WorldWideScience

Sample records for plate count analysis

  1. Multiregional evaluation of the SimPlate heterotrophic plate count method compared to the standard plate count agar pour plate method in water.

    Science.gov (United States)

    Jackson, R W; Osborne, K; Barnes, G; Jolliff, C; Zamani, D; Roll, B; Stillings, A; Herzog, D; Cannon, S; Loveland, S

    2000-01-01

    A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35 degrees C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0. 95; y = 0.99X + 0.06).

  2. Multiregional Evaluation of the SimPlate Heterotrophic Plate Count Method Compared to the Standard Plate Count Agar Pour Plate Method in Water

    OpenAIRE

    Jackson, R. Wayne; Osborne, Karen; Barnes, Gary; Jolliff, Carol; Zamani, Dianna; Roll, Bruce; Stillings, Amy; Herzog, David; Cannon, Shelly; Loveland, Scott

    2000-01-01

    A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35°C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0.95; y = 0.99X + 0.06).

  3. Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis

    Institute of Scientific and Technical Information of China (English)

    LI Xinyu; ZHANG Huiwen; WU Minna; ZHANG Yan; ZHANG Chenggang

    2008-01-01

    Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels (50, 150, and 250 mg/kg) were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos (250 mg/kg) could significantly stimulate fungal populations. DCGE (denaturing gradient gel electrophoresis) fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak.

  4. Heterotrophic plate count methodology in the United States.

    Science.gov (United States)

    Reasoner, Donald J

    2004-05-01

    In the United States (US), the history of bacterial plate counting (BPC) methods used for water can be traced largely through Standard Methods for the Examination of Water and Wastewater (Standard Methods). The bacterial count method has evolved from the original Standard Methods (1st edition, 1905) plate count which used nutrient gelatin and incubation at 20 degrees C for 48 h, to the HPC method options in the latest edition of Standard Methods that provide greater flexibility of application, depending on the data needs of the water analyst. The use of agar-agar as a gelling agent, replacing gelatin, allowed the use of higher incubation temperatures and resulted in the "body temperature count" (37 degrees C) found in the 3rd through the 8th edition of Standard Methods. The change from 37 degrees C incubation to 35+/-0.5 degrees C accommodated laboratories that did both milk and water analyses. By using a single temperature, fewer incubators were needed. The term "standard plate count" (SPC) first appeared in 1960 (11th edition) along with plate count agar. Incubation at 20 degrees C for the plate count was dropped from the 13th to 15th editions and few changes were made in the SPC method from the 11th edition through the 13th editions. Plate count analysis of bottled waters was included in the 14th edition (1975), calling for incubation at 35+/-0.5 degrees C for 72+/-4 h. Perhaps the most significant changes in plate count methods occurred with the 16th edition (1985). The term heterotrophic plate count replaced the standard plate count, and the spread plate (SP) and membrane filter (MF) methods were added along with new media for pour and spread plates (R2A agar and NWRI agar, both low nutrient) and for the membrane filter method (mHPC medium). The use of low nutrient media, lower incubation temperature, and longer incubation times, results in higher plate count results for most water samples. The options currently available, including low and high nutrient media

  5. High counting rate resistive-plate chamber

    Science.gov (United States)

    Peskov, V.; Anderson, D. F.; Kwan, S.

    1993-05-01

    Parallel-plate avalanche chambers (PPAC) are widely used in physics experiments because they are fast (less than 1 ns) and have very simple construction: just two parallel metallic plates or mesh electrodes. Depending on the applied voltage they may work either in spark mode or avalanche mode. The advantage of the spark mode of operation is a large signal amplitude from the chamber, the disadvantage is that there is a large dead time (msec) for the entire chamber after an event. The main advantage of the avalanche mode is high rate capability 10(exp 5) counts/mm(sup 2). A resistive-plate chamber (RPC) is similar to the PPAC in construction except that one or both of the electrodes are made from high resistivity (greater than 10(exp 10) Omega(cm) materials. In practice RPC's are usually used in the spark mode. Resistive electrodes are charged by sparks, locally reducing the actual electric field in the gap. The size of the charged surface is about 10 mm(sup 2), leaving the rest of the detector unaffected. Therefore, the rate capability of such detectors in the spark mode is considerably higher than conventional spark counters. Among the different glasses tested the best results were obtained with electron type conductive glasses, which obey Ohm's law. Most of the work with such glasses was done with high pressure parallel-plate chambers (10 atm) for time-of-flight measurements. Resistive glasses have been expensive and produced only in small quantities. Now resistive glasses are commercially available, although they are still expensive in small scale production. From the positive experience of different groups working with the resistive glasses, it was decided to review the old idea to use this glass for the RPC. This work has investigated the possibility of using the RPC at 1 atm and in the avalanche mode. This has several advantages: simplicity of construction, high rate capability, low voltage operation, and the ability to work with non-flammable gases.

  6. Repeatability of differential goat bulk milk culture and associations with somatic cell count, total bacterial count, and standard plate count

    OpenAIRE

    Koop, G.; Dik, N; Nielen, M; Lipman, L. J. A.

    2010-01-01

    The aims of this study were to assess how different bacterial groups in bulk milk are related to bulk milk somatic cell count (SCC), bulk milk total bacterial count (TBC), and bulk milk standard plate count (SPC) and to measure the repeatability of bulk milk culturing. On 53 Dutch dairy goat farms, 3 bulk milk samples were collected at intervals of 2 wk. The samples were cultured for SPC, coliform count, and staphylococcal count and for the presence of Staphylococcus aureus. Furthermore, SCC ...

  7. Repeatability of differential goat bulk milk culture and associations with somatic cell count, total bacterial count, and standard plate count

    NARCIS (Netherlands)

    Koop, G.; Dik, N.; Nielen, M.; Lipman, L.J.A.

    2010-01-01

    The aims of this study were to assess how different bacterial groups in bulk milk are related to bulk milk somatic cell count (SCC), bulk milk total bacterial count (TBC), and bulk milk standard plate count (SPC) and to measure the repeatability of bulk milk culturing. On 53 Dutch dairy goat farms,

  8. HETEROTROPHIC PLATE COUNT (HPC) METHODOLOGY IN THE UNITED STATES

    Science.gov (United States)

    ABSTRACT In the United States (U.S.), the history of bacterial plate counting methods used for water can be traced largely through Standard Methods for the Examination of Water and Wastewater (Standard Methods). The bacterial count method has evolved from the original St...

  9. A THUMBNAIL HISTORY OF HETEROTROPHIC PLATE COUNT (HPC) METHODOLOGY IN THE UNITED STATES

    Science.gov (United States)

    Over the past 100 years, the method of determining the number of bacteria in water, foods or other materials has been termed variously as: bacterial plate count, total plate count, total viable plate count, aerobic plate count, standard plate cound and more recently, heterotrophi...

  10. Evaluation of DAPI direct count, computer assisted and plate count methods

    OpenAIRE

    Chivu, Bogdan

    2010-01-01

    The feasibility of using automatic counting of bacteria stained with highly specific and sensitive fluorescing DNA stain DAPI, 4',6-diamidino-2-phenylindole, and direct manual counting to enumerate both pure culture of Pseudomonas putida overnight culture and sea water enhanced culture, was tested in correlation with plate direct counting, turbidity and absorbance at 600nm, to obtain cross validation. Six diluted samples from overnight pure culture of Pseudomonas putida and sea water culture ...

  11. A new medium for determining the total plate count in food.

    Science.gov (United States)

    Smith, C F; Townsend, D E

    1999-12-01

    SimPlate for Total Plate Count-Color Indicator (TPC-CI, IDEXX Laboratories, Inc., Westbrook, Me.) is a new medium that incorporates the redox dye resazurin to detect and quantify bacteria in food. Enumeration is achieved by the most probable number method using a SimPlate device. Viable bacteria are detected in each well of the SimPlate device by the biochemical reduction of resazurin, which is blue, to the pink resorufin or the clear dihydroresorufin indicators. Results after 24 h of incubation for TPC-CI are highly correlated with standard plate count agar after 48 h of incubation. Correlation coefficients from studies conducted at five laboratories ranged from 0.94 to 0.98 in side-by-side comparisons against standard plate count agar. Four additional test sites, using alternative methods for determining the aerobic plate count in food, reported similar results in comparison studies (r = 0.91 to 0.97). The slopes from linear regression analysis at all sites ranged from 0.91 to 0.98, with y intercepts ranging from 0.11 to 0.84. Samples used for the validation of TPC-CI included raw food products (i.e., liver and grains), which may contain natural enzymes that interfere with enzyme-based detection methods. No interference was seen from the foods tested. These results suggest that TPC-CI is a suitable alternative to existing plate count methods and has reduced incubation time.

  12. Collaborative study comparing the spiral plate and aerobic plate count methods.

    Science.gov (United States)

    Gilchrist, J E; Donnelly, C B; Peeler, J T; Campbell, J E

    1977-07-01

    The spiral plate count method is a semiautomated plating technique that greatly reduces manpower and material costs normally associated with the pour plating technique. In this collaborative study, 8 laboratories compared the spiral and pour plating techniques, using 4 samples each of 3 products: frozen pumpkin pie, frozen chicken pot pie, and shampoo. The results show that 10 of the 12 comparisons of means of the pour and spiral methods were not significantly different; 2 values were significant at alpha = 0.01. Overall, the components of variance were less than that of the current milk standard, and the replicate per cent coefficient of variation was satisfactory. This study indicates that the spiral plate method is an acceptable alternative to the pour plate method; the spiral plate method has been adopted as official first action.

  13. Correlation between standard plate count and somatic cell count milk quality results for Wisconsin dairy producers.

    Science.gov (United States)

    Borneman, Darand L; Ingham, Steve

    2014-05-01

    The objective of this study was to determine if a correlation exists between standard plate count (SPC) and somatic cell count (SCC) monthly reported results for Wisconsin dairy producers. Such a correlation may indicate that Wisconsin producers effectively controlling sanitation and milk temperature (reflected in low SPC) also have implemented good herd health management practices (reflected in low SCC). The SPC and SCC results for all grade A and B dairy producers who submitted results to the Wisconsin Department of Agriculture, Trade, and Consumer Protection, in each month of 2012 were analyzed. Grade A producer SPC results were less dispersed than grade B producer SPC results. Regression analysis showed a highly significant correlation between SPC and SCC, but the R(2) value was very small (0.02-0.03), suggesting that many other factors, besides SCC, influence SPC. Average SCC (across 12 mo) for grade A and B producers decreased with an increase in the number of monthly SPC results (out of 12) that were ≤ 25,000 cfu/mL. A chi-squared test of independence showed that the proportion of monthly SCC results >250,000 cells/mL varied significantly depending on whether the corresponding SPC result was ≤ 25,000 or >25,000 cfu/mL. This significant difference occurred in all months of 2012 for grade A and B producers. The results suggest that a generally consistent level of skill exists across dairy production practices affecting SPC and SCC.

  14. 进口葡萄酒菌落总数检验结果分析%Analysis on the results of aerobic plate count in imported wine

    Institute of Scientific and Technical Information of China (English)

    周毅; 李金华; 徐晔; 段宏安; 曹洁

    2013-01-01

    目的:了解进口葡萄酒微生物污染状况及主要污染微生物.方法:对394份进口葡萄酒样品菌落总数进行测定,并对主要污染微生物进行鉴定.结果:5份葡萄酒样品菌落总数超标,生化鉴定表明,主要污染微生物为酿酒酵母.结论:进口葡萄酒整体卫生状况较好,但进出口管理部门应加强对进口葡萄酒产品微生物指标的检测,确保产品质量符合相关标准要求.%Objective:A survey was conducted to determine the prevalence of microbiological contamination in im-ported wine,and find the main microbe leading to pollution.Methods:A total of 394 imported wine samples were analyzed for aerobic plate count determination,then microbial identification was applied to these contaminated microbes.Results:Aerobic plate count of 5 wine samples was found over-limit.The results of biochemical identification showed that the main microorganism causing pollution is Saccharomyces cerevisiae.Conclusion:The overall prevalence of microbiological contamination in wine is relatively low,but the import and export administrative department still should strengthen the microbiological test on imported wine,to make sure that the product quality can meet revelant standards requirement.

  15. Flow cytometric bacterial cell counts challenge conventional heterotrophic plate counts for routine microbiological drinking water monitoring

    KAUST Repository

    Van Nevel, S.

    2017-02-08

    Drinking water utilities and researchers continue to rely on the century-old heterotrophic plate counts (HPC) method for routine assessment of general microbiological water quality. Bacterial cell counting with flow cytometry (FCM) is one of a number of alternative methods that challenge this status quo and provide an opportunity for improved water quality monitoring. After more than a decade of application in drinking water research, FCM methodology is optimised and established for routine application, supported by a considerable amount of data from multiple full-scale studies. Bacterial cell concentrations obtained by FCM enable quantification of the entire bacterial community instead of the minute fraction of cultivable bacteria detected with HPC (typically < 1% of all bacteria). FCM measurements are reproducible with relative standard deviations below 3% and can be available within 15 min of samples arriving in the laboratory. High throughput sample processing and complete automation are feasible and FCM analysis is arguably less expensive than HPC when measuring more than 15 water samples per day, depending on the laboratory and selected staining procedure(s). Moreover, many studies have shown FCM total (TCC) and intact (ICC) cell concentrations to be reliable and robust process variables, responsive to changes in the bacterial abundance and relevant for characterising and monitoring drinking water treatment and distribution systems. The purpose of this critical review is to initiate a constructive discussion on whether FCM could replace HPC in routine water quality monitoring. We argue that FCM provides a faster, more descriptive and more representative quantification of bacterial abundance in drinking water.

  16. Optimization of high count rate event counting detector with Microchannel Plates and quad Timepix readout

    Energy Technology Data Exchange (ETDEWEB)

    Tremsin, A.S., E-mail: ast@ssl.berkeley.edu; Vallerga, J.V.; McPhate, J.B.; Siegmund, O.H.W.

    2015-07-01

    Many high resolution event counting devices process one event at a time and cannot register simultaneous events. In this article a frame-based readout event counting detector consisting of a pair of Microchannel Plates and a quad Timepix readout is described. More than 10{sup 4} simultaneous events can be detected with a spatial resolution of ~55 µm, while >10{sup 3} simultaneous events can be detected with <10 µm spatial resolution when event centroiding is implemented. The fast readout electronics is capable of processing >1200 frames/sec, while the global count rate of the detector can exceed 5×10{sup 8} particles/s when no timing information on every particle is required. For the first generation Timepix readout, the timing resolution is limited by the Timepix clock to 10–20 ns. Optimization of the MCP gain, rear field voltage and Timepix threshold levels are crucial for the device performance and that is the main subject of this article. These devices can be very attractive for applications where the photon/electron/ion/neutron counting with high spatial and temporal resolution is required, such as energy resolved neutron imaging, Time of Flight experiments in lidar applications, experiments on photoelectron spectroscopy and many others.

  17. Hydrophobic grid membrane filter method for aerobic plate count in foods: collaborative study.

    Science.gov (United States)

    Entis, P

    1986-01-01

    Twenty-one laboratories participated in a collaborative study to validate a hydrophobic grid membrane filter (HGMF) method for aerobic plate count by comparing its performance against the AOAC/APHA pour plate method. Raw milk, raw poultry, whole egg powder, flours, and spices were included in the study. Counts obtained by the HGMF and pour plate methods did not differ significantly, except in the case of whole egg powder, for which the HGMF method produced significantly higher counts. The hydrophobic grid membrane filter method for aerobic plate count in foods has been adopted official first action.

  18. Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces--comparison with cell counting plates.

    Science.gov (United States)

    Djokic, Vitomir; Blaga, Radu; Rinaldi, Laura; Le Roux, Delphine; Ducry, Tamara; Maurelli, Maria Paola; Perret, Catherine; Djurkovic Djakovic, Olgica; Cringoli, Giuseppe; Boireau, Pascal

    2014-12-01

    Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 × 10(2) oocysts) compared to Kova Slide (5 × 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions.

  19. Flow cytometric bacterial cell counts challenge conventional heterotrophic plate counts for routine microbiological drinking water monitoring.

    Science.gov (United States)

    Van Nevel, S; Koetzsch, S; Proctor, C R; Besmer, M D; Prest, E I; Vrouwenvelder, J S; Knezev, A; Boon, N; Hammes, F

    2017-04-15

    Drinking water utilities and researchers continue to rely on the century-old heterotrophic plate counts (HPC) method for routine assessment of general microbiological water quality. Bacterial cell counting with flow cytometry (FCM) is one of a number of alternative methods that challenge this status quo and provide an opportunity for improved water quality monitoring. After more than a decade of application in drinking water research, FCM methodology is optimised and established for routine application, supported by a considerable amount of data from multiple full-scale studies. Bacterial cell concentrations obtained by FCM enable quantification of the entire bacterial community instead of the minute fraction of cultivable bacteria detected with HPC (typically water samples per day, depending on the laboratory and selected staining procedure(s). Moreover, many studies have shown FCM total (TCC) and intact (ICC) cell concentrations to be reliable and robust process variables, responsive to changes in the bacterial abundance and relevant for characterising and monitoring drinking water treatment and distribution systems. The purpose of this critical review is to initiate a constructive discussion on whether FCM could replace HPC in routine water quality monitoring. We argue that FCM provides a faster, more descriptive and more representative quantification of bacterial abundance in drinking water.

  20. HETEROTROPHIC PLATE COUNT BACTERIA - WHAT IS THEIR SIGNIFICANCE IN DRINKING WATER?

    Science.gov (United States)

    The possible health significance of heterotrophic plate count (HPC) bacteria, also know in earlier terminology as standard plate count (SPC) bacteria, in drinking water has been debated for decades. While the literature documents the universal occurrence of HPC bacteria in soil, ...

  1. Comparison of bulk-tank standard plate count and somatic cell count for Wisconsin dairy farms in three size categories.

    Science.gov (United States)

    Ingham, S C; Hu, Y; Ané, C

    2011-08-01

    The objective of this study was to evaluate possible claims by advocates of small-scale dairy farming that milk from smaller Wisconsin farms is of higher quality than milk from larger Wisconsin farms. Reported bulk tank standard plate count (SPC) and somatic cell count (SCC) test results for Wisconsin dairy farms were obtained for February to December, 2008. Farms were sorted into 3 size categories using available size-tracking criteria: small (≤118 cows; 12,866 farms), large (119-713 cattle; 1,565 farms), and confined animal feeding operations (≥714 cattle; 160 farms). Group means were calculated (group=farm size category) for the farms' minimum, median, mean, 90th percentile, and maximum SPC and SCC. Statistical analysis showed that group means for median, mean, 90th percentile, and maximum SPC and SCC were almost always significantly higher for the small farm category than for the large farm and confined animal feeding operations farm categories. With SPC and SCC as quality criteria and the 3 farm size categories of ≤118, 119 to 713, and ≥714 cattle, the claim of Wisconsin smaller farms producing higher quality milk than Wisconsin larger farms cannot be supported.

  2. Short communication: Repeatability of differential goat bulk milk culture and associations with somatic cell count, total bacterial count, and standard plate count.

    Science.gov (United States)

    Koop, G; Dik, N; Nielen, M; Lipman, L J A

    2010-06-01

    The aims of this study were to assess how different bacterial groups in bulk milk are related to bulk milk somatic cell count (SCC), bulk milk total bacterial count (TBC), and bulk milk standard plate count (SPC) and to measure the repeatability of bulk milk culturing. On 53 Dutch dairy goat farms, 3 bulk milk samples were collected at intervals of 2 wk. The samples were cultured for SPC, coliform count, and staphylococcal count and for the presence of Staphylococcus aureus. Furthermore, SCC (Fossomatic 5000, Foss, Hillerød, Denmark) and TBC (BactoScan FC 150, Foss) were measured. Staphylococcal count was correlated to SCC (r=0.40), TBC (r=0.51), and SPC (r=0.53). Coliform count was correlated to TBC (r=0.33), but not to any of the other variables. Staphylococcus aureus did not correlate to SCC. The contribution of the staphylococcal count to the SPC was 31%, whereas the coliform count comprised only 1% of the SPC. The agreement of the repeated measurements was low. This study indicates that staphylococci in goat bulk milk are related to SCC and make a significant contribution to SPC. Because of the high variation in bacterial counts, repeated sampling is necessary to draw valid conclusions from bulk milk culturing.

  3. Single ion counting with a MCP (microchannel plate) detector

    Energy Technology Data Exchange (ETDEWEB)

    Tawara, Hiroko; Sasaki, Shinichi; Miyajima, Mitsuhiro [National Lab. for High Energy Physics, Tsukuba, Ibaraki (Japan); Shibamura, Eido

    1996-07-01

    In this study, a single-ion-counting method using alpha-particle-impact ionization of Ar atoms is demonstrated and the preliminary {epsilon}{sub mcp} for Ar ions with incident energies of 3 to 4.7 keV is determined. The single-ion counting by the MCP is aimed to be performed under experimental conditions as follows: (1) A signal from the MCP is reasonably identified as incidence of single Ar-ion. (2) The counting rate of Ar ions is less than 1 s{sup -1}. (3) The incident Ar ions are not focused on a small part of an active area of the MCP, namely, {epsilon}{sub mcp} is determined with respect to the whole active area of the MCP. So far, any absolute detection efficiency has not been reported under these conditions. (J.P.N.)

  4. Spiral plate count method for the examination of raw and pasteurized milk.

    Science.gov (United States)

    Donnelly, C B; Gilchrist, J E; Peeler, J T; Campbell, J E

    1976-07-01

    The spiral plate count method (SPLPC) was compared with the standard plate count (SPC) method by examining 201 samples of raw and pasteurized milk. Although the means of the two methods differed significantly at alpha = 0.01,the difference was less than 10% and was not considered to be of any practical importance. The pooled replicate variances of both methods were less than 0.003, indicating good agreement between duplicate plates, with the variance of the SPLPC slightly less than that of the SPC. We believe this study indicates that the SPLPC could be substituted for the SPC in the bacteriological examination of milk.

  5. Comparison of Limulus assay, standard plate count, and total coliform count for microbiological assessment of renovated wastewater.

    Science.gov (United States)

    Jorgensen, J H; Lee, J C; Alexander, G A; Wolf, H W

    1979-05-01

    The Limulus endotoxin assay was compared to the standard plate count and total coliform count for assessment of the bacteriological quality of reclaimed wastewater. A total of 48 water samples from an advanced waste treatment plant in Dallas, Tex. were examined by the three techniques. Limulus assays were technically simpler to perform and provided results much sooner than conventional culture methods. However, the endotoxin values did not correlate extremely well with determinations of viable bacterial numbers. This lack of correlation may have been due to alterations in the normal ratio of viable gram-negative cells to endotoxin caused by water reclamation procedures.

  6. Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes

    Directory of Open Access Journals (Sweden)

    Behraad Bahreyni

    2013-06-01

    Full Text Available Development of a miniaturized biosensor system that can be used for rapid detection and counting of microorganisms in food or water samples is described. The developed microsystem employs a highly sensitive impedimetric array of biosensors to monitor the growth of bacterial colonies that are dispersed across an agar growth medium. To use the system, a sample containing the bacteria is cultured above the agar layer. Using a multiplexing network, the electrical properties of the medium at different locations are continuously measured, recorded, and compared against a baseline signal. Variations of signals from different biosensors are used to reveal the presence of bacteria in the sample, as well as the locations of bacterial colonies across the biochip. This technique forms the basis for a label-free bacterial detection for rapid analysis of food samples, reducing the detection time by at least a factor of four compared to the current required incubation times of 24 to 72 hours for plate count techniques. The developed microsystem has the potential for miniaturization to a stage where it could be deployed for rapid analysis of food samples at commercial scale at laboratories, food processing facilities, and retailers.

  7. Calibration of photon counting imaging microchannel plate detectors for EUV astronomy

    Science.gov (United States)

    Siegmund, O. H. W.; Vallerga, J.; Jelinsky, P.

    1986-01-01

    The calibration of photon counting imaging detectors for satellite based EUV astronomy is a complex process designed to ensure the validity of the data received 'in orbit'. The methods developed to accomplish calibration of microchannel plate detectors for the Extreme Ultraviolet Explorer are described and illustrated. The characterization of these detectors can be subdivided into three categories: stabilization, performance tests, and environmental tests.

  8. Atomic layer deposited borosilicate glass microchannel plates for large area event counting detectors

    Science.gov (United States)

    Siegmund, O. H. W.; McPhate, J. B.; Tremsin, A. S.; Jelinsky, S. R.; Hemphill, R.; Frisch, H. J.; Elam, J.; Mane, A.; Lappd Collaboration

    2012-12-01

    Borosilicate glass micro-capillary array substrates with 20 μm and 40 μm pores have been deposited with resistive, and secondary electron emissive, layers by atomic layer deposition to produce functional microchannel plates. Device formats of 32.7 mm and 20 cm square have been fabricated and tested in analog and photon counting modes. The tests show amplification, imaging, background rate, pulse shape and lifetime characteristics that are comparable to standard glass microchannel plates. Large area microchannel plates of this type facilitate the construction of 20 cm format sealed tube sensors with strip-line readouts that are being developed for Cherenkov light detection. Complementary work has resulted in Na2KSb bialkali photocathodes with peak quantum efficiency of 25% being made on borosilicate glass. Additionally GaN (Mg) opaque photocathodes have been successfully made on borosilicate microchannel plates.

  9. Atomic layer deposited borosilicate glass microchannel plates for large area event counting detectors

    Energy Technology Data Exchange (ETDEWEB)

    Siegmund, O.H.W., E-mail: ossy@ssl.berkeley.edu [Experimental Astrophysics Group, Space Sciences Laboratory, 7 Gauss Way, University of California, Berkeley, CA 94720 (United States); McPhate, J.B.; Tremsin, A.S.; Jelinsky, S.R.; Hemphill, R. [Experimental Astrophysics Group, Space Sciences Laboratory, 7 Gauss Way, University of California, Berkeley, CA 94720 (United States); Frisch, H.J. [Enrico Fermi Institute, 5640 S. Ellis Avenue University of Chicago, Chicago, IL 60637 (United States); Elam, J.; Mane, A. [Argonne National Laboratory, 9700 S. Cass Avenue Argonne, IL 60439 (United States); Collaboration: LAPPD Collaboration

    2012-12-11

    Borosilicate glass micro-capillary array substrates with 20 {mu}m and 40 {mu}m pores have been deposited with resistive, and secondary electron emissive, layers by atomic layer deposition to produce functional microchannel plates. Device formats of 32.7 mm and 20 cm square have been fabricated and tested in analog and photon counting modes. The tests show amplification, imaging, background rate, pulse shape and lifetime characteristics that are comparable to standard glass microchannel plates. Large area microchannel plates of this type facilitate the construction of 20 cm format sealed tube sensors with strip-line readouts that are being developed for Cherenkov light detection. Complementary work has resulted in Na{sub 2}KSb bialkali photocathodes with peak quantum efficiency of 25% being made on borosilicate glass. Additionally GaN (Mg) opaque photocathodes have been successfully made on borosilicate microchannel plates.

  10. Microbiological assessment of house and imported bottled water by comparison of bacterial endotoxin concentration, heterotrophic plate count, and fecal coliform count.

    Science.gov (United States)

    Reyes, Mayra I; Pérez, Cynthia M; Negrón, Edna L

    2008-03-01

    Consumers increasingly use bottled water and home water treatment systems to avoid direct tap water. According to the International Bottled Water Association (IBWA), an industry trade group, 5 billion gallons of bottled water were consumed by North Americans in 2001. The principal aim of this study was to assess the microbial quality of in-house and imported bottled water for human consumption, by measurement and comparison of the concentration of bacterial endotoxin and standard cultivable methods of indicator microorganisms, specifically, heterotrophic and fecal coliform plate counts. A total of 21 brands of commercial bottled water, consisting of 10 imported and 11 in-house brands, selected at random from 96 brands that are consumed in Puerto Rico, were tested at three different time intervals. The Standard Limulus Amebocyte Lysate test, gel clot method, was used to measure the endotoxin concentrations. The minimum endotoxin concentration in 63 water samples was less than 0.0625 EU/mL, while the maximum was 32 EU/mL. The minimum bacterial count showed no growth, while the maximum was 7,500 CFU/mL. Bacterial isolates like P. fluorescens, Corynebacterium sp. J-K, S. paucimobilis, P. versicularis, A. baumannii, P. chlororaphis, F. indologenes, A. faecalis and P. cepacia were identified. Repeated measures analysis of variance demonstrated that endotoxin concentration did not change over time, while there was a statistically significant (p count over time. In addition, multiple linear regression analysis demonstrated that a unit change in the concentration of endotoxin across time was associated with a significant (p count. This analysis evidenced a significant time effect in the average log bacteriological cell count. Although bacterial growth was not detected in some water samples, endotoxin was present. Measurement of Gram-negative bacterial endotoxins is one of the methods that have been suggested as a rapid way of determining bacteriological water quality.

  11. Evaluation of Petrifilm™ Select E. coli Count Plate medium to discriminate antimicrobial resistant Escherichia coli

    Directory of Open Access Journals (Sweden)

    Jensen Lars

    2008-09-01

    Full Text Available Abstract Background Screening and enumeration of antimicrobial resistant Escherichia coli directly from samples is needed to identify emerging resistant clones and obtain quantitative data for risk assessment. Aim of this study was to evaluate the performance of 3M™ Petrifilm™ Select E. coli Count Plate (SEC plate supplemented with antimicrobials to discriminate antimicrobial-resistant and non-resistant E. coli. Method A range of E. coli isolates were tested by agar dilution method comparing the Minimal Inhibitory Concentration (MIC for eight antimicrobials obtained by Mueller-Hinton II agar, MacConkey agar and SEC plates. Kappa statistics was used to assess the levels of agreement when classifying strains as resistant, intermediate or susceptible. Results SEC plate showed that 74% of all strains agreed within ± 1 log2 dilution when comparing MICs with Mueller-Hinton II media. High agreement levels were found for gentamicin, ampicillin, chloramphenicol and cefotaxime, resulting in a kappa value of 0.9 and 100% agreement within ± 1 log2 dilution. Significant variances were observed for oxytetracycline and sulphamethoxazole. Further tests showed that the observed discrepancy in classification of susceptibility to oxytetracycline by the two media could be overcome when a plate-dependent breakpoint of 64 mg/L was used for SEC plates. For sulphamethoxazole, SEC plates provided unacceptably high MICs. Conclusion SEC plates showed good agreement with Mueller-Hinton II agar in MIC studies and can be used to screen and discriminate resistant E. coli for ampicillin, cephalothin, streptomycin, chloramphenicol, cefotaxime and gentamicin using CLSI standardized breakpoints, but not for sulphamethoxazole. SEC plates can also be used to discriminate oxytetracycline-resistant E. coli if a plate-dependent breakpoint value of 64 mg/L is used.

  12. Analysis of bacterial diversity during the fermentation of inyu, a high-temperature fermented soy sauce, using nested PCR-denaturing gradient gel electrophoresis and the plate count method.

    Science.gov (United States)

    Wei, Chia-Li; Chao, Shiou-Huei; Tsai, Wen-Bin; Lee, Pei-Shan; Tsau, Nai-Hung; Chen, Jhih-Shan; Lai, Wen-Lin; Tu, James Ching-Yueh; Tsai, Ying-Chieh

    2013-04-01

    The diversity of bacteria associated with the fermentation of inyu, also known as black soy sauce, was studied through the nested PCR-denaturing gradient gel electrophoresis (DGGE) of samples collected from the fermentation stages of the inyu production process. The DGGE profiles targeted the bacterial 16S rDNA and revealed the presence of Citrobacter farmeri, Enterobacter cloacae, Enterobacter hormaechei, Enterococcus faecium, Klebsiella pneumoniae, Pantoea agglomerans, Salmonella enterica, Serratia marcescens, Staphylococcus sciuri and Weissella confusa. The bacterial compositions of 4 fermented samples were further elucidated using the plate count method. The bacteria isolated from the koji-making stage exhibited the highest diversity; Brachybacterium rhamnosum, E. hormaechei, K. pneumoniae, Kurthia gibsonii, Pantoea dispersa, Staphylococcus gallinarum, Staphylococcus kloosii and S. sciuri were identified. Koji collected during the preincubation stage presented the largest cell counts, and E. hormaechei, K. pneumoniae, E. cloacae and Enterobacter pulveris were identified. In brine samples aged for 7 and 31 days, the majority of the bacteria isolated belonged to 4 Bacillus species, but 4 Staphylococcus species and Delftia tsuruhatensis were also detected. This study demonstrates the benefits of using a combined approach to obtain a more complete picture of microbial populations and provides useful information for the control or development of bacterial flora during inyu fermentation.

  13. Comparison of plate counts, Petrifilm, dipslides, and adenosine triphosphate bioluminescence for monitoring bacteria in cooling-tower waters.

    Science.gov (United States)

    Mueller, Sherry A; Anderson, James E; Kim, Byung R; Ball, James C

    2009-04-01

    Effective bacterial control in cooling-tower systems requires accurate and timely methods to count bacteria. Plate-count methods are difficult to implement on-site, because they are time- and labor-intensive and require sterile techniques. Several field-applicable methods (dipslides, Petrifilm, and adenosine triphosphate [ATP] bioluminescence) were compared with the plate count for two sample matrices--phosphate-buffered saline solution containing a pure culture of Pseudomonas fluorescens and cooling-tower water containing an undefined mixed bacterial culture. For the pure culture, (1) counts determined on nutrient agar and plate-count agar (PCA) media and expressed as colony-forming units (CFU) per milliliter were equivalent to those on R2A medium (p = 1.0 and p = 1.0, respectively); (2) Petrifilm counts were not significantly different from R2A plate counts (p = 0.99); (3) the dipslide counts were up to 2 log units higher than R2A plate counts, but this discrepancy was not statistically significant (p = 0.06); and (4) a discernable correlation (r2 = 0.67) existed between ATP readings and plate counts. For cooling-tower water samples (n = 62), (1) bacterial counts using R2A medium were higher (but not significant; p = 0.63) than nutrient agar and significantly higher than tryptone-glucose yeast extract (TGE; p = 0.03) and PCA (p ATP readings and plate counts varied from system to system, was poor (r2 values ranged from ATP method was not sufficiently sensitive to measure counts below approximately 10(4) CFU/mL.

  14. Evaluation of heterotrophic plate and chromogenic agar colony counting in water quality laboratories.

    Science.gov (United States)

    Hallas, Gary; Monis, Paul

    2015-01-01

    The enumeration of bacteria using plate-based counts is a core technique used by food and water microbiology testing laboratories. However, manual counting of bacterial colonies is both time and labour intensive, can vary between operators and also requires manual entry of results into laboratory information management systems, which can be a source of data entry error. An alternative is to use automated digital colony counters, but there is a lack of peer-reviewed validation data to allow incorporation into standards. We compared the performance of digital counting technology (ProtoCOL3) against manual counting using criteria defined in internationally recognized standard methods. Digital colony counting provided a robust, standardized system suitable for adoption in a commercial testing environment. The digital technology has several advantages:•Improved measurement of uncertainty by using a standard and consistent counting methodology with less operator error.•Efficiency for labour and time (reduced cost).•Elimination of manual entry of data onto LIMS.•Faster result reporting to customers.

  15. Campylobacter inoculation and quantification from broiler cecal samples to compare two plate counting methodologies

    Directory of Open Access Journals (Sweden)

    Anderlise Borsoi

    2015-02-01

    Full Text Available Campylobacteriosis is a zoonosis, a disease transmitted to humans from animals or animal products. The primarily source of Campylobacter infection in human is believed to be the handling and/or consumption of contaminated meat, especially poultry meat. Although in humans such infections are generally self-limiting, complications can arise and may include bacteraemia, Guillain-Barré syndrome, reactive arthritis and abortion. In this study, 32 birds were divided in 2 groups: a control (C group and an inoculated (I group, with 16 birds each. The I group was inoculated orally with 108 CFU/mL of Campylobacter jejuni ATCC 33291, whereas the C group was inoculated with a saline solution. Four chicks per group were euthanized by cervical dislocation at 0, 7, 14 and 21 days post-inoculation (pi. Cecum samples were collected for microbiological analyses. The samples were processed by two plate count methodologies, one developed by the USDA (United States Department of Agriculture in 2011 (B method and the other a serial dilution direct count method (A method. All birds from the C group remained negative until day 21. For the I group, the B method was found to be statistically superior to the A method for counting the recovered cells from the cecal contents at 7, 14 and 21 days pi. The microbiological direct plating counting method is a cost effective and rapid method to determine the level of contamination in broilers to help risk assessment programs at the industry level.

  16. Temperature-independent pectin gel method for aerobic plate count in dairy and nondairy food products: collaborative study.

    Science.gov (United States)

    Roth, J N

    1988-01-01

    Ten laboratories participated in a collaborative study to compare the pectin-based plate count (PC) Redigel method with the aerobic plate count and standard plate count agar-based standard methods for the estimation of total bacterial counts in 9 different nondairy food and dairy food products. The foods were cream, homogenized milk, raw milk, cheese, raw chicken, raw oysters, frozen broccoli, flour, and spices. Each laboratory analyzed 6 samples (3 sample pairs) of each food group. Counts obtained by the pectin-based plate count and agar-based plate count methods differed significantly (P less than 0.05) only for homogenized milk, where the pectin gel method resulted in higher counts. The actual counts were higher in the pectin gel method in 8 of the 9 food groups. The log means for pectin gel and agar-based media, respectively, for the 9 food groups were: cream 8.106 and 7.844; homogenized milk 8.642 and 8.231; raw milk 8.711 and 8.423; chicken 7.654 and 7.645; oysters 7.201 and 7.180; broccoli 7.102 and 6.798; cheese 8.045 and 8.055; flour 4.112 and 3.988; spice 5.379 and 5.314. The repeatability standard deviations favored the pectin gel method in 6 of the 9 foods tested. The reproducibility standard deviations favored the pectin gel method in 7 of the 9 foods tested. These results strongly support the suitability of the pectin gel method as an alternative to agar-based plate count and other methods for total bacterial counts in nondairy and dairy food products. The pectin gel method has been adopted official first action.

  17. Authentic Active Learning Activities Demonstrating the Use of Serial Dilutions and Plate Counts

    Directory of Open Access Journals (Sweden)

    Jordon K. March

    2011-09-01

    Full Text Available Serial dilution and plate counting is often taught in courses for both microbiology and allied health students. Lecture examples and examination questions addressing how the method is used can sometimes be contrived: artificial data sets may have little or no meaning other than to have students perform a calculation. Here we provide a set of activities employing data sets acquired from the primary literature. Our objective was to have the students think critically about a real scenario in which serial dilution and plate count was used. Each activity requires students to read a paragraph describing the study, predict the results, perform the appropriate calculations, and then evaluate the results in light of their predictions. To test the efficacy of these activities, a pretest quiz was given to approximately 100 students in an allied health/general microbiology course. After a lecture on how microbes are enumerated, students were given a different quiz. The class was then divided randomly into groups of three or four students and assigned one of the activities. A postactivity quiz was also administered. Approximately two weeks later, a serial dilution/plate count question was used on an examination and served as a final posttest. Standardized learning gains were calculated for the quiz administered after each learning activity. Even though learning gains were significantly higher after the lecture, there was also a significant improvement between the lecture and the activity. Using an exercise based on an authentic set of data significantly improved student learning gains, and is a useful practice for teaching microbiology.

  18. Enumeration and characterization of standard plate count bacteria in chlorinated and raw water supplies.

    Science.gov (United States)

    LeChevallier, M W; Seidler, R J; Evans, T M

    1980-11-01

    Nearly 700 standard plate count (SPC) bacteria were isolated from drinking water and untreated surface water and identified according to a scheme developed to permit the rapid, simple classification of microorganisms to genus, species, or group. Actinomycetes and Aeromonas species were the two most common groups of SPC bacteria in chlorinated distribution water. Aeromonas spp. and Enterobacter agglomerans were the two most common groups of SPC bacteria in raw water. Identification of bacterial populations before and after contact with chlorine (1 to 2 mg/liter) for 1 h revealed that chlorination selected for gram-positive bacteria. Water that contained high densities of bacteria known to be antagonistic to coliforms had low coliform isolation rates. The membrane filtration technique for enumerating SPC bacteria recovered significantly higher numbers (P standard pour plate technique.

  19. Counting

    Institute of Scientific and Technical Information of China (English)

    许有国

    2005-01-01

    Most people began to count in tens because they had ten fingers on their hands. But in some countries, people counted on one hand and used the three parts of their four fingers. So they counted in twelves, not in tens.

  20. Enumeration of total aerobic microorganisms in foods by SimPlate Total Plate Count-Color Indicator methods and conventional culture methods: collaborative study.

    Science.gov (United States)

    Feldsine, Philip T; Leung, Stephanie C; Lienau, Andrew H; Mui, Linda A; Townsend, David E

    2003-01-01

    The relative efficacy of the SimPlate Total Plate Count-Color Indicator (TPC-CI) method (SimPlate 35 degrees C) was compared with the AOAC Official Method 966.23 (AOAC 35 degrees C) for enumeration of total aerobic microorganisms in foods. The SimPlate TPC-CI method, incubated at 30 degrees C (SimPlate 30 degrees C), was also compared with the International Organization for Standardization (ISO) 4833 method (ISO 30 degrees C). Six food types were analyzed: ground black pepper, flour, nut meats, frozen hamburger patties, frozen fruits, and fresh vegetables. All foods tested were naturally contaminated. Nineteen laboratories throughout North America and Europe participated in the study. Three method comparisons were conducted. In general, there was count difference in recovery among the SimPlate methods and their corresponding reference methods. Mean log counts between the 2 reference methods were also very similar. Repeatability (Sr) and reproducibility (SR) standard deviations were similar among the 3 method comparisons. The SimPlate method (35 degrees C) and the AOAC method were comparable for enumerating total aerobic microorganisms in foods. Similarly, the SimPlate method (30 degrees C) was comparable to the ISO method when samples were prepared and incubated according to the ISO method.

  1. 3M Petrifilm enterobacteriaceae count plate method for enumeration of enterobacteriaceae in selected foods: collaborative study.

    Science.gov (United States)

    Silbernagel, Karen M; Lindberg, Kathryn G

    2003-01-01

    The practice of detecting and enumerating all oxidase-negative, glucose-fermenting-Gram-negative rods (i.e., the family Enterobacteriaceae) is used to indicate unsanitary or inadequate food processing conditions. The objective of this interlaboratory collaborative study was to evaluate and compare the methods described in Standard Methods for the Examination of Dairy Products (SMEDP) and the Compendium of Methods for the Microbiological Examination of Foods (Compendium) with a commercial product, the 3M Petrifilm Enterobacteriaceae Count Plate, for the recovery of Enterobacteriaceae in foods. Six foods--cheddar cheese, milk, flour, frozen prepared meals, frozen broccoli, and nut pieces--were analyzed for Enterobacteriaceae by 12 collaborating laboratories. For each food tested, the collaborators received 8 blind test portions consisting of a control test portion and 3 levels of inoculated test portion, each in duplicate. Each test portion was tested by the Petrifilm Enterobacteriaceae Count Plate method as well as the SMEDP or Compendium methods. The precision estimates (repeatability or within-laboratory variation, and reproducibility or between-laboratory variation) were calculated with standard statistical techniques.

  2. Comparison of Primary Models to Predict Microbial Growth by the Plate Count and Absorbance Methods.

    Science.gov (United States)

    Pla, María-Leonor; Oltra, Sandra; Esteban, María-Dolores; Andreu, Santiago; Palop, Alfredo

    2015-01-01

    The selection of a primary model to describe microbial growth in predictive food microbiology often appears to be subjective. The objective of this research was to check the performance of different mathematical models in predicting growth parameters, both by absorbance and plate count methods. For this purpose, growth curves of three different microorganisms (Bacillus cereus, Listeria monocytogenes, and Escherichia coli) grown under the same conditions, but with different initial concentrations each, were analysed. When measuring the microbial growth of each microorganism by optical density, almost all models provided quite high goodness of fit (r(2) > 0.93) for all growth curves. The growth rate remained approximately constant for all growth curves of each microorganism, when considering one growth model, but differences were found among models. Three-phase linear model provided the lowest variation for growth rate values for all three microorganisms. Baranyi model gave a variation marginally higher, despite a much better overall fitting. When measuring the microbial growth by plate count, similar results were obtained. These results provide insight into predictive microbiology and will help food microbiologists and researchers to choose the proper primary growth predictive model.

  3. Comparative detection of bacterial adhesion to Caco-2 cells with ELISA, radioactivity and plate count methods.

    Science.gov (United States)

    Le Blay, Gwenaëlle; Fliss, Ismaïl; Lacroix, Christophe

    2004-11-01

    Different methods are used to study bacterial adhesion to intestinal epithelial cells, which is an important step in pathogenic infection as well as in probiotic colonization of the intestinal tract. The aim of this study was to compare the ELISA-based method with more conventional plate count and radiolabeling methods for bacterial adhesion detection. An ELISA-based assay was optimized for the detection of Bifidobacterium longum and Escherichia coli O157:H7, which are low and highly adherent bacteria, respectively. In agreement with previous investigations, a percentage of adhesion below 1% was obtained for B. longum with ELISA. However, high nonspecific background and low positive signals were measured due to the use of polyclonal antibodies and the low adhesion capacity with this strain. In contrast, the ELISA-based method developed for E. coli adhesion detected a high adhesion percentage (15%). For this bacterium the three methods tested gave similar results for the highest bacterial concentrations (6.8 Log CFU added bacteria/well). However, differences among methods increased with the addition of decreased bacterial concentration due to different detection thresholds (5.9, 5.6 and 2.9 Log CFU adherent bacteria/well for radioactivity, ELISA and plate count methods, respectively). The ELISA-based method was shown to be a good predictor for bacterial adhesion compared to the radiolabeling method when good quality specific antibodies were used. This technique is convenient and allows handling of numerous samples.

  4. Evaluation of the 3M Petrifilm Enterobacteriaceae Count plate method for the enumeration of Enterobacteriaceae in foods.

    Science.gov (United States)

    Silbernagel, Karen M; Lindberg, Kathryn G

    2002-09-01

    Results of the 3M Petrifilm Enterobacteriaceae Count (EB) plate method were compared with those of the standard violet red bile glucose agar (VRBG) method for the detection and enumeration of Enterobacteriaceae. Studies involving 107 bacterial strains demonstrated that the Petrifilm EB plate method is as sensitive as and more selective than the VRBG method. Sixty of the 62 pure Enterobacteriaceae cultures were recovered by both methods. In addition, 38 of the 45 non-Enterobacteriaceae organisms did not grow on the Petrifilm EB plate, while 28 of the 45 non-Enterobacteriaceae organisms did not grow on the VRBG plate. Colony counts from 174 naturally contaminated and 120 artificially inoculated dairy and nondairy food samples showed that the Petrifilm EB plate method performed as well as or better than the standard VRBG method for the enumeration of Enterobacteriaceae.

  5. Technical note: enumeration of mesophilic aerobes in milk: evaluation of standard official protocols and Petrifilm aerobic count plates.

    Science.gov (United States)

    Freitas, R; Nero, L A; Carvalho, A F

    2009-07-01

    Enumeration of mesophilic aerobes (MA) is the main quality and hygiene parameter for raw and pasteurized milk. High levels of these microorganisms indicate poor conditions in production, storage, and processing of milk, and also the presence of pathogens. Fifteen raw and 15 pasteurized milk samples were submitted for MA enumeration by a conventional plating method (using plate count agar) and Petrifilm Aerobic Count plates (3M, St. Paul, MN), followed by incubation according to 3 official protocols: IDF/ISO (incubation at 30 degrees C for 72 h), American Public Health Association (32 degrees C for 48 h), and Brazilian Ministry of Agriculture (36 degrees C for 48 h). The results were compared by linear regression and ANOVA. Considering the results from conventional methodology, good correlation indices and absence of significant differences between mean counts were observed, independent of type of milk sample (raw or pasteurized) and incubation conditions (IDF/ISO, American Public Health Association, or Ministry of Agriculture). Considering the results from Petrifilm Aerobic Count plates, good correlation indices and absence of significant differences were only observed for raw milk samples. The microbiota of pasteurized milk interfered negatively with the performance of Petrifilm Aerobic Count plates, probably because of the presence of microorganisms that poorly reduce the dye indicator of this system.

  6. Health concerns of heterotrophic plate count (HPC) bacteria in dental equipment water lines.

    Science.gov (United States)

    Allen, Martin J; Edberg, Stephen C

    2016-06-01

    There is an unsubstantiated concern as to the health relevance of HPC (heterotrophic plate count) bacteria in dental equipment waterlines. The American Dental Association (ADA) web site includes guidelines for controlling HPC populations and implies that HPC populations >500 CFU/mL as a "health" benchmark. The world-wide published literature including the United Nations fully examined this situation and concluded that HPC bacteria are not a health risk, but merely a general water quality parameter for all waters including dental water lines. This review provides documentation that the standard measurement of HPC bacteria in waters alone do not pose a health risk and the ADA already provides appropriate practices to minimize HPC bacteria in dental equipment water.

  7. Petrifilm rapid S. aureus Count Plate method for rapid enumeration of Staphylococcus aureus in selected foods: collaborative study.

    Science.gov (United States)

    Silbernagel, K M; Lindberg, K G

    2001-01-01

    A rehydratable dry-film plating method for Staphylococcus aureus in foods, the 3M Petrifilm Rapid S. aureus Count Plate method, was compared with AOAC Official Method 975.55 (Staphylococcus aureus in Foods). Nine foods-instant nonfat dried milk, dry seasoned vegetable coating, frozen hash browns, frozen cooked chicken patty, frozen ground raw pork, shredded cheddar cheese, fresh green beans, pasta filled with beef and cheese, and egg custard-were analyzed for S. aureus by 13 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample and 3 levels of inoculated test sample, each in duplicate. The mean log counts for the methods were comparable for pasta filled with beef and cheese; frozen hash browns; cooked chicken patty; egg custard; frozen ground raw pork; and instant nonfat dried milk. The repeatability and reproducibility variances of the Petrifilm Rapid S. aureus Count Plate method were similar to those of the standard method.

  8. Buckling analysis of a laminate plate

    Directory of Open Access Journals (Sweden)

    Mamuzić, I.

    2008-04-01

    Full Text Available The paper deals with a modeling of laminate plates and with their buckling analysis. To predict the inception of buckling for plates in plane resultant forces must be included. The buckling analysis is made by the help of finite element method in program COSMOS/M. For rectangular laminate plate consisting of 4 layers with symmetric and antisymmetric stacking sequence a buckling analysis is carried out. In the illustrative example there are depicted buckling modes for symmetric laminates [30/-30]s, [45/-45]s, [60/-60]s, [90/-90]s and results of the buckling analysis for the symmetric and antisymmetric laminates.

  9. Comparison of a quantitative microtiter method, a quantitative automated method, and the plate-count method for determining microbial complement resistance.

    Science.gov (United States)

    Lee, M D; Wooley, R E; Brown, J; Spears, K R; Nolan, L K; Shotts, E B

    1991-01-01

    A quantitative microtiter method for determining the degree of complement resistance or sensitivity of microorganisms is described. The microtiter method is compared with a quantitative automated system and the standard plate-count technique. Data were accumulated from 30 avian Escherichia coli isolates incubated at 35 C with either chicken plasma or heat-inactivated chicken plasma. Analysis of data generated by the automated system and plate-count techniques resulted in a classification of the microorganisms into three groups: those sensitive to the action of complement; those of intermediate sensitivity to the action of complement; and those resistant to the action of complement. Although the three methods studied did not agree absolutely, there were statistically significant correlations among them.

  10. Influence of Heat Shock Temperatures and Fast Freezing on Viability of Probiotic Sporeformers and the Issue of Spore Plate Count Versus True Numbers

    Directory of Open Access Journals (Sweden)

    Mojtaba Jafari

    2016-02-01

    Full Text Available Background and Objectives: The purpose of the present study was to investigate effects of various heat shock conditions and fast freezing and subsequent thawing on the viability and recovery of Bacillus coagulans and Bacillus subtilis as probiotic sporeformers, and also to compare spore plate and microscopic counts. Materials and Methods: After preparing the final suspensions of B. coagulans and Bacillus subtilis subsp. Natto spores, they were spread-plated before and after fast freezing treatment (-70°C for about 1 min. Heat shock treatments of the spores were carried out at 68oC for 15, 20, and 30 min as well as at 80oC for 10 and 15 min. Concentrations of the examined probiotic sporeformers were determined simultaneously by plate enumerations and microscopically determined counts. Student’s t-test and one-way analysis of variance (ANOVA of SPSS were used for statistical analysis of the data. Analysis of DoE results was carried out using Minitab. Results: The results presented here show that the highest recovery rates for B. coagulans (14.75 log CFU/mL and B. subtilis spores (14.80 log CFU/mL were under a heat shock condition of 68°C for 20 min in nutrient agar (p<0.05. In addition, the survival rates of B. coagulans and B. subtilis spores under the fast freezing and subsequent thawing condition were about 90% and 88%, respectively. Plate counts differed significantly from counts determined microscopically, with differences of almost 0.5 and 0.8 log for B. coagulans and B. subtilis spores, respectively (p<0.05. In addition, DoE results of the study revealed that both factors of spore count method and only freezing factor in fast freezing treatment have a significant effect on concentrations of the spores examined (p<0.05. Conclusions: Heat shock conditions, freezing and subsequent thawing circumstances, and plate counts or enumerations determined microscopically have significant influences on the viability of probiotic sporeformers and

  11. Dry rehydratable film method for rapid enumeration of coliforms in foods (3M Petrifilm Rapid Coliform Count plate): collaborative study.

    Science.gov (United States)

    Kinneberg, Karen M; Lindberg, Kathryn G

    2002-01-01

    A rehydratable dry-film plating method for coliforms in foods, the 3M Petrifilm Rapid Coliform Count plate method, was compared with the U.S. Food and Drug Administration's Bacteriological Analytical Manual method for nondairy foods and the American Public Health Association's Standard Methods for the Examination of Dairy Products (SMEDP) method for dairy foods. Six food types, vanilla ice cream, cheddar cheese, fresh refrigerated uncooked pasta, wheat flour, prepared frozen macaroni and cheese, and frozen hash browns, were analyzed for coliforms by 11 collaborating laboratories. For each food product tested, the collaborators received 8 blind samples consisting of a control sample and 3 levels of inoculated sample, each in duplicate. The mean log counts for the methods were comparable. The repeatability and reproducibility variances of the Petrifilm Rapid Coliform Count method at 14 and 24 h were not significantly different from those of the standard methods.

  12. 3M Petrifilm Staph Express Count plate method for the enumeration of Staphylococcus aureus in selected dairy foods: collaborative study.

    Science.gov (United States)

    Silbernagel, Karen M; Jechorek, Robert P; Carver, Charles N; Horter, Barbara L; Lindberg, Kathryn G

    2003-01-01

    The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method 975.55 for the enumeration of Staphylococcus aureus in selected foods. Five foods--ice cream, raw milk, yogurt, whey powder, and cheese--were analyzed for S. aureus by 12 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample, a low inoculation level, a medium inoculation level, and a medium inoculation level with background flora, each in duplicate. The mean log10 counts for the methods were comparable for all 5 foods. The repeatability and reproducibility variances of the 24 h Petrifilm Staph Express Count plate method were similar to those of the 72 h standard method.

  13. Do different standard plate counting (IDF/ISSO or AOAC) methods interfere in the conversion of individual bacteria counts to colony forming units in raw milk?

    Science.gov (United States)

    Cassoli, L D; Lima, W J F; Esguerra, J C; Da Silva, J; Machado, P F; Mourão, G B

    2016-10-01

    This study aimed to establish the correlation between individual bacterial count (IBC) obtained by flow cytometry and the number of colony forming units (CFU) determined by standard plate count (SPC) in raw milk using two different reference methodologies: the methodology of the International Dairy Federation (IDF) - International Organization for Standardization (ISO) 4833, incubation for 72 h at 30°C and the methodology of the Association of Official Analytical Chemists (AOAC), incubation for 48 h at 35°C. For this, 100 bovine milk samples (80 ml) from different farms were collected in a sterile bottle and maintained refrigerated at 4°C and were delivered to the laboratory. In the laboratory, the samples were divided into two vials of 40 ml each. Then, half of the vials were forwarded for the SPC analysis, and the other half were analysed using the equipment BactoScan FC. The analyses by flow cytometry and SPC were performed at the same time (maximum deviation of +/- 1 h). To transform the data from IBC ml(-1) to CFU ml(-1) (IDF or AOAC methodology), a standard linear regression equation was used, as recommended by IDF/ISO-196. The difference between the reference methodologies affects the equation that transforms IBC into CFU and therefore the accuracy of the results. The results estimated by the equation using the ISO 4833 methodology were on average 0·18 log units higher than the results estimated using the equation using the AOAC methodology. After the comparison of the methodologies, it was concluded that there is an impact of the reference methodologies on the conversion of the results from IBC to CFU. Depending on the methodology adopted by each laboratory or country, there may not be equivalence in the results. Hence, the laboratories specialized in milk quality analysis that have changed their methodology for analysis, passing from the MAPA (AOAC) methodology to the IDF standard, need to develop new conversion equations to make their

  14. Selection of antibiotic-resistant standard plate count bacteria during water treatment.

    Science.gov (United States)

    Armstrong, J L; Calomiris, J J; Seidler, R J

    1982-08-01

    Standard plate count (SPC) bacteria were isolated from a drinking-water treatment facility and from the river supplying the facility. All isolates were identified and tested for their resistance to six antibiotics to determine if drug-resistant bacteria were selected for as a consequence of water treatment. Among the isolates surviving our test procedures, there was a significant selection (P less than 0.05) of gram-negative SPC organisms resistant to two or more of the test antibiotics. These bacteria were isolated from the flash mix tank, where chlorine, alum, and lime are added to the water. Streptomycin resistance in particular was more frequent in this population as compared with bacteria in the untreated river water (P less than 0.01). SPC bacteria from the clear well, which is a tank holding the finished drinking water at the treatment facility, were also more frequently antibiotic resistant than were the respective river water populations. When 15.8 and 18.2% of the river water bacteria were multiply antibiotic resistant, 57.1 and 43.5%, respectively, of the SPC bacteria in the clear well were multiply antibiotic resistant. Selection for bacteria exhibiting resistance to streptomycin was achieved by chlorinating river water in the laboratory. We concluded that the selective factors operating in the aquatic environment of a water treatment facility can act to increase the proportion of antibiotic-resistant members of the SPC bacterial population in treated drinking water.

  15. Cascaded systems analysis of photon counting detectors

    Energy Technology Data Exchange (ETDEWEB)

    Xu, J.; Zbijewski, W.; Gang, G.; Stayman, J. W. [Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205 (United States); Taguchi, K.; Carrino, J. A. [Russell H. Morgan Department of Radiology, Johns Hopkins University, Baltimore, Maryland 21205 (United States); Lundqvist, M.; Fredenberg, E. [Philips Healthcare, Solna 171 41 (Sweden); Siewerdsen, J. H., E-mail: jeff.siewerdsen@jhu.edu [Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205 (United States); Russell H. Morgan Department of Radiology, Johns Hopkins University, Baltimore, Maryland 21205 (United States)

    2014-10-15

    Purpose: Photon counting detectors (PCDs) are an emerging technology with applications in spectral and low-dose radiographic and tomographic imaging. This paper develops an analytical model of PCD imaging performance, including the system gain, modulation transfer function (MTF), noise-power spectrum (NPS), and detective quantum efficiency (DQE). Methods: A cascaded systems analysis model describing the propagation of quanta through the imaging chain was developed. The model was validated in comparison to the physical performance of a silicon-strip PCD implemented on an experimental imaging bench. The signal response, MTF, and NPS were measured and compared to theory as a function of exposure conditions (70 kVp, 1–7 mA), detector threshold, and readout mode (i.e., the option for coincidence detection). The model sheds new light on the dependence of spatial resolution, charge sharing, and additive noise effects on threshold selection and was used to investigate the factors governing PCD performance, including the fundamental advantages and limitations of PCDs in comparison to energy-integrating detectors (EIDs) in the linear regime for which pulse pileup can be ignored. Results: The detector exhibited highly linear mean signal response across the system operating range and agreed well with theoretical prediction, as did the system MTF and NPS. The DQE analyzed as a function of kilovolt (peak), exposure, detector threshold, and readout mode revealed important considerations for system optimization. The model also demonstrated the important implications of false counts from both additive electronic noise and charge sharing and highlighted the system design and operational parameters that most affect detector performance in the presence of such factors: for example, increasing the detector threshold from 0 to 100 (arbitrary units of pulse height threshold roughly equivalent to 0.5 and 6 keV energy threshold, respectively), increased the f{sub 50} (spatial

  16. Numerical analysis of cross shear plate rolling

    DEFF Research Database (Denmark)

    Zhang, Wenqi; Bay, Niels

    1997-01-01

    The rolling process is widely applied for industrial production of metal plates. In conventional plate rolling the two work rolls are rotating at the same peripheral speed. By introducing a specific difference in the speed of the two work rolls, cross shear rolling is introduced forming a central...... are in the roll gap, the position and the size of the shear zone and the rolling load are calculated. Experimental results are presented verifying the calculations. The numerical analysis facilitates a better understanding of the mechanics in cross shear plate rolling....

  17. Aerobic plate counts and ATP levels correlate with Listeria monocytogenes detection in retail delis.

    Science.gov (United States)

    Hammons, Susan R; Stasiewicz, Matthew J; Roof, Sherry; Oliver, Haley F

    2015-04-01

    Listeria monocytogenes is a foodborne pathogen that causes an estimated 1,591 cases of illness and 255 deaths annually in the United States, the majority of which are attributed to ready-to-eat deli meats processed in retail delis. Because retail delis distribute product directly to consumers, rapid methods to validate cleaning and sanitation are needed to improve retail food safety. This study investigated the relationships among ATP levels, standard aerobic plate count (APC), and L. monocytogenes presence in fully operational delis. Fifteen full-service delis were concurrently sampled for ATP, APC, and L. monocytogenes during preoperational hours once monthly for 3 months. Fifteen additional delis were recruited for 6 months of operational sampling (n = 30). A 1-log increase in APC was equivalent to a 3.3-fold increase in the odds of detecting L. monocytogenes (P < 0.001) and a 1.9-log increase in L monocytogenes population (P = 0.03). An ATP level increase of 1 log relative light unit correlated to a 0.22-log increase in APC (P < 0.001). A preoperational ATP level mean increase by 1 log relative light unit increased the odds of detecting L. monocytogenes concurrently fourfold. A 0.5-log increase in mean ATP level during preoperational sampling corresponded to a 2% increase in the predicted L. monocytogenes prevalence during operation (P < 0.01). Additionally, 10 statistically representative sites were identified and recommended for use in sanitation monitoring programs. Our data support the use of ATP as a rapid method to validate effective cleaning and sanitation to reduce L. monocytogenes in retail delis.

  18. Heterotrophic plate count and consumer's health under special consideration of water softeners.

    Science.gov (United States)

    Hambsch, Beate; Sacré, Clara; Wagner, Ivo

    2004-05-01

    The phenomenon of bacterial growth in water softeners is well known since years. To upgrade the hygienic safety of water softeners, the German DIN Standard 19636 was developed, to assure that the distribution system could not be contaminated by these devices and that the drinking water to be used in the household still meets the microbiological standards according to the German drinking water guidelines, i.e. among others heterotrophic plate count (HPC) below 100 CFU/ml. Moreover, the standard for the water softeners includes a test for contamination with Pseudomonas aeruginosa which has to be disinfected during the regeneration phase. This is possible by sanitizing the resin bed during regeneration by producing chlorine. The results of the last 10 years of tests of water softeners according to DIN 19636 showed that it is possible to produce water softeners that comply with that standard. Approximately 60% of the tested models were accepted. P. aeruginosa is used as an indicator for potentially pathogenic bacteria being able to grow also in low nutrient conditions which normally prevail in drinking water. Like other heterotrophs, the numbers of P. aeruginosa increase rapidly as stagnation occurs. Normally P. aeruginosa is not present in the distributed drinking water. However, under certain conditions, P. aeruginosa can be introduced into the drinking water distribution system, for instance, during construction work. The occurrence of P. aeruginosa is shown in different cases in treatment plants, public drinking water systems and in-house installations. The compliance with DIN 19636 provides assurance that a water softener will not be a constant source of contamination, even if it is once inoculated with a potentially pathogenic bacterium like P. aeruginosa.

