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Sample records for politsei lesanded hes

  1. Politsei pelgab oma majas vargaid

    Index Scriptorium Estoniae

    2008-01-01

    Ilmunud ka: Severnoje Poberezhje 17. jaan., lk. 3. President Toomas Hendrik Ilves külastas Jõhvi politseiosakonda. Presidendis ja tema kaaskonnas tekitas imestust, et politsei üldkasutatav printer oli peidetud lukustatud metallvõre taha. President avaldas lootust, et praegused viletsad töötingimused asenduvad peagi 21. sajandi väärilistega

  2. Eesti piloodid Afganistanis täitsid ülesande

    Index Scriptorium Estoniae

    2012-01-01

    Afganistanis teenivad Eesti õhuväe kopteripiloodid kapten Rene Kallis ja kapten Martin Noorsalu täitsid oma esimese teenistusülesande, lennates UH-60 Black Hawk tüüpi kopteritega. Ülesanne oli meedikute transport Camp Bastioni välihospidali. Pärast tutvumislende hakkavad piloodid lendama meditsiinilise evakuatsiooni üksuse koosseisus

  3. Tumedanahaline Eesti kodanik politsei silmis kahtlane element / Eve Heinla

    Index Scriptorium Estoniae

    Heinla, Eve, 1966-

    2005-01-01

    Politsei ei lubanud Briti peaministri visiidi ajal tumedanahalist pakistanlasest Eesti kodanikku Tahir Husseini Raekoja platsile. Vt. samas: Kadri Paas. Suurbritannia peaministrit sõidutas Tallinnas soomlane

  4. Saksa politsei peab eestlast luksusautode varaste juhiks / Rasmus Kagge

    Index Scriptorium Estoniae

    Kagge, Rasmus, 1977-

    2006-01-01

    Ilmunud ka: Postimees : na russkom jazõke 14. märts lk. 5. Saksa politsei on vahistanud eestlasest ärimehe Ted Hildebrandi, keda arvatakse olevat ligi 100 miljoni krooni eest luksusautosid varastanud grupeeringu juht

  5. Politsei ostab ja rendib 159 uut teenistusautot / Kadri Põldaru

    Index Scriptorium Estoniae

    Põldaru, Kadri

    2005-01-01

    Politseiamet sõlmib lepingu firmadega Amserv Auto ja Elke Auto, ostes nendelt ühispakkumise alusel 59 Toyota Corollat. 100 Nissan Primera kasutusrendiks sõlmib politsei lepingu AS-iga Balti Liising

  6. Politsei hakkab kiiruskaamera abil kihutajaid trahvima / Ingvar Bärenklau

    Index Scriptorium Estoniae

    Bärenklau, Ingvar, 1967-

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke 13. apr. lk. 5. Politsei alustab Eesti teedel teste eravärvides politseiautole paigutatud mobiilse kiiruskaameraga, mille abil saab tuvastada kiiruseületajaid ja neid trahvida

  7. Politsei karistas "Kuldsõduri" autorit suulise hoiatusega / Dannar Leitmaa

    Index Scriptorium Estoniae

    Leitmaa, Dannar, 1982-

    2009-01-01

    Tallinnas Tõnismäe haljasalale "Kuldsõduri" püstitamise asjus politsei algatatud uurimismenetlus lõppes ja kunstnik Kristina Norman saab kuju tagasi. Kuldne pronkssõduri koopia on osa Eestit 53. Veneetsia biennaalil esindavast kunstiprojektist "After-War", mille valis riiki esindama rahvusvaheline žürii

  8. Londoni pommipanijate jahil tappis politsei kahtlase mehe / Kaivo Kopli

    Index Scriptorium Estoniae

    Kopli, Kaivo

    2005-01-01

    Briti politsei tulistas pommipanijaid jahtides Londoni Stocwelli metroojaamas surnuks kahtlase mehe. Vastutuse 7. juulil ja 21. juulil Londonis toimunud plahvatuste organiseerimises on võtnud endale al-Qaida terrorivõrguga seotud rühmitus Abu Hafs al-Masri brigaad. Lisa: Briti muslimid tunnevad muret

  9. Politsei areng läbi aegade / Priit Suve

    Index Scriptorium Estoniae

    Suve, Priit, 1973-

    2011-01-01

    Artiklis vaadeldakse politseisüsteemi ajaloo lõikes, muutusi suhtumises haridusse, demokraatlikesse ja moraalsetesse väärtustesse. Jälgitakse tsiviil- ja militaarorganisatsiooni võimalikke mõjusid ning legitiimsust kui politseitöö alust

  10. Politseis ja piirivalves võib kaduda kuni 360 töökohta / Urmas Seaver

    Index Scriptorium Estoniae

    Seaver, Urmas, 1973-

    2009-01-01

    Seoses eelarvekärpe ja 1. jaanuarist tööle hakkava politsei-, piiirvalve- ja kodakondsus- ja migratsioniametit liitva uue ühendasutuse loomisega võib kaduda politseis ja piirivalves 350-360 töökohta

  11. Tagasivaated Eesti politsei lähiaastatele / Margus Mikomägi

    Index Scriptorium Estoniae

    Mikomägi, Margus, 1956-

    2001-01-01

    Järgneb: Nädaline, 22. sept. 2001, lk. 8; 29. sept. 2001, lk. 8; 6. okt. 2001, lk. 12; 13. okt. 2001, lk. 8; 20. okt. 2001, lk. 12. See mis täna politseis ja politsei ümber sünnib, on otseselt seotud lähiminevikus tehtud vigadega

  12. Eesti politsei kogeb seninägematuid IT-uuendusi / Mart Raudsaar

    Index Scriptorium Estoniae

    Raudsaar, Mart, 1973-

    2007-01-01

    Eesti politseis 2007. aastal juurutatavatest tarkvaraprojektidest. Keskseid märksõnu on uue operatiiv-raadiosidesüsteemi (ORS) käivitamine, mis võimaldab politsei, piirivalve ja päästeameti ühtset suhtlust. Vt. samas: Merle Küngas. Uus operatiivraadiosidevõrk tähendab suuremat turvalisust

  13. Eesti politsei hävitamine esimesel Nõukogude okupatsiooniaastal 1940/1941 / Mai Krikk

    Index Scriptorium Estoniae

    Krikk, Mai, 1946-

    2008-01-01

    Ülevaade Eesti politsei hävitamisest politseiametnike saatuse kaudu. Politseijuhid 1918-1940, lk. 145-148. - Lisad: 14. juunil 1941 represseeritud politseinikud ; represseeritud politseiametnikud 1940/1941

  14. Hispaania politsei otsib taga äärmusislamistide lõhkeainet / Pablo Veyrat

    Index Scriptorium Estoniae

    Veyrat, Pablo

    2008-01-01

    Ilmunud ka: Postimees : na russkom jazõke 29. jaan. 2008, lk. 6. Hispaania politsei otsib idarannikult pommimaterjali, mida kümme hiljuti vangistatud pakistanlast väidetavalt Barcelona metroos suitsiidirünnaku korraldamiseks kasutada kavatsesid. Lisa: Paljastatud vandenõu

  15. Eesti politsei saab uued sõrmejälgede võtmise aparaadid / Olger Nõmm

    Index Scriptorium Estoniae

    Nõmm, Olger

    2005-01-01

    PHARE programmi kaasabil teostuvast AFIS-i (Automated Fingerprint Identification System) projektist, mille raames Eesti Politsei ja Piirivalveamet saavad kaasaegsed sõrmejälgede võtmise seadmed. Hangitavate seadmete tüübid. Vt. samas: Projektist on kasu kolmele ametkonnale

  16. Türgi politsei süüdistab kurdidest inimõiguslasi / Vahur Koorits

    Index Scriptorium Estoniae

    Koorits, Vahur, 1981-

    2004-01-01

    Türgi politsei ja võimud süüdistavad vanglast vabanenud kurdi parlamendisaadikuid separatistlikes kihutuskõnedes ning kurdi keele rääkimises. Üks süüdistatu Leyla Zana on saanud 1995. aastal Andrei Zahharovi nimelise inimõiguste preemia

  17. SAP aitas Politsei- ja Piirivalveameti finantstöötajate arvu vähendada ligi kolm korda

    Index Scriptorium Estoniae

    2010-01-01

    Politsei- ja Piirivalveamet võttis kasutusele SAP tarkvara, mis on võimeline muutma asutuse finantsjuhtimise, personali-, palgaarvestuse ja logistika valdkondade tööprotsessid efektiivsemaks ja automatiseeritumaks

  18. [Miks uurib politsei Ottise ja ehitusfirma Facio vahelist tüli?] / Tarmo Vahter

    Index Scriptorium Estoniae

    Vahter, Tarmo, 1970-

    2006-01-01

    Seoses koolitüdruku Veronika Dari tapmisega, kus tegelikuks sihtmärgiks oli Riigi Kinnisvara juht Tiit Ottis, tunneb politsei huvi kolme riigi poolt tellitud ehitustöö vastu. Need on keskkriminaalpolitsei peakorteri ning Tallinna Pae ja Mustamäe gümnaasiumi renoveerimine. Uurimise käigust. Vt. samas: Katkeid Riigi Kinnisvara ja Facio Ehituse juhtide kirjavahetusest Pae ja Mustamäe gümnaasiumide remondi teemal. Tiit Ottis: "Mehed, te rikastute alusetult!"

  19. HES6 enhances the motility of alveolar rhabdomyosarcoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Wickramasinghe, Caroline M [MRC Cancer Cell Unit, Hutchison-MRC Research centre, Addenbrooke' s Hospital Cambridge, CB2 0XZ (United Kingdom); MRC Laboratory of Molecular Biology, Addenbrooke' s Hospital Cambridge, CB2 0QH (United Kingdom); Domaschenz, Renae [MRC Cancer Cell Unit, Hutchison-MRC Research centre, Addenbrooke' s Hospital Cambridge, CB2 0XZ (United Kingdom); Gene Regulation and Chromatin Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Campus, Du Cane Road, London W12 ONN (United Kingdom); Amagase, Yoko [MRC Cancer Cell Unit, Hutchison-MRC Research centre, Addenbrooke' s Hospital Cambridge, CB2 0XZ (United Kingdom); Department of Pathophysiology, Faculty of Pharmaceutical Sciences, Doshisha Women' s College of Liberal Arts, Kodo, Kyotanabe, Kyoto 610-0395 (Japan); Williamson, Daniel [Molecular Cytogenetics, The Institute of Cancer Research, Sutton SM2 5NG (United Kingdom); Northern Institute for Cancer Research, Paul O' Gorman Building, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH (United Kingdom); Missiaglia, Edoardo; Shipley, Janet [Molecular Cytogenetics, The Institute of Cancer Research, Sutton SM2 5NG (United Kingdom); Murai, Kasumi [MRC Cancer Cell Unit, Hutchison-MRC Research centre, Addenbrooke' s Hospital Cambridge, CB2 0XZ (United Kingdom); Jones, Philip H, E-mail: phj20@cam.ac.uk [MRC Cancer Cell Unit, Hutchison-MRC Research centre, Addenbrooke' s Hospital Cambridge, CB2 0XZ (United Kingdom)

    2013-01-01

    Absract: HES6, a member of the hairy-enhancer-of-split family of transcription factors, plays multiple roles in myogenesis. It is a direct target of the myogenic transcription factor MyoD and has been shown to regulate the formation of the myotome in development, myoblast cell cycle exit and the organization of the actin cytoskeleton during terminal differentiation. Here we investigate the expression and function of HES6 in rhabdomyosarcoma, a soft tissue tumor which expresses myogenic genes but fails to differentiate into muscle. We show that HES6 is expressed at high levels in the subset of alveolar rhabdomyosarcomas expressing PAX/FOXO1 fusion genes (ARMSp). Knockdown of HES6 mRNA in the ARMSp cell line RH30 reduces proliferation and cell motility. This phenotype is rescued by expression of mouse Hes6 which is insensitive to HES6 siRNA. Furthermore, expression microarray analysis indicates that the HES6 knockdown is associated with a decrease in the levels of Transgelin, (TAGLN), a regulator of the actin cytoskeleton. Knockdown of TAGLN decreases cell motility, whilst TAGLN overexpression rescues the motility defect resulting from HES6 knockdown. These findings indicate HES6 contributes to the pathogenesis of ARMSp by enhancing both proliferation and cell motility.

  20. "Tähesõdade" kuninganna Natalie Portman / Triin Thalheim

    Index Scriptorium Estoniae

    Thalheim, Triin, 1982-

    2005-01-01

    58-s Cannes'i filmifestival avati George Lucas'e viimase "Tähesõdade" filmiga "Star Wars : Episode III - Revenge of the Sith". Ühes peaosas mängib näitlejanna N. Portman. Lisaks ülevaade "Mehed Natalie orbiidil"

  1. Politsei jätkab Ühispanga juhtkonnaga seotud firma variisiku otsimist / Inno Tähismaa

    Index Scriptorium Estoniae

    Tähismaa, Inno, 1970-

    2004-01-01

    Politsei otsib Ühispanga juhtkonnaga seotud offshore-firmast Patent Developments Ltd. väidetavalt raha välja võtnud variisikut. Eesti Ühispanka rahapesus süüdistada pole võimalik, kuna 1997. aastal rahapesu tõkestamise seadus veel ei kehtinud

  2. Hey2 functions in parallel with Hes1 and Hes5 for mammalian auditory sensory organ development

    Directory of Open Access Journals (Sweden)

    Chin Michael T

    2008-02-01

    Full Text Available Abstract Background During mouse development, the precursor cells that give rise to the auditory sensory organ, the organ of Corti, are specified prior to embryonic day 14.5 (E14.5. Subsequently, the sensory domain is patterned precisely into one row of inner and three rows of outer sensory hair cells interdigitated with supporting cells. Both the restriction of the sensory domain and the patterning of the sensory mosaic of the organ of Corti involve Notch-mediated lateral inhibition and cellular rearrangement characteristic of convergent extension. This study explores the expression and function of a putative Notch target gene. Results We report that a putative Notch target gene, hairy-related basic helix-loop-helix (bHLH transcriptional factor Hey2, is expressed in the cochlear epithelium prior to terminal differentiation. Its expression is subsequently restricted to supporting cells, overlapping with the expression domains of two known Notch target genes, Hairy and enhancer of split homolog genes Hes1 and Hes5. In combination with the loss of Hes1 or Hes5, genetic inactivation of Hey2 leads to increased numbers of mis-patterned inner or outer hair cells, respectively. Surprisingly, the ectopic hair cells in Hey2 mutants are accompanied by ectopic supporting cells. Furthermore, Hey2-/-;Hes1-/- and Hey2-/-;Hes1+/- mutants show a complete penetrance of early embryonic lethality. Conclusion Our results indicate that Hey2 functions in parallel with Hes1 and Hes5 in patterning the organ of Corti, and interacts genetically with Hes1 for early embryonic development and survival. Our data implicates expansion of the progenitor pool and/or the boundaries of the developing sensory organ to account for patterning defects observed in Hey2 mutants.

  3. Hes1 and Hes3 regulate maintenance of the isthmic organizer and development of the mid/hindbrain

    Science.gov (United States)

    Hirata, Hiromi; Tomita, Koichi; Bessho, Yasumasa; Kageyama, Ryoichiro

    2001-01-01

    The isthmic organizer, which is located at the midbrain–hindbrain boundary, plays an essential role in development of the midbrain and anterior hindbrain. It has been shown that homeobox genes regulate establishment of the isthmic organizer, but the mechanism by which the organizer is maintained is not well understood. Here, we found that, in mice doubly mutant for the basic helix–loop–helix genes Hes1 and Hes3, the midbrain and anterior hindbrain structures are missing without any significant cell death. In these mutants, the isthmic organizer cells prematurely differentiate into neurons and terminate expression of secreting molecules such as Fgf8 and Wnt1 and the paired box genes Pax2/5, all of which are essential for the isthmic organizer function. These results indicate that Hes1 and Hes3 prevent premature differentiation and maintain the organizer activity of the isthmic cells, thereby regulating the development of the midbrain and anterior hindbrain. PMID:11500373

  4. Hes1 and Hes3 regulate maintenance of the isthmic organizer and development of the mid/hindbrain

    OpenAIRE

    Hirata, Hiromi; Tomita, Koichi; Bessho, Yasumasa; Kageyama, Ryoichiro

    2001-01-01

    The isthmic organizer, which is located at the midbrain–hindbrain boundary, plays an essential role in development of the midbrain and anterior hindbrain. It has been shown that homeobox genes regulate establishment of the isthmic organizer, but the mechanism by which the organizer is maintained is not well understood. Here, we found that, in mice doubly mutant for the basic helix–loop–helix genes Hes1 and Hes3, the midbrain and anterior hindbrain structures are missing without any significan...

  5. Results of NASA/NOAA HES Trade Studies

    Science.gov (United States)

    Susskind, Joel

    2011-01-01

    This slide presentation reviews the trade studies that were done for the Hyperspectral Environmental Suite (HES). The goal of the trade studies was to minimize instrument cost and risk while producing scientifically useful products. Three vendors were selected to perform the trade study, and were to conduct 11 studies, with the first study a complete wish list of things that scientists would like from GEO orbit to the 11th study which was for a Reduced Accommodation Sounder (RAS) which would still result in useful scientific products, within constrains compatible with flight on GEOS-R. The RAS's from each vendor and one other HES sounders designs are reviewed.

  6. Validity and Reliability of Korean Version of Health Empowerment Scale (K-HES for Older Adults

    Directory of Open Access Journals (Sweden)

    Chorong Park, MSN, RN

    2013-09-01

    Conclusion: The K-HES had acceptable validity and reliability. The brevity and ease of administration of the K-HES makes it a suitable tool for evaluating empowerment-based education programs targeted towards older populations.

  7. hesõdade edukas start / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    2002-01-01

    Tallinna kobarkino näitab alates 17. maist uut "Tähesõdade" filmi "Star Wars : osa II - Kloonide rünnak" ("Star Wars : Episode 2 - Attack of te Clones") : Ameerika Ühendriigid 2002. Maailma meedia vastukajadest Ameerika esilinastusele

  8. hesõjad on tagasi / Merit Kopli, Kaido Kopli

    Index Scriptorium Estoniae

    Kopli, Merit, 1969-

    1999-01-01

    George Lucas ja tema ulmefilmid "Tähesõjad", millest uusim "Star Wars I : The Phantom Menace" tõotab kujuneda 1999.aasta menukaimaks filmiks : Ajalehe lehekülg mitmesuguse infoga filmi maksumuse, tegelaste, režissööri ja esilinastuse kohta

  9. HES-SO SmartEnergy Project; Projet HES-SO SmartEnergy

    Energy Technology Data Exchange (ETDEWEB)

    Gabioud, D.

    2008-12-15

    This is the final report of a coordinated research project aiming at optimizing the operation of an electric power distribution network. The questions raised are: (i) What should the ideal load profile in the network be? (ii) Which pricing model should be implemented by the power distribution company in order to create adequate financial incentives for the consumer to modify his power consumption profile towards the ideal profile expected by the distribution company? (iii) What kind of information should be provided to the consumer to 'push' him towards this ideal load profile? The interdisciplinary two-years research project was conducted by four institutes of the University of Applied Sciences of Western Switzerland HES-SO in Sion, Sierre and Fribourg and the Centre for Energetic and Municipal Research CREM in Martigny. The first part of the report summarizes the project in French. It is followed by the slides presented at the workshop organised in Lausanne on November 28th, 2008 by the 'Smart Energy Steering Group'. The workshop was attended by 30 professionals (power distribution companies, power generation companies, equipment manufacturers, software developers, public utilities and researchers). The slides are written in French, English or German. It was concluded that dynamic pricing is able to significantly contribute to stabilize the electric network and to reduce the economic risk in the network operation. In particular, the operation of electric devices and machinery to generate heat and cold can be shifted to off-peak periods.

  10. Ärimees Armin Karult raha nõudnud "rullnokad" toimetasid politsei nina all / Rasmus Kagge, Risto Berendson

    Index Scriptorium Estoniae

    Kagge, Rasmus, 1977-

    2008-01-01

    Ilmunud ka: Postimees : na russkom jazõke 17. jaan. 2008, lk. 2-3. Raplamaa noorukeid Ardot ja Viljarit kahtlustatakse kasiinoärimehe Armin Karu tütre Inese pantvangi võtmise kava sepistamises, nad lootsid Olympic Casino keti suuromanikult saada 235 miljonit krooni lunaraha. Politsei tööst kuriteo avastamisel ja nurjamisel. Lisa: Inimröövid. Vt. samas: Armin Karu: kahju, et selliseid tegelasi leidub. Ilmunud ka: samal teemal:Risto Berendsoni art. 18. jaan. Postimees lk. 6; 19. jaan. Postimees ja Postimees : na russkom jazõke lk. 5 ja lk. 3

  11. Study of redundant Models in reliability prediction of HXMT's HES

    International Nuclear Information System (INIS)

    Wang Jinming; Liu Congzhan; Zhang Zhi; Ji Jianfeng

    2010-01-01

    Two redundant equipment structures of HXMT's HES are proposed firstly, the block backup and dual system cold-redundancy. Then prediction of the reliability is made by using parts count method. Research of comparison and analysis is also performed on the two proposals. A conclusion is drawn that a higher reliability and longer service life could be offered by taking a redundant equipment structure of block backup. (authors)

  12. Kiviõli politsei jättis hätta neli korda abi palunud mehe / Frank Sprenk-Dorn

    Index Scriptorium Estoniae

    Sprenk-Dorn, Frank

    2004-01-01

    Kiviõli lähistel Erra külas kolm autovarast kinni pidanud Mati Vilumets süüdistab politseid tema väljakutsetele reageerimata jätmises, politsei hinnangul ei olnud tema kõned üheselt mõistetavad. Kommenteerib Ida politseiprefekt Kalev Prillop

  13. Hes1 is required for appropriate morphogenesis and differentiation during mouse thyroid gland development.

    Directory of Open Access Journals (Sweden)

    Aurore Carre

    Full Text Available Notch signalling plays an important role in endocrine development, through its target gene Hes1. Hes1, a bHLH transcriptional repressor, influences progenitor cell proliferation and differentiation. Recently, Hes1 was shown to be expressed in the thyroid and regulate expression of the sodium iodide symporter (Nis. To investigate the role of Hes1 for thyroid development, we studied thyroid morphology and function in mice lacking Hes1. During normal mouse thyroid development, Hes1 was detected from E9.5 onwards in the median anlage, and at E11.5 in the ultimobranchial bodies. Hes1(-/- mouse embryos had a significantly lower number of Nkx2-1-positive progenitor cells (p<0.05 at E9.5 and at E11.5. Moreover, Hes1(-/- mouse embryos showed a significantly smaller total thyroid surface area (-40 to -60% compared to wild type mice at all study time points (E9.5-E16.5. In both Hes1(-/- and wild type mouse embryos, most Nkx2-1-positive thyroid cells expressed the cell cycle inhibitor p57 at E9.5 in correlation with low proliferation index. In Hes1(-/- mouse embryos, fusion of the median anlage with the ultimobranchial bodies was delayed by 3 days (E16.5 vs. E13.5 in wild type mice. After fusion of thyroid anlages, hypoplastic Hes1(-/- thyroids revealed a significantly decreased labelling area for T4 (-78% and calcitonin (-65% normalized to Nkx2-1 positive cells. Decreased T4-synthesis might be due to reduced Nis labelling area (-69%. These findings suggest a dual role of Hes1 during thyroid development: first, control of the number of both thyrocyte and C-cell progenitors, via a p57-independent mechanism; second, adequate differentiation and endocrine function of thyrocytes and C-cells.

  14. Periodic repression by the bHLH factor Hes7 is an essential mechanism for the somite segmentation clock

    Science.gov (United States)

    Bessho, Yasumasa; Hirata, Hiromi; Masamizu, Yoshito; Kageyama, Ryoichiro

    2003-01-01

    Hes7, a bHLH gene essential for somitogenesis, displays cyclic expression of mRNA in the presomitic mesoderm (PSM). Here, we show that Hes7 protein is also expressed in a dynamic manner, which depends on proteasome-mediated degradation. Spatial comparison revealed that Hes7 and Lunatic fringe (Lfng) transcription occurs in the Hes7 protein-negative domains. Furthermore, Hes7 and Lfng transcription is constitutively up-regulated in the absence of Hes7 protein and down-regulated by stabilization of Hes7 protein. Thus, periodic repression by Hes7 protein is critical for the cyclic transcription of Hes7 and Lfng, and this negative feedback represents a molecular basis for the segmentation clock. PMID:12783854

  15. Neural fate decisions mediated by combinatorial regulation of Hes1 and miR-9.

    Science.gov (United States)

    Li, Shanshan; Liu, Yanwei; Liu, Zengrong; Wang, Ruiqi

    2016-01-01

    In the nervous system, Hes1 shows an oscillatory manner in neural progenitors but a persistent one in neurons. Many models involving Hes1 have been provided for the study of neural differentiation but few of them take the role of microRNA into account. It is known that a microRNA, miR-9, plays crucial roles in modulating Hes1 oscillations. However, the roles of miR-9 in controlling Hes1 oscillations and inducing transition between different cell fates still need to be further explored. Here we provide a mathematical model to show the interaction between miR-9 and Hes1, with the aim of understanding how the Hes1 oscillations are produced, how they are controlled, and further, how they are terminated. Based on the experimental findings, the model demonstrates the essential roles of Hes1 and miR-9 in regulating the dynamics of the system. In particular, the model suggests that the balance between miR-9 and Hes1 plays important roles in the choice between progenitor maintenance and neural differentiation. In addition, the synergistic (or antagonistic) effects of several important regulations are investigated so as to elucidate the effects of combinatorial regulation in neural decision-making. Our model provides a qualitative mechanism for understanding the process in neural fate decisions regulated by Hes1 and miR-9.

  16. Periodic repression by the bHLH factor Hes7 is an essential mechanism for the somite segmentation clock

    OpenAIRE

    Bessho, Yasumasa; Hirata, Hiromi; Masamizu, Yoshito; Kageyama, Ryoichiro

    2003-01-01

    Hes7, a bHLH gene essential for somitogenesis, displays cyclic expression of mRNA in the presomitic mesoderm (PSM). Here, we show that Hes7 protein is also expressed in a dynamic manner, which depends on proteasome-mediated degradation. Spatial comparison revealed that Hes7 and Lunatic fringe (Lfng) transcription occurs in the Hes7 protein-negative domains. Furthermore, Hes7 and Lfng transcription is constitutively up-regulated in the absence of Hes7 protein and down-regulated by stabi...

  17. Balanced Hydroxyethylstarch (HES 130/0.4 Impairs Kidney Function In-Vivo without Inflammation.

    Directory of Open Access Journals (Sweden)

    Martin Alexander Schick

    Full Text Available Volume therapy is a standard procedure in daily perioperative care, and there is an ongoing discussion about the benefits of colloid resuscitation with hydroxyethylstarch (HES. In sepsis HES should be avoided due to a higher risk for acute kidney injury (AKI. Results of the usage of HES in patients without sepsis are controversial. Therefore we conducted an animal study to evaluate the impact of 6% HES 130/0.4 on kidney integrity with sepsis or under healthy conditions Sepsis was induced by standardized Colon Ascendens Stent Peritonitis (sCASP. sCASP-group as well as control group (C remained untreated for 24 h. After 18 h sCASP+HES group (sCASP+VOL and control+HES (C+VOL received 50 ml/KG balanced 6% HES (VOL 130/0.4 over 6 h. After 24 h kidney function was measured via Inulin- and PAH-Clearance in re-anesthetized rats, and serum urea, creatinine (crea, cystatin C and Neutrophil gelatinase-associated lipocalin (NGAL as well as histopathology were analysed. In vitro human proximal tubule cells (PTC were cultured +/- lipopolysaccharid (LPS and with 0.1-4.0% VOL. Cell viability was measured with XTT-, cell toxicity with LDH-test. sCASP induced severe septic AKI demonstrated divergent results regarding renal function by clearance or creatinine measure focusing on VOL. Soleley HES (C+VOL deteriorated renal function without sCASP. Histopathology revealed significantly derangements in all HES groups compared to control. In vitro LPS did not worsen the HES induced reduction of cell viability in PTC cells. For the first time, we demonstrated, that application of 50 ml/KG 6% HES 130/0.4 over 6 hours induced AKI without inflammation in vivo. Severity of sCASP induced septic AKI might be no longer susceptible to the way of volume expansion.

  18. The roles and mechanism of ultradian oscillatory expression of the mouse Hes genes.

    Science.gov (United States)

    Harima, Yukiko; Imayoshi, Itaru; Shimojo, Hiromi; Kobayashi, Taeko; Kageyama, Ryoichiro

    2014-10-01

    Somites, metameric structures, give rise to the vertebral column, ribs, skeletal muscles and subcutaneous tissues. In mouse embryos, a pair of somites is formed every 2h by segmentation of the anterior parts of the presomitic mesoderm. This periodic event is regulated by a biological clock called the segmentation clock, which involves cyclic expression of the basic helix-loop-helix gene Hes7. Hes7 oscillation is regulated by negative feedback with a delayed timing. This process has been mathematically simulated by differential-delay equations, which predict that negative feedback with shorter delays would abolish oscillations or produce dampened but more rapid oscillations. We found that reducing the number of introns within the Hes7 gene shortens the delay and abolishes Hes7 oscillation or results in a more rapid tempo of Hes7 oscillation, increasing the number of somites and vertebrae in the cervical and upper thoracic region. We also found that Hes1, a Hes7-related gene, is expressed in an oscillatory manner by many cell types, including fibroblasts and neural stem cells. In these cells, Hes1 expression oscillates with a period of about 2-3h, and this oscillation is important for cell cycle progression. Furthermore, in neural stem cells, Hes1 oscillation drives cyclic expression of the proneural genes Ascl1 and Neurogenin2 and regulates multipotency. Hes1 expression oscillates more slowly in embryonic stem cells, and Hes1 oscillation regulates their fate preferences. Taken together, these results suggest that oscillatory expression with short periods (ultradian oscillation) is important for many biological events. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Prospective observational study for perioperative volume replacement with 6% HES 130/0,42, 4% gelatin and 6% HES 200/0,5 in cardiac surgery

    Directory of Open Access Journals (Sweden)

    Winterhalter M

    2010-09-01

    Full Text Available Abstract Background The constantly growing amount of different kinds of colloid fluids necessitates comparative investigations with regards to the safety and effectivity in clinical use of these preparations. Hence we compared three colloid fluids in an observational study. The objective was the exploration of the influence of these three colloids on blood coagulation, hemodynamics and renal function of the cardiac surgical patient. Methods We included 90 patients undergoing an elective open-heart surgery with the use of the heart-lung machine and observed them consecutively. Group 1 [gelatin 4% (n = 30], Group 2 [HES 200/0,5 (n = 30] and Group 3 [HES 130/0,42 (n = 30]. We measured the perioperative volume replacement, the administration of blood- and coagulation-products, the application of catecholamines, the renal function, blood gas and the platelet aggregation using multiplate electrode analyzer (Multiplate®, Dynabyte medical, Munich, Germany. Results The gelatin-group needed significantly more norepinephrine than the HES 130/0.42 group. The responsible surgeon considered the blood coagulation in the HES 200/0.5 group most frequently as impaired. Furthermore we saw a significant decrease in platelet function in the HES 200/0.5 group when performing the multiplate®-analysis (ADP-and COL-test. HES 130/0.4 as well as gelatin 4% showed no significant change in platelet function. The gelatin-group and the HES 200/0.5 needed significantly more aprotinine than the HES 130/0.4 group. We saw no significant difference with regards to administration of blood and coagulation products between the three groups. The urinary excretion during the intervention was significantly higher in the HES 200/0.5 group and in the gelatin group than in the HES 130/0.4 group. Conclusions Our results confirm the lower stabilizing effect of gelatin on circulation during fluid resuscitation. The blood coagulation was mostly impaired due to HES 200/0.5 confirmed by the

  20. Research Outputs of England's Hospital Episode Statistics (HES) Database: Bibliometric Analysis.

    Science.gov (United States)

    Chaudhry, Zain; Mannan, Fahmida; Gibson-White, Angela; Syed, Usama; Ahmed, Shirin; Majeed, Azeem

    2017-12-06

    Hospital administrative data, such as those provided by the Hospital Episode Statistics (HES) database in England, are increasingly being used for research and quality improvement. To date, no study has tried to quantify and examine trends in the use of HES for research purposes. To examine trends in the use of HES data for research. Publications generated from the use of HES data were extracted from PubMed and analysed. Publications from 1996 to 2014 were then examined further in the Science Citation Index (SCI) of the Thompson Scientific Institute for Science Information (Web of Science) for details of research specialty area. 520 studies, categorised into 44 specialty areas, were extracted from PubMed. The review showed an increase in publications over the 18-year period with an average of 27 publications per year, however with the majority of output observed in the latter part of the study period. The highest number of publications was in the Health Statistics specialty area. The use of HES data for research is becoming more common. Increase in publications over time shows that researchers are beginning to take advantage of the potential of HES data. Although HES is a valuable database, concerns exist over the accuracy and completeness of the data entered. Clinicians need to be more engaged with HES for the full potential of this database to be harnessed.

  1. Hes1 Directly Controls Cell Proliferation through the Transcriptional Repression of p27Kip1

    Science.gov (United States)

    Murata, Kaoru; Hattori, Masakazu; Hirai, Norihito; Shinozuka, Yoriko; Hirata, Hiromi; Kageyama, Ryoichiro; Sakai, Toshiyuki; Minato, Nagahiro

    2005-01-01

    A transcriptional regulator, Hes1, plays crucial roles in the control of differentiation and proliferation of neuronal, endocrine, and T-lymphocyte progenitors during development. Mechanisms for the regulation of cell proliferation by Hes1, however, remain to be verified. In embryonic carcinoma cells, endogenous Hes1 expression was repressed by retinoic acid in concord with enhanced p27Kip1 expression and cell cycle arrest. Conversely, conditional expression of a moderate but not maximal level of Hes1 in HeLa cells by a tetracycline-inducible system resulted in reduced p27Kip1 expression, which was attributed to decreased basal transcript rather than enhanced proteasomal degradation, with concomitant increases in the growth rate and saturation density. Hes1 induction repressed the promoter activity of a 5′ flanking basal enhancer region of p27Kip1 gene in a manner dependent on Hes1 expression levels, and this was mediated by its binding to class C sites in the promoter region. Finally, hypoplastic fetal thymi, as well as livers and brains of Hes1-deficient mice, showed significantly increased p27Kip1 transcripts compared with those of control littermates. These results have suggested that Hes1 directly contributes to the promotion of progenitor cell proliferation through transcriptional repression of a cyclin-dependent kinase inhibitor, p27Kip1. PMID:15870295

  2. Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine

    International Nuclear Information System (INIS)

    Suzuki, Katsumasa; Fukui, Hirokazu; Kayahara, Takahisa; Sawada, Mitsutaka; Seno, Hiroshi; Hiai, Hiroshi; Kageyama, Ryoichiro; Okano, Hideyuki; Chiba, Tsutomu

    2005-01-01

    We have previously shown that Hes1 is expressed both in putative epithelial stem cells just above Paneth cells and in the crypt base columnar cells between Paneth cells, while Hes1 is completely absent in Paneth cells. This study was undertaken to clarify the role of Hes1 in Paneth cell differentiation, using Hes1-knockout (KO) newborn (P0) mice. Electron microscopy revealed premature appearance of distinct cells containing cytoplasmic granules in the intervillous region in Hes1-KO P0 mice, whereas those cells were absent in wild-type (WT) P0 mice. In Hes1-KO P0 mice, the gene expressions of cryptdins, exclusively present in Paneth cells, were all enhanced compared with WT P0 mice. Immunohistochemistry demonstrated increased number of both lysozyme-positive and cryptdin-4-positive cells in the small intestinal epithelium of Hes1-KO P0 mice as compared to WT P0 mice. Thus, Hes1 appears to have an inhibitory role in Paneth cell differentiation in the small intestine

  3. Epigenetic inactivation of Notch-Hes pathway in human B-cell acute lymphoblastic leukemia.

    Directory of Open Access Journals (Sweden)

    Shao-Qing Kuang

    Full Text Available The Notch pathway can have both oncogenic and tumor suppressor roles, depending on cell context. For example, Notch signaling promotes T cell differentiation and is leukemogenic in T cells, whereas it inhibits early B cell differentiation and acts as a tumor suppressor in B cell leukemia where it induces growth arrest and apoptosis. The regulatory mechanisms that contribute to these opposing roles are not understood. Aberrant promoter DNA methylation and histone modifications are associated with silencing of tumor suppressor genes and have been implicated in leukemogenesis. Using methylated CpG island amplification (MCA/DNA promoter microarray, we identified Notch3 and Hes5 as hypermethylated in human B cell acute lymphoblastic leukemia (ALL. We investigated the methylation status of other Notch pathway genes by bisulfite pyrosequencing. Notch3, JAG1, Hes2, Hes4 and Hes5 were frequently hypermethylated in B leukemia cell lines and primary B-ALL, in contrast to T-ALL cell lines and patient samples. Aberrant methylation of Notch3 and Hes5 in B-ALL was associated with gene silencing and was accompanied by decrease of H3K4 trimethylation and H3K9 acetylation and gain of H3K9 trimethylation and H3K27 trimethylation. 5-aza-2'-deoxycytidine treatment restored Hes5 expression and decreased promoter hypermethylation in most leukemia cell lines and primary B-ALL samples. Restoration of Hes5 expression by lentiviral transduction resulted in growth arrest and apoptosis in Hes5 negative B-ALL cells but not in Hes5 expressing T-ALL cells. These data suggest that epigenetic modifications are implicated in silencing of tumor suppressor of Notch/Hes pathway in B-ALL.

  4. Epigenetic inactivation of Notch-Hes pathway in human B-cell acute lymphoblastic leukemia.

    Science.gov (United States)

    Kuang, Shao-Qing; Fang, Zhihong; Zweidler-McKay, Patrick A; Yang, Hui; Wei, Yue; Gonzalez-Cervantes, Emilio A; Boumber, Yanis; Garcia-Manero, Guillermo

    2013-01-01

    The Notch pathway can have both oncogenic and tumor suppressor roles, depending on cell context. For example, Notch signaling promotes T cell differentiation and is leukemogenic in T cells, whereas it inhibits early B cell differentiation and acts as a tumor suppressor in B cell leukemia where it induces growth arrest and apoptosis. The regulatory mechanisms that contribute to these opposing roles are not understood. Aberrant promoter DNA methylation and histone modifications are associated with silencing of tumor suppressor genes and have been implicated in leukemogenesis. Using methylated CpG island amplification (MCA)/DNA promoter microarray, we identified Notch3 and Hes5 as hypermethylated in human B cell acute lymphoblastic leukemia (ALL). We investigated the methylation status of other Notch pathway genes by bisulfite pyrosequencing. Notch3, JAG1, Hes2, Hes4 and Hes5 were frequently hypermethylated in B leukemia cell lines and primary B-ALL, in contrast to T-ALL cell lines and patient samples. Aberrant methylation of Notch3 and Hes5 in B-ALL was associated with gene silencing and was accompanied by decrease of H3K4 trimethylation and H3K9 acetylation and gain of H3K9 trimethylation and H3K27 trimethylation. 5-aza-2'-deoxycytidine treatment restored Hes5 expression and decreased promoter hypermethylation in most leukemia cell lines and primary B-ALL samples. Restoration of Hes5 expression by lentiviral transduction resulted in growth arrest and apoptosis in Hes5 negative B-ALL cells but not in Hes5 expressing T-ALL cells. These data suggest that epigenetic modifications are implicated in silencing of tumor suppressor of Notch/Hes pathway in B-ALL.

  5. Test Report : GS Battery, EPC power HES RESCU

    Energy Technology Data Exchange (ETDEWEB)

    Rose, David Martin [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Schenkman, Benjamin L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Borneo, Daniel R. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2013-10-01

    The Department of Energy Office of Electricity (DOE/OE), Sandia National Laboratories (SNL) and the Base Camp Integration Lab (BCIL) partnered together to incorporate an energy storage system into a microgrid configured Forward Operating Base to reduce the fossil fuel consumption and to ultimately save lives. Energy storage vendors will be sending their systems to SNL Energy Storage Test Pad (ESTP) for functional testing and then to the BCIL for performance evaluation. The technologies that will be tested are electro-chemical energy storage systems comprising of lead acid, lithium-ion or zinc-bromide. GS Battery and EPC Power have developed an energy storage system that utilizes zinc-bromide flow batteries to save fuel on a military microgrid. This report contains the testing results and some limited analysis of performance of the GS Battery, EPC Power HES RESCU.

  6. Hes1 Is Required for Appropriate Morphogenesis and Differentiation during Mouse Thyroid Gland Development

    Science.gov (United States)

    Carre, Aurore; Rachdi, Latif; Tron, Elodie; Richard, Bénédicte; Castanet, Mireille; Schlumberger, Martin; Bidart, Jean-Michel

    2011-01-01

    Notch signalling plays an important role in endocrine development, through its target gene Hes1. Hes1, a bHLH transcriptional repressor, influences progenitor cell proliferation and differentiation. Recently, Hes1 was shown to be expressed in the thyroid and regulate expression of the sodium iodide symporter (Nis). To investigate the role of Hes1 for thyroid development, we studied thyroid morphology and function in mice lacking Hes1. During normal mouse thyroid development, Hes1 was detected from E9.5 onwards in the median anlage, and at E11.5 in the ultimobranchial bodies. Hes1 −/− mouse embryos had a significantly lower number of Nkx2-1-positive progenitor cells (p<0.05) at E9.5 and at E11.5. Moreover, Hes1 −/− mouse embryos showed a significantly smaller total thyroid surface area (−40 to −60%) compared to wild type mice at all study time points (E9.5−E16.5). In both Hes1 −/− and wild type mouse embryos, most Nkx2-1-positive thyroid cells expressed the cell cycle inhibitor p57 at E9.5 in correlation with low proliferation index. In Hes1 −/− mouse embryos, fusion of the median anlage with the ultimobranchial bodies was delayed by 3 days (E16.5 vs. E13.5 in wild type mice). After fusion of thyroid anlages, hypoplastic Hes1 −/− thyroids revealed a significantly decreased labelling area for T4 (−78%) and calcitonin (−65%) normalized to Nkx2-1 positive cells. Decreased T4-synthesis might be due to reduced Nis labelling area (−69%). These findings suggest a dual role of Hes1 during thyroid development: first, control of the number of both thyrocyte and C-cell progenitors, via a p57-independent mechanism; second, adequate differentiation and endocrine function of thyrocytes and C-cells. PMID:21364918

  7. Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics.

    Directory of Open Access Journals (Sweden)

    Andreas Sagner

    2018-02-01

    Full Text Available During tissue development, multipotent progenitors differentiate into specific cell types in characteristic spatial and temporal patterns. We addressed the mechanism linking progenitor identity and differentiation rate in the neural tube, where motor neuron (MN progenitors differentiate more rapidly than other progenitors. Using single cell transcriptomics, we defined the transcriptional changes associated with the transition of neural progenitors into MNs. Reconstruction of gene expression dynamics from these data indicate a pivotal role for the MN determinant Olig2 just prior to MN differentiation. Olig2 represses expression of the Notch signaling pathway effectors Hes1 and Hes5. Olig2 repression of Hes5 appears to be direct, via a conserved regulatory element within the Hes5 locus that restricts expression from MN progenitors. These findings reveal a tight coupling between the regulatory networks that control patterning and neuronal differentiation and demonstrate how Olig2 acts as the developmental pacemaker coordinating the spatial and temporal pattern of MN generation.

  8. Hes6 is required for actin cytoskeletal organization in differentiating C2C12 myoblasts

    Energy Technology Data Exchange (ETDEWEB)

    Malone, Caroline M.P.; Domaschenz, Renae; Amagase, Yoko [MRC Cancer Cell Unit, Hutchison-MRC Research Centre, Addenbrooke' s Hospital, Cambridge CB2 0XZ (United Kingdom); Dunham, Ian [EMBL-European Bioinformatics Institute (EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD (United Kingdom); Murai, Kasumi [MRC Cancer Cell Unit, Hutchison-MRC Research Centre, Addenbrooke' s Hospital, Cambridge CB2 0XZ (United Kingdom); Jones, Philip H., E-mail: phj20@cam.ac.uk [MRC Cancer Cell Unit, Hutchison-MRC Research Centre, Addenbrooke' s Hospital, Cambridge CB2 0XZ (United Kingdom)

    2011-07-01

    Hes6 is a member of the hairy-enhancer-of-split family of transcription factors that regulate proliferating cell fate in development and is known to be expressed in developing muscle. Here we investigate its function in myogenesis in vitro. We show that Hes6 is a direct transcriptional target of the myogenic transcription factors MyoD and Myf5, indicating that it is integral to the myogenic transcriptional program. The localization of Hes6 protein changes during differentiation, becoming predominantly nuclear. Knockdown of Hes6 mRNA levels by siRNA has no effect on cell cycle exit or induction of myosin heavy chain expression in differentiating C2C12 myoblasts, but F-actin filament formation is disrupted and both cell motility and myoblast fusion are reduced. The knockdown phenotype is rescued by expression of Hes6 cDNA resistant to siRNA. These results define a novel role for Hes6 in actin cytoskeletal dynamics in post mitotic myoblasts.

  9. A CREB-Sirt1-Hes1 Circuitry Mediates Neural Stem Cell Response to Glucose Availability

    Directory of Open Access Journals (Sweden)

    Salvatore Fusco

    2016-02-01

    Full Text Available Summary: Adult neurogenesis plays increasingly recognized roles in brain homeostasis and repair and is profoundly affected by energy balance and nutrients. We found that the expression of Hes-1 (hairy and enhancer of split 1 is modulated in neural stem and progenitor cells (NSCs by extracellular glucose through the coordinated action of CREB (cyclic AMP responsive element binding protein and Sirt-1 (Sirtuin 1, two cellular nutrient sensors. Excess glucose reduced CREB-activated Hes-1 expression and results in impaired cell proliferation. CREB-deficient NSCs expanded poorly in vitro and did not respond to glucose availability. Elevated glucose also promoted Sirt-1-dependent repression of the Hes-1 promoter. Conversely, in low glucose, CREB replaced Sirt-1 on the chromatin associated with the Hes-1 promoter enhancing Hes-1 expression and cell proliferation. Thus, the glucose-regulated antagonism between CREB and Sirt-1 for Hes-1 transcription participates in the metabolic regulation of neurogenesis. : Using a combination of in vitro and in vivo studies, Fusco et al. find that excess glucose impairs the self-renewal capacity of neural stem cells through a molecular circuit that involves the transcription factor CREB and Sirtuin 1. The authors suggest that this circuitry may link nutrient excess with neurodegeneration and brain aging. Keywords: neural stem cells, adult neurogenesis, CREB, Sirt-1, nutrients, metabolism, diabetes

  10. Hes6 is required for actin cytoskeletal organization in differentiating C2C12 myoblasts

    International Nuclear Information System (INIS)

    Malone, Caroline M.P.; Domaschenz, Renae; Amagase, Yoko; Dunham, Ian; Murai, Kasumi; Jones, Philip H.

    2011-01-01

    Hes6 is a member of the hairy-enhancer-of-split family of transcription factors that regulate proliferating cell fate in development and is known to be expressed in developing muscle. Here we investigate its function in myogenesis in vitro. We show that Hes6 is a direct transcriptional target of the myogenic transcription factors MyoD and Myf5, indicating that it is integral to the myogenic transcriptional program. The localization of Hes6 protein changes during differentiation, becoming predominantly nuclear. Knockdown of Hes6 mRNA levels by siRNA has no effect on cell cycle exit or induction of myosin heavy chain expression in differentiating C2C12 myoblasts, but F-actin filament formation is disrupted and both cell motility and myoblast fusion are reduced. The knockdown phenotype is rescued by expression of Hes6 cDNA resistant to siRNA. These results define a novel role for Hes6 in actin cytoskeletal dynamics in post mitotic myoblasts.

  11. Geostationary Operational Environmental Satellites (GOES): R series hyperspectral environmental suite (HES) overview

    Science.gov (United States)

    Martin, Gene; Criscione, Joseph C.; Cauffman, Sandra A.; Davis, Martin A.

    2004-11-01

    The Hyperspectral Environmental Suite (HES) instrument is currently under development by the NASA GOES-R Project team within the framework of the GOES Program to fulfill the future needs and requirements of the National Environmental Satellite, Data, and Information Service (NESDIS) Office. As part of the GOES-R instrument complement, HES will provide measurements of the traditional temperature and water vapor vertical profiles with higher accuracy and vertical resolution than obtained through current sounder technologies. HES will provide measurements of the properties of the shelf and coastal waters and back up imaging (at in-situ resolution) for the GOES-R Advanced Baseline Imager (ABI). The HES team is forging the future of remote environmental monitoring with the development of an operational instrument with high temporal, spatial and spectral-resolution and broad hemispheric coverage. The HES development vision includes threshold and goal requirements that encompass potential system solutions. The HES team has defined tasks for the instrument(s) that include a threshold functional complement of Disk Sounding (DS), Severe Weather/Mesoscale Sounding (SW/M), and Shelf and Coastal Waters imaging (CW) and a goal functional complement of Open Ocean (OO) imaging, and back up imaging (at in-situ resolution) for the GOES-R Advanced Baseline Imager (ABI). To achieve the best-value procurement, the GOES-R Project has base-lined a two-phase procurement approach to the HES design and development; a Formulation/study phase and an instrument Implementation phase. During Formulation, currently slated for the FY04-05 timeframe, the developing team(s) will perform Systems Requirements Analysis and evaluation, System Trade and Requirements Baseline Studies, Risk Assessment and Mitigation Strategy and complete a Preliminary Conceptual Design of the HES instrument. The results of the formulation phase will be leveraged to achieve an effective and efficient system solution during

  12. Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase.

    Directory of Open Access Journals (Sweden)

    Xi-Juan Liu

    2017-07-01

    Full Text Available Congenital human cytomegalovirus (HCMV infection is the leading cause of neurological disabilities in children worldwide, but the mechanisms underlying these disorders are far from well-defined. HCMV infection has been shown to dysregulate the Notch signaling pathway in human neural progenitor cells (NPCs. As an important downstream effector of Notch signaling, the transcriptional regulator Hairy and Enhancer of Split 1 (Hes1 is essential for governing NPC fate and fetal brain development. In the present study, we report that HCMV infection downregulates Hes1 protein levels in infected NPCs. The HCMV 72-kDa immediate-early 1 protein (IE1 is involved in Hes1 degradation by assembling a ubiquitination complex and promoting Hes1 ubiquitination as a potential E3 ubiquitin ligase, followed by proteasomal degradation of Hes1. Sp100A, an important component of PML nuclear bodies, is identified to be another target of IE1-mediated ubiquitination. A C-terminal acidic region in IE1, spanning amino acids 451 to 475, is required for IE1/Hes1 physical interaction and IE1-mediated Hes1 ubiquitination, but is dispensable for IE1/Sp100A interaction and ubiquitination. Our study suggests a novel mechanism linking downregulation of Hes1 protein to neurodevelopmental disorders caused by HCMV infection. Our findings also complement the current knowledge of herpesviruses by identifying IE1 as the first potential HCMV-encoded E3 ubiquitin ligase.

  13. Titaanide võitlus : "Sõrmuste isand" vs. "Tähesõjad" / Erkki Luuk

    Index Scriptorium Estoniae

    Luuk, Erkki, 1971-

    2002-01-01

    Esimene Peter Jacksoni käe all valmivast kolmest filmist J.R.R. Tolkieni triloogia "Sõrmuste isand" alusel - "Sõrmuse vennaskond", "The Lord of the Rings : The Fellowship of the Ring" : Uus-Meremaa, Ameerika Ühendriigid 2001. Arvustus on üles ehitatud filmi võrdlusele George Lucase epopöaga "Tähesõjad" ("Star Wars")

  14. Infot saab hallata kõik-ühes portaalitarkvaraga / Jaak Ennuste

    Index Scriptorium Estoniae

    Ennuste, Jaak

    2003-01-01

    Ilmunud ka: Delovõje Vedomosti 17. dets. lk. 23. Microsofti poolt turule toodud infoportaali tarkvara SharePoint Portal Server 2003 võimaldab kõiki infotöötlemise protsesse hallata ühes keskkonnas ja on mõeldud eelkõige informatsiooniga töötavatele ettevõtetele. Lisa: Portaali juurutamine ettevõttes

  15. Hes1 promotes the IL-22-mediated antimicrobial response by enhancing STAT3-dependent transcription in human intestinal epithelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Murano, Tatsuro [Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo (Japan); Okamoto, Ryuichi, E-mail: rokamoto.gast@tmd.ac.jp [Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo (Japan); Department of Advanced GI Therapeutics, Graduate School, Tokyo Medical and Dental University, Tokyo (Japan); Ito, Go; Nakata, Toru; Hibiya, Shuji; Shimizu, Hiromichi; Fujii, Satoru; Kano, Yoshihito; Mizutani, Tomohiro; Yui, Shiro; Akiyama-Morio, Junko; Nemoto, Yasuhiro [Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo (Japan); Tsuchiya, Kiichiro; Nakamura, Tetsuya [Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo (Japan); Department of Advanced GI Therapeutics, Graduate School, Tokyo Medical and Dental University, Tokyo (Japan); Watanabe, Mamoru [Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo (Japan)

    2014-01-17

    Highlights: •Hes1 enhances IL-22-STAT3 signaling in human intestinal epithelial cells. •Hes1 enhances REG family gene induction by IL-22-STAT3 signaling. •Protein level of Hes1 restricts the response to IL-22. •Present regulation of a cytokine signal represents a new mode of Hes1 function. -- Abstract: Notch signaling plays an essential role in the proliferation and differentiation of intestinal epithelial cells (IECs). We have previously shown that Notch signaling is up-regulated in the inflamed mucosa of ulcerative colitis (UC) and thereby plays an indispensable role in tissue regeneration. Here we show that in addition to Notch signaling, STAT3 signaling is highly activated in the inflamed mucosa of UC. Forced expression of the Notch target gene Hes1 dramatically enhanced the IL-22-mediated STAT3-dependent transcription in human IECs. This enhancement of STAT3-dependent transcription was achieved by the extended phosphorylation of STAT3 by Hes1. Microarray analysis revealed that Hes1-mediated enhancement of IL-22-STAT3 signaling significantly increased the induction of genes encoding antimicrobial peptides, such as REG1A, REG3A and REG3G, in human IECs. Conversely, the reduction of Hes1 protein levels with a γ-secretase inhibitor significantly down-regulated the induction of those genes in IECs, resulting in a markedly poor response to IL-22. Our present findings identify a new role for the molecular function of Hes1 in which the protein can interact with cytokine signals and regulate the immune response of IECs.

  16. Number of active transcription factor binding sites is essential for the Hes7 oscillator

    Directory of Open Access Journals (Sweden)

    de Angelis Martin

    2006-02-01

    Full Text Available Abstract Background It is commonly accepted that embryonic segmentation of vertebrates is regulated by a segmentation clock, which is induced by the cycling genes Hes1 and Hes7. Their products form dimers that bind to the regulatory regions and thereby repress the transcription of their own encoding genes. An increase of the half-life of Hes7 protein causes irregular somite formation. This was shown in recent experiments by Hirata et al. In the same work, numerical simulations from a delay differential equations model, originally invented by Lewis, gave additional support. For a longer half-life of the Hes7 protein, these simulations exhibited strongly damped oscillations with, after few periods, severely attenuated the amplitudes. In these simulations, the Hill coefficient, a crucial model parameter, was set to 2 indicating that Hes7 has only one binding site in its promoter. On the other hand, Bessho et al. established three regulatory elements in the promoter region. Results We show that – with the same half life – the delay system is highly sensitive to changes in the Hill coefficient. A small increase changes the qualitative behaviour of the solutions drastically. There is sustained oscillation and hence the model can no longer explain the disruption of the segmentation clock. On the other hand, the Hill coefficient is correlated with the number of active binding sites, and with the way in which dimers bind to them. In this paper, we adopt response functions in order to estimate Hill coefficients for a variable number of active binding sites. It turns out that three active transcription factor binding sites increase the Hill coefficient by at least 20% as compared to one single active site. Conclusion Our findings lead to the following crucial dichotomy: either Hirata's model is correct for the Hes7 oscillator, in which case at most two binding sites are active in its promoter region; or at least three binding sites are active, in which

  17. Bayesian inference for dynamic transcriptional regulation; the Hes1 system as a case study.

    Science.gov (United States)

    Heron, Elizabeth A; Finkenstädt, Bärbel; Rand, David A

    2007-10-01

    In this study, we address the problem of estimating the parameters of regulatory networks and provide the first application of Markov chain Monte Carlo (MCMC) methods to experimental data. As a case study, we consider a stochastic model of the Hes1 system expressed in terms of stochastic differential equations (SDEs) to which rigorous likelihood methods of inference can be applied. When fitting continuous-time stochastic models to discretely observed time series the lengths of the sampling intervals are important, and much of our study addresses the problem when the data are sparse. We estimate the parameters of an autoregulatory network providing results both for simulated and real experimental data from the Hes1 system. We develop an estimation algorithm using MCMC techniques which are flexible enough to allow for the imputation of latent data on a finer time scale and the presence of prior information about parameters which may be informed from other experiments as well as additional measurement error.

  18. Qingyihuaji Formula Inhibits Pancreatic Cancer and Prolongs Survival by Downregulating Hes-1 and Hey-1

    Directory of Open Access Journals (Sweden)

    Yanli Xu

    2015-01-01

    Full Text Available The dire prognosis of pancreatic cancer has not markedly improved during past decades. The present study was carried out to explore the effect of Qingyihuaji formula (QYHJ on inhibiting pancreatic cancer and prolonging survival in related Notch signaling pathway. Proliferation of pancreatic cancer cells (SW1990 and PANC-1 was detected by MTT assay at 24, 48, and 72 h with exposure to various concentrations (0.08–50 mg/mL of QYHJ water extract. Pancreatic tumor models of nude mice were divided into three groups randomly (control, QYHJ, and gemcitabine. mRNA and protein expression of Notch target genes (Hes-1, Hey-1, Hey-2, and Hey-L in dissected tumor tissue were detected. Results showed that proliferation of SW1990 cells and PANC-1 cells was inhibited by QYHJ water extract in a dose-dependent and time-dependent manner. QYHJ effectively inhibited tumor growth and prolonged survival time in nude mice. Expression of both Hes-1 and Hey-1 was decreased significantly in QYHJ group, suggesting that Hes-1 and Hey-1 in Notch signaling pathway might be potential targets for QYHJ treatment. This research could help explain the clinical effectiveness of QYHJ and may provide advanced pancreatic cancer patients with a new therapeutic option.

  19. HES1, a target of Notch signaling, is elevated in canine osteosarcoma, but reduced in the most aggressive tumors.

    Science.gov (United States)

    Dailey, Deanna D; Anfinsen, Kristin P; Pfaff, Liza E; Ehrhart, E J; Charles, J Brad; Bønsdorff, Tina B; Thamm, Douglas H; Powers, Barbara E; Jonasdottir, Thora J; Duval, Dawn L

    2013-07-01

    Hairy and enhancer of split 1 (HES1), a basic helix-loop-helix transcriptional repressor, is a downstream target of Notch signaling. Notch signaling and HES1 expression have been linked to growth and survival in a variety of human cancer types and have been associated with increased metastasis and invasiveness in human osteosarcoma cell lines. Osteosarcoma (OSA) is an aggressive cancer demonstrating both high metastatic rate and chemotherapeutic resistance. The current study examined expression of Notch signaling mediators in primary canine OSA tumors and canine and human osteosarcoma cell lines to assess their role in OSA development and progression. Reverse transcriptase - quantitative PCR (RT-qPCR) was utilized to quantify HES1, HEY1, NOTCH1 and NOTCH2 gene expression in matched tumor and normal metaphyseal bone samples taken from dogs treated for appendicular OSA at the Colorado State University Veterinary Teaching Hospital. Gene expression was also assessed in tumors from dogs with a disease free interval (DFI) of  300 days following treatment with surgical amputation followed by standard chemotherapy. Immunohistochemistry was performed to confirm expression of HES1. Data from RT-qPCR and immunohistochemical (IHC) experiments were analyzed using REST2009 software and survival analysis based on IHC expression employed the Kaplan-Meier method and log rank analysis. Unbiased clustered images were generated from gene array analysis data for Notch/HES1 associated genes. Gene array analysis of Notch/HES1 associated genes suggested alterations in the Notch signaling pathway may contribute to the development of canine OSA. HES1 mRNA expression was elevated in tumor samples relative to normal bone, but decreased in tumor samples from dogs with a DFI 300 days. NOTCH2 and HEY1 mRNA expression was also elevated in tumors relative to normal bone, but was not differentially expressed between the DFI tumor groups. Survival analysis confirmed an association between

  20. The role of the Hes1 crosstalk hub in Notch-Wnt interactions of the intestinal crypt

    KAUST Repository

    Kay, Sophie K.; Harrington, Heather A.; Shepherd, Sarah; Brennan, Keith; Dale, Trevor; Osborne, James M.; Gavaghan, David J.; Byrne, Helen M.

    2017-01-01

    The Notch pathway plays a vital role in determining whether cells in the intestinal epithelium adopt a secretory or an absorptive phenotype. Cell fate specification is coordinated via Notch’s interaction with the canonical Wnt pathway. Here, we propose a new mathematical model of the Notch and Wnt pathways, in which the Hes1 promoter acts as a hub for pathway crosstalk. Computational simulations of the model can assist in understanding how healthy intestinal tissue is maintained, and predict the likely consequences of biochemical knockouts upon cell fate selection processes. Chemical reaction network theory (CRNT) is a powerful, generalised framework which assesses the capacity of our model for monostability or multistability, by analysing properties of the underlying network structure without recourse to specific parameter values or functional forms for reaction rates. CRNT highlights the role of β-catenin in stabilising the Notch pathway and damping oscillations, demonstrating that Wnt-mediated actions on the Hes1 promoter can induce dynamic transitions in the Notch system, from multistability to monostability. Time-dependent model simulations of cell pairs reveal the stabilising influence of Wnt upon the Notch pathway, in which β-catenin- and Dsh-mediated action on the Hes1 promoter are key in shaping the subcellular dynamics. Where Notch-mediated transcription of Hes1 dominates, there is Notch oscillation and maintenance of fate flexibility; Wnt-mediated transcription of Hes1 favours bistability akin to cell fate selection. Cells could therefore regulate the proportion of Wnt- and Notch-mediated control of the Hes1 promoter to coordinate the timing of cell fate selection as they migrate through the intestinal epithelium and are subject to reduced Wnt stimuli. Furthermore, mutant cells characterised by hyperstimulation of the Wnt pathway may, through coupling with Notch, invert cell fate in neighbouring healthy cells, enabling an aberrant cell to maintain

  1. The role of the Hes1 crosstalk hub in Notch-Wnt interactions of the intestinal crypt

    KAUST Repository

    Kay, Sophie K.

