skills Development Progrmas FY04.

    Energy Technology Data Exchange (ETDEWEB)

    Gorman, Anna K; Wilson, Dominique; CLARK, KATHERINE

    2005-09-01

    Sandia National Laboratories has developed a portfolio of programs to address the critical skills needs of the DP labs, as identified by the 1999 Chiles Commission Report. The goals are to attract and retain the best and the brightest students and transition them into Sandia - and DP Complex - employees. The US Department of Energy/Defense Programs University Partnerships funded ten laboratory critical skills development programs in FY04. This report provides a qualitative and quantitative evaluation of these programs and their status. 3

  2. Experimental evaluation of prefiltering for 56 Gbaud DP-QPSK signal transmission in 75 GHz WDM grid

    DEFF Research Database (Denmark)

    Borkowski, Robert; de Carvalho, Luis Henrique H.; Silva, Edson Porto da

    2014-01-01

    We investigate optical prefiltering for 56Gbaud (224Gbit/s) electrical time-division multiplexed (ETDM) dual polarization (DP) quaternary phase shift keying (QPSK) transmission. Different transmitter-side optical filter shapes are tested and their bandwidths are varied. Comparison of studied filter...... shapes shows an advantage of a pre-emphasis filter. Subsequently, we perform a fiber transmission of the 56Gbaud DP QPSK signal filtered with the 65GHz pre-emphasis filter to fit the 75GHz transmission grid. Bit error rate (BER) of the signal remains below forward error correction (FEC) limit after 300km...

  3. Characterization and modelling techniques for gas metal arc welding of DP 600 sheet steels

    Energy Technology Data Exchange (ETDEWEB)

    Mukherjee, K.; Prahl, U.; Bleck, W. [RWTH Aachen University, Department of Ferrous Metallurgy (IEHK) (Germany); Reisgen, U.; Schleser, M.; Abdurakhmanov, A. [RWTH Aachen University, Welding and Joining Institute (ISF) (Germany)

    2010-11-15

    The objectives of the present work are to characterize the Gas Metal Arc Welding process of DP 600 sheet steel and to summarize the modelling techniques. The time-temperature evolution during the welding cycle was measured experimentally and modelled with the softwaretool SimWeld. To model the phase transformations during the welding cycle dilatometer tests were done to quantify the parameters for phase field modelling by MICRESS {sup registered}. The important input parameters are interface mobility, nucleation density, etc. A contribution was made to include austenite to bainite transformation in MICRESS {sup registered}. This is useful to predict the microstructure in the fast cooling segments. The phase transformation model is capable to predict the microstructure along the heating and cooling cycles of welding. Tensile tests have shown the evidence of failure at the heat affected zone, which has the ferrite-tempered martensite microstructure. (orig.)

  4. Quantifying the effects of tempering on individual phase properties of DP980 steel with nanoindentation

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, G. [Physical and Computational Sciences Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA (United States); Zhang, F. [School of Mechanical and Materials Engineering, Washington State University, Pullman, WA (United States); Ruimi, A. [Department of Mechanical Engineering, Texas A& M University, Doha (Qatar); Field, D.P. [School of Mechanical and Materials Engineering, Washington State University, Pullman, WA (United States); Sun, X., E-mail: xin.sun@pnnl.gov [Physical and Computational Sciences Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA (United States)

    2016-06-14

    Tempering treatment is conducted on a commercial dual phase (DP) 980 steel at 250 °C and 400 °C for 60 min each. Ferrite and martensite grains are distinguished using electron backscatter diffraction (EBSD) and scanning probe microscopy (SPM), and the martensite volume fractions (MVF) are determined based on the image quality (IQ) map. Indentation tests combined with a newly developed inverse method are used to obtain the individual phase flow properties in each sample. The results show that, i) tempering significantly reduces martensite yield strength, while it slightly reduces the ferrite yield strength; ii) tempering temperature has a more significant influence on the work hardening exponent of ferrite than that of martensite. As a validation, a simple rule-of-mixtures is used to verify the above-predicted individual phase flow stresses with the experimentally obtained overall true stress vs. true strain curves.

  5. Experimental Evaluation of Impairments in Unrepeatered DP-16QAM Link with Distributed Raman Amplification

    Directory of Open Access Journals (Sweden)

    Xiaodan Pang

    2017-03-01

    Full Text Available The transmission impairments of a Raman amplified link using dual-polarization 16-quadrature amplitude modulation (DP-16QAM are experimentally characterized. The impact of amplitude and phase noise on the signal due to relative intensity noise (RIN transfer from the pump are compared for two pumping configurations: first-order backward pumping and bi-directional pumping. Experimental results indicate that with increased Raman backward pump power, though the optical signal-to-noise ratio (OSNR is increased, so is the pump-induced amplitude and phase noise. The transmission performance is firstly improved by the enhanced OSNR at a low pump power until an optimum point is reached, and then the impairments due to pump-induced noise start to dominate. However, the introduction of a low pump power in the forward direction can further improve the system’s performance.

  6. Computation On dP Type power System Stabilizer Using Fuzzy Logic

    International Nuclear Information System (INIS)

    Iskandar, M.A.; Irwan, R.; Husdi; Riza; Mardhana, E.; Triputranto, A.

    1997-01-01

    Power system stabilizers (PSS) are widely applied in power generators to damp power oscillation caused by certain disturbances in order to increase the power supply capacity. PSS design is often suffered from the difficulty on setting periodically its parameters, which are gain and compensators, in order to have an optimal damping characteristic. This paper proposes a methode to determine parameters of dP type PSS by implementing fuzzy logic rules in a computer program,to obtain the appropriate characteristics of synchronous torque and damping torque. PSS with the calculated parameters is investigated on a simulation using a non-linear electric power system of a thermal generator connected to infinite bus system model. Simulation results show that great improvement in damping characteristic and enhancement of stability margin of electric power system are obtained by using the proposed PSS

  7. Numerical-experimental investigation of load paths in DP800 dual phase steel during Nakajima test

    Science.gov (United States)

    Bergs, Thomas; Nick, Matthias; Feuerhack, Andreas; Trauth, Daniel; Klocke, Fritz

    2018-05-01

    Fuel efficiency requirements demand lightweight construction of vehicle body parts. The usage of advanced high strength steels permits a reduction of sheet thickness while still maintaining the overall strength required for crash safety. However, damage, internal defects (voids, inclusions, micro fractures), microstructural defects (varying grain size distribution, precipitates on grain boundaries, anisotropy) and surface defects (micro fractures, grooves) act as a concentration point for stress and consequently as an initiation point for failure both during deep drawing and in service. Considering damage evolution in the design of car body deep drawing processes allows for a further reduction in material usage and therefore body weight. Preliminary research has shown that a modification of load paths in forming processes can help mitigate the effects of damage on the material. This paper investigates the load paths in Nakajima tests of a DP800 dual phase steel to research damage in deep drawing processes. Investigation is done via a finite element model using experimentally validated material data for a DP800 dual phase steel. Numerical simulation allows for the investigation of load paths with respect to stress states, strain rates and temperature evolution, which cannot be easily observed in physical experiments. Stress triaxiality and the Lode parameter are used to describe the stress states. Their evolution during the Nakajima tests serves as an indicator for damage evolution. The large variety of sheet metal forming specific load paths in Nakajima tests allows a comprehensive evaluation of damage for deep drawing. The results of the numerical simulation conducted in this project and further physical experiments will later be used to calibrate a damage model for simulation of deep drawing processes.

  8. Contribution to the study of low-energy (d,p) reactions on light nuclei; Contribution a l'etude des reactions (d,p) a basse energie sur noyaux legers

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen Van, S. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1967-05-15

    This work is carried out with a view to analysing low energy (d,p) reactions on light nuclei measured at Grenoble or Algiers and suggesting a direct mechanism. The distorted wave approximation has been applied; this requires the development of programmes for the IBM 7044 computer at the 'Institut des Mathematiques Appliquees' in Grenoble. The (d,p) reactions on {sup 14}N, {sup 9}Be, {sup 22}Ne and {sup 16}O are dealt with. A calculation of the interference of the direct interaction and of resonating effects has been applied to {sup 16}O. (author) [French] Ce travail a pour but d'analyser les reactions (d,p) a basse energie sur noyaux legers, mesurees a Grenoble ou a Alger et qui suggerent un mecanisme direct. L'approximation des ondes distordues a ete appliquee, necessitant la mise au point de programmes sur l'ordinateur IBM 7044 de l'Institut des Mathematiques Appliquees de Grenoble. Des reactions (d,p) sur {sup 14}N, {sup 9}Be, {sup 22}Ne et {sup 16}O sont traitees. Un calcul de l'interference de l'interaction directe et des effets resonnants a ete applique a {sup 16}O. (auteur)

  9. Contribution to the study of low-energy (d,p) reactions on light nuclei; Contribution a l'etude des reactions (d,p) a basse energie sur noyaux legers

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen Van, S [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1967-05-15

    This work is carried out with a view to analysing low energy (d,p) reactions on light nuclei measured at Grenoble or Algiers and suggesting a direct mechanism. The distorted wave approximation has been applied; this requires the development of programmes for the IBM 7044 computer at the 'Institut des Mathematiques Appliquees' in Grenoble. The (d,p) reactions on {sup 14}N, {sup 9}Be, {sup 22}Ne and {sup 16}O are dealt with. A calculation of the interference of the direct interaction and of resonating effects has been applied to {sup 16}O. (author) [French] Ce travail a pour but d'analyser les reactions (d,p) a basse energie sur noyaux legers, mesurees a Grenoble ou a Alger et qui suggerent un mecanisme direct. L'approximation des ondes distordues a ete appliquee, necessitant la mise au point de programmes sur l'ordinateur IBM 7044 de l'Institut des Mathematiques Appliquees de Grenoble. Des reactions (d,p) sur {sup 14}N, {sup 9}Be, {sup 22}Ne et {sup 16}O sont traitees. Un calcul de l'interference de l'interaction directe et des effets resonnants a ete applique a {sup 16}O. (auteur)

  10. Sequence analyses reveal that a TPR-DP module, surrounded by recombinable flanking introns, could be at the origin of eukaryotic Hop and Hip TPR-DP domains and prokaryotic GerD proteins.

    Science.gov (United States)

    Hernández Torres, Jorge; Papandreou, Nikolaos; Chomilier, Jacques

    2009-05-01

    The co-chaperone Hop [heat shock protein (HSP) organising protein] is known to bind both Hsp70 and Hsp90. Hop comprises three repeats of a tetratricopeptide repeat (TPR) domain, each consisting of three TPR motifs. The first and last TPR domains are followed by a domain containing several dipeptide (DP) repeats called the DP domain. These analyses suggest that the hop genes result from successive recombination events of an ancestral TPR-DP module. From a hydrophobic cluster analysis of homologous Hop protein sequences derived from gene families, we can postulate that shifts in the open reading frames are at the origin of the present sequences. Moreover, these shifts can be related to the presence or absence of biological function. We propose to extend the family of Hop co-chaperons into the kingdom of bacteria, as several structurally related genes have been identified by hydrophobic cluster analysis. We also provide evidence of common structural characteristics between hop and hip genes, suggesting a shared precursor of ancestral TPR-DP domains.

  11. Sequence analyses reveal that a TPR–DP module, surrounded by recombinable flanking introns, could be at the origin of eukaryotic Hop and Hip TPR–DP domains and prokaryotic GerD proteins

    Science.gov (United States)

    Papandreou, Nikolaos; Chomilier, Jacques

    2008-01-01

    The co-chaperone Hop [heat shock protein (HSP) organising protein] is known to bind both Hsp70 and Hsp90. Hop comprises three repeats of a tetratricopeptide repeat (TPR) domain, each consisting of three TPR motifs. The first and last TPR domains are followed by a domain containing several dipeptide (DP) repeats called the DP domain. These analyses suggest that the hop genes result from successive recombination events of an ancestral TPR–DP module. From a hydrophobic cluster analysis of homologous Hop protein sequences derived from gene families, we can postulate that shifts in the open reading frames are at the origin of the present sequences. Moreover, these shifts can be related to the presence or absence of biological function. We propose to extend the family of Hop co-chaperons into the kingdom of bacteria, as several structurally related genes have been identified by hydrophobic cluster analysis. We also provide evidence of common structural characteristics between hop and hip genes, suggesting a shared precursor of ancestral TPR–DP domains. Electronic supplementary material The online version of this article (doi:10.1007/s12192-008-0083-8) contains supplementary material, which is available to authorized users. PMID:18987995

  12. KARAKTERISASI MALTODEKSTRIN DP 3-9 SERTA KAJIAN POTENSI PENGGUNAANNYA SEBAGAI SUMBER KARBOHIDRAT PADA MINUMAN OLAHRAGA [Characterization of Maltodextrin DP 3-9 and Assesment of Its Potential Application as Carbohydrate Source in Sport Drink

    Directory of Open Access Journals (Sweden)

    Beni Hidayat 1

    2003-04-01

    Full Text Available This research was aimed at characterization of maltodextrin DP 3-9 (produced by enzymatic hydrolysis and membrane separation process as compared to commercial maltodextrin and glucose and assessment of its potential application as carbohydrate source in sport drink. The research showed that application of maltodextrin DP 3-9 had some advantages as compared to glucose with regard to absorption rate that was 2 times longer (60 minutes instead of 30 minutes, osmolality degree that was 5,6 times lower (178 mOsmol/kg as compared to 1000 mOsmol/kg, and relative sweetness degree that was 10 - 11 times lower (6,15-7,20 as compared to 57,00-61,00. However, thie application of maltodekstrin DP 3-9 had limitation which was shown by its viscosity characteristic that was 5,70 -- 6,20% higher (1,29 cSt and 1,37 cSt as compared to 1,22 cSt and 1,29 cSt. When compared to commercial maltodextrin, maltodextrin DP 3-9 is favorable as carbohydrate source in sport drink based on its absorption rate that was more than 2 times faster (60 minutes as compared to more than 120 minutes and storage stability at refrigeration temperature (which was more than 8 weeks, with or without sterilization; with sterilization.

  13. Exposure of tumor-bearing mice to extremely high-frequency electromagnetic radiation modifies the composition of fatty acids in thymocytes and tumor tissue.

    Science.gov (United States)

    Gapeyev, Andrew B; Kulagina, Tatiana P; Aripovsky, Alexander V

    2013-08-01

    To test the participation of fatty acids (FA) in antitumor effects of extremely high-frequency electromagnetic radiation (EHF EMR), the changes in the FA composition in the thymus, liver, blood plasma, muscle tissue, and tumor tissue in mice with Ehrlich solid carcinoma exposed to EHF EMR were studied. Normal and tumor-bearing mice were exposed to EHF EMR with effective parameters (42.2 GHz, 0.1 mW/cm2, 20 min daily during five consecutive days beginning the first day after the inoculation of tumor cells). Fatty acid composition of various organs and tissues of mice were determined using a gas chromatography. It was shown that the exposure of normal mice to EHF EMR or tumor growth significantly increased the content of monounsaturated FA (MUFA) and decreased the content of polyunsaturated FA (PUFA) in all tissues examined. Exposure of tumor-bearing mice to EHF EMR led to the recovery of FA composition in thymocytes to the state that is typical for normal animals. In other tissues of tumor-bearing mice, the exposure to EHF EMR did not induce considerable changes that would be significantly distinguished between disturbances caused by EHF EMR exposure or tumor growth separately. In tumor tissue which is characterized by elevated level of MUFA, the exposure to EHF EMR significantly decreased the summary content of MUFA and increased the summary content of PUFA. The recovery of the FA composition in thymocytes and the modification of the FA composition in the tumor under the influence of EHF EMR on tumor-bearing animals may have crucial importance for elucidating the mechanisms of antitumor effects of the electromagnetic radiation.

  14. Phenotypic and functional properties of murine thymocytes. II. Quantitation of host- and donor-derived cytolytic T lymphocyte precursors in regenerating radiation bone marrow chimeras

    International Nuclear Information System (INIS)

    Ceredig, R.; McDonald, H.R.

    1982-01-01

    Thymocytes from radiation bone marrow chimeras, in which donor bone marrow and irradiated recipient differed at the Thy-1 locus, were stained by indirect immunofluorescence with monoclonal anti-Thy-1 antibodies and analyzed by flow microfluorometry (FMF). Kinetic studies indicated an early appearance of host-derived (CBA, Thy-1.2 + ) thymocytes, which reaches maximum number of 10 to 20 x 10 6 cells at 12 to 16 days after bone marrow reconstitution. Donor-derived (AKR, Thy-1.1 + ) cells were not detectable until 10 to 12 days after reconstitution; subsequently, they increased exponentially in number until 28 days, when they accounted for essentially all cells in the thymus (50 x 10 6 ). Concomitant with the appearance and disappearance of host-derived cells was a change in their Thy-1 surface phenotype. In particular, the proportion of host cells having a ''mature'' phenotype (weakly Thy-1.2 staining) increased progressively with time after irradiation. Functional studies using a sensitive mixed leukocyte microculture system to quantitate cytolytic T lymphocyte precursors (CTL-P) were also carried out in regenerating chimeric thymuses. Initially, the regenerating thymus contained few CTL-P, but by 4 wk after reconstitution, frequencies similar to control adult thymuses were obtained. Analysis of the CTL-P content of host and donor-derived subpopulations, separated either by appropriate anti-Thy-1 antibody plus complement or by direct cell sorting, indicated that both host- and donor-derived cells contained appreciable numbers of CTL-P. Furthermore, increases in CTL-P frequency of both host and donor subpopulations correlated with changes in their surface Thy-1 phenotype

  15. Phenotypic and functional properties of murine thymocytes. II. Quantitation of host- and donor-derived cytolytic T lymphocyte precursors in regenerating radiation bone marrow chimeras

    Energy Technology Data Exchange (ETDEWEB)

    Ceredig, R.; McDonald, H.R.

    1982-02-01

    Thymocytes from radiation bone marrow chimeras, in which donor bone marrow and irradiated recipient differed at the Thy-1 locus, were stained by indirect immunofluorescence with monoclonal anti-Thy-1 antibodies and analyzed by flow microfluorometry (FMF). Kinetic studies indicated an early appearance of host-derived (CBA, Thy-1.2/sup +/) thymocytes, which reaches maximum number of 10 to 20 x 10/sup 6/ cells at 12 to 16 days after bone marrow reconstitution. Donor-derived (AKR, Thy-1.1/sup +/) cells were not detectable until 10 to 12 days after reconstitution; subsequently, they increased exponentially in number until 28 days, when they accounted for essentially all cells in the thymus (50 x 10/sup 6/). Concomitant with the appearance and disappearance of host-derived cells was a change in their Thy-1 surface phenotype. In particular, the proportion of host cells having a ''mature'' phenotype (weakly Thy-1.2 staining) increased progressively with time after irradiation. Functional studies using a sensitive mixed leukocyte microculture system to quantitate cytolytic T lymphocyte precursors (CTL-P) were also carried out in regenerating chimeric thymuses. Initially, the regenerating thymus contained few CTL-P, but by 4 wk after reconstitution, frequencies similar to control adult thymuses were obtained. Analysis of the CTL-P content of host and donor-derived subpopulations, separated either by appropriate anti-Thy-1 antibody plus complement or by direct cell sorting, indicated that both host- and donor-derived cells contained appreciable numbers of CTL-P. Furthermore, increases in CTL-P frequency of both host and donor subpopulations correlated with changes in their surface Thy-1 phenotype.

  16. Expression of antigens coded in murine leukemia viruses on thymocytes of allogeneic donor origin in AKR mice following syngeneic or allogeneic bone marrow transplantation

    International Nuclear Information System (INIS)

    Wustrow, T.P.; Good, R.A.

    1985-01-01

    Removal of T-lymphocytes from marrow inoculum with monoclonal antibody plus complement permitted establishment of long-lived allogeneic chimeras between C57BL/6 and AKR/J mice. Development of leukemia was prevented for 15 mo. Protection from leukemia occurred with both young (4 wk) and older (4 mo) recipients. AKR mice reconstituted with syngeneic marrow or control AKR mice all developed leukemia-lymphoma before 1 yr of age. During spontaneous lymphomagenesis in AKR mice, amplified expression of gag or env gene-coded virus antigens on the surface of thymocytes preceded leukemia development and evidence for amplification of other virus genes. These changes generally appeared before 6 mo. Similar viral gene expression and viral gene amplification occurred in the thymus and spleen cells of leukemia-resistant chimeric mice. Using monoclonal antibodies to Mr 70,000 glycoprotein epitopes characteristic of ecotropic, xenotropic, or dualtropic viruses, antigens marking each virus form were found on thymocytes of allogeneic 4-wk and 4-mo chimeras as well as on the cells of AKR mice and of AKR mice reconstituted with syngeneic marrow. Flow cytometric analysis showed amplification of the virus genes in mice protected from leukemia-lymphoma by allogeneic bone marrow transplantation from leukemia-resistant mice. Allogeneic chimeras and syngeneically transplanted mice both showed evidence of accelerated viremia and of recombinant virus formation. The findings suggest that an event essential to leukemogenesis which occurs within the AKR lymphoid cells or their environment is lacking in the allogeneic chimeras. The nature of this influence of a resistance gene or genes introduced into AKR mice by allogeneic bone marrow transplantation deserves further study

  17. Association of HLA-DP/DQ, STAT4 and IL-28B variants with HBV viral clearance in Tibetans and Uygurs in China.

    Science.gov (United States)

    Liao, Yun; Cai, Bei; Li, Yi; Chen, Jie; Ying, Binwu; Tao, Chuanmin; Zhao, Min; Ba, Zhu; Zhang, Zhaoxia; Wang, Lanlan

    2015-03-01

    Several genome-wide association studies have revealed that HLA-DP/DQ, STAT4 and IL-28B associated with liver diseases. But because of population heterogeneity, different races would have different causative polymorphisms. Therefore, in this study, we included Chinese Tibetans and Uygurs to examine the roles of these genes on HBV natural clearance. A total of 1341 subjects including 908 Tibetans and 433 Uygurs were recruited. Seven single nucleotide polymorphisms (SNP) were genotyped. HLA-DP/DQ polymorphisms associated with HBV natural clearance in both ethnicities (Tibetans, rs3077, OR = 0.61, 95% CI = 0.46-0.82; rs9277535, OR = 0.56, 95% CI = 0.41-0.75; rs7453920, OR = 0.64, 95%CI = 0.47-0.85; Uygurs, rs3077, OR = 0.48, 95% CI = 0.24-0.96; rs9277535, OR = 0.43, 95% CI = 0.20-0.91; rs7453920, OR = 0.62, 95% CI = 0.39-0.99), whereas no significant association was observed between IL-28B with HBV natural clearance in neither ethnicities (P > 0.05). STAT4 rs7574865 seemed to be Tibetan specific in HBV natural clearance (OR = 0.76, 95% CI = 0.58-0.99). Moreover, in Tibetan patients, HLA-DQ rs7453920 GG had a higher frequency in HBeAg positive patients (P = 0.032) and STAT4 rs7574865 GG genotype appeared more frequently in Genotype C virus infected patients (P = 0.005). In addition, Uygurs have higher frequencies of HLA-DP/DQ protective alleles (72.5% for rs3077, 76.6% for rs9277535 and 26.8% for rs7453920) than Tibetans (51.7% for rs3077, 52.5% for rs9277535 and 18.5% for rs7453920)(all P rs7574865 seemed to be population specific in Tibetans and it might synergistically interact with virus contributing to disease progression. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  18. Microstructural evolution and mechanical performance of resistance spot welded DP1000 steel with single and double pulse welding

    NARCIS (Netherlands)

    Chabok, Ali; van der Aa, Ellen; De Hosson, Jeff; Pei, Yutao T.

    2017-01-01

    Two welding schemes of single and double pulse were used for the resistance spot welding of DP1000 dual phase steel. The changes in the mechanical performance and variant pairing of martensite under two different welding conditions were scrutinized. It is demonstrated that, although both welds fail

  19. Molecular Dynamics Simulations of a Cyclic DP-240 Amylose Fragment in a Periodic Cell: Glass Transition Temperature and Water Diffusion

    Science.gov (United States)

    Molecular dynamics simulations using AMB06C, an in-house carbohydrate force field, (NPT ensembles, 1atm) were carried out on a periodic cell that contained a cyclic-DP-240 amylose fragment and TIP3P water molecules. Molecular conformation and movement of the amylose fragment and water molecules at ...

  20. How Peripheral a Phenomenon Is It?: On the Interaction of DP-Internal Ellipsis, Degree Inversion, and Islands

    Science.gov (United States)

    Manlove, Kathleen

    2016-01-01

    In this dissertation I set out to solve a series of puzzles related to the notion of a DP periphery, defined as an area around the edge of a given domain targeted by operations such as movement and agreement. In solving these puzzles, I argue for a peripheral area in the nominal domain. Early arguments for a peripheral boundary in the nominal…

  1. Measurements of T20 in backward elastic dp scattering at deuteron momenta 3.5--6 GeV/c

    International Nuclear Information System (INIS)

    Azhgirey, L.S.; Borzunov, Y.T.; Chernykh, E.V.; Golovanov, L.B.; Ignatenko, M.A.; Kobushkin, A.P.; Ladygin, V.P.; Manyakov, P.K.; Moroz, N.S.; Nedev, S.; Penchev, L.; Perdrisat, C.F.; Piskunov, N.M.; Punjabi, V.; Rukoyatkin, P.A.; Sitnik, I.M.; Stoletov, G.D.; Strokovsky, E.A.; Svetov, A.L.; Syamtomov, A.I.; Tsvinev, A.P.; Vikhrov, V.V.; Vizireva, L.; Zaporozhets, S.A.

    1995-01-01

    Polarization phenomena in backward elastic dp scattering were studied with a view to reconstructing the deuteron wave-function components. Experimental data disagree with predictions based on the impulse approximation which suggests that the wave function may have more than two components. (AIP) copyright 1995 American Institute of Physics

  2. CD4+CD8β+ double-positive T cells in skin-draining lymph nodes respond to inflammatory signals from the skin

    DEFF Research Database (Denmark)

    Overgaard, Nana Haahr; Cruz, Jazmina L.; Bridge, Jennifer A.

    2017-01-01

    CD4+CD8+ double-positive (DP), mature, peripheral T cells are readily detectable in a variety of species and tissues. Despite a common association with autoimmune and malignant skin disorders, however, little is understood about their role or function. Herein, we show that DP T cells are readily ...

  3. DP97, a DEAD box DNA/RNA helicase, is a target gene-selective co-regulator of the constitutive androstane receptor

    International Nuclear Information System (INIS)

    Kanno, Yuichiro; Serikawa, Takafumi; Inajima, Jun; Inouye, Yoshio

    2012-01-01

    Highlights: ► DP97 interacts with nuclear receptor CAR. ► DP97 enhances CAR-mediated transcriptional activation. ► DP97 synergistically enhances transactivity of CAR by the co-expression of SRC-1 or PGC1α. ► DP97 is a gene-selective co-activator for hCAR. -- Abstract: The constitutive androstane receptor (CAR) plays a key role in the expression of xenobiotic/steroid and drug metabolizing enzymes and their transporters. In this study, we demonstrated that DP97, a member of the DEAD box DNA/RNA helicase protein family, is a novel CAR-interacting protein. Using HepG2 cells expressing human CAR in the presence of tetracycline, we showed that knockdown of DP97 with small interfering RNAs suppressed tetracycline-inducible mRNA expression of CYP2B6 and UGT1A1 but not CYP3A4. Thus, DP97 was found to be a gene (or promoter)-selective co-activator for hCAR. DP97-mediated CAR transactivation was synergistically enhanced by the co-expression of SRC-1 or PGC1α, therefore it might act as mediator between hCAR and appropriate co-activators.

  4. DP97, a DEAD box DNA/RNA helicase, is a target gene-selective co-regulator of the constitutive androstane receptor

    Energy Technology Data Exchange (ETDEWEB)

    Kanno, Yuichiro, E-mail: ykanno@phar.toho-u.ac.jp [Faculty of Pharmaceutical Sciences, Toho University, Chiba (Japan); Serikawa, Takafumi; Inajima, Jun; Inouye, Yoshio [Faculty of Pharmaceutical Sciences, Toho University, Chiba (Japan)

    2012-09-14

    Highlights: Black-Right-Pointing-Pointer DP97 interacts with nuclear receptor CAR. Black-Right-Pointing-Pointer DP97 enhances CAR-mediated transcriptional activation. Black-Right-Pointing-Pointer DP97 synergistically enhances transactivity of CAR by the co-expression of SRC-1 or PGC1{alpha}. Black-Right-Pointing-Pointer DP97 is a gene-selective co-activator for hCAR. -- Abstract: The constitutive androstane receptor (CAR) plays a key role in the expression of xenobiotic/steroid and drug metabolizing enzymes and their transporters. In this study, we demonstrated that DP97, a member of the DEAD box DNA/RNA helicase protein family, is a novel CAR-interacting protein. Using HepG2 cells expressing human CAR in the presence of tetracycline, we showed that knockdown of DP97 with small interfering RNAs suppressed tetracycline-inducible mRNA expression of CYP2B6 and UGT1A1 but not CYP3A4. Thus, DP97 was found to be a gene (or promoter)-selective co-activator for hCAR. DP97-mediated CAR transactivation was synergistically enhanced by the co-expression of SRC-1 or PGC1{alpha}, therefore it might act as mediator between hCAR and appropriate co-activators.

  5. Thermal conductivity of the cryoprotective cocktail DP6 in cryogenic temperatures, in the presence and absence of synthetic ice modulators.

    Science.gov (United States)

    Ehrlich, Lili E; Malen, Jonathan A; Rabin, Yoed

    2016-10-01

    The thermal conductivity of the cryoprotective agent (CPA) cocktail DP6 in combination with synthetic ice modulators (SIMs) is measured in this study, using a transient hot-wire method. DP6 is a mixture of 3 M dimethyl sulfoxide (DMSO) and 3 M propylene glycol, which received significant attention in the cryobiology community in recent years. Tested SIMs include 6% 1,3Cyclohexanediol, 6% 2,3Butanediol, and 12% PEG400 (percentage by volume). This study integrates the scanning cryomacroscope for visual verification of crystallization and vitrification events. It is demonstrated that the thermal conductivity of the vitrifying CPA cocktail decreases monotonically with the decreasing temperature down to -180 °C. By contrast, the thermal conductivity of the crystalline material increases with decreasing temperature in the same temperature range. Results of this study demonstrate that the thermal conductivity may vary by three fold between the amorphous and crystalline phases of DP6 below the glass transition temperature of DP6 (Tg = -119 °C). The selected SIMs demonstrate the ability to inhibit crystallization in DP6, even at subcritical cooling rates. An additional ice suppression capability is observed by the Euro-Collins as a vehicle solution, disproportionate to its volume ratio in the cocktail. The implication of the observed thermal conductivity differences between the amorphous and crystalline phases of the same cocktail on cryopreservation simulations is significant in some cases and must be taken into account in thermal analyses of cryopreservation protocols. Copyright © 2016. Published by Elsevier Inc.

  6. Identification and cloning of a prethymic precursor T lymphocyte from a population of common acute lymphoblastic leukemia antigen (CALLA)-positive fetal bone marrow cells

    DEFF Research Database (Denmark)

    Hokland, P; Hokland, M; Daley, J

    1987-01-01

    We have cloned common acute lymphoblastic leukemia (CALLA)-positive cells from human fetal bone marrow containing less than 1 in 10,000 E-RFC in round-bottomed microtiter wells (one cell per well) using the autocloning unit of an EPICS-V cell sorter. Expansion of such cells (with IL-2 and heavily...... irradiated autologous thymocytes as feeder cells) resulted in growth in 6-14% of the wells (mean, 11%) with cells with mature T lymphocyte phenotype. Two-color fluorescence analysis of outgrowing cultures furthermore ascertained that these cells had differentiated through a phase of simultaneous expression...... of T4 and T8 antigens and at the same time expression of the thymocyte-associated T6 antigens. Thus, given the fact that 10-20% of T cell acute lymphoblastic leukemia (T-ALLs) are CALLA+, we have been able to identify a human prethymic T lymphocyte population that might be the normal counterpart...

  7. Effect of process parameters on optimum welding condition of DP590 steel by friction stir welding

    International Nuclear Information System (INIS)

    Kim, Young Gon; Kim, Ji Sun; Kim, In Ju

    2014-01-01

    In the automotive industry, vehicle weight reduction techniques have been actively studied to improve the rate of fuel consumption and to cope with the regulation restricting exhaust gas. For this reason, advanced high-strength steel (AHSS) is preferred in the automobile industry as its tensile strength is 590 MPa and over. In this study, to obtain the optimum welding condition, the friction stir welding (FSW) process applied to AHSS was considered. The FSW experiment was performed on a stir plate using a Si 3 N 4 tool and a 1.4-mm thick DP590 steel sheet manufactured by cold rolling. In addition, to investigate the temperature distribution of the advancing and retreating sides in the welding state, the tool rotation speed of 800 rpm, and the welding speed of 180 mm/min, a K-type thermocouple was inserted in the backing plate, and the peak temperature was evaluated at each point. Especially, the correlation between the heat input per unit length and the formation of the FSW zone was minutely analyzed.

  8. Thermal Expansion of Vitrified Blood Vessels Permeated with DP6 and Synthetic Ice Modulators

    Science.gov (United States)

    Eisenberg, David P.; Taylor, Michael J.; Jimenez-Rios, Jorge L.; Rabin, Yoed

    2014-01-01

    This study provides thermal expansion data for blood vessels permeated with the cryoprotective cocktail DP6, when combined with selected synthetic ice modulators (SIMs): 12% polyethylene glycol 400, 6% 1,3-cyclohexanediol, and 6% 2,3-butanediol. The general classification of SIMs includes molecules that modulate ice nucleation and growth, or possess properties of stabilizing the amorphous state, by virtue of their chemical structure and at concentrations that are not explained on a purely colligative basis. The current study is part of an ongoing effort to characterize thermo-mechanical effects on structural integrity of cryopreserved materials, where thermal expansion is the driving mechanism to thermo-mechanical stress. This study focuses on the lower part of the cryogenic temperature range, where the cryoprotective agent (CPA) behaves as a solid for all practical applications. By combining results obtained in the current study with literature data on the thermal expansion in the upper part of the cryogenic temperature range, unified thermal expansion curves are presented. PMID:24769313

  9. Effect of process parameters on optimum welding condition of DP590 steel by friction stir welding

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young Gon; Kim, Ji Sun; Kim, In Ju [Korea Institute of Industrial Technology, Gwangju (Korea, Republic of)

    2014-12-15

    In the automotive industry, vehicle weight reduction techniques have been actively studied to improve the rate of fuel consumption and to cope with the regulation restricting exhaust gas. For this reason, advanced high-strength steel (AHSS) is preferred in the automobile industry as its tensile strength is 590 MPa and over. In this study, to obtain the optimum welding condition, the friction stir welding (FSW) process applied to AHSS was considered. The FSW experiment was performed on a stir plate using a Si{sub 3}N{sub 4} tool and a 1.4-mm thick DP590 steel sheet manufactured by cold rolling. In addition, to investigate the temperature distribution of the advancing and retreating sides in the welding state, the tool rotation speed of 800 rpm, and the welding speed of 180 mm/min, a K-type thermocouple was inserted in the backing plate, and the peak temperature was evaluated at each point. Especially, the correlation between the heat input per unit length and the formation of the FSW zone was minutely analyzed.

  10. On-Line High Dose-rate Gamma Irradiation Test of the Profibus/DP module

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Jai Wan; Choi, Young Soo; Kim, Chang Hoi; Koo, In Soo; Hong, Seok Boong [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2009-05-15

    The field bus data communication is considered for application in nuclear environments. The nuclear facilities, including nuclear power plants, high radioactivity waste disposals, reprocessing plants and thermonuclear fusion installations can benefit from the unique advantages of the field bus communication network for the smart field instruments and controls. A major problem which arises when dealing with one in these nuclear environments, in special circumstances such as the RCS (reactor coolant system) area, is the presence of high gamma-ray irradiation fields. Radioactive constraints for the DBA(design basis accident) qualification of the RTD transmitter installed in the inside of the RCS pump are typically on the order of 4kGy/h with total doses up to 10kGy. In order to use an industrial field bus communication network as an ad-hoc sensor data link in the vicinity of the RCS area of the nuclear power plant, the robust survivability of these system in such intense gamma-radiation fields therefore needs to be verified. We have conducted high dose-rate (up to 4kGy) gamma irradiation experiments on a profibus/DP communication module. In this paper we describe the evolution of its basic characteristics with high dose-rate gamma irradiation and shortly explain the observed phenomena.

