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Sample records for plate agglutination test

  1. Evaluation of glycerin as preserving agent of chicken serum for plate agglutination test

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    ES de Freitas

    2014-09-01

    Full Text Available Serum is widely used for the purpose of monitoring and diagnosis support for most of poultry diseases. In the case of the serum plate agglutination test (SPA, commonly used to detect antibodies for Salmonella Pullorum (SP, Mycoplasma gallisepticum (MG and Mycoplasma synoviae (MS, serum cannot be frozen because it may result in false positive. Without freezing, serum can last only for a few days. In this experiment, glycerin was evaluated as a serum preservering agent. About 50 samples for each disease and analyzed by SPA test previously were separated. Glycerin was added to serum from commercial chickens, with and without antibodies for SP, MG and MS, in the proportion of 1:1 (serum:glycerin and kept at refrigerated conditions (2 to 8 ºC. For four years they were tested by the SPA, initially weekly, afterward monthly and then annually. The results show that serum with glycerin give consistent and valid results according to the kind of antibodies present for the period tested. Sera that glycerin was not added to, the results were valid only for the first week. From the second week on, microbial growth affected the test results of the sera without glycerin. Our investigation shows that glycerin can be used to preserve chicken serum for SPA under refrigerated conditions. It is an easy, simple and cheap procedure that can extend serum shelf life, useful mainly for control sera.

  2. Simple solutions to false results with plate/slide agglutination tests in diagnosis of infectious diseases of man and animals.

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    Saxena, Hari Mohan; Chothe, Shubhada; Kaur, Paviter

    2015-01-01

    We have developed a new Superagglutination test for serodiagnosis of infectious diseases. It differs from conventional plate/slide agglutination tests (PAT/SAT) by three additional steps: prior staining of serum antibody by adding a dye and addition of diluted biotinylated antiglobulin and avidin in sequence after mixing the antigen with the test serum. The new steps circumvent the problems of false positive and false negative results of PAT/SAT. In serodiagnosis of brucellosis, Superagglutination test had higher positive predictive value and specificity than Rose Bengal Plate Test (RBPT) and Standard Tube Agglutination Test (STAT) and higher negative predictive value and sensitivity than RBPT, STAT, ELISA and Complement Fixation Test (CFT).•Superagglutination is a simple, accurate and economic screening test for infections.•More specificity, sensitivity, positive & negative predictive value than RBPT, STAT.•More sensitivity, negative predictive value than ELISA and Complement Fixation Test.

  3. Comparative evaluation of Rose Bengal plate agglutination test, mallein test, and some conventional serological tests for diagnosis of equine glanders.

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    Naureen, Abeera; Saqib, Muhammad; Muhammad, Ghulan; Hussain, Muhammad H; Asi, Muhammad N

    2007-07-01

    The Rose Bengal plate agglutination test (RBT) was evaluated for the diagnosis of equine glanders, and its diagnostic efficiency was compared with that of mallein and other serological tests, including indirect hemagglutination test (IHAT), complement fixation test (CFT), and modified counter immunoelectrophoresis test (mCIET). Sera from 70 naturally infected culture-positive, 96 potentially exposed cohorts, and 110 healthy equines were tested. All tests but mCIET showed 100% specificity when testing the sera from glanders-negative equines. The calculated sensitivities of RBT, IHAT, CFT, mCIET, and mallein test when testing culture-positive equines were 90.0, 97.1, 91.4, 81.4, and 75.7%, respectively. The RBT was significantly (P glandered and nonglandered animals, the highest agreement (0.987) was found between RBT and CFT followed by RBT and IHAT (0.940), RBT and mallein test (0.871), and RBT and mCIET (0.852). Because the RBT is simpler and rapid to perform, the inclusion of the test as a supplementary test for the diagnosis of glanders in field conditions is recommended.

  4. Seroprevalence of Mycoplasma gallisepticum antibody by ELISA and serum plate agglutination test of laying chicken

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    Md. Zulfekar Ali

    2015-01-01

    Full Text Available Aim: Mycoplasma gallisepticum (MG is important avian pathogen responsible for chronic respiratory disease of chicken and turkeys, which result in large economic loss for the poultry industry. The objectives of this study were determination of seroprevalence of MG antibody of commercial layer chicken at laying period in selected areas of Bangladesh. Materials and Methods: A total of 563 blood samples were collected randomly from selected commercial layer chickens at laying period during the period from July to December, 2013. Indirect enzyme linked immunosorbent assay (iELISA and serum plate agglutination (SPA test were performed to detect the presence of antibodies against MG. Results: Of 563 samples, 64.47% and 56.13% showed an overall prevalence of MG antibodies in iELISA and SPA test respectively. Prevalence of MG was recorded the highest (69.63% at 50-55 weeks of age compared with lowest (53.26% at 56-61 weeks of age (p<0.05. Significant (p<0.05 effect of breed were observed in the seroprevalence of MG infection in layer birds in the present study. The overall, 68.77%, 63.74% and 59.37% prevalence were found respectively in sonali, ISA Brown and White leg horn. The prevalence of MG antibodies was the highest (70.13% in December followed by November (68%, October (65.67%, August (63.46%, September (58.54% and July (51.78% month. The seroprevalence of MG antibodies was higher (69.63% in most of the large flocks and lower (56.82% in small flocks. Conclusion: Therefore, might be suggested that the commercial layer farms should be routinely checked to monitor MG infection and the reactor birds should be culled since MG organism has the potential to transmit vertically. The correlation between MG antibody in month and flock size was not significant (p=0.359 and p=0.868, respectively.

  5. Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes

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    S. N. Ghodasara

    2010-04-01

    Full Text Available A total of 180 serum samples (107 cows, 73 buffaloes from cases of abortion and various reproductive disorders were collected for detection of Brucella antibody by Rose Bengal Plate Agglutination Test (RBPT, Serum Tube Agglutination Test (STAT and indirect- ELISA (i-ELISA. The overall prevalence of brucellosis by RBPT, STAT and i-ELISA were 11.21%, 16.00% and 24.30% in cows 9.59%, 12.33% and 26.03% in buffaloes respectively. Overall seroprevalence of Brucellosis in cases of abortion, R.O.P. by RBPT, STAT and i-ELISA were 11.32%, 16.04% and 32.08% respectively. When three serological tests were compared, seropositivity was found highest by i-ELISA (25%, followed by STAT (14.45% and RBPT (10.56%. The results shows higher prevalence of brucellosis in cases of abortion and R.O.P., while at lower level from various reproductive disorders as detected serologically indicating endemicity of the infection in villages around Anand city, Gujarat. [Vet. World 2010; 3(2.000: 61-64

  6. COMPARATIVE STUDY ON EFFICACY OF ROSE BENGAL PLATE TEST (I{BPT AND SERUM AGGLUTINATION TEST (SAT FOR DETECTING THE INCIDENE OF BRUCELLOSIS IN BUFFALOES (Bubolus bubolis

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    Idrees Ali Zahid, Ishtiaq Ahmad and Umer Hayat

    2002-03-01

    Full Text Available Rose Bengal Plate Test (RBPT and Serum Agglutination Test (SAT were applied for the diagnosis of brucellosis in 240 buffaloes maintained at organized livestock farms in Punjab, to measure their comparative efficacy. Based on RBPT and SAT, 11.25 (n=27 and 10.42 percent (n=25 buffaloes were found seropositive, 11.67 (n 28 and 4.58 percent (n= 11 animals showed doubtful results, while 77.08 (n= 185 and 85 percept (n= 204 animals were found seronegative, respectively. Rose Bengal Plate Test detected higher percentages of seropositive, doubtful and seronegative cases than those detected by Serum Agglutination Test, which showed lower percentages or seropositive, doubtful and seronegative cases. This study indicated that SAT is more sensitive and reliable diagnostic test for the detection of Brucella aborlus, antibodies in buffaloes.

  7. A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay

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    Anju Mohan

    2016-07-01

    Full Text Available Aim: We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with strain 19. Materials and Methods: The antibody titers were measured by standard tube agglutination test (STAT, microtiter plate agglutination test (MAT, indirect hemagglutination assay (IHA, and indirect enzyme-linked immunosorbent assay (iELISA as per standard protocols. Results: The mean STAT titers were 1.963±0.345 in infected cattle and 1.200±0.155 in healthy vaccinated cattle. The difference was extremely significant (p<0.0001. The mean MAT titers were 2.244±0.727 in infected cattle and 1.200±0.155 in healthy vaccinated cattle. The difference was very significant (p<0.005. The mean IHA titers in infected cattle were 2.284±0.574, and those in healthy vaccinated cattle were 1.200±0.155. The difference was extremely significant (p=0.0002. However, the difference in mean iELISA titers of infected cattle (1.3678±0.014 and healthy vaccinated cattle (1.367±0.014 was non-significant. The infected animals showed very high titers of agglutinating antibodies compared to the vaccinated animals. However, it cannot be ascertained whether these antibodies are due to vaccine or response to infection. Since the infected animals had been vaccinated earlier, the current infection may suggest that vaccination was unable to induce protective levels of antibody. The heightened antibody response after infection may also indicate a secondary immune response to the antigens common to the vaccine strain and wild Brucella organisms. Conclusion: The brucellosis infected animals showed very high titers of agglutinating antibodies compared to the vaccinated animals.

  8. [Latex agglutination test in amebic liver abscess].

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    Gómez Maganda y Silva, T; García Carrizosa, R; Torres Valadez, F; Ortiz Ramírez, E; Villaseñor de la Parra, C; Flores González, A; Gómez García, E

    1978-01-01

    Amebic hepatic abscesses are one of the most frequent and serious complications of intestinal amibiasis. Although many methods exists with which the diagnosis can be made, frequently problems do arise. Serologic reactions play an important role in the diagnosis of amebic hepatic abscess. Among the most useful of the serological tests, is that which evaluates agglutination with latex particles. Latex agglutination was positive in 98.5% of 200 cases of proved amebic hepatic abscess. The pros and cons of the utility of this test compared with other serological tests are discussed. It is concluded that or the especialist as well as the general practicioner latex agglutination can be extremely useful in the diagnosis of amebic hepatic abscess.

  9. 虎红平板凝集试验确诊布鲁杆菌病截断值的探讨%Cut-off value of rose bengal plate agglutination test in rapid diagnosis of brucellosis

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    马志东; 刘云霞; 李艳红; 李建云

    2013-01-01

    Objective To find out the cut-off value of rose bengal plate agglutination test(RBPT) in rapid diagnosis of brucellosis.Methods From May to June 2009,398 people who came to the outpatient department of Inner Mongolia Center for Endemic Disease Control and Research were diagnosed brucellosis by RBPT and tube agglutination test (SAT).Tube agglutination test as a gold standard,rose bengal plate agglutination test,its authenticity and reliability were evaluated.Results Taking positive predictive value 100.0% as the selection standard,the cut-off value of RBPT was "++" which could be used to diagnose brucellosis.The sensitivity was 83.3%; the specificity was 100.0%; the Youden index was 0.832; and the compliance rote was 89.9%.Conclusion The cut-off value "++" of RBPT to diagnosis brucellosis is worthy of clinical promotion.%目的 探讨虎红平板凝集试验确诊布鲁杆菌病凝集度的截断值.方法 内蒙古地方病防治研究中心门诊部选择2009年5月至2009年6月,进行布鲁杆菌病检查的398人,进行试管凝集试验和虎红平板凝集试验检测,以试管凝集试验为金标准,探讨虎红平板凝集试验诊断布鲁杆菌病的截断值,并对可靠性和真实性进行评价.结果 以阳性预测值100.0%作为筛选标准,虎红平板凝集试验快速确诊布鲁杆菌病的截断值为“++”,以该凝集度强度作为截断值进行布鲁杆菌病诊断,灵敏度为83.3%,特异度为100.0%,约登指数为0.823,符合率为89.9%.结论 以虎红平板凝集试验的“++”作为截断值诊断布鲁杆菌病,值得临床推广.

  10. Disseminated cryptococcal lymphadenitis with negative latex agglutination test

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    XU Xiao-guang; BI Xin-ling; WU Jian-hua; XU Hong; LIAO Wan-qing

    2012-01-01

    We reported an unusual case of disseminated cryptococcal lymphadenitis in an immunocompetent host who presented with fever and lymphadenopathy,which were the only two symptoms and signs.Latex agglutination test of serum and cerebrospinal fluid (CSF) were negative,while lymph node biopsy showed Cryptococcus neoformans.A diagnosis of disseminated cryptococcal lymphadenitis was made.Then the patient was treated with amphotericin B for 15 days as initial therapy and itraconazole for 6 months as maintenance therapy respectively.The patient received re-examination per 6 months and was followed up for 2 years.Swollen lymph nodes diminished gradually,and no fever or other symptoms were found.Latex agglutination test of serum and CSF were negative throughout the follow-up period,and anti-HIV,syphilis and tuberculosis antibody were all negative.

  11. Interpretation of microscopic agglutination test for leptospirosis diagnosis and seroprevalence

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    Chintana Chirathaworn; Rajada Inwattana; Yong Poovorawan; Duangjai Suwancharoen

    2014-01-01

    Determination of antibody titer by microscopic agglutination test (MAT) has been used as a tool for leptospirosis diagnosis. Four fold or greater rise in antibody titers between acute and convalescent sera suggests recent Leptospira infection. In addition, results obtained by MAT have been used to predict infecting serovars. However, cross reactivity among various Leptospira serovars have been reported when patient sera were tested with a battery of Leptospira serovars. This study demonstrates cross- reactivity among several Leptospira serovars when MAT was performed on leptospirosis sera. The data support a role of MAT as a tool for diagnosis. However, for information on infecting serovars, Leptospira isolation and molecular identification should be performed.

  12. Latex agglutination test (LAT) for the diagnosis of typhoid fever.

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    Sahni, Gopal Shankar

    2013-06-01

    The efficacy of latex agglutination test in the rapid diagnosis of typhoid fever was studied and the result compared with that of blood culture. This study included 80 children suffering from typhoid fever, among which 40 were confirmed by blood culture isolation and 40 had possible typhoid fever based on high Widal's titre (a four-fold rise in the titre of antibody to typhi "O" and "H" antigen was considered as a positive Widal's test result). Eighty children, 40 with febrile illness confirmed to be other than typhoid and 40 normal healthy children were used as negative controls. The various groups were: (i) Study group ie, group I had 40 children confirmed by culture isolation of Salmonella typhi(confirmed typhoid cases). (ii) Control groups ie, (a) group II with 40 febrile controls selected from paediatrics ward where cause other than S typhi has been established, (b) group III with 40 afebrile healthy controls that were siblings of the children admitted in paediatric ward for any reason with no history of fever and TAB vaccination in the last one year, and (c) group IV with 40 children with high Widal's titre in paired sera sample. Widal's test with paired sera with a one week interval between collections were done in all 40 patients. Latex aggtutination test which could detect 900 ng/ml of antigen as observed in checker board titration, was positive in all 40 children from group I who had positive blood culture and in 30 children from group IV who had culture negative and had high Widal's titre positive. Latex agglutination test was positive in 4 children in group II and none in group III. Using blood culture positive cases as true positive and children in groups II and III as true negative, the test had a sensitivity of 100% and specificity of 96%. Latex agglutination test was found to be significantly sensitive (100%) and specific (96%) and could detect 75% more cases in group IV (possible typhoid cases). Thus latex agglutination test can be used for rapid

  13. Latex agglutination test for field diagnosis of haemorrhagic septicaemia

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    Lily Natalia

    2002-10-01

    Full Text Available Pasteurella multocida is bacterial pathogens that cause haemorrhagic septicaemia (HS in cattle and buffaloes. Various tests have been used to differentiate types of P. multocida, as well as to diagnose this specific disease. A latex agglutination test has been developed for the detection of P. multocida B:2 which is the causal agent of HS. This test is a rapid and simple test suitable for local laboratorium to diagnose HS cases in the field. A heat stable antigen consisting of mainly lipopolysaccharide (LPS extract of formalin killed P. multocida 0019 was used to produce specific antibody against P. multocida B:2. The antiboy was then used to sensitise latex particles. Latex agglutination test have been used to screen some P. multocida field isolates and this test have been proven to be specific, simple and easy to be used in detecting P. multocida B:2. The specificity is due to antibodies recognising LPS or LPS protein complexes. Sensitised latex was stable at 4° C for at least12 months. This test should be used as an aid to diagnosis and employed principally to confirm and support clinical and post mortem findings of HS.

  14. Interpretation of microscopic agglutination test for leptospirosis diagnosis and seroprevalence

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    Chintana Chirathaworn

    2014-05-01

    Full Text Available Determination of antibody titer by microscopic agglutination test (MAT has been used as a tool for leptospirosis diagnosis. Four fold or greater rise in antibody titers between acute and convalescent sera suggests recent Leptospira infection. In addition, results obtained by MAT have been used to predict infecting serovars. However, cross reactivity among various Leptospira serovars have been reported when patient sera were tested with a battery of Leptospira serovars. This study demonstrates cross-reactivity among several Leptospira serovars when MAT was performed on leptospirosis sera. The data support a role of MAT as a tool for diagnosis. However, for information on infecting serovars, Leptospira isolation and molecular identification should be performed.

  15. [Evaluation of latex agglutination test for anti-treponemal antibody in comparison with chemical luminescence tests].

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    Watanabe, Naomi; Nagatomo, Ritsuko; Okubo, Shigeo; Yokota, Hiromitsu; Ikeda, Hitoshi; Yatomi, Yutaka

    2011-02-01

    The performance of a latex agglutination test (Mediace TPLA) in the detection of anti-treponemal antibody was evaluated in comparison with chemical luminescence tests (LumipulsII-N and Architect TPAb) in 346 cases. Anti-treponemal antibody was further determined by immunochromatography and immunoblotting tests and additionally evaluated by a serological test for syphilis with lipoidal antigens. The total concordance rate between the latex agglutination test and chemical luminescence tests ranged from 96% to 97%: the positive concordance rate ranged from 96% to 97%, and the negative concordance rate, from 97% to 98%. The latex agglutination test showed two false positive cases, and each chemical luminescence test showed two false positive cases, respectively. In eight cases, only the latex agglutination test showed negative results; all specimens contained anti-treponemal antibodies. However, none of these was considered to be a false positive and each was treated as syphilis based on the results of confirmatory analysis with immunochromatography and immunoblotting tests and a serological test for syphilis. The discordant results in the latex agglutination test and chemical luminescence tests may be caused by the different antigenisity of each test. With detailed analysis of those sera treated as syphilis, each specimen was found to contain various antibodies against syphilitic antigens, suggesting that there was a different specificity of native and recombinant antigens. Based on the present results for the comparison between the latex agglutination test and chemical luminescence tests, it was considered that further investigation is necessary to clarify the anti-treponemal antibody profile of syphilis at the disease stage.

  16. Latex agglutination: diagnose the early cryptococcus neoformans test of capsular polysaccharide antigen.

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    Wang, Huanrong; Yuan, Xueqian; Zhang, Lifeng

    2015-01-01

    This paper aims to discuss the early diagnosis value of latex agglutination test in Cryptococcal meningitis. The cerebrospinal fluid (CSF) of 112 patients with definite Cryptococcal meningitis and 26 patients with tubercular meningitis and virus meningitis were collected, latex agglutination test is adopted to detect Cryptococcal capsular polysaccharide antigen. Then it was compared with fungal culture and direct microscopy method for evaluating the sensitivity and specificity of the diagnosis. The sensitivity of three methods including latex agglutination test, fungal culture and direct microscopy was 91.1%,69.6% and 73.2% respectively. The specificity of latex agglutination test was 96.0%, 100% and 100% respectively. That latex agglutination test to detect Cryptococcal capsular polysaccharide antigen could be taken as the early diagnostic method of Cryptococcus neoformans meningitis.

  17. Problems with rapid agglutination methods for identification of Staphylococcus aureus when Staphylococcus saprophyticus is being tested.

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    Gregson, D B; Low, D E; Skulnick, M; Simor, A E

    1988-01-01

    Six rapid agglutination tests for identification of Staphylococcus aureus were evaluated by using 62 strains of S. aureus, 63 strains of S. saprophyticus, and 67 strains of other coagulase-negative staphylococci. S. saprophyticus was responsible for 19 of 26 false-positive results and 20 uninterpretable reactions. Thus, urinary staphylococcal isolates that are positive by rapid agglutination tests may require other confirmatory tests for the identification of possible S. saprophyticus. PMID:3410950

  18. Problems with rapid agglutination methods for identification of Staphylococcus aureus when Staphylococcus saprophyticus is being tested.

    OpenAIRE

    Gregson, D B; Low, D E; Skulnick, M; Simor, A. E.

    1988-01-01

    Six rapid agglutination tests for identification of Staphylococcus aureus were evaluated by using 62 strains of S. aureus, 63 strains of S. saprophyticus, and 67 strains of other coagulase-negative staphylococci. S. saprophyticus was responsible for 19 of 26 false-positive results and 20 uninterpretable reactions. Thus, urinary staphylococcal isolates that are positive by rapid agglutination tests may require other confirmatory tests for the identification of possible S. saprophyticus.

  19. Nuclease enhancement of specific cell agglutination in a serodiagnostic test for Neisseria gonorrhoeae.

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    Arko, R J; Wong, K H; Peacock, W L

    1979-01-01

    Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. Cultures of Corynebacterium vaginalis, N. meningitidis, N. catarrhalis, N. sicca, and N. lactamicus were also tested in the slide agglutination procedure. The addition of both deoxyribonuclease and ribonuclease (1 mg/ml) to the cell suspension medium of phosphate-buffered saline improved the sensitivity and specificity of the agglutination reaction for N. gonorrhoeae. Problems relating to the agglutination test as an aid in identification of N. gonorrhoeae are discussed. PMID:110830

  20. A Microtitration Agglutination Test for Detecting Group E Streptococcus Infection in Swine

    OpenAIRE

    1982-01-01

    A microtitration agglutination test was developed and evaluated for detecting infection of swine with group E streptococci type IV, the most common causative agent of streptococcic lymphadenitis of swine.

  1. Evaluation of a C-reactive protein latex agglutination detection test with sera from patients with sexually transmitted diseases.

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    Schalla, W O; Arko, R J; Thompson, S E

    1984-01-01

    A total of 149 sera, including 79 pre- and posttreatment sera from 33 patients with disseminated gonococcal infections, 18 from patients with uncomplicated gonococcal infections, 6 from patients with pelvic inflammatory disease, 4 from patients with genital Chlamydia trachomatis infections, and 42 from normal volunteers, were examined for C-reactive protein with a latex agglutination C-reactive protein detection kit (Difco Laboratories, Detroit, Mich.). Results were quantitated with LC-Partigen C-reactive protein radial immuno-diffusion plates (Calbiochem-Behring, La Jolla, Calif.). Positive latex agglutination results were observed in all of the pretreatment sera and some of the posttreatment sera of patients with disseminated gonococcal infections and in two sera from patients with pelvic inflammatory disease, which corresponded to quantitative C-reactive protein levels in the radial immunodiffusion plates. C-reactive protein levels were not detectable in the serum samples from normal volunteers or patients with uncomplicated gonococcal infections or genital chlamydial infections. Positive latex agglutination occurred as early as 20 s in sera with high C-reactive protein levels, and all positive results were observed within 90 s of the 3-min test limit. Positive latex test results were obtained with C-reactive protein levels as low as 1 mg/dl (10 micrograms/ml). PMID:6440907

  2. Simultaneous detection of pathogenic bacteria using agglutination test based on colored silica nanoparticles.

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    Yu, Hui; Zhao, Guangying; Dou, Wenchao

    2015-01-01

    Aimed to explore an agglutination test which can simultaneously detect two pathogenic bacteria, an agglutination test based on colored silica nanoparticles (colored-SiNps) was established in this work. Monodisperse colored-SiNps were used as agglutination test carriers; red-SiNps and blue-SiNps were prepared by reverse microemulsion with C.I. Reactive red 136 and C.I. Reactive Blue 14. Then the red-SiNps were sensitized with antibodies against E. sakazaki and denoted as IgG-red-SiNps; The blue-SiNps were coated with antibodies against S. pullorum and S. Gallinarum and denoted as IgGblue- SiNps. The mixture solution of IgG-red-SiNps and IgG-blue-SiNps could simultaneously agglutinate with E. sakazakii and S. pullorum and S. gallinarum on glass slide. The E. sakazakii and S. pullorum and S. gallinarum could be simultaneously detected by agglutination test with obvious agglutination phenomena. The E. sakazakii and S. pullorum and S. gallinarum could both be detected in a range from 4×10(3) to 4×10(9) CFU/mL. The pullorum and S. gallinarum and E. sakazakii in the infected food sample were detected by mixture solution of IgG-red-SiNps and IgG-blue-SiNps too. This agglutination test was easy and rapid, it might be useful for in situ rapid detection method for simultaneously screening different pathogenic microorganisms of foods and feeds in the field.

  3. Automated point-of-care testing for ABO agglutination test: proof of concept and validation.

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    El Kenz, H; Corazza, F

    2015-07-01

    ABO-incompatible red blood cell transfusions still represent an important hazard in transfusion medicine. Therefore, some countries have introduced a systematic bedside ABO agglutination test checking that the right blood is given to the right patient. However, this strategy requires an extremely time-consuming learning programme and relies on a subjective interpretation of ABO test cards agglutination. We developed a prototype of a fully automated device performing the bedside agglutination test that could be completed by reading of a barcoded wristband. This POCT checks the ABO compatibility between the patient and the blood bag. Proof of concept and analytical validation of the prototype has been completed on 451 blood samples: 238 donor packed red blood cells, 137 consecutive unselected patients for whom a blood group determination had been ordered and on 76 patient samples selected with pathology that could possibly interfere with or impair performances of the assay. We observed 100% concordance for ABO blood groups between the POCT and the laboratory instrument. These preliminary results demonstrate the feasibility of ABO determination with a simple POCT device eliminating manipulation and subjective interpretation responsible for transfusion errors. This device should be linked to the blood bank system allowing all cross-check of the results. © 2015 International Society of Blood Transfusion.

  4. Microplate Agglutination Test for Canine Brucellosis Using Recombinant Antigen-Coated Beads.

    Science.gov (United States)

    Castillo, Yussaira; Tachibana, Masato; Kimura, Yui; Kim, Suk; Ichikawa, Yasuaki; Endo, Yasuyuki; Watanabe, Kenta; Shimizu, Takashi; Watarai, Masahisa

    2014-01-01

    Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods, including agglutination and gel diffusion tests. In this study, four recombinant antigens, heat shock protein 60, rhizopine-binding protein, Cu-Zn superoxide dismutase, and hypothetical protein (Ag 4), were constructed. These antigens were coated on latex beads and their usefulness in the serological diagnosis of canine brucellosis was examined. All recombinant antigens showed specific reaction with sera from B. canis-infected dogs in Western blotting. In a microplate agglutination test, mixing sera from B. canis-infected dogs, but not sera from B. canis-free dogs, with single or multiple antigens-coated latex beads produced clear agglutination. Moreover, the antigen-coated latex beads did not show nonspecific agglutination in hemolyzed serum samples. A survey of canine serum samples conducted by the microplate agglutination test using single antigen-coated latex beads showed that this method would be useful in the serological diagnosis of canine brucellosis. Further investigations using more serum samples are required to confirm the usefulness of our method.

  5. Comparative evaluation of recombinant LigB protein and heat-killed antigen-based latex agglutination test with microscopic agglutination test for diagnosis of bovine leptospirosis.

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    Nagalingam, Mohandoss; Thirumalesh, Sushma Rahim Assadi; Kalleshamurthy, Triveni; Niharika, Nakkala; Balamurugan, Vinayagamurthy; Shome, Rajeswari; Sengupta, Pinaki Prasad; Shome, Bibek Ranjan; Prabhudas, Krishnamsetty; Rahman, Habibur

    2015-10-01

    This study aimed to develop latex agglutination test (LAT) using recombinant leptospiral immunoglobulin-like protein (LigB) (rLigB) antigen and compare its diagnostic efficacy with LAT using conventional heat-killed leptospiral antigen and microscopic agglutination test (MAT) in diagnosing bovine leptospirosis. The PCR-amplified 1053-bp ligB gene sequences from Leptospira borgpetersenii Hardjo serovar were cloned in pET 32 (a) vector at EcoRI and NotI sites and expressed in BL21 E. coli cells as fusion protein with thioredoxin (-57 kDa) and characterized by SDS-PAGE and immunoblot. Out of 390 serum samples [cattle (n = 214), buffaloes (n = 176)] subjected to MAT, 115 samples showed reciprocal titre≥100 up to 1600 against one or more serovars. For recombinant LigB protein/antigen-based LAT, agglutination was observed in the positive sample, while no agglutination was observed in the negative sample. Similarly, heat-killed leptospiral antigen was prepared from and used in LAT for comparison with MAT. A two-sided contingency table was used for analysis of LAT using both the antigens separately against MAT for 390 serum samples. The sensitivity, specificity and positive and negative predictive values of recombinant LigB LAT were found to be 75.65, 91.27, 78.38 and 89.96 %, respectively, and that of heat-killed antigen-based LAT were 72.17, 89.82, 74.77 and 88.53 %, respectively, in comparison with MAT. This developed test will be an alternative/complementary to the existing battery of diagnostic assays/tests for specific detection of pathogenic Leptospira infection in bovine population.

  6. Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus.

    Science.gov (United States)

    Trinh, Dai Quang; Ogawa, Haruko; Bui, Vuong Nghia; Nguyen, Tham Thi Hong; Gronsang, Dulyatad; Baatartsogt, Tugsbaatar; Kizito, Mugimba Kahoza; AboElkhair, Mohammed; Yamaguchi, Shigeo; Nguyen, Viet Khong; Imai, Kunitoshi

    2015-09-01

    A blocking latex agglutination test (b-LAT) developed in this study was evaluated for the detection of antibodies against chicken anemia virus (CAV) in chickens. Polystyrene latex beads were coupled with a neutralizing monoclonal antibody (mAb) to CAV (mAb-beads). When mAb-beads were mixed with antigens prepared from the lysate of MDCC-MSB1 cells infected with CAV, agglutination occurred. A short pre-incubation of CAV antigens with CAV-specific antiserum inhibited the agglutination of mAb-beads. The test results were obtained within 5min. The specificity of b-LAT was evaluated using sera from specific pathogen-free chickens and sera containing antibodies to avian influenza virus, Newcastle disease virus, infectious bursal disease virus, and Marek's disease virus; nonspecific agglutination and cross-reactivity with antibodies to unrelated viruses were not observed. The examination of 94 serum samples collected from commercial breeder chickens of various ages (17-63 weeks) revealed good agreement (93.6%, Kappa value=0.82) between b-LAT and a virus neutralization test, known to be most sensitive and specific in the detection of antibodies to CAV. These results indicate that b-LAT, a simple and rapid test, is a useful and reliable tool in CAV serology.

  7. Development and evaluation of a latex agglutination test for the rapid serodiagnosis of tularemia.

    Science.gov (United States)

    Rastawicki, Waldemar; Rokosz-Chudziak, Natalia; Chróst, Anna; Gierczyński, Rafał

    2015-05-01

    A latex agglutination test (LAT) was developed for a rapid detection of antibodies against Francisella tularensis. The assay is performed by mixing serum with antigen-coated latex beads and read within 5 min. Developed LAT has been proved to be a specific, sensitive, fast, easy-to-perform and cost-efficient tool for the routine diagnosis of tularemia.

  8. The comparison of Brucella gel agglutination test with other Brucella tests

    Directory of Open Access Journals (Sweden)

    N. Mine Turhanoğlu

    2015-12-01

    Full Text Available Objective: In this study, it was aimed to compare the sensitivity of diagnostic tests in patients with a preliminary diagnosis of brucellosis. Methods: We have compared the serological methods, standard tube agglutination test (STA, Coombs Test (CT, Rose Bengal (RBT, and the gel centrifugation test. In patients with a preliminary diagnosis of brucellosis, subjects with a positive test result of RBT has been included in the research and other diagnostic tests STA, CT and Coombs Gel centrifugation tests were performed within the range of same titration. Results: Total 132 patient’s serums were studied. In RBT positive 92 patients’ serums, negative test results were found in 11 with STA, in 9 with CT and in 6 with gel test. While 35 patients were identified to be positive by using Brucella gel test at 1/5120 titer, no positive test results were seen with STA and CT at the same titer. Generally, CT results were one titration below the gel centrifugation test results. Conclusion: In conclusion, RBT and STA were not always adequate to determine the diagnosis of brucellosis. Low titer STA results should be supported by tests such as CT or gel centrifugation and the seroconversion must be monitored. Due to giving fast results, gel centrifugation test can be preferred in diagnosis of Brucellosis.

  9. Comparative evaluation of slide agglutination and Widal tube agglutination test in detecting enteric fever among patients attending a tertiary care hospital in North India

    OpenAIRE

    Noor Jahan; Razia Khatoon; Amrita,; Sudhir Mehrotra; Swatantra Kumar

    2016-01-01

    Background: Enteric fever is a major public health problem with significant morbidity and mortality in developing countries. Although, isolation of causative organism from blood is the standard laboratory method, but due to frequent use of self-medication by patients, and its long turnaround time, it is seldom used, and enteric fever is usually diagnosed by using serological methods. Widal tube agglutination test is the standard serological test used, which is now a days replaced by slide agg...

  10. Evaluation of a new latex agglutination test for detection of streptolysin O antibodies.

    OpenAIRE

    Gerber, M. A.; Caparas, L S; Randolph, M F

    1990-01-01

    Acute- and convalescent-phase serum specimens were collected from 50 patients with group A streptococcal pharyngitis. The anti-streptolysin O (ASO) titer for each serum specimen was determined by using both the standard neutralization assay and the latex agglutination (LA) test (Rheumagen ASO; Biokit Inc., New Britain, Conn.). When the ASO titers derived by the two methods were compared, the correlation coefficient was 0.93. When the ability of the LA test to demonstrate a significant ASO tit...

  11. Serotyping of Actinobacillus pleuropneumoniae serotype 5 strains using a monoclonal-based polystyrene agglutination test

    DEFF Research Database (Denmark)

    Dubreuil, J.D.; Letellier, A.; Stenbæk, Eva

    1996-01-01

    A polystyrene agglutination test has been developed for serotyping Actinobacillus pleuropneumoniae serotype 5a and 5b strains. Protein A-coated polystyrene microparticles were sensitized with a murine monoclonal antibody recognizing an epitope on serotype 5 LPS-O chain as shown by SDS......-PAGE and Western blotting, A total of 205 A. pleuropneumoniae, strains including all 12 serotype reference strains and 13 strains representing 8 common bacterial species associated with swine or related to A, pleuropneumoniae, were tested by mixing 25 mu L of polystyrene reagent with the same volume of a dense...... suspension of bacterial cells grown for 18 h. All A, pleuropneumoniae strains had been previously serotyped using standard procedures, The polystyrene agglutination test was rapid (less than 3 min) and easy to perform. Overall a very good correlation (97.3%) with the standard techniques was found...

  12. Serotyping of Actinobacillus pleuropneumoniae serotype 5 strains using a monoclonal-based polystyrene agglutination test

    DEFF Research Database (Denmark)

    Dubreuil, J.D.; Letellier, A.; Stenbæk, Eva;

    1996-01-01

    A polystyrene agglutination test has been developed for serotyping Actinobacillus pleuropneumoniae serotype 5a and 5b strains. Protein A-coated polystyrene microparticles were sensitized with a murine monoclonal antibody recognizing an epitope on serotype 5 LPS-O chain as shown by SDS......-PAGE and Western blotting, A total of 205 A. pleuropneumoniae, strains including all 12 serotype reference strains and 13 strains representing 8 common bacterial species associated with swine or related to A, pleuropneumoniae, were tested by mixing 25 mu L of polystyrene reagent with the same volume of a dense...... suspension of bacterial cells grown for 18 h. All A, pleuropneumoniae strains had been previously serotyped using standard procedures, The polystyrene agglutination test was rapid (less than 3 min) and easy to perform. Overall a very good correlation (97.3%) with the standard techniques was found...

  13. [Yes, we should keep ABO agglutination test within bedside transfusion checks].

    Science.gov (United States)

    Daurat, G

    2008-11-01

    ABO incompatible transfusions are still a frequent cause of serious adverse transfusion reactions. Bedside check is intended to detect patient errors and prevent ABO mismatch. France is one of the few countries that includes ABO agglutination test for red blood cells in bedside checks. Evaluation of this ABO agglutination test, performed with a special card, shows that, on the field, despite frequent users' mishandling, it can detect up to 93% of ABO incompatibilities. This is not enough to rely on this sole test for bedside checks. But, linking it with an another test, currently, checks that the right blood is given to the right patient, rises the sensitivity of the whole bedside procedure up to an estimated 99.65%, for detection of ABO incompatibilities. This linkage has been introduced in the French regulation in 2003. Since then, the incidence of ABO incompatible transfusions has decreased dramatically and faster than in any other country, so France has now, probably, the lowest rate of ABO incompatible transfusions. The investigation of the few ABO accidents that still occur, shows that professionals have always bypassed this linkage. On the other hand, introducing bedside recipient and blood products barcode or radio-chip checks in all the 1500 French hospitals, though technically possible, would provide very little enhancement and lead to major difficulties and expenses. Linkage of ABO agglutination test to patient and blood checks within the bedside procedure has proved to be efficient and should be kept.

  14. A rapid ultrasound particle agglutination method for HIV antibody detection: Comparison with conventional rapid HIV tests.

    Science.gov (United States)

    Bystryak, Simon; Ossina, Natalya

    2017-08-24

    We present the results of the feasibility and preliminary studies on analytical performance of a rapid test for detection of human immunodeficiency virus (HIV) antibodies in human serum or plasma that is an important advance in detecting HIV infection. Current methods for rapid testing of antibodies against HIV are qualitative and exhibit poor sensitivity (limit of detection). In this paper, we describe an ultrasound particle agglutination (UPA) method that leads to a significant increase of the sensitivity of conventional latex agglutination tests for HIV antibody detection in human serum or plasma. The UPA method is based on the use of: 1) a dual mode ultrasound, wherein a first single-frequency mode is used to accelerate the latex agglutination process, and then a second swept-frequency mode of sonication is used to disintegrate non-specifically bound aggregates; and 2) a numerical assessment of results of the agglutination process. The numerical assessment is carried out by optical detection and analysis of moving patterns in the resonator cell during the swept-frequency mode. The single-step UPA method is rapid and more sensitive than the three commercial rapid HIV test kits analyzed in the study: analytical sensitivity of the new UPA method was found to be 510-, 115-, and 80-fold higher than that for Capillus™, Multispot™ and Uni-Gold™ Recombigen HIV antibody rapid test kits, respectively. The newly developed UPA method opens up additional possibilities for detection of a number of clinically significant markers in point-of-care settings. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Comparison of local Salmonella pullorum antigen with imported product in whole blood agglutination test

    Directory of Open Access Journals (Sweden)

    Priyani Medewewa

    Full Text Available Background: Salmonellosis is considered as one of the most important diseases in poultry as it causes devastating losses in chicken industry. Proper identification of the infected and carrier birds is required to control the disease among chickens. In field situation whole blood agglutination test is performed in order to identify carriers of Pullorum and Fowl typhoid particularly, in breeder operations. In this test, serum antibodies are detected by using a specially made antigen for this purpose. In Sri Lanka, three antigen products are used commonly in whole blood agglutination test. Objectives: This study was carried out to compare these two locally available S. Pullorum antigen products and to determine any difference in the efficacy. Methods:“Shaver Brown” commercial layer birds (70 in number were used in the experiment. Birds were inoculated orally with 1.8X109cfu/ml of S. Pullorum at 16 weeks age. After Three weeks post inoculation, blood was collected from each bird and Whole blood agglutination test was performed using both antigen products. Fifteen (15 inoculated hens were selected randomly and cloacal swabs were cultured on cultured Agar on same day of serum collected. Results: In this study, there was no significant difference observed between two antigens to detect carrier birds by whole blood agglutination test. Salmonella was not isolated from cloacal swabs since no observed excretion of Salmonella Pullorum through faces. All cloacal swabs gave negative results, when cultured on artificial Agar. Conclusion: Both antigen can be used effectively to detect carrier birds under the control program in country. [Vet World 2012; 5(9.000: 546-548

  16. Comparison of the Denka Seiken slide agglutination method to the quellung test for serogrouping of Streptococcus pneumoniae isolates.

    Science.gov (United States)

    Shutt, Cheryl K; Samore, Matthew; Carroll, Karen C

    2004-03-01

    This study compared a slide agglutination test (Denka Seiken, Tokyo, Japan) to the "gold standard" quellung reaction (Pneumotest; Statens Serum Institut, Copenhagen, Denmark) for the serogrouping of pneumococci. Two hundred clinical isolates of Streptococcus pneumoniae were used for the comparison. Each assay was performed according to the manufacturer's instructions. There was an overall agreement of 95.7% between the two methods. Only 4 of 10 isolates of serogroup 22 were detected with the slide agglutination assay. Two isolates that were untypeable by the Pneumotest method were typed as serogroups 6 and 31 by the slide agglutination method. The Pneumotest method was unable to type 22 isolates, and the slide agglutination method was unable to type 16 isolates. The slide agglutination method compares favorably with the Pneumotest method and is easier to perform and to interpret.

  17. Comparative evaluation of coagglutination and latex agglutination test (Rotalex kit for detection of rota virus.

    Directory of Open Access Journals (Sweden)

    Mathur M

    1993-07-01

    Full Text Available Coagglutination test was compared with commercially available latex agglutination test (Rotalex kit for detection of rota virus in faecal samples from clinically suspected cases of viral gastroenteritis. Out of 80 test samples 16 (20% and 20 (25.3% were positive for rota virus antigen by Rotalex kit and coagglutination test respectively. All the 40 controls were negative for viral antigen by Rotalex kit and only one gave positive result by coagglutination test. Coagglutination test was found to be economical, sensitive and specific for screening and rapid diagnosis of Rota virus diarrhoea.

  18. Elemental X-ray mapping of agglutinated foraminifer tests: a non- destructive technique for determining compositional characteristics.

    Science.gov (United States)

    Commeau, R.F.; Reynolds, Leslie A.; Poag, C.W.

    1985-01-01

    The composition of agglutinated foraminiferal tests vary remarkably in response to local substrate characteristics, physiochemical properties of the water column and species- dependant selectivity of test components. We have employed a technique that combines a scanning electron microscope with an energy dispersive X-ray spectrometer system to identify major and minor elemental constituents of agglutinated foraminiferal walls. As a sample is bombarded with a beam of high energy electrons, X-rays are generated that are characteristic of the elements present. As a result, X- ray density maps can be produced for each of several elements present in the tests of agglutinated foraminifers. -Authors

  19. Latex agglutination using the periplasmic proteins antigen of Brucella melitensis is a successful, rapid, and specific serodiagnostic test for ovine brucellosis.

    Science.gov (United States)

    Ismael, Alaa Bassuny; Swelum, Ayman Abdel-Aziz; Mostafa, Salama A-H; Alhumiany, Abdel-Rahman A

    2016-09-01

    Brucellosis, especially caused by Brucella melitensis, is considered the most-widespread zoonosis in the world, particularly in developing countries. This study was planned to develop an accurate test for diagnosis of ovine brucellosis using a specific hot saline extracted soluble Brucella melitensis periplasmic proteins (SBPPs). The efficacy of the latex agglutination test (LAT) using SBPPs compared to the Rose Bengal test (RBT), buffered plate agglutination test (BPAT), serum agglutination test (SAT), and an indirect enzyme-linked immunosorbent assay (i-ELISA) was evaluated in the field diagnosis of ovine brucellosis. The test performance was evaluated by estimating sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV), disease prevalence (DP), positive likelihood ratio (PLR), and negative likelihood ratio (NLR) using test agreement and bacteriological culture in 1777 samples. The false-positive result was significantly (P ⩽0.05) lower in LAT than RBT, BPAT, SAT, and i-ELISA. With reference to test agreement, the Se, Sp, PPV, and PLR were highest (P ⩽0.05) in LAT 99.33%, 99.88%, 98.68%, and 827.25%, respectively. With reference to bacteriological culture, the LAT and i-ELISA tests showed a significant difference in Se with SAT. However, no significant difference in specificity was detected. The DP was 8.44% in the five tests. In conclusion, LAT using SBPPs of B. melitensis could be a suitable serodiagnostic field test for ovine brucellosis, with high sensitivity and specificity.

  20. The evolution of pretransfusion testing: from agglutination to solid-phase red cell adherence tests.

    Science.gov (United States)

    Plapp, F V; Sinor, L T; Rachel, J M

    1989-01-01

    Hospital transfusion services and blood centers still use manual hemagglutination tests for most of their serological procedures. Automation of hemagglutination reactions has proven to be difficult, primarily because hemagglutination lacks an objective endpoint which can be easily interpreted by inexpensive instruments. Alternatively, solid-phase red cell adherence assays for ABO cell and serum grouping, Rh typing, red cell and platelet antibody screening, red cell and platelet crossmatching, IgA deficiency screening, hepatitis B surface antigen, and HIV antibody screening have been developed. The performance of these assays compares favorably with current hemagglutination and enzyme immunoassay methods. All of these tests share a common objective endpoint of adherence or nonadherence of indicator red cells. This uniformity allows easy interpretation of results visually, spectrophotometrically, or by image analysis. The latter technique has the potential to revolutionize the reading and interpretation of all agglutination tests. Solid-phase red cell adherence tests in microplates are ideal for batch processing large numbers of specimens. However, adherence tests are not restricted to this format. Therefore, blood grouping dipsticks have been produced, which permit testing of individual blood samples even outside of the laboratory.

  1. Comparison of the latex agglutination test with the hemagglutination inhibition test, enzyme-linked immunosorbent assay, and neutralization test for detection of antibodies to rubella virus.

    OpenAIRE

    Meegan, J M; Evans, B. K.; Horstmann, D. M.

    1982-01-01

    The ability of a rapid, latex agglutination test to diagnose rubella infection and to measure immune status was evaluated by comparison with the hemagglutination-inhibition (HAI) test, enzyme-linked immunosorbent assay (ELISA), and the neutralization (NT) test. The latex agglutination test accurately detected serological conversions in 74 pairs of sera representing 21 natural infections and 53 immunizations. The antibody levels of 276 sera from the general population were determined by latex ...

  2. Establishment Brucella micro agglutination test%布鲁氏菌微量凝集试验的建立

    Institute of Scientific and Technical Information of China (English)

    张双宅

    2012-01-01

    This paper expounded the development of Brucella micro agglutination test methods. At first, we used 96 hole V type blood coagulation plate, 12 tip needle connecting latex drop head for pipetting and then carried out test Now, we used sophisticated 12 adjustable micropipettor, one -time suction head and 96 bore hole type U reaction plate for setting up a convenience, shortcut, time-saving, labor-saving, specimen -saving, reagent - saving brucellosis surveillance and inspection method.%本文阐述了布鲁氏菌微量凝集试验的演变过程,从最初应用96孔V型血凝板、12号去尖针头接乳胶滴头为移液器进行试验,到应用精密的12道可调微量移液器、一次性吸头和96孔一次性U型反应板,建立了一种操作方便、快捷,省时、省力、省标本、省试剂的布鲁氏菌病监测检验方法.

  3. Comparison of PCR, Wright agglutination test and blood culture for diagnosis of brucellosis in suspected patients.

    Science.gov (United States)

    Hekmatimoghaddam, Seyedhosssein; Sadeh, Maryam; Khalili, Mohammad Bagher; Mollaabedin, Mansour; Sazmand, Alireza

    2013-11-15

    Brucellosis has long been prevalent in Iran, with considerable medical and economic importance. Timely diagnosis is needed for early management and effective prevention of its consequences in human beings and animals. Current diagnostic methods impose peculiar challenges in terms of analytical method performance. This study compares diagnostic sensitivity, specificity, predictive Value of Positive (PVP) and Predictive Value of Negative (PVN) for Polymerase Chain Reaction (PCR), Wright agglutination test and blood culture used for patients suspected of brucellosis. In 120 patients clinically suspected of brucellosis and referred by physicians to the Yazd central Medical Laboratory, some relevant demographic, occupational, nutritional and clinical data were collected. Also, venous blood samples were drawn for diagnosis of brucellosis using PCR, Wright agglutination test and blood culture techniques. The most frequent symptom of patients was arthralgia (82 cases, 68.3%). PCR was positive in 25 cases (20.8%), wright test in 21 patients (17.5%) and blood culture in 6 cases (5%). In 20 out of 21 wright-positive cases, PCR was positive and all of the culture-positive patients had positive PCR. Sensitivity, specificity, PVP and PVN of blood culture compared to PCR (as the gold standard test) were 24, 100, 100 and 86%, respectively, but the above parameters when PCR is compared with blood culture (as gold standard) were 100, 83, 24 and 95%, respectively. PCR has better analytical performances than blood culture for diagnosis of brucellosis and is suitable for confirmation of Wright-positive cases.

  4. Diagnostic value of latex agglutination test in diagnosis of acute bacterial meningitis.

    Science.gov (United States)

    Mohammadi, Syeda Fasiha; Patil, Asha B; Nadagir, Shobha D; Nandihal, Namrata; Lakshminarayana, S A

    2013-10-01

    To know the incidence of bacterial meningitis in children below five years of age. To compare conventional culture and antigen detection methods (Latex agglutination test). 100 CSF samples of clinically suspected meningitis cases in children below 5 years of age were included. The samples were subjected to cell count, Gram stain, culture and LAT. The organisms isolated in the study were characterized according to standard procedures. Of the 100 cases studied, 31 cases were diagnosed as ABM by Gram stain, culture and latex agglutination test as per WHO criteria. The hospital frequency of ABM was 1.7%. 15 (48.38) cases were culture positive. Gram stain was positive in 22(70.96) cases and LAT in 17(54.83) cases. Haemophilus influenzae was the most common causative agent of acute bacterial meningitis followed by S.pneumoniae. Case fatality rate was 45.16%. The sensitivity and specificity of LAT was 66.66% and 87.91% respectively. Bacterial meningitis is a medical emergency and early diagnosis and treatment is life saving and reduces chronic morbidity. LAT was more sensitive compared to conventional Gram stain and Culture technique in identifying the fastidious organisms like H.influenzae, S.pneumoniae and Group B Streptococcus. However, the combination of Gram stain, Culture and LAT proved to be more productive than any of the single tests alone.

  5. Diagnostic value of latex agglutination test in diagnosis of acute bacterial meningitis

    Directory of Open Access Journals (Sweden)

    Syeda Fasiha Mohammadi

    2013-01-01

    Full Text Available Objectives: To know the incidence of bacterial meningitis in children below five years of age. To compare conventional culture and antigen detection methods ( Latex agglutination test. Materials and Methods: 100 CSF samples of clinically suspected meningitis cases in children below 5 years of age were included. The samples were subjected to cell count, Gram stain, culture and LAT. The organisms isolated in the study were characterized according to standard procedures. Results: Of the 100 cases studied, 31 cases were diagnosed as ABM by Gram stain, culture and latex agglutination test as per WHO criteria. The hospital frequency of ABM was 1.7%. 15 (48.38 cases were culture positive. Gram stain was positive in 22(70.96 cases and LAT in 17(54.83 cases. Haemophilus influenzae was the most common causative agent of acute bacterial meningitis followed by S.pneumoniae. Case fatality rate was 45.16%.The sensitivity and specificity of LAT was 66.66% and 87.91% respectively. Conclusion : Bacterial meningitis is a medical emergency and early diagnosis and treatment is life saving and reduces chronic morbidity. LAT was more sensitive compared to conventional Gram stain and Culture technique in identifying the fastidious organisms like H.influenzae, S.pneumoniae and Group B Streptococcus. However, the combination of Gram stain, Culture and LAT proved to be more productive than any of the single tests alone.

  6. THE INVESTIGATION OF BRUCELLA ANTIBODY WITH MILK RING TEST AND AGGLUTINATION TEST IN MILK COLLECTED FROM SAMSUN REGION

    Directory of Open Access Journals (Sweden)

    Goknur TERZI

    2006-06-01

    Full Text Available In this study Brucella antibodies were investigated with agglutination test (Whey-AT and Milk Ring Test (MRT in a total of 100 milk samples as 50 of cow milk and 50 of goat milk collected from center and villages of Samsun. According to MRT Brucella antibodies was positive at 10 samples (20 % of cow milk and 6 samples (12 % of goat milk. In cow milk, 4 (8 % positive, 3 (6 % suspicious and 43 (86 % negative samples; in goat milk 3 (6 % positive, 2 (4 % suspicious and 45 (90 % negative samples were determined according to antibodies titre of serum agglutination test (Whey-AT. [TAF Prev Med Bull 2006; 5(3.000: 196-203

  7. Detection of acute childhood meningitis by PCR, culture and agglutination tests in Tabriz, Iran.

    Science.gov (United States)

    Ghotaslou, Reza; Farajnia, Safar; Yeganeh, Fatemeh; Abdoli-Oskouei, Shahram; Ahangarzadeh Rezaee, Mohammad; Barzegar, Mohammad

    2012-01-01

    Meningitis is one of the hazardous and life threatening infections and is associated with mortality and morbidity. The aim of this study was to determine etiological agents of childhood bacterial meningitis. The culture, Gram staining, agglutination and PCR assays were used to examine CSF specimens from 277 patients with presumed bacterial meningitis for the occurrence of 4 most common infectious agents consist of N. meningitis, H. influnsae, S. pneumoniae and S. agalactiae between 2008 and 2009 at different wards of the Children Hospital of Tabriz. The mean age of patients was 35 ± 2 (Mean ± SEM) month, (minimum 11 days maximum 14 years), of all cases 59.6% male and 40.4% female. Overall the diagnosis was confirmed with a CSF culture in 11/277 (3.97%), by agglutination test in 14/277 (5.05%). The isolated bacteria included S. pneumoniae 5 cases, H. influnsae 2 cases, N. meningitis 3 cases and P. aeroginusae 1 case. A positive PCR assay allowed us to diagnose bacterial meningitis in 19 patients (6.8%). In the present study, we found PCR to be a useful and sensitive method for the detection of bacterial DNA in the CSF samples from suspected meningitis patients. Furthermore, to maximize management of meningitis cases, a combination of culture and PCR is necessary.

  8. Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia

    NARCIS (Netherlands)

    Piek, C.J.; Teske, E.; van Leeuwen, M.W.; Day, M.J.

    2012-01-01

    Abstract Background The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). Methods Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two

  9. Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia

    NARCIS (Netherlands)

    Piek, C.J.; Teske, E.; van Leeuwen, M.W.; Day, M.J.

    2012-01-01

    Abstract Background The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). Methods Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories

  10. Latex particle agglutination test as an adjunct to the diagnosis of bacterial meningitis

    Directory of Open Access Journals (Sweden)

    Surinder K

    2007-01-01

    Full Text Available The present study aimed to review the results of microscopic examination, routine culture and antigen detection by latex particle agglutination test (LPAT, in order to evaluate the diagnostic value of the LPAT in establishing the aetiological diagnosis of bacterial meningitis. LPAT was done in 65 clinically suspected meningitis cases ranging from 5 days to 60 years of age and was compared with culture and Gram stain. Using LPAT, an aetiological diagnosis could be done in 10 out of 65 (15.4% cases of bacterial meningitis. In contrast, Gram stain and culture showed 16.9 and 23.1% positivity, respectively. LPAT correlated well with Gram stain and culture and can be recommended as an adjunct laboratory test for rapid aetiological diagnosis of bacterial meningitis for prompt institution of proper antibiotics.

  11. Use of the Directigen Latex Agglutination Test for Detection of Haemphilus influenzae, Streptococcus pneumoniae, and Neisseria meningitidis Antigens in Cerebrospinal Fluid from Meningitis Patients,

    Science.gov (United States)

    Reprint: Use of the Directigen Latex Agglutination Test for Detection of Haemphilus influenzae, Streptococcus pneumoniae , and Neisseria meningitidis Antigens in Cerebrospinal Fluid from Meningitis Patients.

  12. The clinical significance comparison of a latex agglutination based syphilis screening test at low antibody titer.

    Science.gov (United States)

    Wang, Hua-Cheng; Chen, Cha; Wang, Li-Na; Long, Yi-Fei; Zhang, Wei-Zheng; Li, You-Qiang; Xiao, Qian; Yuan, Hui

    2013-01-01

    The rapid increase of syphilis underscores a tremendous need to carefully evaluate many new serological tests for syphilis and choose efficient and economical strategies for syphilis screening, especially in the case of primary infection with low antibody titer. Between 2011 and 2012, 73 patients' sera samples were included in this retrospective study. They were either TRUST or TPPA reactive, either LA (latex agglutination) based auto3 TP or CLIA (chemiluminescence assay) based Architect Syphilis TP assay reactive. The contradictory weak response samples were further examined by FTA-Abs method. TPPA could not give reactive results in samples with antibody concentration less than 10 mIU. Auto3 TP reagent shows good linearity at low antibody titers and was more sensitive than TPPA, while the former does not show significant superiority compared to the Architect Syphilis TP assay at low antibody titer, except that it is suitable for adaptation on diverse automated chemistry analyzers.

  13. Seroprevalence of bovine leptospiral antibodies by microscopic agglutination test in Southeast of Iran

    Institute of Scientific and Technical Information of China (English)

    Mohammad Khalili; Ehsanollah Sakhaee; Mohammad Reza Aflatoonian; Gholamreza Abdollahpour; Saeed Sattari Tabrizi; Elham Mohammadi Damaneh; Sajad Hossini-nasab

    2014-01-01

    Objective:To evaluate serological findings of bovine leptospirosis which is a zoonotic disease with worldwide distribution caused by Leptospira interrogans. Methods: One hundred and sixty seven sera were collected from 9 commercial dairy herds in jiroft suburbs, from July to October 2011. Microscopic agglutination test (MAT) was used to evaluates serological findings of bovine leptospirosis in Jiroft suburb dairy farms, Kerman province, Iran. Results:Antibodies were found by MAT at least against one serovar of Leptospira interrogans in 29 samples (17.36%) among 167 sera at a dilution 1:100 or higher, and Leptospira pomona was the most prevalent serovar. Positive titers against more than one serovar were detected in 6 sera of the positive samples. Conclusion:This study is the first report of leptospirosis in Southeast Iran and showed that Leptospira pomona was the most and Leptospira icterohaemorrhagiae the least prevalent serovars in Southeast Iran.

  14. Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-screen latex agglutination test

    NARCIS (Netherlands)

    W.B. van Leeuwen (Willem); C. van Pelt (Cindy); A. Luijendijk (Ad); H.A. Verbrugh (Henri); W.H.F. Goessens (Wil)

    1999-01-01

    textabstractThe slide agglutination test MRSA-Screen (Denka Seiken Co., Niigata, Japan) was compared with the mecA PCR ("gold standard") for the detection of methicillin resistance in Staphylococcus aureus. The MRSA-Screen test detected the penicillin-binding protein 2a

  15. The Expression and Characterization of a Bifunctional Protein in E. coli for Autologous Erythrocyte Agglutination Test

    Institute of Scientific and Technical Information of China (English)

    Changli Shao; Jingang Zhang

    2008-01-01

    H antigen, the precursor of A and B antigens, belongs to Hh blood system in which it is the only antigen. H antigen distributes on all the human RBC surface except for Bombay phenotype and the copy number of H antigen on the surface of an adult RBC is approximately 1.7 x 106. These characteristics made H antigen the potential target molecule for the immunoassay and immunotherapy. A monoclonal antibody 2E8 against H antigen on the surface of erythrocyte had been prepared in previous work. Based on this antibody, the variable region genes of heavy and light chains (VH and VL) from 2E8 had been cloned by 5' RACE. The two variable region genes were spliced by overlap extension and assembled ScFv (VH-linker-VL) gene encoding the anti-H antigen named ScFv2EB. According to the prediction of the three-dimension structure of ScFv2EB and CH1 fragment from 2E8 and HIV-1 gp41 antigen peptide, we further constructed the ScFv2EBCH1-gp41 fusion molecule. The recombinant ScFv2EB-CH1-gp41 gene was cloned into pET-his vector and expressed in BL21(DE3)plysS cells. The fusion protein was purified from the inclusion bodies. In a series of subsequent analyses, this fusion protein showed identical antigen binding site and activity with the parent antibody. Meanwhile, in mimic test, as the main ingredient of reagent for autologous erythrocyte agglutination test, the bifunctional protein could agglutinate the RBCs in the presence of HIV-1 gp41 antibodies using sera from HIV-infected individuals. Cellular & Molecular Immunology. 2008;5(4):299-306.

  16. THE INVESTIGATION OF BRUCELLA ANTIBODY WITH MILK RING TEST AND AGGLUTINATION TEST IN MILK COLLECTED FROM SAMSUN REGION

    OpenAIRE

    Goknur TERZI

    2006-01-01

    In this study Brucella antibodies were investigated with agglutination test (Whey-AT) and Milk Ring Test (MRT) in a total of 100 milk samples as 50 of cow milk and 50 of goat milk collected from center and villages of Samsun. According to MRT Brucella antibodies was positive at 10 samples (20 %) of cow milk and 6 samples (12 %) of goat milk. In cow milk, 4 (8 %) positive, 3 (6 %) suspicious and 43 (86 %) negative samples; in goat milk 3 (6 %) positive, 2 (4 %) suspicious and 45 (90 %) negativ...

  17. Microbead agglutination based assays

    KAUST Repository

    Kodzius, Rimantas

    2013-01-21

    We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.

  18. Development of a Specific Latex Agglutination Test to Detect Antibodies of Enterovirus 71.

    Science.gov (United States)

    Qin, Bo; Zhang, Jianhua; Xie, Wenhao; Liu, Xuehong; He, Tingting; Chen, Jinkun; Dong, Xuejun

    2015-10-01

    A latex agglutination test (LAT) was developed for the rapid detection of antibodies against the VP1 or VP1 proteins of Enterovirus 71 (EV71). The proteins of interest including prokaryotically expressed VP1 and two strains of anti-VP1 monoclonal antibody (McAb) against EV71 were covalently linked to carboxylated latex using ethyl-dimethyl-amino-propyl carbodiimide (EDC) to prepare sensitized latex beads. LAT was evaluated by an enzyme-linked immunosorbent assay (ELISA) as a reference test. The VP1-LAT showed a sensitivity of 87.0%, specificity of 88.9%, and an agreement ratio of 90.0% in detecting VP1 in 100 serum samples from experimentally infected mice, whereas these values were 86.8, 96.7, and 93.3%, respectively, for 608 clinical human serum samples. The VP1-LAT has advantages over other assays in terms of low cost, rapidity, chemical stability, high sensitivity, repeatability, and specificity. The LAT established in the present study is a rapid and simple test suitable for field monitoring of antibodies against VP1-EV71.

  19. Validation studies of the latex agglutination test for the detection of Trichinella larvae in meat products.

    Science.gov (United States)

    Gayda, Jennifer; Reckinger, Sabine; Thaben, Nora; Nöckler, Karsten; Mayer-Scholl, Anne

    2016-11-15

    Human trichinellosis is a foodborne disease caused by ingestion of meat infected with Trichinella muscle larvae. To control Trichinella spp. infection in the European Union, all slaughtered pigs from holdings that are not officially recognized as applying controlled housing conditions and other animals susceptible to Trichinella infection and intended for human consumption should be examined by one of the approved digestion methods described in Regulation (EU) No. 2015/1375. In the past, Trichinella outbreaks due to the consumption of cured wild boar or pork products have been described in several European countries, making the identification of the larvae from these products relevant for Trichinella control. Therefore, this study aimed to validate the newly approved latex agglutination test (Trichin-L) for routine testing of cured meat products. The test was validated based on the OIE Guidelines using pork products spiked with Trichinella larvae. The sensitivity of the method varied greatly depending on the investigated meat product and was usually lower than for the gold standard, the magnetic stirrer method. The detection rate reached 80% for three larvae and 60% for one larva in cured pork sausages. A detection rate of 100% for three larvae and 50% for one larva was found in bacon. For frozen samples (-20°C) the Trichin-L kit is similarly sensitive as for cured samples. Further, to determine the performance of the test under field conditions, pork products from regions with known high Trichinella prevalences confiscated by customs authorities at two German international airports were analyzed. Problems associated with the Trichin-L test were incomplete digestion due to fatty ingredients, spices and very dry meat products, resulting in data which could not be evaluated. Therefore, the test is currently not suitable for the detection of Trichinella larvae in cured meat products and needs further adaptation steps to increase both usability and sensitivity

  20. Typhoid fever in a Tertiary Hospital in Nigeria: Another look at the Widal agglutination test as a preferred option for diagnosis.

    Science.gov (United States)

    Enabulele, Osahon; Awunor, Simeon Nyemike

    2016-01-01

    Single Widal agglutination test rather than blood culture, is commonly employed to diagnose typhoid fever in Nigeria. We took another look at the Widal agglutination test as a preferred option for diagnosis of typhoid fever by determining the specificity and sensitivity of Widal agglutination test in febrile adult patients. Two hundred and seventy-one blood samples from consecutive adults (>18 years) with febrile illness attending the General Practice Clinic of the University of Benin Teaching Hospital were tested using the Widal agglutination test, blood culture, and malaria parasite test on each sample to establish the diagnosis of typhoid fever. Of the 271 blood samples 124 (45.76%) were positive following a Widal agglutination test, 60 (22.10%) blood samples grew Salmonella organisms on blood culture while 55 (20.29%) blood samples showed a co-infection of typhoid fever and malaria. A sensitivity of 35%, specificity of 51%, positive predictive value of 17%, and a negative predictive value of 73% were observed for Widal agglutination test as a diagnostic modality for typhoid fever infection. A single Widal agglutination test is not a valid diagnostic option for typhoid fever while co-infection with malaria parasite is the preponderant microbiological finding in typhoid fever infections. The severity of malaria parasitemia is associated with positive titers on Widal test.

  1. Detection of leptospiral antibodies by microscopic agglutination test in north-east of Iran

    Institute of Scientific and Technical Information of China (English)

    Ehsanollah Sakhaee; Gholam Reza Abdollah pour

    2011-01-01

    Objective: To detect leptospiral antibodies by microscopic agglutination test (MAT) in north-east of Iran. Methods: This study was conducted to evaluate prevalence of human leptospiral infections by MAT, using six current reference strains of Leptospira interrogans in north-east of Iran. A total of 285 serum samples were collected from three north-east provinces of Iran, from December, 2009 to June, 2010. Results: Antibodies were detected at least against one serovar of Leptospira interrogans in 45 sera (15.79 %) among 285 samples at a dilution 1:100 or greater. Positive titers against more than one serovar were detected in 24 sera of the positive samples. Therefore, there were 75 positive reactions against different serovar of Leptospira interrogans. Positive titers were recorded against serovar icterohaemorrhagiae (31 samples), hardjo (26 samples), grippotyphosa (7 samples), pomona (5 samples), canicola (4 samples) and ballum (2 sample).Conclusions:In present study the most prevalent (Leptospira icterohaemorrhagiae) and the least prevalent (Leptospira ballum) serovar are different from previous studies. Maybe, species and prevalence of serovars change during the time in one area and between regions.

  2. Evaluation of an Immunocapture-Agglutination Test (Brucellacapt) for Serodiagnosis of Human Brucellosis

    Science.gov (United States)

    Orduña, Antonio; Almaraz, Ana; Prado, Ana; Gutierrez, M. Purificación; Garcia-Pascual, Agustina; Dueñas, Ana; Cuervo, Milagros; Abad, Ramon; Hernández, Beatriz; Lorenzo, Belen; Bratos, Miguel A.; Torres, Antonio Rodriguez

    2000-01-01

    We evaluated the validity and the usefulness of a new test for the diagnosis of human brucellosis based on an immunocapture-agglutination technique. A total of 315 sera from 82 patients with a diagnosis of brucellosis, 157 sera from patients in whom brucellosis was suspected but not confirmed, and 412 sera from people living in rural areas with endemic brucellosis were studied. The seroagglutination test (SAT), Coombs anti-Brucella test, and Brucellacapt test were evaluated. All the initial sera from the 82 patients proved to be positive in Brucellacapt and Coombs tests, while only 75 (91.4%) were positive in the SAT. If a ≥1/160 diagnostic threshold titer was defined for the Brucellacapt test, Coombs test, and SAT, the sensitivities were 95.1, 91.5, and 65.8%, respectively. Taking the same diagnostic threshold titer for the 157 sera from the unconfirmed but suspected patients, the specificities of the Brucellacapt, Coombs, and SAT were 81.5, 96.2, and 100%, respectively; for the 412 control sera, the specificities were 99.0, 99.8, and 100%. The diagnostic efficiency (area below the receiver operating characteristic curve) of Brucellacapt was 0.987852 (95% confidence interval [CI], 0.95109 to 0.99286), very similar to the diagnostic efficiency of the Coombs test (0.97611; 95% CI, 0.94781 to 0.99146) and higher than that of SAT (0.91013; 95% CI, 0.86649 to 0.94317). The results of the Brucellacapt test were compared with those of the Coombs test (correlation coefficient, 0.956; P = 0.000) and SAT (correlation coefficient, 0.866; P = 0.000). The study shows very good correlation between the Brucellacapt and Coombs tests, with a high concordance between titers obtained in the two tests. Nevertheless, lower correlation and concordance were found between the Brucellacapt and Coombs tests when the results for titers of ≥1/160 were compared (0.692; P = 0.000). In acute brucellosis, the Brucellacapt and Coombs tests render positive titers of ≥1/160. When the titers

  3. Evaluation of latex agglutination tests for fibrin-fibrinogen degradation products in the forensic identification of menstrual blood.

    Science.gov (United States)

    Akutsu, Tomoko; Watanabe, Ken; Motani, Hisako; Iwase, Hirotaro; Sakurada, Koichi

    2012-01-01

    The identification of menstrual blood is important when discriminating menstruation from vaginal trauma in sexual assault cases. The aim of this study was to evaluate two fibrin-fibrinogen degradation product (FDP)-latex agglutination test kits, FDPL® Test (FDP-L) and FDP Plasma "RD" (FDP-P), for their ability to forensically identify menstrual blood. Sensitivity and specificity of the two kits were compared for menstrual blood and various body fluids, and the sensitivity of the FDP-latex agglutination test kit was also compared with that of an immunochromatographic test for human hemoglobin. The robustness of the FDP-latex agglutination test was compared with that of gene expression analysis of menstrual blood specific markers. The FDP-L kit was more sensitive than the FDP-P kit, but it cross-reacted with peripheral bloodstains from healthy volunteers. The FDP-P kit was specific for menstrual blood, with the exception of postmortem blood samples, and was not affected by other body fluids. In an FDP-negative menstrual blood sample, the sensitivity of human hemoglobin detection was lower than for FDP-positive samples and peripheral blood stains, suggesting that determination of human hemoglobin could be useful in interpreting negative results in the FDP-latex agglutination test. In menstrual blood samples incubated in wet conditions, FDP was found to be a robust marker in the identification of menstrual blood compared with mRNA markers. FDP-P testing was shown to be a suitable and highly efficient rapid screening test for the laboratory identification of menstrual blood.