  19. Effect of irradiation and storage post-irradiation of black pepper (Piper nigrum L.) on counts of microorganisms hygienic indicator using methods of conventional analysis and PETRIFILM{sup TM} plates; Efeito da irradiacao e do armazenamento pos-irradiacao da pimenta preta (Piper nigrum L.) na contagem de microorganismos indicadores de higiene usando metodos de analise convencionais e placas PETRIFILM{sup TM}

    Energy Technology Data Exchange (ETDEWEB)

    Jaimes, Marcial Ibo Silva

    1988-07-01

    Fifteen samples of ground black pepper (Piper nigrum L.) purchased in Sao Paulo local stores, were submitted to irradiation in doses of 3, 6 and 10 kGy. All irradiated samples, including non-irradiated controls, were submitted to counts of yeasts and molds, aerobes (APC), coliforms and mesophilic aerobic spore formers (MASC), using conventional plate count methods and PETRIFILM {sup TM} plates. For yeasts and molds count, acidified potato dextrose agar (PDA) an PETRIFILM {sup TM} PFYM plates were used. For aerobes, plate count agar (PCA) and PETRIFILM {sup TM} PFAC plates were used. Violet red bile agar (VRBA) and PETRIFILM {sup TM} PFEC plates were employed for enumeration of coliforms. Counts of these groups of microorganisms obtained through the traditional plating procedures did not differ significantly from those using the corresponding PETRIFILM {sup TM} plates. In samples submitted to irradiation, a dose of 10 kGy caused a decrease of the yeasts and molds count from 10{sup 4}-10{sup 5} to less than 10 cfu/g. The same dose caused a decrease of the aerobic counts from 10{sup 7}-10{sup 8} to 10{sup 2}-10{sup 3} cfu/g, of coliforms from 10{sup 4}-10{sup 5} to less than 10 cfu/g and MASC from 10{sup 6}-10{sup 7} cfu/g to 10-10{sup 2} cfu/g. The introduction of a injury repair step in the counting procedure resulted in a 32 to 89% increase in the number of coliforms. However, this additional step did not improve significantly the counts of MASC. After 270 days of storage of samples irradiated with 3 kGy, a decrease in the yeasts and molds population from 10{sup 3} to 20 cfu/g was observed. The APC population in these samples was reduced from 5,0x10{sup 6} to 2,4x10{sup 4} cfu/g; in those irradiated with 6 kGy the reduction was from 4,0x10{sup 4} to 5,0x10{sup 3} cfu/g and in those irradiated with 10 kGy the counts were reduced from 30 to less than 10 cfu/g. After the same time of storage, the coliform population in non irradiated samples decreased from 2,8x10{sup

  20. Analysis of General Power Counting Rules in Effective Field Theory

    CERN Document Server

    Gavela, B M; Manohar, A V; Merlo, L

    2016-01-01

    We derive the general counting rules for a quantum effective field theory (EFT) in $\\mathsf{d}$ dimensions. The rules are valid for strongly and weakly coupled theories, and predict that all kinetic energy terms are canonically normalized. They determine the energy dependence of scattering cross sections in the range of validity of the EFT expansion. The size of cross sections is controlled by the $\\Lambda$ power counting of EFT, not by chiral counting, even for chiral perturbation theory ($\\chi$PT). The relation between $\\Lambda$ and $f$ is generalized to $\\mathsf{d}$ dimensions. We show that the naive dimensional analysis $4\\pi$ counting is related to $\\hbar$ counting. The EFT counting rules are applied to $\\chi$PT, to Standard Model EFT and to the non-trivial case of Higgs EFT, which combines the $\\Lambda$ and chiral counting rules within a single theory.

  1. A multilevel analysis of intercompany claim counts

    NARCIS (Netherlands)

    Antonio, K.; Frees, E.W.; Valdez, E.A.

    2009-01-01

    In this paper, we use multilevel models to analyze data on claim counts provided by the General Insurance Association of Singapore, an organization consisting of most of the general insurers in Singapore. Our data comes from the financial records of automobile insurance policies followed over a peri

  2. A multilevel analysis of intercompany claim counts

    NARCIS (Netherlands)

    Antonio, K.; Frees, E.W.; Valdez, E.A.

    2009-01-01

    In this paper, we use multilevel models to analyze data on claim counts provided by the General Insurance Association of Singapore, an organization consisting of most of the general insurers in Singapore. Our data comes from the financial records of automobile insurance policies followed over a peri

  3. Comparison of a rapid ATP bioluminescence assay and standard plate count methods for assessing microbial contamination of consumers' refrigerators.

    Science.gov (United States)

    Chen, Fur-Chi; Godwin, Sandria L

    2006-10-01

    The feasibility of using an ATP bioluminescence assay for assessing microbial contamination of home refrigerators was evaluated and compared with the standard culture methods. Samples of refrigerator surfaces were collected from 123 households by swabbing an area of 100 cm2 on three locations in the refrigerator with premoisturized sterile swabs. Microbial contaminations were determined by aerobic plate count (APC; incubated at 35 degrees C for 48 h) and psychrotrophic plate count (PPC; incubated at 7 degrees C for 10 days) on plate count agar. The results were compared to the readings from the microbial ATP (mATP) bioluminescence assay. The correlation coefficient (r) between mATP and PPC (r = 0.851) was slightly higher than that between mATP and APC (r = 0.823). Our results indicated a potential discrepancy in the population of mesophilic and psychrotrophic bacteria in the refrigerator samples. Nevertheless, mATP appeared to be a reliable indication of the average of APC and PPC (r = 0.895). The mATP bioluminescence assay would provide a rapid and convenient test for researchers in field studies to assess microbial contamination in refrigerators.

  4. Real-time ArcGIS and heterotrophic plate count based chloramine disinfectant control in water distribution system.

    Science.gov (United States)

    Bai, Xiaohui; Zhi, Xinghua; Zhu, Huifeng; Meng, Mingqun; Zhang, Mingde

    2015-01-01

    This study investigates the effect of chloramine residual on bacteria growth and regrowth and the relationship between heterotrophic plate counts (HPCs) and the concentration of chloramine residual in the Shanghai drinking water distribution system (DWDS). In this study, models to control HPCs in the water distribution system and consumer taps are also developed. Real-time ArcGIS was applied to show the distribution and changed results of the chloramine residual concentration in the pipe system by using these models. Residual regression analysis was used to get a reasonable range of the threshold values that allows the chloramine residual to efficiently inhibit bacteria growth in the Shanghai DWDS; the threshold values should be between 0.45 and 0.5 mg/L in pipe water and 0.2 and 0.25 mg/L in tap water. The low residual chloramine value (0.05 mg/L) of the Chinese drinking water quality standard may pose a potential health risk for microorganisms that should be improved. Disinfection by-products (DBPs) were detected, but no health risk was identified.

  5. High gain micro-channel plate multipliers for particle tracking or single photo-electron counting

    Energy Technology Data Exchange (ETDEWEB)

    Oba, K.; Rehak, P.; Smith, S.D.

    1980-01-01

    Micro-channel plate multipliers have been used to detect the passage of relativistic charged particles. Measurements of the detection efficiency and pulse height response versus micro-channel plate gain are presented for one, two and three micro-channel plate arrays. Values describing temporal response and transit time jitter are also given. In a separate measurement, the response of a single micro-channel plate to individual slow electrons (less than or equal to 1 keV) was studied. By using a special technique output bunches as small as three electrons may be seen for low average micro-channel plate gain. The results of attempts to improve the micro-channel plate response are also presented.

  6. Analysis of general power counting rules in effective field theory

    Energy Technology Data Exchange (ETDEWEB)

    Gavela, Belen; Merlo, Luca [Universidad Autonoma de Madrid, Instituto de Fisica Teorica, IFT-UAM/CSIC, Madrid (Spain); Jenkins, Elizabeth E.; Manohar, Aneesh V. [University of California at San Diego, Department of Physics, La Jolla, CA (United States); CERN TH Division, Geneva 23 (Switzerland)

    2016-09-15

    We derive the general counting rules for a quantum effective field theory (EFT) in d dimensions. The rules are valid for strongly and weakly coupled theories, and they predict that all kinetic energy terms are canonically normalized. They determine the energy dependence of scattering cross sections in the range of validity of the EFT expansion. We show that the size of the cross sections is controlled by the Λ power counting of EFT, not by chiral counting, even for chiral perturbation theory (χPT). The relation between Λ and f is generalized to d dimensions. We show that the naive dimensional analysiscounting is related to ℎ counting. The EFT counting rules are applied to χPT, low-energy weak interactions, Standard Model EFT and the non-trivial case of Higgs EFT. (orig.)

  7. geoCount: An R Package for the Analysis of Geostatistical Count Data

    Directory of Open Access Journals (Sweden)

    Liang Jing

    2015-02-01

    Full Text Available We describe the R package geoCount for the analysis of geostatistical count data. The package performs Bayesian analysis for the Poisson-lognormal and binomial-logitnormal spatial models, which are subclasses of the class of generalized linear spatial models proposed by Diggle, Tawn, and Moyeed (1998. The package implements the computational intensive tasks in C++ using an R/C++ interface, and has parallel computation capabilities to speed up the computations. geoCount also implements group updating, Langevin- Hastings algorithms and a data-based parameterization, algorithmic approaches proposed by Christensen, Roberts, and Sko ?ld (2006 to improve the efficiency of the Markov chain Monte Carlo algorithms. In addition, the package includes functions for simulation and visualization, as well as three geostatistical count datasets taken from the literature. One of those is used to illustrate the package capabilities. Finally, we provide a side-by-side comparison between geoCount and the R packages geoRglm and INLA.

  8. Comparison and analysis of the aerobic plate count, coliforms, Escherichia coli ,enterobacteriaceae in national and international food safety standards%食品安全微生物学指示菌国内外标准应用的比较分析

    Institute of Scientific and Technical Information of China (English)

    徐进; 庞璐

    2011-01-01

    菌落总数、大肠菌群、大肠埃希菌、肠杆菌科作为食品安全微生物限量的指示菌在国内外标准中的应用不尽相同.本文通过比较我国与欧盟、澳大利亚、新西兰、加拿大和香港地区的相关食品指示菌标准,为制定我国的食品安全微生物标准提供技术依据.%As indicator organisms, standards for aerobic plate count, coliforms, Escherichia coli , enterobacteriaceae applied in each country were different. Based on the comparison and analysis of microbiological criteria of foods in Australia, New Zealand, European Communities, Canada and Hong Kong, recommendations and technical basis for the elaboration of general microbiological criteria of China' s national food safety standards were provided.

  9. Evaluation of the 3M™ Petrifilm™ Rapid Yeast and Mold Count Plate for the Enumeration of Yeast and Mold in Food: Collaborative Study, First Action 2014.05.

    Science.gov (United States)

    Bird, Patrick; Flannery, Jonathan; Crowley, Erin; Agin, James; Goins, David; Jechorek, Robert

    2015-01-01

    The 3M™ Petrifilm™ Rapid Yeast and Mold (RYM) Count Plate is a simple, ready-to-use chromogenic culture method for the rapid detection and enumeration of yeast and mold in food products. The 3M Petrifilm RYM Count Plate method was compared to the U. S. Food and Drug Administration Bacteriological Analytical Manual (FDA BAM) Chapter 18, Yeasts, Molds and Mycotoxins and the ISO 21527:2008 Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Enumeration for Yeast and Molds - Part 1: Colony Count Technique in Products with Water Activity Greater Than 0.95 and Part 2: Colony Count Technique in Products with Water Activity Less Than or Equal to 0.95 reference methods for raw almonds and raw frozen ground beef patties (77% lean). The 3M Petrifilm RYM Count Plate method was evaluated using a paired study design in a multi-laboratory collaborative study following the current AOAC Validation Guidelines. Three target contamination levels (low, 10-100 CFU/g; medium, 100-1000 CFU/g; high 1000-10 000 CFU/g) as well as an uninoculated control level (0 CFU/g) were evaluated for each matrix. Samples evaluated by the 3M Petrifilm RYM Count Plate method were prepared in duplicate and incubated at both 25°C and 28°C. Plates at both temperatures were enumerated after 48 and 60 h of incubation. No significant difference was observed between the 3M Petrifilm RYM Count Plate method and the FDA BAM or ISO 21527 reference methods for each contamination level. No statistical differences were observed between samples analyzed by the 3M Petrifilm RYM Count Plate method (at either 25°C or 28°C) and the reference methods. No statistical significant differences were observed between enumeration of colonies at 48 and 60 h on the 3M Petrifilm RYM Count Plate method and the reference methods.

  10. Applied categorical and count data analysis

    CERN Document Server

    Tang, Wan; Tu, Xin M

    2012-01-01

    Introduction Discrete Outcomes Data Source Outline of the BookReview of Key Statistical ResultsSoftwareContingency Tables Inference for One-Way Frequency TableInference for 2 x 2 TableInference for 2 x r TablesInference for s x r TableMeasures of AssociationSets of Contingency Tables Confounding Effects Sets of 2 x 2 TablesSets of s x r TablesRegression Models for Categorical Response Logistic Regression for Binary ResponseInference about Model ParametersGoodness of FitGeneralized Linear ModelsRegression Models for Polytomous ResponseRegression Models for Count Response Poisson Regression Mode

  11. Standard plate counts of drinking water: a comparison between incubation temperatures of 20 and 30 degrees C.

    Science.gov (United States)

    Spinedi, C; Gisin, M

    1990-08-01

    Standard plate counts of 5085 drinking water samples gathered in the Region of Basle were carried out over a period of 9 years (1977 to 1985). Two conditions of incubation were evaluated: 20 degrees C and 30 degrees C for 72 h. In ground water samples (3048 samples) colony forming units (cfu) at 30 degrees C were found to be higher than counts at 20 degrees C incubation, 45% of the samples contained greater than or equal to 2 cfu/ml at 30 degrees versus 35% at 20 degrees C. The median was 1 cfu/ml at both temperatures. In spring water samples (2036 samples) bacterial counts at 20 degrees C were found to be higher than counts at 30 degrees C incubation, 61% of the samples contained greater than 10 cfu/ml at 20 degrees C versus 51% at 30 degrees C. The median was 19 cfu/ml at 20 degrees C incubation versus 11 cfu/ml at 30 degrees C. These differences were statistically significant with p less than 0.001 (Wilcoxon matched-pairs signed-rank test). No correlation was found between bacterial counts at 20 degrees C and bacterial counts at 30 degrees C, nor between bacterial counts and original water temperatures. It appears that incubation temperatures of 20 degrees C and 30 degrees C favor the growth of different populations of bacteria and temperature is not the only factor. However, from a practical point of view the use of only one incubation temperature seems to be justified for the purpose of judging the sanitary quality of drinking water.

  12. Comparison of Petrifilm Staph Express Count System with the bacteriological analytical manual direct-plating method for enumeration of Staphylococcus aureus in artificially contaminated hard cheese.

    Science.gov (United States)

    Fedio, Willis M; Wendakoon, Chitra N; Zapata, Ruben; Carrillo, Christina; Browning, Paul

    2008-01-01

    The 3M Petrifilm Staph Express Count System was compared with the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) direct-plate count method for the enumeration of Staphylococcus aureus in 6 types of artificially contaminated hard cheese (Asiago, Cheddar, Gruyère, Parmesan, Romano, and Swiss). Five different samples of each cheese type were inoculated with S. aureus (ATCC 25923) to achieve low, medium, and high inoculum levels. S. aureus was enumerated by the Petrifilm and BAM methods, and the results were compared. Multivariate analysis of variance revealed no significant differences (P<0.05) between the 2 methods. The Petrifilm method compared favorably with the BAM procedure. The rapid method was more convenient to use, considerably faster, and less expensive to perform than the BAM method.

  13. Analysis of Biaxially Stressed Bridge Deck Plates

    DEFF Research Database (Denmark)

    Jönsson, Jeppe; Bondum, Tommi Højer

    2012-01-01

    The ultimate state analysis of bridge deck plates at the intersection zone between main girders and transverse beams is complicated by biaxial membrane stresses, which may be in compression or tension in either direction depending on the bridge configuration and the specific location. This paper...... presents a detailed investigation of the ultimate capacity of simply supported plates subjected to biaxial loads. The full interaction domain of combinations of compressive and tensile loads has been investigated including a large number of imperfections. It was found that non-standard imperfection modes...

  14. Numerical analysis of cross shear plate rolling

    DEFF Research Database (Denmark)

    Zhang, Wenqi; Bay, Niels

    1997-01-01

    The rolling process is widely applied for industrial production of metal plates. In conventional plate rolling the two work rolls are rotating at the same peripheral speed. By introducing a specific difference in the speed of the two work rolls, cross shear rolling is introduced forming a central...... shear zone between the forward and backward slip zones in the deformation zone thus lowering the rolling load. A numerical analysis of the cross shear rolling process is carried out based on the slab method adopting Wanheim and Bay's general friction model. The pressure distribution along the contact...

  15. Microfluidic Cytometer for Complete Blood Count Analysis Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We will fabricate and test microfluidic designs for a micro-electromechanical system based complete blood count (CBC) analysis in separate modules and integrate them...

  16. Reliability assessment of different plate theories for elastic wave propagation analysis in functionally graded plates.

    Science.gov (United States)

    Mehrkash, Milad; Azhari, Mojtaba; Mirdamadi, Hamid Reza

    2014-01-01

    The importance of elastic wave propagation problem in plates arises from the application of ultrasonic elastic waves in non-destructive evaluation of plate-like structures. However, precise study and analysis of acoustic guided waves especially in non-homogeneous waveguides such as functionally graded plates are so complicated that exact elastodynamic methods are rarely employed in practical applications. Thus, the simple approximate plate theories have attracted much interest for the calculation of wave fields in FGM plates. Therefore, in the current research, the classical plate theory (CPT), first-order shear deformation theory (FSDT) and third-order shear deformation theory (TSDT) are used to obtain the transient responses of flexural waves in FGM plates subjected to transverse impulsive loadings. Moreover, comparing the results with those based on a well recognized hybrid numerical method (HNM), we examine the accuracy of the plate theories for several plates of various thicknesses under excitations of different frequencies. The material properties of the plate are assumed to vary across the plate thickness according to a simple power-law distribution in terms of volume fractions of constituents. In all analyses, spatial Fourier transform together with modal analysis are applied to compute displacement responses of the plates. A comparison of the results demonstrates the reliability ranges of the approximate plate theories for elastic wave propagation analysis in FGM plates. Furthermore, based on various examples, it is shown that whenever the plate theories are used within the appropriate ranges of plate thickness and frequency content, solution process in wave number-time domain based on modal analysis approach is not only sufficient but also efficient for finding the transient waveforms in FGM plates.

  17. Improvements in Boron Plate Coating Technology for Higher Efficiency Neutron Detection and Coincidence Counting Error Reduction

    Energy Technology Data Exchange (ETDEWEB)

    Menlove, Howard Olsen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Henzlova, Daniela [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-08-25

    This informal report presents the measurement data and information to document the performance of the advanced Precision Data Technology, Inc. (PDT) sealed cell boron-10 plate neutron detector that makes use of the advanced coating materials and procedures. In 2015, PDT changed the boron coating materials and application procedures to significantly increase the efficiency of their basic corrugated plate detector performance. A prototype sealed cell unit was supplied to LANL for testing and comparison with prior detector cells. Also, LANL had reference detector slabs from the original neutron collar (UNCL) and the new Antech UNCL with the removable 3He tubes. The comparison data is presented in this report.

  18. PENGARUH PERENDAMAN EKSTRAK BAWANG PUTIH (Allium sativum TERHADAP TOTAL PLATE COUNT DAN SIFAT ORGANOLEPTIK BAKSO DAGING SAPI

    Directory of Open Access Journals (Sweden)

    Yunita Pustikayanti

    2012-10-01

    Full Text Available Tujuan penelitian untuk mengetahui alternatif pengganti boraks pada bahan pangan yang dapat diaplikasikan produsen bakso. Penelitian eksperimen dengan populasi 4 bakso berbagai perlakuan dan 10 panelis menggunakan instrumen alat uji mikrobiologi dan formulir penilaian sifat organoleptik. Analisis data dilakukan secara univariat dan bivariat (Repeated Anova. Hasil penelitian menunjukkan bakso paling disukai adalah bakso tambahan karagenan yang direndam ekstrak bawang putih 30%. Nilai Total Plate Count bakso jam ke-24 yang dibawah 1×105 adalah bakso menggunakan tambahan karagenan dan bakso menggunakan boraks. Ada pengaruh perendaman ekstrak bawang putih terhadap tekstur (0,013, warna (0,022, aroma (0,001. Saran bagi pemerintah hendaknya mensosialisasikan penggunaan bahan tambahan pangan yang lebih aman. BPOM bekerjasama dengan instansi lainnya menyelenggarakan program produk pangan bebas boraks di Indonesia dan mengharuskan produsen bakso mengirimkan sampel bakso ke Laboratorium pengujian makanan untuk diuji keamanan pangannya. Bagi peneliti selanjutnya disarankan meneliti perbedaan nilai Total Plate Count dan sifat organoleptik bakso pada suhu ruang, suhu dingin, dan suhu beku. Serta menambahkan perlakuan bakso dengan penambahan Sodium Tri Poly Phospat (STPP

  19. Development of Microelectrode Arrays Using Electroless Plating for CMOS-Based Direct Counting of Bacterial and HeLa Cells.

    Science.gov (United States)

    Niitsu, Kiichi; Ota, Shoko; Gamo, Kohei; Kondo, Hiroki; Hori, Masaru; Nakazato, Kazuo

    2015-10-01

    The development of two new types of high-density, electroless plated microelectrode arrays for CMOS-based high-sensitivity direct bacteria and HeLa cell counting are presented. For emerging high-sensitivity direct pathogen counting, two technical challenges must be addressed. One is the formation of a bacteria-sized microelectrode, and the other is the development of a high-sensitivity and high-speed amperometry circuit. The requirement for microelectrode formation is that the gold microelectrodes are required to be as small as the target cell. By improving a self-aligned electroless plating technique, the dimensions of the microelectrodes on a CMOS sensor chip in this work were successfully reduced to 1.2 μm × 2.05 μm. This is 1/20th of the smallest size reported in the literature. Since a bacteria-sized microelectrode has a severe limitation on the current flow, the amperometry circuit has to have a high sensitivity and high speed with low noise. In this work, a current buffer was inserted to mitigate the potential fluctuation. Three test chips were fabricated using a 0.6- μm CMOS process: two with 1.2 μm × 2.05 μm (1024 × 1024 and 4 × 4) sensor arrays and one with 6- μm square (16 × 16) sensor arrays; and the microelectrodes were formed on them using electroless plating. The uniformity among the 1024 × 1024 electrodes arranged with a pitch of 3.6 μm × 4.45 μm was optically verified. For improving sensitivity, the trenches on each microelectrode were developed and verified optically and electrochemically for the first time. Higher sensitivity can be achieved by introducing a trench structure than by using a conventional microelectrode formed by contact photolithography. Cyclic voltammetry (CV) measurements obtained using the 1.2 μm × 2.05 μm 4 × 4 and 6- μm square 16 × 16 sensor array with electroless-plated microelectrodes successfully demonstrated direct counting of the bacteria-sized microbeads and HeLa cells.

  20. New Approaches on Quantification of Campylobacter jejuni in Poultry Samples: The Use of Digital PCR and Real-time PCR against the ISO Standard Plate Count Method

    Science.gov (United States)

    Papić, Bojan; Pate, Mateja; Henigman, Urška; Zajc, Urška; Gruntar, Igor; Biasizzo, Majda; Ocepek, Matjaž; Kušar, Darja

    2017-01-01

    Campylobacteriosis is the most frequently reported bacterial food-borne illness in the European Union and contaminated broiler meat is considered the most important source of infection in humans. The aim of the present study was to evaluate real-time PCR (qPCR) and digital PCR (dPCR) for quantification of Campylobacter jejuni in 75 broiler neck-skin samples collected from a poultry slaughterhouse, and to compare them with the ISO 10272-2 standard plate count method. For qPCR standard curve, C. jejuni-negative neck-skin samples were spiked with C. jejuni suspension with a known number of bacterial cells. The observed CFU/g values by qPCR correlated greatly with the expected values and qPCR showed good performance with the reliable limit of detection (rLOD) and limit of quantification (LOQ) of three and 31 target copies per reaction, respectively. However, both rLOD (1219 CFU/g) and LOQ (12,523 CFU/g) were beyond the EFSA-proposed critical limit of 500–1,000 CFU/g of neck skin. Although C. jejuni cell counts were ≤1,000 CFU/g in only 7/75 samples by plate counting, they were ≤LOQ in 60/75 and ≤rLOD in 26/75 (≤1,000 CFU/g in 24/75) samples by qPCR. A strong and statistically significant correlation was observed between qPCR and dPCR. Both PCR-based methods correlated significantly with the plate count method; however, the correlation was moderate. Using the Bland–Altman analysis, an average agreement was noted between all three methods, although with a large standard deviation. A significant bias toward overestimation in dPCR was observed, probably due to the relatively high number of false positive calls. The linear dynamic range was comparable in both PCR-based methods; however, qPCR proved to be more suitable for routine use. In the future, the establishment of a reliable molecular quantification of C. jejuni in poultry samples showing a wide range of contamination levels down to the proposed critical limit is needed to enable time- and cost

  1. Structural Analysis of Plate Based Tensegrity Structures

    DEFF Research Database (Denmark)

    Hald, Frederik; Kirkegaard, Poul Henning; Damkilde, Lars

    2013-01-01

    Plate tensegrity structures combine tension cables with a cross laminated timber plate and can then form e.g. a roof structure. The topology of plate tensegrity structures is investigated through a parametric investigation. Plate tensegrity structures are investigated, and a method...... for determination of the structures pre-stresses is used. A parametric investigation is performed to determine a more optimized form of the plate based tensegrity structure. Conclusions of the use of plate based tensegrity in civil engineering and further research areas are discussed....

  2. Structural Analysis of Plate Based Tensegrity Structures

    DEFF Research Database (Denmark)

    Hald, Frederik; Kirkegaard, Poul Henning; Damkilde, Lars

    2013-01-01

    Plate tensegrity structures combine tension cables with a cross laminated timber plate and can then form e.g. a roof structure. The topology of plate tensegrity structures is investigated through a parametric investigation. Plate tensegrity structures are investigated, and a method...... for determination of the structures pre-stresses is used. A parametric investigation is performed to determine a more optimized form of the plate based tensegrity structure. Conclusions of the use of plate based tensegrity in civil engineering and further research areas are discussed....

  3. Evaluation of the petrifilm aerobic count plate for enumeration of aerobic marine bacteria from seawater and Caulerpa lentillifera.

    Science.gov (United States)

    Kudaka, Jun; Horii, Toru; Tamanaha, Koji; Itokazu, Kiyomasa; Nakamura, Masaji; Taira, Katsuya; Nidaira, Minoru; Okano, Sho; Kitahara, Akio

    2010-08-01

    The enumeration and evaluation of the activity of marine bacteria are important in the food industry. However, detection of marine bacteria in seawater or seafood has not been easy. The Petrifilm aerobic count plate (ACP) is a ready-to-use alternative to the traditional enumeration media used for bacteria associated with food. The purpose of this study was to evaluate the usefulness of a simple detection and enumeration method utilizing the Petrifilm ACP for enumeration of aerobic marine bacteria from seawater and an edible seaweed, Caulerpa lentillifera. The efficiency of enumeration of total aerobic marine bacteria on Petrifilm ACP was compared with that using the spread plate method on marine agar with 80 seawater and 64 C. lentillifera samples. With sterile seawater as the diluent, a close correlation was observed between the method utilizing Petrifilm ACP and that utilizing the conventional marine agar (r=0.98 for seawater and 0.91 for C. lentillifera). The Petrifilm ACP method was simpler and less time-consuming than the conventional method. These results indicate that Petrifilm ACP is a suitable alternative to conventional marine agar for enumeration of marine microorganisms in seawater and C. lentillifera samples.

  4. Development of a single-photon-counting camera with use of a triple-stacked micro-channel plate.

    Science.gov (United States)

    Yasuda, Naruomi; Suzuki, Hitoshi; Katafuchi, Tetsuro

    2016-01-01

    At the quantum-mechanical level, all substances (not merely electromagnetic waves such as light and X-rays) exhibit wave–particle duality. Whereas students of radiation science can easily understand the wave nature of electromagnetic waves, the particle (photon) nature may elude them. Therefore, to assist students in understanding the wave–particle duality of electromagnetic waves, we have developed a photon-counting camera that captures single photons in two-dimensional images. As an image intensifier, this camera has a triple-stacked micro-channel plate (MCP) with an amplification factor of 10(6). The ultra-low light of a single photon entering the camera is first converted to an electron through the photoelectric effect on the photocathode. The electron is intensified by the triple-stacked MCP and then converted to a visible light distribution, which is measured by a high-sensitivity complementary metal oxide semiconductor image sensor. Because it detects individual photons, the photon-counting camera is expected to provide students with a complete understanding of the particle nature of electromagnetic waves. Moreover, it measures ultra-weak light that cannot be detected by ordinary low-sensitivity cameras. Therefore, it is suitable for experimental research on scintillator luminescence, biophoton detection, and similar topics.

  5. Application of microbiological method direct epifluorescence filter techique/aerobic plate count agar in the identification of irradiated herbs and spices

    OpenAIRE

    Maria Concetta Campagna; Maria Teresa Di Schiavi; Marina Foti; Maria Cristina Mosconi; Giuseppina Mattiolo; Roberta Cavallina

    2014-01-01

    Irradiation is a preservation technology used to improve the safety and hygienic quality of food. Aim of this study was to assess the applicability and validity of the microbiological screening method direct epifluorescence filter technique (DEFT)/aerobic plate count (APC) (EN 13783:2001) for the identification of irradiated herbs and spices. Tests on non-irradiated and irradiated samples of dried herbs and spices were performed. The method was based on the comparison of APC and count obtaine...

  6. An Analysis of Elasto-Plastic Bending of Rectangular Plate

    OpenAIRE

    Matsuda, Hiroshi; Sakiyama, Takeshi

    1988-01-01

    In this paper, a discrete method for analyzing the problem of elasto-plastic bending of a rectangular plate is proposed. The solutions for partial differential equation of rectangular plate are obtained in discrete forms by applying numerical integnltion. An incremental variable elasticity procedure has been used for the clasta-plastic analysis of the rectangular plate. As the applications of the proposed method, clasta-plastic bending of rectangular plate with four types of boundary conditio...

  7. Finite element analysis of elasto-plastic plate bending problems using transition rectangular plate elements

    Institute of Scientific and Technical Information of China (English)

    Bahattin Kanber; O.Yavuz Bozkurt

    2006-01-01

    In this work,the finite element analysis of the elasto-plastic plate bending problems is carried out using transition rectangular plate elements.The shape functions of the transition plate elements are derived based on a practical rule.The transition plate elements are all quadrilateral and can be used to obtain efficient finite element models using minimum number of elements.The mesh convergence rates of the models including the transition elements are compared with the regular element models.To verify the developed elements,simple tests are demonstrated and various elasto-plastic problems are solved.Their results are compared with ANSYS results.

  8. Assessment of the efficiency of SimPlate™ total plate count color indicator (TPC CI to quantify mesophilic aerobic microorganisms in pasteurized milk Avaliação da eficiência do SimPlate™ Total Plate Count Color Indicator (TPC CI para enumeração de microrganismos aeróbios mesófilos em leite pasteurizado

    Directory of Open Access Journals (Sweden)

    Luís Augusto Nero

    2002-01-01

    Full Text Available The SimPlate™ TPC CI system is a rapid method to count mesophilic aerobic microorganisms (MAM in foods, based on the use of resazurine to indicate bacterial growth. Its efficiency in pasteurized milk was evaluated using 142 pasteurized milk samples (38 type A, 43 type B and 61 type C collected in Londrina, PR. The standard plating method, using Plate Count Agar (PCA was used for comparison. The plates of both systems were incubated at 35ºC and read after 24h and 48h. The occurrence of false-positive and false-negative wells and the predominant microorganisms in them were also evaluated. The results were compared by simple correlation and mean variance analyses. The correlation (r and mean variance values were 0.6811 and 0.7583 for the results obtained after 24h, respectively, and 0.9126 and 0.0842 for the results obtained after 48h, respectively. These results indicate that the performance of the system increases when the plates are incubated for 48h. When the three types of milk were evaluated separately, these values were 0.9285 and 0.0817 for type A milk, 0.9231 and 0.0466 for type B milk and 0.7209 and 0.1082 for type C milk. These results indicate that the better the quality of the milk the better the performance of SimPlate™ TPC CI. False-negative wells, found more frequently in samples with high MAM counts, were caused by Gram positive microorganisms, poorly detected by the SimPlate™ TPC CI system because they grew slowly and had low reduction capacity. The results indicated a higher efficiency of the SimPlate™ TPC CI system in the reading at 48h.O sistema SimPlate™ TPC CI é um método rápido para enumeração de microrganismos aeróbios mesófilos (MAM em alimentos que utiliza a resazurina como substância indicadora de crescimento bacteriano. Para avaliar sua eficiência em leite pasteurizado, 142 amostras (38 de leite tipo A, 43 de leite tipo B e 61 de leite tipo C foram colhidas em Londrina, PR, e analisadas pelo SimPlate

  9. Evaluation of the 3M™ Petrifilm™ Rapid Aerobic Count Plate for the Enumeration of Aerobic Bacteria: Collaborative Study, First Action 2015.13.

    Science.gov (United States)

    Bird, Patrick; Flannery, Jonathan; Crowley, Erin; Agin, James; Goins, David; Jechorek, Robert

    2016-05-01

    The 3M™ Petrifilm™ Rapid Aerobic Count (RAC) Plate is a sample-ready culture medium system containing dual-sensor indicator technology for the rapid quantification of aerobic bacteria in food products. The 3M Petrifilm RAC Plate was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual (FDA BAM) Chapter 3 (Aerobic Plate Count) for the enumeration of aerobic bacteria in raw easy-peel shrimp and the Standard Methods for the Examination of Dairy Products (SMEDP) Chapter 6 (Standard Plate Count Method) for the enumeration of aerobic bacteria in pasteurized skim milk and instant nonfat dry milk (instant NFDM). The 3M Petrifilm RAC Plate was evaluated using a paired study design in a multilaboratory collaborative study following current AOAC validation guidelines. Three target contamination levels (low, 10-100 CFU/g; medium, 100-1000 CFU/g; and high 1000-10 000 CFU/g) were evaluated for naturally occurring aerobic microflora for each matrix. For raw easy-peel shrimp, duplicate 3M Petrifilm RAC Plates were enumerated after 24 ± 2 h incubation at both 32 and 35°C. Pasteurized skim milk 3M Petrifilm RAC Plates were enumerated after 24 ± 2 h incubation at 32°C, and instant NFDM 3M Petrifilm RAC Plates were enumerated after 48 ± 3 h incubation at 32°C. No statistical difference was observed between 3M Petrifilm RAC Plate and FDA BAM or SMEDP reference methods for each contamination level.

  10. Comparison of 3M Petrifilm Aerobic Count Plates to standard plating methodology for use with AOAC antimicrobial efficacy methods 955.14, 955.15, 964.02, and 966.04 as an alternative enumeration procedure: collaborative study.

    Science.gov (United States)

    Nelson, Maria T; LaBudde, Robert A; Tomasino, Stephen F; Pines, Rebecca M

    2013-01-01

    A multilaboratory study was conducted to determine the equivalence of the 3M Petrifilm Aerobic Count Plate and standard plating methodology for measuring viable bacteria and spores recovered from hard-surface carriers (stainless steel and porcelain), also known as "control carrier counts," used in AOAC antimicrobial efficacy test methods. Six laboratories participated in the study in which carriers inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, and spores of Bacillus subtilis were evaluated using 3M Petrifilm Aerobic Count (AC) plates and standard plating side-by-side. The data were analyzed using a matched-pair t-test to determine the between-method effect with confidence intervals. For all test organisms pooled across all laboratories, the mean difference in log10 concentration between the standard plate count method and 3M Petrifilm AC Plates was -0.012, with a 95% confidence interval of (-0.090, +0.066), which was well within the -0.5, +0.5 interval established as the acceptance criterion. The between-carrier SD averaged 0.139; the between-replicate SD was 0.050. The carrier reproducibility, given that a single replicate per carrier is done, was estimated to be 0.148. Although differences were seen in the final concentrations of the test organisms among laboratories, there were no statistical differences between the enumeration methods. Based on the results from this study, 3M Petrifilm AC Plates are equivalent to standard plating methodology and can be used as an alternative procedure for the enumeration of test organisms used in AOAC Methods 955.14, 955.15, 964.02, and 966.04.

  11. Lack of correlation between Legionella colonization and microbial population quantification using heterotrophic plate count and adenosine triphosphate bioluminescence measurement.

    Science.gov (United States)

    Duda, Scott; Baron, Julianne L; Wagener, Marilyn M; Vidic, Radisav D; Stout, Janet E

    2015-07-01

    This investigation compared biological quantification of potable and non-potable (cooling) water samples using pour plate heterotrophic plate count (HPC) methods and adenosine triphosphate (ATP) concentration measurement using bioluminescence. The relationship between these measurements and the presence of Legionella spp. was also examined. HPC for potable and non-potable water were cultured on R2A and PCA, respectively. Results indicated a strong correlation between HPC and ATP measurements in potable water (R = 0.90, p ATP and HPC were much weaker but statistically significant (make-up water: R = 0.37, p = 0.005; cooling tower 1: R = 0.52, p ATP. However, ATP measurements showed higher microbial concentrations than HPC measurements. Following chlorination of the cooling towers, ATP measurements indicated very low bacterial concentrations (1000 CFU/mL) which consisted primarily of non-tuberculous mycobacteria. HPC concentrations have been suggested to be predictive of Legionella presence, although this has not been proven. Our evaluation showed that HPC or ATP demonstrated a fair predictive capacity for Legionella positivity in potable water (HPC: receiver operating characteristic (ROC) = 0.70; ATP: ROC = 0.78; p = 0.003). However, HPC or ATP correctly classified sites as positive only 64 and 62% of the time, respectively. No correlation between HPC or ATP and Legionella colonization in non-potable water samples was found (HPC: ROC = 0.28; ATP: ROC = 0.44; p = 0.193).

  12. 3M Petrifilm Staph Express Count plate method for the enumeration of Staphylococcus aureus in selected types of meat, seafood, and poultry: collaborative study.

    Science.gov (United States)

    McMahon, Wendy A; Aleo, Victoria A; Schultz, Ann M; Horter, Barbara L; Lindberg, Kathryn G

    2003-01-01

    The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method 975.55 for the enumeration of Staphylococcus aureus in selected foods. Four foods--cooked, diced chicken; cured ham; smoked salmon; and pepperoni--were analyzed for S. aureus by 12 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample, a low inoculation level, a medium inoculation level, and a medium inoculation level with background flora, each in duplicate. The mean log10 counts for the methods were comparable for all 4 foods. The repeatability and reproducibility variances of the 24 h Petrifilm Staph Express Count plate method were similar to those of the 72 h standard method.

  13. 3M Petrifilm Staph Express Count plate method for the enumeration of Staphylococcus aureus in selected types of processed and prepared foods: collaborative study.

    Science.gov (United States)

    Silbernagel, Karen M; Jechorek, Robert P; Carver, Charles N; Horter, Barbara L; Lindberg, Kathryn G

    2003-01-01

    The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method 975.55 for the enumeration of Staphylococcus aureus in selected foods. Five foods--frozen lasagna, custard, frozen mixed vegetables, frozen hashbrowns, and frozen batter-coated mushrooms--were analyzed for S. aureus by 13 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample, a low inoculation level, a medium inoculation level, and a medium inoculation level with background flora, each in duplicate. The mean log10 counts for the methods were comparable for all 5 foods. The repeatability and reproducibility variances of the 24 h Petrifilm Staph Express Count plate method were similar to those of the 72 h standard method.

  14. VAPI: low-cost, rapid automated visual inspection system for Petri plate analysis

    Science.gov (United States)

    Chatburn, L. T.; Kirkup, B. C.; Polz, M. F.

    2007-09-01

    Most culture-based microbiology tasks utilize a petri plate during processing, but rarely do the scientists capture the full information available from the plate. In particular, visual analysis of plates is an under-developed rich source of data that can be rapid and non-invasive. However, collecting this data has been limited by the difficulties of standardizing and quantifying human observations, by the limits of a scientists' fatigue, and by the cost of automating the process. The availability of specialized counting equipment and intelligent camera systems has not changed this - they are prohibitively expensive for many laboratories, only process a limited number of plate types, are often destructive to the sample, and have limited accuracy. This paper describes an automated visual inspection solution, VAPI, that employs inexpensive consumer computing hardware and digital cameras along with custom cross-platform open-source software written in C++, combining Trolltech's Qt GUI toolkit with Intel's OpenCV computer vision library. The system is more accurate than common commercial systems costing many times as much, while being flexible in use and offering comparable responsiveness. VAPI not only counts colonies but also sorts and enumerates colonies by morphology, tracks colony growth by time series analysis, and provides other analytical resources. Output to XML files or directly to a database provides data that can be easily maintained and manipulated by the end user, offering ready access for system enhancement, interaction with other software systems, and rapid development of advanced analysis applications.

  15. Analysis of electroperforated materials using the quadrat counts method

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, E; Garzon, C; Garcia-Garcia, J [Departament d' Enginyeria Electronica, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); MartInez-Cisneros, C; Alonso, J, E-mail: enrique.miranda@uab.cat [Departament de Quimica AnalItica, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain)

    2011-06-23

    The electroperforation distribution in thin porous materials is investigated using the quadrat counts method (QCM), a classical statistical technique aimed to evaluate the deviation from complete spatial randomness (CSR). Perforations are created by means of electrical discharges generated by needle-like tungsten electrodes. The objective of perforating a thin porous material is to enhance its air permeability, a critical issue in many industrial applications involving paper, plastics, textiles, etc. Using image analysis techniques and specialized statistical software it is shown that the perforation locations follow, beyond a certain length scale, a homogeneous 2D Poisson distribution.

  16. Finite Element Analysis of Circular Plate using SolidWorks

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Yeo Jin; Jhung, Myung Jo [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

    2011-10-15

    Circular plates are used extensively in mechanical engineering for nuclear reactor internal components. The examples in the reactor vessel internals are upper guide structure support plate, fuel alignment plate, lower support plate etc. To verify the structural integrity of these plates, the finite element analyses are performed, which require the development of the finite element model. Sometimes it is very costly and time consuming to make the model especially for the beginners who start their engineering job for the structural analysis, necessitating a simple method to develop the finite element model for the pursuing structural analysis. Therefore in this study, the input decks are generated for the finite element analysis of a circular plate as shown in Fig. 1, which can be used for the structural analysis such as modal analysis, response spectrum analysis, stress analysis, etc using the commercial program Solid Works. The example problems are solved and the results are included for analysts to perform easily the finite element analysis of the mechanical plate components due to various loadings. The various results presented in this study would be helpful not only for the benchmark calculations and results comparisons but also as a part of the knowledge management for the future generation of young designers, scientists and computer analysts

  17. FINITE ELEMENT STRUCTURAL & THERMAL ANALYSIS OF LOCK PLATE

    OpenAIRE

    Mr. Ketan Patil; Mr. Roshan Marode; Prof. V. M. Chidri; Prof. A.J. Keche

    2012-01-01

    In Centrifugal Pipe Casting machine, hot molten metal is injected from one end and the other end remains closed by a covering plate having standard dimension. This covering plate is locked by a worker with the help of a spanner. This covering plate again needs to be opened after completion of casting process for the extraction of the pipe from the mould. This paper discuss mainly focused on the analysis of locking plate and its new design so that the humaninterference must be reduced to highe...

  18. Nonlinear analysis of flexible plates lying on elastic foundation

    Directory of Open Access Journals (Sweden)

    Trushin Sergey

    2017-01-01

    Full Text Available This article describes numerical procedures for analysis of flexible rectangular plates lying on elastic foundation. Computing models are based on the theory of plates with account of transverse shear deformations. The finite difference energy method of discretization is used for reducing the initial continuum problem to finite dimensional problem. Solution procedures for nonlinear problem are based on Newton-Raphson method. This theory of plates and numerical methods have been used for investigation of nonlinear behavior of flexible plates on elastic foundation with different properties.

  19. Flow Field Analysis of Submerged Horizontal Plate Type Breakwater

    Institute of Scientific and Technical Information of China (English)

    张志强; 栾茂田; 王科

    2013-01-01

    Submerged horizontal plate can be considered as a new concept breakwater. In order to reveal the wave elimination mechanism of this type breakwater, boundary element method is utilized to investigate the velocity field around plate carefully. The flow field analysis shows that the interaction between incident wave and reverse flow caused by submerged plate will lead to the formation of wave elimination area around both sides of the plate. The velocity magnitude of flow field has been reduced and this is the main reason of wave elimination.

  20. Analysis of BY-106 pump pit cover plate

    Energy Technology Data Exchange (ETDEWEB)

    Coverdell, B.L.

    1994-11-14

    A new cover for the pump pit of Tank 241-BY-106 has been designed to allow the rotary core exhauster to be hooked up without requiring pit entry, riser modification, or equipment removal. The new pit cover is necessary to allow installation of two risers for reducing exposure, contamination, and waste. Computer analysis indicates that the safety margin of the pit cover plate with two risers is adequate. The computer stress model and input files are attached. The pit cover plate is a replacement for an existing plate; therefore seismic and wind loads were considered for the plate only.

  1. Free vibration analysis of rectangular plates with central cutout

    Directory of Open Access Journals (Sweden)

    Kanak Kalita

    2016-12-01

    Full Text Available A nine-node isoparametric plate element in conjunction with first-order shear deformation theory is used for free vibration analysis of rectangular plates with central cutouts. Both thick and thin plate problems are solved for various aspect ratios and boundary conditions. In this article, primary focus is given to the effect of rotary inertia on natural frequencies of perforated rectangular plates. It is found that rotary inertia has significant effect on thick plates, while for thin plates the rotary inertia term can be ignored. It is seen that the numerical convergence is very rapid and based on comparison with experimental and analytical data from literature, it is proposed that the present formulation is capable of yielding highly accurate results. Finally, some new numerical solutions are provided here, which may serve as benchmark for future research on similar problems.

  2. Evaluation of read count based RNAseq analysis methods.

    Science.gov (United States)

    Guo, Yan; Li, Chung-I; Ye, Fei; Shyr, Yu

    2013-01-01

    RNAseq technology is replacing microarray technology as the tool of choice for gene expression profiling. While providing much richer data than microarray, analysis of RNAseq data has been much more challenging. To date, there has not been a consensus on the best approach for conducting robust RNAseq analysis. In this study, we designed a thorough experiment to evaluate six read count-based RNAseq analysis methods (DESeq, DEGseq, edgeR, NBPSeq, TSPM and baySeq) using both real and simulated data. We found the six methods produce similar fold changes and reasonable overlapping of differentially expressed genes based on p-values. However, all six methods suffer from over-sensitivity. Based on the evaluation of runtime using real data and area under the receiver operating characteristic curve (AUC-ROC) using simulated data, we found that edgeR achieves a better balance between speed and accuracy than the other methods.

  3. Moire interferometry for vibration analysis of plates

    Science.gov (United States)

    Asundi, A.; Cheung, M. T.

    1987-12-01

    Moire interferometry is used to locate nodal regions and measure vibration amplitudes of sinusoidally vibrating square plates. The high sensitivity afforded by this technique makes possible the study of plate vibrations at high frequencies and low amplitudes. The initial pattern is modulated by the zero-order Bessel function representing the vibratory motion. The fringe (or fringes) with best contrast indicate the nodal regions, while the higher order fringes, describing loci of points vibrating with the same amplitude, have decreasing contrast which is improved by spatial filtering.

  4. A lumped mass finite element method for vibration analysis of elastic plate-plate structures

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The fully discrete lumped mass finite element method is proposed for vibration analysis of elastic plate-plate structures.In the space directions,the longitudinal displacements on plates are discretized by conforming linear elements,and the transverse displacements are discretized by the Morley element.By means of the second order central difference for discretizing the time derivative and the technique of lumped masses,a fully discrete lumped mass finite element method is obtained,and two approaches to choosing the initial functions are also introduced.The error analysis for the method in the energy norm is established,and some numerical examples are included to validate the theoretical analysis.

  5. Resistive Plate Chamber Efficiency & Rate Capability Analysis

    Science.gov (United States)

    Candocia, Max

    2012-10-01

    Bakelite-based resistive plate chambers (RPCs) are particle detectors commonly used in muon trigger systems for high-energy physics experiments. Bakelite RPCs combine fast response, sufficient position resolution and low cost, and they can be operated at instantaneous background rates up to approximately 1.5 kHz/cm^2. Current and future collider experiments will demand operation of trigger RPCs under background rates higher than what is currently achieved. The rate capability is related to the bulk and surface conductivities of the Bakelite material used for the plates bordering the active gas volume in the RPCs. At the LHC and RHIC, these surfaces are coated with linseed oil, which lowers the surface resistivity of the Bakelite, which, to a point, improves the rate capability of the detectors. We have doped our own plates with various concentrations of carbon black. Over the past year we have tested RPCs with Bakelite plates of different resistivity using cosmic ray muons and radioactive Fe55 sources to emulate different levels of background in the detector. Results on the RPC efficiencies at different background rates and for different Bakelite coatings will be presented.

  6. Biomechanical analysis of titanium fixation plates and screws in ...

    African Journals Online (AJOL)

    Key words: Bone plates, bone screws, finite element analysis, jaw fixation techniques, mandible, mandibular .... model is built up, in similar way to building block construction, .... shows advantages, such as granting intraoral route, minimal.

  7. Asymptotic analysis of laminated plates and shallow shells

    Science.gov (United States)

    Skoptsov, K. A.; Sheshenin, S. V.

    2011-02-01

    It was noted long ago [1] that the material strength theory develops both by improving computational methods and by widening the physical foundations. In the present paper, we develop a computational technique based on asymptotic methods, first of all, on the homogenization method [2, 3]. A modification of the homogenization method for plates periodic in the horizontal projection was proposed in [4], where the bending of a homogeneous plate with periodically repeating inhomogeneities on its surface was studied. A more detailed asymptotic analysis of elastic plates periodic in the horizontal projection can be found, e.g., in [5, 6]. In [6], three asymptotic approximations were considered, local problems on the periodicity cell were obtained for them, and the solvability of these problems was proved. In [7], it was shown that the techniques developed for plates periodic in the horizontal projection can also be used for laminated plates. In [7], this was illustrated by an example of asymptotic analysis of an isotropic plate symmetric with respect to the midplane. In what follows, these methods are generalized to the case of combined bending and extension of a longitudinal laminated plate up to the third approximation, which permits finding all components of the stress tensor. The study of the plate behavior is based on the method of homogenization of the three-dimensional problem of linear elasticity and does not use any hypotheses. It turns out that the Kirchhoff-Love hypothesis for the entire packet of layers is simply a consequence of the method in the zeroth approximation, and the bending stresses corresponding to the classical theory of laminated plates [8] are obtained in the first approximation. The successive approximations describe the behavior of the normal and the stress more precisely. In the present paper, the results obtained in [7] are refined, and the asymptotic solution is compared with the direct analysis of a laminated plate by the finite

  8. Thermal Analysis of Thin Plates Using the Finite Element Method

    Science.gov (United States)

    Er, G. K.; Iu, V. P.; Liu, X. L.

    2010-05-01

    The isotropic thermal plate is analyzed with finite element method. The solution procedure is presented. The elementary stiffness matrix and loading vector are derived rigorously with variation principle and the principle of minimum potential energy. Numerical results are obtained based on the derived equations and tested with available exact solutions. The problems in the finite element analysis are figured out. It is found that the finite element solutions can not converge as the number of elements increases around the corners of the plate. The derived equations presented in this paper are fundamental for our further study on more complicated thermal plate analysis.

  9. Analysis of Flat-Plate Solar Array and Solar Lantern

    Directory of Open Access Journals (Sweden)

    P. L. N. V. Aashrith

    2014-05-01

    Full Text Available A very detailed theortical analysis of a solar array has been carried out based on established values of solar radiation data to predict the performance of solar lamp . The analysis is based on established theory about flat-plate collectors. Top heat loss coefficient (Ut, Bottom heat loss coefficient (Ub, Overall heat loss coefficient (Ul, Useful energy (Qu, efficiency (hp of the flat-plate solar array and efficiency (hl of the solar lantern has been calculated.

  10. Noise-free accurate count of microbial colonies by time-lapse shadow image analysis.

    Science.gov (United States)

    Ogawa, Hiroyuki; Nasu, Senshi; Takeshige, Motomu; Funabashi, Hisakage; Saito, Mikako; Matsuoka, Hideaki

    2012-12-01

    Microbial colonies in food matrices could be counted accurately by a novel noise-free method based on time-lapse shadow image analysis. An agar plate containing many clusters of microbial colonies and/or meat fragments was trans-illuminated to project their 2-dimensional (2D) shadow images on a color CCD camera. The 2D shadow images of every cluster distributed within a 3-mm thick agar layer were captured in focus simultaneously by means of a multiple focusing system, and were then converted to 3-dimensional (3D) shadow images. By time-lapse analysis of the 3D shadow images, it was determined whether each cluster comprised single or multiple colonies or a meat fragment. The analytical precision was high enough to be able to distinguish a microbial colony from a meat fragment, to recognize an oval image as two colonies contacting each other, and to detect microbial colonies hidden under a food fragment. The detection of hidden colonies is its outstanding performance in comparison with other systems. The present system attained accuracy for counting fewer than 5 colonies and is therefore of practical importance.

  11. Cautions on the Use of National Standard Method for Aerobic Plate Count in Food and Rapid Detection Techniques for Aerobic Plate Count%食品中茵落总数的国标法测定注意事项及快速检测技术

    Institute of Scientific and Technical Information of China (English)

    黄维雅

    2012-01-01

    菌落总数是食品微生物检测的一个重要项目,也是评价食品卫生安全性最常用的指标之一,其结果的准确度和可信度直接影响评价的可靠性。该文总结了食品中菌落总数的国标法检测注意事项,并介绍了近年来常用的几种食品菌落总数的快速检测技术。%The aerobic plate count is important in the food microbiological examination and widely used in evaluation of food hygiene and safety. The reliability of evaluations is depended on the accuracy and the credibility of the aerobic plate count. This paper summarized some cautions on the use of national standard method for aerobic plate count in food microbiological examination, and reviewed some common rapid detection techniques for aerobic plate count in food microbiological study in recent years.

  12. Stiffness Analysis and Improvement of Bolt-Plate Contact Assemblies

    DEFF Research Database (Denmark)

    Pedersen, Niels Leergaard; Pedersen, Pauli

    2008-01-01

    In a previous study it was shown that, a simplified expression for the stiffness of the plate member in a bolt-plate assembly can be found. The stiffnesses of the bolt and the connected plates are the primary quantities that control the lifetime of a dynamically loaded connection. The present study...... of stiffnesses is extended to include different material parameters by including the influence of Poisson's ratio. Two simple practical formulas are suggested and their accuracies are documented for different bolts and different material (Poisson's ratio). Secondly, the contact analysis between the bolt head...... and the plate is extended by the possibility of designing a gap, that is, a nonuniform distance between the bolt and plate before prestressing. Designing the gap function generates the possibility for a better stress field by which the stiffness of the bolt is lowered, and at the same time the stiffness...

  13. Vibration Analysis of Annular Sector Plates under Different Boundary Conditions

    Directory of Open Access Journals (Sweden)

    Dongyan Shi

    2014-01-01

    Full Text Available An analytical framework is developed for the vibration analysis of annular sector plates with general elastic restraints along each edge of plates. Regardless of boundary conditions, the displacement solution is invariably expressed as a new form of trigonometric expansion with accelerated convergence. The expansion coefficients are treated as the generalized coordinates and determined using the Rayleigh-Ritz technique. This work allows a capability of modeling annular sector plates under a variety of boundary conditions and changing the boundary conditions as easily as modifying the material properties or dimensions of the plates. Of equal importance, the proposed approach is universally applicable to annular sector plates of any inclusion angles up to 2π. The reliability and accuracy of the current method are adequately validated through numerical examples.

  14. Biomechanical analysis of limited-contact plate used for osteosynthesis.

    Science.gov (United States)

    Pochrząst, Magdalena; Basiaga, Marcin; Marciniak, Jan; Kaczmarek, Marcin

    2014-01-01

    This paper presents the results of numerical analysis aimed at determining the state of stresses and displacements of compression plate used in osteosynthesis of tibia, carried out by applying finite element method using the ANSYS program. The analysis took into account two variants of the osteosynthesis. Variant I included the osteosynthesis in which plate was attached directly to the bone, in variant II, the plate was moved away from the bones by about 5 mm. Biomechanical characteristics of the corrective osteotomy plate-tibia was determined for implants made of Ti-6Al-4V alloy. The boundary conditions adopted for the analysis reflect phenomena occurring in a real system. Based on the results of the analysis relative displacements and reduced stresses in various components were determined as a function of the applied load within the range of F = 500-1500 N. The maximum forces, both variant I and variant II determined during analysis, ensure that the generated stress does not exceed yield strength of the material and compressive strength of the bone, and do not exceed safety movement in the fracture gap. In addition, it was found that the locking of the compressive plate to the bone has a little effect on the distribution of displacements and stresses on the plate-tibia system in the case of a simple fracture.

  15. Buckling analysis of sandwich plate using layer wise theory

    Energy Technology Data Exchange (ETDEWEB)

    Ranjbaran, Arash; Khoshravan, Mohammad Reza [University of Tabriz, Tabriz (Iran, Islamic Republic of); Kharazi, Mahsa [Sahand University of Technology, Sahand (Iran, Islamic Republic of)

    2014-07-15

    Buckling analysis of sandwich plate was investigated using layer wise method. The formulation was based on the first-order shear deformation theory, and the Rayleigh-Ritz method was used for approximating and determining the displacement field. The results obtained from layer wise theory was compared with finite element results and showed good agreement. This study demonstrated that layer wise theory could describe buckling behavior of sandwich plates with high accuracy and represents a more realistic and acceptable description of behavior of the plates with much less computational cost.

  16. LIRA: Low-Count Image Reconstruction and Analysis

    Science.gov (United States)

    Stein, Nathan; van Dyk, David; Connors, Alanna; Siemiginowska, Aneta; Kashyap, Vinay

    2009-09-01

    LIRA is a new software package for the R statistical computing language. The package is designed for multi-scale non-parametric image analysis for use in high-energy astrophysics. The code implements an MCMC sampler that simultaneously fits the image and the necessary tuning/smoothing parameters in the model (an advance from `EMC2' of Esch et al. 2004). The model-based approach allows for quantification of the standard error of the fitted image and can be used to access the statistical significance of features in the image or to evaluate the goodness-of-fit of a proposed model. The method does not rely on Gaussian approximations, instead modeling image counts as Poisson data, making it suitable for images with extremely low counts. LIRA can include a null (or background) model and fit the departure between the observed data and the null model via a wavelet-like multi-scale component. The technique is therefore suited for problems in which some aspect of an observation is well understood (e.g, a point source), but questions remain about observed departures. To quantitatively test for the presence of diffuse structure unaccounted for by a point source null model, first, the observed image is fit with the null model. Second, multiple simulated images, generated as Poisson realizations of the point source model, are fit using the same null model. MCMC samples from the posterior distributions of the parameters of the fitted models can be compared and can be used to calibrate the misfit between the observed data and the null model. Additionally, output from LIRA includes the MCMC draws of the multi-scale component images, so that the departure of the (simulated or observed) data from the point source null model can be examined visually. To demonstrate LIRA, an example of reconstructing Chandra images of high redshift quasars with jets is presented.

  17. AUTOMATIC LICENSE PLATE LOCALISATION AND IDENTIFICATION VIA SIGNATURE ANALYSIS

    Directory of Open Access Journals (Sweden)

    Lorita Angeline

    2014-02-01

    Full Text Available A new algorithm for license plate localisation and identification is proposed on the basis of Signature analysis. Signature analysis has been used to locate license plate candidate and its properties can be further utilised in supporting and affirming the license plate character recognition. This paper presents Signature Analysis and the improved conventional Connected Component Analysis (CCA to design an automatic license plate localisation and identification. A procedure called Euclidean Distance Transform is added to the conventional CCA in order to tackle the multiple bounding boxes that occurred. The developed algorithm, SAICCA achieved 92% successful rate, with 8% failed localisation rate due to the restrictions such as insufficient light level, clarity and license plate perceptual information. The processing time for a license plate localisation and recognition is a crucial criterion that needs to be concerned. Therefore, this paper has utilised several approaches to decrease the processing time to an optimal value. The results obtained show that the proposed system is capable to be implemented in both ideal and non-ideal environments.

  18. 培养条件对海水中菌落总数测定的影响%Effects of culture condition on aerobic plate count of seawater

    Institute of Scientific and Technical Information of China (English)

    谢昭聪; 郑文丽; 徐立俊; 李小燕; 黄明燕; 袁新竹; 杜林

    2011-01-01

    目的 了解平板计数法测定海水中菌落总数时适宜的培养条件:培养基、培养温度和培养时间.方法 深圳水域的海水样品在不同培养基、培养温度条件下,经过不同培养时间培养后进行菌落总数计数.结果 在培养温度为25t,3090,36℃条件下,在相同培养时间内,2216E琼脂的菌落总数明显高于营养琼脂的菌落总数及平板计数琼脂的菌落总数(P < 0.05);采用2216E琼脂进行菌落总数测定,在培养温度30℃培养96h后,菌落总数达到最高,且菌落总数高于25℃和36℃培养时的菌落总数(P < 0.05).结论 海水中菌落总数测定时应注意培养时间的控制.2216E琼脂适合用于海水菌落总数测定.深圳区域海水中菌落总数测定适宜采用2216E琼脂于30℃培养96h.有关国家标准中海水中菌落总数测定方法所规定的培养温度、培养时间应考虑进行修订完善以准确检测水温较高的南方区域海水.%Objective To study studied the suitable medium, culture temperature and culture time, and to determine the aerobic plate count of seawater. Methods Samples of seawater from Shenzhen area were cultured in different media, temperature, after different time colony-forming unit were counted. Results In culture temperature of 25℃, 30℃, 36℃, with the same culture time, colonies numbers on 2216E agar were obviously more than on the nutrient agar and the plate count agar (P < 0.05); using 2216E agar for aerobic plate count in temperature 30℃ reached the highest number of colonies after 96 h, and the numbers of colony are higher than in 25℃ and 36℃ (P < 0.05). Conclusion Culture time should be well controlled when the aerobic plate count of seawater was detected. 2216E agar is a suitable medium for aerobic plate count of seawater in Shenzhen area. In order to accurately detect the aerobic plate count of seawater of the South region where water temperature is relatively high, the necessary amendments to

  19. Stochastic analysis of laminated composite plate considering stochastic homogenization problem

    Institute of Scientific and Technical Information of China (English)

    S. SAKATA; K. OKUDA; K. IKEDA

    2015-01-01

    This paper discusses a multiscale stochastic analysis of a laminated composite plate consisting of unidirectional fiber reinforced composite laminae. In particular, influence of a microscopic random variation of the elastic properties of component materials on mechanical properties of the laminated plate is investigated. Laminated composites are widely used in civil engineering, and therefore multiscale stochastic analysis of laminated composites should be performed for reliability evaluation of a composite civil structure. This study deals with the stochastic response of a laminated composite plate against the microscopic random variation in addition to a random variation of fiber orientation in each lamina, and stochastic properties of the mechanical responses of the laminated plate is investigated. Halpin-Tsai formula and the homogenization theory-based finite element analysis are employed for estimation of effective elastic properties of lamina, and the classical laminate theory is employed for analysis of a laminated plate. The Monte-Carlo simulation and the first-order second moment method with sensitivity analysis are employed for the stochastic analysis. From the numerical results, importance of the multiscale stochastic analysis for reliability evaluation of a laminated composite structure and applicability of the sensitivity-based approach are discussed.

  20. Finite Element Vibration Analysis of Beams, Plates and Shells

    Directory of Open Access Journals (Sweden)

    Jaroslav Mackerle

    1999-01-01

    Full Text Available This bibliography lists references to papers, conference proceedings and theses/dissertations dealing with finite element vibration analysis of beams, plates and shells that were published in 1994–1998. It contains 361 citations. Also included, as separated subsections, are vibration analysis of composite materials and vibration analysis of structural elements with cracks/contacts.

  1. Design Modification & Failure Analysis of a Driven Plate/Friction Plate of a Clutch using FEA.

    Directory of Open Access Journals (Sweden)

    Marella. Veerendra

    2014-10-01

    Full Text Available Clutch is device which is used to transmit power one component to another. It is a device used to connect the driving shaft to a driven shaft, so that the driven shaft may be started or stopped at will, without stopping the driving shaft. A clutch thus provides an interruptible connection between two rotating shafts Clutches allow a high inertia load to be stated with a small power. The engine power transmitted to the system through the clutch. The failure of such a critical component during service can stall the whole application. The objective of present project is to do analysis on driven plate of a clutch which is often fails during the operation. In this present research work analysis is conducted on driven plate of a clutch used in TATA Vehicle. There are so many complaints are noted against the failure of clutch during heavy loads. The driven main plate failed normally during its operation due to cyclic loading. For this reason we designed the driven plate and modeled in Pro-E and analyzed using FEA package in the process of designing and analyzing the actual design is changed to lower down the failure values. And the design may suggest to the company.

  2. PLATE

    DEFF Research Database (Denmark)

    Kling, Joyce; Hjulmand, Lise-Lotte

    2008-01-01

    ’s level of English is sufficient for the increasing number of courses offered in English each semester. This paper addresses these concerns and describes a pilot project initiated in 2003 at CBS to gauge the overall English language proficiency of those teaching content courses in English. Through......Copenhagen Business School (CBS) finds itself needing to address the issue of English-medium instruction for its increasing number of foreign exchange and full degree students. With internationalisation as a main pillar of the institution’s agenda, there are concerns whether the teaching faculty...... the Project in Language Assessment for Teaching in English (PLATE) language professionals from CBS’s Language Center observe teachers and provide feedback using evaluation criteria from the Common European Framework for Reference (CEFR) supplemented by some additional criteria which take the LSP nature...

  3. PLATE

    DEFF Research Database (Denmark)

    Kling, Joyce; Hjulmand, Lise-Lotte

    2008-01-01

    Copenhagen Business School (CBS) finds itself needing to address the issue of English-medium instruction for its increasing number of foreign exchange and full degree students. With internationalisation as a main pillar of the institution’s agenda, there are concerns whether the teaching faculty......’s level of English is sufficient for the increasing number of courses offered in English each semester. This paper addresses these concerns and describes a pilot project initiated in 2003 at CBS to gauge the overall English language proficiency of those teaching content courses in English. Through...... the Project in Language Assessment for Teaching in English (PLATE) language professionals from CBS’s Language Center observe teachers and provide feedback using evaluation criteria from the Common European Framework for Reference (CEFR) supplemented by some additional criteria which take the LSP nature...

