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Sample records for pol iii holoenzyme

  1. The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.

    Science.gov (United States)

    Burgers, P M; Kornberg, A; Sakakibara, Y

    1981-09-01

    An Escherichia coli mutant, dnaN59, stops DNA synthesis promptly upon a shift to a high temperature; the wild-type dnaN gene carried in a transducing phage encodes a polypeptide of about 41,000 daltons [Sakakibara, Y. & Mizukami, T. (1980) Mol. Gen. Genet. 178, 541-553; Yuasa, S. & Sakakibara, Y. (1980) Mol. Gen. Genet. 180, 267-273]. We now find that the product of dnaN gene is the beta subunit of DNA polymerase III holoenzyme, the principal DNA synthetic multipolypeptide complex in E. coli. The conclusion is based on the following observations: (i) Extracts from dnaN59 cells were defective in phage phi X174 and G4 DNA synthesis after the mutant cells had been exposed to the increased temperature. (ii) The enzymatic defect was overcome by addition of purified beta subunit but not by other subunits of DNA polymerase III holoenzyme or by other replication proteins required for phi X174 DNA synthesis. (iii) Partially purified beta subunit from the dnaN mutant, unlike that from the wild type, was inactive in reconstituting the holoenzyme when mixed with the other purified subunits. (iv) Increased dosage of the dnaN gene provided by a plasmid carrying the gene raised cellular levels of the beta subunit 5- to 6-fold.

  2. Dynamics of termination during in vitro replication of ultraviolet-irradiated DNA with DNA polymerase III holoenzyme of Escherichia coli

    International Nuclear Information System (INIS)

    Shwartz, H.; Livneh, Z.

    1987-01-01

    During in vitro replication of UV-irradiated single-stranded DNA with Escherichia coli DNA polymerase III holoenzyme termination frequently occurs at pyrimidine photodimers. The termination stage is dynamic and characterized by at least three different events: repeated dissociation-reinitiation cycles of the polymerase at the blocked termini; extensive hydrolysis of ATP to ADP and inorganic phosphate; turnover of dNTPs into dNMP. The reinitiation events are nonproductive and are not followed by further elongation. The turnover of dNTPs into dNMPs is likely to result from repeated cycles of insertion of dNMP residues opposite the blocking lesions followed by their excision by the 3'----5' exonucleolytic activity of the polymerase. Although all dNTPs are turned over, there is a preference for dATP, indicating that DNA polymerase III holoenzyme has a preference for inserting a dAMP residue opposite blocking pyrimidine photodimers. We suggest that the inability of the polymerase to bypass photodimers during termination is due to the formation of defective initiation-like complexes with reduced stability at the blocked termini

  3. Replication of UV-irradiated single-stranded DNA by DNA polymerase III holoenzyme of Escherichia coli: evidence for bypass of pyrimidine photodimers

    International Nuclear Information System (INIS)

    Livneh, Z.

    1986-01-01

    Replication of UV-irradiated circular single-stranded phage M13 DNA by Escherichia coli RNA polymerase (EC 2.7.7.6) and DNA polymerase III holoenzyme (EC 2.7.7.7) in the presence of single-stranded DNA binding protein yielded full-length as well as partially replicated products. A similar result was obtained with phage G4 DNA primed with E. coli DNA primase, and phage phi X174 DNA primed with a synthetic oligonucleotide. The fraction of full-length DNA was several orders of magnitude higher than predicted if pyrimidine photodimers were to constitute absolute blocks to DNA replication. Recent models have suggested that pyrimidine photodimers are absolute blocks to DNA replication and that SOS-induced proteins are required to allow their bypass. Our results demonstrate that, under in vitro replication conditions, E. coli DNA polymerase III holoenzyme can insert nucleotides opposite pyrimidine dimers to a significant extent, even in the absence of SOS-induced proteins

  4. Only one ATP-binding DnaX subunit is required for initiation complex formation by the Escherichia coli DNA polymerase III holoenzyme.

    Science.gov (United States)

    Wieczorek, Anna; Downey, Christopher D; Dallmann, H Garry; McHenry, Charles S

    2010-09-17

    The DnaX complex (DnaX(3)δδ'χ psi) within the Escherichia coli DNA polymerase III holoenzyme serves to load the dimeric sliding clamp processivity factor, β(2), onto DNA. The complex contains three DnaX subunits, which occur in two forms: τ and the shorter γ, produced by translational frameshifting. Ten forms of E. coli DnaX complex containing all possible combinations of wild-type or a Walker A motif K51E variant τ or γ have been reconstituted and rigorously purified. DnaX complexes containing three DnaX K51E subunits do not bind ATP. Comparison of their ability to support formation of initiation complexes, as measured by processive replication by the DNA polymerase III holoenzyme, indicates a minimal requirement for one ATP-binding DnaX subunit. DnaX complexes containing two mutant DnaX subunits support DNA synthesis at about two-thirds the level of their wild-type counterparts. β(2) binding (determined functionally) is diminished 12-30-fold for DnaX complexes containing two K51E subunits, suggesting that multiple ATPs must be bound to place the DnaX complex into a conformation with maximal affinity for β(2). DNA synthesis activity can be restored by increased concentrations of β(2). In contrast, severe defects in ATP hydrolysis are observed upon introduction of a single K51E DnaX subunit. Thus, ATP binding, hydrolysis, and the ability to form initiation complexes are not tightly coupled. These results suggest that although ATP hydrolysis likely enhances β(2) loading, it is not absolutely required in a mechanistic sense for formation of functional initiation complexes.

  5. Mechanism of replication of ultraviolet-irradiated single-stranded DNA by DNA polymerase III holoenzyme of Escherichia coli. Implications for SOS mutagenesis

    International Nuclear Information System (INIS)

    Livneh, Z.

    1986-01-01

    Replication of UV-irradiated oligodeoxynucleotide-primed single-stranded phi X174 DNA with Escherichia coli DNA polymerase III holoenzyme in the presence of single-stranded DNA-binding protein was investigated. The extent of initiation of replication on the primed single-stranded DNA was not altered by the presence of UV-induced lesions in the DNA. The elongation step exhibited similar kinetics when either unirradiated or UV-irradiated templates were used. Inhibition of the 3'----5' proofreading exonucleolytic activity of the polymerase by dGMP or by a mutD mutation did not increase bypass of pyrimidine photodimers, and neither did purified RecA protein influence the extent of photodimer bypass as judged by the fraction of full length DNA synthesized. Single-stranded DNA-binding protein stimulated bypass since in its absence the fraction of full length DNA decreased 5-fold. Termination of replication at putative pyrimidine dimers involved dissociation of the polymerase from the DNA, which could then reinitiate replication at other available primer templates. Based on these observations a model for SOS-induced UV mutagenesis is proposed

  6. The beta subunit modulates bypass and termination at UV lesions during in vitro replication with DNA polymerase III holoenzyme of Escherichia coli

    International Nuclear Information System (INIS)

    Shavitt, O.; Livneh, Z.

    1989-01-01

    The cycling time of DNA polymerase III holoenzyme during replication of UV-irradiated single-stranded (ss) DNA was longer than with unirradiated DNA (8 versus 3 min, respectively), most likely due to slow dissociation from lesion-terminated nascent DNA strands. Initiation of elongation on primed ssDNA was not significantly inhibited by the presence of UV lesions as indicated by the identical distribution of replication products synthesized at early and late reaction times and by the identical duration of the initial synthesis bursts on both unirradiated and UV-irradiated DNA templates. When replication was performed with DNA polymerase III* supplemented with increasing quantities of purified beta 2 subunit, the cycling time on UV-irradiated DNA decreased from 14.8 min at 1.7 nM beta 2 down to 6 min at 170 nM beta 2, a concentration in which beta 2 was in large excess over the polymerase. In parallel to the reduction in cycling time, also the bypass frequency of cyclobutane-photodimers decreased with increasing beta 2 concentration, and at 170 nM beta 2, bypass of photodimers was essentially eliminated. It has been shown that polymerase complexes with more than one beta 2 per polymerase molecule were formed at high beta 2 concentrations. It is plausible that polymerase complexes obtained under high beta 2 concentration dissociate from lesion-terminated primers faster than polymerase complexes formed at a low beta 2 concentration. This is expected to favor termination over bypass at pyrimidine photodimers and thus decrease their bypass frequency. These results suggest that the beta 2 subunit might act as a sensor for obstacles to replication caused by DNA damage, and that it terminates elongation at these sites by promoting dissociation. The intracellular concentration of beta 2 was estimated to be 250 nM

  7. Enhancer SINEs Link Pol III to Pol II Transcription in Neurons

    Directory of Open Access Journals (Sweden)

    Cristina Policarpi

    2017-12-01

    Full Text Available Summary: Spatiotemporal regulation of gene expression depends on the cooperation of multiple mechanisms, including the functional interaction of promoters with distally located enhancers. Here, we show that, in cortical neurons, a subset of short interspersed nuclear elements (SINEs located in the proximity of activity-regulated genes bears features of enhancers. Enhancer SINEs (eSINEs recruit the Pol III cofactor complex TFIIIC in a stimulus-dependent manner and are transcribed by Pol III in response to neuronal depolarization. Characterization of an eSINE located in proximity to the Fos gene (FosRSINE1 indicated that the FosRSINE1-encoded transcript interacts with Pol II at the Fos promoter and mediates Fos relocation to Pol II factories, providing an unprecedented molecular link between Pol III and Pol II transcription. Strikingly, knockdown of the FosRSINE1 transcript induces defects of both cortical radial migration in vivo and activity-dependent dendritogenesis in vitro, demonstrating that FosRSINE1 acts as a strong enhancer of Fos expression in diverse physiological contexts. : Spatiotemporal regulation of gene expression requires the interaction between promoters and distally located enhancers. Policarpi et al. identify a subset of SINEs that functions as enhancers for activity-dependent neuronal genes. The enhancer SINE FosRSINE1 regulates Fos transcription and is necessary for both activity-dependent dendritogenesis and proper brain development. Keywords: neuroscience, epigenetics, transcription, enhancers, SINEs, neuronal activity, neuronal development

  8. File list: Pol.Neu.50.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.50.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Neural ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.50.RNA_Polymerase_III.AllCell.bed ...

  9. File list: Pol.Bld.20.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bld.20.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Blood h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Bld.20.RNA_Polymerase_III.AllCell.bed ...

  10. File list: Pol.Plc.50.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Plc.50.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Placent...a http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Plc.50.RNA_Polymerase_III.AllCell.bed ...

  11. File list: Pol.Myo.10.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Myo.10.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Muscle ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Myo.10.RNA_Polymerase_III.AllCell.bed ...

  12. File list: Pol.Lar.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Lar.50.RNA_polymerase_III.AllCell ce10 RNA polymerase RNA polymerase III Larvae... http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Lar.50.RNA_polymerase_III.AllCell.bed ...

  13. File list: Pol.Oth.20.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.20.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Others ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.20.RNA_Polymerase_III.AllCell.bed ...

  14. File list: Pol.CDV.20.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.20.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Cardiov...ascular http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.20.RNA_Polymerase_III.AllCell.bed ...

  15. File list: Pol.Adp.20.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adp.20.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Adipocy...te http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Adp.20.RNA_Polymerase_III.AllCell.bed ...

  16. File list: Pol.Adl.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.05.RNA_polymerase_III.AllCell ce10 RNA polymerase RNA polymerase III Adult ...SRX395531,SRX331268,SRX331270,SRX395532 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Adl.05.RNA_polymerase_III.AllCell.bed ...

  17. File list: Pol.Oth.05.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.05.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Others ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.05.RNA_Polymerase_III.AllCell.bed ...

  18. File list: Pol.Myo.05.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Myo.05.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Muscle ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Myo.05.RNA_Polymerase_III.AllCell.bed ...

  19. File list: Pol.Pan.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Pan.05.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Pancre...as http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Pan.05.RNA_polymerase_III.AllCell.bed ...

  20. File list: Pol.CDV.05.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.05.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Cardiov...ascular http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.05.RNA_Polymerase_III.AllCell.bed ...

  1. File list: Pol.Utr.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Uterus... SRX018606,SRX017002,SRX017001 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.20.RNA_polymerase_III.AllCell.bed ...

  2. File list: Pol.Epd.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Epd.10.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Epider...mis SRX016997 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Epd.10.RNA_polymerase_III.AllCell.bed ...

  3. File list: Pol.Brs.50.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Brs.50.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Breast ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Brs.50.RNA_Polymerase_III.AllCell.bed ...

  4. File list: Pol.Lar.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Lar.20.RNA_polymerase_III.AllCell ce10 RNA polymerase RNA polymerase III Larvae... http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Lar.20.RNA_polymerase_III.AllCell.bed ...

  5. File list: Pol.Unc.10.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.10.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Unclass...ified http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Unc.10.RNA_Polymerase_III.AllCell.bed ...

  6. File list: Pol.ALL.20.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.20.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III All cel...l types ERX204069 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.ALL.20.RNA_Polymerase_III.AllCell.bed ...

  7. File list: Pol.Unc.50.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.50.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Unclass...ified http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Unc.50.RNA_Polymerase_III.AllCell.bed ...

  8. File list: Pol.Dig.05.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.05.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Digesti...ve tract http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.05.RNA_Polymerase_III.AllCell.bed ...

  9. File list: Pol.Dig.50.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.50.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Digesti...ve tract http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.50.RNA_Polymerase_III.AllCell.bed ...

  10. File list: Pol.Lng.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Lng.05.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Lung S...RX016555,SRX150101,SRX150102 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Lng.05.RNA_polymerase_III.AllCell.bed ...

  11. File list: Pol.CDV.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.10.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Cardio...vascular http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.CDV.10.RNA_polymerase_III.AllCell.bed ...

  12. File list: Pol.Unc.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.50.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Unclas...sified http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Unc.50.RNA_polymerase_III.AllCell.bed ...

  13. File list: Pol.ALL.50.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.50.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III All cel...l types ERX204069 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.ALL.50.RNA_Polymerase_III.AllCell.bed ...

  14. File list: Pol.Plc.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Plc.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Placen...ta http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Plc.20.RNA_polymerase_III.AllCell.bed ...

  15. File list: Pol.Bon.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bon.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Bone h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Bon.20.RNA_polymerase_III.AllCell.bed ...

  16. File list: Pol.Emb.05.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.05.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Embryo ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Emb.05.RNA_Polymerase_III.AllCell.bed ...

  17. File list: Pol.Emb.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.10.RNA_polymerase_III.AllCell ce10 RNA polymerase RNA polymerase III Embryo... http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Emb.10.RNA_polymerase_III.AllCell.bed ...

  18. File list: Pol.Pan.10.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Pan.10.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Pancrea...s http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Pan.10.RNA_Polymerase_III.AllCell.bed ...

  19. File list: Pol.Bon.05.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bon.05.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Bone ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Bon.05.RNA_Polymerase_III.AllCell.bed ...

  20. File list: Pol.Adp.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adp.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Adipoc...yte http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Adp.20.RNA_polymerase_III.AllCell.bed ...

  1. File list: Pol.Spl.10.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Spl.10.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III Spleen ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Spl.10.RNA_Polymerase_III.AllCell.bed ...

  2. File list: Pol.Adp.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adp.10.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Adipoc...yte http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Adp.10.RNA_polymerase_III.AllCell.bed ...

  3. File list: Pol.Dig.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Digest...ive tract http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Dig.20.RNA_polymerase_III.AllCell.bed ...

  4. File list: Pol.Myo.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Myo.10.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Muscle... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Myo.10.RNA_polymerase_III.AllCell.bed ...

  5. File list: Pol.Epd.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Epd.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Epider...mis SRX016997 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Epd.20.RNA_polymerase_III.AllCell.bed ...

  6. File list: Pol.Plc.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Plc.10.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Placen...ta http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Plc.10.RNA_polymerase_III.AllCell.bed ...

  7. File list: Pol.Brs.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Brs.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Breast... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Brs.20.RNA_polymerase_III.AllCell.bed ...

  8. File list: Pol.Brs.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Brs.05.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Breast... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Brs.05.RNA_polymerase_III.AllCell.bed ...

  9. File list: Pol.PSC.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Plurip...otent stem cell http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.05.RNA_polymerase_III.AllCell.bed ...

  10. File list: Pol.Prs.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Prs.10.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Prosta...te http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Prs.10.RNA_polymerase_III.AllCell.bed ...

  11. File list: Pol.Oth.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.50.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Others... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Oth.50.RNA_polymerase_III.AllCell.bed ...

  12. File list: Pol.Utr.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.10.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Uterus... SRX017001,SRX018606,SRX017002 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.10.RNA_polymerase_III.AllCell.bed ...

  13. File list: Pol.CDV.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.05.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Cardio...vascular http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.CDV.05.RNA_polymerase_III.AllCell.bed ...

  14. File list: Pol.Brs.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Brs.10.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Breast... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Brs.10.RNA_polymerase_III.AllCell.bed ...

  15. File list: Pol.Kid.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  16. File list: Pol.ALL.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  17. File list: Pol.Liv.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  18. File list: Pol.Myo.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  19. File list: Pol.Lng.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  20. File list: Pol.Kid.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  1. File list: Pol.ALL.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  2. File list: Pol.Dig.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  3. File list: Pol.Oth.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  4. File list: Pol.Lar.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  5. File list: Pol.Liv.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  6. File list: Pol.Gon.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  7. File list: Pol.Bld.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  8. File list: Pol.ALL.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  9. File list: Pol.Plc.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  10. File list: Pol.Lng.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  11. File list: Pol.Emb.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  12. File list: Pol.Oth.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  13. File list: Pol.Bon.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  14. File list: Pol.Unc.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  15. File list: Pol.Gon.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  16. File list: Pol.Emb.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  17. File list: Pol.PSC.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  18. File list: Pol.Adl.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  19. File list: Pol.Myo.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  20. File list: Pol.Plc.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  1. File list: Pol.Lar.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  2. File list: Pol.Bon.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  3. File list: Pol.Oth.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  4. File list: Pol.Kid.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  5. File list: Pol.CDV.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  6. File list: Pol.Prs.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  7. File list: Pol.Bld.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  8. File list: Pol.Emb.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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    Full Text Available Pol.Emb.20.RNA_polymerase_III.AllCell ce10 RNA polymerase RNA polymerase III Embryo... http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Emb.20.RNA_polymerase_III.AllCell.bed ...

  9. File list: Pol.Epd.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  10. File list: Pol.Utr.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  11. File list: Pol.Kid.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  12. File list: Pol.Pan.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  13. File list: Pol.Adl.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  14. File list: Pol.Neu.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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  15. File list: Pol.Myo.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  16. File list: Pol.Liv.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Liv.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Liver ...http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Liv.20.RNA_polymerase_III.AllCell.bed ...

  17. File list: Pol.Gon.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Gon.50.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Gonad ...http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Gon.50.RNA_polymerase_III.AllCell.bed ...

  18. Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme*

    Science.gov (United States)

    Sato, Shigeo; Tomomori-Sato, Chieri; Tsai, Kuang-Lei; Yu, Xiaodi; Sardiu, Mihaela; Saraf, Anita; Washburn, Michael P.; Florens, Laurence; Asturias, Francisco J.; Conaway, Ronald C.

    2016-01-01

    Mediator plays an integral role in activation of RNA polymerase II (Pol II) transcription. A key step in activation is binding of Mediator to Pol II to form the Mediator-Pol II holoenzyme. Here, we exploit a combination of biochemistry and macromolecular EM to investigate holoenzyme assembly. We identify a subset of human Mediator head module subunits that bind Pol II independent of other subunits and thus probably contribute to a major Pol II binding site. In addition, we show that binding of human Mediator to Pol II depends on the integrity of a conserved “hinge” in the middle module MED21-MED7 heterodimer. Point mutations in the hinge region leave core Mediator intact but lead to increased disorder of the middle module and markedly reduced affinity for Pol II. These findings highlight the importance of Mediator conformation for holoenzyme assembly. PMID:27821593

  19. File list: Pol.NoD.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.NoD.50.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III No des...cription http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.NoD.50.RNA_polymerase_III.AllCell.bed ...

  20. File list: Pol.NoD.10.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  1. File list: Pol.NoD.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.NoD.20.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III No des...cription http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.NoD.20.RNA_polymerase_III.AllCell.bed ...

  2. Alteration of BRCA1 expression affects alcohol-induced transcription of RNA Pol III-dependent genes.

    Science.gov (United States)

    Zhong, Qian; Shi, Ganggang; Zhang, Yanmei; Lu, Lei; Levy, Daniel; Zhong, Shuping

    2015-02-01

    Emerging evidence has indicated that alcohol consumption is an established risk factor for breast cancer. Deregulation of RNA polymerase III (Pol III) transcription enhances cellular Pol III gene production, leading to an increase in translational capacity to promote cell transformation and tumor formation. We have reported that alcohol intake increases Pol III gene transcription to promote cell transformation and tumor formation in vitro and in vivo. Studies revealed that tumor suppressors, pRb, p53, PTEN and Maf1 repress the transcription of Pol III genes. BRCA1 is a tumor suppressor and its mutation is tightly related to breast cancer development. However, it is not clear whether BRCA1 expression affects alcohol-induced transcription of Pol III genes. At the present studies, we report that restoring BRCA1 in HCC 1937 cells, which is a BRCA1 deficient cell line, represses Pol III gene transcription. Expressing mutant or truncated BRCA1 in these cells does not affect the ability of repression on Pol III genes. Our analysis has demonstrated that alcohol induces Pol III gene transcription. More importantly, overexpression of BRCA1 in estrogen receptor positive (ER+) breast cancer cells (MCF-7) decreases the induction of tRNA(Leu) and 5S rRNA genes by alcohol, whereas reduction of BRCA1 by its siRNA slightly increases the transcription of the class of genes. This suggests that BRCA1 is associated with alcohol-induced deregulation of Pol III genes. These studies for the first time demonstrate the role of BRCA1 in induction of Pol III genes by alcohol and uncover a novel mechanism of alcohol-associated breast cancer. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Crystal Structure and Regulation of the Citrus Pol III Repressor MAF1 by Auxin and Phosphorylation.

    Science.gov (United States)

    Soprano, Adriana Santos; Giuseppe, Priscila Oliveira de; Shimo, Hugo Massayoshi; Lima, Tatiani Brenelli; Batista, Fernanda Aparecida Heleno; Righetto, Germanna Lima; Pereira, José Geraldo de Carvalho; Granato, Daniela Campos; Nascimento, Andrey Fabricio Ziem; Gozzo, Fabio Cesar; de Oliveira, Paulo Sérgio Lopes; Figueira, Ana Carolina Migliorini; Smetana, Juliana Helena Costa; Paes Leme, Adriana Franco; Murakami, Mario Tyago; Benedetti, Celso Eduardo

    2017-09-05

    MAF1 is the main RNA polymerase (Pol) III repressor that controls cell growth in eukaryotes. The Citrus ortholog, CsMAF1, was shown to restrict cell growth in citrus canker disease but its role in plant development and disease is still unclear. We solved the crystal structure of the globular core of CsMAF1, which reveals additional structural elements compared with the previously available structure of hMAF1, and explored the dynamics of its flexible regions not present in the structure. CsMAF1 accumulated in the nucleolus upon leaf excision, and this translocation was inhibited by auxin and by mutation of the PKA phosphorylation site, S45, to aspartate. Additionally, mTOR phosphorylated recombinant CsMAF1 and the mTOR inhibitor AZD8055 blocked canker formation in normal but not CsMAF1-silenced plants. These results indicate that the role of TOR on cell growth induced by Xanthomonas citri depends on CsMAF1 and that auxin controls CsMAF1 interaction with Pol III in citrus. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. La escuela de Mira-El-Río: su significado en la política educativa de Carlos III

    Directory of Open Access Journals (Sweden)

    Paloma PERNIL ALARCÓN

    2010-03-01

    Full Text Available RESUMEN: La personalidad de Carlos III exige ser traducida más en el significado de sus acciones políticas de reforma social que en la existencia de grandes gestos de actitudes absolutistas personales. La problemática planteada a la Corona se hace patente en la búsqueda de soluciones a través de las reformas exigidas y de los organismos que se crean como exigencia de una eficacia mediante una política de integración y de colaboración estamental.

  5. CMG helicase and DNA polymerase ε form a functional 15-subunit holoenzyme for eukaryotic leading-strand DNA replication.

    Science.gov (United States)

    Langston, Lance D; Zhang, Dan; Yurieva, Olga; Georgescu, Roxana E; Finkelstein, Jeff; Yao, Nina Y; Indiani, Chiara; O'Donnell, Mike E

    2014-10-28

    DNA replication in eukaryotes is asymmetric, with separate DNA polymerases (Pol) dedicated to bulk synthesis of the leading and lagging strands. Pol α/primase initiates primers on both strands that are extended by Pol ε on the leading strand and by Pol δ on the lagging strand. The CMG (Cdc45-MCM-GINS) helicase surrounds the leading strand and is proposed to recruit Pol ε for leading-strand synthesis, but to date a direct interaction between CMG and Pol ε has not been demonstrated. While purifying CMG helicase overexpressed in yeast, we detected a functional complex between CMG and native Pol ε. Using pure CMG and Pol ε, we reconstituted a stable 15-subunit CMG-Pol ε complex and showed that it is a functional polymerase-helicase on a model replication fork in vitro. On its own, the Pol2 catalytic subunit of Pol ε is inefficient in CMG-dependent replication, but addition of the Dpb2 protein subunit of Pol ε, known to bind the Psf1 protein subunit of CMG, allows stable synthesis with CMG. Dpb2 does not affect Pol δ function with CMG, and thus we propose that the connection between Dpb2 and CMG helps to stabilize Pol ε on the leading strand as part of a 15-subunit leading-strand holoenzyme we refer to as CMGE. Direct binding between Pol ε and CMG provides an explanation for specific targeting of Pol ε to the leading strand and provides clear mechanistic evidence for how strand asymmetry is maintained in eukaryotes.

  6. Functional characterization of Pol III U6 promoters for gene knockdown and knockout in Plutella xylostella.

    Science.gov (United States)

    Huang, Yuping; Wang, Yajun; Zeng, Baosheng; Liu, Zhaoxia; Xu, Xuejiao; Meng, Qian; Huang, Yongping; Yang, Guang; Vasseur, Liette; Gurr, Geoff M; You, Minsheng

    2017-10-01

    RNA polymerase type III (Pol-III) promoters such as U6 are commonly used to express small RNAs, including short hairpin RNAs (shRNAs) and single guide RNAs (sgRNAs). Functional U6 promoters are widely used in CRISPR systems, and their characterization can facilitate genome editing of non-model organisms. In the present study, six U6 small nuclear RNA (snRNA) promoters containing two conserved elements of a proximal sequence element (PSEA) and a TATA box, were identified and characterized in the diamondback moth (Plutella xylostella) genome. Relative efficiency of the U6 promoters to express shRNA induced EGFP knockdown was tested in a P. xylostella cell line, revealing that the PxU6:3 promoter had the strongest expression effect. Further work with the PxU6:3 promoter showed its efficacy in EGFP knockout using CRISPR/Cas9 system in the cells. The expression plasmids with versatile Pxabd-A gene specific sgRNA driven by the PxU6:3 promoter, combined with Cas9 mRNA, could induce mutagenesis at specific genomic loci in vivo. The phenotypes induced by sgRNA expression plasmids were similar to those done in vitro transcription sgRNAs. A plasmid with two tandem arranged PxU6:3:sgRNA expression cassettes targeting Pxabd-A loci was generated, which caused a 28,856 bp fragment deletion, suggesting that the multi-sgRNA expression plasmid can be used for multi-targeting. Our work indicates that U6 snRNA promoters can be used for functional studies of genes with the approach of reverse genetics in P. xylostella. These essential promoters also provide valuable potential for CRISPR-derived gene drive as a tactic for population control in this globally significant pest. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode

    KAUST Repository

    Jergic, Slobodan; Horan, Nicholas P.; Elshenawy, Mohamed; Mason, Claire E.; Urathamakul, Thitima; Ozawa, Kiyoshi; Robinson, Andrew J.; Goudsmits, Joris M H; Wang, Yao; Pan, Xuefeng; Beck, Jennifer L.; Van Oijen, Antoine M.; Huber, Thomas L.; Hamdan, Samir; Dixon, Nicholas E.

    2013-01-01

    Processive DNA synthesis by the αÉ"θ core of the Escherichia coli Pol III replicase requires it to be bound to the β 2 clamp via a site in the α polymerase subunit. How the É" proofreading exonuclease subunit influences DNA synthesis by α was not previously understood. In this work, bulk assays of DNA replication were used to uncover a non-proofreading activity of É". Combination of mutagenesis with biophysical studies and single-molecule leading-strand replication assays traced this activity to a novel β-binding site in É" that, in conjunction with the site in α, maintains a closed state of the αÉ"θ-β 2 replicase in the polymerization mode of DNA synthesis. The É"-β interaction, selected during evolution to be weak and thus suited for transient disruption to enable access of alternate polymerases and other clamp binding proteins, therefore makes an important contribution to the network of protein-protein interactions that finely tune stability of the replicase on the DNA template in its various conformational states. © 2013 European Molecular Biology Organization.

  8. A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode

    KAUST Repository

    Jergic, Slobodan

    2013-02-22

    Processive DNA synthesis by the αÉ"θ core of the Escherichia coli Pol III replicase requires it to be bound to the β 2 clamp via a site in the α polymerase subunit. How the É" proofreading exonuclease subunit influences DNA synthesis by α was not previously understood. In this work, bulk assays of DNA replication were used to uncover a non-proofreading activity of É". Combination of mutagenesis with biophysical studies and single-molecule leading-strand replication assays traced this activity to a novel β-binding site in É" that, in conjunction with the site in α, maintains a closed state of the αÉ"θ-β 2 replicase in the polymerization mode of DNA synthesis. The É"-β interaction, selected during evolution to be weak and thus suited for transient disruption to enable access of alternate polymerases and other clamp binding proteins, therefore makes an important contribution to the network of protein-protein interactions that finely tune stability of the replicase on the DNA template in its various conformational states. © 2013 European Molecular Biology Organization.

  9. Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.

    Science.gov (United States)

    Ahuja, Richa; Kumar, Vijay

    2017-07-01

    RNA polymerase III transcribes structurally diverse group of essential noncoding RNAs including 5S ribosomal RNA (5SrRNA) and U6 snRNA. These noncoding RNAs are involved in RNA processing and ribosome biogenesis, thus, coupling Pol III activity to the rate of protein synthesis, cell growth, and proliferation. Even though a few Pol II-associated transcription factors have been reported to participate in Pol III-dependent transcription, its activation by activator protein 1 (AP-1) factors, c-Fos and c-Jun, has remained unexplored. Here, we show that c-Fos and c-Jun bind to specific sites in the regulatory regions of 5S rRNA (type I) and U6 snRNA (type III) gene promoters and stimulate their transcription. Our chromatin immunoprecipitation studies suggested that endogenous AP-1 factors bind to their cognate promoter elements during the G1/S transition of cell cycle apparently synchronous with Pol III transcriptional activity. Furthermore, the interaction of c-Jun with histone acetyltransferase p300 promoted the recruitment of p300/CBP complex on the promoters and facilitated the occupancy of Pol III transcriptional machinery via histone acetylation and chromatin remodeling. The findings of our study, together, suggest that AP-1 factors are novel regulators of Pol III-driven 5S rRNA and U6 snRNA expression with a potential role in cell proliferation. © 2017 Federation of European Biochemical Societies.

  10. O marxismo indo-americano de Mariátegui e a polêmica com a III Internacional Comunista.

    Directory of Open Access Journals (Sweden)

    María Gabriela Guillén

    2012-05-01

    Full Text Available Partindo da perspectiva marxista indo-americana de José Carlos Mariátegui, o artigo visa a exposição de fatores teóricos e históricos que culminaram na polêmica com a III Internacional Comunista na América Latina. A problemática evidencia a originalidade do pensamento de Mariátegui, cujo eixo central é a interpretação dos fatos históricos, políticos e sociais latino-americanos sob a perspectiva dialética do desenvolvimento desigual e combinado para explicar a dinâmica particular da realidade peruana.

  11. Distinct co-evolution patterns of genes associated to DNA polymerase III DnaE and PolC

    Directory of Open Access Journals (Sweden)

    Engelen Stefan

    2012-02-01

    Full Text Available Abstract Background Bacterial genomes displaying a strong bias between the leading and the lagging strand of DNA replication encode two DNA polymerases III, DnaE and PolC, rather than a single one. Replication is a highly unsymmetrical process, and the presence of two polymerases is therefore not unexpected. Using comparative genomics, we explored whether other processes have evolved in parallel with each polymerase. Results Extending previous in silico heuristics for the analysis of gene co-evolution, we analyzed the function of genes clustering with dnaE and polC. Clusters were highly informative. DnaE co-evolves with the ribosome, the transcription machinery, the core of intermediary metabolism enzymes. It is also connected to the energy-saving enzyme necessary for RNA degradation, polynucleotide phosphorylase. Most of the proteins of this co-evolving set belong to the persistent set in bacterial proteomes, that is fairly ubiquitously distributed. In contrast, PolC co-evolves with RNA degradation enzymes that are present only in the A+T-rich Firmicutes clade, suggesting at least two origins for the degradosome. Conclusion DNA replication involves two machineries, DnaE and PolC. DnaE co-evolves with the core functions of bacterial life. In contrast PolC co-evolves with a set of RNA degradation enzymes that does not derive from the degradosome identified in gamma-Proteobacteria. This suggests that at least two independent RNA degradation pathways existed in the progenote community at the end of the RNA genome world.

  12. Characterization of CK2 holoenzyme variants with regard to crystallization

    DEFF Research Database (Denmark)

    Guerra, B; Niefind, K; Ermakowa, I

    2001-01-01

    severely. One of the approaches chosen was the production of a chimeric holoenzyme made up from a human regulatory subunit and a catalytic subunit from Z. mays. The plant catalytic subunit, in contrast to the human counterpart is very stable and does not undergo this kind of degradation. The second...

  13. Strand displacement by DNA polymerase III occurs through a tau-psi-chi link to single-stranded DNA-binding protein coating the lagging strand template.

    Science.gov (United States)

    Yuan, Quan; McHenry, Charles S

    2009-11-13

    In addition to the well characterized processive replication reaction catalyzed by the DNA polymerase III holoenzyme on single-stranded DNA templates, the enzyme possesses an intrinsic strand displacement activity on flapped templates. The strand displacement activity is distinguished from the single-stranded DNA-templated reaction by a high dependence upon single-stranded DNA binding protein and an inability of gamma-complex to support the reaction in the absence of tau. However, if gamma-complex is present to load beta(2), a truncated tau protein containing only domains III-V will suffice. This truncated protein is sufficient to bind both the alpha subunit of DNA polymerase (Pol) III and chipsi. This is reminiscent of the minimal requirements for Pol III to replicate short single-stranded DNA-binding protein (SSB)-coated templates where tau is only required to serve as a scaffold to hold Pol III and chi in the same complex (Glover, B., and McHenry, C. (1998) J. Biol. Chem. 273, 23476-23484). We propose a model in which strand displacement by DNA polymerase III holoenzyme depends upon a Pol III-tau-psi-chi-SSB binding network, where SSB is bound to the displaced strand, stabilizing the Pol III-template interaction. The same interaction network is probably important for stabilizing the leading strand polymerase interactions with authentic replication forks. The specificity constant (k(cat)/K(m)) for the strand displacement reaction is approximately 300-fold less favorable than reactions on single-stranded templates and proceeds with a slower rate (150 nucleotides/s) and only moderate processivity (approximately 300 nucleotides). PriA, the initiator of replication restart on collapsed or misassembled replication forks, blocks the strand displacement reaction, even if added to an ongoing reaction.

