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Sample records for plantlets cv grande

  1. STUDIES ON HARDENING AND ACCLIMITIZATION OF MICROPROPAGATED PLANTLETS OF BANANA CV. GRAND NAINE

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    S AHMED

    2016-09-01

    Full Text Available The experimental plant material of cv. Grand Naine was raised through tissue culture on MS medium using suckers as explants. The MS medium supplemented with BAP 4.00 mg/L+ IAA 2.00 mg/L was used for shoot multiplication. The rooting was obtained on MS (half strength medium fortified with IBA 1.00 mg/L and activated charcoal 200 mg/L. The in vitro rooted plantlets were hardened and acclimatized by using different treatments. Plants transplanted at the age of 4 weeks after root initiation gave maximum survival (100.00 % during transplanting. These plants were hardened in glass beaker and polythene bags singly or in cluster. The maximum survival during hardening (100.00 % was observed by covering the plantlets with glass beaker individually and kept in culture room. Out of various potting mixture tried, the potting mixture containing soil: sand and FYM (2:1:1 v/v/v gave maximum height and survival of plantlets. The results showed that out of different potting mixtures used for hardening soil: sand and FYM (2:1:1 v/v/v showed cent percent survival.

  2. Shading and periods of acclimatization of micropropagated banana plantlets cv. Grande Naine Sombreamento e períodos de aclimatização de mudas micropropagadas de bananeira cv. Grande Naine

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    Ciro Scaranari

    2009-06-01

    Full Text Available Banana plantlets obtained by micropropagation need to be submitted to a period of acclimatization since they do not use light, water, and nutrients in an efficient way. The acclimatization must be carried out under greenhouse conditions where temperature, light, and air humidity are adequate for a gradual hardening of the plantlets. In this study, the development of banana plantlets was evaluated during acclimatization under a full light condition including covered surfaces with red shade cloth (70%, 50%, and 30% shade and black shade cloth (50% shade, both under a transparent plastic film of 100 µm. Temperature, relative air humidity, irrigation, and nutrition conditions were also controlled. Physical and physiological parameters were recorded at various stages in the greenhouses after three, six, and nine weeks and also after seven weeks of transplanting to field conditions. Treatments were hierarchically graded according to their statistic classification. Combined results indicated superior outcomes of plantlets maintained under black 50% shade cloth for nine weeks, both in the summer and winter seasons. Similar results, but in a shorter time, were obtained with plantlets cultivated under red 70% shade cloth, for six weeks in the summer.Após a sua obtenção em laboratório, mudas in vitro necessitam passar por um período de aclimatização, pois na fase em que se encontram não realizam eficientemente a absorção de luz, água e nutrientes, devendo ser feita em ambiente protegido, onde as condições de temperatura, umidade e luminosidade são favoráveis a um gradual endurecimento das plântulas. No presente trabalho estudou-se o desenvolvimento das mudas de bananeira (Musa sp. durante o segundo estágio da aclimatização (a partir de 10 cm de altura sob condições de luminosidade (plena, 70, 50 e 30% de superfície de cobertura com malha vermelha e 50% com malha preta, ambas sob filme plástico transparente de 100 µm associadas a

  3. Induction of shoot buds, multiplication and plantlet formation in seedling explants of bell pepper (Capsicum annuum L. cv. Bryza in vitro

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    Andrzej Gatz

    2014-01-01

    Full Text Available In vitro shoot bud induction and multiplication as well as plantlets formation from different parts of 21-d old seedlings (shoot tip, cotyledonary node, distal part of cotyledon, acropetal section of hypocotyl of Capsicum annuum L., cv. Bryza were compared. During 4 weeks of primary explant culture on initiation media, first shoot bud primordia appeared; they reminded leaf primordia and subsequently some of them underwent enlargement, some developed into leaves and leaf-like structures (mainly on cotyledon explants. The highest number of shoot bud primordia was noted on cotyledonary node explants, but they were smaller than those on the remaining types of the explants. The best response of shoot regeneration showed cotyledon explants on which most of shoot buds were formed in each from four treated passages. From shoot buds on elongation media after 4 weeks of culture rooted rosettes of leaves were achieved, and the extension of the culture time to eight weeks without subculture caused that the rosettes developed into plantlets. Throughout four successive passages plantlets were obtained from cotyledon and shoot tip explants.

  4. Adubação foliar com macro e micronutrientes no crescimento de mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L. Merrill] em diferentes recipientes Foliar fertilization with macro and micronutrients in the growth of plantlets micropropagated of pineapple cv. Gold [Ananas comosus (L. Merrill] in different containers

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    Izaias dos Santos Bregonci

    2008-06-01

    Full Text Available Objetivou-se com este trabalho avaliar o efeito da adubação foliar com macro e micronutrientes no crescimento das mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L. Merrill], em diferentes recipientes. O experimento foi em esquema fatorial 8x3, adubação foliar em 8 níveis e recipientes em 3 níveis, através de um delineamento inteiramente casualizado com 5 repetições. As mudas foram padronizadas com altura média de 7,12 cm. As adubações foliares foram feitas com uréia, cloreto de potássio, ácido bórico, um formulado comercial com macro e micronutrientes e testemunha (pulverização com água e os recipientes: bandeja de isopor com 200 células; tubete pequeno de 115 cm³; e tubete grande com 300 cm³. O substrato utilizado foi o plantmax hortaliças®. Avaliaram-se as características área foliar, altura de planta e massa seca da parte aérea e da raiz, aos 140 dias do transplantio. Os adubos foliares proporcionaram maior crescimento em área foliar, altura e massa seca da parte aérea às mudas do abacaxizeiro, embora com resultados diferentes. Os adubos foliares não aumentaram a massa seca do sistema radicular. A bandeja de isopor apresentou as menores médias, com todos os adubos foliares para área foliar, altura e massa seca da parte aérea das mudas do abacaxizeiro. O tubete pequeno e o tubete grande apresentaram resultados semelhantes com a maioria dos adubos foliares utilizados.The objective of this work was to evaluate the effect of the foliar fertilization with macro and micronutrients on the growth of the plantlets micropropagated of pineapple cv. Gold [Ananas comosus (L. Merrill] in different containers. The experiment was mounted in factorial arrangement 8x3, with foliar fertilization in 8 levels and containers in 3 levels, through a completely randomized design with five repetitions. The plantlets were standardized with average height of 7,12 cm. The foliar fertilization was used in the urea

  5. Aib e substrato na produção de mudas de mirtilo cv. "Climax" através de microestaquia Iba and substrate in the plantlets production of blueberry cv. 'Climax' through microcuttings

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    Márcia Wulff Schuch

    2007-10-01

    Full Text Available Embora no Sul do Brasil o mirtilo esteja sendo pesquisado e produzido como uma nova alternativa na fruticultura, a maioria das mudas produzidas são obtidas pela propagação vegetativa, através do uso de estacas, e os resultados obtidos s��o insatisfatórios. Dessa forma, o objetivo deste trabalho foi testar a capacidade de enraizamento de mirtilo "rabbiteye", através da técnica de microestaquia. Microestacas provenientes de plantas aclimatadas de Vaccinium ashey Reade, cv. "Climax", obtidas através de micropropagação, foram testadas. O experimento foi conduzido em casa de vegetação usando dois tipos de microestacas (média e apical, dois substratos (areia e Plantmax® e três concentrações de AIB (0, 1000 e 2000mg L-1. Aos 60 dias após a instalação do experimento, foi avaliada a percentagem de microestacas enraizadas, a percentagem de microestacas sobreviventes, o número médio de raízes por microestaca, o comprimento médio das raízes e o comprimento da raiz mais desenvolvida. Concluiu-se que a microestaquia pode ser uma técnica eficiente na produção de mudas de mirtilo, através do uso de plantas-matrizes micropropagadas. O emprego de microestacas oriundas da porção mediana proporcionou elevados índices de enraizamento e sobrevivência, independentemente da concentração de AIB utilizada. Por outro lado, para microestacas apicais, a aplicação de AIB foi fundamental para o enraizamento.Although blueberries in the South of Brazil have been researched and produced as a new alternative in the horticulture, the majority of plants are propagated vegetatively through the use of cuttings and the results obtained are unsatisfactory. In this way, this research was aimed at testing the rooting ability of rabbiteye Blueberry by microcuttings techniques. Microcuttings from acclimated plants of Vaccinium ashey Reade cv. Climax obtained through micropropagation were tested. The experiment was carried out in a greenhouse using

  6. Comparison of total Hg results in sediment samples from Rio Grande reservoir determine by NAA and CV AAS

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    Franklin, Robson L., E-mail: robsonf@cetesbnet.sp.gov.br [Centro Tecnologico de Saneamento Basico (ELAI/CETESB), SP, Sao Paulo (Brazil). Laboratorio de Quimica Inorganica; Bevilacqua, Jose Eduardo [Centro Tecnologico de Saneamento Basico (CETESB), SP, Sao Paulo (Brazil). UNIFIEO - Centro Universitario FIEO; Favaro, Deborah I.T., E-mail: defavaro@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Laboratorio de Analise por Ativacao Neutronica (LAN/CRPq)

    2011-07-01

    The Rio Grande reservoir is located in the Metropolitan area of Sao Paulo and it is used for recreation purposes and as source water for drinking water production. During the last decades has been detected mercury contamination in the sediments of this reservoir, mainly in the eastern part, near the main affluent of the reservoir, in the Rio Grande da Serra and Ribeirao Pires counties. In the present study bottom sediment samples were collected in four different sites into four sampling campaigns during the period of September 2008 to January 2010. The samples were dried at room temperature, ground and passed through a 2 mm sieve. Total Hg determination in the sediment samples was carried out by two different analytical techniques: neutron activation analysis (NAA) and cold vapor atomic absorption spectrometry (CV AAS). The methodology validation, in terms of precision and accuracy, was performed by reference materials, and presented a recovery of 83 to 108%. The total Hg results obtained by both analytical techniques ranged from 3 to 71 mg kg-1 and were considered similar by statistical analysis, even though NAA technique furnishes the total concentration while CV AAS using the 3015 digestion procedure characterizes only the bioavailable Hg. These results confirm that both analytical techniques were suitable to detect the Hg concentration levels in the Rio Grande sediments studied. The Hg levels in the sediment of the Rio Grande reservoir confirm the anthropogenic origin for this element in this ecosystem. (author)

  7. Comparison of Grand Median and Cumulative Sum Control Charts on Shuttlecock Weight Variable in CV Marjoko Kompas dan Domas

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    Musdalifah, N.; Handajani, S. S.; Zukhronah, E.

    2017-06-01

    Competition between the homoneous companies cause the company have to keep production quality. To cover this problem, the company controls the production with statistical quality control using control chart. Shewhart control chart is used to normal distributed data. The production data is often non-normal distribution and occured small process shift. Grand median control chart is a control chart for non-normal distributed data, while cumulative sum (cusum) control chart is a sensitive control chart to detect small process shift. The purpose of this research is to compare grand median and cusum control charts on shuttlecock weight variable in CV Marjoko Kompas dan Domas by generating data as the actual distribution. The generated data is used to simulate multiplier of standard deviation on grand median and cusum control charts. Simulation is done to get average run lenght (ARL) 370. Grand median control chart detects ten points that out of control, while cusum control chart detects a point out of control. It can be concluded that grand median control chart is better than cusum control chart.

  8. Somaclonal variation in micropropagated Heliconia bihai cv. Lobster Claw I plantlets (Heliconiaceae Variação somaclonal em mudas micropropagadas de Helicônia, Heliconia Bihai cv. Lobster Claw I (Heliconiaceae

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    Paulo Hercílio Viegas Rodrigues

    2008-12-01

    Full Text Available The occurrence of somaclonal variation is described in various cultures of agronomic interest. Such variation can be of benefit in the development of new flower varieties. In this study, the occurrence of somaclonal variation in micropropagated changes of Heliconia bihai cv. Lobster Claw I was investigated. Stem apexes were introduced in MS culture media with the addition of 2.5 mg L-1 of benzylaminopure (BAP and 500 mg L-1 of sodium cefotaxime. After selecting the apex stem, it was sub-cultivated in MS media and supplemented with 4.0 mg L-1 of BAP to induce side buds. To conduct the trial, 2,000 plants were selected and compared with plants originated from rhizomes. To calculate the percentage of the variants, the plant stature, the form and color of leaves and pseudostem were evaluated. The plants with buds presenting the same type of variation were considered as variants. The occurrence of three types of somaclonal variants was observed: Variation of the Chlorophyll in the Leaf, Low Stature Variant and Pseudostem and Petiole Color Variant, the latter with ornamental potential. The somaclonal variation rate for Heliconia bihai cv Lobster Claw I, under the proposed conditions, was 61.40%.A ocorrência de variação somaclonal é descrita em diversas culturas de interesse agronômico. A floricultura pode beneficiar-se dessa variabilidade, com a obtenção de novas variedades. Nesse trabalho, estudou-se a ocorrência de variação somaclonal em mudas micropropagadas de Heliconia bihai cv. Lobster Claw I. Ápices caulinares foram introduzidos em meio de cultivo MS com adição de 2,5 mg L-1 de benzilaminopurina (BAP e 500 mg L-1 de cefotaxima sódica. Após a seleção do ápice caulinar, o explante foi subcultivado em meio MS suplementado com 4,0 mg L-1 de BAP para indução de brotações. Foram selecionadas, ao acaso, 2.000 mudas e comparadas com mudas originadas de rizomas, para compor o ensaio. No cálculo da porcentagem dos variantes

  9. Crinivirus and begomovirus detection in tomato plantlets and weeds associated to nurseries in Cartago province

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    The aim of this work was to detect plant infections caused by Tomato chlorosis virus (ToCV) and begomoviruses in tomato plantlets, as well as in weeds growing around nursery greenhouses. During one year, starting in April 2008, 168 leaf tissue samples were collected, 90 tomato plantlets and 78 weed...

  10. Automatic Dissection Of Plantlets

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    Batchelor, B. G.; Harris, I. P.; Marchant, J. A.; Tillett, R. D.

    1989-03-01

    Micropropagation is a technique used in horticulture for generating a monoclonal colony of plants. A tiny plantlet is cut into several parts, each of which is then replanted. At the moment, the cutting is performed manually. Automating this task would have significant economic benefits. A robot designed to dissect plants would need to be equipped with intelligent visual sensing. This article is concerned with the image acquisition and processing techniques which such a machine might use. A program, which can calculate where to cut a plant with an "open" structure, is presented. This is expressed in the ProVision language, which is described in another article presented at this conference. (Article 1002-65)

  11. Efect of intercellular extracts from banana inoculated leaves with Mycosphaerella fijiensis Morelet, on chloroplast electronic transport of Grande naine (AAA cv.

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    Michel Leiva-Mora

    2003-01-01

    Full Text Available Some foliar pathogens colonize intercellular spaces of damage tissues during infection process, mediated by toxins production and diffusion to kill adjacent healthy cells. Due to the absence of reliable bioassays, the physiologic effects of several phytotoxins are still ignored on cellular membranous systems of the affected cells. In the present work it was extracted the intercellular content from not inoculated and inoculated banana leaves with different Mycosphaerella fijiensis strains. Their effects on chloroplasts of Grande naine cv were evaluated by the absorbance evolution (595 nm of Hill reactive (DCPIP, mixture with 810 ì l of chloroplasts suspension and 99 ì l of the intercellular contents. The electronic exchange on chloroplasts suspension was inhibited by intercellular contents of inoculated leaves. The intercellular contents from leaves inoculated with I1 (high virulence strain had a major inhibiter effect respect to leaves inoculates with G1 strain (low virulence, showing a correspondence between the inhibiter effect of intercellular contents and the affection levels of affected tissues. The procedures used in this work will let to make studies concerned with Mycosphaerella fijiensis-Musa spp interactions and the future breeding programs. Key words: banana breeding, black Sigatoka, host pathogen interaction, physiological bioassays

  12. 江西铅山红芽芋胚性愈伤组织的玻璃化法超低温保存及植株再生%Cryopreservation of Jiangxi Yanshan red bud taro (Colocasia esculenta var.cormosus cv.Hongyayu) embryogenic callus by vitrification and its plantlet regeneration

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    洪森荣; 尹明华; 王艾平

    2014-01-01

    以江西铅山红芽芋胚性愈伤组织为材料,研究各种因素对其玻璃化法超低温保存的影响.结果表明:江西铅山红芽芋胚性愈伤组织玻璃化法超低温保存较佳的预培养条件为0.3 molL-1蔗糖预培养3 d,较佳的60% PVS2装载时间为20 min,较佳的100% PVS2脱水条件为25℃脱水30 min,较佳的化冻温度为40℃,较佳的洗涤液蔗糖浓度为1.2 molL-1,较佳的冻后培养条件为暗培养7 d 再转到光周期中培养.红芽芋胚性愈伤组织包埋玻璃化超低温保存后的平均成活率约为70%.红芽芋胚性愈伤组织冻后再生苗没有发生形态学、生理学和细胞学的变异.%Using Jiangxi Yanshan red bud taro (Colocasia esculenta var.cormosus cv.Hongyayu)embryogenic calli as materials,the effects of various factors on its cryopreservation by vitrification were studied.The results showed that better preculture condition of Jiangxi Yanshan red bud taro embryogenic calli vitrification cryopreservation was 0.3 molL-1 sucrose for 3 days.Better loading time was 60% PVS2 for 20 min.Better dehydration time of 100%PVS2 was 30 min at 25 ℃.Better thaw temperature was 40 ℃.Better sucrose concentration in washing media was 1.2 molL-1 .Better culture condition after cryopreservation was dark culture for 7 d and then transferred to the photoperiod.The average survival rate of embryogenic calli after cryopreservation by vitrification amounted to about 70%.No significant difference was observed in the morphological,physiological and cytological indexes of plantlets coming from control and cryopreserved embryogenic calli.

  13. Avaliação de substratos para produção de mudas de alface Evaluation of substrates in the production of lettuce plantlets

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    Paulo Espíndola Trani

    2007-06-01

    Full Text Available Normalmente, os substratos disponíveis no mercado são recomendados indistintamente para grande número de culturas, sem considerar suas características e necessidades nutricionais na fase da formação de mudas. O experimento foi realizado em casa de vegetação, onde foram avaliados três substratos (Plantmax, Esfagno e G-III para o desenvolvimento de mudas de alface cv. Regina. Foram realizadas as análises químicas e físicas de pH, macronutrientes, sódio e micronutrientes, sendo calculadas a capacidade de troca catiônica (CTC e a saturação por bases (V%, além das análises de condutividade elétrica (CE, capacidade máxima de retenção de água (CRA e granulometria dos substratos. A semeadura foi feita em bandejas de poliestireno expandido com 128 células cada. O delineamento utilizado foi de blocos ao acaso com três repetições. Foram avaliadas massa fresca da parte aérea e das raízes, massa seca da parte aérea e das raízes, número de folhas verdadeiras e altura média de plantas. Dentre os substratos avaliados, Plantmax demonstrou ser mais eficiente para produção de mudas de alface com melhor qualidade.Normally, the substrates available in the market are recommended indistinctly for a great number of crops, without taking into account its characteristics and nutritional requirements during the plantlet stage. The experiment was carried out in the greenhouse, where three different substrates (Plantmax, Sphagnum and G-III were evaluated in the development of lettuce cv. Regina plantlets. Chemical and physical analysis such as pH, macro and micronutrients and sodium, cation exchange capacity (CEC and base saturation (V% were calculated as well as physical analysis such as electric conductivity, maximum water holding capacity and particle size of the substrates were carried out. The sowing was done in trays of expanded polystyrene with 128 cells each. We analyzed the fresh and dry mass of the aerial parts and of the roots

  14. THE GROWTH PERFORMANCE OF CITRUS DERIVED FROM SOMATIC EMBRYOGENESIS PLANTLET AND SCION STOCK

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    Nirmala Friyanti Devy

    2014-10-01

    Full Text Available Somatic embryogenesis (SE of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L. that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.

  15. Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

    OpenAIRE

    2016-01-01

    ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholder...

  16. Crescimento e produtividade da bananeira cv. Grande Naine sob diferentes combinações de nitrato de cálcio e ureia

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    Márcio da Silva Alves

    2010-02-01

    Full Text Available A utilização de fontes nitrogenadas via fertirrigação requer que as técnicas de manejo da aplicação sejam aperfei­çoadas, de modo que os produtores possam obter o máximo benefício econômico, combinado com um menor impacto no solo ao se utilizarem esses fertilizantes. O objetivo deste trabalho foi avaliar diferentes combinações de duas fontes nitrogenadas aplicadas por fertirrigação sobre o crescimento e a produtividade da bananeira Grande Naine no primeiro, segundo e terceiro ciclos de produção. O delineamento experimental foi em blocos ao acaso, com cinco repetições em que se utilizaram seis percentagens complementares de ureia e nitrato de cálcio, aplicados via água de irrigação em sistema de gotejamento. As variáveis avaliadas foram: altura da planta, diâmetro do pseudocaule e área foliar total, além de produtividade, comprimento e diâmetro do fruto da segunda penca. Os resultados indicaram que as diferentes combinações das fontes nitrogenadas aplicadas por fertirrigação não tiveram efeito sobre a produtividade e o cresci­mento da bananeira Grande Naine.

  17. Ultrastructural and physiological responses of potato (Solanum tuberosum L. plantlets to gradient saline stress

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    Hui-Juan eGao

    2015-01-01

    Full Text Available Salinity is one of the major abiotic stresses that impacts plant growth and reduces the productivity of field crops. Compared to field plants, test tube plantlets offer a direct and fast approach to investigate the mechanism of salt tolerance. Here we examined the ultrastructural and physiological responses of potato (Solanum tuberosum L. c.v. ‘Longshu No. 3’ plantlets to gradient saline stress (0, 25, 50, 100 and 200 mM NaCl with two consequent observations (two and six weeks, respectively. The results showed that, with the increase of external NaCl concentration and the duration of treatments, (1 the number of chloroplasts and cell intercellular spaces markedly decreased, (2 cell walls were thickened and even ruptured, (3 mesophyll cells and chloroplasts were gradually damaged to a complete disorganization containing more starch, (4 leaf Na and Cl contents increased while leaf K content decreased, (5 leaf proline content and the activities of catalase (CAT and superoxide dismutase (SOD increased significantly, and (6 leaf malondialdehyde (MDA content increased significantly and stomatal area and chlorophyll content decline were also detected. Severe salt stress (200 mM NaCl inhibited plantlet growth. These results indicated that potato plantlets adapt to salt stress to some extent through accumulating osmoprotectants, such as proline, increasing the activities of antioxidant enzymes, such as CAT and SOD. The outcomes of this study provide ultrastructural and physiological insights into characterizing potential damages induced by salt stress for selecting salt-tolerant potato cultivars.

  18. Ultrastructural and physiological responses of potato (Solanum tuberosum L.) plantlets to gradient saline stress.

    Science.gov (United States)

    Gao, Hui-Juan; Yang, Hong-Yu; Bai, Jiang-Ping; Liang, Xin-Yue; Lou, Yan; Zhang, Jun-Lian; Wang, Di; Zhang, Jin-Lin; Niu, Shu-Qi; Chen, Ying-Long

    2014-01-01

    Salinity is one of the major abiotic stresses that impacts plant growth and reduces the productivity of field crops. Compared to field plants, test tube plantlets offer a direct and fast approach to investigate the mechanism of salt tolerance. Here we examined the ultrastructural and physiological responses of potato (Solanum tuberosum L. c.v. "Longshu No. 3") plantlets to gradient saline stress (0, 25, 50, 100, and 200 mM NaCl) with two consequent observations (2 and 6 weeks, respectively). The results showed that, with the increase of external NaCl concentration and the duration of treatments, (1) the number of chloroplasts and cell intercellular spaces markedly decreased, (2) cell walls were thickened and even ruptured, (3) mesophyll cells and chloroplasts were gradually damaged to a complete disorganization containing more starch, (4) leaf Na and Cl contents increased while leaf K content decreased, (5) leaf proline content and the activities of catalase (CAT) and superoxide dismutase (SOD) increased significantly, and (6) leaf malondialdehyde (MDA) content increased significantly and stomatal area and chlorophyll content decline were also detected. Severe salt stress (200 mM NaCl) inhibited plantlet growth. These results indicated that potato plantlets adapt to salt stress to some extent through accumulating osmoprotectants, such as proline, increasing the activities of antioxidant enzymes, such as CAT and SOD. The outcomes of this study provide ultrastructural and physiological insights into characterizing potential damages induced by salt stress for selecting salt-tolerant potato cultivars.

  19. Improvement of In Vitro Date Palm Plantlet Acclimatization Rate with Kinetin and Hoagland Solution.

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    Hassan, Mona M

    2017-01-01

    In vitro propagation of date palm Phoenix dactylifera L. is an ideal method to produce large numbers of healthy plants with specific characteristics and has the ability to transfer plantlets to ex vitro conditions at low cost and with a high survival rate. This chapter describes optimized acclimatization procedures for in vitro date palm plantlets. Primarily, the protocol presents the use of kinetin and Hoagland solution to enhance the growth of Barhee cv. plantlets in the greenhouse at two stages of acclimatization and the appropriate planting medium under shade and sunlight in the nursery. Foliar application of kinetin (20 mg/L) is recommended at the first stage. A combination between soil and foliar application of 50% Hoagland solution is favorable to plant growth and developmental parameters including plant height, leaf width, stem base diameter, chlorophyll A and B, carotenoids, and indoles. The optimum values of vegetative growth parameters during the adaptation stage in a shaded nursery are achieved using planting medium containing peat moss/perlite 2:1 (v/v), while in a sunlight nursery, clay/perlite/compost at equal ratio is the best. This protocol is suitable for large-scale production of micropropagated date palm plantlets.

  20. PRODUÇÃO E DESENVOLVIMENTO DA TANGERINEIRA-'MONTENEGRINA' PROPAGADA POR ENXERTIA E ESTAQUIA, NO RIO GRANDE DO SUL MANDARIN CV. 'MONTENEGRINA' PRODUCTION AND DEVELOPMENT PROPAGATED BY CUTTINGS AND GRAFTING IN RIO GRANDE DO SUL STATE, BRAZIL

    Directory of Open Access Journals (Sweden)

    GILMAR SCHÄFER

    2001-12-01

    Full Text Available A tangerineira-'Montenegrina' (Citrus deliciosa Ten., devido às boas características de sabor, conservação e colheita tardia dos frutos, é muito apreciada e cultivada no Rio Grande do Sul. Os pomares comerciais são praticamente formados por mudas enxertadas sobre Poncirus trifoliata (L. Raf. Devido à escassez de informações relativas à combinação desta cultivar com outros porta-enxertos, bem como sua propagação por estaquia, esta pesquisa objetivou estudar o comportamento inicial de plantas enxertadas sobre citrange 'Troyer' (Citrus sinensis (L. Osb. x P. trifoliata, citrumelo 'Swingle' (C. paradisi Macf. x P. trifoliata e P. trifoliata e de plantas propagadas por estaquia. O experimento foi instalado na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul - UFRGS, em junho de 1989, delineado em blocos casualisados, com cinco plantas úteis por parcela e quatro repetições. Os resultados compreenderam as primeiras safras ocorridas de 1993 a 1998. O citrumeleiro 'Swingle' foi o porta-enxerto que melhores resultados apresentou, aumentando a eficiência produtiva da tangerineira-'Montenegrina', cultivada na Depressão Central do Rio Grande do Sul, sendo que o Poncirus trifoliata diminuiu o desenvolvimento vegetativo das copas e induziu baixa eficiência produtiva. A propagação por estaquia não ofereceu vantagens em relação à enxertia sobre citrumeleiro 'Swingle' e citrangeiro 'Troyer'.The mandarin 'Montenegrina' (Citrus deliciosa Ten. yield from plants propagated either by grafting or cutting was evaluated at the 'Universidade Federal do Rio Grande do Sul' experimental agronomic station, southern Brazil, from 1993 to 1998. Three rootstocks were used in the grafted plants. The rootstocks studied were: 'Swingle' citrumelo [Citrus paradisi Macf. x Poncirus trifoliata (L. Raf.] 'Troyer' citrange (Citrus sinensis x P. trifoliata and trifoliate orange (P. trifoliata. The work was done using a randomized

  1. Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

    Directory of Open Access Journals (Sweden)

    Aurilena de Aviz Silva

    Full Text Available ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholderia silvatlantica UENF 11711, and Herbaspirillum seropedicae HRC 54, five levels of urea (0, 2, 5, 7, and 10g L-1, and five evaluation periods (30, 60, 90, 120, and 150 days following the planting of plantlets in trays, and with four replications of each treatment. An increase in the dried weight of shoots and roots of micropropagated pineapple cv. 'Vitória' plants depended on inoculation with diazotrophic bacteria, and plantlets acclimatization could be reduced by up to 57 days, depending on the concentration of urea fertilizer.

  2. Inoculation of somatic embryos of sweet potato with an arbuscular mycorrhizal fungus improves embryo survival and plantlet formation.

    Science.gov (United States)

    Bressan, W; de Carvalho, C H; Sylvia, D M

    2000-08-01

    Responses of somatic embryos of sweet potato (Ipomoea batata (L.) Poir., cv. White Star) at different developmental stages to in vitro inoculation with Glomus etunicatum (Becker and Gerdemann) (isolate INVAM FL329) were evaluated. Somatic embryos were grown in glass tubes containing sterilized vermiculite and sand. A layer of natrosol plus White's medium was used as a carrier for arbuscular mycorrhizal (AM) fungal spores. Survival of embryos inoculated with AM fungi was significantly (P < 0.05) greater than that of noninoculated embryos at the rooted-cotyledonary-torpedo and rooted-elongated-torpedo developmental stages. Mycorrhizae significantly (P < 0.05) increased plantlet formation only when inoculation occurred at the rooted-elongated-torpedo developmental stage. The growth stage at which the embryos were inserted into the glass tubes exerted a significant influence upon plantlet formation, and plantlet formation was further enhanced by inoculation with G. etunicatum. Plantlet formation was greatest at the rooted-elongated-torpedo stage. These results demonstrate that inoculation of somatic embryos with AM fungi improves embryo survival and plantlet formation, and could enhance use of somatic embryos as synthetic seeds.

  3. Measurement of Some Physiological and Biochemical Indexes of the Antisense Fragment of CYP86MF Gene Transgenic Male Sterile Plantlets in Chinese Cabbage-Pak-Choi

    Institute of Scientific and Technical Information of China (English)

    YU Xiao-lin; CAO Jia-shu; DONG De-kun

    2003-01-01

    In order to study the mechanism of the transgenic male sterility induced by the antisense fragment of gene CYP86MF, some physiological and biochemical indexes were compared between the transgenic plantlets of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) and their controls.Results showed that there was significant difference between content of the endogenous hormones in leaves and floral buds. GA3 contents in leaves of the transgenic plantlets of B. campestris L. ssp. chinensis Makino cv.Shanghai-qing and cv. Youqing were reduced by 31.72 and 46.88% respectively as compared with those of their controls, and GA3 contents in floral buds of them were reduced by 62.92 and 80.57 % respectively. In addition, ZT contents in leaves of cv. Shanghai-qing and cv. Youqing were reduced by 11.81 and 181.20%respectively as compared with those of their controls and those in floral buds were reduced by 105.94 and 128.75% respectively. But, ABA content in transgenic plantlets was significantly higher than that in nontransgenic plantlets. However, differences among respiratory rate and cyanide-resistant respiratory rate, the photosynthesic rate and the content of total flavones in floral buds were not significant. In this study, the results indicated that the gene CYP86MF might be one of the key functional genes to control fertility of Chinese cabbage.

  4. Efeito da interação entre carvão ativado e N6-benzilaminopurina na propagação in vitro de bananeira, cv. Grand Naine (AAA Interaction effect between activated charcoal and N6-benzylaminopurine in the in vitro propagation of banana, cultivar Grand Naine (AAA

    Directory of Open Access Journals (Sweden)

    Frederico Henrique da Silva Costa

    2006-08-01

    Full Text Available O carvão ativado possui a propriedade de adsorver os compostos fenólicos liberados pela oxidação dos tecidos lesionados durante o cultivo in vitro. O objetivo deste trabalho foi avaliar os efeitos da interação entre o carvão ativado e diferentes concentrações de N6-benzilaminopurina (BAP na multiplicação in vitro da bananeira, cv. Grande Naine (AAA. O meio de cultura utilizado foi o MS, solidificado com 5 g.L-1 de ágar. O cultivo foi mantido em sala de crescimento a 25±2ºC, fotoperíodo de 16 horas e intensidade luminosa de 30 mmol.m-2s-1. Foram avaliadas a presença e a ausência de carvão ativado (0 e 3 g.L-1 e quatro concentrações de BAP (0; 2; 4 e 6 mg.L-1 no meio de cultura. O delineamento foi inteiramente casualizado, com cinco repetições, em um sistema fatorial 2x4. Os explantes foram avaliados a cada 30 dias, por um período de quatro subcultivos. Após cada subcultivo, o comprimento de brotações, a taxa de multiplicação, o vigor, o nível de oxidação das brotações emitidas e o número de raízes formadas foram avaliados. Independentemente das concentrações de BAP, o carvão ativado influenciou significativamente em todas as variáveis analisadas. De maneira geral, a adição de carvão ativado afetou negativamente a taxa de multiplicação, embora tenha melhorado o vigor e o número de raízes e diminuído a oxidação dos explantes. Na ausência de carvão ativado, o BAP proporcionou as maiores taxas de multiplicação das brotações.Active charcoal has the capacity to fix phenolic compounds released by the oxidation of manipulated tissues during in vitro cultivation. The present work objective was to evaluate the interaction effects between active charcoal, combined to different BAP concentrations in the in vitro banana propagation, cv. Grand Nain (AAA. The culture medium used was the MS, solidified with 5 g.L-1 agar. Cultures were kept in a growth room at 25 ± 2ºC with a photoperiod of 16 h at 30 mmol

  5. Plant regeneration in vitro of South Pacific taro (Colocasia esculenta var. esculenta cv. Akalomamale, Aracea).

    Science.gov (United States)

    Yam, T W; Hsu, G I; Arditti, J

    1990-08-01

    Axillary bud expiants from South Pacific (Solomon Islands) taro, Colocasia esculenta var. esculenta cv. Akalomamale (Araceae) cultured on a modified Murashige-Skoog medium containing 1 mg NAA 1(-1) and TE formed callus and produced multiple plantlets. Explants died if NAA was present at levels lower than 0.1 mg 1(-1). BA was not required and may have been inhibitory. Plantlets developed faster and became larger following transfer to a hormone-free medium two weeks after the start of culture. Fully grown plants were established in a potting mix and are growing well in a greenhouse.

  6. Acclimatization and leaf anatomy of micropropagated fig plantlets

    Directory of Open Access Journals (Sweden)

    Chrystiane Fráguas Chirinéa

    2012-12-01

    Full Text Available The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.

  7. Shoot and plantlet regeneration from meristems of Dioscorea ...

    African Journals Online (AJOL)

    SERVER

    2008-04-17

    Apr 17, 2008 ... In vitro culture media capable of regenerating moderate to high shoots and/or plantlets from meristems of two yam ..... Pvt. Ltd, Naraina, New Delhi, India, pp. ... development in medicinal yam in relation to planting material and.

  8. Somatic embryogenesis and plant regeneration from immature embryo explant of papaya (Carica papaya L. cv. washington and honey dew).

    Science.gov (United States)

    Bhattacharya, J; Khuspe, S S; Renukdas, N N; Rawal, S K

    2002-05-01

    Immature zygotic embryo explants of Carica papaya were cultured on MS medium supplemented with 2,4-D (2.0 mg/l) and formed globular embryos on explants without callus formation in 4-6 weeks. Maturation and conversion of somatic embryos was also achieved on the same medium. Cotyledonary stage embryos germinated to 63.66 and 68.33% in cv. honey dew and washington respectively in MS basal medium supplemented ABA (0.5 microm/l). Robust development and proliferation of plantlet roots in vitro was obtained on MS basal medium. Hardened plantlets have 60% survival rate.

  9. Utilização de filmes de polietileno de baixa densidade(PEBD para prolongar a vida pós-colheita de morangos, cv. oso grande The use of low density polyethylene packing to prolong the postharvest life of 'oso grande' strawberries

    Directory of Open Access Journals (Sweden)

    Joel Donazzolo

    2003-02-01

    Full Text Available Desenvolveu-se um experimento com o objetivo de avaliar o efeito da atmosfera modificada (AM, utilizando filmes de polietileno de baixa densidade (PEBD, sobre a manutenção da qualidade de morangos (Fragaria ananassa L., cv. Oso Grande, acondicionados em câmara frigorífica, a 0ºC, e em câmara de climatização, a 20ºC, simulando transporte/armazenamento refrigerado e o transporte/armazenamento convencional, sob altas temperaturas respectivamente. Os morangos foram acondicionados em bandejas plásticas com capacidade para 300 g, envoltas por filme de polivinil cloreto (PVC esticável de 15 µm de espessura. Os tratamentos constituíram-se do recobrimento de seis bandejas, alojadas em armações de madeira utilizadas comercialmente para morangos, com filmes de PEBD com 22 [T2] ou 45 µm [T3] de espessura. No tratamento-controle [T1], não se utilizou filme de PEBD. Antes do fechamento dos filmes de PEBD, foi retirado o ar e, injetado CO2, até atingir pressão parcial aproximada de 15 kPa. Todos os tratamentos foram expostos às temperaturas de 0 e 20ºC, e cada temperatura foi considerada um experimento independente. O delineamento experimental utilizado foi o de blocos ao acaso, com três repetições e a unidade experimental composta por seis bandejas com 17 frutas cada uma. As avaliações foram realizadas após sete dias para o experimento a 20ºC e, após 14 dias (com mais três dias em temperatura de 20ºC, para o experimento a 0ºC. Conforme os resultados, as frutas armazenadas a 20ºC e com filmes de PEBD apresentaram menor incidência de podridões, menor índice de cor vermelha e menor acidez titulável, e também maior firmeza de polpa, em comparação com o tratamento-controle (sem filme de PEBD. Porém, a pressão parcial de CO2 atingiu cerca de 30 kPa no interior de algumas bandejas envoltas com o filme de 45 µm, ocasionando sabor alcoólico às frutas. No experimento a 0ºC, não foram verificadas diferenças significativas

  10. Pineapple (Ananas comosus cv. pérola ex vitro growth and mycorrhizal colonization affected by in vitro sucrose concentration

    Directory of Open Access Journals (Sweden)

    Alceu Kunze

    2014-09-01

    Full Text Available The aim of this study was to evaluate the effect of sucrose concentration in the culture medium on growth and on the establishment of mycorrhizas during the acclimatization of pineapple cv. Pérola. The plantlets were micropropagated in MS culture medium with 0, 10, 20 and 30 g L-1 of sucrose and then they were acclimatized during 12 weeks under greenhouse conditions, in a sandy soil - compost mixture, uninoculated or inoculated with a Rhizophagus clarus isolate. Plantlets from the culture medium with 20 g and 30 g of sucrose L-1 showed higher shoot and root biomass than those from sugar-free medium. Mycorrhizal colonization was lower in plantlets micropropagated in sucrose-free medium, but the intensity of arbuscules did not differ among treatments. In the 12-week period of acclimatization, mycorrhizal colonization had no effect on plant biomass.

  11. Organelles genome stability of wheat plantlets produced by anther ...

    African Journals Online (AJOL)

    Yomi

    2012-03-15

    Mar 15, 2012 ... detected between doubled haploid plantlets and parental plants at the level of ctDNA and mtDNA organization. ... 2009). In breeding programmes of wheat cultivars, in vitro ... employed in releasing several barley cultivars (Muñoz- ..... Cuthbert JL, Somers DJ, Brûlé-Babel AL, Brown PD, Crow GH (2008).

  12. Identification of phytochemical components of aloe plantlets by gas ...

    African Journals Online (AJOL)

    MRT Pack 20 DVDs

    2013-12-04

    Dec 4, 2013 ... ... components of aloe plantlets by gas chromatography-mass spectrometry ... number is wild harvested or cultivated for natural products prepared .... fused silica capillary column Hp- 5ms (5% phenyl : 95% dimethyl siloxane 30 M × 0.25 .... 29.11 1-Monolinoleoylglycerol trimethylsilyl ether. C27H54O4Si2.

  13. Emgu CV essentials

    CERN Document Server

    Shi, Shin

    2013-01-01

    This book provides a practical guide to Emgu CV libraries, with sample code and examples used throughout to explain the concepts clearly. Each chapter deals with a different aspect of the Computer Vision field and the implementation of that topic in Emgu CV.If you are a C# programmer working on computer vision projects, this book is for you. You should have prior experience with C#.

  14. Molecular Detection of Endophytic Bacteria on Plantlet Tissue of Sugarcane

    Directory of Open Access Journals (Sweden)

    WIWIK EKO WIDAYATI

    2007-12-01

    Full Text Available Endophytic bacteria live in plant host tissues without causing any symptoms. The aim of this study was to examine the indigenous endophytic bacteria on sugarcane plantlets produced from the young leaf cells by using tissue culture techniques. To detect the existence of endophytic bacteria in the plantlet tissue, a series of molecular method based on PCR were applied by using ribosomal intergenic spacer (RIS primer followed by 16S rDNA partial sequence and single strand conformation polymorphism (SCCP. The results showed that the molecular method could detect the existence of bacteria in the tissues. Using the same methods, the bacteria were also found in other developmental stages of sugarcane (explants, differentiated tissues and callus.

  15. New butenolides in plantlets of Virola surinamensis (Myristicaceae).

    Science.gov (United States)

    Lopes, Norberto Peporine; Santos, Pierre Alexandre Dos; Kato, Massuo Jorge; Yoshida, Massayoshi

    2004-10-01

    A phytochemical investigation in plantlets of the Brazilian medicinal tree Virola surinamensis resulted in the isolation and structural determination of four new compounds: 3-hydroxy-4-methyl-2-(11'-piperonyl-n-undecyl)-butenolide; 3-hydroxy-4-methyl-2-(7'-piperonyl-n-heptyl)-butanolide; 9'-(3,4-methylenedioxy-phenyl)-nonanoic acid and 13'-(3,4-methylene-dioxyphenyl)-tridecanoic acid. Thirteen compounds previously isolated from seeds and adult plants were also reported.

  16. A new monoterpenoid oxindole alkaloid from Hamelia patens micropropagated plantlets.

    Science.gov (United States)

    Paniagua-Vega, David; Cerda-García-Rojas, Carlos M; Ponce-Noyola, Teresa; Ramos-Valdivia, Ana C

    2012-11-01

    Chemical studies on Hamelia patens (Rubiaceae) micropropagated plantlets allowed production of a new monoterpenoid oxindole alkaloid, named (-)-hameline (7), together with eight known alkaloids, tetrahydroalstonine (1), aricine (2), pteropodine (3), isopteropodine (4), uncarine F (5), speciophylline (6), palmirine (8), and rumberine (9). The structure of the new alkaloid was assigned on the basis of 1D and 2D NMR spectroscopy, mass spectrometry, and molecular modeling.

  17. Origination of asexual plantlets in three species of Crassulaceae.

    Science.gov (United States)

    Guo, Jiansheng; Liu, Hailiang; He, Yangyang; Cui, Xianghuan; Du, Xiling; Zhu, Jian

    2015-03-01

    During asexual plant reproduction, cells from different organs can be reprogrammed to produce new individuals, a process that requires the coordination of cell cycle reactivation with the acquisition of other cellular morphological characteristics. However, the factors that influence the variety of asexual reproduction have not yet been determined. Here, we report on plantlet formation in Kalanchoe daigremontiana, Graptopetalum paraguayense, and Crassula portulacea (Crassulaceae) and analyse the effect of initiating cells on asexual reproduction in these three species. Additionally, the roles of WUSCHEL (WUS) and CUP-SHAPED COTYLEDON 1 (CUC1) in the asexual reproduction of these species were analysed through qRT-PCR. Our results indicated that pre-existing stem cell-like cells at the sites of asexual reproduction were responsible for the formation of plantlets. These cells were arrested in different phases of the cell cycle and showed different cell morphological characteristics and cell counts. The accumulation of auxin and cytokinin at the sites of asexual plantlet formation indicated their important functions, particularly for cell cycle reactivation. These differences may influence the pattern and complexity of asexual reproduction in these Crassulaceae species. Additionally, the dynamic expression levels of CUC1 and WUS may indicate that CUC1 functions in the formation of callus and shoot meristems; whereas, WUS was only associated with shoot induction.

  18. Interaction of Ulocladium atrum, a Potential Biological Control Agent, with Botrytis cinerea and Grapevine Plantlets

    Directory of Open Access Journals (Sweden)

    Sébastien Ronseaux

    2013-09-01

    Full Text Available The effectiveness of biological control agent, Ulocladium atrum (isolates U13 and U16 in protecting Vitis vinifera L. cv. Chardonnay against gray mold disease caused by Botrytis cinerea, and simulation of the foliar defense responses was investigated. A degraded mycelium structure during cultural assay on potato dextrose agar revealed that U. atrum isolates U13 and U16 were both antagonistic to B. cinerea, mainly when isolates were inoculated two days before Botrytis. Under in vitro conditions, foliar application of U. atrum protected grapevine leaves against gray mold disease. An increase in chitinase activity was induced by the presence of U. atrum isolates indicating that the biological control agents triggered plant defense mechanisms. Moreover, U13 has the potential to colonize the grapevine plantlets and to improve their growth. The ability of U. atrum isolates to exhibit an antagonistic effect against B. cinerea in addition to their aptitude to induce plant resistance and to promote grapevine growth may explain a part of their biological activity. Hence, this study suggests that U. atrum provides a suitable biocontrol agent against gray mold in grapevines.

  19. Efeitos da quitosana no desenvolvimento in vitro de videiras cv. merlot e no crescimento micelial do fungo Elsinoe ampelina Effects of the chitosan on the development of grapevines cv. Merlot and on the mycelial growth of the fungus Elsinoe ampelina

    Directory of Open Access Journals (Sweden)

    Aline José Maia

    2010-12-01

    Full Text Available Objetivou-se, neste trabalho, avaliar o efeito da quitosana no desenvolvimento in vitro de plântulas de videira cv. Merlot e sua atividade antifúngica sobre Elsinoe ampelina. No primeiro experimento, explantes da cultivar Merlot foram transferidos para meio de cultura DSD1, acrescido das concentrações 0; 25; 50,100; 150 e 200 mg L-1 de quitosana. Após 90 dias de cultivo in vitro, as plântulas foram avaliadas quanto ao número de raízes e de folhas, porcentagem de enraizamento e brotação, comprimento de raízes e de parte aérea, massa fresca da planta. No segundo experimento, incorporou-se às concentrações 0, 60, 120, 180, 240 e 300 mg L-1 de quitosana ao meio BDA, onde inoculou-se o fungo. Posteriormente, avaliou-se o crescimento micelial aos 6 e 9 dias de incubação a 25º C no escuro. No primeiro experimento para as variáveis comprimento médio da parte aérea, massa fresca da planta inteira, porcentagem de enraizamento e porcentagem de estacas brotadas houve decréscimo linear em função das concentrações de quitosana. No segundo experimento, houve efeito linear negativo em função das concentrações crescentes de quitosana, sendo que a inibição do crescimento micelial foi de 81,7%, demonstrando o grande potencial do uso de quitosana no controle da antracnose da videira.The objective of this research was to evaluate the effects of the chitosan on the development in vitro plantlets of grapevines cv. Merlot and its antifungal action on Elsinoe ampelina. In the first trial, explants of the grapevine cv. Merlot were transferred to growing medium DSD1 plus concentrations of 0; 25; 50; 100; 150 and 200 mg L-1 of the product. After 90 days of in vitro cultivation the plantlets were evaluated for root and leaf number, root and shoot length and fresh mass of roots and shoots. In the second experiment, doses of 0; 60; 120; 180; 240 and 300 mg L-1 were incorporated to the BDA media, where the fungus was inoculated. The mycelial

  20. 巴西香蕉苗静置水培营养液配方的初步筛选%Screening of the Nutrient Solution Formulas for Static Hydroponics of Banana (Musa AAA Cavendish Subgroup 'Brazil') Plantlets

    Institute of Scientific and Technical Information of China (English)

    黄小娟; 杨腊英; 谢德啸; 周端咏; 魏巍; 郭利佳; 黄俊生

    2012-01-01

    采用静置水培法,使用4种不同的营养液配方,并以清水培养和椰糠基质栽培为对照,对巴西香蕉苗进行生长比较试验。结果表明:在香蕉苗增加的生物量方面,BXM配方(巴西木植物营养液)处理与椰糠栽培的差异不显著,但与日本园试配方、Hoagland植物营养液配方、1/2 MS培养基营养元素配方和清水培养的差异显著;在株高和叶面积方面,均以椰糠栽培的最大,BXM配方处理的居次,接下来依次是Hoagland植物营养液配方、日本园试配方、1/2 MS培养基配方和清水培养。综上所述,在这4种营养液配方中,BXM配方是巴西香蕉苗生长的最适营养液配方。%In order to study water culture techniques, growths of the banana (Musa AAA cavendish subgroup cv. Brazil) plantlets under the static culture and substrate culture were 'compared. Four nutrient solutions including Hoagland solution, Murashige and Skoog (1/2MS) solution, BXM solution and RSYY solution were used to culture banana plantlets, which grew in tap water or in substrate culture (pearlite as substrate). The data showed that banana plantlets grew well in the four tested nutrient solutions, and there was a significant increase in biomass of the banana plantlets grew in static nutrient solution over that in tap water. Among the four nutrient solutions, the biomass of the banana plantlets that grew in BXM solution was next to that in substrate (pearlite) culture, and was significantly more than that in Hoagland solution, 1/2MS solution and RSYY solution. The indexes such as plant height and leaf area of banana plantlets that grew in BXM solution were largest among the four nutrient solutions, and then that grew in Hoagland solution, RSYY solution and MS solution reduced in turn. In conclusion, the banana plantlets grew best in BXM solution among the four nutrient solutions. So BXM solution is suggested, when growing banana plantlets in water culture.

  1. Grandes remolques

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1961-07-01

    Full Text Available El empleo creciente del material pesado auxiliar en la construcción de obras de ingeniería civil ha motivado la fabricación de grandes plataformas, capaces de transportar toda clase de maquinaria auxiliar. En general, este tipo de maquinaria requiere medios de transporte, pues su circulación por carreteras es lenta, obstructiva y cara, siempre que se trate de grandes distancias, caso presente en la mayoría de ocasiones en que se exige un traslado de esta maquinaria de una a otra obra.

  2. Differential alkaloid profile in Uncaria tomentosa micropropagated plantlets and root cultures.

    Science.gov (United States)

    Luna-Palencia, Gabriela R; Huerta-Heredia, Ariana A; Cerda-García-Rojas, Carlos M; Ramos-Valdivia, Ana C

    2013-05-01

    The alkaloids of Uncaria tomentosa micropropagated plantlets and root cultures were isolated and identified by NMR and mass spectrometry. Plantlets yielded pteropodine (1), isopteropodine (2), mitraphylline (3), isomitraphylline (4), uncarine F (5), speciophylline (6), rhynchophylline (7) and isorhynchophylline (8). In plantlets growing under continuous light, tetracyclic alkaloids 7 and 8 decreased from 20 ± 1.8 at 2 months to 2.2 ± 0.33 mg/g dry wt at 6 months, while the pentacyclic alkaloids 1-4 increased from 7.7 ± 1.4 to 15 ± 0.05 mg/g dry wt, supporting their biogenetic conversion. Micropropagated plantlets produced four times more alkaloids (27.6 ± 3.1 mg/g dry wt) than greenhouse plants. Plantlet roots yielded 3, 4, 8 and the glucoindole alkaloids 3α-dihydrocadambine (9) and dolichantoside (10), the last one not previously found in Uncaria.

  3. [Morphology and AFLP analysis of tetraploid plantlets of Atractylodes macrocephala].

    Science.gov (United States)

    Wang, Hong-juan; Li, Ya-ting; Xiang, Zeng-xu

    2015-02-01

    In order to investigate the genetic basis of morphological variation of tetraploid plantlets of Atractylodes macrocephala, diploid plantlets were taken as experimental material, sterile filtration colchicine was used to soak 0.5-1.0 cm long buds. The difference between morphology and stomatal of diploid and tetraploid of A. macrocephala was compared, and genome polymorphism was explored by AFLP. The results showed that the buds dipped in 0.1% colchicine solution for 36 h was optimal conditions to induce tetraploid of A. macrocephala with induction rate of 32.0%. Morphological indexes such as leaf area index, leaf length and width, the density of stomas and the number of chloroplast of tetraploid were distinctly different from diploid. Four hundred and fifty-one bands ranging with 80-500 bp were amplified with 24 pairs of primers, the rate of polymorphism was 32.59%. These amplification sites of diploid were different from tetraploid of A. macrocephala, and the differences in morphology of them were reflected in the DNA polymorphism.

  4. Viability of post acclimatized plantlets of Robusta coffee (Coffea canephora after storage

    Directory of Open Access Journals (Sweden)

    Teguh Iman Santoso

    2011-08-01

    Full Text Available This research related to the storage method of planting materials in the form of post acclimatized plantlets of Robusta coffee multiplied by somatic embryogenesis using plastic film that wraped the whole of plantlets. This information is important to support the delivery of clonal planting materials to distribution points, especially Robusta coffee plantlets viability based on condition of the container, storage period and density of plantlets. The research was conducted at Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute, located at 45 m asl. D rainfall type (Schmidt—Ferguson classification. The first experiment determind the effect of container condition and storage duration on viability of Robusta coffee plantlets. Each experimental unit contained 100 plantlets and each treatment was repeated three times with completely randomized design in factorial. The first factor was condition of storage container, i.e. airtight and non air tight. The second factor was storage period levels: 0, 5, 10, 15 and 20 days. The storage container was cardboard volume 11 dm3. The second experiment was conducted for the optimization of storage volume and storage period. Each treatment using 100 plantlets was repeated three times in completly randomized design with factorial. The first factor was storage volume of 7 dm3 and 11 dm3, the second factor was storage period levels: 0, 5, 10, 15 and 20 days. The results indicated that the maximum store period was obtained in an airtight storage treatment with 10 days, 96.3% plantlets viability, 1% fallen leaves, 3.3% water loss and not significantly different to control. For packing 100 plantlets with height 8—10 cm and leaf number 4—6 can use the volume of container store up to 7 dm3, which showed no significant difference to container volume 11 dm3 in the percentage of viability, the percentage of fallen leaves, loss of water. Key words : Robusta coffee, plantlets, storage

  5. In Vitro Selection of Peanut Somatic Embryos on Medium Containing Culture Filtrate of Sclerotium rolfsii and Plantlet Regeneration

    Directory of Open Access Journals (Sweden)

    YUSNITA

    2005-06-01

    Full Text Available Attempts to identify somaclonal variants of peanut with resistance to Sclerotium stem rot disease due to infection of S. rolfsii were conducted. The objectives of this study were to develop in vitro selection method using culture filtrates of S. rolfsii, identify culture filtrate-insensitive somatic embryo (SE of peanut after in vitro selection and regenerate peanut R0 lines originated from culture filtrate-insensitive SE. To achieve these objectives, peanut embryogenic tissues were cultured on selective medium containing various concentrations of S. rolfsii culture filtrates and sublethal concentration of the filtrates. Medium containing sublethal level of S. rolfsii culture filtrates was used to identify culture filtrate-insensitive SE of peanut. Subsequently, the selected SEs were germinated, plantlets were regenerated and preliminary tested against S. rolfsii. Results of the experiments showed that addition of S. rolfsii culture filtrates into medium for inducing peanut somatic embryos drastically reduced their growth and proliferation. S. rolfsii culture filtrates at 10% concentration has significantly reduced the number of proliferated SE per explant. However, sublethal level was achieved at 30% of culture filtrates concentration. Responses of five peanut cultivars against 30% of culture filtrates were similar, indicating they were similar in their susceptibility against S. rolfsii. A number of culture filtrate-insensitive SE were identified after culturing 1500 clumps of embryogenic tissue of peanut cv. Kelinci for three consecutive passages on medium containing 30% of culture filtrates. Germination of selected SE and regeneration of plantlet from culture filtrate-insensitive SE resulted in 50 peanut R0 lines. These lines have been grown in the plastic house and produced normal seeds for further evaluation. Results of S. rolfsii inoculation indicated the existence of chimera for insensitivity against S. rolfsii.

  6. Grandes cocinas

    Directory of Open Access Journals (Sweden)

    García de Castro, Emilio

    1957-11-01

    Full Text Available Se describen en este artículo una serie de aparatos para grandes cocinas, vistos por los autores durante un rápido viaje por Alemania. Aprovechando los datos obtenidos se analizan brevemente las necesidades de una gran cocina moderna, comentando los planos de las instalaciones en varios hoteles o instituciones de todo el mundo. La mayoría de la información.

  7. RAPD and phytochemical analysis of Thymus moroderi plantlets after cryopreservation.

    Science.gov (United States)

    Marco-Medina, Ana; Casas, José Luis

    2013-01-01

    Cryopreservation is at present the most reliable strategy to preserve plant germplasm. When aromatic plants are the object of conservation it is necessary to assess not only the genetic but also the phytochemical stability to ensure that plant material maintains its qualities after storage. In this work we present molecular and phytochemical stability data related to a previously described vitrification-based cryopreservation protocol for Thymus moroderi Pau ex Martínez. RAPD markers have been used to assess the genetic stability of T. moroderi explants and revealed 0.34 percent of variation in the cryopreserved material studied. Phytochemical data collected from GC-MS analysis of dichloromethane extracts from cryopreserved plantlets rendered a profile in which 1,8-cineole (14.5 percent), camphor (5.9 percent) and borneol (5.2 percent) were the major components. Both data confirmed the suitability of the cryopreservation protocol applied.

  8. Production of eleutherosides in in vitro regenerated embryos and plantlets of Eleutherococcus chiisanensis.

    Science.gov (United States)

    Jeong, J H; Jung, S J; Murthy, H N; Yu, K W; Paek, K Y; Moon, H K; Choi, Y E

    2005-05-01

    High frequency somatic embryogenesis of Eleutheorcoccus chiisanensis was achieved through suspension culture of embryogenic cells in hormone-free Murashige and Skoog liquid medium supplemented with 30 g sucrose l-1. Cotyledonary somatic embryos were germinated and converted into plantlets using 20 microM: gibberellic acid which were then grown in a 10 l airlift bioreactor. HPLC analysis revealed the accumulation of eleutheroside B, E and E1 in the embryos and plantlets. Thus mass production of embryos and plantlets of E. chiisanensis can be achieved in liquid cultures and the biomass produced may become an alternative source of eleutherosides.

  9. [Study on Rapid Micropropagation in Vitro Technique of Guangfeng Medicinal Yam (Dioscorea opposita) Plantlets].

    Science.gov (United States)

    Yin, Ming-Hua; Xu, Zhi-Jian; Zhang, Sheng-qin; Lv, Si-Jie; Zeng, Yan-hong; Fu, You-zhang; Hong, Sen-rong

    2015-11-01

    In order to provide methodology reference for virus-free and germplasm conservation of Guangfeng medicinal yam (Dioscorea opposita) plantlets, rapid micropropagation in vitro technique of Guangfeng medicinal yam plantlets was studied. Using the method of plant tissue culture, single factor test and flow-cytometry, the basic procedure of Guangfeng medicinal yam tissue culture was established and the DNA content of Guangfeng medicinal yam plantlets and its potted seedlings was detected. The best disinfection procedure of stems with a bud of Guangfeng medicinal yam was washed with sterile water for three times after sterilized with 70% alcohol for 20 - 30 s and then washed with sterile water for three times again after sterilized with 0.1% mercuric chloride for 10 - 12 min; The best explants of stems with a bud of Guangfeng medicinal yam was slightly woody and more mature stems witha bud; The best proliferation culture medium of stems with a bud of Guangfeng medicinal yam was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L; The best rooting culture medium of stems with a bud of Guangfeng medicinal yam was MS + NAA 0.5 mg/L; The best culture method of Guangfeng medicinal yam plantlets was liquid culture; The best transplanting matrix of Guangfeng medicinal yam plantlets was the mixture of paddy clay and fine sand (1: 2) or the mixture of perlite and vermiculite (1: 2); The DNA content between Guangfeng medicinal yam plantlets and its potted seedlings had no significant difference. A fast and efficient micropropagation in vitro technological system of stems with a bud of Guangfeng medicinal yam is established, and the flow cytometry detect results also show the genetic stability of Guangfeng medicinal yam plantlets, whose results provide the technical and theoretical basis for the large-scale production of Guangfeng medicinal yam plantlets.

  10. Development and growth of plantlets of Pinus contorta regenerated from adventitious buds

    Energy Technology Data Exchange (ETDEWEB)

    Flygh, G.; Groenroos, R.; Arnold, S. von [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Genetics; Hoegberg, K.A. [The Association for Forest Tree Breeding, Svaloev (Sweden)

    1998-11-01

    Before micropropagation techniques can be applied to a particular species, it is crucial to optimize the method and to determine how the micropropagated plants grow in the field. Adventitious shoots developed on embryos of Pinus contorta Dougl. ex Loud. after a 2 h pulse treatment with 250 {mu}M N6-benzyladenine. The time to first subculture after the pulse treatment influenced the yield of adventitious shoots. On average, 68% of the adventitious shoots had developed roots 12 weeks after treatment with 1.25 mM indole-3-butyric acid for 6 h. The auxin treatment stimulated early rooting (i.e. within 6 weeks) but had no effect on late rooting (i.e. after 6 weeks). The size of the plantlets was of importance for the survival when potted. All plantlets with a distinct stem elongated during the first growth period while some without did not. The relative height growth rate of plantlets was similar to that of seedlings. In the field the increase of height was similar for plantlets and seedlings. Plagiotropy was higher for the plantlets than for the seedlings (35 and 10% respectively). We concluded that most plantlets of P. contorta elongate normally and have a similar gross morphology to seedlings 23 refs, 8 figs, 4 tabs

  11. Pemanfataan Pupuk Daun, Air Kelapa dan Bubur Pisang sebagai Komponen Medium Pertumbuhan Plantlet Anggrek Dendrobium Kelemense

    Directory of Open Access Journals (Sweden)

    Uhwatul Hasanah

    2014-09-01

    Full Text Available Bahan alternatif alami diperlukan untuk menggantikan bahan kimia yang mahal untuk kegiatan kultur jaringan. Penelitian ini bertujuan untuk menguji pengaruh merk, konsentrasi pupuk daun, interaksinya terhadap pertumbuhan plantlet anggrek Dendrobium dan menentukan konsentrasi yang paling optimal dalam menginduksi pertumbuhan plantlet. Percobaan dilakukan dengan rancangan acak lengkap faktorial yang terdiri dari dua perlakuan yaitu merk pupuk dan konsentrasi pupuk, masing-masing dengan tiga taraf perlakuan yaitu merk pupuk (growmore, hyponex, gandasil dan konsentrasi (1 g/l, 2 g/l, 3 g/l. Pertumbuhan plantlet anggrek pada penelitian ini diukur berdasarkan parameter tinggi plantlet, jumlah daun, luas daun, jumlah akar dan panjang akar. Data dianalisis dengan anava dua arah, bila signifikan dilanjutkan dengan uji Duncan. Hasil anava menunjukkan merk pupuk berpengaruh signifikan terhadap penambahan jumlah daun, luas daun dan jumlah akar, konsentrasi pupuk berpengaruh signifikan terhadap penambahan tinggi plantlet dan panjang akar sedangkan interaksi merk pupuk dengan konsentrasi pupuk signifikan terhadap penambahan tinggi plantlet dan luas daun. Kombinasi perlakuan yang paling optimal yang menginduksi penambahan tinggi plantlet dan luas daun adalah pupuk hyponex dengan konsentrasi 2 g/l (5,40 cm dan 5,43 cm2. Untuk mendapatkan pertumbuhan plantlet paling tinggi dan luas daun paling optimal digunakan media pupuk hyponex dengan konsentrasi pupuk 2 g/l.Natural alternative materials needed to replace expensive chemicals for tissue culture activities. This study aimed to examine the influence of the brand, the concentration of foliar fertilizer, interaction on the growth of dendrobium orchid Dendrobium and determine the optimal concentration in inducing the growth of plantlets. Experiments were carried out with completely randomized factorial design consisting of two treatments, the brand of fertilizer and manure concentration, each with three levels

  12. Pemanfataan Pupuk Daun, Air Kelapa dan Bubur Pisang sebagai Komponen Medium Pertumbuhan Plantlet Anggrek Dendrobium Kelemense

    Directory of Open Access Journals (Sweden)

    Uhwatul Hasanah

    2014-09-01

    Full Text Available Bahan alternatif alami diperlukan untuk menggantikan bahan kimia yang mahal untuk kegiatan kultur jaringan. Penelitian ini bertujuan untuk menguji pengaruh merk, konsentrasi pupuk daun, interaksinya terhadap pertumbuhan plantlet anggrek Dendrobium dan menentukan konsentrasi yang paling optimal dalam menginduksi pertumbuhan plantlet. Percobaan dilakukan dengan rancangan acak lengkap faktorial yang terdiri dari dua perlakuan yaitu merk pupuk dan konsentrasi pupuk, masing-masing dengan tiga taraf perlakuan yaitu merk pupuk (growmore, hyponex, gandasil dan konsentrasi (1 g/l, 2 g/l, 3 g/l. Pertumbuhan plantlet anggrek pada penelitian ini diukur berdasarkan parameter tinggi plantlet, jumlah daun, luas daun, jumlah akar dan panjang akar. Data dianalisis dengan anava dua arah, bila signifikan dilanjutkan dengan uji Duncan. Hasil anava menunjukkan merk pupuk berpengaruh signifikan terhadap penambahan jumlah daun, luas daun dan jumlah akar, konsentrasi pupuk berpengaruh signifikan terhadap penambahan tinggi plantlet dan panjang akar sedangkan interaksi merk pupuk dengan konsentrasi pupuk signifikan terhadap penambahan tinggi plantlet dan luas daun. Kombinasi perlakuan yang paling optimal yang menginduksi penambahan tinggi plantlet dan luas daun adalah pupuk hyponex dengan konsentrasi 2 g/l (5,40 cm dan 5,43 cm2. Untuk mendapatkan pertumbuhan plantlet paling tinggi dan luas daun paling optimal digunakan media pupuk hyponex dengan konsentrasi pupuk 2 g/l.Natural alternative materials needed to replace expensive chemicals for tissue culture activities. This study aimed to examine the influence of the brand, the concentration of foliar fertilizer, interaction on the growth of dendrobium orchid Dendrobium and determine the optimal concentration in inducing the growth of plantlets. Experiments were carried out with completely randomized factorial design consisting of two treatments, the brand of fertilizer and manure concentration, each with three levels

  13. Proteome of Gluconacetobacter diazotrophicus co-cultivated with sugarcane plantlets.

    Science.gov (United States)

    dos Santos, Marise Fonseca; Muniz de Pádua, Vânia Lúcia; de Matos Nogueira, Eduardo; Hemerly, Adriana Silva; Domont, Gilberto Barbosa

    2010-03-10

    Gluconacetobacter diazotrophicus is a micro-aerobic bacterium able to fix atmospheric nitrogen in endophytic mode. A proteomic approach was used to analyze proteins differentially expressed in the presence and absence of sugarcane plantlets. Two-dimensional gel electrophoresis (2-DE) showed 42 spots with altered levels of expression. Analysis of these spots by matrix-assisted laser desorption ionization time-of-flight in tandem (MALDI-TOF-TOF) identified 38 proteins. Differentially expressed proteins were associated with carbohydrate and energy metabolism, folding, sorting and degradation processes, and transcription and translation. Among proteins expressed in co-cultivated bacteria, four belong to membrane systems; others, like a transcription elongation factor (GreA), a 60 kDa chaperonin (GroEL), and an outer membrane lipoprotein (Omp16) have also been described in other plant-bacteria associations, indicating a common protein expression pattern as a result of symbiosis. A high protein content of 60kDa chaperonin isoforms was detected as non-differentially expressed proteins of the bacteria proteome. These results allow the assessment of the physiological significance of specific proteins to G. diazotrophicus metabolism and to the pathways involved in bacteria-host endophytic interaction.

  14. Organ and plantlet regeneration of Menyanthes trifoliata through tissue culture

    Directory of Open Access Journals (Sweden)

    Urszula Adamczyk-Rogozińska

    2014-01-01

    Full Text Available The conditions for the regeneration of plants through organogenesis from callus tissues of Menyanthes trifoliata are described. The shoot multiplication rate was affected by basal culture media, the type and concentration of cytokinin and subculture number. The best response was obtained when caulogenic calli were cultured on the modified Schenk and Hildebrandt medium (SH-M containing indole-3-acetic acid (IAA 0,5 mg/l and 6-benzyladenine (BA 1 mg/l or zeatin (2 mg/l. Under these conditions ca 7 shoots (mostly 1 cm or more in length per culture in the 5th and 6th passages could be developed. In older cultures (after 11-12 passages there was a trend for more numerous but shorter shoot formation. All regenerated shoots could be rooted on the SH-M medium supplemented with 0.5 mg/l IAA within 6 weeks; 80% of in vitro rooted plantlets survived their transfer to soil.

  15. Assessment of mint (Mentha spp. species for large-scale production of plantlets by micropropagation

    Directory of Open Access Journals (Sweden)

    Hugo Teixeira Gomes

    2015-10-01

    Full Text Available Species of the genus Mentha produce essential oils which are widely used in pharmaceutical and cosmetic industries. Current study evaluates the potential for in vitro propagation and estimates mass production of plantlets of Mentha species. Nine species (M. piperita, M. suaveolens, M. canadensis, M. longiflora, M. aquatica, M. arvensis, Mentha x gracilis, M. gracilis and M. spicata were propagated with five successive 30-day subcultures in MS medium supplemented with NAA (0.05 µM and BAP (4.4 µM. Shoots were rooted in MS with IBA, IAA or NAA (0.0; 0.25; 0.5; 2.5 or 5.0 µM. The rooted plantlets were finally acclimatized in a greenhouse. Studied species increased in multiplication rates between 4.2 and 9.0-fold per month. M. piperita, M. longiflora, M. arvensis, M. x gracilis and M. gracilis showed the greatest potential for plantlet production since the estimated production varied between 6,000 and 27,000 plantlets after five 30-days subcultures. The addition of auxin to the medium did not influence root induction. However, IAA at a concentration of 5 µM provided the best results for root length and fresh weight, with averages 11.1 cm and 0.16 g, respectively. Survival of plantlets reached 100% during the greenhouse acclimatization process.