  4. Evaluation of the petrifilm aerobic count plate for enumeration of aerobic marine bacteria from seawater and Caulerpa lentillifera

    National Research Council Canada - National Science Library

    Kudaka, Jun; Horii, Toru; Tamanaha, Koji; Itokazu, Kiyomasa; Nakamura, Masaji; Taira, Katsuya; Nidaira, Minoru; Okano, Sho; Kitahara, Akio

    2010-01-01

    ... and an edible seaweed, Caulerpa lentillifera. The efficiency of enumeration of total aerobic marine bacteria on Petrifilm ACP was compared with that using the spread plate method on marine agar with 80...

  5. Free vibration Analysis of Sandwich Plates with cutout

    Science.gov (United States)

    Mishra, N.; Basa, B.; Sarangi, S. K.

    2016-09-01

    This paper presents the free vibration analysis of sandwich plates with cutouts. Cutouts are inevitable in structural applications and the presence of these cutouts in the structures greatly influences their dynamic characteristics. A finite element model has been developed here using the ANSYS 15.0 software to study the free vibration characteristics of sandwich plates in the presence of cutouts. Shell 281 element, an 8-noded element with six degrees of freedom suited for analyzing thin to moderately thick structures is considered in the development of the model. Block Lanczose method is adopted to extract the mode shapes to obtain the natural frequency corresponding to free vibration of the plate. The effects of parametric variation on the natural frequency of the sandwich plates with cutout are studied and results are presented.

  6. ANALYSIS OF GLOBAL DYNAMICS IN A PAIAMETIICALLY EXCITED THIN PLATE

    Institute of Scientific and Technical Information of China (English)

    张伟

    2001-01-01

    The global bifurcations and chaos of a simply supported rectangular thin plate with parametric excitation are analyzed. The formulas of the thin plate are derived by yon Karman type equation and Galerkin's approach. The method of multiple scales is used to obtain the averaged equations. Based on the averaged equations, the theory of the normal form is used to give the explicit expressions of the normal form associated with a double zero and a pair of pure imaginary eigenvalues by Maple program. On the basis of the normal form, a global bifurcation analysis of the parametrically excited recta ngular thin plate is given by the global perturbation method developed by Kovacic and Wiggins. The chaotic motion of thin plate is also found by numerical simulation.

  7. Vibration analysis of composite laminate plate excited by piezoelectric actuators.

    Science.gov (United States)

    Her, Shiuh-Chuan; Lin, Chi-Sheng

    2013-01-01

    Piezoelectric materials can be used as actuators for the active vibration control of smart structural systems. In this work, piezoelectric patches are surface bonded to a composite laminate plate and used as vibration actuators. A static analysis based on the piezoelectricity and elasticity is conducted to evaluate the loads induced by the piezoelectric actuators to the host structure. The loads are then employed to develop the vibration response of a simply supported laminate rectangular plate excited by piezoelectric patches subjected to time harmonic voltages. An analytical solution of the vibration response of a simply supported laminate rectangular plate under time harmonic electrical loading is obtained and compared with finite element results to validate the present approach. The effects of location and exciting frequency of piezoelectric actuators on the vibration response of the laminate plate are investigated through a parametric study. Numerical results show that modes can be selectively excited, leading to structural vibration control.

  8. Vibration Analysis of Plates by MLS-Element Method

    Science.gov (United States)

    Zhou, L.; Xiang, Y.

    2010-05-01

    This paper presents a novel numerical method, the moving least square element (MLS-element) method for the free vibration analysis of plates based on the Mindlin shear deformable plate theory. In the MLS-element method, a plate can be first divided into multiple elements which are connected through selected nodal points on the interfaces of the elements. An element can be of any shape and the size of the element varies dependent on the problem at hand. The shape functions of the element for the transverse displacement and the rotations are derived based on the MLS interpolation technique. The convergence and accuracy of the method can be controlled by either increasing the number of elements or by increasing the number of MLS interpolation points within elements. Two selected examples for vibration of a simply supported square Mindlin plate and a clamped L-shaped Mindlin plate are studied to illustrate the versatility and accuracy of the proposed method. It shows that the proposed method is highly accurate and flexible for the vibration analysis of plate problems. The method can be further developed to bridge the existing meshless method and the powerful finite element method in dealing with various engineering computational problems, such as large deformation and crack propagation in solid mechanics.

  9. Analysis of spatial count data using Kalman smoothing

    DEFF Research Database (Denmark)

    Dethlefsen, Claus

    2007-01-01

    at the location. We elaborate on a link between state space models and Markov random fields. The observations are modelled as independent Poisson counts conditional on a Gaussian Markov random field. We employ the fact that the model may be written as a state space model which may be analysed by combining......We consider spatial count data from an agricultural field experiment. Counts of weed plants in a field have been recorded in a project on precision farming. Interest is in mapping the weed intensity so that the dose of herbicide applied at any location can be adjusted to the amount of weed present...

  10. Analysis of spatial count data using Kalman smoothing

    DEFF Research Database (Denmark)

    Dethlefsen, Claus

    present at the location. We elaborate on a link between state space models and Markov random fields. The oberservations are modelled as independent Poisson counts conditional on a Gaussian Markov random field. We employ the fact that the model may be written as a state space model which may be analysed......This paper considers spatial count data from an agricultural field experiment. Counts of weed plants in a field have been recorded in a project on precision farming. Interest is in mapping the weed intensity so that the dose of herbicide applied at any location can be adjusted to the amount of weed...

  11. Application of microbiological method direct epifluorescence filter techique/aerobic plate count agar in the identification of irradiated herbs and spices

    Directory of Open Access Journals (Sweden)

    Maria Concetta Campagna

    2014-08-01

    Full Text Available Irradiation is a preservation technology used to improve the safety and hygienic quality of food. Aim of this study was to assess the applicability and validity of the microbiological screening method direct epifluorescence filter technique (DEFT/aerobic plate count (APC (EN 13783:2001 for the identification of irradiated herbs and spices. Tests on non-irradiated and irradiated samples of dried herbs and spices were performed. The method was based on the comparison of APC and count obtained using DEFT. In accordance with the standard reference, this method is not applicable to samples with APC<103 colony forming units (CFU/g and this is its main limit. The results obtained in our laboratories showed that in 50% of cases of non-irradiated samples and in 96% of the samples treated with ionising radiation, the method was not applicable due to a value of CFU/g<103.

  12. Application of Microbiological Method Direct Epifluorescence Filter Techique/Aerobic Plate Count Agar in the Identification of Irradiated Herbs and Spices

    Science.gov (United States)

    Di Schiavi, Maria Teresa; Foti, Marina; Mosconi, Maria Cristina; Mattiolo, Giuseppina; Cavallina, Roberta

    2014-01-01

    Irradiation is a preservation technology used to improve the safety and hygienic quality of food. Aim of this study was to assess the applicability and validity of the microbiological screening method direct epifluorescence filter technique (DEFT)/aerobic plate count (APC) (EN 13783:2001) for the identification of irradiated herbs and spices. Tests on non-irradiated and irradiated samples of dried herbs and spices were performed. The method was based on the comparison of APC and count obtained using DEFT. In accordance with the standard reference, this method is not applicable to samples with APC<103 colony forming units (CFU)/g and this is its main limit. The results obtained in our laboratories showed that in 50% of cases of non-irradiated samples and in 96% of the samples treated with ionising radiation, the method was not applicable due to a value of CFU/g <103. PMID:27800348

  13. Microfluidic Cytometer for Complete Blood Count Analysis Project

    Data.gov (United States)

    National Aeronautics and Space Administration — RMD proposes to develop a MEMS based complete blood count (CBC) instrument that can be used aboard a spacecraft. We will produce a microfluidic scale combination...

  14. Submillimeter Number Counts From Statistical Analysis of BLAST Maps

    CERN Document Server

    Patanchon, Guillaume; Bock, James J; Chapin, Edward L; Devlin, Mark J; Dicker, Simon R; Griffin, Matthew; Gundersen, Joshua O; Halpern, Mark; Hargrave, Peter C; Hughes, David H; Klein, Jeff; Marsden, Gaelen; Mauskopf, Philip; Moncelsi, Lorenzo; Netterfield, Calvin B; Olmi, Luca; Pascale, Enzo; Rex, Marie; Scott, Douglas; Semisch, Christopher; Thomas, Nicholas; Truch, Matthew D P; Tucker, Carole; Tucker, Gregory S; Viero, Marco P; Wiebe, Donald V

    2009-01-01

    We describe the application of a statistical method to estimate submillimeter galaxy number counts from the confusion limited observations of the Balloon-borne Large Aperture Submillimeter Telescope (BLAST). Our method is based on a maximum likelihood fit to the pixel histogram, sometimes called 'P(D)', an approach which has been used before to probe faint counts, the difference being that here we advocate its use even for sources with relatively high signal-to-noise ratios. This method has an advantage over standard techniques of source extraction in providing an unbiased estimate of the counts from the bright end down to flux densities well below the confusion limit. We specifically analyse BLAST observations of a roughly 10 sq. deg map centered on the Great Observatories Origins Deep Survey South field. We provide estimates of number counts at the three BLAST wavelengths, 250, 350, and 500 microns, instead of counting sources in flux bins we estimate the counts at several flux density nodes connected with ...

  15. Experimental Modal Analysis of a Flat Plate Subjected To Vibration

    Directory of Open Access Journals (Sweden)

    Owunna Ikechukwu

    2016-07-01

    Full Text Available Modal analysis is significant in evaluating the mode shapes generated by a component under vibrational excitation, as the mode shapes can be used to determine the displacement or response of the component under the influence vibration in real life application. Result obtained from the modal analysis will generate a number of resonances which the frequency and damping effect can be determined by measurement. However, determining the accuracy of modal analysis result is somewhat difficult as the experimental results and the results generated by Finite Element Analysis (FEA solvers can be affected by a number of factors pointed out in this paper. In this study, a flat plate was mounted on an electromagnetic shaker which enabled the excitation of the plate, while results of the response were measured using a transducer attached to the plate. The plate was also modelled using CATIA software and the files transferred to the different FEA solvers such as HYPERMESH, ANSYS 6 Degree of Freedom (DOF as well as ANSYS 5 degree of freedom, in which the same analysis was carried out to obtain a set of results other than the experimental results. Each FEA solver generated results that were in close proximity with the experimental results, particularly the results generated by ANSYS 5 Degree of freedom. Hence, to ascertain the accuracy of the results obtained from modal analysis experimental procedure, it is important to match up the results generated from different FEA solvers with the experimental results.

  16. REVIEW OF PERFORMANCE AND ANALYSIS ISI FLAT PLATE COLLECTOR WITH MODIFIED FLAT PLATE COLLECTOR

    Directory of Open Access Journals (Sweden)

    MR.Y.Y.NANDURKAR

    2012-03-01

    Full Text Available The market of solar water heater of natural circulation type (thermo-siphon is fast growing in India. Initial cost of the solar water heater system at present is high because of store type design. It is necessary to make the product more popular by reducing the cost. This is possible by reducing area of liquid flat plate collector by increasing tube diameter and reducing riser length. Hence it is essential to make solar water heater in affordable range of the general public class. Present work is based on review of comparative performance and analysis of ISI flat plate collector with modified flat plat collector. The paper will be helpful for those who are working in the area of solar water heating system and their use in domestic areas.

  17. Risk factors associated with bulk tank standard plate count, bulk tank coliform count, and the presence of Staphylococcus aureus on organic and conventional dairy farms in the United States.

    Science.gov (United States)

    Cicconi-Hogan, K M; Gamroth, M; Richert, R; Ruegg, P L; Stiglbauer, K E; Schukken, Y H

    2013-01-01

    The purpose of this study was to assess the association of bulk tank milk standard plate counts, bulk tank coliform counts (CC), and the presence of Staphylococcus aureus in bulk tank milk with various management and farm characteristics on organic and conventional dairy farms throughout New York, Wisconsin, and Oregon. Data from size-matched organic farms (n=192), conventional nongrazing farms (n=64), and conventional grazing farms (n=36) were collected at a single visit for each farm. Of the 292 farms visited, 290 bulk tank milk samples were collected. Statistical models were created using data from all herds in the study, as well as exclusively for the organic subset of herds. Because of incomplete data, 267 of 290 herds were analyzed for total herd modeling, and 173 of 190 organic herds were analyzed for the organic herd modeling. Overall, more bulk tanks from organic farms had Staph. aureus cultured from them (62% of organic herds, 42% conventional nongrazing herds, and 43% of conventional grazing herds), whereas fewer organic herds had a high CC, defined as ≥50 cfu/mL, than conventional farms in the study. A high standard plate count (×1,000 cfu/mL) was associated with decreased body condition score of adult cows and decreased milk production in both models. Several variables were significant only in the model created using all herds or only in organic herds. The presence of Staph. aureus in the bulk tank milk was associated with fewer people treating mastitis, increased age of housing, and a higher percentage of cows with 3 or fewer teats in both the organic and total herd models. The Staph. aureus total herd model also showed a relationship with fewer first-lactation animals, higher hock scores, and less use of automatic takeoffs at milking. High bulk tank CC was related to feeding a total mixed ration and using natural service in nonlactating heifers in both models. Overall, attentive management and use of outside resources were useful with regard to CC

  18. Mixture Models for the Analysis of Repeated Count Data.

    NARCIS (Netherlands)

    van Duijn, M.A.J.; Böckenholt, U

    1995-01-01

    Repeated count data showing overdispersion are commonly analysed by using a Poisson model with varying intensity parameter. resulting in a mixed model. A mixed model with a gamma distribution for the Poisson parameter does not adequately fit a data set on 721 children's spelling errors. An

  19. Analysis on formulas of concrete plate under contact explosion

    Directory of Open Access Journals (Sweden)

    Wang Zheng

    2015-01-01

    Full Text Available Based on many test data of concrete plates of several thicknesses, this paper presents some new simple engineering formulas for computing critical thickness of scabbing and perforation, the front explosion diameter and the rear scabbing diameter of reinforced concrete plates under contact explosion. For the damage problem of a 20 cm thick concrete target under contact explosion of 1.25 kg TNT charge, the paper gives the numerical simulation results of LS-DYNA software for comparison. The damage zone of concrete plate in numerical simulation is determined by analysis of tension pressure resulted from shock wave reflection on the free boundary. And the numerical simulation results are in basic agreement on the results of these engineering formulas.

  20. Springback analysis of thermoplastic composite plates

    Directory of Open Access Journals (Sweden)

    Stavrovský V.

    2012-06-01

    Full Text Available Residual stresses, which are set in the fiber reinforced composites during the laminate curing in a closed form, lead to dimensional changes of composites after extracting from the form and cooling. One of these dimensional changes is called “springback” of the angle sections. Other dimensional changes are warpage of flat sections of composite or displacement of single layers of composite for example. In our case four different lay-ups were analysed (three symmetrical and one unsymmetrical. An analytical model which covers temperature changes, chemical shrinkage during curing and moisture change was used. Also a FEM analysis was done for predicting the springback, and both calculations were compared with the measured data from manufacturer.

  1. Buckling analysis of partially corroded steel plates with irregular surfaces

    Indian Academy of Sciences (India)

    Ahmad Rahbar-Ranji

    2014-04-01

    Corrosion is a long-term, inevitable process, lessens the thickness and load carrying capacity of structures. Old steel structures are more vulnerable to buckling, yielding and fracture due to corrosion. In lieu of a detailed analysis, average thickness assumption is employed for general type of corrosion. However, the estimation of load carrying capacity reduction of corroded structures typically need a much higher level of accuracy, since the actual corroded plates would have irregular surfaces. The objective of this article is to determine the effect of general corrosion on reduction of elastic buckling strength of both-sided partially corroded plates with irregular surfaces. Eigenvalue analysis using finite element method is employed for Euler stress calculation of corroded plates. The effects of different influential parameters are investigated and it is found that, aspect ratio of plate, location of corroded area, standard deviation of thickness diminution and concentration of corrosion have influence on reduction of elastic buckling strength. Reduction of elastic buckling strength is very sensitive to the amount of corrosion loss. The higher the amount of corrosion loss, the more reduction of elastic buckling strength.

  2. Conditional analysis of mixed Poisson processes with baseline counts: implications for trial design and analysis.

    Science.gov (United States)

    Cook, Richard J; Wei, Wei

    2003-07-01

    The design of clinical trials is typically based on marginal comparisons of a primary response under two or more treatments. The considerable gains in efficiency afforded by models conditional on one or more baseline responses has been extensively studied for Gaussian models. The purpose of this article is to present methods for the design and analysis of clinical trials in which the response is a count or a point process, and a corresponding baseline count is available prior to randomization. The methods are based on a conditional negative binomial model for the response given the baseline count and can be used to examine the effect of introducing selection criteria on power and sample size requirements. We show that designs based on this approach are more efficient than those proposed by McMahon et al. (1994).

  3. Analysis of structural hot-spot stress in orthotropic plates

    Institute of Scientific and Technical Information of China (English)

    Chen Ce; Ji Bohai; Xu Hanjiang

    2012-01-01

    On the basis of the actual steel deck structure of Taizhou Bridge, this paper carries out hot-spot stress analysis on some key spots by using the finite element model which simulates local structure of orthotropic steel bridge decks. A finite element model is established for local structure of orthotropic steel bridge decks, and in the analysis of linear elasticity of the structure, face load is employed to simulate the loads from vehicle wheels. Analysis results show that main stresses are relatively heavy at the joints between diaphragm plates, top plates and U-shaped ribs and the joints between diaphragm plates and U-shaped ribs. These joints shall be regarded as key points for hot-spot stress analysis. Different mesh densities are adopted in the finite element model and the main stresses at different hot spots are contrasted and linear extrapolation is carried out using extrapolation formulae. Results show that different mesh densities have different influences on the hot-spot stresses at the welded seams of U-shaped ribs. These influences shall be considered in calculation and analysis.

  4. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228

    Energy Technology Data Exchange (ETDEWEB)

    Aleissa, Khalid A.; Almasoud, Fahad I.; Islam, Mohammed S. [Atomic Energy Research Institute, King Abdul Aziz City for Science and Technology, P.O. Box 6086, Riyadh 11442 (Saudi Arabia); L' Annunziata, Michael F. [IAEA Expert, Montague Group, P.O. Box 5033, Oceanside, CA 92052-5033 (United States)], E-mail: mlannunziata@cox.net

    2008-12-15

    The activities of {sup 228}Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide {sup 228}Ac. The radium was pre-concentrated on MnO{sub 2} and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter {sup 228}Ra({sup 228}Ac), the daughter nuclide {sup 228}Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by {sup 228}Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9{+-}0.1% was measured for {sup 228}Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317{+-}0.013 cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1 g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of {sup 228}Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for {sup 228}Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure {sup 228}Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume

  5. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228.

    Science.gov (United States)

    Aleissa, Khalid A; Almasoud, Fahad I; Islam, Mohammed S; L'Annunziata, Michael F

    2008-12-01

    The activities of (228)Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide (228)Ac. The radium was pre-concentrated on MnO(2) and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter (228)Ra((228)Ac), the daughter nuclide (228)Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by (228)Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9+/-0.1% was measured for (228)Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317+/-0.013cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of (228)Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for (228)Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure (228)Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid

  6. A high resolution, high frame rate detector based on a microchannel plate read out with the Medipix2 counting CMOS pixel chip.

    CERN Document Server

    Mikulec, Bettina; McPhate, J B; Tremsin, A S; Siegmund, O H W; Clark, Allan G; CERN. Geneva

    2005-01-01

    The future of ground-based optical astronomy lies with advancements in adaptive optics (AO) to overcome the limitations that the atmosphere places on high resolution imaging. A key technology for AO systems on future very large telescopes are the wavefront sensors (WFS) which detect the optical phase error and send corrections to deformable mirrors. Telescopes with >30 m diameters will require WFS detectors that have large pixel formats (512x512), low noise (<3 e-/pixel) and very high frame rates (~1 kHz). These requirements have led to the idea of a bare CMOS active pixel device (the Medipix2 chip) functioning in counting mode as an anode with noiseless readout for a microchannel plate (MCP) detector and at 1 kHz continuous frame rate. First measurement results obtained with this novel detector are presented both for UV photons and beta particles.

  7. Reference analysis of the signal + background model in counting experiments

    Science.gov (United States)

    Casadei, D.

    2012-01-01

    The model representing two independent Poisson processes, labelled as ``signal'' and ``background'' and both contributing additively to the total number of counted events, is considered from a Bayesian point of view. This is a widely used model for the searches of rare or exotic events in presence of a background source, as for example in the searches performed by high-energy physics experiments. In the assumption of prior knowledge about the background yield, a reference prior is obtained for the signal alone and its properties are studied. Finally, the properties of the full solution, the marginal reference posterior, are illustrated with few examples.

  8. Buckling Analysis of Unidirectional PolymerMatrix Composite Plates

    Directory of Open Access Journals (Sweden)

    Jawad Kadhim Uleiwi

    2006-01-01

    Full Text Available This study deals with the estimation of critical load of unidirectional polymer matrix composite plates by using experimental and finite element techniques at different fiber angles and fiber volume fraction of the composite plate.Buckling analysis illustrated that the critical load decreases in nonlinear relationship with the increase of the fiber angle and that it increases with the increase of the fiber volume fraction.The results show that the maximum value of the critical load is (629.54 N/m at (? = 0? and (Vf = 40 % for the finite element method, while the minimum value of the critical load is (49 N/m at (? = 90? and (Vf = 10 % for the experimental results. The results also indicated that the maximum difference between the finite element analysis and experimental work is about (11 % at ( ? = 0? and (Vf = 40 %

  9. Stochastic Finite Element Analysis of Plate and Shell Construction

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The response of random plate and shell construction is analyzed with the stochastic finite element method (SFEM). Random material properties and geometric dimensions of construction are involved in this paper. A simplified isoparametric local average model is used to describe the random field. Numerical results of the examples indicate that the approach presented herein is an economical and efficient solution for such an analysis compared with Monte Carlo simulation (MCS).

  10. Analysis of Interplanetary Dust Experiment Detectors and Other Witness Plates

    Science.gov (United States)

    Griffis, D. P.; Wortman, J. J.

    1992-01-01

    The development of analytical procedures for identifying the chemical composition of residue from impacts that occurred on the Interplanetary Dust Experiment (IDE) detectors during the flight of Long Duration Exposure Facility (LDEF) and the carrying out of actual analysis on IDE detectors and other witness plates are discussed. Two papers on the following topics are presented: (1) experimental analysis of hypervelocity microparticle impact sites on IDE sensor surfaces; and (2) contaminant interfaces with secondary Ion Mass Spectrometer (SIMS) analysis of microparticle impactor residues on LDEF surfaces.

  11. Cross strip anode readouts for large format, photon counting microchannel plate detectors: developing flight qualified prototypes of the detector and electronics

    Science.gov (United States)

    Vallerga, John; Raffanti, Rick; Cooney, Michael; Cumming, Harley; Varner, Gary; Seljak, Andrej

    2014-07-01

    Photon counting microchannel plate (MCP) imagers have been the detector of choice for most UV astronomical missions over the last two decades (e.g. EUVE, FUSE, COS on Hubble etc.). Over this duration, improvements in the MCP laboratory readout technology have resulted in better spatial resolution (x10), temporal resolution (x1000) and output event rate (x100), all the while operating at lower gain (x 10) resulting in lower high voltage requirements and longer MCP lifetimes. One such technology is the parallel cross strip (PXS) readout. Laboratory versions of PXS electronics have demonstrated count rates on the order of 2 MHz, and temporal resolution of ~ 1ns. In 2012 our group at U.C. Berkeley, along with our partners at the U. Hawaii, received a Strategic Astrophysics Technology grant to raise the TRL of the PXS detector and electronics from 4 to 6 by replacing most of the high powered electronics with application specific integrated circuits (ASICs) which will lower the power, mass and volume requirements of the PXS detector. We were also tasked to design and fabricate a "standard" 50mm square active area MCP detector incorporating these electronics that can be environmentally qualified for flight (temperature, vacuum, vibration). The first ASICs designed for this program have been fabricated and are undergoing testing. We present the latest progress on these ASIC designs and performance and show imaging results from the new 50 x 50 mm XS detector.

  12. Studies on the ecology of aquatic bacteria of the lower Niger Delta: multiple antibiotic resistance among the standard plate count organisms.

    Science.gov (United States)

    Ogan, M T; Nwiika, D E

    1993-05-01

    The ecology of multiple antibiotic resistant (MAR) bacteria in the fresh-waters of the lower Niger Delta was studied in the Port Harcourt area, Rivers State. On the basis of decreasing pollution levels three zones, A, B, C, were recognized. Cell recovery by two viable count media, casein-peptone-starch (CPS) and plate count (PC) agar containing chloramphenicol, tetracycline, penicillin, streptomycin or ampicillin were compared in an initial study. Higher numbers of antibiotic resistant (AR) bacteria were recovered on CPS containing tetracycline, penicillin, streptomycin and ampicillin from the faecally-polluted New Calabar River (zone A) than on SPC agar containing similar antibiotics but the reverse was observed for forest stream (zone B) samples. Differences between the two media were also observed at individual sample sites. The proportions of strains of AR bacteria resistant to their primary isolation antibiotic varied from 55% (zone B) to 72% in the least polluted Isiokpo and Elele-Alimini streams (zone C), for ampicillin, and mostly count media without antibiotics included mainly species of Bacillus (12) and enterobacteria (18). Between five and 10 strains were resistant to > or = three antibiotics; seven were resistant to all five. The antibiograms of most strains were variable and depended on the method of drug application (discs or incorporation into agar), media and temperature of incubation (25 degrees, 37 degrees or 44.5 degrees C). Twenty-one strains were consistently resistant to ampicillin by the two methods; 10 to 19 were consistent for chloramphenicol, tetracycline and penicillin.(ABSTRACT TRUNCATED AT 250 WORDS)

  13. Identification and characterization of elevated microbial counts in bulk tank raw milk.

    Science.gov (United States)

    Hayes, M C; Ralyea, R D; Murphy, S C; Carey, N R; Scarlett, J M; Boor, K J

    2001-01-01

    The bacterial composition of bulk tank milk from 13 farms was examined over a 2-wk period to characterize sudden elevations in the total bacterial count referred to as "spikes." Bulk tank milk samples collected at each pick-up were analyzed for standard plate count, Petrifilm aerobic count, somatic cell count, gram-negative organisms, and streptococci. Twenty standard plate count spikes were observed: 12 associated with streptococci, 4 associated with gram-negative organisms, 2 associated with streptococci and gram-negative organisms, and 2 that were not definitively characterized. Spikes ranged from 14,000 to 600,000 cfu/ml. Streptococcus uberis was isolated as the predominant organism from 11 spikes, and Escherichia coli was isolated from 4 spikes. Statistical analysis of total bacterial counts indicated a high correlation (r = 0.94) between standard plate counts and Petrifilm aerobic count. Regression analysis of standard plate counts and Petrifilm aerobic counts yielded the equation log10 (standard plate count) = 0.73 + 0.85log10 (Petrifilm aerobic count), indicating that the correlation, although strong, is not one to one. In a related pilot study, triplicate bulk tank milk samples were collected and analyzed for total bacterial count and presumptive streptococcus, gram-negative, and staphylococcus counts. Two-way ANOVA of these triplicate data indicated a lack of significant variation among the triplicate samples, suggesting that one sample can reliably gauge the microbial status of the entire bulk tank.

  14. Changes in Aerobic Plate and Escherichia coli-Coliform Counts and in Populations of Inoculated Foodborne Pathogens on Inshell Walnuts during Storage.

    Science.gov (United States)

    Frelka, John C; Davidson, Gordon R; Harris, Linda J

    2016-07-01

    After harvest, inshell walnuts are dried using low-temperature forced air and are then stored in bins or silos for up to 1 year. To better understand the survival of bacteria on inshell walnuts, aerobic plate counts (APCs) and Escherichia coli?coliform counts (ECCs) were evaluated during commercial storage (10 to 12°C and 63 to 65% relative humidity) over 9 months. APCs decreased by 1.4 to 2.0 log CFU per nut during the first 5 months of storage, and ECCs decreased by 1.3 to 2.2 log CFU per nut in the first month of storage. Through the remaining 4 to 8 months of storage, APCs and ECCs remained unchanged (P > 0.05) or decreased by CFU per nut per month. Similar trends were observed on kernels extracted from the inshell walnuts. APCs and ECCs were consistently and often significantly higher on kernels extracted from visibly broken inshell walnuts than on kernels extracted from visibly intact inshell walnuts. Parameters measured in this study were used to determine the survival of five-strain cocktails of E. coli O157:H7, Listeria monocytogenes, and Salmonella inoculated onto freshly hulled inshell walnuts (∼8 log CFU/g) after simulated commercial drying (10 to 12 h; 40°C) and simulated commercial storage (12 months at 10°C and 65% relative humidity). Populations declined by 2.86, 5.01, and 4.40 log CFU per nut for E. coli O157:H7, L. monocytogenes, and Salmonella, respectively, after drying and during the first 8 days of storage. Salmonella populations changed at a rate of -0.33 log CFU per nut per month between days 8 and 360, to final levels of 2.83 ± 0.79 log CFU per nut. E. coli and L. monocytogenes populations changed by -0.17 log CFU per nut per month and -0.26 log CFU per nut per month between days 8 and 360, respectively. For some samples, E. coli or L. monocytogenes populations were below the limit of detection by plating (0.60 log CFU per nut) by day 183 or 148, respectively; at least one of the six samples was positive at each subsequent sampling

  15. X-ray photon-counting detector based on a micro-channel plate for pulsar navigation

    Institute of Scientific and Technical Information of China (English)

    Baomei Chen; Baosheng Zhao; Huijun Hu; Qiurong Yan; Lizhi Sheng

    2011-01-01

    The pulse time of arrival (TOA) is a determining parameter for accurate timing and positioning in X-ray pulsar navigation. The pulse TOA can be calculated by comparing the measured arrival time with the predicted arrival time of the X-ray pulse for pulsar. In this study, in order to research the measurement of pulse arrival time, an experimental system is set up. The experimental system comprises a simulator of the X-ray pulsar, an X-ray detector, a time-measurement system, and a data-processing system. An X-ray detector base is proposed on the basis of the micro-channel plate (MCP), which is sensitive to soft X-ray in the 1-10 keV band. The MCP-based detector, the structure and principle of the experimental system, and results of the pulse profile are described in detail. In addition, a discussion of the effects of different X-ray pulse periods and the quantum efficiency of the detector on pulse-profile signal-to-noise ratio (SNR) is presented. Experimental results reveal that the SNR of the measured pulse profile becomes enhanced as the quantum efficiency of the detector increases. The SNR of the pulse profile is higher when the period of the pulse is smaller at the same integral.%@@ The pulse time of arrival (TOA) is a determining parameter for accurate timing and positioning in X-ray pulsar navigation.The pulse TOA can be calculated by comparing the measured arrival time with the predicted arrival time of the X-ray pulse for pulsar.In this study, in order to research the measurement of pulse arrival time, an experimental system is set up.The experimental system comprises a simulator of the X-ray pulsar, an X-ray detector, a time-measurement system, and a data-processing system.An X-ray detector base is proposed on the basis of the micro-channel plate (MCP), which is sensitive to soft X-ray in the 1-10 keV band.The MCP-based detector, the structure and principle of the experimental system,and results of the pulse profile are described in detail.In addition, a

  16. Modal analysis of two identical circular plates coupled with fluid

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Kyeong Hoon; Lee, Gyu Mahn; Park, Keun Bae; Chang, Moon Hee

    2001-01-01

    An analytical method for evaluating the free vibration of two identical circular plates coupled with fluid was developed by assuming the clamped boundary condition of the plates and an ideal fluid. The method was based upon the finite Fourier-Bessel series expansion and the Rayleigh-Riz method. The proposed method is verified by finite element analyses using commercial software, ANSYS (release 5.6) with good accuracy. Two transverse vibration modes, namely in-phase and out-of-phase, are observed alternately in the fluid-coupled system when the number of nodal circles increases for the fixed nodal diameter. It is found that the normalized natural frequency of the fluid-coupled system monotonically increases with an increase in the number of nodal diameters and circles by virtue of a decrease in relative hydrodynamic mass. The effect of distance between the circular plates and the effect of fluid bounding on the fluid-coupled natural frequencies are also investigated. The theory developed in this report can be applicable to the dynamic analysis of a bottom screen assembly in SMART integral reactor.

  17. Correlation analysis on total lymphocyte count and CD4 count in HIV-infected patients: a retrospective evaluation.

    Science.gov (United States)

    Wang, Yuming; Liang, Shuying; Yu, Erman; Guo, Jinling; Li, Zizhao; Wang, Zhe; Du, Yukai

    2011-10-01

    CD4 count is the standard method for determining eligibility for highly active antiretroviral therapy (HAART) and monitoring HIV/AIDS disease progression, but it is not widely available in resource-limited settings. This study examined the correlation between total lymphocyte count (TLC) and CD4 count of HIV-infected patients before and after HAART, and assessed the thresholds of TLC for making decisions about the initiation and for monitoring HAART. A retrospective study was performed, and 665 HIV-infected patients with TLC and CD4 count from four counties (Shangcai, Queshan, Shenqiu and Weishi) were included in the study. Pearson correlation and receiver operating characteristic (ROC) were used. TLC and CD4 count after HAART was significantly increased as compared with pre-HAART (PHIV-infected individuals for making decisions about the initiation and for monitoring HAART in resource-limited settings.

  18. Cooling a solar telescope enclosure: plate coil thermal analysis

    Science.gov (United States)

    Gorman, Michael; Galapon, Chriselle; Montijo, Guillermo; Phelps, LeEllen; Murga, Gaizka

    2016-08-01

    provides a high degree of confidence for the performance of the full system when it is installed. If time allows, additional testing could be done that includes additional incident angles and times of day. This would allow a more complete analysis. If additional testing were to be performed, it's recommended to use a larger chiller capable of reaching lower temperatures. The test rig design could also be optimized in order to bring the plate coil up to its maximum efficiency. In the future, the script could be rewritten in a different computer language, so that the data could be solved for quicker. Further analysis could also include different types of coolants.

  19. UPDATE ON MECHANICAL ANALYSIS OF MONOLITHIC FUEL PLATES

    Energy Technology Data Exchange (ETDEWEB)

    D. E. Burkes; F. J. Rice; J.-F. Jue; N. P. Hallinan

    2008-03-01

    Results on the relative bond strength of the fuel-clad interface in monolithic fuel plates have been presented at previous RRFM conferences. An understanding of mechanical properties of the fuel, cladding, and fuel / cladding interface has been identified as an important area of investigation and quantification for qualification of monolithic fuel forms. Significant progress has been made in the area of mechanical analysis of the monolithic fuel plates, including mechanical property determination of fuel foils, cladding processed by both hot isostatic pressing and friction bonding, and the fuel-clad composite. In addition, mechanical analysis of fabrication induced residual stress has been initiated, along with a study to address how such stress can be relieved prior to irradiation. Results of destructive examinations and mechanical tests are presented along with analysis and supporting conclusions. A brief discussion of alternative non-destructive evaluation techniques to quantify not only bond quality, but also bond integrity and strength, will also be provided. These are all necessary steps to link out-of-pile observations as a function of fabrication with in-pile behaviours.

  20. Comparison of the compact dry TC method with the standard pour plate method (AOAC official method 966.23) for determining aerobic colony counts in food samples: Performance-tested method.

    Science.gov (United States)

    Kodaka, Hidemasa; Mizuochi, Shingo; Teramura, Hajime; Nirazuka, Tadanobu

    2005-01-01

    Compact Dry TC qualifies as a rapid method kit for determining aerobic colony counts in foods. The plates are presterilized and contain culture medium and a cold-soluble gelling agent. The medium is rehydrated by inoculating 1 mL diluted sample into the center of the self-diffusible medium and allowing the solution to diffuse by capillary action. The plates can then be incubated and the colonies counted without any additional steps. The Compact Dry TC method was validated with 5 different raw meats. The performance tests were conducted at 35 degrees and 30 degrees C. In all required performance studies, no apparent differences were observed between the Compact Dry TC method and the Standard Pour Plate method (AOAC Official Method 966.23) for the detection level of aerobic microorganisms. For the accuracy claim (n = 60), a correlation factor of r2(35) = 0.9977 (35 degrees C) and r2(30) = 0.9932 (30 degrees C) could be assigned, as stated in the application for "Performance Tested Method." Quality consistency and storage robustness studies, showed no significant variations in plate count results with different production lots or plates of diverse storage age.

  1. A Bayesian Semi-parametric Approach for the Differential Analysis of Sequence Counts Data.

    Science.gov (United States)

    Guindani, Michele; Sepúlveda, Nuno; Paulino, Carlos Daniel; Müller, Peter

    2014-04-01

    Data obtained using modern sequencing technologies are often summarized by recording the frequencies of observed sequences. Examples include the analysis of T cell counts in immunological research and studies of gene expression based on counts of RNA fragments. In both cases the items being counted are sequences, of proteins and base pairs, respectively. The resulting sequence-abundance distribution is usually characterized by overdispersion. We propose a Bayesian semi-parametric approach to implement inference for such data. Besides modeling the overdispersion, the approach takes also into account two related sources of bias that are usually associated with sequence counts data: some sequence types may not be recorded during the experiment and the total count may differ from one experiment to another. We illustrate our methodology with two data sets, one regarding the analysis of CD4+ T cell counts in healthy and diabetic mice and another data set concerning the comparison of mRNA fragments recorded in a Serial Analysis of Gene Expression (SAGE) experiment with gastrointestinal tissue of healthy and cancer patients.

  2. Analysing count data of Butterflies communities in Jasin, Melaka: A Poisson regression analysis

    Science.gov (United States)

    Afiqah Muhamad Jamil, Siti; Asrul Affendi Abdullah, M.; Kek, Sie Long; Nor, Maria Elena; Mohamed, Maryati; Ismail, Norradihah

    2017-09-01

    Counting outcomes normally have remaining values highly skewed toward the right as they are often characterized by large values of zeros. The data of butterfly communities, had been taken from Jasin, Melaka and consists of 131 number of subject visits in Jasin, Melaka. In this paper, considering the count data of butterfly communities, an analysis is considered Poisson regression analysis as it is assumed to be an alternative way on better suited to the counting process. This research paper is about analysing count data from zero observation ecological inference of butterfly communities in Jasin, Melaka by using Poisson regression analysis. The software for Poisson regression is readily available and it is becoming more widely used in many field of research and the data was analysed by using SAS software. The purpose of analysis comprised the framework of identifying the concerns. Besides, by using Poisson regression analysis, the study determines the fitness of data for accessing the reliability on using the count data. The finding indicates that the highest and lowest number of subject comes from the third family (Nymphalidae) family and fifth (Hesperidae) family and the Poisson distribution seems to fit the zero values.

  3. Statistical analysis of dark count rate in Geiger-mode APD FPAs

    Science.gov (United States)

    Itzler, Mark A.; Krishnamachari, Uppili; Chau, Quan; Jiang, Xudong; Entwistle, Mark; Owens, Mark; Slomkowski, Krystyna

    2014-10-01

    We present a temporal statistical analysis of the array-level dark count behavior of Geiger-mode avalanche photodiode (GmAPD) focal plane arrays that distinguishes between Poissonian intrinsic dark count rate and non-Poissonian crosstalk counts by considering "inter-arrival" times between successive counts from the entire array. For 32 x 32 format sensors with 100 μm pixel pitch, we show the reduction of crosstalk for smaller active area sizes within the pixel. We also compare the inter-arrival time behavior for arrays with narrow band (900 - 1100 nm) and broad band (900 - 1600 nm) spectral response. We then consider a similar analysis of larger format 128 x 32 arrays. As a complement to the temporal analysis, we describe the results of a spatial analysis of crosstalk events. Finally, we propose a simple model for the impact of crosstalk events on the Poissonian statistics of intrinsic dark counts that provides a qualitative explanation for the results of the inter-arrival time analysis for arrays with varying degrees of crosstalk.

  4. An analysis of dependency of counting efficiency on worker anatomy for in vivo measurements: whole-body counting

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Binquan; Mille, Matthew; Xu, X George [Nuclear Engineering and Engineering Physics, Rensselaer Polytechnic Institute, Troy, NY 12180 (United States)], E-mail: xug2@rpi.edu

    2008-07-07

    In vivo radiobioassay is integral to many health physics and radiological protection programs dealing with internal exposures. The Bottle Manikin Absorber (BOMAB) physical phantom has been widely used for whole-body counting calibrations. However, the shape of BOMAB phantoms-a collection of plastic, cylindrical shells which contain no bones or internal organs-does not represent realistic human anatomy. Furthermore, workers who come in contact with radioactive materials have rather different body shape and size. To date, there is a lack of understanding about how the counting efficiency would change when the calibrated counter is applied to a worker with complicated internal organs or tissues. This paper presents a study on various in vivo counting efficiencies obtained from Monte Carlo simulations of two BOMAB phantoms and three tomographic image-based models (VIP-Man, NORMAN and CNMAN) for a scenario involving homogeneous whole-body radioactivity contamination. The results reveal that a phantom's counting efficiency is strongly dependent on the shape and size of a phantom. Contrary to what was expected, it was found that only small differences in efficiency were observed when the density and material composition of all internal organs and tissues of the tomographic phantoms were changed to water. The results of this study indicate that BOMAB phantoms with appropriately adjusted size and shape can be sufficient for whole-body counting calibrations when the internal contamination is homogeneous.

  5. An analysis of dependency of counting efficiency on worker anatomy for in vivo measurements: whole-body counting.

    Science.gov (United States)

    Zhang, Binquan; Mille, Matthew; Xu, X George

    2008-07-07

    In vivo radiobioassay is integral to many health physics and radiological protection programs dealing with internal exposures. The Bottle Manikin Absorber (BOMAB) physical phantom has been widely used for whole-body counting calibrations. However, the shape of BOMAB phantoms-a collection of plastic, cylindrical shells which contain no bones or internal organs-does not represent realistic human anatomy. Furthermore, workers who come in contact with radioactive materials have rather different body shape and size. To date, there is a lack of understanding about how the counting efficiency would change when the calibrated counter is applied to a worker with complicated internal organs or tissues. This paper presents a study on various in vivo counting efficiencies obtained from Monte Carlo simulations of two BOMAB phantoms and three tomographic image-based models (VIP-Man, NORMAN and CNMAN) for a scenario involving homogeneous whole-body radioactivity contamination. The results reveal that a phantom's counting efficiency is strongly dependent on the shape and size of a phantom. Contrary to what was expected, it was found that only small differences in efficiency were observed when the density and material composition of all internal organs and tissues of the tomographic phantoms were changed to water. The results of this study indicate that BOMAB phantoms with appropriately adjusted size and shape can be sufficient for whole-body counting calibrations when the internal contamination is homogeneous.

  6. Non-linear analysis of vibrations of irregular plates

    Science.gov (United States)

    Lobitz, D. W.; Nayfeh, A. H.; Mook, D. T.

    1977-01-01

    A numerical perturbation method is used to investigate the forced vibrations of irregular plates. Nonlinear terms associated with the midplane stretching are retained in the analysis. The numerical part of the method involves the use of linear, finite element techniques to determine the free oscillation mode shapes and frequencies and to obtain the linear midplane stress resultants caused by the midplane stretching. Representing the solution as an expansion in terms of these linear mode shapes, these modes and the resultants are used to determine the equations governing the time-dependent coefficients of this expansion. These equations are solved by using the method of multiple scales. Specific solutions are given for the main-resonant vibrations of an elliptical plate in the presence of internal resonances. The results indicate that modes other than the driven mode can be drawn into the steady state response. Though the excitation is composed of a single harmonic, the response may not be periodic. Moreover, the particular types of responses that can occur are highly dependent on the mode being excited and are sensitive to small geometrical changes.

  7. NUMERICAL ANALYSIS OF DELAMINATION GROWTH FOR STIFFENED COMPOSITE LAMINATED PLATES

    Institute of Scientific and Technical Information of China (English)

    白瑞祥; 陈浩然

    2004-01-01

    A study of postbuckling and delamination propagation behavior in delaminated stiffened composite plates was presented. A methodology was proposed for simulating the multi-failure responses, such as initial and postbuckling, delamination onset and propagation, etc. A finite element analysis was conducted on the basis of the Mindlin first order shear effect theory and the von-Krmn nonlinear deformation assumption. The total energy release rate used as the criteria of delamination growth was estimated with virtual crack closure technique (VCCT). A self-adaptive grid moving technology was adopted to model the delamination growth process. Moreover, the contact effect along delamination front was also considered during the numerical simulation process. By some numerical examples, the influence of distribution and location of stiffener, configuration and size of the delamination, boundary condition and contact effect upon the delamination growth behavior of the stiffened composite plates were investigated. The method and numerical conclusion provided should be of great value to engineers dealing with composite structures.

  8. How many fish in a tank? Constructing an automated fish counting system by using PTV analysis

    Science.gov (United States)

    Abe, S.; Takagi, T.; Takehara, K.; Kimura, N.; Hiraishi, T.; Komeyama, K.; Torisawa, S.; Asaumi, S.

    2017-02-01

    Because escape from a net cage and mortality are constant problems in fish farming, health control and management of facilities are important in aquaculture. In particular, the development of an accurate fish counting system has been strongly desired for the Pacific Bluefin tuna farming industry owing to the high market value of these fish. The current fish counting method, which involves human counting, results in poor accuracy; moreover, the method is cumbersome because the aquaculture net cage is so large that fish can only be counted when they move to another net cage. Therefore, we have developed an automated fish counting system by applying particle tracking velocimetry (PTV) analysis to a shoal of swimming fish inside a net cage. In essence, we treated the swimming fish as tracer particles and estimated the number of fish by analyzing the corresponding motion vectors. The proposed fish counting system comprises two main components: image processing and motion analysis, where the image-processing component abstracts the foreground and the motion analysis component traces the individual's motion. In this study, we developed a Region Extraction and Centroid Computation (RECC) method and a Kalman filter and Chi-square (KC) test for the two main components. To evaluate the efficiency of our method, we constructed a closed system, placed an underwater video camera with a spherical curved lens at the bottom of the tank, and recorded a 360° view of a swimming school of Japanese rice fish (Oryzias latipes). Our study showed that almost all fish could be abstracted by the RECC method and the motion vectors could be calculated by the KC test. The recognition rate was approximately 90% when more than 180 individuals were observed within the frame of the video camera. These results suggest that the presented method has potential application as a fish counting system for industrial aquaculture.

  9. Introduction to Analysis and Design of Plate Panels

    DEFF Research Database (Denmark)

    Jensen, Jørgen Juncher; Lützen, Marie

    The present notes cover plate theory dealing with bending, vibrations, elastic buckling and ultimate strength. The plate structures considered are isotropic, orthotropic and stiffened plates made of metallic materials. The main objective of the notes is to give an introduction to plates and plate....... Comments and amendments received by the students in the course have had a significant influence on the present layout. A special thanks to Torben Christiansen for careful proof-readings of the examples and valuable improvements.......¨utzen in 2002. It has now been amended and extended with ultimate strength of plates, an introduction to the theory of shells and additional examples to cover the lecture material for the course ”41215 Plate and Shell Structures” at the Department of Mechanical Engineering, the Technical University of Denmark...

  10. Bending and buckling behavior analysis of foamed metal circular plate.

    Science.gov (United States)

    Fan, Jian Ling; Ma, Lian Sheng; Zhang, Lu; De Su, Hou

    2016-07-04

    This paper establishes a density gradient model along the thickness direction of a circular plate made of foamed material. Based on the first shear deformation plate theory, the result is deduced that the foamed metal circular plate with graded density along thickness direction yields axisymmetric bending problem under the action of uniformly distributed load, and the analytical solution is obtained by solving the governing equation directly. The analyses on two constraint conditions of edge radial clamping and simply supported show that the density gradient index and external load may affect the axisymmetric bending behavior of the plate. Then, based on the classical plate theory, the paper analyzes the behavior of axisymmetric buckling under radial pressure applied on the circular plate. Shooting method is used to obtain the critical load, and the effects of gradient nature of material properties and boundary conditions on the critical load of the plate are analyzed.

  11. Linear and nonlinear buckling analysis of a locally stretched plate

    Energy Technology Data Exchange (ETDEWEB)

    Kilardj, Madina; Ikhenzzen, Ghania [University of Sciences and Technology Houari Boumediene (U.S.T.H.B), Bab Ezzouar, Algiers (Algeria); Merssager, Tanguy; Kanit, Toufik [Laboratoire de Mecanique de Lille Universite Lille 1, Cite ScientifiqueVilleneuve d' Ascq cedex (France)

    2016-08-15

    Uniformly stretched thin plates do not buckle unless they are in special boundary conditions. However, buckling commonly occurs around discontinuities, such as cracks, cuts, narrow slits, holes, and different openings, of such plates. This study aims to show that buckling can also occur in thin plates that contain no defect or singularity when the stretching is local. This specific stability problem is analyzed with the finite element method. A brief literature review on stretched plates is presented. Linear and nonlinear buckling stress analyses are conducted for a partially stretched rectangular plate, and various load cases are considered to investigate the influence of the partial loading expanse on the critical tensile buckling load. Results are summarized in iso-stress areas, tables and graphs. Local stretching on one end of the plate induces buckling in the thin plate even without geometrical imperfection.

  12. Comparison of the compact dry TC and 3M petrifilm ACP dry sheet media methods with the spiral plate method for the examination of randomly selected foods for obtaining aerobic colony counts.

    Science.gov (United States)

    Ellis, P; Meldrum, R

    2002-02-01

    Two hundred thirty-six randomly selected food and milk samples were examined to obtain aerobic colony counts by two dry sheet media methods and a standard Public Health Laboratory Service spiral plate method. Results for 40 samples were outside the limits of detection for one or more of the tested methods and were not considered. (The limits of detection for the spiral plate method were 200 to 1 x 10(8) CFU/ml for the spiral plate method and 100 to 3 x 10(6) CFU/ml for the dry sheet media methods.) The remaining 196 sets of results were analyzed further. When the results from the three methods were compared, correlation coefficients were all >0.80 and slopes and intercepts were close to 1.0 and 0.0, respectively. Mean log values and standard deviations were very similar for all three methods. The results were evaluated according to published UK guidelines for ready-to-eat foods sampled at the point of sale, which include a quality acceptability assessment that is based on aerobic colony counts. Eighty-six percent of the comparable results gave the same verdict with regard to acceptability according to the aerobic colony count guidelines. Both dry sheet media methods were comparable to the spiral plate method and can be recommended for the examination of food.

  13. Uncertainty evaluation of aerobic plate count by proficiency testing%能力验证菌落总数测定结果不确定度的评定

    Institute of Scientific and Technical Information of China (English)

    王海华; 兰茜

    2015-01-01

    目的:对实验室能力验证样品菌落总数进行不确定度评定。方法能力验证菌落总数依据 GB 4789.2-2010《食品安全国家标准食品微生物学检验菌落总数测定》以及能力验证计划参试指导书来进行测定和结果判断,再根据JJF 1059.1-2012《测量不确定度评定与表示》的规定,对测定过程中引入的不确定度分量进行评定,然后采用合成的方法来计算和评定菌落总数的不确定度。结果菌落总数测定结果合成不确定度为0.05042,扩展不确定度为0.1008(以对数计)。结论本研究建立的方法可以对能力验证样品的菌落总数进行不确定度评定,此方法适合于类似检测条件下菌落总数不确定度的评定。%ABSTRACT:Objective To evaluate the uncertainty of aerobic plate count by proficiency testing. Methods Proficiency testing of aerobic plate count was conducted under the direction of GB 4789.2-2010 National food safety standard microbiological examination in food:Aerobic plate count and CFAPA-024 Reference book for microbiological ability verification plan in food. Then, the uncertainty components introduced in the experiment were determined according to JJF1059.1-2012 Evaluation and expression of uncertainty in measurements. Finally, synthetic method was used to calculate and assess the uncertainty of aerobic plate count. Results The combined uncertainty of aerobic plate count was 0.05042, the expanded uncertainty was U(1og)=0.1008. Conclusion The method established in this study can be used in the uncertainty evaluation of aerobic plate count under similar circumstances.

  14. Correlations between total cell concentration, total adenosine tri-phosphate concentration and heterotrophic plate counts during microbial monitoring of drinking water

    Directory of Open Access Journals (Sweden)

    F. Hammes

    2008-06-01

    Full Text Available The general microbial quality of drinking water is normally monitored by heterotrophic plate counts (HPC. This method has been used for more than 100 years and is recommended in drinking water guidelines. However, the HPC method is handicapped because it is time-consuming and restricted to culturable bacteria. Recently, rapid and accurate detection methods have emerged, such as adenosine tri-phosphate (ATP measurements to assess microbial activity in drinking water, and flow cytometry (FCM to determine the total cell concentration (TCC. It is necessary and important for drinking water quality control to understand the relationships among the conventional and new methods. In the current study, all three methods were applied to 200 drinking water samples obtained from two local buildings connected to the same distribution system. Samples were taken both on normal working days and weekends, and the correlations between the different microbiological parameters were determined. TCC in the samples ranged from 0.37–5.61×105 cells/ml, and two clusters, the so-called high (HNA and low (LNA nucleic acid bacterial groups, were clearly distinguished. The results showed that the rapid determination methods (i.e., FCM and ATP correlated well (R2=0.69, but only a weak correlation (R2=0.31 was observed between the rapid methods and conventional HPC data. With respect to drinking water monitoring, both FCM and ATP measurements were confirmed to be useful and complimentary parameters for rapid assessing of drinking water microbial quality.

  15. Evaluation of the limulus amoebocyte lysate test in conjunction with a gram negative bacterial plate count for detecting irradiation of chicken

    Science.gov (United States)

    Scotter, Susan L.; Wood, Roger; McWeeny, David J.

    A study to evaluate the potential of the Limulus amoebocyte lysate (LAL) test in conjuction with a Gram negative bacteria (GNB) plate count for detecting the irradiation of chicken is described. Preliminary studies demonstrated that chickens irradiated at an absorbed dose of 2.5 kGy could be differentiated from unirradiated birds by measuring levels of endotoxin and of numbers of GNB on chicken skin. Irradiated birds were found to have endotoxin levels similar to those found in unirradiated birds but significantly lower numbers of GNB. In a limited study the test was found to be applicable to birds from different processors. The effect of temperature abuse on the microbiological profile, and thus the efficacy of the test, was also investigated. After temperature abuse, the irradiated birds were identifiable at worst up to 3 days after irradiation treatment at the 2.5 kGy level and at best some 13 days after irradiation. Temperature abuse at 15°C resulted in rapid recovery of surviving micro-organisms which made differentiation of irradiated and unirradiated birds using this test unreliable. The microbiological quality of the bird prior to irradiation treatment also affected the test as large numbers of GNB present on the bird prior to irradiation treatment resulted in larger numbers of survivors. In addition, monitoring the developing flora after irradiation treatment and during subsequent chilled storage also aided differentiation of irradiated and unirradiated birds. Large numbers of yeasts and Gram positive cocci were isolated from irradiated carcasses whereas Gram negative oxidative rods were the predominant spoilage flora on unirradiated birds.

  16. Calibration and equivalency analysis of image plate scanners

    Energy Technology Data Exchange (ETDEWEB)

    Williams, G. Jackson, E-mail: williams270@llnl.gov; Maddox, Brian R.; Chen, Hui [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550 (United States); Kojima, Sadaoki [Institute of Laser Engineering, Osaka University, Yamada-oka, 2-6, Suita, Osaka 565-0871 (Japan); Millecchia, Matthew [Laboratory for Laser Energetics, University of Rochester, 250 East River Road, Rochester, New York 14623 (United States)

    2014-11-15

    A universal procedure was developed to calibrate image plate scanners using radioisotope sources. Techniques to calibrate scanners and sources, as well as cross-calibrate scanner models, are described to convert image plate dosage into physical units. This allows for the direct comparison of quantitative data between any facility and scanner. An empirical relation was also derived to establish sensitivity response settings for arbitrary gain settings. In practice, these methods may be extended to any image plate scanning system.

  17. Dynamic Analysis of Modifications to Simple Plate Tectonic Theory

    Science.gov (United States)

    Paczkowski, Karen

    A number of geological and geophysical observations suggest significant departures from simple, first-order plate tectonic theory. In this thesis we address the dynamic implications of some of these observations and propose generalized theories to explain their dynamics and conditions of formation. In Chapter 2, we develop a generalized theory and analytic model to predict the conditions under which large-volume removal of continental lithosphere can occur through the formation of drip instabilities. Using damage physics relevant for Earth, we find a large portion of the lithosphere may be mobilized and entrained into growing drip instabilities. For a critical amount of damage, the growth is accelerated sufficiently that large-volume drip instabilities may form within geologically feasible time frames. Our model suggests large-volume lithospheric drip instabilities may arise independently of tectonic settings through damage-assisted mobilization and entrainment of the highly viscous lithosphere. In Chapter 3, we develop a mechanical model independent of volcanism and thermal weakening to explain the initial formation and length scale of rifting and extension near convergent plate boundaries. We conduct a linear stability analysis of a simple viscous necking model, which includes the lithosphere's negative buoyancy, non-Newtonian rheology, and freely moving top surface, to determine which properties of the lithosphere govern the location of rifting. We find that the negative buoyancy of the lithosphere promotes the formation of rifting structures when simple Newtonian viscosities are present. However, localized weakening, introduced through a power law exponent, is required to generate realistic rifting length scales. Our model suggests that the initial location of rifting in the overriding plate at subduction zones is primarily due to the mechanical extension induced by rollback of the subducting slab. In Chapter 4, we propose a theory to explain the seismic

  18. Guidelines for monitoring bulk tank milk somatic cell and bacterial counts.

    Science.gov (United States)

    Jayarao, B M; Pillai, S R; Sawant, A A; Wolfgang, D R; Hegde, N V

    2004-10-01

    This study was conducted to establish guidelines for monitoring bulk tank milk somatic cell count and bacterial counts, and to understand the relationship between different bacterial groups that occur in bulk tank milk. One hundred twenty-six dairy farms in 14 counties of Pennsylvania participated, each providing one bulk tank milk sample every 15 d for 2 mo. The 4 bulk tank milk samples from each farm were examined for bulk tank somatic cell count and bacterial counts including standard plate count, preliminary incubation count, laboratory pasteurization count, coagulase-negative staphylococcal count, environmental streptococcal count, coliform count, and gram-negative noncoliform count. The milk samples were also examined for presence of Staphylococcus aureus, Streptococcus agalactiae, and Mycoplasma. The bacterial counts of 4 bulk tank milk samples examined over an 8-wk period were averaged and expressed as mean bacterial count per milliliter. The study revealed that an increase in the frequency of isolation of Staphylococcus aureus and Streptococcus agalactiae was significantly associated with an increased bulk tank somatic cell count. Paired correlation analysis showed that there was low correlation between different bacterial counts. Bulk tank milk with low (standard plate count also had a significantly low level of mean bulk tank somatic cell count (count (count (counts (count (count was less likely to be associated with somatic cell or other bacterial counts. Herd size and farm management practices had considerable influence on somatic cell and bacterial counts in bulk tank milk. Dairy herds that used automatic milking detachers, sand as bedding material, dip cups for teat dipping instead of spraying, and practiced pre-and postdipping had significantly lower bulk tank somatic cell and/or bacterial counts. In conclusion, categorized bulk tank somatic cell and bacterial counts could serve as indicators and facilitate monitoring of herd udder health and milk

  19. Finite Element Analysis of Reinforced Concrete Plate Impacted by Block

    Institute of Scientific and Technical Information of China (English)

    LUO Xiaoyang; Pascal PERROTIN; YAN Quansheng

    2006-01-01

    A new concept of structurally dissipating rock-shed (SDR) was developed by the lab of Tonello IC and LOCIE-ESIGEC (France).To decide the dimension of the plate used in SDR,an ANSYS model which could simulate the impact of rock in the centre of the plate was established by Fabien Delhomme.By using this model,some finite element analyses are carried out in the present paper Firstly,a plate impacted by a block is numerically simulated,the numerical results obtained from different mesh sizes are compared and the accuracy of the finite element model is verified.Then,the dynamic response of the plate impacted at the boundary and in the medium part is computed.By analyzing the stress in rebar,the most dangerous region of impact of plate was found.For a rectangular plate,the most dangerous region is at the corner of the plate when a block drops in.Finally,the whole deformation process of the plate under dropping block was simulated and a simplified definition (effect zone) to describe the deformation process in different positions of plate was given.From this study,it is found that the impact only affects heavily within the effect zone.

  20. Longitudinal Analysis of Somatic Cell Count for Joint Genetic Evaluation of Mastitis and Recovery Liability

    DEFF Research Database (Denmark)

    Welderufael, Berihu Gebremedhin; de Koning, D J; Janss, Luc;

    Abstract Text: Better models of genetic evaluation for mastitis can be developed through longitudinal analysis of somatic cell count (SCC) which usually is used as a proxy for mastitis. Mastitis and recovery data with weekly observations of SCC were simulated for daughter groups of 60 and 240 per...

  1. BMI in relation to sperm count : an updated systematic review and collaborative meta-analysis

    NARCIS (Netherlands)

    Sermondade, N.; Faure, C.; Fezeu, L.; Shayeb, A. G.; Bonde, J. P.; Jensen, T. K.; Van Wely, M.; Cao, J.; Martini, A. C.; Eskandar, M.; Chavarro, J. E.; Koloszar, S.; Twigt, J. M.; Ramlau-Hansen, C. H.; Borges, E.; Lotti, F.; Steegers-Theunissen, R. P. M.; Zorn, B.; Polotsky, A. J.; La Vignera, S.; Eskenazi, B.; Tremellen, K.; Magnusdottir, E. V.; Fejes, I.; Hercberg, S.; Levy, R.; Czernichow, S.

    2013-01-01

    The global obesity epidemic has paralleled a decrease in semen quality. Yet, the association between obesity and sperm parameters remains controversial. The purpose of this report was to update the evidence on the association between BMI and sperm count through a systematic review with meta-analysis

  2. Everybody Counts, but Usually Just to 10! A Systematic Analysis of Number Representations in Children's Books

    Science.gov (United States)

    Powell, Sarah R.; Nurnberger-Haag, Julie

    2015-01-01

    Research Findings: Teachers and parents often use trade books to introduce or reinforce mathematics concepts. To date, an analysis of the early numeracy content of trade books has not been conducted. Consequently, this study evaluated the properties of numbers and counting within trade books. We coded 160 trade books targeted at establishing early…

  3. Everybody Counts, but Usually Just to 10! A Systematic Analysis of Number Representations in Children's Books

    Science.gov (United States)

    Powell, Sarah R.; Nurnberger-Haag, Julie

    2015-01-01

    Research Findings: Teachers and parents often use trade books to introduce or reinforce mathematics concepts. To date, an analysis of the early numeracy content of trade books has not been conducted. Consequently, this study evaluated the properties of numbers and counting within trade books. We coded 160 trade books targeted at establishing early…

  4. Latent segmentation based count models: Analysis of bicycle safety in Montreal and Toronto.

    Science.gov (United States)

    Yasmin, Shamsunnahar; Eluru, Naveen

    2016-10-01

    The study contributes to literature on bicycle safety by building on the traditional count regression models to investigate factors affecting bicycle crashes at the Traffic Analysis Zone (TAZ) level. TAZ is a traffic related geographic entity which is most frequently used as spatial unit for macroscopic crash risk analysis. In conventional count models, the impact of exogenous factors is restricted to be the same across the entire region. However, it is possible that the influence of exogenous factors might vary across different TAZs. To accommodate for the potential variation in the impact of exogenous factors we formulate latent segmentation based count models. Specifically, we formulate and estimate latent segmentation based Poisson (LP) and latent segmentation based Negative Binomial (LNB) models to study bicycle crash counts. In our latent segmentation approach, we allow for more than two segments and also consider a large set of variables in segmentation and segment specific models. The formulated models are estimated using bicycle-motor vehicle crash data from the Island of Montreal and City of Toronto for the years 2006 through 2010. The TAZ level variables considered in our analysis include accessibility measures, exposure measures, sociodemographic characteristics, socioeconomic characteristics, road network characteristics and built environment. A policy analysis is also conducted to illustrate the applicability of the proposed model for planning purposes. This macro-level research would assist decision makers, transportation officials and community planners to make informed decisions to proactively improve bicycle safety - a prerequisite to promoting a culture of active transportation.

  5. Detecting overdispersion in count data: A zero-inflated Poisson regression analysis

    Science.gov (United States)

    Afiqah Muhamad Jamil, Siti; Asrul Affendi Abdullah, M.; Kek, Sie Long; Nor, Maria Elena; Mohamed, Maryati; Ismail, Norradihah

    2017-09-01

    This study focusing on analysing count data of butterflies communities in Jasin, Melaka. In analysing count dependent variable, the Poisson regression model has been known as a benchmark model for regression analysis. Continuing from the previous literature that used Poisson regression analysis, this study comprising the used of zero-inflated Poisson (ZIP) regression analysis to gain acute precision on analysing the count data of butterfly communities in Jasin, Melaka. On the other hands, Poisson regression should be abandoned in the favour of count data models, which are capable of taking into account the extra zeros explicitly. By far, one of the most popular models include ZIP regression model. The data of butterfly communities which had been called as the number of subjects in this study had been taken in Jasin, Melaka and consisted of 131 number of subjects visits Jasin, Melaka. Since the researchers are considering the number of subjects, this data set consists of five families of butterfly and represent the five variables involve in the analysis which are the types of subjects. Besides, the analysis of ZIP used the SAS procedure of overdispersion in analysing zeros value and the main purpose of continuing the previous study is to compare which models would be better than when exists zero values for the observation of the count data. The analysis used AIC, BIC and Voung test of 5% level significance in order to achieve the objectives. The finding indicates that there is a presence of over-dispersion in analysing zero value. The ZIP regression model is better than Poisson regression model when zero values exist.