  4. Analysis of 1926 Samples of Brucellosis Serological Agglutination Test%1926份布鲁氏菌病血清学凝集试验结果分析

    Institute of Scientific and Technical Information of China (English)

    姜勇波; 李晓红; 孙宗祥; 孙力; 冯波

    2015-01-01

    In this paper,through the comparison of Rose Bengal plate agglutination test(RBPT)and serum agglutination test(SAT)in the diagnosis ofbrucel osis sensitivity and accuracy,draw a conclusion:Brucel a diagnosis in the application of two methods,two methods used at the same time can improve the detection rate of brucel osis,not a single confirmed by a method,and contact must be combined with epidemiological and clinical symptoms and signs seriously analysis,careful judgment.%本文通过比较虎红平板凝集试验(RBPT)和试管凝集试验(SAT)在诊断布鲁氏菌病的敏感性和准确性,得出结论:在应用两种方法进行布病诊断时,两种方法同时使用可提高布鲁氏菌病的检出率,不能单一通过一种方法就确诊,必须结合流行病学接触史和临床症状体征认真分析谨慎判断。

  5. [Relationship between the sensitivity of the delayed agglutination test and synthetic detergents].

    Science.gov (United States)

    Iovchev, E; Vodas, K

    1977-01-01

    Residual amounts of detergents (Losk, Bio-73, Alka-lux, Bourgas, Bourgaslux, and Vero) in a concentration of 10-5 to 10-7 in physiologic saline can inhibit the agglutination titers by 3 to 5 degrees. This could mislead in the assessment of the reaction with regard to its diagnostic value. It is admitted that the inhibition produced is due to changes in the antibodies--drop in the total protein and light variations in all protein fractions as well as in the probable surface deterioration of the antigen, leading to its defective agglutinability. It is suggested to rinse more than five times all glassware that has been cleaned with detergents.

  6. New approach for serological testing for leptospirosis by using detection of leptospira agglutination by flow cytometry light scatter analysis.

    Science.gov (United States)

    Yitzhaki, S; Barnea, A; Keysary, A; Zahavy, E

    2004-04-01

    Leptospirosis is considered an important reemerging infectious disease worldwide. The standard and most widespread method for the diagnosis of leptospirosis is the microscopic agglutination test (MAT). This test is laborious and time-consuming, and the interpretation of the results is subjective. In the present work we describe an application of flow cytometry (FCM) as a tool for the serological diagnosis of leptospirosis. The analysis is based on the sensitivity of FCM to the size and shape of the bacteria analyzed by measurement of light scatter parameters: forward scatter (FSC) and side scatter (SSC). The addition of positive serum to an infecting leptospiral serovar results in a shift of the light scatter parameter to a different location with higher FSC and SSC values, indicating the formation of leptospiral aggregates. By using immunofluorescent staining, we have shown that the large particles formed are the agglutinated leptospires. Quantification of the agglutination process has been achieved by calculating an agglutination factor (Af), based on changes in the light scatter parameters measured by FCM. Af enables us to determine the specificity of the serological reaction of the patient serum with each leptospiral serovar. In this work, 27 serum samples from 18 leptospirosis patients were tested by both the MAT and the FCM techniques, in which each serum sample was tested against 13 serovars. Twenty-six human serum samples derived from patients with a variety of other defined illnesses were used as negative controls and enabled us to define the Af threshold value as < 9.3 for negative patients, while any value higher than that would be a positive result for leptospirosis. Compared to MAT, the FCM technique was found to be more specific and sensitive, especially in identifying the serogroup in the acute phase of the disease. The whole process was found to be rapid and took less than 1.5 h. Moreover, FCM analysis is objective and can be automated for the

  7. [Detection of mutans streptococci by latex agglutination test and its application as a caries-activity test].

    Science.gov (United States)

    Takei, T

    1990-06-01

    The number of mutans streptococci in saliva and dental plaque has been reported to correlate with the incidence of dental caries. This report describes a simple and rapid diagnostic method for the detection of mutans streptococci in dental plaque using latex agglutination (LA) test. Latex particles (0.876 microns, diameter) were sensitized with partially purified antibodies against whole cells of Streptococcus mutans MT8148 (c), MT703R (e) and OMZ175 (f) and Streptococcus sobrinus B13 (d) and 6715 (g). Whole cells of mutans streptococci or dental plaque was extracted with a mixture of 8M sodium nitrite (5 microliters) and 2M acetic acid (5 microliters) for five minutes and neutralized with 2M sodium hydroxide (10 microliters), and the extract and the sensitized latex suspension (20 microliters) were mixed and the grade of agglutination reaction was read macroscopically after ten minutes standing at room temperature. The LA tests could detect 1.0 10ng of purified serotype antigen and 10(5)-10(6) CFU of live cells of mutans streptococci and specifically distinguish not only the mutans streptococci from the other streptococci but also S. mutans from S. sobrinus. However, cross-reactions were still observed among the serotypes c, e and f of S. mutans or between the serotypes d and g of S. sobrinus. Plaque samples were collected from 168 children (2 to 12 years of age) and the 0.1 mg (wet weight) were applied to the LA tests. At the same time, the total number of mutans streptococci in plaque and the serotypes of each isolate were determined. The results of LA reaction correlated significantly with the number of mutans streptococci in plaque (chi-square analysis; p less than 0.0001). The LA tests discriminated between S. mutans and S. sobrinus in dental plaque. It was found that the latex particles sensitized with anti-serotype c and/or e S. mutans antibodies were most effective in demonstrating mutans streptococci, and they were used in the following studies. The

  8. Seroepidemiological detection of antibodies against Leptospira spp using microscopic agglutination test in Urmia cows and sheep

    Directory of Open Access Journals (Sweden)

    Ramin Ag

    2013-01-01

    Full Text Available The study was designed to determine the level of incidence, titer and various serovars of leptospira in 203 cows and 166 sheep at Urmia abattoir in 2011. Blood samples were collected during the slaughter of animals and sera were separated to evaluate the serological reaction to Leptospira spp by Microscopic Agglutination Test (MAT using live antigens representing Leptospira interrogans serogroups: pomona, grippotyphosa, canicola, hardjo, icterrohaemoragiae, and ballum. Overall, 36% of cows and 19.3% of sheep including 33.8% of bulls, 40.5% of female cows, 18.3% of rams and 25% of ewes had a positive reaction to at least one of the leptospira serovars. The most prevalent serovars in cows were pomona (22.7%, grippotyphosa (13.8%, and hardjo (8.4%, and in sheep were grippotyphosa (66.7%, pomona (26.2% and canicola (7.1%. Other serovars were not detected in cows and sheep. The most prevalent serological titers of 1:100 and 1:200 in cows was 18.2% and 26.6%, and for sheep were 13.5% and 8%, respectively, and of 1:400 in sheep was 2.3%. Cows with a positive reaction to one, two and three serovars were 28.6%, 5.9%, and 1.5% and sheep positive to one and two serovars were 13.3% and 6%, respectively. Age comparison in seropositive cows and sheep showed a significantly increased infection (p<0.05 from young to adult ruminants, while no differences were seen regarding gender. The main mixed serovars were between grippotyphosa/pomona, grippotyphosa/canicola and canicola/pomona. The gender comparison of the serovars' distribution revealed that the pomona and grippotyphosa were predominant among other leptospiral serovars in cows and sheep, respectively. In conclusion, the rate of leptospirosis in Urmia cows was about 2 fold in sheep. The most current serovars in cows and sheep were pomona and grippotyphosa, respectively. The majority of animals was infected with one serovar, but polyserovars, are also possible. The highest titer (1:200 was observed in cows

  9. Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia

    Directory of Open Access Journals (Sweden)

    Piek Christine J

    2012-02-01

    Full Text Available Abstract Background The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT for the diagnosis of immune-mediated haemolytic anaemia (IMHA. Methods Canine (n = 247 and feline (n = 74 blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA. Results The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA. Conclusions The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT.

  10. Evaluation of the MycoAKT latex agglutination test for rapid diagnosis of Mycobacterium avium complex infections.

    Science.gov (United States)

    Olano, J P; Holmes, H; Woods, G L

    1998-01-01

    Rapid diagnosis of Mycobacterium avium complex (MAC) bacteremia is important for management of patients with the acquired immunodeficiency syndrome who have disseminated MAC. The purpose of this study was to determine the reliability of the MycoAKT latex agglutination test for direct detection of MAC in positive mycobacterial blood cultures. First, colonies of isolates of previously identified mycobacteria, including 35 MAC, were tested. Of the 55 isolates evaluated, 33 were identified as MAC by the latex test, including 31 of the known MAC and 2 M. chelonae (sensitivity, 88.6%; specificity, 90.0%). Second, broth from 20 ESP II and 20 BACTEC 12B bottles seeded with isolates of MAC were tested. Aliquots from 19 (95%) ESP II cultures and 16 (80%) 12B cultures were positive by the latex test. In phase 3, broth from 115 signal-positive ESP II blood cultures were tested by latex agglutination. Forty-three subcultures from these bottles grew mycobacteria (41 MAC and 2 Mycobacterium tuberculosis complex); the remainder grew no organisms. Broth from 40 of the blood cultures (39 that grew MAC and 1 from which no organisms were recovered) were latex positive; thus, the sensitivity, specificity, and positive and negative predictive values of the latex test for direct identification of MAC in ESP II blood cultures were 95.1, 98.6, 97.5, and 97.3%, respectively. The mean time to detection of MAC was 14.6 days (range, 6-34 days) with the direct latex test, compared with 18.3 days (range, 9-36 days) with subculture and probe (p < 0.05).

  11. A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis.

    Science.gov (United States)

    Oliveira, Edward; Saliba, Juliana Wilke; Oliveira, Diana; Dias, Edelberto Santos; Paz, Gustavo Fontes

    2016-05-15

    This report describes the stege I/II development of a new direct agglutination test (DAT) for the diagnosis of canine visceral leishmaniasis (CVL) using freeze-dried antigen produced Coomassie blue-stained Leishmania (Leishmania) infantum promastigotes. In stage I, 16 canine serum samples, collected from eight dogs carrying CVL and eight healthy dogs, were assessed with the DAT using 2-mercaptoethanol (2-ME), N-acetyl-cysteine (NAC), kaolin or NAC plus urea (NAC+U) to improve the assay conditions. Stage II assessed the diagnostic accuracy with 100 serum samples collected from dogs with symptomatic CVL and clinically healthy dogs, comparing the four different sample diluents. The CVL-DAT prototype kit showed equivalent performances when 2-ME, NAC or NAC+U were used: 97.1% sensitivity (CI: 83-99.8%), 97% specificity (CI: 88.5-99.5%) and a 97% diagnostic accuracy (CI: 90.8-99.2). With kaolin, a 94.1% sensitivity (CI: 79-99%), 97% specificity (CI: 88.5-99.5%) and 96% diagnostic accuracy were observed (CI: 89.5-98.7), with no statistically significant differences among the four reagents (p=1.0). The NAC plus urea in sample diluent decreased non-specific agglutination, promoted a better defined sharp-edged blue spot and was thus chosen as a component for the new DAT prototype to diagnose canine VL, designated DAT-Canis.

  12. ELISA Cut-off Point for the Diagnosis of Human Brucellosis; a Comparison with Serum Agglutination Test

    Directory of Open Access Journals (Sweden)

    Anahita Sanaei Dashti

    2012-03-01

    Full Text Available Background: Brucellosis is a world-wide disease, which has a diverse clinical manifestation, and its diagnosis has to be proven by laboratory data. Serum agglutination test (SAT is the most-widely used test for diagnosing brucellosis. The enzyme linked immunosorbent assay (ELISA can also determine specific antibody classes against brucella. It is a sensitive, simple and rapid test, which could be an acceptable alternative to SAT with fewer limitations, however, like any other new test it should be further evaluated and standardized for various populations. This study was planned to determine an optimal cut-off point, for ELISA which would offer maximum sensitivity and specificity for the test when compared to SAT.Methods: Four hundred and seven patients with fever and other compatible symptoms of brucellosis were enrolled in the study. Serum agglutination test, 2-Mercaptoethanol test, and ELISA were performed on their sera. Results: The cut-off point of 53 IU/ml of ELISA-IgG yielded the maximal sensitivity and specificity comparing to the other levels of ELISA-IgG, and was considered the best cut off-point of ELISA-IgG to diagnose acute brucellosis. At this cut-off, the sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio were 84.09%, 85.38%, 62.20, 94.90, 5.75, 0.18, respectively.Conclusion: The best cut-off point of ELISA-IgG is 53 IU/ml, which yields the maximal sensitivity and specificity to diagnose acute brucellosis.

  13. A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF

    Directory of Open Access Journals (Sweden)

    ROCHA Sérgio M.

    2002-01-01

    Full Text Available Simple and rapid latex-based diagnostic tests have been used for detecting specific antigens or antibodies in several diseases. In this article, we present the preliminary results obtained with a latex agglutination test (LAT for diagnosing neurocysticercosis by detection of antibodies in CSF. A total of 43 CSF samples were assayed by the LAT: 19 CSF samples from patients with neurocysticercosis and 24 CSF samples from patients with other neurologic disorders (neurosyphilis, n = 8; neurotoxoplasmosis, n = 3; viral meningitis, n = 4, chronic headache, n = 9. The LAT exhibited 89.5% sensitivity and 75% specificity. The use of LAT seems to be an additional approach for the screening of neurocysticercosis with advantage of simplicity and rapidity. Further studies could be performed using purified antigens and serum samples.

  14. The Puzzle of the False Positve Reactions in Cr. Neoformanse’s Capsular Antigen Slide Lates Agglutination Test by Sera of Patients with Rheumatoid Arthritis

    Directory of Open Access Journals (Sweden)

    F. Zaini

    1987-07-01

    Full Text Available Different examination have shown that rheumatoid factor is not responsible for false positive reaction (F.P.R. in Cr. Neoformanse’s free capsular antigen latex agglutination test, whereas high levels of iron in sera of patients with rheumatoid arthritis as well as other sera was responsible not only for this F.P.R. , but also had an important role in the production of F.P.R. in many slide latex agglutination tests. This is because of Iron’s Ion reaction with reagent’s preservative: sodium azid and/or negative charge of the antigens fixed to the latex particles.

  15. Bayesian estimation of sensitivity and specificity of the modified agglutination test and bioassay for detection of Toxoplasma gondii in free-range chickens

    Science.gov (United States)

    Toxoplasma gondii infects virtually all warm-blooded animals worldwide. Serological tests, including the modified agglutination test (MAT), are often used to determine exposure to the parasite. The MAT can be used for all hosts because it does not need species-specific reagents and has been shown to...

  16. Comparison of Brucella immunoglobulin M and G flow assays with serum agglutination and 2-mercaptoethanol tests in the diagnosis of brucellosis

    NARCIS (Netherlands)

    A. Zeytinoglu; A. Turhan; I. Altuglu; A. Bilgic; T.H. Abdoel; H.L. Smits

    2006-01-01

    The diagnostic value of Brucella IgM/IgG flow assays was evaluated in comparison with serum agglutination and 2-mercaptoethanol tests by testing a selection of serum samples submitted to the laboratory because of clinical suspicion of brucellosis. All 39 admission and 11 follow-up samples that agglu

  17. Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola

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    Fabio Hiroto Shimabukuro

    2013-08-01

    Full Text Available Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect in a microscopic agglutination test (MAT. This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.

  18. Evaluation of latex agglutination test and oxacillin resistant screening agar base (ORSAB medium for the detection of oxacillin resistant coagulase negative Staphylococci (ORCoNS (Preliminary study

    Directory of Open Access Journals (Sweden)

    Sjoekoer M. Dzen

    2007-12-01

    Full Text Available Coagulase negative Staphylococci (CoNS are recognized as an important cause of nosocomial infection, especially in neonates and patients with indwelling prosthetic devices. The CoNS resistance rate to oxacillin has been increasing. Therefore, rapid and accurate detection of oxacillin resistance is essential in order to determine the most appropriate antimicrobial therapy. This study aimed to prove that latex agglutination test and oxacillin resistant screening agar base (ORSAB medium can be used for rapid detection of oxacilllin resistant CoNS (ORCoNS. Latex agglutination test and ORSAB medium compared with the conventional method was conducted in this study toward 30 clinical isolates of CoNS for the detection of ORCoNS. Mc Nemar test was used to analyze the data. The study result revealed that there was no significant difference (P>0.05 in terms of ORCoNS detection between the latex agglutination test and ORSAB medium on the one hand, and the conventional method on the other. It is concluded that latex agglutination test and ORSAB medium can be used for rapid detection of ORCoNS. (Med J Indones 2007; 16:228-32Keywords: nosocomial infection, rapid detection, mecA gene

  19. The false sero-negativity of brucella standard agglutination test: Prozone phenomenon

    Directory of Open Access Journals (Sweden)

    İrfan Binici

    2011-12-01

    Full Text Available Objectives: We aimed to assess prozone phenomenon that is quite rare and causes false negativity in serological diagnosisof brucellosis with standard dilution titers.Materials and methods: In this study the tests of four cases that have false negative serological results were evaluated.Blood cultures were obtained from all cases while cerebrospinal fluid cultures were studied in the two cases. Standardagglutination test (SAT and Coombs test were performed to all patients.Results: SAT and Coombs test was negative in titers up to 1/640 in all cases. The SAT and Coombs tests in cerebrospinalfluid (CSF of the two cases with neurobrucellosis diagnosis were negative, as well. Since the clinical and laboratoryfindings suggested the brucellosis, the serums were restudied by diluting up to 1/10240 titer and we saw that the first3 cases became positive at a titer of 1/1280. The fourth case remained negative and therefore, we applied high dilutionCoombs test. This time the test gave a positive result at 1/10240 titer beginning from 1/2560 titer. B.melitensis wasisolated from two cases.Conclusion: SAT and Coombs’ test must be diluted to titers 1/2560 or more in order to exclude false sero-negativity incases with clinical and laboratory findings suggesting brucellosis. J Microbiol Infect Dis 2011; 1(3:110-113

  20. A latex agglutination test for the field determination of abnormal vitellogenin production in male fishes contaminated by estrogen mimics

    Energy Technology Data Exchange (ETDEWEB)

    Magalhaes, Ilizabete [Laboratoire d' Immunologie-Microbiologie (LIM/ESE-CNRS, unite FRE2635), IUT de Thionville-Yutz, 1, Impasse A Kastler, F-57970 Yutz (France); Pihan, Jean-Claude [Laboratoire de Production des Ecosystemes et Ecotoxicologie (LBFE), UFR SciFa, campus Bridoux, rue du G Delestraint, F-57070 Metz (France); Falla, Jairo [Laboratoire d' Immunologie-Microbiologie (LIM/ESE-CNRS, unite FRE2635), IUT de Thionville-Yutz, 1, Impasse A Kastler, F-57970 Yutz (France)

    2004-06-09

    Estrogen mimics are pollutants present in the aquatic environment. These compounds induce abnormalities in the reproductive system of male fishes, which lead to a total or partial male feminization, or to their demasculinization. Ultimately, these alterations could lead to a disappearance of the total contaminated fish population. Moreover, these toxic substances possess the capacity to mimic endogenous estrogens and to induce the abnormal production of vitellogenin (VTG) in male and immature fishes. The purpose of this research was to develop an easy, specific, cheap and fast method for diagnosing the contamination of male fishes by estrogen mimics, using VTG as biomarker. The selected method is based on a reverse latex agglutination test (rLAT), developed with monoclonal antibodies specific of this biomarker. The development of this VTG-rLAT has involved, firstly, the purification of carp VTG to produce monoclonal antibodies, specifics of this protein. One of these antibodies was selected to recover latex particles (diameter: 1 {mu}m). Finally, the immunoreactivity of the VTG-rLAT was verified with different fish plasma samples from males treated with 17{beta}-estradiol and non-treated males or females in vitellogenesis.

  1. THE PERSISTENCE OF LEPTOSPIRAL AGGLUTININS TITERS IN HUMAN SERA DIAGNOSED BY THE MICROSCOPIC AGGLUTINATION TEST

    Directory of Open Access Journals (Sweden)

    Eliete C. ROMERO

    1998-05-01

    Full Text Available The persistence of agglutinins detected by MAT has created some problems to the interpretation of the results. The aim of this study was to examine the data of serology from 70 patients with serologically confirmed diagnosis of leptospirosis by during 3-13 months after being affected with leptospires in order to elucidate the interpretation of the persistence of agglutinins detected by MAT. Sixty-one patients sera (87.14% had titers equal or greater than 800. Of these, two individuals maintained titers of 800 thirteen months after the onset. This study showed that only one sample of sera with high titers is not reliable to determine the time at which infection occurred.Persistência de títulos de aglutininas anti-leptospiras em soros humanos diagnosticados pelo teste de aglutinação microscópica A persistência de aglutininas detectadas por MAT tem criado problemas na interpretação dos resultados. O objetivo deste trabalho foi examinar os resultados da sorologia de 70 pacientes com confirmação sorológica de leptospirose durante 3-13 meses após terem sido infectados para se poder elucidar a interpretação da persistência de aglutininas detectadas por MAT. Sessenta e um soros de pacientes (87,14% apresentaram títulos iguais, ou maiores, que 800. Destes, 2 indivíduos mantiveram títulos de 800 treze meses após terem sido infectados. Este estudo mostra que apenas uma amostra de soro, mesmo com alto título de aglutininas, não pode ser considerada para determinar a fase da doença.

  2. The Classroom-Friendly ABO Blood Types Kit: Blood Agglutination Simulation

    Science.gov (United States)

    Arnold, Savittree Rochanasmita; Kruatong, Tussatrin; Dahsah, Chanyah; Suwanjinda, Duongdearn

    2012-01-01

    The classroom-friendly ABO blood type kit was developed by combining advantages of modelling and a simulation laboratory to teach the topics of ABO blood types and blood transfusion. Teachers can easily simulate the agglutination reaction on a blood type testing plate in the classroom, and show the students how this reaction occurs by using the…

  3. Traces of dissolved particles, including coccoliths, in the tests of agglutinated foraminifera from the Challenger Deep (10,897 m water depth, western equatorial Pacific)

    Science.gov (United States)

    Gooday, A. J.; Uematsu, K.; Kitazato, H.; Toyofuku, T.; Young, J. R.

    2010-02-01

    We examined four multilocular agglutinated foraminiferan tests from the Challenger Deep, the deepest point in the world's oceans and well below the depth at which biogenic and most detrital minerals disappear from the sediment. The specimens represent undescribed species. Three are trochamminaceans in which imprints and other traces of dissolved agglutinated particles are visible in the orange or yellowish organic test lining. In Trochamminacean sp. A, a delicate meshwork of organic cement forms ridges between the grain impressions. The remnants of test particles include organic structures identifiable as moulds of coccoliths produced by the genus Helicosphaera. Their random alignment suggests that they were agglutinated individually rather than as fragments of a coccosphere. Trochamminacean sp. C incorporates discoidal structures with a central hole; these probably represent the proximal sides of isolated distal shields of another coccolith species, possibly Hayaster perplexus. Imprints of planktonic foraminiferan test fragments are also present in both these trochamminaceans. In Trochamminacean sp. B, the test surface is densely pitted with deep, often angular imprints ranging from roughly equidimensional to rod-shaped. The surfaces are either smooth, or have prominent longitudinal striations, probably made by cleavage traces. We presume these imprints represent mineral grains of various types that subsequently dissolved. X-ray microanalyses reveal strong peaks for Ca associated with grain impressions and coccolith remains in Trochamminacean sp. C. Minor peaks for this element are associated with coccolith remains and planktonic foraminiferan imprints in Trochamminacean sp. A. These Ca peaks possibly originate from traces of calcite remaining on the test surfaces. Agglutinated particles, presumably clay minerals, survive only in the fourth specimen (' Textularia' sp.). Here, the final 4-5 chambers comprise a pavement of small, irregularly shaped grains with flat

  4. Evaluation of chromogenic medium and direct latex agglutination test for detection of group B streptococcus in vaginal specimens from pregnant women in Lebanon and Kuwait.

    Science.gov (United States)

    Ghaddar, Nahed; Alfouzan, Wadha; Anastasiadis, Elie; Al Jiser, Tamima; Itani, Saad Eddine; Dernaika, Racha; Eid, Toufic; Ghaddar, Ali; Charafeddine, Adib; Dhar, Rita; El Hajj, Hiba

    2014-10-01

    This study was undertaken to evaluate chromogenic medium and a direct latex agglutination test (DLA) for detection of Group B Streptococcus (GBS) in the vaginal specimens of pregnant women, and to ascertain the prevalence of GBS in this population in Kuwait and Lebanon. Vaginal swabs, collected from women at 35-37 weeks of gestation, were cultured on 5 % sheep blood agar (SBA), colistin nalidixic acid agar (CNA), Strept B Select chromogenic agar (SBS) as well as Lim enrichment broth in 168 cases in Lebanon while only SBA was used for 1391 samples in Kuwait. In addition, vaginal samples from 102 GBS-positive and 20 GBS-negative women near the time of delivery were collected in Kuwait for evaluation of the DLA test. During the study period, the prevalence of GBS colonization was determined to be 20.7 % (288/1391) in Kuwait while 18.4 % (31) of 168 pregnant women in Lebanon had vaginal cultures positive for GBS. By direct plating of vaginal swabs on the three media used, the isolation rates of GBS were 51.6, 64.5 and 77.4 % on SBA, CNA and SBS, respectively, which increased to 90.35, 93.1 and 96.8 %, respectively, following subculture in Lim broth after 18 h of incubation. The sensitivity of the DLA test was found to be dependent on the density of GBS colonization, resulting in 100 % sensitivity and 100 % specificity for heavy (>10(2) c.f.u. per swab) and moderately heavy (50-100 c.f.u. per swab) growth of GBS. However, for vaginal specimens yielding <50 c.f.u. per swab, the sensitivity, specificity, positive and negative predictive values of the DLA test were 100, 55.5, 63.6 and 100 %, respectively. In conclusion, a chromogenic agar, such as SBS, and a DLA test can be used for rapid detection of GBS in pregnant women. The DLA test, in particular, could prove to be a useful tool for immediate detection of GBS in women near delivery so that intrapartum antibiotic prophylaxis can be initiated.

  5. Application of Direct Agglutination Test (DAT for the Diagnosis and Seroepide-miological Studies of Visceral Leishmaniasis in Iran

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    S Charehdar

    2006-08-01

    Full Text Available Visceral leishmaniasis (VL is one of the most important parasitic diseases which is endemic in different parts of Iran. Serological studies were conducted by direct agglutination test (DAT on 12144 human serum samples, collected from four geographical zones of Iran. Sero prevalence, geographical distribution, clinical signs and symptoms for human visceral leishmaniasis based on DAT for the period of 2002 through 2005 were determined. From 516 kala-azar cases detected: 50.6% were from Meshkin-shahr and Moghan districts in Ardabil Province, northwest of Iran and 49.4% were detected from other areas of Iran. In physical examination of seropositive cases, which were detected by DAT with anti-leishmanial antibodies at titers of 1: 3200 to 1: 102400, almost 50% of suspected individuals showed the classical kala-azar signs and symptoms. Predominant signs and symptoms in 233 hospitalized patients with anti-Leishmania antibodies at 1:3200 and higher, were fever (88.0% and splenomegaly (84.5%. Statistically significant difference was found between males (58% and females (42% (P< 0.01. Moreover, 93.6% of the VL patients were < 5 yr of age, and 6.4% were older than 5 yr that this difference was statistically significant (P< 0.01. From 1383 serum samples collected from domestic dogs in the villages that are known as endemic foci of human leishmaniasis, 152 (11.0% were positive by DAT (≥ 1:320. Parasitological and serological examinations that were performed in 30 wild canines showed that 10% of these animals were infected by L. infantum. L. infantum Lon49 is the principal agent of the disease in human as well as animal reservoir hosts in different parts of Iran. For the first time in Iran, L. tropica isolated from both skin lesions in the face and bone marrow aspiration in a HIV+ man who co-infected with VL as well as in an infected dog from Ardabil Province.

  6. Rapid identification of Mycobacterium species by lectin agglutination.

    Science.gov (United States)

    Athamna, Abed; Cohen, Dani; Athamna, Muhammad; Ofek, Itzhak; Stavri, Henriette

    2006-05-01

    The purpose of the present study is to explore the possibility that plant lectins can be used for the development of rapid and inexpensive technique for differentiation of mycobacterial species. The method is based on interaction between mycobacteria and lectins as visualized by agglutination in a microtiter plate. We employed 18 mycobacterium species and determined the minimal lectin concentration (MLC) of 23 different lectins. For some of the bacteria as a high as 1000 microg/ml of one or more lectins were required to induce agglutination, while for other strains as low as 1.95 microg/ml of the lectin were needed. A unique pattern of agglutination was observed for each species over a range of 62-1000 microg/ml lectin concentrations. There were little or no variations in MLC within strains (intraspecies) of each of two species tested. In contrast, there were marked interspecies variations in MLC. Analysis of the MLC showed that the highest score of interspecies differences with 23 lectins was obtained at 125 microg/ml lectin concentration. At this concentration it was found that the pattern of agglutinations with only two lectins was sufficient to differentiate mycobacterium species from each other. Because the bacteria-lectin interaction is adaptable to various methods of visualization, our findings may set the stage for developing a rapid and reliable tool to differentiate mycobacterium species.

  7. Corrosion test cell for bipolar plates

    Science.gov (United States)

    Weisbrod, Kirk R.

    2002-01-01

    A corrosion test cell for evaluating corrosion resistance in fuel cell bipolar plates is described. The cell has a transparent or translucent cell body having a pair of identical cell body members that seal against opposite sides of a bipolar plate. The cell includes an anode chamber and an cathode chamber, each on opposite sides of the plate. Each chamber contains a pair of mesh platinum current collectors and a catalyst layer pressed between current collectors and the plate. Each chamber is filled with an electrolyte solution that is replenished with fluid from a much larger electrolyte reservoir. The cell includes gas inlets to each chamber for hydrogen gas and air. As the gases flow into a chamber, they pass along the platinum mesh, through the catalyst layer, and to the bipolar plate. The gas exits the chamber through passageways that provide fluid communication between the anode and cathode chambers and the reservoir, and exits the test cell through an exit port in the reservoir. The flow of gas into the cell produces a constant flow of fresh electrolyte into each chamber. Openings in each cell body is member allow electrodes to enter the cell body and contact the electrolyte in the reservoir therein. During operation, while hydrogen gas is passed into one chamber and air into the other chamber, the cell resistance is measured, which is used to evaluate the corrosion properties of the bipolar plate.

  8. An evaluation of the relative sensitivities of the venereal disease research laboratory test and the Treponema pallidum particle agglutination test among patients diagnosed with primary syphilis.

    Science.gov (United States)

    Creegan, Linda; Bauer, Heidi M; Samuel, Michael C; Klausner, Jeffrey; Liska, Sally; Bolan, Gail

    2007-12-01

    Because definitive methods for diagnosing primary syphilis are limited, it is important to optimize the sensitivity of serodiagnosis. To determine the most sensitive testing approach to the diagnosis of primary syphilis, using the commonly available serologic tests: the Venereal Disease Research Laboratory (VDRL) test and the Treponema pallidum particle agglutination (TP-PA) test. Sensitivities of 2 serologic testing strategies for primary syphilis were compared among 106 darkfield-confirmed cases treated in San Francisco from January 2002 through December 2004. The sensitivity of the diagnostic strategy using VDRL confirmed by TP-PA was 71% (95% CI, 61%-79%). Substituting Rapid Plasma Reagin test for VDRL in a subset of 51 patients produced the same sensitivity (71%; 95% CI, 56%-83%). The sensitivity of TP-PA as the first-line diagnostic test was 86% (95% CI, 78%-92%). The sensitivity of the former approach was significantly lower among HIV-positive patients, compared with HIV-negative patients (55% vs. 77%, P = 0.05). The TP-PA test as the first-line diagnostic test yielded higher sensitivity for primary syphilis than did the use of the currently recommended strategy.

  9. 改进试管凝集试验法检测布鲁氏菌病抗体%Detection of Anti-Brucella abortus Sera Agglutination Titers with Modified Tube Agglutination Test

    Institute of Scientific and Technical Information of China (English)

    李翠; 关孚时; 戴志红; 蒋卉; 温芳; 陆连寿; 王在时

    2012-01-01

    In order to prove the use value of the modified tube agglutination test,six anti-Brucella abortus sera were detected with MSAT and SAT referred to the international standard.The results showed that the sera titers were almost consistent detected with SAT and MSAT and the advantages of MSAT could be list as follows: The dilution method was simpler and more accurate.We could save time and effort,and save the materials such as serum,antigen etc.The result was easier to determine and with good reproducibility.%为证实改进的试管凝集试验(SAT)的使用价值,分别用微量试管凝集试验(MSAT)和SAT以国际标准血清为参比检测6份牛布鲁氏菌病阳性血清的抗体效价。结果表明:MSAT和SAT试验结果一致,而且MSAT具有稀释方法更简便、精确,省时省力,可节约血清、抗原等试验原材料,试验结果容易判定,重现性良好等优越性。

  10. Agglutination of Helicobacter pylori coccoids by lectins

    Institute of Scientific and Technical Information of China (English)

    Mar Mar Khin; Jie Song Hua; Hah Cong Ng; Bow Ho; Torkel Wadstrorr

    2000-01-01

    AIM To study the agglutination pattern of Helicobacter pylori coccoid and spiral forms.METHODS Assays of agglutination and agglutination inhibition were applied using fifteen commercial lectins. RESULTS Strong agglutination was observed with mannose-specific Concanavalin A (Con A ),fucose-specific Tetragonolobus purpureas ( Lotus A ) and N-acetyl glucosamine-specific Triticum vulgaris (WGA) lectins. Mannose and fucose specific lectins were reactive with all strains of H. pylori coccoids as compared to the spirals. Specific carbohydrates, glycoproteins and mucin were shown to inhibit H. pylori lectin-agglutination reactions. Pre-treatment of the bacterial cells with formalin and sulphuric acid did not alter the agglutination patterns with lectins. However, sodium periodate treatment of bacterial cells were shown to inhibit agglutination reaction with Con A, Lotus A and WGA lectins. On the contrary, enzymatic treatment of coccoids and spirals did not show marked inhibition of H. pylori-lectin agglutination. Interestingly, heating of H.pylori cells at 60℃ for 1 hour was shown to augment the agglutination with all of the lectins tested. CONCLUSION The considerable differences in lectin agglutination patterns seen among the two differentiated forms of H. pylori might be attributable to the structural changes during theevents of morphological transformation,resulting in exposing or masking some of the sugar residues on the cell surface. Possibility of various sugar residues on the cell wall of the coccoids may allow them to bind to different carbohydrate receptors on gastric mucus and epithelial cells. The coccoids with adherence characteristics like the spirals could aid in the pathogenic process of Helicobacter infection.This may probably lead to different clinical outcome of H. pylori associated gastroduodenal disease.

  11. DNA & Protein detection based on microbead agglutination

    KAUST Repository

    Kodzius, Rimantas

    2012-06-06

    We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microparticles in the presence of a specific analyte thus enabling the macroscopic observation. Agglutination-based tests are most often used to explore the antibody-antigen reactions. Agglutination has been used for mode protein assays using a biotin/streptavidin two-component system, as well as a hybridization based two-component assay; however, as our work shows, two-component systems are prone to self-termination of the linking analyte and thus have a lower sensitivity. Three component systems have also been used with DNA hybridization, as in our work; however, their assay requires 48 hours for incubation, while our assay is performed in 5 minutes making it a real candidate for POC testing. We demonstrate three assays: a two-component biotin/streptavidin assay, a three-component hybridization assay using single stranded DNA (ssDNA) molecules and a stepped three-component hybridization assay. The comparison of these three assays shows our simple stepped three-component agglutination assay to be rapid at room temperature and more sensitive than the two-component version by an order of magnitude. An agglutination assay was also performed in a PDMS microfluidic chip where agglutinated beads were trapped by filter columns for easy observation. We developed a rapid (5 minute) room temperature assay, which is based on microbead agglutination. Our three-component assay solves the linker self-termination issue allowing an order of magnitude increase in sensitivity over two–component assays. Our stepped version of the three-component assay solves the issue with probe site saturation thus enabling a wider range of detection. Detection of the agglutinated beads with the naked eye by trapping in microfluidic channels has been shown.

  12. Survey of False-positive Reactivity of Latex Agglutination Test for Kala-azar (Katex) without Urine Sample Boiling Process in Autoimmune Patients.

    Science.gov (United States)

    Ghatee, Mohammad Amin; Kanannejad, Zahra; Sharifi, Iraj; Askari, Asma; Bamorovat, Mehdi

    2017-06-01

    Latex agglutination test for Kala-azar (KAtex) is an easy, inexpensive, and field-applicable antigen detection test. However, the main drawback of this method is the boiling step applied to remove false positivity of the test. This study was conducted to survey false positivity results of latex agglutination test for KAtex without boiling process in urine of some autoimmune patients. Ninety-two urine samples from autoimmune patients including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), scleroderma, autoimmune vasculitis, vitiligo, pemphigus and Wagner cases and 20 urine samples from healthy individuals were collected from Kerman Province in Southeastern Iran in 2010-2011. All urine samples were checked by KAtex after boiling for 5 min false positivity rate of the test was surveyed in different healthy and patients groups while boiling process was removed. Rheumatoid factor (RF) then was checked in sera of all cases to evaluate the relationship between RF and KAtex false positivity. All samples represented negative results with KAtex when boiling was performed (100% specificity). Then, 20% positivity was evident in healthy cases. False-positive reactivity was more prominent observed in patient groups than healthy individuals, except in vitiligo. However, a significant difference was only observed in RA group (Pboiling process removal.

  13. Evaluation of Western blot, ELISA and latex agglutination tests to detect Toxoplasma gondii serum antibodies in farmed red deer.

    Science.gov (United States)

    Patel, Kandarp Khodidas; Howe, Laryssa; Heuer, Cord; Asher, Geoffery William; Wilson, Peter Raymond

    2017-09-15

    Abortion due to Toxoplasma gondii has been suspected in New Zealand farmed red deer. However, knowledge around the epidemiology and prevalence of T. gondii in farmed red deer is limited. The aim of this study was to firstly, assess the sensitivity and specificity of two commercially available assays, ELISA and latex agglutination test (LAT), for use in deer and secondly, to estimate the sero-prevalence of T. gondii in red deer. A total of 252 sera from rising 2-year-old and adult hinds from 17 New Zealand red deer herds at early and late pregnancy scanning and from known aborted and/or non-aborted hinds were tested for the presence of T. gondii antibodies. Each assays' sensitivity and specificity was evaluated by both the Western Blot (WB) as a gold standard method and Bayesian latent class (BLC) analysis in the absence of a gold standard. The sensitivity and specificity for WB were 95.8% (95% credible interval: 89.5-99.2%) and 95.1% (95% credible interval: 90.6-98.1%), respectively. For the LAT at the manufacturer's recommended ≥1:32 cut-off titre, the sensitivity (88.7%, 95% credible interval: 80.8-94.7%) and specificity (74.3%, 95% credible interval: 67.5-80.5%) were lower and higher than the sensitivity (76.2%, 95% credible interval: 66.7-84.5%) and specificity (89.7%, 95% credible interval: 84.5-93.9%) at a ≥1:64 cut-off, using (BLC) analysis. Sensitivity and specificity of the LAT at cut-off titre of 1:32 were estimated to be 84.4% (95% CI: 74.9-90.9%) and 73.5% (95% CI: 65.8-79.9%) against WB. The LAT had better agreement with WB at cut-off titre of ≥1:64 than ≥1:32 (Kappa=0.63 vs 0.54). At optimised cut-off S/P of 15.5%, the sensitivity (98.8%, 95% credible interval 96.1-99.8%) and specificity (92.8%, 95% credible interval 88.9-95.7%) of the ELISA were higher and lower, respectively, than the sensitivity (85.1%, 95% credible interval 76.2-91.9%) and specificity (98.5%, 95% credible interval 96.9-99.4%) at manufacturer's cut-off S/P of 30%, from BLC

  14. Evaluation of recombinant LigB antigen-based indirect ELISA and latex agglutination test for the serodiagnosis of bovine leptospirosis in India.

    Science.gov (United States)

    Deneke, Yosef; Sabarinath, T; Gogia, Neha; Lalsiamthara, Jonathan; Viswas, K N; Chaudhuri, Pallab

    2014-08-01

    Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the genus Leptospira, causing febrile infection characterized by multi-organ failure in humans and animals. Leptospiral Ig-like protein B (LigB) is a surface-expressed antigen that mediates host cell invasion or attachment. In this study, N-terminal conserved region of LigB protein (46 kDa) was evaluated for its diagnostic potential to detect anti-leptospiral antibodies in the sera of various animal species. Dot blot analysis revealed immunoreactivity of Leptospira-positive sera of cattle, buffalo, dog, sheep and goat to purified LigB protein. We have analyzed 1126 bovine serum samples, collected from Northern and Eastern part of India, by microscopic agglutination test (MAT) and recombinant LigB (rLigB) based ELISA and latex agglutination test (LAT). The sensitivity of rLigB based ELISA for 554 MAT positive sera was 96.9% and the specificity with 572 MAT negative sera was 91.08% whereas LAT showed sensitivity and specificity of 93.68% and 92.31%, respectively. Kappa values of 0.879 and 0.860 for recombinant antigen based ELISA and LAT indicate excellent agreement with the gold standard serological test, MAT, for the detection of anti-leptospiral antibodies in sera. Further, LAT based on rLigB antigen is a simple and rapid test, suitable for serodiagnosis of leptospirosis under field conditions, owing to its portability and longer shelf life.

  15. Comparison of indirect fluorescent antibody test and the modified agglutination test for the detection of Toxoplasma gondii antibodies in stray dogs from Southern Brazil.

    Science.gov (United States)

    de Almeida, Jonatas Campos; Frehse, Michelle Salmon; Navarro, Italmar Teodorico; Garcia, João Luis; Biondo, Alexander Welker; Freire, Roberta Lemos

    2016-12-01

    The aim of the present study was to determine the prevalence of antibodies to Toxoplasma gondii by two serological techniques in sera of 364 stray dogs from Brazil by immunofluorescence antibody test (IFAT, cut off point 1:16) and to the modified agglutination test (MAT, cut-off points 1:25 and 1:50). A total of 175/364 (48.07%) sera were positive by IFAT, and 108/364 (29.67%) and 85/364 (23.35%) were positive by MAT with cutoff points 1:25 and 1:50, respectively were positive by MAT. Cohen's Kappa Coefficient between IFAT and MAT was 0.81 (excellent) and 0.66 (substantial) with cutoff points 1:25 and 1:50, respectively. Using IFAT as gold standard, MAT sensitivity and specificity were 78% and 99% for 1:25 and 61% and 99% for 1:50, respectively. The results document of the usefulness of MAT for serological diagnosis because it does not require species-specific conjugate.

  16. COMPARISON STUDY BETWEEN IN-HOUSE IGM DOT-ELISA AND THE MICROSCOPIC AGGLUTINATION TEST (MAT FOR THE DIAGNOSIS OF HUMAN LEPTOSPIROSIS

    Directory of Open Access Journals (Sweden)

    Ganesh Kumar A

    2012-04-01

    Full Text Available An indirect enzyme-linked immunosorbent assay (ELISA was compared with the microscopic agglutination test (MAT for the diagnosis of bovine leptospirosis. Blood samples from a total number of 319 HBsAg negative suspected leptospirosis case’s were received from Government Hospital and from a few private hospitals of Salem district, Tamilnadu, India. The serum samples were examined for the presence of anti leptospiral antibodies using a commercial qualitative method of an in-house Dot-ELISA assay and the results were compared with WHO standard Microscopic Agglutination Test (MAT. The following interesting results were noted, 132 (41.7 % serum samples were positive to Dot-ELISA, while 130 (40.7 % were positive to MAT. All samples positive to MAT were positive to Dot-ELISA, on of the samples were positive for MAT and negative to Dot-ELISA. The Dot-ELISA showed 100% sensitivity compared to MAT. The current diagnostic Dot-ELISA appears as a rapid, non hazardous and better alternative to MAT for the diagnosis of human Leptospirosis.

  17. Orion Boiler Plate Airdrop Test System

    Science.gov (United States)

    Machin, Ricardo A.; Evans, Carol T.

    2013-01-01

    On the 29th of February 2012 the Orion Capsule Parachute Assembly System (CPAS) project attempted to perform an airdrop test of a boilerplate test article for the second time. The first attempt (Cluster Development Test 2, July 2008) to deliver a similar boilerplate from a C-17 using the Low Velocity Air Drop (LVAD) technique resulted in the programmer parachute failing to properly inflate, the test article failing to achieve the desired test initiation conditions, and the test article a total loss. This paper will pick up where the CDT-2 failure investigation left off, describing the test technique that was adopted, and outline the modeling that was performed to gain confidence that the second attempt would be successful. The second boiler plate test (Cluster Development Test 3-3) was indeed a complete success and has subsequently been repeated several times, allowing the CPAS project to proceed with the full scale system level development testing required to integrate the hardware to the first Entry Flight Test vehicle as well as go into the Critical Design Review with minimum risk and a mature design.

  18. [Acute bacterial meningitis with soluble antigen detected by latex particle agglutination tests at the Sourô-Sanou University Hospital of Bobo-Dioulasso (Burkina Faso)].

    Science.gov (United States)

    Ouédraogo, S M; Yaméogo, T M; Kyelem, C G; Poda, G E A; Ouédraogo, N F; Millogo, A; Ouédraogo, A; Ouédraogo-Traoré, A; Drabo, Y J

    2012-01-01

    Acute bacterial meningitis constitutes a major public health problem in Burkina Faso, in part because of its high lethality rate, estimated in 2004 at 17.5%. Failure to confirm suspected cases of meningitis results in overestimating reported cases and incorrectly treating false positives. The latex particle agglutination test is a diagnostic alternative that overcomes these limitations. Determine the bacteriological and therapeutic profile as well as the course of cases of acute meningitis confirmed by the latex agglutination test at Sourô-Sanou University Hospital. This prospective longitudinal study took place over a one-year period (2008 to 2009). Data were collected from clinical and laboratory records. The diagnosis of meningitis was confirmed by testing for specific soluble antigens in the spinal fluid. We used the Pastorex(™) Meningitis Kit for that purpose. The threshold of significance selected for our study was 0.05. In all, 457 samples of spinal fluid from patients with suspected acute bacterial meningitis were analyzed and the latex test was performed in 438 of these samples: 154 (35.2%) were positive. The average age of our cases confirmed by the latex test was 13.2 ± 4.2 years old. This test confirmed more cases than any other method of identification. The therapeutic strategy used from one to four treatment agents. Streptococcus pneumoniae was the most virulent and the most lethal pathogen, with a 64.7% lethality rate. The earliness of the consultation and the treatment of the bacterial meningitis seem to have a positive effect on the course of disease.

  19. Sero-epidemiology of equine toxoplasmosis using a latex agglutination test in the three metropolises of Punjab, Pakistan.

    Science.gov (United States)

    Saqib, M; Hussain, M H; Sajid, M S; Mansoor, M K; Asi, M N; Fadya, A A K; Zohaib, A; Sial, A U R; Muhammad, G; Ullah, I

    2015-06-01

    Toxoplasmosis is a serious threat for livestock in addition to being of zoonotic significance. In this study, serodiagnosis of equine toxoplasmosis was conducted in a randomly selected population from the 3 metropolises of Punjab, Pakistan. To this end, 272 draught equines were screened using a commercial latex agglutination assay kit. Association of probable risk factors of equine toxoplasmosis was also documented. A total of 91 (33.5%) equines were found sero-positive for Toxoplama (T.) gondii having antibody titers ranging between 1:32 to 1:612. The highest rates of seropositive cases were observed in donkeys (58.7%) followed by mules (28.6%) and horses (23.5%). Age, sex and species of draught equines were found not to be statistically (p>0.05) associated with the distribution of T. gondii antibodies. The results of the study provided a baseline data for the exposure of equine population in this area. In addition, it is recommended that the contiguous population of domestic ruminants and possible reservoirs such as feral cats should be screened in order to explore the potential risk for the human population in Pakistan.

  20. Usefulness of the rK39-Immunochromatographic Test, Direct Agglutination Test, and Leishmanin Skin Test for Detecting Asymptomatic Leishmania Infection in Children in a New Visceral Leishmaniasis Focus in Amhara State, Ethiopia

    Science.gov (United States)

    Gadisa, Endalamaw; Custodio, Estefanía; Cañavate, Carmen; Sordo, Luis; Abebe, Zelalem; Nieto, Javier; Chicharro, Carmen; Aseffa, Abraham; Yamuah, Lawrence; Engers, Howard; Moreno, Javier; Cruz, Israel

    2012-01-01

    In areas where visceral leishmaniasis is anthroponotic, asymptomatically infected patients may play a role in transmission. Additionally, the number of asymptomatic patients in a disease-endemic area will also provide information on transmission dynamics. Libo Kemkem and Fogera districts (Amhara State, Ethiopia) are now considered newly established areas to which visceral leishmaniasis is endemic. In selected villages in these districts, we conducted a study to assess the usefulness of different approaches to estimate the asymptomatic infection rate. Of 605 participants, the rK39 immunochromatographic test was able to detect asymptomatic infection in 1.5% (9 of 605), direct agglutination test in 5.3% (32 of 605), and leishmanin skin test in 5.6% (33 of 589); the combined use of serologic methods and leishmanin skin test enabled detecting asymptomatic infection in 10.1% (61 of 605). We conclude that the best option to detect asymptomatic infection in this new visceral leishmaniasis–endemic focus is the combined use of the direct agglutination test and the leishmanin skin test. PMID:22556076

  1. Usefulness of the rK39-immunochromatographic test, direct agglutination test, and leishmanin skin test for detecting asymptomatic Leishmania infection in children in a new visceral leishmaniasis focus in Amhara State, Ethiopia.

    Science.gov (United States)

    Gadisa, Endalamaw; Custodio, Estefanía; Cañavate, Carmen; Sordo, Luis; Abebe, Zelalem; Nieto, Javier; Chicharro, Carmen; Aseffa, Abraham; Yamuah, Lawrence; Engers, Howard; Moreno, Javier; Cruz, Israel

    2012-05-01

    In areas where visceral leishmaniasis is anthroponotic, asymptomatically infected patients may play a role in transmission. Additionally, the number of asymptomatic patients in a disease-endemic area will also provide information on transmission dynamics. Libo Kemkem and Fogera districts (Amhara State, Ethiopia) are now considered newly established areas to which visceral leishmaniasis is endemic. In selected villages in these districts, we conducted a study to assess the usefulness of different approaches to estimate the asymptomatic infection rate. Of 605 participants, the rK39 immunochromatographic test was able to detect asymptomatic infection in 1.5% (9 of 605), direct agglutination test in 5.3% (32 of 605), and leishmanin skin test in 5.6% (33 of 589); the combined use of serologic methods and leishmanin skin test enabled detecting asymptomatic infection in 10.1% (61 of 605). We conclude that the best option to detect asymptomatic infection in this new visceral leishmaniasis-endemic focus is the combined use of the direct agglutination test and the leishmanin skin test.

  2. Comparison of the H7 latex agglutination test with a fliCh7 real-time PCR assay for confirmation of the H type of Escherichia coli O157:H7

    Science.gov (United States)

    Escherichia coli O157:H7 is a food-borne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. Positive identification of E. coli O157:H7 is made using biochemical tests and latex agglutination using specific antisera. However, under certain conditions, some E. coli O157:H7 isolate...

  3. Validation of the modified agglutination test for the detection of Toxoplasma gondii in free-range chickens by using cat and mouse bioassay.

    Science.gov (United States)

    Dubey, J P; Laurin, E; Kwowk, O C H

    2016-03-01

    The modified agglutination test (MAT) is one of the most commonly used tests for the detection of antibodies to Toxoplasma gondii in animal and human sera. The objective of the present study was to evaluate the diagnostic accuracy of the MAT and bioassay in free-range/backyard (FR) chickens (Gallus domesticus). Previously-published T. gondii test results from 2066 chickens from 19 countries were compiled for the present study. The frequency of isolation of T. gondii increased for MAT titres between 1:5 and 1:160, and ranged from 61 to 75% for antibody titres of 1:160, 1:320, and ⩾1:640. Twenty-three cats fed pooled hearts from a total of 802 FR seronegative (MAT, <1:5) chickens from several countries did not excrete oocysts, indicating a high negative predictive value of MAT because FR chickens would have been exposed to many microbes; cats are the most sensitive indicators of T. gondii infection in tissues and can excrete millions of oocysts after ingesting even a few bradyzoites. Of the 29 cats in this study, six cats, fed hearts pooled from 15-122 FR chickens, excreted oocysts; but these identifications were likely related to misidentification or prozone. Results of the present study support the validity of MAT for the detection of T. gondii infection in chickens.

  4. Evaluation of the Widal tube agglutination test for the diagnosis of typhoid fever among children admitted to a rural hdospital in Tanzania and a comparison with previous studies

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    Malahiyo Rajabu

    2010-06-01

    Full Text Available Abstract Background The diagnosis of typhoid fever is confirmed by culture of Salmonella enterica serotype Typhi (S. typhi. However, a more rapid, simpler, and cheaper diagnostic method would be very useful especially in developing countries. The Widal test is widely used in Africa but little information exists about its reliability. Methods We assessed the performance of the Widal tube agglutination test among febrile hospitalized Tanzanian children. We calculated the sensitivity, specificity, positive predictive value (PPV, and negative predictive value (NPV of various anti-TH and -TO titers using culture-confirmed typhoid fever cases as the "true positives" and all other febrile children with blood culture negative for S. typhi as the "true negatives." Results We found that 16 (1% of 1,680 children had culture-proven typhoid fever. A single anti-TH titer of 1:80 and higher was the optimal indicator of typhoid fever. This had a sensitivity of 75%, specificity of 98%, NPV of 100%, but PPV was only 26%. We compared our main findings with those from previous studies. Conclusion Among febrile hospitalized Tanzanian children with a low prevalence of typhoid fever, a Widal titer of ≥ 1:80 performed well in terms of sensitivity, specificity, and NPV. However a test with improved PPV that is similarly easy to apply and cost-efficient is desirable.

  5. Tests of a Novel Design of Resistive Plate Chambers

    CERN Document Server

    Bilki, B; Freund, B; Neubüser, C; Onel, Y; Repond, J; Schlereth, J; Xia, L

    2015-01-01

    A novel design of Resistive Plate Chambers (RPCs), using only a single resistive plate, is being proposed. Based on this design, two large size prototype chambers were constructed and were tested with cosmic rays and in particle beams. The tests confirmed the viability of this new approach. In addition to showing an improved single-particle response compared to the traditional 2-plate design, the novel chambers also prove to be suitable for calorimetric applications.

  6. Development of a rapid agglutination latex test for diagnosis of enteropathogenic and enterohemorrhagic Escherichia coli infection in developing world: defining the biomarker, antibody and method.

    Directory of Open Access Journals (Sweden)

    Letícia B Rocha

    2014-09-01

    Full Text Available Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC/EHEC are human intestinal pathogens responsible for diarrhea in both developing and industrialized countries. In research laboratories, EPEC and EHEC are defined on the basis of their pathogenic features; nevertheless, their identification in routine laboratories is expensive and laborious. Therefore, the aim of the present work was to develop a rapid and simple assay for EPEC/EHEC detection. Accordingly, the EPEC/EHEC-secreted proteins EspA and EspB were chosen as target antigens.First, we investigated the ideal conditions for EspA/EspB production/secretion by ELISA in a collection of EPEC/EHEC strains after cultivating bacterial isolates in Dulbecco's modified Eagle's medium (DMEM or DMEM containing 1% tryptone or HEp-2 cells-preconditioned DMEM, employing either anti-EspA/anti-EspB polyclonal or monoclonal antibodies developed and characterized herein. Subsequently, a rapid agglutination latex test (RALT was developed and tested with the same collection of bacterial isolates.EspB was defined as a biomarker and its corresponding monoclonal antibody as the tool for EPEC/EHEC diagnosis; the production of EspB was better in DMEM medium. RALT assay has the sensitivity and specificity required for high-impact diagnosis of neglected diseases in the developing world.RALT assay described herein can be considered an alternative assay for diarrhea diagnosis in low-income countries since it achieved 97% sensitivity, 98% specificity and 97% efficiency.

  7. Direct appraisal of latex agglutination testing, a convenient alternative to enzyme immunoassay for the detection of rotavirus in childhood gastroenteritis, by comparison of two enzyme immunoassays and two latex tests.

    Science.gov (United States)

    Sambourg, M; Goudeau, A; Courant, C; Pinon, G; Denis, F

    1985-04-01

    During February and March 1984, 207 fecal samples from infants and children with gastroenteritis were tested for rotavirus with four techniques: two enzyme immunoassays (Rotazyme; Abbott Laboratories, North Chicago, Ill., and Enzygnost-Rotavirus; Calbiochem-Behring, La Jolla, Calif.) and two latex agglutination tests (Rotalex; Orion Research, Inc., Cambridge, Mass., and Slidex Rota-Kit; Biomérieux). All stool samples were also tested for yeasts and bacterial pathogens. Electron microscopy was used to investigate discrepant results. We found 47% positive samples with Enzygnost-Rotavirus, 38% with Rotazyme, 37% with Slidex Rota-Kit, and 34% with Rotalex. No specimen was found positive by Rotazyme only or Slidex Rota-Kit only. On the contrary, 12 samples which were positive with Enzygnost-Rotavirus only and 3 which were positive with Rotalex only were not confirmed as positive by electron microscopy. Both enzyme immunoassays gave 6% equivocal results; Slidex Rota-Kit gave significantly fewer equivocal results than did Rotalex: 2.9% versus 9.7% (P less than 0.01). The sensitivity and specificity of latex tests compared favorably with that of enzyme immunoassays. Latex agglutination tests can be performed by unskilled personnel and are rapid and relatively cheap. They appear to be very suitable for routine laboratory work and may prove useful for large-scale screening in developing countries.

  8. Quantitative tests for plate tectonics on Venus

    Science.gov (United States)

    Kaula, W. M.; Phillips, R. J.

    1981-01-01

    Quantitative comparisons are made between the characteristics of plate tectonics on the earth and those which are possible on Venus. Considerations of the factors influencing rise height and relating the decrease in rise height to plate velocity indicate that the rate of topographic dropoff from spreading centers should be about half that on earth due to greater rock-fluid density contrast and lower temperature differential between the surface and interior. Statistical analyses of Pioneer Venus radar altimetry data and global earth elevation data is used to identify 21,000 km of ridge on Venus and 33,000 km on earth, and reveal Venus ridges to have a less well-defined mode in crest heights and a greater concavity than earth ridges. Comparison of the Venus results with the spreading rates and associated heat flow on earth reveals plate creation rates on Venus to be 0.7 sq km/year or less and indicates that not more than 15% of Venus's energy is delivered to the surface by plate tectonics, in contrast to values of 2.9 sq km a year and 70% for earth.

  9. Development of a Rapid Agglutination Latex Test for Diagnosis of Enteropathogenic and Enterohemorrhagic Escherichia coli Infection in Developing World: Defining the Biomarker, Antibody and Method

    Science.gov (United States)

    Munhoz, Danielle D.; Cardoso, Lucas T. A.; Luz, Daniela E.; Andrade, Fernanda B.; Horton, Denise S. P. Q.; Elias, Waldir P.; Piazza, Roxane M. F.

    2014-01-01

    Background Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC/EHEC) are human intestinal pathogens responsible for diarrhea in both developing and industrialized countries. In research laboratories, EPEC and EHEC are defined on the basis of their pathogenic features; nevertheless, their identification in routine laboratories is expensive and laborious. Therefore, the aim of the present work was to develop a rapid and simple assay for EPEC/EHEC detection. Accordingly, the EPEC/EHEC-secreted proteins EspA and EspB were chosen as target antigens. Methodology First, we investigated the ideal conditions for EspA/EspB production/secretion by ELISA in a collection of EPEC/EHEC strains after cultivating bacterial isolates in Dulbecco’s modified Eagle’s medium (DMEM) or DMEM containing 1% tryptone or HEp-2 cells-preconditioned DMEM, employing either anti-EspA/anti-EspB polyclonal or monoclonal antibodies developed and characterized herein. Subsequently, a rapid agglutination latex test (RALT) was developed and tested with the same collection of bacterial isolates. Principal findings EspB was defined as a biomarker and its corresponding monoclonal antibody as the tool for EPEC/EHEC diagnosis; the production of EspB was better in DMEM medium. RALT assay has the sensitivity and specificity required for high-impact diagnosis of neglected diseases in the developing world. Conclusion RALT assay described herein can be considered an alternative assay for diarrhea diagnosis in low-income countries since it achieved 97% sensitivity, 98% specificity and 97% efficiency. PMID:25254981

  10. Stability of Freeze-Dried Sera Stored at Different Temperatures for the Detection of Anti-Leishmania infantum Antibodies Using Direct Agglutination Test.

    Directory of Open Access Journals (Sweden)

    Zahra Kakooei

    2014-11-01

    Full Text Available This study aimed to evaluate freeze-dried sera as an alternative to non-freeze dried for detection of anti-Leishmania infantum antibodies over the course of 11 months using the direct agglutination test (DAT.Altogether, 60 serum samples (30 from humans and 30 from dogs were collected from various geographical locations in Iran. All the collected sera were pooled and each pooled serum sample contained 10 different sera. In the beginning, the human and dog pooled sera were categorized as positive (weak and strong and negative based on anti-L. infantum antibodies using the DAT. All the freeze-dried and non-freeze-dried sera were stored at -70°C, -20°C, 4°C, 22-28°C and 56°C for 11 months. The positive and negative human and dog pooled sera were separately tested using the DAT each month and the results were compared to non-freeze-dried sera kept under the same conditions.We found strong agreement (100% between the results obtained from freeze-dried human and dog in strong DAT positive sera kept at -70°C, -20°C, 4°C and 22-28°C during this study. The human and dog pooled sera stored at 56°C were corrupted after 2 weeks. The DAT results were highly reproducible using freeze-dried human pooled sera in the beginning and month 11 of this study (CV = 0.036.Freeze-dried human and dog strong DAT positive sera are highly stable under different temperature conditions, are easy to transport and are safe for use as positive and negative serum controls in laboratories.

  11. C6 plate puncture testing report.

    Energy Technology Data Exchange (ETDEWEB)

    Vangoethem, Douglas J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Cordova, Theresa Elena [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Reu, Phillip L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2013-04-01

    There are numerous scenarios where critical systems could be subject to penetration by projectiles or fixed objects (e.g., collision, natural disaster, act of terrorism, etc.). It is desired to use computational models to examine these scenarios and make risk-informed decisions; however, modeling of material failure is an active area of research, and new models must be validated with experimental data. The purpose of this report is to document the experimental work performed from FY07 through FY08 on the Campaign Six Plate Puncture project. The goal of this project was to acquire experimental data on the puncture and penetration of metal plates for use in model validation. Of particular interest is the PLH failure model also known as the multilinear line segment model. A significant amount of data that will be useful for the verification and validation of computational models of ductile failure were collected during this project were collected and documented herein; however, much more work remains to be performed, collecting additional experimental data that will further the task of model verification.

  12. A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays.

    Science.gov (United States)

    Gu, Yi; Wang, Zedong; Cai, Yufeng; Li, Xiaoxing; Wei, Feng; Shang, Limin; Li, Jiping; Liu, Quan

    2015-09-01

    Toxoplasma gondii can infect almost all warm-blooded animals, and many serological methods have been developed to detect T. gondii infection in a variety of animal species. In the present study, the seroprevalence of T. gondii infection in farmed mink in northeast China was determined using the modified agglutination test (MAT), a Western blot (WB), and 3 enzyme-linked immunosorbent assays (ELISAs) with protein A/G conjugate, using either of 2 recombinant dense granule antigens, GRA1 and GRA7, or Toxoplasma soluble antigens (TSA). There was no significant difference between the detection results of the GRA1-, GRA7-, and TSA-ELISAs and WB (McNemar chi-square, P > 0.05), but a significant difference was observed between MAT and WB (P < 0.05). A near perfect agreement (97.0%) was found between the GRA7-ELISA and WB (κ = 0.83), and a substantial agreement (92.4-93.1%) was observed in the TSA- and GRA1-ELISAs (κ = 0.68-0.73). The GRA7-ELISA showed the highest sensitivity and specificity, and the lowest false-positive and negative rates, while the MAT gave both a low sensitivity and frequent false positives in comparison to the WB. Receiver operating characteristic analysis revealed the largest area under curve of 0.85 (95% confidence interval: 0.74-0.96), and the highest relative sensitivity (72.7%) and specificity (99.0%) for a cutoff value of 0.19 in the GRA7-ELISA. These results indicate that the GRA7-ELISA is suitable for detection of T. gondii infection in mink and that MAT should be used with caution.

  13. Evaluation of Two rK39 Dipstick Tests, Direct Agglutination Test, and Indirect Fluorescent Antibody Test for Diagnosis of Visceral Leishmaniasis in a New Epidemic Site in Highland Ethiopia

    Science.gov (United States)

    Cañavate, Carmen; Herrero, Merce; Nieto, Javier; Cruz, Israel; Chicharro, Carmen; Aparicio, Pilar; Mulugeta, Abate; Argaw, Daniel; Blackstock, Anna J.; Alvar, Jorge; Bern, Caryn

    2011-01-01

    We assessed the performance characteristics of two rK39 immunochromatographic tests, a direct agglutination test (DAT), and an indirect immunofluorescent antibody test (IFAT) in the site of a new epidemic of visceral leishmaniasis (VL) in northwestern Ethiopia. The study population was composed of 179 patients with suspected VL and 67 controls. The sensitivities of Kalazar Detect®, DiaMed-IT Leish®, DAT, and IFAT in 35 polymerase chain reaction–confirmed VL cases were 94.3%, 91.4%, 91.4%, and 100%, respectively, and the specificities were 98.5%, 94%, 98.5%, and 98.5%, respectively. In a Bayesian latent class analysis of all 246 specimens, the estimated sensitivities were 90.5%, 89%, 88.8%, and 96% for Kalazar Detect®, DiaMed-IT Leish®, DAT, and IFAT, respectively; DAT showed the highest estimated specificity (97.4%). Both rK39 immunochromatographic tests perform as well as DAT, and are suitable for VL diagnosis in first-level health centers in this area of Ethiopia. PMID:21212210

  14. Evaluation of two rK39 dipstick tests, direct agglutination test, and indirect fluorescent antibody test for diagnosis of visceral leishmaniasis in a new epidemic site in highland Ethiopia.

    Science.gov (United States)

    Cañavate, Carmen; Herrero, Merce; Nieto, Javier; Cruz, Israel; Chicharro, Carmen; Aparicio, Pilar; Mulugeta, Abate; Argaw, Daniel; Blackstock, Anna J; Alvar, Jorge; Bern, Caryn

    2011-01-01

    We assessed the performance characteristics of two rK39 immunochromatographic tests, a direct agglutination test (DAT), and an indirect immunofluorescent antibody test (IFAT) in the site of a new epidemic of visceral leishmaniasis (VL) in northwestern Ethiopia. The study population was composed of 179 patients with suspected VL and 67 controls. The sensitivities of Kalazar Detect(®), DiaMed-IT Leish(®), DAT, and IFAT in 35 polymerase chain reaction-confirmed VL cases were 94.3%, 91.4%, 91.4%, and 100%, respectively, and the specificities were 98.5%, 94%, 98.5%, and 98.5%, respectively. In a Bayesian latent class analysis of all 246 specimens, the estimated sensitivities were 90.5%, 89%, 88.8%, and 96% for Kalazar Detect(®), DiaMed-IT Leish(®), DAT, and IFAT, respectively; DAT showed the highest estimated specificity (97.4%). Both rK39 immunochromatographic tests perform as well as DAT, and are suitable for VL diagnosis in first-level health centers in this area of Ethiopia.

  15. Comparison of latex agglutination and co-agglutination for the diagnosis and prognosis of cryptococcal meningitis

    Directory of Open Access Journals (Sweden)

    Khyriem A

    2003-01-01

    Full Text Available PURPOSE: To compare a commercially available Latex agglutination test and an in house co-agglutination test for the detection of cryptococcal antigen in cases of chronic meningitis. METHODS: One hundred and fifty cerebrospinal fluid (CSF samples from 150 cases of chronic meningitis were tested for the presence of Cryptococcus neoformans by modified India ink, culture and antigen detection by latex agglutination test (LAT and co-agglutination (Co-A test. RESULTS: Thirty-nine cases were positive by one or more tests employed. Antigen detection in CSF by LAT and Co-A was found to be most sensitive (94.9% while culture was the least (25.6%. Of the two antigen detection methods, Co-A was found to be more sensitive than the LAT, the difference being statistically significant. Initial CSF antigen titres did not have any prognostic significance. CONCLUSIONS: Co-A for antigen detection is an inexpensive and useful adjunct to direct microscopy and culture for the diagnosis of cryptococcal meningitis, though its usefulness in prognosis needs to be evaluated further.

  16. Comparison of Buffered, Acidified Plate Antigen to Standard Serologic Tests for the Detection of Serum Antibodies to Brucella abortus in Elk (Cervus canadensis).

    Science.gov (United States)

    Clarke, P Ryan; Edwards, William H; Hennager, Steven G; Block, Jean F; Yates, Angela M; Ebel, Eric; Knopp, Douglas J; Fuentes-Sanchez, Antonio; Jennings-Gaines, Jessica; Kientz, Rebecca L; Simunich, Marilyn

    2015-07-01

    Brucellosis (caused by the bacterium Brucella abortus) is a zoonotic disease endemic in wild elk (Cervus canadensis) of the Greater Yellowstone Ecosystem, US. Because livestock and humans working with elk or livestock are at risk, validated tests to detect the B. abortus antibody in elk are needed. Using the κ-statistic, we evaluated the buffered, acidified plate antigen (BAPA) assay for agreement with the results of the four serologic tests (card test [card], complement fixation test [CF], rivanol precipitation plate agglutination test [RIV], standard plate agglutination test [SPT]) that are approved by the US Department of Agriculture for the detection of the B. abortus antibody in elk. From 2006 to 2010, serum samples collected from elk within B. abortus-endemic areas (n = 604) and nonendemic areas (n = 707) and from elk culture-positive for B. abortus (n = 36) were split and blind tested by four elk serum diagnostic laboratories. κ-Values showed a high degree of agreement for the card (0.876), RIV (0.84), and CF (0.774) test pairings and moderate agreement for the SPT (0.578). Sensitivities for the BAPA, card, RIV, CF, and SPT were 0.859, 0.839, 0.899, 1.00, and 0.813, whereas specificities were 0.986, 0.993, 0.986, 0.98, and 0.968, respectively. The positive predictive values and the negative predictive values were calculated for 2.6%, 8.8%, and 16.2% prevalence levels. These findings suggest the BAPA test is a suitable screening test for the B. abortus antibodies in elk.

  17. Bayesian estimation of true prevalence, sensitivity and specificity of indirect ELISA, Rose Bengal Test and Slow Agglutination Test for the diagnosis of brucellosis in sheep and goats in Bangladesh.