  11. Development of ultralight, super-elastic, hierarchical metallic meta-structures with i3DP technology

    Science.gov (United States)

    Zhang, Dongxing; Xiao, Junfeng; Moorlag, Carolyn; Guo, Qiuquan; Yang, Jun

    2017-11-01

    Lightweight and mechanically robust materials show promising applications in thermal insulation, energy absorption, and battery catalyst supports. This study demonstrates an effective method for creation of ultralight metallic structures based on initiator-integrated 3D printing technology (i3DP), which provides a possible platform to design the materials with the best geometric parameters and desired mechanical performance. In this study, ultralight Ni foams with 3D interconnected hollow tubes were fabricated, consisting of hierarchical features spanning three scale orders ranging from submicron to centimeter. The resultant materials can achieve an ultralight density of as low as 5.1 mg cm-3 and nearly recover after significant compression up to 50%. Due to a high compression ratio, the hierarchical structure exhibits superior properties in terms of energy absorption and mechanical efficiency. The relationship of structural parameters and mechanical response was established. The ability of achieving ultralight density printing approach provides metallic structures with substantial benefits from the hierarchical design and fabrication flexibility to ultralight applications.

  12. The therapeutic potential of CRTH2/DP2 beyond allergy and asthma.

    Science.gov (United States)

    Jandl, Katharina; Heinemann, Akos

    2017-11-01

    Prostaglandin (PG) D 2 has been in the focus of research for quite a long time, but its biological effects and its roles in human disease are still not fully characterized. When in 2001 a second major PGD 2 receptor termed chemoattractant receptor homologue expressed on Th2 cells (CRTH2; alternative name DP2) was discovered, diverse investigations started to shed more light on the complex and often controversial actions of the prostaglandin. With various immunomodulating effects, such as induction of migration, activation, and cytokine release of leukocytes observed both in vivo and in vitro, CRTH2 has emerged as a promising target for the treatment of allergic diseases. However, with more and more research being performed on CRTH2, it has also become clear that its biological actions are far more diverse than expected at the beginning. In this review, we aim to summarize the roles that PGD 2 - and CRTH2 in particular - might play in diseases of the central nervous system, kidney, intestine, lung, hair and skin, bone and cartilage, and in cancer. Based on current data we propose that blocking CRTH2 might be a potential therapeutic approach to numerous conditions beyond classical allergic diseases and asthma. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  13. Microstructure and mechanical properties of a medium-carbon bainitic steel by a novel quenching and dynamic partitioning (Q-DP) process

    Energy Technology Data Exchange (ETDEWEB)

    Li, Qiangguo; Huang, Xuefei; Huang, Weigang, E-mail: huangwg56@163.com

    2016-04-26

    A novel Quenching and Dynamic Partitioning (Q-DP) process for a 0.3C-1.4Si-1.8Mn-1.3Cr-0.3Mo (wt%) bainitic steel was developed and the microstructure and mechanical properties were investigated. The results show that the microstructure of the Q-DP treated steel consists of bainite, martensite and retained austenite, and it exhibit a better combination of tensile strength (above 1500 MPa), total elongation (above 17%) and impact toughness (above 90 J). Among the different Q-DP process, the sample treated by 250 °C Q-DP process exhibits the best combination of strength (1519 MPa), ductility (21.3%), the product of strength and elongation (PSE, 32.4 GPa%) and maximum impact toughness (108 J) compared to the quenching and partitioning (Q&P) process and other Q-DP processes. In addition, the work hardening behaviors of the Q&P and Q-DP samples were investigated. The stress-strain curves show that the Q&P and 250 °C Q-DP treated samples exhibit the larger uniform elongation and the value of n calculated for samples is 0.109 and 0.101 respectively.

  14. Some new effects of the deuteron D state observed in (p,d) and (d,p) reactions

    International Nuclear Information System (INIS)

    Ohnuma, Hajime

    1980-01-01

    Two previously unexplored experiments have revealed the importance of the deuteron D-state effects on (p,d) and (d,p) reactions at moderate energies. Firstly, a clear indication of the deuteron D-state effects on the polarization of the residual nuclear state has been observed in the 58 Ni(p,dγ) angular correlation measurement at E sub(p) = 30 MeV. Secondly, a comparison of the vector analyzing power and vector polarization measured at E sub(d) = 22 MeV for an l = 0 (d,p) transition has shown that the D state has significant effects even on the first-rank polarization quantities. The experimental data and the results of exact-finite-range DWBA calculations with Reid soft-core potential are presented. (author)

  15. Computer-assisted study on the reaction between pyruvate and ylide in the pathway leading to lactyl-ThDP.

    Science.gov (United States)

    Alvarado, Omar; Jaña, Gonzalo; Delgado, Eduardo J

    2012-08-01

    In this study the formation of the lactyl-thiamin diphosphate intermediate (L-ThDP) is addressed using density functional theory calculations at X3LYP/6-31++G(d,p) level of theory. The study includes potential energy surface scans, transition state search, and intrinsic reaction coordinate calculations. Reactivity is analyzed in terms of Fukui functions. The results allow to conclude that the reaction leading to the formation of L-ThDP occurs via a concerted mechanism, and during the nucleophilic attack on the pyruvate molecule, the ylide is in its AP form. The calculated activation barrier for the reaction is 19.2 kcal/mol, in agreement with the experimental reported value.

  16. In-situ bending under tension shear fracture analysis and microstructure “earthquake” of DP780 dual phase steels

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Yixi, E-mail: yxzhao@sjtu.edu.cn [State Key Laboratory of Mechanical System and Vibration, Shanghai Key Laboratory of Digital Manufacture for Thin-walled Structures, Shanghai Jiao Tong University, Shanghai 200240 (China); Huang, Sheng [State Key Laboratory of Mechanical System and Vibration, Shanghai Key Laboratory of Digital Manufacture for Thin-walled Structures, Shanghai Jiao Tong University, Shanghai 200240 (China); Dan, Wenjiao; Zhang, Weigang [Innovation Center for Advanced Ship and Deep-Sea Exploration, Department of Engineering Mechanics, Shanghai Jiao Tong University, Shanghai 200240 (China); Li, Shuhui [State Key Laboratory of Mechanical System and Vibration, Shanghai Key Laboratory of Digital Manufacture for Thin-walled Structures, Shanghai Jiao Tong University, Shanghai 200240 (China)

    2017-05-17

    Dual phase (DP) steels consist of hard brittle martensite phase and soft ductile ferrite phase. With a novel bending under tension test system, in-situ symmetrical bending under tension experiments were carried out and photomicrographs of bending surface were recorded. The microstructure “earthquake” of DP780 dual phase steels was observed in the bending under tension process. By analyzing the in-situ images serious, the initiation, coalescence of cavities and propagation of micro-cracks until final fracture were analyzed. The micro-cracks form only in the outside surface of bending radius, and mainly appear near the phase boundary of ferrite and martensite. Micro-cracks coalesce and propagate in the direction perpendicular to the stretching direction approximately, and at the phase boundary of martensite and ferrite. Furthermore, digital image correlation technology was used in this study to analysis the strain distribution between ferrite and martensite during the bending under tension deformation and fracture.

  17. Study of the possibility to use dp-elastic scattering for the Nuclotron external deuteron beam polarimetry

    International Nuclear Information System (INIS)

    Gurchin, Yu.V.; Isupov, A.Yu.; Khrenov, A.N.; Kiselev, A.S.; Ladygin, V.P.; Reznikov, S.G.; Vasil'ev, T.A.; Janek, M.; Karachuk, J.T.

    2011-01-01

    A selection of dp-elastic scattering events at energies of 1.6 and 2.0 GeV by using scintillation counters has been performed. The procedure of the CH 2 -C subtraction has been established. The dependence of the elastic events yield on the filter thickness has been investigated. This method can be used to develop the efficient high-energy deuteron beam polarimetry

  18. Strain- and stress-based forming limit curves for DP 590 steel sheet using Marciniak-Kuczynski method

    Science.gov (United States)

    Kumar, Gautam; Maji, Kuntal

    2018-04-01

    This article deals with the prediction of strain-and stress-based forming limit curves for advanced high strength steel DP590 sheet using Marciniak-Kuczynski (M-K) method. Three yield criteria namely Von-Mises, Hill's 48 and Yld2000-2d and two hardening laws i.e., Hollomon power and Swift hardening laws were considered to predict the forming limit curves (FLCs) for DP590 steel sheet. The effects of imperfection factor and initial groove angle on prediction of FLC were also investigated. It was observed that the FLCs shifted upward with the increase of imperfection factor value. The initial groove angle was found to have significant effects on limit strains in the left side of FLC, and insignificant effect for the right side of FLC for certain range of strain paths. The limit strains were calculated at zero groove angle for the right side of FLC, and a critical groove angle was used for the left side of FLC. The numerically predicted FLCs considering the different combinations of yield criteria and hardening laws were compared with the published experimental results of FLCs for DP590 steel sheet. The FLC predicted using the combination of Yld2000-2d yield criterion and swift hardening law was in better coorelation with the experimental data. Stress based forming limit curves (SFLCs) were also calculated from the limiting strain values obtained by M-K model. Theoretically predicted SFLCs were compared with that obtained from the experimental forming limit strains. Stress based forming limit curves were seen to better represent the forming limits of DP590 steel sheet compared to that by strain-based forming limit curves.

  19. Microstructure and Fatigue Properties of Laser Welded DP590 Dual-Phase Steel Joints

    Science.gov (United States)

    Xie, Chaojie; Yang, Shanglei; Liu, Haobo; Zhang, Qi; Cao, Yaming; Wang, Yuan

    2017-08-01

    In this paper, cold-rolled DP590 dual-phase steel sheets with 1.5 mm thickness were butt-welded by a fiber laser, and the evolution and effect on microhardness, tensile property and fatigue property of the welded joint microstructure were studied. The results showed that the base metal is composed of ferrite and martensite, with the martensite dispersed in the ferrite matrix in an island manner. The microstructure of the weld zone was lath-shaped martensite that can be refined further by increasing the welding speed, while the heat-affected zone was composed of ferrite and tempered martensite. The microhardness increased with increasing welding speed, and the hardness reached its highest value—393.8 HV—when the welding speed was 5 m/min. Static tensile fracture of the welded joints always occurred in the base metal, and the elongation at break was more than 16%. The conditional fatigue limits of the base metal and the weld joints were 354.2 and 233.6 MPa, respectively, under tension-tension fatigue tests with a stress rate of 0.1. After observation of the fatigue fracture morphology, it was evident that the fatigue crack of the base metal had sprouted into the surface pits and that its expansion would be accelerated under the action of a secondary crack. The fatigue source of the welded joint was generated in the weld zone and expanded along the martensite, forming a large number of fatigue striations. Transient breaking, which occurred in the heat-affected zone of the joint as a result of the formation of a large number of dimples, reflected the obvious characteristics of ductile fracture.

  20. Destruction of DOE/DP surrogate wastes with supercritical water oxidation technology

    International Nuclear Information System (INIS)

    Bramlette, T.T.; Mills, B.E.; Hencken, K.R.; Brynildson, M.E.; Johnston, S.C.; Hruby, J.M.; Freemster, H.C.; Odegard, B.C.; Modell, M.

    1990-11-01

    Surrogate wastes of specific interest to DOE/DP production facilities (Hanford and Rocky Flats), and the electronics industry have been successfully processed in a laboratory-scale, supercritical water oxidation flow reactor. In all cases, the observed destruction/reduction efficiencies for the organic components were in excess of 99.9%, limited by instrumentation detection capability. Separation of the inorganic components of the Hanford process stream was more difficult to accomplish than destruction of the organic component. Large fractions of all metals contained in this stream were found both in the solids separator effluent and in deposits removed from the reactor. Mass closure was not achieved. Of the process stream's non-metallic, inorganic components, the sulfates and phosphates precipitated, while the nitrates tended to stay in solution. The inorganic material that did precipitate from the simulated Hanford mixed waste accumulated in zones that may be associated with changes in the chemical and physical properties of the supercritical fluid. Corrosion is expected to be a significant problem. Witness wires of Inconel 625, Hastalloy C-276, and titanium placed in the preheater, reactor and cooldown exchanger indicated selective dissolution of chromium, nickel, and molybdenum for some conditions, and non-selective dissolution for others. While these results are very promising, further research is required to evaluate the scalability, reliability, and economics of SCWO reactor components and systems, particularly for mixed wastes. Future research must explore a parameter space (temperature, pressure, pH, residence time, etc.) focused on selecting conditions and materials for specific process streams

  1. Effects of processing parameters on the friction stir spot joining of Al5083-O aluminum alloy to DP590 steel

    Science.gov (United States)

    Sung, Back-Sub; Bang, Hee-Seon; Jeong, Su-Ok; Choi, Woo-Seong; Kwon, Yong-Hyuk; Bang, Han-Sur

    2017-05-01

    Two dissimilar materials, aluminum alloy Al5083-O and advanced high strength steel DP590, were successfully joined by using friction stir spot joining (FSSJ). Satisfactory joint strengths were obtained at a rotational speed of 300 rpm and a plunge depth of 0.7 mm. Resulting joints were welded without a non-welded zone. This may be attributed to the enhanced smooth material flow owing to sufficient stirring effect and tool down force between the upper Al5083-O side and the lower DP590 side. The maximum tensile shear strength was 6.5 kN, which was higher than the joint strength required by the conventional method of resistance spot welding. The main fracture mode was plug fracture in the tensile shear test of joints. An intermetallic compound (IMC) layer with material Al-Fe joints. Thus, the use of FSSJ to weld the dissimilar materials Al5083-O and DP590 resulted in mechanically and metallurgically sound joints.

  2. Development and psychometric properties of a belief-based Physical Activity Questionnaire for Diabetic Patients (PAQ-DP).

    Science.gov (United States)

    Ghazanfari, Zeinab; Niknami, Shamsaddin; Ghofranipour, Fazlollah; Hajizadeh, Ebrahim; Montazeri, Ali

    2010-11-09

    This study carried out to develop a scale for assessing diabetic patients' perceptions about physical activity and to test its psychometric properties (The Physical Activity Questionnaire for Diabetic Patients-PAQ-DP). An item pool extracted from the Theory of Planned Behavior literature was generated. Then an expert panel evaluated the items by assessing content validity index and content validity ratio. Consequently exploratory factor analysis (EFA) was performed to indicate the scale constructs. In addition reliability analyses including internal consistency and test-retest analysis were carried out. In all a sample of 127 women with diabetes participated in the study. Twenty-two items were initially extracted from the literature. A six-factor solution (containing 19 items) emerged as a result of an exploratory factor analysis namely: instrumental attitude, subjective norm, perceived behavioral control, affective attitude, self-identity, and intention explaining 60.30% of the variance observed. Additional analyses indicated satisfactory results for internal consistency (Cronbach's alpha ranging from 0.54 to 0.8) and intraclass correlation coefficients (ranging from 0.40 to 0.92). The Physical Activity Questionnaire for Diabetic Patients (PAQ-DP) is the first instrument that applies the Theory of Planned Behavior in its constructs. The findings indicated that the PAQ-DP is a reliable and valid measure for assessing physical activity perceptions and now is available and can be used in future studies.

  3. Development and psychometric properties of a belief-based Physical Activity Questionnaire for Diabetic Patients (PAQ-DP

    Directory of Open Access Journals (Sweden)

    Ghofranipour Fazlollah

    2010-11-01

    Full Text Available Abstract Background This study carried out to develop a scale for assessing diabetic patients' perceptions about physical activity and to test its psychometric properties (The Physical Activity Questionnaire for Diabetic Patients-PAQ-DP. Methods An item pool extracted from the Theory of Planned Behavior literature was generated. Then an expert panel evaluated the items by assessing content validity index and content validity ratio. Consequently exploratory factor analysis (EFA was performed to indicate the scale constructs. In addition reliability analyses including internal consistency and test-retest analysis were carried out. Results In all a sample of 127 women with diabetes participated in the study. Twenty-two items were initially extracted from the literature. A six-factor solution (containing 19 items emerged as a result of an exploratory factor analysis namely: instrumental attitude, subjective norm, perceived behavioral control, affective attitude, self-identity, and intention explaining 60.30% of the variance observed. Additional analyses indicated satisfactory results for internal consistency (Cronbach's alpha ranging from 0.54 to 0.8 and intraclass correlation coefficients (ranging from 0.40 to 0.92. Conclusions The Physical Activity Questionnaire for Diabetic Patients (PAQ-DP is the first instrument that applies the Theory of Planned Behavior in its constructs. The findings indicated that the PAQ-DP is a reliable and valid measure for assessing physical activity perceptions and now is available and can be used in future studies.

  4. Individual or synchronous biodegradation of di-n-butyl phthalate and phenol by Rhodococcus ruber strain DP-2

    Energy Technology Data Exchange (ETDEWEB)

    He, Zhixing; Niu, Chengzhen; Lu, Zhenmei, E-mail: lzhenmei@zju.edu.cn

    2014-05-01

    Highlights: • A Rhodococcus ruber strain degraded DBP and phenol. • Degradation kinetics of DBP or phenol fit modified first-order models. • Degradation interaction between DBP and phenol was studied by strain DP-2. • The degradation genes transcriptional were quantified by RT-qPCR. - Abstract: The bacterial strain DP-2, identified as Rhodococcus ruber, is able to effectively degrade di-n-butyl phthalate (DBP) and phenol. Degradation kinetics of DBP and phenol at different initial concentrations revealed DBP and phenol degradation to fit modified first-order models. The half-life of DBP degradation ranged from 15.81 to 27.75 h and phenol degradation from 14.52 to 45.52 h under the initial concentrations of 600–1200 mg/L. When strain DP-2 was cultured with a mixture of DBP (800 mg/L) and phenol (700 mg/L), DBP degradation rate was found to be only slightly influenced; however, phthalic acid (PA) accumulated, and phenol degradation was clearly inhibited during synchronous degradation. Transcriptional levels of degradation genes, phenol hydroxylase (pheu) and phthalate 3,4-dioxygenase (pht), decreased significantly more during synchronous degradation than during individual degradation. Quantitative estimation of individual or synchronous degradation kinetics is essential to manage mixed hazardous compounds through biodegradation in industrial waste disposal.

  5. Study the bonding mechanism of binders on hydroxyapatite surface and mechanical properties for 3DP fabrication bone scaffolds.

    Science.gov (United States)

    Wei, Qinghua; Wang, Yanen; Li, Xinpei; Yang, Mingming; Chai, Weihong; Wang, Kai; zhang, Yingfeng

    2016-04-01

    In 3DP fabricating artificial bone scaffolds process, the interaction mechanism between binder and bioceramics power determines the microstructure and macro mechanical properties of Hydroxyapatite (HA) bone scaffold. In this study, we applied Molecular Dynamics (MD) methods to investigating the bonding mechanism and essence of binders on the HA crystallographic planes for 3DP fabrication bone scaffolds. The cohesive energy densities of binders and the binding energies, PCFs g(r), mechanical properties of binder/HA interaction models were analyzed through the MD simulation. Additionally, we prepared the HA bone scaffold specimens with different glues by 3DP additive manufacturing, and tested their mechanical properties by the electronic universal testing machine. The simulation results revealed that the relationship of the binding energies between binders and HA surface is consistent with the cohesive energy densities of binders, which is PAM/HA>PVA/HA>PVP/HA. The PCFs g(r) indicated that their interfacial interactions mainly attribute to the ionic bonds and hydrogen bonds which formed between the polar atoms, functional groups in binder polymer and the Ca, -OH in HA. The results of mechanical experiments verified the relationship of Young׳s modulus for three interaction models in simulation, which is PVA/HA>PAM/HA>PVP/HA. But the trend of compressive strength is PAM/HA>PVA/HA>PVP/HA, this is consistent with the binding energies of simulation. Therefore, the Young׳s modulus of bone scaffolds are limited by the Young׳s modulus of binders, and the compressive strength is mainly decided by the viscosity of binder. Finally, the major reasons for differences in mechanical properties between simulation and experiment were found, the space among HA pellets and the incomplete infiltration of glue were the main reasons influencing the mechanical properties of 3DP fabrication HA bone scaffolds. These results provide useful information in choosing binder for 3DP fabrication

  6. Dynamics of Lymphocyte Populations during Trypanosoma cruzi Infection: From Thymocyte Depletion to Differential Cell Expansion/Contraction in Peripheral Lymphoid Organs

    Directory of Open Access Journals (Sweden)

    Alexandre Morrot

    2012-01-01

    Full Text Available The comprehension of the immune responses in infectious diseases is crucial for developing novel therapeutic strategies. Here, we review current findings on the dynamics of lymphocyte subpopulations following experimental acute infection by Trypanosoma cruzi, the causative agent of Chagas disease. In the thymus, although the negative selection process of the T-cell repertoire remains operational, there is a massive thymocyte depletion and abnormal release of immature CD4+CD8+ cells to peripheral lymphoid organs, where they acquire an activated phenotype similar to activated effector or memory T cells. These cells apparently bypassed the negative selection process, and some of them are potentially autoimmune. In infected animals, an atrophy of mesenteric lymph nodes is also observed, in contrast with the lymphocyte expansion in spleen and subcutaneous lymph nodes, illustrating a complex and organ specific dynamics of lymphocyte subpopulations. Accordingly, T- and B-cell activation is seen in subcutaneous lymph nodes and spleen, but not in mesenteric lymph nodes. Lastly, although the function of peripheral CD4+CD8+ T-cell population remains to be defined in vivo, their presence may contribute to the immunopathological events found in both murine and human Chagas disease.

  7. Anti-thymocyte globulin could improve the outcome of allogeneic hematopoietic stem cell transplantation in patients with highly aggressive T-cell tumors

    International Nuclear Information System (INIS)

    Yang, J; Cai, Y; Jiang, J L; Wan, L P; Yan, S K; Wang, C

    2015-01-01

    The early experiment result in our hospital showed that anti-thymocyte globulin (ATG) inhibited the proliferation of lymphoid tumor cells in the T-cell tumors. We used the ATG as the part of the conditioning regimen and to evaluate the long-term anti-leukemia effect, the safety and complication in the patients with highly aggressive T-cell lymphomas. Twenty-three patients were enrolled into this study. At the time of transplant, six patients reached first or subsequent complete response, three patients had a partial remission and 14 patients had relapsed or primary refractory disease. The conditioning regimen consisted of ATG, total body irradiation, toposide and cyclophosphamide. The complete remission rate after transplant was 95.7%. At a median follow-up time of 25 months, 16 (69.6%) patients are alive and free from diseases, including nine patients in refractory and progressive disease. Seven patients died after transplant, five from relapse and two from treatment-related complications. The incidence of grades II–IV acute graft-vs-host disease (GvHD) was 39.1%. The maximum cumulative incidence of chronic GvHD was 30%. The most frequent and severe conditioning-related toxicities observed in 8 out of 23 patients were grades III/IV infections during cytopenia. Thus, ATG-based conditioning is a feasible and effective alternative for patients with highly aggressive T-cell tumors

  8. Bcl2-independent chromatin cleavage is a very early event during induction of apoptosis in mouse thymocytes after treatment with either dexamethasone or ionizing radiation.

    Science.gov (United States)

    Hahn, Peter J; Lai, Zhi-Wei; Nevaldine, Barbara; Schiff, Ninel; Fiore, Nancy C; Silverstone, Allen E

    2003-11-01

    We have quantified the emergence of early chromatin breaks during the signal transduction phase of apoptosis in mouse thymocytes after treatment with either ionizing radiation or dexamethasone. Dexamethasone at 1 microM can induce significant levels of DNA breaks (equivalent to the amount induced directly by 7.5 Gy ionizing radiation) within 0.5 h of treatment. The execution phase of apoptosis was not observed until 4-6 h after the same treatment. The presence of the Bcl2 transgene under the control of the p56lck promoter almost completely inhibited apoptosis up to 24 h after treatment, but it had virtually no effect on the early chromatin cleavage occurring in the first 6 h. Ionizing radiation induced chromatin cleavage both directly by damaging DNA and indirectly with kinetics similar to the induction of chromatin cleavage by dexamethasone. The presence of the Bcl2 transgene had no effect on the direct or indirect radiation-induced cleavage in the first 6 h, but after the first 6 h, the Bcl2 gene inhibited further radiation-induced chromatin cleavage. These results suggest that endonucleases are activated within minutes of treatment with either dexamethasone or ionizing radiation as part of the very early signal transduction phase of apoptosis, and prior to the irreversible commitment to cell death.

  9. The novel thiosemicarbazone, di-2-pyridylketone 4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC, inhibits neuroblastoma growth in vitro and in vivo via multiple mechanisms

    Directory of Open Access Journals (Sweden)

    Zhu-Ling Guo

    2016-09-01

    Full Text Available Abstract Background Neuroblastoma is a relatively common and highly belligerent childhood tumor with poor prognosis by current therapeutic approaches. A novel anti-cancer agent of the di-2-pyridylketone thiosemicarbazone series, namely di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT, demonstrates promising anti-tumor activity. Recently, a second-generation analogue, namely di-2-pyridylketone 4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC, has entered multi-center clinical trials for the treatment of advanced and resistant tumors. The current aim was to examine if these novel agents were effective against aggressive neuroblastoma in vitro and in vivo and to assess their mechanism of action. Methods Neuroblastoma cancer cells as well as immortalized normal cells were used to assess the efficacy and selectivity of DpC in vitro. An orthotopic SK-N-LP/Luciferase xenograft model was used in nude mice to assess the efficacy of DpC in vivo. Apoptosis in tumors was confirmed by Annexin V/PI flow cytometry and H&E staining. Results DpC demonstrated more potent cytotoxicity than Dp44mT against neuroblastoma cells in a dose- and time-dependent manner. DpC significantly increased levels of phosphorylated JNK, neuroglobin, cytoglobin, and cleaved caspase 3 and 9, while decreasing IkBα levels in vitro. The contribution of JNK, NF-ĸB, and caspase signaling/activity to the anti-tumor activity of DpC was verified by selective inhibitors of these pathways. After 3 weeks of treatment, tumor growth in mice was significantly (p < 0.05 reduced by DpC (4 mg/kg/day given intravenously and the agent was well tolerated. Xenograft tissues showed significantly higher expression of neuroglobin, cytoglobin, caspase 3, and tumor necrosis factor-α (TNFα levels and a slight decrease in interleukin-10 (IL-10. Conclusions DpC was found to be highly potent against neuroblastoma, demonstrating its potential as a novel therapeutic for this disease. The ability

  10. Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils

    OpenAIRE

    Wang, Yanhui; Li, Honghong; Feng, Guojun; Du, Liangwei; Zeng, Dongqiang

    2017-01-01

    A diuron-degrading endophyte DP8-1 was isolated from sugarcane root grown in diuron-treated soil in the present study. The endophyte was identified as Neurospora intermedia based on the morphological characteristics and sequence analysis. The fermentation parameters including temperature, pH, inoculation size, carbon source, and initial diuron concentration were also investigated for the optimization of degradation efficiency. The results indicated that strain DP8-1 was capable of degrading u...

  11. Bayesian Nonparametric Clustering for Positive Definite Matrices.

    Science.gov (United States)

    Cherian, Anoop; Morellas, Vassilios; Papanikolopoulos, Nikolaos

    2016-05-01

    Symmetric Positive Definite (SPD) matrices emerge as data descriptors in several applications of computer vision such as object tracking, texture recognition, and diffusion tensor imaging. Clustering these data matrices forms an integral part of these applications, for which soft-clustering algorithms (K-Means, expectation maximization, etc.) are generally used. As is well-known, these algorithms need the number of clusters to be specified, which is difficult when the dataset scales. To address this issue, we resort to the classical nonparametric Bayesian framework by modeling the data as a mixture model using the Dirichlet process (DP) prior. Since these matrices do not conform to the Euclidean geometry, rather belongs to a curved Riemannian manifold,existing DP models cannot be directly applied. Thus, in this paper, we propose a novel DP mixture model framework for SPD matrices. Using the log-determinant divergence as the underlying dissimilarity measure to compare these matrices, and further using the connection between this measure and the Wishart distribution, we derive a novel DPM model based on the Wishart-Inverse-Wishart conjugate pair. We apply this model to several applications in computer vision. Our experiments demonstrate that our model is scalable to the dataset size and at the same time achieves superior accuracy compared to several state-of-the-art parametric and nonparametric clustering algorithms.

  12. Demonstration of the serum antibody to Epstein-Barr virus specific DNA polymerased (EBV-DP) from patients with nasopharyngeal carcinoma (NPC)

    Energy Technology Data Exchange (ETDEWEB)

    Tan, R.S.; Li, J.S.; Grill, S.; Nutter, L.M.; Cheng, Y.C.

    1986-03-05

    Raji cells, an EBV genome carrying and nonproducer cell line, treated with tetradecanoyl-phorbol-13-acetate (TPA) and n-butyrate could induce a special DNA polymerase which has properties that are similar to the EBV-DP induced by TPA in P/sub 3/HR-I cells, an EBV producer cell line. Since EBV was found to have a strong association with NPC, and antibodies against EBV proteins or enzymes were found in high levels in sera from these patients, the possible presence of serum antibody against EBV-DP was examined. The serum titer of antibody to EBV-DP was found to have 190 +/- 84 units/ml serum (mean +/- S.D.) in 48 sera from patients with NPC. The titer in 52 healthy donors was 31.4 +/- 28 unit/ml serum (p < 0.01). The antibody was found to be associated with the IgG but not the IgA fraction. The antibody titers against EBV-DP were not correlated with the titer against EBV-DNase or VCA-IgA. Whether the antibody observed is against an EBV-DP core protein or its stimulating protein, as demonstrated by this laboratory previously, is still being investigated. This study demonstrated the high frequency and high titer of antibody against EBV-DP in serum from patients with NPC, and suggested the potential of utilizing this antibody titer to compliment other methods for the early diagnosis or prognosis of NPC.

  13. A Non-Destructive Optical Method for the DP Measurement of Paper Insulation Based on the Free Fibers in Transformer Oil

    Directory of Open Access Journals (Sweden)

    Lei Peng

    2018-03-01

    Full Text Available In order to explore a non-destructive method for measuring the polymerization degree (DP of paper insulation in transformer, a new method that based on the optical properties of free fiber particles in transformer oil was studied. The chromatic dispersion images of fibers with different aging degree were obtained by polarizing microscope, and the eigenvalues (r, b, and Mahalanobis distance of the images were extracted by the RGB (red, blue, and green tricolor analysis method. Then, the correlation between the three eigenvalues and DP of paper insulation were simulated respectively. The results showed that the color of images changed from blue-purple to orange-yellow gradually with the increase of aging degree. For the three eigenvalues, the relationship between Mahalanobis distance and DP had the best goodness of fit (R2 = 0.98, higher than that of r (0.94 and b (0.94. The mean square error of the relationship between Mahalanobis distance and DP (52.17 was also significantly lower than that of r and b (97.58, 98.05. Therefore, the DP of unknown paper insulation could be calculated by the simulated relationship of Mahalanobis distance and DP.

  14. Design and experiments with scale model of a ship with dynamic positioning system

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Carlos Eduardo S.; Morishita, Helio M.; Moratelli Junior, Lazaro; Lago, Glenan A.; Tannuri, Eduardo A. [Universidade de Sao Paulo (USP), SP (Brazil)

    2008-07-01

    Dynamic Positioning Systems (DPS) are used to keep a floating vessel on a specific position or follow pre-defined path through the action of controlled propellers. This paper describes a facility used to experimentally analyze DPS and to validate a numerical simulator. It is composed by a scale model of a DP tanker with 3 thrusters, a measurement system based on computational vision and a control software with the same DP algorithms used in industrial systems. Simple wind and current generators were also implemented. This work shows preliminary results of experiments, which has been useful to calibrate the simulator and to validate the mathematical model. (author)

  15. Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues.

    Science.gov (United States)

    Eisenberg, David P; Taylor, Michael J; Rabin, Yoed

    2012-10-01

    This study explores physical effects associated with the application of cryopreservation via vitrification using a class of compounds which are defined here as synthetic ice modulators (SIMs). The general classification of SIMs includes molecules that modulate ice nucleation and growth, or possess properties of stabilizing the amorphous state, by virtue of their chemical structure and at concentrations that are not explained on a purely colligative basis. A sub-category of SIMs, referred to in the literature as synthetic ice blockers (SIBs), are compounds that interact directly with ice nuclei or crystals to modify their structure and/or rate of growth. The current study is part of an ongoing effort to characterize thermo-mechanical effects during vitrification, with emphasis on measuring the physical property of thermal expansion-the driving mechanism to thermo-mechanical stress. Materials under investigation are the cryoprotective agent (CPA) cocktail DP6 in combination with one of the following SIMs: 12% polyethylene glycol 400, 6% 1,3 cyclohexanediol, and 6% 2,3 butanediol. Results are presented for the CPA-SIM cocktail in the absence and presence of bovine muscle and goat artery specimens. This study focuses on the upper part of the cryogenic temperature range, where the CPA behaves as a fluid for all practical applications. Results of this study indicate that the addition of SIMs to DP6 allows lower cooling rates to ensure vitrification and extends the range of measurements. It is demonstrated that the combination of SIM with DP6 increases the thermal expansion of the cocktail, with implications for the likelihood of fracture formation-the most dramatic outcome of thermo-mechanical stress. Copyright © 2012 Elsevier Inc. All rights reserved.

  16. Floodplain and Wetland Assessment for the Mortandad Wetland Enhancement and the DP Dissipater Projects at Los Alamos National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Hathcock, Charles Dean [Los Alamos National Laboratory

    2017-03-31

    This floodplain and wetland assessment was prepared in accordance with 10 Code of Federal Regulations (CFR) 1022 Compliance with Floodplain and Wetland Environmental Review Requirements, which was promulgated to implement the U.S. Department of Energy (DOE) requirements under Executive Order 11988 Floodplain Management and Executive Order 11990 Wetlands Protection. According to 10 CFR 1022, a 100-year floodplain is defined as “the lowlands adjoining inland and coastal waters and relatively flat areas and flood prone areas of offshore islands” and a wetland is defined as “an area that is inundated or saturated by surface or groundwater at a frequency and duration sufficient to support, and that under normal circumstances does support, a prevalence of vegetation typically adapted for life in saturated soil conditions, including swamps, marshes, bogs, and similar areas.” In this action, DOE is proposing two projects to improve wetland and floodplain function at Los Alamos National Laboratory (LANL). The proposed work will comply with corrective action requirements under the Settlement Agreement and Stipulated Final Compliance Order (Settlement Agreement)1 Number HWB-14-20. The first project is located in Technical Areas (TA)-03 in upper Mortandad Canyon. The upper Mortandad wetlands have existing stormwater controls that need to be rehabilitated. Head-cut formation is occurring at the downstream portion of the wetland. This project will repair damages to the wetland and reduce the future erosion potential. The second project is located in TA-21 in Delta Prime (DP) Canyon. The intent of the DP Dissipater Project in DP Canyon is to install stormwater control structures in DP Canyon to retain low channel flows and reduce downstream sediment transport as well as peak flows during low and moderate storm events. Due to increased erosion, the stream bank in this area has unstable vertical walls within the stream channel. The DOE prepared this floodplain and wetland

  17. Wake-promoting effects of ONO-4127Na, a prostaglandin DP1 receptor antagonist, in hypocretin/orexin deficient narcoleptic mice.