  16. Impact of LED irradiance on plant photosynthesis and action spectrum of plantlet

    Science.gov (United States)

    Naznin, Most Tahera; Lefsrud, Mark G.

    2014-09-01

    Light emitting diodes (LEDs) can be selected to target the wavelengths absorbed by plantlets, enabling the users to customize the wavelengths of light required for maximum production. The primary purpose of this experiment was to test the effect of different ratios of red to blue LEDs on tomato plantlets photosynthetic action spectrum. Four light treatments including: red LED (100%) and three ratios of red (661 nm) to blue (449 nm) light (5:1, 10:1 and 19:1) at 60 umol m-2 s-1 for this study. The tomato plantlets cultured without blue light showed a three and half-fold decrease in photosynthesis rate. The highest photosynthetic action spectrum was observed at 10:1 but was not significantly difference from the 5:1 and the lowest action spectrum was observed at 100% red LED light. The tomato plantlets grown without the blue light showed a single-fold increase in plantlet height but were not significantly different from the 10:1 red to blue LED light. This research will allow for improved selection of LED lighting for plant tissue culture.

  17. Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture

    Directory of Open Access Journals (Sweden)

    Jingli Yang

    2013-01-01

    Full Text Available To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of torpedo-shaped SEs in a bioreactor with a 2-week subculture interval plantlets had a higher root number and leaf number and larger leaf area than did HS3 (culture of heart-shaped SEs in a bioreactor with a 3-week subculture interval and HS2 (culture of heart-shaped SEs in a bioreactor with a 2-week subculture interval plantlets. Of these converted plants, TS2 plantlets had higher survival rate (83.7% and growth characteristics after transplantation in a simple shed covered with a 50% sunshade net only for 6 months. TS2 plantlets also showed significantly lower H2O2 content and significantly increased superoxide dismutase (SOD, glutathione peroxidase (GPX, and glutathione transferase (GST expression levels as compared to HS2 plants when exposure to ex vitro conditions.

  18. Physiological responses in the banana plantlets treateds with strobilurinsRespostas fisiológicas em mudas de banananeira tratadas com estrobilurinas

    Directory of Open Access Journals (Sweden)

    Sivia Helena Modenese-Gorla da Silva

    2012-04-01

    Full Text Available There are reports that strobilurin besides having a fungicide effect can promote physiologic benefits to the plants. However, this effect on banana plants was not studied yet. The objective of the present study was to evaluate the effect of strobirulins on the physiology of banana plantlets. For this purpose, cultivar Grand Naine banana plantlets were transferred to pots containing substrate and kept in a nursery with 50% shading. The experimental design was a completely randomized design with three treatments (water, azoxystrobin and pyraclostrobin and five replications. The treatments were applied at 15, 30, 45, 60 and 75 days after transplanting at a dose 100 g a. i. ha-1 with manual spray. Plant height, pseudostem diameter, shoot dry matter in strobilurin treated plants were higher than the untreated plants, however, the effect of fungicide treatment was different, being the most pronounced effect of pyraclostrobin compared to azoxystrobin. Plants treated with pyraclostrobin had higher leaf area, nitrate reductase activity and chlorophyll content of leaf total nitrogen than the plants treated with azoxystrobin and water, which did not differ. Strobilurins affect the physiology of the banana plantlets differently, the effect being more pronounced by pyraclostrobin.Há relatos de que estrobilurinas, além de atuarem como fungicida, promovem benefícios fisiológicos às plantas. Assim sendo, o presente trabalho teve como objetivo avaliar o efeito de estrobirulinas na fisiologia de mudas de bananeira. Para tal, mudas micropropagadas da cultivar Grand Naine foram repicadas para vasos contendo substrato e mantidas em viveiro a 50% de sombreamento. O delineamento experimental foi inteiramente casualizado, com três tratamentos (água, azoxistrobina e piraclostrobina e cinco repetições. As estrobirulinas foram aplicadas com pulverizador manual aos 15, 30, 45, 60 e 75 dias após a repicagem, na dose de 100 g i. a. ha-1. A altura da planta, di

  19. Genetic transformation of calli from maize and regen-eration of herbicide-resistant plantlets

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The herbicide-resistant gene als of Arabidopsis thaliana has been transferred into embryonic calli of maize by microprojectile bombardment. We have obtained chlor-sulfuron-resistant calli and regenerated plantlets through selection by herbicide chlorsulfuron. The results of PCR analysis and Southern blotting indicate that gene als has been transferred into some plantlets. The test of spraying chlorsulfuron indicated that the transgenic plantlets had favorable herbicide-resistant trait. The purpose of the re-search was to obtain chlorsulfuron-resistant transgenic ma-ize and hope that this kind of high efficient herbicide could be widely used in rotation soil of wheat and maize. In addi-tion, through spraying herbicide, we could eliminate the hybrid plants and thereby increase the purity of F1 seeds.

  20. Use of slow-release fertilizer on the production of sweet potatoes plantlets in tray

    Directory of Open Access Journals (Sweden)

    Amarílis Beraldo Rós

    2013-12-01

    Full Text Available The production of plantlets in containers generally requires the use of fertilizers. The aim of this study was to evaluate the production of sweet potato in styrofoam trays using slow-release fertilizer. The experiment was carried out, under a screen-protected nursery, in a factorial scheme 5x5, with five doses of slow-release fertilizer NPK 19-06-10 (0, 50, 100, 150 e 200 g per 25 kg de substrate and five times of plantlets permanence in tray (14, 28, 42, 56 and 70 days after planting the cuttings. The number and dry matter of roots and leaves were evaluated. The number of roots was not influenced by fertilizer addition. In general, there is not damage to plantlets growth until the highest dose used. Therefore, the fertilizer addition increases the sweet potato plants growth and the dose of 200 g per 25 kg of substrate is responsible for the best results.

  1. Effects of Lanthanum on Root Growth and Senescence of GF43(Prunus domestica) Plantlet in Vitro

    Institute of Scientific and Technical Information of China (English)

    宋卫平; 顾福根; 于佳; 沈叶; 溪群华; 刘佳佳

    2004-01-01

    The effect and the action mechanism of lanthanum on GF43 plantlet in vitro were studied. The results of experiments show that root growth rate and dry weight of GF43 by LaCl3 treatments increase. The activities of antioxidant enzymes in root system such as superoxide dismutase(SOD),catalase(CAT) and peroxidase(POD) apparently enhance. Moreover *O-2 and malond ialdehyde(MDA) contents and cell membrane permeability of GF43 are decreased by LaCl3. The relatively stable membrane structure of cell could also be maintained and the root ageing of GF43 plantlet in vitro delays.

  2. Instant OpenCV starter

    CERN Document Server

    Dalal, Jayneil

    2013-01-01

    Get to grips with a new technology, understand what it is and what it can do for you, and then get to work with the most important features and tasks.A practical, quick, and hands-on guide for Python developers and hobbyists who want to get started with computer vision with OpenCV.This book is great for developers, hobbyists, and students new to computer vision who are looking to get a good grounding in how to use the OpenCV library. It's assumed that you will have some basic experience in C/C++ programming.

  3. Application of bioregulators for banana cv. Williams macro-propagation under thermal chamber.

    Directory of Open Access Journals (Sweden)

    Galo Cedeño-García

    2016-06-01

    Full Text Available The objective of this research was to evaluate the prolific response of corms of banana cv. Williams to the application of 6-Benzylaminopurine (6-BAP and a biostimulant based on algae extract, under thermal chamber conditions. The trial was carried out from November 2013 to April 2014 at “La Teodomira” experimental farm of the Technical University of Manabí in Ecuador. The treatments were 6-benzylaminopurine (6-BAP at concentrations of 0, 20, 40, and 80 mg/l, and a biostimulant based on algae extract at doses of 0, 20, 40, and 80 ml/corm. Benzylaminopurine was applied in the corm and the biostimulant was applied in drench. The design used was a randomized complete block with A x B factorial treatment where 6-BAP and the biostimulant were the factors; the treatments were replicated four times, for a total of 64 experimental units. Significant differences (p≤0.0001 were detected for 6-BAP factor where the higher multiplication rate was obtained at the concentration of 40 mg/l with 47.28 plantlets/corm. There were no significant differences (p≥0.9167 neither for the biostimulant nor for the 6-BAP x biostimulant interaction (p≥0.3789. Callus tissue formation was evident from first generation (R1 sprouts. At the concentration of 80 mg/l of 6-BAP, the presence of abnormal plantlets, especially coming from callus tissue, was observed.

  4. The Effects of Gamma Irradiation on the Growth and Propagation of In-Vitro Chrysanthemum Shoot Explants (cv. Yellow Puma

    Directory of Open Access Journals (Sweden)

    I. Dwimahyani

    2010-08-01

    Full Text Available The study on the effect of gamma irradiation on in-vitro shoot growth of chrysanthemum cv. Yellow Puma has been carried out. The aim of the study was to observe genetic variability of shoot growth caused by gamma irradiation. Shoot explants with four leaves were irradiated by gamma with dose of 10, 15 and 20 Gy with 3 replications at each of dose. The irradiated shoot explants were then transferred into fresh MS solid medium and placed in a growth room. Observation was performed on number of leaves and branches on M1V0 generation, while plantlets height and number of branches were observed a M1V1 generation. Number of survival plantlets and multiplication rate on three subsequent subcultures were observed as well. Results showed that gamma rays with dose of 20 Gy inhibited growth of leaves as much as 50% compared to control (shoots without irradiation, and branches 73.7% in three weeks. Observation on multiplication rate at M1V1 generation showed that gamma irradiation with dose of 10 Gy promoted multiplication rate as much as 10% higher than control. It can be concluded that in vitro mutagenesis using gamma iradiation with dose of 10 to 15 Gy can be used for inducing genetic variability of chrysanthemum cv. Yellow Puma.

  5. On a Grand Scale

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The futuristic National Grand Theater of China opened its grand curtains for a test show in September. Will this costly addition to Beijing’s entertainment scene be affordable to ordinary audiences?

  6. Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’

    Directory of Open Access Journals (Sweden)

    Kamnoon Kanchanapoom

    2010-01-01

    Full Text Available A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’ was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on ¼MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.

  7. Micropropagation of loblolly pine by somatic organogenesis and RAPD analysis of regenerated plantlets

    Institute of Scientific and Technical Information of China (English)

    唐巍

    2000-01-01

    Organogenesis was induced in callus derived from mature zygotic embryos of six families (J-56, S-1003, E-22, E-311, E-440, and Mc) of loblolly pine (Pinus taeda L.) within 24 weeks of culture. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg·L-1 indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). The most suitable medium for root formation proved to be TE medium supplemented with 0.5 mg·L-1 IBA, 2 mg·L-1 BA , and 0.5 mg/l gibberellic acid (GA3). 169 regenerated plantlets were transferred to a perlite∶peatmoss∶vermiculite (1:1:1) soil mixture, and 98 plantlets survived in the field. Total DNA was extracted from the needles of the regenerated plantlets of the six families of loblolly pine. Analysis of random amplified polymorphic DNA (RAPD) using 20 arbitrary oligonucleotide 10-mers, show that amplification products were monomorphic for all the plantlets of family J-56, S-1003, E-22, E-311, E-440, and Mc of loblolly pine. These results suggested that organogenesis can be used for clonal micropropagation of some families of loblolly pine.

  8. SIGNAL MEDIATORS AT INDUCTION OF HEAT RESISTANCE OF WHEAT PLANTLETS BY SHORT-TERM HEATING.

    Science.gov (United States)

    Karpets, Yu V; Kolupaev, Yu E; Yastreb, T O

    2015-01-01

    The effects of functional interplay of calcium ions, reactive oxygen species (ROS) and nitric oxide (NO) in the cells of wheat plantlets roots (Triticum aestivum L.) at the induction of their heat resistance by a short-term influence of hyperthermia (heating at the temperature of 42 degrees C during 1 minute) have been investigated. The transitional increase of NO and H2O2 content, invoked by heating, was suppressed by the treatment of plantlets with the antagonists of calcium EGTA (chelator of exocellular calcium), lanthanum chloride (blocker of calcium channels of various types) and neomycin (inhibitor of phosphatidylinositol-dependent phospholipase C). The rise of hydrogen peroxide content, caused by hardening, was partially suppressed by the action of inhibitors of nitrate reductase (sodium wolframate) and NO-synthase (N(G)-nitro-L-arginine methyl ester--L-NAME), and the increasing of nitric oxide content was suppressed by the treatment of plants with the antioxidant ionol and with the scavenger of hydrogen peroxide (dimethylthiourea). These compounds and antagonists of calcium also partially removed the effect of the rise of plantlets' heat resistance, invoked by hardening heating. The conclusion on calcium's role in the activation of enzymatic systems, generating reactive oxygen species and nitric oxide, and on the functional interplay of these signal mediators at the induction of heat resistance of plantlets by hardening heating is made.

  9. Pertumbuhan Plantlet Anggrek Cattleya sp. dengan Perlakuan Benzyl Amino Purine pada Media Dasar Pupuk Daun Modifikasi

    Directory of Open Access Journals (Sweden)

    HESTIN YUSWANTI

    2015-09-01

    Full Text Available Growth of Plantlets of Cattleya Orchid on The Fooliar Fertilizer-Based Medium added with Benzyl Amino Purine. The aim of the current research was to investigate the appropriate concentrationof plant growth regulator BAP on the growth of Cattleya plantlet. The experiment was utilized a Randomized Completely Design with five treatments and six replications. The basal medium used was modification of foliar fertilizer of Growmore (trade mark with addition of fish emulsion, Vitamin B1 and active charcoal. BAP concentration used as treatment were 0 ppm, 0.5 ppm, 1.0 ppm, 1.5 ppm and 2.0 ppm. Variables observed were height, leaf number, root number, root length, fresh weight and dry weight of plantlets. The result showed that concentration of 1 ppm BAP resulted in the highest value for plantlet height (5.67 cm, leaf number (4.67, root length (2.07 cm, fresh weight (0.36 g and dry weight (0.043 g.

  10. EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq. PLANTLETS DERIVED FROM TISSUE CULTURE

    Directory of Open Access Journals (Sweden)

    Sumaryono Sumaryono

    2011-10-01

    Full Text Available Plantlets of oil palm (Elaeis guineensis Jacq. derived from so-matic embryos sometimes do not form well developed-roots. Root formation of unrooted-plantlets can be induced with aux-in during ex vitro acclimatization period to simplify the proce-dure and to reduce seedling production cost. Experiments were conducted using a completely randomized design to determine the effect of different types of auxin, i.e. indole-3-acetic acid (IAA, indole-3-butyric acid (IBA, and 1-naphthalene-acetic acid (NAA at different concentrations, i.e. 0, 2, 4, 8, and 16 mM on root development of oil palm plantlets. The plantlets used were derived from somatic embryos of MK 649 oil palm clone. The basal end of the shoots was dipped in auxin solution for 10 minutes before the shoot was cultured in a small plastic pot containing a mixed growing medium. The cultures were then placed inside a closed transparent plastic tunnel (240 cm x 100 cm x 95 cm for 12 weeks. The results showed that without auxin treatment only 15% of the shoots formed roots. Dipping in auxin solution increased significantly root frequen-cy to more than 50%. The best root formation was found on the shoots treated with 2 mM NAA by which rooting frequency was 80%. Auxin treatments also increased root quality as indi-cated by more number of primary and secondary roots. IAA, IBA, and NAA treatments at all concentrations tested increased significantly shoot height on average by 42% and shoot diame-ter by 30% compared to control treatment, but did not influ-ence root length. The best treatment for inducing roots of oil palm plantlets ex vitro was by dipping the basal end of the plant-lets in 2 mM NAA solution. The result showed that rooting of oil palm plantlets could be successfully conducted ex vitro that would eliminate sterile rooting stage thus simplify the protocol and reduce seedling production time and cost.

  11. Produção de mudas de alface com biofertilizantes e substratos Production of lettuce plantlets using biofertilizer and substrates

    Directory of Open Access Journals (Sweden)

    Damiana Cleuma de Medeiros

    2007-09-01

    Full Text Available Com o objetivo de avaliar o desenvolvimento de mudas de alface que foram desenvolvidas em diferentes substratos e sob efeito de adubação foliar usando biofertilizantes, foi realizado o experimento em casa de vegetação da UFERSA. Utilizou-se o delineamento de blocos ao acaso com 4 repetições. Os tratamentos foram resultados de um fatorial 4 x 5 envolvendo tipos de substrato (composto orgânico, Gold Mix 11, areia lavada e húmus + areia lavada 1:1 e fertilizante foliar (organo-mineral comercial Fertamin, testemunha e três tipos de biofertilizantes. Foram feitas adubações foliares nas mudas, aos oito e aos 15 dias após a semeadura. O fertilizante organo-mineral comercial Fertamin apresentou os melhores resultados para massa seca da parte aérea, massa seca da raiz e número de folhas. Dentre os biofertilizantes, aquele à base de esterco de curral fresco, cinzas, leite e caldo de cana apresentou os menores valores para essas mesmas características avaliadas. O composto orgânico foi o que apresentou os maiores valores para todas as características avaliadas, exceto para comprimento da raiz.The development of lettuce plantlets was evaluated, employing different substrate types and applying foliar biofertilization. The research was done in a green house in Mossoró, Rio Grande do Norte State, Brazil. The experimental design was of randomized blocks with four replications. The treatments were composed of the interaction substract type (composed organic, Golden Mix 11, washed sand and humus + washed sand 1:1 and fertilizer (the commercial organo-mineral Fertamin, the witness and tree biofertilizer types. Plantlets were foliar fertilized 8 and 15 days after sowing date. Plantlets treated with Fertamim presented the best results in the dry matter of the aerial part, dry matter of roots and number of leaves. Among the biofertilizers, the one based on cattle manure, leached ash, milk and broth of sugar cane presented the lesser values for the

  12. High Frequency Plant Regeneration of Musa paradisiaca cv. Karibale Monthan

    Directory of Open Access Journals (Sweden)

    R. Shashi Kumar

    2015-06-01

    Full Text Available High frequency plant regeneration protocol has been standardized from banana cultivar Musa paradisiaca cv. Karibale Monthan, an endemic cultivar of Malnad region of Karnataka. The fruits are used as glomerular protective to solve kidney problems. To minimize the microbial contamination and to promote healthy growth, explants were treated with 70 % absolute alcohol for 6 min, 0.1 % Mercuric chloride for 10 min and 0.2 % for 10 min, 1 % Sodium hypochlorite for 15 min, 0.1 % Cefotaxime for 5 min and 0.05 % Gentamicin for 5 min. The high frequency shoot initiation (93.33 % was recorded at 5 mg/l BAP. The synergetic effect of BAP (4 to 6 mg/l, TDZ (0.1 to 1.2 mg/l and coconut water (0.1 to 0.9 ml/l induced multiple shoot buds and it was optimized at the concentration of 5 mg/l BAP, 0.5 mg/l TDZ and 0.5 ml/l coconut water with 15.90 ± 1.66 frequency of shoots per propagule. Supplementation of 1.0 mg/l IBA induced 5.33 ± 1.21 numbers of roots with a mean root length of 7.50 ± 1.87 roots. The 99% of plantlets with distinct roots and shoots were successfully acclimatized in the green house and transferred to the field to evaluate the agro-morphological variations. The weight of the bunch (kg, number of hands in a bunch, number of fingers in a hand, length of the finger (cm, girth of the finger (cm and girth of the pseudostem (cm exhibited by in vitro plants were higher than the in vivo plants.

  13. Proteomic Analysis of Responsive Proteins Induced in Japanese Birch Plantlet Treated with Salicylic Acid

    Directory of Open Access Journals (Sweden)

    Hiromu Suzuki

    2014-07-01

    Full Text Available The present study was performed to unravel the mechanisms of systemic acquired resistance (SAR establishment and resistance signaling pathways against the canker-rot fungus (Inonotus obliquus strain IO-U1 infection in Japanese birch plantlet No.8. Modulation of protein-profile induced by salicylic acid (SA-administration was analyzed, and SA-responsive proteins were identified. In total, 5 specifically expressed, 3 significantly increased, and 3 significantly decreased protein spots were identified using liquid chromatography/tandem mass spectrometry (LC/MS/MS and the sequence tag method. These proteins were malate dehydrogenase, succinate dehydrogenase, phosphoglycerate kinase, diaminopimalate decarboxylase, arginase, chorismate mutase, cyclophilin, aminopeptidase, and unknown function proteins. These proteins are considered to be involved in SAR-establishment mechanisms in the Japanese birch plantlet No 8.

  14. Cosmeceuticals based on Rhealba(®) Oat plantlet extract for the treatment of acne vulgaris.

    Science.gov (United States)

    Fabbrocini, G; Saint Aroman, M

    2014-12-01

    Recent evidence suggests that acne vulgaris begins as an inflammation in and around the sebaceous gland and alterations in the lipid content of sebum, which drive hyperproliferation and increased desquamation of keratinocytes within sebaceous follicles. This prevents sebum drainage, causing the formation of microcomedones, which spontaneously regress or become acne lesions when the pilosebaceous unit is further blocked by the accumulation of corneocytes. These conditions are favourable for the proliferation of Propionibacterium acnes, which further aggravates acne by enhancing abnormal desquamation, sebum production and inflammation. Also, skin fragility due to inflammation or irritation by anti-comedogenic agents can worsen the situation. Rhealba(®) Oat plantlet extract (Pierre Fabre Dermo Cosmetique) soothes and restores fragile skin in acne by reducing inflammation and inhibits bacterial adhesion of Propionibacterium acnes. Cosmeceuticals combining Rhealba(®) Oat plantlet extract and hydro-compensating actives, which are available with or without anti-comedogenic hydroxy acids, provide a balanced, multifaceted approach for acne patients.

  15. Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia 'Idaho'

    Institute of Scientific and Technical Information of China (English)

    Xie Jun-wen; Li Min; Chen Shou-yi; Sun De-you; Wang Hua-fang

    2006-01-01

    Robinia pseudoacacia 'Idaho' (Robinia × ambigua 'Idahoensis', R. pseudoacacia × R. viscosa) modified by a mtl-D gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by previous investigators. The other two stages, in vitro shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory.For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl-D gene, were assembled completely. The tissue culture plants grow well in the field.

  16. Proteomic Analysis of Responsive Proteins Induced in Japanese Birch Plantlet Treated with Salicylic Acid

    Science.gov (United States)

    Suzuki, Hiromu; Takashima, Yuya; Ishiguri, Futoshi; Yoshizawa, Nobuo; Yokota, Shinso

    2014-01-01

    The present study was performed to unravel the mechanisms of systemic acquired resistance (SAR) establishment and resistance signaling pathways against the canker-rot fungus (Inonotus obliquus strain IO-U1) infection in Japanese birch plantlet No.8. Modulation of protein-profile induced by salicylic acid (SA)-administration was analyzed, and SA-responsive proteins were identified. In total, 5 specifically expressed, 3 significantly increased, and 3 significantly decreased protein spots were identified using liquid chromatography/tandem mass spectrometry (LC/MS/MS) and the sequence tag method. These proteins were malate dehydrogenase, succinate dehydrogenase, phosphoglycerate kinase, diaminopimalate decarboxylase, arginase, chorismate mutase, cyclophilin, aminopeptidase, and unknown function proteins. These proteins are considered to be involved in SAR-establishment mechanisms in the Japanese birch plantlet No 8.

  17. Crescimento de mudas micropropagadas de bananeira aclimatizadas nas condições da Amazônia Sul Ocidental sob a influência de diferentes substratos e recipientes Growth of micropropagated banana plantlets acclimatized on conditions of South West Amazon under the influence of different substrates and recipients

    Directory of Open Access Journals (Sweden)

    Janiffe Peres de Oliveira

    2008-06-01

    . The aim of this work was to evaluate the growth of micropropagated banana plantlets during the acclimatization under the influence of different substrates and recipients on conditions of South West Amazon. Shoots of banana, cv. Grand Naine, were rooted in MS medium, being the rooting plants transferred to a nursery, in two types of plastic dibble tubes (115 cm³ and 180 cm³ and six different substrates formulated from different portions of soil, carbonized rice hulls and bovine manure. Evaluations of plant survival, height of the aerial part and pseudostem diameter were carried out each fifteen days, and at the end of 75 days, fresh and dry mass for the roots and aerial parts of the plants were also determined. It was verified that the plant survival was not influenced by the use of the 115 cm³ or 180 cm³ plastic dibble tubes. However, acclimatization accomplished in the 180 cm³ plastic dibble tubes provided larger growth in height and pseudostem diameter of the plants and, consequently, larger accumulation of fresh and dry mass of roots and aerial parts, when compared to the 115 cm³ plastic dibble tubes. Among the substrates, it was observed that bovine manure was fundamental as substrate component to obtain the best results.

  18. Identification of Discriminant Factors after Exposure of Maize and Common Bean Plantlets to Abiotic Stresses

    Directory of Open Access Journals (Sweden)

    Lázaro HERNÁNDEZ

    2015-12-01

    Full Text Available Adverse environmental conditions limit crop yield and better understanding of plant response to stress will assist the development of more tolerant cultivars. Maize and common bean plantlets were evaluated under salinity, high temperature, drought and waterlogged conditions to identify biochemical markers which could be useful for rapid identification of putative stress tolerant plants. The levels of phenolics (free, cell wall-linked, total, aldehydes including malondialdehyde and chlorophylls (a, b, total were measured on stressed plantlets.  Only two indicators were statistically non-significant:  chlorophyll b in maize plantlets stressed with sodium chloride and malondialdehyde content in drought stressed maize. The most remarkable effects of abiotic stresses can be summarized as follows: (i salinity increased levels of free phenolics in maize plantlets and chlorophylls (a, b, total in common bean; (ii high temperature (40 °C elevated levels of chlorophylls (a, b, total in maize but decreased chlorophylls (a, b, total and free phenolics in common bean; (iii drought increased phenolics and decreased chlorophylls (a, b, total in maize and increased chlorophyll pigments (a, b, total in common bean; (iv waterlogging increased free phenolics and decreased chlorophylls (a, b, total in maize and increased chlorophyll (a, total in common bean. Free phenolics and chlorophylls, especially a, were the most responsive indicators to stress and can, therefore, be considered putative biochemical markers for abiotic stress tolerance in maize and common bean. The use of Fisher’s linear discriminant analysis to differentiate non-stressed and stressed plants in breeding programs is also a novel aspect of this report. Fisher’s linear discriminant functions classified correctly 100% of non-stressed or stressed originally grouped plants.

  19. EFFECTS INDUCED BY DIETHYL SULPHATE ON SOME CYTOGENETICAL PARAMETERS AND LENGTH GROWTH OF HEMP PLANTLETS

    Directory of Open Access Journals (Sweden)

    Elena Truta

    2007-08-01

    Full Text Available The hemp seeds were treated with diethyl sulphate, in four concentrations (0.1%, 0.25%, 0.5%, and 1% and in two variants of alkylant exposure (3 and 6 hours. The length growth of plantlets, mitotic index and frequency of chromosomal aberrations were the analyzed parameters. Significant modifications were obtained at the level of parameters in variants treated with DES, comparatively with control.

  20. [Effects of lingfasu on embryoid and plantlet formation of Panax notoginseng in vitro].

    Science.gov (United States)

    Xu, Hongyuan; Meng, Aidong; Li, Chunxia; Deng, Xiqing; Zhou, Qiwei; Yang, Meichun

    2004-10-01

    On the medium MS added the right amount of 2,4-D and LFS (Angustmycin) and cultured under dark condition, the callus from stem segments of Panax notoginseng could induce a lot of embryoids. In 2-3 months, the ratio of embryoid formation reached about 90%. Then transplanted on MS + 2,4-D 1.5 mg/L + LFS 2 mg/L and cultured under light 20001x, near 30% embryoids could grow and develop as robust plantlets.

  1. Comparative physiological and proteomic analyses of poplar (Populus yunnanensis plantlets exposed to high temperature and drought.

    Directory of Open Access Journals (Sweden)

    Xiong Li

    Full Text Available Plantlets of Populus yunnanensis Dode were examined in a greenhouse for 48 h to analyze their physiological and proteomic responses to sustained heat, drought, and combined heat and drought. Compared with the application of a single stress, simultaneous treatment with both stresses damaged the plantlets more heavily. The plantlets experienced two apparent response stages under sustained heat and drought. During the first stage, malondialdehyde and reactive oxygen species (ROS contents were induced by heat, but many protective substances, including antioxidant enzymes, proline, abscisic acid (ABA, dehydrin, and small heat shock proteins (sHSPs, were also stimulated. The plants thus actively defended themselves against stress and exhibited few pathological morphological features, most likely because a new cellular homeostasis was established through the collaborative operation of physiological and proteomic responses. During the second stage, ROS homeostasis was overwhelmed by substantial ROS production and a sharp decline in antioxidant enzyme activities, while the synthesis of some protective elements, such as proline and ABA, was suppressed. As a result, photosynthetic levels in P. yunnanensis decreased sharply and buds began to die, despite continued accumulation of sHSPs and dehydrin. This study supplies important information about the effects of extreme abiotic environments on woody plants.

  2. Signal mediators at induction of heat resistance of wheat plantlets by short-term heating

    Directory of Open Access Journals (Sweden)

    Yu. V. Karpets

    2015-12-01

    Full Text Available The effects of functional interplay of calcium ions, reactive oxygen species (ROS and nitric oxide (NO in the cells of wheat plantlets roots (Triticum aestivum L. at the induction of their heat resistance by a short-term influence of hyperthermia (heating at the temperature of 42 °С during 1 minute have been investigated. The transitional increase of NO and H2O2 content, invoked by heating, was suppressed by the treatment of plantlets with the antagonists of calcium EGTA (chelator of exocellular calcium, lanthanum chloride (blocker of calcium channels of various types and neomycin (inhibitor of phosphatidylinositol-dependent phospholipase C. The rise of hydrogen peroxide content, caused by hardening, was partially suppressed by the action of inhibitors of nitrate reductase (sodium wolframate and NO-synthase (NG-nitro-L-arginine methyl ester – L-NAME, and the increasing of nitric oxide content was suppressed by the treatment of plants with the antioxidant ionol and with the scavenger of hydrogen peroxide (dimethylthiourea. These compounds and antagonists of calcium also partially removed the effect of the rise of plantlets’ heat resistance, invoked by hardening heating. The conclusion on calcium’s role in the activation of enzymatic systems, generating reactive oxygen species and nitric oxide, and on the functional interplay of these signal mediators at the induction of heat resistance of plantlets by hardening heating is made.

  3. Plantlet Regeneration of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) in Vitro Tissue Cultures.

    Science.gov (United States)

    Wang, Cheng-Long; Dong, Xue-Ni; Ding, Meng-Qi; Tang, Yi-Xiong; Zhu, Xue-Mei; Wu, Yan-Min; Zhou, Mei-Liang; Shao, Ji-Rong

    2016-01-01

    Tartary buckwheat is an ancient annual dicotyledonous herb, which is widely distributed around the world, specifically in the high altitude area of southwestern China and in the hill region of Himalayan. The plantlet regeneration of tartary buckwheat via somatic embryogenesis or multiple shoot induction was investigated in two different tartary buckwheats, Yuanzi and Xichang. The regeneration ability of Yuanzi was better than Xichang tartary buckwheat, and the hypocotyls were better than cotyledons as tartary buckwheat plantlet regeneration explants via somatic embryogenesis. The most suitable medium for callus induction was Murashige and Skoog basal medium added 2 mg/L 2, 4- dichlorophenoxyacetic acid and 1 mg/L Kinetin, which could reach up to 98.96% callus induction percentage. The plantlet regeneration percentage from callus of tartary buckwheat could reach up to 55.77%, which induced on 2.0 mg/L Benzyladenine and 1.0 mg/L KT in MS basal medium. In addition, maximum of multiple shoot induction percentage was 69.05%, which was observed in case of Yuanzi tartary buckwheat in MS basal medium with added 3.0 mg/L 6-BA and 1.0 mg/L Thidiazuron. Roots induction of regenerated plants were achieved on 1/2 MS basal medium with added 1mg/L Indole-3-Butytric acid, which has 75% survival after transferred regenerated plants to soil under field conditions.

  4. Grand Hotel prijutil hudozhnikov

    Index Scriptorium Estoniae

    2004-01-01

    Raadioajakirjanik Lea Veelmaa lindistas "Kunstikanali" 2004. a. esimese saate Grand Hotel Viljandis. Saatekülaliseks oli maalikunstnik Andres Tolts. Toltsi kaheksa akrüülmaali on eksponeeritud hotelli fuajees ja restoranis

  5. Efeito de diferentes sanificantes sobre a qualidade de morango cv. Oso Grande Effect of different sanitizing on the quality of strawberry cv. Oso Grande

    Directory of Open Access Journals (Sweden)

    Kelen Cristina dos Reis

    2008-02-01

    Full Text Available Conduziu-se o presente trabalho para estudar o efeito de diferentes sanificantes, na conservação pós-colheita de morangos, armazenados sob refrigeração (9± 1ºC e 99% UR, por um período de 12 dias. O delineamento experimental utilizado foi inteiramente casualizado, com 3 repetições, constituindo dos seguintes tratamentos: T1 Controle, imersão em água destilada, 15 min, T2 imersão em solução de dicloro s. triazinatriona sódica dihidratada (Sumaveg® a 200 ppm, por 15 min; T3 imersão em solução de peróxido de hidrogênio 10%, por 15 min; T4 imersão em solução de dicloro isocianurato de sódio (Hidrosan® a 200 ppm, por 15 min. As análises de pH, SS, AT, Cor L*a*b e microbiológicas foram realizadas a cada 3 dias. Os processos de sanificação adotados foram eficientes para manter a qualidade de morangos minimamente processados por 12 dias de armazenamento. Verificou-se, no presente trabalho, que o uso de sanificantes foi de fundamental importância para manter baixas as contagens de fungos filamentosos e leveduras dos morangos minimamente processados.The aim of this work was to study the effect of different sanitizing in the conservation postharvest of strawberry, stored in 9ºC and 99% R.H. for 12 days. The experiment was conducted in completely randomized design, with three replicates, the treatments constituted by: T1 Control, immersion in distilled water; T2 immersion in solution of dychlorine s. triazinatriona sodiumdehydrated (Sumaveg® at 200 ppm, for 15 min; T3 immersion in solution of hydrogen peroxide 10%, for 15 min; T4 immersion in solution of dychlorine isocianurate of sodium (Hidrosan® 200 ppm, for 15 min. The analyses of pH, SS (soluble solids, TA (titrable acidity, Color L*a*b and microbiological was evaluated in 0, 3, 6, 9 and 12 days. The process of sanitizing was efficient to maintain the quality of strawberry minimally processed for 12 days. The use of sanitizing was of fundamental importance to maintain low containing of microorganisms of the group mold and yeasts.

  6. In vitro sensitivity of fig plantlets to gamma rays; Sensitividade in vitro de brotacoes de figueira a radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Ester Alice [Empresa de Pesquisa Agropecuaria de Minas Gerais (EPAMIG), Uberaba, MG (Brazil). Centro Tecnologico Triangulo e Alto Paranaiba], e-mail: ester@epamig.br; Pasqual, Moacir [Universidade Federal de Lavras (UFLA), MG (Brazil). Dept. de Agricultura; Tulmann Neto, Augusto [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Lab. de Melhoramento de Plantas

    2009-07-15

    Fig breeding programs through conventional methods are rare in many countries, e.g. Brazil, since the wasp Blastophaga psenes, which is responsible for the natural pollination, is not present. For these cases a low cost alternative for the breeding program is the induction of physical mutagenesis by radiation. The sensitivity of fig explant buds of different sizes to gamma radiation were evaluated. Fig plantlets 'Roxo de Valinhos' already established in vitro were classified by size in 2.5 to 4.5 cm, 5 to 9 7 cm and 8 to 10 cm long, and irradiated with: 10, 20, 30, 40 and 50 Gy doses. After irradiation each plantlet was cut in pieces containing one-bud and transferred to WPM culture medium, according to the bud position: medium and apical. Explants were grown in a growth room for 90 days when, explant mortality, root formation, height of aerial part, number of buds and plantlet weight were evaluated. Doses of up to 50 Gy do not cause plantlet death and that doses larger than 30 Gy inhibit root formation. Therefore, the 30 Gy dose may be recommended for the irradiation of fig plantlets larger than 2.5 cm. (author)

  7. In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers.

    Science.gov (United States)

    Hrahsel, Lalremsiami; Basu, Adreeja; Sahoo, Lingaraj; Thangjam, Robert

    2014-02-01

    An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L(-1) 6-benzylaminopurine (BAP) + 0.5 mg L(-1) α-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L(-1) kinetin + 0.5 mg L(-1) NAA. While MS medium supplemented with a combination of 2 mg L(-1) BAP + 0.5 mg L(-1) NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96% survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana.

  8. Suppression of maize plantlets growth under mutual action of chromium and nickel

    OpenAIRE

    О. М. Piskova; O. M. Vinnychenko; V. М. Grishko

    2008-01-01

    Mutual toxicity of chromium and nickel sulphates for growth and development of maize plantlets was studied. Its combined action at low concentrations (10 –5 M) results to significant suppression of growth of both above-ground and root systems of maize. At the beginning of growth the greater negative effect of the salts mixture at high concentration (10 –4 M) was observed for the plantlets’ roots. Then, on the sixth day, the growth of above-ground and root system was inhibited equally. Applica...

  9. A Novel System for Proliferation,Maintenance and Plantlet Germinationfrom Somatic Embryo of Soybean

    Institute of Scientific and Technical Information of China (English)

    WANGPing; WANGGang; JIJing; WUYing

    2004-01-01

    Somatic embryogenesis was successfully induced with auxin in high concentration from immature cotyledons of 18genotypes of soybean(Glycin emax(L)Merr.)adaptable for planting in Northeast China.The frequency of somatic embryogenesis varied from 0.29%to77.62%among 18genotypes of soybean.The proliferative somatic embryos were obtained from 10genotypes of soybean at rate of 5.2%-22.1%.For the first time.plantlets were obtained even after subcultured and maintained on solid medium for over one year.This system provided a new and efficient receptor for genetic transformation of soybean.

  10. Effects of antibiotics on plantlet regeneration via organogenesis in Populus euphratica

    Institute of Scientific and Technical Information of China (English)

    Ding Xia; Chen Xiao-yang; Li Wei; Du Zhi-yan

    2006-01-01

    Four antibiotics, kanamycin, geneticin, carbenicillin and cefotaxime, were evaluated for their effect on the regeneration of adventitious buds, shoot differentiation, rooting from regenerated shoots of Populus euphratica as well as on their control of Agrobacterium-mediated transformations. Results showed that the optimum concentration ranges of kanamycin and geneticin were 15-20 and 10-15 mg·L-1 at the stage of transgenic plantlet selection. The inhibitory effects of cefotaxime and carbenicillin varied among different genotypes of Agrobacterium. The inhibition of cefotaxime on Agrobacterium C58 was stronger than that of carbenicillin.LBA4404 and their optimum concentrations were both 150 mg·L-1.

  11. Induction of callus from axillary buds of taro (Colocasia esculenta var. esculenta, Araceae) and subsequent plantlet regeneration.

    Science.gov (United States)

    Yam, T W; Young, J L; Fan, K P; Arditti, J

    1990-12-01

    Axillary buds of taro (Colocasia esculenta var. esculenta, Araceae) cultured on half strength Murashige-Skoog medium (HMS) containing taro extract (HMSTE) and 2, 4, 5-trichlorophenoxyacetic acid produce a compact, hard, slow growing callus which is not very active morphogenetically and produces only a few plantlets. When cultured on HMSTE plus 5 mg 1(-1) each of naphthaleneacetic acid and benzyl adenine (HMSNB) the buds produce a fast growing, friable and morphogenetically active callus. Meristematic regions form on the friable callus after 30 days on HMSNB. If transferred to HMSTE at this point the callus gives rise to plantlets. Addition of taro extract to the media is required for the culture of buds, induction of callus and plantlet regeneration.

  12. Comparative proteomic analysis of off-type and normal phenotype somatic plantlets derived from somatic embryos of Feijoa (Acca sellowiana (O. Berg) Burret).

    Science.gov (United States)

    Fraga, Hugo Pacheco de Freitas; Agapito-Tenfen, Sarah Zanon; Caprestano, Clarissa Alves; Nodari, Rubens Onofre; Guerra, Miguel Pedro

    2013-09-01

    Morphological disorders in a relevant portion of emerged somatic embryos have been a limiting factor in the true-to-type plantlet formation in Acca sellowiana. In this sense, the present study undertook a comparison between normal phenotype and off-type somatic plantlets protein profiles by means of the 2-D DIGE proteomics approach. Off-type and normal phenotype somatic plantlets obtained at 10 and 20 days conversion were evaluated. Results indicated 12 exclusive spots between normal and off-type plantlets at 10 days conversion, and 17 exclusive spots at 20 days conversion. Also at 20 days conversion, 4 spots were differentially expressed, up- or down-regulated. Two proteins related to carbohydrate metabolism were only expressed in off-types at 10 days conversion, suggesting a more active respiratory pathway. A vicilin-like storage protein was only found in off-types at 20 days conversion, indicating that plantlets may present an abnormality in the mobilization of storage compounds, causing reduced vigor in the development of derived plantlets. The presence of heat shock proteins were only observed during formation of normal phenotype somatic plantlets, indicating that these proteins may be involved in normal morphogenesis of plantlets formed. These new findings shed light on possible genetic or epigenetic mechanisms governing A. sellowiana morphogenesis. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  13. Anther Culture of Chinese Radish ( Raphanus sativus L. var. Longinnatus Bailey): Response of Different Genotypes to the Embryogenesis and the Traits of Regenerated Plantlets

    Institute of Scientific and Technical Information of China (English)

    ZHANG Li

    2011-01-01

    [ Objective] The aims were to ① conduct anther culture of Chinese radish varieties; ② observe the development of embryos from anther culture; ③ study the response of different genotypes to embryogenesis in anther culture; ④ observe the morphology of regenerated plantlets; ⑤ analyze the ploidy level of regenerated plantlets arising from the anther culture process. [ Method]Anthers of 15 genotypes with diverse genetic backgrounds of Chinese radish have been cultured in vitro and induced to undergo embryogenesis and plant formation. [Result] Of 15 genotypes evaluated, four produced embryos. The genotype was the main factor to influence the embryogenesis. The morphology and the ploidy of the regenerated plantlets were observed, and the anther-derived plantlets included a mix of haploids, diploids and hexaploids. Of the plants that regenerated from anther embryos 60% were diploid. [ Conclusion] The plantlets had the high ability to double spontaneously.

  14. Short Communication: Effect of mulching materials on mini tuber production of potato from in vitro plantlets

    Directory of Open Access Journals (Sweden)

    D.A.N. MAJUMDER

    2016-06-01

    Full Text Available Abstract. Majumder DAN, Nath SC, Kabir MA, Majumder S. 2016. Effect of mulching materials on mini tuber production of potato from in vitro plantlets. Nusantara Bioscience 8: 123-127. This paper describes the effectiveness of organic and plastic mulching for potato mini tuber production in Bangladesh. The field experiment was carried out during the Rabi season of 2013-2014 to 2014-2015 with virus free in vitro cultured plantlets of var. Diamant. The mulching materials of water hyacinth mulch (WHM, rice straw mulch (RSM and black polythene mulch (BPM were compared to no-mulching (control to find out suitable mulching material (s for obtaining higher tuber yield. During the whole production period morphological characters, yield characters as well as of soil temperature and soil moisture were assessed. The results showed that WHM (5.28 t/ac and RSM (4.59 t/ac had a positive effect on increased the proportion of tuber size above 28 mm and on increasing of tuber yields by 54.0% to 77.2% compared with control (2.98 t/ac. Higher soil temperatures were recorded with plastic mulch caused lower potato tuber yield (3.04 t/ac while WHM and RSM decreased soil temperatures and increased the moisture percentage. The mulching of mini tubers had negative effect on tubers quality in regards of scab, green tuber and weed biomass.

  15. Effects of mechanical vibration on root development of Actinidia chinensis plantlet

    Institute of Scientific and Technical Information of China (English)

    YANG Xiao-cheng; DING Jian-ping; WANG Bo-chu

    2007-01-01

    The root development of Actinidia chinensis plantlets was studied in exposure to environmental stress of mechanical vibration at respectively 1 Hz, 2 Hz, 3 Hz, 4 Hz and 5 Hz. The plantlets exposed to vibration stimuli at all those frequencies have a larger total number and a larger total length of roots and a smaller permeability of root plasma-membrane, compared with those cultivated in an environment without vibration stress. Vibration at respectively 1 Hz, 2 Hz, 3 Hz and 4 Hz enhances root activity and the 3 Hz vibration is the most favorable. There is an obvious negative correlation between root activity and permeability of root plasma-membrane. The effects may be explained by the likelihood that mechanical vibration at an appropriate frequency facilitates roots' absorbing water and minerals which are indispensable to inducing and synthesizing in roots some active substances favorable to growth. Nevertheless, overstress damages the integrity of root plasm-membrane, increases the permeability, and results in the disability of protecting root cells.

  16. [Infection progress of arbuscular mycorrhizae on tissue-cultured plantlets of Pinellia ternata].

    Science.gov (United States)

    Shen, Xuelian; Guo, Qiaosheng; Liu, Zuoyi; Zhu, Guosheng; Liu, Yongxiang

    2011-01-01

    To study the Arbuscular mycorrhizal (AM) formation progress and infection characteristics between tissue culture plantlets of Pinellia ternata and Glomus mosseae. The tissue culture plantlets of P. ternata were inoculated with G. mosseae, the formation of AM were sampled and observed with microscopy by staining. The hyphae of G. mosseae began to penetrate the root epidermis after 10 days of inoculation. Lots of intracellular hyphae formed in cortex cells at the 15th day. Arbuscules started to form and there were some hyphae on the root at the 20th day. At the 25th day, many arbuscules formed and most as Arum type. Some arbuscles started to disintegrate at the 30th day, and a few of vesicles occurred. Lots of spores formed after 35 days. At the 40th day, some vesicles began to decline. The hand section showed that the intercellular hyphae gradually formed in intercellular space, and the hyphae branched in cortex cells and occupied most cell lumen finally. It is expounded that P. ternata and G. mosseae could recognize each other quickly and form a symbiont system.

  17. Salt stress encourages proline accumulation by regulating proline biosynthesis and degradation in Jerusalem artichoke plantlets.

    Science.gov (United States)

    Huang, Zengrong; Zhao, Long; Chen, Dandan; Liang, Mingxiang; Liu, Zhaopu; Shao, Hongbo; Long, Xiaohua

    2013-01-01

    Proline accumulation is an important mechanism for osmotic regulation under salt stress. In this study, we evaluated proline accumulation profiles in roots, stems and leaves of Jerusalem artichoke (Helianthus tuberosus L.) plantlets under NaCl stress. We also examined HtP5CS, HtOAT and HtPDH enzyme activities and gene expression patterns of putative HtP5CS1, HtP5CS2, HtOAT, HtPDH1, and HtPDH2 genes. The objective of our study was to characterize the proline regulation mechanisms of Jerusalem artichoke, a moderately salt tolerant species, under NaCl stress. Jerusalem artichoke plantlets were observed to accumulate proline in roots, stems and leaves during salt stress. HtP5CS enzyme activities were increased under NaCl stress, while HtOAT and HtPDH activities generally repressed. Transcript levels of HtP5CS2 increased while transcript levels of HtOAT, HtPDH1 and HtPDH2 generally decreased in response to NaCl stress. Our results supports that for Jerusalem artichoke, proline synthesis under salt stress is mainly through the Glu pathway, and HtP5CS2 is predominant in this process while HtOAT plays a less important role. Both HtPDH genes may function in proline degradation.

  18. Plantlet Regeneration from the Embryo of Punica granatum cv.Nana in vitro%大花石榴胚培养再生植株

    Institute of Scientific and Technical Information of China (English)

    闵炜; 陈志萍

    2010-01-01

    以大花石榴的胚为外植体在MS+BA1+NAA0.2培养基中诱导愈伤并产生不定芽,在MS+BA0.5+NAA0.2培养基中进行芽苗的增殖培养,在1/2MS+ NAA0.5+IBA0.5培养基中诱导芽苗生根.试管苗在珍珠岩基质中罩塑料杯保湿炼苗后即可移栽入土中.

  19. Asymptotically Safe Grand Unification

    DEFF Research Database (Denmark)

    Bajc, Borut; Sannino, Francesco

    2016-01-01

    for a broad class of prime candidates of phenomenologically relevant supersymmetric grand unified theories. We also uncover candidates passing these tests, which have either exotic matter or contain one field decoupled from the superpotential. The latter class of theories contains a model with the minimal...... matter content required by phenomenology....

  20. Diez grandes arquitectos

    OpenAIRE

    Dominguez Uceta, Enrique

    2009-01-01

    Selección de 10 museos de arquitectura contemporánea realizada con Rafael Moneo y Juan Miguel Hernández León, con las mejores obras museísticas de 10 grandes arquitectos. Los edificios, de Wright, Sert, Scarpa, Piano & Rogers, Kahn, Moneo, Piano, Gehry, Siza y Herzog & de Meuron, son descritos y analizados.

  1. Study on Optimization of Jiangxi Yanshan Red Bud Taro Plantlet Chromosome Preparation Technique%江西铅山红芽芋试管苗染色制片技术优化研究

    Institute of Scientific and Technical Information of China (English)

    王艾平; 柯维忠; 林国卫; 吴燕芳; 易雪梅; 叶志康; 黄丽; 余琪; 肖淮宾

    2014-01-01

    以江西铅山红芽芋试管苗为材料,探讨不同取材部位、不同预处理剂、不同酸解离时间和不同改良卡宝品红染色时间对江西铅山红芽芋试管苗染色体制片的影响,以期为江西铅山红芽芋的起源、演化及遗传育种提供一定理论依据。结果表明,切取江西铅山红芽芋试管苗根尖,在室温下用0.1%秋水仙素+0.002mol/L 8-羟基喹啉混合溶液预处理4h ,然后在60℃条件下用1 mol/L盐酸解离15 min ,最后用改良卡宝品红染色5 min ,此时染色体制片效果最佳。%In order to better understand the information about cytology of Jiangxi Yanshan red bud taro ( Colocasia esculenta L .Schott var .cormosus cv .Hongyayu) ,provide information support for genetic breeding of red bud taro .The protocol of different sampling places ,pretreatments solutions ,dissociating time and dying time on chromosome observation from red bud taro plantlets was studied . The results showed that the effect of chromosome preparation was best when the root tips of red bud taro plantlets was cut ,pretreated in a mixture of 0 .1% colchicines and 0 .002mol/L 8-hydroxyquinoline at 25 ℃ for 4h ,dissocated in 1 mol/L HCl at 60 ℃ for 15 min and dyed with modified carbol fuchsin for 5 min .

  2. Vetiver plantlets in aerated system degrade phenol in illegally dumped industrial wastewater by phytochemical and rhizomicrobial degradation.

    Science.gov (United States)

    Phenrat, Tanapon; Teeratitayangkul, Pimpawat; Prasertsung, Isarawut; Parichatprecha, Rattapoohm; Jitsangiam, Peerapong; Chomchalow, Narong; Wichai, Siriwan

    2017-05-01

    This research evaluated the feasibility of using vetiver plantlets (Vetiveria zizanioides (L.) Nash) on a floating platform with aeration to degrade phenol (500 mg/L) in illegally dumped industrial wastewater (IDIWW). The IDIWW sample was from the most infamous illegal dumping site at Nong Nae subdistrict, Phanom Sarakham district, Chachoengsao province, Thailand. Laboratory results suggested that phenol degradation by vetiver involves two phases: Phase I, phytopolymerization and phyto-oxidation assisted by root-produced peroxide (H2O2) and peroxidase (POD), followed by phase II, a combination of phase I with enhanced rhizomicrobial degradation. The first 360-400 h of phenol degradation were dominated by phytopolymerization and phyto-oxidation yielding particulate polyphenols (PPP) or particulate organic matter (POM) as by-products, while phenol decreased to around 145 mg/L. In Phase II, synergistically, rhizomicrobial growth was ∼100-folds greater on the roots of the vetiver plantlets than in the IDIWW and participated in the microbial degradation of phenol at this lower phenol concentration, increasing the phenol degradation rate by more than three folds. This combination of phytochemical and rhizomicrobiological processes eliminated phenol in IDIWW in less than 766 h (32 days), while without the vetiver plantlets, phenol degradation by aerated microbial degradation alone may require 235 days. To our knowledge, this is the first that systematically reveals the complete phenol degradation mechanism by vetiver plantlets in real aerated wastewater.

  3. How Age of Transplants from In Vitro Derived Potato Plantlets Affects Crop Growth and Seed Tuber Yield After Field Transplanting

    NARCIS (Netherlands)

    Lommen, W.J.M.

    2015-01-01

    In vitro produced plantlets are used in potato seed systems for production of minitubers under protected conditions or for production of transplants to be transplanted to the field. Three field experiments were carried out to analyse how transplant age (Age) affected the field performance. In the

  4. Nitric Oxide and Brassinosteroids Mediated Fungal Endophyte-Induced Volatile Oil Production Through Protein Phosphorylation Pathways in Atractylodes lancea Plantlets

    Institute of Scientific and Technical Information of China (English)

    Cheng-Gang Ren; Chuan-Chao Dai

    2013-01-01

    Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide (NO) and brassinolide (BL) in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. AL12 enhanced the activities of total protein phosphorylation, Ca2þ-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein kinases in A. lancea plantlets inoculated with endophytic fungus. Pretreatments with different protein kinase inhibitors also reduced fungus-induced NO production and volatile oil accumulation, but had barely no effect on the BL level. These data suggest that protein phosphorylation is required for endophyte-induced volatile oil production in A. lancea plantlets, and that crosstalk between protein phosphorylation and the NO pathway may occur and act as a downstream signaling event of the BL pathway.

  5. In vitro callus induction and plantlet regeneration of Achyranthes aspera L., a high value medicinal plant

    Institute of Scientific and Technical Information of China (English)

    Monokesh Kumer Sen; Shamima Nasrin; Shahedur Rahman; Abu Hena Mostofa Jamal

    2014-01-01

    Objective:To study callus induction from different explants (internode, leaf, root) and in vitro plantlets propagation from medicinally important plant Achyranthes aspera L. Methods:Sterilized explants were prepared by using 0.1%HgCl2 and 0.5%Bavistin and callus was obtained when cultured onto Murashige Skoog’s (MS) medium by using different concentrations and combination of 2,4-D, NAA, BAP, IAA, IBA with 3%sucrose and 0.8%agar. Induced callus was immediately transferred to MS medium containing at different concentrations of phytohormones for shootlets and rootlets induction respectively. Results:Sterilization treatment of 0.1%HgCl2 for 2-3 min and Bavistin 0.5%for 10-12 min showed the highest percentage of asepsis and survival rate. Maximum induction of callus was obtained from a combination of 2.0 mg/L 2,4-D and 0.5 mg/L NAA from leaf. Highest shootlets number (4.83±0.17) and length (3.8±0.16) cm were observed on full strength MS medium when fortified with BAP 4.0 mg/L and KIN 0.5 mg/L. Concerted efforts of BAP 2.0 mg/L and NAA 0.5 mg/L on full strength MS medium showed highest leaf number (6.77±0.94). In vitro raised shoots were allowed to root on different strengths of MS medium fortified with IAA and IBA at different concentrations. Experimentally, 3.0 mg/L IBA was enabled to induce maximum rootlets number (10.0±9.82) on full strength MS medium. Afterwards, regenerated shoots with well developed roots were successfully subjected to hardening process and were acclimatized. The survived plantlets showed 66.67%survival frequency without any morphological abnormality. Conclusions: The results demonstrated that different explants were good source of callus induction, morphology analysis as well as indirect plantlets regeneration.

  6. Asymbiotic culture of Cattleya intermedia Graham (Orchidaceae: the influence of macronutrient salts and sucrose concentrations on survival and development of plantlets

    Directory of Open Access Journals (Sweden)

    Márcio Hisayuki Sasamori

    2015-09-01

    Full Text Available ABSTRACTCattleya intermediais an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium and macronutrient salts (complete Murashige and Skoog (MS medium and half MS medium (with half-strength macronutrients on survival and development of the plantlets was evaluated. In all treatments 100% plantlet survival was achieved. The integrated analysis of height of aerial part, number of leaves per plantlet, fresh mass, number of roots per plantlet and length of the longest root showed that the plantlets exhibited greatest development at the half-strength macronutrient concentrations with 45 or 60 g L-1 of sucrose, as well as at the complete macronutrient concentration with 60 g L-1 of sucrose. Plantlets acclimatized and reintroduced to an environment in which the species occurs naturally exhibited 98.6% survival. The results obtained in this study allowed the establishment of optimal conditions for asymbiotic micropropagation, which is a requisite for future studies focused on conservation of C. intermedia.

  7. Efficient Axillary Shoot Proliferation and in Vitro Rooting of Apple cv. ‘Topaz’

    Directory of Open Access Journals (Sweden)

    Snjezana KERESA

    2012-05-01

    Full Text Available ‘Topaz’ is a modern Czech apple cultivar well accepted by consumers and scab-resistant, providing reasons for the significant spreadof cv. ‘Topaz’ in European orchards, especially in the organic fruit production industry. Growing the apple trees on their own rootsprovides some advantages in comparison with grafted trees. Micropropagation is the method of choice for plantlet production for thispurpose as well as for the establishment of healthy mother stock trees as a source of scions. The efficiency of axillary shoot proliferationwas examined on four media differing in plant growth regulators and their concentrations, and from three explant types: intact ordecapitated and defoliated microshoots placed vertically and one-nodal segments placed horizontally. All media consisted of Quoirin andLepoivre (QL macroelements and Murashige and Skoog (MS microelements. Furthermore, rooting efficiency on six different media/treatments was analyzed. Media with 1 mg/L 6-benzylaminopurine (BA or BA (0.5 mg/L + 1.5 mg/L kinetin (Kin produced similarnumber of microshoots per inoculated one (2.5 and 2.4, respectively. Medium with 1 mg/L thidiazuron (TDZ produced significantlyhigher number of shoots (3.6 but they were fasciated. Three different explant types also produced similar numbers of microshoots.High rooting efficiency (68.7%, a high number of roots per shoot (6.6 and the best quality of shoots were obtained in rooting mediumcontaining 2 mg/L of indole-3-butyric acid (IBA. An efficient method of shoot proliferation was established, and, since rooting was themost critical step, an efficient procedure for rooting apple cv. ‘Topaz’ was established.

  8. An Efficient Agrobacterium-Mediated Transformation of Strawberry cv. Camarosa by a Dual Plasmid System

    Directory of Open Access Journals (Sweden)

    Fatemeh Haddadi

    2015-02-01

    Full Text Available An Agrobacterium-mediated transformation method was applied to introduce the luciferase reporter gene under the control of the CaMV35S promoter in the pGreen0049 binary vector into strawberry cv. Camarosa. The in vitro regeneration system of strawberry leaves to be used in the transformation was optimized using different TDZ concentrations in MS medium. TDZ at 16 µM showed the highest percentage (100% of shoot formation and the highest mean number of shoots (24 produced per explant. Studies on the effects of different antibiotics, namely timentin, cefotaxime, carbenicillin and ampicillin, on shoot regeneration of strawberry leaf explants showed the best shoot regeneration in the presence of 300 mg/L timentin and 150 mg/L cefotaxime. Assessment of the different factors affecting Agrobacterium mediated-transformation of strawberry with the luciferase gene showed the highest efficiency of putative transformant production (86% in the treatment with no preculture, bacterial OD600 of 0.6 and the addition of 150 mg/L cefotaxime in the pre-selection and selection media. The presence of the luciferase gene in the plant genome was verified by the luciferase reporter gene assay, nested PCR amplification and dot blot of genomic DNA isolated from the young leaves of each putatively transformed plantlet.

  9. An efficient Agrobacterium-mediated transformation of strawberry cv. Camarosa by a dual plasmid system.

    Science.gov (United States)

    Haddadi, Fatemeh; Aziz, Maheran Abd; Abdullah, Siti Nor Akmar; Tan, Soon Guan; Kamaladini, Hossein

    2015-02-23

    An Agrobacterium-mediated transformation method was applied to introduce the luciferase reporter gene under the control of the CaMV35S promoter in the pGreen0049 binary vector into strawberry cv. Camarosa. The in vitro regeneration system of strawberry leaves to be used in the transformation was optimized using different TDZ concentrations in MS medium. TDZ at 16 µM showed the highest percentage (100%) of shoot formation and the highest mean number of shoots (24) produced per explant. Studies on the effects of different antibiotics, namely timentin, cefotaxime, carbenicillin and ampicillin, on shoot regeneration of strawberry leaf explants showed the best shoot regeneration in the presence of 300 mg/L timentin and 150 mg/L cefotaxime. Assessment of the different factors affecting Agrobacterium mediated-transformation of strawberry with the luciferase gene showed the highest efficiency of putative transformant production (86%) in the treatment with no preculture, bacterial OD600 of 0.6 and the addition of 150 mg/L cefotaxime in the pre-selection and selection media. The presence of the luciferase gene in the plant genome was verified by the luciferase reporter gene assay, nested PCR amplification and dot blot of genomic DNA isolated from the young leaves of each putatively transformed plantlet.

  10. Suppression of maize plantlets growth under mutual action of chromium and nickel

    Directory of Open Access Journals (Sweden)

    О. М. Piskova

    2007-04-01

    Full Text Available Mutual toxicity of chromium and nickel sulphates for growth and development of maize plantlets was studied. Its combined action at low concentrations (10–5 M results to significant suppression of growth of both above-ground and root systems of maize. At the beginning of growth the greater negative effect of the salts mixture at high concentration (10–4 M was observed for the plantlets’ roots. Then, on the sixth day, the growth of above-ground and root system was inhibited equally. Application of high nickel concentration and low chrome concentration, in contrast to high chromium and low nickel levels, depresses the biomass gain. Meanwhile the length of roots and leaves was affected by both variants of concentrations identically.

  11. Asymbiotic germination, seedling development and plantlet propagation of Encyclia aff. oncidioides - an endangered orchid

    Directory of Open Access Journals (Sweden)

    Ewa Łojkowska

    2011-01-01

    Full Text Available In order to estimate the best germination conditions of Encyclia aff. oncidioides seeds, five different media (Fast, Knudson C modified by Vajrabhaya, Murashige and Skoog, PB2 and modified Vacin and Went with different concentrations of plant growth regulators such as benzyladenine (BA, naphthaleneacetic acid (NAA and gibberellic acid (GA3 were tested. No beneficial effect was observed when BA and NAA were applied to the germination medium and GA3 inhibited germination. The effect of light, activated charcoal, coconut water and casein hydrolysate on seed germination was also studied. The growth rate of seedlings on three different media supplemented with activated charcoal and plant growth regulators was checked. The applied plant growth regulators had no beneficial effect on the further growth of seedlings. Fast and PB2 media with 0.2% activated charcoal proved to be the best for E. aff. oncidioides seed germination, seedling development and plantlet propagation.

  12. Somatic embryogenesis and plantlet from petal cultures of pomegranate, Punica granatum L.

    Science.gov (United States)

    Nataraja, K; Neelambika, G K

    1996-07-01

    Somatic embryos were induced through petal cultures on MS supplemented with various growth adjuvants individually or in different combinations. Numerous embryoids formed from friable callus on MS with 5 mg/L of IAA, IBA or NAA developed only roots, but those formed on MS with 5 mg/L of BAP or KN developed shoots. Explants reared on MS supplemented with IAA (5 mg/L) and BAP (5 mg/L) though differentiated numerous embryoids but developed multilobed or fused cotyledons. However, callus induced on MS with IAA (1 mg/L) or IBA (1 mg/L) on subculturing in the same medium with half-strength salts and sucrose (4%) produced both roots and shoots resulting in plantlets.

  13. Asymptotically safe grand unification

    Science.gov (United States)

    Bajc, Borut; Sannino, Francesco

    2016-12-01

    Phenomenologically appealing supersymmetric grand unified theories have large gauge representations and thus are not asymptotically free. Their ultraviolet validity is limited by the appearance of a Landau pole well before the Planck scale. One could hope that these theories save themselves, before the inclusion of gravity, by generating an interacting ultraviolet fixed point, similar to the one recently discovered in non-supersymmetric gauge-Yukawa theories. Employing a-maximization, a-theorem, unitarity bounds, as well as positivity of other central charges we nonperturbatively rule out this possibility for a broad class of prime candidates of phenomenologically relevant supersymmetric grand unified theories. We also uncover candidates passing these tests, which have either exotic matter or contain one field decoupled from the superpotential. The latter class of theories contains a model with the minimal matter content required by phenomenology.

  14. Asymptotically Safe Grand Unification

    CERN Document Server

    Bajc, Borut

    2016-01-01

    Phenomenologically appealing supersymmetric grand unified theories have large gauge representations and thus are not asymptotically free. Their ultraviolet validity is limited by the appearance of a Landau pole well before the Planck scale. One could hope that these theories save themselves, before the inclusion of gravity, by generating an interacting ultraviolet fixed point, similar to the one recently discovered in non-supersymmetric gauge-Yukawa theories. Employing a-maximization, a-theorem, unitarity bounds, as well as positivity of other central charges we nonperturbatively rule out this possibility for a broad class of prime candidates of phenomenologically relevant supersymmetric grand unified theories. We also uncover candidates passing these tests, which have either exotic matter or contain one field decoupled from the superpotential. The latter class of theories contains a model with the minimal matter content required by phenomenology.

  15. Special grand unification

    Science.gov (United States)

    Yamatsu, Naoki

    2017-06-01

    We discuss new-type grand unified theories based on grand unified groups broken into their special subgroups, as well as their regular subgroups. In the framework, when we construct 4-dimensional (4D) chiral gauge theories, i.e., the Standard Model (SM), 4D gauge anomaly cancelation restricts the minimal number of generations of the 4D SM Weyl fermions. We show that in a 6-dimensional (6D) SU(16) gauge theory on M^4× T^2/\\mathbb{Z}_2, one generation of the SM fermions can be embedded into a 6D bulk Weyl fermion. For the model including 3 chiral generations of the SM fermions, the 6D and 4D gauge anomalies on the bulk and fixed points are canceled out without exotic 4D chiral fermions.

  16. LA GRANDE DESCENTE

    CERN Multimedia

    The first endcap disc of CMS being lowered slowly and carefully 100 m underground into the experimental cavern. The disc is one of 15 large pieces to make the grand descent.  The uniquely shaped slice, 16 m high, about 50 cm thick weighs 400 tonnes. The two HF that were lowered earlier in November can also be seen in the foreground and background.  

  17. Grand alliance HDTV

    Science.gov (United States)

    Petajan, Eric D.

    1995-12-01

    Terrestrial broadcast television in the United States has remained essentially unchanged in the last fifty years except for the addition of color and stereo sound. Today, personal computers are addressing the need for random access of high resolution images and CD quality audio. Furthermore, advances in digital video compression and digital communication technology have cleared the way toward offering high resolution video and audio services to consumers using traditional analog communications channels. In 1987, the U.S. Federal Communications Commission (FCC) chartered an advisory committee to recommend an advanced television system for the United States. From 1990 to 1992, the Advanced Television Test Center tested four all-digital systems, one analog High Definition Television (HDTV) system, and one enhancement NTSC system using broadcast and cable television environment simulators. The formation of the HDTV Grand Alliance in May of 1993 resulted from the withdrawal of the only analog HDTV system from the competition and a stalemate between the other four all- digital systems. The HDTV Grand Alliance system is composed of the best components from previously competing digital systems demonstrated to the FCC. Moving Pictures Experts Group (MPEG-2) syntax is used with novel encoding techniques to deliver a set of video scanning formats for a variety of applications. This paper describes the important features and concepts embodied in the HDTV Grand Alliance system.

  18. Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR).

    Science.gov (United States)

    Chaudhary, B; Kumar, S; Prasad, K V S K; Oinam, G S; Burma, P K; Pental, D

    2003-06-01

    In Agrobacterium-mediated genetic transformation of cotton (Gossypium hirsutum L. cv. Coker 310FR) the frequency at which somatic embryos were converted to plantlets was significantly improved by subjecting the embryos to slow physical desiccation. We used Agrobacterium strain GV3101 containing the binary vector pGSFR with the nos-nptII gene for in vitro selection and the 35S gus-int fragment as a reporter to optimize the transformation protocol. Although the concentration of kanamycin was reduced during embryogenesis and embryo maturation, even at the lower levels somatic embryos were predominantly abnormal, showing hypertrophy and reduced or fused cotyledons or poor radicle ends. A majority of these embryos (more than 75%) were beta-glucuronidase (GUS)-positive. Embryos with an abnormal appearance showed a very poor conversion to plantlets. However, these embryos, when subjected to slow physical desiccation followed by transfer to fresh medium, regenerated single or multiple shoots from the cotyledonary end. These shoots could be grafted on wild-type seedling stocks in vitro, which, following their transfer to soil, developed normally and set seeds. Regenerated plants tested positive for the transgene by Southern analysis. An overall scheme for the high-frequency production of cotton transgenics from both normal and abnormal appearing somatic embryos is presented.