    Science.gov (United States)

    Rahman, A K M Anisur; Saegerman, Claude; Berkvens, Dirk; Fretin, David; Gani, Md Osman; Ershaduzzaman, Md; Ahmed, Muzahed Uddin; Emmanuel, Abatih

    2013-06-01

    The true prevalence of brucellosis and diagnostic test characteristics of three conditionally dependent serological tests were estimated using the Bayesian approach in goats and sheep populations of Bangladesh. Serum samples from a random selection of 636 goats and 1044 sheep were tested in parallel by indirect ELISA (iELISA), Rose Bengal Test (RBT) and Slow Agglutination Test (SAT). The true prevalence of brucellosis in goats and sheep were estimated as 1% (95% credibility interval (CrI): 0.7-1.8) and 1.2% (95% CrI: 0.6-2.2) respectively. The sensitivity of iELISA was 92.9% in goats and 92.0% in sheep with corresponding specificities of 96.5% and 99.5% respectively. The sensitivity and specificity estimates of RBT were 80.2% and 99.6% in goats and 82.8% and 98.3% in sheep. The sensitivity and specificity of SAT were 57.1% and 99.3% in goats and 72.0% and 98.6% in sheep. In this study, three conditionally dependent serological tests for the diagnosis of small ruminant brucellosis in Bangladesh were validated. Considerable conditional dependence between IELISA and RBT and between RBT and SAT was observed among sheep. The influence of the priors on the model fit and estimated parameter values was checked using sensitivity analysis. In multiple test validation, conditional dependence should not be ignored when the tests are in fact conditionally dependent.

  18. Spelling Correction in Agglutinative Languages

    CERN Document Server

    Oflazer, K

    1994-01-01

    This paper presents an approach to spelling correction in agglutinative languages that is based on two-level morphology and a dynamic programming based search algorithm. Spelling correction in agglutinative languages is significantly different than in languages like English. The concept of a word in such languages is much wider that the entries found in a dictionary, owing to {}~productive word formation by derivational and inflectional affixations. After an overview of certain issues and relevant mathematical preliminaries, we formally present the problem and our solution. We then present results from our experiments with spelling correction in Turkish, a Ural--Altaic agglutinative language. Our results indicate that we can find the intended correct word in 95\\% of the cases and offer it as the first candidate in 74\\% of the cases, when the edit distance is 1.

  19. 比浊法血小板聚集试验的影响因素研究%Analysis of influencing factors of turbidimetry for platelet agglutination test

    Institute of Scientific and Technical Information of China (English)

    石红婷; 周伯荣; 王融; 邓燕华; 关海涛; 刘子凡

    2012-01-01

    目的 在不同的实验条件下,观察影响比浊法血小板聚集试验的因素.方法 选择30例健康对照组和204例病例组,观察不同的血小板计数及诱导剂浓度对糖尿病患者餐前与餐后、采血后的检测时间、诱导剂的种类互补等不同试验条件下的血小板聚集率.结果 随着血小板计数的减少或增加,血小板聚集率相应的减少或增加;二磷酸腺苷(ADP)、胶原(COL)、花生四烯酸(AA)浓度增加,血小板最大聚集率增大,相应的阿司匹林抵抗(AR)或氯吡格雷抵抗(CR)的检出率增高;服用不同的抗血小板药物,糖尿病患者对以ADP、COL、AA为诱导剂测定的血小板聚集率,其影响不同;未空腹及检测时间超过3 h重复性较差;服药2周后,COL、AA诱导的血小板聚集率下降不明的患者,随着时间的延长,6个月后易重新出现CR.结论 血小板计数、诱导剂浓度、糖尿病疾病、空腹状态、检测时间与比浊法检测血小板聚集率相关,以ADP、COL、AA作诱导剂检测血小板聚集率较单一的ADP更全面、准确.%Objective To investigate the influencing factors of turbidimetry for platelet agglutination test under different experi -mental conditions .Methods 30 cases of healthy subjects and 204 cases of patents were enrolled . The rate of platelet aggregation (RPA ) was detected under different conditions , including platelet counts ,inducer concentration and types , diabetes mellitus (DM ), fasting state and detection time .Results With the increase or decrease of platelet counts , RPA varied correspondingly . With the increase of the concentration of adenosine diphosphate(ADP), collagen (COL) and arachidonic acid(AA), the maxim RPA and the detection rate of aspirin resistance (AR) or clopidogre resistance(CR) increased . For patients with DM , taking different antiplatelet drugs (aspirin or clopidogrel), inducers of ADP, COL and AA were with different influence on RPA . None-fasting and

  20. Effects of Plant Lectins on Human Erythrocyte Agglutination

    Directory of Open Access Journals (Sweden)

    Zubcevic Nadja

    2016-09-01

    Full Text Available Plant lectins are carbohydrate binding proteins or phytohaemagglutinins present in most plants, especially seeds and tubers, which include cereals, potatoes and beans. Lectins have great significance in the diet because of their involvement in gastrointestinal difficulties and erythrocyte agglutination. Blood agglutination activity against A, B, AB and O groups was shown after exposing blood to extracts obtained from 55% of tested plants, while in 45% of plants, agglutination was absent. The results of our study have shown that in humans, 40% of plant extracts exhibited activity against A, 40% of plant extracts exhibited activity against B, and 50% of plant extracts exhibited activity against AB and O groups in humans. The concentration of plant lectins depends on the part of the plant. Lectins from the seeds of certain plants cause the greatest percentage of erythrocyte agglutination, while the lowest agglutination was caused by plant bulbs and leaves. However, lectins derived from all plant species of the family Fabaceae agglutinated erythrocytes of all blood types to some extent.

  1. Characterization of Rubbers from Spherical Punch - Plate Indentation Tests

    Directory of Open Access Journals (Sweden)

    Florina Carmen Ciornei

    2016-12-01

    Full Text Available Rubber plates with different compositions and hardness were tested by continuous indentation, using a spherical punch and hysteretic phenomenon was evidenced. The experimental data interpolation with polynomial functions is accurate and permits estimation of the lost work during loading cycles. The interpolation by power law functions is more convenient by using less parameters and having a form accepted in literature. From the rubbers tested, two were considered to present good damping properties.

  2. Field test for progesterone : an interim evaluation of the sol particle agglutination inhibition assay = Veldtest voor progesteron : een tussentijdse evaluatie van de sol partikel agglutinatie inhibitie test

    NARCIS (Netherlands)

    Davina, J.H.M.

    1987-01-01

    In dit rapport wordt een agglutinatie-test geevalueerd als een variant van de predictor-test voor het controleren van de vruchtbaarheid van grote landbouwhuisdieren. De bepalingsmethode berust op een koppeling van antilichamen tegen progesteron met minuscule gouddeeltjes die in gevriesdroogde toesta

  3. Agglutination of Mouse Erythrocytes by Eperythrozoon coccoides

    OpenAIRE

    Iralu, Vichazelhu; Ganong, Kevin D.

    1983-01-01

    Erythrocytes from blood of mice infected with Eperythrozoon coccoides for 3 or 4 days agglutinated spontaneously. Washed E. coccoides particles agglutinated washed erythrocytes of uninfected mice. E. coccoides-mediated agglutination of normal mouse erythrocytes would be an excellent system for studies of bacterial adhesion.

  4. Agglutination of Mouse Erythrocytes by Eperythrozoon coccoides

    Science.gov (United States)

    Iralu, Vichazelhu; Ganong, Kevin D.

    1983-01-01

    Erythrocytes from blood of mice infected with Eperythrozoon coccoides for 3 or 4 days agglutinated spontaneously. Washed E. coccoides particles agglutinated washed erythrocytes of uninfected mice. E. coccoides-mediated agglutination of normal mouse erythrocytes would be an excellent system for studies of bacterial adhesion. Images PMID:6832825

  5. Agglutination of Mouse Erythrocytes by Eperythrozoon coccoides

    OpenAIRE

    Iralu, Vichazelhu; Ganong, Kevin D.

    1983-01-01

    Erythrocytes from blood of mice infected with Eperythrozoon coccoides for 3 or 4 days agglutinated spontaneously. Washed E. coccoides particles agglutinated washed erythrocytes of uninfected mice. E. coccoides-mediated agglutination of normal mouse erythrocytes would be an excellent system for studies of bacterial adhesion.

  6. Capsular Serotyping of Streptococcus pneumoniae by latex agglutination.

    Science.gov (United States)

    Porter, Barbara D; Ortika, Belinda D; Satzke, Catherine

    2014-09-25

    Latex agglutination reagents are widely used in microbial diagnosis, identification and serotyping. Streptococcus pneumoniae (the pneumococcus) is a major cause of morbidity and mortality world-wide. Current vaccines target the pneumococcal capsule, and there are over 90 capsular serotypes. Serotyping pneumococcal isolates is therefore important for assessing the impact of vaccination programs and for epidemiological purposes. The World Health Organization has recommended latex agglutination as an alternative method to the 'gold standard' Quellung test for serotyping pneumococci. Latex agglutination is a relatively simple, quick and inexpensive method; and is therefore suitable for resource-poor settings as well as laboratories with high-volume workloads. Latex agglutination reagents can be prepared in-house utilizing commercially-sourced antibodies that are passively attached to latex particles. This manuscript describes a method of production and quality control of latex agglutination reagents, and details a sequential testing approach which is time- and cost-effective. This method of production and quality control may also be suitable for other testing purposes.

  7. Color-discrimination threshold determination using pseudoisochromatic test plates

    Directory of Open Access Journals (Sweden)

    Kaiva eJurasevska

    2014-11-01

    Full Text Available We produced a set of pseudoisochromatic plates for determining individual color-difference thresholds to assess test performance and test properties, and analyzed the results. We report a high test validity and classification ability for the deficiency type and severity level (comparable to that of the fourth edition of the Hardy–Rand–Rittler (HRR test. We discuss changes of the acceptable chromatic shifts from the protan and deutan confusion lines along the CIE xy diagram, and the high correlation of individual color-difference thresholds and the red–green discrimination index. Color vision was tested using an Oculus HMC anomaloscope, a Farnsworth D15, and an HRR test on 273 schoolchildren, and 57 other subjects with previously diagnosed red–green color-vision deficiency.

  8. Diagnosis of myocardial infarction based on lectin-induced erythrocyte agglutination: a feasibility study

    Science.gov (United States)

    Bocsi, József; Nieschke, Kathleen; Mittag, Anja; Reichert, Thomas; Laffers, Wiebke; Marecka, Monika; Pierzchalski, Arkadiusz; Piltz, Joachim; Esche, Hans-Jürgen; Wolf, Günther; Dähnert, Ingo; Baumgartner, Adolf; Tarnok, Attila

    2014-03-01

    Myocardial infarction (MI) is an acute life-threatening disease with a high incidence worldwide. Aim of this study was to test lectin-carbohydrate binding-induced red blood cell (RBC) agglutination as an innovative tool for fast, precise and cost effective diagnosis of MI. Five lectins (Ricinus communis agglutinin (RCA), Phaseolus vulgaris erythroagglutinin (PHA), Datura stramonium agglutinin (DSA), Artocarpus agglutinin (ArA), Triticum agglutinin (TA)) were tested for ability to differentiate between agglutination characteristics in patients with MI (n = 101) or angina pectoris without MI (AP) (n = 34) and healthy volunteers (HV) as control (n =68) . RBC agglutination was analyzed by light absorbance of a stirred RBC suspension in the green to red light spectrum in an agglutimeter (amtec, Leipzig, Germany) for 15 min after lectin addition. Mean cell count in aggregates was estimated from light absorbance by a mathematical model. Each lectin induced RBC agglutination. RCA led to the strongest RBC agglutination (~500 RBCs/aggregate), while the others induced substantially slower agglutination and lead to smaller aggregate sizes (5-150 RBCs/aggregate). For all analyzed lectins the lectin-induced RBC agglutination of MI or AP patients was generally higher than for HV. However, only PHA induced agglutination that clearly distinguished MI from HV. Variance analysis showed that aggregate size after 15 min. agglutination induced by PHA was significantly higher in the MI group (143 RBCs/ aggregate) than in the HV (29 RBC-s/aggregate, p = 0.000). We hypothesize that pathological changes during MI induce modification of the carbohydrate composition on the RBC membrane and thus modify RBC agglutination. Occurrence of carbohydrate-lectin binding sites on RBC membranes provides evidence about MI. Due to significant difference in the rate of agglutination between MI > HV the differentiation between these groups is possible based on PHA-induced RBC-agglutination. This novel assay

  9. Detection of Legionella antigenuria by reverse passive agglutination.

    Science.gov (United States)

    Tang, P W; de Savigny, D; Toma, S

    1982-06-01

    A reverse passive agglutination method was developed to detect soluble antigens of Legionella spp. By this method Legionella antigens were detected in urine specimens from 14 of 15 antigenuric patients with clinically diagnosed Legionnaires disease and in none of 263 urine samples from healthy subjects or patients with urinary tract infections. Intra-genus cross-reactivity was observed only between L. pneumophila serogroups 2, 3, and 6. The Legionella reverse passive agglutination method was also evaluated with reference to reagent concentrations, test conditions, and subjectivity of reading test results. The method is rapid and does not require special equipment.

  10. Qualitative and semi-quantitative comparison of an rK39 strip test and direct agglutination test for detection of anti-Leishmania donovani antibodies in the Sudan.

    Science.gov (United States)

    Mansour, Durria; Abass, Elfadil M; Mahamoud, Abdelhafeiz; el Harith, Abdallah

    2009-12-01

    Until now, the comparison of the rK39 strip test (RKT) and direct agglutination test (DAT) for detection of visceral leishmaniasis (VL) is exclusively based on either positive or negative qualification of the reaction outcome. In this study, we compared the diagnostic performance of RKT and DAT for VL both qualitatively and semi-quantitatively. For comparison based on semi-quantitative grounds, the execution of RKT and DAT was according to the standard procedures. For comparison on semi-qualitative grounds with DAT, the RKT was applied to aliquots from positive samples that were two-fold serially diluted in saline to determine, as for the DAT, the end-point reaction in RKT. While qualitatively both RKT and DAT demonstrated comparable reliability for VL detection (sensitivity = 96% and specificity = 98.7% or 99.3%), no significant correlation (r = 0.13) could be established between intensities of their positive reactions in 25 cases studied. A negative correlation was further determined in those 25 VL cases between the positive intensities of the RKT and antibody levels measured semi-quantitatively with the same procedure (r = -0.36) or the DAT (r = -0.30). Irrespective of the low, moderate or high antibody levels measured with RKT ( or = 1:256) or DAT ( or = 1:3,276,800) in patients with confirmed or unconfirmed VL infection, exclusively strong positive intensities were obtained with RKT. For further optimizing diagnosis and simultaneously assessing magnitude of immune response to L. donovani infection in Sudanese patients, the combined application of RKT and DAT is recommended.

  11. Manufacturing High-Fidelity Lunar Agglutinate Simulants

    Science.gov (United States)

    Gutafson, R. J.; Edmunson, J. E.; Rickman, D. L.

    2010-01-01

    The lunar regolith is very different from many naturally occurring material on Earth because it forms in the unique, impact-dominated environment of the lunar surface. Lunar regolith is composed of five basic particle types: mineral fragments, pristine crystalline rock fragments, breccia fragments, glasses of various kinds, and agglutinates (glass-bonded aggregates). Agglutinates are abundant in the lunar regolith, especially in mature regoliths where they can be the dominant component.This presentation will discuss the technical feasibility of manufacturing-simulated agglutinate particles that match many of the unique properties of lunar agglutinates.

  12. Endurance test for mini-plate fuel rig

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Heung June; Yang, Sun Kyu; Park, Jong Man; Kim, Chang Kyu; Ryu, Jeong Soo

    1999-07-01

    This report presents the pressure drop, vibration and endurance test results for mini-plate fuel rig which were designed by KAERI and fabricated by Daewoo Precision Co. From the pressure drop test results, it is noted that the flow velocity across the fuel rig corresponding to the pressure drop of 200 kPa is measured to be about 6.78 kg/sec. Vibration frequency for the fuel rig ranges from 14 to 19 Hz. RMZ (Root Mean Square) displacement for the fuel rig is less than 7 {mu}m, and the maximum displacement is less than 20 {mu}m. Based on the endurance rest results, the appreciable fretting wear for the fuel rig is not observed but the small amount of wear was observed at the interface region between the mini-plates and upper housing cap as well as at the slot regions of a bottom endplate interfacing with the bottom guide arms. (author). 4 refs., 10 tabs., 33 figs.

  13. High Fidelity, High Volume Agglutinate Manufacturing Process Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Up to 65% of the lunar soils are comprised of agglutinates. Although the importance of agglutinate in simulants is often debated, the fact is that agglutinates...

  14. An adjustable parallel-plate capacitor instrument—Test of the theoretical capacitance formula

    Science.gov (United States)

    Wells, Beau; Baker, Emily; Farwell, Austin; Foster, Harrison; Gao, Xiaohan; Gruber, Benjamin; Jones, Erica; Vu, Dennis; Xu, Sonya; Ye, Jingbo

    2016-09-01

    We describe an adjustable parallel-plate capacitor apparatus designed for use in an undergraduate laboratory that permits precise variation of plate separation distances and overlap area. Two experiments are performed with the device to test the ideal capacitor formula derived from Gauss's Law. After correcting for edge effects and minor plate tilt, the device yields capacitance values within 3% of theoretical values.

  15. Comparison of conventional standard tube agglutination test and rose bengal precipitation test in determining brucellosis%常规试管凝集试验与虎红平板试验判断布氏杆菌病的比较

    Institute of Scientific and Technical Information of China (English)

    李玲; 王勤; 曹培义

    2013-01-01

    Objective:To explore a fast,accurate,sensitive test to diagnose brucellosis.Methods:The rose bengal precipitation test (RBPT) and standard tube agglutination test (SAT) were employed.Results:The positive rate of standard tube agglutination test was 35.7%,while that of rose bengal precipitation test was 38.1%,the positive coincidence rate was 93.8%.The rose bengal precipitation test had stronger sensitivity,while the standard tube agglutination test had a higher specificity.Conclusion:Rose bengal precipitation test is suitable for screening and epidemiological research in wide range of brucellosis.%目的:寻找一种快速、准确、敏感性强的试验方法诊断布氏杆菌病.方法:虎红平板凝集试验和试管凝集试验.结果:试管凝集试验阳性检出率为35.7%,虎红平板凝集试验阳性检出率为38.1%,阳性符合率为93.8%.虎红平板凝集试验的灵敏性较强.试管凝集试验有良好的特异性.结论:虎红平板凝集试验适用于对布氏杆菌病大范围的筛查及流行病学调查.

  16. Evaluation of a safranin-O-stained antigen microagglutination test for francisella tularensis antibodies.

    OpenAIRE

    1980-01-01

    A microagglutination test for Francisella tularensis, with 0.025-ml amounts of diluted sera and 0.025-ml amounts of safranin-O-stained antigen in U-bottom microtitration plates, was compared with a tube agglutination test by using 137 sera. There was 86.3% agreement (+/- 1 dilution variation) between the microagglutination results and the tube agglutination results for sera with tube agglutination titers of greater than or equal to 20. There was 100% agreement (+/- 1 dilution variation) for s...

  17. PLATE

    DEFF Research Database (Denmark)

    Kling, Joyce; Hjulmand, Lise-Lotte

    2008-01-01

    ’s level of English is sufficient for the increasing number of courses offered in English each semester. This paper addresses these concerns and describes a pilot project initiated in 2003 at CBS to gauge the overall English language proficiency of those teaching content courses in English. Through......Copenhagen Business School (CBS) finds itself needing to address the issue of English-medium instruction for its increasing number of foreign exchange and full degree students. With internationalisation as a main pillar of the institution’s agenda, there are concerns whether the teaching faculty...... the Project in Language Assessment for Teaching in English (PLATE) language professionals from CBS’s Language Center observe teachers and provide feedback using evaluation criteria from the Common European Framework for Reference (CEFR) supplemented by some additional criteria which take the LSP nature...

  18. PLATE

    DEFF Research Database (Denmark)

    Kling, Joyce; Hjulmand, Lise-Lotte

    2008-01-01

    Copenhagen Business School (CBS) finds itself needing to address the issue of English-medium instruction for its increasing number of foreign exchange and full degree students. With internationalisation as a main pillar of the institution’s agenda, there are concerns whether the teaching faculty......’s level of English is sufficient for the increasing number of courses offered in English each semester. This paper addresses these concerns and describes a pilot project initiated in 2003 at CBS to gauge the overall English language proficiency of those teaching content courses in English. Through...... the Project in Language Assessment for Teaching in English (PLATE) language professionals from CBS’s Language Center observe teachers and provide feedback using evaluation criteria from the Common European Framework for Reference (CEFR) supplemented by some additional criteria which take the LSP nature...

  19. Conventional rapid latex agglutination in estimation of von Willebrand factor: method revisited and potential clinical applications.

    Science.gov (United States)

    Mahat, Marianor; Abdullah, Wan Zaidah; Hussin, Che Maraina Che

    2014-01-01

    Measurement of von Willebrand factor antigen (VWF : Ag) levels is usually performed in a specialised laboratory which limits its application in routine clinical practice. So far, no commercial rapid test kit is available for VWF : Ag estimation. This paper discusses the technical aspect of latex agglutination method which was established to suit the purpose of estimating von Willebrand factor (VWF) levels in the plasma sample. The latex agglutination test can be performed qualitatively and semiquantitatively. Reproducibility, stability, linearity, limit of detection, interference, and method comparison studies were conducted to evaluate the performance of this test. Semiquantitative latex agglutination test was strongly correlated with the reference immunoturbidimetric assay (Spearman's rho = 0.946, P agglutination test and the reference assay. Using the scoring system for the rapid latex test, no agglutination is with 0% VWF : Ag (control negative), 1+ reaction is equivalent to 150% VWF : Ag (when comparing with immunoturbidimetric assay). The findings from evaluation studies suggest that latex agglutination method is suitable to be used as a rapid test kit for the estimation of VWF : Ag levels in various clinical conditions associated with high levels and low levels of VWF : Ag.

  20. Is plate tectonis withstanding the test of time?

    Directory of Open Access Journals (Sweden)

    O. Shields

    1997-06-01

    Full Text Available Since the theory of plate tectonics was first proposed thirty years ago, some problems have arisen in its practical application. These call into question its fundamental assumptions of horizontal plate motion, hotspot fixity, true polar wander, Panthalassa, and the Earth’s constant size while leaving seafloor spreading and subduction intact. A rapidity expanding earth solves these problems and privides an alternative viewpoint worth reconsidering.

  1. A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics

    OpenAIRE

    T.R. Lavich; Cordeiro, R S B; Silva,P.M.R.; M.A. Martins

    2005-01-01

    It is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51ºC and the withdrawal latency for each paw ...

  2. Ridge-spotting: A new test for Pacific absolute plate motion models

    Science.gov (United States)

    Wessel, Paul; Müller, R. Dietmar

    2016-06-01

    Relative plate motions provide high-resolution descriptions of motions of plates relative to other plates. Yet geodynamically, motions of plates relative to the mantle are required since such motions can be attributed to forces (e.g., slab pull and ridge push) acting upon the plates. Various reference frames have been proposed, such as the hot spot reference frame, to link plate motions to a mantle framework. Unfortunately, both accuracy and precision of absolute plate motion models lag behind those of relative plate motion models. Consequently, it is paramount to use relative plate motions in improving our understanding of absolute plate motions. A new technique called "ridge-spotting" combines absolute and relative plate motions and examines the viability of proposed absolute plate motion models. We test the method on six published Pacific absolute plate motions models, including fixed and moving hot spot models as well as a geodynamically derived model. Ridge-spotting reconstructs the Pacific-Farallon and Pacific-Antarctica ridge systems over the last 80 Myr. All six absolute plate motion models predict large amounts of northward migration and monotonic clockwise rotation for the Pacific-Farallon ridge. A geodynamic implication of our ridge migration predictions is that the suggestion that the Pacific-Farallon ridge may have been pinned by a large mantle upwelling is not supported. Unexpected or erratic ridge behaviors may be tied to limitations in the models themselves or (for Indo-Atlantic models) discrepancies in the plate circuits used to project models into the Pacific realm. Ridge-spotting is promising and will be extended to include more plates and other ocean basins.

  3. Test Method for High β Particle Emission Rate of 63Ni Source Plate

    Directory of Open Access Journals (Sweden)

    ZHANG Li-feng

    2015-01-01

    Full Text Available For the problem of measurement difficulties of β particle emission rate of Ni-63 source plate used for Ni-63 betavoltaic battery, a relative test method of scintillation current method was erected according to the measurement principle of scintillation detector.β particle emission rate of homemade Ni-63 source plate was tested by the method, and the test results were analysed and evaluated, it was initially thought that scintillation current method was a feasible way of testing β particle emission rate of Ni-63 source plate with high β particle emission rate.

  4. A study of the incubation of microbead agglutination assays in a microfluidic system

    KAUST Repository

    Castro, David

    2016-12-19

    This work reports on a quantitative study of the incubation of a microbead-based agglutination assay inside a microfluidic system. In this system, a droplet (1.25µL) consisting of a mixture of functionalized microbeads and analyte is flowed through a 0.51mm internal diameter silicone tube. Hydrodynamic forces alone produce a very efficient mixing of the beads within the droplet. We tested the agglutination at different speeds and show a robust response at the higher range of speeds (150 – 200µL/min), while also reaching a completion in the agglutination process. At these velocities, a length of 180cm is shown to be sufficient to confidently measure the agglutination assay, which takes between 2.5 – 3 minutes. This high throughput quantification method has the potential of accelerating the measurements of various types of biomarkers, which can greatly benefit the fields of biology and medicine.

  5. Rapid Detection of Duck Tembusu Virus Antibody by Latex Agglutination Test%乳胶凝集试验方法在检测鸭坦布苏病毒抗体中的应用

    Institute of Scientific and Technical Information of China (English)

    万春和; 傅秋玲; 陈珍; 傅光华; 施少华; 程龙飞; 陈红梅; 黄瑜

    2013-01-01

    以经超速和密度梯度离心浓缩纯化的鸭坦布苏病毒抗原进行方阵滴定,筛选出致敏乳胶的最佳条件,并制备成鸭坦布苏病毒乳胶凝集试验(LAT)用抗原,与特异性的鸭坦布苏病毒阳性血清反应出现肉眼可见的凝集颗粒,进而建立检测鸭坦布苏病毒抗体的乳胶凝集试验方法。经试验测定该致敏的抗原与鸭流感病毒阳性血清、鸭副粘病毒阳性血清、鸭肝炎病毒阳性血清、鸭产蛋综合症病毒阳性血清和鸭呼肠孤病毒阳性血清均不出现凝集现象,表明具有良好的特异性。以建立的LAT 方法和间接 ELISA同时分别对80份鸭血清进行检测,结果LAT方法检出阳性58份、阴性22份,而间接 ELISA 方法检出阳性68份、阴性12份,两者阳性符合率达85.3%。以上结果表明,本方法具有简便、快速、特异等优点,可用于临床样品的快速诊断和血清流行病学调查。%Duck tembusu viruses were cultivated and concentrated by ultracentrifugation and density-gradient centrifugation to determine the best suitable conditions for sensitized latex and prepare antigen for the latex agglutination test (LAT) by titration .The agglutination particles could be observed clearly by naked eyes after antigen reaction with positive duck tembusu virus serum .The sensitized antigen had no cross-reaction with avian influenza virus , avian paramyxovirus type 1 , duck hepatitis virus type 1 , egg drop syndrome virus , and duck reovirus .Of 80 duck serums , 58 and 68 were detected to be positive by the established LAT and ELISA , respectively ;the positive rates for both were above 85.3% with no statistical difference .The results revealed that the established LAT was simple ,quick and specific for the rapid diagnose and survey of duck tembusu virus antibody .

  6. Methods for testing Zernike phase plates and a report on silicon-based phase plates with reduced charging and improved ageing characteristics.

    Science.gov (United States)

    Marko, Michael; Meng, Xing; Hsieh, Chyongere; Roussie, James; Striemer, Christopher

    2013-11-01

    Imaging with Zernike phase plates is increasingly being used in cryo-TEM tomography and cryo-EM single-particle applications. However, rapid ageing of the phase plates, together with the cost and effort in producing them, present serious obstacles to widespread adoption. We are experimenting with phase plates based on silicon chips that have thin windows; such phase plates could be mass-produced and made available at moderate cost. The windows are coated with conductive layers to reduce charging, and this considerably extends the useful life of the phase plates compared to traditional pure-carbon phase plates. However, a compromise must be reached between robustness and transmission through the phase-plate film. Details are given on testing phase-plate performance by means of imaging an amorphous thin film and evaluating the power spectra of the images.

  7. 脑脊液乳胶凝集试验在儿童新型隐球菌性脑膜炎诊断和治疗中的应用%Value of latex agglutination test in the diagnosis and treatment of cryptococcal meningitis in children

    Institute of Scientific and Technical Information of China (English)

    常杏芝; 李若瑜; 王欲琦; 王爽; 熊晖; 吴晔; 包新华; 张月华; 秦炯

    2011-01-01

    目的 探讨脑脊液乳胶凝集试验在隐球菌性脑膜炎诊断和治疗中的应用价值.方法 回顾性分析10例新型隐球菌性脑膜炎患儿的临床资料.通过临床表现,结合脑脊液墨汁染色、乳胶凝集试验与真菌培养,确诊新型隐球菌脑膜炎.随访2~4年抗真菌治疗的效果和乳胶凝集试验滴度变化.结果 10例患儿中,首次脑脊液检查乳胶凝聚试验和/或墨汁染色阳性者8例(乳胶凝集试验滴度1:64~1:1024);另2例中1例在第4次检查时始出现乳胶凝集试验阳性(滴度1:256),1例在第11次检查时墨汁染色阳性而确诊.经正规抗真菌治疗后,6例痊愈,2例死亡,2例失访.治疗后6个月、1年、2年和4年脑脊液乳胶凝集试验仍阳性(滴度1:2~1:16)者分别有6例、3例、2例和1例.结论 脑脊液乳胶凝集试验对于新型隐球菌性脑膜炎的快速诊断具有重要价值.但不宜作为停止抗真菌治疗的依据.%Objective To evaluate the value of cryptococcal latex agglutination test in the diagnosis and treatment of cryptococcal meningitis in children. Methods The clinical data of 10 children with cryptococcal meningitis were retrospectively studied. Cryptococcal meningitis was confirmed based on clinical manifestations, India ink stain, cryptococcal latex agglutination test or cryptococcal culture. The outcome of antifungal treatment and the changes of latex agglutination test titer were followed up for 2 to 4 years. Results Latex agglutination test and/or India ink stain were positive (titer 1:64-1: 1024) in 8 patients in the first examination of cerebrospinal fluid. In the other 2 patients, latex agglutination test was positive ( titer i: 256) in the fourth examination of cerebrospinal fluid in one, and India ink stain was positive in the eleventh examination in the other. After antifungal treatment, six patients were cured, two patients died, and two patients were lost to follow-up. The positive cryptococcal latex

  8. Cosmic ray tests of large area Multigap Resistive Plate Chambers

    CERN Document Server

    An, S; Kim, J; Williams, M C S; Zichichi, A; Zuyeuski, R

    2007-01-01

    We have built Multigap Resistive Plate Chambers (MRPC) with six gas gaps and an active area of . The signals are generated on 2.5 cm wide copper pickup strips; these are read out at each end thus allowing the position of the hit along the strip to be obtained from the time difference. Using three of these chambers we have set up a cosmic tracking system in a similar manner as planned for the Extreme Energy Events (EEE) project. The details of the set-up are presented in this paper. In addition we discuss the time and position resolution of these MRPCs measured using cosmic rays.

  9. Brownian motion of a charged test particle in vacuum between two conducting plates

    Science.gov (United States)

    Yu, Hongwei; Chen, Jun

    2004-12-01

    The Brownian motion of a charged test particle caused by quantum electromagnetic vacuum fluctuations between two perfectly conducting plates is examined and the mean squared fluctuations in the velocity and position of the test particle are calculated. Our results show that the Brownian motion in the direction normal to the plates is reinforced in comparison to that in the single plate case. The effective temperature associated with this normal Brownian motion could be three times as large as that in the single plate case. However, the negative dispersions for the velocity and position in the longitudinal directions, which could be interpreted as reducing the quantum uncertainties of the particle, acquire positive corrections due to the presence of the second plate, and are thus weakened.

  10. Brownian motion of a charged test particle in vacuum between two conducting plates

    CERN Document Server

    Yu, H; Yu, Hongwei; Chen, Jun

    2004-01-01

    The Brownian motion of a charged test particle caused by quantum electromagnetic vacuum fluctuations between two perfectly conducting plates is examined and the mean squared fluctuations in the velocity and position of the test particle are calculated. Our results show that the Brownian motion in the direction normal to the plates is reinforced in comparison to that in the single-plate case. The effective temperature associated with this normal Brownian motion could be three times as large as that in the single-plate case. However, the negative dispersions for the velocity and position in the longitudinal directions, which could be interpreted as reducing the quantum uncertainties of the particle, acquire positive corrections due to the presence of the second plate, and are thus weakened.

  11. Process to create simulated lunar agglutinate particles

    Science.gov (United States)

    Gustafson, Robert J. (Inventor); Gustafson, Marty A. (Inventor); White, Brant C. (Inventor)

    2011-01-01

    A method of creating simulated agglutinate particles by applying a heat source sufficient to partially melt a raw material is provided. The raw material is preferably any lunar soil simulant, crushed mineral, mixture of crushed minerals, or similar material, and the heat source creates localized heating of the raw material.

  12. 78 FR 33132 - Quality Verification for Plate-Type Uranium-Aluminum Fuel Elements for Use in Research and Test...

    Science.gov (United States)

    2013-06-03

    ... COMMISSION Quality Verification for Plate-Type Uranium-Aluminum Fuel Elements for Use in Research and Test... Verification for Plate-Type Uranium-Aluminum Fuel Elements for Use in Research and Test Reactors.'' This guide... plate-type uranium-aluminum fuel elements used in research and test reactors (RTRs). ADDRESSES: Please...

  13. Guarded Flat Plate Cryogenic Test Apparatus and Calorimeter

    Science.gov (United States)

    Fesmire, James E. (Inventor); Johnson, Wesley L. (Inventor)

    2017-01-01

    A test apparatus for thermal energy measurement of disk-shaped test specimens has a cold mass assembly locatable within a sealable chamber with a guard vessel having a guard chamber to receive a liquid fluid and a bottom surface to contact a cold side of a test specimen, and a test vessel having a test chamber to receive a liquid fluid and encompassed on one side by a center portion of the bottom surface shared with the guard vessel. A lateral wall assembly of the test vessel is closed by a vessel top, the lateral wall assembly comprising an outer wall and an inner wall having opposing surfaces that define a thermal break including a condensable vapor pocket to inhibit heat transfer through the lateral wall from the guard vessel to the test vessel. A warm boundary temperature surface is in thermal communication with a lower surface of the test specimen.

  14. Results of fracture mechanics tests on PNC SUS 304 plate

    Energy Technology Data Exchange (ETDEWEB)

    Mills, W.J.; James, L.A.; Blackburn, L.D.

    1985-08-01

    PNC provided SUS 304 plate to be irradiated in FFTF at about 400/sup 0/C to a target fluence of 5 x 10/sup 21/ n/cm/sup 2/ (E > 0.1 MeV). The actual irradiation included two basically different exposure levels to assure that information would be available for the exposure of interest. After irradiation, tensile properties, fatigue-crack growth rates and J-integral fracture toughness response were determined. These same properties were also measured for the unirradiated material so radiation damage effects could be characterized. This report presents the results of this program. It is expected that these results would be applicable for detailed fracture analysis of reactor components. Recent advances in elastic-plastic fracture mechanics enable reasonably accurate predictions of failure conditions for flawed stainless steel components. Extensive research has focused on the development of J-integral-based engineering approach for assessing the load carrying capacity of low-strength, high-toughness structural materials. Furthermore, Kanninen, et al., have demonstrated that J-integral concepts can accurately predict the fracture response for full-scale cracked structures manufactured from Type 304 stainless steel.

  15. Development of nondestructive testing techniques for plated-through holes in multilayer printed circuit boards

    Science.gov (United States)

    Anthony, P. L.; Mcmurtrey, J. E.

    1971-01-01

    The development of a nondestructive test with the capability to interrogate plated-through holes as small as 0.51 millimeters inside diameter is discussed. The system can detect defects such as holes, voids, cracks, and thin spots that reduce the current carrying capability of plates-through interconnects by 20 percent or more. Efforts were directed toward the design and fabrication of magnetic circuitry mutual coupling probes and to evaluate the effectiveness of these devices for detecting in multilayer board plated-through holes.

  16. Rapid serum agglutination and agar gel immunodiffusion tests associated to clinical signs in rams experimentally infected with Brucella ovis Teste de soro aglutinação rápida e do teste de imunodifusão em gel de ágar associados aos sinais clínicos em carneiros infectados experimentalmente com Brucella ovis

    Directory of Open Access Journals (Sweden)

    Cristiane Nakada Nozaki

    2011-08-01

    Full Text Available The purpose of this study was to evaluate the agar gel immunodiffusion and the rapid serum agglutination tests associated to clinical signs in rams experimentally infected with Brucella ovis. The serological profile during the 12 months of infection showed a large fluctuation of antibodies that favors the failure in the diagnostic. The evaluation of tests after the experimental infection allowed to suggest that none of the tests were able to detect the infection throughout the period of study. The study reinforces the importance of considering the clinical signs to support the diagnosis of Brucella ovis infection in rams.O objetivo deste estudo foi avaliar o uso do teste de imunodifusão em gel de ágar e o teste sorológico de aglutinação rápida comparados aos sinais clínicos em carneiros infectados experimentalmente com Brucella ovis para o diagnóstico confirmatório da brucelose ovina. O perfil sorológico durante os 12 meses pós-infecção mostrou flutuação da resposta por anticorpos, que favorece a falha no diagnóstico. A avaliação dos testes indicou que nenhum dos testes foi capaz de detectar a infecção durante todo o período de estudo. O estudo ressalta a importância de considerar os sinais clínicos para apoiar o diagnóstico confirmatório da infecção por Brucella ovis em carneiros.

  17. Assembly and tests of Resistive Plate Chambers (RPC) for the CMS barrel detector

    CERN Multimedia

    Gabriella Pugliese, Bari

    2006-01-01

    The pictures 1-3 show some assembly steps of Resistive Plate Chambers in Bari and their tests with cosmic rays (4-6: moving RPC to the test towers; 7-8 cosmic tests). Pictures 9-10 show assembly and tests in Sofia, 11 shows RPC coupling with drift tubes at ISR and 12-13 tests at the CMS site at CERN.

  18. Extracellular calcium is involved in egg yolk-induced head-to-head agglutination of bull sperm.

    Science.gov (United States)

    Yang, D H; McMillan, A G; Standley, N T; Shannon, P; Xu, Z Z

    2012-10-15

    Head-to-head agglutination of bull sperm occurs when semen is highly diluted in an egg yolk-citrate diluent without streptomycin. The objectives were to investigate causes of sperm agglutination and the underlying mechanism. Aliquots of bull semen were diluted in a base diluent (BD) supplemented with various test components and the percentage of agglutinated sperm (% AggSp) was quantified at 1, 5, 24, 48, and 72 h of incubation. When sperm were incubated at 22 °C, no agglutination was observed in BD for up to 72 h, whereas the % AggSp was 5.0, 41.7, 72.2, 91.1, and 92.8% in BD + 5% egg yolk (BD + EY) at 1, 5, 24, 48 and 72 h, respectively. However, no sperm agglutination was observed in BD + EY if incubation temperature was 37 °C. Addition of 5 or 10 mM ethylenebis (oxyethyleneni-trilo) tetra-acetic acid to BD + EY reduced the % AggSp from 95% to sperm agglutination in the absence of egg yolk, implicating calcium and other factors in egg yolk. Addition of the citrate-soluble fraction (CSF) of egg yolk to BD induced sperm agglutination similar to whole egg yolk, whereas water- and saline-soluble fractions of egg yolk were ineffective. The sperm-agglutinating efficacy of CSF (the % AggSp = 95% at 72 h) was reduced by dialysis (20%; P sperm agglutination, whereas addition of 2 mM of a cAMP analogue, dbcAMP, to a nonagglutinating diluent failed to induce sperm agglutination. Agglutination status had no effect on sperm plasma membrane/acrosome status and mitochondrial membrane potential. In conclusion, calcium and other component(s) in the CSF of egg yolk induced head-to-head agglutination of bull sperm in a time- and temperature-dependent manner. Although the mechanism of agglutination was not determined, the cAMP- protein kinase A signaling pathway was not involved.

  19. 81例微柱凝胶法交叉配血试验次侧凝集的临床因素分析%Analysis of clinical factors for hypo-side agglutination in 81 cases in cross match blood test with microcolumn gel assay

    Institute of Scientific and Technical Information of China (English)

    蒯迪文; 郭黠

    2009-01-01

    Objective To analyze the etiological factor for hypo-side agglutination in cross match blood test(CMT)with microcolumn gel assay,and to provide a guide to the clinical blood transfusion.Methods The data were collected and analyzed about 81 cases with hypo-side agglutination in CMT with microcolumn gel assay and direct antiglobulin test(DAT)positive from Jan.2007 to Oct.2008.Results Among the 81 hype-side agglutinated cases,most were with kidney disease,liver and gall disease,hematologic disease and immunologic disease.Specially,the kidney disease was most,accounting for 16.2%.Conclusion The analysis contributes to disposal in CMT and the safety of clinical blood transfusion.%目的 分析使用微柱凝胶法进行交叉配血试验出现次侧凝集的各种病因,为临床输血提供指导作用.方法 收集本院2007年1月至2008年10月间所有使用微柱凝胶法进行交叉配血试验次侧出现凝集,且患者红细胞直接Coombs试验抗体为阳性的患者并进行统计分析.结果 81例次侧凝集的患者中大部分为肾脏疾病、肝胆疾病、血液疾病以及免疫性疾病,其中又以肾脏疾病最多,占16.2%.结论 本结论对不同疾病的交叉配血试验和安全输血有一定的指导作用.

  20. A reciprocating pin-on-plate test-rig for studying friction materials for holding brakes

    DEFF Research Database (Denmark)

    Poulios, Konstantinos; Drago, Nicola; Klit, Peder

    2014-01-01

    -on-plate test-rig for studying the evolution of wear by monitoring the pin height reduction using Eddy-current proximity sensors is presented. Moreover, a new mechanism for recording the friction force is suggested. Apart from the design of the test-rig, friction force and wear rate measurements for two...

  1. Functions for patch test in finite element analysis of the Mindlin plate and the thin cylindrical shell

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Current patch test for Mindlin plate element only satisfies the zero shear deformation condition.The patch test of non-zero constant shear for Mindlin plate problem cannot be performed.For shell element, the patch test does not even exist.Based on the theory of enhanced patch test proposed by Chen W J (2006),the authors proposed the enhanced patch test function for Mindlin plate and thin cylindrical shell elements.This enhanced patch test function can be used to assess the convergence of the Mindlin plate and cylindrical thin shell elements.

  2. Innovative design of composite structures: Design, manufacturing, and testing of plates utilizing curvilinear fiber trajectories

    Science.gov (United States)

    Hyer, M. W.; Rust, R. J.; Waters, W. A., Jr.

    1994-01-01

    As a means of improving structural design, the concept of fabricating flat plates containing holes by incorporating curvilinear fiber trajectories to transmit loads around the hole is studied. In the present discussion this concept is viewed from a structural level, where access holes, windows, doors, and other openings are of significant size. This is opposed to holes sized for mechanical fasteners. Instead of cutting the important load-bearing fibers at the hole edge, as a conventional straightline design does, the curvilinear design preserves the load-bearing fibers by orienting them in smooth trajectories around the holes, their loading not ending abruptly at the hole edge. Though the concept of curvilinear fiber trajectories has been studied before, attempts to manufacture and test such plates have been limited. This report describes a cooperative effort between Cincinnati Milacron Inc., NASA Langley Research Center, and Virginia Polytechnic Institute and State University to design, manufacture, and test plates using the curvilinear fiber trajectory concept. The paper discusses details of the plate design, details of the manufacturing, and a summary of results from testing the plates with inplane compressive buckling loads and tensile loads. Comparisons between the curvilinear and conventional straightline fiber designs based on measurements and observation are made. Failure modes, failure loads, strains, deflections, and other key responses are compared.

  3. High Velocity Tensile Test for Thin Plate Specimen with One Bar Method

    Science.gov (United States)

    Itabashi, Masaaki

    In order to design thin-walled impact-resistant structure, for example, an automotive body, dynamic behavior of thin plate is essential. So far, except for laminated composite materials, high velocity tensile test of thin plate specimen did not attract impact researchers' and engineers' attention very much. In this paper, the previous thin plate specimen assembly for the one bar method was improved. The one bar method has been utilized for cylindrical specimens of various solid materials and is known as an effective high velocity tensile testing technique. Unfortunately, the previous assembly introduced a tremendous initial peak on stress-strain curves, even for aluminum alloys. With a new specimen assembly, stress-strain curves for IF (Interstitial-atom Free) steel and 7075-T6 aluminum alloy obtained by the one bar method were almost equivalent to those obtained by the tensile version of the split Hopkinson pressure bar method.

  4. Industrial Test of High Strength Steel Plates Free Boron Q890D Used for Engineering Machinery

    Science.gov (United States)

    Dong, Ruifeng; Liu, Zetian; Gao, Jun

    The chemistry composition, process parameters and the test results of Q890D free boron high strength steel plate used for engineering machinery was studied. The 16 40 mm thickness steel plates with good mechanical properties that was yield strength of 930 970 MPa, tensile strength of 978 1017 MPa, elongation of 13.5 15%, the average impact energy value of more than 100 J were developed by improving steel purity, adopting the reasonable controlled rolling and cooling process, using reasonable off-line quenching and tempering process. The test plates have good crack resistance in 60 °C preheat temperature condition because of that there are no any cracks in the surfaces, cross-section and roots of welding joints.

  5. Digitizer of astronomical plates at Shanghai Astronomical Observatory and its performance test

    Science.gov (United States)

    Yu, Yong; Zhao, Jian-Hai; Tang, Zheng-Hong; Shang, Zheng-Jun

    2017-02-01

    Before CCD detectors were widely employed in observational astronomy, the main method of detection was the use of glass astrophotographic plates. Astronomical plates have been used to record information on the position and activity of celestial bodies for more than 100 years. There are about 30 000 astronomical plates in China, and the digitization of astronomical plates is of great significance for permanent preservation and to make full use of these valuable observation data. A digitizer with high precision and high measuring speed is a key piece of equipment for carrying out the task of digitizing these astronomical plates. A digitizer for glass astrophotographic plates was developed jointly by Shanghai Astronomical Observatory and Nishimura Co., Ltd of Japan. The digitizer’s hardware was manufactured by Nishimura Co., Ltd, and the performance test, error corrections as well as image processing of the digitizer were carried out by Shanghai Astronomical Observatory. The main structure and working mode of the digitizer are introduced in this paper. A performance test shows that brightness uniformity of illumination within the measuring area is better than 0.15%, the repeatability of digitized positions is better than 0.2 µm and the repeatability of digitized brightness is better than 0.01 instrumental magnitude. The systematic factors affecting digitized positions, such as lens distortion, the actual optical resolution, non-linearity of guide rails, non-uniformity of linear motors in the mobile platform, deviation of the image mosaic, and non-orthogonality between the direction of scanning and camera linear array, are calibrated and evaluated. Based on an astronomical plate with a size of 300mm × 300mm, which was digitized at different angles, the conversion residuals of positions of common stars on different images were investigated. The results show that the standard deviations of the residuals are better than 0.9 µm and the residual distribution is almost

  6. Mass-based readout for agglutination assays

    Science.gov (United States)

    Chunara, Rumi; Godin, Michel; Knudsen, Scott M.; Manalis, Scott R.

    2007-11-01

    We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.

  7. Beam test results on double-gap resistive plate chambers proposed for CMS experiment

    Science.gov (United States)

    Abbrescia, M.; Bruno, G.; Colaleo, A.; Iaselli, G.; Lamanna, G.; Loddo, F.; Maggi, M.; Marangelli, B.; Natali, S.; Nuzzo, S.; Pugliese, G.; Ranieri, A.; Romano, F.; Gianini, G.; Ratti, S. P.; Vitulo, P.

    Resistive Plate Chambers were tested in a muon and pion beam to study the performances at different running conditions. Results on a first chamber built without the linseed oil treatment of the bakelite surfaces are presented together with an evaluation of the local effects due to the spacers. These results are extrapolated to the conditions expected at LHC.

  8. Beam Test Results on Resistive Plate Chambers for the CMS Experiment

    CERN Document Server

    Abbrescia, Marcello; Calaeo, A; Iaselli, Giuseppe; Lammana, G; Loddo, Flavio; Maggi, Marcello; Marangelli, Bartolomeo; Natali, Sergio; Nuzzo, Salvatore; Puglise, G; Ranieri, Riccardo; Ratti, Sergio P; Romano, Francesco; Vitulo, Paolo

    1998-01-01

    Resistive Plate Chambers were tested in a muon and a pion beam to study the performances at different running conditions. Results on a first chamber built without the linseed oil treatment of the bakelite surfaces are presented together with an evaluation of the local effects dut to spacers. These results are extrapolated to the conditions expected at LHC.

  9. Penicillamine prevents ram sperm agglutination in media that support capacitation.

    Science.gov (United States)

    Leahy, T; Rickard, J P; Aitken, R J; de Graaf, S P

    2016-02-01

    Ram spermatozoa are difficult to capacitate in vitro. Here we describe a further complication, the unreported phenomenon of head-to-head agglutination of ram spermatozoa following dilution in the capacitation medium Tyrodes plus albumin, lactate and pyruvate (TALP). Sperm agglutination is immediate, specific and persistent and is not associated with a loss of motility. Agglutination impedes in vitro sperm handling and analysis. So the objectives of this study were to investigate the cause of sperm agglutination and potential agents which may reduce agglutination. The percentage of non-agglutinated, motile spermatozoa increased when bicarbonate was omitted from complete TALP suggesting that bicarbonate ions stimulate the agglutination process. d-penicillamine (PEN), a nucleophilic thiol, was highly effective at reducing agglutination. The inclusion of 250 μM PEN in TALP reduced the incidence of motile, agglutinated spermatozoa from 76.7 ± 2.7% to 2.8 ± 1.4%. It was then assessed if PEN (1 mM) could be included in existing ram sperm capacitation protocols (TALP +1 mM dibutyryl cAMP, caffeine and theophylline) to produce spermatozoa that were simultaneously capacitated and non-agglutinated. This protocol resulted in a sperm population which displayed high levels of tyrosine phosphorylated proteins and lipid disordered membranes (merocyanine-540) while remaining motile, viable, acrosome-intact and non-agglutinated. In summary, PEN (1 mM) can be included in ram sperm capacitation protocols to reduce sperm agglutination and allow for the in vitro assessment of ram sperm capacitation.

  10. Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications

    Directory of Open Access Journals (Sweden)

    Marianor Mahat

    2014-01-01

    Full Text Available Measurement of von Willebrand factor antigen (VWF : Ag levels is usually performed in a specialised laboratory which limits its application in routine clinical practice. So far, no commercial rapid test kit is available for VWF : Ag estimation. This paper discusses the technical aspect of latex agglutination method which was established to suit the purpose of estimating von Willebrand factor (VWF levels in the plasma sample. The latex agglutination test can be performed qualitatively and semiquantitatively. Reproducibility, stability, linearity, limit of detection, interference, and method comparison studies were conducted to evaluate the performance of this test. Semiquantitative latex agglutination test was strongly correlated with the reference immunoturbidimetric assay (Spearman’s rho = 0.946, P150% VWF : Ag (when comparing with immunoturbidimetric assay. The findings from evaluation studies suggest that latex agglutination method is suitable to be used as a rapid test kit for the estimation of VWF : Ag levels in various clinical conditions associated with high levels and low levels of VWF : Ag.

  11. 77 FR 16868 - Quality Verification for Plate-Type Uranium-Aluminum Fuel Elements for Use in Research and Test...

    Science.gov (United States)

    2012-03-22

    ... COMMISSION Quality Verification for Plate-Type Uranium-Aluminum Fuel Elements for Use in Research and Test...-Type Uranium-Aluminum Fuel Elements for Use in Research and Test Reactors,'' is temporarily identified... verifying the quality of plate-type uranium-aluminum fuel elements used in research and test reactors (RTRs...

  12. Towards a high throughput droplet-based agglutination assay

    KAUST Repository

    Kodzius, Rimantas

    2013-10-22

    This work demonstrates the detection method for a high throughput droplet based agglutination assay system. Using simple hydrodynamic forces to mix and aggregate functionalized microbeads we avoid the need to use magnetic assistance or mixing structures. The concentration of our target molecules was estimated by agglutination strength, obtained through optical image analysis. Agglutination in droplets was performed with flow rates of 150 µl/min and occurred in under a minute, with potential to perform high-throughput measurements. The lowest target concentration detected in droplet microfluidics was 0.17 nM, which is three orders of magnitude more sensitive than a conventional card based agglutination assay.

  13. Performance test of a plate-type ultrasonic waveguide sensor in sodium

    Science.gov (United States)

    Joo, Young-Sang; Bae, Jin-Ho; Kim, Hoe-Woong; Kim, Jong-Bum

    2013-01-01

    A plate-type ultrasonic waveguide sensor that uses the A0-mode leaky Lamb waves has been developed for under-sodium viewing of the reactor core and in-vessel structures in the sodiumcooled fast reactor. A new ultrasonic waveguide sensor that has beryllium (Be) and nickel (Ni) coating layers on its surfaces is proposed for the enhancement of the ultrasonic beam radiation and wetting capabilities in sodium. The sodium test facility with a glove box system and a sodium test loop has been designed and constructed to carry out the performance test of the developed ultrasonic waveguide sensor in a sodium environment. A 1.7 m long ultrasonic waveguide sensor with beryllium and nickel coating layers was designed and manufactured. The performance tests were carried out to evaluate the ultrasonic beam radiation characteristics and C-scan imaging resolution of the plate-type ultrasonic waveguide sensor in sodium.

  14. A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics

    Directory of Open Access Journals (Sweden)

    T.R. Lavich

    2005-03-01

    Full Text Available It is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP test to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51ºC and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 µg/paw and mice (125-500 µg/paw with carrageenan led to a rapid hyperalgesic response of the ipsilateral paw that reached a plateau from 15 to 360 min after challenge. Pretreatment with indomethacin (4 mg/kg, ip inhibited the phenomenon at all the times analyzed. Similarly, plantar stimulation of rats and mice with prostaglandin E2 (0.5 and 1 µg/paw also resulted in rapid hyperalgesia which was first detected 15 min post-challenge. Finally, we observed that the MHP test was more sensitive than the classical Hargreaves' test, being able to detect about 4- and 10-fold lower doses of prostaglandin E2 and carrageenan, respectively. In conclusion, the MHP test is a simple and sensitive method for detecting peripheral hyperalgesia and analgesia in rats and mice. This test represents a low-cost alternative for the study of inflammatory pain in freely moving animals.

  15. A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics.

    Science.gov (United States)

    Lavich, T R; Cordeiro, R S B; Silva, P M R; Martins, M A

    2005-03-01

    It is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51 degrees C and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 microg/paw) and mice (125-500 microg/paw) with carrageenan led to a rapid hyperalgesic response of the ipsilateral paw that reached a plateau from 15 to 360 min after challenge. Pretreatment with indomethacin (4 mg/kg, i.p.) inhibited the phenomenon at all the times analyzed. Similarly, plantar stimulation of rats and mice with prostaglandin E2 (0.5 and 1 microg/paw) also resulted in rapid hyperalgesia which was first detected 15 min post-challenge. Finally, we observed that the MHP test was more sensitive than the classical Hargreaves' test, being able to detect about 4- and 10-fold lower doses of prostaglandin E2 and carrageenan, respectively. In conclusion, the MHP test is a simple and sensitive method for detecting peripheral hyperalgesia and analgesia in rats and mice. This test represents a low-cost alternative for the study of inflammatory pain in freely moving animals.

  16. A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis

    Directory of Open Access Journals (Sweden)

    Mehmet Özdemir, Bahadır Feyzioğlu, Muhammed Güzel Kurtoğlu, Metin Doğan, Hatice Türk Dağı, Şerife Yüksekkaya, Recep Keşli, Bülent Baysal

    2011-01-01

    Full Text Available Background: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and immuncapture agglutination tests with Coombs anti-brucella test.Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were included into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Immuncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated.Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test.Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis.

  17. Plate coil thermal test bench for the Daniel K. Inouye Solar Telescope (DKIST) carousel cooling system

    Science.gov (United States)

    Phelps, LeEllen; Murga, Gaizka; Montijo, Guillermo; Hauth, David

    2014-08-01

    Analyses have shown that even a white-painted enclosure requires active exterior skin-cooling systems to mitigate dome seeing which is driven by thermal nonuniformities that change the refractive index of the air. For the Daniel K. Inouye Solar Telescope (DKIST) Enclosure, this active surface temperature control will take the form of a system of water cooled plate coils integrated into the enclosure cladding system. The main objective of this system is to maintain the surface temperature of the enclosure as close as possible to, but always below, local ambient temperature in order to mitigate this effect. The results of analyses using a multi-layer cladding temperature model were applied to predict the behavior of the plate coil cladding system and ultimately, with safety margins incorporated into the resulting design thermal loads, the detailed designs. Construction drawings and specifications have been produced. Based on these designs and prior to procurement of the system components, a test system was constructed in order to measure actual system behavior. The data collected during seasonal test runs at the DKIST construction site on Haleakalā are used to validate and/or refine the design models and construction documents as appropriate. The test fixture was also used to compare competing hardware, software, components, control strategies, and configurations. This paper outlines the design, construction, test protocols, and results obtained of the plate coil thermal test bench for the DKIST carousel cooling system.

  18. Systems, devices, and methods for agglutination assays using sedimentation

    Science.gov (United States)

    Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.

    2016-01-26

    Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.

  19. Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant.

    Science.gov (United States)

    Bhangale, Amit; Pathak, Amardeep; Pawar, Smita; Jeloka, Tarun

    2017-01-01

    Measurement of alloantibody titer to a red cell antigen (ABO titers) is an integral part of management of ABO incompatible kidney transplants (ABOiKT). There are different methods of titer estimation. Alloantibody detection by tube titration and Gel agglutination columns are accepted methodologies. It is essential to find the difference in titers between the two methods so as to set the 'cut-off' titer accordingly, depending upon the method used. We did a prospective observational study to compare and correlate the ABO titers using these two different techniques - conventional tube technique (CTT) and the newer column agglutination technique (CAT). A total of 67 samples were processed in parallel for anti-A/B antibodies by both tube dilution and column agglutination methods. The mean titer by conventional tube method was 38.5 + 96.6 and by the column agglutination test was 96.4 + 225. The samples correlated well with Spearman rho correlation coefficient of 0.94 (P = 0.01). The column agglutination method for anti A/B titer estimation in an ABO incompatible kidney transplant is more sensitive, with the column agglutination results being approximately two and half fold higher (one more dilution) than that of tube method.

  20. MEMS reagent and sample handling procedure: Feasibility of viral antibody detection by passive immune agglutination

    Science.gov (United States)

    Bailey, G. D.; Tenoso, H. J.

    1975-01-01

    An attempt was made to develop a test requiring no preadsorption steps for the assessment of antibodies to rubella and mumps viruses using the passive immune agglutination (PIA) method. Both rubella and mumps antigens and antibodies were prepared. Direct PIA tests, using rubella antigen-coated beads, and indirect PIA tests, using rubella antibody-coated beads, were investigated. Attempts, using either method, were unsuccessful. Serum interference along with nonspecific agglutination of beads by the rubella antigen resulted in no specific response under the test conditions investigated. A new, highly sensitive approach, the enzyme immunoassay (EIA) test system, is recommended to overcome the nonspecificity. This system is a logical outgrowth of some of the solid phase work done on MEMS and represents the next generation tests system that can be directly applied to early disease detection and monitoring.

  1. Testing the Corrosion Behaviour of Plated Aluminum Strips for Heat Exchangers Operating in the Automotive Industry

    Science.gov (United States)

    Nowak, Marek; Opyrchał, Mieczysław; Kłyszewski, Andrzej; Żelechowski, Janusz

    The automotive industry uses heat exchangers made of aluminum alloys. The radiators for an engine cooling system comprise elements (tubes) in which the coolant flows. These elements are made of AlMn alloys (e.g. AlMn or AlMnCu), while ribs are made from the sheet metal plated with AlSi alloys. While in service, the heat exchangers are exposed to harsh weather conditions, hence the need to ensure that the individual elements of the design are adequately protected from corrosion. One of the ways to obtain this protection is by modification of the chemical composition of aluminum alloys used in the manufacture of radiators. In this study, corrosion tests were carried out to characterise the investigated materials in terms of their corrosive behavior. The experiments included measurements of corrosion potential, testing of corrosion resistance in neutral salt spray, and electrochemical studies of selected plated AlMn alloy strips with different chemical composition.

  2. Standardized solar simulator tests of flat plate solar collectors. 1: Soltex collector with two transparent covers

    Science.gov (United States)

    Simon, F.

    1975-01-01

    A Soltex flat plate solar collector was tested with a solar simulator for inlet temperatures of 77 to 201 F, flux levels of 240 and 350 Btu/hr-sq ft, a collant flow rate of 10.5 lb/hr sq ft, and incident angles of 0 deg, 41.5 deg, and 65.2 deg. Collector performance is correlated in terms of inlet temperature, flux level, and incident angle.

  3. Direct tests of micro channel plates as the active element of a new shower maximum detector

    Energy Technology Data Exchange (ETDEWEB)

    Ronzhin, A., E-mail: ronzhin@fnal.gov [Fermilab, Batavia, IL 60510 (United States); Los, S.; Ramberg, E. [Fermilab, Batavia, IL 60510 (United States); Apresyan, A.; Xie, S.; Spiropulu, M. [California Institute of Technology, Pasadena, CA (United States); Kim, H. [University of Chicago, Chicago, IL 60637 (United States)

    2015-09-21

    We continue the study of micro channel plates (MCP) as the active element of a shower maximum (SM) detector. We present below test beam results obtained with MCPs detecting directly secondary particles of an electromagnetic shower. The MCP efficiency to shower particles is close to 100%. The time resolution obtained for this new type of the SM detector is at the level of 40 ps.

  4. Application of patch test in meshless analysis of continuously non-homogeneous piezoelectric circular plate

    Directory of Open Access Journals (Sweden)

    Staňák P.

    2013-06-01

    Full Text Available Proposed paper presents application of the patch test for meshless analysis of piezoelectric circular plate with functionally graded material properties. Functionally graded materials (FGM are the special class of composite materials with continuous variation of volume fraction of constituents in predominant direction. Patch test analysis is an important tool in numerical methods for addressing the convergence. Meshless local Petrov-Galerkin (MLPG method together with moving least-squares (MLS approximation scheme is applied in the analysis. No finite elements are required for approximation or integration of unknown quantities. Circular plate is considered as a 3-D axisymmetric piezoelectric solid. Considering the axial symmetry, the problem is reduced to a 2-dimensinal one. Displacement and electric potential fields are prescribed on the outer boundaries in order to reach the state of constant stress field inside the considered plate as required by the patch test and the governing equations. Values of prescribed mechanical and electrical fields must be determined in order to comply with applied FGM gradation rule. Convergence study is performed to assess the considered meshless approach and several conclusions are finally presented.

  5. [The latex agglutination with video digital registration: the enhancement of diagnostic significance of conventional technique].

    Science.gov (United States)

    Starovoĭtova, T A; Steriopolo, N A; Zaĭko, V V; Vengerov, Iu Iu

    2012-02-01

    The rapid semiquantitative latex-tests, because of their analytic characteristics and convenient application, became widespread in the practice of laboratory diagnostics. Though, in spite of high sensitivity and specificity, their diagnostic effectiveness is lower that it could be mainly because of the impossibility to document the results of latex agglutinative re4actions and to manage the objective quality control. The application of systems of video digital registration permits to enhance the clinical significance of these analyses. By means of scanner systems (control and program complex "Expert Lab") the image of analytic objects is received with the results of latex agglutination reaction. The application of program techniques (the programs "Expert Lab - Agglutination" and "Expert Lab - Agglutination - Micros") in data processing permits to get the precise qualitative characteristics of active reactions, to ensure the automatic interpretation of results and gives an opportunity to proceed with the internal laboratory quality control. The saving of analytic object image in computer memory after termination of reaction favors the formation of data base, the implementation of retrospective evaluation of obtained results, additional consultations in dubious cases, including on-line. The application of complex "Expert Lab" permitted to develop the miniaturizes matrix systems permitting to decrease the withdrawal of latex reagents, to increase the productivity of analytical stage of operation preserving all analytical characteristics of method.

  6. Comparison of the indirect fluorescent antibody test and modified agglutination test for detection of anti-Toxoplasma gondii antibodies in rats / Comparação da reação de imunofluorescência indireta e do teste de aglutinação modificado na detecção de anticorpos anti-Toxoplasma gondii em ratos

    Directory of Open Access Journals (Sweden)

    Roberta Lemos Freire

    2010-09-01

    Full Text Available The toxoplasmosis is a zoonosis caused by Toxoplasma gondii and affects a lot of species of carnivores and omnivores, including the human. The rodents are important in the transmition cycle because they act as an infection font to felines, the definitive host of this protozoan. The objective of this work was to evaluate the Modified Agglutination Test (MAT for the serologic diagnosis of toxoplasmosis in rats, comparing with the Indirect Fluorescent Antibody Test (IFAT, which has been considered the golden standard in animal toxoplasmosis diagnosis. Kappa test was used for comparing the serologic tests (IFAT and MAT and for determination of cutoff appropriate to MAT in this animal species. 182 rats were caught on local recycling of solid waste and solid residue storage in Londrina city, Paraná. Out of the 182 rats, nine (4.94% were positive to IFAT at a dilution of 1:16, and 17 (9.34% and five (2.75% were reactive to MAT in dilutions 1:25 and 1:50, respectively. The comparison of results between the techniques presented kappa coefficients of 0.26 and 0.55, respectively at 1:25 and 1:50 dilutions of MAT. It can be concluded that the dilution 1:50 is the most suitable to be used as cutoff for detecting T. gondii antibodies in rats using MAT, because agreed with IFAT.A toxoplasmose é uma zoonose causada pelo Toxoplasma gondii que acomete várias espécies carnívoras e onívoras, incluindo o ser humano. Os roedores são importantes na cadeia epidemiológica da doença por servirem de fonte de infecção aos felídeos, os hospedeiros definitivos deste protozoário. O objetivo deste trabalho foi avaliar o Teste de Aglutinação Modificada (MAT na detecção de anticorpos contra T. gondii em ratos, comparando-o à Reação de Imunofluorescência Indireta (RIFI, considerada padrão ouro para o diagnóstico da toxoplasmose animal. Empregou-se o teste kappa para a comparação dos testes sorológicos (RIFI e MAT e para a determinação do ponto de corte

  7. Standard Test Method for Mechanical Hydrogen Embrittlement Evaluation of Plating/Coating Processes and Service Environments

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2010-01-01

    1.1 This test method describes mechanical test methods and defines acceptance criteria for coating and plating processes that can cause hydrogen embrittlement in steels. Subsequent exposure to chemicals encountered in service environments, such as fluids, cleaning treatments or maintenance chemicals that come in contact with the plated/coated or bare surface of the steel, can also be evaluated. 1.2 This test method is not intended to measure the relative susceptibility of different steels. The relative susceptibility of different materials to hydrogen embrittlement may be determined in accordance with Test Method F1459 and Test Method F1624. 1.3 This test method specifies the use of air melted AISI E4340 steel per SAE AMS-S-5000 (formerly MIL-S-5000) heat treated to 260 – 280 ksi (pounds per square inch x 1000) as the baseline. This combination of alloy and heat treat level has been used for many years and a large database has been accumulated in the aerospace industry on its specific response to exposure...

  8. Lithospheric deformation in the Africa-Iberia plate boundary: Improved neotectonic modeling testing a basal-driven Alboran plate

    Science.gov (United States)

    Neres, M.; Carafa, M. M. C.; Fernandes, R. M. S.; Matias, L.; Duarte, J. C.; Barba, S.; Terrinha, P.

    2016-09-01

    We present an improved neotectonic numerical model of the complex NW Africa-SW Eurasia plate boundary segment that runs from west to east along the Gloria Fault up to the northern Algerian margin. We model the surface velocity field and the ongoing lithospheric deformation using the most recent version of the thin-shell code SHELLS and updated lithospheric model and fault map of the region. To check the presence versus the absence of an independently driven Alboran domain, we develop two alternative plate models: one does not include an Alboran plate; another includes it and determines the basal shear tractions necessary to drive it with known velocities. We also compare two alternative sets of Africa-Eurasia velocity boundary conditions, corresponding to geodetic and geological-scale averages of plate motion. Finally, we perform an extensive parametric study of fault friction coefficient, trench resistance, and velocities imposed in Alboran nodes. The final run comprises 5240 experiments, each scored to geodetic velocities (estimated for 250 stations and here provided), stress direction data, and seismic strain rates. The model with the least discrepancy to the data includes the Alboran plate driven by a basal WSW directed shear traction, slightly oblique to the westward direction of Alboran motion. We provide estimates of long-term strain rates and slip rates for the modeled faults, which can be useful for further hazard studies. Our results support that a mechanism additional to the Africa-Eurasia convergence is required to drive the Alboran domain, which can be related to subduction processes occurring within the mantle.

  9. Reliability and Lifetime Prediction of Remote Phosphor Plates in Solid-State Lighting Applications Using Accelerated Degradation Testing

    NARCIS (Netherlands)

    Yazdan Mehr, M.; Van Driel, W.D.; Zhang, G.Q.