    2017-03-01

    The Notch pathway plays a vital role in determining whether cells in the intestinal epithelium adopt a secretory or an absorptive phenotype. Cell fate specification is coordinated via Notch’s interaction with the canonical Wnt pathway. Here, we propose a new mathematical model of the Notch and Wnt pathways, in which the Hes1 promoter acts as a hub for pathway crosstalk. Computational simulations of the model can assist in understanding how healthy intestinal tissue is maintained, and predict the likely consequences of biochemical knockouts upon cell fate selection processes. Chemical reaction network theory (CRNT) is a powerful, generalised framework which assesses the capacity of our model for monostability or multistability, by analysing properties of the underlying network structure without recourse to specific parameter values or functional forms for reaction rates. CRNT highlights the role of β-catenin in stabilising the Notch pathway and damping oscillations, demonstrating that Wnt-mediated actions on the Hes1 promoter can induce dynamic transitions in the Notch system, from multistability to monostability. Time-dependent model simulations of cell pairs reveal the stabilising influence of Wnt upon the Notch pathway, in which β-catenin- and Dsh-mediated action on the Hes1 promoter are key in shaping the subcellular dynamics. Where Notch-mediated transcription of Hes1 dominates, there is Notch oscillation and maintenance of fate flexibility; Wnt-mediated transcription of Hes1 favours bistability akin to cell fate selection. Cells could therefore regulate the proportion of Wnt- and Notch-mediated control of the Hes1 promoter to coordinate the timing of cell fate selection as they migrate through the intestinal epithelium and are subject to reduced Wnt stimuli. Furthermore, mutant cells characterised by hyperstimulation of the Wnt pathway may, through coupling with Notch, invert cell fate in neighbouring healthy cells, enabling an aberrant cell to maintain

  2. The jagged-2/notch-1/hes-1 pathway is involved in intestinal epithelium regeneration after intestinal ischemia-reperfusion injury.

    Directory of Open Access Journals (Sweden)

    Guoqing Chen

    Full Text Available Notch signaling plays a critical role in the maintenance of intestinal crypt epithelial cell proliferation. The aim of this study was to investigate the role of Notch signaling in the proliferation and regeneration of intestinal epithelium after intestinal ischemia reperfusion (I/R injury.Male Sprague-Dawley rats were subjected to sham operation or I/R by occlusion of the superior mesenteric artery (SMA for 20 min. Intestinal tissue samples were collected at 0, 1, 2, 4, and 6 h after reperfusion. Proliferation of the intestinal epithelium was evaluated by immunohistochemical staining of proliferating nuclear antigen (PCNA. The mRNA and protein expression levels of Notch signaling components were examined using Real-time PCR and Western blot analyses. Immunofluorescence was also performed to detect the expression and location of Jagged-2, cleaved Notch-1, and Hes-1 in the intestine. Finally, the γ-secretase inhibitor DAPT and the siRNA for Jagged-2 and Hes-1 were applied to investigate the functional role of Notch signaling in the proliferation of intestinal epithelial cells in an in vitro IEC-6 culture system.I/R injury caused increased intestinal crypt epithelial cell proliferation and increased mRNA and protein expression of Jagged-2, Notch-1, and Hes-1. The immunofluorescence results further confirmed increased protein expression of Jagged-2, cleaved Notch-1, and Hes-1 in the intestinal crypts. The inhibition of Notch signaling with DAPT and the suppression of Jagged-2 and Hes-1 expression using siRNA both significantly inhibited the proliferation of IEC-6 cells.The Jagged-2/Notch-1/Hes-1 signaling pathway is involved in intestinal epithelium regeneration early after I/R injury by increasing crypt epithelial cell proliferation.

  3. The role of the Hes1 crosstalk hub in Notch-Wnt interactions of the intestinal crypt.

    Directory of Open Access Journals (Sweden)

    Sophie K Kay

    2017-02-01

    Full Text Available The Notch pathway plays a vital role in determining whether cells in the intestinal epithelium adopt a secretory or an absorptive phenotype. Cell fate specification is coordinated via Notch's interaction with the canonical Wnt pathway. Here, we propose a new mathematical model of the Notch and Wnt pathways, in which the Hes1 promoter acts as a hub for pathway crosstalk. Computational simulations of the model can assist in understanding how healthy intestinal tissue is maintained, and predict the likely consequences of biochemical knockouts upon cell fate selection processes. Chemical reaction network theory (CRNT is a powerful, generalised framework which assesses the capacity of our model for monostability or multistability, by analysing properties of the underlying network structure without recourse to specific parameter values or functional forms for reaction rates. CRNT highlights the role of β-catenin in stabilising the Notch pathway and damping oscillations, demonstrating that Wnt-mediated actions on the Hes1 promoter can induce dynamic transitions in the Notch system, from multistability to monostability. Time-dependent model simulations of cell pairs reveal the stabilising influence of Wnt upon the Notch pathway, in which β-catenin- and Dsh-mediated action on the Hes1 promoter are key in shaping the subcellular dynamics. Where Notch-mediated transcription of Hes1 dominates, there is Notch oscillation and maintenance of fate flexibility; Wnt-mediated transcription of Hes1 favours bistability akin to cell fate selection. Cells could therefore regulate the proportion of Wnt- and Notch-mediated control of the Hes1 promoter to coordinate the timing of cell fate selection as they migrate through the intestinal epithelium and are subject to reduced Wnt stimuli. Furthermore, mutant cells characterised by hyperstimulation of the Wnt pathway may, through coupling with Notch, invert cell fate in neighbouring healthy cells, enabling an aberrant

  4. Režissöör George Lucase loodud "Tähesõjad" elasid üle külma sõja / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    2007-01-01

    Mängufilmide triloogia "Tähesõjad" ("Star Wars") esimene film esilinastus 30 aastat tagasi. Filmi tähendustest läbi aja, nõukogude tsensuurist, mis ei lubanud filmi NSV Liitu. Lisa : "Fakte "Tähesõdadest"

  5. ÜhesHingamise peol Tallinnas on Pala valla kultuurisaadikuks kolm rahvatantsurühma / Jaan Lukas

    Index Scriptorium Estoniae

    Lukas, Jaan, 1964-

    2009-01-01

    2.- 5. juulini Tallinnas toimuval tantsupeol "ÜhesHingamine" esinevad Anna Haava nimelise Pala Kooli kaheksandate ja üheksandate klasside segarühm (juhendaja on kooli direktor Malle Weinrauch), Pala Kultuurimaja segarühm (juhendaja M. Weinrauch) ja Ranna naisrühm Lahe (juhendaja Julia Tross). Laulupeost võtab osa Pala Kultuurimaja segakoor

  6. The Notch/Hes1 pathway sustains NF-κB activation through CYLD repression in T cell leukemia.

    Science.gov (United States)

    Espinosa, Lluis; Cathelin, Severine; D'Altri, Teresa; Trimarchi, Thomas; Statnikov, Alexander; Guiu, Jordi; Rodilla, Veronica; Inglés-Esteve, Julia; Nomdedeu, Josep; Bellosillo, Beatriz; Besses, Carles; Abdel-Wahab, Omar; Kucine, Nicole; Sun, Shao-Cong; Song, Guangchan; Mullighan, Charles C; Levine, Ross L; Rajewsky, Klaus; Aifantis, Iannis; Bigas, Anna

    2010-09-14

    It was previously shown that the NF-κB pathway is downstream of oncogenic Notch1 in T cell acute lymphoblastic leukemia (T-ALL). Here, we visualize Notch-induced NF-κB activation using both human T-ALL cell lines and animal models. We demonstrate that Hes1, a canonical Notch target and transcriptional repressor, is responsible for sustaining IKK activation in T-ALL. Hes1 exerts its effects by repressing the deubiquitinase CYLD, a negative IKK complex regulator. CYLD expression was found to be significantly suppressed in primary T-ALL. Finally, we demonstrate that IKK inhibition is a promising option for the targeted therapy of T-ALL as specific suppression of IKK expression and function affected both the survival of human T-ALL cells and the maintenance of the disease in vivo. Copyright © 2010 Elsevier Inc. All rights reserved.

  7. Discovery of HeLa Cell Contamination in HES Cells: Call for Cell Line Authentication in Reproductive Biology Research.

    Science.gov (United States)

    Kniss, Douglas A; Summerfield, Taryn L

    2014-08-01

    Continuous cell lines are used frequently in reproductive biology research to study problems in early pregnancy events and parturition. It has been recognized for 50 years that many mammalian cell lines contain inter- or intraspecies contaminations with other cells. However, most investigators do not routinely test their culture systems for cross-contamination. The most frequent contributor to cross-contamination of cell lines is the HeLa cell isolated from an aggressive cervical adenocarcinoma. We report on the discovery of HeLa cell contamination of the human endometrial epithelial cell line HES isolated in our laboratory. Short tandem repeat analysis of 9 unique genetic loci demonstrated molecular identity between HES and HeLa cells. In addition, we verified that WISH cells, isolated originally from human amnion epithelium, were also contaminated with HeLa cells. Inasmuch as our laboratory did not culture HeLa cells at the time of HES cell derivations, the source of contamination was the WISH cell line. These data highlight the need for continued diligence in authenticating cell lines used in reproductive biology research. © The Author(s) 2014.

  8. Whole Genome Sequencing Identifies a Missense Mutation in HES7 Associated with Short Tails in Asian Domestic Cats.

    Science.gov (United States)

    Xu, Xiao; Sun, Xin; Hu, Xue-Song; Zhuang, Yan; Liu, Yue-Chen; Meng, Hao; Miao, Lin; Yu, He; Luo, Shu-Jin

    2016-08-25

    Domestic cats exhibit abundant variations in tail morphology and serve as an excellent model to study the development and evolution of vertebrate tails. Cats with shortened and kinked tails were first recorded in the Malayan archipelago by Charles Darwin in 1868 and remain quite common today in Southeast and East Asia. To elucidate the genetic basis of short tails in Asian cats, we built a pedigree of 13 cats segregating at the trait with a founder from southern China and performed linkage mapping based on whole genome sequencing data from the pedigree. The short-tailed trait was mapped to a 5.6 Mb region of Chr E1, within which the substitution c. 5T > C in the somite segmentation-related gene HES7 was identified as the causal mutation resulting in a missense change (p.V2A). Validation in 245 unrelated cats confirmed the correlation between HES7-c. 5T > C and Chinese short-tailed feral cats as well as the Japanese Bobtail breed, indicating a common genetic basis of the two. In addition, some of our sampled kinked-tailed cats could not be explained by either HES7 or the Manx-related T-box, suggesting at least three independent events in the evolution of domestic cats giving rise to short-tailed traits.

  9. Human embryonic stem cell (hES derived dendritic cells are functionally normal and are susceptible to HIV-1 infection

    Directory of Open Access Journals (Sweden)

    Bandi Sriram

    2008-01-01

    Full Text Available Abstract Background Human embryonic stem (hES cells hold considerable promise for cell replacement and gene therapies. Their remarkable properties of pluripotency, self-renewal, and tractability for genetic modification potentially allows for the production of sizeable quantities of therapeutic cells of the hematopoietic lineage. Dendritic cells (DC arise from CD34+ hematopoietic progenitor cells (HPCs and are important in many innate and adaptive immune functions. With respect to HIV-1 infection, DCs play an important role in the efficient capture and transfer of the virus to susceptible cells. With an aim of generating DCs from a renewable source for HIV-1 studies, here we evaluated the capacity of hES cell derived CD34+ cells to give rise to DCs which can support HIV-1 infection. Results Undifferentiated hES cells were cultured on S17 mouse bone marrow stromal cell layers to derive CD34+ HPCs which were subsequently grown in specific cytokine differentiation media to promote the development of DCs. The hES derived DCs (hES-DC were subjected to phenotypic and functional analyses and compared with DCs derived from fetal liver CD34+ HPC (FL-DC. The mature hES-DCs displayed typical DC morphology consisting of veiled stellate cells. The hES-DCs also displayed characteristic phenotypic surface markers CD1a, HLA-DR, B7.1, B7.2, and DC-SIGN. The hES-DCs were found to be capable of antigen uptake and stimulating naïve allogeneic CD4+ T cells in a mixed leukocyte reaction assay. Furthermore, the hES-DCs supported productive HIV-1 viral infection akin to standard DCs. Conclusion Phenotypically normal and functionally competent DCs that support HIV-1 infection can be derived from hES cells. hES-DCs can now be exploited in applied immunology and HIV-1 infection studies. Using gene therapy approaches, it is now possible to generate HIV-1 resistant DCs from anti-HIV gene transduced hES-CD34+ hematopoietic progenitor cells.

  10. Canine disorder mirrors human disease: exonic deletion in HES7 causes autosomal recessive spondylocostal dysostosis in miniature Schnauzer dogs.

    Directory of Open Access Journals (Sweden)

    Cali E Willet

    Full Text Available Spondylocostal dysostosis is a congenital disorder of the axial skeleton documented in human families from diverse racial backgrounds. The condition is characterised by truncal shortening, extensive hemivertebrae and rib anomalies including malalignment, fusion and reduction in number. Mutations in the Notch signalling pathway genes DLL3, MESP2, LFNG, HES7 and TBX6 have been associated with this defect. In this study, spondylocostal dysostosis in an outbred family of miniature schnauzer dogs is described. Computed tomography demonstrated that the condition mirrors the skeletal defects observed in human cases, but unlike most human cases, the affected dogs were stillborn or died shortly after birth. Through gene mapping and whole genome sequencing, we identified a single-base deletion in the coding region of HES7. The frameshift mutation causes loss of functional domains essential for the oscillatory transcriptional autorepression of HES7 during somitogenesis. A restriction fragment length polymorphism test was applied within the immediate family and supported a highly penetrant autosomal recessive mode of inheritance. The mutation was not observed in wider testing of 117 randomly sampled adult miniature schnauzer and six adult standard schnauzer dogs; providing a significance of association of Praw = 4.759e-36 (genome-wide significant. Despite this apparently low frequency in the Australian population, the allele may be globally distributed based on its presence in two unrelated sires from geographically distant locations. While isolated hemivertebrae have been observed in a small number of other dog breeds, this is the first clinical and genetic diagnosis of spontaneously occurring spondylocostal dysostosis in a non-human mammal and offers an excellent model in which to study this devastating human disorder. The genetic test can be utilized by dog breeders to select away from the disease and avoid unnecessary neonatal losses.

  11. Canine disorder mirrors human disease: exonic deletion in HES7 causes autosomal recessive spondylocostal dysostosis in miniature Schnauzer dogs.

    Science.gov (United States)

    Willet, Cali E; Makara, Mariano; Reppas, George; Tsoukalas, George; Malik, Richard; Haase, Bianca; Wade, Claire M

    2015-01-01

    Spondylocostal dysostosis is a congenital disorder of the axial skeleton documented in human families from diverse racial backgrounds. The condition is characterised by truncal shortening, extensive hemivertebrae and rib anomalies including malalignment, fusion and reduction in number. Mutations in the Notch signalling pathway genes DLL3, MESP2, LFNG, HES7 and TBX6 have been associated with this defect. In this study, spondylocostal dysostosis in an outbred family of miniature schnauzer dogs is described. Computed tomography demonstrated that the condition mirrors the skeletal defects observed in human cases, but unlike most human cases, the affected dogs were stillborn or died shortly after birth. Through gene mapping and whole genome sequencing, we identified a single-base deletion in the coding region of HES7. The frameshift mutation causes loss of functional domains essential for the oscillatory transcriptional autorepression of HES7 during somitogenesis. A restriction fragment length polymorphism test was applied within the immediate family and supported a highly penetrant autosomal recessive mode of inheritance. The mutation was not observed in wider testing of 117 randomly sampled adult miniature schnauzer and six adult standard schnauzer dogs; providing a significance of association of Praw = 4.759e-36 (genome-wide significant). Despite this apparently low frequency in the Australian population, the allele may be globally distributed based on its presence in two unrelated sires from geographically distant locations. While isolated hemivertebrae have been observed in a small number of other dog breeds, this is the first clinical and genetic diagnosis of spontaneously occurring spondylocostal dysostosis in a non-human mammal and offers an excellent model in which to study this devastating human disorder. The genetic test can be utilized by dog breeders to select away from the disease and avoid unnecessary neonatal losses.

  12. Inhibition of Notch1 promotes hedgehog signalling in a HES1-dependent manner in chondrocytes and exacerbates experimental osteoarthritis.

    Science.gov (United States)

    Lin, Neng-Yu; Distler, Alfiya; Beyer, Christian; Philipi-Schöbinger, Ariella; Breda, Silvia; Dees, Clara; Stock, Michael; Tomcik, Michal; Niemeier, Andreas; Dell'Accio, Francesco; Gelse, Kolja; Mattson, Mark P; Schett, Georg; Distler, Jörg Hw

    2016-11-01

    Notch ligands and receptors have recently been shown to be differentially expressed in osteoarthritis (OA). We aim to further elucidate the functional role of Notch signalling in OA using Notch1 antisense transgenic (Notch1 AS) mice. Notch and hedgehog signalling were analysed by real-time PCR and immunohistochemistry. Notch-1 AS mice were employed as a model of impaired Notch signalling in vivo. Experimental OA was induced by destabilisation of the medial meniscus (DMM). The extent of cartilage destruction and osteophyte formation was analysed by safranin-O staining with subsequent assessment of the Osteoarthritis Research Society International (OARSI) and Mankin scores and µCT scanning. Collagen X staining was used as a marker of chondrocyte hypertrophy. The role of hairy/enhancer of split 1 (Hes-1) was investigated with knockdown and overexpression experiments. Notch signalling was activated in human and murine OA with increased expression of Jagged1, Notch-1, accumulation of the Notch intracellular domain 1 and increased transcription of Hes-1. Notch1 AS mice showed exacerbated OA with increases in OARSI scores, osteophyte formation, increased subchondral bone plate density, collagen X and osteocalcin expression and elevated levels of Epas1 and ADAM-TS5 mRNA. Inhibition of the Notch pathway induced activation of hedgehog signalling with induction of Gli-1 and Gli-2 and increased transcription of hedgehog target genes. The regulatory effects of Notch signalling on Gli-expression were mimicked by Hes-1. Inhibition of Notch signalling activates hedgehog signalling, enhances chondrocyte hypertrophy and exacerbates experimental OA including osteophyte formation. These data suggest that the activation of the Notch pathway may limit aberrant hedgehog signalling in OA. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  13. The Evaluation of Hydroxyethyl Starch (6% HES 130/0.4 Solution’s Potential Preventive Effects on Coagulation Status in Women with Gynecologic Malignancies Using Rotation Thromboelastography

    Directory of Open Access Journals (Sweden)

    Meltem Olga Akay

    2014-09-01

    Full Text Available OBJECTIVE: The aim of this study was to determine the effects of in vitro hemodilution with 6% hydroxyethyl starch (HES 130/0.4 solution on the coagulation status of women with gynecologic malignancies by using rotation thromboelastogram (ROTEM®. METHODS: Twenty-two patients with gynecological tumors scheduled for anesthesia were enrolled. Blood samples were diluted by 20% with 6% HES (130/0.4 solution. RESULTS: In the INTEM assay, clotting time (CT (p<0.01 and clot formation time (CFT (p<0.001 were significantly increased and maximum maximum clot formation (MCF (p< 0.001 was significantly decreased in HES hemodilution compared with the undiluted control samples. In the EXTEM assay, there was a similar significant increase in increase in CFT (p<0.01 and a decrease in maximum a decrease in MCF (p<0.01 in HES hemodilution when compared with control samples. CONCLUSION: HES 130/0.4 solution causes significant hypocoagulable changes in the thromboelastographic profile of gynecologic cancer patients in vitro.

  14. Lung perfusion in hemorrhagic shock of rats. The effects of resuscitation with whole blood, saline or hes 6%

    Energy Technology Data Exchange (ETDEWEB)

    Turhanoglu, S.; Kaya, S.; Kararmaz, A.; Turhanoglu, A.D. [Dicle Univ., Diyarbakir (Turkey). Medical School

    2001-12-01

    This study was undertaken to determine the effects of various resuscitation regimens on lung perfusion following resuscitation from hemorrhagic shock. Fourty male Sprague-Dawley rats (250-300 g) were used. The rats were divided randomly into four groups (n=10 for each) and were sedated with intramuscular ketamine (100 mg/kg). We measured blood pressure, rectal temperature and lung perfusion using radioscintigraphy with a technetium colloid indicator. The systolic blood pressure was decreased 75% by removing blood via v. jugularis in the first three groups and group 4 was accepted as the control group, and blood volume was not diminished. Then the first three groups were resuscitated with autologous blood containing 125 units heparine/ml in group 1, saline in group 2, and hydroxyethyl starch (HES) 6% in group 3. After the correction of hypovolemia, all animals were injected 100 Bg (0.1 cc) technetium 99m macroaggregated albumin ({sup 99m}Tc MAA) via penil vein. After injection of {sup 99m}Tc MAA, 3 minutes fixed images were detected by a {gamma} camera in posterior position at 15 minutes and 5 hours. {sup 99m}Tc MMA ''wash out'' rate in lung was determined quantitatively at 5 hours. Compared to a control group, lung perfusion was decreased significantly in groups resuscitated with saline, and HES 6% while perfusion was restored with autologous blood. We conclude that heparinized autologous blood saved lung capillary circulation in hemorrhagic shock in rats. (author)

  15. Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage

    Directory of Open Access Journals (Sweden)

    Wei Zou

    2015-01-01

    Full Text Available Notch pathway activation maintains neural stem cells in a proliferating state and increases nerve repair capacity. To date, studies have rarely focused on changes or damage to signal transduction pathways during cerebral hemorrhage. Here, we examined the effect of acupuncture in a rat model of cerebral hemorrhage. We examined four groups: in the control group, rats received no treatment. In the model group, cerebral hemorrhage models were established by infusing non-heparinized blood into the brain. In the acupuncture group, modeled rats had Baihui (DU20 and Qubin (GB7 acupoints treated once a day for 30 minutes. In the DAPT group, modeled rats had 0.15 μg/mL DAPT solution (10 mL infused into the brain. Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia. These inhibitory effects were identical to DAPT, a Notch signaling pathway inhibitor. Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by inhibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage.

  16. Lung perfusion in hemorrhagic shock of rats. The effects of resuscitation with whole blood, saline or hes 6%

    International Nuclear Information System (INIS)

    Turhanoglu, S.; Kaya, S.; Kararmaz, A.; Turhanoglu, A.D.

    2001-01-01

    This study was undertaken to determine the effects of various resuscitation regimens on lung perfusion following resuscitation from hemorrhagic shock. Fourty male Sprague-Dawley rats (250-300 g) were used. The rats were divided randomly into four groups (n=10 for each) and were sedated with intramuscular ketamine (100 mg/kg). We measured blood pressure, rectal temperature and lung perfusion using radioscintigraphy with a technetium colloid indicator. The systolic blood pressure was decreased 75% by removing blood via v. jugularis in the first three groups and group 4 was accepted as the control group, and blood volume was not diminished. Then the first three groups were resuscitated with autologous blood containing 125 units heparine/ml in group 1, saline in group 2, and hydroxyethyl starch (HES) 6% in group 3. After the correction of hypovolemia, all animals were injected 100 Bg (0.1 cc) technetium 99m macroaggregated albumin ( 99m Tc MAA) via penil vein. After injection of 99m Tc MAA, 3 minutes fixed images were detected by a γ camera in posterior position at 15 minutes and 5 hours. 99m Tc MMA ''wash out'' rate in lung was determined quantitatively at 5 hours. Compared to a control group, lung perfusion was decreased significantly in groups resuscitated with saline, and HES 6% while perfusion was restored with autologous blood. We conclude that heparinized autologous blood saved lung capillary circulation in hemorrhagic shock in rats. (author)

  17. Tumour necrosis factor-alpha impairs neuronal differentiation but not proliferation of hippocampal neural precursor cells: Role of Hes1.

    Science.gov (United States)

    Keohane, Aoife; Ryan, Sinead; Maloney, Eimer; Sullivan, Aideen M; Nolan, Yvonne M

    2010-01-01

    Tumour necrosis factor-alpha (TNFalpha) is a pro-inflammatory cytokine, which influences neuronal survival and function yet there is limited information available on its effects on hippocampal neural precursor cells (NPCs). We show that TNFalpha treatment during proliferation had no effect on the percentage of proliferating cells prepared from embryonic rat hippocampal neurosphere cultures, nor did it affect cell fate towards either an astrocytic or neuronal lineage when cells were then allowed to differentiate. However, when cells were differentiated in the presence of TNFalpha, significantly reduced percentages of newly born and post-mitotic neurons, significantly increased percentages of astrocytes and increased expression of TNFalpha receptors, TNF-R1 and TNF-R2, as well as expression of the anti-neurogenic Hes1 gene, were observed. These data indicate that exposure of hippocampal NPCs to TNFalpha when they are undergoing differentiation but not proliferation has a detrimental effect on their neuronal lineage fate, which may be mediated through increased expression of Hes1. Copyright 2009 Elsevier Inc. All rights reserved.

  18. Bosnia - pelgupaik islamiradikaalidele? / Nicholas Wood ; tõlk. Krister Paris

    Index Scriptorium Estoniae

    Wood, Nicholas

    2006-01-01

    Bosnias avastas politsei 2005. aasta sügisel ühes korteris suure hulga relvi ning suitsiidivöösid, sellele on järgnenud mitmeid vahistamisi. Euroopa julgeolekuteenistused kardavad Bosnia muutumist terroristide baasiks

  19. Advanced glycation end product-induced astrocytic differentiation of cultured neurospheres through inhibition of Notch-Hes1 pathway-mediated neurogenesis.

    Science.gov (United States)

    Guo, Yijing; Wang, Pin; Sun, Haixia; Cai, Rongrong; Xia, Wenqing; Wang, Shaohua

    2013-12-23

    This study aims to investigate the roles of the Notch-Hes1 pathway in the advanced glycation end product (AGE)-mediated differentiation of neural stem cells (NSCs). We prepared pLentiLox3.7 lentiviral vectors that express short hairpin RNA (shRNA) against Notch1 and transfected it into NSCs. Cell differentiation was analyzed under confocal laser-scanning microscopy. The percentage of neurons and astrocytes was quantified by normalizing the total number of TUJ1+ (Neuron-specific class III β-tubulin) and GFAP+ (Glial fibrillary acidic protein) cells to the total number of Hoechst 33342-labeled cell nuclei. The protein and gene expression of Notch-Hes1 pathway components was examined via western blot analysis and real-time PCR. After 1 week of incubation, we found that AGE-bovine serum albumin (BSA) (400 μg/mL) induced the astrocytic differentiation of cultured neurospheres and inhibited neuronal formation. The expression of Notch-Hes1 pathway components was upregulated in the cells in the AGE-BSA culture medium. Immunoblot analysis indicated that shRNA silencing of Notch1 expression in NSCs significantly increases neurogenesis and suppresses astrocytic differentiation in NSCs incubated with AGE-BSA. AGEs promote the astrocytic differentiation of cultured neurospheres by inhibiting neurogenesis through the Notch-Hes1 pathway, providing a potential therapeutic target for hyperglycemia-related cognitive deficits.

  20. The management of pelvic organ prolapse in England: a 4-year analysis of hospital episode statistics (HES) data.

    Science.gov (United States)

    Ismail, S I F

    2014-08-01

    The aim of this study was to establish the number and trend of surgical procedures and pessary management of pelvic organ prolapse in England, using Hospital Episode Statistics (HES) data. An online search ( www.hesonline.nhs.uk ) was carried out. The number of various surgical procedures, as well as pessary insertions and removals for pelvic organ prolapse was obtained. Data were available for the 4-year period March 2002 to June 2005. Over 20,000 procedures performed and 600 pessaries inserted annually. The total number of patients having treatment for pelvic organ prolapse as well as the number of patients having surgery and pessary in English hospitals increased by data sooner and for longer periods as well as the use of more specific codes are needed to provide more useful information.

  1. The increasing number of surgical procedures for female genital fistula in England: analysis of Hospital Episode Statistics (HES) data.

    Science.gov (United States)

    Ismail, S I M F

    2015-01-01

    The aim of this study was to describe the number and trend of surgical procedures for female genital fistula in England. An online search of Hospital Episode Statistics (HES) data was carried out. Data were available for the 4-year period from 2002-03 until 2005-06. The total number of surgical procedures carried out for female genital fistula steadily increased by 28.7% from 616 in 2002-03 to 793 in 2005-06. The number of surgical procedures performed for rectovaginal fistula exceeded the total number of surgical procedures carried out for vesicovaginal and urethrovaginal fistula in each year of the study period. This pattern needs to be monitored and investigated further.

  2. Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately.

    Science.gov (United States)

    Shi, Yan; Shu, Bin; Yang, Ronghua; Xu, Yingbin; Xing, Bangrong; Liu, Jian; Chen, Lei; Qi, Shaohai; Liu, Xusheng; Wang, Peng; Tang, Jinming; Xie, Julin

    2015-06-16

    Wnt and Notch signaling pathways are critically involved in relative cell fate decisions within the development of cutaneous tissues. Moreover, several studies identified the above two pathways as having a significant role during wound healing. However, their biological effects during cutaneous tissues repair are unclear. We employed a self-controlled model (Sprague-Dawley rats with full-thickness skin wounds) to observe the action and effect of Wnt/β-catenin and Notch signalings in vivo. The quality of wound repair relevant to the gain/loss-of-function Wnt/β-catenin and Notch activation was estimated by hematoxylin-and-eosin and Masson staining. Immunofluorescence analysis and Western blot analysis were used to elucidate the underlying mechanism of the regulation of Wnt and Notch signaling pathways in wound healing. Meanwhile, epidermal stem cells (ESCs) were cultured in keratinocyte serum-free medium with Jaggedl or in DAPT (N-[(3,5-difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl) to investigate whether the interruption of Notch signaling contributes to the expression of Wnt/β-catenin signaling. The results showed that in vivo the gain-of-function Wnt/β-catenin and Notch activation extended the ability to promote wound closure. We further determined that activation or inhibition of Wnt signaling and Notch signaling can affect the proliferation of ESCs, the differentiation and migration of keratinocytes, and follicle regeneration by targeting c-Myc and Hes1, which ultimately lead to enhanced or delayed wound healing. Furthermore, Western blot analysis suggested that the two pathways might interact in vivo and in vitro. These results suggest that Wnt and Notch signalings play important roles in cutaneous repair by targeting c-Myc and Hes1 separately. What's more, interaction between the above two pathways might act as a vital role in regulation of wound healing.

  3. Report on Integration of Existing Grid Models for N-R HES Interaction Focused on Balancing Authorities for Sub-hour Penalties and Opportunities

    International Nuclear Information System (INIS)

    McJunkin, Timothy; Epiney, Aaron; Rabiti, Cristian

    2017-01-01

    This report provides a summary of the effort in the Nuclear-Renewable Hybrid Energy System (N-R HES) project on the level 4 milestone to consider integration of existing grid models into the factors for optimization on shorter time intervals than the existing electric grid models with the Risk Analysis Virtual Environment (RAVEN) and Modelica [1] optimizations and economic analysis that are the focus of the project to date.

  4. Open radical cystectomy in England: the current standard of care - an analysis of the British Association of Urological Surgeons (BAUS) cystectomy audit and Hospital Episodes Statistics (HES) data.

    Science.gov (United States)

    Jefferies, Edward R; Cresswell, Joanne; McGrath, John S; Miller, Catherine; Hounsome, Luke; Fowler, Sarah; Rowe, Edward W

    2018-01-23

    To establish the current standard for open radical cystectomy (ORC) in England, as data entry by surgeons performing RC to the British Association of Urological Surgeons (BAUS) database was mandated in 2013 and combining this with Hospital Episodes Statistics (HES) data has allowed comprehensive outcome analysis for the first time. All patients were included in this analysis if they were uploaded to the BAUS data registry and reported to have been performed in the 2 years between 1 January 2014 and 31 December 2015 in England (from mandate onwards) and had been documented as being performed in an open fashion (not laparoscopic, robot assisted or the technique field left blank). The HES data were accessed via the HES website. Office of Population Censuses and Surveys Classification of Surgical Operations and Procedures version 4 (OPCS-4) Code M34 was searched during the same 2-year time frame (not including M34.4 for simple cystectomy or with additional minimal access codes Y75.1-9 documenting a laparoscopic or robotic approach was used) to assess data capture. A total of 2 537 ORCs were recorded in the BAUS registry and 3 043 in the HES data. This indicates a capture rate of 83.4% of all cases. The median operative time was 5 h, harvesting a median of 11-20 lymph nodes, with a median blood loss of 500-1 000 mL, and a transfusion rate of 21.8%. The median length of stay was 11 days, with a 30-day mortality rate of 1.58%. This is the largest, contemporary cohort of ORCs in England, encompassing >80% of all performed operations. We now know the current standard for ORC in England. This provides the basis for individual surgeons and units to compare their outcomes and a standard with which future techniques and modifications can be compared. © 2018 The Authors BJU International © 2018 BJU International Published by John Wiley & Sons Ltd.

  5. Report on Integration of Existing Grid Models for N-R HES Interaction Focused on Balancing Authorities for Sub-hour Penalties and Opportunities

    Energy Technology Data Exchange (ETDEWEB)

    McJunkin, Timothy [Idaho National Lab. (INL), Idaho Falls, ID (United States); Epiney, Aaron [Idaho National Lab. (INL), Idaho Falls, ID (United States); Rabiti, Cristian [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2017-06-01

    This report provides a summary of the effort in the Nuclear-Renewable Hybrid Energy System (N-R HES) project on the level 4 milestone to consider integration of existing grid models into the factors for optimization on shorter time intervals than the existing electric grid models with the Risk Analysis Virtual Environment (RAVEN) and Modelica [1] optimizations and economic analysis that are the focus of the project to date.

  6. HES and T'Sou-ke Nation awarded CanSIA solar project of the year

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2010-01-15

    Home Energy Solutions (HES) has installed the largest solar photovoltaic solar array in British Columbia. In 2009, the Sum-SHA-Thut installation won the award for the Solar Project of the Year by the Canadian Solar Industries Association (CanSIA). It was built for Vancouver Island's T'Sou-ke Nation and its success shows great promise for solar electric generation in Canada. Energy consumption has decreased 30 per cent since the installation in July 2009, making the T'Sou-ke First Nation the most solar-intensive nation per capita in the world. The solar community project demonstrates that as a clean, renewable source of energy, solar electricity is a real solution for climate change, both internationally and at a local level. The timing of the CanSIA award is significant for the Canadian solar industry, falling in the midst of the Copenhagen Climate Change Conference and just shortly after Ontario introduced its micro feed-in-tariff program for renewable energy technologies. The project also demonstrates British Columbia's leadership in the alternative energy sector, and how solar can play a significant role in the future energy supply. 1 fig.

  7. Epigenetic silencing of miRNA-9 is associated with HES1 oncogenic activity and poor prognosis of medulloblastoma

    Science.gov (United States)

    Fiaschetti, G; Abela, L; Nonoguchi, N; Dubuc, A M; Remke, M; Boro, A; Grunder, E; Siler, U; Ohgaki, H; Taylor, M D; Baumgartner, M; Shalaby, T; Grotzer, M A

    2014-01-01

    Background: microRNA-9 is a key regulator of neuronal development aberrantly expressed in brain malignancies, including medulloblastoma. The mechanisms by which microRNA-9 contributes to medulloblastoma pathogenesis remain unclear, and factors that regulate this process have not been delineated. Methods: Expression and methylation status of microRNA-9 in medulloblastoma cell lines and primary samples were analysed. The association of microRNA-9 expression with medulloblastoma patients' clinical outcome was assessed, and the impact of microRNA-9 restoration was functionally validated in medulloblastoma cells. Results: microRNA-9 expression is repressed in a large subset of MB samples compared with normal fetal cerebellum. Low microRNA-9 expression correlates significantly with the diagnosis of unfavourable histopathological variants and with poor clinical outcome. microRNA-9 silencing occurs via cancer-specific CpG island hypermethylation. HES1 was identified as a direct target of microRNA-9 in medulloblastoma, and restoration of microRNA-9 was shown to trigger cell cycle arrest, to inhibit clonal growth and to promote medulloblastoma cell differentiation. Conclusions: microRNA-9 is a methylation-silenced tumour suppressor that could be a potential candidate predictive marker for poor prognosis of medulloblastoma. Loss of microRNA-9 may confer a proliferative advantage to tumour cells, and it could possibly contribute to disease pathogenesis. Thus, re-expression of microRNA-9 may constitute a novel epigenetic regulation strategy against medulloblastoma. PMID:24346283

  8. Epigenetic silencing of miRNA-9 is associated with HES1 oncogenic activity and poor prognosis of medulloblastoma.

    Science.gov (United States)

    Fiaschetti, G; Abela, L; Nonoguchi, N; Dubuc, A M; Remke, M; Boro, A; Grunder, E; Siler, U; Ohgaki, H; Taylor, M D; Baumgartner, M; Shalaby, T; Grotzer, M A

    2014-02-04

    microRNA-9 is a key regulator of neuronal development aberrantly expressed in brain malignancies, including medulloblastoma. The mechanisms by which microRNA-9 contributes to medulloblastoma pathogenesis remain unclear, and factors that regulate this process have not been delineated. Expression and methylation status of microRNA-9 in medulloblastoma cell lines and primary samples were analysed. The association of microRNA-9 expression with medulloblastoma patients' clinical outcome was assessed, and the impact of microRNA-9 restoration was functionally validated in medulloblastoma cells. microRNA-9 expression is repressed in a large subset of MB samples compared with normal fetal cerebellum. Low microRNA-9 expression correlates significantly with the diagnosis of unfavourable histopathological variants and with poor clinical outcome. microRNA-9 silencing occurs via cancer-specific CpG island hypermethylation. HES1 was identified as a direct target of microRNA-9 in medulloblastoma, and restoration of microRNA-9 was shown to trigger cell cycle arrest, to inhibit clonal growth and to promote medulloblastoma cell differentiation. microRNA-9 is a methylation-silenced tumour suppressor that could be a potential candidate predictive marker for poor prognosis of medulloblastoma. Loss of microRNA-9 may confer a proliferative advantage to tumour cells, and it could possibly contribute to disease pathogenesis. Thus, re-expression of microRNA-9 may constitute a novel epigenetic regulation strategy against medulloblastoma.

  9. Ülesande lahendamise eest saab miljon dollarit / Harli Uljas

    Index Scriptorium Estoniae

    Uljas, Harli

    2000-01-01

    Maailma tippmatemaatikutele pakutakse seitset miljonit dollarit seitsme raske võrduse lahendamise eest. Clay Mathematics Institute pani probleemi üles oma internetileheküljele ja teatas sellest Pariisis toimunud konverentsil. Princetoni ülikooli matemaatikaprofessori Andrew Wilesi sõnul on need seitse matemaatilist probleemi selle ala kõige suuremad lahendamata väljakutsed

  10. Hypereosinophilic Syndrome (HES)

    Science.gov (United States)

    ... Tubes Health Information Sheet Q & A with Experts Patient Stories Social Security Disability Application Process For Kids For Teens Managing EAD at College Patient Stories For Caregivers Questions to Ask Your doctor ...

  11. Clinical Application of colored three-dimensional CT (3D-CT) for brain tumors using helical scanning CT (HES-CT)

    International Nuclear Information System (INIS)

    Ogura, Yuko; Katada, Kazuhiro; Fujisawa, Kazuhisa; Imai, Fumihiro; Kawase, Tsukasa; Kamei, Yoshifumi; Kanno, Tetsuo; Takeshita, Gen; Koga, Sukehiko

    1995-01-01

    We applied colored three-dimensional CT (colored 3D-CT) images to distinguish brain tumors from the surrounding vascular and bony structures using a work station system and helical scanning CT (HES-CT). CT scanners with a slip-ring system were employed (TCT-900S and X vigor). A slice thickness of 2 mm and bed speed of 2 mm/s were used. The volume of contrast medium injected was 60 to 70 ml. Four to 8 colors were used for the tissue segmentation on the workstation system (xtension) using the data transferred from HES-CT. Tissue segmentation succeeded on the colored 3D-CT images in all 13 cases. The relationship between the tumors and the surrounding structures were easily recognized. The technique was useful to simulate operative fields, because deep structures could be visualized by cutting and drilling the colored 3D-CT volumetric data. On the basis of our findings, we suggest that colored 3D-CT images should be used as a supplementary aid for preoperative simulation. (author)

  12. Job characteristics in relation to the prevalence of myocardial infarction in the US Health Examination Survey (HES) and the Health and Nutrition Examination Survey (HANES).

    Science.gov (United States)

    Karasek, R A; Theorell, T; Schwartz, J E; Schnall, P L; Pieper, C F; Michela, J L

    1988-08-01

    Associations between psychosocial job characteristics and past myocardial infarction (MI) prevalence for employed males were tested with the Health Examination Survey (HES) 1960-61, N = 2,409, and the Health and Nutrition Examination Survey (HANES) 1971-75, N = 2,424. A new estimation method is used which imputes to census occupation codes, job characteristic information from national surveys of job characteristics (US Department of Labor, Quality of Employment Surveys). Controlling for age, we find that employed males with jobs which are simultaneously low in decision latitude and high in psychological work load (a multiplicative product term isolating 20 per cent of the population) have a higher prevalence of myocardial infarction in both data bases. In a logistic regression analysis, using job measures adjusted for demographic factors and controlling for age, race, education, systolic blood pressure, serum cholesterol, smoking (HANES only), and physical exertion, we find a low decision latitude/high psychological demand multiplicative product term associated with MI in both data bases. Additional multiple logistic regressions show that low decision latitude is associated with increased prevalence of MI in both the HES and the HANES. Psychological workload and physical exertion are significant only in the HANES.

  13. Notch 1 Receptor, Delta 1 Ligand and HES 1 Transcription Factor are Expressed in the Lining Epithelium of Periapical Cysts (Preliminary Study).

    Science.gov (United States)

    Meliou, E; Kerezoudis, Np; Tosios, Ki; Kiaris, H

    2010-07-27

    Periapical cyst is a chronic inflammatory disorder of periradicular tissues. The precise pathological mechanisms involved in periapical cyst enlargement remain unclear. Notch signaling is an evolutionarily conserved pathway with a regulatory role in cell fate decisions during development and in carcinogenesis. To date, there are no published data available on the expression of Notch signaling components in periapical cysts or any other jaw cyst. In this immunohistochemical study we have examined the expression of the receptor Notch 1, the ligand Delta 1 and the transcription factor HES 1 in the epithelium of well defined periapical cysts. Immunostaining reaction of Notch 1, Delta 1 and HES 1 was observed in the cytoplasm and/or the cytoplasmic membrane and occasionally in the nucleus in the majority of epithelial cells of all periapical cysts. The present observations indicate that Notch pathway is active in the epithelium of periapical cysts. It can be speculated that activation of epithelial cells of periapical cysts is associated with activation of Notch pathway and imply involvement of this pathway in periapical cyst growth and expansion.

  14. Saksa politsei testib imerelva / ref. Aadu Hiietamm

    Index Scriptorium Estoniae

    2004-01-01

    Saksa politseinikud testivad relva (Air Taser), mis ei tapa, kuuli asemel tabab ohvrit elektrilöök. Seni pole Saksamaa politseijuhid elektripüstoli ametlikuks kasutusele võtuks veel luba andnud, USA-s on relv aga kasutusel

  15. Politsei filmis vallajuht Lease vahistamist / Anneli Ammas

    Index Scriptorium Estoniae

    Ammas, Anneli, 1962-

    2005-01-01

    Kihnu vallavanem Johannes Leas sõlmis 1. juulil ehitusfirmaga OÜ Globetrotten kaks lepingut, vallavalitsust teavitas sellest aga 4. juulil. 130 000 krooni, mis väidetavalt oli J. Leasi vahistamisel tema autos, oli autori sõnul tõenäoliselt osa valla ettemaksust ehitusfirmale. Lisa: Kihnu ehitustööd küsimärgi all

  16. Acid-base and electrolyte status during normovolemic hemodilution with succinylated gelatin or HES-containing volume replacement solutions in rats.

    Directory of Open Access Journals (Sweden)

    Johanna K Teloh

    Full Text Available BACKGROUND: In the past, several studies have compared different colloidal replacement solutions, whereby the focus was usually on the respective colloid. We therefore systematically studied the influence of the carrier solution's composition of five approved colloidal volume replacement solutions (Gelafundin, Gelafusal, Geloplasma, Voluven and Volulyte on acid-base as well as electrolyte status during and following acute severe normovolemic hemodilution. The solutions differed in the colloid used (succinylated gelatin vs. HES and in the presence and concentration of metabolizable anions as well as in their electrolyte composition. METHODS: Anesthetized Wistar rats were subjected to a stepwise normovolemic hemodilution with one of the solutions until a final hematocrit of 10%. Subsequent to dilution (162 min, animals were observed for an additional period (150 min. During dilution and observation time blood gas analyses were performed eight times in total. Additionally, in the Voluven and Volulyte groups as well as in 6 Gelafundin animals, electrolyte concentrations, glucose, pH and succinylated gelatin were measured in urine and histopathological evaluation of the kidney was performed. RESULTS: All animals survived without any indications of injury. Although the employed solutions differed in their respective composition, comparable results in all plasma acid-base and electrolyte parameters studied were obtained. Plasma pH increased from approximately 7.28 to 7.39, the plasma K(+ concentration decreased from circa 5.20 mM to 4.80-3.90 mM and the plasma Cl(- concentration rose from approximately 105 mM to 111-120 mM. Urinary analysis revealed increased excretion of K(+, H(+ and Cl(-. CONCLUSIONS: The present data suggest that the carrier solution's composition with regard to metabolizable anions as well as K(+, Ca(2+ only has a minor impact on acid-base and electrolyte status after application of succinylated gelatin or HES-containing colloidal

  17. Evaluation des bibliothèques HES, sélection et définition d'indicateurs de performance et de qualité: répondre à une directive

    OpenAIRE

    Bezençon, Christophe; Gorin, Michel

    2009-01-01

    La Commission spécialisée des bibliothèques HES (CBH), organe de la Conférence des recteurs d es hautes écoles spécialisées suisses (KFH), a identifié la nécessité, dans le contexte académique actuel, de joindre à son projet de Directive pour la bibliothèque HES une collection d’indicateurs de performance et de qualité pour permettre aux bibliothèques de mesurer leur degré d’accomplissement des exigences issues de cette Directive. La sélection, la définition et l’explicitation de ces indicate...

  18. O mito das Olimpíadas: Hesíodo, Bacon, Hobbes e a infindável luta dos titãs

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Santos da Silva

    2016-08-01

    Full Text Available O presente artigo tem por objetivo apresentar alguns dos aspectos mitológicos que fundamentam a realização das olimpíadas desde a antiguidade grega, particularmente a “corrida de tochas acessas” que atualmente inaugura a abertura dos jogos. Para tanto, buscamos apresentar alguns relatos que narram a realização dos jogos olímpicos como uma espécie memória aos mitos fundadores da cultura grega. Nesse registro, consideramos alguns aspectos das narrativas de Hesíodo sobre o mito de Prometeu, bem como algumas observações de filósofos modernos como Francis Bacon e Thomas Hobbes sobre como essa fábula narra a interpretação de uma condição humana fundada em uma competição análoga à uma interminável luta de titãs. Condição humana essa oriunda da desobediência às vontades dos deuses do Olimpo, cristalizada na expressão de uma cultura da competição que visa não apenas agradar a esses deuses, detentores do poder no Olimpo, mas também igualar certos homens a eles.

  19. Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells.

    Directory of Open Access Journals (Sweden)

    Dai-tze Wu

    Full Text Available The identification of stem cells within a mixed population of cells is a major hurdle for stem cell biology--in particular, in the identification of induced pluripotent stem (iPS cells during the reprogramming process. Based on the selective expression of stem cell surface markers, a method to specifically infect stem cells through antibody-conjugated lentiviral particles has been developed that can deliver both visual markers for live-cell imaging as well as selectable markers to enrich for iPS cells. Antibodies recognizing SSEA4 and CD24 mediated the selective infection of the iPS cells over the parental human fibroblasts, allowing for rapid expansion of these cells by puromycin selection. Adaptation of the vector allows for the selective marking of human embryonic stem (hES cells for their removal from a population of differentiated cells. This method has the benefit that it not only identifies stem cells, but that specific genes, including positive and negative selection markers, regulatory genes or miRNA can be delivered to the targeted stem cells. The ability to specifically target gene delivery to human pluripotent stem cells has broad applications in tissue engineering and stem cell therapies.

  20. El "Escudo de Heracles" de Hesíodo: écfrasis y narración en la épica arcaica

    Directory of Open Access Journals (Sweden)

    David Konstan

    2001-12-01

    Full Text Available No "Escudo de Héracles" e no carmen 64 de Catulo (o "epitalâmio" de Peleu e Tetis, a écfrasis ocupa praticamente a metade do poema. Embora a de Catulo descreva como Teseu abandona Ariadne e consista, dessa forma, numa narrativa paralela à história principal, o escudo hesiódico não é uma narrativa propriamente dita e, sim, uma sequência de imagens distintas que existem simultaneamente, como em um desenho. Este artigo examina como a écfrasis hesiódica detém o movimento do tempo narrativo e apresenta uma comparação detalhada do "Escudo de Héracles" com a descrição do escudo de Aquiles na Ilíada. Embora ambas as écfrases suspendam o tempo narrativo, a de Hesíodo é mais fiel à simultaneidade das artes plásticas.

  1. Short curcumin treatment modulates oxidative stress, arginase activity, aberrant crypt foci, and TGF-β1 and HES-1 transcripts in 1,2-dimethylhydrazine-colon carcinogenesis in mice

    International Nuclear Information System (INIS)

    Bounaama, Abdelkader; Djerdjouri, Bahia; Laroche-Clary, Audrey; Le Morvan, Valérie; Robert, Jacques

    2012-01-01

    Highlights: ► 1,2-Dimethylhydrazine (DMH) toxicity was driven by oxidative stress. ► Arginase activity correlated to aberrant crypt foci (ACF). ► Curcumin diet restored redox status and induced apoptosis of dysplastic ACF. ► Curcumin reduced arginase activity and up regulated TGF-β1 and HES-1 transcripts. -- Abstract: This study investigated the effect of short curcumin treatment, a natural antioxidant on 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) in mice. The incidence of aberrant crypt foci (ACF) was 100%, with 54 ± 6 per colon, 10 weeks after the first DMH injection and reached 67 ± 12 per colon after 12 weeks. A high level of undifferentiated goblet cells and a weak apoptotic activity were shown in dysplastic ACF. The morphological alterations of colonic mucosa were associated to severe oxidative stress ratio with 43% increase in malondialdehyde vs. 36% decrease in GSH. DMH also increased inducible nitric synthase (iNOS) mRNA transcripts (250%), nitrites level (240%) and arginase activity (296%), leading to nitrosative stress and cell proliferation. Curcumin treatment, starting at week 10 post-DMH injection for 14 days, reduced the number of ACF (40%), iNOS expression (25%) and arginase activity (73%), and improved redox status by approximately 46%, compared to DMH-treated mice. Moreover, curcumin induced apoptosis of dysplastic ACF cells without restoring goblet cells differentiation. Interestingly, curcumin induced a parallel increase in TGF-β1 and HES-1 transcripts (42% and 26%, respectively). In conclusion, the protective effect of curcumin was driven by the reduction of arginase activity and nitrosative stress. The up regulation of TGF-β1 and HES-1 expression by curcumin suggests for the first time, a potential interplay between these signalling pathways in the chemoprotective mechanism of curcumin.

  2. Nõudmised tööjõule ja koolitajate ülesanded / Jaanus Kiili

    Index Scriptorium Estoniae

    Kiili, Jaanus, 1958-

    2001-01-01

    21. sajandi tööjõule vajalikest pädevustest, tehnikaerialade spetsialistidele esitatavatest nõuetest, ametialasest koolitusest keskastme ja kõrgtaseme tehnikaerialade spetsialistidele, tehnikaerialade täiend- ja ümberõppest

  3. Juuru Mihkli Kiriku Oreli Fond võttis endale uue ülesande / Tauno Kibur

    Index Scriptorium Estoniae

    Kibur, Tauno

    2009-01-01

    Oreli fond on võtnud ülesandeks restaureerida koguduse ajalooline pastoraat. Hoone eskiisi tegi Raivi Juks. Oreli restaureerimist ja tulemust jääb fikseerima Juuru oreli plaat, mille muusikapalad mängis sisse Kristel Aer

  4. Autoriõiguse seaduse eesmärk ja ülesanded / Heiki Pisuke

    Index Scriptorium Estoniae

    Pisuke, Heiki, 1955-

    2012-01-01

    20 aastat autoriõiguse seaduse vastuvõtmisest. Autoriõiguse seaduse algsest (RT I 1992, 49, 615), kehtivast (1999, 97, 859) ning võimalikust tulevasest redaktsioonist. Autorsusest ja õigusest autorsusele

  5. Politsei on tsiviilühiskonna lihased / Ignar Fjuk ; interv. Peep Lillemägi

    Index Scriptorium Estoniae

    Fjuk, Ignar, 1953-

    2001-01-01

    Ilmunud ka: Nädaline, 18. sept. 2001, lk. 6; Meie Maa, 18. sept. 2001, lk. 2; Virumaa Teataja, 20. sept. 2001, lk. 7; Valgamaalane, 20. sept. 2001, lk. 2; Vooremaa, 25. sept. 2001, lk. 2. Hinnang politseireformi sildi all toimunud koondamistele. Autor: Reformierakond. Parlamendisaadik

  6. Politsei pani Itaalia luurajatel CIA abistamise eest käed raudu / Olli Vesala

    Index Scriptorium Estoniae

    Vesala, Olli

    2006-01-01

    Itaalia prokurörid käskisid arreteerida kaks kõrget luureametnikku süüdistatuna Milanos CIA poolt terrorismis kahtlustatava islamivaimuliku röövimises kaasaaitamises. Lisa: Terrorismis kahtlustatu

  7. Politsei otsis Anders Tsahkna kodu läbi / Inno Tähismaa

    Index Scriptorium Estoniae

    Tähismaa, Inno, 1970-

    2004-01-01

    Seoses kriminaalmenetlusega selgitamaks Tallinna Diagnostikakeskuse 66,3 protsendi aktsiate võõrandamist OÜ-le Diagnostic Family, tehti 16. septembril 2004 Tallinna mitmes kontoris, sh A. Tsahkna kodus läbiotsimine

  8. Korruptsioonihõng : politsei teeb Narvas suurpuhastust / Tanel Mazur, Kärt Anvelt

    Index Scriptorium Estoniae

    Mazur, Tanel, 1971-

    2008-01-01

    Seoses 2007. aasta augustis alustatud kriminaalasjaga otsis kaitsepolitsei Narvas läbi ka abilinnapea Sofia Homjakova kabineti, kriminaalasja raames pole kellelegi kahtlustusi esitatud. Vt. samas: Ärimehed: Ida-Virumaal maksab iga liigutus

  9. Rotary klubi premeeris Politsei- ja Piirivalveameti töötajaid

    Index Scriptorium Estoniae

    2012-01-01

    Tallinna Rotary klubi noorte politseinike ning parima koerajuhi ja teenistuskoera preemia võitnutest: Raili Pärn, Marit Abram, Valur Pajumäe koeraga Golttvizen Hof Dixon, Hendri Lilbok ja Martin Torim

  10. The Glucocorticoid Receptor Controls Hepatic Dyslipidemia through Hes1

    NARCIS (Netherlands)

    Lemke, U.; Krones-Herzig, A.; Berriel Diaz, M.; Narvekar, P.; Ziegler, A.; Vegiopoulos, A.; Cato, A.C.B.; Bohl, S.; Klingmüller, U.; Screaton, R.A.; Müller-Decker, K.; Kersten, A.H.; Herzig, S.

    2008-01-01

    Aberrant accumulation of lipids in the liver (¿fatty liver¿ or hepatic steatosis) represents a hallmark of the metabolic syndrome and is tightly associated with obesity, type II diabetes, starvation, or glucocorticoid (GC) therapy. While fatty liver has been connected with numerous abnormalities of

  11. Case report 432: Granulocytic sarcoma (GS), with hypereosinophilic syndrome (HES)

    Energy Technology Data Exchange (ETDEWEB)

    Xipell, J M; Beamish, M R; Clark, D

    1987-07-01

    A case is presented of a 28-year-old man with a history of the hypereosinophilic syndrome, who subsequently developed granulocytic sarcoma of the tibia which proved resistant to aggressive therapy. He ultimately developed acute myeloid leukemia which also was resistant to therapy. The disease process was characterized by localised skeletal pain, the development of lytic lesions in several areas of the skeleton and progression to frank myeloid leukemia. (orig./SHA).

  12. Nokia tuleb ühes muusikaga / Kersti Saar

    Index Scriptorium Estoniae

    Saar, Kersti

    2008-01-01

    Nokia plaanib avada uut teenust Comes with Music, mis avab kliendile ligipääsu suurimate plaadifirmade Universal Music Croupi muusikakataloogidele. Diagramm: Nokia aktsia hind. Lisa: Comes with Music

  13. Hematology Expert System (HES) For Tonsillectomy/Adenoidectomy Patients

    Science.gov (United States)

    Pizzi, Nicolino J.; Kapoor, Sandhya; Gerrard, Jon M.

    1989-03-01

    The purpose of this expert system is to assess a predisposition to bleeding in a patient undergoing a tonsillectomy and/or adenoidectomy as may occur with patients who have certain blood conditions such as hemophilia and von Willebrand's disease. This goal is achieved by establishing a correlation between the patients' responses to a medical questionnaire and the relative quantities of blood lost during the operation.

  14. Õpetamine esi- ja algõpetuses : Alusväärtused. Ülesanded ja eesmärgid. Orientatsioonidest õppeaineteni. Metoodilised tervikud. Hindamine

    Index Scriptorium Estoniae

    2001-01-01

    Esi- ja algõpetuse alusväärtused ja eesmärgid. Esiõpetuse teadmisala-orientatsioonid ja algõpetuse õppeained. Mängu tähtsusest väikelaste õppimises, õppetunni planeerimine, integreeritud õpetus algõpetuses. Hindamine esi- ja algõpetuses

  15. Kuula. Kolm meest jälle ühes paadis / Mart Juur

    Index Scriptorium Estoniae

    Juur, Mart, 1964-

    2007-01-01

    Ansamblist The Police. Heliplaatidest: Bon Jovi "Lost Highway", Ray LaMontagne "Till The Sun Turns Black", The Bravery "The Sun And The Moon", Mark Ronson "Version", "Make Some Noise. The Campaign To Save Darfur"

  16. Dos Hesíodes per a una teogonia: mitologia nacional catalana

    Directory of Open Access Journals (Sweden)

    Magí Sunyer

    2014-06-01

    fostered a better understanding among Catalans of their own history and which, above all, provided the foundation on which Catalonia could be characterized as an intrinsically democratic country. This said, they also gave importance to Catalan medieval institutions (and to those who defended them when any king tried to devalue them as well as justifying the rebellious character of the Catalans. This can be seen in revolts against injustice and absolutism such as the war with John II, the Segadors war, and the War of the Spanish Succession, as well as in phenomena such as banditry.

  17. Debate on HES safety is important, but must be based on facts

    DEFF Research Database (Denmark)

    Haase, Nicolai; Müller, Rasmus; Perner, Anders

    2013-01-01

    The Scandinavian Starch for Severe Sepsis / Septic Shock (6S) trial showed that hydroxyethyl starch was harmful compared to Ringer's acetate in patients with severe sepsis when used according to clinical practice and in alignment with the recommendations by the manufactures and authorities. The d...