  14. Mediator structure and rearrangements required for holoenzyme formation.

    Science.gov (United States)

    Tsai, Kuang-Lei; Yu, Xiaodi; Gopalan, Sneha; Chao, Ti-Chun; Zhang, Ying; Florens, Laurence; Washburn, Michael P; Murakami, Kenji; Conaway, Ronald C; Conaway, Joan W; Asturias, Francisco J

    2017-04-13

    The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals.

  15. Metáforas de la multitud. III Congreso Internacional Estética y Política

    OpenAIRE

    Corella Lacasa, Miguel; García Puchades, Wenceslao

    2015-01-01

    Con la crisis del Estado-nación, de los imaginarios colectivos de la modernidad y del sistema de representación política partidista, un fantasma recorre el mundo entero sin acabar de tomar rostro. Una multitud de anónimos irrumpe en la escena social y política generando nuevas formas de organización ciudadana vinculadas, entre otros factores, a las posibilidades abiertas por la web 2.0. El Tercer Congreso Internacional Estética y Política se propone estudiar estas Metáforas de la multitud...

  16. Biochemical characterization of CK2alpha and alpha' paralogues and their derived holoenzymes: evidence for the existence of a heterotrimeric CK2alpha'-holoenzyme forming trimeric complexes

    DEFF Research Database (Denmark)

    Olsen, Birgitte; Rasmussen, Tine; Niefind, Karsten

    2008-01-01

    Altogether 2 holoenzymes and 4 catalytic CK2 constructs were expressed and characterized i.e. CK2alpha (2) (1-335) beta(2); CK2alpha'-derived holoenzyme; CK2alpha(1-335); MBP-CK2alpha'; His-tagged CK2alpha and His-tagged CK2alpha'. The two His-tagged catalytic subunits were expressed in insect...... cells, all others in Escherichia coli. IC(50) studies involving the established CK2 inhibitors DMAT, TBBt, TBBz, apigenin and emodin were carried out and the K(i) values calculated. Although the differences in the K(i) values found were modest, there was a general tendency showing that the CK2...... holoenzymes were more sensitive towards the inhibitors than the free catalytic subunits. Thermal inactivation experiments involving the individual catalytic subunits showed an almost complete loss of activity after only 2 min at 45 degrees C. In the case of the two holoenzymes, the CK2alpha...

  17. ChIP-exo interrogation of Crp, DNA, and RNAP holoenzyme interactions

    DEFF Research Database (Denmark)

    Latif, Haythem; Federowicz, Stephen; Ebrahim, Ali

    2018-01-01

    Numerous in vitro studies have yielded a refined picture of the structural and molecular associations between Cyclic-AMP receptor protein (Crp), the DNA motif, and RNA polymerase (RNAP) holoenzyme. In this study, high-resolution ChIP-exonuclease (ChIP-exo) was applied to study Crp binding in vivo...

  18. Ciliate telomerase RNA loop IV nucleotides promote hierarchical RNP assembly and holoenzyme stability.

    Science.gov (United States)

    Robart, Aaron R; O'Connor, Catherine M; Collins, Kathleen

    2010-03-01

    Telomerase adds simple-sequence repeats to chromosome 3' ends to compensate for the loss of repeats with each round of genome replication. To accomplish this de novo DNA synthesis, telomerase uses a template within its integral RNA component. In addition to providing the template, the telomerase RNA subunit (TER) also harbors nontemplate motifs that contribute to the specialized telomerase catalytic cycle of reiterative repeat synthesis. Most nontemplate TER motifs function through linkage with the template, but in ciliate and vertebrate telomerases, a stem-loop motif binds telomerase reverse transcriptase (TERT) and reconstitutes full activity of the minimal recombinant TERT+TER RNP, even when physically separated from the template. Here, we resolve the functional requirements for this motif of ciliate TER in physiological RNP context using the Tetrahymena thermophila p65-TER-TERT core RNP reconstituted in vitro and the holoenzyme reconstituted in vivo. Contrary to expectation based on assays of the minimal recombinant RNP, we find that none of a panel of individual loop IV nucleotide substitutions impacts the profile of telomerase product synthesis when reconstituted as physiological core RNP or holoenzyme RNP. However, loop IV nucleotide substitutions do variably reduce assembly of TERT with the p65-TER complex in vitro and reduce the accumulation and stability of telomerase RNP in endogenous holoenzyme context. Our results point to a unifying model of a conformational activation role for this TER motif in the telomerase RNP enzyme.

  19. Crystal structure of the 500-kDa yeast acetyl-CoA carboxylase holoenzyme dimer

    Energy Technology Data Exchange (ETDEWEB)

    Wei, Jia; Tong, Liang

    2015-10-12

    Acetyl-CoA carboxylase (ACC) has crucial roles in fatty acid metabolism and is an attractive target for drug discovery against diabetes, cancer and other diseases1, 2, 3, 4, 5, 6. Saccharomyces cerevisiae ACC (ScACC) is crucial for the production of very-long-chain fatty acids and the maintenance of the nuclear envelope7, 8. ACC contains biotin carboxylase (BC) and carboxyltransferase (CT) activities, and its biotin is linked covalently to the biotin carboxyl carrier protein (BCCP). Most eukaryotic ACCs are 250-kilodalton (kDa), multi-domain enzymes and function as homodimers and higher oligomers. They contain a unique, 80-kDa central region that shares no homology with other proteins. Although the structures of the BC, CT and BCCP domains and other biotin-dependent carboxylase holoenzymes are known1, 9, 10, 11, 12, 13, 14, there is currently no structural information on the ACC holoenzyme. Here we report the crystal structure of the full-length, 500-kDa holoenzyme dimer of ScACC. The structure is remarkably different from that of the other biotin-dependent carboxylases. The central region contains five domains and is important for positioning the BC and CT domains for catalysis. The structure unexpectedly reveals a dimer of the BC domain and extensive conformational differences compared to the structure of the BC domain alone, which is a monomer. These structural changes reveal why the BC domain alone is catalytically inactive and define the molecular mechanism for the inhibition of eukaryotic ACC by the natural product soraphen A15, 16 and by phosphorylation of a Ser residue just before the BC domain core in mammalian ACC. The BC and CT active sites are separated by 80 Å, and the entire BCCP domain must translocate during catalysis.

  20. Replication restart in UV-irradiated Escherichia coli involving pols II, III, V, PriA, RecA and RecFOR proteins.

    Science.gov (United States)

    Rangarajan, Savithri; Woodgate, Roger; Goodman, Myron F

    2002-02-01

    In Escherichia coli, UV-irradiated cells resume DNA synthesis after a transient inhibition by a process called replication restart. To elucidate the role of several key proteins involved in this process, we have analysed the time dependence of replication restart in strains carrying a combination of mutations in lexA, recA, polB (pol II), umuDC (pol V), priA, dnaC, recF, recO or recR. We find that both pol II and the origin-independent primosome-assembling function of PriA are essential for the immediate recovery of DNA synthesis after UV irradiation. In their absence, translesion replication or 'replication readthrough' occurs approximately 50 min after UV and is pol V-dependent. In a wild-type, lexA+ background, mutations in recF, recO or recR block both pathways. Similar results were obtained with a lexA(Def) recF strain. However, lexA(Def) recO or lexA(Def) recR strains, although unable to facilitate PriA-pol II-dependent restart, were able to perform pol V-dependent readthrough. The defects in restart attributed to mutations in recF, recO or recR were suppressed in a recA730 lexA(Def) strain expressing constitutively activated RecA (RecA*). Our data suggest that in a wild-type background, RecF, O and R are important for the induction of the SOS response and the formation of RecA*-dependent recombination intermediates necessary for PriA/Pol II-dependent replication restart. In con-trast, only RecF is required for the activation of RecA that leads to the formation of pol V (UmuD'2C) and facilitates replication readthrough.

  1. Sobre la decadencia económica y urbana de Gades en el contexto político del siglo III

    Directory of Open Access Journals (Sweden)

    Noé Villaverde Vega

    1997-01-01

    Full Text Available No parece casual que durante los s. i a. de C y ii de C, se atestigüe una estrecha relación de Gades con personajes de las más altas esferas políticas romanas, pues durante dicho periodo esta localidad, obtuvo una notable expansión al amparo de los beneficios fiscales otorgados por el Estado romano. Por contra, el ascenso al poder de la Dinastía Severiana, caracterizado por una despiadada aniquilación de sus oponentes, entre los cuales destacaba la aristocracia bélica prácticamente en pleno, parece haber determinado la pérdida de influencias hasta entonces detentadas por el ámbito hispánico meridional en la cancillería romana. En consecuencia a fines del s. II o principios del s. III, se advierte la repercusión negativa que la nueva normativa severíana tendrían sobre las localidades costeras del fretum gaditamum, quienes súbitamente privadas de privilegios que garantizaban su prosperidad, fueron obligadas a una estricta reconversión recesiva; todo se evidencia con particular gravedad en Gades, localidad que en el transcurso del s. III resultaría completamente arruinada, llegando incluso a perder su, acaso milenaria, categoría urbana.It's evident the conexión of Gades with the most influential dignataries of the highest politicat spheres after its adhesión to Rome, especially from V-' century BC to 2nd AD when the southern Híspanla shipping trade, from which Gades the main exponent, got a substantial expansión under the projection of tax allowances given by the Román government. Conversely, Severian Dinasty's hse to power. charactehzed by a merciless destruction of every oponent among whom nearly all the baetican ahstocracy were, seens to have caused the loss of power commanded up till the by the Southern Hispania área, consequently of Gades, on the Román chancellery's resolutions. Accordingly Severían policy about shipiing and taxation appears to have implied a deep economical «crisis» for the Strait territory

  2. The AAA-ATPase NVL2 is a telomerase component essential for holoenzyme assembly

    Energy Technology Data Exchange (ETDEWEB)

    Her, Joonyoung [Departments of Biology and Integrated Omics for Biomedical Science, Yonsei University, Seoul 120-749 (Korea, Republic of); Chung, In Kwon, E-mail: topoviro@yonsei.ac.kr [Departments of Biology and Integrated Omics for Biomedical Science, Yonsei University, Seoul 120-749 (Korea, Republic of)

    2012-01-20

    Highlights: Black-Right-Pointing-Pointer Identification of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. Black-Right-Pointing-Pointer NVL2 associates with catalytically active telomerase via an interaction with hTERT. Black-Right-Pointing-Pointer NVL2 is a telomerase component essential for holoenzyme assembly. Black-Right-Pointing-Pointer ATP-binding activity of NVL2 is required for hTERT binding and telomerase assembly. -- Abstract: Continued cell proliferation requires telomerase to maintain functional telomeres that are essential for chromosome integrity. Although the core enzyme includes a telomerase reverse transcriptase (TERT) and a telomerase RNA component (TERC), a number of auxiliary proteins have been identified to regulate telomerase assembly, localization, and enzymatic activity. Here we describe the characterization of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. NVL2 interacts and co-localizes with hTERT in the nucleolus. NLV2 is also found in association with catalytically competent telomerase in cell lysates through an interaction with hTERT. Depletion of endogenous NVL2 by small interfering RNA led to a decrease in hTERT without affecting the steady-state levels of hTERT mRNA, thereby reducing telomerase activity, suggesting that NVL2 is an essential component of the telomerase holoenzyme. We also found that ATP-binding activity of NVL2 is required for hTERT binding as well as telomerase assembly. Our findings suggest that NVL2, in addition to its role in ribosome biosynthesis, is essential for telomerase biogenesis and provides an alternative approach for inhibiting telomerase activity in cancer.

  3. Factor C*, the specific initiation component of the mouse RNA polymerase I holoenzyme, is inactivated early in the transcription process.

    OpenAIRE

    Brun, R P; Ryan, K; Sollner-Webb, B

    1994-01-01

    Factor C* is the component of the RNA polymerase I holoenzyme (factor C) that allows specific transcriptional initiation on a factor D (SL1)- and UBF-activated rRNA gene promoter. The in vitro transcriptional capacity of a preincubated rDNA promoter complex becomes exhausted very rapidly upon initiation of transcription. This is due to the rapid depletion of C* activity. In contrast, C* activity is not unstable in the absence of transcription, even in the presence of nucleoside triphosphates ...

  4. A difícil arte de "deslizar sobre o mundo" ou Montaigne, um político discreto ("De poupar a própria vontade" - III, 10

    Directory of Open Access Journals (Sweden)

    Andre Scoralick

    2012-12-01

    Full Text Available De 1581 a 1585, Montaigne foi prefeito de Bordeaux. Foi acusado por detratores de não se ter aplicado o bastante e de não ter feito nada de marcante durante seus dois mandatos. Ao responder às acusações no ensaio "De poupar a própria vontade" (III, 10, o autor encontra a ocasião para uma crítica das paixões em geral e, em particular, das que pertencem ao contexto político. Isto porque ele visava, com sua aparente falta de aplicação aos deveres de prefeito, evitar a paixão que tantas vezes se oculta por trás do engajamento - a ambição, desejo de honras, glória, renome. Sobretudo, esperava evitar um duplo perigo, ao mesmo tempo ético e político: comprometer a própria liberdade numa busca servil da glória e subordinar o bem coletivo ao interesse pessoal. No presente estudo, procuraremos reconstituir a trama argumentativa do ensaio em questão, acompanhando de perto os argumentos que sustentam a crítica montaigniana das paixões, bem como a terapia muito particular a que o ensaísta as submete, a qual lhe permite fazer do episódio da Mairie de Bordeaux um modelo de conciliação entre o cuidado de si e o cumprimento dos deveres políticos.From 1581 to 1585 Montaigne was the mayor of Bordeaux. He was accused by detractors of not having been sufficiently dedicated to his functions and of having done nothing remarkable during his two terms. In answering to such charges in the essay "Of Managing the Will" (III, 10, the author finds the occasion for criticising passions in general and, in particular, those concerning the political context. The reason for it is that he sought, with his apparent lack of commitment to the mayor's duties, to avoid the passions which usually hide behind such an engagement: ambition, desire for honours, glory, renown. Above all he hoped to avoid a double danger, both ethical and political: to compromise his freedom in a servile pursuit of glory and to subordinate the common good to his personal advantage

  5. Comparison of the kinetic parameters of the truncated catalytic subunit and holoenzyme of human DNA polymerase ε

    Science.gov (United States)

    Zahurancik, Walter J.; Baranovskiy, Andrey G.; Tahirov, Tahir H.; Suo, Zucai

    2015-01-01

    Numerous genetic studies have provided compelling evidence to establish DNA polymerase ε (Polε) as the primary DNA polymerase responsible for leading strand synthesis during eukaryotic nuclear genome replication. Polε is a heterotetramer consisting of a large catalytic subunit that contains the conserved polymerase core domain as well as a 3′ → 5′ exonuclease domain common to many replicative polymerases. In addition, Polε possesses three small subunits that lack a known catalytic activity but associate with components involved in a variety of DNA replication and maintenance processes. Previous enzymatic characterization of the Polε heterotetramer from budding yeast suggested that the small subunits slightly enhance DNA synthesis by Polε in vitro. However, similar studies of the human Polε heterote-tramer (hPolε) have been limited by the difficulty of obtaining hPolε in quantities suitable for thorough investigation of its catalytic activity. Utilization of a baculovirus expression system for overexpression and purification of hPolε from insect host cells has allowed for isolation of greater amounts of active hPolε, thus enabling a more detailed kinetic comparison between hPolε and an active N-terminal fragment of the hPolε catalytic subunit (p261N), which is readily overexpressed in Escherichia coli. Here, we report the first pre-steady-state studies of fully-assembled hPolε. We observe that the small subunits increase DNA binding by hPolε relative to p261N, but do not increase processivity during DNA synthesis on a single-stranded M13 template. Interestingly, the 3′ → 5′ exonuclease activity of hPolε is reduced relative to p261N on matched and mismatched DNA substrates, indicating that the presence of the small subunits may regulate the proofreading activity of hPolε and sway hPolε toward DNA synthesis rather than proofreading. PMID:25684708

  6. The whole set of constitutive promoters recognized by RNA polymerase RpoD holoenzyme of Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Tomohiro Shimada

    Full Text Available The promoter selectivity of Escherichia coli RNA polymerase is determined by the sigma subunit with promoter recognition activity. The model prokaryote Escherichia coli contains seven species of the sigma subunit, each recognizing a specific set of promoters. The major sigma subunit, sigma-70 encoded by rpoD, plays a major role in transcription of growth-related genes. Concomitant with the increase in detection of promoters functioning in vivo under various stressful conditions, the variation is expanding in the consensus sequence of RpoD promoters. In order to identify the canonical sequence of "constitutive promoters" that are recognized by the RNA polymerase holoenzyme containing RpoD sigma in the absence of supporting transcription factors, an in vitro mixed transcription assay was carried out using a whole set of variant promoters, each harboring one base replacement, within the model promoter with the conserved -35 and -10 sequences of RpoD promoters. The consensus sequences, TTGACA(-35 and TATAAT(-10, were identified to be ideal for the maximum level of open complex formation and the highest rate of promoter opening, respectively. For identification of the full range of constitutive promoters on the E. coli genome, a total of 2,701 RpoD holoenzyme-binding sites were identified by Genomic SELEX screening, and using the reconfirmed consensus promoter sequence, a total of maximum 669 constitutive promoters were identified, implying that the majority of hitherto identified promoters represents the TF-dependent "inducible promoters". One unique feature of the constitutive promoters is the high level of promoter sequence conservation, about 85% carrying five-out-of-six agreements with -35 or -10 consensus sequence. The list of constitutive promoters provides the community resource toward estimation of the inducible promoters that operate under various stressful conditions in nature.

  7. The political-economic education of the rulers in the discourses to the king Philip III by Pedro de Valencia (1555-1620 La educación político-económica del gobernante en los discursos al rey Felipe III de Pedro de Valencia (1555-1620

    Directory of Open Access Journals (Sweden)

    Jesús Luis PARADINAS FUENTES

    2013-02-01

    Full Text Available Present-day economical crisis resembles partly that one suffered in Spain at the beginning of the 17th century. In those days, the Spanish Crown, which like all modern States had needed to carry out new functions such as deciding the ell into a deep economic crisis. Many treatises and discourses, several of them addressed to the king Philip III were written to change the economic policy of the State, which was considered responsible for the evils affecting Spain. Some of these texts have been studied by the historians of economics, but not by the historians of education, however they can be, in our opinion, included in that literary genre whose objective was the education of the princes. In this work we will analyse the writings dedicated to that king by the Spanish humanist Pedro de Valencia with the aim of educating the rulers of the Monarchy so that they could acquire the necessary intellectual and moral virtues to accomplish their duties. There are in his texts many teachings that are still valid nowadays.La crisis económica actual tiene ciertos paralelismos con la que sufrió España en el comienzo del siglo XVII. En aquel tiempo, la Corona española, que como todos los Estados modernos para poder consolidarse había tenido necesidad de cumplir nuevas funciones como las de decidir la política fiscal y la monetaria, entró en una profunda crisis económica. Con la intención de cambiar la política económica del Estado, a la que se hacía responsable de los males que aquejaban a España, se redactaron multitud de discursos y tratados, muchos de los cuales se dirigieron directamente al rey Felipe III. Algunos de estos escritos han sido estudiados por los historiadores de la economía, pero no por los de la educación, a pesar de que, según nuestra opinión, pueden incluirse dentro del género de literatura que tenía como objetivo la educación de los príncipes. En este trabajo vamos a analizar los que dedicó a dicho monarca el

  8. DOCLASP - Docking ligands to target proteins using spatial and electrostatic congruence extracted from a known holoenzyme and applying simple geometrical transformations.

    Science.gov (United States)

    Chakraborty, Sandeep

    2014-01-01

    The ability to accurately and effectively predict the interaction between proteins and small drug-like compounds has long intrigued researchers for pedagogic, humanitarian and economic reasons. Protein docking methods (AutoDock, GOLD, DOCK, FlexX and Glide to name a few) rank a large number of possible conformations of protein-ligand complexes using fast algorithms. Previously, it has been shown that structural congruence leading to the same enzymatic function necessitates the congruence of electrostatic properties (CLASP). The current work presents a methodology for docking a ligand into a target protein, provided that there is at least one known holoenzyme with ligand bound - DOCLASP (Docking using CLASP). The contact points of the ligand in the holoenzyme defines a motif, which is used to query the target enzyme using CLASP. If there are significant matches, the holoenzyme and the target protein are superimposed based on congruent atoms. The same linear and rotational transformations are also applied to the ligand, thus creating a unified coordinate framework having the holoenzyme, the ligand and the target enzyme. In the current work, the dipeptidyl peptidase-IV inhibitor vildagliptin was docked to the PI-PLC structure complexed with myo-inositol using DOCLASP. Also, corroboration of the docking of phenylthiourea to the modelled structure of polyphenol oxidase (JrPPO1) from walnut is provided based on the subsequently solved structure of JrPPO1 (PDBid:5CE9). Analysis of the binding of the antitrypanosomial drug suramin to nine non-homologous proteins in the PDB database shows a diverse set of binding motifs, and multiple binding sites in the phospholipase A2-likeproteins from the Bothrops genus of pitvipers. The conformational changes in the suramin molecule on binding highlights the challenges in docking flexible ligands into an already 'plastic' binding site. Thus, DOCLASP presents a method for 'soft docking' ligands to proteins with low computational

  9. Diversity of Pol IV function is defined by mutations at the maize rmr7 locus.

    Directory of Open Access Journals (Sweden)

    Jennifer L Stonaker

    2009-11-01

    Full Text Available Mutations affecting the heritable maintenance of epigenetic states in maize identify multiple small RNA biogenesis factors including NRPD1, the largest subunit of the presumed maize Pol IV holoenzyme. Here we show that mutations defining the required to maintain repression7 locus identify a second RNA polymerase subunit related to Arabidopsis NRPD2a, the sole second largest subunit shared between Arabidopsis Pol IV and Pol V. A phylogenetic analysis shows that, in contrast to representative eudicots, grasses have retained duplicate loci capable of producing functional NRPD2-like proteins, which is indicative of increased RNA polymerase diversity in grasses relative to eudicots. Together with comparisons of rmr7 mutant plant phenotypes and their effects on the maintenance of epigenetic states with parallel analyses of NRPD1 defects, our results imply that maize utilizes multiple functional NRPD2-like proteins. Despite the observation that RMR7/NRPD2, like NRPD1, is required for the accumulation of most siRNAs, our data indicate that different Pol IV isoforms play distinct roles in the maintenance of meiotically-heritable epigenetic information in the grasses.

  10. Genomic binding profiles of functionally distinct RNA polymerase III transcription complexes in human cells.

    Science.gov (United States)

    Moqtaderi, Zarmik; Wang, Jie; Raha, Debasish; White, Robert J; Snyder, Michael; Weng, Zhiping; Struhl, Kevin

    2010-05-01

    Genome-wide occupancy profiles of five components of the RNA polymerase III (Pol III) machinery in human cells identified the expected tRNA and noncoding RNA targets and revealed many additional Pol III-associated loci, mostly near short interspersed elements (SINEs). Several genes are targets of an alternative transcription factor IIIB (TFIIIB) containing Brf2 instead of Brf1 and have extremely low levels of TFIIIC. Strikingly, expressed Pol III genes, unlike nonexpressed Pol III genes, are situated in regions with a pattern of histone modifications associated with functional Pol II promoters. TFIIIC alone associates with numerous ETC loci, via the B box or a novel motif. ETCs are often near CTCF binding sites, suggesting a potential role in chromosome organization. Our results suggest that human Pol III complexes associate preferentially with regions near functional Pol II promoters and that TFIIIC-mediated recruitment of TFIIIB is regulated in a locus-specific manner.

  11. Characterization of enzymatic properties of human ribonucleotide reductase holoenzyme reconstituted in vitro from hRRM1, hRRM2, and p53R2 subunits.

    Science.gov (United States)

    Qiu, Weihua; Zhou, Bingsen; Darwish, Dana; Shao, Jimin; Yen, Yun

    2006-02-10

    Ribonucleotide reductase (RR) is a highly regulated enzyme in the deoxyribonucleotide synthesis pathway. RR is responsible for the de novo conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates, which are essential for DNA synthesis and repair. Besides two subunits, hRRM1 and hRRM2, p53R2 is a newly identified member of RR family that is induced by ultraviolet light in a p53-dependent manner. To understand the molecular interaction of RR subunits, we employed a eukaryotic expression system to express and purify all three subunits. After in vitro reconstitution, the results of [(3)H]CDP reduction assay showed that both eukaryotic recombinant hRRM2 and p53R2 proteins could interact with hRRM1 to form functional RR holoenzyme. The reconstituted RR activity was time-dependent and the reaction rate reached the plateau phase after 40min incubation. No matter the concentration, RR holoenzyme reconstituted from p53R2 and hRRM1 could only achieve about 40-75% kinetic activity of that from hRRM2 and hRRM1. The synthetic C-terminal heptapeptide competition assays confirmed that hRRM2 and p53R2 share the same binding site on hRRM1, but the binding site on hRRM1 demonstrated higher affinity for hRRM2 than for p53R2. In allosteric regulation assay, the effect of activation or inhibition of hRRM1 with ATP or dATP suggested that these effectors could regulate RR activity independent of different RR small subunits. Taken together, the eukaryotic expression system RR holoenzyme will provide a very useful tool to understand the molecular mechanisms of RR activity and the interactions of its subunits.

  12. Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.

    Science.gov (United States)

    Dergai, Oleksandr; Cousin, Pascal; Gouge, Jerome; Satia, Karishma; Praz, Viviane; Kuhlman, Tracy; Lhôte, Philippe; Vannini, Alessandro; Hernandez, Nouria

    2018-05-01

    RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters. © 2018 Dergai et al.; Published by Cold Spring Harbor Laboratory Press.

  13. Rbs1, a new protein implicated in RNA polymerase III biogenesis in yeast Saccharomyces cerevisiae.

    Science.gov (United States)

    Cieśla, Małgorzata; Makała, Ewa; Płonka, Marta; Bazan, Rafał; Gewartowski, Kamil; Dziembowski, Andrzej; Boguta, Magdalena

    2015-04-01

    Little is known about the RNA polymerase III (Pol III) complex assembly and its transport to the nucleus. We demonstrate that a missense cold-sensitive mutation, rpc128-1007, in the sequence encoding the C-terminal part of the second largest Pol III subunit, C128, affects the assembly and stability of the enzyme. The cellular levels and nuclear concentration of selected Pol III subunits were decreased in rpc128-1007 cells, and the association between Pol III subunits as evaluated by coimmunoprecipitation was also reduced. To identify the proteins involved in Pol III assembly, we performed a genetic screen for suppressors of the rpc128-1007 mutation and selected the Rbs1 gene, whose overexpression enhanced de novo tRNA transcription in rpc128-1007 cells, which correlated with increased stability, nuclear concentration, and interaction of Pol III subunits. The rpc128-1007 rbs1Δ double mutant shows a synthetic growth defect, indicating that rpc128-1007 and rbs1Δ function in parallel ways to negatively regulate Pol III assembly. Rbs1 physically interacts with a subset of Pol III subunits, AC19, AC40, and ABC27/Rpb5. Additionally, Rbs1 interacts with the Crm1 exportin and shuttles between the cytoplasm and nucleus. We postulate that Rbs1 binds to the Pol III complex or subcomplex and facilitates its translocation to the nucleus. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  14. Strand Displacement by DNA Polymerase III Occurs through a τ-ψ-χ Link to Single-stranded DNA-binding Protein Coating the Lagging Strand Template*

    OpenAIRE

    Yuan, Quan; McHenry, Charles S.

    2009-01-01

    In addition to the well characterized processive replication reaction catalyzed by the DNA polymerase III holoenzyme on single-stranded DNA templates, the enzyme possesses an intrinsic strand displacement activity on flapped templates. The strand displacement activity is distinguished from the single-stranded DNA-templated reaction by a high dependence upon single-stranded DNA binding protein and an inability of γ-complex to support the reaction in the absence of τ. However, if γ-complex is p...

  15. redD and actII-ORF4, Pathway-Specific Regulatory Genes for Antibiotic Production in Streptomyces coelicolor A3(2), Are Transcribed In Vitro by an RNA Polymerase Holoenzyme Containing σhrdD

    NARCIS (Netherlands)

    Fujii, T.; Gramajo, H.C.; Takano, E.; Bibb, M.J.

    1996-01-01

    redD and actII-ORF4, regulatory genes required for synthesis of the antibiotics undecylprodigiosin and actinorhodin by Streptomyces coelicolor A3(2), were transcribed in vitro by an RNA polymerase holoenzyme containing σhrdD. Disruption of hrdD had no effect on antibiotic production, indicating that

  16. Structural organizations of yeast RNase P and RNase MRP holoenzymes as revealed by UV-crosslinking studies of RNA–protein interactions

    Science.gov (United States)

    Khanova, Elena; Esakova, Olga; Perederina, Anna; Berezin, Igor; Krasilnikov, Andrey S.

    2012-01-01

    Eukaryotic ribonuclease (RNase) P and RNase MRP are closely related ribonucleoprotein complexes involved in the metabolism of various RNA molecules including tRNA, rRNA, and some mRNAs. While evolutionarily related to bacterial RNase P, eukaryotic enzymes of the RNase P/MRP family are much more complex. Saccharomyces cerevisiae RNase P consists of a catalytic RNA component and nine essential proteins; yeast RNase MRP has an RNA component resembling that in RNase P and 10 essential proteins, most of which are shared with RNase P. The structural organizations of eukaryotic RNases P/MRP are not clear. Here we present the results of RNA–protein UV crosslinking studies performed on RNase P and RNase MRP holoenzymes isolated from yeast. The results indicate locations of specific protein-binding sites in the RNA components of RNase P and RNase MRP and shed light on the structural organizations of these large ribonucleoprotein complexes. PMID:22332141

  17. Structural organizations of yeast RNase P and RNase MRP holoenzymes as revealed by UV-crosslinking studies of RNA-protein interactions.

    Science.gov (United States)

    Khanova, Elena; Esakova, Olga; Perederina, Anna; Berezin, Igor; Krasilnikov, Andrey S

    2012-04-01

    Eukaryotic ribonuclease (RNase) P and RNase MRP are closely related ribonucleoprotein complexes involved in the metabolism of various RNA molecules including tRNA, rRNA, and some mRNAs. While evolutionarily related to bacterial RNase P, eukaryotic enzymes of the RNase P/MRP family are much more complex. Saccharomyces cerevisiae RNase P consists of a catalytic RNA component and nine essential proteins; yeast RNase MRP has an RNA component resembling that in RNase P and 10 essential proteins, most of which are shared with RNase P. The structural organizations of eukaryotic RNases P/MRP are not clear. Here we present the results of RNA-protein UV crosslinking studies performed on RNase P and RNase MRP holoenzymes isolated from yeast. The results indicate locations of specific protein-binding sites in the RNA components of RNase P and RNase MRP and shed light on the structural organizations of these large ribonucleoprotein complexes.

  18. FACT facilitates chromatin transcription by RNA polymerases I and III

    DEFF Research Database (Denmark)

    Birch, Joanna L; Tan, Bertrand C-M; Panov, Kostya I

    2009-01-01

    Efficient transcription elongation from a chromatin template requires RNA polymerases (Pols) to negotiate nucleosomes. Our biochemical analyses demonstrate that RNA Pol I can transcribe through nucleosome templates and that this requires structural rearrangement of the nucleosomal core particle....... The subunits of the histone chaperone FACT (facilitates chromatin transcription), SSRP1 and Spt16, co-purify and co-immunoprecipitate with mammalian Pol I complexes. In cells, SSRP1 is detectable at the rRNA gene repeats. Crucially, siRNA-mediated repression of FACT subunit expression in cells results...... in a significant reduction in 47S pre-rRNA levels, whereas synthesis of the first 40 nt of the rRNA is not affected, implying that FACT is important for Pol I transcription elongation through chromatin. FACT also associates with RNA Pol III complexes, is present at the chromatin of genes transcribed by Pol III...

  19. RNA polymerase III transcription - regulated by chromatin structure and regulator of nuclear chromatin organization.

    Science.gov (United States)

    Pascali, Chiara; Teichmann, Martin

    2013-01-01

    RNA polymerase III (Pol III) transcription is regulated by modifications of the chromatin. DNA methylation and post-translational modifications of histones, such as acetylation, phosphorylation and methylation have been linked to Pol III transcriptional activity. In addition to being regulated by modifications of DNA and histones, Pol III genes and its transcription factors have been implicated in the organization of nuclear chromatin in several organisms. In yeast, the ability of the Pol III transcription system to contribute to nuclear organization seems to be dependent on direct interactions of Pol III genes and/or its transcription factors TFIIIC and TFIIIB with the structural maintenance of chromatin (SMC) protein-containing complexes cohesin and condensin. In human cells, Pol III genes and transcription factors have also been shown to colocalize with cohesin and the transcription regulator and genome organizer CCCTC-binding factor (CTCF). Furthermore, chromosomal sites have been identified in yeast and humans that are bound by partial Pol III machineries (extra TFIIIC sites - ETC; chromosome organizing clamps - COC). These ETCs/COC as well as Pol III genes possess the ability to act as boundary elements that restrict spreading of heterochromatin.

  20. Nucleosome Positioning and NDR Structure at RNA Polymerase III Promoters

    DEFF Research Database (Denmark)

    Helbo, Alexandra Søgaard; Lay, Fides D; Jones, Peter A

    2017-01-01

    Chromatin is structurally involved in the transcriptional regulation of all genes. While the nucleosome positioning at RNA polymerase II (pol II) promoters has been extensively studied, less is known about the chromatin structure at pol III promoters in human cells. We use a high...

  1. Solution Structure of Pfu RPP21, a Component of the Archaeal RNase P Holoenzyme, and Interactions with its RPP29 Protein Partner

    Science.gov (United States)

    Amero, Carlos D; Boomershine, William P; Xu, Yiren; Foster, Mark

    2009-01-01

    RNase P is the ubiquitous ribonucleoprotein metalloenzyme responsible for cleaving the 5′-leader sequence of precursor tRNAs during their maturation. While the RNA subunit is catalytically active on its own at high monovalent and divalent ion concentration, four proteins subunits are associated with archaeal RNase P activity in vivo: RPP21, RPP29, RPP30 and POP5. These proteins have been shown to function in pairs: RPP21-RPP29 and POP5-RPP30. We have determined the solution structure of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus (Pfu) using conventional and paramagnetic NMR techniques. Pfu RPP21 in solution consists of an unstructured N-terminus, two alpha helices, a zinc binding motif, and an unstructured C-terminus. Moreover, we have used chemical shift perturbations to characterize the interaction of RPP21 with Pfu RPP29. The data show that the primary contact with RPP29 is localized to the two helices of RPP21. This information represents a fundamental step towards understanding structure-function relationships of the archaeal RNase P holoenzyme. PMID:18922021

  2. Architecture of the nitric-oxide synthase holoenzyme reveals large conformational changes and a calmodulin-driven release of the FMN domain.