    2015-01-01

    A methodology, based on accelerated degradation testing, is developed to predict the lifetime of remote phosphor plates used in solid-state lighting (SSL) applications. Both thermal stress and light intensity are used to accelerate degradation reaction in remote phosphor plates. A reliability model,

  10. Can ultrasound of plantar plate have normal appearance with a positive drawer test?

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, Eloy de Avila [Affiliated Professor, Department of Diagnostic Imaging, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM-Unifesp), São Paulo (Brazil); Mann, Tania Szejnfeld [Medical Assistant of Medicine and Surgery of the Foot and Ankle Group, Department of Orthopedics and Traumatology, EPM-Unifesp, São Paulo (Brazil); Puchnick, Andrea, E-mail: andrea.ddi@epm.br [Professor and Coordinator of Educational and Research Support, Department of Diagnostic Imaging, EPM-Unifesp, São Paulo (Brazil); Tertulino, Franklin de Freitas [Postgraduate Physician, Department of Diagnostic Imaging, EPM-Unifesp, São Paulo (Brazil); Cannato, Camila Testoni [Resident Physician, Department of Surgery, EPM-Unifesp, São Paulo (Brazil); Nery, Caio [Associate Professor, Department of Orthopedics and Traumatology, EPM-Unifesp, São Paulo (Brazil); Fernandes, Artur da Rocha Corrêa [Associate Professor, Department of Diagnostic Imaging, EPM-Unifesp, São Paulo (Brazil)

    2015-03-15

    Highlights: •We evaluate the accuracy of ultrasound to identify and measure the plantar plate. •We correlate ultrasound findings with those of physical examination and MRI. •Ultrasound and MRI measures of plantar plate were positively correlated. •Ultrasound is efficient in identifying and measuring plantar plate. •Ultrasound may complement physical examination. •Young asymptomatic subjects can present a grade I positive drawer test. -- Abstract: Objectives: The aims of this study were (1) to evaluate the reliability of ultrasound (US) examination in the identification and measurement of the metatarsophalangeal plantar plate (MTP-PP) in asymptomatic subjects and (2) to establish the correlation of US findings with those of physical examination and magnetic resonance imaging (MRI), once it is an important tool in the evaluation of the instability syndrome of the second and third rays. Materials and Methods: US examinations of the second and third MTP-PPs were performed in eight asymptomatic volunteers, totaling 32 MTP joints, by three examiners with different levels of experience in musculoskeletal US. Plantar plate dimensions, integrity and echogenicity, the presence of ruptures, and confidence level in terms of structure identification were determined using conventional US. Vascular flow was assessed using power Doppler. US data were correlated with data from physical examination and MRI. Results: MTP-PPs were ultrasonographically identified in 100% of cases, always showing homogeneous hyperechoic features and no detectable vascular flow on power Doppler, with 100% certainty in identification for all examiners. There was excellent US inter-observer agreement for longitudinal measures of second and third toe MTP-PPs and for transverse measures of the second toe MTP-PP. The MTP drawer test was positive for grade 1 MTP instability in 34.4% of joints with normal US results. Transverse MTP-PP measures were significantly higher in individuals with positive

  11. A test of present-day plate geometries for northeast Asia and Japan

    Science.gov (United States)

    Demets, Charles

    1992-01-01

    Alternative geometries for the present-day configuration of plate boundaries in northeast Asia and Japan are tested using NUVEL-1 and 256 horizontal earthquake slip vectors from the Japan and northern Kuril trenches. Statistical analysis of the slip vectors is used to determine whether the North American, Eurasian, or Okhotsk plate overlies the trench. Along the northern Kuril trench, slip vectors are well-fit by the NUVEL-1 Pacific-North America Euler pole, but are poorly fit by the Pacific-Eurasia Euler pole. Results for the Japan trench are less conclusive, but suggest that much of Honshu and Hokkaido are also part of the North American plate. The simplest geometry consistent with the trench slip vectors is a geometry in which the North American plate extends south to 41 deg N, and possibly includes northern Honshu and southern Hokkaido. Although these results imply that the diffuse seismicity that connects the Lena River delta to Sakhalin Island and the eastern Sea of Japan records motion between Eurasia and North America, onshore geologic and seismic data define an additional belt of seismicity in Siberia that cannot be explained with this geometry. Assuming that these two seismic belts constitute evidence for an Okhotsk block, two published kinematic models for motion of the Okhotsk block are tested. The first model, which predicts motion of up to 15 mm/yr relative to North America, is rejected because Kuril and Japan trench slip vectors are fit more poorly than for the simpler geometry described above. The second model gives a good fit to the trench slip vectors, but only if Okhotsk-North America motion is slower than 5 mm/yr.

  12. Deliverable 4.6: Data of the guided ditching tests for permanent deformation (aluminium and composite plates)

    OpenAIRE

    Iafrati, Alessandro; Olivieri, Flavio; Santos, Victor Hugo; Grizzi, Silvano; Fortunati, Marco; Ortolani, Fabrizio; Mauro, Luca

    2014-01-01

    In the present report the data of the guided ditching tests of the plates which were expected to undergo permanent deformations are presented and discussed briefly. Before going into the details of the data, some aspects of the experimental setup which were specific for tests on the deformable plates are provided. The last section is devoted to the presentation of the data of the DASSAULT specimen tested at the high speed ditching facility.

  13. Enhancement of Leptospira hardjo agglutination titers in sheep and goat serum by heat inactivation.

    Science.gov (United States)

    Malkin, K

    1984-04-01

    Heat inactivation of sheep serum samples resulted in the detection of an additional 9% reactors to Leptospira hardjo that were negative on the initial test of fresh samples. Treatment with EDTA gave results generally similar to heat inactivation suggesting that complement was responsible for the inhibition of agglutination. Tests on heat inactivated serum from experimentally infected sheep and goats revealed enhanced titers or reactions which were not detected in fresh serum.

  14. Pilot Experimental Tests on Punching Shear Strength of Flat Plates Reinforced with Stirrups Punching Shear Reinforcement

    Directory of Open Access Journals (Sweden)

    Mohamed Hassan

    2017-03-01

    Full Text Available Flat plates are favor structure systems usually used in parking garages and high-rise buildings due to its simplicity for construction. However, flat plates have some inherent structural problems, due to high shear stress surrounding the supporting columns which cause a catastrophic brittle type of failure called "Punching Shear Failure". Several solutions are used to avoid punching shear failure, including the use of drop panels or punching shear reinforcement. The latter is being a more sophisticated solution from the structural ductility, the architectural and the economical point of view. This study aims at investigating the effect of stirrups as shear reinforcement in enhancing the punching strength of interior slab-column connections. A total of four full-scale interior slab-column connections were tested up to failure. All slabs had a side length of 1700 mm and 160 mm thickness with 200 mm x 200 mm square column. The test parameters were the presence of shear reinforcement and stirrups concentration around the supporting column. The test results showed that the distribution of stirrups over the critical punching shear zone was an efficient solution to enhance not only the punching shear capacity but also the ductility of the connection. Furthermore, the concentrating of stirrups shear reinforcement in the vicinity of the column for the tested slabs increases the punching shear capacity by 13 % compared to the uniform distribution at same amount of shear reinforcement.

  15. Uncertainty of pin height measurement for the determination of wear in pin-on-plate test

    DEFF Research Database (Denmark)

    Drago, Nicola; De Chiffre, Leonardo; Poulios, Konstantinos

    2014-01-01

    machine (CMM), achieving an expanded measurement uncertainty (k = 2) better than 1 mm. A simple dedicated fixture adaptable to workshop environment was developed and its metrological capability investigated, estimating an average uncertainty of measurement in the order of 5 mm (k = 2). Fixture......The paper concerns measurement of pin height for the determination of wear in a pin-on-plate (POP) or pin-on-disc (POD) test, where a pin is mounted on a holder that can be fixed on the test rig and removed for measurements. The amount of wear is assessed as difference of pin height before...... and after the test, using the distance between holder plane and pin friction plane as measurand. A series of measurements were performed in connection with POP testing of different friction material pins mounted on an aluminium holder. Pin height measurements were carried out on a coordinate measuring...

  16. Evaluation of agglutination strength by a flow-induced cell movement assay based surface plasmon resonance (SPR) technique.

    Science.gov (United States)

    Sudprasert, Krisda; Peungthum, Patjaree; Vongsakulyanon, Apirom; Amarit, Ratthasart; Somboonkaew, Armote; Sutapun, Boonsong; Kitpoka, Pimpun; Kunakorn, Mongkol; Srikhirin, Toemsak

    2015-02-07

    A flow-induced cell movement assay combined with a surface plasmon resonance (SPR) technique was developed to quantify the agglutination strength, derived from the standard tube-agglutination test. Red blood cells (RBCs), based on the ABO blood group system, were specifically captured by anti-A and/or anti-B antibodies immobilized on a sensor surface. The agglutination strength corresponds to the amount of antigen-antibody interactions or the strength of RBC adhesion. Under a shear flow, the adherent RBCs were forced to move out of the region of interest with different average cell velocities (vc) depending upon the adhesion strength and wall shear stress (WSS). That is, a higher adhesion strength (higher agglutination strength) or lower WSS represents a lower vc or vice versa. In this work, the agglutination strength was derived from the vc that was calculated from the time derivative of the relative SPR signal by using a simple model of cell movement response, whose validity was verified. The vc values of different samples were correlated with their agglutination strengths at a given WSS and antibody surface density. The vc decreased as the agglutination strength increased, which can be considered as a linear regression. The coefficient of variation of the calculated vc decreased to 0.1 as vc increased to 30 μm min(-1). The sensitivity of this assay can be controlled by optimizing the antibody surface density or the WSS. This assay has the capability to resolve the antigen density of A1 and B RBCs from that of A1B RBCs.

  17. Serotype assignment by sero-agglutination, ELISA, and PCR

    Science.gov (United States)

    For assessing isolates of Listeria monocytogenes serotype designation is the foremost subtyping method used. Traditionally serotyping has been done with agglutination reactions. In the last decade alternative serotyping methods were described using Enzyme Linked Immunosorbent Assay(ELISA)and Polymer...

  18. Surgical management of vulvovaginal agglutination due to lichen planus.

    Science.gov (United States)

    Fairchild, Pamela S; Haefner, Hope K

    2016-02-01

    Lichen planus is a rare dermatological disorder that is often associated with painful and disfiguring vulvovaginal effects. At the University of Michigan Center for Vulvar Diseases, we see many women with vulvovaginal lichen planus each year, with marked scarring and vulvovaginal agglutination that precludes vaginal intercourse and causes difficulty with urination. Through our experience, we developed a protocol for the operative management and postoperative care for severe vulvovaginal agglutination. Our objective is to share this protocol with a wider audience so that providers who see patients with these devastating effects of lichen planus can benefit from our experience to better serve this patient population. The figure represents a case of erosive lichen planus with early vaginal agglutination. The video reviews the pathophysiology and presentation of lichen planus. We then present a case of scarring and agglutination in a young woman, including our surgical management and postoperative care recommendations.

  19. Test and characterization of multigap resistive plate chambers for the EEE project

    CERN Document Server

    Bossini, E

    2016-01-01

    The Extreme Energy Events project is based on the deployment of cosmic-ray telescopes in Italian high schools with the active contribution of students and teachers. Each telescope is made by three Multigap Resistive Plate Chambers readout by strips. With around 50 telescopes already built and others under construction, specific systems to test and characterize the chambers are needed. In this article I will present a flexible and software-configurable solution to perform chamber efficiency studies with a set of scintillators and hardware to automatically scan detector strips to identify electrical issues. Both systems can provide accurate information but at the same time they can be easily operated by students.

  20. Test and characterization of multigap resistive plate chambers for the EEE project

    Science.gov (United States)

    Bossini, E.; EEE Collaboration

    2016-03-01

    The Extreme Energy Events project is based on the deployment of cosmic-ray telescopes in Italian high schools with the active contribution of students and teachers. Each telescope is made by three Multigap Resistive Plate Chambers readout by strips. With around 50 telescopes already built and others under construction, specific systems to test and characterize the chambers are needed. In this article I will present a flexible and software-configurable solution to perform chamber efficiency studies with a set of scintillators and hardware to automatically scan detector strips to identify electrical issues. Both systems can provide accurate information but at the same time they can be easily operated by students.

  1. Detection of Legionella antigenuria by reverse passive agglutination.

    OpenAIRE

    Tang, P W; Savigny, D. de; Toma, S

    1982-01-01

    A reverse passive agglutination method was developed to detect soluble antigens of Legionella spp. By this method Legionella antigens were detected in urine specimens from 14 of 15 antigenuric patients with clinically diagnosed Legionnaires disease and in none of 263 urine samples from healthy subjects or patients with urinary tract infections. Intra-genus cross-reactivity was observed only between L. pneumophila serogroups 2, 3, and 6. The Legionella reverse passive agglutination method was ...

  2. Improved Thermal-Vacuum Compatible Flat Plate Radiometric Souce for System-Level Testing of Optical Sensors

    Science.gov (United States)

    Schwarz, Mark A.; Kent, Craig J.; Bousquet, Robert; Brown, Steven W.

    2015-01-01

    This work describes the development of an improved vacuum compatible flat plate radiometric source used for characterizing and calibrating remote optical sensors, in situ, throughout their testing period. The original flat plate radiometric source was developed for use by the VIIRS instrument during the NPOESS Preparatory Project (NPP). Following this effort, the FPI has had significant upgrades in order to improve both the radiometric throughput and uniformity. Results of the VIIRS testing with the reconfigured FPI are reported and discussed.

  3. Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test Detecção de antígenos bacterianos no líquido cefalorraquidiano através do teste de aglutinação de latex

    Directory of Open Access Journals (Sweden)

    Ilka Maria Landgraf

    1995-06-01

    Full Text Available Eighty purulent cerebrospinal fluid (CSF samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE, as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C.Oitenta amostras purulentas de líquido cefalorraquidiano (LCR de pacientes com evidência clínica de meningite foram estudadas empregando-se Kit Directigen de aglutinação de latex (AL para demonstrar antígeno bacteriano no LCR. Os resultados mostraram que o teste de AL apresentou melhor desempenho diagnóstico do que bacterioscopia através da coloração de Gram, cultura e imunoeletroforese cruzada (IEC em relação à Neisseria meningitidis grupos B e C, e ao Haemophilus influenzae tipo b, e melhor do que coloração de Gram e cultura quando Streptococcus pneumoniae foi avaliado. A comparação dos resultados com os de cultura mostrou o maior nível de sensibilidade considerando-se N. meningitidis grupo C. Quanto à especificidade, os valores foram satisfatórios para todos os microrganismos testados. O grau de concordância K em relação à IEC exibiu melhores índices K de concordância para N. meningitidis grupos B e C.

  4. Characterization tests of a new parallel plate ionization chamber for use in electron beams

    Science.gov (United States)

    Nonato, Fernanda B. C.; Sakuraba, Roberto K.; da Cruz, José Carlos; Caldas, Linda V. E.

    2014-11-01

    Linear accelerators with electron beams are used in several Brazilian hospitals. Consequently, there is an increasing demand for parallel-plate ionization chambers, to be utilized for dosimetry of electron beams. In Brazil, the commercial ionization chambers utilized are imported. The ionization chambers have usually a simple construction, using different materials and geometries. A homemade ionization chamber was developed to be used in electron beams of linear accelerator. The ionization chamber body is made of acrylic and the collecting electrode is painted with graphite powder mixed with nail polish. Several tests were applied, and the results showed values better than the limits established by the international recommendations, except for the polarity effect test, but the response of the developed ionization chamber, for this test, is similar in relation to the response of other commercial ionization chambers from the literature.

  5. Antibody blocks acquisition of bacterial colonization through agglutination.

    Science.gov (United States)

    Roche, A M; Richard, A L; Rahkola, J T; Janoff, E N; Weiser, J N

    2015-01-01

    Invasive infection often begins with asymptomatic colonization of mucosal surfaces. A murine model of bacterial colonization with Streptococcus pneumoniae was used to study the mechanism for mucosal protection by immunoglobulin. In previously colonized immune mice, bacteria were rapidly sequestered within large aggregates in the nasal lumen. To further examine the role of bacterial agglutination in protection by specific antibodies, mice were passively immunized with immunoglobulin G (IgG) purified from antipneumococcal sera or pneumococcal type-specific monoclonal human IgA (hIgA1 or hIgA2). Systemically delivered IgG accessed the mucosal surface and blocked acquisition of colonization and transmission between littermates. Optimal protection by IgG was independent of Fc fragment and complement and, therefore, did not involve an opsonophagocytic mechanism. Enzymatic digestion or reduction of IgG before administration showed that protection required divalent binding that maintained its agglutinating effect. Divalent hIgA1 is cleaved by the pneumococcal member of a family of bacterial proteases that generate monovalent Fabα fragments. Thus, passive immunization with hIgA1 blocked colonization by an IgA1-protease-deficient mutant (agglutinated) but not the protease-producing wild-type parent (not agglutinated), whereas protease-resistant hIgA2 agglutinated and blocked colonization by both. Our findings highlight the importance of agglutinating antibodies in mucosal defense and reveal how successful pathogens evade this effect.

  6. Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei.

    Science.gov (United States)

    Duval, Brea D; Elrod, Mindy G; Gee, Jay E; Chantratita, Narisara; Tandhavanant, Sarunporn; Limmathurotsakul, Direk; Hoffmaster, Alex R

    2014-06-01

    Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.

  7. Agglutination of human erythrocytes by the interaction of Zn(2+)ion with histidine-651 on the extracellular domain of band 3.

    Science.gov (United States)

    Kiyotake, Kento; Ochiai, Hideharu; Yamaguchi, Takeo

    2016-05-01

    Clustering of band 3, chloride/bicarbonate exchanger, has been reported in Zn(2+)-treated human erythrocytes. However, the agglutination of human erythrocytes is also induced by the interaction of Zn(2+)ion with histidine on band 3. Identification of histidine that interacts with Zn(2+)ion remains to be determined. The Zn(2+)-induced agglutination of human erythrocytes was unaffected by chymotrypsin cleavage of the small loop region containing His-547 in the extracellular domain of band 3. On the other hand, papain digestion of the large loop region containing His-651 in band 3 inhibited such Zn(2+)-induced agglutination. Moreover, Zn(2+)-induced erythrocyte agglutination was inhibited by the peptide (ARGWVIHPLG) containing His-651, but not by the peptide such as ARGWVIRPLG, which His-651 was substituted by arginine. Among 10 kinds of animal erythrocytes tested, interestingly, no agglutination by Zn(2+)ions was observed in cow cells only that the forth amino acid in the upstream from His-669 on the large loop of cow band 3 is aspartate (Asp-665) instead of glycine. As expected, the agglutination of human erythrocytes by Zn(2+) ions was inhibited in the presence of aspartate. These data indicate that the interaction of Zn(2+) ion with His-651 residue of band 3 plays an important role in the Zn(2+)-induced agglutination of human erythrocytes.

  8. Fabrication and testing of U-7Mo monolithic plate fuel with Zircaloy cladding

    Science.gov (United States)

    Pasqualini, E. E.; Robinson, A. B.; Porter, D. L.; Wachs, D. M.; Finlay, M. R.

    2016-10-01

    Nuclear fuel designs are being developed to replace highly enriched fuel used in research and test reactors with fuels of low enrichment. In the most challenging cases, U-(7-10 wt%)Mo monolithic plate fuels are proposed. One of the considered designs includes aluminum-alloy cladding, which provides some challenges in fabrication and fuel/cladding interaction during service. Zircaloy cladding, specifically Zry-4, was investigated as an alternative cladding, and development of a fabrication method was performed by researchers with the Comisión Nacionalde Energia Atómica (CNEA) in Argentina, resulting in test fuel plates (Zry-4 clad U-7Mo) which were subsequently tested in the Advanced Test Reactor in Idaho. Because Zry-4 and U-(7-10)Mo have similar high-temperature mechanical properties, fabrication was simplified in that the fuel foil and cladding could be co-rolled and bonded. The challenge was to prevent a thermal-expansion mismatch, which could destroy the fuel/cladding bond before complete bonding was achieved; the solution was to prevent the composites from cooling significantly during or between roll passes. The final product performed very well in-reactor, showing good bonding, very little fuel/cladding interaction-either from fabrication or in-reactor testing-and little swelling, especially no detectable heterogeneous bubble formation at the fuel/cladding interface tested to a fission density of up to 2.7E+21 (average) fissions/cm3, 3.8E+21 (peak).

  9. Determining the fibrillar orientation of bast fibres with polarized light microscopy: the modified Herzog test (red plate test) explained.

    Science.gov (United States)

    Haugan, E; Holst, B

    2013-11-01

    The identification of bast fibre samples, in particular, bast fibres used in textiles, is an important issue in archaeology, criminology and other scientific fields. One of the characteristic features of bast fibres is their fibrillar orientation, referred to as Z- or S twist (or alternatively right- and left-handed fibres). An empirical test for determining the fibrillar orientation using polarized light microscopy has been known in the community for many years. It is referred to as the modified Herzog test or red plate test. The test has the reputation for never producing false results, but also for occasionally not working. However, so far, no proper justification has been provided in the literature that the 'no false results' assumption is really correct and it has also not been clear up till now, why the method sometimes does not work. In this paper, we present an analytical model for the modified Herzog test, which explains why the test never gives a false result. We also provide an explanation for why the Herzog test sometimes does not work: According to our model, the Herzog test will not work if none of the three distinct layers in the secondary cell wall is significantly thicker than the others.

  10. Determining the fibrillar orientation of bast fibres with polarized light microscopy: the modified Herzog test (red plate test) explained

    Science.gov (United States)

    HAUGAN, E; HOLST, B

    2013-01-01

    The identification of bast fibre samples, in particular, bast fibres used in textiles, is an important issue in archaeology, criminology and other scientific fields. One of the characteristic features of bast fibres is their fibrillar orientation, referred to as Z- or S twist (or alternatively right- and left-handed fibres). An empirical test for determining the fibrillar orientation using polarized light microscopy has been known in the community for many years. It is referred to as the modified Herzog test or red plate test. The test has the reputation for never producing false results, but also for occasionally not working. However, so far, no proper justification has been provided in the literature that the ‘no false results’ assumption is really correct and it has also not been clear up till now, why the method sometimes does not work. In this paper, we present an analytical model for the modified Herzog test, which explains why the test never gives a false result. We also provide an explanation for why the Herzog test sometimes does not work: According to our model, the Herzog test will not work if none of the three distinct layers in the secondary cell wall is significantly thicker than the others. PMID:24020614

  11. Design, construction and testing of a liquid-heating flat-plate solar collector

    Energy Technology Data Exchange (ETDEWEB)

    Tuttle, R E

    1980-02-01

    The purpose of this study was to design, construct, and test a liquid-heating flat-plate solar collector. From the literature search, information was gathered concerning the basic components of the collector, the different processes and materials that can be used in the construction of these components, and their advantages and disadvantages. The literature search also revealed a method used to measure the performance of the collector in terms of efficiency and heat output. Design considerations were then listed for each of the major components as well as the collector as a single unit. Then, each component was designed, taking into consideration the final assembly of the completed unit. Detailed designs were required for the absorber plate and the box and frame assembly because of their complexity in construction and assembly. Once the components were designed, the construction details were arranged in a logical sequence, again considering the final assembly of the unit. The collector was then carefully constructed and assembled following the design details. After the solar collector was assembled completely, tests were made, data were obtained and recorded, and a collector performance curve was developed.

  12. An Intelligent Tap Test as AN Inspection Tool for Corrosion in Chequer Plate Floors

    Science.gov (United States)

    Williams, S. M.; Smith, J. W.

    2002-11-01

    The "coin-tap" test has the ability to indicate damage in a structural element due to a localized change of stiffness or damping. The change in vibration signature may be detected by ear or more precisely by measurement of the dynamic contact force. A method for discriminating between measurements made on sound and damaged structures is presented. An unsupervised neural network algorithm is used for recognizing the differences between contact force patterns. The method is used for non-destructive inspection of corrosion damage to steel chequer plate floors in industrial buildings. It is shown that the intelligent tap test is a useful and practical diagnostic tool for detecting localized damage in structures.

  13. [Isolation of an agglutinating anti-E. coli K 88+ serum].

    Science.gov (United States)

    Petkov, M; Belchev, K; Ganovski, D

    1981-01-01

    An agglutinating anti-K 88+ serum was obtained through immunizing rabbits with geometrically rising amounts of cell-free K 88 antigenic extraction. Use was made of bacterial suspensions cultured in Minka agar and homogenized at 8000 r. p. m. to remove the K 88 pili. The cell depot was removed by centrifugation at 15 000 r. p. m., and the protein in the supernatant was determined by the method of Kingsey. The titer of the K88 serum was within the 1:320-1:640 range. The specificity and activity of the serum was evaluated by the hemagglutination test, immunoelectrophoresis, and immunodiffusion. The serum is highly specific and yields positive agglutination results with all K 88+ Escherichia coli strains. It does not react with antigen - K 88-negative E. coli organisms as well as with the O antigen of the investigated strains.

  14. Evaluation of protective ensemble thermal characteristics through sweating hot plate, sweating thermal manikin, and human tests.

    Science.gov (United States)

    Kim, Jung-Hyun; Powell, Jeffery B; Roberge, Raymond J; Shepherd, Angie; Coca, Aitor

    2014-01-01

    The purpose of this study was to evaluate the predictive capability of fabric Total Heat Loss (THL) values on thermal stress that Personal Protective Equipment (PPE) ensemble wearers may encounter while performing work. A series of three tests, consisting of the Sweating Hot Plate (SHP) test on two sample fabrics and the Sweating Thermal Manikin (STM) and human performance tests on two single-layer encapsulating ensembles (fabric/ensemble A = low THL and B = high THL), was conducted to compare THL values between SHP and STM methods along with human thermophysiological responses to wearing the ensembles. In human testing, ten male subjects performed a treadmill exercise at 4.8 km and 3% incline for 60 min in two environmental conditions (mild = 22°C, 50% relative humidity (RH) and hot/humid = 35°C, 65% RH). The thermal and evaporative resistances were significantly higher on a fabric level as measured in the SHP test than on the ensemble level as measured in the STM test. Consequently the THL values were also significantly different for both fabric types (SHP vs. STM: 191.3 vs. 81.5 W/m(2) in fabric/ensemble A, and 909.3 vs. 149.9 W/m(2) in fabric/ensemble B (p values are significantly different from the actual THL potential of the PPE ensemble tested on STM, (2) physiological benefits from wearing a more breathable PPE ensemble may not be feasible with incremental THL values (SHP test) less than approximately 150-200 W·m(2), and (3) the effects of thermal environments on a level of heat stress in PPE ensemble wearers are greater than ensemble thermal characteristics.

  15. R and G color component competition of RGB image decomposition as a criterion to register RBC agglutinates for blood group typing.

    Science.gov (United States)

    Doubrovski, Valeri A; Ganilova, Yuliya A; Zabenkov, Igor V

    2014-03-01

    A new approach of the criterion assignment for registration of erythrocyte agglutinates to instrumentally determine blood group type is suggested. The criterion is based on comparison of R and G components of RGB decomposition of microscopy digital image taken for the blood-serum mixture sample. For the chosen experimental conditions, the minimal size (area) of RBC agglutinate to be registered by the criterion suggested is estimated theoretically. The proposed method was tested experimentally on the example of monitoring agglutinates in flow. The encouraging experimental results were obtained for improvement of the resolving power of the method; the optimal experimental conditions were revealed for maximum resolution. Though the suggested method was realized for dynamic (flow) blood group determination, it could also be applied for diagnostics in a stationary environment. This approach increases the reliability of RBC agglutinates registration and, hence, blood group typing. The results may be used to develop the apparatus for automated determination of human blood group.

  16. Direct tests of a pixelated microchannel plate as the active element of a shower maximum detector

    Science.gov (United States)

    Apresyan, A.; Los, S.; Pena, C.; Presutti, F.; Ronzhin, A.; Spiropulu, M.; Xie, S.

    2016-08-01

    One possibility to make a fast and radiation resistant shower maximum detector is to use a secondary emitter as an active element. We report our studies of microchannel plate photomultipliers (MCPs) as the active element of a shower-maximum detector. We present test beam results obtained using Photonis XP85011 to detect secondary particles of an electromagnetic shower. We focus on the use of the multiple pixels on the Photonis MCP in order to find a transverse two-dimensional shower distribution. A spatial resolution of 0.8 mm was obtained with an 8 GeV electron beam. A method for measuring the arrival time resolution for electromagnetic showers is presented, and we show that time resolution better than 40 ps can be achieved.

  17. Testing micro-channel plate detectors for the particle identification upgrade of LHCb

    CERN Document Server

    Castillo Garcia, L

    2012-01-01

    The TORCH, Time of internally Reflected Cherenkov Light, is proposed for the high luminosity upgrade of the LHCb experiment. The detector combines Time-of-Flight and Cherenkov techniques to achieve positive pi/K/p separation on a >= 3 sigma level in the momentum range below 10 GeV/c. The required time resolution is <= 50 ps for single photon signal. In a preliminary R\\&D phase, we have shown that already commercially available micro-channel plate tubes with 8 x 8 channels fulfil the requirements. Timing properties of the tubes have been investigated with a pulsed laser diode in single photon regime. Key results from these laboratory tests are reported. An excellent timing resolution of <40 ps is achieved with an efficiency of similar to 90\\%. (C) 2011 Elsevier B.V. All rights reserved.

  18. Direct tests of a pixelated microchannel plate as the active element of a shower maximum detector

    Energy Technology Data Exchange (ETDEWEB)

    Apresyan, A. [California Institute of Technology, Pasadena, CA (United States); Los, S. [Fermi National Accelerator Laboratory, Batavia, IL (United States); Pena, C.; Presutti, F. [California Institute of Technology, Pasadena, CA (United States); Ronzhin, A. [Fermi National Accelerator Laboratory, Batavia, IL (United States); Spiropulu, M.; Xie, S. [California Institute of Technology, Pasadena, CA (United States)

    2016-08-21

    One possibility to make a fast and radiation resistant shower maximum detector is to use a secondary emitter as an active element. We report our studies of microchannel plate photomultipliers (MCPs) as the active element of a shower-maximum detector. We present test beam results obtained using Photonis XP85011 to detect secondary particles of an electromagnetic shower. We focus on the use of the multiple pixels on the Photonis MCP in order to find a transverse two-dimensional shower distribution. A spatial resolution of 0.8 mm was obtained with an 8 GeV electron beam. A method for measuring the arrival time resolution for electromagnetic showers is presented, and we show that time resolution better than 40 ps can be achieved.

  19. Conclusions and recommendations for the testing of flat-plate solar collector thermal performance and durability

    Science.gov (United States)

    Waksman, D.; Thomas, W. C.

    1984-12-01

    The results of studies, by the National Bureau of Standards, of the reliability and durability of eight different types of flat plate solar collectors representative of equipment available in 1977 are reported. The installations were made in four sites believed to typify various U.S. climates. The stability of the thermal performance and material properties was tracked, and measured again after moving the units inside for exposure to artificial sunlight. The stagnation measurement techniques employed to evaluate the collectors were judged adequate, provided the tests are made on-site and out of doors. It is noted that the instrumentation used to gather sufficient data for valid analyses may experience performance decrements due to the necessarily long monitoring intervals, i.e., several years.

  20. Detection of Salmonella enterica serovar Enteritidis (SE) Antibodies in Serum Using A Polystyrene Bead/SE Flagella Agglutination Assay

    Science.gov (United States)

    Serologic screening of flocks can be an important method to detect Salmonella enteritidis (SE) infections but can be labor intensive or lack specificity. Our goal was to develop a rapid agglutination assay using SE flagella adsorbed to polystyrene beads as a simple, relatively specific test to dete...

  1. Use of commercial extenders and alternatives to prevent sperm agglutination for cryopreservation of brown bear semen.

    Science.gov (United States)

    Gomes-Alves, S; Alvarez, M; Nicolas, M; Lopez-Urueña, E; Martínez-Rodríguez, C; Borragan, S; de Paz, P; Anel, L

    2014-08-01

    The objective of this study was to evaluate different bovine and canine commercial semen extenders for cryopreservation of brown bear ejaculates and the effect of semen collection directly into extender on sperm agglutination. Semen samples were obtained by electroejaculation from 13 adult males. In experiment 1, eleven ejaculates from eight bears were used to evaluate Bioxcell and Andromed as extenders, whereas in experiment 2, nine ejaculates from six bears were used to evaluate Triladyl canine, CaniPro, and Extender 2 as extenders. An extender specifically developed for brown bears (Test-Tris-fructose-egg yolk-glycerol, TTF-ULE/bear) served as a control extender in both experiments. After thawing, total and progressive sperm motility and sperm viability were greater (P agglutination in fresh samples (score 0.5 ± 0.2 vs. 1.8 ± 0.4 in diluted and control samples, respectively) with no effect on pre-freeze and post-thawing semen quality. In conclusion, TTF-ULE/bear is the most suitable extender for brown bear semen cryopreservation, but comparable results can be obtained with the commercial extender Andromed. In addition, collection of ejaculates directly in TTF-H extender decreases sperm agglutination in fresh samples.

  2. Lack of chemical fractionation in major and minor elements during agglutinate formation. [in lunar soil

    Science.gov (United States)

    Hu, H.-N.; Taylor, L. A.

    1977-01-01

    Rhodes et al. (1975, 1976) and Adams et al. (1975) have reported that the agglutinate fraction of the soils on the lunar surface displays a marked enrichment in Fe, Mg, Ti, K, and La, and a depletion in Ca, Na, Al, and Eu, relative to the bulk soils. The reported investigation is concerned with a testing of the theory of chemical fractionation involving magnetic separation which was developed in connection with these findings. Soils 64421 and 71501 were sieved and the magnetic fractions separated according to the method developed by Adams and McCord (1973). Analyses of agglutinitic glass did not indicate any appreciable chemical fractionation for the major and minor elements accompanying the agglutination process. It was found that most, if not all fractionations reported can be accounted for completely by the magnetic nonagglutinate impurities in the agglutinate fraction. It is, therefore, concluded that there appears to be no reason to make use of any chemical fractionation theory, whose validity remains to be demonstrated.

  3. Standardized performance tests of collectors of solar thermal energy - A flat-plate copper collector with parallel mylar striping

    Science.gov (United States)

    Johnson, S. M.

    1976-01-01

    Basic test results are reported for a flat plate solar collector whose performance was determined in a solar simulator. The collector was tested over ranges of inlet temperatures, fluxes and one coolant flow rate. Collector efficiency is correlated in terms of inlet temperature and flux level.

  4. A pulse-shaping technique to investigate the behaviour of brittle materials subjected to plate-impact tests

    Science.gov (United States)

    Forquin, Pascal; Zinszner, Jean-Luc

    2017-01-01

    Owing to their significant hardness and compressive strengths, ceramic materials are widely employed for use with protective systems subjected to high-velocity impact loadings. Therefore, their mechanical behaviour along with damage mechanisms need to be significantly investigated as a function of loading rates. However, the classical plate-impact testing procedures produce shock loadings in the brittle sample material which cause unrealistic levels of loading rates. Additionally, high-pulsed power techniques and/or functionally graded materials used as flyer plates to smooth the loading pulse remain costly, and are generally difficult to implement. In this study, a shockless plate-impact technique based on the use of either a wavy-machined flyer plate or buffer plate that can be produced by chip-forming is proposed. A series of numerical simulations using an explicit transient dynamic finite-element code have been performed to design and validate the experimental testing configuration. The calculations, conducted in two-dimensional (2D) plane-strain or in 2D axisymmetric modes, prove that the `wavy' contact surface will produce a pulse-shaping effect, whereas the buffer plate will produce a homogenizing effect of the stress field along the transverse direction of the sample. In addition, `wavy-shape' geometries of different sizes provide an easy way to change the level of loading rate and rise time in an experimentally tested ceramic specimen. Finally, when a shockless compression loading method is applied to the sample, a Lagrangian analysis of data is made possible by considering an assemblage of ceramic plates of different thicknesses in the target, so the axial stress-strain response of the brittle sample material can be provided. This article is part of the themed issue 'Experimental testing and modelling of brittle materials at high strain rates'.

  5. Solar tests of aperture plate materials for solar thermal dish collectors

    Science.gov (United States)

    Jaffe, L. D.

    1984-11-01

    If a malfunction occurs in a solar thermal point-focus distributed receiver power plant while a concentrator is pointed at the sun, motion of the concentrator may stop. As the sun moves relative to the earth, the spot of concentrated sunlight then slowly walks off the receiver aperture, across the receiver face plate, and perhaps across adjacent portions of the concentrator. Intense local heating by the concentrated sunlight may damage or destroy these parts. The behavior of various materials under conditions simulating walk-off of a parabolic dish solar collector were evaluated. Each test consisted of exposure to concentrated sunlight at a peak flux density of about 7000 kW/square meter for 15 minutes. Types of materials tested included graphite, silicon carbide, silica, various silicates, alumina, zirconia, aluminum, copper, steel, and polytetrafluroethylene. The only material that neither cracked nor melted was grade G-90 graphite. Grade CS graphite, a lower cost commercial grade, cracked half-way across, but did not fail apart. Both of these grades are medium-grain extruded graphites. A graphite cloth (graphitized polyacrylonitrile) showed fair performance when tested as a single thin ply; it might be useful as a multi-ply assembly. High purity slipcast silica showed some promise also.

  6. Evaluation of Electroless-Nickel Plated Polypropylene under Thermal Cycling and Mechanical Tests

    Directory of Open Access Journals (Sweden)

    O.O. Ajibola

    2016-09-01

    Full Text Available The electroless-nickel composite (ENC consisting of bright metallic electroless-nickel (EN and dull electroless-nickel-phosphorus (EN-P were deposited on the polypropylene (PP substrate from the sodium hypophosphite baths. The ENC plated specimens were subjected to abrasive wear-adhesion test of 1750, 3500, 7000 and 14000 cycles; thermal cycle-adhesion tests, and tensile strength and creep tests. The deposition of ENC influenced the strength and creep strain properties of the PP. The maximum stress σ of 118 (MPa was obtained from EN-PP specimen at strain  of 0.1 mm/mm as compared with the PP having stress σ of 36 (MPa at strain  of 0.07 mm/mm before failure The surface appearances and microstructures of ENC film on PP substrates were examined under the higher resolution metallurgical microscope with digital camera and microscopic camera. The composition of ENC film was characterized using Scanning Electron Microscopy and Energy Dispersive X-Ray analyses (Jeol JSM-7600F Field Emission SEM/EDX, The micrographs and spectra lines data generated were used to interpret the results.

  7. Bearing Capacity of High Density Polyethylene (HDPE Reinforced Sand Using Plate Load Test

    Directory of Open Access Journals (Sweden)

    Er. Aly K

    2015-06-01

    Full Text Available The work presented here is a study to examine the improvement in bearing capacity of coastal sand of Trivandrum, Kerala, India using high density polyethylene (HDPE /woven fabric as reinforcement in discrete layers. The bearing capacity was evaluated using plate load test. The effect of reinforcement configurations like sheet reinforcement (sanded with adhesive, with adhesive and sheet alone and strip reinforcement (single and grid pattern are investigated. The test parameters chosen for the present study are, depth of topmost layer of reinforcement layer below footing, compacted density and number of layers of reinforcement etc. From the tests, it has been observed that sheet reinforcement is more effective than sheet sanded with adhesive and strip reinforcements. It is found that the synthetic adhesive gives no binding action at the interface of the reinforcement and soil. But it is to be noted that the sheet with adhesive dried has a marked influence on the bearing capacity especially at lower densities. The strip reinforcements in single pattern is considered to be a favorable choice for minimum reinforcement. The strip reinforcement in single or grid pattern gives sufficient improvement in strength.

  8. Solar tests of aperture plate materials for solar thermal dish collectors

    Science.gov (United States)

    Jaffe, L. D.

    1984-01-01

    If a malfunction occurs in a solar thermal point-focus distributed receiver power plant while a concentrator is pointed at the sun, motion of the concentrator may stop. As the sun moves relative to the earth, the spot of concentrated sunlight then slowly walks off the receiver aperture, across the receiver face plate, and perhaps across adjacent portions of the concentrator. Intense local heating by the concentrated sunlight may damage or destroy these parts. The behavior of various materials under conditions simulating walk-off of a parabolic dish solar collector were evaluated. Each test consisted of exposure to concentrated sunlight at a peak flux density of about 7000 kW/square meter for 15 minutes. Types of materials tested included graphite, silicon carbide, silica, various silicates, alumina, zirconia, aluminum, copper, steel, and polytetrafluroethylene. The only material that neither cracked nor melted was grade G-90 graphite. Grade CS graphite, a lower cost commercial grade, cracked half-way across, but did not fail apart. Both of these grades are medium-grain extruded graphites. A graphite cloth (graphitized polyacrylonitrile) showed fair performance when tested as a single thin ply; it might be useful as a multi-ply assembly. High purity slipcast silica showed some promise also.

  9. Evolution of Shock Melt Compositions in Lunar Agglutinates

    Science.gov (United States)

    Vance, A. M.; Christoffersen, R.; Keller, L. P.

    2015-01-01

    Lunar agglutinates are aggregates of regolith grains fused together in a glassy matrix of shock melt produced during smaller-scale (mostly micrometeorite) impacts. Agglutinate formation is a key space weathering process under which the optically-active component of nanophase metallic Fe (npFe(sup 0)) is added to the lunar regolith. Here we have used energy-dispersive X-ray (EDX) compositional spectrum imaging in the SEM to quantify the chemical homogeneity of agglutinitic glass, correlate its homogeneity to its parent soil maturity, and identify the principle chemical components contributing to the shock melt compositional variations.

  10. A Comparative Study of Detection of Bordetella avium Antibodies in Turkeys by ELISA, SPAT, and AGID Test

    OpenAIRE

    TÜRKYILMAZ, Süheyla; TÜRKYILMAZ, Kenan; KAYA, Osman

    2006-01-01

    The aims of this study were to develop a serum plate agglutination test (SPAT) antigen and agar gel immunodiffusion (AGID) test antigen for the serological detection of turkeys that have been exposed to Bordetella avium; to compare the sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with SPAT, and AGID test, and to survey B. avium antibodies in turkey flocks in Aydın, Turkey. For these purposes, serum samples collected from 300 turkeys were examined by ELISA, SPAT, and AGI...

  11. Studies on strains of Pasteurella haemolytica not typable by the indirect haemagglutination test.

    Science.gov (United States)

    Donachie, W; Fraser, J; Quirie, M; Gilmour, N J

    1984-09-01

    Thirty strains of Pasteurella haemolytica which were untypable by the indirect haemagglutination (IHA) test were examined serologically by rapid plate agglutination (RPA), agar gel diffusion (AGD), crossed immunoelectrophoresis (CIE) and counter current immunoelectrophoresis (CCIE) tests. Nine serogroups were identified by CCIE. Serogroup specificity, dependent on two antigens, was present in heated saline extracts of cells. Single representative strains from two serogroups were not pathogenic for specific pathogen-free lambs.

  12. The study of fix composite panel and steel plates on testing stand

    Science.gov (United States)

    Wróbel, A.; Płaczek, M.; Wachna, M.

    2016-08-01

    In this paper the practical possibilities of strength verification analysis of composite materials used in the manufacture of selected components of railway wagons are presented. Real laboratory stand for measurements in a scale controlled by PLC controller were made. The study of different types of connections of composite materials with sheet metal is presented. In one of the chapter of this paper principles construction of testing stand with pneumatic cylinder were presented. Mainly checking of displacements and stresses generated on the sheet as a result of pneumatic actuators load for composite boards was carried out. The use of the controller with operating panel allows to easy programming testing cycle. The user can define the force generated by the actuator by change of air pressure in cylinder. Additionally the location of acting cylinders and their jump can be changed by operator. The examination of the volume displacements was done by displacement sensor, and the tensile strain gauge. All parameters are written in CatmanEasy - data acquisition software. This article presents the study of stresses and displacements in the composite plates joined with sheet metal, in summary of this article, the authors compare the obtained results with the computer simulation results in the article: "Simulation of stresses in an innovative combination of composite with sheet".

  13. A study of ground-structure interaction in dynamic plate load testing

    Science.gov (United States)

    Guzina, Bojan B.; Nintcheu Fata, Sylvain

    2002-10-01

    A mathematical treatment is presented for the forced vertical vibration of a padded annular footing on a layered viscoelastic half-space. On assuming a depth-independent stress distribution for the interfacial buffer, the set of triple integral equations stemming from the problem is reduced to a Fredholm integral equation of the second kind. The solution method, which is tailored to capture the stress concentrations beneath footing edges, is highlighted. To cater to small-scale geophysical applications, the model is used to investigate the near-field effects of ground-loading system interaction in dynamic geotechnical and pavement testing. Numerical results indicate that the uniform-pressure assumption for the contact load between the composite disc and the ground which is customary in dynamic plate load testing may lead to significant errors in the diagnosis of subsurface soil and pavement conditions. Beyond its direct application to non-intrusive site characterization, the proposed solution can be used in the seismic analysis of a variety of structures involving annular foundation geometries.

  14. Analysis and seismic tests of composite shear walls with CFST columns and steel plate deep beams

    Science.gov (United States)

    Dong, Hongying; Cao, Wanlin; Wu, Haipeng; Zhang, Jianwei; Xu, Fangfang

    2013-12-01

    A composite shear wall concept based on concrete filled steel tube (CFST) columns and steel plate (SP) deep beams is proposed and examined in this study. The new wall is composed of three different energy dissipation elements: CFST columns; SP deep beams; and reinforced concrete (RC) strips. The RC strips are intended to allow the core structural elements — the CFST columns and SP deep beams — to work as a single structure to consume energy. Six specimens of different configurations were tested under cyclic loading. The resulting data are analyzed herein. In addition, numerical simulations of the stress and damage processes for each specimen were carried out, and simulations were completed for a range of location and span-height ratio variations for the SP beams. The simulations show good agreement with the test results. The core structure exhibits a ductile yielding mechanism characteristic of strong column-weak beam structures, hysteretic curves are plump and the composite shear wall exhibits several seismic defense lines. The deformation of the shear wall specimens with encased CFST column and SP deep beam design appears to be closer to that of entire shear walls. Establishing optimal design parameters for the configuration of SP deep beams is pivotal to the best seismic behavior of the wall. The new composite shear wall is therefore suitable for use in the seismic design of building structures.

  15. Improved thermal-vacuum compatible flat plate radiometric source for system-level testing of remote optical sensors

    Science.gov (United States)

    Schwarz, Mark A.; Kent, Craig J.; Bousquet, Robert; Brown, Steven W.

    2016-09-01

    In this work, we describe an improved thermal-vacuum compatible flat plate radiometric source which has been developed and utilized for the characterization and calibration of remote optical sensors. This source is unique in that it can be used in situ, in both ambient and thermal-vacuum environments, allowing it to follow the sensor throughout its testing cycle. The performance of the original flat plate radiometric source was presented at the 2009 SPIE1. Following the original efforts, design upgrades were incorporated into the source to improve both radiometric throughput and uniformity. The pre-thermal-vacuum (pre-TVAC) testing results of a spacecraft-level optical sensor with the improved flat plate illumination source, both in ambient and vacuum environments, are presented. We also briefly discuss potential FPI configuration changes in order to improve its radiometric performance. Keywords: Calibration, radiometry, remote sensing, source.

  16. Synthesis for Lunar Simulants: Glass, Agglutinate, Plagioclase, Breccia

    Science.gov (United States)

    Weinstein, Michael; Wilson, Stephen A.; Rickman, Douglas L.; Stoeser, Douglas

    2012-01-01

    The video describes a process for making glass for lunar regolith simulants that was developed from a patented glass-producing technology. Glass composition can be matched to simulant design and specification. Production of glass, pseudo agglutinates, plagioclase, and breccias is demonstrated. The system is capable of producing hundreds of kilograms of high quality glass and simulants per day.

  17. Multivalent dendritic molecules as broad spectrum bacteria agglutination agents.

    Science.gov (United States)

    Xiao, Shuzhang; Abu-Esba, Lica; Turkyilmaz, Serhan; White, Alexander G; Smith, Bradley D

    2013-01-01

    This study reports the first set of synthetic molecules that act as broad spectrum agglutination agents and thus are complementary to the specific targeting of antibodies. The molecules have dendritic architecture and contain multiple copies of zinc(II)-dipicolylamine (ZnDPA) units that have selective affinity for the bacterial cell envelope. A series of molecular structures were evaluated, with the number of appended ZnDPA units ranging from four to thirty-two. Agglutination assays showed that the multivalent probes rapidly cross-linked ten different strains of bacteria, regardless of Gram-type and cell morphology. Fluorescence microscopy studies using probes with four ZnDPA units indicated a high selectivity for bacteria agglutination in the presence of mammalian cells and no measurable effect on the health of the cells. The high bacterial selectivity was confirmed by conducting in vivo optical imaging studies of a mouse leg infection model. The results suggest that multivalent ZnDPA molecular probes with dendritic structures have great promise as selective, broad spectrum bacterial agglutination agents for infection imaging and theranostic applications.

  18. Synthesis for Lunar Simulants: Glass, Agglutinate, Plagioclase, Breccia

    Science.gov (United States)

    Weinstein, Michael; Wilson, Stephen A.; Rickman, Douglas L.; Stoeser, Douglas

    2012-01-01

    The video describes a process for making glass for lunar regolith simulants that was developed from a patented glass-producing technology. Glass composition can be matched to simulant design and specification. Production of glass, pseudo agglutinates, plagioclase, and breccias is demonstrated. The system is capable of producing hundreds of kilograms of high quality glass and simulants per day.

  19. Preliminary evaluation of a gel tube agglutination major cross-match method in dogs.

    Science.gov (United States)

    Villarnovo, Dania; Burton, Shelley A; Horney, Barbara S; MacKenzie, Allan L; Vanderstichel, Raphaël

    2016-09-01

    A major cross-match gel tube test is available for use in dogs yet has not been clinically evaluated. This study compared cross-match results obtained using the gel tube and the standard tube methods for canine samples. Study 1 included 107 canine sample donor-recipient pairings cross-match tested with the RapidVet-H method gel tube test and compared results with the standard tube method. Additionally, 120 pairings using pooled sera containing anti-canine erythrocyte antibody at various concentrations were tested with leftover blood from a hospital population to assess sensitivity and specificity of the gel tube method in comparison with the standard method. The gel tube method had a good relative specificity of 96.1% in detecting lack of agglutination (compatibility) compared to the standard tube method. Agreement between the 2 methods was moderate. Nine of 107 pairings showed agglutination/incompatibility on either test, too few to allow reliable calculation of relative sensitivity. Fifty percent of the gel tube method results were difficult to interpret due to sample spreading in the reaction and/or negative control tubes. The RapidVet-H method agreed with the standard cross-match method on compatible samples, but detected incompatibility in some sample pairs that were compatible with the standard method. Evaluation using larger numbers of incompatible pairings is needed to assess diagnostic utility. The gel tube method results were difficult to categorize due to sample spreading. Weak agglutination reactions or other factors such as centrifuge model may be responsible. © 2016 American Society for Veterinary Clinical Pathology.

  20. HV Test of the CTS Edgeless Silicon Detector in Vacuum and Close to a Grounded Plate

    CERN Document Server

    Eremin, Vladimir; Ruggiero, Gennaro

    2007-01-01

    The TOTEM Roman Pot Silicon sensors will be operated in vacuum to minimise the mechanical stress of the thin metal window which separates the detector package from the ultra high vacuum of the beam. To approach the beam axis as close as possible the detectors will be mounted with their edge at a distance of the order 100 - 200 um from the thin metal window. As the detectors will be run in overdepletion mode to allow the full charge collection within the shaping time of the readout electronics, there will be a potential drop of more than 100 V across their edge. Moreover this potential drop might need to be further increased with the accumulated radiation dose. The main goals of the tests described in this note are: - Characterisation of the voltage-current characteristics when the detector edge is in the direct vicinity of a grounded metal plate which simulates the above mentioned vacuum window; - Demonstration of the detector operation in vacuum at different pressures.

  1. Testing Absolute Plate Reference Frames and the Implications for the Generation of Geodynamic Mantle Heterogeneity Structure

    Science.gov (United States)

    Shephard, G. E.; Bunge, H.; Schuberth, B. S.; Müller, D.; Talsma, A.; Moder, C.

    2010-12-01

    Several absolute reference frames for Cretaceous-Tertiary plate tectonic reconstructions have been proposed over the last decade. They include reference frames based on hotspot tracks displaying age progression, and assuming either fixed or moving hotspots, as well as palaeomagnetically-based reference frames, a subduction reference frame and hybrid reference frames. All these alternative reference frames imply a particular history of the location of subduction zones through time, the associated subduction history, and the evolution of mantle heterogeneity via the mixing of subducted slab material with the mantle. Therefore it is possible to evaluate the observed distribution of subducted slab material in the mantle versus that predicted by a forward geodynamic model in which the plate kinematic history given by a particular absolute plate is coupled with a mantle convection model. We present a comparison of five alternative absolute plate motion models in terms of their consequences for global deep mantle structure by utilizing the 3-D spherical finite element mantle convection code TERRA, coupled with the global plate tectonic reconstruction software GPlates. We impose global palaeo-plate boundaries and plate velocities back to 140 Ma as surface boundary conditions for each absolute rotation model and forward model the associated subduction history. The correlation of seismic tomography with the predicted present-day mantle structure from each of plate models is then assessed using well-imaged slabs. We will present and discuss a comparison of geodynamically predicted mantle heterogeneity and seismic tomography to infer the robustness of each absolute reference frame through time, thus providing additional constraints for the integration of plate tectonics and mantle dynamics.

  2. Cerebrospinal Fluid Treponema pallidum Particle Agglutination Assay for Neurosyphilis Diagnosis.

    Science.gov (United States)

    Marra, Christina M; Maxwell, Clare L; Dunaway, Shelia B; Sahi, Sharon K; Tantalo, Lauren C

    2017-06-01

    Limited data suggest that the cerebrospinal fluid Treponema pallidum particle agglutination assay (CSF-TPPA) is sensitive and a CSF Treponema pallidum hemagglutination assay (CSF-TPHA) titer of ≥1:640 is specific for neurosyphilis diagnosis. CSF-TPPA reactivity and titer were determined for a convenience sample of 191 CSF samples from individuals enrolled in a study of CSF abnormalities in syphilis (training data set). The sensitivity of a reactive test and the specificity for reactivity at serial higher CSF dilutions were determined. Subsequently, CSF-TPPA reactivity at a 1:640 dilution was determined for all available samples from study participants enrolled after the last training sample was collected (validation data set, n = 380). Neurosyphilis was defined as (i) a reactive CSF Venereal Disease Research Laboratory test (CSF-VDRL), (ii) detection of T. pallidum in CSF by reverse transcriptase PCR, or (iii) new vision loss or hearing loss. In the training data set, the diagnostic sensitivities of a reactive CSF fluorescent treponemal antibody absorption test (CSF-FTA-ABS) and a reactive CSF-TPPA did not differ significantly (67 to 98% versus 76 to 95%). The specificity of a CSF-TPPA titer of ≥1:640 was significantly higher than that of lower dilutions and was not significantly different from that of CSF-VDRL. In the validation data set, the diagnostic specificity of a CSF-TPPA titer of ≥1:640 was high and did not differ significantly from that of CSF-VDRL (93 to 94% versus 90 to 91%). Ten CSF samples with a nonreactive CSF-VDRL had a CSF-TPPA titer of ≥1:640. If a CSF-TPPA titer of ≥1:640 was used in addition to a reactive CSF-VDRL, the number of neurosyphilis diagnoses would have increased from 47 to 57 (21.3%). A CSF-TPPA titer cutoff of ≥1:640 may be useful in identifying patients with neurosyphilis when CSF-VDRL is nonreactive. Copyright © 2017 American Society for Microbiology.

  3. Comparison of agglutination test, microscopy and nPCR for ...

    African Journals Online (AJOL)

    0.3 µl external forward Primer (50 µM), 0.3 µl external reverse Primer. (50 µM each), 0.2 µl ... The cycling condition for PCRl was 5 minutes of Taq ... All efforts were made to minimize ani- mal suffering during the course of the study. Results.

  4. Development of a Titanium Plate for Mandibular Angle Fractures with a Bone Defect in the Lower Border: Finite Element Analysis and Mechanical Test

    Directory of Open Access Journals (Sweden)

    Douglas Rangel Goulart

    2015-09-01

    Full Text Available Objectives: The aim of the present study was to develop a plate to treat mandibular angle fractures using the finite element method and mechanical testing. Material and Methods: A three-dimensional model of a fractured mandible was generated using Rhinoceros 4.0 software. The models were exported to ANSYS®, in which a static application of displacement (3 mm was performed in the first molar region. Three groups were assessed according to the method of internal fixation (2 mm system: two non-locking plates; two locking plates and a new design locking plate. The computational model was transferred to an in vitro experiment with polyurethane mandibles. Each group contained five samples and was subjected to a linear loading test in a universal testing machine. Results: A balanced distribution of stress was associated with the new plate design. This plate modified the mechanical behavior of the fractured region, with less displacement between the fractured segments. In the mechanical test, the group with two locking plates exhibited greater resistance to the 3 mm displacement, with a statistically significant difference when compared with the new plate group (ANOVA, P = 0.016. Conclusions: The new plate exhibited a more balanced distribution of stress. However, the group with two locking plates exhibited greater mechanical resistance.

  5. The Study of Analgesic Effects of Leonurus cardiaca L. in Mice by Formalin, Tail Flick and Hot Plate Tests.

    Science.gov (United States)

    Rezaee-Asl, Masoume; Sabour, Mandana; Nikoui, Vahid; Ostadhadi, Sattar; Bakhtiarian, Azam

    2014-01-01

    Leonurus cardiaca, commonly known as motherwort, is a member of the Lamiaceae family. It has a number of interesting biological activities, for example, sedative and hypotensive, antioxidant, anti-inflammatory, and antimicrobial activities. The aim of the present study was to investigate the effect of alcoholic extract of aerial part of Leonurus cardiaca on nociceptive response using formalin, tail flick, and hot plate tests in mice. The acute treatment of mice with an ethanolic extract at doses of 500 and 250 mg/kg by intraperitoneal administration produced a significant antinociceptive in the first and second phases of formalin test, respectively. The hot plate and tail flick tests showed an increase in the antinociceptive effect at dose 500 mg/kg. These results suggest that Leonurus cardiaca possesses central and peripheral antinociceptive actions.

  6. Standard Test Method for Water Penetration of Flat Plate Solar Collectors by Uniform Static Air Pressure Difference

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    1986-01-01

    1.1 This test method covers the determination of the resistance of flat plate solar collectors to water penetration when water is applied to their outer surfaces with a static air pressure at the outer surface higher than the pressure at the interior of the collector. 1.2 This test method is applicable to any flat plate solar collector. 1.3 The proper use of this test method requires a knowledge of the principles of pressure and deflection measurement. 1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary information is contained in Section 6.

  7. DEM analyses of the whole failure process of shallow foundation in plate load test on dense sand

    Science.gov (United States)

    Li, L.; Jiang, M. J.; Li, T.; Chen, S. L.

    2015-09-01

    Shallow foundations are widely used in civil engineering practice, but the instability mechanism is still unclear yet. Previously, the Finite Element Method (FEM) was commonly used to analyze the failure process of shallow foundations, but it meets difficulty in properly simulating the whole failure process of shallow foundation on the strain-softening material. Hence, the Discrete Element Method (DEM) is employed in this paper to study the instability mechanism of the shallow foundation via numerical plate load test with focus on the microscopic features evolution during vertical loading. In the simulation, an amplified gravity was applied to a dense granular ground to reproduce a gravity stress state at a large scale. Then, a plate was put on the granular ground to simulate the plate load test. Deformation pattern, particle velocity and distribution of void ratio in the ground were examined to illustrate the microscopic features in the whole failure process of the granular ground. The results show that: 1) There are a marked peak value and a settlement softening branch in the stress-settlement relationship. 2) The grids close to the edge of the plate are peculiarly extended and twisted. 3) Four particle motion patterns were observed in the velocity fields and the percentage of each motion pattern changes during loading. 4) The void ratio field varies during loading, and the distinguishing interface tends to be similar to Terzaghi's shear failure surface.

  8. Three-contrast, metal test pattern (Snellen E-plate) in evaluation of imaging techniques in clinical chest radiography

    Energy Technology Data Exchange (ETDEWEB)

    Manninen, H.

    A metal test pattern based on a minified Snellen-type E-plate was evaluated in testing six imaging techniques for chest radiography. The device consisted of three contrast zones and it was attached to the patient's back over the right lung during chest radiography. The values for visual detectability of the E-figures were compared with the film scores obtained by grading the radiographs with respect to visibility of diagnostically important anatomic details. The test plate proved to be a simple, quick and reproducible tool for testing several imaging techniques at the same time. The E-plate results were similar to those of the visual grading analysis in most respects. A few discrepancies existed, which were probably due to the inability of the test pattern to fully consider the effect of motion unsharpness and varying scattering conditions within the human thorax, as well as to great differences in roentgen ray absorption properties between Au and tissues. (orig.).

  9. Mononucleosis spot test

    Science.gov (United States)

    Monospot test; Heterophile antibody test; Heterophile agglutination test; Paul-Bunnell test; Forssman antibody test ... The mononucleosis spot test is done when symptoms of mononucleosis are ... Fatigue Fever Large spleen (possibly) Sore throat Tender ...

  10. Evaluation of plate type fuel elements by eddy current test method; Avaliacao de combustiveis nucleares tipo placa pelo metodo de correntes parasitas

    Energy Technology Data Exchange (ETDEWEB)

    Frade, Rangel Teixeira

    2015-07-01

    Plate type fuel elements are used in MTR research nuclear reactors. The fuel plates are manufactured by assembling a briquette containing the fissile material inserted in a frame, with metal plates in both sides of the set, to act as a cladding. This set is rolled under controlled conditions in order to obtain the fuel plate. In Brazil, this type of fuel is manufactured by IPEN and used in the IEA-R1 reactor. After fabrication of three batches of fuel plates, 24 plates, one of them is taken, in order to verify the thickness of the cladding. For this purpose, the plate is sectioned and the thickness measurements are carried out by using optical microscopy. This procedure implies in damage of the plate, with the consequent cost. Besides, the process of sample preparation for optical microscopy analysis is time consuming, it is necessary an infrastructure for handling radioactive materials and there is a generation of radioactive residues during the process. The objective of this study was verify the applicability of eddy current test method for nondestructive measurement of cladding thickness in plate type nuclear fuels, enabling the inspection of all manufactured fuel plates. For this purpose, reference standards, representative of the cladding of the fuel plates, were manufactured using thermomechanical processing conditions similar to those used for plates manufacturing. Due to no availability of fuel plates for performing the experiments, the presence of the plate’s core was simulated using materials with different electrical conductivities, fixed to the thickness reference standards. Probes of eddy current testing were designed and manufactured. They showed high sensitivity to thickness variations, being able to separate small thickness changes. The sensitivity was higher in tests performed on the reference standards and samples without the presence of the materials simulating the core. For examination of the cladding with influence of materials simulating the

  11. Detection of plating defects using ultrasonic and eddy current test methods; Nachweis von Plattierungsfehlern mit Ultraschall- und Wirbelstromverfahren

    Energy Technology Data Exchange (ETDEWEB)

    Waidele, H.; Knoch, P. [MPA, Univ. Stuttgart (Germany); Gersinska, R. [BfS Salzgitter (Germany); Brenner, W.; Seidenkranz, T. [TUeV Sued (Germany); Csapo, G.; Just, T. [TUeV Nord (Germany); Weiss, R. [IzfP Saarbruecken (Germany); Rathgeb, W. [IntelligeNDT, Erlangen (Germany)

    2004-07-01

    The validity of ultrasonic and eddy current testing of austenitic platings of reactor pressure vessels was investigated in the context of a project funded by the Federal Radiation Protection Office (Bundesamt fuer Strahlenschutz). In the first phase, test specimens with natural defects were fabricated at MPA Stuttgart; in the second phase, plating defects were introduced in a MPA large-size vessel. Measurements showed that most plating defects will be detected by standard ultrasonic testing. The highest accuracy was achieved by a combination of ultrasonic and eddy current testing. (orig.) [German] Im Rahmen eines vom Bundesamt fuer Strahlenschutz gefoerderten Vorhabens wurde die Aussagefaehigkeit von Ultraschall- und Wirbelstrompruefungen an austenitischen Plattierungen von Reaktordruckbehaeltern untersucht. In der ersten Phase des Vorhabens wurden an der MPA Stuttgart Testkoerper mit natuerlichen Fehlern im Plattierungsbereich gefertigt, in einer zweiten Phase wurden Plattierungsfehler in den MPA-Grossbehaelter eingebracht. Die Messungen haben gezeigt, dass die meisten Plattierungsfehler mit Ultraschall-Standardprueftechniken nachweisbar sind, wobei die groesste Aussagekraft bei einer Kombination von Ultraschall- und Wirbelstrompruefung gegeben ist. (orig.)

  12. Displacement analysis of a bend plate test with mechanical loading and laser heating

    Energy Technology Data Exchange (ETDEWEB)

    Lam, P.S.

    1997-09-01

    The surface displacment of a steel plate caused by a permanent deformation as a result of local yielding was modeled by a finite element analysis. The local yielding occurs when a small area of the plate is heated by a laser beam. The calculated displacments are in good agreement with the preliminary experimental data obtained using a bend specimen with laser heating at the University of Alabama at Huntsville. It has been shown computuationally and optically that the relative displacments are less than 1mm near the laser heated area of the specimen. The results demonstrate that the experimental approach is a feasible technique for determining the residual stress under multiaxial stress field.

  13. Development of eddy current testing probe for thick-walled metal plate and quantitative evaluation of cracks

    CERN Document Server

    Sato, K; Uchimoto, T; Takagi, T

    2003-01-01

    This paper demonstrates the crack detection of thick-walled non-magnetic metal plates by eddy current testing, which is difficult because of Kelvin skin effect generally. The purpose of this research is the development of an new eddy current testing probe for cracks in thick-walled plates and crack shapes quantitative evaluation. The probe was designed, based on the numerical computation using 3D fast eddy current code. The advantages of this new probe are strong eddy current on the back of specimens and gentle decrement of eddy current in the thickness direction. Through experiments, we confirmed that this probe can detect the back artificial defect with 0.5 mm thickness on IN-CONEL 718 specimen with 7.0 mm thickness. Reconstruction of crack shapes was performed based on the experimental results with the inverse problem code developed by authors. The length and depth of reconstructed defects approximately agree with those of real crack. (author)

  14. Development and comparative evaluation of a plate enzyme-linked immunosorbent assay based on recombinant outer membrane antigens Omp28 and Omp31 for diagnosis of human brucellosis.

    Science.gov (United States)

    Tiwari, Sapana; Kumar, Ashu; Thavaselvam, Duraipandian; Mangalgi, Smita; Rathod, Vedika; Prakash, Archana; Barua, Anita; Arora, Sonia; Sathyaseelan, Kannusamy

    2013-08-01

    Brucellosis is an important zoonotic infectious disease of humans and livestock with worldwide distribution and is caused by bacteria of the genus Brucella. The diagnosis of brucellosis always requires laboratory confirmation by either isolation of pathogens or detection of specific antibodies. The conventional serological tests available for the diagnosis of brucellosis are less specific and show cross-reactivity with other closely related organisms. These tests also necessitate the handling of Brucella species for antigen preparation. Therefore, there is a need to develop reliable, rapid, and user-friendly systems for disease diagnosis and alternatives to vaccine approaches. Keeping in mind the importance of brucellosis as an emerging infection and the prevalence in India, we carried out the present study to compare the recombinant antigens with the native antigens (cell envelope and sonicated antigen) of Brucella for diagnosis of human brucellosis by an indirect plate enzyme-linked immunosorbent assay (ELISA). Recombinant outer membrane protein 28 (rOmp28) and rOmp31 antigens were cloned, expressed, and purified in the bacterial expression system, and the purified proteins were used as antigens. Indirect plate ELISAs were then performed and standardized for comparison of the reactivities of recombinant and native antigens against the 433 clinical samples submitted for brucellosis testing, 15 culture-positive samples, and 20 healthy donor samples. The samples were separated into four groups based on their positivity to rose bengal plate agglutination tests (RBPTs), standard tube agglutination tests (STATs), and 2-mercaptoethanol (2ME) tests. The sensitivities and specificities of all the antigens were calculated, and the rOmp28 antigen was found to be more suitable for the clinical diagnosis of brucellosis than the rOmp31 antigen and native antigens. The rOmp28-based ELISA showed a very high degree of agreement with the conventional agglutination tests and

  15. Standarized performance tests of collectors of solar thermal energy: A steel flat-plate collector with two transparent covers and a proprietary coating

    Science.gov (United States)

    1976-01-01

    Basic test results of a flat-plate solar collector whose performance was determined in the NASA-Lewis solar simulator are given. The collector was tested over ranges of inlet temperature and flux level.

  16. Synthesis of Rh/Macro-Porous Alumina Over Micro-Channel Plate and Its Catalytic Activity Tests for Diesel Reforming.

    Science.gov (United States)

    Seong, Yeon Baek; Kim, Yong Sul; Park, No-Kuk; Lee, Tae Jin

    2015-11-01

    Macro-porous Al2O3 as the catalytic support material was synthesized using colloidal polystyrene spheres over a micro-channel plate. The colloidal polystyrene spheres were used as a template for the production of an ordered macro porous material using an alumina nitrate solution as the precursor for Al2O3. The close-packed colloidal crystal array template method was applied to the formulation of ordered macro-porous Al2O3 used as a catalytic support material over a micro-channel plate. The solvent in the mixture solution, which also contained the colloidal polystyrene solution, aluminum nitrate solution and the precursor of the catalytic active materials (Rh), was evaporated in a vacuum oven at 50 degrees C. The ordered polystyrene spheres and aluminum salt of the solid state were deposited over a micro channel plate, and macro-porous Al2O3 was formed after calcination at 600 degrees C to remove the polystyrene spheres. The catalytic activity of the Rh/macro-porous alumina supported over the micro-channel plate was tested for diesel reforming.

  17. Requirements and testing methods for surfaces of metallic bipolar plates for low-temperature PEM fuel cells

    Science.gov (United States)

    Jendras, P.; Lötsch, K.; von Unwerth, T.

    2017-03-01

    To reduce emissions and to substitute combustion engines automotive manufacturers, legislature and first users aspire hydrogen fuel cell vehicles. Up to now the focus of research was set on ensuring functionality and increasing durability of fuel cell components. Therefore, expensive materials were used. Contemporary research and development try to substitute these substances by more cost-effective material combinations. The bipolar plate is a key component with the greatest influence on volume and mass of a fuel cell stack and they have to meet complex requirements. They support bending sensitive components of stack, spread reactants over active cell area and form the electrical contact to another cell. Furthermore, bipolar plates dissipate heat of reaction and separate one cell gastight from the other. Consequently, they need a low interfacial contact resistance (ICR) to the gas diffusion layer, high flexural strength, good thermal conductivity and a high durability. To reduce costs stainless steel is a favoured material for bipolar plates in automotive applications. Steel is characterized by good electrical and thermal conductivity but the acid environment requires a high chemical durability against corrosion as well. On the one hand formation of a passivating oxide layer increasing ICR should be inhibited. On the other hand pitting corrosion leading to increased permeation rate may not occur. Therefore, a suitable substrate lamination combination is wanted. In this study material testing methods for bipolar plates are considered.