  19. EIN3-like gene expression during fruit ripening of Cavendish banana (Musa acuminata cv. Grande naine).

    Science.gov (United States)

    Mbéguié-A-Mbéguié, Didier; Hubert, Olivier; Fils-Lycaon, Bernard; Chillet, Marc; Baurens, Franc-Christophe

    2008-06-01

    Ethylene signal transduction initiates with ethylene binding at receptor proteins and terminates in a transcription cascade involving the EIN3/EIL transcription factors. Here, we have isolated four cDNAs homologs of the Arabidopsis EIN3/EIN3-like gene, MA-EILs (Musa acuminata ethylene insensitive 3-like) from banana fruit. Sequence comparison with other banana EIL gene already registered in the database led us to conclude that, at this day, at least five different genes namely MA-EIL1, MA-EIL2/AB266318, MA-EIL3/AB266319, MA-EIL4/AB266320 and AB266321 exist in banana. Phylogenetic analyses included all banana EIL genes within a same cluster consisting of rice OsEILs, a monocotyledonous plant as banana. However, MA-EIL1, MA-EIL2/AB266318, MA-EIL4/AB266320 and AB266321 on one side, and MA-EIL3/AB266319 on the other side, belong to two distant subclusters. MA-EIL mRNAs were detected in all examined banana tissues but at lower level in peel than in pulp. According to tissues, MA-EIL genes were differentially regulated by ripening and ethylene in mature green fruit and wounding in old and young leaves. MA-EIL2/AB266318 was the unique ripening- and ethylene-induced gene; MA-EIL1, MA-EIL4/Ab266320 and AB266321 genes were downregulated, while MA-EIL3/AB266319 presented an unusual pattern of expression. Interestingly, a marked change was observed mainly in MA-EIL1 and MA-EIL3/Ab266319 mRNA accumulation concomitantly with changes in ethylene responsiveness of fruit. Upon wounding, the main effect was observed in MA-EIL4/AB266320 and AB266321 mRNA levels, which presented a markedly increase in both young and old leaves, respectively. Data presented in this study suggest the importance of a transcriptionally step control in the regulation of EIL genes during banana fruit ripening.

  20. OpenCV for secret agents

    CERN Document Server

    Howse, Joseph

    2015-01-01

    This book is for programmers who want to expand their skills by building fun, smart, and useful systems with OpenCV. The projects are ideal in helping you to think creatively about the uses of computer vision, natural user interfaces, and ubiquitous computers (in your home, car, and hand).

  1. Grand Challenge Problem 10

    DEFF Research Database (Denmark)

    Dirckinck-Holmfeld, Lone; Balacheff, Nicolas; Bottino, Rosa Maria

    2016-01-01

    settings across Europe. However, to strengthen the various research practices and to develop a common scientific language on TEL research the Grand Challenge Problem is to establish a vivid network and a community of practice among the research labs. TELEARC (Technology Enhanced Learning European Advanced......Small and medium sized research labs (SMLs) are dominating European TEL research. This is justified by the great numbers of countries and regions in Europe needing to develop a research and innovation competence to facilitate the diverse educational systems contextualized in various institutional...... Research Consortium) has been established to realize such a network. The chapter presents the framework of TELEARC....

  2. Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Denis Alcides-Rezende

    2008-01-01

    Full Text Available The objective of this work is to analyse the integration of information systems and information technology resources in the municipal planning of 14 small cities of Rio Grande do Sul (Brazil. The research methodology consisted of a multiple case study together with a convenient non-probabilistic sample chosen through a research protocol. The results demonstrate the difficulties of these cities to organise the municipal data as well as their struggle for accessibility of information and planning for management and control.

  3. Rhealba® oat plantlet extract: evidence of protein-free content and assessment of regulatory activity on immune inflammatory mediators.

    Science.gov (United States)

    Mandeau, Anne; Aries, Marie-Françoise; Boé, Jean-François; Brenk, Manuela; Crebassa-Trigueros, Véronique; Vaissière, Clémence; Teysseyre, Valérie; Bieber, Thomas

    2011-06-01

    Owing to their high content of flavonoids and saponins, plantlets of Avena sativa L. (Poaceae) are likely to possess anti-inflammatory and immunoregulatory properties of value in the treatment of atopic dermatitis (AD). With a view to its potential use in atopic subjects at risk of developing sensitisation to dietary proteins, we prepared a plantlet extract without proteins and isolated 2 flavonoids, isoorientin-2''- O-arabinoside (1) and isovitexin-2''- O-arabinoside (2), and two saponins, avenacosides A (3) and B (4). The absence of protein in this extract was evidenced by electrophoresis and Western immunoblotting. Furthermore, Western immunoblotting demonstrated the absence of cross-reaction between grain and plantlet proteins. We evaluated the anti-inflammatory activity of the plantlet extract and its compounds IN VITRO in a model of keratinocyte inflammation: 6-keto prostaglandin F1 α production was inhibited by the plantlet extract (- 35 % and - 57 % at 10 and 30 µg/mL, respectively; p isoorientin-2''- O-arabinoside (- 31 %, - 51 %, and - 56 % at 3, 10, and 30 µg/mL, respectively; p < 0.001). Intracellular interleukin-2 production in activated T lymphocytes was also inhibited by 16 %, 27 %, and 31 % with 3, 10, and 30 µg/mL plantlet extract, respectively, and by 23 % and 32 % with 3 and 10 µg/mL avenacoside A, respectively, (p < 0.001), demonstrating their immunoregulatory activity IN VITRO. The plantlet extract was also effective on the phenotype and function of dendritic cells (DC) differentiated from monocytes. It decreased the expression of major histocompatibility complex class II molecules on DC and significantly impaired their stimulatory activity on autologous T-cell proliferation (-25 %, p < 0.05). In conclusion, this protein-free oat plantlet extract exhibits anti-inflammatory and immunoregulatory activities in vitro.

  4. Establishment of multiple shoot clumps from maize(Zea mays L.) and regeneration of herbicide-resistant transgenic plantlets

    Institute of Scientific and Technical Information of China (English)

    李国圣; 张卿伟; 张举仁; 毕玉平; 单雷

    2002-01-01

    A kind of quick, efficient and season-free inducing embryoid and multiple shoot clumps system from shoot tip meristems that derived from elite inbreds of maize was established. The herbicide-resistant gene als(coding Acetolactate synthase) isolated from a mutant of Arabidopsis thaliana was transferred to tissue pieces of maize multiple shoot clumps by microprojectile bombardment. Herbicide-resistant tissue and regenerants were obtained through selections with herbicide chlorsulfuron. PCR analysis and Southern blot hybridization indicated that gene als has been transferred to some regenerants. The test of spraying chlorsulfuron displayed that the transgenic plantlets and R1 plants had favorable herbicide-resistant trait. We have established a new genotype-free system of maize which could rapidly and efficiently produce large quantities of transgenic plantlets.

  5. Evaluation of Streptomyces sp. strain g10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets.

    Science.gov (United States)

    Getha, K; Vikineswary, S; Wong, W H; Seki, T; Ward, A; Goodfellow, M

    2005-01-01

    Streptomyces sp. strain g10 exhibited strong antagonism towards Fusarium oxysporum f.sp. cubense (Foc) races 1, 2 and 4 in plate assays by producing extracellular antifungal metabolites. Treating the planting hole and roots of 4-week-old tissue-culture-derived 'Novaria' banana plantlets with strain g10 suspension (10(8) cfu/ml), significantly (P < 0.05) reduced wilt severity when the plantlets were inoculated with 10(4) spores/ml Foc race 4. The final disease severity index for leaf symptom (LSI) and rhizome discoloration (RDI) was reduced about 47 and 53%, respectively, in strain g10-treated plantlets compared to untreated plantlets. Reduction in disease incidence was not significant (P < 0.05) when plantlets were inoculated with a higher concentration (10(6) spores/ml) of Foc race 4. Rhizosphere population of strain g10 showed significant (P = 0.05) increase of more than 2-fold at the end of the 3rd week compared to the 2nd week after soil amendment with the antagonist. Although the level dropped, the rhizosphere population at the end of the 6th week was still nearly 2-fold higher than the level detected after 2 weeks. In contrast, the root-free population declined significantly (P = 0.05), nearly 4-fold after 6 weeks when compared to the level detected after 2 weeks. Neither growth-inhibiting nor growth-stimulating effects were observed in plantlets grown in strain g10-amended soil.

  6. Protocol for acclimatization of in vitro cultured Potamogeton praelongus – aspect of plantlet size and type of substrate

    Directory of Open Access Journals (Sweden)

    Romana Prausová

    2015-03-01

    Full Text Available The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy and starting size of plantlets gained through in vitro seed germination (0–3, 3.1–5,5.1–6, 6.1–10 cm on plant growth. Three parameters (fresh weight, length, and the number of leaves were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (≤6, 6.1–8, 8.1–10, 10.1–12, 12.1–15 cm. Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1–10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1–5 cm and 5.1–6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1–5 cm in the muddy substrate (more than 95% increase. The best overwintering results were gained in the 6.1–8 cm size category.

  7. Effect of Sodium Cyanide on Wheat (Triticum durum cv. Altar and T. aestivum cv. Cumhuriyet)

    DEFF Research Database (Denmark)

    Gemici, Meliha; Karshenass, A.M.; Tan, Kit

    2008-01-01

    The effect of sodium cyanide on the morphology of stem, leaves and grain yields of Triticum durum cv. Altar and Triticum aestivum cv. Cumhuriyet grown under glass was studied. Seeds were planted in six different sets of pots containing ordinary garden soil. After formation of the first leaves......, the first set was used as the control and watered using ordinary bottled water sold commercially. The other five sets with T. durum cv. Altar and T. aestivum cv. Cumhuriyet seedlings were additionally watered with various concentrations of sodium cyanide, the test-quantity used being 10-50 mg/L. Growth...... of individual plants was monitored until grain production. It was found that the sodium cyanide concentrations in the feed solutions affected plant stature, with the plants becoming progressively dwarfed with increasing dosage. Anomalies in the morphological and anatomical structure of the plant were also noted...

  8. The Limbic-Prefrontal Network Modulated by Electroacupuncture at CV4 and CV12

    Directory of Open Access Journals (Sweden)

    Jiliang Fang

    2012-01-01

    Full Text Available fMRI studies showed that acupuncture could induce hemodynamic changes in brain networks. Many of these studies focused on whether specific acupoints could activate specific brain regions and were often limited to manual acupuncture at acupoints on the limbs. In this fMRI study, we investigated acupuncture's modulation effects on brain functional networks by electroacupuncture (EA at acupoints on the midline of abdomen. Acupoints Guanyuan (CV4 and Zhongwan (CV12 were stimulated in 21 healthy volunteers. The needling sensations, brain activation, and functional connectivity were studied. We found that the limbic-prefrontal functional network was deactivated by EA at CV4 and CV12. More importantly, the local functional connectivity was significantly changed during EA stimulation, and the change persisted during the period after the stimulation. Although minor differences existed, both acupoints similarly modulated the limbic-prefrontal functional network, which is overlapped with the functional circuits associated with emotional and cognitive regulation.

  9. Induction of reactive oxygen species and the potential role of NADPH oxidase in hyperhydricity of garlic plantlets in vitro.

    Science.gov (United States)

    Tian, Jie; Cheng, Yaqi; Kong, Xiangyu; Liu, Min; Jiang, Fangling; Wu, Zhen

    2017-01-01

    Hyperhydricity is a physiological disorder associated with oxidative stress. Reactive oxygen species (ROS) generation in plants is initiated by various enzymatic sources, including plasma membrane-localized nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, cell wall-bound peroxidase (POD), and apoplastic polyamine oxidase (PAO). The origin of the oxidative burst associated with hyperhydricity remains unknown. To investigate the role of NADPH oxidases, POD, and PAO in ROS production and hyperhydricity, exogenous hydrogen peroxide (H2O2) and inhibitors of each ROS-producing enzyme were applied to explore the mechanism of oxidative stress induction in garlic plantlets in vitro. A concentration of 1.5 mM H2O2 increased endogenous ROS production and hyperhydricity occurrence and enhanced the activities of NADPH oxidases, POD, and PAO. During the entire treatment period, NADPH oxidase activity increased continuously, whereas POD and PAO activities exhibited a transient increase and subsequently declined. Histochemical and cytochemical visualization demonstrated that specific inhibitors of each enzyme effectively suppressed ROS accumulation. Moreover, superoxide anion generation, H2O2 content, and hyperhydric shoot frequency in H2O2-stressed plantlets decreased significantly. The NADPH oxidase inhibitor was the most effective at suppressing superoxide anion production. The results suggested that NADPH oxidases, POD, and PAO were responsible for endogenous ROS induction. NADPH oxidase activation might play a pivotal role in the oxidative burst in garlic plantlets in vitro during hyperhydricity.

  10. [Induction of hairy roots and plantlet regeneration of Bupleurum chinense DC].

    Science.gov (United States)

    Sun, Jing; Xu, Jie-sen; Zhao, Li-zi; Wei, Jian-he; Yang, Hong-yi; Sui, Chun

    2013-09-01

    In this study, the induction of hairy roots of Bupleurum chinense DC. was explored and established after experiments at different conditions: A. rhizogenes A4 was used to infect the leaves bases of B. chinense tube seedlings. The explants were co-cultured on Phytagel-solidified media for 3 days and then, were turned into solid media, similar with the co-culture media except that bacteriostat was added. After 10 days, rootlets began to appear and after 4 to 5 weeks, rootlets can be converted into liquid shaking culture stage. Plants regeneration from hairy root was useful for the research of new germplasm production and the variety improvement breeding. In the present study, the regenerated plants were obtained. One approach was to continuously culture under light conditions the seedlings which parting off spontaneously from the hairy roots during liquid shaking culture. The other approach was to culture the callus-like tissues produced by hairy roots with the optimized regeneration media for the induction of regenerated plants. The results of present study provide a technique to induce hairy roots and plantlet regeneration of B. chinense and this technique is helpful for the researches on metabolism, especially on the Agrobacterium-mediated genetic transformation of B. chinense.

  11. Micropropagation of an Exotic Ornamental Plant, Calathea crotalifera, for Production of High Quality Plantlets

    Directory of Open Access Journals (Sweden)

    Shahril Efzueni Rozali

    2014-01-01

    Full Text Available A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v NaOCl, 70% (v/v ethanol, and 0.3% (w/v HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP, 1.0 mg/L 1-Naphthaleneacetic acid (NAA, 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA compared to indolebutyric acid (IBA. The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

  12. Growing Tomato Plantlets on Various Mixtures of Peat and Sand or Peat and Perlite. Note 1

    Directory of Open Access Journals (Sweden)

    Antonia Patruno

    Full Text Available Given the considerable interest in use of substrates derived from various mixtures in the nursery sector and in light of the enormous variety of possibilities offered by this technique, in contrast with the still small number of researches dedicated to this theme, this study was set out to examine in-depth the growing of tomato plantlets on peat-based substrates. Two series of peat mixtures were produced, one with sand and the other with perlite, with a volume ratio of the other two components with respect to the peat of 1:0, 2.5:1, 1:1 and 1:2.5. Tomato seedlings were cultivated for 30 or 25 days in small perforated pots containing these mixtures. The irrigation was calculated by weighing each pot daily, measuring the water lost by evaporation-transpiration, then just past an established lower threshold value bringing the water back up to a defined upper threshold. Two water regimes were compared in the sand series and three in the perlite series.

  13. Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

    Science.gov (United States)

    Rozali, Shahril Efzueni; Rashid, Kamaludin A; Taha, Rosna Mat

    2014-01-01

    A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

  14. In vitro high frequency regeneration of plantlets of Vigna mungo and their ex vitro growth.

    Science.gov (United States)

    Agnihotri, S; Singh, R R; Chaturvedi, H C

    2001-09-01

    Of the five explants of V. mungo var. T9 used, the excised shoot tips gave best response with regard to offshoot formation followed by the embryonal axis explants. While a treatment comprising 0.5 mgL(-1) BAP, 0.5 mgL(-1) 2iP and 0.1 mgL(-1) NAA induced differentiation of an average 10 offshoots in shoot tip explants, only 3 offshoots were formed in the explants of embryonal axis in a treatment containing 0.5 mgL(-1) BAP and 0.1 mgL(-1) NAA, found optimum for them. Multiple shoots differentiated when explants with earlier regenerated and growing offshoots were first cultured in a treatment containing 0.1 mgL(-1) BAP, 0.25 mgL(-1) IAA and 5 mgL(-1) CCC and then subcultured in the same treatment but having only 1 mgL(-1) CCC. The isolated shoots rooted in 0.5 mgL(-1) IAA resulted in the formation of complete plantlets of an average height of 15 cm in 20 days. The in vitro-regenerated plants grew normally under field conditions and came to flowering as well.

  15. Effects of Lanthanum and Europium on Rooting of Plantlet Eriobotrya Japonica Lindl. in vitro

    Institute of Scientific and Technical Information of China (English)

    宋卫平; 洪法水; 万志刚; 周玉珍

    2002-01-01

    The effect of La3+ and Eu3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La3+ and Eu3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La3+ (1.0~3.0 μmol·L-1), Eu3+ (2.0~3.0 μmol*L-1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu3+.

  16. Iron oxide nanoparticles coated with β-cyclodextrin polluted of Zea mays plantlets

    Directory of Open Access Journals (Sweden)

    Mihaela Racuciu

    2012-01-01

    Full Text Available The present experimental investigation is focused on the study of assimilatory pigments and nucleic acid levels in young plants intended for agricultural use (Zea mays in presence of water based magnetic fluid added in culture medium. The magnetic fluid was constituted by coating the nanosized magnetic nanoparticles (with 10.55 nm average value of the physical diameter with β-cyclodextrin (C42H70O35 and further dispersion in water. After germination, various volume fractions (between 10 mL/L and 500 mL/L of the magnetic fluid was added daily in the culture medium of Zea mays plants still at their early ontogenetic stages. Toxicity symptoms leaded to brown spots covering the leaf surface for the highest magnetic fluid volume fractions used, a putative oxidative stress generated by iron excess treatment. Relatively small volume fraction of magnetic fluid solutions induced the increase of chlorophyll a level (up to 38%, the main photosynthesis pigment, as well that the nucleic acid level (up to 57% in Zea mays plantlets. All volume fractions of magnetic fluid solutions analyzed may have severe disruptive effects such as the ratio chlorophyll a/chlorophyll b (about 50% decreasing.

  17. Adição de torta de mamona em substratos na aclimatação de mudas micropropagadas de bananeira Effect of addition of castor bean pie in substrates in acclimatization of micropropagated banana plantlets

    Directory of Open Access Journals (Sweden)

    Adriana Novais Martins

    2011-03-01

    Full Text Available A utilização de mudas micropropagadas de bananeira que oferecem qualidade genética e fitossanitária, favorecendo o desenvolvimento, instalação e uniformidade do pomar, é importante para a exploração comercial da bananicultura. O objetivo deste trabalho foi avaliar a influência da adição da torta de mamona nos substratos na aclimatação de mudas micropropagadas de bananeira da cv. Willians. O delineamento estatístico utilizado foi o inteiramente casualizado, com 10 tratamentos, sendo 2 substratos e 5 dosagens de torta de mamona (0; 6; 12; 18 e 24 g vaso-1. O substrato Vivatto Slim Plus® possibilitou o melhor desenvolvimento das plantas na aclimatação. Não são recomendadas doses superiores a 12 g planta-1 de torta de mamona misturadas ao substrato na aclimatação de mudas de bananeira.The use of micropropagated banana tree seedlings offer genetic and phytosanitary qualities, favoring the development, installation and uniformity of the yield and it is very important for the commercial exploration of banana fruits. The objective of this study was to evaluate the influence of castor oil plant pie added to the substrate in the acclimatization of micropropagated banana plantlets cv. 'Willians'. It was use the completely randomized, with 10 treatments, being 2 substrates and 5 dosages of castor oil plant pie (0; 6; 12; 18 and 24 g for each recipient. The substrate Vivatto Slim Plus® showed the best development of the plants in acclimatization processes. Dosages above 12 g of castor oil plant pie for each plant are not recommended mixed to the substrate in the acclimatization of banana tree seedlings

  18. Grande Bretagne Hotel Athens

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Grande Bretagne酒店在过去的130年间被认为是雅典的“贵族包厢”。它坐落于市中心最著名的街区之一.对面是宪法广场,国会议院和国家花园,酒店的地理位置非常理想.通过短时间的步行就可以到达附近的高级购物区,餐馆,露天咖啡馆,博物馆和商务区,到目前为止,这家酒店已经接待了很多的国际领导人和知名人士。

  19. CV controls from design to operation

    CERN Document Server

    Blanc, D

    2002-01-01

    The cooling and Ventilation (CV) group has emphasised the need to redefine its organisational structure at the end of 98. The main objective of this operation was to ensure the CV group to be more competitive and efficient through the growing tasks of the LHC projects. The main evolution given to this reorganisation is that the new structure is more project oriented and then operates on three distinct axes: Design, Work and Operation. Process control project management requires a complete and early interaction and participation of all the actors involved. This procedure to be efficient and constructive must be considered and performed not only during the design stage but along the project planning phases and must go beyond the completion work including the process control operation activity. The paper explains the present project management for process control. It describes the present constraints and gives suggestions to a different approach to these projects to improve performances and efficiency of a contr...

  20. Earth, Moon, Sun, and CV Accretion Disks

    CERN Document Server

    Montgomery, M M

    2009-01-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting Cataclysmic Variable (CV) Dwarf Novae systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar and black hole systems. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our a...

  1. Chemical C-V Measurements on ZnO

    Science.gov (United States)

    Gabig, Sarah Jane; Farlow, Gary

    2008-10-01

    When metal/semiconductor schotky barriers are not practical, an electrolyte/semiconductor interface can be used to make capacitance-voltage (C-V) measurements. The physics of such electrochemical C-V measurements will be described. Electrical properties of ZnO were measured by electrochemical C-V techniques and photovoltage spectroscopy using an Accent 4400 Electrochemical CV system. Specifically, the electrical behavior of a 0.1 M ZnCl2 electrolyte-ZnO interface has been investigated with attention to the electrolyte-ZnO interface's C-V dependence on carrier frequency.

  2. Grand slam on cancer.

    Science.gov (United States)

    Gartrell, Nanette

    2014-01-01

    A winner of 59 Grand Slam championships including a record 9 Wimbledon singles titles, Martina Navratilova is the most successful woman tennis player of the modern era. Martina was inducted into the International Tennis Hall of Fame, named "Tour Player of the Year" seven times by the Women's Tennis Association, declared "Female Athlete of the Year" by the Associated Press, and ranked one of the "Top Forty Athletes of All-Time" by Sports Illustrated. Equally accomplished off the court, Martina is an author, philanthropist, TV commentator, and activist who has dedicated her life to educating people about prejudice and stereotypes. After coming out as a lesbian in 1981, Martina became a tireless advocate of equal rights for lesbian, gay, bisexual, and transgender (LGBT) people, and she has contributed generously to the LGBT community. Martina is the author of seven books, including most recently Shape Your Self: My 6-Step Diet and Fitness Plan to Achieve the Best Shape of your Life, an inspiring guide to healthy living and personal fitness. Martina was diagnosed with breast cancer in 2010.

  3. Warped Supersymmetric Grand Unification

    CERN Document Server

    Goldberger, W D; Smith, D R; Goldberger, Walter D.; Nomura, Yasunori; Smith, David R.

    2003-01-01

    We construct a realistic model of grand unification in AdS_5 truncated by branes, in which the unified gauge symmetry is broken by boundary conditions and the electroweak scale is generated by the AdS warp factor. We show that the model preserves the successful gauge coupling unification of the 4D MSSM at leading-logarithmic level. Kaluza-Klein towers, including those of XY gauge and colored Higgs multiplets, appear at the TeV scale, while the extra dimension provides natural mechanisms for doublet-triplet splitting and proton decay suppression. In one possible scenario supersymmetry is strongly broken on the TeV brane, in which case the lightest SU(3)_C x SU(2)_L x U(1)_Y gauginos are Dirac fermions, with universal masses at the weak scale, and the mass of the lightest XY gaugino is pushed well below that of the lowest gauge boson KK mode, improving the prospects for its production at the LHC. The bulk Lagrangian possesses a symmetry that we call GUT parity. If GUT parity is exact, the lightest GUT particle,...

  4. Direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots.

    Science.gov (United States)

    Razinger, Jaka; Lutz, Matthias; Schroers, Hans-Josef; Palmisano, Marilena; Wohler, Christian; Urek, Gregor; Grunder, Jürg

    2014-07-01

    A potential Delia radicum biological control strategy involving cauliflower plantlet inoculation with various fungi was investigated in a series of laboratory and glasshouse experiments. In addition to entomopathogenic fungi, fungi with a high rhizosphere competence and fungi with the ability to survive as saprotrophs in soil were tested. The following fungal species were evaluated in the experiments: Trichoderma atroviride, T. koningiopsis, T. gamsii, Beauveria bassiana, Metharhizium anisopliae, M. brunneum and Clonostachys solani. A commercial carbosulfan-based insecticide was used as a positive control. Additionally, two commercial products, one based on B. bassiana (Naturalis) and one on Bacillus thuringiensis (Delfin) were used as reference biocontrol agents. The aims were (i) to assess the pathogenicity of the selected fungal isolates to Delia radicum, (ii) to evaluate the fungal isolates' rhizosphere competence, with the emphasis on the persistence of the original inoculum on the growing roots, (iii) to assess possible endophytic plant tissue colonization, and (iv) to evaluate potential plant growth stimulating effects of the added inoculi. Significant pathogenicity of tested fungi against Delia radicum was confirmed in in vitro and glasshouse experiments. All tested fungi persisted on cauliflower rhizoplane. More importantly, the added fungi were found on thoroughly washed roots outside the original point of inoculation. This provided us with evidence that our tested fungi could be transferred via or grow with the elongating roots. In addition to colonizing the rhizoplane, some fungi were found inside the plant root or stem tissue, thus exhibiting endophytic characteristics. The importance of fungal ecology as a criterion in appropriate biological control agent selection is discussed. Copyright © 2014 Elsevier Inc. All rights reserved.

  5. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni)

    Science.gov (United States)

    Khalil, Shahid Akbar; Zamir, Roshan; Ahmad, Nisar

    2014-01-01

    Stevia rebaudiana (Bert.) is an emerging sugar alternative and anti-diabetic plant in Pakistan. That is why people did not know the exact time of propagation. The main objective of the present study was to establish feasible propagation methods for healthy biomass production. In the present study, seed germination, stem cuttings and micropropagation were investigated for higher productivity. Fresh seeds showed better germination (25.51–40%) but lost viability after a few days of storage. In order to improve the germination percentage, seeds were irradiated with 2.5, 5.0, 7.5 and 10 Gy gamma doses. But gamma irradiation did not show any significant change in seed germination. A great variation in survival of stem cutting was observed in each month of 2012. October and November were found the most suitable months for stem cutting survival (60%). In order to enhance survival, stem cuttings were also dipped in different plant growth regulators (PGRs) solution. Only indole butyric acid (IBA; 1000 ppm) treated cutting showed a higher survival (33%) than control (11.1%). Furthermore, simple and feasible indirect regeneration system was established from leaf explants. Best callus induction (84.6%) was observed on MS-medium augmented with 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D; 2.0 mg l−1). For the first time, we obtained the highest number of shoots (106) on a medium containing BA (1.5 mg l−1) and gibberellic acid (GA3; 0.5 mg l−1). Plantlets were successfully acclimatized in plastic pots. The current results preferred micropropagation (85%) over seed germination (25.51–40%) and stem cutting (60%). PMID:25473365

  6. Proteome characterization of cassava (Manihot esculenta Crantz somatic embryos, plantlets and tuberous roots

    Directory of Open Access Journals (Sweden)

    Rehman Samrina

    2010-02-01

    Full Text Available Abstract Background Proteomics is increasingly becoming an important tool for the study of many different aspects of plant functions, such as investigating the molecular processes underlying in plant physiology, development, differentiation and their interaction with the environments. To investigate the cassava (Manihot esculenta Crantz proteome, we extracted proteins from somatic embryos, plantlets and tuberous roots of cultivar SC8 and separated them by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE. Results Analysis by liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS yielded a total of 383 proteins including isoforms, classified into 14 functional groups. The majority of these were carbohydrate and energy metabolism associated proteins (27.2%, followed by those involved in protein biosynthesis (14.4%. Subsequent analysis has revealed that 54, 59, 74 and 102 identified proteins are unique to the somatic embryos, shoots, adventitious roots and tuberous roots, respectively. Some of these proteins may serve as signatures for the physiological and developmental stages of somatic embryos, shoots, adventitious roots and tuberous root. Western blotting results have shown high expression levels of Rubisco in shoots and its absence in the somatic embryos. In addition, high-level expression of α-tubulin was found in tuberous roots, and a low-level one in somatic embryos. This extensive study effectively provides a huge data set of dynamic protein-related information to better understand the molecular basis underlying cassava growth, development, and physiological functions. Conclusion This work paves the way towards a comprehensive, system-wide analysis of the cassava. Integration with transcriptomics, metabolomics and other large scale "-omics" data with systems biology approaches can open new avenues towards engineering cassava to enhance yields, improve nutritional value and overcome the

  7. Learning openCV computer vision with the openCV library

    CERN Document Server

    Bradski, Gary

    2008-01-01

    Learning OpenCV puts you right in the middle of the rapidly expanding field of computer vision. Written by the creators of OpenCV, the widely used free open-source library, this book introduces you to computer vision and demonstrates how you can quickly build applications that enable computers to see" and make decisions based on the data. With this book, any developer or hobbyist can get up and running with the framework quickly, whether it's to build simple or sophisticated vision applications

  8. Physiological responses of the hybrid larch (Larix × eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets.

    Science.gov (United States)

    Bonet, Amandine; Lelu-Walter, Marie-Anne; Faugeron, Céline; Gloaguen, Vincent; Saladin, Gaëlle

    2016-05-01

    Phytoextraction of Cd is a growing biotechnology although we currently know few Cd hyperaccumulators, i.e., plant species able to accumulate at least 0.1 mg Cd g(-1) dry weight in aerial organs. Owing their deep root system and high biomass, trees are more and more preferred to herbaceous species for phytoextraction. Assuming that conifers could be relevant models under cold climates, we investigated cadmium tolerance of the hybrid larch Larix × eurolepis Henry (Larix decidua × Larix kaempferi) and the efficiency of this species to store this metal. In vitro grown larches were chosen in order to reduce time of exposure and to more rapidly evaluate their potential efficiency to accumulate Cd. One-month-old plantlets were exposed for 2 and 4 weeks to 250 and 500 μM Cd. Results showed that they tolerated a 4-week exposure to 250 μM Cd, whereas the content of photosynthetic pigment strongly dropped in plantlets growing in the presence of 500 μM Cd. In the presence of 250 μM Cd, shoot growth slightly decreased but photosynthetic pigment and total soluble carbohydrate contents were not modified and no lipid peroxidation was detected. In addition, these plantlets accumulated proline, particularly in shoots (two to three times more than control). In roots, Cd concentration in the intracellular fraction was always higher than in the cell wall fraction contrary to shoots where Cd concentration in the cell wall fraction increased with time and Cd concentration in the medium. In shoots, Cd concentration was lower than in roots with a ratio of 0.2 after 4 weeks of exposure but stayed around 0.2 mg g(-1) dry weight, thus a value higher than the threshold requested for Cd hyperaccumulators. Hybrid larch would thus be a relevant candidate for field test of Cd phytoextraction.

  9. Cassini's Grand Finale

    Science.gov (United States)

    Spilker, L. J.; Edgington, S. G.; Altobelli, N.

    2016-12-01

    After more than 12 years in Saturn orbit, the Cassini-Huygens mission has entered its final year of data collection. Cassini will return its final bits of unique data on 15 September 2017 as it plunges into Saturn's atmosphere, vaporizing and satisfying planetary protection requirements. Since early 2016 Cassini's orbital inclination was slowly increased towards its final inclination. In November Cassini transitioned to a series of 20 orbits with peripases just outside Saturn's F ring that include some of the closest flybys of the tiny ring moons and excellent views of the F ring and outer A ring. Cassini's final close flyby of Titan will propel it across Saturn's main rings and into its final orbits. Cassini's Grand Finale begins in April 2017 and is comprised of 22 orbits at an inclination of 63 degrees. Cassini will repeatedly dive between the innermost ring and Saturn's upper atmosphere providing insights into fundamental questions unattainable during the rest of the mission. It will be the first spacecraft to explore this region. These close orbits provide the highest resolution observations of both the rings and Saturn, and direct in situ sampling of the ring particles' composition, plasma, Saturn's exosphere and the innermost radiation belts. Saturn's gravitational field will be measured to unprecedented accuracy, providing information on Saturn's interior structure and mass distribution in the rings. Probing the magnetic field will give insight into the nature of the magnetic dynamo and the true rotation rate of Saturn's interior. The ion and neutral mass spectrometer will sniff the exosphere and upper atmosphere and examine water-based molecules originating from the rings. The cosmic dust analyzer will sample particle composition from different parts of the main rings. Recent science highlights and science objectives from Cassini's final orbits will be discussed. This work was carried out in part at the Jet Propulsion Laboratory, California Institute of

  10. Retendering of ST-CV maintenance contract

    CERN Document Server

    Battistin, M

    2003-01-01

    During the next three years, the ST/CV group will be deeply involved in the installation works for the LHC project. During this period the need for maintenance activity will decrease. The minimum level will be reached during the “long shutdown” of the PS and SPS machines in 2005. The budget for the maintenance will decrease accordingly, thus the CV group had to review its maintenance strategy. The new contract, which started on January 1st 2003, has been defined to cope with these workload variations during the next years and to guarantee the minimum maintenance activity on the existing equipment. A lump-sum contract based on a win-win strategy has been discarded. The contractor no longer has to guarantee the performance of the CERN cooling and ventilation systems. A new price list strategy based on performance indicators and penalties has been chosen. The contractor now has to guarantee the performance of every maintenance operation demanded by CERN. This modification obliged the Operation section of the...

  11. Bureau d'études CV

    CERN Document Server

    Martel, C

    2005-01-01

    Le bureau d’études CV, au même titre que les autres bureaux d’études du CERN, est étroitement lié au déroulement du projet LHC. Dans ce contexte, l’adaptation devient une dimension essentielle pour assurer des prestations organisées et efficaces. En effet, la rentabilité, l’optimisation des ressources, la stratégie de sous-traitance, les contraintes imposées par la coordination générale LHC et la charge de travail diminuant vers la fin du projet, sont autant que facteurs qui influencent son organisation. L’analyse des expériences multiples et l’évaluation du risque organisationnel permettent de repositionner méthodiquement la structure face au changement, notamment pour l’horizon 2007. Le bureau d’études CV converge déjà vers une distribution « bimodale » : tandis que les tâches proches de la coordination restent confiées à un bureau interne réduit au strict minimum, une part grandissante des tâches est sous-traitée à des mandataires extérieurs.

  12. Effects of quantum flux density of photosynthesis and chloroplast ultrastructure in tissue-cultured plantlets and seedlings of Liquidambar styraciflua L. towards improved acclimatization and field survival

    Energy Technology Data Exchange (ETDEWEB)

    Lee, N.; Wetzstein, H.Y.; Sommer, H.E.

    1985-07-01

    Liquidambar styraciflua L. seedlings and tissue-cultured plantlets were grown under high, medium, or low quantum flux densities. Net photosynthesis, chlorophyll content, and chloroplast ultrastructure of leaves differentiated from these conditions were investigated. Seedling photosynthetic rates at light saturation were positively related to light pretreatments. Cultured plantlets under all light conditions had appreciably higher photosynthetic rates than noncultured seedlings. Chlorophyll in seedlings and plantlets was significantly higher in low light-treated plants. Seedling leaves had chloroplasts with abundant starch regardless of light pretreatment. In high light, starch granules were predominant and associated with disrupted granal structure. Low light seedling chloroplasts had smaller starch grains and well-formed grana. In contrast, tissue culture-differentiated leaves were devoid of starch; grana were well organized in higher quantum flux density treatments, but disorganized at low flux densities. 29 references, 7 figures, 1 table.

  13. 唐菖蒲试管苗叶片结构的研究%Research on the Leaf Structure of Gladiolus Plantlet and Field Seedling

    Institute of Scientific and Technical Information of China (English)

    唐蓉; 张继花; 韦梅芹; 龚维红

    2011-01-01

    [目的]为更好地了解试管苗生长情况,并对其生长进行调控.[方法]采用石蜡切片技术,对唐菖蒲试管苗和大田苗叶片结构进行观察.[结果]试管苗与大田苗叶片结构存在明显差异.试管苗叶片厚度、中脉厚度以及叶肉组织厚度与上下表皮厚度明显薄于大田生长的叶片,试管苗表皮细胞较小,排列也比较松散,但规则,其细胞间隙较大、叶片维管组织分化无大田生长叶片明显,叶片上气孔多,因此水分极易散失.[结论]该研究为更好地调控试管苗的生长提供了参考.%[Objective] The adjustment and control of gladiolus plantlet growth was better done through the understanding of its growth. [ Method] The leaf structure of gladiolus plantlet and field seedling was observed with paraffin section technology. [ Result] There was a obvious difference in the leaf structure between plantlet and field seedling. The thickness of leaf, vein, mesophyll tissue and between upper and lower epidermal of plantlet was significantly thinner than those of field leaf. The epidermal cell of plantlet was smaller, which arrangement was relatively loose, but regulate, with the larger gap among cells and non-significant differentiation of vascular tissue compared with that of field leaf, many more stomata in leaf resulting in easily water-losing. [ Conclusion] The reference for the study on the better adjustment and control plantlet growth was provided.

  14. Induction of embryogenic callus and plantlet regeneration from young leaves of high yielding mature oil palm

    Directory of Open Access Journals (Sweden)

    Yeedum, I.

    2004-09-01

    Full Text Available Callus induction and plantlet regeneration from young leaves of high-yielding mature oil palm were carried out using 10-year and 20-year-old trees from Thepa Research Station, Faculty of Natural Resources,Prince of Songkla University, Hat Yai, and Trang Agricultural College, respectively. Culture media used in this experiment were Murashige and Skoog (1962 and Oil Palm supplemented with various concentrations of α-naphthaleneacetic acid (NAA or 2,4- dichlorophenoxy acetic acid (2,4-D or dicamba (Di and antioxidants.Young leaves from 6th to 11st frond were excised, sterilized, cut into 5x5 mm pieces and cultured in the dark at 26±4ºC or 28±0.5ºC for 3 months. The results revealed that MS medium with 200 mg/l ascorbic acid (As and 1 mg/l Di (MS-AsDi gave the highest callus induction percentage (7.93 after culture for 3 months at 28±0.5ºC. Leaf segments from 6th - 8th frond yielded callus forming percentage at 10% (averaged from 1, 2.5 and 5 mg/l Di containing MS medium. Ascorbic acid as an antioxidant at concentration of 200 mg/l supplemented in MS medium in the presence of 2.5 mg/l Di produced the highest callus induction percentage (11.2 and number of nodules (7.06. A high percentage of embryogenic callus formation (66.67 was obtained when the calli were transferred to the same medium component supplemented with 0.5 mg/l Di and 1,000 mg/l casein hydrolysate (CH (MS-AsDiCH. Haustorial-staged embryos were observed to be isolated as an individual embryo and germinated on MS medium without plant growth regulator (MS-free. Development of root could be classified into two distinct types, fibrous and tap root.

  15. Application of DNA (RAPD and ultrastructure to detect the effect of cadmium stress in Egyptian clover and Sudan grass plantlets

    Directory of Open Access Journals (Sweden)

    Amina A. Aly

    2012-04-01

    Full Text Available BackgroundIn recent years, several plant species have been used as bioindicators to evaluate the toxicity of environmental contaminants on vegetal organisms. In this study, Egyptian clover and Sudan grass seedlings were grown in four cadmium (Cd concentration levels (0.0, 25, 50 and 100 µM in MS media to analyze growth responses, Cd accumulation in the shoots and roots of plantlets, proline contents, chlorophylls content and MDA levels of both plantlets. As well as RAPD analysis and leaves ultrastructure were detected.ResultsThe results showed that there was a significant decrease in root and shoot lengths, Chl a, Chl b, total Chl and carotenoids contents for both Egyptian clover and Sudan grass. However, there was a significant increase in Cd accumulation, proline and malondialdehyde (MDA levels. The genetic variation between Egyptian clover and Sudan grass were evaluated using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR markers to establish specific DNA markers associated with Cd stress. The results of transimssion electron microscopy (TEM showed a clear disorder in the Cd treated Egyptian clover and Sudan grass seedlings.ConclusionIn conclusion, biochemical, molecular and ultrastructure changes in Egyptian clover and Sudan grass could be used as a useful biomarker assay for the detection of genotoxic effects of Cd stress on plants. However, it is necessary to be further confirmed and optimized in the future research.

  16. MOLECULAR CONFIRMATION OF SEX IN REGENERATED PLANTLETS OF SPINE GOURD (Momordica dioica Roxb. Ex. WILD BY USING RAPD MARKERS

    Directory of Open Access Journals (Sweden)

    S. Raju

    2015-10-01

    Full Text Available Plant tissue culture techniques offer a great opportunity to overcome the limitations associated with the large scale cultivation of spine gourd. Present study was carried out to formulate the best possible media for large scale production of spine gourd and result of the study revealed that highest percentage (85% of embryogenic callus was obtained from MS medium supplemented with 2.0 mg/L each of 2, 4- Dichlorophenoxyacetic acid (2, 4-D and 6- Benzylamino Purine (BAP in leaf explants of spine gourd. Maximum number of shoots (12.15 ± 1.51 shoots were observed on MS medium augmented with BAP (4.0 mg/L in combination with L-glutamine (2.0 mg/L from leaf derived embryogenic callus of spine gourd. Identification of sex by using morphological characters in the newly regenerated plantlets of spine gourd at fourth leaf stage is another problem for large scale propagation of female plants. PCR based molecular marker OPA-15, a Random Amplified Polymorphic DNA (RAPD primer can be used as a differential marker to identify female plants form male plants at pre-flowering stage in newly regenerated plantlets (in vitro and as well as in field plants (in vivo of spine gourd. A unique amplification band (700 bp in size appeared only in female samples, but not in male samples of spine gourd.

  17. CO2, H2O exchange and stomatal regulation of regenerated Camptotheca acuminata plantlets during ex vitro acclimatization

    Institute of Scientific and Technical Information of China (English)

    WANG Hui-mei; LI Yan-hua; GAO Yin-xiang; ZU Yuan-gang

    2006-01-01

    For finding the changes in CO2, H2O exchange and their stomatal regulation during ex vitro acclimatization of regenerated Camptotheca acuminata plantlets, the net photosynthesis rate (Pn), respiration rate (Rd), light compensation point (Lc) and light saturation point (Ls), transpiration rate (Tr), stomatal conductance (gs) and water use efficiency(WUE) were measured during 37 days of ex vitro acclimatization. The results showed that Pn sharply increased until 29 days, then slightly decreased. A substantial decrease in Lc and a substantial increase of Ls in the former two weeks were observed, indicating the light regime enlargement for effective leaf photosynthesis. Tr and gs abruptly decreased during the first week then linearly increased until 29days ex vitro acclimatization, reflecting the strong regulation effect of stomata on water changes of ex vitro acclimating plantlets. Stomatal regulation effect on CO2 exchange was different from that on water exchange, i.e. Pn was almost independent of gs during the first week, while Pn was significantly correlated with gs thereafter (i.e. dual patterns). Different from dual patterns of gs-Pn relation, the Tr monotonously linearly increased with gs. Furthermore, WUE was almost independent on gs during the first week, while a marked decreasing tendency with gs was found thereafter. At the beginning of the acclimatization, WUE was mainly determined by photosynthetic capacity, while transpiration becomes a main determinant factor for WUE from 7 to 37 days' acclimatization.

  18. OPTIMIZATION OF YAM IN VITRO GENEBANKING: EFFECTS OF ACTIVATED CHARCOAL AND DARKNESS ON PLANTLETS OF THREE ACCESSIONS FROM BENIN

    Directory of Open Access Journals (Sweden)

    Agbidinoukoun Arnaud

    2013-12-01

    Full Text Available The aim of this study was to optimize the in vitro preservation of yam genetic resources through reduction of the number of subcultures. Effects of different concentrations of activated charcoal (1 g.l-1, 2 g.l-1and 3 g.l-1 and temporary darkness were tested on the in vitro morphogenesis of three beninese yam accessions (Dcr28, Dcr164 and Da93G1. Galzy glutamine was used as basis culture medium and explants were microcuttings obtained from four months old plantlets. The results indicated that the activated charcoal, alone or combined with temporary darkness has an inhibitory effect on the aerial organs formation but favors root development with a greater mean number of root shoots (9.3±1.67 with 3 g.l-1 of activated charcoal than the subtract without activated charcoal (2.5±0.17. A significant interaction was noted between accessions and concentration of activated charcoal indicating genotypic variability from the activated charcoal effect. The different accession plantlets growing in high concentration of activated charcoal culture media combined with temporary darkness were vigorous after eight month without subculture and subsequently allow doing one subculture per year

  19. Dichotic listening CV lateralization and developmental dyslexia.

    Science.gov (United States)

    Martínez, J A; Sánchez, E

    1999-08-01

    The present study was carried out on a sample of 125 right-handed boys who are described as follows: 50 boys with dyslexia, 50 controls of a similar age, and 25 controls according to reading level. Using an objective procedure based on regression, we selected three subgroups from among the poor readers: children with difficulties in the lexical pathway (surface dyslexics), children with difficulties in the sublexical pathway (phonological dyslexics), and children with problems in both pathways (mixed dyslexics). When the performances of these children on a dichotic listening task with CV syllables as stimuli were compared, it was found that although the children with dyslexia obtained lower lateralization indices than did the controls, the differences were only clearly significant when the most severe cases of phonological dyslexia were selected.

  20. Propagação de mudas de helicônia em biorreator de imersão temporária Propagation of heliconia plantlets in temporarily immersion bioreactor

    Directory of Open Access Journals (Sweden)

    Paulo Hercílio Viegas Rodrigues

    2006-01-01

    Full Text Available Novas técnicas de micropropagação têm sido estudadas visando à otimização da produção e redução dos custos de produção. Dentre elas, a técnica de micropropagação em biorreator de imersão temporária destaca-se pela utilização do meio de cultivo líquido em um sistema automatizado. Neste experimento avaliou-se, em três repetições, um biorreator artesanal de imersão temporária, utilizando explantes de Heliconia champneiana Griggs cv. Splash, submetidos a três subcultivos com quinze minutos de imersão em intervalos de uma hora (T1, quatro horas (T4, seis horas (T6 e oito horas (T8, em que foram avaliados o volume final de meio de cultivo, pH final, número de brotações, massa da matéria fresca, massa unitária e a funcionalidade do sistema. Resultados das avaliações entre os tratamentos de imersão temporária demonstraram que o melhor desempenho ocorreu no tratamento T1, sendo inviável o tratamento T8 para esse tipo de cultura. Na comparação com o método convencional (C, os tratamentos T1 e T4 foram superiores, demonstrando melhor eficiência da imersão temporária na produção de brotos. O protótipo do biorreator de imersão temporária teve bom desempenho, sendo funcional, de fácil manuseio e podendo ser utilizado no estudo de outras culturas.Novel micropropagation techniques have been studied aiming the optimization of production and cost reduction. Among these micropropagation using temporarily immersion in a bioreactor is a technique that uses liquid medium in an automatzed system. This research evaluated the efficacy of a handcraft temporarily immersion system, using explants of Heliconia champneiana Griggs cv. Splash. These were submitted to three subcultures with fifteen minutes of immersion in intervals of one hour (T1, four hours (T4, six hours (T6 and eight hours (T8. Weight of the fresh mass, number of plantlets, individual weight, final volume of culture media, pH final and the overall

  1. Business Strategy of CV Jaya Sampurna in Facing Soft Drink Distributor Competition in Bekasi

    Directory of Open Access Journals (Sweden)

    Seri Nurmala

    2015-11-01

    Full Text Available CV Jaya Sampurna is a soft drink distributor in Bekasi. Distributor company has a low profit margin, moreover the product sold is Fast Moving Consumers Goods with a very low profit margin (3.5%–5%. Therefore the company depends on a high volume of sales. Besides, there are many competitors in this industry, thus the business strategy is needed to improve the competitive advantage of the company. Given the conditions,this research was to identify the internal and the external factors, to identify the formulation of business strategy alternative, and to identify the recommendation of functional strategy for CV Jaya Sampurna. Research used descriptive method; and the type of research used was case study. Data were gathered from questionnaires and interview to three member of managers: Director, Operational Manager, and Finance Manager. Data wereanalyzed by using EFE, IFE, SWOT, IE, Grand Strategy Matrix and QSPM. The result of this research implies that the alternative strategy based on QSPM’s result was market penetration

  2. Protective effect of CV247 against cisplatin nephrotoxicity in rats.

    Science.gov (United States)

    Máthé, C; Szénási, G; Sebestény, A; Blázovics, A; Szentmihályi, K; Hamar, P; Albert, M

    2014-08-01

    CV247 (CV), an aqueous mixture of copper (Cu) and manganese (Mn) gluconates, vitamin C and sodium salicylate increased the antitumour effects of cisplatin (CDPP; cis-diamminedichloroplatinum) in vitro. We hypothesized that the antioxidant and cyclooxygenase-2 (COX-2; prostaglandin-endoperoxide synthase 2) inhibitory components of CV can protect the kidneys from CDPP nephrotoxicity in rats. CDPP (6.5 mg/kg, intraperitoneally) slightly elevated serum creatinine (Crea) and blood urea nitrogen (BUN) 12 days after treatment. Kidney histology demonstrated extensive tubular epithelial damage and COX-2 immunoreactivity increased 14 days after treatment. A large amount of platinum (Pt) accumulated in the kidney of CDPP-treated rats. Furthermore, CDPP decreased renal iron (Fe), molybdenum (Mo), zinc (Zn), Cu and Mn concentrations and increased plasma Fe and Cu concentrations. CDPP elevated plasma free radical concentration. Treatment with CV alone for 14 days (twice 3 ml/kg/day orally) did not influence these parameters. Chronic CV administration after CDPP reduced renal histological damage and slightly decreased COX-2 immunoreactivity, while failed to prevent the increase in Crea and BUN levels. Blood free radical concentration was reduced, that is, CV improved redox homeostasis. CV restored plasma Fe and renal Fe, Mo and Zn, while decreased Pt and elevated Cu and Mn concentrations in the kidney. Besides the known synergistic antitumour effects with CDPP, CV partially protected the kidneys from CDPP nephrotoxicity probably through its antioxidant effect.

  3. High frequency in vitro plantlet regeneration and antioxidant activity of Enicostema axillare(Lam. Raynal ssp. littoralis (Blume Raynal: An important medicinal plant

    Directory of Open Access Journals (Sweden)

    Kousalya Loganathan

    2014-09-01

    Conclusion: The present study not only enables reinforcement of wild plant populations using ex situ growth of individuals, but it also helps for high scale production of plantlets. A high correlation between antioxidant capacities and their total phenolic contents indicated that flavonoid compounds were a major contributor of antioxidant activity of both plants grown from nature and nodal culture.

  4. [Electroacupuncture at Guanyuan (CV 4) and Zhongwan (CV 12) modulates functional connectivity of the brain network in healthy volunteers].

    Science.gov (United States)

    Fang, Ji-liang; Hong, Yang; Wang, Xiao-ling; Liu, He-sheng; Wang, Yin; Liu, Jun; Wang, Lei; Xue, Chao; Zhou, Ke-hua; Song, Ming; Liu, Bao-yan; Zhu, Bing

    2011-10-01

    To observe the specific brain effects of electroacupuncture (EA) stimulation of Guanyuan (CV 4) and Zhongwan (CV 12). Twenty-one healthy volunteers were recruited in the present study. Two silver filiform needles were separately inserted into Guanyuan (OV 4) or Zhongwan (CV 12), and manipulated with uniform reducing-reinforcing method to induce "Deqi". fMRI scan was performed before needling, during needle retention, EA stimulation, and post-EA. Data of fMRI was analyzed by using software SPM 2. The volunteer subjective needling sensations were recorded. The activation, deactivation, short-distance and long-distance functional connectivity maps of different cerebral regions were analyzed by using whole brain correlation analysis. Comparison between the two acupoints showed that fullness feeling was stronger in CV 4 than in CV 12. EA at CV 4 and CV 12 induced a similar stronger and prevalent deactivation in the ventral medial prefrontal cortex and the anterior cingulated cortex (ACO). The deactivation of the ACC was stronger in the CV 4 group than in the CV 12 group. The default BOLD mode of the brain at rest was modified by needle retention and EA, respectively. The short-distance functional connection brain network was significantly changed after EA. Interestingly, the ventral medial prefrontal cortex and anteroinferior portion of the anterior cingulate cortex in the limbic-paralimbic-neocortical network (LPNN) were involved in the instant post-effects of EA. Relatively smaller differences in the brain functional activity and short-distance functional connectivity were found between these two acupoints. EA of CV 4 and CV 12 can modulate short-distance functional connectivity of the LPNN, and have fewer differences in inducing needling sensation and deactivation of ACC, etc.

  5. Facial Recognition using OpenCV

    Directory of Open Access Journals (Sweden)

    Valentin Petrut Suciu

    2012-03-01

    Full Text Available

    The growing interest in computer vision of the past decade. Fueled by the steady doubling rate of computing power every 13 months, face detection and recognition has transcended from an esoteric to a popular area of research in computer vision and one of the better and successful applications of image analysis and algorithm based understanding. Because of the intrinsic nature of the problem, computer vision is not only a computer science area of research, but also the object of neuro-scientific and psychological studies, mainly because of the general opinion that advances in computer image processing and understanding research will provide insights into how our brain work and vice versa.

    Because of general curiosity and interest in the matter, the author has proposed to create an application that would allow user access to a particular machine based on an in-depth analysis of a person’s facial features. This application will be developed using Intel’s open source computer vision project, OpenCV and Microsoft’s .NET framework.

  6. Facial Recognition using OpenCV

    Directory of Open Access Journals (Sweden)

    Shervin Emami

    2012-03-01

    Full Text Available The growing interest in computer vision of the past decade. Fueled by the steady doubling rate of computing power every 13 months, face detection and recognition has transcended from an esoteric to a popular area of research in computer vision and one of the better and successful applications of image analysis and algorithm based understanding. Because of the intrinsic nature of the problem, computer vision is not only a computer science area of research, but also the object of neuro-scientific and psychological studies, mainly because of the general opinion that advances in computer image processing and understanding research will provide insights into how our brain work and vice versa. Because of general curiosity and interest in the matter, the author has proposed to create an application that would allow user access to a particular machine based on an in-depth analysis of a person’s facial features. This application will be developed using Intel’s open source computer vision project, OpenCV and Microsoft’s .NET framework.

  7. Graphite whiskers in CV3 meteorites.

    Science.gov (United States)

    Fries, Marc; Steele, Andrew

    2008-04-04

    Graphite whiskers (GWs), an allotrope of carbon that has been proposed to occur in space, have been discovered in three CV-type carbonaceous chondrites via Raman imaging and electron microscopy. The GWs are associated with high-temperature calcium-aluminum inclusion (CAI) rims and interiors, with the rim of a dark inclusion, and within an inclusion inside an unusual chondrule that bears mineralogy and texture indicative of high-temperature processing. Current understanding of CAI formation places their condensation, and that of associated GWs, relatively close to the Sun and early in the condensation sequence of protoplanetary disk materials. If this is the case, then it is a possibility that GWs are expelled from any young solar system early in its history, thus populating interstellar space with diffuse GWs. Graphite whiskers have been postulated to play a role in the near-infrared (near-IR) dimming of type Ia supernovae, as well as in the thermalization of both the cosmic IR and microwave background and in galactic center dimming between 3 and 9 micrometers. Our observations, along with the further possibility that GWs could be manufactured during supernovae, suggest that GWs may have substantial effects in observational astronomy.

  8. Photoautotrophic Growth and Net Photosynthetic Rate of Sweet Potato Plantlets In Vitro as Affected by the Number of Air Exchanges of the Vessel and Type of Supporting Material

    Institute of Scientific and Technical Information of China (English)

    XIAO Yulan; Kozai Toyoki

    2006-01-01

    To produce high-quality sweet potato plantlets rapidly at low production costs, single nodal leafy cuttings of sweet potato (Ipomoea batatas (L.) Lam.) plantlets were cultured in vitro for 14 days on sugar- and phytohormone-free Murashige and Skoog (MS) liquid medium, at a photosynthetic photon flux (PPF) of 200 μmol·m-2·s-1 and a CO2 concentration (v/v) of 1.8×10-3. A factorial experiment was conducted with two levels of the number of air exchanges of the vessel (NAE), 8.7-12.2 h-1 and >12.2 h-1, and two types of supporting material, vermiculite and Florialite (a porous material). The control treatment consisted of a photomixotrophic culture using a sugar- and NAA-containing agar MS medium with an NAE of 2.4 h-1. PPF and CO2 concentrations were the same as the photoautotrophic treatments. In comparison with the control treatment, the photoautotrophic treatments with NAE of 8.7-12.2 h-1 (MF treatment) and ≥12.2 h-1 (HF treatment) were, respectively, 2.2 and 2.8 times in dry weight, and 3.7 and 4.2 times in net photosynthetic rate, when Florialite was used. The survival percentages of the plantlets in the field were, respectively, 86% and 97% in the MF and HF treatments, and 35% and 46% higher than that in the control treatment. The plantlets cultured with Florialite showed greater growth, compared with those cultured with vermiculite. Photoautotrophic micropropagation system with high NAE and the use of porous supporting material can produce high-quality plantlets and make it possible to reduce production costs.

  9. Proline Accumulation, Photosynthetic Abilities and Growth Characters of Sugarcane (Saccharum officinarum L.) Plantlets in Response to Iso-Osmotic Salt and Water-Deficit Stress

    Institute of Scientific and Technical Information of China (English)

    Suriyan Cha-um; Chalennpol Kirdmanee

    2009-01-01

    The aim of this study was to investigate the biochemical, physiological and morphological responses of sugarcane to iso-osmotic salt and water-deficit stress. Disease-free sugarcane plantlets derived from meristem cuttings were photo-autotrophically grown in MS media and subsequently exposed to-0.23 (control), -0.67 or -1.20 Mpa iso-osmotic NaCI (salt stress) or mannitol (water-deficit stress). Chlorophyll a (Chl a), chlorophyll b (Chl b), total carotenoids (Cx+c), maximum quantum yield of PSⅡ(Fv/Fm), photon yield of PSⅡ(φPSⅡ), stomatal conductance(Gs)and transpiration rate(E)in the stressed plantlets were significantly reduced when compared to those of plantlets of the control group (without mannitol or NaCI), leading to net-photosynthetic rate (Pn) and growth reduction with positive correlation. In addition, physiological changes and growth parameters of plantlets in the salt stress conditions were more sharply reduced than those in water-deficit stress conditions. On the other hand, the proline content and non-photochemical quenching (NPQ) in the leaves of stressed plantlets increased significantly, especially in response to iso-osmotic salt stress. The chlorophyll pigments in iso-osmotic stressed leaves were significantly degraded (r2=0.93), related to low water oxidation (r2=0.87), low net-photosynthetic rate (r2=0.81), and growth reduction (r2=0.97). The multivariate biochemical, physiological and growth parameters in the present study should be further used to develop salt, or drought, tolerance indices in sugarcane breeding programs.

  10. A novel combination of plant growth regulators for in vitro regeneration of complete plantlets of guar [Cyamopsis tetragonoloba (L.) Taub].

    Science.gov (United States)

    Verma, S; Gill, K S; Pruthi, V; Dhugga, K S; Randhaw, G S

    2013-12-01

    A novel combination of plant growth regulators comprising indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) and gibberellic acid (GA3) in Murashige and Skoog basal medium has been formulated for in vitro induction of both shoot and root in one culture using cotyledonary node explants of guar, (Cyamopsis tetragonoloba). Highest percentages of shoot (92%) and root (80%) induction were obtained in the medium containing (mg/L) 2 IBA, 3 BA and 1 GA3. Shoot regeneration from the cotyledonary node explants was observed after 10-15 days. Regeneration of roots from these shoots occurred after 20 to 25 days. The regenerated plantlets showed successful acclimatization on transfer to soil. This protocol is expected to be helpful in carrying out various in vitro manipulations in this economically and industrially important legume.

  11. High frequency embryoid and plantlet formation from tissue cultures of the Finger millet-Eleusine coracana (L.) Gaertn.

    Science.gov (United States)

    Sivadas, P; Kothari, S L; Chandra, N

    1990-07-01

    Compact nodulated embryogenic callus differentiated from cultured seeds of Eleusine coracana (Finger Millet) on Murashige and Skoog (1962) basal medium with 2,4-dichlorophenoxyacetic acid (1.0, 3.0 mg (l)). This embryogenic callus was maintained on a medium with a lower level of 2,4 - dichlorophenoxyacetic acid. At every subculture the embryogenic callus had some preexisting embryoids in it. With this method of subculture the callus has retained its morphogenic potential for four years. Following transfer to media with different levels of auxins and cytokinins, the callus showed varied patterns of growth and morphogenesis. Embryoids could be germinated in profusion to form plantlets which could be transferred to the field. Shoot buds also differentiated from the whole surface of the embryoid or from the flattened meristemoids.

  12. High-frequency in vitro plantlet regeneration from apical bud as a novel explant of Carum copticum L.

    Institute of Scientific and Technical Information of China (English)

    Mansoureh Salehi; Bahman Hosseini; Zohreh Jabbarzadeh

    2014-01-01

    Objective: To develop an in vitro regeneration system to increase the recovery of Carum copticum L. plantlets as a part of developing a metabolic engineering program.Methods:3-acetic acid and indole butyric acid on direct shoot regeneration and rooting of ajowan from apical bud explants were assessed. All explants were cultured on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzyl amino purine (BAP) (0, 2.2, 4.4, 8.8µ The efficacy of different concentrations and combinations of 6-benzyladenine, indole-Results: The maximum shoot regeneration frequency (97.5%) and the highest number of shoots produced from apical buds (34 shoots per explant) were obtained on MS medium fortified with BAP (4.4 µmol/L) and IAA (0.5 µmol/L). Low shoot regeneration frequency was observed in BAP free treatments. The effects of different strengths of MS medium and various concentrations of IAA and indole-3- butyric acid on rooting rate, length and average number of roots were also investigated. Application of indole-3- butyric acid (6 µmol/L) in full-strength MS medium, was more effective than IAA and resulted in highest shoot regeneration frequency with the rooting rate of 100% and highest mean number of roots per shoot (41.8). The rooted plantlets were acclimatized successfully in greenhouse conditions with a survival rate of 90%. mol/L) and indole-3-acetic acid (IAA) (0, 0.5, 1.1, 2.2 µmol/L). Conclusion: In this study, a simple and reliable regeneration and acclimatization protocol for Carum copticum has been presented. This protocol can be found very advantageous for a variety of purposes, including mass multiplication of Carum species, medicinal plant breeding studies and transgenic plant production.

  13. LEAF TOTAL NITROGEN CONCENTRATION AS AN INDICATOR OF NITROGEN STATUS FOR PLANTLETS AND YOUNG PLANTS OF EUCALYPTUS CLONES

    Directory of Open Access Journals (Sweden)

    Eric Victor de Oliveira Ferreira

    2015-08-01

    Full Text Available The use of leaf total nitrogen concentration as an indicator for nutritional diagnosis has some limitations. The objective of this study was to determine the reliability of total N concentration as an indicator of N status for eucalyptus clones, and to compare it with alternative indicators. A greenhouse experiment was carried out in a randomized complete block design in a 2 × 6 factorial arrangement with plantlets of two eucalyptus clones (140 days old and six levels of N in the nutrient solution. In addition, a field experiment was carried out in a completely randomized design in a 2 × 2 × 2 × 3 factorial arrangement, consisting of two seasons, two regions, two young clones (approximately two years old, and three positions of crown leaf sampling. The field areas (regions had contrasting soil physical and chemical properties, and their soil contents for total N, NH+4-N, and NO−3-N were determined in five soil layers, up to a depth of 1.0 m. We evaluated the following indicators of plant N status in roots and leaves: contents of total N, NH+4-N, NO−3-N, and chlorophyll; N/P ratio; and chlorophyll meter readings on the leaves. Ammonium (root and NO−3-N (root and leaf efficiently predicted N requirements for eucalyptus plantlets in the greenhouse. Similarly, leaf N/P, chlorophyll values, and chlorophyll meter readings provided good results in the greenhouse. However, leaf N/P did not reflect the soil N status, and the use of the chlorophyll meter could not be generalized for different genotypes. Leaf total N concentration is not an ideal indicator, but it and the chlorophyll levels best represent the soil N status for young eucalyptus clones under field conditions.

  14. Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa

    Science.gov (United States)

    Anjum, Sumaira; Abbasi, Bilal Haider

    2016-01-01

    In vitro-derived cultures of plants offer a great potential for rapid biosynthesis of chemical-free antimicrobial silver nanoparticles (AgNPs) by enhancing their phytochemical reducing potential. Here, we developed an efficient protocol for in vitro micropropagation of a high-value endangered medicinal plant species, Phlomis bracteosa, in order to explore its biogenic potential in biomimetic synthesis of antimicrobial AgNPs. Murashige and Skoog medium supplemented with 2.0 mg/L thidiazuron was found to be more efficient in inducing optimum in vitro shoot regeneration (78%±4.09%), and 2.0 mg/L indole-3-butyric acid was used for maximum root induction (86%±4.457%). Antimicrobial AgNPs were successfully synthesized by using aqueous extract (rich in total phenolics and flavonoids content) of in vitro derived plantlets of P. bracteosa. Ultraviolet–visible spectroscopy of synthesized AgNPs showed characteristic surface plasmon band in the range of 420–429 nm. The crystallinity, size, and shape of the AgNPs were characterized by X-ray diffraction and scanning electron microscopy. Face-centered cubic AgNPs of almost uniform spherical size (22.41 nm) were synthesized within a short time (1 hour) at room temperature. Fourier-transform infrared spectroscopy revealed that the polyphenols were mainly responsible for reduction and capping of synthesized AgNPs. Energy dispersive X-ray analysis further endorsed the presence of elemental silver in synthesized AgNPs. These biosynthesized AgNPs displayed significantly higher bactericidal activity against multiple drug-resistant human pathogens. The present work highlighted the potent role of in vitro-derived plantlets of P. bracteosa for feasible biosynthesis of antimicrobial AgNPs, which can be used as nanomedicines in many biomedical applications. PMID:27217745

  15. 78 FR 11678 - Notice of Inventory Completion: Grand Rapids Public Museum, Grand Rapids, MI

    Science.gov (United States)

    2013-02-19

    ... National Park Service Notice of Inventory Completion: Grand Rapids Public Museum, Grand Rapids, MI AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Grand Rapids Public Museum has completed an... Grand Rapids Public Museum. Repatriation of the human remains and associated funerary objects to...

  16. A practical introduction to computer vision with OpenCV

    CERN Document Server

    Dawson-Howe, Kenneth

    2014-01-01

    Explains the theory behind basic computer vision and provides a bridge from the theory to practical implementation using the industry standard OpenCV libraries Computer Vision is a rapidly expanding area and it is becoming progressively easier for developers to make use of this field due to the ready availability of high quality libraries (such as OpenCV 2).  This text is intended to facilitate the practical use of computer vision with the goal being to bridge the gap between the theory and the practical implementation of computer vision. The book will explain how to use the relevant OpenCV

  17. Reduced and unstratified crust in CV chondrite parent body.

    Science.gov (United States)

    Ganino, Clément; Libourel, Guy

    2017-08-15

    Early Solar System planetesimal thermal models predict the heating of the chondritic protolith and the preservation of a chondritic crust on differentiated parent bodies. Petrological and geochemical analyses of chondrites have suggested that secondary alteration phases formed at low temperatures (hydrothermal fluid. Putative 'onion shell' structures are not anymore a requirement for the CV parent body crust.Meteorites may unlock the history of the early solar system. Here, the authors find, through Ca-Fe-rich secondary phases, that the distinction between reduced and oxidized CV chondrites is invalid; therefore, CV3 chondrites are asteroid fragments that percolated heterogeneously via porous flow of hydrothermal fluid.

  18. Results from KASCADE-Grande

    Energy Technology Data Exchange (ETDEWEB)

    Bertaina, M., E-mail: bertaina@to.infn.it [Dipartimento di Fisica Generale dell' Universita, Torino (Italy); Apel, W.D. [Institut fuer Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Arteaga-Velazquez, J.C. [Universidad Michoacana, Instituto de Fisica y Matematicas, Morelia (Mexico); Bekk, K. [Institut fuer Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Bluemer, J. [Institut fuer Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Institut fuer Experimentelle Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Bozdog, H. [Institut fuer Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); Brancus, I.M. [National Institute of Physics and Nuclear Engineering, Bucharest (Romania); Buchholz, P. [Fachbereich Physik, Universitaet Siegen (Germany); Cantoni, E. [Dipartimento di Fisica Generale dell' Universita, Torino (Italy); Istituto di Fisica dello Spazio Interplanetario, INAF Torino (Italy); Chiavassa, A. [Dipartimento di Fisica Generale dell' Universita, Torino (Italy); Cossavella, F. [Institut fuer Experimentelle Kernphysik, KIT - Karlsruher Institut fuer Technologie (Germany); and others

    2012-11-11

    The KASCADE-Grande experiment, located at Karlsruhe Institute of Technology (Germany) is a multi-component extensive air-shower experiment devoted to the study of cosmic rays and their interactions at primary energies 10{sup 14}-10{sup 18} eV. Main goals of the experiment are the measurement of the all-particle energy spectrum and mass composition in the 10{sup 16}-10{sup 18} eV range by sampling charged (N{sub ch}) and muon (N{sub {mu}}) components of the air shower. The method to derive the energy spectrum and its uncertainties, as well as the implications of the obtained result, is discussed. An overview of the analyses performed by KASCADE-Grande to derive the mass composition of the measured high-energy comic rays is presented as well.