  18. Eesti Põlevkivi juht on monopolist ja kuningas ühes isikus / Lembit Kaljuvee ; interv. Andres Reimer

    Index Scriptorium Estoniae

    Kaljuvee, Lembit, 1952-

    2006-01-01

    Eesti Põlevkivi juhi arvates ei peaks uusi maardlaid avatama ning Eesti Põlevkivi võiks jätkata monopolina. Eesti Põlevkivi on mõelnud Venemaal Slantsõs tootmise peale ning kavatseb hõivata kolmandiku Eesti killustikuturust. Lisa: Kroonimata kuninga tähtpäev

  19. Taevas pole ainult tähesõdade paik / Reet Varblane

    Index Scriptorium Estoniae

    Varblane, Reet, 1952-

    2007-01-01

    Palmse mõisas 20.-27. V toimunud rahvusvahelisest põlisrahvaste kultuurifestivalist "Põhjataeva peegeldused". Tekstiilikunstnikke kureeris Kadri Viires, seppi Kaupo Kangur. Töötubades tehtu on väljas sepikojas. Jean-Loup Rousselot pidas loengutsükli Põhjala kultuurist, mõtlemisest ja mütoloogiast. Yinka Shonibare projektist "Armastuse aed" Pariisis kultuuride dialoogi muuseumis

  20. Kolm ühes : uhke kirik, võimla ja kontserdisaal / Eda Post

    Index Scriptorium Estoniae

    Post, Eda, 1983-

    2009-01-01

    Rakvere Pauluse kiriku (arhitekt Alar Kotli, 1930-ndate 2. pool) Arvo Pärdi nimeliseks muusikamajaks rekonstrueerimise ja Vabaduse väljaku planeerimise rahvusvahelisest arhitektuurivõistlusest, võidutööst "Tintin'na.buli", mille autorid on Kristiina Aasvee, Kristiina Hussar ja Anne Kose OÜ-st Celander Projekt. 2. koha sai töö "Hortus Musicus", mille autorid on Emil Urbel ja Aleksandr Zverev arhitektuuribüroost Emil Urbel OÜ

  1. Valitsused on ühes voodis ärimeestega / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Ligi 30 aastat tagasi loodud kosmeetika firma The Body Shop asutajast Anita Roddick'ust. Vt. samas: Minu suurim dilemma. Graafik: The Body Shopi aktsia on Londoni börsil näinud nii häid kui ka halbu aegu. Kommenteerib Meelis Milder

  2. Kas ühiskondliku heaolu eest hoolitsemine on tegelikult politsei ülesanne? Sekkuva halduse õiguslikud alused / Illimar Pärnamägi

    Index Scriptorium Estoniae

    Pärnamägi, Illimar

    2016-01-01

    Haldusõiguse rollist riikliku hoiaku ja käitumise aluseks oleva õigusharuna. Sekkuva halduse lähtekohtadest, liigsest sekkumisest tingitud võimalikest riskidest ja riskide maandamise võimalustest

  3. Police chief : homicide down, crime rate constant in 2004 / Aleksei Gunter

    Index Scriptorium Estoniae

    Gunter, Aleksei, 1979-

    2005-01-01

    Politseiameti teatel avastati 2004. aastal rekordarv kuritegusid. Politseiameti peadirektor Robert Antropov politseitööst ja ümberkorraldustest politseis 2004. aastal. Lisa: Politsei prioriteedid 2004 ja 2005 aastal

  4. Verine lugu Tallinna abilinnapea minevikust / Sulev Vedler

    Index Scriptorium Estoniae

    Vedler, Sulev, 1970-

    2011-01-01

    1999. a. plahvatas Mihhail Kõlvarti, Tallinna praeguse abilinnapea autos pomm, läbiotsimisel leiti tema kodunt laskemoona, kärbik, hulk politsei vormiriideid jm. Politsei kahtlustas Kõlvartit seotuses allilmaga

  5. Inglismaal hävitatakse fotoraamatuid

    Index Scriptorium Estoniae

    1998-01-01

    Mapplethorpe'i fotoraamatute konfiskeerimisest Birminghami Ülikooli raamatukogust Birminghami politsei poolt. Politsei on teinud avalduse kirjastusele Random House'ile, et hävitada veel laos olevad fotoalbumid

  6. Government liquidates secretariat

    Index Scriptorium Estoniae

    2008-01-01

    Läti valitsus kaotab kulude kokkuhoidmiseks Euroopa Liidu fondide sekretariaadi ja Euroopa Liidu Informatsiooni Agentuuri. Nende asutuste ülesanded delegeeritakse rahandusministeeriumile ja Riigikantseleile

  7. Lastekaitse kohaliku omavalitsuse tasandil : [bakalaureusetöö] / Ave Juhandi ; Õigusinstituut ; juhendaja: Eve Liblik

    Index Scriptorium Estoniae

    Juhandi, Ave

    2000-01-01

    Kohaliku omavalitsuse ülesanded laste hoolekande korraldamisel lähtudes Eesti Vabariigis kehtivatest õigusaktidest, laste hoolekanne Tallinna linnas, Tallinna Sotsiaal- ja tervishoiuameti haldusalasse kuuluvad laste hoolekandeasutused

  8. Kaks mõrva ühes mesilasperes ehk laibarida ei tule / Lauri Tabur ; interv. Enno Tammer

    Index Scriptorium Estoniae

    Tabur, Lauri, 1974-

    2005-01-01

    Keskkriminaalpolitsei ülem vastab küsimustele, mis puudutavad Tallinna koolitüdruku Veronika Dari, eestikeelse allilma väidetava liidri Kalev Kure ning ärimees Kauri Koppeli mõrvu Tallinnas. Lisa: Kiirarvamus

  9. Polizei-Compliance / Frank Braun, Florian Albrecht

    Index Scriptorium Estoniae

    Braun, Frank

    2015-01-01

    Normide ja seaduste järgimisest politsei tegevuses ning vastavussüsteemi juurutamisest politseiorganisatsioonis tegevuse läbipaistvamaks muutmiseks, halduse optimeerimiseks ja kodanike usalduse suurendamiseks

  10. "Isegi pankrotis Kreeka ei kaotanud politseinike eripensione, miks meie siis ometi seda teeme?" / Marvel Riik

    Index Scriptorium Estoniae

    Riik, Marvel

    2016-01-01

    Valitsus otsustas kaotada alates 2020. aastast kaitseväe, prokuröride, politsei ja piirivalveametnike eripensionid. Arvamust avaldavad reservkolonelleitnant Leo Kunnas ja ekspolitseinik Aivar Toompere

  11. Kriminaalpolitsei tulevikust / Arved Ant, Rauno Võsaste

    Index Scriptorium Estoniae

    Ant, Arved

    2006-01-01

    Kriminaalpolitsei hetkeolukorrast ja arenguvõimalustest, töökorralduse muutmisest, probleemikesksest tegutsemisest ning kriminaalpolitseinike motiveerimisest. Tabel: Politsei, kaitsepolitsei ja prokuratuuri palgaastmete võrdlustabel

  12. Strateegia ja suurte plaanide lõpp / John Kay

    Index Scriptorium Estoniae

    Kay, John

    2001-01-01

    Äristrateegia ettevõttes - selle tekkimine, sisu, ülesanded. Tänapäevane ressursipõhine vaade strateegiale, mis põhineb majandustrendi, konkurentsieelise ja eristavate ning jäljendatavate võimete kontseptsioonidel

  13. Kool noorte õigusliku sotsialiseerimise agendina : [bakalaureusetöö] / Heleri Kivi ; Tartu Ülikool, õigusteaduskond ; juhendaja: Silvia Kaugia

    Index Scriptorium Estoniae

    Kivi, Heleri

    2007-01-01

    Õigusliku sotsialiseerimise olemus ja agendid, kooli eripära noorte õigusliku sotsialiseerimise agentide hulgas, koolivägivald probleemide osundajana, kooli ülesanded noorte õigusliku sotsialiseerumise tõhustamisel

  14. Eraõiguslik sotsiaalõigus: vastutuse muutumine sotsiaalse rehabilitatsiooni õigussuhete kolmnurgas / Mari-Liis Viirsalu

    Index Scriptorium Estoniae

    Viirsalu, Mari-Liis, 1983-

    2017-01-01

    Sotsiaalse rehabilitatsiooni erasektorile üleandmisega kaasnenud muudatustest riigi vastutuse struktuuris. Tasu maksmise kohustuse ülevõtmise õiguslikust mudelist, sotsiaalse rehabilitatsiooni elementide vastavusest avaliku ülesande tunnustele ja sotsiaalse rehabilitatsiooniga seotud tagamiskohustusest

  15. Eesti Panga staatus ning selle muutumine seoses euro kasutuselevõtuga : [bakalaureusetöö] / Ursula Laido ; Tartu Ülikooli Õigusinstituut ; juhendaja: Madis Kallion

    Index Scriptorium Estoniae

    Laido, Ursula

    2005-01-01

    Eesti Panga ajalugu, õiguslik staatus, juhtimine, ülesanded, pädevus, Euroopa Liit kui Euroopa Majandus- ja Rahaliit, keskpankade süsteem, euro, eurole üleminek ja sellest tulenevad muudatused Eesti Panga jaoks

  16. Aritmeetika tekstiülesannete lahendamisoskusest / Anne Kana, Anu Palu

    Index Scriptorium Estoniae

    Kana, Anne

    2006-01-01

    Uurimusest selgus, millised aritmeetika teksiülesanded tekitavad õpilastele raskusi. Erinevate ülesannete tüüpide võrdlus ja analüüs aitaksid kaasa tekstiülesannete lahendamise oskuse kujundamisele

  17. Euroopa Liidu lävel / Tiiu Sizova

    Index Scriptorium Estoniae

    Sizova, Tiiu

    2003-01-01

    Ilmunud ka: Severnoje Poberezhje : Okruzhajushtshaja Sreda 6. dets. lk. 1. Eesti keskkonnahoiu seisukohalt on Ida-Virumaa üks problemaatilisemaid piirkondi, ülesanded seoses rahvusvaheliste direktiivide täitmisega

  18. Tulemuslikkuse ja organisatsioonikultuuri vahelised seosed Eesti keskkoolides / Anne Aidla

    Index Scriptorium Estoniae

    Aidla, Anne, 1974-

    2004-01-01

    Autor järeldab uuringu tulemustele tuginedes, et kõrge tulemuslikkusega koolides on töötajad ülesande orientatsiooni hinnangutes optimistlikumad, keskmise tulemuslikkuse puhul aga pessimistlikumad. Tabel

  19. Liam Young, soovtulevike vormija = Liam Young; shaper of preferable futures / Veronika Valk

    Index Scriptorium Estoniae

    Valk, Veronika

    2016-01-01

    Lühike ülevaade austraalia päritolu arhitekti Liam Youngi avatud loengusarja raames peetud loengust, milles ta toob välja arhitektide ja linnaplaneerijate uued ülesanded linnaruumi kujundamisel tulevikus uute tehnoloogiatega

  20. School shooting threats as a national phenomenon: comparison of police reports and psychiatric reports in Finland / Atte Oksanen, Riittakerttu Kaltiala-Heino, Emma Holkerie, Nina Lindberg

    Index Scriptorium Estoniae

    Oksanen, Atte

    2015-01-01

    Uuringust, milles analüüsiti ja võrreldi riskitegureid, mis viitavad 12-18 aastaste õpilaste kalduvusele sooritada tapmist. Uuringus tuginetakse Soome politsei ja psühhiaatrilistele raportitele. Erinevustest politsei ja psühhiaatrilistes raportites välja toodud riskiteguritest

  1. Noortest, tööst ja Ida-Virumaast / Aldis Alus

    Index Scriptorium Estoniae

    Alus, Aldis, 1967-

    2012-01-01

    Sisekaitseakadeemia politsei- ja piirivalvekolledži lõpetanu Julia Vesjolkina lõputööst: "Sisekaitseakadeemia lõpetanud kadettide valmisolek asuda tööle Ida-Virumaale" (2012); juhendaja Piret Teppan. Politsei- ja Piirivalveameti Ida prefekti selgitused prefektuuri personalipoliitika kohta

  2. Dali ärijuht võeti võltsijana vahi alla

    Index Scriptorium Estoniae

    2000-01-01

    Hispaania politsei vahistas Salvador Dali sekretäri ja ärijuhi Peter Moore'i, süüdistades teda Dali litograafiate võltsingute organiseeritud müügis. Moore'ile kuulunud laohoonest Cadaquesis leidis politsei 80 Dali litokivi.

  3. Eesti elanike turvatunne ja nende hinnang politseitööle aastal 2006 / Triin Rannama

    Index Scriptorium Estoniae

    Rannama, Triin

    2007-01-01

    Uuringu "Elanike hinnangud Eesti politseile 2006" tulemused näitavad, et hinnangud politsei tegevusele on paranenud, kuid elanike turvatunne on vähenenud. Lisa: Nii valmis uuring "Elanike hinnangud Eesti politseile 2006". Diagrammid: Hinnangud erinevatele politseiga seonduvatele omadustele 2006. aastal; Elanike turvatunne 2006. aastal; Elanike rahulolu politsei tööga regioonides; Rahulolu prefektuuriti

  4. Gümnaasiumi kodanikuõpetuse eksam / Mare Räis

    Index Scriptorium Estoniae

    Räis, Mare

    2002-01-01

    2001/2002. õppeaastal esimest korda toimuva kodanikuõpetuse eksami korraldusest. Eksamitöö koosneb kolmest osast: I osa - arutlus (20 p); II osa - töö allikatega: pildid, dokumendid, andmed, kaardid vms (30 p); III osa - ülesanded on paigutatud kahele A3 lehele, mis on kujundatud vihikuna, st ülesanded järgnevad üksteisele lehekülgede kaupa (50 p). Arutluse hindamisjuhend

  5. 3.5% urea-linked gelatin is as effective as 6% HES 200/0.5 for volume management in cardiac surgery patients

    NARCIS (Netherlands)

    van der Linden, Philippe J.; de Hert, Stefan G.; Daper, Anne; Trenchant, Anne; Schmartz, Denis; Defrance, Pierre; Kimbimbi, Pierre

    2004-01-01

    PURPOSE: To compare the efficacy of volume expansion with 3.5% gelatin and 6% hydroxyethyl starch 200/0.5 in patients undergoing cardiac surgery. The second objective was to compare the two colloids in terms of blood losses and allogeneic blood transfusion exposure rate. METHODS: In this open-label

  6. Näitlejad ühes mullis, esietendus ukse ees / Hendrik Toompere jr. jr., Liisa Pulk, Mait Joorits ... [jt.] ; intervjueerinud Liisi Seil ; kommenteerinud Helle Leppik

    Index Scriptorium Estoniae

    2010-01-01

    Vestlus Eesti muusika- ja teatriakadeemia lavakunstikooli XXIV lennu tudengitega, kelle lõputöö Tom Stoppardi näidend "Arkaadia" etendub Ugala laval. Lisatud Ugala teatri kirjandustoimetaja arvamus

  7. Transcriptional dynamics elicited by a short pulse of Notch activation involves feed-forward regulation by E(spl)/Hes genes

    Czech Academy of Sciences Publication Activity Database

    Housden, B. E.; Fu, A. G.; Krejčí, Alena; Bernard, F.; Fischer, B.; Tavaré, S.; Russell, S.; Bray, S. J.

    2013-01-01

    Roč. 9, č. 1 (2013), e1003162 ISSN 1553-7404 Grant - others:BBSRC project grant(GB) BB/F00897X/1; MRC programme(GB) G0800034 Institutional support: RVO:60077344 Keywords : split complex genes * mammalian neural development * T-cell leukemia Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 8.167, year: 2013 http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1003162

  8. A cost-benefit analysis of the immunisation of children against respiratory syncytial virus (RSV) using the English Hospital Episode Statistics (HES) data set.

    Science.gov (United States)

    Thomas, Gareth

    2018-03-01

    Respiratory syncytial virus (RSV) is a common cause of respiratory infection that is highly prevalent in infants, particularly those with underlying medical conditions. Severe cases of RSV require hospitalisation as well as admission to intensive care and may even result in death. The objective of the study was to measure the net benefits that could arise from an immunisation programme of infants that may well eradicate RSV to a high degree and save the direct and indirect medical care costs from hospitalisation, morbidity and the gain from potential life-time earnings by reducing the probability of mortality. In this context, the majority of existing empirical investigations are based on data from clinical trials, and where relevant facts are not available, a series of strong assumptions is derived from the published literature, whereas in this study, for the first time, the hospital episode statistics database is used to calculate the cost-benefit ratios. The methodology of the analysis adopts a cost-benefit approach to assess the impact of the immunisation and whether it is beneficial to society. The underlying assumptions of the basic model are assessed by adopting a sensitivity analysis. The results show that a number of categories are cost-effective with the use of the passive drug, which means benefits by raising the life expectancy and quality as well as reducing the resource burden on society.

  9. Notch 1 Receptor, Delta 1 Ligand and HES 1 Transcription Factor are Expressed in the Lining Epithelium of Periapical Cysts (Preliminary Study)

    OpenAIRE

    Meliou, E; Kerezoudis, NP; Tosios, KI; Kiaris, H

    2010-01-01

    Periapical cyst is a chronic inflammatory disorder of periradicular tissues. The precise pathological mechanisms involved in periapical cyst enlargement remain unclear. Notch signaling is an evolutionarily conserved pathway with a regulatory role in cell fate decisions during development and in carcinogenesis. To date, there are no published data available on the expression of Notch signaling components in periapical cysts or any other jaw cyst. In this immunohistochemical study we have exami...

  10. Haidary endised alluvad pääsesid trahviga / Mare Mere

    Index Scriptorium Estoniae

    Mere, Mare

    2004-01-01

    Tallinna linnakohtu otsuse kohaselt peavad siiani politsei poolt tabamata Afganistani päritolu piimaärimehe Mohammad Yaqub Haidary endised alluvad pankrotistunud Rapla Dairy'st tasuma karistuseks maksupettuse eest 7000 kuni 20 000 krooni trahvi

  11. Mezhpravitelstvennoje soglashenije mezhdu Estonijei i Latvijei / Kristina Kareva

    Index Scriptorium Estoniae

    Kareva, Kristina

    2006-01-01

    Siseminister Kalle Laanet viibis Leedus, et arutada Läti siseministri Dzintars Jaundzheikarsiga ja Leedu siseministri Gintaras Furmanaviciusega ettevalmistusi Schengeni õigusruumiga liitumiseks ning sõlmida Eesti ja Läti vaheline politsei koostöölepe

  12. Kuressaare politseijaoskond sõlmis tulemuslepingu

    Index Scriptorium Estoniae

    2004-01-01

    Prefekt Kalle Laanet ja ülemkomissar Ants Ley allkirjastasid tulemuslepingu, kus on sätestatud Kuressaare politseijaoskonna tegevus Eesti politsei ja Lääne politseiprefektuuri 2004. aastaks seatud eesmärkide saavutamiseks

  13. Alleged assassin "jumped of bridge" / Talis Saule Archdeacon

    Index Scriptorium Estoniae

    Archdeacon, Talis Saule

    2007-01-01

    Uued asjaolud ei lisa selgust julgeolekuteenistuse töötaja Edgars Gulbise sattumisse Daugava jõkke 26. septembril uurimisvanglasse konvoeerimise ajal. Kahtlustatakse, et politsei kasutas tema suhtes vägivalda

  14. Väljaöeldut tagasi võtta ei saa / Raivo Uukkivi

    Index Scriptorium Estoniae

    Uukkivi, Raivo, 1962-

    2007-01-01

    Õpetaja suhtumisest õpilasse. Õpetaja professionaalsusest ja tema missioonitundest. Vastukaja artiklile: Krull, Edgar. Kõvad seadused ja politsei ei vabasta kohustusest kasvatada // Õpetajate Leht (2007) 16. veebr., lk. 5. Jüri Gümnaasiumi koolikonfliktist

  15. Mundrimehe alla ajanud politseiniku varem joobnult tehtud avarii vaikiti maha / Oliver Rand

    Index Scriptorium Estoniae

    Rand, Oliver, 1971-

    2008-01-01

    Politsei vaneminspektor Kalle Kolk, kes sõitis Tartu abipolitseinikule otsa, tegi avarii ka 2003. aastal. Lõuna politseiprefekt Aivar Otsalt põhjendab Kalle Kolgile teenistusmärgi andmist 2007. aastal

  16. Koolis tulistamise järelmid: korralikud kriisikavad ja õpetajatele rohkem õigusi / Joosep Värk

    Index Scriptorium Estoniae

    Värk, Joosep

    2015-01-01

    Vestlusest Haridus- ja Teadusministeeriumi koolivõrgu osakonna konsultandi Jürgen Rakaselja, Politsei- ja Piirivalveameti arendusosakonna valmisoleku ja reageerimise büroo juhi Marti Magnuse ja Rocca al Mare Kooli direktori Kaido Kreintaaliga

  17. Uletutshiliss tonnõ tekstilja / Jevgeni Kapov

    Index Scriptorium Estoniae

    Kapov, Jevgeni

    2006-01-01

    Narva tekstiilivabrikus AS Polytex.ee.printing toodetud ja Prantsusmaale tellijale saadetud 18 tonni tekstiilitooteid kogumaksumusega 700 000 krooni ei jõudnud sihtpunkti, politsei ja firma teevad koostööd kadunud kauba ja kaubavedu korraldanud firma leidmiseks

  18. Napolis käib maffiasõda / Aadu Hiietamm

    Index Scriptorium Estoniae

    Hiietamm, Aadu, 1954-

    2006-01-01

    Itaalia linnas Napolis ja selle lähistel on viimasel ajal tapetud üle 70 inimese, politsei väitel on tegemist maffia liikmete omavaheliste arveteklaarimisega. Itaalia võimud saadavad Napolisse lisaks 1000 politseinikku

  19. Kalalaev Katrin jääbki kadunuks / Kadi Laid

    Index Scriptorium Estoniae

    Laid, Kadi, 1976-

    2003-01-01

    Politsei lõpetab kalalaeva Katrin huku uurimise, kuna vaatamata korduvatele sukeldumistele, ei ole suudetud laevavrakki leida. Laeva kindlustanud Bico-Leks Kindlustuse AS-i järglane ERGO Kindlustus peab omanikule kindlustusraha välja maksma

  20. Politseinike tegevust hinnates ükski ekspert külmaks ei jäänud / Kärt Anvelt, Raimo Poom

    Index Scriptorium Estoniae

    Anvelt, Kärt, 1973-

    2007-01-01

    Hinnangutest politsei tegevusele Tallinnas toimunud meeleavaldusel. Vt. samas: Ekspert: märuliöö võttis eestlastelt turvatunde; Öistes rahutustes peeti kinni ligi 500 jõhkrutsejat; Jaanus Piirsalu. Eesti saatkond Moskvas piirati puust taraga

  1. Politseiniku lustlik ABC / Pekka Erelt

    Index Scriptorium Estoniae

    Erelt, Pekka, 1965-

    1999-01-01

    Politsei on välja andnud 'Politseiniku ABC', mis antakse igale politseinikule. Karikaturist Heiki Ernits on peaaegu igale taskuraamatu leheküljele joonistanud pildikese mundrimeestest kentsakates situatsioonides.

  2. Arreteeriti Salvador Dali võltsija

    Index Scriptorium Estoniae

    1999-01-01

    Hispaania politsei arreteeris Cadaquesis Salvador Dali endise sekretäri, ameeriklase John Peter Moore'i, kes tabati Dali võltslitograafiate müümiselt. Läbiotsimisel leiti Moore'i majast sadu võltsinguid

  3. Narva ametnikku kahtlustatakse pistise nõudmises / Tanel Mazur, Aleksei Gorpintshenko

    Index Scriptorium Estoniae

    Mazur, Tanel, 1971-

    2008-01-01

    Autorite väitel võib Narva linnavalitsuse linnavara- ja majandusameti direktori kohusetäitja Arkadi Nikolajevi vahistamine olla seotud laiema korruptsiooniskeemiga. Skeem: Politsei uurib Arkadi Nikolajevi tegemisi

  4. Mõrvarid jahtisid Riigi Kinnisvara juhti / Andris Tammela

    Index Scriptorium Estoniae

    Tammela, Andris

    2006-01-01

    Politsei pidas kinni kolm meest, keda kahtlustatakse oktoobri alguses Tallinna koolitüdruku Veronika Dari tapmises, väidetavalt oli meeste tegelik sihtmärk Riigi Kinnisvara aktsiaseltsi juht Tiit Ottis

  5. Teemandiärimees nõuab Eestilt 25 miljonit krooni / Rasmus Kagge

    Index Scriptorium Estoniae

    Kagge, Rasmus, 1977-

    2004-01-01

    Iisraeli kodanik Uri Marty esitas Tallinna linnakohtule kahjunõude, seses politsei poolt 2001. aastal aluseltult vahi all hoidmisega, mille tõttu ta olevat kaotanud suurt kasumit tõotanud teemandiäri

  6. Menschenrechtliche Grenzen des polizeilichen Unterbindungsgewahrsams / Joachim Renzikowski, Reimund Schmidt-De Caluwe

    Index Scriptorium Estoniae

    Renzikowski, Joachim

    2013-01-01

    Politsei poolt vahi all pidamise inimõiguslikest piiridest. Vastavast kohtulahendist (Schwabe und M. G. gegen Deutschland), mis puudutab inimõiguste ja põhivabaduste kaitse konventsiooni art. 5 lk. 1 ja art. 11

  7. Õigusrikkumised [1997] = Violation of jurisdiction

    Index Scriptorium Estoniae

    1998-01-01

    Politseis registreeritud kuriteod, 1997, kuritegevuse tase 10 000 elaniku kohta 1993-1997, kuriteod liikide lõikes, 1997, väljaselgitatud kuritegudes süüdistatavad isikud Tallinnas, 1997, kuritegevus ja selle avastamine Tallinna asumites

  8. Judgment of the Supreme Court en banc on behalf of the Republic of Estonia no. of the case 3-3-1-44-11 : date of judgment 3 July 2012

    Index Scriptorium Estoniae

    2014-01-01

    Kohtulahendi 3-3-1-44-11 (Politsei- ja Piirivalveameti kodakondsus- ja migratsiooniosakonna kassatsioonkaebus Tallinna Ringkonnakohtu 10. veebruari 2011. a otsuse peale haldusasjas nr 3-10-1379 Petr Dmitruki ja Nadežda Dmitruki kaebustes Politsei- ja Piirivalveameti 23. märtsi 2010. a otsuste nr 15.3-04/158 ja 15.3-04/159 tühistamiseks) tekst inglise keeles

  9. Early resuscitation with polymerized bovine hemoglobin reverses acidosis, but not peripheral tissue oxygenation, in a severe hamster shock model.

    Science.gov (United States)

    Wettstein, Reto; Tsai, Amy G; Harder, Yves; Erni, Dominique; Intaglietta, Marcos

    2006-11-01

    Awake hamsters equipped with the dorsal window chamber preparation were subjected to hemorrhage of 50% of the estimated blood volume. Initial resuscitation (25% of estimated blood volume) with polymerized bovine hemoglobin (PBH) or 10% hydroxyethyl starch (HES) occurred in concert with an equivolumetric bleeding to simulate the early, prehospital setting (exchange transfusion). Resuscitation (25% of estimated blood volume) without bleeding was performed with PBH, HES, or autologous red blood cells (HES-RBCs). Peripheral microcirculation, tissue oxygenation, and systemic hemodynamic and blood gas parameters were assessed. After exchange transfusion, base deficit was -8.6 +/- 3.7 mmol/L (PBH) and -5.1 +/- 5.3 mmol/L (HES) (not significant). Functional capillary density was 17% +/- 6% of baseline (PBH) and 31% +/- 11% (HES) (P < 0.05) and arteriolar diameter 73% +/- 3% of baseline (PBH) and 90% + 5% (HES) (P < 0.01). At the end, hemoglobin levels were 3.7 +/- 0.3 g/dL with HES, 8.2 +/- 0.6 g/dL with PBH, and 10.4 +/- 0.8 g/dL with HES-RBCs (P < 0.01 HES vs. PBH and HES-RBCs, P < 0.05 PBH vs. HES-RBCs). Base excess was restored to baseline with PBH and HES-RBCs, but not with HES (P < 0.05). Functional capillary density was 46% +/- 5% of baseline (PBH), 62% + 20% (HES-RBCs), and 36% +/- 19% (HES) (P < 0.01 HES-RBCs vs. HES). Peripheral oxygen delivery and consumption was highest with HES-RBCs, followed by PBH (P < 0.05 HES-RBCs vs. PBH, P < 0.01 HES-RBCs and PBH vs. HES). In conclusion, the PBH led to a correction of base deficit comparable to blood transfusion. However, oxygenation of the peripheral tissue was inferior with PBH. This was attributed to its negative impact on the peripheral microcirculation caused by arteriolar vasoconstriction.

  10. AGG Maailmameistrivõistluste eel

    Index Scriptorium Estoniae

    2008-01-01

    12.-14. juunini toimuvad Torontos Aesthetic Group Gymnastics (AGG) maailmameistrivõistlused Toronto Ülikooli võimlemiskeskuses. Nende võistluste korraldamise raske ülesande on võtnud oma õlule Kanada eestlaste võimlemisklubid Kalev Estienne ja Ritmika vastavalt Evelyn Koobi ja Annely Riga juhtimisel

  11. Forum Academicum in itinere : [juriidiliste formularide kogumik] / tõlkinud Nele Siimre ... [jt.] ; Forum Academicum Inter Nationes ; koost. Andrus Miilaste ; toim. Virve Tamm, Andrus Miilaste ; eess.: Peter-Friedrich Krienitz

    Index Scriptorium Estoniae

    1996-01-01

    Sisaldab ka: Õigusriigi ülesanded : säilitada ja muuta / Ingo von Münch, tlk. Mari Tarvas, Andrus Miilaste ; Inglismaa konstitutsioonist. Seadusandluse vaimust / Charles Montesquieu, tlk. Kattri Türk ; Loodusseadus. Seadustest / Cicero, tlk. Kattri Türk

  12. From national banking supervision to a centralized model of prudential supervision in Europe? : the stability function of the Single Supervisory Mechanism / Gianni Lo Schiavo

    Index Scriptorium Estoniae

    Schiavo, Gianni Lo

    2014-01-01

    Euroala pankade ühtse pangandusjärelevalve mehhanismist (SSM). Euroopa Nõukogu määrusest 1024/2013, millega antakse Euroopa Keskpangale eriülesanded seoses krediidiasutuste usaldatavusnõuete täitmise järelevalve poliitikaga

  13. Eesti Energia salajased uuringud / Sulev Vedler, Tarmo Vahter

    Index Scriptorium Estoniae

    Vedler, Sulev, 1970-

    2007-01-01

    Ilmunud ka: Infopress 2. märts nr. 9 lk. 160. Hoitakse salajas Briti konsultatsioonifirma Lexicon Partners poolt koostatud uuringut, mida peaks Eesti riik peale hakkama oma osalusega monopoolses elektriettevõttes Eesti Energia. Majandusminister Edgar Savisaar lasi uuringu ministeeriumisse tuua ning andis juristidele ülesande analüüsida, kui palju saab dokumendist avaldada. Vt. samas: Elektri hind

  14. Raad ja tema koda : Tallinna linna valitsemise ajaloost = Magistracy and its Stronghold : on the History of the Governance of the City of Tallinn / Tiina Kala

    Index Scriptorium Estoniae

    Kala, Tiina

    2004-01-01

    Tallinna rae kujunemine, koosseis, ülesanded, asjaajamine ning kohtukorraldus. Tallinna õiguslik seisund Rootsi ja Vene ajal. Eestlaste mõju suurenemine Tallinna linnaomavalitsuses 19. ja 20. sajandi vahetusel. Enamlaste ja Saksa okupatsiooni aegne linnavõim. Tallinna Linnavalitsus Eesti Vabariigis 1919-1940. Nõukogude ja Saksa okupatsiooni aegne linnavõim alates 1940. aastast

  15. Eesti projekteerijad pürivad Euroopasse

    Index Scriptorium Estoniae

    2005-01-01

    Eesti Projektbüroode Liit korraldas oktoobris 2005 juhatuse liikmetele töövisiidi Brüsselisse, leidmaks võimalusi muuta Euroopa Liidu projektide lähteülesanded ja tingimused Eesti firmadele soodsamaks. Külastati inseneri- ja arhitektuuribüroosid SNC Lavalin Europe ning Samyn & Partners

  16. Miks meile vaja maastikuarhitektuuri? = Why do we need landscape architecture? / Reinhard Micheller, Günther Schalk, Katrin Koov

    Index Scriptorium Estoniae

    Micheller, Reinhard, 1977-

    2013-01-01

    EKA arhitektuuri ja linnaplaneerimise osakonna III kursuse 2009-2012 aastail erialase projekteerimise ülesande raames on vestlusringis maastikuarhitektuurse osa juhendajad Reinhard Micheller ja Günther Schalk (michellerundschalk, München) ning Katrin Koov (KAVAKAVA, EKA maastikuarhitektuuri õppekava juht)

  17. Sadamaala - linnauuenduse võtmekoht = The Port District - Key Location for Urban Renewal / Ando Keskküla ; interv. Triin Ojari

    Index Scriptorium Estoniae

    Keskküla, Ando

    2006-01-01

    Intervjuu omaaegse Tallinna linnavolikogu liikme, sadamaala tsoneerimise lähteülesande komisjoni esimehe Ando Keskkülaga, kes Eesti Kunstiakadeemia rektorina kavandas akadeemia kolimist endisesse Patarei vanglasse. Sadamaala kui koht uuetüübilise kultuuritööstuse paiknemiseks

  18. Vana rasv on otsas / Imre Siil

    Index Scriptorium Estoniae

    Siil, Imre

    2005-01-01

    Riigikantselei ja Eesti Informaatikakeskuse endine juht põhjustest, miks e-riik on jõudnud praegusesse olukorda. Tema sõnul on arvestatav baas e-riigi edasiseks arenguks olemas, kuid ülesanded lähevad keerulisemaks ja vaja oleks uusi ideid ning arengustrateegiaid

  19. Bill Barbour : mõned ideed kasvavatele firmadele / Bill Barbour ; interv. Taivo Paju

    Index Scriptorium Estoniae

    Barbour, Bill

    2005-01-01

    Suurbritannia üks kogenumaid ärikonsultante firmade ärikasvumudelist, mis aitab ettevõttel järgmisesse etappi üle minna. Mida peaks ettevõtja enne äri alustamist läbi mõtlema ning millised eesmärgid ja ülesanded enda jaoks paika panema. Lisad: Bill Barbour; Universaalne kriisimudel

  20. Sündikaatlaenutehingu eripära ja õiguslikud probleemid / Natalja Sidorenko, Rain Raa

    Index Scriptorium Estoniae

    Sidorenko, Natalja

    2009-01-01

    Sündikaatlaenutehingu mõiste, põhimõtted, osapoolte ülesanded ja vastutus. Ülevaade rahvusvahelise sündikaatlaenuturu korraldamiseks asutatud organisatsioonist Loan Market Association ning selle poolt välja töötatud normdokumentidest

  1. Millist rahastamist vajab kodanikuühiskonna arendamine? / Urmo Kübar, Agu Laius

    Index Scriptorium Estoniae

    Kübar, Urmo, 1978-

    2007-01-01

    Rahastamine on avaliku võimu ja kodanikeühenduste koostöö vorm, mida tuleks käsitada vastastikuste huvide elluviimisena. Riigi, erasektori ja omatulude osa rahastamises, teiste riikide kogemusi. Kodanikuühiskonna sihtkapitali (KÜSK) eesmärgid ja ülesanded. Diagramm: Vabaühenduste sissetulekuallikad

  2. Arhiivi optiline alateadvus : kunstniku töö ja kuraatoritöö / Hanno Soans

    Index Scriptorium Estoniae

    Soans, Hanno, 1974-

    2000-01-01

    Moderna Museetis eksponeeritud šoti kunstniku Douglas Gordoni filmiinstallatsioonist, kus kuraatori ülesande võtnud kunstnik esitas läbilõike arhiivist ja visualiseeris ühe võimaluse selle lugemiseks. Eesti Kunstimuuseumis Eha Komissarovi kureeritud näitusest 'XX sajandi kunstniku mälestuseks' (kujundaja Jaan Toomik), mille esmane ülesanne oli arhiivi elustamine

  3. Relationship between organisational culture and individual values of the Russian-speaking members of an organisation in Lithuania / Elina Tolmats

    Index Scriptorium Estoniae

    Tolmats, Elina

    2003-01-01

    Uurimuse tulemused näitasid, et madalamaid hinnanguid organisatsioonikultuuri ülesande orientatsioonile annavad organisatsiooni need liikmed, kes väärtustavad kõrgelt ümbritsevat ilu, kõrgemaid hinnanguid aga need, kelle jaoks on oluline tugiväärtus edasipüüdlik. Tabelid

  4. Response of Complex Undefined Hypereosinophilic Syndrome to Treatment with Imatinib.

    Science.gov (United States)

    Yılmaz, İnsu; Kaynar, Leylagül; Tutar, Nuri; Pala, Çiğdem; Canöz, Özlem; Yıldırım, Afra; Büyükoğlan, Hakan; Gülmez, İnci

    2016-07-01

    Hypereosinophilic syndomes (HESs) include potentially lethal multisystem disorders characterized by eosinophilic infiltration of a variable spectrum of target organs, predominantly the skin, heart, lungs, gastrointestinal tract, and nervous system. Based on recent advances in molecular and genetic diagnostic techniques and increasing experience with differences in clinical features and prognosis, subtypes have been defined, including "myeloproliferative-HES ", "lymphocytic-HES", "familial eosinophilia", "overlap HES", "undefined HES" ("complex undefined HES", "simple undefined HES", "episodic undefined HES") and "eosinophil associated diseases" (such as Churg-Strauss syndrome). HES should be kept in mind in the differential diagnosis of eosinophilic lung diseases especially in patients with peripheral eosinophilia and pulmonary infiltrates. Corticosteroids represent an effective firstline approach to decreasing eosinophil counts in the majority of cases. Imatinib might be used for corticosteroid nonresponders. We herein report a patient with "complex undefined HES" who had disease resistant to corticosteroids, but who had a significant response after treatment with imatinib.

  5. Hybrid entanglement concentration assisted with single coherent state

    International Nuclear Information System (INIS)

    Guo Rui; Zhou Lan; Sheng Yu-Bo; Gu Shi-Pu; Wang Xing-Fu

    2016-01-01

    Hybrid entangled state (HES) is a new type of entanglement, which combines the advantages of an entangled polarization state and an entangled coherent state. HES is widely discussed in the applications of quantum communication and computation. In this paper, we propose three entanglement concentration protocols (ECPs) for Bell-type HES, W-type HES, and cluster-type HES, respectively. After performing these ECPs, we can obtain the maximally entangled HES with some success probability. All the ECPs exploit the single coherent state to complete the concentration. These protocols are based on the linear optics, which are feasible in future experiments. (paper)

  6. Hydroxyethyl starch in sepsis

    DEFF Research Database (Denmark)

    Haase, Nicolai Rosenkrantz Segelcke

    2014-01-01

    BACKGROUND: Hydroxyethyl starch (HES) is a colloid that has been widely used for fluid resuscitation for decades. The newest generation of HES, tetrastarch, was believed to provide an efficient volume expansion without causing the side effects observed with former HES solutions. However, this bel......BACKGROUND: Hydroxyethyl starch (HES) is a colloid that has been widely used for fluid resuscitation for decades. The newest generation of HES, tetrastarch, was believed to provide an efficient volume expansion without causing the side effects observed with former HES solutions. However...... types of patients is unclear, but so far no group of patients with an overall benefit of HES beyond surrogate markers has been identified. In line with this, the European Medicines Agency's Pharmacovigilance Risk Assessment Committee now recommends that the marketing authorisations of all HES solutions...

  7. Cloning, characterization and promoter analysis of common carp

    Indian Academy of Sciences (India)

    Some members of hairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes, the her6 gene, a member of the HES family, is the homologue of hes1 in mammals and chicken. In this study, the her6 gene ...

  8. Hydroxyethyl starch for resuscitation

    DEFF Research Database (Denmark)

    Haase, Nicolai; Perner, Anders

    2013-01-01

    PURPOSE OF REVIEW: Resuscitation with hydroxyethyl starch (HES) is controversial. In this review, we will present the current evidence for the use of HES solutions including data from recent high-quality randomized clinical trials. RECENT FINDINGS: Meta-analyses of HES vs. control fluids show clear...

  9. Raman-based detection of hydroxyethyl starch in kidney allograft biopsies as a potential marker of allograft quality in kidney transplant recipients

    Science.gov (United States)

    Vuiblet, Vincent; Fere, Michael; Bankole, Ezechiel; Wynckel, Alain; Gobinet, Cyril; Birembaut, Philippe; Piot, Olivier; Rieu, Philippe

    2016-09-01

    In brain-dead donor resuscitation, hydroxyethyl starch (HES) use has been associated with presence of osmotic-nephrosis-like lesions in kidney transplant recipients. Our aim was to determine whether the presence of HES in protocol renal graft biopsies at three months (M3) after transplantation is associated with renal graft quality. According to the HES administered to the donor during the procurement procedure, two groups of patients were defined according graft exposition to HES: HES group, (N = 20) and control group (N = 6). Detection and relative quantification of HES was performed by Raman spectroscopy microimaging on M3 protocol renal graft biopsies. Statistical analyses were used to investigate the association between Raman data and graft characteristics. HES spectral signal was revealed negative in the control group, whereas it was positive in 40% of biopsies from the HES group. In the HES group, a stronger HES signal was associated with a lower risk of graft failure measured by the Kidney Donor Risk Index (KDRI) and was correlated with the allograft kidney function. Thus, HES accumulation in donor kidney, as probed by Raman biophotonic technique, is correlated with the quality of donor kidney and consequently the graft renal function and graft survival.

  10. Iraagi hall kardinal usub uude riiki / Muhsen Naif Al-Faisal Al-Jarba ; interv. Ivar Soopan

    Index Scriptorium Estoniae

    Al-Jarba, Muhsen Naif Al-Faisal

    2004-01-01

    Iraagi presidendi ja peaministri nõuniku sõnul tahab Iraak üles ehitada tõelise armee, politsei, piirivalve. Pärast seda koalitsiooniarmeed enam ei vajata. Rahu kogu Lähis-Idas sõltub Iisraelist, ütleb nõunik. Vt. samas: Kuidas ma tutvusin sheigiga

  11. Vale-Cesarid turul / Maria-Kristiina Soomre

    Index Scriptorium Estoniae

    Soomre, Maria-Kristiina, 1978-

    2002-01-01

    Prantsuse politsei on kinni pidanud jõugu, keda kahtlustatakse Prantsuse ja Belgia kunstituru üleujutamises Césari (1921-1998) känkrametalltööde koopiatega. Pariisi galeriidest on konfiskeeritud üle 200 teose. Césari tööde signatuuriks on tema näpujälg

  12. Mida pakub Delta? / Teele Kurm

    Index Scriptorium Estoniae

    Kurm, Teele

    2011-01-01

    Politsei- ja Piirivalveamet võtab kasutusele ühise Siseministeeriumi infotehnoloogia- ja arenduskeskuse ning Webmedia AS koostööna loodud dokumendihaldussüsteemi Delta. Kust sai Delta oma nime? Projekti "Dokumendihaldussüsteemi juurutamine Siseministeeriumi haldusalas" eesmärgid

  13. Eesti munitsipaalpolitsei Lääne taustal / Allan Alaküla

    Index Scriptorium Estoniae

    Alaküla, Allan, 1968-

    2002-01-01

    Ilmunud ka: Stolitsa 5. dets. lk. 1. Riigikogu liikmete ja Euroopa politsei- ja omavalitsustegelaste arvamused munitsipaalpolitseist: Jaanus Männik, Keith Whitmore, Peter Shorer, Mai Treial, Michel Albin, Jaana Padrik, Jean Louis Renier, Eva Dohnalova, Calin Catalin Chirita, Aleksei Aleksandrov, Mohammad Nazir, Arvo Sirendi. Parlamendisaadik

  14. Prefekt võiks olla tsivilist / Sven Kesler

    Index Scriptorium Estoniae

    Kesler, Sven

    2003-01-01

    Saku vallavanema Sven Kesleri sõnul puudub politseinike igapäevatöös mõtestatud strateegiliselt planeeritud tegevus ning ta toob selle kohta näiteid. Kavandatavat politsei reformikava pooldades leiab ta, et prefekti ametisse võiks nimetada tsiviilisiku, sest politseitõõ operatiivjuhtimine ning organisatsiooni juhtimine on täiesti erinevad juhtimisvaldkonnad

  15. Ametiühing nõuab riigitöötajatele palgalisa

    Index Scriptorium Estoniae

    2007-01-01

    EAKL-i delegatsioon taotleb algavatel läbirääkimistel valitsusdelegatsioonilt riigiteenistujatele 15%-list palgatõusu, politsei-, pääste- ja vanglatöötajatele alampalga tõstmist 9000 kroonile. EAKL-i esimehe Harri Taliga arvamus

  16. Christie's politseiga pahuksis / Maria-Kristiina Soomre

    Index Scriptorium Estoniae

    Soomre, Maria-Kristiina, 1978-

    2001-01-01

    9. II Londonis toimuv Christie'si kunstioksjon on saanud politsei teravdatud tähelepanu osaliseks kolme Andres Serrano foto pärast seeriast "History of Sex" (1996). On võimalik teoste müügilt kõrvaldamine.

  17. Terroristijaht keskendub pakistanlastele / Heiki Suurkask

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2005-01-01

    Egiptuse politsei otsib kuut pakistanlast, keda kahtlustatakse Sharm el-Sheikis toimunud terrorirünnakute toimepanekus. Pakistan peab Egiptuse võimude kahtlustusi alusetuks, riigi presidendi väitel al-Qaidat enam Pakistanis ei eksisteeri. Kaart, graafik: Egiptuse turism

  18. Saalivalvurid varastavad ka Barcelonas

    Index Scriptorium Estoniae

    2000-01-01

    Hispaania politsei tabas Barcelona Arheoloogiamuuseumi saalivalvuri Manuel Gasca, kes varastas muuseumi hoidlaist umbes 35 miljoni Eesti krooni ulatuses vana-egiptuse, foiniikia, etruski ja vana-rooma arheoloogilisi leide (mündid, keraamika, skulptuur jt.) ning itaalia graafiku Giambattista Piranesi 150 gravüüri.

  19. Kalle Klandorf: politseinikud tuleb taas tuua tänavale : Tallinna abilinnapea leiab, et munitsipaalpolitsei vajab seadusega antud suuremaid volitusi / interv. Andrus Allika

    Index Scriptorium Estoniae

    Klandorf, Kalle, 1956-

    2011-01-01

    Kalle Klandorf leiab, et kuritegevuse vähenemisega, nagu väidab statistika, pole Eestis küll põhjust hoobelda. Kuritegusid registreeritakse lihtsalt vähem – inimesed ei tule politsei juurde, nad löövad käega

  20. Kolmteist piirivalvurit käisid ohutut jalgrattasõitu propageerimas / Velve Põldoja, Georgi Skorobogatov

    Index Scriptorium Estoniae

    Põldoja, Velve

    2009-01-01

    17.-18. juuli 2009 toimunud Eesti Posti, Eesti Politsei, Eesti Piirivalve, Falck Autoabi, Coca-Cola Hellenic Eesti ja Regio ühiselt korraldatud rattaretkest "Tahan sõita ohutult!", kus rahvale tutvustati rattaga ohutult liiklemise olulisust ja liikluses toimetulekut

  1. Kurjategijaist kunstnikud / Maria-Kristiina Soomre

    Index Scriptorium Estoniae

    Soomre, Maria-Kristiina, 1978-

    2000-01-01

    Oktoobris arreteeris New Yorgi politsei SOHO galeriis oma näitust üles pannud kunstnikud Todd James'i (29 a.) ja Josh Lazcano (25 a.), kes viljelevad graffitimõjutustega kunsti. Põhjuseks oli graffiti - kunstnikke süüdistatakse omandi kahjustamises

  2. Despite reprimand, minister pardons police chief / Ksenia Repson

    Index Scriptorium Estoniae

    Repson, Ksenia

    2005-01-01

    Siseminister Kalle Laanet möönis, et Politseiameti peadirektor Robert Antropov ei ole oma vanemaid politseisõidukiga Saaremaale toimetades reegleid rikkunud ning jättis tema lahkumispalve rahuldamata. Siiski karistas minister politseijuhti pooleaastase lisatasu kaotuse ja puhkuse tühistamisega

  3. В Сирии арестовали главаря банды / Аннели Аммас

    Index Scriptorium Estoniae

    Аммас, Аннели, 1962-

    2011-01-01

    Süüria ja Liibanoni politsei koostöös on kinni võetud Eesti jalgratturite röövimise ja pantvangistamise peaorganisaator Wael Abbas, kuid ametlikku kinnitust röövi peaorganisaatori kohta pole veel tulnud

  4. Süürlased tabasid Eesti ratturite röövi arvatava peakorraldaja / Anneli Ammas

    Index Scriptorium Estoniae

    Ammas, Anneli, 1962-

    2011-01-01

    Süüria ja Liibanoni politsei koostöös on kinni võetud selle aasta kevadise Eesti jalgratturite röövimise ja pantvangistamise peaorganisaator Wael Abbas, kuid ametlikku kinnitust röövi peaorganisaatori kohta pole veel tulnud

  5. Kadriorus pidutsenud noored said trahvi

    Index Scriptorium Estoniae

    2006-01-01

    Noortepeod Kadrioru presidendilossis. Politsei karistas Kadrioru lossis pidutsenud 17 noorukit suitsetamise ja alkoholi tarbimise eest trahviga. Ida politseiosakonna ülemkomissari Margus Kotter'i kommentaar. Ilmunud ka Eesti Päevaleht, 2006/Mar/4, lk. 5 ; Meie Maa, 2006/Mar/4, lk. 1 ; Järva Teataja, 2006/Mar/4, lk. 1 ; Põhjarannik, 2006/Mar/4, lk. 3

  6. Praxis and the disruption of organized crime groups / Stuart Kirby, Nicki Snow

    Index Scriptorium Estoniae

    Kirby, Stuart

    2016-01-01

    Kuigi kogu arenenud maailmas on uuringute alusel täheldatud kuritegevuse langust ei ole sellist tendentsi märgata organiseeritud kuritegevuse vallas. Antud uuring käsitleb inglise politsei poolt uuritud 15 organiseeritud kuritegelikku rühmitust

  7. Полиция опасается воров в своем доме

    Index Scriptorium Estoniae

    2008-01-01

    President Toomas Hendrik Ilves külastas Jõhvi politseiosakonda. Presidendis ja tema kaaskonnas tekitas imestust, et politsei üldkasutatav printer oli peidetud lukustatud metallvõre taha. President avaldas lootust, et praegused viletsad töötingimused asenduvad peagi 21. sajandi väärilistega

  8. Eestist saab politseiriik / Roy Strider

    Index Scriptorium Estoniae

    Strider, Roy, 1974-

    2007-01-01

    Autori väitel ei ole politsei pärast kavandatavat avaliku koosoleku seaduse muutmist enam kohustatud inimestele teatama, kui nende eraelu puutumatust on jälitustoiminguga riivatud. Autori sõnul tähendab see, et Eestist saab politseiriik

  9. Rahapesu andmebüroo uurib Ühispanga juhtumit / Inno Tähismaa

    Index Scriptorium Estoniae

    Tähismaa, Inno, 1970-

    2004-01-01

    Politsei rahapesu andmebüroo juhi Arnold Tenusaare kinnitusel alustatakse Ühispanga asepresidendi Lembit Kitteriga seotud off-shore firma Patent Developments Ltd. juhtumi uurimist, kus 1997. aastal firma pangaarvelt sularaha väljavõtmisel võis olla tegemist allkirja võltsimise ja rahapesuga

  10. Eel-Ernal võidutses sõjakool / Kristo Sinivee

    Index Scriptorium Estoniae

    Sinivee, Kristo

    2011-01-01

    Eel-Erna võistlus toimus 6.-7. mail Pärnumaal, sellel osales 21 võistkonda Kaitseliidust, kaitseväest, Kaitseväe Ühendatud Õppeasutustest, Politsei- ja Piirivalveametist ja vanglate relvastatud üksusest. Eel-Erna tulemused

  11. Ahmed Zakajev: Litvinenko tapeti, sest ta abistas omakasupüüdmatult tšetšeene / Tõnis Erilaid

    Index Scriptorium Estoniae

    Erilaid, Tõnis, 1943-

    2007-01-01

    Vene ajalehes Kommersant ilmus intervjuu endise Itškeeria ministri Ahmed Zakajeviga, kes ei kahtle, et Aleksandr Litvinenko tapmisega on seotud Andrei Lugovoi. Suurbritannia politsei tegutseb täie tõsidusega, et sundida Venemaad Andrei Lugovoid välja andma

  12. The police hack back: legitimacy, necessity and privacy implications of the next step in fighting cybercrime / R.L.D. Pool, B.H.M. Custers

    Index Scriptorium Estoniae

    Pool, R.L.D.

    2017-01-01

    Politsei võimaluste suurendamisest võitluses küberkuritegevusega. Saksamaa seaduseelnõust, mis võimaldab uurida kuritegevust internetis, eelnõu taustast ja sellest tulenevast võimalikust elanike privaatsuse ja teiste põhiõiguste riivest

  13. Viimase hetke kokkulepe päästis WTO tippkohtumise / Heiki Suurkask

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2005-01-01

    Kuigi EL-i eelarvekõnelustel Brüsselis jäeti ühine põllumajanduspoliitika puutumata, sunnib WTO tippkohtumisel Hongkongis saavutatud lepe 2013. aastast alates EL-i põllumajanduse toetamist revideerima. Lisa: Politsei vahistas 900 meeleavaldajat

  14. Suure kütusevarguse uurimise ajal kaduma läinud asitõendid on leitud / Irina Kiviselg

    Index Scriptorium Estoniae

    Kiviselg, Irina, 1961-

    2005-01-01

    Paar päeva pärast suure kütusevarguse avastamist Soldina jaamas kadus valvatavalt alalt osa asitõendeid. Politsei avastas Soldinast varastatud 8 tonni kütust selles kuriteos kahtlustatava kodu juures maasse kaevatud mahutist, leiti ka kuriteopaigalt varastatud pump

  15. Interesting coin finds of 2006 / Mauri Kiudsoo

    Index Scriptorium Estoniae

    Kiudsoo, Mauri, 1973-

    2007-01-01

    2006. aasta huvitavamatest mündileidudest. Lisaks arheoloogilistelt kaevamistelt saadud vermingutele omandati 2006. aastal kolm mündiaaret : Alansi aare Harjumaalt, ligi poolsada araabia hõbemünti sisaldav aare Tartust ning tänu politsei kaasabile jõudsid Eestisse tagasi Ubina hõbeaadre kaevamistelt varastatud hõbemündid. Eestist leitud rooma müntidest

  16. Tallinna info- ja abitelefon toetab ka hättasattunud lapsi / Hille Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Hille, 1941-2014

    2006-01-01

    Vt. ka Postimees : na russkom jazõke 16. jaan., lk. 5. Infotelefoni projektijuhi Herve Merivaldi sõnul ei sõanda lapsed lastekaitsetöötaja või politsei poole pöörduda. Abitelefonile helistades aitavad dispetsherid neil ühendust saada inimestega, kes neid aidata võivad

  17. Propaganda i kontrpropaganda / Aleksandr Tshaplõgin

    Index Scriptorium Estoniae

    Tšaplõgin, Aleksandr, 1964-

    2007-01-01

    Europarlamendis esines Öise Vahtkonna liige Juri Zhuravljov informatsiooniga väidetavast politsei vägivallast pronksööl. Riigikogu eurokomisjoni esimees Marko Mihkelson on väitnud, et ürituse organisaatoriks on Läti eurosaadik Tatjana Zhdanok. Europarlamendi liikme Katrin Saksa väiteid

  18. Eesti allilm suudab tõkestada GSM-levi / Rasmus Kagge

    Index Scriptorium Estoniae

    Kagge, Rasmus, 1977-

    2006-01-01

    Ilmunud ka: Postimees : na russkom jazõke 10. mai lk. 2. Politsei andmetel on Eesti kuritegelike jõukude käes praegu vähemalt paar GSM-levi segajat ehk jammerit. Politseil on õnnestunud konfiskeerida autovarastelt üks GSM-levi tõkestaja. Lisa. Kasutajapiirang. Vt. samas: Jammereid saab osta internetist

  19. The Supreme Court of Estonia constitutional judgement 3-3-1-35-10: judgment of the Supreme Court en banc : date of decision 31 August 2011

    Index Scriptorium Estoniae

    2013-01-01

    Kohtulahendi 3-3-1-35-10 (Riigiprokuratuuri ning Politsei- ja Piirivalveameti kassatsioonkaebused Tallinna Ringkonnakohtu 16. veebruari 2010. a otsuse peale haldusasjas nr 3-08-265 Ülar Kaasi (Kaas) kaebuses Eesti Vabariigi tekitatud 254 087 krooni suuruse kahju hüvitamise nõudes) tekst inglise keeles

  20. Tammetõru Kool 2001/2002 / Urmas Tokko

    Index Scriptorium Estoniae

    Tokko, Urmas

    2001-01-01

    Tartu Tamme Gümnaasium juures tegutsev Tammetõru Kool alustab viiendat õppeaastat. Tammetõru Kool on TTG juures tegutsev kaugõppe vorm põhikooliõpilastele ja mõeldud loodusteadustest huvitatud 8.-9. klasside õpilastele. Õppetöö toimub posti- või arvutiside teel. I vooru ülesanded bioloogias, füüsikas ja geograafias

  1. Kirjutamisest ja kirjutamise õpetamisest / Anne Uusen

    Index Scriptorium Estoniae

    Uusen, Anne

    2001-01-01

    Kirjutamise olemusest, kirjutamise motiveerimisest, kirjutamise (õpetamise ja õppimise) eesmärgid ja ülesanded. 1998/2000 õa. jooksul täienduskoolituses ja kaugõppes õppivate algklassiõpetajate hulgas läbi viidud küsitlusest, eesmärgiga uurida, mida õpetajad kirjutamise juures tähtsaks peavad, milliseid kirjutamisülesandeid nad õpilastele annavad ning milline on õpetajate enesehinnang oma koolitusele ja teadmistele kirjutamisest

  2. Linnadisaini lõpp(eesmärgid) / Michael Sorokin

    Index Scriptorium Estoniae

    Sorokin, Michael

    2008-01-01

    Autori arvates on linnadisain (urban design) jõudnud tupikusse. Ta on järjest vähem võimeline leidma leidlikke lahendusi linnade ja linnakodanike morfoloogilistele, funktsionaalsetele ja inimlikele vajadustele. Samal ajal kui ülesanded muutuvad keerukamaks ja pakilisemaks, on eriala peavoolustunud, muutudes potensiaalselt laiast ja lootusrikkast kontseptuaalsest kategooriast järjest enam paindumatuks, piiravaks ja igavaks vanade tavade järgimiseks

  3. Notch signaling activation in human embryonic stem cells is required for embryonic but not trophoblastic lineage commitment

    OpenAIRE

    Yu, Xiaobing; Zou, Jizhong; Ye, Zhaohui; Hammond, Holly; Chen, Guibin; Tokunaga, Akinori; Mali, Prashant; Li, Yue-Ming; Civin, Curt; Gaiano, Nicholas; Cheng, Linzhao

    2008-01-01

    The Notch signaling pathway plays important roles in cell fate determination during embryonic development and adult life. In this study, we focus on the role of Notch signaling in governing cell fate choices in human embryonic stem (hES) cells. Using genetic and pharmacological approaches, we achieved both blockade and conditional activation of Notch signaling in several hES cell lines. We report here that activation of Notch signaling is required for undifferentiated hES cells to form the pr...

  4. A Case Report of Eosinophilic Esophagitis Accompanying Hypereosinophilic Syndrome

    Directory of Open Access Journals (Sweden)

    Mahreema Jawairia

    2012-01-01

    Full Text Available Hypereosinophilic syndrome is a blood disorder characterized by the overproduction of eosinophils in the bone marrow with persistent peripheral eosinophilia, associated with organ damage by the release of eosinophilic mediators. Although HES can involve multiple organ systems, GI tract involvement is very rare. Few cases of HES presenting with gastritis or enteritis have been reported worldwide. To date, HES presenting with esophagus involvement has only been reported once. Here, we present a 39-year-old Hispanic female patient with history of HES presenting with complaints of dysphagia and generalized pruritus.

  5. Changes in circulating blood volume after infusion of hydroxyethyl starch 6% in critically ill patients

    DEFF Research Database (Denmark)

    Christensen, P; Andersson, J; Rasmussen, S E

    2001-01-01

    The cardiovascular response to a volume challenge with hydroxyethyl starch (HES) (200/0.5) 6% depends on the relation between the volume of HES 6% infused and the expansion of the blood volume in critically ill patients. However, only relatively limited data exist on the plasma expanding effect...... of infusion of HES 6% in critically ill patients. The purpose of the study was to evaluate the variation in the expansion of the circulating blood volume (CBV) in critically ill patients after infusion of 500 ml of colloid (HES (200/0.5) 6%) using the carbon monoxide method....

  6. Vascular disease in women: comparison of diagnoses in hospital episode statistics and general practice records in England

    Directory of Open Access Journals (Sweden)

    Wright F

    2012-10-01

    Full Text Available Abstract Background Electronic linkage to routine administrative datasets, such as the Hospital Episode Statistics (HES in England, is increasingly used in medical research. Relatively little is known about the reliability of HES diagnostic information for epidemiological studies. In the United Kingdom (UK, general practitioners hold comprehensive records for individuals relating to their primary, secondary and tertiary care. For a random sample of participants in a large UK cohort, we compared vascular disease diagnoses in HES and general practice records to assess agreement between the two sources. Methods Million Women Study participants with a HES record of hospital admission with vascular disease (ischaemic heart disease [ICD-10 codes I20-I25], cerebrovascular disease [G45, I60-I69] or venous thromboembolism [I26, I80-I82] between April 1st 1997 and March 31st 2005 were identified. In each broad diagnostic group and in women with no such HES diagnoses, a random sample of about a thousand women was selected for study. We asked each woman’s general practitioner to provide information on her history of vascular disease and this information was compared with the HES diagnosis record. Results Over 90% of study forms sent to general practitioners were returned and 88% of these contained analysable data. For the vast majority of study participants for whom information was available, diagnostic information from general practice and HES records was consistent. Overall, for 93% of women with a HES diagnosis of vascular disease, general practice records agreed with the HES diagnosis; and for 97% of women with no HES diagnosis of vascular disease, the general practitioner had no record of a diagnosis of vascular disease. For severe vascular disease, including myocardial infarction (I21-22, stroke, both overall (I60-64 and by subtype, and pulmonary embolism (I26, HES records appeared to be both reliable and complete. Conclusion Hospital admission data

  7. Afganistan näitab välismaalasele oma rahulikku nägu, kuni midagi ohtlikku ei juhtu / Aivar Aotäht

    Index Scriptorium Estoniae

    Aotäht, Aivar, 1970-

    2008-01-01

    Ajakirjaniku muljed Afganistanis ja kohtumisest Afgaani politseijuhte nõustava EL politseimissiooni poliitnõuniku Andres Kolgi, Afganistani politsei arendamise nõustaja Jaan Helmdorfi ja ISAF-i kontaktohvitseri Ülar Lõhmusega. Vt. samas: Pilguheit pealinnale teise nurga alt. Artiklis tutvustab Eesti kaitseministeeriumi nõunik Gerri Lesk, kelle ülesanne on nõustada Afganistani kaitseministeeriumi, ajakirjanikele NATO sõjaväebaasi ja Afganistani pealinna Kabuli

  8. Äppideteemaline vestlusring: lapsed ei lähe enam iga hullusega kaasa / Kristjan Port, Kristi Mäe, Kristi Rahn, Jakob Rosin ; intervjueerinud Madis Aesma

    Index Scriptorium Estoniae

    2016-01-01

    Käib konkurss "Eesti parim mobiilirakendus 2016". Vestlusringis äppidest rääkimas inimesed, kelle jaoks rakendused mängivad igapäevaelus erinevaid rolle - spordibioloog, SA Eesti Antidoping nõukogu liige Kristjan Port, politsei- ja piirivalveameti juhtivkriminaalametnik Kristi Mäe, Gustav Adolfi gümnaasiumi klassiõpetaja Kristi Rahn ja Eesti pimedate liidu juhatuse liige, vaegnägija Jakob Rosin

  9. Aitab hädaldamisest / Robert Antropov

    Index Scriptorium Estoniae

    Antropov, Robert, 1965-

    2006-01-01

    Endine politseijuht on seisukohal, et politseinike ja päästjate nappuses ei saa süüdistada ainult väikseid palku, küsimus on valikutes: kuidas ja millesse ressursse paigutada. Kalle Laanet ja Raivo Aeg vastavad küsimusele, mis on nende haldusalas konkreetselt kavas, et politsei saaks täita oma tegevusprioriteeti 100% ja tagada sisejulgeoleku. Vt. ka Robert Antropovi kommentaari 7. aug. Eesti Päevaleht lk. 2

  10. Kaitseliidu kasutamine mässutõrjeoperatsioonidel / Riho Ühtegi

    Index Scriptorium Estoniae

    Ühtegi, Riho, 1964-

    2007-01-01

    Ülevaade mässutõrjeoperatsioonidest nii ülemaailmses kui Eesti kontekstis, nende eripärast ning kehtestatud nõuetest, Kaitseliidu rakendamise võimalustest sellistes operatsioonides. Lisa: Mässutõrjeoperatsioonid (coin-operatsioonid, counter-insurgency operations); Mässutõrjeoperatsioonide nõrkused; Iraagi armee ja politsei mässutõrjeoperatsioonide taktika; Mässuliste neid toetavast keskkonnast isoleerimise kolm põhiprintsiipi; Mässutõrje paradoksid

  11. Hydroxyethyl starch 130/0.42 versus Ringer's acetate in severe sepsis

    DEFF Research Database (Denmark)

    Perner, Anders; Haase, Nicolai; Guttormsen, Anne B

    2012-01-01

    Hydroxyethyl starch (HES) [corrected] is widely used for fluid resuscitation in intensive care units (ICUs), but its safety and efficacy have not been established in patients with severe sepsis.......Hydroxyethyl starch (HES) [corrected] is widely used for fluid resuscitation in intensive care units (ICUs), but its safety and efficacy have not been established in patients with severe sepsis....