  18. Seroprevalencia de leptospirosis canina en perros atendidos en clínicas veterinarias, mediante aglutinación microscópica y comparación con las técnicas de aislamiento e inmunofluorescencia indirecta Frequency of canine leptospirosis in dogs attending veterinary practices determined through microscopic agglutination test and comparison with isolation and immunofluorescence techniques

    Directory of Open Access Journals (Sweden)

    R F Silva

    2007-01-01

    estos individuos reaccionaron al test de aglutinación microscópica - MAT (14% y a la inmunofluorescencia indirecta - IFI (10%. El suero hiperinmune obtenido para la realización de esta última técnica no sería serovar específico. El test de aglutinación microscópica presentó mayor sensibilidad y especificidad que las técnicas de aislamiento e inmunofluorescencia indirectaThe aim of this study was to determine the frequency of leptospirosis in 400 dogs from rural and urban areas that attended veterinary practices in Valdivia (Chile, using the Microscopic Agglutination Test (MAT. Each serum sample was tested against eight serovars of leptospira (hardjo, pomona, canicola, ballum, icterohaemorrhagiae, tarassovi, grippotyphosa, autumnalis, 14.8% of the dogs showed a positive leptospirosis title with the majority of them reacting to the serovars canicola, icterohaemorrhagiae and ballum. Most of these sera had titles between 1/400 and > 1/1600. The results presented in this study do not show a significant difference in relation to gender, origin (urban/rural, breed or vaccination status. A relationship was found between the state of dogs living in the wild and the probability of contracting the disease and vaccinated dogs in relation to the infection with the serovar ballum, being much more likely for vaccinated dogs to contract an infection with this serovar than it is for unvaccinated dogs. Additionally, the study compared the MAT, isolation and indirect immunofluorescence (IFl techniques using renal tissue of 50 urban dogs. For the IFl study, hyperimmuneserum was obtained from rats inoculated with the serovars canicola and icterohaemorrhagiae. Isolation from renal tissue was unsuccessful, although 14% showed a positive response to MAT and 10% were positively identified with IFL The hyperimmuneserum used in the IFl technique was not serovar specific. The Microscopic Agglutination Test showed a higher sensibility and specificity compared to the isolation and

  19. Measurement of RBC agglutination with microscopic cell image analysis in a microchannel chip.

    Science.gov (United States)

    Cho, Chi Hyun; Kim, Ju Yeon; Nyeck, Agnes E; Lim, Chae Seung; Hur, Dae Sung; Chung, Chanil; Chang, Jun Keun; An, Seong Soo A; Shin, Sehyun

    2014-01-01

    Since Landsteiner's discovery of ABO blood groups, RBC agglutination has been one of the most important immunohematologic techniques for ABO and RhD blood groupings. The conventional RBC agglutination grading system for RhD blood typings relies on macroscopic reading, followed by the assignment of a grade ranging from (-) to (4+) to the degree of red blood cells clumping. However, with the new scoring method introduced in this report, microscopically captured cell images of agglutinated RBCs, placed in a microchannel chip, are used for analysis. Indeed, the cell images' pixel number first allows the differentiation of agglutinated and non-agglutinated red blood cells. Finally, the ratio of agglutinated RBCs per total RBC counts (CRAT) from 90 captured images is then calculated. During the trial, it was observed that the agglutinated group's CRAT was significantly higher (3.77-0.003) than that of the normal control (0). Based on these facts, it was established that the microchannel method was more suitable for the discrimination between agglutinated RBCs and non-agglutinated RhD negative, and thus more reliable for the grading of RBCs agglutination than the conventional method.

  20. Test of a four-gap resistive plate chamber with cosmic muons and high-rate gamma rays

    Energy Technology Data Exchange (ETDEWEB)

    Park, S.K.; Shin, S.S.; Hong, B.; Jhang, G.; Jeong, M.S.; Jo, M.; Cho, S.W.; Joo, E.; Kim, C.; Kim, H.C. [Department of Physics and Korea Detector Laboratory, Korea University, Seoul 136-701 (Korea, Republic of); Lee, K.S., E-mail: kslee0421@korea.ac.kr [Department of Physics and Korea Detector Laboratory, Korea University, Seoul 136-701 (Korea, Republic of); Lee, K.S.; Lee, S.; Lee, S.K.; Lim, J.K.; Moon, D.H.; Shim, H.H.; Sim, K.S. [Department of Physics and Korea Detector Laboratory, Korea University, Seoul 136-701 (Korea, Republic of)

    2012-07-11

    We report a new development of a phenolic four-gap resistive plate chamber (RPC) for use as a high-rate particle trigger in high-energy physics experiments. In the current study, a prototype RPC equipped with four 1-mm-thick gaps made from high-pressure-laminated (HPL) resistive plates has been designed and built. The current detector R and D aims to reduce the probability of radiation-induced degradation of the core detector components of the RPCs, the gas gaps, as well as to improve the detection rate capability. The detector characteristics of the four-gap RPC were obtained though a series of tests with cosmic muons and gamma rays and were compared with those of the typical 2-mm thick double-gap RPCs currently used in the CMS experiment at the LHC and in the PHENIX experiment at the RHIC.

  1. Amyloidogenic amyloid-β-peptide variants induce microbial agglutination and exert antimicrobial activity

    Science.gov (United States)

    Spitzer, Philipp; Condic, Mateja; Herrmann, Martin; Oberstein, Timo Jan; Scharin-Mehlmann, Marina; Gilbert, Daniel F.; Friedrich, Oliver; Grömer, Teja; Kornhuber, Johannes; Lang, Roland; Maler, Juan Manuel

    2016-01-01

    Amyloid-β (Aβ) peptides are the main components of the plaques found in the brains of patients with Alzheimer’s disease. However, Aβ peptides are also detectable in secretory compartments and peripheral blood contains a complex mixture of more than 40 different modified and/or N- and C-terminally truncated Aβ peptides. Recently, anti-infective properties of Aβ peptides have been reported. Here, we investigated the interaction of Aβ peptides of different lengths with various bacterial strains and the yeast Candida albicans. The amyloidogenic peptides Aβ1-42, Aβ2-42, and Aβ3p-42 but not the non-amyloidogenic peptides Aβ1-40 and Aβ2-40 bound to microbial surfaces. As observed by immunocytochemistry, scanning electron microscopy and Gram staining, treatment of several bacterial strains and Candida albicans with Aβ peptide variants ending at position 42 (Aβx-42) caused the formation of large agglutinates. These aggregates were not detected after incubation with Aβx-40. Furthermore, Aβx-42 exerted an antimicrobial activity on all tested pathogens, killing up to 80% of microorganisms within 6 h. Aβ1-40 only had a moderate antimicrobial activity against C. albicans. Agglutination of Aβ1-42 was accelerated in the presence of microorganisms. These data demonstrate that the amyloidogenic Aβx-42 variants have antimicrobial activity and may therefore act as antimicrobial peptides in the immune system. PMID:27624303

  2. [Evaluation of the Fundamental Performance of 4 Latex Agglutination Reagents to Measure Anti-TP Antibody Concentration and Detailed Investigation of Decision-Mismatched Samples].

    Science.gov (United States)

    Ito, Atsushi; Niizeki, Noriyasu; Kurose, Hitomi; Yonezawa, Takatoshi; Sasaki, Rie; Tachibana, Mineji; Tomoda, Yutaka; Kino, Shuichi; Fujii, Satoshi

    2015-01-01

    Serological diagnosis of syphilis can be made by using the serological test for syphilis (STS) method for detecting a lipid antibody and Treponema pallidum (TP) method for detecting the anti-TP-specific antibody. In STS and TP methods, the basis using latex agglutination reaction has been used in many facilities. However, in latex agglutination, false-positive results due to non-specific reaction have sometimes been obtained in reactions of a routine laboratory test reagent detecting the anti-TP antibody used in our medical laboratory. We evaluated the fundamental performance of 4 reagents to measure anti-TP antibody concentration using latex agglutination: Reagents A, B, C and D produced by SEKISUI MEDICAL, FUJI REBIO, DENKA SEIKEN and SHINO TEST, respectively. We examined the correlations between Reagent A (routine laboratory test reagent) and Reagents B, C, and D in sera from 68 patients, and we performed additional investigation by using a neutralization test, immunochromatography, Western blotting, FTA-ABS (IgG), and STS method by an automatic analyzer for 13 decision-mismatched samples. The fundamental performance of each reagent was as good as that previously reported. Eight of the 13 decision-mismatched samples were false positives due to non-specific reaction of Reagent A. In latex agglutination non-specific reaction is inevitable. However, this study strongly suggests that using a neutralization test and immunochromatography that can be performed quickly is sufficient to verify whether positive reactions are true or false.

  3. Prevalence of agglutinating antibodies to Toxoplasma gondii and Sarcocystis neurona in beavers (Castor canadensis) from Massachusetts

    Science.gov (United States)

    Jordan, C.N.; Kaur, T.; Koenen, K.; DeStefano, S.; Zajac, A.M.; Lindsay, D.S.

    2005-01-01

    The present study examined the seroprevalence of Toxoplasma gondii and Sarcocystls neurona in a population of beavers (Castor canadensis) from Massachusetts. Sixty-two blood samples were collected during the field seasons over 3 consecutive years from different animals. Blood was collected onto filter paper and shipped to the Department of Biomedical Sciences, Virginia Tech, Blacksburg, Virginia, for parasite testing. The samples were tested at dilutions of 1:25, 1:50, and 1:100 against each parasite antigen by modified agglutination tests to determine whether antibodies to either parasite were present in the blood. Six of 62 samples (10%) were positive for T. gondii, with 2 samples having titers of 1:25 and 4 having titers of 1:50. Four of 62 samples (6%) were positive for S. neurona, with 2 samples having titers of 1:25 and 2 having titers of 1:50. ?? American Society of Pathologists 2005.

  4. Agglutination of human O erythrocytes by influenza A(H1N1) viruses freshly isolated from patients.

    Science.gov (United States)

    Murakami, T; Haruki, K; Seto, Y; Kimura, T; Minoshiro, S; Shibe, K

    1991-04-01

    The hemagglutinin titers of 10 influenza A (H1N1) viruses were examined using the erythrocytes of several species. Human O erythrocytes showed the highest agglutination titer to the viruses, whereas chicken erythrocytes showed a low titer. These findings were noted for at least 10 passages by serial dilutions of the viruses in Madin-Darby canine kidney (MDCK) cells. All influenza A(H1N1) viruses, plaque-cloned directly from throat-washing specimens of patients, also agglutinated human O but not chicken erythrocytes. The results of a hemadsorption test indicated that chicken erythrocytes possess less affinity to MDCK cells infected with the A/Osaka City/2/88(H1N1) stain than to those infected with the A/Yamagata/120/86(H1N1) strain which is used as an inactivated influenza vaccine in Japan. However, there were no significant differences between the A/Osaka City/2/88 and the A/Yamagata/120/86 strains in the hemagglutination inhibition test. Since human O erythrocytes have high agglutination activity to influenza A(H1N1) and also to A(H3N2) and B viruses in MDCK cells, these erythrocytes may be useful for the serological diagnosis of influenza.

  5. 混合抗球蛋白反应试验与体外受精-胚胎移植治疗关系的研究%Study of the Relationship between the Mixed Agglutination Reaction Test and Outcome of in vitro Fertilization-Embryo Transfer

    Institute of Scientific and Technical Information of China (English)

    孙源; 刘见桥; 杜红姿; 周华

    2012-01-01

    目的:探讨混合抗球蛋白反应试验(MAR)与常规体外受精-胚胎移植(IVF-ET)治疗的关系.方法:回顾性分析562例第1次行IVF治疗不孕患者的临床资料,按照MAR检测结果将IVF周期分成4个区间组:<10%,10%~30%,30%~50%,>50%,分析各组间受精率、胚胎发育及临床妊娠情况.结果:540个IVF移植周期中共获得279例妊娠,周期妊娠率为51.7%.MAR>50%组的受精率显著低于其余各组(P<0.05),而各组间女方年龄、不孕年限、受精失败率、精子密度、精子活动率、正常形态精子百分率、优质胚胎率、胚胎种植率、临床妊娠率及流产率比较则均无统计学差异(P>0.05).结论:MAR检测结果在预测IVF受精结局中有一定的价值,但与IVF治疗的临床妊娠结局无关.%Objective: To assess the relationship between the mixed agglutination reaction (MAR) test and outcome of in vitro fertilization-embryo transfer (IVF-ET) outcome. Methods: The clinical data of 562 infertile couples who underwent IVF treatment was analyzed by a retrospective study. According to the MAR test, the patients were divided into four groups: 50%. The fertilization rate, embryonic development and clinical pregnancy outcomes among the four groups were compared by statistical analysis. Results: A total of 22 cycles of total fertilization failure and 279 clinical pregnancies were obtained and the overall pregnancy rate was 51.7% per cycle. The maternal age, duration of infertility, total fertilization failure rate, sperm density, progressive motility, normal morphology, good-quality embryo rate, implantation rate, clinical pregnancy and abortion rate were not significantly different among these groups (P>0.05). However, the fertilization rate of the group with MAR>50% was significantly lower than other groups (P<0.05). Conclusion: The MAR test is valuable to predict the fertilization rate at IVF, however, the results of MAR test are not related to clinical

  6. The Effects of Morphine Sulfate on Agglutination, Clot Formation and Hemolysis in Packed Red Blood Cells

    Science.gov (United States)

    2007-11-02

    THE EFFECTS OF MORPHINE SULFATE ON AGGLUTINATION , CLOT FORMATION AND HEMOLYSIS IN PACKED RED BLOOD CELLS 6. AUTHOR(S) CAPT ESTAVILLO BRIAN K 7...ANSI Std8 239.18 Designed using Perform Pro, WHS/DIOR, Oct 94 THE EFFECTS OF MORPHINE SULFATE ON AGGLUTINATION , CLOT FORMATION AND HEMOLYSIS IN PACKED...that the use of any copyrighted material in the thesis entitled: "THE EFFECTS OF MORPHINE SULFATE ON AGGLUTINATION , CLOT FORMATION AND HEMOLYSIS IN

  7. Test and Numerical Analysis for Penetration Residual Velocity of Bullet Considering Failure Strain Uncertainty of Composite Plates

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Myungseok; Lee, Minhyung [Sejong Univ., Sejong (Korea, Republic of)

    2016-03-15

    The ballistic performance data of composite materials is distributed due to material inhomogeneity. In this paper, the uncertainty in residual velocity is obtained experimentally, and a method of predicting it is established numerically for the high-speed impact of a bullet into laminated composites. First, the failure strain distribution was obtained by conducting a tensile test using 10 specimens. Next, a ballistic impact test was carried out for the impact of a fragment-simulating projectile (FSP) bullet with 4ply ([0/90]s) and 8ply ([0/90/0/90]s) glass fiber reinforced plastic (GFRP) plates. Eighteen shots were made at the same impact velocity and the residual velocities were obtained. Finally, simulations were conducted to predict the residual velocities by using the failure strain distributions that were obtained from the tensile test. For this simulation, two impact velocities were chosen at 411.7m/s (4ply) and 592.5m/s (8ply). The simulation results show that the predicted residual velocities are in close agreement with test results. Additionally, the modeling of a composite plate with layered solid elements requires less calculation time than modeling with solid elements.

  8. Experimental tests of irradiation-anneal-reirradiation effects on mechanical properties of RPV plate and weld materials

    Energy Technology Data Exchange (ETDEWEB)

    Hawthorne, J.R. [Materials Engineering Associates, Inc., Lanham, MD (United States)

    1996-01-01

    The Charpy-V (C{sub V}) notch ductility and tension test properties of three reactor pressure vessel (RPV) steel materials were determined for the 288{degree}C (550{degree}F) irradiated (I), 288{degree}C (550{degree}F) irradiated + 454{degree}C (850{degree}F)-168 h postirradiation annealed (IA), and 288{degree}C (550{degree}F) reirradiated (IAR) conditions. Total fluences of the I condition and the IAR condition were, respectively, 3.33 {times} 10{sup 19} n/cm{sup 2} and 4.18 {times} 10{sup 19} n/cm{sup 2}, E > 1 MeV. The irradiation portion of the IAR condition represents an incremental fluence increase of 1. 05 {times} 10{sup 19} n/cm{sup 2}, E > 1 MeV, over the I-condition fluence. The materials (specimens) were supplied by the Yankee Atomic Electric Company and represented high and low nickel content plates and a high nickel, high copper content weld deposit prototypical of the Yankee-Rowe reactor vessel. The promise of the IAR method for extending the fluence tolerance of radiation-sensitive steels and welds is clearly shown by the results. The annealing treatment produced full C{sub V} upper shelf recovery and full or nearly full recovery in the C{sub V} 41 J (30 ft-lb) transition temperature. The C{sub V} transition temperature increases produced by the reirradiation exposure were 22% to 43% of the increase produced by the first cycle irradiation exposure. A somewhat greater radiation embrittlement sensitivity and a somewhat greater reirradiation embrittlement sensitivity was exhibited by the low nickel content plate than the high nickel content plate. Its high phosphorus content is believed to be responsible. The IAR-condition properties of the surface vs. interior regions of the low nickel content plate are also compared.

  9. Development of three dimensional Eulerian numerical procedure toward plate-mantle simulation: accuracy test by the fluid rope coiling

    Science.gov (United States)

    Furuichi, M.; Kameyama, M.; Kageyama, A.

    2007-12-01

    Reproducing a realistic plate tectonics with mantle convection simulation is one of the greatest challenges in computational geophysics. We have developed a three dimensional Eulerian numerical procedure toward plate-mantle simulation, which includes a finite deformation of the plate in the mantle convection. Our method, combined with CIP-CSLR (Constrained Interpolation Profile method-Conservative Semi-Lagrangian advection scheme with Rational function) and ACuTE method, enables us to solve advection and force balance equations even with a large and sharp viscosity jump, which marks the interface between the plates and surrounding upper mantle materials. One of the typical phenomena represented by our method is a fluid rope coiling event, where a stream of viscous fluid is poured onto the bottom plane from a certain height. This coiling motion is due to delicate balances between bending, twisting and stretching motions of fluid rope. In the framework of the Eulerian scheme, the fluid rope and surrounding air are treated as a viscosity profile which differs by several orders of magnitude. Our method solves the complex force balances of the fluid rope and air, by a multigrid iteration technique of ACuTE algorithm. In addition, the CIP-CSLR advection scheme allows us to obtain a deforming shape of the fluid rope, as a low diffusive solution in the Eulerian frame of reference. In this presentation, we will show the simulation result of the fluid rope coiling as an accuracy test for our simulation scheme, by comparing with the simplified numerical solution for thin viscous jet.

  10. Leptospirosis serosurvey in bovines from Brazilian Pantanal using IGG ELISA with recombinant protein LipL32 and microscopic agglutination test Sorodiagnóstico de leptospirose em bovinos do Pantanal brasileiro utilizando ELISA IgG com proteína recombinante LipL32 e soroaglutinação microscópica

    Directory of Open Access Journals (Sweden)

    Renata Graça Pinto Tomich

    2007-12-01

    Full Text Available This investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region's climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISA with recombinant protein LipL32 in comparison with microscopic agglutination test (MAT and additionally contribute to the knowledge of the distribution of the one of most important worldwide zoonotic infection, assessing the seropositivity of bovine leptospirosis in beef cattle herds of Brazilian Pantanal, an important ecological preserved area, where cattle constitute not only the most important economic resource but also the major activity compatible of the conservation of natural resource of the region. Out of 282 samples of cattle serum analyzed, 143 (50.71% were positive in MAT. The serovar Hardjo (genotypic Hardjoprajitno and Hardjobovis, Wolffi and Ballum showed the largest frequency of reactive samples. In the IgG ELISA rLipL32, 161 samples (57.09% were positive. This result was higher than obtained by MAT (pEste estudo foi realizado no Pantanal brasileiro: região que apresenta importante biodiversidade. As condições de clima, hidrologia e geomorfologia dessa região, bem como a existência de grande variedade de espécies animais silvestres, favorecem a manutenção da Leptospira no meio ambiente. O objetivo desse estudo foi avaliar o ELISA IgG com proteína recombinante LipL32 em comparação com a soroaglutinação microscópica (SAM para o diagnóstico sorológico de Leptospira. Adicionalmente, contribuir para o conhecimento da distribuição da leptospirose bovina, uma das mais importantes zoonoses mundialmente distribuída. Foi avaliada a soropositividade para essa bactéria em rebanhos bovinos de corte da região do Pantanal, uma área onde o bovino constitui não apenas o recurso econômico mais importante

  11. Quantitative Determination of Fibrinogen of Patients with Coronary Heart Diseases through Piezoelectric Agglutination Sensor

    Directory of Open Access Journals (Sweden)

    Ming Chen

    2010-03-01

    Full Text Available Fibrinogen can transform fibrin through an agglutination reaction, finally forming fibrin polymer with grid structure. The density and viscosity of the reaction system changes drastically during the course of agglutination. In this research, we apply an independently-developed piezoelectric agglutination sensor to detect the fibrinogen agglutination reaction in patients with coronary heart diseases. The terminal judgment method of determining plasma agglutination reaction through piezoelectric agglutination sensor was established. In addition, the standard curve between plasma agglutination time and fibrinogen concentration was established to determinate fibrinogen content quantitatively. The results indicate the close correlation between the STAGO paramagnetic particle method and the method of piezoelectric agglutination sensor for the detection of Fibrinogen. The correlation coefficient was 0.91 (γ = 0.91. The determination can be completed within 10 minutes. The fibrinogen concentration in the coronary heart disease group was significantly higher than that of the healthy control group (P < 0.05. The results reveal that high fibrinogen concentration is closely correlated to the incurrence, development and prognosis of coronary heart diseases. Compared with other traditional methods, the method of piezoelectric agglutination sensor has some merits such as operation convenience, small size, low cost, quick detecting, good precision and the common reacting agents with paramagnetic particle method.

  12. Quantitative determination of fibrinogen of patients with coronary heart diseases through piezoelectric agglutination sensor.

    Science.gov (United States)

    Chen, Qinghai; Hua, Xing; Fu, Weiling; Liu, Dongbo; Chen, Ming; Cai, Guoru

    2010-01-01

    Fibrinogen can transform fibrin through an agglutination reaction, finally forming fibrin polymer with grid structure. The density and viscosity of the reaction system changes drastically during the course of agglutination. In this research, we apply an independently-developed piezoelectric agglutination sensor to detect the fibrinogen agglutination reaction in patients with coronary heart diseases. The terminal judgment method of determining plasma agglutination reaction through piezoelectric agglutination sensor was established. In addition, the standard curve between plasma agglutination time and fibrinogen concentration was established to determinate fibrinogen content quantitatively. The results indicate the close correlation between the STAGO paramagnetic particle method and the method of piezoelectric agglutination sensor for the detection of Fibrinogen. The correlation coefficient was 0.91 (γ = 0.91). The determination can be completed within 10 minutes. The fibrinogen concentration in the coronary heart disease group was significantly higher than that of the healthy control group (P high fibrinogen concentration is closely correlated to the incurrence, development and prognosis of coronary heart diseases. Compared with other traditional methods, the method of piezoelectric agglutination sensor has some merits such as operation convenience, small size, low cost, quick detecting, good precision and the common reacting agents with paramagnetic particle method.

  13. Experimental testing of various heat transfer structures in a flat plate thermal energy storage unit

    Science.gov (United States)

    Johnson, Maike; Fiß, Michael; Klemm, Torsten

    2016-05-01

    For solar process heat applications with steam as the working fluid and varying application parameters, a novel latent heat storage concept has been developed using an adaptation of a flat plate heat exchanger as the storage concept. Since the pressure level in these applications usually does not exceed 30 bar, an adaptation with storage material chambers arranged between heat transfer medium chambers is possible. Phase change materials are used as the storage medium, so that the isothermal evaporation of steam during discharging of the storage is paired with the isothermal solidification of the storage material. Heat transfer structures can be inserted into the chambers to adjust the power level for a given application. By combining the required number of flat plate heat exchanger compartments and inserting the appropriate heat transfer structure, the design can easily be adjusted for the required power level and capacity for a specific application. Within this work, the technical feasibility of this concept is proven. The dependence of the operating characteristics on the geometry of the heat exchanger is identified. A focus is on varying the power density by integrating conductive heat structures in the PCM.

  14. Synergistic interaction of gabapentin with tiagabine in the hot-plate test in mice: an isobolographic analysis.

    Science.gov (United States)

    Luszczki, Jarogniew J; Kołacz, Agnieszka; Wojda, Ewa; Czuczwar, Mirosław; Przesmycki, Krzysztof; Czuczwar, Stanisław J

    2009-01-01

    This study was aimed at determining the analgesic effect of gabapentin and tiagabine, two antiepileptic drugs that were administered alone and in combination at a fixed ratio of 1:1, in the acute thermal pain model (hot-plate test) in mice. Linear regression analysis was used to evaluate the dose-response relationships between logarithms of antiepileptic drug doses and their resultant maximum possible antinociceptive effects in the mouse hot-plate test. From linear equations, we calculated doses that increased the antinociceptive effect by 50% (ED(50) values) for gabapentin, tiagabine and their combination. The type of interaction between gabapentin and tiagabine was assessed using the isobolographic analysis. Results indicated that both antiepileptic drugs produced the definite antinociceptive effect, and the experimentally derived ED(50) values for gabapentin and tiagabine, when applied alone, were 504.4 mg/kg and 5.67 mg/kg, respectively. With isobolography, the experimentally derived ED(50 mix) value for the fixed ratio combination of 1:1 was 139.31 mg/kg and significantly differed from the theoretically calculated ED(50 add) value, which was 255.04 mg/kg (p < 0.05), indicating the synergistic interaction between gabapentin and tiagabine in the hot-plate test in mice. In conclusion, the combination of tiagabine with gabapentin at a fixed ratio of 1:1 exerted a synergistic interaction in the mouse model of nociceptive pain. If the results from this study could be extrapolated to clinical settings, the combination of tiagabine with gabapentin might be beneficial for pain relief in humans.

  15. Development of a slide agglutination assay for detection of blastomycosis.

    Science.gov (United States)

    Hatch, Wayne O; Scalarone, Gene M

    2013-11-01

    Blastomycosis, caused by the thermally dimorphic fungus Blastomyces dermatitides, which is endemic to eastern regions of the USA, is commonly misdiagnosed as a viral or bacterial infection and therefore treated improperly. Over the years, many immunodiagnostic assays to aid in the diagnosis of blastomycosis have been developed; however, a reliable assay for use in local clinics still remains elusive. Procedures for a slide agglutination assay for detection of antibody in serum from rabbits immunized with B. dermatitidis were evaluated with antigenic preparations from B. dermatitidis adsorbed to polystyrene microparticles. Yeast-phase lysates from five isolates of B. dermatitides: namely ER-593 (Eagle River, WI, USA), ER-598 (Eagle River, WI, USA), 48938 (India), B5896 (Mt. Iron, MN, USA), and T-58 (TN, USA) were evaluated for their sensitivity and specificity. Sensitivities of the lysates ranged from 29% to 83% whereas specificities ranged from 13% to 100%. Lysate ER-593 provided the most promising results with a sensitivity of 82% and specificity of 100%. This study provides suggests that a simple rapid slide agglutination assay for detecting blastomycosis may be used for screening patients with suspected B. dermatitidis infection.

  16. Performance testing of a long-strip two-end readout multi-gap resistive plate chamber

    Institute of Scientific and Technical Information of China (English)

    WANG Xiao-Bin; WANG Yi; LUO Ming; LI Yuan-Jing; CHENG Jian-Ping

    2009-01-01

    Multi-gap Resistive Plate Chamber (MRPC) is a new generation of gas detector with good tim-ing and spacial resolution, whose technique is widely applied in some recent high energy (nuclear) physics experiments. In this letter, we report a long-strip two-end readout MRPC and its test beam performance. The measurements show that the long-strip performs a transmission line characteristic and the impedance is independent of the length of strip. The MRPC module we developed is presented to gain a timing resolution of~80 ps and a spacial resolution of ~6.4 mm. The possible application of the MRPC is also discussed.

  17. Antinociceptive effects of voluntarily ingested buprenorphine in the hot-plate test in laboratory rats.

    Science.gov (United States)

    Hestehave, Sara; Munro, Gordon; Pedersen, Tina Brønnum; Abelson, Klas S P

    2016-09-27

    Researchers performing experiments on animals should always strive towards the refinement of experiments, minimization of stress and provision of better animal welfare. An adequate analgesic strategy is important to improve post-operative recovery and welfare in laboratory rats and mice. In addition, it is desirable to provide post-operative analgesia using methods that are minimally invasive and stressful. This study investigated the antinociceptive effects of orally administered buprenorphine ingested in Nutella® in comparison with subcutaneous buprenorphine administration. By exposing the animal to a thermal stimulus using a hot plate, significant antinociceptive effects of voluntarily ingested buprenorphine administered in Nutella® were demonstrated. This was evident at doses of 1.0 mg/kg 60 and 120 min post administration (P Nutella® in a 10-fold higher dose, as well as approximately 60 min earlier, than with the more commonly employed subcutaneous route of administration.

  18. Topography of Venus and earth - A test for the presence of plate tectonics

    Science.gov (United States)

    Head, J. W.; Yuter, S. E.; Solomon, S. C.

    1981-01-01

    Comparisons of earth and Venus topography by use of Pioneer/Venus radar altimetry are examined. Approximately 93% of the Venus surface has been mapped with a horizontal resolution of 200 km and a vertical resolution of 200 m. Tectonic troughs have been indicated in plains regions which cover 65% of Venus, and hypsometric comparisons between the two planets' elevation distributions revealed that while the earth has a bimodal height distribution, Venus displays a unimodal configuration, with 60% of the planet surface within 500 m of the modal planet radius. The effects of mapping the earth at the same resolution as the Venus observations were explored. Continents and oceans were apparent, and although folded mountains appeared as high spots, no indications of tectonic activity were discernible. A NASA Venus Orbiting Imaging radar is outlined, which is designed to detect volcanoes, folded mountain ranges, craters, and faults, and thereby allow definition of possible plate-tectonic activity on Venus.

  19. Topography of Venus and earth - A test for the presence of plate tectonics

    Science.gov (United States)

    Head, J. W.; Yuter, S. E.; Solomon, S. C.

    1981-01-01

    Comparisons of earth and Venus topography by use of Pioneer/Venus radar altimetry are examined. Approximately 93% of the Venus surface has been mapped with a horizontal resolution of 200 km and a vertical resolution of 200 m. Tectonic troughs have been indicated in plains regions which cover 65% of Venus, and hypsometric comparisons between the two planets' elevation distributions revealed that while the earth has a bimodal height distribution, Venus displays a unimodal configuration, with 60% of the planet surface within 500 m of the modal planet radius. The effects of mapping the earth at the same resolution as the Venus observations were explored. Continents and oceans were apparent, and although folded mountains appeared as high spots, no indications of tectonic activity were discernible. A NASA Venus Orbiting Imaging radar is outlined, which is designed to detect volcanoes, folded mountain ranges, craters, and faults, and thereby allow definition of possible plate-tectonic activity on Venus.

  20. Effect of Cold Agglutination to the Results of Routine Blood Test and Its Related Schemes Dealing with These Interferences%冷凝集对血常规检测结果的影响及处理方案探讨

    Institute of Scientific and Technical Information of China (English)

    徐健; 周道银; 俞靖龙; 唐古生

    2011-01-01

    目的 探讨血常规检验中冷凝集现象的处理方案,以获取简单易行、纠正效果理想的方案.方法 将15例确定为冷凝集的标本,分别采用37℃水浴10 min保温、37℃水浴30 min保温、37℃水浴30 min后低温延迟1 min、血浆置换1次和血浆置换3次等不同方案处理后,在SYSMEX XE-2100上检测.以及时重新采血立即检测结果作为"真实值"进行参照,其余各方案与之比对.结果 冷凝集标本检测时,仪器出现红细胞凝集相关报警,RBC和HCT显著减低,MCH和MCHC异常增高,HGB与RBC比例明显异常.重新采血立即检测,相应报警消失,各参数结果恢复正常,涂片未见红细胞凝集.37℃水浴30 min方案各参数检测结果与"真实值"对比均恢复正常(P>0.05);血浆置换3次方案,WBC,RBC,HGB和MCHC恢复正常(P>0.05),但PLT明显降低(P0. 05). The results of WBC,RBC,HGB and MCHC could be corrected by plasma exchange for 3 times (P>0. 05) ,but this method could lead to significantly reduced counts of PLT ( P<0. 05). Other schemes could not totally correct the counts of RBC in part of specimens. Furthermore,agglutinated RBC could still be observed under the microscope if samples were not detected immediately after water bath at 37°C for 30 minutes. Conclusion The best method of dealing with cold agglutinated is to detect the specimen immediately after the blood is drawn again. Secondly,the most effective way of correcting the results of agglutinated specimens is to incubate them at 37°C for 30 minutes before being measured without any delay. An immediately blood smear is also suggested so as to exclude the existence of RBC agglutination completely. An alternative way is to exchange plasma for 3 times if previous method could not totally correct the wrong results m cold agglutinated specimens. However,low counts of PLT could be a defect of this method, and in this condition, counts of PLT should be measured after water bath.

  1. Agglutinated foraminifera from the Ludlow (Silurian) of Ireland

    Science.gov (United States)

    Kaminski, Michael; Ferretti, Annalisa; Messori, Fabio; Papazzoni, Cesare Andrea; Sevastopulo, George

    2017-04-01

    Agglutinated foraminifera are one of the most primitive groups of foraminifera, possibly already appearing in the Cryogenian but usually rare in lower Paleozoic rocks. Their mean standing diversity slowly increased during Cambrian and Ordovician times, reaching a stable value of about 50 genera in the mid-Silurian which remained fairly constant up to the Triassic. An assemblage of agglutinated foraminifera was unexpectedly found in conodont residue from material collected in the Dingle Peninsula, County Kerry, southwestern Ireland. This material comes from rare calcareous occurrences in volcanoclastics previously known for their rich trilobite and conodont assemblages. The limestones are trilobite-crinoidal silty wackestone to packstone, with local brachiopod concentrations, documenting brachiopod-trilobite-crinoidal dominated communities of shallow and well-ventilated water that might have periodically colonized the bottom intercalating with volcanic events and then successively redeposited in deeper waters. The conodont fauna indicates an early Ludlow (Gorstian-earliest Ludfordian) age (Kaminski et al., 2016). The foraminiferal assemblage has limited potential for stratigraphical correlation as long-range taxa are present, but it represents the first record from the Silurian of Ireland. The assemblage is dominated by tubothalamids (Rectoammodiscus and rare Sansabaina), with less abundant monothalamids (Psammosiphonella and Psammosphaera). The assemblage displays low diversity compared with other assemblages described from the British Isles (Kircher & Brasier, 1989). At the species level, this assemblage is identical to those described previously from the Silurian of North America but with lower diversity. Only Rectoammodiscus diai had apparently a wider geographic distribution, including not only the central USA (Oklahoma and Kansas) but also the Welsh Borderlands and Senegal. The affinities with the assemblages reported at several localities in the central

  2. [Identification of mosquitoes' human food source by using the co-agglutination technique].

    Science.gov (United States)

    Castex, M; Fachado, A; Fonte, L

    1997-01-01

    The utilization of a coagglutination technique for the identification of a human source for feeding mosquitoes is described. The dilution of ingested blood samples in filter paper was performed in 2 mL of a sodium chloride solution at 0.85%. It was used a suspension of sensibilized Staphylococcus aureus with rabbit's serum, human plasmatic anti-proteins, and human anti-IgG rabbit's serum discriminated well between human and non human blood. No agglutination was observed with the negative control. This technique proved to be sensitive to identify 100% of the human blood samples taken to the paper 24 hours after the mosquitoes completed their feeding at a temperature of 26 to 28 degrees C. Among mosquitoes fed and collected in the fields the test had a satisfactory result. Therefore, it may be used in routine work in the fields. The results showed the sensitivity and specificity of this method for identifying human blood ingested by mosquitoes.

  3. Analysis of the discontinuous Petrov-Galerkin method with optimal test functions for the Reissner-Mindlin plate bending model

    KAUST Repository

    Calo, Victor M.

    2014-01-01

    We analyze the discontinuous Petrov-Galerkin (DPG) method with optimal test functions when applied to solve the Reissner-Mindlin model of plate bending. We prove that the hybrid variational formulation underlying the DPG method is well-posed (stable) with a thickness-dependent constant in a norm encompassing the L2-norms of the bending moment, the shear force, the transverse deflection and the rotation vector. We then construct a numerical solution scheme based on quadrilateral scalar and vector finite elements of degree p. We show that for affine meshes the discretization inherits the stability of the continuous formulation provided that the optimal test functions are approximated by polynomials of degree p+3. We prove a theoretical error estimate in terms of the mesh size h and polynomial degree p and demonstrate numerical convergence on affine as well as non-affine mesh sequences. © 2013 Elsevier Ltd. All rights reserved.

  4. In Vitro Dissolution Tests of Plutonium and Americium Containing Contamination Originating From ZPPR Fuel Plates

    Energy Technology Data Exchange (ETDEWEB)

    William F. Bauer; Brian K. Schuetz; Gary M. Huestis; Thomas B. Lints; Brian K. Harris; R. Duane Ball; Gracy Elias

    2012-09-01

    Assessing the extent of internal dose is of concern whenever workers are exposed to airborne radionuclides or other contaminants. Internal dose determinations depend upon a reasonable estimate of the expected biological half-life of the contaminants in the respiratory tract. One issue with refractory elements is determining the dissolution rate of the element. Actinides such as plutonium (Pu) and Americium (Am) tend to be very refractory and can have biological half-lives of tens of years. In the event of an exposure, the dissolution rates of the radionuclides of interest needs to be assessed in order to assign the proper internal dose estimates. During the November 2011 incident at the Idaho National Laboratory (INL) involving a ZPPR fuel plate, air filters in a constant air monitor (CAM) and a giraffe filter apparatus captured airborne particulate matter. These filters were used in dissolution rate experiments to determine the apparent dissolution half-life of Pu and Am in simulated biological fluids. This report describes these experiments and the results. The dissolution rates were found to follow a three term exponential decay equation. Differences were noted depending upon the nature of the biological fluid simulant. Overall, greater than 95% of the Pu and 93% of the Am were in a very slow dissolving component with dissolution half-lives of over 10 years.

  5. Invited article: a test-facility for large-area microchannel plate detector assemblies using a pulsed sub-picosecond laser.

    Science.gov (United States)

    Adams, Bernhard; Chollet, Matthieu; Elagin, Andrey; Oberla, Eric; Vostrikov, Alexander; Wetstein, Matthew; Obaid, Razib; Webster, Preston

    2013-06-01

    The Large Area Picosecond Photodetector Collaboration is developing large-area fast photodetectors with time resolution tests on bare 8 in.-square MCP plates or into a smaller chamber for tests on 33-mm circular substrates. We present the experimental setup, detector calibration, data acquisition, analysis tools, and typical results demonstrating the performance of the test facility.

  6. An early Cambrian agglutinated tubular lophophorate with brachiopod characters

    Science.gov (United States)

    Zhang, Z.-F.; Li, G.-X.; Holmer, L. E.; Brock, G. A.; Balthasar, U.; Skovsted, C. B.; Fu, D.-J.; Zhang, X.-L.; Wang, H.-Z.; Butler, A.; Zhang, Z.-L.; Cao, C.-Q.; Han, J.; Liu, J.-N.; Shu, D.-G.

    2014-05-01

    The morphological disparity of lophotrochozoan phyla makes it difficult to predict the morphology of the last common ancestor. Only fossils of stem groups can help discover the morphological transitions that occurred along the roots of these phyla. Here, we describe a tubular fossil Yuganotheca elegans gen. et sp. nov. from the Cambrian (Stage 3) Chengjiang Lagerstätte (Yunnan, China) that exhibits an unusual combination of phoronid, brachiopod and tommotiid (Cambrian problematica) characters, notably a pair of agglutinated valves, enclosing a horseshoe-shaped lophophore, supported by a lower bipartite tubular attachment structure with a long pedicle with coelomic space. The terminal bulb of the pedicle provided anchorage in soft sediment. The discovery has important implications for the early evolution of lophotrochozoans, suggesting rooting of brachiopods into the sessile lophotrochozoans and the origination of their bivalved bauplan preceding the biomineralization of shell valves in crown brachiopods.

  7. Sperm-agglutinating antibodies and testicular morphology in fifty-nine men with azoospermia or cryptozoospermia.

    Science.gov (United States)

    Friberg, J; Kjessler, B

    1975-04-01

    The relationship between the state of the germinal epithelium and the type and titer of circulating sperm-agglutinating antibodies has been investigated in a series of 59 azoospermic or occasionally cryptozoospermic men. The patients were grouped according to the condition of the germinal epithelium as observed from testicular biopsy specimens, as well as to type and titer of circulating sperm-agglutinating antibodies investigated by a previously described microagglutination technique. Evidence is presented to suggest that the presence of mature spermatozoa in the testicular structures may be a prerequisite for the spontaneous production of circulating sperm-agglutinating antibodies, at least of the head-to-tail (H-T) agglutinating type. Furthermore, these circulating H-T sperm-agglutinating antibodies, once they are formed, do not seem to interfere adversely with the germinal epithelium of the carrier.

  8. Two-Dimensional Mapping of the Calculated Fission Power for the Full-Size Fuel Plate Experiment Irradiated in the Advanced Test Reactor

    Science.gov (United States)

    Chang, G. S.; Lillo, M. A.

    2009-08-01

    The National Nuclear Security Administrations (NNSA) Reduced Enrichment for Research and Test Reactors (RERTR) program assigned to the Idaho National Laboratory (INL) the responsibility of developing and demonstrating high uranium density research reactor fuel forms to enable the use of low enriched uranium (LEU) in research and test reactors around the world. A series of full-size fuel plate experiments have been proposed for irradiation testing in the center flux trap (CFT) position of the Advanced Test Reactor (ATR). These full-size fuel plate tests are designated as the AFIP tests. The AFIP nominal fuel zone is rectangular in shape having a designed length of 21.5-in (54.61-cm), width of 1.6-in (4.064-cm), and uniform thickness of 0.014-in (0.03556-cm). This gives a nominal fuel zone volume of 0.482 in3 (7.89 cm3) per fuel plate. The AFIP test assembly has two test positions. Each test position is designed to hold 2 full-size plates, for a total of 4 full-size plates per test assembly. The AFIP test plates will be irradiated at a peak surface heat flux of about 350 W/cm2 and discharged at a peak U-235 burn-up of about 70 at.%. Based on limited irradiation testing of the monolithic (U-10Mo) fuel form, it is desirable to keep the peak fuel temperature below 250°C to achieve this, it will be necessary to keep plate heat fluxes below 500 W/cm2. Due to the heavy U-235 loading and a plate width of 1.6-in (4.064-cm), the neutron self-shielding will increase the local-to-average-ratio (L2AR) fission power near the sides of the fuel plates. To demonstrate that the AFIP experiment will meet the ATR safety requirements, a very detailed 2-dimensional (2D) Y-Z fission power profile was evaluated in order to best predict the fuel plate temperature distribution. The ability to accurately predict fuel plate power and burnup are essential to both the design of the AFIP tests as well as evaluation of the irradiated fuel performance. To support this need, a detailed MCNP Y

  9. First joint test beam of CMS Drift Tubes (DT) and Resistive Plate Chambers (RPC)

    CERN Multimedia

    Paolo Giacomelli

    2001-01-01

    The first full size muon drift tube chamber ever built for the CMS barrel with the final cell design (constructed at CIEMAT, Madrid) was succesfully tested with a muon beam in September 2001 at the Gamma Irradiation Facility (GIF) at CERN. For the first time also both muon detectors for the CMS barrel (DT + RPC) were coupled together. The results of this test were fully succesful and confirmed the excellent performance of both detectors together in a radiation environment.

  10. Tests and Evaluation of Upgraded Flat-Plate and Waffle-Slab Floor Systems

    Science.gov (United States)

    1983-12-01

    tested in the LBLG. The LBLG (Figure 2.J9) is a large, three-dimensional device designed pri- marily to test underground protective structures...34 Report AIWL-TR-74-102, Octo- ber 1974, Air Force Weapons Laboratory, Kirtland Air Force Base, N. Mex . 9. M. E. Criswell; "Strength and Behavior of...35th St. Commander, Air Force Weapons Laboratory, AFSC Chicago, Ill. 60616 ATTN: SUL, Technical Library Kirtland Air Force Base, N. Mex . 87117 Director

  11. Imaging based agglutination measurement of magnetic micro-particles on a lab-on-a-disk platform

    DEFF Research Database (Denmark)

    Wantiya, P.; Burger, Robert; Alstrøm, Tommy Sonne;

    2014-01-01

    In this work we present a magnetic micro beads based agglutination assay on a centrifugal microfluidic platform. An imaging based method is used to quantify bead agglutination and measure the concentration of antibodies or C-reactive protein in solution.......In this work we present a magnetic micro beads based agglutination assay on a centrifugal microfluidic platform. An imaging based method is used to quantify bead agglutination and measure the concentration of antibodies or C-reactive protein in solution....

  12. An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation.

    Directory of Open Access Journals (Sweden)

    Ying Zhang

    Full Text Available BACKGROUND: Integrins are a family of adhesion receptors which regulate cell proliferation, differentiation, leukocyte migration, and complement receptor-dependent phagocytosis. In invertebrates, as a cell adhesion receptor, β integrins play an important role for the balanced activation of immune defense responses especially during the encounter of infections. The present study attempts to characterize the immune functions of shrimp integrin (LvIntegrin to have better understanding on the immune system and its regulation mechanisms in shrimps. METHODOLOGY: A shrimp integrin was identified from the Pacific white shrimp Litopenaeus vannamei (designated as LvIntegrin. Its full-length cDNA was of 2621 bp with an open reading frame (ORF of 2439 bp encoding a polypeptide of 812 amino acids. The mRNA expression of LvIntegrin was significantly up-regulated at 3, 6 and 12 h after Listonella anguillarum challenge. The cDNA fragment encoding β integrin domains (βA and hybrid domain of LvIntegrin was recombined and expressed in Escherichia coli BL21(DE3-pLysS. The recombinant protein (rLvIntegrin could significantly agglutinate the tested microbe including E. coli JM109, L. anguillarum, Micrococcus luteus and Candida dattiladattila in the presence of divalent cations. Moreover, when NIH3T3 cells were cultured with rLvIntegrin, the proliferation rate increased significantly in a dose-dependent manner. CONCLUSIONS: LvIntegrin, a shrimp β integrin was identified from L. vannamei, shared several highly conserved features. LvIntegrin exhibited broad-spectrum agglutination activity towards both bacteria and fungi and could improve the proliferation of NIH3T3 cells, indicating that LvIntegrin is involved in the immune response against microbe challenge and regulation of cell proliferation as a cell adhesion receptor in shrimp.

  13. Investigation of Thermal Performance of Flat Plate and Evacuated Tubular Solar Collectors According to a New Dynamic Test Method

    DEFF Research Database (Denmark)

    Kong, Weiqiang; Wang, Zhifeng; Fan, Jianhua;

    2012-01-01

    obtain fluid thermal capacitance in data processing. Then theoretical analysis and experimental verification are carried out to investigate influencing factors of obtaining accurate and stable second order term. A flat plate and ETC solar collector are compared using both the new dynamic method......A new dynamic test method is introduced. This so called improved transfer function method features on two new collector parameters. One is time term which can indicate solar collector's inner heat transfer ability and the other is a second order term of collector mean fluid temperature which can...... and a standard method. The results show that the improved function method can accurately and robustly estimate these two kinds of solar collectors....

  14. Defect Localization Capabilities of a Global Detection Scheme: Spatial Pattern Recognition Using Full-field Vibration Test Data in Plates

    Science.gov (United States)

    Saleeb, A. F.; Prabhu, M.; Arnold, S. M. (Technical Monitor)

    2002-01-01

    Recently, a conceptually simple approach, based on the notion of defect energy in material space has been developed and extensively studied (from the theoretical and computational standpoints). The present study focuses on its evaluation from the viewpoint of damage localization capabilities in case of two-dimensional plates; i.e., spatial pattern recognition on surfaces. To this end, two different experimental modal test results are utilized; i.e., (1) conventional modal testing using (white noise) excitation and accelerometer-type sensors and (2) pattern recognition using Electronic speckle pattern interferometry (ESPI), a full field method capable of analyzing the mechanical vibration of complex structures. Unlike the conventional modal testing technique (using contacting accelerometers), these emerging ESPI technologies operate in a non-contacting mode, can be used even under hazardous conditions with minimal or no presence of noise and can simultaneously provide measurements for both translations and rotations. Results obtained have clearly demonstrated the robustness and versatility of the global NDE scheme developed. The vectorial character of the indices used, which enabled the extraction of distinct patterns for localizing damages proved very useful. In the context of the targeted pattern recognition paradigm, two algorithms were developed for the interrogation of test measurements; i.e., intensity contour maps for the damaged index, and the associated defect energy vector field plots.

  15. Standardized performance tests of collectors of solar thermal energy-a flat-plate collector with a single-tube serpentine flow distribution

    Science.gov (United States)

    Johnson, S.

    1976-01-01

    This preliminary data report gives basic test results of a flat-plate solar collector whose performance was determined in the NASA-Lewis solar simulator. The collector was tested over ranges of inlet temperatures, fluxes and coolant flow rates. Collector efficienty is correlated in terms of inlet temperature and flux level.

  16. Identification and agglutination properties of hemocyanin from the mud crab (Scylla serrata).

    Science.gov (United States)

    Yan, Fang; Zhang, Yueling; Jiang, Ruiping; Zhong, Mingqi; Hu, Zhong; Du, Hong; Lun, Jingsheng; Chen, Jiehui; Li, Yuanyou

    2011-01-01

    Infectious diseases have significantly delayed the growth of crab aquaculture. Identification of the immune molecules and characterization of the defense mechanisms will be pivotal to the reduction of these diseases. Hemocyanin is an important non-specific immune protein present in the hemolymph of both mollusks and arthropods. However, little is known about the hemocyanin from the mud crab Scylla serrata. In this study, we identified the S. serrata hemocyanin using affinity proteomics and investigated its agglutinative properties. The results showed that S. serrata hemocyanin consists of five subunits with molecular weights of 70, 72, 75, 76 and 80 kDa, respectively. It demonstrated agglutination activities against seven bacterial species at concentrations ranging from 7.5 to 30 μg/ml. Agglutination was inhibited by 50-200 mM of N-acetylneuraminic acid, α-d-glucose, d-galactose and d-xylose. The 76 kDa subunit was identified as the protein that primarily binds bacterial cells and we speculate that it functions as the agglutinating subunit. We showed that outer membrane proteins (Omp) of bacteria could completely inhibit agglutination and that the agglutination activities of hemocyanin against Escherichia coli ▵OmpA and ▵OmpX mutants were significantly decreased, suggesting that these two Omps may be important ligands of hemocyanin. Together, the data collectively suggests that the 76 kDa subunit of S. serrata hemocyanin mediates agglutination through recognition of OmpA and OmpX proteins in bacteria.

  17. Laboratory Test for Measurement of Adhesion Strength of Spray Ice to Coated Flat Plates

    Science.gov (United States)

    1990-05-01

    became trapped within the ice. After the by test group 612 during icing due to nozzle freezeup sprayer was turned off, the unfrozen water became and the...indicated if and when nozzle freezeup occurred. Short and the push block increased as the block moved for- duration peaks in the nozzle temperature occurred...either nozzle freezeup and conse- bond strength of each sample. Notice, in Figure 2 1. the quent efforts to thaw, the application of more insulation

  18. Critical assessment of precracked specimen configuration and experimental test variables for stress corrosion testing of 7075-T6 aluminum alloy plate

    Science.gov (United States)

    Domack, M. S.

    1985-01-01

    A research program was conducted to critically assess the effects of precracked specimen configuration, stress intensity solutions, compliance relationships and other experimental test variables for stress corrosion testing of 7075-T6 aluminum alloy plate. Modified compact and double beam wedge-loaded specimens were tested and analyzed to determine the threshold stress intensity factor and stress corrosion crack growth rate. Stress intensity solutions and experimentally determined compliance relationships were developed and compared with other solutions available in the literature. Crack growth data suggests that more effective crack length measurement techniques are necessary to better characterize stress corrosion crack growth. Final load determined by specimen reloading and by compliance did not correlate well, and was considered a major source of interlaboratory variability. Test duration must be determined systematically, accounting for crack length measurement resolution, time for crack arrest, and experimental interferences. This work was conducted as part of a round robin program sponsored by ASTM committees G1.06 and E24.04 to develop a standard test method for stress corrosion testing using precracked specimens.

  19. Simulation analysis of impact tests of steel plate reinforced concrete and reinforced concrete slabs against aircraft impact and its validation with experimental results

    Energy Technology Data Exchange (ETDEWEB)

    Sadiq, Muhammad, E-mail: muhammad.sadiq@pnra.org [Center for Nuclear Safety (CNS), Pakistan Nuclear Regulatory Authority (PNRA), PO Box 1912, Islamabad (Pakistan); Xiu Yun, Zhu, E-mail: lyzhuxiuyun@163.com [Nuclear and Radiation Safety Center (NSC), Ministry of Environmental Protection (MEP), Beijing (China); Rong, Pan, E-mail: panrong@chinansc.cn [Nuclear and Radiation Safety Center (NSC), Ministry of Environmental Protection (MEP), Beijing (China)

    2014-07-01

    Highlights: • Simulation analysis is carried out with two constitutive concrete models. • Winfrith model can better simulate nonlinear response of concrete than CSCM model. • Performance of steel plate concrete is better than reinforced concrete. • Thickness of safety related structures can be reduced by adopting steel plates. • Analysis results, mainly concrete material models should be validated. - Abstract: The steel plate reinforced concrete and reinforced concrete structures are used in nuclear power plants for protection against impact of an aircraft. In order to compare the impact resistance performance of steel plate reinforced concrete and reinforced concrete slabs panels, simulation analysis of 1/7.5 scale model impact tests is carried out by using finite element code ANSYS/LS-DYNA. The damage modes of all finite element models, velocity time history curves of the aircraft engine and damage to aircraft model are compared with the impact test results of steel plate reinforced concrete and reinforced concrete slab panels. The results indicate that finite element simulation results correlate well with the experimental results especially for constitutive winfrith concrete model. Also, the impact resistance performance of steel plate reinforced concrete slab panels is better than reinforced concrete slab panels, particularly the rear face steel plate is very effective in preventing the perforation and scabbing of concrete than conventional reinforced concrete structures. In this way, the thickness of steel plate reinforced concrete structures can be reduced in important structures like nuclear power plants against impact of aircraft. It also demonstrates the methodology to validate the analysis procedure with experimental and analytical studies. It may be effectively employed to predict the precise response of safety related structures against aircraft impact.

  20. Some tests of flat plate photovoltaic module cell temperatures in simulated field conditions

    Science.gov (United States)

    Griffith, J. S.; Rathod, M. S.; Paslaski, J.

    1981-01-01

    The nominal operating cell temperature (NOCT) of solar photovoltaic (PV) modules is an important characteristic. Typically, the power output of a PV module decreases 0.5% per deg C rise in cell temperature. Several tests were run with artificial sun and wind to study the parametric dependencies of cell temperature on wind speed and direction and ambient temperature. It was found that the cell temperature is extremely sensitive to wind speed, moderately so to wind direction and rather insensitive to ambient temperature. Several suggestions are made to obtain data more typical of field conditions.

  1. Assessment of the Resistance of a Polymethylmethacrylate (Pmma Dynamic Compression Plate (DCP Prototype Tested on Osteotomized Canine Femurs

    Directory of Open Access Journals (Sweden)

    Leonel Cardona R.

    2011-06-01

    Full Text Available Femur fractures are the most frequent long bone fractures in small animals. Due to the femur’s anatomical position, it is difficult to immobilize, and therefore internal fixations are very useful. Dynamic compression plates (DCP provide high stability, are durable, minimize fragment movement and promote primary healing of the bone. Advantages of this treatment include anatomical reconstruction, early mobility and carrying capacity of the affected limb. Polymethylmethacrylate (PMMA is an acrylic resin that has proved very useful in bone tumor treatment, cranial deformation prosthesis, percutaneous vertebroplasty and testicular prosthesis in animals. The purpose of this study was to manufacture DCP-PMMA and test its resistance to the different forces present in a fracture. Forty-eight (48 3.5MM x 4-hole DCP were made from an alginate mold. Six (6 femurs were obtained from canine cadavers zeighing from 10 to 20 kg, which underwent osteotomy simulating an oblique fracture. The plates were subsequently positioned in the osteotomized bones to submit them to the various forces involved in a fracture. The DCP-PMMA resisted a torque force of 2.83 newton/metres and a compression and flexion force of 0.21 kilonewton. The obtained resistance of the DCP-PMMA was an average of 20 kg per force. The results of this study show that it is possible to make a DCP-PMMA 3.5 mm x 4-hole, the resistance of which is of 20 kg against the three applied forces, and that it can be used to stabilize long bone fractures subjected to a pressure of less than 20 kg.

  2. Testing of Ni-plated ferritic steel interconnect in SOFC stacks

    DEFF Research Database (Denmark)

    Nielsen, K.A.; Dinesen, A.R.; Korcakova, L.

    2006-01-01

    Stack tests were run at 850 °C for periods from 80 hours to 1,150 hours to develop contacting procedures and at the same time evaluate the performance of a 5 μm electroplated nickel coating on a ferritic Fe22Cr interconnect. The metallic nickel coating reacted relatively quickly during the initial...... heating to 1,030 °C. During this time, 20–70 μm thick surface layers of austenitic steel were formed, which were covered by a 1–4 μm chromia layer on the anode side and by a layer of mixed Cr-Fe-Ni-spinels over a 1–4 μm chromia layer on the cathode side. The microstructure and composition...

  3. Status of the NASA-Lewis flat-plate collector tests with a solar simulator

    Science.gov (United States)

    Simon, F. F.

    1974-01-01

    Simulator test results of 15 collector types are reported. Collectors are given performance ratings according to their use for pool heating, hot water, absorption A/C or heating and solar Rankine machines. Collectors found to be good performers in the above categories, except for pool heating, were a black nickel coated, 2 glass collector, and a black paint 2 glass collector containing a mylar honeycomb. For pool heating, a black paint, one glass collector was found to be the best performer. Collector performance parameters of 5 collector types were determined to aid in explaining the factors that govern performance. The two factors that had the greatest effect on collector performance were the collector heat loss and the coating absorptivity.

  4. Geo-energy Test Beds: part of the European Plate Observing System

    Science.gov (United States)

    Stephenson, Michael; Schofield, David; Luton, Christopher; Haslinger, Florian; Henninges, Jan; Giardini, Domenico

    2016-04-01

    For 2020, the EU has committed to cutting its greenhouse gas emissions to 20% below 1990 levels and further cuts are being decided for 2050. This commitment is one of the headline targets of the Europe 2020 growth strategy and is being implemented through binding legislation. This decarbonisation of the EU economy is one dimension of an overall EU energy and climate framework that is mutually interlinked with the need to ensure energy security, promote a fully integrated energy market, promote energy efficiency and promote research innovation and competitiveness. Power generation will have to take a particularly large part in emissions reductions (-54 to -68% by 2030 and -93 to -99% by 2050), mainly by focussing on increasing surface renewables (wind, tidal and solar) but also on carbon capture and storage on fossil fuel and biofuel power plants, shale gas, nuclear and geothermal power. All the above generation technologies share common geological challenges around containment, safety and environmental sustainability. In a densely populated continent, this means that high levels of subsurface management are needed to fully realise the energy potential. In response to this need, across Europe, public and private sector funded, experimental test and monitoring facilities and infrastructures (Geo-energy Test Beds, GETB) are being developed. These GETB investigate the processes, technology and practices that facilitate the sustainable exploitation of Geo-energy resources and are of intense interest to the public and regulators alike. The vision of EPOS IP Work Package 17 (wp17) is to promote research and innovation in Geo-energy that reflects core European energy priorities through provision of virtual access to data and protocols and trans-national access to GETB experiments. This will be achieved through provision of access to continuous strategic observations, promotion of the integrated use of data and models from European GETB, development of underpinning research

  5. Biocompatibility Analyses of Al₂O₃-Treated Titanium Plates Tested with Osteocyte and Fibroblast Cell Lines.

    Science.gov (United States)

    Smargiassi, Alberto; Bertacchini, Jessika; Checchi, Marta; Cavani, Francesco; Ferretti, Marzia; Palumbo, Carla

    2017-06-16

    Osseointegration of a titanium implant is still an issue in dental/orthopedic implants durable over time. The good integration of these implants is mainly due to their surface and topography. We obtained an innovative titanium surface by shooting different-in-size particles of Al₂O₃ against the titanium scaffolds which seems to be ideal for bone integration. To corroborate that, we used two different cell lines: MLO-Y4 (murine osteocytes) and 293 (human fibroblasts) and tested the titanium scaffolds untreated and treated (i.e., Al₂O₃ shot-peened titanium surfaces). Distribution, density, and expression of adhesion molecules (fibronectin and vitronectin) were evaluated under scanning electron microscope (SEM) and confocal microscope (CM). DAPI and fluorochrome-conjugated antibodies were used to highlight nuclei, fibronectin, and vitronectin, under CM; cell distribution was analyzed after gold-palladium sputtering of samples by SEM. The engineered biomaterial surfaces showed under SEM irregular morphology displaying variously-shaped spicules. Both SEM and CM observations showed better outcome in terms of cell adhesion and distribution in treated titanium surfaces with respect to the untreated ones. The results obtained clearly showed that this kind of surface-treated titanium, used to manufacture devices for dental implantology: (i) is very suitable for cell colonization, essential prerequisite for the best osseointegration, and (ii) represents an excellent solution for the development of further engineered implants with the target to obtain recovery of stable dental function over time.

  6. The Cenozoic Diversity of Agglutinated Foraminifera - Evidence for a late Oligocene to early Miocene diversification event

    Science.gov (United States)

    Kaminski, Michael; Setoyama, Eiichi; Kender, Sev; Cetean, Claudia

    2014-05-01

    The agglutinated foraminifera are among the most abundant micro-organisms in the deep marine environment and have a diversity record extending back to the late Precambrian. We present an updated diversity curve for agglutinated foraminiferal genera based on the stratigraphic ranges of all the agglutinated genera recognized as valid in the classification of Kaminski (2014). The data set for this analysis is based on the stratigraphic ranges of agglutinated genera published in Foraminiferal Genera and their Classification, which has been subsequently updated based on published studies and our new observations. The mean standing diversity of agglutinated foraminiferal genera was compiled by counting the number of boundary crossers rather than the number of genera in each stage. In this study, we report the stratigraphic and geographical occurrence of a benthic foraminiferal diversification event that has previously received little attention. In the latest Oligocene to earliest Miocene a number of trochospiral agglutinated genera with alveolar or canaliculate walls first appeared in the fossil record. Our studies of late Oligocene of the Congo fan, offshore Angola (Kender et al., 2008; Cetean and Kaminski, 2011) have revealed a diverse assemblage that includes new taxa of deep-water agglutinated foraminifera. In a biostratigraphic study of the Miocene foraminiferal assemblages Kender et al. (2008) noted steadily increasing diversity and proportions of infaunal agglutinated foraminiferal morphotypes over the lower Miocene interval. The proportion of infaunal agglutinated foraminifera assigned to the order Textularida increased dramatically in the lower mid-Miocene, suggesting expansion of the oxygen minimum zone into deeper waters. In addition to the trochospiral alveolar genera, several species of Reticulophragmium and Cyclammina display rapid diversification into numerous separate lineages that are at present not reflected in our generic diversity record owing to

  7. Assessment of a 96-Well Plate Assay of Quantitative Drug Susceptibility Testing for Mycobacterium Tuberculosis Complex in China

    Science.gov (United States)

    Xia, Hui; Zheng, Yang; Zhao, Bing; van den Hof, Susan; Cobelens, Frank; Zhao, YanLin

    2017-01-01

    Objective To evaluate the performance of the Sensitire MYCOTB MIC Plate (MYCOTB) which could measure the twelve anti-tuberculosis drugs susceptibility on one 96-wells plate. Methods A total of 140 MDR-TB strains and 60 non-MDR strains were sub-cultured and 193 strains were finally tested for drug resistance using MYCOTB and agar proportion method (APM) and another 7 strains failed of subculture. The drugs included ofloxacin (Ofx), moxifloxacin (Mfx), rifampin (RFP), amikacin (Am), rifabutin (Rfb), para-aminosalicylic acid (PAS), ethionamide (Eth), isoniazid (INH), kanamycin (Km), ethambutol (EMB), streptomycin (Sm), and cycloserine(Cs). The categorical agreement, conditional agreement, sensitivity and specificity of MYCOTB were assessed in comparison with APM. For strains with inconsistent results between MYCOTB and APM, the drug resistance related gene fragments were amplified and sequenced: gyrA for Ofx and Mfx; rpoB for RFP and Rfb; embB for EMB; rpsl for Sm; katG and the promoter region of inhA for INH, ethA and the promoter region of inhA for Eth. The sequence results were compared with results of MYCOTB and APM to analyze the consistency between sequence results and MYCOTB or APM. Results The categorical agreement between two methods for each drug ranged from 88.6% to 100%. It was the lowest for INH (88.6%). The sensitivity and specificity of MYCOTB ranged from 71.4% to 100% and 84.3% to 100%, respectively. The sensitivity was lowest for Cs(71.4%), EMB at 10μg/ml (80.0%) and INH at 10.0μg/ml (84.6%). The specificity was lowest for Rfb (84.3%). Overall discordance between the two phenotypic methods was observed for 96 strains, of which 63 (65.6%) were found susceptible with APM and resistant with MYCOTB and the remaining 33(34.4%) strains were resistant by APM and susceptible with MYCOTB. 34/52 (65.4%) sequenced APM susceptible and MYCOTB resistant(APM-S/MYCOTB-R) strains had mutations or insertions in the amplified regions. 20/30 (66.7%) sequenced APM

  8. Design of High Frequency Induction Plate Forming Test-Bed%高频感应弯板成形试验台设计

    Institute of Scientific and Technical Information of China (English)

    刘守法; 范平

    2012-01-01

    Specific to the present situation of the lack of high frequency induction plate forming experimental equipment at home and abroad, a high frequency induction plate forming test梑ed was designed to conduct high frequency induction plate forming test,which will be helpful to study the law of the high frequency induction plate forming.The finite element analysis for tine plate forming model was conducted with Ansys software and the plate forming test was also completed on the test-bed under the scam parameters After comparing the bending angle curves of the finite element analysis with the experimental,it is observed that the two bending angle curves are basically the same to each other and the bending angle error between the simulation and the test is kss than 12.1%.%针对国内外高频感应弯板成形试验设备缺乏现状,设计了弯板成形试验台,用于进行高频感应弯板成形试验,进而有助于研究高频感应弯板成形规律.利用Ansys软件对弯板成形模型进行了有限元分析,并利用试验台进行了相同参数下板料成形试验,将有限元分析得到的折弯角度曲线与试验得到的实际折弯角度曲线进行了对比,结果表明:有限元分析得到的板料折弯角度曲线与试验得到的板料折弯角度曲线基本相同,二者折弯角度偏差小于12.1%.

  9. Miocene deep water agglutinated foraminifera from Viosca Knoll, offshore Louisiana (Gulf of Mexico)

    OpenAIRE

    Green, R C; Kaminski, M.A.; Sikora, P. J.

    2004-01-01

    An exploration well from the Gulf of Mexico, Amoco Viosca Knoll-915, has been studied in order to document the Neogene foraminiferal assemblages. Ditch cuttings samples from the Amoco V.K. 915 well yielded diverse assemblages of agglutinated and calcareous benthic foraminifera over a stratigraphic interval of 2940 m. Three species associations can be identified in the studied interval; the stratigraphical location of these associations is evident when total agglutinated species...

  10. Preventing Staphylococcus aureus sepsis through the inhibition of its agglutination in blood.

    Science.gov (United States)

    McAdow, Molly; Kim, Hwan Keun; Dedent, Andrea C; Hendrickx, Antoni P A; Schneewind, Olaf; Missiakas, Dominique M

    2011-10-01

    Staphylococcus aureus infection is a frequent cause of sepsis in humans, a disease associated with high mortality and without specific intervention. When suspended in human or animal plasma, staphylococci are known to agglutinate, however the bacterial factors responsible for agglutination and their possible contribution to disease pathogenesis have not yet been revealed. Using a mouse model for S. aureus sepsis, we report here that staphylococcal agglutination in blood was associated with a lethal outcome of this disease. Three secreted products of staphylococci--coagulase (Coa), von Willebrand factor binding protein (vWbp) and clumping factor (ClfA)--were required for agglutination. Coa and vWbp activate prothrombin to cleave fibrinogen, whereas ClfA allowed staphylococci to associate with the resulting fibrin cables. All three virulence genes promoted the formation of thromboembolic lesions in heart tissues. S. aureus agglutination could be disrupted and the lethal outcome of sepsis could be prevented by combining dabigatran-etexilate treatment, which blocked Coa and vWbp activity, with antibodies specific for ClfA. Together these results suggest that the combined administration of direct thrombin inhibitors and ClfA-antibodies that block S. aureus agglutination with fibrin may be useful for the prevention of staphylococcal sepsis in humans.

  11. Multi-channel, probe colorimeter for use with the micr-ELISA test, which makes use of disposable flat-bottom microhemagglutination plates.

    Science.gov (United States)

    McMurray, C H; Blanchflower, W J

    1979-04-01

    We describe a multiprobe colorimeter that permits the reading of enzyme-linked immunosorbent assays (ELISA) in situ, by use of microhemagglutination plates. The use of dipping probes eliminates interference from meniscus effects and air-bubble entrapment. Detailed validation tests have been done and potential sources of inaccuracy identified.

  12. Microstructural Characterization of a Mg Matrix U-Mo Dispersion Fuel Plate Irradiated in the Advanced Test Reactor to High Fission Density: SEM Results

    Science.gov (United States)

    Keiser, Dennis D.; Jue, Jan-Fong; Miller, Brandon D.; Gan, Jian; Robinson, Adam B.; Medvedev, Pavel G.; Madden, James W.; Moore, Glenn A.

    2016-06-01

    Low-enriched (U-235 reactors. In most cases, fuel plates with Al or Al-Si alloy matrices have been tested in the Advanced Test Reactor to support this development. In addition, fuel plates with Mg as the matrix have also been tested. The benefit of using Mg as the matrix is that it potentially will not chemically interact with the U-Mo fuel particles during fabrication or irradiation, whereas with Al and Al-Si alloys such interactions will occur. Fuel plate R9R010 is a Mg matrix fuel plate that was aggressively irradiated in ATR. This fuel plate was irradiated as part of the RERTR-8 experiment at high temperature, high fission rate, and high power, up to high fission density. This paper describes the results of the scanning electron microscopy (SEM) analysis of an irradiated fuel plate using polished samples and those produced with a focused ion beam. A follow-up paper will discuss the results of transmission electron microscopy (TEM) analysis. Using SEM, it was observed that even at very aggressive irradiation conditions, negligible chemical interaction occurred between the irradiated U-7Mo fuel particles and Mg matrix; no interconnection of fission gas bubbles from fuel particle to fuel particle was observed; the interconnected fission gas bubbles that were observed in the irradiated U-7Mo particles resulted in some transport of solid fission products to the U-7Mo/Mg interface; the presence of microstructural pathways in some U-9.1 Mo particles that could allow for transport of fission gases did not result in the apparent presence of large porosity at the U-7Mo/Mg interface; and, the Mg-Al interaction layers that were present at the Mg matrix/Al 6061 cladding interface exhibited good radiation stability, i.e. no large pores.