    Science.gov (United States)

    Yokom, Adam L; Morishima, Yoshihiro; Lau, Miranda; Su, Min; Glukhova, Alisa; Osawa, Yoichi; Southworth, Daniel R

    2014-06-13

    Nitric-oxide synthase (NOS) is required in mammals to generate NO for regulating blood pressure, synaptic response, and immune defense. NOS is a large homodimer with well characterized reductase and oxygenase domains that coordinate a multistep, interdomain electron transfer mechanism to oxidize l-arginine and generate NO. Ca(2+)-calmodulin (CaM) binds between the reductase and oxygenase domains to activate NO synthesis. Although NOS has long been proposed to adopt distinct conformations that alternate between interflavin and FMN-heme electron transfer steps, structures of the holoenzyme have remained elusive and the CaM-bound arrangement is unknown. Here we have applied single particle electron microscopy (EM) methods to characterize the full-length of the neuronal isoform (nNOS) complex and determine the structural mechanism of CaM activation. We have identified that nNOS adopts an ensemble of open and closed conformational states and that CaM binding induces a dramatic rearrangement of the reductase domain. Our three-dimensional reconstruction of the intact nNOS-CaM complex reveals a closed conformation and a cross-monomer arrangement with the FMN domain rotated away from the NADPH-FAD center, toward the oxygenase dimer. This work captures, for the first time, the reductase-oxygenase structural arrangement and the CaM-dependent release of the FMN domain that coordinates to drive electron transfer across the domains during catalysis. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  3. Genetics Home Reference: Pol III-related leukodystrophy

    Science.gov (United States)

    ... 2011 Sep 9;89(3):415-23. doi: 10.1016/j.ajhg.2011.07.014. Erratum in: Am ... Jan 15;300(1-2):179-81. doi: 10.1016/j.jns.2010.09.009. Epub 2010 Sep ... Sep 15;320(1-2):102-5. doi: 10.1016/j.jns.2012.07.005. Epub 2012 Jul ...

  4. Regulation of RNA polymerase III transcription during transformation of human IMR90 fibroblasts with defined genetic elements.

    Science.gov (United States)

    Durrieu-Gaillard, Stéphanie; Dumay-Odelot, Hélène; Boldina, Galina; Tourasse, Nicolas J; Allard, Delphine; André, Fabrice; Macari, Françoise; Choquet, Armelle; Lagarde, Pauline; Drutel, Guillaume; Leste-Lasserre, Thierry; Petitet, Marion; Lesluyes, Tom; Lartigue-Faustin, Lydia; Dupuy, Jean-William; Chibon, Frédéric; Roeder, Robert G; Joubert, Dominique; Vagner, Stéphan; Teichmann, Martin

    2018-01-01

    RNA polymerase (Pol) III transcribes small untranslated RNAs that are essential for cellular homeostasis and growth. Its activity is regulated by inactivation of tumor suppressor proteins and overexpression of the oncogene c-MYC, but the concerted action of these tumor-promoting factors on Pol III transcription has not yet been assessed. In order to comprehensively analyse the regulation of Pol III transcription during tumorigenesis we employ a model system that relies on the expression of five genetic elements to achieve cellular transformation. Expression of these elements in six distinct transformation intermediate cell lines leads to the inactivation of TP53, RB1, and protein phosphatase 2A, as well as the activation of RAS and the protection of telomeres by TERT, thereby conducting to full tumoral transformation of IMR90 fibroblasts. Transformation is accompanied by moderately enhanced levels of a subset of Pol III-transcribed RNAs (7SK; MRP; H1). In addition, mRNA and/or protein levels of several Pol III subunits and transcription factors are upregulated, including increased protein levels of TFIIIB and TFIIIC subunits, of SNAPC1 and of Pol III subunits. Strikingly, the expression of POLR3G and of SNAPC1 is strongly enhanced during transformation in this cellular transformation model. Collectively, our data indicate that increased expression of several components of the Pol III transcription system accompanied by a 2-fold increase in steady state levels of a subset of Pol III RNAs is sufficient for sustaining tumor formation.

  5. Richard III

    DEFF Research Database (Denmark)

    Lauridsen, Palle Schantz

    2017-01-01

    Kort analyse af Shakespeares Richard III med fokus på, hvordan denne skurk fremstilles, så tilskuere (og læsere) langt henad vejen kan føle sympati med ham. Med paralleller til Netflix-serien "House of Cards"......Kort analyse af Shakespeares Richard III med fokus på, hvordan denne skurk fremstilles, så tilskuere (og læsere) langt henad vejen kan føle sympati med ham. Med paralleller til Netflix-serien "House of Cards"...

  6. PARDISEKO III

    International Nuclear Information System (INIS)

    Jordan, H.; Sack, C.

    1975-05-01

    This report gives a detailed description of the latest version of the PARDISEKO code, PARDISEKO III, with particular emphasis on the numerical and programming methods employed. The physical model and its relation to nuclear safety as well as a description and the results of confirming experiments are treated in detail in the Karlsruhe Nuclear Research Centre report KFK-1989. (orig.) [de

  7. Unidad 4: Crear Polígonos

    OpenAIRE

    Gómez Trigueros, Isabel María

    2015-01-01

    En esta unidad aprenderéis a crear vuestros propios polígonos con Google Earth para delimitar un área geográfica. Podréis modificar el tamaño de los polígonos, el color del área y su perímetro, incluso elaborar polígonos en 3D para superponer distintas variables sobre un paisaje concreto.

  8. Observatorio de Política Internacional I. COLOMBIA - Una paz esquiva

    OpenAIRE

    Neira Fernández, Enrique

    2007-01-01

    Observatorio de Política Internacional I. COLOMBIA (Neira Fernández, Enrique) RECIENTES Año 2008 Colombia. Cerca el fin del paramilitarismo Colombia. Cerca el fin de las Farc Colombia. Conflicto armado y desarrollo humano Colombia. Política exterior Colombia. Inicia el 2008 con buen pie Año 2007 Colombia. Tras las pasadas elecciones Colombia. El espectro de las próximas elecciones Colombia. La garrocha de Uribe Colombia. III Parapolíticos Colombia. II...

  9. Observatorio de Política Internacional I. COLOMBIA - Una nueva estrategia de paz

    OpenAIRE

    Neira Fernández, Enrique

    2007-01-01

    Observatorio de Política Internacional I. COLOMBIA (Neira Fernández, Enrique) RECIENTES Año 2008 Colombia. Cerca el fin del paramilitarismo Colombia. Cerca el fin de las Farc Colombia. Conflicto armado y desarrollo humano Colombia. Política exterior Colombia. Inicia el 2008 con buen pie Año 2007 Colombia. Tras las pasadas elecciones Colombia. El espectro de las próximas elecciones Colombia. La garrocha de Uribe Colombia. III Parapolíticos Colombia. II...

  10. Observatorio de Política Internacional I. COLOMBIA - Una paz secuestrada

    OpenAIRE

    Neira Fernández, Enrique

    2007-01-01

    Observatorio de Política Internacional I. COLOMBIA (Neira Fernández, Enrique) RECIENTES Año 2008 Colombia. Cerca el fin del paramilitarismo Colombia. Cerca el fin de las Farc Colombia. Conflicto armado y desarrollo humano Colombia. Política exterior Colombia. Inicia el 2008 con buen pie Año 2007 Colombia. Tras las pasadas elecciones Colombia. El espectro de las próximas elecciones Colombia. La garrocha de Uribe Colombia. III Parapolíticos Colombia. II...

  11. Observatorio de Política Internacional I. COLOMBIA - Los momentos de la paz

    OpenAIRE

    Neira Fernández, Enrique

    2012-01-01

    Observatorio de Política Internacional I. COLOMBIA (Neira Fernández, Enrique) RECIENTES Año 2008 Colombia. Cerca el fin del paramilitarismo Colombia. Cerca el fin de las Farc Colombia. Conflicto armado y desarrollo humano Colombia. Política exterior Colombia. Inicia el 2008 con buen pie Año 2007 Colombia. Tras las pasadas elecciones Colombia. El espectro de las próximas elecciones Colombia. La garrocha de Uribe Colombia. III Parapolíticos Colombia. II...

  12. Observatorio de Política Internacional I. COLOMBIA - Siempre es tarde para la paz

    OpenAIRE

    Neira Fernández, Enrique

    2007-01-01

    Observatorio de Política Internacional I. COLOMBIA (Neira Fernández, Enrique) RECIENTES Año 2008 Colombia. Cerca el fin del paramilitarismo Colombia. Cerca el fin de las Farc Colombia. Conflicto armado y desarrollo humano Colombia. Política exterior Colombia. Inicia el 2008 con buen pie Año 2007 Colombia. Tras las pasadas elecciones Colombia. El espectro de las próximas elecciones Colombia. La garrocha de Uribe Colombia. III Parapolíticos Colombia. II...

  13. Observatorio de Política Internacional I. COLOMBIA - Viraje en el proceso de paz

    OpenAIRE

    Neira Fernández, Enrique

    2007-01-01

    Observatorio de Política Internacional I. COLOMBIA (Neira Fernández, Enrique) RECIENTES Año 2008 Colombia. Cerca el fin del paramilitarismo Colombia. Cerca el fin de las Farc Colombia. Conflicto armado y desarrollo humano Colombia. Política exterior Colombia. Inicia el 2008 con buen pie Año 2007 Colombia. Tras las pasadas elecciones Colombia. El espectro de las próximas elecciones Colombia. La garrocha de Uribe Colombia. III Parapolíticos Colombia. II...

  14. Fermilab III

    International Nuclear Information System (INIS)

    Anon.

    1990-01-01

    The total ongoing plans for Fermilab are wrapped up in the Fermilab III scheme, centrepiece of which is the proposal for a new Main Injector. The Laboratory has been awarded a $200,000 Illinois grant which will be used to initiate environmental assessment and engineering design of the Main Injector, while a state review panel recommended that the project should also benefit from $2 million of funding

  15. Fermilab III

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1990-09-15

    The total ongoing plans for Fermilab are wrapped up in the Fermilab III scheme, centrepiece of which is the proposal for a new Main Injector. The Laboratory has been awarded a $200,000 Illinois grant which will be used to initiate environmental assessment and engineering design of the Main Injector, while a state review panel recommended that the project should also benefit from $2 million of funding.

  16. Sangres políticas

    Directory of Open Access Journals (Sweden)

    Gabriel Gatti

    2018-03-01

    Full Text Available Trabajamos en las tensiones entre dos continentes en permanente disputa: “sangre” y “política”, “realidad” y “dispositivo”, “naturaleza” y “cultura”. Son viejos asuntos, y viejas tensiones, pero que no dejan de actualizarse y que ahora se manifiestan por doquier en cuestiones como la biometría, los mapas genéticos, la identificación de desaparecidos, las políticas de la identidad indígena o de género o de menores o de drogas, la gestación subrogada o la gestión de la marginalidad. Los diez textos recogidos en este número especial discuten desde una mirada interdisciplinaria sobre la presencia de la sangre —en sus distintas declinaciones— en la definición contemporánea de lo que entendemos por identidad, derechos humanos o ciudadanía.

  17. Política, marketing e neoliberalismo

    Directory of Open Access Journals (Sweden)

    Pedro Roberto Ferreira

    2004-07-01

    Full Text Available O texto contém uma análise da relação política e sua possível consciência no seu momento específico compreendido pelo processo eleitoral (Londrina - eleições 92. Como este processo eleitoral em nossos dias, nutre-se de algumas técnicas conhecidas como Marketing Político-Eleitoral, e de como estas, contribuem para uma dinâmica de esvaziamento da vida política propriamente dita. Procura ainda, conectar esse esvaziamento político e suas consequências, com o avanço da concepção neoliberal cujo ápice parece fazer coincidir a política como mais uma manifestação de Mercado.

  18. Retrotransposons. An RNA polymerase III subunit determines sites of retrotransposon integration.

    Science.gov (United States)

    Bridier-Nahmias, Antoine; Tchalikian-Cosson, Aurélie; Baller, Joshua A; Menouni, Rachid; Fayol, Hélène; Flores, Amando; Saïb, Ali; Werner, Michel; Voytas, Daniel F; Lesage, Pascale

    2015-05-01

    Mobile genetic elements are ubiquitous. Their integration site influences genome stability and gene expression. The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae integrates upstream of RNA polymerase III (Pol III)-transcribed genes, yet the primary determinant of target specificity has remained elusive. Here we describe an interaction between Ty1 integrase and the AC40 subunit of Pol III and demonstrate that AC40 is the predominant determinant targeting Ty1 integration upstream of Pol III-transcribed genes. Lack of an integrase-AC40 interaction dramatically alters target site choice, leading to a redistribution of Ty1 insertions in the genome, mainly to chromosome ends. The mechanism of target specificity allows Ty1 to proliferate and yet minimizes genetic damage to its host. Copyright © 2015, American Association for the Advancement of Science.

  19. Edad y cultura política

    Directory of Open Access Journals (Sweden)

    MANUEL JUSTEL

    1992-01-01

    Full Text Available En este artículo se analiza la evolución de algunos aspectos de la cultura política y democrática durante los años ochenta: información y competencia política, participación política, actitudes hacia los partidos y orientaciones políticas. Los datos proceden de sendas encuestas realizadas en 1980 y 1989. Mediante análisis de cohortes de edad, controlando sexo y nivel de estudios, se comprueba el grado diferencial de expansión de actitudes democráticas en los grupos de edad, controlando sexo y nivel de estudios, se comprueba el grado diferencial de expansión de actitudes democráticas en los grupos de edad, el efecto homogeneizador que conlleva y el influjo estratégico de la educación. En el marco teórico de la socialización política en edad adulta y de las relaciones entre sistema social y sistema político, se interpretan los cambios de actitudes distinguiendo efectos de ciclo vital, de cohorte y de periodo. Al mismo tiempo, se discuten algunas consecuencias políticas del envejecimiento poblacional y se constata de un grado notable de plasticidad actitudinal y de adhesión creciente a la democracia de las cohortes de edad avanzada.

  20. Fuels and Petroleum, Oil & Lubricants (POL) Laboratories

    Data.gov (United States)

    Federal Laboratory Consortium — The Fuels and Lubricants Technology Team operates and maintains the Fuels and POL Labs at TARDEC. Lab experts adhere to standardized American Society for Testing and...

  1. Polyadenylation of RNA transcribed from mammalian SINEs by RNA polymerase III: Complex requirements for nucleotide sequences.

    Science.gov (United States)

    Borodulina, Olga R; Golubchikova, Julia S; Ustyantsev, Ilia G; Kramerov, Dmitri A

    2016-02-01

    It is generally accepted that only transcripts synthesized by RNA polymerase II (e.g., mRNA) were subject to AAUAAA-dependent polyadenylation. However, we previously showed that RNA transcribed by RNA polymerase III (pol III) from mouse B2 SINE could be polyadenylated in an AAUAAA-dependent manner. Many species of mammalian SINEs end with the pol III transcriptional terminator (TTTTT) and contain hexamers AATAAA in their A-rich tail. Such SINEs were united into Class T(+), whereas SINEs lacking the terminator and AATAAA sequences were classified as T(-). Here we studied the structural features of SINE pol III transcripts that are necessary for their polyadenylation. Eight and six SINE families from classes T(+) and T(-), respectively, were analyzed. The replacement of AATAAA with AACAAA in T(+) SINEs abolished the RNA polyadenylation. Interestingly, insertion of the polyadenylation signal (AATAAA) and pol III transcription terminator in T(-) SINEs did not result in polyadenylation. The detailed analysis of three T(+) SINEs (B2, DIP, and VES) revealed areas important for the polyadenylation of their pol III transcripts: the polyadenylation signal and terminator in A-rich tail, β region positioned immediately downstream of the box B of pol III promoter, and τ region located upstream of the tail. In DIP and VES (but not in B2), the τ region is a polypyrimidine motif which is also characteristic of many other T(+) SINEs. Most likely, SINEs of different mammals acquired these structural features independently as a result of parallel evolution. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III-transcribed genes in budding yeast.

    Science.gov (United States)

    Belagal, Praveen; Normand, Christophe; Shukla, Ashutosh; Wang, Renjie; Léger-Silvestre, Isabelle; Dez, Christophe; Bhargava, Purnima; Gadal, Olivier

    2016-10-15

    The association of RNA polymerase III (Pol III)-transcribed genes with nucleoli seems to be an evolutionarily conserved property of the spatial organization of eukaryotic genomes. However, recent studies of global chromosome architecture in budding yeast have challenged this view. We used live-cell imaging to determine the intranuclear positions of 13 Pol III-transcribed genes. The frequency of association with nucleolus and nuclear periphery depends on linear genomic distance from the tethering elements-centromeres or telomeres. Releasing the hold of the tethering elements by inactivating centromere attachment to the spindle pole body or changing the position of ribosomal DNA arrays resulted in the association of Pol III-transcribed genes with nucleoli. Conversely, ectopic insertion of a Pol III-transcribed gene in the vicinity of a centromere prevented its association with nucleolus. Pol III-dependent transcription was independent of the intranuclear position of the gene, but the nucleolar recruitment of Pol III-transcribed genes required active transcription. We conclude that the association of Pol III-transcribed genes with the nucleolus, when permitted by global chromosome architecture, provides nucleolar and/or nuclear peripheral anchoring points contributing locally to intranuclear chromosome organization. © 2016 Belagal et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  3. La carrera política y el capital político

    Directory of Open Access Journals (Sweden)

    Manuel Alcántara-Sáez

    2017-01-01

    Full Text Available Se trata de una propuesta de naturaleza teórica para el estudio de las carreras políticas desde la perspectiva de la existencia de tres momentos diferentes (entrada, desempeño y salida, en conformidad con el uso del capital político que gestionan los políticos. Se abordan teóricamente distintos patrones de capital político así como su impacto sobre las trayectorias políticas seguidas. El peso del tiempo transcurrido y los ingresos recibidos por la actividad política son igualmente considerados. Político es aquella persona que es elegida en un proceso electoral y/o que es nominada en un puesto de confianza por alguien elegido, también lo es quien tiene un cargo orgánico en una institución como un partido político. A ello debe sumarse recibir una remuneración por esa actividad.

  4. Observatorio de Política Internacional I. COLOMBIA - Colombia. Crónica de un proceso de paz fracasado

    OpenAIRE

    Neira Fernández, Enrique

    2007-01-01

    Observatorio de Política Internacional I. COLOMBIA (Neira Fernández, Enrique) RECIENTES Año 2008 Colombia. Cerca el fin del paramilitarismo Colombia. Cerca el fin de las Farc Colombia. Conflicto armado y desarrollo humano Colombia. Política exterior Colombia. Inicia el 2008 con buen pie Año 2007 Colombia. Tras las pasadas elecciones Colombia. El espectro de las próximas elecciones Colombia. La garrocha de Uribe Colombia. III Parapolíticos Colombia. II...

  5. Observatorio de Política Internacional I. COLOMBIA - El proceso de paz en sala de emergencia

    OpenAIRE

    Neira Fernández, Enrique

    2007-01-01

    Observatorio de Política Internacional I. COLOMBIA (Neira Fernández, Enrique) RECIENTES Año 2008 Colombia. Cerca el fin del paramilitarismo Colombia. Cerca el fin de las Farc Colombia. Conflicto armado y desarrollo humano Colombia. Política exterior Colombia. Inicia el 2008 con buen pie Año 2007 Colombia. Tras las pasadas elecciones Colombia. El espectro de las próximas elecciones Colombia. La garrocha de Uribe Colombia. III Parapolíticos Colombia. II...

  6. Involvement of specialized DNA polymerases Pol II, Pol IV and DnaE2 in DNA replication in the absence of Pol I in Pseudomonas putida

    International Nuclear Information System (INIS)

    Sidorenko, Julia; Jatsenko, Tatjana; Saumaa, Signe; Teras, Riho; Tark-Dame, Mariliis; Horak, Rita; Kivisaar, Maia

    2011-01-01

    The majority of bacteria possess a different set of specialized DNA polymerases than those identified in the most common model organism Escherichia coli. Here, we have studied the ability of specialized DNA polymerases to substitute Pol I in DNA replication in Pseudomonas putida. Our results revealed that P. putida Pol I-deficient cells have severe growth defects in LB medium, which is accompanied by filamentous cell morphology. However, growth of Pol I-deficient bacteria on solid rich medium can be restored by reduction of reactive oxygen species in cells. Also, mutants with improved growth emerge rapidly. Similarly to the initial Pol I-deficient P. putida, its adapted derivatives express a moderate mutator phenotype, which indicates that DNA replication carried out in the absence of Pol I is erroneous both in the original Pol I-deficient bacteria and the adapted derivatives. Analysis of the spectra of spontaneous Rif r mutations in P. putida strains lacking different DNA polymerases revealed that the presence of specialized DNA polymerases Pol II and Pol IV influences the frequency of certain base substitutions in Pol I-proficient and Pol I-deficient backgrounds in opposite ways. Involvement of another specialized DNA polymerase DnaE2 in DNA replication in Pol I-deficient bacteria is stimulated by UV irradiation of bacteria, implying that DnaE2-provided translesion synthesis partially substitutes the absence of Pol I in cells containing heavily damaged DNA.

  7. Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass.

    Science.gov (United States)

    Lee, Young-Sam; Gregory, Mark T; Yang, Wei

    2014-02-25

    DNA polymerase ζ (Pol ζ) is a eukaryotic B-family DNA polymerase that specializes in translesion synthesis and is essential for normal embryogenesis. At a minimum, Pol ζ consists of a catalytic subunit Rev3 and an accessory subunit Rev7. Mammalian Rev3 contains >3,000 residues and is twice as large as the yeast homolog. To date, no vertebrate Pol ζ has been purified for biochemical characterization. Here we report purification of a series of human Rev3 deletion constructs expressed in HEK293 cells and identification of a minimally catalytically active human Pol ζ variant. With a tagged form of an active Pol ζ variant, we isolated two additional accessory subunits of human Pol ζ, PolD2 and PolD3. The purified four-subunit Pol ζ4 (Rev3-Rev7-PolD2-PolD3) is much more efficient and more processive at bypassing a 1,2-intrastrand d(GpG)-cisplatin cross-link than the two-subunit Pol ζ2 (Rev3-Rev7). We show that complete bypass of cisplatin lesions requires Pol η to insert dCTP opposite the 3' guanine and Pol ζ4 to extend the primers.

  8. Agendamento de Políticas Públicas

    Directory of Open Access Journals (Sweden)

    Rosane Rosa

    2011-07-01

    Full Text Available Aborda-se o conceito de advocacy e contra-agendamento como uma forma de a Sociedade Civil incluir suas causas na mídia, objetivando a tematização e a possibilidade de transformar-se em política pública. Analisa-se a reportagem “Uma conquista longe das ruas”; resultado de um agendamento compartilhado e que, em muitos aspectos, serve de referência para cobertura de políticas públicas sociais.

  9. Characterization of Mycobacterium smegmatis PolD2 and PolD1 as RNA/DNA polymerases homologous to the POL domain of bacterial DNA ligase D.

    Science.gov (United States)

    Zhu, Hui; Bhattarai, Hitesh; Yan, Han-Guang; Shuman, Stewart; Glickman, Michael S

    2012-12-21

    Mycobacteria exploit nonhomologous end-joining (NHEJ) to repair DNA double-strand breaks. The core NHEJ machinery comprises the homodimeric DNA end-binding protein Ku and DNA ligase D (LigD), a modular enzyme composed of a C-terminal ATP-dependent ligase domain (LIG), a central 3'-phosphoesterase domain (PE), and an N-terminal polymerase domain (POL). LigD POL is proficient at adding templated and nontemplated deoxynucleotides and ribonucleotides to DNA ends in vitro and is the catalyst in vivo of unfaithful NHEJ events involving nontemplated single-nucleotide additions to blunt DSB ends. Here, we identify two mycobacterial proteins, PolD1 and PolD2, as stand-alone homologues of the LigD POL domain. Biochemical characterization of PolD1 and PolD2 shows that they resemble LigD POL in their monomeric quaternary structures, their ability to add templated and nontemplated nucleotides to primer-templates and blunt ends, and their preference for rNTPs versus dNTPs. Deletion of polD1, polD2, or both from a Mycobacterium smegmatis strain carrying an inactivating mutation in LigD POL failed to reveal a role for PolD1 or PolD2 in templated nucleotide additions during NHEJ of 5'-overhang DSBs or in clastogen resistance. Whereas our results document the existence and characteristics of new stand-alone members of the LigD POL family of RNA/DNA polymerases, they imply that other polymerases can perform fill-in synthesis during mycobacterial NHEJ.

  10. Ingeniero, político, economista, inventor

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    Jimena Montaña Cuéllar

    2001-01-01

    Full Text Available Técnica y utopía. Biografía intelectual y política de Alejandro López, 1876-1940. Alberto Mayor Mora. Fondo Editorial Universidad Eafit, colección Cielos de Arena, Medellín, 2001, 621 págs., il.

  11. El marketing político

    Directory of Open Access Journals (Sweden)

    Alvaro H. Cifuentes G.

    2015-04-01

    Full Text Available RESUMEN Si un candidato desea ser elegido, las técnicas de marketing aplicadas a la política, le son imprescindibles. El marketing político (Politing es el análisis  metódico de los diferentes segmentos que integran el ámbito político de una sociedad y de los factores que puedan modificarlos. Se aplican al candidato, a los votantes, al análisis de la publicidad política, a los simpatizantes, a desconocedores  del partido y a los indecisos. Los candidatos colombianos lo están aplicando, y compañías nuestras exportan servicios  de asesoría de países suramericanos.Promocionar  un candidato implica estructurar una compañía tal como una empresa, aplicando técnicas de ventas, de fijación de cuotas y de planeación estratégica.

  12. Blogs, artefactos y política

    Directory of Open Access Journals (Sweden)

    María Belén Albornoz

    2010-05-01

    Full Text Available Este artículo problematiza la acepción de lo público en el espacio virtual que surge en la blogosfera política ecuatoriana en el contexto de la Asamblea Nacional Constituyente. A partir del análisis de la extensión de la política a los lugares virtuales, se aborda la blogosfera como proyecto político y la tecnología como formas de ordenamiento del mundo que la sociedad estabiliza. Se considera a la blogosfera política un espacio público regulado con la capacidad de ordenar y clasificar el mundo virtual por medio de dispositivos sociales, políticos y tecnológicos. Finalmente se propone el estudio del nuevo espacio público virtual desde un enfoque tecno-social que dé cuenta del entorno virtual como espacio formador de conductas.This article problematizes the acceptance of the public in the virtual space emerging in the Ecuadorian political blogosphere, in the context of the National Constitutional Assembly. Beginning with an analysis of the spread of politics to virtual spaces, the blogosphere is approached as a political project, and technology as ways of ordering the world that society stabilizes. The political blogosphere is taken to be a regulated public space with the ability to order and classify the virtual world by means of social, political and technological tools. Finally, the study of this new virtual public space from a techno-social focus is proposed, taking into account virtual surroundings as a space in which conducts are formed.

  13. Políticas activas en las prestaciones por desempleo

    OpenAIRE

    Gil Jiménez, Javier

    2016-01-01

    El presente trabajo explica el tema de las políticas activas y políticas activas en las prestaciones por desempleo, tanto a nivel nacional como internacional. Para explicarlo se definen las políticas activas y sus funciones, tanto de formación, orientación y reinserción laboral. También se hace mención a las reformas llevadas a cabo en las diferentes políticas y el surgimiento de nuevos programas como función de políticas activas encaminadas a la reinserción laboral. Departamento de Derech...

  14. Antithrombin III blood test

    Science.gov (United States)

    ... medlineplus.gov/ency/article/003661.htm Antithrombin III blood test To use the sharing features on this page, ... a protein that helps control blood clotting. A blood test can determine the amount of AT III present ...

  15. Polímeros inteligentes. Informe de vigilancia tecnológica

    OpenAIRE

    Cano Serrano, Encarnación; Urbina Fraile, Marina

    2008-01-01

    Este informe de Vigilancia Tecnológica ha sido cofinanciado con Fondos FEDER y se ha realizado dentro del marco del Contrato Programa suscrito entre la Dirección General de Universidades e Investigación de la Comunidad de Madrid y la Universidad Carlos III de Madrid, con la colaboración del Parque Científico de Leganés que gestiona el Círculo de Innovación en Materiales, Tecnología Aeroespacial y Nanotecnología. Los sistemas poliméricos inteligentes o polímeros sensibles al estímulo son...

  16. Amor y política.

    OpenAIRE

    Hernando Bernal.

    1998-01-01

    Este trabajo pretende hacer una serie de consideraciones que apuntan sobre todo a extraer una definición de lo que es «la política» para el psicoanálisis a partir de una observación que hace Jacques Alain Miller en su texto Lógicas de la vida amorosa sobre cómo Freud introduce una «teoría política» a partir de su texto Psicología de las masas y análisis del yo.En dicho texto Freud hace ver el poder ordenador y apaciguador del amor) significante amo) en la medida en que una masa no es más que ...

  17. Hans Kelsen: pensador político

    Directory of Open Access Journals (Sweden)

    Sara Lagi

    2011-01-01

    Full Text Available Hasta la publicación de Sobre la esencia y el valor de la democracia, Hans Kelsen era solo conocido como experto en derecho público. El valor de este artículo de Lagi radica en rescatar de la obra kelseniana un aspecto casi por completo olvidado por la crítica, a saber, sus análisis sobre el significado y las características de la democracia parlamentaria en los Estados modernos. Se aborda la cuestión no solo desde el debate teórico sino también desde su contexto histórico¿político. La teoría política de Kelsen es considerada una parte integral de su doctrina central, presentada en Teoría pura del derecho, su obra más conspicua. Un análisis del trabajo sobre la esencia y el valor de la democracia nos restituye la imagen de un Kelsen como original pensador político. Se analizan las dos ediciones de Sobre la esencia y el valor de la democracia (1920¿1929 con estos propósitos: comprender por qué decidió dedicarse a la teoría de la democracia un teórico que rigurosamente defendió la separación de la esfera jurídica respecto de la historia, la filosofía y la política; y por qué no se limitó a explicar la esencia de la democracia sino que decidió concentrarse en clarificar qué se entiende por valor de la democracia.

  18. Las rentas de las exportaciones y la política comercial con oligopolio

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    Marcos Ávalos

    2005-01-01

    Full Text Available Para una industria oligopólica, este ensayo analiza la manera cómo la políti -ca comercial puede depender de la naturaleza competitiva de los mercados.Estudiamos los efectos de las fusiones nacionales y extranjeras en la políticaco mer cial óp ti ma del país de re fe ren cia y en las ren tas de las ex por ta cio nes yel bienestar interno. Hay cuatro resultados principales: i se de mues tra quesi el país de referencia aplica su política comercial óptima, siempre perderáa resultas de una fusión extranje ra; ii cuan do el país de re fe ren cia apli ca supolítica comercial óptima, una fusión extranjera disminuirá las rentas de lasexportaciones; iii cuando el gobierno responde óptimamente a una fusióninterna, ésta no afectará el bienestar nacional, y iv cuan do el go bier no res -pon de óp ti ma men te a una fu sión in ter na, ésta no afec ta rá las ren tas de lasexportaciones.

  19. Elecciones 2003: spots políticos y cultura política

    OpenAIRE

    Concepción Virriel

    2004-01-01

    El presente trabajo aborda el tema de las elecciones federales de 2003, en especial el fenómeno del abstencionismo. Para ello se vincula el comportamiento electoral en dichas elecciones con la cultura política, en donde los aspectos básicos son la cultura del fraude heredada de los gobiernos priístas y la redefinición de los partidos políticos ante una nueva coyuntura, y cómo éstos aspectos influyeron en la forma de comunicarse mediante sus spots. El análisis de dichos spots comprende el anál...

  20. Prototype Theory Based Feature Representation for PolSAR Images

    OpenAIRE

    Huang Xiaojing; Yang Xiangli; Huang Pingping; Yang Wen

    2016-01-01

    This study presents a new feature representation approach for Polarimetric Synthetic Aperture Radar (PolSAR) image based on prototype theory. First, multiple prototype sets are generated using prototype theory. Then, regularized logistic regression is used to predict similarities between a test sample and each prototype set. Finally, the PolSAR image feature representation is obtained by ensemble projection. Experimental results of an unsupervised classification of PolSAR images show that our...

  1. As armadilhas do tripé da política macroeconômica brasileira

    Directory of Open Access Journals (Sweden)

    ANDRÉ NASSIF

    2015-09-01

    Full Text Available RESUMOEste artigo analisa o chamado tripé da política macroeconômica brasileira, que desde 1999 tem combinado um regime de metas de inflação, um regime de taxa de câmbio flutuante e metas de superávit fiscal primário. A menos que o seu modus operandi seja alterado, o tripé não será capaz de libertar a economia brasileira de outra "possível trindade": altas taxas de juros reais, a apreciação da taxa de câmbio real e crescimento econômico muito baixo. Depois de analisar brevemente a base teórica sob o tripé macroeconômico, o artigo mostra por que este regime de política macroeconômica, se avaliado numa perspectiva de longo ou médio prazo, não tem sido capaz de garantir a estabilidade dos preços nem o crescimento econômico. Além da sugestão de romper com a estratégia brasileira de crescer com poupança externa, o documento também sugere três principais formas de mudar o modus operandi do tripé brasileiro: i aumentar o horizonte de tempo para atingir a meta de inflação, como tem sido o experiência da maioria dos países que adotam esse regime de política monetária; ii restaurar o papel anticíclico da política fiscal brasileira; e iii adotar uma combinação de mecanismos que visem prevenir que a moeda brasileira entre em uma nova tendência cíclica da apreciação em termos reais.

  2. Desarrollo vocacional y política pública

    OpenAIRE

    Watts, Anthony G.

    2000-01-01

    El trabajo analiza la base para el interés político en los servicios de desarrollo vocacional, y la manera en la cual esta base está siendo fortalecida por las actuales transformaciones en el trabajo y en el desarrollo vocacional. Se exploran los papeles potenciales de la política pública en los servicios de desarrollo vocacional así como las formas en las que dichos servicios pueden influenciar el proceso de la creación de políticas. Se identifican una gama de temas de política relacionados ...