    Science.gov (United States)

    Sagawa, Yohei; Sato, Masatoshi; Sakai, Noriaki; Chikahisa, Sachiko; Chiba, Shintaro; Maruyama, Takashi; Yamamoto, Junki; Nishino, Seiji

    2016-11-01

    Prostaglandin (PG)D2 is an endogenous sleep substance, and a series of animal studies reported that PGD2 or PGD2 receptor (DP1) agonists promote sleep, while DP1 antagonists promote wakefulness. This suggests the possibility of use of PG DP1 antagonists as wake-promoting compounds. We therefore evaluated the wake-promoting effects of ONO-4127Na, a DP1 antagonist, in a mouse model of narcolepsy (i.e., orexin/ataxin-3 transgenic mice) and compared those to effects of modafinil. ONO-4127Na perfused in the basal forebrain (BF) area potently promoted wakefulness in both wild type and narcoleptic mice, and the wake-promoting effects of ONO-4127Na at 2.93 × 10(-4) M roughly corresponded to those of modafinil at 100 mg/kg (p.o.). The wake promoting effects of ONO-4127Na was observed both during light and dark periods, and much larger effects were seen during the light period when mice slept most of the time. ONO-4127Na, when perfused in the hypothalamic area, had no effects on sleep. We further demonstrated that wake-promoting effects of ONO-4127Na were abolished in DP1 KO mice, confirming that the wake-promoting effect of ONO-4127Na is mediated by blockade of the PG DP1 receptors located in the BF area. ONO-4127Na reduced DREM, an EEG/EMG assessment of behavioral cataplexy in narcoleptic mice, suggesting that ONO-4127Na is likely to have anticataplectic effects. DP1 antagonists may be a new class of compounds for the treatment of narcolepsy-cataplexy, and further studies are warranted. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Micro–macro-characterisation and modelling of mechanical properties of gas metal arc welded (GMAW) DP600 steel

    Energy Technology Data Exchange (ETDEWEB)

    Ramazani, A., E-mail: ali.ramazani@iehk.rwth-aachen.de [Department of Ferrous Metallurgy, RWTH Aachen University, D-52072 Aachen (Germany); Mukherjee, K. [Department of Ferrous Metallurgy, RWTH Aachen University, D-52072 Aachen (Germany); Abdurakhmanov, A. [Welding and Joining Institute, RWTH Aachen University, D-52072 Aachen (Germany); Prahl, U. [Department of Ferrous Metallurgy, RWTH Aachen University, D-52072 Aachen (Germany); Schleser, M.; Reisgen, U. [Welding and Joining Institute, RWTH Aachen University, D-52072 Aachen (Germany); Bleck, W. [Department of Ferrous Metallurgy, RWTH Aachen University, D-52072 Aachen (Germany)

    2014-01-01

    Dual-phase (DP) steels show combined high strength and adequate formability. However, during welding, their microstructural feature of dispersion of hard martensite islands in the soft ferrite matrix is lost and the properties deteriorate. The current research aims to study the mechanical properties of the welded joint, taking into account the effect of features of all regions, such as microstructure, chemical composition and the area fraction, on the macroscopic mechanical properties of the welded joint. Hot rolled DP 600 steel was gas metal arc welded (GMAW) and tensile specimens were made with a welded joint. In the heat-affected zone (HAZ), the microstructure varied from bainite to coarse grained ferrite and tempered martensite. Chemical composition of every quantified region in the welded specimen was also identified using electron probe microanalysis (EPMA). Macromechanical FE modelling was employed to simulate the mechanical properties of the welded tensile specimen. 2D representative volume elements (RVE) for different parts of the welded region were constructed from real microstructure. 2D simulated flow curves were corrected to 3Ds using a developed correlation factor. Finally, the tensile test of welded material with inhomogeneous morphology was simulated and good agreement between experimental and predicted flow curve was achieved.

  19. Alterations of in vivo CA1 network activity in Dp(16)1Yey Down syndrome model mice.

    Science.gov (United States)

    Raveau, Matthieu; Polygalov, Denis; Boehringer, Roman; Amano, Kenji; Yamakawa, Kazuhiro; McHugh, Thomas J

    2018-02-27

    Down syndrome, the leading genetic cause of intellectual disability, results from an extra-copy of chromosome 21. Mice engineered to model this aneuploidy exhibit Down syndrome-like memory deficits in spatial and contextual tasks. While abnormal neuronal function has been identified in these models, most studies have relied on in vitro measures. Here, using in vivo recording in the Dp(16)1Yey model, we find alterations in the organization of spiking of hippocampal CA1 pyramidal neurons, including deficits in the generation of complex spikes. These changes lead to poorer spatial coding during exploration and less coordinated activity during sharp-wave ripples, events involved in memory consolidation. Further, the density of CA1 inhibitory neurons expressing neuropeptide Y, a population key for the generation of pyramidal cell bursts, were significantly increased in Dp(16)1Yey mice. Our data refine the 'over-suppression' theory of Down syndrome pathophysiology and suggest specific neuronal subtypes involved in hippocampal dysfunction in these model mice. © 2018, Raveau et al.

  20. Use of computer tomography and 3DP Rapid Prototyping technique in cranioplasty planning - analysis of accuracy of bone defect modeling

    International Nuclear Information System (INIS)

    Markowska, O.; Gardzinska, A.; Miechowicz, S.; Chrzan, R.; Urbanik, A.; Miechowicz, S.

    2009-01-01

    Background: The accuracy considerations of alignment of skull bone loss and artificial model of implant are presented. In standard surgical treatment the application of prefabricated alloplastic implants requires complicated procedures during surgery, especially additional geometry processing to provide better adjustment of implant. Rapid Prototyping can be used as an effective tool to generate complex 3D medical models and to improve and simplify surgical treatment planning. The operation time can also be significantly reduced. The aim of the study is adjustment accuracy analysis by measurements of fissure between the bone loss and implant. Material/Methods: The 3D numerical model was obtained from CT imaging with Siemens Sensation 10 CT scanner. The physical models were fabricated with 3DP Rapid Prototyping technology. The measurements were performed in determined points of the bone loss and implant borders. Results: Maximal width of fissure between bone loss and implant was 1.8 mm and minimal 0 mm. Average width was 0.714 mm, standard deviation 0.663 mm. Conclusions: Accuracy of 3DP technique is enough to create medical models in selected field of medicine. Models created using RP methods may be then used to produce implants of biocompatible material, for example by vacuum casting. Using of method suggested may allow shortening of presurgery and surgery time. (authors)

  1. Optimization of welding current waveform for dissimilar material with DP590 and Al5052 by Delta-spot welding process

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ji Sun; Kim, In Ju; Kim, Young Gon [Korea Institute of Industrial Technology, Gwangju (Korea, Republic of)

    2016-06-15

    The automotive industry has a target goal to improve fuel consumption due to restricted exhaust gas regulation. For this reason, the applicability of lightweight material, Al alloys, Mg alloys is also being expanded. In this concept, high strength steel, DP590 and light alloy, AL5052 are joined in the right place of the car body. However, it is difficult to join to steel and aluminum by conventional fusion welding. Generally, in respect to dissimilar metal joining by fusion welding, intermetallic compound layer is formed at the joint interface, hot cracking is generated. In this study, the effect of the current waveform on the mechanical characteristics and microstructure in Delta spot welding process of dissimilar metal was investigated. As results, Intermetallic compound (IMC) layer was reduced from 2.355 μm to 1.09 μm by using Delta spot welding process; also the welding current range improved by 50% in the delta spot welding, higher than in the inverter resistance welding. To conclude, the delta spot welding process adopting the process tapes contributes to improving the welding quality for dissimilar metals (Al5052 and DP590) due to a decrease in IMC layer.

  2. Micro–macro-characterisation and modelling of mechanical properties of gas metal arc welded (GMAW) DP600 steel

    International Nuclear Information System (INIS)

    Ramazani, A.; Mukherjee, K.; Abdurakhmanov, A.; Prahl, U.; Schleser, M.; Reisgen, U.; Bleck, W.

    2014-01-01

    Dual-phase (DP) steels show combined high strength and adequate formability. However, during welding, their microstructural feature of dispersion of hard martensite islands in the soft ferrite matrix is lost and the properties deteriorate. The current research aims to study the mechanical properties of the welded joint, taking into account the effect of features of all regions, such as microstructure, chemical composition and the area fraction, on the macroscopic mechanical properties of the welded joint. Hot rolled DP 600 steel was gas metal arc welded (GMAW) and tensile specimens were made with a welded joint. In the heat-affected zone (HAZ), the microstructure varied from bainite to coarse grained ferrite and tempered martensite. Chemical composition of every quantified region in the welded specimen was also identified using electron probe microanalysis (EPMA). Macromechanical FE modelling was employed to simulate the mechanical properties of the welded tensile specimen. 2D representative volume elements (RVE) for different parts of the welded region were constructed from real microstructure. 2D simulated flow curves were corrected to 3Ds using a developed correlation factor. Finally, the tensile test of welded material with inhomogeneous morphology was simulated and good agreement between experimental and predicted flow curve was achieved

  3. An experimental study on fracture toughness of resistance spot welded galvanized and ungalvanized DP 450 steel sheets used in automotive body

    Directory of Open Access Journals (Sweden)

    Sevim, Ibrahim

    2016-09-01

    Full Text Available The purpose of this study is to determine fracture toughness of Resistance Spot Welded (RSW Dual Phase (DP steels. RSW of galvanized and ungalvanized DP 450 steel sheets was carried out on spot welding machine. Fracture toughness of RSW joints of galvanized and ungalvanized DP 450 steel sheets was calculated from tensile-shear tests. New empirical equations were developed using Least Squares Method (LSM between energy release rate, fracture toughness and critical crack size depending on the relationship between hardness and fracture toughness values. Results indicated that fracture toughness of joints welded by using RSW increased exponentially while the hardness decreased. In addition, fracture toughness and energy release rate of RSW galvanized DP 450 steel sheets were lower compared to RSW ungalvanized DP 450 steel sheets which had approximately the same hardness.El objetivo de este estudio es determinar la tenacidad de fractura de los aceros dual (DP soldados por puntos de resistencia (RSW. En la máquina de soldadura por puntos se realizó la soldadura de láminas de acero DP 450 galvanizado y sin galvanizar. A partir de los ensayos de tracción-cizallamiento, se calculó la tenacidad a la fractura de las uniones del acero DP 450 galvanizado y sin galvanizar. Aplicando el método de mínimos cuadrados (LSM se desarrollaron nuevas ecuaciones empíricas entre el porcentaje de energía liberada, la tenacidad de fractura y el tamaño de grieta crítica en función de la relación entre los valores de tenacidad de fractura y de dureza. Los resultados indicaron que la tenacidad de fractura de las uniones soldadas por RSW aumentó exponencialmente, mientras que la dureza disminuyó. Además, el porcentaje de energía liberada de las láminas de acero DP 450 galvanizadas y soldadas fueron menores que en el caso de las láminas sin galvanizar a valores iguales de dureza.

  4. Overlapping trisomies for human chromosome 21 orthologs produce similar effects on skull and brain morphology of Dp(16)1Yey and Ts65Dn mice.

    Science.gov (United States)

    Starbuck, John M; Dutka, Tara; Ratliff, Tabetha S; Reeves, Roger H; Richtsmeier, Joan T

    2014-08-01

    Trisomy 21 results in gene-dosage imbalance during embryogenesis and throughout life, ultimately causing multiple anomalies that contribute to the clinical manifestations of Down syndrome. Down syndrome is associated with manifestations of variable severity (e.g., heart anomalies, reduced growth, dental anomalies, shortened life-span). Craniofacial dysmorphology and cognitive dysfunction are consistently observed in all people with Down syndrome. Mouse models are useful for studying the effects of gene-dosage imbalance on development. We investigated quantitative changes in the skull and brain of the Dp(16)1Yey Down syndrome mouse model and compared these mice to Ts65Dn and Ts1Cje mouse models. Three-dimensional micro-computed tomography images of Dp(16)1Yey and euploid mouse crania were morphometrically evaluated. Cerebellar cross-sectional area, Purkinje cell linear density, and granule cell density were evaluated relative to euploid littermates. Skulls of Dp(16)1Yey and Ts65Dn mice displayed similar changes in craniofacial morphology relative to their respective euploid littermates. Trisomy-based differences in brain morphology were also similar in Dp(16)1Yey and Ts65Dn mice. These results validate examination of the genetic basis for craniofacial and brain phenotypes in Dp(16)1Yey mice and suggest that they, like Ts65Dn mice, are valuable tools for modeling the effects of trisomy 21 on development. © 2014 Wiley Periodicals, Inc.

  5. The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo

    Directory of Open Access Journals (Sweden)

    Jehn-Chuan Lee

    2016-08-01

    Full Text Available Oral squamous cell carcinoma (OSCC is a common malignancy with a growing worldwide incidence and prevalence. The N-myc downstream regulated gene (NDRG family of NDRG1, 2, 3, and mammary serine protease inhibitor (Maspin gene are well-known modulators in the neoplasia process. Current research has considered iron chelators as new anti-cancer agents; however, the anticancer activities of iron chelators and their target genes in OSCC have not been well investigated. We showed that iron chelators (Dp44mT, desferrioxamine (DFO, and deferasirox all significantly inhibit SAS cell growth. Flow cytometry further indicated that Dp44mT inhibition of SAS cells growth was partly due to induction of G1 cell cycle arrest. Iron chelators enhanced expressions of NDRG1 and NDRG3 while repressing cyclin D1 expression in OSCC cells. The in vivo antitumor effect on OSCC and safety of Dp44mT were further confirmed through a xenograft animal model. The Dp44mT treatment also increased Maspin protein levels in SAS and OECM-1 cells. NDRG3 knockdown enhanced the growth of OECM-1 cells in vitro and in vivo. Our results indicated that NDRG3 is a tumor suppressor gene in OSCC cells, and Dp44mT could be a promising therapeutic agent for OSCC treatment.

  6. Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils.

    Science.gov (United States)

    Wang, Yanhui; Li, Honghong; Feng, Guojun; Du, Liangwei; Zeng, Dongqiang

    2017-01-01

    A diuron-degrading endophyte DP8-1 was isolated from sugarcane root grown in diuron-treated soil in the present study. The endophyte was identified as Neurospora intermedia based on the morphological characteristics and sequence analysis. The fermentation parameters including temperature, pH, inoculation size, carbon source, and initial diuron concentration were also investigated for the optimization of degradation efficiency. The results indicated that strain DP8-1 was capable of degrading up to 99% diuron within 3 days under the optimal degrading condition. The study of degradation spectrum indicated that strain DP8-1 could also degrade and utilize fenuron, monuron, metobromuron, isoproturon, chlorbromuron, linuron, and chlortoluron as substrate for strain growth. On basis of liquid chromatography-mass spectrometry analysis for the products of the degradation of diuron, strain DP8-1 metabolized diuron to produce N-(3,4-dichlorophenyl)-urea and N-(3,4-dichlorophenyl)-N-methylurea through sequential N-dealkylations. In a soil bioaugmentation experiment, the inoculation of strain DP8-1 into diuron-treated soil effectively enhanced the disappearance rate of diuron.

  7. Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils

    Science.gov (United States)

    Feng, Guojun; Du, Liangwei; Zeng, Dongqiang

    2017-01-01

    A diuron-degrading endophyte DP8-1 was isolated from sugarcane root grown in diuron-treated soil in the present study. The endophyte was identified as Neurospora intermedia based on the morphological characteristics and sequence analysis. The fermentation parameters including temperature, pH, inoculation size, carbon source, and initial diuron concentration were also investigated for the optimization of degradation efficiency. The results indicated that strain DP8-1 was capable of degrading up to 99% diuron within 3 days under the optimal degrading condition. The study of degradation spectrum indicated that strain DP8-1 could also degrade and utilize fenuron, monuron, metobromuron, isoproturon, chlorbromuron, linuron, and chlortoluron as substrate for strain growth. On basis of liquid chromatography-mass spectrometry analysis for the products of the degradation of diuron, strain DP8-1 metabolized diuron to produce N-(3,4-dichlorophenyl)-urea and N-(3,4-dichlorophenyl)-N-methylurea through sequential N-dealkylations. In a soil bioaugmentation experiment, the inoculation of strain DP8-1 into diuron-treated soil effectively enhanced the disappearance rate of diuron. PMID:28809955

  8. KIR And HLA Haplotype Analysis in a Family Lacking The KIR 2DL1-2DP1 Genes

    Directory of Open Access Journals (Sweden)

    Vojvodić Svetlana

    2015-06-01

    Full Text Available The killer cell immunoglobulin-like receptor (KIR gene cluster exhibits extensive allelic and haplotypic diversity that is observed as presence/absence of genes, resulting in expansion and contraction of KIR haplotypes and by allelic variation of individual KIR genes. We report a case of KIR pseudogene 2DP1 and 2DL1 gene absence in members of one family with the children suffering from acute myelogenous leukemia (AML. Killer cell immunoglo-bulin-like receptor low resolution genotyping was performed by the polymerase chain reaction (PCR-sequencespecific primers (SSP/sequence-specific oligonucleotide (SSO method and haplotype assignment was done by gene content analysis. Both parents and the maternal grandfather, shared the same Cen-B2 KIR haplotype, containing KIR 3DL3, -2DS2, -2DL2 and -3DP1 genes. The second haplotype in the KIR genotype of the mother and grandfather was Tel-A1 with KIR 2DL4 (normal and deleted variant, -3DL1, -22 bp deletion variant of the 2DS4 gene and -3DL2, while the second haplotype in the KIR genotype of the father was Tel-B1 with 2DL4 (normal variant, -3DS1, -2DL5, -2DS5, -2DS1 and 3DL2 genes. Haplotype analysis in all three offsprings revealed that the children inherited the Cen-B2 haplotype with the same gene content but two of the children inherited a deleted variant of the 2DL4 gene, while the third child inherited a normal one. The second haplotype of all three offspring contained KIR 2DL4, -2DL5, -2DS1, -2DS4 (del 22bp variant, -2DS5, -3DL1 and -3DL2 genes, which was the basis of the assumption that there is a hybrid haplotype and that the present 3DL1 gene is a variant of the 3DS1 gene. Due to consanguinity among the ancestors, the results of KIR segregation analysis showed the existence of a very rare KIR genotype in the offspring. The family who is the subject of this case is even more interesting because the father was 10/10 human leukocyte antigen (HLA-matched to his daughter, all members of the family have

  9. On the use of differential solubility in aqueous ethanol solutions to narrow the DP range of food-grade starch hydrolysis products.

    Science.gov (United States)

    Balto, Amy S; Lapis, Trina J; Silver, Rachel K; Ferreira, Andrew J; Beaudry, Christopher M; Lim, Juyun; Penner, Michael H

    2016-04-15

    Considerable research is focused on understanding the functionality of starch hydrolysis products (SHP) consisting of glucose, maltose, maltooligosaccharides (MOS), and maltopolysaccharides (MPS). A confounding factor in this research is the high molecular dispersity of commercially available SHP. The study presented herein characterizes a flexible fractionation approach for lowering the dispersity of such products. This was accomplished by fractionating a corn syrup solids (CSS) preparation based on the differential solubility of its component saccharides in aqueous-ethanol solutions. Products obtained from selected fractionations were characterized with respect to degree of polymerization (DP; liquid chromatography), dextrose equivalency (reducing sugar assays), and prevalence of branching (NMR). Glucose and maltose were preferentially removed from CSS using high (⩾90%) ethanol extractants. Preparations with relatively narrow ranges of MOS, lower DP MPS, and higher DP MPS were obtained through repetitive 70%-ethanol extractions. Linear, as opposed to branched, MOS and MPS were preferentially extracted under all conditions tested. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. White Sands Missile Range 2007 Urban Study: Data Processing - Volume DP-1 (Sonic Calibration)

    Science.gov (United States)

    2008-09-01

    calibrated in a wind tunnel against a NIST standard were positioned at the two ends of the lineup , closest to the buildings (positions 1 and 10 in...utilized westerly winds between 8 and 10 m/s (2007 February 24, 0600–0605 LT). The 5 min of data utilized by the “fast” case were sequential . The...2.4–11.2 m/s. The 5-min “variable” case utilized sequential minutes. Results from all three WS conditions were the following (Vaucher, 2007a

  11. The position of a standard optical computer mouse affects cardiorespiratory responses during the operation of a computer under time constraints.

    Science.gov (United States)

    Sako, Shunji; Sugiura, Hiromichi; Tanoue, Hironori; Kojima, Makoto; Kono, Mitsunobu; Inaba, Ryoichi

    2014-08-01

    This study investigated the association between task-induced stress and fatigue by examining the cardiovascular responses of subjects using different mouse positions while operating a computer under time constraints. The study was participated by 16 young, healthy men and examined the use of optical mouse devices affixed to laptop computers. Two mouse positions were investigated: (1) the distal position (DP), in which the subjects place their forearms on the desk accompanied by the abduction and flexion of their shoulder joints, and (2) the proximal position (PP), in which the subjects place only their wrists on the desk without using an armrest. The subjects continued each task for 16 min. We assessed differences in several characteristics according to mouse position, including expired gas values, autonomic nerve activities (based on cardiorespiratory responses), operating efficiencies (based on word counts), and fatigue levels (based on the visual analog scale - VAS). Oxygen consumption (VO(2)), the ratio of inspiration time to respiration time (T(i)/T(total)), respiratory rate (RR), minute ventilation (VE), and the ratio of expiration to inspiration (Te/T(i)) were significantly lower when the participants were performing the task in the DP than those obtained in the PP. Tidal volume (VT), carbon dioxide output rates (VCO(2)/VE), and oxygen extraction fractions (VO(2)/VE) were significantly higher for the DP than they were for the PP. No significant difference in VAS was observed between the positions; however, as the task progressed, autonomic nerve activities were lower and operating efficiencies were significantly higher for the DP than they were for the PP. Our results suggest that the DP has fewer effects on cardiorespiratory functions, causes lower levels of sympathetic nerve activity and mental stress, and produces a higher total workload than the PP. This suggests that the DP is preferable to the PP when operating a computer.

  12. The position of a standard optical computer mouse affects cardiorespiratory responses during the operation of a computer under time constraints

    Directory of Open Access Journals (Sweden)

    Shunji Sako

    2014-08-01

    Full Text Available Objectives: This study investigated the association between task-induced stress and fatigue by examining the cardiovascular responses of subjects using different mouse positions while operating a computer under time constraints. Material and Methods: The study was participated by 16 young, healthy men and examined the use of optical mouse devices affixed to laptop computers. Two mouse positions were investigated: (1 the distal position (DP, in which the subjects place their forearms on the desk accompanied by the abduction and flexion of their shoulder joints, and (2 the proximal position (PP, in which the subjects place only their wrists on the desk without using an armrest. The subjects continued each task for 16 min. We assessed differences in several characteristics according to mouse position, including expired gas values, autonomic nerve activities (based on cardiorespiratory responses, operating efficiencies (based on word counts, and fatigue levels (based on the visual analog scale – VAS. Results: Oxygen consumption (VO2, the ratio of inspiration time to respiration time (Ti/Ttotal, respiratory rate (RR, minute ventilation (VE, and the ratio of expiration to inspiration (Te/Ti were significantly lower when the participants were performing the task in the DP than those obtained in the PP. Tidal volume (VT, carbon dioxide output rates (VCO2/VE, and oxygen extraction fractions (VO2/VE were significantly higher for the DP than they were for the PP. No significant difference in VAS was observed between the positions; however, as the task progressed, autonomic nerve activities were lower and operating efficiencies were significantly higher for the DP than they were for the PP. Conclusions: Our results suggest that the DP has fewer effects on cardiorespiratory functions, causes lower levels of sympathetic nerve activity and mental stress, and produces a higher total workload than the PP. This suggests that the DP is preferable to the PP when

  13. Arsenic-induced dose-dependent modulation of the NF-κB/IL-6 axis in thymocytes triggers differential immune responses

    International Nuclear Information System (INIS)

    Choudhury, Sreetama; Gupta, Payal; Ghosh, Sayan; Mukherjee, Sudeshna; Chakraborty, Priyanka; Chatterji, Urmi; Chattopadhyay, Sreya

    2016-01-01

    Highlights: • We for the first time explicitly show that arsenic exposure causes morphological damage to the thymus and results in heightened death of thymocytes. • Our data suggests that arsenic-induced apoptosis occurs due to increase in cellular oxidative and nitrosative stress. • We have for the first time established a non-classical role of NF-κB, correlating it with increase in FoxP3 expression. • The % of CD4+ CD25+ T cells were high and expression of FoxP3 has also increased at higher doses of arsenic indicating an nTreg bias. - Abstract: Arsenic contamination of drinking water is a matter of global concern. Arsenic intake impairs immune responses and leads to a variety of pathological conditions including cancer. In order to understand the intricate tuning of immune responses elicited by chronic exposure to arsenic, a mouse model was established by subjecting mice to different environmentally relevant concentrations of arsenic in drinking water for 30 days. Detailed study of the thymus, a primary immune organ, revealed arsenic-mediated tissue damage in both histological specimens and scanning electron micrographs. Analysis of molecular markers of apoptosis by Western blot revealed a dose-dependent activation of the apoptotic cascade. Enzymatic assays supported oxidative stress as an instigator of cell death. Interestingly, assessment of inflammatory responses revealed disparity in the NF-κB/IL-6/STAT3 axis, where it was found that in animals consuming higher amounts of arsenic NF-κB activation did not lead to the classical IL-6 upregulation response. This deviation from the canonical pathway was accompanied with a significant rise in numbers of CD4+ CD25+ FoxP3 expressing cells in the thymus. The cytokine profile of the animals exposed to higher doses of arsenic also indicated an immune-suppressed milieu, thus validating that arsenic shapes the immune environment in context to its dose of exposure and that at higher doses it leads to immune

  14. Induction of p53-mediated apoptosis in splenocytes and thymocytes of C57BL/6 mice exposed to perfluorooctane sulfonate (PFOS)

    International Nuclear Information System (INIS)

    Dong, Guang-Hui; Wang, Jing; Zhang, Ying-Hua; Liu, Miao-Miao; Wang, Da; Zheng, Li; Jin, Yi-He

    2012-01-01

    Perfluorooctane sulfonate (PFOS) is a persistent environmental contaminant found in human and wildlife tissues. It has been reported that PFOS can cause atrophy of the immune organs and apoptosis of immunocytes in rodents. However, the mechanism behind such cause is still unclear. To understand the model of cell death and its mechanism on lymphoid cells in vivo, we conducted a dose/response experiment in which 4 groups of male adult C57BL/6 mice (12 mice per group) were dosed daily by oral gavage with PFOS at 0, 0.0167, 0.0833, or 0.8333 mg/kg/day, yielding targeted Total Administered Dose (TAD) of 0, 1, 5, or 50 mg PFOS/kg, respectively, over 60 days. The results showed that spleen and thymus weight were significantly reduced in the highest PFOS-dose-group (TAD 50 mg PFOS/kg) compared to the control group, whereas liver weight was significantly increased. We analyzed the cell death via apoptosis with an annexin-V/propidium iodide assay by flow cytometry, and observed that both the percentage of apoptosis and the expression of the pro-apoptotic proteins p53 in splenocytes and thymocytes increased in a dose-related manner after PFOS treatment. We also observed that PFOS induced p53-dependent apoptosis through the cooperation between the Bcl-xl down regulation without changing the Bcl-2 and Bax expression. The down regulation of Bcl-xl was strongly indicating mitochondrial involvement in apoptosis. It is confirmed by the release of cytochrome c and activation of caspase-3. All of these findings establish an important role of p53 and mitochondrial function in PFOS induced toxic environment in the host. -- Highlights: ► PFOS immunotoxicity is caused by induction of apoptosis via the p53 activation. ► PFOS exposure can induce down regulation of Bcl-xl. ► Mitochondria are involved in PFOS-induced apoptosis. ► PFOS exposure can cause the release of cytochrome c and activation of caspase-3.

  15. Induction of p53-mediated apoptosis in splenocytes and thymocytes of C57BL/6 mice exposed to perfluorooctane sulfonate (PFOS)

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Guang-Hui, E-mail: ghdong@mail.cmu.edu.cn [School of Public Health, China Medical University, Shenyang 110001 (China); Wang, Jing [Department of Biostatistics, School of Public Health, Saint Louis University, Saint Louis, MO 63104 (United States); Zhang, Ying-Hua; Liu, Miao-Miao; Wang, Da [School of Public Health, China Medical University, Shenyang 110001 (China); Zheng, Li [Department of Immunology, College of Basic Medical Science, China Medical University, Shenyang 110001 (China); Jin, Yi-He [School of Public Health, China Medical University, Shenyang 110001 (China); School of Environmental Science and Technology, Dalian University of Technology, Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, Dalian 116024 (China)

    2012-10-15

    Perfluorooctane sulfonate (PFOS) is a persistent environmental contaminant found in human and wildlife tissues. It has been reported that PFOS can cause atrophy of the immune organs and apoptosis of immunocytes in rodents. However, the mechanism behind such cause is still unclear. To understand the model of cell death and its mechanism on lymphoid cells in vivo, we conducted a dose/response experiment in which 4 groups of male adult C57BL/6 mice (12 mice per group) were dosed daily by oral gavage with PFOS at 0, 0.0167, 0.0833, or 0.8333 mg/kg/day, yielding targeted Total Administered Dose (TAD) of 0, 1, 5, or 50 mg PFOS/kg, respectively, over 60 days. The results showed that spleen and thymus weight were significantly reduced in the highest PFOS-dose-group (TAD 50 mg PFOS/kg) compared to the control group, whereas liver weight was significantly increased. We analyzed the cell death via apoptosis with an annexin-V/propidium iodide assay by flow cytometry, and observed that both the percentage of apoptosis and the expression of the pro-apoptotic proteins p53 in splenocytes and thymocytes increased in a dose-related manner after PFOS treatment. We also observed that PFOS induced p53-dependent apoptosis through the cooperation between the Bcl-xl down regulation without changing the Bcl-2 and Bax expression. The down regulation of Bcl-xl was strongly indicating mitochondrial involvement in apoptosis. It is confirmed by the release of cytochrome c and activation of caspase-3. All of these findings establish an important role of p53 and mitochondrial function in PFOS induced toxic environment in the host. -- Highlights: ► PFOS immunotoxicity is caused by induction of apoptosis via the p53 activation. ► PFOS exposure can induce down regulation of Bcl-xl. ► Mitochondria are involved in PFOS-induced apoptosis. ► PFOS exposure can cause the release of cytochrome c and activation of caspase-3.

  16. dp53 Restrains ectopic neural stem cell formation in the Drosophila brain in a non-apoptotic mechanism involving Archipelago and cyclin E.

    Directory of Open Access Journals (Sweden)

    Yingshi Ouyang

    Full Text Available Accumulating evidence suggests that tumor-initiating stem cells or cancer stem cells (CSCs possibly originating from normal stem cells may be the root cause of certain malignancies. How stem cell homeostasis is impaired in tumor tissues is not well understood, although certain tumor suppressors have been implicated. In this study, we use the Drosophila neural stem cells (NSCs called neuroblasts as a model to study this process. Loss-of-function of Numb, a key cell fate determinant with well-conserved mammalian counterparts, leads to the formation of ectopic neuroblasts and a tumor phenotype in the larval brain. Overexpression of the Drosophila tumor suppressor p53 (dp53 was able to suppress ectopic neuroblast formation caused by numb loss-of-function. This occurred in a non-apoptotic manner and was independent of Dacapo, the fly counterpart of the well-characterized mammalian p53 target p21 involved in cellular senescence. The observation that dp53 affected Edu incorporation into neuroblasts led us to test the hypothesis that dp53 acts through regulation of factors involved in cell cycle progression. Our results show that the inhibitory effect of dp53 on ectopic neuroblast formation was mediated largely through its regulation of Cyclin E (Cyc E. Overexpression of Cyc E was able to abrogate dp53's ability to rescue numb loss-of-function phenotypes. Increasing Cyc E levels by attenuating Archipelago (Ago, a recently identified transcriptional target of dp53 and a negative regulator of Cyc E, had similar effects. Conversely, reducing Cyc E activity by overexpressing Ago blocked ectopic neuroblast formation in numb mutant. Our results reveal an intimate connection between cell cycle progression and NSC self-renewal vs. differentiation control, and indicate that p53-mediated regulation of ectopic NSC self-renewal through the Ago/Cyc E axis becomes particularly important when NSC homeostasis is perturbed as in numb loss-of-function condition. This has

  17. Mapping microscopic order in plant and mammalian cells and tissues: novel differential polarization attachment for new generation confocal microscopes (DP-LSM)

    Science.gov (United States)

    Steinbach, G.; Pawlak, K.; Pomozi, I.; Tóth, E. A.; Molnár, A.; Matkó, J.; Garab, G.

    2014-03-01

    Elucidation of the molecular architecture of complex, highly organized molecular macro-assemblies is an important, basic task for biology. Differential polarization (DP) measurements, such as linear (LD) and circular dichroism (CD) or the anisotropy of the fluorescence emission (r), which can be carried out in a dichrograph or spectrofluorimeter, respectively, carry unique, spatially averaged information about the molecular organization of the sample. For inhomogeneous samples—e.g. cells and tissues—measurements on macroscopic scale are not satisfactory, and in some cases not feasible, thus microscopic techniques must be applied. The microscopic DP-imaging technique, when based on confocal laser scanning microscope (LSM), allows the pixel by pixel mapping of anisotropy of a sample in 2D and 3D. The first DP-LSM configuration, which, in fluorescence mode, allowed confocal imaging of different DP quantities in real-time, without interfering with the ‘conventional’ imaging, was built on a Zeiss LSM410. It was demonstrated to be capable of determining non-confocally the linear birefringence (LB) or LD of a sample and, confocally, its FDLD (fluorescence detected LD), the degree of polarization (P) and the anisotropy of the fluorescence emission (r), following polarized and non-polarized excitation, respectively (Steinbach et al 2009 Acta Histochem.111 316-25). This DP-LSM configuration, however, cannot simply be adopted to new generation microscopes with considerably more compact structures. As shown here, for an Olympus FV500, we designed an easy-to-install DP attachment to determine LB, LD, FDLD and r, in new-generation confocal microscopes, which, in principle, can be complemented with a P-imaging unit, but specifically to the brand and type of LSM.

  18. Mapping microscopic order in plant and mammalian cells and tissues: novel differential polarization attachment for new generation confocal microscopes (DP-LSM)

    International Nuclear Information System (INIS)

    Steinbach, G; Pawlak, K; Garab, G; Pomozi, I; Tóth, E A; Molnár, A; Matkó, J

    2014-01-01

    Elucidation of the molecular architecture of complex, highly organized molecular macro-assemblies is an important, basic task for biology. Differential polarization (DP) measurements, such as linear (LD) and circular dichroism (CD) or the anisotropy of the fluorescence emission (r), which can be carried out in a dichrograph or spectrofluorimeter, respectively, carry unique, spatially averaged information about the molecular organization of the sample. For inhomogeneous samples—e.g. cells and tissues—measurements on macroscopic scale are not satisfactory, and in some cases not feasible, thus microscopic techniques must be applied. The microscopic DP-imaging technique, when based on confocal laser scanning microscope (LSM), allows the pixel by pixel mapping of anisotropy of a sample in 2D and 3D. The first DP-LSM configuration, which, in fluorescence mode, allowed confocal imaging of different DP quantities in real-time, without interfering with the ‘conventional’ imaging, was built on a Zeiss LSM410. It was demonstrated to be capable of determining non-confocally the linear birefringence (LB) or LD of a sample and, confocally, its FDLD (fluorescence detected LD), the degree of polarization (P) and the anisotropy of the fluorescence emission (r), following polarized and non-polarized excitation, respectively (Steinbach et al 2009 Acta Histochem.111 316–25). This DP-LSM configuration, however, cannot simply be adopted to new generation microscopes with considerably more compact structures. As shown here, for an Olympus FV500, we designed an easy-to-install DP attachment to determine LB, LD, FDLD and r, in new-generation confocal microscopes, which, in principle, can be complemented with a P-imaging unit, but specifically to the brand and type of LSM. (paper)

  19. Proposal on the measurements of d-p elastic scattering analyzing powers at 0.3-2.0 GeV at internal target station of the Nuclotron

    International Nuclear Information System (INIS)

    Uesaka, T.; Ladygin, V.P.; Azhgirej, L.S.

    2005-01-01

    A new high-energy beam polarimeter is proposed for the Nuclotron using Internal Target Station. This polarimeter based on the measurement of the asymmetry for the d-p elastic scattering will allow one to measure both vector and tensor components of the deuteron beam polarization simultaneously. For that purpose the measurement of analyzing powers for the d-p elastic scattering at energies T d = 0.88-2 GeV is proposed. The precise measurements of the deuteron analyzing powers over energy range T d 300-2000 MeV can give an irreplaceable clue on the study of spin-dependence of three-nucleon forces

  20. Thermal effects on the enhanced ductility in non-monotonic uniaxial tension of DP780 steel sheet

    Science.gov (United States)

    Majidi, Omid; Barlat, Frederic; Korkolis, Yannis P.; Fu, Jiawei; Lee, Myoung-Gyu

    2016-11-01

    To understand the material behavior during non-monotonic loading, uniaxial tension tests were conducted in three modes, namely, the monotonic loading, loading with periodic relaxation and periodic loading-unloadingreloading, at different strain rates (0.001/s to 0.01/s). In this study, the temperature gradient developing during each test and its contribution to increasing the apparent ductility of DP780 steel sheets were considered. In order to assess the influence of temperature, isothermal uniaxial tension tests were also performed at three temperatures (298 K, 313 K and 328 K (25 °C, 40 °C and 55 °C)). A digital image correlation system coupled with an infrared thermography was used in the experiments. The results show that the non-monotonic loading modes increased the apparent ductility of the specimens. It was observed that compared with the monotonic loading, the temperature gradient became more uniform when a non-monotonic loading was applied.