  19. The KASCADE-grande experiment

    Energy Technology Data Exchange (ETDEWEB)

    Bluemer, J; Apel, W D; Badea, F; Bekk, K; Bozdog, H; Daumiller, K; Doll, P; Engel, R; Engler, J [Institut fuer Kernphysik, Forschungszentrum Karlsruhe (Germany); Arteaga, J C; Cossavella, F; Souza, V de; Finger, M [Institut fuer Experimentelle Kernphysik, Universitaet Karlsruhe (Germany); Bertaina, M; Chiavassa, A; Cantoni, E; Di Pierro, F [Dipartimento di Fisica Generale dell' Universita Torino (Italy); Brancus, I M [National Institute of Physics and Nuclear Engineering, Bucharest (Romania); Brueggemann, M; Buchholz, P [Fachbereich Physik, Universitaet Siegen (Germany)], E-mail: Johannes.Bluemer@ik.fzk.de (and others)

    2008-07-15

    KASCADE-Grande is an extensive air shower array co-located with the original KASCADE air shower experiment at Forschungszentrum Karlsruhe, Germany. The multi-detector system allows to investigate the energy spectrum, composition, and anisotropies of cosmic rays in the energy range extended up to 10{sup 18} eV. An overview on the performance of the apparatus and first results are presented.

  20. Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Charlote Wink

    2007-01-01

    Full Text Available The occurrence of Cryptophlebia carpophagoides Clarke, 1951, (Lepidoptera: Olethreutidae, in fruit of Enterolobium contortisiliquum (Vell. Morong, has bun first registered in the state of Rio Grande do Sul, Brazil. The damaged fruit was collected in the campus of National University of Santa Maria, Santa Maria, from May to June of 2003 and 2004. It was evidenced that the caterpillars damage the fruit of that specie affecting the integrety, the vigor and the germination of the seeds.

  1. Grand Unification and Exotic Fermions

    CERN Document Server

    Feger, Robert P

    2015-01-01

    We exploit the recently developed software package LieART to show that SU(N) grand unified theories with chiral fermions in mixed tensor irreducible representations can lead to standard model chiral fermions without additional light exotic chiral fermions, i.e., only standard model fermions are light in these models. Results are tabulated which may be of use to model builders in the future. An SU(6) toy model is given and model searches are discussed.

  2. The KASCADE-Grande experiment

    Energy Technology Data Exchange (ETDEWEB)

    Apel, W.D. [Karlsruhe Institute of Technology - Campus North, Institut fuer Kernphysik, Karlsruhe (Germany); Arteaga, J.C. [Karlsruhe Institute of Technology - Campus South, Institut fuer Experimentelle Kernphysik, Karlsruhe (Germany); Badea, A.F.; Bekk, K. [Karlsruhe Institute of Technology - Campus North, Institut fuer Kernphysik, Karlsruhe (Germany); Bertaina, M. [Dipartimento di Fisica Generale dell' Universita Torino (Italy); Bluemer, J. [Karlsruhe Institute of Technology - Campus North, Institut fuer Kernphysik, Karlsruhe (Germany); Karlsruhe Institute of Technology - Campus South, Institut fuer Experimentelle Kernphysik, Karlsruhe (Germany); Bozdog, H. [Karlsruhe Institute of Technology - Campus North, Institut fuer Kernphysik, Karlsruhe (Germany); Brancus, I.M. [National Institute of Physics and Nuclear Engineering, Bucharest (Romania); Buchholz, P. [Universitaet Siegen, Fachbereich Physik, Siegen (Germany); Cantoni, E. [Dipartimento di Fisica Generale dell' Universita Torino (Italy); Istituto di Fisica dello Spazio Interplanetario, INAF Torino (Italy); Chiavassa, A. [Dipartimento di Fisica Generale dell' Universita Torino (Italy); Cossavella, F. [Karlsruhe Institute of Technology - Campus South, Institut fuer Experimentelle Kernphysik, Karlsruhe (Germany); Daumiller, K. [Karlsruhe Institute of Technology - Campus North, Institut fuer Kernphysik, Karlsruhe (Germany); Souza, V. de [Karlsruhe Institute of Technology - Campus South, Institut fuer Experimentelle Kernphysik, Karlsruhe (Germany); Di Pierro, F., E-mail: dipierr@to.infn.i [Dipartimento di Fisica Generale dell' Universita Torino (Italy); Doll, P.; Engel, R.; Engler, J.; Finger, M. [Karlsruhe Institute of Technology - Campus North, Institut fuer Kernphysik, Karlsruhe (Germany); Fuhrmann, D. [Bergische Universitaet Wuppertal, Fachbereich Physik, Wuppertal (Germany)

    2010-08-21

    KASCADE-Grande is the enlargement of the KASCADE extensive air shower detector, realized to expand the cosmic ray studies from the previous 10{sup 14}-10{sup 17} eV primary energy range to 10{sup 18} eV. This is performed by extending the area covered by the KASCADE electromagnetic array from 200x200 to 700x700 m{sup 2} by means of 37 scintillator detector stations of 10 m{sup 2} area each. This new array is named Grande and provides measurements of the all-charged particle component of extensive air showers (N{sub ch}), while the original KASCADE array particularly provides information on the muon content (N{sub {mu}}). Additional dense compact detector set-ups being sensitive to energetic hadrons and muons are used for data consistency checks and calibration purposes. The performance of the Grande array and its integration into the entire experimental complex is discussed. It is demonstrated that the overall observable resolutions are adequate to meet the physical requirements of the measurements, i.e. primary energy spectrum and elemental composition studies in the primary cosmic ray energy range of 10{sup 16}-10{sup 18} eV.

  3. Micropropagação de abacaxizeiro cv. Emepa 1 Micropropagation of pineapple, cv. Emepa 1

    Directory of Open Access Journals (Sweden)

    Ailton M. de Moraes

    2010-09-01

    Full Text Available Realizou-se esse trabalho com o objetivo de desenvolver um protocolo de micropropagação para o abacaxizeiro cv. Emepa 1, contemplando as fases de estabelecimento de explantes (EE, multiplicação (UM e enraizamento (EN. Como explantes se utilizaram gemas axilares, desinfestadas e inoculadas em meio MS sólido, contendo diferentes concentrações e combinações de 6-benzilaminopurina (BAP e de ácido naftalenoacético (ANA. Todas as culturas foram mantidas em sala de crescimento com temperatura de 25 ± 5 °C e fotoperíodo de 16 h luz, a uma intensidade luminosa de 30 µmol m-2 s-1. O delineamento experimental utilizado foi inteiramente casualizado, com seis tratamentos na fase EE, oito na fase MU e quatro na fase EN, dez repetições, sendo a unidade experimental constituída de um frasco contendo um broto. Concluiu-se que o estabelecimento de gemas axilares desta variedade de abacaxizeiro, pode ser realizado em meio de cultivo MS sem a adição de reguladores de crescimento; a multiplicação, em meio de cultura MS suplementado com 2,0 mg L-1 de BAP + 0,5 mg L-1 de ANA, enquanto a adição do ANA promove o enraizamento dos brotos.This work aimed to develop a micropropagation protocol of pineapple cv. Emepa 1. The cv. Emepa 1 axillary gems used were disinfested and inoculated in half MS solid with 5.8 pH. There was incubation in a growth room with temperature of 25 ± 5 °C and photoperiod of 16 h light at a luminous intensity of 30 mmol m-2 s-1. The cv. Emepa 1 micropropagation protocol was developed according to the existing literature, comprising the following phases: establishing of explants (EE; multiplication (MU; extent rooting (EN. A completely randomized design (CRD was used in all the phases as follows: EE - DIC with 6 treatments comprised of 10 repetitions containing 1 explants per bottle; MU - CRD with 8 treatments, comprised of 10 repetitions containing 1 explants per bottle. It was concluded that the concentration of 2% of

  4. Crescimento de mudas micropropagadas de bananeira micorrizadas em diferentes recipientes Growth of mycorrhizal micropropagated banana plantlets in different recipients

    Directory of Open Access Journals (Sweden)

    Patrícia Lopes Leal

    2005-04-01

    Full Text Available Realizou-se um experimento em casa de vegetação, com o objetivo de avaliar a utilização de diferentes recipientes e a eficiência da inoculação com fungo micorrízico arbuscular (FMA, Glomus clarum, no crescimento de mudas micropropagadas de bananeira. O delineamento experimental foi o de blocos casualizados, no esquema fatorial 2x2, sendo 2 tratamentos microbiológicos: Glomus clarum e controle; e 2 recipientes: blocos prensados e tubetes, com 6 repetições. O substrato utilizado para a confecção dos blocos e para o enchimento dos tubetes foi constituído por uma mistura de materiais orgânicos (bagaço de cana + torta de filtro de usina açucareira e vermiculita. Mudas de bananeira produzidas em blocos prensados e inoculadas com o FMA apresentaram melhores resultados quando comparadas com as produzidas nos tubetes, com incrementos na altura, na produção de matéria seca da parte aérea e no acúmulo de N, P e K de 90%, 829%, 2774%, 249% e 403%, respectivamente.An experiment under greenhouse was carried out in order to evaluate different recipients and the inoculation with arbuscular mycorrhizal fungus (AMF, Glomus clarum, on the growth of micropropagated banana plantlets. A random block design in a factorial scheme 2x2 (two microbiological treatments: Glomus clarum and control; two types of recipients: pressed blocks and plastic tubes, with six repetitions, was used. The substrate utilized to construct the pressed blocks and to fill the tubes was constituted by a mixture of organic residue (sugarcane bagasse and filter cake and vermiculite. Banana plantlets produced in pressed blocks and inoculated with AMF let to better results than their counterparts grown in tubes, increasing the height, shoot dry weight, contents of N, P and K in 90%, 829%, 2774%, 249% and 403%, respectively.

  5. In vitro Regeneration of Plantlets from Leaf and Nodal explants of Aristolochia indica L.- An Important Threatened Medicinal Plant

    Institute of Scientific and Technical Information of China (English)

    Pramod V. Pattar; M.Jayaraj

    2012-01-01

    Objective: An efficient reproducible protocol has been developed for in vitro regeneration of plantlets from leaf and nodal explants of Aristolochia indica L. Methods: Wild grown plants Aristolochia indica L. were collected and grown in the departmental garden. Leaf and nodal segments (0.5-1.0 cm) from young healthy plants were first washed thoroughly under running tap water for 15 - 20 minutes and then treated with liquid detergent [5% (v/v) Tween-20] for 5-10 minutes. Later these explants were washed with double-distilled water for 5 minutes. Subsequently, explants were immersed in 70% (v/v) ethanol for 2 - 3 minutes and washed with sterile glass double distilled water for 2-3 times. Eventually, the explants were treated with an aqueous solution of 0.1% (w/v) HgCl2 for 1 - 2 minutes and rinsed for two-to-three times in sterile ddH2O to remove all traces of HgCl2. The sterilized explants were inoculated aseptically onto solid basal Murashige and Skoog’s medium with different concentrations and combinations of BAP and NAA for in vitro regeneration of plants. Results: Both leaf and nodal explants cultured on MS medium supplemented with 0.8 mg/L BAP developed into mass of callus. These calli were subcultured for the induction of shoots and roots. Shoots were induced from both calli on MS medium supplemented with 0.8 mg/L BAP+0.5 mg/L NAA. Roots were induced from in vitro shoots on MS medium supplemented with 0.8 mg/L NAA for 4 weeks. Nodal explants were more regenerative with 95 % response compared to leaf explants with 85%. Finally, these in vitro regenerated plantlets were hardened, acclimatised and successfully transferred to the field. Conclusions: The present protocol for in vitro regeneration of Aristolochia indica L. can be used to make this plant available throughout the year for traditional healers, pharmaceutical usages, germplasm conservation, commercial cultivation, and also for the production of secondary metabolites.

  6. Behavior of Ilex paraguariensis St. Hilaire, 1822 to Meloidogyne incognita and M. paranaensis and their influence on development of plantlets

    Directory of Open Access Journals (Sweden)

    Débora Cristina Santiago

    2000-01-01

    Full Text Available The influence of Meloidogyne incognita and M. paranaensis on the development of mate (Ilex paraguariensis ST. Hill plantlets was assessed in greenhouse and laboratory. The experimental design was randomized. Every plot of each one of the 10 repetitions contained one mate plantlet , inoculated with 5,000 eggs of M. incognita (races 1, 2, 3 and 4 and M. paranaensis. After 120 days it was observed that mate was a reliable hostess to such nematodes and that they could finish their life cycle. Plant development was significantly modified by M. incognita, races 1 and 2. Dry and green matter weight of shoots changed according to treatment. No changes were observed in fresh root weight. The number of eggs per root was similar for M. incognita, races 1 and 2, and higher for the other treatments. The reproduction factor was above 1 for all treatments, indicating mate plants susceptibility to them.A influência de Meloidogyne incognita e M paranaensis no desenvolvimento de mudas de erva-mate, foi avaliada em casa-de-vegetação e laboratório. O delineamento experimental foi inteiramente casualizado, com 10 repetições, sendo cada parcela formada de 1 plântula de erva-mate/vaso, inoculada com 5000 ovos de M. incognita raças 1, 2, 3, 4 e M. paranaensis. Aos 120 dias, observou-se que a erva-mate, além de boa hospedeira destes nematóides, permitiu-lhes completar seu ciclo vital. O desenvolvimento das plantas foi significativamente afetado pelas raças 1 e 2 de M. incognita.; os pesos de matéria seca e fresca da parte aérea sofreram alterações conforme o tratamento. Não houve alteração no peso fresco de raízes e o número de ovos por raízes foi semelhante para as raças 1 e 2 de M. incognita, sendo, porém, mais elevado nos demais tratamentos. O fator de reprodução foi superior a 1 em todos os tratamentos, indicando a susceptibilidade da erva-mate a estes agentes.

  7. Graafikatriennaali grand prix Korea kunstnikule

    Index Scriptorium Estoniae

    1998-01-01

    Tallinna XI graafikatriennaali rahvusvaheline žürii andis grand prix korea kunstnikule Chung¡Sang-Gonile, kolm võrdset preemiat - soome kunstnikele Anita Jensenile ja Tapani Mikkonenile ning jaapani kunstnikule Estuko Obatale. Eesti Kunstimuuseumi preemia - Wendy Swallow. Tallinna linna preemia ja Ivar Luki sponsoripreemia - Walter Jule. Sponsoripreemiad : Paletti Eesti AS preemia - Inga Heamägi; Rannila Profiili preemia - Mojca Zlokarnik; UNDP preemia - Andrea Juan. Rotermanni soolalao arhitektuuri- ja kunstikeskuse diplom - Lis Ingram, Heli Päivikki Kurunsaari, Randi Strand, Wendy Swallow

  8. Grand unified theories from superstrings

    CERN Document Server

    Cleaver, G B

    1996-01-01

    I discuss how traditional grand unified theories, which require adjoint (or higher representation) Higgs fields for breaking to the standard model, can be contained within string theory. The status of stringy free fermionic three generation SO(10) SUSY--GUT models is reviewed. Progress in classification of both SO(10)_2 charged and uncharged embeddings and in N=1 spacetime solutions is discussed. Based on talks presented at the Workshop on SUSY Phenomena and SUSY GUTs, Santa Barbara, California, Dec. 7-11, 1995, and at the Orbis Scientiae, Coral Gables, Florida, January 25-28, 1996. To appear in the Proceedings of Orbis Scientiae, 1996.

  9. Grand canonical Molecular Dynamics Simulations

    CERN Document Server

    Fritsch, S; Junghans, C; Ciccotti, G; Site, L Delle; Kremer, K

    2011-01-01

    For simulation studies of (macro-) molecular liquids it would be of significant interest to be able to adjust/increase the level of resolution within one region of space, while allowing for the free exchange of molecules between (open) regions of different resolution/representation. In the present work we generalize the adaptive resolution idea in terms of a generalized Grand Canonical approach. This provides a robust framework for truly open Molecular Dynamics systems. We apply the method to liquid water at ambient conditions.

  10. Graafikatriennaali grand prix Korea kunstnikule

    Index Scriptorium Estoniae

    1998-01-01

    Tallinna XI graafikatriennaali rahvusvaheline žürii andis grand prix korea kunstnikule Chung¡Sang-Gonile, kolm võrdset preemiat - soome kunstnikele Anita Jensenile ja Tapani Mikkonenile ning jaapani kunstnikule Estuko Obatale. Eesti Kunstimuuseumi preemia - Wendy Swallow. Tallinna linna preemia ja Ivar Luki sponsoripreemia - Walter Jule. Sponsoripreemiad : Paletti Eesti AS preemia - Inga Heamägi; Rannila Profiili preemia - Mojca Zlokarnik; UNDP preemia - Andrea Juan. Rotermanni soolalao arhitektuuri- ja kunstikeskuse diplom - Lis Ingram, Heli Päivikki Kurunsaari, Randi Strand, Wendy Swallow

  11. Grand Gauge-Higgs Unification

    CERN Document Server

    Kojima, Kentaro; Yamashita, Toshifumi

    2011-01-01

    We propose a novel way to break grand unified gauge symmetries via the Hosotani mechanism in models that can accommodate chiral fermions. Adjoint scalar fields are realized through the so-called diagonal embedding method which is often used in the heterotic string theory. We calculate the one-loop effective potential of the adjoint scalar field in a five dimensional model compactified on an S^1/Z_2 orbifold, as an illustration. It turns out that the potential is basically the same as the one in an S^1 model, and thus the results in literatures, in addition to the chiral fermions, can be realized easily.

  12. Grand unification: quo vadis domine

    Energy Technology Data Exchange (ETDEWEB)

    Senjanovic, G.

    1985-01-01

    The present theoretical and experimental situation with grand unification is summarized. The issues of proton decay and the Weinberg angle are addressed, going through the predictions of both the standard SU(5) theory and its supersymmetric extension. The SO(10) theory, which provides a minimal one family model, is then studied. The gravitational characteristics of domain walls and strings are then discussed. It is argued that there is a need to go beyond SO(10) in order to incorporate a unified picture of families. This leads to the prediction of mirror fermions, whose physics is analyzed. 31 refs. (LEW)

  13. Grand Challenges of Enterprise Integration

    Energy Technology Data Exchange (ETDEWEB)

    Brosey, W.D; Neal, R.E.; Marks, D.

    2001-04-01

    Enterprise Integration connects and combines people, processes, systems, and technologies to ensure that the right people and the right processes have the right information and the right resources at the right time. A consensus roadmap for Technologies for Enterprise Integration was created as part of an industry/government/academia partnership in the Integrated Manufacturing Technology Initiative (IMTI). Two of the grand challenges identified by the roadmapping effort will be addressed here--Customer Responsive Enterprises and Totally Connected Enterprises. Each of these challenges is briefly discussed as to the current state of industry and the future vision as developed in the roadmap.

  14. Effect of cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry (Vaccinium corymbosum L.) plantlets grown in vitro.

    Science.gov (United States)

    Manquián-Cerda, K; Escudey, M; Zúñiga, G; Arancibia-Miranda, N; Molina, M; Cruces, E

    2016-11-01

    Cadmium (Cd(2+)) can affect plant growth due to its mobility and toxicity. We evaluated the effects of Cd(2+) on the production of phenolic compounds and antioxidant response of Vaccinium corymbosum L. Plantlets were exposed to Cd(2+) at 50 and 100µM for 7, 14 and 21 days. Accumulation of malondialdehyde (MDA), hydrogen peroxide (H2O2) and the antioxidant enzyme SOD was determined. The profile of phenolic compounds was evaluated using LC-MS. The antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power test (FRAP). Cd(2+) increased the content of MDA, with the highest increase at 14 days. The presence of Cd(2+) resulted in changes in phenolic compounds. The main phenolic compound found in blueberry plantlets was chlorogenic acid, whose abundance increased with the addition of Cd(2+) to the medium. The changes in the composition of phenolic compounds showed a positive correlation with the antioxidant activity measured using FRAP. Our results suggest that blueberry plantlets produced phenolic compounds with reducing capacity as a selective mechanism triggered by the highest activity of Cd(2+).

  15. American Grand Strategy: The End of Primacy?

    Science.gov (United States)

    2015-02-13

    harbingers of the end of American power or influence, we should note the new context of our international relationships and reassess our grand strategy as...AIR WAR COLLEGE AIR UNIVERSITY AMERICAN GRAND STRATEGY THE END OF PRIMACY? by Carey J. Jones, USAF, Lt Col A Research Report Submitted...Grand Strategy: The End of Primacy 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER

  16. EFFECT OF COPPER SULPHATE AND COBALT CHLORIDE ON GROWTH OF THE IN VITRO CULTURE TISSUES FOR DATE PALM (PHOENIX DACTYLIFERA L. CV. ASHGAR

    Directory of Open Access Journals (Sweden)

    Ahmed Madi Waheed AL-Mayahi

    2014-01-01

    Full Text Available This study was carried out to investigate the effects of copper sulphate and cobalt chloride on propagation of date palm cv. Ashgar in vitro. The rate of callus proliferation was significantly higher in the medium supplemented with 2 µM copper sulphate and 2 µM cobalt chloride together (p<0.05. Addition of copper sulphate and Cobalt chloride to the medium was most effective for shoots regeneration from callus and enhanced regeneration frequency as well as number of shoots obtained per explant, the best result (7.12 shoot/explant was obtained by using copper sulphate and Cobalt chloride at 2.0 µM. Also the results of this experiment indicates that the maximum induction of roots can be achieved in the media containing both additives at 0.5 µM. The callus exhibited a decline in carbohydrate contents, proteins, whereas total phenol content increased at high concentration of Cu and Co. The greatest formation of chlorophylls a, b and total chlorophyll was obtained in plantlets grown in the media containing both additives at 0.5 µM. As well as Maximum survivability was noticed for plants cultured in media that containing both copper sulphate and Cobalt chloride at 0.5 µM.

  17. Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain).

    Science.gov (United States)

    Chaurasia, Akhilesh Kumar; Patil, Hemant Bhagwan; Azeez, Abdul; Subramaniam, Vadakanthara Ramakrishnan; Krishna, Bal; Sane, Aniruddha Prafullachandra; Sane, Prafullachandra Vishnu

    2016-01-01

    The CONSTANS (CO) family is an important regulator of flowering in photoperiod sensitive plants. But information regarding their role in day neutral plants is limited. We report identification of nine Group I type CONSTANS-like (COL) genes of banana and their characterization for their age dependent, diurnal and tissue-specific expression. Our studies show that the Group I genes are conserved in structure to members in other plants. Expression of these genes shows a distinct circadian regulation with a peak during light period. Developmental stage specific expression reveals high level transcript accumulation of two genes, MaCOL3a and MaCOL3b, well before flowering and until the initiation of flowering. A decrease in their transcript levels after initiation of flowering is followed by an increase in transcription of other members that coincides with the continued development of the inflorescence and fruiting. CO binding cis-elements are observed in at least three FT -like genes in banana suggesting possible CO-FT interactions that might regulate flowering. Distinct tissue specific expression patterns are observed for different family members in mature leaves, apical inflorescence, bracts, fruit skin and fruit pulp suggesting possible roles other than flowering. This is the first exhaustive study of the COL genes belonging to Group I of banana.

  18. Influence of Plant Growth Regulators on In Vitro Shoot Multiplication and Plantlet Formation in Cassia angustifolia Vahl

    Directory of Open Access Journals (Sweden)

    Iram Siddique

    2015-10-01

    Full Text Available ABSTRACTAn effective and improved plant regeneration system was successfully developed using shoot tip explants taken from a two years old mature plant of Cassia angustifolia. The effect of different cytokinins, [6-benzyladenine (BA, Kinetin (Kin and thidiazuron (TDZ] at different concentrations (0.5-10 µM were evaluated as augmented with Murashige and Skoog (MS 1962 medium. Among all the cytokinins tested, TDZ (5.0 µM was optimum in inducing multiple shoots as compared to BA and Kin. The rate of shoot multiplication was increased when optimal concentration (5.0 µM of BA and Kin was tested with different concentration (0.1-1.0 µM of Indole-3- acetic acid (IAA. Among all the combinations tested, the maximum rate of shoot multiplication was obtained on MS medium supplemented with 5.0 µM BA and 0.5 µM IAA. The number of the shoots and shoot length developed in TDZ was increased when transferred to MS medium devoid of TDZ. After every subculture, rate of the shoot multiplication and shoot length showed increment and continued even after fifth subculture without any decline rate. In vitro rooting in regenerated shoots were best obtained in half-strength MS medium supplemented with 2.0 µM indole-3- butyric acid (IBA. Plantlets with well-developed shoot and roots were successfully hardened off in earthen pots containing garden soil and grown in greenhouse with 80% survival rate.

  19. Cryopreservation of chayote (Sechium edule JACQ. SW.) zygotic embryos and shoot-tips from in vitro plantlets.

    Science.gov (United States)

    Abdelnour-Esquivel, Ana; Engelmann, Florent

    2002-01-01

    This paper presents the development of cryopreservation protocols for zygotic embryos and apices of chayote (Sechium edule Jacq. Sw.), a tropical plant species with recalcitrant seeds. Zygotic embryos of two cultivars, Ccocro negro (CN) and Claudio (Cl) could withstand cryopreservation, with survival percentages of 10 and 30 %, after desiccation to 23 and 19 % moisture content (fresh weight basis), respectively. Apices sampled on in vitro plantlets of cultivars Cl, 13 and JM were successfully cryopreserved using a vitrification technique. Optimal conditions included the culture of mother-plants for 22 days on medium containing 0.3 M sucrose, culture of excised apices on the same medium for 1 day, loading of apices for 20 min with 2M glycerol + 0.4M glycerol, treatment with a series of diluted PVS2 solution (60 % PVS2 followed by 80 % PVS2 solution for 15 min (cultivar Cocoro Blanco [CB]) or 30 min (cultivars CN and Cl) at each concentration), rapid freezing and thawing, washing of shoot-tips with a 1.2 M sucrose solution, followed by recovery on media with progressively decreasing sucrose concentrations until the standard concentration of 0.1 M was reached. The highest survival percentages achieved ranged between 17 and 38 %, depending on the cultivar.

  20. Structural interaction between GFP-labeled diazotrophic endophytic bacterium Herbaspirillum seropedicae RAM10 and pineapple plantlets 'Vitória'

    Directory of Open Access Journals (Sweden)

    Lílian Estrela Borges Baldotto

    2011-03-01

    Full Text Available The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.

  1. 77 FR 11575 - Notice of Inventory Completion: Grand Rapids Public Museum, Grand Rapids, MI

    Science.gov (United States)

    2012-02-27

    ... National Park Service Notice of Inventory Completion: Grand Rapids Public Museum, Grand Rapids, MI AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Grand Rapids Public Museum has completed an... Rapids Public Museum. Repatriation of the human remains and associated funerary objects to the...

  2. Desenvolvimento de mudas de bromélia (Neoregelia cruenta (R. Graham L. B. Smith cultivadas em diferentes substartos e adubação foliar Development of plantlets of bromeliad (Neoregelia cruenta cultivated in different substrates and leaf fertilizers

    Directory of Open Access Journals (Sweden)

    Clarissa Alves Ferreira

    2007-06-01

    Full Text Available As bromélias apresentam formas exóticas com uma grande diversidade de cores e de flores, constituindo importantes espécies para uso em paisagismo e floricultura. Em conseqüência disto, são muito comercializadas. Porém, parte das plantas que se encontram no mercado, ainda são provenientes do extrativismo. Esta situação é também reflexo do pequeno número de informações sobre técnicas de propagação e cultivo. Uma das limitações é o desconhecimento do tipo de substrato e adubação adequados ao cultivo destas espécies. Assim, o objetivo deste trabalho foi avaliar o crescimento de bromélias do Neoregelia cruenta, cultivadas em diferentes substratos e adubações foliares através da altura, do número de folhas, da matéria fresca e seca, da parte aérea e das raízes. As mudas utilizadas foram provenientes de cultura de tecidos. Após um período de pré-aclimatização, foram transplantadas para estufa sem nebulização. Os substratos foram constituídos da mistura de diferentes proporções de solo, areia, casca de arroz carbonizada e por um substrato comercial à base de vermiculita. Aos substratos foi aplicada adubação foliar, combinando uréia e sacarose, em intervalos de quinze dias. Os resultados obtidos mostraram que as interações entre os substratos, dose de sacarose e de uréia, não afetaram a altura das mudas e o número de folhas. O uso de sacarose também não influenciou o desenvolvimento das mudas. O substrato comercial à base de vermiculita, independente da aplicação de adubação foliar, proporcionou maior altura das mudas e número de folhas. A aplicação de uréia apresentou efeito linear crescente durante o período avaliado.The bromeliads present exotic forms with a great variety of colors and flowers and are considered as important species used in landscape design and floriculture. As a consequence, these species are well commercialized. However, a part of these plants still come from natural

  3. Acclimatization and leaf anatomy of micropropagated fig plantlets Aclimatização e anatomia foliar de plântulas de figo micropropagadas

    Directory of Open Access Journals (Sweden)

    Chrystiane Fráguas Chirinéa

    2012-12-01

    Full Text Available The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.A sobrevivência de plantas micropropagadas durante e após a aclimatização é um processo limitante para o estabelecimento de plantas. Poucas informações são encontradas de como a anatomia de órgãos vegetativos de Ficus carica pode ser afetada pelas condições de cultivo e aclimatização. O presente trabalho teve por objetivo estudar os efeitos do tempo em meio de cultura e substratos durante a aclimatização de plântulas de figueira produzidas in vitro e cultivadas no campo. Plântulas multiplicadas in vitro foram separadas e transferidas para meio WPM, onde foram mantidas por 0; 15; 30; 45 e 60 dias. Diferentes

  4. Grand challenges for biological engineering.

    Science.gov (United States)

    Yoon, Jeong-Yeol; Riley, Mark R

    2009-09-22

    Biological engineering will play a significant role in solving many of the world's problems in medicine, agriculture, and the environment. Recently the U.S. National Academy of Engineering (NAE) released a document "Grand Challenges in Engineering," covering broad realms of human concern from sustainability, health, vulnerability and the joy of living. Biological engineers, having tools and techniques at the interface between living and non-living entities, will play a prominent role in forging a better future. The 2010 Institute of Biological Engineering (IBE) conference in Cambridge, MA, USA will address, in part, the roles of biological engineering in solving the challenges presented by the NAE. This letter presents a brief outline of how biological engineers are working to solve these large scale and integrated problems of our society.

  5. Grand challenges for biological engineering

    Directory of Open Access Journals (Sweden)

    Riley Mark R

    2009-09-01

    Full Text Available Abstract Biological engineering will play a significant role in solving many of the world's problems in medicine, agriculture, and the environment. Recently the U.S. National Academy of Engineering (NAE released a document "Grand Challenges in Engineering," covering broad realms of human concern from sustainability, health, vulnerability and the joy of living. Biological engineers, having tools and techniques at the interface between living and non-living entities, will play a prominent role in forging a better future. The 2010 Institute of Biological Engineering (IBE conference in Cambridge, MA, USA will address, in part, the roles of biological engineering in solving the challenges presented by the NAE. This letter presents a brief outline of how biological engineers are working to solve these large scale and integrated problems of our society.

  6. Grand Unification in Neutron Stars

    CERN Document Server

    Kaspi, Victoria M

    2010-01-01

    The last decade has shown us that the observational properties of neutron stars are remarkably diverse. From magnetars to rotating radio transients, from radio pulsars to `isolated neutron stars,' from central compact objects to millisecond pulsars, observational manifestations of neutron stars are surprisingly varied, with most properties totally unpredicted. The challenge is to establish an overarching physical theory of neutron stars and their birth properties that can explain this great diversity. Here I survey the disparate neutron stars classes, describe their properties, and highlight results made possible by the Chandra X-ray Observatory, in celebration of its tenth anniversary. Finally, I describe the current status of efforts at physical `grand unification' of this wealth of observational phenomena, and comment on possibilities for Chandra's next decade in this field.

  7. Grandes almacenes en Ginebra, Suiza

    Directory of Open Access Journals (Sweden)

    Braillard, P.

    1968-10-01

    Full Text Available This large building, whose metal structure is one of the most important ones erected in Switzerland, has 14 storeys. The sales zones occupy all the above ground floor levels, and the basements contain garages and stores. Additionally, there is a restaurant on the third floor, and administrative offices in the fourth floor of this outstanding commercial building, the «La Placette» Stores, of Geneva.Este gran edificio, cuya estructura metálica es una de las mayores construidas en Suiza, fue organizado en 14 niveles. Las plantas baja y superiores están destinadas a «venta»; y los sótanos, a garajes, almacenes, etc. La tercera planta alberga, además, el comedor, restaurante, etc.; y la cuarta, los locales de administración y dirección de este notable edificio comercial: «Grandes Almacenes La Placette», de Ginebra.

  8. Grand unification of neutron stars.

    Science.gov (United States)

    Kaspi, Victoria M

    2010-04-20

    The last decade has shown us that the observational properties of neutron stars are remarkably diverse. From magnetars to rotating radio transients, from radio pulsars to isolated neutron stars, from central compact objects to millisecond pulsars, observational manifestations of neutron stars are surprisingly varied, with most properties totally unpredicted. The challenge is to establish an overarching physical theory of neutron stars and their birth properties that can explain this great diversity. Here I survey the disparate neutron stars classes, describe their properties, and highlight results made possible by the Chandra X-Ray Observatory, in celebration of its 10th anniversary. Finally, I describe the current status of efforts at physical "grand unification" of this wealth of observational phenomena, and comment on possibilities for Chandra's next decade in this field.

  9. Proton decay and grand unification

    CERN Document Server

    Senjanovic, Goran

    2009-01-01

    I review the theoretical and experimental status of proton decay theory and experiment. Regarding theory, I focus mostly, but not only, on grand unification. I discuss only the minimal, well established SU(5) and SO(10) models, both ordinary and supersymmetric. I show how the minimal realistic extensions of the original Georgi - Glashow model can lead to interesting LHC physics, and I demonstrate that the minimal supersymmetric SU(5) theory is in perfect accord with experiment. Since no universally accepted model has of yet emerged, I discuss the effective operator analysis of proton decay and some related predictions from a high scale underlying theory. A strong case is made for the improvement of experimental limits, or better the search of, two body neutron decay modes into charged kaons and charged leptons. Their discovery would necessarily imply a low energy physics since they practically vanish in any theory with a desert in energies between M_W and M_GUT.

  10. Grand unification of neutron stars

    Science.gov (United States)

    Kaspi, Victoria M.

    2010-01-01

    The last decade has shown us that the observational properties of neutron stars are remarkably diverse. From magnetars to rotating radio transients, from radio pulsars to isolated neutron stars, from central compact objects to millisecond pulsars, observational manifestations of neutron stars are surprisingly varied, with most properties totally unpredicted. The challenge is to establish an overarching physical theory of neutron stars and their birth properties that can explain this great diversity. Here I survey the disparate neutron stars classes, describe their properties, and highlight results made possible by the Chandra X-Ray Observatory, in celebration of its 10th anniversary. Finally, I describe the current status of efforts at physical “grand unification” of this wealth of observational phenomena, and comment on possibilities for Chandra’s next decade in this field. PMID:20404205

  11. Instant OpenCV for iOS

    CERN Document Server

    Kornyakov, Kirill

    2013-01-01

    Filled with practical, step-by-step instructions and clear explanations for the most important and useful tasks. This book uses a very practical approach, with each recipe and their associated sample projects or examples focusing on a particular aspect of the technology.This book is intended for OpenCV developers who are interested in porting their applications to the iOS platform. Basic experience with OpenCV, computer vision, Objective C, and other iOS tools is encouraged.

  12. 农杆菌介导的葡萄柚转化再生体系建立研究%Establishment regeneration system for Citrus.paradisi Macf.cv Duncan by Agrobacterium-mediated transformation

    Institute of Scientific and Technical Information of China (English)

    韩美丽; 陆荣生; 林睿; 马跃峰; 杨玉霞; 覃建林

    2013-01-01

    [Objective]Transformation regeneration system of epicotyls derived from Citrus paradisi Macf.cv Duncan seedling were established in order to provide useful reference for its gene transformation.[Method]Epicotyls of seedling were used as explants to investigate the influence factors on induction and differentiation of adventitious buds,rooting of plantlets,and genetic transformation.[Result]The induction rate of adventitious buds derived from epicotyles were ranged from 72.7% to 81.6% in media with 0.50-1.00 mg/L TDZ.Comparing three types of growth regulators,the higher rooting rate (81.3%) were observed in 1/2MT medium with 1.0 mg/L of ABT 6 rooting powder.Among different antibiotics,timentin showed better sterilizing effects than cefotaxime sodium and ceftriaxone sodium on agrobacterium-mediated transformation plantlets culture.Timentin had no inhibition on buds induction of explants.During initial transformation stage of buds,5-7 days of darkness culture was beneficial to induce Kan anti-buds.Among 7 transgenic plantlets,6 plantlets showed positive in PCR detection.[Conclusion] Regeneration system of Citrus paradisi Macf.cv Duncan ecopityls was established and provided base on its genetic transformation.%[目的]建立邓肯葡萄柚上胚轴遗传转化再生体系,为葡萄柚基因转化研究奠定基础.[方法]以邓肯葡萄柚上胚轴为外植体,研究上胚轴不定芽和生根诱导、遗传转化的影响因子.[结果]在添加TDZ 0.50~1.00 mg/L的培养基上,上胚轴不定芽诱导率为72.7%~81.6%;无菌枝条的最适生根条件为1/2MT添加ABT6号1.0 mg/L,生根率可达81.3%.不同抗菌素中,以替门汀杀菌效果较好,且对外植体出芽无明显抑制作用.转化芽选择初期,5~7 d的暗培养有利于卡那霉素抗性芽的诱导发生.PCR检测结果显示,7株抗性植株中有6株呈阳性.[结论]建立了邓肯葡萄柚上胚轴植株再生繁殖体系,为其基因转化奠定了基础.

  13. 奉化大芋艿脱毒苗的组培快繁研究%Study on Virus-free Plantlets Rapid Propagation of Colocasia esculenta cv. Fenghuayunaitou

    Institute of Scientific and Technical Information of China (English)

    詹忠根; 徐程

    2006-01-01

    组织培养试验表明,培养在MS+6-BA 2 mg/L+NAA 0.2 mg/L上的奉化大芋艿茎尖,不定芽诱导率可达75%,月增殖系数为4倍.若在上述培养基中添加芋头汁、椰子汁等有机物则可实现不定芽继代繁殖和诱导生根一步完成,0.5~1 mg/L的三十烷醇在壮苗过程中的应用进一步提高了芋苗的根数、根长、苗高和鲜重.

  14. Study on the accumulation of dry matter and NPK in Pineapple(Ananas comosus cv.Comte de Paris)plantlet%巴厘菠萝干物质和NPK养分累积规律研究

    Institute of Scientific and Technical Information of China (English)

    陈菁; 孙光明; 臧小平; 陆新华; 刘胜辉

    2010-01-01

    在8月中旬种植大小基本一致的巴厘菠萝小苗,然后在其生长发育的11个时间段测定各器官生物量和NPK养分含量.结果表明:植株干物质和NPK养分累积可分为4个阶段.第一阶段为缓慢累积阶段;第二阶段为快速累积阶段,此阶段干物质和NPK养分累积量分别占收获时累积量的39.6%、50.8%、45.8%、54.6%:第三阶段为累积最快阶段,此阶段干物质和NPK养分累积量分别占收获时累积量的19.5%、20.2%、17.8%、12_3%;第四阶段为缓慢累积阶段.此阶段干物质、P素还有一个显著累积,但N、K累积量已很少.2、叶片、茎、根、果柄干物质和NPK养分累积量在见红期以前是随着菠萝果实生长而逐步增加,在果实发育中后期逐步下降,而果实和芽十物质和NPK养分累积量从果实发育至收获是逐步增加的.

  15. Google Earth Grand Tour Themes

    Science.gov (United States)

    De Paor, D. G.; Whitmeyer, S. J.; Bentley, C.; Dordevic, M. M.

    2014-12-01

    As part of an NSF TUES Type 3 project entitled "Google Earth for Onsite and Distance Education (GEODE)," we are assembling a "Grand Tour" of locations on Earth and other terrestrial bodies that every geoscience student should know about and visit at least in virtual reality. Based on feedback from colleagues at previous meetings, we have identified nine Grand Tour themes: "Plates and Plumes," "Rocks and Regions," "Geology Through Time," "The Mapping Challenge*," "U.S. National Parks*," "The Magical Mystery Tour*," "Resources and Hazards," "Planets and Moons," and "Top of the Pops." Themes marked with an asterisk are most developed at this stage and will be demonstrated in real time. The Mapping Challenge invites students to trace geological contacts, measure bedding strike and dip and the plunge, trend, and facing of a fold. There is an advanced tool for modeling periclinal folds. The challenge is presented in a game-like format with an emphasis on puzzle-solving that will appeal to students regardless of gender. For the tour of U.S. national parks, we divided the most geologically important parks into four groups—Western Pacific, West Coast, Rockies, and East Coast. We are combining our own team's GigaPan imagery with imagery already available on the Internet. There is a great deal of imagery just waiting to be annotated for geological education purposes. The Magical Mystery Tour takes students to Google Streetview locations selected by instructors. Students are presented with questions or tasks and are given automatic feedback. Other themes are under development. Within each theme, we are crowd-sourcing contributions from colleagues and inviting colleagues to vote for or against proposed locations and student interactions. The GEODE team includes the authors and: Heather Almquist, Stephen Burgin, Cinzia Cervato, Gene Cooper, Paul Karabinos, Terry Pavlis, Jen Piatek, Bill Richards, Jeff Ryan, Ron Schott, Kristen St. John, and Barb Tewksbury.

  16. Proton hexality in local grand unification

    Energy Technology Data Exchange (ETDEWEB)

    Foerste, Stefan; Nilles, Hans Peter [Bonn Univ. (Germany). Bethe Center for Theoretical Physics and Physikalisches Institut; Ramos-Sanchez, Saul [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Vaudrevange, Patrick K.S. [Muenchen Univ. (Germany). Arnold Sommerfeld Center for Theoretical Physics

    2010-07-15

    Proton hexality is a discrete symmetry that avoids the problem of too fast proton decay in the supersymmetric extension of the standard model. Unfortunately it is inconsistent with conventional grand unification. We show that proton hexality can be incorporated in the scheme of ''Local Grand Unification'' discussed in the framework of model building in (heterotic) string theory. (orig.)

  17. The Promise and Pitfalls of Grand Strategy

    Science.gov (United States)

    2012-08-01

    clarify the analysis that follows.3 There is no single, universally accepted definition of grand strategy. The British military historian Sir Basil ...presidential campaign—that American policy had come loose from its democratic moorings . When it comes to grand strategy, working within the strictures

  18. 33 CFR 117.285 - Grand Canal.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Grand Canal. 117.285 Section 117.285 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.285 Grand Canal. (a) The draw of the Lansing Island bridge, mile 0.7, shall open on...

  19. Grand Unification, Higgs Bosons, and Baryogenesis

    Science.gov (United States)

    Sher, Marc

    2004-03-01

    My task in these lectures is to discuss "Grand Unification and Higgs Bosons". Given that each of these subjects has had books written about them, this is a daunting task. My goal will be to introduce the basics of each topic, and provide references for those who wish to explore the topics further. I'll begin with a general motivation for grand unification, followed with an elementary review of SU(N) group algebra. The seminal SU(5) model will be discussed, followed by the supersymmetric version. On the second day, we'll look at other grand unified theories, and then look at the various methods of supersymmetry breaking in the context of grand unification. The third day, we'll turn to the Higgs mechanism, the effective potential, and mass bounds in the Standard Model and the MSSM. Finally, we'll look at baryogenesis, first in grand unified theories and then in the electroweak model.

  20. Cobertura morta do solo no cultivo de alface cv. Regina 2000, em Ji-Paraná/RO Mulching in lettuce cv. Regina 2000, in Ji-Paraná/RO

    Directory of Open Access Journals (Sweden)

    Jimmy Elizio de Carvalho

    2005-10-01

    Full Text Available Conduziu-se este estudo com o objetivo de avaliar o efeito de diferentes materiais de cobertura do solo na produtividade da alface (Lactuca sativa L. cv. Regina 2000. O delineamento experimental utilizado foi o de blocos casualizados, utilizando-se cinco tratamentos (palha de arroz, palha de café, Brachiaria brizantha L., serragem, testemunha sem cobertura morta e quatro repetições. A produtividade foi avaliada pelo número de folhas por planta, acúmulo de massa fresca e massa seca. Verificou-se que todos os materiais empregados como cobertura, controlaram a infestação de plantas invasoras. Entretanto, verificou-se uma grande infestação na testemunha promovendo redução de produtividade.The purpose of this research was to evaluate the effect of different mulching materials on lettuce (Lactuca sativa L. cv. Regina 2000 yield. The experimental design was randomized blocks, with five treatments (rice straw, coffee husk, Brachiaria brizantha L., sawing and control without mulching and four repetitions. The number of leaves per plant and the dry and fresh matter accumulation were evaluated. All materials used as mulching, controlled the weed infestation. However, weed population was greater in the control whit reduction yield in comparison to the others treatments.

  1. Isolation, culture and plantlet regeneration from cotyledon and mesophyll protoplasts of two pickling cucumber (Cucumis sativus L.) genotypes.

    Science.gov (United States)

    Punja, Z K; Tang, F A; Sarmento, G G

    1990-07-01

    Optimal protoplast yields from cotyledons (2.0×10(6) protoplasts/ 0.5 g tissue) and from true leaves (5.0×10(6) protoplasts/g tissue) of two Cucumis sativus genotypes were obtained following a 16 h digestion with, respectively, 1.25% pectinase+0.5% Cellulysin and 0.5 % pectinase+ 1.0% Cellulysin. Enzyme solutions were prepared in modified MS medium containing half-strength major salts, full complement of minor salts and vitamins, 2% sucrose and 0.25 M mannitol. A plating density of 3.5-4.0× 10(4) protoplasts/ml or higher was required for sustained division, with first division occurring in 6-7 days, second-third division in 8-9 days, and minicalli formation by day 13. Embedding in 0.4% agarose provided the highest plating efficiency (proportion that formed minicalli) of mesophyll protoplasts, which was 28.3% for genotype 3672 and 15% for genotype 3676. By comparison, liquid culture and droplet culture gave lower plating efficiencies (10-19%). Cotyledon and mesophyll protoplasts of one genotype formed minicalli on MS medium containing 2,4-D/BA at 1.0/2.5 μM and 5.0/5.0 μM, respectively, within 21 days, while mesophyll protoplasts of the second genotype formed minicalli on MS medium containing NAA/BA at 5.0/5.0 μM within 12 days. Shoot buds or somatic embryos were obtained upon subculture of calli to MS medium containing lower concentrations (0.05-0.01 μM) of 2,4-D/BA or NAA/BA and a few plantlets, ca.18, were recovered on hormone-free medium.

  2. 雪莲果试管苗快繁技术研究%Rapid Propagation of Yacon Plantlets in vitro

    Institute of Scientific and Technical Information of China (English)

    吴亚京; 艾辛; 蔡雁平

    2014-01-01

    以雪莲果(Smallanthus Sonchifolius (Poepp.End1.)H.Robinson)带腋芽的茎段为试验材料,研究雪莲果试管苗快繁技术。结果表明:MS+2,4-D 0.15mg·L-1+蔗糖(30g·L-1)+琼脂(6g·L-1)对雪莲果的茎段成苗效果最佳,成苗率达到95%,20d 生根率100%,平均根长6.4cm,培养25d,平均苗高6.2cm;以腐质土:珍珠岩=3:1为基质,对组培苗进行移栽,成活率可达97%。%In this study,yacon stem s with axillary buds were used as experim entalm aterials to explore the rapid propagation of yacon in suitable conditions.The resultsshowthatM S + 2,4-d 0.15m g/L + sucrose (30g/L)+ agar(6g/L)wasofthe optim aleffecton plantletssprouted from the yacon stem s with the rooting rate at100% and average rootlength of 6.4cm after 20 d,and the seedling rate at95% and average heightof6.2 cm after25 d;and the garden soil∶coarse sand∶peat= 1∶1∶1 as a substrate was used fortissue -cultured plantlets to be transplanted on,the seedling survivalrate was97% .

  3. Impact Of Thermotherapy And Chlorothalonil On Plantlets Production Of Some Genotypes Of Cassava Manihot Esculenta Crantz Produce In Benin

    Directory of Open Access Journals (Sweden)

    J.A.

    2015-08-01

    Full Text Available Cassava Manihot esculenta is a starchy root plant of great economic importance in sub-Saharan Africa and particularly in Benin. Its production is confronted to virus diseases which cause a considerable losses of yield. This work aims to determine the impact of thermotherapy and chlorothalonil in the production of cassava material of plantation. Cuttings of four varieties RB89509 BEN86052 9102319 92B0057 are cultivated under two conditions of thermotherapy and a control under greenhouse during 4 weeks. These different conditions are a closed drying oven with 16 hours photoperiod at 40 C the day and 36C the night a drying oven Binder with photoperiod of 12 hours at 38C the day and 28C the night and the control carried out under the conditions of the greenhouse. The media used was Murashige and Skoog MS added with various amounts of chlorothalonil 0.6 gl and 2gl and control without chlorothalonil. Both techniques of thermotherapy eliminate the virus symptoms of cassava at the rate of 0 seedling infected in thermotherapy against 16 seedlings in natural condition. The technique of closed drying oven significantly favors the production of nodes at 5 level p0.000 and shoots p0.02 on the other hand Binder drying oven has no significant effect on the production of shoots p0.68. The chlorothalonil had a positive effect on in vitro infestations elimination of cassava p0.05 but influenced the growth and development of cassava explants by reducing of nodes production p0.01 without a lethal effect on the plantlets until the dose of 2gl.

  4. The early-type close binary CV Velorum revisited

    NARCIS (Netherlands)

    Yakut, K.; Aerts, C.; Morel, T.

    2007-01-01

    Aims.Our goal was to improve the fundamental parameters of the massive close double-lined eclipsing B2.5V+B2.5V binary CV Velorum. Methods: We gathered new high-resolution échelle spectroscopy on 13 almost consecutive nights covering two orbits. We computed a simultaneous solution to all the availab

  5. Anthelmintic Activity of Musa paradisiaca (L. cv. Puttabale

    Directory of Open Access Journals (Sweden)

    Venkatesh, V. Krishna

    2013-04-01

    Full Text Available Musa paradisiaca cv. Puttabale (AB group is an indigenous banana cultivar commonly cultivated in the Malnad region of Karnataka, India. Helminthes infections are acute and chronic illness in human beings and cattle. About 3 million people are infected with helminthes worldwide. Traditionally, the plant M. paradisiaca cv. Puttabale was used to expel parasitic worms. In order to justify the ethanomedicinal claim with scientific report, sincere attempts have been made to investigate the Anthelmintic activity from corm ethanol extracts of M. paradisiaca cv. Puttabale using Pheretima posthuma as an experimental model. Three concentrations of 25, 50 and 100 mg/ml of corm ethanol extract were used to study their effect in time of paralysis and death of worm. The results suggest that the ethanol extract at the concentration of 100 mg/ml showed significant effect in time of paralysis at 42.33±1.45 min and death time was 54.00±0.58 min than control group in time of paralysis (142.67±1.45 min and death (168.00±1.53 min. Standard drug piperazine citrate showed paralysis on 39.67±0.88 min and death at 59.00±0.58 min. The corm ethanol extract confirmed antihelmintic activity in dose depend manure and efficient, than standard drug piperazine citrate. This investigation revealed that the antihelmintic property of ethanol extracts of Musa paradisiaca cv. Puttabale against Pheretima posthuma to support its medicinal claims.

  6. Avaliação da cv. Tannat para elaboração de vinho tinto Evaluation of cv. Tannat to elaborate red wine

    Directory of Open Access Journals (Sweden)

    Luiz Antenor Rizzon

    2004-06-01

    Full Text Available A uva Tannat, originária do Sudoeste da França, foi introduzida no Rio Grande do Sul, pela Estação Experimental de Caxias do Sul. É uma cultivar difundida na Serra Gaúcha para elaboração de vinho tinto, utilizado para corte e para a produção de vinho varietal. O objetivo do presente trabalho foi avaliar o potencial enológico da cv. Tannat para a elaboração de vinho tinto fino nessa região vitícola. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho nas safras de 1987 a 1994, a partir de um vinhedo localizado em Bento Gonçalves, RS. Os resultados evidenciaram que a cv. Tannat tem cacho com peso médio de 216,1g e baga esférica pequena (1,54g. O mosto apresenta teor elevado de acidez titulável (155meq/L-1. O vinho se caracteriza por teores elevados em: polifenóis totais (I 280= 43,0, taninos (1,9g/L, antocianinas (539mg/L, potássio (1.856mg/L-1 e soma dos álcoois superiores (512mg/L-1. As características sensoriais do vinho Tannat na Serra Gaúcha evidenciam uma cor vermelha-violácea intensa, tânicos, nervosos, relativamente duros e aromas de frutas vermelhas. É recomendável para corte com outros vinhos tintos finos para intensificar sua cor e estrutura e também para a produção de vinho varietal podendo melhorar com o amadurecimento em barricas de carvalho.The Tannat grape is native to the Southwest of France, and was introduced into the State of Rio Grande do Sul by the Caxias do Sul Experimental Station. It is a cultivar diffused in the Serra Gaucha region with the purpose of elaborating varietal red wine for blending. The purpose of this work was to evaluate the enological potential of the Tannat grape for red wine in this region. To achieve this objective, variables related to the berries, clusters, must, and wine were evaluated from the 1987 to 1994 vintages, in a vineyard established in Bento Gonçalves, RS, Brazil. The results showed that Tannat grapes have large clusters (216.1g and

  7. Avaliação da cv. Merlot para elaboração de vinho tinto Evaluation of cv. Merlot to make red wine

    Directory of Open Access Journals (Sweden)

    Luiz Antenor Rizzon

    2003-12-01

    Full Text Available A uva Merlot é uma das responsáveis pelas características dos vinhos tintos de Saint Émillion, região de Bordeaux, França. Foi introduzida no Rio Grande do Sul através da Estação Agronômica de Porto Alegre. É, atualmente, uma das cultivares tinta de Vitis vinifera L. com maior volume de produção da Serra Gaúcha. É utilizada para a elaboração de vinho tinto para ser consumido jovem. O objetivo do presente trabalho foi avaliar o potencial enológico da cv. Merlot para a elaboração de vinho tinto fino. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho nas safras de 1987 a 1994, em Bento Gonçalves, RS. Os resultados evidenciaram que a cv. Merlot tem cacho com peso médio de 161,9g e baga esférica e pequena, pesando 1,61g. No mosto, o valor médio de açúcar é de 18,4ºBrix; o da acidez total, de 104meqL-1; e o do pH, de 3,21. O vinho se caracteriza por ter teores médios de 10,8% v/v de álcool; 70,3meqL-1 de acidez titulável; e pH de 3,49. Sob o aspecto sensorial, o vinho Merlot apresenta cor vermelho-violáceo com boa intensidade. Gustativamente distingue-se pela maciez, equilíbrio e fineza.Merlot is a traditional grape of the Saint Émillion wines, Bordeaux region - France. It was introduced into, the State of Rio Grande do Sul by the Estação Agronômica de Porto Alegre, Brazil. Today, of the red grape cultivars it shows the largest production in the Serra Gaúcha region. The purpose of this work was to evaluate the enological potential of Merlot grapes for red winemaking. To achieve this purpose, variables related to berry clusters, must and wine were measured between 1987 and 1994 vintages, from Merlot vineyards located in the municipality of Bento Gonçalves. The results showed that Merlot grapes have clusters of medium size (161.9g and small berries (1.61g. The must showed adequate sugar level (18.4ºBrix, high titratable acidity (104mqL-1 and medium pH 3.21. The wine Merlot had an

  8. La Grande: volver a empezar

    Directory of Open Access Journals (Sweden)

    Julio Premat

    2011-07-01

    Full Text Available Este texto, borrador de un trabajo más amplio, pretende despejar algunas pistas de lectura de La grande, en tanto que paradójico final de la producción de Saer. Digo “paradójico” porque puede tomársela como una novela de comienzo o de origen : de un volver a empezar, en todos los sentidos del término. En esa perspectiva podrían estudiarse algunos núcleos temáticos (como el retorno o el recuerdo de cara a la construcción del texto, a la relación planteada con la tradición y a la singular historia de su escritura (y al material genético que rodea y completa esta novela a la vez inacabada y póstuma. En esta intervención, la idea es la de comentar tres textos, escenas o frases del texto, y a partir de allí esbozar pistas para un estudio que está en ciernes.Première ébauche d’un travail de plus d’ampleur, ce texte vise à éclairer quelques pistes de lecture de La grande, en tant que fin paradoxale de la production de Saer. Je dis « paradoxale » parce que l’on peut considérer cette œuvre comme un roman des commencements ou des origines : comme un retour aux débuts, dans tous les sens du terme. Dans cette perspective, plusieurs nœuds thématiques (comme le retour ou le souvenir pourraient être étudiés en relation à la construction du texte, à la relation qui s’établit avec la tradition et à la singulière histoire de son écriture (et au matériau génétique qui entoure et complète ce roman, à la fois inachevé et posthume. Il sera question ici de commenter trois textes, scènes ou phrases du texte, et d’ébaucher à partir de là quelques pistes pour une étude à l’état naissant.The objective of this text, a draft for a broader work, is to outline some reading clues for La grande, inasmuch as it constitutes a paradoxical ending of Saer’s production. I say “paradoxical” because we can consider this work a novel of beginnings or of origins: a return to the beginning, in every meaning of the

  9. Responses of in vitro-grown plantlets (Vitis vinifera to Grapevine leafroll-associated virus-3 and PEG-induced drought stress

    Directory of Open Access Journals (Sweden)

    Zhenhua eCui

    2016-06-01

    Full Text Available AbstractStresses caused by viral diseases and drought have long threatened sustainable production of grapevine. These two stresses frequently occur simultaneously in many of grapevine growing regions of the world. We studied responses of in vitro-grown plantlets (Vitis vinifera to Grapevine leafroll associated virus-3 (GLRaV-3 and PEG-induced drought stress. Results showed that stress induced by either virus infection or drought had negative effects on vegetative growth, caused significant decreases and increases in total soluble protein and free proline, respectively, induced obvious cell membrane damage and cell death, and markedly increased accumulations of O2- and H2O2. Co-stress by virus and drought had much severer effects than single stress on the said parameters. Virus infection alone did not cause significant alternations in activities of POD, ROS and SOD, and contents of MDA, which, however, markedly increased in the plantlets when grown under single drought stress and co-stress by the virus and drought. Levels of ABA increased, while those of IAA decreased in the plantlets stressed by virus infection or drought. Simultaneous stresses by the virus and drought had co-effects on the levels of ABA and IAA. Up-regulation of expressions of ABA biosynthesis genes and down-regulation of expressions of IAA biosynthesis genes were responsible for the alternations of ABA and IAA levels induced by either the virus infection or drought stress and co-stress by them. Experimental strategies established in the present study using in vitro system facilitate investigations on ‘pure’ biotic and abiotic stress on plants. The results obtained here provide new insights into adverse effects of stress induced by virus and drought, in single and particularly their combination, on plants, and allow us to re-orientate agricultural managements towards sustainable development of the agriculture.

  10. Militari italiani e grande guerra

    Directory of Open Access Journals (Sweden)

    Emilio Franzina

    2015-01-01

    Full Text Available Il saggio verte su alcuni aspetti dei fenomeni immigratori che si collegano alla genesi e alla tenuta dei sensi di appartenenza etnica e nazionale. Accanto alla progressiva rimozione dei vecchi meccanismi di acculturazione linguistica, che scalzano, ove posseduto in partenza, l’italiano procurando la sua sostituzione progressiva –e in parte fisiologica– con il castigliano si assiste in America, per integrazione, anche al declino dell’“italianità politica”. Un momento di svolta prima del definitivo affermarsi di tale processo di “argentinizzazione” fu rappresentato però dalla congiuntura della grande guerra. Il suo deflagrare, infatti, coincise con il momento di massima espansione (non solo a Buenos Aires della presenza immigratoria straniera e di quella italiana in particolare. Diversamente da quanto succederà più tardi con il secondo conflitto mondiale, dopo quasi vent’anni però di declino o di arresto dei flussi in entrata, tra il 1914 e il 1918 si poté assistere infatti a una concreta forma di doppio patriottismo o di duplice lealtà politico-istituzionale da parte dei gruppi etnici immigratori all’interno dei quali si generarono scelte come la decisione presa da molti dei loro componenti di arruolarsi sotto le bandiere delle “antiche patrie” per raggiungere i campi di battaglia europei.

  11. An RNA-Seq Analysis of Grape Plantlets Grown in vitro Reveals Different Responses to Blue, Green, Red LED Light, and White Fluorescent Light.

    Science.gov (United States)

    Li, Chun-Xia; Xu, Zhi-Gang; Dong, Rui-Qi; Chang, Sheng-Xin; Wang, Lian-Zhen; Khalil-Ur-Rehman, Muhammad; Tao, Jian-Min

    2017-01-01

    Using an RNA sequencing (RNA-seq) approach, we analyzed the differentially expressed genes (DEGs) and physiological behaviors of "Manicure Finger" grape plantlets grown in vitro under white, blue, green, and red light. A total of 670, 1601, and 746 DEGs were identified in plants exposed to blue, green, and red light, respectively, compared to the control (white light). By comparing the gene expression patterns with the growth and physiological responses of the grape plantlets, we were able to link the responses of the plants to light of different spectral wavelengths and the expression of particular sets of genes. Exposure to red and green light primarily triggered responses associated with the shade-avoidance syndrome (SAS), such as enhanced elongation of stems, reduced investment in leaf growth, and decreased chlorophyll levels accompanied by the expression of genes encoding histone H3, auxin repressed protein, xyloglucan endotransglycosylase/hydrolase, the ELIP protein, and microtubule proteins. Furthermore, specific light treatments were associated with the expression of a large number of genes, including those involved in the glucan metabolic pathway and the starch and sucrose metabolic pathways; these genes were up/down-regulated in ways that may explain the increase in the starch, sucrose, and total sugar contents in the plants. Moreover, the enhanced root growth and up-regulation of the expression of defense genes accompanied with SAS after exposure to red and green light may be related to the addition of 30 g/L sucrose to the culture medium of plantlets grown in vitro. In contrast, blue light induced the up-regulation of genes related to microtubules, serine carboxypeptidase, chlorophyll synthesis, and sugar degradation and the down-regulation of auxin-repressed protein as well as a large number of resistance-related genes that may promote leaf growth, improve chlorophyll synthesis and chloroplast development, increase the ratio of chlorophyll a (chla

  12. High frequency in vitro plantlet regeneration and antioxidant activity ofEnicostema axillare (Lam.)Raynal ssp. littoralis (Blume)Raynal:An important medicinal plant

    Institute of Scientific and Technical Information of China (English)

    Kousalya Loganathan; VNarmatha Bai

    2014-01-01

    Objective:To develop a method for high frequency plantlets regeneration protocol forEnicostema axillare(Lam.)Raynal ssp.littoralis(Blume)Raynal(E. axillare) without intermediate callus phase and to determine the content and correlation of phenolic compounds and their antioxidant activity of both the plants derived from nature and nodal culture byDPPH assay.Methods:The nodal explants were cultured onMS basal medium fortified with different concentration of various growth regulators such asBAP,KIN,TDZ and2iP(0.5-2 mg/L) individually and in combinations with or withoutGA3 for shoot bud induction and multiplication.Total phenol and flavonoid content was determined in both plants from nature and nodal culture and antioxidant activity was determined byDPPH assay.Results:The highest number of multiple shoot(108.00±3.55 shoots/explants) was obtained onMS medium supplemented withBAP(2 mg/L) in combination withKIN (0.5 mg/L) andGA3(2 mg/L).Rooting was optimized on half-strengthMS medium supplemented withIAA(0.5 mg/L).The rooted plantlets were transferred to paper cups containing vermiculite and hardening was successfully attained with75% survival.Among the four extract of methanol and water extract from both the plantlet from nature and nodal culture, the concentration of flavonoid was found to be higher in methanol extract of the plants from nature(146.57±1.68 mg rutin/g extract) and phenol content was higher in the water extract of plant from nature(52.53±1.67 mgGAE /g extract).The radical scavenging activity of four extracts.Methanol extract of plant grown in nature showed the highest radical scavenging activity(IC50 =87.10 μg/mL) was investigated byDPPH test.Conculsion:The present study not only enables reinforcement of wild plant populations usingex situ growth of individuals, but it also helps for high scale production of plantlets.A high correlation between antioxidant capacities and their total phenolic contents indicated that flavonoid compounds were a major

  13. Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

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    Dikash Singh THINGBAIJAM

    2012-11-01

    Full Text Available Eleven cultivars of C. longa var. Lakadong were collected from Manipur having different topography. Curcumin content in different cultivars has been analyzed by using UV-Visible Spectrophotometer (100 Bio-Carry Spectrophotometer. The curcuminoids content were analyzed and quantified for identification of best quality cultivar. Thoubal Cultivar with highest curcumin content (9.44% was subjected for tissue culture technique using different culture vessels and silver nitrate for rapid multiplication and scaling up of microrhizome production. High multiplication rate of 27.40�0.47 were obtained in Murashige and Skoog�s medium supplemented with 3% sucrose + 1 mg L-1 ?-napthalene acetic acid, 4 mg L-1 6-benzyl-amino-purine and 11 ?M silver nitrate. Effect of different culture vessels and silver nitrate were studied for microrhizome and multiple shoots formation. Relatively higher rate of shoots along with microrhizome (17.5�0.32 can be seen in Growtek which was grown without any plant growth regulator. Growtek was used for scaling up of microrhizome production in vitro and utmost microrhizome was produced in liquid Murashige and Skoog�s medium supplemented with 8% sucrose, 1 mg L-1 ?-napthalene acetic acid, 4 mg L-1 6-benzyl-amino-purine and 11 ?M silver nitrate (36.25�0.27. Addition of silver nitrate in the medium resulted in improvement of microrhizome induction in vitro. Higher concentration of silver nitrate (33, 44, 66, 88 ?M negatively affected the microrhizome and shoot multiplication and shows inhibition of tissue response completely. Analysis of in vitro derived plantlets during acclimatization shows that the exogenous applied of silver nitrate shows superior growth as compared to control. 90-95% of plantlets with and 75-80% plantlets without silver nitrate treatment were successfully established under ex vitro acclimatization. The protocol could be utilized for large scale production of true-to-type plantlets and as alternative

  14. Rio Grande Channel, El Paso Area, 1852

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Historical map of Rio Grande river between Texas and Mexico. U.S.-Mexican Boundary Survey.The original map is a Xerox of a Photolithographic Copy of Salazar...

  15. Waterfowl Evaluation- Lake Ophelia / Grand Cote NWR's

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This memo describes the waterfowl evaluation on Lake Ophelia and Grand Cote National Wildlife Refuges. A team of multiagency biologist overviewed the waterfowl...

  16. Las cinco grandes dimensiones de la personalidad

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    Jan ter Laak

    1996-12-01

    Full Text Available Este artículo revisa las distintas posiciones teóricas sobre las cinco grandes dimensiones de la personalidad, mostrando las semejanzas y diferencias entre las posturas teóricas. Esta contribución presenta lo siguiente: (a la génesis del contenido y la estructura de las cinco dimensiones; (b la fortaleza de las cinco dimensiones; (e la relación de las cinco grandes dimensiones con otros constructos de personalidad; (d discute el valor predictivo de las puntuaciones del perfil de las cinco dimensiones para criterios pertinentes; (e analiza el estatus teórico de las cinco dimensiones; (f discute críticas históricas sobre las cinco grandes dimensiones y se formulan respuestas a estas críticas; (g hace conjeturas para el futuro de las cinco grandes dimensiones; y (h concluye con algunas conclusiones y comentarios.

  17. Rio Grande Channel, Guadalupe Area, 1852

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Historical map of Rio Grande river between Texas and Mexico. U.S.- Mexican Boundary Survey. The original map is a xerox of a map entitled Boundary between the United...

  18. A Grand Gathering of Storytelling Artists

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    The grand storytelling fair held in Majie Township, Hebei Province every year, has a history of more than 400 years. ● Most of the stories used to be based on ancient legends and folk stories. Nowadays, there are also many newly-written pieces about people’s lives in modern times. ● Every year, the grand storytelling performance attracts hundreds and thousands of locals and visitors from several neigh-boring provinces.

  19. Functional representation for the grand partition function of a multicomponent system of charged particles: Correlation functions of the reference system

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    O.V.Patsahan

    2006-01-01

    Full Text Available Based on the method of collective variables (CV with a reference system, the exact expression for the functional of the grand partition function of a m-component ionic model with charge and size asymmetry is found. Particular attention is paid to the n-th particle correlation functions of the reference system which is presented as a m-component system of "colour" hard spheres of the same diameter. A two-component model is considered in more detail. In this case the recurrence formulas for the correlation functions are found. A general case of a m-component inhomogeneous system of the "colour" hard spheres is also analysed.

  20. Performance Report and Improvements in CV Machine Areas

    CERN Document Server

    Monsted, A

    2000-01-01

    The performance of cooling and ventilation (CV) equipment is defined as its availability to the PS, SPS and LEP accelerators. Three teams for the accelerators plus a Utilities team, which receives performance information from the physics and technical control room, carry out the operation of this equipment. Collected performance statistics as well as data from the Computer Aided Maintenance Management system (CAMM) and supervision system provide information on the exact state of the CV equipment. This is used to more effectively schedule preventive maintenance which, by reducing system failures, results in improved equipment performance. This paper will present performance data and discuss the prospects for improving it through the use of an updated version of CAMM and a new supervision system.

  1. THE BIOTECHNOLOGY OF EMBRYOGENIC CELL LINES OBTAINING AND PLANTLETS OF CONIFEROUS SPECIES IN SIBERIA IN CULTURE IN VITRO

    Directory of Open Access Journals (Sweden)

    Tretiakova I.