  3. Política, violencia y literatura

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    Kohut, Karl

    2002-06-01

    Full Text Available Tomando como punto de partida la opinión general de que América Latina es el continente violento por excelencia y la hipótesis defendida por Ariel Dorfman (1972 se analiza aquí la cuestión de la violencia política, ciñéndose a la literatura de la segunda mitad del siglo XX. Tras discutir las definiciones (divergentes de la violencia propuestas por la filosofía política, se pasa a una fenomenología de la violencia en la literatura, examinando hasta qué punto y en qué sentido ésta sería el carácter distintivo de la literatura latinoamericana. Finalmente, se analiza la metarreflexión sobre la violencia política en la literatura ficcional, centrándose en tres puntos: (1 el (presunto ser violento del mundo latinoamericano, (2 la violencia estatal, y (3 la violencia de la resistencia. Se constata, entre otras cosas, que ha habido una tendencia a atacar la violencia estatal y defender la de la resistencia; tendencia comprensible en tiempos de dictadura, pero que se vuelve problemática bajo una democracia. Dos preguntas cierran este trabajo: hasta qué punto la violencia es un fenómeno de la cultura, que crece en su mismo seno, y no un fenómeno extracultural dirigido contra ella, y hasta dónde la libertad individual y la seguridad cívica pueden ser conciliadas, punto que ha ocupado un lugar central en la discusión internacional después de los acontecimientos del 11 de septiembre.

  4. La polémica del nacionalismo

    Directory of Open Access Journals (Sweden)

    Germán Posada

    1961-10-01

    Full Text Available La polémica empezó con el artículo de Hernando Téllez, hace veinte días. Con el pretexto de defender a los escritores de su propia generación, a quienes, según él, se viene acusando sistemáticamente de producir una literatura desarraigada y ausente de la vida nacional, Téllez se lanzó a combatir las teorías de " nacionalismo literario'' que actualmente se agitan en Colombia.

  5. Dos Partidos Políticos

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    Marquessuel Dantas de Souza

    2017-09-01

    Full Text Available A presente resenha se refere ao interessante texto de Simone Weil (Note sur la suppression générale des partis politiques - de 1940, escrito em Londres, traduzido e publicado pela iniciativa audaciosa da Editora Ayiné. Originalmente publicado na coletânea intitulada ÉCRITS DE LONDRES et dernières lettres - Éditions Gallimard (1957, este texto apresenta de forma singular o que significa um partido político; algo de muita importância para e na vida social do homem moderno.

  6. Amor y política.

    Directory of Open Access Journals (Sweden)

    Hernando Bernal.

    1998-09-01

    Full Text Available Este trabajo pretende hacer una serie de consideraciones que apuntan sobre todo a extraer una definición de lo que es «la política» para el psicoanálisis a partir de una observación que hace Jacques Alain Miller en su texto Lógicas de la vida amorosa sobre cómo Freud introduce una «teoría política» a partir de su texto Psicología de las masas y análisis del yo.En dicho texto Freud hace ver el poder ordenador y apaciguador del amor significante amo en la medida en que una masa no es más que el amor uniendo a muchas personas y reiterado en cada una de ellas. Las fuerzas armadas y la Iglesia son un buen ejemplo de ello.Pero a pesar de la cohesión amorosa de la humanidad por el poder unificante del amor, resta siempre un malestar. El malestar que persiste en la cultura testimonia del fracaso del amor para resolver el empuje del hombre a satisfacerse con el mal. El significante amo no parece entonces solucionar la paradoja del goce. De aquí que preguntarse sobre adónde vaya el goce en el orden social sea también, para el psicoanálisis, una cuestión política. Jacques- Alain Miller observa, en su texto Lógicas de la vida amorosa, cómo, a partir de la Psicología de las masas y análisis del yo, se puede extraer de Freud una «teoría política», que se vincula muy estrechamente con el amor. Esto porque La Psicología de las masas... enseña sobre el poder ordenador y apaciguador del amor, o mejor, del significante «amor» como significante Amo, lo que se puede escribir de la siguiente manera con ayuda de la «teoría de los discursos» o «teoría del vínculo social» de Lacan.

  7. Políticas de reconhecimento uma novidade das políticas sociais do PT?

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    Krischke, Paulo Jose Duval da Silva

    2004-01-01

    Full Text Available O presente trabalho levanta a hipótese de que as ações de governo do PT estão inovando significativamente na necessária convergência (cf. Fraser, 1999; Honnetch, 2003 entre as tradicionais políticas de redistribuição sócio-econômica, e uma nova diretriz de reconhecimento das diferenças sócio-políticas e culturais. O argumento retoma a trajetória sindical do PT e os efeitos das políticas participativas, nas mudanças da cultura política em várias partes do país. Entre esses efeitos, o crescente apoio à democracia parece relacionar-se ao reconhecimento do direito à diferença e ao pluralismo - principalmente entre a juventude e naqueles locais onde o PT tem governado na última década

  8. Dinâmica Institucional, Políticas Públicas e o Desempenho Político Ambiental Brasileiro

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    Diego de Freitas Rodrigues

    2011-12-01

    Full Text Available Este estudo buscou analisar os efeitos da interdependência entre instituições políticas e modelos de desenvolvimento no desempenho de políticas ambientais no Brasil. A hipótese central do trabalho é que, dada a interdependência entre os organismos responsáveis pelas políticas ambientais e padrões de desenvolvimento pouco responsivos à complexidade ambiental, ocorre uma dispersão de poder decisório incentivando o modelo de desenvolvimento de alto carbono. Além de revisão da literatura em Políticas Públicas e Economia Ecológica, foram operacionalizadas (1 uma análise do desenho político-institucional brasileiro e (2 o quadro de gastos públicos do governo federal brasileiro com meio ambiente. Dois resultados foram observados: 1o o desenho e as competências das instituições políticas responsáveis pela formulação e implementação de políticas públicas interferem na maior eficiência de políticas ambientais; 2o quanto maior a abertura institucional, maior a tendência de paralisia decisória na formulação e implementação de políticas públicas ambientais.

  9. Croce, Gramsci e a "autonomia da política"

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    Álvaro Bianchi

    2007-11-01

    Full Text Available Na reflexão que Gramsci desenvolveu nos Quaderni del carcere, o tema da autonomia da política ocupa uma importante posição. Foi com base nessa reflexão que Gramsci desenvolveu sua pesquisa a respeito de política e da possibilidade de uma ciência política. Segundo Benedetto Croce, cabia a Nicolau Maquiavel o mérito de ter afirmado pela primeira vez a autonomia da política. Para Croce, essa autonomia permitia estabelecer uma distinção radical entre ética e política e entre "filosofia da política" e "ciência empírica da política". Gramsci tomou criticamente a reflexão croceana como ponto de partida de sua leitura de Maquiavel. O reconhecimento da autonomia da política implicava que esta não poderia ser reduzida à religião ou à ética. Como campo do conhecimento e como atividade, a ciência política e a política tinham regras próprias, que as distinguiam de outras formas do conhecimento e da atividade humana. Mas tal "autonomia" não implicava, para o marxista sardo, uma separação radical entre política e moral. Por essa razão, Gramsci encontrava em Maquiavel um precursor da filosofia da práxis em sentido pleno, ou seja, o criador de uma "ciência-ação revolucionária".

  10. Cultura Política na Antiguidade

    Directory of Open Access Journals (Sweden)

    José Petrúcio Farias Júnior

    2009-06-01

    Full Text Available

    Pretendemos discutir nesse artigo algumas implicações do termo cultura política na Antiguidade, especificamente, na sociedade romana imperial. Dessa forma, acreditamos que as considerações que apresentaremos a seguir contribuam para reflexão da utilização desse termo, produto das investigações contemporâneas, sobre os estudos das sociedades antigas. Para a historiadora Profa. Dra. Margarida Maria de Carvalho, tais reflexões representam ainda uma lacuna na historiografia concernente a essa temática. Isso posto, discorreremos, inicialmente, sobre o conceito de cultura e seus desdobramentos para, em seguida, refletirmos sobre o viés interpretativo que fundamenta estudos históricos que se desenvolvem sob o beneplácito da linha de pesquisa intitulada História e Cultura Política.

  11. Adeus à política

    Directory of Open Access Journals (Sweden)

    Manoel Mendonça Filho

    2012-01-01

    Full Text Available O artigo trata as questões do "Estado" e da "Política" partindo do exemplo concreto do processo de institucionalização, no Brasil, de práticas profissionais em psicologia reconhecidas como Análise Institucional (vertente grupalista francesa consideravelmente difundida nos últimos 20 anos. Trabalho para expor uma face menos visível da judicialização que funciona ao nível das concepções, crenças e valores entre os operadores dos equipamentos onde se materializam as políticas públicas engendradas pelo excesso legalista. Dentre as modalidades desse apego à lei, toma-se em análise aquela mais próxima de nossas práticas de funcionalismo público marcando o índice de grau máximo de sua institucionalização: a aceitação consensual da competência como critério de legitimidade na operação dos "instrumentos da violência institucional".

  12. Política de los intelectuales

    Directory of Open Access Journals (Sweden)

    Todorov Tzevetan

    1998-06-01

    Full Text Available ¿Qué es un intelectual? Por lo que a mi respecta, limito el uso de este término de la siguiente manera: es un estudioso o un artista (categoria que incluye a los escritores que no se contenta con realizar trabajos cientificos o con la creación de una obra de arte, que no se contenta, por lo tanto, con una méra búsqueda de lo verdadero y con un méro desarrollo de lo bello, sino que siente asimismo comprometido con la noción de bienestar público, con los valores de la sociedad en la que vive, es alguien que participa en el debate sobre esos mismos valores. El intelectual así entendido se halla muy lejos del artista o del estudioso a quienes no preocupan en absoluto las dimensiones políticas o éticas de sus obras; muy lejos también, del predicador o del político profesional, que no crean obra alguna.

  13. Metallothionein (MT)-III

    DEFF Research Database (Denmark)

    Carrasco, J; Giralt, M; Molinero, A

    1999-01-01

    Metallothionein-III is a low molecular weight, heavy-metal binding protein expressed mainly in the central nervous system. First identified as a growth inhibitory factor (GIF) of rat cortical neurons in vitro, it has subsequently been shown to be a member of the metallothionein (MT) gene family...... injected rats. The specificity of the antibody was also demonstrated in immunocytochemical studies by the elimination of the immunostaining by preincubation of the antibody with brain (but not liver) extracts, and by the results obtained in MT-III null mice. The antibody was used to characterize...... the putative differences between the rat brain MT isoforms, namely MT-I+II and MT-III, in the freeze lesion model of brain damage, and for developing an ELISA for MT-III suitable for brain samples. In the normal rat brain, MT-III was mostly present primarily in astrocytes. However, lectin staining indicated...

  14. Human PrimPol activity is enhanced by RPA.

    Science.gov (United States)

    Martínez-Jiménez, María I; Lahera, Antonio; Blanco, Luis

    2017-04-10

    Human PrimPol is a primase belonging to the AEP superfamily with the unique ability to synthesize DNA primers de novo, and a non-processive DNA polymerase able to bypass certain DNA lesions. PrimPol facilitates both mitochondrial and nuclear replication fork progression either acting as a conventional TLS polymerase, or repriming downstream of blocking lesions. In vivo assays have shown that PrimPol is rapidly recruited to sites of DNA damage by interaction with the human replication protein A (RPA). In agreement with previous findings, we show here that the higher affinity of RPA for ssDNA inhibits PrimPol activities in short ssDNA templates. In contrast, once the amount of ssDNA increases up to a length in which both proteins can simultaneously bind ssDNA, as expected during replicative stress conditions, PrimPol and RPA functionally interact, and their binding capacities are mutually enhanced. When using M13 ssDNA as template, RPA stimulated both the primase and polymerase activities of PrimPol, either alone or in synergy with Polε. These new findings supports the existence of a functional PrimPol/RPA association that allows repriming at the exposed ssDNA regions formed in the leading strand upon replicase stalling.

  15. O conceito de liberdade política em Montesquieu

    Directory of Open Access Journals (Sweden)

    Maria Beatriz Nizza da Silva

    1969-06-01

    Full Text Available O primeiro passo na análise do conceito de liberdade política, tal como êle se nos apresenta em De l'esprit des lois, deverá assina-lar uma diferença, fundamental para Montesquieu, entre "liberdade filosófica" e "liberdade política".

  16. Los spots factor, esencial del marketing político

    Directory of Open Access Journals (Sweden)

    María de Jesús Origel Gutiérrez

    2000-01-01

    Full Text Available EI objetivo del presente trabajo es demostrar que en las elecciones presidenciales de julio de 2000, la forma de hacer política en México se modificó al introducir en las campanas electorales el marketing político y nuevas estrategias de comunicación, en especial los spots televisivos

  17. Survey of Current US Army POL Doctrine, Procedures, Personnel, and Equipment for the Supply and Inland Distribution of Bulk POL

    Science.gov (United States)

    1985-10-01

    Refueling Under Armor ......................... 34 Page i ,t .J APPENDICES A. POL Unit Missions and Capabilities ....................... A-I B. Roster...refueling Open versus closed port refueling Requirement for Refueling Under Armor Robotic refueler V % Page 34 S.< ’p. ". APPgeDI A-"" • " ’ ’ POL " it

  18. A política da Igreja Universal e seus reflexos nos campos religioso e político brasileiros

    Directory of Open Access Journals (Sweden)

    Oro Ari Pedro

    2003-01-01

    Full Text Available Este texto versa sobre a inserção da Igreja Universal do Reino de Deus (IURD na política nacional e seus efeitos nos campos religioso e político. Em razão da eficácia de seu carisma institucional, a Universal procedeu, dentro do próprio grupo religioso, a uma ressemantização do ato de votar em particular e da percepção da política em geral, inscrevendo-os em sua lógica religiosa. Isso é uma importante chave explicativa para o elevado grau de fidelidade de votos e o êxito político cada vez maior constatado nas últimas eleições. Ademais, a prática política da Universal está produzindo um efeito mimético em outra igrejas evangélicas, que tendem a imitar seu modelo de fazer política. Sua inserção política, sobretudo por intermédio do Partido Liberal, não passa despercebida pelos partidos, constituindo-se, assim, em um ator relevante na atual conjuntura política brasileira.

  19. Estudo Pré-Clínico de Stent com Polímero Biodegradável e Liberação Abluminal de Sirolimus

    Directory of Open Access Journals (Sweden)

    Celso Kiyochi Takimura

    2014-06-01

    Full Text Available Fundamento: Stents recobertos com polímeros bioabsorvíveis e fármacos apenas na face abluminal podem ser mais seguros que stents farmacológicos com polímeros permanentes. Objetivo: Relatar os resultados experimentais com o stent Inspiron(r, um stent recoberto com polímero bioabsorvível e com liberação de sirolimus apenas da face abluminal, recentemente aprovado para uso clínico. Métodos: Foram implantados 45 stents nas artérias coronárias de 15 porcos e, no 28° dia pós-implante, foram obtidos os resultados angiográficos, de ultrassonografia intracoronária e de histomorfologia. Cinco grupos foram avaliados: Grupo I (nove stents sem recobrimento; Grupo II (nove stents com polímero bioabsorvível nas faces luminal e abluminal; Grupo III (oito stents com polímero bioabsorvível na face abluminal; Grupo IV (nove stents com polímero bioabsorvível e sirolimus nas faces luminal e abluminal; e Grupo V (dez stents com polímero bioabsorvível e sirolimus na face abluminal exclusivamente. Resultados: Observamos, para os Grupos I, II, III, IV e V respectivamente: porcentual de estenose de 29 ± 20; 36 ± 14; 33 ± 19; 22 ± 13 e 26 ± 15 (p = 0,443; perda luminal tardia (em mm de 1,02 ± 0,60; 1,24 ± 0,48; 1,11 ± 0,54; 0,72 ± 0,44 e 0,78 ± 0,39 (p = 0,253; área neointimal (em mm2 de 2,60 ± 1,99; 2,74 ± 1,51; 2,74 ± 1,30; 1,30 ± 1,14 e 0,97 ± 0,84 (p = 0,001; Grupos IV e V versus Grupos I, II e III e porcentual de área neointimal de 35 ± 25; 38 ± 18; 39 ± 19; 19 ± 18 e 15 ± 12 (p = 0,001; Grupo IV e V versus Grupo I, II e III. Os escores de injúria e inflamação foram baixos e sem diferenças entre os grupos. Conclusão: O stent Inspiron(r foi seguro e inibiu significativamente a hiperplasia neointimal observada no 28° dia pós-implante em artérias coronárias porcinas.

  20. SOUTH POL: Revealing the Polarized Southern Sky

    Science.gov (United States)

    Magalhaes, Antonio Mario Mario; Ramírez, Edgar; Ribeiro, Nadili; Seriacopi, Daiane; Rubinho, Marcelo; Ferrari, Tiberio; Rodrigues, Claudia; Schoenell, William; Herpich, Fabio; Pereyra, Antonio

    2018-01-01

    SOUTH POL will be a survey of the Southern sky in optical polarized light. It will use a newly built polarimeter for T80-S, an 84 cm robotic telescope installed at Cerro Tololo (CTIO), Chile. It will initially cover the sky South of declination -15 deg with a polarimetric accuracy Solar System.The polarimeter has just been commissioned in mid-November, 2017. The data reduction pipeline has already been built. We will describe the instrument and the data reduction, as well as a few of the science cases. The survey is expected to begin midway through the 1st semester of 2018. Both catalog data and raw images will be made available.

  1. Cultura e políticas sociais : uma visão a partir da cultura política

    OpenAIRE

    Corrêa, Helena Ariane Borges

    2008-01-01

    A dissertação parte do questionamento de por que fatores culturais não são considerados na formulação de políticas públicas. Para tanto são analisadas e discutidas duas vertentes tradicionalmente separadas na ciência política: culturalismo e institucionalismo, que aqui serão trabalhadas em um nível de análise menos abstrato, relacionando elementos de cultura política com políticas públicas. Com isto o trabalho procura mostrar a relevância do estudo de fatores culturais em políticas sociais e ...

  2. NNDSS - Table III. Tuberculosis

    Data.gov (United States)

    U.S. Department of Health & Human Services — NNDSS - Table III. Tuberculosis - 2018.This Table includes total number of cases reported in the United States, by region and by states, in accordance with the...

  3. NNDSS - Table III. Tuberculosis

    Data.gov (United States)

    U.S. Department of Health & Human Services — NNDSS - Table III. Tuberculosis - 2017.This Table includes total number of cases reported in the United States, by region and by states, in accordance with the...

  4. Workshop 96. Part III

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-31

    Part III of the proceedings contain 155 contributions in various fields of science and technology including nuclear engineering, environmental science, and biomedical engineering. Out of these, 10 were selected to be inputted in INIS. (P.A.).

  5. Workshop 96. Part III

    International Nuclear Information System (INIS)

    1995-12-01

    Part III of the proceedings contain 155 contributions in various fields of science and technology including nuclear engineering, environmental science, and biomedical engineering. Out of these, 10 were selected to be inputted in INIS. (P.A.)

  6. Delineation of the Exact Transcription Termination Signal for Type 3 Polymerase III

    Directory of Open Access Journals (Sweden)

    Zongliang Gao

    2018-03-01

    Full Text Available Type 3 Pol III promoters such as U6 are widely used for expression of small RNAs, including short hairpin RNA for RNAi applications and guide RNA in CRISPR genome-editing platforms. RNA polymerase III uses a T-stretch as termination signal, but the exact properties have not been thoroughly investigated. Here, we systematically measured the in vivo termination efficiency and the actual site of termination for different T-stretch signals in three commonly used human Pol III promoters (U6, 7SK, and H1. Both the termination efficiency and the actual termination site depend on the T-stretch signal. The T4 signal acts as minimal terminator, but full termination efficiency is reached only with a T-stretch of ≥6. The termination site within the T-stretch is quite heterogeneous, and consequently small RNAs have a variable U-tail of 1–6 nucleotides. We further report that such variable U-tails can have a significant negative effect on the functionality of the crRNA effector of the CRISPR-AsCpf1 system. We next improved these crRNAs by insertion of the HDV ribozyme to avoid U-tails. This study provides detailed design guidelines for small RNA expression cassettes based on Pol III.

  7. A Polícia e suas Polícias: Clientela, Hierarquia, Soldado e Bandido

    Directory of Open Access Journals (Sweden)

    Erika Ferreira de Azevedo

    Full Text Available Resumo A polícia militar representa um braço do Estado e apresenta-se para a população de forma contundente, ostensiva e, por vezes, brutal, não só para a sociedade como para ela mesma. Através da Análise Institucional do Discurso, a pesquisa aqui relatada teve como objetivo estudar os efeitos de reconhecimento e desconhecimento das relações no trabalho que permeiam o discurso de soldados da polícia militar: como estes falam de seu trabalho e, através desta fala, posicionam-se e posicionam sua clientela, sua hierarquia, seu objeto de trabalho (os “bandidos” e eles próprios. Buscou-se também analisar que lugar a violência ocupou neste discurso. Dez soldados da polícia militar do Estado de São Paulo foram entrevistados e a transcrição destas estrevistas foi analisada. A partir das análises, é possível refletir sobre a dubiedade do verdadeiro objetivo do trabalho policial, deslizando facilmente do cuidado da população desamparada ao cuidado de si, desamparado sob a pressão da farda.

  8. A structural role for the PHP domain in E. coli DNA polymerase III.

    Science.gov (United States)

    Barros, Tiago; Guenther, Joel; Kelch, Brian; Anaya, Jordan; Prabhakar, Arjun; O'Donnell, Mike; Kuriyan, John; Lamers, Meindert H

    2013-05-14

    In addition to the core catalytic machinery, bacterial replicative DNA polymerases contain a Polymerase and Histidinol Phosphatase (PHP) domain whose function is not entirely understood. The PHP domains of some bacterial replicases are active metal-dependent nucleases that may play a role in proofreading. In E. coli DNA polymerase III, however, the PHP domain has lost several metal-coordinating residues and is likely to be catalytically inactive. Genomic searches show that the loss of metal-coordinating residues in polymerase PHP domains is likely to have coevolved with the presence of a separate proofreading exonuclease that works with the polymerase. Although the E. coli Pol III PHP domain has lost metal-coordinating residues, the structure of the domain has been conserved to a remarkable degree when compared to that of metal-binding PHP domains. This is demonstrated by our ability to restore metal binding with only three point mutations, as confirmed by the metal-bound crystal structure of this mutant determined at 2.9 Å resolution. We also show that Pol III, a large multi-domain protein, unfolds cooperatively and that mutations in the degenerate metal-binding site of the PHP domain decrease the overall stability of Pol III and reduce its activity. While the presence of a PHP domain in replicative bacterial polymerases is strictly conserved, its ability to coordinate metals and to perform proofreading exonuclease activity is not, suggesting additional non-enzymatic roles for the domain. Our results show that the PHP domain is a major structural element in Pol III and its integrity modulates both the stability and activity of the polymerase.

  9. A questão da continuidade da política macroeconômica entre o governo Cardoso e Lula (1995-2006

    Directory of Open Access Journals (Sweden)

    José Marcos Nayme Novelli

    2010-06-01

    Full Text Available O objetivo do artigo é compreender a continuidade da política macroeconômica entre o governo Cardoso e o governo Lula. O artigo organiza-se em três seções. Na primeira, descreve-se sucintamente a política macroeconômica dos governos Cardoso e Lula. Na segunda, apresenta-se e discute-se o debate da literatura especializada a respeito da hipótese da continuidade da política macroeconômica entre os dois governos. Na terceira seção, é feito um mapeamento dos dirigentes estatais, em que ressaltamos certos aspectos que contribuíram para tal continuidade entre os dois governos. Sem desconsiderar a ação dos beneficiários últimos dos resultados de tal política, identificamos três fatores para explicar a manutenção da política macroeconômica: i a própria estrutura do capitalismo brasileiro e sua inserção na economia mundial; ii a força das idéias ortodoxas difundidas pela mídia e dominantes na equipe econômica (Ministério da Fazenda e Banco Central do Brasil, que foi recrutada no mesmo "campo"; iii a aceitação das "regras do jogo" da democracia pelo Partido dos Trabalhadores (PT.

  10. La memoria social y la memoria política

    OpenAIRE

    Lifschitz, Javier Alejandro

    2012-01-01

    El artículo trata sobre la constitución del campo de la memoria política. Discute inicialmente la perspectiva de la memoria social y aborda, a partir de autores como Bourdieu y Habermas, la idea de un campo de la memoria política. Identifica dos momentos de inflexión en la constitución de ese campo. Un primer momento, en que la memoria política se inscribe en los procesos estatales de construcción de una memoria nacional y un segundo momento, que tiene como referencia principalmente el contex...

  11. Arma del contrapoder: Humor político y medios

    OpenAIRE

    César Ulloa Tapia

    2008-01-01

    El humor político en los medios de comunicación tiene enorme acogida en las audiencias ya que logra lo que pueden editoriales, análisis académicos y demás espacios de opinión. Es la mejor manera de denunciar, advertir, sancionar e incluso develar lo que hace o deja de hacer el poder político. El humor político está considerado como contrapoder. El periodismo que usa el humor es eficaz, tanto en el mensaje como en su efecto, pues describe las situaciones y recurre a comparaciones y símiles....

  12. Fusion Power Demonstration III

    International Nuclear Information System (INIS)

    Lee, J.D.

    1985-07-01

    This is the third in the series of reports covering the Fusion Power Demonstration (FPD) design study. This volume considers the FPD-III configuration that incorporates an octopole end plug. As compared with the quadrupole end-plugged designs of FPD-I and FPD-II, this octopole configuration reduces the number of end cell magnets and shortens the minimum ignition length of the central cell. The end-cell plasma length is also reduced, which in turn reduces the size and cost of the end cell magnets and shielding. As a contiuation in the series of documents covering the FPD, this report does not stand alone as a design description of FPD-III. Design details of FPD-III subsystems that do not differ significantly from those of the FPD-II configuration are not duplicated in this report

  13. La ciencia política, ciencia noética del orden.

    Directory of Open Access Journals (Sweden)

    Felipe Cárdenas Támara.

    2010-07-01

    Full Text Available This work has the following fundamental purposes: i to respond by way of a critical review to the statements on political science's object of study made by Rodrigo Losada and Andrés Casas in their recently published book Enfoques para el análisis político. Historia, epistemología y perspectivas de la ciencia política (Approaches for Political Analysis. History, Epistemology and Perspectives of Political Science, 2008; ii to reflect on the theoretical reductionism in the conceptualization of the object of study of political science, present in some contemporary schools of thought, and, iii to suggest the need to expand our understanding of our political reality from the intellectual perspective proposed by Eric Voegelin, who argued that political science is a noetic scientific discipline centered on the study of order and the experience of order in societies and human cultures. The contribu-tion of the article refers to the reconstitution and resignification of political science as a scientific noetic discipline and to the multiple transdisciplinary fields that such a condition makes possible.

  14. Lo social desde la política

    Directory of Open Access Journals (Sweden)

    Darío Salinas Figueredo

    2000-01-01

    Full Text Available Sin desconocer las aproximaciones existentes sobre aspectos fundamentales de la problemática social en América Latina, por ejemplo la pobreza y la índole de política que de manera predominante se formula para encararla, la trayectoria argumental de este trabajo se encamina a mostrar la importancia de trabajar hacia el desarrollo de una perspectiva más amplia. Las referencias empíricas cotejadas no se refieren a hechos puntuales, sino a indicadores que pautan tendencias. Siendo los propósitos aquí reunidos de naturaleza comprensiva, se parte de la premisa según la cual escasamente se problematiza lo social y la propia política social, sus vínculos con la política general, el modelo de desarrollo y el tipo de democracia que la política predominante busca proyectar.

  15. III-V microelectronics

    CERN Document Server

    Nougier, JP

    1991-01-01

    As is well known, Silicon widely dominates the market of semiconductor devices and circuits, and in particular is well suited for Ultra Large Scale Integration processes. However, a number of III-V compound semiconductor devices and circuits have recently been built, and the contributions in this volume are devoted to those types of materials, which offer a number of interesting properties. Taking into account the great variety of problems encountered and of their mutual correlations when fabricating a circuit or even a device, most of the aspects of III-V microelectronics, from fundamental p

  16. Murine mammary tumor virus pol-related sequences in human DNA: characterization and sequence comparison with the complete murine mammary tumor virus pol gene

    International Nuclear Information System (INIS)

    Deen, K.C.; Sweet, R.W.

    1986-01-01

    Sequences in the human genome with homology to the murine mammary tumor virus (MMTV) pol gene were isolated from a human phage library. Ten clones with extensive pol homology were shown to define five separate loci. These loci share common sequences immediately adjacent to the pol-like segments and, in addition, contain a related repeat element which bounds this region. This organization is suggestive of a proviral structure. The authors estimate that the human genome contains 30 to 40 copies of these pol-related sequences. The pol region of one of the cloned segments (HM16) and the complete MMTV pol gene were sequenced and compared. The nucleotide homology between these pol sequences is 52% and is concentrated in the terminal regions. The MMTV pol gene contains a single long open reading frame encoding 899 amino acids and is demarcated from the partially overlapping putative gag gene by termination codons and a shift in translational reading frame. The pol sequence of HM16 is multiply terminated but does contain open reading frames which encode 370, 105, and 112 amino acids residues in separate reading frames. The authors deduced a composite pol protein sequence for HM16 by aligning it to the MMTV pol gene and then compared these sequences with other retroviral pol protein sequences. Conserved sequences occur in both the amino and carboxyl regions which lie within the polymerase and endonuclease domains of pol, respectively

  17. Politeia: la política

    Directory of Open Access Journals (Sweden)

    Manuel Briceño Jáuregui

    1982-07-01

    Full Text Available Se trata solamente de La Política, una versión reciente que está en proceso de publicación. Aristóteles sostiene que la felicidad completa del individuo no es posible sino en la sociedad, especialmente en la polis, que es la sociedad por excelencia. El individuo no puede por sí mismo satisfacer sus necesidad es materiales ni alcanzar la virtud. Para mostrarlo examina primero los diferentes grupos humanos, a partir de la familia; analiza luego las diversas politeias (constituciones de entonces, algunas de las cuales condena. Distingue siguiendo a su maestro Platón tres politeias (formas de gobierno legítimas monarquía, aristocracia, democracia, que dan lugar a su vez a tres desviaciones de las mismas tiranía , oligarquía y demagogia respectivamente. Termina señalando la importancia humana y cívica de la educación.

  18. Política y Geometría

    Directory of Open Access Journals (Sweden)

    Isabel Lillo Villalobos

    2011-04-01

    Full Text Available En este trabajo se aplican conceptos matemáticos al planteamiento deun problema de competición política. Se desarrolla y se implementaademás un método que usa herramientas relacionadas con la geometríacomputacional para la resolución de dicho problema, probándose dicho método en un ejemplo basado en la política española.

  19. O neopragmatismo de Richard Rorty e a reflexão política contemporânea

    Directory of Open Access Journals (Sweden)

    Sérgio Eduardo Ferraz

    2014-03-01

    Full Text Available Este artigo examina o pensamento filosófico de Richard Rorty e, em particular, sua dimensão política, delineando seu tratamento original e crítico de temas centrais à teoria política contemporânea. O objetivo do texto é triplo: chamar a atenção para a fertilidade das ideias de um autor ainda pouco lido no Brasil (i, assinalar as notáveis consequências corrosivas da abordagem rortyana para pressupostos e pretensões fundantes de importantes vertentes da teoria política (ii, e, numa ótica mais positiva, apontar o potencial inspirador dos escritos desse autor para a reflexão sobre novos modos de justificação da democracia (iii. Salientam-se também, nesse último caso, os limites impostos a esse tipo de empreitada político-intelectual. A abordagem empregada consiste em demarcar os contornos gerais do projeto filosófico do autor, seguindo-se uma apresentação dos tópicos mais propriamente políticos da obra de Rorty. Mais especificamente, o artigo explora a proposta rortyana de substituição da noção de verdade pela de justificação, assinalando-se suas consequências para pretensões de verdade, objetividade e universalismo, presentes em diversas linhas da teoria política. Nesse veio, o artigo discute as eventuais implicações relativistas das teses rortyanas, chegando-se, por essa rota, às ideias de contingência, imaginação e persuasão, núcleos conceituais vitais à reflexão em tela. Um breve contraste com posições de autores como Habermas e Rawls, caros ao próprio Rorty, é esboçado no propósito de tornar mais clara a especificidade da abordagem rortyana da política. Transitando, do modo assinalado, por esses tópicos, o texto resulta em um panorama sintético da perspectiva rortyana acerca do que vem a ser, contemporaneamente, uma "política democrática", liberta de "universalismos" e "objetividades", na qual ganham estatuto decisivo questões de justificação, tolerância e coexist

  20. Cultura política y partidos en Jalisco

    Directory of Open Access Journals (Sweden)

    Carlos Silva Moreno

    2000-01-01

    Full Text Available El presente trabajo es un intento de análisis y reflexión sobre la cultura política de uno de los componentes de todo sistema político que se precie de ser democrático (al menos formalmente: los partidos políticos. Se estudia la cultura política de los partidos políticos en Jalisco a partir del análisis de las representaciones y valores que los partidos políticos tienen sobre la democracia interna, para después contrastarlas con algunas prácticas referentes al liderazgo, dirección y formas de competencia. Debido a que las dimensiones de la democracia son muy amplias y variadas, nos centramos en dos indicadores: la forma en que se da la competencia por los cargos de dirección interna y el carácter de la dirección y el liderazgo.

  1. Fluctuations of pol I and fibrillarin contents of the nucleoli.

    Science.gov (United States)

    Hornáček, M; Kováčik, L; Mazel, T; Cmarko, D; Bártová, E; Raška, I; Smirnov, E

    2017-07-04

    Nucleoli are formed on the basis of ribosomal DNA (rDNA) clusters called Nucleolus Organizer Regions (NORs). Each NOR contains multiple genes coding for RNAs of the ribosomal particles. The prominent components of the nucleolar ultrastructure, fibrillar centers (FC) and dense fibrillar components (DFC), together compose FC/DFC units. These units are centers of rDNA transcription by RNA polymerase I (pol I), as well as the early processing events, in which an essential role belongs to fibrillarin. Each FC/DFC unit probably corresponds to a single transcriptionally active gene. In this work, we transfected human-derived cells with GFP-RPA43 (subunit of pol I) and RFP-fibrillarin. Following changes of the fluorescent signals in individual FC/DFC units, we found two kinds of kinetics: 1) the rapid fluctuations with periods of 2-3 min, when the pol I and fibrillarin signals oscillated in anti-phase manner, and the intensities of pol I in the neighboring FC/DFC units did not correlate. 2) fluctuations with periods of 10 to 60 min, in which pol I and fibrillarin signals measured in the same unit did not correlate, but pol I signals in the units belonging to different nucleoli were synchronized. Our data indicate that a complex pulsing activity of transcription as well as early processing is common for ribosomal genes.

  2. Herencia política y económica

    Directory of Open Access Journals (Sweden)

    Wesley C. Marshall

    2011-01-01

    Full Text Available El artículo busca resaltar la relevancia de la vida política de Hilferding en el actual contexto de la crisis financiera global y la probablemente alta radicalización de las posiciones políticas de los países dominantes como resultado de aquella. Como se argumenta, había un alto grado de coherencia entre el pensamiento económico de Hilferding y su actuación política. Justamente por el hecho de que el proyecto socialista alemán durante la Republica Weimar no logró sus objetivos, el pensamiento político y económico de su líder intelectual es altamente relevante en el escenario global actual, donde la crisis económica y descomposición social y política que la acompañen reclaman a los principales políticos del mundo un entendimiento económico más amplio y profundo para que las tragedias de Europa central de los años treinta y cuarenta del siglo XX no se reproduzcan.