  6. Create Your Plate

    Medline Plus

    Full Text Available ... Carbohydrates Carbohydrate Counting Make Your Carbs Count Glycemic ... to manage portion control wherever you are. Now, our best-selling, sectioned to-go plate with easy-sealing ...

  7. A rapid method for counting nucleated erythrocytes on stained blood smears by digital image analysis

    Science.gov (United States)

    Gering, E.; Atkinson, C.T.

    2004-01-01

    Measures of parasitemia by intraerythrocytic hematozoan parasites are normally expressed as the number of infected erythrocytes per n erythrocytes and are notoriously tedious and time consuming to measure. We describe a protocol for generating rapid counts of nucleated erythrocytes from digital micrographs of thin blood smears that can be used to estimate intensity of hematozoan infections in nonmammalian vertebrate hosts. This method takes advantage of the bold contrast and relatively uniform size and morphology of erythrocyte nuclei on Giemsa-stained blood smears and uses ImageJ, a java-based image analysis program developed at the U.S. National Institutes of Health and available on the internet, to recognize and count these nuclei. This technique makes feasible rapid and accurate counts of total erythrocytes in large numbers of microscope fields, which can be used in the calculation of peripheral parasitemias in low-intensity infections.

  8. Biomechanical analysis of acromioclavicular joint dislocation treated with clavicle hook plates in different lengths.

    Science.gov (United States)

    Shih, Cheng-Min; Huang, Kui-Chou; Pan, Chien-Chou; Lee, Cheng-Hung; Su, Kuo-Chih

    2015-11-01

    Clavicle hook plates are frequently used in clinical orthopaedics to treat acromioclavicular joint dislocation. However, patients often exhibit acromion osteolysis and per-implant fracture after undergoing hook plate fixation. With the intent of avoiding future complications or fixation failure after clavicle hook plate fixation, we used finite element analysis (FEA) to investigate the biomechanics of clavicle hook plates of different materials and sizes when used in treating acromioclavicular joint dislocation. Using finite element analysis, this study constructed a model comprising four parts: clavicle, acromion, clavicle hook plate and screws, and used the model to simulate implanting different types of clavicle hook plates in patients with acromioclavicular joint dislocation. Then, the biomechanics of stainless steel and titanium alloy clavicle hook plates containing either six or eight screw holes were investigated. The results indicated that using a longer clavicle hook plate decreased the stress value in the clavicle, and mitigated the force that clavicle hook plates exert on the acromion. Using a clavicle hook plate material characterized by a smaller Young's modulus caused a slight increase in the stress on the clavicle. However, the external force the material imposed on the acromion was less than the force exerted on the clavicle. The findings of this study can serve as a reference to help orthopaedic surgeons select clavicle hook plates.

  9. Deep hard X-ray source counts from a fluctuation analysis of ASCA SIS images

    CERN Document Server

    Gendreau, K C; Fabian, A C

    1997-01-01

    An analysis of the spatial fluctuations in 15 deep ASCA SIS0 images has been conducted in order to probe the 2-10 keV X-ray source counts down to a flux limit 2E-14 erg/cm2/s. Special care has been taken in modelling the fluctuations in terms of the sensitivity maps of every one of the 16 regions (5.6 x 5.6 arcmin2 each) in which the SIS0 has been divided, by means of raytracing simulations with improved optical constants in the X-ray telescope. The very extended `side lobes' (extending up to a couple of degrees) exhibited by these sensitivity maps make our analysis sensitive to both faint on-axis sources and brighter off-axis ones, the former being dominant. The source counts in the range (2-12)E-14 erg/cm2/s are found to be close to a euclidean form which extrapolates well to previous results from higher fluxes and in reasonable agreement with some recent ASCA surveys. However, our results disagree with the deep survey counts by Georgantopoulos et al. (1997). The possibility that the source counts flatten t...

  10. The postbuckling analysis of laminated circular plate with elliptic delamination

    Science.gov (United States)

    Chen, Deliang; Chen, Changping; Fu, Yiming

    2011-01-01

    Based on the Von Karman plate theory, considering the effect of transverse shear deformation, and using the method of the dissociated three regions, the postbuckling governing equations for the axisymmetric laminated circular plates with elliptical delamination are derived. By using the orthogonal point collocation method, the governing equations, boundary conditions and continuity conditions are transformed into a group of nonlinear algebraically equation and the equations are solved with the alternative method. In the numerical examples, the effects of various elliptical in shape, delamination depth and different material properties on buckling and postbuckling of the laminated circular plates are discussed and the numerical results are compared with available data.

  11. Free vibration analysis of Mindlin plates with linearly varying thickness

    Science.gov (United States)

    Aksu, G.; Al-Kaabi, S. A.

    1987-12-01

    A method based on the variational principles in conjunction with the finite difference technique is applied to examine the free vibration characteristics of isotropic rectangular plates of linearly varying thickness by including the effects of transverse shear deformation and rotary inertia. The validity of the present approach is demonstrated by comparing the results with other solutions proposed for plates with uniform and linearly varying thickness. Natural frequencies and mode shapes of Mindlin plates with simply supported and clamped edges are determined for various values of relative thickness ratio and the taper thickness constant.

  12. Overstating the evidence – double counting in meta-analysis and related problems

    Directory of Open Access Journals (Sweden)

    Senn Stephen J

    2009-02-01

    Full Text Available Abstract Background The problem of missing studies in meta-analysis has received much attention. Less attention has been paid to the more serious problem of double counting of evidence. Methods Various problems in overstating the precision of results from meta-analyses are described and illustrated with examples, including papers from leading medical journals. These problems include, but are not limited to, simple double counting of the same studies, double counting of some aspects of the studies, inappropriate imputation of results, and assigning spurious precision to individual studies. Results Some suggestions are made as to how the quality and reliability of meta-analysis can be improved. It is proposed that the key to quality in meta-analysis lies in the results being transparent and checkable. Conclusion Existing quality check lists for meta-analysis do little to encourage an appropriate attitude to combining evidence and to statistical analysis. Journals and other relevant organisations should encourage authors to make data available and make methods explicit. They should also act promptly to withdraw meta-analyses when mistakes are found.

  13. Vibration analysis of orthotropic circular and elliptical nano-plates embedded in elastic medium based on nonlocal Mindlin plate theory and using Galerkin method

    Energy Technology Data Exchange (ETDEWEB)

    Anjomshoa, Amin; Tahani, Masoud [Ferdowsi University, Mashhad (Iran, Islamic Republic of)

    2016-06-15

    In the present study a continuum model based on the nonlocal elasticity theory is developed for free vibration analysis of embedded ortho tropic thick circular and elliptical nano-plates rested on an elastic foundation. The elastic foundation is considered to behave like a Pasternak type of foundations. Governing equations for vibrating nano-plate are derived according to the Mindlin plate theory in which the effects of shear deformations of nano-plate are also included. The Galerkin method is then employed to obtain the size dependent natural frequencies of nano-plate. The solution procedure considers the entire nano-plate as a single super-continuum element. Effect of nonlocal parameter, lengths of nano-plate, aspect ratio, mode number, material properties, thickness and foundation on circular frequencies are investigated. It is seen that the nonlocal frequencies of the nano-plate are smaller in comparison to those from the classical theory and this is more pronounced for small lengths and higher vibration modes. It is also found that as the aspect ratio increases or the nanoplate becomes more elliptical, the small scale effect on natural frequencies increases. Further, it is observed that the elastic foundation decreases the influence of nonlocal parameter on the results. Since the effect of shear deformations plays an important role in vibration analysis and design of nano-plates, by predicting smaller values for fundamental frequencies, the study of these nano-structures using thick plate theories such as Mindlin plate theory is essential.

  14. Electrostatic and Small-Signal Analysis of CMUTs With Circular and Square Anisotropic Plates.

    Science.gov (United States)

    Funding la Cour, Mette; Christiansen, Thomas Lehrmann; Jensen, Jørgen Arendt; Thomsen, Erik Vilain

    2015-08-01

    Traditionally, capacitive micromachined ultrasonic transducers (CMUTs) are modeled using the isotropic plate equation, and this leads to deviations between analytical calculations and finite element modeling (FEM). In this paper, the deflection is calculated for both circular and square plates using the full anisotropic plate equation. It is shown that the anisotropic calculations match excellently with FEM, whereas an isotropic approach causes up to 10% deviations in deflection. For circular plates, an exact solution can be found. For square plates using the Galerkin method, and utilizing the symmetry of the silicon crystal, a compact and accurate expression for the deflection can be obtained. The deviation from FEM in center deflection is white light interferometer. Fitting the plate parameter for the anisotropic calculated deflection to the measurement, a deviation of 0.07% is seen. Electrostatic and small-signal dynamic analysis are performed using energy considerations including anisotropy. The stable position, effective spring constant, pullin distance, and pull-in voltage are found for both circular and square anisotropic plates, and the pressure dependence is included by comparison with the corresponding analysis for a parallel plate. Measurements on fabricated devices with both circular and square plates subjected to increasing bias voltage are performed, and it is observed that the models including anisotropic effects are within the uncertainty interval of the measurements. Finally, a lumped element small-signal model for both circular and square anisotropic plates is derived to describe the dynamics of the CMUT.

  15. Semi-Analytical Finite Strip Transfer Matrix Method for Buckling Analysis of Rectangular Thin Plates

    Directory of Open Access Journals (Sweden)

    Li-Ke Yao

    2015-01-01

    Full Text Available Plates and shells are main components of modern engineering structures, whose buckling analysis has been focused by researchers. In this investigation, rectangular thin plates with loaded edges simply supported can be discretized by semi-analytical finite strip technology. Then the control equations of the strip elements of the buckling plate will be rewritten as the transfer equations by transfer matrix method. A new approach, namely semi-analytical Finite Strip Transfer Matrix Method, is developed for the buckling analysis of plates. This method requires no global stiffness matrix of the system, reduces the system matrix order, and improves the computational efficiency. Comparing with some theoretical results and FEM’s results of two illustrations (the plates and the ribbed plates under six boundary conditions, the method is proved to be reliable and effective.

  16. Experimental study and analysis of a novel multi-media plate heat exchanger

    Institute of Scientific and Technical Information of China (English)

    SONG JiWei; WANG Fei; CHENG Lin

    2012-01-01

    The experimental study and analysis of a novel multi-media plate heat exchanger were performed in this paper.This novel multi-media plate heat exchanger was self-developed during the process of the investigation and design of the alpha magnetic spectrometer (AMS) thermal system.The plate of this kind of novel plate heat exchanger is formed by discontinuous structure wave consisting of convex sphere and concave sphere,its heat transfer performance is better than that of the BRI chevron plate heat exchanger,and its resistance characteristics are superior to those of the nornally used 60-degree plate heat exchanger.Furthermore,the mechanism analysis of heat transfer enhancement shows that the spherical wave structure can reduce the local field synergy angle,so as to improve the field synergy degree of velocity vector and temperature gradient vector.

  17. Spectroscopic and ultrastructural analysis of fluorhydroxyapatite-coated titanium plates

    Energy Technology Data Exchange (ETDEWEB)

    Martini, D.; Ottani, V.; Ruggeri, A. [Bologna Univ. (Italy). Dipt. di Scienze Anatomiche e Fisiopatologia dell' Apparato Locomotore; Raspanti, M. [Insubria Univ. (Italy). Dipt. di Scienze Cliniche e Biologiche; Monti, P.; Taddei, P.; Tinti, A.; Fragnano, C. [Bologna Univ. (Italy). Dipt. di Biochimica ' G. Moruzzi'

    2001-07-01

    This study aimed to describe the chemical, physical and morphological features of the surface of titanium plates coated with porous titanium and fluorhydroxyapatite for surgical implantation. Although the plasma flame spray coating process partially altered the original chemical and physical characteristics of the coatings, the surface morphology of the coated plates seems very favourable for bone integration of the implant. Immersion in simulated body fluid for ten weeks failed to disclose signs of implant dissolution or impaired surface composition. (orig.)

  18. Counting Penguins.

    Science.gov (United States)

    Perry, Mike; Kader, Gary

    1998-01-01

    Presents an activity on the simplification of penguin counting by employing the basic ideas and principles of sampling to teach students to understand and recognize its role in statistical claims. Emphasizes estimation, data analysis and interpretation, and central limit theorem. Includes a list of items for classroom discussion. (ASK)

  19. Thermal buckling analysis of truss-core sandwich plates

    Institute of Scientific and Technical Information of China (English)

    陈继伟; 刘咏泉; 刘伟; 苏先樾

    2013-01-01

    Truss-core sandwich plates have received much attention in virtue of the high values of strength-to-weight and stiffness-to-weight as well as the great ability of impulse-resistance recently. It is necessary to study the stability of sandwich panels under the influence of the thermal load. However, the sandwich plates are such complex three-dimensional (3D) systems that direct analytical solutions do not exist, and the finite element method (FEM) cannot represent the relationship between structural parameters and mechanical properties well. In this paper, an equivalent homogeneous continuous plate is idealized by obtaining the effective bending and transverse shear stiffness based on the characteristics of periodically distributed unit cells. The first order shear deformation theory for plates is used to derive the stability equation. The buckling temperature of a simply supported sandwich plate is given and verified by the FEM. The effect of related parameters on mechanical properties is investigated. The geometric parameters of the unit cell are optimized to attain the maximum buckling temperature. It is shown that the optimized sandwich plate can improve the resistance to thermal buckling significantly.

  20. Statistical analysis of data from dilution assays with censored correlated counts.

    Science.gov (United States)

    Quiroz, Jorge; Wilson, Jeffrey R; Roychoudhury, Satrajit

    2012-01-01

    Frequently, count data obtained from dilution assays are subject to an upper detection limit, and as such, data obtained from these assays are usually censored. Also, counts from the same subject at different dilution levels are correlated. Ignoring the censoring and the correlation may provide unreliable and misleading results. Therefore, any meaningful data modeling requires that the censoring and the correlation be simultaneously addressed. Such comprehensive approaches of modeling censoring and correlation are not widely used in the analysis of dilution assays data. Traditionally, these data are analyzed using a general linear model on a logarithmic-transformed average count per subject. However, this traditional approach ignores the between-subject variability and risks, providing inconsistent results and unreliable conclusions. In this paper, we propose the use of a censored negative binomial model with normal random effects to analyze such data. This model addresses, in addition to the censoring and the correlation, any overdispersion that may be present in count data. The model is shown to be widely accessible through the use of several modern statistical software. Copyright © 2012 John Wiley & Sons, Ltd.

  1. Theoretical analysis based on fundamental functions of thin plate and experimental measurement for vibration characteristics of a plate coupled with liquid

    Science.gov (United States)

    Liao, Chan-Yi; Wu, Yi-Chuang; Chang, Ching-Yuan; Ma, Chien-Ching

    2017-04-01

    This study combined theoretical, experimental, and numerical analysis to investigate the vibration characteristics of a thin rectangular plate positioned horizontally at the bottom of a rectangular container filled with liquid. Flow field pressure was derived using an equation governing the behavior of incompressible fluids. Analytic solutions to vibrations in a thin plate in air served as the fundamental function of the thin plate coupled with liquid. We then used liquid pressure, and the out-of-plane deflection of the thin plate for the construction of frequency response functions for the analysis of vibration characteristics in the liquid-plate coupling system. Two experimental methods were employed to measure the vibration characteristics of the thin plate immersed in water. The first involved using sensors of polyvinylidene difluoride (PVDF) to measure transient signals of fluid-plate system subjected an impact at the thin plate. These were then converted to the frequency domain in order to obtain the resonant frequencies of the fluid-plate coupling system. The second method was amplitude-fluctuation electronic speckle pattern interferometry (AF-ESPI), which was used to measure the dynamic characteristics of the thin plate in the flow field. This method was paired with the image processing techniques, temporal speckle pattern interferometry (TSPI) and temporal standard deviation (TSTD), to obtain clear mode shapes of the thin plate and resonant frequencies. Comparison of the results from theoretical analysis, finite element method, and experimental measurements confirmed the accuracy of our theoretical analysis, which was superior to the conventional approach based on beam mode shape functions. The experimental methods proposed in this study can be used to measure the resonant frequencies of underwater thin plates, and clear mode shapes can be obtained using AF-ESPI. Our results indicate that the resonant frequencies of thin plates underwater are lower than

  2. Quantitative analysis of changes in actin microfilament contribution to cell plate development in plant cytokinesis

    Directory of Open Access Journals (Sweden)

    Sano Toshio

    2008-07-01

    Full Text Available Abstract Background Plant cells divide by the formation of new cross walls, known as cell plates, from the center to periphery of each dividing cell. Formation of the cell plate occurs in the phragmoplast, a complex structure composed of membranes, microtubules (MTs and actin microfilaments (MFs. Disruption of phragmoplast MTs was previously found to completely inhibit cell plate formation and expansion, indicative of their crucial role in the transport of cell plate membranes and materials. In contrast, disruption of MFs only delays cell plate expansion but does not completely inhibit cell plate formation. Despite such findings, the significance and molecular mechanisms of MTs and MFs remain largely unknown. Results Time-sequential changes in MF-distribution were monitored by live imaging of tobacco BY-2 cells stably expressing the GFP-actin binding domain 2 (GFP-ABD2 fusion protein, which vitally co-stained with the endocytic tracer, FM4-64, that labels the cell plate. During cytokinesis, MFs accumulated near the newly-separated daughter nuclei towards the emerging cell plate, and subsequently approached the expanding cell plate edges. Treatment with an actin polymerization inhibitor caused a decrease in the cell plate expansion rate, which was quantified using time-lapse imaging and regression analysis. Our results demonstrated time-sequential changes in the contribution of MFs to cell plate expansion; MF-disruption caused about a 10% decrease in the cell plate expansion rate at the early phase of cytokinesis, but about 25% at the late phase. MF-disruption also caused malformation of the emerging cell plate at the early phase, indicative of MF involvement in early cell plate formation and expansion. The dynamic movement of endosomes around the cell plate was also inhibited by treatment with an actin polymerization inhibitor and a myosin ATPase inhibitor, respectively. Furthermore, time-lapse imaging of the endoplasmic reticulum (ER revealed

  3. Using Count Data and Ordered Models in National Forest Recreation Demand Analysis

    Science.gov (United States)

    Simões, Paula; Barata, Eduardo; Cruz, Luis

    2013-11-01

    This research addresses the need to improve our knowledge on the demand for national forests for recreation and offers an in-depth data analysis supported by the complementary use of count data and ordered models. From a policy-making perspective, while count data models enable the estimation of monetary welfare measures, ordered models allow for the wider use of the database and provide a more flexible analysis of data. The main purpose of this article is to analyse the individual forest recreation demand and to derive a measure of its current use value. To allow a more complete analysis of the forest recreation demand structure the econometric approach supplements the use of count data models with ordered category models using data obtained by means of an on-site survey in the Bussaco National Forest (Portugal). Overall, both models reveal that travel cost and substitute prices are important explanatory variables, visits are a normal good and demographic variables seem to have no influence on demand. In particular, estimated price and income elasticities of demand are quite low. Accordingly, it is possible to argue that travel cost (price) in isolation may be expected to have a low impact on visitation levels.

  4. Analysis of interlaminar stress and nonlinear dynamic response for composite laminated plates with interfacial damage

    Science.gov (United States)

    Zhu, F. H.; Fu, Y. M.

    2008-12-01

    By considering the effect of interfacial damage and using the variation principle, three-dimensional nonlinear dynamic governing equations of the laminated plates with interfacial damage are derived based on the general six-degrees-of-freedom plate theory towards the accurate stress analysis. The solutions of interlaminar stress and nonlinear dynamic response for a simply supported laminated plate with interfacial damage are obtained by using the finite difference method, and the results are validated by comparison with the solution of nonlinear finite element method. In numerical calculations, the effects of interfacial damage on the stress in the interface and the nonlinear dynamic response of laminated plates are discussed.

  5. EXACT SOLUTION FOR TEMPERATURE-DEPENDENT BUCKLING ANALYSIS OF FG-CNT-REINFORCED MINDLIN PLATES

    Directory of Open Access Journals (Sweden)

    Seyed Mohammad Mousavi

    2016-03-01

    Full Text Available This research deals with the buckling analysis of nanocomposite polymeric temperature-dependent plates reinforced by single-walled carbon nanotubes (SWCNTs. For the carbon-nanotube reinforced composite (CNTRC plate, uniform distribution (UD and three types of functionally graded (FG distribution patterns of SWCNT reinforcements are assumed. The material properties of FG-CNTRC plate are graded in the thickness direction and estimated based on the rule of mixture. The CNTRC is located in a elastic medium which is simulated with temperature-dependent Pasternak medium. Based on orthotropic Mindlin plate theory, the governing equations are derived using Hamilton’s principle and solved by Navier method. The influences of the volume fractions of carbon nanotubes, elastic medium, temperature and distribution type of CNTs are considered on the buckling of the plate. Results indicate that CNT distribution close to top and bottom are more efficient than those distributed nearby the mid-plane for increasing the stiffness of plates.

  6. Energy flow analysis of out-of-plane vibration in coplanar coupled finite Mindlin plates

    Directory of Open Access Journals (Sweden)

    Park Young-Ho

    2015-01-01

    Full Text Available In this paper, an Energy Flow Analysis (EFA for coplanar coupled Mindlin plates was performed to estimate their dynamic responses at high frequencies. Mindlin plate theory can consider the effects of shear distortion and rotatory inertia, which are very important at high frequencies. For EFA for coplanar coupled Mindlin plates, the wave transmission and reflection relationship for progressing out-of-plane waves (out-of-plane shear wave, bending dominant flexural wave, and shear dominant flexural wave in coplanar coupled Mindlin plates was newly derived. To verify the validity of the EFA results, numerical analyses were performed for various cases where coplanar coupled Mindlin plates are excited by a harmonic point force, and the energy flow solutions for coplanar coupled Mindlin plates were compared with the classical solutions in the various conditions.

  7. Analysis of thin plates by the weak form quadrature element method

    Institute of Scientific and Technical Information of China (English)

    ZHONG HongZhi; YUE ZhiGuang

    2012-01-01

    The recently proposed weak form quadrature element method (QEM) is applied to flexural and vibrational analysis of thin plates.The integrals involved in the variational description of a thin plate are evaluated by an efficient numerical scheme and the partial derivatives at the integration sampling points are then approximated using differential quadrature analogs.Neither the grid pattern nor the number of nodes is fixed,being adjustable according to convergence need.The C1 continuity conditions characterizing the thin plate theory are discussed and the robustness of the weak form quadrature element for thin plates against shape distortion is examined.Examples are presented and comparisons with analytical solutions and the results of the finite element method are made to demonstrate the convergence and computational efficiency of the weak form quadrature element method.It is shown that the present formulation is applicable to thin plates with varying thickness as well as uniform plates.

  8. Elastic buckling analysis of corroded stiffened plates with irregular surfaces

    Indian Academy of Sciences (India)

    Ahmad Rahbar-Ranji

    2015-02-01

    Numerical simulation is used to study the influence of corrosion damage in stiffened plates focusing on elastic buckling strength. Three-dimensional specta are used to simulate geometries of corroded surfaces and finite element method is employed for computing Euler stress of stiffened plates. The influence of corrosion patterns, amount of corrosion loss and roughness of surface are investigated. Ratio of Euler stress of corroded stiffened plate over Euler stress of un-corroded stiffened plate is used to characterize the effects of corrosion on reduction of buckling strength. Results show that reduction of buckling strength is very sensitive to the amount of corrosion loss and roughness of surface, but less sensitive to the location of corroded region. The potential for decrease in buckling strength as a consequence of corrosion is found to depend on the dominant buckling mode. Residual buckling strength is reduced by as much as 12% for the interaction of plate-web-torsional buckling mode, and by 2% for column buckling.

  9. ELASTIC DYNAMIC ANALYSIS OF MODERATELY THICK PLATE USING MESHLESS LRPIM

    Institute of Scientific and Technical Information of China (English)

    Ping Xia; Shuyao Long; Hongxue Cui

    2009-01-01

    A meshless local radial point interpolation method (LRPIM) for solving elastic dy-namic problems of moderately thick plates is presented in this paper. The discretized system equation of the plate is obtained using a locally weighted residual method. It uses a radial basis function (RBF) coupled with a polynomial basis function as a trial function, and uses the quartic spline function as a test function of the weighted residual method. The shape function has the properties of the Kronecker delta function, and no additional treatment is done to impose essen-tial boundary conditions. The Newmark method for solving the dynamic problem is adopted in computation. Effects of sizes of the quadrature sub-domain and influence domain on the dynamic properties are investigated. The numerical results show that the presented method can give quite accurate results for the elastic dynamic problem of the moderately thick plate.

  10. Outcome Analysis of Locking Plate Fixation in Proximal Humerus Fracture

    Science.gov (United States)

    Pathak, Abhishek; Gaur, Sanjiv

    2016-01-01

    Introduction Proximal humerus fractures account for approximately 5% of all fractures. Stable minimally displaced fractures can be treated nonoperatively but the management of displaced fractures remain controversial with various modalities of treatment available. Locking plates provide stable fixation and enable early postoperative mobilization specially in osteoporotic proximal humerus fracture. Aim To evaluate the functional outcome of locking plate fixation and to compare the results of two approaches used for fixation. Materials and Methods This prospective study was conducted at a tertiary level hospital between September 2011 to December 2013. PHILOS plates were used for internal fixation of displaced proximal humerus fractures Neer’s type 2 part, 3 part and 4 part fractures on 26 patients (M/F ratio 1.36:1; mean age 46 years). According to Neer classification, 5,12 and 9 patients had displaced 2, 3 and 4 part fractures respectively. Deltopectoral and deltoid splitting approaches were used for fixation on 13 patients each. Functional outcome was assessed using Constant-Murley shoulder score. Graphpad software version 6.0 was used with Chi-square test and Fisher-exact test are used to compare data. The p-value .05) and all fractures were united. Conclusion Our study demonstrates that locking plate fixation gives good functional outcomes in treatment of proximal humerus fractures. There was no significant difference in the two approaches used for exposure. Our results are comparable to various studies conducted by other authors which states that locking plates provide better functional and radiological outcomes as compared to other fixation methods like Tension band wiring, percutaneous K-wire fixation, non-locking plates, intramedullary nails. PMID:27656515

  11. Analysis and Design of Circular Plate MR Fluids Brake

    Institute of Scientific and Technical Information of China (English)

    Yang Yan; Lin Chang-Hua; Li Hui; Zhou Jing

    2004-01-01

    A magnetorheological (MR) fluids brake is a device to achieve brake by shear force of MR fluids. A MR rotary brake has the property that its braking torque changes quickly in response to an external magnetic field. In this study, the design method of the circular plate MR fluids brake is investigated theoretically. The equation of the torque transmitted by the MR fluids in the brake is derived to provide the theoretical foundation in the design of the brake. Based on this equation, after mathematically manipulated, the calculations of the volume, thickness and width of the MR fluids within the circular plate MR fluids brake are yield.

  12. RCS Analysis of Plate Geometries, parts 1 and 2

    Science.gov (United States)

    Balanis, Constantine A.; Polka, Lesley A.; Polycarpou, Anastasis C.

    1993-01-01

    High-frequency techniques for Radar Cross Section (RCS) prediction of plate geometries and a physical optics/equivalent currents model for the RCS of trihedral corner reflectors are addressed. In part 1, a Uniform Theory of Diffraction (UTD) model for the principal-plane radar cross section (RCS) of a perfectly conducting, rectangular plate coated on one side with an electrically thin, lossy dielectric is presented. In part 2, the scattering in the interior regions of both square and triangular trihedral corner reflectors are examined.

  13. ACCURACY ANALYSIS OF 12-PARAMETERRECTANGULAR PLATE ELEMENTS WITH GEOMETRIC SYMMETRY

    Institute of Scientific and Technical Information of China (English)

    SHI Dongyang; CHEN Shaochun

    2000-01-01

    In this paper, it is proved that the double set parameter rectangular plate elements with geometric symmetry possess a very special convergence property, i.e., the consistency error due to nonconformity is of order O(h2) which is one order higher than that of ACM element and rectangular generalized conforming element proposed by Y. Q.Long, although all these elements have the same asympotical rate of convergence O(h)in the energy norm. This particular property seems to be never seen before for other nonconforming rectangular plate elements.

  14. A mixed finite element for the analysis of laminated plates

    Science.gov (United States)

    Putcha, N. S.; Reddy, J. N.

    1983-01-01

    A new mixed shear-flexible finite element based on the Hellinger-Reissner's variational principle is developed. The element is constructed using a mixed formulation of the shear deformation theory of laminated composite plates, and consists of three displacements, two shear rotations, and three moments as the independent degrees of freedom. The numerical convergence and accuracy characteristics of the element are investigated for bending of laminated anisotropic composite plates. The element is relatively simple to construct and has better accuracy and convergence features when compared to other conventional finite elements.

  15. Sensitivity analysis of the GNSS derived Victoria plate motion

    Science.gov (United States)

    Apolinário, João; Fernandes, Rui; Bos, Machiel

    2014-05-01

    estimated trend (Williams 2003, Langbein 2012). Finally, our preferable angular velocity estimation is used to evaluate the consequences on the kinematics of the Victoria block, namely the magnitude and azimuth of the relative motions with respect to the Nubia and Somalia plates and their tectonic implications. References Agnew, D. C. (2013). Realistic simulations of geodetic network data: The Fakenet package, Seismol. Res. Lett., 84 , 426-432, doi:10.1785/0220120185. Blewitt, G. & Lavallee, D., (2002). Effect of annual signals on geodetic velocity, J. geophys. Res., 107(B7), doi:10.1029/2001JB000570. Bos, M.S., R.M.S. Fernandes, S. Williams, L. Bastos (2012) Fast Error Analysis of Continuous GNSS Observations with Missing Data, Journal of Geodesy, doi: 10.1007/s00190-012-0605-0. Bos, M.S., L. Bastos, R.M.S. Fernandes, (2009). The influence of seasonal signals on the estimation of the tectonic motion in short continuous GPS time-series, J. of Geodynamics, j.jog.2009.10.005. Fernandes, R.M.S., J. M. Miranda, D. Delvaux, D. S. Stamps and E. Saria (2013). Re-evaluation of the kinematics of Victoria Block using continuous GNSS data, Geophysical Journal International, doi:10.1093/gji/ggs071. Langbein, J. (2012). Estimating rate uncertainty with maximum likelihood: differences between power-law and flicker-random-walk models, Journal of Geodesy, Volume 86, Issue 9, pp 775-783, Williams, S. D. P. (2003). Offsets in Global Positioning System time series, J. Geophys. Res., 108, 2310, doi:10.1029/2002JB002156, B6.

  16. Surgical swab counting: a qualitative analysis from the perspective of the scrub nurse.

    Science.gov (United States)

    D'Lima, D; Sacks, M; Blackman, W; Benn, J

    2014-05-01

    The aim of the study was to conduct a qualitative exploration of the sociotechnical processes underlying retained surgical swabs, and to explore the fundamental reasons why the swab count procedure and related protocols fail in practice. Data was collected through a set of 27 semistructured qualitative interviews with scrub nurses from a large, multi-site teaching hospital. Interview transcripts were analysed using established constant comparative methods, moving between inductive and deductive reasoning. Key findings were associated with interprofessional perspectives, team processes and climate and responsibility for the swab count. The analysis of risk factors revealed that perceived social and interprofessional issues played a significant role in the reliability of measures to prevent retained swabs. This work highlights the human, psychological and organisational factors that impact upon the reliability of the process and gives rise to recommendations to address contextual factors and improve perioperative practice and training.

  17. Analysis on approach of safeguards implementation at research reactor handling item count and bulk material

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyun Jo; Lee, Sung Ho; Lee, Byung Doo; Jung, Juang [KAERI, Daejeon (Korea, Republic of)

    2016-05-15

    KiJang research reactor (KJRR) will be constructed to produce the radioisotope such as Mo-99 etc., provide the neutron transmutation doping (NTD) service of silicon, and develop the core technologies of research reactor. In this paper, the features of the process and nuclear material flow are reviewed and the material balance area (MBA) and key measurement point (KMP) are established based on the nuclear material flow. Also, this paper reviews the approach on safeguards and nuclear material accountancy at the facility level for Safeguards-by-Design at research reactor handling item count and bulk material. In this paper, MBA and KMPs are established through the analysis on facility features and major process at KJRR handling item count and bulk material. Also, this paper reviews the IAEA safeguards implementation and nuclear material accountancy at KJRR. It is necessary to discuss the safeguards approach on the fresh FM target assemblies and remaining uranium in the intermediate level liquid wastes.

  18. Detection methods of aerobic plate count on food microbiological proficiency testing%食品微生物能力验证样品菌落总数检验方法

    Institute of Scientific and Technical Information of China (English)

    肖剑; 李慧琴; 陈楷; 梁美丹; 林秀敏

    2014-01-01

    目的:探讨菌落总数能力验证的检验方法及注意事项。方法采用PCA直接倾注法、增加覆盖一层琼脂培养基的方法、添加 TTC 方法、陶瓦盖代替平皿盖的方法同时进行试验,并采用提前观察、增加观察的频率和培养时间,加强计数准确性。结果在前期培养过程中加强观察能够有助于计数,而延迟培养时间则可避免迟缓生长的细菌漏检;采用含有0.5%TTC 培养基进行培养或陶瓦盖平板法能有效抑制蔓延菌生长,且不影响总体计数结果。结论采用多种检验方法有助于菌落总数能力验证试验结果的准确报告。%ObjectiveTodiscuss the methods and precaution of aerobic plate count proficiency testing. MethodsFour methods were tested including pouring plate count agar directly, covering a layer of agar me-dium, adding TTC and using terracotta cover instead of petri dish. Meanwhile, using the early observation, in-creasing the frequency of observation and the time of cultivation to strengthen count accuracy.Results The results showed that the accuracy of results during the pre-cultivation counting was improved, and the missing of slow growing bacteria extending the time of cultivation was also avoided. In addition, it was effective to inhibit the spread of bacteria by culturing with the medium containing 0.5%TTC or using terracotta cover, and these methods did not affect the overall count results.Conclusion It is conductive to get accurate results by variety of methods for aerobic plate count proficiency testing.

  19. Nonlocal Elasticity Theory for Transient Analysis of Higher-Order Shear Deformable Nanoscale Plates

    Directory of Open Access Journals (Sweden)

    Woo-Young Jung

    2014-01-01

    Full Text Available The small scale effect on the transient analysis of nanoscale plates is studied. The elastic theory of the nano-scale plate is reformulated using Eringen’s nonlocal differential constitutive relations and higher-order shear deformation theory (HSDT. The equations of motion of the nonlocal theories are derived for the nano-scale plates. The Eringen’s nonlocal elasticity of Eringen has ability to capture the small scale effects and the higher-order shear deformation theory has ability to capture the quadratic variation of shear strain and consequently shear stress through the plate thickness. The solutions of transient dynamic analysis of nano-scale plate are presented using these theories to illustrate the effect of nonlocal theory on dynamic response of the nano-scale plates. On the basis of those numerical results, the relations between nonlocal and local theory are investigated and discussed, as are the nonlocal parameter, aspect ratio, side-to-thickness ratio, nano-scale plate size, and time step effects on the dynamic response. In order to validate the present solutions, the reference solutions are employed and examined. The results of nano-scale plates using the nonlocal theory can be used as a benchmark test for the transient analysis.

  20. A novel titanium dioxide-polydimethylsiloxane plate for phosphopeptide enrichment and mass spectrometry analysis.

    Science.gov (United States)

    Chen, Chao-Jung; Lai, Chien-Chen; Tseng, Mei-Chun; Liu, Yu-Ching; Liu, Yu-Huei; Chiou, Liang-Wei; Tsai, Fuu-Jen

    2014-02-17

    The phosphorylation of proteins is a major post-translational modification that is required for the regulation of many cellular processes and activities. Mass spectrometry signals of low-abundance phosphorylated peptides are commonly suppressed by the presence of abundant non-phosphorylated peptides. Therefore, one of the major challenges in the detection of low-abundance phosphopeptides is their enrichment from complex peptide mixtures. Titanium dioxide (TiO2) has been proven to be a highly efficient approach for phosphopeptide enrichment and is widely applied. In this study, a novel TiO2 plate was developed by coating TiO2 particles onto polydimethylsiloxane (PDMS)-coated MALDI plates, glass, or plastic substrates. The TiO2-PDMS plate (TP plate) could be used for on-target MALDI-TOF analysis, or as a purification plate on which phosphopeptides were eluted out and subjected to MALDI-TOF or nanoLC-MS/MS analysis. The detection limit of the TP plate was ∼10-folds lower than that of a TiO2-packed tip approach. The capacity of the ∼2.5 mm diameter TiO2 spots was estimated to be ∼10 μg of β-casein. Following TiO2 plate enrichment of SCC4 cell lysate digests and nanoLC-MS/MS analysis, ∼82% of the detected proteins were phosphorylated, illustrating the sensitivity and effectiveness of the TP plate for phosphoproteomic study.

  1. Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque counting using imaging analysis

    Directory of Open Access Journals (Sweden)

    Quiroz Jorge

    2005-11-01

    Full Text Available Abstract Background Respiratory syncytial virus (RSV is the major cause of lower respiratory tract infection in infants and young children. Although several experimental RSV vaccines are under investigation, immuno therapy is the only treatment currently available. In assessing the immunogenicity of various vaccine formulations, a plaque reduction neutralization assay for the evaluation of RSV neutralizing antibody has been widely used. The method produces reliable results, but it is tedious and labor intensive as it relies on manual counting by laboratory personnel. To facilitate evaluation of phase II and phase III vaccine clinical trials, a more rapid, reliable and efficient neutralization assay is needed. Results An improved microneutralization assay for quantifying RSV neutralizing antibodies was developed using an ImmunoSpot® Series I Analyzer (Cellular Technology Ltd., Cleveland, OH for automated plaque counting. The method is an improvement of the established classical microneutralization assay in which immunostained plaques on transparent tissue culture plates are counted manually under a dissecting microscope. Image analyzer technology allows for fully automated counting of plaques distributed throughout an entire well. Adjustments, such as the use of opaque tissue culture plates and the TMB substrate, True Blue™ (KPL, Gaithersburg, MD, were required to adapt the assay for optimal detection of plaques by the image analyzer. The suitability and the accuracy of the method for counting RSV plaques were determined by comparative testing of a reference serum and two control sera by manual and automated counting methods. The results showed that the two methods were highly correlated (R = 0.9580 and the titers generated by them were within two-fold. Conclusion Our results demonstrate that the semi-automated assay is rapid and reliable. It provides results within two fold to the classical plaque microneutralization assay and is readily

  2. Large deflection analysis of skew plates under uniformly distributed ...

    African Journals Online (AJOL)

    user

    load-deflection plane to show the effect of skew angle, aspect ratio and boundary condition. ... by edge 1, 2, 3 and 4 on the plate boundaries in Figures 1(a)-1(d). ... one-dimensional coordinate functions along normalized coordinate are shown ...

  3. Rigid-Plastic Post-Buckling Analysis of Columns and Quadratic Plates

    DEFF Research Database (Denmark)

    Jönsson, Jeppe

    2008-01-01

    The objective of this paper is to show the application of a novel approach to the rigid plastic hinge and yield line theory in post-buckling analysis of slender plates and columns. The upper bound theorem of plasticity theory and the associated flow law of plasticity are used to find...... of the post-buckling behaviour. The rigid plastic theory of plates, referred to as yield line theory, involves large rigid parts of the plate mutually rotating about yielding hinge lines, however in order to accommodate in plane plastic deformations area “collapse” yield lines have been introduced. The hinge...... yield lines accommodate differential rotations of rigid parts and the area “collapse” yield lines accommodate local area changes of the rigid parts thereby preserving compatibility of the rigid parts of a plate. The approach will be illustrated for rigid plastic column analysis and for a quadratic plate...

  4. Buckling analysis of thick isotropic plates by using exponential shear deformation theory

    Directory of Open Access Journals (Sweden)

    Sayyad A. S.

    2012-12-01

    Full Text Available In this paper, an exponential shear deformation theory is presented for the buckling analysis of thick isotropic plates subjected to uniaxial and biaxial in-plane forces. The theory accounts for a parabolic distribution of the transverse shear strains across the thickness, and satisfies the zero traction boundary conditions on the top and bottom surfaces of the plate without using shear correction factors. Governing equations and associated boundary conditions of the theory are obtained using the principle of virtual work. The simply supported thick isotropic square plates are considered for the detailed numerical studies. A closed form solutions for buckling analysis of square plates are obtained. Comparison studies are performed to verify the validity of the present results. The effects of aspect ratio on the critical buckling load of isotropic plates is investigated and discussed.

  5. Analysis of Rayleigh-Taylor Instability Part I: Bubble and Spike Count

    Energy Technology Data Exchange (ETDEWEB)

    Kamath, C; Gezahegne, A; Miller, P

    2006-08-08

    The use of high-performance computers to simulate hydrodynamic instabilities has resulted in the generation of massive amounts of data. One aspect of the analysis of this data involves the identification and characterization of coherent structures known as ''bubbles'' and ''spikes''. This can be a challenge as there is no precise definition of these structures, and the large size of the data, as well as its distributed nature, precludes any extensive experimentation with different definitions and analysis algorithms. In this report, we describe the use of image processing techniques to identify and count bubbles and spikes in the Rayleigh-Taylor instability, which occurs when an initially perturbed interface between a heavier fluid and a lighter fluid is allowed to grow under the influence of gravity. We analyze data from two simulations, one a large-eddy simulation with 30 terabytes of analysis data, and the other a direct numerical simulation with 80 terabytes of analysis data. We consider different techniques to first convert the three-dimensional data to two dimensions and then count the structures of interest in the two-dimensional data. Our analysis of the bubble and spike counts over time indicates that there are four distinct regimes in the process of the mixing of the two fluids, starting from the initial linear stage, followed by the non-linear stage with weak turbulence, the mixing transition stage, and the final stage of strong turbulence. We also show that our results are relatively insensitive to the parameters used in our algorithms.

  6. 桩帽设计中的厚板分析%THICK PLATE ANALYSIS WITH APPLICATION TO PILE CAP DESIGN

    Institute of Scientific and Technical Information of China (English)

    郑榕明; 张勇慧

    2004-01-01

    The thick plate is commonly used for the pile cap and the transfer plate, and a proper analysis of it can greatly affect the cost of the thick plate. In actual practice, soft support condition is sometimes adopted by engineers for modeling of thick plates. In this paper, the limitations of soft support in the determination of deflection and bending moment with very thick plates are studied. The distribution of superstructure loadings to pile caps and piles is also carefully investigated.

  7. Spectrum library concept and pulse shape analysis in liquid scintillation counting

    Energy Technology Data Exchange (ETDEWEB)

    Kaihola, L. [Wallac Oy, Turku (Finland)

    1997-03-01

    Wallac introduced in 1990 a new absolute liquid scintillation counting (LSC) method, Digital Overlay Technique (DOT) to correct for quench. This method allows quantization of multilabel samples by referring to library spectra which are generated against chemical and color quench indices at the factory. The libraries can further be expanded to any beta emitter by user with a method called fine tuning, which can be carried out even with a single sample. Spectrum libraries are created over the whole spectrum range of the radionuclide and allow automatic identification of a single label beta emitting radionuclide, called Easy Count method. Another improvement in LSC is commercial introduction of Pulse Shape Analysis (PSA) in 1986 by Wallac. This method recognizes alpha particle decay by pulse shape and leads to excellent sensitivity in alpha counting because most of the background signal in LSC comprises of short or beta like pulses. PSA detects alpha events in the presence of high excess of beta activity over alphas, up to a ratio 100000 to 1. (orig.)

  8. Application of Petrifilm Colony Count Plate in Microbial Detection for Cosmetics%大肠菌群测试片在化妆品微生物检测中的应用

    Institute of Scientific and Technical Information of China (English)

    朱世真; 王立芳; 王尊文

    2014-01-01

    目的:研究评价大肠菌群测试片( Petrifilm)在化妆品微生物检测中的应用。方法:通过对14批化妆品人工添加3种常见试验菌,采用《化妆品卫生规范》和Petrifilm菌落总数测试方法进行比对。结果:用两种方法检测结果差异不明显,经t检验,P>0.05,统计学数据表明两种方法相比差异无统计学意义。结论: Petrifilm菌落总数测试片法因其轻便、快速、简易的优势在化妆品的微生物检测中具有重要意义。%Objective:To study the application of petrifilm colony count plate in the microbial detection for cosmetics. Methods:Three common microbials in 14 batches of cosmetics were respectively detected by the method described in hygienic standard for cos-metics and petrifilm colony count plate, and the results were compared. Results:The results shown by the two methods had no statisti-cally significant difference (P>0. 05). Conclusion: Petrifilm colony count plate with light weight, fast and easy operation exhibits significance in the microbial detection for cosmetics.

  9. Analysis and design of rolling-contact joints for evaluating bone plate performance.

    Science.gov (United States)

    Slocum, Alexander H; Cervantes, Thomas M; Seldin, Edward B; Varanasi, Kripa K

    2012-09-01

    An apparatus for testing maxillofacial bone plates has been designed using a rolling contact joint. First, a free-body representation of the fracture fixation techniques utilizing bone plates is used to illustrate how rolling contact joints accurately simulate in vivo biomechanics. Next, a deterministic description of machine functional requirements is given, and is then used to drive the subsequent selection and design of machine elements. Hertz contact stress and fatigue analysis for two elements are used to ensure that the machine will both withstand loads required to deform different plates, and maintain a high cycle lifetime for testing large numbers of plates. Additionally, clinically relevant deformations are presented to illustrate how stiffness is affected after a deformation is applied, and to highlight improvements made by the machine over current testing standards, which do not adequately re-create in vivo loading conditions. The machine performed as expected and allowed for analysis of bone plates in both deformed and un-deformed configurations to be conducted. Data for deformation experiments is presented to show that the rolling-contact testing machine leads to improved loading configurations, and thus a more accurate description of plate performance. A machine for evaluation of maxillofacial bone plates has been designed, manufactured, and used to accurately simulate in vivo loading conditions to more effectively evaluate the performance of both new and existing bone plates.

  10. Analysis of the covariance structure of digital ridge counts in the offspring of monozygotic twins.

    Science.gov (United States)

    Cantor, R M; Nance, W E; Eaves, L J; Winter, P M; Blanchard, M M

    1983-03-01

    Improved methods for analysis of covariance structures now permit the rigorous testing of multivariate genetic hypotheses. Using Jöreskog's Lisrel IV computer program we have conducted a confirmatory factor analysis of dermal ridge counts on the individual fingers of 509 offspring of 107 monozygotic twin pairs. Prior to the initiation of the model-fitting procedure, the sex-adjusted ridge counts for the offspring of male and female twins were partitioned by a multivariate nested analysis of variance yielding five 10 X 10 variance-covariance matrices containing a total of 275 distinctly observed parameters with which to estimate latent sources of genetic and environmental variation and test hypotheses about the factor structure of those latent causes. To provide an adequate explanation for the observed patterns of covariation, it was necessary to include additive genetic, random environmental, epistatic and maternal effects in the model and a structure for the additive genetic effects which included a general factor and allowed for hand asymmetry and finger symmetry. The results illustrate the value of these methods for the analysis of interrelated metric traits.

  11. An automated image analysis system to measure and count organisms in laboratory microcosms.

    Directory of Open Access Journals (Sweden)

    François Mallard

    Full Text Available 1. Because of recent technological improvements in the way computer and digital camera perform, the potential use of imaging for contributing to the study of communities, populations or individuals in laboratory microcosms has risen enormously. However its limited use is due to difficulties in the automation of image analysis. 2. We present an accurate and flexible method of image analysis for detecting, counting and measuring moving particles on a fixed but heterogeneous substrate. This method has been specifically designed to follow individuals, or entire populations, in experimental laboratory microcosms. It can be used in other applications. 3. The method consists in comparing multiple pictures of the same experimental microcosm in order to generate an image of the fixed background. This background is then used to extract, measure and count the moving organisms, leaving out the fixed background and the motionless or dead individuals. 4. We provide different examples (springtails, ants, nematodes, daphnia to show that this non intrusive method is efficient at detecting organisms under a wide variety of conditions even on faintly contrasted and heterogeneous substrates. 5. The repeatability and reliability of this method has been assessed using experimental populations of the Collembola Folsomia candida. 6. We present an ImageJ plugin to automate the analysis of digital pictures of laboratory microcosms. The plugin automates the successive steps of the analysis and recursively analyses multiple sets of images, rapidly producing measurements from a large number of replicated microcosms.

  12. Finite strip method combined with other numerical methods for the analysis of plates

    Science.gov (United States)

    Cheung, M. S.; Li, Wenchang

    1992-09-01

    Finite plate strips are combined with finite elements or boundary elements in the analysis of rectangular plates with some minor irregularities such as openings, skew edges, etc. The plate is divided into regular and irregular regions. The regular region is analyzed by the finite strip method while the irregular one is analyzed by the finite element or boundary element method. A special transition element and strip are developed in order to connect the both regions. Numerical examples will show the accuracy and efficiency of this combined analysis.

  13. Analysis of Shrinkage on Thick Plate Part using Genetic Algorithm

    Directory of Open Access Journals (Sweden)

    Najihah S.N.

    2016-01-01

    Full Text Available Injection moulding is the most widely used processes in manufacturing plastic products. Since the quality of injection improves plastic parts are mostly influenced by process conditions, the method to determine the optimum process conditions becomes the key to improving the part quality. This paper presents a systematic methodology to analyse the shrinkage of the thick plate part during the injection moulding process. Genetic Algorithm (GA method was proposed to optimise the process parameters that would result in optimal solutions of optimisation goals. Using the GA, the shrinkage of the thick plate part was improved by 39.1% in parallel direction and 17.21% in the normal direction of melt flow.

  14. VOL AR PLATING OF DISTAL RADIUS FRACTURE : A RETROSPECTIVE ANALYSIS

    Directory of Open Access Journals (Sweden)

    Victor

    2015-06-01

    Full Text Available BACKGROUND: Treatment of distal radius has undergone remarkable changes since the time of Abraham colles. Conservative treatment with cast application has given way to operative techniques for better o utcome in high demand young individuals. Volar plating fo r distal end radius fractures is an effective technique which allows early mobilisation with re storation of r adial inclination , radial length , articular congruity and palmar tilt. MATERIALS AND METHODS: T his study was performed in chettinad hospital and research institute between January 2013 and M arch 2014. 24 patients with closed isolated distal end radius fractures treated with open reduction and internal fixation with plates and screws by volar approach were followed up retrospectively for a minimum period of one year . There were 17 men and 7 women. Mean age was 44 years (Range, 22 - 75 years. The fractures were classified based on the AO system. There were 4 A2, 12 B3, 5 C1, 3 C2 fracture types. RESULTS : All the patients were evaluated with standard anteroposterior and lateral radiographs and CT scans in c ase of intraarticular fractures . In all patie nts plating of distal end radius done by volar approach. Patients were followed postoperatively for one y ear radiologically and clinically by modified clinical scoring system by Green and O Brien. Five patients had excellent results, thirteen patients had good results with twenty five percent restriction of wrist function . Five patients had fair results. One patient had postop wound infection which required implant removal and external fixator application . CONCLUSION: With proper patient selection a nd accurate surgical techniques , volar plating continues to be a useful method of treatment for distal end radius fractures with minimal complications and allowing early return of patients to normal activities.

  15. Buckling Analysis of Functionally Graded Plates with Simply Supported Edges

    Directory of Open Access Journals (Sweden)

    Megueni ABDELKADER

    2009-12-01

    Full Text Available Thermal buckling analyses of S-FGM are investigated by using first order shear deformation theory. Material properties are varied continuously in the thickness direction according to a sigmoid distribution. The thermal buckling behaviours under uniform, linear and sinusoidal temperature rise across the thickness are analyzed. In addition, the effects of temperature field, volume fraction distributions, and system geometric parameters are investigated. The results are compared with the results of the classic plate theory (CPT.

  16. Vibration Analysis of Composite Rectangular Plates Reinforced along Curved Lines

    Science.gov (United States)

    Honda, Shinya; Oonishi, Yoshimasa; Narita, Yoshihiro; Sasaki, Katsuhiko

    In the past few decades, composite materials composed of straight fibers and polymer matrix have gained their status as the most promising material for light-weight structures. Technical merit of the composites as tailored material also provided practical advantages in the optimum design process. Recently, it is reported that the fabrication machine has been developed to make curved fibers embedded in the matrix material. Based on such technical advancement, this paper proposes an analytical method to study vibration of composite rectangular plates reinforced along curved lines. The approach is based on the Ritz method where variable fiber direction can be accommodated. For this purpose, the fibers continuously changing their direction are formulated as the variable bending stiffness in the total potential energy. A frequency equation is derived by the Ritz minimizing process, and frequency parameters are calculated as the eigenvlaues in the eigenvalue problem. In numerical results, the accuracy of the method is presented by comparing present results with FEM results. The advantages of present plate are confirmed by comparing natural frequencies and mode shapes with those of conventional composite and isotropic plates, and the effectiveness of the new solution to the most recent problem is demonstrated.

  17. A Hop-Count Analysis Scheme for Avoiding Wormhole Attacks in MANET.

    Science.gov (United States)

    Jen, Shang-Ming; Laih, Chi-Sung; Kuo, Wen-Chung

    2009-01-01

    MANET, due to the nature of wireless transmission, has more security issues compared to wired environments. A specific type of attack, the Wormhole attack does not require exploiting any nodes in the network and can interfere with the route establishment process. Instead of detecting wormholes from the role of administrators as in previous methods, we implement a new protocol, MHA, using a hop-count analysis from the viewpoint of users without any special environment assumptions. We also discuss previous works which require the role of administrator and their reliance on impractical assumptions, thus showing the advantages of MHA.

  18. A Hop-Count Analysis Scheme for Avoiding Wormhole Attacks in MANET

    Directory of Open Access Journals (Sweden)

    Chi-Sung Laih

    2009-06-01

    Full Text Available MANET, due to the nature of wireless transmission, has more security issues compared to wired environments. A specific type of attack, the Wormhole attack does not require exploiting any nodes in the network and can interfere with the route establishment process. Instead of detecting wormholes from the role of administrators as in previous methods, we implement a new protocol, MHA, using a hop-count analysis from the viewpoint of users without any special environment assumptions. We also discuss previous works which require the role of administrator and their reliance on impractical assumptions, thus showing the advantages of MHA.

  19. Analysis of Great World Wide Star Count Data: 2007-2013

    Science.gov (United States)

    Birriel, J. J.; Farrell, J. N.; Ward, D.

    2014-12-01

    The Great World Wide Star Count (GWWSC) website provides free public access to seven years of naked-eye limiting magnitudes (NELM) reported by citizen scientists from 2007 to 2013. We summarize the data and perform a simple statistical analysis. GWWSC data are compared with the Globe at Night (GaN) data over the same time period. The global average NELM values are generally comparable across the two data sets. Global NELM data seem to reflect shifts in urban versus suburban participation over time, while regional and local NELM data are more likely to reflect changes in night sky brightness.

  20. Two dimensional dynamic analysis of sandwich plates with gradient foam cores

    Energy Technology Data Exchange (ETDEWEB)

    Mu, Lin; Xiao, Deng Bao; Zhao, Guiping [State Key Laboratory for Mechanical structure Strength and Vibration, School of AerospaceXi' an Jiaotong University, Xi' an (China); Cho, Chong Du [Dept. of Mechanical Engineering, Inha University, Inchon (Korea, Republic of)

    2016-09-15

    Present investigation is concerned about dynamic response of composite sandwich plates with the functionally gradient foam cores under time-dependent impulse. The analysis is based on a model of the gradient sandwich plate, in which the face sheets and the core adopt the Kirchhoff theory and a [2, 1]-order theory, respectively. The material properties of the gradient foam core vary continuously along the thickness direction. The gradient plate model is validated with the finite element code ABAQUS®. And the results show that the proposed model can predict well the free vibration of composite sandwich plates with gradient foam cores. The influences of gradient foam cores on the natural frequency, deflection and energy absorbing of the sandwich plates are also investigated.

  1. Stress concentration analysis in functionally graded plates with elliptic holes under biaxial

    Directory of Open Access Journals (Sweden)

    Tawakol A. Enab

    2014-09-01

    Full Text Available Stress concentration factors (SCFs at the root of an elliptic hole in unidirectional functionally graded material (UDFGM plates under uniaxial and biaxial loads are predicted. ANSYS Parametric Design Language (APDL was used to build the finite element models for the plates and to run the analysis. A parametric study is performed for several geometric and material parameters such as the elliptic hole major axis to plate width ratio, the elliptical shape factor, the gradation direction of UDFGM. It is shown that, SCF in the finite plate can be significantly reduced by choosing the proper distribution of the functionally graded materials. The present study may provide designers an efficient way to estimate the hole effect on plate structures made of functionally graded materials.

  2. ANALYSIS ON THE MAGNETO-ELASTIC-PLASTIC BUCKLING/SNAPPING OF CANTILEVER RECTANGULAR FERROMAGNETIC PLATES

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    An analysis of buckling/snapping and bending behaviors of magneto-elastic-plastic interaction and coupling for cantilever rectangular soft ferromagnetic plates is presented. Based on the expression of magnetic force from the variational principle of ferromagnetic plates, the buckling and bending theory of thin plates, the Mises yield criterion and the increment theory for plastic deformation, we establish a numerical code to quantitatively simulate the behaviors of the nonlinearly multi-fields coupling problems by the finite element method. Along with the phenomena of buckling/snapping and bending, or the characteristic curve of deflection versus magnitude of applied magnetic fields being numerically displayed, the critical loads of buckling/snapping,and the influences of plastic deformation and the width of plate on these critical loads, the plastic regions expanding with the magnitude of applied magnetic field, as well as the evolvement of deflection configuration of the plate are numerically obtained in a case study.

  3. Design Evaluation Using Finite Element Analysis of Cooled Silicon Nitride Plates for a Turbine Blade Application

    Science.gov (United States)

    Abdul-Aziz, Ali; Baaklini, George Y.; Bhatt, Ramakrishna T.

    2001-01-01

    Two- and three-dimensional finite element analyses were performed on uncoated and thermal barrier coated (TBC) silicon nitride plates with and without internal cooling by air. Steady-state heat-transfer analyses were done to optimize the size and the geometry of the cooling channels to reduce thermal stresses, and to evaluate the thermal environment experienced by the plate during burner rig testing. The limited experimental data available were used to model the thermal profile exerted by the flame on the plate. Thermal stress analyses were performed to assess the stress response due to thermal loading. Contours for the temperature and the representative stresses for the plates were generated and presented for different cooling hole sizes and shapes. Analysis indicates that the TBC experienced higher stresses, and the temperature gradient was much reduced when the plate was internally cooled by air. The advantages and disadvantages of several cooling channel layouts were evaluated.

  4. Modelling Bending Wave Transmission across Coupled Plate Systems Comprising Periodic Ribbed Plates in the Low-, Mid-, and High-Frequency Ranges Using Forms of Statistical Energy Analysis

    Directory of Open Access Journals (Sweden)

    Jianfei Yin

    2015-01-01

    Full Text Available Prediction of bending wave transmission across systems of coupled plates which incorporate periodic ribbed plates is considered using Statistical Energy Analysis (SEA in the low- and mid-frequency ranges and Advanced SEA (ASEA in the high-frequency range. This paper investigates the crossover from prediction with SEA to ASEA through comparison with Finite Element Methods. Results from L-junctions confirm that this crossover occurs near the frequency band containing the fundamental bending mode of the individual bays on the ribbed plate when ribs are parallel to the junction line. Below this frequency band, SEA models treating each periodic ribbed plate as a single subsystem were shown to be appropriate. Above this frequency band, large reductions occur in the vibration level when propagation takes place across successive bays on ribbed plates when the ribs are parallel to the junction. This is due to spatial filtering; hence it is necessary to use ASEA which can incorporate indirect coupling associated with this transmission mechanism. A system of three coupled plates was also modelled which introduced flanking transmission. The results show that a wide frequency range can be covered by using both SEA and ASEA for systems of coupled plates where some or all of the plates are periodic ribbed plates.

  5. Liquid scintillation counting methodology for 99Tc analysis. A remedy for radiopharmaceutical waste

    Energy Technology Data Exchange (ETDEWEB)

    Khan, Mumtaz [Univ. of Science and Technology, Gyeongbuk (Korea); Um, Wooyong [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-08-13

    This paper presents a new approach for liquid scintillation counting (LSC) analysis of single-radionuclide samples containing appreciable organic or inorganic quench. This work offers better analytical results than existing LSC methods for technetium-99 (99gTc) analysis with significant savings in analysis cost and time. The method was developed to quantify 99gTc in environmental liquid and urine samples using LSC. Method efficiency was measured in the presence of 1.9 to 11,900 ppm total dissolved solids. The quench curve was proved to be effective in the case of spiked 99gTc activity calculation for deionized water, tap water, groundwater, seawater, and urine samples. Counting efficiency was found to be 91.66% for Ultima Gold LLT (ULG-LLT) and Ultima Gold (ULG). Relative error in spiked 99gTc samples was ±3.98% in ULG and ULG-LLT cocktails. Minimum detectable activity was determined to be 25.3 mBq and 22.7 mBq for ULG-LLT and ULG cocktails, respectively. A pre-concentration factor of 1000 was achieved at 100°C for 100% chemical recovery.

  6. Hierarchical models for failure analysis of plates bent by distributed and localized transverse loadings

    Institute of Scientific and Technical Information of China (English)

    Erasmo CARRERA; Gaetano GIUNTA

    2008-01-01

    The failure analysis of simply supported, isotropic, square plates is addressed. Attention focuses on minimum failure load amplitudes and failure locations, von Mises' equivalent stress along the plate thickness is also addressed. Several distributed and localized loading conditions are considered. Loads act on the top of the plate. Bi-sinusoidal and uniform loads are taken into account for distributed loadings, while stepwise constant centric and off-centric loadings are addressed in the case of localized loadings. Analysis is performed considering plates whose length-to-thickness ratio a/h can be as high as 100 (thin plates) and as low as 2 (very thick plates). Results are obtained via several 2D plate models. Classical theories (CTs) and higher order models are applied. Those theories are based on polynomial approximation of the displacement field. Among the higher order theories (HOTs), HOTsd models account for the transverse shear deformations, while HOTs models account for both transverse shear and transverse normal deformations. LHOTs represent a local application of the higher order theories. A layerwise approach is thus assumed: by means of mathematical interfaces, the plate is considered to be made of several fictitious layers. The exact 3D solution is presented in order to determine the accuracy of the results obtained via the 2D models. In this way a hierarchy among the 2D theories is established. CTs provide highly accurate results for a/h greater than 10 in the case of distributed loadings and greater than 20 for localized Ioadings. Results obtained via HOTs are highly accurate in the case of very thick plates for bi-sinusoidal and centric loadings. In the case of uniform and off-centric loadings a high gradient is present in the neighborhood of the plate top. In those cases, LHOTs yield results that match the exact solution.

  7. A Development of Force Plate for Biomechanics Analysis of Standing and Walking

    Science.gov (United States)

    Wardoyo, S.; Hutajulu, P. T.; Togibasa, O.

    2016-08-01

    Force plates are known as an excellent teaching aid to demonstrate the kinematics and dynamics of motion and commonly used in biomechanics laboratories to measure ground forces involved in the motion of human. It is consist of a metal plate with sensors attached to give an electrical output proportional to the force on the plate. Moreover, force plates are useful for examining the kinetic characteristics of an athlete's movement. They provide information about the external forces involved in movement that can aid a coach or sports scientist to quantitatively evaluate the athlete's skill development. In this study, we develop our prototype of force plate with less than 100,- simply by using flexible force transducer attached inside rubber matt, in the form of square blocks (dimension: 250 mm × 150 mm × 10 mm), with maximum load up to 60 kg. The handmade force plate was tested by applying biomechanics analysis for standing and walking. The testing was done on Experimental Soccer Courses’ students at the Department of Physical Education, Health and Recreation, University of Cenderawasih. The design of the force plate system together with biomechanics analysis will be discussed.

  8. A Delayed Neutron Counting System for the Analysis of Special Nuclear Materials

    Science.gov (United States)

    Sellers, Madison Theresa

    Nuclear forensic analysis is a modem science that uses numerous analytical techniques to identify and attribute nuclear materials in the event of a nuclear explosion, radiological terrorist attack or the interception of illicit nuclear material smuggling. The Canadian Department of National Defence has participated in recent international exercises that have highlighted the Nation's requirement to develop nuclear forensics expertise, protocol and capabilities, specifically pertaining to the analysis of special nuclear materials (SNM). A delayed neutron counting (DNC) system has been designed and established at the Royal Military College of Canada (RMC) to enhance the Government's SNM analysis capabilities. This analytical technique complements those already at RMC by providing a rapid and non-destructive method for the analysis of the fissile isotopes of both uranium (U) and plutonium (Pu). The SLOWPOKE-2 reactor at RMC produces a predominately thermal neutron flux. These neutrons induce fission in the SNM isotopes 233U, 235U and 239Pu releasing prompt fast neutrons, energy and radioactive fission fragments. Some of these fission fragments undergo beta - decay and subsequently emit neutrons, which can be recorded by an array of sensitive 3He detectors. The significant time period between the fission process and the release of these neutrons results in their identification as 'delayed neutrons'. The recorded neutron spectrum varies with time and the count rate curve is unique to each fissile isotope. In-house software, developed by this project, can analyze this delayed neutron curve and provides the fissile mass in the sample. Extensive characterization of the DNC system has been performed with natural U samples with 235 U content ranging from 2--7 microg. The system efficiency and dead time behaviour determined by the natural uranium sample analyses were validated by depleted uranium samples with similar quantities of 235 U resulting in a typical relative error of

  9. Source location in plates based on the multiple sensors array method and wavelet analysis

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Hong Jun; Shin, Tae Jin; Lee, Sang Kwon [Inha University, Incheon (Korea, Republic of)

    2014-01-15

    A new method for impact source localization in a plate is proposed based on the multiple signal classification (MUSIC) and wavelet analysis. For source localization, the direction of arrival of the wave caused by an impact on a plate and the distance between impact position and sensor should be estimated. The direction of arrival can be estimated accurately using MUSIC method. The distance can be obtained by using the time delay of arrival and the group velocity of the Lamb wave in a plate. Time delay is experimentally estimated using the continuous wavelet transform for the wave. The elasto dynamic theory is used for the group velocity estimation.