    2012-08-01

    Full Text Available Experiments of culturing the immature isolated embryos and megagamethophytes of Siberian coniferous species were carried out on different modified media: ½ LV medium for Pinus sibirica and Pinus pumila, MSG and AI media (patent № 2431651 for Larix sibirica and Larix gmelinii, DCR medium for Picea ajanensis. For induction of embryogenic callus every species needs the optimized medium supplemented with L-glutamine, casein hydrolysate, ascorbic acid and hormones with different concentrations and their different proportions. The active proliferation of embryonal masses is observed on the same medium with reduced concentration of cytokinins. The proliferation of embryonal masses was significantly improved when they were subcultured after dispersing in liquid medium. The somatic embryos from embryonal masses are matured on basal medium with ABA (60-120 mM and PEG. In spite of species specificity the embryogenesis of morphogenic structures had the same scheme: elongation and asymmetric division of somatic cells, formation of initial cells and embryonal tubes, development of globular, torpedo and bipolar somatic embryos, embryos maturation and germination. However, not all donor-plants of coniferous species can form the embryogenic cell lines and somatic embryos. The active development of embryonal masses and formation of somatic embryos are observed from zygotic embryo of hybrid seeds of P. sibirica and L. sibirica. The obtained embryogenic lines were characterized by different proliferative activity. During 10 months cultivation the value of embryonal masses in different lines was 140-570 g. The number of somatic embryos varies from 210 to 410 per 100 mg of callus fresh weight. Decreasing proliferation activity did not observed during 24-45 months cultivation. However, development of somatic embryos in long cultivated lines decreased. Maturation of somatic embryos and development of plantlets were established in L. sibirica and P. pumila 50

  2. Spectroscopic Classification of ASASSN-16do as a CV

    Science.gov (United States)

    Strader, Jay; Chomiuk, Laura; Shishkovsky, Laura

    2016-04-01

    We obtained an optical spectrum of ASASSN-16do (ATel #8888) on UT April 17.07 with the Goodman Spectrograph on the SOAR telescope. The source has a blue continuum and broad double-peaked Balmer and He 5875 emission at z~0, with an H-alpha FWHM of about 2400 km/s. This value is high for a CV and suggests the source is observed close to edge-on.

  3. The draft genome of Corchorus olitorius cv. JRO-524 (Navin

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    Debabrata Sarkar

    2017-06-01

    Full Text Available Here, we present the draft genome (377.3 Mbp of Corchorus olitorious cv. JRO-524 (Navin, which is a leading dark jute variety developed from a cross between African (cv. Sudan Green and indigenous (cv. JRO-632 types. We predicted from the draft genome a total of 57,087 protein-coding genes with annotated functions. We identified a large number of 1765 disease resistance-like and defense response genes in the jute genome. The annotated genes showed the highest sequence similarities with that of Theobroma cacao followed by Gossypium raimondii. Seven chromosome-scale genetically anchored pseudomolecules were constructed with a total size of 8.53 Mbp and used for synteny analyses with the cocoa and cotton genomes. Like other plant species, gypsy and copia retrotransposons were the most abundant classes of repeat elements in jute. The raw data of our study are available in SRA database of NCBI with accession number SRX1506532. The genome sequence has been deposited at DDBJ/EMBL/GenBank under the accession LLWS00000000, and the version described in this paper will be the first version (LLWS01000000.

  4. Comparative studies about the influence of salicylic and acetylsalicilic acid on content of assimilatory pigments in the primary leaves of wheat (Triticum aestivum plantlets

    Directory of Open Access Journals (Sweden)

    Cornelia PURCAREA

    2007-05-01

    Full Text Available Salicylic acid (SA and some of its derivates are phenolic compounds recently recognized as plant growth regulators involved in many physiological processes including photosynthesis. One of the important derivates of Salicylic Acid is the Acetylsalicylic Acid. In the present investigation we studied the influence of exogenous Acetylsalicylic and Salicylic acid with different concentrations on the assimilatory pigments contents of the primary leaves of wheat seedlings in comparison with the same parameters of the control lots which were treated with water. The wheat seedlings were soaked for 6 hours in 0.01mM; 0.1mM; 0.5mM and 1 mM SA or ASA solutions and in water for the control lot, germinated for 7 days on filter paper moistened with water. After that, we planted the plantlets in sand and sprayed their coleoptiles and primary leaves, each day for an additional 7 days, with water. In the 14th days of germination we determined the content of assimilatory pigments extracted with N,N-dimethylformamide (DMF. The results showed that exogenous 0.01 mM, 0.1mM, 0.5 mM or 1.0 mM SA solution treatments cause more significant increases in the assimilatory pigments contents in leaves of wheat plantlets than treatments with ASA solutions of the same concentrations do.

  5. Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract.

    Science.gov (United States)

    Amini, Massoume; Deljou, Ali; Nabiabad, Haidar Saify

    2016-07-01

    Developmental deficiency of somatic embryos and regeneration to plantlets, especially in the case of transformation, are major problems of somatic embryo regeneration in alfalfa. One of the ways to overcome these problems is the use of natural plant regulators and nutrients in the culture medium of somatic embryos. For investigating the influence of Cuscuta campestris extract on the efficiency of plant regeneration and transformation, chimeric tissue type plasminogen activator was transferred to explants using Agrobacterium tumefaciens, and transgenic plants were recovered using medium supplemented with different concentration of the extract. Transgenic plants were analyzed by PCR and RT-PCR. Somatic embryos of Medicago sativa L. developed into plantlets at high frequency level (52 %) in the maturation medium supplemented with 50 mg 1(-1)C. campestris extract as compared to the medium without extract (26 %). Transformation efficiency was 29.3 and 15.2 % for medium supplemented with dodder extract and without the extract, respectively. HPLC and GC/MS analysis of the extract indicated high level of ABA and some compounds such as Phytol, which can affect the somatic embryo maturation. The antibacterial assay showed that the extract was effective against some strains of A. tumefaciens. These results have provided a scientific basis for using of C. campestris extract as a good natural source of antimicrobial agents and plant growth regulator as well, that can be used in tissue culture of transgenic plants.

  6. VIGOR OF PLANTLET FROM MICROPLANTLET TREATED BY FILTRATE AND CELL SUSPENSION OF SOME ISOLATES OF BACILLUS AND RESISTANCE TO BANANA WILT PATHOGEN AFTER ACCLIMATIZATION

    Directory of Open Access Journals (Sweden)

    Hadi wiyono

    2013-08-01

    Full Text Available Blood Disease Bacterium (BDB and Fusarium oxysporum f.sp. cubense (FOC is a couple wilt pathogen  of  banana.  These pathogens are the most important constraint in cultivation of banana in Indonesia.  In the integrated control strategy of the disease, the use of healthy seedlings produced from tissue culture technique is recommended.  The seedling produced by tissue culture technique however leads to lower vigor and susceptibility to the disease due to the aseptic work in vitro causing the beneficial bacterial endophytic to be eliminated. Therefore, the utility of the beneficial endophytic bacteria should be studied for recovering the vigor and resistance of the seedling.     Three isolates of endophytic Bacillus (B04, B05, B10 have been effective as growth promoter of microplantlet and antagonist of BDB and FOC in vitro.   Here then, this article reports the study results of the vigor of the plantlet (treated microplantlet by filtrate or cell suspension of the Bacillus after 3 months in acclimatization. The results were similar to the previous results on microplantlet in vitro, that Bacillus isolates B04, B05, and B10 were capable of promoting the growth and inducing the resistance to wilt pathogens on banana plantlets.  The treatments with bacterial cell inoculums were more effective than those bacterial filtrate. Isolate B10 was most potential followed by B05 and B04 respectively.

  7. In vitro growth and organogenesis of Prosopis farcta plantlets (Fabaceae, Mimosoideae) in culture medium supplemented with various concentrations of Ca++ and Na+.

    Science.gov (United States)

    Stambouli, S; Bouzid, S; Dutuit, P; Harzallah-Skhiri, Fethia

    2012-03-01

    The objective of this study was to vary the mineral composition of the culture medium of Prosopis farcta seedlings per addition of Na+ and Ca++ ions with the aim to identify the culture media which support the growth and/or the expression of the in vitro plant organogenesis. The Na+ and Ca++ ions were added in the culture medium in various concentrations by taking the Gamborg medium, in which macroelements were diluted 10 times, as the basic one. After two months of culture, parameters relating to the vegetative development of plant seedlings and to the various expressions of organogenesis were measured. Weak concentrations in sodium and calcium ions as well as a weak concentration in Ca++ (0.1 mM) with 50 mM in Na+ support the best vegetative development of the plantlets. The most important percentage of plant seedlings presenting a bud initiation was obtained on a medium containing 0.1 mM of Na+ and 50 mM of Ca++. Our study defined several media likely to support in vitro development of Prosopis farcta plantlets allowing the selection of salt tolerant plants or cellular lines. Some other media were chosen for improving micropropagation of the species without adding growth substances.

  8. Memory immune response and safety of a booster dose of Japanese encephalitis chimeric virus vaccine (JE-CV) in JE-CV-primed children.

    Science.gov (United States)

    Feroldi, Emmanuel; Capeding, Maria Rosario; Boaz, Mark; Gailhardou, Sophia; Meric, Claude; Bouckenooghe, Alain

    2013-04-01

    Japanese encephalitis chimeric virus vaccine (JE-CV) is a licensed vaccine indicated in a single dose administration for primary immunization. This controlled phase III comparative trial enrolled children aged 36-42 mo in the Philippines. 345 children who had received one dose of JE-CV in a study two years earlier, received a JE-CV booster dose. 105 JE-vaccine-naïve children in general good health were randomized to receive JE-CV (JE-vaccine naïve group; 46 children) or varicella vaccine (safety control group; 59 children). JE neutralizing antibody titers were assessed using PRNT50. Immunological memory was observed in children who had received the primary dose of JE-CV before. Seven days after the JE-CV booster dose administration, 96.2% and 66.8% of children were seroprotected and had seroconverted, respectively, and the geometric mean titer (GMT) was 231 1/dil. Twenty-eight days after the JE-CV booster dose seroprotection and seroconversion were achieved in 100% and 95.3% of children, respectively, and the GMT was 2,242 1/dil. In contrast, only 15.4% of JE-CV-vaccine naïve children who had not received any prior JE vaccine were seroprotected seven days after they received JE-CV. One year after receiving the JE-CV booster dose, 99.4% of children remained seroprotected. We conclude that JE-CV is effective and safe, both as a single dose and when administrated as a booster dose. A booster dose increases the peak GMT above the peak level reached after primary immunization and the antibody persistence is maintained at least one year after the JE-CV booster dose administration. Five year follow up is ongoing.

  9. Different Views of the Grand Canyon

    Science.gov (United States)

    Elders, Wilfred A.

    Each year the spectacular scenery of the Grand Canyon of Arizona awes its more than 4,000,000 visitors. Just as its enormous scale dwarfs our human sense of space, its geology also dwarfs our human sense of time. Perhaps here, more than anywhere else on the planet, we can experience a sense of ``Deep Time.'' The colorful rocks exposed in the vertical walls of the canyon display a span of 1.8 billion years of Earth's history [Beus and Morales, 2003]. But wait! There is a different view! According to Vail [2003], this time span is only 6,000 years and the Grand Canyon and its rocks are a record of the Biblical 6 days of creation and Noah's flood. During a visit to Grand Canyon, in August 2003, I learned that Vail's book, Grand Canyon: A Different View, is being sold within the National Park. The author and compiler of Grand Canyon: A Different View is a Colorado River guide who is well acquainted with the Grand Canyon at river level. He has produced a book with an attractive layout and beautiful photographs. The book is remarkable because it has 23 co-authors, all male, who comprise a veritable ``Who's Who'' in creationism. For example, Henry Morris and John Whitcomb, the authors of the seminal young Earth creationist text, The Genesis Flood [Whitcomb and Morris, 1961], each contribute a brief introduction. Each chapter of Grand Canyon: A Different View begins with an overview by Vail, followed by brief comments by several contributors that ``have been peer reviewed to ensure a consistent and Biblical perspective.'' This perspective is strict Biblical literalism.

  10. Somatic Embryogenesis Cocoa Plantlets

    Directory of Open Access Journals (Sweden)

    Teguh man santoso

    2009-12-01

    Full Text Available AbstractPratylenchus coffeae is a parasitic nematoda that infected the roots of some plants, one of them is coffee. The Infection of Pratylenchus coffeae cause root tissue damage that led to root lession and make root become rotten, it will interfere the ability of roots to absorb water and nutrients in the soil which resulted in the growth of plants. At the moment, control of Pratylenchus coffeae are following integrated pests management (IPM program, which integrated between the use of coffee resistant clone and application of biological agents. Research on biological control was conducted more intensive, at the moment; due to it is friendlier save against environment and cheaper then using chemical nematicides. The research was conducted to know the effects of Micorrhiza Helper Bacteria (MHB,Pseudomonas diminuta and Bacillus subtilis in suppressing the population of P. coffeaeas well as their effect on growth of coffee seedling.  Coffee arabica (Coffea arabica L. seedling one moth old were used in the experiment. The experiment prepared with eight treatments and five  replications, as follows: A (Pseudomonas diminuta with density of 108 cfu / ml, B (Pseudomonas diminuta with density of 2x108 cfu / ml, C (Bacillus subtilis with density of 108 cfu / ml , D (Bacillus subtilis with density 2x108 cfu / ml, E (Carbofuran nematicide 5 g formulation / pot, F (Pseudomonas diminuta and Bacillus subtilis with each density of 108 cfu / ml, K- (Nematoda inoculation but without bacteria and nematicide, K+ (coffee seedling  without any additional treatment. The experiment was conducted for sixteen weeks or about four months. The results of the experiment showed that application of MHB could suppress population of P. coffeae and increase coffee seedling growth significantly. Inoculation of B. subtilis at 108 cfu per seedling suppressed significantly nematoda population of 71.3% compared with untreated seedling but inoculated with nematoda. It was not significant different with carbofuran treatment which could suppress nematoda population by 89.7%. The same result also occur on the treatment of P. diminuta at density level 2.108 cfu/seedling, which could suppress nematoda population by 64.2%. Seedling growth were treated with MHB also significantly increase compared with seedling without treatment and inoculation of nematodas, especially on the treatment of B.subtilis at density level 108 cfu and P.diminuta at density level of 108 cfu, with increasing level of 35.4% and 34.2 %, respectively.Keywords: MHB (Mycorrhiza Helper Bacteria, Arabica coffee (Coffea arabica L.,  Pratylenchus coffeae, Bacillus subtilis, Pseudomonas diminuta.

  11. Produção integrada e convencional de pêssegos cv. Marli Integrated and convetional production of peach cv. Marli

    Directory of Open Access Journals (Sweden)

    José Luis da Silva Nunes

    2004-12-01

    Full Text Available O cultivo de pessegueiros é uma atividade de grande importância econômica no Sul do Brasil, onde se destaca o Estado do Rio Grande do Sul como maior produtor brasileiro. Um dos aspectos mais importantes na produção de alimentos da atualidade é a redução no uso de agroquímicos, com menor contaminação do ambiente e riscos reduzidos de resíduos. Este trabalho visou a comparar os sistemas de Produção Convencional (PC e Integrada (PI de pêssegos e foi realizado no ano de 2001, no município de São Jerônimo - RS, latitude 30°05'52" S, longitude 51°39'08" W e altitude de 46 metros. Áreas de um pomar comercial da cv. Marli foram avaliadas em relação às principais práticas de manejo da planta e do solo, controle fitossanitário, aspectos econômicos, bem como à qualidade da fruta. Na área conduzida sob PI, foram utilizadas as práticas de manejo preconizadas pelas Normas de Produção Integrada de Pêssegos (NPIP e, na área conduzida no sistema de PC, as plantas foram manejadas de acordo com as práticas comumente utilizadas pelo produtor. A produção de pêssegos, em ambos os sistemas, não foi afetada. Na área de PI, houve menor número de pêssegos por planta; entretanto, as frutas apresentaram maior peso médio. A maioria dos pêssegos da PI foram classificados como CAT I (diâmetro superior a 57 mm. As frutas produzidas na PC são, na maioria, de CAT II (de 48 a 57 mm. A qualidade pós-colheita não apresentou diferenças em relação à acidez, firmeza e cor. Com base nestes resultados, podemos concluir que é possível produzir pêssegos de qualidade com produtividade no sistema de PI.The peach-trees cultivation is a very economic important activity in South of Brazil, where highlights the State of Rio Grande do Sul as the largest Brazilian producer. One of the most important aspects in actuality is the food prodution with reduction of agrochemicals components, reducing the risks of environment contamination. This work

  12. DESBASTE DE MUDAS TIPO FILHOTE DO ABACAXI CV. PÉROLA: 1. PRODUÇÃO E QUALIDADE DO FRUTO SLIPS THINNING FROM THE PINEAPPLE CV. PÉROLA: 1. PRODUCTION AND FRUIT QUALITY

    Directory of Open Access Journals (Sweden)

    VALMIR PEREIRA DE LIMA

    2001-12-01

    Full Text Available O abacaxizeiro Pérola tem como uma das suas principais características a geração de elevado número de mudas tipo filhote, formando um cacho na parte superior do pedúnculo. Os filhotes desenvolvem-se no mesmo período da formação do fruto. Em plantio comercial na região do litoral Norte da Bahia, foi desenvolvido este estudo com o objetivo de avaliar o efeito do desbaste de mudas tipo filhote sobre aspectos vegetativos e produtivos do abacaxi cv. Pérola, cultivado sob condições de sequeiro. Nesta primeira parte, serão apresentados os efeitos sobre a produção e a qualidade do fruto. Em delineamento em blocos completos ao acaso, com sete repetições, foram estudados seis tratamentos, sendo a testemunha, sem desbaste, e os seguintes cinco níveis de desbaste de mudas tipo filhote: 1 - todas as mudas desbastadas; 2 -mantidas duas mudas do lado do sol poente; 3 - mantidas quatro mudas, com desbaste, de cima para baixo; 4 - mantidas quatro mudas, com desbaste, de baixo para cima; 5 - mantidas seis mudas. Por meio da análise de variância e teste de comparação entre as médias, foram avaliados aspectos da qualidade química e física dos frutos, tais como sólidos solúveis totais (SST, acidez total titulável (ATT, relação SST/ATT, peso do fruto com coroa, comprimento do fruto, peso e comprimento da coroa, produtividade, número e o peso médio dos frutos. O desbaste não determinou diferenças estatísticas para a produtividade da cultura e o peso do fruto, observando-se, no entanto, tendência consistente para a elevação dos valores deste último. O desbaste de mudas não afetou a qualidade do fruto, que apresentou características químicas, físicas e físico-químicas dentro dos padrões da cultivar Pérola.One of the main characteristics of the pineapple cv. Pérola is the generation of numerous plantlets called slips located at the upper part of the peduncle. The slips develop at the same period of fruit formation. In a

  13. Fruit size and quality of pineapples cv. Vitória in response to micronutrient doses and way of application and to soil covers

    Directory of Open Access Journals (Sweden)

    Aiala Vieira Amorim

    2011-10-01

    Full Text Available The objective of this study was to evaluate the effects of foundation and leaf fertilization with micronutrients on fruit size and quality of pineapple cv. Vitória under the environmental conditions of the Baixo Acaraú irrigated perimeter in Northern Ceará State, Brazil, under two covers (bagana and black plastic of the sandy soil of low fertility. The experimental design was a randomized split blocks one with four levels of soil dressing and four levels of foliar fertilization, with five replications. Micronutrient soil dressing was studied as FTE-12 at doses of 0, 60, 120 and 180 kg ha-1. The four levels of foliar fertilization were: LF0 (without fertilizer, LF 1 (15 leaf fertilization, using the amount of 1158.75 g Fe ha-1, 844.65 g Mn ha-1, 391.5 g ha-1 Zn, 322.65 g ha-1 Cu and 216 g ha-1 B, LF2 (15 leaf fertilization, using twice the quantities of level LF1 and LF3 (15 leaf fertilization, using three times the amount of level LF1. At 13 months after planting the micropropagated plantlets was carried out the floral induction treatment and five months later the fruit harvest determining the following variables: fruit weight and median diameter, soluble solids content (SS and titratable acidity (TA. Both fruit weight and diameter increased with increasing doses of micronutrients applied to the soil and to the leaves, of plants grown both on bagana soil cover and plastic mulch. On the other hand fruit pulp quality was little affected by the treatments studied. There were a small increase of SS contents for plants grown on bagana soil cover and a small decrease of titratable acidity for those grown on plastic mulch, in both cases just in response to micronutrient foliar application.

  14. Effect of Sodium Cyanide on Wheat (Triticum durum cv. Altar and T. aestivum cv. Cumhuriyet)

    DEFF Research Database (Denmark)

    Gemici, Meliha; Karshenass, A.M.; Tan, Kit

    2008-01-01

    , the first set was used as the control and watered using ordinary bottled water sold commercially. The other five sets with T. durum cv. Altar and T. aestivum cv. Cumhuriyet seedlings were additionally watered with various concentrations of sodium cyanide, the test-quantity used being 10-50 mg/L. Growth...

  15. Medium Optimization for Potato Plantlet Culture%马铃薯试管苗快繁培养基的优化研究

    Institute of Scientific and Technical Information of China (English)

    刘海英

    2014-01-01

    The effect of mineral salt concentrationson potato plantlet growth were studied to solve the problems about the long reproductive cycle and low survival rate for the potato plantlet culture.Adopted multi-factor orthogonal experiment L9 (34) design was used to change the MS medium concentration of CaCl2,Ca(NO3)2,KH2PO4,KNO3 to screen the optimum concentration for potato tissue culture medium preparation.The results showed that CaCl2 0mmol/L,Ca(NO3) 2 20mmol/L,KH2PO4 6mmol/L,KNO3 18mmol/L was the best combination for the potato plantlet culture medium.%针对马铃薯试管苗快繁继代繁殖周期长和移栽后成活率低的实际问题,以马铃薯品种克新l号脱毒试管苗为试验材料,采用多因子正交试验L9(34)设计方法,以MS培养基中4种无机盐CaCl2 、Ca(NO3)2、KH2 PO4、KNO3为试验因素,研究不同无机盐浓度对马铃薯试管苗生长的影响.结果表明,在CaCl2浓度为0mmol/L、Ca(NO3)2浓度为20mmol/L、KH2PO4浓度为6mmol/L、KNO3浓度为18 mmol/L条件下,克新1号马铃薯试管苗的株高、茎粗、根系、茎叶鲜重生长达到最好.本研究结果可为马铃薯组培快繁过程中培养基制备提供依据.

  16. Comparison of tracking algorithms implemented in OpenCV

    Directory of Open Access Journals (Sweden)

    Janku Peter

    2016-01-01

    Full Text Available Computer vision is very progressive and modern part of computer science. From scientific point of view, theoretical aspects of computer vision algorithms prevail in many papers and publications. The underlying theory is really important, but on the other hand, the final implementation of an algorithm significantly affects its performance and robustness. For this reason, this paper tries to compare real implementation of tracking algorithms (one part of computer vision problem, which can be found in the very popular library OpenCV. Moreover, the possibilities of optimizations are discussed.

  17. OpenCV 3.0 computer vision with Java

    CERN Document Server

    Baggio, Daniel Lélis

    2015-01-01

    If you are a Java developer, student, researcher, or hobbyist wanting to create computer vision applications in Java then this book is for you. If you are an experienced C/C++ developer who is used to working with OpenCV, you will also find this book very useful for migrating your applications to Java. All you need is basic knowledge of Java, with no prior understanding of computer vision required, as this book will give you clear explanations and examples of the basics.

  18. Las cinco grandes dimensiones de la personalidad

    OpenAIRE

    Jan ter Laak

    1996-01-01

    Este artículo revisa las distintas posiciones teóricas sobre las cinco grandes dimensiones de la personalidad, mostrando las semejanzas y diferencias entre las posturas teóricas. Esta contribución presenta lo siguiente: (a) la génesis del contenido y la estructura de las cinco dimensiones; (b) la fortaleza de las cinco dimensiones; (e) la relación de las cinco grandes dimensiones con otros constructos de personalidad; (d) discute el valor predictivo de las puntuaciones del perfil de las cinco...

  19. AVALIAÇÃO DA CV. CABERNET FRANC PARA ELABORAÇÃO DE VINHO TINTO EVALUATION OF CV. CABERNET FRANC TO ELABORATE RED WINE

    Directory of Open Access Journals (Sweden)

    Luiz Antenor RIZZON

    2001-08-01

    Full Text Available A uva Cabernet Franc, originária da região de Bordeaux, França, foi introduzida no Rio Grande do Sul através da Estação Agronômica de Porto Alegre. É utilizada para a elaboração de vinho tinto para ser consumido jovem, embora apresente aptidão para envelhecer. Face a sua importância, conduziu-se este trabalho com o objetivo de determinar as características agronômicas e enológicas da uva Cabernet Franc para elaboração de vinho tinto. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho tinto nas safras de 1987 a 1994. Os resultados obtidos evidenciaram que a cv. Cabernet Franc tem cacho médio, formado por bagas pequenas. O mosto possui teores de açúcar e de acidez adequados para vinificação. O vinho apresenta elevado teor de K e de álcoois superiores. No aspecto sensorial, apresenta cor vermelho-rubi, com reflexos violáceos quando jovem, e de intensidade variável em função das safras vitícolas. No olfato, apresenta aroma com notas vegetais e frutadas, que lembram pimentão e frutas vermelhas, respectivamente. Gustativamente, o vinho apresenta boa estrutura, equilíbrio e personalidade marcante.Cabernet Franc grape is originated from Bordeaux, France. It was introduced in the Serra Gaúcha region in the south of Brazil, by the Estação Agronômica the Porto Alegre, RS. It is used to elaborate young red wine, but it can be aged too. Due to the importance of Cabernet Franc, this work had the objective to determine its agronomic and winemaking characteristics. To achieve this purpose, variables related to the berries, clusters, must, and wine were performed from 1987 to 1994. The results showed that Cabernet Franc grapes have clusters of medium size and small berries. The must has adequate sugar and titratable acidity levels for vinification. The Cabernet Franc wine has a high concentration of K and higher alcohols. The sensory evaluation showed that it has a ruby color with violet reflexes when

  20. Grand Forks - East Grand Forks Urban Water Resources Study. Wastewater Management Appendix.

    Science.gov (United States)

    1981-07-01

    denitrification or clinoptilolite ion exchange is required to meet the ammonia and total nitrogen levels. The effluent from these unit processes would be filtered...32 45 Dissolved Oxygen (mg/1) At Grand Forks 6.0 7.0 8.6 i0.0 12.1 At East Grand Forks 6.5 7.4 9.0 11.4 12.7 Ammonia Nitrogen (mg/i) At Grand Forks...Concentration (mg/i) Total Solids 700 Dissolved Solids 500 Suspended Solids 200 BOD5 200 COD 500 Total Nitrogen 40 Organic Nitrogen 15 Ammonia Nitrogen

  1. Occurrence of Zaprionus indianus (Diptera: Drosophilidae in Agudo, Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Mauricio Batistella Pasini

    2012-03-01

    Resumo. Este trabalho faz menção ao primeiro registro de Zaprionus indianus Gupta (Diptera: Drosophilidae encontrado na zona rural do município de Agudo, no estado do Rio Grande do Sul, Brasil. Os adultos da mosca foram encontrados primeiramente em frutos de ameixa (Prunus salicina Lindl posteriormente em figos maduros (Ficus carica L. em dois pomares. No primeiro pomar cerca de 80% dos figos coletados apresentaram ataque de Z. indianus e, no segundo pomar 50% dos figos da cv. “Pingo de mel” e 80% da variedade “Roxo de Valinhos” foram infestados. No período correspondente a emergência dos adultos, coletou-se um total de 1364 indivíduos. Os figos da cv “Roxo de Valinhos” apresentaram maior emergência de adultos. Além de estar presente em restos culturais de figo, Z. indianus foi visualizada sobrevoando restos culturais de Syagrus romanzoffiana (Cham., Cucumis melo L., Citrullus vulgaris Schrad. e Vitis vinifera L., associada a outros drosofilídeos. Ressalta-se que medidas de monitoramento e controle da praga deverão ser adotadas no município para garantir figos de alta qualidade e sadios.

  2. Perancangan Kemasan Transportasi Buah Jambu Air (Syzygium aqueum cv Camplong

    Directory of Open Access Journals (Sweden)

    Iswahyudi

    2015-04-01

    Full Text Available Jamboo cv Camplong was an exotic fruit from Sampang Indonesia which had high economic values. The quality of fresh Jamboo was greatly influenced by the types of packaging and ways of its transportation that affected its shelf life. The purpose of this research were designing a primary packaging and analyzing the quality of Jamboo cv Camplong after short transportation (from Sampang to Surabaya. Farmers used conventional packaging with capacity of 8.4 kg/box (dimension 478 mm x 146 mm x 354 mm. Based on theoretical packaging design showed that the flute BC cardboard (capacity 4.5 kg/box, dimension 357 mm x 217 mm x 216 mm with partition flute A cardboard. In fact, there was needed modification of the dimension (342 mm x 210 mm x 200 mm because of the fruits diameter (60-65 mm. This result did not change the efficiency usage of transportation space (91-95% and compression strength of box can support (7 boxes/ stack. The result showed that the mechanical damage after transportation were 20.87% for conventional packaging (as control and 7.70% for modification packaging design (packaging with partition.

  3. Production Management in SME's Industry: Case Study of CV Wiracana

    Directory of Open Access Journals (Sweden)

    Apriani Kurnia Suci

    2015-08-01

    Full Text Available Production Management is the act of designing, operating, and improving the productive systems – a system for getting the work done. Along the process, the synergy in between machineries, facilities and people could empower the sustainable of the production. In fact, for some cases, the implementation of unique system is needed for the production process. The example for this transformation production management is in the case of CV Wiracana, a handmade manufacturing company for folding hand fans from Bali. CV Wiracana's products are very unique, combined from mass production for the speed and an art for the custom made product. At one side, the market forces them to speed up the production and for this purpose, they must set up the new system on their production line. On the other side, the masterpiece also needs to be produced without jeopardizing mass production line schedule. The transformation production system needs to be done no later than 2015 as the urgency to fulfill the customer demand, business growth, compete in the industry and sustainability. The changes are expected to improve the production at least about 20% or doubled from the current production.

  4. High efficiency transformation of banana [Musa acuminata L. cv. Matti (AA)] for enhanced tolerance to salt and drought stress through overexpression of a peanut salinity-induced pathogenesis-related class 10 protein.

    Science.gov (United States)

    Rustagi, Anjana; Jain, Shalu; Kumar, Deepak; Shekhar, Shashi; Jain, Mukesh; Bhat, Vishnu; Sarin, Neera Bhalla

    2015-01-01

    Bananas and plantains (Musa spp. L.) are important subsistence crops and premium export commodity in several countries, and susceptible to a wide range of environmental and biotic stress conditions. Here, we report efficient, rapid, and reproducible Agrobacterium-mediated transformation and regeneration of an Indian niche cultivar of banana [M. acuminata cv. Matti (AA)]. Apical meristem-derived highly proliferative multiple shoot clump (MSC) explants were transformed with the Agrobacterium strain EHA105 harboring a binary vector pCAMBIA-1301 carrying hptII and uidA. Sequential agro-infiltration (10 min, 400 mmHg), infection (additional 35 min, Agrobacterium density A 600 = 0.8) and co-cultivation (18 h) regimen in 100 µM acetosyringone containing liquid medium were critical factors yielding high transformation efficiency (~81 %) corroborated by transient GUS expression assay. Stable transgenic events were recovered following two cycles of meristem initiation and selection on hygromycin containing medium. Histochemical GUS assay in several tissues of transgenic plants and molecular analyses confirmed stable integration and expression of transgene. The protocol described here allowed recovery of well-established putative transgenic plantlets in as little as 5 months. The transgenic banana plants could be readily acclimatized under greenhouse conditions, and were phenotypically similar to the wild-type untransformed control plants (WT). Transgenic plants overexpressing Salinity-Induced Pathogenesis-Related class 10 protein gene from Arachis hypogaea (AhSIPR10) in banana cv. Matti (AA) showed better photosynthetic efficiency and less membrane damage (P < 0.05) in the presence of NaCl and mannitol in comparison to WT plants suggesting the role of AhSIPR10 in better tolerance of salt stress and drought conditions.

  5. Early evaluation and on field conditions of resistance to Mycosphaerella fijiensis Morelet of plants from Grande naine (AAA cultivar, obtained through out tissue culture and mutations induction

    Directory of Open Access Journals (Sweden)

    Lourdes R. García

    2003-04-01

    Full Text Available The present work was carried out in the Plants Biotechnology Institute of the Central University of Las Villas. The plant material from the cv. Grande Naine (AAA was treated with physical mutagenic agents(gamma radiation 60Co source to induce genetic variability. The behaviour of the population to the black Sigatoka was evaluated. A somaclone was selected by its disease resistance and was in vitro multiplied and the plants were acclimatized to evaluate its behaviour facing the disease on greenhouse conditions and in a second cycle of multiplication in the field. The results showed that in the majority of the plants were not found differences respect cv Grande Naine, just one presented similar reaction to cv. ‘FHIA 18’ (AAAB (partially resistant as for the variable evaluated, being obtained a frequency of 0.018% for this character. This plant was named IBP 446. After 60 days of application of the mycelial homogenized of M. fijiensis in micropropagated plants of this somaclone, differences in the respect affectation states were found at susceptible witness in greenhouse conditions. When plants of the IBP 446 were evaluated in a second cycle of multiplication differences were found with the susceptible control only at flowering, while they behaved similar at susceptible control in the crop. Key words: early detection, breeding, mutation, Black Sigatoka

  6. Plasma nanoscience: setting directions, tackling grand challenges

    Science.gov (United States)

    (Ken Ostrikov, Kostya; Cvelbar, Uros; Murphy, Anthony B.

    2011-05-01

    This review paper presents historical perspectives, recent advances and future directions in the multidisciplinary research field of plasma nanoscience. The current status and future challenges are presented using a three-dimensional framework. The first and the largest dimension covers the most important classes of nanoscale objects (nanostructures, nanofeatures and nanoassemblies/nanoarchitectures) and materials systems, namely carbon nanotubes, nanofibres, graphene, graphene nanoribbons, graphene nanoflakes, nanodiamond and related carbon-based nanostructures; metal, silicon and other inorganic nanoparticles and nanostructures; soft organic nanomaterials; nano-biomaterials; biological objects and nanoscale plasma etching. In the second dimension, we discuss the most common types of plasmas and plasma reactors used in nanoscale plasma synthesis and processing. These include low-temperature non-equilibrium plasmas at low and high pressures, thermal plasmas, high-pressure microplasmas, plasmas in liquids and plasma-liquid interactions, high-energy-density plasmas, and ionized physical vapour deposition as well as some other plasma-enhanced nanofabrication techniques. In the third dimension, we outline some of the 'Grand Science Challenges' and 'Grand Socio-economic Challenges' to which significant contributions from plasma nanoscience-related research can be expected in the near future. The urgent need for a stronger focus on practical, outcome-oriented research to tackle the grand challenges is emphasized and concisely formulated as from controlled complexity to practical simplicity in solving grand challenges.

  7. "Teine" võitis Prantsusmaal Grand Prix'

    Index Scriptorium Estoniae

    2006-01-01

    Prantsusmaal Essonne'is toimuval 8. Euroopa filmifestivalil Cinessonne sai üliõpilaste žürii grand prix rahvusvahelises ühistöös valminud tantsufilm "Teine" ("Another") : režissöör Rene Vilbre. Ka teistest festivalidest, kus film osalenud

  8. Youth Voice and the Llano Grande Center

    Science.gov (United States)

    Guajardo, Francisco; Perez, Delia; Guajardo, Miguel A.; Davila, Eric; Ozuna, Juan; Saenz, Maribel; Casaperalta, Nadia

    2006-01-01

    The Llano Grande Center is a non-profit education and community development organization founded in the mid-1990s by youth and teachers out of a public high school classroom in a rural South Texas (USA) community. The Center was created, in large part, to cultivate youth voices as important elements of curriculum development and teacher training…

  9. Summer Grand Summons Ceremony in Tashilhungpo Monastery

    Institute of Scientific and Technical Information of China (English)

    XILHU

    2003-01-01

    Every year, Tashilhungpo Monastery celebrates the Summer Grand Summons Ceremony (called Ximoqenbo in Tibetan) in the eighth month of the Tibetan calendar. All the monks and lay people of the Xigaze area get together to watch Chammo, which is a kind of sorcerer's dance. It is also a Tantric ritual

  10. Substance Abuse in the Rio Grande Valley.

    Science.gov (United States)

    Zavaleta, Anthony N.

    1979-01-01

    In the Mexican American barrios of Texas' Lower Rio Grande Valley, existence is complicated by the interactive forces of culture, society, and economy. These three factors act in unison to create an etiology of alcohol and drug use and abuse which is poorly understood by persons outside the barrio's grasp. (Author/NQ)

  11. A Grand Unified Theory of Interdisciplinarity

    Science.gov (United States)

    Davis, Lennard J.

    2007-01-01

    Aside from the appeal to administrators as a tool to reduce costs by combining less robust departments with heftier relations, interdisciplinarity is a powerful idea because it implies that different branches of knowledge can benefit from talking to one another: a grand, unified theory of knowledge in which each discipline contributes building…

  12. Salinity management in the Rio Grande Bosque

    Science.gov (United States)

    Jan M. H. Hendrickx; J. Bruce J. Harrison; Jelle Beekma; Graciela Rodriguez-Marin

    1999-01-01

    This paper discusses management options for salinity control in the Rio Grande Bosque. First, salt sources are identified and quantified. Capillary rise of ground water is the most important cause for soil salinization in the bosque. Next, a riparian salt balance is presented to explain the different mechanisms for soil salinization. Finally, the advantages and...

  13. Reisipakkumine - Grand Tour Itaalias / Mai Levin

    Index Scriptorium Estoniae

    Levin, Mai, 1942-

    2009-01-01

    Tiina Abeli koostatud ja Urmas Viigi kujundatud näitus "Grand Tour. Eesti kunstnikud Itaalias" Kumu Kunstimuuseumis 05. aprillini. Loetletud eksponeeritud tööde autoreid. Näitus annab ülevaate, kes siinsetest kunstnikest 19. sajandi algusest kuni 1930ndate aastateni Itaalias käisid ja kuidas see nende loomingut mõjutas

  14. The Virtual Grand Tour as Educational Paradigm

    DEFF Research Database (Denmark)

    Hansen, Per Skafte; Mouritsen, Lars

    2001-01-01

    The Virtual Grand Tour as defined here bears some resemblance to its 18th century ancestor: a wide range of individual topics are treated as a whole; a tutor, whether real or simulated, present or remote, is provided; a set of problem solving tools forms an integrated part of the "traveller's" eq...

  15. Radiatively Induced Fermi Scale in Grand Unification

    DEFF Research Database (Denmark)

    Alanne, Tommi; Meroni, Aurora; Sannino, Francesco;

    2016-01-01

    We consider Grand Unified Theories in which the hierarchy between the unification and the Fermi scale emerges radiatively. Within the Pati-Salam framework, we show that it is possible to construct a viable model where the Higgs is an elementary pseudo-Goldstone boson, and the correct hierarchy...

  16. The Virtual Grand Tour as Educational Paradigm

    DEFF Research Database (Denmark)

    Hansen, Per Skafte; Mouritsen, Lars

    2001-01-01

    The Virtual Grand Tour as defined here bears some resemblance to its 18th century ancestor: a wide range of individual topics are treated as a whole; a tutor, whether real or simulated, present or remote, is provided; a set of problem solving tools forms an integrated part of the "traveller...

  17. Reisipakkumine - Grand Tour Itaalias / Mai Levin

    Index Scriptorium Estoniae

    Levin, Mai, 1942-

    2009-01-01

    Tiina Abeli koostatud ja Urmas Viigi kujundatud näitus "Grand Tour. Eesti kunstnikud Itaalias" Kumu Kunstimuuseumis 05. aprillini. Loetletud eksponeeritud tööde autoreid. Näitus annab ülevaate, kes siinsetest kunstnikest 19. sajandi algusest kuni 1930ndate aastateni Itaalias käisid ja kuidas see nende loomingut mõjutas

  18. Grand Canyon Humpback Chub Population Improving

    Science.gov (United States)

    Andersen, Matthew E.

    2007-01-01

    The humpback chub (Gila cypha) is a long-lived, freshwater fish found only in the Colorado River Basin. Physical adaptations-large adult body size, large predorsal hump, and small eyes-appear to have helped humpback chub evolve in the historically turbulent Colorado River. A variety of factors, including habitat alterations and the introduction of nonnative fishes, likely prompted the decline of native Colorado River fishes. Declining numbers propelled the humpback chub onto the Federal list of endangered species in 1967, and the species is today protected under the Endangered Species Act of 1973. Only six populations of humpback chub are currently known to exist, five in the Colorado River Basin above Lees Ferry, Ariz., and one in Grand Canyon, Ariz. The U.S. Geological Survey's Grand Canyon Monitoring and Research Center oversees monitoring and research activities for the Grand Canyon population under the auspices of the Glen Canyon Dam Adaptive Management Program (GCDAMP). Analysis of data collected through 2006 suggests that the number of adult (age 4+ years) humpback chub in Grand Canyon increased to approximately 6,000 fish in 2006, following an approximate 40-50 percent decline between 1989 and 2001. Increasing numbers of adult fish appear to be the result of steadily increasing numbers of juvenile fish reaching adulthood beginning in the mid- to late-1990s and continuing through at least 2002.

  19. Plasma nanoscience: setting directions, tackling grand challenges

    Energy Technology Data Exchange (ETDEWEB)

    Ostrikov, Kostya [Plasma Nanoscience Centre Australia (PNCA), CSIRO Materials Science and Engineering, PO Box 218, Lindfield NSW 2070 (Australia); Cvelbar, Uros [Jozef Stefan Institute, 39 Jamova cesta, Ljubljana, SI-1000 (Slovenia); Murphy, Anthony B, E-mail: Kostya.Ostrikov@csiro.au, E-mail: Uros.Cvelbar@ijs.si, E-mail: Tony.Murphy@csiro.au [CSIRO Materials Science and Engineering, PO Box 218, Lindfield NSW 2070 (Australia)

    2011-05-04

    This review paper presents historical perspectives, recent advances and future directions in the multidisciplinary research field of plasma nanoscience. The current status and future challenges are presented using a three-dimensional framework. The first and the largest dimension covers the most important classes of nanoscale objects (nanostructures, nanofeatures and nanoassemblies/nanoarchitectures) and materials systems, namely carbon nanotubes, nanofibres, graphene, graphene nanoribbons, graphene nanoflakes, nanodiamond and related carbon-based nanostructures; metal, silicon and other inorganic nanoparticles and nanostructures; soft organic nanomaterials; nano-biomaterials; biological objects and nanoscale plasma etching. In the second dimension, we discuss the most common types of plasmas and plasma reactors used in nanoscale plasma synthesis and processing. These include low-temperature non-equilibrium plasmas at low and high pressures, thermal plasmas, high-pressure microplasmas, plasmas in liquids and plasma-liquid interactions, high-energy-density plasmas, and ionized physical vapour deposition as well as some other plasma-enhanced nanofabrication techniques. In the third dimension, we outline some of the 'Grand Science Challenges' and 'Grand Socio-economic Challenges' to which significant contributions from plasma nanoscience-related research can be expected in the near future. The urgent need for a stronger focus on practical, outcome-oriented research to tackle the grand challenges is emphasized and concisely formulated as from controlled complexity to practical simplicity in solving grand challenges.

  20. Discovery of the Grand Unified Theory

    Science.gov (United States)

    Nair, Radhakrishnan

    2010-11-01

    I have discovered the Grand Unified Theory which unites quantum with classical mechanics. This discovery is based on a geocentric universe, the myth of empty space, four states of matter in three dimensional space and space-time exponentiation, instead of space-time curvature.

  1. Feature selection with the image grand tour

    Science.gov (United States)

    Marchette, David J.; Solka, Jeffrey L.

    2000-08-01

    The grand tour is a method for visualizing high dimensional data by presenting the user with a set of projections and the projected data. This idea was extended to multispectral images by viewing each pixel as a multidimensional value, and viewing the projections of the grand tour as an image. The user then looks for projections which provide a useful interpretation of the image, for example, separating targets from clutter. We discuss a modification of this which allows the user to select convolution kernels which provide useful discriminant ability, both in an unsupervised manner as in the image grand tour, or in a supervised manner using training data. This approach is extended to other window-based features. For example, one can define a generalization of the median filter as a linear combination of the order statistics within a window. Thus the median filter is that projection containing zeros everywhere except for the middle value, which contains a one. Using the convolution grand tour one can select projections on these order statistics to obtain new nonlinear filters.

  2. Rio Branco, grand strategy and naval power

    Directory of Open Access Journals (Sweden)

    João Paulo Alsina Jr.

    2014-12-01

    Full Text Available This article addresses Baron of Rio Branco's grand strategy and the role played by the naval reorganization program (1904-1910 in this context. The ensuing case study determined the domestic and international constraints that affected the program, as well as the worldview of the patron of Brazilian diplomacy regarding military power's instrumentality to foreign policy.

  3. Qualidade de fruto da aceroleira cv. Olivier em dois estádios de maturação

    Directory of Open Access Journals (Sweden)

    Elisa Adriano

    2011-10-01

    Full Text Available A acerola é um fruto de grande potencial econômico e nutricional devido ao seu alto teor de vitamina C, destacando-se como alimento funcional. É comercializada principalmente na forma de polpa congelada e fruto in natura. O objetivo deste estudo foi avaliar a qualidade dos frutos da aceroleira cv. Olivier em dois estádios de maturação. Para tanto, foram colhidas amostras de frutos em um pomar comercial no município de Junqueirópolis-SP. Foram realizadas a determinação da cor externa dos frutos e análises das características químicas de teor de sólidos solúveis, pH, acidez titulável, açúcares redutores, 'ratio' e ácido ascórbico de frutos semi-maduros e maduros. Os resultados obtidos permitiram concluir que os frutos semimaduros apresentaram maior acidez total, menor teor de sólidos solúveis e menor concentração de açúcares; no entanto, estes frutos apresentaram maiores teores de vitamina C, expressa em ácido ascórbico. Portanto, quando se buscam altos índices de vitamina C, os frutos devem ser colhidos num estádio de maturação menos avançado, com coloração alaranjada. O estudo demonstrou também que a cv Olivier produz frutos com características adequadas tanto para o mercado in natura quanto para a indústria, apresentando boa coloração e características químicas dentro dos padrões para esta fruta.

  4. Creationism in the Grand Canyon, Texas Textbooks

    Science.gov (United States)

    Folger, Peter

    2004-01-01

    AGU President Bob Dickinson, together with presidents of six other scientific societies, have written to Joseph Alston, Superintendent of Grand Canyon National Park, pointing out that a creationist book, The Grand Canyon: A Different View, is being sold in bookstores within the borders of the park as a scientific explanation about Grand Canyon geologic history. President Dickinson's 16 December letter urges that Alston clearly separate The Grand Canyon: A Different View from books and materials that discuss the legitimate scientific understanding of the origin of the Grand Canyon. The letter warns the Park Service against giving the impression that it approves of the anti-science movement known as young-Earth creationism, or that it endorses the advancement of religious tenets disguised as science. The text of the letter is on AGU's Web site http://www.agu.org/sci_soc/policy/sci_pol.html. Also, this fall, AGU sent an alert to Texas members about efforts by intelligent design creationists aimed at weakening the teaching of biological evolution in textbooks used in Texas schools. The alert pointed scientists to a letter, drafted by AGU, together with the American Institute of Physics, the American Physical Society, the Optical Society of America, and the American Astronomical Society, that urged the Texas State Board of Education to adopt textbooks that presented only accepted, peer-reviewed science and pedagogical expertise. Over 550 scientists in Texas added their names to the letter (http://www.agu.org/sci_soc/policy/texas_textbooks.pdf ), sent to the Board of Education on 1 November prior to their vote to adopt a slate of new science textbooks. The Board voted 11-5 in favor of keeping the textbooks free of changes advocated by groups supporting intelligent design creationism.

  5. Morphogenetic characteristics in Tanzania grass conhsorted with Stylosanthes Campo Grande or fertilized with nitrogen under grazing

    Directory of Open Access Journals (Sweden)

    Túlio Otávio Jardim D'Almeida Lins

    2015-08-01

    Full Text Available This study aimed to study morphogenic and structural characteristics of Tanzania grass (Panicum maximum cv. Tanzania intercropped with Estilosantes Campo Grande (Stylosanthes capitata and Stylosanthes macrocephala or fertilized with nitrogen. The pasture was managed under continuous stocking and variable stocking rate. Were used a randomized complete blocks with split plots and three replications. The treatments were: Tanzania grass + Stylosanthes; Tanzania grass + 75 Kg N.ha. year-1; Tanzania grass + 150Kg N.ha.year-1; Tanzania grass + 225 Kg N.ha.year-1. Were used urea and ammonium nitrate as nitrogen source. The morphogenetic evaluations were conducted in the spring and summer. Were evaluated 15 tillers per paddock, twice a week for four weeks per season in study. The morphogenic characteristics were not affected by nitrogen fertilization or consortium, except the leaf elongation rate (LER. The highest values for this variable were observed in the spring in the fertilized pastures. Therefore, it is concluded that nitrogen fertilization influences the leaf elongation rate (LER of Tanzania grass, and this one when is intercropped with Stylosanthes Campo Grande show morphogenic characteristics similar when fertilized with nitrogen, except for rate leaf elongation.

  6. GpuCV : a GPU-accelerated framework for image processing and computer vision

    OpenAIRE

    ALLUSSE, Yannick; Horain, Patrick; Agarwal, Ankit; Saipriyadarshan, Cindula

    2008-01-01

    International audience; This paper presents briefly describes the state of the art of accelerating image processing with graphics hardware (GPU) and discusses some of its caveats. Then it describes GpuCV, an open source multi-platform library for GPU-accelerated image processing and Computer Vision operators and applications. It is meant for computer vision scientist not familiar with GPU technologies. GpuCV is designed to be compatible with the popular OpenCV library by offering GPU-accelera...

  7. Liberating methodological thinking in human sciences from grand theories

    DEFF Research Database (Denmark)

    Kharlamov, Nikita; Baldursson, Einar Baldvin

    2016-01-01

    focus on the necessity of a “grand unified theory” at the expense of any and all alternative perspectives. Properties of grand theories are discussed on the examples of Giddens and Bourdieu. It is argued that grand theories hamper a more productive focus on concrete phenomena. Robert Merton’s focus...

  8. 78 FR 39998 - Safety Zone; Grand Haven 4th of July Fireworks; Grand River; Grand Haven, MI

    Science.gov (United States)

    2013-07-03

    ... , type the docket number in the ``SEARCH'' box and click ``SEARCH.'' Click on Open Docket Folder on the... arc of a circle with an 800 foot radius with a center in position 43 3' 55.7'' N and 86 14' 13.8'' W...) Location. All waters of the Grand River within the arc of a circle with an 800 foot radius with a center...

  9. Pengaruh Budaya Organisasi, Kepemimpinan, dan Motivasi Kerja terhadap Kepuasan Kerja Karyawan CV Surya Raya

    Directory of Open Access Journals (Sweden)

    Laksmi Sito Dwi Irvianti

    2012-05-01

    Full Text Available This research was conducted on CV Surya Raya which is engaged in car shipping. The aim of this study is to determine the influence of organization culture, leadership, and working motivation partially to employees’ satisfaction in CV Surya Raya. The data was collected by conducting interviews with the company and distributing questionnaires to 67 employees as respondents and then analyzed using linear regression analysis method. From the research results, leadership variable does not have a significant impact on employees' job satisfaction in CV Surya Raya. Whereas, the motivation variable has greater influence on job satisfaction of CV Surya Raya employees compared to the organization's culture.

  10. Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel

    National Research Council Canada - National Science Library

    Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2012-01-01

    Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel...

  11. Herbs Recognition Based on Android using OpenCV

    Directory of Open Access Journals (Sweden)

    I Wayan Agus Suryawibawa

    2015-01-01

    Full Text Available Herbs are used in traditional medicine. There are so many herbs are spread across the world, it is difficult to memorize it all. This paper describes an android application to recognize herbs by their leaf characteristics (shape, veins, and keypoints. Shape and veins of leaves are recognized by Invariant Moment Method as the feature extraction. City Block Distance used to calculate the distance between the features. Whereas for detection and keypoints extraction using Oriented FAST and Rotated BRIEF on OpenCV library. This keypoints distance calculation using Brute-Force Hamming. Matching is done by calculating the shortest distance between test image and reference image. If the result is less than or equal to threshold then image is match. Experiment result show this application can achieve 79% of success rate by using keypoints. This result is influenced by glossy leaf surface, so there is many reflected light that become noise.

  12. OpenCV Based Disease Identification of Mango Leaves

    Directory of Open Access Journals (Sweden)

    Jayaprakash Sethupathy

    2016-10-01

    Full Text Available This paper aims in classifying and identifying the diseases of mango leaves for Indian agriculture. K-means algorithm is chosen for the disease segmentation, and the disease classification and identification is carried out using the SVM classifier. Disease identification based on analysis of patches or discoloring of leaf will hold good for some of the plant diseases, but some other diseases which will deform the leaf shape cannot be identified based on the same method. In this case leaf shape based disease identification has to be performed. Based on this analysis two topics are addressed in this research paper. (1 Disease identification using the OpenCV libraries (2 Leaf shape based disease identification.

  13. Propolis extract in postharvest conservation Solo papaya cv. 'Golden'

    Directory of Open Access Journals (Sweden)

    Flávia Regina Passos

    2016-12-01

    Full Text Available The high perishability of papaya (Carica papaya L. reduces its lifespan as well as limits marketing. Coating the fruits is an alternative to aid food preservation. We aimed to evaluate the effects of coating propolis extract on the physicochemical characteristics of papaya stored at room temperature. Solo papayas cv. 'Golden' were randomly divided into five postharvest treatment groups, three forms of dip-coating (70% alcohol, hydroalcoholic extract of propolis to 2.5%, and hydroalcoholic extract of propolis to 5% and two controls (one uncoated and one with refrigerated uncoated fruits. The weight loss, firmness, soluble solids (SS, titratable acidity (TA, SS/TA, and hydrogen potential (pH were evaluated at the intervals of 4 days every 12th storage day. Sensory analysis was performed on the 4th day of storage of papayas and evaluated by untrained through the acceptance testing. Treatments “refrigerated”, “propolis 2.5%”, and “5% propolis” presented with the lowest weight loss. The firmness level for treatment propolis 5% was superior to that of control, alcohol, and 2.5% propolis treatments. The SS was greater for propolis 5% treatment, which only differed from the alcohol treatment. The TA and SS/TA did not vary with the treatments, but with the storage time. The pH of refrigerated papaya showed significant differences in relation to other treatments. The fruits subjected to refrigerated treatment presented with chilling injury. Papayas coated with propolis extract showed sensory acceptability similar to that in other treatments on the 4th day of storage. The coating of propolis extract is a promising alternative for the control of weight loss and for the maintenance of firmness in Solo papaya cv. 'Golden'.

  14. Caracteres anatômicos de fibras foliares de brotações de curauá propagadas in vitro = Anatomical characters of curauá foliar fibers from plantlets in vitro propagation

    Directory of Open Access Journals (Sweden)

    Osmar Alves Lameira

    2007-01-01

    Full Text Available O curauá [Ananas erectifolius (L.B.Sm - Bromeliaceae] é uma espécie com grande potencial de utilização na indústria automobilística, devido à sua resistência, maciez e peso reduzido. O objetivo deste trabalho foi comparar fibras presentes em brotações de curauá cultivadas in vitro, advindas ou não do estiolamento, bem como contribuir para a descrição anatômica das folhas. Foram comparadas brotações de curauá produzidas in vitro, pelo método convencional (T1 e pelo método de estiolamento (T2. Nas seções transversais, os feixes de fibras associadas e não às nervuras foram contados e tiveram os diâmetros medidos. O número e o diâmetro dos feixes de fibras associadas ou não às nervuras foram superiores no (T1. Este tratamento produziu 23,7 fibras não associadas às nervuras, enquanto que o (T2 produziu 14,3, e nas fibras associadas às nervuras, o (T1produziu 14,6 e o (T2 produziu 11,3. No diâmetro das fibras associadas às nervuras em (T1 obteve-se 61,61 μm e em (T2, 53,17 μm. Para o diâmetro das fibras não associadas às nervuras, não houve diferença significativa. Quanto às características anatômicas, as maiores diferenças visuais foram constatadas no volume do clorênquima e do parênquima aqüífero, que foram maiores no (T2. Portanto, brotações de curauá cultivadas in vitro obtidas de brotos não estiolados possuem a tendência de produzir mais fibras.Curauá [Ananas erectifolius (L.B.Sm - Bromeliaceae] is a species with great utilization potential in the car manufacture, due to its resistance, softness and reduced weight. The objective of this study was to examine the presence of fibers in curauá plantlet cultured in vitro deriving or not from elongation as well as to contribute to anatomical leaf description. curauá shoots produced in vitro were compared, by the conventional method (T1 and by the elongation method (T2. In the transversal cuts, fibers bundle related or notto the nervure were

  15. The Grand Tack model: a critical review

    CERN Document Server

    Raymond, Sean N

    2014-01-01

    The `Grand Tack' model proposes that the inner Solar System was sculpted by the giant planets' orbital migration in the gaseous protoplanetary disk. Jupiter first migrated inward then Jupiter and Saturn migrated back outward together. If Jupiter's turnaround or "tack" point was at ~1.5 AU the inner disk of terrestrial building blocks would have been truncated at ~1 AU, naturally producing the terrestrial planets' masses and spacing. During the gas giants' migration the asteroid belt is severely depleted but repopulated by distinct planetesimal reservoirs that can be associated with the present-day S and C types. The giant planets' orbits are consistent with the later evolution of the outer Solar System. Here we confront common criticisms of the Grand Tack model. We show that some uncertainties remain regarding the Tack mechanism itself; the most critical unknown is the timing and rate of gas accretion onto Saturn and Jupiter. Current isotopic and compositional measurements of Solar System bodies -- including ...

  16. Status of the KASCADE-Grande experiment

    Energy Technology Data Exchange (ETDEWEB)

    Ulrich, H. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany)], E-mail: ulrich@ik.fzk.de; Apel, W.D.; Arteaga, J.C.; Badea, A.F.; Bekk, K. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Bercuci, A. [National Institute of Physics and Nuclear Engineering, P.O. Box Mg-6, RO-7690 Bucharest (Romania); Bertaina, M. [Dipartimento di Fisica Generale dell' Universita, 10125 Torino (Italy); Bluemer, J. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Institut fuer Experimentelle Kernphysik, Universitaet Karlsruhe, D-76021 Karlsruhe (Germany); Bozdog, H. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Brancus, I.M. [National Institute of Physics and Nuclear Engineering, P.O. Box Mg-6, RO-7690 Bucharest (Romania); Brueggemann, M.; Buchholz, P. [Fachbereich Physik, Universitaet Siegen, 57068 Siegen (Germany); Chiavassa, A. [Dipartimento di Fisica Generale dell' Universita, 10125 Torino (Italy); Cossavella, F. [Institut fuer Experimentelle Kernphysik, Universitaet Karlsruhe, D-76021 Karlsruhe (Germany); Daumiller, K. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Di Pierro, F. [Dipartimento di Fisica Generale dell' Universita, 10125 Torino (Italy); Doll, P.; Engel, R.; Engler, J. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Finger, M. [Institut fuer Experimentelle Kernphysik, Universitaet Karlsruhe, D-76021 Karlsruhe (Germany)] (and others)

    2008-01-15

    The KASCADE-Grande experiment measures extensive air showers induced by cosmic rays in the energy range between 0.5 PeV and 1 EeV - the so-called knee region. Principal task of the experiment is to measure with high accuracy the energy and composition of primary cosmic rays to shed light on the nature of the knee. The data of the former KASCADE experiment have been used in a composition analysis showing the knee at 3-5 PeV to be caused by a steepening in the light-element spectra. In the following, an update on these analyses will be given. In addition, the status of the experimental extension - the Grande array - together with first results will be briefly discussed.

  17. Splendid Culture on the Grand Canal

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    JINING Prefecture on thesouthwest plain of ShandongProvince is known as the homeof Confucius and Mencins and theland of etiquette.It is famous as thebirthplace or residence of many out-standing people and has beautifulscenery with blue Weishan Lake tothe south,lofty Mount Tai to thenorth and Liang Mountain by thelake in the west.The Beijing-Hangzhor Grand Canal crosses theprefecture,which has been a com-munications hub between north andsouth China since the Ming

  18. Kocel Steel Foundry Grand Opening Ceremony

    Institute of Scientific and Technical Information of China (English)

    TIAN Shi-jiang

    2006-01-01

    @@ On May 9, 2006 the Grand Opening ceremony of Kocel Steel Foundry Co., Ltd. was held in the National Economic and Technological Development Zone in Yinchuan City, Ningxia, China. Kocel Steel Foundry Co.,Ltd. is a large scale enterprise producing steel castings and it is jointly established by Ningxia Kocel Group,Changcheng Suzaki Machine & Foundry Co., Ltd. and Voestalpine Giesserei Linz GmbH, Austria.

  19. The Internet in developing nations: Grand challenges

    OpenAIRE

    Press, Larry

    2004-01-01

    This is a call for a "Grand Challenge" project for achieving truly global connectivity. For over a decade, we have hypothesized that the Internet could raise the quality of life in developing nations. We have conducted hundreds of studies of the state of the Internet and "e–readiness," done extensive training of technicians and policy makers, run pilot studies, and held local, regional and global conferences and workshops. After all of this activity, Internet connectivity is nearly non–existe...

  20. In vitro organogenesis of zucchini squash cv. Caserta Organogênese in vitro de abobrinha-de-moita cv. Caserta

    Directory of Open Access Journals (Sweden)

    Liliane Cristina L Stipp

    2012-06-01

    Full Text Available A protocol for the in vitro culture of Cucurbita pepo cv. Caserta was studied, using a cotyledon segment with an attached hypocotyl fragment as an explant. First, to determine the optimal seedling age, explants were collected from 4 to 6-day-old in vitro germinated seedlings and cultured in MS basal medium supplemented with benzylaminopurine (BAP, 4.5 µM, under a 16-h photoperiod at 27ºC. Based on the results obtained, the explants collected from the 4-day-old seedlings were then cultured in MS basal medium supplemented with different concentrations of BAP (0, 1.1, 2.2, 3.3, 4.5, or 5.5 µM and incubated under a 16-h photoperiod at 27ºC. In vitro organogenesis was most efficient with explants collected from 4-day-old seedlings cultured in medium supplemented with 4.5 µM of BAP. After 4 weeks of incubation the development of adventitious buds at the cotyledon/hypocotyl junction could be observed. These buds were transferred to elongation and rooting medium and the developed plants were acclimatized to greenhouse conditions. The morphogenic process was characterized using light and scanning electron microscopy analyses to confirm the organogenesis. The results showed that this alternate explant is efficient for in vitro culture of zucchini squash cv. Caserta. The protocol will be further examined for future use in genetic transformation experiments in this species.O objetivo do trabalho foi estudar um protocolo para o cultivo in vitro de Cucurbita pepo cv. Caserta utilizando como explante um segmento de cotilédone associado a um fragmento do hipocótilo. Explantes foram coletados de plântulas germinadas in vitro com 4 a 6 dias de idade, cultivados em meio de cultura basal MS suplementado com benzilaminopurina (BAP, 4,5 µM, e incubados sob fotoperíodo de 16 h, a 27ºC. Com base nos resultados obtidos, explantes coletados de plântulas germinadas in vitro com 4 dias de idade foram cultivados em meio de cultura MS, suplementados com

  1. Gravity as a Grand Unification of Forces

    CERN Document Server

    Sharafiddinov, R S

    2004-01-01

    Any of all possible types of charges corresponds in nature to a kind of the inertial mass. Such a mass - charge duality of matter explains the coexistence of grand united rest mass and charge for the same neutrino equal respectively to its all the gravitational mass and charge which consist of the gravitoelectric, gravitoweak, gravitostrong and a range of others, innate components. From their point of view, a new grand unification theory has been created at the discussion of a question about unification of forces of a different nature. In this theory, the gravitational field must be naturally united gauge field of the unified system of the most diverse combinations of electromagnetic photons, weak bosons and strong gluons where the four pairs of forces of the micro world fundamental interactions are united. Some consequences and laboratory confirmations of the suggested theory have been listed which allow also to define the structure of the graviton as a grand united boson. Thereby it gives the possibility to...

  2. Raptor Use of the Rio Grande Gorge

    Energy Technology Data Exchange (ETDEWEB)

    Ponton, David A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-03-20

    The Rio Grande Gorge is a 115 km long river canyon located in Southern Colorado (15 km) and Northern New Mexico (100 km). The majority of the canyon is under the administration of the Bureau of Land Management {BLM), and 77 km of the canyon south of the Colorado/New Mexico border are designated Wild River under the National Wild and Scenic Rivers Act of 1968. Visits I have made to the Rio Grande Gorge over the past 15 .years disclosed some raptor utilization. As the Snake River Birds of Prey Natural Area gained publicity, its similarity to the Rio Grande Gorge became obvious, and I was intrigued by the possibility of a high raptor nesting density in the Gorge. A survey in 1979 of 20 km of the northern end of the canyon revealed a moderately high density of red-tailed hawks and prairie falcons. With the encouragement of that partial survey, and a need to assess the impact of river-running on nesting birds of prey, I made a more comprehensive survey in 1980. The results of my surveys, along with those of a 1978 helicopter survey by the BLM, are presented in this report, as well as general characterization of the area, winter use by raptors, and an assessment of factors influencing the raptor population.

  3. Introduction-Grand Challenges and small steps.

    Science.gov (United States)

    De Grandis, Giovanni; Efstathiou, Sophia

    2016-04-01

    This collection addresses two different audiences: 1) historians and philosophers of the life sciences reflecting on collaborations across disciplines, especially as regards defining and addressing Grand Challenges; 2) researchers and other stakeholders involved in cross-disciplinary collaborations aimed at tackling Grand Challenges in the life and medical sciences. The essays collected here offer ideas and resources both for the study and for the practice of goal-driven cross-disciplinary research in the life and medical sciences. We organise this introduction in three sections. The first section provides some background and context. The second motivates our take on this topic and then outlines the central ideas of each paper. The third section highlights the specificity and significance of this approach by considering: a) how this collection departs from existing literature on inter- and trans-disciplinarity, b) what is characteristic about this approach, and c) what role this suggests for the history and philosophy of the life sciences in addressing Grand Challenges. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. The Multiple Faces of Effective Grand Strategy

    Directory of Open Access Journals (Sweden)

    Bryan N. Groves

    2010-01-01

    Full Text Available Effective national leaders throughout history have deliberately developed grand strategies and successfully implemented them to attain their political goals, while also integrating and accomplishing economic, social, defense, and sometimes religious objectives. Not all leaders have been successful, however, as this process is immensely complex and can be adversely affected by the actions of other leaders around their region and the world. It bears examination, then, to determine what factors contribute to successful grand strategies and why many leaders fail to reach their stated ends. This article utilizes a historic case study approach and explores three key areas of grand strategy: universal principles, Clausewitzian approaches, and indirect approaches. I handle each separately and in distinct fashion, though some connective tissue does interlace across sections. Additionally, the unifying argument is that thoughtful, rational leaders, who weigh the costs and benefits associated with each course of action available to them, still must heed the truths embedded in these three sections to attain their objectives. Not doing so often leads to failure, unrealized goals, and a nation gone awry.

  5. Death of pastures syndrome: tissue changes in Urochloa hybrida cv. Mulato II and Urochloa brizantha cv. Marandu

    Directory of Open Access Journals (Sweden)

    N. G. Ribeiro-Júnior

    Full Text Available Abstract The quality of forage production is a prerequisite to raising livestock. Therefore, income losses in this activity, primarily cattle raising, can result in the impossibility of economic activity. Through the qualitative and quantitative anatomical study of Urochloa hybrida cv. Mulato II and U. brizantha cv. Marandu, we searched for descriptions and compared changes in the individual vegetative body from populations with death syndrome pastures (DPS. Specimens were collected at different physiological stages from farms in northern Mato Grosso. After collection, the individuals were fixed in FAA50 and stored in 70% alcohol. Histological slides were prepared from the middle third of the sections of roots, rhizomes, and leaves, and the proportions and characteristics of tissues were evaluated in healthy, intermediate, and advanced stages of DPS. Changes were compared between cultivars. With the advancement of the syndrome, the following changes were observed: a more marked decrease in the length of roots in U. hybrida; disorganization of the cortical region of the roots and rhizome cultivars; fungal hyphae in roots and aerenchyma formation in U. hybrida; a decrease in sclerenchyma fiber proportions in roots and leaves; sclerification of the epidermis of U. brizantha rhizomes; and an increase in pericyclic fibers in U. hybrida. Furthermore, there was a decrease in the volume of epidermal cells of the abaxial face of the leaves of both cultivars, with a greater reduction in U. hybrida; a gradual decrease in thickness in the midrib of leaves similar to leaf mesophyll; conduction system obstructions; partial or total cell lysis in roots and rhizomes affected by the syndrome. Obstructions in sieve tube element and companion cells, and sometimes obstruction in xylem vessel elements. The evolution of DPS in cultivars was similar, but there were variations, arising probably from the physiological response to stress, such as aerenchyma formation in

  6. Architecture, algorithm and application of OpenCV VS%OpenCV VS架构、算法及应用

    Institute of Scientific and Technical Information of China (English)

    梁华; 李兴福

    2012-01-01

    Intel OpenCV VS (video surveillance) is an efficient and universal open source platform for video object detection, tracking and trajectory analysis. However research and application are rare due to lack of material about it. The architecture and algorithm of VS platform are dissected based on analysis of OpenCV VS source code. The algorithm description and corresponding interface are presented. A high-efficiency pedestrian statistics system based on OpenCV VS architecture is provided in this paper.%Intel OpenCV VS是OpenCV内嵌的一个高效、通用的视频目标检测、跟踪和轨迹分析的开源平台,但由于没有相关资料而缺少研究和应用.在深入挖掘OpenCV VS源码的基础上,剖析了该平台的整体架构和算法体系,给出了算法描述和相应接口.在此基础上,开发了一个基于OpenCV VS架构的稳定、高效的行人统计系统.