  12. Hydroxyethyl Starch Reduces Coagulation Competence and Increases Blood Loss During Major Surgery

    DEFF Research Database (Denmark)

    Rasmussen, Kirsten C; Johansson, Pär I; Højskov, Michael

    2014-01-01

    OBJECTIVE: This study evaluated whether administration of hydroxyethyl starch (HES) 130/0.4 affects coagulation competence and influences the perioperative blood loss. BACKGROUND: Artificial colloids substitute blood volume during surgery; with the administration of HES 130/0.4 (Voluven, Fresenius...

  13. Assessment of the Potential of CDK2 Inhibitor NU6140 to Influence the Expression of Pluripotency Markers NANOG, OCT4, and SOX2 in 2102Ep and H9 Cells

    Directory of Open Access Journals (Sweden)

    Ade Kallas

    2014-01-01

    Full Text Available As cyclin-dependent kinases (CDKs regulate cell cycle progression and RNA transcription, CDKs are attractive targets for creating cancer cell treatments. In this study we investigated the effects of the small molecular agent NU6140 (inhibits CDK2 and cyclin A interaction on human embryonic stem (hES cells and embryonal carcinoma-derived (hEC cells via the expression of transcription factors responsible for pluripotency. A multiparameter flow cytometric method was used to follow changes in the expression of NANOG, OCT4, and SOX2 together in single cells. Both hES and hEC cells responded to NU6140 treatment by induced apoptosis and a decreased expression of NANOG, OCT4, and SOX2 in surviving cells. A higher sensitivity to NU6140 application in hES than hEC cells was detected. NU6140 treatment arrested hES and hEC cells in the G2 phase and inhibited entry into the M phase as evidenced by no significant increase in histone 3 phosphorylation. When embryoid bodies (EBs formed from NU6104 treated hES cells were compared to EBs from untreated hES cells differences in ectodermal, endodermal, and mesodermal lineages were found. The results of this study highlight the importance of CDK2 activity in maintaining pluripotency of hES and hEC cells and in differentiation of hES cells.

  14. Moving Mexico’s Higher Education System Forward -- Potential and Challenges

    Science.gov (United States)

    2011-10-28

    Mexican HES. The Mexican populace generally possesses a deeply embedded cultural aversion to self-assessment, especially when it comes to education...throughout the 31 states that make up the country.5 The Mexican public HES is nominally administered from Mexico City by the Secretaria de Educacion

  15. NCBI nr-aa BLAST: CBRC-ETEL-01-0698 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-0698 ref|YP_441274.1| HesA/MoeB/ThiF family protein [Burkholderia thailand...ensis E264] gb|ABC38646.1| HesA/MoeB/ThiF family protein [Burkholderia thailandensis E264] YP_441274.1 0.73 41% ...

  16. Acute kidney injury with hydroxyethyl starch 130/0.42 in severe sepsis

    DEFF Research Database (Denmark)

    Müller, Rasmus Gamborg; Haase, Nicolai; Lange, T

    2015-01-01

    BACKGROUND: We aimed to detail the effects of hydroxyethyl starch (HES) vs. Ringer's on kidney function including the interaction with mortality in post-hoc analyses as resuscitation with HES 130/0.42 increased mortality in the Scandinavian Starch for Severe Sepsis/Septic Shock (6S) trial. METHODS...

  17. Löffler's endomyocarditis in the idiopathic hypereosinophilic syndrome

    NARCIS (Netherlands)

    Corssmit, E. P.; Trip, M. D.; Durrer, J. D.

    1999-01-01

    The idiopathic hypereosinophilic syndrome (HES) is a leukoproliferative disorder characterized by sustained eosinophilia (> 1.5 x 10(9)/l) and (multi-)organ dysfunction caused by infiltration of eosinophils. Especially the heart is frequently affected. In this report, we describe 2 patients with HES

  18. Radioprotective effects of hesperidin on oxidative damages and histopathological changes induced by X-irradiation in rats heart tissue

    Directory of Open Access Journals (Sweden)

    Abolhasan Rezaeyan

    2016-01-01

    Full Text Available This study was carried out to evaluate radioprotective effects of hesperidin (HES administration before the irradiation on the cardiac oxidative stress and histopathological changes in an experimental rat model. The cardiovascular complications of radiation exposure cause morbidity and mortality in patients who received radiotherapy. HES, an antioxidant flavonoid found in citrus fruits, suggests the protection against the tissue damage. Fifty-eight rats were divided into four groups: Group 1 received phosphate buffered saline (PBS and sham radiation; Group 2, HES and sham radiation; Group 3, PBS and radiation; and Group 4, HES and radiation. The rats were exposed to single dose of 18 Gy of 6 MV X-ray. One hundred milligrams per kilogram doses of HES was administered for 7 days before irradiation. The estimation of superoxide dismutase (SOD, malondialdehyde (MDA, and histopathological analyses was performed at 24 h and 8 weeks after radiation exposure. The irradiation of chest area resulted in an elevated MDA level and decreased SOD activity. Moreover, long-term pathological lesions of radiation were inflammation, fibrosis, the increased number of mast cells and macrophages, and development of plaque, vascular leakage, myocardial degeneration, and myocyte necrosis. Although the administration of HES decreases inflammation, fibrosis, mast cell and macrophage numbers, and myocyte necrosis, it did not result in reduced thrombus, myocardium degeneration, and vascular leakage. In conclusion, these results suggest that HES can perform a radioprotection action. The protective effect of HES may be attributable to its immunomodulatory effects and free radical-scavenging properties.

  19. Evaluation of the photovoltaic power plant of the GridLab

    OpenAIRE

    Wyer, Kevin; Genoud, Dominique

    2016-01-01

    In der vorliegenden Bachelorarbeit wird eine Software beziehungsweise ein Überwa- chungssystem für die Fachhochschule HES-SO in Sion implementiert. Mithilfe dieses Über-wachungssystems kann die produzierte Energie der Photovoltaikanlage am Gebäude der HES-SO Sion grafisch angezeigt werden.

  20. Identifying developmental toxicity pathways for a subset of ToxCast chemicals using human embryonic stem cells and metabolomics

    Science.gov (United States)

    Metabolomics analysis was performed on the supernatant of human embryonic stem (hES) cell cultures exposed to a blinded subset of 11 chemicals selected from the chemical library of EPA's ToxCast™ chemical screening and prioritization research project. Metabolites from hES cultur...

  1. Chemical Reactivity Test (CRT)

    Energy Technology Data Exchange (ETDEWEB)

    Zaka, F. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-12-13

    The Chemical Reactivity Test (CRT) is used to determine the thermal stability of High Explosives (HEs) and chemical compatibility between (HEs) and alien materials. The CRT is one of the small-scale safety tests performed on HE at the High Explosives Applications Facility (HEAF).

  2. Physically crosslinked poly(vinyl alcohol-hydroxyethyl starch blend hydrogel membranes: Synthesis and characterization for biomedical applications

    Directory of Open Access Journals (Sweden)

    El-Refaie Kenawy

    2014-07-01

    Full Text Available Poly(vinyl alcohol, PVA is a polymer of great importance because of its many appealing characteristics specifically for various pharmaceutical and biomedical applications. Physically crosslinked hydrogel membranes composed of different amounts of hydroxyethyl starch (HES in (PVA and ampicillin were prepared by applying freeze–thawing method. This freezing–thawing cycle was repeated for three consecutive cycles. Physicochemical properties of PVA–HES membrane gel such as gel fraction, swelling, morphology, elongation, tensile strength, and protein adsorption were investigated. Introducing HES into freeze–thawed PVA structure affected crystal size distribution of PVA; and hence physicochemical properties and morphological structure have been affected. Increased HES concentration decreased the gel fraction %, maximum strength and break elongation. Indeed it resulted into a significant incrementing of the swelling ability, amount of protein adsorption, broader pore size, and pore distribution of membrane morphological structure. Furthermore, an increase in HES concentration resulted in better and still lower thermal stability compared to virgin PVA and freeze–thawed PVA. The maximum weight loss of PVA–HES hydrogel membranes ranged between 18% and 60% according to HES content, after two days of degradation in phosphate buffer saline (PBS, which indicates they are biodegradable. Thus, PVA–HES hydrogel membranes containing ampicillin could be a novel approach for biomedical application e.g. wound dressing purposes.

  3. Kas keegi vajab neid raamatuid? / Jaanika Palm

    Index Scriptorium Estoniae

    Palm, Jaanika, 1973-

    2001-01-01

    Rets. rmt. : Müürsepp, Mare. Kolm põrsakest. Punamütsike : muinasjutu-kirjavihik 5-aastastele lastele. 1 vihik / jutustanud, luuletanud ja ülesanded välja mõelnud Mare Müürsepp. Tallinn : Ilo, 2000. Sari "Muinasjutt õpetab" : Hans ja Grete / [idee: Nicole Lenaerts-Viroux. Tallinn] : Egmont Estonia, 2001; Hunt ja seitse kitsetalle / [idee: Nicole Lenaerts-Viroux. Tallinn] : Egmont Estonia, 2000; Lumivalgeke ja seitse pöialpoissi / [idee: Nicole Lenaerts-Viroux. Tallinn] : Egmont Estonia, 2001; Pöial-Liisi / [idee: Nicole Lenaerts-Viroux. Tallinn] : Egmont Estonia, 2000

  4. Initial administration of hydroxyethyl starch vs lactated Ringer after liver trauma in the pig

    DEFF Research Database (Denmark)

    Zaar, M.; Lauritzen, B.; Secher, Niels H.

    2009-01-01

    simulated an acute pre-hospital event: after a standard first-respond delay (7 min), volume administration was provided in three phases to simulate increasing intravascular access. In the first two phases, the fluid was administered either by HES or by RL and, during the last phase, all animals received HES......BACKGROUND: This study tested the circulatory effectiveness of post-trauma administration of a large intravascular volume expander, hydroxyethyl starch 130/0.4 (HES), vs standard lactated Ringer's solution (RL). METHODS: Liver injury was inflicted in 14 pigs [31 (4) kg; mean (sd)] and treatment......)% for HES and 76 (21)% for RL (Padministration of HES provoked uncontrolled bleeding, whereas the administration of RL...

  5. Effect of tetrastarch (hydroxyethyl starch 130/0.4) on plasma creatinine concentration in cats: a retrospective analysis (2010-2015).

    Science.gov (United States)

    Yozova, Ivayla D; Howard, Judith; Adamik, Katja N

    2017-10-01

    Objectives The objective was to determine survival and changes in creatinine concentrations after administration of 6% tetrastarch (hydroxyethyl starch [HES] 130/0.4) vs crystalloids in critically ill cats. Methods The medical records were reviewed for cats admitted to the intensive care unit with at least two plasma creatinine measurements and initial concentrations not exceeding the upper reference interval. Cats were excluded if they had received HES prior to admission or if they had received fluid therapy for creatinine concentrations were evaluated as the percentage change from initial values to the maximum subsequent measurements. Cats receiving only crystalloids were assigned to the crystalloid group; cats receiving only HES or HES and crystalloids were assigned to the HES group. Results Ninety-three cats were included in the study (62 in the crystalloid group, 31 in the HES group). The total median cumulative HES dose was 94 ml/kg (range 26-422 ml/kg) and 24 ml/kg/day (range 16-42 ml/kg/day). No difference was detected between the groups for age, sex, body weight or mortality. The HES group had a significantly longer length of hospitalisation ( P = 0.012), lower albumin concentrations ( P creatinine concentrations between the groups. Moreover, there was no significant difference in maximum change in creatinine concentrations in the subgroups of cats with systemic inflammatory response syndrome or sepsis. Conclusions and relevance In this population of cats, the administration of HES did not result in a significantly greater increase in creatinine from values measured on admission or higher mortality compared with administration of crystalloids. Further prospective studies are needed to assess both safety and efficacy of HES in cats before recommendations can be made.

  6. Human fetal liver stromal cells that overexpress bFGF support growth and maintenance of human embryonic stem cells.

    Directory of Open Access Journals (Sweden)

    Jiafei Xi

    Full Text Available In guiding hES cell technology toward the clinic, one key issue to be addressed is to culture and maintain hES cells much more safely and economically in large scale. In order to avoid using mouse embryonic fibroblasts (MEFs we isolated human fetal liver stromal cells (hFLSCs from 14 weeks human fetal liver as new human feeder cells. hFLSCs feeders could maintain hES cells for 15 passages (about 100 days. Basic fibroblast growth factor (bFGF is known to play an important role in promoting self-renewal of human embryonic stem (hES cells. So, we established transgenic hFLSCs that stably express bFGF by lentiviral vectors. These transgenic human feeder cells--bFGF-hFLSCs maintained the properties of H9 hES cells without supplementing with any exogenous growth factors. H9 hES cells culturing under these conditions maintained all hES cell features after prolonged culture, including the developmental potential to differentiate into representative tissues of all three embryonic germ layers, unlimited and undifferentiated proliferative ability, and maintenance of normal karyotype. Our results demonstrated that bFGF-hFLSCs feeder cells were central to establishing the signaling network among bFGF, insulin-like growth factor 2 (IGF-2, and transforming growth factor β (TGF-β, thereby providing the framework in which hES cells were instructed to self-renew or to differentiate. We also found that the conditioned medium of bFGF-hFLSCs could maintain the H9 hES cells under feeder-free conditions without supplementing with bFGF. Taken together, bFGF-hFLSCs had great potential as feeders for maintaining pluripotent hES cell lines more safely and economically.

  7. Enhanced transfection efficiency of human embryonic stem cells by the incorporation of DNA liposomes in extracellular matrix.

    Science.gov (United States)

    Villa-Diaz, Luis G; Garcia-Perez, Jose L; Krebsbach, Paul H

    2010-12-01

    Because human embryonic stem (hES) cells can differentiate into virtually any cell type in the human body, these cells hold promise for regenerative medicine. The genetic manipulation of hES cells will enhance our understanding of genes involved in early development and will accelerate their potential use and application for regenerative medicine. The objective of this study was to increase the transfection efficiency of plasmid DNA into hES cells by modifying a standard reverse transfection (RT) protocol of lipofection. We hypothesized that immobilization of plasmid DNA in extracellular matrix would be a more efficient method for plasmid transfer due to the affinity of hES cells for substrates such as Matrigel and to the prolonged exposure of cells to plasmid DNA. Our results demonstrate that this modification doubled the transfection efficiency of hES cells and the generation of clonal cell lines containing a piece of foreign DNA stably inserted in their genomes compared to results obtained with standard forward transfection. In addition, treatment with dimethyl sulfoxide further increased the transfection efficiency of hES cells. In conclusion, modifications to the RT protocol of lipofection result in a significant and robust increase in the transfection efficiency of hES cells.

  8. Persistent expression of activated notch in the developing hypothalamus affects survival of pituitary progenitors and alters pituitary structure.

    Science.gov (United States)

    Aujla, Paven K; Bogdanovic, Vedran; Naratadam, George T; Raetzman, Lori T

    2015-08-01

    As the pituitary gland develops, signals from the hypothalamus are necessary for pituitary induction and expansion. Little is known about the control of cues that regulate early signaling between the two structures. Ligands and receptors of the Notch signaling pathway are found in both the hypothalamus and Rathke's pouch. The downstream Notch effector gene Hes1 is required for proper pituitary formation; however, these effects could be due to the action of Hes1 in the hypothalamus, Rathke's pouch, or both. To determine the contribution of hypothalamic Notch signaling to pituitary organogenesis, we used mice with loss and gain of Notch function within the developing hypothalamus. We demonstrate that loss of Notch signaling by conditional deletion of Rbpj in the hypothalamus does not affect expression of Hes1 within the posterior hypothalamus or expression of Hes5. In contrast, expression of activated Notch within the hypothalamus results in ectopic Hes5 expression and increased Hes1 expression, which is sufficient to disrupt pituitary development and postnatal expansion. Taken together, our results indicate that Rbpj-dependent Notch signaling within the developing hypothalamus is not necessary for pituitary development, but persistent Notch signaling and ectopic Hes5 expression in hypothalamic progenitors affects pituitary induction and expansion. © 2015 Wiley Periodicals, Inc.

  9. Roles of bHLH genes in neural stem cell differentiation

    International Nuclear Information System (INIS)

    Kageyama, Ryoichiro; Ohtsuka, Toshiyuki; Hatakeyama, Jun; Ohsawa, Ryosuke

    2005-01-01

    Neural stem cells change their characteristics over time during development: they initially proliferate only and then give rise to neurons first and glial cells later. In the absence of the repressor-type basic helix-loop-helix (bHLH) genes Hes1, Hes3 and Hes5, neural stem cells do not proliferate sufficiently but prematurely differentiate into neurons and become depleted without making the later born cell types such as astrocytes and ependymal cells. Thus, Hes genes are essential for maintenance of neural stem cells to make cells not only in correct numbers but also in full diversity. Hes genes antagonize the activator-type bHLH genes, which include Mash1, Math and Neurogenin. The activator-type bHLH genes promote the neuronal fate determination and induce expression of Notch ligands such as Delta. These ligands activate Notch signaling and upregulate Hes1 and Hes5 expression in neighboring cells, thereby maintaining these cells undifferentiated. Thus, the activator-type and repressor-type bHLH genes regulate each other, allowing only subsets of cells to undergo differentiation while keeping others to stay neural stem cells. This regulation is essential for generation of complex brain structures of appropriate size, shape and cell arrangement

  10. Omega-3 Polyunsaturated Fatty Acids Enhance Neuronal Differentiation in Cultured Rat Neural Stem Cells

    Directory of Open Access Journals (Sweden)

    Masanori Katakura

    2013-01-01

    Full Text Available Polyunsaturated fatty acids (PUFAs can induce neurogenesis and recovery from brain diseases. However, the exact mechanisms of the beneficial effects of PUFAs have not been conclusively described. We recently reported that docosahexaenoic acid (DHA induced neuronal differentiation by decreasing Hes1 expression and increasing p27kip1 expression, which causes cell cycle arrest in neural stem cells (NSCs. In the present study, we examined the effect of eicosapentaenoic acid (EPA and arachidonic acid (AA on differentiation, expression of basic helix-loop-helix transcription factors (Hes1, Hes6, and NeuroD, and the cell cycle of cultured NSCs. EPA also increased mRNA levels of Hes1, an inhibitor of neuronal differentiation, Hes6, an inhibitor of Hes1, NeuroD, and Map2 mRNA and Tuj-1-positive cells (a neuronal marker, indicating that EPA induced neuronal differentiation. EPA increased the mRNA levels of p21cip1 and p27kip1, a cyclin-dependent kinase inhibitor, which indicated that EPA induced cell cycle arrest. Treatment with AA decreased Hes1 mRNA but did not affect NeuroD and Map2 mRNA levels. Furthermore, AA did not affect the number of Tuj-1-positive cells or cell cycle progression. These results indicated that EPA could be involved in neuronal differentiation by mechanisms alternative to those of DHA, whereas AA did not affect neuronal differentiation in NSCs.

  11. Hemodynamic effects of 6% hydroxyethyl starch infusion in sevoflurane-anesthetized thoroughbred horses.

    Science.gov (United States)

    Ohta, Minoru; Kurimoto, Shinjiro; Tokushige, Hirotaka; Kuroda, Taisuke; Ishikawa, Yuhiro

    2013-07-31

    To determine hemodynamic effects of hydroxyethyl starch (HES) infusion during anesthesia in horses, incremental doses of 6% HES were administered to 6 healthy Thoroughbred horses. Anesthesia was induced with xylazine, guaifenesin and thiopental and maintained with sevoflurane at 2.8% of end-tidal concentration in all horses. The horses were positioned in right lateral recumbency and administered 3 intravenous dose of 6% HES (5 ml/kg) over 15 min with 15-min intervals in addition to constant infusion of lactated Ringer's solution at 10 ml/kg/hr. Hemodynamic parameters were measured before and every 15 min until 90 min after the administration of 6% HES. There was no significant change in heart rate and arterial blood pressures throughout the experiment. The HES administration produced significant increases in mean right atrial pressure, stroke volume, cardiac output (CO) and decrease in systemic vascular resistance (SVR) in a dose-dependent manner. There was no significant change in electrolytes (Na(+), K(+), Cl(-)) throughout the experiment, however, packed cell volume, hemoglobin concentration, and total protein and albumin concentrations decreased in a dose-dependent manner following the HES administration. In conclusion, the HES administration provides a dose-dependent increase in CO, but has no impact upon arterial blood pressures due to a simultaneous decrease in SVR.

  12. Less impairment of hemostasis and reduced blood loss in pigs after resuscitation from hemorrhagic shock using the small-volume concept with hypertonic saline/hydroxyethyl starch as compared to administration of 4% gelatin or 6% hydroxyethyl starch solution.

    Science.gov (United States)

    Haas, Thorsten; Fries, Dietmar; Holz, Carmen; Innerhofer, Petra; Streif, Werner; Klingler, Anton; Hanke, Alexander; Velik-Salchner, Corinna

    2008-04-01

    Small-volume resuscitation using hypertonic saline/hydroxyethyl starch 200/0.62 (HS-HES) has been shown to be an effective alternative to the administration of crystalloids or colloids in trauma patients. All i.v. fluids cause dose-related dilutional coagulopathy and show intrinsic effects on the hemostatic system, but only few data refer to functional consequences after small-volume resuscitation. Using thrombelastometry (ROTEM), we studied 30 pigs (weighing 35-45 kg) after withdrawal of 60% of blood volume [1484 mL (1369-1624 mL)] and receiving 4 mL/kg HS-HES for compensation of blood loss or 4% gelatin or 6% HES 130/0.4 in a 1:1 ratio to lost blood volume. To compare the ROTEM variables (coagulation time, clot formation time, alpha angle, clot firmness, and fibrinogen polymerization) with bleeding tendency, a hepatic incision was made and blood loss was measured. Median (25th, 75th percentile) fibrinogen polymerization was significantly higher after HS-HES infusion [11 mm (10, 11), P = 0.0034] when compared with administration of 4% gelatin [4.5 mm (3.0, 5.8)] or HES 130/0.4 [3.5 mm (2.3, 4.0)]. Median blood loss after liver incision was 725 mL (900, 375) after HS-HES, 1625 mL (1275, 1950) after 4% gelatin, and 1600 mL (1500, 1800) after 6% HES 130/0.4 (P = 0.004). Hemodynamic stabilization was traceable in all groups but showed differences regarding filling pressures. Resuscitation from hemorrhagic shock with HS-HES 200/0.62 results in less impairment of clot formation when compared with compensation of blood loss by administering 6% HES 130/0.4 or 4% gelatin.

  13. Cryopreservation of dermal fibroblasts and keratinocytes in hydroxyethyl starch-based cryoprotectants.

    Science.gov (United States)

    Naaldijk, Yahaira; Johnson, Adiv A; Friedrich-Stöckigt, Annett; Stolzing, Alexandra

    2016-12-01

    Preservation of human skin fibroblasts and keratinocytes is essential for the creation of skin tissue banks. For successful cryopreservation of cells, selection of an appropriate cryoprotectant agent (CPA) is imperative. The aim of this study was to identify CPAs that minimize toxic effects and allow for the preservation of human fibroblasts and keratinocytes in suspension and in monolayers. We cryopreserved human fibroblasts and keratinocytes with different CPAs and compared them to fresh, unfrozen cells. Cells were frozen in the presence and absence of hydroxyethyl starch (HES) or dimethyl sulfoxide (DMSO), the latter of which is a commonly used CPA known to exert toxic effects on cells. Cell numbers were counted immediately post-thaw as well as three days after thawing. Cellular structures were analyzed and counted by labeling nuclei, mitochondria, and actin filaments. We found that successful cryopreservation of suspended or adherent keratinocytes can be accomplished with a 10% HES or a 5% HES, 5% DMSO solution. Cell viability of fibroblasts cryopreserved in suspension was maintained with 10% HES or 5% HES, 5% DMSO solutions. Adherent, cryopreserved fibroblasts were successfully maintained with a 5% HES, 5% DMSO solution. We conclude that skin tissue cells can be effectively cryopreserved by substituting all or a portion of DMSO with HES. Given that DMSO is the most commonly used CPA and is believed to be more toxic than HES, these findings are of clinical significance for tissue-based replacement therapies. Therapies that require the use of keratinocyte and fibroblast cells, such as those aimed at treating skin wounds or skin burns, may be optimized by substituting a portion or all of DMSO with HES during cryopreservation protocols.

  14. Comparing the effect of hydroxyethyl starch 130/0.4 with balanced crystalloid solution on mortality and kidney failure in patients with severe sepsis (6S - Scandinavian Starch for Severe Sepsis/Septic Shock trial): Study protocol, design and rationale for a double-blinded, randomised clinical trial

    DEFF Research Database (Denmark)

    Perner, Anders; Haase, Nicolai; Wetterslev, Jørn

    2011-01-01

    ABSTRACT: BACKGROUND: By tradition colloid solutions have been used to obtain fast circulatory stabilisation in shock, but high molecular weight hydroxyethyl starch (HES) may cause acute kidney failure in patients with severe sepsis. Now lower molecular weight HES 130/0.4 is the preferred colloid...... of HES 130/0.4 in patients with severe sepsis. The effects on mortality, dialysis-dependency, time on ventilator, bleeding and markers of resuscitation, metabolism, kidney failure, and coagulation will be assessed. TRIAL REGISTRATION: ClinicalTrials.gov: NCT00962156....

  15. Colloids in Cardiac Surgery-Friend or Foe?

    DEFF Research Database (Denmark)

    Ryhammer, Pia Katarina; Tang, Mariann; Hoffmann-Petersen, Joachim

    2017-01-01

    score matching. SETTING: Cohort study from 3 university hospitals using a common registry. PARTICIPANTS: The study comprised 17,742 patients who were referred for cardiac surgery from 2007 to 2014. INTERVENTIONS: Patients were divided in groups according to perioperative fluid replacement with either...... crystalloids or colloids. The colloid group was further divided into HES or human albumin (HA). Analyses were based on the following 3 subsections: HES versus crystalloids, HA versus crystalloids, and HES versus HA, with use of propensity score matching or direct matching of cases. Primary outcome parameters...

  16. Modelling of a Hybrid Energy System for Autonomous Application

    Directory of Open Access Journals (Sweden)

    Yang He

    2013-10-01

    Full Text Available A hybrid energy system (HES is a trending power supply solution for autonomous devices. With the help of an accurate system model, the HES development will be efficient and oriented. In spite of various precise unit models, a HES system is hardly developed. This paper proposes a system modelling approach, which applies the power flux conservation as the governing equation and adapts and modifies unit models of solar cells, piezoelectric generators, a Li-ion battery and a super-capacitor. A generalized power harvest, storage and management strategy is also suggested to adapt to various application scenarios.

  17. Hydroxyethyl starch 130/0.42/6:1 for perioperative plasma volume replacement in 1130 children: results of an European prospective multicenter observational postauthorization safety study (PASS)

    NARCIS (Netherlands)

    Sümpelmann, Robert; Kretz, Franz-Josef; Luntzer, Robert; de Leeuw, Thomas G.; Mixa, Vladimir; Gäbler, Ralf; Eich, Christoph; Hollmann, Markus W.; Osthaus, Wilhelm A.

    2012-01-01

    Introduction: Third-generation hydroxyethyl starch (HES) is now approved also for the use in children, but safety studies including large numbers of pediatric patients are still missing. Therefore, we performed an European multicentric prospective observational postauthorization safety study (PASS)

  18. Guidelines for human embryonic stem cell research

    National Research Council Canada - National Science Library

    Committee on Guidelines for Human Embryonic Stem Cell Research; National Research Council; Board on Health Sciences Policy; Institute of Medicine; Division on Earth and Life Studies; National Research Council

    2005-01-01

    .... Given limited federal involvement, privately funded hES cell research has thus far been carried out under a patchwork of existing regulations, many of which were not designed with this research specifically in mind...

  19. Notch signaling inhibitor DAPT provides protection against acute craniocerebral injury.

    Directory of Open Access Journals (Sweden)

    Hong-Mei Zhang

    Full Text Available Notch signaling pathway is involved in many physiological and pathological processes. The γ-secretase inhibitor DAPT inhibits Notch signaling pathway and promotes nerve regeneration after cerebral ischemia. However, neuroprotective effects of DAPT against acute craniocerebral injury remain unclear. In this study, we established rat model of acute craniocerebral injury, and found that with the increase of damage grade, the expression of Notch and downstream protein Hes1 and Hes5 expression gradually increased. After the administration of DAPT, the expression of Notch, Hes1 and Hes5 was inhibited, apoptosis and oxidative stress decreased, neurological function and cognitive function improved. These results suggest that Notch signaling can be used as an indicator to assess the severity of post-traumatic brain injury. Notch inhibitor DAPT can reduce oxidative stress and apoptosis after acute craniocerebral injury, and is a potential drug for the treatment of acute craniocerebral injury.

  20. Avariide kiuste Monte Carlosse / Aare Arula

    Index Scriptorium Estoniae

    Arula, Aare

    2007-01-01

    Vt. ka Tehnika dlja Vsehh nr. 3, lk. 26-27. 26. jaanuaril 1937 Tallinnast Monte Carlo tähesõidule startinud Karl Siitanit ja tema meeskonda ootasid ees seiklused, mis oleksid neile peaaegu elu maksnud

  1. Manipulation of the extracellular microenvironment by micro- and nanotechnology approaches to improve the generation of pancreatic endocrine cells from human embryonic stem cells

    DEFF Research Database (Denmark)

    Rasmussen, Camilla Holzmann; Rønn Petersen, Dorthe

    ) alteration of substrate topography and elasticity (paper 2) and 3) initial seeding and cell distribution (paper 3). With the first strategy an array screen was performed to systematically identify ECM protein coatings, which induced or inhibited the differentiation of hES cells towards DE. Almost 500...... different ECM protein combinations were screened and several candidates were found. The majority of these candidates could be validated in microtitre well plates and further studies demonstrated that certain ECM proteins regulate the differentiation of hES cells towards DE. Netrin 1, collagen 1 and collagen...... characters. The study consisted of an investigation of the differentiation of hES cells towards DE and PE on nanopillars. The nanopillars with soft character were not favoured by hES cells with regards to attachment and growth. However, DE cells appeared earlier during the DE differentiation on the soft...

  2. Guidelines for human embryonic stem cell research

    National Research Council Canada - National Science Library

    Committee on Guidelines for Human Embryonic Stem Cell Research, National Research Council

    2005-01-01

    Since 1998, the volume of research being conducted using human embryonic stem (hES) cells has expanded primarily using private funds because of restrictions on the use of federal funds for such research...

  3. EMMPRIN is secreted by human uterine epithelial cells in microvesicles and stimulates metalloproteinase production by human uterine fibroblast cells.

    Science.gov (United States)

    Braundmeier, A G; Dayger, C A; Mehrotra, P; Belton, R J; Nowak, R A

    2012-12-01

    Endometrial remodeling is a physiological process involved in the gynecological disease, endometriosis. Tissue remodeling is directed by uterine fibroblast production of matrix metalloproteinases (MMPs). Several MMPs are regulated directly by the protein extracellular matrix metalloproteinase inducer (EMMPRIN) and also by proinflammatory cytokines such as interleukin (IL)1-α/β. We hypothesized that human uterine epithelial cells (HESs) secrete intact EMMPRIN to stimulate MMPs. Microvesicles from HES cell-conditioned medium (CM) expressed intact EMMPRIN protein. Treatment of HES cells with estradiol or phorbyl 12-myristate-13-acetate increased the release of EMMPRIN-containing microvesicles. The HES CM stimulated MMP-1, -2, and -3 messenger RNA levels in human uterine fibroblasts (HUFs) and EMMPRIN immunodepletion from HES-cell concentrated CM reduced MMP stimulation (P EMMPRIN, in response to ovarian hormones, proinflammatory cytokines as well as activation of protein kinase C.

  4. Hybrid Energy System Modeling in Modelica

    Energy Technology Data Exchange (ETDEWEB)

    William R. Binder; Christiaan J. J. Paredis; Humberto E. Garcia

    2014-03-01

    In this paper, a Hybrid Energy System (HES) configuration is modeled in Modelica. Hybrid Energy Systems (HES) have as their defining characteristic the use of one or more energy inputs, combined with the potential for multiple energy outputs. Compared to traditional energy systems, HES provide additional operational flexibility so that high variability in both energy production and consumption levels can be absorbed more effectively. This is particularly important when including renewable energy sources, whose output levels are inherently variable, determined by nature. The specific HES configuration modeled in this paper include two energy inputs: a nuclear plant, and a series of wind turbines. In addition, the system produces two energy outputs: electricity and synthetic fuel. The models are verified through simulations of the individual components, and the system as a whole. The simulations are performed for a range of component sizes, operating conditions, and control schemes.

  5. Hooaeg, mis kadus pildilt / Krista Kaldvee

    Index Scriptorium Estoniae

    Kaldvee, Krista

    2008-01-01

    Ugala teatri hooajast 2007/2008. Peatutakse etendustel "Nora/Maja", "Runar ja Kyllikki", "Põhjas", "Kolm meest paadis", "Kunst", "Ühes Eesti teises linnas", "Kolm õde", "Linnapea" ja nende kajastamisel eesti meedias

  6. Perioperative volume replacement in children undergoing cardiac surgery: albumin versus hydroxyethyl starch 130/0.4

    NARCIS (Netherlands)

    Hanart, Christophe; Khalife, Maher; de Villé, Andrée; Otte, Florence; de Hert, Stefan; van der Linden, Philippe

    2009-01-01

    OBJECTIVE: To compare 4% albumin with 6% hydroxyethyl starch (HES) 130/0.4 in terms of perioperative blood loss and intraoperative fluid requirements in children undergoing cardiac surgery. DESIGN: Prospective randomized study. SETTING: Single University Hospital. PATIENTS: Pediatric patients

  7. Sein on ees / Andres Maimik

    Index Scriptorium Estoniae

    Maimik, Andres, 1970-

    2009-01-01

    Laurent Cantet ja Françoise Begaudeau filmist "Seinte vahel", mis on pooldokumentaalne lugu probleemsest klassikollektiivist Prantsusmaa ühes riigikoolis. Filmi aluseks on kooliõpetaja Françoise Begaudeau romaan

  8. "Klass" prantsuse moodi / Jaan Ruus

    Index Scriptorium Estoniae

    Ruus, Jaan, 1938-2017

    2009-01-01

    Laurent Cantet ja Françoise Begaudeau filmist "Seinte vahel", mis on pooldokumentaalne lugu probleemsest klassikollektiivist Prantsusmaa ühes riigikoolis. Filmi aluseks on kooliõpetaja Françoise Begaudeau romaan

  9. Ere täht Britta / Britta Vahur ; interv. Jüri Muttika

    Index Scriptorium Estoniae

    Vahur, Britta, 1984-

    2006-01-01

    Rubriigis "elu ühes päevas" TV 3 uues telesarjas "Helena" peaosalist kehastav Britta Vahur endast. Lisaks sarja stsenaristi Marko Lillemägi kirjutatud "Seriaalikangelanna Helena Haas (23)", mis kirjeldab seriaali Helena päeva

  10. Simpsonid kohtuvad Potteriga / Märt Hiietamm

    Index Scriptorium Estoniae

    Hiietamm, Märt

    2003-01-01

    Matt Groeningi animaseriaali ühes osas kohtuvad Simpsonid Inglismaal J. K. Rowlingiga. Ka teistest seriaalitegijate plaanidest ja tagasivaade algusaastale. Lisaks sõnum : "Simpsonitest" saab USA pikim naljasari

  11. ETVsse jõuab "Kontori" geeniuse uus sari "Statistid" / Triin Thalheim

    Index Scriptorium Estoniae

    Thalheim, Triin, 1982-

    2006-01-01

    Briti koomik Ricky Gervais on koostöös "Kontori" kaasautori Stephen Merchantiga tegemas sarja originaalpealkirjaga "Extras". Samas ka koomiku osalemisest "Simpsonite" animasarja ühes osas, millele ta ise stsenaariumi kirjutas

  12. Hydrogen Energy Storage: Grid and Transportation Services (Technical Report)

    Energy Technology Data Exchange (ETDEWEB)

    Melaina, M. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Eichman, J. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2015-02-01

    Proceedings of an expert workshop convened by the U.S. Department of Energy and Industry Canada, and hosted by the National Renewable Energy Laboratory and the California Air Resources Board, May 14-15, 2014, in Sacramento, California, to address the topic of hydrogen energy storage (HES). HES systems provide multiple opportunities to increase the resilience and improve the economics of energy sup supply systems underlying the electric grid, gas pipeline systems, and transportation fuels. This is especially the case when considering particular social goals and market drivers, such as reducing carbon emissions, increasing reliability of supply, and reducing consumption of conventional petroleum fuels. This report compiles feedback collected during the workshop, which focused on policy and regulatory issues related to HES systems. Report sections include an introduction to HES pathways, market demand, and the "smart gas" concept; an overview of the workshop structure; and summary results from panel presentations and breakout groups.

  13. Ukraina president läks tülli ka uue valitsusega / Heiki Suurkask

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2008-01-01

    Ukraina peaminister Julia Tõmoshenko tahab anda riigiettevõtted erakätesse, president Viktor Jushtshenko on selle vastu. Valitsus tahtis tagandada riigivarafondi juhi, nähes temas erastamise pidurdajat, president keeldust teda ametist vabastamist

  14. Idiopathic hypereosinophilic syndrome associated with rheumatoid arthritis A case report

    Directory of Open Access Journals (Sweden)

    P. Quattrocchi

    2011-09-01

    Full Text Available The idiopathic hypereosinophilic sindrome (HES is a disease characterized by persistent blood eosinophilia (> 1500 eosinophils/mm3 > 6 months-in absence of other ethiologies for eosinophilia (parasitic, allergic, immunological or malignant diseases-associated with multiple organ involvement (heart, lung, central nervous system, skin, bone marrow, gastrointestinal tract. Reports on rheumatologic manifestations in patients with HES are very rare. In the case we report a typical rheumatoid arthritis developed in a 58-year-old woman with HES treated with glucocorticoids. Because of the marked glucocorticoids side effects shown by the patient(cushingoid habitus, hyperglycemia, we stopped this treatment and replaced it at first by methotrexate and later by cyclosporin, both of them associated with sulfasalazine. These drugs revealed very efficacious both on articular pathology and on the clinical and laboratory manifestations of HES. These data suggest that common pathogenetic mechanisms are likely acting in rheumatoid arthritis and idiopathic hypereosinophilic syndrome.

  15. The case of "A cat and dog in a well": motifs, folk inquiry and metafolkloristic interpretation (on Udmurtian folk beliefs) / Tatyana Minniyakhmetova

    Index Scriptorium Estoniae

    Minnijahmetova, Tatjana, 1961-

    2004-01-01

    Juhtumist ühes udmurdi külas Bashkiirias 1984. aastal, kui kahe perekonna kasutatavasse kaevu olid kukkunud surnud kass ja koer. Sellele järgnenud sündmustest, mida rahvauskumuste kohaselt seletati nõidusega

  16. Eesmärk on säästa inimeste elu / Jüri Pihl

    Index Scriptorium Estoniae

    Pihl, Jüri, 1954-

    2007-01-01

    Ilmunud ka: Vali Uudised, 9. nov. 2007, lk. 9; Virumaa Teataja, 13. nov. 2007, lk. 11; Meie Maa, 13. nov. 2007, lk. 2; Võrumaa Teataja, 13. nov. 2007, lk. 2; Koit, 13. nov. 2007, lk. 6; Sakala, 21. nov. 2007, lk. 2; Valgamaalane, 27. nov. 2007, lk. 2; Lõunaleht, 6. dets. 2007, lk. 4. Siseministeeriumi ja politsei tegevusest liiklusõnnetuste ja neis hukkuvate inimeste arvu vähendamiseks. Tabel ja diagramm: 2006. ja 2007. aasta 10 kuu jooksul liiklusõnnetustest hukkunud

  17. Ivar Tallo: presidendi erakorteris käinud peod mainet ei kahjusta / Ivar Tallo ; interv. Sigrid Laev

    Index Scriptorium Estoniae

    Tallo, Ivar, 1964-

    2006-01-01

    Noortepeod Kadrioru presidendilossis. Avaliku halduse spetsialist vastab küsimustele, kuidas jaguneb Kadrioru lossis era- ja avalik ruum, kas on õigustatud presidendi kantselei taotlus registreerida kõik, ka erakülalised ning kas juhtunu võib kahjustada presidendi mainet. Kaart, skeem: sissepääsud. Vt. samas. Presidendipaar palus vabandust ; Politsei kogub Kadrioru lastepidude kohta infot. Tallinna Vanalinna hariduskolleegiumi põhikooli juhataja Alar Saare kommentaar ; Garel Püüa. Kärmas: tegu ei ole poliitilise vandenõuga

  18. Probo Koala omanik kasutab Aafrikat mürgijäätmete prügilana / Kadri Ibrus

    Index Scriptorium Estoniae

    Ibrus, Kadri

    2006-01-01

    Välisajakirjanduse andmeil kasutab tankeri Probo Koala omanik, naftaärifirma Trafigura Aafrikat oma mürgiste jäätmete prügimäena, sest Aafrika sadamates saab neist jäätmetest lahti tunduvalt odavamalt kui Euroopas. Tankeriga seotud intsidendist Amsterdami sadamas. Omanikfirma varasem kohtuasi USA-s. Kaart: Mürgilaeva eksirännakud. Vt. samas: Pardal toodeti libabensiini; Politsei küsitles tankeri meeskonda; Eesti ootab jäätmekäitlusel EL-i abi; Tankeri meeskond ohus

  19. Techno-economic feasibility analysis of solar photovoltaic power generation for buildings

    International Nuclear Information System (INIS)

    Zhang, Xiongwen; Li, Menyu; Ge, Yuanfei; Li, Guojun

    2016-01-01

    Highlights: • A model for optimal component sizes of hybrid energy system (HES) is presented. • The techno-economic feasibility of PV for building in context of China is studied. • The use of PV reduces COE by 46% for customers in the commercial building. • The use of PV increases COE by 9.55% for customers in the residential building. - Abstract: The Building Added PV (BAPV) plays an important role for developing green buildings. This work conducts a techno-economic feasibility study of BAPV for commercial and residential building hybrid energy systems (HES). A component sizing model based on the optimal power dispatch simulations with the objective of minimum cost of energy (COE) is used to determine the component sizes of HES. The techno-economic performances of two HES composed of BAPV and batteries for residential and commercial buildings are investigated. The results show that the use of BAPV in the commercial building HES can reduce the electricity bill for customers owing to the government subsidies on PV as well as due to the similar characteristics of the load profile as to the solar radiation profile. However, due to temporal dislocation between the load and solar radiation patterns, the use of PV in the residential building HES may significantly increase the initial capital cost and replacement cost of battery, resulting in the COE of the residential building HES with BAPV even higher than the residential electricity price. The techno-economic performances of battery (e.g., the lifetime and capital cost) have more effect on the COE of the residential building HES than that of PV.

  20. Hesperidin as radioprotector against radiation-induced lung damage in rat: A histopathological study

    Directory of Open Access Journals (Sweden)

    Gholam Hassan Haddadi

    2017-01-01

    Full Text Available Reactive oxygen species (ROS are generated by ionizing radiation, and one of the organs commonly affected by ROS is the lung. Radiation-induced lung injury including pneumonia and lung fibrosis is a dose-limiting factor in radiotherapy (RT of patients with thorax irradiation. Administration of antioxidants has been proved to protect against ROS. The present study was aimed to assess the protective effect of hesperidin (HES against radiation-induced lung injury of male rats. Fifty rats were divided into three groups. G1: Received no HES and radiation (sham. G2: Underwent γ-irradiation to the thorax. G3: Received HES and underwent γ-irradiation. The rats were exposed to a single dose of 18 Gy using cobalt-60 unit and were administered HES (100 mg/kg for 7 days before irradiation. Histopathological analysis was performed 24 h and 8 weeks after RT. Histopathological results in 24 h showed radiation-induced inflammation and presence of more inflammatory cells as compared to G1 (P < 0.05. Administration of HES significantly decreased such an effect when compared to G2 (P < 0.05. Histopathological evaluation in 8 weeks showed a significant increase in mast cells, inflammation, inflammatory cells, alveolar thickness, vascular thickness, pulmonary edema, and fibrosis in G2 when compared to G1 (P < 0.05. HES significantly decreased inflammatory response, fibrosis, and mast cells when compared to G2 (P < 0.05. Administration of HES resulted in decreased radiation pneumonitis and radiation fibrosis in the lung tissue. Thus, the present study showed HES to be an efficient radioprotector against radiation-induced damage in the lung of tissue rats.

  1. Spatial planning for a green economy: National-level hydrologic ecosystem services priority areas for Gabon.

    Science.gov (United States)

    Goldstein, Joshua Howard; Tallis, Heather; Cole, Aaron; Schill, Steven; Martin, Erik; Heiner, Michael; Paiz, Marie-Claire; Aldous, Allison; Apse, Colin; Nickel, Barry

    2017-01-01

    Rapidly developing countries contain both the bulk of intact natural areas and biodiversity, and the greatest untapped natural resource stocks, placing them at the forefront of "green" economic development opportunities. However, most lack scientific tools to create development plans that account for biodiversity and ecosystem services, diminishing the real potential to be sustainable. Existing methods focus on biodiversity and carbon priority areas across large geographies (e.g., countries, states/provinces), leaving out essential services associated with water supplies, among others. These hydrologic ecosystem services (HES) are especially absent from methods applied at large geographies and in data-limited contexts. Here, we present a novel, spatially explicit, and relatively simple methodology to identify countrywide HES priority areas. We applied our methodology to the Gabonese Republic, a country undergoing a major economic transformation under a governmental commitment to balance conservation and development goals. We present the first national-scale maps of HES priority areas across Gabon for erosion control, nutrient retention, and groundwater recharge. Priority sub-watersheds covered 44% of the country's extent. Only 3% of the country was identified as a priority area for all HES simultaneously, highlighting the need to conserve different areas for each different hydrologic service. While spatial tradeoffs occur amongst HES, we identified synergies with two other conservation values, given that 66% of HES priority areas intersect regions of above average area-weighted (by sub-watersheds) total forest carbon stocks and 38% intersect with terrestrial national parks. Considering implications for development, we identified HES priority areas overlapping current or proposed major roads, forestry concessions, and active mining concessions, highlighting the need for proactive planning for avoidance areas and compensatory offsets to mitigate potential conflicts

  2. Follow-up of cerebral aneurysm embolization with hydrogel embolic system: Systematic review and meta-analysis.

    Science.gov (United States)

    Serafin, Zbigniew; Di Leo, Giovanni; Pałys, Alicja; Nowaczewska, Magdalena; Beuth, Wojciech; Sardanelli, Francesco

    2015-10-01

    Hydrogel embolic system (HES) for endovascular treatment of cerebral aneurysms was developed to reduce the risk of aneurysm recurrence and the rate of retreatment. The aim of this systematic review was to verify the efficacy of HES, also in comparison to bare platinum coils (BPC). A systematic literature search was performed by two independent reviewers for articles published until January 31st, 2013 on the follow-up recurrence rate of intracranial aneurysm embolization with hydrogel-coated coils. Thirteen articles on the use of HES for embolization of cerebral aneurysms were included in this study, for a total of 1683 embolized aneurysms. Significant heterogeneity was found regarding patient populations, methods of aneurysm coiling, follow-up schedules, and recurrence definitions. The pooled rate of initial complete aneurysm occlusion was 55% (95%CI, 38-70%); 59% (95%CI, 43-74%) in the subgroup of aneurysms treated with the use of HES. The pooled total recurrence rate was 19% (95%CI, 15-24%); 17% (95%CI, 14-22%) in the HES-treated subgroup. The pooled major recurrence rate was 12% (95%CI, 8-18%); 11% (95%CI, 7-16%) in the HES-treated subgroup. Comparing both types of coils, the pooled odds ratio for total recurrence was 0.63 (95%CI, 0.45-0.88; p=0.008) in favor of HES. Embolization of cerebral aneurysms using HES seems to result in a lower rate of recurrence than that obtained using BPC, which is in line with the only randomized controlled trial. High-quality prospective studies are needed to define specific indications for the use of hydrogel-coated coils. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  3. Acute kidney injury in critically burned patients resuscitated with a protocol that includes low doses of Hydroxyethyl Starch

    OpenAIRE

    Sánchez-Sánchez, M.; Garcia-de-Lorenzo, A.; Cachafeiro, L.; Herrero, E.; Asensio, MJ.; Agrifoglio, A.; Flores, E.; Estebanez, B.; Extremera, P.; Iglesias, C.; Martinez, J.R..

    2016-01-01

    Acute kidney injury (AKI) is an important complication in burn patients. Recently, it has been recommended that hydroxyethyl starch (HES) be avoided in burn patients because it increases the incidence of AKI. Our purpose was to study incidence of AKI in critically ill burn patients resuscitated with Ringer’s solution and supplements of HES. We conducted an observational study of 165 patients admitted to the critical care burn unit (with 30 ± 15% TBSA burned). The main outcome measures were in...

  4. Dynamic analysis of hybrid energy systems under flexible operation and variable renewable generation – Part II: Dynamic cost analysis

    International Nuclear Information System (INIS)

    Garcia, Humberto E.; Mohanty, Amit; Lin, Wen-Chiao; Cherry, Robert S.

    2013-01-01

    Dynamic analysis of HES (hybrid energy systems) under flexible operation and variable renewable generation is considered in this two-part communication to better understand various challenges and opportunities associated with the high system variability arising from the integration of renewable energy into the power grid. Advanced HES solutions are investigated in which multiple forms of energy commodities, such as electricity and chemical products, may be exchanged. In particular, a comparative dynamic cost analysis is conducted in this part two of the communication to determine best HES options. The cost function includes a set of metrics for computing fixed costs, such as fixed operations and maintenance and overnight capital costs, and also variable operational costs, such as cost of operational variability, variable operations and maintenance cost, and cost of environmental impact, together with revenues. Assuming natural gas, coal, and nuclear as primary heat sources, preliminary results identify the level of renewable penetration at which a given advanced HES option (e.g., a nuclear hybrid) becomes increasingly more economical than a traditional electricity-only generation solution. Conditions are also revealed under which carbon resources may be better utilized as carbon sources for chemical production rather than as combustion material for electricity generation. - Highlights: ► Dynamic analysis of HES to investigate challenges related to renewable penetration. ► Evaluation of dynamic synergies among HES constituents on system performance. ► Comparison of traditional versus advanced HES candidates. ► Dynamic cost analysis of HES candidates to investigate their economic viability. ► Identification of conditions under which an energy commodity may be best utilized

  5. Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products.

    Science.gov (United States)

    Zhu, Fenlu; Heditke, Sarah; Kurtzberg, Joanne; Waters-Pick, Barbara; Hari, Parameswaran; Margolis, David A; Keever-Taylor, Carolyn A

    2015-12-01

    Removing DMSO post-thaw results in: reduced infusion reactions, improved recovery and stability of viable CD34+ cells. Validated methods use 5%-8.3% Dextran 40 with 2.5%-4.2% HSA for this purpose. Recent shortages of clinical grade Dextran require identification of suitable alternatives. PBPC were used to compare a standard 2X wash medium of 5 parts 10% Dextran 40 in saline (DEX) with 1 part 25% HSA (8.3% DEX/ 4.2% HSA) with Hydroxyethyl Starch (HES)-based solutions. Cells in replicate bags were diluted with an equal volume of wash solution, equilibrated 5 minutes, the bag filled with wash medium, pelleted and the supernatant expressed. Bags were restored to the frozen volume in wash medium and tested by single platform flow cytometry and CFU. Total viability, viable TNC, MNC, and CD34+ cell recovery, and CD34+ cell viability were compared immediately post-thaw and after 90 minutes. 5.2% HES/4.2% HSA did not differ from our standard in CD34 recovery or viability. Due to concerns that high concentrations of HES could affect renal function we tested 0.6% HES/2.5% HSA resulting in significantly poorer CD34 recovery and viability. Results improved using 2.4% HES/4.2% HSA and when 0.6% HES/4.2%HSA was used no significant differences were seen. CFU assays confirmed no differences between the standard dextran arm and HES at 2.4% or 0.6% so long as HSA was at 4.2%. We conclude that HES from 0.6% to 5.2% with 4.2% HSA is a suitable substitute for Dextran 40 as a reconstitution/washing medium for PBPC products. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  6. IgG4-related disease and lymphocyte-variant hypereosinophilic syndrome: A comparative case series.

    Science.gov (United States)

    Carruthers, Mollie N; Park, Sujin; Slack, Graham W; Dalal, Bakul I; Skinnider, Brian F; Schaeffer, David F; Dutz, Jan P; Law, Joanna K; Donnellan, Fergal; Marquez, Vladimir; Seidman, Michael; Wong, Patrick C; Mattman, Andre; Chen, Luke Y C

    2017-04-01

    To compare the clinical and laboratory features of IgG4-related disease (IgG4-RD) and lymphocyte-variant hypereosinophilic syndrome (L-HES), two rare diseases that often present with lymphadenopathy, gastrointestinal symptoms, eosinophilia, and elevated immunoglobulins/IgE. Comparative case series of 31 patients with IgG4-RD and 13 patients with L-HES. Peripheral blood eosinophilia was present in eight of 31 patients with IgG4-RD compared to 13 of 13 patients with L-HES (median eosinophils 0.4 vs 7.0 giga/L, P=.001) and 12 of 20 patients with IgG4-RD had increased serum IgE compared to eight of 13 patients with L-HES, P=.930. Twenty-seven of 30 patients with IgG4-RD had elevated serum IgG4 compared to five of 12 patients with L-HES (median IgG4 9.6 g/L vs 0.80 g/L, P=.002). Flow cytometry demonstrated an aberrant T-cell phenotype in 7 of 23 patients with IgG4-RD and 13 of 13 patients with L-HES (PIgG4-RD vs 10 of 13 patients with L-HES (P=.143). Patients in both groups received corticosteroids as first-line therapy. For refractory disease in IgG4-RD, rituximab was the most common steroid-sparing agent, whereas in L-HES, it was pegylated interferon-α-2a. The overlapping features of these two diseases with divergent treatment options demonstrate the importance of familiarity with both entities to optimize diagnosis and treatment. © 2016 The Authors. European Journal of Haematology Published by John Wiley & Sons Ltd.

  7. Eighteen-Year Cryopreservation Does Not Negatively Affect the Pluripotency of Human Embryos: Evidence from Embryonic Stem Cell Derivation

    Science.gov (United States)

    Rungsiwiwut, Ruttachuk; Numchaisrika, Pranee; Ahnonkitpanit, Vichuda; Isarasena, Nipan; Virutamasen, Pramuan

    2012-01-01

    Abstract Human embryonic stem (hES) cells are considered to be a potential source for the therapy of human diseases, drug screening, and the study of developmental biology. In the present study, we successfully derived hES cell lines from blastocysts developed from frozen and fresh embryos. Seventeen- to eighteen-year-old frozen embryos were thawed, cultured to the blastocyst stage, and induced to form hES cells using human foreskin fibroblasts. The Chula2.hES cell line and the Chula4.hES and Chula5.hES cell lines were derived from blastocysts developed from frozen and fresh embryos, respectively. The cell lines expressed pluripotent markers, including alkaline phosphatase (AP), Oct3/4, stage-specific embryonic antigen (SSEA)-4, and tumor recognition antigen (TRA)-1-60 and TRA-1-81 as detected with immunocytochemistry. The real-time polymerase chain reaction (RT-PCR) results showed that the cell lines expressed pluripotent genes, including OCT3/4, SOX2, NANOG, UTF, LIN28, REX1, NODAL, and E-Cadherin. In addition, the telomerase activities of the cell lines were higher than in the fibroblast cells. Moreover, the cell lines differentiated into all three germ layers both in vitro and in vivo. The cell lines had distinct identities, as revealed with DNA fingerprinting, and maintained their normal karyotype after a long-term culture. This study is the first to report the successful derivation of hES cell lines in Thailand and that frozen embryos maintained their pluripotency similar to fresh embryos, as shown by the success of hES cell derivation, even after years of cryopreservation. Therefore, embryos from prolonged cryopreservation could be an alternative source for embryonic stem cell research. PMID:23514952

  8. EST Table: AV401797 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available AV401797 heS00172 10/09/28 94 %/235 aa ref|NP_001036831.1| saposin-related [Bombyx ...9/10 low homology 10/09/10 low homology 10/09/10 41 %/191 aa gi|91077504|ref|XP_966852.1| PREDICTED: similar to saposin isoform 1 [Tribolium castaneum] FS791050 heS0 ...

  9. Spatial planning for a green economy: National-level hydrologic ecosystem services priority areas for Gabon.

    Directory of Open Access Journals (Sweden)

    Joshua Howard Goldstein

    Full Text Available Rapidly developing countries contain both the bulk of intact natural areas and biodiversity, and the greatest untapped natural resource stocks, placing them at the forefront of "green" economic development opportunities. However, most lack scientific tools to create development plans that account for biodiversity and ecosystem services, diminishing the real potential to be sustainable. Existing methods focus on biodiversity and carbon priority areas across large geographies (e.g., countries, states/provinces, leaving out essential services associated with water supplies, among others. These hydrologic ecosystem services (HES are especially absent from methods applied at large geographies and in data-limited contexts. Here, we present a novel, spatially explicit, and relatively simple methodology to identify countrywide HES priority areas. We applied our methodology to the Gabonese Republic, a country undergoing a major economic transformation under a governmental commitment to balance conservation and development goals. We present the first national-scale maps of HES priority areas across Gabon for erosion control, nutrient retention, and groundwater recharge. Priority sub-watersheds covered 44% of the country's extent. Only 3% of the country was identified as a priority area for all HES simultaneously, highlighting the need to conserve different areas for each different hydrologic service. While spatial tradeoffs occur amongst HES, we identified synergies with two other conservation values, given that 66% of HES priority areas intersect regions of above average area-weighted (by sub-watersheds total forest carbon stocks and 38% intersect with terrestrial national parks. Considering implications for development, we identified HES priority areas overlapping current or proposed major roads, forestry concessions, and active mining concessions, highlighting the need for proactive planning for avoidance areas and compensatory offsets to mitigate

  10. Effects of hydroxyethyl starch in subgroups of patients with severe sepsis

    DEFF Research Database (Denmark)

    Müller, Rasmus G; Haase, Nicolai; Wetterslev, Jørn

    2013-01-01

    It has been speculated that certain subgroups of sepsis patients may benefit from treatment with hydroxyethyl starch (HES) 130/0.42, specifically in the earlier resuscitation of patients with more severely impaired circulation.......It has been speculated that certain subgroups of sepsis patients may benefit from treatment with hydroxyethyl starch (HES) 130/0.42, specifically in the earlier resuscitation of patients with more severely impaired circulation....

  11. Operations Optimization of Hybrid Energy Systems under Variable Markets

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Jun; Garcia, Humberto E.

    2016-07-01

    Hybrid energy systems (HES) have been proposed to be an important element to enable increasing penetration of clean energy. This paper investigates the operations flexibility of HES, and develops a methodology for operations optimization to maximize its economic value based on predicted renewable generation and market information. The proposed operations optimizer allows systematic control of energy conversion for maximal economic value, and is illustrated by numerical results.

  12. Health Education Specialists' Knowledge, Attitudes, and Perceptions of the Patient Protection and Affordable Care Act.