  10. S-F graphic representation analysis of photoelectric facula focometer poroo-plate glass

    Science.gov (United States)

    Tong, Yilin; Han, Xuecai

    2016-10-01

    Optical system focal length is usually based on the magnification method with focal length measurement poroo-plate glass is used as base element measuring focal length of focometer. On the basis of using analysis of magnification method to measure the accuracy of optical lens focal length, an expression between the ruling span of poroo-plate glass and the focal length of measured optical system was deduced, an efficient method to work out S-F graph with AUTOCAD was developed, the selecting principle of focometer parameter was analyzed, and Applied examples for designing poroo-plate glass in S-F figure was obtained.

  11. Stress and Strain Analysis of Functionally Graded Rectangular Plate with Exponentially Varying Properties

    Directory of Open Access Journals (Sweden)

    Amin Hadi

    2013-01-01

    Full Text Available The bending of rectangular plate made of functionally graded material (FGM is investigated by using three-dimensional elasticity theory. The governing equations obtained here are solved with static analysis considering the types of plates, which properties varying exponentially along direction. The value of Poisson’s ratio has been taken as a constant. The influence of different functionally graded variation on the stress and displacement fields was studied through a numerical example. The exact solution shows that the graded material properties have significant effects on the mechanical behavior of the plate.

  12. Three-Dimensional Effects in the Plate Element Analysis of Stitched Textile Composites

    Science.gov (United States)

    Glaessgen, E. H.; Raju, I. S.

    2000-01-01

    Three-dimensional effects related to the analysis of stitched textile composites are discussed. The method of calculation is based on the virtual crack closure technique (VCCT), and models that model the upper and lower surface of the delamination or debond with two-dimensional (2D) plate elements rather than three-dimensional (3D) solid elements. The major advantages of the plate element modeling technique are a smaller model size and simpler geometric modeling. Details of the modeling of the laminated plate and the stitching are discussed.

  13. Finite strip analysis of anisotropic laminated composite plates using higher-order shear deformation theory

    Science.gov (United States)

    Akhras, G.; Cheung, M. S.; Li, W.

    1994-08-01

    In the present study, a finite strip method for the elastic analysis of anisotropic laminated composite plates is developed according to higher-order shear deformation theory. This theory accounts for the parabolic distribution of the transverse shear strains through the thickness of the plate and for zero transverse shear stresses on the plate surfaces. In comparison with the finite strip method based on first-order shear deformation theory, the present method gives improved results while using approximately the same number of degrees of freedom. It also eliminates the need for shear correction factors in calculating the transverse shear stiffness.

  14. Modelling and analysis of fringing and metal thickness effects in MEMS parallel plate capacitors

    Science.gov (United States)

    Shah, Kriyang; Singh, Jugdutt; Zayegh, Aladin

    2005-12-01

    This paper presents a detailed design and analysis of fringing and metal thickness effects in a Micro Electro Mechanical System (MEMS) parallel plate capacitor. MEMS capacitor is one of the widely deployed components into various applications such are pressure sensor, accelerometers, Voltage Controlled Oscillator's (VCO's) and other tuning circuits. The advantages of MEMS capacitor are miniaturisation, integration with optics, low power consumption and high quality factor for RF circuits. Parallel plate capacitor models found in literature are discussed and the best suitable model for MEMS capacitors is presented. From the equations presented it is found that fringing filed and metal thickness have logarithmic effects on capacitance and depend on width of parallel plates, distance between them and thickness of metal plates. From this analysis a precise model of a MEMS parallel plate capacitor is developed which incorporates the effects of fringing fields and metal thickness. A parallel plate MEMS capacitor has been implemented using Coventor design suite. Finite Element Method (FEM) analysis in Coventorware design suite has been performed to verify the accuracy of the proposed model for suitable range of dimensions for MEMS capacitor Simulations and analysis show that the error between the designed and the simulated values of MEMS capacitor is significantly reduced. Application of the modified model for computing capacitance of a combed device shows that the designed values greatly differ from simulated results noticeably from 1.0339pF to 1.3171pF in case of fringed devices.

  15. Design and analysis of a folded Fresnel Zone Plate antenna

    Science.gov (United States)

    Ji, Yu; Fujita, Masaharu

    1994-08-01

    Based on the Kirchhoff-Huygens diffraction theory, a simple analytical method of a planar folded Fresnel zone-plate (FZP), that is the case when a planar reflector is placed behind the zone plates, has been developed. According to the numerical calculation results, the design procedure of the FZP antenna has been presented, and its focusing characteristics and gain-optimized conditions have been discussed. The variations of the focal field distribution with the antenna parameters such as zone numbers, focal length and antenna diameter and the radiation power patterns of the FZP have been simulated numerically. To take a good balance of both receiving and transmitting antennas, at 60GHz operating frequency, the focal length should be designed as a half of the antenna diameter and the zone number should be from 10 to 15. The results in this work show that the folded FZP has good focal characteristics and off-axis performance, and its antenna gain can be optimized by the suitable antenna parameter design. The possibility of applying the folded FZP as a low cost and high gain antenna without strict manufacturing requirement for millimeter-wave communications has been shown.

  16. Design Optimization of Hydraulic Press Plate using Finite Element Analysis

    Directory of Open Access Journals (Sweden)

    Akshay Vaishnav

    2016-05-01

    Full Text Available Metal forming is a process which is done by deforming metal work pieces to the desired shape and size using pressing or hammering action. Hydraulic presses are being used for forming and pressing operations with wide range of capacities. Hydraulic press machine works under continuous impact load. Because of this continuous load, tensile and compressive stresses are experienced in various parts of machine. These stresses cause permanent deformation in some parts of machine. This work is based on optimization of a 250-ton four pillar type hydraulic press considering constraints like design, weight and cost. The work is focused on design and optimization of top plate of the press machine. Top plate holds the hydraulic cylinder and is one of the most critical parts of the machine. The design is based on sizing optimization method and the results are validated by Finite Element method with proper boundary conditions. The CAD modelling has been carried out by PTC CREO and for FEA, ANSYS software is used.

  17. Can Detectability Analysis Improve the Utility of Point Counts for Temperate Forest Raptors?

    Science.gov (United States)

    Temperate forest breeding raptors are poorly represented in typical point count surveys because these birds are cryptic and typically breed at low densities. In recent years, many new methods for estimating detectability during point counts have been developed, including distanc...

  18. Microstructural analysis of MTR fuel plates damaged by a coolant flow blockage

    Science.gov (United States)

    Leenaers, A.; Joppen, F.; Van den Berghe, S.

    2009-10-01

    In 1975, as a result of a blockage of the coolant inlet flow, two plates of a fuel element of the BR2 reactor of the Belgian Nuclear Research Centre (SCK•CEN) were partially melted. The fuel element consisted of Al-clad plates with 90% 235U enriched UAl x fuel dispersed in an Al matrix. The element had accumulated a burn up of 21% 235U before it was removed from the reactor. Recently, the damaged fuel plates were sent to the hot laboratory for detailed PIE. Microstructural changes and associated temperature markers were used to identify several stages in the progression to fuel melting. It was found that the temperature in the center of the fuel plate had increased above 900-950 °C before the reactor was scrammed. In view of the limited availability of such datasets, the results of this microstructural analysis provide valuable input in the analysis of accident scenarios for research reactors.

  19. Comparison of multinomial and binomial proportion methods for analysis of multinomial count data.

    Science.gov (United States)

    Galyean, M L; Wester, D B

    2010-10-01

    Simulation methods were used to generate 1,000 experiments, each with 3 treatments and 10 experimental units/treatment, in completely randomized (CRD) and randomized complete block designs. Data were counts in 3 ordered or 4 nominal categories from multinomial distributions. For the 3-category analyses, category probabilities were 0.6, 0.3, and 0.1, respectively, for 2 of the treatments, and 0.5, 0.35, and 0.15 for the third treatment. In the 4-category analysis (CRD only), probabilities were 0.3, 0.3, 0.2, and 0.2 for treatments 1 and 2 vs. 0.4, 0.4, 0.1, and 0.1 for treatment 3. The 3-category data were analyzed with generalized linear mixed models as an ordered multinomial distribution with a cumulative logit link or by regrouping the data (e.g., counts in 1 category/sum of counts in all categories), followed by analysis of single categories as binomial proportions. Similarly, the 4-category data were analyzed as a nominal multinomial distribution with a glogit link or by grouping data as binomial proportions. For the 3-category CRD analyses, empirically determined type I error rates based on pair-wise comparisons (F- and Wald chi(2) tests) did not differ between multinomial and individual binomial category analyses with 10 (P = 0.38 to 0.60) or 50 (P = 0.19 to 0.67) sampling units/experimental unit. When analyzed as binomial proportions, power estimates varied among categories, with analysis of the category with the greatest counts yielding power similar to the multinomial analysis. Agreement between methods (percentage of experiments with the same results for the overall test for treatment effects) varied considerably among categories analyzed and sampling unit scenarios for the 3-category CRD analyses. Power (F-test) was 24.3, 49.1, 66.9, 83.5, 86.8, and 99.7% for 10, 20, 30, 40, 50, and 100 sampling units/experimental unit for the 3-category multinomial CRD analyses. Results with randomized complete block design simulations were similar to those with the CRD

  20. Thermal Stress Analysis of Laminated Composite Plates using Shear Flexible Element

    Directory of Open Access Journals (Sweden)

    M. Ganapathi

    1996-01-01

    Full Text Available Using degree Centigrade shear flexible QUAD-9 plate element, stresses and deflections in composite laminated plates due to thermal loads analysed. A formulation based on first order shear deformation theory has been employed for the analysis. The effects of various parameters, such as ply-angle, number of layers, thickness and aspect ratios on stresses and deflections are brought out. The present formulation is being extended for studying composite shell structures.

  1. Specific On-Plate Enrichment of Phosphorylated Peptides for Direct MALDI-TOF MS Analysis

    OpenAIRE

    Qiao, L.; Roussel, C.; Wan, J.; P. Yang; Girault, H H; Liu, B.

    2007-01-01

    An on-plate specific enrichment method is presented for the direct analysis of peptides phosphorylation. An array of sintered TiO2 nanoparticle spots was prepared on a stainless steel plate to provide porous substrate with a very large specific surface and durable functions. These spots were used to selectively capture phosphorylated peptides from peptide mixtures, and the immobilized phosphopeptides could then be analyzed directly by MALDI MS after washing away the nonphos- phorylated pepti...

  2. Influence of counting chamber type on CASA outcomes of equine semen analysis.

    Science.gov (United States)

    Hoogewijs, M K; de Vliegher, S P; Govaere, J L; de Schauwer, C; de Kruif, A; van Soom, A

    2012-09-01

    Sperm motility is considered to be one of the key features of semen analysis. Assessment of motility is frequently performed using computer-assisted sperm analysis (CASA). Nevertheless, no uniform standards are present to analyse a semen sample using CASA. We hypothesised that the type of counting chamber used might influence the results of analysis and aimed to study the effect of chamber type on estimated concentration and motility of an equine semen sample assessed using CASA. Commonly used disposable Leja chambers of different depths were compared with disposable and reusable ISAS chambers, a Makler chamber and a World Health Organization (WHO) motility slide. Motility parameters and concentrations obtained with CASA using these different chambers were analysed. The NucleoCounter was used as gold standard for determining concentration. Concentration and motility parameters were significantly influenced by the chamber type used. Using the NucleoCounter as the gold standard for determining concentration, the correlation coefficients were low for all of the various chambers evaluated, with the exception of the 12 µm deep Leja chamber. Filling a chamber by capillary forces resulted in a lower observed concentration and reduced motility parameters. All chambers evaluated in this study resulted in significant lower progressive motility than the WHO prepared slide, with the exception of the Makler chamber, which resulted in a slight, but statistically significant, increase in progressive motility estimates. Computer-assisted sperm analysis can only provide a rough estimate of sperm concentration and overestimation is likely when drop-filled slides with a coverslip are used. Motility estimates using CASA are highly influenced by the counting chamber; therefore, a complete description of the chamber type used should be provided in semen reports and in scientific articles. © 2011 EVJ Ltd.

  3. CAD-CAM plates versus conventional fixation plates for primary mandibular reconstruction: A biomechanical in vitro analysis.

    Science.gov (United States)

    Rendenbach, Carsten; Sellenschloh, Kay; Gerbig, Lucca; Morlock, Michael M; Beck-Broichsitter, Benedicta; Smeets, Ralf; Heiland, Max; Huber, Gerd; Hanken, Henning

    2017-09-01

    CAD/CAM reconstruction plates have become a viable option for mandible reconstruction. The aim of this study was to determine whether CAD/CAM plates provide higher fatigue strength compared with conventional fixation systems. 1.0 mm miniplates, 2.0 mm conventional locking plates (DePuy Synthes, Umkirch, Germany), and 2.0 mm CAD/CAM plates (Materialise, Leuven, Belgium/DePuy Synthes) were used to reconstruct a polyurethane mandible model (Synbone, Malans, CH) with cortical and cancellous bone equivalents. Mastication was simulated via cyclic dynamic testing using a universal testing machine (MTS, Bionix, Eden Prairie, MN, USA) until material failure reached a rate of 1 Hz with increasing loads on the left side. No significant difference was found between the groups until a load of 300 N. At higher loads, vertical displacement differed increasingly, with a poorer performance of miniplates (p = 0.04). Plate breakage occurred in miniplates and conventional locking plates. Screw breakage was recorded as the primary failure mechanism in CAD/CAM plates. Stiffness was significantly higher with the CAD/CAM plates (p = 0.04). CAD/CAM plates and reconstruction plates provide higher fatigue strength than miniplates, and stiffness is highest in CAD/CAM systems. All tested fixation methods seem sufficiently stable for mandible reconstruction. Copyright © 2017 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

  4. The Cognitive Advantages of Counting Specifically: A Representational Analysis of Verbal Numeration Systems in Oceanic Languages.

    Science.gov (United States)

    Bender, Andrea; Schlimm, Dirk; Beller, Sieghard

    2015-10-01

    The domain of numbers provides a paradigmatic case for investigating interactions of culture, language, and cognition: Numerical competencies are considered a core domain of knowledge, and yet the development of specifically human abilities presupposes cultural and linguistic input by way of counting sequences. These sequences constitute systems with distinct structural properties, the cross-linguistic variability of which has implications for number representation and processing. Such representational effects are scrutinized for two types of verbal numeration systems-general and object-specific ones-that were in parallel use in several Oceanic languages (English with its general system is included for comparison). The analysis indicates that the object-specific systems outperform the general systems with respect to counting and mental arithmetic, largely due to their regular and more compact representation. What these findings reveal on cognitive diversity, how the conjectures involved speak to more general issues in cognitive science, and how the approach taken here might help to bridge the gap between anthropology and other cognitive sciences is discussed in the conclusion.

  5. Histopathological analysis of apoptotic cell count and its role in oral lichen planus

    Directory of Open Access Journals (Sweden)

    Vidya G Doddawad

    2014-01-01

    Full Text Available Apoptosis is a process of genetically programmed cell death by which senescent, DNA-damaged and diseased cells are eliminated from the body. Aim of the Study: To identify and count the number of apoptotic cells in oral lichen planus (OLP and correlate with the degree of keratinization, thickness of epithelium and thickness of lymphocytic infiltration of OLP. Materials and Methods: The study comprised 40 diagnosed cases of OLP. Sections were stained with hematoxylin and eosin to identify and count the number of apoptotic cells. Measurement of other histopathological parameter of OLP such as degree of keratinization, thickness of epithelium and thickness of lymphocytic infiltration was done by using stage micrometer and eyepiece graticule. Statistical analysis was done to understand the correlation between apoptotic cells and histopathological features of OLP. Result: The result showed that the number of apoptotic cells increased, with an increase in thickness of lymphocytic infiltration and degree of keratinization, but there was a decrease in the epithelial thickness. Conclusion: Further immunological and molecular studies are required for a stronger evidence in correlating apoptotic cell and histological parameters of OLP.

  6. Abacus: a computational tool for extracting and pre-processing spectral count data for label-free quantitative proteomic analysis.

    Science.gov (United States)

    Fermin, Damian; Basrur, Venkatesha; Yocum, Anastasia K; Nesvizhskii, Alexey I

    2011-04-01

    We describe Abacus, a computational tool for extracting spectral counts from MS/MS data sets. The program aggregates data from multiple experiments, adjusts spectral counts to accurately account for peptides shared across multiple proteins, and performs common normalization steps. It can also output the spectral count data at the gene level, thus simplifying the integration and comparison between gene and protein expression data. Abacus is compatible with the widely used Trans-Proteomic Pipeline suite of tools and comes with a graphical user interface making it easy to interact with the program. The main aim of Abacus is to streamline the analysis of spectral count data by providing an automated, easy to use solution for extracting this information from proteomic data sets for subsequent, more sophisticated statistical analysis.

  7. Stress Analysis of Lap Joints With Embedded Cover Plate

    Directory of Open Access Journals (Sweden)

    İşcan Bahattin

    2015-06-01

    Full Text Available In this study, the effects of the bonding joints that are partly embedded in the adherent on the tensile behaviour of reinforcing plate adhesive joint have been investigated by 2D finite element method. In the study, the SBT 9244 material was used as the adhesive, and the adherent was the AA2024-T3 material. Three different models were built for the height of the embedded part in the adherent (a and five models for the length of the embedded part (b, while three models were built for the total overlap length (c. Results showed that with the increase of the height of the embedded part and total overlap length, the strength of the joint was considerably increased. The increase of the length of the embedded part was initially led to an increase in the strength of the joints but after 0.25 value of the b/c the strength was reduced.

  8. Supersonic flutter analysis of thin cracked functionally graded material plates

    CERN Document Server

    Natarajan, S; Bordas, S

    2012-01-01

    In this paper, the flutter behaviour of simply supported square functionally graded material plates immersed in a supersonic flow is studied. An enriched 4-noded quadrilateral element based on field consistency approach is used for this study and the crack is modelled independent of the underlying mesh. The material properties are assumed to be temperature dependent and graded only in the thickness direction. The effective material properties are estimated using the rule of mixtures. The formulation is based on the first order shear deformation theory and the shear correction factors are evaluated employing the energy equivalence principle. The influence of the crack length, the crack orientation, the flow angle and the gradient index on the aerodynamic pressure and the frequency are numerically studied. The results obtained here reveal that the critical frequency and the critical pressure decreases with increase in crack length and it is minimum when the crack is aligned to the flow angle.

  9. Morphology Analysis of Cu Film Fractures in Sandwiched Methylmethacrylate Plates

    Directory of Open Access Journals (Sweden)

    Cristiano Fidani

    2015-06-01

    Full Text Available Thin films of Cu were evaporated on solid plates of polymethylmethacrylate (PMMA. A polymerization process was made to realize sandwiched structure to protect the Cu films. Fracturing of the metal film surface was observed with several morphologies showing two different fracture systems. Surface film morphology was analysed in terms of the distribution area of the islands and contour fractal dimension. The island areas showed a maximum corresponding to 42 nm of the Cu thickness, it was also the threshold to observe the second fracture system. The fractures pattern resulted to be scale invariant with fractal dimensions between 1.55 and 1.7. The minimum fractal dimension also occurred at the film thickness corresponding to the maximum island area. The reported effects can be understood on the basis of different thermal expansion coefficients of the two materials and their thermally induced adhesion.DOI: http://dx.doi.org/10.5755/j01.ms.21.2.6518

  10. The Estimation of Vibrational Energy of Two Coupled (Welded Plates Using Statistical Energy Analysis

    Directory of Open Access Journals (Sweden)

    Hawraa S. Ibraham

    2011-01-01

    Full Text Available This paper deals with a method called Statistical Energy Analysis that can be applied to the mechanical and acoustical systems like buildings, bridges and aircrafts …etc. S.E.A as a tool can be applied to the resonant systems in the circumstances of high frequency or/and complex structure». The parameters of S.E.A such as coupling loss factor, internal loss factor, modal density and input power are clarified in this work ; coupled plate sub-systems and explanations are presented for these parameters. The developed system is assumed to be resonant, conservative, linear and there is an equipartition of energy between all the resonant modes within a given frequency band in a given sub-system. The aim of this work is to find the energy stored in the sub-systems for two coupled (welded plates in rectangular angle systems and study the effect of changing sub-systems dimensions, the results shows that as surface area of directly driven plates A1 increases energy level of plate 1 increases while a reduction in the energy level of indirectly driven plate (plate 2 is noticed. This is because of the strength of coupling decreases towards the weak coupling condition and this leads to a reduction in the power transferred from plate 1 to plate 2 and consequently a lower energy level for plate 2. In addition the effect of changing the internal loss factor for a range of (0.00001-0.1 causes a reduction of the values of energy level in these sub-systems. because the increasing of internal loss factor values led to the increasing of the material resistance and that will dissipate the energy flow across those sub-systems. A comparison is made between S.E.A models built by FORTRAN program and Finite Element model solved by ANSYS package.

  11. The Influence of Monitoring Interval on Data Measurement: An Analysis of Step Counts of University Students

    Directory of Open Access Journals (Sweden)

    Dagmar Sigmundová

    2013-01-01

    Full Text Available The pedometer is a widely used research tool for measuring the level and extent of physical activity (PA within population subgroups. The sample used in this study was drawn from a population of university students to examine the influence of the monitoring interval and alternate starting days on step-count activity patterns. The study was part of a national project during 2008–2010. Eligible subjects (641 were selected from a sample of 906 university students. The students wore pedometers continuously for 7 days excluding time for sleep and personal hygiene. Steps per day were logged on record sheets by each student. Data gathering spanned an entire week, and the results were sorted by alternate starting days, by activity for an entire week, by activity for only the weekdays of the one-week monitoring interval and for the two-day weekend. The statistical analysis included ANOVA, intra-class correlation (ICC analysis, and regression analysis. The ICC analysis suggested that monitoring starting on Monday (ICC = 0.71; 95%CI (0.61–0.79, Tuesday (ICC = 0.67; 95%CI (0.59–0.75 or Thursday (ICC = 0.68; 95%CI (0.55–0.79 improved reliability. The results of regression analysis also indicated that any starting day except Sunday is satisfactory as long as a minimum of four days of monitoring are used.

  12. Cryo-EM single particle analysis with the Volta phase plate.

    Science.gov (United States)

    Danev, Radostin; Baumeister, Wolfgang

    2016-01-01

    We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data. A double-area focusing strategy was implemented in order to achieve the required precision. With this approach we obtained a 3.2 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome. The phase plate matches or slightly exceeds the performance of the conventional defocus approach. Spherical aberration becomes a limiting factor for achieving resolutions below 3 Å with in-focus phase plate images. The phase plate could enable single particle analysis of challenging samples in terms of small size, heterogeneity and flexibility that are difficult to solve by the conventional defocus approach.

  13. Analysis of interlaminar stress and nonlinear dynamic response for composite laminated plates with interfacial damage

    Institute of Scientific and Technical Information of China (English)

    F. H. Zhu; Y. M. Fu

    2008-01-01

    By considering the effect of interfacial damage and using the variation principle, three-dimensional nonli-near dynamic governing equations of the laminated plates with interfacial damage are derived based on the general six-degrees-of-freedom plate theory towards the accurate stress analysis. The solutions of interlaminar stress and nonlinear dynamic response for a simply supported laminated plate with interfacial damage are obtained by using the finite dif-ference method, and the results are validated by compari-son with the solution of nonlinear finite element method. In numerical calculations, the effects of interfacial damage on the stress in the interface and the nonlinear dynamic response of laminated plates are discussed.

  14. Heat transfer and energy analysis of a solar air collector with smooth plate

    Science.gov (United States)

    Chabane, Foued; Moummi, Noureddine

    2014-04-01

    The heat transfer and thermal performance of a single pass solar air heater a smooth plate was investigated experimentally. In the present paper, energy and heat transfer analysis of a solar air collector with smooth plate, this technique is used to determine the optimal thermal performance of flat plate solar air heater by considering the different system and operating parameters to obtain maximum thermal performance. Thermal performance is obtained for different mass flow rate varying in the array 0.0108-0.0202 kg/s with five values, solar intensity; tilt angle and ambient temperature. We discuss the thermal behavior of this type of collector with new design and with my proper construction. An experimental study was carried out on a prototype installed on the experimental tests platform within the University of Biskra in the Algeria. The effects of air mass flow rate, emissivity of channel plates and wind heat transfer coefficient on the accuracy of the criterion are also investigated.

  15. Simplified Procedure For The Free Vibration Analysis Of Rectangular Plate Structures With Holes And Stiffeners

    Directory of Open Access Journals (Sweden)

    Cho Dae Seung

    2015-04-01

    Full Text Available Thin and thick plates, plates with holes, stiffened panels and stiffened panels with holes are primary structural members in almost all fields of engineering: civil, mechanical, aerospace, naval, ocean etc. In this paper, a simple and efficient procedure for the free vibration analysis of such elements is presented. It is based on the assumed mode method and can handle different plate thickness, various shapes and sizes of holes, different framing sizes and types as well as different combinations of boundary conditions. Natural frequencies and modes are determined by solving an eigenvalue problem of a multi-degree-of-freedom system matrix equation derived by using Lagrange’s equations. Mindlin theory is applied for a plate and Timoshenko beam theory for stiffeners. The applicability of the method in the design procedure is illustrated with several numerical examples obtained by the in-house developed code VAPS. Very good agreement with standard commercial finite element software is achieved.

  16. Analysis of the ORNL/TSF GCFR Grid-Plate Shield Design Confirmation Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Slater, C.O.; Cramer, S.N.; Ingersoll, D.T.

    1979-08-01

    The results of the analysis of the GCFR Grid-Plate Shield Design Confirmation Experiment are presented. The experiment, performed at the ORNL Tower Shielding Facility, was designed to test the adequacy of methods and data used in the analysis of the GCFR design. In particular, the experiment tested the adequacy of methods to calculate: (1) axial neutron streaming in the GCFR core and axial blanket, (2) the amount and location of the maximum fast-neutron exposure to the grid plate, and (3) the neutron source leaving the top of the grid plate and entering the upper plenum. Other objectives of the experiment were to verify the grid-plate shielding effectiveness and to assess the effects of fuel-pin and subassembly spacing on radiation levels in the GCFR. The experimental mockups contained regions representing the GCFR core/blanket region, the grid-plate shield section, and the grid plate. Most core design options were covered by allowing: (1) three different spacings between fuel subassemblies, (2) two different void fractions within a subassembly by variation of the number of fuel pins, and (3) a mockup of a control-rod channel.

  17. Dynamic Error Analysis Method for Vibration Shape Reconstruction of Smart FBG Plate Structure

    Directory of Open Access Journals (Sweden)

    Hesheng Zhang

    2016-01-01

    Full Text Available Shape reconstruction of aerospace plate structure is an important issue for safe operation of aerospace vehicles. One way to achieve such reconstruction is by constructing smart fiber Bragg grating (FBG plate structure with discrete distributed FBG sensor arrays using reconstruction algorithms in which error analysis of reconstruction algorithm is a key link. Considering that traditional error analysis methods can only deal with static data, a new dynamic data error analysis method are proposed based on LMS algorithm for shape reconstruction of smart FBG plate structure. Firstly, smart FBG structure and orthogonal curved network based reconstruction method is introduced. Then, a dynamic error analysis model is proposed for dynamic reconstruction error analysis. Thirdly, the parameter identification is done for the proposed dynamic error analysis model based on least mean square (LMS algorithm. Finally, an experimental verification platform is constructed and experimental dynamic reconstruction analysis is done. Experimental results show that the dynamic characteristics of the reconstruction performance for plate structure can be obtained accurately based on the proposed dynamic error analysis method. The proposed method can also be used for other data acquisition systems and data processing systems as a general error analysis method.

  18. Identifying time measurement tampering in the traversal time and hop count analysis (TTHCA) wormhole detection algorithm.

    Science.gov (United States)

    Karlsson, Jonny; Dooley, Laurence S; Pulkkis, Göran

    2013-05-17

    Traversal time and hop count analysis (TTHCA) is a recent wormhole detection algorithm for mobile ad hoc networks (MANET) which provides enhanced detection performance against all wormhole attack variants and network types. TTHCA involves each node measuring the processing time of routing packets during the route discovery process and then delivering the measurements to the source node. In a participation mode (PM) wormhole where malicious nodes appear in the routing tables as legitimate nodes, the time measurements can potentially be altered so preventing TTHCA from successfully detecting the wormhole. This paper analyses the prevailing conditions for time tampering attacks to succeed for PM wormholes, before introducing an extension to the TTHCA detection algorithm called ∆T Vector which is designed to identify time tampering, while preserving low false positive rates. Simulation results confirm that the ∆T Vector extension is able to effectively detect time tampering attacks, thereby providing an important security enhancement to the TTHCA algorithm.

  19. A New MANET wormhole detection algorithm based on traversal time and hop count analysis.

    Science.gov (United States)

    Karlsson, Jonny; Dooley, Laurence S; Pulkkis, Göran

    2011-01-01

    As demand increases for ubiquitous network facilities, infrastructure-less and self-configuring systems like Mobile Ad hoc Networks (MANET) are gaining popularity. MANET routing security however, is one of the most significant challenges to wide scale adoption, with wormhole attacks being an especially severe MANET routing threat. This is because wormholes are able to disrupt a major component of network traffic, while concomitantly being extremely difficult to detect. This paper introduces a new wormhole detection paradigm based upon Traversal Time and Hop Count Analysis (TTHCA), which in comparison to existing algorithms, consistently affords superior detection performance, allied with low false positive rates for all wormhole variants. Simulation results confirm that the TTHCA model exhibits robust wormhole route detection in various network scenarios, while incurring only a small network overhead. This feature makes TTHCA an attractive choice for MANET environments which generally comprise devices, such as wireless sensors, which possess a limited processing capability.

  20. Energy prices, technological knowledge and green energy innovation. A dynamic panel analysis of patent counts

    Energy Technology Data Exchange (ETDEWEB)

    Kruse, Juergen; Wetzel, Heike [Koeln Univ. (Germany). Dept. of Economics; Koeln Univ. (Germany). Energiewirtschaftliches Inst.

    2014-07-15

    We examine the effect of energy prices and technological knowledge on innovation in green energy technologies. In doing so, we consider both demand-pull effects, which induce innovative activity by increasing the expected value of innovations, and technology-push effects, which drive innovative activity by extending the technological capability of an economy. Our analysis is conducted using patent data from the European Patent Office on a panel of 26 OECD countries over the period 1978-2009. Utilizing a dynamic count data model for panel data, we analyze 11 distinct green energy technologies. Our results indicate that the existing knowledge stock is a significant driver of green energy innovation for all technologies. Furthermore, the results suggest that energy prices have a positive impact on innovation for some but not all technologies and that the e.ect of energy prices and technological knowledge on green energy innovation becomes more pronounced after the Kyoto protocol agreement in 1997.

  1. Identifying Time Measurement Tampering in the Traversal Time and Hop Count Analysis (TTHCA Wormhole Detection Algorithm

    Directory of Open Access Journals (Sweden)

    Jonny Karlsson

    2013-05-01

    Full Text Available Traversal time and hop count analysis (TTHCA is a recent wormhole detection algorithm for mobile ad hoc networks (MANET which provides enhanced detection performance against all wormhole attack variants and network types. TTHCA involves each node measuring the processing time of routing packets during the route discovery process and then delivering the measurements to the source node. In a participation mode (PM wormhole where malicious nodes appear in the routing tables as legitimate nodes, the time measurements can potentially be altered so preventing TTHCA from successfully detecting the wormhole. This paper analyses the prevailing conditions for time tampering attacks to succeed for PM wormholes, before introducing an extension to the TTHCA detection algorithm called ∆T Vector which is designed to identify time tampering, while preserving low false positive rates. Simulation results confirm that the ∆T Vector extension is able to effectively detect time tampering attacks, thereby providing an important security enhancement to the TTHCA algorithm.

  2. A New MANET Wormhole Detection Algorithm Based on Traversal Time and Hop Count Analysis

    Directory of Open Access Journals (Sweden)

    Göran Pulkkis

    2011-11-01

    Full Text Available As demand increases for ubiquitous network facilities, infrastructure-less and self-configuring systems like Mobile Ad hoc Networks (MANET are gaining popularity. MANET routing security however, is one of the most significant challenges to wide scale adoption, with wormhole attacks being an especially severe MANET routing threat. This is because wormholes are able to disrupt a major component of network traffic, while concomitantly being extremely difficult to detect. This paper introduces a new wormhole detection paradigm based upon Traversal Time and Hop Count Analysis (TTHCA, which in comparison to existing algorithms, consistently affords superior detection performance, allied with low false positive rates for all wormhole variants. Simulation results confirm that the TTHCA model exhibits robust wormhole route detection in various network scenarios, while incurring only a small network overhead. This feature makes TTHCA an attractive choice for MANET environments which generally comprise devices, such as wireless sensors, which possess a limited processing capability.

  3. A Comparative Analysis of Deterministic Sorting Algorithms based on Runtime and Count of Various Operations

    Directory of Open Access Journals (Sweden)

    Reyha Verma

    2015-12-01

    Full Text Available Sorting algorithms find its application in many practical fields of Computer Science. Efficient solving of sorting problem has attracted a great deal of research as it optimizes other algorithms also. The main factor which is taken into consideration while determining the efficiency of a sorting algorithm is the time complexity. Mostly the execution time of algorithms is investigated and compared for analyzing time complexity. This paper presents a comparative analysis of deterministic sorting algorithms. Time complexity of six different algorithms namely, Selection sort, Bubble sort, Insertion sort, Quicksort, Heapsort and Mergesort is determined in terms of number of comparisons, swaps and assignment operations in addition to average execution time. Also, the performance of these algorithms on fully and almost sorted lists was also analyzed. The study indicates that determining the operation’s count is essential for analyzing time complexity especially when algorithms are theoretically analyzed.

  4. Count-to-count time interval distribution analysis in a fast reactor; Estudio de la distribucion de intervalos de tiempo entre detecciones consecutivas de neutrones en un reactor rapido

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Navarro Gomez, A.

    1973-07-01

    The most important kinetic parameters have been measured at the zero power fast reactor CORAL-I by means of the reactor noise analysis in the time domain, using measurements of the count-to-count time intervals. (Author) 69 refs.

  5. 大肠菌群测试片中冷水可凝凝固剂初步研究%PRELIMINARY STUDY ON COLD-WATER-COAGULABLE COAGULATOR FOR COLIFORM COUNT PLATE

    Institute of Scientific and Technical Information of China (English)

    陈晓珑; 董煜; 陈灿映; 张晓峰; 李文杰

    2013-01-01

    In comparison with the tilt-pour method of the national standard, we studied the colloidal property of a self-made cold-water-coagulable coagulator (XGP) to evaluate the feasibility to serve as the coagulator of a coliform count plate, and also studied the water retention property, pH value, microorganism decomposition and utilization degree and antibacterial effect of XGP as well as the specificity, sensitivity and food sample detection rate after assembling to a count plate. The results showed that XGP was neutral, had no inhibition effect on 10 kinds of test strains, did not support microorganism growth in short time (5 days), and was difficult to be decomposed. In comparison with the tilt-pour method, the XGP had good water retention property (P0.05). Accordingly, the XGP had high specificity, high sensitivity, high economic property, and could be used in the coliform count plate as the medium carrier.%与国标倾注培养法比对,研究自制冷水可凝凝固剂(XGP)的胶体性能,评价其作为大肠菌群测试片凝固剂的可行性.对XGP的保水性能、pH值、微生物分解利用程度和抑菌效果进行研究,组装成测试片后进行特异性、灵敏度和食品样品检验.研究结果显示,XGP对10种试验菌株均无抑制作用,pH为中性,在短时间(5 d)内不支持微生物生长,不易被分解利用.与国标倾注培养法相比,其具有良好的保水性能(P<0.05),所检出的大肠菌群菌落数、灵敏度及食品样品阳性检出率差异均无统计学意义(P>0.05).XGP具有特异性强、灵敏度高及经济性好等优良特性,可作为培养基载体应用于大肠菌群测试片中.

  6. ATP生物发光法快速测定物体表面的菌落总数%Rapid determination of aerobic plate count on solid surfaces by ATP bioluminescent method

    Institute of Scientific and Technical Information of China (English)

    侯玉柱; 田雨; 柯润辉; 尹建军; 宋全厚

    2015-01-01

    In this paper Escherichia coli was used as an indicator to evaluate the surface hygiene by ATP bioluminescent method.We also simulated hygienic control from food factory to laboratory and determined the aerobic plate count of representative surface in laboratory by this method.Meanwhile,it was analyzed and compared with national standard microbiological examination.The results showed a good linear relationship between ATP fluorescence values and aerobic plate counts of E.coli,and their correlation coefficient R2 was 0.982 6.The designated laboratory surfaces were taken as blind samples.There was a good linear relationship between their ATP fluorescence values and aerobic plate counts,and their correlation coefficient R2 was 0.915 9.It illustrated that ATP bioluminescent method could assessed and determined accurately solid surface hygiene.%以大肠埃希氏菌作为指示菌考量了ATP生物发光法测定物体表面卫生状况的准确性,并在实验室内模拟食品企业卫生管控,采用ATP生物发光法对具有代表性的台面进行细菌总数测定,与国家标准微生物检测方法进行了比对分析.结果显示:大肠埃希氏菌的ATP荧光值与菌落总数的线性相关性良好,相关系数R2为0.982 6;以实验室内指定台面作为盲样进行对比测定,其ATP荧光值与菌落总数的线性相关性良好,相关系数R2达到0.915 9,说明ATP生物发光法能够准确评估和测定物体表面的卫生状况.

  7. High-Frequency Dynamic Analysis of Plates in Thermal Environments Based on Energy Finite Element Method

    Directory of Open Access Journals (Sweden)

    Di Wang

    2015-01-01

    Full Text Available The energy density governing equation to analyze the high-frequency dynamic behavior of plates in thermal environments is derived in this paper, in which the thermal effects are considered to change the membrane stress state and temperature dependent material properties of plates. Then the thermal effects on the energy reflection and transmission coefficients are dealt with hereof. Based on the above, an EFEM (energy finite element method based approximate approach for the energy analysis of coupled plates under nonuniform thermal environments is proposed. The approach could be conducted by three steps: (1 thermal analysis, (2 thermal stress analysis, and (3 forming element matrixes, joint matrixes, and the whole EFEM formulation for the energy analysis. The same mesh model is used for all the three steps. The comparison between EFEM results and classical modal superposition method results of simply supported plates in various uniform thermal environments and coupled plates in nonuniform thermal environments demonstrated that the derived energy governing equation and the proposed approach described well the smooth time- and locally space-averaged energy density. It is found that the distributions and levels of energy density are affected by thermal effects, and the variation trends are related to exciting frequency.

  8. Compression analysis of rectangular elastic layers bonded between rigid plates

    Energy Technology Data Exchange (ETDEWEB)

    Hsiang-Chuan Tsai [National Taiwan University of Science and Technology, Taipei (China). Dept. of Construction Engineering

    2005-06-01

    An elastic layer bonded between two rigid plates has higher compression stiffness than the elastic layer without bonding. While the finite element method can be applied to calculate the stiffness, the compression stiffness of bonded rectangular layers derived through a theoretical approach in this paper provides a convenient way for parametric study. Based on two kinematics assumptions, the governing equation for the mean pressure is derived from the equilibrium equations. Using the approximate shear boundary condition, the mean pressure is solved and the compression stiffness of the bonded rectangular layer is then established in an explicit single-series form. Through the solved pressure, the horizontal displacements are derived from the corresponding equilibrium equations, from which the shear stress on the bonding surface can be found. It is found that the effect of the rectangular aspect on the compression stiffness is significant only when Poisson's ratio is near 0.5. For the smaller Poisson's ratio, the compression stiffness of the rectangular layer can be approximated by the formula for the infinite-strip layer of the same shape factor. (author)

  9. Combined analysis of galaxy cluster number count, thermal Sunyaev-Zel'dovich power spectrum, and bispectrum

    Science.gov (United States)

    Hurier, G.; Lacasa, F.

    2017-08-01

    The thermal Sunyaev-Zel'dovich (tSZ) effect is a powerful probe of the evolution of structures in the universe, and is thus highly sensitive to cosmological parameters σ8 and Ωm, though its power is hampered by the current uncertainties on the cluster mass calibration. In this analysis we revisit constraints on these cosmological parameters as well as the hydrostatic mass bias, by performing (i) a robust estimation of the tSZ power-spectrum, (ii) a complete modeling and analysis of the tSZ bispectrum, and (iii) a combined analysis of galaxy clusters number count, tSZ power spectrum, and tSZ bispectrum. From this analysis, we derive as final constraints σ8 = 0.79 ± 0.02, Ωm = 0.29 ± 0.02, and (1-b) = 0.71 ± 0.07. These results favor a high value for the hydrostatic mass bias compared to numerical simulations and weak-lensing based estimations. They are furthermore consistent with both previous tSZ analyses, CMB derived cosmological parameters, and ancillary estimations of the hydrostatic mass bias.

  10. Validation of a leg movements count and periodic leg movements analysis in a custom polysomnography system.

    Science.gov (United States)

    Stefani, Ambra; Heidbreder, Anna; Hackner, Heinz; Högl, Birgit

    2017-02-23

    Periodic leg movements (PLM) during sleep (PLMS) are considered strongly related to restless legs syndrome (RLS), and are associated with polymorphisms in RLS risk genes. Various software for automatic analysis of PLMS are available, but only few of them have been validated. Aim of this study was to validate a leg movements count and analysis integrated in a commercially available polysomnography (PSG) system against manual scoring. Twenty RLS patients with a PLMS index > 20/h and 20 controls with a PLMS index PLM was performed according to the standard American Academy of Sleep Medicine (AASM) criteria. LM and PLM indices during sleep and wakefulness, the rate of PLMS associated with respiratory events, intermovement interval and periodicity indices were manually and automatically scored. The correlation between manual and computerized scoring was high for all investigated parameters (Spearman correlation coefficients 0.751-0.996, p PLM analysis against the gold standard manual scoring according to AASM criteria. The data demonstrate that the software used in this study has an outstanding performance for computerized LM and PLM scoring, and LM and PLM indices generated with this software can be reliably integrated in the routine PSG report. This automatic analysis is also an excellent tool for research purposes.

  11. Counting carbohydrates

    Science.gov (United States)

    Carb counting; Carbohydrate-controlled diet; Diabetic diet; Diabetes-counting carbohydrates ... Many foods contain carbohydrates (carbs), including: Fruit and fruit juice Cereal, bread, pasta, and rice Milk and milk products, soy milk Beans, legumes, ...

  12. Seal Counts

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Database of seal counts from aerial photography. Counts by image, site, species, and date are stored in the database along with information on entanglements and...

  13. Platelet Count

    Science.gov (United States)

    ... their spleen removed surgically Use of birth control pills (oral contraceptives) Some conditions may cause a temporary (transitory) increased ... increased platelet counts include estrogen and birth control pills (oral contraceptives). Mildly decreased platelet counts may be seen in ...

  14. MULTILAYERED PLATE ELEMENTS WITH NODE-DEPENDENT KINEMATICS FOR THE ANALYSIS OF COMPOSITE AND SANDWICH STRUCTURES

    Directory of Open Access Journals (Sweden)

    Stefano Valvano

    2017-04-01

    Full Text Available In this paper a new plate finite element (FE for the analysis of composite and sandwich plates is proposed. By making use of the node-variable plate theory assumptions, the new finite element allows for a simultaneous analysis of different subregions of the problem domain with different kinematics and accuracy, in a global/local sense. In particular higher-order theories with an Equivalent-Single-Layer (ESL approach are simultaneously used with advanced Layer-Wise (LW models. As a consequence, the computational costs can be reduced drastically by assuming refined theories only in those zones/nodes of the structural domain where the resulting strain and stress states present a complex distribution. On the contrary, computationally cheaper, low-order kinematic assumptions can be used in the remaining parts of the plate where a localized detailed analysis is not necessary. The primary advantage of the present variable-kinematics element and related global/local approach is that no ad-hoc techniques and mathematical artifices are required to mix the fields coming from two different and kinematically incompatible adjacent elements, because the plate structural theory varies within the finite element itself. In other words, the structural theory of the plate element is a property of the FE node in this present approach, and the continuity between two adjacent elements is ensured by adopting the same kinematics at the interface nodes. According to the Unified Formulation by Carrera, the through-the-thickness unknowns are described by Taylor polynomial expansions with ESL approach and by Legendre polynomials with LW approach. Furthermore, the Mixed Interpolated Tensorial Components (MITC method is employed to contrast the shear locking phenomenon. Several numerical investigations are carried out to validate and demonstrate the accuracy and efficiency of the present plate element, including comparison with various closed-form and FE solutions from the

  15. Geometrically nonlinear vibration analysis of piezoelectrically actuated FGM plate with an initial large deformation

    Energy Technology Data Exchange (ETDEWEB)

    Naei, Mohammad Hassan; Rastgoo, Abbas [University of Tehran, Tehran (Iran, Islamic Republic of); Ebrahimi, Farzad [Faculty of Engineering and Technology, lmam Khomeini International University, Qazvin (Iran, Islamic Republic of)

    2009-08-15

    A theoretical model for geometrically nonlinear vibration analysis of piezoelectrically actuated circular plates made of functionally grade material (FGM) is presented based on Kirchhoff's-Love hypothesis with von-Karman type geometrical large nonlinear deformations. To determine the initial stress state and pre-vibration deformations of the smart plate a nonlinear static problem is solved followed by adding an incremental dynamic state to the pre-vibration state. The derived governing equations of the structure are solved by exact series expansion method combined with perturbation approach. The material properties of the FGM core plate are assumed to be graded in the thickness direction according to the power-law distribution in terms of the volume fractions of the constituents. Control of the FGM plate's nonlinear deflections and natural frequencies using high control voltages is studied and their nonlinear effects are evaluated. Numerical results for FG plates with various mixture of ceramic and metal are presented in dimensionless forms. In a parametric study the emphasis is placed on investigating the effect of varying the applied actuator voltage as well as gradient index of FGM plate on vibration characteristics of the smart structure

  16. Microstructural Analysis of Irradiated U-Mo Fuel Plates: Recent Results

    Energy Technology Data Exchange (ETDEWEB)

    D. D. Keiser, Jr.; J. Jue; B. D. Miller; J. Gan; A. B. Robinson; P. V. Medvedev

    2012-03-01

    Microstructural characterization of irradiated dispersion and monolithic RERTR fuel plates using scanning electron microscopy (SEM) is being performed in the Electron Microscopy Laboratory at the Idaho National Laboratory. The SEM analysis of samples from U-Mo dispersion fuel plates focuses primarily on the behavior of the Si that has been added to the Al matrix to improve the irradiation performance of the fuel plate and on the overall behavior of fission gases (e.g., Xe and Kr) that develop as bubbles in the fuel microstructure. For monolithic fuel plates, microstructural features of interest, include those found in the U-Mo foil and at the U-Mo/Zr and Zr/6061 Al cladding interfaces. For both dispersion and monolithic fuel plates, samples have been produced using an SEM equipped with a Focused Ion Beam (FIB). These samples are of very high quality and can be used to uncover some very unique microstructural features that are typically not observed when characterizing samples produced using more conventional techniques. Overall, for the dispersion fuel plates with matrices that contained Si, narrower fuel/matrix interaction layers are typically observed compared to the fuel plates with pure Al matrix, and for the monolithic fuel plates microstructural features have been observed in the U-10Mo foil that are similar to what have been observed in the fuel particles found in U-Mo dispersion fuels. Most recently, more prototypic monolithic fuel samples have been characterized and this paper describes the microstructures that have been observed in these samples.

  17. MyPlate

    Science.gov (United States)

    ... our stage of life, situations, preferences, access to food, culture, traditions, and the personal decisions we make over time. All your food and beverage choices count. MyPlate offers ideas and ...

  18. Implementation and Initial Testing of Advanced Processing and Analysis Algorithms for Correlated Neutron Counting

    Energy Technology Data Exchange (ETDEWEB)

    Santi, Peter Angelo [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Cutler, Theresa Elizabeth [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Favalli, Andrea [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Koehler, Katrina Elizabeth [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Henzl, Vladimir [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Henzlova, Daniela [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Parker, Robert Francis [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Croft, Stephen [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-12-01

    In order to improve the accuracy and capabilities of neutron multiplicity counting, additional quantifiable information is needed in order to address the assumptions that are present in the point model. Extracting and utilizing higher order moments (Quads and Pents) from the neutron pulse train represents the most direct way of extracting additional information from the measurement data to allow for an improved determination of the physical properties of the item of interest. The extraction of higher order moments from a neutron pulse train required the development of advanced dead time correction algorithms which could correct for dead time effects in all of the measurement moments in a self-consistent manner. In addition, advanced analysis algorithms have been developed to address specific assumptions that are made within the current analysis model, namely that all neutrons are created at a single point within the item of interest, and that all neutrons that are produced within an item are created with the same energy distribution. This report will discuss the current status of implementation and initial testing of the advanced dead time correction and analysis algorithms that have been developed in an attempt to utilize higher order moments to improve the capabilities of correlated neutron measurement techniques.

  19. Sales of Forestry-Related Specialty License Plates in the Southern United States: A County Level Empirical Analysis

    Directory of Open Access Journals (Sweden)

    Shaun M. Tanger

    2016-07-01

    Full Text Available In recent years, specialty license plates have become an increasingly popular way to raise awareness and show support for a myriad of issues with which the plate is linked. Several states and various organizations that provide forestry education have developed forestry license plates. Vehicle owners can purchase the plates to show their support towards forestry by buying the forestry license plates, which generates revenue for the provider organization. Using county-level data from five states in the Southeastern United States, a statistical model was developed to examine explanatory factors of forestry-based specialty license plate sales in 2014. Using linear count regression modeling, we observed that the significant predictor variables of plate sales were income per capita, population density, the percentage of acres that are forested in the county, acres of forest in the county that are privately owned, percentage of people who are 65 or older, and presence of the forest industry in the county. Plate sales were positively correlated with the presence of the forest industry in the county.

  20. How to optimize the drop plate method for enumerating bacteria.

    Science.gov (United States)

    Herigstad, B; Hamilton, M; Heersink, J

    2001-03-01

    The drop plate (DP) method can be used to determine the number of viable suspended bacteria in a known beaker volume. The drop plate method has some advantages over the spread plate (SP) method. Less time and effort are required to dispense the drops onto an agar plate than to spread an equivalent total sample volume into the agar. By distributing the sample in drops, colony counting can be done faster and perhaps more accurately. Even though it has been present in the laboratory for many years, the drop plate method has not been standardized. Some technicians use 10-fold dilutions, others use twofold. Some technicians plate a total volume of 0.1 ml, others plate 0.2 ml. The optimal combination of such factors would be useful to know when performing the drop plate method. This investigation was conducted to determine (i) the standard deviation of the bacterial density estimate, (ii) the cost of performing the drop plate procedure, (iii) the optimal drop plate design, and (iv) the advantages of the drop plate method in comparison to the standard spread plate method. The optimal design is the combination of factor settings that achieves the smallest standard deviation for a fixed cost. Computer simulation techniques and regression analysis were used to express the standard deviation as a function of the beaker volume, dilution factor, and volume plated. The standard deviation expression is also applicable to the spread plate method.

  1. ANALYSIS OF SHAKEDOWN OF FG BREE PLATE SUBJECTED TO COUPLED THERMAL-MECHANICAL LOADINGS

    Institute of Scientific and Technical Information of China (English)

    Xianghe Peng; Ning Hu; Hengwei Zheng; Cuirong Fang

    2009-01-01

    The static and kinematic shakedown of a functionally graded (FG) Bree plate is analyzed. The plate is subjected to coupled constant mechanical load and cyclically varying tem-perature. The material is assumed linearly elastic and nonlinear isotropic hardening with elastic modulus, yield strength and the thermal expansion coefficient varying exponentially through the thickness of the plate. The boundaries between the shakedown area and the areas of elasticity, incremental collapse and reversed plasticity are determined, respectively. The shakedown of the counterpart made of homogeneous material with average material properties is also analyzed. The comparison between the results obtained in the two cases exhibits distinct qualitative and quantitative difference, indicating the importance of shakedown analysis for FG structures. Since FG structures are usually used in the cases where severe coupled cyclic thermal and mechani-cal loadings are applied, the approach developed and the results obtained are significant for the analysis and design of such kind of structures.

  2. Failure analysis of multiple delaminated composite plates due to bending and impact

    Indian Academy of Sciences (India)

    P K Parhi; S K Bhattacharyya; P K Sinha

    2001-04-01

    The present work aims at the first ply failure analysis of laminated composite plates with arbitrarily located multiple delaminations subjected to transverse static load as well as impact. The theoretical formulation is based on a simple multiple delamination model. Conventional first order shear deformation is assumed using eight-noded isoparametric quadratic elements to develop the finite element analysis procedure. Composite plates are assumed to contain both single and multiple delaminations. For the case of impact, Newmark time integration algorithm is employed for solving the time dependent multiple equations of the plate and the impactor. Tsai-Wu failure criterion is used to check for failure of the laminate for both the cases. To investigate the first ply failure, parametric studies are made for different cases by varying the size and number of delaminations as well as the stacking sequences and boundary conditions.

  3. Application of Crown-Flatness Vector Analysis in Plate Rolling Schedule

    Institute of Scientific and Technical Information of China (English)

    HU Xian-lei; WANG Zhao-dong; JIAO Zhi-jie; ZHAO Zhong; LIU Xiang-hua; WANG Guo-dong

    2004-01-01

    A simple plate crown model was introduced, and the crown-flatness vector analysis method was analyzed. Based on the plate rolling technology, the rolling schedule design of elongation phase is divided into three steps. First step is to calculate the reductions of first pass of elongation making full use of the mill capability to decrease the total pass number. The second step is to calculate the pass reduction for the last three or four passes to control crown and flatness by crown-flatness vector analysis method. In the third step, the maximum rolling force limit and the total pass number are adjusted to make the plate gauge at exit equal to target gauge with satisfactory flatness. The on-line application shows that this method is effective.

  4. Layerwise mechanics and finite element for the dynamic analysis of piezoelectric composite plates

    Science.gov (United States)

    Saravanos, Dimitris A.; Heyliger, Paul R.; Hopkins, Dale A.

    1996-01-01

    Laminate and structural mechanics for the analysis of laminated composite plate structures with piezoelectric actuators and sensors are presented. The theories implement layerwise representations of displacements and electric potential, and can model both the global and local electromechanical response of smart composite laminates. Finite-element formulations are developed for the quasi-static and dynamic analysis of smart composite structures containing piezoelectric layers. Comparisons with an exact solution illustrate the accuracy, robustness and capability of the developed mechanics to capture the global and local response of thin and/or thick laminated piezoelectric plates. Additional correlations and numerical applications demonstrate the unique capabilities of the mechanics in analyzing the static and free-vibration response of composite plates with distributed piezoelectric actuators and sensors.

  5. Analysis Of Drilling Of A Metal Plate With Pilot Hole

    Directory of Open Access Journals (Sweden)

    Kim K.S.

    2015-06-01

    Full Text Available This paper performed a numerical analysis study of the cutting characteristics when drilling a taper hole whose upper and lower diameters are different, that is, a pilot hole. 3-dimensional drilling analysis was performed using a commercial code, and these results were used to determine analytically and compare the cutting temperature, the phenomenon of cutting chip formation, deformation and pressure, etc., for the tools, chips, and specimens during cutting. Also, cutting force and the required energy were calculated and compared. To verify the results of analysis, a cutting dynamometer was used to analyze 3-axis cutting force. Results of analysis showed that the specimen maintained a temperature of approximately 650°, with rapid cooling after chips were formed from the specimen. The tool reached a maximum of 700°, similarly to the chips. It is expected that these results will be utilized to optimize cutting processes and work conditions when drilling holes.

  6. Biomechanical Property of a Newly Designed Assembly Locking Compression Plate: Three-Dimensional Finite Element Analysis

    Directory of Open Access Journals (Sweden)

    Jiang-Jun Zhou

    2017-01-01

    Full Text Available In this study, we developed and validated a refined three-dimensional finite element model of middle femoral comminuted fracture to compare the biomechanical stability after two kinds of plate fixation: a newly designed assembly locking compression plate (NALCP and a locking compression plate (LCP. CT data of a male volunteer was converted to middle femoral comminuted fracture finite element analysis model. The fracture was fixated by NALCP and LCP. Stress distributions were observed. Under slow walking load and torsion load, the stress distribution tendency of the two plates was roughly uniform. The anterolateral femur was the tension stress area, and the bone block shifted toward the anterolateral femur. Maximum stress was found on the lateral border of the number 5 countersink of the plate. Under a slow walking load, the NALCP maximum stress was 2.160e+03 MPa and the LCP was 8.561e+02 MPa. Under torsion load, the NALCP maximum stress was 2.260e+03 MPa and the LCP was 6.813e+02 MPa. Based on those results of finite element analysis, the NALCP can provide adequate mechanical stability for comminuted fractures, which would help fixate the bone block and promote bone healing.

  7. Automated screening for myelodysplastic syndromes through analysis of complete blood count and cell population data parameters.

    Science.gov (United States)

    Raess, Philipp W; van de Geijn, Gert-Jan M; Njo, Tjin L; Klop, Boudewijn; Sukhachev, Dmitry; Wertheim, Gerald; McAleer, Tom; Master, Stephen R; Bagg, Adam

    2014-04-01

    The diagnosis of myelodysplastic syndromes (MDS) requires a high clinical index of suspicion to prompt bone marrow studies as well as subjective assessment of dysplastic morphology. We sought to determine if data collected by automated hematology analyzers during complete blood count (CBC) analysis might help to identify MDS in a routine clinical setting. We collected CBC parameters (including those for research use only and cell population data) and demographic information in a large (>5,000), unselected sequential cohort of outpatients. The cohort was divided into independent training and test groups to develop and validate a random forest classifier that identifies MDS. The classifier effectively identified MDS and had a receiver operating characteristic area under the curve (AUC) of 0.942. Platelet distribution width and the standard deviation of red blood cell distribution width were the most discriminating variables within the classifier. Additionally, a similar classifier was validated with an additional, independent set of >200 patients from a second institution with an AUC of 0.93. This retrospective study demonstrates the feasibility of identifying MDS in an unselected outpatient population using data routinely collected during CBC analysis with a classifier that has been validated using two independent data sets from different institutions.

  8. Operational modal analysis of a rectangular plate using non-contact excitation and measurement

    Science.gov (United States)

    Xu, Y. F.; Zhu, W. D.

    2013-09-01

    Operational modal analysis (OMA), or output-only modal analysis, has been extensively developed in the past decades and widely used especially when the input is unknown and difficult to measure. This paper presents a non-contact experimental technique for measuring modal parameters of a rectangular aluminum plate with free boundaries using only the output data, with the intention to apply the technique to turbine blades. OMA is used to analyze both the out-of-plane and in-plane vibrations of the plate in the frequency range of up to 15,000 Hz, which can be an operation frequency range of a turbine blade, under white noise acoustic excitation in a direction of interest. It is shown that OMA can be performed when the types of measurement at the measurement and reference points are different, since the associated cross-correlation functions contain modal characteristics of the test structure. A single-point laser vibrometer and a free-field microphone are used to simultaneously measure the responses of the plate in a non-contact manner, with the microphone measurement serving as the reference. The pressure measured by the microphone near the reference point is shown to be proportional to the normal surface acceleration at the reference point, and the cross power spectral densities obtained by the current test method can be used for modal parameter estimation. A method for measuring the in-plane modes of the plate by shining the laser beam on the plate surface with an incident angle is developed. Experimental modal analysis (EMA) is also performed on the plate using an impact hammer and the laser vibrometer. The measured natural frequencies and mode shapes of the out-of-plane and in-plane modes of the plate using OMA and EMA are compared with those calculated using commercial finite element software. The maximum error between the measured and calculated natural frequencies of the plate is 1.53 percent for the first 18 elastic modes, including 16 out-of-plane and two in

  9. Analysis of the distribution of the brain cells of the fruit fly by an automatic cell counting algorithm

    Science.gov (United States)

    Shimada, Takashi; Kato, Kentaro; Kamikouchi, Azusa; Ito, Kei

    2005-05-01

    The fruit fly is the smallest brain-having model animal. Its brain is said to consist only of about 250,000 neurons, whereas it shows “the rudiments of consciousness” in addition to its high abilities such as learning and memory. As the starting point of the exhaustive analysis of its brain-circuit information, we have developed a new algorithm of counting cells automatically from source 2D/3D figures. In our algorithm, counting cells is realized by embedding objects (typically, disks/balls), each of which has exclusive volume. Using this method, we have succeeded in counting thousands of cells accurately. This method provides us the information necessary for the analysis of brain circuits: the precise distribution of the whole brain cells.

  10. Biomechanical analysis of titanium fixation plates and screws in ...

    African Journals Online (AJOL)

    2015-08-10

    Aug 10, 2015 ... complex because the major stress‑bearing of the mandible are disrupted in this ... elastic as were the other materials used in this analysis. The .... the computational model is developed based on the modular principle and is ...

  11. Early failure of bioabsorbable anterior cervical fusion plates: case report and failure analysis.

    Science.gov (United States)

    Brkaric, Mario; Baker, Kevin C; Israel, Raj; Harding, Trevor; Montgomery, David M; Herkowitz, Harry N

    2007-05-01

    Case report with forensic failure analysis. To determine the failure modes of 3 explanted 70:30 PLDLA Mystique (Medtronic Sofamor Danek, Memphis, TN) graft containment plates retrieved from revision surgery for early device failure. To reduce the problems of stress-shielding and radiopacity associated with metallic systems, bioabsorbable polymers have been used in anterior cervical discectomy and fusion procedures. Degradation of mechanical properties in vivo is a major concern when using bioabsorbable systems. Three of 6 patients who underwent anterior cervical discectomy with instrumented fusion, using Mystique graft containment systems experienced early failure requiring revision to alternate hardware. Devices were retrieved after failure and analyzed by light microscopy and environmental scanning electron microscopy. Simulations were performed with an unused plating system to induce damage for comparison with the retrieved devices. A detailed case review was performed to identify possible sources of extraordinary loading or damage. One plating system failed at 6 weeks postimplantation due to fatigue fracture of the screws. Crack initiation sites were identified at the interface of the thread root and mold line of the screw. Another plating system failed at 16 weeks postimplantation due to the coalescence of radial microcracking between holes in the plate, leading to catastrophic failure of the plate. The final plating system failed during the implantation surgery, when the screw fractured in torsion. Stress concentrations at the screw head-shaft interface and thread-shaft interface reduce the fatigue performance of bioabsorbable screws. Hydrolysis of the polymer may also play a role in the reduction of resistance to crack initiation and propagation.

  12. Titre plate and measuring device for chemical and/or biochemical analysis

    NARCIS (Netherlands)

    Bossche, A.; Kutchoukov, V.G.; Garini, Y.; Young, I.A.

    2006-01-01

    The invention relates to a titre plate for chemical and/or biochemical analysis based on an illumination measurement comprising a transparent substrate, applied to the substrate there is a metallic layer, which is provided with an array of reaction chambers, wherein the reaction chambers extend from

  13. THREE-DIMENSIONAL ANALYSIS OF FUNCTIONALLY GRADED PLATE BASED ON THE HAAR WAVELET METHOD

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A three-dimensional analysis of a simply-supported functionally graded rectangular plate with an arbitrary distribution of material properties is made using a simple and effective method based on the Haar wavelet. With good features in treating singularities, Haar series solution converges rapidly for arbitrary distributions, especially for the case where the material properties change rapidly in some regions. Through numerical examples the influences of the ratio of material constants on the top and bottom surfaces and different material gradient distributions on the structural response of the plate to mechanical stimuli are studied.

  14. Displacement analysis of a bend plate test with mechanical loading and laser heating

    Energy Technology Data Exchange (ETDEWEB)

    Lam, P.S.

    1997-09-01

    The surface displacment of a steel plate caused by a permanent deformation as a result of local yielding was modeled by a finite element analysis. The local yielding occurs when a small area of the plate is heated by a laser beam. The calculated displacments are in good agreement with the preliminary experimental data obtained using a bend specimen with laser heating at the University of Alabama at Huntsville. It has been shown computuationally and optically that the relative displacments are less than 1mm near the laser heated area of the specimen. The results demonstrate that the experimental approach is a feasible technique for determining the residual stress under multiaxial stress field.

  15. On prestress stiffness analysis of bolt-plate contact assemblies

    DEFF Research Database (Denmark)

    Pedersen, Niels Leergaard; Pedersen, Pauli

    2008-01-01

    Bolt connections are among the most important connections used in structures. The stiffnesses of the bolt and of the connected members are the primary qualities that control the lifetime of the connection. The stiffness of the bolt can be estimated rather easily, in contrast to the member stiffness......, but with finite element (FE) and contact analysis, it is possible to find the stiffness of the member. In the case of many connections and for practical applications, it is not suitable to make a full FE analysis. The purpose of the present paper is to find simplified expressions for the stiffness of the member......, including the case when the width of the member is limited. The calculation of the stiffness is based on the FE, including the solution to the contact problem, and we express the stiffness as a function of the elastic energy in the structure, whereby the definition of the displacements related...