  7. 转基因败育毛白杨雄株试管苗气孔行为观察%Observation of the Stomatal Behavior of Test-tube Plantlet of Transgenic Male Abortive Populus tomentosa Carr

    Institute of Scientific and Technical Information of China (English)

    杨金华; 朱美秋; 杜克久

    2011-01-01

    [Objective] To study the causes of abnormal stomatal behavior of test-tube plantlet. [ Method ] The change of stomatal density, stomatal size, the daily variation of stomatal aperture and stomatal open percentage of the leaves' lower epidermis of test-tube plantlets and 10-year-old plants of transgenic male abortive Populus tomentosa Carr were observed and compared in this paper by using nail polish method. [Result] The stomatal density of test-tube plantlets was less than that of the 10-year-old plant; test-tube plantlets' stomata was obviously larger than that of the 10-year-old plant; the stomatal aperture of test-tube plantlets showed wave crest, and the two peaks appeared at 9:00 and 15 :00,10-year-old plant had steady curve without evident fluctuation,and the stomatal aperture in test-tube plantlets was greater than that in the 10-year-old plant; stomatal open percentage reached 100% in test-tube plantlets during a day,a bimodal curve change was observed in stomatal open percentage of the 10-year-old plant. [Conclusion] Stomatal behavior were different between the test-tube plantlets and the 10-year-old plant of transgenic male abortive Populus tomentosa Carr.%[目的]探寻试管苗叶片气孔行为异常的原因.[方法]利用指甲油涂抹撕取法将转基因败育毛白杨雄株试管苗与10年生植株叶片下表皮制成切片,并对其气孔密度、气孔大小、气孔开度日变化、气孔开张率日变化进行观察比较.[结果]试管苗气孔密度小于10年生植株;试管苗气孔明显比10年生植株气孔大;试管苗气孔开度日变化呈明显波峰状,2个高峰分别出现在9:00和15:00,10年生植株曲线平稳,无明显波动,且试管苗气孔开度大于10年生植株;试管苗气孔开张率在一昼夜均为100%,10年生植株气孔开张率在一昼夜呈双曲线变化.[结论]转基因败育毛白杨雄株试管苗与10年生植株气孔行为存在差异.

  8. CV-Online tahab Londoni börsile / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Personaliotsinguga tegelev firma tahab tulevikus minna Londoni ja OMXi börsile. Diagramm: CV-Online Group loodab tänavu esmakordselt positiivse kulumieelse ärikasumi. Vt. samas: Üks CV-Online'i aktsionäridest on ka Google'i aktsionär; Väsinud ootamast esimest IPOt

  9. Data of evolutionary structure change: 1CV2A-2PSEA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CV2A-2PSEA 1CV2 2PSE A A ---------GA----KPFGEKKFIEIKGRRMAYIDEGTG...MPI-EWADFPEQDRDLFQAFRSQAGEELVLQDNVFVEQVLPGLILRPLSEAEMAAYREPFLAAGEARRPTLSWPRQIPIAGT-PADVVAIARDYAGWLSESP-IPKLF...tryChain> 2PSE A 2PSEA VAL CA 326 2PSE A 2PSEA...> 2PSE A 2PSEA

  10. PERUMUSAN STRATEGI BERSAING JAHE INSTAN PRODUK CV. INTRAFOOD SURAKARTA MENGGUNAKAN PERCEPTUAL MAPPING

    Directory of Open Access Journals (Sweden)

    Mohd. Harisudin

    2013-11-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui posisi bersaing jahe instan produk CV. Intrafood dan strategi bersaing yang dapat direkomendasikan untuk meraih keberasilannya. Metode dasar yang digunakan adalah analisis deskriptif. Penentuan lokasi penelitian ditentukan dengan metode purposive, yaitu CV. Intrafood Surakarta. Jenis data yang digunakan dalam penelitian ini adalah data primer dan data sekunder. Metode analisis data menggunakan analisis Perceptual Mapping. Dari hasil penelitian diperoleh informasi bahwa jahe instan produk CV Intrafood berada pada peringkat ke-2 dari produk empat jahe instan yang diperbandingkan. Atribut yang dapat dijadikan kekuatan utama dalam meningkatkan pemasaran jahe instan produk CV. Intrafood adalah manfaat produk. Sisi yang paling lemah adalah atribut desain kemasan dan kinerja produk dalam kemasan. Abstract This study aims to determine the competitive position of the product instant ginger CV. Intrafood and competitive strategy can be recommended to achieve success. The basic method used is descriptive analysis. Determining the location of the study are determined by purposive method, namely CV. Intrafood Surakarta. Data used in this study is primary data and secondary data. Method analyzed using Perceptual Mapping. From the results of the study concluded that instant ginger of CV Intrafood product ranks second of four instant ginger products are compared. Attributes that can be used as a major force in improving product marketing instant ginger CV. Intrafood is the benefits of the product. The weakest side is the packaging design and performance attributes of the product in the packaging.

  11. CV-Online tahab Londoni börsile / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Personaliotsinguga tegelev firma tahab tulevikus minna Londoni ja OMXi börsile. Diagramm: CV-Online Group loodab tänavu esmakordselt positiivse kulumieelse ärikasumi. Vt. samas: Üks CV-Online'i aktsionäridest on ka Google'i aktsionär; Väsinud ootamast esimest IPOt

  12. Detoxification of microcystin-LR in water by Portulaca oleracea cv.

    Science.gov (United States)

    Isobe, Takatoshi; Okuhata, Hiroshi; Miyasaka, Hitoshi; Jeon, Bong-Seok; Park, Ho-Dong

    2014-03-01

    Microcystin-LR (0.02 μg/ml) in the hydroculture medium of Portulaca oleracea cv., became below the detection level (microcystin estimated with protein phosphatase inhibition assay, however, remained at 37% of the initial level, indicating that microcystin-LR was transformed by P. oleracea cv. into unknown compound(s) of lower toxicity.

  13. Fast RF-CV characterization through High-Speed 1-port S-Parameter measurements

    NARCIS (Netherlands)

    Herfst, Rodolf W.; Steeneken, Peter G.; Tiggelman, Mark P.J.; Stulemeijer, Jiri; Schmitz, Jurriaan

    2012-01-01

    We present a fast radio frequency–capacitancevoltage (RF-CV) method to measure the CV relation of an electronic device. The approach is more accurate, much faster, and more cost effective compared to the existing off-the-shelf solutions. Capacitances are determined using a single-frequency 1-port S-

  14. Effect of 6-BA on nodal explant bud sproutings of Coffea arabica cv. Mundo Novo Efeito de 6-BA na brotação de gemas de explantes nodais de Coffea arabica cv. Mundo Novo

    Directory of Open Access Journals (Sweden)

    Luis Carlos da Silva Ramos

    2005-01-01

    Full Text Available Coffee plants can be micropropagated by nodal bud sprouting using the 6-benzylaminopurine (6-BA hormone. However, literature reports the use of a wide range of 6-BA, from 0.5 to 88.8 µM L-1. So, this study was performed to narrow that range. Nodal explants of Coffea arabica cv Mundo Novo obtained from in vitro plantlets were inoculated on gelled-MS medium supplemented with different concentrations of 6-BA. Two assays were carried out: in the first one, 6-BA was used at concentrations of 0, 5, 25, 50, and 100 µM L-1, being evaluated at 43 and 123 days. In the second experiment, dosis of 10, 20 and 30 µM L-1, have evaluated at 65 and 100 days. Treatments with 6-BA induced multiple sprouting from the nodal explants, which were best characterized around 100 days after inoculation. The nodal explants grew taller and showed multiple shoots, whereas the effect of 6-BA at 5 to 25 µM L-1 was similar to that with higher concentrations (50 and 100 µM L-1. Nodal explants yielded from 2.9 to 6.0 buds per node, achieving height of 1.3 to 1.5 cm at 5 to 25 µM L-1 of 6-BA, whereas they yielded from 4.3 to 4.9 buds per node but the sprouting grew about 0.8 cm at 50 and 100 µM L-1 of 6-BA. This study indicated that multiple sprouting of lateral buds can be induced by lower concentrations of 6-BA, for example, from 10 to 30 µM L-1, diminishing possible risks of somaclonal variation due to high levels of hormone concentration.O cafeeiro pode ser micropropagado via brotação de gemas laterais, aplicando o regulador de crescimento 6-benzilaminopurina (6-BA. Entretanto, a literatura apresenta ampla variação da dose empregada, desde 0.5 a 88.8 µM L-1. Assim, este estudo visou otimizar doses para explantes nodais do cafeeiro C. arabica cv Mundo Novo. Explantes nodais, obtidos de plântulas cultivadas in vitro, foram inoculados em meio MS geleificado, com adição de diferentes concentrações de 6-BA. Foram feitos dois experimentos: no primeiro, 6-BA foi

  15. CV characteristics of polycrystalline sige films with low GE concentration

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, Ricardo Cotrin [School of Electrical and Computer Engineering (FEEC) and Center for Semiconductor Components(CCS), State University of Campinas - UNICAMP, Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil); Doi, Ioshiaki [School of Electrical and Computer Engineering (FEEC) and Center for Semiconductor Components(CCS), State University of Campinas - UNICAMP, Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil)]. E-mail: doi@led.unicamp.br; Diniz, Jose Alexandre [School of Electrical and Computer Engineering (FEEC) and Center for Semiconductor Components(CCS), State University of Campinas - UNICAMP, Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil); Swart, Jacobus Willibrordus [School of Electrical and Computer Engineering (FEEC) and Center for Semiconductor Components(CCS), State University of Campinas - UNICAMP, Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil); Pinto Zakia, Maria Beny [Center for Semiconductor Components (CCS), State University of Campinas (UNICAMP), Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil)

    2006-12-15

    SiGe alloys are currently used for HBT and MOS as epitaxial layers for base or strained channel, respectively. In the poly phase, SiGe has been studied as a replacement for poly-Si in MOS gates due to its lower thermal budget and gate depletion and also due to the Workfunction Engineering for V {sub t} adjustments. However, for application to CMOS technology as poly-SiGe gates, others constrains emerge such as quality of the oxide interface and etch chemistry. For both applications, the Ge fraction normally lies between 20% and 40%. In this study, authors use a low Ge contents (1%) poly-SiGe thin films aiming for MOS gate electrode. The Ge fraction was determined by RBS analysis. 230 nm thick samples were deposited onto 10 nm thermally oxidized <1 0 0>, p-type Si substrates using silane and germane. Films were deposited in the temperature of 500 deg. C and total pressure of 667 Pa (5 Torr) by vertical LPCVD. The samples were doped using {sup 31}P{sup +} ion implantation from 5 x 10{sup 14} cm{sup -2} up to 2 x 10{sup 16} cm{sup -2} and annealed by RTP (40 s) from 500 deg. C up to 900 deg. C. R {sub s} values were obtained by 4-point probe technique and CV curves were extracted from nMOS capacitors with 200 {mu}m diameter. The same processing steps were used to fabricate similar poly-Si samples and capacitors for comparison. The poly-SiGe samples presented R {sub s} values one order of magnitude lower than poly-Si and CV analysis of nMOS capacitors showed very good characteristics. The 1% Ge in the alloy ensures a low thermal budget for the overall process. Although a relatively high annealing temperature (800 deg. C) must be used to reduce oxide charge and interface traps, the temperature is well below the necessary for poly-Si processing and can allow formation of the shallow junctions needed for next technological nodes.

  16. Rat and poultry feeding studies with soybean meal produced from imidazolinone-tolerant (CV127) soybeans.

    Science.gov (United States)

    He, Xiaoyun; de Brum, Paulo A R; Chukwudebe, Amechi; Privalle, Laura; Reed, Andrew; Wang, Yanqing; Zhou, Cui; Wang, Cuiyan; Lu, Jing; Huang, Kunlun; Contri, Daniela; Nakatani, Andreia; de Avila, Valdir S; Klein, Claudete H; de Lima, Gustavo J M M; Lipscomb, Elizabeth A

    2016-02-01

    The safety and nutritional properties of CV127 soybeans were evaluated in rat and broiler feeding studies. Some episodic differences were observed between rats fed CV127, Conquista, and the standard diet for the endpoints examined. None of these differences were considered treatment related, adverse, or biologically meaningful. In general, birds fed diets containing CV127, Conquista, or Monsoy 8001 showed no significant differences in growth and performance response variables. Chickens fed diets containing Coodetec 217 had lower body weight and weight gain for all developmental periods compared to CV127, but no significant differences were found in feed conversion for the two diets during any development period. The results of both feeding studies demonstrate that CV127 soybeans are as safe, wholesome, and nutritionally valuable as the other soybean meals tested, including those varieties for which histories of safe use have been established and well documented.

  17. Needs assessment for the Greenway Grand Forks-East Grand Forks development and public education

    Science.gov (United States)

    Munski, Laura

    Following the flood of 1997, the U.S. Army Corps of Engineers included the Greenway Grand Forks---East Grand Forks (the Greenway) as a flood control measure for Grand Forks, North Dakota and East Grand Forks, Minnesota. It extends along both the Red River of the North and the Red Lake River, encompassing 2200 acres of land. The cities of Grand Forks and East Grand Forks hired consultants to assist with the postflood planning process. The planning process culminated with the Red River of the North Greenway Final Report (Flink, 1998). The purpose of this study was to determine if the development of the Greenway addressed the objectives of the planning report. The history of the land adjacent to the rivers was reviewed to document the progression of riverfront development. Anecdotal evidence was collected, field observations were made, city council minutes were reviewed, Greenway Technical Committee members were interviewed, Greenway Technical Committee minutes were reviewed, and the Greenway Grand Forks---East Grand Forks survey results were reviewed to determine if the objectives of the Red River of the North Greenway Final Report were addressed. A cross section survey was designed by Laura Munski for this dissertation research. The survey was approved by the Greenway Technical Committee. The survey collected both quantitative and qualitative data from the community. The purpose of the survey portion of the research project was to ascertain how residents were kept informed of activities on the Greenway and what amenities residents were using on the Greenway and to solicit their comments regarding the Greenway. The results of the survey research were used in both marketing and event planning for the Greenway. The singular qualitative survey question gave respondents an opportunity to share their comments regarding the Greenway. The qualitative data analysis provided insight to the amenities and educational programs desired by respondents, their concerns regarding the

  18. MICROPROPAGAÇÃO DO KIWI CV. HAYWARD

    Directory of Open Access Journals (Sweden)

    SCALIZE FÁBIO EDUARDO

    2001-01-01

    Full Text Available O presente trabalho teve como objetivo desenvolver um protocolo para a obtenção de mudas de kiwi (Actinidia chinensis Planch, cv. Hayward, por meio do cultivo in vitro de cotilédones. Utilizou-se o meio de MURASHIGE & SKOOG (1962 -- MS, suplementado com dois tipos de auxina (AIA e AIB e uma citocinina (BAP. Foram verificados os efeitos de três doses de auxinas (0,125; 0,250 e 0,375 mg.L-1, combinadas com três doses de citocinina (0,5; 1,0 e 1,5 mg.L-1 na capacidade morfogênica dos explantes. Procedeu-se o estudo histológico dos órgãos das plântulas obtidas in vitro, e verificou-se, também, a capacidade de aclimatação das mudas ex vitro. A menor dose de AIB (0,125 mg.L-1, independentemente das doses de BAP, foi a mais eficaz na morfogênese dos explantes. Não foram verificadas alterações histológicas e anatômicas das plântulas obtidas in vitro. Aos três meses após o cultivo ex vitro dos explantes, verificou-se a sobrevivência de 88% das plantas transplantadas em condições de campo.

  19. Ultrastructure and germination of Vitis vinifera cv. Loureiro pollen.

    Science.gov (United States)

    Abreu, I; Costa, I; Oliveira, M; Cunha, M; de Castro, R

    2006-08-01

    The cultivar Loureiro of Vitis vinifera is one of the most economically important, recommended in almost the totality of the Região Demarcada dos Vinhos Verdes. In vineyards, the grape productivity of this cultivar is normal while in others it is extremely low. The aim of this work was to study the morphology and germination of Vitis vinifera cv. Loureiro pollen with high and low productivity. The pollen grain was examined under light, transmission and scanning electron microscopy. Typically V. vinifera pollen present three furrows but in the cultivar Loureiro we found tricolporated and acolporated (without furrows or pores) pollen grains. Both pollen types present generative and vegetative cells with the usual aspect and a dense cytoplasm rich in organelles. In the acolporated pollen a continuous exine layer and an irregular intine layer were observed. Differences were found in the starch accumulation, since only in tricolporated pollen abundant plastids filled with numerous starch granules were observed. To determine the causes of the low productivity of this cultivar we tested pollen viability by the fluorochromatic reaction and pollen germinability by in vitro assays. We observed that the acolporated pollen grain is viable, but no germination was recorded.

  20. EVALUASI SISTEM INFORMASI PERSEDIAAN PADA CV. SARANA TELEMAXINDO

    Directory of Open Access Journals (Sweden)

    Nelly Nelly

    2010-10-01

    Full Text Available Evaluation objectives of inventory information systems at CV. Facilities Telemaxindo was to evaluate the weaknesses of the implementation of internal control, as well as estimating and assessing possible risks that might arise. The research method used is literature study of reference books in the library, and field study consisted of observation, interviews with relevant parties, creating check lists, check the inventory information systems documentation, and testing of inventory application systems. The results are findings of control weaknesses in the implementation of security management, operational management control, boundary control, input control, and output control on the present inventory information system. These weaknesses could pose a risk and if not dealt soon it could hurt the company. The resulting conclusion of the security management control, boundary control, control input, and output control is not running well so that it needs to be improved in accordance with the recommendations, while operational management control has been running fairly well.Keywords: evaluation, information system, inventory

  1. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean

    Institute of Scientific and Technical Information of China (English)

    Yau Sang Chan; Jack Ho Wong; Evandro Fei Fang; Wen Liang Pan; Tzi Bun Ng

    2012-01-01

    A 5.4-kDa antifungal peptide,with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC5o=3 μM),Setospaeria turcica and Bipolaris maydis,was isolated from the seeds of Phaseolus vulgaris cv.brown kidney bean.The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75.The antifungal activity of the peptide against M.arachidicola was stable in the pH range 3-12 and in the temperature range 0℃ to 80℃.There was a slight reduction of the antifungal activity at pH 2 and 13,and the activity was indiscernible at pH 0,1,and 14.The activity at 90℃ and 100℃ was slightly diminished.Deposition of Congo red at the hyphal tips of M.arachidicola was induced by the peptide indicating inhibition of hyphal growth.The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells,in contrast to the presence of such activity of other antifungal peptides,indicates that different domains are responsible for the antifungal and antiproliferative activities.

  2. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean.

    Science.gov (United States)

    Chan, Yau Sang; Wong, Jack Ho; Fang, Evandro Fei; Pan, Wen Liang; Ng, Tzi Bun

    2012-04-01

    A 5.4-kDa antifungal peptide, with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC(50)= 3 μM), Setospaeria turcica and Bipolaris maydis, was isolated from the seeds of Phaseolus vulgaris cv. brown kidney bean. The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75. The antifungal activity of the peptide against M. arachidicola was stable in the pH range 3-12 and in the temperature range 0°C to 80°C. There was a slight reduction of the antifungal activity at pH 2 and 13, and the activity was indiscernible at pH 0, 1, and 14. The activity at 90°C and 100°C was slightly diminished. Deposition of Congo red at the hyphal tips of M. arachidicola was induced by the peptide indicating inhibition of hyphal growth. The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells, in contrast to the presence of such activity of other antifungal peptides, indicates that different domains are responsible for the antifungal and antiproliferative activities.

  3. Absolute properties of the spotted eclipsing binary star CV Bootis

    CERN Document Server

    Torres, Guillermo; Lacy, Claud H Sandberg

    2008-01-01

    We present new V-band differential brightness measurements as well as new radial-velocity measurements of the detached, circular, 0.84-day period, double-lined eclipsing binary system CV Boo. These data along with other observations from the literature are combined to derive improved absolute dimensions of the stars for the purpose of testing various aspects of theoretical modeling. Despite complications from intrinsic variability we detect in the system, and despite the rapid rotation of the components, we are able to determine the absolute masses and radii to better than 1.3% and 2%, respectively. We obtain M(A) = 1.032 +/- 0.013 M(Sun) and R(B) = 1.262 +/- 0.023 R(Sun) for the hotter, larger, and more massive primary (star A), and M(B) = 0.968 +/- 0.012 M(Sun) and R(B) = 1.173 +/- 0.023 R(Sun) for the secondary. The estimated effective temperatures are 5760 +/- 150 K and 5670 +/- 150 K. The intrinsic variability with a period about 1% shorter than the orbital period is interpreted as being due to modulatio...

  4. Future Objectives of Maintenance Management in the CV Group

    CERN Document Server

    Annila, L

    1999-01-01

    The ST/CV group has an extensive maintenance contract with a multinational consortium (Gematec-F/I/DE). Annually, the contract costs the group about 6 MCHF. Today the technical monitoring of the performance within the contract has become too irregular and different people's roles have not been sufficiently well defined. It has therefore been decided that all the technical, financial and performance-indicating data should be centrally co-ordinated within the group. The objective is to enable an efficient follow-up of the contract with a view to optimization. Another objective is to migrate to the new, more proficient version of the Computer Aided Maintenance Management (CAMM) software and learn to use it to its full potential. This means using its statistics and analysis features in addition to controlling the maintenance activities, spare-parts store, costs, etc. A further aim is to develop standards for the maintenance and the CAMM. This paper will discuss these objectives and the schedule for their implemen...

  5. Seven health physics calculator programs for the HP-41CV

    Energy Technology Data Exchange (ETDEWEB)

    Rittmann, P.D.

    1984-08-01

    Several user-oriented programs for the Hewlett-Packard HP-41CV are explained. The first program builds, stores, alters, and ages a list of radionuclides. This program only handles single- and double-decay chains. The second program performs convenient conversions for the six nuclides of concern in plutonium handling. The conversions are between mass, activity, and weight percents of the isotopes. The source can be aged and/or neutron generation rates can be computed. The third program is a timekeeping program that improves the process of manually estimating and tracking personnel exposure during high dose rate tasks by replacing the pencil, paper, and stopwatch method. This program requires a time module. The remaining four programs deal with computations of time-integrated air concentrations at various distances from an airborne release. Building wake effects, source depletion by ground deposition, and sector averaging can all be included in the final printout of the X/Q - Hanford and X/Q - Pasquill programs. The shorter versions of these, H/Q and P/Q, compute centerline or sector-averaged values and include a subroutine to facilitate dose estimation by entering dose factors and quantities released. The horizontal and vertical dispersion parameters in the Pasquill-Gifford programs were modeled with simple, two-parameter functions that agreed very well with the usual textbook graphs. 8 references, 7 appendices.

  6. Infrared small target detection technology based on OpenCV

    Science.gov (United States)

    Liu, Lei; Huang, Zhijian

    2013-09-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  7. A virtual computing infrastructure for TS-CV SCADA systems

    CERN Document Server

    Poulsen, S

    2008-01-01

    In modern data centres, it is an emerging trend to operate and manage computers as software components or logical resources and not as physical machines. This technique is known as â€ワvirtualisation” and the new computers are referred to as â€ワvirtual machines” (VMs). Multiple VMs can be consolidated on a single hardware platform and managed in ways that are not possible with physical machines. However, this is not yet widely practiced for control system deployment. In TS-CV, a collection of VMs or a â€ワvirtual infrastructure” is installed since 2005 for SCADA systems, PLC program development, and alarm transmission. This makes it possible to consolidate distributed, heterogeneous operating systems and applications on a limited number of standardised high-performance servers in the Central Control Room (CCR). More generally, virtualisation assists in offering continuous computing services for controls and maintaining performance and assuring quality. Implementing our systems in a vi...

  8. Antidiabetic and anticancer activities of Mangifera indica cv. Okrong leaves

    Science.gov (United States)

    Ganogpichayagrai, Aunyachulee; Palanuvej, Chanida; Ruangrungsi, Nijsiri

    2017-01-01

    Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract of Mangifera indica cv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast α-glucosidase and rat intestinal α-glucosidase were determined using 1 mM of p-nitro phenyl-α-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic α-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-α-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast α-glucosidase with the IC50 of 0.0503 and 0.5813 mg/ml, respectively, against rat α-glucosidase with the IC50 of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC50 of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at ≥200 μg/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potential in vitro. PMID:28217550

  9. Antidiabetic and anticancer activities of Mangifera indica cv. Okrong leaves.

    Science.gov (United States)

    Ganogpichayagrai, Aunyachulee; Palanuvej, Chanida; Ruangrungsi, Nijsiri

    2017-01-01

    Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract of Mangifera indica cv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast α-glucosidase and rat intestinal α-glucosidase were determined using 1 mM of p-nitro phenyl-α-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic α-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-α-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast α-glucosidase with the IC50 of 0.0503 and 0.5813 mg/ml, respectively, against rat α-glucosidase with the IC50 of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC50 of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at ≥200 μg/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potential in vitro.

  10. Antidiabetic and anticancer activities of Mangifera indica cv. Okrong leaves

    Directory of Open Access Journals (Sweden)

    Aunyachulee Ganogpichayagrai

    2017-01-01

    Full Text Available Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract of Mangifera indica cv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast α-glucosidase and rat intestinal α-glucosidase were determined using 1 mM of p-nitrophenyl-α-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic α-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-α-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast α-glucosidase with the IC 50 of 0.0503 and 0.5813 mg/ml, respectively, against rat α-glucosidase with the IC 50 of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC 50 of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at ≥200 μg/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potential in vitro.

  11. The early-type close binary CV Velorum revisited

    CERN Document Server

    Yakut, K; Morel, T; Morel, Th.

    2007-01-01

    Our goal was to improve the fundamental parameters of the massive close double-lined eclipsing B2.5V+B2.5V binary CV Velorum.We gathered new high-resolution echelle spectroscopy on 13 almost consecutive nights covering essentially two orbits. We computed a simultaneous solution to all the available high-quality radial-velocity and light data with the latest version of the Wilson-Deviney code. We obtained the following values for the physical parameters: $M_1 = 6.066(74) M_\\odot$, $M_2 = 5.972(70) M_\\odot$, $R_1 = 4.126(24) R_\\odot$, $R_2 = 3.908(27) R_\\odot$, $\\log L_1 = 3.20(5) L_\\odot$, and $\\log L_2 = 3.14(5) L_\\odot$. The quoted errors contain a realistic estimate of systematic uncertainties mainly stemming from the effective temperature estimation. We derived abundances for both components and found them to be compatible with those of B stars in the solar neighbourhood. We discovered low-amplitude periodic line-profile variations with the orbital frequency for both components. Their interpretation requir...

  12. [Establishment of embryogenic cell suspension culture and plant regeneration of edible banana Musa acuminata cv. Mas (AA)].

    Science.gov (United States)

    Wei, Yue-Rong; Huang, Xue-Lin; Li, Jia; Huang, Xia; Li, Zhe; Li, Xiao-Ju

    2005-01-01

    Conventional breeding for dual resistance of disease and pest of Musa cultivars remains a difficult endeavor, as the plant is polyploidic and high in sterility. Biotechnological techniques, eg., genetic engineering, in vitro mutation breeding, or protoplast fusion, may overcome the difficulties and improve the germplasm. Establishment of a stable embryogenic cell suspension (ECS) is a prerequisite for any of the biotechnological breeding methods. In this study an embryogenic cell suspension was established from immature male flower of Musa acuminata cv. Mas (AA), a popular commercial variety of banana in the South-East Asian region. After culture for 5-6 months on callus induction media, which consisted of MS salts, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 4.1 micromol/L biotin, 5.7 micromol/L indoleacetic acid (IAA), 5.4 micromol/L naphthaleneacetic acid (NAA), other vitamins, 87 mmol/L sucrose, and solidified with 7 g/L agarose, meristematic globules and yellow, friable embryogenic cultures were induced from the explants of 1-15th row young floral hands of immature male flowers. Of the four treatments of 2,4-D, 9 micromol/L was the most effective on the callus induction, it transformed 40.96% and 7.45% of the cultivated male floral hands into callus and embryogenic callus respectively. The explants to produce highest frequency of the embryogenic calli were floral hands of 6 to 12th rows, which generated 5.79% of the embryogenic calli. Suspension cultures were initiated from these embryogenic calli in liquid medium supplemented with 4.5 micromol/L 2, 4-D. After sieving selection of the cultures using a stainless steel metallic strainer with pore sizes of 154 microm at 15 day intervals for 3 months, homogeneous and yellow embryogenic cell suspensions, composed of single cells and small cell aggregates, were established. Based upon the growth quantity and growth rate of ECS, it was determined that the appropriate inoculum was 2.0 mL PCV

  13. STRATEGI PENGEMBANGAN USAHA SAPI POTONG (STUDI KASUS CV MITRA TANI FARM

    Directory of Open Access Journals (Sweden)

    Shally Alpriany Aisyah

    2014-09-01

    Full Text Available ABSTRACTThe purpose of this study were to 1 analyze the internal and external environmental conditions of CV Mitra Tani Farm, 2 identify and define strategies that affect the cattle breeding business development at CV Mitra Tani Farm, and 3 set the strategic priorities in the development of cattle breeding business in CV Mitra Tani Farm. Analysis strategies model (David, 2009 used were the analysis of the internal and external environment, strategy formulation and strategic priorities. At the stage of strategy formulation, SWOT 4 Quadrants were used. The results showed that the difference between the weighted scores of opportunities and threats as well as strengths and weaknesses is located in first quadrant of the SWOT 4 quadrant matrix. The first quadrant (growth quadrant has eight strategies, but only three can be applied appropriately. The results of the value of interest using quantitative strategic planning matrix strategy used is the market expansion strategy, then the strategy of product development, and concentric diversification strategy. Therefore, in carrying out market expansion strategy, CV Mitra Tani Farm needs to do breakthrough campaign. In product development strategy, preferably CV Mitra Tani Farm makes processed beef products which are unique and still rare in the market today.Keywords: strategy, business beef cattle, CV Mitra Tani Farm, SWOT 4K, QSPMABSTRAKTujuan penelitian ini yaitu 1 menganalisis kondisi lingkungan internal dan eksternal CV Mitra Tani Farm, 2 mengidentifikasi dan menetapkan strategi yang memengaruhi dalam pengembangan bisnis sapi potong di CV Mitra Tani Farm, dan 3 menetapkan prioritas strategi dalam pengembangan usaha peternakan sapi potong di CV Mitra Tani Farm. Model analisis strategi (David, 2009 yang digunakan yaitu analisis lingkungan internal dan eksternal, perumusan strategi dan prioritas strategi. Pada tahap perumusan strategi digunakan SWOT 4 Kuadran. Hasil penelitian menunjukkan bahwa selisih

  14. The periodicity of Grand Solar Minimum

    Science.gov (United States)

    Velasco Herrera, Victor Manuel

    2016-07-01

    The sunspot number is the most used index to quantify the solar activity. Nevertheless, the sunspot is a syn- thetic index and not a physical index. Therefore, we should be careful to use the sunspot number to quantify the low (high) solar activity. One of the major problems of using sunspot to quantify solar activity is that its minimum value is zero. This zero value hinders the reconstruction of the solar cycle during the Maunder minimum. All solar indexes can be used as analog signals, which can be easily converted into digital signals. In con- trast, the conversion of a digital signal into an analog signal is not in general a simple task. The sunspot number during the Maunder minimum can be studied as a digital signal of the solar activity In 1894, Maunder published a discovery that has maintained the Solar Physics in an impasse. In his fa- mous work on "A Prolonged Sunspot Minimum" Maunder wrote: "The sequence of maximum and minimum has, in fact, been unfailing during the present century [..] and yet there [..], the ordinary solar cycle was once interrupted, and one long period of almost unbroken quiescence prevailed". The search of new historical Grand solar minima has been one of the most important questions in Solar Physics. However, the possibility of estimating a new Grand solar minimum is even more valuable. Since solar activity is the result of electromagnetic processes; we propose to employ the power to quantify solar activity: this is a fundamental physics concept in electrodynamics. Total Solar Irradiance is the primary energy source of the Earth's climate system and therefore its variations can contribute to natural climate change. In this work, we propose to consider the fluctuations in the power of the Total Solar Irradiance as a physical measure of the energy released by the solar dynamo, which contributes to understanding the nature of "profound solar magnetic field in calm". Using a new reconstruction of the Total Solar Irradiance we found the

  15. Westernmost Grand Canyon incision: Testing thermochronometric resolution

    Science.gov (United States)

    Fox, M.; Tripathy-Lang, A.; Shuster, D. L.; Winn, C.; Karlstrom, K.; Kelley, S.

    2017-09-01

    The timing of carving of Grand Canyon has been debated for over 100 years with competing endmember hypotheses advocating for either a 70 Ma (;old;) or history and corresponding estimates of landscape evolution have been in debate. In particular, 4He/3He thermochronometric data record the distribution of radiogenic 4He (from the 238U, 235U and 232Th decay series) within an individual apatite crystal and thus are highly sensitive to the thermal history corresponding to landscape evolution. However, there are several complicating factors that make interpreting such data challenging in geologic scenarios involving reheating. Here, we analyze new data that provide measures of the cooling of basement rocks at the base of westernmost Grand Canyon, and use these data as a testbed for exploring the resolving power and limitations of 4He/3He data in general. We explore a range of thermal histories and find that these data are most consistent with a ;young; Grand Canyon. A problem with the recovered thermal history, however, is that burial temperatures are under predicted based on sedimentological evidence. A solution to this problem is to increase the resistance of alpha recoil damage to annealing, thus modifying He diffusion kinetics, allowing for higher temperatures throughout the thermal history. This limitation in quantifying radiation damage (and hence crystal retentivity) introduces non-uniqueness to interpreting time-temperature paths in rocks that resided in the apatite helium partial retention zone for long durations. Another source of non-uniqueness, is due to unknown U and Th distributions within crystals. We show that for highly zoned with a decrease in effective U of 20 ppm over the outer 80% of the radius of the crystal, the 4He/3He data could be consistent with an ;old; canyon model. To reduce this non-uniqueness, we obtain U and Th zonation information for separate crystals from the same rock sample through LA-ICP-MS analysis. The observed U and Th

  16. La "misteriosa" scrittura grande: paleografia e storia

    OpenAIRE

    Modesti, Maddalena; Iannacci, Lorenza; Zuffrano, Annafelicia

    2010-01-01

    Nel 1952 Jan Olof Tjäder riusciva finalmente a decifrare la cosiddetta misteriosa scrittura grande che caratterizza la prima riga di quattro papiri ravennati contenenti protocolli dei gesta municipalia (504-625 d. C.) e a individuarne la base grafica nella corsiva romana antica. Questo permetteva quindi al Tjäder di dimostrare che l’antica scrittura comune romana non era sopravvissuta, dopo il IV secolo, solo nelle litterae caelestes riservate ai documenti imperiali (cfr. Th.9.19.3, 367 d. C....

  17. Inflationary Reheating in Grand Unified Theories

    CERN Document Server

    Bassett, B A; Bassett, Bruce A.; Tamburini, Fabrizio

    1998-01-01

    Grand unified theories may display multiply interacting fields with strong coupling dynamics. This poses two new problems: (1) What is the nature of chaotic reheating after inflation, and (2) How is reheating sensitive to the mass spectrum of these theories ? We answer these questions in two interesting limiting cases and demonstrate an increased efficiency of reheating which strongly enhances non-thermal topological defect formation, including monopoles and domain walls. Nevertheless, the large fluctuations may resolve this monopole problem via a modified Dvali-Liu-Vachaspati mechanism in which non-thermal destabilsation of discrete symmetries occurs at reheating.

  18. Produccion de plantas grandes usando minicontenedores

    Science.gov (United States)

    R. Kasten Dumroese; Thomas D. Landis

    2012-01-01

    En América del Norte hay cada vez más interés por la producción híbrida o mixta. La misma consiste en cultivar plantines en contenedores de pequeño volumen y luego trasplantarlos; el trasplante se puede realizar a canteros en el suelo como en la producción a raíz desnuda o bien a contenedores más grandes. Originalmente se llamaban plantines "plug+", "...

  19. Grand Banquet of CDM for Power Enterprises

    Institute of Scientific and Technical Information of China (English)

    Peng Yuanchang; Jin Wen

    2007-01-01

    @@ The Kyoto Protocol was taken into effect on Feb. 16, 2005. It requires developed countries to reduce their greenhouse gas emissions, but it doesn't set binding limits on developing countries, such as China. The developed countries found that it is more cost-effective to reduce the emissions in developing countries than in their own. Therefore, the CDM emerged as the times require. Due to unfamiliarity and complicatedness, Chinese enterprises had been hesitating and taking wait-and-see attitude toward CDM, but they couldn't resist the attraction of free dinner of CDM, more and more enterprises started to attend the grand banquet of CDM since 2006.

  20. FOCAL Grande Utopia EM音箱

    Institute of Scientific and Technical Information of China (English)

    成师

    2008-01-01

    对于一个真正在音响领域有所追求的品牌来讲,创新与改进是没有尽头的。尽管愈往前发展,技术的突破度会越来越小,但这仍不妨碍品牌向着高境界的持续前行。近期,法国劲浪(FOCAL)最新发表的Grande Utopia EM音箱,便是展示其研发成果的生动例证。

  1. Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets

    Directory of Open Access Journals (Sweden)

    Couée Ivan

    2009-03-01

    Full Text Available Abstract Background Besides being essential for plant structure and metabolism, soluble carbohydrates play important roles in stress responses. Sucrose has been shown to confer to Arabidopsis seedlings a high level of tolerance to the herbicide atrazine, which causes reactive oxygen species (ROS production and oxidative stress. The effects of atrazine and of exogenous sucrose on ROS patterns and ROS-scavenging systems were studied. Simultaneous analysis of ROS contents, expression of ROS-related genes and activities of ROS-scavenging enzymes gave an integrative view of physiological state and detoxifying potential under conditions of sensitivity or tolerance. Results Toxicity of atrazine could be related to inefficient activation of singlet oxygen (1O2 quenching pathways leading to 1O2 accumulation. Atrazine treatment also increased hydrogen peroxide (H2O2 content, while reducing gene expressions and enzymatic activities related to two major H2O2-detoxification pathways. Conversely, sucrose-protected plantlets in the presence of atrazine exhibited efficient 1O2 quenching, low 1O2 accumulation and active H2O2-detoxifying systems. Conclusion In conclusion, sucrose protection was in part due to activation of specific ROS scavenging systems with consequent reduction of oxidative damages. Importance of ROS combination and potential interferences of sucrose, xenobiotic and ROS signalling pathways are discussed.

  2. 碱蓬下胚轴试管再生植株的研究%STUDIES ON HYPOCOTYL CULTURE IN VITRO AND PLANTLET REGENERATION OF SUAEDA GLAUCA

    Institute of Scientific and Technical Information of China (English)

    王兴安; 马宗琪; 侯元同; 张举仁; 张慧; 赵彦修

    2001-01-01

    Hypocotyl of commo n seepweed [Suaeda glauca (Bunge) Bunge]seedling we re cultured on four MS agar media of different concentrations NAA、2, 4-D、6-BA and IAA. The calli were induced on the media. The induction frequency of calli a mounted to 94.64%~100.00%.The calli were transferred to medium that contained h igher concentration 6-BA. The induction frequency of adventitious buds were from 25.25% to 60%. Adventitious buds were transferred to the MS medium that conta ined higher concentrations IBA. After ten days, the calli grew roots and got plantlet regeneration in vitro.%碱蓬[Suaedaglanca(Bunge)Bunge]幼苗的下胚轴接种在MS加不同浓度NAA、2,4-D、6-BA和IAA的培养基上,愈伤组织诱导频率为94.64%~100%.愈伤组织转移到以6-BA为主的分化培养基上,不定芽分化频率为25.25%~60%.不定芽转入IBA生根培养基后,10d左右生根,得到再生植株.

  3. Comparative analysis of bioactive N-alkylamides produced by tissue culture raised versus field plantlets of Spilanthes ciliata using LC-Q-TOF (HRMS).

    Science.gov (United States)

    Bhat, Zubair Shanib; Jaladi, Neeharika; Khajuria, Ravi Kant; Shah, Zeeshan Hamid; Arumugam, Neelakantan

    2016-04-01

    Spilanthes ciliata (S. ciliata) is a perennial herb of global importance owing to its luscious source of bioactive fatty acid derived amides known as N-alkylamides. It finds application in skin creams, mouth gels and toothpastes. Despite multifaceted applications, a major limitation associated for its commercial application is the scarcity of contamination free plant source, fluctuations in active metabolites due to variation in extraction procedures, and lack of rapid qualitative method for alkylamide profiling. In the current work, attempts were made to 1) optimize conditions for mass propagation of contamination free plants of S. ciliata by tissue culture using leaf discs as explants, 2) establish an optimum extraction ratio of plant/solvent (w/v) for maximum elution of alkylamides and 3) develop a rapid method for qualitative estimation of alkylamide from in vitro raised plants in comparison with that of the field grown counterpart by using LC-Q-TOF (HRMS). To the best of our knowledge, this is the first qualitative report on alkylamide profile of micropropagated whole plant of Spilanthes. The correlation pattern reported in this study may form the basis for using tissue culture raised plantlets of S. ciliata as potential source of bioactive alkylamides on industrial scale.

  4. FLORAL RECEPTACLE CULTURE IN VITRO AND PLANTLET REGENERATION IN GERBERA JAMESONII BOLUS%非洲菊花托培养和植株再生

    Institute of Scientific and Technical Information of China (English)

    郑秀芳; 李名扬

    2001-01-01

    The floral receptacles of 4 cultivars of Gerbera jamesonii Bolux were used as explants for rapid micropropagation. A satisfactory procedure was established with a multiplication rate of 5 times/20d~7d. The optimum medium was B5+10mg/L BA+0.1 mg/L IAA for sahoot induction,MS+1 mg/L BA+0.1 mg/L IAA for subculture and 1/2MS+0.01mg/L IAA for rooting. The regenerated plantlete proved to be genetically stable with no abnormality when examined cytogically and morphologically.%建立了以非洲菊幼小花托为外植体的离体快繁技术,繁殖系数可达5倍/20天~7倍/20天左右。诱导培养基以B5+l0 mg/L BA+0.1 mg/L IAA最佳,继代增殖以MS+1 mg/L BA+0.1mg/L LAA为宜,生根培养以1/2MS+0.01mg/L IBA效果最好。细胞学和形态学观察表明再生植株的形态和染色体无异常现象,遗传性稳定好。

  5. Alternation of light/dark period priming enhances clomazone tolerance by increasing the levels of ascorbate and phenolic compounds and ROS detoxification in tobacco (Nicotiana tabacum L.) plantlets.

    Science.gov (United States)

    Darwish, Majd; Lopez-Lauri, Félicie; Vidal, Véronique; El Maâtaoui, Mohamed; Sallanon, Huguette

    2015-07-01

    The effect of the alternation of light/dark periods (AL) (16/8 min light/dark cycles and a photosynthetic photon flux density (PPFD) of 50 μmol photons m(-2) s(-1) for three days) to clarify the mechanisms involved in the clomazone tolerance of tobacco plantlets primed with AL was studied. Clomazone decreased PSII activity, the net photosynthetic rate (Pn), and the ascorbate and total polyphenol contents and increased H2O2 and starch grain accumulation and the number of the cells that underwent programmed cell death (PCD). The pretreatment with AL reduced the inhibitory effect of clomazone on the PSII activity and photosynthesis, as indicated by the decreases in the H2O2 and starch grain accumulation and the PCD levels, and increased the content of ascorbate and certain phenolic compounds, such as chlorogenic acid, neochlorogenic acid and rutin. The AL treatment could promote photorespiration via post-illumination burst (PIB) effects. This alternative photorespiratory electron pathway may reduce H2O2 generation via the consumption of photochemical energy, such as NADH+H(+). At 10 days (D10) of AL treatment, this process induced moderate stress which stimulates H2O2 detoxification systems by increasing the activity of antioxidant enzymes and the biosynthesis of antioxidant components. Therefore, the PCD levels provoked by clomazone were noticeably decreased.

  6. Effect of applied synthetic auxin on root growth in plantlet propagation by cuttage and tissue culture; Sashiki to soshiki baiyo ni okeru gosei auxin rui no shiyo koka

    Energy Technology Data Exchange (ETDEWEB)

    Shoji, K.; Yoshihara, T. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1996-12-01

    The effect of synthetic plant hormone 4-C1-IAA and TFIBA on root growth in plantlet propagation was clarified by the cuttage and the issue culture of strawberry seedling production. A periwinkle, vine, and azalea are the effect of 4-C1-IAA on root growth, and a promotion effect was recognized for rooting and root elongation. The concentration of 4-C1-IAA in which the growth promotion effect of a root most appears varies depending on the species of a plant. The concentration of a periwinkle was 20 ppm, and that of an azalea was 2000 ppm. The growth promotion effect of a root in 4-C1-IAA and TFIBA was compared with IBA for an azalea. The result showed that 4-C1-IAA is the same in the effect as IBA and that TFIBA is higher than for IBA. The growth of a vine`s terminal bud was promoted by the effect of TFIBA on root growth, and the callus occurring when IBA was treated was not formed. The rooting of a strawberry was promoted by the effect of TFIBA on root growth. The combined use of TFIBA and BA promotes the growth of a side bud and forms a multi-bud plant. However, rooting was inhibited. The callus caused by the effect of BA on root growth could be suppressed through the combined use with TFIBA. 6 refs., 7 figs., 2 tabs.

  7. Somatic embryogenesis and plantlet regeneration in the genus Secale : 1. Somatic embryogenesis and organogenesis from cultured immature embryos of five wild species of rye.

    Science.gov (United States)

    Rybczyński, J J; Zduńczyk, W

    1986-12-01

    A tissue culture of five wild species of the Secale genus, i.e., S. africanum (Stapf.), S. ancestrale (Zhuk.), S. kuprianovii (Grossh), S. segetale (Rosher.), and S. vavilovii (Grossh), from immature embryos of sizes (stages) varying between 1.0 mm to 3.0mm, cultured on MS (1962) mineral nutrient medium supplemented with 0.62 mg/1-5.0 mg/1 of 2,4-D, was established. Initially various types of callus were observed and a correlation between genotype, size of explant and 2,4-D concentration was found. The best embryogenic response was observed when explants were smaller than 1.0 mm. Induction of somatic embryogenesis of 2.0 mm-3.0 mm explants required a higher concentration of 2,4-D. Most embryoids were formed in the presence of 5.0 mg/l of 2,4-D. Secale africanum and S. kuprianovii appeared to have the highest embryogenic capacity among the five investigated species. For embryoids germination to plantlets the MS medium supplemented with GA3 and cytokinins was used. Ultimately, out of the 932 regenerants obtained 364 originated from somatic embryogenesis.

  8. Establishing Symbito ic Cultivation System of Tissue -cultured Cy mbidium hyrb idum Plantlets and Endophy tic Fungi%杂交兰组培苗与内生真菌共生培养体系的构建

    Institute of Scientific and Technical Information of China (English)

    张晋彦; 吴争; 宁惠娟; 方载浩; 梁立军; 崔永一

    2016-01-01

    为了筛选出对杂交兰组培苗生长有益的菌株,采用4种内生真菌(被孢霉属Mortierella sp.),通过固体菌剂和液体菌剂2种接种方式,建立杂交兰组培苗与真菌的共生培养体系。共生培养150 d后,运用石蜡切片法观察到菌丝、菌丝结等典型的菌根结构。结合重分离真菌形态观察和分子鉴定,确定重分离菌株均为原接种菌株。结果表明:液体菌剂接种方式相比固体菌剂,对杂交兰组培苗生长的促进作用显著;菌株FC5和FC9液体菌剂对杂交兰组培苗的生长促进作用显著;FC17液体菌剂对杂交兰组培苗根部生长促进效果显著。%To screen out beneficial fungi strains to Cymbidiumh ybridum (C.hybridum‘Princess Nobuko’×C.goeringii‘Huan-gshuixian’ ) plantlets growth, using solid inoculation and liquid inoculation, we established mycorrhizal symbiosis system between four kinds of endophytic fungi ( Morit erell a sp).and Cymbidium hybridum plantlets.After 150-day symbiotic cul-ture, typical mycorrhizal structures such as hyphae and pelotons were founded by permanent paraffin-cut section method. Considering morphological observation and molecular identification, it was confirmed that fungi isolated from C.hybridum plantlets roots were the same as origin fungi.The liquid inoculum treatment was better than solid inoculum treatment. Strains FC5 and FC9 liquid inoculum treatments had obvious promoting effect on the growth of C.hybird um plantlets.Strain FC17 liquid inoculum treatments significantly promoted the growth of C.hybridum plantlet roots.

  9. Graviton as a Grand United Gauge Boson

    Science.gov (United States)

    Sharafiddinov, Rasulkhozha S.

    2015-04-01

    To any type of charge corresponds a kind of inertial mass. Such a mass-charge duality explains the availability in the neutrino of the naturally united rest mass and charge equal to all its mass and charge consisting of the electric, weak, strong and a range of other the innate components. From their point of view, we discuss a new theory of a grand unification. In this theory, the gravitational field is a naturally united field of the unified system of the most diverse combinations of the electric photons, magnetic monophotons, weak bosons and the strong gluons where the four pairs of forces of a different nature are united. Some consequences and laboratory confirmations of the discussed theory have been listed, in which graviton is predicted as a grand united gauge boson. Therefore, the gravitons constitute a natural light testifying in favor of gravitational force. They show that to each type of light corresponds a kind of force. Thereby, the suggested field theory that unites all gauge bosons in gravitons gives the possibility to directly look on the nature of gravitational matter elucidating the interratio of intragraviton forces and the problem of elementary particle fundamental symmetries.

  10. SUGRA Grand Unification, LHC and Dark Matter

    CERN Document Server

    Nath, Pran

    2012-01-01

    A brief review is given of recent developments related to the Higgs signal and its implications for supersymmetry in the supergravity grand unification framework. The Higgs data indicates that the allowed parameter space largely lies on focal curves and focal surfaces of the Hyperbolic Branch of radiative breaking of the electroweak symmetry where TeV size scalars naturally arise. The high mass of the Higgs leads to a more precise prediction for the allowed range of the spin independent neutralino -proton cross section which is encouraging for the detection of dark matter in future experiments with greater sensitivity. Also discussed is the status of grand unification and a natural solution to breaking the GUT group at one scale and resolving the doublet-triplet problem. It is shown that the cosmic coincidence can be compatible within a supersymmetric framework in a muticomponent dark matter picture with one component charged under $B-L$ while the other component is the conventional supersymmetric dark matter...

  11. Aspects Of Grand Unified And String Phenomenology

    CERN Document Server

    Walker, J W

    2005-01-01

    Explored in this report is the essential interconnectedness of Grand Unified and String Theoretic Phenomenology. In order to extract a modeled connection to low-energy physics from the context of superstring theory, it is presently necessary to input some preferred region of parameter space in which to search. This need may be well filled by a parallel study of Grand Unification, which is by contrast in immediate proximity to a wealth of experimental data. The favored GUT so isolated may then reasonably transfer this phenomenological correlation to a string embedding, receiving back by way of trade a greater sense of primary motivation, and potentially enhanced predictability for parameters taken as input in a particle physics context. The Flipped SU(5) GUT will be our preferred framework in which to operate and first receives an extended study in a non-string derived setting. Of particularly timely interest are predictions for super-particle mass ranges and the interrelated question of proton decay lifetime....

  12. Calculations of canonical averages from the grand canonical ensemble.

    Science.gov (United States)

    Kosov, D S; Gelin, M F; Vdovin, A I

    2008-02-01

    Grand canonical and canonical ensembles become equivalent in the thermodynamic limit, but when the system size is finite the results obtained in the two ensembles deviate from each other. In many important cases, the canonical ensemble provides an appropriate physical description but it is often much easier to perform the calculations in the corresponding grand canonical ensemble. We present a method to compute averages in the canonical ensemble based on calculations of the expectation values in the grand canonical ensemble. The number of particles, which is fixed in the canonical ensemble, is not necessarily the same as the average number of particles in the grand canonical ensemble.

  13. Re-examining the value of medical grand rounds.

    Science.gov (United States)

    Hebert, Randy S; Wright, Scott M

    2003-12-01

    To study medical grand rounds, the cornerstone of a department of medicine's educational programs. Between April and June 2001, a questionnaire was sent to chairs of departments of medicine at the 389 U.S. hospitals with medicine residency programs accredited by the Accreditation Council for Graduate Medical Education. Data were collected on the objectives and attendance, educational structure, perceived quality, and costs of medical grand rounds. Three hundred questionnaires were returned (77%). Grand rounds were offered by 97% of departments and accredited for continuing medical education in 96% of hospitals. The most important objectives were to educate, showcase faculty role models, and promote a collegial atmosphere. Patients were present at grand rounds less than 3% of the time. Grand rounds were predominantly lecture based; only 10% were clinical case presentations or interactive workshops/small groups, the formats proven most effective for facilitating adult learning and a humanistic approach to patients. Curricular tenets of needs assessment, program evaluation, and knowledge assessment were performed in only 73%, 59%, and 17% of programs, respectively. University hospitals were less likely to incorporate these principles (p <.01). Although respondents attested to the high quality of grand rounds, many potential attendees missed more than half the sessions. Grand rounds were the most expensive conference in 78% of departments, with the pharmaceutical industry providing the majority of the funding. Medical grand rounds are costly and often do not take into account learners' needs. Departments of medicine should reevaluate their commitment to grand rounds.

  14. La masa de los grandes impactores

    Science.gov (United States)

    Parisi, M. G.; Brunini, A.

    Los planetas han sido formados fundamentalmente acretando masa a través de colisiones con planetesimales sólidos. La masa más grande de la distribución de planetesimales y las masas máxima y mínima de los impactores, han sido calculadas usando los valores actuales del período y de la inclinación de los planetas (Lissauer & Safronov 1991; Parisi & Brunini 1996). Recientes investigaciones han mostrado, que las órbitas de los planetas gigantes no han sufrido variaciones con el tiempo, siendo su movimiento regular durante su evolución a partir de la finalización de la etapa de acreción (Laskar 1990, 1994). Por lo tanto, la eccentricidad actual de los planetas gigantes se puede utilizar para imponer una cota máxima a las masas y velocidades orbitales de los grandes impactores. Mediante un simple modelo dinámico, y considerando lo arriba mencionado, obtenemos la cota superior para la masa del planetesimal más grande que impactó a cada planeta gigante al final de su etapa de acreción. El resultado más importante de este trabajo es la estimación de la masa máxima permitida para impactar a Júpiter, la cúal es ~ 1.136 × 10 -1, siendo en el caso de Neptuno ~ 3.99 × 10 -2 (expresada en unidades de la masa final de cada planeta). Además, fue posible obtener la velocidad orbital máxima permitida para los impactores como una función de su masa, para cada planeta. Las cotas obtenidas para la masa y velocidad de los impactores de Saturno y Urano (en unidades de la masa y velocidad final de cada planeta respectivamente) son casi las mismas que las obtenidas para Júpiter debido a que estos tres planetas poseen similar eccentricidad actual. Nuestros resultados están en buen acuerdo con los obtenidos por Lissauer & Safronov (1991). Estas cotas podrían ser utilizadas para obtener la distribución de planetesimales en el Sistema Solar primitivo.

  15. A grand model for chemical product design

    DEFF Research Database (Denmark)

    Fung, Ka Y.; Ng, Ka M.; Zhang, Lei;

    2016-01-01

    Chemical engineering has been expanding its focus from primarily business-to-business products (B2B) to business-to-consumer (B2C) products. The production of B2B products generally emphasizes on process design and optimization, whereas the production of B2C products focuses on product quality......, ingredients and structure. Market and competitive analysis, government policies and regulations have to be explicitly considered in product design. All these considerations are accounted for in the Grand Product Design Model, which consists of a process model, a property model, a quality model, a cost model...... product composition changes with market conditions. Another is a hand lotion that illustrates how product quality affects the profit.(C) 2016 Elsevier Ltd. All rights reserved....

  16. Gauge-Higgs EW and Grand Unification

    Science.gov (United States)

    Hosotani, Yutaka

    Four-dimensional Higgs field is identified with the extra-dimensional component of gauge potentials in the gauge-Higgs unifiation scenario. SO(5) × U(1) gauge-Higgs EW unification in the Randall-Sundrum warped space is successful at low energies. The Higgs field appears as an Aharonov-Bohm phase θH in the fifth dimension. Its mass is generated at the quantum level and is finite. The model yields almost the same phenomenology as the standard model for θH bosons around 6-10 TeV with very broad widths. The scenario is generalized to SO(11) gauge-Higgs grand unification. Fermions are introduced in the spinor and vector representations of SO(11). Proton decay is naturally forbidden.

  17. Steroids from Dysoxylum grande (Meliaceae) leaves.

    Science.gov (United States)

    Wah, Low Kok; Abas, Faridah; Cordell, Geoffrey A; Ito, Hideyuki; Ismail, Intan Safinar

    2013-02-01

    Seven new 23-oxo-cholestane derivatives named as grandol A (1), B (2), C (3), D (4), E (5), F (6), and G (7) were isolated from Dysoxylum grande leaves alongside with a new 3,4-secodammar-4(28)-en-3-oic acid derivative (8). The structures of the compounds were elucidated based on the interpretation of spectroscopic data, and their relative configurations were established by NOESY 2D NMR data. All of the isolates were tested for anti-acetylcholinesterase activity using thin layer chromatography (TLC)-bioautography with fast blue B salt. Only grandol A (1) and B (2) showed positive results, with clear discoloration at a concentration of 12.5 ppm. However, the obtained IC(50) values for grandol A and B, when using Ellman's method, were not significant (>200 μg/ml).

  18. Las cubiertas de grandes luces. Soluciones actuales

    Directory of Open Access Journals (Sweden)

    de Alarcón Álvarez, C.

    1971-04-01

    Full Text Available This article deals generally with the problems connected with the construction of large span roofs, and also with the various types of designs that are currently in practice. It may be said that at present the only limitation to these designs is the imagination of the designer and the financial restrictions imposed on him.Se exponen en este artículo, de forma panorámica, los problemas que presenta la cobertura de grandes vanos y las diversas soluciones que se han adoptado hasta los tiempos actuales, en los que puede afirmarse que no existe más límite para la fantasía del proyectista que el condicionamiento del presupuesto de la obra.

  19. Grandes incendios urbanos: mesa redonda, Lima 2001

    Directory of Open Access Journals (Sweden)

    Juan L. Arce-Palomino

    2008-01-01

    Full Text Available Entre los desastres antrópicos destacan los grandes incendios urbanos. El 29 de diciembre de 2001, en un área comercial tugurizada del centro de Lima, Perú se produjo un gran incendio que causó 277 muertes, 247 heridos, millonarias pérdidas y un gran dolor en el pueblo peruano. Esta situación se había previsto, pero no se tomaron las acciones necesarias para evitarla. En este artículo se recopila la situación previa, las características de la tragedia, las acciones y organización que se tuvo para su control, se destaca el trabajo interdisciplinario y la una activa participación del Cuerpo General de Bomberos Voluntarios del Perú.

  20. Gauged flavor, supersymmetry and grand unification

    Science.gov (United States)

    Mohapatra, Rabindra N.

    2012-07-01

    I review a recent work on gauged flavor with left-right symmetry, where all masses and all Yukawa couplings owe their origin to spontaneous flavor symmetry breaking. This is suggested as a precursor to a full understanding of flavor of quarks and leptons. An essential ingredient of this approach is the existence of heavy vector-like fermions, which is the home of flavor, which subsequently gets transmitted to the familiar quarks and leptons via the seesaw mechanism. I then discuss implications of extending this idea to include supersymmetry and finally speculate on a possible grand unified model based on the gauge group SU(5)L×SU(5)R which provides a group theoretic origin for the vector-like fermions.

  1. Gauged Flavor, Supersymmetry and Grand Unification

    CERN Document Server

    Mohapatra, Rabindra N

    2012-01-01

    I review a recent work on gauged flavor with left-right symmetry, where all masses and all Yukawa couplings owe their origin to spontaneous flavor symmetry breaking. This is suggested as a precursor to a full understanding of flavor of quarks and leptons. An essential ingredient of this approach is the existence of heavy vector-like fermions, which is the home of flavor, which subsequently gets transmitted to the familiar quarks and leptons via the seesaw mechanism. I then discuss implications of extending this idea to include supersymmetry and finally speculate on a possible grand unified model based on the gauge group $SU(5)_L\\times SU(5)_R$ which provides a group theoretic origin for the vector-like fermions.

  2. Recent Results from Kascade-Grande

    CERN Document Server

    Kampert, K H; Ainsley, C; Åkesson, P F; Alexander, G; Anagnostou, G; Anderson, K J; Asai, S; Axen, D; Bailey, I; Barberio, E; Barillari, T; Barlow, R J; Batley, J Richard; Bechtle, P; Behnke, T; Bell, K W; Bell, P J; Bella, G; Bellerive, A; Benelli, G; Bethke, Siegfried; Biebel, O; Boeriu, O; Bock, P; Boutemeur, M; Braibant, S; Brown, R M; Burckhart, H J; Campana, S; Capiluppi, P; Carnegie, R K; Carter, A A; Carter, J R; Chang, C Y; Charlton, D G; Ciocca, C; Csilling, A; Cuani, M; Dado, S; de Roeck, A; De Wolf, E A; Desch, K; Dienes, B; Dubbert, J; Duchovni, E; Duckeck, G; Duerdoth, I P; Etzion, E; Fabbri, F; Ferrari, P; Fiedler, F; Fleck, I; Ford, M; Frey, A; Gagnon, P; Gary, J W; Geich-Gimbel, C; Giacomelli, G; Giacomelli, P; Giunta, M; Goldberg, J; Gross, E; Grunhaus, J; Gruwé, M; Sen-Gupta, A; Hajdu, C; Hamann, M; Hanson, G G; Harel, A; Hauschild, M; Hawkes, C M; Hawkings, R; Herten, G; Heuer, R D; Hill, J C; Horváth, D; Igo-Kemenes, P; Ishii, K; Jeremie, H; Jovanovic, P; Junk, T R; Kanzaki, J; Karlen, D; Kawagoe, K; Kawamoto, T; Keeler, R K; Kellogg, R G; Kennedy, B W; Kluth, S; Kobayashi, T; Kobel, M; Komamiya, S; Kramer, T; Krasznahorkay, A; Krieger, P; Von Krogh, J; Kühl, T; Kupper, M; Laerty, G D; Landsman, H; Lanske, D; Lellouch, D; Letts, J; Levinson, L; Lillich, J; Lloyd, S L; Loebinger, F K; Lü, J; Ludwig, A; Ludwig, J; Mader, W; Marcellini, S; Martin, A J; Mashimo, T; Mättig, P; McKenna, J; McPherson, R A; Meijers, F; Menges, W; Merritt, F S; Mes, H; Meyer, N; Michelini, A; Mihara, S; Mikenberg, G; Miller, D J; Mohr, W; Mori, T; Mutter, A; Nagai, K; Nakamura, I; Nanjo, H; Neal, H A; Nisius, R; O'Neale, S W; Oh, A; Oreglia, M J; Orito, S; Pahl, C; Pásztor, G; Pater, J R; Pilcher, J E; Pinfold, J L; Plane, D E; Pooth, O; Przybycien, M B; Quadt, A; Rabbertz, K; Rembser, C; Renkel, P; Roney, J M; Rossi, A M; Rozen, Y; Runge, K; Sachs, K; Saeki, T; Sarkisyan-Grinbaum, E; Schaile, A D; Schaile, O; Schar-Hansen, P; Schieck, J; Schörner-Sadenius, T; Schröder, M; Schumacher, M; Seuster, R; Shears, T G; Shen, B C; Sherwood, P; Skuja, A; Smith, A M; Sobie, R J; Söldner-Rembold, S; Spanó, F; Stahl, A; Strom, D; Ströhmer, R; Tarem, S; Tasevsky, M; Teuscher, R; Thomson, M A; Torrence, E; Toya, D; Tran, P; Trigger, I; Trócsányi, Z L; Tsur, E; Turner-Watson, M F; Ueda, I; Ujvári, B; Vollmer, C F; Vannerem, P; Vertesi, R; Verzocchi, M; Voss, H; Vossebeld, Joost Herman; Ward, C P; Ward, D R; Watkins, P M; Watson, A T; Watson, N K; Wells, P S; Wengler, T; Wermes, N; Wilson, G W; Wilson, J A; Wolf, G; Wyatt, T R; Yamashita, S; Zer-Zion, D; Zivkovic, L; Kampert, Karl-Heinz

    2006-01-01

    KASCADE-Grande is a new extensive air shower experiment co-located to the KASCADE site at Forschungszentrum Karlsruhe. The multi-detector system allows to investigate the energy spectrum, composition, and anisotropies of cosmic rays with unprecedented prevision in the energy range from 10^{14}-10^{18} eV. The primary goals besides investigating the origin of the knee at E ~ 3 * 10^{15} eV, are to verify the existence of the second knee at E ~ 10^{17} eV and to measure the composition in the expected transition region of galactic to extragalactic cosmic rays. The performance of the apparatus and shower reconstruction methods will be discussed on the basis of detailed Monte Carlo simulations and first data. First results based on slightly more than a year of data taking are presented.

  3. Gauge-Higgs EW and Grand Unification

    CERN Document Server

    Hosotani, Yutaka

    2016-01-01

    4D Higgs field is identified with the extra-dimensional component of gauge potentials in the gauge-Higgs unification scenario. $SO(5) \\times U(1)$ gauge-Higgs EW unification in the Randall-Sundrum warped space is successful at low energies. The Higgs field appears as an Aharonov-Bohm phase $\\theta_H$ in the fifth dimension. Its mass is generated at the quantum level and is finite. The model yields almost the same phenomenology as the standard model for $\\theta_H < 0.1$, and predicts $Z'$ bosons around 6 - 10 TeV with very broad widths. The scenario is genelarized to $SO(11)$ gauge-Higgs grand unification. Fermions are introduced in the spinor and vector representations of $SO(11)$. Proton decay is naturally forbidden.

  4. Grand Challenges in Protoplanetary Disc Modelling

    Science.gov (United States)

    Haworth, Thomas J.; Ilee, John D.; Forgan, Duncan H.; Facchini, Stefano; Price, Daniel J.; Boneberg, Dominika M.; Booth, Richard A.; Clarke, Cathie J.; Gonzalez, Jean-François; Hutchison, Mark A.; Kamp, Inga; Laibe, Guillaume; Lyra, Wladimir; Meru, Farzana; Mohanty, Subhanjoy; Panić, Olja; Rice, Ken; Suzuki, Takeru; Teague, Richard; Walsh, Catherine; Woitke, Peter; Community authors

    2016-10-01

    The Protoplanetary Discussions conference-held in Edinburgh, UK, from 2016 March 7th-11th-included several open sessions led by participants. This paper reports on the discussions collectively concerned with the multi-physics modelling of protoplanetary discs, including the self-consistent calculation of gas and dust dynamics, radiative transfer, and chemistry. After a short introduction to each of these disciplines in isolation, we identify a series of burning questions and grand challenges associated with their continuing development and integration. We then discuss potential pathways towards solving these challenges, grouped by strategical, technical, and collaborative developments. This paper is not intended to be a review, but rather to motivate and direct future research and collaboration across typically distinct fields based on community-driven input, to encourage further progress in our understanding of circumstellar and protoplanetary discs.

  5. Grand challenges in protoplanetary disc modelling

    CERN Document Server

    Haworth, Thomas J; Forgan, Duncan H; Facchini, Stefano; Price, Daniel J; authors, Community; Boneberg, Dominika M; Booth, Richard A; Gonzalez, Jean-François; Hutchison, Mark A; Laibe, Guillaume; Meru, Farzana; Mohanty, Subhanjoy; Panić, Olja; Rice, Ken; Walsh, Catherine; Woitke, Peter

    2016-01-01

    The Protoplanetary Discussions conference --- held in Edinburgh, UK, from 7th-11th March 2016 --- included several open sessions led by participants. This paper reports on the discussions collectively concerned with the multiphysics modelling of protoplanetary discs, including the self-consistent calculation of gas and dust dynamics, radiative transfer and chemistry. After a short introduction to each of these disciplines in isolation, we identify a series of burning questions and grand challenges associated with their integration, which are not necessarily achievable in the short-to-medium term. We then discuss potential pathways towards solving these challenges, grouped by strategical, technical and collaborative developments. This paper is not intended to be a review, but rather to motivate and direct future research and collaboration across typically distinct fields based on community driven input, to encourage further progress in our understanding of circumstellar and protoplanetary discs.

  6. The ten grand challenges of synthetic life.

    Science.gov (United States)

    Porcar, Manuel; Danchin, Antoine; de Lorenzo, Victor; Dos Santos, Vitor A; Krasnogor, Natalio; Rasmussen, Steen; Moya, Andrés

    2011-06-01

    The construction of artificial life is one of the main scientific challenges of the Synthetic Biology era. Advances in DNA synthesis and a better understanding of regulatory processes make the goal of constructing the first artificial cell a realistic possibility. This would be both a fundamental scientific milestone and a starting point of a vast range of applications, from biofuel production to drug design. However, several major issues might hamper the objective of achieving an artificial cell. From the bottom-up to the selection-based strategies, this work encompasses the ten grand challenges synthetic biologists will have to be aware of in order to cope with the task of creating life in the lab.

  7. A unified grand tour of theoretical physics

    CERN Document Server

    Lawrie, Ian D

    2013-01-01

    A Unified Grand Tour of Theoretical Physics invites its readers to a guided exploration of the theoretical ideas that shape our contemporary understanding of the physical world at the fundamental level. Its central themes, comprising space-time geometry and the general relativistic account of gravity, quantum field theory and the gauge theories of fundamental forces, and statistical mechanics and the theory of phase transitions, are developed in explicit mathematical detail, with an emphasis on conceptual understanding. Straightforward treatments of the standard models of particle physics and cosmology are supplemented with introductory accounts of more speculative theories, including supersymmetry and string theory. This third edition of the Tour includes a new chapter on quantum gravity, focusing on the approach known as Loop Quantum Gravity, while new sections provide extended discussions of topics that have become prominent in recent years, such as the Higgs boson, massive neutrinos, cosmological perturba...

  8. Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

    OpenAIRE

    Rong-Jyh Lin; Mei-Hsuan Wu; Yi-Hsuan Ma; Li-Yu Chung; Chung-Yi Chen; Chuan-Min Yen

    2014-01-01

    Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemeri...

  9. Multiple formation mechanisms of ferrous olivine in CV carbonaceous chondrites during fluid-assisted metamorphism

    OpenAIRE

    Krot, Alexander N.; Petaev,Michail I.; Bland, Phil A.