    Science.gov (United States)

    Strong, Jessica; Hanson, Carl L; Magnusson, Brianna; Neiger, Brad

    2016-03-01

    The changing landscape of health care as a result of the Patient Protection and Affordable Care Act (ACA) may provide new opportunities for health education specialists (HES). The purpose of this study was to survey HES in the United States on their knowledge and attitudes of the ACA and assess their perceptions of job growth under the law. A random sample of 220 (36% response rate) certified HES completed a 53-item cross sectional survey administered online through Qualtrics. Findings were compared to public opinion on health care reform. HES are highly favorable of the law (70%) compared to the general public (23%). A total of 85% of respondents were able to list a provision of the ACA, and most (81%) thought the ACA would be successful at increasing insured Americans. Over half (64.6%) believe job opportunities will increase. Those who viewed the law favorably were significantly more likely to score better on a knowledge scale related to the ACA. HES understand publicized provisions but are uncertain about common myths and specific provisions related to Title IV, "Prevention of Chronic Disease and Improving Public Health." Directed and continuing education to HES regarding the ACA is warranted. © 2015 Society for Public Health Education.

  13. Efficient differentiation of human embryonic stem cells to definitive endoderm.

    Science.gov (United States)

    D'Amour, Kevin A; Agulnick, Alan D; Eliazer, Susan; Kelly, Olivia G; Kroon, Evert; Baetge, Emmanuel E

    2005-12-01

    The potential of human embryonic stem (hES) cells to differentiate into cell types of a variety of organs has generated much excitement over the possible use of hES cells in therapeutic applications. Of great interest are organs derived from definitive endoderm, such as the pancreas. We have focused on directing hES cells to the definitive endoderm lineage as this step is a prerequisite for efficient differentiation to mature endoderm derivatives. Differentiation of hES cells in the presence of activin A and low serum produced cultures consisting of up to 80% definitive endoderm cells. This population was further enriched to near homogeneity using the cell-surface receptor CXCR4. The process of definitive endoderm formation in differentiating hES cell cultures includes an apparent epithelial-to-mesenchymal transition and a dynamic gene expression profile that are reminiscent of vertebrate gastrulation. These findings may facilitate the use of hES cells for therapeutic purposes and as in vitro models of development.

  14. Optimising methods of red cell sedimentation from cord blood to maximise nucleated cell recovery prior to cryopreservation.

    Science.gov (United States)

    Madkaikar, M; Gupta, M; Ghosh, K; Swaminathan, S; Sonawane, L; Mohanty, D

    2007-01-01

    Human cord blood is now an established source of stem cells for haematopoietic reconstitution. Red blood cell (RBC) depletion is required to reduce the cord blood unit volume for commercial banking. Red cell sedimentation using hydroxy ethyl starch (HES) is a standard procedure in most cord blood banks. However, while standardising the procedure for cord blood banking, a significant loss of nucleated cells (NC) may be encountered during standard HES sedimentation protocols. This study compares four procedures for cord blood processing to obtain optimal yield of nucleated cells. Gelatin, dextran, 6% HES and 6% HES with an equal volume of phosphate-buffered saline (PBS) were compared for RBC depletion and NC recovery. Dilution of the cord blood unit with an equal volume of PBS prior to sedimentation with HES resulted in maximum NC recovery (99% [99.5 +/- 1.3%]). Although standard procedures using 6% HES are well established in Western countries, they may not be applicable in India, as a variety of factors that can affect RBC sedimentation (e.g., iron deficiency, hypoalbuminaemia, thalassaemia trait, etc.) may reduce RBC sedimentation and thus reduce NC recovery. While diluting cord blood with an equal volume of PBS is a simple method to improve the NC recovery, it does involve an additional processing step.

  15. Servo-controlled hind-limb electrical stimulation for short-term arterial pressure control.

    Science.gov (United States)

    Kawada, Toru; Shimizu, Shuji; Yamamoto, Hiromi; Shishido, Toshiaki; Kamiya, Atsunori; Miyamoto, Tadayoshi; Sunagawa, Kenji; Sugimachi, Masaru

    2009-05-01

    Autonomic neural intervention is a promising tool for modulating the circulatory system thereby treating some cardiovascular diseases. In 8 pentobarbital-anesthetized cats, it was examined whether the arterial pressure (AP) could be controlled by acupuncture-like hind-limb electrical stimulation (HES). With a 0.5-ms pulse width, HES monotonically reduced AP as the stimulus current increased from 1 to 5 mA, suggesting that the stimulus current could be a primary control variable. In contrast, the depressor effect of HES showed a nadir approximately 10 Hz in the frequency range between 1 and 100 Hz. Dynamic characteristics of the AP response to HES approximated a second-order low-pass filter with dead time (gain: -10.2 +/- 1.6 mmHg/mA, natural frequency: 0.040 +/- 0.004 Hz, damping ratio 1.80 +/- 0.24, dead time: 1.38 +/- 0.13 s, mean +/- SE). Based on these dynamic characteristics, a servo-controlled HES system was developed. When a target AP value was set at 20 mmHg below the baseline AP, the time required for the AP response to reach 90% of the target level was 38 +/- 10 s. The steady-state error between the measured and target AP values was 1.3 +/- 0.1 mmHg. Autonomic neural intervention by acupuncture-like HES might provide an additional modality to quantitatively control the circulatory system.

  16. Estrogen-dependent downregulation of hairy and enhancer of split homolog-1 gene expression in breast cancer cells is mediated via a 3' distal element.

    Science.gov (United States)

    Müller, Patrick; Merrell, Kenneth W; Crofts, Justin D; Rönnlund, Caroline; Lin, Chin-Yo; Gustafsson, Jan-Ake; Ström, Anders

    2009-03-01

    Regulation of hairy and enhancer of split homologue-1 (HES-1) by estradiol and all-trans retinoic acid affects proliferation of human breast cancer cells. Here, we identify and characterize cis-regulatory elements involved in HES-1 regulation. In the distal 5' promoter of the HES-1 gene, we found a retinoic acid response element and in the distal 3' region, an estrogen receptor alpha(ER)alpha binding site. The ERalpha binding site, composed of an estrogen response element (ERE) and an ERE half-site, is important for both ERalpha binding and transcriptional regulation. Chromatin immunoprecipitation assays revealed that ERalpha is recruited to the ERE and associates with the HES-1 promoter. We also show recruitment of nuclear receptor co-regulators to the ERE in response to estradiol, followed by a decrease in histone acetylation and RNA polymerase II docking in the HES-1 promoter region. Our findings are consistent with a novel type of repressive estrogen response element in the distal 3' region of the HES-1 gene.

  17. Kuidas eesti keele õppijad ja kasutajad lause verbi ajavormi sobivust hindavad?

    Directory of Open Access Journals (Sweden)

    Diana Maisla

    2011-05-01

    Full Text Available Verbi ajavormide kasutamine on üks grammatika valdkondadest, mis pakub jätkuvalt huvi teise keele omandamise uurijatele. Artiklis on vaatluse all grammatilise sobivuse hindamise testi tulemused, milles võrreldakse 96 vene ja 100 eesti emakeelega üliõpilase valikuid ülesandes, kus tuli hinnata oleviku ja kolme mineviku ajavormi sobivust eestikeelsesse lausesse skaalal sobib täpselt / sobib samuti / ei sobi. Kogutud andmeid on töödeldud SPSS-programmi abil ning statistiliste testide tulemuste põhjal selgitatud välja erinevused keelejuhtide rühmade vahel. Olulised erinevused kahe rühma vahel ilmnesid täis- ja enneminevikulise verbi sobivusele antud hinnangute vahel, kuid oleviku ja lihtmineviku sobivust on vene keelejuhid hinnanud eesti keelejuhtidega suhteliselt sarnaselt. http://dx.doi.org/10.5128/ERYa7.06

  18. Retrospective evaluation of the effects of administration of tetrastarch (hydroxyethyl starch 130/0.4) on plasma creatinine concentration in dogs (2010-2013): 201 dogs.

    Science.gov (United States)

    Yozova, Ivayla D; Howard, Judith; Adamik, Katja-Nicole

    2016-07-01

    To determine changes in creatinine concentrations following the administration of 6% tetrastarch (hydroxyethyl starch [HES] 130/0.4) compared to crystalloids (CRYSs) in critically ill dogs. Retrospective case series (2010-2013). University teaching hospital. Two hundred and one dogs admitted to the intensive care unit with initial plasma creatinine concentrations not exceeding laboratory reference intervals (52-117 μmol/L [0.6-1.3 mg/dL]) and receiving either CRYSs alone (CRYS group, n = 115) or HES with or without CRYSs (HES group, n = 86) for at least 24 hours. None. Creatinine concentrations at admission to the intensive care unit (T0), and 2-13 days (T1) and 2-12 weeks (T2) after initiation of fluid therapy were analyzed. Creatinine concentrations were analyzed as absolute values and as the maximum percentage change from T0 to T1 (T1max%) and from T0 to T2 (T2max%), respectively. Creatinine concentrations were available for 192 dogs during T1 and 37 dogs during T2. The median cumulative dose of HES was 86 mL/kg (range, 12-336 mL/kg). No difference was detected between the groups for age, gender, body weight, and length of hospitalization. Outcome was significantly different between the HES (66% survived) and the CRYS (87% survived) groups (P = 0.014). No significant difference was detected between groups for creatinine concentrations at T0, T1, T2, T1max%, or T2max%. No significant difference was detected between the groups for T1max% creatinine in dogs subclassified as having systemic inflammatory response syndrome or sepsis. HES administration in this canine population did not result in increased creatinine concentrations compared to administration of CRYSs. Further studies are needed to establish the safety of HES in critically ill dogs. © Veterinary Emergency and Critical Care Society 2016.

  19. Private patients in NHS hospitals: comparison of two sources of information.

    Science.gov (United States)

    Williams, B T; Pearson, J

    1999-03-01

    The use of National Health Service (NHS) hospitals to treat private patients is debatable on the grounds of equity of access. Hospital Episodes Statistics (HES) annual reports are the only routine source of information on the scale of this activity. The accuracy of the information is doubted. This enquiry tested the completeness of HES data against information obtained directly from private patient unit managers. Managers of the 71 pay bed units in NHS hospitals in England were asked to supply from local registers and accounts the numbers of in-patients and day cases admitted in 1995-1996. Their reports were matched with the numbers of first consultant episodes for private in-patients and day cases shown for those hospitals in the HES data file for that year. Of the 71 units 62 responded; 53 of these gave usable data. The 53 included, and 18 excluded from the comparison, matched on median and range of bed numbers. Managers identified 16 per cent more total admissions than did HES, 62,572 against 54,131; 13 per cent more in-patient admissions, 39,776 against 35,319; and 21 per cent more day cases, 22,796 against 18,812. More total admissions were reported by managers of 38 pay bed units than were recorded in HES, fewer by 12, and equal numbers by three. Similar sized discrepancies were noted for in-patient admissions and day cases. Reasons for the under-reporting of private patients in HES included the use of separate patient administration systems for private patients with a failure to feed data to HES, and the omission of some provider units altogether by a minority of trusts from the returns made to the Department of Health. Overall, HES underestimates the amount of private patient activity reported directly by NHS hospitals. No method of validating private patient data is currently available. An amendment to an existing statistical return would provide a check on numbers. Central guidance on the inclusion of private patient activity in data transmitted by

  20. Self-contained induction of neurons from human embryonic stem cells.

    Directory of Open Access Journals (Sweden)

    Tsuyoshi Okuno

    Full Text Available BACKGROUND: Neurons and glial cells can be efficiently induced from mouse embryonic stem (ES cells in a conditioned medium collected from rat primary-cultured astrocytes (P-ACM. However, the use of rodent primary cells for clinical applications may be hampered by limited supply and risk of contamination with xeno-proteins. METHODOLOGY/PRINCIPAL FINDINGS: We have developed an alternative method for unimpeded production of human neurons under xeno-free conditions. Initially, neural stem cells in sphere-like clusters were induced from human ES (hES cells after being cultured in P-ACM under free-floating conditions. The resultant neural stem cells could circumferentially proliferate under subsequent adhesive culture, and selectively differentiate into neurons or astrocytes by changing the medium to P-ACM or G5, respectively. These hES cell-derived neurons and astrocytes could procure functions similar to those of primary cells. Interestingly, a conditioned medium obtained from the hES cell-derived astrocytes (ES-ACM could successfully be used to substitute P-ACM for induction of neurons. Neurons made by this method could survive in mice brain after xeno-transplantation. CONCLUSION/SIGNIFICANCE: By inducing astrocytes from hES cells in a chemically defined medium, we could produce human neurons without the use of P-ACM. This self-serving method provides an unlimited source of human neural cells and may facilitate clinical applications of hES cells for neurological diseases.

  1. Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4+/CD8+ T Cell Ratio

    Directory of Open Access Journals (Sweden)

    Abdelaziz S. A. Abuelsaad

    2014-01-01

    Full Text Available Background. Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of human diseases. Hesperidin (HES has been reported to exert antioxidant and anti-inflammatory activities. Objectives. The aim of this study was to investigate the potential effect of HES treatment on inflammatory response induced by A. hydrophila infection in murine. Methods. A. hydrophila-infected mice were treated with HES at 250 mg/kg b.wt./week for 4 consecutive weeks. Phagocytosis, reactive oxygen species production, CD4+/CD8+ T cell ratio, and CD14 expression on intestinal infiltrating monocytes were evaluated. The expression of E-selectin and intercellular adhesion molecule 1 on stimulated HUVECs and RAW macrophage was evaluated. Results. Percentage of CD4+ T cells in the intestinal tissues of infected treated mice was highly significantly increased; however, phagocytic index, ROS production, CD8+ T cells percentage, and CD14 expression on monocytes were significantly reduced. On the other hand, HES significantly inhibited A-LPS- and A-ECP-induced E-selectin and ICAM-1 expression on HUVECs and ICAM-1 expression on RAW macrophage. Conclusion. Present data indicated that HES has a potential role in the suppression of inflammatory response induced by A. hydrophila toxins through downmodulation of ROS production and CD14 and adhesion molecules expression, as well as increase of CD4+/CD8+ cell ratio.

  2. A Combined Fuzzy-AHP and Fuzzy-GRA Methodology for Hydrogen Energy Storage Method Selection in Turkey

    Directory of Open Access Journals (Sweden)

    Aytac Yildiz

    2013-06-01

    Full Text Available In this paper, we aim to select the most appropriate Hydrogen Energy Storage (HES method for Turkey from among the alternatives of tank, metal hydride and chemical storage, which are determined based on expert opinions and literature review. Thus, we propose a Buckley extension based fuzzy Analytical Hierarchical Process (Fuzzy-AHP and linear normalization based fuzzy Grey Relational Analysis (Fuzzy-GRA combined Multi Criteria Decision Making (MCDM methodology. This combined approach can be applied to a complex decision process, which often makes sense with subjective data or vague information; and used to solve to solve HES selection problem with different defuzzification methods. The proposed approach is unique both in the HES literature and the MCDM literature.

  3. Generation of human embryonic stem cells from abnormal blastocyst diagnosed with albinism.

    Science.gov (United States)

    Sun, Yi; Zhou, Xiaoying; Chen, Jing; Du, Juan; Lu, Guangxiu; Lin, Ge; Ouyang, Qi

    2016-11-01

    Human embryonic stem cell (hESC) line chHES-478 was derived from abnormal blastocyst diagnosed with albinism after preimplantation genetic diagnosis (PGD) treatment. DNA sequencing analysis confirmed that chHES-478 cell line carried a compound heterozygous mutation, c.896G>A(p.Arg299His) and c.929_930insC(p.Pro310Glnfs*9), of TYR gene. Characteristic tests proved that the chHES-478 cell line presented typical markers of pluripotency and had the capability to form the three germ layers both in vitro and in vivo. Copyright © 2016 Michael Boutros, German Cancer Research Center, Heidelberg, Germany. Published by Elsevier B.V. All rights reserved.

  4. Multi-Objective Optimization Design for a Hybrid Energy System Using the Genetic Algorithm

    Directory of Open Access Journals (Sweden)

    Myeong Jin Ko

    2015-04-01

    Full Text Available To secure a stable energy supply and bring renewable energy to buildings within a reasonable cost range, a hybrid energy system (HES that integrates both fossil fuel energy systems (FFESs and new and renewable energy systems (NRESs needs to be designed and applied. This paper presents a methodology to optimize a HES consisting of three types of NRESs and six types of FFESs while simultaneously minimizing life cycle cost (LCC, maximizing penetration of renewable energy and minimizing annual greenhouse gas (GHG emissions. An elitist non-dominated sorting genetic algorithm is utilized for multi-objective optimization. As an example, we have designed the optimal configuration and sizing for a HES in an elementary school. The evolution of Pareto-optimal solutions according to the variation in the economic, technical and environmental objective functions through generations is discussed. The pair wise trade-offs among the three objectives are also examined.

  5. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells.

    Science.gov (United States)

    D'Amour, Kevin A; Bang, Anne G; Eliazer, Susan; Kelly, Olivia G; Agulnick, Alan D; Smart, Nora G; Moorman, Mark A; Kroon, Evert; Carpenter, Melissa K; Baetge, Emmanuel E

    2006-11-01

    Of paramount importance for the development of cell therapies to treat diabetes is the production of sufficient numbers of pancreatic endocrine cells that function similarly to primary islets. We have developed a differentiation process that converts human embryonic stem (hES) cells to endocrine cells capable of synthesizing the pancreatic hormones insulin, glucagon, somatostatin, pancreatic polypeptide and ghrelin. This process mimics in vivo pancreatic organogenesis by directing cells through stages resembling definitive endoderm, gut-tube endoderm, pancreatic endoderm and endocrine precursor--en route to cells that express endocrine hormones. The hES cell-derived insulin-expressing cells have an insulin content approaching that of adult islets. Similar to fetal beta-cells, they release C-peptide in response to multiple secretory stimuli, but only minimally to glucose. Production of these hES cell-derived endocrine cells may represent a critical step in the development of a renewable source of cells for diabetes cell therapy.

  6. Eosinophilic Pleural Effusion: A Rare Manifestation of Hypereosinophilic Syndrome

    Directory of Open Access Journals (Sweden)

    Ndubuisi C. Okafor

    2009-01-01

    Full Text Available Several causes of eosinophilic pleural effusions have been described with malignancy being the commonest cause. Hypereosinophilic syndrome (HES is a rare disease and very few cases have been reported of HES presenting as eosinophilic pleural effusion (EPE. We report a case of a 26-year-old male who presented with shortness of breath. He had bilateral pleural effusions, generalized lymphadenopathy, splenomegaly, and leukocytosis with marked peripheral blood eosinophilia. The pleural fluid was exudative, with 25%–30% eosinophilis, and absence of neoplastic cells. Hypereosinophilic syndrome was diagnosed after other causes of eosinophilia were excluded. He continued to be dyspneic with persistent accumulation of eosinophilic pleural fluid, even after his peripheral eosinophil count had normalized in response to treatment. This patient represents a very unusual presentation of HES with dyspnea and pleural effusions and demonstrates that treatment based on response of peripheral eosinophil counts, as is currently recommended, may not always be clinically adequate.

  7. Development of the hard and soft constraints based optimisation model for unit sizing of the hybrid renewable energy system designed for microgrid applications

    Science.gov (United States)

    Sundaramoorthy, Kumaravel

    2017-02-01

    The hybrid energy systems (HESs) based electricity generation system has become a more attractive solution for rural electrification nowadays. Economically feasible and technically reliable HESs are solidly based on an optimisation stage. This article discusses about the optimal unit sizing model with the objective function to minimise the total cost of the HES. Three typical rural sites from southern part of India have been selected for the application of the developed optimisation methodology. Feasibility studies and sensitivity analysis on the optimal HES are discussed elaborately in this article. A comparison has been carried out with the Hybrid Optimization Model for Electric Renewable optimisation model for three sites. The optimal HES is found with less total net present rate and rate of energy compared with the existing method

  8. Nuclear Hybrid Energy Systems - Regional Studies. West Texas and Northeastern Arizona

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Humberto E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Chen, Jun [Idaho National Lab. (INL), Idaho Falls, ID (United States); Kim, Jong S. [Idaho National Lab. (INL), Idaho Falls, ID (United States); McKellar, Michael G. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Deason, Wesley R. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Vilim, Richard B. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bragg-Sitton, Shannon M. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Boardman, Richard D. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-04-01

    The primary objective of this study is to conduct a preliminary dynamic analysis of two realistic hybrid energy systems (HES) including a nuclear reactor as the main baseload heat generator (denoted as nuclear HES or nuclear hybrid energy systems [NHES]) and to assess the local (e.g., HES owners) and system (e.g., the electric grid) benefits attainable by the application of NHES in scenarios with multiple commodity production and high penetration of renewable energy. It is performed for regional cases - not generic examples - based on available resources, existing infrastructure, and markets within the selected regions. This study also briefly addresses the computational capabilities developed to conduct such analyses, reviews technical gaps, and suggests some research paths forward.

  9. Nuclear Hybrid Energy Systems Regional Studies: West Texas & Northeastern Arizona

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Humberto E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Chen, Jun [Idaho National Lab. (INL), Idaho Falls, ID (United States); Kim, Jong Suk [Idaho National Lab. (INL), Idaho Falls, ID (United States); McKellar, Michael George [Idaho National Lab. (INL), Idaho Falls, ID (United States); Deason, Wesley R [Idaho National Lab. (INL), Idaho Falls, ID (United States); Vilim, Richard B. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bragg-Sitton, Shannon M. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Boardman, Richard D. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-04-01

    The primary objective of this study is to conduct a preliminary dynamic analysis of two realistic hybrid energy systems (HES) including a nuclear reactor as the main baseload heat generator (denoted as nuclear HES or nuclear hybrid energy systems [[NHES]) and to assess the local (e.g., HES owners) and system (e.g., the electric grid) benefits attainable by the application of NHES in scenarios with multiple commodity production and high penetration of renewable energy. It is performed for regional cases not generic examples based on available resources, existing infrastructure, and markets within the selected regions. This study also briefly addresses the computational capabilities developed to conduct such analyses, reviews technical gaps, and suggests some research paths forward.

  10. Therapeutic potential of adult stem cells

    DEFF Research Database (Denmark)

    Serakinci, Nedime; Keith, W. Nicol

    2006-01-01

    is the necessity to be able to identify, select, expand and manipulate cells outside the body. Recent advances in adult stem cell technologies and basic biology have accelerated therapeutic opportunities aimed at eventual clinical applications. Adult stem cells with the ability to differentiate down multiple...... lineages are an attractive alternative to human embryonic stem cells (hES) in regenerative medicine. In many countries, present legislation surrounding hES cells makes their use problematic, and indeed the origin of hES cells may represent a controversial issue for many communities. However, adult stem...... cells are not subject to these issues. This review will therefore focus on adult stem cells. Based on their extensive differentiation potential and, in some cases, the relative ease of their isolation, adult stem cells are appropriate for clinical development. Recently, several observations suggest...

  11. Clinical management of the hypereosinophilic syndromes.

    Science.gov (United States)

    Cogan, Elie; Roufosse, Florence

    2012-06-01

    Hypereosinophilic syndromes (HESs) are rare disorders characterized by marked hypereosinophilia that is directly responsible for organ damage or dysfunction. Different pathogenic mechanisms have been discovered in patient subgroups leading to the characterization of myeloproliferative and lymphocytic disease variants. In the updated terminology, idiopathic HES is now restricted to patients with HES of undetermined etiology. The practical clinical approach of patients with the different HES variants is reviewed herein, focusing on specific diagnostic tools and therapeutic options. Corticosteroids, hydroxyurea and IFN-α remain the classical agents for treatment of most patients with HESs. The specific role of therapeutic compounds that have become available more recently, namely, tyrosine kinase inhibitors and IL-5 antagonists, is discussed.

  12. An integrated optimization approach for a hybrid energy system in electric vehicles

    International Nuclear Information System (INIS)

    Hung, Yi-Hsuan; Wu, Chien-Hsun

    2012-01-01

    Highlights: ► Second-order control-oriented dynamics for a battery/supercapacitor EV is modeled. ► Multiple for-loop programming and global searchwith constraints are main design principles of integrated optimization algorithm (IOA). ► Optimal hybridization is derived based on maximizing energy storage capacity. ► Optimal energy management in three EV operation modes is searched based on minimizing total consumed power. ► Simulation results prove that 6+% of total energy is saved by the IOA method. -- Abstract: This paper develops a simple but innovative integrated optimization approach (IOA) for deriving the best solutions of component sizing and control strategies of a hybrid energy system (HES) which consists of a lithium battery and a supercapacitor module. To implement IOA, a multiple for-loop structure with a preset cost function is needed to globally calculate the best hybridization and energy management of the HES. For system hybridization, the optimal size ratio is evaluated by maximizing the HES energy stored capacity at various costs. For energy management, the optimal power distribution combined with a three-mode rule-based strategy is searched to minimize the total consumed energy. Combining above two for-loop structures and giving a time-dependent test scenario, the IOA is derived by minimizing the accumulated HES power. Simulation results show that 6% of the total HES energy can be saved in the IOA case compared with the original system in two driving cycles: ECE and UDDS, and two vehicle weights, respectively. It proves that the IOA effectively derives the maximum energy storage capacity and the minimum energy consumption of the HES at the same time. Experimental verification will be carried out in the near future.

  13. Serum biomarkers are similar in Churg-Strauss syndrome and hypereosinophilic syndrome.

    Science.gov (United States)

    Khoury, P; Zagallo, P; Talar-Williams, C; Santos, C S; Dinerman, E; Holland, N C; Klion, A D

    2012-09-01

    Churg-Strauss syndrome (CSS) and hypereosinophilic syndrome (HES) overlap considerably in clinical presentation. A reliable means of distinguishing between these groups of patients is needed, especially in the setting of glucocorticoid therapy. A retrospective chart review of 276 adult subjects referred for evaluation of eosinophilia > 1500/μl was performed, and subjects with a documented secondary cause of eosinophilia or a PDGFR -positive myeloproliferative neoplasm were excluded. The remaining subjects were assessed for the presence of American College of Rheumatology (ACR) criteria. Laboratory and clinical parameters were compared between subjects with biopsy-proven vasculitis (CSS; n = 8), ≥4 ACR criteria (probable CSS; n = 21), HES with asthma and/or sinusitis without other CSS-defining criteria (HESwAS; n = 20), HES without asthma or sinusitis (HES; n = 18), and normal controls (n = 8). Serum biomarkers reported to be associated with CSS were measured using standard techniques. There were no differences between the subjects with definite or probable CSS or HES with respect to age, gender, or maintenance steroid dose. Serum CCL17, IL-8, and eotaxin levels were significantly increased in eosinophilic subjects as compared to normal controls, but were similar between the eosinophilic groups. Serum CCL17 correlated with eosinophil count (P < 0.0001, r = 0.73), but not with prednisone dose. In patients with a history of asthma and sinusitis, distinguishing between ANCA-negative CSS and PDGFR-negative HES is difficult because of significant overlap in clinical presentation and biomarker profiles. Published 2012. This article is a U.S. Government work and is in the public domain in the USA.

  14. Effect of Hydroxyethyl Starch Solution on Incidence of Acute Kidney Injury in Patients Suffering from Cerebral Vasospasm Following Aneurysmal Subarachnoid Hemorrhage.

    Science.gov (United States)

    Kieninger, Martin; Unbekannt, Daniel; Schneiker, André; Sinner, Barbara; Bele, Sylvia; Prasser, Christopher

    2017-02-01

    The application of third-generation hydroxyethyl starch (HES) solutions in critically ill patients suffering from aneurysmal subarachnoid hemorrhage (aSAH) was often part of the treatment of delayed cerebral ischemia (DCI). However, there is increasing evidence showing a correlation between the application of HES and the incidence of acute kidney injury (AKI). In a single-center retrospective analysis including 81 patients without a preexisting renal disorder suffering from aSAH who had received higher volumes of 6 % HES 130/0.4 due to standard treatment of DCI, the incidence of AKI during intensive care unit (ICU) stay was recorded using AKIN criteria. Furthermore, the course of serum creatinine after discharge from ICU was observed. 6 % HES 130/0.4 was given over a period of 12.9 ± 7.1 days resulting in a cumulative dose of 12543.2 ± 7743.6 mL. Four patients (4.9 %) fulfilled AKIN criteria stage 1 during ICU stay. In two of these patients, serum creatinine was within normal range again on day of discharge. Five patients showed elevated levels of serum creatinine within 1 to 22 months after hospitalization. A correlation between the amount of HES given and the incidence of AKI could not be found. The application of 6 % HES 130/0.4 did not lead to an elevated incidence of AKI in patients without an elevated baseline serum creatinine. However, there is still a lack of high-level evidence as prospective randomized trials are missing yet.

  15. Hyperbranched polyglycerol as a colloid in cold organ preservation solutions.

    Directory of Open Access Journals (Sweden)

    Sihai Gao

    Full Text Available Hydroxyethyl starch (HES is a common colloid in organ preservation solutions, such as in University of Wisconsin (UW solution, for preventing graft interstitial edema and cell swelling during cold preservation of donor organs. However, HES has undesirable characteristics, such as high viscosity, causing kidney injury and aggregation of erythrocytes. Hyperbranched polyglycerol (HPG is a branched compact polymer that has low intrinsic viscosity. This study investigated HPG (MW-0.5 to 119 kDa as a potential alternative to HES for cold organ preservation. HPG was synthesized by ring-opening multibranching polymerization of glycidol. Both rat myocardiocytes and human endothelial cells were used as an in vitro model, and heart transplantation in mice as an in vivo model. Tissue damage or cell death was determined by both biochemical and histological analysis. HPG polymers were more compact with relatively low polydispersity index than HES in UW solution. Cold preservation of mouse hearts ex vivo in HPG solutions reduced organ damage in comparison to those in HES-based UW solution. Both size and concentration of HPGs contributed to the protection of the donor organs; 1 kDa HPG at 3 wt% solution was superior to HES-based UW solution and other HPGs. Heart transplants preserved with HPG solution (1 kDa, 3% as compared with those with UW solution had a better functional recovery, less tissue injury and neutrophil infiltration in syngeneic recipients, and survived longer in allogeneic recipients. In cultured myocardiocytes or endothelial cells, significantly more cells survived after cold preservation with the HPG solution than those with the UW solution, which was positively correlated with the maintenance of intracellular adenosine triphosphate and cell membrane fluidity. In conclusion, HPG solution significantly enhanced the protection of hearts or cells during cold storage, suggesting that HPG is a promising colloid for the cold storage of donor organs

  16. Influence of Two Different Types of Cardioplegia on Hemodilution During and After Cardiopulmonary Bypass, Postoperative Chest-Drainage Bleeding and Consumption of Donor Blood Products

    Directory of Open Access Journals (Sweden)

    Mirsad Kacila

    2006-08-01

    Full Text Available The aim of this study is to compare the effects of colloidal cardioplegia and blood cardioplegia in patients who underwent cardiac surgical procedures with cardiopulmonary bypass, and to evaluate their influence on hemodilution, bleeding and consumption of donor blood productsin a retrospective clinical study. 100 male patients who underwent cardiac surgical procedure were divided into two groups: 50 patients were administered intermittent normotherm or mild hypotherm (34 degrees C Calafiore blood cardioplegia with potassium chloride 14,9%; 50 patients were administered one initial doses of cold Kirsch - solution followed from intermittent cold colloidal cardioplegia using hydroxyethyl starch (HES 450/0,7. Hemoglobin values after the first dose of cardioplegia were significantly lower in the HES-group than in the Calafiore- group. After the first dose of cardioplegia platelets count was lower in the HES-group than in the Calafiore-group. Hemoglobin and hematocrit values 24h postoperative were lower in the HES-group than in the Calafiore-group. There was no difference in chest-drainagebleeding 12h and 24h postoperative between the groups. The consumption of donor erythrocyte concentrate and fresh frozen plasma was significantly higher in the HES-than in the Calafiore- group. The choice of either colloidal or blood cardioplegia does not influence the postoperative chest-drainage bleeding. The results suggest that high molecular colloidal cardioplegia with HES-solution is associated with higher hemodilution during and after cardiopulmonary bypass and significantly increases the consumption of donor blood products.

  17. Stuart Energy's experiences in developing 'Hydrogen Energy Station' infrastructure

    International Nuclear Information System (INIS)

    Crilly, B.

    2004-01-01

    'Full text:' With over 50 years experience, Stuart Energy is the global leader in the development, manufacture and integration of multi-use hydrogen infrastructure products that use the Company's proprietary IMET hydrogen generation water electrolysis technology. Stuart Energy offers its customers the power of hydrogen through its integrated Hydrogen Energy Station (HES) that provides clean, secure and distributed hydrogen. The HES can be comprised of five modules: hydrogen generation, compression, storage, fuel dispensing and / or power generation. This paper discusses Stuart Energy's involvement with over 10 stations installed in recent years throughout North America, Asia and Europe while examining the economic and environmental benefits of these systems. (author)

  18. Occupational inequalities in health expectancies in France in the early 2000s: Unequal chances of reaching and living retirement in good health

    Directory of Open Access Journals (Sweden)

    Emmanuelle Cambois

    2011-08-01

    Full Text Available Increasing life expectancy (LE raises expectations for social participation at later ages. We computed health expectancies (HE to assess the (unequal chances of social/work participation after age 50 in the context of France in 2003. We considered five HEs, covering various health situations which can jeopardize participation, and focused on both older ages and the pre-retirement period. HEs reveal large inequalities for both sexes in the chances of remaining healthy after retirement, and also of reaching retirement age in good health and without disability, especially in low-qualified occupations. These results challenge the policy expectation of an overall increase in social participation at later ages.

  19. A randomized prospective analysis of alteration of hemostatic function in patients receiving tranexamic acid and hydroxyethyl starch (130/0.4 undergoing off pump coronary artery bypass surgery

    Directory of Open Access Journals (Sweden)

    Murali Chakravarthy

    2012-01-01

    Full Text Available Postoperative hemorrhagic complications is still one of the major problems in cardiac surgeries. It may be caused by surgical issues, coagulopathy caused by the side effects of the intravenous fluids administered to produce plasma volume expansion such as hydroxyl ethyl starch (HES. In order to thwart this hemorrhagic issue, few agents are available. Fibrinolytic inhibitors like tranexamic acid (TA may be effective modes to promote blood conservation; but the possible complications of thrombosis of coronary artery graft, precludes their generous use in coronary artery bypass graft surgery. The issue is a balance between agents that promote coagulation and those which oppose it. Therefore, in this study we have assessed the effects of concomitant use of HES and TA. Thromboelastogram (TEG was used to assess the effect of the combination of HES and TA. With ethical committee approval and patient′s consent, 100 consecutive patients were recruited for the study. Surgical and anesthetic techniques were standardized. Patients fulfilling our inclusion criteria were randomly allocated into 4 groups of 25 each. The patients in group A received 20 ml/kg of HES (130/0.4, 10 mg/kg of T.A over 30 minutes followed by infusion of 1 mg/kg/hr over the next 12 hrs. The patients in group B received Ringer′s lactate + TA at same dose. The patients in the Group C received 20 ml/kg of HES. Group D patients received RL. Fluid therapy was goal directed. Total blood loss was assessed. Reaction time (r, α angle, maximum amplitude (MA values of TEG were assessed at baseline, 12, 36 hrs. The possible perioperative myocardial infraction (MI was assessed by electrocardiogram (ECG and troponin T values at the baseline, postoperative day 1. Duration on ventilator, length of stay (LOS in the intensive care unit (ICU were also assessed. The demographical profile was similar among the groups. Use of HES increased blood loss significantly (P < 0.05. Concomitant use of TA

  20. Lihula lahing / Igor Gräzin

    Index Scriptorium Estoniae

    Gräzin, Igor, 1952-

    2004-01-01

    Ilmunud ka: Meie Maa, 15. sept. 2004, lk. 2; Põhjarannik, 15. sept. 2004, lk. 2; Severnoje Poberezhje, 15. sept. 2004, lk. 2; Koit, 16. sept. 2004, lk. 6; Pärnu Postimees, 17. sept. 2004, lk. 11; Hiiu Leht, 17. sept. 2004, lk. 2; Harjumaa, 17. sept. 2004, lk. 7; Lääne Elu, 18. sept. 2004, lk. 2; Valgamaalane, 21. sept. 2004, lk. 2; Nädaline, 21. sept. 2004, lk. 4; Võrumaa Teataja, 21. sept. 2004, lk. 2; Sakala, 23. sept. 2004, lk. 2; Paremad Uudised, nr. 8, sept., 2004, lk. 7. Lihula mälestusmärgi mahavõtmisest, politsei, siseministri käitumisest sündmuste ajal

  1. Individual patient data meta-analysis of hydroxyethyl starch 130/0.4-0.42 versus crystalloid for fluid resuscitation in patients with severe sepsis

    DEFF Research Database (Denmark)

    Hammond, Naomi E; Haase, Nicolai; Billot, Laurent

    2014-01-01

    BACKGROUND: The Crystalloid versus Hydroxyethyl Starch Trial (CHEST) and the Scandinavian Starch in Severe Sepsis/ Septic Shock (6S) trial reported that 6% hydroxyethyl starch (HES) is associated with increased use of renal replacement therapy and death in critically ill patients. Data collection...

  2. Tšehhi võimud tahavad endale USA raketikilpi / Krister Paris

    Index Scriptorium Estoniae

    Paris, Krister, 1977-

    2006-01-01

    USA rajab 2011. aastaks raketirünnakute tõrje süsteemi. Raketikilbi Euroopa-keskuse asukohale kandideerivad Tšehhi ja Poola, samas on suur osa tshehhe USA baasi rajamise vastu. Skeem: USA tähesõdade programm. Lisa: Peaminister vaid nime poolest

  3. Acute toxicity of the chloroacetanilide herbicide butachlor and its ...

    African Journals Online (AJOL)

    DR C.D NWANI

    2013-01-30

    Jan 30, 2013 ... as wheat, barley, cotton, vegetables and peanuts. It reac- hes aquatic environment due to the proximities of the agricultural country sides to water places. ... and affect the thyroid and sex steroid hormones in Zebra fish (Chang et al., 2011). The fish Tilapia zillii is com- monly found in all African countries and ...

  4. Endoparasites of the subantarctic fur seal Arctocephalus tropicalis ...

    African Journals Online (AJOL)

    1989-03-03

    Mar 3, 1989 ... 77°35'E) and Gough Island (40"20'S / 09°54'W) amongst others in the Southern Ocean, with vagrants from an unknown source turning up on the South. African coastline (Bester 1980; Shaughnessy & Ross. 1980; Hes & Roux 1983). Very little is known of the parasites of this species since only a few of the ...

  5. Milleks meile Chomsky? / Joonas Kiik

    Index Scriptorium Estoniae

    Kiik, Joonas

    2006-01-01

    Autor vaatleb Noam Chomsky käsitlusi demokraatia levitamisest Iraagi näitel ja ohust inimkonna tulevikule tähesõdade näitel. Autori hinnangul on Eesti valitsuse puhul tegemist sisuliselt eliidi valitsusega, mis tegutseb valdavalt iseenda huvidest lähtudes. Rahva enamus ei usalda poliitikuid, kuid lepib olukorraga

  6. Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy

    Directory of Open Access Journals (Sweden)

    Xinxin Han

    2017-01-01

    Full Text Available Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.

  7. Muusikaliteatri pidu moeka komöödiaga

    Index Scriptorium Estoniae

    2008-01-01

    Novembris kümnendat sünnipäeva tähistav Muusikaliteater toob Tallinna Linnahallis lavale Cole Porteri muusikalise komöödia "Nii on meil moes!" (esietendus 14. nov., lavastaja Andrus Vaarik, ühes peaosas Kristi Roosmaa)

  8. I tõ, Brjus / Katja Tarhanova

    Index Scriptorium Estoniae

    Tarhanova, Katja

    2006-01-01

    Ameerika mängufilmid "Lucky Number Slevin" : režissöör Paul McGuigan ja "16 kvartalit" ("16 Blocks") : režissöör Richard Donner. Mõlemas mängib Bruce Willis, ühes mõrvarit, teises politseinikku

  9. Privacy preserving cloud computation using Domingo-Ferrer scheme

    Directory of Open Access Journals (Sweden)

    Abdulatif Alabdulatif

    2016-01-01

    Full Text Available Homomorphic encryption system (HES schemes are anticipated to play a significant role in cloud-based applications. Moving to cloud-based storage and analytic services securely are two of the most important advantages of HES. Several HES schemes have been recently proposed. However, the majority of them either have limited capabilities or are impractical in real-world applications. Various HES schemes provide the ability to perform computations for statistical analysis (e.g. average, mean and variance on encrypted data. Domingo-Ferrer is one scheme that has privacy homomorphism properties to perform the basic mathematical operations (addition, subtraction and multiplication in a convenient and secure way. However, it works only in the positive numbers’ range which is considered as a limitation because several applications require both positive and negative ranges in which to work, especially those that have to implement analytical services in cloud computing. In this paper, we extend Domingo-Ferrer’s scheme to be able to perform arithmetic operations for both positive and negative numbers. We also propose using a lightweight data aggregation function to compute both maximum and minimum values of the aggregated data that works for both positive and negative numbers.

  10. Elamise tornist fantaasiamaastikuni

    Index Scriptorium Estoniae

    2001-01-01

    Vitra Disainimuuseumi avanäitus taani disainer Verner Pantoni loomingust: ühes tükis täisplastist tooli looja, mööbel-skulptuurid, elukeskkonna mõtestamine, kolmemõõtmelise ruumi täielik ekspluateerimine

  11. Planeetide rivistus pakub sajandi vaatemängu / Villu Päärt

    Index Scriptorium Estoniae

    Päärt, Villu, 1972-

    2002-01-01

    Viis planeeti - Merkuur, Veenus, Marss, Saturn ja Jupiter koonduvad 20. aprillist kuuks ajaks õhtutaevas rivvi, mida näeb uuesti alles saja aasta pärast. Skeem : Meie päikesesüsteemi planeedid; Planeedid ühes reas; Kuu varjutab Saturni

  12. Email authentication using symmetric and asymmetric key algorithm encryption

    Science.gov (United States)

    Halim, Mohamad Azhar Abdul; Wen, Chuah Chai; Rahmi, Isredza; Abdullah, Nurul Azma; Rahman, Nurul Hidayah Ab.

    2017-10-01

    Protection of sensitive or classified data from unauthorized access, hackers and other personals is virtue. Storage of data is done in devices such as USB, external hard disk, laptops, I-Pad or at cloud. Cloud computing presents with both ups and downs. However, storing information elsewhere increases risk of being attacked by hackers. Besides, the risk of losing the device or being stolen is increased in case of storage in portable devices. There are array of mediums of communications and even emails used to send data or information but these technologies come along with severe weaknesses such as absence of confidentiality where the message sent can be altered and sent to the recipient. No proofs are shown to the recipient that the message received is altered. The recipient would not find out unless he or she checks with the sender. Without encrypted of data or message, sniffing tools and software can be used to hack and read the information since it is in plaintext. Therefore, an electronic mail authentication is proposed, namely Hybrid Encryption System (HES). The security of HES is protected using asymmetric and symmetric key algorithms. The asymmetric algorithm is RSA and symmetric algorithm is Advance Encryption Standard. With the combination for both algorithms in the HES may provide the confidentiality and authenticity to the electronic documents send from the sender to the recipient. In a nutshell, the HES will help users to protect their valuable documentation and data from illegal third party user.

  13. Linnumustriga laulupeosokkidest on saanud Suva vabriku hitt-toode / Erik Müürsepp

    Index Scriptorium Estoniae

    Müürsepp, Erik

    2009-01-01

    Suurest huvist AS Suva toodetud laulu- ja tantsupeo "ÜhesHingamine" temaatikaga sokkide ja põlvikute vastu. 25. laulu- ja 18. tantsupeo kunstniku Liina Tepandi ja AS Suva disaineri Leila Tamme kujundatud sokkide ja põlvikute mudelite ja värvide valikust

  14. Clausewitzi sõjateooria ja infooperatsioonid / William M Darley

    Index Scriptorium Estoniae

    Darley, William M

    2006-01-01

    Infooperatsioonidest Carl von Clausewitzi teooria kontekstis ning infooperatsioonide rollist kogu konfliktispektri ulatuses. Skeemid: Clausewitzi mudel poliitiliste eesmärkide saavutamiseks konflikti abil; Absoluutne vägivald ühes, puhas poliitika teises äärmuses; Operatsioonid paigutatuna "sõja skaalal"

  15. Toxcast Profiling in a Human Stem Cell Assay for Developmental Toxicity (SOT)

    Science.gov (United States)

    We correlated the ToxCast library in a metabolic biomarker-based in vitro assay (Stemina devTOXqP) utilizing human embryonic stem (hES) cells (H9 line). This assay identifies the concentration of a chemical that disrupts cellular metabolism in a manner indicative of teratogenic...

  16. Leukemia inhibitory factor (LIF) enhances MAP2 + and HUC/D + neurons and influences neurite extension during differentiation of neural progenitors derived from human embryonic stem cells.

    Science.gov (United States)

    Leukemia Inhibitory Factor (L1F), a member of the Interleukin 6 cytokine family, has a role in differentiation of Human Neural Progenitor (hNP) cells in vitro. hNP cells, derived from Human Embryonic Stem (hES) cells, have an unlimited capacity for self-renewal in monolayer cultu...

  17. Acute kidney injury in critically burned patients resuscitated with a protocol that includes low doses of Hydroxyethyl Starch.

    Science.gov (United States)

    Sánchez-Sánchez, M; Garcia-de-Lorenzo, A; Cachafeiro, L; Herrero, E; Asensio, M J; Agrifoglio, A; Flores, E; Estebanez, B; Extremera, P; Iglesias, C; Martinez, J R

    2016-09-30

    Acute kidney injury (AKI) is an important complication in burn patients. Recently, it has been recommended that hydroxyethyl starch (HES) be avoided in burn patients because it increases the incidence of AKI. Our purpose was to study incidence of AKI in critically ill burn patients resuscitated with Ringer's solution and supplements of HES. We conducted an observational study of 165 patients admitted to the critical care burn unit (with 30 ± 15% TBSA burned). The main outcome measures were incidence of AKI, contributions of colloids and crystalloids, various severity scores, comorbidities, complications and mortality. According to the RIFLE criteria, 10 (6.1%) patients presented with Risk, 11 (6.7%) presented with Injury and 11 (6.7%) presented with Failure. According to the AKIN criteria, 9.7% presented stage I, 3% stage II and 10.3% stage III. Replacement therapy (RRT) was performed in 15 patients (9.1%), but in 6 of them RRT was employed in the final stages of multi-organ failure. The incidence of AKI in severe burn patients is high according to the RIFLE or AKIN criteria and these patients experience more complications and higher mortality. Our study suggests that the use of HES in low doses in the burn resuscitation phase does not cause more AKI than resuscitation without HES, but further evaluation is required. Further studies should be conducted.

  18. 75 FR 6658 - Science Advisory Board Staff Office; Notification of a Public Teleconference of the Health...

    Science.gov (United States)

    2010-02-10

    ... Prospective Benefit-Cost Study of the Clean Air Act. DATES: The public teleconference will be held on Tuesday... Act Amendments, EPA conducts periodic studies to assess benefits and costs of the EPA's regulatory... evaluate the costs and benefits of EPA Clean Air programs for years 1990-2020. The Council HES met on...

  19. Tarbijalepingute kohtualluvuse probleemid Brüsseli I määruses: milliste ohtudega peavad arvestama oma kaupu või teenuseid piiriüleselt pakkuvad ettevõtjad? / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2014-01-01

    Millal võib ühes liikmesriigis elav tarbija, kes on ostnud kauba või saanud teenuse teises liikmesriigis asuvalt ettevõtjalt, esitada ettevõtja vastu hagi oma elukohariigi kohtusse. Euroopa Kohtu lahenditest kohtuasjades Alpenhof, Mühlleitner ja Emrek

  20. Concordia jäi elektrita / Sigrid Laev

    Index Scriptorium Estoniae

    Laev, Sigrid

    2003-01-01

    Concordia ülikooli haldaja keeras ülikooli ühes majas elektri välja, õppejõud ei saa oma arvutitele ligi ning pole ka internetti. Õppetöö siiski jätkub. Hoone haldaja ootab selgust, kes teenuste eest maksab. Concordia loodab leida olukorrale lahenduse investori tulekuga

  1. Lapse ülalpidamise hind / Ene-Margit Tiit

    Index Scriptorium Estoniae

    Tiit, Ene-Margit, 1934-

    2005-01-01

    Tutvustav artikkel sotsiaalministeeriumi tellimusel läbiviidud uuringust, mille eesmärgiks oli välja töötada lapse kõikidest vajadustest lähtuv ühtne metoodika lapse ülalpidamiskulude arvutamiseks ühes kuus. Tabelid. Diagrammid. Vt. ka lk. 15-16: Kaplan, Signe. Metoodika kasutamisvõimalusi

  2. Cancer recording in patients with type 2 diabetes in primary care and hospital admission data

    Directory of Open Access Journals (Sweden)

    Rachael Williams

    2017-04-01

    A good level of concordance and low level of misclassification of cancer exist between CPRD primary care data and HES. The value of linking these data for establishing cancer outcomes lies more in the complimentary variables held than in reducing misclassification.

  3. 500-tonniste muldseintega elamu / Ene Läkk, Helen Urbanik

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2000-01-01

    Arhitekt Rick Joy projekteeritud arst John Palmeri villa USAs Arizona osariigis, Santa Catalina mäestiku jalamil. Hoone ühes osas asuvad perekonna eluruumid, teine on avatud ruum. Pressitud muldseinad ja liblikatiiva kujulised terasest katusesiilud jäljendavad ümbritseva maastiku vorme. Suuri seina- ja aknapindu katab raamimata klaas.

  4. Eesti kasiinofirma sattus Leedus skandaali keskele / Toomas Sildam, Rasmus Kagge

    Index Scriptorium Estoniae

    Sildam, Toomas, 1961-

    2006-01-01

    Leedu julgeolekuamet konfiskeeris ajalehe Laisvas Laikrastise kogu 8.septembri tiraazhi, väidetavalt kirjutati ajalehes julgeolekuameti poolt pealtkuulatud telefonikõnedest, millest ühes olevat Eesti suurärimees, Olympic Casino omanik Armin Karu nõudnud oma Vilniuse esindajatelt kokkusaamist temalt raha saanud Leedu sotsiaaldemokraatliku partei liidri Arturas Paulauskasega

  5. 75 FR 6235 - Self-Regulatory Organizations; NASDAQ OMX BX, Inc.; Notice of Filing and Immediate Effectiveness...

    Science.gov (United States)

    2010-02-08

    ... security is trading above $200 or above a calculated index value of 200 shall be based on the price at the close of trading on the Expiration Friday prior to being added to the Penny Pilot Program. Based on... Corp. HBC HSBC Holdings PLC. WFR MEMC Electronic Materials Inc. HES Hess Corp. WFT Weatherford...

  6. Patterns of intravenous fluid resuscitation use in adult intensive care patients between 2007 and 2014

    DEFF Research Database (Denmark)

    Hammond, Naomi E; Taylor, Colman; Finfer, Simon

    2017-01-01

    BACKGROUND: In 2007, the Saline versus Albumin Fluid Evaluation-Translation of Research Into Practice Study (SAFE-TRIPS) reported that 0.9% sodium chloride (saline) and hydroxyethyl starch (HES) were the most commonly used resuscitation fluids in intensive care unit (ICU) patients. Evidence has e...

  7. Minimalistlikud muinasjutud / Veiko Märka

    Index Scriptorium Estoniae

    Märka, Veiko, 1964-

    2007-01-01

    Sisu: Feodaalkord Lapimaal ; Taimetoitlase õudusunenägu ; Savisaare pilt ; Mis mulle meenub, nähes lepatriinut? ; Viagra võluvägi ; Uskumatu loomaporno ; Tunne oma kodumaad ; Tunne oma kodumaad 2. ; Üleskutse Polüneesia puuviljakasvatajatele ; EELK peapiiskopi maja ; Stalingradi lahing ; Tark mees ; Teine Arnold ; Kolmas Arnold

  8. Tshuzhoi Tanel Padar / Dmitri Babitshenko

    Index Scriptorium Estoniae

    Babitshenko, Dmitri

    2007-01-01

    Saksamaalt pärit, Eesti ülikoolides filmiteooria ja -ajaloo loenguid pidav ja filmi "Ring tee" valmimist režissöörina juhtinud Dirk Hoyeri mängufilm esilinastub 27. apr. kinos "Sõprus". Praeguseks on film ümber nimetatud "Võõraks", ühes peaosadest astub üles muusik Tanel Padar

  9. Veneetsias lööb laineid kuuba kirjaniku eluloofilm / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    2000-01-01

    57. Veneetsia filmifestivalil tõusis üheks favoriidiks Julian Schnabeli mängufilm "Enne õhtu saabumist" ("Before Night Falls") : Ameerika Ühendriigid 2000. Riho Undi nukufilmi "Samueli Internet" näidati edukalt ühes alaprogrammis, mis läheb edasi Milanosse ja Rooma

  10. Disease cell models - their use in industry TEDD workshop in Sion on 27 March 2014.

    Science.gov (United States)

    Graf-Hausner, Ursula; Heinzelmann, Elsbeth

    2014-01-01

    On 27, March 2014, experts met at the first TEDD Workshop 2014, held at the HES-SO Valais/Wallis in Sion, to present innovative cell models for industrial applications. This was the first time that a TEDD event had been organized in French-speaking Switzerland and it offered local network partners an opportunity to showcase their research activities.

  11. Uusimmigrantide laste integreerumine Eesti kooli / Maris Tuulik, Maia Muldma

    Index Scriptorium Estoniae

    Tuulik, Maris

    2008-01-01

    Analüüsitakse uusimmigrantõpilaste õpetamisega seotud peamisi raskusi ja probleeme. Ankeetküsitlus on läbi viidud 2007.a. veebruaris ühes Tallinna gümnaasiumis, millel on nelja-aastane kogemus uusimmigrantidest laste õpetamisel

  12. Hypereosinophilic syndrome: Clinical, laboratory, and imaging manifestations in patients with hepatic involvement

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Gi Beom; Lee, Jong Min; Sung, Yeong Soon; Kang, Duk Sik [Kyungpook Natioanl University College of Medicine, Daegu (Korea, Republic of); Kim, Ok Hwoa [Dongkang general Hospital, Ulsan (Korea, Republic of)

    1993-07-15

    The hypereosinophilic syndrome (HES) commonly involves liver and spleen but only a few literature has reported the imaging features. In this article, we present the imaging features of the liver and spleen in HES patients together with clinical and laboratory features. This study included 5 HES patients with hepatic involvement. Extensive laboratory tests including multiple hematologic, serologic, parasitological, and immunologic examinations were performed. Imaging studies included CT, ultrasound (US)of upper abdomen and hepatosplenic scintigraphy. All patients were periodically examined by laboratory and imaging studies for 4 to 24 months. The common clinical presentations were weakness, mild fever, and dry cough. All patients revealed leukocytosis with eosinophilia of 40 to 80% and benign eosinophilic hyperplasia of the bone marrow. The percutaneous biopsy of the hepatic focal lesions performed in 2 patients showed numerous benigin eosinophilic infiltrates and one of them revealed combined calibration necrosis of hepatocytes. All cases revealed hepatomegaly with multiple focal lesions on at least on of CT, US, or scintigraphy. These findings completely disappeared in 2 to 6 months following medication of corticosteroid or antihistamines. The HES involved the liver and CT, US, or scintigraphic studies showed hepatic multifocal lesions with hepatomegaly. Differential diagnosis of these findings should include metastatic disease, lymphoma, leukemia, candidiasis or other opportunistic infections.

  13. Hypereosinophilic syndrome: Clinical, laboratory, and imaging manifestations in patients with hepatic involvement

    International Nuclear Information System (INIS)

    Kim, Gi Beom; Lee, Jong Min; Sung, Yeong Soon; Kang, Duk Sik; Kim, Ok Hwoa

    1993-01-01

    The hypereosinophilic syndrome (HES) commonly involves liver and spleen but only a few literature has reported the imaging features. In this article, we present the imaging features of the liver and spleen in HES patients together with clinical and laboratory features. This study included 5 HES patients with hepatic involvement. Extensive laboratory tests including multiple hematologic, serologic, parasitological, and immunologic examinations were performed. Imaging studies included CT, ultrasound (US)of upper abdomen and hepatosplenic scintigraphy. All patients were periodically examined by laboratory and imaging studies for 4 to 24 months. The common clinical presentations were weakness, mild fever, and dry cough. All patients revealed leukocytosis with eosinophilia of 40 to 80% and benign eosinophilic hyperplasia of the bone marrow. The percutaneous biopsy of the hepatic focal lesions performed in 2 patients showed numerous benigin eosinophilic infiltrates and one of them revealed combined calibration necrosis of hepatocytes. All cases revealed hepatomegaly with multiple focal lesions on at least on of CT, US, or scintigraphy. These findings completely disappeared in 2 to 6 months following medication of corticosteroid or antihistamines. The HES involved the liver and CT, US, or scintigraphic studies showed hepatic multifocal lesions with hepatomegaly. Differential diagnosis of these findings should include metastatic disease, lymphoma, leukemia, candidiasis or other opportunistic infections

  14. Visible light assisted photodecolorization of eosin-Y in aqueous solution using hesperidin modified TiO2 nanoparticles

    Science.gov (United States)

    Vignesh, K.; Suganthi, A.; Rajarajan, M.; Sakthivadivel, R.

    2012-03-01

    Hesperidin a flavanoid, modified TiO2 nanoparticles (Hes-TiO2) was synthesized to improve the visible light driven photocatalytic performance of TiO2. The synthesized nanoparticles were characterized by UV-visible diffuse reflectance spectroscopy (UV-vis-DRS), FT-IR, powder X-ray diffraction (XRD) and scanning electron microscopy (SEM). The photocatalytic activity of Hes-TiO2 was investigated based on the decolorization of eosin-Y under visible light irradiation. Hes-TiO2 showed high efficiency for the decolorization of eosin-Y. The influences of various reaction parameters like effect of pH, catalyst dosage and initial dye concentration on the photocatalytic efficiency were investigated. The adsorption of eosin-Y on Hes-TiO2 was found favorable by the Langmuir approach. The removal percentage of chemical oxygen demand (COD) was determined to evaluate the mineralization of eosin-Y during photodecolorization. Based on the intermediates obtained in the GC-MS spectroscopic technique, a probable degradation mechanism has been proposed.

  15. Visible light assisted photodecolorization of eosin-Y in aqueous solution using hesperidin modified TiO2 nanoparticles

    International Nuclear Information System (INIS)

    Vignesh, K.; Suganthi, A.; Rajarajan, M.; Sakthivadivel, R.

    2012-01-01

    Hesperidin a flavanoid, modified TiO 2 nanoparticles (Hes-TiO 2 ) was synthesized to improve the visible light driven photocatalytic performance of TiO 2 . The synthesized nanoparticles were characterized by UV-visible diffuse reflectance spectroscopy (UV-vis-DRS), FT-IR, powder X-ray diffraction (XRD) and scanning electron microscopy (SEM). The photocatalytic activity of Hes-TiO 2 was investigated based on the decolorization of eosin-Y under visible light irradiation. Hes-TiO 2 showed high efficiency for the decolorization of eosin-Y. The influences of various reaction parameters like effect of pH, catalyst dosage and initial dye concentration on the photocatalytic efficiency were investigated. The adsorption of eosin-Y on Hes-TiO 2 was found favorable by the Langmuir approach. The removal percentage of chemical oxygen demand (COD) was determined to evaluate the mineralization of eosin-Y during photodecolorization. Based on the intermediates obtained in the GC-MS spectroscopic technique, a probable degradation mechanism has been proposed.

  16. Visible light assisted photodecolorization of eosin-Y in aqueous solution using hesperidin modified TiO{sub 2} nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Vignesh, K. [P.G. and Research Department of Chemistry, Thiagarajar College, Madurai, Tamilnadu 625009 (India); Suganthi, A., E-mail: suganthiphd09@gmail.com [P.G. and Research Department of Chemistry, Thiagarajar College, Madurai, Tamilnadu 625009 (India); Rajarajan, M., E-mail: rajarajan_1962@yahoo.com [Department of Chemistry, C.P.A. College, Bodinayakanur, Tamilnadu 626513 (India); Sakthivadivel, R. [P.G. and Research Department of Chemistry, Thiagarajar College, Madurai, Tamilnadu 625009 (India)

    2012-03-01

    Hesperidin a flavanoid, modified TiO{sub 2} nanoparticles (Hes-TiO{sub 2}) was synthesized to improve the visible light driven photocatalytic performance of TiO{sub 2}. The synthesized nanoparticles were characterized by UV-visible diffuse reflectance spectroscopy (UV-vis-DRS), FT-IR, powder X-ray diffraction (XRD) and scanning electron microscopy (SEM). The photocatalytic activity of Hes-TiO{sub 2} was investigated based on the decolorization of eosin-Y under visible light irradiation. Hes-TiO{sub 2} showed high efficiency for the decolorization of eosin-Y. The influences of various reaction parameters like effect of pH, catalyst dosage and initial dye concentration on the photocatalytic efficiency were investigated. The adsorption of eosin-Y on Hes-TiO{sub 2} was found favorable by the Langmuir approach. The removal percentage of chemical oxygen demand (COD) was determined to evaluate the mineralization of eosin-Y during photodecolorization. Based on the intermediates obtained in the GC-MS spectroscopic technique, a probable degradation mechanism has been proposed.