  1. Studies of Neutron-Rich Nuclei with (d,p) Reactions in Inverse Kinematics at the HRIBF

    International Nuclear Information System (INIS)

    Grzywacz-Jones, Kate L.; Baktash, Cyrus; Bardayan, Daniel W.; Blackmon, Jeff C.; Catford, Wilton N.; Cizewski, Jolie; Fitzgerald, Ryan; Greife, Uwe; Gross, Carl J.; Johnson, Micah; Kozub, Raymond L.; Liang, J. Felix; Livesay, Jake; Ma, Zhanwen; Moazen, Brian H.; Nesaraja, Caroline D.; Shapira, Dan; Smith, Michael Scott; Thomas, Jeffrey S.; Visser, Dale William

    2005-01-01

    Two N=51 isotones have been measured using (d,p) reactions in inverse kinematics at the Holifield Radioactive Beam Facility (HRIBF) of Oak Ridge National Laboratory. Additionally, we have performed a test measurement using a stable 124Sn beam in preparation for measurements of the 2H(130,132Sn,p)131,133Sn reactions. Preliminary results for 83Ge and 85Se suggest a 5/2+ ground state and a 1/2+ first excited state for both isotopes, in agreement with systematics for the N=51 isotones. The excitation energy of the first excited state is shown to drop as the proton number is reduced. Proton angular distributions following the 2H(124Sn,p)125Sn reaction show sensitivity to the l-value of the transfered nucleon and spectroscopic factors are in agreement with previous measurements in normal kinematics.

  2. A DP based scheme for real-time reconfiguration of solar cell arrays exposed to dynamic changing inhomogeneous illuminations

    DEFF Research Database (Denmark)

    Shi, Liping; Brehm, Robert

    2016-01-01

    The overall energy conversion efficiency of solar cell arrays is highly effected by partial shading effects. Especially for solar panel arrays installed in environments which are exposed to inhomogeneous dynamic changing illuminations such as on roof tops of electrical vehicles the overall system...... efficiency is drastically reduced. Dynamic real-time reconfiguration of the solar panel array can reduce effects on the output efficiency due to partial shading. This results in a maximized power output of the panel array when exposed to dynamic changing illuminations. The optimal array configuration...... with respect to shading patterns can be stated as a combinatorial optimization problem and this paper proposes a dynamic programming (DP) based algorithm which finds the optimal feasible solution to reconfigure the solar panel array for maximum efficiency in real-time with linear time complexity. It is shown...

  3. Typing for HLA-D/DR associated DP-antigens with the primed lymphocyte typing (PLT) technique

    DEFF Research Database (Denmark)

    Morling, N; Jakobsen, B K; Platz, P

    1980-01-01

    A total of 74 healthy unrelated random individuals and 36 patients with juvenile rheumatoid arthritis (JRA) were typed for HLA-D antigens with the homozygous typing cell technique and typed for HLA-D/DR associated DP-antigens with the primed lymphocyte typing (PLT) technique. All patients and some...... of the controls were also HLA-DR typed with a limited battery of anti-DR sera. Selected PLT-cells, specific for the HLA-D/DR antigens D/DRw1-8 and the local specificity D"H" were used. The results of the PLT-experiments were evaluated with the Normalized Median Response (NMR) method and the further procedure...

  4. Signal Processing for Wireless Communication MIMO System with Nano- Scaled CSDG MOSFET based DP4T RF Switch.

    Science.gov (United States)

    Srivastava, Viranjay M

    2015-01-01

    In the present technological expansion, the radio frequency integrated circuits in the wireless communication technologies became useful because of the replacement of increasing number of functions, traditional hardware components by modern digital signal processing. The carrier frequencies used for communication systems, now a day, shifted toward the microwave regime. The signal processing for the multiple inputs multiple output wireless communication system using the Metal- Oxide-Semiconductor Field-Effect-Transistor (MOSFET) has been done a lot. In this research the signal processing with help of nano-scaled Cylindrical Surrounding Double Gate (CSDG) MOSFET by means of Double- Pole Four-Throw Radio-Frequency (DP4T RF) switch, in terms of Insertion loss, Isolation, Reverse isolation and Inter modulation have been analyzed. In addition to this a channel model has been presented. Here, we also discussed some patents relevant to the topic.

  5. HLA class II (DR, DQ, DP) in patients with sarcoidosis: evidence of an increased frequency of DRw6

    DEFF Research Database (Denmark)

    Ødum, Niels; Milman, N; Jakobsen, B K

    1991-01-01

    using primed lymphocyte typing. The frequencies of DRw6 were 41.5% in SA patients and 17.9% in controls (relative risk, RR = 3.2, p = 0.00087, p less than 0.05 when corrected). DR3 was decreased to 14.6% in SA patients compared to 25.9% in controls (RR = 0.49, p = 0.07, i.e., not statistically...... significant). The decreased frequency of DR3 was only seen in patients with severe, long-standing disease. In contrast, the DRw6 increase was most pronounced in patients with severe disease (RR = 6.4; p = 0.003). There were no statistically significant deviations in the frequencies of DQ and DP alleles...

  6. Experiment list: SRX099378 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 2.5,9.2,1453 GSM803106: Aio Ikaros knockout chromatin source_name=mouse Ik knockout DP thymocytes || genetic... background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes || chip antibody=hom

  7. Experiment list: SRX099395 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ,37.3,329 GSM803123: H3K27me3 Ikaros knockout chromatin preleukemic stage2 source_name=mouse Ik knockout DP ...thymocytes || genetic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes

  8. Experiment list: SRX099390 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ,38.3,343 GSM803118: H3K27me3 Ikaros knockout chromatin stageI source_name=mouse Ik knockout DP thymocytes |...| genetic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes || developm

  9. Experiment list: SRX099380 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available .7,8.5,497 GSM803108: Mi2beta Ikaros knockout chromatin source_name=mouse Ik knockout DP thymocytes || genet...ic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes || chip antibody=h

  10. Experiment list: SRX099391 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 2.7,812 GSM803119: H3K9ac2 Ikaros knockout chromatin stageI source_name=mouse Ik knockout DP thymocytes || g...enetic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes || development

  11. Experiment list: SRX099394 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 34.8,30309 GSM803122: H3K4me3 Ikaros knockout chromatin preleukemic stage2 source_name=mouse Ik knockout DP ...thymocytes || genetic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes

  12. Experiment list: SRX099396 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 0.5,3585 GSM803124: H3K9ac2 Ikaros knockout chromatin preleukemic stage2 source_name=mouse Ik knockout DP th...ymocytes || genetic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes |

  13. Experiment list: SRX099389 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 26.0,27537 GSM803117: H3K4me3 Ikaros knockout chromatin stageI source_name=mouse Ik knockout DP thymocytes |...| genetic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocytes || developm

  14. Experiment list: SRX099397 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ,11.1,3164 GSM803125: H3K36me3 Ikaros knockout chromatin preleukemic stage2 source_name=mouse Ik knockout DP... thymocytes || genetic background=C57BL/6 x129S4/SvJae || genotype=Ikaros knockout || cell type=DP thymocyte

  15. Identification of residues within the African swine fever virus DP71L protein required for dephosphorylation of translation initiation factor eIF2α and inhibiting activation of pro-apoptotic CHOP

    Energy Technology Data Exchange (ETDEWEB)

    Barber, Claire; Netherton, Chris; Goatley, Lynnette [The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF (United Kingdom); Moon, Alice; Goodbourn, Steve [Institute for Infection and Immunity, St. George' s, University of London, London SW17 0RE (United Kingdom); Dixon, Linda, E-mail: linda.dixon@pirbright.ac.uk [The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF (United Kingdom)

    2017-04-15

    The African swine fever virus DP71L protein recruits protein phosphatase 1 (PP1) to dephosphorylate the translation initiation factor 2α (eIF2α) and avoid shut-off of global protein synthesis and downstream activation of the pro-apoptotic factor CHOP. Residues V16 and F18A were critical for binding of DP71L to PP1. Mutation of this PP1 binding motif or deletion of residues between 52 and 66 reduced the ability of DP71L to cause dephosphorylation of eIF2α and inhibit CHOP induction. The residues LSAVL, between 57 and 61, were also required. PP1 was co-precipitated with wild type DP71L and the mutant lacking residues 52- 66 or the LSAVL motif, but not with the PP1 binding motif mutant. The residues in the LSAVL motif play a critical role in DP71L function but do not interfere with binding to PP1. Instead we propose these residues are important for DP71L binding to eIF2α. - Highlights: •The African swine fever virus DP71L protein recruits protein phosphatase 1 (PP1) to dephosphorylate translation initiation factor eIF2α (eIF2α). •The residues V{sup 16}, F{sup 18} of DP71L are required for binding to the α, β and γ isoforms of PP1 and for DP71L function. •The sequence LSAVL downstream from the PP1 binding site (residues 57–61) are also important for DP71L function. •DP71L mutants of the LSAVL sequence retain ability to co-precipitate with PP1 showing these sequences have a different role to PP1 binding.

  16. The E2F-DP1 Transcription Factor Complex Regulates Centriole Duplication in Caenorhabditis elegans

    Directory of Open Access Journals (Sweden)

    Jacqueline G. Miller

    2016-03-01

    Full Text Available Centrioles play critical roles in the organization of microtubule-based structures, from the mitotic spindle to cilia and flagella. In order to properly execute their various functions, centrioles are subjected to stringent copy number control. Central to this control mechanism is a precise duplication event that takes place during S phase of the cell cycle and involves the assembly of a single daughter centriole in association with each mother centriole . Recent studies have revealed that posttranslational control of the master regulator Plk4/ZYG-1 kinase and its downstream effector SAS-6 is key to ensuring production of a single daughter centriole. In contrast, relatively little is known about how centriole duplication is regulated at a transcriptional level. Here we show that the transcription factor complex EFL-1-DPL-1 both positively and negatively controls centriole duplication in the Caenorhabditis elegans embryo. Specifically, we find that down regulation of EFL-1-DPL-1 can restore centriole duplication in a zyg-1 hypomorphic mutant and that suppression of the zyg-1 mutant phenotype is accompanied by an increase in SAS-6 protein levels. Further, we find evidence that EFL-1-DPL-1 promotes the transcription of zyg-1 and other centriole duplication genes. Our results provide evidence that in a single tissue type, EFL-1-DPL-1 sets the balance between positive and negative regulators of centriole assembly and thus may be part of a homeostatic mechanism that governs centriole assembly.

  17. Hormonal Contraceptives Differentially Suppress TFV and TAF Inhibition of HIV Infection and TFV-DP in Blood and Genital Tract CD4+ T cells.

    Science.gov (United States)

    Shen, Zheng; Rodriguez-Garcia, Marta; Patel, Mickey V; Bodwell, Jack; Kashuba, Angela D M; Wira, Charles R

    2017-12-18

    HIV prevention research is focused on combining antiretrovirals (ARV) and progestin contraceptives to prevent HIV infection and pregnancy. The possibility that progestins compromise ARV anti-HIV activity prompted us to evaluate the effects of progestins on tenofovir (TFV) and TFV-alafenamide (TAF) on HIV infection and intracellular TFV-diphosphate (TFV-DP) concentrations in blood and genital CD4+ T cells. Following incubation of blood CD4+ T cells with TFV or TAF, Medroxyprogesterone acetate (MPA), but not Levonorgestrel, Norethisterone or progesterone, suppressed the anti-HIV effect of TFV by reducing intracellular TFV-DP, but had no effect on TAF inhibition of infection or TFV-DP. In contrast, with genital CD4+ T cells, MPA suppressed TAF inhibition of HIV infection and lowered of TFV-DP concentrations without affecting TFV protection. These findings demonstrate that MPA selectively compromises TFV and TAF protection in blood and genital CD4+ T cells and suggests that MPA may decrease ARV protection in individuals who use ARV intermittently for prevention.

  18. Grammatical and pragmatic properties of the DP in children with Specific Language Impairment (SLI) and in children with High Functioning Autism (HFA)

    NARCIS (Netherlands)

    Creemers, A.; Schaeffer, J.C.; Köhnlein, B.; Audring, J.

    2015-01-01

    This study investigates whether grammar and pragmatics are separate linguistic components or not, and whether children with SLI and children with HFA have overlapping or distinct linguistic profiles. We examine two DP-related phenomena: the mass-count distinction (grammatical) and the choice for a

  19. A BENCHMARKING ANALYSIS FOR FIVE RADIONUCLIDE VADOSE ZONE MODELS (CHAIN, MULTIMED_DP, FECTUZ, HYDRUS, AND CHAIN 2D) IN SOIL SCREENING LEVEL CALCULATIONS

    Science.gov (United States)

    Five radionuclide vadose zone models with different degrees of complexity (CHAIN, MULTIMED_DP, FECTUZ, HYDRUS, and CHAIN 2D) were selected for use in soil screening level (SSL) calculations. A benchmarking analysis between the models was conducted for a radionuclide (99Tc) rele...

  20. DP IV/CD26, APN/CD13 and related enzymes as regulators of T cell immunity: implications for experimental encephalomyelitis and multiple sclerosis.

    Science.gov (United States)

    Reinhold, Dirk; Bank, Ute; Täger, Michael; Ansorge, Siegfried; Wrenger, Sabine; Thielitz, Anja; Lendeckel, Uwe; Faust, Jürgen; Neubert, Klaus; Brocke, Stefan

    2008-01-01

    Multiple sclerosis (MS) is the most frequent demyelinating disease of the central nervous system. Peptidases like dipeptidyl peptidase IV (DP IV, CD26) and aminopeptidase N (APN, CD13) play a regulatory role in T cell activation and represent potential targets for the treatment of inflammatory disorders. Synthetic inhibitors of DP IV and/or APN enzymatic activity induce production of the immunosuppressive cytokine TGF-beta1 and subsequently suppress DNA synthesis and Th1 cytokine production of activated human T cells. Compelling evidence has demonstrated that IL-17-producing CD4 cells (Th17) are a major contributor to the pathogenesis of autoimmune inflammation. Here, we report that inhibitors of DP IV-like activity as well as of APN activity inhibit IL-17 production in activated human and mouse T cells. Combining inhibitors of DP IV and APN increases the suppressive effect on T cell specific IL-17 production in vitro compared to a single peptidase inhibitor. In the following, we summarize the evidence for the role of both ectoenzymes in T cell activation in vitro and in vivo and provide a rationale for the use of combined or dual ectopeptidase inhibitors to treat autoimmune diseases like MS.

  1. Experimental Comparison of Gains in Achievable Information Rates from Probabilistic Shaping and Digital Backpropagation for DP-256QAM/1024QAM WDM Systems

    DEFF Research Database (Denmark)

    Porto da Silva, Edson; Yankov, Metodi Plamenov; Da Ros, Francesco

    2016-01-01

    Gains in achievable information rates from probabilistic shaping and digital backpropagation are compared for WDM transmission of 5 × 10 GBd DP-256QAM/1024QAM up to 1700 km of reach. The combination of both techniques its shown to provide gains of up to ∼0.5 bits/QAM symbol...

  2. TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets

    Directory of Open Access Journals (Sweden)

    Trudy Straetemans

    2012-01-01

    Full Text Available Adoptive therapy with TCR gene-engineered T cells provides an attractive and feasible treatment option for cancer patients. Further development of TCR gene therapy requires the implementation of T-cell target epitopes that prevent “on-target” reactivity towards healthy tissues and at the same time direct a clinically effective response towards tumor tissues. Candidate epitopes that meet these criteria are MAGE-C2336-344/HLA-A2 (MC2/A2 and MAGE-A3243-258/HLA-DP4 (MA3/DP4. We molecularly characterized TCRαβ genes of an MC2/A2-specific CD8 and MA3/DP4-specific CD4 T-cell clone derived from melanoma patients who responded clinically to MAGE vaccination. We identified MC2/A2 and MA3/DP4-specific TCR-Vα3/Vβ28 and TCR-Vα38/Vβ2 chains and validated these TCRs in vitro upon gene transfer into primary human T cells. The MC2 and MA3 TCR were surface-expressed and mediated CD8 T-cell functions towards melanoma cell lines and CD4 T-cell functions towards dendritic cells, respectively. We intend to start testing these MAGE-specific TCRs in phase I clinical trial.

  3. Treatment of tooth fracture by medium-energy CO2 laser and DP-bioactive glass paste: the interaction of enamel and DP-bioactive glass paste during irradiation by CO2 laser.

    Science.gov (United States)

    Lin, C P; Tseng, Y C; Lin, F H; Liao, J D; Lan, W H

    2001-03-01

    Acute trauma or trauma associated with occlusal disturbance can produce tooth crack or fracture. Although several methods are proposed to treat the defect, however, the prognosis is generally poor. If the fusion of a tooth fracture by laser is possible, it will offer an alternative to extraction or at least serve as an adjunctive treatment in the reconstruction. We have tried to use a continuous-wave CO2 laser and a newly developed DP-bioactive glass paste (DPGP) to fuse or bridge tooth crack or fracture lines. Both the DP-bioactive glass paste and tooth enamel have strong absorption bands at the wavelength of 10.6 microm. Therefore, under CO2 laser, DPGP and enamel should have an effective absorption and melt together. The interface between DPGP and enamel could be regarded as a mixture of DPGP and enamel (DPG-E). The study focused on the phase transformation, microstructure, functional group and thermal behavior of DPG-E with or without CO2 laser irradiation, by the analytical techniques of XRD, FTIR, DTA/TGA, and SEM. The results of XRD showed that the main crystal phase in the DPG-E was dicalcium phosphate dihydrate (CaHPO4.2H2O). It changed into CaHPO4, gamma-Ca2P2O7, beta-Ca2P2O7 and finally alpha-Ca2P2O7 with increasing temperature. In the FTIR analysis, the 720 cm(-1) absorption band ascribed to the P-O-P linkage in pyrophosphate rose up and the intensities of the OH- bands reduced after laser irradiation. In regard to the results of DTA/TGA after irradiation, the weight loss decreased due to the removal of part of absorption water and crystallization water by the CO2 laser. SEM micrographs revealed that the melted masses and the plate-like crystals formed a tight chemical bond between the enamel and DPGP. We expect that DPGP with the help of CO2 laser can be an alternative to the treatment of tooth crack or fracture.

  4. RasGRP1, but not RasGRP3, is required for efficient thymic β-selection and ERK activation downstream of CXCR4.

    Directory of Open Access Journals (Sweden)

    Dominic P Golec

    Full Text Available T cell development is a highly dynamic process that is driven by interactions between developing thymocytes and the thymic microenvironment. Upon entering the thymus, the earliest thymic progenitors, called CD4(-CD8(- 'double negative' (DN thymocytes, pass through a checkpoint termed "β-selection" before maturing into CD4(+CD8(+ 'double positive' (DP thymocytes. β-selection is an important developmental checkpoint during thymopoiesis where developing DN thymocytes that successfully express the pre-T cell receptor (TCR undergo extensive proliferation and differentiation towards the DP stage. Signals transduced through the pre-TCR, chemokine receptor CXCR4 and Notch are thought to drive β-selection. Additionally, it has long been known that ERK is activated during β-selection; however the pathways regulating ERK activation remain unknown. Here, we performed a detailed analysis of the β-selection events in mice lacking RasGRP1, RasGRP3 and RasGRP1 and 3. We report that RasGRP1 KO and RasGRP1/3 DKO deficient thymi show a partial developmental block at the early DN3 stage of development. Furthermore, DN3 thymocytes from RasGRP1 and RasGRP1/3 double knock-out thymi show significantly reduced proliferation, despite expression of the TCRβ chain. As a result of impaired β-selection, the pool of TCRβ(+ DN4 is significantly diminished, resulting in inefficient DN to DP development. Also, we report that RasGRP1 is required for ERK activation downstream of CXCR4 signaling, which we hypothesize represents a potential mechanism of RasGRP1 regulation of β-selection. Our results demonstrate that RasGRP1 is an important regulator of proliferation and differentiation at the β-selection checkpoint and functions downstream of CXCR4 to activate the Ras/MAPK pathway.

  5. Latitudinal exposure to DDTs, HCB, PCBs, PBDEs and DP in giant petrels (Macronectes spp.) across the Southern Ocean

    Energy Technology Data Exchange (ETDEWEB)

    Roscales, Jose L., E-mail: jlroscales@iqog.csic.es [Department of Instrumental Analysis and Environmental Chemistry, Institute of Organic Chemistry, CSIC (IQOG-CSIC), Juan de la Cierva 3, 28006 Madrid (Spain); González-Solís, Jacob; Zango, Laura [Institut de Recerca de la Biodiversitat (IRBio) and Department of Animal Biology, Universitat de Barcelona, Av Diagonal 643, 08028 Barcelona (Spain); Ryan, Peter G. [Percy FitzPatrick Institute, DST-NRF Centre of Excellence, University of Cape Town, Rondebosch 7701 (South Africa); Jiménez, Begoña [Department of Instrumental Analysis and Environmental Chemistry, Institute of Organic Chemistry, CSIC (IQOG-CSIC), Juan de la Cierva 3, 28006 Madrid (Spain)

    2016-07-15

    Studies on Persistent Organic Pollutants (POPs) in Antarctic wildlife are scarce, and usually limited to a single locality. As a result, wildlife exposure to POPs across the Southern Ocean is poorly understood. In this study, we report the differential exposure of the major southern ocean scavengers, the giant petrels, to POPs across a wide latitudinal gradient. Selected POPs (PCBs, HCB, DDTs, PBDEs) and related compounds, such as Dechlorane Plus (DP), were analyzed in plasma of southern giant petrels (Macronectes giganteus) breeding on Livingston (62°S 61°W, Antarctica), Marion (46°S 37°E, sub-Antarctic), and Gough (40°S 10°W, cool temperate) islands. Northern giant petrels (Macronectes halli) from Marion Island were also studied. Stable isotope ratios of C and N (δ{sup 13}C and δ{sup 15}N) were used as dietary tracers of the marine habitat and trophic level, respectively. Breeding locality was a major factor explaining petrel exposure to POPs compared with species and sex. Significant relationships between δ{sup 13}C values and POP burdens, at both inter- and intra-population levels, support latitudinal variations in feeding grounds as a key factor in explaining petrel pollutant burdens. Overall, pollutant levels in giant petrels decreased significantly with latitude, but the relative abundance (%) of the more volatile POPs increased towards Antarctica. DP was found at negligible levels compared with legacy POPs in Antarctic seabirds. Spatial POP patterns found in giant petrels match those predicted by global distribution models, and reinforce the hypothesis of atmospheric long-range transport as the main source of POPs in Antarctica. Our results confirm that wildlife movements out of the polar region markedly increase their exposure to POPs. Therefore, strategies for Antarctic wildlife conservation should consider spatial heterogeneity in exposure to marine pollution. Of particular relevance is the need to clarify the exposure of Antarctic predators to

  6. Latitudinal exposure to DDTs, HCB, PCBs, PBDEs and DP in giant petrels (Macronectes spp.) across the Southern Ocean

    International Nuclear Information System (INIS)

    Roscales, Jose L.; González-Solís, Jacob; Zango, Laura; Ryan, Peter G.; Jiménez, Begoña

    2016-01-01

    Studies on Persistent Organic Pollutants (POPs) in Antarctic wildlife are scarce, and usually limited to a single locality. As a result, wildlife exposure to POPs across the Southern Ocean is poorly understood. In this study, we report the differential exposure of the major southern ocean scavengers, the giant petrels, to POPs across a wide latitudinal gradient. Selected POPs (PCBs, HCB, DDTs, PBDEs) and related compounds, such as Dechlorane Plus (DP), were analyzed in plasma of southern giant petrels (Macronectes giganteus) breeding on Livingston (62°S 61°W, Antarctica), Marion (46°S 37°E, sub-Antarctic), and Gough (40°S 10°W, cool temperate) islands. Northern giant petrels (Macronectes halli) from Marion Island were also studied. Stable isotope ratios of C and N (δ 13 C and δ 15 N) were used as dietary tracers of the marine habitat and trophic level, respectively. Breeding locality was a major factor explaining petrel exposure to POPs compared with species and sex. Significant relationships between δ 13 C values and POP burdens, at both inter- and intra-population levels, support latitudinal variations in feeding grounds as a key factor in explaining petrel pollutant burdens. Overall, pollutant levels in giant petrels decreased significantly with latitude, but the relative abundance (%) of the more volatile POPs increased towards Antarctica. DP was found at negligible levels compared with legacy POPs in Antarctic seabirds. Spatial POP patterns found in giant petrels match those predicted by global distribution models, and reinforce the hypothesis of atmospheric long-range transport as the main source of POPs in Antarctica. Our results confirm that wildlife movements out of the polar region markedly increase their exposure to POPs. Therefore, strategies for Antarctic wildlife conservation should consider spatial heterogeneity in exposure to marine pollution. Of particular relevance is the need to clarify the exposure of Antarctic predators to emerging

  7. Interaction Effects of Social Isolation and Peripheral Work Position on Risk of Disability Pension: A Prospective Study of Swedish Women and Men.

    Science.gov (United States)

    Gustafsson, Klas; Marklund, Staffan; Aronsson, Gunnar; Wikman, Anders; Floderus, Birgitta

    2015-01-01

    The study examines various combinations of levels of social isolation in private life and peripheral work position as predictors of disability pension (DP). A second aim was to test the potential interaction effects (above additivity) of social isolation and peripheral work position on the future risk of DP, and to provide results for men and women by age. The study was based on a sample of 45567 women and men from the Swedish population who had been interviewed between 1992 and 2007. Further information on DP and diagnoses was obtained from the Swedish Social Insurance Agency's database (1993-2011). The studied predictors were related to DP using Cox's proportional hazard regression. The analyses were stratified on sex and age (20-39 years, 40-64 years), with control for selected confounders. Increased risks of DP were found for most combinations of social isolation and peripheral work position in all strata. The hazard ratios (HRs) for joint exposure to high degree of social isolation and a peripheral work position were particularly strong among men aged 20-39 (HR 5.70; CI 95% 3.74-8.69) and women aged 20-39 (HR 4.07; CI 2.99-5.56). An interaction effect from combined exposure was found for women in both age groups as well as a tendency in the same direction among young men. However, after confounder control the effects did not reach significance. Individuals who were socially isolated and in a peripheral work position had an increased risk of future DP. The fact that an interaction effect was found among women indicates that a combination of social isolation and peripheral work position may reinforce adverse health effects. There was no evidence that a peripheral work position can be compensated by a high degree of social intergration in private life.

  8. Phase structures of the black Dp-D(p+4)-brane system in various ensembles II: electrical and thermodynamic stability

    International Nuclear Information System (INIS)

    Xiao, Zhiguang; Zhou, Da

    2015-01-01

    By incorporating the electrical stability condition into the discussion, we continue the study on the thermodynamic phase structures of the Dp-D(p+4) black brane in GG, GC, CG, CC ensembles defined in our previous paper http://dx.doi.org/10.1007/JHEP07(2015)134. We find that including the electrical stability conditions in addition to the thermal stability conditions does not modify the phase structure of the GG ensemble but puts more constraints on the parameter space where black branes can stably exist in GC, CG, CC ensembles. In particular, the van der Waals-like phase structure which was supposed to be present in these ensembles when only thermal stability condition is considered would no longer be visible, since the phase of the small black brane is unstable under electrical fluctuations. However, the symmetry of the phase structure by interchanging the two kinds of brane charges and potentials is still preserved, which is argued to be the result of T-duality.

  9. Influence of shielding gas on the mechanical and metallurgical properties of DP-GMA-welded 5083-H321 aluminum alloy

    Science.gov (United States)

    Koushki, Amin Reza; Goodarzi, Massoud; Paidar, Moslem

    2016-12-01

    In the present research, 6-mm-thick 5083-H321 aluminum alloy was joined by the double-pulsed gas metal arc welding (DP-GMAW) process. The objective was to investigate the influence of the shielding gas composition on the microstructure and properties of GMA welds. A macrostructural study indicated that the addition of nitrogen and oxygen to the argon shielding gas resulted in better weld penetration. Furthermore, the tensile strength and bending strength of the welds were improved when oxygen and nitrogen (at concentrations as high as approximately 0.1vol%) were added to the shielding gas; however, these properties were adversely affected when the oxygen and nitrogen contents were increased further. This behavior was attributed to the formation of excessive brown and black oxide films on the bead surface, the formation of intermetallic compounds in the weld metal, and the formation of thicker oxide layers on the bead surface with increasing nitrogen and oxygen contents in the argon-based shielding gas. Analysis by energy-dispersive X-ray spectroscopy revealed that most of these compounds are nitrides or oxides.

  10. Morphology and distribution of martensite in dual phase (DP980) steel and its relation to the multiscale mechanical behavior

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Fan, E-mail: fan.zhang@wsu.edu [School of Mechanical and Material Eng., Washington State University (United States); Ruimi, Annie [Department of Mechanical Eng., Texas A& M University at Qatar, Doha (Qatar); Wo, Pui Ching; Field, David P. [School of Mechanical and Material Eng., Washington State University (United States)

    2016-04-06

    Among generations of advanced high-strength steel alloys, dual-phase steels exhibit a unique combination of strength and formability making them excellent candidates for use in the automotive industry. In this study, we seek to establish a relation between mechanical properties and microstructure of DP980. Electron backscatter diffraction (EBSD)and nanoindentation are used to identify and characterize martensite and ferrite phases. Spatial distributions of martensite and ferrite phases of subjected to various annealing treatments are found using a 2-point correlation function. Micro- and macro-mechanical properties are measured with nanoindentation, Vickers hardness and tensile tests and the results are used to determine the relation between martensite and ferrite phases and the strength of the metal. During the annealing/recovery process, the strength of the martensite phase decreases, the dislocation structure relaxes in the phase boundary region of the ferrite, and the martensite alignment along the rolling direction decreases resulting in the observed metal strength reduction. It is also shown that the higher the annealing temperature, the more homogeneous and equiaxed the distribution of martensite.

  11. Investigation of 112Cd via the (d,p) Reaction and a Reassessment of the Quadrupole-Octupole Coupled Excitation

    Science.gov (United States)

    Jamieson, D. S.; Garrett, P. E.; Ball, G. C.; Demand, G. A.; Faestermann, T.; Finlay, P.; Green, K. L.; Hertenberger, R.; Leach, K. G.; Phillips, A. A.; Sumithrarachchi, C. S.; Svensson, C. E.; Triambak, S.; Wirth, H.-F.; Wong, J.

    The single-particle neutron states in 112Cd have been probed with the 111Cd(d,p) reaction. Beams of up to 1.2 µA of polarized 22 MeV deuterons bombarded 111Cd targets. The reaction protons were momentum analyzed with a Q3D magnetic spectrograph, with spectra were recorded at 10 angles between 10 and 60° with a resolution of 6-7 keV FWHM. In addition to the (d,p) transfer data, (d,d) elastic-scattering data were also obtained and used to ascertain the proper optical model parameters. Cross sections and analyzing powers for all levels observed to be populated were fit to results of DWBA and ADWA calculations, and spectroscopic factors were determined. The 5- level at 2373 keV, previously assigned as a member on the quadrupole-octupole quintuplet set of states because of its enhanced B(E2;5 - to 31 - ) value, was observed to be one of the strongest peaks in the spectrum, and is reassigned as the s1/2 otimes h11/2 two-quasineutron configuration.

  12. Prediction of fracture initiation in square cup drawing of DP980 using an anisotropic ductile fracture criterion

    Science.gov (United States)

    Park, N.; Huh, H.; Yoon, J. W.

    2017-09-01

    This paper deals with the prediction of fracture initiation in square cup drawing of DP980 steel sheet with the thickness of 1.2 mm. In an attempt to consider the influence of material anisotropy on the fracture initiation, an uncoupled anisotropic ductile fracture criterion is developed based on the Lou—Huh ductile fracture criterion. Tensile tests are carried out at different loading directions of 0°, 45°, and 90° to the rolling direction of the sheet using various specimen geometries including pure shear, dog-bone, and flat grooved specimens so as to calibrate the parameters of the proposed fracture criterion. Equivalent plastic strain distribution on the specimen surface is computed using Digital Image Correlation (DIC) method until surface crack initiates. The proposed fracture criterion is implemented into the commercial finite element code ABAQUS/Explicit by developing the Vectorized User-defined MATerial (VUMAT) subroutine which features the non-associated flow rule. Simulation results of the square cup drawing test clearly show that the proposed fracture criterion is capable of predicting the fracture initiation with sufficient accuracy considering the material anisotropy.

  13. Morphology and distribution of martensite in dual phase (DP980) steel and its relation to the multiscale mechanical behavior

    International Nuclear Information System (INIS)

    Zhang, Fan; Ruimi, Annie; Wo, Pui Ching; Field, David P.

    2016-01-01

    Among generations of advanced high-strength steel alloys, dual-phase steels exhibit a unique combination of strength and formability making them excellent candidates for use in the automotive industry. In this study, we seek to establish a relation between mechanical properties and microstructure of DP980. Electron backscatter diffraction (EBSD)and nanoindentation are used to identify and characterize martensite and ferrite phases. Spatial distributions of martensite and ferrite phases of subjected to various annealing treatments are found using a 2-point correlation function. Micro- and macro-mechanical properties are measured with nanoindentation, Vickers hardness and tensile tests and the results are used to determine the relation between martensite and ferrite phases and the strength of the metal. During the annealing/recovery process, the strength of the martensite phase decreases, the dislocation structure relaxes in the phase boundary region of the ferrite, and the martensite alignment along the rolling direction decreases resulting in the observed metal strength reduction. It is also shown that the higher the annealing temperature, the more homogeneous and equiaxed the distribution of martensite.

  14. Effect of Annealing Temperature on Microstructure and Mechanical Properties of Hot-Dip Galvanizing DP600 Steel

    Science.gov (United States)

    Hai-yan, Sun; Zhi-li, Liu; Yang, Xu; Jian-qiang, Shi; Lian-xuan, Wang

    Hot-dip galvanizing dual phase steel DP600 steel grade with low Si was produced by steel plant and experiments by simulating galvanizing thermal history. The microstructure was observed and analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The effect of different annealing temperatures on the microstructure and mechanical properties of dual-phase steel was also discussed. The experimental results show that the dual-phase steel possesses excellent strength and elongation that match EN10346 600MPa standards. The microstructure is ferrite and martensite. TEM micrograph shows that white ferrite with black martensite islands inlay with a diameter of around 1um and the content of 14 18%. The volume will expand and phase changing take the form of shear transformation when ferrite converted to martensite. So there are high density dislocations in ferrite crystalline grain near martensite. The martensite content growing will be obvious along with annealing temperature going up. But the tendency will be weak when temperature high.

  15. Analysis of the Lankford coefficient evolution at different strain rates for AA6016-T4, DP800 and DC06

    Science.gov (United States)

    Lenzen, Matthias; Merklein, Marion

    2017-10-01

    In the automotive sector, a major challenge is the deep-drawing of modern lightweight sheet metals with limited formability. Thus, conventional material models lack in accuracy due to the complex material behavior. A current field of research takes into account the evolution of the Lankford coefficient. Today, changes in anisotropy under increasing degree of deformation are not considered. Only a consolidated average value of the Lankford coefficient is included in conventional material models. This leads to an increasing error in prediction of the flow behavior and therefore to an inaccurate prognosis of the forming behavior. To increase the accuracy of the prediction quality, the strain dependent Lankford coefficient should be respected, because the R-value has a direct effect on the contour of the associated flow rule. Further, the investigated materials show a more or less extinct rate dependency of the yield stress. For this reason, the rate dependency of the Lankford coefficient during uniaxial tension is focused within this contribution. To quantify the influence of strain rate on the Lankford coefficient, tensile tests are performed for three commonly used materials, the aluminum alloy AA6016-T4, the advanced high strength steel DP800 and the deep drawing steel DC06 at three different strain rates. The strain measurement is carried out by an optical strain measurement system. An evolution of the Lankford coefficient was observed for all investigated materials. Also, an influence of the deformation velocity on the anisotropy could be detected.

  16. Analysis of d/p ratio in Au+Au collisions from the E866 experiment at the AGS

    International Nuclear Information System (INIS)

    Shea, Y.; Garcia-Solis, E.J.; Stanskas, P.J.