    2004-01-01

    The CV carbonaceous chondrites experienced alteration that resulted in formation of secondary ferrous olivine (Fa40-100), salite-hedenbergite pyroxenes (Fs10-50Wo45-50), wollastonite, andradite, nepheline, sodalite, phyllosilicates, magnetite, Fe,Ni-sulfides and Ni-rich metal in their Ca,Al-rich inclusions, amoeboid olivine ag-gregates, chondrules, and matrices. It has previously been suggested that fibrous ferrous olivine in dark inclusions in CV chondrites formed by dehydration of phyllosil...

  10. Plug-in de procesado visual (OpenCV) en OpenDomo OS

    OpenAIRE

    Herrera Castro, Alexander

    2015-01-01

    Este proyecto consiste en el diseño y desarrollo de un plug-in que permita usar el sistema de procesado de imagen OpenCV desde el sistema operativo OpenDomo OS. Aquest projecte consisteix en el disseny i desenvolupament d'un plug-in que permeti utilitzar el sistema de processament d'imatge OpenCV des del sistema operatiu OpenDomo OS. Master thesis for the Free Software program.

  11. Biofuels from the Fresh Water Microalgae Chlorella vulgaris (FWM-CV for Diesel Engines

    Directory of Open Access Journals (Sweden)

    Saddam H. Al-lwayzy

    2014-03-01

    Full Text Available This work aims to investigate biofuels for diesel engines produced on a lab-scale from the fresh water microalgae Chlorella vulgaris (FWM-CV. The impact of growing conditions on the properties of biodiesel produced from FWM-CV was evaluated. The properties of FWM-CV biodiesel were found to be within the ASTM standards for biodiesel. Due to the limited amount of biodiesel produced on the lab-scale, the biomass of dry cells of FWM-CV was used to yield emulsified water fuel. The preparation of emulsion fuel with and without FWM-CV cells was conducted using ultrasound to overcome the problems of large size microalgae colonies and to form homogenized emulsions. The emulsified water fuels, prepared using ultrasound, were found to be stable and the size of FWM-CV colonies were effectively reduced to pass through the engine nozzle safely. Engine tests at 3670 rpm were conducted using three fuels: cottonseed biodiesel CS-B100, emulsified cottonseed biodiesel water fuel, water and emulsifier (CS-E20 and emulsified water containing FWM-CV cells CS-ME20. The results showed that the brake specific fuel consumption (BSFC was increased by about 41% when the engine was fueled with emulsified water fuels compared to CS-B100. The engine power, exhaust gas temperature, NOx and CO2 were significantly lower than that produced by CS-B100. The CS-ME20 produced higher power than CS-E20 due to the heating value improvement as a result of adding FWM-CV cells to the fuel.

  12. Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

    OpenAIRE

    Lin, Rong-Jyh; Wu, Mei-Hsuan; Ma, Yi-Hsuan; Chung, Li-Yu; Chen, Chung-Yi; Yen, Chuan-Min

    2014-01-01

    Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (...

  13. Chamobtusin A, a novel skeleton diterpenoid alkaloid from Chamaecyparis obtusa cv. tetragon.

    Science.gov (United States)

    Zhang, Yu-Mei; Tan, Ning-Hua; Lu, Yang; Chang, Ying; Jia, Rui-Rui

    2007-10-25

    The novel diterpenoid alkaloid chamobtusin A (1) was isolated from the branches and leaves of Chamaecyparis obtusa cv. tetragon. Its structure and relative stereochemistry were mainly determined by MS, 2D NMR, and X-ray methods. The methanol extracts, total alkaloids of C. obtusa cv. tetragon, and chamobtusin A were tested for their cytotoxicities against A549 and K562 human tumor cell lines.

  14. Working with OpenCV and Intel Image Proccessing Libraries. Proccessing image data tools

    OpenAIRE

    Mora Lizán, Francisco José; Llorens Largo, Faraón; Pujol López, Mar; Rizo Aldeguer, Ramón; Villagrá Arnedo, Carlos

    2002-01-01

    We will provide an overview of Intel OpenCV and Image Processing Libraries. We present an application of real-time gesture recognition using the libraries (segmenting a foreground object, creating Motion History Image (MHI), updating the intensity gradients, and recovering directional motion information). Some times most companies spent a lot of time and money implementing those well-known techniques. OpenCV and IPL implement a huge amount of standard and advanced image processing techniqu...

  15. 36 CFR 7.22 - Grand Teton National Park.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Grand Teton National Park. 7.22 Section 7.22 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR SPECIAL REGULATIONS, AREAS OF THE NATIONAL PARK SYSTEM § 7.22 Grand Teton National Park. (a)...

  16. 36 CFR 7.4 - Grand Canyon National Park.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Grand Canyon National Park. 7.4 Section 7.4 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR SPECIAL REGULATIONS, AREAS OF THE NATIONAL PARK SYSTEM § 7.4 Grand Canyon National Park. (a)...

  17. Grand Hyatt Beijing Opens Wine Shop in the Patisserie

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    Over the years,Grand Hyatt Beijing has become a crowd favorite for wine events thanks to events such as its many exquisite wine dinners at the hotel’s superb Italian restaurant Da Giorgio,the Wine Club at Redmoon and the Grand Wine Sale at the Patisserie."The wine shop will cater to Beijing’s growing community of wine

  18. Gauge coupling unification in gauge-Higgs grand unification

    Science.gov (United States)

    Yamatsu, Naoki

    2016-04-01

    We discuss renormalization group equations for gauge coupling constants in gauge-Higgs grand unification on five-dimensional Randall-Sundrum warped space. We show that all four-dimensional Standard Model gauge coupling constants are asymptotically free and are effectively unified in SO(11) gauge-Higgs grand unified theories on 5D Randall-Sundrum warped space.

  19. Elastoplasticidad anisotropa de metales en grandes deformaciones

    Science.gov (United States)

    Caminero Torija, Miguel Angel

    El objetivo de este trabajo es el desarrollo de modelos y algoritmos numericos que simulen el comportamiento del material bajo estas condiciones en el contexto de programas de elementos finitos, dando como resultado predicciones mas precisas de los procesos de conformado y deformacion plastica en general. Para lograr este objetivo se han desarrollado diversas tareas destinadas a mejorar las predicciones en tres aspectos fundamentales. El primer aspecto consiste en la mejora de la descripcion del endurecimiento cinematico anisotropo en pequenas deformaciones, lo cual se ha realizado a traves de modelos y algoritmos implicitos de superficies multiples. Ha sido estudiada la consistencia de este tipo de modelos tanto si estan basados en una regla implicita similar a la de Mroz o en la regla de Prager. Ademas se han simulado los ensayos de Lamba y Sidebottom, obteniendo, en contra de la creencia general, muy buenas predicciones con la regla de Prager. Dichos modelos podrian ser extendidos de forma relativamente facil para considerar grandes deformaciones a traves de procedimientos en deformaciones logaritmicas, similares a los desarrollados en esta tesis y detallados a continuacion. El segundo aspecto consiste en la descripcion de la anisotropia elastoplastica inicial. Esto se ha conseguido mediante el desarrollo de modelos y algoritmos para plasticidad anisotropa en grandes deformaciones, bien ignorando la posible anisotropia elastica, bien considerandola simultaneamente con la anisotropia plastica. Para ello ha sido necesario desarrollar primero un nuevo algoritmo de elastoplasticidad anisotropa en pequenas deformaciones consistentemente linealizado y sin despreciar ningun termino, de tal forma que se conserve la convergencia cuadratica de los metodos de Newton. Este algoritmo en pequenas deformaciones ha servido para realizar la correccion plastica de dos algoritmos en grandes deformaciones. El primero de estos algoritmos es una variacion del clasico algoritmo de

  20. In vitro somatic embryogenesis and plantlet regeneration from immature male inflorescence of adult dura and tenera palms of Elaeis guineensis (Jacq.).

    Science.gov (United States)

    Jayanthi, Madhavan; Susanthi, Bollarapu; Murali Mohan, Nandiganti; Mandal, Pranab Kumar

    2015-01-01

    We report here a method for plant regeneration through somatic embryogenesis from explants collected from immature male inflorescence of adult oil palm cultivated in India. Callus induction was successful from tissues of immature male inflorescence collected from both dura and tenera varieties of oil palm. A modified Y3 (Eeuwens) media supplemented with several additives and activated charcoal (3%) were used for the experiments. Out of four different auxin treatments, 4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid (picloram) produced maximum callus induction (82%) and it was not significantly different from 2,4-dichlorophenoxyacetic acid (2,4-D) and a combination of 2,4-D + picloram. The callus induction obtained with auxin α-naphthalene acetic acid was only 54% and it was significantly low as compared to the other treatments. Highest embryogenesis was obtained with a combination of 2,4-D + picloram (4.9%) followed by picloram (3.4%). Genotypic variation in response to the same auxins was observed both for callus induction and embryogenesis. Callus induction and embryogenesis ranged from 42 to 72% and 6.8 to 9.35%, respectively in tenera. The formation of embryogenic calli was marked by the appearance of white to yellowish globular or nodular structures which subsequently formed clear somatic embryos. Somatic embryogenesis was asynchronous and at one time we could find different stages of embryogenesis like the globular, torpedo and the cotyledonary stages. The somatic embryos when exposed to light in the same basal media along with 6-benzyladenine (18 µM), abscisic acid (3.78 µM) and gibberellic acid (5.78 µM) regenerated into plantlets. To the best of our knowledge this is the first report o f callus induction and somatic embryogenesis from immature male inflorescence of oil palm.

  1. Modeling the Galactic CV Distribution for the ChaMPlane Survey

    CERN Document Server

    Rogel, A B; Lugger, P M

    2007-01-01

    For purposes of designing targeted cataclysmic variable (CV) detection surveys and interpreting results of other projects with many CV detections such as the ChaMPlane Survey, we have created a model of the CV distribution in the Galaxy. It is modeled as a warped, flared exponential disk with a gaussian vertical distribution. Extinction is based on a detailed Galactic dust and gas model. A luminosity function for CVs is also incorporated, based on a smoothed version of published data. We calculate predicted field detection rates as a function of the limiting magnitude expected for the detecting system (i.e. WIYN/Hydra or NOAO 4m/Mosaic). Monte-Carlo techniques are used to assess statistical fluctuations in these rates. We have created maps of the expected CV distribution for the full non-bulge Galactic plane (20CV surveys. Assuming a CV distribution with a scale height of 160 pc, the ChaMPlane observational result of 5 CVs in 13 northern...

  2. Evaluation of analgesic, anti-inflammatory, anti-depressant and anti-coagulant properties of Lactuca sativa (CV. Grand Rapids) plant tissues and cell suspension in rats.

    Science.gov (United States)

    Ismail, Hammad; Mirza, Bushra

    2015-06-27

    Lactuca sativa (lettuce) has been traditionally used for relieving pain, inflammation, stomach problems including indigestion and lack of appetite. Moreover, the therapeutic significance of L. sativa includes its anticonvulsant, sedative-hypnotic and antioxidant properties. In the present study, the MC (methanol and chloroform; 1:1) and aqueous extracts of seed and leaf along with cell suspension exudate were prepared. These extracts were explored for their analgesic, anti-inflammatory, antidepressant and anticoagulant effects by hot plate analgesic assay; carrageenan induced hind paw edema test, forced swimming test and capillary method for blood clotting respectively in a rat model. The results were analyzed using one-way Analysis of Variance (ANOVA) followed by Turkey multiple comparison test. Interestingly, the extracts and the cell suspension exudate showed dual inhibition by reducing pain and inflammation. The results indicated that the aqueous extracts of leaf exhibited highest analgesic and anti-inflammatory activities followed by leaf MC, cell suspension exudate, seed aqueous and seed MC extracts. The current findings show that aqueous and MC extracts of seed have the least immobility time in the forced swimming test, which could act as an anti-depressant on the central nervous system. The leaf extracts and cell suspension exudate also expressed moderate anti-depressant activities. In anticoagulant assay, the coagulation time of aspirin (positive control) and MC extract of leaf was comparable, suggesting strong anti-coagulant effect. Additionally, no abnormal behavior or lethality was observed in any animal tested. Taken together, L. sativa can potentially act as a strong herbal drug due to its multiple pharmaceutical effects and is therefore of interest in drug discovery and development of formulations.

  3. 昼夜温差对文心兰试管苗生长的影响%Effects of Day-night Temperature Difference on the Growth of Oncidium Plantlets in vitro

    Institute of Scientific and Technical Information of China (English)

    郑卫杰; 郭子霞; 王政; 何松林

    2011-01-01

    The plantlets of Oncidium var.‘Ning Meng Huang' in vitro were used as experimental materials to study the effects of day-night temperature differences on its growth.The results showed that the index of plantlets in vitro under the treatment that the day-night temperature difference was 3℃, including plant height, leaf length, fresh weight and dry weight of the total and shoot part, were the best than other treatment, and there was significant difference among the other treatments.The root number, fresh weight and dry weight of the root part of plantlets in vitro were better than others under the treatment that the day-night temperature difference was 12℃, and the soluble sugar and root activity of plantlets in vitro were also the biggest one.It was an increasing tendency, as the chlorophyll a, chlorophyll b, total leaf chlorophyll content and dry matter rate of plantlets in vitro, with the increasing of day-night temperature difference and then was highest one under the treatment that day-night temperature difference was 15℃.Overall, the morphological growth of Oncidium plantlets in vitro was best under the treatment that the day-night temperature difference was 3℃, the root grew better under the treatment that the day-night temperature difference was 12℃ than others, and when the day-nigh difference was 15℃, it was the best treatment for benefiting the dry material accumulation and the synthesis of chlorophyll.%以文心兰‘柠檬黄'试管苗为材料,探讨了不同昼夜温差对其生长的影响.结果表明:昼夜温差3℃处理下文心兰试管苗的株高、叶长、总鲜重、地上部鲜重、总干重、地上部干重均达到最大值,且与其他处理差异性显著.昼夜温差12℃时文心兰试管苗根数、最大根长、地下部鲜重及干重最大,且在此处理下可溶性糖含量和根系活力也达到最大值.叶绿素a、叶绿素b、总叶绿素含量和干物率均随着昼夜温差的不断增大呈现出增

  4. 75 FR 13138 - Grand Ditch Breach Restoration Environmental Impact Statement, Rocky Mountain National Park, CO

    Science.gov (United States)

    2010-03-18

    ... National Park Service Grand Ditch Breach Restoration Environmental Impact Statement, Rocky Mountain... prepare an Environmental Impact Statement for the Grand Ditch Breach Restoration, Rocky Mountain National...), the National Park Service is preparing an Environmental Impact Statement for the Grand Ditch...

  5. 不同水平腐胺对巴西蕉幼苗抗旱生理的影响%Effects of Putrescine at Different Levels on the Drought Resistance of Brazil Banana Plantlets

    Institute of Scientific and Technical Information of China (English)

    王晶晶; 李绍鹏; 李新国; 吴凡; 李茂富; 黄芳; 何娟

    2011-01-01

    Foliar spraying of Brazil banana plantlets with 0.00, 1.00, 1.25, 1.50 and 1.75 ramol/L putrescine and then treated under drought stress, some relative physiological and biochemical indices were measured to study the changes. The results showed that the pretreatment with suitable concentrations of exogenous putrescine increased the relative water content of the Brazil banana plantlet leaves, reduced the relative conductivity, improved the contents of proline and soluble proteins, as well as the activity of SOD and POD, decreased the rate of O2-production and the content of MDA. The results proved that the suitable concentrations of exogenous putrescine could improve the drought resistance of Brazil banana plantlets. The optimal putrescine concentration was found to be 1.25 mmol/L.%用0.00、1.00、1.25、1.50、1.75 mmol/L腐胺(Put)喷施巴西蕉幼苗叶片,研究干旱胁迫后其相关生理生化指标的变化.结果表明,在干旱胁迫下,适宜浓度的外源Put预处理能显著增加巴西蕉幼苗叶片的相对含水量,降低相对电导率,提高脯氨酸、可溶性蛋白质的含量及S0D、POD的活性,.降低O2产生的速率,减少MDA的积累.由此说明适宜浓度的外源Put预处理能提高巴西蕉幼苗抗旱性,以1.25 mmol/L Put预处理效应最佳.

  6. 光照强度和水质对马铃薯脱毒试管苗生长的影响%Effects of Light Intensity and Water Quality on Growth of in vitro Plantlets of Potato

    Institute of Scientific and Technical Information of China (English)

    李润; 刘绍文; 潘俊锋; 郭蓓蓓; 曾莉; 王燕; 肖勇; 杨丽

    2013-01-01

    研究光照强度、品种和水质对马铃薯试管苗生长的影响,为降低脱毒试管苗的扩繁成本及组培技术的优化提供参考。结果表明:14 h/d光照、强度为2000 lx,是马铃薯脱毒试管苗生长的最优光照条件,其次为自然光条件;在相同的生长条件下,品种‘黔芋1号’各农艺性状都优于‘青薯9号’;水质除叶片数和茎粗外,对其他农艺性状影响都不显著,即在生产过程中可用自来水代替蒸馏水,从而降低生产成本。%Light intensity, variety, and water quality were studied for their influence on the growth of plantlets invitroof potato in order to provide a reference for reducing cost and optimizing tissue culture technology. The results showed that the best light condition for growth of plantlets invitro was 14 h/d and light intensity 2 000 lx, and then was natural light;the agronomic traits of 'Qianyu 1' were better than 'Qingshu 9';water quality only had a significant impact on leaf number and stem diameter, but had no influence on other agronomic traits, and therefore, tap water instead of distil ed water should be used in plantlets in vitro production.

  7. 不同基质对香蕉组培苗生长及光合特性的影响%The Effects of the Different Substrates on Banana Plantlet Growth and Photosynthetic Characteristics

    Institute of Scientific and Technical Information of China (English)

    王必尊; 唐粉玲; 何应对; 刘永霞; 曹红鑫; 臧小平; 周兆禧; 韩丽娜; 郑丽丽

    2013-01-01

    The paper was aimed to screen suitable culture medium from six culture media of different ratio between coconut coir and lateritic soil for banana plantlet .Brazil Banana plantlet was selected and lateritic soil was used as the reference. The Li-6400 photosynthesis instrument was used to measure the diurnal variation of photosynthetic rate and transpiration rate.The result showed that the change of photosynthetic rate, transpiration rate and stomatal conductance was similar under different culture medium. When the ratio between coconut coir and lateritic soil was 1:2, the growth of banana plantlet was the best, and the above measured values were the highest; when the ratio was 4:1, the result was the worst. The intracellular concentration of CO2 was in the opposite.The growth of banana plantlet was the best when the ratio between coconut coir and lateritic soil was 1 : 2.%用椰糠与砖红壤土配成的6种不同基质栽培巴西香蕉组培苗,进行生长效果的比较;采用Li-6400便携式光合测定仪测定组培苗的净光合速率(pn)、蒸腾速率Tr等的日变化,通过相关性分析不同光合参数对净光合速率(pn)日变化的影响.结果表明:以椰糠:壤土=1:2配方对组培苗的生长效果最好;组培苗在不同基质下的净光合速率(pn)、蒸腾速率、气孔导度等变化趋势相似,其中以椰糠:壤土=1:2处理的数值最高,椰糠:壤土=4:1最低,胞间CO2浓度的数值则相反.6种基质栽培的香蕉组培苗以T2,即椰糠:壤土=1:2配制最适合香蕉组培苗生长.

  8. 外源5-氨基乙酰丙酸对红香芋试管苗耐冷性的影响%Effect of Exogenous 5 -aminolevulinic Acid on Cold Tolerance of Taro Plantlets in vitro

    Institute of Scientific and Technical Information of China (English)

    何继红; 蒋芳玲; 李洁英; 吴震

    2012-01-01

    The effects of different concentrations of exogenous 5 - aminolevulinic acid ( ALA) on the cold tolerance and related physiological indexes of taro [ Colocasia esculenta ( L. ) Schott ] plantlets in vitro were studied under the low temperature stress simulated artificially. The results showed that: under short -term (16 h) low temperature stress, the 2 ~20 mg/L exogenous ALA treatment could reduce the damaged degree of taro plantlets in vitro, promote the activities of superoxide dismutase ( SOD) , peroxidase ( POD) , catalase ( CAT) and ascorbic acid peroxidase ( APX) , decrease the production speed of superoxide anion ( · O2 -) and the content of hydrogen peroxide ( H2 O2 ) , suppress the increase in malonaldehyde ( MDA) content and plasma membrane permeability, and strengthen the ability of cell osmoregulation. The above results indicated that the ALA with suitable concentration could enhance the cold tolerance of taro plantlets in vitro, and relieve the injury of low temperature stress to taro test -tube plantlets. The application of 10 mg/L ALA could obtain the best effect.%为探讨外源5 -氨基乙酰丙酸对芋试管苗耐冷性的影响,以红香芋为试材,通过人工模拟低温逆境,研究了不同浓度外源ALA处理下芋试管苗耐冷性相关生理指标的变化.结果表明:短期(16 h)低温胁迫下,添加2~20 mg/L外源ALA可减轻芋试管苗冷害程度,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,降低超氧阴离子(·O2-)产生速率和过氧化氢( H2O2)含量,抑制丙二醛(MDA)含量和质膜透性的上升,同时增强细胞渗透调节能力.以上结果说明,适宜浓度的ALA处理可缓解低温胁迫对芋试管苗的伤害,提高其耐冷性;ALA处理浓度以10 mg/L效果最好.

  9. Mastoplastia reductora en L para grandes volúmenes

    OpenAIRE

    Fernandez, Claudio de Barros

    2013-01-01

    Las primeras descripciones de las mastoplastias fueron atribuidas a Paulus Aegineta en 625-690d.C. 4, con una resección de ginecomastia por el surco sub-mamario. Posteriormente, muchas técnicas fueron descritas para la disminución del órgano. Clásicamente, fueron consideradas mamas grandes aquellas con volúmenes resecados más grandes que 600 gr., siendo la mayoría tratadas tras cicatrices en T invertido. El objetivo de ese estudio es mostrar que es posible tratar mamas grandes con cicatrices ...

  10. Mastoplastia reductora en l para grandes volúmenes

    OpenAIRE

    De Barros Fernandez, Claudio

    2012-01-01

    Las primeras descripciones de las mastoplastias fueron atribuidas a Paulus Aegineta en 625-690d.C. 4, con una resección de ginecomastia por el surco sub-mamario. Posteriormente, muchas técnicas fueron descritas para la disminución del órgano. Clásicamente, fueron consideradas mamas grandes aquellas con volúmenes resecados más grandes que 600 gr., siendo la mayoría tratadas tras cicatrices en T invertido. El objetivo de ese estudio es mostrar que es posible tratar mamas grandes ...

  11. Water resources of the Grand Rapids area, Michigan

    Science.gov (United States)

    Stramel, G.J.; Wisler, C.O.; Laird, L.B.

    1954-01-01

    The Grand Rapids area, Michigan, has three sources from which to obtain its water supply: Lake Michigan, the Grand River and its tributaries, and ground water. Each of the first two and possibly the third is capable of supplying the entire needs of the area.This area is now obtaining a part of its supply from each of these sources. Of the average use of 50 mgd (million gallons per day) during 1951, Lake Michigan supplied 29 mgd; the Grand River and its tributaries supplied 1 mgd; and ground water supplied 20 mgd.Lake Michigan offers a practically unlimited source of potable water. However, the cost of delivery to the Grand Rapids area presents an economic problem in the further development of this source. Even without storage the Grand River can provide an adequate supply for the city of Grand Rapids. The present average use of the city of Grand Rapids is about 30 mgd and the maximum use is about 60 mgd, while the average flow of the Grand River is 2, 495 mgd or 3, 860 cfs (cubic feet per second) and the minimum daily flow recorded is 246 mgd. The quality and temperature of water in the Grand River is less desirable than Lake Michigan water. However, with proper treatment its chemical quality can be made entirely satisfactory.The city of Grand Rapids is actively engaged in a study that will lead to the expansion of its present water-supply facilities to meet the expected growth in population in Grand Rapids and its environs.Ground-water aquifers in the area are a large potential source of supply. The Grand Rapids area is underlain by glacial material containing a moderately hard to very hard water of varying chemical composition but suitable for most uses. The glacial outwash and lacustrine deposits bordering principal streams afford the greatest potential for the development of large supplies of potable ground water. Below the glacial drift, bedrock formations contain water that is extremely hard and moderately to highly mineralized. Thus the major sources of

  12. The People-First Service of Grand Hotel Beijing

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Grand Hotel Beijing is located north of Chang’an Avenue and east of the landmark Forbidden City in Beijing.This five-star luxury hotel has received numerous government delegations and state leaders from around the world since it opened in 1990.Wang Fuhe has been General Manager of Grand Hotel Beijing for more than 10 years.Through the effortsofWangandhisteam,thehotelflagshipsteers steady and fast,taking a leading position among the countless luxury hotels in Beijing.Wang attributes the success of Grand Hotel Beijing to its distinctive

  13. El cólera: una enfermedad infecciosa reemergente. El candidato vacunal cv 638, una herramienta para su prevención

    Directory of Open Access Journals (Sweden)

    Adrian Zelada-Valdés

    2015-01-01

    Full Text Available Este artículo tiene como objetivo realizar una revisión de la enfermedad del cólera y del candidato vacunal CV 638 . El cólera es una en fermedad infecciosa reemergente , caracterizada por diarreas profusas, con apariencia de agua de arroz, causada por la ingestión de alimentos o agua s contaminada s con la bacteria Vibrio cholerae de los serogrupos O1 y O139. El serogrupo O1 es el causante de la séptima pandemia. La incidencia de diversos factores como son los desastres naturales, sistemas sanitarios deficientes, la poca accesibilidad al agua potable, ha favorecido a que cada año se reporte n entre 3 y 5 millones de personas que sufren esta enfermedad, con una alta cif ra de defunciones . Además, en muchas regiones del planeta, el cólera se ha vuelto endémico, lo que ha provocado grandes pérdidas en la macro y microeconomía de los países afectados. Asimismo, se ha reportado un aumento de la resistencia a los agentes antim icrobian os usados tradi cionalmente para combatir la enf ermedad. Por tal razón, desde el año 2005 la Organización Mundial de la Salud ha recomendado el uso de vacunas orales como una herramienta para el control de la enfermedad en ciertas situaciones endémicas y epidémicas. En Cuba, a raíz de la epidemia de cólera que azotó a Perú en 1991, se obtuvo el candidato vacunal CV 638, el cual ha demostrado que es seguro, inmunogénico y protector

  14. Produtividade e valor nutritivo da Brachiaria brizantha cv. Marandu em um sistema silvipastoril Productivity and nutritional value of Brachiaria brizantha cv. Marandu in a silvopastoral system

    Directory of Open Access Journals (Sweden)

    L.F. Sousa

    2007-08-01

    Full Text Available O experimento foi realizado em sistema silvipastoril (Ssp localizado no bioma de Cerrado, no município de Lagoa Santa, Minas Gerais, no ano agrícola de 2004-2005. Avaliou-se a influência do Ssp composto pela arbórea bolsa-de-pastor (Zeyheria tuberculosa sobre os parâmetros produção, atributos morfogênicos, composição bromatológica e valor nutritivo da gramínea Brachiaria brizantha cv. Marandu, e os efeitos sobre as condições microclimáticas. O delineamento experimental foi em blocos ao acaso, com cinco blocos (épocas de corte, dois tratamentos (T1 - B. brizantha cv. Marandu sombreada com a Z. tuberculosa e T2 - B. brizantha cv. Marandu, a pleno sol e três repetições (parcelas. O sombreamento proporcionado pela Z. tuberculosa, apesar de reduzir a produção de matéria seca (MS da gramínea, favoreceu o aumento do teor de proteina bruta (PB e não alterou a produção de PB/ha e nem afetou a concentração de FDN. Os teores de FDA foram mais altos que os encontrados a pleno sol, o que provavelmente resultou em menor degradabilidade in vitro da MS e da matéria orgânica, não alterando a degradabilidade efetiva.The experiment was carried out in a Silvopastoral system (Sps located in Brazilian Savannah, Lagoa Santa county, Minas Gerais State, during the agricultural year of 2004-2005. It was studied the influence of Sps formed by "Bolsa de Pastor" (Zeyheria tuberculosa on the production, morphogenic attributes, bromatological composition and nutritional value of the graminaceous Brachiaria brizantha cv. Marandu as well as on the microclimatic aspects. The Sps was established in 1982, in a red-yellow latossol by the natural regeneration process. The experiment was based on a randomized block design, with 5 blocks (cutting times, 2 treatments (T1 - B. brizantha cv. Marandu shaded by Z. tuberculosa and T2 - B. brizantha cv. Marandu at open sky and three repetitions (plots. Although the DM production of the graminaceous in the Sps

  15. Toward realistic gauge-Higgs grand unification

    Science.gov (United States)

    Furui, Atsushi; Hosotani, Yutaka; Yamatsu, Naoki

    2016-09-01

    The SO(11) gauge-Higgs grand unification in the Randall-Sundrum warped space is presented. The 4D Higgs field is identified as the zero mode of the fifth-dimensional component of the gauge potentials, or as the fluctuation mode of the Aharonov-Bohm phase θ along the fifth dimension. Fermions are introduced in the bulk in the spinor and vector representations of SO(11). SO(11) is broken to SO(4)×SO(6) by the orbifold boundary conditions, which is broken to SU2×U1×SU3 by a brane scalar. Evaluating the effective potential V(θ), we show that the electroweak symmetry is dynamically broken to U1. The quark-lepton masses are generated by the Hosotani mechanism and brane interactions, with which the observed mass spectrum is reproduced. Proton decay is forbidden thanks to the new fermion number conservation. It is pointed out that there appear light exotic fermions. The Higgs boson mass is determined with the quark-lepton masses given; however, it turns out to be smaller than the observed value.

  16. desenvolvimento para o Rio Grande do Sul

    Directory of Open Access Journals (Sweden)

    Benedito Silva Neto

    2005-01-01

    Full Text Available The paper discusses the role that the milk production can carry out in a development strategy for the Rio Grande do Sul State (Brazil. It was verified that an important contribution that the milk production could provide to the development it derives of its capacity of maintenance of the population in the rural zones, which enhance its effects on the local economies. These effects are generated by the high potential of aggregation of value that the dairy activity can provide in a direct, indirect and induced way. So for the milk production can play this function, however, it is necessary orientate the current policies sustained by the representatives of the agro-industrial chain that can lead more than two thirds of the producers to the exclusion in the short period. The main conclusion of the paper is that the promotion of production systems adapted to the conditions of the family production would be an indispensable condition for the construction of a development model more equitable and balanced territorially

  17. La grande bellezza (P. Sorrentino, 2013

    Directory of Open Access Journals (Sweden)

    Daniela Brogi

    2013-05-01

    Full Text Available Qual è il soggetto della Grande bellezza? Di cosa parla il film, mentre racconta come sta al mondo Jep Gambardella? Ripartiamo dalla traccia narrativa: Jep si è trasferito a Roma da giovane, in cerca di successo – come tanti, come tutti – per occupare il centro della mondanità e diventarne imperatore, tanto da essere distolto dalla cura del proprio talento (a venticinque anni aveva conquistato la fama col suo primo e unico romanzo L’apparato umano; quarant’anni più tardi, lo guardiamo vivere: essenzialmente senza far nulla, mentre divaga continuamente, tra feste in terrazza, incontri, passaggi onirici e camminate solitarie sul Tevere; intanto giunge la notizia della morte della donna di cui era stato innamorato da ragazzo. Qual è, dunque, la storia più vera messa in scena? Quella di un’ambizione implosa? Quella dello sperpero esistenziale della mondanità capitolina? Quella di una crisi d’identità e del tentativo di ritrovare una spiritualità? Quella di Roma? 

  18. Local grand unification in the heterotic landscape

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, Jonas

    2009-07-15

    We consider the possibility that the unification of the electroweak interactions and the strong force arises from string theory, at energies significantly lower than the string scale. As a tool, an effective grand unified field theory in six dimensions is derived from an anisotropic orbifold compactification of the heterotic string. It is explicitly shown that all anomalies cancel in the model, though anomalous Abelian gauge symmetries are present locally at the boundary singularities. In the supersymmetric vacuum additional interactions arise from higher-dimensional operators. We develop methods that relate the couplings of the effective theory to the location of the vacuum, and find that unbroken discrete symmetries play an important role for the phenomenology of orbifold models. An efficient algorithm for the calculation of the superpotential to arbitrary order is developed, based on symmetry arguments. We furthermore present a correspondence between bulk fields of the orbifold model in six dimensions, and the moduli fields that arise from compactifying four internal dimensions on a manifold with non-trivial gauge background. (orig.)

  19. y las grandes ciudades: 1970-2004

    Directory of Open Access Journals (Sweden)

    Adrián de León Arias

    2008-01-01

    Full Text Available El cambio regional en las manufacturas mexicanas a partir de la década de 1980 se ha caracterizado por el rápido crecimiento del empleo y la producción en la frontera norte del país y la pérdida de participación de ellos en las grandes ciudades. En este artículo se analiza y evalúa este cambio regional en términos de la dinámica de la productividad laboral y factorial total para ambas configuraciones espaciales, presentando cálculos de productividad para éstas de 1970 a 2004, estimados a partir de la información censal. Los resultados sugieren que mientras el empleo ha crecido en magnitud mucho más rápido en la frontera norte, esta dinámica se ha generado con bajos niveles en crecimiento de la productividad, al menos hasta fechas recientes, por lo que el cambio regional estimulado por la apertura comercial hasta 2004 no parece haber facilitado un crecimiento económico más acelerado para México

  20. Toward Realistic Gauge-Higgs Grand Unification

    CERN Document Server

    Furui, Atsushi; Yamatsu, Naoki

    2016-01-01

    The $SO(11)$ gauge-Higgs grand unification in the Randall-Sundrum warped space is presented. The 4D Higgs field is identified as the zero mode of the fifth dimensional component of the gauge potentials, or as the fluctuation mode of the Aharonov-Bohm phase $\\theta_H$ along the fifth dimension. Fermions are introduced in the bulk in the spinor and vector representations of $SO(11)$. $SO(11)$ is broken to $SO(4) \\times SO(6)$ by the orbifold boundary conditions, which is broken to $SU(2)_L \\times U(1)_Y \\times SU(3)_C$ by a brane scalar. Evaluating the effective potential $V_{\\rm eff} (\\theta_H)$, we show that the electroweak symmetry is dynamically broken to $U(1)_{\\rm EM}$. The quark-lepton masses are generated by the Hosotani mechanism and brane interactions, with which the observed mass spectrum is reproduced. The proton decay is forbidden thanks to the new fermion number conservation. It is pointed out that there appear light exotic fermions. The Higgs boson mass is determined with the quark-lepton masses ...

  1. Integrated telemedicine workstation for intercontinental grand rounds

    Science.gov (United States)

    Willis, Charles E.; Leckie, Robert G.; Brink, Linda; Goeringer, Fred

    1995-04-01

    The Telemedicine Spacebridge to Moscow was a series of intercontinental sessions sponsored jointly by NASA and the Moscow Academy of Medicine. To improve the quality of medical images presented, the MDIS Project developed a workstation for acquisition, storage, and interactive display of radiology and pathology images. The workstation was based on a Macintosh IIfx platform with a laser digitizer for radiographs and video capture capability for microscope images. Images were transmitted via the Russian Lyoutch Satellite which had only a single video channel available and no high speed data channels. Two workstations were configured -- one for use at the Uniformed Services University of Health Sciences in Bethesda, MD. and the other for use at the Hospital of the Interior in Moscow, Russia. The two workstations were used may times during 16 sessions. As clinicians used the systems, we modified the original configuration to improve interactive use. This project demonstrated that numerous acquisition and output devices could be brought together in a single interactive workstation. The video images were satisfactory for remote consultation in a grand rounds format.

  2. A unified grand tour of theoretical physics

    CERN Document Server

    Griffiths, J

    2002-01-01

    Anyone offering a grand tour is faced with several options. Should they concentrate on what may be considered to be essential features, or should they attempt to present a brief glimpse of almost everything? The present offering is a compromise between these two extremes. The area considered - theoretical physics - is now such a vast subject that some kind of compromise is essential. Indeed, the field is now so wide that few could even attempt to review it in a single-authored work. My task here is to assess how well this book has succeeded in its main aim of providing a unified (though introductory) tour of this subject. Constrained within a single volume, this is clearly not an updated Landau-Lifschitz. It cannot be expected to take any particular topic to the level of recent research. Nevertheless, it does seem to cover the broad range of essential topics which now constitute the subject. It starts (most appropriately in my opinion) with geometry. It then covers classical physics, general relativity and qu...

  3. The grand descent has begun for CMS

    CERN Multimedia

    2006-01-01

    Until recently, the CMS experimental cavern looked relatively empty; its detector was assembled entirely at ground level, to be lowered underground in 15 sections. On 2 November, the first hadronic forward calorimeter led the way with a grand descent. The first section of the CMS detector (centre of photo) arriving from the vertical shaft, viewed from the cavern floor. There is something unusual about the construction of the CMS detector. Instead of being built in the experimental cavern, like all the other detectors in the LHC experiments, it was constructed at ground level. This was to allow for easy access during the assembly of the detector and to minimise the size of the excavated cavern. The slightly nerve-wracking task of lowering it safely into the cavern in separate sections came after the complete detector was successfully tested with a magnetic field at ground level. In the early morning of 2 November, the first section of the CMS detector began its eagerly awaited descent into the underground ca...

  4. FORMULASI STRATEGI PENINGKATAN PRODUKSI DOMBA CV MITRA TANI FARM, CIAMPEA, BOGOR

    Directory of Open Access Journals (Sweden)

    Amalia Sholehana

    2014-04-01

    Full Text Available ABSTRACTThis research was conducted to formulate to increase the sheep production at CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. The research was conducted using descriptive analysis by analyzing the problem experienced by CV. MitraTani Farm. Primary data and secondary data were gathered at this research. The research was also conducted using several analytical methods such as value chain mapping, internal factor evaluation (IFE and external factor evaluation (EFE matrix, SWOT and quantitative strategic planning matrix (QSPM. Based on the value chain analysis, the current production of sheep at CV MT farm is highly depending on the supply from its partners and internal farmers. According to the IFE and EFE results, the differences of each weighted values were respectively 2,120 and 0,686 so the position of the company in the SWOT matrix was situated at Quadrant I. Therefore the company needs to use a growth strategy. The QSPM sequenced the strategy priority as follow (1 increasing the number of lambs, (2 developing the management, (3 strengthening the capital and ownership, (4 improving the quality through technology. The result of the gross profit margin calculation, if the sheep‘s selling is increased up to 1000 sheep per month then the possible annual profit that can be earned by CV MT farm is 40,34% of the total production.Keywords: MT Farm, sheep production, strategies, SWOT, QSPM, gross profit marginABSTRAKPenelitian ini dilakukan untuk memformulasikan strategi peningkatan produksi domba di CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. Pendekatan penelitian yang diterapkan adalah analisis deskriptif yang dilakukan dengan mempelajari permasalahan dari objek yang diteliti. Data yang dikumpulkan dalam penelitian ini adalah data primer dan data sekunder. Penelitian dilakukan menggunakan alat analisis berupa pemetaan rantai nilai,  matriks IFE (Internal Factor Evaluation dan EFE (External Factor Evaluation, SWOT, dan QSPM (Quantitative

  5. 53Mn-53Cr chronology of Ca-Fe silicates in CV3 chondrites

    Science.gov (United States)

    MacPherson, Glenn J.; Nagashima, Kazuhide; Krot, Alexander N.; Doyle, Patricia M.; Ivanova, Marina A.

    2017-03-01

    High precision secondary ion mass-spectrometry (SIMS) analyses of kirschsteinite (CaFeSiO4) in the reduced CV3 chondrites Vigarano and Efremovka yield well resolved 53Cr excesses that correlate with 55Mn/52Cr, demonstrating in situ decay of the extinct short-lived radionuclide 53Mn. To ensure proper correction for relative sensitivities between 55Mn+ and 52Cr+ ions, we synthesized kirschsteinite doped with Mn and Cr to measure the relative sensitivity factor. The inferred initial ratio (53Mn/55Mn)0 in chondritic kirschsteinite is (3.71 ± 0.50) × 10-6. When anchored to 53Mn-53Cr relative and U-corrected 207Pb-206Pb absolute ages of the D'Orbigny angrite, this ratio corresponds to kirschsteinite formation 3.2-0.7+08 Ma after CV Ca-, Al-rich inclusions. The kirschsteinite data are consistent within error with the data for aqueously-formed fayalite from the Asuka 881317 CV3 chondrite as reported by Doyle et al. (2015), supporting the idea that Ca-Fe silicates in CV3 chondrites are cogenetic with fayalite (and magnetite) and formed during metasomatic alteration on the CV3 parent body. Concentrically-zoned crystals of kirschsteinite and hedenbergite indicate that they initially formed as near end-member compositions that became more Mg-rich with time, possibly as a result of an increase in temperature.

  6. 火龙果愈伤组织诱导与植株再生%Callus Induction and Plantlet Regeneration from Pitaya

    Institute of Scientific and Technical Information of China (English)

    黄红梅; 任希望; 刘清波

    2012-01-01

    为了建立火龙果愈伤组织诱导与植株再生体系,以火龙果茎段、幼苗和子叶为外植体进行离体培养试验.结果表明:茎段诱导愈伤组织的最优培养基为1/2MS+2,4-D 2.0 mg·L-16-BA 0.5 mg·L-1,诱导子叶愈伤组织的最适培养基是1/2MS+2,4-D 2.0 mg·L-1+6-BA 1.0 mg·L-1,诱导愈伤组织分化的最优培养基为1/2MS+6-BA 4.0 mg·L-1+NAA 0.5 mg·L-1,最佳生根培养基为1/2MS+6-BA 1 mg·L-1+NAA 0.3 mg·L-1.%To establish the system of callus induction and plantlet regeneration from pitaya,pitaya was in vitro cultured using stem segments,seedlings and cotyledons as explants. The results showed the optimum medium for the callus induction of stem segments was l/2MS+2,4-D 2.0 mg·L-1+6-BA 0.5 mg·L1. The most suitable medium to induce the callus from cotyledons was 1/2MS+2.4-D 2.0 mg·L-1+6-BA 1.0 mg·L-1. The optimal medium for inducing the callus differentiation was 1/2MS+6-BA 4.0 mg·L-1+NAA 0.5 mg·L-1. The greatest medium of root regeneration was 1/2MS+6-BA 1.0 mg·L-1+NAA0.3 mg·L-1.

  7. Grand Cote National Wildlife Refuge [Land Status Map

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This map was produced by the Division of Realty to depict landownership at Grand Cote National Wildlife Refuge. It was generated from rectified aerial photography,...

  8. Grand Bay National Wildlife Refuge: Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on Grand Bay NWR for the next 15 years. This plan outlines the Refuge vision and purpose...

  9. From Grand Narratives of Democracy to Small Expectations of Participation

    DEFF Research Database (Denmark)

    Peters, Chris; Witschge, Tamara

    2016-01-01

    This chapter critically examines the invocation of democracy in the discourse of audience participation in digital journalism. Rather than simply restate the familiar grand narratives that traditionally described journalism’s function for democracy (information source, watchdog, public representa...

  10. Casa Grande Ruins National Monument Vegetation Mapping Project - Plot Locations

    Data.gov (United States)

    National Park Service, Department of the Interior — This layer contains spatial information for 25 plots sampled during vegetation mapping and classification efforts at Casa Grande Ruins NM, AZ. Data was collected by...

  11. Grand Performances: Building Self and Social Ties through Theatrical Performance

    Directory of Open Access Journals (Sweden)

    Stephanie May de Montigny

    2015-05-01

    Full Text Available The stories that individuals tell about their experiences on stage and behind-the-scenes at the Grand Opera House of Oshkosh, Wisconsin, contribute to their knowledge of self and social ties.

  12. The KASCADE-Grande Experiment and the LOPES Project

    CERN Document Server

    Badea, A F; Apel, W D; Bekk, K; Bercuci, A; Bertaina, M; Blümer, H; Bozdog, H; Brancus, I M; Bruggemann, M; Bucholz, P; Chiavassa, A; Daumiller, K; di Pierro, F; Doll, P; Engel, R; Engler, J; Falcke, H; Fessler, F; Ghia, P L; Gils, H J; Glasstetter, R; Haungs, A; Heck, D; Hörandel, J R; Horneffer, A; Huege, T; Kampert, K H; Kant, G W; Klages, H O; Kolotaev, Yu; Maier, G; Mathes, H J; Mayer, H J; Milke, J; Morello, C; Müller, M; Navarra, G; Obenland, R; Oehlschläger, J; Ostapchenko, S; Petcu, M; Plewnia, S; Rebel, H; Risse, A; Roth, M; Schieler, H; Scholz, J; Stumpert, M; Thouw, T; Toma, G; Trinchiero, G C; Ulrich, H; Valchierotti, S; Van Buren, J; De Vos, C M; Walkowiak, W; Weindl, A; Wochele, J; Zabierowski, J; Zagromski, S; Zimmermann, D

    2004-01-01

    KASCADE-Grande is the extension of the multi-detector setup KASCADE to cover a primary cosmic ray energy range from 100 TeV to 1 EeV. The enlarged EAS experiment provides comprehensive observations of cosmic rays in the energy region around the knee. Grande is an array of 700 x 700 sqm equipped with 37 plastic scintillator stations sensitive to measure energy deposits and arrival times of air shower particles. LOPES is a small radio antenna array to operate in conjunction with KASCADE-Grande in order to calibrate the radio emission from cosmic ray air showers. Status and capabilities of the KASCADE-Grande experiment and the LOPES project are presented.

  13. Kevadise lilleseadevõistluse Grand Deco 2004 eelvoor / Piret Veigel

    Index Scriptorium Estoniae

    Veigel, Piret, 1961-

    2004-01-01

    17.-18. IV toimub Palmse mõisas rahvusvaheline lilleseade meistrivõistlus Grand Deco 2004, korraldajaks firma Tourinest. Žürii esimees Michael Bowyer Suurbritanniast. Eestit esindab žüriis Priit Press. Eelvoorus osalemisest

  14. VT Built Up Lands in Grand Isle County - 1977

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  15. VT Developed Lands in Grand Isle County - 2003

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  16. VT Built Up Lands in Grand Isle County - 1941

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  17. VT Built Up Lands in Grand Isle County - 1995

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  18. VT Built Up Lands in Grand Isle County - 1962

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  19. VT Built Up Lands in Grand Isle County - 1980

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  20. VT Built Up Lands in Grand Isle County - 1986

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  1. Albuquerque/Middle Rio Grande Urban Waters Viewer

    Data.gov (United States)

    U.S. Environmental Protection Agency — These data have been compiled in support of the Middle Rio Grande/Albuquerque Urban Waters Partnership for the region including Albuquerque, New Mexico.The Middle...

  2. VT Built Up Lands in Grand Isle County - 1974

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  3. Consumo y entretenimiento: los grandes almacenes como espacio de sociabilidad

    National Research Council Canada - National Science Library

    Rocha, Everardo; Amaral, Maria

    2009-01-01

    .... Por un lado, la aparicion de los grandes almacenes como un nuevo lugar de sociabilidad y, por el otro, este mismo lugar como parte de la construccion de la propia identidad del consumidor moderno...

  4. Grand Cote National Wildlife Refuges Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Grand Cote NWR Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern at the Refuge, to...

  5. Grand Cote National Wildlife Refuge: Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on Grand Cote NWR for the next 15 years. This plan outlines the Refuge vision and purpose...

  6. VT Built Up Lands in Grand Isle County - 2003

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) NRCS mapped historical and current-day built-up lands for Grand Isle County, VT using several vintages of aerial photography: 1941, 1962, 1974,...

  7. Enraizamento in vitro de pereira (Pyrus communis L. cv. Carrick In vitro rooting of pear tree (Pyrus communis L. cv. Carrick

    Directory of Open Access Journals (Sweden)

    Alan Cristiano Erig

    2004-02-01

    Full Text Available O objetivo do presente trabalho foi avaliar o efeito do ácido naftalenoacético (ANA e do carvão ativado no enraizamento in vitro de pereira (Pyrus comunis L. cv. Carrick. Para tanto, microestacas de pereira com aproximadamente 0,8 a 1cm de comprimento foram utilizadas como explantes. Os tratamentos constituíram-se de três concentração de ANA no meio de cultura (0; 3,2 e 6,4mM e de duas concentração de carvão ativado (0 e 1%. A partir dos resultados obtidos no experimento, conclui-se que o ANA nas concentrações de 3,2 e 6,4mM e na ausência de carvão ativado no meio de cultura, possibilitou um melhor enraizamento de pereira cv. Carrick.The aim of the present work was to evaluate the effects of naphthaleneacetic acid (NAA and activated charcoal on the in vitro rooting of the pear tree (Pyrus communis L. cv. Carrick. Therefore pear tree microshoots with approximately 0.8 to 1cm of length were used as explant. The treatments were constituted of three concentration of NAA in the culture medium (0; 3.2 and 6.4mM and two concentration of activated charcoal (0 and 1%. NAA in the concentration of 3.2 and 6.4mM and in the absence of activated charcoal in the culture medium, showed better rooting rate to pear tree cv. Carrick.

  8. Chlorophyll Fluorescence in Partially Defoliated Grape Plants (Vitis vinifera L. cv. Chardonnay) / Fluorescencia de la Clorofila en Plantas de Uva (Vitis vinifera L. cv. Chardonnay) Defoliadas Parcialmente

    OpenAIRE

    Peña Olmos Jaime Ernesto; Casierra Posada Fánor

    2013-01-01

    The chlorophyll content and fluorescence weredetermined in five-year-old grape plants (Vitis vinifera L. cv.Chardonnay) that were subjected to early partial defoliation,in Villa de Leyva, Colombia. The experimental design wascompletely randomized, consisting of two treatments (50%defoliation and control), each with four replications of 35 plants. Every two weeks, one of every two recently-emerged leaves was removed from the non-control plants. The determination of total chlorophyll content wa...

  9. 3类观赏百合试管苗叶片结构及水分特性%Comparative Study on the Structural and Moisture Characteristics of Leaf from the Plantlets of Three Types of Ornamental Lilium brownii

    Institute of Scientific and Technical Information of China (English)

    唐蓉; 龚维红; 史文秀; 韦梅芹

    2009-01-01

    [Objective] The study was to compare the structural and moisture characteristics of leaf from the plantlets of three types of ornamental lily(Lilium brownii). [Method] The paraffin sections of leaves of tested lily varieties were prepared and then observed under microscope, and the stomatal characteristics and moisture characteristics of tested lily varieties were measured. [Result] All the three ornamental lily varieties show isobilateral leaf, single layer of epicuticula and lower epidermis, and no obvious differentiation of palisade tissue and spongy tissue; their stomata distribute in lower epidermis, and the guard cells are dumbbell-shaped; all of these materials present high moisture. For the leaf sickness, midrib sickness and mesophyll tissue sickness, the order was determined to be oriental lily>Lilium longiflorum>Asian lily; of the three types of ornamental lily, Lilium longiflorum has the largest stomatal aperture and Asian lily has the smallest; focusing the water potential and moisture, the turn was Asian lily> oriental lily> Lilium longiflorum. [Conclusion] The study may facilitate the artificial regulation of the growth conditions of the plantlets of ornamental lily.

  10. Light equation in eclipsing binary CV Boo: third body candidate in elliptical orbit

    CERN Document Server

    Bogomazov, A I; Satovskii, B L; Krushevska, V N; Kuznyetsova, Yu G; Ehgamberdiev, Sh A; Karimov, R G; Khalikova, A V; Ibrahimov, M A; Irsmambetova, T R; Tutukov, A V

    2016-01-01

    A short period eclipsing binary star CV Boo is tested for the possible existence of additional bodies in the system with a help of the light equation method. We use data on the moments of minima from the literature as well as from our observations during 2014 May--July. A variation of the CV Boo's orbital period is found with a period of $\\approx 75$ d. This variation can be explained by the influence of a third star with a mass of $\\approx 0.4M_{\\odot}$ in an eccentric orbit with $e\\approx 0.9$. A possibility that the orbital period changes on long time scales is discussed. The suggested tertiary companion is near the chaotic zone around the central binary, so CV Boo represents an interesting example to test its dynamical evolution. A list of 14 minima moments of the binary obtained from our observations is presented.

  11. Environmental Assessment: Demolish 452 at Grand Forks Air Force Base

    Science.gov (United States)

    2005-12-01

    Preserve” has been developed to restore a part of the native tallgrass prairie that once was dominant in this region. Plants thriving in this...native wildflower species. The Grand Forks AFB Natural Resources Manager installed a butterfly garden in the Prairie View Nature 32 Preserve in the...characterized as typical prairie potholes found within the northern plains ecoregion. 31 Wetlands on Grand Forks AFB occur frequently in drainage

  12. South African Formula One Grand Prix: a dream or nightmare.

    OpenAIRE

    2008-01-01

    In 2004 a group of South African based companies submitted a bid to the Formula One Management to host a South African Formula One Grand Prix, from 2009 in Cape Town. The group approached the government to establish a public-private partnership, due to the: • escalating hosting and infrastruture development cost, resulting in very few Formula One Grand Prix host countries undertaking hosting without government involvement; and • believe that the economic benefits of hosting the event would st...

  13. The canonical and grand canonical models for nuclear multifragmentation

    Indian Academy of Sciences (India)

    G Chaudhuri; S Das Gupta

    2010-08-01

    Many observables seen in intermediate energy heavy-ion collisions can be explained on the basis of statistical equilibrium. Calculations based on statistical equilibrium can be implemented in microcanonical ensemble, canonical ensemble or grand canonical ensemble. This paper deals with calculations with canonical and grand canonical ensembles. A recursive relation developed recently allows calculations with arbitrary precision for many nuclear problems. Calculations are done to study the nature of phase transition in nuclear matter.

  14. Simulations of Precipitation Variability over the Upper Rio Grande Basin

    Energy Technology Data Exchange (ETDEWEB)

    Costigan, Keeley R.; Bossert, James E.; Langley, David L.

    1997-12-31

    In this research, we study Albuquerque`s water and how it may be affected by changes in the regional climate, as manifested by variations in Rio Grande water levels. To do this, we rely on the use of coupled atmospheric, runoff, and ground water models. Preliminary work on the project has focused on uncoupled simulations of the aquifer beneath Albuquerque and winter precipitation simulations of the upper Rio Grande Basin. The latter is discussed in this paper.

  15. Grand Bay-Banks Lake Stewardship Partnership - Phase 2

    Science.gov (United States)

    2006-11-01

    depending upon the drop from the Shiner Pond dike at full pool to the bottom of the original natural drain (not the swamp floor ) below. No mill...county would be appropriate. Similar soils at Craig’s Pond are mapped as: 1) Ogeechee - fine-loamy, siliceous, thermic Typic Ochraquults 2) Rembert...clayey, kaolinitic, thermic Typic Ochraquults These two soils have clayey textures very similar to those on the bottom of Grand Bay Grand Bay

  16. Tres grandes plataformas marinas Mar del Norte

    Directory of Open Access Journals (Sweden)

    Soulas, R.

    1978-03-01

    Full Text Available This article analyzes the different building stages of three large sea platforms. After studying the structure's main characteristics, preparing scale models and calculating ail the elements, platform construction was carried out in three stages: — Dry-dock construction of the hull base. — Installation in the water and finishing the structure. — Immersion and bridge installation. Finally, the necessary operations to move the platform to its definite location were carried out. The structures are formed by a parallelepiped hull of reinforced concrete, divided into compartments by means of vertical orthogonals panels on top of which 2 or 4 piles are placed to support the bridge.

    En este artículo se analizan las diversas etapas en la realización de tres grandes plataformas marinas. Después de un estudio de las características principales de la estructura, ensayos en modelo reducido y cálculo de todos los elementos, se procede a la construcción de las plataformas en tres fases: — Construcción en seco de la base del casco. — Colocación en el agua y acabado de la estructura. — Inmersión y colocación del puente. Por último, se realizan las operaciones necesarias para llevar la plataforma a su ubicación definitiva. Las estructuras están formadas por un casco paralelepipédico de hormigón armado, dividido en compartimientos mediante tabiques verticales ortogonales y sobre el que se han colocado 2 ó 4 pilas que soportan el puente.

  17. The Grand Banks Landslide area revisited

    Science.gov (United States)

    Krastel, S.; Feldens, P.; Georgiopoulou, A.; Lindhorst, K.; Llopart, J.; Mehringer, L.; Mosher, D. C.; Piper, D.; Roskoden, R.; Schoenke, M.; Schulten, I.; Stevenson, C.

    2016-12-01

    On November 18, 1929, a M7.2 earthquake occurred beneath the Laurentian Channel off the coast of Newfoundland. Nearly simultaneously, 12 undersea trans-Atlantic communication cables were severed and within two hours, a devastating tsunami struck the south coast of Newfoundland, claiming 28 lives. Only in 1952, it was understood that a slump-generated turbidity current caused the sequential severance of the cables and likely generated the tsunami. The landslide site was visited numerous times as underwater survey technologies evolved. No major head scarp related to the event is recognized. The landslide appears to have affected shallow sediments (top 5-50 m) and was laterally extensive. In order to test the hypothesis that a distributed, laterally extensive, shallow submarine mass failure event caused the tsunami, we collected 1500 km of seismic lines in combination with a dense net of hydroacoustic data during RV Maria S. Merian cruise in autumn 2015. Coring and CPT transects were carried out based on the acoustic data. The data in the failure area show abundant small scarps and several young landslide deposits. Listric faults in the failure area may play an important role for tsunami generation but need to be investigated further. The turbidity currents related to the failure entered the Grand Banks channel system. A complex pattern of bypassing and deposition is found in this complex channel area downslope of the failure area. Very coarse gravel was sampled up to 150 m above the canyon thalweg. First estimates suggest high concentrations of sediments in the flow, which was able to run out over 1000s of kilometers. We have not identified indications for similar large events in the late Holocene.

  18. Observational Needs from the WCRP Grand Challenges

    Science.gov (United States)

    Carlson, David

    2016-07-01

    An innovative history of platforms and instruments has provided remarkable satellite-based views of and data about our planet over roughly the past 35 years. The fragile and temporary nature of these satellite systems remind us that, from a climate viewpoint, we have not in any way solved or completed our observational tasks. Instead, the challenges of using satellites to observe key features of our planet's climate grow more complicated and the need grows more urgent. Admitting that we have borrowed and adopted some of our current capabilities from meteorological satellites, we need continuity of what we have as well as innovation and extension to reach new features that we now recognise as important. The scientific goals of the WCRP Grand Challenges emphasise this simultaneous need for continuation and innovation. We need longer better records of global changes in cloud types and distributions but we also need sharp accurate views of water vapour beneath those clouds. We need gravity missions to continue to track the net mass losses from Greenland and Antarctica while at the same time we need innovative remote sensing techniques to quantify rapid ice discharges at their peripheries. We need longer and broader latitudinal records of precipitation, snow cover and soil moisture but we also crucially need to detect hydrological and biogeochemical processes at the root zone. We must maintain continuity and consistency of ocean surface temperature, surface salinity and surface wind records while we also develop new methods to reveal and record deep ocean temperatures and currents. For immediate scientific, economic and political reasons we must develop a comprehensive CO2 monitoring system. And we need open high-quality access to all these data to understand present and preceding conditions as we encounter the surprises of the Anthropocene.

  19. The 1929 Grand Banks submarine landslide revisited

    Science.gov (United States)

    Schulten, Irena; Mosher, David C.; Krastel, Sebastian; Piper, David J. W.; Kienast, Markus

    2017-04-01

    On November 18th, 1929 a large submarine landslide occurred along the St. Pierre Slope of the southwestern Grand Banks of Newfoundland, as a result of a Mw 7.2 earthquake. This submarine landslide led to the first recognition of naturally-occurring submarine turbidity currents and is one of the few landslides known to have generated a tsunami. The event caused 28 causalities in Newfoundland and severe infrastructural damage. Earlier investigations of the area identified widely distributed shallow mass failures (15 - 20 m high escarpments), but no evidence of a larger headscarp. It is difficult to conceive, therefore, how this distributed shallow failure that rapidly evolved into a turbidity current would have generated a tsunami. It is hypothesised in this study that a deeper rooted sediment failure ( 500 m), involving faulting and mass-rotation, was involved in the sediment failure and this displacement generated the tsunami. In order to test this hypothesis, the volume and kinematics of the 1929 slope failure are analysed by means of recently acquired high resolution seismic reflection and multibeam swath bathymetry data, in addition to a significant volume of legacy data. The data allow determination of: 1) the dimension of the failure area, 2) the thickness and volume of failed sediment on St. Pierre Slope, 3) fault patterns and displacements, and 4) styles of sediment failure involved. Shallow (20 m high) sinuous escarpments and a number of faults are observed along the upper St. Pierre Slope (500 - 2 500 m water depth). The uppermost and largest of these escarpments shows association with a fault system. Preliminary results, therefore, indicate a complex sediment failure pattern along the St. Pierre Slope, possibly involving a deep-seated decollement and mobilization of a large volume of surficial sediment through retrogressive failure. Causes for the tsunami are yet to be determined.

  20. A COMPARATIVE STUDY TO ASSES THE OUTCOME OF GRAND MULTIPARA WITH THAT OF GREAT GRAND MULTIPARA

    Directory of Open Access Journals (Sweden)

    Nayana

    2015-01-01

    Full Text Available NEED FOR THE STUDY: In a developing country like India , where poverty , illiteracy , ignorance and lack of knowledge of family planning facilities available have greatly increased the incidence of grandmultipara 2. Grandmultipara has been associated with increased maternal morbidity and mortality rat es as well as poor outcome. The investigator is interested in finding out the outcome in grand multipara women and great grand multipara 1 women during the antenatal , internatal and postnatal period , and also to analyze demographic characteristics such as socioeconomic status and religion of the sample. STATEMENT OF THE PROBLEM: A comparative study to assess the outcome of grandmultipara (1 with that of grandmultipara women. OBJECTIVE OF THE STUDY: 1. To findout the outcome of grandmultipara women during th e antenatal , internatal and postnatal period. 2. To find the outcome of great grandmultipara women during the antenatal , internatal (2 and postnatal period. 3. To compare the outcomes of grandmultipara with that of non - grandmultipara women during the anten atal , internatal and postnatal (3 period. ( 4 To analyze the demographic characteristics 6 of the sample i.e. income and religion.

  1. Fauna reptiliana do norte da grande Porto Alegre, Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Thales de Lema

    1983-01-01

    Full Text Available This paper reports the preliminary results of two years of herpetogeographic studies in the region called «Grande Porto Alegre» at its northern portion. The physiognomy shows, at North, slopes of the Brazilian Southern Plateau, more or less forested in a general view; these elevations graãuálly decrease to the South until the levei of the sea to Atlantic littoral by the eastern border, and to Rio Grande do Sul savanah, which extends to Uruguay and Argentina. The biota is transitional from the highland prairies and forests of the plateau to lowland prairies of the pampas. Three faunistic Provinces are included: Guarani, Pampean, and Tupi. The most conspicuous dispersion is shown by the taxa proceeding from the plateau to savanah (Dispersion Centre Guarani; the pampean species occur in low percentage (Dispersion Centre Uruguay, and an inappreciable account of Tupi fauna (Dispersion Centre of «Serra do Mar». The new records are: presence of Chironius pyrrhopogon, Hydrodynastes gigas, Bothrops neuwiedi para-naensis, Micrurus corallinus; intergradation zone of Guarani and Pampean geographic roces; and new taxa of snakes, which will be described brieflly. The systematic composition presents: Chelonia, 4 (3 Chelidae, 1 Testudinidae; Sauria, 10 (1 Gekkonidae, 2 Iguanidae, 1 Anguidae, 3 Teiidae, 3 Amphisbaenidae; Serpentes, 45 (35 Colubridae, 4 Elapidae, 6 Viperidae; Crocodylia (1 Crocodylidae.

  2. Khatyrka, a new CV3 find from the Koryak Mountains, Eastern Russia

    Science.gov (United States)

    MacPherson, Glenn J.; Andronicos, Christopher L.; Bindi, Luca; Distler, Vadim V.; Eddy, Michael P.; Eiler, John M.; Guan, Yunbin; Hollister, Lincoln S.; Kostin, Alexander; Kryachko, Valery; Steinhardt, William M.; Yudovskaya, Marina; Steinhardt, Paul J.

    2013-08-01

    A new meteorite find, named Khatyrka, was recovered from eastern Siberia as a result of a search for naturally occurring quasicrystals. The meteorite occurs as clastic grains within postglacial clay-rich layers along the banks of a small stream in the Koryak Mountains, Chukotka Autonomous Okrug of far eastern Russia. Some of the grains are clearly chondritic and contain Type IA porphyritic olivine chondrules enclosed in matrices that have the characteristic platy olivine texture, matrix olivine composition, and mineralogy (olivine, pentlandite, nickel-rich iron-nickel metal, nepheline, and calcic pyroxene [diopside-hedenbergite solid solution]) of oxidized-subgroup CV3 chondrites. A few grains are fine-grained spinel-rich calcium-aluminum-rich inclusions with mineral oxygen isotopic compositions again typical of such objects in CV3 chondrites. The chondritic and CAI grains contain small fragments of metallic copper-aluminum-iron alloys that include the quasicrystalline phase icosahedrite. One grain is an achondritic intergrowth of Cu-Al metal alloys and forsteritic olivine ± diopsidic pyroxene, both of which have meteoritic (CV3-like) oxygen isotopic compositions. Finally, some grains consist almost entirely of metallic alloys of aluminum + copper ± iron. The Cu-Al-Fe metal alloys and the alloy-bearing achondrite clast are interpreted to be an accretionary component of what otherwise is a fairly normal CV3 (oxidized) chondrite. This association of CV3 chondritic grains with metallic copper-aluminum alloys makes Khatyrka a unique meteorite, perhaps best described as a complex CV3 (ox) breccia.

  3. A Control Technology for Plantlets Fast Growing of Rhododendron chrysanthum Pall.in Changbai Mountain%长白山牛皮杜鹃种苗速生调控技术

    Institute of Scientific and Technical Information of China (English)

    顾地周; 沈红梅; 闫中雪; 徐鹏佳; 袁浩; 杨丽娟; 王秋爽; 张学士

    2014-01-01

    We used the plantlets of Rhododendron chrysanthum Pall.to study the main factors with different levels by repeated experiments with multi factorial for establishing a control system of plantlets fast growing of R.chrysanthum.The the main factors with levels for plantlets fast growing of R.chrysanthum are under the light hours of 13 h· d-1 , the interval time of topdressing nutrient solution of 21-22 days, the light intensity of 700-800 lx and the temperature of 26℃.The longitudi-nal growth height and transverse diameter of plantlets of R.chrysanthum were 17.6 cm and 6.0 mm in 25 d, and 26.0 cm and 7.4 mm in 50 d, respectively.The growing height and stem diameter of contrast plant were only 8.8 cm和2.0 mm in 25 d, and 10.2 cm and 2.9 mm in 50 d, respectively.We got the optimization for plantlets fast growing of R.chrysanthum.%本研究以牛皮杜鹃组培苗为供试植物,通过多因子交叉重复试验对影响牛皮杜鹃组培苗快速生长的各主要因素及其水平的作用进行了探讨,旨在探索并建立一种牛皮杜鹃种苗快速生长的调控方法。结果表明:最适合牛皮杜鹃组培苗快速生长的主要因素及其水平是光照时间为13 h· d-1、营养液追施间隔时间为21~22 d、光照强度为700~800 lx和温度为26℃。经过25 d的调控处理,牛皮杜鹃组培苗纵向生长高度和横向直径分别达到17.6 cm和6.0 mm,对照组仅分别达到8.8 cm和2.0 mm;50 d后纵向生长高度和横向直径分别达到26.0 cm和7.4 mm,而对照组仅分别达到10.2 cm和2.9 mm。初步得到牛皮杜鹃种苗速生的优化组合,该组合将有利于牛皮杜鹃种苗的快速生长。

  4. Resposta de plantas micropropagadas de abacaxizeiro à inoculação de bactérias diazotróficas em casa de vegetação Response of pineapple plantlets to inoculation with diazotrophic bacteria in greenhouse

    Directory of Open Access Journals (Sweden)

    Olmar Baller Weber

    2003-12-01

    Full Text Available O objetivo deste trabalho foi selecionar e avaliar bactérias diazotróficas isoladas de abacaxizeiro (Ananas comosus (L. Merril no desenvolvimento de cultivares micropropagadas da mesma espécie em casa de vegetação. Plantas da cultivar Perolera foram submetidas à inoculação com Asaia bogorensis (AB219 e cultivadas em tubetes, durante 145 dias, com as misturas: casca de arroz carbonizada, folha de carnaubeira triturada e vermicomposto; casca de arroz carbonizada, pó da casca do coco maduro e vermicomposto; casca de arroz carbonizada, vermiculita e vermicomposto. Plantas da cultivar Primavera receberam inóculos com o AB219 e bactérias relacionadas a Burkholderia cepacia (AB202 e AB213, enquanto plantas das cultivares Pérola e Smooth Cayenne receberam AB219 e AB213, sendo cultivadas, por 140 dias, em tubetes com a mistura de vermicomposto e vermiculita. A colonização dos abacaxizeiros pelas bactérias diazotróficas foi confirmada. As plantas da cultivar Perolera cresceram melhor em casca de arroz carbonizada, vermiculita e vermicomposto e responderam positivamente ao AB219. Já as plantas da cultivar Primavera não apresentaram resposta significativa à inoculação com AB219, AB202 e AB213. Houve incremento de 23,1% a 38,5% na matéria seca de raízes das plantas da cultivar Pérola na presença de AB213 e AB219, respectivamente. A presença de AB213 incrementou em 15,2% a matéria seca da parte aérea das plantas da cultivar Smooth Cayenne. Os resultados revelam a eficiência de bactérias diazotróficas na promoção do crescimento de abacaxizeiros.The objective of this work was to select and to evaluate isolates of diazotrophic bacteria associated to pineapple (Ananas comosus (L. Merril plants in the development of micropropagated plantlets of cultivars pineapple in greenhouse. Plantlets from the Perolera cultivar have been submitted to inoculation with Asaia bogorensis (AB219 and were cultivated during 145 days on the following

  5. Auditory preattentive processing of Thai vowel change perception in consonant-vowel (CV syllables

    Directory of Open Access Journals (Sweden)

    Naiphinich Kotchabhakdi

    2004-11-01

    Full Text Available Event-related potential (ERP responses to infrequently presented spoken deviant syllables /pi/ among repetitive standard /pc/ syllables were recorded in Thai subjects who ignored these stimuli while reading books of their choices. The vowel across-category changes elicited a change-specific mismatch negativity response (MMN. The across-category change perception of vowels in consonant-vowel (CV syllables was also assessed using low- resolution electromagnetic tomography (LORETA. The LORETA-MMN generator appeared in the left auditory cortex, emphasizing the role of the left hemisphere in speech processing already at a preattentive processing level also in CV-syllables.