  17. Country's Competitiveness and Sustainability in the Context of the Higher Education System Reforms

    Science.gov (United States)

    Jermolajeva, Elita; Aleksejeva, Ludmila

    2013-01-01

    The accumulation of knowledge and its use have become important factors that promote economic development as they contribute to a country's competitiveness in the global economy. The basic significance of research is obtained by defining new approaches in the organisation, function and efficiency of the higher education system (HES) by emphasising…

  18. Hydroxyethyl starch 130/0.4 versus modified fluid gelatin for volume expansion in cardiac surgery patients: the effects on perioperative bleeding and transfusion needs

    NARCIS (Netherlands)

    van der Linden, Philippe J.; de Hert, Stefan G.; Deraedt, Dirk; Cromheecke, Stefanie; de Decker, Koen; de Paep, Rudi; Rodrigus, Inez; Daper, Anne; Trenchant, Anne

    2005-01-01

    In this prospective, randomized, open controlled study we compared the effects on net red blood cell loss of 6% hydroxyethyl starch 130/0.4 (HES: n = 64) and 3% modified fluid gelatin (GEL: n = 68) administered for intravascular volume management in patients undergoing coronary surgery. Blood losses

  19. Nordea - investeering tulevikku / Alvar Jõgeva

    Index Scriptorium Estoniae

    Jõgeva, Alvar

    2004-01-01

    Autor analüüsib Põhja- ja Baltimaade juhtiva finantskontserni Nordea senist käekäiku, nähes selles üsna stabiilset pikaajalist investeeringut ning prognoosib aktsia hinna kasvu tulevikus. Diagramm: Nordea aktsia alustas tõusutrendi; Kasumile ennustatakse jätkuvat kasvu. Vt. samas: Carnegie soovitab aktsiat osta

  20. Иэна Макюэна обвинили в плагиате

    Index Scriptorium Estoniae

    2006-01-01

    Ian McEwanit süüdistatakse plagiaadis: 2006. a. surnud kirjaniku Lucilla Andrewsi agendid väidavad, et McEwan kasutas romaanis "Atonement" ("Lepitus") Andrewsi mälestusi töötamisest meditsiiniõena ühes Londoni haiglas Teise maailmasõja alguses

  1. Risk assessment as part of internal auditing in the government institutions of the Estonian Republic / Krisi Pungas

    Index Scriptorium Estoniae

    Pungas, Krisi

    2003-01-01

    Ülevaade sisekontrolli mõistest, eesmärkidest ja teostamisest Eesti Vabariigi valitsusasutustes. Sisekontrolli ülesandeks on välja selgitada kõige efektiivsem tee võimalike riskide juhtimiseks ühes organisatsioonis või tegevusvaldkonnas. Skeem

  2. The influence of a balanced volume replacement concept on inflammation, endothelial activation, and kidney integrity in elderly cardiac surgery patients.

    Science.gov (United States)

    Boldt, Joachim; Suttner, Stephan; Brosch, Christian; Lehmann, Andreas; Röhm, Kerstin; Mengistu, Andinet

    2009-03-01

    A balanced fluid replacement strategy appears to be promising for correcting hypovolemia. The benefits of a balanced fluid replacement regimen were studied in elderly cardiac surgery patients. In a randomized clinical trial, 50 patients aged >75 years undergoing cardiac surgery received a balanced 6% HES 130/0.42 plus a balanced crystalloid solution (n = 25) or a non-balanced HES in saline plus saline solution (n = 25) to keep pulmonary capillary wedge pressure/central venous pressure between 12-14 mmHg. Acid-base status, inflammation, endothelial activation (soluble intercellular adhesion molecule-1, kidney integrity (kidney-specific proteins glutathione transferase-alpha; neutrophil gelatinase-associated lipocalin) were studied after induction of anesthesia, 5 h after surgery, 1 and 2 days thereafter. Serum creatinine (sCr) was measured approximately 60 days after discharge. A total of 2,750 +/- 640 mL of balanced and 2,820 +/- 550 mL of unbalanced HES were given until the second POD. Base excess (BE) was significantly reduced in the unbalanced (from +1.21 +/- 0.3 to -4.39 +/- 1.0 mmol L(-1) 5 h after surgery; P volume replacement strategy including a balanced HES and a balanced crystalloid solution resulted in moderate beneficial effects on acid-base status, inflammation, endothelial activation, and kidney integrity compared to a conventional unbalanced volume replacement regimen.

  3. Computerized system for hospital engineering service management

    International Nuclear Information System (INIS)

    Centeno, C A; Gonzalez, E A; Cagnolo, F J; Olmos, C E

    2007-01-01

    When a Hospital Engineering Service (HES) is implemented within a health care environment, the idea is to improve service conditions and costs as well as to provide timely responses to equipment preventive maintenance and infrastructure requirements. An HES must, within the shortest possible period of time, meet the above requirements at the cost necessary to provide the service quality sought. In many cases there is a lack of minimal materials and staff who are qualified to attain the objectives that have been set. Therefore, external assistance becomes necessary. In this context, actions are often taken which, because they are not recorded, cannot be assessed in order to evaluate the HES. Since all action taken is appraised from the purely economic point of view, in the final analysis the contributions from staff remain invisible. This situation works against the possibility of quantifying the convenience of possessing an internal HES. The software support system we have developed here is oriented toward providing all the necessary data to address this issue

  4. Pärnumaa "Kultuuripärl" on Bruno Sõmeri / Silja Joon

    Index Scriptorium Estoniae

    Joon, Silja, 1966-

    2006-01-01

    28. dets. Pärnu kontserdimajas toimunud Pärnumaa kultuuri- ja sporditegijate aastalõpu galaõhtul "Tähesära 2006" pälvisid teisete seas preemiad ka lauluõpetaja Anne Uusna, laulukonkursi "Sügisulg" korraldaja Helen Erastus, koorijuht Toomas Voll, rahvatantsurühma Kajakas juht Rita Mändla ja kunstnik Toomas Kuusing

  5. Saksa filmilootusega tänavaelust ja hullusest / Robert Stadlober ; interv. Janno Zõbin

    Index Scriptorium Estoniae

    Stadlober, Robert

    2002-01-01

    Pimedate Ööde Filmifestivalil näidatud saksa mängufilmis "Engel & Joe" (režissöör Vanessa Jopp) ühes peaosas esines festivali külastanud saksa näitleja Robert Stadlober (1982). Näitleja Engeli rollist ja oma elukogemusest, mis aitas seda rolli kehastada

  6. Embryonic stem cell-like cells derived from adult human testis

    NARCIS (Netherlands)

    Mizrak, S. C.; Chikhovskaya, J. V.; Sadri-Ardekani, H.; van Daalen, S.; Korver, C. M.; Hovingh, S. E.; Roepers-Gajadien, H. L.; Raya, A.; Fluiter, K.; de Reijke, Th M.; de la Rosette, J. J. M. C. H.; Knegt, A. C.; Belmonte, J. C.; van der Veen, F.; de rooij, D. G.; Repping, S.; van Pelt, A. M. M.

    2010-01-01

    Given the significant drawbacks of using human embryonic stem (hES) cells for regenerative medicine, the search for alternative sources of multipotent cells is ongoing. Studies in mice have shown that multipotent ES-like cells can be derived from neonatal and adult testis. Here we report the

  7. Avatud ühiskond ja suletud ruum / Donald Tomberg

    Index Scriptorium Estoniae

    Tomberg, Donald, 1972-

    2009-01-01

    Prantsusmaa 2008. a. mängufilmist "Seinte vahel" ("Entre les murs"). Pooldokumentaalne lugu probleemsest klassikollektiivist Prantsusmaa ühes riigikoolis, stsenarist oma romaani alusel ning peaosa täitja Francois Begaudeau, režissöör Laurent Cantet

  8. Eliidi kesksusest / Fredo Arias-King ; tõlk. Riita-Ilona Märka

    Index Scriptorium Estoniae

    Arias-King, Fredo

    2003-01-01

    Eliidi- ja juhtimisteooria ei pruugi seletada, miks ühes riigis tekkis demokraatia ja teises mitte, kuid ta näitab põhjuseid, miks mõnes postkommunistlikus riigis toimus edukas üleminek demokraatiale või turumajandusele, teises see aga ebaõnnestus. Tabelid. Tõlgitud käsikirjast "The centrality of elites"

  9. Islamifilosoof : Euroopat ähvardab "kokkupõrge tsivilisatsiooni sees" / Ahto Lobjakas

    Index Scriptorium Estoniae

    Lobjakas, Ahto, 1970-

    2006-01-01

    Tariq Ramadani esinemisest Brüsselis islamiteemalisel konverentsil, mille korraldasid Euroopa Parlamendi rohelised. T. Ramadan on seisukohal, et nähes muslimistes esmalt islamiusulisi ja alles siis kodanikke, riskib Euroopa tsivilisatsioonisisese kokkupõrke võimalusega. Lisa: Oponent näeb islamis takistust

  10. A templated approach for multi-physics modeling of hybrid energy systems in Modelica

    Energy Technology Data Exchange (ETDEWEB)

    Greenwood, Michael Scott [ORNL; Cetiner, Sacit M. [ORNL; Harrison, Thomas J. [ORNL; Fugate, David [Oak Ridge National Laboratory (ORNL)

    2018-01-01

    A prototypical hybrid energy system (HES) couples a primary thermal power generator (i.e., nuclear power plant) with one or more additional subsystems beyond the traditional balance of plant electricity generation system. The definition and architecture of an HES can be adapted based on the needs and opportunities of a given local market. For example, locations in need of potable water may be best served by coupling a desalination plant to the HES. A location near an oil refinery may have a need for emission-free hydrogen production. The flexible, multidomain capabilities of Modelica are being used to investigate the dynamics (e.g., thermal hydraulics and electrical generation/consumption) of such a hybrid system. This paper examines the simulation infrastructure created to enable the coupling of multiphysics subsystem models for HES studies. A demonstration of a tightly coupled nuclear hybrid energy system implemented using the Modelica based infrastructure is presented for two representative cases. An appendix is also included providing a step-by-step procedure for using the template-based infrastructure.

  11. The efficacy of two bupivacaine hydrochloride injection products

    African Journals Online (AJOL)

    1996-06-06

    Jun 6, 1996 ... stances majority of o the survey t the time hamstrung g staff. itive hes to ... hydrochloride injection products was investigated in patients who ... upon the concentration, dose, route of application, and ... Practice guidelines.~.5 .... GUIdelines on Ihe Qualoty. safety and efficacy Of mra.cmal prOducts for human.

  12. São Paulo majad kõnelevad salakeeles / Filipp Kruusvall

    Index Scriptorium Estoniae

    Kruusvall, Filipp

    2015-01-01

    Artikkel tutvustab São Paulo tänavakunstnikke pixadoreseid, kes ronivad kõrghoonetele joonistama oma märguannet sotsiaalsest elususest. See illegaalne tegevus on ühtaegu hääl, sõnum kui ka karje ebaõigluse vastu ühes maailma ebaühtlasema varalise kihistumisega riigis Brasiilias

  13. Alternatives to the Fish Early Life-Stage Test: A Research Strategy for Discovering and Annotating Adverse Outcome Pathways During Fish Development

    Science.gov (United States)

    The OECD 210 fish early life]stage (FELS) test is the primary guideline test used to estimate chronic fish toxicity, as well as support ecological risk assessments and chemical management programs around the world. As a step toward developing alternatives to the FELS test, a HES...

  14. Riigijuhtide riskialdis õhulend / Oliver Rand

    Index Scriptorium Estoniae

    Rand, Oliver, 1971-

    2007-01-01

    President Toomas Hendrik Ilves, Riigikogu esimees Ene Ergma, kaitseminister Jaak Aaviksoo, kaitseväe juhataja Ants Laaneots, Riigikogu riigikaitsekomisjoni esimees Mati Raidma koos kaaskonnaga lendasid USA lennukikandjale USS Enterprise ühes lennukis. Riigi võtmeisikute üheskoos reisimisega kaasnevatest turvariskidest. Vt. samas: Mujal karmimad meetmed

  15. Effect of volume loading on the Frank-Starling relation during reductions in central blood volume in heat-stressed humans

    DEFF Research Database (Denmark)

    Bundgaard-Nielsen, Morten; Wilson, T E; Seifert, Thomas

    2010-01-01

    -body heating after intravascular volume expansion. Volume expansion was accomplished by administration of a combination of a synthetic colloid (HES 130/0.4, Voluven) and saline. Before LBNP, SV was not affected by heating (122 +/- 30 ml; mean +/- s.d.) compared to normothermia (110 +/- 20 ml; P = 0...

  16. Normovolemia defined according to cardiac stroke volume in healthy supine humans

    DEFF Research Database (Denmark)

    Bundgaard-Nielsen, Morten; Jørgensen, Christoffer C; Kehlet, Henrik

    2010-01-01

    , mean +/- SD), SV was measured by esophageal Doppler before and after fluid administration to evaluate whether SV increases in healthy, non-fasting, supine subjects. Two hundred millilitres of a synthetic colloid (hydroxyethyl starch, HES 130/0.4) was provided and repeated if a >or=10% increment in SV...

  17. Nicolas Cage otsib kadunud tütart naistesaarelt / Triin Tael

    Index Scriptorium Estoniae

    Tael, Triin

    2006-01-01

    Õudusfilm "Ohvripidu" : režissöör Neil LaBute : peaosas Nicholas Cage : Ameerika Ühendriigid - Saksamaa 2006. Film on uusversioon briti 1973.a. filmist, mille režissöör oli Robin Hardy ja ühes peaosadest Christopher Lee

  18. Moodsa reklaami surm / Maurice Saatchi ; tõlk. Erik Aru

    Index Scriptorium Estoniae

    Saatchi, Maurice

    2006-01-01

    Autori sõnul võistlevad turunduse uues ärimudelis firmad selle pärast, kellele kuulub avalikus teadvuses ühe sõna ilmne omandiõigus. Näiteks sõna "otsing" kuulub Google'ile, Sonyl oli sõna "innovatsioon". Ühesõnaline kapital on uute tehnoloogiate Uue Maailma kõige hindamatum vara

  19. LANGUAGE STYLE OF HABIBURRAHMAN EL-SHIRAZY IN THE DWILOGY OF AYAT-AYAT CINTA: A STYLISTIC STUDY

    Directory of Open Access Journals (Sweden)

    Aflahah Aflahah

    2017-05-01

    Full Text Available Novel is an artwork which closely relates to human life and is considered as the representation of human life journey. The language style in novel is the embodiment of language use by an author to express ideas, emotion, opinion, and to give a certain effect. The main problem will be discussed in this study is the language style found in the novels of AAC 1 and 2. This study is about individual language style, an author’s language style who have written best seller novels, namely Habiburrahman El-Shirazy (HES. The approach that is considered very appropriate in understanding the language use of HES in Dwilogy of Ayat-Ayat Cinta (DAAC is stylistic study. A stylistic study reveals how is the language style used by HES and what effects that are resulted. The approach of this research is a descriptive qualitative research. Descriptive method is used to describe the linguistic facts such as the language style based on lexical choice, sentence structure, and direct or indirect meaning. The use of language style based on lexical choice (diction in DAAC shows a typical language style of HES as well as shows his ability as a Da’i and man of letters. The analysis of language style based on sentence structure and direct/indirect meaning illustrates the descriptive style of HES. Whatever he describes, it must be very accurate. He gives very detail information of the object being spoken of either background of the story or characterization. Through language style, HES describes the emotions experienced by the characters properly so it makes the readers able to empathize. From the data had been obtained, it reveals that language style used by HES to describe characters or characterization (describe the physical condition, characters, and characteristics, describes its background, tells the plot, and convey the message. The results showed the lexical choice of scientific words and religious words are typical of HES’s language style in his DAAC, the

  20. Testing of Strategies for the Acceleration of the Cost Optimization

    Energy Technology Data Exchange (ETDEWEB)

    Ponciroli, Roberto [Argonne National Lab. (ANL), Argonne, IL (United States); Vilim, Richard B. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2017-08-31

    The general problem addressed in the Nuclear-Renewable Hybrid Energy System (N-R HES) project is finding the optimum economical dispatch (ED) and capacity planning solutions for the hybrid energy systems. In the present test-problem configuration, the N-R HES unit is composed of three electrical power-generating components, i.e. the Balance of Plant (BOP), the Secondary Energy Source (SES), and the Energy Storage (ES). In addition, there is an Industrial Process (IP), which is devoted to hydrogen generation. At this preliminary stage, the goal is to find the power outputs of each one of the N-R HES unit components (BOP, SES, ES) and the IP hydrogen production level that maximizes the unit profit by simultaneously satisfying individual component operational constraints. The optimization problem is meant to be solved in the Risk Analysis Virtual Environment (RAVEN) framework. The dynamic response of the N-R HES unit components is simulated by using dedicated object-oriented models written in the Modelica modeling language. Though this code coupling provides for very accurate predictions, the ensuing optimization problem is characterized by a very large number of solution variables. To ease the computational burden and to improve the path to a converged solution, a method to better estimate the initial guess for the optimization problem solution was developed. The proposed approach led to the definition of a suitable Monte Carlo-based optimization algorithm (called the preconditioner), which provides an initial guess for the optimal N-R HES power dispatch and the optimal installed capacity for each one of the unit components. The preconditioner samples a set of stochastic power scenarios for each one of the N-R HES unit components, and then for each of them the corresponding value of a suitably defined cost function is evaluated. After having simulated a sufficient number of power histories, the configuration which ensures the highest profit is selected as the optimal

  1. Using 'nudge' principles for order set design: a before and after evaluation of an electronic prescribing template in critical care.

    Science.gov (United States)

    Bourdeaux, Christopher P; Davies, Keith J; Thomas, Matthew J C; Bewley, Jeremy S; Gould, Timothy H

    2014-05-01

    Computerised order sets have the potential to reduce clinical variation and improve patient safety but the effect is variable. We sought to evaluate the impact of changes to the design of an order set on the delivery of chlorhexidine mouthwash and hydroxyethyl starch (HES) to patients in the intensive care unit. The study was conducted at University Hospitals Bristol NHS Foundation Trust, UK. Our intensive care unit uses a clinical information system (CIS). All drugs and fluids are prescribed with the CIS and drug and fluid charts are stored within a database. Chlorhexidine mouthwash was added as a default prescription to the prescribing template in January 2010. HES was removed from the prescribing template in April 2009. Both interventions were available to prescribe manually throughout the study period. We conducted a database review of all patients eligible for each intervention before and after changes to the configuration of choices within the prescribing system. 2231 ventilated patients were identified as appropriate for treatment with chlorhexidine, 591 before the intervention and 1640 after. 55.3% were prescribed chlorhexidine before the change and 90.4% after (p<0.001). 6199 patients were considered in the HES intervention, 2177 before the intervention and 4022 after. The mean volume of HES infused per patient fell from 630 mL to 20 mL after the change (p<0.001) and the percentage of patients receiving HES fell from 54.1% to 3.1% (p<0.001). These results were well sustained with time. The presentation of choices within an electronic prescribing system influenced the delivery of evidence-based interventions in a predictable way and the effect was well sustained. This approach has the potential to enhance the effectiveness of computerised order sets.

  2. F-18 FDG PET scan findings in patients with pulmonary involvement in the hypereosinophilic syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jae Hoon; Kim, Tae Hoon; Yun, Mi Jin [College of Medicine, Yonsei University, Seoul (Korea, Republic of)] (and others)

    2005-08-15

    Hypereosinophilic syndrome (HES) is an infiltrative disease of eosinophils affecting multiple organs including the lung. F-18 2-fluoro-2-deoxyglucose (F-18 FDG) may accumulate at sites of inflammation or infection, making interpretation of whole body PET scan difficult in patients with cancer. This study was to evaluate the PET findings of HES with lung involvement and to find out differential PET features between lung malignancy and HES with lung involvement. F-18 FDG PET and low dose chest CT scan was performed for screening of lung cancer. Eight patients who showed ground-glass attenuation (GGA) and consolidation on chest CT scan with peripheral blood eosinophilia were included in this study. The patients with history of parasite infection, allergy and collagen vascular disease were excluded. CT features and FDG PET findings were meticulously evaluated for the distribution of GGA and consolidation and nodules on CT scan and mean and maximal SUV of abnormalities depicted on F-18 FDG PET scan. In eight patients, follow-up chest CT scan and FDG PET scan were done one or two weeks after initial study. F-18 FDG PET scan identified metabolically active lesions in seven out of eight patients. Maximal SUV was ranged from 2.8 to 10.6 and mean SUV was ranged from 2.2 to 7.2. Remaining one patient had maximal SUV of 1.3. On follow-up FDG PET scan taken on from one to four weeks later showed decreased degree of initially noted FDG uptakes or migration of previously noted abnormal FDG uptakes. Lung involvement in the HES might be identified as abnormal uptake foci on FDG PET scan mimicking lung cancer. Follow-up FDG PET and CT scan for the identification of migration or resolution of abnormalities and decrement of SUV would be of help for the differentiation between lung cancer and HES with lung involvement.

  3. F-18 FDG PET scan findings in patients with pulmonary involvement in the hypereosinophilic syndrome

    International Nuclear Information System (INIS)

    Lee, Jae Hoon; Kim, Tae Hoon; Yun, Mi Jin

    2005-01-01

    Hypereosinophilic syndrome (HES) is an infiltrative disease of eosinophils affecting multiple organs including the lung. F-18 2-fluoro-2-deoxyglucose (F-18 FDG) may accumulate at sites of inflammation or infection, making interpretation of whole body PET scan difficult in patients with cancer. This study was to evaluate the PET findings of HES with lung involvement and to find out differential PET features between lung malignancy and HES with lung involvement. F-18 FDG PET and low dose chest CT scan was performed for screening of lung cancer. Eight patients who showed ground-glass attenuation (GGA) and consolidation on chest CT scan with peripheral blood eosinophilia were included in this study. The patients with history of parasite infection, allergy and collagen vascular disease were excluded. CT features and FDG PET findings were meticulously evaluated for the distribution of GGA and consolidation and nodules on CT scan and mean and maximal SUV of abnormalities depicted on F-18 FDG PET scan. In eight patients, follow-up chest CT scan and FDG PET scan were done one or two weeks after initial study. F-18 FDG PET scan identified metabolically active lesions in seven out of eight patients. Maximal SUV was ranged from 2.8 to 10.6 and mean SUV was ranged from 2.2 to 7.2. Remaining one patient had maximal SUV of 1.3. On follow-up FDG PET scan taken on from one to four weeks later showed decreased degree of initially noted FDG uptakes or migration of previously noted abnormal FDG uptakes. Lung involvement in the HES might be identified as abnormal uptake foci on FDG PET scan mimicking lung cancer. Follow-up FDG PET and CT scan for the identification of migration or resolution of abnormalities and decrement of SUV would be of help for the differentiation between lung cancer and HES with lung involvement

  4. Upfront immunohistochemistry improves specificity of Helicobacter pylori diagnosis. A French pathology laboratory point of view.

    Science.gov (United States)

    Ginestet, Florent; Guibourg, Briac; Doucet, Laurent; Théreaux, Jérémie; Robaszkiewicz, Michel; Marcorelles, Pascale; Uguen, Arnaud

    2017-10-01

    There is no consensus about the histopathologic methods to detect Helicobacter pylori in gastric biopsies to date. We aimed to question about the value of upfront anti-H. pylori immunohistochemistry in this field. We led a retrospective study about the rate of H. pylori-positive gastric biopsies before and after the implementation of upfront immunohistochemistry, the inter-rater and intermethods agreements in H. pylori identification about Hematoxylin-Eosin Saffron (HES), Giemsa, and immunohistochemistry stains and the histopathologic features associated with low amounts of H. pylori. First, the rate of H. pylori-positive gastric biopsies significantly diminished after the implementation of upfront immunohistochemistry (from 21.15% to 12.56%, Ppylori infection before the use of immunohistochemistry. Secondly, immunohistochemistry was the most reproducible and performing stain (kappa values >0.80), but HES and Giemsa stains also presented good-to-very good agreements. Finally, less than 1% of gastric biopsies with inconspicuous H. pylori infection showed no mucosal injury pointing out that any HES-detected mucosal injury could help to preselect the gastric biopsies requiring ancillary stains for the detection of H. pylori. Albeit being considered as a gold standard in the detection of H. pylori, the interest of using immunohistochemistry as an upfront stain on gastric biopsies is still debated. In our opinion, its use in second line in case of ambiguous HE/HES-Giemsa result is more appropriate. Further effort is needed to optimize the inexpensive but feasible HE/HES-based detection of H. pylori. © 2017 John Wiley & Sons Ltd.

  5. Buffer capacity of 4% succinylated gelatin does not provide any advantages over acidic 6% hydroxyethyl starch 130/0.4 for acid-base balance during experimental mixed acidaemia in a porcine model.

    Science.gov (United States)

    Esche, V; Russ, M; Melzer, S; Grossmann, B; Boemke, W; Unger, J K

    2008-11-01

    Four percent gelatine is an alkaline compound due to NH2 groups, whereas 6% hydroxyethyl starch 130/0.4 (HES130) has acidic features. We investigated whether these solutions lead to differences in acid-base balance in pigs during acidaemia and correction of pH. Anaesthetized pigs were randomized to HES130 or gelatine infusion (n = 5 per group). Animals received acid infusion (0.4 M solution of lactic acid and HCl diluted in normal saline) and low tidal volume ventilation (6-7 mL kg(-1), PaCO2 of 80-85 mmHg, pH 7.19-7.24). Measurements were made before and after induction of acidaemia, before and after correction of pH with haemofiltration (continuous venovenous haemofiltration) and tris-hydroxymethylaminomethane infusion. We measured parameters describing acid-base balance according to Stewart's approach, ketone body formation, oxygen delivery, haemodynamics, diuresis and urinary pH. Acid-base balance did not differ significantly between the groups. In HES130-treated pigs, the haemodilution-based drop of haemoglobin (1.4 +/- 1.0 g dL(-1), median +/- SD) was paralleled by an increase in the cardiac output (0.5 +/- 0.4 L min(-1). Lacking increases in cardiac output, gelatine-treated pigs demonstrated a reduction in oxygen delivery (149.4 +/- 106.0 mL min(-1)). Tris-hydroxymethylaminomethane volumes required for pH titration to desired values were significantly higher in the gelatine group (0.7 +/- 0.1 mL kg(-1) h(-1) vs. HES130: 0.5 +/- 0.2 mL kg(-1) h(-1)). The buffer capacity of gelatine did not lead to favourable differences in acid-base balance in comparison to HES130.

  6. Elders Health Empowerment Scale: Spanish adaptation and psychometric analysis.

    Science.gov (United States)

    Serrani Azcurra, Daniel Jorge Luis

    2014-01-01

    Empowerment refers to patient skills that allow them to become primary decision-makers in control of daily self-management of health problems. As important the concept as it is, particularly for elders with chronic diseases, few available instruments have been validated for use with Spanish speaking people. Translate and adapt the Health Empowerment Scale (HES) for a Spanish-speaking older adults sample and perform its psychometric validation. The HES was adapted based on the Diabetes Empowerment Scale-Short Form. Where "diabetes" was mentioned in the original tool, it was replaced with "health" terms to cover all kinds of conditions that could affect health empowerment. Statistical and Psychometric Analyses were conducted on 648 urban-dwelling seniors. The HES had an acceptable internal consistency with a Cronbach's α of 0.89. The convergent validity was supported by significant Pearson's Coefficient correlations between the HES total and item scores and the General Self Efficacy Scale (r= 0.77), Swedish Rheumatic Disease Empowerment Scale (r= 0.69) and Making Decisions Empowerment Scale (r= 0.70). Construct validity was evaluated using item analysis, half-split test and corrected item to total correlation coefficients; with good internal consistency (α> 0.8). The content validity was supported by Scale and Item Content Validity Index of 0.98 and 1.0, respectively. HES had acceptable face validity and reliability coefficients; which added to its ease administration and users' unbiased comprehension, could set it as a suitable tool in evaluating elder's outpatient empowerment-based medical education programs.

  7. Elders Health Empowerment Scale

    Science.gov (United States)

    2014-01-01

    Introduction: Empowerment refers to patient skills that allow them to become primary decision-makers in control of daily self-management of health problems. As important the concept as it is, particularly for elders with chronic diseases, few available instruments have been validated for use with Spanish speaking people. Objective: Translate and adapt the Health Empowerment Scale (HES) for a Spanish-speaking older adults sample and perform its psychometric validation. Methods: The HES was adapted based on the Diabetes Empowerment Scale-Short Form. Where "diabetes" was mentioned in the original tool, it was replaced with "health" terms to cover all kinds of conditions that could affect health empowerment. Statistical and Psychometric Analyses were conducted on 648 urban-dwelling seniors. Results: The HES had an acceptable internal consistency with a Cronbach's α of 0.89. The convergent validity was supported by significant Pearson's Coefficient correlations between the HES total and item scores and the General Self Efficacy Scale (r= 0.77), Swedish Rheumatic Disease Empowerment Scale (r= 0.69) and Making Decisions Empowerment Scale (r= 0.70). Construct validity was evaluated using item analysis, half-split test and corrected item to total correlation coefficients; with good internal consistency (α> 0.8). The content validity was supported by Scale and Item Content Validity Index of 0.98 and 1.0, respectively. Conclusions: HES had acceptable face validity and reliability coefficients; which added to its ease administration and users' unbiased comprehension, could set it as a suitable tool in evaluating elder's outpatient empowerment-based medical education programs. PMID:25767307

  8. Linkage of Maternity Hospital Episode Statistics data to birth registration and notification records for births in England 2005–2014: Quality assurance of linkage of routine data for singleton and multiple births

    Science.gov (United States)

    2018-01-01

    Objectives To quality assure a Trusted Third Party linked data set to prepare it for analysis. Setting Birth registration and notification records from the Office for National Statistics for all births in England 2005–2014 linked to Maternity Hospital Episode Statistics (HES) delivery records by NHS Digital using mothers’ identifiers. Participants All 6 676 912 births that occurred in England from 1 January 2005 to 31 December 2014. Primary and secondary outcome measures Every link between a registered birth and an HES delivery record for the study period was categorised as either the same baby or a different baby to the same mother, or as a wrong link, by comparing common baby data items and valid values in key fields with stepwise deterministic rules. Rates of preserved and discarded links were calculated and which features were more common in each group were assessed. Results Ninety-eight per cent of births originally linked to HES were left with one preserved link. The majority of discarded links were due to duplicate HES delivery records. Of the 4854 discarded links categorised as wrong links, clerical checks found 85% were false-positives links, 13% were quality assurance false negatives and 2% were undeterminable. Births linked using a less reliable stage of the linkage algorithm, births at home and in the London region, and with birth weight or gestational age values missing in HES were more likely to have all links discarded. Conclusions Linkage error, data quality issues, and false negatives in the quality assurance procedure were uncovered. The procedure could be improved by allowing for transposition in date fields, and more discrimination between missing and differing values. The availability of identifiers in the datasets supported clerical checking. Other research using Trusted Third Party linkage should not assume the linked dataset is error-free or optimised for their analysis, and allow sufficient resources for this. PMID:29500200

  9. Therapeutic plasma exchange in a single center: Ibni Sina experience.

    Science.gov (United States)

    Arslan, Onder; Arat, Mutlu; Tek, Ibrahim; Ayyildiz, Erol; Ilhan, Osman

    2004-06-01

    The number of therapeutic procedures is increasing steadily year by year with growing collaboration of departments other than Hematology. In the aim to demonstrate our single center activity we analyzed our data since four years. Between years 1998 and 2001, 658 therapeutic plasma exchange (TPE) procedures were performed on 158 patients. Median age and male/female ratio were 37 (range, 15-87) and 80/78, respectively. Main indications were myastenia gravis (n=55, 34%), TTP (n=13, 8.5%), post ABO mismatched allogeneic hematopoietic cell transplantation aregeneratoric anemia (n=6, 7.5%), progressive systemic sclerosis (n=10, 6.5%), multiple myeloma (n=10, 6.5%), Gullian Barre Syndrome (n=9, 5.9%), multiple sclerosis (n=7, 4.6%), Waldenström Macroglobulinemia (n=5, 3.4%), polymyositis (n=4, 2.7%), sepsis and disseminated intravascular coagulation (n=4, 2.7%). Departments who referred the majority of the patients for TPE were neurology (n=199), hematology (n=153), immunology (n=78), intensive care unit (n=78) and thorax surgery (n=51). The median TPE procedure per patient was 4 (range, 1-50). All the procedures were performed on continuous flow cell separators and median plasma volume processed per cycle was 2471 ml (range 436-5000). The replacement fluids used were 3% hydroxyethylstarch (HES) (24%), 5% albumin (35%), fresh frozen plasma (25%), and HES and albumin (16%). HES was tolerated well even as a sole replacement fluid with acceptable minor side effects. In three patients with progressing hypoalbuminemia HES was replaced or combined with 5% albumin. Close monitoring of serum albumin and fibrinogen levels after repeated procedures is mandatory. In our four years of TPE experience we have increased our collaboration with other departments. 3% HES+/-5% Albumin is a feasible, well tolerated and cost effective replacement fluid combination especially for short-term plasma exchange therapy.

  10. Natural history of severe eosinophilia with uncertain aetiology and proposals on a practical approach to its management.

    Science.gov (United States)

    Ang, A L; Wong, R X; Zhuang, Q Y; Linn, Y C

    2012-08-01

    Eosinophilia is commonly encountered during clinical practice. Some can be attributed to well-defined causes while others cannot. Optimal management of hypereosinophilia with unknown aetiology is uncertain as the natural history is not well described. We retrospectively studied patients with hypereosinophilia (>5 × 10(9)/L) and described the characteristics, natural history and treatment of those with eosinophilia of uncertain aetiology. There were 141 patients with hypereosinophilia: 87 with well-defined causes, 54 with uncertain aetiology. The latter was managed as hypereosinophilic syndrome (HES) (n = 5), idiopathic hypereosinophilia (IH) (n = 11), presumptive helminthic infection (n = 11) and reactive eosinophilia (n = 5), while 22 were insufficiently investigated and did not have definite working diagnoses. Their median age and peak eosinophil count were 64 (22 to 94) years and 10.0 (5.2-33.9) × 10(9)/L respectively. Forty-six per cent had symptoms attributable to eosinophilia, with the HES and insufficiently investigated groups having the highest (100%) and lowest (27%) percentages respectively. HES and IH patients were most extensively investigated. All 14 HES or IH patients who received steroids responded. All presumptive helminthic infection patients received mebendazole: nine responded, and two had unassessable responses. For the remaining patients, seven received steroids and all responded; one received mebendazole but defaulted; 19 were not treated: 11 resolved spontaneously. No non-HES patients developed eosinophilia-related organ dysfunction. No mortality was caused by hypereosinophilia. Patients with hypereosinophilia of uncertain aetiology can be empirically managed according to working diagnoses derived from history taking, examination and selective investigations. Most patients have benign short-term outcomes, but longer monitoring is required to assess long-term outcomes from untreated hypereosinophilia. © 2011 The Authors. Internal Medicine

  11. WATERSHED INFARCTION IN HYPEREOSINOPHILIC SYNDROME: A DIAGNOSTIC DILEMMA IN FIP1L1-PDGFR ALPHA-ASSOCIATED MYELOID NEOPLASM.

    Science.gov (United States)

    Marton, Imelda; Pósfai, Éva; Annus, János Kristóf; Borbényi, Zita; Nemes, Attila; Vecsei, László; Vörös, Erika

    2015-05-30

    The FIP1L1-PDGFR alpha-positive, hypereosinophilic syndrome (HES) is a new category of hematological entities. Various clinical symptoms may occur, with no specific characteristics in either the clinical picture or the neuroimaging findings, and this may give rise to a diagnostic dilemma. A report on a long follow-up period (10 years) in a case of HES that presented with neuropsychiatric symptoms appears to be unique. Besides the complexity of the diagnostic process, the successful treatment is discussed. The HES was diagnosed in a male patient at the age of 33 years, with involvement of the central nervous system and the myocardium. After the onset of the clinical signs, the MRI indicated bilateral cerebral and cerebellar cortico-subcortical lesions involving the watershed areas, mainly in the parieto-occipital regions. High-dose intravenous steroid (methylprednisolone 500 mg/day) alleviated the neurological symptoms within a few weeks, and the administration of imatinib (200 mg/day) resulted in an impressive regression of the hypereosinophilia and splenomegaly within 6 weeks. During the follow-up, the patient has continued to receive imatinib. The molecular remission has persisted, no new complaints have developed and the condition of the patient has remained stable. The timely recognition of the HES and identification of the disease subtype which led to the administration of imatinib may be the key to successful treatment. The long stable follow-up period gives rise to a new dilemma in the treatment of the HES in these special cases: for how long should a patient receive a tyrosine kinase inhibitor, and may the treatment be suspended?

  12. Comparison of Hb-200 and 6% hetastarch 450/0.7 during initial fluid resuscitation of 20 dogs with gastric dilatation-volvulus.

    Science.gov (United States)

    Haak, Carol E; Rudloff, Elke; Kirby, Rebecca

    2012-04-01

    To compare the use of polymerized stroma-free bovine hemoglobin (Hb-200) and 6% hetastarch 450/0.7 (HES 450/0.7) in 0.9% saline during fluid resuscitation of dogs with gastric dilatation-volvulus (GDV). Prospective, randomized clinical case series. Private specialty and referral clinic. Twenty client-owned dogs presenting with GDV. Dogs presenting with GDV and abnormal perfusion parameters first received rapid IV infusion of a buffered isotonic replacement crystalloid (15 mL/kg) and IV opioids. Patients were then randomized to receive either Hb-200 (N = 10) or HES 450/0.7 (N = 10). Balanced isotonic replacement crystalloids (10-20 mL/kg IV) were rapidly infused along with either Hb-200 or HES in 5 mL/kg IV aliquots to meet resuscitation end points. Resuscitation was defined as meeting at least 2 of 3 criteria: (1) capillary refill time 1-2 seconds, pink mucous membrane color, strong femoral pulse quality; (2) heart rate (HR) ≤ 150/min; or (3) indirect arterial systolic blood pressure (SBP) > 90 mm Hg. HR, SBP, packed cell volume, hemoglobin, glucose, venous pH, bicarbonate, base excess, anion gap, and colloid osmotic pressure were compared at hospital entry and within 30 minutes post-resuscitation. Compared to the HES group, the Hb-200 group required significantly less colloid (4.2 versus 18.4 mL/kg) and crystalloid (31.3 versus 48.1 mL/kg) to reach resuscitation end points (P = 0.001). Time to resuscitation was significantly shorter in the Hb-200 group (12.5 versus 52.5 min). Dogs with GDV receiving Hb-200 during initial resuscitation required smaller volumes of both crystalloid and colloid fluids and reached resuscitation end points faster than dogs receiving HES 450/0.7 (P = 0.02). © Veterinary Emergency and Critical Care Society 2012.

  13. Systematically profiling and annotating long intergenic non-coding RNAs in human embryonic stem cell.

    Science.gov (United States)

    Tang, Xing; Hou, Mei; Ding, Yang; Li, Zhaohui; Ren, Lichen; Gao, Ge

    2013-01-01

    While more and more long intergenic non-coding RNAs (lincRNAs) were identified to take important roles in both maintaining pluripotency and regulating differentiation, how these lincRNAs may define and drive cell fate decisions on a global scale are still mostly elusive. Systematical profiling and comprehensive annotation of embryonic stem cells lincRNAs may not only bring a clearer big picture of these novel regulators but also shed light on their functionalities. Based on multiple RNA-Seq datasets, we systematically identified 300 human embryonic stem cell lincRNAs (hES lincRNAs). Of which, one forth (78 out of 300) hES lincRNAs were further identified to be biasedly expressed in human ES cells. Functional analysis showed that they were preferentially involved in several early-development related biological processes. Comparative genomics analysis further suggested that around half of the identified hES lincRNAs were conserved in mouse. To facilitate further investigation of these hES lincRNAs, we constructed an online portal for biologists to access all their sequences and annotations interactively. In addition to navigation through a genome browse interface, users can also locate lincRNAs through an advanced query interface based on both keywords and expression profiles, and analyze results through multiple tools. By integrating multiple RNA-Seq datasets, we systematically characterized and annotated 300 hES lincRNAs. A full functional web portal is available freely at http://scbrowse.cbi.pku.edu.cn. As the first global profiling and annotating of human embryonic stem cell lincRNAs, this work aims to provide a valuable resource for both experimental biologists and bioinformaticians.

  14. Volume Replacement Therapy during Hip Arthroplasty using Hydroxyethyl Starch (130/0.4 Compared to Lactated Ringer Decreases Allogeneic Blood Transfusion and Postoperative Infection

    Directory of Open Access Journals (Sweden)

    Adilson Hamaji

    2013-01-01

    Full Text Available Background and objectives: Hydroxyethyl starch (HES 130/0.4 is considered an effective plasma expander when compared to crystalloids. There is controversy around its superiority regarding hemodynamic optimization and about possible detrimental effects on coagulation. The aim of this study was to compare the effects of HES 130/0.4 to lactated Ringer solution during hip arthroplasty in adult patients under spinal anesthesia regarding intraoperative bleeding, hemodynamic parameters, coagulation profile, transfusion requirements and clinical outcomes. Methods: In this randomized, controlled trial, 48 patients scheduled for hip arthroplasty with spinal anesthesia were randomized into two groups: 24 patients were allocated to receive a preload of 15 mL.kg-1 of HES 130/0.4 and 24 patients received a preload of 30 mL.kg-1 lactated Ringer solution before surgery. Hemodynamic measurements, hemoglobin concentrations, biochemical parameters and coagulation tests were evaluated in three periods during surgical procedure. Patients received medical follow-up during their hospital stay and up to postoperative 30 days. Primary outcome was the requirement of red blood cell transfusion between groups during hospital stay. Secondary outcome were hemodynamic parameters, length of hospital stay, mortality and occurrence of clinical postoperative complications. Results: Red blood cell transfusion was required in 17% of patients in the HES group and in 46% in the Ringer group (p = .029. Postoperative infections were more frequently observed in the Ringer group (17% compared to the HES group (0, p = .037. There were no significant differences between groups in mortality, hospital length of stay and clinical complications other than infection. Conclusions: During hip arthroplasty, patients treated with hypervolemic hemodilution with hydroxyethyl starch 130/0.4 required less transfusion and presented lower infection rate compared to patients who received lactated

  15. Peroral endoscopic myotomy can improve esophageal motility in patients with achalasia from a large sample self-control research (66 patients.

    Directory of Open Access Journals (Sweden)

    Shuangzhe Yao

    Full Text Available Peroral endoscopic myotomy (POEM as a new approach to achalasia attracts broad attention. The primary objective of this study was to evaluate the results with esophageal motility after POEM through the first large sample clinical research.We have a self-control research with all patients (205 in total who underwent POEM from 2010 to 2014 at our Digestive Endoscopic Center, 66 patients of which underwent high resolution manometry (HRM before and after POEM in our motility laboratory. Follow-ups last for 5.6 months on average. Outcome variables analyzed included upper esophageal sphincter pressure (UESP, upper esophageal sphincter residual pressure (UESRP, lower esophageal sphincter pressure (LESP, lower esophageal sphincter residual pressure (LESRP and esophageal body peristalsis. We have a statistical analysis to illustrate how POEM impacts on the change of esophageal motility.The symptoms related to dysphagia were relieved in 95% of patients in recent term after POEM. While HRM showed a statistically significant reduction of URSRP, LESP and LESRP (P0.05 did not occur for these two groups on LESP and LESRP reduction.POEM clearly relieved the symptoms related to dysphagia by lowering the pressure of upper esophageal sphincter (UES and lower esophageal sphincter (LES,and other endoscopic treatment before POEM did not affect the improvement of LES pressure. These results are concluded from our short-term follow-up study, while the long-term efficacy remains to be further illustrated.Chinese Clinical Trial Register ChiCTR-TRC-12002204.

  16. Ungaris eesti keelt õppivate üliõpilaste kommunikatiivne kompetents

    Directory of Open Access Journals (Sweden)

    Marju Ilves

    2006-05-01

    Full Text Available Kommunikatiivne kompetents on võime keelt tõhusalt ja olukorrale vastavalt kasutada, sisuliselt tähendab see suhtlemisoskust. Kommunikatiivse kompetentsi moodustavad leksikaalne, sotsiolingvistiline/sotsiokultuuriline ja pragmaatiline kompetents. Eesti keele õpetamine välismaal toimub peaasjalikult deskriptiivselt. Ungarlastele mõeldud eesti keele õpik pakub deskriptiivse materjali kõrval lugemispalu ja näitedialooge suhtlussituatsioonide kohta, kommunikatiivsed ülesanded õpikus puuduvad. Kommunikatiivse keeleõppemeetodi kasutamine keeletunnis valmistab keeleõppijat ette teatud tüüpilisteks suhtlussituatsioonideks, millega ta keelekeskkonda sattudes kohe kokku puutub. Koostasin ungarlastest eesti keelt õppinud/õppivatele üliõpilastele mõned lihtsamad suhtlussituatsiooni kirjeldused, milles oli antud suhtluseesmärk ning oli vaja leida keelelised vahendid selle lahendamiseks (nt Kuidas küsid tänaval kella?. Kontrollmaterjalina kasutasin Tartu Ülikooli eestlastest üliõpilaste ja Szombathely Kõrgkooli ungarlastest üliõpilaste vastuseid. Uurimusest selguvad mõned kultuurilised erinevused ja see, kuivõrd on Ungaris eesti keele õppija ette valmistatud autentseteks suhtlussituatsioonideks.

  17. Tekstisisesed seosed eesti keele kui teise keele lugemistestis: mõõtmisvahendi valiidsus

    Directory of Open Access Journals (Sweden)

    Tiina Kikerpill

    2013-10-01

    Full Text Available Teksti mõistmine on keerukas protsess olenemata sellest, kas tegu on emakeeles või võõrkeeles lugemisega, sest see kätkeb endas samaaegset sõnade tuvastamist, tekstiga väljendatud otsese tähenduse tuletamist ja lugeja taustteadmiste abil teksti tõlgendamist. Üheks teksti mõistmise aspektiks on oskus ära tunda tekstis sisalduvaid sisulis-loogilisi ja leksikaalseid seoseid. Seega peaks lugemisoskuse arendamisel ja mõõtmisel pöörama tähelepanu ka tekstisisestele seostele. Võõrkeele lugemistestides kasutatakse nende seoste mõistmise kontrollimiseks erinevaid ülesandetüüpe, kuid nende tõhusust on vähe uuritud. Artiklis analüüsitakse kolme ülesandetüüpi – tekstilõikude järjestamist ning lausete ja tekstilõikude sobitamist lünkadesse. Keskendutakse nende valiidsusele, otsides vastust küsimustele, mil määral sõltub ülesannete edukas sooritamine tekstisiseste seoste mõistmisest ning missugune on ülesandetüübi mõju tekstisiseste seoste kasutamisele ülesande soorituses.

  18. Nuclear-Renewable Energy Systems Secondary Product Market Analysis Study

    Energy Technology Data Exchange (ETDEWEB)

    Deason, Wesley Ray [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-06-01

    In order to properly create a program surrounding the development of any technological concept it is necessary to fully understand the market in which it is being developed. In the case of Integrated Nuclear-Renewable Hybrid Energy Systems (HES), there are two economic markets in which it must be able to participate in: the electricity market and the secondary product market associated with the specific system. The purpose of the present report is to characterize the secondary product market in the U.S. and to provide recommendations for further developing the HES program. While HESs have been discussed in depth in many other reports, it is helpful to discuss them briefly in the present work [REF]. The concept of the HES can be deduced to a system, featuring a combination of a nuclear power plant, a renewable energy source, and an industrial manufacturing plant . The system is designed in a fashion that allows it either to produce electricity or to manufacture a secondary product as needed. The primary benefit of this concept lies in its ability to maximize economic performance of the integrated system and to manufacture products in a carbon-free manner. A secondary benefit is the enhanced supply-side flexibility gained by allowing the HES to economically provide grid services. A key tenant to nuclear power plant economics in today’s electricity market is their ability to operate at a very high capacity factor. Unfortunately, in regions with a high penetration of renewable energy, the carbon free energy produced by nuclear power may not be needed at all times. This forces the nuclear power plant to find a user for its excess capacity. This may include paying the electric grid to find a user, releasing energy to the environment by ‘dumping steam’, or reducing power. If the plant is unable to economically or safely do any of these actions, the plant is at risk of being shutdown. In order to allow for nuclear power plants to continue to contribute carbon free

  19. Nuclear-Renewable Energy Systems Secondary Product Market Analysis Study

    International Nuclear Information System (INIS)

    Deason, Wesley Ray

    2015-01-01

    In order to properly create a program surrounding the development of any technological concept it is necessary to fully understand the market in which it is being developed. In the case of Integrated Nuclear-Renewable Hybrid Energy Systems (HES), there are two economic markets in which it must be able to participate in: the electricity market and the secondary product market associated with the specific system. The purpose of the present report is to characterize the secondary product market in the U.S. and to provide recommendations for further developing the HES program. While HESs have been discussed in depth in many other reports, it is helpful to discuss them briefly in the present work [REF]. The concept of the HES can be deduced to a system, featuring a combination of a nuclear power plant, a renewable energy source, and an industrial manufacturing plant . The system is designed in a fashion that allows it either to produce electricity or to manufacture a secondary product as needed. The primary benefit of this concept lies in its ability to maximize economic performance of the integrated system and to manufacture products in a carbon-free manner. A secondary benefit is the enhanced supply-side flexibility gained by allowing the HES to economically provide grid services. A key tenant to nuclear power plant economics in today's electricity market is their ability to operate at a very high capacity factor. Unfortunately, in regions with a high penetration of renewable energy, the carbon free energy produced by nuclear power may not be needed at all times. This forces the nuclear power plant to find a user for its excess capacity. This may include paying the electric grid to find a user, releasing energy to the environment by -dumping steam', or reducing power. If the plant is unable to economically or safely do any of these actions, the plant is at risk of being shutdown. In order to allow for nuclear power plants to continue to contribute carbon free

  20. Validating the use of Hospital Episode Statistics data and comparison of costing methodologies for economic evaluation: an end-of-life case study from the Cluster randomised triAl of PSA testing for Prostate cancer (CAP).

    Science.gov (United States)

    Thorn, Joanna C; Turner, Emma L; Hounsome, Luke; Walsh, Eleanor; Down, Liz; Verne, Julia; Donovan, Jenny L; Neal, David E; Hamdy, Freddie C; Martin, Richard M; Noble, Sian M

    2016-04-29

    To evaluate the accuracy of routine data for costing inpatient resource use in a large clinical trial and to investigate costing methodologies. Final-year inpatient cost profiles were derived using (1) data extracted from medical records mapped to the National Health Service (NHS) reference costs via service codes and (2) Hospital Episode Statistics (HES) data using NHS reference costs. Trust finance departments were consulted to obtain costs for comparison purposes. 7 UK secondary care centres. A subsample of 292 men identified as having died at least a year after being diagnosed with prostate cancer in Cluster randomised triAl of PSA testing for Prostate cancer (CAP), a long-running trial to evaluate the effectiveness and cost-effectiveness of prostate-specific antigen (PSA) testing. Both inpatient cost profiles showed a rise in costs in the months leading up to death, and were broadly similar. The difference in mean inpatient costs was £899, with HES data yielding ∼8% lower costs than medical record data (differences compatible with chance, p=0.3). Events were missing from both data sets. 11 men (3.8%) had events identified in HES that were all missing from medical record review, while 7 men (2.4%) had events identified in medical record review that were all missing from HES. The response from finance departments to requests for cost data was poor: only 3 of 7 departments returned adequate data sets within 6 months. Using HES routine data coupled with NHS reference costs resulted in mean annual inpatient costs that were very similar to those derived via medical record review; therefore, routinely available data can be used as the primary method of costing resource use in large clinical trials. Neither HES nor medical record review represent gold standards of data collection. Requesting cost data from finance departments is impractical for large clinical trials. ISRCTN92187251; Pre-results. Published by the BMJ Publishing Group Limited. For permission to use

  1. The Economic Potential of Three Nuclear-Renewable Hybrid Energy Systems Providing Thermal Energy to Industry

    Energy Technology Data Exchange (ETDEWEB)

    Ruth, Mark [National Renewable Energy Lab. (NREL), Golden, CO (United States); Cutler, Dylan [National Renewable Energy Lab. (NREL), Golden, CO (United States); Flores-Espino, Francisco [National Renewable Energy Lab. (NREL), Golden, CO (United States); Stark, Greg [National Renewable Energy Lab. (NREL), Golden, CO (United States); Jenkin, Thomas [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-12-01

    This report is one of a series of reports that Idaho National Laboratory and National Renewable Energy Laboratory are producing to investigate the technical and economic aspects of nuclear-renewable hybrid energy systems (N-R HESs). Previous reports provided results of an analysis of two N-R HES scenarios. This report builds that analysis with a Texas-synthetic gasoline scenario providing the basis in which the N-R HES sells heat directly to an industrial customer. Subsystems were included that convert electricity to heat, thus allowing the renewable energy subsystem to generate heat and benefit from that revenue stream. Nuclear and renewable energy sources are important to consider in the energy sector's evolution because both are considered to be clean and non-carbon-emitting energy sources.

  2. The Economic Potential of Nuclear-Renewable Hybrid Energy Systems Producing Hydrogen

    Energy Technology Data Exchange (ETDEWEB)

    Ruth, Mark [National Renewable Energy Lab. (NREL), Golden, CO (United States); Cutler, Dylan [National Renewable Energy Lab. (NREL), Golden, CO (United States); Flores-Espino, Francisco [National Renewable Energy Lab. (NREL), Golden, CO (United States); Stark, Greg [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2017-04-01

    This report is one in a series of reports that Idaho National Laboratory and the Joint Institute for Strategic Energy Analysis are publishing that address the technical and economic aspects of nuclear-renewable hybrid energy systems (N-R HESs). This report discusses an analysis of the economic potential of a tightly coupled N-R HES that produces electricity and hydrogen. Both low and high temperature electrolysis options are considered in the analysis. Low-temperature electrolysis requires only electricity to convert water to hydrogen. High temperature electrolysis requires less electricity because it uses both electricity and heat to provide the energy necessary to electrolyze water. The study finds that, to be profitable, the examined high-temperature electrosis and low-temperature electrosis N-R HES configurations that produce hydrogen require higher electricity prices, more electricity price volatility, higher natural gas prices, or higher capacity payments than the reference case values of these parameters considered in this analysis.

  3. A Non-Destructive Culturing and Cell Sorting Method for Cardiomyocytes and Neurons Using a Double Alginate Layer

    Science.gov (United States)

    Terazono, Hideyuki; Kim, Hyonchol; Hayashi, Masahito; Hattori, Akihiro; Nomura, Fumimasa; Kaneko, Tomoyuki; Yasuda, Kenji

    2012-01-01

    A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture. PMID:22870332

  4. U.S. Hydropower Resource Assessment - California

    Energy Technology Data Exchange (ETDEWEB)

    A. M. Conner; B. N. Rinehart; J. E. Francfort

    1998-10-01

    The U.S. Department of Energy is developing an estimate of the underdeveloped hydropower potential in the United States. For this purpose, the Idaho National Engineering and Environmental Laboratory developed a computer model called Hydropower Evaluation Software (HES). HES measures the undeveloped hydropower resources available in the United States, using uniform criteria for measurement. The software was developed and tested using hydropower information and data provided by the Southwestern Power Administration. It is a menu-driven program that allows the personal computer user to assign environmental attributes to potential hydropower sites, calculate development suitability factors for each site based on the environmental attributes present, and generate reports based on these suitability factors. This report describes the resource assessment results for the State of California.

  5. Comparability of a short food frequency questionnaire to assess diet quality: the DISCOVER study.

    Science.gov (United States)

    Dehghan, Mahshid; Ge, Yipeng; El Sheikh, Wala; Bawor, Monica; Rangarajan, Sumathy; Dennis, Brittany; Vair, Judith; Sholer, Heather; Hutchinson, Nichole; Iordan, Elizabeth; Mackie, Pam; Samaan, Zainab

    2017-09-01

    This study aims to assess comparability of a short food frequency questionnaire (SFFQ) used in the Determinants of Suicide: Conventional and Emergent Risk Study (DISCOVER Study) with a validated comprehensive FFQ (CFFQ). A total of 127 individuals completed SFFQ and CFFQ. Healthy eating was measured using Healthy Eating Score (HES). Estimated food intake and healthy eating assessed by SFFQ was compared with the CFFQ. For most food groups and HES, the highest Spearman's rank correlation coefficients between the two FFQs were r > .60. For macro-nutrients, the correlations exceeded 0.4. Cross-classification of quantile analysis showed that participants were classified between 46% and 81% into the exact same quantiles, while 10% or less were misclassified into opposite quantiles. The Bland-Altman plots showed an acceptable level of agreement between the two dietary measurement methods. The SFFQ can be used for Canadian with psychiatric disorders to rank them based on their dietary intake.

  6. U.S. hydropower resource assessment for Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Conner, A.M.; Francfort, J.E.

    1997-10-01

    The US Department of Energy is developing an estimate of the undeveloped hydropower potential in the US. The Hydropower Evaluation Software (HES) is a computer model that was developed by the Idaho National Engineering Laboratory for this purpose. HES measures the undeveloped hydropower resources available in the US, using uniform criteria for measurement. The software was developed and tested using hydropower information and data provided by the Southwestern Power Administration. It is a menu-driven program that allows the personal computer user to assign environmental attributes to potential hydropower sites, calculate development suitability factors for each site based on the environmental attributes present, and generate reports based on these suitability factors. This report describes the resource assessment results for the State of Nevada.

  7. A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.

    Directory of Open Access Journals (Sweden)

    Hideyuki Terazono

    Full Text Available A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.

  8. U.S. hydropower resource assessment for Idaho

    Energy Technology Data Exchange (ETDEWEB)

    Conner, A.M.; Francfort, J.E.

    1998-08-01

    The US Department of Energy is developing an estimate of the undeveloped hydropower potential in the US. The Hydropower Evaluation Software (HES) is a computer model that was developed by the Idaho National Engineering and Environmental Laboratory for this purpose. HES measures the undeveloped hydropower resources available in the US, using uniform criteria for measurement. The software was developed and tested using hydropower information and data provided by the Southwestern Power Administration. It is a menu-driven program that allows the personal computer user to assign environmental attributes to potential hydropower sites, calculate development suitability factors for each site based on the environmental attributes present, and generate reports based on these suitability factors. This report describes the resource assessment results for the State of Idaho.

  9. The Economic Potential of Three Nuclear-Renewable Hybrid Energy Systems Providing Thermal Energy to Industry

    International Nuclear Information System (INIS)

    Ruth, Mark; Cutler, Dylan; Flores-Espino, Francisco; Stark, Greg; Jenkin, Thomas

    2016-01-01

    This report is one of a series of reports that Idaho National Laboratory and National Renewable Energy Laboratory are producing to investigate the technical and economic aspects of nuclear-renewable hybrid energy systems (N-R HESs). Previous reports provided results of an analysis of two N-R HES scenarios. This report builds that analysis with a Texas-synthetic gasoline scenario providing the basis in which the N-R HES sells heat directly to an industrial customer. Subsystems were included that convert electricity to heat, thus allowing the renewable energy subsystem to generate heat and benefit from that revenue stream. Nuclear and renewable energy sources are important to consider in the energy sector's evolution because both are considered to be clean and non-carbon-emitting energy sources.

  10. Variabilidad en la utilización de los servicios de urgencias hospitalarios del Sistema Nacional de Salud Variability in Spanish National Health System hospital emergency services utilization

    Directory of Open Access Journals (Sweden)

    Salvador Peiró

    2010-01-01

    Full Text Available Objetivo: Los objetivos de este estudio fueron estimar las tasas de frecuentación a los servicios de urgencias hospitalarios (SUH del Sistema Nacional de Salud (SNS por áreas de salud, el porcentaje de ingresos, las razones estandarizadas de utilización de urgencias y analizar la relación con los recursos hospitalarios. Métodos: Estudio ecológico combinando información de diversas fuentes (Encuesta de Establecimientos Sanitarios con Régimen de Internado 2006 y Conjunto Mínimo de Datos Básicos 2006 para estimar la frecuentación a los SUH y el porcentaje de ingresos asociado en 164 áreas de salud de 14 comunidades autónomas (CC.AA.. Resultados: Los 35,3 millones de habitantes de las 164 áreas de salud incluidas realizaron 16,2 millones de visitas (45,7 por 100 habitantes a los SUH del SNS, y de ellos ingresaron algo más de 2 millones (12,6%. Excluyendo el 5% de áreas extremas por cada cola, la frecuentación osciló entre 31,6 y 78,7 urgencias/100 habitantes y el porcentaje de ingresos entre el 7,6% y el 27,9%. Estas diferencias apenas se atenuaron al estandarizar. El factor CC.AA. explicó un 29% de la varianza en frecuentación y un 82% en ingresos. La frecuentación no se asoció al volumen poblacional de camas o médicos de plantilla, aunque sí al de médicos de urgencias, hospitales de menor tamaño y no docentes. Conclusiones: Las áreas de salud del SNS mantienen una gran variabilidad en frecuentación a urgencias e ingresos por urgencias, que parece asociarse a un uso diferencial por problemas de menor entidad.Objective: The aims of this study were to estimate the rate of hospital emergency services (HES visits per health area, the associated percentage of admissions and the standardized HES utilization ratio, and to analyze their relationship with hospital resources. Methods: We performed an ecological study that combined information from distinct sources (Survey of Health Care Hospitalization Establishments 2006 and

  11. US hydropower resource assessment for Texas

    Energy Technology Data Exchange (ETDEWEB)

    Francfort, J.E.

    1993-12-01

    The Department of Energy is developing an estimate of the hydropower development potential in this country. The Hydropower Evaluation Software (HES) is a computer model that was developed by the Idaho National Engineering Laboratory for this purpose. The HES measures the potential hydropower resources available in the United States, using uniform criteria for measurement. The software was developed and tested using hydropower information and data provided by the Southwestern Power Administration. It is a dBASE menu-driven software application that allows the personal computer user to assign environmental attributes to potential hydropower sites, calculate development suitability factors for each site based on the environmental attributes present, and generate reports based on these suitability factors. This report details the resource assessment results for the state of Texas.

  12. US hydropower resource assessment for Montana

    Energy Technology Data Exchange (ETDEWEB)

    Francfort, J.E.

    1993-12-01

    The Department of Energy is developing an estimate of the hydropower development potential in this country. The Hydropower Evaluation Software (HES) is a computer model that was developed by the Idaho National Engineering Laboratory for this purpose. The HES measures the potential hydropower resources available in the United States, using uniform criteria for measurement. The software was developed and tested using hydropower information and data provided by the Southwestern Power Administration. It is a dBASE menu-driven software application that allows the personal computer user to assign environmental attributes to potential hydropower sites, calculate development suitability factors for each site based on the environmental attributes present, and generate reports based on these suitability factors. This report details the resource assessment results for the state of Montana.

  13. US hydropower resource assessment for Kansas

    Energy Technology Data Exchange (ETDEWEB)

    Francfort, J.E.