    1996-01-01

    High energy nucleus-nucleus collisions are a great interest as a means of creating a new state of matter. The transition of nuclear matter to quark matter is expected to result in a strongly interacting region that lives for a long time and expands to a large volume. In order to understand the properties of the collision region, it is important to gather information experimentally on the lifetime and thermodynamic attributes such as temperature, volume, density, and entropy of the collision region. Deuteron production by phase space coalescence is particularly interesting because it can be used as a probe in studying the space-time structure of the heavy ion collisions. In the hot and dense participant region, a proton and a neutron coalesce when their relative momentum is small. The deuteron density in momentum space is proportional to the proton density squared in momentum space at equal momenta per nucleon, assuming proton and neutron density to be identical. The motivation here is to study the properties of the coalesced deuterons formed in the participant region of Au-Au collisions at 11.6 GeV/c. The d/p ratio as a function of centrality is studied in hopes of gaining information about any change in the size of the participant zone which could lead to the effort of searching for the Quark-Gluon-Plasma at the AGS. The results shown here is very preliminary and the work is in progress

  17. Position Information

    Data.gov (United States)

    Social Security Administration — The Position Information Data Asset provides the ability to search for active SSA position descriptions using various search criteria. An individual may search by PD...

  18. Positive Psychology

    Science.gov (United States)

    Peterson, Christopher

    2009-01-01

    Positive psychology is a deliberate correction to the focus of psychology on problems. Positive psychology does not deny the difficulties that people may experience but does suggest that sole attention to disorder leads to an incomplete view of the human condition. Positive psychologists concern themselves with four major topics: (1) positive…

  19. Ubiquitous positioning

    CERN Document Server

    Mannings, Robin

    2008-01-01

    This groundbreaking resource offers a practical, in-depth understanding of Ubiquitous Positioning - positioning systems that identify the location and position of people, vehicles and objects in time and space in the digitized networked economy. The future and growth of ubiquitous positioning will be fueled by the convergence of many other areas of technology, from mobile telematics, Internet technology, and location systems, to sensing systems, geographic information systems, and the semantic web. This first-of-its-kind volume explores ubiquitous positioning from a convergence perspective, of

  20. Positioning consumption

    DEFF Research Database (Denmark)

    Halkier, Bente; Keller, Margit

    2014-01-01

    positionings emerges based on empirical examples of research in parent–children consumption. Positionings are flexible discursive fixations of the relationship between the performances of the practitioner, other practitioners, media discourse and consumption activities. The basic positioning types...... are the practice maintenance and the practice change position, with different sorts of adapting in between. Media discourse can become a resource for a resistant position against social control or for an appropriating position in favour of space for action. Regardless of the current relation to a particular media......This article analyses the ways in which media discourses become a part of contested consumption activities. We apply a positioning perspective with practice theory to focus on how practitioners relate to media discourse as a symbolic resource in their everyday practices. A typology of performance...

  1. Research on the Microstructures and Mechanical Properties of Ti Micro-Alloyed Cold Rolled Hot-Dip Galvanizing DP980 Steel

    Science.gov (United States)

    Han, Yun; Kuang, Shuang; Qi, Xiumei; Xie, Chunqian; Liu, Guanghui

    Effects of galvanizing simulation parameters on microstructures and mechanical properties of Ti-microalloyed cold rolled hot-dip galvanizing DP980 steel were investigated in this study by optical microscopy (OM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and tensile test. Moreover, the precipitation behavior of Ti in the experimental steel was also studied. The results show that, as the heating temperature increases, the tensile strength of experimental galvanizing DP980 steel decreases while the yield ratio and elongation of the steel are enhanced. The microstructures of experimental steels exhibit typical dual phase steel character and the volume fractions of MA islands are almost 30%. In addition, lots of nano-sized TiC precipitates can be found in the ferrite grains.

  2. DP-THOT - a calculational tool for bundle-specific decay power based on actual irradiation history

    International Nuclear Information System (INIS)

    Johnston, S.; Morrison, C.A.; Albasha, H.; Arguner, D.

    2005-01-01

    A tool has been created for calculating the decay power of an individual fuel bundle to take account of its actual irradiation history, as tracked by the fuel management code SORO. The DP-THOT tool was developed in two phases: first as a standalone executable code for decay power calculation, which could accept as input an entirely arbitrary irradiation history; then as a module integrated with SORO auxiliary codes, which directly accesses SORO history files to retrieve the operating power history of the bundle since it first entered the core. The methodology implemented in the standalone code is based on the ANSI/ANS-5.1-1994 formulation, which has been specifically adapted for calculating decay power in irradiated CANDU reactor fuel, by making use of fuel type specific parameters derived from WIMS lattice cell simulations for both 37 element and 28 element CANDU fuel bundle types. The approach also yields estimates of uncertainty in the calculated decay power quantities, based on the evaluated error in the decay heat correlations built-in for each fissile isotope, in combination with the estimated uncertainty in user-supplied inputs. The method was first implemented in the form of a spreadsheet, and following successful testing against decay powers estimated using the code ORIGEN-S, the algorithm was coded in FORTRAN to create an executable program. The resulting standalone code, DP-THOT, accepts an arbitrary irradiation history and provides the calculated decay power and estimated uncertainty over any user-specified range of cooling times, for either 37 element or 28 element fuel bundles. The overall objective was to produce an integrated tool which could be used to find the decay power associated with any identified fuel bundle or channel in the core, taking into account the actual operating history of the bundles involved. The benefit is that the tool would allow a more realistic calculation of bundle and channel decay powers for outage heat sink planning

  3. 73.7 Tb/s (96x3x256-Gb/s) mode-division-multiplexed DP-16QAM transmission with inline MM-EDFA

    NARCIS (Netherlands)

    Sleiffer, V.A.J.M.; Jung, Y.; Veljanovski, V.; Uden, van R.G.H.; Kuschnerov, M.; Kang, Q.; Grüner-Nielsen, L.; Sun, Y.; Richardson, D.J.; Alam, S.U.; Poletti, F.; Sahu, J.K.; Dhar, A.; Chen, H.; Inan, B.; Koonen, A.M.J.; Corbett, B.; Winfield, R.; Ellis, A.D.; Waardt, de H.

    2012-01-01

    We show transmission of a 73.7 Tb/s (96x3x256-Gb/s) DP-16QAM mode-division- multiplexed signal over 119km of few-mode fiber with inline multi-mode EDFA, using 6x6 MIMO digital signal processing. The total demonstrated net capacity is 57.6 Tb/s (SE 12 bits/s/Hz).

  4. Thymus Atrophy and Double-Positive Escape Are Common Features in Infectious Diseases

    Directory of Open Access Journals (Sweden)

    Juliana de Meis

    2012-01-01

    Full Text Available The thymus is a primary lymphoid organ in which bone marrow-derived T-cell precursors undergo differentiation, leading to migration of positively selected thymocytes to the T-cell-dependent areas of secondary lymphoid organs. This organ can undergo atrophy, caused by several endogenous and exogenous factors such as ageing, hormone fluctuations, and infectious agents. This paper will focus on emerging data on the thymic atrophy caused by infectious agents. We present data on the dynamics of thymus lymphocytes during acute Trypanosoma cruzi infection, showing that the resulting thymus atrophy comprises the abnormal release of thymic-derived T cells and may have an impact on host immune response.

  5. Fevipiprant, an oral prostaglandin DP2 receptor (CRTh2) antagonist, in allergic asthma uncontrolled on low-dose inhaled corticosteroids.

    Science.gov (United States)

    Bateman, Eric D; Guerreros, Alfredo G; Brockhaus, Florian; Holzhauer, Björn; Pethe, Abhijit; Kay, Richard A; Townley, Robert G

    2017-08-01

    Dose-related efficacy and safety of fevipiprant (QAW039), an oral DP 2 (CRTh2) receptor antagonist, was assessed in patients with allergic asthma uncontrolled by low-dose inhaled corticosteroids (ICS).Adult patients were randomised to 12 weeks' treatment with once-daily (1, 3, 10, 30, 50, 75, 150, 300 or 450 mg q.d ) or twice-daily (2, 25, 75 or 150 mg b.i.d ) fevipiprant (n=782), montelukast 10 mg q.d (n=139) or placebo (n=137). All patients received inhaled budesonide 200 μg b.i.d Fevipiprant produced a statistically significant improvement in the primary end-point of change in pre-dose forced expiratory volume in 1 s at week 12 (p=0.0035) with a maximum model-averaged difference to placebo of 0.112 L. The most favourable pairwise comparisons to placebo were for the fevipiprant 150 mg q.d and 75 mg b.i.d groups, with no clinically meaningful differences between q.d and b.i.d Montelukast also demonstrated a significant improvement in this end-point. No impact on other efficacy end-points was observed. Adverse events were generally mild/moderate in severity, and were evenly distributed across doses and treatments.Fevipiprant appears to be efficacious and well-tolerated in this patient population, with an optimum total daily dose of 150 mg. Further investigations into the clinical role of fevipiprant in suitably designed phase III clinical trials are warranted. Copyright ©ERS 2017.

  6. An Experiment of Robust Parallel Algorithm for the Eigenvalue problem of a Multigroup Neutron Diffusion based on modified FETI-DP : Part 2

    International Nuclear Information System (INIS)

    Chang, Jonghwa

    2014-01-01

    Today, we can use a computer cluster consist of a few hundreds CPUs with reasonable budget. Such computer system enables us to do detailed modeling of reactor core. The detailed modeling will improve the safety and the economics of a nuclear reactor by eliminating un-necessary conservatism or missing consideration. To take advantage of such a cluster computer, efficient parallel algorithms must be developed. Mechanical structure analysis community has studied the domain decomposition method to solve the stress-strain equation using the finite element methods. One of the most successful domain decomposition method in terms of robustness is FETI-DP. We have modified the original FETI-DP to solve the eigenvalue problem for the multi-group diffusion problem in previous study. In this study, we report the result of recent modification to handle the three-dimensional subdomain partitioning, and the sub-domain multi-group problem. Modified FETI-DP algorithm has been successfully applied for the eigenvalue problem of multi-group neutron diffusion equation. The overall CPU time is decreasing as number of sub-domains (partitions) is increasing. However, there may be a limit in decrement due to increment of the number of primal points will increase the CPU time spent by the solution of the global equation. Even distribution of computational load (criterion a) is important to achieve fast computation. The subdomain partition can be effectively performed using suitable graph theory partition package such as MeTIS

  7. An Experiment of Robust Parallel Algorithm for the Eigenvalue problem of a Multigroup Neutron Diffusion based on modified FETI-DP : Part 2

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Jonghwa [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-10-15

    Today, we can use a computer cluster consist of a few hundreds CPUs with reasonable budget. Such computer system enables us to do detailed modeling of reactor core. The detailed modeling will improve the safety and the economics of a nuclear reactor by eliminating un-necessary conservatism or missing consideration. To take advantage of such a cluster computer, efficient parallel algorithms must be developed. Mechanical structure analysis community has studied the domain decomposition method to solve the stress-strain equation using the finite element methods. One of the most successful domain decomposition method in terms of robustness is FETI-DP. We have modified the original FETI-DP to solve the eigenvalue problem for the multi-group diffusion problem in previous study. In this study, we report the result of recent modification to handle the three-dimensional subdomain partitioning, and the sub-domain multi-group problem. Modified FETI-DP algorithm has been successfully applied for the eigenvalue problem of multi-group neutron diffusion equation. The overall CPU time is decreasing as number of sub-domains (partitions) is increasing. However, there may be a limit in decrement due to increment of the number of primal points will increase the CPU time spent by the solution of the global equation. Even distribution of computational load (criterion a) is important to achieve fast computation. The subdomain partition can be effectively performed using suitable graph theory partition package such as MeTIS.

  8. Determination of Flavanols and Procyanidins (DP 1-10) in Cocoa-Based Ingredients and Products by UHPLC: First Action 2013.03.

    Science.gov (United States)

    Machonis, Philip R; Jones, Matthew A; Kwik-Uribe, Catherine; Dowell, Dawn

    2014-01-01

    Single-laboratory validation data were reviewed by the Expert Review Panel (ERP) of the Stakeholder Panel on Strategic Food Analytical Methods at the AOAC Mid-Year Meeting, March 12-14, 2013, in Rockville, MD. The ERP determined that the data presented met established standard method performance requirements and adopted a method for determination of flavanols and procyanidins (DP 1-10) in cocoa-based ingredients and products by ultra-HPLC as AOAC Official First Action Method 2013.03 on March 14, 2013. The flavanols and procyanidins (DP 1-10) are eluted using a binary gradient (solvents A and B) consisting of 98 + 2 (v/v) acetonitrile-glacial acetic acid (A) and 95 + 3 + 2 (v/v/v) methanol-water-glacial acetic acid (B). The mobile phase is applied to a diol stationary phase. Detection occurs using fluorescence detection. Recovery of flavanols and procyanidins (DP 1-10) from both high- and low-fat matrixes was 98.4-99.8%. Precision was determined for seven different sample types (cocoa extract, cocoa nib, natural cocoa powder, cocoa liquor, alkalized cocoa powder, dark chocolate, and milk chocolate).

  9. BİNA İÇİ MEKANLARDA PROFIBUS-DP AĞI ÜZERİNDEN DİNAMİK AYDINLATMA DENETİMİ

    OpenAIRE

    Cemal YILMAZ

    2007-01-01

    Bu çalışmada, bina içi mekanlarda Profıbus-DP ağı üzerinden dinamik aydınlatma denetimi gerçekleştirilmiştir. Aydınlatmanın istenilen aydınlık düzeylerine göre ayarlanabilmesi için dimlenebilir armatürler kullanılmıştır. Bina içerisinde aydınlık düzeyini ölçen algılayıcılardan gelen bilgiler Profibus-DP ağı üzerinden merkezi denetim ünitesine aktarılmaktadır. Burada yapılan değerlendirme sonucuna göre aydınlatma armatürlerine kontrol sinyalleri yine Profibus-DP ağı üzerinden gönderilerek dene...

  10. Ceramic-Based 4D Components: Additive Manufacturing (AM) of Ceramic-Based Functionally Graded Materials (FGM) by Thermoplastic 3D Printing (T3DP).

    Science.gov (United States)

    Scheithauer, Uwe; Weingarten, Steven; Johne, Robert; Schwarzer, Eric; Abel, Johannes; Richter, Hans-Jürgen; Moritz, Tassilo; Michaelis, Alexander

    2017-11-28

    In our study, we investigated the additive manufacturing (AM) of ceramic-based functionally graded materials (FGM) by the direct AM technology thermoplastic 3D printing (T3DP). Zirconia components with varying microstructures were additively manufactured by using thermoplastic suspensions with different contents of pore-forming agents (PFA), which were co-sintered defect-free. Different materials were investigated concerning their suitability as PFA for the T3DP process. Diverse zirconia-based suspensions were prepared and used for the AM of single- and multi-material test components. All of the samples were sintered defect-free, and in the end, we could realize a brick wall-like component consisting of dense (<1% porosity) and porous (approx. 5% porosity) zirconia areas to combine different properties in one component. T3DP opens the door to the AM of further ceramic-based 4D components, such as multi-color, multi-material, or especially, multi-functional components.

  11. LKB1 mediates the development of conventional and innate T cells via AMP-dependent kinase autonomous pathways.

    Directory of Open Access Journals (Sweden)

    Marouan Zarrouk

    Full Text Available The present study has examined the role of the serine/threonine kinase LKB1 in the survival and differentiation of CD4/8 double positive thymocytes. LKB1-null DPs can respond to signals from the mature α/β T-cell-antigen receptor and initiate positive selection. However, in the absence of LKB1, thymocytes fail to mature to conventional single positive cells causing severe lymphopenia in the peripheral lymphoid tissues. LKB1 thus appears to be dispensable for positive selection but important for the maturation of positively selected thymocytes. LKB1 also strikingly prevented the development of invariant Vα14 NKT cells and innate TCR αβ gut lymphocytes. Previous studies with gain of function mutants have suggested that the role of LKB1 in T cell development is mediated by its substrate the AMP-activated protein kinase (AMPK. The present study now analyses the impact of AMPK deletion in DP thymocytes and shows that the role of LKB1 during the development of both conventional and innate T cells is mediated by AMPK-independent pathways.

  12. Researcher positioning

    DEFF Research Database (Denmark)

    Mørck, Line Lerche; Khawaja, Iram

    2009-01-01

    abstract  This article focuses on the complex and multi-layered process of researcher positioning, specifically in relation to the politically sensitive study of marginalised and ‘othered' groups such as Muslims living in Denmark. We discuss the impact of different ethnic, religious and racial...... political and personal involvement by the researcher, which challenges traditional perspectives on research and researcher positioning. A key point in this regard is the importance of constant awareness of and reflection on the multiple ways in which one's positioning as a researcher influences the research...

  13. Radiographic positioning

    International Nuclear Information System (INIS)

    Eisenberg, R.L.; Dennis, C.A.; May, C.

    1989-01-01

    This book concentrates on the routine radiographic examinations commonly performed. It details the wide variety of examinations possible and their place in initial learning and in the radiology department as references for those occasions when an unusual examination is requested. This book provides information ranging from basic terminology to skeletal positioning to special procedures. Positions are discussed and supplemented with a picture of a patient, the resulting radiograph, and a labeled diagram. Immobilization and proper shielding of the patient are also shown

  14. Position encoder

    International Nuclear Information System (INIS)

    Goursky, Vsevolod

    1975-01-01

    A circuitry for deriving the quotient of signal delivered by position-sensitive detectors is described. Digital output is obtained in the form of 10- to 12-bit words. Impact position may be determined with 0.25% accuracy when the dynamic range of the energy signal is less 1:10, and 0.5% accuracy when the dynamic range is 1:20. The division requires an average time of 5μs for 10-bit words

  15. Position encoder

    International Nuclear Information System (INIS)

    Goursky, V.

    1975-05-01

    This paper describes circuitry for deriving the quotient of signals delivered by position-sensitive detectors. Digital output is obtained in the form of 10 to 12 bit words. Impact position may be determined with 0.25% accuracy when the dynamic range of the energy signal is less than 1:10, and 0.5% accuracy when the dynamic range is 1:20. The division requires an average time of 5μs for 10-bit words [fr

  16. Positional games

    CERN Document Server

    Hefetz, Dan; Stojaković, Miloš; Szabó, Tibor

    2014-01-01

    This text serves as a thorough introduction to the rapidly developing field of positional games. This area constitutes an important branch of combinatorics, whose aim it is to systematically develop an extensive mathematical basis for a variety of two-player perfect information games. These range from such popular games as Tic-Tac-Toe and Hex to purely abstract games played on graphs and hypergraphs. The subject of positional games is strongly related to several other branches of combinatorics such as Ramsey theory, extremal graph and set theory, and the probabilistic method. These notes cover a variety of topics in positional games, including both classical results and recent important developments. They are presented in an accessible way and are accompanied by exercises of varying difficulty, helping the reader to better understand the theory. The text will benefit both researchers and graduate students in combinatorics and adjacent fields.

  17. Analysis of cocoa flavanols and procyanidins (DP 1-10) in cocoa-containing ingredients and products by rapid resolution liquid chromatography: single-laboratory validation.

    Science.gov (United States)

    Machonis, Philip R; Jones, Matthew A; Kwik-Uribe, Catherine

    2014-01-01

    Recently, a multilaboratory validation (MLV) of AOAC Official Method 2012.24 for the determination of cocoa flavanols and procyanidins (CF-CP) in cocoa-based ingredients and products determined that the method was robust, reliable, and transferrable. Due to the complexity of the CF-CP molecules, this method required a run time exceeding 1 h to achieve acceptable separations. To address this issue, a rapid resolution normal phase LC method was developed, and a single-laboratory validation (SLV) study conducted. Flavanols and procyanidins with a degree of polymerization (DP) up to 10 were eluted in 15 min using a binary gradient applied to a diol stationary phase, detected using fluorescence detection, and reported as a total sum of DP 1-10. Quantification was achieved using (-)-epicatechin-based relative response factors for DP 2-10. Spike recovery samples and seven different types of cocoa-based samples were analyzed to evaluate the accuracy, precision, LOD, LOQ, and linearity of the method. The within-day precision of the reported content for the samples was 1.15-5.08%, and overall precision was 3.97-13.61%. Spike-recovery experiments demonstrated recoveries of over 98%. The results of this SLV were compared to those previously obtained in the MLV and found to be consistent. The translation to rapid resolution LC allowed for an 80% reduction in analysis time and solvent usage, while retaining the accuracy and reliability of the original method. The savings in both cost and time of this rapid method make it well-suited for routine laboratory use.

  18. BİNA İÇİ MEKANLARDA PROFIBUS-DP AĞI ÜZERİNDEN DİNAMİK AYDINLATMA DENETİMİ

    Directory of Open Access Journals (Sweden)

    Cemal YILMAZ

    2007-01-01

    Full Text Available Bu çalışmada, bina içi mekanlarda Profıbus-DP ağı üzerinden dinamik aydınlatma denetimi gerçekleştirilmiştir. Aydınlatmanın istenilen aydınlık düzeylerine göre ayarlanabilmesi için dimlenebilir armatürler kullanılmıştır. Bina içerisinde aydınlık düzeyini ölçen algılayıcılardan gelen bilgiler Profibus-DP ağı üzerinden merkezi denetim ünitesine aktarılmaktadır. Burada yapılan değerlendirme sonucuna göre aydınlatma armatürlerine kontrol sinyalleri yine Profibus-DP ağı üzerinden gönderilerek denetim sağlanmıştır. Tasarımda aydınlatma kontrolünün uzaktan denetimi ile enerjinin optimum kullanımı sağlanmıştır. Ayrıca günün değişik saatlerinde meydana gelen ışık değişimine göre aydınlatma seviyesi ayarlanarak sağlıklı bir aydınlatma ortamı elde edilmiştir.

  19. An Evaluation of Global and Local Tensile Properties of Friction-Stir Welded DP980 Dual-Phase Steel Joints Using a Digital Image Correlation Method.

    Science.gov (United States)

    Lee, Hyoungwook; Kim, Cheolhee; Song, Jung Han

    2015-12-04

    The effect of the microstructure heterogeneity on the tensile plastic deformation characteristic of friction-stir-welded (FSW) dual-phase (DP) steel was investigated for the potential applications on the lightweight design of vehicles. Friction-stir-welded specimens with a butt joint configuration were prepared, and quasi-static tensile tests were conducted, to evaluate the tensile properties of DP980 dual-phase steels. The friction-stir welding led to the formation of martensite and a significant hardness rise in the stir zone (SZ), but the presence of a soft zone in the heat-affected zone (HAZ) was caused by tempering of the pre-existing martensite. Owing to the appearance of severe soft zone, DP980 FSW joint showed almost 93% joint efficiency with the view-point of ultimate tensile strength and relatively low ductility than the base metal (BM). The local tensile deformation characteristic of the FSW joints was also examined using the digital image correlation (DIC) methodology by mapping the global and local strain distribution, and was subsequently analyzed by mechanics calculation. It is found that the tensile deformation of the FSW joints is highly heterogeneous, leading to a significant decrease in global ductility. The HAZ of the joints is the weakest region where the strain localizes early, and this localization extends until fracture with a strain near 30%, while the strain in the SZ and BM is only 1% and 4%, respectively. Local constitutive properties in different heterogeneous regions through the friction-stir-welded joint was also briefly evaluated by assuming iso-stress conditions. The local stress-strain curves of individual weld zones provide a clear indication of the heterogeneity of the local mechanical properties.

  20. Researcher Positioning

    DEFF Research Database (Denmark)

    Khawaja, Iram; Mørck, Line Lerche

    2009-01-01

    involvement by the researcher, which challenges traditional perspectives onresearch and researcher positioning. A key point in this regard is the importance ofconstant awareness of and reflection on the multiple ways in which one's positioningas a researcher influences the research process. Studying the other...

  1. Position detector

    International Nuclear Information System (INIS)

    Hayakawa, Toshifumi.

    1985-01-01

    Purpose: To enable to detect the position of an moving object in a control rod position detector, stably in a digital manner at a high accuracy and free from the undesired effects of circumstantial conditions such as the reactor temperature. Constitution: Coils connected in parallel with each other are disposed along the passage of a moving object and variable resistors and relays are connected in series with each of the coils respectively. Light emitting diodes is connected in series with the contacts of the respective relays. The resistance value of the variable resistors are adjusted depending on the changes in the circumstantial conditions and temperature distribution upon carrying out the positional detection. When the object is inserted into a coils, the relevant relay is deenergized, by which the relay contacts are closed to light up the diode. In the same manner, as the object is successively inserted into the coils, the diodes are lighted-up successively thereby enabling highly accurate and stable positional detection in a digital manner, free from the undesired effects of the circumstantial conditions. (Horiuchi, T.)

  2. Analysis of 2H(rvec d,p)3H reaction by the four-body Faddeev-Yakubovsky equations

    International Nuclear Information System (INIS)

    Uzu, E.; Oryu, S.; Tanifuji, M.

    1995-01-01

    Very low energy 2 H(rvec d,p) 3 H reactions are investigated by using the four-body Fadeev-Yakubovsky integral equations. The adopted potential is the Ernst-Shakin-Thaler's separable expansion of the Paris potential or the PEST potential. The [3+1] and the [2+2] sub-amplitudes are given by the Hilbert-Schmidt rank-2, rank-3 and rank-4 separable expansion. The calculated total cross section, differential cross section, and tensor analyzing powers at 30keV-90keV are in very good agreement with the experimental data. copyright 1995 American Institute of Physics

  3. Exploring Natural Products from the Biodiversity of Pakistan for Computational Drug Discovery Studies: Collection, Optimization, Design and Development of A Chemical Database (ChemDP).

    Science.gov (United States)

    Mirza, Shaher Bano; Bokhari, Habib; Fatmi, Muhammad Qaiser

    2015-01-01

    Pakistan possesses a rich and vast source of natural products (NPs). Some of these secondary metabolites have been identified as potent therapeutic agents. However, the medicinal usage of most of these compounds has not yet been fully explored. The discoveries for new scaffolds of NPs as inhibitors of certain enzymes or receptors using advanced computational drug discovery approaches are also limited due to the unavailability of accurate 3D structures of NPs. An organized database incorporating all relevant information, therefore, can facilitate to explore the medicinal importance of the metabolites from Pakistani Biodiversity. The Chemical Database of Pakistan (ChemDP; release 01) is a fully-referenced, evolving, web-based, virtual database which has been designed and developed to introduce natural products (NPs) and their derivatives from the biodiversity of Pakistan to Global scientific communities. The prime aim is to provide quality structures of compounds with relevant information for computer-aided drug discovery studies. For this purpose, over 1000 NPs have been identified from more than 400 published articles, for which 2D and 3D molecular structures have been generated with a special focus on their stereochemistry, where applicable. The PM7 semiempirical quantum chemistry method has been used to energy optimize the 3D structure of NPs. The 2D and 3D structures can be downloaded as .sdf, .mol, .sybyl, .mol2, and .pdb files - readable formats by many chemoinformatics/bioinformatics software packages. Each entry in ChemDP contains over 100 data fields representing various molecular, biological, physico-chemical and pharmacological properties, which have been properly documented in the database for end users. These pieces of information have been either manually extracted from the literatures or computationally calculated using various computational tools. Cross referencing to a major data repository i.e. ChemSpider has been made available for overlapping

  4. Prostaglandin D2 Receptor DP1 Antibodies Predict Vaccine-induced and Spontaneous Narcolepsy Type 1: Large-scale Study of Antibody Profiling

    Directory of Open Access Journals (Sweden)

    Helle Sadam

    2018-03-01

    Full Text Available Background: Neuropathological findings support an autoimmune etiology as an underlying factor for loss of orexin-producing neurons in spontaneous narcolepsy type 1 (narcolepsy with cataplexy; sNT1 as well as in Pandemrix influenza vaccine-induced narcolepsy type 1 (Pdmx-NT1. The precise molecular target or antigens for the immune response have, however, remained elusive. Methods: Here we have performed a comprehensive antigenic repertoire analysis of sera using the next-generation phage display method - mimotope variation analysis (MVA. Samples from 64 children and adolescents were analyzed: 10 with Pdmx-NT1, 6 with sNT1, 16 Pandemrix-vaccinated, 16 H1N1 infected, and 16 unvaccinated healthy individuals. The diagnosis of NT1 was defined by the American Academy of Sleep Medicine international criteria of sleep disorders v3. Findings: Our data showed that although the immunoprofiles toward vaccination were generally similar in study groups, there were also striking differences in immunoprofiles between sNT1 and Pdmx-NT1 groups as compared with controls. Prominent immune response was observed to a peptide epitope derived from prostaglandin D2 receptor (DP1, as well as peptides homologous to B cell lymphoma 6 protein. Further validation confirmed that these can act as true antigenic targets in discriminating NT1 diseased along with a novel epitope of hemagglutinin of H1N1 to delineate exposure to H1N1. Interpretation: We propose that DP1 is a novel molecular target of autoimmune response and presents a potential diagnostic biomarker for NT1. DP1 is involved in the regulation of non-rapid eye movement (NREM sleep and thus alterations in its functions could contribute to the disturbed sleep regulation in NT1 that warrants further studies. Together our results also show that MVA is a helpful method for finding novel peptide antigens to classify human autoimmune diseases, possibly facilitating the design of better therapies. Keywords: Narcolepsy type 1

  5. Genetic polymorphisms in HLA-DP and STAT4 are associated with IgA nephropathy in a Southwest Chinese population

    OpenAIRE

    Yang, Bin; Zhang, Junlong; Liu, Xinle; Huang, Zhuochun; Su, Zhenzhen; Liao, Yun; Wang, Lanlan

    2018-01-01

    IgA nephropathy (IgAN) is the most common chronic glomerular disease worldwide. Genetic factors are thought to be crucial in the pathogenesis of IgAN. However, few data are available on the relationship between human leucocyte antigen (HLA) and signal transducer and activator of transcription 4 (STAT4) polymorphisms and IgAN susceptibility in the Chinese population. Therefore, we examined HLA-DP/DQ and STAT4 polymorphisms (rs3077, rs9277535, rs7453920 and rs7574865) in a total of 630 subjects...

  6. [Results transferability on RXL, ARX, X-Pand, BN2 (Dade Behring) and modular DP (Roche Diagnostics) analysers: application to component assays of fibrotest and Actitest].

    Science.gov (United States)

    Imbert-Bismut, F; Messous, D; Raoult, A; Poynard, T; Bertrand, J J; Marie, P A; Louis, V; Audy, C; Thouy, J M; Hainque, B; Piton, A

    2005-01-01

    The follow up of patients with chronic liver diseases and the data from multicentric clinical studies are affected by the variability of assay results for the same parameter between the different laboratories. Today, the main objective in clinical chemistry throughout the world is to harmonise the assay results between the laboratories after the confirmation of their traceability, in relation to defined reference systems. In this context, the purpose of our study was to verify the homogeneity of haptoglobin, apolipoprotein A1, total bilirubin, GGT activity, ALAT activity results, which are combined in Fibrotest and Actitest, between Dimension Analysers RXL, ARX and X-PAND (Dade Behring Society). Moreover, we verified the transferability of Fibrotest and Actitest results between the RXL, and either the BN2 (haptoglobin and apolipoprotein A1) or the Modular DP (total bilirubin, GGT and ALAT activity concentrations). The serum samples from 150 hospitalised patients were analysed on the different analysers. Specific protein assays were calibrated using solutions standardised against reference material on Dimension and BN2 analysers. Total bilirubin assays were performed by a diazoreaction on Dimension and Modular DP analysers. The GGT and ALAT activity measurements on the Dimension analysers were performed in accordance with the reference methods defined by the International Federation of Clinical Chemisty and Laboratory Medicine (IFCC). On the Modular, enzyme activity measurements were performed according to the Szasz method (L-gamma- glutamyl-4-nitroanilide as substrate) modified by Persijn and van der Slik (L-gamma- glutamyl-3-carboxy- 4-nitroanilide as substrat) for GGT and according to the IFCC specifications for ALAT. The methods of enzymatic activity measurement were calibrated on the Modular only. Liver fibrosis and necroinflammatory activity indices were determined using calculation algorithms, after having adjusted each component's result of Fibrotest and

  7. Maintaining positive

    OpenAIRE

    Gheorghe Gh. IONESCU; Adina Letitia NEGRUSA

    2004-01-01

    Maintaining positive work-force relationships includes in effective labor-management relations and making appropriate responses to current employee issues. Among the major current employee issues are protection from arbitrary dismissal, drug and alcohol abuse, privacy rights and family maters and they impact work. In our paper we discus two problems: first, the meanings of industrial democracy; second, the three principal operational concepts of industrial democracy (1) industrial democracy t...

  8. Patients with Duchenne muscular dystrophy are significantly shorter than those with Becker muscular dystrophy, with the higher incidence of short stature in Dp71 mutated subgroup.

    Science.gov (United States)

    Matsumoto, Masaaki; Awano, Hiroyuki; Lee, Tomoko; Takeshima, Yasuhiro; Matsuo, Masafumi; Iijima, Kazumoto

    2017-11-01

    Duchenne and Becker muscular dystrophy (DMD/BMD) are caused by mutations in the dystrophin gene and are characterized by severe and mild progressive muscle wasting, respectively. Short stature has been reported as a feature of DMD in the Western hemisphere, but not yet confirmed in Orientals. Height of young BMD has not been fully characterized. Here, height of ambulant and steroid naive Japanese 179 DMD and 42 BMD patients between 4 and 10 years of age was retrospectively examined using height standard deviation score (SDS). The mean height SDS of DMD was -1.08 SD that was significantly smaller than normal (p < 0.001), indicating short stature of Japanese DMD. Furthermore, the mean height SDS of BMD was -0.27 SD, suggesting shorter stature than normal. Remarkably, the mean height SDS of DMD was significantly smaller than that of BMD (p < 0.0001). In DMD higher incidence of short stature (height SDS < -2.5 SD) was observed in Dp71 subgroup having mutations in dystrophin exons 63-79 than others having mutations in exons 1-62 (27.8% vs. 7.5%, p = 0.017). These suggested that height is influenced by dystrophin in not only DMD but also BMD and that dystrophin Dp71 has a role in height regulation. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Concurrent Label-Free Mass Spectrometric Analysis of Dystrophin Isoform Dp427 and the Myofibrosis Marker Collagen in Crude Extracts from mdx-4cv Skeletal Muscles

    Science.gov (United States)

    Murphy, Sandra; Zweyer, Margit; Mundegar, Rustam R.; Henry, Michael; Meleady, Paula; Swandulla, Dieter; Ohlendieck, Kay

    2015-01-01

    The full-length dystrophin protein isoform of 427 kDa (Dp427), the absence of which represents the principal abnormality in X-linked muscular dystrophy, is difficult to identify and characterize by routine proteomic screening approaches of crude tissue extracts. This is probably related to its large molecular size, its close association with the sarcolemmal membrane, and its existence within a heterogeneous glycoprotein complex. Here, we used a careful extraction procedure to isolate the total protein repertoire from normal versus dystrophic mdx-4cv skeletal muscles, in conjunction with label-free mass spectrometry, and successfully identified Dp427 by proteomic means. In contrast to a considerable number of previous comparative studies of the total skeletal muscle proteome, using whole tissue proteomics we show here for the first time that the reduced expression of this membrane cytoskeletal protein is the most significant alteration in dystrophinopathy. This agrees with the pathobiochemical concept that the almost complete absence of dystrophin is the main defect in Duchenne muscular dystrophy and that the mdx-4cv mouse model of dystrophinopathy exhibits only very few revertant fibers. Significant increases in collagens and associated fibrotic marker proteins, such as fibronectin, biglycan, asporin, decorin, prolargin, mimecan, and lumican were identified in dystrophin-deficient muscles. The up-regulation of collagen in mdx-4cv muscles was confirmed by immunofluorescence microscopy and immunoblotting. Thus, this is the first mass spectrometric study of crude tissue extracts that puts the proteomic identification of dystrophin in its proper pathophysiological context. PMID:28248273

  10. Demonstration of L-band DP-QPSK transmission over FSO and fiber channels employing InAs/InP quantum-dash laser source

    KAUST Repository

    Shemis, M.A.