  3. Mentir en la vida política

    Directory of Open Access Journals (Sweden)

    Martínez de la Escalera, Ana María

    2005-06-01

    Full Text Available In this paper the act of lying is seen as a necessary component of the political discourse of the polis. Plato and Arendt are examined as the representatives of a line of thought in which the political realm needs the act of lying. The actual problematic of lying in politics is debated.

    El texto «Mentir en política» aborda el papel que la mentira ha jugado en el lenguaje de la política y el lugar problemático que la Filosofía política le ha otorgado. Se escogen dos autores, Platón y Arendt, para mostrar la necesidad de incluir el discurso mentiroso como un componente esencial de la vida política de la ciudad. En breve, se trata de analizar los límites que la ciudad impone al discurso de lo político y de la política.

  4. Mitochondrial DNA replication: a PrimPol perspective

    Science.gov (United States)

    Bailey, Laura J.

    2017-01-01

    PrimPol, (primase–polymerase), the most recently identified eukaryotic polymerase, has roles in both nuclear and mitochondrial DNA maintenance. PrimPol is capable of acting as a DNA polymerase, with the ability to extend primers and also bypass a variety of oxidative and photolesions. In addition, PrimPol also functions as a primase, catalysing the preferential formation of DNA primers in a zinc finger-dependent manner. Although PrimPol's catalytic activities have been uncovered in vitro, we still know little about how and why it is targeted to the mitochondrion and what its key roles are in the maintenance of this multicopy DNA molecule. Unlike nuclear DNA, the mammalian mitochondrial genome is circular and the organelle has many unique proteins essential for its maintenance, presenting a differing environment within which PrimPol must function. Here, we discuss what is currently known about the mechanisms of DNA replication in the mitochondrion, the proteins that carry out these processes and how PrimPol is likely to be involved in assisting this vital cellular process. PMID:28408491

  5. Summary of Session III

    International Nuclear Information System (INIS)

    Furman, M.A.

    2002-01-01

    This is a summary of the talks presented in Session III ''Simulations of Electron-Cloud Build Up'' of the Mini-Workshop on Electron-Cloud Simulations for Proton and Positron Beams ECLOUD-02, held at CERN, 15-18 April 2002

  6. Prediction of Active Site and Distal Residues in E. coli DNA Polymerase III alpha Polymerase Activity.

    Science.gov (United States)

    Parasuram, Ramya; Coulther, Timothy A; Hollander, Judith M; Keston-Smith, Elise; Ondrechen, Mary Jo; Beuning, Penny J

    2018-02-20

    The process of DNA replication is carried out with high efficiency and accuracy by DNA polymerases. The replicative polymerase in E. coli is DNA Pol III, which is a complex of 10 different subunits that coordinates simultaneous replication on the leading and lagging strands. The 1160-residue Pol III alpha subunit is responsible for the polymerase activity and copies DNA accurately, making one error per 10 5 nucleotide incorporations. The goal of this research is to determine the residues that contribute to the activity of the polymerase subunit. Homology modeling and the computational methods of THEMATICS and POOL were used to predict functionally important amino acid residues through their computed chemical properties. Site-directed mutagenesis and biochemical assays were used to validate these predictions. Primer extension, steady-state single-nucleotide incorporation kinetics, and thermal denaturation assays were performed to understand the contribution of these residues to the function of the polymerase. This work shows that the top 15 residues predicted by POOL, a set that includes the three previously known catalytic aspartate residues, seven remote residues, plus five previously unexplored first-layer residues, are important for function. Six previously unidentified residues, R362, D405, K553, Y686, E688, and H760, are each essential to Pol III activity; three additional residues, Y340, R390, and K758, play important roles in activity.

  7. Engajamento cívico e escolaridade superior: as eleições de 2014 e o comportamento político dos brasileiros

    Directory of Open Access Journals (Sweden)

    André Luiz Vieira Dias

    2015-12-01

    Full Text Available Resumo O artigo tem como objetivo analisar a influência da escolaridade sobre o comportamento político dos brasileiros. Supõe-se que a escolaridade é capaz de despertar um comportamento mais interessado, participativo e coerente, portanto, engajado, dos cidadãos. Nesse sentido, busca-se verificar (i se os cidadãos brasileiros são informados e interessados por política; (ii motivados em participar das eleições e outras atividades políticas e (iii sua avaliação e satisfação em relação ao processo democrático. Destaca-se o comportamento daqueles que possuem o ensino superior completo, em comparação aos demais níveis de escolaridade, no intuito de identificar padrões comportamentais distintos. A partir dos dados obtidos pelo ESEB 2014, além da análise descritiva dos dados, aplicou-se o modelo de regressão logística, relacionando a variável escolaridade superior a outras variáveis agrupadas em quatro categorias: (a aspectos socioeconômicos; (b informação e interesse por política; (c participação nas eleições, em atividades políticas tradicionais e em outras atividades políticas; (d avaliação e satisfação política. Dessa maneira, verifica-se (i que os brasileiros são pouco ou nada interessados por política; (ii participam das eleições mas não das atividades tradicionais e novas formas de engajamento político; (iii apoiam e estão razoavelmente satisfeitos com a democracia. Em relação aos mais escolarizados, temos o predomínio de mulheres, um público mais jovem e com renda familiar sutilmente superior à dos menos escolarizados. Aqueles que possuem o ensino superior completo tendem a se comportar de forma distinta: são levemente mais informados e interessados por política; apesar dos baixos percentuais encontrados, são os que mais participam das atividades políticas em geral; são os que mais apoiam a democracia, porém os mais insatisfeitos com o seu funcionamento. Esse estudo nos permite

  8. Cervantes y la Corte: Lecturas biográficas, patrocinio e interpretaciones políticas

    Directory of Open Access Journals (Sweden)

    Mario CRESPO LÓPEZ

    2009-12-01

    Full Text Available RESUMEN: La vida y la obra de Miguel de Cervantes contienen datos de indudable interés para el estudio de la política de la Edad Moderna y de las conexiones áulicas entre el creador literato y los grupos de poder de su época. Lo que conocemos de la biografía de Cervantes, sus inquietudes vitales, los argumentos de sus obras y los misterios que rodearon su mecenazgo y participación en la «república de las letras» se relacionan no sólo con el contexto político de desencanto tras la grandeza imperial de Felipe II sino también con la relación con personajes concretos de la Corte de Felipe III y con algunos acontecimientos, como la expulsión de los moriscos, que marcaron tanto el reinado como la propia obra cervantina. Lo cierto es que no existen demasiadas fuentes a partir de las cuales puedan estudiarse estas conexiones, pero es indudable que el creador está unido a una época en la que debe sobrevivir y a la que debe gran parte de sus preocupaciones literarias. En este trabajo se esbozan algunas conexiones políticas de Cervantes, recuperando parte de la bibliografía de los llamados «cervantistas» decimonónicos.ABSTRACT: Miguel de Cervantes' life and work contain interesting data to study the Modern Age politics and courts connections between the writer and the power ful groups at that time. Cervantes biography, his vital inquietudes, the plot in his works, the misteries of his literary sustenance and his participation in the «república de las letras» can be relationed not only with the political context of disenchantment after Philip II imperial grandeeship but also with the relation with concrete persons in the Court of Philip III and with some events, like Morisco's expulsion, that determine the reign and own Cervantes' work. There are not enough data to study those connections, but it is indubitable that the writer lives in a time where he must survive and where he shows his literary worries. This work sketches some

  9. Raymond Aron ante el maquiavelismo político

    Directory of Open Access Journals (Sweden)

    Molina Cano, Jerónimo

    2008-08-01

    Full Text Available It is a simplification of Raymond Aron´s thought to consider him as a mere neo-liberal political thinker. The French sociologist, a well-known political realist, took part in the famous controversy about Machiavellianism that was Aron´s intellectual watershed from the Forties. Starting from that controversy the aim of the present paper is to inquire into his contribution to the so-called “moderate Machiavellianism”, whose Arcanum is that it is not always possible to choose the optimal means to face the right political action. Machiavellianism is not a merely scientific or epistemological question, but something that deeply determines all political actions.

    La presentación de Raymond Aron como un escritor político neoliberal constituye una simplificación de su pensamiento. El sociólogo francés, que pertenece a la tradición del realismo político, tuvo una destacada intervención en la famosa polémica sobre el maquiavelismo que marcó un punto de inflexión en su pensamiento. Este artículo examina su contribución a esa inagotable polémica intelectual desde los años 40 y constata, finalmente, su encuadramiento en el denominado “maquiavelismo moderado”, cuyo arcano político es que, en política, no siempre se pueden elegir los medios de la acción. El maquiavelismo no es, en este sentido, un asunto científico o epistemológico, sino que determina profundamente toda acción política.

  10. Cobalt(III) complex

    Indian Academy of Sciences (India)

    Administrator

    e, 40 µM complex, 10 hrs after dissolution, f, 40 µM complex, after irradiation dose 15 Gy. and H-atoms result in reduction of Co(III) to Co. (II). 6. It is interesting to see in complex containing multiple ligands what is the fate of electron adduct species formed by electron addition. Reduction to. Co(II) and intramolecular transfer ...

  11. Calculus III essentials

    CERN Document Server

    REA, Editors of

    2012-01-01

    REA's Essentials provide quick and easy access to critical information in a variety of different fields, ranging from the most basic to the most advanced. As its name implies, these concise, comprehensive study guides summarize the essentials of the field covered. Essentials are helpful when preparing for exams, doing homework and will remain a lasting reference source for students, teachers, and professionals. Calculus III includes vector analysis, real valued functions, partial differentiation, multiple integrations, vector fields, and infinite series.

  12. Orthoretroviral-like prototype foamy virus gag-pol expression is compatible with viral replication

    Directory of Open Access Journals (Sweden)

    Reh Juliane

    2011-08-01

    Full Text Available Abstract Background Foamy viruses (FVs unlike orthoretroviruses express Pol as a separate precursor protein and not as a Gag-Pol fusion protein. A unique packaging strategy, involving recognition of briding viral RNA by both Pol precursor and Gag as well as potential Gag-Pol protein interactions, ensures Pol particle encapsidation. Results Several Prototype FV (PFV Gag-Pol fusion protein constructs were generated to examine whether PFV replication is compatible with an orthoretroviral-like Pol expression. During their analysis, non-particle-associated secreted Pol precursor protein was discovered in extracellular wild type PFV particle preparations of different origin, copurifying in simple virion enrichment protocols. Different analysis methods suggest that extracellular wild type PFV particles contain predominantly mature p85PR-RT and p40IN Pol subunits. Characterization of various PFV Gag-Pol fusion constructs revealed that PFV Pol expression in an orthoretroviral manner is compatible with PFV replication as long as a proteolytic processing between Gag and Pol proteins is possible. PFV Gag-Pol translation by a HIV-1 like ribosomal frameshift signal resulted in production of replication-competent virions, although cell- and particle-associated Pol levels were reduced in comparison to wild type. In-frame fusion of PFV Gag and Pol ORFs led to increased cellular Pol levels, but particle incorporation was only marginally elevated. Unlike that reported for similar orthoretroviral constructs, a full-length in-frame PFV Gag-Pol fusion construct showed wildtype-like particle release and infectivity characteristics. In contrast, in-frame PFV Gag-Pol fusion with C-terminal Gag ORF truncations or non-removable Gag peptide addition to Pol displayed wildtype particle release, but reduced particle infectivity. PFV Gag-Pol precursor fusion proteins with inactivated protease were highly deficient in regular particle release, although coexpression of p71Gag

  13. Infinite Particle Systems: Complex Systems III

    Directory of Open Access Journals (Sweden)

    Editorial Board

    2008-06-01

    Full Text Available In the years 2002-2005, a group of German and Polish mathematicians worked under a DFG research project No 436 POL 113/98/0-1 entitled "Methods of stochastic analysis in the theory of collective phenomena: Gibbs states and statistical hydrodynamics". The results of their study were summarized at the German-Polish conference, which took place in Poland in October 2005. The venue of the conference was Kazimierz Dolny upon Vistula - a lovely town and a popular place for various cultural, scientific, and even political events of an international significance. The conference was also attended by scientists from France, Italy, Portugal, UK, Ukraine, and USA, which predetermined its international character. Since that time, the conference, entitled "Infinite Particle Systems: Complex Systems" has become an annual international event, attended by leading scientists from Germany, Poland and many other countries. The present volume of the "Condensed Matter Physics" contains proceedings of the conference "Infinite Particle Systems: Complex Systems III", which took place in June 2007.

  14. Por que rir da Filosofia Política?: Abertura

    Directory of Open Access Journals (Sweden)

    Lessa Renato

    1998-01-01

    Full Text Available As diferentes intervenções que compuseram este debate têm como referência comum uma reflexão sobre os lugares da Filosofia Política na tradição disciplinar que designamos como Ciência Política. Recusando a perspectiva que dissocia a Filosofia Política da dimensão empiricamente orientada da disciplina, os argumentos apresentados destacam o papel de fertilização de formas de vida cumprido pela primeira. A agenda dos pesquisadores devotados à boa faina da investigação empírica foi, e segue sendo, em grande medida definida e configurada por exercícios prévios de invenção social e política e por decisões de ordem ontológica, epistemológica e retórica. Neste sentido, a distinção entre ciência e filosofia é, além de obscurantista e marcada por enorme otimismo epistemológico, sintoma de um desconhecimento forte com relação à história do conhecimento político.

  15. Polímeros como modificadores asfálticos

    Directory of Open Access Journals (Sweden)

    Letícia SocaI da Silva

    2009-12-01

    Full Text Available

    Neste trabalho foram preparadas diferentes misturas de ligante asfáltico modificado com polímeros. Foram utilizadas quatro amostras de polímeros, sendo duas de SBS, uma de SEBS e uma de Polietileno Modificado, as quais foram caracterizadas quanto a sua estrutura e propriedades térmicas através de técnicas de Ressonância Magnética Nuclear (RMN, Cromatografia por Permeação a Gel (GPC, Análise Termogravimétrica (TGA e Calorimetria Diferencial de Varredura (DSC. O ligante modificado foi avaliado quanto a sua viscosidade, retorno elástico, comportamento reológico e estabilidade à estocagem. Foram correlacionadas a estrutura química e as transições térmicas dos polímeros com as características do ligante modificado. Os polímeros do tipo SBS foram os melhores modificadores de asfalto, quando comparado com os demais polímeros avaliados.

  16. El Desequilibrio de lo Político en Hegel

    Directory of Open Access Journals (Sweden)

    Juan Ignacio Arias Krause

    2016-07-01

    Full Text Available El problema político de la sociedad civil se fundamenta en su doble forma de manifestarse: por un lado, en ella se revela el carácter externo u objetivo del Estado y, por otro, produce una escisión al interior del mismo Estado. Tal problema que aparece como contradictorio (el ser exterior e interior a la vez es presentado en el pensamiento político de Hegel, en vistas a superar las dicotomías que en la teoría política moderna aparecían como insalvables. Esta superación Hegel la realiza mediante una interiorización de aquellos elementos que anteriormente habían sido marginados de la política, como son lo natural y el dominio privado, los que emergerían en la sociedad civil. En este artículo se exponen estos elementos en vistas a mostrar cómo es en este movimiento de interiorización y exteriorización de ellos donde se posibilita el acontecer de lo político.

  17. Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol

    Directory of Open Access Journals (Sweden)

    Shalak V. F.

    2011-10-01

    Full Text Available Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods. Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associates with the Pol domain of GagPol. Conclusions. A model of the assembly of the LysRS:tRNA3Lys:GagPol packaging complex is proposed.

  18. Salazarismo y política exterior

    Directory of Open Access Journals (Sweden)

    Juan Carlos JIMÉNEZ REDONDO

    2010-02-01

    Full Text Available RESUMEN: El sistema internacional jugó para la Portugal de Salazar un papel esencial al actuar como fuente privilegiada de la que extraer los recursos materiales (económicos, tecnológicos o financieros e inmateriales (factores de legitimación que garantizaron el programa de reforma política de la dictadura y que permitieron al país desempeñar un papel internacional desproporcionadamente relevante si consideramos su condición de pequeña potencia periférica europea. La novedad del salazarismo respecto a períodos anteriores consistió en la adecuación de esas necesidades de apertura al exterior con una fuerte percepción de los intereses nacionales, dentro de una estrategia pragmática que le permitió acomodarse a la acelerada evolución del sistema internacional y, sobre todo, al cambio que se produjo en el sistema internacional de acuerdo a la dinámica de la Guerra Fría y sus distintas fases. Palabras clave: Salazarismo, OTAN, EFTA, Botelho Moniz, Guerra colonial, descolonización. ABSTRACT: The international system played for the Portugal of Salazar an essential role when acting like privileged source of which to extract the material resources (economic, technological or financial and immaterial (legitimation factors that guaranteed the program of political reform of the dictatorship and that allowed the country to play very out of proportion excellent a role international if we considered its condition of small European peripheral power. The newness of the salazarism with respect to previous periods consisted of the adjustment of those necessities of opening to the outside with a strong perception of the national interests, within a pragmatic strategy that allowed to comply him to the accelerated evolution of the international system and, mainly, to the change that took place in the international system according to the dynamics of the cold war and its different phases. Keywords: Salazarism, NATO, EFTA, Botelho Moniz, Colonial

  19. ¿Arte y política o política en el arte?

    Directory of Open Access Journals (Sweden)

    Carlos Araque Osorio

    2014-12-01

    Full Text Available Este es nuestro segundo número dedicado al tema “Arte y Política” y valdría la pena preguntarnos¿por qué dos números sobre un tema que está en boca de todo el mundo?-Académicamente se trata a diario y aparece recurrentemente en periódicos, revistas ynoticieros-. Lo cierto es, que el arte genera necesidades, conforma comunidades y sociedades,moldea conductas, da status, constituye identidad e incluso destaca virtudes y pudepropiciar que algunas verdades no tan reales en el imaginario colectivo, se trasformen eninvenciones o quimeras.También, es cierto que la influencia de los medios masivos de comunicación está presenteen el gusto que demuestran las comunidades por el arte y los artistas. Hoy en día por ejemplo:el artista, el título de la obra y la justificación o sustentación que se haga de ella, puedenser más importantes que la obra. Si a esto agregamos que es en las oficinas es donde losmass media deciden sobre que arte promocionar e impulsar y que obras desconocer oignorar, tendríamos que afirmar que en la contemporaneidad el vínculo entre arte y políticava más allá de una relación formal y se convierte en una forma de poder.

  20. Arabidopsis Pol II-Dependent in Vitro Transcription System Reveals Role of Chromatin for Light-Inducible rbcS Gene Transcription1

    Science.gov (United States)

    Ido, Ayaka; Iwata, Shinya; Iwata, Yuka; Igarashi, Hisako; Hamada, Takahiro; Sonobe, Seiji; Sugiura, Masahiro; Yukawa, Yasushi

    2016-01-01

    In vitro transcription is an essential tool to study the molecular mechanisms of transcription. For over a decade, we have developed an in vitro transcription system from tobacco (Nicotiana tabacum)-cultured cells (BY-2), and this system supported the basic activities of the three RNA polymerases (Pol I, Pol II, and Pol III). However, it was not suitable to study photosynthetic genes, because BY-2 cells have lost their photosynthetic activity. Therefore, Arabidopsis (Arabidopsis thaliana) in vitro transcription systems were developed from green and etiolated suspension cells. Sufficient in vitro Pol II activity was detected after the minor modification of the nuclear soluble extracts preparation method; removal of vacuoles from protoplasts and L-ascorbic acid supplementation in the extraction buffer were particularly effective. Surprisingly, all four Arabidopsis Rubisco small subunit (rbcS-1A, rbcS-1B, rbcS-2B, and rbcS-3B) gene members were in vitro transcribed from the naked DNA templates without any light-dependent manner. However, clear light-inducible transcriptions were observed using chromatin template of rbcS-1A gene, which was prepared with a human nucleosome assembly protein 1 (hNAP1) and HeLa histones. This suggested that a key determinant of light-dependency through the rbcS gene transcription was a higher order of DNA structure (i.e. chromatin). PMID:26662274

  1. Asia Central en la política exterior rusa

    Directory of Open Access Journals (Sweden)

    Javier Morales

    2012-01-01

    Full Text Available Las políticas rusas hacia Asia Central han estado condicionadas por la herencia histórica anterior a la disolución de la URSS, pero también por los cambios en el sistema internacional y en la propia Rusia acaecidos desde entonces. ¿En qué medida la actuación de Rusia ha sido coherente y estable, basada en una estrategia para Asia Central fundamentada en los intereses nacionales? En este artículo examinamos los fundamentos de la política exterior rusa en relación con Asia Central, dividiéndola en tres apartados: político, económico y de seguridad.

  2. Ajuste macro, política monetaria y empleo

    Directory of Open Access Journals (Sweden)

    Jorge Iván González

    2000-04-01

    Full Text Available El artículo muestra que la política monetaria restrictiva ha sido uno de los factores que más ha incidido en el crecimiento del desempleo en Colombia durante la segunda mitad de los años noventa. La tasa de sacrificio de la política monetaria no ha sido cero, porque el costo de reducir la inflación ha sido muy elevado. La responsabilidad que tiene el manejo de la política monetaria en la crisis del sector real se hace más visible si se tiene en cuenta que no hay evidencia de que la menor demanda de trabajo esté asociada a los costos laborales y a las inflexibilidades del mercado laboral.

  3. Elecciones, Jóvenes y Política

    Directory of Open Access Journals (Sweden)

    Anna María Fernández Poncela

    1999-01-01

    Full Text Available Este texto muestra el papel de la juventud en un posible cambio político, especialmente en el espacio elec toral. Se centra, en concreto, en la postura político - elec toral de los jóvenes en nuestros días y de cara al fu turo cercano; por una parte, comparada con otros grupos de edad; por otra, específicamente en tre los universitarios, y finalmente intentando vislumbrar los escenarios del porvenir al respecto. Para ello se emplearán datos de una encuesta nacional, encuestas universitarias y sondeos de opinión en torno a la convocatoria de 1997, sobre todo en los resultados de las preguntas sobre la simpatía política y preferencias electorales, a quiénes se votó y a quiénes se votará.

  4. Naturaleza humana y política en Denis Diderot

    Directory of Open Access Journals (Sweden)

    Pablo Scotto Benito

    2015-02-01

    Full Text Available El presente trabajo se propone relacionar la concepción que Diderot tiene del ser humano con sus ideas políticas. A pesar de que no se pueda hallar en la obra del “filósofo” un tratado sistemático en el que el estudio de la naturaleza humana sirva como fundamentación de un determinado sistema político, la epistemología empirista y el monismo materialista diderotianos, con la consecuente revalorización de las pasiones corporales, conducen a una moral y una política universalistas, acordes con la naturaleza humana y centradas en la felicidad de los individuos.

  5. La política como espectáculo

    Directory of Open Access Journals (Sweden)

    Juan E. Fernández

    2015-01-01

    Full Text Available La política siempre estuvo ligada al espectáculo, la pompa y el teatro. La antigua oratoria imperial, eclesiástica y solemne; los debates cargados de ironías en asambleas y parlamentos; y los actuales espectáculos políticos televisados difieren más en la forma y el alcance (curiosamente más democrático que en su ausencia comunicacional. El autor piensa, que hay mucho de siniestro y peligroso en la trivialización del camino al poder cuando lo que está en juego parece ser la vida misma.

  6. La ciencia de la política.

    OpenAIRE

    Bolívar Meza, Rosendo

    2007-01-01

    Presentación. Ramos Jiménez, Alfredo Los círculos bolivarianos. El mito de la unidad del pueblo. Arenas, Nelly y Gómez Calcaño, Luis Lo massmediático y las sociedades contemporáneas. Un acercamiento a la relación medios-democracia. Briceño Romero, Ysabel La economía ambiental y la política ambiental. Caraballo, Leonardo Javier Veinte años de democracia en la Argentina. ¿Qué democracia? Kers, Mercedes y Leiras, Santiago C. La ciencia de la política. Bolívar ...

  7. La agricultura peruana: problemas, potencialidades y políticas

    OpenAIRE

    Adolfo Figueroa

    1986-01-01

    El presente trabajo tiene por objeto presentar, primero, los problemas estructurales de la agricultura peruana, luego sus potencialidades y, finalmente, los elementos para el diseño de políticas económicas. Debo admitir que este objetivo es muy ambicioso para ser desarrollado en un artículo y por un investigador individual. Por ello espero que el artículo logre, más bien, ordenar los elementos básicos para la discusión de la política económica dirigida al desarrollo agrícola del Perú....

  8. Intelectuais, cultura política e ditadura no Brasil

    Directory of Open Access Journals (Sweden)

    Michel Goulart da Silva

    2013-05-01

    Full Text Available Discute-se neste artigo a produção de uma cultura política compartilhada por intelectuais no contexto da ditadura civil-militar, que governou o Brasil entre os anos de 1964 e 1985. Apresenta-se numa primeira parte a relação estabelecida pelos intelectuais com o regime autoritário, principalmente a partir das reflexões realizadas pelo sociólogo Florestan Fernandes durante a ditadura. Numa segunda parte trabalha-se a noção de cultura política conservadora, compartilhada por uma parcela dos intelectuais brasileiros.

  9. O desafio político do desenvolvimento sustentado

    OpenAIRE

    Guimarães,Roberto P.

    1995-01-01

    A unanimidade que hoje parece haver em favor do desenvolvimento sustentado contrasta com a ausência de iniciativas políticas significativas para transformar as instituições econômicas, sociais e políticas que estão comprometidas com o estilo vigente de desenvolvimento. Em particular, há uma patente contradição entre o discurso antiestatista hegemônico e as exigências do desenvolvimento sustentado, que incluem um Estado ainda mais forte, em suas capacidades reguladoras e de planificação, que o...

  10. Responsabilidad social corporativa y conexiones políticas

    OpenAIRE

    Ceballos Gallego, Nicolás Bernardo

    2017-01-01

    La inclusión de políticas de Responsabilidad Social en la actividad productiva es una estrategia que en la actualidad se ve asociada al buen desempeño empresarial. Paralelo a esto, se lleva a cabo un ejercicio de respaldo a causas políticas que define el comportamiento de las compañías en términos de gobierno corporativo. Se encuentra una relación positiva y significativa entre ambos conceptos sugiriendo una relación complementaria entre ambos y asociada a causas filantrópicas más allá de lo...

  11. La justicia como problema político. Del constructivismo moral kantiano al constructivismo político rawlsiano

    Directory of Open Access Journals (Sweden)

    Jefferson Jaramillo Marín

    2009-01-01

    Full Text Available El artículo expone la justicia como un problema político desde la perspectiva del filósofo John Rawls. En su desarrollo se señalan las implicaciones del constructivismo kantiano en el constructivismo político rawlsiano. Se discute cómo la conexión entre estos dos tipos de constructivismo, proporciona un procedimiento de construcción, en el que agentes racionalmente autónomos, sujetos a condiciones razonables, elaboran acuerdos sobre principios públicos de justicia para lograr un sistema justo de cooperación que trascienda generacionalmente. Se concluye, mostrando las limitaciones y alcances de esta propuesta en el pensamiento filosófico y político contemporáneo.

  12. Roles of Saccharomyces cerevisiae DNA polymerases Polη and Polζ in response to irradiation by simulated sunlight

    Science.gov (United States)

    Kozmin, Stanislav G.; Pavlov, Youri I.; Kunkel, Thomas A.; Sage, Evelyne

    2003-01-01

    Sunlight causes lesions in DNA that if unrepaired and inaccurately replicated by DNA polymerases yield mutations that result in skin cancer in humans. Two enzymes involved in translesion synthesis (TLS) of UV-induced photolesions are DNA polymerase η (Polη) and polymerase ζ (Polζ), encoded by the RAD30A and REV3 genes, respectively. Previous studies have investigated the TLS roles of these polymerases in human and yeast cells irradiated with monochromatic, short wavelength UVC radiation (254 nm). However, less is known about cellular responses to solar radiation, which is of higher and mixed wavelengths (310–1100 nm) and produces a different spectrum of DNA lesions, including Dewar photoproducts and oxidative lesions. Here we report on the comparative cytotoxic and mutagenic effects of simulated sunlight (SSL) and UVC radiation on yeast wild-type, rad30Δ, rev3Δ and rev3Δ rad30Δ strains. The results with SSL support several previous interpretations on the roles of these two polymerases in TLS of photodimers and (6–4) photoproducts derived from studies with UVC. They further suggest that Polη participates in the non-mutagenic bypass of SSL-dependent cytosine-containing Dewar photoproducts and 8-oxoguanine, while Polζ is mainly responsible for the mutagenic bypass of all types of Dewar photoproducts. They also suggest that in the absence of Polζ, Polη contributes to UVC- and SSL-induced mutagenesis, possibly by the bypass of photodimers containing deaminated cytosine. PMID:12888515

  13. As assimetrias entre as políticas setoriais e a política de planejamento regional no Brasil

    Directory of Open Access Journals (Sweden)

    João Mendes da Rocha Neto

    2011-12-01

    Full Text Available O modo de conduzir a formulação das políticas de desenvolvimento regional tem se constituído em ampla arena de embates acadêmicos e técnicos e levado a reflexões sobre os rumos das múltiplas políticas que acompanham esse processo de planejamento regional. Isso, em parte, decorre do processo de globalização e das políticas neoliberais que o acompanham, as quais possuem um forte apelo à competitividade. Essa estratégia de buscar espaços "privilegiados" se fez presente de uma forma intensa em alguns setores produtivos, que, ao usar o espaço como mercadoria, utilizam seu conjunto de atributos (naturais e artificiais para realizarem-se e reproduzirem-se como parte do sistema. Assim, a proposta de estudo pretende responder a questão: em que medida as políticas públicas setoriais têm dialogado com as políticas de planejamento e desenvolvimento regional no âmbito do governo federal? No caso das políticas de planejamento regional, o recorte espacial é visto como um instrumento que, ao ser aplicado, pode se mostrar capaz de viabilizar a integração de ações multissetorializadas, o que em certa dimensão apontaria para uma maior eficiência do Estado na busca por restabelecer o equilíbrio esgarçado, tornando mais eficiente o planejamento.

  14. File list: Pol.CDV.05.AllAg.Cardiomyocytes [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.05.AllAg.Cardiomyocytes mm9 RNA polymerase Cardiovascular Cardiomyocytes ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.05.AllAg.Cardiomyocytes.bed ...

  15. File list: Pol.CDV.20.AllAg.Cardiomyocytes [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.20.AllAg.Cardiomyocytes mm9 RNA polymerase Cardiovascular Cardiomyocytes ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.20.AllAg.Cardiomyocytes.bed ...

  16. File list: Pol.CDV.10.AllAg.Cardiomyocytes [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.10.AllAg.Cardiomyocytes mm9 RNA polymerase Cardiovascular Cardiomyocytes ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.10.AllAg.Cardiomyocytes.bed ...

  17. File list: Pol.CDV.50.AllAg.Cardiomyocytes [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.50.AllAg.Cardiomyocytes mm9 RNA polymerase Cardiovascular Cardiomyocytes ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.50.AllAg.Cardiomyocytes.bed ...

  18. File list: Pol.Emb.05.AllAg.Embryonic_palates [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.05.AllAg.Embryonic_palates mm9 RNA polymerase Embryo Embryonic palates http...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Emb.05.AllAg.Embryonic_palates.bed ...

  19. File list: Pol.Emb.20.AllAg.Embryonic_palates [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.20.AllAg.Embryonic_palates mm9 RNA polymerase Embryo Embryonic palates http...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Emb.20.AllAg.Embryonic_palates.bed ...

  20. File list: Pol.Kid.20.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Kid.20.AllAg.Nephrectomy_sample hg19 RNA polymerase Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Kid.20.AllAg.Nephrectomy_sample.bed ...

  1. File list: Pol.Kid.10.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Kid.10.AllAg.Nephrectomy_sample hg19 RNA polymerase Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Kid.10.AllAg.Nephrectomy_sample.bed ...

  2. File list: Pol.Kid.05.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Kid.05.AllAg.Nephrectomy_sample hg19 RNA polymerase Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Kid.05.AllAg.Nephrectomy_sample.bed ...

  3. File list: Pol.Bld.20.AllAg.Polymorphonuclear_leukocytes [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bld.20.AllAg.Polymorphonuclear_leukocytes hg19 RNA polymerase Blood Polymorphonuclear... leukocytes http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Bld.20.AllAg.Polymorphonuclear_leukocytes.bed ...

  4. File list: Pol.Bld.50.AllAg.Polymorphonuclear_leukocytes [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bld.50.AllAg.Polymorphonuclear_leukocytes hg19 RNA polymerase Blood Polymorphonuclear... leukocytes http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Bld.50.AllAg.Polymorphonuclear_leukocytes.bed ...

  5. File list: Pol.Bld.05.AllAg.Polymorphonuclear_leukocytes [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bld.05.AllAg.Polymorphonuclear_leukocytes hg19 RNA polymerase Blood Polymorphonuclear... leukocytes http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Bld.05.AllAg.Polymorphonuclear_leukocytes.bed ...

  6. File list: Pol.Lng.10.AllAg.Lung_adenocarcinoma [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Lng.10.AllAg.Lung_adenocarcinoma mm9 RNA polymerase Lung Lung adenocarcinoma ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Lng.10.AllAg.Lung_adenocarcinoma.bed ...

  7. File list: Pol.Emb.20.AllAg.Embryonic_pancreas [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.20.AllAg.Embryonic_pancreas mm9 RNA polymerase Embryo Embryonic pancreas ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Emb.20.AllAg.Embryonic_pancreas.bed ...

  8. File list: Pol.Emb.10.AllAg.Embryonic_pancreas [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.10.AllAg.Embryonic_pancreas mm9 RNA polymerase Embryo Embryonic pancreas ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Emb.10.AllAg.Embryonic_pancreas.bed ...

  9. File list: Pol.Emb.05.AllAg.Embryonic_pancreas [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.05.AllAg.Embryonic_pancreas mm9 RNA polymerase Embryo Embryonic pancreas ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Emb.05.AllAg.Embryonic_pancreas.bed ...

  10. File list: Pol.Adl.05.AllAg.Octopaminergic_neurons [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.05.AllAg.Octopaminergic_neurons dm3 RNA polymerase Adult Octopaminergic neurons... http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Adl.05.AllAg.Octopaminergic_neurons.bed ...

  11. File list: Pol.Adl.20.AllAg.Octopaminergic_neurons [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.20.AllAg.Octopaminergic_neurons dm3 RNA polymerase Adult Octopaminergic neurons... http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Adl.20.AllAg.Octopaminergic_neurons.bed ...

  12. File list: Pol.Adl.50.AllAg.Octopaminergic_neurons [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.50.AllAg.Octopaminergic_neurons dm3 RNA polymerase Adult Octopaminergic neurons... http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Adl.50.AllAg.Octopaminergic_neurons.bed ...