  16. Experimental and Analytical Analysis of Perforated Plate Aerodynamics

    Institute of Scientific and Technical Information of China (English)

    Jürgen Zierep; Rainer Bohning; Piotr Doerffer

    2003-01-01

    Perforated walls and transpiration flow play an important role in aerodynamics due to an increasing interest in application of flow control by means of blowing and/or suction. An experimental study was carried out which has led to the determination of a transpiration flow characteristics in the form of a simple formula that is very useful in modelling such flows. In connection to this relation a method of "aerodynamic porosity" determination has been proposed which is much more reliable than geometric description of the porosity. A theoretical analysis of the flow through a perforation hole was also carried out. The flow was considered as compressible and viscous. The gasdynamic analysis led us to a very similar result to the relation obtained from the experiment. The adequacy of the theoretical result is discussed in respect to the experiment.

  17. Analysis of overdispersed count data by mixtures of Poisson variables and Poisson processes.

    Science.gov (United States)

    Hougaard, P; Lee, M L; Whitmore, G A

    1997-12-01

    Count data often show overdispersion compared to the Poisson distribution. Overdispersion is typically modeled by a random effect for the mean, based on the gamma distribution, leading to the negative binomial distribution for the count. This paper considers a larger family of mixture distributions, including the inverse Gaussian mixture distribution. It is demonstrated that it gives a significantly better fit for a data set on the frequency of epileptic seizures. The same approach can be used to generate counting processes from Poisson processes, where the rate or the time is random. A random rate corresponds to variation between patients, whereas a random time corresponds to variation within patients.

  18. Network meta-analysis of (individual patient) time to event data alongside (aggregate) count data.

    Science.gov (United States)

    Saramago, Pedro; Chuang, Ling-Hsiang; Soares, Marta O

    2014-09-10

    Network meta-analysis methods extend the standard pair-wise framework to allow simultaneous comparison of multiple interventions in a single statistical model. Despite published work on network meta-analysis mainly focussing on the synthesis of aggregate data, methods have been developed that allow the use of individual patient-level data specifically when outcomes are dichotomous or continuous. This paper focuses on the synthesis of individual patient-level and summary time to event data, motivated by a real data example looking at the effectiveness of high compression treatments on the healing of venous leg ulcers. This paper introduces a novel network meta-analysis modelling approach that allows individual patient-level (time to event with censoring) and summary-level data (event count for a given follow-up time) to be synthesised jointly by assuming an underlying, common, distribution of time to healing. Alternative model assumptions were tested within the motivating example. Model fit and adequacy measures were used to compare and select models. Due to the availability of individual patient-level data in our example we were able to use a Weibull distribution to describe time to healing; otherwise, we would have been limited to specifying a uniparametric distribution. Absolute effectiveness estimates were more sensitive than relative effectiveness estimates to a range of alternative specifications for the model. The synthesis of time to event data considering individual patient-level data provides modelling flexibility, and can be particularly important when absolute effectiveness estimates, and not just relative effect estimates, are of interest.

  19. Characterization and analysis of surface notches on Ti-alloy plates fabricated by additive manufacturing techniques

    Science.gov (United States)

    Chan, Kwai S.

    2015-12-01

    Rectangular plates of Ti-6Al-4V with extra low interstitial (ELI) were fabricated by layer-by-layer deposition techniques that included electron beam melting (EBM) and laser beam melting (LBM). The surface conditions of these plates were characterized using x-ray micro-computed tomography. The depth and radius of surface notch-like features on the LBM and EBM plates were measured from sectional images of individual virtual slices of the rectangular plates. The stress concentration factors of individual surface notches were computed and analyzed statistically to determine the appropriate distributions for the notch depth, notch radius, and stress concentration factor. These results were correlated with the fatigue life of the Ti-6Al-4V ELI alloys from an earlier investigation. A surface notch analysis was performed to assess the debit in the fatigue strength due to the surface notches. The assessment revealed that the fatigue lives of the additively manufactured plates with rough surface topographies and notch-like features are dominated by the fatigue crack growth of large cracks for both the LBM and EBM materials. The fatigue strength reduction due to the surface notches can be as large as 60%-75%. It is concluded that for better fatigue performance, the surface notches on EBM and LBM materials need to be removed by machining and the surface roughness be improved to a surface finish of about 1 μm.

  20. 3-D vibration analysis of annular sector plates using the Chebyshev-Ritz method

    Science.gov (United States)

    Zhou, D.; Lo, S. H.; Cheung, Y. K.

    2009-02-01

    The three-dimensional free vibration of annular sector plates with various boundary conditions is studied by means of the Chebyshev-Ritz method. The analysis is based on the three-dimensional small strain linear elasticity theory. The product of Chebyshev polynomials satisfying the necessary boundary conditions is selected as admissible functions in such a way that the governing eigenvalue equation can be conveniently derived through an optimization process by the Ritz method. The boundary functions guarantee the satisfaction of the geometric boundary conditions of the plates and the Chebyshev polynomials provide the robustness for numerical calculation. The present study provides a full vibration spectrum for the thick annular sector plates, which cannot be given by the two-dimensional (2-D) theories such as the Mindlin theory. Comprehensive numerical results with high accuracy are systematically produced, which can be used as benchmark to evaluate other numerical methods. The effect of radius ratio, thickness ratio and sector angle on natural frequencies of the plates with a sector angle from 120° to 360° is discussed in detail. The three-dimensional vibration solutions for plates with a re-entrant sector angle (larger than 180°) and shallow helicoidal shells (sector angle larger than 360°) with a small helix angle are presented for the first time.

  1. FEM Analysis of Spring-backs in Age Forming of Aluminum Alloy Plates

    Institute of Scientific and Technical Information of China (English)

    Huang Lin; Wan Min; Chi Cailou; Ji Xiusheng

    2007-01-01

    The age forming technology, characterized by huge spring-backs, has been developed to manufacture large integral wing-skin panel parts, which necessitates devising a method of predicting spring-backs. A 7B04-T7451 aluminum alloy creep test in tension is accomplished at 155 ℃, and the creep curves are obtained. The material constants of the mechanism-based creep constitutive equations are determined through experiments. The age forming process and the spring-backs of 7B04 aluminum alloy plates are analyzed using the commercial finite element software ABAQUS. The effects of plate thickness and formingtime on spring-backs are researched. The spring-backs decrease with the increase of plate thickness and forming time. The test results verify the reliability of the finite element method (FEM) analysis.

  2. Theoretical analysis on shear-bending deflection of a ring-shape piezoelectric plate

    Directory of Open Access Journals (Sweden)

    Zejun Yu

    2016-02-01

    Full Text Available In this paper, the electromechanical coupling field in shear-bending mode for a ring-shape piezoelectric plate was theoretically established. According to the classical small bending elastic plate theory and piezoelectric constitutive equations, the analytical solution to the bending deformation of the piezo-actuator under electric field and a concentrated or uniformly distributed mechanical load was achieved. The mechanism for generating bending deformation is attributed to axisymmetric shear strain, which further induces the bending deformation of the single ring-shape piezoelectric plate. This mechanism is significant different from that of piezoelectric bimorph or unimorph actuators reported before. Our analysis offers guidance for the optimum design of a ring-shape shear-bending piezo-actuator.

  3. Dynamic Analysis of the Titanium Alloy Plate under Thermal-acoustic Loadings

    Directory of Open Access Journals (Sweden)

    Zou Xuefeng

    2015-01-01

    Full Text Available Hypersonic vehicles structures suffer complex combined loadings generally. For the thin-walled structures and thermal protection systems of the aircraft, high temperature and intensity acoustic loadings are the significant factors that leading to their break. The object of this paper is typical simply supported titanium alloy plate, the finite element method was adopted to calculate the critical thermal buckling temperature the ordinal coupling method and Newmark method were adopted to calculate the thermal-acoustic dynamic response. Based on the FEM analysis, the power spectrum densities (PSD of center point was presented. Research results show that the thermal buckling of the typical simply supported titanium alloy plate occurs easily because of the low critical thermal buckling temperature, dynamic response of the thermal buckled plate suffering acoustic loads performs strong nonlinear characteristics and complex forms of exercise.

  4. A refined mixed shear flexible finite element for the nonlinear analysis of laminated plates

    Science.gov (United States)

    Putcha, N. S.; Reddy, J. N.

    1986-01-01

    The present study is concerned with the development of a mixed shear flexible finite element with relaxed continuity for the geometrically linear and nonlinear analysis of laminated anisotropic plates. The formulation of the element is based on a refined higher-order theory. This theory satisfies the zero transverse shear stress boundary conditions on the top and bottom faces of the plate. Shear correction coefficients are not needed. The developed element consists of 11 degrees-of-freedom per node, taking into account three displacements, two rotations, and six moment resultants. An evaluation of the element is conducted with respect to the accuracy obtained in the bending of laminated anistropic rectangular plates with different lamination schemes, loadings, and boundary conditions.

  5. Nonlinear Forced Vibration Analysis for Thin Rectangular Plate on Nonlinear Elastic Foundation

    Directory of Open Access Journals (Sweden)

    Zhong Zhengqiang

    2013-02-01

    Full Text Available Nonlinear forced vibration is analyzed for thin rectangular plate with four free edges on nonlinear elastic foundation. Based on Hamilton variation principle, equations of nonlinear vibration motion for thin rectangular plate under harmonic loads on nonlinear elastic foundation are established. In the case of four free edges, viable expressions of trial functions for this specification are proposed, satisfying all boundary conditions. Then, equations are transformed to a system of nonlinear algebraic equations by using Galerkin method and are solved by using harmonic balance method. In the analysis of numerical computations, the effect on the amplitude-frequency characteristic curve due to change of the structural parameters of plate, parameters of foundation and parameters of excitation force are discussed.

  6. Titanium dioxide coated MALDI plate for on target analysis of phosphopeptides.

    Science.gov (United States)

    Torta, Federico; Fusi, Matteo; Casari, Carlo S; Bottani, Carlo E; Bachi, Angela

    2009-04-01

    Protein phosphorylation controls many cellular processes and activities. One of the major challenges in the proteomic study of phosphorylation is the enrichment of substoichiometric phosphorylated peptides from complex mixtures. Titanium dioxide (TiO2)-based chromatography is now widely applied to isolate phosphopeptides because of its efficiency and flexibility. In this study, a novel TiO2 coated matrix assisted laser desorption ionization plate is presented and tested for the purification of phosphopeptides from complex mixtures. The novel feature of this approach is the deposition of a nanostructured TiO2 film on stainless steel plates by pulsed laser deposition (PLD). By using tryptic digests of alpha-casein, beta-casein, and other nonphosphorylated proteins, the successful enrichment of phosphopeptides was possible with this novel device, called T-plate, even when working in the low fmol range, making the sample ready for mass spectrometric analysis in few minutes.

  7. The VLA-COSMOS Survey: III. Further Catalog Analysis and the Radio Source Counts

    CERN Document Server

    Bondi, M; Schinnerer, E; Smolcic, V; Jahnke, K; Carilli, C; Zamorani, G

    2008-01-01

    The VLA-COSMOS Large Project has imaged the 2 sq.deg. COSMOS field with a resolution of 1.5 arcsec and a sensitivity of about 11 microJy (1 sigma) yielding to a catalog of about 3600 radio sources. In this paper we present a further analysis of the VLA-COSMOS Large Project catalog of radio sources aimed to: 1) quantify and correct for the effect of bandwidth smearing in the catalog, 2) determine the incompleteness produced by the noise bias and the resolution bias in the new catalog and 3) derive the radio source counts at 1.4 GHz. The effect of bandwidth smearing on the radio sources in the catalog was quantified comparing the peak and total flux densities in the final mosaic and in each of the individual pointings where the source was closest to the center of the field. We find that the peak flux densities in the original VLA-COSMOS Large Project catalog have to be divided by a factor about 0.8 or 0.9, depending on the distance from the mosaic center. The completeness of the radio catalog has been tested us...

  8. Food insecurity and low CD4 count among HIV-infected people: a systematic review and meta-analysis.

    Science.gov (United States)

    Aibibula, Wusiman; Cox, Joseph; Hamelin, Anne-Marie; Mamiya, Hiroshi; Klein, Marina B; Brassard, Paul

    2016-12-01

    Food insecurity is defined as a limited or uncertain ability to acquire acceptable foods in socially acceptable ways, or limited or uncertain availability of nutritionally adequate and safe foods. While effective antiretroviral treatment can significantly increase CD4 counts in the majority of patients, there are certain populations who remain at relatively low CD4 count levels. Factors possibly associated with poor CD4 recovery have been extensively studied, but the association between food insecurity and low CD4 count is inconsistent in the literature. The objective is to systematically review published literature to determine the association between food insecurity and CD4 count among HIV-infected people. PubMed, Web of Science, ProQuest ABI/INFORM Complete, Ovid Medline and EMBASE Classic, plus bibliographies of relevant studies were systematically searched up to May 2015, where the earliest database coverage started from 1900. Studies that quantitatively assessed the association between food insecurity and CD4 count among HIV-infected people were eligible for inclusion. Study results were summarized using random effects model. A total of 2093 articles were identified through electronic database search and manual bibliographic search, of which 8 studies included in this meta-analysis. Food insecure people had 1.32 times greater odds of having lower CD4 counts compared to food secure people (OR = 1.32, 95% CI: 1.15-1.53) and food insecure people had on average 91 fewer CD4 cells/µl compared to their food secure counterparts (mean difference = -91.09, 95% CI: -156.16, -26.02). Food insecurity could be a potential barrier to immune recovery as measured by CD4 counts among HIV-infected people.

  9. Specific on-plate enrichment of phosphorylated peptides for direct MALDI-TOF MS analysis.

    Science.gov (United States)

    Qiao, Liang; Roussel, Christophe; Wan, Jingjing; Yang, Pengyuan; Girault, Hubert H; Liu, Baohong

    2007-12-01

    An on-plate specific enrichment method is presented for the direct analysis of peptides phosphorylation. An array of sintered TiO 2 nanoparticle spots was prepared on a stainless steel plate to provide porous substrate with a very large specific surface and durable functions. These spots were used to selectively capture phosphorylated peptides from peptide mixtures, and the immobilized phosphopeptides could then be analyzed directly by MALDI MS after washing away the nonphosphorylated peptides. beta-Casein and protein mixtures were employed as model samples to investigate the selection efficiency. In this strategy, the steps of phosphopeptide capture, purification, and subsequent mass spectrometry analysis are all successfully accomplished on a single target plate, which greatly reduces sample loss and simplifies analytical procedures. The low detection limit, small sample size, and rapid selective entrapment show that this on-plate strategy is promising for online enrichment of phosphopeptides, which is essential for the analysis of minute amount of samples in high-throughput proteome research.

  10. Analysis of Nonlinear Vibration of Hard Coating Thin Plate by Finite Element Iteration Method

    Directory of Open Access Journals (Sweden)

    Hui Li

    2014-01-01

    Full Text Available This paper studies nonlinear vibration mechanism of hard coating thin plate based on macroscopic vibration theory and proposes finite element iteration method (FEIM to theoretically calculate its nature frequency and vibration response. First of all, strain dependent mechanical property of hard coating is briefly introduced and polynomial method is adopted to characterize the storage and loss modulus of coating material. Then, the principle formulas of inherent and dynamic response characteristics of the hard coating composite plate are derived. And consequently specific analysis procedure is proposed by combining ANSYS APDL and self-designed MATLAB program. Finally, a composite plate coated with MgO + Al2O3 is taken as a study object and both nonlinear vibration test and analysis are conducted on the plate specimen with considering strain dependent mechanical parameters of hard coating. Through comparing the resulting frequency and response results, the practicability and reliability of FEIM have been verified and the corresponding analysis results can provide an important reference for further study on nonlinear vibration mechanism of hard coating composite structure.

  11. Static & Thermal Analysis of Positive Multiple Friction Plate using FEA Package

    Directory of Open Access Journals (Sweden)

    Shaik Mohammad Ali

    2014-11-01

    Full Text Available Clutch is a mechanical device, which is used to engage or disengage the source of power from the rest of the power transmission system at the operator’s will. The clutch can connect or disconnect the driving shaft from the driven shaft when necessary. Clutches are designed to transfer maximum torque with minimum heat generation. During engagement and disengagement the two clutch discs has the sliding motion between them. The project contains that designing and analysis of two positive multi friction plates. For the designing of the friction plates 3d modeling software used and for the analysis ansys package is used. In the analysis part the two models are analyzed with different materials by conducting two types of analysis which are structural and thermal. Structural analysis is done to find out the stress values and the thermal analysis done to find out the temperature distribution on the model. By these two analysis results we are suggesting the best material to the effective model of the multiple friction plate.

  12. Blast counts in bone marrow aspirate smears: analysis using the poisson probability function, bayes theorem, and information theory.

    Science.gov (United States)

    Vollmer, Robin T

    2009-02-01

    Counts of cells or other phenomena observed through a microscope are numeric observations and, as such, are subject to mathematical and statistical analyses. For example, the Poisson probability function provides the probability of observing a particular number of blasts in a bone marrow aspirate, given an underlying true fraction of blasts present and a particular number of cells evaluated. Furthermore, using the Poisson function, Bayes theorem can provide the probabilities of specific categories of refractory anemia, given a number of observed blasts in a specific total of cells evaluated. Herein, I introduce and demonstrate these mathematical functions for the analysis of counts of blasts in marrow aspirates and explore the uncertainty that naturally arises when counts of blasts are near cut points used to separate the categories of refractory anemia without excess blasts, refractory anemia with excess blasts, and acute leukemia.

  13. Automatic Analysis of Cellularity in Glioblastoma and Correlation with ADC Using Trajectory Analysis and Automatic Nuclei Counting

    Science.gov (United States)

    Burth, Sina; Kieslich, Pascal J.; Jungk, Christine; Sahm, Felix; Kickingereder, Philipp; Kiening, Karl; Unterberg, Andreas; Wick, Wolfgang; Schlemmer, Heinz-Peter; Bendszus, Martin; Radbruch, Alexander

    2016-01-01

    Objective Several studies have analyzed a correlation between the apparent diffusion coefficient (ADC) derived from diffusion-weighted MRI and the tumor cellularity of corresponding histopathological specimens in brain tumors with inconclusive findings. Here, we compared a large dataset of ADC and cellularity values of stereotactic biopsies of glioblastoma patients using a new postprocessing approach including trajectory analysis and automatic nuclei counting. Materials and Methods Thirty-seven patients with newly diagnosed glioblastomas were enrolled in this study. ADC maps were acquired preoperatively at 3T and coregistered to the intraoperative MRI that contained the coordinates of the biopsy trajectory. 561 biopsy specimens were obtained; corresponding cellularity was calculated by semi-automatic nuclei counting and correlated to the respective preoperative ADC values along the stereotactic biopsy trajectory which included areas of T1-contrast-enhancement and necrosis. Results There was a weak to moderate inverse correlation between ADC and cellularity in glioblastomas that varied depending on the approach towards statistical analysis: for mean values per patient, Spearman’s ρ = -0.48 (p = 0.002), for all trajectory values in one joint analysis Spearman’s ρ = -0.32 (p < 0.001). The inverse correlation was additionally verified by a linear mixed model. Conclusions Our data confirms a previously reported inverse correlation between ADC and tumor cellularity. However, the correlation in the current article is weaker than the pooled correlation of comparable previous studies. Hence, besides cell density, other factors, such as necrosis and edema might influence ADC values in glioblastomas. PMID:27467557

  14. Investigation of Shear Stud Performance in Flat Plate Using Finite Element Analysis

    Directory of Open Access Journals (Sweden)

    T.S. Viswanathan

    2014-09-01

    Full Text Available Three types of shear stud arrangement, respectively featuring an orthogonal, a radial and a critical perimeter pattern, were evaluated numerically. A numerical investigation was conducted using the finite element software ABAQUS to evaluate their ability to resist punching shear in a flat plate. The finite element analysis here is an application of the nonlinear analysis of reinforced concrete structures using three-dimensional solid finite elements. The nonlinear characteristics of concrete were achieved by employing the concrete damaged plasticity model in the finite element program. Transverse shear stress was evaluated using finite element analysis in terms of shear stress distribution for flat plate with and without shear stud reinforcement. The model predicted that shear studs placed along the critical perimeter are more effective compared to orthogonal and radial patterns.

  15. Three-dimensional structural analysis of the plate-fin heat exchanger

    Energy Technology Data Exchange (ETDEWEB)

    Nakagawa, T.; Sou, T.

    1984-06-01

    The Brazed aluminum plate-fin heat exchanger is a complex structure consisting of a core, headers and nozzles. The core is built of many layers of flat parting sheets and corrugated fins, and is sealed by side bars. Stress patterns in this type of heat exchanger have so far not been accurately analyzed, due to the complexity of the structure. A three dimensional structural analysis of such a core-header-nozzle structure subject to internal pressure is performed herein, using the finite element method, in order to investigate the mechanical characteristics of the structure. In the analysis, the corrugated fin is modeled by an equivalent anisotropic continuum element, to save on the computational cost. The adequacy of the analysis is then verified by performing a strain measurement test on the actual plate-fin heat exchanger. On the basis of the analytical results, it becomes clear that some critical parts need special attention when designing such structures.

  16. Bias correction and Bayesian analysis of aggregate counts in SAGE libraries

    Directory of Open Access Journals (Sweden)

    Briggs William M

    2010-02-01

    Full Text Available Abstract Background Tag-based techniques, such as SAGE, are commonly used to sample the mRNA pool of an organism's transcriptome. Incomplete digestion during the tag formation process may allow for multiple tags to be generated from a given mRNA transcript. The probability of forming a tag varies with its relative location. As a result, the observed tag counts represent a biased sample of the actual transcript pool. In SAGE this bias can be avoided by ignoring all but the 3' most tag but will discard a large fraction of the observed data. Taking this bias into account should allow more of the available data to be used leading to increased statistical power. Results Three new hierarchical models, which directly embed a model for the variation in tag formation probability, are proposed and their associated Bayesian inference algorithms are developed. These models may be applied to libraries at both the tag and aggregate level. Simulation experiments and analysis of real data are used to contrast the accuracy of the various methods. The consequences of tag formation bias are discussed in the context of testing differential expression. A description is given as to how these algorithms can be applied in that context. Conclusions Several Bayesian inference algorithms that account for tag formation effects are compared with the DPB algorithm providing clear evidence of superior performance. The accuracy of inferences when using a particular non-informative prior is found to depend on the expression level of a given gene. The multivariate nature of the approach easily allows both univariate and joint tests of differential expression. Calculations demonstrate the potential for false positive and negative findings due to variation in tag formation probabilities across samples when testing for differential expression.

  17. Anarthria impairs subvocal counting.

    Science.gov (United States)

    Cubelli, R; Nichelli, P; Pentore, R

    1993-12-01

    We studied subvocal counting in two pure anarthric patients. Analysis showed that they performed definitively worse than normal subjects free to articulate subvocally and their scores were in the lower bounds of the performances of subjects suppressing articulation. These results suggest that subvocal counting is impaired after anarthria.

  18. Approaches for modeling within subject variability in pharmacometric count data analysis: dynamic inter-occasion variability and stochastic differential equations.

    Science.gov (United States)

    Deng, Chenhui; Plan, Elodie L; Karlsson, Mats O

    2016-06-01

    Parameter variation in pharmacometric analysis studies can be characterized as within subject parameter variability (WSV) in pharmacometric models. WSV has previously been successfully modeled using inter-occasion variability (IOV), but also stochastic differential equations (SDEs). In this study, two approaches, dynamic inter-occasion variability (dIOV) and adapted stochastic differential equations, were proposed to investigate WSV in pharmacometric count data analysis. These approaches were applied to published count models for seizure counts and Likert pain scores. Both approaches improved the model fits significantly. In addition, stochastic simulation and estimation were used to explore further the capability of the two approaches to diagnose and improve models where existing WSV is not recognized. The results of simulations confirmed the gain in introducing WSV as dIOV and SDEs when parameters vary randomly over time. Further, the approaches were also informative as diagnostics of model misspecification, when parameters changed systematically over time but this was not recognized in the structural model. The proposed approaches in this study offer strategies to characterize WSV and are not restricted to count data.

  19. Counting radon tracks in Makrofol detectors with the 'image reduction and analysis facility' (IRAF) software package

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez, F. [Laboratorio de Fisica Medica y Radioactividad Ambiental, Departamento de Medicina Fisica y Farmacologia, Universidad de La Laguna, 38320 La Laguna, Tenerife (Spain)]. E-mail: fimerall@ull.es; Gonzalez-Manrique, S. [Laboratorio de Fisica Medica y Radioactividad Ambiental, Departamento de Medicina Fisica y Farmacologia, Universidad de La Laguna, 38320 La Laguna, Tenerife (Spain); Karlsson, L. [Laboratorio de Fisica Medica y Radioactividad Ambiental, Departamento de Medicina Fisica y Farmacologia, Universidad de La Laguna, 38320 La Laguna, Tenerife (Spain); Hernandez-Armas, J. [Laboratorio de Fisica Medica y Radioactividad Ambiental, Departamento de Medicina Fisica y Farmacologia, Universidad de La Laguna, 38320 La Laguna, Tenerife (Spain); Aparicio, A. [Instituto de Astrofisica de Canarias, 38200 La Laguna, Tenerife (Spain); Departamento de Astrofisica, Universidad de La Laguna. Avenida. Astrofisico Francisco Sanchez s/n, 38071 La Laguna, Tenerife (Spain)

    2007-03-15

    Makrofol detectors are commonly used for long-term radon ({sup 222}Rn) measurements in houses, schools and workplaces. The use of this type of passive detectors for the determination of radon concentrations requires the counting of the nuclear tracks produced by alpha particles on the detecting material. The 'image reduction and analysis facility' (IRAF) software package is a piece of software commonly used in astronomical applications. It allows detailed counting and mapping of sky sections where stars are grouped very closely, even forming clusters. In order to count the nuclear tracks in our Makrofol radon detectors, we have developed an inter-disciplinary application that takes advantage of the similitude that exist between counting stars in a dark sky and tracks in a track-etch detector. Thus, a low cost semi-automatic system has been set up in our laboratory which utilises a commercially available desktop scanner and the IRAF software package. A detailed description of the proposed semi-automatic method and its performance, in comparison to ocular counting, is described in detail here. In addition, the calibration factor for this procedure, 2.97+/-0.07kBqm{sup -3}htrack{sup -1}cm{sup 2}, has been calculated based on the results obtained from exposing 46 detectors to certified radon concentrations. Furthermore, the results of a preliminary radon survey carried out in 62 schools in Tenerife island (Spain), using Makrofol detectors, counted with the mentioned procedure, are briefly presented. The results reported here indicate that the developed procedure permits a fast, accurate and unbiased determination of the radon tracks in a large number of detectors. The measurements carried out in the schools showed that the radon concentrations in at least 12 schools were above 200Bqm{sup -3} and, in two of them, above 400Bqm{sup -3}. Further studies should be performed at those schools following the European Union recommendations about radon concentrations in

  20. Genetic modifiers of neurofibromatosis type 1-associated cafe-au-lait macule count identified using multi-platform analysis.

    Directory of Open Access Journals (Sweden)

    Alexander Pemov

    2014-10-01

    Full Text Available Neurofibromatosis type 1 (NF1 is an autosomal dominant, monogenic disorder of dysregulated neurocutaneous tissue growth. Pleiotropy, variable expressivity and few NF1 genotype-phenotype correlates limit clinical prognostication in NF1. Phenotype complexity in NF1 is hypothesized to derive in part from genetic modifiers unlinked to the NF1 locus. In this study, we hypothesized that normal variation in germline gene expression confers risk for certain phenotypes in NF1. In a set of 79 individuals with NF1, we examined the association between gene expression in lymphoblastoid cell lines with NF1-associated phenotypes and sequenced select genes with significant phenotype/expression correlations. In a discovery cohort of 89 self-reported European-Americans with NF1 we examined the association between germline sequence variants of these genes with café-au-lait macule (CALM count, a tractable, tumor-like phenotype in NF1. Two correlated, common SNPs (rs4660761 and rs7161 between DPH2 and ATP6V0B were significantly associated with the CALM count. Analysis with tiled regression also identified SNP rs4660761 as significantly associated with CALM count. SNP rs1800934 and 12 rare variants in the mismatch repair gene MSH6 were also associated with CALM count. Both SNPs rs7161 and rs4660761 (DPH2 and ATP6V0B were highly significant in a mega-analysis in a combined cohort of 180 self-reported European-Americans; SNP rs1800934 (MSH6 was near-significant in a meta-analysis assuming dominant effect of the minor allele. SNP rs4660761 is predicted to regulate ATP6V0B, a gene associated with melanosome biology. Individuals with homozygous mutations in MSH6 can develop an NF1-like phenotype, including multiple CALMs. Through a multi-platform approach, we identified variants that influence NF1 CALM count.

  1. Genetic modifiers of neurofibromatosis type 1-associated café-au-lait macule count identified using multi-platform analysis.

    Directory of Open Access Journals (Sweden)

    Alexander Pemov

    2014-10-01

    Full Text Available Neurofibromatosis type 1 (NF1 is an autosomal dominant, monogenic disorder of dysregulated neurocutaneous tissue growth. Pleiotropy, variable expressivity and few NF1 genotype-phenotype correlates limit clinical prognostication in NF1. Phenotype complexity in NF1 is hypothesized to derive in part from genetic modifiers unlinked to the NF1 locus. In this study, we hypothesized that normal variation in germline gene expression confers risk for certain phenotypes in NF1. In a set of 79 individuals with NF1, we examined the association between gene expression in lymphoblastoid cell lines with NF1-associated phenotypes and sequenced select genes with significant phenotype/expression correlations. In a discovery cohort of 89 self-reported European-Americans with NF1 we examined the association between germline sequence variants of these genes with café-au-lait macule (CALM count, a tractable, tumor-like phenotype in NF1. Two correlated, common SNPs (rs4660761 and rs7161 between DPH2 and ATP6V0B were significantly associated with the CALM count. Analysis with tiled regression also identified SNP rs4660761 as significantly associated with CALM count. SNP rs1800934 and 12 rare variants in the mismatch repair gene MSH6 were also associated with CALM count. Both SNPs rs7161 and rs4660761 (DPH2 and ATP6V0B were highly significant in a mega-analysis in a combined cohort of 180 self-reported European-Americans; SNP rs1800934 (MSH6 was near-significant in a meta-analysis assuming dominant effect of the minor allele. SNP rs4660761 is predicted to regulate ATP6V0B, a gene associated with melanosome biology. Individuals with homozygous mutations in MSH6 can develop an NF1-like phenotype, including multiple CALMs. Through a multi-platform approach, we identified variants that influence NF1 CALM count.

  2. Single Photon Counting Performance and Noise Analysis of CMOS SPAD-Based Image Sensors.

    Science.gov (United States)

    Dutton, Neale A W; Gyongy, Istvan; Parmesan, Luca; Henderson, Robert K

    2016-07-20

    SPAD-based solid state CMOS image sensors utilising analogue integrators have attained deep sub-electron read noise (DSERN) permitting single photon counting (SPC) imaging. A new method is proposed to determine the read noise in DSERN image sensors by evaluating the peak separation and width (PSW) of single photon peaks in a photon counting histogram (PCH). The technique is used to identify and analyse cumulative noise in analogue integrating SPC SPAD-based pixels. The DSERN of our SPAD image sensor is exploited to confirm recent multi-photon threshold quanta image sensor (QIS) theory. Finally, various single and multiple photon spatio-temporal oversampling techniques are reviewed.

  3. Nickel Electroless Plating: Adhesion Analysis for Mono-Type Crystalline Silicon Solar Cells.

    Science.gov (United States)

    Shin, Eun Gu; Rehman, Atteq ur; Lee, Sang Hee; Lee, Soo Hong

    2015-10-01

    The adhesion of the front electrodes to silicon substrate is the most important parameters to be optimized. Nickel silicide which is formed by sintering process using a silicon substrate improves the mechanical and electrical properties as well as act as diffusion barrier for copper. In this experiment p-type mono-crystalline czochralski (CZ) silicon wafers having resistivity of 1.5 Ω·cm were used to study one step and two step nickel electroless plating process. POCl3 diffusion process was performed to form the emitter with the sheet resistance of 70 ohm/sq. The Six, layer was set down as an antireflection coating (ARC) layer at emitter surface by plasma enhanced chemical vapor deposition (PECVD) process. Laser ablation process was used to open SiNx passivation layer locally for the formation of the front electrodes. Nickel was deposited by electroless plating process by one step and two step nickel electroless deposition process. The two step nickel plating was performed by applying a second nickel deposition step subsequent to the first sintering process. Furthermore, the adhesion analysis for both one step and two steps process was conducted using peel force tester (universal testing machine, H5KT) after depositing Cu contact by light induced plating (LIP).

  4. Buckling Analysis of Functionally Graded Material Plates Using Higher Order Shear Deformation Theory

    Directory of Open Access Journals (Sweden)

    B. Sidda Reddy

    2013-01-01

    Full Text Available The prime aim of the present study is to present analytical formulations and solutions for the buckling analysis of simply supported functionally graded plates (FGPs using higher order shear deformation theory (HSDT without enforcing zero transverse shear stresses on the top and bottom surfaces of the plate. It does not require shear correction factors and transverse shear stresses vary parabolically across the thickness. Material properties of the plate are assumed to vary in the thickness direction according to a power law distribution in terms of the volume fractions of the constituents. The equations of motion and boundary conditions are derived using the principle of virtual work. Solutions are obtained for FGPs in closed-form using Navier’s technique. Comparison studies are performed to verify the validity of the present results from which it can be concluded that the proposed theory is accurate and efficient in predicting the buckling behavior of functionally graded plates. The effect of side-to-thickness ratio, aspect ratio, modulus ratio, the volume fraction exponent, and the loading conditions on the critical buckling load of FGPs is also investigated and discussed.

  5. Elasto-plastic buckling analysis of laminated plates including interfacial damage

    Energy Technology Data Exchange (ETDEWEB)

    Tian, Yanping; Fu, Yiming [Hunan University, State Key Laboratory of Advanced Technology of Design and Manufacturing for Vehicle Body, College of Mechanics and Aerospace, Changsha (China)

    2010-06-15

    Elasto-plastic buckling of orthotropic laminated plates, which include interfacial damage, is analyzed in detail. Firstly, a novel mixed hardening yield criterion, as an improvement of Hill's counterpart, is proposed for the orthotropic materials on the basis of the plastic theory. And differing from Hill's theory, the present yield criterion is related to the spherical tensor of stress. Then, the incremental elasto-plastic constitutive relations of the mixed hardening orthotropic materials are presented. Secondly, the incremental static equilibrium equations for laminated plates including interfacial damage are established based on Von-Karman type theory and the principle of minimum potential energy. Finally, the elasto-plastic buckling of laminated plates are solved by adopting the Galerkin method and iteration scheme. The numerical results show that buckling of the plate occurs easier due to the existence of interfacial damage, and the critical load trends to constant when the interfacial damage approaches a certain degree. Also, the effect of anisotropy on buckling is obvious and the analysis of elasto-plastic buckling is necessary. (orig.)

  6. Vibration analysis of cracked plates using the extended finite element method

    Energy Technology Data Exchange (ETDEWEB)

    Bachene, M. [University of Medea, Department of Mechanical Engineering, Medea (Algeria); Tiberkak, R. [University of Blida, Department of Mechanical Engineering, Blida (Algeria); Rechak, S. [National Polytechnic School of Algiers, Laboratory of Mechanical Engineering and Development, Algiers (Algeria)

    2009-03-15

    In the present paper, the extended finite element method (X-FEM) is adopted to analyze vibrations of cracked plates. Mindlin's plate theory taking into account the effects of shear deformation and rotatory inertia is included in the development of the model. First, conventional FEM without any discontinuity is carried out, then the enrichment proposed by Moes et al. (Int J Numer Methods Eng 46, 131-150, 1999) of nodal elements containing cracks is added to the FEM formulation. Numerical implementation of enriched elements by discontinuous functions is performed, and thus dynamic equations (stiffness and mass matrices) are established. A FORTRAN computer code based on the X-FEM formulation is hence developed. Rectangular and square plates containing through-edge and central cracks with different boundary conditions are considered. The subspace iteration method is used to solve the eigenvalue problem. Natural frequencies as well as the corresponding eigenfunctions are consequently calculated as a function of the crack length. The obtained results show that the X-FEM is an efficient method in the dynamic analysis of plates containing discontinuities. (orig.)

  7. Second law analysis of a plate heat exchanger with an axial dispersive wave

    Science.gov (United States)

    Kumar Das, Sarit; Roetzel, Wilfried

    A second law analysis is presented for thermally dispersive flow through a plate heat exchanger. It is well known that in plate or plate fin type heat exchangers the backmixing and other deviations from plug flow contribute significantly to the inefficiency of the heat exchanger, which is of importance to heat exchangers working in the cryogenic regime. The conventional axial heat dispersion model which is used so far is found to be better than `plug flow' model but still unsatisfactory where the timescale related to heat transfer is comparable with the thermal relaxation time for the propagation of dispersion. The present work therefore considers dispersion as a wave phenomenon propagating with a finite velocity. The study discusses the nature of variation of different contributions to total exergy loss in the heat exchanger with respect to dispersion parameters of the Peclet number and propagation velocity of the dispersive wave. The practical example of the single-pass plate heat exchanger demonstrates how a second law optimization can be carried out for heat transfer equipment under such conditions.

  8. Multiplicity Counting

    Energy Technology Data Exchange (ETDEWEB)

    Geist, William H. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-12-01

    This set of slides begins by giving background and a review of neutron counting; three attributes of a verification item are discussed: 240Pueff mass; α, the ratio of (α,n) neutrons to spontaneous fission neutrons; and leakage multiplication. It then takes up neutron detector systems – theory & concepts (coincidence counting, moderation, die-away time); detector systems – some important details (deadtime, corrections); introduction to multiplicity counting; multiplicity electronics and example distributions; singles, doubles, and triples from measured multiplicity distributions; and the point model: multiplicity mathematics.

  9. Finite element analysis of the stability of combined plate internal fixation in posterior wall fractures of acetabulum.

    Science.gov (United States)

    Liu, Xi-Ming; Pan, Chang-Wu; Wang, Guo-Dong; Cai, Xian-Hua; Chen, Lei; Meng, Cheng-Fei; Huang, Jin-Cheng

    2015-01-01

    This study aims to explore the mechanical stability of combined plate internal fixation in posterior wall fractures of the acetabulum. The fracture and internal fixation models were established in this study and they were divided into four kinds of internal fixation models, finite element analysis was performed. The four groups were 2 mini-plates and 1 reconstruction plate fixation (A), Reconstruction plate internal fixation group (B), 2 screws internal fixation group (C) and mini-plates internal fixation group (D). The displacement of each node was measured and evaluated. There was no distortion in the geometric shape of the finite element model. The results of stress showed that it was less in the anterior pelvic ring and distributed uniform in labrum acetabulare; the stress was bigger in the upper and middle of sacroiliac joint and sciatic notch in sitting position. Combined plate internal fixation for posterior wall fractures of acetabular were stable and reliable, it is better than the other three methods.

  10. Flat plate vs. concentrator solar photovoltaic cells - A manufacturing cost analysis

    Science.gov (United States)

    Granon, L. A.; Coleman, M. G.

    1980-01-01

    The choice of which photovoltaic system (flat plate or concentrator) to use for utilizing solar cells to generate electricity depends mainly on the cost. A detailed, comparative manufacturing cost analysis of the two types of systems is presented. Several common assumptions, i.e., cell thickness, interest rate, power rate, factory production life, polysilicon cost, and direct labor rate are utilized in this analysis. Process sequences, cost variables, and sensitivity analyses have been studied, and results of the latter show that the most important parameters which determine manufacturing costs are concentration ratio, manufacturing volume, and cell efficiency. The total cost per watt of the flat plate solar cell is $1.45, and that of the concentrator solar cell is $1.85, the higher cost being due to the increased process complexity and material costs.

  11. Dimensional analysis of detrimental ozone generation by positive wire-to-plate corona discharge in air

    Science.gov (United States)

    Bo, Z.; Chen, J. H.

    2010-02-01

    The dimensional analysis technique is used to formulate a correlation between ozone generation rate and various parameters that are important in the design and operation of positive wire-to-plate corona discharges in indoor air. The dimensionless relation is determined by linear regression analysis based on the results from 36 laboratory-scale experiments. The derived equation is validated by experimental data and a numerical model published in the literature. Applications of such derived equation are illustrated through an example selection of the appropriate set of operating conditions in the design/operation of a photocopier to follow the federal regulations of ozone emission. Finally, a new current-voltage characteristic equation is proposed for positive wire-to-plate corona discharges based on the derived dimensionless equation.

  12. Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells.

    Science.gov (United States)

    Roellig, Daniela; Tan-Cabugao, Johanna; Esaian, Sevan; Bronner, Marianne E

    2017-03-29

    The 'neural plate border' of vertebrate embryos contains precursors of neural crest and placode cells, both defining vertebrate characteristics. How these lineages segregate from neural and epidermal fates has been a matter of debate. We address this by performing a fine-scale quantitative temporal analysis of transcription factor expression in the neural plate border of chick embryos. The results reveal significant overlap of transcription factors characteristic of multiple lineages in individual border cells from gastrula through neurula stages. Cell fate analysis using a Sox2 (neural) enhancer reveals that cells that are initially Sox2+ cells can contribute not only to neural tube but also to neural crest and epidermis. Moreover, modulating levels of Sox2 or Pax7 alters the apportionment of neural tube versus neural crest fates. Our results resolve a long-standing question and suggest that many individual border cells maintain ability to contribute to multiple ectodermal lineages until or beyond neural tube closure.

  13. Numerical analysis of grid plate melting after a severe accident in a Fast-Breeder Reactor (FBR)

    Indian Academy of Sciences (India)

    A Jasmin Sudha; K Velusamy

    2013-12-01

    Fast breeder reactors (FBRs) are provided with redundant and diverse plant protection systems with a very low failure probability (<10-6/reactor year), making core disruptive accident (CDA), a beyond design basis event (BDBE). Nevertheless, safety analysis is carried out even for such events with a view to mitigate their consequences by providing engineered safeguards like the in-vessel core catcher. During a CDA, a significant fraction of the hot molten fuel moves downwards and gets relocated to the lower plate of grid plate. The ability of this plate to resist or delay relocation of core melt further has been investigated by developing appropriate mathematical models and translating them into a computer code HEATRAN-1. The core melt is a time dependent volumetric heat source because of the radioactive decay of the fission products which it contains. The code solves the nonlinear heat conduction equation including phase change. The analysis reveals that if the bottom of grid plate is considered to be adiabatic, melt-through of grid plate (i.e., melting of the entire thickness of the plate) occurs between 800 s and 1000 s depending upon the initial conditions. Knowledge of this time estimate is essential for defining the initial thermal load on the core catcher plate. If heat transfer from the bottom of grid plate to the underlying sodium is taken into account, then melt-through does not take place, but the temperature of grid plate is high enough to cause creep failure.

  14. ANALYSIS OF RESIDUAL STRESS IN THE METAL MATRIX COMPOSITE PLATES WITH CIRCULAR HOLES

    Directory of Open Access Journals (Sweden)

    Muzaffer TOPCU

    1999-02-01

    Full Text Available In this study, elasto-plastic stress analysis have been made for metal matrix composite plates containing a central hole subjected to uniaxial tension under various uniformly distributed loads. In the solution, ısoparametric rectangular elements with nine nodes have been used. In the reinforcement angles 0 and 90 degrees for different diameters of the holes ın the vicinity of the holes residual stress variations have been investigated.

  15. Statistical analysis of the fluctuating counts of fecal bacteria in the water of Lake Kinneret.

    Science.gov (United States)

    Hadas, Ora; Corradini, Maria G; Peleg, Micha

    2004-01-01

    Counts of E. coli, Enteroccoci and fecal coliforms in four sites around Lake Kinneret (The Sea of Galilee), collected every 2-4 weeks for about 5 years during 1995-2002 showed irregular fluctuations punctuated by aperiodic outbursts of variable magnitude. Because of the haphazard nature of fecal contamination and large intervals between successive counts, these patterns were described by probabilistic models, based on the truncated Laplace or Extreme Value distribution. Their applicability was tested by comparing the predicted frequencies of counts exceeding different levels calculated from the first half of each record with those actually observed in its second half. Despite the records imperfections and minor violations of the underlying models' assumptions, there was a reasonable agreement between the estimated and actual frequencies. This demonstrated that it is possible to translate the irregular fluctuation pattern into a set of probabilities of future high counts. In principle, such probabilities can be used to quantify the water's fecal contamination pattern and as a tool to assess the efficacy of preventive measures to reduce it.

  16. Formal Specification and Analysis of Danish and Irish Ballot Counting Algorithms

    DEFF Research Database (Denmark)

    Cochran, Dermot

    There are many valid arguments both for and against the use of electronic voting in real world elections. My thesis is that a verification-centric software implementation of a particular algorithm for counting of ballots can be proven correct or shown to be incorrect by an appropriate combination...

  17. Reticulocyte count

    Science.gov (United States)

    ... radiation therapy, or infection) Cirrhosis of the liver Anemia caused by low iron levels, or low levels of vitamin B12 or folate Chronic kidney disease Reticulocyte count may be higher during pregnancy.

  18. Application of patch test in meshless analysis of continuously non-homogeneous piezoelectric circular plate

    Directory of Open Access Journals (Sweden)

    Staňák P.

    2013-06-01

    Full Text Available Proposed paper presents application of the patch test for meshless analysis of piezoelectric circular plate with functionally graded material properties. Functionally graded materials (FGM are the special class of composite materials with continuous variation of volume fraction of constituents in predominant direction. Patch test analysis is an important tool in numerical methods for addressing the convergence. Meshless local Petrov-Galerkin (MLPG method together with moving least-squares (MLS approximation scheme is applied in the analysis. No finite elements are required for approximation or integration of unknown quantities. Circular plate is considered as a 3-D axisymmetric piezoelectric solid. Considering the axial symmetry, the problem is reduced to a 2-dimensinal one. Displacement and electric potential fields are prescribed on the outer boundaries in order to reach the state of constant stress field inside the considered plate as required by the patch test and the governing equations. Values of prescribed mechanical and electrical fields must be determined in order to comply with applied FGM gradation rule. Convergence study is performed to assess the considered meshless approach and several conclusions are finally presented.

  19. -ree vibration analysis of cracked thin plates by quasi-convex coupled isogeometric-meshfree method

    Institute of Scientific and Technical Information of China (English)

    Hanjie ZHANG[1,2; JunzhaoWU[1,2; Dongdong WANG[1,2

    2015-01-01

    The free vibration analysis of cracked thin plates via a quasi-convex coupled isogeometric-meshfree method is presented. This formulation employs the consistently coupled isogeometric-meshfree strategy where a mixed basis vector of the convex B-splines is used to impose the consistency conditions throughout the whole problem domain. Meanwhile, the rigid body modes related to the mixed basis vector and reproducing conditions are also discussed. The mixed basis vector simultaneously offers the consistent isogeometric-meshfree coupling in the coupled region and the quasi-convex property for the meshfree shape functions in the meshfree region, which is particularly attractive for the vibration analysis. The quasi-convex meshfree shape functions mimic the isogeometric basis function as well as offer the meshfree nodal arrangement flexibility. Subsequently, this approach is exploited to study the free vibration analysis of cracked plates, in which the plate geometry is exactly represented by the isogeometric basis functions, while the cracks are discretized by meshfree nodes and highly smoothing approximation is invoked in the rest of the problem domain. The efficacy of the present method is illustrated through several numerical examples.

  20. Childhood trauma and personality disorder criterion counts: a co-twin control analysis.

    Science.gov (United States)

    Berenz, Erin C; Amstadter, Ananda B; Aggen, Steven H; Knudsen, Gun Peggy; Reichborn-Kjennerud, Ted; Gardner, Charles O; Kendler, Kenneth S

    2013-11-01

    Correlational studies consistently report relationships between childhood trauma (CT) and most personality disorder (PD) criteria and diagnoses. However, it is not clear whether CT is directly related to PDs or whether common familial factors (i.e., shared environment and/or genetic factors) better account for that relationship. The current study used a cotwin control design to examine support for a direct effect of CT on PD criterion counts. Participants were from the Norwegian Twin Registry (N = 2,780), including a subset (n = 898) of twin pairs (449 pairs, 45% monozygotic [MZ]) discordant for CT meeting DSM-IV Posttraumatic Stress Disorder Criterion A. All participants completed the Norwegian version of the Structured Interview for DSM-IV Personality. Significant associations between CT and all PD criterion counts were detected in the general sample; however, the magnitude of observed effects was small, with CT accounting for no more than approximately 1% of variance in PD criterion counts. A significant, yet modest, interactive effect was detected for sex and CT on Schizoid and Schizotypal PD criterion counts, with CT being related to these disorders among women but not men. After common familial factors were accounted for in the discordant twin sample, CT was significantly related to Borderline and Antisocial PD criterion counts, but no other disorders; however, the magnitude of observed effects was quite modest (r2 = .006 for both outcomes), indicating that the small effect observed in the full sample is likely better accounted for by common genetic and/or environmental factors. CT does not appear to be a key factor in PD etiology.

  1. Uncertainty Evaluation for Enumeration of Coliforms with Plate Counting Method in Food%食品中大肠菌群平板计数法不确定度的评定

    Institute of Scientific and Technical Information of China (English)

    何晓玲

    2014-01-01

    采用GB 4789.3-2010《食品安全国家标准食品微生物学检验大肠菌群计数》第二法大肠菌群平板计数法进行检验,建立数学模型,对测量不确定度的来源进行分析,建立了简明的食品中大肠菌群平板计数法检测结果的不确定度评定方法,实验室的扩展不确定度为0.050,适用于食品中大肠菌群平板计数法检验的不确定度评定。%According to GB 4789.3-2010 National food safety standard Food microbiological examination Enumeration of coliforms to inspection, established mathematical model, analyzed the sources of the uncertainty of measurement, established a concise method for uncertainty evaluation for plate counting method in food. the expanded uncertainty of laboratory was 0.050. The method is suitable for the uncertainty assessment of the coliform group in food inspection .

  2. Observations of the Hubble Deep Field with the Infrared Space Observatory .3. Source counts and P(D) analysis

    DEFF Research Database (Denmark)

    Oliver, S.J.; Goldschmidt, P.; Franceschini, A.

    1997-01-01

    We present source counts at 6.7 and 15 mu m from our maps of the Hubble Deep Field (HDF) region, reaching 38.6 mu Jy at 6.7 mu m and 255 mu Jy at 15 mu m. These are the first ever extragalactic number counts to be presented at 6.7 mu m, and are three decades fainter than IRAS at 12 mu m. Both sou...... is ruled out at > 3 sigma, while two models which fit the steep IRAS 60-mu m counts are acceptable. This provides important confirmation of the strong evolution seen in IRAS surveys. One of these models can then be ruled out from the 6.7-mu M data....... source counts and a P(D) analysis suggest that we have reached the Infrared Space Observatory (ISO) confusion limit at 15 mu m: this will have important implications for future space missions. These data provide an excellent reference point for other ongoing ISO surveys. A no-evolution model at 15 mu m...

  3. Analysis of geomagnetic storm variations and count-rate of cosmic ray muons recorded at the Brazilian southern space observatory

    Energy Technology Data Exchange (ETDEWEB)

    Frigo, Everton [University of Sao Paulo, USP, Institute of Astronomy, Geophysics and Atmospheric Sciences, IAG/USP, Department of Geophysics, Sao Paulo, SP (Brazil); Savian, Jairo Francisco [Space Science Laboratory of Santa Maria, LACESM/CT, Southern Regional Space Research Center, CRS/INPE, MCT, Santa Maria, RS (Brazil); Silva, Marlos Rockenbach da; Lago, Alisson dal; Trivedi, Nalin Babulal [National Institute for Space Research, INPE/MCT, Division of Space Geophysics, DGE, Sao Jose dos Campos, SP (Brazil); Schuch, Nelson Jorge, E-mail: efrigo@iag.usp.br, E-mail: savian@lacesm.ufsm.br, E-mail: njschuch@lacesm.ufsm.br, E-mail: marlos@dge.inpe.br, E-mail: dallago@dge.inpe.br, E-mail: trivedi@dge.inpe.br [Southern Regional Space Research Center, CRS/INPE, MCT, Santa Maria, RS (Brazil)

    2007-07-01

    An analysis of geomagnetic storm variations and the count rate of cosmic ray muons recorded at the Brazilian Southern Space Observatory -OES/CRS/INPE-MCT, in Sao Martinho da Serra, RS during the month of November 2004, is presented in this paper. The geomagnetic measurements are done by a three component low noise fluxgate magnetometer and the count rates of cosmic ray muons are recorded by a muon scintillator telescope - MST, both instruments installed at the Observatory. The fluxgate magnetometer measures variations in the three orthogonal components of Earth magnetic field, H (North-South), D (East-West) and Z (Vertical), with data sampling rate of 0.5 Hz. The muon scintillator telescope records hourly count rates. The arrival of a solar disturbance can be identified by observing the decrease in the muon count rate. The goal of this work is to describe the physical morphology and phenomenology observed during the geomagnetic storm of November 2004, using the H component of the geomagnetic field and vertical channel V of the multi-directional muon detector in South of Brazil. (author)

  4. EVALUATION OF METHODS OF ANALYSIS TO DETERMINE THE SOMATIC CELL COUNT IN RAW MILK, KEPT IN THE COOLING TANK

    Directory of Open Access Journals (Sweden)

    Manoel Pereira Neto

    2014-06-01

    Full Text Available We analyzed the quality of raw milk from eight dairy property in Rio Grande do Norte, Brazil, stored in a cooling tank, in order to evaluate methods for determining somatic cell counts (SCC. The Somaticell® kit and a portable Direct Cell Counter (DCC were compared with each other and with the MilkoScanTM FT+ (FOSS Denmark, which uses Fourier Transform Infrared Spectroscopy (FTIS. Direct cell counter data were processed for somatic cell scores (log-transformed somatic cell count and analyzed with the SAS®, Statistical Analysis System. Comparison of means and correlation of somatic cell scores were conducted using Pearson’s correlation coefficient and the Tukey Test at 1%. No significant difference was observed for comparison of means. The correlation between somatic cell scores was significant, that is, 0.907 and 0.876 between the MilkoScanTM FT + and the Somaticell® kit and Direct Cell Count (DCC respectively, and 0.943 between the Somaticell® kit and Direct Cell Count (DCC. The methods can be recommended for monitoring the quality of raw milk kept in a cooling tank in the production unit.

  5. Observations of the Hubble Deep Field with the Infrared Space Observatory; 3, Source counts and P(D) analysis

    CERN Document Server

    Oliver, S

    1997-01-01

    We present source counts at 6.7 micron and 15 micron from our maps of the Hubble Deep Field region, reaching 38.6 microJy at 6.7 micron and 255 microJy at 15 micron. These are the first ever extra-galactic number counts to be presented at 6.7 micron and are 3 decades fainter than IRAS at 12 micron. Both source counts and a P(D) analysis suggest we have reached the ISO confusion limit at 15 micron: this will have important implications for future space missions. These data provide an excellent reference point for other ongoing ISO surveys. A no-evolution model at 15 micron is ruled out at >3 sigma, while two models which fit the steep IRAS 60 micron counts are acceptable. This provides important confirmation of the strong evolution seen in IRAS surveys. One of these models can then be ruled out from the 6.7 micron data.

  6. High Reproducibility of ELISPOT Counts from Nine Different Laboratories

    DEFF Research Database (Denmark)

    Sundararaman, Srividya; Karulin, Alexey Y; Ansari, Tameem

    2015-01-01

    laboratories. We plated PBMC at four different concentrations, 24 replicates each, in an IFN-γ ELISPOT assay with HCMV pp65 antigen. The ELISPOT plate, and an image file of the plate was counted in nine different laboratories using ImmunoSpot® Analyzers by (A) Basic Count™ relying on subjective counting...

  7. Health care utilization among Medicare-Medicaid dual eligibles: a count data analysis

    Directory of Open Access Journals (Sweden)

    Shin Jaeun

    2006-04-01

    Full Text Available Abstract Background Medicare-Medicaid dual eligibles are the beneficiaries of both Medicare and Medicaid. Dual eligibles satisfy the eligibility conditions for Medicare benefit. Dual eligibles also qualify for Medicaid because they are aged, blind, or disabled and meet the income and asset requirements for receiving Supplement Security Income (SSI assistance. The objective of this study is to explore the relationship between dual eligibility and health care utilization among Medicare beneficiaries. Methods The household component of the nationally representative Medical Expenditure Panel Survey (MEPS 1996–2000 is used for the analysis. Total 8,262 Medicare beneficiaries are selected from the MEPS data. The Medicare beneficiary sample includes individuals who are covered by Medicare and do not have private health insurance during a given year. Zero-inflated negative binomial (ZINB regression model is used to analyse the count data regarding health care utilization: office-based physician visits, hospital inpatient nights, agency-sponsored home health provider days, and total dental visits. Results Dual eligibility is positively correlated with the likelihood of using hospital inpatient care and agency-sponsored home health services and the frequency of agency-sponsored home health days. Frequency of dental visits is inversely associated with dual eligibility. With respect to racial differences, dually eligible Afro-Americans use more office-based physician and dental services than white duals. Asian duals use more home health services than white duals at the 5% statistical significance level. The dual eligibility programs seem particularly beneficial to Afro-American duals. Conclusion Dual eligibility has varied impact on health care utilization across service types. More utilization of home healthcare among dual eligibles appears to be the result of delayed realization of their unmet healthcare needs under the traditional Medicare-only program

  8. Validation of the sperm class analyser CASA system for sperm counting in a busy diagnostic semen analysis laboratory.

    Science.gov (United States)

    Dearing, Chey G; Kilburn, Sally; Lindsay, Kevin S

    2014-03-01

    Sperm counts have been linked to several fertility outcomes making them an essential parameter of semen analysis. It has become increasingly recognised that Computer-Assisted Semen Analysis (CASA) provides improved precision over manual methods but that systems are seldom validated robustly for use. The objective of this study was to gather the evidence to validate or reject the Sperm Class Analyser (SCA) as a tool for routine sperm counting in a busy laboratory setting. The criteria examined were comparison with the Improved Neubauer and Leja 20-μm chambers, within and between field precision, sperm concentration linearity from a stock diluted in semen and media, accuracy against internal and external quality material, assessment of uneven flow effects and a receiver operating characteristic (ROC) analysis to predict fertility in comparison with the Neubauer method. This work demonstrates that SCA CASA technology is not a standalone 'black box', but rather a tool for well-trained staff that allows rapid, high-number sperm counting providing errors are identified and corrected. The system will produce accurate, linear, precise results, with less analytical variance than manual methods that correlate well against the Improved Neubauer chamber. The system provides superior predictive potential for diagnosing fertility problems.

  9. Biomechanical Analysis of Implanted Clavicle Hook Plates With Different Implant Depths and Materials in the Acromioclavicular Joint: A Finite Element Analysis Study.

    Science.gov (United States)

    Lee, Cheng-Hung; Shih, Cheng-Min; Huang, Kui-Chou; Chen, Kun-Hui; Hung, Li-Kun; Su, Kuo-Chih

    2016-11-01

    Clinical implantation of clavicle hook plates is often used as a treatment for acromioclavicular joint dislocation. However, it is not uncommon to find patients that have developed acromion osteolysis or had peri-implant fracture after hook plate fixation. With the aim of preventing complications or fixation failure caused by implantation of inappropriate clavicle hook plates, the present study investigated the biomechanics of clavicle hook plates made of different materials and with different hook depths in treating acromioclavicular joint dislocation, using finite element analysis (FEA). This study established four parts using computer models: the clavicle, acromion, clavicle hook plate, and screws, and these established models were used for FEA. Moreover, implantations of clavicle hook plates made of different materials (stainless steel and titanium alloy) and with different depths (12, 15, and 18 mm) in patients with acromioclavicular joint dislocation were simulated in the biomechanical analysis. The results indicate that deeper implantation of the clavicle hook plate reduces stress on the clavicle, and also reduces the force applied to the acromion by the clavicle hook plate. Even though a clavicle hook plate made of titanium alloy (a material with a lower Young's modulus) reduces the force applied to the acromion by the clavicle hook plate, slightly higher stress on the clavicle may occur. The results obtained in this study provide a better reference for orthopedic surgeons in choosing different clavicle hook plates for surgery. Copyright © 2016 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.

  10. Crack Imaging and Quantification in Aluminum Plates with Guided Wave Wavenumber Analysis Methods

    Science.gov (United States)

    Yu, Lingyu; Tian, Zhenhua; Leckey, Cara A. C.

    2015-01-01

    Guided wavefield analysis methods for detection and quantification of crack damage in an aluminum plate are presented in this paper. New wavenumber components created by abrupt wave changes at the structural discontinuity are identified in the frequency-wavenumber spectra. It is shown that the new wavenumbers can be used to detect and characterize the crack dimensions. Two imaging based approaches, filter reconstructed imaging and spatial wavenumber imaging, are used to demonstrate how the cracks can be evaluated with wavenumber analysis. The filter reconstructed imaging is shown to be a rapid method to map the plate and any existing damage, but with less precision in estimating crack dimensions; while the spatial wavenumber imaging provides an intensity image of spatial wavenumber values with enhanced resolution of crack dimensions. These techniques are applied to simulated wavefield data, and the simulation based studies show that spatial wavenumber imaging method is able to distinguish cracks of different severities. Laboratory experimental validation is performed for a single crack case to confirm the methods' capabilities for imaging cracks in plates.

  11. Stochastic finite element method for random harmonic analysis of composite plates with uncertain modal damping parameters

    Science.gov (United States)

    Sepahvand, K.

    2017-07-01

    Damping parameters of fiber-reinforced composite possess significant uncertainty due to the structural complexity of such materials. Considering the parameters as random variables, this paper uses the generalized polynomial chaos (gPC) expansion to capture the uncertainty in the damping and frequency response function of composite plate structures. A spectral stochastic finite element formulation for damped vibration analysis of laminate plates is employed. Experimental modal data for samples of plates is used to identify and realize the range and probability distributions of uncertain damping parameters. The constructed gPC expansions for the uncertain parameters are used as inputs to a deterministic finite element model to realize random frequency responses on a few numbers of collocation points generated in random space. The realizations then are employed to estimate the unknown deterministic functions of the gPC expansion approximating the responses. Employing modal superposition method to solve harmonic analysis problem yields an efficient sparse gPC expansion representing the responses. The results show while the responses are influenced by the damping uncertainties at the mid and high frequency ranges, the impact in low frequency modes can be safely ignored. Utilizing a few random collocation points, the method indicates also a very good agreement compared to the sampling-based Monte Carlo simulations with large number of realizations. As the deterministic finite element model serves as black-box solver, the procedure can be efficiently adopted to complex structural systems with uncertain parameters in terms of computational time.

  12. Using Plate Finite Elements for Modeling Fillets in Design, Optimization, and Dynamic Analysis

    Science.gov (United States)

    Brown, A. M.; Seugling, R. M.

    2003-01-01

    A methodology has been developed that allows the use of plate elements instead of numerically inefficient solid elements for modeling structures with 90 degree fillets. The technique uses plate bridges with pseudo Young's modulus (Eb) and thickness (tb) values placed between the tangent points of the fillets. These parameters are obtained by solving two nonlinear simultaneous equations in terms of the independent variables rlt and twallt. These equations are generated by equating the rotation at the tangent point of a bridge system with that of a fillet, where both rotations are derived using beam theory. Accurate surface fits of the solutions are also presented to provide the user with closed-form equations for the parameters. The methodology was verified on the subcomponent level and with a representative filleted structure, where the technique yielded a plate model exhibiting a level of accuracy better than or equal to a high-fidelity solid model and with a 90-percent reduction in the number of DOFs. The application of this method for parametric design studies, optimization, and dynamic analysis should prove extremely beneficial for the finite element practitioner. Although the method does not attempt to produce accurate stresses in the filleted region, it can also be used to obtain stresses elsewhere in the structure for preliminary analysis. A future avenue of study is to extend the theory developed here to other fillet geometries, including fillet angles other than 90 and multifaceted intersections.

  13. Stress Analysis and Optimization of a Piaggio Ape Clutch Plate with different Friction Materials

    Directory of Open Access Journals (Sweden)

    B.Sreedher

    2014-11-01

    Full Text Available clutch plate is one of the important part in the power transmission systems. Good design of clutch provides better engine performance. Clutch is device which is used to engage or disengage of gears and it transfers the rotary motion of one shaft to the other shaft when desired. In automobiles friction clutches are widely used in power transmission applications. To transmit maximum torque in friction clutches selection of the friction material is one of the important tasks. In this thesis a model of Piaggio Ape clutch plate has been generated in Pro-E Cre0-5 and then imported in ANSYS for power transmission applications. We have conducted structural analysis by varying the friction surfaces material and keeping base material aluminium same. By seeing the results, Comparison is done for both materials to validate better lining material for Piaggio Ape clutch plate by doing analysis on clutch with help of ANSYS software for find out which material is best for the lining of friction surfaces.

  14. Static, free vibration and thermal analysis of composite plates and shells using a flat triangular shell element

    Science.gov (United States)

    Kapania, R. K.; Mohan, P.