    2017-11-23

    The next generation of optical access communication networks that support 100 Gbps and beyond, require advances in modulation schemes, spectrum utilization, new transmission bands, and efficient devices, particularly laser diodes. In this paper, we investigated the viability of new-class of InAs/InP Quantum-dash laser diode (Qdash-LD) exhibiting multiple longitudinal light modes in the L-band to carry high-speed data rate for access network applications. We exploited external and self injection-locking techniques on Qdash-LD to generate large number of stable and tunable locked modes, and compared them. To stem the capability of each locked mode as a potential subcarrier, data transmission is carried out over two mediums; single mode fiber (SMF) and free space optics (FSO) to emulate real deployment scenarios of optical networks. The results showed that with external-injection locking (EIL), an error-free transmission of 100 Gbps dual polarization quadrature phase shift keying (DP-QPSK) signal is demonstrated over 10 km SMF and 4 m indoor FSO channels, with capability of reaching up to 128 Gbps, demonstrated under back-to-back (BTB) configuration. On the other hand, using self-injection locking (SIL) scheme, a successful data transmission of 64 Gbps and 128 Gbps DP-QPSK signal over 20 km SMF and 10 m indoor FSO links, respectively, is achieved.

  11. Demonstration of L-band DP-QPSK transmission over FSO and fiber channels employing InAs/InP quantum-dash laser source

    Science.gov (United States)

    Shemis, M. A.; Khan, M. T. A.; Alkhazraji, E.; Ragheb, A. M.; Esmail, M. A.; Fathallah, H.; Qureshi, K. K.; Alshebeili, S.; Khan, M. Z. M.

    2018-03-01

    The next generation of optical access communication networks that support 100 Gbps and beyond, require advances in modulation schemes, spectrum utilization, new transmission bands, and efficient devices, particularly laser diodes. In this paper, we investigated the viability of new-class of InAs/InP Quantum-dash laser diode (Qdash-LD) exhibiting multiple longitudinal light modes in the L-band to carry high-speed data rate for access network applications. We exploited external and self injection-locking techniques on Qdash-LD to generate large number of stable and tunable locked modes, and compared them. To stem the capability of each locked mode as a potential subcarrier, data transmission is carried out over two mediums; single mode fiber (SMF) and free space optics (FSO) to emulate real deployment scenarios of optical networks. The results showed that with external-injection locking (EIL), an error-free transmission of 100 Gbps dual polarization quadrature phase shift keying (DP-QPSK) signal is demonstrated over 10 km SMF and 4 m indoor FSO channels, with capability of reaching up to 128 Gbps, demonstrated under back-to-back (BTB) configuration. On the other hand, using self-injection locking (SIL) scheme, a successful data transmission of 64 Gbps and 128 Gbps DP-QPSK signal over 20 km SMF and 10 m indoor FSO links, respectively, is achieved.

  12. Laser, tungsten inert gas, and metal active gas welding of DP780 steel: Comparison of hardness, tensile properties and fatigue resistance

    International Nuclear Information System (INIS)

    Lee, Jeong Hun; Park, Sung Hyuk; Kwon, Hyuk Sun; Kim, Gyo Sung; Lee, Chong Soo

    2014-01-01

    Highlights: • We report the mechanical properties of DP780 steel welded by three methods. • The size of the welded zone increases with heat input (MAG > TIG > laser). • The hardness of the welded zone increases with cooling rate (laser > TIG > MAG). • Tensile and fatigue properties are strongly dependent on welding method. • Crack initiation sites depend on the microstructural features of the welded zone. - Abstract: The microstructural characteristics, tensile properties and low-cycle fatigue properties of a dual-phase steel (DP780) were investigated following its joining by three methods: laser welding, tungsten inert gas (TIG) welding, and metal active gas (MAG) welding. Through this, it was found that the size of the welded zone increases with greater heat input (MAG > TIG > laser), whereas the hardness of the weld metal (WM) and heat-affected zone (HAZ) increases with cooling rate (laser > TIG > MAG). Consequently, laser- and TIG-welded steels exhibit higher yield strength than the base metal due to a substantially harder WM. In contrast, the strength of MAG-welded steel is reduced by a broad and soft WM and HAZ. The fatigue life of laser-and TIG-welded steel was similar, with both being greater than that of MAG-welded steel; however, the fatigue resistance of all welds was inferior to that of the non-welded base metal. Finally, crack initiation sites were found to differ depending on the microstructural characteristics of the welded zone, as well as the tensile and cyclic loading

  13. Implication of climate change on urban drainage systems of Chicago and Mainstream and DesPlaines (MS/DP) Tunnel and Reservoir Plan (TARP)

    Science.gov (United States)

    Luo, H.; Schmidt, A.; Garcia, M. H.; Oberg, N.

    2016-12-01

    The impact of changing climate patterns and rainfall extremes on sewer system and river basin has been brought to attention to the researchers worldwide. In 1972, the Metropolitan Water Reclamation District of Greater Chicago (MWRDGC) adopted the Tunnel and Reservoir Plan (TARP) to address combined sewer overflow (CSO) pollution and flooding problems in the Chicago land area. The hydrosystem laboratory in University of Illinois at Urbana-Champaign developed a series of numerical models accordingly to analyze the complex hydraulic behavior of the as-built TARP system. Due to the interconnected nature of City of Chicago sewer network and MS/DP TARP system, a tightly coupled hydrological and hydraulic model MetroFlow was developed to facilitate such analysis by integrating previous developed models. This study utilized MetroFlow to predict the hydrologic/hydraulic response of the system for a set of pre-determined design and historical storm events. Accordingly, combined sewer overflows (CSO) of Chicago combined sewer system and MS/DP TARP system were evaluated under current and future weather scenarios. The total CSOs from TARP system can be considered as urban point pollution source to the surrounding receiving bodies, hence the potential impact of climate change on CSO fluxes is essential reference to wastewater infrastructure design and operations of the hydraulic regulating structures under storm events to mitigate predicted risks.

  14. An Experiment of Robust Parallel Algorithm for the Eigenvalue problem of a Multigroup Neutron Diffusion based on modified FETI-DP

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Jonghwa [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-05-15

    Parallelization of Monte Carlo simulation is widely adpoted. There are also several parallel algorithms developed for the SN transport theory using the parallel wave sweeping algorithm and for the CPM using parallel ray tracing. For practical purpose of reactor physics application, the thermal feedback and burnup effects on the multigroup cross section should be considered. In this respect, the domain decomposition method(DDM) is suitable for distributing the expensive cross section calculation work. Parallel transport code and diffusion code based on the Raviart-Thomas mixed finite element method was developed. However most of the developed methods rely on the heuristic convergence of flux and current at the domain interfaces. Convergence was not attained in some cases. Mechanical stress computation community has also work on the DDM to solve the stress-strain equation using the finite element methods. The most successful domain decomposition method in terms of robustness is FETI-DP. We have modified the original FETI-DP to solve the eigenvalue problem for the multigroup diffusion problem in this study.

  15. Genetic polymorphisms in HLA-DP and STAT4 are associated with IgA nephropathy in a Southwest Chinese population.

    Science.gov (United States)

    Yang, Bin; Zhang, Junlong; Liu, Xinle; Huang, Zhuochun; Su, Zhenzhen; Liao, Yun; Wang, Lanlan

    2018-01-23

    IgA nephropathy (IgAN) is the most common chronic glomerular disease worldwide. Genetic factors are thought to be crucial in the pathogenesis of IgAN. However, few data are available on the relationship between human leucocyte antigen (HLA) and signal transducer and activator of transcription 4 (STAT4) polymorphisms and IgAN susceptibility in the Chinese population. Therefore, we examined HLA-DP/DQ and STAT4 polymorphisms (rs3077, rs9277535, rs7453920 and rs7574865) in a total of 630 subjects including 140 IgAN and 490 healthy controls in Chinese. There were significant associations between IgAN patients and healthy controls in the allele frequency of rs3077, rs9277535 and rs7574865. In addition, the genotypes of rs3077, rs9277535 and rs7574865 were also significantly associated with IgAN under recessive models. Moreover, the haplotypes block AAG, AGG, GAG and GGA in the HLA gene significantly correlated with the risk of IgAN. This is the first study demonstrating the significant associations of SNP rs3077, rs9277535 and rs7574865 and the haplotypes in the HLA gene with the risk of IgAN in a Southwest Chinese population. This research provides a new insight into the significant relationship between HLA-DP and STAT4 polymorphisms and the susceptibility to IgAN.

  16. Robust H∞ Control of Neutral System with Time-Delay for Dynamic Positioning Ships

    Directory of Open Access Journals (Sweden)

    Dawei Zhao

    2015-01-01

    Full Text Available Due to the input time-delay existing in most thrust systems of the ships, the robust H∞ controller is designed for the ship dynamic positioning (DP system with time-delay. The input delay system is turned to a neutral time-delay system by a state-derivative control law. The less conservative result is derived for the neutral system with state-derivative feedback by the delay-decomposition approach and linear matrix inequality (LMI. Finally, the numerical simulations demonstrate the asymptotic stability and robustness of the controller and verify that the designed DP controller is effective in the varying environment disturbances of wind, waves, and ocean currents.

  17. Mushroom acidic glycosphingolipid induction of cytokine secretion from murine T cells and proliferation of NK1.1 α/β TCR-double positive cells in vitro

    International Nuclear Information System (INIS)

    Nozaki, Hirofumi; Itonori, Saki; Sugita, Mutsumi; Nakamura, Kimihide; Ohba, Kiyoshi; Suzuki, Akemi; Kushi, Yasunori

    2008-01-01

    Interferon (IFN)-γ and interleukin (IL)-4 regulate many types of immune responses. Here we report that acidic glycosphingolipids (AGLs) of Hypsizigus marmoreus and Pleurotus eryngii induced secretion of IFN- γ and IL-4 from T cells in a CD11c-positive cell-dependent manner similar to that of α-galactosylceramide (α-GalCer) and isoglobotriaosylceramide (iGb3), although activated T cells by AGLs showed less secretion of cytokine than those activated by α-GalCer. In addition, stimulation of these mushroom AGLs induced proliferation of NK1.1 α/β TCR-double positive cells in splenocytes. Administration of a mixture of α-GalCer and AGLs affected the stimulation of α-GalCer and generally induced a subtle Th1 bias for splenocytes but induced an extreme Th2 bias for thymocytes. These results suggested that edible mushroom AGLs contribute to immunomodulation

  18. VALIDATION OF THE MECG-DP-NS-01 MONITOR IN OSCILLOMETRY AND AUSCULTATION MODES IN CHILDREN AND ADOLESCENTS, ACCORDING TO ESH-IP2, BHS AND AAMI PROTOCOLS

    Directory of Open Access Journals (Sweden)

    S. I. Fedorova

    2015-01-01

    Full Text Available Background: High blood pressure in childhood and adolescence is associated with a 2 to 3-fold increase of the risk of arterial hypertension. According to the Russian guidelines, only devices that have been tested by international protocols can be used for the main diagnostic method of arterial hypertension in children and adolescents, i.e. ambulatory 24-hour blood pressure monitoring.Aim: To validate the MECG-DP-NS-01 upper arm BP monitor in oscillometry and auscultation modes within the “Soyuz” complex, in children and adolescents aged from 5 to 15 years according to the international protocol of the European Society of Hypertension (ESH from 2010 (ESH-IP2, the protocol of the British Hypertension Society (BHS from 1993 and the standard of the Association for the Advancement of Medical Instrumentation (AAMI.Materials and methods: We recruited 99 children and adolescents (49 male, 50 female aged from 5 to 15 years (33 children, from 5 to 7 years, 33, from 8 to 11 years, 33, from 12 to 15 years. Expert and device blood pressure measurements were performed in each patient according to the protocols.Results: The MECG-DP-NS-01 upper arm blood pressure monitor was validated and its accuracy in blood pressure measurement in children and adolescents according to ESH-IP2, BHS and AAMI protocols confirmed. According to BHS 1993 protocol, its accuracy corresponded to A/A both in the oscillometry and auscultation modes.Conclusion: According to ESH-IP2, BHS and AAMI protocols, MEGC-DP-NS-01 within the “Soyuz” complex could be recommended for 24-hour ambulatory blood pressure monitoring in children and adolescents aged from 5 to 15 years, both in the oscillometry and auscultation modes. According to the Declaration of blood pressure Measuring Device Equivalence signed by the manufacturer for the devices MDP-NS-02s “Voshod” and MEGCDPNS-01, and to the equivalence criteria for blood pressure measuring devices, the results of testing and its

  19. Technical aspects of typing for HLA-DP alleles using allele-specific DNA in vitro amplification and sequence-specific oligonucleotide probes. Detection of single base mismatches

    DEFF Research Database (Denmark)

    Fugger, L; Morling, N; Ryder, L P

    1990-01-01

    The polymerase chain reaction (PCR) is an effective method for in vitro DNA amplification which combined with probing with synthetic oligonucleotides can be used for, e.g., HLA-typing. We have studied the technical aspects of HLA-DP typing with the technique. DNA from mononuclear nucleated cells...... was extracted with either a simple salting out method or phenol/chloroform. Both DNAs could be readily used for PCR. The MgC2 concentration of the PCR buffer and the annealing temperature of the thermal cycle of the PCR were the two most important variables. The MgCl2 concentration and the temperature must...... be carefully titrated for each primer pair in the PCR. The influence of mismatches between the primer and the DNA template were studied and we found that, by using primers differing only from each other at the 3' end, cross-amplification of closely homologous alleles could be avoided. Thus, single base...

  20. Results on pi /sup -/p backward elastic scattering and search for pi /sup -/p to dp at 9 and 12 GeV/c

    CERN Document Server

    Jacholkowski, A; Bouquet, B; D'Almagne, B; De Rosny, G; Ferrer, A; Fleury, P; Lahellec, A; Petroff, P; Richard, F; Rivet, P; Roudeau, P; Rougé, A; Six, J; Treille, D; Yoshida, H

    1977-01-01

    From an experiment done with the CERN Omega spectrometer, triggered by a fast forward proton device, results on the differential cross section d sigma /du for pi /sup -/p backward elastic scattering are presented. The d sigma /du distribution agrees with an Ae/sup BU/ law. The compilation of existing results shows a discrepancy between results but the (d sigma /du)/sub u=0/ data fit perfectly on s/sup 2 alpha 0-2/ dependence, as predicted by a single Delta /sub delta / Regge trajectory exchange. A search for the reaction pi /sup -/p to dp, with a fast forward deuteron, which can be produced by a double- baryon exchange mechanism, gives cross-section upper limits of approximately 1% of the backward elastic cross section. (10 refs).

  1. Measurement of the B Meson Differential Cross Section dσ/dpT in p bar p Collisions at √s=1.8 TeV

    International Nuclear Information System (INIS)

    Abe, F.; Albrow, M.G.; Amendolia, S.R.; Amidei, D.; Antos, J.; Anway-Wiese, C.; Apollinari, G.; Areti, H.; Atac, M.; Auchincloss, P.; Azfar, F.; Azzi, P.; Bacchetta, N.; Badgett, W.; Bailey, M.W.; Bao, J.; de Barbaro, P.; Barbaro-Galtieri, A.; Barnes, V.E.; Barnett, B.A.; Bartalini, P.; Bauer, G.; Baumann, T.; Bedeschi, F.; Behrends, S.; Belforte, S.; Bellettini, G.; Bellinger, J.; Benjamin, D.; Benlloch, J.; Bensinger, J.; Benton, D.; Beretvas, A.; Berge, J.P.; Bertolucci, S.; Bhatti, A.; Biery, K.; Binkley, M.; Bird, F.; Bisello, D.; Blair, R.E.; Blocker, C.; Bodek, A.; Bokhari, W.; Bolognesi, V.; Bortoletto, D.; Boswell, C.; Boulos, T.; Brandenburg, G.; Bromberg, C.; Buckley-Geer, E.; Budd, H.S.; Burkett, K.; Busetto, G.; Byon-Wagner, A.; Byrum, K.L.; Cammerata, J.; Campagnari, C.; Campbell, M.; Caner, A.; Carithers, W.; Carlsmith, D.; Castro, A.; Cen, Y.; Cervelli, F.; Chao, H.Y.; Chapman, J.; Cheng, M.; Chiarelli, G.; Chikamatsu, T.; Chiou, C.N.; Christofek, L.; Cihangir, S.; Clark, A.G.; Cobal, M.; Contreras, M.; Conway, J.; Cooper, J.; Cordelli, M.; Couyoumtzelis, C.; Crane, D.; Cunningham, J.D.; Daniels, T.; DeJongh, F.; Delchamps, S.; Dell'Agnello, S.; Dell'Orso, M.; Demortier, L.; Denby, B.; Deninno, M.; Derwent, P.F.; Devlin, T.; Dickson, M.; Dittmann, J.R.; Donati, S.; Drucker, R.B.; Dunn, A.; Einsweiler, K.; Elias, J.E.; Ely, R.; Engels, E. Jr.; Eno, S.; Errede, D.; Errede, S.; Fan, Q.; Farhat, B.; Fiori, I.; Flaugher, B.; Foster, G.W.; Franklin, M.; Frautschi, M.; Freeman, J.; Friedman, J.; Fry, A.; Fuess, T.A.; Fukui, Y.; Funaki, S.; Gagliardi, G.; Galeotti, S.; Gallinaro, M.; Garfinkel, A.F.; Geer, S.; Gerdes, D.W.; Giannetti, P.; Giokaris, N.; Giromini, P.; Gladney, L.; Glenzinski, D.; Gold, M.; Gonzalez, J.; Gordon, A.; Goshaw, A.T.; Goulianos, K.; Grassmann, H.; Grewal, A.; Groer, L.; Grosso-Pilcher, C.; Haber, C.; Hahn, S.R.; Hamilton, R.; Handler, R.; Hans, R.M.; Hara, K.; Harral, B.; Harris, R.M.; Hauger, S.A.; Hauser, J.; Hawk, C.

    1995-01-01

    This paper presents the first direct measurement of the B meson differential cross section dσ/dp T in p bar p collisions at √s=1.8 TeV using a sample of 19.3±0.7 pb --1 accumulated by the Collider Detector at Fermilab. The cross section is measured in the central rapidity region |y| T (B)>6.0 GeV/c by fully reconstructing the B meson decays B + →J/ψK + and B 0 →J/ψK *0 (892), where J/ψ→μ + μ - and K *0 →K + π - . A comparison is made to the theoretical QCD prediction calculated at next-to-leading order

  2. OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice.

    Science.gov (United States)

    Li, Fengcheng; Xie, Guosheng; Huang, Jiangfeng; Zhang, Ran; Li, Yu; Zhang, Miaomiao; Wang, Yanting; Li, Ao; Li, Xukai; Xia, Tao; Qu, Chengcheng; Hu, Fan; Ragauskas, Arthur J; Peng, Liangcai

    2017-09-01

    Genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. Since cellulose synthase (CESA) gene was first identified, several dozen CESA mutants have been reported, but almost all mutants exhibit the defective phenotypes in plant growth and development. In this study, the rice (Oryza sativa) Osfc16 mutant with substitutions (W481C, P482S) at P-CR conserved site in CESA9 shows a slightly affected plant growth and higher biomass yield by 25%-41% compared with wild type (Nipponbare, a japonica variety). Chemical and ultrastructural analyses indicate that Osfc16 has a significantly reduced cellulose crystallinity (CrI) and thinner secondary cell walls compared with wild type. CESA co-IP detection, together with implementations of a proteasome inhibitor (MG132) and two distinct cellulose inhibitors (Calcofluor, CGA), shows that CESA9 mutation could affect integrity of CESA4/7/9 complexes, which may lead to rapid CESA proteasome degradation for low-DP cellulose biosynthesis. These may reduce cellulose CrI, which improves plant lodging resistance, a major and integrated agronomic trait on plant growth and grain production, and enhances biomass enzymatic saccharification by up to 2.3-fold and ethanol productivity by 34%-42%. This study has for the first time reported a direct modification for the low-DP cellulose production that has broad applications in biomass industries. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  3. Characterization of a novel Dp71 dystrophin-associated protein complex (DAPC) present in the nucleus of HeLa cells: Members of the nuclear DAPC associate with the nuclear matrix

    International Nuclear Information System (INIS)

    Fuentes-Mera, Lizeth; Rodriguez-Munoz, Rafael; Gonzalez-Ramirez, Ricardo; Garcia-Sierra, Francisco; Gonzalez, Everardo; Mornet, Dominique; Cisneros, Bulmaro

    2006-01-01

    Dystrophin is an essential component in the assembly and maintenance of the dystrophin-associated protein complex (DAPC), which includes members of the dystroglycan, syntrophin, sarcoglycan and dystrobrevin protein families. Distinctive complexes have been described in the cell membrane of different tissues and cultured cells. In this work, we report the identification and characterization of a novel DAPC present in the nuclei of HeLa cells, which contains dystrophin Dp71 as a key component. Using confocal microscopy and cell fractionation analyses, we found the presence of Dp71, β-sarcoglycan, β-dystroglycan, α- and β-syntrophin, α1- and β-dystrobrevin and nNOS in the nuclei of HeLa cells. Furthermore, we demonstrated by co-immunoprecipitation experiments that most of these proteins form a complex in the nuclear compartment. Next, we analyze the possible association of the nuclear DAPC with the nuclear matrix. We found the presence of Dp71, β-dystroglycan, nNOS, β-sarcoglycan, α/β syntrophin, α1-dystrobrevin and β-dystrobrevin in the nuclear matrix protein fractions and in situ nuclear matrix preparations from HeLa cells. Moreover, we found that Dp71, β-dystroglycan and β-dystrobrevin co-immunoprecipitated with the nuclear matrix proteins lamin B1 and actin. The association of members of the nuclear DAPC with the nuclear matrix indicates that they may work as scaffolding proteins involved in nuclear architecture

  4. CTD, salinity, temperature, oxygen, and depth data for Cruise DP01 from the R/V Point Sur in the Viosca Knoll, Gulf of Mexico, 2015-05-01 to 2015-05-08 (NCEI Accession 0142203)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Deep water sampling of in situ seawater and associated fauna (cruise DP01, May 1-8, 2015) aboard the R/V Point Sur for an area encompassing roughly 28°N to 29°N...

  5. Differences in acid tolerance between Bifidobacterium breve BB8 and its acid-resistant derivative B. breve BB8dpH, revealed by RNA-sequencing and physiological analysis.

    Science.gov (United States)

    Yang, Xu; Hang, Xiaomin; Tan, Jing; Yang, Hong

    2015-06-01

    Bifidobacteria are common inhabitants of the human gastrointestinal tract, and their application has increased dramatically in recent years due to their health-promoting effects. The ability of bifidobacteria to tolerate acidic environments is particularly important for their function as probiotics because they encounter such environments in food products and during passage through the gastrointestinal tract. In this study, we generated a derivative, Bifidobacterium breve BB8dpH, which displayed a stable, acid-resistant phenotype. To investigate the possible reasons for the higher acid tolerance of B. breve BB8dpH, as compared with its parental strain B. breve BB8, a combined transcriptome and physiological approach was used to characterize differences between the two strains. An analysis of the transcriptome by RNA-sequencing indicated that the expression of 121 genes was increased by more than 2-fold, while the expression of 146 genes was reduced more than 2-fold, in B. breve BB8dpH. Validation of the RNA-sequencing data using real-time quantitative PCR analysis demonstrated that the RNA-sequencing results were highly reliable. The comparison analysis, based on differentially expressed genes, suggested that the acid tolerance of B. breve BB8dpH was enhanced by regulating the expression of genes involved in carbohydrate transport and metabolism, energy production, synthesis of cell envelope components (peptidoglycan and exopolysaccharide), synthesis and transport of glutamate and glutamine, and histidine synthesis. Furthermore, an analysis of physiological data showed that B. breve BB8dpH displayed higher production of exopolysaccharide and lower H(+)-ATPase activity than B. breve BB8. The results presented here will improve our understanding of acid tolerance in bifidobacteria, and they will lead to the development of new strategies to enhance the acid tolerance of bifidobacterial strains. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. FEBEX-DP. Dismantling the ''full-scale engineered barrier experiment'' after 18 years of operation at the Grimsel Test Site, Switzerland

    Energy Technology Data Exchange (ETDEWEB)

    Kober, Florian; Gaus, Irina [Nagra, Wettingen (Switzerland)

    2015-07-01

    dismantling project (FEBEX-DP) are: - Characterization of the key physical properties (e.g., density, water content) of the barrier and their distribution, - Characterization of corrosion and microbiological processes on instruments and coupons resulting from evolving redox conditions and saturation states, including gas analysis, - Characterization macro- and micro level studies of mineralogical interactions at material interfaces (e.g., cement-bentonite or iron-bentonite, rock-bentonite), - Assessment of sensor performance, - Further increasing understanding of the thermo-hydro-mechanical (THM) and thermo-hydro-chemical (THC) processes through integration of monitoring and dismantling results. An intensive laboratory program will be conducted in 2015/2016 in order to achieve the outlined main objectives. It includes extensive mineralogical, chemical and biological investigations of the buffer and the related interfaces. It will be accompanied by pre- and post dismantling modeling efforts. Unique data are expected after completing one of the longest running 1:1 in-situ EBS experiment under continued heating and natural saturation conditions. It will further consolidate the EBS knowledge and will act as a benchmark for major coupled modeling codes. The dismantling project is set-up as an international project with partners from Europe, Asia and North America. Further information can are under http://www.grimsel.com/gts-phase-vi/febex-dp/febex-dp-introduction.

  7. Rationale and design of the DP-TRANSFERS project: diabetes prevention-transferring findings from European research to society in Catalonia.

    Science.gov (United States)

    Costa, Bernardo; Castell, Conxa; Cos, Xavier; Solé, Claustre; Mestre, Santiago; Canela, Marta; Boquet, Antoni; Cabré, Joan-Josep; Barrio, Francisco; Flores-Mateo, Gemma; Ferrer-Vidal, Daniel; Lindström, Jaana

    2016-04-27

    Compelling evidence has been accumulated to support the effectiveness of intensive lifestyle intervention in delaying progression to Type 2 diabetes even in people identified as being at high risk determined by the Finnish diabetes risk score. The DE-PLAN-CAT project (diabetes in Europe-prevention using lifestyle, physical activity and nutritional intervention-Catalonia) evidenced that intensive lifestyle intervention was feasible and cost-effective on a short scale in real-life primary care settings, at least over 4 years. However, transferring such lifestyle interventions to society remains the major challenge of research in the field of diabetes prevention. The derived DP-TRANSFERS (diabetes prevention-transferring findings from European research to society) is a large scale national programme aimed at translating a tailored lifestyle intervention to the maximum of primary care centres where feasible through a core proposal agreed with all the partners. The method is built upon a 3-step (screening, intervention and follow-up) real-life, community-wide structure on the basis of a dual intensity lifestyle intervention (basic and continuity modules) and supported by a 4-channel transfer strategy (institutional relationships, facilitators' workshops, collaborative groupware and programme WEB page). Participation will initially cover nine health departments (7 million inhabitants) through nine coordinating centres located in metropolitan (3.2 million), semi-urban (2.9 million) and rural (0.9 million) areas from which it is expected accessing 25 % of all primary care settings, equivalent to 90 associated centres (1.6-1.8 million people) with an estimate of 0.32 million participants aged 45-75 years at high risk of future development of diabetes. To ascertain sustainability, effect, satisfaction and quality of the translation programme statistical analyses will be performed from both the entire population (facilitators and participants) and a stratified

  8. Analyzing power T20 measurement of backscattering d.p. vector in the Δ resonance excitation range and theoretical analysis of this reaction

    International Nuclear Information System (INIS)

    Boudard, A.

    1983-12-01

    We have measured the analysing power T 20 in the backward elastic scattering d.p. for 16 energies of the deuteron from 300 MeV to 2300 MeV. This is the region of the observed bump in the backward excitation function of the cross section. This bump is usually thought to be a signature of a Δ(3/2,3/2 + ) dynamically excited in the intermediate state. We have also measured Ay and Ayy from 70 0 to 180 0 for Tsub(d)=1200 MeV. we have compared both T 20 and the backward cross section with a coherent sum between direct neutron exchange and Δ excitation by intermediate exchanges of π and rho mesons. The overall shape of the cross section is reproduced. Unlike the earlier measurements from Argonne, there is a deep minimum in T 20 at Tsub(d)=600 MeV, in agreement with the predictions of direct exchange models. However, an additional structure producing a second minimum at Tsub(d)=1400 MeV (√S=3240 MeV) is never reproduced by our calculations. This suggests either that refinements in the Δ treatment are needed or that a new reaction mechanism (resonance) takes place in that region [fr

  9. Evaluation of commercial repository capacity for the disposal of defense high-level waste. Comments and responses for DOE/DP--0020

    International Nuclear Information System (INIS)

    1985-12-01

    The Nuclear Waste Policy Act of 1982 (Public Law 97-425) requires that the President evaluate the use of disposal capacity at one or more repositories to be developed for permanent disposal of civilian spent nuclear fuel and high-level radioactive waste for the disposal of defense high-level radioactive waste. The Department of Energy prepared a report titled ''An Evaluation of Commercial Repository Capacity for the Disposal of Defense High-Level Waste,'' DOE/DP-0020, to provide input for the President's evaluation. The report constituted the Department's input and recommendation to be considered by the President in making his evaluation. Although not required by the Act, the Department made the July 1984 draft of the report available to the general public for review and comment in order to increase public awareness, and develop a public record on the issue of disposal of defense high-level waste. Over 400 copies of the draft report were distributed. Thirty comment letters containing over 400 comments were received from representatives of states, localities, and Indian tribes, federal agencies, organizations representing utilities, public interest groups, individual utilities, and private citizens. All letters were reviewed and considered. Where appropriate, changes were made in the final report reflecting the comments received

  10. An Exploratory Study of Cost Engineering in Axiomatic Design: Creation of the Cost Model Based on an FR-DP Map

    Science.gov (United States)

    Lee, Taesik; Jeziorek, Peter

    2004-01-01

    Large complex projects cost large sums of money throughout their life cycle for a variety of reasons and causes. For such large programs, the credible estimation of the project cost, a quick assessment of the cost of making changes, and the management of the project budget with effective cost reduction determine the viability of the project. Cost engineering that deals with these issues requires a rigorous method and systematic processes. This paper introduces a logical framework to a&e effective cost engineering. The framework is built upon Axiomatic Design process. The structure in the Axiomatic Design process provides a good foundation to closely tie engineering design and cost information together. The cost framework presented in this paper is a systematic link between the functional domain (FRs), physical domain (DPs), cost domain (CUs), and a task/process-based model. The FR-DP map relates a system s functional requirements to design solutions across all levels and branches of the decomposition hierarchy. DPs are mapped into CUs, which provides a means to estimate the cost of design solutions - DPs - from the cost of the physical entities in the system - CUs. The task/process model describes the iterative process ot-developing each of the CUs, and is used to estimate the cost of CUs. By linking the four domains, this framework provides a superior traceability from requirements to cost information.

  11. Benign positional vertigo

    Science.gov (United States)

    Vertigo - positional; Benign paroxysmal positional vertigo; BPPV: dizziness- positional ... Benign positional vertigo is also called benign paroxysmal positional vertigo (BPPV). It is caused by a problem in the inner ear. ...

  12. Analysis of environment, safety, and health (ES{ampersand}H) management systems for Department of Energy (DOE) Defense Programs (DP) facilities

    Energy Technology Data Exchange (ETDEWEB)

    Neglia, A. V., LLNL

    1998-03-01

    each system with respect to ES&H, the hazard of concern, and any limitations with respect to the TQM categories. A summary evaluation and explanation of each of the systems is provided in Section 4 of the paper. Several other ES&H systems were reviewed in preparation of the paper, but were not specifically included as a system in this matrix. Only those ES&H management systems that are potentially applicable to DOE Defense Program sites were included as part of the matrix comparison. A description of other ES&H management systems that were evaluated, but not specifically incorporated in this matrix comparison, are provided in Attachment 3 entitled, `Other ES&H Management Systems Reviewed.` In the past, it has been difficult integrating ES&H into work planning for several reasons. One barrier to this integration has been the complexity caused by the existence of several `stove pipe` ES&H systems. By analyzing the unique elements of the various ES&H systems, as well as their strengths and limitations, and their similarities and differences, it is envisioned that this paper will aid in facilitating the integration of ES&H into work planning. This paper was developed by the Office of Defense Programs (DP-45) and all questions or comments should be directed to Anthony Neglia of that office at (301) 903-3531 or Anthony.Neglia@dp.doe.gov.

  13. The effect of simultaneous exposure of HEMn-DP and HEMn-LP melanocytes to nicotine and UV-radiation on the cell viability and melanogenesis

    International Nuclear Information System (INIS)

    Delijewski, Marcin; Wrześniok, Dorota; Beberok, Artur; Rok, Jakub; Otręba, Michał; Buszman, Ewa

    2016-01-01

    Nicotine is a main compound of tobacco plants and may affect more than a billion people all over the world that are permanently exposed to nicotine from cigarettes, various forms of smoking cessation therapies, electronic cigarettes or second-hand smoke. It is known that nicotine forms complexes with melanin what may lead to accumulation of this alkaloid in tissues of living organisms containing the pigment. This may affect the viability of cells and process of melanin biosynthesis that takes place in melanocytes. Although UV radiation is known to be a particular inductor of melanin biosynthesis, its simultaneous effect with nicotine on this process as well as the viability of human cells containing melanin have not been assessed so far. The aim of this study was to examine the simultaneous impact of nicotine and UV radiation on viability and melanogenesis in cultured normal human melanocytes dark (HEMn-DP) and light (HEMn-LP) pigmented. Nicotine together with UV radiation induced concentration-dependent loss in melanocytes viability. The higher cell loss was observed in dark pigmented melanocytes in comparison to light pigmented cells. Simultaneous exposure of cells to nicotine and UV radiation also caused changes in melanization process in both tested cell lines. The data suggest that simultaneous exposure of melanocytes to nicotine and UV radiation up-regulates melanogenesis and affects cell viability. Observed processes are more pronounced in dark pigmented cells. - Highlights: • Nicotine and UVA induced concentration-dependent loss in melanocytes viability. • Nicotine and UVA modulated melanization process in melanocytes. • Changes in viability and melanization were more pronounced in dark pigmented cells.

  14. The effect of simultaneous exposure of HEMn-DP and HEMn-LP melanocytes to nicotine and UV-radiation on the cell viability and melanogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Delijewski, Marcin; Wrześniok, Dorota; Beberok, Artur; Rok, Jakub; Otręba, Michał; Buszman, Ewa, E-mail: ebuszman@sum.edu.pl

    2016-11-15

    Nicotine is a main compound of tobacco plants and may affect more than a billion people all over the world that are permanently exposed to nicotine from cigarettes, various forms of smoking cessation therapies, electronic cigarettes or second-hand smoke. It is known that nicotine forms complexes with melanin what may lead to accumulation of this alkaloid in tissues of living organisms containing the pigment. This may affect the viability of cells and process of melanin biosynthesis that takes place in melanocytes. Although UV radiation is known to be a particular inductor of melanin biosynthesis, its simultaneous effect with nicotine on this process as well as the viability of human cells containing melanin have not been assessed so far. The aim of this study was to examine the simultaneous impact of nicotine and UV radiation on viability and melanogenesis in cultured normal human melanocytes dark (HEMn-DP) and light (HEMn-LP) pigmented. Nicotine together with UV radiation induced concentration-dependent loss in melanocytes viability. The higher cell loss was observed in dark pigmented melanocytes in comparison to light pigmented cells. Simultaneous exposure of cells to nicotine and UV radiation also caused changes in melanization process in both tested cell lines. The data suggest that simultaneous exposure of melanocytes to nicotine and UV radiation up-regulates melanogenesis and affects cell viability. Observed processes are more pronounced in dark pigmented cells. - Highlights: • Nicotine and UVA induced concentration-dependent loss in melanocytes viability. • Nicotine and UVA modulated melanization process in melanocytes. • Changes in viability and melanization were more pronounced in dark pigmented cells.