  13. File list: Pol.Adl.10.AllAg.Octopaminergic_neurons [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.10.AllAg.Octopaminergic_neurons dm3 RNA polymerase Adult Octopaminergic neurons... http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Adl.10.AllAg.Octopaminergic_neurons.bed ...

  14. File list: Pol.CDV.10.AllAg.Atrioventicular_canals [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.10.AllAg.Atrioventicular_canals mm9 RNA polymerase Cardiovascular Atrioventicular canals... http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.10.AllAg.Atrioventicular_canals.bed ...

  15. File list: Pol.CDV.05.AllAg.Atrioventicular_canals [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.05.AllAg.Atrioventicular_canals mm9 RNA polymerase Cardiovascular Atrioventicular canals... http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.05.AllAg.Atrioventicular_canals.bed ...

  16. File list: Pol.CDV.50.AllAg.Atrioventicular_canals [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.50.AllAg.Atrioventicular_canals mm9 RNA polymerase Cardiovascular Atrioventicular canals... http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.50.AllAg.Atrioventicular_canals.bed ...

  17. File list: Pol.CDV.20.AllAg.Atrioventicular_canals [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.20.AllAg.Atrioventicular_canals mm9 RNA polymerase Cardiovascular Atrioventicular canals... http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.20.AllAg.Atrioventicular_canals.bed ...

  18. La justicia como problema político. Del constructivismo moral kantiano al constructivismo político rawlsiano

    OpenAIRE

    Jefferson Jaramillo Marín; Yesid Echeverry Enciso

    2009-01-01

    El artículo expone la justicia como un problema político desde la perspectiva del filósofo John Rawls. En su desarrollo se señalan las implicaciones del constructivismo kantiano en el constructivismo político rawlsiano. Se discute cómo la conexión entre estos dos tipos de constructivismo, proporciona un procedimiento de construcción, en el que agentes racionalmente autónomos, sujetos a condiciones razonables, elaboran acuerdos sobre principios públicos de justicia para lograr un sistema justo...

  19. La escisión entre Ciencia Política y realidad política: el caso de la Seguridad Democrática

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Ramírez González

    2014-12-01

    Full Text Available La política de seguridad democrática, adoptada por el Presidente Álvaro Uribe Vélez durante su periodo de gobierno, resultó en un viraje fundamental en los rumbos del país luego del fallido proceso de paz del gobierno Pastrana. En este sentido, el presente trabajo busca demostrar que la Ciencia Política abordó el fenómeno político en contravía con el curso de los hechos, de tal manera que mientras la política de Seguridad Democrática propugnaba por un aumento en la ofensiva militar, la visión desde la Ciencia Política se concentraba en la manera de llegar a unos diálogos de paz o una salida concertada al conflicto. Esto, por tanto, se puede traducir en un rechazo al punto de vista preponderante y a una postura alternativa en el estudio de la realidad política nacional. Para demostrar lo anterior, se realiza una revisión documental aplicada a artículos académicos en cuatro revistas de Ciencia Política del país: Análisis Político, Colombia Internacional, Papel Político y Estudios Políticos durante el periodo de publicación de 2002 a 2012.

  20. A política do ensaio político de catálogo

    Directory of Open Access Journals (Sweden)

    Julian Stallabrass

    2010-01-01

    Full Text Available Este texto foi originalmente publicado no livro Villa Lituania, que aborda o trabalho homônimo, apresentado pela dupla de artistas lituanos Nomeda & Gediminas Urbonas na 52ª Bienal de Veneza, em 2007. O título designa o edifício que até 1940 sediou, em Roma, a primeira embaixada da república independente da Lituania, mantido sob jurisdição das autoridades russas mesmo após a queda do bloco soviético, em 1989, e considerado, pela ex-república soviética, território nacional ocupado. O trabalho implicou na construção de um pombal na Villa Lituania e numa série de ações de conotação política e social em Veneza, Roma e Vilna, cidade onde a dupla reside. Os artistas são conhecidos internacionalmente pela dimensão política e social que pretendem infundir a suas ações. Não obstante a forte carga ideológica que envolveu a intervenção de Nomeda & Gediminas Urbonas na Bienal de Veneza, neste ensaio o autor, tributário da tradição marxista, demonstra notável ceticismo em relação à potência crítica do discurso marxista evocado em parte significativa da arte contemporânea.This text has been originally published in the book Villa Lituania, which approaches the homonymous work presented by the lithuanian artists Nomeda & Gediminas Urbonas, at the 52ª Venice Biennial, in 2007. The title is the name of the building used until 1940 as the seat for the first independent Lithuania Republic's embassy, in Rome, nevertheless kept under the jurisdiction of Russian authorities since the fall of the soviet bloc, in 1989, and considered, by the ex-soviet Republic, national territory under foreign occupation. The work involved the creation of a pigeon loft at Villa Lituania building, as well as a series of actions targeted at social and politic issues in Venice, Rome and Vilnius, where the tandem lives. Socially and politically addressed actions have made the artists internationally known. Notwithstanding the strong ideological drive

  1. Construtivismo Político: Além do Realismo e Antirrealismo

    Directory of Open Access Journals (Sweden)

    Denis Coitinho Silveira

    2011-12-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2011v10n1p1 O artigo tem o objetivo de investigar as características centrais do modelo ético coerentista utilizado por Rawls nos textos A Theory of Justice (TJ I, § 4, 9, Political Liberalism (PL III, “Kantian Constructivism in Moral Theory” (KCMT, “Themes in Kant’s Moral Philosophy” (TKMP, Lectures on History of Moral Philosophy (LHMP: 69-83; 235-252, “Reply to Habermas” (RH § 2, The Law of Peoples (LP I, Justice as Fairness: A Restatement (JF I; V, Collected Papers (CP. Especificamente, quero ver como seu construtivismo político oferece uma alternativa ao problema debatido entre realistas e antirrealistas, a saber, se existem fatos morais independente das crenças ou se estes dependem da estrutura mental do agente, sendo uma alternativa tanto ao construtivismo moral kantiano como ao intuicionismo racional. Quero defender que o construtivismo político é um modelo contratualista coerentista holístico e que faz uso de uma ontologia social, conduzindo a um modelo pragmático de justificação em um âmbito público que supera este impasse em ontologia moral. Para tal, inicio com a controvérsia entre realistas e antirrealistas e, após, analiso a teoria rawlseana à luz deste debate. Por fim, procuro estabelecer algumas considerações conclusivas sobre este problema.

  2. I Pol Makkartni v podarok / Aleksei Shipulin ; interv. Jelena Skulskaja

    Index Scriptorium Estoniae

    Shipulin, Aleksei

    2004-01-01

    Moskvas elav Eesti kodanikust dokumentalist teeb Eestis vene telekanalile "Kultura" dokumentaalfilmi Lennart Merist. Režissöör jutustab ka oma varasematest filmidest nagu "Paul McCartney. 72 tundi Venemaal" ("Pol Makkartni. 72 tshassa v Rossii") ja "Serõi voronok. Komu tõ nuzhen?"

  3. Fast Superpixel Segmentation Algorithm for PolSAR Images

    Directory of Open Access Journals (Sweden)

    Zhang Yue

    2017-10-01

    Full Text Available As a pre-processing technique, superpixel segmentation algorithms should be of high computational efficiency, accurate boundary adherence and regular shape in homogeneous regions. A fast superpixel segmentation algorithm based on Iterative Edge Refinement (IER has shown to be applicable on optical images. However, it is difficult to obtain the ideal results when IER is applied directly to PolSAR images due to the speckle noise and small or slim regions in PolSAR images. To address these problems, in this study, the unstable pixel set is initialized as all the pixels in the PolSAR image instead of the initial grid edge pixels. In the local relabeling of the unstable pixels, the fast revised Wishart distance is utilized instead of the Euclidean distance in CIELAB color space. Then, a post-processing procedure based on dissimilarity measure is empolyed to remove isolated small superpixels as well as to retain the strong point targets. Finally, extensive experiments based on a simulated image and a real-world PolSAR image from Airborne Synthetic Aperture Radar (AirSAR are conducted, showing that the proposed algorithm, compared with three state-of-the-art methods, performs better in terms of several commonly used evaluation criteria with high computational efficiency, accurate boundary adherence, and homogeneous regularity.

  4. Políticas en la organización

    Directory of Open Access Journals (Sweden)

    Alvaro Rubio Salas

    1989-04-01

    Full Text Available RESUMEN Toda organización por así decirlo obtiene una serie de políticas que la destacan y la diferencian de las demás, sin embargo se pueden definir en  planes de acción que reflejan e identifican la personalidad y estilo de vida de esta.

  5. Pseudo Class III malocclusion

    Directory of Open Access Journals (Sweden)

    Fadia M. Al-Hummayani

    2016-04-01

    Full Text Available The treatment of deep anterior crossbite is technically challenging due to the difficulty of placing traditional brackets with fixed appliances. This case report represents a none traditional treatment modality to treat deep anterior crossbite in an adult pseudo class III malocclusion complicated by severely retruded, supraerupted upper and lower incisors. Treatment was carried out in 2 phases. Phase I treatment was performed by removable appliance “modified Hawley appliance with inverted labial bow,” some modifications were carried out to it to suit the presented case. Positive overbite and overjet was accomplished in one month, in this phase with minimal forces exerted on the lower incisors. Whereas, phase II treatment was performed with fixed appliances (braces to align teeth and have proper over bite and overjet and to close posterior open bite, this phase was accomplished within 11 month.

  6. Ammonium diphosphitoindate(III

    Directory of Open Access Journals (Sweden)

    Farida Hamchaoui

    2013-04-01

    Full Text Available The crystal structure of the title compound, NH4[In(HPO32], is built up from InIII cations (site symmetry 3m. adopting an octahedral environment and two different phosphite anions (each with site symmetry 3m. exhibiting a triangular–pyramidal geometry. Each InO6 octahedron shares its six apices with hydrogen phosphite groups. Reciprocally, each HPO3 group shares all its O atoms with three different metal cations, leading to [In(HPO32]− layers which propagate in the ab plane. The ammonium cation likewise has site symmetry 3m.. In the structure, the cations are located between the [In(HPO32]− layers of the host framework. The sheets are held together by hydrogen bonds formed between the NH4+ cations and the O atoms of the framework.

  7. Fast ejendom III

    DEFF Research Database (Denmark)

    Munk-Hansen, Carsten

    Bogen er det tredje bind af tre planlagte bind om fast ejendom: I Overdragelsen, II Bolighandlen og III Ejerbeføjelsen. Fremstillingens giver et grundigt overblik over centrale områder af en omfattende regulering af fast ejendom, med angivelse af litteratur, hvor læseren kan søge yderligere...... oplysning. En ejer af fast ejendom er på særdeles mange områder begrænset i sin råden sammenlignet med ejeren af et formuegode i almindelighed. Fremstillingen tager udgangspunkt i ejerens perspektiv (fremfor samfundets eller myndighedernes). Både den privatretlige og offentligretlige regulering behandles......, eksempelvis ejendomsdannelsen, servitutter, naboretten, hævd, zoneinddelingen, den fysiske planlægning, beskyttelse af natur, beskyttelse af kultur, forurening fra fast ejendom, erstatning for forurening, jordforurening, ekspropriation, byggeri og adgang til fast ejendom....

  8. La Defensa, Política de Estado

    Directory of Open Access Journals (Sweden)

    Muñoz-Alonso, Alejandro

    2008-12-01

    Full Text Available The areas of the State Policy are set, specially focussing on the Defense as it is closely related to concepts like National Interest and Anglo-Saxon bipartisan policy. Nevertheless, the political alternation will cause differences. It is also necessary to tell the difference between the State Policy and the Policy carried out by the State. That’s why the democratic Parliaments play a fundamental role in shaping the defence policy. Although the Parliament symbolizes the National Sovereignty there can be and there are actually differences with the Public Opinion. This sort of divorce is not really new as, in Spain, it became evident in a very intensive way on the occasion of decisions such as the Spanish entry into the NATO. A State Policy related to Defense, kept during a long time and resistant to political alternation would certainly be the best instrument to reach a culture of defense.Se establecen los ámbitos de las políticas de estado, con especial énfasis en la Defensa al estar esta vinculada al concepto de interés nacional, y al anglosajón bipartisan policy. No obstante, la alternancia política va a crear discrepancias. Conviene diferenciar la “Política de Estado” y la “Política del Estado”. Por ello, los Parlamentos democráticos juegan un papel fundamental en la conformación de la política de defensa. Pese a que el Parlamento representa a la soberanía nacional pueden producirse, y de hecho se producen, discrepancias con la opinión pública. Este divorcio no es nuevo y en España se ha manifestado de forma muy intensa con ocasión de tomas de decisión como la incorporación a la OTAN. Una política de Estado en defensa, mantenida en el tiempo y resistente a las alternancias políticas sería el instrumento más adecuado para lograr una cultura de defensa.

  9. Políticas públicas: bienestar, reconocimiento, simulacro

    Directory of Open Access Journals (Sweden)

    Rafael Bayce

    2008-01-01

    Full Text Available Las políticas públicas son hijas del crecimiento de los Estados posrrenacentistas y de las necesidades de planificación administrativa de sociedades de masas. Las políticas conservadoras afirman la subsidiariedad del Estado, se centran en la familia y en el temor a la democracia y a los sindicatos. Las liberales se anclan en la corrección de fallas del mercado y de contingencias biológicas. Las socialdemócratas, más ambiciosas, aspiran a una previsión de todas las contingencias y desigualdades ilegítimas, desde un Estado orientador del mercado y no subsidiario. Los Estados de Bienestar surgen del intento de superar recesiones, desempleo y malestar social desde la inversión, hasta deficitaria, de Estados que esperan luego recuperar su inversión. Las nociones de soberanía y de derechos humanos dificultan ese retorno y nutren un crecimiento progresivo de los imaginarios de consumo que erosionan un sistema inicialmente exitoso, desde que no son más inversiones e intervenciones de emergencia sino parte del cálculo de bienestares colectivos e individuales. Son víctimas de su éxito. Pese a que los neoliberales subrayan las insuficiencias teóricas y los fallos históricos de las políticas públicas clásicas, llegados al poder todos las aplican porque la cultura política los supone y enfrentarla sería electoralmente suicida. Hasta los marxistas lo hacen. Sin embargo, la sociedad de consumo y la cultura posmoderna van creando un progresivo hiato entre la moderna oferta tecnoburocrática iluminista de satisfactores, y la más reciente demanda, socialmente mucho más exigente y variada. Deberían disfrutar, por ende, de utilidad marginal político-electoral decreciente. Pero en la medida en que son apreciadas, no por su sustancia material, sino por el ‘reconocimiento’ simbólico que implican, y porque las poblaciones-objetivo han internalizado como legítimas las ofertas iluministas, paterno-maternales, las políticas públicas todav

  10. O contratualismo como método: política, direito e neocontratualismo

    Directory of Open Access Journals (Sweden)

    J. R. N. Chiappin

    2010-02-01

    Full Text Available O objetivo deste artigo é apresentar o contratualismo como método e como o resultado de uma sistematização de outros métodos de construção do conhecimento. Enquanto o contratualismo clássico tem como paradigmas os modelos, geométrico e mecânico, da Física de Galileu e de Descartes - em particular, o modelo de escolha racional com Descartes -, o contratualismo rawlsiano tem como paradigma alguns modelos da microeconomia - em particular, o modelo de equilíbrio geral. O método contratualista tem como projeto a transformação, no século XVII, da política e do Direito em uma disciplina científica, assumindo que, no século XVII: (i o modelo do conhecimento é o modelo geométrico e o conhecimento é conhecimento certo; (ii o modelo de indivíduo admite-o como racional e auto-interessado; (iii há uma teoria racional da ação e decisão humana. Portanto, para viabilizar o projeto de transformar a política e o Direito em ciência, o contratualismo é aqui interpretado não substantivamente, mas instrumentalmente, como método para acessar os fundamentos do mundo político. O método contratualista persegue uma combinação do modelo científico do conhecimento das causas eficientes e de suas relações aos efeitos, com o modelo de conhecimento das causas finais como estudo dos fins ou das características funcionais das coisas. Para interpretarmos o contratualismo como método, admitimos o novo modelo da relação entre conhecimento e tecnologia elaborado pela ciência moderna, que sustenta que o conhecimento da natureza é um conhecimento de conquista e domínio capaz de produzir mecanismos tecnológicos de intervenção na natureza com o objetivo de, conhecendo e manipulando as causas, obter os efeitos desejados.

  11. DnaB gene product-independence of DNA polymerase III-directed repair synthesis in Escherichia coli K-12

    International Nuclear Information System (INIS)

    Billen, D.; Hellermann, G.R.

    1977-01-01

    An investigation has been carried out into the role of dnaB gene product in X-ray-induced repair synthesis carried out by DNA polymerase III in toluene-treated Escherichia coli K-12. A polAl polBlOO dnaB mutant deficient in both DNA polymerase I and II activities was used, and it was shown that the level of X-ray-induced, ATP-dependent, non-conservative DNA synthesis was, unlike semi-conservative DNA synthesis, unaffected by a temperature shift from 30 0 to 42 0 C. The dnaB gene product was not therefore necessary for DNA polymerase III-directed repair synthesis, which occurred in the absence of replicative synthesis. (U.K.)

  12. A interincompreensão gera polêmica: alteridade e heterogeneidade nos discursos das cantoras Anitta e Pitty sobre comportamento sexual feminino

    Directory of Open Access Journals (Sweden)

    Juliane de Araújo Gonzaga

    2017-01-01

    Full Text Available Este artigo se propõe a analisar a situação de polêmica entre as cantoras Anitta e Pitty, durante um programa televiso, em torno do comportamento das mulheres no domínio da sexualidade. Os objetivos específicos são: (i verificar como a alteridade e a heterogeneidade delimitam posições discursivas; (ii descrever a situação de polêmica e a mobilização de simulacros no processo de interincompreensão regrada e (iii analisar a situação de polêmica e demonstrar que a alteridade e a heterogeneidade materializam embates legitimados historicamente no campo da sexualidade. Situado na Análise do Discurso francesa, este trabalho utiliza referencial teórico-metodológico baseado nos pressupostos de Authier-Revuz (1990, 1998, acerca da alteridade e da heterogeneidade enunciativa, e nas considerações de Maingueneau (1993, 2005 sobre o primado do interdiscurso e a polêmica como interincompreensão regrada. O método de análise baseia-se na descrição e interpretação de marcas linguísticas (glosas, pronomes, dêiticos, vocabulário que materializam jogos de alteridade, movimentos de já-ditos e regras sobre o dizível e o indizível no discurso. Por fim, a análise conclui que os enunciados são heterogeneamente marcados e que as posições discursivas produzem situação de polêmica, pois são atravessadas por simulacros, estereótipos e embates históricos entre os gêneros no domínio da sexualidade.

  13. CURRENT SITUATION OF MEDICINE III AND CHALLENGES.

    Science.gov (United States)

    Ferreira, Lydia Masako

    2015-01-01

    tradition and history. For the maintenance and advancement of the area some challenges and goals were established to be developed in the period from 2013 to 2016. Descrever a situação atual da área Medicina III da Capes e detectar os desafios para o próximo quadriênio de avaliação. Os documentos e relatórios de reuniões de área a partir de 2004 até 2013 da Medicina III da Capes foram lidos assim como os relatórios e as fichas de avaliação de cada Programa de Pós Graduação (PPG) da área e a subpágina da área no site da Capes. Os dados referentes ao Processo de Avaliação, à Ficha de Avaliação e aos dados do Corpo Docente, Discente e Produção científica de todos os PPG da Medicina III foram computados e analisados. A partir desses dados foram detectados os desafios da área para o próximo quadriênio (2013-2016). Dentre os 3.806 PPG, a Medicina III apresentava 41 PPG durante última avaliação trienal e evoluiu de 18% para 43% de PPG > conceito muito bom (Triênios 2001-2003 e 2010-2012). A maioria dos PPG se localizavam na Região Sudeste (32), três no Sul e dois no Nordeste. Não havia nenhum PPG no Norte ou Centro-Oeste. Em 2013 e 2014 foram aprovadas quatro mestrados profissionais e um mestrado e doutorado acadêmico. A média de docente permanente no triênio foi de 558 docentes com cerca de três alunos titulados/docente. O número de doutores titulados tem aumentado assim como a razão Doutorado/Mestrado. A proporção de publicações em periódicos de maior impacto (A1, A2, B1 e B2) saltou de 30% para 50% demonstrando resposta positiva da comunidade à política da área. Os desafios detectados foram: diminuir assimetria regional, elevar o número de mestres e doutores de excelência, reavaliação das revistas nacionais, estimular e definir indicadores de internacionalização, inclusão de pós-doutores e definição de seus indicadores, análise da nucleação do PPG, PPG 3x3, inclusão ensino médio e fundamental, mestrado

  14. Type III restriction endonucleases are heterotrimeric: comprising one helicase–nuclease subunit and a dimeric methyltransferase that binds only one specific DNA

    Science.gov (United States)

    Butterer, Annika; Pernstich, Christian; Smith, Rachel M.; Sobott, Frank; Szczelkun, Mark D.; Tóth, Júlia

    2014-01-01

    Fundamental aspects of the biochemistry of Type III restriction endonucleases remain unresolved despite being characterized by numerous research groups in the past decades. One such feature is the subunit stoichiometry of these hetero-oligomeric enzyme complexes, which has important implications for the reaction mechanism. In this study, we present a series of results obtained by native mass spectrometry and size exclusion chromatography with multi-angle light scattering consistent with a 1:2 ratio of Res to Mod subunits in the EcoP15I, EcoPI and PstII complexes as the main holoenzyme species and a 1:1 stoichiometry of specific DNA (sDNA) binding by EcoP15I and EcoPI. Our data are also consistent with a model where ATP hydrolysis activated by recognition site binding leads to release of the enzyme from the site, dissociation from the substrate via a free DNA end and cleavage of the DNA. These results are discussed critically in the light of the published literature, aiming to resolve controversies and discuss consequences in terms of the reaction mechanism. PMID:24510100

  15. Semiconducting III-V compounds

    CERN Document Server

    Hilsum, C; Henisch, Heinz R

    1961-01-01

    Semiconducting III-V Compounds deals with the properties of III-V compounds as a family of semiconducting crystals and relates these compounds to the monatomic semiconductors silicon and germanium. Emphasis is placed on physical processes that are peculiar to III-V compounds, particularly those that combine boron, aluminum, gallium, and indium with phosphorus, arsenic, and antimony (for example, indium antimonide, indium arsenide, gallium antimonide, and gallium arsenide).Comprised of eight chapters, this book begins with an assessment of the crystal structure and binding of III-V compounds, f

  16. Fronteras morales y políticas sexuales: apuntes sobre 'la política LGBT' y el deseo del Estado

    OpenAIRE

    Hernández, Franklin Gil

    2013-01-01

    A partir de un seguimiento de políticas (gubernamentales, de los movimientos sociales y de la vida cotidiana) en la ciudad de Bogotá (Colombia), relacionadas con la ampliación de ciudadanías sexuales, este artículo trata sobre los territorios morales que dichas políticas crean, y sobre los cuerpos que desea el Estado o que reclaman ser deseados por él. Particularmente se analizan la 'política LGBT' y la 'política gay' en un contexto local y su paradójica contribución a la normalización de la ...

  17. Dark matter detection - III

    International Nuclear Information System (INIS)

    Zacek, Viktor

    2015-01-01

    The quest for the missing mass of the universe has become one of the big challenges of todays particle physics and cosmology. Astronomical observations show that only 1% of the matter of the Universe is luminous. Moreover there is now convincing evidence that 85% of all gravitationally observable matter in the Universe is of a new exotic kind, different from the 'ordinary' matter surrounding us. In a series of three lectures we discuss past, recent and future efforts made world- wide to detect and/or decipher the nature of Dark Matter. In Lecture I we review our present knowledge of the Dark Matter content of the Universe and how experimenters search for it's candidates; In Lecture II we discuss so-called 'direct detection' techniques which allow to search for scattering of galactic dark matter particles with detectors in deep-underground laboratories; we discuss the interpretation of experimental results and the challenges posed by different backgrounds; In Lecture III we take a look at the 'indirect detection' of the annihilation of dark matter candidates in astrophysical objects, such as our sun or the center of the Milky Way; In addition we will have a look at efforts to produce Dark Matter particles directly at accelerators and we shall close with a look at alternative nonparticle searches and future prospects. (author)

  18. Complexes of 4-chlorophenoxyacetates of Nd(III), Gd(III) and Ho(III)

    International Nuclear Information System (INIS)

    Ferenc, W.; Bernat, M; Gluchowska, H.W.; Sarzynski, J.

    2010-01-01

    The complexes of 4-chlorophenoxyacetates of Nd(III), Gd(III) and Ho(III) have been synthesized as polycrystalline hydrated solids, and characterized by elemental analysis, spectroscopy, magnetic studies and also by X-ray diffraction and thermogravimetric measurements. The analysed complexes have the following colours: violet for Nd(III), white for Gd(III) and cream for Ho(III) compounds. The carboxylate groups bind as bidentate chelating (Ho) or bridging ligands (Nd, Gd). On heating to 1173K in air the complexes decompose in several steps. At first, they dehydrate in one step to form anhydrous salts, that next decompose to the oxides of respective metals. The gaseous products of their thermal decomposition in nitrogen were also determined and the magnetic susceptibilities were measured over the temperature range of 76-303K and the magnetic moments were calculated. The results show that 4-chlorophenoxyacetates of Nd(III), Gd(III) and Ho(III) are high-spin complexes with weak ligand fields. The solubility value in water at 293K for analysed 4-chlorophenoxyacetates is in the order of 10 -4 mol/dm 3 . (author)

  19. DNA polymerase iota (Pol ι) promotes invasion and metastasis of esophageal squamous cell carcinoma.

    Science.gov (United States)

    Zou, Shitao; Shang, Zeng-Fu; Liu, Biao; Zhang, Shuyu; Wu, Jinchang; Huang, Min; Ding, Wei-Qun; Zhou, Jundong

    2016-05-31

    DNA polymerase iota (Pol ι) is an error-prone DNA polymerase involved in translesion DNA synthesis (TLS) that contributes to the accumulation of DNA mutations. We recently showed that Pol ι is overexpressed in human esophageal squamous cell cancer (ESCC) tissues which promotes ESCC' progression. The present study was aimed at investigating the molecular mechanisms by which Pol ι enhances the invasiveness and metastasis of ESCC cells. We found that the expression of Pol ι is significantly higher in ESCCs with lymph node metastasis compared to those without lymph node metastasis. Kaplan-Meier analysis revealed an inverse correlation between Pol ι expression and patient prognosis. The expression levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), two essential regulators of cells' invasiveness, were positively associated with Pol ι expression in ESCC tissues. Ectopic expression of Pol ι enhanced the motility and invasiveness of ESCC cells as evaluated by wound-healing and transwell assays, respectively. A xenograft nude mouse model showed that Pol ι promotes the colonization of ESCC cells in the liver, lung and kidney. Signaling pathway analysis identified the JNK-AP-1 cascade as a mediator of the Pol ι-induced increase in the expression of MMP-2/9 and enhancement of ESCC progression. These data demonstrate the underlying mechanism by which Pol ι promotes ESCC progression, suggesting that Pol ι is a potential novel prognostic biomarker and therapeutic target for ESCC.

  20. O trabalho familiar extrativista sob a influência de políticas públicas

    Directory of Open Access Journals (Sweden)

    Dalva Maria da Mota

    2014-01-01

    Full Text Available O artigo analisa a relação entre a organização do trabalho familiar no extrativismo e a participação em programas de políticas públicas no estado de Sergipe. Com abordagem predominantemente qualitativa, a pesquisa foi realizada com mulheres autodesignadas catadoras de mangaba e marisqueiras, reconhecidas como pertinentes ao segmento dos denominados povos e comunidades tradicionais e afiliadas ao Programa Bolsa Família (PBF, ao Seguro Desemprego do Pescador Artesanal (SDPA e ao Programa de Aquisição de Alimentos (PAA. A metodologia constou de observações e entrevistas com diferentes atores envolvidos na atividade extrativista e nos programas de políticas públicas. As principais conclusões mostram que os programas de políticas públicas influenciam: i na reorganização do cotidiano do trabalho no extrativismo, principalmente no tocante à diminuição do envolvimento de crianças e jovens na atividade e quanto à intensidade das jornadas; ii no reforço aos papéis tradicionais de homens e mulheres, no caso do PBF, e na diluição de fronteiras entre esses mesmos papéis no PAA; iii na diminuição do volume de trabalho no caso do SDPA e no aumento no PAA; e iv nos diferentes sentidos que são atribuídos ao trabalho.

  1. A reestruturação político-administrativa do Império Romano na Época de Diocleciano e da Tetrarquia (284-305

    Directory of Open Access Journals (Sweden)

    Diogo Pereira Silva

    2017-10-01

    Full Text Available Neste artigo, temos por objetivo analisar os fundamentos da reestruturação político-administrativa do Império Romano durante o governo de Diocleciano, considerando as pré-condições do século III, e as características que conformaram o poder imperial romano durante a época de Diocleciano e da Tetrarquia (284-305.

  2. Repriming by PrimPol is critical for DNA replication restart downstream of lesions and chain-terminating nucleosides.

    Science.gov (United States)

    Kobayashi, Kaori; Guilliam, Thomas A; Tsuda, Masataka; Yamamoto, Junpei; Bailey, Laura J; Iwai, Shigenori; Takeda, Shunichi; Doherty, Aidan J; Hirota, Kouji

    2016-08-02

    PrimPol is a DNA damage tolerance enzyme possessing both translesion synthesis (TLS) and primase activities. To uncover its potential role in TLS-mediated IgVλ hypermutation and define its interplay with other TLS polymerases, PrimPol(-/-) and PrimPol(-/-)/Polη(-/-)/Polζ (-/-) gene knockouts were generated in avian cells. Loss of PrimPol had no significant impact on the rate of hypermutation or the mutation spectrum of IgVλ. However, PrimPol(-/-) cells were sensitive to methylmethane sulfonate, suggesting that it may bypass abasic sites at the IgVλ segment by repriming DNA synthesis downstream of these sites. PrimPol(-/-) cells were also sensitive to cisplatin and hydroxyurea, indicating that it assists in maintaining / restarting replication at a variety of lesions. To accurately measure the relative contribution of the TLS and primase activities, we examined DNA damage sensitivity in PrimPol(-/-) cells complemented with polymerase or primase-deficient PrimPol. Polymerase-defective, but not primase-deficient, PrimPol suppresses the hypersensitivity of PrimPol(-/-) cells. This indicates that its primase, rather than TLS activity, is pivotal for DNA damage tolerance. Loss of TLS polymerases, Polη and Polζ has an additive effect on the sensitivity of PrimPol(-/-) cells. Moreover, we found that PrimPol and Polη-Polζ redundantly prevented cell death and facilitated unperturbed cell cycle progression. PrimPol(-/-) cells also exhibited increased sensitivity to a wide variety of chain-terminating nucleoside analogs (CTNAs). PrimPol could perform close-coupled repriming downstream of CTNAs and oxidative damage in vitro. Together, these results indicate that PrimPol's repriming activity plays a central role in reinitiating replication downstream from CTNAs and other specific DNA lesions.

  3. PREFACE: Quantum Optics III

    Science.gov (United States)

    Orszag, M.; Retamal, J. C.; Saavedra, C.; Wallentowitz, S.

    2007-06-01

    All the 50 years of conscious pondering did not bring me nearer to an answer to the question `what is light quanta?'. Nowadays, every rascal believes, he knows it, however, he is mistaken. (A Einstein, 1951 in a letter to M Besso) Quantum optics has played a key role in physics in the last several decades. On the other hand, in these early decades of the information age, the flow of information is becoming more and more central to our daily life. Thus, the related fields of quantum information theory as well as Bose-Einstein condensation have acquired tremendous importance in the last couple of decades. In Quantum Optics III, a fusion of these fields appears in a natural way. Quantum Optics III was held in Pucón, Chile, in 27-30 of November, 2006. This beautiful location in the south of Chile is near the lake Villarrica and below the snow covered volcano of the same name. This fantastic environment contributed to a relaxed atmosphere, suitable for informal discussion and for the students to have a chance to meet the key figures in the field. The previous Quantum Optics conferences took place in Santiago, Chile (Quantum Optics I, 2000) and Cozumel, Mexico (Quantum Optics II, 2004). About 115 participants from 19 countries attended and participated in the meeting to discuss a wide variety of topics such as quantum-information processing, experiments related to non-linear optics and squeezing, various aspects of entanglement including its sudden death, correlated twin-photon experiments, light storage, decoherence-free subspaces, Bose-Einstein condensation, discrete Wigner functions and many more. There was a strong Latin-American participation from Argentina, Brazil, Chile, Colombia, Peru, Uruguay, Venezuela and Mexico, as well as from Europe, USA, China, and Australia. New experimental and theoretical results were presented at the conference. In Latin-America a quiet revolution has taken place in the last twenty years. Several groups working in quantum optics and

  4. Políticas de seguridad informática

    Directory of Open Access Journals (Sweden)

    Ciro Antonio Dussan Clavijo

    2006-01-01

    Full Text Available La globalización de la economía ha exigido que las empresas implementen plataformas tecnológicas que soporten la nueva forma de hacer negocios. El uso de Internet para este fin, conlleva a que se desarrollen proyectos de seguridad informática que garanticen la integridad, disponibilidad y accesibilidad de la información. La creación de políticas de seguridad es una labor fundamental que involucra las personas, los procesos y los recursos de la compañía. Este articulo presenta los puntos clave a tener en cuenta para diseñar una política de seguridad basándose en la norma ISO 17799.

  5. Os direitos humanos e a política internacional

    Directory of Open Access Journals (Sweden)

    Rossana Rocha Reis

    2006-11-01

    Full Text Available O artigo trata da questão do crescente reconhecimento internacional dos direitos humanos desde o fim da II Guerra Mundial e discute os efeitos desse processo sobre a política internacional. De modo geral os argumentos sobre o papel dos direitos humanos na política internacional dividem-se entre os que acham que eles não passam de retórica para encobrir interesses particulares, e os que enxergam na sua afirmação um potencial transformador da ordem internacional. No contexto atual,em que se discute a adoção de mecanismos coercitivos mais fortes para a proteção dos direitos humanos, como as intervenções humanitárias, por exemplo, essa discussão torna-se mais complexa e mais urgente.

  6. A política da guerra em Maquiavel

    OpenAIRE

    Coelho,Maria Francisca Pinheiro; Menezes,Marilde Loiola de

    2013-01-01

    Ao considerar que os objetivos do governante são a conquista e a manutenção do poder, Nicolau Maquiavel sugere ser o estado de guerra uma situação permanente na arte de governar. Este artigo aborda o pensamento político do autor, a partir de sua análise sobre a guerra. Visa apreender o sentido que ele confere à política e os fundamentos de uma teoria sobre a guerra, construída com base no diálogo com as experiências dos antigos e as circunstâncias históricas de seu tempo. O trabalho está estr...