    1996-09-01

    Finite element static, free vibration and thermal analysis of thin laminated plates and shells using a three noded triangular flat shell element is presented. The flat shell element is a combination of the Discrete Kirchhoff Theory (DKT) plate bending element and a membrane element derived from the Linear Strain Triangular (LST) element with a total of 18 degrees of freedom (3 translations and 3 rotations per node). Explicit formulations are used for the membrane, bending and membrane-bending coupling stiffness matrices and the thermal load vector. Due to a strong analogy between the induced strain caused by the thermal field and the strain induced in a structure due to an electric field the present formulation is readily applicable for the analysis of structures excited by surface bonded or embedded piezoelectric actuators. The results are presented for (i) static analysis of (a) simply supported square plates under doubly sinusoidal load and uniformly distributed load (b) simply supported spherical shells under a uniformly distributed load, (ii) free vibration analysis of (a) square cantilever plates, (b) skew cantilever plates and (c) simply supported spherical shells; (iii) Thermal deformation analysis of (a) simply supported square plates, (b) simply supported-clamped square plate and (c) simply supported spherical shells. A numerical example is also presented demonstrating the application of the present formulation to analyse a symmetrically laminated graphite/epoxy laminate excited by a layer of piezoelectric polyvinylidene flouride (PVDF). The results presented are in good agreement with those available in the literature.

  15. Elastoplastic analysis for infinite plate with centric crack loaded by two pairs of point shear forces

    Institute of Scientific and Technical Information of China (English)

    ZHOU Xiao-ping; LING Tong-hua

    2005-01-01

    The near crack line analysis method was used to investigate a crack loaded by two pairs of point shear forces in an infinite plate in an elastic-perfectly plastic solid, and the analytical solution was obtained. The solutions include: the unit normal vector of the elastic-plastic boundary near the crack line, the elastic-plastic stress fields near crack line, law that the length of the plastic zone along the crack line is varied with an external loads, and the bearing capacity of an infinite plate with a center crack loaded by two pairs of point shear forces. The results are sufficiently precise near the crack line because the assumptions of the small scale yielding theory have not been made and no other assumption have been taken.

  16. Control design and robustness analysis of a ball and plate system by using polynomial chaos

    Energy Technology Data Exchange (ETDEWEB)

    Colón, Diego [University of São Paulo, Polytechnic School, LAC -PTC, São Paulo (Brazil); Balthazar, José M. [São Paulo State University - Rio Claro Campus, Rio Claro (Brazil); Reis, Célia A. dos [São Paulo State University - Bauru Campus, Bauru (Brazil); Bueno, Átila M.; Diniz, Ivando S. [São Paulo State University - Sorocaba Campus, Sorocaba (Brazil); Rosa, Suelia de S. R. F. [University of Brasilia, Brasilia (Brazil)

    2014-12-10

    In this paper, we present a mathematical model of a ball and plate system, a control law and analyze its robustness properties by using the polynomial chaos method. The ball rolls without slipping. There is an auxiliary robot vision system that determines the bodies' positions and velocities, and is used for control purposes. The actuators are to orthogonal DC motors, that changes the plate's angles with the ground. The model is a extension of the ball and beam system and is highly nonlinear. The system is decoupled in two independent equations for coordinates x and y. Finally, the resulting nonlinear closed loop systems are analyzed by the polynomial chaos methodology, which considers that some system parameters are random variables, and generates statistical data that can be used in the robustness analysis.

  17. Free Vibration Analysis for Dynamic Stiffness Degradation of Cracked Cantilever Plate

    Directory of Open Access Journals (Sweden)

    Oday. I. Abdullah

    2005-01-01

    Full Text Available In the present work a dynamic analysis technique have been developed to investigate and characterize the quantity of elastic module degradation of cracked cantilever plates due to presence of a defect such as surface of internal crack under free vibration. A new generalized technique represents the first step in developing a health monitoring system, the effects of such defects on the modal frequencies has been the main key quantifying the elasticity modulii due to presence any type of un-visible defect. In this paper the finite element method has been used to determine the free vibration characteristics for cracked cantilever plate (internal flaws, this present work achieved by different position of crack. Stiffness reduction in term of elastic material properties is analyzed through a parametric study of crack density factor. Results are given for Young’s modulus and shear modulus variation with respects the vibrational characteristics.

  18. TWO-MODE GALERKIN APPROACH IN DYNAMIC STABILITY ANALYSIS OF VISCOELASTIC PLATES

    Institute of Scientific and Technical Information of China (English)

    张能辉; 程昌钧

    2003-01-01

    The dynamic stability of viscoelastic thin plates with large deflections was investigated by using the largest Liapunov exponent analysis and other numerical and analytical dynamic methods. The material behavior was described in terms of the Boltzmann superposition principle. The Galerkin method was used to simplify the original integropartial-differential model into a two-mode approximate integral model, which further reduced to an ordinary differential model by introducing new variables. The dynamic properties of one-mode and two-mode truncated systems were numerically compared. The influence of viscoelastic properties of the material, the loading amplitude and the initial values on the dynamic behavior of the plate under in-plane periodic excitations was discussed.

  19. Shock Analysis of Water Backed Perforated Plate Subjected to Underwater Explosion

    Science.gov (United States)

    Nandagopan, Obla Ramanandam; Ranjithkumar, Santharam; Nandakumar, Chirayil Gopalakrishnannair

    2016-07-01

    Perforated plates are essential structural components of sonar acoustic domes of the submarines and underwater platforms. The sonar acoustic domes are considered as `water backed condition' for structural analysis. It is of utmost importance to study the structural response of sonar acoustic domes subjected to noncontact underwater explosion since it has wide scope in defence application. It is intended to investigate the free field pressure due to the underwater explosion and structural response of perforated plate using ANSYS LS-DYNA software for plastic explosive kirkee (PEK), Tri Nitro Toluene (TNT) and Composition 4 explosives. The free field pressure from TNT explosion is validated with the Cole's expression available for this explosive. The time history plots for free field pressure, displacement and principal stress are plotted for all the three explosives. The free field pressure is validated with Cole's formula and found 18 % variation for TNT and 14 % for PEK explosive.

  20. Control design and robustness analysis of a ball and plate system by using polynomial chaos

    Science.gov (United States)

    Colón, Diego; Balthazar, José M.; dos Reis, Célia A.; Bueno, Átila M.; Diniz, Ivando S.; de S. R. F. Rosa, Suelia

    2014-12-01

    In this paper, we present a mathematical model of a ball and plate system, a control law and analyze its robustness properties by using the polynomial chaos method. The ball rolls without slipping. There is an auxiliary robot vision system that determines the bodies' positions and velocities, and is used for control purposes. The actuators are to orthogonal DC motors, that changes the plate's angles with the ground. The model is a extension of the ball and beam system and is highly nonlinear. The system is decoupled in two independent equations for coordinates x and y. Finally, the resulting nonlinear closed loop systems are analyzed by the polynomial chaos methodology, which considers that some system parameters are random variables, and generates statistical data that can be used in the robustness analysis.

  1. Dynamic buckling analysis of delaminated composite plates using semi-analytical finite strip method

    Science.gov (United States)

    Ovesy, H. R.; Totounferoush, A.; Ghannadpour, S. A. M.

    2015-05-01

    The delamination phenomena can become of paramount importance when the design of the composite plates is concerned. In the current study, the effect of through-the-width delamination on dynamic buckling behavior of a composite plate is studied by implementing semi-analytical finite strip method. In this method, the energy and work integrations are computed analytically due to the implementation of trigonometric functions. Moreover, the method can lead to converged results with comparatively small number of degrees of freedom. These features have made the method quite efficient. To account for delamination effects, displacement field is enriched by adding appropriate terms. Also, the penetration of the delamination surfaces is prevented by incorporating an appropriate contact scheme into the time response analysis. Some selected results are validated against those available in the literature.

  2. A longitudinal analysis of estimation, counting skills, and mathematical ability across the first school year.

    Science.gov (United States)

    Muldoon, Kevin; Towse, John; Simms, Victoria; Perra, Oliver; Menzies, Victoria

    2013-02-01

    In response to claims that the quality (and in particular linearity) of children's mental representation of number acts as a constraint on number development, we carried out a longitudinal assessment of the relationships between number line estimation, counting, and mathematical abilities. Ninety-nine 5-year-olds were tested on 4 occasions at 3 monthly intervals. Correlations between the 3 types of ability were evident, but while the quality of children's estimations changed over time and performance on the mathematical tasks improved over the same period, changes in one were not associated with changes in the other. In contrast to the earlier claims that the linearity of number representation is potentially a unique contributor to children's mathematical development, the data suggest that this variable is not significantly privileged in its impact over and above simple procedural number skills. We propose that both early arithmetic success and estimating skill are bound closely to developments in counting ability. (c) 2013 APA, all rights reserved.

  3. Analysis of the factors that affect photon counts in Compton scattering.

    Science.gov (United States)

    Luo, Guang; Xiao, Guangyu

    2014-10-30

    Compton scattering has been applied in a variety of fields. The factors that affect Compton scattering have been studied extensively in the literature. However, the factors that affect the measured photon counts in Compton scattering are rarely considered. In this paper, we make a detailed discussion on those factors. First, Compton scattering experiments of some alloy series and powder mixture series are explored. Second, the electron density is researched in terms of atom and lattice constants. Third, the factor of attenuation coefficient is discussed. And then, the active degree of electrons is discussed based on the DFT theory. Lastly, the conclusions are made, that the factors affecting Compton scattering photon counts include mainly electron number density, attenuation coefficient and active degree of electrons.

  4. Effect of Measurement vs. Counting Errors on Parameters' Covariance in Neutron Tomography Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Odyniec, Michał [National Security Technologies, LLC. (NSTec), Mercury, NV (United States); Blair, Jerome J. [National Security Technologies, LLC. (NSTec), Mercury, NV (United States)

    2013-06-13

    We present here a method that estimates the relative effect of the counting uncertainty and of the instrument uncertainty on that of the parameters in a parametric model for neutron time of flight. The final result, obtained independently of calculation of the parameter values from measured data, presents explicitly the ratio of the two uncertainties in terms of the choice, settings, and placement of the detector and the oscilloscope. Consequently, the method can serve as a tool in planning a measurement setup.

  5. Experimental analysis of the auditory detection process on avian point counts

    Science.gov (United States)

    Simons, T.R.; Alldredge, M.W.; Pollock, K.H.; Wettroth, J.M.

    2007-01-01

    We have developed a system for simulating the conditions of avian surveys in which birds are identified by sound. The system uses a laptop computer to control a set of amplified MP3 players placed at known locations around a survey point. The system can realistically simulate a known population of songbirds under a range of factors that affect detection probabilities. The goals of our research are to describe the sources and range of variability affecting point-count estimates and to find applications of sampling theory and methodologies that produce practical improvements in the quality of bird-census data. Initial experiments in an open field showed that, on average, observers tend to undercount birds on unlimited-radius counts, though the proportion of birds counted by individual observers ranged from 81% to 132% of the actual total. In contrast to the unlimited-radius counts, when data were truncated at a 50-m radius around the point, observers overestimated the total population by 17% to 122%. Results also illustrate how detection distances decline and identification errors increase with increasing levels of ambient noise. Overall, the proportion of birds heard by observers decreased by 28 ?? 4.7% under breezy conditions, 41 ?? 5.2% with the presence of additional background birds, and 42 ?? 3.4% with the addition of 10 dB of white noise. These findings illustrate some of the inherent difficulties in interpreting avian abundance estimates based on auditory detections, and why estimates that do not account for variations in detection probability will not withstand critical scrutiny. ?? The American Ornithologists' Union, 2007.

  6. Analytical Round Robin for Elastic-Plastic Analysis of Surface Cracked Plates: Phase I Results

    Science.gov (United States)

    Wells, D. N.; Allen, P. A.

    2012-01-01

    An analytical round robin for the elastic-plastic analysis of surface cracks in flat plates was conducted with 15 participants. Experimental results from a surface crack tension test in 2219-T8 aluminum plate provided the basis for the inter-laboratory study (ILS). The study proceeded in a blind fashion given that the analysis methodology was not specified to the participants, and key experimental results were withheld. This approach allowed the ILS to serve as a current measure of the state of the art for elastic-plastic fracture mechanics analysis. The analytical results and the associated methodologies were collected for comparison, and sources of variability were studied and isolated. The results of the study revealed that the J-integral analysis methodology using the domain integral method is robust, providing reliable J-integral values without being overly sensitive to modeling details. General modeling choices such as analysis code, model size (mesh density), crack tip meshing, or boundary conditions, were not found to be sources of significant variability. For analyses controlled only by far-field boundary conditions, the greatest source of variability in the J-integral assessment is introduced through the constitutive model. This variability can be substantially reduced by using crack mouth opening displacements to anchor the assessment. Conclusions provide recommendations for analysis standardization.

  7. Paper Plate Fractions: The Counting Connection

    Science.gov (United States)

    McCoy, Ann; Barnett, Joann; Stine, Tammy

    2016-01-01

    Without a doubt, fractions prove to be a stumbling block for many children. Researchers have suggested a variety of explanations for why this is the case. The introduction of symbolization and operations before the development of conceptual understanding of fractions, a lack of understanding of the role of the numerator and denominator, and an…

  8. The study of error for analysis in dynamic image from the error of count rates in Nal (Tl) scintillation camera

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Joo Young; Kang, Chun Goo; Kim, Jung Yul; Oh, Ki Baek; Kim, Jae Sam [Dept. of Nuclear Medicine, Severance Hospital, Yonsei University, Seoul (Korea, Republic of); Park, Hoon Hee [Dept. of Radiological Technology, Shingu college, Sungnam (Korea, Republic of)

    2013-12-15

    This study is aimed to evaluate the effect of T{sub 1/2} upon count rates in the analysis of dynamic scan using NaI (Tl) scintillation camera, and suggest a new quality control method with this effects. We producted a point source with '9{sup 9m}TcO{sub 4}- of 18.5 to 185 MBq in the 2 mL syringes, and acquired 30 frames of dynamic images with 10 to 60 seconds each using Infinia gamma camera (GE, USA). In the second experiment, 90 frames of dynamic images were acquired from 74 MBq point source by 5 gamma cameras (Infinia 2, Forte 2, Argus 1). There were not significant differences in average count rates of the sources with 18.5 to 92.5 MBq in the analysis of 10 to 60 seconds/frame with 10 seconds interval in the first experiment (p>0.05). But there were significantly low average count rates with the sources over 111 MBq activity at 60 seconds/frame (p<0.01). According to the second analysis results of linear regression by count rates of 5 gamma cameras those were acquired during 90 minutes, counting efficiency of fourth gamma camera was most low as 0.0064%, and gradient and coefficient of variation was high as 0.0042 and 0.229 each. We could not find abnormal fluctuation in χ{sup 2} test with count rates (p>0.02), and we could find the homogeneity of variance in Levene's F-test among the gamma cameras (p>0.05). At the correlation analysis, there was only correlation between counting efficiency and gradient as significant negative correlation (r=-0.90, p<0.05). Lastly, according to the results of calculation of T{sub 1/2} error from change of gradient with -0.25% to +0.25%, if T{sub 1/2} is relatively long, or gradient is high, the error increase relationally. When estimate the value of 4th camera which has highest gradient from the above mentioned result, we could not see T{sub 1/2} error within 60 minutes at that value. In conclusion, it is necessary for the scintillation gamma camera in medical field to manage hard for the quality of radiation

  9. Buckling Analysis of Angle-ply Composite and Sandwich Plates by Combination of Geometric Stiffness Matrix

    Science.gov (United States)

    Zhen, Wu; Wanji, Chen

    2007-05-01

    Buckling response of angle-ply laminated composite and sandwich plates are analyzed using the global-local higher order theory with combination of geometric stiffness matrix in this paper. This global-local theory completely fulfills the free surface conditions and the displacement and stress continuity conditions at interfaces. Moreover, the number of unknowns in this theory is independent of the number of layers in the laminate. Based on this global-local theory, a three-noded triangular element satisfying C1 continuity conditions has also been proposed. The bending part of this element is constructed from the concept of DKT element. In order to improve the accuracy of the analysis, a method of modified geometric stiffness matrix has been introduced. Numerical results show that the present theory not only computes accurately the buckling response of general laminated composite plates but also predicts the critical buckling loads of soft-core sandwiches. However, the global higher-order theories as well as first order theories might encounter some difficulties and overestimate the critical buckling loads for soft-core sandwich plates.

  10. Dynamical Analysis of Long Fiber-Reinforced Laminated Plates with Elastically Restrained Edges

    Directory of Open Access Journals (Sweden)

    Liz G. Nallim

    2012-01-01

    Full Text Available This paper presents a variational formulation for the free vibration analysis of unsymmetrically laminated composite plates with elastically restrained edges. The study includes a micromechanics approach that allows starting the study considering each layer as constituted by long unidirectional fibers in a continuous matrix. The Mori-Tanaka method is used to predict the mechanical properties of each lamina as a function of the elastic properties of the components and of the fiber volume fraction. The resulting mechanical properties for each lamina are included in a general Ritz formulation developed to analyze the free vibration response of thick laminated anisotropic plates resting on elastic supports. Comprehensive numerical examples are computed to validate the present method, and the effects of the different mechanical and geometrical parameters on the dynamical behavior of different laminated plates are shown. New results for general unsymmetrical laminates with elastically restrained edges are also presented. The analytical approximate solution obtained in this paper can also be useful as a basis to deal with optimization problems under, for instance, frequency constraints.

  11. Preliminary Stress Analysis of an IHX Tube Support Plate in Prototype SFR

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Sung Kyun; Koo, Gyeong Hoi [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2013-10-15

    In this paper, the structural integrity about the conceptual design of IHX tube support plate was reviewed and the design should be changed because of its high stress concentration at the outer rim area. For reducing its maximum stress, two alternatives were proposed and reviewed for the structural integrity point of view. In both proposing support designs, the maximum stress decreases up to the stress design limit. Tube support plates (TSPs) of the intermediate heat exchanger (IHX) in Prototype GenIV Sodium Cooled Fast Reactor (PGSFR) act to horizontally support IHX tubes against hydraulic loadings and they have numerous flow holes where a primary sodium flows downward and secondary sodium flows upward. Due to its many penetrations, its geometric shape is quite complex and structurally its integrity is quite weaker than other parts. In this study, we investigated the structural integrity of the conceptually designed IHX tube support plate. In addition, TSP's supporting concepts were proposed to increase its structural integrity, and confirmed its integrity by using a finite element analysis.

  12. Experimental fuel cell performance analysis under different operating conditions and bipolar plate designs

    Energy Technology Data Exchange (ETDEWEB)

    Iranzo, Alfredo; Pino, Javier; Rosa, Felipe [Energy Engineering Department, School of Engineering, University of Seville, Camino de los Descubrimientos s/n, 41092 Seville (Spain); Munoz, Miguel; Lopez, Eduardo [INTA - National Institute for Aerospace Technology, Ctra. San Juan del Puerto-Matalascanas km 33, 21130 Mazagon (Huelva) (Spain)

    2010-10-15

    This work presents experimental performance results for a 50 cm{sup 2} Polymer Electrolyte Membrane (PEM) Fuel Cell, including polarization curves and Electrochemical Impedance Spectroscopy (EIS) analysis of the Fuel Cell. EIS results were used for the determination of the cell ohmic resistance as well as charge transfer resistances under different operating conditions. Different combinations of operating conditions and bipolar plate designs were analysed. In particular, the effect of the cathode oxygen concentration, reactant gases humidification, and bipolar plate (BP) design were assessed. Butler-Volmer (BV) kinetic parameters such as the charge transfer coefficient were also determined from Tafel plots. The electronic contact resistances were measured for both Bipolar Plate designs, and the membrane protonic resistances were calculated. Its dependence on the BP flow field design and operating conditions is addressed. The results obtained in this work are aimed both at gaining insight into the fundamental processes determining the fuel cell performance, and at determining parameters needed for Computational Fuel Cell Dynamics (CFCD) numerical simulations. (author)

  13. Understanding Blood Counts

    Science.gov (United States)

    ... Lab and Imaging Tests Understanding Blood Counts Understanding Blood Counts Understanding Blood Counts SHARE: Print Glossary Blood cell counts give ... your blood that's occupied by red cells. Normal Blood Counts Normal blood counts fall within a range ...

  14. White Blood Cell Count

    Science.gov (United States)

    ... limited. Home Visit Global Sites Search Help? White Blood Cell Count Share this page: Was this page helpful? ... Count; Leukocyte Count; White Count Formal name: White Blood Cell Count Related tests: Complete Blood Count , Blood Smear , ...

  15. Golgi analysis of tangential neurons in the lobula plate of Drosophila melanogaster

    Indian Academy of Sciences (India)

    K P Rajashekhar; V R Shamprasad

    2004-03-01

    The lobula plate (LP), which is the third order optic neuropil of flies, houses wide-field neurons which are exquisitely sensitive to motion. Among Diptera, motion-sensitive neurons of larger flies have been studied at the anatomical and physiological levels. However, the neurons of Drosophila lobula plate are relatively less explored. As Drosophila permits a genetic analysis of neural functions, we have analysed the organization of lobula plate of Drosophila melanogaster. Neurons belonging to eight anatomical classes have been observed in the present study. Three neurons of the horizontal system (HS) have been visualized. The HS north (HSN) neuron, occupying the dorsal lobula plate is stunted in its geometry compared to that of larger flies. Associated with the HS neurons, thinner horizontal elements known as h-cells have also been visualized in the present study. Five of the six known neurons of the vertical system (VS) have been visualized. Three additional neurons in the proximal LP comparable in anatomy to VS system have been stained. We have termed them as additional VS (AVS)-like neurons. Three thinner tangential cells that are comparable to VS neurons, which are elements of twin vertical system (tvs); and two cells with wide dendritic fields comparable to CH neurons of Diptera have been also observed. Neurons comparable to VS cells but with ‘tufted’ dendrites have been stained. The HSN and VS1-VS2 neurons are dorsally stunted. This is possibly due to the shape of the compound eye of Drosophila which is reduced in the fronto-dorsal region as compared to larger flies.

  16. Stiffness Analysis of Nail-Plate Joints Subjected to Short-Term Loads

    DEFF Research Database (Denmark)

    Nielsen, Jacob

    with beams connected in joints with glue or a mechanical fastener. The types of mechanical fasteners are: nails, staples, bolts, dowels, screws and nail-plates. Bolts and dowels are generally applied to joints in solid structures, and the other fasteners are used in all kinds of light structures. Especially...... types of plates. There are two main types of nail-plates: steel plates perforated with holes in which separate nails are used and steel plates perforated by a stamping machine, so the nails are made from the plate, see figur 1.2 on page 7. This type is sometimes called "punching metal plate...

  17. Counting Populations

    Science.gov (United States)

    Damonte, Kathleen

    2004-01-01

    Scientists use sampling to get an estimate of things they cannot easily count. A population is made up of all the organisms of one species living together in one place at the same time. All of the people living together in one town are considered a population. All of the grasshoppers living in a field are a population. Scientists keep track of the…

  18. Comparative analysis of amniotic fluid lamellar body count and foam stability test as indices of fetal lung maturity

    Directory of Open Access Journals (Sweden)

    Višnjevac Nemanja

    2010-01-01

    Full Text Available Introduction. Respiratory distress syndrome of the newborn caused by the fetal lung immaturity is a very serious clinical problem. Different tests of prenatal analysis of amniotic fluid, such as lamellar body count and Clements’ test, are available for predicting the fetal lung maturity. Material and methods. A prospective clinical study was conducted on amniotic fluid samples from 2005 to 2006. The amniotic fluid samples were obtained at the gestational age of 30 to 42 weeks and collected by vaginal amniotomy, amniotomy during Caesarean section and 72 hours before the delivery by amniocentesis. A haematology analyzer (Nikon-Kohden® was used to determine the lamellar body counts. Clements’ test involved adding an equal volume of 96% ethanol to the multiple amniotic fluid volume (1:2, 1:4, 1:16, 1:32, followed by shaking and noting the presence of ring of bubbles. After the delivery, we compared the lamellar body count results and Clements’ test and the outcome of pregnancies, primarily the development of respiratory distress syndrome. The most specific lamellar body cutoffs for maturity and immaturity were determined according to receiver operating characteristic curves. Results and Discussion. Out of 232 amniotic fluid samples which were tested, 112 samples were collected after vaginal amniotomy, 88 during the Caesarean delivery and 32 samples by amniocentesis. The overall incidence of respiratory distress syndrome was 14.6%. Receiver operating characteristic curves were used to identify cutoff points for the test. We found that both tests are good screening tests for predicting the fetal lung maturity with the area under the curve of 0.782 in Clements’ test and 0.751 in the lamellar body count. Clements’ cutoff 2 with sensitivity of 67.6% and specificity of 72.2%, proved best in the prediction of the fetal lung maturity. The lamellar body count cutoff of 42x10³/μl had the sensitivity of 82.4% and specificity of 64.6% in predicting

  19. Galaxy Number Counts in the Subaru Deep Field Multi-band Analysis in a Hierarchical Galaxy Formation Model

    CERN Document Server

    Nagashima, M; Totani, T; Gouda, N

    2002-01-01

    Number counts of galaxies are re-analyzed using a semi-analytic model (SAM) of galaxy formation based on the hierarchical clustering scenario. Faint galaxies in the Subaru Deep Field (SDF) and the Hubble Deep Field (HDF) are compared with our model galaxies. We have determined the astrophysical parameters in the SAM that reproduce observations of nearby galaxies, and used them to predict the number counts and redshifts of faint galaxies for three cosmological models, the standard cold dark matter (CDM) universe, a flat lambda-CDM, and an open CDM. The novelty of our SAM analysis is the inclusion of selection effects arising from the cosmological dimming of surface brightness of high-z galaxies, and from the absorption of visible light by internal dust and intergalactic HI clouds. As was found in our previous work, in which the UV/optical HDF galaxies were compared with our model galaxies, we find that our SAM reproduces counts of near-IR SDF galaxies in low-density models, and that the standard CDM universe i...

  20. The analysis of incontinence episodes and other count data in patients with overactive bladder by Poisson and negative binomial regression.

    Science.gov (United States)

    Martina, R; Kay, R; van Maanen, R; Ridder, A

    2015-01-01

    Clinical studies in overactive bladder have traditionally used analysis of covariance or nonparametric methods to analyse the number of incontinence episodes and other count data. It is known that if the underlying distributional assumptions of a particular parametric method do not hold, an alternative parametric method may be more efficient than a nonparametric one, which makes no assumptions regarding the underlying distribution of the data. Therefore, there are advantages in using methods based on the Poisson distribution or extensions of that method, which incorporate specific features that provide a modelling framework for count data. One challenge with count data is overdispersion, but methods are available that can account for this through the introduction of random effect terms in the modelling, and it is this modelling framework that leads to the negative binomial distribution. These models can also provide clinicians with a clearer and more appropriate interpretation of treatment effects in terms of rate ratios. In this paper, the previously used parametric and non-parametric approaches are contrasted with those based on Poisson regression and various extensions in trials evaluating solifenacin and mirabegron in patients with overactive bladder. In these applications, negative binomial models are seen to fit the data well.

  1. Reference analysis of the signal + background model in counting experiments II. Approximate reference prior

    Science.gov (United States)

    Casadei, D.

    2014-10-01

    The objective Bayesian treatment of a model representing two independent Poisson processes, labelled as ``signal'' and ``background'' and both contributing additively to the total number of counted events, is considered. It is shown that the reference prior for the parameter of interest (the signal intensity) can be well approximated by the widely (ab)used flat prior only when the expected background is very high. On the other hand, a very simple approximation (the limiting form of the reference prior for perfect prior background knowledge) can be safely used over a large portion of the background parameters space. The resulting approximate reference posterior is a Gamma density whose parameters are related to the observed counts. This limiting form is simpler than the result obtained with a flat prior, with the additional advantage of representing a much closer approximation to the reference posterior in all cases. Hence such limiting prior should be considered a better default or conventional prior than the uniform prior. On the computing side, it is shown that a 2-parameter fitting function is able to reproduce extremely well the reference prior for any background prior. Thus, it can be useful in applications requiring the evaluation of the reference prior for a very large number of times.

  2. Scaling analysis of high-frequency time series of gamma-ray counts

    Science.gov (United States)

    Barbosa, Susana; Azevedo, Eduardo

    2017-04-01

    Gamma radiation is being monitored in a dedicated campaign set-up at the Eastern North Atlantic (ENA) facility located in the Graciosa island (Azores), a fixed site of the Atmospheric Radiation Measurement programme (ARM), established and supported by the Department of Energy (DOE) of the United States of America with the collaboration of the Government of the Autonomous Region of the Azores and University of the Azores. The temporal variability of gamma radiation is mainly determined by the time-varying concentration of radon progeny, which in turn is influenced by meteorological conditions and precipitation scavenging. The resulting time series of high-frequency (1-minute) gamma-ray counts displays therefore a complex temporal structure on multiple time scales, including long-range dependent behavior. This work addresses the scaling properties of the time series of gamma-ray counts from the ENA site (data freely available from the ARM data archive) using both wavelet and model-based methods for the estimation of the scaling exponent. The time series is dominated by sharp peaks associated with events of strong precipitation. The effect of these peaks on the estimation of the scaling exponent, as well as the effect of temporal aggregation (1-minute versus 15-minute aggregated data) is further addressed.

  3. Triangular Differential Quadrature for Bending Analysis of Reissner Plates with Curved Boundaries

    Institute of Scientific and Technical Information of China (English)

    华永霞; 钟宏志

    2003-01-01

    The recently proposed concept of the triangular differential quadrature method (TDQM) is applied to the bending analysis of Reissner plates with various curvilinear geometries subjected to various combinations of boundary conditions. A unit isosceles right triangle is used as the standard triangle for all the derivatives expressed using the triangular differential quadrature rule. Geometric transformations are introduced using basis functions to determine the weighting coefficients for the triangular differential quadrature to map an arbitrary curvilinear triangle into the standard triangle. The triangular differential quadrature method provides good accuracy and rapid convergence relative to other available exact and numerical results.

  4. The Estimation of Vibrational Energy of Two Coupled (Welded) Plates Using Statistical Energy Analysis

    OpenAIRE

    2011-01-01

    This paper deals with a method called Statistical Energy Analysis that can be applied to the mechanical and acoustical systems like buildings, bridges and aircrafts …etc. S.E.A as a tool can be applied to the resonant systems in the circumstances of high frequency or/and complex structure». The parameters of S.E.A such as coupling loss factor, internal loss factor, modal density and input power are clarified in this work ; coupled plate sub-systems and explanations are presented for these par...

  5. Analysis of thin plates with holes by using exact geometrical representation within XFEM

    Science.gov (United States)

    Perumal, Logah; Tso, C.P.; Leng, Lim Thong

    2016-01-01

    This paper presents analysis of thin plates with holes within the context of XFEM. New integration techniques are developed for exact geometrical representation of the holes. Numerical and exact integration techniques are presented, with some limitations for the exact integration technique. Simulation results show that the proposed techniques help to reduce the solution error, due to the exact geometrical representation of the holes and utilization of appropriate quadrature rules. Discussion on minimum order of integration order needed to achieve good accuracy and convergence for the techniques presented in this work is also included. PMID:27222749

  6. Systematic analysis of in vitro cell rolling using a multi-well plate microfluidic system.

    Science.gov (United States)

    Levy, Oren; Anandakumaran, Priya; Ngai, Jessica; Karnik, Rohit; Karp, Jeffrey M

    2013-10-16

    A major challenge for cell-based therapy is the inability to systemically target a large quantity of viable cells with high efficiency to tissues of interest following intravenous or intraarterial infusion. Consequently, increasing cell homing is currently studied as a strategy to improve cell therapy. Cell rolling on the vascular endothelium is an important step in the process of cell homing and can be probed in-vitro using a parallel plate flow chamber (PPFC). However, this is an extremely tedious, low throughput assay, with poorly controlled flow conditions. Instead, we used a multi-well plate microfluidic system that enables study of cellular rolling properties in a higher throughput under precisely controlled, physiologically relevant shear flow. In this paper, we show how the rolling properties of HL-60 (human promyelocytic leukemia) cells on P- and E-selectin-coated surfaces as well as on cell monolayer-coated surfaces can be readily examined. To better simulate inflammatory conditions, the microfluidic channel surface was coated with endothelial cells (ECs), which were then activated with tumor necrosis factor-α (TNF-α), significantly increasing interactions with HL-60 cells under dynamic conditions. The enhanced throughput and integrated multi-parameter software analysis platform, that permits rapid analysis of parameters such as rolling velocities and rolling path, are important advantages for assessing cell rolling properties in-vitro. Allowing rapid and accurate analysis of engineering approaches designed to impact cell rolling and homing, this platform may help advance exogenous cell-based therapy.

  7. Thermal Analysis on Butt Welded Aluminium Alloy AA7075 Plate Using FEM

    Directory of Open Access Journals (Sweden)

    M. Pal Pandi

    2014-03-01

    Full Text Available Thermo-mechanical finite element analysis has been performed to assess the residual stress in the butt weld joints of aluminium Alloy AA7075 plates by utilizing the commercial software package ABAQUS. This paper presents an efficient FE technique using equivalent load to precisely predict welding deformations and residual stresses in butt joints. The radial heat flux distribution is considered on the top surface of the weldment. Convective and radiative heat losses are taken into account through boundary conditions for the outward heat flux. Linear FE transient thermal analysis is performed using surface heat source model with Gaussian distribution to compute highest temperature in AA7075 plates. The objective of this project is to simulate the welding process by using the finite element method. After the model is built and verified, the main objective of this project is to study the effects of varying the welding process parameters on the thermo-mechanical responses. In addition to that, the aim of this research is also to find a relationship between welding parameters and the responses of single pass butt welding are evaluated through the finite element analysis. The study of this paper covers the effects of varying heat input, welding speed on the thermo-mechanical responses of the weldment after cooling down to room temperature.

  8. [Analysis of variance of bacterial counts in milk. 1. Characterization of total variance and the components of variance random sampling error, methodologic error and variation between parallel errors during storage].

    Science.gov (United States)

    Böhmer, L; Hildebrandt, G

    1998-01-01

    In contrast to the prevailing automatized chemical analytical methods, classical microbiological techniques are linked with considerable material- and human-dependent sources of errors. These effects must be objectively considered for assessing the reliability and representativeness of a test result. As an example for error analysis, the deviation of bacterial counts and the influence of the time of testing, bacterial species involved (total bacterial count, coliform count) and the detection method used (pour-/spread-plate) were determined in a repeated testing of parallel samples of pasteurized (stored for 8 days at 10 degrees C) and raw (stored for 3 days at 6 degrees C) milk. Separate characterization of deviation components, namely, unavoidable random sampling error as well as methodical error and variation between parallel samples, was made possible by means of a test design where variance analysis was applied. Based on the results of the study, the following conclusions can be drawn: 1. Immediately after filling, the total count deviation in milk mainly followed the POISSON-distribution model and allowed a reliable hygiene evaluation of lots even with few samples. Subsequently, regardless of the examination procedure used, the setting up of parallel dilution series can be disregarded. 2. With increasing storage period, bacterial multiplication especially of psychrotrophs leads to unpredictable changes in the bacterial profile and density. With the increase in errors between samples, it is common to find packages which have acceptable microbiological quality but are already spoiled by the time of the expiry date labeled. As a consequence, a uniform acceptance or rejection of the batch is seldom possible. 3. Because the contamination level of coliforms in certified raw milk mostly lies near the detection limit, coliform counts with high relative deviation are expected to be found in milk directly after filling. Since no bacterial multiplication takes place

  9. Analysis of photon count data from single-molecule fluorescence experiments

    Energy Technology Data Exchange (ETDEWEB)

    Burzykowski, T.; Szubiakowski, J.; Ryden, T

    2003-03-15

    We consider single-molecule fluorescence experiments with data in the form of counts of photons registered over multiple time-intervals. Based on the observation schemes, linking back to works by Dehmelt [Bull. Am. Phys. Soc. 20 (1975) 60] and Cook and Kimble [Phys. Rev. Lett. 54 (1985) 1023], we propose an analytical approach to the data based on the theory of Markov-modulated Poisson processes (MMPP). In particular, we consider maximum-likelihood estimation. The method is illustrated using a real-life dataset. Additionally, the properties of the proposed method are investigated through simulations and compared to two other approaches developed by Yip et al. [J. Phys. Chem. A 102 (1998) 7564] and Molski [Chem. Phys. Lett. 324 (2000) 301].

  10. Performance and capacity analysis of Poisson photon-counting based Iter-PIC OCDMA systems.

    Science.gov (United States)

    Li, Lingbin; Zhou, Xiaolin; Zhang, Rong; Zhang, Dingchen; Hanzo, Lajos

    2013-11-04

    In this paper, an iterative parallel interference cancellation (Iter-PIC) technique is developed for optical code-division multiple-access (OCDMA) systems relying on shot-noise limited Poisson photon-counting reception. The novel semi-analytical tool of extrinsic information transfer (EXIT) charts is used for analysing both the bit error rate (BER) performance as well as the channel capacity of these systems and the results are verified by Monte Carlo simulations. The proposed Iter-PIC OCDMA system is capable of achieving two orders of magnitude BER improvements and a 0.1 nats of capacity improvement over the conventional chip-level OCDMA systems at a coding rate of 1/10.

  11. Mechanical analysis of functionally graded plates based on the input data of metallographic diagrams

    Institute of Scientific and Technical Information of China (English)

    CAO ZhiYuan; TANG ShouGao; CHENG HongMei

    2008-01-01

    A microelement method for scale-span analysis of material microstructure and member macro re-sponse has been proposed. Instead of material parameter input for traditional mechanical analysis, the method is based on the input of metallographic diagram information. In order to express the material microstructure, this method arranges concentrated microelements in ordinary finite element before calculation, and transfers the node degrees of freedom of each microelement into the ones of the same finite element via compatibility conditions. This method can realize direct transition analysis from ma-terial microstructure to macro responses of members, while computation elements and degrees of freedom are equal to those of ordinary FEM. Based on the complicated microstructure diagrams pro-posed by material metallographic diagram, the mechanics responses of functionally graded plates are calculated and 3-D distributions of macro-mechanical variables and identical stress lines tendency on microstructure are given.

  12. Application of Homotopy Analysis Method to the Unsteady Squeezing Flow of a Second-Grade Fluid between Circular Plates

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Rashidi

    2010-01-01

    Full Text Available We investigated an axisymmetric unsteady two-dimensional flow of nonconducting, incompressible second grade fluid between two circular plates. The similarity transformation is applied to reduce governing partial differential equation (PDE to a nonlinear ordinary differential equation (ODE in dimensionless form. The resulting nonlinear boundary value problem is solved using homotopy analysis method and numerical method. The effects of appropriate dimensionless parameters on the velocity profiles are studied. The total resistance to the upper plate has been calculated.

  13. Experiment and simulation analysis of roll-bonded Q235 steel plate

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, G.; Huang, Q.; Zhou, C.; Zhang, Z.; Ma, L.; Wang, X.

    2016-10-01

    Heavy-gauge Q235 steel plate was roll bonded, and the process was simulated using MARC software. Ultrasonic testing results revealed the presence of cracks and lamination defects in an 80-mm clad steel sheet, especially at the head and tail of the steel plate. There were non-uniform ferrite + pearlite microstructures and unbound areas at a bond interface. Through scanning electron microscopy analysis, long cracks and additional inclusions in the cracks were observed at the interface. A fracture analysis revealed non-uniform inclusions that pervaded the interface. Moreover, MARC simulations demonstrated that there was little equivalent strain at the centre of the slab during the first rolling pass. The equivalent centre increased to 0.5 by the fourth rolling pass. Prior to the final pass, the equivalent strain was not consistent across the thickness direction, preventing bonding interfaces from forming consistent deformation and decreasing the residual stress. The initial rolling reduction rate should not be very small (e.g. 5%) as it is averse to the coordination of rolling deformation. Such rolling processes are averse to the rolling bond. (Author)

  14. Analytical Round Robin for Elastic-Plastic Analysis of Surface Cracked Plates, Phase II Results

    Science.gov (United States)

    Allen, P. A.; Wells, D. N.

    2017-01-01

    The second phase of an analytical round robin for the elastic-plastic analysis of surface cracks in flat plates was conducted under the auspices of ASTM Interlaboratory Study 732. The interlaboratory study (ILS) had 10 participants with a broad range of expertise and experience, and experimental results from a surface crack tension test in 4142 steel plate loaded well into the elastic-plastic regime provided the basis for the study. The participants were asked to evaluate a surface crack tension test according to the version of the surface crack initiation toughness testing standard published at the time of the ILS, E2899-13. Data were provided to each participant that represent the fundamental information that would be provided by a mechanical test laboratory prior to evaluating the test result. Overall, the participant’s test analysis results were in good agreement and constructive feedback was received that has resulted in an improved published version of the standard E2899-15.

  15. Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells

    Science.gov (United States)

    Roellig, Daniela; Tan-Cabugao, Johanna; Esaian, Sevan; Bronner, Marianne E

    2017-01-01

    The ‘neural plate border’ of vertebrate embryos contains precursors of neural crest and placode cells, both defining vertebrate characteristics. How these lineages segregate from neural and epidermal fates has been a matter of debate. We address this by performing a fine-scale quantitative temporal analysis of transcription factor expression in the neural plate border of chick embryos. The results reveal significant overlap of transcription factors characteristic of multiple lineages in individual border cells from gastrula through neurula stages. Cell fate analysis using a Sox2 (neural) enhancer reveals that cells that are initially Sox2+ cells can contribute not only to neural tube but also to neural crest and epidermis. Moreover, modulating levels of Sox2 or Pax7 alters the apportionment of neural tube versus neural crest fates. Our results resolve a long-standing question and suggest that many individual border cells maintain ability to contribute to multiple ectodermal lineages until or beyond neural tube closure. DOI: http://dx.doi.org/10.7554/eLife.21620.001 PMID:28355135

  16. Imaging of Cocos Plate Beneath Southern Costa Rica From Receiver Function Analysis

    Science.gov (United States)

    Dzierma, Y.; Thorwart, M.; Rabbel, W.

    2007-12-01

    A transect of 19 seismological broadband stations crossing the Talamanca Mountain Range in Southern Costa Rica was operated from March 2005 to April 2007 as a part of the Collaborative Research Center SFB 574 "Volatiles and Fluids in Subduction Zones". The aim of the seismological subproject A2 was to gain insight into the structure of the Central American subduction zone and possible pathways for fluid migration. Previous studies of active seismics and local seismicity suggested to explain the gap of volcanism in the Talamanca range with the lack of a subducting slab. They assumed that the Cocos Ridge underlies the overriding plate at a shallow dip. In contrast, our receiver function analysis of 322 teleseimic earthquakes is able to image the subducting Cocos Plate down to depths of at least 100 km. The dip angle of the slab closer to the trench is outside the network but appears to be shallow, consistent with former studies. Below 40 km, the dip increases to more than 45 deg. This is supported by accurately located seismicity from a tomography study also performed by our group. Crustal structure could also be resolved by the receiver function analysis in agreement with tomography and active seismic investigations. The existence of the subducting slab poses the question why volcanism stopped 4 Ma ago; several possible scenarios are discussed.

  17. Flow Field and Thermal Analysis of the Divertor Target Plate for HL-2A Tokamak

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    In the initial phase of the physics experiment, the double-null divertor plates used consist of graphite armor tiles, Mo-alloy intermediate layers and Cu-alloy coolant tubes. In the later operating phase, tungsten will be used as armor tiles.A multi-physical field numerical analysis method is used in this paper. Its analysis model reflects more realistically the real divertor structure than other models. Two-dimensional (2D)and three-dimensional (3D) fluid flow field, temperature distribution and thermal stress analyses of the divertor plates are carried out by the ANSYS code. During the physics experimental phase with a heat flux of 1 MW/m2, a coolant velocity of 5.48 m/s, and a thermal stress of 750 kg/cm2,the graphite armor tiles successfully meet the requirements of temperature, thermal stress and sputtering erosion. The tungsten armor will be considered as a second candidate. The result of simulation can be used for upgrading the design parameters of the HL-2A poloidal divertor.

  18. Prognostic value of preoperative absolute lymphocyte count in recurrent hepatocellular carcinoma following thermal ablation: a retrospective analysis.

    Science.gov (United States)

    Li, Xin; Han, Zhiyu; Cheng, Zhigang; Yu, Jie; Yu, Xiaoling; Liang, Ping

    2014-01-01

    To investigate the prognostic value of preoperative absolute lymphocyte count (ALC) in recurrent hepatocellular carcinoma (RHCC) following thermal ablation. We retrospectively analyzed the relationship between preoperative ALC and the clinicopathologic factors and long-term prognosis in 423 RHCC patients who underwent curative thermal ablation. Correlation analysis, receiver operating characteristic (ROC) calculation, Kaplan-Meier curves, and multivariate regression were used for statistical analysis. The median time to recurrence was 12 months for RHCC patients after thermal ablation. On multivariate Cox regression analysis, preoperative ALC was an independent risk factor for cancer recurrence, along with tumor differentiation and α-fetoprotein level. ALC ≥1.64×10(9)/L defined by ROC calculation was associated with prolonged survival (area under the curve 0.741, Pthermal ablation, which suggests that maintaining a high ALC in RHCC patients might improve cancer outcomes.

  19. Analysis of single blow effectiveness in non-uniform parallel plate regenerators

    DEFF Research Database (Denmark)

    Jensen, Jesper Buch; Bahl, Christian Robert Haffenden; Engelbrecht, Kurt;

    2011-01-01

    Non-uniform distributions of plate spacings in parallel plate regenerators have been found to induce loss of performance. In this paper, it has been investigated how variations of three geometric parameters (the aspect ratio, the porosity, and the standard deviation of the plate spacing) affects...... this loss in a single blow model of a parallel-plate regenerator. Simple analytical functions for the magnitude and the time scale of the reduction of performance are presented and compared to numerical results....

  20. Shrinkage Analysis on Thick Plate Part using Response Surface Methodology (RSM

    Directory of Open Access Journals (Sweden)

    Isafiq M.

    2016-01-01

    Full Text Available The work reported herein is about an analysis on the quality (shrinkage on a thick plate part using Response Surface Methodology (RSM. Previous researches showed that the most influential factor affecting the shrinkage on moulded parts are mould and melt temperature. Autodesk Moldflow Insight software was used for the analysis, while specifications of Nessei NEX 1000 injection moulding machine and P20 mould material were incorporated in this study on top of Acrylonitrile Butadiene Styrene (ABS as a moulded thermoplastic material. Mould temperature, melt temperature, packing pressure and packing time were selected as variable parameters. The results show that the shrinkage have improved 42.48% and 14.41% in parallel and normal directions respectively after the optimisation process.

  1. Thermostructural Analysis of Plate-Type Heat Exchanger Prototypes Considering Weld Properties

    Directory of Open Access Journals (Sweden)

    Kee-nam Song

    2012-01-01

    Full Text Available The mechanical properties in a weld zone are different from those in the parent material owing to their different microstructures and residual weld stresses. Welded plate-type heat exchanger prototypes made of Hastelloy-X alloy were manufactured, and performance tests on the prototypes were performed in a small-scale nitrogen gas loop at the Korea Atomic Energy Research Institute. Owing to a lack of mechanical properties in the weld zone, previous research on the strength analyses of the prototypes was performed using the parent material properties. In this study, based on the mechanical properties of Hastelloy-X alloy obtained using an instrumented indentation technique, strength analyses considering the mechanical properties in the weld zone were performed, and the analysis results were compared with previous research. As a result of the comparison, a thermostructural analysis considering the weld material properties is needed to understand the structural behavior and evaluate the structural integrity of the prototype more reliably.

  2. A SIMPLE FINITE ELEMENT FOR NON-LINEAR ANALYSIS OF COMPOSITE PLATES

    Directory of Open Access Journals (Sweden)

    V.B. Tungikar

    2011-06-01

    Full Text Available Finite Element Analysis for geometrically nonlinear behaviour of laminated composite plates is presented andcompared with the reported investigations. However structural non-linearity is encountered in certain cases andneeds attention. A higher order displacement field that accounts for transverse shear effects under geometricnonlinear condition is employed in the formulation of a four node, rectangular, element with thirteen degrees offreedom per node. First order Zigzag terms have been included in displacement field for improvement in theresponse. Von Karman strain approach is considered in the present analysis. Incremental Pica iterative scheme isused to solve resulting nonlinear equilibrium equations. The formulation demonstrates its excellence in theperformance for predicting response at various lay ups and plies conditions.

  3. Plate reactor as an analysis tool for rapid pyrolysis of biomass

    NARCIS (Netherlands)

    Sepman, A. V.; de Goey, L. P. H.

    2011-01-01

    This work presents a study of the performance of the modified plate reactor by rapid pyrolysis experiments with different biomass samples (MDF, bark pine and Avicel cellulose). The use of the plate instead of a grid allowed us to achieve a more homogeneous temperature distribution across the plate a

  4. Analysis of a Gross Counting Decision Metric for use in Threat Detection During Cargo Container Inspection

    Energy Technology Data Exchange (ETDEWEB)

    Hall, J

    2006-04-28

    LLNL is actively engaged in the development of a variety of advanced technologies for use in detecting potential threats in sea-going cargo containers, particularly the presence of hidden special nuclear materials (SNM). One such project is the so-called ''Nuclear Car Wash'' (NCW), which uses a high-energy neutron probe to scan the container. High-energy, {beta}-delayed {gamma}-rays emitted during the decay of short-lived, neutron-induced fission products are then taken as a signature of fissionable material. There are a number of different threat decision metrics that one could imagine using in conjunction with an inspection system such as the NCW; however, the most straightforward approach might be to simply compare the total number of counts that our detector records during some suitably chosen time interval to the average background signal that one would expect from a ''clean'' container during the same interval. The purpose of this report is to describe the basic statistical properties of a decision metric of this sort and outline the procedures for using it in experimental practice.

  5. Standard test method for determining nodularity and nodule count in ductile iron using image analysis

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2011-01-01

    1.1 This test method is used to determine the percent nodularity and the nodule count per unit area (that is, number of nodules per mm2) using a light microscopical image of graphite in nodular cast iron. Images generated by other devices, such as a scanning electron microscope, are not specifically addressed, but can be utilized if the system is calibrated in both x and y directions. 1.2 Measurement of secondary or temper carbon in other types of cast iron, for example, malleable cast iron or in graphitic tool steels, is not specifically included in this standard because of the different graphite shapes and sizes inherent to such grades 1.3 This standard deals only with the recommended test method and nothing in it should be construed as defining or establishing limits of acceptability or fitness for purpose of the material tested. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address al...

  6. Reference analysis of the signal + background model in counting experiments II. Approximate reference prior

    CERN Document Server

    Casadei, Diego

    2014-01-01

    The objective Bayesian treatment of a model representing two independent Poisson processes, labelled as "signal" and "background" and both contributing additively to the total number of counted events, is considered. It is shown that the reference prior for the parameter of interest (the signal intensity) is well approximated by the widely (ab)used flat prior only when the expected background is very high. For a large portion of the background parameters space, a very simple approximation (the asymptotic form of the reference prior for the limit of perfect prior background knowledge) can be safely used. In all cases, this approximation outperforms the uniform prior. When the asymptotic prior is not good enough, a simple 1-parameter fitting function is often sufficient to obtain an objective Bayesian solution. Otherwise, it is shown that a 2-parameters fitting function is able to reproduce the reference prior in all other cases. The latter is also useful to speed-up the computing time, which can be useful in a...

  7. Vibration Analysis of Rectangular Plates with One or More Guided Edges via Bicubic B-Spline Method

    Directory of Open Access Journals (Sweden)

    W.J. Si

    2005-01-01

    Full Text Available A simple and accurate method is proposed for the vibration analysis of rectangular plates with one or more guided edges, in which bicubic B-spline interpolation in combination with a new type of basis cubic B-spline functions is used to approximate the plate deflection. This type of basis cubic B-spline functions can satisfy simply supported, clamped, free, and guided edge conditions with easy numerical manipulation. The frequency characteristic equation is formulated based on classical thin plate theory by performing Hamilton's principle. The present solutions are verified with the analytical ones. Fast convergence, high accuracy and computational efficiency have been demonstrated from the comparisons. Frequency parameters for 13 cases of rectangular plates with at least one guided edge, which are possible by approximate or numerical methods only, are presented. These results are new in literature.

  8. Analysis on strength and stiffness of double-deck plates filter system of mechanical water treatment plant

    Science.gov (United States)

    Feng, De-zhen; Yu, Qi-qi

    2017-01-01

    Domestic water treatment is a very important technology field. Now, mechanical water treatment technology is getting wide use in production. In the process of life water treatment, filter process is a very important step. In this paper, the strength and deformation of double-deck plates filter system which includes upper filter plate, lower filter plate and reinforced ribs were analyzed with ANSYS and useful results were got. Through the analysis on strength and deformation, the paper found the advantages and disadvantages of production and design of filter systems. After analyzing and comparing the stresses and deformations of several different design schemes, the paper provided the optimized design plan of filter system which can satisfy the strength need and decrease the creep deformation of plastic filter plates.

  9. Analysis of Static Pressure in Area between Back Plate and Cylinder of a Carding Machine with CFD

    Institute of Scientific and Technical Information of China (English)

    HAN Xian-guo; SUN Peng-zi; ZHAO Ye-ping

    2009-01-01

    To analyze static pressure between back plate and cylinder in an A186 carding machine, a fluid model is established. The model takes into account static pressure of airflow near back plate with the numerical simulation method of Computational Fluid Dynamics (CFD) in FLUENT software. The result of the simulation in the model shows that static pressure in this area quickly increases to its maximum then rapidly decreases to a lower fixed value from inlet to outlet along a zone between back plate and cylinder. Both rotating speeds of the cylinder and the taker-in affect static pressure from the inlet to the outlet, of which the cylinder rotating speed has more influence than that of taker-in.. Numerical simulations reveal that static pressure on surface of back plate are in good agreement with the former result of experimental analysis.

  10. Stress protection due to plates: myth or reality? A parametric analysis made using the composite beam theory.

    Science.gov (United States)

    Cordey, J; Perren, S M; Steinemann, S G

    2000-09-01

    A generally accepted idea has been that plate fixation of fractures may result in the structural adaptation of bone (bone loss) to reduced stress (stress protection) with the subsequent danger of refracture after implant removal. This was the negative aspect of stress protection. For this reason, it was proposed that plates made from more deformable materials be used (titanium, polymers or carbon fibres). A theoretical analysis using composite beam theory, with different loading conditions (axial load and bending), demonstrates that stress protection, i.e. early temporary porosis, is a myth. Mechanics of materials shows that when an over-large plate is fixed to small bones (as in small animals, e.g. rabbits), the reduction of bone strain is exaggerated; in contrast, using plates of varying flexibility (steel, titanium or carbon fibre) on large bones leads to strain reduction with an astonishingly similar amplitude.

  11. Numerical analysis on the shoot resistance of heat treated light weight B-grade bulletproof steel plates

    Institute of Scientific and Technical Information of China (English)

    Ma Mingtu; Zhang Jingwen; Zhang Junping

    2014-01-01

    Bulletproof steel plates are widely used for the safety of special vehicles. This paper mainly resear-ches on the shoot resistance of heat treated light weight B-grade bulletproof steel plates through numerical analy-sis. Based on the flow behavior of bulletproof steel plates and bullet at various high strain rates,finite element (FE) model has been set up using ANSYS/LS-DYNA software. The simulation results are compared with the shooting results,which show a good consistency and a high reliability. Therefore,the simulation results are ef-ficient approaches and strategies to decide and select the mechanical property and thickness of bulletproof steel plates,saving a lot of work and the cost of experiments.

  12. Numerical analysis of thermal deformation in laser beam heating of a steel plate

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Chao; Kim, Yong-Rae; Kim, Jae-Woong [Yeungnam University, Kyongsan (Korea, Republic of)

    2017-05-15

    Line heating is a widely used process for plate forming or thermal straightening. Flame heating and induction heating are the traditional heating processes used by industry for line heating. However, these two heating processes are ineffective when used on small steel plates. Thus, the laser beam heating with various power profiles were carried out in this study. A comparison of numerical simulation results and experimental results found a significant difference in the thermal deformation when apply a different power profile of laser beam heating. The one-sinusoid power profile produced largest thermal deformation in this study. The laser beam heating process was simulated by established a combined heat source model, and simulated results were compared with experimental results to confirm the model’s accuracy. The mechanism of thermal deformation was investigated and the effects of model parameters were studied intensively with the finite element method. Thermal deformation was found to have a significant relationship with the amount of central zone plastic deformation. Scientists and engineers could use this study’s verified model to select appropriate parameters in laser beam heating process. Moreover, by using the developed laser beam model, the analysis of welding residual stress or hardness could also be investigated from a power profile point of view.

  13. Two-Dimensional Large Deformation Finite Element Analysis for the Pulling-up of Plate Anchor

    Institute of Scientific and Technical Information of China (English)

    WANG Dong; HU Yu-xia; JIN Xia

    2006-01-01

    Based on mesh regeneration and stress interpolation from an old mesh to a new one, a large deformation finite element model is developed for the study of the behaviour of circular plate anchors subjected to uplift loading. For the determination of the distributions of stress components across a clay foundation, the Recovery by Equilibrium in Patches is extended to plastic analyses. ABAQUS, a commercial finite element package, is customized and linked into our program so as to keep automatic and efficient running of large deformation calculation. The quality of stress interpolation is testified by evaluations of Tresca stress and nodal reaction forces. The complete pulling-up processes of plate anchors buried in homogeneous clay are simulated, and typical pulling force-displacement responses of a deep anchor and a shallow anchor are compared. Different from the results of previous studies, large deformation analysis is of the capability of estimating the breakaway between the anchor bottom and soils. For deep anchors, the variation of mobilized uplift resistance with anchor settlement is composed of three stages, and the initial buried depths of anchors affect the separation embedment slightly. The uplift bearing capacity of deep anchors is usually higher than that of shallow anchors.

  14. Counting Possibilia

    Directory of Open Access Journals (Sweden)

    Alfredo Tomasetta

    2010-06-01

    Full Text Available Timothy Williamson supports the thesis that every possible entity necessarily exists and so he needs to explain how a possible son of Wittgenstein’s, for example, exists in our world:he exists as a merely possible object (MPO, a pure locus of potential. Williamson presents a short argument for the existence of MPOs: how many knives can be made by fitting together two blades and two handles? Four: at the most two are concrete objects, the others being merely possible knives and merely possible objects. This paper defends the idea that one can avoid reference and ontological commitment to MPOs. My proposal is that MPOs can be dispensed with by using the notion of rules of knife-making. I first present a solution according to which we count lists of instructions - selected by the rules - describing physical combinations between components. This account, however, has its own difficulties and I eventually suggest that one can find a way out by admitting possible worlds, entities which are more commonly accepted - at least by philosophers - than MPOs. I maintain that, in answering Williamson’s questions, we count classes of physically possible worlds in which the same instance of a general rule is applied.

  15. Electron-microscopic microprobe analysis on the initial stages of mineral formation in the epiphyseal growth plate.

    Science.gov (United States)

    Barckhaus, R H; Krefting, E R; Althoff, J; Quint, P; Höhling, H J

    1981-01-01

    Dry thin sections (300-500 nm thick) of shock-frozen, freeze-dried and embedded epiphyseal growth plates from the proximal tibia of guinea pigs were cut longitudinally from the plate. Dark round bodies (phi less than 0.5 micron) were observed using the scanning transmission mode of the electron microscope initially directly in the vicinity of the chondrocytes. They gradually spread out in the direction of the metaphysis to the center of the longitudinal septum and represent most probably the matrix vesicles. By use of a microscan of 0.25 X 0.25 micron the element-concentrations of these bodies were measured. The measurements started on those bodies that could be clearly recognized and were extended to a length of 30-40 micron in the metaphyseal direction. To obtain approximate quantitative results the registered CaK alpha and PK alpha x-ray counts were directly compared with counts of fully mineralized regions, the Ca and P contents of which are known. Ca as well as p could be detected in the first visible vesicle-like structures (Ca approximately 0.2%, P approximately 0.4%) and increased steeply in the metaphyseal direction, amounting to approximately 6% Ca and 3% P. These results may lead to the conclusion that Pi becomes split from phosphate esters and transformed into the matrix vesicles already in a very early stage of enrichment. Incorporation of Ca may be coupled with this process.

  16. ANALYSIS ON DYNAMIC CHARACTERISTICS FOR FERROMAGNETIC CONDUCTING PLATES IN A TRANSVERSE UNIFORM MAGNETIC FIELD

    Institute of Scientific and Technical Information of China (English)

    Zheng Xiaojing; Liu Xin'en

    2000-01-01

    In this paper, a set of basic equations for free vibration of ferromagnetic conducting plates in a transverse magnetic field are presented, in which the coupled effects of magnetization and eddy current on the mechanical behavior of the plate are included. Based on the quantitative analyses on the vibration frequency and the values of the critical magnetic field for several supporting conditions of the plate, the effects of the conductivity, the magnetic permeability, the thickness of the plate and supporting conditions on the vibration frequency of the plate and the crifcal magnetic field are discussed.

  17. Error estimates for the analysis of differential expression from RNA-seq count data

    Directory of Open Access Journals (Sweden)

    Conrad J. Burden

    2014-09-01

    Full Text Available Background. A number of algorithms exist for analysing RNA-sequencing data to infer profiles of differential gene expression. Problems inherent in building algorithms around statistical models of over dispersed count data are formidable and frequently lead to non-uniform p-value distributions for null-hypothesis data and to inaccurate estimates of false discovery rates (FDRs. This can lead to an inaccurate measure of significance and loss of power to detect differential expression. Results. We use synthetic and real biological data to assess the ability of several available R packages to accurately estimate FDRs. The packages surveyed are based on statistical models of overdispersed Poisson data and include edgeR, DESeq, DESeq2, PoissonSeq and QuasiSeq. Also tested is an add-on package to edgeR and DESeq which we introduce called Polyfit. Polyfit aims to address the problem of a non-uniform null p-value distribution for two-class datasets by adapting the Storey–Tibshirani procedure. Conclusions. We find the best performing package in the sense that it achieves a low FDR which is accurately estimated over the full range of p-values, albeit with a very slow run time, is the QLSpline implementation of QuasiSeq. This finding holds provided the number of biological replicates in each condition is at least 4. The next best performing packages are edgeR and DESeq2. When the number of biological replicates is sufficiently high, and within a range accessible to multiplexed experimental designs, the Polyfit extension improves the performance DESeq (for approximately 6 or more replicates per condition, making its performance comparable with that of edgeR and DESeq2 in our tests with synthetic data.

  18. Sensitivity analysis of thermal performances of flat plate solar air heaters

    Science.gov (United States)

    Njomo, Donatien; Daguenet, Michel

    2006-10-01

    Sensitivity analysis is a mathematical tool, first developed for optimization methods, which aim is to characterize a system response through the variations of its output parameters following modifications imposed on the input parameters of the system. Such an analysis may quickly become laborious when the thermal model under consideration is complex or the number of input parameters is high. In this paper, we develop a mathematical model to analyse the heat exchanges in four different types of solar air collectors. When building this thermal model we show that for each collector, at quasi-steady state, the energy balance equations of the components of the collector cascade into a single first-order non-linear differential equation that is able to predict the thermal behaviour of the collector. Our heat transfer model clearly demonstrates the existence of an important dimensionless parameter, referred to as the thermal performance factor of the collector, that compares the useful thermal energy which can be extracted from the heater to the overall thermal losses of that collector for a given set of input parameters. A sensitivity analysis of our thermal model has been performed for the most significant input parameters such as the incident solar irradiation, the inlet fluid temperature, the air mass flow rate, the depth of the fluid channel, the number and nature of the transparent covers in order to measure the impact of each of these parameters on our model. An important result which can be drawn from this study is that the heat transfer model developed is robust enough to be used for thermal design studies of most known flat plate solar air heaters, but also of flat plate solar water collectors and linear solar concentrators.

  19. Electrostatic and Small-Signal Analysis of CMUTs With Circular and Square Anisotropic Plates

    DEFF Research Database (Denmark)

    la Cour, Mette Funding; Christiansen, Thomas Lehrmann; Jensen, Jørgen Arendt

    2015-01-01

    Traditionally, Capacitive Micromachined Ultrasonic Transducers (CMUTs) are modeled using the isotropic plate equation and this leads to deviations between analytical calcu- lations and Finite Element Modeling (FEM). In this paper, the deflection is calculated for both circular and square plates...... using the full anisotropic plate equation. It is shown that the anisotropic calculations match perfectly with FEM while an isotropic ap- proach causes up to 10% deviations in deflection. For circular plates an exact solution can be found and for square plates using the Galerkin method and utilizing...... the symmetry of the silicon crystal, a compact and accurate expression for the deflection can be obtained. The deviation from FEM in center deflection is plates is also applied to the CMUT. The deflection of a square plate was measured on fabricated CMUTs using a white light...

  20. A comparative analysis of OTF, NPS, and DQE in energy integrating and photon counting digital x-ray detectors.

    Science.gov (United States)

    Acciavatti, Raymond J; Maidment, Andrew D A

    2010-12-01

    , the difference in NPS and DQE between the two detector types is found to increase with the blurring of the x-ray converter. Ultimately, the expression for the additive white noise of the photon counter is compared against the expression for electronic noise and secondary quantum noise in an energy integrator. Thus, a method is provided to determine the average secondary quanta that the energy integrator must produce for each x ray to have superior DQE to a photon counter with the same PSF. This article develops analytical models of OTF, NPS, and DQE for energy integrating and photon counting digital x-ray detectors. While many subtleties of real imaging systems have not been modeled, this work is illustrative in demonstrating an additive source of white noise in photon counting detectors which has not yet been described in the literature. One benefit of this analysis is a framework for determining the average secondary quanta that an energy integrating detector must produce for each x ray to have superior DQE to competing photon counting technology.