  15. Forming limit curves of DP600 determined in high-speed Nakajima tests and predicted by two different strain-rate-sensitive models

    Science.gov (United States)

    Weiß-Borkowski, Nathalie; Lian, Junhe; Camberg, Alan; Tröster, Thomas; Münstermann, Sebastian; Bleck, Wolfgang; Gese, Helmut; Richter, Helmut

    2018-05-01

    Determination of forming limit curves (FLC) to describe the multi-axial forming behaviour is possible via either experimental measurements or theoretical calculations. In case of theoretical determination, different models are available and some of them consider the influence of strain rate in the quasi-static and dynamic strain rate regime. Consideration of the strain rate effect is necessary as many material characteristics such as yield strength and failure strain are affected by loading speed. In addition, the start of instability and necking depends not only on the strain hardening coefficient but also on the strain rate sensitivity parameter. Therefore, the strain rate dependency of materials for both plasticity and the failure behaviour is taken into account in crash simulations for strain rates up to 1000 s-1 and FLC can be used for the description of the material's instability behaviour at multi-axial loading. In this context, due to the strain rate dependency of the material behaviour, an extrapolation of the quasi-static FLC to dynamic loading condition is not reliable. Therefore, experimental high-speed Nakajima tests or theoretical models shall be used to determine the FLC at high strain rates. In this study, two theoretical models for determination of FLC at high strain rates and results of experimental high-speed Nakajima tests for a DP600 are presented. One of the theoretical models is the numerical algorithm CRACH as part of the modular material and failure model MF GenYld+CrachFEM 4.2, which is based on an initial imperfection. Furthermore, the extended modified maximum force criterion considering the strain rate effect is also used to predict the FLC. These two models are calibrated by the quasi-static and dynamic uniaxial tensile tests and bulge tests. The predictions for the quasi-static and dynamic FLC by both models are presented and compared with the experimental results.

  16. Effect of PGD2 on middle meningeal artery and mRNA expression profile of L-PGD2 synthase and DP receptors in trigeminovascular system and other pain processing structures in rat brain

    DEFF Research Database (Denmark)

    Sekeroglu, Aysegül; Jacobsen, Julie Mie; Jansen-Olesen, Inger

    2017-01-01

    Background Prostaglandins (PGs), particularly prostaglandin D2 (PGD2), E2 (PGE2), and I2 (PGI2), are considered to play a role in migraine pain. In humans, infusion of PGD2 causes lesser headache as compared to infusion of PGE2 and PGI2. Follow-up studies in rats have shown that infusion of PGE2...... and PGI2 dilate the middle meningeal artery (MMA), and mRNA for PGE2 and PGI2 receptors is present in rat trigeminovascular system (TVS) and in the brain structures associated with pain. In the present study, we have characterized the dilatory effect of PGD2 on rat MMA and studied the relative m...... tested tissues. DP1 receptor mRNA was expressed maximally in trigeminal ganglion (TG) and in cervical dorsal root ganglion (DRG). Conclusions High expression of DP1 mRNA in the TG and DRG suggest that PGD2 might play a role in migraine pathophysiology. Activation of the DP1 receptor in MMA was mainly...

  17. Position automatic determination technology

    International Nuclear Information System (INIS)

    1985-10-01

    This book tells of method of position determination and characteristic, control method of position determination and point of design, point of sensor choice for position detector, position determination of digital control system, application of clutch break in high frequency position determination, automation technique of position determination, position determination by electromagnetic clutch and break, air cylinder, cam and solenoid, stop position control of automatic guide vehicle, stacker crane and automatic transfer control.

  18. Positive Psychology: Positive Emotions and Emotional Intelegence

    OpenAIRE

    Miloseva, Lence

    2008-01-01

    The paper focuses on the and emotional intelligence. We try to answer on some questions regarding the role which positive emotions have in our life’s. The broaden-and-build theory (Fredrickson, 1998; 2001) predicts that positive emotions are useful in several ways. They guide present behavior, by broadening one’s attention and cognition, setting the stage for creative, explorative, and innovative pursuits. As well, positive emotions build personal and social resources to help individuals achi...

  19. Comparison of broiler performance when fed diets containing event DP-3O5423-1, nontransgenic near-isoline control, or commercial reference soybean meal, hulls, and oil.

    Science.gov (United States)

    McNaughton, J; Roberts, M; Smith, B; Rice, D; Hinds, M; Sanders, C; Layton, R; Lamb, I; Delaney, B

    2008-12-01

    DP-3Ø5423-1 (305423) is a genetically modified soybean that was produced by biolistic insertion of the gm-fad2-1 gene fragment and gm-hra genes into the germline of soybean seeds. Expression of gm-fad2-1 results in greater concentrations of oleic acid (18:1) by suppressing expression of the endogenous FAD2-1 gene, which encodes an n-6 fatty acid desaturase enzyme that catalyzes desaturation of 18:1 to linoleic acid (18:2). The GM-HRA protein expressed by the gm-hra gene is a modified version of the soybean acetolactate synthase enzyme that is used as a selectable marker during transformation. A 42-d feeding trial was conducted with broiler chickens to compare the nutritional performance of 305423 soybeans with nontransgenic soybeans. Diets were prepared using processed fractions (meal, hulls, and oil) from 305423 soybean plants. For comparison, additional diets were produced with soybean fractions obtained from a nontransgenic near-isoline (control) and nontransgenic commercial Pioneer brand varieties (93B86, 93B15, and 93M40). Diets were fed to Ross x Cobb broilers (n = 120/group, 50% male and 50% female) in 3 phases. Starter, grower, and finisher diets contained 26.5, 23, and 21.5% soybean meal, respectively. Soybean hulls and oil were added at 1.0 and 0.5%, respectively, across all diets in each phase. No statistically significant differences were observed in growth performance (BW, mortality, feed efficiency), organ yield (liver and kidney), or carcass yield (breast, thigh, leg, wing, and abdominal fat) variables between broilers consuming diets prepared with isolated fractions from 305423 or near-isoline control soybean. Additionally, all performance and carcass variables from control and 305423 soybean treatment groups fell within tolerance intervals constructed for each response variable using data from broilers fed diets prepared with reference soybean fractions. Based on the results from this study, it was concluded that 305423 soybeans were nutritionally

  20. Want Positive Behavior? Use Positive Language

    Science.gov (United States)

    Wood, Chip; Freeman-Loftis, Babs

    2012-01-01

    Positive adult language is the professional use of words and tone of voice to enable students to learn in an engaged, active way. This includes learning social skills. To guide children toward choosing and maintaining positive behaviors, adults need to carefully choose the words and tone of voice used when speaking to them. Learning to use…

  1. Benign positional vertigo - aftercare

    Science.gov (United States)

    Vertigo - positional - aftercare; Benign paroxysmal positional vertigo - aftercare; BPPV - aftercare; Dizziness - positional vertigo ... Your health care provider may have treated your vertigo with the Epley maneuver . These are head movements ...

  2. Study of the reactions 9Be (d, a0) 7Li, 9Be (d, a1) 7Li*, 9Be (dt)8Be and 9Be (dp0) 10Be from 300 to 1000 keV

    International Nuclear Information System (INIS)

    Bertrand, F.; Grenier, G.; Pornet, J.

    1968-01-01

    We present the excitation curves, the angular distributions and the total cross-sections for the reactions: 9 Be (d α 0 ) 7 Li, 9 Be (d α 1 ) 7 Li, 9 Be (d, t) 8 Be, 9 Be (dp 0 ) 10 Be, in the energy range from 300 keV to 1 MeV. Our results are in good agreement with the few studies already carried out. In order that the results be presented in absolute values, we have normalized them with those of BIGGERSTAFF. (author) [fr

  3. NetMHCIIpan-3.0, a common pan-specific MHC class II prediction method including all three human MHC class II isotypes, HLA-DR, HLA-DP and HLA-DQ

    DEFF Research Database (Denmark)

    Karosiene, Edita; Rasmussen, Michael; Blicher, Thomas

    2013-01-01

    Major histocompatibility complex class II (MHCII) molecules play an important role in cell-mediated immunity. They present specific peptides derived from endosomal proteins for recognition by T helper cells. The identification of peptides that bind to MHCII molecules is therefore of great importa......MHCIIpan-3.0 method is the first pan-specific predictor covering all HLA class II molecules with known sequences including HLA-DR, HLA-DP, and HLA-DQ. The NetMHCpan-3.0 method is available at http://www.cbs.dtu.dk/services/NetMHCIIpan-3.0....

  4. Multi-technology positioning

    CERN Document Server

    Lohan, Elena-Simona; Wymeersch, Henk; Seco-Granados, Gonzalo; Nykänen, Ossi

    2017-01-01

    This book provides an overview of positioning technologies, applications and services in a format accessible to a wide variety of readers. Readers who have always wanted to understand how satellite-based positioning, wireless network positioning, inertial navigation, and their combinations work will find great value in this book. Readers will also learn about the advantages and disadvantages of different positioning methods, their limitations and challenges. Cognitive positioning, adding the brain to determine which technologies to use at device runtime, is introduced as well. Coverage also includes the use of position information for Location Based Services (LBS), as well as context-aware positioning services, designed for better user experience. • Brings understanding of positioning technology to readers from a variety of disciplines • Reviews multiple techniques, providing insight on the pros, cons and challenges related to each • Designed to be a tutorial on basic principles, avoiding unnecessary de...

  5. Navicular bone position determined by positional MRI

    DEFF Research Database (Denmark)

    Hansen, Philip; Johannsen, Finn E; Hangaard, Stine

    2016-01-01

    -scanner). Scanning was performed in supine and standing position, respectively. Two radiologists evaluated the images in a blinded manner. Reliability and agreement were assessed by calculation of intraclass correlation coefficient (ICC) and 95 % limits of agreement as a percentage of the mean (LOA%). RESULTS...

  6. DPL-1 DP, LIN-35 Rb and EFL-1 E2F act with the MCD-1 zinc-finger protein to promote programmed cell death in Caenorhabditis elegans.

    Science.gov (United States)

    Reddien, Peter W; Andersen, Erik C; Huang, Michael C; Horvitz, H Robert

    2007-04-01

    The genes egl-1, ced-9, ced-4, and ced-3 play major roles in programmed cell death in Caenorhabditis elegans. To identify genes that have more subtle activities, we sought mutations that confer strong cell-death defects in a genetically sensitized mutant background. Specifically, we screened for mutations that enhance the cell-death defects caused by a partial loss-of-function allele of the ced-3 caspase gene. We identified mutations in two genes not previously known to affect cell death, dpl-1 and mcd-1 (modifier of cell death). dpl-1 encodes the C. elegans homolog of DP, the human E2F-heterodimerization partner. By testing genes known to interact with dpl-1, we identified roles in cell death for four additional genes: efl-1 E2F, lin-35 Rb, lin-37 Mip40, and lin-52 dLin52. mcd-1 encodes a novel protein that contains one zinc finger and that is synthetically required with lin-35 Rb for animal viability. dpl-1 and mcd-1 act with efl-1 E2F and lin-35 Rb to promote programmed cell death and do so by regulating the killing process rather than by affecting the decision between survival and death. We propose that the DPL-1 DP, MCD-1 zinc finger, EFL-1 E2F, LIN-35 Rb, LIN-37 Mip40, and LIN-52 dLin52 proteins act together in transcriptional regulation to promote programmed cell death.

  7. Pretty Easy Pervasive Positioning

    DEFF Research Database (Denmark)

    Hansen, Rene; Wind, Rico; Jensen, Christian Søndergaard

    2009-01-01

    With the increasing availability of positioning based on GPS, Wi-Fi, and cellular technologies and the proliferation of mobile devices with GPS, Wi-Fi and cellular connectivity, ubiquitous positioning is becoming a reality. While offerings by companies such as Google, Skyhook, and Spotigo render...... positioning possible in outdoor settings, including urban environments with limited GPS coverage, they remain unable to offer accurate indoor positioning. We will demonstrate a software infrastructure that makes it easy for anybody to build support for accurate Wi-Fi based positioning in buildings. All...... that is needed is a building with Wi-Fi coverage, access to the building, a floor plan of the building, and a Wi-Fi enabled device. Specifically, we will explain the software infrastructure and the steps that must be completed to obtain support for positioning. And we will demonstrate the positioning obtained...

  8. Positive criminology in practice.

    Science.gov (United States)

    Ronel, Natti; Segev, Dana

    2014-11-01

    The discourse regarding offender rehabilitation has been criticized by various scholars who have claimed that reducing negative causes and managing risk will not automatically prompt positive human development and elements that are associated with desistance. Positive criminology is an innovative concept that challenges the common preoccupation with negative elements, by placing emphasis on human encounters and forces of inclusion that are experienced positively by target individuals and that can promote crime desistance. However, as the concept is relatively new, there are still no guiding principles for the practice of positive criminology that could direct research and the criminal justice system. This article attempts to fill that gap by providing principles that could be practiced by criminal justice personnel and examples of different interventions that reflect positive criminology. The article also provides ideological explanations for adopting the concept of positive criminology in practice. © The Author(s) 2013.

  9. Positive and negative regulation of V(D)J recombination by the E2A proteins.

    Science.gov (United States)

    Bain, G; Romanow, W J; Albers, K; Havran, W L; Murre, C

    1999-01-18

    A key feature of B and T lymphocyte development is the generation of antigen receptors through the rearrangement and assembly of the germline variable (V), diversity (D), and joining (J) gene segments. However, the mechanisms responsible for regulating developmentally ordered gene rearrangements are largely unknown. Here we show that the E2A gene products are essential for the proper coordinated temporal regulation of V(D)J rearrangements within the T cell receptor (TCR) gamma and delta loci. Specifically, we show that E2A is required during adult thymocyte development to inhibit rearrangements to the gamma and delta V regions that normally recombine almost exclusively during fetal thymocyte development. The continued rearrangement of the fetal Vgamma3 gene segment in E2A-deficient adult thymocytes correlates with increased levels of Vgamma3 germline transcripts and increased levels of double-stranded DNA breaks at the recombination signal sequence bordering Vgamma3. Additionally, rearrangements to a number of Vgamma and Vdelta gene segments used predominantly during adult development are significantly reduced in E2A-deficient thymocytes. Interestingly, at distinct stages of T lineage development, both the increased and decreased rearrangement of particular Vdelta gene segments is highly sensitive to the dosage of the E2A gene products, suggesting that the concentration of the E2A proteins is rate limiting for the recombination reaction involving these Vdelta regions.

  10. 77 FR 26562 - Mobile Offshore Drilling Unit Dynamic Positioning Guidance

    Science.gov (United States)

    2012-05-04

    ... questions on viewing the docket, call Renee V. Wright, Program Manager, Docket Operations, telephone 202-366... operators are encouraged to voluntarily report to the Coast Guard reactive changes of DP status from ``green...

  11. Den positive psykologis metoder

    DEFF Research Database (Denmark)

    Andersen, Frans Ørsted; Mørck, Line Lerche; Nissen, Poul Erik

    En antologi der giver en introduktion til en række af de metoder der anvendes til forskning, assessment, test, udviklingsarbejde og intervention indenfor den positive psykologi.......En antologi der giver en introduktion til en række af de metoder der anvendes til forskning, assessment, test, udviklingsarbejde og intervention indenfor den positive psykologi....

  12. Modern management positions

    Directory of Open Access Journals (Sweden)

    Jovanović Petar M.

    2015-01-01

    Full Text Available In this paper we analyze contemporary managerial positions such as program manager, project portfolio manager, crisis manager and others. The idea is to promote managerial positions in Serbia, which is quite unjustifiably undervalued, primarily because of the lack of knowledge in the field of management and other issues related to management education.

  13. Positioning and locking apparatus

    Science.gov (United States)

    Hayward, M.L.; Harper, W.H.

    1985-06-19

    A positioning and locking apparatus including a fixture having a rotatable torque ring provided with a plurality of cam segments for automatically guiding a container into a desired location within the fixture. Rotation of the ring turns the container into a final position in pressure sealing relation against a hatch member.

  14. Positioning health professional identity

    DEFF Research Database (Denmark)

    Lund, Ole; Krogh Christensen, Mette; Mørcke, Anne Mette

    2017-01-01

    Drawing on positioning theory, the purpose of this paper is to characterize the activities and positions of students and supervisors at workplaces and on-campus skills training sites across the higher health professional educations of medicine, sports science, and nursing. Furthermore, the study ...... explored the impact of work-based learning (WBL) and skills training on students’ personal professional identity development....

  15. Phantom position dependence

    International Nuclear Information System (INIS)

    Thorson, M.R.; Endres, G.W.R.

    1981-01-01

    Sensitivity of the Hanford dosimeter response to its position relative to the phantom and the neutron source has always been recognized. A thorough investigation was performed to quantify dosimeter response according to: (a) dosimeter position on phantom, (b) dosimeter distance from phantom, and (c) angular relationship of dosimeter relative to neutron source and phantom. Results were obtained for neutron irradiation at several different energies

  16. Position display device

    International Nuclear Information System (INIS)

    Nishizawa, Yukio.

    1974-01-01

    Object: To provide a device capable of easily and quickly reading mutual mounting relations of control bodies such as control rods mounted on a nuclear reactor and positions to which the control bodies are driven. Structure: A scanning circuit is provided to scan positions of controllably mounted control bodies such as control rods. Values detected by scanning the positions are converted into character signals according to the values and converted into preranked color signals. The character signals and color signals are stored in a memory circuit by synchronous signals in synchronism with the scanning in the scanning circuit. Outputs of the memory circuit are displayed by a display unit such as a color Braun tube in accordance with the synchronous signals to provide color representations according to positions to which control bodies are driven in the same positional relation as the mounting of the control bodies. (Kamimura, M.)

  17. Review of Positional Nystagmus

    Directory of Open Access Journals (Sweden)

    José Ignacio BENITO-OREJAS

    2018-01-01

    Full Text Available Introduction and objective: Any change of position of the head (dynamic or static can trigger a nystagmus or increase the spontaneous. This nystagmus we call it positional. We intend to review in this chapter its definition, clinical features, etiology and the way of exploring it. Method: narrative review. Results: Positional vertigo is the most frequent vestibular disorder, and most of the times of peripheral cause. Characteristics of nystagmus or its association with other neurological symptoms are sufficient grounds to request a magnetic resonance, which will provide additional information on very few occasions. Discussion: The study of the positional nystagmus as a procedure to rule out central pathology has lost much of its relevance. Signs that Nylén defined mixed findings peripheral and central, making complex classification. Conclusion: Regardless of how to bring the patient change position (very slow or fast, if it appears an atypical nystagmus should rule out a central origin.

  18. POSITIONING STRATEGIES DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    Shakhshir Ghassan

    2014-07-01

    Full Text Available The positioning strategy has suffered serious changes in the last few decades, being influenced by the rapid development of competition and the growing focus on specific traits belonging to the market, to the consumer or to the product. The purpose of this paper is to present the developments of theoretical positioning strategies and the orientation from more simple, product oriented strategies, to ones more oriented towards the client and with a briefer period of time. The world is moving in a much faster pace than in the past, thanks to communication development so companies are obliged to adopt more specific strategies in order for them to be effective. This essay represents a literary review presenting a documentary research within the scientific articles and strategy and positioning books. The paper begins with the analysis of company strategies and the marketing strategies in general. The first author to group the product positioning strategies is Porter with his three generic strategies. Following the development of brands and because of the lack of competitiveness in the simple generic positioning strategies, this paper has also presented the newer positioning strategies proposed by Kotler, Treacy & Wiersema, and also more complex ones such as Bowman's Strategy Clock and Blankson and Kalafatis positioning strategy based on the type of the consumer. The fast expansion of local brands in all categories has led to mistakes in positioning strategies, categories also presented in the current essay. The results of this study show that new positioning strategies are more and more based on the consumer and market segments and on the product specification - which have also evolved in the last decades. Adaptability to fast changes in the competitive market will represent the future positioning strategies.

  19. Positional Concerns and Institutions

    DEFF Research Database (Denmark)

    Landes, Xavier

    2013-01-01

    that invoking envy or subjective well-being is not fully satisfying for regulating positional concerns. More compelling reasons seem, in complement with efficiency, to be related to considerations for equality. In other words, if institutions could have strong reasons to pay attention to and regulate positional...... their implications for economics, positional concerns imply important normative dimensions. There have been presumed to be a symptom of envy, reduce people’s happiness, and create problems of social interaction or economic inefficiencies. Individuals are, for instance, prone to pick states of the world that improve...... concerns, it would be in virtue of their impact on the social product and individuals’ conditions of living....

  20. Stress Management: Positive Thinking

    Science.gov (United States)

    Healthy Lifestyle Stress management Positive thinking helps with stress management and can even improve your health. Practice overcoming negative self-talk ... with optimism is a key part of effective stress management. And effective stress management is associated with ...

  1. Den positive psykologis metoder

    DEFF Research Database (Denmark)

    Bogen introducerer til de mange metoder, som anvendes i positiv psykologi. dette sker ud fra en reflekteret tilgang, der ligeledes rummer bidrag fra kritisk psykologi med det formål at nuancere den positive psykologis forståelser...

  2. Clinical Positioning Space

    DEFF Research Database (Denmark)

    Williams, Lars Peter Hedegaard; Christensen, Mette Krogh; Rytter, Carsten

    2014-01-01

    In this article, we present a case study of residents’ clinical experiences and communication in outpatient oncology consultations. We apply positioning theory, a dynamic alternative to role theory, to investigate how oncology residents and patients situate themselves as persons with rights...... and duties. Drawing from seven qualitative interviews and six days of observation, we investigate the residents’ social positioning and their conversations with patients or supervisors. Our focus is on how (a) relational shifts in authority depend on each situation and its participants; (b) storylines...... establish acts and positions and narratively frame what participants can expect from a medical consultation viewed as a social episode; and (c) the positioning of rights and duties can lead to misunderstandings and frustrations. We conclude that residents and patients locate themselves in outpatient...

  3. Fluorescent optical position sensor

    Science.gov (United States)

    Weiss, Jonathan D.

    2005-11-15

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  4. POSITIONING STRATEGIES DEVELOPMENT

    OpenAIRE

    Shakhshir Ghassan

    2014-01-01

    The positioning strategy has suffered serious changes in the last few decades, being influenced by the rapid development of competition and the growing focus on specific traits belonging to the market, to the consumer or to the product. The purpose of this paper is to present the developments of theoretical positioning strategies and the orientation from more simple, product oriented strategies, to ones more oriented towards the client and with a briefer period of time. The world is moving in...

  5. Position measuring device

    International Nuclear Information System (INIS)

    Maeda, Kazuyuki; Takahashi, Shuichi; Maruyama, Mayumi

    1998-01-01

    The present invention provides a device capable of measuring accurate position and distance easily even at places where operator can not easily access, such as cell facilities for vitrifying radioactive wastes. Referring to a case of the vitrifying cell, an objective equipment settled in the cell is photographed by a photographing device. The image is stored in a position measuring device by way of an image input device. After several years, when the objective equipment is exchanged, a new objective equipment is photographed by a photographing device. The image is also stored in the position measuring device. The position measuring device compares the data of both of the images on the basis of pixel unit. Based on the image of the equipment before the exchange as a reference, extent of the displacement of the installation position of the equipment on the image after the exchange caused by installation error and manufacturing error is determined to decide the position of the equipment after exchange relative to the equipment before exchange. (I.S.)

  6. Last-position elimination-based learning automata.

    Science.gov (United States)

    Zhang, Junqi; Wang, Cheng; Zhou, MengChu

    2014-12-01

    An update scheme of the state probability vector of actions is critical for learning automata (LA). The most popular is the pursuit scheme that pursues the estimated optimal action and penalizes others. This paper proposes a reverse philosophy that leads to last-position elimination-based learning automata (LELA). The action graded last in terms of the estimated performance is penalized by decreasing its state probability and is eliminated when its state probability becomes zero. All active actions, that is, actions with nonzero state probability, equally share the penalized state probability from the last-position action at each iteration. The proposed LELA is characterized by the relaxed convergence condition for the optimal action, the accelerated step size of the state probability update scheme for the estimated optimal action, and the enriched sampling for the estimated nonoptimal actions. The proof of the ϵ-optimal property for the proposed algorithm is presented. Last-position elimination is a widespread philosophy in the real world and has proved to be also helpful for the update scheme of the learning automaton via the simulations of well-known benchmark environments. In the simulations, two versions of the LELA, using different selection strategies of the last action, are compared with the classical pursuit algorithms Discretized Pursuit Reward-Inaction (DP(RI)) and Discretized Generalized Pursuit Algorithm (DGPA). Simulation results show that the proposed schemes achieve significantly faster convergence and higher accuracy than the classical ones. Specifically, the proposed schemes reduce the interval to find the best parameter for a specific environment in the classical pursuit algorithms. Thus, they can have their parameter tuning easier to perform and can save much more time when applied to a practical case. Furthermore, the convergence curves and the corresponding variance coefficient curves of the contenders are illustrated to characterize their

  7. Plasma position control device

    International Nuclear Information System (INIS)

    Takase, Haruhiko.

    1987-01-01

    Purpose: To conduct position control stably to various plasmas and reduce the burden on the control coil power source. Constitution: Among the proportional, integration and differentiation controls, a proportional-differentiation control section and an integration control section are connected in parallel. Then, a signal switching circuit is disposed to the control signal input section for the proportional-differentiation control section such that either a present position of plasmas or deviation between the present plasma position and an aimed value can be selected as a control signal depending on the control procedures or the state of the plasmas. For instance, if a rapid response is required for the control, the deviation between the present plasma position and the aimed value is selected as the input signal to conduct proportional, integration and differentiation controls. While on the other hand, if it is intended to reduce the burden on the control coil power source, it is adapted such that the control signal inputted to the proportional-differentiation control section itself can select the present plasma position. (Yoshihara, H.)

  8. MHC class II+ (HLA-DP-like) cells in the cow reproductive tract: II. Immunolocalization of MHC class II+ cells in oviduct and vagina.

    Science.gov (United States)

    Eren, U; Kum, S; Sandikçi, M; Eren, V; Ilhan, F

    2009-08-01

    The aim of this study was to determine and examine the distribution of major frequency MHC II+ cells in the oviduct and vagina of cows during the oestrous and dioestrus phases. Right oviduct (ampulla, isthmus) and vaginal samples taken from a total of twenty seven multiparous cows were used. Tissue samples were processed to obtain both cryostat and paraffin sections. Sections were stained immunocytochemically using StreptABC method using a specific monoclonal antibody to MHC II+ cell population. Intra-epithelial and subepithelial areas along with lamina propria, muscularis mucosae and serosa of both ampulla and isthmus and intra-epithelial/subepithelial areas and mucosae of vagina were examined for the presence of MHC II+ cells. The density of immune positive cells was determined using a subjective scoring system. MHC II+ cells were demonstrated in all areas examined in both oestrus and dioestrus. In oestrus, the density of MHC II+ cells decreased in subepithelial areas (in between the epithelial cells and the basal membrane) of isthmus, whereas the density of immune positive cells was increased in muscularis mucosae of isthmus (P < 0.05), lamina propria and muscularis mucosae of ampulla (P < 0.05) as well as in the mucosae of vagina (P

  9. Uncharged positive electrode composition

    Science.gov (United States)

    Kaun, Thomas D.; Vissers, Donald R.; Shimotake, Hiroshi

    1977-03-08

    An uncharged positive-electrode composition contains particulate lithium sulfide, another alkali metal or alkaline earth metal compound other than sulfide, e.g., lithium carbide, and a transition metal powder. The composition along with a binder, such as electrolytic salt or a thermosetting resin is applied onto an electrically conductive substrate to form a plaque. The plaque is assembled as a positive electrode within an electrochemical cell opposite to a negative electrode containing a material such as aluminum or silicon for alloying with lithium. During charging, lithium alloy is formed within the negative electrode and transition metal sulfide such as iron sulfide is produced within the positive electrode. Excess negative electrode capacity over that from the transition metal sulfide is provided due to the electrochemical reaction of the other than sulfide alkali metal or alkaline earth metal compound.

  10. Position indication apparatus

    Energy Technology Data Exchange (ETDEWEB)

    Masuda, T

    1964-02-24

    A plurality of magnetically operated switches are spaced equally in the hollow tube of a control rod actuating mechanism. One side of each switch is connected, via a low resistance, to a corresponding tap of a low resistance voltage divider network consisting of an equivalent number of low resistance sections with the opposite side of each switch connected to a common conducting wire A. To both ends of the voltage dividing network are connected, respectively, conducting wires B and C. Wires A, B, and C are further coupled to a fuel rod position indicator comprising a voltmeter and power source external to the control rod actuating member. The control rod actuating member is adapted to slide in the hollow tube so that switches passing a position facing a magnet secured to the lower end of the actuating member are rendered closed. Hence, the position of the control rod may be read by reading the voltage off the meter.

  11. Positioning devices for patients

    International Nuclear Information System (INIS)

    Heavens, M.

    1981-01-01

    It has been suggested that it is very important to position patients reproducibly at different stages of radiotherapy treatment planning and treatment, or similar procedures. Devices have been described for positioning a patient's upper and lower thorax. This invention provides reproducible positioning for a female patient's breasts, for example in planning treatment of and treating breast tumours. The patient is placed prone, using for example an upper thorax device. A support device is placed central to and beneath her breasts to partially displace them outwards. The device may be triangular in section with one apex contacting the chest wall at the sternum. Restraining straps may be provided to hold the breasts against the support device. Means may be provided to take a healthy breast from the path of radiation through the tumour. (author)

  12. Positioning and applications

    Directory of Open Access Journals (Sweden)

    Rogowski Jerzy B.

    2015-12-01

    Full Text Available The paper presents national report of Poland for IAG on positioning and applications. The selected research presented was carried out at leading Polish research institutions and concern precise multi-GNSS satellite positioning - relative and absolute - and also GNSS-based ionosphere and troposphere modelling and studies. The research resulted in noticeable advancements in these subjects confirmed by the development of new algorithms and methods. New and improved methods of precise GNSS positioning were developed, and also GNSS metrology was studied. New advanced troposphere models were presented and tested. In particular, these models allowed testing IPW variability on regional and global scales. Also, new regional ionosphere monitoring web-based services were developed and launched.

  13. Massive Galileon positivity bounds

    Science.gov (United States)

    de Rham, Claudia; Melville, Scott; Tolley, Andrew J.; Zhou, Shuang-Yong

    2017-09-01

    The EFT coefficients in any gapped, scalar, Lorentz invariant field theory must satisfy positivity requirements if there is to exist a local, analytic Wilsonian UV completion. We apply these bounds to the tree level scattering amplitudes for a massive Galileon. The addition of a mass term, which does not spoil the non-renormalization theorem of the Galileon and preserves the Galileon symmetry at loop level, is necessary to satisfy the lowest order positivity bound. We further show that a careful choice of successively higher derivative corrections are necessary to satisfy the higher order positivity bounds. There is then no obstruction to a local UV completion from considerations of tree level 2-to-2 scattering alone. To demonstrate this we give an explicit example of such a UV completion.

  14. Nucleosome Positioning and Epigenetics

    Science.gov (United States)

    Schwab, David; Bruinsma, Robijn

    2008-03-01

    The role of chromatin structure in gene regulation has recently taken center stage in the field of epigenetics, phenomena that change the phenotype without changing the DNA sequence. Recent work has also shown that nucleosomes, a complex of DNA wrapped around a histone octamer, experience a sequence dependent energy landscape due to the variation in DNA bend stiffness with sequence composition. In this talk, we consider the role nucleosome positioning might play in the formation of heterochromatin, a compact form of DNA generically responsible for gene silencing. In particular, we discuss how different patterns of nucleosome positions, periodic or random, could either facilitate or suppress heterochromatin stability and formation.

  15. Attitude and position tracking

    CSIR Research Space (South Africa)

    Candy, LP

    2011-01-01

    Full Text Available Several applications require the tracking of attitude and position of a body based on velocity data. It is tempting to use direction cosine matrices (DCM), for example, to track attitude based on angular velocity data, and to integrate the linear...

  16. Logotherapy and positive psychology

    Directory of Open Access Journals (Sweden)

    Oscar R. Oro

    2015-09-01

    Full Text Available Psychology omitted to approach, during almost a century, the positive aspects from persons, like creativity, humor, optimism, hope, forgiveness, life meaning, and happiness. These themes are approached by Positive Psychology, with Seligman like the principal exponent. Psychology was dedicated to explore the negative aspects from human beings improving human health. Nevertheless, this pathogenic model could not prevent mental disease. Concepts of Positive Psychology have a solid antecedent in Víktor Frankl ́s studies, which is the Logotherapy founder. This allows incorporating another perspective to approach positive aspects, from a philosophical and anthropological focus. Although the ways adopted by Frank and Seligman are different, both considered main aspects of human existence. Nevertheless, they investigated in different countries (from Europe and EE.UU.; in different circumstances (concentration camps, deaths, tortures; vs. academic context; in different historical periods and different social contexts (a country that lost the war and other that gave freedom to Europeans. In this work is used the concept life sense as the focus in professional formation and psychotherapy approach. 

  17. Delegation. Position Statement. Revised

    Science.gov (United States)

    Board, Connie; Bushmiaer, Margo; Davis-Alldritt, Linda; Fekaris, Nina; Morgitan, Judith; Murphy, M. Kathleen; Yow, Barbara

    2010-01-01

    It is the position of the National Association of School Nurses (NASN) that the delegation of nursing tasks in the school setting can be a valuable tool for the school nurse, when based on the nursing definition of delegation and in compliance with state nursing regulations and guidance. Delegation in school nursing is a complex process in which…

  18. Precise Point Positioning

    DEFF Research Database (Denmark)

    Zhang, Xiaohong

    the airborne lidar system misalignment angle by automating the matching of lidar data with ground truth. Kinematic GPS positioning has been widely used, but the available commercial software systems are normally only suitable for the short or medium range kinematic baseline. However, in polar areas, airborne...

  19. Positionality at the Center

    Directory of Open Access Journals (Sweden)

    Catherine Vanner

    2015-11-01

    Full Text Available As a Western feminist supporting and researching gender equality in education in postcolonial contexts, I often wonder: Am I doing more harm than good? The privilege of my social location means that my efforts to support education in postcolonial contexts risk being patronizing, insulting, threatening, imperialist, and recolonizing. Yet neglecting and ignoring postcolonial contexts similarly reflects and reproduces a privileged position. I provide a tentative framework designed to address positionality, power, and privilege while creating an ethical research process for working in a postcolonial context. Beginning with an identification of positionality, the objectives of research, and guiding theoretical frameworks to situate the research in relation to the participants and context, I proceed to establish a methodology designed to minimize the negative effects of power and maximize participants’ empowerment. I position myself as a bricoleur, layering feminist standpoint theory and postcolonial theory, and propose the collaborative data collection and analysis techniques, with particular attention to ethical and cultural sensitivity, using a social constructivist approach to grounded theory. This article highlights the need for Western researchers to reflect upon the power dynamics of their research in postcolonial contexts and develop a strategy for conducting empowering research that prevents the misrepresentation and exploitation of participants. Observations from my doctoral thesis data collection provide examples of how these concepts were operationalized in practice as well as reflections on the disconnect between theorizing and conducting ethical research in postcolonial contexts.

  20. Immunizations. Position Statement. Revised

    Science.gov (United States)

    Bobo, Nichole; Garrett, Jennifer; Teskey, Carmen; Duncan, Kay; Strasser, Kathy; Burrows-Mezu, Alicia L.

    2015-01-01

    It is the position of the National Association of School Nurses (NASN) that immunizations are essential to primary prevention of disease from infancy through adulthood. Promotion of immunizations by the registered professional school nurse (hereinafter referred to as school nurse) is central to the public health focus of school nursing practice…

  1. Net positive energy buildings

    International Nuclear Information System (INIS)

    Romero, A.; Barreiro, E.; Sanchez Zabala, V.

    2010-01-01

    Buildings are great consumers of energy, being responsible for almost 36% of CO2 emissions in Europe. Though there are many initiatives towards the reduction of energy consumption and CO2 emissions in buildings, many of the alternatives are diminished due to a lack of a unique and holistic approach to the problem. This paper reports a new innovative concept of Positive Energy Buildings (EB+), as well as an integral methodology that covers the overall design process for achieving them. The methodology evaluates energy efficiency solutions at different scales, from building site to generation systems. An educational building design in Navarra serves as a case study to check the feasibility of the proposed methodology. The study concludes that the key to achieve a Positive Energy Building is a minimized energy demand, complemented by efficient facilities and enhanced by distributed power generation from renewable sources. (Author).

  2. Conflict, Memory, and Positioning

    DEFF Research Database (Denmark)

    Brescó, Ignacio

    2016-01-01

    The aim of this paper was to bring the dialogical and multivoiced dimension of conflict to the fore in the study of how people remember a particular event in the past. Drawing from different case studies, it contains analyses of how subjects identifying with different political actors in the Basque...... conflict adopted their respective positions, and interpretation of the conflict, and how, in light of same, they reconstruct the failed peace process that took place in 2006 between the terrorist group ETA (Euzkadi ta Azcatasuna, or Basque Country and Freedom in English) and the Spanish government. Results...... show that the positioning adopted by participants gives rise to a certain form of interpreting the conflict, which, in turn, affects how the peace process is remembered. This occurs within a particular argumentative context in which each version constitutes an implicit response to a competing...