  13. A small ciliary surface glycoprotein of the monogenean parasite Neobenedenia girellae acts as an agglutination/immobilization antigen and induces an immune response in the Japanese flounder Paralichthys olivaceus.

    Science.gov (United States)

    Hatanaka, A; Umeda, N; Yamashita, S; Hirazawa, N

    2005-11-01

    The capsalid monogenean Neobenedenia girellae, a parasite of seawater fishes, was found to express an antigen that elicits antibodies in rabbits, and these antibodies had agglutination/immobilization activity against N. girellae larvae (oncomiracidia) in vitro. Indirect immunofluorescence staining of N. girellae oncomiracidia showed that this agglutination/immobilization antigen was expressed on the surface of cilia. An intraperitoneal injection of ciliary proteins (either sonicated or intact) with adjuvant also elicited agglutinizing/immobilizing antibodies in sera from Japanese flounder, Paralichthys olivaceus. These antisera showed a clear correlation between anti-ciliary antibody levels (measured by enzyme-linked immunosorbent assays) and their agglutination/immobilization activity. Anti-ciliary antibody levels in Japanese flounder reached a plateau at 39 days after booster immunization and were significantly higher in the two immunized groups (injection of sonicated or intact cilia) as compared with control fish (injection of bovine serum albumin; ANOVA, Tukey's test, P agglutination/immobilization antigen based on SDS-polyacrylamide gel electrophoresis and immunoblot analyses with rabbit and fish antisera.

  14. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci.

    Science.gov (United States)

    Stepanović, Srdjan; Vuković, Dragana; Hola, Veronika; Di Bonaventura, Giovanni; Djukić, Slobodanka; Cirković, Ivana; Ruzicka, Filip

    2007-08-01

    The details of all steps involved in the quantification of biofilm formation in microtiter plates are described. The presented protocol incorporates information on assessment of biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assaying biofilm production. The obtained results should simplify quantification of biofilm formation in microtiter plates, and make it more reliable and comparable among different laboratories.

  15. Diagnóstico precoce das faringoamigdalites estreptocócicas: avaliação pelo teste de aglutinação de partículas de látex Early diagnosis of streptococcal pharyngotonsillitis: assessment by latex particle agglutination test

    Directory of Open Access Journals (Sweden)

    Maria Jussara F. Fontes

    2007-10-01

    Full Text Available OBJETIVOS: A faringoamigdalite aguda é uma das doenças mais freqüentes na prática pediátrica, sendo o estreptococo beta-hemolítico do grupo A (EBHGA o agente etiológico bacteriano mais comum. O seu diagnóstico e tratamento adequados são importantes principalmente para a prevenção de seqüelas não-supurativas. Testes rápidos de detecção de antígenos do estreptococo do grupo A são uma ferramenta útil no diagnóstico das faringoamigdalites estreptocócicas, pela rapidez dos resultados, acurácia e baixo custo; no entanto, são pouco utilizados em nosso meio e pouco estudados em nosso país. O objetivo deste estudo foi avaliar a acurácia de um kit de teste rápido de detecção de antígeno do EBHGA comparado à cultura de suabe de orofaringe. MÉTODOS: Foram selecionadas crianças de 1 a 18 anos com diagnóstico clínico de faringoamigdalite aguda em serviços públicos de urgência e clínica privada de Belo Horizonte (MG, sendo excluídas as que haviam utilizado antibióticos até 30 dias antes da consulta. A amostra final incluiu 229 pacientes, que foram submetidos a coleta de dois suabes de orofaringe, um para o teste rápido para EBHGA e o outro enviado para cultura. RESULTADOS: Encontrou-se sensibilidade de 90,7%, especificidade de 89,1%, valor preditivo positivo de 72,1%, valor preditivo negativo de 96,9% e razão de verossimilhança positiva de 9,0 para o teste rápido utilizado comparado à cultura. CONCLUSÃO: O teste rápido utilizado apresentou boa correlação com a cultura, sendo, portanto, de grande utilidade na prática clínica para detecção do EBHGA.OBJECTIVES: Acute pharyngitis is one of the most common diseases in pediatric practice, and the most common bacterial etiology is group A beta-hemolytic streptococcus (GABHS. Correct diagnosis and treatment are primarily of importance to the prevention of non-suppurative sequelae. Rapid tests for detecting the antigen of group A streptococcus are a useful tool for

  16. Quantitative computed tomography as a test of endurance for evaluation of bony plates; Utilizacao da tomografia computadorizada quantitativa como teste de resistencia para avaliacao de placas osseas

    Energy Technology Data Exchange (ETDEWEB)

    Melo Filho, E.V.; Costa, L.A.V.S.; Oliveira, D.C. [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil); Freitas, P.M.C. [Escola de Veterinaria - Universidade Federal de Minas Gerais - Belo Horizonte, MG (Brazil); Teixeira, M.W.; Costa, F.S. [Universidade Federal Rural de Pernambuco - Recife, PE (Brazil)

    2012-06-15

    Quantitative computed tomography was used to determine the radiodensity of bony plates. The CT scans provided information regarding radiodensity of bony plates and allowed to verify the uniformity of bone mineral density in their scope. The proposed methodology should be considered as another tool for determining the resistance of these biomaterials. (author)

  17. Prevalence of agglutinating antibodies to Sarcocystis neurona in skunks (Mephitis Mephitis), raccoons (Procyon lotor), and opossums (Didelphis Virginiana) from Connecticut.

    Science.gov (United States)

    Mitchell, Sheila M; Richardson, Dennis J; Cheadle, M Andy; Zajac, Anne M; Lindsay, David S

    2002-10-01

    Equine protozoal myeloencephalitis is the most important protozoan disease of horses in North America and is usually caused by Sarcocystis neurona. Natural cases of encephalitis caused by S. neurona have been reported in skunks (Mephitis mephitis) and raccoons (Procyon lotor). Opossums (Didelphis spp.) are the only known definitive host. Sera from 24 striped skunks, 12 raccoons, and 7 opossums (D. virginiana) from Connecticut were examined for agglutinating antibodies to S. neurona using the S. neurona agglutination test (SAT) employing formalin-fixed merozoites as antigen. The SAT was validated for skunk sera using pre- and postinfection serum samples from 2 experimentally infected skunks. Of the 24 (46%) skunks 11 were positive, and all 12 raccoons were positive for S. neurona antibodies. None of the 7 opossums was positive for antibodies to S. neurona. These results suggest that exposure to sporocysts of S. neurona by intermediate hosts is high in Connecticut. The absence of antibodies in opossums collected from the same areas is most likely because of the absence of systemic infection in the definitive host.

  18. The effects of oxotremorine, epibatidine, atropine, mecamylamine and naloxone in the tail-flick, hot-plate, and formalin tests in the naked mole-rat (Heterocephalus glaber)

    DEFF Research Database (Denmark)

    Dulu, Thomas D; Kanui, Titus I; Towett, Philemon K

    2014-01-01

    -plate, and the formalin tests. The effects of co-administration of the muscarinic receptor antagonist atropine, the nicotinic receptor antagonist mecamylamine, and the opioid receptor antagonist naloxone were also investigated. Oxotremorine and epibatidine induced a significant, dose-dependent antinociceptive effect...... in the tail-flick, hot-plate, and formalin tests, respectively. The effects of oxotremorine and epibatidine were blocked by atropine and mecamylamine, respectively. In all three nociceptive tests, naloxone in combination with oxotremorine or epibatidine enhanced the antinociceptive effects of the drugs....... The present study demonstrated that stimulation of muscarinic and nicotinic receptors produces antinociceptive effects in the naked-mole rat. The reversal effect of atropine and mecamylamine suggests that this effect is mediated by cholinergic receptors. As naloxone increases the antinociceptive effects...

  19. Approach to Deep Plate Loading Test of Self-reaction Force Method%自反力法深层平板载荷试验探讨

    Institute of Scientific and Technical Information of China (English)

    王晓伟; 林泽耿; 刘炳凯

    2014-01-01

    This article took a project of Guangdong as an example, introduced the technique of deep plate loading test of self-reaction force method in detail,and compared with the traditional method of deep plate loading test,and finally,problems that need to pay attention to deep plate loading test of self-reaction force method were put forward from the processing technology at the bottom of testing hole,keeping test hole straight,keeping stability of hole wall,and so on.%以广东某工程为例,详细介绍采用自反力法进行深层平板载荷试验测试技术,并将该方法与传统法深层平板载荷试验进行比较,最后从测试孔的孔底处理技术、测孔保直、孔壁稳定等方面提出采用自反力法进行深层平板载荷试验需要注意的问题。

  20. Identification and characterization of a putative agglutination/immobilization antigen on the surface of Cryptocaryon irritans.

    Science.gov (United States)

    Hatanaka, A; Umeda, N; Yamashita, S; Hirazawa, N

    2007-08-01

    The ciliated protozoan Cryptocaryon irritans, a parasite of seawater fishes, was found to express an antigen that elicits antibodies in rabbits and tiger puffer (Takifugu ruburipes). Serum from rabbits and fish immunized with theronts had agglutination/immobilization activity against theronts in vitro; fish serum antibody levels (measured by enzyme-linked immunosorbent assays: ELISA) correlated with this activity. Anti-theront antibody levels in fish were significantly higher in the immunized group as compared with control fish at 2 weeks after booster immunization (injection of bovine serum albumin; Student's t-test, Pagglutination/immobilization antigen. Indirect immunofluorescence staining of theronts suggested that this 32 kDa antigen was expressed on the surface of cilia. The full-length 32 kDa antigen cDNA contained 1147 basepairs, encoding a 328-amino acid protein including hydrophobic N- and C-termini. As with Tetrahymena and Paramecium spp., TAA and TAG appear to be used as glutamine codons in the 32 kDa antigen gene.

  1. Counterimmunoelectrophoresis and latex particle agglutination in the etiologic diagnosis of presumed bacterial pneumonia in pediatric patients.

    Science.gov (United States)

    Rusconi, F; Rancilio, L; Assael, B M; Bonora, G; Cerri, M; Pietrogrande, M C; Razon, S; Serafini, L; Torti, G; Vaggi, D

    1988-11-01

    A commercial latex agglutination (LA) kit (Wellcogen, Wellcome Diagnostics) used to detect bacterial polysaccharide antigens (Haemophilus influenzae type b and Streptococcus pneumoniae) was compared with a modified counterimmunoelectrophoresis technique and blood culture for etiologic diagnosis of presumptive bacterial pneumonia requiring hospitalization in 60 infants and children. Serum, urine and either sputum or nasopharyngeal secretions were collected during the first 5 days of therapy for antigen detection. Blood culture was positive in 6 of 52 (11.5%) of cases. Antigens were detected by counterimmunoelectrophoresis and/or LA in 13 of 60 (21.7%) serum samples, 2 of 16 (12.5%) unconcentrated urine samples, 19 of 42 (45.2%) urine samples concentrated 25-fold and 21 of 45 (46.7%) sputum or nasopharyngeal secretions. Antibiotic treatment for 5 days did not affect the antigen detection rate. Counter-immunoelectrophoresis was more sensitive than LA in serum and urine but not in sputum. However, because false positive reactions were frequently obtained with LA on nasopharyngeal secretions of an age-matched control group, this test appears unreliable.

  2. Control of sperm concentration is necessary for standardization of sperm cryopreservation in aquatic species: evidence from sperm agglutination in oysters.

    Science.gov (United States)

    Dong, Qiaoxiang; Huang, Changjiang; Tiersch, Terrence R

    2007-02-01

    A lack of standardization in sperm cryopreservation of aquatic organisms is one of the main reasons for inconsistency observed among various studies. In particular, there have been few attempts to standardize sperm concentration during procedural optimization. This study was intended to call attention to sperm concentration standardization through research of sperm agglutination in Pacific oysters Crassostrea gigas. Sperm agglutination after thawing is a relatively frequent phenomenon observed for various aquatic species, especially when sub-optimal cryopreservation protocols are used; however, no systematic attempts have been made to explain this phenomenon. The present study evaluated various factors affecting sperm agglutination of thawed samples from diploid and tetraploid Pacific oysters, and is the first detailed report addressing the sperm agglutination phenomenon of thawed samples from any aquatic organism. Agglutination of oyster sperm was classified into six levels with a scale ranging from 0 (homogenous suspension) to 5 (well-developed "noodles"). It was found that agglutination in thawed samples was mainly due to the lack of sufficient cryoprotectant for a specific sperm concentration. Interestingly, high levels of agglutination did not necessarily lead to low fertilization. On the contrary, some sperm cells appeared to gain protection from the formation of peripheral agglutination within 0.5-ml French straws. The exact mechanism of sperm agglutination remains unclear. However, morphological examination of cross sections of the noodles (agglutination level 5) indicated at least two forms of agglutination (formed with and without cryoprotectant) which could be used as a tool to understand the cryopreservation process within the micro-environment of the straw. Furthermore, the fact that the level of sperm agglutination was directly determined by sperm concentration, in addition to the type of cryoprotectant, cryoprotectant concentration, and cooling and

  3. Dodecamer is required for agglutination of Litopenaeus vannamei hemocyanin with bacterial cells and red blood cells.

    Science.gov (United States)

    Pan, Jian-yi; Zhang, Yue-ling; Wang, San-ying; Peng, Xuan-xian

    2008-01-01

    Hemocyanins are multi-functional proteins, although they are well known to be respiratory proteins of invertebrate to date. In the present study, the agglutination ability of two oligomers of hemocyanin, hexamer and dodecamer, with pathogenic bacteria and red blood cells (RBCs) is investigated in pacific white shrimp, Litopenaeus vannamei. Hexameric hemocyanin exhibits an extremely high stability even in the absence of Ca(2+) and in alkaline pH. Dodecamer (di-hexamer) is easily dissociated into hexamers in unphysiological conditions. Hexamer and dodecamer are interchanged reciprocally with environmental conditions. Both oligomers can bind to bacteria and RBCs, but agglutination is observed only using dodecamer but not using hexamer in agglutination assay. However, the agglutination is detected when hexamer is utilized in the presence of antiserum against hemocyanin. These results indicate that dodecamer of hemocyanin is required for agglutination with bacteria and RBCs. It can be logically inferred that there is only one carbohydrate-binding site to bacterial cells and RBCs in the hexamer, while at least two sites in the dodecamer. Our finding has provided new insights into structural-functional relationship of hemocyanin.

  4. Rheologic characterization of vegetal lectins by dissociation of induced erythrocyte agglutinates.

    Science.gov (United States)

    Rasia, R J; Valverde, J R; Gentils, M; Cauchois, C; Stoltz, J F

    1997-01-01

    Energy evolved from hemagglutination reaction or spent in dissociating erythrocyte agglutinates has been proved to be an excellent parameter for analyzing cell-cell interactions mediated by bridging molecules such as antibodies or lectins. We developed a new rheo-optical method to estimate the energy of dissociation of red blood cell agglutinates. In a Couette shear field agglutinates can be dissociated until a suspension of monodispersed cells is obtained. Intensity of light backscattered by suspended agglutinates increases during their mechanical dissociation. Variation of backscattered light intensity correlates with the energy spent in the process. The adhesive energy of erythrocyte agglutination induced by lectins has been estimated by applying this method. Two specific lectins (Dolichus Biflorus agglutinin and Ulex Europaeus agglutinin) and a new lectin obtained from Amarantus Cruentus seeds which specificity is unknown were studied. Results obtained in this work for Dolichus Biflorus lectin are comparable with values published by other authors. An asymptotic decrease of adhesive energy was observed when the mechanical dissociation was applied several times on the same sample. Our results suggest that the cell detachment is accompanied by the extraction of membrane receptors. This finding is consistent with results obtained by other authors.

  5. The Preliminary Analysis of Agglutinating Activity of Seven Kinds of Genuine Medicinal Materials in Gansu%7种甘肃道地药材凝血活性初步分析

    Institute of Scientific and Technical Information of China (English)

    袁毅君; 赵丽; 焦成瑾; 陈荃

    2016-01-01

    Objective: To test the agglutinating activity about seven kinds of Genuine Medicinal Materials in Gansu react with the red blood cells of chicken and rabbit respectively. Methods: Selected seven medicinal herbs were ex⁃tracted with PBS, then extracts agglutinating activity were detected with the red blood cells from rabbit and chicken respectively, determined the lowest titer of the herbs. Results: The agglutination activities of the lectins varied in the seven herbs extracts, and the higher agglutinating activity of the herb lectins in the two different blood cells are fol⁃lows: the titer of extracts from Radix Saposhnikoviae with the rabbit red blood cells is 23; but the titer of extracts from Radix et Rhizoma Rhel with chicken red blood cells is 25. Conclusion: Seven kinds of Chinese herbs present better agglutinating activity, the same Chinese herbs has different agglutinating activity with different erythrocyte samples.%目的:检测7种甘肃道地药材对鸡、兔红细胞的凝集活性。方法:将7种药材分别用PBS (磷酸缓冲液)浸提,浸提液分别对兔和鸡红细胞进行凝集活性测定,以检测7种药材的最低效价。结果:7种中药浸提液对红细胞表现不同的凝集活性,具有较高凝集活性的为防风对兔红细胞效价23;大黄对鸡红细胞效价25.结论:7种中药均有不同的凝集活性,同种中药对不同红细胞的凝集活性不同。

  6. 对虾血蓝蛋白多聚结构与凝集活性%Differences in the agglutination activity of two oligomers of hemocyanin from Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    潘建义; 吴海港; 王浩波

    2011-01-01

    目的 分析凡纳对虾血蓝蛋白六聚体和十二聚体两种结构形式的凝集活性差异. 方法 制备凡纳对虾血清,采用阴离子交换层析法分离血蓝蛋白,SDS-PAGE、Native-PAGE和质谱分析其多聚结构,玻片法红细胞凝集试验测定血蓝蛋白的凝集作用. 结果 离子交换层析得到2个明显的蛋白质峰,经电泳和质谱分析,二者分别为血蓝蛋白的六聚体和十二聚体,且十二聚体可凝集人红细胞,而六聚体无凝集活性. 结论 凡纳对虾血蓝蛋白主要以十二聚体形式存在,且具有凝集活性.%Objective To investigate differences in the agglutination activity of two oligomers, a hexamer and a dodecamer, of hemocyanin from Litopenaeus vannamei.Methods Prepared shrimp sera were isolated by anion exchange chromatography, and obtained oligomers were identified by combined SDS-PAGE, Native-PAGE, and mass spectrometry analysis.A red blood cell agglutination test was used to analyze differences in the agglutination ability of the two oligomers.Results Two distinct protein peaks were obtained in ion exchange chromatography, and these peaks were identified as a hexamer and dodecamer of hemocyanin.The shrimp sera and dodecamer caused human red blood cells to agglutinate while the hexamer was not found to have agglutination activity.Conclusion The dodecamer of hemocyanin from L.vannamei is active under physiological conditions and has agglutination activity.

  7. Creep-fatigue evaluation method for weld joint of Mod.9Cr-1Mo steel Part II: Plate bending test and proposal of a simplified evaluation method

    Energy Technology Data Exchange (ETDEWEB)

    Ando, Masanori, E-mail: ando.masanori@jaea.go.jp; Takaya, Shigeru, E-mail: takaya.shigeru@jaea.go.jp

    2016-12-15

    Highlights: • Creep-fatigue evaluation method for weld joint of Mod.9Cr-1Mo steel is proposed. • A simplified evaluation method is also proposed for the codification. • Both proposed evaluation method was validated by the plate bending test. • For codification, the local stress and strain behavior was analyzed. - Abstract: In the present study, to develop an evaluation procedure and design rules for Mod.9Cr-1Mo steel weld joints, a method for evaluating the creep-fatigue life of Mod.9Cr-1Mo steel weld joints was proposed based on finite element analysis (FEA) and a series of cyclic plate bending tests of longitudinal and horizontal seamed plates. The strain concentration and redistribution behaviors were evaluated and the failure cycles were estimated using FEA by considering the test conditions and metallurgical discontinuities in the weld joints. Inelastic FEA models consisting of the base metal, heat-affected zone and weld metal were employed to estimate the elastic follow-up behavior caused by the metallurgical discontinuities. The elastic follow-up factors determined by comparing the elastic and inelastic FEA results were determined to be less than 1.5. Based on the estimated elastic follow-up factors obtained via inelastic FEA, a simplified technique using elastic FEA was proposed for evaluating the creep-fatigue life in Mod.9Cr-1Mo steel weld joints. The creep-fatigue life obtained using the plate bending test was compared to those estimated from the results of inelastic FEA and by a simplified evaluation method.

  8. An unusual presentation of brucellosis, involving multiple organ systems, with low agglutinating titers: a case report

    Directory of Open Access Journals (Sweden)

    Khorvash Farzin

    2007-07-01

    Full Text Available Abstract Background Brucellosis is a multi-system disease that may present with a broad spectrum of clinical manifestations. While hepatic involvement in brucellosis is not rare, it may rarely involve the kidney or display with cardiac manifestations. Central nervous system involvement in brucellosis sometimes can cause demyelinating syndromes. Here we present a case of brucella hepatitis, myocarditis, acute disseminated encephalomyelitis, and renal failure. Case presentation A 26-year-old man presented with fever, ataxia, and dysarthria. He was a shepherd and gave a history of low grade fever, chilly sensation, cold sweating, loss of appetite, arthralgia and 10 Kg weight loss during the previous 3 months. He had a body temperature of 39°C at the time of admission. On laboratory tests he had elevated level of liver enzymes, blood urea nitrogen, Creatinine, Creatine phosphokinase (MB, and moderate proteinuria. He also had abnormal echocardiography and brain MRI. Enzyme-linked immunosorbent assay for IgG and IgM was negative. Standard tube agglutination test (STAT and 2-mercaptoethanol (2-ME titers were 1:80 and 1:40 respectively. Finally he was diagnosed with brucellosis by positive blood culture and the polymerase chain reaction for Brucella mellitensis. Conclusion In endemic areas clinicians should consider brucellosis in any unusual presentation involving multiple organ systems, even if serology is inconclusive. In endemic areas low STAT and 2-ME titers should be considered as an indication of brucellosis and in these cases additional testing is recommended to rule out brucellosis.

  9. A simple system for in-droplet incubation and quantification of agglutination assays

    KAUST Repository

    Castro, David

    2013-10-28

    This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in micro-channels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (~2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.

  10. Discovery of agglutinated foraminifers from the Longzhaogou Group in eastern Heilongjiang Province

    Institute of Scientific and Technical Information of China (English)

    LI Gang; YU Shanmao

    2004-01-01

    The low diversity agglutinated foraminifers are recovered from the Qihulin Formation of the Longzhaogou Group in eastern Heilongjiang, China. The foraminiferal fauna consists of 9 species of 5 genera. The common members are Cribrostomoides nonioninoides (Reuss), Haplophragmoides concavus (Chapman), H. gigas minor Nauss. Although the diagnostic zonal taxa are absent in the agglutinated fauna, according to the global stratigraphic distribution of the above-mentioned species, and the associated Pseudohaploceras ammonite fauna, the foraminiferal fauna may be of a Barremian-Aptian (Early Cretaceous) age.

  11. Seasonal variation in agglutination of Plasmodium falciparum-infected erythrocytes

    DEFF Research Database (Denmark)

    Giha, H A; Theander, T G; Staalsø, T;

    1998-01-01

    place in the absence of disease, presumably as a consequence of subclinical infection. This is the first demonstration of marked seasonal fluctuations in the capacity of individuals' sera to agglutinate parasitized red blood cells. Possible explanations for this effect include a decrease in the levels...... malaria infection samples taken from five of the cohort members. Our data show that the capacity of donor plasma samples to agglutinate parasitized cells depended largely on the time of sampling relative to the transmission season, at least within this epidemiologic setting. Thus, although less than half...

  12. Diagnostic Efficacy of Modified Coagglutination Test in the Diagnosis of Human Brucellosis

    Directory of Open Access Journals (Sweden)

    Mohite S.T

    2013-07-01

    Full Text Available Background: Laboratory help is must for thediagnosis of human brucellosis due to proteanclinical manifestations. As culture is hazardous,time consuming and less sensitive, serologicaltests are preferred for the diagnosis. Aggluti-nation tests like Rose Bengal PlateTest (RBPT, Serum Agglutination tests (SAT,2-Mercaptoethanol test (2-ME that are com-monly employed for the diagnosis either lacksensitivity or specificity. Coombs test andBrucellacapt though are sensitive and specific,workout costly. Therefore, modifiedcoagglutination test was developed and its di-agnostic efficacy was evaluated. Aims and Ob-jectives: To develop modified coagglutinationtest for the diagnosis of human brucellosis andcompare it with Coombs test. Materials andMethods: Serum samples collected from 191brucellosis patients and 100 controls were sub-jected to 2-ME, Coombs test and modifiedcoagglutination test (MCOAG. Blood culturewas performed by Castaneda’s method in all thepatients. Results: Significant difference in thepositivity rate was seen between MCOAG and2-ME. The results of MCOAG were compa-rable with Coombs test. Conclusions: Modi-fied coagglutination test is a better option toCoombs test for the serodiagnosis of brucel-losis in resource constrained countries as it issensitive, specific and cost effective.

  13. Outbreak of uncommon O4 non-agglutinating Salmonella typhimurium linked to minced pork, Saxony-Anhalt, Germany, January to April 2013.

    Directory of Open Access Journals (Sweden)

    Katja Alt

    Full Text Available In January 2013, the National Reference Centre for Salmonella (NRC detected a salmonellosis cluster in Saxony-Anhalt, Germany, caused by uncommon O4 non-agglutinating, monophasic Salmonella (S. Typhimurium DT193. Circulating predominant monophasic S. Typhimurium DT193 clones typically display resistance phenotype ASSuT. We investigated common exposures to control the outbreak, and conducted microbiological investigations to assess the strains' phenotype.We conducted a case-control study defining cases as persons living or working in Saxony-Anhalt diagnosed with the O4 non-agglutinating strain between January and March 2013. We selected two controls contemporarily reported with norovirus infection, frequency-matched on residence and age group, per case. We interviewed regarding food consumption, especially pork and its place of purchase. We calculated odds ratios (ORs with 95% confidence intervals (95% CI using logistic regression. The NRC investigated human and food isolates by PCR, SDS-PAGE, MLST, PFGE, MLVA and susceptibility testing.Altogether, 68 O4 non-agglutinating human isolates were confirmed between January and April 2013. Of those, 61 were assigned to the outbreak (median age 57 years, 44% female; 83% cases ≥ 60 years were hospitalized. Eating raw minced pork from butcheries within 3 days was associated with disease (31 cases, 28 controls; OR adjusted for sex: 3.6; 95% CI: 1.0-13. Phage type DT193 and MLST ST34 were assigned, and isolates' lipopolysaccharide (LPS matched control strains. Isolates linked to Saxony-Anhalt exhibited PFGE type 5. ASSuT- and ACSSuT phenotype proportions were 34 and 39% respectively; 54% were resistant to chloramphenicol. Three pork isolates matched the outbreak strain.Raw minced pork was the most likely infection vehicle in this first reported outbreak caused by O4 non-agglutinating, mostly chloramphenicol-resistant S. Typhimurium DT193. High hospitalization proportions demand awareness on the risk of

  14. A soro-aglutinação das Leishmanias Agglutination of Leishmanias

    Directory of Open Access Journals (Sweden)

    A. M. da Cunha

    1942-01-01

    Full Text Available The first agglutination experiments (Tables 1 and 2 showed that the serum obtained with any one strain of Leishmania, agglutinates all the others even of another species. This finding reveals the existence of a common antigen. However as the titre of agglutination did not permit a sharp differentiation of species we tried the adsorption method. The first adsorption tests made demonstrated differences in antigenic constitution between a strain of. L. donovani on one hand and strains of L. tropica or L. brasiliensis on the other. Further experiments in which L. chagasi was tested against the other species revealed that the former was antigenically different from the others. These tests were performed by adsorbing an anti-chagasi serum with organisms belonging to the other species or, conversely, adsorbing with L. chagasi sera prepared against the other species (See Tables 9 to 24. On the other hand, the adsorption of a serum prepared against one strain of l. chagasi by another of the same species showed that they had identifical antigenie constitution. These findings suggested the possibility of separating different species of Leishmania by this method. However, tests to separate the other species from one to another gave inconclusive results. (See Tables 27 to 35. It was soon observed that all the strains of L. chagasi were of recent isolation while all the others had been maintained in artificial culture media for a long time. We were led to believe that this condition was responsible for the differences in behaviour encountered. Accordingly, recently isolated strains of L. brasiliensis and L. donovani were tested and shown to be antigenically similar to strains of L. chagasi also recently isolated. The conclusion may be drawn that all strains have the same antigenic constitution when freshly isolated. It has been noted that when a serum which has been prepared against a freshly isolated is adsorbed with an old strain, the amount of agglutinins

  15. Tests of new physics from precise measurements of the Casimir pressure between two gold-coated plates

    CERN Document Server

    Decca, R S; Klimchitskaya, G L; Krause, D E; López, D; Mostepanenko, V M

    2007-01-01

    A micromechanical torsion oscillator has been used to strengthen the limits on new Yukawa forces by determining the Casimir pressure between two gold-coated plates. By significantly reducing the random errors and obtaining the electronic parameters of the gold coatings, we were able to conclusively exclude the predictions of large thermal effects below 1 $\\mu$m and strengthen the constraints on Yukawa corrections to Newtonian gravity in the interaction range from 29.5 nm to 86 nm.

  16. Real time observation and automated measurement of red blood cells agglutination inside a passive microfluidic biochip containing embedded reagents.

    Science.gov (United States)

    Huet, Maxime; Cubizolles, Myriam; Buhot, Arnaud

    2016-09-20

    The process of agglutination is commonly used for the detection of biomarkers like proteins or viruses. The multiple bindings between micrometer sized particles, either latex beads or red blood cells (RBCs), create aggregates that are easily detectable and give qualitative information about the presence of the biomarkers. In most cases, the detection is made by simple naked-eye observation of agglutinates without any access to the kinetics of agglutination. In this study, we address the development of a real-time time observation of RBCs agglutination. Using ABO blood typing as a proof-of-concept, we developed i) an integrated biological protocol suitable for further use as point-of-care (POC) analysis and ii) two dedicated image processing algorithms for the real-time and quantitative measurement of agglutination. Anti-A or anti-B typing reagents were dried inside the microchannel of a passive microfluidic chip designed to enhance capillary flow. A blood drop deposit at the tip of the biochip established a simple biological protocol. In situ agglutination of autologous RBCs was achieved by means of embedded reagents and real time agglutination process was monitored by video recording. Using a training set of 24 experiments, two real-time indicators based on correlation and variance of gray levels were optimized and then further confirmed on a validation set. 100% correct discrimination between positive and negative agglutinations was performed within less than 2min by measuring real-time evolution of both correlation and variance indicators.

  17. Gelatin particle indirect agglutination and enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis using Strongyloides venezuelensis antigen.

    Science.gov (United States)

    Huaman, Maria Cecilia; Sato, Yoshiya; Aguilar, Jose Luis; Terashima, Angelica; Guerra, Humberto; Gotuzzo, Eduardo; Kanbara, Hiroji

    2003-01-01

    Routine microscopical examination of stool specimens for diagnosis of strongyloidiasis is insensitive and serological methods using Strongyloides stercoralis antigen are at present not available for field studies. We evaluated 2 techniques, enzyme-linked immunosorbent assay (ELISA) and gelatin particle indirect agglutination (GPIA), using an antigen obtained from the rodent parasite, S. venezuelensis. Fifty-four Peruvian patients with different clinical forms of strongyloidiasis were studied: 12 asymptomatic, 31 symptomatic, and 11 hyperinfection cases. Our results demonstrate that both ELISA and GPIA using S. venezuelensis antigen are useful for diagnosis of strongyloidiasis, with sensitivities of 74.1% and 98.2%, respectively and a specificity of 100% for both techniques. We found that GPIA is a highly sensitive test for patients with suspected chronic infection and/or hyperinfection. In the hyperinfection cases, significantly lower concentrations of specific immunoglobulin antibodies and eosinophils (P < 0.001) were found compared with the asymptomatic and symptomatic cases.

  18. Experimental tests of irradiation-anneal-reirradiation effects on mechanical properties of RPV plate and weld materials

    Energy Technology Data Exchange (ETDEWEB)

    Rosinski, S.T. [Electric Power Research Inst., Charlotte, NC (United States); Hawthorne, J.R. [Materials Engineering Associates, Inc., Lanham, MD (United States); Rochau, G.E. [Sandia National Labs., Albuquerque, NM (United States)

    1999-10-01

    The Charpy-V (C{sub v}) notch ductility and tensile properties of three reactor pressure vessel (RPV) steel materials were determined for the 288 C (550 F) irradiated (I), 288 C (550 F) irradiated + 454 C (850 F) - 168 h postirradiation annealed (IA), and 288 C (550 F) reirradiated (IAR) conditions. Total fluences of the I condition and the IAR condition were, respectively, 3.33 {times} 10{sup 19} n/cm{sup 2} and 4.18 {times} 10{sup 19} n/cm{sup 2}, E > 1 MeV. The irradiation portion of the IAR condition represents an incremental fluence increase of 1.05 {times} 10{sup 19} n/cm{sup 2}, E > 1 MeV, over the I-condition fluence. The annealing treatment produced full C{sub v} upper shelf recovery and full or nearly full recovery in the C{sub v} 41 J (30 ft-lb) transition temperature. The C{sub v} transition temperature increases produced by the reirradiation exposure were 22% to 43% of the increase produced by the first cycle irradiation exposure. A somewhat greater radiation embrittlement sensitivity and a somewhat greater reirradiation embrittlement sensitivity were exhibited by the low nickel content plate than the high nickel content plate. The IAR-condition properties of the surface vs. interior regions of the low nickel content plate are also compared.

  19. The Test and Study of RPC Capping Plate Bearing Capacity and Crack Resistance%RPC盖板承载力与抗裂性试验研究

    Institute of Scientific and Technical Information of China (English)

    黄旭毅; 吴伟

    2013-01-01

      以京沪高速铁路三标段曲阜 RPC 混凝土盖板厂为依托,介绍 WDW -10型微机监测高强道板抗弯承载力试验机的开发和设计情况,并对 RPC 盖板承载力与抗裂性进行试验研究。%  The paper briefs the development and the design of the type WDW – 10 microcomputer - moni-tored high strength side walk slab bending resistant bearing capacity tester by relying on Qufu RPC Cap -ping Plate Precast Factory for the Bid 3 section of the Beijing - Shanghai High Speed Railway .Both test and study of RPC capping plate bearing capacity and crack resistance have been performed .

  20. Single agglutinates: A comparative study of compositions of agglutinitic glass, whole-grain, bulk soil, and FMR

    Science.gov (United States)

    Basu, A.; Robinson, R.; Mckay, D. S.; Blanchard, D. P.; Morris, R. V.; Wentworth, Susan J.

    1994-01-01

    Previous workers on single agglutinates have variously interpreted the composition of agglutinitic glass to represent impact melts of (1) bulk soil, (2) mixed components in finer sizes, and (3) microtargets. Separately, Papike has argued in favor of fusion of the finest fraction of bulk soils. Thirty-four single agglutinates were hand-picked from the mature Apollo 16 soil 61181 (I(sub s)/FeO = 82) and the FMR and chemical composition (INAA for Fe, Sc, Sm, Co, Ni, and Cr) of each agglutinate particle were measured. Thirteen of these single agglutinates were selected for electron beam microanalysis and imaging. Less than 1 micron spots were analyzed (for Na, Mg, Al, Si, P, S, K, Ca, Ti, Cr, Mn, Fe, Ni, and Ba) on pure glassy areas (approximately ten in each particle) selected on the basis of optical and BSE images (avoiding all clasts and inclusions) with an electron microprobe to obtain average glass compositions of each single agglutinate.

  1. 9 CFR 93.432 - Cattle from the Republic of Ireland.

    Science.gov (United States)

    2010-01-01

    ... days of the date of export; (i) Plate or Tube agglutination test conducted in dilutions to detect... Plate or Tube agglutination test, negative to the brucellosis card test and negative to the CF test as...) Cattle showing a serological titer more than 60 IU to the Plate or Tube agglutination test, or a...

  2. Reading Development in Agglutinative Languages: Evidence from Beginning, Intermediate, and Adult Basque Readers

    Science.gov (United States)

    Acha, Joana; Laka, Itziar; Perea, Manuel

    2010-01-01

    Do typological properties of language, such as agglutination (i.e., the morphological process of adding affixes to the lexeme of a word), have an impact on the development of visual word recognition? To answer this question, we carried out an experiment in which beginning, intermediate, and adult Basque readers (n = 32 each, average age = 7, 11,…

  3. Agglutination of pYV+ Yersinia enterocolitica strains by agglutinin from Mangifera indica.

    OpenAIRE

    Wauters, Georges; Charlier, Jacqueline; Janssens, Michèle

    1995-01-01

    Agglutination of 271 strains of Yersinia enterocolitica and related species grown at 37 degrees C by a 0.01% dilution of the agglutinin from Mangifera indica was correlated with the presence of the virulence plasmid. The study of YadA mutants suggested that the YadA protein is the target of the plant agglutinin.

  4. Sorption and agglutination phenomenon of nanofluids on a plain heating surface during pool boiling

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Zhen-hua; Liao, Liang [School of Mechanical and Power Engineering, Shanghai Jiaotong University, 200030 Shanghai (China)

    2008-05-15

    The pool nucleate boiling heat transfer experiments of water (H{sub 2}O) based and alcohol (C{sub 2}H{sub 5}OH) based nanofluids and nanoparticles-suspensions on the plain heated copper surface were carried out. The study was focused on the sorption and agglutination phenomenon of nanofluids on a heated surface. The nanofluids consisted of the base liquid, the nanoparticles and the surfactant. The nanoparticles-suspensions consisted of the base liquid and nanoparticles. The both liquids of water and alcohol and both nanoparticles of CuO and SiO{sub 2} were used. The surfactant was sodium dodecyl benzene sulphate (SDBS). The experimental results show that for nanofluids, the agglutination phenomenon occurred on the heated surface when the wall temperature was over 112{sup o}C and steady nucleated boiling experiment could not be carried out. The reason was that an unsteady porous agglutination layer was formed on the heated surface. However, for nanoparticles-suspensions, no agglutination phenomenon occurred on the heating surface and the steady boiling could be carried out in the whole nucleate boiling region. For the both of alcohol based nanofluids and nano-suspensions, no agglutination phenomenon occurred on the heating surface and steady nucleate boiling experiment could be carried out in the whole nucleate boiling region whose wall temperature did not exceed 112{sup o}C. The boiling heat transfer characteristics of the nanofluids and nanoparticles-suspensions are somewhat poor compared with that of the base fluids, since the decrease of the active nucleate cavities on the heating surface with a very thin nanoparticles sorption layer. The very thin nanoparticles sorption layer also caused a decrease in the solid-liquid contact angle on the heating surface which leaded to an increase of the critical heat flux (CHF). (author)

  5. A critical role for the regulation of Syk from agglutination to aggregation in human platelets.

    Science.gov (United States)

    Shih, Chun-Ho; Chiang, Tin-Bin; Wang, Wen-Jeng

    2014-01-10

    Agglucetin, a tetrameric glycoprotein (GP) Ibα agonist from Formosan Agkistrodon acutus venom, has been characterized as an agglutination inducer in human washed platelets (WPs). In platelet-rich plasma (PRP), agglucetin dramatically elicits a biphasic response of agglutination and subsequent aggregation. For clarifying the intracellular signaling events from agglutination to aggregation in human platelets, we examined the essential signaling molecules involved through the detection of protein tyrosine phosphorylation (PTP). In WPs, an anti-GPIbα monoclonal antibody (mAb) AP1, but not a Src kinase inhibitor PP1, completely inhibited agglucetin-induced agglutination. However, PP1 but not AP1 had a potent suppression on platelet aggregation by a GPVI activator convulxin. The PTP analyses showed agglucetin alone can cause a weak pattern involving sequential phosphorylation of Lyn/Fyn, Syk, SLP-76 and phospholipase Cγ2 (PLCγ2). Furthermore, a Syk-selective kinase inhibitor, piceatannol, significantly suppressed the aggregating response in agglucetin-activated PRP. Analyzed by flow cytometry, the binding capacity of fluorophore-conjugated PAC-1, a mAb recognizing activated integrin αIIbβ3, was shown to increase in agglucetin-stimulated platelets. Again, piceatannol but not PP1 had a concentration-dependent suppression on agglucetin-induced αIIbβ3 exposure. Moreover, the formation of signalosome, including Syk, SLP-76, VAV, adhesion and degranulation promoting adapter protein (ADAP) and PLCγ2, are required for platelet aggregation in agglucetin/fibrinogen-activated platelets. In addition, GPIbα-ligation via agglucetin can substantially promote the interactions between αIIbβ3 and fibrinogen. Therefore, the signal pathway of Lyn/Fyn/Syk/SLP-76/ADAP/VAV/PLCγ2/PKC is sufficient to trigger platelet aggregation in agglucetin/fibrinogen-pretreated platelets. Importantly, Syk may function as a major regulator for the response from GPIbα-initiated agglutination to

  6. Plate Penetration Tests of the 2000 lb Bomb G. P. (Low Drag) Type EX-11, Mod 0

    Science.gov (United States)

    1952-04-07

    ly. lÄmt%,sfi?r’ noch in« t .-J prcviflti» impact*. ’ --r^^*g^<----y • - ^’ jfe*V_. T>c •’•I .1 Bi^.-„ Ijtitttli ’bnUusr.-Otirjr- V -fe...plate • fe~ Thickness at impact __ . # OBLIQUITY ~’£0*~ MIL UL1M: U0/h, Impact-dimensions [9"_X £ɛ - TENETKÄT1ÖN ~ * flaking

  7. 侵彻多层钢靶板过载测试技术%Overload Testing Technology of Penetrating Multi-Layer Steel Target Plate

    Institute of Scientific and Technical Information of China (English)

    张美云; 王敬斌; 王丹; 石庚辰

    2016-01-01

    针对在弹丸侵彻钢靶板过程中高过载带来的问题,研究了一种能够抗高冲击作用的弹丸侵彻多层钢靶板过载测试装置。该装置采用灌封、多层材料组合缓冲以及二次电源的措施,能保证测试装置抗高冲击作用和可靠工作。对弹丸侵彻多层钢靶板的过载特性进行了测试,结果表明所研究的测试装置能够可靠获取与记录弹丸穿过各层靶板时的加速度。%This paper presented a testing device with the ability of resisting high shock while penetrating multi-layer steel target plate to guarantee the high overload problem with plate process in the projectile penetrating steel target. Combined with potting technology, multi-layer shock absorb materials and secondary electrical power source,the device shows resistance to high shock and the ability of reliable working.Experiments on overload characteristic further proved that the device can collect and store the acceleration of penetrating multi-layer target plate with high reliability.

  8. An investigation of the typical corrosion parameters used to test polymer electrolyte fuel cell bipolar plate coatings, with titanium nitride coated stainless steel as a case study

    Science.gov (United States)

    Orsi, A.; Kongstein, O. E.; Hamilton, P. J.; Oedegaard, A.; Svenum, I. H.; Cooke, K.

    2015-07-01

    Stainless steel bipolar plates (BPP) for polymer electrolyte membrane fuel cells (PEMFCs) have good manufacturability, durability and low costs, but inadequate corrosion resistance and elevated interfacial contact resistance (ICR) in the fuel cell environment. Thin film coatings of titanium nitride (TiN) of 1 μm in thickness, were deposited by means of physical vapour deposition (PVD) process on to stainless steel (SS) 316L substrates and were evaluated, in a series of tests, for their level of corrosion protection and ICR. In the ex-situ corrosion tests, variables such as applied potential, experimental duration and pH of the sulphate electrolyte at 80 °C were altered. The ICR values were found to increase after exposure to greater applied potentials and electrolytes of a higher pH. In terms of experimental duration, the ICR increased most rapidly at the beginning of each experiment. It was also found that the oxidation of TiN was accelerated after exposure to electrolytes of a higher pH. When coated BPPs were incorporated into an accelerated fuel cell test, the degradation of the fuel cell cathode resembled the plates that were tested at the highest anodic potential (1.4 VSHE).

  9. Vehicle License Plate Recognition Syst

    Directory of Open Access Journals (Sweden)

    Meenakshi,R. B. Dubey

    2012-12-01

    Full Text Available The vehicle license plate recognition system has greater efficiency for vehicle monitoring in automatic zone access control. This Plate recognition system will avoid special tags, since all vehicles possess a unique registration number plate. A number of techniques have been used for car plate characters recognition. This system uses neural network character recognition and pattern matching of characters as two character recognition techniques. In this approach multilayer feed-forward back-propagation algorithm is used. The performance of the proposed algorithm has been tested on several car plates and provides very satisfactory results.

  10. Aptamers that bind to the hemagglutinin of the recent pandemic influenza virus H1N1 and efficiently inhibit agglutination.

    Science.gov (United States)

    Gopinath, Subash C B; Kumar, Penmetcha K R

    2013-11-01

    Influenza virus hemagglutinin (HA) mediates both receptor (glycan) binding and membrane fusion for cell entry and has been the basis for typing influenza A viruses. In this study we have selected RNA aptamers (D-12 and D-26) that specifically target the HA protein of the recent pandemic influenza virus pdmH1N1 (A/California/07/2009). Among the selected aptamers the D-26 aptamer showed higher affinity for the HA of pdmH1N1 and was able to distinguish HA derived from other sub-types of influenza A viruses. The affinity of the D-26 aptamer was further improved upon incorporation of 2'-fluoropyrimidines to a level of 67 fM. Furthermore, the high affinity D-12 and D-26 aptamers were tested for their ability to interfere with HA-glycan interactions using a chicken red blood cell (RBC) agglutination assay. At a concentration of 200 nM the D-26 aptamer completely abolished the agglutination of RBCs, whereas D-12 only did so at 400 nM. These studies suggest that the selected aptamer D-26 not only has a higher affinity and specificity for the HA of pdmH1N1 but also has a better ability to efficiently interfere with HA-glycan interactions compared with the D-12 aptamer. The D-26 aptamer warrants further study regarding its application in developing topical virucidal products against the pdmH1N1 virus and also in surveillance of the pdmH1N1 influenza virus.

  11. Combined albumin and bicarbonate induces head-to-head sperm agglutination which physically prevents equine sperm-oviduct binding.

    Science.gov (United States)

    Leemans, Bart; Gadella, Bart M; Stout, Tom A E; Sostaric, Edita; De Schauwer, Catharina; Nelis, Hilde; Hoogewijs, Maarten; Van Soom, Ann

    2016-04-01

    In many species, sperm binding to oviduct epithelium is believed to be an essential step in generating a highly fertile capacitated sperm population primed for fertilization. In several mammalian species, this interaction is based on carbohydrate-lectin recognition. D-galactose has previously been characterized as a key molecule that facilitates sperm-oviduct binding in the horse. We used oviduct explant and oviduct apical plasma membrane (APM) assays to investigate the effects of various carbohydrates; glycosaminoglycans; lectins; S-S reductants; and the capacitating factors albumin, Ca(2+) and HCO3(-) on sperm-oviduct binding in the horse. Carbohydrate-specific lectin staining indicated that N-acetylgalactosamine, N-acetylneuraminic acid (sialic acid) and D-mannose or D-glucose were the most abundant carbohydrates on equine oviduct epithelia, whereas D-galactose moieties were not detected. However, in a competitive binding assay, sperm-oviduct binding density was not influenced by any tested carbohydrates, glycosaminoglycans, lectins or D-penicillamine, nor did the glycosaminoglycans induce sperm tail-associated protein tyrosine phosphorylation. Furthermore, N-glycosidase F (PNGase) pretreatment of oviduct explants and APM did not alter sperm-oviduct binding density. By contrast, a combination of the sperm-capacitating factors albumin and HCO3(-) severely reduced (>10-fold) equine sperm-oviduct binding density by inducing rapid head-to-head agglutination, both of which events were independent of Ca(2+) and an elevated pH (7.9). Conversely, neither albumin and HCO3(-) nor any other capacitating factor could induce release of oviduct-bound sperm. In conclusion, a combination of albumin and HCO3(-) markedly induced sperm head-to-head agglutination which physically prevented stallion sperm to bind to oviduct epithelium.

  12. Interest of retro-anal levator plate myorrhaphy in selected cases of descending perineum syndrome with positive anti-sagging test

    Directory of Open Access Journals (Sweden)

    Beco Jacques

    2008-07-01

    Full Text Available Abstract Background Levator plate sagging (LPS, usually called descending perineum syndrome, is one of the main defects encountered in perineology. This defect is classically associated with colo-proctologic functional troubles (dyschesia and anal incontinence but can also induce perineodynia, gynaecological and lower urinary tract symptoms. Methods A retrospective case series of nine female patients (mean age: 44.3 underwent an isolated retro-anal levator plate myorrhaphy (RLPM to treat symptomatic LPS confirmed by rectal examination and/or Perineocaliper®. An anti-sagging test (support of the posterior perineum must significantly improve the symptoms that were resistant to conservative treatment. The effect of the procedure on the symptoms of the 3 axes of the perineum (urological, colo-proctologic and gynecological and on perineodynia was evaluated during a follow up consultation more than 9 months after surgery. The effect of RLPM on the position of the anal margin and on the levator plate angle was studied using rectal examination, Perineocaliper® and retro-anal ultrasound. Results Before surgery, anti-sagging tests were positive for dyschesia, urinary urgency and pain. After a mean follow-up of 16.1 months, RLPM resolved or improved 2/2 cases of stress urinary incontinence, 3/5 of urinary urgency, 3/4 of dysuria, 3/3 of anal incontinence, 7/8 of dyschesia, 3/4 of cystocele, 4/5 of rectocele, 5/8 of dyspareunia and 6/7 of perineodynia. Rectal examination showed a complete suppression of sagging in 4 patients and an improvement in the 5 others. The mean reduction of perineal descent was 1.08 cm (extremes: 0–1.5. Using retro-anal ultrasound of the levator plate, the mean reduction of sagging was 12.67 degrees (extremes: 1 – 21. Conclusion Anti-sagging tests can be used before surgery to simulate the effect of RLPM. This surgical procedure seems to improve stress urinary incontinence, frequency, nocturia, urgency, dysuria, anal

  13. Foraminiferans as food for Cephalaspideans (Gastropoda: Opisthobranchia), with notes on secondary tests around calcareous foraminiferans

    DEFF Research Database (Denmark)

    Cedhagen, Tomas

    1996-01-01

    species, Ammonia batavus and two agglutinating species, Ammoscalaria pseudospiralis and Ammotium cassis. The test (shell) material of the latter two species was sand grains (quartz). It was inferred that the gastropods avoid agglutinating foraminiferans as food. Many calcareous but not agglutinating...... purposes. It might, in species like Ammonia batavus, have become a kind of antipredatory device or mimicry. A predator might conceive such a species as an agglutinating species and neglect it. The secondary test is a delicate structure in most species and is easily destroyed by the rough sampling...

  14. License plate detection algorithm

    Science.gov (United States)

    Broitman, Michael; Klopovsky, Yuri; Silinskis, Normunds

    2013-12-01

    A novel algorithm for vehicle license plates localization is proposed. The algorithm is based on pixel intensity transition gradient analysis. Near to 2500 natural-scene gray-level vehicle images of different backgrounds and ambient illumination was tested. The best set of algorithm's parameters produces detection rate up to 0.94. Taking into account abnormal camera location during our tests and therefore geometrical distortion and troubles from trees this result could be considered as passable. Correlation between source data, such as license Plate dimensions and texture, cameras location and others, and parameters of algorithm were also defined.

  15. Mapping key interactions in the dimerization process of HBHA from Mycobacterium tuberculosis, insights into bacterial agglutination.

    Science.gov (United States)

    Esposito, Carla; Cantisani, Marco; D'Auria, Gabriella; Falcigno, Lucia; Pedone, Emilia; Galdiero, Stefania; Berisio, Rita

    2012-03-23

    HBHA is a cell-surface protein implicated in the dissemination of Mycobacterium tuberculosis (Mtb) from the site of primary infection. Its N-terminal coiled-coil region is also involved in bacterial agglutination. However, despite the importance of HBHA dimerization in agglutination, protein regions involved in dimerization are hitherto not known. Here, we mapped these regions by coupling peptide synthesis, biochemical and computational analyses, and identified structural determinants for HBHA monomer-monomer recognition. Importantly, we obtained the first molecule able to induce HBHA dimer disaggregation at 37°C, the typical growth temperature of Mtb. This result provides new opportunities towards the development of Mtb anti-aggregation molecules with therapeutic interest.

  16. Dimerisation and structural integrity of Heparin Binding Hemagglutinin A from Mycobacterium tuberculosis: implications for bacterial agglutination.

    Science.gov (United States)

    Esposito, Carla; Carullo, Paola; Pedone, Emilia; Graziano, Giuseppe; Del Vecchio, Pompea; Berisio, Rita

    2010-03-19

    Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is associated with its ability to induce bacterial agglutination, we investigated this process using experimental and modelling techniques. We here identified a short segment of HBHA whose presence is mandatory for the stability of folded conformation, whose denaturation is a reversible two-state process. Our data suggest that agglutination-driven cell-cell interactions do not occur via association of HBHA monomers, nor via association of HBHA dimers and open the scenario to a possible trans-dimerisation process.

  17. Lead isotopic studies of lunar soils - Their bearing on the time scale of agglutinate formation

    Science.gov (United States)

    Church, S. E.; Tilton, G. R.; Chen, J. H.

    1976-01-01

    Fines (smaller than 75 microns) and bulk soil were studied to analyze loss of volatile lead; losses of the order of 10% to 30% radiogenic lead during the production of agglutinates are assessed. Lead isotope data from fine-agglutinate pairs are analyzed for information on the time scale of micrometeorite bombardment, from the chords generated by the data in concordia diagrams. Resulting mean lead loss ages were compared to spallogenic gas exposure ages for all samples. Labile parentless radiogenic Pb residing preferentially on or in the fines is viewed as possibly responsible for aberrant lead loss ages. Bulk soils plot above the concordia curve (in a field of excess radiogenic Pb) for all samples with anomalous ages.

  18. [The mechanism of change in speed of agglutination of human erythrocytes under the influence of adrenaline].

    Science.gov (United States)

    Volodchenko, A I; Tsirkin, V I; Kostiaev, A A

    2014-01-01

    In the study of red blood cells of 80 men found that adrenaline (10(-10) - 10(-6) g/mL) and phenylephrine (10-(10) - 10(-6) g/mL) dose-dependently increase the speed of agglutination of red blood cells, according to the decrease in agglutination of the start time and ginipral (10(-10) - 10(-7) g/mL), on the contrary, decreases it. The effect of adrenaline and phenylephrine is blocked by nicergoline (10(-6) g/mL), increased obzidan (10(-6) g/mL) and does not change under the action ofyohimbine (10(-6) g/mL) and atenolol (10(-6) g/mL). These data indicate that the speed of agglutination increases with activation alpha1-adrenergic receptor (AR) and decreases in the activation of beta2-AR, while the activation of alpha2- and beta1-AR does not affect it. Trifluoperazine (10(-6) g/mL) as the calmodulin antagonist, barium chloride (10(-6) g/mL) as a blocked of Ca(2+)-dependent K(+)-channels and indomethacine (10(-6) g/mL) as an inhibitor of cyclooxygenase and phospholipase A2 inhibit the ability of adrenaline to increases the speed of agglutination of red blood cells. This suggests that the effect of adrenaline caused an increase in erythrocyte entry of Ca2+, activation of calmodulin, cyclooxygenase, phospholipase A2 and the release of K+ from red blood cell through the Ca(2+)-dependent K+ channels, which is regarded as a manifestation of eryptosis. Indirectly, this means that more efficient activation of alpha1-AR and beta2-AR, respectively, increases or, conversely, decreases the rate of eryptosis.

  19. Applying the digital-image-correlation technique to measure the deformation of an old building’s column retrofitted with steel plate in an in situ pushover test

    Indian Academy of Sciences (India)

    Shih-Heng Tung; Ming-Hsiang Shih; Wen-Pei Sung

    2014-06-01

    An in situ pushover test is carried out on an old building of Guan-Miao elementary school in south Taiwan. Columns of this building are seismically retrofitted with steel plate. The DIC (digital-image-correlation) technique is used to measure the deformation of the retrofitted column. The result shows that the DIC technique can be successfully applied to measure the relative displacement of the column. Additionally, thismethod leads to the measurement of relative displacements formany points on the column simultaneously. Hence, the column deformation curve, rotation and curvature can be determined using interpolation method. The resulting curvaturediagram reveals that the phenomenon of plastic hinge occurs at about 2% storey drift ratio, and that the DIC technique can be applied to measure column deformation in a full scale in situ test.

  20. Multiregional evaluation of the SimPlate heterotrophic plate count method compared to the standard plate count agar pour plate method in water.

    Science.gov (United States)

    Jackson, R W; Osborne, K; Barnes, G; Jolliff, C; Zamani, D; Roll, B; Stillings, A; Herzog, D; Cannon, S; Loveland, S

    2000-01-01

    A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35 degrees C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0. 95; y = 0.99X + 0.06).

  1. Multiregional Evaluation of the SimPlate Heterotrophic Plate Count Method Compared to the Standard Plate Count Agar Pour Plate Method in Water

    OpenAIRE

    Jackson, R. Wayne; Osborne, Karen; Barnes, Gary; Jolliff, Carol; Zamani, Dianna; Roll, Bruce; Stillings, Amy; Herzog, David; Cannon, Shelly; Loveland, Scott

    2000-01-01

    A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35°C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0.95; y = 0.99X + 0.06).

  2. D-penicillamine prevents ram sperm agglutination by reducing the disulphide bonds of a copper-binding sperm protein.

    Science.gov (United States)

    Leahy, T; Rickard, J P; Aitken, R J; de Graaf, S P

    2016-05-01

    Head-to-head agglutination of ram spermatozoa is induced by dilution in the Tyrode's capacitation medium with albumin, lactate and pyruvate (TALP) and ameliorated by the addition of the thiol d-penicillamine (PEN). To better understand the association and disassociation of ram spermatozoa, we investigated the mechanism of action of PEN in perturbing sperm agglutination. PEN acts as a chelator of heavy metals, an antioxidant and a reducing agent. Chelation is not the main mechanism of action, as the broad-spectrum chelator ethylenediaminetetraacetic acid and the copper-specific chelator bathocuproinedisulfonic acid were inferior anti-agglutination agents compared with PEN. Oxidative stress is also an unlikely mechanism of sperm association, as PEN was significantly more effective in ameliorating agglutination than the antioxidants superoxide dismutase, ascorbic acid, α-tocopherol and catalase. Only the reducing agents cysteine and DL-dithiothreitol displayed similar levels of non-agglutinated spermatozoa at 0 h compared with PEN but were less effective after 3 h of incubation (37 °C). The addition of 10 µM Cu(2+) to 250 µM PEN + TALP caused a rapid reversion of the motile sperm population from a non-agglutinated state to an agglutinated state. Other heavy metals (cobalt, iron, manganese and zinc) did not provoke such a strong response. Together, these results indicate that PEN prevents sperm association by the reduction of disulphide bonds on a sperm membrane protein that binds copper. ADAM proteins are possible candidates, as targeted inhibition of the metalloproteinase domain significantly increased the percentage of motile, non-agglutinated spermatozoa (52.0% ± 7.8) compared with TALP alone (10.6% ± 6.1).

  3. The Staphylococcus aureus ArlRS two-component system is a novel regulator of agglutination and pathogenesis.

    Science.gov (United States)

    Walker, Jennifer N; Crosby, Heidi A; Spaulding, Adam R; Salgado-Pabón, Wilmara; Malone, Cheryl L; Rosenthal, Carolyn B; Schlievert, Patrick M; Boyd, Jeffrey M; Horswill, Alexander R

    2013-01-01

    Staphylococcus aureus is a prominent bacterial pathogen that is known to agglutinate in the presence of human plasma to form stable clumps. There is increasing evidence that agglutination aids S. aureus pathogenesis, but the mechanisms of this process remain to be fully elucidated. To better define this process, we developed both tube based and flow cytometry methods to monitor clumping in the presence of extracellular matrix proteins. We discovered that the ArlRS two-component system regulates the agglutination mechanism during exposure to human plasma or fibrinogen. Using divergent S. aureus strains, we demonstrated that arlRS mutants are unable to agglutinate, and this phenotype can be complemented. We found that the ebh gene, encoding the Giant Staphylococcal Surface Protein (GSSP), was up-regulated in an arlRS mutant. By introducing an ebh complete deletion into an arlRS mutant, agglutination was restored. To assess whether GSSP is the primary effector, a constitutive promoter was inserted upstream of the ebh gene on the chromosome in a wildtype strain, which prevented clump formation and demonstrated that GSSP has a negative impact on the agglutination mechanism. Due to the parallels of agglutination with infective endocarditis development, we assessed the phenotype of an arlRS mutant in a rabbit combined model of sepsis and endocarditis. In this model the arlRS mutant displayed a large defect in vegetation formation and pathogenesis, and this phenotype was partially restored by removing GSSP. Altogether, we have discovered that the ArlRS system controls a novel mechanism through which S. aureus regulates agglutination and pathogenesis.

  4. The Life Cycle of Entzia, an Agglutinated Foraminifer from the Salt Marshes in Transylvania

    Science.gov (United States)

    Kaminski, Michael; Telespan, Andreea; Balc, Ramona; Filipescu, Sorin; Varga, Ildiko; Görög, Agnes

    2013-04-01

    The small salt marshes associated with Miocene salt domes in Transylvania are host to a variety of marine organisms, including communities of halophytic plants as well as an agglutinated foraminifer that is normally found in coastal salt marshes worldwide. Originally described as the species Entzia tetrastoma by Daday (1884), the foraminifer is more widely known by the name Jadammina macrescens (Brady, 1870). Because the genus name Entzia has priority over Jadammina, the valid name of this taxon is Entzia macrescens (Brady, 1870). In 2007, we discovered a living population of Entzia inhabiting a small salt marsh just outside the town of Turda in central Transylvania, only a kilometer from the famous Maria Theresa Salt Mine. This is the first discovery of a living population of Entzia in Transylvania since the species was originally described in 1884. To determine whether or not the specimens we found represent a breeding population, samples were collected from the marsh on a monthly basis over the span of a year. This species can be found among the roots of the halophytic plants, in the uppermost one or two centimeters of the mud. Sediment samples were preserved in Vodka with Rose Bengal to distinguish living and dead specimens, and examined quantitatively. To document the life cycle of the species the following metrics were carried out: test size, abundance, number of chambers, ratio between live and dead specimens, and the diameter of the proloculus. An increase in the mean diameter of specimens was found from October to December. However the mean diameter decreased again in January, which suggests that asexual reproduction had apparently taken place. Small specimens again appeared in March, when sexual reproduction is presumed to have taken place. The median proloculus diameter was smallest in April and May, but the monthly changes in mean proloculus size within the population over the span of a year are not significant. However, specimens with largest

  5. Calculation of cracking moment of wind-proof plate and test study%防风板开裂弯矩计算及试验研究

    Institute of Scientific and Technical Information of China (English)

    王元; 王起才; 郭健; 赵鹏

    2013-01-01

    The property of the cross section influences the cracking moment of the wind-shield plate, which further determines the loading scheme of the static loading test. The paper started the research with the help of the existing calculation method and computational theory,and with a reference to the force-bearing features of the plate and relevant schematic diagram of calculation,a formula for the calculation of cracking moment was eventually concluded. A comparison of the experimental values and calculation values indicates that the formula arrived is of high accuracy and its in-situ application produced effective result.%为了确定防风板静载试验的加载方案,需根据防风板截面特性计算开裂弯矩。本文立足已有开裂弯矩计算方法及计算理论,通过分析防风板的受力特点,根据恰当的应力计算图式,推导了防风板的开裂弯矩计算公式。试验值与计算值对比结果表明,用本文公式计算的开裂弯矩具有可靠的精确度,现场试验的应用效果良好。

  6. Investigation and performance tests of a new parallel plate ionization chamber with double sensitive volume for measuring diagnostic X-rays

    Science.gov (United States)

    Sharifi, B.; Zamani Zeinali, H.; Soltani, J.; Negarestani, A.; Shahvar, A.

    2015-01-01

    Medical diagnostic equipment, like diagnostic radiology and mammography require a dosimeter with high accuracy for dosimetry of the diagnostic X-ray beam. Ionization chambers are suitable instruments for dosimetry of diagnostic-range X-ray beams because of their appropriate response and high reliability. This work introduces the design and fabrication of a new parallel plate ionization chamber with a PMMA body, graphite-coated PMMA windows (0.5 mm thick) and a graphite-foil central electrode (0.1 mm thick, 0.7 g/cm3 dense). This design improves upon the response characteristics of existing designs through the specific choice of materials as well as the appropriate size and arrangement of the ionization chamber components. The results of performance tests conducted at the Secondary Standard Dosimetry laboratory in Karaj-Iran demonstrated the short and long-term stability, the low leakage current, the low directional dependence, and the high ion collection efficiency of the design. Furthermore, the FLUKA Monte Carlo simulations confirmed the low effect of central electrode on this new ionization chamber response. The response characteristics of the parallel plate ionization chamber presented in this work makes the instrument suitable for use as a standard dosimeter in laboratories.

  7. Investigation and performance tests of a new parallel plate ionization chamber with double sensitive volume for measuring diagnostic X-rays

    Energy Technology Data Exchange (ETDEWEB)

    Sharifi, B., E-mail: babak_sharifi88@yahoo.com [Graduate University of Advanced Technology, Kerman (Iran, Islamic Republic of); Zamani Zeinali, H. [Application of Radiation Research School, Nuclear Science and Technology Research Institute, AEOI, Karaj (Iran, Islamic Republic of); Soltani, J.; Negarestani, A. [Graduate University of Advanced Technology, Kerman (Iran, Islamic Republic of); Shahvar, A. [Application of Radiation Research School, Nuclear Science and Technology Research Institute, AEOI, Karaj (Iran, Islamic Republic of)

    2015-01-11

    Medical diagnostic equipment, like diagnostic radiology and mammography require a dosimeter with high accuracy for dosimetry of the diagnostic X-ray beam. Ionization chambers are suitable instruments for dosimetry of diagnostic-range X-ray beams because of their appropriate response and high reliability. This work introduces the design and fabrication of a new parallel plate ionization chamber with a PMMA body, graphite-coated PMMA windows (0.5 mm thick) and a graphite-foil central electrode (0.1 mm thick, 0.7 g/cm{sup 3} dense). This design improves upon the response characteristics of existing designs through the specific choice of materials as well as the appropriate size and arrangement of the ionization chamber components. The results of performance tests conducted at the Secondary Standard Dosimetry laboratory in Karaj-Iran demonstrated the short and long-term stability, the low leakage current, the low directional dependence, and the high ion collection efficiency of the design. Furthermore, the FLUKA Monte Carlo simulations confirmed the low effect of central electrode on this new ionization chamber response. The response characteristics of the parallel plate ionization chamber presented in this work makes the instrument suitable for use as a standard dosimeter in laboratories.

  8. Differential actions of proteinases and neuraminidase on mammalian erythrocyte surface and its impact on erythrocyte agglutination by concanavalin A.

    Science.gov (United States)

    Sharma, Savita; Gokhale, Sadashiv M

    2012-12-01

    Action of proteinases viz. trypsin and chymotrypsin, and neuraminidase on intact erythrocyte membrane proteins and glycophorins (sialoglycoproteins) exposed to cell surface and its impact on lectin (concanavalin A)-mediated agglutination were studied in Homo sapiens (human), Capra aegagrus hircus (goat) and Bubalus bubalis (buffalo). Membrane proteins and glycophorins analysis by SDS-PAGE as visualized by coomassie brilliant blue and periodic acid-schiff stains, respectively, and agglutination behaviour revealed marked differences: 1) there were prominent dissimilarities in the number and molecular weights of glycophorins in human, goat and buffalo erythrocyte membranes; 2) proteinase action(s) on human and buffalo erythrocyte surface membrane proteins and glycophorins showed similarity but was found different in goat; 3) significant differences in erythrocyte agglutinability with concanavalin A can be attributed to differences in membrane composition and alterations in the surface proteins after enzyme treatment; 4) a direct correlation was found between degradation of glycophorins and concanavalin A agglutinability; 5) action of neuraminidase specifically indicated the negative role of cell surface sialic acids in determining concanavalin A agglutinability of goat and buffalo erythrocytes, similar to human. Present studies clearly indicate that there are some basic differences in human, goat and buffalo erythrocyte membrane proteins, especially with respect to glycophorins, which determine the concanavalin A-mediated agglutination in enzyme treated erythrocytes.

  9. Concanavalin A-mediated cell agglutinability induced by Vaccinia virions. [Uv radiation, /sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Mbuy, G.; Bubel, H.C.

    1978-12-01

    The induction of enhanced concanavalin A (Con A)-mediated cellular agglutinability by purified vaccinia virus was examined quantitatively. Increased HEp-2 cell agglutinability by the lectin occurred within the first hour of infection and persisted without further change throughout the virus infectious cycle. Ultraviolet, but not heat-inactivated, virus was as effective as infectious virus in causing increased Con A agglutinability. Inhibition of viral and host cell protein synthesis by Streptovitacin A failed to alter the lectin response to vaccinia virus infection. Fluorescein-labeled Con A was observed to form clusters and large fluorescent patches on the infected cell surface during the earliest stage of infection. Studies with /sup 125/I-labeled Con A revealed an early but minimal increase in lectin binding to infected cells. After the first hour of infection, no further increase in Con A binding was observed. When cells were exposed to purified vaccinia virus surface tubules increased Con A agglutinability comparable to that obtained with native virus was demonstrated. Con A-mediated agglutinability of cells was temperature-dependent and displayed a higher temperature transition in infected cells. These data suggest that upon contact with the host cell, vaccinia virions or surface tubules induce alterations in the plasma membrane which are reflected in an enhanced agglutinability by Con A.

  10. Comparison of a New and Rapid Method: Brucella Coombs Gel Test With Other Diagnostic Tests.

    Science.gov (United States)

    Kalem, Fatma; Ergün, Ayşe Gül; Durmaz, Süleyman; Doğan, Metin; Ertuğrul, Ömür; Gündem, Seval

    2016-09-01

    The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis. Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination tests of 78 patients with presumptive diagnosis of brucellosis which were sent to Microbiology Laboratory of Konya Numune Hospital from various regions of Konya were studied. Of 78 patients with ELISA IgG and IgM, STA, BICA and BCGT; 26, 21, 10, 12 and 12 were positive. When compared with BICA, the sensitivity and specifity of BCGT were 100% and 100%, respectively. According to results BCGT can be used as a diagnostic test in routine laboratories after more comprehensive studies in control groups and patients. © 2016 Wiley Periodicals, Inc.

  11. INL HIP Plate Fabrication

    Energy Technology Data Exchange (ETDEWEB)

    B. H. Park; C. R. Clark; J. F. Jue

    2010-02-01

    This document outlines the process used to bond monolithic fuel plates by Hot Isostatic Pressing (HIP). This method was developed at Idaho National Laboratory (INL) for the Reduced Enrichment for Research and Test Reactors (RERTR) program. These foils have been used in a number of irradiation experiments in support of the United States Global Threat Reduction Initiative (GTRI) program.