    1993-12-01

    The Department of Energy is developing an estimate of the hydropower development potential in this country. The Hydropower Evaluation Software (HES) is a computer model that was developed by the Idaho National Engineering Laboratory for this purpose. The HES measures the potential hydropower resources available in the United States, using uniform criteria for measurement. The software was developed and tested using hydropower information and data provided by the Southwestern Power Administration. It is a dBASE menu-driven software application that allows the personal computer user to assign environmental attributes to potential hydropower sites, calculate development suitability factors for each site based on the environmental attributes present, and generate reports based on these suitability factors. This report details the resource assessment results for the state of Kansas.

  14. Thromboelastography in patients with severe sepsis

    DEFF Research Database (Denmark)

    Haase, Nicolai; Ostrowski, Sisse Rye; Wetterslev, Jørn

    2015-01-01

    PURPOSE: To investigate the association between consecutively measured thromboelastographic (TEG) tracings and outcome in patients with severe sepsis. METHODS: Multicentre prospective observational study in a subgroup of the Scandinavian Starch for Severe Sepsis/Septic Shock (6S) Trial (NCT00962156......) comparing hydroxyethyl starch (HES) 130/0.42 vs. Ringer's acetate for fluid resuscitation in severe sepsis. TEG (standard and functional fibrinogen) was measured consecutively for 5 days, and clinical data including bleeding and death was retrieved from the trial database. Statistical analyses included Cox...... bleeding [HR 2.43 (1.16-5.07)] and possibly explained the excess bleeding with HES in the 6S trial. CONCLUSIONS: In our cohort of patients with severe sepsis, progressive hypocoagulability defined by TEG variables was associated with increased risk of death and increased risk of bleeding....

  15. Sepsis - tunnistaminen ja hoito ensi- ja akuuttihoidossa

    OpenAIRE

    Ansio, Susa; Saarenketo, Tiia

    2016-01-01

    Sepsiksellä tarkoitetaan tilannetta, jolloin ihmisen verenkierrossa on bakteereita ja tämä aiheuttaa vakavia oireita. Lähes kaikki sepsiksen oireet johtuvat elimistön puolustusreaktiosta bakteeria vastaan. Sepsiksen hoito on monivaiheista ja kallista, ja siihen kuolleisuus on suurta. Ensihoidossa olisi tärkeää tunnistaa mahdollinen sepsis ja aloittaa tarvittava hoito mahdollisimman varhaisessa vaiheessa. Opinnäytetyön tarkoituksena on kuvata sepsiksen tunnistamista ensihoidossa, myöhäisem...

  16. Metoodika lapse emotsionaalse väärkohtlemise hindamiseks institutsionaalses keskkonnas / Triin Edovald

    Index Scriptorium Estoniae

    Edovald, Triin

    2001-01-01

    Uurimusest, mille eesmärgiks oli lapse emotsionaalse väärkohtlemise hindamise metoodika kohandamine lastekodu tingimustele. Uurimus viidi läbi ühes Tartu lastekodus, intervjueerides kuut kasvatajat ja vaadeldes kuue lastekodulapse ja tema usalduskasvataja vahelist suhtlemist ja tegevust eesmärgiga sisse viia võimalikud täpsustused ja muutused emotsionaalse väärkohtlemise hindamise metoodikasse kohandades seda lastekodu tingimustele

  17. [Burnout syndrome in pre-hospital and hospital emergency. Cognitive study in two cohorts of nurses].

    Science.gov (United States)

    Cicchitti, Chiara; Cannizzaro, Giorgia; Rosi, Fabrizio; Maccaroni, Roberto; Menditto, Vincenzo G

    2014-01-01

    Burnout syndrome (BOS) associated with stress has been documented in health care professionals in many specialties. The emergency department and the pre-hospital healthcare services are highly stressful environments. Little is known about the BOS in critical care nursing staff. The objective of the study is to compare the incidence of BOS and its three domains, namely, emotional exhaustion, depersonalization and reduced professional accomplishment, in two cohorts of critical care nurses: a pre-hospital and a hospital emergency service. A survey using a questionnaire (the Maslach Burnout Inventory-General Survey, MBI-GS), among nurses of two Italian emergency services has been performed: a hospital emergency service (HES, Emergency Department or "Pronto Soccorso") and a pre-hospital emergency service (PHES, territorial healthcare service or "Centrale Operativa 118"). All 60 nurses surveyed (82% female) filled the questionnaires. BOS-related symptoms have been identified in at least 50% of the nurses in the HES: 50% suffered a medium-high emotional exhaustion, 75% had a medium-high depersonalization and 92.5% had a medium-high reduced professional accomplishment. Among the PEHS nurses, BOS-related symptoms have been identified in at least 60% of the respondents: 60% had a medium-high emotional exhaustion, 70% had a medium-high depersonalization and 95% had a medium-high reduced professional accomplishment. Moreover, the likelihood that a nurse has a severe BOS, that is at least one degree of high burnout or ≥2 degrees of medium burnout, is significantly higher in the group of the PHES than in the HES (90% vs 60%, p nursing staff had a severe BOS. The incidence of BOS appeared to be similar among PHES and HES nurses with a higher trend for the former. Further interventional studies are needed to investigate the determinants of BOS among critical care nurses and the potentially preventive strategies.

  18. The lunar tide in sporadic E

    Directory of Open Access Journals (Sweden)

    R. J. Stening

    1999-10-01

    Full Text Available It seems that the wind shear theory is accepted for the explanation of sporadic E at mid and low latitudes. Some examples from Arecibo are displayed to show this. The effect of lunar tides should then modify the wind-shear theory in a manner that yields the observed features of the lunar tide in the critical frequency foEs and the height h'Es of the sporadic E. This is shown to imply that the phase of the lunar tide in h'Es should be the same as the phase of the lunar tide in the eastward wind and that the phase of the lunar tide in foEs is three hours later. Hourly values of foEs, f bEs (the blanketing critical frequency and h'Es from several observatories are analysed for the lunar semidiurnal tide. It is found that the phase of the tide in foEs is often about 3 hours later than for h'Es in agreement with the theory. Seasonal variations in the tide are also examined with the statistically most significant results (largest amplitudes usually occurring in summer. After reviewing the many difficulties associated with determining the lunar tide in Es, both experimentally and theoretically, the analysed phase results are compared with what might be expected from Hagan's global scale wave model. Agreement is only fair (a success rate of 69% among the cases examined but probably as good as might be expected.Key words. Ionosphere (ionosphere – atmosphere interactions – ionospheric irregularities, Meteorology and atmosphere dynamics (waves and tides

  19. Ajaloo õppetunnid : õnn ja jumalik ettehooldus Johannes Schefferuse teoses "Memorabilium Sueticae gentis exemplorum liber singularis" (1671) / Kristiina Savin

    Index Scriptorium Estoniae

    Savin, Kristiina, 1973-

    2008-01-01

    Uppsala ülikooli poliitika ja kõnekunsti professori Johannes Schefferuse (1621-1679) ladina-keelsest teosest "Memorabilium Sueticae gentis exemplorum liber singularis" (Rootsi rahva mälestusväärsete näidete raamat ühes osas). Teoses esitatud uskumatutest lugudest. "Memorabilia" võtmemõisteks on voorus. Johannes Schefferuse isikust. Jumalakartlikkusest., õnnest ja edust. Ajaloost kui elu õpetajast

  20. Lineage specific expression of Polycomb Group Proteins in human embryonic stem cells in vitro.

    Science.gov (United States)

    Pethe, Prasad; Pursani, Varsha; Bhartiya, Deepa

    2015-05-01

    Human embryonic (hES) stem cells are an excellent model to study lineage specification and differentiation into various cell types. Differentiation necessitates repression of specific genes not required for a particular lineage. Polycomb Group (PcG) proteins are key histone modifiers, whose primary function is gene repression. PcG proteins form complexes called Polycomb Repressive Complexes (PRCs), which catalyze histone modifications such as H2AK119ub1, H3K27me3, and H3K9me3. PcG proteins play a crucial role during differentiation of stem cells. The expression of PcG transcripts during differentiation of hES cells into endoderm, mesoderm, and ectoderm lineage is yet to be shown. In-house derived hES cell line KIND1 was differentiated into endoderm, mesoderm, and ectoderm lineages; followed by characterization using RT-PCR for HNF4A, CDX2, MEF2C, TBX5, SOX1, and MAP2. qRT-PCR and western blotting was performed to compare expression of PcG transcripts and proteins across all the three lineages. We observed that cells differentiated into endoderm showed upregulation of RING1B, BMI1, EZH2, and EED transcripts. Mesoderm differentiation was characterized by significant downregulation of all PcG transcripts during later stages. BMI1 and RING1B were upregulated while EZH2, SUZ12, and EED remained low during ectoderm differentiation. Western blotting also showed distinct expression of BMI1 and EZH2 during differentiation into three germ layers. Our study shows that hES cells differentiating into endoderm, mesoderm, and ectoderm lineages show distinct PcG expression profile at transcript and protein level. © 2015 International Federation for Cell Biology.

  1. A SEARCH FOR UNRECOGNIZED CARBON-ENHANCED METAL-POOR STARS IN THE GALAXY

    International Nuclear Information System (INIS)

    Placco, Vinicius M.; Rossi, Silvia; Kennedy, Catherine R.; Beers, Timothy C.; Lee, Young Sun; Christlieb, Norbert; Sivarani, Thirupathi; Reimers, Dieter; Wisotzki, Lutz

    2010-01-01

    We have developed a new procedure to search for carbon-enhanced metal-poor (CEMP) stars from the Hamburg/ESO (HES) prism-survey plates. This method employs an extended line index for the CH G band, which we demonstrate to have superior performance when compared to the narrower G-band index formerly employed to estimate G-band strengths for these spectra. Although CEMP stars have been found previously among candidate metal-poor stars selected from the HES, the selection on metallicity undersamples the population of intermediate-metallicity CEMP stars (-2.5 ≤ [Fe/H] ≤ -1.0); such stars are of importance for constraining the onset of the s-process in metal-deficient asymptotic giant branch stars (thought to be associated with the origin of carbon for roughly 80% of CEMP stars). The new candidates also include substantial numbers of warmer carbon-enhanced stars, which were missed in previous HES searches for carbon stars due to selection criteria that emphasized cooler stars. A first subsample, biased toward brighter stars (B< 15.5), has been extracted from the scanned HES plates. After visual inspection (to eliminate spectra compromised by plate defects, overlapping spectra, etc., and to carry out rough spectral classifications), a list of 669 previously unidentified candidate CEMP stars was compiled. Follow-up spectroscopy for a pilot sample of 132 candidates was obtained with the Goodman spectrograph on the SOAR 4.1 m telescope. Our results show that most of the observed stars lie in the targeted metallicity range, and possess prominent carbon absorption features at 4300 A. The success rate for the identification of new CEMP stars is 43% (13 out of 30) for [Fe/H] < -2.0. For stars with [Fe/H] < -2.5, the ratio increases to 80% (four out of five objects), including one star with [Fe/H] < -3.0.

  2. The mobilization of hazardous elements after a tropical storm event in a polluted estuary

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Iruretagoiena, Azibar, E-mail: azibar.rodriguez@ehu.es [Department of Analytical Chemistry, University of the Basque Country, P.O. Box 664, 48080 Bilbao, Basque Country (Spain); Fdez Ortiz de Vallejuelo, Silvia; Diego, Alberto de [Department of Analytical Chemistry, University of the Basque Country, P.O. Box 664, 48080 Bilbao, Basque Country (Spain); Leão, Felipe B. de; Medeiros, Diego de; Oliveira, Marcos L.S.; Tafarel, Silvio R. [Environmental Science and Nanotechnology Department, Institute of Environmental Research and Human Development — IPADH, Capivari de Baixo, Santa Catarina (Brazil); Arana, Gorka; Madariaga, Juan Manuel [Department of Analytical Chemistry, University of the Basque Country, P.O. Box 664, 48080 Bilbao, Basque Country (Spain); Silva, Luis F.O., E-mail: felipeqma@hotmail.com [Environmental Science and Nanotechnology Department, Institute of Environmental Research and Human Development — IPADH, Capivari de Baixo, Santa Catarina (Brazil)

    2016-09-15

    The Tubarão River (Santa Catarina, Brazil) is affected by hazardous elements (HEs) pollution from abandoned coal mines, agricultural activities, urban discharges, industrial and leisure zones, etc. In order to study the distribution and sources of HEs contamination in a polluted estuary after a tropical storm, waters and surface sediments were collected from 15 sampling sites along the Tubarão River. The concentration of 24 elements (Ag, Al, As, Ba, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Mo, Ni, Pb, Sb, Se, Sn, Sr, Ti, Tl, V, W, and Zn) were measured by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and the mineralogical composition of the sediments by Raman spectroscopy and X-ray diffraction (XRD). The metal concentrations in water and sediment showed wide spatial variation due to the variability in water discharges and anthropogenic inputs after a storm. In general, higher metal concentration in water and lower in sediments were found upstream (closer to coal mining). Downstream sampling sites and the Oratorio River sampling site (one of the eight tributaries of the estuary) showed the highest values in sediment samples. Normalized and Weighed Average Concentrations (NWAC) were calculated, which allow us to identify, in a very simple way, the sampling sites of higher concern (hotspots of contamination) in the studied area. NWAC suggested that the strong rainfall events could affect to the metal distribution in sediments. The results of this study were compared with a previous study in the same area during dry season by Principal Component Analysis (PCA), showing changes in environmental pollution of the sediment after a strong storm event. - Highlights: • The highest water HEs concentrations were identified in the sampling sites closest to the coal mines. • The presence of gypsum downstream is due to the remobilisation of upstream sediments by storm flow. • The HEs composition of the Tubarao River sediments changes due to strong rainfall events.

  3. Valsartan ameliorates podocyte loss in diabetic mice through the Notch pathway.

    Science.gov (United States)

    Gao, Feng; Yao, Min; Cao, Yanping; Liu, Shuxia; Liu, Qingjuan; Duan, Huijun

    2016-05-01

    The Notch pathway is known to be linked to diabetic nephropathy (DN); however, its underlying mechanism was poorly understood. In the present study, we examined the effect of Valsartan, an angiotensin II type 1 receptor antagonist, on the Notch pathway and podocyte loss in DN. Diabetes was induced in mice by an intraperitoneal injection of streptozotocin and and this was followed by treatment with Valsartan. Levels of blood glucose, kidney weight and body weight, as well as proteinuria were measured. Samples of the kidneys were also histologically examined. The relative levels of Jagged1, Notch1, Notch intracellular domain 1 (NICD1), Hes family BHLH transcription factor 1 (Hes1) and Hes-related family BHLH transcription factor with YRPW motif 1 expression (Hey1) in the glomeruli were determined by immunohistochemical analysis, western blot analysis and RT-qPCR. The B-Cell CLL/Lymphoma 2 (Bcl-2) and p53 pathways were examined by western blot analysis. Apoptosis and detachment of podocytes from the glomerular basement membrane were examined using a TUNEL assay, flow cytometric analysis and ELISA. The number of podocytes was quantified by measuring Wilms tumor-1 (WT-1) staining. We noted that the expression of Jagged1, Notch1, NICD1, Hes1 and Hey1 was increased in a time-dependent manner in the glomeruli of mice with streptozotocin (STZ)-induced diabetes. Moreover, in diabetic mice, Valsartan significantly reduced kidney weight and proteinuria, and mitigated the pathogenic processes in the kidneys. Valsartan also inhibited the activation of Notch, Bcl-2 and p53 pathways and ameliorated podocyte loss in the glomeruli of mice with STZ-induced diabetes. Taken together, these findings indicated that Valsartan exerted a beneficial effect on reducing podocyte loss, which is associated with inhibition of Notch pathway activation in the glomeruli of diabetic mice.

  4. Utilization of Local Ingredients for the Production of High-Early-Strength Engineered Cementitious Composites

    Directory of Open Access Journals (Sweden)

    Hanwen Deng

    2018-01-01

    Full Text Available The rapid repair and retrofitting of existing transportation infrastructure requires dimensional stability and ductile repair material that can obtain sufficiently high strength in a few hours to accommodate the large loading and deformation at an early age. Engineering cementitious composites (ECCs is a class representative of the new generation of high-performance fiber-reinforced cement-based composites (HPFRCC with medium fiber content. The unique properties of tremendous ductility and tight multiple crack behavior indicate that ECC can be used as an effective retrofit material. The wide application of this material in China will require the use of all local ingredients. In this study, based on Chinese domestic ingredients, including matrix materials and all fibers, high-early-strength ECC (HES-ECC was designed under the guidance of strain-hardening criterion of ECC. The matrix properties and fiber/matrix interfacial micromechanics properties were obtained from three-point-bending test and single-fiber pullout test. The mechanical properties of HES-ECC were achieved by direct tensile test. The experimental results show that HES-ECC was successfully developed by using all Chinese materials. When using the domestic PVA fiber at 2%, the strength requirement can be achieved but only a low ductility. When using the domestic PE fiber at 0.8%, the strength and deformation requirement both can be obtained. The HES-ECC developed in this study exhibited compressive strength of more than 25 MPa within 6 hours, and an ultimate tensile strength of 5-6 MPa and tensile strain capacity of 3-4% after 60 days. Moreover, the cost of using domestic fiber can be largely reduced compared with using imported fiber, up to 70%; it is beneficial to the promotion of these high-early-strength ECCs in the Chinese market.

  5. ORION - A Global Approach to Waste Management.

    Science.gov (United States)

    Heinzelmann, Elsbeth

    2015-01-01

    In the ORION project supported by the European Commission, 20 partners work together to manage organic waste from agro-food industries. The goal is to develop a small, automatic and user-friendly digestion machine to facilitate the domestic on-site treatment of a wide range of organic waste from around 100 and up to 5000 tonnes per year at low cost and with limited maintenance. Simon Crelier at the HES-SO Valais/Wallis is part of the network.

  6. Non invasive characterization of differentiation processes in human stem cells

    OpenAIRE

    Hildebrandt, Cornelia

    2011-01-01

    Gegenstand dieser Arbeit war die vergleichende Charakterisierung der osteogenen Differenzierung humaner mesenchymaler Stammzellen aus dem Knochenmark (BM-MSCs), dem Fettgewebe (AT-MSCs) und dem Nabelschnurblut (CB-MSCs) mit der humanen embryonalen Stammzelllinie hES H1 in 2D und 3D in vitro Kulturen. Weiterhin wurde evaluiert, ob man Differenzierungsprozesse mit nicht invasiven Verfahren bestimmen kann. Die Charakterisierung der Stammzellen in 2D demonstrierte ein hohes osteogenes Potential i...

  7. Puhkuse ootel / Kristjan Port

    Index Scriptorium Estoniae

    Port, Kristjan

    2007-01-01

    Peagi 125. sünnipäeva pidavas ameerika naisteajakirjas "The Ladies Home Journal" 1990. aasta ühes numbris kirjeldatakse elu saja aasta pärast ehk täna. Toonane ennustus koos mõne mööndusega paistab üllatavalt tõetruu. Enamik neist optimismist kantud visioonidest kõnelevad soovist vähendada tööd ja vaeva ning suurendada meelehead

  8. Energy Referencing in LANL HE-EOS Codes

    Energy Technology Data Exchange (ETDEWEB)

    Leiding, Jeffery Allen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Coe, Joshua Damon [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-10-19

    Here, We briefly describe the choice of energy referencing in LANL's HE-EOS codes, HEOS and MAGPIE. Understanding this is essential to comparing energies produced by different EOS codes, as well as to the correct calculation of shock Hugoniots of HEs and other materials. In all equations after (3) throughout this report, all energies, enthalpies and volumes are assumed to be molar quantities.

  9. Carbohydrate plasma expanders for passive tumor targeting

    DEFF Research Database (Denmark)

    Hoffmann, Stefan; Caysa, Henrike; Kuntsche, Judith

    2013-01-01

    The objective of this study was to investigate the suitability of carbohydrate plasma volume expanders as a novel polymer platform for tumor targeting. Many synthetic polymers have already been synthesized for targeted tumor therapy, but potential advantages of these carbohydrates include inexpen...... was characterized in human colon carcinoma xenograft bearing nude mice. A tumor specific accumulation of HES 450 was observed, which proves it’s potential as carrier for passive tumor targeting....

  10. [Expression of Jagged1 mRNA in human epithelial ovarian carcinoma tissues and effect of RNA interference of Jagged1 on growth of xenograft in nude mice].

    Science.gov (United States)

    Liu, G Y; Gao, Z H; Li, L; Song, T T; Sheng, X G

    2016-06-25

    To investigate the expression of Jagged1 in human epithelial ovarian carcinoma tissues and the effect of Jagged1 on growth of xenograft in nude mice. (1) Forty-eight cases of ovarian cancer and 30 cases of patients with benign epithelial ovarian tumor in the Henan Province Xinxiang Central Hospital during Feb. 2011 to Mar. 2014 were enrolled in this study. The mRNA expression of Jagged1, Notch1 and the downstream target genes Hes1, Hey1 were analyzed by using realtime PCR method. (2) The ovarian cancer xenograft models in nude mice were constructed by injecting SKOV3 cells in axillary subcutaneouswere. The nude mice were randomly divided into Jagged1 interference group, blank plasmid group and control group. Each group had 10 mice. They were transfected with pcDNA3.1(+)-siRNA-Jagged1, blank plasmid pDC3.1 and phosphate buffer, respectively. The tumor volumes and tumor masses were measured 14 days after transfection and the inhibition rate was calculated. The relative mRNA expression of Jagged1, Notch1, Hes1 and Hey1 in xenograft tissues after transfection in each group was detected by using realtime PCR technique and the relative protein expression of Jagged1, Notch1, Hes1 and Hey1 in xenograft tissues was detected by utilizing western blot method. (1) The relative mRNA expression of Jagged1, Notch1, Hes1 and Hey1 in ovarian cancer tissues were higher than benign ovarian tumor tissues, the differences were statistically significant (Ptissues of nude micein Jagged1 interference group were lower than that in the other two groups, the differences were statistically significant (Ptissues of nude mice among the three groups (P>0.05). Jagged1 is highly expressed in epithelial ovarian carcinoma. Jagged1 gene interference in xenograft tumor can inhibit ovarian cancer cell growth and improve tumor suppressor rate, which probably play roles by inhibiting Notch1 signaling pathway.

  11. Dynamic performance analysis of two regional Nuclear Hybrid Energy Systems

    International Nuclear Information System (INIS)

    Garcia, Humberto E.; Chen, Jun; Kim, Jong S.; Vilim, Richard B.; Binder, William R.; Bragg Sitton, Shannon M.; Boardman, Richard D.; McKellar, Michael G.; Paredis, Christiaan J.J.

    2016-01-01

    In support of more efficient utilization of clean energy generation sources, including renewable and nuclear options, HES (hybrid energy systems) can be designed and operated as FER (flexible energy resources) to meet both electrical and thermal energy needs in the electric grid and industrial sectors. These conceptual systems could effectively and economically be utilized, for example, to manage the increasing levels of dynamic variability and uncertainty introduced by VER (variable energy resources) such as renewable sources (e.g., wind, solar), distributed energy resources, demand response schemes, and modern energy demands (e.g., electric vehicles) with their ever changing usage patterns. HES typically integrate multiple energy inputs (e.g., nuclear and renewable generation) and multiple energy outputs (e.g., electricity, gasoline, fresh water) using complementary energy conversion processes. This paper reports a dynamic analysis of two realistic HES including a nuclear reactor as the main baseload heat generator and to assess the local (e.g., HES owners) and system (e.g., the electric grid) benefits attainable by their application in scenarios with multiple commodity production and high renewable penetration. It is performed for regional cases – not generic examples – based on available resources, existing infrastructure, and markets within the selected regions. This study also briefly addresses the computational capabilities developed to conduct such analyses. - Highlights: • Hybrids including renewables can operate as dispatchable flexible energy resources. • Nuclear energy can address high variability and uncertainty in energy systems. • Nuclear hybrids can reliably provide grid services over various time horizons. • Nuclear energy can provide operating reserves and grid inertia under high renewables. • Nuclear hybrids can greatly reduce GHG emissions and support grid and industry needs.

  12. Kunst ja glamuur ulatavad käe / Jaak Lõhmus

    Index Scriptorium Estoniae

    Lõhmus, Jaak

    2005-01-01

    Cannes'i 58. filmifestival toimub 11.-22. maini. Võistluskavast, esilinastustest (George Lucas'e "Tähesõdade" värske osa) ja žüriist, mida juhib Emir Kusturica. "Režissööride kahe nädala" kavas on ka leedulase Šarunas Bartase "Seitse nähtamatut meest". Võistluskava 20 filmi hulgas pole dokumentaal- ja animafilme

  13. Cancer recording in patients with and without type 2 diabetes in the Clinical Practice Research Datalink primary care data and linked hospital admission data: a cohort study.

    Science.gov (United States)

    Williams, Rachael; van Staa, Tjeerd-Pieter; Gallagher, Arlene M; Hammad, Tarek; Leufkens, Hubert G M; de Vries, Frank

    2018-05-26

    Conflicting results from studies using electronic health records to evaluate the associations between type 2 diabetes and cancer fuel concerns regarding potential biases. This study aimed to describe completeness of cancer recording in UK primary care data linked to hospital admissions records. Patients aged 40+ years with insulin or oral antidiabetic prescriptions in Clinical Practice Research Datalink (CPRD) primary care without type 1 diabetes were matched by age, sex and general practitioner practice to non-diabetics. Those eligible for linkage to Hospital Episode Statistics Admitted Patient Care (HES APC), and with follow-up during April 1997-December 2006 were included. Cancer recording and date of first record of cancer were compared. Characteristics of patients with cancer most likely to have the diagnosis recorded only in a single data source were assessed. Relative rates of cancer estimated from the two datasets were compared. 53 585 patients with type 2 diabetes matched to 47 435 patients without diabetes were included. Of all cancers (excluding non-melanoma skin cancer) recorded in CPRD, 83% were recorded in HES APC. 94% of cases in HES APC were recorded in CPRD. Concordance was lower when restricted to same-site cancer records, and was negatively associated with increasing age. Relative rates for cancer were similar in both datasets. Good concordance in cancer recording was found between CPRD and HES APC among type 2 diabetics and matched controls. Linked data may reduce misclassification and increase case ascertainment when analysis focuses on site-specific cancers. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  14. Comparison of dementia recorded in routinely collected hospital admission data in England with dementia recorded in primary care.

    Science.gov (United States)

    Brown, Anna; Kirichek, Oksana; Balkwill, Angela; Reeves, Gillian; Beral, Valerie; Sudlow, Cathie; Gallacher, John; Green, Jane

    2016-01-01

    Electronic linkage of UK cohorts to routinely collected National Health Service (NHS) records provides virtually complete follow-up for cause-specific hospital admissions and deaths. The reliability of dementia diagnoses recorded in NHS hospital data is not well documented. For a sample of Million Women Study participants in England we compared dementia recorded in routinely collected NHS hospital data (Hospital Episode Statistics: HES) with dementia recorded in two separate sources of primary care information: a primary care database [Clinical Practice Research Datalink (CPRD), n = 340] and a survey of study participants' General Practitioners (GPs, n = 244). Dementia recorded in HES fully agreed both with CPRD and with GP survey data for 85% of women; it did not agree for 1 and 4%, respectively. Agreement was uncertain for the remaining 14 and 11%, respectively; and among those classified as having uncertain agreement in CPRD, non-specific terms compatible with dementia, such as 'memory loss', were recorded in the CPRD database for 79% of the women. Agreement was significantly better (p primary care (CPRD) than in hospital (HES) data. Age-specific rates for dementia based on the hospital admission data were lower than the rates based on the primary care data, but were similar if the delay in recording in HES was taken into account. Dementia recorded in routinely collected NHS hospital admission data for women in England agrees well with primary care records of dementia assessed separately from two different sources, and is sufficiently reliable for epidemiological research.

  15. Agreement between photographic screening and hospital biomicroscopy grading of diabetic retinopathy and maculopathy.

    Science.gov (United States)

    Healy, Rachel; Sallam, Ahmed; Jones, Vanessa; Donachie, Paul H J; Scanlon, Peter H; Stratton, Irene M; Johnston, Robert L

    2014-01-01

    To examine the level of agreement and reasons for disagreement between grading of diabetic retinopathy and maculopathy using mydriatic digital photographs in a diabetic retinopathy screening service (DRSS) and hospital eye service (HES). English NHS Diabetic Eye Screening Programme grades for diabetic retinopathy prospectively recorded on a hospital electronic medical record were compared to the grades from the DRSS event that prompted referral. In cases of disagreement, images were reviewed. Data for 1,501 patients (3,002 eyes) referred between 2008 and 2011 were analyzed. The HES retinopathy grades were R0 (no retinopathy) in 341 eyes, R1 (background retinopathy) in 1,712 eyes, R2 (pre-proliferative retinopathy) in 821 eyes, and R3 (proliferative retinopathy) in 128 eyes. The DRSS grades were in agreement in 2,309 eyes (76.9%), recorded a lower grade in 227 eyes, and recorded a higher grade in 466 eyes. Agreement was substantial (κ = 0.65). The commonest cause for disagreement was overgrading of R1 as R2 by hospital clinicians. The HES maculopathy grades were M0 (no maculopathy) in 2,267 eyes and M1 (maculopathy) in 735 eyes. The DRSS were in agreement in 2,111 eyes (70.2%), recorded a lower grade in 106 eyes, and recorded a higher grade in 785 eyes. Agreement was fair (κ = 0.39). The commonest cause for disagreement was hospital clinicians missing fine exudates. This study establishes a benchmark standard for agreement between HES and DRSS grading. Review of DRSS and grading reports images for newly referred patients is likely to improve levels of agreement, particularly for diabetic retinopathy, and should be strongly encouraged.

  16. ЕBSCO Discovery Service – ключов инструмент за информационно обслужване в Нов бълг арски университет=ЕBSCO Discovery Service – Key Tool for Information Service at New Bulgarian University

    OpenAIRE

    Todorova, Radostina

    2016-01-01

    Academic libraries deliver to their patrons data bases of different type and structure. At the beginning, the files are on optical disks, but nowadays they are online available. Now a varied content of books, articles, newspapers, images, audio and video files hes been offered and almost each library has more than one information resourse. This variety of electronic information resourses puts both librarians and patrons to test. Students and lecturers often have difficulty in using the electr...

  17. Influence of laser induced hot electrons on the threshold for shock ignition of fusion reactions

    Energy Technology Data Exchange (ETDEWEB)

    Colaïtis, A.; Ribeyre, X.; Le Bel, E.; Duchateau, G.; Nicolaï, Ph.; Tikhonchuk, V. [Centre Lasers Intenses et Applications, Université de Bordeaux - CNRS - CEA, UMR 5107,351 Cours de la Libération, 33400 Talence (France)

    2016-07-15

    The effects of Hot Electrons (HEs) generated by the nonlinear Laser-Plasma Interaction (LPI) on the dynamics of Shock Ignition Inertial Confinement Fusion targets are investigated. The coupling between the laser beam, plasma dynamics and hot electron generation and propagation is described with a radiative hydrodynamics code using an inline model based on Paraxial Complex Geometrical Optics [Colaïtis et al., Phys. Rev. E 92, 041101 (2015)]. Two targets are considered: the pure-DT HiPER target and a CH-DT design with baseline spike powers of the order of 200–300 TW. In both cases, accounting for the LPI-generated HEs leads to non-igniting targets when using the baseline spike powers. While HEs are found to increase the ignitor shock pressure, they also preheat the bulk of the imploding shell, notably causing its expansion and contamination of the hotspot with the dense shell material before the time of shock convergence. The associated increase in hotspot mass (i) increases the ignitor shock pressure required to ignite the fusion reactions and (ii) significantly increases the power losses through Bremsstrahlung X-ray radiation, thus rapidly cooling the hotspot. These effects are less prominent for the CH-DT target where the plastic ablator shields the lower energy LPI-HE spectrum. Simulations using higher laser spike powers of 500 TW suggest that the CH-DT capsule marginally ignites, with an ignition window width significantly smaller than without LPI-HEs, and with three quarters of the baseline target yield. The latter effect arises from the relation between the shock launching time and the shell areal density, which becomes relevant in presence of a LPI-HE preheating.

  18. Immunohistochemical expression of Notch signaling in the lining epithelium of periapical cysts.

    Science.gov (United States)

    Meliou, Eleni; Kerezoudis, Nikolaos; Tosios, Konstantinos; Lafkas, Daniel; Kiaris, Hippokratis

    2011-02-01

    In this study we evaluated the immunohistochemical expression of the receptors Notch 1 and Notch 2, the ligand Delta 1, and the transcription factors HES 1 and HES 5 in the epithelium of well-defined periapical cysts. Immunohistochemistry was carried out on 55 formalin-fixed and paraffin-embedded, well-defined periapical cysts with minimum inflammation, obtained from the archival tissue database of the Department of Oral Pathology and Surgery. Western blotting was performed to evaluate the specificity of the anti-Notch antibody and the expression of Notch signaling in 5 fresh-frozen periapical cysts. The levels of staining intensity were estimated by the performance of a semiautomated image analysis system. Descriptive statistic of mean values obtained by computerized image analysis method was performed. Immunostaining reaction of all Notch signaling components was observed in the cytoplasm and/or the cytoplasmic membrane in the majority of epithelial cells of periapical cysts. Nuclear staining was observed occasionally in all cases. Notch 2 showed strong staining in 52.83% of the cases, followed by Notch 1 (35.85%), HES 1 and HES 5 moderate staining in 72.73% and 57.69% of the cases, respectively, and Delta 1 weak staining in 58.33% of the cases. No statistical correlation was found between the antibodies and the sex or the age of the study group. Notch is an evolutionarily conserved signaling mechanism that regulates cell fate decisions during development and postnatal life in organisms as diverse as worms, flies, and humans. The present observations indicate that Notch pathway is active downstream in the lining epithelium of periapical cysts, suggesting an involvement of this pathway in periapical cyst growth and expansion. Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  19. Highly excited strings I: Generating function

    Directory of Open Access Journals (Sweden)

    Dimitri P. Skliros

    2017-03-01

    Full Text Available This is the first of a series of detailed papers on string amplitudes with highly excited strings (HES. In the present paper we construct a generating function for string amplitudes with generic HES vertex operators using a fixed-loop momentum formalism. We generalise the proof of the chiral splitting theorem of D'Hoker and Phong to string amplitudes with arbitrary HES vertex operators (with generic KK and winding charges, polarisation tensors and oscillators in general toroidal compactifications E=RD−1,1×TDcr−D (with generic constant Kähler and complex structure target space moduli, background Kaluza–Klein (KK gauge fields and torsion. We adopt a novel approach that does not rely on a “reverse engineering” method to make explicit the loop momenta, thus avoiding a certain ambiguity pointed out in a recent paper by Sen, while also keeping the genus of the worldsheet generic. This approach will also be useful in discussions of quantum gravity and in particular in relation to black holes in string theory, non-locality and breakdown of local effective field theory, as well as in discussions of cosmic superstrings and their phenomenological relevance. We also discuss the manifestation of wave/particle (or rather wave/string duality in string theory.

  20. Germline excision of transgenes in Aedes aegypti by homing endonucleases.

    Science.gov (United States)

    Aryan, Azadeh; Anderson, Michelle A E; Myles, Kevin M; Adelman, Zach N

    2013-01-01

    Aedes (Ae.) aegypti is the primary vector for dengue viruses (serotypes1-4) and chikungunya virus. Homing endonucleases (HEs) are ancient selfish elements that catalyze double-stranded DNA breaks (DSB) in a highly specific manner. In this report, we show that the HEs Y2-I-AniI, I-CreI and I-SceI are all capable of catalyzing the excision of genomic segments from the Ae. aegypti genome in a heritable manner. Y2-I-AniI demonstrated the highest efficiency at two independent genomic targets, with 20-40% of Y2-I-AniI-treated individuals producing offspring that had lost the target transgene. HE-induced DSBs were found to be repaired via the single-strand annealing (SSA) and non-homologous end-joining (NHEJ) pathways in a manner dependent on the availability of direct repeat sequences in the transgene. These results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, and confirm the utility of HEs in the manipulation and modification of transgenes in this important vector.

  1. dlk acts as a negative regulator of Notch1 activation through interactions with specific EGF-like repeats

    International Nuclear Information System (INIS)

    Baladron, Victoriano; Ruiz-Hidalgo, Maria Jose; Nueda, Maria Luisa; Diaz-Guerra, Maria Jose M.; Garcia-Ramirez, Jose Javier; Bonvini, Ezio; Gubina, Elena; Laborda, Jorge

    2005-01-01

    The protein dlk, encoded by the Dlk1 gene, belongs to the Notch epidermal growth factor (EGF)-like family of receptors and ligands, which participate in cell fate decisions during development. The molecular mechanisms by which dlk regulates cell differentiation remain unknown. By using the yeast two-hybrid system, we found that dlk interacts with Notch1 in a specific manner. Moreover, by using luciferase as a reporter gene under the control of a CSL/RBP-Jk/CBF-1-dependent promoter in the dlk-negative, Notch1-positive Balb/c 14 cell line, we found that addition of synthetic dlk EGF-like peptides to the culture medium or forced expression of dlk decreases endogenous Notch activity. Furthermore, the expression of the gene Hes-1, a target for Notch1 activation, diminishes in confluent Balb/c14 cells transfected with an expression construct encoding for the extracellular EGF-like region of dlk. The expression of Dlk1 and Notch1 increases in 3T3-L1 cells maintained in a confluent state for several days, which is associated with a concomitant decrease in Hes-1 expression. On the other hand, the decrease of Dlk1 expression in 3T3-L1 cells by antisense cDNA transfection is associated with an increase in Hes-1 expression. These results suggest that dlk functionally interacts in vivo with Notch1, which may lead to the regulation of differentiation processes modulated by Notch1 activation and signaling, including adipogenesis

  2. Fibrinogen estimates are influenced by methods of measurement and hemodilution with colloid plasma expanders.

    Science.gov (United States)

    Fenger-Eriksen, Christian; Moore, Gary W; Rangarajan, Savita; Ingerslev, Jørgen; Sørensen, Benny

    2010-12-01

    Measurement of plasma fibrinogen is often required in critically ill patients or massively bleeding patients being resuscitated with colloid plasma expander. This study aimed at evaluating different assays of plasma fibrinogen after in vitro dilution with commonly used plasma expanders and challenged the hypothesis that levels of fibrinogen are estimated significantly higher in plasma diluted with colloid plasma expander compared with isotonic saline. Fibrinogen measurements were established in plasma samples each diluted in vitro to 30 or 50% with isotonic saline, hydroxyethyl starch (HES) 130/0.4, and human albumin. Fibrinogen levels were assessed using an antigen determination, three photo-optical Clauss methods, one mechanical Clauss method, a prothrombin-derived method, and viscoelastic measurement through thromboelastometry. Measurement of fibrinogen levels was significantly different when performed on alternate analytical platforms. By 30 and 50% dilution with HES 130/0.4 coagulation analyzers using the photo-optical Clauss methods significantly overestimated levels of fibrinogen. Dilution with human albumin did not affect fibrinogen levels except from one analyzer by 50% dilution level. Viscoelastic measurement of fibrin polymerization was reduced at both dilution levels and appeared to reflect the impairment of fibrin polymerization induced by HES 130/0.4 and to a lesser extent human albumin. This study demonstrated that different automated coagulation analyzers revealed significantly different levels of fibrinogen. The presence of colloid plasma expander gave rise to erroneous high levels of fibrinogen returned from some coagulation analyzers employing the method of Clauss. © 2010 American Association of Blood Banks.

  3. Effects of Acute Bleeding Followed by Hydroxyethyl Starch 130/0.4 or a Crystalloid on Propofol Concentrations, Cerebral Oxygenation, and Electroencephalographic and Haemodynamic Variables in Pigs

    Directory of Open Access Journals (Sweden)

    Aura Silva

    2014-01-01

    Full Text Available Bleeding changes the haemodynamics, compromising organ perfusion. In this study, the effects of bleeding followed by replacement with hydroxyethyl starch 130/0.4 (HES or lactated Ringer’s (LR on cerebral oxygenation and electroencephalogram-derived parameters were investigated. Twelve young pigs under propofol-remifentanil anaesthesia were bled 30 mL/kg and, after a 20-minute waiting period, volume replacement was performed with HES (GHES; N=6 or LR (GRL; N=6. Bleeding caused a decrease of more than 50% in mean arterial pressure (P<0.01 and a decrease in cerebral oximetry (P=0.039, bispectral index, and electroencephalogram total power (P=0.04 and P<0.01, resp., while propofol plasma concentrations increased (P<0.01. Both solutions restored the haemodynamics and cerebral oxygenation similarly and were accompanied by an increase in electroencephalogram total power. No differences between groups were found. However, one hour after the end of the volume replacement, the cardiac output (P=0.03 and the cerebral oxygenation (P=0.008 decreased in the GLR and were significantly lower than in GHES (P=0.02. Volume replacement with HES 130/0.4 was capable of maintaining the cardiac output and cerebral oxygenation during a longer period than LR and caused a decrease in the propofol plasma concentrations.

  4. Low antigenicity of hematopoietic progenitor cells derived from human ES cells

    Directory of Open Access Journals (Sweden)

    Eun-Mi Kim

    2010-02-01

    Full Text Available Eun-Mi Kim1, Nicholas Zavazava1,21Department of Internal Medicine, University of Iowa and Veterans Affairs Medical Center, Iowa City, Iowa, USA; 2Immunology Graduate Program, University of Iowa, Iowa City, Iowa, USAAbstract: Human embryonic stem (hES cells are essential for improved understanding of diseases and our ability to probe new therapies for use in humans. Currently, bone marrow cells and cord blood cells are used for transplantation into patients with hematopoietic malignancies, immunodeficiencies and in some cases for the treatment of autoimmune diseases. However, due to the high immunogenicity of these hematopoietic cells, toxic regimens of drugs are required for preconditioning and prevention of rejection. Here, we investigated the efficiency of deriving hematopoietic progenitor cells (HPCs from the hES cell line H13, after co-culturing with the murine stromal cell line OP9. We show that HPCs derived from the H13 ES cells poorly express major histocompatibility complex (MHC class I and no detectable class II antigens (HLA-DR. These characteristics make hES cell-derived hematopoietic cells (HPCs ideal candidates for transplantation across MHC barriers under minimal immunosuppression.Keywords: human embryonic stem cells, H13, hematopoiesis, OP9 stromal cells, immunogenicity

  5. Hawaii Energy Strategy Project 2: Fossil Energy Review. Task IV. Scenario development and analysis

    Energy Technology Data Exchange (ETDEWEB)

    Yamaguchi, N.D.; Breazeale, K. [ed.

    1993-12-01

    The Hawaii Energy Strategy (HES) Program is a seven-project effort led by the State of Hawaii Department of Business, Economic Development & Tourism (DBEDT) to investigate a wide spectrum of Hawaii energy issues. The East-West Center`s Program on Resources: Energy and Minerals, has been assigned HES Project 2, Fossil Energy Review, which focuses on fossil energy use in Hawaii and the greater regional and global markets. HES Project 2 has four parts: Task I (World and Regional Fossil Energy Dynamics) covers petroleum, natural gas, and coal in global and regional contexts, along with a discussion of energy and the environment. Task II (Fossil Energy in Hawaii) focuses more closely on fossil energy use in Hawaii: current utilization and trends, the structure of imports, possible future sources of supply, fuel substitutability, and energy security. Task III`s emphasis is Greenfield Options; that is, fossil energy sources not yet used in Hawaii. This task is divided into two sections: first, an in-depth {open_quotes}Assessment of Coal Technology Options and Implications for the State of Hawaii,{close_quotes} along with a spreadsheet analysis model, which was subcontracted to the Environmental Assessment and Information Sciences Division of Argonne National Laboratory; and second, a chapter on liquefied natural gas (LNG) in the Asia-Pacific market and the issues surrounding possible introduction of LNG into the Hawaii market.

  6. A Novel Supercapacitor/Lithium-Ion Hybrid Energy System with a Fuzzy Logic-Controlled Fast Charging and Intelligent Energy Management System

    Directory of Open Access Journals (Sweden)

    Muhammad Adil Khan

    2018-05-01

    Full Text Available The electric powered wheelchair (EPW is an essential assistive tool for people with serious injuries or disability. This manuscript describes the validation of applied research for reducing the charging time of an electric wheelchair using a hybrid electric system (HES composed of a supercapacitor (SC bank and a lithium-ion battery with a fuzzy logic controller (FLC-based fast charging system for Li-ion batteries and a fuzzy logic-based intelligent energy management system (FLIEMS for controlling the power flow within the HES. The fast charging FLC was designed to drive the voltage difference (Vd among the different cells of a multi-cell battery and the cell voltage (Vc of an individual cell. These parameters (voltage difference and cell voltage were used as input voltages to reduce the charge time and activate a bypass equalization (BPE scheme. BPE was introduced in this paper so that the battery operates within the safe voltage range. For SC/Li-ion HES, the FLIEMS presented in this paper controls the bi-directional power flow to smooth the power extracted from Li-ion batteries. Moreover, a dual active bridge isolated bidirectional DC converter (DAB-IBDC was used for power conversion. The DAB-IBDC presented in this paper has the characteristics of galvanic isolation, and high power conversion efficiency compared to the conventional converter circuits due to the reduced reverse power flow and current stresses.

  7. Head to head comparison of the formulation and stability of concentrated solutions of HESylated versus PEGylated anakinra.

    Science.gov (United States)

    Liebner, Robert; Meyer, Martin; Hey, Thomas; Winter, Gerhard; Besheer, Ahmed

    2015-02-01

    Although PEGylation of biologics is currently the gold standard for half-life extension, the technology has a number of limitations, most importantly the non-biodegradability of PEG and the extremely high viscosity at high concentrations. HESylation is a promising alternative based on coupling to the biodegradable polymer hydroxyethyl starch (HES). In this study, we are comparing HESylation with PEGylation regarding the effect on the protein's physicochemical properties, as well as on formulation at high concentrations, where protein stability and viscosity can be compromised. For this purpose, the model protein anakinra is coupled to HES or PEG by reductive amination. Results show that coupling of HES or PEG had practically no effect on the protein's secondary structure, and that it reduced protein affinity by one order of magnitude, with HESylated anakinra more affine than the PEGylated protein. The viscosity of HESylated anakinra at protein concentrations up to 75 mg/mL was approximately 40% lower than that of PEG-anakinra. Both conjugates increased the apparent melting temperature of anakinra in concentrated solutions. Finally, HESylated anakinra was superior to PEG-anakinra regarding monomer recovery after 8 weeks of storage at 40°C. These results show that HESylating anakinra offers formulation advantages compared with PEGylation, especially for concentrated protein solutions. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

  8. Predicting hepatitis B monthly incidence rates using weighted Markov chains and time series methods.

    Science.gov (United States)

    Shahdoust, Maryam; Sadeghifar, Majid; Poorolajal, Jalal; Javanrooh, Niloofar; Amini, Payam

    2015-01-01

    Hepatitis B (HB) is a major global mortality. Accurately predicting the trend of the disease can provide an appropriate view to make health policy disease prevention. This paper aimed to apply three different to predict monthly incidence rates of HB. This historical cohort study was conducted on the HB incidence data of Hamadan Province, the west of Iran, from 2004 to 2012. Weighted Markov Chain (WMC) method based on Markov chain theory and two time series models including Holt Exponential Smoothing (HES) and SARIMA were applied on the data. The results of different applied methods were compared to correct percentages of predicted incidence rates. The monthly incidence rates were clustered into two clusters as state of Markov chain. The correct predicted percentage of the first and second clusters for WMC, HES and SARIMA methods was (100, 0), (84, 67) and (79, 47) respectively. The overall incidence rate of HBV is estimated to decrease over time. The comparison of results of the three models indicated that in respect to existing seasonality trend and non-stationarity, the HES had the most accurate prediction of the incidence rates.

  9. Uptake, sequestration and tolerance of cadmium at cellular levels in the hyperaccumulator plant species Sedum alfredii

    Energy Technology Data Exchange (ETDEWEB)

    Tian, Shengke; Xie, Ruohan; Wang, Haixin; Hu, Yan; Hou, Dandi; Liao, Xingcheng; Brown, Patrick H.; Yang, Hongxia; Lin, Xianyong; Labavitch, John M.; Lu, Lingli

    2017-04-01

    Sedum alfredii is one of a few plant species known to hyperaccumulate cadmium (Cd). Uptake, localization, and tolerance of Cd at cellular levels in shoots were compared in hyperaccumulating (HE) and non-hyperaccumulating (NHE) ecotypes of Sedum alfredii. X-ray fluorescence images of Cd in stems and leaves showed only a slight Cd signal restricted within vascular bundles in the NHEs, while enhanced localization of Cd, with significant tissue- and age-dependent variations, was detected in HEs. In contrast to the vascular-enriched Cd in young stems, parenchyma cells in leaf mesophyll, stem pith and cortex tissues served as terminal storage sites for Cd sequestration in HEs. Kinetics of Cd transport into individual leaf protoplasts of the two ecotypes showed little difference in Cd accumulation. However, far more efficient storage of Cd in vacuoles was apparent in HEs. Subsequent analysis of cell viability and hydrogen peroxide levels suggested that HE protoplasts exhibited higher resistance to Cd than those of NHE protoplasts. These results suggest that efficient sequestration into vacuoles, as opposed to rapid transport into parenchyma cells, is a pivotal process in Cd accumulation and homeostasis in shoots of HE S. alfredii. This is in addition to its efficient root-to-shoot translocation of Cd.

  10. SOX1 links the function of neural patterning and Notch signalling in the ventral spinal cord during the neuron-glial fate switch

    Energy Technology Data Exchange (ETDEWEB)

    Genethliou, Nicholas; Panayiotou, Elena [The Cyprus Institute of Neurology and Genetics, Airport Avenue, No. 6, Agios Dometios, 2370 Nicosia (Cyprus); Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia (Cyprus); Panayi, Helen; Orford, Michael; Mean, Richard; Lapathitis, George; Gill, Herman; Raoof, Sahir [The Cyprus Institute of Neurology and Genetics, Airport Avenue, No. 6, Agios Dometios, 2370 Nicosia (Cyprus); Gasperi, Rita De; Elder, Gregory [James J. Peters VA Medical Center, Research and Development (3F22), 130 West Kingsbridge Road, Bronx, NY 10468 (United States); Kessaris, Nicoletta; Richardson, William D. [Wolfson Institute for Biomedical Research and Research Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT (United Kingdom); Malas, Stavros, E-mail: smalas@cing.ac.cy [The Cyprus Institute of Neurology and Genetics, Airport Avenue, No. 6, Agios Dometios, 2370 Nicosia (Cyprus); Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia (Cyprus)

    2009-12-25

    During neural development the transition from neurogenesis to gliogenesis, known as the neuron-glial ({Nu}/G) fate switch, requires the coordinated function of patterning factors, pro-glial factors and Notch signalling. How this process is coordinated in the embryonic spinal cord is poorly understood. Here, we demonstrate that during the N/G fate switch in the ventral spinal cord (vSC) SOX1 links the function of neural patterning and Notch signalling. We show that, SOX1 expression in the vSC is regulated by PAX6, NKX2.2 and Notch signalling in a domain-specific manner. We further show that SOX1 regulates the expression of Hes1 and that loss of Sox1 leads to enhanced production of oligodendrocyte precursors from the pMN. Finally, we show that Notch signalling functions upstream of SOX1 during this fate switch and is independently required for the acquisition of the glial fate perse by regulating Nuclear Factor I A expression in a PAX6/SOX1/HES1/HES5-independent manner. These data integrate functional roles of neural patterning factors, Notch signalling and SOX1 during gliogenesis.

  11. Diagnostic and therapeutic considerations in idiopathic hypereosinophilia with warm autoimmune hemolytic anemia

    Directory of Open Access Journals (Sweden)

    Sweidan AJ

    2015-09-01

    Full Text Available Alexander J Sweidan,1,2 Adam K Brys,3 David D Sohn,1,2 Milan R Sheth4 1University of California Los Angeles, Los Angeles, CA, USA; 2Department of Internal Medicine, St Mary Medical Center, Long Beach, CA, USA; 3School of Medicine, Duke University, Durham, NC, USA; 4Long Beach Memorial Hospital, Long Beach, CA, USA Abstract: Hypereosinophilic syndrome (HES encompasses numerous diverse conditions resulting in peripheral hypereosinophilia that cannot be explained by hypersensitivity, infection, or atopy and that is not associated with known systemic diseases with specific organ involvement. HES is often attributed to neoplastic or reactive causes, such as chronic eosinophilic leukemia, although a majority of cases remains unexplained and are considered idiopathic. Here, we review the current diagnosis and management of HES and present a unique case of profound hypereosinophilia associated with warm autoimmune hemolytic anemia requiring intensive management. This case clearly illustrates the limitations of current knowledge with respect to hypereosinophilia syndrome as well as the challenges associated with its classification and management. Keywords: hypereosinophilia, eosinophils, myeloproliferative disorder, autoimmune hemolytic anemia, idiopathic autoimmune hemolytic anemia, leukemia

  12. Hepatitis B surface antigen incorporated in dissolvable microneedle array patch is antigenic and thermostable.

    Science.gov (United States)

    Poirier, Danielle; Renaud, Frédéric; Dewar, Vincent; Strodiot, Laurent; Wauters, Florence; Janimak, Jim; Shimada, Toshio; Nomura, Tatsuya; Kabata, Koki; Kuruma, Koji; Kusano, Takayuki; Sakai, Masaki; Nagasaki, Hideo; Oyamada, Takayoshi

    2017-11-01

    Alternatives to syringe-based administration are considered for vaccines. Intradermal vaccination with dissolvable microneedle arrays (MNA) appears promising in this respect, as an easy-to-use and painless method. In this work, we have developed an MNA patch (MNAP) made of hydroxyethyl starch (HES) and chondroitin sulphate (CS). In swines, hepatitis B surface antigen (HBsAg) formulated with the saponin QS-21 as adjuvant, both incorporated in HES-based MNAP, demonstrated the same level of immunogenicity as a commercially available aluminum-adjuvanted HBsAg vaccine, after two immunizations 28 days apart. MNAP application was associated with transient skin reactions (erythema, lump, scab), particularly evident when the antigen was delivered with the adjuvant. The thermostability of the adjuvanted antigen when incorporated in the HES-based matrix was also assessed by storing MNAP at 37, 45 or 50 °C for up to 6 months. We could demonstrate that antigenicity was retained at 37 and 45 °C and only a 10% loss was observed after 6 months at 50 °C. Our results are supportive of MNAP as an attractive alternative to classical syringe-based vaccination. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  13. Changes in colloid solution sales in Nordic countries.

    Science.gov (United States)

    Kongsgaard, U E; Holtan, A; Perner, A

    2018-04-01

    Administration of resuscitation fluid is a common intervention in the treatment of critically ill patients, but the right choice of fluid is still a matter of debate. Changes in medical practice are based on new evidence and guidelines as well as traditions and personal preferences. Official warnings against the use of hydroxyl-ethyl-starch (HES) solutions have been issued. Nordic guidelines have issued several strong recommendations favouring crystalloids over colloids in all patient groups. Our objective was to describe the patterns of colloid use in Nordic countries from 2012 to 2016. The data were obtained from companies that provide pharmaceutical statistics in different countries. The data are sales figures from pharmaceutical companies to pharmacies and health institutions. We found a 56% reduction in the total sales of all colloids in Nordic countries over a 5-year period. These findings were mainly related to a 92% reduction in the sales of HES solutions. A reduction in sales of other synthetic colloids has also occurred. During the same period, we found a 46% increase in albumin sales, but these numbers varied between Nordic countries. The general reduction in colloid sales likely reflects the recommendation that colloids should be used only in special circumstances. The dramatic reduction in the sales of HES solutions was expected given evidence of harm and the official warnings. The steady increase in albumin sales and the notable differences between the five Nordic countries cannot be explained. © 2018 The Acta Anaesthesiologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

  14. Status Report on Modelling and Simulation Capabilities for Nuclear-Renewable Hybrid Energy Systems

    Energy Technology Data Exchange (ETDEWEB)

    Rabiti, C. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Epiney, A. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Talbot, P. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Kim, J. S. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bragg-Sitton, S. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Alfonsi, A. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Yigitoglu, A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Greenwood, S. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Cetiner, S. M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Ganda, F. [Argonne National Lab. (ANL), Argonne, IL (United States); Maronati, G. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2017-09-01

    This report summarizes the current status of the modeling and simulation capabilities developed for the economic assessment of Nuclear-Renewable Hybrid Energy Systems (N-R HES). The increasing penetration of variable renewables is altering the profile of the net demand, with which the other generators on the grid have to cope. N-R HES analyses are being conducted to determine the potential feasibility of mitigating the resultant volatility in the net electricity demand by adding industrial processes that utilize either thermal or electrical energy as stabilizing loads. This coordination of energy generators and users is proposed to mitigate the increase in electricity cost and cost volatility through the production of a saleable commodity. Overall, the financial performance of a system that is comprised of peaking units (i.e. gas turbine), baseload supply (i.e. nuclear power plant), and an industrial process (e.g. hydrogen plant) should be optimized under the constraint of satisfying an electricity demand profile with a certain level of variable renewable (wind) penetration. The optimization should entail both the sizing of the components/subsystems that comprise the system and the optimal dispatch strategy (output at any given moment in time from the different subsystems). Some of the capabilities here described have been reported separately in [1, 2, 3]. The purpose of this report is to provide an update on the improvement and extension of those capabilities and to illustrate their integrated application in the economic assessment of N-R HES.

  15. Method-Based Higher Education in Sustainability: The Potential of the Scenario Method

    Directory of Open Access Journals (Sweden)

    Richard Beecroft

    2014-05-01

    Full Text Available Both sustainability and education are challenging process-oriented objectives. When the aim is to combine both notions, as in Higher Education in Sustainability (HES, it is indispensable to first establish a common ground between them. In this paper, we characterise this common ground in terms of four aspects: future orientation, normativity, global perspective, and theory engaged in practice. Based on an analysis of the common ground, one method that is well-established in a wide range of sustainability sciences shows high potential for use in HES because it covers all four aspects in detail: the scenario method. We argue that a didactical reconstruction of the scenario method is necessary to utilise its potential and develop adequate forms of teaching in higher education. The scenario method is used to construct and analyse a set of alternative future developments to support decisions that have to be made in the present. Didactical reconstruction reveals a spectrum of objectives for which the scenario method can be employed: (1 projection; (2 teleological planning and (3 an explorative search for possibilities not yet considered. By studying and experimenting with this spectrum of objectives, students in HES can develop fundamental reflexive competencies in addressing the future in different ways that are relevant for both sustainability and education.

  16. Multi-Objective Optimal Design of Stand-Alone Hybrid Energy System Using Entropy Weight Method Based on HOMER

    Directory of Open Access Journals (Sweden)

    Jiaxin Lu

    2017-10-01

    Full Text Available Implementation of hybrid energy system (HES is generally considered as a promising way to satisfy the electrification requirements for remote areas. In the present study, a novel decision making methodology is proposed to identify the best compromise configuration of HES from a set of feasible combinations obtained from HOMER. For this purpose, a multi-objective function, which comprises four crucial and representative indices, is formulated by applying the weighted sum method. The entropy weight method is employed as a quantitative methodology for weighting factors calculation to enhance the objectivity of decision-making. Moreover, the optimal design of a stand-alone PV/wind/battery/diesel HES in Yongxing Island, China, is conducted as a case study to validate the effectiveness of the proposed method. Both the simulation and optimization results indicate that, the optimization method is able to identify the best trade-off configuration among system reliability, economy, practicability and environmental sustainability. Several useful conclusions are given by analyzing the operation of the best configuration.

  17. Validation of a survey instrument to assess home environments for physical activity and healthy eating in overweight children

    Directory of Open Access Journals (Sweden)

    Crane Lori A

    2008-01-01

    Full Text Available Abstract Background Few measures exist to measure the overall home environment for its ability to support physical activity (PA and healthy eating in overweight children. The purpose of this study was to develop and test the reliability and validity of such a measure. Methods The Home Environment Survey (HES was developed to reflect availability, accessibility, parental role modelling, and parental policies related to PA resources, fruits and vegetables (F&V, and sugar sweetened drinks and snacks (SS. Parents of overweight children (n = 219 completed the HES and concurrent behavioural assessments. Children completed the Block Kids survey and wore an accelerometer for one week. A subset of parents (n = 156 completed the HES a second time to determine test-retest reliability. Finally, 41 parent dyads living in the same home (n = 41 completed the survey to determine inter-rater reliability. Initial psychometric analyses were completed to trim items from the measure based on lack of variability in responses, moderate or higher item to scale correlation, or contribution to strong internal consistency. Inter-rater and test-retest reliability were completed using intraclass correlation coefficients. Validity was assessed using Pearson correlations between the HES scores and child and parent nutrition and PA. Results Eight items were removed and acceptable internal consistency was documented for all scales (α = .66–84 with the exception of the F&V accessibility. The F&V accessibility was reduced to a single item because the other two items did not meet reliability standards. Test-retest reliability was high (r > .75 for all scales. Inter-rater reliability varied across scales (r = .22–.89. PA accessibility, parent role modelling, and parental policies were all related significantly to child (r = .14–.21 and parent (r = .15–.31 PA. Similarly, availability of F&V and SS, parental role modelling, and parental policies were related to child (r

  18. Linkage of Maternity Hospital Episode Statistics data to birth registration and notification records for births in England 2005-2014: Quality assurance of linkage of routine data for singleton and multiple births.