  3. Are Parton Distributions Positive?

    CERN Document Server

    Forte, Stefano; Ridolfi, Giovanni; Altarelli, Guido; Forte, Stefano; Ridolfi, Giovanni

    1999-01-01

    We show that the naive positivity conditions on polarized parton distributions which follow from their probabilistic interpretation in the naive parton model are reproduced in perturbative QCD at the leading log level if the quark and gluon distribution are defined in terms of physical processes. We show how these conditions are modified at the next-to-leading level, and discuss their phenomenological implications, in particular in view of the determination of the polarized gluon distribution

  4. Are parton distributions positive?

    International Nuclear Information System (INIS)

    Forte, Stefano; Altarelli, Guido; Ridolfi, Giovanni

    1999-01-01

    We show that the naive positivity conditions on polarized parton distributions which follow from their probabilistic interpretation in the naive parton model are reproduced in perturbative QCD at the leading log level if the quark and gluon distribution are defined in terms of physical processes. We show how these conditions are modified at the next-to-leading level, and discuss their phenomenological implications, in particular in view of the determination of the polarized gluon distribution

  5. Spatial Search, Position Papers

    OpenAIRE

    Center for Spatial Studies, UCSB

    2014-01-01

    The Spatial Search specialist meeting in Santa Barbara (December 2014) brought together 35 academic and industry representatives from computational, geospatial, and cognitive sciences with interest in focused discussions on the development of an interdisciplinary research agenda to advance spatial search from scientific and engineering viewpoints. The position papers from participants represent the shared expertise that guided discussions and the formulation of research questions about proces...

  6. Barebacking and sexual position

    OpenAIRE

    Grundy-Bowers, M.; Hardy, S.; McKeown, E.

    2015-01-01

    Bareback sex continues to fuel the HIV epidemic among men who have sex with men but despite the fact that much academic attention has been focused on the sexual behaviour of this population few authors have considered the significance of sexual position. In order to explore this relatively under-examined factor, interviews were conducted with 13 HIV-negative and unknown status gay men who had recently engaged in bareback sex. Using Interpretative Phenomenological Analysis and through the lens...

  7. The strategic research positioning:

    DEFF Research Database (Denmark)

    Viala, Eva Silberschmidt

    to provide new insights into ‘immigrant’ parents’ perspective on home/school partnership in Denmark. The majority of the immigrant parents came from non-Western countries, and they had already been ‘labelled’ difficult in terms of home/school partnership. This calls for what I call ‘strategic research...... positioning’, meaning critical reflections about the relationship and power balance between the researcher and the researched. The paper focus' on challenges and dilemmas linked to this position....

  8. Review of HRP Positions

    Energy Technology Data Exchange (ETDEWEB)

    Center for Reliability Studies

    2007-01-01

    The Department of Energy (DOE) Human Reliability Program (HRP), published as 10 CFR Part 712, is currently being reviewed and revised to address concerns identified during its implementation. Although these ''page changes'' primarily incorporate clarification of terms and language, the following discussion relates to broadening the definition of positions that require HRP certification that is found in {section}712.10.

  9. Position indicating device

    International Nuclear Information System (INIS)

    Fellchenfeld, M.M.; Connors, G.R.

    1987-01-01

    This patent describes a position indicating device for producing an indication of the position of a displaceable structure comprising: a position representing member mounted for movement in response to displacement of the structure; sensing elements spaced apart along the defined path such that each element is associated with a respective location along the defined path; means operatively coupling the elements into respective pairs of elements, having, for each pair of elements, an output producing a signal only when a single element of its respective pair is responding to the presence of the member; signal producing members each operative for producing a signal representing a predetermined logic state in response to a predetermined input signal, the number of the signal producing members being smaller than the number of the sensing elements; and circuit means operatively connecting the outputs to the signal producing members for causing a signal at each output to produce a predetermined input signal at a corresponding signal producing member and for causing a predetermined input signal to be produced at least one the signal producing member whenever a signal is present at either one of at least two of the outputs

  10. Photon beam position monitor

    Science.gov (United States)

    Kuzay, Tuncer M.; Shu, Deming

    1995-01-01

    A photon beam position monitor for use in the front end of a beamline of a high heat flux and high energy photon source such as a synchrotron radiation storage ring detects and measures the position and, when a pair of such monitors are used in tandem, the slope of a photon beam emanating from an insertion device such as a wiggler or an undulator inserted in the straight sections of the ring. The photon beam position monitor includes a plurality of spaced blades for precisely locating the photon beam, with each blade comprised of chemical vapor deposition (CVD) diamond with an outer metal coating of a photon sensitive metal such as tungsten, molybdenum, etc., which combination emits electrons when a high energy photon beam is incident upon the blade. Two such monitors are contemplated for use in the front end of the beamline, with the two monitors having vertically and horizontally offset detector blades to avoid blade "shadowing". Provision is made for aligning the detector blades with the photon beam and limiting detector blade temperature during operation.

  11. Beam Position Monitor Engineering

    International Nuclear Information System (INIS)

    Smith, Stephen R.

    1996-07-01

    The design of beam position monitors often involves challenging system design choices. Position transducers must be robust, accurate, and generate adequate position signal without unduly disturbing the beam. Electronics must be reliable and affordable, usually while meeting tough requirements on precision. accuracy, and dynamic range. These requirements may be difficult to achieve simultaneously, leading the designer into interesting opportunities for optimization or compromise. Some useful techniques and tools are shown. Both finite element analysis and analytic techniques will be used to investigate quasi-static aspects of electromagnetic fields such as the impedance of and the coupling of beam to striplines or buttons. Finite-element tools will be used to understand dynamic aspects of the electromagnetic fields of beams, such as wake-fields and transmission-line and cavity effects in vacuum-to-air feed through. Mathematical modeling of electrical signals through a processing chain will be demonstrated, in particular to illuminate areas where neither a pure time-domain nor a pure frequency-domain analysis is obviously advantageous. Emphasis will be on calculational techniques, in particular on using both time-domain and frequency domain approaches to the applicable parts of interesting problems

  12. Position reconstruction in LUX

    Science.gov (United States)

    Akerib, D. S.; Alsum, S.; Araújo, H. M.; Bai, X.; Bailey, A. J.; Balajthy, J.; Beltrame, P.; Bernard, E. P.; Bernstein, A.; Biesiadzinski, T. P.; Boulton, E. M.; Brás, P.; Byram, D.; Cahn, S. B.; Carmona-Benitez, M. C.; Chan, C.; Currie, A.; Cutter, J. E.; Davison, T. J. R.; Dobi, A.; Druszkiewicz, E.; Edwards, B. N.; Fallon, S. R.; Fan, A.; Fiorucci, S.; Gaitskell, R. J.; Genovesi, J.; Ghag, C.; Gilchriese, M. G. D.; Hall, C. R.; Hanhardt, M.; Haselschwardt, S. J.; Hertel, S. A.; Hogan, D. P.; Horn, M.; Huang, D. Q.; Ignarra, C. M.; Jacobsen, R. G.; Ji, W.; Kamdin, K.; Kazkaz, K.; Khaitan, D.; Knoche, R.; Larsen, N. A.; Lenardo, B. G.; Lesko, K. T.; Lindote, A.; Lopes, M. I.; Manalaysay, A.; Mannino, R. L.; Marzioni, M. F.; McKinsey, D. N.; Mei, D.-M.; Mock, J.; Moongweluwan, M.; Morad, J. A.; Murphy, A. St. J.; Nehrkorn, C.; Nelson, H. N.; Neves, F.; O'Sullivan, K.; Oliver-Mallory, K. C.; Palladino, K. J.; Pease, E. K.; Rhyne, C.; Shaw, S.; Shutt, T. A.; Silva, C.; Solmaz, M.; Solovov, V. N.; Sorensen, P.; Sumner, T. J.; Szydagis, M.; Taylor, D. J.; Taylor, W. C.; Tennyson, B. P.; Terman, P. A.; Tiedt, D. R.; To, W. H.; Tripathi, M.; Tvrznikova, L.; Uvarov, S.; Velan, V.; Verbus, J. R.; Webb, R. C.; White, J. T.; Whitis, T. J.; Witherell, M. S.; Wolfs, F. L. H.; Xu, J.; Yazdani, K.; Young, S. K.; Zhang, C.

    2018-02-01

    The (x, y) position reconstruction method used in the analysis of the complete exposure of the Large Underground Xenon (LUX) experiment is presented. The algorithm is based on a statistical test that makes use of an iterative method to recover the photomultiplier tube (PMT) light response directly from the calibration data. The light response functions make use of a two dimensional functional form to account for the photons reflected on the inner walls of the detector. To increase the resolution for small pulses, a photon counting technique was employed to describe the response of the PMTs. The reconstruction was assessed with calibration data including 83mKr (releasing a total energy of 41.5 keV) and 3H (β- with Q = 18.6 keV) decays, and a deuterium-deuterium (D-D) neutron beam (2.45 MeV) . Within the detector's fiducial volume, the reconstruction has achieved an (x, y) position uncertainty of σ = 0.82 cm and σ = 0.17 cm for events of only 200 and 4,000 detected electroluminescence photons respectively. Such signals are associated with electron recoils of energies ~0.25 keV and ~10 keV, respectively. The reconstructed position of the smallest events with a single electron emitted from the liquid surface (22 detected photons) has a horizontal (x, y) uncertainty of 2.13 cm.

  13. Early growth response gene-2 (Egr-2 regulates the development of B and T cells.

    Directory of Open Access Journals (Sweden)

    Suling Li

    2011-04-01

    Full Text Available Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the development of T, B and NKT cells in Egr-2 transgenic mice and the roles in the regulation of distinct stages of B and T cell development.The expression of Egr1, 2 and 3 were analysed at different stages of T and B cell development by RT-PCT and results showed that the expression was strictly regulated at different stages. Forced expression of Egr-2 in CD2(+ lymphocytes resulted in a severe reduction of CD4(+CD8(+ (DP cells in thymus and pro-B cells in bone marrow, which was associated with reduced expression of Notch1 in ISP thymocytes and Pax5 in pro-B cells, suggesting that retraction of Egr-2 at the ISP and pro-B cell stages is important for the activation of lineage differentiation programs. In contrast to reduction of DP and pro-B cells, Egr-2 enhanced the maturation of DP cells into single positive (SP T and NKT cells in thymus, and immature B cells into mature B cells in bone marrow.Our results demonstrate that Egr-2 expressed in restricted stages of lymphocyte development plays a dynamic, but similar role for the development of T, NKT and B cells.

  14. Bacterial Activity and Their Physiological Characteristics in the Sediments of O DP Holes 1202A and 1202D, Okinawa Trough, Western Pacific

    Directory of Open Access Journals (Sweden)

    Jiin-Shuh Jean

    2005-01-01

    Full Text Available Bacterial strains were isolated and identified from the down-core sediments of Site 1202 of ODP Leg 195 in the Okinawa Trough. Their phylogenetic relationships and physiological characteristics were determined. The isolates were cultured in aerobic and anaerobic sulfate-reducing and fermentative media at temperatures of _ and _ The results showed that there were gram-positive/negative rod- and/or sphere-shaped bacteria in the sediments at all depths from 3 to 358.3 meters below the seafloor (mbsf, but no bacteria were present at depths greater than 358.3 mbsf (> 64.73 ka in age of sediment were isolated (maximum core depth 406.5 mbsf. On the basis of the nucleotide similarities of 16S rDNA and reconstructed phylogeny, the bacterial isolates of the sediments of 10.1 mbsf (1202A002H and 241.2 mbsf (1202D027X were shown to share high identities with the Bacillus subtilis and Pseudomonas putida or _ MPD-98 strains. Physiological experiments showed that the optimal temperature for growth of the studied bacteria was _ but the bacteria obtained at some depths could tolerate temperatures up to _ The maximum salinity allowed for the growth of the cultured bacteria was 60 _ 650/00. The optimal pH for bacterial growth was 7.0 - 8.3. None of the bacteria extracted from the studied sediments could survive at pH _ or _ These halotolerant bacteria were capable of making consumption of Fe, Cu2+ , Na+, K+, Mg2+ , Ca2+, and F−, but no biogenic minerals could be identified in the present study.

  15. Positively deflected anomaly mediation

    International Nuclear Information System (INIS)

    Okada, Nobuchika

    2002-01-01

    We generalize the so-called 'deflected anomaly mediation' scenario to the case where threshold corrections of heavy messengers to the sparticle squared masses are positive. A concrete model realizing this scenario is also presented. The tachyonic slepton problem can be fixed with only a pair of messengers. The resultant sparticle mass spectrum is quite different from that in the conventional deflected anomaly mediation scenario, but is similar to the one in the gauge mediation scenario. The lightest sparticle is mostly B-ino

  16. Dissimilar Impact Welding of 6111-T4, 5052-H32 Aluminum Alloys to 22MnB5, DP980 Steels and the Structure-Property Relationship of a Strongly Bonded Interface

    Science.gov (United States)

    Liu, Bert; Vivek, Anupam; Presley, Michael; Daehn, Glenn S.

    2018-03-01

    The ability to weld high-strength aluminum to high-strength steel is highly desired for vehicle lightweighting but difficult to attain by conventional means. In this work, vaporizing foil actuator welding was used to successfully weld four Al/Fe combinations consisting of high-strength alloys: AA5052-H32, AA6111-T4, DP980, and 22MnB5. Flyer velocities up to 727 m/s were reached using 10 kJ input energy. In lap-shear testing, samples primarily failed in base aluminum near the aluminum's native strength, showing that the welds were stronger than a base metal and that the base metal was not significantly weakened by the welding process. A particularly strong weld area was studied by transmission electron microscopy to shed light on the microstructural features of strong impact welds. It was found to be characterized by a continuously bonded, fully crystalline interface, extremely fine (nanoscale) grains, mesoscopic as well as microscopic wavy features, and lack of large continuous intermetallic compounds.

  17. Triangulation positioning system network

    Directory of Open Access Journals (Sweden)

    Sfendourakis Marios

    2017-01-01

    Full Text Available This paper presents ongoing work on localization and positioning through triangulation procedure for a Fixed Sensors Network - FSN.The FSN has to work as a system.As the triangulation problem becomes high complicated in a case with large numbers of sensors and transmitters, an adequate grid topology is needed in order to tackle the detection complexity.For that reason a Network grid topology is presented and areas that are problematic and need further analysis are analyzed.The Network System in order to deal with problems of saturation and False Triangulations - FTRNs will have to find adequate methods in every sub-area of the Area Of Interest - AOI.Also, concepts like Sensor blindness and overall Network blindness, are presented. All these concepts affect the Network detection rate and its performance and ought to be considered in a way that the network overall performance won’t be degraded.Network performance should be monitored contentiously, with right algorithms and methods.It is also shown that as the number of TRNs and FTRNs is increased Detection Complexity - DC is increased.It is hoped that with further research all the characteristics of a triangulation system network for positioning will be gained and the system will be able to perform autonomously with a high detection rate.

  18. Vegetarian-style dietary pattern during adolescence has long-term positive impact on bone from adolescence to young adulthood: a longitudinal study.

    Science.gov (United States)

    Movassagh, Elham Z; Baxter-Jones, Adam D G; Kontulainen, Saija; Whiting, Susan; Szafron, Michael; Vatanparast, Hassan

    2018-02-28

    The amount of bone accrued during adolescence is an important determinant of later osteoporosis risk. Little is known about the influence of dietary patterns (DPs) on the bone during adolescence and their potential long-term implications into adulthood. We examined the role of adolescent DPs on adolescent and young adult bone and change in DPs from adolescence to young adulthood. We recruited participants from the Saskatchewan Pediatric Bone Mineral Accrual Study (1991-2011). Data from 125 participants (53 females) for adolescent analysis (age 12.7 ± 2 years) and 115 participants (51 females) for adult analysis (age 28.2 ± 3 years) were included. Bone mineral content (BMC) and areal bone mineral density (aBMD) of total body (TB), femoral neck (FN) and lumbar spine (LS) were measured using dual-energy X-ray absorptiometry. Adolescent dietary intake data from multiple 24-h recalls were summarized into 25 food group intakes and were used in the principal component analysis to derive DPs during adolescence. Associations between adolescent DPs and adolescent or adult BMC/BMD were analyzed using multiple linear regression and multivariate analysis of covariance while adjusting for sex, age, the age of peak height velocity, height, weight, physical activity and total energy intake. Generalized estimating equations were used for tracking DPs. We derived five DPs including "Vegetarian-style", "Western-like", "High-fat, high-protein", "Mixed" and "Snack" DPs. The "Vegetarian-style" DP was a positive independent predictor of adolescent TBBMC, and adult TBBMC, TBaBMD (P adolescent TBaBMD and young adult TBBMC, TBaBMD, FNBMC and FNaBMD were 5%, 8.5%, 6%, 10.6% and 9% higher, respectively, in third quartile of "Vegetarian-style" DP compared to first quartile (P adolescence to adulthood. There were an upward trend in adherence to "Vegetarian-style" DP and an downward trend in adherence to "High-fat, high-protein" DP from adolescence to young adulthood (P

  19. Positive void reactivity

    International Nuclear Information System (INIS)

    Diamond, D.J.

    1992-09-01

    This report is a review of some of the important aspects of the analysis of large loss-of-coolant accidents (LOCAs). One important aspect is the calculation of positive void reactivity. To study this subject the lattice physics codes used for void worth calculations and the coupled neutronic and thermal-hydraulic codes used for the transient analysis are reviewed. Also reviewed are the measurements used to help validate the codes. The application of these codes to large LOCAs is studied with attention focused on the uncertainty factor for the void worth used to bias the results. Another aspect of the subject dealt with in the report is the acceptance criteria that are applied. This includes the criterion for peak fuel enthalpy and the question of whether prompt criticality should also be a criterion. To study the former, fuel behavior measurements and calculations are reviewed. (Author) (49 refs., 2 figs., tab.)

  20. Conference - La discipline positive

    CERN Multimedia

    Staff Association

    2018-01-01

    Eduquer avec fermeté et bienveillance Véronique Genevay Jeudi 31 mai à 19h00 CERN Meyrin, Salle du Conseil 503-1-001 Venez vous familiariser avec la pensée et le positionnement développé dans la discipline positive. Une approche ni permissive, ni punitive, qui vise à enseigner aux enfants des compétences comme la confiance en soi, l’autonomie, le respect de soi-même et le respect mutuel, la responsabilité, la coopération… Inscrivez-vous : https://indico.cern.ch/e/disciplinepositive   Pour plus d’informations : staff.association@cern.ch ou (+41) 022 766 37 38

  1. Position paper on mesotherapy.

    Science.gov (United States)

    Sarkar, Rashmi; Garg, Vijay Kumar; Mysore, Venkataram

    2011-01-01

    Mesotherapy is a controversial cosmetic procedure which has received publicity among the lay people, in the internet and in the media. It refers to minimally invasive techniques which consist of the use of intra- or subcutaneous injections containing liquid mixture of compounds (pharmaceutical and homeopathic medications, plant extracts, vitamins and other ingredients) to treat local medical and cosmetic conditions. This position paper has examined the available evidence and finds that acceptable scientific evidence for its effectiveness and safety is lacking. IADVL taskforce, therefore would like to state that the use of this technique remains controversial at present. Further research and well-designed controlled scientific studies are required to substantiate the claims of benefit of this mode of therapy.

  2. Position paper on mesotherapy

    Directory of Open Access Journals (Sweden)

    Rashmi Sarkar

    2011-01-01

    Full Text Available Mesotherapy is a controversial cosmetic procedure which has received publicity among the lay people, in the internet and in the media. It refers to minimally invasive techniques which consist of the use of intra- or subcutaneous injections containing liquid mixture of compounds (pharmaceutical and homeopathic medications, plant extracts, vitamins and other ingredients to treat local medical and cosmetic conditions. This position paper has examined the available evidence and finds that acceptable scientific evidence for its effectiveness and safety is lacking. IADVL taskforce, therefore would like to state that the use of this technique remains controversial at present. Further research and well-designed controlled scientific studies are required to substantiate the claims of benefit of this mode of therapy.

  3. Galileo positioning technology

    CERN Document Server

    Lohan, Elena; Sand, Stephan; Hurskainen, Heikki

    2015-01-01

    This book covers multi-band Galileo receivers (especially E1-E5 bands of Galileo) and addresses all receiver building blocks, from the antenna and front end, through details of the baseband receiver processing blocks, up to the navigation processing, including the Galileo message structure and Position, Velocity, Time (PVT) computation. Moreover, hybridization solutions with communications systems for improved localization are discussed and an open-source GNSS receiver platform (available for download) developed at Tampere University of Technology (TUT) is addressed in detail. • Takes a holistic approach to GALILEO and related systems, such as EGNOS and hybrid solutions on mobile phones; • Provides an invaluable reference to Binary Offset Carrier modulations and related families, which are some of the trademarks of GALILEO; • Includes a detailed survey of GALILEO receiver research in Europe and existing software-defined radio (SDR) GALILEO receiver implementations; • Addresses the multiple challen...

  4. ISSP Position Stand

    DEFF Research Database (Denmark)

    Ryba, Tatiana; Stambulova, Natalia; Si, Gangyan

    2013-01-01

    The multicultural landscape of contemporary sport sets a challenge to rethink sport and exercise psychology research and practice through a culturally re␣exive lens. This ISSP Position Stand provides a rigorous synthesis and engagement with existing scholarship to outline a roadmap for future work...... in the ␣eld. The shift to culturally competent sport and exercise psychology implies: (a) recognizing hidden ethnocentric philosophical assumptions permeating much of the current theory, research, and practice; (b) transitioning to professional ethics in which difference is seen as not inherent and ␣xed...... but as relational and ␣uid; and (c) focusing on meaning (instead of cause) in cross-cultural and cultural research projects, and cultural praxis work. In the paper, we ␣rst provide an overview of the concepts of cultural competence and ethics of difference. Second, we present a step-by-step approach for developing...

  5. The Power of Positive Emotions

    Science.gov (United States)

    ... Safe Videos for Educators Search English Español The Power of Positive Emotions KidsHealth / For Teens / The Power ... great one. 2. Practice Positivity Every Day Building habits that encourage us to feel more positive emotions ...

  6. Detecting positive quadrant dependence and positive function dependence

    NARCIS (Netherlands)

    Janic-Wróblewska, A.; Kallenberg, W.C.M.; Ledwina, T.

    2004-01-01

    There is a lot of interest in positive dependence going beyond linear correlation. In this paper three new rank tests for testing independence against positive dependence are introduced. The first one is directed on positive quadrant dependence, the second and third one concentrate on positive

  7. Detecting positive quadrant dependence and positive function dependence

    NARCIS (Netherlands)

    Janic-Wróblewska, A.; Kallenberg, W.C.M.; Ledwina, T.

    2003-01-01

    There is a lot of interest in positive dependence going beyond linear correlation. In this paper three new rank tests for testing independence against positive dependence are introduced. The first one is directed on positive quadrant dependence, the second and third one concentrate on positive

  8. Relationship of condylar position to disc position and morphology

    Energy Technology Data Exchange (ETDEWEB)

    Incesu, L.; Taskaya-Yilmaz, N. E-mail: nergizy@omu.edu.tr; Oeguetcen-Toller, M.; Uzun, E

    2004-09-01

    Introduction/objective: The purpose of this study was to assess whether condylar position, as depicted by magnetic resonance imaging, was an indicator of disc morphology and position. Methods and material: One hundred and twenty two TMJs of 61 patients with temporomandibular joint disorder were examined. Condylar position, disc deformity and degree of anterior disc displacement were evaluated by using magnetic resonance imaging. Results and discussion: Posterior condyle position was found to be the main feature of temporomandibular joints with slight and moderate anterior disc displacement. No statistical significance was found between the condylar position, and reducing and nonreducing disc positions. On the other hand, superior disc position was found to be statistically significant for centric condylar position. Conclusion: It was concluded that posterior condyle position could indicate anterior disc displacement whereas there was no relation between the position of condyle and the disc deformity.

  9. Preferential effects of leptin on CD4 T cells in central and peripheral immune system are critically linked to the expression of leptin receptor

    Energy Technology Data Exchange (ETDEWEB)

    Kim, So Yong; Lim, Ju Hyun [Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746 (Korea, Republic of); Choi, Sung Won [Department of Molecular Biology, School of Arts and Sciences (S.W.C), Cornell University, Ithaca, NY 18450 (United States); Kim, Miyoung; Kim, Seong-Tae [Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746 (Korea, Republic of); Kim, Min-Seon; Cho, You Sook [Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-600 (Korea, Republic of); Chun, Eunyoung, E-mail: chun.eunyoung@gmail.com [Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746 (Korea, Republic of); Lee, Ki-Young, E-mail: thylee@med.skku.ac.kr [Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746 (Korea, Republic of)

    2010-04-09

    Leptin can enhance thymopoiesis and modulate the T-cell immune response. However, it remains controversial whether these effects correlate with the expression of leptin receptor, ObR. We herein addressed this issue by using in vivo animal models and in vitro culture systems. Leptin treatment in both ob/ob mice and normal young mice induced increases of CD4 SP thymocytes in thymus and CD4 T cells in the periphery. Interestingly, expression of the long form ObR was significantly restricted to DN, DP and CD4 SP, but not CD8 SP thymocytes. Moreover, in the reaggregated DP thymocyte cultures with leptin plus TSCs, leptin profoundly induced differentiation of CD4 SP but not CD8 SP thymocytes, suggesting that the effects of leptin on thymocyte differentiation might be closely related to the expression of leptin receptor in developing thymocytes. Surprisingly, ObR expression was markedly higher in peripheral CD4 T cells than that in CD8 T cells. Furthermore, leptin treatment with or without IL-2 and PHA had preferential effects on cell proliferation of CD4 T cells compared to that of CD8 T cells. Collectively, these data provide evidence that the effects of leptin on differentiation and proliferation of CD4 T cells might be closely related to the expression of leptin receptor.

  10. Preferential effects of leptin on CD4 T cells in central and peripheral immune system are critically linked to the expression of leptin receptor

    International Nuclear Information System (INIS)

    Kim, So Yong; Lim, Ju Hyun; Choi, Sung Won; Kim, Miyoung; Kim, Seong-Tae; Kim, Min-Seon; Cho, You Sook; Chun, Eunyoung; Lee, Ki-Young

    2010-01-01

    Leptin can enhance thymopoiesis and modulate the T-cell immune response. However, it remains controversial whether these effects correlate with the expression of leptin receptor, ObR. We herein addressed this issue by using in vivo animal models and in vitro culture systems. Leptin treatment in both ob/ob mice and normal young mice induced increases of CD4 SP thymocytes in thymus and CD4 T cells in the periphery. Interestingly, expression of the long form ObR was significantly restricted to DN, DP and CD4 SP, but not CD8 SP thymocytes. Moreover, in the reaggregated DP thymocyte cultures with leptin plus TSCs, leptin profoundly induced differentiation of CD4 SP but not CD8 SP thymocytes, suggesting that the effects of leptin on thymocyte differentiation might be closely related to the expression of leptin receptor in developing thymocytes. Surprisingly, ObR expression was markedly higher in peripheral CD4 T cells than that in CD8 T cells. Furthermore, leptin treatment with or without IL-2 and PHA had preferential effects on cell proliferation of CD4 T cells compared to that of CD8 T cells. Collectively, these data provide evidence that the effects of leptin on differentiation and proliferation of CD4 T cells might be closely related to the expression of leptin receptor.

  11. SQUID position sensor development

    Science.gov (United States)

    Torii, Rodney

    1996-11-01

    I describe the development of an inductance position sensor for the STEP (satellite test of the equivalence principle) accelerometer. I have measured the inductance (with an experimental error of 0.5%) of a single-turn thin-film niobium pick-up coil as a function of the distance from a thin-film niobium disc (both at 4.2 K and superconducting). The circular pick-up coil had a diameter of 4 cm with a track width of 0264-9381/13/11A/022/img1. The disc (mock test mass) had a diameter of 4 cm. The distance range between the coil and disc was set by the range of a low-temperature differential capacitance sensor: 0 - 2 mm with a resolution of 0264-9381/13/11A/022/img2. The full range of the low-temperature translation stage was 0 - 4 mm. The inductance was measured using an LCR meter in a four-wire configuration. The measured inductance was compared to the inductance of a circular loop above a superconducting plane. Due to the fact that the thin-film disc is of finite size, the calculation differed from experiment by as much as 12%. I have also calculated the inductance by segmenting the thin-film niobium disc into 500 concentric rings (each with a width of 0264-9381/13/11A/022/img3). A discrepancy between calculation and experiment of approximately 3% was found.

  12. Gathering positive experience

    CERN Document Server

    2009-01-01

    Last Monday, the new CERN Machine Advisory Committee (CMAC) met for the first time, and we had good news to tell its members. Over the weekend, injection tests for both LHC beams were successfully carried out. In other words, we’ve had beam in the LHC for the first time since September 2008. That’s a good feeling, but it’s no reason for complacency. There’s still a long way to go before first physics at the new energy frontier. As the Bulletin has reported over recent weeks, we’re gathering a lot of positive experience with the new quench detection and protection system (QPS), which is already allowing us to monitor the LHC far better than we were able to in the past. So far, the QPS for three of the LHC’s eight sectors has been put through its paces, and we’ve also power tested those sectors to 2000 amperes, the equivalent of around 1.2 TeV per beam. The next step is to slowly increase the current to 4000 amperes, and...

  13. Climate change policy position

    International Nuclear Information System (INIS)

    2002-11-01

    The Canadian Association of Petroleum Producers (CAPP) is a firm believer in the need to take action to mitigate the risks associated with climate change, and that clear government policy is called for. The principles of sustainable development must guide this policy development effort. The initiatives required to address greenhouse gas emissions over both the short and long term must be carefully considered, and it is up to industries to ensure their production efficiency and emission intensity. Promoting improved performance of industries in Canada and developing technology that can be deployed internationally for larger global effects represents Canada's best contribution to progress on greenhouse gas emissions. The increase in energy demand along with increases in population and economic growth have contributed to an increase in greenhouse gas emissions despite improved energy efficiency in industry. Significant damage to the economy will result if Canada is to meet its commitment under the Kyoto Protocol, forcing the country to buy large quantities of foreign credits instead of using those funds for increased research and development. CAPP indicated that an effective plan must be: balanced, equitable, responsible, competitive, focused on technology and innovation, and based on agreements on sectoral plans. Each of these principles were discussed, followed by the fundamentals of approach for upstream oil and gas. The framework for climate change policy was described as well as the elements of a sector plan. CAPP wants to work with all levels of government on an appropriate plan for Canada, that considers our unique circumstances. Canada can play a significant role on the international stage by properly implementing the policy position proposed by the CAPP without unnecessary risks to the economy. refs

  14. Dynamic Positioning Capability Analysis for Marine Vessels Based on A DPCap Polar Plot Program

    Science.gov (United States)

    Wang, Lei; Yang, Jian-min; Xu, Sheng-wen

    2018-03-01

    Dynamic positioning capability (DPCap) analysis is essential in the selection of thrusters, in their configuration, and during preliminary investigation of the positioning ability of a newly designed vessel dynamic positioning system. DPCap analysis can help determine the maximum environmental forces, in which the DP system can counteract in given headings. The accuracy of the DPCap analysis is determined by the precise estimation of the environmental forces as well as the effectiveness of the thrust allocation logic. This paper is dedicated to developing an effective and efficient software program for the DPCap analysis for marine vessels. Estimation of the environmental forces can be obtained by model tests, hydrodynamic computation and empirical formulas. A quadratic programming method is adopted to allocate the total thrust on every thruster of the vessel. A detailed description of the thrust allocation logic of the software program is given. The effectiveness of the new program DPCap Polar Plot (DPCPP) was validated by a DPCap analysis for a supply vessel. The present study indicates that the developed program can be used in the DPCap analysis for marine vessels. Moreover, DPCap analysis considering the thruster failure mode might give guidance to the designers of vessels whose thrusters need to be safer.

  15. Exposing Position Uncertainty in Middleware

    DEFF Research Database (Denmark)

    Langdal, Jakob; Kjærgaard, Mikkel Baun; Toftkjær, Thomas

    2010-01-01

    Traditionally, the goal for positioning middleware is to provide developers with seamless position transparency, i.e., providing a connection between the application domain and the positioning sensors while hiding the complexity of the positioning technologies in use. A key part of the hidden com...

  16. Positive Technologies for Understanding and Promoting Positive Emotions.

    Science.gov (United States)

    Baños, Rosa María; Carrillo, Alba; Etchemendy, Ernestina; Botella, Cristina

    2017-10-26

    Information and Communication Technologies (ICTs) have become increasingly present in our lives, and their use has spread considerably. This paper presents a review of the way ICTs can help practitioners and researchers to study, promote, and train positive emotions. It is framed within the field of Positive Technologies: the applied scientific approach to the study of the use of technology to improve the quality of personal experience, with the goal of increasing wellbeing. First, the article presents an introduction to the topic of technologies and positive emotions. Then, it describes how ICTs can aid in monitoring, assessing, promoting, modifying, and training positive emotions. Finally, implications and future directions of the role of Positive Technologies in positive emotions are discussed. The authors conclude that, in the near future, Positive Technologies and the field of positive emotions will interact synergistically, producing an exponential growth in the understanding and promotion of positive emotions.

  17. Apparent genetic difference between hypothyroid patients with blocking-type thyrotropin receptor antibody and those without, as shown by restriction fragement length polymorphism analyses of HLA-DP loci

    Energy Technology Data Exchange (ETDEWEB)

    Inoue, Daisuke; Sugawa, Hideo; Akamizu, Takashi; Mori, Toru (Kyoto Univ. School of Medicine, Kyoto (Japan)); Sato, Kaoru; Inoko, Hidetoshi; Tsuji, Kimiyoshi (Tokai Univ. School of Medicine, Kanagawa (Japan)); Maeda, Masahiro (Nichirei Corp., Tokyo (Japan))

    1993-09-01

    HLA types in Japanese patients with primary hypothyroidism were analyzed to see whether those with blocking-type TSH receptor antibody (TSH-R BAb M) differed genetically from those with idiopathic myxedema (IM). HLA typings of -A, -B, -C, -DR, and -DQ (73 antigens) were performed serologically, and those of -D and -DP (29 antigens) were analyzed by the restriction fragment length polymorphism method. Thirty patients were studied with TSH-R BAb M, and 28 with IM. The data were analyzed and compared with previous results from 88 Graves' patients, 46 Hashimoto patients, and 186 control subjects. Overall, 192 patients with 4 autoimmune thyroid disorders showed a decrease in -Aw19 and an increase in -DQw4 (corrected P < 0.05) and significant associations of -Aw33, -Bw46, -Cw3, -DRw8, -DR9, and -DQw3. In TSH-R BAb M patients, increases in -B35, -Bw60, and -Dw8 and decreases in -DR4 and -DPw2 were seen, whereas IM patients showed increased -DPw2, -Bw61, and -Dw23. In comparisons between TSH-R-BAb M and IM, the difference in -DPw2 was highly significant. HLA-B35 differed significantly in these 2 types of hypothyroidism. In conclusion, TSH-R BAb M patients have decreased frequency of -DPw2 and are genetically similar to Graves' disease, whereas IM patients are characterized by high frequency of -DPw2 and are genetically similar to Hashimoto's thyroiditis. 39 refs., 2 figs., 3 tabs.

  18. Secure positioning in wireless networks

    DEFF Research Database (Denmark)

    Capkun, Srdjan; Hubaux, Jean-Pierre

    2006-01-01

    So far, the problem of positioning in wireless networks has been studied mainly in a non-adversarial settings. In this work, we analyze the resistance of positioning techniques to position and distance spoofing attacks. We propose a mechanism for secure positioning of wireless devices, that we call...... Verifiable Multilateration. We then show how this mechanism can be used to secure positioning in sensor networks. We analyze our system through simulations....

  19. RES MARCH COV 07 PRINT DP

    Indian Academy of Sciences (India)

    Stem cells constitute an important class of cells in the body that have the ability to ... These cells are pluripotent, that is, they have the potential to give rise to ..... when a population of stem cells is injected into the human body – will it remain the ...

  20. RES MARCH COV 07 PRINT DP

    Indian Academy of Sciences (India)

    motors on AC. As a result of the “War of Currents”, Edison and. Westinghouse went nearly bankrupt. So in 1897, Tesla released. Westinghouse from contract obligations, providing Westinghouse a break from Tesla's patent royalties. In doing so Tesla gave away nearly 12 million dollars in royalty that he would have received.