  1. MECHANICAL ANALYSIS OF CYLINDERS BEING UPSET BETWEEN SPHERICAL CONCAVE PLATEN AND CONCAVE SUPPORTING PLATE

    Institute of Scientific and Technical Information of China (English)

    LIU Guohui; XIAO Wenhui; NI Liyong; LIU Zhubai

    2008-01-01

    Mechanical analysis of cylinders being upset between spherical concave platen and concave supporting plate is conducted. Rigid-plastic mechanical models for cylinders are presented. When the ratio of height to diameter, is larger than 1, there exists two-dimensional tensile stress in the deformed body, when the ratio is smaller than 1, there exists shear stress in static hydraulic zone. The former breaks through the theory that there is three-dimensional compressive stress irrespective of any ratio of height to diameter. The latter satisfactorily explains the mechanism of layer-like cracks in disk-shaped forgings and the flanges of forged gear axles. The representation of the two models makes the upsetting theory into correct and perfect stage.

  2. Finite-Element Analysis of Jute- and Coir-Fiber-Reinforced Hybrid Composite Multipanel Plates

    Science.gov (United States)

    Nirbhay, M.; Misra, R. K.; Dixit, A.

    2015-09-01

    Natural-fiber-reinforced polymer composite materials are rapidly gaining interest worldwide both in terms of research and industrial applications. The present work includes the characterization and modeling of jute- and coir-fiber-reinforced hybrid composite materials. The mechanical behavior of a two-panel plate and a sixpanel box structure is analyzed under various loading regimes by using the finite-element software ABAQUS®. Exhaustive parametric studies are also performed to obtain a clear insight into the relationships between various parameters and deflections of the panels and stress distributions in them. Deflections of both the structures are compared and found to be in good agreement with published results. To determine the mechanical behavior of natural-fiber-reinforced composite panels, a finite-element analysis is performed.

  3. Experimental study and analysis on fatigue stiffness of RC beams strengthened with CFRP and steel plate

    Institute of Scientific and Technical Information of China (English)

    LU Yi-yan; HU Ling; LI Shan; WANG Kang-hao

    2016-01-01

    The objective of this work is to investigate the fatigue behavior of reinforced concrete (RC) beams strengthened with externally bonded carbon fiber reinforced polymer (CFRP) and steel plate. An experimental investigation and theoretical analysis were made on the law of deflection development and stiffness degradation, as well as the influence of fatigue load ranges. Test results indicate that the law of three-stage change under fatigue loading is followed by both midspan deflection and permanent deflection, which also have positive correlation with fatigue load amplitude. Fatigue stiffness of composite strengthened beams degrades gradually with the increasing of number of cycles. Based on the experimental results, a theoretical model by effective moment of inertia method is developed for calculating the sectional stiffness of such composite strengthened beams under fatigue loading, and the calculated results are in good agreement with the experimental results.

  4. Using pulse shape analysis to improve the position resolution of a resistive anode microchannel plate detector

    CERN Document Server

    Siwal, Davinder; deSouza, R T

    2015-01-01

    Digital signal processing techniques were employed to investigate the joint use of charge division and risetime analyses for the resistive anode (RA) coupled to a microchannel plate detector (MCP). In contrast to the typical approach of using the relative charge at each corner of the RA, this joint approach results in a significantly improved position resolution. A conventional charge division analysis utilizing analog signal processing provides a position measured resolution of 170 $\\mu$m (FWHM). By using the correlation between risetime and position we were able to obtain a measured resolution of 92 $\\mu$m (FWHM), corresponding to an intrinsic resolution of 64 $\\mu$m (FMHM) for a single Z-stack MCP detector.

  5. Prognostic value of preoperative absolute lymphocyte count in recurrent hepatocellular carcinoma following thermal ablation: a retrospective analysis

    Directory of Open Access Journals (Sweden)

    Li X

    2014-10-01

    Full Text Available Xin Li, Zhiyu Han, Zhigang Cheng, Jie Yu, Xiaoling Yu, Ping Liang Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, People's Republic of China Purpose: To investigate the prognostic value of preoperative absolute lymphocyte count (ALC in recurrent hepatocellular carcinoma (RHCC following thermal ablation. Materials and methods: We retrospectively analyzed the relationship between preoperative ALC and the clinicopathologic factors and long-term prognosis in 423 RHCC patients who underwent curative thermal ablation. Correlation analysis, receiver operating characteristic (ROC calculation, Kaplan–Meier curves, and multivariate regression were used for statistical analysis. Results: The median time to recurrence was 12 months for RHCC patients after thermal ablation. On multivariate Cox regression analysis, preoperative ALC was an independent risk factor for cancer recurrence, along with tumor differentiation and α-fetoprotein level. ALC ≥1.64×109/L defined by ROC calculation was associated with prolonged survival (area under the curve 0.741, P<0.001. Patients with ALC ≥1.64×109/L showed a mean survival of 20.2 months versus 11.6 months for patients with ALC <1.64×109/L (P<0.001. Patients were stratified into high and low groups according to ALC status. After excluding the basic parameters between groups, the 1- and 3-year recurrence rates in the high group were 20.9% and 29.5%, respectively, which were significantly lower than those of the low group (58.4% and 71.9%, respectively; P<0.001. The recurrence-free survival rates in the two groups analyzed by Kaplan–Meier curves were significantly different (P<0.001. Conclusion: Preoperative ALC is a powerful prognostic factor for RHCC recurrence after thermal ablation, which suggests that maintaining a high ALC in RHCC patients might improve cancer outcomes. Keywords: absolute lymphocyte count, recurrent hepatocellular carcinoma, thermal ablation, recurrence  

  6. Second order harmonic guided wave mutual interactions in plate: Vector analysis, numerical simulation, and experimental results

    Science.gov (United States)

    Hasanian, Mostafa; Lissenden, Cliff J.

    2017-08-01

    The extraordinary sensitivity of nonlinear ultrasonic waves to the early stages of material degradation makes them excellent candidates for nondestructive material characterization. However, distinguishing weak material nonlinearity from instrumentation nonlinearity remains problematic for second harmonic generation approaches. A solution to this problem is to mix waves having different frequencies and to let their mutual interaction generate sum and difference harmonics at frequencies far from those of the instrumentation. Mixing of bulk waves and surface waves has been researched for some time, but mixing of guided waves has not yet been investigated in depth. A unique aspect of guided waves is their dispersive nature, which means we need to assure that a wave can propagate at the sum or difference frequency. A wave vector analysis is conducted that enables selection of primary waves traveling in any direction that generate phase matched secondary waves. We have tabulated many sets of primary waves and phase matched sum and difference harmonics. An example wave mode triplet of two counter-propagating collinear shear horizontal waves that interact to generate a symmetric Lamb wave at the sum frequency is simulated using finite element analysis and then laboratory experiments are conducted. The finite element simulation eliminates issues associated with instrumentation nonlinearities and signal-to-noise ratio. A straightforward subtraction method is used in the experiments to identify the material nonlinearity induced mutual interaction and show that the generated Lamb wave propagates on its own and is large enough to measure. Since the Lamb wave has different polarity than the shear horizontal waves the material nonlinearity is clearly identifiable. Thus, the mutual interactions of shear horizontal waves in plates could enable volumetric characterization of material in remote regions from transducers mounted on just one side of the plate.

  7. Anterior cervical discectomy and fusion: analysis of surgical outcome with and without plating.

    Science.gov (United States)

    Mobbs, Ralph J; Rao, Prakash; Chandran, Nadana K

    2007-07-01

    The aim of this study is to analyse the differences in clinical and radiological outcome of anterior cervical discectomy and fusion for cervical degenerative disease, with and without the addition of an anterior cervical locking plate. Although disc arthoplasty is gaining popularity, the anterior cervical decompression and fusion procedure remains the gold standard. The outcome of 242 cases operated between 1991 to 1998 were analysed. Two groups of patients were operated on by the same surgeon. The only difference in technique between the two groups was the addition of an anterior cervical plate, with all other technical details matching, including the use of iliac crest autograft. The indications for surgery for both groups was identical. We made an attempt to study radiological fusion, clinical outcome and complications between the non-plated and plated groups. Our main finding is that the addition of an anterior plate reduces the number of poor clinical outcomes, but does not increase the number of excellent outcomes. Anterior discectomy and fusion with plating in our series had a significantly higher fusion rate; 98% fusion was noted in the plating group as compared to 93.5% in the non-plating group (Fisher's exact test, p=0.029). Union was faster in the plated group with no significant increase in surgical complications. Although clinical outcomes were superior in the plated group for the radiculopathy cohort, excellent outcomes were not significantly higher as compared to the non-plated group. The non-plated group had a significantly higher rate of poor outcomes, with 10% of patients requiring revision surgery for non-union, kyphosis, graft extrusion and graft collapse with foraminal stenosis. 1.8% of the plated group required revision surgery.

  8. Statistical approaches to the analysis of point count data: A little extra information can go a long way

    Science.gov (United States)

    Farnsworth, G.L.; Nichols, J.D.; Sauer, J.R.; Fancy, S.G.; Pollock, K.H.; Shriner, S.A.; Simons, T.R.; Ralph, C. John; Rich, Terrell D.

    2005-01-01

    Point counts are a standard sampling procedure for many bird species, but lingering concerns still exist about the quality of information produced from the method. It is well known that variation in observer ability and environmental conditions can influence the detection probability of birds in point counts, but many biologists have been reluctant to abandon point counts in favor of more intensive approaches to counting. However, over the past few years a variety of statistical and methodological developments have begun to provide practical ways of overcoming some of the problems with point counts. We describe some of these approaches, and show how they can be integrated into standard point count protocols to greatly enhance the quality of the information. Several tools now exist for estimation of detection probability of birds during counts, including distance sampling, double observer methods, time-depletion (removal) methods, and hybrid methods that combine these approaches. Many counts are conducted in habitats that make auditory detection of birds much more likely than visual detection. As a framework for understanding detection probability during such counts, we propose separating two components of the probability a bird is detected during a count into (1) the probability a bird vocalizes during the count and (2) the probability this vocalization is detected by an observer. In addition, we propose that some measure of the area sampled during a count is necessary for valid inferences about bird populations. This can be done by employing fixed-radius counts or more sophisticated distance-sampling models. We recommend any studies employing point counts be designed to estimate detection probability and to include a measure of the area sampled.

  9. BUCKLING ANALYSIS UNDER COMBINED LOADING OF THIN-WALLED PLATE ASSEMBLIES USING BUBBLE FUNCTIONS

    Institute of Scientific and Technical Information of China (English)

    Gao Xuanneng; Zou Yinsheng; Zhou Xuhong

    2000-01-01

    Bubble functions are finite element modes that are zero on the boundary of the element but nonzero at the other point. The present paper adds bubble functions to the ordinary Complex Finite Strip Method(CFSM) to calculate the elastic local buckling stress of plates and plate assemblies. The results indi cate that the use of bubble functions greatly improves the convergence of the Finite Strip Method(FSM) in terms of strip subdivision, and leads to much smaller storage required for the structure stiffness and stability matrices. Numerical examples are given, including plates and plate structures subjected to a combination of longitudinal and transverse compression, bending and shear. This study illustrates the power of bubble func tions in solving stability problems of plates and plate structures.

  10. Analysis of Heat Transfer Behaviour of the Conduction Cold Plate System

    Institute of Scientific and Technical Information of China (English)

    YangChun-xin; DangChao-Bin

    1995-01-01

    The heat-transfer behaviour of the conduction cold plate system used for avionics is investigated in this paper.The steady-state temperature profile for the cold plate is derived and the relationship between the coolant mass flowrate,the heat load and the highest cold plate temperature is established.A model is proposed to describe the transient thermal rosponse of the cold plate under thermal shock condition.The analytic solution of the transient heat transfer within the cold plate is provided.The results of this paper agree with those of the finite element method and can be used for the structural design and performance evaluation of cold plate system.

  11. Flexural-torsional buckling analysis of angle-bar stiffened plates

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, Rahbar Ranji [Amirkabir University of Technology, Tehran (Iran, Islamic Republic of)

    2015-09-15

    The interaction of flexural-torsional buckling modes is critical for stiffened plates with asymmetric stiffeners. However, this interaction is ignored in all design rules because it is complex to characterize. In the literature, the presence of an attached plate is ignored, and stiffened plate is treated as an ordinary asymmetric beam. In the flexural buckling mode, stiffener and the attached plate buckle together; in the torsional buckling mode, the attached plate cannot freely rotate with stiffener. Basic equations of the flexural-torsional buckling modes are deduced based on hybrid beam concept and a new strain distribution assumption for sideway bending of stiffeners. Elastic buckling stresses of different angle-bar stiffened plates are calculated and compared with those generated by the Finite element method (FEM) and those available in the literature. The present method has better agreements with FEM.

  12. Numerical Analysis of a Cold Plate for FM Radio Power Amplifiers

    Science.gov (United States)

    Sabin, M.; Piva, S.

    2014-07-01

    The results of a numerical investigation of heat and fluid flow in a liquid cold plate for FM radio power amplifiers are presented. The objective is to verify, by using a commercial CFD code, the performance of a blister cold plate designed to dissipate the heat generated by a known set of electronic components, in order to limit their maximum temperature during operations. Since in a blister cold-plate mainly the cover is thermally active, the cold-plate is simplified and lightened by using plastics in the base plate. A 3-D conjugate CFD approach, where thermal and fluid flow analyses are combined, is followed. Several design options for the cold plate are examined and the validity of the full 3-D CFD approach in the dimensioning of the cooling systems of electronic equipment is demonstrated.

  13. Sputum neutrophil counts are associated with more severe asthma phenotypes using cluster analysis.

    Science.gov (United States)

    Moore, Wendy C; Hastie, Annette T; Li, Xingnan; Li, Huashi; Busse, William W; Jarjour, Nizar N; Wenzel, Sally E; Peters, Stephen P; Meyers, Deborah A; Bleecker, Eugene R

    2014-06-01

    Clinical cluster analysis from the Severe Asthma Research Program (SARP) identified 5 asthma subphenotypes that represent the severity spectrum of early-onset allergic asthma, late-onset severe asthma, and severe asthma with chronic obstructive pulmonary disease characteristics. Analysis of induced sputum from a subset of SARP subjects showed 4 sputum inflammatory cellular patterns. Subjects with concurrent increases in eosinophil (≥2%) and neutrophil (≥40%) percentages had characteristics of very severe asthma. To better understand interactions between inflammation and clinical subphenotypes, we integrated inflammatory cellular measures and clinical variables in a new cluster analysis. Participants in SARP who underwent sputum induction at 3 clinical sites were included in this analysis (n = 423). Fifteen variables, including clinical characteristics and blood and sputum inflammatory cell assessments, were selected using factor analysis for unsupervised cluster analysis. Four phenotypic clusters were identified. Cluster A (n = 132) and B (n = 127) subjects had mild-to-moderate early-onset allergic asthma with paucigranulocytic or eosinophilic sputum inflammatory cell patterns. In contrast, these inflammatory patterns were present in only 7% of cluster C (n = 117) and D (n = 47) subjects who had moderate-to-severe asthma with frequent health care use despite treatment with high doses of inhaled or oral corticosteroids and, in cluster D, reduced lung function. The majority of these subjects (>83%) had sputum neutrophilia either alone or with concurrent sputum eosinophilia. Baseline lung function and sputum neutrophil percentages were the most important variables determining cluster assignment. This multivariate approach identified 4 asthma subphenotypes representing the severity spectrum from mild-to-moderate allergic asthma with minimal or eosinophil-predominant sputum inflammation to moderate-to-severe asthma with neutrophil-predominant or mixed granulocytic

  14. PERTURBATION ANALYSIS FOR MAGNETO-PLASTIC INSTABILITY OF FERROMAGNETIC BEAM-PLATES WITH GEOMETRIC IMPERFECTION

    Institute of Scientific and Technical Information of China (English)

    ZhouYouhe; GaoYuanwen; ZhengXiaojing

    2004-01-01

    The magneto-plastic instability of a ferromagnetic beam-type plate with simple supports and small initial imperfection is analytically investigated in this paper for that the plastic deformation of the plate with a linear-strain hardening relation is considered when the plate is located in a strong uniformly distributed magnetic field. After the distribution of magnetic fields related to the deflected configuration of plate is imaginably divided into two parts, i.e.,one is related to the flat plate and the other dependent on the perturbation of magnetic fields for which the plate configuration changes from the flat into the deformed state, the perturbation technique is employed to analyze the distribution of the perturbation magnetic fields in and out-of the magnetic medium of the ferromagnetic structure in a transverse magnetic field, which leads to some analytical formulae/solutions for the magnetic fields and the resulting magnetic force exerted on the plate. Based on them, the magneto-plastic buckling and snapping of the plate in a transverse magnetic field is discussed, and the critical magnetic field is analytically formulated in terms of the parameters of geometry and material of the plate employed by solving the governing equation of the magneto-plastic plate in the applied magnetic field. Further, the sensitivity of the initial imperfection on the magneto-plastic instability, expressed by an amplification function, is obtained by solving the dynamic equation of deflection of the plate after the inertial force in the transverse direction is taken into account. The results obtained show that the critical magnetic field is sensitive to the plastic characteristic, e.g., hardening coefficient, and the instability mode and deflection of the plate are dependent on the geometrical imperfection as well.

  15. A reconstructed edge-based smoothed DSG element based on global coordinates for analysis of Reissner-Mindlin plates

    Science.gov (United States)

    Yang, Gang; Hu, De'an; Long, Shuyao

    2017-02-01

    A reconstructed edge-based smoothed triangular element, which is incorporated with the discrete shear gap (DSG) method, is formulated based on the global coordinate for analysis of Reissner-Mindlin plates. A symbolic integration combined with the smoothing technique is implemented to calculate the smoothed finite element matrices, which is integrated along the boundaries of each smoothing cell. Numerical results show that the proposed element is free from shear locking, and its results are in good agreement with the exact solutions, even for very thin plates with extremely distorted elements. The proposed element gives more accurate results than the original DSG element without smoothing, and it can be taken as an alternative element for analysis of Reissner-Mindlin plates. The prominent feature of the present element is that the integration scheme is unified in the smoothed form for all of the finite element matrices.

  16. Stability analysis of the fluttering and autorotation of flow-induced rotation of a hinged flat plate

    Institute of Scientific and Technical Information of China (English)

    MIRZAEISEFAT Sina; FERNANDES Antonio Carlos

    2013-01-01

    This work describes investigations performed on the interaction of uniform current and freely rotating plate about a fixed vertical axis. Fluttering and autorotation are two different motions that may occur during the flow induced rotation. The dimensional analysis proves that the motion in flow induced rotation motion is governed essentially by the dimensionless moment of inertia and Reynolds number. Certain combinations define the stability boundaries between fluttering and autorotation. Fluttering is oscillation of body about a vertical axis and the autorotation is a name given to the case when the body turns continuously about the vertical axis. First, the loads and moment coefficients are calculated by experiments and streamline theory for different angles of attack for a fixed flat plate. Then for dynamic case, a bifurcation diagram is presented based on experiments to classify different motion states of flow induced rotation. Finally, a dynamical model is proposed for stability analysis of flow induced rotation of a flat plate.

  17. SIMULATION AND ANALYSIS OF FLOW PATTERN IN CROSS-CORRUGATED PLATE HEAT EXCHANGERS

    Institute of Scientific and Technical Information of China (English)

    ZHANG Guan-min; TIAN Mao-cheng; ZHOU Shou-jun

    2006-01-01

    Using numerical methodology, the flow fields between two corrugated plates with different values of the corrugation inclination angle β were simulated.The simulation results directly indicate that β affects the flow pattern between corrugated plates, and the results are in good agreement with the experimental results reported by interrelated literature.The results show that the flow pattern between the two plates changes from "double cross-flow" to "zigzag flow" with the increase in β.The reason for the effect on the flow pattern between the two corrugated plates was discussed from the view of the variation of momentum in the direction of corrugation with the variation in β.

  18. Fracture Characteristics Analysis of Double-layer Rock Plates with Both Ends Fixed Condition

    Directory of Open Access Journals (Sweden)

    S. R. Wang

    2014-07-01

    Full Text Available In order to research on the fracture and instability characteristics of double-layer rock plates with both ends fixed, the three-dimension computational model of double-layer rock plates under the concentrated load was built by using PFC3D technique (three-dimension particle flow code, and the mechanical parameters of the numerical model were determined based on the physical model tests. The results showed the instability process of the double-layer rock plates had four mechanical response phases: the elastic deformation stage, the brittle fracture of upper thick plate arching stage, two rock-arch bearing stage and two rock-arch failure stage; moreover, with the rock plate particle radius from small to large change, the maximum vertical force of double rock-arch appeared when the particle size was a certain value. The maximum vertical force showed an upward trend with the increase of the rock plate temperature, and in the case of the same thickness the maximum vertical force increased with the increase of the upper rock plate thickness. When the boundary conditions of double-layer rock plates changed from the hinged support to the fixed support, the maximum horizontal force observably decreased, and the maximum vertical force showed small fluctuations and then tended towards stability with the increase of cohesive strength of double-layer rock plates.

  19. A layer-wise MITC9 finite element for the free-vibration analysis of plates with piezo-patches

    Directory of Open Access Journals (Sweden)

    Maria Cinefra

    2015-04-01

    Full Text Available The present article considers the free-vibration analysis of plate structures with piezoelectric patches by means of a plate finite element with variable through-the-thickness layer-wise kinematic. The refined models used are derived from Carrera’s Unified Formulation (CUF and they permit the vibration modes along the thickness to be accurately described. The finite-element method is employed and the plate element implemented has nine nodes, and the mixed interpolation of tensorial component (MITC method is used to contrast the membrane and shear locking phenomenon. The related governing equations are derived from the principle of virtual displacement, extended to the analysis of electromechanical problems. An isotropic plate with piezoelectric patches is analyzed, with clamped-free boundary conditions and subjected to open- and short-circuit configurations. The results, obtained with different theories, are compared with the higher-order type solutions given in the literature. The conclusion is reached that the plate element based on the CUF is more suitable and efficient compared to the classical models in the study of multilayered structures embedding piezo-patches.

  20. Rainflow counting revisited

    Energy Technology Data Exchange (ETDEWEB)

    Soeker, H. [Deutsches Windenergie-Institut (Germany)

    1996-09-01

    As state of the art method the rainflow counting technique is presently applied everywhere in fatigue analysis. However, the author feels that the potential of the technique is not fully recognized in wind energy industries as it is used, most of the times, as a mere data reduction technique disregarding some of the inherent information of the rainflow counting results. The ideas described in the following aim at exploitation of this information and making it available for use in the design and verification process. (au)

  1. High frequency analysis of a plate carrying a concentrated nonlinear spring-mass system

    Science.gov (United States)

    Culver, Dean; Dowell, Earl

    2016-09-01

    Examining the behavior of dynamical systems with many degrees of freedom undergoing random excitation at high frequency often requires substantial computation. These requirements are even more stringent for nonlinear systems. One approach for describing linear systems, Asymptotic Modal Analysis (AMA), has been extended to nonlinear systems in this paper. A prototypical system, namely a thin plate carrying a concentrated hardening cubic spring-mass, is explored. The study focuses on the response of three principal variables to random, frequency-bounded excitation: the displacement of the mounting location of the discrete spring-mass, the relative displacement of the discrete mass to this mounting location, and the absolute displacement of the discrete mass. The results indicate that extending AMA to nonlinear systems for input frequency bands containing a large number of modes is feasible. Several advantageous properties of nonlinear AMA are found, and an additional reduced frequency-domain modal method, Dominance-Reduced Classical Modal Analysis (DRCMA), is proposed that is intermediate in accuracy and the cost of computation between AMA and Classical Modal Analysis (CMA).

  2. On-plate desalting and SALDI-MS analysis of peptides with hydrophobic silicate nanofilms on a gold substrate.

    Science.gov (United States)

    Duan, Jicheng; Wang, Hui; Cheng, Quan

    2010-11-15

    We report the use of silicate nanofilms for on-plate desalting and subsequently direct laser desorption/ionization-mass spectrometric (LDI-MS) analysis of peptides. A hydrophobic octadecyltrichlorosilane (OTS) monolayer is formed on a calcinated nanofilm on a gold substrate to facilitate sample deposition and interaction with the surface that allows effective removal of MS-incompatible contaminants such as salts and surfactants by simple on-plate washing while the peptides are retained on the spot. By elimination of interferences from matrix-related ions and contaminants, sensitivity of MS analysis has been enhanced over ca. 20 times, leading to improved detection of peptides at the low-femtomolar level. A high recovery rate of the peptides is obtained by using relatively rough nanofilms, which are prepared through a modified layer-by-layer deposition/calcination process. The performance of the films has been investigated with peptide samples in the presence of high salts (NaCl and sodium acetate) and urea. Compared to matrix-assisted laser desorption/ionization analysis with CHCA matrix, LDI with on-plate desalting offers marked improvement for analysis of peptides due to low background ions and reduction of sample complexity. Additionally, selective capture of the hydrophobic components of a protein can be achieved, providing a highly useful strategy for specific peptide enrichment. LDI with on-plate desalting approach has also been successfully applied to peptide analysis from protein digests.

  3. 菌落总数检测纸片法与国标方法的比较研究%A Comparative Study of Petrifilm and GB Traditional Agar Plate in Bacteria Counting

    Institute of Scientific and Technical Information of China (English)

    李宇; 姚卢悦

    2012-01-01

    在利用微生物快速测试纸片和国标法所规定的倾注平板计数法在食品、空气和环节表面环境卫生等几个食品生产企业普遍关注的卫生控制环节进行比较研究和统计分析,力求找出两种测试方法之间的相关性,为全面提升食品企业生产效率,缩短检测流程,以及更好地适应现代化食品品质管理原则提供了新的思路和方法。参照国标GB4789.2—2010和GB15982—1995测试方法,对市场上随机抽取的150份焙烤食品和75个空气及环节表面细菌的采样点,分别用纸片法和国标法进行比对试验。经统计分析,纸片法在食品和空气自然沉降菌检测中与传统的国标方法没有显著差异(p〉0.05),但在环节表面细菌菌落计数检测中与传统的国标方法差异显著(p〈0.05),所得计数结果明显高于传统国标法。%Try to find out the relativity between the new Petrifilm test and the traditional test through total bacteria count tested in food. The air and the surface areas which were very important quality control points in food manufacture. According to the test guideline from the standards GB 4789.2 and GB 15982, compare the total bacteria count difference in food, the air and the surface between the Petrifilm and the traditional methods. Based on the statistic analysis, in food hygiene test and air bacterial sampling test, the result from Petrifilm test was the same with the traditional test shows; in the surface bacterial sampling test, the results showed that the Petrifilm test was much better than the traditional ones.

  4. Comparative analysis of dose rates in bricks determined by neutron activation analysis, alpha counting and X-ray fluorescence analysis for the thermoluminescence fine grain dating method

    Science.gov (United States)

    Bártová, H.; Kučera, J.; Musílek, L.; Trojek, T.

    2014-11-01

    In order to evaluate the age from the equivalent dose and to obtain an optimized and efficient procedure for thermoluminescence (TL) dating, it is necessary to obtain the values of both the internal and the external dose rates from dated samples and from their environment. The measurements described and compared in this paper refer to bricks from historic buildings and a fine-grain dating method. The external doses are therefore negligible, if the samples are taken from a sufficient depth in the wall. However, both the alpha dose rate and the beta and gamma dose rates must be taken into account in the internal dose. The internal dose rate to fine-grain samples is caused by the concentrations of natural radionuclides 238U, 235U, 232Th and members of their decay chains, and by 40K concentrations. Various methods can be used for determining trace concentrations of these natural radionuclides and their contributions to the dose rate. The dose rate fraction from 238U and 232Th can be calculated, e.g., from the alpha count rate, or from the concentrations of 238U and 232Th, measured by neutron activation analysis (NAA). The dose rate fraction from 40K can be calculated from the concentration of potassium measured, e.g., by X-ray fluorescence analysis (XRF) or by NAA. Alpha counting and XRF are relatively simple and are accessible for an ordinary laboratory. NAA can be considered as a more accurate method, but it is more demanding regarding time and costs, since it needs a nuclear reactor as a neutron source. A comparison of these methods allows us to decide whether the time- and cost-saving simpler techniques introduce uncertainty that is still acceptable.

  5. Obliquity along plate boundaries

    Science.gov (United States)

    Philippon, Mélody; Corti, Giacomo

    2016-12-01

    Most of the plate boundaries are activated obliquely with respect to the direction of far field stresses, as roughly only 8% of the plate boundaries total length shows a very low obliquity (ranging from 0 to 10°, sub-orthogonal to the plate displacement). The obliquity along plate boundaries is controlled by (i) lateral rheological variations within the lithosphere and (ii) consistency with the global plate circuit. Indeed, plate tectonics and magmatism drive rheological changes within the lithosphere and consequently influence strain localization. Geodynamical evolution controls large-scale mantle convection and plate formation, consumption, and re-organization, thus triggering plate kinematics variations, and the adjustment and re-orientation of far field stresses. These geological processes may thus result in plate boundaries that are not perpendicular but oblique to the direction of far field stresses. This paper reviews the global patterns of obliquity along plate boundaries. Using GPlate, we provide a statistical analysis of present-day obliquity along plate boundaries. Within this framework, by comparing natural examples and geological models, we discuss deformation patterns and kinematics recorded along oblique plate boundaries.

  6. Finite element analysis of customized reconstruction plates for mandibular continuity defect therapy.

    Science.gov (United States)

    Narra, Nathaniel; Valášek, Jiří; Hannula, Markus; Marcián, Petr; Sándor, George K; Hyttinen, Jari; Wolff, Jan

    2014-01-03

    Large mandibular continuity defects pose a significant challenge in oral maxillofacial surgery. One solution to this problem is to use computer-guided surgical planning and additive manufacturing technology to produce patient-specific reconstruction plates. However, when designing customized plates, it is important to assess potential biomechanical responses that may vary substantially depending on the size and geometry of the defect. The aim of this study was to assess the design of two customized plates using finite element method (FEM). These plates were designed for the reconstruction of the lower left mandibles of two ameloblastoma cases (patient 1/plate 1 and patient 2/plate 2) with large bone resections differing in both geometry and size. Simulations revealed maximum von Mises stresses of 63 MPa and 108 MPa in plates 1 and 2, and 65 MPa and 190 MPa in the fixation screws of patients 1 and 2. The equivalent strain induced in the bone at the screw-bone interface reached maximum values of 2739 micro-strain for patient 1 and 19,575 micro-strain for patient 2. The results demonstrate the influence of design on the stresses induced in the plate and screw bodies. Of particular note, however, are the differences in the induced strains. Unphysiologically high strains in bone adjacent to screws can cause micro-damage leading to bone resorption. This can adversely affect the anchoring capabilities of the screws. Thus, while custom plates offer optimal anatomical fit, attention should be paid to the expected physiological forces on the plates and the induced stresses and strains in the plate-screw-bone assembly.

  7. Counting the Costs of Acquisitions: Using Cost-Benefit Analysis in a Seminary and University Library

    Directory of Open Access Journals (Sweden)

    Verena Getahun

    2009-11-01

    Full Text Available This essay considers how cost-benefit analysis may be used in a small to mid-sized library to identify cost-savings in the acquisitions of monographs. The essay highlights parallel studies conducted at Luther Seminary Library and Bethel University Library which compared prices, discounts, and time costs across a range of vendor types to identify whether searching for the best price per item is cost-effective, and how much this strategy could save yearly in acquisitions. Both libraries found that substantial potential savings were identified through this study.

  8. Make It Count: an Analysis of a Brute-forcing Botnet

    Directory of Open Access Journals (Sweden)

    Veronica Valeros

    2016-02-01

    Full Text Available The smallest element in a botnet is a bot. The behavior of a bot can change dynamically based on the decision of the botmaster. Commonly driven by profit, bots are expected to be profitable. If an infected bot does not fulfill the expectations, the botmaster can instruct the bot to switch it's behavior to serve a better purpose. This paper presents a detailed analysis of a network traffic capture of a machine originally infected by a Gamarue variant. The analysis will uncover the behavior of the bot since the initial infection, inactivity period, delivery of a new payload and the following switch of behavior of the bot. The paper will analyze the infection in detail, including the horizontal brute-forcing activity affecting thousands of WordPress websites. The goal of the paper is to show a concrete example of a bot performing brute-forcing, analyze it, identify the mechanisms used and indicators of compromise that will help detect it.

  9. Trabecular bone characterization on the continuum of plates and rods using in vivo MR imaging and volumetric topological analysis.

    Science.gov (United States)

    Chen, Cheng; Jin, Dakai; Liu, Yinxiao; Wehrli, Felix W; Chang, Gregory; Snyder, Peter J; Regatte, Ravinder R; Saha, Punam K

    2016-09-21

    Osteoporosis is associated with increased risk of fractures, which is clinically defined by low bone mineral density. Increasing evidence suggests that trabecular bone (TB) micro-architecture is an important determinant of bone strength and fracture risk. We present an improved volumetric topological analysis algorithm based on fuzzy skeletonization, results of its application on in vivo MR imaging, and compare its performance with digital topological analysis. The new VTA method eliminates data loss in the binarization step and yields accurate and robust measures of local plate-width for individual trabeculae, which allows classification of TB structures on the continuum between perfect plates and rods. The repeat-scan reproducibility of the method was evaluated on in vivo MRI of distal femur and distal radius, and high intra-class correlation coefficients between 0.93 and 0.97 were observed. The method's ability to detect treatment effects on TB micro-architecture was examined in a 2 years testosterone study on hypogonadal men. It was observed from experimental results that average plate-width and plate-to-rod ratio significantly improved after 6 months and the improvement was found to continue at 12 and 24 months. The bone density of plate-like trabeculae was found to increase by 6.5% (p  =  0.06), 7.2% (p  =  0.07) and 16.2% (p  =  0.003) at 6, 12, 24 months, respectively. While the density of rod-like trabeculae did not change significantly, even at 24 months. A comparative study showed that VTA has enhanced ability to detect treatment effects in TB micro-architecture as compared to conventional method of digital topological analysis for plate/rod characterization in terms of both percent change and effect-size.

  10. Analysis of Crystallographic Textures in Aluminum Plates Processed by Equal Channel Angular Extrusion

    DEFF Research Database (Denmark)

    Li, Saiyi; Mishin, Oleg

    2014-01-01

    A modeling and experimental investigation has been conducted to explore the effect of processing route on texture evolution during equal channel angular extrusion (ECAE) of aluminum plate samples. It is found that although the textures in the plates develop along orientation fibers previously...

  11. Preliminary Analysis for Flow Blockage of Plate Fuel using a Commercial CFD Code

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong-Pil; Park, Suki [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2016-10-15

    A plate-type fuel assembly is widely used in the research reactors in order to enhance power density. This type fuel assembly consists of a number of fuel plates, supporting plates, and narrow rectangular cooling channels between the fuel plates. Due to the narrow rectangular channels consisting of parallel plate fuels and side plates, the cooling channels are isolated from each other so that the cross flow between the channels are completely restricted. When blockage at the inlet of the channel occurs, therefore, the coolant flow through the blocked channel will be greatly reduced or completely interrupted. Accordingly, the blocked channel loses its own cooling capability. This event may cause initiation of nucleate boiling and two phase flow instability (FI) in the blocked channel. In recent years, CFD analyses of flow blockage of a research reactor have been performed by many researchers. The present study reports a 3-dimensional CFD simulation using two-phase model for flow blockage of two channels of a plate-type fuel assembly. The present result shows limited information since the simulation is working at the moment. However, the present study indicates that it is possible to predict a damage propagation of a plate-type fuel assembly due to the flow instability caused by flow blockage using a commercial CFD code.

  12. Finite Element Analysis of the Deformation of Functionally Graded Plates under Thermomechanical Loads

    Directory of Open Access Journals (Sweden)

    A. E. Alshorbagy

    2013-01-01

    Full Text Available The first-order shear deformation plate model, accounting for the exact neutral plane position, is exploited to investigate the uncoupled thermomechanical behavior of functionally graded (FG plates. Functionally graded materials are mainly constructed to operate in high temperature environments. Also, FG plates are used in many applications (such as mechanical, electrical, and magnetic, where an amount of heat may be generated into the FG plate whenever other forms of energy (electrical, magnetic, etc. are converted into thermal energy. Several simulations are performed to study the behavior of FG plates, subjected to thermomechanical loadings, and focus the attention on the effect of the heat source intensity. Most of the previous studies have considered the midplane neutral one, while the actual position of neutral plane for functionally graded plates is shifted and should be firstly determined. A comparative study is performed to illustrate the effect of considering the neutral plane position. The volume fraction of the two constituent materials of the FG plate is varied smoothly and continuously, as a continuous power function of the material position, along the thickness of the plate.

  13. Analysis of transient thermal stress in heat-generating plates and hollow cylinders caused by sudden environmental temperature changes

    Science.gov (United States)

    Rosenberg, G. S.; Schoeberle, D. F.; Valentin, R. A.

    1969-01-01

    Analysis and solution are presented for transient thermal stresses in a free heat-generating flat plate and a free, hollow-generating cylinder as a result of sudden environmental changes. The technique used and graphical results obtained are of interest to the heat transfer industry.

  14. Frequency Response of an Aircraft Wing with Discrete Source Damage Using Equivalent Plate Analysis

    Science.gov (United States)

    Krishnamurthy, T.; Eldred, Lloyd B.

    2007-01-01

    An equivalent plate procedure is developed to provide a computationally efficient means of matching the stiffness and frequencies of flight vehicle wing structures for prescribed loading conditions. Several new approaches are proposed and studied to match the stiffness and first five natural frequencies of the two reference models with and without damage. One approach divides the candidate reference plate into multiple zones in which stiffness and mass can be varied using a variety of materials including aluminum, graphite-epoxy, and foam-core graphite-epoxy sandwiches. Another approach places point masses along the edge of the stiffness-matched plate to tune the natural frequencies. Both approaches are successful at matching the stiffness and natural frequencies of the reference plates and provide useful insight into determination of crucial features in equivalent plate models of aircraft wing structures.

  15. Electric modelling and image analysis of channel flow in bipolar plates

    Energy Technology Data Exchange (ETDEWEB)

    Martin, D.; Gonzalez, L.; Garcia-Alegre, M.C.; Guinea, D. [Instituto de Automatica Industrial, Consejo Superior de Investigaciones Cientificas, 28500 Arganda, Madrid (Spain); Guinea, D.M.; Moreno, B. [Instituto de Ceramica y Vidrio, Consejo Superior de Investigaciones Cientificas, Kelsen 5, 28049 Madrid (Spain)

    2007-07-15

    Bipolar plates are an essential part of Polymer Electrolyte Membrane Fuel Cells (PEMFC) and are related to fluid conduction. The topology of a bipolar plate is critical to the homogeneous distribution of the feeding gases over the accessible zone of the electrode. An electric model that simulates flow in bipolar plates and permits the optimisation of gas feeding in PEMFCs is proposed. As a first approach, an analogy is made between the gas pressure P and an electric voltage U in a circuit and a gas flow F and an electric current I. The fluidic resistance in a bipolar plate channel is thus R=P/F and is equivalent to the electric resistance R=U/I in a branch of a circuit. Computer image processing techniques allow the validation of the present flow estimation approach based on electrical variables. Separate plates were developed to experimentally implement a complete parallel bipolar topology. (author)

  16. Position Measurements with Micro-Channel Plates and Transmission lines using Pico-second Timing and Waveform Analysis

    CERN Document Server

    Adamsa, Bernhard; Bogdan, Mircea; Byrum, Karen; Genat, Jean-Francois C; Grabas, Herve; Frisch, Henry J; Kim, Heejong; Heintz, Mary K; Natoli, Tyler; Northrop, Richard; Oberla, Eric; Meehan, Samuel; May, Edward N; Stanek, Robert; Tang, Fukun; Varner, Gary; Yurtsev, Eugene

    2009-01-01

    The anodes of Micro-Channel Plate devices are coupled to fast transmission lines in order to reduce the number of electronics readout channels, and can provide two-dimension position measurements using two-ends delay timing. Tests with a laser and digital waveform analysis show that resolutions of a few hundreds of microns along the transmission line can be reached taking advantage of a few pico-second timing estimation. This technique is planned to be used in Micro-channel Plate devices integrating the transmission lines as anodes.

  17. Computational Fluid Dynamics Uncertainty Analysis Applied to Heat Transfer over a Flat Plate

    Science.gov (United States)

    Groves, Curtis Edward; Ilie, Marcel; Schallhorn, Paul A.

    2013-01-01

    There have been few discussions on using Computational Fluid Dynamics (CFD) without experimental validation. Pairing experimental data, uncertainty analysis, and analytical predictions provides a comprehensive approach to verification and is the current state of the art. With pressed budgets, collecting experimental data is rare or non-existent. This paper investigates and proposes a method to perform CFD uncertainty analysis only from computational data. The method uses current CFD uncertainty techniques coupled with the Student-T distribution to predict the heat transfer coefficient over a at plate. The inputs to the CFD model are varied from a specified tolerance or bias error and the difference in the results are used to estimate the uncertainty. The variation in each input is ranked from least to greatest to determine the order of importance. The results are compared to heat transfer correlations and conclusions drawn about the feasibility of using CFD without experimental data. The results provide a tactic to analytically estimate the uncertainty in a CFD model when experimental data is unavailable

  18. IR-MALDI-MS analysis of HPTLC-separated phospholipid mixtures directly from the TLC plate.

    Science.gov (United States)

    Rohlfing, Andreas; Müthing, Johannes; Pohlentz, Gottfried; Distler, Ute; Peter-Katalinić, Jasna; Berkenkamp, Stefan; Dreisewerd, Klaus

    2007-08-01

    The application of a recently developed direct coupling of high-performance thin-layer chromatography (HPTLC) and infrared matrix-assisted laser desorption/ionization orthogonal extracting time-of-flight mass spectrometry (Dreisewerd, K.; Müthing, J.; Rohlfing, A.; Meisen, I.; Vukelic, Z.; Peter-Katalinic, J.; Hillenkamp, F.; Berkenkamp, S. Anal. Chem. 2005, 77, 4098-4107) to the analysis of phospholipid mixtures is demonstrated. Mixtures of six phospholipid types were exemplarily analyzed. The sensitivity was found to be in the range between about 10 and 150 pmol of material spotted for HPTLC, depending on phospholipid acidity, Rf value, and ion polarity. The lateral resolution of the analysis is on the order of the laser focus diameter of about 220 x 300 microm2, allowing differentiation between phospholipid species of different acyl chain composition within one single HPTLC band, which were undistiguishable by a mere visual assessment. Analyte diffusion due to the addition of glycerol to the HPTLC plate was found to be-if at all notable-of only minor importance.

  19. Performance Analysis of a Wind Turbine Driven Swash Plate Pump for Large Scale Offshore Applications

    Science.gov (United States)

    Buhagiar, D.; Sant, T.

    2014-12-01

    This paper deals with the performance modelling and analysis of offshore wind turbine-driven hydraulic pumps. The concept consists of an open loop hydraulic system with the rotor main shaft directly coupled to a swash plate pump to supply pressurised sea water. A mathematical model is derived to cater for the steady state behaviour of entire system. A simplified model for the pump is implemented together with different control scheme options for regulating the rotor shaft power. A new control scheme is investigated, based on the combined use of hydraulic pressure and pitch control. Using a steady-state analysis, the study shows how the adoption of alternative control schemes in a the wind turbine-hydraulic pump system may result in higher energy yields than those from a conventional system with an electrical generator and standard pitch control for power regulation. This is in particular the case with the new control scheme investigated in this study that is based on the combined use of pressure and rotor blade pitch control.

  20. Static and Dynamic Structural Response of an Aircraft Wing with Damage Using Equivalent Plate Analysis

    Science.gov (United States)

    Krishnamurthy, T.; Tsai, Frank J.

    2008-01-01

    A process to generate an equivalent plate based on an optimization approach to predict the static and dynamic response of flight vehicle wing structures is proposed. Geometric-scale and frequency-scale factors are defined to construct an equivalent plate with any desired scale to use in simulation and wind tunnel experiments. It is shown that the stiffness and the displacements are scaled linearly with the geometric-scale factor, whereas the load is scaled as the square of the geometric-scale factor. The scaled stiffness of the reference flight vehicle is matched first to construct the equivalent plate. Then the frequency-scale factor is defined to scale the flight vehicle frequencies. The scaled flight vehicle frequencies are matched by placing arbitrary point masses along the equivalent plate geometry. Two simple stiffened-plate examples, one with damage and another without damage, were used to demonstrate the accuracy of the optimization procedure proposed. Geometric-scale factors ranging from 0.2 to 1.0 were used in the analyses. In both examples, the static and dynamic response of the reference stiffened-panel solution is matched accurately. The scaled equivalent plate predicted the first five frequencies of the stiffened panel very accurately. Finally, the proposed equivalent plate procedure was demonstrated in a more realistic typical aircraft wing structure. Two scale equivalent plate models were generated using the geometric-scale factors 1.0 and 0.2. Both equivalent plate models predicted the static response of the wing structure accurately. The equivalent plate models reproduced the first five frequencies of the wing structure accurately.

  1. Computational heat transfer analysis and combined ANN–GA optimization of hollow cylindrical pin fin on a vertical base plate

    Indian Academy of Sciences (India)

    C Balachandar; S Arunkumar; M Venkatesan

    2015-09-01

    In the devices like laptops, microprocessors, the electric circuits generate heat while performing work which necessitates the use of fins. In the present work, the heat transfer characteristics of hollow cylindrical pin fin array on a vertical rectangular base plate is studied using commercial CFD code ANSYS FLUENT© . The hollow cylindrical pin fins are arranged inline. The heat transfer augmentation is studied for different parameters such as inner radius, outer radius, height of the fins and number of pin fins. The base plate is supplied with a constant heat flux in the range of 20–500W. The base plate dimensions are kept constant. The base plate temperature is predicted using Artificial Neural Network (ANN) by training the network based on the results of numerical simulation. The trained ANN is used to analyse the fin in terms of enhanced heat transfer and weight reduction when compared to solid pin fin. Optimization of the hollow cylindrical pin fin parameters to obtain maximum heat transfer from the base plate is carried out using Genetic Algorithm (GA) applied on the trained neural network. The analysis using the numerical simulation and neural network shows that the hollow fins provide an increased heat transfer and a weight reduction of about 90% when compared to solid cylindrical pin fins.

  2. Analysis of flow in a cone-and-plate apparatus with respect to spatial and temporal effects on endothelial cells.

    Science.gov (United States)

    Buschmann, M H; Dieterich, P; Adams, N A; Schnittler, H-J

    2005-03-01

    Endothelial cells, covering the inner surface of vessels and the heart, are permanently exposed to fluid flow, which affects the endothelial structure and the function. The response of endothelial cells to fluid shear stress is frequently investigated in cone-plate systems. For this type of device, we performed an analytical and numerical analysis of the steady, laminar, three-dimensional flow of a Newtonian fluid at low Reynolds numbers. Unsteady oscillating and pulsating flow was studied numerically by taking the geometry of a corresponding experimental setup into account. Our investigation provides detailed information with regard to shear-stress distribution at the plate as well as secondary flow. We show that: (i) there is a region on the plate where shear stress is almost constant and an analytical approach can be applied with high accuracy; (ii) detailed information about the flow in a real cone-plate device can only be obtained by numerical simulations; (iii) the pulsating flow is quasi-stationary; and (iv) there is a time lag on the order of 10(-3) s between cone rotation and shear stress generated on the plate.

  3. Improved non-dimensional dynamic influence function method for vibration analysis of arbitrarily shaped plates with clamped edges

    Directory of Open Access Journals (Sweden)

    Sang-Wook Kang

    2016-03-01

    Full Text Available A new formulation for the non-dimensional dynamic influence function method, which was developed by the authors, is proposed to efficiently extract eigenvalues and mode shapes of clamped plates with arbitrary shapes. Compared with the finite element and boundary element methods, the non-dimensional dynamic influence function method yields highly accurate solutions in eigenvalue analysis problems of plates and membranes including acoustic cavities. However, the non-dimensional dynamic influence function method requires the uneconomic procedure of calculating the singularity of a system matrix in the frequency range of interest for extracting eigenvalues because it produces a non-algebraic eigenvalue problem. This article describes a new approach that reduces the problem of free vibrations of clamped plates to an algebraic eigenvalue problem, the solution of which is straightforward. The validity and efficiency of the proposed method are illustrated through several numerical examples.

  4. 21 CFR 1210.16 - Method of bacterial count.

    Science.gov (United States)

    2010-04-01

    ... FEDERAL IMPORT MILK ACT Inspection and Testing § 1210.16 Method of bacterial count. The bacterial count of milk and cream refers to the number of viable bacteria as determined by the standard plate method of... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Method of bacterial count. 1210.16 Section...

  5. Comparação entre os métodos rápidos SimplateR TPC- CI e PetrifilmR AC e os métodos convencionais de contagem em placas para a enumeração de aeróbios mesófilos em sorvetes Comparasion of SimplateR test and Petrifilm with plate count agar method for detection and enumeration of bacteria in ice cream

    Directory of Open Access Journals (Sweden)

    Anderson de Souza Sant'ana

    2002-01-01

    Full Text Available A contagem de aeróbios mesófilos ou contagem padrão em placas em um produto alimentício reflete a qualidade da matéria-prima, bem como as condições de processamento, manuseio e estocagem. O método de contagem em placas tradicionais requer 48h de incubação para a posterior leitura dos resultados. Comparou-se dois métodos que parecem ser uma boa alternativa para a contagem padrão em placas: SimplateR TPC-CI e PetrifilmR AC. Também avaliou-se a capacidade inibitória do 2, 3, 5 cloreto de trifeniltetrazólio quando adicionado ao Agar Plate Count. Este corante é incolor na forma oxidada e vermelho quando reduzido pelos microrganismos, devido à formação de formazano. O coe-ficiente de correlação obtido, através de estudos conduzidos com 60 amostras de sorvetes, entre 0,866-0,979 indicou uma equivalente sensibilidade dos métodos testados. Somente a contagem padrão em placas com TTC diferiu signicativamente da contagem padrão em placas.The mesophilic aerobic plate count or standard plate count (SPC of fresh food product reflects conditions of raw product quality, processing, handling and storage. Current standard methods require a 48h incubation period to obtain the results. Two rapid alterantive methods: SimplateTM TPC-CI and PetrifilmTM Aerobic Count (AC Plate were compared to standard methods. Also, we evaluated the inhibitory effect of 2, 3, 5 triphenyltetrazolium chloride (TTC when added to Plate Count Agar. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to the formation of formazan. Sixty ice cream samples were used in this study to obtain correlation coefficients, which ranged from 0.866-0.979, indicating equivalent sensitivities to existing methods. Only plate count with TTC differed significantly from the standard plate count.

  6. A Novel Higher-Order Shear and Normal Deformable Plate Theory for the Static, Free Vibration and Buckling Analysis of Functionally Graded Plates

    Directory of Open Access Journals (Sweden)

    Shi-Chao Yi

    2017-01-01

    Full Text Available Closed-form solution of a special higher-order shear and normal deformable plate theory is presented for the static situations, natural frequencies, and buckling responses of simple supported functionally graded materials plates (FGMs. Distinguished from the usual theories, the uniqueness is the differentia of the new plate theory. Each individual FGM plate has special characteristics, such as material properties and length-thickness ratio. These distinctive attributes determine a set of orthogonal polynomials, and then the polynomials can form an exclusive plate theory. Thus, the novel plate theory has two merits: one is the orthogonality, where the majority of the coefficients of the equations derived from Hamilton’s principle are zero; the other is the flexibility, where the order of the plate theory can be arbitrarily set. Numerical examples with different shapes of plates are presented and the achieved results are compared with the reference solutions available in the literature. Several aspects of the model involving relevant parameters, length-to-thickness, stiffness ratios, and so forth affected by static and dynamic situations are elaborate analyzed in detail. As a consequence, the applicability and the effectiveness of the present method for accurately computing deflection, stresses, natural frequencies, and buckling response of various FGM plates are demonstrated.

  7. [Automated measurement of reticulocyte count by flow cytometry. II: Analysis of the blood containing abnormal erythrocytes or giant platelets].

    Science.gov (United States)

    Oyamatsu, T; Shimizu, N; Takeuchi, K; Yamamoto, M; Kawai, Y; Watanabe, K; Iri, H

    1989-07-01

    We have examined the influence of erythrocytes containing inclusion bodies, nucleated red cells or giant platelets on the measurement of reticulocyte count by automated machine, R-1000. Correlation of the reticulocyte count between automated and conventional method was extremely good in the blood containing red cells with Jolly bodies, Pappenheimer bodies or basophilic stippling . However, correlation was poor when the sample contained the nucleated red cells. Reticulocyte count was decreased in the blood with significant amounts of nucleated red cells. Since nucleated red cells themselves are not counted as reticulocytes in the machine, this was considered to be due to increased young reticulocytes which frequently appeared with nucleated red cells. Both cold agglutinated red cells and giant platelets apparently influenced the reticulocyte count by the R-1000. These results suggest that red cells with Jolly bodies, Pappenheimer bodies or basophilic stippling do not influence the automatic counting of reticulocytes. Although nucleated red cells, cold agglutinated red cells and giant platelets affected the reticulocyte count, the machine shows abnormal flags in most of above cases (except highly agglutinated red cells), so that one can recount reticulocytes by conventional method. We conclude the machine can safely count the reticulocytes even in the blood containing abnormal red cells or platelets.

  8. Evaluating formability of LCP plate for sacral fractures with one step inverse forming finite element analysis.

    Science.gov (United States)

    Li, Xiaoda; Zhang, Xiangkui; Hu, Ping; Liu, Weijie; Shen, Guozhe; Zhan, Xianghui

    2015-01-01

    The locking compression plate fixation treatment for the unstable sacral fractures is simple and effective, with less trauma and complications. Some locking compression plate parts have been made of high-strength Plate manufactured by hot stamping process since the demand for lightweight biomedical materials. Finite Element (FE) method of One-Step inverse forming based on deformation theory is the tool to evaluate the formability of locking compression plate panel quickly in initial design for reducing costs and development cycle of Plate. But current one-step inverse forming methods are all suitable for cold stamping, not hot-stamping. This paper proposed one-step inverse forming method and workflow for hot-stamping of locking compression Plate. And the B pillar of a sacral bone was simulated and its computing result was compared with experimental value. The result shows that the proposed method in this paper can quickly evaluate high temperature formability of high-strength Plate. And the method is proposed to be used in initial design.

  9. Analysis and enhancement of flexural wave stop bands in 2D periodic plates

    Energy Technology Data Exchange (ETDEWEB)

    Song, Yubao [Laboratory of Science and Technology on Integrated Logistics Support, National University of Defense Technology, 410073 Changsha (China); The Marcus Wallenberg Laboratory for Sound and Vibration Research, KTH – The Royal Institute of Technology, SE-100 44 Stockholm (Sweden); Feng, Leping [The Marcus Wallenberg Laboratory for Sound and Vibration Research, KTH – The Royal Institute of Technology, SE-100 44 Stockholm (Sweden); Wen, Jihong, E-mail: wenjihong_nudt1@vip.sina.com [Laboratory of Science and Technology on Integrated Logistics Support, National University of Defense Technology, 410073 Changsha (China); Yu, Dianlong; Wen, Xisen [Laboratory of Science and Technology on Integrated Logistics Support, National University of Defense Technology, 410073 Changsha (China)

    2015-07-17

    The band structure and enhancement of flexural wave stop bands in a 2D periodic plate are investigated. A unified method for analysing and designing the stop band of the plates with various attached structures is proposed. The effect of attached structures is considered based on their equivalent parameters (added equivalent mass and equivalent moment of inertia). The influences of the equivalent parameters on the band structures are studied. Three cases are considered: adding pure equivalent mass, pure equivalent moment of inertia and the combination of these two. The stop bands are enhanced via the multi interaction between the host plate and the attached structure. The enhancement pattern is determined, and several ways to obtain a wider combined stop band are presented. The frequency response functions of corresponding finite periodic plates are calculated to verify the stop bands and their enhancement in a number of typical cases. - Highlights: • A unified method for studying the stop band of the plates with various simplified attached structures is proposed. • The enhancement of flexural wave stop bands in a 2D phononic plate is investigated. • The stop bands are widened via multi interaction between the host plate and the attached structure. • The enhancement pattern is determined and several ways to get a wider stop band are presented.

  10. Spatial periodicity of galaxy number counts from Fourier analysis of the large scale surveys of galaxies in the Universe

    CERN Document Server

    Hartnett, John G

    2007-01-01

    A Fourier analysis has been carried out on the galaxy number count as a function of redshift, the $N$-$z$ relation, calculated from redshift data of both the Sloan Digital Sky Survey (SDSS) and the 2dF Galaxy Redshift Survey (2dF GRS). Regardless of the interpretation of those redshifts, the results indicate that galaxies have preferred periodic redshifts. This is the \\textit{picket-fence} structure observed by some. Application of the Hubble law, at low redshift, results in galaxies preferentially located on concentric shells with periodic spacings. This analysis finds significant redshift spacings of $\\Delta z =$ 0.0102, 0.0246, and 0.0448 in the SDSS and strong agreement with the results from 2dF GRS. The combined results from both surveys indicate regular real space spacings of $44.0 \\pm 2.5$ $Mpc$, $102 \\pm 8$ $Mpc$ and $176 \\pm 29$ $Mpc$, for an assumed Hubble's constant $H_0 = 72 km s^{-1} Mpc^{-1}$. These results indicate that it is a real effect and not some observational artifact. The effect is sign...

  11. Three-dimensional analysis of a thick FGM rectangular plate in thermal environment

    Institute of Scientific and Technical Information of China (English)

    陈伟球; 边祖光; 丁皓江

    2003-01-01

    The thermal behavior of a thick transversely isotropic FGM rectangular plate was investigated within the scope of three-dimensional elasticity. Noticing many FGMs may have temperature-dependent properties, the material constants were further considered as functions of temperature. A solution method based on state-space formulations with a laminate approximate model was proposed. For a thin plate, the method was clarified by comparison with the thin plate theory. The influences of material inhomogeneity and temperature-dependent characteristics were finally discussed through numerical examples.

  12. 3D analysis of functionally graded material plates with complex shapes and various holes

    Institute of Scientific and Technical Information of China (English)

    Zhi-yuan CAO; Shou-gao TANG; Guo-hua CHENG

    2009-01-01

    In this paper, the basic formulae for the semi-analytical graded FEM on FGM members are derived. Since FGM parameters vary along three space coordinates, the parameters can be integrated in mechanical equations. Therefore with the parameters of a given FGM plate, problems of FGM plate under various conditions can be solved. The approach uses 1D discretization to obtain 3D solutions, which is proven to be an effective numerical method for the mechanical analyses of FGM structures. Examples of FGM plates with complex shapes and various holes are presented.

  13. Clinical Analysis of Internal Fixation Treatment of Intra-articular Calcaneal Fractures with Titanium Plate.

    Science.gov (United States)

    Chen, Xiao-Dong; Zhang, Chang-Chun; Li, Zhao-Cheng; Zhang, Heng; Zhou, Xin-She; Deng, Min

    2015-05-01

    To explore the clinical effect of internal fixation treatment of intra-articular calcaneal fractures with titanium plate, we used open reduction and internal fixation with titanium plate to 48 treated feet from 42 patients with intra-articular calcaneal fractures. The efficacy of surgical treatment was evaluated based on assessment of pain, function, and line of force aspects according to the American Orthopedic Foot and Ankle Society scoring system. Our data show that internal fixation with titanium plate is an effective treatment for calcaneal fractures. It provides satisfactory reduction, reliable fixation, and early rehabilitation.

  14. Three-dimensional Analysis of Functionally Graded Piezoelectric Plate with Arbitrarily Distributed Material Properties

    Institute of Scientific and Technical Information of China (English)

    LIU Wuxiang; MA Shaokun; WU Hao

    2014-01-01

    An orthotropic functionally graded piezoelectric rectangular plate with arbitrarily distributed material properties was studied, which is simply supported and grounded (electrically) on its four lateral edges. The state equations of the functionally graded piezoelectric material were obtained using the state-space approach, and a Peano-Baker series solution was obtained for the coupled electroelastic fields of the functionally graded piezoelectric plate subjected to mechanical and electric loading on its upper and lower surfaces. The influence of different distributions of material properties on the structural response of the plate was studied using the obtained solutions.

  15. Free Vibration Analysis of Sectorial Plates Using the Triangular Differential Quadrature Method

    Institute of Scientific and Technical Information of China (English)

    李欣; 钟宏志; 何玉红

    2004-01-01

    The triangular differential quadrature method was used to analyze the free vibrations of moderately thick sectorial plates. A triangular serendipity transformation was introduced to map the sectorial domain onto a unit isosceles right triangle. The first six non-dimensional frequencies of the sectorial plates were obtained for various combinations of clamped and simply supported boundary conditions. For sectorial plates with simply supported radial edges, the present results agree well with the available exact solutions and finite element solutions, demonstrating the effectiveness of the method.

  16. Limit State Analysis of Reinforced Concrete Plates subjected to in-plane forces

    DEFF Research Database (Denmark)

    Poulsen, Peter Noe; Damkilde, Lars

    2000-01-01

    A finite element formulation of rigid-plastic plates subjected to in-plane forces is developed using stress-based elements and linear programming. Three elements are established, namely a triangular plate element, a bar element and a beam element. The problem is formulated as a lower bound solution......, and the dual variables are interpreted as displacements. Both load and material optimization are formulated. The method is applied to concrete plate structures modelling both the distributed and the concentrated reinforcement. An efficient computational scheme is used, thereby reducing the size of the problem...

  17. Heat Transfer Analysis of Flat Plate Subjected to Multi-Jet Air Impingement using Principal Component Analysis and Computational Technique

    Directory of Open Access Journals (Sweden)

    Palaniappan Chandramohan

    2017-01-01

    Full Text Available The aim of this work is to investigate experimentally the variation in temperature, heat transfer coefficient and Nusselt number of a hot plate subjected to multi-jet air impingement cooling to use the multi-objective optimization technique to arrive at optimum conditions. A flat plate of 15 cm x 10 cm is heated through a heating foil with a constant heat flux of 7667 W/m2. Air jets with and without swirling action are considered, fixing the distance of target surface from nozzle exit at 2D, 4D and 6D. Reynolds numbers 18000, 20000and 22000 and pipe diameters 8mm, 10mm and 12 mm have been considered for investigation. Experiments are designed and analyzed using Taguchi’s technique, coupled with principal component analysis for multi-variate optimization by calculating multi-response performance index (MRPI. Based on the observations made, it is concluded that lower H/D ratio and higher Reynolds number result in higher heat transfer coefficient, in accordance with the first principles. Heat transfer coefficient obtained for jets with swirl is compared with that of jet without swirling for the same Reynolds number and H/D ratio. Furthermore, it is concluded that introducing swirl results in increase of heat transfer coefficients for all the test conditions for 10mm and 12mm diameter jets. However for 8mm jet, introduction of swirl reduced the heat transfer rate for all the test conditions. From Analysis of Variance (ANOVA, it is found that significant contributions on outputs are due to the effect of H/D ratio and Reynolds number. Confirmation experiments with optimum condition result in improved heat transfer coefficient and Nusselt number. Numerical simulation has also been performed with the optimum condition. It is observed that the simulation results are in consistence with the experimental results.

  18. Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography.

    Science.gov (United States)

    Mölder, A; Sebesta, M; Gustafsson, M; Gisselson, L; Wingren, A Gjörloff; Alm, K

    2008-11-01

    Manual cell counting is time consuming and requires a high degree of skill on behalf of the person performing the count. Here we use a technique that utilizes digital holography, allowing label-free and completely non-invasive cell counting directly in cell culture vessels with adherent viable cells. The images produced can provide both quantitative and qualitative phase information from a single hologram. The recently constructed microscope Holomonitor (Phase Holographic Imaging AB, Lund, Sweden) combines the commonly used phase contrast microscope with digital holography, the latter giving us the possibility of achieving quantitative information on cellular shape, area, confluence and optical thickness. This project aimed at determining the accuracy and repeatability of cell counting measurements using digital holography compared to the conventional manual cell counting method using a haemocytometer. The collected data were also used to determine cell size and cellular optical thickness. The results show that digital holography can be used for non-invasive automatic cell counting as precisely as conventional manual cell counting.

  19. High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection.

    Directory of Open Access Journals (Sweden)

    Priya Choudhry

    Full Text Available Counting cells and colonies is an integral part of high-throughput screens and quantitative cellular assays. Due to its subjective and time-intensive nature, manual counting has hindered the adoption of cellular assays such as tumor spheroid formation in high-throughput screens. The objective of this study was to develop an automated method for quick and reliable counting of cells and colonies from digital images. For this purpose, I developed an ImageJ macro Cell Colony Edge and a CellProfiler Pipeline Cell Colony Counting, and compared them to other open-source digital methods and manual counts. The ImageJ macro Cell Colony Edge is valuable in counting cells and colonies, and measuring their area, volume, morphology, and intensity. In this study, I demonstrate that Cell Colony Edge is superior to other open-source methods, in speed, accuracy and applicability to diverse cellular assays. It can fulfill the need to automate colony/cell counting in high-throughput screens, colony forming assays, and cellular assays.

  20. Prediction of Thermal and Elastic Properties of Honeycomb Sandwich Plate for Analysis of Thermal Deformation

    Energy Technology Data Exchange (ETDEWEB)

    Hong, Seok Min; Lee, Jang Il; Byun, Jae Ki; Choi, Young Don [Korea Univ., Seoul (Korea, Republic of)

    2014-04-15

    Thermal problems that are directly related to the lifetime of an electronic device are becoming increasingly important owing to the miniaturization of electronic devices. To solve thermal problems, it is essential to study thermal stability through thermal diffusion and insulation. A honeycomb sandwich plate has anisotropic thermal conductivity. To analyze the thermal deformation and temperature distribution of a system that employs a honeycomb sandwich plate, the thermal and elastic properties need to be determined. In this study, the thermal and elastic properties of a honeycomb sandwich plate, such as thermal conductivity, coefficient of thermal expansion, elastic modulus, Poisson's ratio, and shear modulus, are predicted. The properties of a honeycomb sandwich plate vary according to the hexagon size, thickness, and material properties.