  7. Gênero e política no pensamento de Maquiavel

    OpenAIRE

    Pitkin, Hannah

    2013-01-01

    O texto é parte de uma análise mais ampla da teórica política Hanna Pitkin sobre as relações de gênero na obra de Nicolau Maquiavel. Apresenta uma análise das representações do feminino nos textos do autor florentino. Há um inventário detalhado, no artigo, de quais são as imagens das mulheres e como elas são mobilizadas. Com ele, Pitkin discute as implicações políticas dessas imagens. A oposição entre o masculino e o feminino é apresentada como um componente importante das compreensões do pod...

  8. Contextos políticos pre-electorales

    OpenAIRE

    Iñíguez Rímoli, Nathalie; Fernández, Guillermo

    2007-01-01

    El presente artículo está enmarcado en el proyecto de investigación “El éxito de las campañas políticas locales en el contexto de campañas de carácter nacional en la Argentina: el caso de la ciudad de La Plata en las elecciones de los años 1999 y 2005”. En el mismo se busca establecer la relación correspondiente entre las campañas electorales de los años 1999 y 2005, para lograr la caracterización del contexto de construcción y legitimación de las campañas políticas locales en el marco de las...

  9. Hopf Bifurcation of Compound Stochastic van der Pol System

    Directory of Open Access Journals (Sweden)

    Shaojuan Ma

    2016-01-01

    Full Text Available Hopf bifurcation analysis for compound stochastic van der Pol system with a bound random parameter and Gaussian white noise is investigated in this paper. By the Karhunen-Loeve (K-L expansion and the orthogonal polynomial approximation, the equivalent deterministic van der Pol system can be deduced. Based on the bifurcation theory of nonlinear deterministic system, the critical value of bifurcation parameter is obtained and the influence of random strength δ and noise intensity σ on stochastic Hopf bifurcation in compound stochastic system is discussed. At last we found that increased δ can relocate the critical value of bifurcation parameter forward while increased σ makes it backward and the influence of δ is more sensitive than σ. The results are verified by numerical simulations.

  10. Crystal structure of DNA polymerase III β sliding clamp from Mycobacterium tuberculosis.

    Science.gov (United States)

    Gui, Wen-Jun; Lin, Shi-Qiang; Chen, Yuan-Yuan; Zhang, Xian-En; Bi, Li-Jun; Jiang, Tao

    2011-02-11

    The sliding clamp is a key component of DNA polymerase III (Pol III) required for genome replication. It is known to function with diverse DNA repair proteins and cell cycle-control proteins, making it a potential drug target. To extend our understanding of the structure/function relationship of the sliding clamp, we solved the crystal structure of the sliding clamp from Mycobacterium tuberculosis (M. tuberculosis), a human pathogen that causes most cases of tuberculosis (TB). The sliding clamp from M. tuberculosis forms a ring-shaped head-to-tail dimer with three domains per subunit. Each domain contains two α helices in the inner ring that lie against two β sheets in the outer ring. Previous studies have indicated that many Escherichia coli clamp-binding proteins have a conserved LF sequence, which is critical for binding to the hydrophobic region of the sliding clamp. Here, we analyzed the binding affinities of the M. tuberculosis sliding clamp and peptides derived from the α and δ subunits of Pol III, which indicated that the LF motif also plays an important role in the binding of the α and δ subunits to the sliding clamp of M. tuberculosis. Copyright © 2011 Elsevier Inc. All rights reserved.

  11. Governamentalidade e a genealogia da política

    Directory of Open Access Journals (Sweden)

    Colin Gordon

    2013-12-01

    Full Text Available O artigo oferece uma série de reflexões a respeito de uma fase dos trabalhos de Michel Foucault e de alguns de seus colaboradores apresentada no volume The Foucault effect: studies in governmentality (1991, coeditado pelo autor. Tais reflexões se organizam em três partes. Primeiro, há uma revisão de alguns aspectos das aulas de Foucault sobre governamentalidade que, por diferentes razões, foram deixados de lado quando o livro foi publicado. Em seguida, faz-se um balanço de temas importantes que, embora presentes no livro, não receberam suficiente atenção dos leitores. Finalmente, no eixo que ocupa a maior parte do artigo, examinam-se as últimas discussões de Foucault a respeito do que o autor denomina múltiplos nascimentos da política, a fim de demonstrar a continuidade da pertinência do empreendimento foucaultiano nos anos 1970, tornado possível devido à noção de governamentalidade, ao mesmo tempo tão estranha e operacional. Tal atualidade é indicada não somente pelo incremento dos estudos sobre governamentalidade após o aparecimento dos cursos que Foucault deu no Collège de France, mas principalmente pelos dilemas e aporias que a cultura política em que estamos mergulhados nos trouxe. Nesse sentido, o artigo se encerra com uma espécie de agenda de pesquisa para dar continuidade ao trabalho inacabado de Foucault, uma agenda que nos convida a aprofundar nossa compreensão das relações entre a filosofia crítica, a racionalidade política e a cultura política, compreendidas como um conjunto de formas de conduta e de sociabilidade, modos de vida e estilos de subjetivação e dizer verdadeiro.

  12. Política oriental de India: Mitos y realidades

    Directory of Open Access Journals (Sweden)

    Vaidyanathan Shivkumar

    2015-07-01

    Full Text Available La política exterior de la India desde su independencia el 15 agosto de 1947, ha sido de cooperación y conciliación, y no de confrontación. Ésta podría haber sido ampliada cuando los infiltrados pakistaníes entraron en el territorio de la India en 1948, ya que el entonces Primer Ministro instó a que este asunto se remitiera al recién formado Consejo Mundial de Naciones Unidas. Si se recorre la historia, encontraríamos que las tres guerras entre India y Pakistán fueron iniciadas por Pakistán ante lo cual la India tuvo que defender su territorio. Similar situación surgió cuando China rompió unilateralmente el acuerdo de Pancha Sheel, invadiendo el territorio de la India. Este artículo hace un modesto intento de trazar la política de la India hacia el cercano oriente o hacia las naciones del sureste. Describe los vínculos históricos y culturales entre la India y las naciones del sur de Asia oriental y la consolidación definitiva de la “política hacia el oriente de la India”. En este artículo se toman en consideración los intereses económicos y estratégicos de la región y se espera que pudiera permitir a los lectores entender la política de la India hacia las naciones del sudeste de Asia.

  13. Áreas de polígonos via determinantes

    OpenAIRE

    Zerbinatti, Paulo Henrique [UNESP

    2015-01-01

    The aim of this work is to present a study on areas of polygons through their vertex coordinates. We treat the subject using determinants of order 2 and basic Euclidean Geometry O objetivo deste trabalho e apresentar um estudo sobre o c alculo de areas de pol gonos atrav es das coordenadas de seus v ertices. Faremos isto utilizando determinantes de ordem 2 e conceitos b asicos de Geometria Euclidiana Plana

  14. FHC: o intelectual como político

    Directory of Open Access Journals (Sweden)

    Celso Lafer

    2009-03-01

    Full Text Available Este artigo parte do exame dos múltiplos papéis que nas sociedades contemporâneas os intelectuais desempenham na vida política. Aponta a tendência à imperícia no trato da realidade política por parte dos intelectuais no exercício do poder. Neste contexto, discute-se como e por que FHC é um raro caso de um intelectual bem-sucedido na vida política de um país complexo, de escala continental, com as características do Brasil contemporâneo. Aquilata-se, finalmente, a maneira pela qual FHC conjugou teoria e experiência nos seus juízos políticos perante as especificidades das conjunturas com as quais lidou na presidência e como combinou, na sua liderança, a dimensão da mudança e da pacificação, tendo sempre presente o "quadro mental" e o sentido de direção da sua visão intelectual.The point of departure of the article is a discussion of the social roles intellectuals play in the political life of modern societies. Subsequently an analysis of the difficulties that intellectuals tend to have, when exercising power, in dealing with political realities is presented. In this context the article discusses the why and the how FHC is a rare case of a successful intellectual in the politics of a complex democratic country, of continental dimension, with the specificities of contemporary Brazil. The association of theory and experience in the political judgment of FHC in dealing with the realities he faced as President is explored as well as his style of leadership that blended change and pacification, always bearing in mind the sense of direction of his outlook as an intellectual.

  15. Políticas del posgrado en México

    Directory of Open Access Journals (Sweden)

    Víctor Martiniano Arredondo Galván

    2006-01-01

    Full Text Available El artículo define, documenta y analiza la evolución de las políticas públicas de la educación superior en México, específicamente del posgrado. Se subraya que el desarrollo del posgrado en el país ha estado supeditado al devenir de la educación superior y al de la ciencia y la tecnología. A su vez se hace hincapié en que las políticas públicas han sido promovidas e instrumentadas por una diversidad de organismos e instancias ubicadas en la administración pública. Un punto medular del trabajo está en las consideraciones en torno a las políticas públicas del posgrado actual; las cuales están supeditadas y gravitan sobre el instrumento que conjuntamente la SEP y el CONACYT han establecido en los últimos años: el Programa de Fortalecimiento del Posgrado Nacional, el cual instituye un nuevo Padrón Nacional de Posgrado que clasifica los programas como competentes a nivel internacional y como de alto nivel; así como su reconocimiento en cuanto a su complejidad y heterogeneidad.

  16. Controlling optics contamination at the PolLux STXM

    Science.gov (United States)

    Watts, B.; Pilet, N.; Sarafimov, B.; Witte, K.; Raabe, J.

    2018-04-01

    Contamination of X-ray mirror surfaces by carbon is a common issue that can significantly degrade the optical performance of the instrument. The effects can be severe at photon energies near the carbon K-edge (ca. 300 eV), where the X-rays are strongly attenuated, but also significant at higher photon energies where the carbon coating affects the reflectivity and surface shape of the mirrors. [1] The Swiss Light Source has typically relied on in-situ plasma cleaning to control mirror contamination and the PolLux scanning transmission X-ray microscopy (STXM) beamline has also been employing further contamination reduction strategies in recent years. In particular, in 2014 we installed a 1×10‑8 mbar background pressure of O2 on the PolLux first mirror chamber. We present a history of efforts to control optical contamination at the PolLux beamline and report on the observed efficiencies of the different processes employed both for the in-vacuum optics and critical components of the frequently vented STXM experiment chamber.

  17. Política de antibióticos

    Directory of Open Access Journals (Sweden)

    Juan José Pisonero Socías

    Full Text Available La utilización inadecuada de los antimicrobianos ha traído como consecuencia un incremento de la resistencia de los microorganismos y un aumento en los costos hospitalarios. Esta situación provoca mala calidad en el servicio. Durante 2 años se aplicó una política racional inducida a reducir el uso, o poner en reposo, a los antimicrobianos poco efectivos o inefectivos, en todo el servicio médico del Hospital Clinicoquirúrgico Docente "Joaquín Albarrán", de Ciudad de La Habana, Cuba. Se aplicaron procederes y métodos creados por un equipo multidisciplinario. La forma de restricción del uso de antimicrobianos, a partir de un Grupo de Control, muestra alentadores resultados. En el trabajo se explican las funciones de este grupo. Se obtuvieron resultados concretos en la disminución de la resistencia microbiana frente a los antimicrobianos más utilizados. Son significativos los datos que muestran la reducción de costos y volúmenes de antibióticos usados en relación con el año de referencia, período en el que aún no se practicaba la política actual. La investigación demostró, que esta política es aplicable en cualquier hospital, a bajo costo y con resultados a corto plazo

  18. Bartleby o la política del caos

    Directory of Open Access Journals (Sweden)

    Andrés Camilo Torres Estrada

    2013-01-01

    Full Text Available El artículo presenta un análisis del cuento de Herman Melville: “Bartleby, el escribiente”. Este análisis parte de un acercamiento a la literatura desde una noción de política inspirada en autores como Jacques Derrida y Jacques Rancière, pero que se aparta de esta filosofía cuando, por encima del propio texto y sus posibilidades, se pretende encontrar en él heterotopías, esperanzas y comunidades por venir. Esta noción de política acerca a la literatura al mundo efectivo, transfigurando los modos de ser y no ser. Así, el personaje de Bartleby se presenta en medio del mundo de la representación como un agente del caos. En esto consiste la política del escribiente: su incursión devela las farsas del mundo construido y soñado por los hombres; pero esta destrucción que causa es inútil: el mundo de la representación ha sentido su absurdo, no sabe qué hacer con dicho agente del caos, pero no es suficiente para arruinarlo. Bartleby, quien se ha indeterminado y ha abandonado las dualidades de ser y no ser, tampoco puede seguir viviendo: ya no hay espacio para la vida sin referencias en un mundo humano y ficticio.

  19. Reformas constitucionales y competencia política en Bolivia

    Directory of Open Access Journals (Sweden)

    Mario Torrico

    2017-03-01

    El presente artículo explica  las  causas  y  efectos del cambio institucional en contextos políticos en los que no hay garantía sobre el cumplimiento estricto de  las reglas.  Analizando  la  reforma  política  de  2005  que  posibilitó  (por primera vez  que   en   Bolivia   los   prefectos   o  gobernadores  puedan  ser elegidos por voto popular,  se  halla  evidencia  que  indica  que  cuando  hay crisis  de  legitimidad,  las  coaliciones  declinantes  impulsan  cambios inclu-yentes  que  reducen   sus   futuras   pérdidas  electorales  y  no  pueden  ser revertidos por las coaliciones ascendentes que llegan al poder. No obstante, éstas intentan limitar el grado de inclusión de las reglas a través de cambios normativos de menor nivel que disminuyen la competencia política.

  20. La política supranacional : un nuevo campo de conocimiento para abordar las políticas educativas en un mundo globalizado

    OpenAIRE

    Valle, Javier M.

    2012-01-01

    El presente artículo trata de explicar la aparición de la Política Educativa Supranacional como área del saber pedagógico, en íntima conexión con la Política Educativa, la Educación Comparada y la Educación Internacional, tratando de acotar s

  1. Política nacional de assistência social: uma avaliação política

    OpenAIRE

    Araujo, Victor Martins Lopes de

    2013-01-01

    Este trabalho tem com objetivo principal realizar uma avaliação política da Política Nacional de Assistência Social através de uma leitura crítica de seus textos legais. Nesse sentido, realizou-se uma fundamentação teórica pautada na discussão das diferentes concepções de Estado encontradas na teoria social clássica bem como na teoria social crítica, sendo feito também uma discussão a respeito da administração como forma de dominação, nos apropriamos do debate acerca da burocracia presente em...

  2. Basel III D: Swiss Finish to Basel III

    OpenAIRE

    Christian M. McNamara; Natalia Tente; Andrew Metrick

    2014-01-01

    After the Basel Committee on Banking Supervision (BCBS) introduced the Basel III framework in 2010, individual countries confronted the question of how best to implement the framework given their unique circumstances. Switzerland, with a banking industry that is both heavily concentrated and very large relative to the size of its overall economy, faced a special challenge. It ultimately adopted what is sometimes referred to as the “Swiss Finish” to Basel III – enhanced requirements applicable...

  3. Outcome of tyrosinaemia type III.

    Science.gov (United States)

    Ellaway, C J; Holme, E; Standing, S; Preece, M A; Green, A; Ploechl, E; Ugarte, M; Trefz, F K; Leonard, J V

    2001-12-01

    Tyrosinaemia type III is a rare disorder caused by a deficiency of 4-hydroxyphenylpyruvate dioxygenase, the second enzyme in the catabolic pathway of tyrosine. The majority of the nine previously reported patients have presented with neurological symptoms after the neonatal period, while others detected by neonatal screening have been asymptomatic. All have had normal liver and renal function and none has skin or eye abnormalities. A further four patients with tyrosinaemia type III are described. It is not clear whether a strict low tyrosine diet alters the natural history of tyrosinaemia type III, although there remains a suspicion that treatment may be important, at least in infancy.

  4. Thermodecomposition of lanthanides (III) and ytrium (III) glucoheptonates

    International Nuclear Information System (INIS)

    Giolito, J.

    1987-01-01

    The lanthanides (III) and yttrium (III) glucoheptonates as well the D-glucoheptono 1-4 lactone were studied using common analytical methods, elemental microanalysis of carbon and hydrogen, thermogravimetry and differential scanning calorimetry. These compounds were prepared from the reaction between the lanthanides (III) and yttrium (III) hydroxides and glucoheptonic acid aqueous solution obtained by means of the delta lactone hydrolysis of this acid. After stoichiometric reaction the compounds were precipitated by the addition of absolute ethanol, washed with the same solvent and dried in desiccator. Thermogravimetric the (TG) curves of the lanthanides glucoheptonates of the ceric group present thermal profiles with enough differences permitting an easy caracterization of each compound and the yttrium (III) glucoheptonate TG curve showed a great similarity with the erbium (III) compound TG curve. The differential scanning calometry (DSC) curves showed endothermic and exothermic peaks by their shape, height and position (temperature) permit an easy and rapid identification of each compound specially if DSC and TG curves were examined simultaneously. (author) [pt

  5. Seminario Políticas Públicas y Gestión Estatal | Tema 2: Ciclo y agenda de las políticas públicas

    OpenAIRE

    Pagani, María Laura

    2017-01-01

    Presentación general del ciclo de política pública y las diferentes etapas. Sus limitaciones como recurso de análisis de las PP. Agenda Social y Agenda Política: los procesos de construcción de la Agenda política, diseño. Definición de política pública. Presentación general del ciclo de política pública y las diferentes etapas. Sus limitaciones como recurso de análisis de las PP. La formación de la agenda y la definición de problema público. Las diferentes agendas: la agenda ciudadana, públic...

  6. An isolated Hda-clamp complex is functional in the regulatory inactivation of DnaA and DNA replication.

    Science.gov (United States)

    Kawakami, Hironori; Su'etsugu, Masayuki; Katayama, Tsutomu

    2006-10-01

    In Escherichia coli, a complex consisting of Hda and the DNA-loaded clamp-subunit of the DNA polymerase III holoenzyme promotes hydrolysis of DnaA-ATP. The resultant ADP-DnaA is inactive for initiation of chromosomal DNA replication, thereby repressing excessive initiations. As the cellular content of the clamp is 10-100 times higher than that of Hda, most Hda molecules might be complexed with the clamp in vivo. Although Hda predominantly forms irregular aggregates when overexpressed, in the present study we found that co-overexpression of the clamp with Hda enhances Hda solubility dramatically and we efficiently isolated the Hda-clamp complex. A single molecule of the complex appears to consist of two Hda molecules and a single clamp. The complex is competent in DnaA-ATP hydrolysis and DNA replication in the presence of DNA and the clamp deficient subassembly of the DNA polymerase III holoenzyme (pol III*). These findings indicate that the clamp contained in the complex is loaded onto DNA through an interaction with the pol III* and that the Hda activity is preserved in these processes. The complex consisting of Hda and the DNA-unloaded clamp may play a specific role in a process proceeding to the DnaA-ATP hydrolysis in vivo.

  7. Transformational III-V Electronics

    KAUST Repository

    Nour, Maha A.

    2014-01-01

    Flexible electronics using III-V materials for nano-electronics with high electron mobility and optoelectronics with direct band gap are attractive for many applications. This thesis describes a complementary metal oxide semiconductor (CMOS

  8. Complexes of lanthanum(III), cerium(III), samarium(III) and dysprosium(III) with substituted piperidines

    Energy Technology Data Exchange (ETDEWEB)

    Manhas, B S; Trikha, A K; Singh, H; Chander, M

    1983-11-01

    Complexes of the general formulae M/sub 2/Cl/sub 6/(L)/sub 3/.C/sub 2/H/sub 5/OH and M/sub 2/(NO/sub 3/)/sub 6/(L)/sub 2/.CH/sub 3/OH have been synthesised by the reactions of chlorides and nitrates of La(III), Ce(III), Sm(III) and Dy(III) with 2-methylpiperidine, 3-methylpiperidine and 4-methylpiperidine. These complexes have been characterised on the basis of their elemental analysis, and IR and electronic reflectance spectra. IR spectral data indicate the presence of coordinated ethanol and methanol molecules and bidentate nitrate groups. Coordination numbers of the metal ions vary from 5 to 8. 19 refs.

  9. Celestine III and the North

    DEFF Research Database (Denmark)

    Nielsen, Torben Kjersgaard

    2008-01-01

    Artiklen gennemgår pave Cølestin IIIs forhold til de nordiske kongeriger i perioden 1191-1198. Artiklen viser, at paven, som i forskningen traditionelt år har stået i skyggen af sin berømte, energiske og især: yngre efterfølger, Innocens III, har været på forkant med udviklingen i de nordiske rig...

  10. Los efectos de la comunicación política en el comportamiento electoral

    Directory of Open Access Journals (Sweden)

    Ismael Crespo Martínez

    2015-07-01

    Full Text Available La comunicación política es un aspecto fundamental de todos los procesos políticos contemporáneos. De hecho, el análisis político de la realidad, bien sea para realizar cálculos estratégicos, o bien para tratar de profundizar en la comprensión de las dinámicas socio-políticas, ya no puede obviar el papel central de la comunicación política en las sociedades. A partir de esta afirmación, los autores exponen a través de un recorrido histórico la influencia y efectos de la comunicación en la política, incluyendo las nuevas tendencias con los avances de las tecnologías de la comunicación

  11. Espacios de socialización política y representaciones de la política en la Universidad de Antioquia

    Directory of Open Access Journals (Sweden)

    Deicy Patricia Hurtado Galeano

    2017-01-01

    Full Text Available Este artículo describe los procesos de socialización política de los universitarios a través de las interacciones cara a cara —en la familia, en el vecindario, en la universidad— y de los medios de comunicación análogos —televisión, radio, prensa— y virtuales —redes sociales, blogs—. Una vez analizado cómo se informan sobre política y en qué espacios hablan de estos temas, se analiza el interés en la política, así como las imágenes que los universitarios tienen de esta. Con base en estas variables se concluye que la Universidad es un entorno político en el que no puede verificase una apatía política en los actores clave de la vida universitaria —estudiantes, profesores y empleados administrativos—, no solo porque sí les interesan estos asuntos, sino porque se informan y conversan sobre política en distintos espacios de socialización, se sienten con capacidades y competencias para enfrentar esa esfera, y predomina una visión crítica de la política y de la forma como es conducida en la sociedad colombiana.

  12. Seguridad humana y recursos naturales - política de seguridad en Colombia

    OpenAIRE

    Echeverría Rodríguez, Aura María

    2010-01-01

    El presente trabajo de grado atiende el nuevo concepto de política (biopolítica) que ha sido definido, por algunos autores como la política alrededor de la vida (bios), y el nuevo concepto de seguridad (seguridad humana) que cobija dentro de sus dimensiones, la seguridad medio ambiental. Esos nuevos conceptos justifican el interés de hacer un análisis de la política de seguridad en Colombia, especialmente, de la política de seguridad frente a los recursos naturales que son el fundamento de la...

  13. Políticas sobre Sexualidad: Reportes desde las Líneas del Frente

    OpenAIRE

    Petchesky, Rosalind; Vianna, Adriana R. B.; Carrara, Sergio

    2008-01-01

    Políticas sobre sexualidad: reportes desde las líneas del frente, es un estudio comparativo de las políticas de sexualidad, la salud sexual y los derechos sexuales en ochos países y en dos instituciones mundiales. El trabajo contenido en Políticas sobre sexualidad ha sido desarrollado bajo el auspicio del Sexual Policy Watch [Observatorio de Políticas Sexuales] (SPW), un foro mundial compuesto por investigadores y activistas de una amplia gama de países y regiones del mundo. Inspirado en inic...

  14. Chaos excited chaos synchronizations of integral and fractional order generalized van der Pol systems

    International Nuclear Information System (INIS)

    Ge Zhengming; Hsu Maoyuan

    2008-01-01

    In this paper, chaos excited chaos synchronizations of generalized van der Pol systems with integral and fractional order are studied. Synchronizations of two identified autonomous generalized van der Pol chaotic systems are obtained by replacing their corresponding exciting terms by the same function of chaotic states of a third nonautonomous or autonomous generalized van der Pol system. Numerical simulations, such as phase portraits, Poincare maps and state error plots are given. It is found that chaos excited chaos synchronizations exist for the fractional order systems with the total fractional order both less than and more than the number of the states of the integer order generalized van der Pol system

  15. As políticas activas e passivas do mercado de trabalho

    OpenAIRE

    Novo, Álvaro; Centeno, Mário

    2008-01-01

    A generalidade dos países utiliza políticas destinadas a minorar os custos sociais e individuais do desemprego. Os economistas classificam estas políticas do mercado de trabalho em dois grupos: (i) políticas activas e (ii) políticas passivas. As primeiras têm como objectivo dotar os desempregados com as qualificações necessárias para minimizar a duração do desemprego, enquanto as segundas visam garantir uma fonte de rendimento durante o período de desemprego, sendo particularmente úteis para ...

  16. Esquema de evaluación para instrumentos de política ambiental

    OpenAIRE

    Mariana Bobadilla; Martha Ileana Espejel Carbajal; Francisco Lara Valencia; Saul Álvarez Borrego; Sophie Ávila Foucat; José Luis Fermán Almada

    2013-01-01

    La evaluación de la política ambiental ha avanzado lentamente en México por varias razones que incluyen la novedad y rápida evolución de los instrumentos de política ambiental; la complejidad de los problemas ambientales y la inexistencia de modelos de evaluación estandarizados para los evaluadores de dicha política. Este artículo propone un esquema metodológico para la evaluación en tres etapas de los instrumentos de política ambiental. El método identifica la funcionalidad del instrumento d...

  17. Nuevas políticas sociales y Estado social relacional.

    Directory of Open Access Journals (Sweden)

    PIERPAOLO DONATI

    2004-01-01

    Full Text Available Según el autor, las sociedades europeas siguen inspirando las políticas sociales en un código (estatal de inclusión ?al que aquí se denomina lib/lab (mezcla de liberalismo y laborismo? que en el pasado ha constituido un factor de progreso, pero que actualmente resulta cada vez más débil y obsoleto. Las políticas sociales encuentran límites estructurales en sus posibilidades de innovación porque están formuladas como compromisos entre Estado y mercado, utilizando al sector terciario para remediar los fracasos de los otros dos sectores. De hecho, los sistemas sociales gobernados por lógicas lib/lab se encaminan a una crisis irreversible. El documento ilustra las nuevas tendencias de configuración de las políticas sociales, que aquí se denominan "societarias ". Se caracterizan por dos novedades principales: primero, por el hecho de que confían la inclusión social a una ciudadanía compleja (concebida como entrelazamiento de ciudadanía estatal y ciudadanía societaria, en la cual poseen un papel primordial los sujetos colectivos de la sociedad civil; segundo, porque definen el Bienestar, los servicios y los derechos sociales mediante un código simbólico de tipo relacional. En términos de orden institucional, se trata de pasar a un "cuarto modelo de Estado del Bienestar", es decir, a un Estado social de cuarta generación, después de haber dejado atrás el paternalista, el asistencial y el intervencionista. El autor lo denomina Estado social relacional.

  18. La creación de partidos políticos en España

    Directory of Open Access Journals (Sweden)

    Juan Francisco Pérez Gálvez

    2011-12-01

    Full Text Available I. Introducción. II. Concepto y diferencia con figuras afines. 1. Concepto. 2. Diferencia con figuras afines. 2.1. Configuración jurídica de la asociación. 2.1.1. Concepto. 2.1.2. Naturaleza jurídica. 2.1.3. Clasificación. A. General. B. Asociaciones de interés general. 2.1.4. Elementos. A. Elementos materiales. B. Elementos formales. 2.1.5. Grupo normativo regulador. 2.1.6. Conclusión. 2.2. Diferencias respecto a la corporación. 2.2.1. Régimen general. 2.2.2. Conclusión. 2.3. Corporación/asociación adversus fundación. 2.3.1. Régimen general. 2.3.2. Conclusión. III. Naturaleza jurídica. IV. Caracteres. V. Grupo normativo regulador. VI. Creación de partidos políticos. 1. Libertad de creación y afiliación. 2. Capacidad para constituir partidos políticos. 3. Constitución y personalidad jurídica. 3.1. Constitución. 3.2. Adquisición de personalidad jurídica. 3.2.1. Aproximación al concepto de persona jurídica. 3.2.2. La tarea propia del jurista: averiguar qué ha de entenderse por persona jurídica cada vez que ese término se emplea en un precepto jurídico. 3.2.3. Régimen jurídico. 4. Inscripción en el registro. 4.1. Concepto y diferencia con figuras afines. 4.2. Naturaleza jurídica. 4.3. Clases. 4.4. Grupo normativo regulador. 4.4.1. Constitución Española. 4.4.2. Ley Orgánica 6/2002, de 27 de junio. 4.4.3. Los registros en la Ley de Régimen Jurídico de las Administraciones Públicas y del Procedimiento Administrativo Común: derecho de acceso.

  19. Democracia liberal e inestabilidad política en Ecuador

    OpenAIRE

    Andrade A., Pablo

    2005-01-01

    Se explora el reciente cambio de gobierno en Ecuador a la luz de las siguientes hipótesis: 1) la inestabilidad política que caracteriza al Ecuador desde hace nueve años; 2) esta fase como resultado de las contradicciones creadas por el intento de reconciliar una economía organizada en torno al libre mercado con los procedimientos de una democracia representativa; 3) el proceso que ha llevado en los últimos trece años a la desarticulación de las instituciones democráticas en Ecuador. Se desarr...

  20. Libertad, equidad y política educativa

    OpenAIRE

    Bonal, Xavier

    2008-01-01

    El autor señala que aquellos que toman las decisiones de política educativa no contemplan las variables necesarias que aseguren la igualdad de oportunidades y la equidad, respecto a la elección de centro, y no atienden a un principio de justicia distributiva que describe en este artículo. Cree que aquel alumnado con un menor nivel social o cultural no está recibiendo, en el modelo actual, la mejor educación posible.

  1. Hacia una historia política de la escritura

    Directory of Open Access Journals (Sweden)

    Rafael Gómez Pardo

    2010-10-01

    Full Text Available En el artículo se presentan algunos de los temas más recurrentes que se pueden abordar en el proyecto de una historia política de la escritura, retomando los planteamientos de Roger Chartier y Michel Foucault. Se define de manera propedéutica el significado y el alcance de este proyecto, y su importancia para los estudios sobre los usos de la escritura en la historia occidental, teniendo en cuenta que en ésta se sedimentan y reproducen poderes, y en su ordenamiento social, emergen nuevos sujetos (el autor, por ejemplo.

  2. Hacia una historia política de la escritura

    OpenAIRE

    Rafael Gómez Pardo

    2010-01-01

    En el artículo se presentan algunos de los temas más recurrentes que se pueden abordar en el proyecto de una historia política de la escritura, retomando los planteamientos de Roger Chartier y Michel Foucault. Se define de manera propedéutica el significado y el alcance de este proyecto, y su importancia para los estudios sobre los usos de la escritura en la historia occidental, teniendo en cuenta que en ésta se sedimentan y reproducen poderes, y en su ordenamiento social, emergen nuevos suje...

  3. Choques de colas anchas y política monetaria

    OpenAIRE

    Ortiz, Marco

    2015-01-01

    Este documento extiende el modelo de Kato y Nishiyama (2005) al introducir choques de colas anchas en un marco neokeynesiano simple, en el que el banco central considera en forma explícita la restricción de no negatividad de la tasa de interés. Los choques con curtosis excesiva hacen que la política monetaria sea más agresiva lejos de la región restringida. Sin embargo, esta diferencia se revierte a medida que la economía se aproxima a la región restringida. Desde un punto de vista cuantitati...

  4. Constant envelope OFDM scheme for 6PolSK-QPSK

    Science.gov (United States)

    Li, Yupeng; Ding, Ding

    2018-03-01

    A constant envelope OFDM scheme with phase modulator (PM-CE-OFDM) for 6PolSK-QPSK modulation was demonstrated. Performance under large fiber launch power is measured to check its advantages in counteracting fiber nonlinear impairments. In our simulation, PM-CE-OFDM, RF-assisted constant envelope OFDM (RF-CE-OFDM) and conventional OFDM (Con-OFDM) are transmitted through 80 km standard single mode fiber (SSMF) single channel and WDM system. Simulation results confirm that PM-CE-OFDM has best performance in resisting fiber nonlinearity. In addition, benefiting from the simple system structure, the complexity and cost of PM-CE-OFDM system could be reduced effectively.

  5. Perú: marketing político y Vladivideos

    Directory of Open Access Journals (Sweden)

    Fernando Palomino V.

    2015-01-01

    Full Text Available En detalle señala las características de las elecciones 2001, convocadas por el Gobierno de transición, tras la caída del régimen de Fujimori. Esboza el perfil de los candidatos que se terciaron; comenta sobre los vladivideos; afirma que fueron los medios de comunicación los protagonistas de las elecciones, definieron la agenda política y fueron determinantes en los resultados finales. La cobertura diaria por televisión de los vladivideos convirtió el tema de la corrupción en show y espectáculo de masas.

  6. Analytical solution for Van der Pol-Duffing oscillators

    International Nuclear Information System (INIS)

    Kimiaeifar, A.; Saidi, A.R.; Bagheri, G.H.; Rahimpour, M.; Domairry, D.G.

    2009-01-01

    In this paper, the problem of single-well, double-well and double-hump Van der Pol-Duffing oscillator is studied. Governing equation is solved analytically using a new kind of analytic technique for nonlinear problems namely the 'Homotopy Analysis Method' (HAM), for the first time. Present solution gives an expression which can be used in wide range of time for all domain of response. Comparisons of the obtained solutions with numerical results show that this method is effective and convenient for solving this problem. This method is a capable tool for solving this kind of nonlinear problems.

  7. La política entre rejas: el devotazo

    Directory of Open Access Journals (Sweden)

    2010-05-01

    Full Text Available En vísperas de la asunción de Héctor Cámpora a la presidencia de la Argentina el 25 de mayo de 1973, el penal de Villa Devoto fue tomado por los prisioneros. Las presiones nacionales e internacionales para la liberación de los detenidos por razones políticas, y el compromiso adoptado por el futuro presidente desde su campaña electoral, lo conducirían a firmar un decreto que sería posteriormente aprobado por el congreso. En ese marco, Alicia Sanguinetti, militante del ERP (Ejército Revoluciona...

  8. Derechos humanos, política y fútbol

    OpenAIRE

    Franco, Marina

    2008-01-01

    La dictadura militar instaurada en 1976 intentó diversas operaciones ideológicas tendientes a la construcción de consenso. Si la más dramática de ellas fue la Guerra de Malvinas, la otra muy significativa -pero por su éxito- fue el Campeonato Mundial de Fútbol de 1978. La realización del evento y la denuncia de una supuesta "campaña antiargentina" desde el exterior fueron dos operaciones mediáticas de la Junta Militar destinadas a construir cierta legitimidad política en el momento mismo en q...

  9. Spectrophotometric and pH-Metric Studies of Ce(III, Dy(III, Gd(III,Yb(III and Pr(III Metal Complexes with Rifampicin

    Directory of Open Access Journals (Sweden)

    A. N. Sonar

    2011-01-01

    Full Text Available The metal-ligand and proton-ligand stability constant of Ce(III, Dy(III, Gd(III,Yb(III and Pr(III metals with substituted heterocyclic drug (Rifampicin were determined at various ionic strength by pH metric titration. NaClO4 was used to maintain ionic strength of solution. The results obtained were extrapolated to the zero ionic strength using an equation with one individual parameter. The thermodynamic stability constant of the complexes were also calculated. The formation of complexes has been studied by Job’s method. The results obtained were of stability constants by pH metric method is confirmed by Job’s method.