  12. Test beam results of micro channel plates in 'ionisation mode' for the detection of single charged particle and electromagnetic showers

    Energy Technology Data Exchange (ETDEWEB)

    Barnyakov, A.; Barnyakov, M. [Budker Institute of Nuclear Physics, pr. Akademika Lavrentieva, 11, Novosibirsk, (Russian Federation); Novosibirsk State University, str. Pirogova 2, Novosibirsk, (Russian Federation); Brianza, L.; Ghezzi, A.; Gotti, C.; Govoni, P.; Martelli, A.; Marzocchi, B.; Pigazzini, S.; Tabarelli de Fatis, T.; Trevisani, N. [Universita di Milano Bicocca and INFN, Sezione di Milano-Bicocca,, Piazza della Scienza 3, I-20126, Milano, (Italy); Cavallari, F.; Del Re, D.; Gelli, S.; Jorda Lope, C.; Meridiani, P.; Organtini, G.; Paramatti, R.; Pernie, L.; Rahatlou, S.; Rovelli, C.; Santanastasio, F. [Universita di Roma ' La Sapienza' and INFN, Sezione di Roma1, P.le A. Moro 1, I-00044 Rome, (Italy)

    2015-07-01

    IMCP is an R and D project aimed at the exploitation of secondary emission of electrons from the surface of microchannel plates (MCP) for fast timing of showers in high rate environments. The usage of MCPs in 'ionisation' mode has long been proposed and is used extensively in ion time-of-flight mass spectrometers. What has not been investigated in depth is their use to detect the ionizing component of showers. The fast time resolution of MCPs exceeds anything that has been previously used in calorimeters, and, if exploited effectively, could aid in the event reconstruction at high luminosities. Results from tests with electrons with energies up to 150 GeV of MCP devices with different characteristics will be presented, in particular detection efficiency and time resolution. (authors)

  13. The effects of diazepam on the behavioral structure of the rat's response to pain in the hot-plate test: anxiolysis vs. pain modulation.

    Science.gov (United States)

    Casarrubea, Maurizio; Sorbera, Filippina; Santangelo, Andrea; Crescimanno, Giuseppe

    2012-08-01

    The aim of the present study was to evaluate, by means of quantitative and multivariate analyses, the effects of diazepam on the behavioral structure of the rat's response to pain in the hot-plate test as well as whether such changes are associated with drug-induced effects on anxiety and/or nociception. To this purpose, ten groups of male Wistar rats were intraperitoneally injected with saline, diazepam (0.25, 0.5 and 2 mg/kg), FG-7142 (1, 4 and 8 mg/kg) or morphine (3, 6 and 12 mg/kg). The mean number and mean latency to first appearance were calculated for each behavioral component. In addition, multivariate cluster and adjusted residual analyses based on the elaboration of transition matrices were performed. Three main behavioral categories were identified: exploratory (walking, sniffing), primary noxious-evoked (hind paw licking, front paw licking, shaking/stamping) and escape (climbing, jumping). Although no significant modifications in the latencies of the primary noxious-evoked components were induced by treatment with diazepam or FG-7142, significant effects were provoked by morphine treatment. Multivariate analyses showed that diazepam-induced anxiolysis redirected the rat's behavior toward a more purposeful and effective escape strategy. In contrast, the high level of anxiety induced by FG-7142 caused the behavioral structure to become disorganized and not purposefully oriented. Changes in the organization of behavioral components were observed in morphine-treated animals and mainly consisted of modifications in the primary noxious-evoked and escape components. The findings suggest that the effects of diazepam on the structure of the rat's response to pain in the hot-plate test are more likely attributable to anxiolysis than pain modulation.

  14. 砂浆板冲击破坏试验研究%Damage tests for a cement mortar plate under shock load

    Institute of Scientific and Technical Information of China (English)

    顾培英; 邓昌; 章道生; 汤雷; 王建

    2015-01-01

    通过锤击、均匀冲击荷载试验,采用逐级递增循环冲击加载方式,研究冲击荷载下砂浆板的破坏特征及冲击力、冲击能与最大加速度响应间关系。试验表明,二种冲击作用均使砂浆板跨中区域出现贯穿裂缝,呈脆性劈裂破坏形态,均匀冲击作用下破坏位置与跨中有一定偏移;锤击力时程经历主冲击、次冲击、卸载三阶段,加速度响应随锤击力增加而增加,裂缝贯穿后冲击力、加速度响应大幅减小;均匀冲击下加速度有二组响应区,响应随冲击能增加而增加,当冲击能达一定程度时响应大幅减小;继续增加冲击能,响应又会增加,并较快发生劈裂破坏,响应大幅减小;支座螺栓松动能缓冲部分冲击作用。%Hammering and uniform shock tests of a cement mortar plate were conducled.The opposite sides of the plate were elastic supports.Through cyclic shocks with amplitude increasing,the failure features,the relationship between shock force and acceleration response,and the relationship between impact energy and acceleration response of the plate were studied.The results showed that a transverse crack nearby the midspan of the plate appears,its failure state is brittle splitting;the location of failure offsets the midspan under uniform shock;time history curves of the hammering force include three stages of major shock,secondary shock and unloading;the acceleration response of the plate increases with increase in the hammering force,however,the hammering force and acceleration response decrease obviously after the transverse crack appears;there are two groups of acceleration response regions under uniform shock;the acceleration response increases with increase in impact energy;once impact energy reaches a certain level,the acceleration response decreases obviously;if impact energy continuously increases,the acceleration response increases again,brittle splitting failure occurs

  15. Standard performance tests of collectors of solar thermal energy: A selectively coated, flat-plate copper collector with one transparent cover and a tube-to-tube spacing of 3-7/8 inches

    Science.gov (United States)

    1976-01-01

    Basic test results are given of a flat-plate solar collector whose performance was determined in the NASA-Lewis solar simulator. The collector was tested over ranges of inlet temperatures, fluxes, and coolant flow rates. Collector efficiency is correlated in terms of inlet temperature and flux level.

  16. Standardized performance tests of collectors of solar thermal energy: A selectively coated, flat-plate copper collector with one transparent cover and a tube-to-tube spacing of 5 5/8 inches

    Science.gov (United States)

    1976-01-01

    This preliminary data report gives basic test results of a flat-plate solar collector whose performance was determined in the NASA-Lewis solar simulator. The collector was tested over ranges of inlet temperatures, fluxes and coolant flow rates. Collector efficiency is correlated in terms of inlet temperature and flux level.

  17. Latex agglutination assays for detection of non-O157 Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145.

    Science.gov (United States)

    Medina, Marjorie B; Shelver, Weilin L; Fratamico, Pina M; Fortis, Laurie; Tillman, Glenn; Narang, Neelam; Cray, William C; Esteban, Emilio; Debroy, Andchitrita

    2012-05-01

    Latex agglutination assays utilizing polyclonal antibodies were developed for the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups. Rabbit antisera were affinity purified through protein A/G columns, and the isolated immunoglobulins (IgGs) were covalently immobilized onto polystyrene latex particles. The resulting latex-IgG complex had a protein (IgG) load of 0.20 to 0.28 mg/ml in a 1% latex suspension. Optimum conditions for the agglutination assay consisted of utilizing 20 μm l of latex-IgG reagent containing 2.0 to 2.8 μm g IgG in a 0.5% latex suspension. Agglutination or flocculation was observed almost instantly after mixing the colonies with the latex-IgG, indicating STEC strains. More than 100 target and nontarget strains were tested in more than 3,000 test replicates. All target organisms produced positive results, but three antisera (anti-O26, anti-O103, and anti-O145) cross-reacted with some other STECs. The anti-O103 and anti-O145 latex reagents cross-reacted with O26 strains, and the anti-O26 cross-reacted with O103 strains. The latex-IgG reagents are stable for at least 1 year and are easy to prepare. These agglutination assays can be used for identification of presumptive non-O157 STEC colonies from agar media. The techniques used to prepare the latex reagents also can be utilized for testing other STEC serogroups, other E. coli serotypes, or other pathogens to ensure safe foods to consumers.

  18. Comparison of the SimPlate coliform and Escherichia coli test with Petrifilm, three-tube MPN, and VRBA + MUG methods for enumerating coliforms and E. coli in food.

    Science.gov (United States)

    Townsend, D E; Irving, R L; Naqui, A

    1998-04-01

    SimPlate for coliforms and Escherichia coli (CEc) is a new method for the detection and quantification of coliforms and E. coli in food. Internal validation of the method was carried out at IDEXX Laboratories (Westbrook, ME) with 180 food samples representing a variety of different food matrices and compared against three-tube MPN (most probable number), VRBA (violet red bile agar) + MUG, and Petrifilm (E. coli count) methods. SimPlate CEc was highly correlated with each of these methods for the quantification of coliform bacteria (r > or = 0.90). An insignificant number of food samples were found to contain E. coli; therefore, no meaningful correlation data could be generated. Four hundred forty-four additional food samples were tested at five collaborating laboratories for the presence of coliforms E. coli using SimPlate CEc and either VRBA + MUG or Petrifilm (E. coli count). Regression analysis of data from SimPlate for CEc versus Petrifilm E. coli count plates generated correlation coefficients (r) of at least 0.89 for total coliforms and at least 0.90 for generic E. coli. Correlation coefficients between SimPlate for CEc and VRBA + MUG data were at least 0.90 for coliforms and at least 0.86 for E. coli. SimPlate for CEc demonstrated better recovery of E. coli than Petrifilm when high populations of bacteria were present. E. coli was not detected in 20 or 50 (40%) raw milk samples tested by the Petrifilm method due to the presence of interfering coliform and noncoliform bacteria. It is concluded that SimPlate for CEc is a suitable alternative for determining numbers of coliform bacteria and E. coli in food.

  19. Create Your Plate

    Medline Plus

    Full Text Available ... A A A Listen En Español Create Your Plate Create Your Plate is a simple and effective ... and that your options are endless. Create Your Plate! Click on the plate sections below to add ...

  20. A novel method for negating cold agglutination interference by dithiothreitol during complete blood count and peripheral blood smear: a case study

    Directory of Open Access Journals (Sweden)

    Hamit Yasar Ellidag

    2017-02-01

    Full Text Available Cold agglutinin disease is an autoimmune disorder that is characterized by antibodies attacking polysaccharide antigens on one’s own erythrocytes. In cold agglutinin disease, hemolysis occurs due to degeneration of erythrocyte membranes and autoagglutination of erythrocytes. Both autoagglutination and hemolysis alter many laboratory test results of the patients, especially complete blood count (CBC and peripheral blood smear analyses. In our laboratory, we have encountered a blood sample of a 65-years-old man, who was independently diagnosed with cold agglutinin disease, and failed to produce meaningful CBC or peripheral blood smear analysis. Fresh blood samples were taken from the patient, and CBC and peripheral blood smear analyses were repeated with and without water bath incubation at 37 °C. Also, varying amounts of dithio­threitol (DTT were added to fresh samples without heat treatment prior to blood analysis. Heat treatment at 37 °C for varying lengths of time failed to improve CBC and peripheral blood smear analysis. On the other hand, addition of DTT into a blood sample of 2 ml in a K3-EDTA tube was sufficient to negate cold agglutination interference during both CBC and peripheral blood smear analyses. The presented method supports the potential for DTT to be used in negating cold agglutination interference during CBC and peripheral blood smear analyses of patients with cold agglutinin disease. The method we describe is very easy and quick with remarkable results.

  1. Spanish Picture Vocabulary Test. Prueba Ilustrada de vocabulario Espanol. An Adaptation of the Peabody Picture Vocabulary Test. Manual [and] Plates. Research Project 1978.

    Science.gov (United States)

    Wiener, Florence D.; And Others

    The Spanish Picture Vocabulary Test was designed to provide a Spanish-language translation of the Peabody Picture Vocabulary Test which would be suitable for Spanish-speaking Puerto Rican school-children in New York City. The 300 stimulus words were translated according to a consensus of linguists and persons of Puerto Rican descent, and were…

  2. Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders

    Directory of Open Access Journals (Sweden)

    R. L. Luciano

    2011-01-01

    Full Text Available Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS. Serum from commercial poultry breeders (=2781 was tested for MS by serum plate agglutination (SPA, hemagglutination inhibition (HI, and enzyme-linked immunosorbent assay (ELISA. From 2,781 samples tested, 736 (26.46% were positive in SPA. From 712 SPA-positive sera, 30 samples (4.21% were positive in HI, and 150 samples (21.06% were positive in ELISA. Copositivity between ELISA and HI was 90%, and conegativity was 82.0%. Agreement between HI and ELISA was rejected by McNemar's test (≤.001, and Kappa coefficient showed a weak correlation between the two techniques (=0.25; 0.21≤<0.40. Weak statistical correlation was observed between all serological tests (SPA, HI, and ELISA, and they should only be used for initial screening for MS.

  3. Kinetics and time dependence of the differential agglutination of acetone [AC]- and formalin [HS]-fixed Toxoplasma tachyzoites by serum of mice with experimental toxoplasmosis.

    Science.gov (United States)

    Ali, Nehad Mahmoud; Habib, Khaled Sayed Mohamed

    2012-04-01

    Researches to specify a serologic diagnostic test capable of determining the stage of toxoplasmosis, whether recent or latent, have been hampered by lack of knowing the real time of infection. Studying the precise kinetics of the differential agglutination of acetone [AC]-fixed versus that of formalin [HS]-fixed tachyzoites (differential agglutination test or AC/HS test) by sera of mice during the course of toxoplasmosis and assessment of its value in the differentiation between recent and latent infections in mice were the aims of the present work. Experimental toxoplasmosis was induced in mice, sera were collected sequentially and AC/HS test, FAST-ELISA to determine levels of IgM and IgG and microscopic examination of brain for Toxoplasma cysts were done. Both AC and HS specific patterns in the AC/HS test were noted to be dependent on the time from the onset of infection. Acute patterns of the AC/HS test were observed early in infection till before the appearance of brain cysts. Non-acute patterns were obtained late on 28th day post infection coinciding with the disappearance of IgM, persistence of IgG and presence of cysts in brains. The AC antibody was high in the recent phase of infection, and then it declined to be replaced by high sustained level of HS antibody late in infection. In conclusion, in the presence of both IgM and IgG, the appearance of either equivocal pattern or the non-acute pattern in the AC/HS test is significant in ruling out acute infection in mice.

  4. Biocompatibility Analyses of Al2O3-Treated Titanium Plates Tested with Osteocyte and Fibroblast Cell Lines

    OpenAIRE

    Smargiassi, Alberto; Bertacchini, Jessika; Checchi, Marta; Cavani, Francesco; Ferretti, Marzia; Palumbo, Carla

    2017-01-01

    Osseointegration of a titanium implant is still an issue in dental/orthopedic implants durable over time. The good integration of these implants is mainly due to their surface and topography. We obtained an innovative titanium surface by shooting different-in-size particles of Al2O3 against the titanium scaffolds which seems to be ideal for bone integration. To corroborate that, we used two different cell lines: MLO-Y4 (murine osteocytes) and 293 (human fibroblasts) and tested the titanium sc...

  5. Nanoscale Mineralogy and Composition of Experimental Regolith Agglutinates Produced under Asteroidal Impact Conditions

    Science.gov (United States)

    Christoffersen, Roy; Cintala, M. J.; Keller, L. P.; See, T. H.; Horz, F.

    2013-01-01

    On the Moon, the energetics of smaller impactors and the physical/chemical characteristics of the granular regolith target combine to form a key product of lunar space weathering: chemically reduced shock melts containing optically-active nanophase Fe metal grains (npFe0) [1]. In addition to forming the optically dark glassy matrix phase in lunar agglutinitic soil particles [1], these shock melts are becoming increasingly recognized for their contribution to optically active patina coatings on a wide range of exposed rock and grain surfaces in the lunar regolith [2]. In applying the lessons of lunar space weathering to asteroids, the potential similarities and differences in regolith-hosted shock melts on the Moon compared to those on asteroids has become a topic of increasing interest [3,4]. In a series of impact experiments performed at velocities applicable to the asteroid belt [5], Horz et al. [6] and See and Horz [7] have previously shown that repeated impacts into a gabbroic regolith analog target can produce melt-welded grain aggregates morphologically very similar to lunar agglutinates [6,7]. Although these agglutinate-like particles were extensively analyzed by electron microprobe and scanning electron microscopy (SEM) as part of the original study [7], a microstructural and compositional comparison of these aggregates to lunar soil agglutinates at sub-micron scales has yet to be made. To close this gap, we characterized a representative set of these aggregates using a JEOL 7600 field-emission scanning electron microscope (FE-SEM), and JEOL 2500SE field-emission scanning transmission electron microscope (FE-STEM) both optimized for energy dispersive X-ray spectroscopy (EDX) compositional spectrum imaging at respective analytical spatial resolutions of 0.5 to 1 micron, and 2 to 4 nm.

  6. Initial Testing for the Recommendation of Improved Gas Metal Arc Welding Procedures for HY-80 Steel Plate Butt Joints at Norfolk Naval Shipyard

    Science.gov (United States)

    2015-12-01

    FOR THE RECOMMENDATION OF IMPROVED GAS METAL ARC WELDING PROCEDURES FOR HY-80 STEEL PLATE BUTT JOINTS AT NORFOLK NAVAL SHIPYARD by Veronika J...FOR THE RECOMMENDATION OF IMPROVED GAS METAL ARC WELDING PROCEDURES FOR HY-80 STEEL PLATE BUTT JOINTS AT NORFOLK NAVAL SHIPYARD 5. FUNDING NUMBERS 6...gas metal arc welding, submarine, hull cut, butt joint, weld, shielding gas, HY-80 steel , plate 15. NUMBER OF PAGES 53 16. PRICE CODE 17

  7. LEPINOCONUS CHIOCCHINII GEN. N., N. SP., A CONICAL AGGLUTINATED FORAMINIFERA FROM THE UPPER CRETACEOUS OF ITALY

    Directory of Open Access Journals (Sweden)

    ERZIKA CRUZ-ABAD

    2017-04-01

    Full Text Available A new conical agglutinated foraminifer, Lepinoconus chiocchinii gen n., n. sp. from the lower Campanian shallow-water platform deposits of the Lepini Mountains (central Apennines, Italy, is described. It has a pseudo-keriothecal wall structure, uniserial arrangement of the adult chambers and multiple apertures. The exoskeleton is constituted by beams (main and intercalary continuous from one chamber to the next, while the endoskeleton bears pillars. The new taxon is included in the Coskinolinidae family. Lepinoconus chiocchinii gen. n., n. sp. is known from southern Italy, Greece and Albania.

  8. Estudio de la quitosana cubana como agente aglutinante Study of Cuban quitosana as agglutination agent

    Directory of Open Access Journals (Sweden)

    Diamelva del Campo Portales

    2007-08-01

    Full Text Available Para evaluar la capacidad como agente aglutinante de la quitosana cubana, derivada de quitina de langosta, se realizó un diseño experimental cuyas variables fueron la relación entre la masa de quitosana y granulado base, así como la concentración de ácido láctico en la solución aglutinante. Los resultados muestran que el porcentaje de ácido láctico no tiene influencia en las propiedades de los granulados elaborados con quitosana. La relación quitosana/granulado base influye sobre el tamaño de partícula promedio de los granulados estudiados. Las propiedades de los granulados elaborados con quitosana, independientemente de la relación de quitosana/granulado base y el porcentaje de ácido láctico, tienen un comportamiento intermedio entre la polivinilpirrolidona y la gelatina. Con el fármaco modelo sulfato de cloroquina, al emplear la quitosana cubana como agente aglutinante se obtuvieron tabletas con adecuadas propiedades. Adicionalmente se comparó su efectividad como aglutinante con respecto a excipientes de uso tradicional: gelatina, polivinilpirrolidona y celacol, con el clorhidrato de propranolol como fármaco modelo. Los granulados obtenidos muestran que la quitosana al 1 % tiene un comportamiento próximo al polivinilpirrolidona, celacol y gelatina, mientras que para las tabletas, independientemente del valor de la concentración de la quitosana, es similar al polivinilpirrolidona.To assess ability of Cuban quitosona as agglutination agent, derived from lobster quitine, we performed an experimental design of which he variable were: relationship between quitosana mass and granular base as well as lactic acid concentration in agglutination solution. Results shows that lactic acid percentage hasn’t influence on properties of granules processed using quitosana. Above mentioned relationship influences on average particle size of study granules. Properties of quitosana-processed granules, regardless of whether quitosana

  9. Evolution of magma feeding system in Kumanodake agglutinate activity, Zao Volcano, northeastern Japan

    Science.gov (United States)

    Takebe, Yoshinori; Ban, Masao

    2015-10-01

    The Kumanodake agglutinate of Zao Volcano in northeastern Japan consists of pyroclastic surge layers accumulated during the early part of the newest stage of activity (ca. 33 ka to present). Our petrologic study of this agglutinate based on systematically collected samples aims to reveal the evolution of magma feeding system. To understand the magma evolution, we have examined samples from the agglutinate by using petrologic data including, petrography, analysis of minerals (plagioclase, pyroxene, and olivine), glass compositions, and whole rock major element and trace element (Ba, Sr, Cr, Ni, V, Rb, Zr, Nb, and Y) compositions. Agglutinate are mixed, medium-K, calc-alkaline olv-cpx-opx basaltic andesite (55.2-56.2% SiO2). Results show that the magma feeding system comprised a shallow felsic chamber injected by mafic magma from depth. The felsic magma (59-62% SiO2, 950-990 °C), which was stored at a shallower depth, had orthopyroxene (Mg# = 60-69), clinopyroxene (Mg# = 65-71), and low-An plagioclase (Anca. 58-70). The mafic magma is further divisible into two types: less-differentiated and more-differentiated, designed respectively as an initial mafic magma-1 and a second mafic magma-2. The original mafic magma-1 was olivine (Fo 84) basalt (ca. 48-51% SiO2, 1110-1140 °C). The second mafic magma-2, stored occasionally at 4-6 km depth, was basalt (1070-1110 °C) having Foca. 80 olivine and high-An (Anca. 90) plagioclase phenocrysts. These two magmas mixed (first mixing) to form hybrid mafic magma. The forced injections of the hybrid mafic magmas activated the felsic magma, and these two were mixed (second mixing) shortly before eruptions. The explosivity is inferred to have increased over time because the abundance of large scoria increased. Furthermore, the erupted magma composition became more mafic, which reflects increased percentage of the hybrid mafic magma involved in the second mixing. At the beginning of activity, the mafic magma also acted as a heat

  10. Pliocene and Pleistocene chronostratigraphy and paleoenvironment of the Central Arctic Ocean, using deep water agglutinated foraminifera

    OpenAIRE

    Evans, J. R.; Kaminski, M.A

    1998-01-01

    Deep-water agglutinated foraminifera (DWAF) were studied from Cores PS2177-5, PS2200-5, PS2212-3 and PS2185-6; from the R/V POLARSTERN ARK-VIII/3 Cruise in the central Arctic Ocean. The sediments were non-calcareous containing a sparse assemblage of eleven DWAF species. A chronostratigraphic framework is presented for Cores PS2200-5 and PS2185-6. Paleoenvironmental data suggests a bathyal environment (2000-4000m) affected by water masses in the Arctic Ocean. The taxonomy of all of the DWAF fo...

  11. Partial inhibition of hemocyte agglutination by Lathyrus odoratus lectin in Crassotrea virginica infected with Perkinsus marinus

    Directory of Open Access Journals (Sweden)

    Thomas C. Cheng

    1995-06-01

    Full Text Available Quantitative determinations of agglutination of hemocytes from oysters, Crassostrea virginica, by the Lathyrus odoratus lectin at five concentrations revealed that clumping of hemocytes from oysters infected with Perkinsus marinus is partially inhibited. Although the nature of the hemocyte surface saccharide, which is not D(+-glucose, D(+mannose, or alpha-methyl-D-mannoside, remains to be determined, it may be concluded that this molecule also occurs on the surface of P. marinus. It has been demonstrated that the panning technique (Ford et al. 1990 is qualitatively as effective for determining the presence of P. marinus in C. virginica as the hemolymph assay method (Gauthier & Fisher 1990.

  12. Evaluation of the limulus amoebocyte lysate test in conjunction with a gram negative bacterial plate count for detecting irradiation of chicken

    Science.gov (United States)

    Scotter, Susan L.; Wood, Roger; McWeeny, David J.

    A study to evaluate the potential of the Limulus amoebocyte lysate (LAL) test in conjuction with a Gram negative bacteria (GNB) plate count for detecting the irradiation of chicken is described. Preliminary studies demonstrated that chickens irradiated at an absorbed dose of 2.5 kGy could be differentiated from unirradiated birds by measuring levels of endotoxin and of numbers of GNB on chicken skin. Irradiated birds were found to have endotoxin levels similar to those found in unirradiated birds but significantly lower numbers of GNB. In a limited study the test was found to be applicable to birds from different processors. The effect of temperature abuse on the microbiological profile, and thus the efficacy of the test, was also investigated. After temperature abuse, the irradiated birds were identifiable at worst up to 3 days after irradiation treatment at the 2.5 kGy level and at best some 13 days after irradiation. Temperature abuse at 15°C resulted in rapid recovery of surviving micro-organisms which made differentiation of irradiated and unirradiated birds using this test unreliable. The microbiological quality of the bird prior to irradiation treatment also affected the test as large numbers of GNB present on the bird prior to irradiation treatment resulted in larger numbers of survivors. In addition, monitoring the developing flora after irradiation treatment and during subsequent chilled storage also aided differentiation of irradiated and unirradiated birds. Large numbers of yeasts and Gram positive cocci were isolated from irradiated carcasses whereas Gram negative oxidative rods were the predominant spoilage flora on unirradiated birds.

  13. High influx of carbon in walls of agglutinated foraminifers during the Permian-Triassic transition in global oceans

    Science.gov (United States)

    Nestell, Galina P.; Nestell, Merlynd K.; Ellwood, Brooks B.; Wardlaw, Bruce R.; Basu, Asish R.; Ghosh, Nilotpal; Phuong Lan, Luu Thi; Rowe, Harry D.; Hunt, Andrew G.; Tomkin, Jonathan H.; Ratcliffe, Kenneth T.

    2015-01-01

    The Permian–Triassic mass extinction is postulated to be related to the rapid volcanism that produced the Siberian flood basalt (Traps). Unrelated volcanic eruptions producing several episodes of ash falls synchronous with the Siberian Traps are found in South China and Australia. Such regional eruptions could have caused wildfires, burning of coal deposits, and the dispersion of coal fly ash. These eruptions introduced a major influx of carbon into the atmosphere and oceans that can be recognized in the wallstructure of foraminiferal tests present in survival populations in the boundary interval strata. Analysis of free specimens of foraminifers recovered from residues of conodont samples taken at aPermian–Triassic boundary section at Lung Cam in northern Vietnam has revealed the presence of a significant amount of elemental carbon, along with oxygen and silica, in their test wall structure, but an absence of calcium carbonate. These foraminifers, identified as Rectocornuspira kalhori, Cornuspira mahajeri, and Earlandia spp. and whose tests previously were considered to be calcareous, are confirmed to be agglutinated, and are now referred to as Ammodiscus kalhori and Hyperammina deformis. Measurement of the 207Pb/204Pb ratios in pyrite clusters attached to the foraminiferal tests confirmed that these tests inherited the Pb in their outer layer from carbon-contaminated seawater. We conclude that the source of the carbon could have been either global coal fly ash or forest fire-dispersed carbon, or a combination of both, that was dispersed into the Palaeo-Tethys Ocean immediately after the end-Permian extinction event.

  14. The false sero-negativity of standard agglutination tests in brucellosis

    Directory of Open Access Journals (Sweden)

    Sevil Bilir Goksugur

    2014-03-01

    Full Text Available 1. Aslan A, Kurtoglu U, Akca MO, Tan S, Soylu U, Aslan M. Cervical brucellar spondylodiscitis mimicking a cervical disc herniation with epidural abscess: a case report. Acta Med Anatol. doi:http://dx.doi.org/10.15824/actamedica.35316

  15. Comparison of Coombs' and immunocapture-agglutination tests in the diagnosis of brucellosis

    Institute of Scientific and Technical Information of China (English)

    Nurittin Ardic; Mustafa Ozyurt; Ogun Sezer; Ali Erdemoglu; Tuncer Haznedaroglu

    2005-01-01

    @@ Brucellosis is an important zoonotic disease caused by bacteria of the genus Brucella encountered in animals such as cows, sheep, goats and pigs as well as in humans. It is one of the most widely seen infections and nearly half a million cases are declared annually. Endemic infections occur especially in the Mediterranean, Middle East, Latin America and Asia.1 Seropositiveness ratios vary between 2% and 12% in Turkey.2 The average annual number of cases declared to the Turkish Ministry of Health between 1991 and 2000 was 9000.3

  16. 9 CFR 147.1 - The standard tube agglutination test. 1

    Science.gov (United States)

    2010-01-01

    ... clotting, and unless they reach the laboratory within a few hours, to pack them with ice in special... consistently. (c) A medium which has been used satisfactorily has the following composition: Water 1,000 cc... percent) saline (0.85 percent) solution to make a heavy suspension. The suspension should be filtered...

  17. Biocompatibility Analyses of Al2O3-Treated Titanium Plates Tested with Osteocyte and Fibroblast Cell Lines

    Science.gov (United States)

    Smargiassi, Alberto; Bertacchini, Jessika; Checchi, Marta; Cavani, Francesco; Ferretti, Marzia; Palumbo, Carla

    2017-01-01

    Osseointegration of a titanium implant is still an issue in dental/orthopedic implants durable over time. The good integration of these implants is mainly due to their surface and topography. We obtained an innovative titanium surface by shooting different-in-size particles of Al2O3 against the titanium scaffolds which seems to be ideal for bone integration. To corroborate that, we used two different cell lines: MLO-Y4 (murine osteocytes) and 293 (human fibroblasts) and tested the titanium scaffolds untreated and treated (i.e., Al2O3 shot-peened titanium surfaces). Distribution, density, and expression of adhesion molecules (fibronectin and vitronectin) were evaluated under scanning electron microscope (SEM) and confocal microscope (CM). DAPI and fluorochrome-conjugated antibodies were used to highlight nuclei, fibronectin, and vitronectin, under CM; cell distribution was analyzed after gold-palladium sputtering of samples by SEM. The engineered biomaterial surfaces showed under SEM irregular morphology displaying variously-shaped spicules. Both SEM and CM observations showed better outcome in terms of cell adhesion and distribution in treated titanium surfaces with respect to the untreated ones. The results obtained clearly showed that this kind of surface-treated titanium, used to manufacture devices for dental implantology: (i) is very suitable for cell colonization, essential prerequisite for the best osseointegration, and (ii) represents an excellent solution for the development of further engineered implants with the target to obtain recovery of stable dental function over time. PMID:28621746

  18. Antimicrobial action and cell agglutination by the eosinophil cationic protein are modulated by the cell wall lipopolysaccharide structure.

    Science.gov (United States)

    Pulido, David; Moussaoui, Mohammed; Andreu, David; Nogués, M Victòria; Torrent, Marc; Boix, Ester

    2012-05-01

    Antimicrobial proteins and peptides (AMPs) are essential effectors of innate immunity, acting as a first line of defense against bacterial infections. Many AMPs exhibit high affinity for cell wall structures such as lipopolysaccharide (LPS), a potent endotoxin able to induce sepsis. Hence, understanding how AMPs can interact with and neutralize LPS endotoxin is of special relevance for human health. Eosinophil cationic protein (ECP) is an eosinophil secreted protein with high activity against both Gram-negative and Gram-positive bacteria. ECP has a remarkable affinity for LPS and a distinctive agglutinating activity. By using a battery of LPS-truncated E. coli mutant strains, we demonstrate that the polysaccharide moiety of LPS is essential for ECP-mediated bacterial agglutination, thereby modulating its antimicrobial action. The mechanism of action of ECP at the bacterial surface is drastically affected by the LPS structure and in particular by its polysaccharide moiety. We have also analyzed an N-terminal fragment that retains the whole protein activity and displays similar cell agglutination behavior. Conversely, a fragment with further minimization of the antimicrobial domain, though retaining the antimicrobial capacity, significantly loses its agglutinating activity, exhibiting a different mechanism of action which is not dependent on the LPS composition. The results highlight the correlation between the protein's antimicrobial activity and its ability to interact with the LPS outer layer and promote bacterial agglutination.

  19. Enzymatic cleavage of cell surface proteins of pig and cow erythrocytes and its effect on concanavalin-mediated agglutinability.

    Science.gov (United States)

    Sharma, Savita; Gokhale, Sadashiv M

    2014-10-01

    Study was carried out to understand and compare architecture of the proteins of erythrocyte cell surface of some mammals viz., Homo sapiens (human), Sus scorfa domestica (pig) and Bos taurus domestica (cow). In this study, we investigated the action of proteinases viz., trypsin and chymotrypsin and neuraminidase on the erythrocyte surface proteins and erythrocyte agglutination tendency with a lectin (concanavalin A). The electrophoretic pattern of membrane proteins and glycophorins (analyzed by SDS-PAGE and visualized by Coomassie brilliant blue and periodic acid-schiff stains, respectively) and concanavalin A (Con A) agglutinability revealed that: (i) There were variations in the number and molecular weights of glycophorins in human, pig and cow, (ii) trypsin action on pig and cow erythrocyte membrane proteins was similar, unlike human, (iii) glycophorins degradation by trypsin and chymotrypsin was not similar in pig, as compared to that of human and cow, (iv) erythrocytes agglutination with Con A was significantly different due to differences in membrane composition and alterations in the surface proteins after enzyme treatment, (v) a direct correlation was found between degradation of glycophorins and Con A agglutinability, and (vi) removal of erythrocyte surface sialic acids by neuraminidase specifically indicated an increase in Con A agglutinability of pig and cow erythrocytes, similar to human.

  20. Isolation of phages for phage therapy: a comparison of spot tests and efficiency of plating analyses for determination of host range and efficacy.

    Science.gov (United States)

    Khan Mirzaei, Mohammadali; Nilsson, Anders S

    2015-01-01

    Phage therapy, treating bacterial infections with bacteriophages, could be a future alternative to antibiotic treatment of bacterial infections. There are, however, several problems to be solved, mainly associated to the biology of phages, the interaction between phages and their bacterial hosts, but also to the vast variation of pathogenic bacteria which implies that large numbers of different phages are going to be needed. All of these phages must under present regulation of medical products undergo extensive clinical testing before they can be applied. It will consequently be of great economic importance that effective and versatile phages are selected and collected into phage libraries, i.e., the selection must be carried out in a way that it results in highly virulent phages with broad host ranges. We have isolated phages using the Escherichia coli reference (ECOR) collection and compared two methods, spot testing and efficiency of plating (EOP), which are frequently used to identify phages suitable for phage therapy. The analyses of the differences between the two methods show that spot tests often overestimate both the overall virulence and the host range and that the results are not correlated to the results of EOP assays. The conclusion is that single dilution spot tests cannot be used for identification and selection of phages to a phage library and should be replaced by EOP assays. The difference between the two methods can be caused by many factors. We have analysed if the differences and lack of correlation could be caused by lysis from without, bacteriocins in the phage lysate, or by the presence of prophages harbouring genes coding for phage resistance systems in the genomes of the bacteria in the ECOR collection.

  1. A Piezoelectric Immunosensor Based on Agglutination Reaction with Amplification of Silica Nanoparticles

    Institute of Scientific and Technical Information of China (English)

    JIN,Xiao-Yong; JIN,Xue-Fang; DING,Yan-Jun; JIANG,Jian-Hui; SHEN,Guo-Li; Ru-Qin

    2008-01-01

    A simple piezoelectric immunoagglutination assay technique with antibody-modified nanoparticles has been developed for direct quantitative detection of protein. The proposed technique is based on the specific agglutination of goat anti-hlgG-coated silica (or gold) nanoparticles in the presence of human immunoglobulin G (hlgG), which causes a frequency change and is monitored by a piezoelectric device. The antibody modified on the probe surface would combine with antibody-coated nanoparticles in the presence of antigen (hlgG) when the surface agglutination reaction took place, which couples both the mass effect and viscoelastic effect acting on the probe. The results indi-cate that the background interference can be substantially minimized and the probe signal can be observably multi-plied. In addition, the surface of the modified probe and that after combining the complex of immunoagglutination were imaged by scanning electronic microscopy (SEM). Moreover, an optimization of assay medium composition with the addition of poly(ethylene glycol) (PEG) serving as immunoagglutination enhancer and sodium chloride to control the ion-strength was investigated. The frequency responses of the immunoagglutination assay were found to correlate well with the hlgG concentration with a detection limit of 84 ng.mL-1.

  2. Systematic updates of the agglutinated foraminiferal genus Colominella Popescu, 1998: insights from sectioned specimens

    Science.gov (United States)

    Mancin, Nicoletta; Kaminski, Michael A.

    2017-04-01

    The occurrence of agglutinated foraminiferal specimens belonging to the Badenian (middle Miocene) genus Colominella Popescu, 1998 was recently documented for the first time in a lower Pliocene succession of the western Mediterranean area. Direct comparisons with topotype specimens of Colominella paalzowi (Cushman 1936), sampled in the Badenian type section of Lăpugiu de Sus (Transylvania), show that the Pliocene individuals from the western Mediterranean morphologically resemble the type species C. paalzowi, but they also differ in possessing a longer biserial chamber arrangement with a higher number of internal chamber partitions, in lacking a clear early triserial stage and in having a more complex microstructure of the agglutinated wall, thereby supporting the idea that the Pliocene Mediterranean specimens represent a new, more highly evolved species. The fact that the Pliocene individuals from the Mediterranean appear to be more evolved with respect to the Badenian specimens from Paratethys represents an interesting evolutionary development of the genus Colominella that also permits the known stratigraphical and geographical range of the genus, previously limited to the middle Miocene (Badenian) of the Paratethys, to be extended.

  3. Comparison of serological tests for the detection of ovine and caprine antibody to Brucella melitensis.

    Science.gov (United States)

    Nielsen, K; Gall, D; Smith, P; Balsevicius, S; Garrido, F; Ferrer, M Durán; Biancifiori, F; Dajer, A; Luna, E; Samartino, L; Bermudez, R; Moreno, F; Renteria, T; Corral, A

    2004-12-01

    The indirect enzyme-linked immunosorbent assay (IELISA), the competitive enzyme-linked immunosorbent assay (CELISA) and the fluorescence polarisation assay (FPA) were evaluated with sera from sheep experimentally infected with Brucella melitensis and negative Canadian sheep. The sensitivity and specificity of the assays were as follows: IELISA: 91.7% and 97.6%, CELISA: 75.0% and 99.8% and FPA: 91.7% and 89.5%. Sera from the same experimental population were divided according to serological reaction in the rose bengal agglutination test (RBT) and the complement fixation test (CFT). Reactivity relative to the RBT positive and CFT positive sera were as follows: IELISA: 99.7%, CELISA: 93.2% and FPA: 99.1%. Since sera from goats with proven B. melitensis infection were not available, 699 sera from goats judged positive in the buffered antigen plate agglutination test (BPAT) and CFT and 982 BPAT/CFT negative Canadian goats were used. The sensitivity and specificity of the assays relative to the BPAT and CFT positive sera were: IELISA: 99.4% and 98.0%, CELISA: 95.4% and 97.1% and FPA: 92.7% and 99.8%.

  4. Detección de anticuerpos anti-Brucella spp. en cerdos mediante técnicas de aglutinación y ELISA indirecto en las provincias de Buenos Aires y La Pampa: Argentina Detection of anti-Brucella spp. antibodies in swine by agglutination techniques and indirect ELISA in the Buenos Aires and La Pampa provinces: Argentina

    Directory of Open Access Journals (Sweden)

    H.A. Castro

    2006-04-01

    Full Text Available En nuestro país no existe un programa de control sobre brucelosis porcina y su verdadera situación epidemiológica es desconocida. El objetivo de nuestro trabajo fue detectar la presencia de anticuerpos anti-Brucella spp. en porcinos provenientes de criaderos del sudoeste de la provincia de Buenos Aires y del este de la provincia de La Pampa. La toma de muestras de sangre se realizó en el momento del faenado de los animales. La detección de anticuerpos se efectuó mediante las técnicas de aglutinación con antígeno tamponado en placa (BPA, seroaglutinación en tubo (SAT, aglutinación con 2-ME (2-ME y ELISA indirecto, con dos antígenos diferentes: el antígeno CYT (fracción citoplasmática de B. abortus S19 y el antígeno CP (extracto citoplasmático libre de lipopolisacárido. Del total de las muestras analizadas (n=325, el 17,8% fue positivo para BPA, el 13,8% fue positivo para SAT y sólo el 8,0% fue positivo para 2-ME. Mediante ELISA-CYT, este porcentaje se elevó a 21,0%, mientras que a través del ELISA-CP sólo se halló un 10,0% de muestras reactivas. Estos resultados son compatibles con los informados en los escasos reportes previos para todo el país y sugieren la necesidad de extender los estudios a otras zonas, donde sea habitual la cría de cerdos.Porcine brucellosis is one of the most important zoonoses in this country. Currently, there is no control program for porcine brucellosis in Argentina and the epidemiological situation is still unknown. The purpose of our study was to detect anti-Brucella spp. antibodies in swine in the southwest of the Buenos Aires province and the east of the La Pampa province. Blood samples were obtained when animals were slaughtered. The presence of anti-brucella antibodies was studied by the buffered plate agglutination test (BPA, the tube agglutination test (SAT, the 2-mercaptoethanol (2-ME agglutination test and indirect ELISA tests, using the cytosolic fraction from Brucella abortus S19

  5. Comparison of the compact dry TC method with the standard pour plate method (AOAC official method 966.23) for determining aerobic colony counts in food samples: Performance-tested method.

    Science.gov (United States)

    Kodaka, Hidemasa; Mizuochi, Shingo; Teramura, Hajime; Nirazuka, Tadanobu

    2005-01-01

    Compact Dry TC qualifies as a rapid method kit for determining aerobic colony counts in foods. The plates are presterilized and contain culture medium and a cold-soluble gelling agent. The medium is rehydrated by inoculating 1 mL diluted sample into the center of the self-diffusible medium and allowing the solution to diffuse by capillary action. The plates can then be incubated and the colonies counted without any additional steps. The Compact Dry TC method was validated with 5 different raw meats. The performance tests were conducted at 35 degrees and 30 degrees C. In all required performance studies, no apparent differences were observed between the Compact Dry TC method and the Standard Pour Plate method (AOAC Official Method 966.23) for the detection level of aerobic microorganisms. For the accuracy claim (n = 60), a correlation factor of r2(35) = 0.9977 (35 degrees C) and r2(30) = 0.9932 (30 degrees C) could be assigned, as stated in the application for "Performance Tested Method." Quality consistency and storage robustness studies, showed no significant variations in plate count results with different production lots or plates of diverse storage age.

  6. Metallographic studies of electron beam welded copper plates. EBSD studies of the cross-sections and determination of EBSD reference curves by EB-welded tensile test samples

    Energy Technology Data Exchange (ETDEWEB)

    Karhula, T. [Tampere Univ. of Technology (Finland)

    2013-07-15

    This work is part of Posiva's spent nuclear fuel disposal canister sealing development. Posiva has welded series of plate welding experiments at Patria Aviation Facilities. At Tampere University of Technology, Department of Materials Science (TUT DMS) metallographic and electron microscopy studies of electron beam welded copper samples have been carried out. In this report a part of the welding test program is analyzed. The results of the cross-sections of the test welds X436-X440 and X453-X458 are presented here together with the methods used. These two sets of welds were conducted to study the effects of welding speed, annealing temperature and the presence of cosmetic pass. The aim of this study was to estimate the residual stresses present in the EB-welds using electron backscatter diffraction (EBSD) technique. For this task various EBSD reference curves with tensile test samples were constructed: the recrystallized, substructured and deformed fractions of grains, the occurrence of 1.5 deg C and 2.5 deg C misorientations, the average of mean intra-grain misorientation AMIS, and the hardness could be related to the applied strain. It was found that the hardness was higher if there was a cosmetic pass on the weld. The welding speed and the annealing temperature did not seem to have a noticeable effect on the measured properties. The estimated residual stresses were mainly very low, in the range 27-34 MPa. In the values estimated based on the hardness reference curve, the maximum residual stress was 58.7 MPa (in X455A). (orig.)

  7. Comparison of optomagnetic and AC susceptibility readouts in a magnetic nanoparticle agglutination assay for detection of C-reactive protein

    DEFF Research Database (Denmark)

    Fock, Jeppe; Parmvi, Mattias; Strömberg, Mattias

    2017-01-01

    conjugated with CRP antibodies. Both methods detect agglutination as a shift to lower frequencies in measurements of the dynamics in response to an applied oscillating magnetic field. The magnetic susceptibility method probes the magnetic response whereas the optomagnetic technique probes the modulation...... of laser light transmitted through the sample. The two techniques provided highly correlated results upon agglutination when they measure the decrease of the signal from the individual MNPs (turn-off detection strategy), whereas the techniques provided different results, strongly depending on the read...

  8. Seismic ACROSS Transmitter Installed at Morimachi above the Subducting Philippine Sea Plate for the Test Monitoring of the Seismogenic Zone of Tokai Earthquake not yet to Occur

    Science.gov (United States)

    Kunitomo, T.; Kumazawa, M.; Masuda, T.; Morita, N.; Torii, T.; Ishikawa, Y.; Yoshikawa, S.; Katsumata, A.; Yoshida, Y.

    2008-12-01

    Here we report the first seismic monitoring system in active and constant operation for the wave propagation characteristics in tectonic region just above the subducting plate driving the coming catastrophic earthquakes. Developmental works of such a system (ACROSS; acronym for Accurately Controlled, Routinely Operated, Signal System) have been started in 1994 at Nagoya University and since 1996 also at TGC (Tono Geoscience Center) of JAEA promoted by Hyogoken Nanbu Earthquakes (1995 Jan.17, Mj=7.3). The ACROSS is a technology system including theory of signal and data processing based on the brand new concept of measurement methodology of Green function between a signal source and observation site. The works done for first generation system are reported at IWAM04 and in JAEA report (Kumazawa et al.,2007). The Meteorological Research Institute of JMA has started a project of test monitoring of Tokai area in 2004 in corporation with Shizuoka University to realize the practical use of the seismic ACROSS for earthquake prediction researches. The first target was set to Tokai Earthquake not yet to take place. The seismic ACROSS transmitter was designed so as to be appropriate for the sensitive monitoring of the deep active fault zone on the basis of the previous technology elements accumulated so far. The ground coupler (antenna) is a large steel-reinforced concrete block (over 20m3) installed in the basement rocks in order to preserve the stability. Eccentric moment of the rotary transmitter is 82 kgm at maximum, 10 times larger than that of the first generation. Carrier frequency of FM signal for practical use can be from 3.5 to 15 Hz, and the signal phase is accurately controlled by a motor with vector inverter synchronized with GPS clock with a precision of 10-4 radian or better. By referring to the existing structure model in this area (Iidaka et al., 2003), the site of the transmitting station was chosen at Morimachi so as to be appropriate for detecting the

  9. An analysis of application effect of brucellin intracutaneous test and bacterial isolation in diagnosis of brucellosis%皮内变态反应试验和分离细菌在布鲁杆菌病诊断中的应用效果分析

    Institute of Scientific and Technical Information of China (English)

    宋利桃; 米景川; 尉瑞平

    2012-01-01

    Objective To evaluate application effect of brucellin intracutaneous test and bacterial isolation on diagnosis of brucellosis. Methods To use tube agglutination test, the rose bengal plate test, brucellin intracutaneous test and bacterial isolation to test 55 patients at the same time . Results To separate the brucella from the possible negative patients' blood that was tested in tube agglutination test. The application of tube agglutination , rose bengal plate test, skin - changing reaction and bacterial isolation contributes to the diagnosis of brucellosis . Conclusion The separation of brucella from the possible negative patients' blood that was tested in tube agglutination test plays important role in the diagnosis of brucellosis patients. Brucellin intracutaneous test helps to diagnose chronic brucellosis.%目的 评价皮内变态反应试验和分离细菌在布鲁杆菌病诊断中的应用效果.方法 运用试管凝集试验、虎红平板凝集试验、布鲁杆菌素皮内变态反应试验、分离细菌的方法同时对55例患者进行检测.结果 在试管凝集试验可疑和阴性的患者血液中分离出布鲁杆菌.检测布鲁杆菌病运用试管凝集试验、虎红平板凝集的同时再加上皮内变态反应 试验和分离细菌的方法有助于布鲁杆菌病的诊断.结论 在试管凝集试验可疑和阴性患者的血液中分离出了布鲁杆菌,对难确诊的布鲁杆菌病患者起着重要作用.皮内变态反应试验有助于慢性布鲁杆菌病的诊断.

  10. Collaborative guarded-hot-plate tests between the Laboratoire national de métrologie et d’essais and the National Institute of Standards and Technology

    Science.gov (United States)

    Zarr, Robert R.; Guthrie, William F.; Hay, Bruno; Koenen, Alain

    2017-02-01

    A collaborative study to compare the long-term measurement performance between guarded-hot-plate facilities at the Laboratoire national de métrologie et d’essais (LNE) in France and the National Institute of Standards and Technology (NIST) in the United States is presented. Thermal conductivity data were compiled from three international comparisons organized from 1997 to 2014. Measurements were conducted in accordance with standardized test methods (ISO 8302 or ASTM C 177) over a temperature range from 280 K to 320 K. Nine thermal insulating materials (either mineral fiber or expanded polystyrene) were examined covering broad ranges of bulk densities (13 kg · m-3-200 kg · m-3) and thicknesses (13 mm-70 mm). A different set of specimens was utilized for each comparison. Results of this study indicate that, over a 17 year interval, the majority of test data from LNE and NIST agreed to within  ±1.0%, or less, for mineral fiber materials and to within  ±0.5%, or less, for expanded polystyrene. The long-term variability limit of 1% between the two laboratories is in good agreement with their current measurement uncertainties. Regression coefficients and their standard uncertainties for a straight-line model relating thermal conductivity to temperature from 280 K to 320 K were computed by material and laboratory. Graphical analysis of the data and corresponding fits exhibit consistent behavior by material type between the two laboratories. Sources of measurement variability are addressed.

  11. Simulation, construction and testing of a two-cylinder solar Stirling engine powered by a flat-plate solar collector without regenerator

    Energy Technology Data Exchange (ETDEWEB)

    Tavakolpour, Ali Reza; Zomorodian, Ali [Department of Mechanics of Farm Machinery Engineering, Shiraz University, Shiraz (Iran); Akbar Golneshan, Ali [Department of Mechanical Engineering, Shiraz University, Shiraz (Iran)

    2008-01-15

    In this research, a gamma-type, low-temperature differential (LTD) solar Stirling engine with two cylinders was modeled, constructed and primarily tested. A flat-plate solar collector was employed as an in-built heat source, thus the system design was based on a temperature difference of 80{sup o}C. The principles of thermodynamics as well as Schmidt theory were adapted to use for modeling the engine. To simulate the system some computer programs were written to analyze the models and the optimized parameters of the engine design were determined. The optimized compression ratio was computed to be 12.5 for solar application according to the mean collector temperature of 100{sup o}C and sink temperature of 20{sup o}C. The corresponding theoretical efficiency of the engine for the mentioned designed parameters was calculated to be 0.012 for zero regenerator efficiency. Proposed engine dimensions are as follows: power piston stroke 0.044 m, power piston diameter 0.13 m, displacer stroke 0.055 m and the displacer diameter 0.41 m. Finally, the engine was tested. The results indicated that at mean collector temperature of 110{sup o}C and sink temperature of 25{sup o}C, the engine produced a maximum brake power of 0.27 W at 14 rpm. The mean engine speed was about 30 rpm at solar radiation intensity of 900 W/m{sup 2} and without load. The indicated power was computed to be 1.2 W at 30 rpm. (author)

  12. Thin plate neotectonic models of the Australian plate

    Science.gov (United States)

    Burbidge, D. R.

    2004-10-01

    Thin plate finite element models of the neotectonic deformation of the Australian plate have been calculated in order to estimate the stress and strain rate within the plate, specifically concentrating on the Australian continent. The model includes plate-bounding faults, an anelastic brittle-ductile layered rheology and the option of laterally varying elevation and heat flow. The results of the models are compared to (1) the velocity of geodetic benchmarks on the Australian plate, (2) the spreading rate of the mid-oceanic ridges along the Australian plate's margins, (3) the direction of the maximum horizontal principal stress, (4) the stress regime within the plate, and (5) the crustal thickness estimated from the depth to the base of Mohorovicic discontinuity's transition zone. A variety of models are tested with a wide range of input parameters. The model with the smallest misfit with observations predicts that the strain rate for most of the Australian continent is approximately 10-17 s-1. This model has a slightly lower strain rate in the central Australia and is higher off the northern coast of Australia than for the rest of the continent. Strain rates of this magnitude would be difficult to observe from geodetic or geologic data for most parts of Australia but would be enough to generate much of the seismicity that has been observed over the last century.

  13. Agglutinated foraminifera from the Northern Tarcău Nappe (Eastern Carpathians, Romania

    Directory of Open Access Journals (Sweden)

    Raluca Bindiu

    2011-10-01

    Full Text Available The Tarcău Nappe is the most important unit of the Carpathian flysch due to its size, stratigraphic, and tectonic complexity. Our purpose was to identify the major types of foraminifera assemblages in relation to the paleoenvironmental settings and their biostratigraphic potential. The identified assemblages are characteristic to the Cretaceous and Paleogene, consisting mostly of benthic agglutinated and, in lower proportions, benthic calcareous and planktonic species. Local abundances of Glomospira specimens allowed the correlation of the examined strata to the early Eocene “Glomospira event” described from the Carpathians in Poland, Morocco, and Labrador. Rzehakina fissistomata (Grzybowski identified at Palma makes possible the correlation of these deposits to the Paleocene Rzehakina fissistomata Zone. Paleoenvironmental conditions (depth, amount of oxygen, nutrients could be inferred based on specific assemblages and compared to the already described types of facies from the Carpathians.

  14. Multivalent nanofibers of a controlled length: regulation of bacterial cell agglutination.

    Science.gov (United States)

    Lee, Dong-Woo; Kim, Taehoon; Park, Il-Soo; Huang, Zhegang; Lee, Myongsoo

    2012-09-12

    Control of the size and shape of molecular assemblies on the nanometer scale in aqueous solutions is very important for the regulation of biological functions. Among the well-defined supramolecular structures of organic amphiphiles, one-dimensional nanofibers have attracted much attention because of their potential applications in biocompatible materials. Although much progress has been made in the field of self-assembled nanofibers, the ability to control the fiber length remains limited. The approach for control of the fiber length presented herein overcomes this limitation through the coassembly of amphiphilic rod-coil molecules in which the crystallinity of the aromatic segment can be regulated by π-π stacking interactions. The introduction of carbohydrate segments into the fiber exterior endows the nanofibers with the ability to adhere to bacterial cells. Notably, the fiber length systematically regulates the agglutination and proliferation of bacterial cells exposed to these fibers.

  15. Similarity Between Turkish & Akkadian Based on Rules of Inflective & Agglutinative Languages

    Directory of Open Access Journals (Sweden)

    Elşad Allili

    2014-08-01

    Full Text Available Akkadian, although a dead language, has left deep imprints on Semitic and some Indo-European languages, and has played an important role in the history of mankind. It is accepted as the ancestor of all the Semitic languages. Beginning from the era of Sargon I, it became the official language in a vast area from Anatolia to Egypt and to India. Akkadian was the “Lingua Franca” of the ancient world, and has passed on many words to other languages such as Persian, Sanskrit and Greek. Although, Assyriologists at present ignore it, the language spoken in the very early days of Akkad, in BCE XXVIII-XXIV, may have been an agglutinative language like today’s Turkish or Magyar, rather than an inflective language like today’s Arabic and all Syriac languages. Thus it may show parallelism with Turkish.

  16. Phoneme-level speech and natural language intergration for agglutinative languages

    CERN Document Server

    Lee, G; Kim, K; Lee, Geunbae; Lee, Jong-Hyeok; Kim, Kyunghee

    1994-01-01

    A new tightly coupled speech and natural language integration model is presented for a TDNN-based large vocabulary continuous speech recognition system. Unlike the popular n-best techniques developed for integrating mainly HMM-based speech and natural language systems in word level, which is obviously inadequate for the morphologically complex agglutinative languages, our model constructs a spoken language system based on the phoneme-level integration. The TDNN-CYK spoken language architecture is designed and implemented using the TDNN-based diphone recognition module integrated with the table-driven phonological/morphological co-analysis. Our integration model provides a seamless integration of speech and natural language for connectionist speech recognition systems especially for morphologically complex languages such as Korean. Our experiment results show that the speaker-dependent continuous Eojeol (word) recognition can be integrated with the morphological analysis with over 80\\% morphological analysis s...

  17. Performance of CHROMagar Staph aureus and CHROMagar MRSA for detection of Staphylococcus aureus in seawater and beach sand--comparison of culture, agglutination, and molecular analyses.

    Science.gov (United States)

    Goodwin, K D; Pobuda, M

    2009-11-01

    Beach seawater and sand were analyzed for Staphylococcus aureus and methicillin resistant S. aureus (MRSA) for samples collected from Avalon, and Doheny Beach, CA. Membrane filtration followed by incubation on CHROMagar Staph aureus (SCA) and CHROMagar MRSA (C-MRSA) was used to enumerate S. aureus and MRSA, respectively. Media performance was evaluated by comparing identification via colony morphology and latex agglutination tests to PCR (clfA, 16S, and mecA genes). Due to background color and crowding, picking colonies from membrane filters and streaking for isolation were sometimes necessary. The specificity of SCA and C-MRSA was improved if colony isolates were identified by the presence of a matte halo in addition to mauve color; however routine agglutination testing of isolates did not appear warranted. Using the appearance of a colony on the membrane filter in conjunction with isolate appearance, the positive % agreement, the negative % agreement, and the % positive predictive accuracy for SCA was 84%, 95%, and 99% respectively, and for C-MRSA it was 85%, 98%, and 92%, respectively. Sensitivity and specificity of SCA and C-MRSA with membrane-filtered beach samples were optimized through identification experience, control of filter volume and incubation time, and isolation of colonies needing further identification. With optimization, SCA and C-MRSA could be used for enumeration of S. aureus and MRSA from samples of beach water and sand. For the sites studied here, the frequency of detection of S. aureus ranged from 60 to 76% and 53 to 79% for samples of beach seawater and sand, respectively. The frequency of detection of MRSA ranged from 2 to 9% and 0 to 12% for samples of seawater and sand, respectively.

  18. A novel C-type lectin, Nattectin-like protein, with a wide range of bacterial agglutination activity in large yellow croaker Larimichthys crocea.

    Science.gov (United States)

    Lv, Changhuan; Zhang, Dongling; Wang, Zhiyong

    2016-03-01

    C-type lectins (CTLs) are generally recognized as a superfamily of Ca(2+)-dependent carbohydrate-binding proteins, which serve as pattern recognition receptors (PRRs) in innate immunity of vertebrates. In this study, the molecular characterization and immune roles of a novel CTL from Larimichthys crocea (designated as LcNTC) were investigated. LcNTC is a novel protein that shared 33%-49% homology with other teleosts CTLs. The full-length cDNA of LcNTC was composed of 859 bp with a 465 bp open reading frame encoding a putative protein of 154 residues. LcNTC contained a single CRD with four conserved disulfide-bonded cysteine residues (Cys(57)-Cys(148), Cys(126)-Cys(140)) and EPN/AND motifs instead of invariant EPN/WND motifs required for carbohydrate-binding specificity and constructing Ca(2+)-binding sites. LcNTC mRNA was detected in all examined tissues with the most abundant in the gill. After challenged with poly I:C and Vibrio parahaemolyticus, the temporal expression of LcNTC was significantly up-regulated in the liver, spleen and head-kidney. LcNTC transcripts were also induced in the gill, skin, spleen and head-kidney post-infection with Cryptocaryon irritans. The recombinant LcNTC (rLcNTC) purified from Escherichia coli BL21 (DE3) exhibited strong agglutination activity against erythrocytes from human, rabbit and large yellow croaker in a Ca(2+)-dependent manner, and the agglutination could be inhibited by D-Mannose, D-Glucose, D-Fructose, α-Lactose, D-Maltose and LPS. Positive microbial agglutination activities of rLcNTC were observed against all tested bacteria in the presence of Ca(2+), including Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Micrococcus lysoleikticus) and Gram-negative bacteria (E. coli, V. parahaemolyticus, Vibrio alginolyticus and Aeromonas hydrophila). These findings collectively indicated that LcNTC might be involved in the innate immunity of L. crocea as a PRR.

  19. Agglutination of Trypanosoma cruzi in infected cells treated with serum from chronically infected mice.

    Science.gov (United States)

    Wendelken, Jennifer L; Rowland, Edwin C

    2009-04-01

    The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease. The chronic stage of infection is characterized by a production of neutralizing antibodies in the vertebrate host. A polyclonal antibody, anti-egressin, has been found to inhibit egress of parasites from the host cell late in the intracellular cycle, after the parasites have transformed from the replicative amastigote into the trypomastigote. It has also been found that BALB/c mouse fibroblasts in the late stages of parasite infection become permeable to molecules as large as antibodies, leading to the possibility that anti-egressin affects the intracellular parasites. This project addresses the fate of the intracellular trypomastigotes that have been inhibited from egressing the host cell. Extended cultures of infected fibroblasts treated with chronic mouse serum reduced parasite egress at all time points measured. Parasites released from infected fibroblasts treated with chronic serum had a reduced ability to infect fibroblasts in culture, yet did not lose infectivity entirely. Absorption of chronic serum with living trypomastigotes removed the anti-egressin effect. The possibility that the target of anti-egressin is a parasite surface component is further indicated by the agglutination of extracellular trypomastigotes by chronic serum. The possibility that cross-linking by antibody occurs intracellularly, thus inhibiting egress, was reinforced by cleaving purified IgG into Fab fragments, which did not inhibit egress when added to infected cultures. From this work, it is proposed that the current, best explanation of the mechanism of egress inhibition by anti-egressin is intracellular agglutination, preventing normal parasite-driven egress.

  20. Physical, morphological and dosimetric characterization of the Teflon agglutinator to thermoluminescent dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    D' Amorim, R.A.P.O., E-mail: raquelalinepe@gmail.com [Departamento de Física-CCET, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE (Brazil); Teixeira, M.I., E-mail: miteixeira@ipen.br [Instituto de Pesquisas Energéticas e Nucleares-IPEN-CNEN/SP, Av. Professor Lineu Prestes, 2242, 05508-000 São Paulo (Brazil); Caldas, L.V.E., E-mail: lcaldas@ipen.br [Instituto de Pesquisas Energéticas e Nucleares-IPEN-CNEN/SP, Av. Professor Lineu Prestes, 2242, 05508-000 São Paulo (Brazil); Souza, S.O., E-mail: susanasouzalalic@gmail.com [Departamento de Física-CCET, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE (Brazil)

    2013-04-15

    In preparing of thermoluminescent dosimeters (TLD) it is common to use as agglutinator the polytetrafluoroethylene (PTFE), called Teflon{sup ®}. In this paper the physical, morphological and dosimetric characteristics of Teflon{sup ®} were evaluated aiming its application in thermoluminescent dosimetry. The differential thermal analysis (DTA) and thermogravimetry (TG) results showed that the Teflon glass transition and melting points are of about 48 °C and 340 °C, respectively. By means of the X-ray diffraction technique, the crystallinity index K{sub c} was estimated as 94%. Micrographs of Scanning Electron Microscopy (SEM) showed a cohesive surface in spodumene–Teflon pellets, as required for thermoluminescent dosimeters (TLD), leading to the conclusion that Teflon acts as binder, providing greater mechanical resistance to the TL pellets. However, Teflon may influence high doses dosimetry when it is applied as an agglutinator. Preliminary results of Teflon pellets dosimetric properties, with their dose–response curve between 50 Gy and 60 kGy, TL response reproducibility and minimum detectable dose, indicate the possibility of use of pure Teflon TLD in high-dose dosimetry. -- Highlights: ► Pure Teflon{sup ®} pellets can be exploited for high-dose dosimetry. ► Pure Teflon{sup ®} pellets showed two TL peaks. ► Low dose limits of Teflon{sup ®} pellets were 7.0 and 4.0 Gy for the first and second TL peaks respectively. ► The reproducibility of TL response of Teflon{sup ®} pellets is 2.9%.

  1. Modeling of Virion Collisions in Cervicovaginal Mucus Reveals Limits on Agglutination as the Protective Mechanism of Secretory Immunoglobulin A.

    Science.gov (United States)

    Chen, Alex; McKinley, Scott A; Shi, Feng; Wang, Simi; Mucha, Peter J; Harit, Dimple; Forest, M Gregory; Lai, Samuel K

    2015-01-01

    Secretory immunoglobulin A (sIgA), a dimeric antibody found in high quantities in the gastrointestinal mucosa, is broadly associated with mucosal immune protection. A distinguishing feature of sIgA is its ability to crosslink pathogens, thereby creating pathogen/sIgA aggregates that are too large to traverse the dense matrix of mucin fibers in mucus layers overlying epithelial cells and consequently reducing infectivity. Here, we use modeling to investigate this mechanism of "immune exclusion" based on sIgA-mediated agglutination, in particular the potential use of sIgA to agglutinate HIV in cervicovaginal mucus (CVM) and prevent HIV transmission. Utilizing reported data on HIV diffusion in CVM and semen, we simulate HIV collision kinetics in physiologically-thick mucus layers-a necessary first step for sIgA-induced aggregation. We find that even at the median HIV load in semen of acutely infected individuals possessing high viral titers, over 99% of HIV virions will penetrate CVM and reach the vaginal epithelium without colliding with another virion. These findings imply that agglutination is unlikely to be the dominant mechanism of sIgA-mediated protection against HIV or other sexually transmitted pathogens. Rather, we surmise that agglutination is most effective against pathogens either present at exceedingly high concentrations or that possess motility mechanisms other than Brownian diffusion that significantly enhance encounter rates.

  2. Carbon nanotube/biocompatible bola-amphiphile supramolecular biohybrid materials: preparation and their application in bacterial cell agglutination.

    Science.gov (United States)

    Yu, Guocan; Li, Jinying; Yu, Wei; Han, Chengyou; Mao, Zhengwei; Gao, Changyou; Huang, Feihe

    2013-11-26

    Supramolecular biohybrid materials were successfully constructed driven by non-covalent interactions between three biocompatible bolaform amphiphiles and single walled carbon nanotubes (SWNTs). The existence of galactoses in these supramolecular systems endowed the hybrid materials with interesting bio-function. By introducing the SWNTs as semi-flexible platforms, these supramolecular biohybrid materials display excellent agglutination ability for E. coli.

  3. Fuzzy Sliding Mode Control of Plate Vibrations

    OpenAIRE

    Manu Sharma; Singh, S. P.

    2010-01-01

    In this paper, fuzzy logic is meshed with sliding mode control, in order to control vibrations of a cantilevered plate. Test plate is instrumented with a piezoelectric sensor patch and a piezoelectric actuator patch. Finite element method is used to obtain mathematical model of the test plate. A design approach of a sliding mode controller for linear systems with mismatched time-varying uncertainties is used in this paper. It is found that chattering around the sliding surface in the sliding ...

  4. A low cost and high throughput magnetic bead-based immuno-agglutination assay in confined droplets.

    Science.gov (United States)

    Teste, Bruno; Ali-Cherif, Anaïs; Viovy, Jean Louis; Malaquin, Laurent

    2013-06-21

    Although passive immuno-agglutination assays consist of one step and simple procedures, they are usually not adapted for high throughput analyses and they require expensive and bulky equipment for quantitation steps. Here we demonstrate a low cost, multimodal and high throughput immuno-agglutination assay that relies on a combination of magnetic beads (MBs), droplets microfluidics and magnetic tweezers. Antibody coated MBs were used as a capture support in the homogeneous phase. Following the immune interaction, water in oil droplets containing MBs and analytes were generated and transported in Teflon tubing. When passing in between magnetic tweezers, the MBs contained in the droplets were magnetically confined in order to enhance the agglutination rate and kinetics. When releasing the magnetic field, the internal recirculation flows in the droplet induce shear forces that favor MBs redispersion. In the presence of the analyte, the system preserves specific interactions and MBs stay in the aggregated state while in the case of a non-specific analyte, redispersion of particles occurs. The analyte quantitation procedure relies on the MBs redispersion rate within the droplet. The influence of different parameters such as magnetic field intensity, flow rate and MBs concentration on the agglutination performances have been investigated and optimized. Although the immuno-agglutination assay described in this work may not compete with enzyme linked immunosorbent assay (ELISA) in terms of sensitivity, it offers major advantages regarding the reagents consumption (analysis is performed in sub microliter droplet) and the platform cost that yields to very cheap analyses. Moreover the fully automated analysis procedure provides reproducible analyses with throughput well above those of existing technologies. We demonstrated the detection of biotinylated phosphatase alkaline in 100 nL sample volumes with an analysis rate of 300 assays per hour and a limit of detection of 100 pM.

  5. Ultrasonic and eddy current testing of austenitic platings of reactor pressure vessels - qualification according the ENIQ method; Ultraschall- und Wirbelstrompruefung austenitischer Plattierungen von Reaktorbehaeltern - Qualifizierung nach der Methode von ENIQ

    Energy Technology Data Exchange (ETDEWEB)

    Just, T.; Csapo, G. [TUeV NORD SysTec GmbH and Co. KG, Hamburg (Germany); Brenner, W. [TUeV Sueddeutschland-ET, Mannheim (Germany); Waidele, H. [MPA, Univ. Stuttgart (Germany)

    2004-07-01

    In the context of the research project SR2318, which received funds from the Federal Radiation Protection Office (Bundesamt fuer Strahlenschutz) and the BMU, the accuracy of eddy current and ultrasonic tests of austenitic platings on reactor pressure vessels was investigated. The results were evaluated with a view to qualification of combined ultrasonic and eddy current tests of platings and base materials on the one hand; on the other hand, a standard test procedure according to the ENIQ method (European Network of Inspection Qualification) is proposed which can serve as a basis for qualification of test procedures. Summarizing suggestions are made for updating the KTA 3201.4 regulation for recurrent inspections of platings. The results of research project SR 2351 are considered. (orig.) [German] Im Rahmen des vom Bundesamt fuer Strahlenschutz bzw. des BMU gefoerderten Untersuchungsvorhabens SR2318 wurde die Aussagefaehigkeit von Ultraschall- und Wirbelstrompruefungen an austenitischen Plattierungen von Reaktordruckbehaeltern untersucht. Die im Untersuchungsvorhaben erzielten Ergebnisse werden zum einen hinsichtlich der Qualifizierung der Pruefung von Plattierungen und des daran angrenzenden Grundwerkstoffes mittels mechanisierter Ultraschallpruefung in Kombination mit der Wirbelstrompruefung bewertet, und es wird zum anderen beispielhaft ein Muster fuer eine Qualifizierung nach der Methodik von ENIQ (European Network of Inspection Qualification) vorgeschlagen, nach dem kuenftig bei Qualifikationen von Pruefverfahren vorgegangen werden kann. Als Quintessenz werden daraus Vorschlaege zur Ergaenzung der Regel KTA 3201.4 hinsichtlich der wiederkehrenden Pruefungen (WKP) von Plattierungsbereichen formuliert. Dabei sind die Ergebnisse des Untersuchungsvorhabens SR 2351 einbezogen worden. (orig.)