    Science.gov (United States)

    Harper, Gillian

    2018-03-01

    To quality assure a Trusted Third Party linked data set to prepare it for analysis. Birth registration and notification records from the Office for National Statistics for all births in England 2005-2014 linked to Maternity Hospital Episode Statistics (HES) delivery records by NHS Digital using mothers' identifiers. All 6 676 912 births that occurred in England from 1 January 2005 to 31 December 2014. Every link between a registered birth and an HES delivery record for the study period was categorised as either the same baby or a different baby to the same mother, or as a wrong link, by comparing common baby data items and valid values in key fields with stepwise deterministic rules. Rates of preserved and discarded links were calculated and which features were more common in each group were assessed. Ninety-eight per cent of births originally linked to HES were left with one preserved link. The majority of discarded links were due to duplicate HES delivery records. Of the 4854 discarded links categorised as wrong links, clerical checks found 85% were false-positives links, 13% were quality assurance false negatives and 2% were undeterminable. Births linked using a less reliable stage of the linkage algorithm, births at home and in the London region, and with birth weight or gestational age values missing in HES were more likely to have all links discarded. Linkage error, data quality issues, and false negatives in the quality assurance procedure were uncovered. The procedure could be improved by allowing for transposition in date fields, and more discrimination between missing and differing values. The availability of identifiers in the datasets supported clerical checking. Other research using Trusted Third Party linkage should not assume the linked dataset is error-free or optimised for their analysis, and allow sufficient resources for this. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved

  19. An investigation of patterns in hemodynamic data indicative of impending hypotension in intensive care

    Directory of Open Access Journals (Sweden)

    Lee Joon

    2010-10-01

    Full Text Available Abstract Background In the intensive care unit (ICU, clinical staff must stay vigilant to promptly detect and treat hypotensive episodes (HEs. Given the stressful context of busy ICUs, an automated hypotensive risk stratifier can help ICU clinicians focus care and resources by prospectively identifying patients at increased risk of impending HEs. The objective of this study was to investigate the possible existence of discriminatory patterns in hemodynamic data that can be indicative of future hypotensive risk. Methods Given the complexity and heterogeneity of ICU data, a machine learning approach was used in this study. Time series of minute-by-minute measures of mean arterial blood pressure, heart rate, pulse pressure, and relative cardiac output from 1,311 records from the MIMIC II Database were used. An HE was defined as a 30-minute period during which the mean arterial pressure was below 60 mmHg for at least 90% of the time. Features extracted from the hemodynamic data during an observation period of either 30 or 60 minutes were analyzed to predict the occurrence of HEs 1 or 2 hours into the future. Artificial neural networks (ANNs were trained for binary classification (normotensive vs. hypotensive and regression (estimation of future mean blood pressure. Results The ANNs were successfully trained to discriminate patterns in the multidimensional hemodynamic data that were predictive of future HEs. The best overall binary classification performance resulted in a mean area under ROC curve of 0.918, a sensitivity of 0.826, and a specificity of 0.859. Predicting further into the future resulted in poorer performance, whereas observation duration minimally affected performance. The low prevalence of HEs led to poor positive predictive values. In regression, the best mean absolute error was 9.67%. Conclusions The promising pattern recognition performance demonstrates the existence of discriminatory patterns in hemodynamic data that can indicate

  20. In vitro optical detection of simulated blood pulse in a human tooth pulp model.

    Science.gov (United States)

    Niklas, A; Hiller, K-A; Jaeger, A; Brandt, M; Putzger, J; Ermer, C; Schulz, I; Monkman, G; Giglberger, S; Hirmer, M; Danilov, S; Ganichev, S; Schmalz, G

    2014-01-01

    Noninvasive optical methods such as photoplethysmography, established for blood pulse detection in organs, have been proposed for vitality testing of human dental pulp. However, no information is available on the mechanism of action in a closed pulp chamber and on the impairing influence of other than pulpal blood flow sources. Therefore, the aim of the present in vitro study was to develop a device for the optical detection of pulpal blood pulse and to investigate the influence of different parameters (including gingival blood flow [GBF] simulation) on the derived signals. Air, Millipore water, human erythrocyte suspensions (HES), non-particulate hemoglobin suspension (NPHS), and lysed hemoglobin suspension (LHES) were pulsed through a flexible (silicone) or a rigid (glass) tube placed within an extracted human molar in a tooth-gingiva model. HES was additionally pulsed through a rigid tube around the tooth, simulating GBF alone or combined with the flow through the tooth by two separate peristaltic pumps. Light from high-power light-emitting diodes (625 nm (red) and 940 nm (infrared [IR]); Golden Dragon, Osram, Germany) was introduced to the coronal/buccal part of the tooth, and the signal amplitude [∆U, in volts] of transmitted light was detected by a sensor at the opposite side of the tooth. Signal processing was carried out by means of a newly developed blood pulse detector. Finally, experiments were repeated with the application of rubber dam (blue, purple, pink, and black), aluminum foil, and black antistatic plastic foil. Nonparametric statistical analysis was applied (n = 5; α = 0.05). Signals were obtained for HES and LHES, but not with air, Millipore water, or NPHS. Using a flexible tube, signals for HES were higher for IR compared to red light, whereas for the rigid tube, the signals were significantly higher for red light than for IR. In general, significantly less signal amplitude was recorded for HES with the rigid glass tube than with the

  1. Status on the Component Models Developed in the Modelica Framework: High-Temperature Steam Electrolysis Plant & Gas Turbine Power Plant

    Energy Technology Data Exchange (ETDEWEB)

    Suk Kim, Jong [Idaho National Lab. (INL), Idaho Falls, ID (United States); McKellar, Michael [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bragg-Sitton, Shannon M. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Boardman, Richard D. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-10-01

    This report has been prepared as part of an effort to design and build a modeling and simulation (M&S) framework to assess the economic viability of a nuclear-renewable hybrid energy system (N-R HES). In order to facilitate dynamic M&S of such an integrated system, research groups in multiple national laboratories have been developing various subsystems as dynamic physics-based components using the Modelica programming language. In fiscal year (FY) 2015, Idaho National Laboratory (INL) performed a dynamic analysis of two region-specific N-R HES configurations, including the gas-to-liquid (natural gas to Fischer-Tropsch synthetic fuel) and brackish water reverse osmosis desalination plants as industrial processes. In FY 2016, INL has developed two additional subsystems in the Modelica framework: a high-temperature steam electrolysis (HTSE) plant and a gas turbine power plant (GTPP). HTSE has been proposed as a high priority industrial process to be integrated with a light water reactor (LWR) in an N-R HES. This integrated energy system would be capable of dynamically apportioning thermal and electrical energy (1) to provide responsive generation to the power grid and (2) to produce alternative industrial products (i.e., hydrogen and oxygen) without generating any greenhouse gases. A dynamic performance analysis of the LWR/HTSE integration case was carried out to evaluate the technical feasibility (load-following capability) and safety of such a system operating under highly variable conditions requiring flexible output. To support the dynamic analysis, the detailed dynamic model and control design of the HTSE process, which employs solid oxide electrolysis cells, have been developed to predict the process behavior over a large range of operating conditions. As first-generation N-R HES technology will be based on LWRs, which provide thermal energy at a relatively low temperature, complementary temperature-boosting technology was suggested for integration with the

  2. Fucosylation Deficiency in Mice Leads to Colitis and Adenocarcinoma

    Science.gov (United States)

    Wang, Yiwei; Huang, Dan; Chen, Kai-Yuan; Cui, Min; Wang, Weihuan; Huang, Xiaoran; Awadellah, Amad; Li, Qing; Friedman, Ann; Xin, William W.; Di Martino, Luca; Cominelli, Fabio; Miron, Alex; Chan, Ricky; Fox, James; Xu, Yan; Shen, Xiling; Kalady, Mathew F.; Markowitz, Sanford; Maillard, Ivan; Lowe, John B.; Xin, Wei; Zhou, Lan

    2016-01-01

    Background & Aims De novo synthesis of GDP-fucose, a substrate for fucosylglycans, requires sequential reactions mediated by GDP-mannose 4,6-dehydratase (GMDS) and GDP-4-keto-6-deoxymannose 3,5-epimerase-4-reductase (FX or TSTA3). GMDS deletions and mutations are found in 6%–13% of colorectal cancers; these mostly affect ascending and transverse colon. We investigated whether lack of fucosylation consequent to loss of GDP-fucose synthesis contributes to colon carcinogenesis. Methods FX deficiency and GMDS deletion produce the same biochemical phenotype of GDP-fucose deficiency. We studied a mouse model of fucosylation deficiency (Fx–/– mice) and mice with the full-length Fx gene (controls). Mice were placed on standard chow or fucose-containing diet (equivalent to a control fucosylglycan phenotype). Colon tissues were collected and analyzed histologically or by ELISAs to measure cytokine levels; T cells were also collected and analyzed. Fecal samples were analyzed by 16s rRNA sequencing. Mucosal barrier function was measured by uptake of fluorescent dextran. We transplanted bone marrow cells from Fx–/– or control mice (Ly5.2) into irradiated 8-week old Fx–/– or control mice (Ly5.1). We performed immunohistochemical analyses for expression of Notch and the hes family bHLH transcription factor (HES1) in colon tissues from mice and a panel of 60 human colorectal cancer specimens (27 left-sided, 33 right-sided). Results Fx–/– mice developed colitis and serrated-like lesions. The intestinal pathology of Fx–/– mice was reversed by addition of fucose to the diet, which restored fucosylation via a salvage pathway. In the absence of fucosylation, dysplasia appeared and progressed to adenocarcinoma in up to 40% of mice, affecting mainly the right colon and cecum. Notch was not activated in Fx–/– mice fed standard chow, leading to decreased expression of its target Hes1. Fucosylation deficiency altered the composition of the fecal microbiota, reduced

  3. Protective perioperative strategy using a third generation hydroxyethyl starch during surgery in a murine model of liver reperfusion injury Estratégia protetora perioperatória usando um hidroxietilamido de terceira geração para expansão volêmica durante a cirurgia em modelo murino de lesão de reperfusão hepática

    Directory of Open Access Journals (Sweden)

    Dora Catré

    2011-12-01

    Full Text Available PURPOSE: To investigate whether a third generation colloid, hydroxyethyl starch (HES 130/0.4, used for perioperative fluid therapy, protects the rat liver against the late-phase response of ischemia/reperfusion injury (IRI and if inhibition of neutrophil hepatic infiltration plays a part in this mechanism. METHODS: Wistar rats were used (8 in each group. Three groups had IRI induced by lobar vascular occlusion (60 minutes and reperfusion (24 hours and received HES (13 mL/kg iv, 7.5% saline (HS (13 mL/kg iv or no fluid. Three other groups were sham-operated and received the same fluid as the test groups. After 24 hours of reperfusion, blood was drawn for alanine aminotransferase (ALT quantification and ischemic liver samples were taken for histological study (hematoxylin and eosin and chloroacetate staining of neutrophils. RESULTS: HES treatment attenuated the elevation in serum ALT (P=0.001 and reduced the extent of hepatocellular necrosis (P0.05. CONCLUSION: Hydroxyethyl starch suppresses inflammatory response and ameliorates the late-phase response of hepatic ischemia/reperfusion injury.OBJETIVO: Investigar se um colóide de terceira geração (HES 130/0.4, utilizado para fluidoterapia perioperatória, protege o fígado de rato contra a resposta da fase tardia de isquemia/reperfusão e se a inibição da infiltração hepática de neutrófilos desempenha um papel neste mecanismo. MÉTODOS: Foram utilizados ratos Wistar (8 em cada grupo. Três grupos tiveram lesão de isquemia/reperfusão (IRI induzida por oclusão vascular lobar (60 minutos e reperfusão (24 horas e receberam HES (13 ml / kg iv, soro fisiológico a 7,5% (HS (13 ml / kg iv ou nenhum fluido. Três outros grupos foram sham-operados e receberam o mesmo tipo de fluido dos grupos de teste. Após 24 horas de reperfusão, o sangue foi retirado para quantificação da alanina aminotransferase (ALT e amostras de fígado isquêmico foram retiradas para estudo histológico (hematoxilina e

  4. Effect of fluid loading with normal saline and 6% hydroxyethyl starch on stroke volume variability and left ventricular volume

    Directory of Open Access Journals (Sweden)

    Kanda H

    2015-09-01

    Full Text Available Hirotsugu Kanda,1 Yuji Hirasaki,2 Takafumi Iida,1 Megumi Kanao,1 Yuki Toyama,1 Takayuki Kunisawa,1 Hiroshi Iwasaki,11Department of Anesthesiology and Critical Care Medicine, Asahikawa Medical University, Asahikawa, 2Department of Anatomy, The Jikei University Graduate School of Medicine, Tokyo, JapanPurpose: The aim of this clinical trial was to investigate changes in stroke volume variability (SVV and left ventricular end-diastolic volume (LVEDV after a fluid bolus of crystalloid or colloid using real-time three-dimensional transesophageal echocardiography (3D-TEE and the Vigileo-FloTrac™ system.Materials and methods: After obtaining Institutional Review Board approval, and informed consent from the research participants, 22 patients undergoing scheduled peripheral vascular bypass surgery were enrolled in the study. The patients were randomly assigned to receive 500 mL of hydroxyethyl starch (HES; HES group, n=11 or normal saline (Saline group, n=11 for fluid replacement therapy. SVV was measured using the Vigileo-FloTrac system. LVEDV, stroke volume, and cardiac output were measured by 3D-TEE. The measurements were performed over 30 minutes before and after the fluid bolus in both groups.Results: SVV significantly decreased after fluid bolus in both groups (HES group, 14.7%±2.6% to 6.9%±2.7%, P<0.001; Saline group, 14.3%±3.9% to 8.8%±3.1%, P<0.001. LVEDV significantly increased after fluid loading in the HES group (87.1±24.0 mL to 99.9±27.2 mL, P<0.001, whereas no significant change was detected in the Saline group (88.8±17.3 mL to 91.4±17.6 mL, P>0.05. Stroke volume significantly increased after infusion in the HES group (50.6±12.5 mL to 61.6±19.1 mL, P<0.01 but not in the Saline group (51.6±13.4 mL to 54.1±12.8 mL, P>0.05. Cardiac output measured by 3D-TEE significantly increased in the HES group (3.5±1.1 L/min to 3.9±1.3 L/min, P<0.05, whereas no significant change was seen in the Saline group (3.4±1.1 L/min to 3.3±1.0 L

  5. Quantification of energy savings from Ireland's Home Energy Saving scheme. An ex post billing analysis

    Energy Technology Data Exchange (ETDEWEB)

    Scheer, J.; Clancy, M.; Ni Hogain, S. [Sustainable Energy Authority of Ireland, Wilton Park House, Wilton Terrace, Dublin 2 (Ireland)

    2013-02-15

    This paper quantifies the energy savings realised by a sample of participants in the Sustainable Energy Authority of Ireland's Home Energy Saving (HES) residential retrofit scheme (currently branded as the Better Energy Homes scheme), through an ex post billing analysis. The billing data are used to evaluate: (1) the reduction in gas consumption of the sample between pre- (2008) and post- (2010) scheme participation when compared to the gas consumption of a control group, (2) an estimate of the shortfall when this result is compared to engineering-type ex ante savings estimates and (3) the degree to which these results may apply to the wider population. All dwellings in the study underwent energy efficiency improvements, including insulation upgrades (wall and/or roof), installation of high-efficiency boilers and/or improved heating controls, as part of the HES scheme. Metered gas use data for the 210 households were obtained from meter operators for a number of years preceding dwelling upgrades and for a post-intervention period of 1 year. Dwelling characteristics and some household behavioural data were obtained through a survey of the sample. The gas network operator provided anonymised data on gas usage for 640,000 customers collected over the same period as the HES sample. Dwelling type data provided with the population dataset enabled matching with the HES sample to increase the internal validity of the comparison between the control (matched population data) and the treatment (HES sample). Using a difference-in-difference methodology, the change in demand of the sample was compared with that of the matched population subset of gas-using customers in Ireland over the same time period. The mean reduction in gas demand as a result of energy efficiency upgrades for the HES sample is estimated as 21 % or 3,664{+-}603 kWh between 2008 and 2010. An ex ante estimate of average energy savings, based on engineering calculations (u value reductions and improved boiler

  6. Fucosylation Deficiency in Mice Leads to Colitis and Adenocarcinoma.

    Science.gov (United States)

    Wang, Yiwei; Huang, Dan; Chen, Kai-Yuan; Cui, Min; Wang, Weihuan; Huang, Xiaoran; Awadellah, Amad; Li, Qing; Friedman, Ann; Xin, William W; Di Martino, Luca; Cominelli, Fabio; Miron, Alex; Chan, Ricky; Fox, James G; Xu, Yan; Shen, Xiling; Kalady, Mathew F; Markowitz, Sanford; Maillard, Ivan; Lowe, John B; Xin, Wei; Zhou, Lan

    2017-01-01

    De novo synthesis of guanosine diphosphate (GDP)-fucose, a substrate for fucosylglycans, requires sequential reactions mediated by GDP-mannose 4,6-dehydratase (GMDS) and GDP-4-keto-6-deoxymannose 3,5-epimerase-4-reductase (FX or tissue specific transplantation antigen P35B [TSTA3]). GMDS deletions and mutations are found in 6%-13% of colorectal cancers; these mostly affect the ascending and transverse colon. We investigated whether a lack of fucosylation consequent to loss of GDP-fucose synthesis contributes to colon carcinogenesis. FX deficiency and GMDS deletion produce the same biochemical phenotype of GDP-fucose deficiency. We studied a mouse model of fucosylation deficiency (Fx-/- mice) and mice with the full-length Fx gene (controls). Mice were placed on standard chow or fucose-containing diet (equivalent to a control fucosylglycan phenotype). Colon tissues were collected and analyzed histologically or by enzyme-linked immunosorbent assays to measure cytokine levels; T cells also were collected and analyzed. Fecal samples were analyzed by 16s ribosomal RNA sequencing. Mucosal barrier function was measured by uptake of fluorescent dextran. We transplanted bone marrow cells from Fx-/- or control mice (Ly5.2) into irradiated 8-week-old Fx-/- or control mice (Ly5.1). We performed immunohistochemical analyses for expression of Notch and the hes family bHLH transcription factor (HES1) in colon tissues from mice and a panel of 60 human colorectal cancer specimens (27 left-sided, 33 right-sided). Fx-/- mice developed colitis and serrated-like lesions. The intestinal pathology of Fx-/- mice was reversed by addition of fucose to the diet, which restored fucosylation via a salvage pathway. In the absence of fucosylation, dysplasia appeared and progressed to adenocarcinoma in up to 40% of mice, affecting mainly the right colon and cecum. Notch was not activated in Fx-/- mice fed standard chow, leading to decreased expression of its target Hes1. Fucosylation deficiency

  7. Esimene võit sõjas Eesti teedel / Jüri Pihl

    Index Scriptorium Estoniae

    Pihl, Jüri, 1954-

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke, 6. aug. 2007, lk. 9; Harjumaa, 7. aug. 2007, lk. 2; Hiiu Leht, 7. aug. 2007, lk. 4; Lääne Elu, 7. aug. 2007, lk. 2; Järva Teataja, 7. aug. 2007, lk. 5; Nädaline, 7. aug. 2007, lk. 2; Sakala, 7. aug. 2007, lk. 2; Meie Maa, 7. aug. 2007, lk. 2; Võrumaa Teataja, 7. aug. 2007, lk. 2; Sõnumitooja, 7. aug. 2007, lk. 2; Vooremaa, 7. aug. 2007, lk. 2; Pärnu Postimees, 7. aug. 2007, lk. 15; Koit, 7. aug. 2007, lk. 6; Valgamaalane, 9. aug. 2007, lk. 2; Harju Ekspress, 10. aug. 2007, lk. 6; Virumaa Teataja, 14. aug. 2007, lk. 11. Surmaga lõppevate liiklusõnnetuste riskitegurite vähendamiseks on politsei võtnud suuna liiklusjärelevalve tõhustamisele, suunates liiklusjärelevalvesse 80 protsenti rohkem politseinikke. Siseminister nendib, et liiklusõnnetuste langustrendile on aidanud kaasa ka väärtushinnangute muutus ühiskonnas

  8. Kasvatusteaduslike tööde 2001. aasta riikliku konkursi võitjad on selgunud / Ulve Kala

    Index Scriptorium Estoniae

    Kala, Ulve, 1947-

    2001-01-01

    Konkursile esitatud töid hindas komisjon koosseisus TPÜ dots. U. Kala (esimees), HM-i üldhariduse osakonna peaekspert M. Kadakas, Eesti Akadeemilise Pedagoogika Seltsi esimees I. Kraav, TPÜ prof. J. Orn, TÜ dots. T. Pedastsaar, TPÜ dots. H. Sikka, HM-i teaduse talituse juhataja M. Toimet. Eesti keeles publitseeritud teadustöö preemia: E. Krull monograafia "Pedagoogilise psühholoogia käsiraamat" ja kogumikule "Kõnelev ja kõneldav inimene : Eesti erinevate eluvaldkondade diskursus", autorid V.-R. Ruus, V. Kalmus, H. Krull, T. Kuurme, M.-L. Laherand, M. Lauristin, M. Leino, A. Liimets, K. Loogma, R. Ruutsoo, E.-S. Sarv, J. Veimer, M. Volt. Võõrkeeles publitseeritud teadustöö preemia: J. Mikk monograafia "Textbook : Research and Writing", ja M. Veisson "Disabled Children - the Psychological Status of Parents and the Social Network of Siblings". Populaarteaduslike tööde preemia: kogumik "Õppejõudu otsimas", autorid L. Erilt, K. Mits, A. Kincaid, T. Kuurme, K. Kütt, K. Loogma, E. Lõhkivi, M. Lõhmus, J. Mikk, S. Priimägi, S. Ransdell, V.-R. Ruus, A. Viru, K. Vogelberg, R. Walker. Heino Liimetsa nimeline magistritöö preemia: E. Jakobson magistritöö "Liitklassid ja eriealiste laste koosõpetamine" (juhendaja dots. V. Maanso) ja S. Jehe magistritöö "Ülesanded õppija kujunemiseks : töövihik ja õpetajavihik eesti kirjanduse käsitlemiseks gümnaasiumis" (juhendaja dots. T. Pedastsaar)

  9. Recording of hospitalizations for acute exacerbations of COPD in UK electronic health care records

    Directory of Open Access Journals (Sweden)

    Rothnie KJ

    2016-11-01

    Full Text Available Kieran J Rothnie,1,2 Hana Müllerová,3 Sara L Thomas,2 Joht S Chandan,4 Liam Smeeth,2 John R Hurst,5 Kourtney Davis,3 Jennifer K Quint1,2 1Respiratory Epidemiology, Occupational Medicine and Public Health, National Heart and Lung Institute, Imperial College London, London, UK; 2Faculty of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, UK; 3Respiratory Epidemiology, GlaxoSmithKline R&D, Uxbridge, London; 4Medical School, 5UCL Respiratory, University College London, London, UK Background: Accurate identification of hospitalizations for acute exacerbations of chronic obstructive pulmonary disease (AECOPD within electronic health care records is important for research, public health, and to inform health care utilization and service provision. We aimed to develop a strategy to identify hospitalizations for AECOPD in secondary care data and to investigate the validity of strategies to identify hospitalizations for AECOPD in primary care data. Methods: We identified patients with chronic obstructive pulmonary disease (COPD in the Clinical Practice Research Datalink (CPRD with linked Hospital Episodes Statistics (HES data. We used discharge summaries for recent hospitalizations for AECOPD to develop a strategy to identify the recording of hospitalizations for AECOPD in HES. We then used the HES strategy as a reference standard to investigate the positive predictive value (PPV and sensitivity of strategies for identifying AECOPD using general practice CPRD data. We tested two strategies: 1 codes for hospitalization for AECOPD and 2 a code for AECOPD other than hospitalization on the same day as a code for hospitalization due to unspecified reason. Results: In total, 27,182 patients with COPD were included. Our strategy to identify hospitalizations for AECOPD in HES had a sensitivity of 87.5%. When compared with HES, using a code suggesting hospitalization for AECOPD in CPRD resulted in a PPV of 50.2% (95

  10. Identifying developmental toxicity pathways for a subset of ToxCast chemicals using human embryonic stem cells and metabolomics

    International Nuclear Information System (INIS)

    Kleinstreuer, N.C.; Smith, A.M.; West, P.R.; Conard, K.R.; Fontaine, B.R.; Weir-Hauptman, A.M.; Palmer, J.A.; Knudsen, T.B.; Dix, D.J.; Donley, E.L.R.; Cezar, G.G.

    2011-01-01

    Metabolomics analysis was performed on the supernatant of human embryonic stem (hES) cell cultures exposed to a blinded subset of 11 chemicals selected from the chemical library of EPA's ToxCast™ chemical screening and prioritization research project. Metabolites from hES cultures were evaluated for known and novel signatures that may be indicative of developmental toxicity. Significant fold changes in endogenous metabolites were detected for 83 putatively annotated mass features in response to the subset of ToxCast chemicals. The annotations were mapped to specific human metabolic pathways. This revealed strong effects on pathways for nicotinate and nicotinamide metabolism, pantothenate and CoA biosynthesis, glutathione metabolism, and arginine and proline metabolism pathways. Predictivity for adverse outcomes in mammalian prenatal developmental toxicity studies used ToxRefDB and other sources of information, including Stemina Biomarker Discovery's predictive DevTox® model trained on 23 pharmaceutical agents of known developmental toxicity and differing potency. The model initially predicted developmental toxicity from the blinded ToxCast compounds in concordance with animal data with 73% accuracy. Retraining the model with data from the unblinded test compounds at one concentration level increased the predictive accuracy for the remaining concentrations to 83%. These preliminary results on a 11-chemical subset of the ToxCast chemical library indicate that metabolomics analysis of the hES secretome provides information valuable for predictive modeling and mechanistic understanding of mammalian developmental toxicity. -- Highlights: ► We tested 11 environmental compounds in a hESC metabolomics platform. ► Significant changes in secreted small molecule metabolites were observed. ► Perturbed mass features map to pathways critical for normal development and pregnancy. ► Arginine, proline, nicotinate, nicotinamide and glutathione pathways were affected.

  11. Lunatic Fringe and p53 Cooperatively Suppress Mesenchymal Stem-Like Breast Cancer

    Directory of Open Access Journals (Sweden)

    Wen-Cheng Chung

    2017-11-01

    Full Text Available Claudin-low breast cancer (CLBC is a poor prognosis molecular subtype showing stemness and mesenchymal features. We previously discovered that deletion of a Notch signaling modulator, Lunatic Fringe (Lfng, in the mouse mammary gland induced a subset of tumors resembling CLBC. Here we report that deletion of one copy of p53 on this background not only accelerated mammary tumor development but also led to a complete penetrance of the mesenchymal stem-like phenotype. All mammary tumors examined in the Lfng/p53 compound mutant mice displayed a mesenchymal/spindloid pathology. These tumors showed high level expressions of epithelial-to-mesenchymal transition (EMT markers including Vimentin, Twist, and PDGFRα, a gene known to be enriched in CLBC. Prior to tumor onset, Lfng/p53 mutant mammary glands exhibited increased levels of Vimentin and E-cadherin, but decreased expressions of cytokeratin 14 and cytokeratin 8, accompanied by elevated basal cell proliferation and an expanded mammary stem cell-enriched population. Lfng/p53 mutant glands displayed increased accumulation of Notch3 intracellular fragment, up-regulation of Hes5 and down-regulation of Hes1. Analysis in human breast cancer datasets found the lowest HES1 and second lowest LFNG expressions in CLBC among molecular subtypes, and low level of LFNG is associated with poor survival. Immunostaining of human breast cancer tissue array found correlation between survival and LFNG immunoreactivity. Finally, patients carrying TP53 mutations express lower LFNG than patients with wild type TP53. Taken together, these data revealed genetic interaction between Lfng and p53 in mammary tumorigenesis, established a new mouse model resembling CLBC, and may suggest targeting strategy for this disease.

  12. Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.

    Directory of Open Access Journals (Sweden)

    Kyle J Hewitt

    2011-02-01

    Full Text Available Human induced pluripotent stem (hiPS cells offer a novel source of patient-specific cells for regenerative medicine. However, the biological potential of iPS-derived cells and their similarities to cells differentiated from human embryonic stem (hES cells remain unclear. We derived fibroblast-like cells from two hiPS cell lines and show that their phenotypic properties and patterns of DNA methylation were similar to that of mature fibroblasts and to fibroblasts derived from hES cells. iPS-derived fibroblasts (iPDK and their hES-derived counterparts (EDK showed similar cell morphology throughout differentiation, and patterns of gene expression and cell surface markers were characteristic of mature fibroblasts. Array-based methylation analysis was performed for EDK, iPDK and their parental hES and iPS cell lines, and hierarchical clustering revealed that EDK and iPDK had closely-related methylation profiles. DNA methylation analysis of promoter regions associated with extracellular matrix (ECM-production (COL1A1 by iPS- and hESC-derived fibroblasts and fibroblast lineage commitment (PDGFRβ, revealed promoter demethylation linked to their expression, and patterns of transcription and methylation of genes related to the functional properties of mature stromal cells were seen in both hiPS- and hES-derived fibroblasts. iPDK cells also showed functional properties analogous to those of hES-derived and mature fibroblasts, as seen by their capacity to direct the morphogenesis of engineered human skin equivalents. Characterization of the functional behavior of ES- and iPS-derived fibroblasts in engineered 3D tissues demonstrates the utility of this tissue platform to predict the capacity of iPS-derived cells before their therapeutic application.

  13. A review on recent size optimization methodologies for standalone solar and wind hybrid renewable energy system

    International Nuclear Information System (INIS)

    Al-falahi, Monaaf D.A.; Jayasinghe, S.D.G.; Enshaei, H.

    2017-01-01

    Highlights: • Possible combinations and configurations for standalone PV-WT HES were discussed. • Most recently used assessment parameters for standalone PV-WT HES were explained. • Optimization algorithms and software tools were comprehensively reviewed. • The recent trend of using hybrid algorithms over single algorithms was discussed. • Optimization algorithms for sizing standalone PV-WT HES were critically compared. - Abstract: Electricity demand in remote and island areas are generally supplied by diesel or other fossil fuel based generation systems. Nevertheless, due to the increasing cost and harmful emissions of fossil fuels there is a growing trend to use standalone hybrid renewable energy systems (HRESs). Due to the complementary characteristics, matured technologies and availability in most areas, hybrid systems with solar and wind energy have become the popular choice in such applications. However, the intermittency and high net present cost are the challenges associated with solar and wind energy systems. In this context, optimal sizing is a key factor to attain a reliable supply at a low cost through these standalone systems. Therefore, there has been a growing interest to develop algorithms for size optimization in standalone HRESs. The optimal sizing methodologies reported so far can be broadly categorized as classical algorithms, modern techniques and software tools. Modern techniques, based on single artificial intelligence (AI) algorithms, are becoming more popular than classical algorithms owing to their capabilities in solving complex optimization problems. Moreover, in recent years, there has been a clear trend to use hybrid algorithms over single algorithms mainly due to their ability to provide more promising optimization results. This paper aims to present a comprehensive review on recent developments in size optimization methodologies, as well as a critical comparison of single algorithms, hybrid algorithms, and software tools

  14. Advanced uncertainty modelling for container port risk analysis.

    Science.gov (United States)

    Alyami, Hani; Yang, Zaili; Riahi, Ramin; Bonsall, Stephen; Wang, Jin

    2016-08-13

    Globalization has led to a rapid increase of container movements in seaports. Risks in seaports need to be appropriately addressed to ensure economic wealth, operational efficiency, and personnel safety. As a result, the safety performance of a Container Terminal Operational System (CTOS) plays a growing role in improving the efficiency of international trade. This paper proposes a novel method to facilitate the application of Failure Mode and Effects Analysis (FMEA) in assessing the safety performance of CTOS. The new approach is developed through incorporating a Fuzzy Rule-Based Bayesian Network (FRBN) with Evidential Reasoning (ER) in a complementary manner. The former provides a realistic and flexible method to describe input failure information for risk estimates of individual hazardous events (HEs) at the bottom level of a risk analysis hierarchy. The latter is used to aggregate HEs safety estimates collectively, allowing dynamic risk-based decision support in CTOS from a systematic perspective. The novel feature of the proposed method, compared to those in traditional port risk analysis lies in a dynamic model capable of dealing with continually changing operational conditions in ports. More importantly, a new sensitivity analysis method is developed and carried out to rank the HEs by taking into account their specific risk estimations (locally) and their Risk Influence (RI) to a port's safety system (globally). Due to its generality, the new approach can be tailored for a wide range of applications in different safety and reliability engineering and management systems, particularly when real time risk ranking is required to measure, predict, and improve the associated system safety performance. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Identifying developmental toxicity pathways for a subset of ToxCast chemicals using human embryonic stem cells and metabolomics

    Energy Technology Data Exchange (ETDEWEB)

    Kleinstreuer, N.C., E-mail: kleinstreuer.nicole@epa.gov [NCCT, US EPA, RTP, NC 27711 (United States); Smith, A.M.; West, P.R.; Conard, K.R.; Fontaine, B.R. [Stemina Biomarker Discovery, Inc., Madison, WI 53719 (United States); Weir-Hauptman, A.M. [Covance, Inc., Madison, WI 53704 (United States); Palmer, J.A. [Stemina Biomarker Discovery, Inc., Madison, WI 53719 (United States); Knudsen, T.B.; Dix, D.J. [NCCT, US EPA, RTP, NC 27711 (United States); Donley, E.L.R. [Stemina Biomarker Discovery, Inc., Madison, WI 53719 (United States); Cezar, G.G. [Stemina Biomarker Discovery, Inc., Madison, WI 53719 (United States); University of Wisconsin-Madison, Madison, WI 53706 (United States)

    2011-11-15

    Metabolomics analysis was performed on the supernatant of human embryonic stem (hES) cell cultures exposed to a blinded subset of 11 chemicals selected from the chemical library of EPA's ToxCast Trade-Mark-Sign chemical screening and prioritization research project. Metabolites from hES cultures were evaluated for known and novel signatures that may be indicative of developmental toxicity. Significant fold changes in endogenous metabolites were detected for 83 putatively annotated mass features in response to the subset of ToxCast chemicals. The annotations were mapped to specific human metabolic pathways. This revealed strong effects on pathways for nicotinate and nicotinamide metabolism, pantothenate and CoA biosynthesis, glutathione metabolism, and arginine and proline metabolism pathways. Predictivity for adverse outcomes in mammalian prenatal developmental toxicity studies used ToxRefDB and other sources of information, including Stemina Biomarker Discovery's predictive DevTox Registered-Sign model trained on 23 pharmaceutical agents of known developmental toxicity and differing potency. The model initially predicted developmental toxicity from the blinded ToxCast compounds in concordance with animal data with 73% accuracy. Retraining the model with data from the unblinded test compounds at one concentration level increased the predictive accuracy for the remaining concentrations to 83%. These preliminary results on a 11-chemical subset of the ToxCast chemical library indicate that metabolomics analysis of the hES secretome provides information valuable for predictive modeling and mechanistic understanding of mammalian developmental toxicity. -- Highlights: Black-Right-Pointing-Pointer We tested 11 environmental compounds in a hESC metabolomics platform. Black-Right-Pointing-Pointer Significant changes in secreted small molecule metabolites were observed. Black-Right-Pointing-Pointer Perturbed mass features map to pathways critical for normal

  16. A feeder-free culture using autogeneic conditioned medium for undifferentiated growth of human embryonic stem cells: Comparative expression profiles of mRNAs, microRNAs and proteins among different feeders and conditioned media

    Directory of Open Access Journals (Sweden)

    Chou Chi-Hsien

    2010-10-01

    Full Text Available Abstract Background Human embryonic stem (hES cell lines were derived from the inner cell mass of human blastocysts, and were cultured on mouse embryonic fibroblast (MEF feeder to maintain undifferentiated growth, extensive renewal capacity, and pluripotency. The hES-T3 cell line with normal female karyotype was previously used to differentiate into autogeneic fibroblast-like cells (T3HDF as feeder to support the undifferentiated growth of hES-T3 cells (T3/HDF for 14 passages. Results A feeder-free culture on Matrigel in hES medium conditioned by the autogeneic feeder cells (T3HDF was established to maintain the undifferentiated growth of hES-T3 cells (T3/CMHDF for 8 passages in this investigation. The gene expression profiles of mRNAs, microRNAs and proteins between the undifferentiated T3/HDF and T3/CMHDF cells were shown to be very similar, and their expression profiles were also found to be similar to those of T3/MEF and T3/CMMEF cells grown on MEF feeder and feeder-free Matrigel in MEF-conditioned medium, respectively. The undifferentiated state of T3/HDF and T3/CMHDF as well as T3/MEF andT3/CMMEF cells was evidenced by the very high expression levels of "stemness" genes and low expression levels of differentiation markers of ectoderm, mesoderm and endoderm in addition to the strong staining of OCT4 and NANOG. Conclusion The T3HDF feeder and T3HDF-conditioned medium were able to support the undifferentiated growth of hES cells, and they would be useful for drug development and toxicity testing in addition to the reduced risks of xenogeneic pathogens when used for medical applications such as cell therapies.

  17. The changing face of urinary continence surgery in England: a perspective from the Hospital Episode Statistics database.

    Science.gov (United States)

    Withington, John; Hirji, Sadaf; Sahai, Arun

    2014-08-01

    To quantify changes in surgical practice in the treatment of stress urinary incontinence (SUI), urge urinary incontinence (UUI) and post-prostatectomy stress incontinence (PPI) in England, using the Hospital Episode Statistics (HES) database. We used public domain information from the HES database, an administrative dataset recording all hospital admissions and procedures in England, to find evidence of change in the use of various surgical procedures for urinary incontinence from 2000 to 2012. For the treatment of SUI, a general increase in the use of synthetic mid-urethral tapes, such as tension-free vaginal tape (TVTO) and transobturator tape (TOT), was observed, while there was a significant decrease in colposuspension procedures over the same period. The number of procedures to remove TVT and TOT has also increased in recent years. In the treatment of overactive bladder and UUI, there has been a significant increase in the use of botulinum toxin A and neuromodulation in recent years. This coincided with a steady decline in the recorded use of clam ileocystoplasty. A steady increase was observed in the insertion of artificial urinary sphincter (AUS) devices in men, related to PPI. Mid-urethral synthetic tapes now represent the mainstream treatment of SUI in women, but tape-related complications have led to an increase in procedures to remove these devices. The uptake of botulinum toxin A and sacral neuromodulation has led to fewer clam ileocystoplasty procedures being performed. The steady increase in insertions of AUSs in men is unsurprising and reflects the widespread uptake of radical prostatectomy in recent years. There are limitations to results sourced from the HES database, with potential inaccuracy of coding; however, these data support the trends observed by experts in this field. © 2014 The Authors. BJU International published by John Wiley & Sons Ltd on behalf of BJU International.

  18. Effect of different culture media and deswelling agents on survival of human corneal endothelial and epithelial cells in vitro.

    Science.gov (United States)

    Valtink, Monika; Donath, Patricia; Engelmann, Katrin; Knels, Lilla

    2016-02-01

    To examine the effects of media and deswelling agents on human corneal endothelial and epithelial cell viability using a previously developed screening system. The human corneal endothelial cell line HCEC-12 and the human corneal epithelial cell line HCE-T were cultured in four different corneal organ culture media (serum-supplemented: MEM +2 % FCS, CorneaMax®/CorneaJet®, serum-free: Human Endothelial-SFM, Stemalpha-2 and -3) with and without 6 % dextran T500 or 7 % HES 130/0.4. Standard growth media F99HCEC and DMEM/F12HCE-T served as controls. In additional controls, the stress inducers staurosporine or hydrogen peroxide were added. After 5 days in the test media, cell viability was assessed by flow cytometrically quantifying apoptotic and necrotic cells (sub-G1 DNA content, vital staining with YO-PRO-1® and propidium iodide) and intracellular reactive oxygen species (ROS). The MEM-based media were unable to support HCEC-12 and HCE-T survival under stress conditions, resulting in significantly increased numbers of apoptotic and necrotic cells. HCEC-12 survival was markedly improved in SFM-based media even under staurosporine or hydrogen peroxide. Likewise, HCE-T survival was improved in SFM with or without dextran. The media CorneaMax®, CorneaJet®, and CorneaMax® with HES supported HCEC-12 survival better than MEM-based media, but less well than SFM-based media. HCE-T viability was also supported by CorneaJet®, but not by CorneaMax® with or without HES. Stemalpha-based media were not suitable for maintaining viability of HCEC-12 or HCE-T in the applied cell culture system. The use of serum-supplemented MEM-based media for corneal organ culture should be discontinued in favour of serum-free media like SFM.

  19. Time to achieve target mean arterial pressure during resuscitation from experimental anaphylactic shock in an animal model. A comparison of adrenaline alone or in combination with different volume expanders.

    Science.gov (United States)

    Tajima, K; Zheng, F; Collange, O; Barthel, G; Thornton, S N; Longrois, D; Levy, B; Audibert, G; Malinovsky, J M; Mertes, P M

    2013-11-01

    Anaphylactic shock is a rare, but potentially lethal complication, combining life-threatening circulatory failure and massive fluid shifts. Treatment guidelines rely on adrenaline and volume expansion by intravenous fluids, but there is no solid evidence for the choice of one specific type of fluid over another. Our purpose was to compare the time to achieve target mean arterial pressure upon resuscitation using adrenaline alone versus adrenaline with different resuscitation fluids in an animal model and to compare the tissue oxygen pressures (PtiO2) with the various strategies. Twenty-five ovalbumin-sensitised Brown Norway rats were allocated to five groups after anaphylactic shock induction: vehicle (CON), adrenaline alone (AD), or adrenaline with isotonic saline (AD+IS), hydroxyethyl starch (AD+HES) or hypertonic saline (AD+HS). Time to reach a target mean arterial pressure value of 75 mmHg, cardiac output, skeletal muscle PtiO2, lactate/pyruvate ratio and cumulative doses of adrenaline were recorded. Non-treated rats died within 15 minutes. The target mean arterial pressure value was reached faster with AD+HES (median: 10 minutes, range: 7.5 to 12.5 minutes) and AD+IS (median: 17.5 minutes, range: 5 to 25 minutes) versus adrenaline alone (median: 25 minutes, range: 20-30 minutes). There were also reduced adrenaline requirements in these groups. The skeletal muscle PtiO2 was restored only in the AD+HES group. Although direct extrapolation to humans should be made with caution, our results support the combined use of adrenaline and volume expansion for resuscitation from anaphylactic shock. When used with adrenaline the most effective fluid was hydroxyethyl starch, whereas hypertonic saline was the least effective.

  20. Anfangen, sich die Welt anders vorzustellen

    Directory of Open Access Journals (Sweden)

    Sabine Fuchs

    2006-11-01

    Full Text Available Der Sammelband Butler Matters bezeugt Judith Butlers außerordentliche Bedeutung für feministische und queere Studien und Forschung. In elf Beiträgen wird Butlers (frühes Werk von Gender Trouble und Bodies That Matter über Excitable Speech bis zu The Psychic Life of Power betrachtet und sein weit reichender Einfluss quer durch die wissenschaftlichen Disziplinen nachgewiesen. Dargestellt werden auch die kontroverse Rezeption von Butlers Werk sowie die politischen Wirkungen von Butlers Denken. Besonders anregend ist ein zweiteiliges Interview mit Judith Butler, in dem sie auf ein breites Spektrum von Fragen eingeht, die auch wichtige Themen ihrer aktuelleren Werke betreffen.

  1. Coordinated Research Program in Pulsed Power Physics.

    Science.gov (United States)

    1987-02-16

    II 1 Associate Investigator and 11 Graduate Students. Other faculty investigators from Electrical Engineerings, Physics and Chemistry , also...admixtu4 P’.*rs’ OfC0 o as atar 12. i. 4 hws 11.BUNAY FECSAN ... AILTE SCHAEFER AND SCHOENRACH: DIFFUSE DISCHARGE oPENiNG swrrcHEs - 40 increasing velocity...a complete set of cross sections is available for N2 18) and the plasma . chemistry in a mixture of N2 and N20 appeared to be U a s is 2 2 relatively

  2. Markkinointisuunnitelma - Internet startup

    OpenAIRE

    Koivu, Sami; Kantonen, Erno

    2011-01-01

    Tämän toiminnallisen opinnäytetyön aiheena oli luoda markkinointisuunnitelma kehitteillä olevalle Internet-palvelulle, joka on digitaalisesti toimiva yhteisöllinen musiikkipalvelu. Palvelun mahdolliset liiketoimintamahdollisuudet perustuvat lähes täysin suureen käyttäjämäärään. Siksi työn tarkoituksena oli selvittää, miten palvelulle saavutetaan mahdollisimman paljon käyttäjiä, ja miten nämä käyttäjät aktivoidaan tuottamaan lisäarvoa palvelulle. Työn lopullisena tavoitteena oli markkinointisu...

  3. Notch-mediated post-translational control of Ngn3 protein stability regulates pancreatic patterning and cell fate commitment

    DEFF Research Database (Denmark)

    Qu, Xiaoling; Afelik, Solomon; Jensen, Jan Nygaard

    2013-01-01

    of ducts. On one hand, Ngn3 cell-intrinsically activates endocrine target genes; on the other, Ngn3 cell-extrinsically promotes lateral signaling via the Dll1>Notch>Hes1 pathway which substantially limits its ability to sustain endocrine formation. Prior to endocrine commitment, the Ngn3-mediated...... involves transcriptional repression as previously shown, but also incorporates a novel post-translational mechanism. In addition to its ability to promote endocrine fate, we provide evidence of a competing ability of Ngn3 in the patterning of multipotent progenitor cells in turn controlling the formation...

  4. Βιβλιοκρισία: ΑNTJE BOSSELMANN – RUICKBIE, Byzantinischer Schmuck des 9. bis frühen 13. Jahrhunderts, Reichert Verlag, Wiesbaden 2011.

    Directory of Open Access Journals (Sweden)

    Tζένη ΑΛΜΠΑΝΗ

    2013-02-01

    Full Text Available Biιβλιοκρισία του: Αntje Bosselmann – Ruickbie, Byzantinischer Schmuck des 9. bis frühen 13. Jahrhunderts. Untersuchungen zum metallen dekorativen Körperschmuck der mittelbyzantinische Zeit anhand datierter Funde [Spätantike – Frühes Christentum – Byzanz. Kunst im ersten Jahrtausend. Hsgb. von Beat Brenk, Johannes G. Deckers, Arne Effenberger, Franz Alto Bauer. Reihe B: Studien und Perspektiven. Bd. 28], Reichert Verlag, Wiesbaden 2011, 420 σσ. +832 εικ.([ISBN 978-3-89500-717-0 

  5. Status Report on the High-Temperature Steam Electrolysis Plant Model Developed in the Modelica Framework (FY17)

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jong Suk [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bragg-Sitton, Shannon M. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Boardman, Richard D. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2017-08-29

    This report has been prepared as part of an effort to design and build a modeling and simulation (M&S) framework to assess the economic viability of a nuclear-renewable hybrid energy system (N-R HES). In order to facilitate dynamic M&S of such an integrated system, research groups in multiple national laboratories have been developing various subsystems as dynamic physics-based components using the Modelica programming language. In fiscal year 2015 (FY15), Idaho National Laboratory (INL) performed a dynamic analysis of two region-specific N-R HES configurations, including the gas-to-liquid (natural gas to Fischer-Tropsch synthetic fuel) and brackish water reverse osmosis desalination plants as industrial processes. In FY16, INL developed two additional subsystems in the Modelica framework: (1) a high-temperature steam electrolysis (HTSE) plant as a high priority industrial plant to be integrated with a light water reactor (LWR) within an N-R HES and (2) a gas turbine power plant as a secondary energy supply. In FY17, five new components (i.e., a feedwater pump, a multi-stage compression system, a sweep-gas turbine, flow control valves, and pressure control valves) have been incorporated into the HTSE system proposed in FY16, aiming to better realistically characterize all key components of concern. Special attention has been given to the controller settings based on process models (i.e., direct synthesis method), aiming to improve process dynamics and controllability. A dynamic performance analysis of the improved LWR/HTSE integration case was carried out to evaluate the technical feasibility (load-following capability) and safety of such a system operating under highly variable conditions requiring flexible output. The analysis (evaluated in terms of the step response) clearly shows that the FY17 model resulted in superior output responses with much smaller settling times and less oscillatory behavior in response to disturbances in the electric load than those

  6. Feasibility Report. Mississippi River at Saint Paul, Minnesota. Reevaluation of Saint Paul Flood Control Project.

    Science.gov (United States)

    1981-09-01

    formal consultation process should be initiated. This should be done by writing to the Regional Director, U. S. Fish and Wildlife Service, Federal...16 5 FC.F PIPFRiF G;~IPHFiTIOtl LAYEP THIi>.NE*:7. 12. Ilit HES’ PERCENT LI’THTEP LIfIIT:- OFTfE B:Y WJEIGHT (JE,: I GHT LI. 15 12 wRITE FUAJLT- OIF...Paul to 8 inches in the headwaters. Cold weather nearly halted snow ielt runoff during the latter part of March, allowing many streams to drop below

  7. Palveluprosessin kuvaus lääkärivastaanotolla Service Blueprintin avulla

    OpenAIRE

    Nurminen, Jenni

    2016-01-01

    Palveluprosessi on erilaisten toimintojen sarja, jonka tuotoksena asiakas saa hyödyn. Asiakas ei näe palvelu prosessina, vaan keinona saavuttaa omat tavoitteensa. Hän näkee vain palveluprosessin vuorovaikutteisen osan ja tukiosa on lähes näkymätön asiakkaalle. Jokainen asiakas kokee palvelun eri tavalla ja muodostaa yksilöllisen asiakaskokemuksen palvelusta. Palveluliiketoiminnassa on tärkeää yrittää suunnitella prosessi vastaamaan mahdollisimman hyvin asiakkaan tarpeisiin ja toiveisiin. Asia...

  8. "Joo mä lopetan pian!" : Katsaus nuorten pelitottumuksiin ja pelien vaikutuksiin

    OpenAIRE

    Heikkuri, Matti; Vuorenmaa, Jan-Markus

    2013-01-01

    Videopelaaminen on yleistynyt yhä enemmän viime vuosien aikana ja lähes jokainen nuori pelaa niitä. Pelaamisesta on tullut myös paljon globaalimpi ilmiö verkkopelimahdollisuuksien lisääntymisen seurauksena. Nykyisin videopeleistä puhutaan mediassa lähinnä negatiivisena ilmiönä. Videopelaamisella on kuitenkin sekä positiivisia, että negatiivisia vaikutuksia, joista tulisi olla tietoinen. Tavoitteenamme oli tuottaa hyvä yleistietopaketti nuorten pelitottumuksista ja pelien vaikutuksista, jo...

  9. Flurbiprofen and hypertension but not hydroxyethyl starch are associated with post-craniotomy intracranial haematoma requiring surgery.

    Science.gov (United States)

    Jian, M; Li, X; Wang, A; Zhang, L; Han, R; Gelb, A W

    2014-11-01

    Post-craniotomy intracranial haematoma is one of the most serious complications after neurosurgery. We examined whether post-craniotomy intracranial haematoma requiring surgery is associated with the non-steroidal anti-inflammatory drugs flurbiprofen, hypertension, or hydroxyethyl starch (HES). A case-control study was conducted among 42 359 patients who underwent elective craniotomy procedures at Beijing Tiantan Hospital between January 2006 and December 2011. A one-to-one control group without post-craniotomy intracranial haematoma was selected matched by age, pathologic diagnosis, tumour location, and surgeon. Perioperative blood pressure records up to the diagnosis of haematoma, the use of flurbiprofen and HES were examined. The incidence of post-craniotomy intracranial haematoma and the odds ratios for the risk factors were determined. A total of 202 patients suffered post-craniotomy intracranial haematoma during the study period, for an incidence of 0.48% (95% CI=0.41-0.55). Haematoma requiring surgery was associated with an intraoperative systolic blood pressure of >160 mm Hg (OR=2.618, 95% CI=2.084-2.723, P=0.007), an intraoperative mean blood pressure of >110 mm Hg (OR=2.600, 95% CI=2.312-3.098, P=0.037), a postoperative systolic blood pressure of >160 mm Hg (OR=2.060, 95% CI= 1.763-2.642, P=0.022), a postoperative mean blood pressure of >110 mm Hg (OR=3.600, 95% CI= 3.226-4.057, P=0.001), and the use of flurbiprofen during but not after the surgery (OR=2.256, 95% CI=2.004-2.598, P=0.005). The intraoperative infusion of HES showed no significant difference between patients who had a haematoma and those who did not. Intraoperative and postoperative hypertension and the use of flurbiprofen during surgery are risk factors for post-craniotomy intracranial haematoma requiring surgery. The intraoperative infusion of HES was not associated with a higher incidence of haematoma. © The Author 2014. Published by Oxford University Press on behalf of the British

  10. A comparison of healthcare resource use for rotavirus and RSV between vulnerable children with co-morbidities and healthy children: a case control study.

    Science.gov (United States)

    Pockett, Rhys D; Campbell, David; Carroll, Stuart; Rajoriya, Fiona; Adlard, Nick

    2013-01-01

    To quantify the differences in hospital length of stay (LOS) and cost between healthy and vulnerable children with cystic fibrosis (CF), insulin-dependent diabetes mellitus (IDDM), cancer, and epilepsy who contract rotavirus (RVGE) or respiratory syncytial virus (RSV). Hospital Episode Statistics (HES) data were collected for England, for children consideration, as for rotavirus vaccines, evaluation of a vaccination programme should consider the potentially positive impact on vulnerable children. Limitations of the study include a dependency on accurate coding, an expectation that patients are identified through laboratory testing, and the possibility of unidentified underlying conditions affecting the burden.

  11. The impact of glaucoma referral refinement criteria on referral to, and first-visit discharge rates from, the hospital eye service: the Health Innovation & Education Cluster (HIEC) Glaucoma Pathways project.

    Science.gov (United States)

    Ratnarajan, Gokulan; Newsom, Wendy; French, Karen; Kean, Jane; Chang, Lydia; Parker, Mike; Garway-Heath, David F; Bourne, Rupert R A

    2013-03-01

    To assess the impact of referral refinement criteria on the number of patients referred to, and first-visit discharges from, the Hospital Eye Service (HES) in relation to the National Institute for Health & Clinical Excellence (NICE) Glaucoma Guidelines, Joint College Group Guidance (JCG) and the NICE commissioning guidance. All low-risk (one risk factor: suspicious optic disc, abnormal visual field (VF), raised intra-ocular pressure (IOP) (22-28 mmHg) or IOP asymmetry (>5 mmHg) and high-risk (more than one risk factor, shallow anterior chamber or IOP >28 mmHg) referrals to the HES from 2006 to 2011 were analysed. Low-risk referrals were seen by Optometrists with a specialist interest in glaucoma and high-risk referrals were referred directly to the HES. Two thousand nine hundred and twelve patient records were analysed. The highest Consultant first-visit discharge rates were for referrals based on IOP alone (45% for IOP 22-28 mmHg) and IOP asymmetry (53%), VF defect alone (46%) and for abnormal IOP and VF (54%). The lowest first-visit discharge rates were for referrals for suspicious optic disc (19%) and IOP >28 mmHg (22%). 73% of patients aged 65-80 and 60% of patients aged >80 who were referred by the OSI due to an IOP between 22-28 mmHg would have satisfied the JCG criteria for non-referral. For patients referred with an IOP >28 mmHg and an otherwise normal examination, adherence to the NICE commissioning guidance would have resulted in 6% fewer referrals. In 2010 this scheme reduced the number of patients attending the HES by 15%, which resulted in a saving of £16 258 (13%). The results support that referrals for a raised IOP alone or in combination with an abnormal VF be classified as low-risk and undergo referral refinement. Adherence to the JCG and the NICE commissioning guidance as onward referral criteria for specialist optometrists in this referral refinement scheme would result in fewer referrals. Ophthalmic & Physiological Optics © 2013 The College

  12. Haldusreform - mis ja milleks / Kalle Jürgenson

    Index Scriptorium Estoniae

    Jürgenson, Kalle, 1960-

    2001-01-01

    Riigi haldamise kolme tasandit - riigi keskvõimu, maakonna ja kohalike omavalitsuste tasandit - on vaja ühes või teises osas reformida. Autor: Isamaaliit. Parlamendisaadik. Ilmunud ka: Võrumaa Teataja, 10. apr. 2001, lk. 2; Põhjarannik, 10. apr. 2001, lk. 2; Severnoje Poberezhje, 10. apr. 2001, lk. 2; Meie Maa, 10. apr. 2001, lk. 2; Lääne Elu, 10. apr. 2001, lk. 4; Vooremaa, 10. apr. 2001, lk. 2; Nädaline, 10. apr. 2001, lk. 5; Virumaa Teataja, 18. apr. 2001, lk. 7; Infoleht : Isamaaliit, apr., nr. 27, 2001, lk. 8

  13. Skeittielokuva : eilisestä huomiseen

    OpenAIRE

    Ruotsalainen, Perttu

    2010-01-01

    Rullalauta- eli skeittielokuvia on tehty lähes yhtä pitkään kuin laji on ollut olemassa. Teknologian kehittymisen myötä skeittielokuvien tekeminen on vakiinnuttanut jalansijaa harrastajien parissa, kun se ennen oli puhtaasti skeittifirmojen markkinointiväline. Toiminnallisen opinnäytetyön kirjallisessa osassa käydään läpi lyhyesti rullalautailun historiaa aina lajin syntypäiviltä nykyaikaan. Jotta skeittikulttuurista ja siihen liittyvistä ilmiöistä, kuten skeittivideoista voisi jotain ymm...

  14. Kuorma-auton päällirakenteen valmistus

    OpenAIRE

    Murtonen, Juha

    2010-01-01

    Kuorma-autot ovat hyötyajoneuvoja, ja lähes poikkeuksetta jokaiseen kuorma-autoon kuuluu päällirakenne. Tämän opinnäytetyön tarkoituksena on selvittää ja dokumentoida MAN TGM 13.240 4x4 –kuorma-auton päällirakenteen valmistusvaiheet. Työn toimeksiantajana on mikkeliläislähtöinen raskaankaluston korjaamo Mikkelin Rekkakorjaamo Ky. Kyseinen yritys toimii Mikkelissä on MAN, Iveco ja DAF –merkkihuoltamona. Tarkoituksena on piirtää selkeälukuiset tekniset mittapiirustukset päällirakenteesta ko...

  15. Ajatuksista tekoihin : koetut attribuutiot ja muut tekijät itsemurha-ajatusten taustalla

    OpenAIRE

    Kippo, Saara

    2013-01-01

    hes sata ihmistä surmaa itsensä joka tunti ympäri maailmaa. Huomattavasti suurempi määrä yksilöitä ajattelee itsemurhaa juuri nyt. Kehittyneimmissä maissa erilaiset projektit ja eettiset valtakunnalliset tavoitteet pyrkivät vähentämään oman maansa itsemurhalukemia selvittämällä millaisilla palveluilla ja muutoksilla itsemurhia voidaan ehkäistä. Yksi suuri teema aihetta pohtiessa on se liittyykö itsemurhaan ja itsemurha-ajatuksiin aina mielenterveydellinen häiriö, vai voivatko itsemurha-ajat...

  16. ‚esponse of —n o™e—n gener—l ™ir™ul—tion model to wind —nd ...

    Indian Academy of Sciences (India)

    exe™utionF ƒome of these —dv—nt—ges m—ke this. —ppro—™h prefer—˜le over other —ppro—™hesF. „he wind field used for these simul—tion experiE ments —re derived from igw‡p ews€ —r™hivesF „he experiment w—s performed ˜y spinning up the model from — st—te of rest with pres™ri˜ed thermodyn—mi™.

  17. Tallinna Linnateatri 5. rahvusvaheline teatrifestival "Talveöö unenägu" 28.-30. detsembril 2008 / Laur Kaunissaare

    Index Scriptorium Estoniae

    Kaunissaare, Laur, 1982-

    2008-01-01

    Festivalilavastustest - Fjodor Dostojevski "Vendade Karamazovite" järgi "Suurinkvisiitor" (Théatre des Bouffes du Nord, lavastaja Peter Brook), Tom Peuckerti "Artaud meenutab Adolf Hitlerit ja Romanische Café'd" (Berliner Ensemble, lavastaja Paul Plamper), Tatjana Tolstaja "Sonja" (Riia Uus Teater, lavastaja Alvis Hermanis), Lot Vekemansi "Tema õde" (NT Gent, lavastaja Allan Zipson), Jan Jönsoni "Tõelushetked" (autor, lavastaja ja esitaja ühes isikus) ja festivalil esinevatest erakordsetest näitlejatest Peter Wuttkest, Bruce Myersist, Elsie de Brauwist, Jan Jönsonist

  18. RED ONE kuvaajan työkaluna

    OpenAIRE

    Käki, Ossi

    2010-01-01

    Opinnäytetyöni projektiosa, 30-minuuttinen lyhytelokuva Huomisen Muisto – Memory of Tomorrow kuvattiin RED ONE -kameralla vuosina 2008-2009. Opinnäytetyöni kirjallinen osuus tutkii miten RED ONE soveltuu kuvaajan työvälineeksi lyhytelokuvassa. Huomisen Muisto oli ensimmäinen Tampereen ammattikorkeakoulussa RED ONE:lla kuvattu elokuva ja koko tekninen workflow piti kehittää lähes nollasta. Paljon kuvan digitaalista jälkikäsittelyä sisältävänä science fiction -elokuvana Huomisen Muisto asetti i...

  19. Eosinophilic granulomatosis with polyangiitis (formerly known as Churg-Strauss syndrome as a differential diagnosis of hypereosinophilic syndromes

    Directory of Open Access Journals (Sweden)

    Yuri Albuquerque Pessoa Santos

    2017-01-01

    Full Text Available Eosinophilic granulomatosis with polyangiitis (EGPA, formerly known as Churg-Strauss syndrome, is a rare systemic disease situated between primary small vessel vasculitides associated with antineutrophil cytoplasmic antibodies (ANCAs and hypereosinophilic syndromes (HES. Here, we present a case of EGPA in a 38-year-old male, with a previous diagnosis of asthma, who presented with fever, migratory lung infiltrates and systemic eosinophilia that was refractory to previous courses of antibiotics. This case highlights the importance of the primary care physician understanding the differential diagnosis of pulmonary eosinophilic syndromes.

  20. Henkilöstövoimavarojen johtamista megamarketissa

    OpenAIRE

    Setälä, Minna

    2016-01-01

    Henkilöstö on lähes kaikkien organisaatioiden tärkein voimavara ja menestyksen perusta. Ilman henkilöstön olemassaoloa ja osaamista, ei mikään organisaatio pystyisi toimimaan. Henkilöstövoimavarojen johtamisen tulisikin olla keskeinen osa yrityksen strategista toimintaa. Osaava ja hyvinvoiva henkilöstö tuottaa luonnollisesti vastinetta siihen sijoitetulle pääomalle. Tämä opinnäytetyö tehtiin Vantaalla sijaitsevalle elintarvike- ja päivittäistavaraketjun myymälälle, supermarketille. Tavo...