  6. Study of Antimicrobial and Cytotoxic Potential of the Oyster Mushroom Pleurotus ostreatus cv. Florida (Agaricomycetes).

    Science.gov (United States)

    Ganeshpurkar, Aditya; Bhadoriya, Santosh Singh; Pardhi, Priya; Jain, Alok Pal; Rai, Gopal

    2016-01-01

    This work was undertaken to evaluate in vitro antimicrobial and cytotoxic potential of Pleurotus ostreatus cv. Florida. Mushroom basidiocarps were extracted in water:ethanol (1:1, v/v), and the resulting extract was subjected to antimicrobial studies against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella oxytoca, Bacillus subtilis, and Candida albicans. Cytotoxic potential on viable human leukocytes was studied. In vitro results showed excellent antimicrobial and cytotoxic potentials of the mushroom extract. Thus, functional properties of P. ostreatus cv. Florida could be used in the search for novel therapeutics.

  7. Python y OpenCV aplicados a un caso de estudio real

    OpenAIRE

    García del Arco, José Antonio

    2015-01-01

    Este trabajo se centra en el uso del lenguaje Python y la librería OpenCV de visión por computador para el seguimiento de crustáceos marinos en condiciones experimentales y determinar su comportamiento en un entorno social. Aquest treball es centra en l'ús del llenguatge Python i la llibreria OpenCV de visió per computador per al seguiment de crustacis marins en condicions experimentals i determinar el seu comportament en un entorn social.

  8. 76 FR 28687 - Proposed Amendment of Class E Airspace; Grand Marais, MN

    Science.gov (United States)

    2011-05-18

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Grand Marais, MN...: This action proposes to amend Class E airspace at Grand Marais, MN. Additional controlled airspace is... procedures at Grand Marais/Cook County Airport, Grand Marais, MN. Controlled airspace is needed for the...

  9. Enterotoxemia em caprinos no Rio Grande do Sul Caprine enterotoxaemia in Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Edson M. Colodel

    2003-12-01

    Full Text Available São descritos surtos de enterotoxemia em caprinos em cinco propriedades no Estado do Rio Grande do Sul. Os animais afetados eram, normalmente, encontrados mortos ou apresentavam evolução aguda de 2 a 3 horas com acentuada depressão, cólicas abdominais e diarréia profusa com fibrina. Em duas propriedades relataram-se casos com a evolução de até 12 horas. Em treze animais necropsiados observaram-se aumento de líquidos nas cavidades abdominal, torácica e pericárdica, congestão e hiperemia da serosa e mucosa do intestino, conteúdo do cólon líquido com fibrina além de hemorragias de serosa e fibrina. Em um animal constatou-se microangiopatia cerebral caracterizada por acúmulo de material homogêneo e eosinofílico no espaço perivascular. No conteúdo intestinal, colônias com bastonetes morfológica e bioquimicamente sugestivos de Clostridium perfringens foram caracterizadas no estudo bacteriológico. A soroneutralização em camundongos com conteúdo intestinal dos animais afetados, revelou a presença da toxina épsilon. Estes achados evidenciam a enterotoxemia como doença de importância para criação de caprinos no Rio Grande do Sul.Five outbreaks of caprine enterotoxaemia in Rio Grande do Sul, Brazil, were studied. The animals were found dead or had a clinical course that usually lasted 2-3 hours. From two farms a clinical manifestation period of 12 hours was reported. Clinical signs were characterized by depression, marked abdominal discomfort, profuse watery diarrhea with fibrin clots, and death. Thirteen necropsies were performed and hydropericardium, hydrothorax and hydroperitoneum were commonly found. The mucosa and serosa of the colon were congested, and its contents was watery with multiple fibrin clots. Serosal hemorrhages and fibrin clots in the gallbladder were also seen. Fibrinous colitis and thyphilitis were the most frequent histological changes. Cerebral microangiopathy was observed in one case, which was

  10. Cassini's Grand Finale: The Final Orbits

    Science.gov (United States)

    Spilker, Linda; Edgington, Scott

    2016-04-01

    The Cassini-Huygens mission, a joint collaboration between NASA, ESA and the Italian Space Agency, is approaching its last year of operations after nearly 12 years in orbit around Saturn. Cassini will send back its final bits of unique data on September 15th, 2017 as it plunges into Saturn's atmosphere, vaporizing and satisfying planetary protection requirements. Before that time Cassini will continue its legacy of exploration and discovery with 12 close flybys of Titan in 2016 and 2017 that will return new science data as well as sculpt the inclinations and periods of the final orbits. Even though all of our close icy satellite flybys, including those of Enceladus, are now completed, numerous Voyager-class flybys (summer solstice approaches. In November 2016 Cassini will transition to a series of orbits with peripases just outside Saturn's F ring. These 20 orbits will include close flybys of some tiny ring moons and excellent views of the F ring and outer A ring. The 126th and final close flyby of Titan will propel Cassini across Saturn's main rings and into its final orbits. Cassini's Grand Finale, starting in April 2017, is comprised of 22 orbits at an inclination of 63 degrees. Cassini will repeatedly dive between the innermost rings and the upper atmosphere of the planet providing insights into fundamental questions unattainable during the rest of the mission. Cassini will be the first spacecraft to explore this region. These close orbits provide the highest resolution observations of both the rings and Saturn, and direct in situ sampling of the ring particles, composition, plasma, Saturn's exosphere and the innermost radiation belts. Saturn's gravitational field will be measured to unprecedented accuracy, providing information on the interior structure of the planet, winds in the outer layers of Saturn's atmosphere, and the mass distribution in the rings. Probing the magnetic field will give insight into the nature of the magnetic dynamo, telling us: why the

  11. Cassini's Grand Finale and Recent Science Highlights

    Science.gov (United States)

    Spilker, Linda J.

    2017-06-01

    After almost 13 years in Saturn orbit, the Cassini-Huygens mission has entered its final year of data collection. Cassini will return its final bits of unique data on 15 September 2017 as it plunges into Saturn’s atmosphere, vaporizing and satisfying planetary protection requirements.Since early 2016 Cassini’s orbital inclination was slowly increased towards its final inclination. In November Cassini transitioned to a series of 20 orbits with periapses just outside Saturn's F ring that included some of the closest flybys of the tiny ring moons and excellent views of the F ring and outer A ring.Cassini's final close flyby of Titan in April 2017 propelled it across Saturn’s main rings and into its final orbits. Cassini's Grand Finale began in April 2017 and is comprised of 22 orbits at an inclination of 63 degrees. Cassini is repeatedly diving between the innermost ring and Saturn's upper atmosphere providing insights into fundamental questions unattainable during the rest of the mission. It is the first spacecraft to explore this region.These close orbits provide the highest resolution observations of both the rings and Saturn, and direct in situ sampling of the ring particles' composition, plasma, Saturn's exosphere and the innermost radiation belts. Saturn's gravitational field will be measured to unprecedented accuracy, providing information on Saturn's interior structure and mass distribution in the rings. Probing the magnetic field will give insight into the nature of the magnetic dynamo and the true rotation rate of Saturn's interior. The ion and neutral mass spectrometer will sniff the exosphere and upper atmosphere and examine water-based molecules originating from the rings. The cosmic dust analyzer will sample particle composition from different parts of the main rings.Recent science highlights and science objectives from Cassini’s final orbits will be discussed.This work was carried out in part at the Jet Propulsion Laboratory, California

  12. 3D View of Grand Canyon, Arizona

    Science.gov (United States)

    2000-01-01

    The Grand Canyon is one of North America's most spectacular geologic features. Carved primarily by the Colorado River over the past six million years, the canyon sports vertical drops of 5,000 feet and spans a 445-kilometer-long stretch of Arizona desert. The strata along the steep walls of the canyon form a record of geologic time from the Paleozoic Era (250 million years ago) to the Precambrian (1.7 billion years ago).The above view was acquired by the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) instrument aboard the Terra spacecraft. Visible and near infrared data were combined to form an image that simulates the natural colors of water and vegetation. Rock colors, however, are not accurate. The image data were combined with elevation data to produce this perspective view, with no vertical exaggeration, looking from above the South Rim up Bright Angel Canyon towards the North Rim. The light lines on the plateau at lower right are the roads around the Canyon View Information Plaza. The Bright Angel Trail, which reaches the Colorado in 11.3 kilometers, can be seen dropping into the canyon over Plateau Point at bottom center. The blue and black areas on the North Rim indicate a forest fire that was smoldering as the data were acquired on May 12, 2000.Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) is one of five Earth-observing instruments launched December 18, 1999, on NASA's Terra satellite. The instrument was built by Japan's Ministry of International Trade and Industry. A joint U.S./Japan science team is responsible for validation and calibration of the instrument and the data products. Dr. Anne Kahle at NASA's Jet Propulsion Laboratory, Pasadena, Calif., is the U.S. Science team leader; Moshe Pniel of JPL is the project manager. ASTER is the only high resolution imaging sensor on Terra. The primary goal of the ASTER mission is to obtain high-resolution image data in 14 channels over the entire land surface, as

  13. Chemical Contamination of the Lower Rio Grande near Laredo, TX

    Science.gov (United States)

    Flores, B.; Ren, J.; Krishnamurthy, S.; Belzer, W.

    2006-12-01

    The Rio Grande River stretches over 2000 miles from the southern Rocky Mountains in Colorado to the tip of Texas where the Rio Grande meets the Gulf of Mexico. It is the natural boundary between U.S. and Mexico from El Paso, TX, to Brownsville, TX. The communities along the border heavily rely upon the Rio Grande as a primary source of water for consumption, agricultural uses, supporting wildlife and recreation. For many years the Rio Grande has been polluted with municipal, industrial, agricultural and farming contaminants from both sides of the border. This pollution has led to the extinction or reduction of certain wildlife species as well as affecting the health of the residences along the border. Even though great strides have been made in monitoring the Rio Grande, there has been a lack of intense monitoring data collection for pollutants such as pesticides. Three sampling sites including Manadas Creek, the Rio Grande River at International Bridge I, and USGS monitoring site 08459200 off of Highway 83 were chosen. The water quality parameters focused include temperature, pH, conductivity, dissolve oxygen (DO), salinity, total dissolved solids, nutrients, metals and pesticides. Preliminary results have shown elevated concentration of total phosphorus and ortho-phosphorus in the Manadas Creek site. Organochlorinated pesticides such as heptachlor and 4, 4 DDE were detected at various concentrations at all sites and endrin aldehyde was found at Manadas Creek site. This research has provided more information on the current chemical contamination level of the Rio Grande in the Laredo area.

  14. Coral associations of the Pleistocene Ironshore Formation, Grand Cayman

    Science.gov (United States)

    Hunter, I. G.; Jones, B.

    1996-11-01

    The 125-ka sea level, which was approximately 6 m above present-day sea level, led to the partial flooding of many Caribbean islands. On Grand. Cayman, this event led to the formation of the large Ironshore Lagoon that covered most of the western half of the island and numerous, small embayments along the south, east, and north coasts. At that time, at least 33 coral species grew in waters around Grand Cayman. This fauna, like the modern coral fauna of Grand Cayman, was dominated by Montastrea annularis, Porites porites, Acropora polmata, and A. cervicornis. Scolymia cubensis and Mycetophyllia ferox, not previously identified from the Late Pleistocene, are found in the Pleistocene patch reefs. Madracis mirabilis, Colpophyllia breviserialis, Agaricia tenuifolia, A. lamarcki, A. undata, Millepora spp., Mycetophyllia reesi, M. aliciae, and M. danaana, found on modern reefs, have not been identified from the Late Pleistocene reefs. Conversely, Pocillopora sp. cf. P. palmata, which is found in Late Pleistocene reefs, is absent on the modern reefs around Grand Cayman. The corals in the Ironshore Formation of Grand Cayman have been divided into 10 associations according to their dominant species, overall composition, and faunal diversity. Many of these associations are similar to the modern associations around Grand Cayman. Each of the Pleistocene coral associations, which can be accurately located on the known Late Pleistocene paleogeography of Grand Cayman, developed in distinct environmental settings. Overall trends identified in the modern settings are also apparent in the Late Pleistocene faunas. Thus, the diversity of the coral faunas increased from the interior of the Ironshore Lagoon to the reef crest. Similarly, the coral diversity in the Pleistocene patch reefs was related to the size of the reefs and their position relative to breaks in the barrier reef. The barrier reef included corals that are incapable of sediment rejection; whereas the patch reefs lacked

  15. Optimized growth and plant regeneration for callus of Lilium longiflorum cv. Nellie White

    Science.gov (United States)

    The rates of growth and regeneration were compared for compact callus, friable callus, and suspension cells of Lilium longiflorum cv. Nellie White to determine the optimal culture conditions. The fresh weight was higher for compact callus induced from bulb scales cultured on Murashige and Skoog’s m...

  16. Effect of LEDs on flower bud induction in Chrysanthemum morifolium cv. Zembla

    NARCIS (Netherlands)

    Singh, M.; Ieperen, van W.; Heuvelink, E.

    2013-01-01

    The effect of LEDs was studied to induce flower under artificial long days (LD) in Chrysanthemum morifolium cv. Zembla plants, using light emitting diodes (LED) @ PAR m-2 s-1 80% Red / 20% Blue maintained @ 100 µ mol m- 2 s- 1 using royal blue light @ 455 nm and red light @ 640 nm wavelengths and

  17. Endogenous auxin regulates the sensitivity of Dendrobium (cv. Miss Teen) flower pedicel abscission to ethylene

    NARCIS (Netherlands)

    Rungruchkanont, K.; Ketsa, S.; Chatchawankanphanich, O.; Doorn, van W.G.

    2007-01-01

    Dendrobium flower buds and flowers have an abscission zone at the base of the pedicel (flower stalk). Ethylene treatment of cv. Miss Teen inflorescences induced high rates of abscission in flower buds but did not affect abscission once the flowers had opened. It is not known if auxin is a regulator

  18. Study of Catalytic Reaction at Electrode-Electrolyte Interfaces by a CV-XAFS Method

    Science.gov (United States)

    Kusano, Shogo; Matsumura, Daiju; Asazawa, Koichiro; Kishi, Hirofumi; Sakamoto, Tomokazu; Yamaguchi, Susumu; Tanaka, Hirohisa; Mizuki, Jun'ichiro

    2017-01-01

    A method combining cyclic voltammetry (CV) with x-ray absorption fine structure (XAFS) spectroscopy, viz. CV-XAFS, has been developed to enable in situ real-time investigation of atomic and electronic structures related to electrochemical reactions. We use this method to study the reaction of a Pt/C cathode catalyst in the oxygen reduction reaction (ORR) in an alkaline electrolyte, using x-ray energies near the Pt LIII edge for XAFS measurements. It was found that the current induced by the ORR was first observed at approximately 0.08 V versus Hg/HgO, although the Pt valence, which is reflected in the oxidation states, remained almost unchanged. The electronic structure of the catalytic surface in the ORR was observed to be different in the negative and positive scan directions of CV measurements. Hydrogen adsorption is also discussed on the basis of the observation of this spectral change. We have demonstrated that CV-XAFS provides dynamical structural and electronic information related to electrochemical reactions and can be used for in situ real-time measurements of a catalyst.

  19. Galaxy and Mass Assembly (GAMA): The GAMA Galaxy Group Catalogue (G3Cv1)

    CERN Document Server

    Robotham, A S G; Driver, S P; Baldry, I K; Bamford, S P; Hopkins, A M; Liske, J; Loveday, J; Merson, A; Peacock, J A; Brough, S; Cameron, E; Conselice, C J; Croom, S M; Frenk, C S; Gunawardhana, M; Hill, D T; Jones, D H; Kelvin, L S; Kuijken, K; Nichol, R C; Parkinson, H R; Pimbblet, K A; Phillipps, S; Popescu, C C; Prescott, M; Sharp, R G; Sutherland, W J; Taylor, E N; Thomas, D; Tuffs, R J; van Kampen, E; Wijesinghe, D

    2011-01-01

    Using the complete GAMA-I survey covering ~142 sq. deg. to r=19.4, of which ~47 sq. deg. is to r=19.8, we create the GAMA-I galaxy group catalogue (G3Cv1), generated using a friends-of-friends (FoF) based grouping algorithm. Our algorithm has been tested extensively on one family of mock GAMA lightcones, constructed from Lambda-CDM N-body simulations populated with semi-analytic galaxies. Recovered group properties are robust to the effects of interlopers and are median unbiased in the most important respects. G3Cv1 contains 14,388 galaxy groups (with multiplicity >= 2$), including 44,186 galaxies out of a possible 110,192 galaxies, implying ~40% of all galaxies are assigned to a group. The similarities of the mock group catalogues and G3Cv1 are multiple: global characteristics are in general well recovered. However, we do find a noticeable deficit in the number of high multiplicity groups in GAMA compared to the mocks. Additionally, despite exceptionally good local spatial completeness, G3Cv1 contains signif...

  20. Endogenous auxin regulates the sensitivity of Dendrobium (cv. Miss Teen) flower pedicel abscission to ethylene

    NARCIS (Netherlands)

    Rungruchkanont, K.; Ketsa, S.; Chatchawankanphanich, O.; Doorn, van W.G.

    2007-01-01

    Dendrobium flower buds and flowers have an abscission zone at the base of the pedicel (flower stalk). Ethylene treatment of cv. Miss Teen inflorescences induced high rates of abscission in flower buds but did not affect abscission once the flowers had opened. It is not known if auxin is a regulator

  1. Automatic tracking of red blood cells in micro channels using OpenCV

    Science.gov (United States)

    Rodrigues, Vânia; Rodrigues, Pedro J.; Pereira, Ana I.; Lima, Rui

    2013-10-01

    The present study aims to developan automatic method able to track red blood cells (RBCs) trajectories flowing through a microchannel using the Open Source Computer Vision (OpenCV). The developed method is based on optical flux calculation assisted by the maximization of the template-matching product. The experimental results show a good functional performance of this method.

  2. Cherry Picking Robot Vision Recognition System Based on OpenCV

    Directory of Open Access Journals (Sweden)

    Zhang Qi Rong

    2016-01-01

    Full Text Available Through OpenCV function, the cherry in a natural environment image after image preprocessing, color recognition, threshold segmentation, morphological filtering, edge detection, circle Hough transform, you can draw the cherry’s center and circular contour, to carry out the purpose of the machine picking. The system is simple and effective.

  3. An evaluation of Panicum maximum cv. Gatton: 2. The influence of ...

    African Journals Online (AJOL)

    Unknown

    Abstract. The aim of the study was to evaluate the nutritional value of Panicum maximum cv. .... Table 2 Mean (± s.d.) chemical composition of oesophageal samples .... solubility, energy content of the diet and level of intake (Van Soest, 1982), ...

  4. VizieR Online Data Catalog: 72 faint CV candidates in CRTS (Breedt+, 2014)

    Science.gov (United States)

    Breedt, E.; Gansicke, B. T.; Drake, A. J.; Rodriguez-Gil, P.; Parsons, S. G.; Marsh, T. R.; Szkody, P.; Schreiber, M. R.; Djorgovski, S. G.

    2016-04-01

    We obtained identification spectra of a total of 72 faint CV candidates identified by the CRTS, using the Gran Telescopio Canarias (GTC; La Palma, Spain) and the Gemini telescopes (North: Mauna Kea, Hawaii and South: Cerro Pachon, Chile). The observations were carried out in service mode during 2010, 2011 and 2013. (5 data files).

  5. STRATEGI SEGMENTASI PASAR TERHADAP VOLUME PENJUALAN BUSANA MUSLIM PADA CV RABBANI ASYSA MAKASSAR

    OpenAIRE

    KARASE, HASNAWATI

    2014-01-01

    2014 Hasnawati Karase Haris Maupa Mukhtar Penelitian ini bertujuan untuk menganalisis strategi segmentasi pasar terhadap volume penjualan busana muslim pada CV Rabbani Asysa Makassar. Data penelitian ini diperoleh dari kuesioner (primer) mengenai segmentasi pasar Rabbani Makassar dengan mengambil sampel sebanyak 75 responden. Temuan penelitian ini menunjukkan bahwa variabel strategi segmentasi pasar yang terdiri dari segmentasi geografis, segmentasi demografis, segm...

  6. Using CV-GLUE procedure in analysis of wetland model predictive uncertainty.

    Science.gov (United States)

    Huang, Chun-Wei; Lin, Yu-Pin; Chiang, Li-Chi; Wang, Yung-Chieh

    2014-07-01

    This study develops a procedure that is related to Generalized Likelihood Uncertainty Estimation (GLUE), called the CV-GLUE procedure, for assessing the predictive uncertainty that is associated with different model structures with varying degrees of complexity. The proposed procedure comprises model calibration, validation, and predictive uncertainty estimation in terms of a characteristic coefficient of variation (characteristic CV). The procedure first performed two-stage Monte-Carlo simulations to ensure predictive accuracy by obtaining behavior parameter sets, and then the estimation of CV-values of the model outcomes, which represent the predictive uncertainties for a model structure of interest with its associated behavior parameter sets. Three commonly used wetland models (the first-order K-C model, the plug flow with dispersion model, and the Wetland Water Quality Model; WWQM) were compared based on data that were collected from a free water surface constructed wetland with paddy cultivation in Taipei, Taiwan. The results show that the first-order K-C model, which is simpler than the other two models, has greater predictive uncertainty. This finding shows that predictive uncertainty does not necessarily increase with the complexity of the model structure because in this case, the more simplistic representation (first-order K-C model) of reality results in a higher uncertainty in the prediction made by the model. The CV-GLUE procedure is suggested to be a useful tool not only for designing constructed wetlands but also for other aspects of environmental management.

  7. STRATEGI SEGMENTASI PASAR TERHADAP VOLUME PENJUALAN BUSANA MUSLIM PADA CV RABBANI ASYSA MAKASSAR

    OpenAIRE

    KARASE, HASNAWATI

    2014-01-01

    2014 Hasnawati Karase Haris Maupa Mukhtar Penelitian ini bertujuan untuk menganalisis strategi segmentasi pasar terhadap volume penjualan busana muslim pada CV Rabbani Asysa Makassar. Data penelitian ini diperoleh dari kuesioner (primer) mengenai segmentasi pasar Rabbani Makassar dengan mengambil sampel sebanyak 75 responden. Temuan penelitian ini menunjukkan bahwa variabel strategi segmentasi pasar yang terdiri dari segmentasi geografis, segmentasi demografis, segm...

  8. Abstract Morphemes and Lexical Representation: The CV-Skeleton in Arabic

    Science.gov (United States)

    Boudelaa, Sami; Marslen-Wilson, Willian D.

    2004-01-01

    Overlaps in form and meaning between morphologically related words have led to ambiguities in interpreting priming effects in studies of lexical organization. In Semitic languages like Arabic, however, linguistic analysis proposes that one of the three component morphemes of a surface word is the CV-Skeleton, an abstract prosodic unit coding the…

  9. Agrobacterium-mediated transformation of Easter lily (Lilium longiflorum cv. Nellie White)

    Science.gov (United States)

    Conditions were optimized for transient transformation of Lilium longiflorum cv. Nellie White using Agrobacterium tumefaciens. Bulb scale and basal meristem explants were inoculated with A. tumefaciens strain AGL1 containing the binary vector pCAMBIA 2301 which has the uidA gene that codes for ß-gl...

  10. Isolation and characterization of CvIV4: a pain inducing α-scorpion toxin.

    Directory of Open Access Journals (Sweden)

    Ashlee H Rowe

    Full Text Available BACKGROUND: Among scorpion species, the Buthidae produce the most deadly and painful venoms. However, little is known regarding the venom components that cause pain and their mechanism of action. Using a paw-licking assay (Mus musculus, this study compared the pain-inducing capabilities of venoms from two species of New World scorpion (Centruroides vittatus, C. exilicauda belonging to the neurotoxin-producing family Buthidae with one species of non-neurotoxin producing scorpion (Vaejovis spinigerus in the family Vaejovidae. A pain-inducing α-toxin (CvIV4 was isolated from the venom of C. vittatus and tested on five Na(+ channel isoforms. PRINCIPAL FINDINGS: C. vittatus and C. exilicauda venoms produced significantly more paw licking in Mus than V. spinigerus venom. CvIV4 produced paw licking in Mus equivalent to the effects of whole venom. CvIV4 slowed the fast inactivation of Na(v1.7, a Na(+ channel expressed in peripheral pain-pathway neurons (nociceptors, but did not affect the Na(v1.8-based sodium currents of these neurons. CvIV4 also slowed the fast inactivation of Na(v1.2, Na(v1.3 and Na(v1.4. The effects of CvIV4 are similar to Old World α-toxins that target Na(v1.7 (AahII, BmK MI, LqhIII, OD1, however the primary structure of CvIV4 is not similar to these toxins. Mutant Na(v1.7 channels (D1586A and E1589Q, DIV S3-S4 linker reduced but did not abolish the effects of CvIV4. CONCLUSIONS: This study: 1 agrees with anecdotal evidence suggesting that buthid venom is significantly more painful than non-neurotoxic venom; 2 demonstrates that New World buthids inflict painful stings via toxins that modulate Na(+ channels expressed in nociceptors; 3 reveals that Old and New World buthids employ similar mechanisms to produce pain. Old and New World α-toxins that target Na(v1.7 have diverged in sequence, but the activity of these toxins is similar. Pain-inducing toxins may have evolved in a common ancestor. Alternatively, these toxins may be the

  11. Micromation and Structural Characteristics of Plantlets In vitro%试管植物器官微型化及结构特征

    Institute of Scientific and Technical Information of China (English)

    赵乐乐; 苏晴; 王玉海; 李友勇

    2011-01-01

    植物组织培养中的试管植物,其形态比自然形态要小得多,脱离试管后能恢复原形态大小,这种微型化和微型化解除是植物与环境相互作用的一个新命题.以试管马铃薯无性系微型薯为材料,在试管和花盆两种环境下发芽并长成植株,显微切片方法观察其组织结构特征,聚丙烯酰胺凝胶电泳(SDS-PAGE)方法分离不同处理植株的差异蛋白.研究发现,试管植株器官微型化的主要原因是细胞数量减少,其次是细胞体积减小,但试管植株的气孔则较大.在不同处理的植株中检测出单个和多个蛋白差异.结果说明,试管因子,包括营养和生长空间因子,能显著胁迫植物正常生长和形态建成,形态改变过程伴随有较大的生理变化.%It was frequently seen that plant morphogenesis in vitro was much smaller than that in the natural environment, and when they were moved out from the test tube, their organs could recover to natural size.This kind of micromation and unmicromation is a new proposition that could be used to research the interaction between plants and environment. The micropotatoes from the clones which was produced in vitro was act as the materials for observation of their morphological, structural, and physiologic characteristics as they were planted in both test-tube and flowerpot environment respectively. It was found that organs micromation under the stress in vitro was mainly because of the decrease of cell numbers, and also diminishing of cell size. But the stomata on test-tube plantlets were larger than on flowerpot plants. The results from SDS-PAGE were also found that a few differential proteins that were extracted from the plants stressed by nutrient and space factors were hanged on the gels. These results definitely indicated that the nutrition and space factors could significantly change plant growth and morphogenesis, and the process of morphogenesis was coupled with a variety of changes of

  12. Stimulation of somatic embryogenesis and plant regeneration from anther culture of Vitis vinifera cv. Cabernet-Sauvignon.

    Science.gov (United States)

    Cl Mauro, M; Nef, C; Fallot, J

    1986-10-01

    Somatic embryogenesis and subsequent diploid plants have been obtained from anthers of Vitis vinifera Cabernet-Sauvignon, a cultivar so far considered as recalcitrant to in vitro regeneration. Anthers enclosing microspores near the first pollen mitosis were found to be the most responsive. However, from a practical point of view anther length proved to be an easier criterium for determining the optimal physiological anther stage. Calli derived from the anther somatic tissues produced embryoids only when cultured on a medium supplemented with casein hydrolysate. Glutamine and adenine were found to stimulate this embryoid production. Evidence is presented that early removal of cotyledons increases the frequency of normal development of embryoids into plantlets.

  13. ANALISIS KEBUTUHAN TENAGA KERJA RESERVASI TERHADAP PERENCANAAN PENGEMBANGAN SUMBER DAYA MANUSIA PADA CV. CAN TOUR & TRAVEL

    Directory of Open Access Journals (Sweden)

    Maya Christina

    2017-01-01

    Full Text Available Employment remains the fundamental probel faced by many countries including Indonesia. One of the efforts the Government of Indonesia in extending employment opportunities is to develop the tourism sector. Bali is one of the potential tourist destinastions and it needs to be marketed. Cooperation between the Travel Bureau with the provider of the accommodation or hotel is one of the ways the potential market and the most instrumental in the development of the company, namely human resources required or called by labor. CV. CAN Tour and Travel is one of the tourist industry in Bali trip with a number of guest handling that quite a lot. This research aims to know the availability of labor needs resevartion in order CV. CAN Tour and Travel development and also to find out how the workforce qualification is required. Determination technique using the key informant that was labor reservation CV. CAN Tour and Travel, while the base of the informant was CV. CAN Tour and Travel Manager. Data analysis technique used is the analysis of workload and workforce needs analysis. The research results obtained indicate that shortage of time working as many as 210 minutes. So the manpower needed by CV. CAN Tour and Travel is two people with the fact that there is only 1 nowaday. It can be seen from the burden of the work done in a day’s work and the workforce absences. So labor needs on the Reservation Department is supposed to be 3 people, with 2 staff 1 reservations and ticketing staff. It was necessary increase manpower in the Labour Departmen’s Reservation in the reservation and also training to develop the ability to work.

  14. Beryllium-Boron Systematics of Refractory Inclusions in CR2 and CV3 Chondrites: Evidence for 10Be Heterogeneity

    Science.gov (United States)

    Dunham, E.; Wadhwa, M.; Simon, S.; Grossman, L.

    2016-08-01

    Be-B systematics of Allende (CV3), Axtell (CV3), and NWA 5028 (CR2) CAIs suggests that 10Be was distributed heterogeneously in the early solar system which implies that 10Be was produced in the solar nebula by irradiation of nebular gas or dust.

  15. Micropropagação de plântulas de erva-mate obtidas de embriões zigóticos Micropropagation of holly plantlets obtained from zygotic embryos

    Directory of Open Access Journals (Sweden)

    Micheli Angélica Horbach

    2011-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o potencial de micropropagação de erva-mate (Ilex paraguariensis Saint Hilaire a partir de plântulas assépticas oriundas de embriões zigóticos imaturos. Ápices caulinares foram inoculados em meio base, formado por ¼ da concentração dos sais do meio MS, com diferentes doses de 6-benzilaminopurina (BAP. Ápices caulinares foram enraizados em meio base com diferentes doses de ácido indolbutírico (AIB e avaliado o efeito do AIB do meio de enraizamento in vitro na aclimatização das plantas. O meio base com 8,88µM de BAP foi o mais efetivo para a multiplicação, promovendo o maior número e comprimento de brotações adventícias. Ápices caulinares enraizaram em 30 dias de cultivo em meio base, acrescido de 7,38µM de AIB. A dose de AIB utilizada no enraizamento in vitro interfere na aclimatização das plantas. A técnica de micropropagação descrita pode ser utilizada para a produção massal de plantas de erva-mate.The aim of this research was to evaluate the micropropagation potential of holly (Ilex paraguariensis Saint Hilaire aseptic plantlets derived from immature zygotic embryos. Shoot tip were inoculated in a quarter-strength MS basal medium with different doses of 6-benzylaminopurine (BAP to induce multiple shoots. Shoot tips were inoculated in a basal medium with different doses of indol-butiric acid (IBA for in vitro rooting and to test their effects in plantlet acclimatization. The addition of 8.88µM BAP to the basal medium increased the number of adventitious shoots and shoot height. Shoot tip rooted in basal medium with 7.38µM IBA in 30 days of culture. The IBA doses of the rooting medium affect plantlet acclimatization. The described micropropagation protocol might be used to produce a high number of holly plants.

  16. Growth performance on regenerated plantlets of Pinus densiflora resistance to pine wilt disease%抗松材线虫病赤松组培再生植株田间生长表现

    Institute of Scientific and Technical Information of China (English)

    秦莉; 吴小芹; 朱丽华; 叶建仁; 张红岩

    2011-01-01

    In order to evaluate the performance of regenerated plantlets of Pinus densiflora resistance to pine wilt disease,research has advanced to the field testing stage. The results showed that 0.5 year-old plantlets survival rate was apparently lower than that of 2.5,2,1.5,1 year-old. There were obvious growth difference among different clones. Mycorrhizal foresting test indicated that ectomycorrhizal fungi Rhizopogen luteous and Boletus edulis facilitated the growth of disease-resistant P. densiflora 1 # -A 5 and 8 generation. This research provided reference for breeding and afforestation of regenerated plantlets of P. densiflora resistance to pine wilt disease.%为了评价从日本引进的抗松材线虫病赤松通过无性快繁获得的再生植株在我国的生长状况.本研究对田间试种的抗病赤松6个家系12个无性系共688株再生植株进行跟踪监测.结果表明,抗病赤松0.5年生再生植株试种成活率明显低于2.5年、2年、1.5年及1年生再生植株;不同无性系间抗病赤松再生植株生长状况存在明显差异.其中,1#-A和5#-5无性系再生植株生长较好.菌根化造林试验表明,两种外生菌根菌美味牛肝菌(Be)和黄色须腹菌(R1)对抗病赤松1#-A5代、8代再生植株具有明显促生效果.此研究为今后抗病赤松再生植株造林育种提供了参考依据.

  17. Effects of Different Treatments on the Transplanting of Wine Grape 'Ecolly' Plantlets%不同处理对酿酒葡萄品种'爱格丽'组培苗移栽的影响

    Institute of Scientific and Technical Information of China (English)

    李华; 苏娟; 王华; 王艳妮

    2011-01-01

    试验研究了不同处理对'爱格丽'组培苗移栽成活率、生长形态及生理指标的影响.利用常规的方法,测定株高等生长指标,TTC等生理试验方法测定根系活力等生理指标.结果表明:腐殖土:珍珠岩1:1(体积比)的移栽成活率和抗逆性最高,腐殖土:珍珠岩:园土:牛粪1:1:1:0.3(体积比)的干重比和根系活力最好.移栽器皿的大小对植株的生长有着一定的影响,0.4 g·L-1的激素短时间对促发新根作用不太明显,对于移栽成活的组培苗,0.4 g·L-1的激素可以提高其根系活力和壮苗指数.就大范围的推广,腐殖土:珍珠岩:园土:牛粪1:1:1:0.3适宜作为'爱格丽'组培苗移栽的基质.%Effects of different substrates on the survival rate, growth and physiological characteristics of ‘ Ecolly’ were studied. Growth indices were determined by routine methods. Physiological indices were determined by TTC and other methods. The highest survival rate and stress-resistance of the transplanted plantlets were obtained in the mixed substrate of humus soil and perlite with a proportion of 1: 1 (v/v), and the dried weight ratio and root vigor were the highest in the mixed substrate of humus soil, perlite, soil, and dung with a proportion of 1 : 1 : 1 : 0. 3 (v/v). The size of transplanting container exhibited some influences on the growth of the plantlets. The effect of hormone with a concentration of 0. 4 g · L-1 on promoting new roots was not obvious in short time, but it can improve root vigor and healthy seedling index of the survived plantlets. For the large-scale transplanting, the mixed substrate of humus soil, perlite, soil, and dung with a proportion of 1: 1 : 1: 0.3 was more suitable than humus soil and perlite (1: 1).

  18. Effects of Coconut Milk on Plantlet Hyperhydricity of Cymbidium Hybrid%椰汁对大花蕙兰超含水态苗发生影响研究

    Institute of Scientific and Technical Information of China (English)

    杨传贵; 刘景然; 刘雪梅; 高玉葆

    2011-01-01

    Chose 4 hybrid groups of Cymbidium, and explored the effects of coconut milk on hyperhydricity of Cymbidium plantlet in vitro. Reveals firstly that coconut milk is an important factor causing hyperhydricity of Cymbidium plantlet. It showed that different hybrid groups differed in hyperhydricity occurrence with the content variation of coconut milk. The highest rate reached 37. 0%, while the lowest one was zero. Coconut milk has great effects on the occurrence of hyperhydricity. When the quantity of 6-BA and IBA were fixed, the number of plantlets infected by hyperhydricity increased with the increasing of coconut milk within 3. 0%~6. 0%, in which the highest hyperhydricity index got 26. 0. We realize that the hormone , sugars, and amino acids contained in coconut milk raised the level of hormone,sugars, and NH concentrations in the culture media, and resulted high hyperhydricity occurrence. The paper created the idea of hyperhydricity index which evaluates the occurrence of hyperhydricity, and get accurate and better appraisement.%研究了椰汁对4个大花蕙兰杂交系无菌播种超含水态苗发生的影响,发现椰汁是引起大花蕙兰小植株超含水态发生的重要因素.椰汁用量不同,不同杂交组合超含水态发生情况差异较大,最高发生率达到37.0%,最低为零.椰汁对超含水态的发生具有重要影响.当激素6-BA和IBA用量一定时,在3.0%~6.0%范围内,随着椰汁用量的增加,超含水态苗数量升高,超含水态发生指数最高达到26.0.分析表明,椰汁中含有的激素、糖类和氨基酸,引起培养基中激素、糖类和铵离子浓度的增加,进而引起超含水态现象.

  19. Tratamentos físicos e químicos na emergência e no crescimento de plântulas de pinheira Physical and chemical treatments on emergency and growth of sweetsop plantlets

    Directory of Open Access Journals (Sweden)

    Silvia Nietsche

    2005-01-01

    Full Text Available O objetivo do presente trabalho foi verificar o efeito do armazenamento e a influência de tratamentos físicos e químicos sobre a emergência e o crescimento de plântulas de pinheira. Frutos de pinheiras foram coletados em um pomar comercial no município de Nova Porteirinha, Minas Gerais. Os frutos colhidos maduros foram despolpados e suas sementes, lavadas em água corrente. O delineamento utilizado foi o de blocos casualizados, em um esquema fatorial, com cinco tratamentos (testemunha, imersão em vinagre, escarificação com lixa, escarificação em liquidificador e desponte com alicate e quatro épocas de armazenamento em geladeira (0, 2, 4 e 6 meses. Trinta dias após o plantio de cada época, foi avaliada a porcentagem de plântulas emergidas, tempo médio para emergência, número de folhas, diâmetro, altura das plantas, matéria fresca e matéria seca da parte aérea e do sistema radicular. Dentre os principais resultados pode-se destacar que não foram observados efeitos significativos dos tratamentos na quebra de dormência das sementes de pinheira e o armazenamento em geladeira por seis meses não prejudicou a emergência e o desenvolvimento das plântulas de pinheira.This study aimed verifying the effect of the storage and the influence of physical and chemical treatments on emergency and growth of sweetsop plantlets. Sweetsop fruits were collected in a commercial orchard in Nova Porteirinha District, Minas Gerais. Fruits were picked when ripe, the pulp was extracted and the seeds washed. The design was in blocks at random, in a factorial system with five treatments (control, immersion in vinegar, sandpaper scarification, scarification in blender and cutting with pliers and four periods of storage at 4 ºC (0, 2, 4 e 6 months. Percentage of surfaced plantlets, average time to emergency, number of leaves, diameter, plant height, shoot root fresh and dry matter were evaluated thirty days after the planting of each period. Among

  20. Estudo da influência da época de coleta dos ramos, no enraizamento de etacas caulinares de café (Coffea arabica L. cv "Mundo Novo" Influence of branche harvest period in rooting of coffee stem cuttings (Coffea arabica L. cv "Mundo Novo"

    Directory of Open Access Journals (Sweden)

    E.O. Ono

    1992-01-01

    Full Text Available Na propagação vegetativa através de estacas caulinares, a época de retirada dos ramos, que serão utilizados na confecção das estacas, tem grande influência sobre seu enraizamento. Outro fator de grande relevância é o tratamento auxínico, realizado nas estacas, que promove a formação de raízes. O presente trabalho teve como objetivo, verificar a ação das auxinas e do boro e a influência da época de coleta dos ramos de cafeeiro, sobre o enraizamento de ramos ortotrópicos semi-lenhosos de café (Coffea arabica L. cv "Mundo Novo". Para tal, o experimento foi montado mensalmente, realizando-se as seguintes medidas , mediante coleta das estacas, após 90 dias do plantio: número de estacas enraizadas e número total de raízes formadas. À partir dos resultados obtidos, concluiu-se que, a melhor época de coleta dos ramos é na estação chuvosa. Além disso, tratamentos com NAA à 200 ppm mais boro, também promovem uma alta porcentagem de enraizamento.Timing of stem cuttings has large influence on rooting and on vegetative propagation of coffee seedlings. Auxin treatments on cuttings also promote root formation. The present research evaluated auxins and boron actions and the influence time of coffee-tree branch havest time, over rooting of orthotropous semi-hardwood branches of coffee (Coffea arabica L. cv . Mundo Novo stem cuttings. The trial was performed monthly, observing the cuttings 90 days after planting: number of rooted cuttings and total root number. It was concluded that the best branch harvesting time is in the rainy season, whem cuttings show satisfatory results. Treatments with NAA 200 ppm plus boric acid, promoted higher rooting percentage.

  1. Storage of cut Heliconia bihai (L. cv. Lobster Claw flowers at low temperatures Armazenamento de hastes florais de Heliconia bihai (L. cv. Lobster Claw em baixa temperatura

    Directory of Open Access Journals (Sweden)

    Andreza S. Costa

    2011-09-01

    Full Text Available The postharvest conservation of cut Heliconia flowers is an important factor to the success of commercialization, especially with regard to exportation. In the present study, the maximal storage time of cut inflorescences of Heliconia bihai cv. Lobster Claw at two different temperatures (12 and 19 °C was evaluated and compared to laboratory conditions (25 °C, control treatment. Changes in visual quality, fresh weight and bract color (L*, a* and b* were determined. The visual quality of the inflorescences and fresh weight decreased with time in all treatments. Symptoms of chilling injury were observed on the inflorescences stored at 12 °C for six and eight days. Bract color was not affected by temperature, storage time or the senescence process. The results indicate that a temperature of 12 °C is not recommended for a storage time longer than four days, whereas 19 °C can be used for a storage time of up to eight days for cut inflorescences of H. bihai cv. Lobster Claw.A conservação pós-colheita de flores de corte de Heliconia é fator relevante para o sucesso da comercialização, principalmente para a exportação. Neste estudo, o período máximo de armazenamento de hastes florais de Heliconia bihai cv. Lobster Claw, foi avaliado em duas diferentes temperaturas (12 e 19 °C e comparado com as condições de laboratório (25 °C, tratamento controle. As variáveis avaliadas foram: qualidade visual, massa de matéria fresca e a coloração das inflorescências (L*, a* e b*. A qualidade visual das inflorescências e a massa de matéria fresca de todos os tratamentos reduziram ao longo do tempo. Sintomas de injúria por frio foram observados nas inflorescências armazenadas a 12 °C, durante seis e oito dias. A coloração das brácteas não foi afetada pela temperatura, período de armazenamento nem pelo processo de senescência. Os resultados indicam que a temperatura de 12 °C não é recomendada para armazenar hastes florais de

  2. Comportamento agronômico inicial da cv. Chimarrita enxertada em cinco porta-enxertos de pessegueiro Agronomical behavior of cv. Chimarrita on five rootstocks of peach trees

    Directory of Open Access Journals (Sweden)

    Moacir da Silva Rocha

    2007-01-01

    Full Text Available O trabalho teve por objetivo avaliar a resposta agronômica da cv. Chimarrita enxertada em cinco porta-enxertos, nas condições edafoclimáticas da região de Pelotas-RS, no período de 2003 a 2005. Durante o período de execução do experimento, foram avaliados o diâmetro do tronco do porta-enxerto e da cultivar-copa, comprimento médio dos ramos principais, volume de copa, massa fresca e massa seca do material vegetal retirado nas podas verde e de inverno, índice de intensidade de poda, massa média dos frutos, produção por planta, eficiência produtiva, produção por hectare, sólidos solúveis totais, firmeza da polpa, diâmetro e coloração dos frutos. O porta-enxerto 'Capdeboscq' induziu o maior crescimento vegetativo na cv. Chimarrita durante os três anos de avaliação, seguido do porta-enxerto 'Okinawa'. Este, por sua vez, induziu o maior rendimento produtivo (1,65 t ha-1. A cv. Capdeboscq proporcionou a obtenção de frutos com maior massa. Os porta-enxertos 'GF 305' e 'Aldrighi' induziram menor desenvolvimento vegetativo e a mais baixa produtividade (0,52 t.ha-1 .The objective for this study was to evaluate the agronomical behavior of cv. Chimarrita on five rootstocks of peach trees in Pelotas- RS from 2003 to 2005. The variables evaluated were: trunk diameters of the rootstock and of the scion; length of the annual terminal growth; volume of the canopy; fresh and dry weight of the material taken by the summer and winter pruning; pruning intensity index; fruit weight; fruit production per tree; production efficiency; productivity per hectare; total soluble solids; pulp firmness; and fruit diameter and color. It was observed that the trees from: rootstock 'Capdeboscq' promoted higher vegetative growth and larger fruits, followed by the ones from the rootstock 'Okinawa' which promoted the highest yield (1, 65 t.ha-1; the rootstock 'GF 305' and 'Aldrighi' had the lowest vegetative development and yield (0, 52 t.ha-1.

  3. [The estimation of daily physical activity with the coefficient of variation (CV) of heart rates continuously recorded].

    Science.gov (United States)

    Tono-oka, T; Kaneko, I

    1993-05-01

    The daily level of physical activity was estimated using the heart rate monitor, PE3000 (Polar Electro, Finland). The level was expressed with the coefficient of variation (CV) of heart rates recorded from waking time to dinner time. In the course of a day of intense physical activity, CV was confirmed to rise significantly. Then the CV was estimated and compared among 3 age classes, young (10-18 years), middle-aged (30-47 years), and elderly (62-76 years). The CVs of young people were significantly higher than those of middle-aged (P < 0.001) and elderly (P < 0.01), regardless of sex. However there was no significant sex difference in all age classes. These results suggest that the CV is an accurate index of daily physical activity. Thus clinicians can use the CV of heart rates to estimate the level of physical activity of individuals which closely relates to QOL.

  4. Estudo de simulações de microclimas em casas de vegetação visando à aclimatação de mudas micropropagadas de bananeira cv Grande Naine Simulations of microclimate in greenhouses aiming the acclimatization of micropropagated seedlings of banana cv. Grande Naine

    Directory of Open Access Journals (Sweden)

    Ciro Scaranari

    2008-12-01

    Full Text Available Em processos de aclimatação, o controle ambiental assume papel de vital importância, pois, ainda na condição in vitro, as plantas não operam eficientemente a absorção de luz, água e nutrientes. Devem, portanto, ser submetidas a ambientes controlados sob condições favoráveis de luminosidade, temperatura (ao redor de 28ºC, com mínimas a 18ºC e máximas a 34ºC e umidade relativa (acima de 75%. Para tanto, foram construídos 5 mini-túneis com temperatura e umidade relativa controladas. No controle da temperatura, usaram-se resfriadores evaporativos do tipo ventilador-meio poroso, 28/25ºC. No controle da umidade relativa, usou-se nebulização durante o dia a 75% sob intermitência de 6s a cada 40s. Para o monitoramento da temperatura e umidade relativa, foram instalados 3 psicrômetros aspirados em cada casa de vegetação, ligados a sistema programado para leituras diárias com partição de 60s. Os resultados indicam controle satisfatório nos ambientes, oferecendo condições favoráveis para as plantas de bananeira sob o 2º estágio de aclimatação, embora tenham sido observadas diferenças significativas entre eles. Para o estudo da luminosidade, sob filme plástico transparente PEBD de 100µm, foram utilizadas telas com média de sombreamento na faixa RFA (400 a 700nm de 69,92%, 50,73%, 29,73% e 57,77%, sendo as 3 primeiras de cor vermelha (com picos na faixa de 580nm e redução abrupta a partir daí, e a última de cor preta (comportamento linear, respectivamente. O 5º ambiente contou apenas com o filme, apresentando 12,74% de interceptação da radiação solar. Esses valores foram obtidos a partir de amostras pareadas, tela e filme para os 4 primeiros ambientes e apenas o filme para o último, utilizando-se de espectro-radiômetro programado para a faixa de 400 a 1.100nm, com resolução espectral de 2nm. Dentro e fora de cada ambiente, obtiveram-se dados de irradiâncias RFA e global, por meio de sensores fotovoltaicos de silício, por volta das 9h, 12h e 15h, sob condições de céu claro e encoberto, durante o verão de 2004/05 e inverno de 2005. As malhas vermelhas apresentam transmitâncias diferenciadas ao longo do espectro da radiação fotossinteticamente ativa, constituindo-se assim em interessantes materiais para os pretendidos estudos sobre aclimatação. Resultados mostram maiores reduções na faixa RFA para as telas vermelhas, independentes do horário, insolação e estação do ano, concordantes com aqueles obtidos por espectro-radiômetro.In acclimatization processes, the environment control takes over a part of vital importance, since in in vitro condition banana seedlings do not efficiently operate the absorption of light, water and nutrients. Therefore, they should be submitted to controlled environments under feasible conditions of luminosity, temperature (around 28ºC, with minimum of 18ºC and maximum of 34ºC, and relative humidity above 75%. For this purpose, it was installed five mini tunnels with controlled temperature and relative humidity. For the control of temperature it was used pad-fan evaporative coolers at 28/25ºC. For the control of relative humidity it was used fogging during the day at 75% under intermittence of 6s at each 40s. For temperature and relative humidity monitoring, it was installed three aspirate psychrometers in each mini tunnel connected to a data logger for daily acquisition every 60s. Temperature and relative air humidity were adequate for plants development, however significant differences were observed between the different environment conditions. For luminosity study, under transparent plastic film (low density polyethylene of 100µm thickness, it was used four meshes with average shading in the PAR spectrum band (400 to 700nm of 69.92%, 50.73%, 29.73%, and 57.77%, being the first three in red color (with top level at 580nm, and abrupt reduction after that, the fourth mesh was black (linear behavior. The fifth tunnel had only transparent plastic film, showing 12.74% of solar radiation interception. These values were obtained from paired samples (mesh and plastic film for the first four tunnels, and only the transparent plastic film for the last, using a spectral radiometer (400 to 1100nm, with spectral resolution of 2nm. Inside and outside of each environment it was obtained global and PAR irradiance by using calibrated photovoltaic sensors at 9:00, 12:00, and 3:00, under clean and cloudy sky, during the summer of 2004/05, and winter of 2005. The red mesh presented differentiated transmittances at the PAR spectrum band which established an interesting material for acclimatization studies. Results show bigger reductions on the PAR spectrum band for the red mesh, regardless of time, solar radiation, or seasons, which agree with the results obtained by the spectral radiometer

  5. [Massive multiplication of coffee (Coffee arabica L. cv. Catimor) through embryogenic cell suspension culture].

    Science.gov (United States)

    Flermoso-Gallardo, L; Menóndez-Yuffá, A

    2000-01-01

    Cell suspensions offer several advantages as a system for massive propagation because of the high rates of multiplication, the higher homogeneity in the culture conditions and the possibility of automatization. In this study, different experimental conditions were analyzed to establish embryogenic cell suspension cultures of coffee. The best conditions to establish the embryogenic cell suspension cultures of coffee were as follows: coffee leaf sections were cultivated during 12 weeks (Stage I) in a solid medium with the Murashige and Skoog salts, 2 mg/l kinetin and 0.5 mg/l 2,4-dichlorophenoxiacetic acid (medium 1). Under these conditions the explants formed a callus tissue that was transferred to a liquid medium containing 5 mg/l of 6-benzylamlno-purine (medium 2). After 12 days in a shaking liquid medium (Stage II), the cultures were sieved and were maintained In the same media, which was renewed every eight days (Stage III). This method yielded 1884 embryos in 50 ml; placing the embryos under conditions for germination yielded plantlets of normal appearance.

  6. Grand Canal:Jiaxing in Action for World Heritage Listing

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    Editor’s Note: cities along the Grand Canal of China are working together to apply for a world heritage status for the world’s longest man-made waterway. Jiaxing in northern Zhejiang is in the league of cities for this grand mission. Two spots and six water-ways of Jiaxing are to go into the joint application. The author of this feature story is a journalist with Jiaxing Daily. From 2009 to 2011, he traveled all the way from Hangzhou to Beijing. For over two and half a year, he covered all the sections of the canal and cultural heritages, experienced local customs and lifestyles, interviewed 100 masters designated as inheritors of intangible cultural heritages. The following is a brief account of the Jiaxing section of the Grand Canal.

  7. Biomechanical analysis technique choreographic movements (for example, "grand battman jete"

    Directory of Open Access Journals (Sweden)

    Batieieva N.P.

    2015-04-01

    Full Text Available Purpose : biomechanical analysis of the execution of choreographic movement "grand battman jete". Material : the study involved students (n = 7 of the department of classical choreography faculty of choreography. Results : biomechanical analysis of choreographic movement "grand battman jete" (classic exercise, obtained kinematic characteristics (path, velocity, acceleration, force of the center of mass (CM bio parts of the body artist (foot, shin, thigh. Built bio kinematic model (phase. The energy characteristics - mechanical work and kinetic energy units legs when performing choreographic movement "grand battman jete". Conclusions : It was found that the ability of an athlete and coach-choreographer analyze the biomechanics of movement has a positive effect on the improvement of choreographic training of qualified athletes in gymnastics (sport, art, figure skating and dance sports.

  8. Liberating methodological thinking in human sciences from grand theories

    DEFF Research Database (Denmark)

    Kharlamov, Nikita; Baldursson, Einar Baldvin

    2016-01-01

    Many humanistic and social disciplines are naturally inclined to seek for human-, person-, self- centered focus, and develop a holistic theory of such. Such disciplines continually engage with philosophical, metaphysical and meta-theoretical perspectives. This engagement often leads to a singular...... focus on the necessity of a “grand unified theory” at the expense of any and all alternative perspectives. Properties of grand theories are discussed on the examples of Giddens and Bourdieu. It is argued that grand theories hamper a more productive focus on concrete phenomena. Robert Merton’s focus...... on “middle range” theories is revisited and its continuing relevance is highlighted. The level of abstraction characteristic of such theories, as well as the way they engage with the empirical social reality, are discussed. The article concludes by considering the paradoxical reductionism that can...

  9. Acúmulo, exportação e restituição de nutrientes pelas bananeiras "Prata Anã" e "Grand Naine" Accumulation, export and restitution of nutrients by 'Prata Anã' and 'Grand Naine' bananas

    Directory of Open Access Journals (Sweden)

    Frederico Antonio Loureiro Soares

    2008-10-01

    in case of 'Prata Anã' was K>N>Ca>P>Mg while in Grand Naine cultivar the sequence was of K>Ca>N>P>Mg. At harvest time, in the clumps of cv. Prata Anã were accumulated on an average 2.2 times more nutrients in comparison to mother plant while in the plants of Grand Naine such relation was 1.2 times. Independent of cultivar, the order of the amount of nutrients returned to the soil after the crop removal was K>Ca>N>Mg>P.

  10. Regional economic impacts of Grand Canyon river runners.

    Science.gov (United States)

    Hjerpe, Evan E; Kim, Yeon-Su

    2007-10-01

    Economic impact analysis (EIA) of outdoor recreation can provide critical social information concerning the utilization of natural resources. Outdoor recreation and other non-consumptive uses of resources are viewed as environmentally friendly alternatives to extractive-type industries. While outdoor recreation can be an appropriate use of resources, it generates both beneficial and adverse socioeconomic impacts on rural communities. The authors used EIA to assess the regional economic impacts of rafting in Grand Canyon National Park. The Grand Canyon region of northern Arizona represents a rural US economy that is highly dependent upon tourism and recreational expenditures. The purpose of this research is twofold. The first is to ascertain the previously unknown regional economic impacts of Grand Canyon river runners. The second purpose is to examine attributes of these economic impacts in terms of regional multipliers, leakage, and types of employment created. Most of the literature on economic impacts of outdoor recreation has focused strictly on the positive economic impacts, failing to illuminate the coinciding adverse and constraining economic impacts. Examining the attributes of economic impacts can highlight deficiencies and constraints that limit the economic benefits of recreation and tourism. Regional expenditure information was obtained by surveying non-commercial boaters and commercial outfitters. The authors used IMPLAN input-output modeling to assess direct, indirect, and induced effects of Grand Canyon river runners. Multipliers were calculated for output, employment, and income. Over 22,000 people rafted on the Colorado River through Grand Canyon National Park in 2001, resulting in an estimated $21,100,000 of regional expenditures to the greater Grand Canyon economy. However, over 50% of all rafting-related expenditures were not captured by the regional economy and many of the jobs created by the rafting industry are lower-wage and seasonal. Policy

  11. Connection between solar activity cycles and grand minima generation

    Science.gov (United States)

    Vecchio, A.; Lepreti, F.; Laurenza, M.; Alberti, T.; Carbone, V.

    2017-03-01

    Aims: The revised dataset of sunspot and group numbers (released by WDC-SILSO) and the sunspot number reconstruction based on dendrochronologically dated radiocarbon concentrations have been analyzed to provide a deeper characterization of the solar activity main periodicities and to investigate the role of the Gleissberg and Suess cycles in the grand minima occurrence. Methods: Empirical mode decomposition (EMD) has been used to isolate the time behavior of the different solar activity periodicities. A general consistency among the results from all the analyzed datasets verifies the reliability of the EMD approach. Results: The analysis on the revised sunspot data indicates that the highest energy content is associated with the Schwabe cycle. In correspondence with the grand minima (Maunder and Dalton), the frequency of this cycle changes to longer timescales of 14 yr. The Gleissberg and Suess cycles, with timescales of 60-120 yr and 200-300 yr, respectively, represent the most energetic contribution to sunspot number reconstruction records and are both found to be characterized by multiple scales of oscillation. The grand minima generation and the origin of the two expected distinct types of grand minima, Maunder and longer Spörer-like, are naturally explained through the EMD approach. We found that the grand minima sequence is produced by the coupling between Gleissberg and Suess cycles, the latter being responsible for the most intense and longest Spörer-like minima (with typical duration longer than 80 yr). Finally, we identified a non-solar component, characterized by a very long scale oscillation of 7000 yr, and the Hallstatt cycle ( 2000 yr), likely due to the solar activity. Conclusions: These results provide new observational constraints on the properties of the solar cycle periodicities, the grand minima generation, and thus the long-term behavior of the solar dynamo.

  12. 硝酸镧对霍霍巴多芽苗生长的促进作用及植株再生%STIMULATION OF SHOOT PROLIFERATION BY LANTHANUM NITRATE AND PLANT REGENERATION OF JOJOBA PLANTLET IN VITRO

    Institute of Scientific and Technical Information of China (English)

    徐进; 王玉珍; 罗景兰

    2004-01-01

    The plantlets in vitro of Jojoba were treated by 1~3.5 mg/L of Lanthanum nitrate on subculture medi um( MS containing 2 mg/L 6-BA and 0.5 mg/L GA3), the result showed a significant promotion on shoot growth and proliferation. 2.5 mg/L had a very significant effect on shoot differentiation. Roots formation could be improved by using 1~2 mg/L of Lanthanum nitrate on the rooting medium(MS1/2 supplemented with 3 mg/L NAA and 1.5 mg/L IBA), and 2 mg/L had a very significant effect. High concentration of Lanthanum nitrate inhibited the plantlets growth. The result indicated that different plant organs had different sensitivity on the concentration of Lanthanum nitrate. 2 cm high test-tube shoots were treated by 25 mg/L IBA or NAA for 30 min, then cultivated into sand substrate. Plantlets were elaborately reared under 20~ 30 ℃ in a greenhouse. Uncovered the plastic membrane after 50 d. Keep the light intensity at 3000 lx and relative hurnidity 85 % . The survival percent of the plantlets come up to 75 %.%在增殖培养基(改良MS+2 mg/L6-BA+0.5 mg/TGA3)中添加1~3.5 mg/L硝酸镧,对霍霍巴试管苗生长有显著促进作用,2.5 mg/L硝酸镧对多芽分化有极显著的促进;在生根培养基(改良1/2MS+3 mg/L IBA+1.5 mg/L NAA)中,1~2 mg/L的La(NO3)3对根的分化有显著的促进作用,生根率提高,最佳浓度为2.0 mg/L.过高的浓度对试管苗生长有一定抑制.试验表明,不同器官对硝酸镧的敏感程度不同.取2cm高以上的霍霍巴试管苗,用25 mg/L IBA或NAA处理30 min,扦插于沙基质中.保持室温20~30℃.50d后揭去覆膜,保持光强3000 lx,相对湿度85%以上.正常管理条件下成活率达75%.

  13. The formation of callus from papaya leaf and its generation induced and transplanting the plantlets with roots%番木瓜叶片愈伤组织形成、分化及再生植株移栽

    Institute of Scientific and Technical Information of China (English)

    朱西儒; 张云开

    2001-01-01

    The formation of callus from papaya leaf and the generation induced the tube seedling plantlets were studied in thispaper. It was one useful way and method of the gene trans-formation to papaya ring-spot virus (PRV) and to screening lines with storing for long time and good quality.%研究了番木瓜的叶片愈伤组织的形成,并进一步诱导分化,离体培养成完整的试管植株,这对深入进行体细胞突变育种,以及抗病毒品系筛选和种质改良或耐贮藏等基因转化,提供了有用的技术和方法。

  14. Grand Symmetry, Spectral Action, and the Higgs mass

    CERN Document Server

    Devastato, Agostino; Martinetti, Pierre

    2013-01-01

    In the context of the spectral action and noncommutative geometry approach to the standard model, we build a model based on a larger symmetry. This symmetry satisfies all the conditions to have a noncommutative manifold, and mixes gauge and spin degrees of freedom and does not introduce extra fermions. With this "grand symmetry" it is natural to have the scalar field necessary to obtain the Higgs mass in the vicinity of 126 GeV. The spectral action breaks the grand symmetry to the standard model algebra. This breaking also gives the spin structure of spacetime as broken symmetry.

  15. The Air-Shower Experiment KASCADE-Grande

    Energy Technology Data Exchange (ETDEWEB)

    Haungs, A., E-mail: haungs@ik.fzk.d [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Apel, W.D. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Arteaga, J.C. [Institut fuer Experimentelle Kernphysik, Universitaet Karlsruhe, D-76021 Karlsruhe (Germany); Badea, F.; Bekk, K. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Bertaina, M. [Dipartimento di Fisica Generale dell' Universita, 10125 Torino (Italy); Bluemer, J. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Institut fuer Experimentelle Kernphysik, Universitaet Karlsruhe, D-76021 Karlsruhe (Germany); Bozdog, H. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Brancus, I.M. [National Institute of Physics and Nuclear Engineering, P.O. Box Mg-6, RO-7690 Bucharest (Romania); Brueggemann, M.; Buchholz, P. [Fachbereich Physik, Universitaet Siegen, 57068 Siegen (Germany); Cantoni, E. [Dipartimento di Fisica Generale dell' Universita, 10125 Torino (Italy); Istituto di Fisica dello Spazio Interplanetario, INAF, 10133 Torino (Italy); Chiavassa, A. [Dipartimento di Fisica Generale dell' Universita, 10125 Torino (Italy); Cossavella, F. [Institut fuer Experimentelle Kernphysik, Universitaet Karlsruhe, D-76021 Karlsruhe (Germany); Daumiller, K. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany); Souza, V. de [Institut fuer Experimentelle Kernphysik, Universitaet Karlsruhe, D-76021 Karlsruhe (Germany); Di Pierro, F. [Dipartimento di Fisica Generale dell' Universita, 10125 Torino (Italy); Doll, P.; Engel, R.; Engler, J. [Institut fuer Kernphysik, Forschungszentrum Karlsruhe, D-76021 Karlsruhe (Germany)

    2009-12-15

    KASCADE-Grande is an extensive air shower experiment at the Forschungszentrum Karlsruhe, Germany. Main parts of the experiment are the Grande array spread over an area of 700x700m{sup 2}, the original KASCADE array covering 200x200m{sup 2} with unshielded and shielded detectors, and additional muon tracking devices. This multi-detector system allows to investigate the energy spectrum, composition, and anisotropies of cosmic rays in the energy range up to 1 EeV. An overview on the performance of the apparatus and first results will be given.

  16. The Grand Geochemistry of 4 Vesta: First Results

    Science.gov (United States)

    Prettyman, T. H.; Beck, A.; Feldman, W. C.; Forni, O.; Joy, S. P.; Lawrence, D. J.; McCoy T. J.; McFadden, L. A.; McSween, H. Y.; Mittlefehldt, D. W.; Polanskey, C. A.; Rayman, M. D.; Raymond, C. A.; Reedy, R. C.; Russell, C. T.; Titus, T. N.; Toplis, M. J.; Yamashita, N.

    2012-01-01

    On 12-Dec-2011, the Dawn spacecraft commenced low altitude mapping of the giant asteroid, 4 Vesta (264-km mean radius). Dawn's roughly circular, polar, low altitude mapping orbit (LAMO) has a mean radius of 470 km, placing the spacecraft within about 210 km of Vesta's surface. At these altitudes, Dawn s Gamma Ray and Neutron Detector (GRaND) is sensitive to Vesta's elemental com-position (Fig. 1). GRaND will acquire data in LAMO for up to 16 weeks, which is sufficient to map the elemental composition of the entire surface of Vesta. The timing of LAMO enables us to report the first results of our geochemistry investigation at this conference. In this abstract, we present an overview of our initial observations, based on data acquired at high altitude and during the first weeks of LAMO. GRaND overview. A detailed description of the GRaND instrument, science objectives and prospective results is given in [1]. At low altitudes, GRaND is sensitive to gamma rays and neutrons produced by cosmogenic nuclear reactions and radioactive decay occurring within the top few decimeters of the surface and on a spatial scale of a few hundred kilometers. From these nuclear emissions, the abundance of several major- and minor-elements, such as Fe, Mg, Si, K, and Th can be determined. Assuming the howardite, eucrite, and diogenite (HED) meteorites are representative of Vesta s crustal composition [2], then GRaND will be able to map the mixing ratios of whole-rock HED end-members, enabling the determination of the relative proportions of basaltic eucrite, cumulate eucrite, and diogenite as well as the proportions of mafic and plagioclase minerals [1,3]. GRaND will also search for compositions not well-represented in the meteorite collection, such as evolved, K-rich lithologies [4], and outcrops of olivine from Vesta s mantle or igneous intrusions in major impact basins [5]. The search for a possible mesosiderite source region is described in [6]. GRaND will globally map the abundance of

  17. La grande vitesse est-elle toujours sur les rails ?

    OpenAIRE

    Libourel, Eloïse; Ruggeri, Charlotte; Schorung, Matthieu

    2016-01-01

    National audience; Depuis le milieu du XXème, la grande vitesse ferroviaire est considérée comme une innovation révolutionnaire pour le transport de voyageurs. Elle renvoie une image de modernité à tous les acteurs institutionnels et à la société civile, et elle permet également aux États d’afficher une image de puissance technique et industrielle. L’objectif est de capitaliser sur l’objet « grande vitesse » pour valoriser l’avancée technologique d’un pays, d’une région, et pour promouvoir de...

  18. Prigionieri dimenticati. Italiani nei lager della Grande guerra

    Directory of Open Access Journals (Sweden)

    Andrea Montanari

    2013-10-01

    Full Text Available L’articolo tratta del ciclo di eventi “Prigionieri dimenticati. Italiani nei lager della Grande guerra”, allestiti a Bibbiano (Reggio Emilia dal 15 al 29 settembre 2013. La prigionia nel campo di Celle (Germania è al centro di: una mostra, composta da pannelli esplicativi e oggetti provenienti dai lager e dalle trincee; uno spettacolo teatrale intitolato “Sandrone soldato”, scritto proprio a Celle; e un concerto di musiche originali composte e cantate nei lager e nelle trincee. La Grande guerra, dunque, raccontata in modo del tutto originale.

  19. Noncommutative geometry, Grand Symmetry and twisted spectral triple

    CERN Document Server

    Devastato, Agostino

    2015-01-01

    In the noncommutative geometry approach to the standard model we discuss the possibility to derive the extra scalar field sv- initially suggested by particle physicist to stabilize the electroweak vacuum - from a "grand algebra" that contains the usual standard model algebra. We introduce the Connes-Moscovici twisted spectral triples for the Grand Symmetry model, to cure a technical problem, that is the appearance, together with the field sv, of unbounded vectorial terms. The twist makes these terms bounded, and also permits to understand the breaking making the computation of the Higgs mass compatible with the 126 GeV experimental value.

  20. A World of Love 2008 Music Grand Ceremony

    Institute of Scientific and Technical Information of China (English)

    Bai; Yifeng

    2008-01-01

      Under the guidance of the theme slogan of the 29th Olympic Games - One World One Dream, with dream, love and dedication as its theme, the 2008 Music Grand Ceremony & 1000 People Charity Concert on 100 days countdown to Paralympic Games is officially launched on December 22, 2007. The 2008 Music Grand Ceremony is sponsored by China Disabled Persons' Federation, and co-hosted by National Paralympic Committee of China, China Huaxia Culture Group and Beijing Hanbohongyuan Culture Development Co., Ltd., aiming at supporting Chinese paralympic delegation in the 2008 Paralympic Games.……