  6. Processless offset printing plates

    Directory of Open Access Journals (Sweden)

    Sanja Mahović Poljaček

    2015-06-01

    Full Text Available With the implementation of platesetters in the offset printing plate making process, imaging of the printing plate became more stable and ensured increase of the printing plate quality. But as the chemical processing of the printing plates still highly influences the plate making process and the graphic reproduction workflow, development of printing plates that do not require chemical processing for offset printing technique has been one of the top interests in graphic technology in the last few years. The main reason for that came from the user experience, where majority of the problems with plate making process could be connected with the chemical processing of the printing plate. Furthermore, increased environmental standards lead to reducing of the chemicals used in the industrial processes. Considering these facts, different types of offset printing plates have been introduced to the market today. This paper presents some of the processless printing plates.

  7. Standard Test Method for Measuring the Curved Beam Strength of a Fiber-Reinforced Polymer-Matrix Composite - (View Full Text) D6416/D6416M-01(2007) Standard Test Method for Two-Dimensional Flexural Properties of Simply Supported Sandwich Composite Plates Subjected to a Distributed Load

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2001-01-01

    Standard Test Method for Measuring the Curved Beam Strength of a Fiber-Reinforced Polymer-Matrix Composite - (View Full Text) D6416/D6416M-01(2007) Standard Test Method for Two-Dimensional Flexural Properties of Simply Supported Sandwich Composite Plates Subjected to a Distributed Load

  8. An Insight into the proteome of Crithidia fasciculata choanomastigotes as a comparative approach to axenic growth, peanut lectin agglutination and differentiation of Leishmania spp. promastigotes.

    Science.gov (United States)

    Alcolea, Pedro J; Alonso, Ana; García-Tabares, Francisco; Toraño, Alfredo; Larraga, Vicente

    2014-01-01

    The life cycle of the trypanosomatid Crithidia fasciculata is monogenetic, as the unique hosts of these parasites are different species of culicids. The comparison of these non-pathogenic microorganisms evolutionary close to other species of trypanosomatids that develop digenetic life cycles and cause chronic severe sickness to millions of people worldwide is of outstanding interest. A ground-breaking analysis of differential protein abundance in Crithidia fasciculata is reported herein. The comparison of the outcome with previous gene expression profiling studies developed in the related human pathogens of the genus Leishmania has revealed substantial differences between the motile stages of these closely related organisms in abundance of proteins involved in catabolism, redox homeostasis, intracellular signalling, and gene expression regulation. As L. major and L. infantum agglutinate with peanut lectin and non-agglutinating parasites are more infective, the agglutination properties were evaluated in C. fasciculata. The result is that choanomastigotes are able to agglutinate with peanut lectin and a non-agglutinating subpopulation can be also isolated. As a difference with L. infantum, the non-agglutinating subpopulation over-expresses the whole machinery for maintenance of redox homeostasis and the translation factors eIF5a, EF1α and EF2, what suggests a relationship between the lack of agglutination and a differentiation process.

  9. Evaluation of korzincalloy prepared by Hohman Plating

    Energy Technology Data Exchange (ETDEWEB)

    Korinko, P. S. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Hollingshad, A. N. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2017-07-17

    A commercial vendor, Hohman Plating performed contract engineering work to determine the feasibility of producing pin hole free KorZincAlloy bronze material used for zinc gettering. Samples were tested for Sn plating thickness, heat treatability, and chemistry prior to being subjected to a standardized zinc exposure. The samples absorbed zinc and were examined using visual and scanning electron microscopy. Hohman Plating successfully produced KZA that met the target composition, was pin hole free, and was an effective zinc getter.

  10. Histologia da pele da carpa prateada (Hypophtalmichthys molitrix e testes de resistência do couro Histology of silver-plated carp skin (Hypophtalmichthys molitrix and leather resistance tests

    Directory of Open Access Journals (Sweden)

    Maria Luiza Rodrigues de Souza

    2006-08-01

    Full Text Available Este estudo foi realizado com os objetivos de analisar a histologia da pele e avaliar a influência da técnica de curtimento e da posição da retirada do corpo-de-prova sobre a qualidade da pele de carpa prateada (Hypophtalmichthys molitrix. Vinte e três peixes foram insensibilizados para retirada das peles para curtimento. Amostras referentes a três peixes foram fixadas em formol 10%. Após a inclusão em parafina, foram cortadas com aproximadamente 5 mm de espessura e coradas pela técnica de hematoxilina-eosina (HE. Após o curtimento das peles, foram retirados os corpos-de-prova para os testes de resistência. Os testes foram realizados com dinamômetro EMIC, com velocidade de afastamento entre cargas de 100 ± 20 mm/mm, em ambiente climatizado a 23ºC e umidade relativa do ar de 50% por um período de 24 horas. As peles curtidas com sais de cromo apresentaram maior resistência à tração (16,96 N/mm², ao alongamento (51,49% e ao rasgamento progressivo (16,75 N/mm quando comparadas àquelas curtidas sem sais de cromo (tração = 9,12 N/mm², alongamento = 17,48% e rasgamento = 11,36 N/mm. O sentido da retirada dos corpos-de-prova não interferiu nas características de tração, elongação, carga de ruptura, rasgo, força máxima e carga de força. A técnica de curtimento (utilizando ou não sais de cromo influencia a resistência das peles de carpa prateada. A técnica empregando os sais de cromo proporciona maior resistência ao couro.The objectives of this trial were to analyze the skin histology and to evaluate the influence of tanning technique and the direction removal of samples on skin quality of silver-plated carp (Hypophtalmichthys molitrix. Twenty-three fishes were sacrificed and the skin was removed for tanning. Samples of three fishes were settled in formol 10% and, after inclusion in paraffin, the samples were cut with approximately 5 mm of thickness and colored by the hematoxylin-eosin technique (HE. After skin

  11. An acousto-optical method for registration of erythrocytes' agglutination reaction—sera color influence on the resolving power

    Science.gov (United States)

    Doubrovski, V. A.; Medvedeva, M. F.; Torbin, S. O.

    2016-01-01

    The absorption spectra of agglutinating sera were used to determine blood groups. It was shown experimentally that the sera color significantly affects the resolving power of the acousto-optical method of blood typing. In order to increase the resolving power of the method and produce an invariance of the method for sera color, we suggested introducing a probing light beam individually for different sera. The proposed technique not only improves the resolving power of the method, but also reduces the risk of false interpretation of the experimental results and, hence, error in determining the blood group of the sample. The latter is especially important for the typing of blood samples with weak agglutination of erythrocytes. This study can be used in the development of an instrument for instrumental human blood group typing based on the acousto-optical method.

  12. Single-Cylinder Engine Tests of Porous Chrome-Plated Cylinder Barrels with Special Bore Coatings for Radial Air-Cooled Engines

    Science.gov (United States)

    1946-01-01

    sulphamate solution at room temperature having a hydrogen-ion concentration of 1.5. The cylinder was plated for 2 minutes with a current density of 40...for any metal combination (reference 3), engine friction may be reduced somewhat by the use of a silver overplate as a bearing material on the...Cooled Engines. NACA ARR No. E5L18, 1945. 3. Hoyt, Samuel L.: Metals and Alloys Data Book. Reinhold Pub. Corp., 1943, p. 281. 4. Downing, B. F

  13. Modern agglutinated Foraminifera from the Hovgaard Ridge, Fram Strait, west of Spitzbergen: Evidence for a deep bottom current

    OpenAIRE

    Kaminski, M.A.; Niessen, Frank; Bazhenova, E.; De La Guardia, L C.; Coakley, B.; de Vernal, A.; Eagles, Graeme; Eisermann, Hannes; Forwick, Matthias; Gebhardt, Catalina; Geissler, Wolfram; Horner, T.; Jensen, Laura; Jin, H.; Jokat, W.

    2015-01-01

    Deep-water agglutinated foraminifera on the crest of the Hovgaard Ridge, west of Spitsbergen, consist mostly of large tubular astrorhizids. At a boxcore station collected from the crest of Hovgaard Ridge at a water depth of 1169 m, the sediment surface was covered with patches of large (1 mm diameter) tubular forms, be longing mostly to the species Astrorhiza crassatina Brady, with smaller numbers of Saccorhiza, Hyperammina, and Psammosiphonella. Non-tubular species consisted mainly of ...

  14. Uncertainty evaluation of aerobic plate count by proficiency testing%能力验证菌落总数测定结果不确定度的评定

    Institute of Scientific and Technical Information of China (English)

    王海华; 兰茜

    2015-01-01

    目的:对实验室能力验证样品菌落总数进行不确定度评定。方法能力验证菌落总数依据 GB 4789.2-2010《食品安全国家标准食品微生物学检验菌落总数测定》以及能力验证计划参试指导书来进行测定和结果判断,再根据JJF 1059.1-2012《测量不确定度评定与表示》的规定,对测定过程中引入的不确定度分量进行评定,然后采用合成的方法来计算和评定菌落总数的不确定度。结果菌落总数测定结果合成不确定度为0.05042,扩展不确定度为0.1008(以对数计)。结论本研究建立的方法可以对能力验证样品的菌落总数进行不确定度评定,此方法适合于类似检测条件下菌落总数不确定度的评定。%ABSTRACT:Objective To evaluate the uncertainty of aerobic plate count by proficiency testing. Methods Proficiency testing of aerobic plate count was conducted under the direction of GB 4789.2-2010 National food safety standard microbiological examination in food:Aerobic plate count and CFAPA-024 Reference book for microbiological ability verification plan in food. Then, the uncertainty components introduced in the experiment were determined according to JJF1059.1-2012 Evaluation and expression of uncertainty in measurements. Finally, synthetic method was used to calculate and assess the uncertainty of aerobic plate count. Results The combined uncertainty of aerobic plate count was 0.05042, the expanded uncertainty was U(1og)=0.1008. Conclusion The method established in this study can be used in the uncertainty evaluation of aerobic plate count under similar circumstances.

  15. Reasons Analysis on Laminated Fracture of DH36 Hull Plate During Tensile Test%DH36船板钢拉伸断口分层的原因分析

    Institute of Scientific and Technical Information of China (English)

    张鑫; 郭汉杰; 李宏亮; 吴建中; 刘锦强

    2012-01-01

    The reasons for the laminated fracture tinder tensile test of DH36 hull plate were researched by metallurgical observation and SEM observation. The results show that the main reasons are the aggregation of MnS as well as the martensite and bainite banded structure in the center of plate.%采用金相检验、扫描电镜检验等手段对DH36船板钢拉伸断口分层缺陷进行了分析.结果表明:DH36船板钢拉伸断口分层缺陷主要是由钢板厚度中心条状MnS夹杂和宽大的贝氏体、马氏体带状组织引起的.

  16. Plating on some difficult-to-plate metals and alloys

    Energy Technology Data Exchange (ETDEWEB)

    Dini, J.W.; Johnson, H.R.

    1980-02-01

    Electrodeposition of coatings on metals such as beryllium, beryllium-copper, Kovar, lead, magnesium, thorium, titanium, tungsten, uranium, zirconium, and their alloys can be problematic. This is due in most cases to a natural oxide surface film that readily reforms after being removed. The procedures we recommend for plating on these metals rely on replacing the oxide film with a displacement coating, or etching to allow mechanical keying between the substrate and plated deposit. The effectiveness of the procedures is demonstrated by interface bond strengths found in ring-shear and conical-head tensile tests.

  17. Ammolagena clavata (Jones and Parker, 1860), an agglutinated benthic foraminiferal species - first report from the Recent sediments, Arabian Sea, Indian Ocean region

    Digital Repository Service at National Institute of Oceanography (India)

    Nigam, R.; Mazumder, A.; Saraswat, R.

    The rare presence of the agglutinated foraminiferal species Ammolagena clavata is presented for the first time from the Recent sediments of the Indian Ocean region. This species has previously been reported in Recent sediments from all other oceans...

  18. Create Your Plate

    Medline Plus

    Full Text Available ... steps to get started: Using your dinner plate, put a line down the middle of the plate. ... vegetables . Now in one of the small sections, put grains and starchy foods. See this list of ...

  19. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate It's simple and effective for both managing diabetes and losing weight. Creating your plate lets ... 2016 Articles from Diabetes Forecast® magazine: wcie-meal-planning, In this section Food Planning Meals Diabetes Meal ...

  20. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook with Heart-Healthy Foods Holiday Meal ... Healthy Diet Create Your Plate Meal Planning for Vegetarian Diets Gluten Free Diets Holiday Meal Planning Cook ...

  1. Food guide plate

    Science.gov (United States)

    ... chips or cookies. VEGETABLES: MAKE HALF OF YOUR PLATE FRUITS AND VEGETABLES Vegetables can be raw, fresh, ... as a snack. FRUITS: MAKE HALF OF YOUR PLATE FRUITS AND VEGETABLES Fruits can be fresh, canned, ...

  2. Growth Plate Fractures

    Science.gov (United States)

    ... the most widely used by doctors is the Salter-Harris system, described below. Type I Fractures These ... incidence of growth plate fractures peaks in adolescence. Salter-Harris classification of growth plate fractures. AAOS does ...

  3. Create Your Plate

    Medline Plus

    Full Text Available ... Planning Meals Diabetes Meal Plans Create Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook ... Create Your Plate Meal Planning for Vegetarian Diets Gluten Free Diets Holiday Meal Planning Cook with Heart- ...

  4. Create Your Plate

    Medline Plus

    Full Text Available ... Planning Meals Diabetes Meal Plans Create Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook ... Create Your Plate Meal Planning for Vegetarian Diets Gluten Free Diets Holiday Meal Planning Cook with Heart- ...

  5. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate It's simple and effective for both managing diabetes and losing weight. Creating your plate lets you still choose the foods you want, but changes the portion sizes so you are getting larger ...

  6. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate It's simple and effective for both managing diabetes and losing weight. Creating your plate lets ... Sleeve Custom jerseys for your Tour de Cure team benefits the cause. Ask the Experts: Learn to ...

  7. Accelerated plate tectonics.

    Science.gov (United States)

    Anderson, D L

    1975-03-21

    The concept of a stressed elastic lithospheric plate riding on a viscous asthenosphere is used to calculate the recurrence interval of great earthquakes at convergent plate boundaries, the separation of decoupling and lithospheric earthquakes, and the migration pattern of large earthquakes along an arc. It is proposed that plate motions accelerate after great decoupling earthquakes and that most of the observed plate motions occur during short periods of time, separated by periods of relative quiescence.

  8. Clinical application of Brucella fast three tests in the diagnosis of human brucellosis%布病快检三项在布鲁杆菌病中的临床应用

    Institute of Scientific and Technical Information of China (English)

    刘景瑶; 赵冬梅; 毕惠梅; 何晶晶; 张柠

    2016-01-01

    Objective To explore the clinical significance of Brucella fast three tests in the diagnosis of human brucellosis.Methods Choose to our hospital brucella patients and healthy physical examination as the research object,Fluorescence polarization assay、IELISA、Rose Bengal plate agglutination test (RBPT) and standard tube agglutination test (SAT).Results Brucella fast three tests and test tube agglutination test (SAT) detection method of detecting results have good consistency (Kappa ≥ 0.75).Coincidence rate 98.3 % and sensitivity 97.2% of Brucella fast three slightly higher than tube agglutination test (SAT) method.Conclusions Brucella fast three more traditional serological method operation is simple,rapid,For clinical diagnosis and treatment of brucellosis saves time,Is worth popularizing in clinical brucellosis diagnostic applications.%目的 探讨布病快检三项在布鲁杆菌病诊断中的临床应用意义.方法 选取来黑龙江省农垦总局总医院就诊布鲁杆菌病患者和健康体检者为研究对象,分别用间接酶联免疫吸附试验(IELISA)、荧光偏振检测方法(FPA)、传统虎红试验(RBPT)和试管凝集试验(SAT)检测布鲁杆菌病.结果 布病快检三项与试管凝集试验(SAT)检测方法的检测结果均有较好的一致性(Kappa值均≥0.75).布病快检三项的符合率98.3%和灵敏度97.2%要稍高于试管凝集试验(SAT)检测方法.结论 布病快检三项较传统的血清学方法操作简单、快速,为临床布鲁杆菌病诊断和治疗节省了时间,值得在临床布鲁杆菌病诊断中推广应用.

  9. Create Your Plate

    Medline Plus

    Full Text Available ... Reset Plate Share Create Your Plate ! Share: Seven Simple Steps to Create Your Plate It's simple and effective for both managing diabetes and losing ... en.html Have Type 2 Diabetes? Our free program will help you live well. More from diabetes. ...

  10. Obliquity along plate boundaries

    Science.gov (United States)

    Philippon, Mélody; Corti, Giacomo

    2016-12-01

    Most of the plate boundaries are activated obliquely with respect to the direction of far field stresses, as roughly only 8% of the plate boundaries total length shows a very low obliquity (ranging from 0 to 10°, sub-orthogonal to the plate displacement). The obliquity along plate boundaries is controlled by (i) lateral rheological variations within the lithosphere and (ii) consistency with the global plate circuit. Indeed, plate tectonics and magmatism drive rheological changes within the lithosphere and consequently influence strain localization. Geodynamical evolution controls large-scale mantle convection and plate formation, consumption, and re-organization, thus triggering plate kinematics variations, and the adjustment and re-orientation of far field stresses. These geological processes may thus result in plate boundaries that are not perpendicular but oblique to the direction of far field stresses. This paper reviews the global patterns of obliquity along plate boundaries. Using GPlate, we provide a statistical analysis of present-day obliquity along plate boundaries. Within this framework, by comparing natural examples and geological models, we discuss deformation patterns and kinematics recorded along oblique plate boundaries.

  11. Impact behaviour of stiffened steel plates

    OpenAIRE

    Sølvernes, Sindre Hellem

    2015-01-01

    The impact behavior of stiffened steel plates subjected to impact loading from concentrated loads was studied experimentally and numerically. Both dynamic and quasi-static tests of stiffened steel plates with geometry adopted from a typical external deck area on an offshore platform were conducted. The quasi-static tests were performed to study the relationship between dynamic impact behavior and the corresponding static ones. All tests were carried out in scale 1:4. To allow the executio...

  12. The Value of Serologic Tests for Diagnosis and Follow up of Patients having Brucellosis

    Directory of Open Access Journals (Sweden)

    Nidia E. Lucero

    2007-01-01

    Full Text Available Though diagnosis of human brucellosis is accurate when the causal agent is isolated, this procedure is not always successful and the most of patients are diagnosed on the basis of rising titres of antibodies in serum. The classical tests used for detection of antibodies to S-Brucella sp., include Rose Bengal (RBT, buffered plate antigen (BPAT, serum agglutination (SAT, 2-mercapto-ethanol (2MET and complement fixation (CFT. The modern methods are based on primary binding assays of which a competitive enzyme immunoassay (CELISA and fluorescence polarization (FPA are the best developed. For antibodies to R-Brucella sp. a rapid slide agglutination (RSAT as screening and an indirect ELISA (IELISA as confirmatory tests have been reported. We have selected 23 cases of human brucellosis that were followed up over a long period, to assess which test was most effective in detecting different stages of the disease. The patients were divided into five groups: “chronic” cases; relapses; infection acquired in a laboratory; patients presumptively infected with B. canis and cases with a long history of brucellosis. The results suggest that BPAT is a practical test that reduces non specific reactions and is more sensitive than RBT. SAT detects the acute form but cross reacts with other antibodies and the diagnostic end-point titre has not been satisfactorily established; 2MET should be discontinued because of its toxicity and the scant information it can add; CFT fails to detect the acute form and is technically complicated. CELISA correlate well with the clinical course and is useful to detect acute as well as “chronic” cases and FPA do not work in serum with high lipid content. RSAT and IELISA are useful tests for brucellosis caused by B. canis. A unique protocol for serologic diagnosis that uses robust tests would be of value to the surveillance and control the disease.

  13. Adhesion, invasion, and agglutination mediated by two trimeric autotransporters in the human uropathogen Proteus mirabilis.

    Science.gov (United States)

    Alamuri, Praveen; Löwer, Martin; Hiss, Jan A; Himpsl, Stephanie D; Schneider, Gisbert; Mobley, Harry L T

    2010-11-01

    Fimbriae of the human uropathogen Proteus mirabilis are the only characterized surface proteins that contribute to its virulence by mediating adhesion and invasion of the uroepithelia. PMI2122 (AipA) and PMI2575 (TaaP) are annotated in the genome of strain HI4320 as trimeric autotransporters with "adhesin-like" and "agglutinating adhesin-like" properties, respectively. The C-terminal 62 amino acids (aa) in AipA and 76 aa in TaaP are homologous to the translocator domains of YadA from Yersinia enterocolitica and Hia from Haemophilus influenzae. Comparative protein modeling using the Hia three-dimensional structure as a template predicted that each of these domains would contain four antiparallel beta sheets and that they formed homotrimers. Recombinant AipA and TaaP were seen as ∼28 kDa and ∼78 kDa, respectively, in Escherichia coli, and each also formed high-molecular-weight homotrimers, thus supporting this model. E. coli synthesizing AipA or TaaP bound to extracellular matrix proteins with a 10- to 60-fold-higher level of affinity than the control strain. Inactivation of aipA in P. mirabilis strains significantly (P < 0.01) reduced the mutants' ability to adhere to or invade HEK293 cell monolayers, and the functions were restored upon complementation. A 51-aa-long invasin region in the AipA passenger domain was required for this function. E. coli expressing TaaP mediated autoagglutination, and a taaP mutant of P. mirabilis showed significantly (P < 0.05) more reduced aggregation than HI4320. Gly-247 in AipA and Gly-708 in TaaP were indispensable for trimerization and activity. AipA and TaaP individually offered advantages to P. mirabilis in a murine model. This is the first report characterizing trimeric autotransporters in P. mirabilis as afimbrial surface adhesins and autoagglutinins.

  14. Agglutinating secretory IgA preserves intestinal epithelial cell integrity during apical infection by Shigella flexneri.

    Science.gov (United States)

    Mathias, Amandine; Longet, Stéphanie; Corthésy, Blaise

    2013-08-01

    Shigella flexneri, by invading intestinal epithelial cells (IECs) and inducing inflammatory responses of the colonic mucosa, causes bacillary dysentery. Although M cells overlying Peyer's patches are commonly considered the primary site of entry of S. flexneri, indirect evidence suggests that bacteria can also use IECs as a portal of entry to the lamina propria. Passive delivery of secretory IgA (SIgA), the major immunoglobulin secreted at mucosal surfaces, has been shown to protect rabbits from experimental shigellosis, but no information exists as to its molecular role in maintaining luminal epithelial integrity. We have established that the interaction of virulent S. flexneri with the apical pole of a model intestinal epithelium consisting of polarized Caco-2 cell monolayers resulted in the progressive disruption of the tight junction network and actin depolymerization, eventually resulting in cell death. The lipopolysaccharide (LPS)-specific agglutinating SIgAC5 monoclonal antibody (MAb), but not monomeric IgAC5 or IgGC20 MAbs of the same specificity, achieved protective functions through combined mechanisms, including limitation of the interaction between S. flexneri and epithelial cells, maintenance of the tight junction seal, preservation of the cell morphology, reduction of NF-κB nuclear translocation, and inhibition of proinflammatory mediator secretion. Our results add to the understanding of the function of SIgA-mediated immune exclusion by identifying a mode of action whereby the formation of immune complexes translates into maintenance of the integrity of epithelial cells lining the mucosa. This novel mechanism of protection mediated by SIgA is important to extend the arsenal of effective strategies to fight against S. flexneri mucosal invasion.

  15. Angular shear plate

    Science.gov (United States)

    Ruda, Mitchell C [Tucson, AZ; Greynolds, Alan W [Tucson, AZ; Stuhlinger, Tilman W [Tucson, AZ

    2009-07-14

    One or more disc-shaped angular shear plates each include a region thereon having a thickness that varies with a nonlinear function. For the case of two such shear plates, they are positioned in a facing relationship and rotated relative to each other. Light passing through the variable thickness regions in the angular plates is refracted. By properly timing the relative rotation of the plates and by the use of an appropriate polynomial function for the thickness of the shear plate, light passing therethrough can be focused at variable positions.

  16. Shaking table test study on shear walls with concrete-filled steel tube columns and embedded steel-plate%钢管混凝土边框内藏钢板剪力墙振动台试验研究

    Institute of Scientific and Technical Information of China (English)

    曹万林; 王尧鸿; 董宏英; 张建伟

    2011-01-01

    进行了4个钢板剪力墙模型的模拟地震振动台试验,其中2个模型为钢管混凝土边框钢板剪力墙,高宽比分别为1.7和3.2;2个模型为钢管混凝土边框内藏钢板剪力墙,高宽比分别为1.7和3.2.试验中输人Taft地震动,测试了各试件在不同峰值加速度下的时程地震反应和动力特性,分析了剪力墙的破坏特征.研究表明:钢管混凝土边框内藏钢板混凝土剪力墙与钢管混凝土边框钢板剪力墙相比,地震反应明显较小,抗震性能显著提高.%Four steel-plate shear walls were tested on the shaking table. Two of the walls are steel-plate shear walls with concrete-filled steel tube columns and the other two are composite shear walls with concrete-filled steel tube columns and embedded steel-plate. The ratio of height to width is 1.7 and 3.2 respectively. The Taft seismic ground motion was input in the shaking table test. Based on the experiment, dynamic characters, dynamic responses and failure modes of the four specimens in different seismic peak accelerations are contrastively analyzed. The results show that compared with the steel-plate shear walls with concrete-filled steel tube columns, shear walls with concrete-filled steel tube columns and embedded steel-plate have the following characteristics: the dynamic responses are significantly reduced, the seismic behavior is significantly improved.

  17. Scalable DNA-Based Magnetic Nanoparticle Agglutination Assay for Bacterial Detection in Patient Samples

    DEFF Research Database (Denmark)

    Mezger, Anja; Fock, Jeppe; Antunes, Paula Soares Martins

    2015-01-01

    We demonstrate a nanoparticle-based assay for the detection of bacteria causing urinary tract infections in patient samples with a total assay time of 4 h. This time is significantly shorter than the current gold standard, plate culture, which can take several days depending on the pathogen...

  18. Instruments of RT-2 Experiment onboard CORONASPHOTON and their test and evaluation III: Coded Aperture Mask and Fresnel Zone Plates in RT-2/CZT Payload

    CERN Document Server

    Nandi, Anuj; Debnath, D; Chakrabarti, Sandip K; Kotoch, T B; Sarkar, R; Yadav, Vipin K; Girish, V; Rao, A R; Bhattacharya, D; 10.1007/s10686-010-9184-3

    2010-01-01

    Imaging in hard X-rays of any astrophysical source with high angular resolution is a challenging job. Shadow-casting technique is one of the most viable options for imaging in hard X-rays. We have used two different types of shadow-casters, namely, Coded Aperture Mask (CAM) and Fresnel Zone Plate (FZP) pair and two types of pixellated solid-state detectors, namely, CZT and CMOS in RT-2/CZT payload, the hard X-ray imaging instrument onboard the CORONAS-PHOTON satellite. In this paper, we present the results of simulations with different combinations of coders (CAM & FZP) and detectors that are employed in the RT-2/CZT payload. We discuss the possibility of detecting transient Solar flares with good angular resolution for various combinations. Simulated results are compared with laboratory experiments to verify the consistency of the designed configuration.

  19. Microstructural characterization of an irradiated RERTR-6 U-7Mo/AA4043 alloy dispersion fuel plate specimen blister-tested to a final temperature of 500 °C

    Science.gov (United States)

    Keiser, Dennis D.; Jue, Jan-Fong; Gan, Jian; Miller, Brandon D.; Robinson, Adam B.; Madden, James W.; Ross Finlay, M.; Moore, Glenn; Medvedev, Pavel; Meyer, Mitch

    2017-05-01

    The Material Management and Minimization (M3) Reactor Conversion Program, in the past called the Reduced Enrichment for Research and Test Reactor (RERTR) Program, is developing low-enriched uranium (LEU) fuels for application in research and test reactors. U-Mo alloy dispersion fuel is one type being developed. Blister testing has been performed on different fuel plate samples to determine the margin to failure for fuel plates irradiated to different fission densities. Microstructural characterization was performed using scanning electron microscopy and transmission electron microscopy on a sample taken from a U-7Mo/AA4043 matrix dispersion fuel plate irradiated in the RERTR-6 experiment that was blister-tested up to a final temperature of 500 °C. The results indicated that two types of grain/cell boundaries were observed in the U-7Mo fuel particles, one with a relatively low Mo content and fission gas bubbles and a second type enriched in Si, due to interdiffusion from the Si-containing matrix, with little evidence of fission gas bubbles. With respect to the behavior of the major fission gas Xe, a significant amount of the Xe was still observed within the U-7Mo fuel particle, along with microns into the AA4043 matrix. For the fuel/matrix interaction layers that form during fabrication and then grow during irradiation, they change from the as-irradiated amorphous structure to one that is crystalline after blister testing. In the AA4043 matrix, the original Si-rich precipitates, which are typically observed in as-irradiated U-Mo dispersion fuel, get consumed due to interdiffusion with the U-7Mo fuel particles during the blister test. Finally, the fission gas bubbles that were originally around 3 nm in diameter and resided on a fission gas superlattice (FGS) in the intragranular regions of as-irradiated U-7Mo fuel grew in size (up to ∼20 nm diameter) during blister testing and, in many areas, are no longer organized as a superlattice.

  20. A Constitutively Mannose-Sensitive Agglutinating Salmonella enterica subsp. enterica Serovar Typhimurium Strain, Carrying a Transposon in the Fimbrial Usher Gene stbC, Exhibits Multidrug Resistance and Flagellated Phenotypes

    Directory of Open Access Journals (Sweden)

    Kuan-Hsun Wu

    2012-01-01

    Full Text Available Static broth culture favors Salmonella enterica subsp. enterica serovar Typhimurium to produce type 1 fimbriae, while solid agar inhibits its expression. A transposon inserted in stbC, which would encode an usher for Stb fimbriae of a non-flagellar Salmonella enterica subsp. enterica serovar Typhimurium LB5010 strain, conferred it to agglutinate yeast cells on both cultures. RT-PCR revealed that the expression of the fimbrial subunit gene fimA, and fimZ, a regulatory gene of fimA, were both increased in the stbC mutant when grown on LB agar; fimW, a repressor gene of fimA, exhibited lower expression. Flagella were observed in the stbC mutant and this phenotype was correlated with the motile phenotype. Microarray data and RT-PCR indicated that the expression of three genes, motA, motB, and cheM, was enhanced in the stbC mutant. The stbC mutant was resistant to several antibiotics, consistent with the finding that expression of yhcQ and ramA was enhanced. A complementation test revealed that transforming a recombinant plasmid possessing the stbC restored the mannose-sensitive agglutination phenotype to the stbC mutant much as that in the parental Salmonella enterica subsp. enterica serovar Typhimurium LB5010 strain, indicating the possibility of an interplay of different fimbrial systems in coordinating their expression.

  1. Scour Protection for Monopile by Concrete Plate

    DEFF Research Database (Denmark)

    Gilling, Lasse; Skúlason, Magnús

    A test programme has been performed to determine the scour around a monopile for an offshore wind turbine. The foundation is protected against scour with a concrete plate with various designs. The tests should be regarded as pilot tests previous to a detailed investigation. The tests has been...

  2. A new type of pseudothrombocytopenia: EDTA-mediated agglutination of platelets bearing Fab fragments of a chimaeric antibody.

    Science.gov (United States)

    Christopoulos, C G; Machin, S J

    1994-07-01

    In vitro agglutination of platelets leading to low automated platelet counts was observed in EDTA-anticoagulated blood from human volunteers receiving infusions of Fab fragments of a chimaeric monoclonal antibody to platelet glycoprotein IIb-IIIa. This pseudothrombocytopenia depended on the presence of chimaeric Fab on the platelet surface and was not seen when sodium citrate was used as anticoagulent. Preliminary evidence suggests that this phenomenon might be mediated by immunoglobulin G reactive with the human component of the chimaeric Fab. It is important to exclude pseudothrombocytopenia when low automated platelet counts are reported in association with the administration of chimaeric anti-platelet antibodies.

  3. Generalized Fibonacci zone plates

    CERN Document Server

    Ke, Jie; Zhu, Jianqiang

    2015-01-01

    We propose a family of zone plates which are produced by the generalized Fibonacci sequences and their axial focusing properties are analyzed in detail. Compared with traditional Fresnel zone plates, the generalized Fibonacci zone plates present two axial foci with equal intensity. Besides, we propose an approach to adjust the axial locations of the two foci by means of different optical path difference, and further give the deterministic ratio of the two focal distances which attributes to their own generalized Fibonacci sequences. The generalized Fibonacci zone plates may allow for new applications in micro and nanophotonics.

  4. Influence of plate-bone contact on cyclically loaded conically coupled locking plate failure.

    Science.gov (United States)

    Rotne, Randi; Bertollo, Nicky; Walsh, William; Dhand, Navneet K; Voss, Katja; Johnson, Kenneth A

    2014-03-01

    The maintenance of friction between locking plates and bone is not essential, so that they can be applied with a gap between the plate and underlying bone. We hypothesised that the presence of a gap under a locking plate with a conical coupling mechanism would reduce fixation stability or allow uncoupling of the locking screws from the plate. Locking plates with two conically coupled locking screws were applied to 6 pairs of adult canine femora. One of each pair had plate to bone contact and the contralateral construct had a 2 mm plate to bone gap. Constructs were cyclically loaded in cantilever bending with 10 percent incremental increases every 1000 cycles at 2 Hz, starting at 250 N. The constructs were fatigued to failure. To evaluate fatigue life of the conical coupling, testing was repeated with aluminium tubing replacing the bone, to eliminate screw-bone cutout failure. The mean sustained loads and cycles to failure in the contact group (420.80, standard error [SE] 14.97 N; 7612.00, SE 574.70 cycles) were significantly greater than in the gap group (337.50, SE 14.97 N; 4252.00, SE 574.70 cycles), (pplate fatigue and breaking, with one construct having elevation of the plate over the screw head. Elevation of locking plates with a conical coupling system by 2 mm from the bone reduced construct fatigue life but did not result in screw head uncoupling from the plate. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Construction of supported lipid membrane modified piezoelectric biosensor for sensitive assay of cholera toxin based on surface-agglutination of ganglioside-bearing liposomes.

    Science.gov (United States)

    Chen, Huan; Hu, Qing-Yuan; Yue-Zheng; Jiang, Jian-Hui; Shen, Guo-Li; Yu, Ru-Qin

    2010-01-11

    A novel piezoelelctric biosensor has been developed for cholera toxin (CT) detection based on the analyte-mediated surface-agglutination of ganglioside (GM1)-functionalized liposomes. To achieve a CT-specific agglutination at the surface, the gold electrode is modified by a GM1-functionalized supported lipid membrane via spontaneous spread of the liposomes on a self-assembled monolayer of a long-chain alkanethiol. In the presence of CT, the GM1-incorporated liposomes in assay medium will rapidly specifically agglutinate at the electrode surface through the binding of CT to GM1 on the electrode surface and the liposome interface. This results in an enormous mass loading on the piezoelelctric crystal as well as a significant increase of density and viscosity at the interface, thereby generating a decrease in frequency of the piezoelelctric crystal. The combination of mass loading with interfacial change in the surface-agglutination reaction allows the developed piezoelelctric biosensor to show substantial signal amplification in response to the analyte CT. The detection limit can be achieved as low as 25 ng mL(-1) CT. This is the first demonstration on CT detection based on specific surface-agglutination of GM1-modified liposomes. The supported lipid layer based sensing interface can be prepared readily and renewably, making the developed technique especially useful for simple, reusable and sensitive determination of proteins.

  6. A NEW QUADRILATERAL THIN PLATE ELEMENT BASED ON THE MEMBRANE-PLATE SIMILARITY THEORY

    Institute of Scientific and Technical Information of China (English)

    黄若煜; 郑长良; 钟万勰; 姚伟岸

    2002-01-01

    A new effective path has been proposed to formulate thin plate element by using the similarity theory between plane elasticity and plate bending. Because of avoiding the difficulty of c1 continuity , the construction of thin plate elements becomes easier. The similarity theory and its applications were discussed more deeply, and a new four nodes, sixteen D. O. F. ( degree of fieedom) thin plate element was presented on the base of the similarity theory. Numerical results for typical problems show that this new element can pass the patch test and has a very good convergence and a high precision.

  7. Finite element analysis of elasto-plastic plate bending problems using transition rectangular plate elements

    Institute of Scientific and Technical Information of China (English)

    Bahattin Kanber; O.Yavuz Bozkurt

    2006-01-01

    In this work,the finite element analysis of the elasto-plastic plate bending problems is carried out using transition rectangular plate elements.The shape functions of the transition plate elements are derived based on a practical rule.The transition plate elements are all quadrilateral and can be used to obtain efficient finite element models using minimum number of elements.The mesh convergence rates of the models including the transition elements are compared with the regular element models.To verify the developed elements,simple tests are demonstrated and various elasto-plastic problems are solved.Their results are compared with ANSYS results.

  8. Biomechanical Study of the Fixation Strength of Anteromedial Plating for Humeral Shaft Fractures

    Directory of Open Access Journals (Sweden)

    Yin-Feng Zheng

    2016-01-01

    Conclusions: Anteromedial plating was superior to anterolateral or posterior plating in all mechanical tests except in AP four-point bending fatigue tests compared to the anterolateral plating group. We can suggest that anteromedial plating is a clinically safe and effective way for humeral shaft fractures.

  9. Blue Willow Story Plates

    Science.gov (United States)

    Fontes, Kris

    2009-01-01

    In the December 1997 issue of "SchoolArts" is a lesson titled "Blue Willow Story Plates" by Susan Striker. In this article, the author shares how she used this lesson with her middle-school students many times over the years. Here, she describes a Blue Willow plate painting project that her students made.

  10. Create Your Plate

    Medline Plus

    Full Text Available ... In Memory In Honor Become a Member En Español Type 1 Type 2 About Us Online Community ... Page Text Size: A A A Listen En Español Create Your Plate Create Your Plate is a ...

  11. Create Your Plate

    Medline Plus

    Full Text Available ... Diabetes Meal Plans Create Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook with Heart-Healthy Foods Holiday Meal Planning ... Planning Meals Diabetes Meal Plans and a Healthy Diet Create Your Plate Meal Planning for Vegetarian Diets ...

  12. Induction of Staphylococcus aureus-specific IgA and agglutination potency in milk of cows by mucosal immunization.

    Science.gov (United States)

    Tempelmans Plat-Sinnige, Marjan J; Verkaik, Nelianne J; van Wamel, Willem J B; de Groot, Nanda; Acton, Dennis S; van Belkum, Alex

    2009-06-19

    Lactating cows were immunized with inactivated Staphylococcus aureus strains and concentrated culture supernatants. Application of a repeated mucosal immunization scheme resulted in significant levels of S. aureus-specific IgA in milk of dairy cows. Average IgA titers against whole cell S. aureus increased during the first 10 weeks of immunization after which a plateau level was reached and maintained during lactation. Immune whey agglutinated both bovine and human S. aureus strains including methicillin-resistant S. aureus (MRSA) strains and recognized extracted S. aureus proteins on Western blot. ELISAs to quantify milk IgA reactive with a number of S. aureus virulence proteins (e.g. enterotoxins, microbial surface component recognizing adhesive matrix molecules (MSCRAMMs) and immune modulating proteins) and cell wall components, demonstrated the polyclonality of the IgA. Correlations observed between agglutination and specific IgA titers for whey and for purified IgA suggested functionality of the induced antibodies. Milk from immunized cows may provide a way of producing potentially therapeutic polyclonal antibodies against S. aureus colonization and infection.

  13. Insights into the Antimicrobial Mechanism of Action of Human RNase6: Structural Determinants for Bacterial Cell Agglutination and Membrane Permeation.

    Science.gov (United States)

    Pulido, David; Arranz-Trullén, Javier; Prats-Ejarque, Guillem; Velázquez, Diego; Torrent, Marc; Moussaoui, Mohammed; Boix, Ester

    2016-04-13

    Human Ribonuclease 6 is a secreted protein belonging to the ribonuclease A (RNaseA) superfamily, a vertebrate specific family suggested to arise with an ancestral host defense role. Tissue distribution analysis revealed its expression in innate cell types, showing abundance in monocytes and neutrophils. Recent evidence of induction of the protein expression by bacterial infection suggested an antipathogen function in vivo. In our laboratory, the antimicrobial properties of the protein have been evaluated against Gram-negative and Gram-positive species and its mechanism of action was characterized using a membrane model. Interestingly, our results indicate that RNase6, as previously reported for RNase3, is able to specifically agglutinate Gram-negative bacteria as a main trait of its antimicrobial activity. Moreover, a side by side comparative analysis with the RN6(1-45) derived peptide highlights that the antimicrobial activity is mostly retained at the protein N-terminus. Further work by site directed mutagenesis and structural analysis has identified two residues involved in the protein antimicrobial action (Trp1 and Ile13) that are essential for the cell agglutination properties. This is the first structure-functional characterization of RNase6 antimicrobial properties, supporting its contribution to the infection focus clearance.

  14. Monounsaturated fatty acid ether oligomers formed during heating of virgin olive oil show agglutination activity against human red blood cells.

    Science.gov (United States)

    Patrikios, Ioannis S; Mavromoustakos, Thomas M

    2014-01-29

    The present work focuses on the characterization of molecules formed when virgin olive oil is heated at 130 °C for 24 h open in air, which are found to be strong agglutinins. The hemagglutinating activity of the newly formed molecule isolated from the heated virgin olive oil sample was estimated against human red blood cells (RBCs). Dimers and polymers (high molecular weight molecules) were identified through thin layer chromatography (TLC) of the oil mixture. (1)H and (13)C nuclear magnetic resonance (NMR) and gas chromatography-mass spectroscopy (GC-MS) were the methods used for structural characterization. Among others, oligomerization of at least two monounsaturated fatty acids (FA) by an ether linkage between the hydrocarbon chains is involved. Light microscopy was used to characterize and visualize the agglutination process. Agglutination without fusion or lysis was observed. It was concluded that the heating of virgin olive oil open in air, among other effects, produces oligomerization as well as polymerization of unsaturated FA, possibly of monohydroxy, monounsaturated FA that is associated with strong hemagglutinating activity against human RBCs. The nutritional value and the effects on human health of such oligomers are not discussed in the literature and remain to be investigated.

  15. siRNA delivery targeting to the lung via agglutination-induced accumulation and clearance of cationic tetraamino fullerene

    Science.gov (United States)

    Minami, Kosuke; Okamoto, Koji; Doi, Kent; Harano, Koji; Noiri, Eisei; Nakamura, Eiichi

    2014-05-01

    The efficient treatment of lung diseases requires lung-selective delivery of agents to the lung. However, lung-selective delivery is difficult because the accumulation of micrometer-sized carriers in the lung often induces inflammation and embolization-related toxicity. Here we demonstrate a lung-selective delivery system of small interfering RNA (siRNA) by controlling the size of carrier vehicle in blood vessels. The carrier is made of tetra(piperazino)fullerene epoxide (TPFE), a water-soluble cationic tetraamino fullerene. TPFE and siRNA form sub-micrometer-sized complexes in buffered solution and these complexes agglutinate further with plasma proteins in the bloodstream to form micrometer-sized particles. The agglutinate rapidly clogs the lung capillaries, releases the siRNA into lung cells to silence expression of target genes, and is then cleared rapidly from the lung after siRNA delivery. We applied our delivery system to an animal model of sepsis, indicating the potential of TPFE-based siRNA delivery for clinical applications.

  16. Insights into the Antimicrobial Mechanism of Action of Human RNase6: Structural Determinants for Bacterial Cell Agglutination and Membrane Permeation

    Science.gov (United States)

    Pulido, David; Arranz-Trullén, Javier; Prats-Ejarque, Guillem; Velázquez, Diego; Torrent, Marc; Moussaoui, Mohammed; Boix, Ester

    2016-01-01

    Human Ribonuclease 6 is a secreted protein belonging to the ribonuclease A (RNaseA) superfamily, a vertebrate specific family suggested to arise with an ancestral host defense role. Tissue distribution analysis revealed its expression in innate cell types, showing abundance in monocytes and neutrophils. Recent evidence of induction of the protein expression by bacterial infection suggested an antipathogen function in vivo. In our laboratory, the antimicrobial properties of the protein have been evaluated against Gram-negative and Gram-positive species and its mechanism of action was characterized using a membrane model. Interestingly, our results indicate that RNase6, as previously reported for RNase3, is able to specifically agglutinate Gram-negative bacteria as a main trait of its antimicrobial activity. Moreover, a side by side comparative analysis with the RN6(1–45) derived peptide highlights that the antimicrobial activity is mostly retained at the protein N-terminus. Further work by site directed mutagenesis and structural analysis has identified two residues involved in the protein antimicrobial action (Trp1 and Ile13) that are essential for the cell agglutination properties. This is the first structure-functional characterization of RNase6 antimicrobial properties, supporting its contribution to the infection focus clearance. PMID:27089320

  17. Study of polycation effects on erythrocyte agglutination mediated by anti-glycophorins using microscopic image digital analysis

    Science.gov (United States)

    Riquelme, B.; Dumas, D.; Relancio, F.; Fontana, A.; Alessi, A.; Foresto, P.; Grandfils, C.; Stoltz, J.; Valverde, J.

    2006-04-01

    The aim of this work was to study synthetic polycation effects on erythrocyte agglutination mediated by anti-glycophorin using image digital analysis. Polycations are oligomers or polymers of natural or synthetic origin, which bear a great number of positive charges at pH 7.4. Several of these polycations are nowadays used in clinic for human and veterinary purposes. New applications of polycations to the development of new drug delivery systems are investigated, in order to promote the drug absorption through the gastro-intestinal and blood brain barriers. However, up to now, there are no clear relationships between macromolecular features of polycations (molecular weight, mean charge density, charge repartition, etc.) and their interactions with blood elements (which bear superficial negative charges). The interaction on the red blood cell membrane with synthetic polycations having well-controlled macromolecular features and functionalized with pendent polyethylene glycol segments was investigated. The alterations over stationary and dynamic viscoelastic properties of erythrocyte membranes were analyzed through laser diffractometry. Image digital analysis was used to study erythrocyte agglutination mediated by anti-glycophorin. Results show different reactivities of the polycations on the erythrocyte membrane. These findings could provide more information about the mechanisms of polycation interaction on erythrocyte membranes. We consider that this work could provide useful tools to understand and improve the haemocompatibility of polycations and enlarge their potential in clinic.

  18. Coexistence of erythrocyte agglutination and EDTA-dependent platelet clumping in a patient with thymoma and plasmocytoma.

    Science.gov (United States)

    Bizzaro, N; Fiorin, F

    1999-02-01

    For 8 years, EDTA-dependent pseudothrombocytopenia was observed in a 55-year-old woman with a history of rheumatoid arthritis who had undergone surgery for lymphoepithelial thymoma 11 years earlier. The clinical picture was characterized by the presence of platelet clumps and antiplatelet antibodies of the IgM class. With the recent appearance of a solitary extramedullary plasmocytoma in the right retrobulbar region and the detection of an IgGlambda monoclonal gammopathy, blood examination also revealed erythrocyte agglutinates alongside the platelet clumps and the presence of a cold IgG antibody with antiI specificity. Both phenomena were observed in vitro when the sample temperature declined to 20 degrees C to 25 degrees C, but not at 37 degrees C. While the EDTA-dependent antiplatelet antibodies did not appear to be chronologically correlated with the patient's diseases, the cold antierythrocyte autoantibodies were strictly related to the plasmocytoma and the IgGlambda monoclonal component in serum. To our knowledge, this is the first description of an association between EDTA-dependent platelet and erythrocyte agglutinates, with a clinical picture of pseudothrombocytopenia and pseudoerythrocytopenia due to cold agglutinins.

  19. Jacalin domain in wheat jasmonate-regulated protein Ta-JA1 confers agglutinating activity and pathogen resistance.

    Science.gov (United States)

    Ma, Qing-Hu; Zhen, Wei-Bo; Liu, Yun-Chao

    2013-02-01

    Ta-JA1 is a jacalin-like lectin from wheat (Triticum aestivum) plants. To date, its homologs are only observed in the Gramineae family. Our previous experiments have demonstrated that Ta-JA1 contains a modular structure consisting of an N-terminal dirigent domain and a C-terminal jacalin-related lectin domain (JRL) and this protein exhibits a mannose-specific lectin activity. The over-expression of Ta-JA1 gene provides transgenic plants a broad-spectrum resistance to diseases. Here, we report the differential activities of the dirigent and JRL domains of Ta-JA1. In vitro assay showed that the recombinant JRL domain could effectively agglutinate rabbit erythrocytes and pathogen bacteria Pseudomonas syringe pv tabaci. These hemagglutination activities could be inhibited by mannose but not by galactose. In contrast, the recombinant dirigent domain did not show agglutination activity. Corresponding to these differentiations of activities, similar to full-length of Ta-JA1, the over-expression of JRL domain in transgenic plants also increased resistance to the infection of P. syringe. Unlike JRL, the over-expression of dirigent domain in transgenic plants led to alteration of the seedling sensitivity to salts. In addition, a d(N)/d(S) ratio analysis of Ta-JA1 and its related proteins showed that this protein family functionally limited to a few crop plants, such as maize, rice and wheat.

  20. Correlation Between the Mobility of Inner Plasma Membrane Structure and Agglutination by Concanavalin A in Two Cell Lines of MOPC 173 Plasmocytoma Cells

    Science.gov (United States)

    Guérin, Claudine; Zachowski, Alain; Prigent, Bernadette; Paraf, Alain; Dunia, Irène; Diawara, Marie-Aline; Benedetti, E. L.

    1974-01-01

    Both the distribution of the concanavalin A-binding sites and the rearrangement of the intramembranous particles revealed by the freeze-etching technique, have been studied by means of two variants of the same cell line issued from MOPC 173 murine plasmocytoma. One variant does not agglutinate even in presence of high lectin concentration. It has been shown that the number of binding sites and affinity are almost the same in the two variants. The clustered distribution of intramembranous particles is induced by the interaction of the concanavalin A and the cell surface only in the variant which is agglutinable. From these results it became apparent that the clustered distribution of the membrane particulate components is an acquired feature of the plasma membrane accompanying cell agglutination. Images PMID:4521044

  1. Lab-on-a-disc agglutination assay for protein detection by optomagnetic readout and optical imaging using nano- and micro-sized magnetic beads

    DEFF Research Database (Denmark)

    Uddin, Rokon; Burger, Robert; Donolato, Marco;

    2016-01-01

    We present a biosensing platform for the detection of proteins based on agglutination of aptamer coated magnetic nano- or microbeads. The assay, from sample to answer, is integrated on an automated, low-cost microfluidic disc platform. This ensures fast and reliable results due to a minimum...... of manual steps involved. The detection of the target protein was achieved in two ways: (1) optomagnetic readout using magnetic nanobeads (MNBs); (2) optical imaging using magnetic microbeads (MMBs). The optomagnetic readout of agglutination is based on optical measurement of the dynamics of MNB aggregates...... whereas the imaging method is based on direct visualization and quantification of the average size of MMB aggregates. By enhancing magnetic particle agglutination via application of strong magnetic field pulses, we obtained identical limits of detection of 25 pM with the same sample-to-answer time (15 min...

  2. Ultrasonic Lamb Wave Testing of Hydrogen Bubble in Pressure Vessel Plate%压力容器板材中氢鼓泡缺陷的超声Lamb波检测

    Institute of Scientific and Technical Information of China (English)

    杨齐; 陈定岳; 陈虎; 王杜

    2016-01-01

    采用 WAVE 2000数值计算软件对一16 mm 厚人工模拟试板的内壁鼓包缺陷进行超声Lamb 波仿真模拟,模拟结果表明,采用Lamb 波从外壁对压力容器板材内壁氢鼓泡检测是可行的;同时,给出了最优检测参数,为实际检测提供了 Lamb 波模式、频率、入射角度等参数的选择参考。根据仿真结果,采用最优检测参数对人工模拟试板进行 Lamb 波的实际检测试验,结果表明:该方法检测效果良好,不仅能够检出缺陷,而且缺陷定位精度也较高。%WAVE 2000 numerical software was used for ultrasonic Lamb wave simulation of the inner wall bubble defect of a 1 6 mm thick artificial test plate.The results showed that it was feasible to detect the hydrogen bubbles in the inner wall of pressure vessel plate by using Lamb wave from the outer wall,and the optimal detection parameters were given,the reference of important parameters such as Lamb wave mode,frequency,incidence angle for actual testing was provided.According to the simulation results,the optimal detection parameters were used for Lamb wave testing,which was not only able to detect defect,but also had high localization accuracy.

  3. ANFIS Modelling of Flexible Plate Structure

    Directory of Open Access Journals (Sweden)

    A. A. M. Al-Khafaji

    2010-06-01

    Full Text Available This paper presented an investigation into the performance of system identification using an Adaptive Neuro-Fuzzy Inference System (ANFIS technique for the dynamic modelling of a twodimensional flexible plate structure. It is confirmed experimentally, using National Instrumentation (NI Data Acquisition System (DAQ and flexible plate test rig that ANFIS can be effectively used for modelling the system with highly accurate results. The accuracy of the modelling results is demonstrated through validation tests including training and test validation and correlation tests.

  4. Influence Analysis of Red Cell Cold Agglutination on the Detection Results of Different Types of Blood Cell Analyzer%红细胞冷凝集对不同类型血细胞分析仪检测结果的影响分析

    Institute of Scientific and Technical Information of China (English)

    陈燕; 许立新

    2015-01-01

    water bath,WBC,HCT and MCHC index of the red cell cold agglutination specimens when using Sysmex-1800 equipment compared with BC-5300 equipment,there were statistically significant differences(P0.05).Conclusion:The red cell cold agglutination appear a certain degree of influence on the detection results of different types of blood cell analyzer,the red blood cell cold agglutination specimens in 37 degrees celsius water bath can be tested again after eliminating cold agglutination.

  5. Detection methods of aerobic plate count on food microbiological proficiency testing%食品微生物能力验证样品菌落总数检验方法

    Institute of Scientific and Technical Information of China (English)

    肖剑; 李慧琴; 陈楷; 梁美丹; 林秀敏

    2014-01-01

    目的:探讨菌落总数能力验证的检验方法及注意事项。方法采用PCA直接倾注法、增加覆盖一层琼脂培养基的方法、添加 TTC 方法、陶瓦盖代替平皿盖的方法同时进行试验,并采用提前观察、增加观察的频率和培养时间,加强计数准确性。结果在前期培养过程中加强观察能够有助于计数,而延迟培养时间则可避免迟缓生长的细菌漏检;采用含有0.5%TTC 培养基进行培养或陶瓦盖平板法能有效抑制蔓延菌生长,且不影响总体计数结果。结论采用多种检验方法有助于菌落总数能力验证试验结果的准确报告。%ObjectiveTodiscuss the methods and precaution of aerobic plate count proficiency testing. MethodsFour methods were tested including pouring plate count agar directly, covering a layer of agar me-dium, adding TTC and using terracotta cover instead of petri dish. Meanwhile, using the early observation, in-creasing the frequency of observation and the time of cultivation to strengthen count accuracy.Results The results showed that the accuracy of results during the pre-cultivation counting was improved, and the missing of slow growing bacteria extending the time of cultivation was also avoided. In addition, it was effective to inhibit the spread of bacteria by culturing with the medium containing 0.5%TTC or using terracotta cover, and these methods did not affect the overall count results.Conclusion It is conductive to get accurate results by variety of methods for aerobic plate count proficiency testing.

  6. Aeroheating model advancements featuring electroless metallic plating

    Science.gov (United States)

    Stalmach, C. J., Jr.; Goodrich, W. D.

    1976-01-01

    Discussed are advancements in wind tunnel model construction methods and hypersonic test data demonstrating the methods. The general objective was to develop model fabrication methods for improved heat transfer measuring capability at less model cost. A plated slab model approach was evaluated with cast models containing constantan wires that formed single-wire-to-plate surface thermocouple junctions with a seamless skin of electroless nickel alloy. The surface of a space shuttle orbiter model was selectively plated with scaled tiles to simulate, with high fidelity, the probable misalignments of the heatshield tiles on a flight vehicle. Initial, Mach 8 heating results indicated a minor effect of tile misalignment roughness on boundary layer transition, implying a possible relaxation of heatshield manufacturing tolerances. Some loss of the plated tiles was experienced when the model was tested at high heating rates.

  7. Surface structure biomimetic design and performance testing of slippery trapping plate used for controlling agricultural insect%致灾农业昆虫捕集滑板表面结构仿生构建与性能验证

    Institute of Scientific and Technical Information of China (English)

    王立新; 黄风山; 周强

    2015-01-01

    (16.0-18.0 KV) for 100-120 s. With the incorporation of the high voltage electrostatic, the flaky graphite was absorbed to the blind holes of substrate and attached tightly. To test the function of the biomimetic slippery trapping plate, attachment forces of adult locust (Locusta migratoria manilensis) were measured with an insect micro-force measurement system in July 2015. The system mainly consisted of a force transducer (1-PW4C3), a signal conditioning module (SCXI-1520), a data acquisition platform (PCI-6221), and data-processing & displaying software. The locust was connected to the force transducer (along load direction) using a thin thread (12 cm long) fastened its neck position, and then put on the tested surface. The locust climbed on the tested surface along the load direction of the force transducer. When the thin thread started to pull, the locust crawled ahead frantically to attempt to break away from this restriction, so generated attachment forces and their maximal values were recorded. The results showed that values of attachment force provided by locust on bionic slippery trapping plate ranged from 328.7 mN to 458.3 mN, whereas on slippery surface ranged from 307.3 mN to 397.1 mN. Attachment force of locust on bionic slippery trapping plate (402.9±26.1 mN) was barely 1.1 times than that on slippery surface (361.9±25.5 mN), suggesting the biomimetic slippery trapping plate bore rather similar function as the slippery surface, thereby achieved the protected biomimetic results. The obtained conclusion provides theoretical and technical references to biomimetic development of slippery trapping plate used for controlling agricultural insect.

  8. M-cholinoreactivity of erythrocytes of non-pregnant and pregnant women evaluated by changes in the rate of erythrocyte agglutination under the influence of acetylcholine.

    Science.gov (United States)

    Strelnikova, A I; Tsirkin, V I; Krysova, A V; Hlybova, S V; Dmitrieva, S L

    2012-12-01

    Acetylcholine (5.5×10(-10)-5.5×10(-6)M) accelerated erythrocyte agglutination in men, non-pregnant women in follicular phase of the menstrual cycle, and pregnant women in the first trimester. The effect was blocked with atropine (5.5×10(-6)M). Acetylcholine had no effect on the rate of erythrocyte agglutination in non-pregnant women in the luteal phase and pregnant women in the second and third trimesters, which coincided with the development of myometrium refractoriness to acetylcholine in pregnant women. The results indicate that erythrocytes can reflect M-cholinoreactivity of internal organs.

  9. Pixelated neutron image plates

    Science.gov (United States)

    Schlapp, M.; Conrad, H.; von Seggern, H.

    2004-09-01

    Neutron image plates (NIPs) have found widespread application as neutron detectors for single-crystal and powder diffraction, small-angle scattering and tomography. After neutron exposure, the image plate can be read out by scanning with a laser. Commercially available NIPs consist of a powder mixture of BaFBr : Eu2+ and Gd2O3 dispersed in a polymer matrix and supported by a flexible polymer sheet. Since BaFBr : Eu2+ is an excellent x-ray storage phosphor, these NIPs are particularly sensitive to ggr-radiation, which is always present as a background radiation in neutron experiments. In this work we present results on NIPs consisting of KCl : Eu2+ and LiF that were fabricated into ceramic image plates in which the alkali halides act as a self-supporting matrix without the necessity for using a polymeric binder. An advantage of this type of NIP is the significantly reduced ggr-sensitivity. However, the much lower neutron absorption cross section of LiF compared with Gd2O3 demands a thicker image plate for obtaining comparable neutron absorption. The greater thickness of the NIP inevitably leads to a loss in spatial resolution of the image plate. However, this reduction in resolution can be restricted by a novel image plate concept in which a ceramic structure with square cells (referred to as a 'honeycomb') is embedded in the NIP, resulting in a pixelated image plate. In such a NIP the read-out light is confined to the particular illuminated pixel, decoupling the spatial resolution from the optical properties of the image plate material and morphology. In this work, a comparison of experimentally determined and simulated spatial resolutions of pixelated and unstructured image plates for a fixed read-out laser intensity is presented, as well as simulations of the properties of these NIPs at higher laser powers.

  10. Plate removal following orthognathic surgery.

    Science.gov (United States)

    Little, Mhairi; Langford, Richard Julian; Bhanji, Adam; Farr, David

    2015-11-01

    The objectives of this study are to determine the removal rates of orthognathic plates used during orthognathic surgery at James Cook University Hospital and describe the reasons for plate removal. 202 consecutive orthognathic cases were identified between July 2004 and July 2012. Demographics and procedure details were collected for these patients. Patients from this group who returned to theatre for plate removal between July 2004 and November 2012 were identified and their notes were analysed for data including reason for plate removal, age, smoking status, sex and time to plate removal. 3.2% of plates were removed with proportionally more plates removed from the mandible than the maxilla. 10.4% of patients required removal of one or more plate. Most plates were removed within the first post-operative year. The commonest reasons for plate removal were plate exposure and infection. The plate removal rates in our study are comparable to those seen in the literature.

  11. Anisotropic elastic plates

    CERN Document Server

    Hwu, Chyanbin

    2010-01-01

    As structural elements, anisotropic elastic plates find wide applications in modern technology. The plates here are considered to be subjected to not only in plane load but also transverse load. In other words, both plane and plate bending problems as well as the stretching-bending coupling problems are all explained in this book. In addition to the introduction of the theory of anisotropic elasticity, several important subjects have are discussed in this book such as interfaces, cracks, holes, inclusions, contact problems, piezoelectric materials, thermoelastic problems and boundary element a

  12. Plating in Top Agar

    OpenAIRE

    sprotocols

    2014-01-01

    1. Warm plates to room temperature before use. Cold plates causes the top agar to solidify irregularly. DO not warm plates to 37° as the top agar will take forever to solidify. - Prepare top agar as the appropriate liquid medium with 0.7% agar. Keeping 100 mL bottles is convenient. For phages, use λ top agar, which is less rich and yields bigger plaques. - Melt top agar in the microwave completely. Allow the agar to boil after liquification; incompletely melted agar looks liquid, but is...

  13. Therminol VP-1导热油平板太阳能集热器的热工性能测试实验%Therminol VP-1 Heat Transfer Fluid Plate Solar Collector Thermal Performance Test

    Institute of Scientific and Technical Information of China (English)

    唐千喻; 王华; 王辉涛; 卿山

    2011-01-01

    Due to the advantages and disadvantages ofstructure of solar collectors, developed a new type of heat transfer fluid plate solar collectors, and test its performance. Measured results, it can achieve the thermal efficiency of 0.45, higher than the conventional flat solar collector Shows that the heat transfer fluid superior performance, have a good prospect.%针对市场上的平板太阳能集热器结构的优缺点,研制了新型的导热油平板太阳能集热器,并测试其性能.实测结果为,热效率能达到0.45,比普通平板集热器高.表明该导热油平板太阳能集热器性能优越,有很好的应用前景.

  14. Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants

    Directory of Open Access Journals (Sweden)

    Dashrath B. Sadhu

    2015-05-01

    Full Text Available Aim: The aim was to study the seroprevalence and efficacy of the different serological tests used for detection of antibody against Brucella species in small ruminants of Banaskantha district of North-Gujarat. Materials and Methods: Total 1000 serum samples comprising of 485 from sheep and 515 from goat tested for detection of antibodies against the Brucella species by three different serological tests viz., Rose bengal plate test (RBPT, Standard tube agglutination test (STAT, and Indirect Enzyme-linked immunosorbent assay (I-ELISA. Results: The seroprevalence of brucellosis in small ruminants was 11.30%, 11.10%, and 8.80% by RBPT, STAT, and I-ELISA, respectively. The seroprevalence of brucellosis was found to be higher in sheep than goats. The sensitivity of RBPT was found slight more than STAT, but the specificity of both tests was same. In this study, the overall agreement of RBPT and STAT with I-ELISA was found 92.50% and 92.30% in small ruminants, respectively. Conclusion: I-ELISA was a better serological test as compared to RBPT and STAT in the sense of sensitivity, specificity, and rapidity and it could be advocated for screening of brucellosis in sheep and goats.

  15. 一种鉴别与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌的PCR方法%A PCR method of identification for Citrobacter freundii agglutinating with O157 serum

    Institute of Scientific and Technical Information of China (English)

    卢珊; 白莉; 熊衍文

    2009-01-01

    Objective To develop a PCR method to distinguish Citrobacter freundii which agglutinate with O157 serum from Escherichia coil O157. Methods All the strains isolated were subjects for biochemical identification, test for agglutination with O157 serum and test for wzx gene by PCR. Results The wzx gene was detected in 25 strains of Citrobacterfreundii which showed agglutination reaction with O157 serum, while no wzx gene was detected in 8 strains of Citrobacterfreundii which showed no agglutination reaction with O157 serum. No wzx PCR product was detected in 20 strains of O157: H7. Conclusion The wzx-basod PCR is a sensitive and rapid method to distinguish Citrobacterfreundii strains which agglutinate with OI57 serum from Escherichia coli O157 strains.%目的 利用基于O抗原翻转酶wzx基因的PCR方法,鉴别与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌和O157大肠埃希菌.方法 用微生物鉴定仪对细菌进行生化鉴定,与P157血清凝集,同时扩增wzx基因.结果 25株与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌及1株不与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌wzx基因扩增为阳性,8株与O157血清不凝集的弗氏枸橼酸杆菌wzx基因扩增为阴性,20株O157:H7大肠埃希菌wzx基因扩增为阴性.结论 对于可与O157血清发生强凝集的弗氏枸橼酸杆菌,基于O抗原翻转酶wzx基因的PCR方法是一种有效的快速鉴别方法.

  16. Performance of four different agar plate methods for rectal swabs, synergy disk tests and metallo-β-lactamase Etest for clinical isolates in detecting carbapenemase-producing Klebsiella pneumoniae.

    Science.gov (United States)

    Papadimitriou-Olivgeris, Matthaios; Vamvakopoulou, Sophia; Spyropoulou, Αikaterini; Bartzavali, Christina; Marangos, Markos; Anastassiou, Evangelos D; Spiliopoulou, Iris; Christofidou, Myrto

    2016-09-01

    The aims of the study were to compare four different agar plate methods in the identification of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) from rectal samples and to assess the role of phenotypic methodologies in the identification of carbapenemase type from clinical K. pneumoniae isolates. Two chromogenic agars (Brilliance CRE and CHROMagar KPC) were compared to MacConkey agar plates with ertapenem (ERT) or imipenem (IMP) disks for the identification of CP-Kp from 912 rectal swabs. CP-Kp was detected in 329 samples by either agar methodology (299 K. pneumoniae carbapenemase positive, 27 Verona integron-encoded metallo-β-lactamase positive and 3 K. pneumoniae carbapenemase and Verona integron-encodedmetallo-β-lactamase positive). Sensitivity of Brilliance CRE, CHROMagar KPC and MacConkey agar plus IMP or ERT disk (inhibition zone carbapenemase type as compared to PCR results (blaVIM, blaKPC and blaNDM) from 2515 isolates with reduced susceptibility to any of the Etest-examined carbapenems (ERT, IMP or meropenem). Metallo-β-lactamase MP/MPI Etest was applied in 616 isolates. Sensitivity was 98.4, 90.9 and 82.2 % for phenylboronic acid synergy test, EDTA synergy test and metallo-β-lactamase Etest, respectively, while their specificity was high (>97.5 %). Phenotypic methodologies can provide reliable results for the identification of carbapenemase production among K. pneumoniae isolates. Chromogenic agars can be applied in high-risk patients as part of surveillance and infection control programs.

  17. Direct Agglutination Test and Enzyme Linked Immunosorbent Assay with Urine Samples for the Diagnosis of Visceral Leishma-niasis

    Directory of Open Access Journals (Sweden)

    Sarkari B

    2007-07-01

    Full Text Available Background: Visceral leishmaniasis (VL or Kala azar is an infectious disease caused by various species of Leishmania parasites. The aim of this study was to detect and compare the presence of anti-Leishmania antibodies in the urine of vis-ceral leishmaniasis patients using ELISA and DAT methods."nMethods: A total of 30 urine samples were collected from VL patients referred to Shiraz (southeast of Iran hospitals. Moreover 31 urine samples were collected from healthy individuals and patients with other diseases such as malaria, brucellosis, hydatidosis and cutaneous leishmaniasis. Collected samples were examined to detect anti-Leishmania antibod-ies in urine, using ELISA and DAT."nResults: Anti-Leishmania antibody was detected in urine of 18 out of 30 (60% VL patients by DAT while ELISA detected anti-Leishmania antibodies in urine of 28 out of 30 (93.3% of VL cases. Sensitivity and specificity of urine-based DAT was 60% and 83.9%, respectively while sensitivity and specificity of urine-based ELISA were 93.3% and 93.5%, corre-spondingly. "nConclusion: Urine-based DAT and ELISA have a reasonable specificity and sensitivity in diagnosis of VL. Accordingly, urine-based ELISA might be a suitable alternative for serum based assays for diagnosis of VL.

  18. License Plate Recognition for Parking Control System by Mathematical Morphology

    Institute of Scientific and Technical Information of China (English)

    Javier Ortiz; Alberto Gómez

    2014-01-01

    Nowadays, license plate recognition for parking systems is a critical task to provide automatic control of customers and payment. This paper introduces a new method for automatic recognition of license plates of vehicles by mathematical morphology. The proposed method can provide the license plate number of the plates in different light conditions, colors, sizes, and inclination (angles). The algorithm can recognize the license plates of European Union vehicles quickly and correctly. The pattern learning of mathematical skeletons has high efficiency in the process. The performance of the algorithm is demonstrated well by the test in a parking control system.

  19. Create Your Plate

    Medline Plus

    Full Text Available ... blood glucose levels and lose weight. With this method, you fill your plate with more non-starchy ... but changes the portion sizes so you are getting larger portions of non-starchy vegetables and a ...

  20. Create Your Plate

    Medline Plus

    Full Text Available ... blood glucose levels and lose weight. With this method, you fill your plate with more non-starchy ... 4/Box) Taking the guesswork out of portion control has never been easier. It can be a ...