  10. Políticas culturales y cultura política en una organización campesina del Magdalena Medio colombiano

    Directory of Open Access Journals (Sweden)

    Nydia Constanza Mendoza Romero

    2011-04-01

    Full Text Available El artículo se orienta a la comprensión de algunas relaciones entre cultura y poder que median los procesos organizativos inscritos en zonas de conflicto social y armado colombiano. En particular, se analiza la forma en la cual, en la configuración histórica de una organización campesina en Cimitarra, se van imbricando las políticas culturales, que desde este tipo de agrupaciones se despliegan, con las culturas políticas locales y nacionales, proceso en el que se van redefiniendo también los modos de asumir lo político.

  11. Observatorio de Política Internacional II. AMERICAS - Ecuador. Política y fútbol

    OpenAIRE

    Neira Fernández, Enrique

    2007-01-01

    Observatorio de Política Internacional II. AMÉRICAS (Neira Fernández, Enrique) RECIENTES Año 2008 Nicaragua. Un remedo revolucionario Bolivia. ¿Una o dislocada? Paraguay. El ex obispo presidente Perú. El rojo sangre de sendero luminoso Estados Unidos. Panorama tras las elecciones primarias RECIENTES Año 2007 Argentina. Contundente triunfo de Cristina Fernández Bolivia. Nuevos rumbos ¡no más embrollo! Ecuador. Nueva constitución, nuevo rumbo Guatemala....

  12. Joint ventures e a política antitruste brasileira

    Directory of Open Access Journals (Sweden)

    Ana Paula Avellar

    2012-12-01

    Full Text Available As políticas de defesa da concorrência integram atualmente o conjunto fundamental de instituições necessárias à promoção do crescimento e a busca das sociedades por arranjos mais eficientes. O presente trabalho tem como objetivo realizar uma análise da política antitruste brasileira concentrando-se no tratamento dado pelas autoridades à constituição de joint ventures. Na realização dessa tarefa, foram feitas revisões teóricas, constatações acerca dos procedimentos de autoridades internacionais sobre o assunto e um estudo empírico abordando casos julgados no Brasil entre os anos de 2002 e 2010. O resultado encontrado revela a inexistência de normatização específica para o tratamento das joint ventures no Brasil, culminando, assim, em uma relativa discricionariedade por parte dos órgãos do SBDC na análise desses atos.

  13. Nuevas pantallas y política audiovisual

    Directory of Open Access Journals (Sweden)

    Francisco Sierra Caballero

    2016-11-01

    Full Text Available La guerra de las pantallas es hoy la quiebra de un orden televisivo en transición a una ecología compleja post Galaxia Marconi, basada en los nuevos hábitos de consumo y de vida. Un problema político, sin ninguna duda, si entendemos que la Comunicación es una Ciencia de lo Común. Una interpretación simple del futuro del audiovisual tiende a poner énfasis solo en las transformaciones tecnológicas. Ciertamente, los cambios en equipamientos, la revolución digital es un factor disruptor del sistema cultural que hay que tomar en cuenta por su relevancia. Ahora bien, insistimos, el acto de ver, la discrecionalidad de la ventana indiscreta nos confronta con el universo ético y político de la mediación como reproducción social. Pues la tecnología no es neutral, ni la comunicación un simple instrumento de transmisión.

  14. Investimentos diretos estrangeiros e políticas de desenvolvimento

    Directory of Open Access Journals (Sweden)

    Antônio Corrêa de Lacerda

    2008-05-01

    Full Text Available O artigo discute alternativas para a inserção internacional da economia brasileira, especialmente no que se refere ao papel dos investimentos diretos estrangeiros, da geração de valor agregado local e do desempenho das exportações, a partir do cenário da globalização e suas transformações. Na primeira parte analisa-se a competitividade dos países em desenvolvimento e, na segunda parte, discutem-se estratégias de política econômica para uma inserção internacional mais ativa na economia brasileira. Palavras-chave: investimentos diretos estrangeiros; globalização; inserção externa; políticas de desenvolvimento. Abstract The article discusses alternatives for the international insertion of Brazilian economy especially considering the role of foreign direct investments, the creation of local aggregated value and the exportations performance from the scene of the globalization and its transformations. In the first part, the competitiveness of developing countries is analyzed and in the second part, strategies of economic policy for a more active international of Brazilian economy are discussed. Keywords: foreign direct investments, globalization, international insertion, development policies.

  15. Política, Democracia y Utopía

    Directory of Open Access Journals (Sweden)

    Mirta A. Giacaglia

    2006-01-01

    Full Text Available La pregunta crucial que hoy nos formulamos es: ¿cómo hacer para reinventar el espacio de lo político en las condiciones instituidas por el proceso de globalización neoliberal? Las últimas décadas se han caracterizado por el eclipse de la política subordinada a la economía y a las fuerzas del mercado. En relación a esta cuestión, clave para pensar la posibilidad de proyectos emancipatorios alternativos, es necesario reflexionar sobre la justicia, la igualdad, la libertad, los lazos sociales, el problema de la representación y los nuevos movimientos sociales de resistencia. Desde esta apelación a los sujetos y a la invención por parte de las fuerzas sociales, es necesario reactivar las fuerzas instituyentes de la utopía para articular libertad e igualdad en el horizonte de otra mundialización. El proyecto de una sociedad justa y solidaria, sólo será posible desde un pensamiento creativo que se atreva a pensar lo no pensado, las líneas de fuga, el acontecimiento.

  16. Cuando lo educativo es político

    Directory of Open Access Journals (Sweden)

    María Victoria Martin

    2016-09-01

    Full Text Available Entre las bases de la Modernidad, encontramos el espacio público como lugar de construcción de ciudadanía y la escuela como dispositivo central para nuclear a los ciudadanos en torno a un proyecto en común. Sin embargo, más de un siglo después de que se instalara la obligatoriedad de la educación en nuestro país mediante la ley 1420, el principio homogeneizador de las instituciones educativas de los Estados nacionales mostró que dejaba por fuera grandes porciones de población. Frente a esto, en la década pasada se gestaron nuevos espacios formativos que buscan priorizar las posibilidades de cambio y que entienden a la educación como instancia política de participación y expresión de los sujetos. Este artículo reflexiona sobre las nociones de espacio público, la relación política-educación y el cambio social articulando estas ideas para concluir con una aproximación a prácticas de comunicación-educación en el partido de Quilmes.

  17. Colombia en torno la prensa política

    Directory of Open Access Journals (Sweden)

    Claudine Bancelin de Emiliani

    2015-05-01

    Full Text Available Para comprender el contenido de los mensajes que lanzan los partidos políticos colombianos -a través de los discursos de sus dirigentes, es necesario esclarecer de dónde provienen dichos mensajes y en qué sociedad concreta se dan, ya que ellos no son un objeto aislado, sino que forman parte de una totalidad, de una estructura, donde los elementos que la conforman están interrelacionados e interactuan entre si. Partiendo del punto de que un ámbito, el quehacer económico es el que determina en última instancia el funcionamiento de una sociedad, el estudio de esta es posible a través del modo de producción dominante. Es así como la estructura económica condiciona la estructura ideológica y la jurídica política. A través del estudio de la formación social es posible comprender la estructura total con su movilidad, donde se llega al momento actual en que la sociedad colombiana está caracterizada por un modo de producción capitalista.

  18. Making progress with PISC III

    International Nuclear Information System (INIS)

    Crutzen, S.; Nichols, R.; McDonald, N.

    1989-01-01

    The thirdphase of the Programme for the Inspection of Steel Components (PISC III) was begun in 1986 with the aim of assessing inspection capability and reliability for actual defects in full scale components under realistic nuclear power plant conditions. It is organized by the OECD Nuclear Energy Agency and the Ispra Joint Research Centre of the European Communities Commission. The objectives and status of each of the seven areas of PISC III are given. The areas are: real contaminated structures; full scale vessel tests; nozzles and dissimilar metal welds; austenitic steel testing; steam generator integrity testing; mathematical modelling of non-destructive examination; and human reliability studies. (U.K.)

  19. Graphics Gems III IBM version

    CERN Document Server

    Kirk, David

    1994-01-01

    This sequel to Graphics Gems (Academic Press, 1990), and Graphics Gems II (Academic Press, 1991) is a practical collection of computer graphics programming tools and techniques. Graphics Gems III contains a larger percentage of gems related to modeling and rendering, particularly lighting and shading. This new edition also covers image processing, numerical and programming techniques, modeling and transformations, 2D and 3D geometry and algorithms,ray tracing and radiosity, rendering, and more clever new tools and tricks for graphics programming. Volume III also includes a

  20. Uma ditadura contra a república: política econômica e poder político em Roberto Campos

    OpenAIRE

    Ricardo V. Silva

    2006-01-01

    O artigo examina o pensamento político de Roberto Campos entre meados das décadas de 1950 e 1970. Neste período, além de importantes funções governamentais, Campos dedicou-se intensamente à luta de idéias, publicando grande quantidade de artigos e ensaios. Será desenvolvida a hipótese de que o seu pensamento político aponta para a institucionalização de um sistema político de tipo autoritário como o mais adequado às condições culturais e políticas da sociedade brasileira. A principal caracter...

  1. Las políticas públicas y la necesidad de una verdadera política social en Venezuela

    Directory of Open Access Journals (Sweden)

    Elys Gilbrando Mora Belandria

    2002-01-01

    Full Text Available El propósito de este artículo consiste en plantear algunos problemas que bloquean el camino hacia el desarrollo político y constituyen tarea urgente para el gobierno. Asimismo se trata de hacer un aporte abordando áreas estratégicas importantes en las estructuras del poder como es el caso de las políticas públicas, tal vez la más reciente e innovadora corriente de análisis presente en la Ciencia Política, incluyendo la política social como una de las exigencias de los ciudadanos frente al Estado y la democracia. Bajo este punto de vista las políticas públicas son consideradas una estrategia básica para legitimar el rendimiento del gobierno. Por último se abordan ciertos resultados de la mala política y sus efectos sobre la democracia pues, así lo entendemos, en Venezuela las tareas del gobierno en materia de políticas públicas han estado marcadas por una visión formalmente bien intencionada, pero que operativamente ha sido bloqueada por factores perturbadores como por ejemplo: la inconsistencia en la toma de decisiones, el centralismo, la corta visión para acercarse a los parámetros de una gerencia pública moderna, la excesiva politización de los trámites administrativos para emprender acciones concretas y la relación asimétrica entre el tamaño del Estado y la calidad del Estado, todo lo cual impide la realización correcta de los procedimientos para profundizar en la tarea obtener logros efectivos y eficientes en materia de política social y el avance hacia la modernización del sector público. .

  2. X-ray polarimetry with the Polarization Spectroscopic Telescope Array (PolSTAR)

    DEFF Research Database (Denmark)

    Krawczynski, Henric S.; Stern, Daniel; Harrison, Fiona A.

    2016-01-01

    This paper describes the Polarization Spectroscopic Telescope Array (PolSTAR), a mission proposed to NASA's 2014 Small Explorer (SMEX) announcement of opportunity. PolSTAR measures the linear polarization of 3-50 keV (requirement; goal: 2.5-70 keV) X-rays probing the behavior of matter,radiation ...

  3. Aproximaciones Sociológicas en Torno a la Cultura Política Colombiana

    OpenAIRE

    Eduardo Guevara Cobos

    1999-01-01

    Partiendo de referentes conceptuales que permiten definir cultura política, se pretende abordar, desde una perspectiva histórica, algunos de sus componentes, con el fin de analizar las actitudes, normas y valores que han caracterizado la cultura política de la sociedad colombiana.

  4. File list: Pol.PSC.10.AllAg.mESCs,_differentiated [Chip-atlas[Archive

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  11. File list: Pol.Utr.10.AllAg.Endometrial_stromal_cells [Chip-atlas[Archive

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  12. File list: Pol.Utr.20.AllAg.Endometrial_stromal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.20.AllAg.Endometrial_stromal_cells hg19 RNA polymerase Uterus Endometrial s...tromal cells SRX1048949 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.20.AllAg.Endometrial_stromal_cells.bed ...

  13. File list: Pol.Neu.05.AllAg.Brain [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  14. File list: Pol.Neu.50.AllAg.Brain [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.50.AllAg.Brain mm9 RNA polymerase Neural Brain SRX236085,ERX132833,SRX01708...2,SRX680480,SRX020249 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.50.AllAg.Brain.bed ...

  15. File list: Pol.Neu.10.AllAg.Brain [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.10.AllAg.Brain mm9 RNA polymerase Neural Brain SRX236085,ERX132833,SRX01708...2,SRX680480,SRX020249 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.10.AllAg.Brain.bed ...

  16. File list: Pol.Neu.20.AllAg.Brain [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.20.AllAg.Brain mm9 RNA polymerase Neural Brain SRX236085,ERX132833,SRX01708...2,SRX680480,SRX020249 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.20.AllAg.Brain.bed ...

  17. File list: Pol.Oth.20.AllAg.Olfactory_epithelium [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  18. File list: Pol.Oth.50.AllAg.Olfactory_epithelium [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.50.AllAg.Olfactory_epithelium mm9 RNA polymerase Others Olfactory epithelium... SRX112963,SRX143827 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.50.AllAg.Olfactory_epithelium.bed ...

  19. File list: Pol.Oth.10.AllAg.Olfactory_epithelium [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.10.AllAg.Olfactory_epithelium mm9 RNA polymerase Others Olfactory epithelium... SRX112963,SRX143827 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.10.AllAg.Olfactory_epithelium.bed ...

  20. File list: Pol.Oth.05.AllAg.Olfactory_epithelium [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.05.AllAg.Olfactory_epithelium mm9 RNA polymerase Others Olfactory epithelium... SRX143827,SRX112963 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.05.AllAg.Olfactory_epithelium.bed ...

  1. File list: Pol.Oth.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  2. File list: Pol.Myo.05.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Myo.05.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Muscle SR.../dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Myo.05.RNA_Polymerase_II.AllCell.bed ...

  3. File list: Pol.YSt.10.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.YSt.10.RNA_Polymerase_II.AllCell sacCer3 RNA polymerase RNA Polymerase II Yeast... strain SRX092435,SRX360917,SRX360914,SRX497380,SRX497382,SRX497381,SRX360915 http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Pol.YSt.10.RNA_Polymerase_II.AllCell.bed ...

  4. File list: Pol.Lar.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Lar.05.RNA_polymerase_II.AllCell dm3 RNA polymerase RNA polymerase II Larvae SR...SRX151962,SRX182775,SRX661503,SRX013070,SRX013072,SRX013113,SRX013082,SRX151961 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Lar.05.RNA_polymerase_II.AllCell.bed ...

  5. File list: Pol.CDV.10.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.10.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Cardiova...,SRX080152,SRX080153,SRX367018,SRX367016 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.CDV.10.RNA_polymerase_II.AllCell.bed ...

  6. File list: Pol.Lar.05.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  7. File list: Pol.Bld.20.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bld.20.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Blood SRX...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Bld.20.RNA_Polymerase_II.AllCell.bed ...

  8. File list: Pol.Epd.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Epd.50.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Epidermi...247,SRX080162,SRX807622 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Epd.50.RNA_polymerase_II.AllCell.bed ...

  9. File list: Pol.Lng.10.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Lng.10.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Lung SRX1...43816,SRX062976,SRX020252 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Lng.10.RNA_Polymerase_II.AllCell.bed ...

  10. File list: Pol.Emb.10.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.10.RNA_polymerase_II.AllCell ce10 RNA polymerase RNA polymerase II Embryo S...,SRX043867 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Emb.10.RNA_polymerase_II.AllCell.bed ...

  11. File list: Pol.Utr.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.05.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Uterus S...SRX573070,SRX027921,SRX1048949,SRX1136641,SRX1136638,SRX099217 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.05.RNA_polymerase_II.AllCell.bed ...

  12. File list: Pol.Neu.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.20.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Neural S...6,SRX743838,SRX743832,SRX743834,SRX743840 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Neu.20.RNA_polymerase_II.AllCell.bed ...

  13. File list: Pol.Plc.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Plc.50.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Placenta... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Plc.50.RNA_polymerase_II.AllCell.bed ...

  14. File list: Pol.Oth.05.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.05.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Others SR...X143827,SRX112963,SRX736456,SRX736457,SRX112981,SRX143834,SRX335666,SRX957689 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.05.RNA_Polymerase_II.AllCell.bed ...

  15. File list: Pol.Emb.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.20.RNA_polymerase_II.AllCell ce10 RNA polymerase RNA polymerase II Embryo S...,SRX043869 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Emb.20.RNA_polymerase_II.AllCell.bed ...

  16. File list: Pol.Gon.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Gon.20.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Gonad ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Gon.20.RNA_polymerase_II.AllCell.bed ...

  17. File list: Pol.Unc.05.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.05.RNA_Polymerase_II.AllCell ce10 RNA polymerase RNA Polymerase II Unclassi...fied http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Unc.05.RNA_Polymerase_II.AllCell.bed ...

  18. File list: Pol.Adp.50.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adp.50.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Adipocyte... SRX800011,SRX800010,SRX341031,SRX341032,SRX341029,SRX800016,SRX800017,SRX341030 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Adp.50.RNA_Polymerase_II.AllCell.bed ...

  19. File list: Pol.ALL.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.05.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II All cell...,SRX1013886,SRX1013900 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.ALL.05.RNA_polymerase_II.AllCell.bed ...

  20. File list: Pol.CDV.20.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.20.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Cardiovas...X320034,SRX346170,SRX346169,SRX373605,SRX680476 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.CDV.20.RNA_Polymerase_II.AllCell.bed ...

  1. File list: Pol.Unc.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.05.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Unclassi...fied http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Unc.05.RNA_polymerase_II.AllCell.bed ...

  2. File list: Pol.Spl.10.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Spl.10.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Spleen SR...X062981,SRX143838,SRX020253 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Spl.10.RNA_Polymerase_II.AllCell.bed ...

  3. File list: Pol.Unc.05.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.05.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Unclassif...ied SRX254629 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Unc.05.RNA_Polymerase_II.AllCell.bed ...

  4. File list: Pol.ALL.20.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.20.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II All cell ...//dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.ALL.20.RNA_Polymerase_II.AllCell.bed ...

  5. File list: Pol.Unc.10.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.10.RNA_Polymerase_II.AllCell ce10 RNA polymerase RNA Polymerase II Unclassi...fied http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Unc.10.RNA_Polymerase_II.AllCell.bed ...

  6. File list: Pol.Neu.20.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.20.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Neural SR...,SRX685285,SRX217736 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.20.RNA_Polymerase_II.AllCell.bed ...

  7. File list: Pol.Bld.50.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bld.50.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Blood SRX...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Bld.50.RNA_Polymerase_II.AllCell.bed ...

  8. File list: Pol.Pup.10.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Pup.10.RNA_polymerase_II.AllCell dm3 RNA polymerase RNA polymerase II Pupae SRX...013069 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Pup.10.RNA_polymerase_II.AllCell.bed ...

  9. File list: Pol.Emb.50.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.50.RNA_Polymerase_II.AllCell ce10 RNA polymerase RNA Polymerase II Embryo h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Emb.50.RNA_Polymerase_II.AllCell.bed ...

  10. File list: Pol.Unc.20.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.20.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Unclassif...ied SRX254629 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Unc.20.RNA_Polymerase_II.AllCell.bed ...

  11. File list: Pol.Lng.05.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Lng.05.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Lung SRX0...62976,SRX143816,SRX020252 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Lng.05.RNA_Polymerase_II.AllCell.bed ...

  12. File list: Pol.Lng.20.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Lng.20.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Lung SRX0...62976,SRX143816,SRX020252 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Lng.20.RNA_Polymerase_II.AllCell.bed ...

  13. File list: Pol.Gon.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Gon.50.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Gonad ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Gon.50.RNA_polymerase_II.AllCell.bed ...

  14. File list: Pol.Kid.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Kid.20.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Kidney S...SRX1206072,SRX1206066,SRX326423,SRX1206067,SRX003883,SRX003882,SRX367323 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Kid.20.RNA_polymerase_II.AllCell.bed ...

  15. File list: Pol.Adl.05.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.05.RNA_Polymerase_II.AllCell ce10 RNA polymerase RNA Polymerase II Adult SR...SRX1388757,SRX1388756 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Adl.05.RNA_Polymerase_II.AllCell.bed ...

  16. File list: Pol.PSC.50.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Pluripote...SRX213760,SRX213764 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.RNA_Polymerase_II.AllCell.bed ...

  17. File list: Pol.Epd.10.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Epd.10.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Epidermi...246,SRX663247,SRX807622 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Epd.10.RNA_polymerase_II.AllCell.bed ...

  18. File list: Pol.Epd.10.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Epd.10.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Epidermis... http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Epd.10.RNA_Polymerase_II.AllCell.bed ...

  19. File list: Pol.Brs.10.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Brs.10.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Breast SR...078990 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Brs.10.RNA_Polymerase_II.AllCell.bed ...

  20. File list: Pol.Dig.20.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.20.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Digestive... tract SRX112957,SRX143802 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.20.RNA_Polymerase_II.AllCell.bed ...

  1. File list: Pol.Spl.05.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Spl.05.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Spleen SR...X062981,SRX143838,SRX020253 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Spl.05.RNA_Polymerase_II.AllCell.bed ...

  2. File list: Pol.PSC.20.RNA_Polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.RNA_Polymerase_II.AllCell mm9 RNA polymerase RNA Polymerase II Pluripote...SRX213760,SRX213764 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.RNA_Polymerase_II.AllCell.bed ...

  3. File list: Pol.Pup.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Pup.05.RNA_polymerase_II.AllCell dm3 RNA polymerase RNA polymerase II Pupae SRX...013069 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Pup.05.RNA_polymerase_II.AllCell.bed ...

  4. File list: Pol.Unc.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.50.RNA_polymerase_II.AllCell ce10 RNA polymerase RNA polymerase II Unclassi...p://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Unc.50.RNA_polymerase_II.AllCell.bed ...

  5. File list: Pol.Plc.10.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Plc.10.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Placenta... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Plc.10.RNA_polymerase_II.AllCell.bed ...

  6. File list: Pol.Unc.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.05.RNA_polymerase_II.AllCell dm3 RNA polymerase RNA polymerase II Unclassif...ied SRX110774 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Unc.05.RNA_polymerase_II.AllCell.bed ...

  7. File list: Pol.ALL.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.05.RNA_polymerase_II.AllCell dm3 RNA polymerase RNA polymerase II All cell ...013077,SRX050604,SRX050605 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.ALL.05.RNA_polymerase_II.AllCell.bed ...

  8. File list: Pol.Adp.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adp.50.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Adipocyt...e SRX682084,SRX682086,SRX682085,SRX682083 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Adp.50.RNA_polymerase_II.AllCell.bed ...

  9. File list: Pol.ALL.10.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  10. File list: Pol.ALL.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.05.RNA_polymerase_II.AllCell sacCer3 RNA polymerase RNA polymerase II All c...ell types http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Pol.ALL.05.RNA_polymerase_II.AllCell.bed ...

  11. File list: Pol.Emb.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  12. File list: Pol.Epd.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Epd.20.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Epidermi...248,SRX663247,SRX807622 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Epd.20.RNA_polymerase_II.AllCell.bed ...

  13. File list: Pol.Plc.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Plc.05.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Placenta... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Plc.05.RNA_polymerase_II.AllCell.bed ...

  14. File list: Pol.Unc.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Unc.20.RNA_polymerase_II.AllCell ce10 RNA polymerase RNA polymerase II Unclassi...p://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Unc.20.RNA_polymerase_II.AllCell.bed ...

  15. File list: Pol.Kid.10.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Kid.10.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Kidney S...X1206068,SRX1206073,SRX1206074,SRX1206072,SRX1206071,SRX003882,SRX367323 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Kid.10.RNA_polymerase_II.AllCell.bed ...

  16. File list: Pol.Adl.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.50.RNA_polymerase_II.AllCell ce10 RNA polymerase RNA polymerase II Adult SR...SRX043965,SRX005629,SRX043964,SRX554718 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Adl.50.RNA_polymerase_II.AllCell.bed ...

  17. File list: Pol.Prs.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Prs.50.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Prostate...932,SRX020922,SRX022582 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Prs.50.RNA_polymerase_II.AllCell.bed ...

  18. File list: Pol.PSC.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Pluripot...670820,SRX702057,SRX702061 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.20.RNA_polymerase_II.AllCell.bed ...

  19. File list: Pol.Utr.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.50.RNA_polymerase_II.AllCell hg19 RNA polymerase RNA polymerase II Uterus S...RX099218,SRX1136641,SRX1048949,SRX1136639,SRX665233,SRX1136638 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.50.RNA_polymerase_II.AllCell.bed ...

  20. File list: Pol.Adl.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.20.RNA_polymerase_II.AllCell ce10 RNA polymerase RNA polymerase II Adult SR...SRX554718,SRX043965,SRX043963,SRX043964 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Adl.20.RNA_polymerase_II.AllCell.bed ...

  1. File list: Pol.Dig.50.AllAg.Intestinal_villus [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.50.AllAg.Intestinal_villus mm9 RNA polymerase Digestive tract Intestinal vi...llus http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.50.AllAg.Intestinal_villus.bed ...

  2. File list: Pol.Dig.10.AllAg.Intestines [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.10.AllAg.Intestines mm9 RNA polymerase Digestive tract Intestines SRX112957...,SRX143802 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.10.AllAg.Intestines.bed ...

  3. File list: Pol.Dig.50.AllAg.Intestine,_Small [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.50.AllAg.Intestine,_Small hg19 RNA polymerase Digestive tract Intestine, Sm...all http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Dig.50.AllAg.Intestine,_Small.bed ...

  4. File list: Pol.Dig.10.AllAg.Intestine,_Small [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.10.AllAg.Intestine,_Small hg19 RNA polymerase Digestive tract Intestine, Sm...all http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Dig.10.AllAg.Intestine,_Small.bed ...

  5. File list: Pol.Dig.10.AllAg.Intestinal_crypt [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.10.AllAg.Intestinal_crypt mm9 RNA polymerase Digestive tract Intestinal cry...pt http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.10.AllAg.Intestinal_crypt.bed ...

  6. File list: Pol.Dig.20.AllAg.Intestine,_Small [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.20.AllAg.Intestine,_Small hg19 RNA polymerase Digestive tract Intestine, Sm...all http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Dig.20.AllAg.Intestine,_Small.bed ...

  7. File list: Pol.Dig.20.AllAg.Intestines [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.20.AllAg.Intestines mm9 RNA polymerase Digestive tract Intestines SRX112957...,SRX143802 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.20.AllAg.Intestines.bed ...

  8. File list: Pol.Dig.05.AllAg.Intestines [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.05.AllAg.Intestines mm9 RNA polymerase Digestive tract Intestines SRX112957...,SRX143802 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.05.AllAg.Intestines.bed ...

  9. File list: Pol.Dig.50.AllAg.Intestinal_adenoma [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.50.AllAg.Intestinal_adenoma mm9 RNA polymerase Digestive tract Intestinal a...denoma http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Dig.50.AllAg.Intestinal_adenoma.bed ...

  10. File list: Pol.Emb.10.AllAg.Mixed_embryo [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.10.AllAg.Mixed_embryo ce10 RNA polymerase Embryo Mixed embryo SRX208771,SRX...208773,SRX208772,SRX208774 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Emb.10.AllAg.Mixed_embryo.bed ...

  11. File list: Pol.Emb.10.AllAg.Early_embryo [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.10.AllAg.Early_embryo ce10 RNA polymerase Embryo Early embryo SRX495119,SRX...495120,SRX043866,SRX043864,SRX043865,SRX043863 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Emb.10.AllAg.Early_embryo.bed ...

  12. File list: Pol.ALL.10.Polr3d.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.10.Polr3d.AllCell mm9 RNA polymerase Polr3d All cell types SRX373040,SRX301...04147 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.ALL.10.Polr3d.AllCell.bed ...

  13. File list: Pol.ALL.05.Polr3d.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.05.Polr3d.AllCell mm9 RNA polymerase Polr3d All cell types SRX373040,SRX373...04148 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.ALL.05.Polr3d.AllCell.bed ...

  14. File list: Pol.Neu.50.AllAg.Nerve_Sheath_Neoplasms [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.50.AllAg.Nerve_Sheath_Neoplasms mm9 RNA polymerase Neural Nerve Sheath Neop...lasms http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.50.AllAg.Nerve_Sheath_Neoplasms.bed ...

  15. File list: Pol.Neu.20.AllAg.Nerve_Sheath_Neoplasms [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.20.AllAg.Nerve_Sheath_Neoplasms mm9 RNA polymerase Neural Nerve Sheath Neop...lasms http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.20.AllAg.Nerve_Sheath_Neoplasms.bed ...

  16. File list: Pol.Neu.10.AllAg.Nerve_Sheath_Neoplasms [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.10.AllAg.Nerve_Sheath_Neoplasms mm9 RNA polymerase Neural Nerve Sheath Neop...lasms http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.10.AllAg.Nerve_Sheath_Neoplasms.bed ...

  17. File list: Pol.Neu.05.AllAg.Nerve_Sheath_Neoplasms [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Neu.05.AllAg.Nerve_Sheath_Neoplasms mm9 RNA polymerase Neural Nerve Sheath Neop...lasms http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Neu.05.AllAg.Nerve_Sheath_Neoplasms.bed ...

  18. File list: Pol.CDV.05.AllAg.Endocardial_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.05.AllAg.Endocardial_cells hg19 RNA polymerase Cardiovascular Endocardial c...ells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.CDV.05.AllAg.Endocardial_cells.bed ...

  19. File list: Pol.CDV.50.AllAg.Endocardial_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.50.AllAg.Endocardial_cells hg19 RNA polymerase Cardiovascular Endocardial c...ells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.CDV.50.AllAg.Endocardial_cells.bed ...

  20. File list: Pol.CDV.20.AllAg.Endocardial_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.20.AllAg.Endocardial_cells hg19 RNA polymerase Cardiovascular Endocardial c...ells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.CDV.20.AllAg.Endocardial_cells.bed ...

  1. File list: Pol.CDV.10.AllAg.Endocardial_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.CDV.10.AllAg.Endocardial_cells hg19 RNA polymerase Cardiovascular Endocardial c...ells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.CDV.10.AllAg.Endocardial_cells.bed ...

  2. File list: Pol.Gon.05.AllAg.Testicular_somatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Gon.05.AllAg.Testicular_somatic_cells mm9 RNA polymerase Gonad Testicular somatic... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Gon.05.AllAg.Testicular_somatic_cells.bed ...

  3. File list: Pol.Gon.20.AllAg.Testicular_somatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Gon.20.AllAg.Testicular_somatic_cells mm9 RNA polymerase Gonad Testicular somatic... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Gon.20.AllAg.Testicular_somatic_cells.bed ...

  4. File list: Pol.Gon.10.AllAg.Testicular_somatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Gon.10.AllAg.Testicular_somatic_cells mm9 RNA polymerase Gonad Testicular somatic... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Gon.10.AllAg.Testicular_somatic_cells.bed ...

  5. File list: Pol.PSC.10.AllAg.iPSC_intermediates [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.iPSC_intermediates mm9 RNA polymerase Pluripotent stem cell iPSC intermediates...e.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.iPSC_intermediates.bed ...

  6. File list: Pol.PSC.05.AllAg.iPSC_intermediates [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.iPSC_intermediates mm9 RNA polymerase Pluripotent stem cell iPSC intermediates...e.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.iPSC_intermediates.bed ...

  7. File list: Pol.PSC.20.AllAg.iPSC_intermediates [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.iPSC_intermediates mm9 RNA polymerase Pluripotent stem cell iPSC intermediates...e.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.iPSC_intermediates.bed ...

  8. File list: Pol.PSC.50.AllAg.iPSC_intermediates [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.iPSC_intermediates mm9 RNA polymerase Pluripotent stem cell iPSC intermediates...e.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.iPSC_intermediates.bed ...

  9. File list: Pol.Adl.05.AllAg.Young_adult [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.05.AllAg.Young_adult ce10 RNA polymerase Adult Young adult SRX395531,SRX331...RX043965 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Adl.05.AllAg.Young_adult.bed ...

  10. File list: Pol.Adl.10.AllAg.Young_adult [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.10.AllAg.Young_adult ce10 RNA polymerase Adult Young adult SRX395531,SRX331...RX043965 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Adl.10.AllAg.Young_adult.bed ...

  11. File list: Pol.Adl.20.AllAg.Young_adult [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Adl.20.AllAg.Young_adult ce10 RNA polymerase Adult Young adult SRX331268,SRX331...RX043964 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/assembled/Pol.Adl.20.AllAg.Young_adult.bed ...

  12. File list: Pol.Oth.50.AllAg.Pancreas_and_brain [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.50.AllAg.Pancreas_and_brain mm9 RNA polymerase Others Pancreas and brain ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.50.AllAg.Pancreas_and_brain.bed ...

  13. File list: Pol.Oth.20.AllAg.Pancreas_and_brain [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.20.AllAg.Pancreas_and_brain mm9 RNA polymerase Others Pancreas and brain ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.20.AllAg.Pancreas_and_brain.bed ...

  14. File list: Pol.Oth.10.AllAg.Pancreas_and_brain [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.10.AllAg.Pancreas_and_brain mm9 RNA polymerase Others Pancreas and brain ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.10.AllAg.Pancreas_and_brain.bed ...

  15. File list: Pol.Oth.05.AllAg.Pancreas_and_brain [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Oth.05.AllAg.Pancreas_and_brain mm9 RNA polymerase Others Pancreas and brain ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Oth.05.AllAg.Pancreas_and_brain.bed ...

  16. File list: Pol.Emb.20.AllAg.Neuronal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.20.AllAg.Neuronal_cells dm3 RNA polymerase Embryo Neuronal cells SRX110756 ...http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Emb.20.AllAg.Neuronal_cells.bed ...

  17. File list: Pol.Emb.50.AllAg.Neuronal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.50.AllAg.Neuronal_cells dm3 RNA polymerase Embryo Neuronal cells SRX110756 ...http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Emb.50.AllAg.Neuronal_cells.bed ...

  18. File list: Pol.Emb.05.AllAg.Neuronal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.05.AllAg.Neuronal_cells dm3 RNA polymerase Embryo Neuronal cells SRX110756 ...http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Emb.05.AllAg.Neuronal_cells.bed ...

  19. File list: Pol.Brs.20.AllAg.Mammary_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Brs.20.AllAg.Mammary_cells mm9 RNA polymerase Breast Mammary cells SRX852566,SR...X852567,SRX187510,SRX187515 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Brs.20.AllAg.Mammary_cells.bed ...

  20. File list: Pol.Emb.10.AllAg.Neuronal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Emb.10.AllAg.Neuronal_cells dm3 RNA polymerase Embryo Neuronal cells SRX110756 ...http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.Emb.10.AllAg.Neuronal_cells.bed ...